LOCUS       DASRKK010000001         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_684163, whole genome shotgun sequence.
ACCESSION   DASRKK010000001 DASRKK010000000
VERSION     DASRKK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684163"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFC15_00005"
     CDS             complement(<1..337)
                     /locus_tag="VFC15_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZM08576.1"
                     /translation="MSSATLVSVQEYLATRYRPDRDFVDGELQERNLGELEHALLQSA
                     ILAWFWAKQREWNVLPIVEQRVQVAATRFRIPDISVLRADQPREPIVTTPPLILIEVL
                     SKDDTLRSMR"
     gene            complement(439..981)
                     /locus_tag="VFC15_00010"
     CDS             complement(439..981)
                     /locus_tag="VFC15_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZM08577.1"
                     /translation="MTYKLVIRTLAVATALTLCVASGFAQAKPVKKKTVELKDAKGND
                     VGTATIVSKGAGVEVKLALKDLPPGEHAVHFHQNAKCDPPDFKSAGPHFNPTGKQHGL
                     ENPNGHHAGDMPNFTVKENGTAKATVKDEDVVLGTGSESNSLFANGGTAMMVHAKADD
                     MKTDPAGNAGDRIACGAITP"
     gene            complement(1005..1232)
                     /locus_tag="VFC15_00015"
     CDS             complement(1005..1232)
                     /locus_tag="VFC15_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08578.1"
                     /translation="MLRTIQLRNVPDALHRTLKARAAAAGMLLSDYLLSEIREVAERP
                     TLTELQQRLQRRQPVDLPVPAAAAVRAGRDA"
     gene            1377..2315
                     /locus_tag="VFC15_00020"
     CDS             1377..2315
                     /locus_tag="VFC15_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZM08579.1"
                     /translation="MAVNPVMLVHGGAWAIPDDALEAHLQGVQAAAAQGWQVLQRGGS
                     CLDAVEAAVVAMEEDEAFDAGRGSFLNMDGRVQLDALMMDGATLRAGGVGCVEHIRNP
                     IRAAKKVLTESPHVYFVATGAERFAQEHGIELCSNDELVIEREVLRLREAKKQSYLEM
                     PDEFGPMRSSDTVGAVALDRDGNIAAATSTGGTLNKAPGRVGDSSLIGCGCYADNRSA
                     AVSTTGWGEPMMKLVLSKWAADRVEDGLVPQEAAQRALYYLKARLNGHGGIIIVDARG
                     RIGLAHNTPRMAWSLTTKDGVSSGIQVGTTSSGCTP"
     gene            complement(2464..>5133)
                     /locus_tag="VFC15_00025"
     CDS             complement(2464..>5133)
                     /locus_tag="VFC15_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM08580.1"
                     /translation="RTLMALASGTKLGPYEIVSPLGAGGMGEVYLARDTRLNRSVAVK
                     ILPSHLSKDPEAKQRFDREARTISSLNHPNICTLHDVGHQDGIDYLVMEHLEGQTLAE
                     RLSKGPLPLDQVLKYGAEICEGLERAHRSSVVHRDLKPANIMLTKTGAKLMDFGLAKQ
                     SVASATAASGLTATAKTPQGSQPLTAQGTVVGTFQYMSPEQIEGKEADARSDIFALGA
                     VLYEMVTGKRAFEGKTPASAMAAVLEREPTPISLVQPMTPPALERLVKICLSKDPDDR
                     WQTAHDVGLQLKQIAEGASQASAPGMPVPQHKRDNAWVWVLTGILGVVAGVALVLAYL
                     ASHRDVRVLRVELNPPDKMQFNLSGDHGGPAMISPDGRYIIFSAYGSGGAQLYLRSLD
                     STSPQALSGTEGAMFPFWSPDSRSVAFFTDDKLKRIEVSGGTPVTICGSTLGRGGSWN
                     QDGTIVAALSYNTGLSQVPASGGTPTPITMIDGVHYSSHRWPWFLPDGKHFLYIAVKH
                     NAPTSPETAVFLASLDGKENRLLFHTLSNPIYASGRLLFQRENSLVAQSFDPSNGKFS
                     GEPQTLSENVQFDPGLWRMNLSVSTDGMMLYASGTASGTEILTWYDRSGKRLGTVGEQ
                     GEFYDLDLSPDEKKVATTELNTAIATIWIRDLASNLKTRLTFSDGPHLTPLWSPDGKE
                     VAFTSNQQAAISVKTIGSSAPERTLLSSPNPIYQAISDWSHDGRYLMYEQGTGVNTDL
                     WVLPLSGDSKPFPYTSGSSRGAFSPDGHWVAYVGQEGGRPEVFVAPFPWTGAKWQVSN
                     GGGAGPRWRADGKELFYFDFNGVTAVEVDGARSAFQVGSSKLLFRLPLRGIMSREYAP
                     SHDGQRFLAVTPSEGSSQSLTLVQNWPAELKNK"
BASE COUNT          982 a         1531 c         1576 g         1044 t
ORIGIN      
        1 ccctcatgct acggagggtg tcgtccttcg aaagaacttc gatcaggatc agcggaggtg
       61 tagtgacgat cggctcgcgt ggttgatcgg ctcgcaggac gctgatatct ggtatgcgga
      121 agcgagtcgc ggcaacctgc acccgctgtt ccacgatcgg caacacgttc cactctcttt
      181 gtttggccca aaaccacgcg agaatggcac tctgcaaaag ggcgtgttcc agctctccca
      241 agtttctctc ctgcaactct ccgtcgacaa agtcccggtc cggccggtac ctggtcgcaa
      301 gatattcctg caccgagact agcgtcgcgc tcgacataga caatcgcttt ccccccagta
      361 ttatgctccc cttccaatgc gcggccaaac cttttcagag agcgtttggg aggctcctta
      421 aagagacacc gcccaatcct acggcgtgat cgccccgcac gcgatacggt cgccggcatt
      481 gcccgcggga tcggtcttca tgtcgtcggc tttagcgtgg accatcatcg ctgtccctcc
      541 gttagcgaac agagagttcg actcgctgcc tgtgccgaga acgacgtcct catctttcac
      601 agtcgccttc gccgtcccgt tttccttgac ggtgaagttc ggcatgtcac cggcatggtg
      661 gccgttcggg ttctccagac cgtgctgctt gcccgtggga ttgaagtgcg gcccagccga
      721 cttgaagtcg ggcggatcgc acttggcatt ttgatggaag tgcaccgcgt gctcgcccgg
      781 aggcaggtcc ttgagcgcca gcttcacttc cacgcccgct cccttggaga cgatggttgc
      841 ggtgccgaca tcgttaccct tcgcgtcctt cagttcgacg gtcttcttct tgaccggttt
      901 cgcttgagcg aatcccgagg caacgcagag agtgagtgct gtagcaacgg cgagagtgcg
      961 aatcaccagc ttgtatgtca taacgtcaga gttttaccac gaaactacgc gtcacgccca
     1021 gcgcgcacgg cggcggctgc cggtaccggt aaatcgacgg gctggcgccg ctgcaggcgc
     1081 tgctgcagct cggtcagggt tgggcgctct gcgacctcgc ggatttcgga gagcaggtag
     1141 tccgaaagca acatgcctgc tgcagccgcg cgggccttaa gggtgcgatg cagagcgtcg
     1201 ggaacattgc gcagctggat cgtgcgtaac atatgctcag catattacat gcgtgtcata
     1261 tattcacctt ggggcagacg tggtctcgcc ttccgtggat agcgccctga cgccattctg
     1321 gtatgctttt tggtttgccc tgcggcaaca atggcaccgc aacgaaaggt actgatttgg
     1381 cagtcaatcc tgtaatgctg gtccacggtg gggcttgggc aatccccgac gatgcgctcg
     1441 aggcgcactt gcaaggggtg caagccgcag ctgcccaagg ctggcaggtg ctgcaacgcg
     1501 gcggaagctg ccttgacgcg gttgaggcgg cggtggtcgc catggaagag gacgaggcct
     1561 tcgacgccgg tcgcggctct tttctcaaca tggatggccg ggtgcaactg gacgcgctga
     1621 tgatggacgg agcgacgctg cgcgccgggg gcgtgggttg cgtggagcac attcgcaacc
     1681 cgatccgtgc cgccaagaag gtgctgaccg agagcccgca cgtgtacttc gtcgcaaccg
     1741 gagcagaacg gtttgctcag gagcacggca tcgagttgtg ttccaacgac gagctggtca
     1801 tcgagcgtga ggtactgcgc ctgcgcgaag ccaagaaaca gagttacctc gagatgcccg
     1861 acgagttcgg ccccatgcgc tcgagcgaca cggttggggc ggtcgcgctc gatcgcgacg
     1921 gcaacattgc ggctgccact tcgaccggcg gaacgctgaa caaggcgccg ggcagagtgg
     1981 gcgattcgtc gctgatcggc tgtggctgtt atgccgacaa ccgcagtgcc gcggtttcca
     2041 cgacaggatg gggcgaaccc atgatgaagc tagttctcag caagtgggcc gctgaccgcg
     2101 tggaggatgg gcttgttcca caagaggccg cgcaacgcgc gctctactac ctaaaggccc
     2161 gactgaacgg ccatggagga atcatcatcg tggatgccag aggaagaatc ggcctggccc
     2221 acaatacgcc tcgtatggcg tggtctttga ccacgaaaga cggcgtttcc tccggtatcc
     2281 aagtcgggac tacgagttct gggtgtacgc cgtaaccgtg gaagcgcccg gtttcgttta
     2341 gcgattggag cggatggacc catcgcctcc tcgcagggcc acttcgacag ctccgaatac
     2401 caacgtcaac tagagcacag tgtccaggac ccgcgataga cgctccacgc catatagcca
     2461 gcactacttg ttcttcaact ctgccggcca gttctggact agagtcaacg actgagagct
     2521 gccttcactg ggcgtgaccg cgaggaaccg ctgaccatcg tgcgagggag catactcacg
     2581 agacatgatg cctctcagag gaaggcggaa cagcaactta ctgctgccga cctggaatgc
     2641 ggacctcgcg ccgtccacct caaccgccgt aacgccgttg aaatcgaagt agaagagttc
     2701 cttgccgtca gctcgccaac gcggtccggc gccgcctccg ttcgaaactt gccacttcgc
     2761 accggtccaa ggaaatgggg ccacaaaaac ctcgggacgg cccccttctt ggcccacata
     2821 tgccacccaa tggccatccg gcgaaaacgc tcctcttgac gacccgctcg tatagggaaa
     2881 cggcttgctg tcgccggata acggaagcac ccaaaggtcc gtattcacgc cggtaccctg
     2941 ttcatacatc aggtagcggc cgtcgtgcga ccagtccgaa atggcttgat agataggatt
     3001 cggcgacgaa agaagcgtcc gctccggggc gctgctgccg atcgtcttaa ctgagatcgc
     3061 ggcctgctgg ttcgaggtga aggctacttc ttttccgtcc ggagaccaaa gcggagtcag
     3121 gtgtggaccg tcgctgaagg tgagccgagt cttcagattg cttgccaggt cgcgaatcca
     3181 gattgtcgcg atggctgtgt tcagctccgt ggtcgcgact ttcttctcgt cgggggagag
     3241 atccagatcg tagaattccc cctgctcgcc taccgtgccc agccgctttc cgctgcggtc
     3301 gtaccaggtt aggatctcgg tcccggaagc cgtgccggat gcgtacaaca tcatgccgtc
     3361 ggtggagacg ctgaggttca tccgccacag cccggggtca aattgaacgt tttcacttag
     3421 ggtctgcggc tcgccggaga atttgccgtt ggatgggtcg aagctctgtg ccaccaggga
     3481 attttcgcgc tgaaagagta agcgtcccga cgcgtagatg gggttcgata aggtgtgaaa
     3541 cagaaggcgg ttctctttgc cgtcgagcga tgcaaggaac accgcggttt ccgggctggt
     3601 gggcgcatta tgtttaacgg cgatgtagag gaagtgcttg ccgtcaggca agaaccacgg
     3661 ccagcggtga gaggaatagt ggacgccatc gatcatggtg ataggagtag gcgtgcctcc
     3721 gctggctggg acctgcgata gaccggtatt gtaggagagg gcggccacaa tggttccgtc
     3781 ctggttccaa ctgccgccac gtcccagcgt cgaaccgcag atcgtgaccg gcgtgccacc
     3841 gctcacttcg attcgtttca gcttgtcgtc ggtgaagaag gccacggagc gactgtcggg
     3901 cgaccagaaa gggaacattg caccctccgt gcctgaaagg gcctgcggag aagttgaatc
     3961 cagcgaccgc aaatagagct gggcgccccc ggagccgtag gccgaaaaga tgatgtaacg
     4021 gccgtcagga gaaatcatcg ccgggccgcc gtgatcgcct gacaggttga actgcatctt
     4081 gtcgggcggg ttgagttcga cccgcaatac ccgcacgtct ctatggctcg ctagataggc
     4141 cagcaccagc gccacaccgg caactacccc tagaatccca gtcaggaccc agacccacgc
     4201 attatcccgc ttatgctgcg gtaccggcat cccgggcgcg ctggcctgcg atgcaccctc
     4261 ggcgatctgc ttgagttgca gcccaacgtc gtgcgccgtt tgccaacggt cgtcgggatc
     4321 cttgctcagg catatcttga ccagacgttc tagcgcgggc ggggtcatcg gttgcaccaa
     4381 agaaatcgga gtcggctcgc gctccagcac cgccgccatc gcgctggccg gagtcttgcc
     4441 ctcgaaggcc cgctttcccg ttaccatctc atagagcacc gcgcccaggg cgaagatgtc
     4501 cgagcgtgca tcggcctctt tcccctcgat ctgctcgggc gacatgtact ggaacgttcc
     4561 cactacggtg ccctgcgcgg tgagaggttg acttccctgg ggtgtcttcg cggttgcggt
     4621 gaggccggat gccgcggtcg cgcttgcaac cgactgcttg gccagaccga agtccatcag
     4681 cttggccccg gtcttggtca gcatgatgtt ggcgggcttc aagtcacggt gcaccacact
     4741 gctgcgatgt gccctctcca ggccttcgca gatttcggcc ccgtacttca agacctggtc
     4801 gaggggcagc ggtcccttgc ttaaacgctc tgccagcgtc tggccttcca ggtgttccat
     4861 caccaggtaa tcgatcccat cctggtgtcc aacgtcgtgg agcgtacaga tattgggatg
     4921 attcagggaa gagatcgtcc gggcttcccg gtcgaagcgt tgtttggcct ctgggtcttt
     4981 agaaagatgt gagggaagaa ttttgaccgc gacgctacgg ttgaggcgtg tatcgcgcgc
     5041 aaggtacacc tctcccatgc cgccagcgcc cagcggcgag acgatttcat aaggaccaag
     5101 tttggtgccg gaggcgaggg ccatcagtgt gcg
//
LOCUS       DASRKK010000002         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_684213, whole genome shotgun sequence.
ACCESSION   DASRKK010000002 DASRKK010000000
VERSION     DASRKK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684213"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFC15_00030"
     CDS             <1..1147
                     /locus_tag="VFC15_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZM08581.1"
                     /translation="TLERIAENPDDFYHGAMARELVAAIEKGGGLITAEDLAQYEVKE
                     RQPIRGTYRGYEVISAPPPSSGGVTLVESLNILEGYDLGKQGEGSAQNIHLTAEAYER
                     AFFDRAEFLGDPDFSKIPVAQLLDKRYANAWRETIPLRRATPGNELRRPSVFSQLDSY
                     ASSHPQPKLLLEPVHTTHYSVVDPEGNAVSVTTTLNDDFGSAITAEGLGFLLNNEMDD
                     FTTKPGAANLYGLIQGPANAIGPGKRPLSTMAPTIVLKGGKLFLVLGAEGGPTIVTTV
                     ANVLMGVVDYGLNIQQAVNAPRFHDQWLPDEIKLEKVGFSPDTVRILEHMGHKVSADA
                     NLSSPPAHQLRSKVEYWGDAQCIEVDMDTGERLGASDGRKNGKAVGF"
     gene            1159..1974
                     /locus_tag="VFC15_00035"
     CDS             1159..1974
                     /locus_tag="VFC15_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZM08582.1"
                     /translation="MSTLIYARRERLHPGLLDQMVAGGAEAIEIFAARGHFNYTDRQH
                     IRQIANWFRSTGVTFHSMHSPMHPDEDWGRGGATHINIASVEKLQRVAATDEIKRAIE
                     VAELAPFQFLVQHVGIGHEEADEHKLDAAMSSLEHLHAFAKPLGVQLLVENITNELST
                     PETLLDLLHAGRFDDIGVCFDVGHAHIMSSVPQAFEVLKDRIRSTHVHDNNQERDSHL
                     WPGDGTIDWTETLSLLRSAPHVPPVLLEIEGVEGENISHRMATAFAKLELRPE"
     gene            1994..>2274
                     /locus_tag="VFC15_00040"
     CDS             1994..>2274
                     /locus_tag="VFC15_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08583.1"
                     /translation="MLVPLLFLLFSMKPQAILGPMDVPKNTQQIVLVETDSWTSSTGE
                     LQMLSRSPHGWIAVGAKIPVSVGQKGLAWGRGLQQPITEGPQKVEGDGKA"
BASE COUNT          498 a          672 c          666 g          438 t
ORIGIN      
        1 gactctggag cgcatcgccg aaaatcctga cgacttctac catggcgcga tggcgcgtga
       61 gctggttgcg gccatcgaga agggcggcgg cctgatcacc gccgaggacc ttgcccagta
      121 cgaggtgaag gagcggcagc caattcgtgg cacctatcgc gggtacgagg tcatcagcgc
      181 tccacctccg tcttctggag gggtcaccct ggtcgagagc ctcaacatcc ttgaaggata
      241 cgacctgggt aagcagggtg aaggttcggc gcagaacatt catctcactg ccgaagctta
      301 cgagcgtgca tttttcgacc gcgccgagtt tctcggagac cccgatttct ccaagatccc
      361 ggttgcgcaa ctgctggaca agcgatacgc caatgcctgg cgcgagacca tcccgctgcg
      421 ccgtgccact cccggcaacg aactgcggcg tccttctgtc ttctcgcaac ttgacagcta
      481 tgccagttcg catccgcagc caaaattgtt gctcgagcca gtccatacta cccactattc
      541 ggtagtcgat cccgagggca acgcggtgtc cgtcacaact acgctgaacg acgatttcgg
      601 ctcggccatc acagccgaag gactgggatt cttgctcaat aacgagatgg atgacttcac
      661 cactaagccg ggggctgcaa acctttacgg attgatccag ggccccgcca acgcaatcgg
      721 cccaggcaag cgacccctct ccaccatggc gccgaccatt gttcttaagg gcggcaagct
      781 ctttctggtc ctcggcgctg agggcggtcc caccatagtc accacagtcg ccaatgtcct
      841 aatgggcgtc gtggattacg gactgaacat tcagcaagca gtcaatgcgc cgcgctttca
      901 tgaccagtgg ctgcccgatg agatcaagct ggagaaggtc ggattctcac ccgacacggt
      961 ccgcatcctc gagcacatgg gacacaaagt atccgccgac gcaaacctca gctcaccgcc
     1021 ggcccaccag ttgcgttcga aagtggaata ttggggcgac gcgcagtgca tcgaggttga
     1081 catggacaca ggtgaacgct tgggcgccag cgatggccga aagaacggaa aggcggttgg
     1141 attctgatgc gcaaggcgat gtccactctg atctatgcga ggcgggagcg gctgcatcct
     1201 ggcctactcg accagatggt cgcaggcggg gcggaagcca ttgaaatctt cgccgcgcgc
     1261 gggcacttca actacactga ccggcagcac atccggcaga tcgccaactg gtttcgcagc
     1321 accggagtaa cgtttcactc gatgcactcg cccatgcatc ccgacgaaga ttggggacgc
     1381 ggcggcgcga cgcacatcaa cattgcgtcg gttgagaaac ttcagcgtgt agcggcaacg
     1441 gatgaaatca agcgcgccat cgaggtagcc gagctggcgc catttcagtt tctggtgcaa
     1501 catgtcggca tcggccacga agaggcggac gagcacaaac tggatgcggc aatgagctcc
     1561 ctcgaacacc tgcacgcttt cgccaaacct ctgggcgtgc aactgctggt ggagaacatt
     1621 accaatgagc tttcgacgcc ggagacgttg ctggatctgc tgcatgccgg gcgcttcgac
     1681 gatatcggcg tctgttttga cgtcggccac gcgcacatca tgtcgagcgt gccgcaagcg
     1741 ttcgaggtgc tcaaagaccg catccgctcg acgcacgtgc acgacaacaa tcaggagcgc
     1801 gattcgcacc tatggcccgg cgacggcacc attgattgga ccgagaccct gtcgctcttg
     1861 agaagtgcac cacatgtgcc gccggttttg ctggagattg agggcgtgga gggcgagaac
     1921 atatcccata gaatggccac agccttcgcc aaattagaac tgcgtcctga gtagtttggt
     1981 ggcaagaacg cttatgctag ttcccttgct tttcctattg ttcagtatga aaccacaagc
     2041 cattctcggg cccatggatg tgcccaagaa cacgcagcag attgtactgg tcgagacgga
     2101 ttcgtggacc agcagcactg gtgaactgca gatgctgtcc cgatctcctc atggctggat
     2161 cgccgtgggc gccaagattc cggtttccgt tggtcagaaa gggctggcgt ggggaagagg
     2221 gctccagcaa cccattactg agggcccaca aaaagtggaa ggggatggca aagc
//
LOCUS       DASRKK010000003         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_977547, whole genome shotgun sequence.
ACCESSION   DASRKK010000003 DASRKK010000000
VERSION     DASRKK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977547"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VFC15_00045"
     CDS             complement(<1..1501)
                     /locus_tag="VFC15_00045"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZM08584.1"
                     /translation="MATTTTPAREGTRISATINNAQIPATFQNEPSVDFSNPENARRM
                     KAAIEKVRNELGREYDLVIGGRHLKTAEKTQSLNPAKPSEIVGIHQAAGASEVEPAMQ
                     AALKAFETWKFSSIEERTNLLFRVSALLRERKFELMAWMIFEVGKNWAEADGDIAELI
                     DFCDFYALEALRLSKAEAPVQLPGERDYLRYIPLGVGVVIPPWNFPGAIMGGMTLASI
                     VSGNTVILKPSSDSPTIAAKFFELLEEAGLPDGVVNFCPGHGNSFGNTIVEHPKTRYI
                     AFTGSKEVGLHINQRAAVQQPGQIWIKRTILEMGGKDSIVVDTDADLDAAVEGVASAA
                     FGFSGQKCSACSRAIVDERVYDKFLQRLKERVEKITVGDPTQNPGMGPVVNEKSMQSI
                     LGYIEKGIAEGGRLITGGARATEAGDGFFIQPTVIADVEPMATISQQEIFGPVLAVIR
                     SRGFEHGLEIANNTEFGLTGAVYTTSEDKIEAAKEQFHVGNLYINRKCTG"
     gene            1708..2100
                     /gene="tcmP"
                     /locus_tag="VFC15_00050"
     CDS             1708..2100
                     /gene="tcmP"
                     /locus_tag="VFC15_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HZM08585.1"
                     /translation="MDSTAGTDALPHIKRVSRIKHAILEKYLPPWAIILGSANRRLNY
                     FDCFAGPGRYEFDGEAVDGSPVIAVRASKAFLGARPSHSLNVILTEKDKHQAQQLERQ
                     LGLLTPYPKNLRVEVLQEDSSTFIPDLS"
     gene            2105..2815
                     /gene="tcmP"
                     /locus_tag="VFC15_00055"
     CDS             2105..2815
                     /gene="tcmP"
                     /locus_tag="VFC15_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HZM08586.1"
                     /translation="MQSLAPSFFLIDPYGHPLSLPVINEILSRSHTEVFINLMWYRIN
                     MDLSNPAVQGHVDRLFGNQQWRQMPFMEQTGTAREEGFLEYFVSQLRAKYVLRFRIGF
                     DPEDKRMKGERTKYYLLHASNHSRAALLMKEVMWPLGDEDGTFDFSGESQGVLISRTP
                     KVDELREILPRVFAGREIQFDNIREETWNLPFIEKHYREALQKLRSEGIVTVTPITSK
                     KSGLRQRDMVRFPNAAKS"
     gene            2846..>3325
                     /locus_tag="VFC15_00060"
     CDS             2846..>3325
                     /locus_tag="VFC15_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HZM08587.1"
                     /translation="MSLQSKIEWTDATWNPVRGCTKISPGCAHCYAAVFAERFRGVKG
                     HPYEQGFDIRLVPEKLNEPLKWRSPRRVFVNSMSDLFHEAVPDDYIASVVDVMVQADW
                     HTFQVLTKRSRRMRDLLKTRLRFAAAKPHIWWGVSVENKKHGLPRIEHLHSAPAAVKF
                     "
BASE COUNT          698 a          925 c          959 g          743 t
ORIGIN      
        1 ctcccgtgca tttgcgattg atgtacaggt tgccgacatg gaactgctcc ttggccgctt
       61 cgatcttgtc ttccgacgtg gtatagaccg cgccggtcag gccgaactcg gtgttgttgg
      121 cgatttcgag gccatgttcg aagccgcgcg atctgatcac cgcgagcacc ggaccgaaga
      181 tttcctgctg cgaaatcgtc gccatgggct cgacatcggc aatcacggtg ggctggatga
      241 agaaaccatc gcccgcttcg gtcgcgcgcg cgccgccggt aatcaggcgc ccaccttcgg
      301 cgatgccctt ctcgatatag cccagaatcg actgcatgga tttctcgttg accaccgggc
      361 ccattccagg gttctgcgtg ggatcgccaa ccgtgatttt ctcgacgcgc tccttcaggc
      421 gttgcaggaa tttgtcgtac acgcgctcat cgacaatggc gcgcgagcac gccgagcatt
      481 tctgcccgct gaatccaaac gccgccgagg ccacgccctc gaccgcggca tcgagatcgg
      541 cgtcggtgtc caccacgatg gagtccttgc cgcccatctc aaggatggtg cgcttgatcc
      601 aaatctggcc gggttgctgc accgcggcgc gctggttgat gtgcaggccc acttccttcg
      661 atccggtgaa cgcgatatag cgcgttttgg gatgctcgac gatggtattg ccgaagctgt
      721 tgccatggcc ggggcagaag ttgaccacgc cgtcggggag gccggcttct tccaggagct
      781 cgaaaaattt cgcggcgatg gtgggggagt cgctcgacgg ctttaggatg accgtgttgc
      841 cactcacgat ggaggccagc gtcatgccgc ccatgatggc gccgggaaag ttccacggcg
      901 gaatcaccac gcccacaccc agcgggatgt agcgcaggta gtcgcgctcg cccggcaact
      961 gtaccggtgc ctcggccttg gagaggcgaa gcgcttccaa ggcgtaaaag tcgcagaagt
     1021 caatcagctc ggcgatgtcg ccgtcggcct cggcccagtt cttgccgacc tcaaagatca
     1081 tccacgccat cagctcaaac ttacgctcgc gcagcagcgc gctcacccgg aacagcaggt
     1141 tggtgcgctc ctcgatcgag gaaaatttcc aggtctcgaa cgccttcaac gcggcctgca
     1201 tcgcgggctc aacttcgctt gcgcccgccg cctgatgaat gccgacaatc tccgacggct
     1261 tggccggatt gagcgattgc gtcttctcgg ccgtcttcag atggcggccg ccgatgacca
     1321 ggtcgtactc gcggcccagt tcgttgcgga ctttctcgat ggccgccttc atgcggcgcg
     1381 cgttctccgg attgctgaaa tcaaccgagg gttcgttctg gaaggtggcg ggaatctggg
     1441 cgttattgat ggtggcggaa atgcgagttc cttcccgggc gggagtcgtt gtcgtagcca
     1501 taagtgcgtc atctcaggtg tttatcagtg cagttttaat agatgccgtg cgcttcggcg
     1561 ggatgcggcg cgagtgcatg ccaaccctaa tgataagcgc ggccgggggg tttgtcttgc
     1621 gaagccctgc gagatccgcg gttctgtttc ggagttgaca agcattgcat cgtccgctat
     1681 tcttggcgaa gaaaaggcga acctacgatg gactcgacgg ctggaactga tgctcttcct
     1741 cacatcaaac gcgtttcgcg gatcaaacac gccatcctcg agaagtattt accgccatgg
     1801 gcgatcatcc tcggttcagc gaaccgccgc ctgaactact ttgattgctt cgccggacct
     1861 ggacgctacg agtttgacgg cgaagcggtc gacggctcac ccgtaattgc cgtaagagcg
     1921 tccaaagcat ttttgggggc acggccaagc cactccttga acgtaattct gaccgaaaaa
     1981 gacaagcacc aagcacagca gcttgagcga caattgggcc tccttacgcc gtatccaaag
     2041 aatctgcgcg tagaggtact tcaggaagac agcagcactt ttatccccga cctttcttag
     2101 tagggtgcag tctctcgctc catctttctt cctcatcgat ccgtacggtc accctctgtc
     2161 gttgccggtc atcaacgaga tactttctcg ctcgcatacg gaggttttca tcaatctgat
     2221 gtggtatcgc atcaacatgg acctaagtaa ccctgctgtt cagggacacg tggatcgact
     2281 attcggcaac caacagtggc gtcagatgcc tttcatggag cagactggca cagctcgcga
     2341 ggagggtttc ttggagtatt tcgtttcaca actaagggca aagtatgtcc tgcgatttcg
     2401 gataggcttc gatcccgaag acaagagaat gaaaggggaa cggacgaaat actacttatt
     2461 gcatgccagc aatcactcgc gagccgccct gttgatgaaa gaggtgatgt ggccgcttgg
     2521 cgatgaagat gggacgttcg atttttcggg ggagtcccag ggtgtcctaa tctcccggac
     2581 cccaaaagtt gatgaacttc gtgaaatatt gcctcgagtc tttgcgggcc gcgaaattca
     2641 gttcgacaat attcgtgagg agacttggaa tctgcctttc atcgagaaac attatcgcga
     2701 ggccctgcag aagctacgat ccgaagggat cgtgaccgtt acccccatta cttcaaaaaa
     2761 gagtgggtta cgccaaagag acatggtacg ttttccaaac gcagctaaat cctaggggaa
     2821 ataagtagag tttggtcgga atcagatgag ccttcagtca aagatcgaat ggacggacgc
     2881 gacgtggaac ccggttcgtg gctgcactaa gatcagtccg ggctgcgccc attgctacgc
     2941 cgctgtgttt gctgaacgat tccggggcgt caaaggccat ccctacgagc aagggtttga
     3001 tattaggctc gtgccggaga aactgaatga gcctttgaaa tggcgctcac ccagacgcgt
     3061 attcgtcaat tccatgagcg acttgtttca tgaggctgtt ccagatgact acattgcttc
     3121 tgttgtggat gtgatggtcc aggcagattg gcatacgttc caggtgctga cgaagcggtc
     3181 ccggcgtatg cgtgacctgc taaaaacacg tctgaggttt gcagcagcga agccgcacat
     3241 ctggtgggga gtcagtgtcg aaaataaaaa acatgggctg cctaggatcg agcacctgca
     3301 ctctgcacca gcagcggtaa aattc
//
LOCUS       DASRKK010000004         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_283, whole genome shotgun sequence.
ACCESSION   DASRKK010000004 DASRKK010000000
VERSION     DASRKK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFC15_00065"
     CDS             <1..471
                     /locus_tag="VFC15_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08588.1"
                     /translation="LSAGHSMNDSPQEQSSGRIVYAQLVAFRHSTQKNVYVLASHSHF
                     VMNDIYATACHASGDVLPGWIVGSAGAVRYRLPQDHAAASVAMTNVYGYVLATVAPDG
                     SIAFEFKEVKEADVPASAVNEFSREQVNWCFTQNKASYTPAGAVCPAGPTPTGH"
     gene            complement(482..1597)
                     /locus_tag="VFC15_00070"
     CDS             complement(482..1597)
                     /locus_tag="VFC15_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZM08589.1"
                     /translation="MTGEVTRTQIAPPGPGATLLYDDWYPALRSSKIAGKRMATAMLL
                     GIPLVLGRTAAGRYFAMRDTCPHRGIPLSYGWFDGRQVTCKYHGWSFEAVSGECREIP
                     SLTNHDSLDPSRIYAAAFPCEEHDGHVWVYVPQPGRGKLRMDDAAKLPPVPEVPKFSQ
                     RYRSAHLSADLPCNVDHGIIGLMDPAHGPFVHQAWWWRSKQSIHEKQKQFEPIDSGFR
                     MTAHQPSANSAPYKLLGVYGQPITTTIDFVLPNRRYEIIRCGEKWFASLTTVTPTTED
                     HCRIDVCAAWNVFLRVPFLTAIAKFFGRRFVRQDQLTMIQQAEGLKHNPSLMLIDDAD
                     RPAKWYFELKQARLESQATGAPMRHPMSGPVTLRWRS"
     gene            1862..>2427
                     /locus_tag="VFC15_00075"
     CDS             1862..>2427
                     /locus_tag="VFC15_00075"
                     /inference="COORDINATES: protein motif:HMM:NF021285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZM08590.1"
                     /translation="MHKNITNRFLRGFVGNSSVTGAHPLDLLIAGMSRRSKMFSRGWG
                     EESLLTELSTRVSYSDPPPPISLEWHSARKRDRIVRRDGTFASPLELLPKETRTVHVR
                     AWLREGNRAACVILAGSRDEGYRPREHVFGPLIERGLDLYFLVSPFYGRRRLAGGPSL
                     ITVSDHGLMALGMVLEARALLENLSERYP"
BASE COUNT          464 a          707 c          752 g          504 t
ORIGIN      
        1 ttatccgccg ggcacagcat gaacgactcc ccgcaagagc aaagcagcgg gcggattgtc
       61 tatgctcaac tggtggcctt tcggcacagc acccagaaga acgtgtacgt gctggcgagc
      121 cattcgcact tcgtgatgaa cgacatctac gccaccgcct gccatgccag cggcgatgtg
      181 ttgcccggat ggattgttgg ctctgccggc gccgtgcgct atcgcctgcc gcaggaccac
      241 gctgcggcca gcgttgccat gaccaatgtt tatgggtatg tgctggctac ggttgcgccg
      301 gacggaagca tcgcgttcga gttcaaggaa gtcaaagaag cggatgtgcc ggcaagcgcg
      361 gtcaatgagt tctcgcgtga gcaggtgaat tggtgcttta cgcaaaacaa agcgtcctac
      421 actccggctg gcgccgtgtg ccctgcgggt cccacgccga caggccattg aacttcaccg
      481 ctcagcttct ccatcgcagc gtcaccggcc cgctcatggg atgtcgcatg ggagcgcccg
      541 tggcttgcga ttccagccgc gcctgtttca actcgaaata ccatttagct ggccggtccg
      601 catcgtcaat cagcatgagg ctgggattat gtttcagtcc ttccgcctgc tggatcatgg
      661 ttagttggtc ctggcgaacg aaccgtctgc cgaagaactt ggcgatcgcg gtaagaaacg
      721 gcacgcggag aaagacgttc cacgccgcgc agacatcgat acggcaatgg tcctcggttg
      781 tcggcgtgac cgtggtgagg ctggcgaacc atttctcgcc gcagcggatg atttcgtaac
      841 gccgattggg caagacgaag tcgatggtcg tggtgatggg ctgaccgtaa actcccagca
      901 gcttgtaggg cgcactgttg gccgacggct gatgcgcggt catgcggaag ccgctgtcga
      961 tgggctcgaa ctgtttttgc ttctcgtgga tactctgctt cgaacgccac caccaggcct
     1021 gatgaacgaa aggaccgtgc gcgggatcca tgagcccgat gatgccgtgg tccacgttac
     1081 agggcaagtc agccgacagg tgcgcgctgc gataccgctg gctgaacttg ggtacctctg
     1141 gaaccggcgg caacttcgca gcatcatcca tccgcagctt gcctctgccc ggttggggaa
     1201 cgtacaccca cacatggccg tcatgctcct cgcaaggaaa cgcagcagcg taaatacgtg
     1261 aggggtcgag ggagtcgtgg ttcgtgagcg aaggaatctc gcggcactcc ccagatacgg
     1321 cctcgaagga ccagccgtga tacttacagg taacctgccg tccatcgaac catccgtagg
     1381 aaagaggaat gccgcggtgg ggacaggtat cgcgcatagc gaagtaacgg cccgcggcgg
     1441 ttcgtcccaa aaccaatgga atgcccagca gcatggccgt tgccatgcgc ttgccggcta
     1501 tcttcgagga tcgcagcgcg ggataccagt cgtcgtatag cagcgtggcg cccggcccgg
     1561 gaggtgcgat ttgagtgcga gtgacttcgc cagtcatggt cctgagtccc catactatag
     1621 cggaaggcca gagttgtccg aggtcggagg agtcggcggc gaaaagatgg ctcgctcttc
     1681 cgttcgggac gccggtctgc ttttcgaggg tcaatgcccg gtgaataaca gactgttgat
     1741 tgctccgaca gggctgacgt tgtttgcgcc gggttcacaa agttctacct tccgtgtcag
     1801 gtttcgatta atatctgttg ccaacgttgt tgaaataacg cccatcgatt tcattactca
     1861 agtgcacaag aacataacca atcgcttcct tcgcgggttt gtcggcaata gttccgtcac
     1921 gggagcgcac ccactcgact tgctcatagc cgggatgtca cgccggtcga aaatgttttc
     1981 gcggggatgg ggagaggagt cgctcctgac ggagctttcg acgcgcgttt cctacagcga
     2041 tccgcctcct ccgatttccc tcgagtggca ctcggcgcgt aagcgcgacc gcatcgtgcg
     2101 gcgcgacggc acatttgcct cgccgctgga gttgctcccc aaagaaacgc gcaccgtaca
     2161 tgtgcgcgcc tggttgcgcg aaggtaatcg tgccgcctgt gtgatcctgg cgggttcgcg
     2221 tgacgagggt tatcggccac gcgagcacgt cttcggccca ctcatcgagc gagggctcga
     2281 cctctacttt ctcgtgagcc ctttctatgg ccgccggcgg cttgcgggcg gaccatctct
     2341 catcaccgtc agcgaccacg gcttgatggc gctggggatg gtgctggagg cgcgcgcgct
     2401 gctggaaaat ctgagcgagc gttatcc
//
LOCUS       DASRKK010000005         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1270791, whole genome shotgun sequence.
ACCESSION   DASRKK010000005 DASRKK010000000
VERSION     DASRKK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270791"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1539
                     /locus_tag="VFC15_00080"
     CDS             346..1539
                     /locus_tag="VFC15_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM08591.1"
                     /translation="MEKRLSGWKRELLRAHDLIAGLFARSETRARSLAYLQGLLSNCE
                     RKNGWQLAEWMGEASPYAVQHLLDRARWDADQVRDRIRSYALEELSSADAVLIVDETG
                     FLKKGRHSTGVKRQYSGTAGRIENSQVGVFLCYGCDKGAALVDRELYIPQEWAEDRER
                     RSEAGIPESLQFATKPELARRMIGRALDSGATAAWVAADEVYGHDSKLRRFLEGRQMA
                     YVLAVASDQRLWQADFMQHRVDEIARNLPASRWKRLSAGFGSKGERLYDWALMQLSKQ
                     DGWARALLVRRSIEEKPECAYYLCYAPSGKDTIETLVRVAGQRWKIEQAFETAKGECG
                     LDHYEVRHWQGWYRHITLSMLAHAVLSVLRARGEKNSRRASAAQRTGTAPPAHRNAVA
                     RMARH"
BASE COUNT          425 a          517 c          682 g          428 t
ORIGIN      
        1 tgctttacct gctcgaaact cgtggacaac gatgagtccc aattcgatgc ggggcttctg
       61 aagggatgga agtatatccg cgaacacgat gcacgaatgt ccattgggcg aactgcttcg
      121 ccggttcagg atacggactc atagcggaag tgccggaaaa ttcttgaatg ggctggcaag
      181 cgtgattaca acgcgccgac tgcgtattcc ggtgaagtgg gcacctgatt ccggtgatgc
      241 gggcaggcgt cggagcgaag cgacgctggt tgagtcttag tactacagtt gtagataaat
      301 gataagctag ttttgctctg gttttgtgat tctccggcct atgccatgga gaagcgactg
      361 tcaggatgga agcgggagtt gttgcgtgcg catgatctga tcgcgggttt gtttgcgcgt
      421 tcggagactc gtgcgcggag tcttgcgtat ttgcaggggt tgctgagcaa ctgtgaacgc
      481 aagaacggct ggcagttggc cgagtggatg ggcgaagcat ctccttatgc ggtccagcac
      541 ctgttggacc gggcgcggtg ggatgccgat caggtgcggg accggatacg cagttatgcg
      601 ctggaggagt tgtccagtgc cgatgcggtt ctgatcgtcg atgagacggg ttttctgaag
      661 aagggtcgcc actcgacggg cgtgaagcgt caatacagcg gcacggctgg acgcatcgag
      721 aacagccagg tgggcgtgtt tctctgctac ggctgtgaca agggcgcggc gctggtggac
      781 cgcgaactct acattccgca ggagtgggct gaggatcgag agcgtcgtag tgaggccggg
      841 attcccgagt cgctccagtt tgccaccaag ccggagttag cacggcgcat gatcggtcgc
      901 gcactggatt ccggcgccac ggcggcctgg gttgcggccg acgaggtgta cggccatgac
      961 agcaagttgc gtcggtttct ggaagggcga cagatggcct atgtgctggc cgtggcctcg
     1021 gaccagcgct tgtggcaagc ggattttatg cagcatcggg tagacgagat tgcgcggaat
     1081 ctgcccgcat ctcgatggaa gcgtctctct gccggcttcg gcagcaaggg cgaacggctc
     1141 tacgactggg cattgatgca attgtcgaaa caggatggtt gggcgcgggc gcttctggtg
     1201 cggcgcagca tcgaagagaa gccggagtgt gcttactatt tgtgttacgc ccccagcggt
     1261 aaagacacga ttgaaaccct ggtccgagtg gccggccaac gctggaagat cgagcaagcc
     1321 ttcgaaaccg cgaagggtga atgcggactg gatcactacg aggtgcgcca ctggcagggc
     1381 tggtatcggc atatcacgct gtccatgctg gcccatgcag tgttatctgt gttgcgagcg
     1441 cgcggagaaa aaaactcccg acgcgcaagt gcggctcagc gtaccggaac tgcgccacct
     1501 gctcacagga atgctgtggc gcggatggca cggcattgaa cacctgctgc attggtccca
     1561 gtggcgaaga cgacatcaat tccgggccat gtgctgccac tatcgcaagc gcggatcaac
     1621 cctgcctgca ttctatctac aactgtagta ctagaagctg cgctaccggc atttgccgga
     1681 gtagacttgc gagggcgaag gctggccaaa gaagcagaat cgggccatgg cgtgcacgcg
     1741 tcgtaccccg ttgcgcagtc ttgaatttca ttgaaagtat cggttggagc cggccttcgg
     1801 ctgcgttgtt acagattctc tcacggcgaa acatccggca gatcggttcg ggaaagaaaa
     1861 gataagtaga tcctctcaac agcgacagga gcagaaggat gactttgaca cgacgtgatt
     1921 ttcttctcag cacaacgtac gtgggcgcgg gcctcgccgt gactggcaca attgagcccc
     1981 tggtgacaca ggccgcggcg gagccgattg agttagcggg atatgtgacc ggaatccgca
     2041 tggccgcaac tc
//
LOCUS       DASRKK010000006         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_332, whole genome shotgun sequence.
ACCESSION   DASRKK010000006 DASRKK010000000
VERSION     DASRKK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..1940
                     /locus_tag="VFC15_00085"
     CDS             join(520..856,856..1940)
                     /locus_tag="VFC15_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM08592.1"
                     /translation="MARPDPEVVAKAKRRRFTGDYKRRILMEADAAKATGDLGSLLRR
                     EHLYSSLLVTWRRERDAGIVKGLTPSKRGPKSKQNPLDAEVSHLRRQNARLTEDLRKA
                     AIVIDVQKKLGNAAGEPSQSRNGREARMNAALHLASTVGVQSACEALGLVRASFYRQR
                     PLLGPTEQANPLRSDAIEAIEPTASPRALSSSERETVLACLHSERFQDRSPAAVYATL
                     LDEGVYHCSLRTMYRLLEEEGESGERRDQLTHPAYQKPELLATEPNQLWSWDITKLMG
                     AAKWTYYYLYVIMDVYSRYIVGWMVAPRETAELAKRLIEDTCHKQNIGRDQLTIHADR
                     GSSMTSKPVAFLLADLGVTKTHSRPHVSDDNPYSESQFRTMKYRPEFPDRFGSIQDSR
                     AFCQTFFAWYNDEHRHSGLGLLTPAMVHHGQAPLILAQRQNVLNAAFLAHPERFVRFA
                     PKPSPLPKEVWINKPPASVNNTQ"
BASE COUNT          458 a          680 c          569 g          414 t
ORIGIN      
        1 tccagtctgg ctctcagtcg gcccatggca acgtgcttca gttgccatag cctaccctcg
       61 gcttcgaaca aagcaaagcc ggcggactcc agatctgtaa tgtcgcacgg aatcggcggg
      121 ctctcccgcc cactgatcgc cgttgctatc gctcgtacta tttccaaatg tcgtgacttc
      181 actcgtacca acaagatcag tggaacaatc acgcgatgat tctcatgctg tgaagtggag
      241 gccgttccgt tgccccacac ctatccggcc ttaaaaacca ccgaactggt gctcacgtaa
      301 atactcgcca agcatcgttg cccctcctcc gtgccaacat agctgagaca ctcctcgtag
      361 tcggaattag agtgcagggc gagaaggata atcagcatgt tagaccagaa gatcaacggc
      421 cttccgtccc ctgcgacttt ggctacggcc cgacgcagtg agggcgagcg aagcgaggtc
      481 gaacggagtg gggccgcagc caaagccagc gtcgaatcca ttgcccgccc cgatcccgag
      541 gtggttgcca aagccaaacg tcgccgattc actggcgact acaagcgacg catactcatg
      601 gaagccgatg ccgcgaaggc gaccggcgac ctcgggtcgc tcttgcgtcg agaacatctc
      661 tactcgtcgc ttctggtgac gtggcgtcgc gaacgcgatg cgggaatcgt caaaggcttg
      721 actccctcca agcgcggtcc caagtccaag caaaatccgt tggacgccga agtgagtcac
      781 ttgcgtcgcc agaacgctcg cctgaccgaa gacctgcgca aggccgcgat cgtaatcgat
      841 gttcaaaaaa aactggcaac gctgctggag agcctagcca gtcaagaaac ggccgagaag
      901 cgcgcatgaa cgccgctctc cacttggctt ccacggttgg cgtccaatcc gcatgcgagg
      961 cgctcggcct ggtgcgcgcc tcgttctatc ggcaacgccc gctcctcggc ccgacggagc
     1021 aggccaatcc gctccgatcc gatgcgatcg aagccatcga accgaccgct tctcctcggg
     1081 cgttgagttc ctccgaacgc gagacagtgc tggcctgtct acacagcgaa cgttttcagg
     1141 atcgctcgcc ggcggccgtt tacgccacac tgctcgacga gggcgtctat cactgctccc
     1201 tgcgcacgat gtatcggctg ctggaagaag aaggcgagtc tggcgagcgg cgcgatcagc
     1261 tcactcatcc ggcctatcaa aagccggagt tgcttgccac cgaacccaat cagctgtgga
     1321 gctgggacat taccaagctg atgggcgccg ccaaatggac ctattactat ctgtacgtca
     1381 tcatggacgt ctatagccgc tacattgttg gttggatggt ggctccccgc gagaccgccg
     1441 agttggccaa gcggctgatc gaagatacct gtcacaagca gaacattggc agagaccagt
     1501 tgaccatcca cgccgaccgc ggttcttcga tgacttcaaa gccggttgcc tttttgctgg
     1561 ccgacttggg cgtcaccaag acgcatagcc gcccccacgt ctccgacgac aatccctatt
     1621 cggaaagcca gtttcgcacc atgaagtacc ggcccgagtt tccggaccgc tttggatcga
     1681 tccaagacag tcgagccttc tgccagacct tcttcgcctg gtacaacgac gagcatcgcc
     1741 attccggtct gggtcttctg accccggcca tggtccatca cggccaggct ccgctcatcc
     1801 ttgcacaacg ccagaacgtc ctcaatgccg ccttcttggc tcatccggaa cgattcgtcc
     1861 ggttcgctcc caagccctcg ccccttccaa aggaggtctg gatcaacaag ccacccgcgt
     1921 cagtaaacaa tactcagtaa attatgcttc cgagtgtctc aaatctattg acacgcgccg
     1981 cgcgtcaaag tatccaatgg tgctgtcaac gtcccgaaga taggttcgat ccgtatccgc
     2041 cagtcgcagt cggtggacgg cgagaccaag agcgccacga tcaagcaaga cgcctcgggt
     2101 aactgggacg tgtcgcgggt g
//
LOCUS       DASRKK010000007         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_586816, whole genome shotgun sequence.
ACCESSION   DASRKK010000007 DASRKK010000000
VERSION     DASRKK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586816"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1422)
                     /locus_tag="VFC15_00090"
     CDS             complement(373..1422)
                     /locus_tag="VFC15_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZM08593.1"
                     /translation="MRPAELAHQAGGSYRDGKPVITHEEKRAIGEGKGVLMMNQDSEL
                     DAFKREIDLRQFAVSLGYEIDRGESWRGSTVLRRGGDKIVVKRNGNGHYVFFSVRDDR
                     DNGTVIDLLQRRQNLSLGAVRQILRPWIGRPAISSHFPKLEPAGPDRMRVEGAYRRMA
                     DAHRFSYLELERYVPAAVLLLPRFAGRMRIDSLGNTVFPHFDAAGLCGYEIKNCGFTG
                     FAAGGQKGLWLSHTQPADRRLILTESAVDALSYGALFPDAEDQTRYASLGGKPSSRQR
                     ELVQATIAKLPAGAEIVAAFDADDAGRQMVEVIREAVASVAGRTGRSDLIFKAHLPTQ
                     EGEDWNMVLQNVGLV"
     gene            complement(1362..2117)
                     /gene="istB"
                     /locus_tag="VFC15_00095"
     CDS             complement(1362..2117)
                     /gene="istB"
                     /locus_tag="VFC15_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM08594.1"
                     /translation="MNLIELQRALRQLRLGATAAVLETRLRQAQAEAMAPIDLISCLV
                     SDELTRRSERLLDRRRKQAAFRDPHKTLDNFDFTFNPKMNRSLVFDLATCAFIGKRED
                     ALFLGPGGTGKSHLGQAIGQAAIQQNYRVMYRETHVLLDHLAEAVVEGTRKQFMESVA
                     SVPLLIIDDFGMRKLPHTAAEDLLEIIMRRYERASTLLTSNRPVEDWGKLLGDAAAVT
                     AMLDRLLHHGHVLKCGPRSWRTKLAAATATESQ"
     gene            complement(2114..>3416)
                     /gene="istA"
                     /locus_tag="VFC15_00100"
     CDS             complement(2114..>3416)
                     /gene="istA"
                     /locus_tag="VFC15_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM08595.1"
                     /translation="SACEPHRETIELELSKGRNAKGIWQDLVDRSGFAGGYQSVKRFI
                     VGLRGKSSPEACAVIETAPGEECQVDYGSGPMVRDSGSGKYRRTRLFVLTLGYSRKAV
                     RLLVFGSSSQIWAELHEKAFRRLGGVTKLVVLDNLKEGVLAPDIYDPTLNPLYRDVLS
                     HYGSVALACRVRDPDRKGKVESGVGHAQKTPLKGQRFETLDEAQAYLDHWEERWADTR
                     IHGTTKRQVAAMFAEEKSVLLPLPIEAFRYYQYGKRKVNLDGCVEVDAAYYSAPPGWI
                     GQEVPVQWDRRYVRLLHPRTGELLREHLPQVRGKHRIKDEDRPKHTPPGIQHLLLRAG
                     RAGQQIGVVCRKMYEEEGQVAVRRIQGVMAMAKKHGAAMVEDACAVALEAGASNYYRF
                     VRRYLERGPQLPLSLRQVDPLIRELTEYRDFIQSKTRENQS"
BASE COUNT          633 a         1087 c          966 g          730 t
ORIGIN      
        1 caacgacaga gcctaccgcg ctctctaccc agccttacga gttcacttca cgaatagtcg
       61 ttgatctgct gtcaaaattg taggctgtct ccatggtctc aatacacgtt tgtggaaaag
      121 tgactcccta gtctggacat aggcaaccga tctgaaaccg tctgctacca tctgaacgtg
      181 tcgcactcaa acaccatcgt cacctcgggg atcacttcaa aaccggccat agaggatcac
      241 ttcaaaaccg gccaacggac catcaccctg gacgaacttg ttttacctta ccggctggcg
      301 gtctggcaag tccagttttg accagttttg accagcccag tccctcatcg tcagcccctg
      361 agatgccggc tgtcatacca gcccgacgtt ctgcaggacc atattccagt cctccccttc
      421 ctgcgtcggg aggtgagctt tgaaaatcaa gtcgcttctt cccgttctac cggcgacact
      481 cgctaccgcc tcacgaatta cctccaccat ctggcgtcct gcatcatcgg cgtcgaatgc
      541 cgctacgatt tcggcccctg ccggtagctt cgcgatagtc gcttggacca gttccctttg
      601 ccgtgagctt ggtttgccac ccaagctggc atagcgggtc tggtcctccg catccggaaa
      661 cagggcgcca taggagagcg cgtccacggc gctctcggtc aagatcagac gccggtcggc
      721 cggctgggta tgcgataacc acaaaccctt ctggcccccg gcggcaaagc cggtgaatcc
      781 acaattcttg atctcgtatc cgcacaaccc cgcggcatcg aaatgtggaa ataccgtgtt
      841 gccgagggag tcgatccgca tgcggcccgc aaaccgcggc aataacaaca ctgccgcggg
      901 cacatagcgc tcgagctcca ggtacgaaaa ccggtgcgcg tctgccatgc gccggtacgc
      961 gccttcgaca cgcatccggt ccggcccggc cggctccagt ttgggaaagt gcgaggagat
     1021 cgcaggccgg ccgatccatg gccgcagaat ctgacgcacc gcaccgaggc tcagattctg
     1081 gcgcctttgc agaagatcga tgacggtgcc gttgtcccgg tcatctctca ctgaaaagaa
     1141 gacataatgg ccattaccat tgcgcttgac aacgatcttg tcgccgccgc gccgcagcac
     1201 cgtacttccg cgccaactct cgccccggtc gatttcgtag ccaaggctca cggcaaattg
     1261 gcgtagatcg atctcccgtt tgaaggcgtc gagttcgcta tcttgattca tcatcaaaac
     1321 accttttcct tcccctattg cccgtttttc ttcgtgggtg atcactggct ttccgtcgcg
     1381 gtagctgccg ccagcttggt gcgccagctc cgcgggccgc acttgagcac atgcccgtga
     1441 tgcagcaggc ggtccagcat ggcggtgacc gcggccgcat cgcccagcag ttttccccag
     1501 tcttccacgg gtctgttcga tgtcagcagc gtgctcgccc gctcgtatcg ccgcatgatg
     1561 atttccagca ggtcctcggc tgcggtatgc ggcagtttgc gcatgccgaa atcgtcgatg
     1621 atgaggagcg gcacgctggc caccgactcc atgaactgct tgcgcgtacc ctcaactacc
     1681 gcctccgcca gatgatccag cagcacatgg gtttcccggt acattacccg gtagttctgt
     1741 tggatagcgg cttgcccgat ggcttggccc aagtgactct ttcccgtccc accgggaccc
     1801 aggaaaagtg catcttcccg cttgccgatg aaggcacaag tggccaggtc aaacaccagg
     1861 ctgcgattca tcttcgggtt gaaagtgaag tcgaagttgt ccagagtctt gtgcgggtcg
     1921 cggaaggcgg cctgtttgcg ccggcgatcc aagagtctct cgctgcggcg ggtaagctcg
     1981 tcactcacca gacacgagat cagatcgatc ggcgccatgg cttcggcctg tgcttggcgc
     2041 agtctggttt ccagcacggc ggcggtggcg ccgagccgga gttgtcttaa agcacgttgt
     2101 aattcaatca ggttcatgat tggttctccc gggttttgct ttgaatgaaa tcgcggtatt
     2161 cggtgagttc gcggatgagc ggatccactt ggcgcaggct cagcggcagt tgcggccccc
     2221 gttccagata gcggcgcaca aaacggtagt agttggaagc tccggcctcc agagccacgg
     2281 cgcaggcatc ttccaccatc gctgcgccat gcttcttggc catcgccatc acgccctgga
     2341 tgcggcgcac ggcgacttgt ccctcttcct cgtacatctt gcggcacacg actccaatct
     2401 gctgaccggc gcggccggcg cgcagcagta ggtgttgtat ccccggcggt gtgtgtttgg
     2461 ggcggtcctc gtccttgatc cgatgcttgc cgcgcacctg aggcaggtgt tcccgcaaca
     2521 gttcgccggt ccgcgggtgc agcaagcgga catagcggcg atcccattgc accggcacct
     2581 cctggccgat ccaccctggc ggcgcgctgt agtaggcggc atcgacttcc acgcagccat
     2641 ccagattgac cttgcgcttg ccatattggt agtagcggaa agcttcgatc ggcaatggaa
     2701 gcaagacgga tttttcttcg gcgaacatgg ccgccacttg ccgtttggta gtgccgtgaa
     2761 tgcgggtgtc ggcccagcgt tcctcccagt ggtccaggta ggcctgagct tcgtccagag
     2821 tttcgaagcg ctgtcccttg aggggcgttt tttgagcatg gccgacgcct gactcgacct
     2881 tccccttgcg gtccggatcg cgcacgcggc aggccagcgc caccgagccg tagtgggaca
     2941 gcacgtcacg gtagagaggg ttgagcgtgg gatcgtagat gtcgggggcc aggacgccct
     3001 ctttgaggtt atccagcacc accaacttgg tgacgccccc cagtcggcga aaagcttttt
     3061 cgtgcaactc cgcccagatc tgggaactgg aaccgaacac cagcagtcgc accgctttgc
     3121 ggctgtaacc cagcgtcagc acaaacaggc gggtacggcg atatttgccg ctgccggagt
     3181 cgcgcaccat ggggcccgat ccgtaatcca cctggcattc ttccccgggt gcagtttcga
     3241 tcaccgcgca ggcttctggc gacgacttgc cacggagacc aacgatgaac cgcttgacac
     3301 tttgatagcc accggcgaag ccgctgcgat ccaccaggtc ctgccagata cctttggcgt
     3361 tgcggccctt cgagagttcc aactcgatgg tttcccggtg tggctcgcag gcacta
//
LOCUS       DASRKK010000008         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1173207, whole genome shotgun sequence.
ACCESSION   DASRKK010000008 DASRKK010000000
VERSION     DASRKK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173207"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFC15_00105"
     CDS             complement(<1..1145)
                     /locus_tag="VFC15_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM08596.1"
                     /translation="MKIFRGMVPVALLAGLVFGTGCETDHPKPAATSLPTQAKAPEIA
                     DPVAQAADPKPKVQPDVTTKPDPAEALIAQAEKQYAAGQANYQADHLEAAKQNFDQAF
                     NTLLSSNLDIRNDERLEREFDKIVEAVHELEMAALKQGDGFSEPRSEPAPIDEANDIT
                     FPVDPNIKAKAEAEMKTTKSDLPLVLNDQVAMFINYYSSPRGRATLERAWARSGRYRE
                     MVSRIFKEEGVPQDLIYLAQAESGFQPLALSRAGARGMWQFMAASGELYGLQRSWWVD
                     DRQDPEKATRAAARNLKDLYKQFGDWYLAMAAYNSGASTVQHAVERTGYADFWELYRR
                     GVLPQETRNYVPIILAETIMAKNPEQYGLQRVAPDPAPQMEQVTINYP"
     gene            complement(1294..2496)
                     /locus_tag="VFC15_00110"
     CDS             complement(1294..2496)
                     /locus_tag="VFC15_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM08597.1"
                     /translation="MLSKGLWMNRKASADATRCVHSGEDRHGQAAPLTTPIAQTAVFV
                     LPSVKALRHYAEGNRDLYLYSRYGNPTVAAAEEKIAALEGAEAAVATSSGMAAELVAA
                     LITCQAGDEIVSMLDVYGGTLKLFEQVLPCCGIKTRFIPYHDIGNAHRYFSRKTRMLF
                     LETPTNPTVRCVDLAALAEVGRKHKSCVVVDNTFATPVLQKPLELGADIVIHSATKYL
                     GGHSDLTAGVLAGSKKWMDAARQTMILTGGCLDPGCAYLLLRGLKTLHVRVERACRNA
                     AQIAEALEAHPKVARVYYPGLESHEAHELARRQMKDFGMIVSFDLRGGERAAERFIDR
                     LQLWYLATSLGGVESTVSYPVLSSHVGLGAKRLDLLGVSPSTIRLSVGIEDAGDLIAD
                     LNQALTHA"
BASE COUNT          513 a          878 c          870 g          596 t
ORIGIN      
        1 gggtagttga tggtcacctg ctccatctgc ggcgcggggt caggggccac gcgctgcagc
       61 ccatactgct ccggattctt ggccataatg gtctcggcca ggatgatggg cacatagtta
      121 cgggtctcct ggggcagcac gccacgccgg tacagttccc agaaatcggc atagccggtg
      181 cgttccaccg cgtgctgcac ggtgctggcg cctgagttgt aggccgccat tgccaggtac
      241 caatcgccga actgcttgta gaggtccttc aagttgcggg ccgcggcacg ggtcgctttt
      301 tctggatcct ggcgatcatc cacccaccag ctccgttgca ggccgtacaa ctcgcccgac
      361 gcggccatga actgccacat gccgcgcgcg cccgcccgtg aaagcgccag gggttgaaat
      421 cccgactccg cctgcgccag gtagattagg tcctgaggaa cgccctcctc cttgaaaatc
      481 cgcgacacca tctcccggta acggcctgac ctggcccatg cgcgctccag cgtggcccgt
      541 cctcgcgggc tggagtagta gttgataaac attgccacct ggtcgttgag caccagcggc
      601 aagtccgact tggtggtctt catctcggct tcggccttgg ccttgatgtt gggatcgacg
      661 ggaaaggtga tgtcgttggc ttcgtcgata ggcgccggtt cggagcgcgg ctcgctgaag
      721 ccgtctccct gcttcagcgc cgccatttca agttcgtgga cggcctcgac gattttgtcg
      781 aattcgcgct ccagccgttc atcgttgcgg atgtcgagat tgctggaaag cagggtgttg
      841 aacgcctggt cgaagttctg cttggcggct tccagatggt ccgcctgata attggcctgt
      901 ccggctgcgt attgcttttc cgcctgcgca atcagcgcct cggccggatc gggcttcgta
      961 gtcacatctg gctggacttt gggtttagga tcagccgctt gggcgacggg atcggcgatc
     1021 tcgggagctt tggcttgagt tggaagggaa gtggcggccg gtttggggtg atcggtctcg
     1081 catccggtgc caaacaccag acctgcgagg agggccaccg gaaccatacc gcgaaagatt
     1141 ttcattactt cgaaacagcc ctctctcact ccactctcgg ccagtgaatg tgatgttact
     1201 atgcggcgaa attcagggtc aacggaagcc aaagcctgtc agtaactgcc gcttgcacaa
     1261 tggtttgacg gctgaggcgc caacaggttt ggctcaggcg tgggtcagtg cctgattcaa
     1321 atcagcgatc aagtccccgg cgtcctcgat tccaacggaa agccgaatgg ttgacggcga
     1381 cacgccaagc aagtctagtc gcttggcgcc tagtccgacg tgcgacgaca gcactgggta
     1441 cgatactgtc gactccacgc ctcccaggct ggttgccaga taccagagtt gcaggcggtc
     1501 aatgaagcgc tcagccgcgc gttcgccccc gcgaaggtcg aacgacacta tcatcccgaa
     1561 gtctttcatc tggcggcgcg ccagttcatg cgcctcgtga ctttccaagc cgggatagta
     1621 cacgcgcgcc actttgggat gggcttcgag cgcctcggca atttgtgcgg cgttgcggca
     1681 ggcgcgttcc actcgcacgt gcagcgtctt caggccgcgc agcagcaggt aggcgcagcc
     1741 cggatcgagg catccgccgg tgaggatcat cgtctggcgg gcggcatcca tccacttctt
     1801 cgaaccggcc agcacgccgg cggtgagatc gctgtgaccg cccaggtatt tggtcgccga
     1861 gtggatgacg atgtcggcgc ccagctccag cggcttttgc agcactggag tggcgaaggt
     1921 gttgtcgacc accacgcacg acttgtgctt acggcccact tcggccagcg cagccagatc
     1981 gacgcagcgc accgtcggat tggttggggt ctccaggaag agcattcgcg tcttgcggct
     2041 gaaatagcga tgagcattcc cgatatcgtg atacggaatg aagcgcgtct tgatgccgca
     2101 gcacggcagc acctgctcga acagcttcag cgttccgcca tagacgtcga gcatggaaac
     2161 gatctcgtcg ccggcctggc aggttatcag ggccgcgacc agctccgccg ccatcccgct
     2221 ggaggtggcg actgcggcct ctgctccttc cagcgcggca atcttctctt cggccgccgc
     2281 gaccgtcggg ttgccgtaac gcgaatagag atacaggtcg cgattgccct cggcatagtg
     2341 gcgcagcgct ttgacgctgg gaagcacgaa cacggcggtt tgcgcgatag gcgtggtcaa
     2401 gggcgccgct tggccgtgcc gatcttcgcc ggaatgcaca cagcgcgtgg cgtcggcgct
     2461 ggctttcctg ttcatccaca atcctttcga gagcatttct cccgcacggg aaattcgatt
     2521 gtaattgaga ccctacctca gcagatgccg gcgtgttcgc cgattagaac cgggcccgcg
     2581 atgtggagaa aatccctcgt accctgcgtg cccgccgcat ccttctctac tcgcaatgcg
     2641 gactgagccg catccatcca gaacgatctt aacttgtcgc aaaagccagt cctaaggcgt
     2701 cgatagaata taatgtcgca cactgatggc cctcgcctcc ggcaccaaac ttggtcctta
     2761 tgaaatcgtc tcgccgctgg gcgctggcgg catgggagag gtgtaccttg cgcgcgatac
     2821 acgcctcaac cgtagcgtcg cggtcaaaat tcttccc
//
LOCUS       DASRKK010000009         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_489102, whole genome shotgun sequence.
ACCESSION   DASRKK010000009 DASRKK010000000
VERSION     DASRKK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489102"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFC15_00115"
     CDS             <1..455
                     /locus_tag="VFC15_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08598.1"
                     /translation="FIRTHMDVLVGTDFFTVEVLTLQGLVTYYVLFFIQLESRRVCLA
                     GITPHPDQQWMEQMGRNVTMQKWGFLANSRYLLHDRDSKFCLSFRQLIEAGRVKTMPL
                     PARSPNLNAYAERWVRSVKQECLSKLILFGERPLRRALQHSPPPKVVP"
     gene            complement(852..1283)
                     /locus_tag="VFC15_00120"
     CDS             complement(852..1283)
                     /locus_tag="VFC15_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08599.1"
                     /translation="MKHNTYGALTMLAVALMISVPTLQAQSRVRADVPFAFSLEQTSM
                     PAGGYEVSSIEDKVLAVRNLGTGQARLLIASMHVEVSQASGTPHAKLVFNKYGDQYFL
                     SQIWDGQSQSGIALSESKREKELKMASTNLSGPETVIIAMK"
     gene            complement(1780..1959)
                     /locus_tag="VFC15_00125"
     CDS             complement(1780..1959)
                     /locus_tag="VFC15_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HZM08600.1"
                     /translation="MCGRYHLSRRKQLIQEYFNSTDEVDWEPRYNIAPSQNVGIIRQD
                     GAKARAPFLNSRAGA"
     gene            2176..2460
                     /locus_tag="VFC15_00130"
     CDS             2176..2460
                     /locus_tag="VFC15_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08601.1"
                     /translation="MGNPGQHRTRFFGPSRRAATTALGFAIVFVLSVVATQAAQAQTF
                     KVLHTFTGVADGDTPAAGLTMDRAGDLYGTTQNGGDGRLMYRKIKISCSA"
     gene            complement(2589..3692)
                     /locus_tag="VFC15_00135"
     CDS             complement(2589..3692)
                     /locus_tag="VFC15_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08602.1"
                     /translation="MKYHHPLKKILSQARPYWDRDEMRPSVRWAFGKAWQCRTPELGA
                     EVYASEKGERTFYHTCKSRPCSSCGYRAVVQWQRGWWAALPDALYKGITFTMPDVLWS
                     LFRDNPLVAKALPALAAEVIKARISAKYGLRVGVIAILHTFNGKLEFNSHVHTMVTGG
                     GLYGSSDTWVSRAYHERDLLMEAWRRAVIALIRAALRAGRLRTKMTADQIEEMLTEQE
                     NRSWSIKIQSFKSKEHFLRYAGRYVRRPPIAQRRITYIGKQTVRFWYKDKKLRRRVYV
                     QCSLEEFIDRWAQHIPERYQHAVRSFGLFGPRALRQTSAAIFAILGQQRRLRPKPRRW
                     ADSLWRDFRHDPLLDQTGNRMKWVRRLPAKVSR"
     gene            complement(3689..>3952)
                     /locus_tag="VFC15_00140"
     CDS             complement(3689..>3952)
                     /locus_tag="VFC15_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08603.1"
                     /translation="PRAEQLEQFIRYSEERATNKVIRESIARPKDEKISPDVVTVLDA
                     LQQQEARQPGSVAVRVGRHKRTVVLIGQDLFAGPRSQKELKLT"
BASE COUNT          825 a         1090 c         1144 g          893 t
ORIGIN      
        1 atttcatccg cactcatatg gatgttctgg tgggaacgga tttctttacg gtggaagtgc
       61 ttacgctcca aggcttggtt acctactacg tgttgttctt tatccagttg gagagtcgga
      121 gggtttgtct ggccggaatc acaccccatc cggaccagca gtggatggaa cagatggggc
      181 gcaacgtaac gatgcagaag tggggatttc tggccaacag tagatatcta ctgcatgatc
      241 gggacagcaa gttctgcctc tcgttccgtc aactgatcga ggctggcagg gtgaaaacca
      301 tgcccctacc agctaggagt ccgaatttga acgcctatgc ggaacgctgg gtgagatcgg
      361 tgaaacagga atgcctgtcg aagctcatct tgttcgggga gaggccgctg cgccgagccc
      421 tgcagcattc accgccgccg aaggtcgttc catagagatt gcccccttta tcgatggtaa
      481 cgccagcctc cggataagcc ccgtcccccc caccagtgaa gttgtaaatg accttgtagg
      541 tctgtgcttg cgccgcttgg gttgggacga cggtgagtgc actcaggaat agcagggaga
      601 gtaccagagg agcagggacg aaacggcgca tggggcgctc ctttcttgca tgaggggcac
      661 gaagctgagt tagattggcc tgtggccgct agtctgttcc cgcgcctagg cgctgtcaat
      721 acgtttgcaa gtcggtaact agcacgtcag caggaaagat aaaaatcacc gagcgcggtg
      781 gtaaatccac gagagacgga acgacccgga gttctccact ccgggtcgtt tgctggccgt
      841 gaggttgcaa tctacttcat tgcgatgatc acggtctccg ggccagagag attggtgcta
      901 gccattttga gttccttctc gcgcttcgat tcggacaagg caattccact ctggctctgt
      961 ccgtcccaaa tctgcgaaag gaagtactgg tcgccgtact tgttgaatac cagcttcgca
     1021 tgcggagtgc cggaagcttg ggagacttcc acatgcatag acgcaatcag caagcgcgct
     1081 tggcccgtcc ccaggttccg aaccgccagg accttgtcct cgatggagct gacctcataa
     1141 ccgccagcgg gcatggaagt ttgttccagg ctgaaagcaa agggtacatc tgccctgacc
     1201 ctgctctgtg cctgaagcgt tggtacgctg atcatgagag ccaccgcgag catcgtgagg
     1261 gccccgtaag tgttgtgctt cataactcct ccttgggggc tcctgtggga acccagattg
     1321 aatttagttg gctgcgagac ttcgttggcc tttcttgcct ttggagcctt gctgcgaacg
     1381 cagacagtag tgcacgaggc gtgccaattc aaagttgaga aagcgcgatt ttcatttccc
     1441 tgtatttgca atggcatacc gcaatggcga ccatgtgaaa ctgtatggtc tcttccattt
     1501 ccgcccgaag aggctatccg gatggatatg gtcgactatc cattctgtta gccgatgact
     1561 gcctttcagc ctgcgccagc attatttgcc gcctgcgacg tcgccgcact ccggatcatc
     1621 gttctttacg aagtcacgtg cggcctcacc gggtagcgtt tcatcagccc tgcaaggggc
     1681 tccgcagctc cttgagcgcc tccgccttgc tggcgaccat ctccaagcgc gccttgatta
     1741 gcttctagcc gatgctgggg tccttcgccc aatacgggat taggccccag cgcgcgagtt
     1801 gagaaatggc gctcgggctt tagccccgtc ctgccgaatg ataccgacgt tctgcgacgg
     1861 ggcgatgttg taccgcggct cccaatcgac ctcgtcggtg gagttgaagt attcctggat
     1921 cagttgcttg cgccgcgata ggtgatagcg gccacacatg acgagactcc ggagtgccta
     1981 ttttaaagct tgctttgcgg aacgaacgtg cgcacaatct tgtcgttatt tggccgaaat
     2041 ggaagcgttc ggtcacacga gttggccgat cagggatgtc tggcacaacg cagatagacg
     2101 tgcagttccc accttcatga ttgagatagt ccctccccct gcactcaacc ttcgctttga
     2161 aaaaggagaa tactcatggg gaacccagga caacatcgga ctcggttttt cgggcccagc
     2221 cgacgagcgg caacaaccgc gctggggttc gcgattgtgt ttgtactgag cgtcgtcgca
     2281 acccaggcgg cgcaggccca gaccttcaaa gtgctgcaca ccttcactgg cgtggcggat
     2341 ggggatactc ctgcggccgg gttgacgatg gatcgagcag gagacctcta cggcacaacc
     2401 cagaatggcg gtgacggacg cttgatgtat aggaaaatca aaatctcgtg ttcggcgtga
     2461 acgaggcatt tctggcgggt cgagcgtgta ttgttggttg atggtgacga gcggaaagag
     2521 gacgcaagcg ggcgttctcc atcgcgcgat tgcgcaagga acgttgagca ttctcggcta
     2581 gattctgatt agcgggacac ctttgcaggt agtcgtcgca cccatttcat cctattgccg
     2641 gtttggtcga ggagcggatc gtgtctgaag tctcgccaga gggaatctgc ccaacggcga
     2701 ggtttggggc gtagccttcg ctgttgtccc aaaattgcaa agatagctgc cgaggtttgc
     2761 ctgagagcgc gcgggccaaa aagtccaaag ctgcgaacag cgtgctgata gcgttccgga
     2821 atgtgttgcg cccaacgatc aatgaactct tccagggaac actgtacgta aacccggcga
     2881 cgcagcttct tgtctttgta ccaaaacctg acggtctgct tcccaatgta ggtgatgcgg
     2941 cgctgcgcaa tgggtggccg ccttacgtac cgaccggcgt atcgcaggaa atgctctttc
     3001 gacttgaagg attgaatctt gatgctccac gaacgattct cctgctcggt aagcatctct
     3061 tctatctgat cagcggtcat cttcgtgcga aggcggcccg ctcgtagggc tgcgcggata
     3121 agcgcgatca cggccctccg ccaagcctcc atcagtaggt cccgctcatg gtaggcgcga
     3181 gaaacccagg tgtcggaaga cccatacagc cctcccccgg taaccatggt gtgtacatgt
     3241 gagttgaact ctaacttccc gttgaaggta tggaggatgg caatgacgcc aacccgcaag
     3301 ccatatttcg cactaatccg cgcctttatg acctccgcgg ctagagctgg cagagcttta
     3361 gccacgagcg gattgtcgcg gaaaagggac cagaggacat ccggcatcgt gaaggtgatc
     3421 cccttataaa gcgcatctgg cagtgcggcc caccacccac gctgccactg cacaacggcg
     3481 cggtagccgc aactggagca gggtcgtgat ttgcaggtgt gatagaaagt tcgctctcct
     3541 ttctccgagg cgtaaacttc ggcgcctaac tcgggagtac ggcactgcca cgccttacca
     3601 aaggcccagc gaaccgaggg gcgcatctca tcccggtccc agtacggacg cgcctgagac
     3661 agaatcttct tgagcggatg atggtatttc atgtcagttt caactccttt tgagaacgcg
     3721 gtccggcgaa cagatcttga ccgataagaa caacagtgcg cttatgccgg ccgacccgta
     3781 ccgcaaccga tccaggctgt cgcgcctcct gctgttgtaa ggcgtcgagt acggtgacaa
     3841 cgtcggggct gatcttttca tctttgggcc gagcgataga ctcacggatc accttgttcg
     3901 ttgcgcgttc ctcgctgtaa cggatgaact gctccaactg ctcggcccgg gg
//
LOCUS       DASRKK010000010         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1075479, whole genome shotgun sequence.
ACCESSION   DASRKK010000010 DASRKK010000000
VERSION     DASRKK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075479"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFC15_00145"
     CDS             complement(<1..387)
                     /locus_tag="VFC15_00145"
                     /inference="COORDINATES: protein motif:HMM:NF026041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZM08604.1"
                     /translation="MRENNWTKLLGWPGYRVYRHEIDEHGKTLKLWVRRKRGNRKLVC
                     SGCGRKLTEACDIYQREVRDLPWSEYRATVVIELYRVRCPDCGVKTEKVPQLPSKAPF
                     SQRFEEAVGLACESAAVRRVAQQFGLA"
     gene            2249..>2848
                     /locus_tag="VFC15_00150"
     CDS             2249..>2848
                     /locus_tag="VFC15_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08605.1"
                     /translation="MSPQEAIPVKIPISTSRETYIAIVTALAIASHLILKYLTHLPRL
                     AYLIPLFVALAVGGIPILVTLGKKLWVREFGSDLLAGISILASVLMGQYLVGAIIVLM
                     LSGGAALEQFATRRASSVLDALAKRMPQIAHCKRGTSVEDVNLDQVHIGDELIVLPHE
                     ICPVDGLVVEGHGVMDESYLTGEPFEISKTPGSRVLSGAI"
BASE COUNT          614 a          801 c          810 g          623 t
ORIGIN      
        1 tgccagccca aactgctgcg ccacccgccg caccgccgca ctctcgcagg ccagccctac
       61 cgcctcttcg aagcgctggc tgaacggagc cttgctgggc aattgcggca ctttctccgt
      121 cttcaccccg cagtccgggc aacgcactcg atacaactca atcaccaccg tcgcccgata
      181 ctccgaccag ggcagatcgc gcacctcgcg ctgatagatg tcgcaggctt cggtcaactt
      241 ccgtccacag ccagaacaca ccagcttccg gttgccgcgc ttgcgccgca cccacaactt
      301 cagagttttg ccatgctcgt cgatttcgtg ccggtacacg cggtagcccg gccaccccag
      361 cagtttggtc cagttgttct ctcgcacccg actgagtgta cccagaactc cccaaaatca
      421 aaatgcccca tgaaatcagg ggctgttcag attcctgctc agaacccttt tcattgaaag
      481 tttcctttcc tctttggttg aaatttgggg cccggcgcat ctagcgacga gccctggtgt
      541 ttaagcgtga gattggcgat attgttggcg ctcagatact gatcaatcgc aaagggtatg
      601 gaaaacgtgt ccattcaaaa ttgacccaag ctggattctt gtggcctgct cccctcgtgg
      661 tatcgatcca cgcttgtcag agcacctgat gtgccaaacc gtcaccctat cgcgagtgct
      721 caaagtgatg cacttacggc ggaagaggag ggcgggtaat tgcggcgagg tacaagtttt
      781 tgaacaactc cctaaacctg tcaaaaagtt cgaacgcgga gatcgcaagc tggaattgtc
      841 gcgggctggc gtatgtcgtt gtaacgcgcg gggaaaagcg ccaatatggc gcgccggtac
      901 ctgtaaccaa taaggacgga gaaggtggaa ggcgatttgc tctgacctca ggctaggagc
      961 actgaggtcg caatcaagcg tgacgcagcc gttacaggga gcgcctggca agttcgagtc
     1021 aatgcaaagg ggtcaacaga cccataggcg gtctgacttc gatcggaaaa caggtggcgc
     1081 aggaagccga gcagccgaca agaccagcta agggggcaca tgcaggttga gattcgtgga
     1141 aagggtcttc gagtaaccga tgttctgagc acatacatcc aacgccggct cgtctttgcg
     1201 ttggggcgat tcggtcgacg ggtagagaga gtgttggttt gggtcgaaga tacaaacgac
     1261 ccgaagggtg ggatcgacaa acagtgccgt gttgccgtcg tggtgcctca ctccagaacc
     1321 gccgtgatag aagccagagg ctcgaacatt cgcgtgacca tcaatcgtgc cgttgccaag
     1381 gcgagcaggt atatcgcaga ccggctgaag cagccccatt cgaccaactt gtcggcccgc
     1441 cagagccggg cttcggagaa tcgcataact ccttactgtt gaggtccccc catgacgccg
     1501 cccaactcgc ttaatgcttg tcctcttgag ctgaaagtag cggatgaagc ggcatggtct
     1561 caactggtct gtcacctgaa atcagcgcat gccgaggaac gttatattgc gttggaagaa
     1621 gccgccgatc tgcttggagc tgctatcgcc agaggcgagc tggacggcga gtcggcgaga
     1681 aggtggatga tcgccttatt cactgcggtc gctgccattg gaggtgggat cccccgggct
     1741 tccgcttcac ccttcgcctg cgattgttga gaggagtaat cgatgctttc caagtatgca
     1801 aaccctcgat gttccgcccc atttcttacc ctgagaaagg ggcggctggt cgtacttcat
     1861 ttgcgccagc cggtactaaa agggaccttt gccgaagccg aagccgacgt caaagatttc
     1921 gaaccctttt ggttgtgtga ggagtgctgt gatcgcttca cgctccttgt gaagccgggc
     1981 agagagtgtg tttgcatccc gaggccgccg agcacgggcg actacactcg gagcgagcca
     2041 ttgaccatcg gctcggaact ctgactggcg gcggtgctcc cggtgtcaag gaatcactgc
     2101 ctattttagc tgtgcgtgtt caggtaacct ccgtcacagc ctacaaggtc gttggagcgc
     2161 tactgtaaca ggcagctttg acgcggcccg agtcgaccgg ttggtggaga atctcagaag
     2221 ccgaacgtcg caaagtgatg ttcatttcat gtccccacaa gaagcgattc ccgtcaaaat
     2281 accgatctcc acttcccgcg agacatacat cgcgatagta accgcgcttg cgatcgcttc
     2341 acacctgatt ctcaaatatc tgacccatct gccccgattg gcctatttaa tccctctatt
     2401 cgtcgctctg gcggtgggcg gaattccaat tctcgtgacg ctaggcaaga aactctgggt
     2461 aagggagttc ggttcggact tgctggccgg aatctccatt ttggcctccg ttctgatggg
     2521 acagtacctt gtgggagcga ttatcgttct gatgctttcg ggcggagccg cgttggaaca
     2581 gttcgcaaca cgcagggcgt cctccgttct ggacgcgttg gccaagcgca tgccgcaaat
     2641 tgcgcactgc aaacgcggaa catcggtcga ggacgtaaac ctcgatcaag ttcacatcgg
     2701 cgatgaactc atcgtgttac cccacgaaat ctgtccggtt gatggactgg tggtggaggg
     2761 acatggggtg atggacgagt cttatctgac aggcgagccg tttgagatct caaagactcc
     2821 gggctcgagg gtcctctctg gagcgatc
//
LOCUS       DASRKK010000011         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_977878, whole genome shotgun sequence.
ACCESSION   DASRKK010000011 DASRKK010000000
VERSION     DASRKK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977878"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFC15_00155"
     CDS             complement(<1..1195)
                     /locus_tag="VFC15_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZM08606.1"
                     /translation="MFKNLSPLFPYLRKYRVTFWIGALCVLCNNGVWILFPLVIRRAI
                     DDLNLGVTRHKLFTYSALLLAVAGTKAIFQFLTRWILIGVSREIEFDLRNDLFTHLER
                     LSYSFYQRTRTGDIMARATNDLNAVRMLAGPGIMYTANTVVFTAGALVFMLSISPRLT
                     LFAFLPLPVVSIVVQYFGRKIHERFERIQAMFSDISAKAQENFSGARLIRAYVQEQAE
                     IESFERENTEYIARSLKLVRLMGMLWPTLETLLGLAVVIVLWIGGREVLLHRISVGSF
                     VAFNTYMVQLTWPIISLGWVINIFQRGTASMGRIQNLLAEQPEVTDAAVPATGAPTEV
                     RGGIEFRNLSFHYGHTLVAAERGMGHVHGDEAVLKNINLRVPEGTSLAIVGPTGSGKS
                     TLVSLI"
     gene            complement(1471..>2047)
                     /locus_tag="VFC15_00160"
     CDS             complement(1471..>2047)
                     /locus_tag="VFC15_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZM08607.1"
                     /translation="LGGVSIFEFDPNTFQLTRRIYAAHAHWGESLNKWVFEQGWIRTL
                     SGTSIQDYRTFDVATFNELREDPSYFKKEVKQSSEMNYEELQRYIGDLEQSGFDTVRL
                     KVQLQKKLAFPLITLVMAVLAVPFAASGRKGGALGGVVIAIAIAVVYWVTAGLFEAMG
                     NANQLPAMLAAWAPDFIFALAGGYFLLRVPT"
BASE COUNT          448 a          595 c          604 g          400 t
ORIGIN      
        1 gaatgagcga caccagcgtg gacttgcccg atccggtggg gcctacaatc gccaggctgg
       61 tgccttcggg cacccgcagg ttgatgtttt tcagcaccgc ttcatcgcca tgaacatggc
      121 ccatgccgcg ctcggccgcg accaacgtat gaccgtagtg gaagctgagg ttgcgaaact
      181 cgattccgcc gcgaacctcg gtcggagcgc cggttgcggg cacagcggcg tcggtgacct
      241 cgggctgctc cgccaggaga ttttggatgc ggcccattga cgcggtgccg cgctggaaga
      301 tgttgatgac ccagcccaac gaaatgattg gccaagtgag ctgcaccatg taggtattga
      361 aggccacgaa gctaccgaca gaaatgcgat gcagcaggac ttcgcggccg ccgatccaca
      421 gtacgattac gacggcgagg ccgagcagcg tttccagcgt aggccacagc atccccatca
      481 gccggaccag cttgaggctg cgcgcgatgt attccgtgtt ctcgcgctcg aagctctcga
      541 tctccgcttg ctcctgcacg taagcgcgaa tcagccgcgc gccggaaaag ttctcctgcg
      601 ccttggccga aatgtcggag aacattgcct ggatgcgctc gaagcgttcg tgaatcttgc
      661 ggccgaagta ctgcaccacg atgctcacga ccggcaaggg caggaaagcg aacagggtta
      721 gccgggggct gatgctcagc ataaacacca gcgcgcccgc ggtgaagacc acggtgttgg
      781 cggtgtacat gatgcccggc ccggccagca tgcgcaccgc gttgaggtcg ttggtagccc
      841 gggccatgat gtcaccggtg cgcgtgcgct gataaaagct gtacgacagg cgctccagat
      901 gtgtgaacag atcgttgcgc agatcgaatt caatttcgcg cgagaccccg atcaagatcc
      961 agcgggtaag gaactgaaag atggctttgg ttcccgccac cgccaacagc agcgccgagt
     1021 aagtgaatag cttgtggcgt gtgactccga ggttgaggtc gtcaatggcg cggcggatga
     1081 ccagcgggaa gagaatccag accccgttgt tgcacagcac gcatagagcg ccgatccaga
     1141 aggtcactcg gtacttgcgc aggtagggaa agagcgggct taggttcttg aacatgagtc
     1201 gacagaaatt aggtgtttgt ccttggactt tagctcaaag gtcttacacc tcgcgaaaaa
     1261 gtctggaaag cagatccctc ttcgtcaatt gagtgctgca cgattgatgg ccgcttcata
     1321 gcttcggagt tgtcatcacg aacggccttt aggccgtgag ggatctgctg tttttcccag
     1381 ttatcgttcc accgcggaca acagcagatt cctcgcttcg ctcgtaatga caattccaaa
     1441 cgcaaacaat ttccgcaatc tgcaagaacg ctacgtcggc actcgcaaca gaaagtagcc
     1501 tccggcaagg gcaaagataa agtcgggcgc ccaggccgcc agcatggcgg gcagttgatt
     1561 tgcgttgccc atggcctcga acagccccgc ggtcacccaa tacaccacgg cgatggcgat
     1621 cgcaatcacc actccaccca gcgcgcctcc cttgcggccg gaagctgcaa agggaaccgc
     1681 cagcaccgcc atcaccagag tgatcaaggg aaaggcgagt ttcttctgca actgcacctt
     1741 cagacgcacg gtgtcaaaac cactctgctc caggtcgccg atgtagcgct gcagctcttc
     1801 ataattcatt tccgatgact gcttgacttc tttcttgaag tagctcgggt cctcgcgcag
     1861 ttcattgaaa gtcgcgacat cgaaggtacg gtaatcctgg atggaggtgc cggacagggt
     1921 ccgtatccag ccctgctcga agacccactt gttcaggctc tctccccaat gcgcgtgggc
     1981 ggcgtagatg cggcgcgtga gttgaaaggt attcgggtca aactcgaaga tcgagactcc
     2041 cccgagc
//
LOCUS       DASRKK010000012         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_489273, whole genome shotgun sequence.
ACCESSION   DASRKK010000012 DASRKK010000000
VERSION     DASRKK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489273"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFC15_00165"
     CDS             <1..1275
                     /locus_tag="VFC15_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08608.1"
                     /translation="VETEIAPAEATPPPSTDDVQERLGLEVDSEAEERNDRPRYLDLN
                     EPSTPPKPAVAAMPIAVPSFLGLSDVPQVEAETGNELGVEEPARGRWRIWLAAAVILI
                     FGFLGALEWRAQVRQTNNGPVEVIKMKMWNLTHNKPAKSASAESASPATPSASASKPE
                     MQVEPPSNPPKQNPAASTATSAAAKNAANVPNTTAATAPLASSVRATPAPTGNQPVAA
                     QPNVTPPQNPALNATRTPAGQNTVATKTATTMPPMDKSKLIGSRQPGSAAVSTADKAK
                     PSPRAADDSEEAVAKRIIPGAEEMAKANSASDSAAEAAWLWKAIARGNPSAPVRLADM
                     YVKGDGVPRSCEQAVVLLKTAAEKENALARKRLASMYGSGTCVQRNRVEAYRWLSSAL
                     VADPNSEWAQQHRNLLWQQMTPEERTSAEKYR"
     gene            1445..>2005
                     /locus_tag="VFC15_00170"
     CDS             1445..>2005
                     /locus_tag="VFC15_00170"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZM08609.1"
                     /translation="MAHEVPALPYDYSALEPYIDTQTMHLHHDKHHVAYVTNLNAALE
                     KAPELQKLSAEELVKSLANVPEAIRTAVRNNAGGHVNHSMFWTIMGPNAGGEPSGAIA
                     DAIKNTFGDFNSFKEKFNDAGLKQFGSGWTWLVRDRGGKLLVMATPNQDSPLSQDFSP
                     IMGNDVWEHAYYLKYQNRRADYLKEWW"
BASE COUNT          462 a          624 c          591 g          328 t
ORIGIN      
        1 gtggagacgg agatcgcccc agccgaagcg acgcctcctc cctccacgga tgatgtgcaa
       61 gagcggctgg gattggaagt cgatagcgag gcggaagagc gaaacgaccg cccacgctat
      121 ctggatctca acgaaccgtc cacaccgcct aagccagcag tcgctgcgat gcccatcgcc
      181 gttccatcct tcctgggact tagcgatgtg ccgcaggtag aagccgagac cggtaacgag
      241 ctcggtgtcg aggaacctgc gcgcgggaga tggcggatct ggctggccgc agccgtgatc
      301 ctgattttcg gctttctagg agcgctggag tggcgggcac aagtgcgcca gacgaacaat
      361 ggccccgtcg aagttatcaa gatgaagatg tggaacctaa ctcacaacaa gccggcgaag
      421 agcgccagcg ccgaatctgc gtcgcccgcc actccttctg cctccgcttc caagcccgag
      481 atgcaggtcg agccaccgtc caatccgccg aagcaaaatc ccgccgcgag caccgcgact
      541 tctgccgcag cgaaaaatgc agcaaacgtt ccgaatacaa ctgccgcaac ggcgccgctc
      601 gcgagctctg tacgcgccac gcctgcgccc actggaaatc agccagtagc cgcgcagcct
      661 aatgtgacac cgccgcaaaa tccggcgctg aatgcgacgc gaactcctgc tgggcagaac
      721 acggtcgcga cgaaaaccgc gaccacaatg ccccccatgg acaaatcgaa attgattggg
      781 tcacgacagc ccgggtcggc cgccgtgtct acggctgaca aggcgaagcc gtcgccgcga
      841 gctgcggacg atagcgagga agcggtcgct aagagaataa ttcctggcgc cgaggagatg
      901 gccaaggcca acagcgccag tgattcggca gccgaagccg catggctgtg gaaggcgatc
      961 gccaggggca acccctctgc gccggtgcgg ctcgccgaca tgtacgtcaa aggcgacggc
     1021 gtgccgcgca gttgcgaaca ggcagtggtg ctcttgaaga ctgcggccga aaaagaaaac
     1081 gccctggccc gcaaacggct ggcttcgatg tatggctccg gaacttgcgt gcaacgcaac
     1141 cgcgtggagg cctatcgctg gctgagttca gccttggtcg ccgatcccaa cagcgaatgg
     1201 gcacagcagc accgcaacct gctctggcag cagatgaccc cggaagaacg cacctcggcg
     1261 gagaagtata ggtaagccgc tgttcgctat tcgctattcg ctcttggctc ttcgcttctg
     1321 gcgggcagcc aaagtcttcg cgaacaacta taatgatctc ggggtgaggt atcgcgcttc
     1381 gcccggagtt ttgaaaccac ttttcgttcc cttgcatcaa ccgccaactg gaggaaagaa
     1441 cagaatggca cacgaagttc ccgcgctgcc ctacgactac tctgccctag agccttacat
     1501 tgacacgcaa accatgcacc tgcaccatga caagcatcat gttgcctacg taaccaacct
     1561 gaatgccgcg ctggagaagg ctccggagct gcaaaaattg agcgccgaag aactggtcaa
     1621 gagcctggcg aacgtgccag aagcgattcg taccgcggtg cgcaacaatg ctggtgggca
     1681 cgtgaaccac tccatgtttt ggaccatcat gggcccgaac gccgggggag agcccagcgg
     1741 cgctatcgcc gatgccatca agaacacctt cggcgatttc aatagcttca aggaaaagtt
     1801 caacgatgcc ggcctcaagc agttcggcag cggctggaca tggctggtgc gcgaccgcgg
     1861 cggcaaactg ctggttatgg cgacgcccaa tcaggatagc ccgctcagcc aggatttctc
     1921 tcccatcatg ggcaacgacg tctgggagca cgcctattac ctgaagtacc agaaccgccg
     1981 cgccgactac ctgaaggaat ggtgg
//
LOCUS       DASRKK010000013         7355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_391579, whole genome shotgun sequence.
ACCESSION   DASRKK010000013 DASRKK010000000
VERSION     DASRKK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391579"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFC15_00175"
     CDS             complement(<1..821)
                     /locus_tag="VFC15_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08610.1"
                     /translation="MKRRSQYLLLNIVFLVALIAPTATIGATRPANSDEATTERYFQS
                     VRNNPNLLLAFLREMPKGGDLHNHLTGSVYSESYIQWAAEEGECVYPRTLSFSGPPCN
                     APAVPVRNAFADTELYANIIDAISMRNWQYSGQSGHDHFFAVFGKYAQATNGTTDKAL
                     AETAARAASQHEIYQELMLTPTGKDLETLAGSIGWDDDFGRLQQKLLAGGLPQILATA
                     SQQLNAAEVSRDQLLHCGVPAADPGCQVVQRYIAQVSRGKSKEVVFAQILTGFLLA"
     gene            1044..1799
                     /locus_tag="VFC15_00180"
     CDS             1044..1799
                     /locus_tag="VFC15_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08611.1"
                     /translation="MSPITISVAVFACVFGGALLGIFIHSLLPNEHLDSDSKEAVRLG
                     MALVGTTVALVLGLLIASAKGFYDTQNSEVTQLSADVVLLDKVLTHFGPETKEIRALL
                     RSSVARMVDATWAREGSDKTHSAPSAANEETLFDKIQELSPHNENQRFLQSQALSMAI
                     QLGQIRSLMLAQKSSSVPMPLLAVVVFWLALLFISFGLFVRPNVTVVVSLFASALAVC
                     GAIFLILEMYQPYAGLIQVSDAPLRAALAQLGQ"
     gene            1898..4411
                     /locus_tag="VFC15_00185"
     CDS             1898..4411
                     /locus_tag="VFC15_00185"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM08612.1"
                     /translation="MKGRSKILNRAPLVVAMAALSAQLAGAQQTSCAPGSPCATTTID
                     GKQLPPPPPKFEGKIGRNTAQSTPYWPARVEPPKGAPNILLIMTDDSGFGVPSTFGGV
                     IPTPALDRIAAMGLRYTNFNSTALCSPTRAALITGRNHHSAGFGVVSEQSTGYPGYDS
                     IITRDKATIGRILKDNGYRTSWFGKDHNVPAFQASQDGPFDQWPIGMGFEYFYGFIGG
                     DTSQWEPNLFRNMSPIYPYVGHPGWNLTTAQADDAIQYMKRINSLAPDQPFFIYYVPG
                     GTHAPHHPTPEWIKKISDMHLFDKGWDNLREQIFANQKKLGVIPQDAKMTPWPHDLLK
                     HWDELTPDEKKMFLRQVDVFAAYVAYTDNEIGRVIQAVDDLGKLDNTLIIYINGDNGT
                     SSEGTLIGTPNEVAMFNQVPVPVEVQLKYFYDAWGSDQTYAHMAVPWAWAFDTPFSWT
                     KQIASHFGGVRQGMAIAWPARITDKGGIRNQFHHMIDIVPTILEATGIPAPEIVDGIP
                     QKPIEGVSMVYTFDKANANAPSTHHTQYFEMMGDHSIYHDGWIASTKVMRPPWVTAGP
                     VSQDPASFPYELYDLTKDWTQSDNIADKNPEKVKEMDALFWQEAEKYQVLPLDATVAT
                     RLISPRPSLSAGRTVFTYSGEITGTPNGDAPSILNSSYDFKAEVEIPQSGGEGMIVTQ
                     GGRFGGYGFYVLKGKPVFTWNLVDLKRVKWQGTEVLSPGKHTLEFDFKYNGLGMGTLA
                     FNNMSGIGQGGTGVLKIDGKAVATQTMEHTLPLILQWDENFDVGADTGTPVDDQDYQV
                     PFTFTGKLDKLTLTIDRPQLTPADIQKLQQAERNNHASE"
     gene            4646..5179
                     /gene="hpt"
                     /locus_tag="VFC15_00190"
     CDS             4646..5179
                     /gene="hpt"
                     /locus_tag="VFC15_00190"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZM08613.1"
                     /translation="MDKLRVLIGRERIAAKVAEMGQRISQDFAGEPVVLIGVLKGATI
                     FLADLARQISLDVSFDFIAVSSYGNAKQQSGEVKLVKDVDRSMEGRNIILVEDILDTG
                     LTLTYIQSMLRAHQPKSLKIAALLDKVSRRTEPIHGDYVGFEIPDEFVVGYGLDFAEH
                     YRNLPDICVLESGPESE"
     gene            5200..5937
                     /gene="deoC"
                     /locus_tag="VFC15_00195"
     CDS             5200..5937
                     /gene="deoC"
                     /locus_tag="VFC15_00195"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZM08614.1"
                     /translation="MNSSVVIGAPAILVSDWRSAASLIDHTLLKPEATRDQVIALCDE
                     AVRYGFHAVMVNPANVALAAARLRGSPVKVGTVVGFPLGANLTVIKLAEAEAALRNGA
                     HELDMVLNIGALKSGDRMLVQQEMRSLARLAHDRGAILKVILETCLLNQVEKILVCSL
                     ALEESVDFVKTSTGFADAGATAADVVLMRGVVGLRVGVKAAGGIRTAAQFVDMLDAGA
                     NRIGTSASVEIVRELGAPADDRSNGNN"
     gene            5968..7215
                     /locus_tag="VFC15_00200"
     CDS             5968..7215
                     /locus_tag="VFC15_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZM08615.1"
                     /translation="MARLTGVLGILAILLFAYLFSTDRKAIRNRTVFVGLILQFVFAI
                     LVLRVSFGVRVMTIAGDAVNRLLSYSFAGSQFVFGEFGAKLSRFGVVFAFQVLPIIIF
                     IAAFFAILYYYGVMQFIIRQLAKVMMRFMGASGVESLDVAASIFMGQTEAPLTIRPFL
                     PKVTRSELMTIMTSGMAHVSGAMLGAYILLGIEARHLLGAVIMTAPGTILIAKMLVPE
                     TEKPLTAGTAKIDSVEFGERASNVLDAAAKGTTDGLRLALNVAAMLISFLALIALLDG
                     VLGGTHNLLAAHGFPWFPSKLESIFGVIFAPVAWLIGVPWHDCLAIGNLLGTRMVLNE
                     LVAFTMLSAQKATLDPRSFTIATFALCGFANFASIGIQIGGLSALAPSRRQDLAKLGF
                     RAMIAGTLANLMSASIVGILLRG"
BASE COUNT         1460 a         2174 c         2249 g         1472 t
ORIGIN      
        1 gccagcagaa agccggtgag aatctgggcg aagaccacct ctttggattt gccccttgaa
       61 acctgggcga tgtagcgctg tacgacctgg catcctggat cggcggcggg aacgccgcag
      121 tgcaggagtt ggtcgcgcga cacctctgcc gcgttgagtt gttgactggc agtcgccaaa
      181 atctgtggca acccgcccgc cagcagcttt tgttgcagcc ggccaaaatc atcgtcccag
      241 ccgatggacc cggcgagtgt ctccagatct ttgccggtcg gcgtaagcat cagctcctga
      301 tagatctcgt gctgggaagc ggcgcgggcg gccgtctccg ccagagcttt gtccgtggtg
      361 ccgttcgtcg cttgggcgta cttgccgaac acagcgaaga agtggtcgtg tccggattgg
      421 cccgagtact gccagttgcg catcgagata gcgtcaatga tgttggcata gagctcggta
      481 tctgcgaagg cgttcctgac cggcaccgca ggcgcgttgc acggtggccc gctgaaggac
      541 agggtcctgg gatagacgca ctcaccttcc tccgccgccc actgaatgta cgattccgaa
      601 taaacagatc cggtgaggtg attgtgcagg tcgccgccct tgggcatttc gcgcagaaag
      661 gccagcagca ggttggggtt gttgcgaacg ctttggaagt agcgctcggt ggtggcctca
      721 tcggagttgg ccggacgagt ggctccgatg gtcgctgtcg gtgcgattag cgctacgaga
      781 aatacaatgt tgagcaacag atactgggaa cgacgcttca tgcgcactcc tcaaaaccgc
      841 gccagccgag agtttcagga aatggcacga taataacagg cgaggtggct cgagctgcca
      901 tcgtaaggat gccccatcca agctggtttt gcttggacgg ggattttgct ttcatgtcac
      961 ggtgtgcgat gcgaaaatga gtaaatccgg gcggcccggc gcgcagagta cgccgaatct
     1021 tgggagggtg cacaacaact atcatgagtc ccatcaccat cagcgtggcc gtgttcgcgt
     1081 gcgtgtttgg cggagcgctg ctcggcatat tcatccattc ccttctgcca aacgagcacc
     1141 tcgattccga ttccaaggaa gctgtccggc ttgggatggc attggtcggg acgaccgtag
     1201 cgttggtgct cggactgctc atcgcttcgg ccaagggctt ctacgatacc caaaactccg
     1261 aagtgacgca gctctccgca gatgtcgtgt tgctggataa ggtcctgacc catttcggac
     1321 cagaaacgaa agaaatacgt gcgctgctgc gcagctccgt ggctcgcatg gtggatgcca
     1381 cttgggcgcg agaaggttcg gacaagaccc attccgcccc ttccgcagca aatgaagaaa
     1441 ccctctttga caaaattcag gagctctctc cccacaacga aaatcaacga ttcctacagt
     1501 cgcaggcgtt aagcatggcg attcaactag gccagatccg atcgctgatg ctcgcacaga
     1561 agtcctcgtc tgttcctatg ccgttgctgg cagtggtggt cttctggctc gccttgcttt
     1621 tcataagctt tggactcttc gttcgtccca atgtgacggt ggtggttagc ctattcgcct
     1681 cggcgctggc tgtttgcggt gcgatcttct tgatcctcga aatgtaccag ccttacgcgg
     1741 gattgattca ggtgtctgac gcacccctgc gtgcggccct ggcgcaactc ggacagtagc
     1801 tttaccgggg cactgtccgg tccttcgaac gggcgagacg ggtgtaggat ggttccgttt
     1861 tcgagatcat aagggaaatt ccggataagg agcactcatg aaaggaagat cgaaaatact
     1921 taatagagcg ccattggtcg tagccatggc tgctctgtcc gcccaactcg cgggagcgca
     1981 gcagacaagc tgcgccccag gctcgccttg cgcgaccacc acgatcgatg ggaagcaact
     2041 tccaccgcca ccgccgaagt tcgaaggaaa gatcgggcgc aacaccgcgc agtcgacgcc
     2101 ttactggccg gcccgcgtgg agccgcccaa gggcgccccg aacatcctgc tgatcatgac
     2161 cgacgattcc ggcttcggcg tccccagcac cttcggcggc gtgattccga ctccggcgct
     2221 cgaccggatc gcagcgatgg gcctgcgcta caccaacttc aattcgacgg ccctttgttc
     2281 tccgacgcgc gctgccctga tcaccgggcg caaccatcac tcggctggct tcggcgtggt
     2341 atccgagcag tccaccggtt atcccggcta tgacagcatc attacccgcg acaaggcgac
     2401 catcggtcgg atcctcaagg acaacggcta tcgcacctcg tggttcggca aggaccacaa
     2461 cgtgcccgcc ttccaggcca gccaggacgg tcccttcgac cagtggccca tcggcatggg
     2521 gttcgagtac ttctacgggt ttattggcgg ggacaccagc caatgggagc cgaacctgtt
     2581 ccgcaatatg tcgccaatct atccctacgt cggccatccc ggatggaacc tgaccacggc
     2641 gcaggcggat gatgccattc agtacatgaa gcgcatcaac tcgctcgccc cggaccaacc
     2701 cttcttcatc tactacgtcc ccggcggtac gcatgcgccg catcacccga caccggagtg
     2761 gatcaagaag atcagcgaca tgcacctgtt cgacaagggc tgggacaatt tgcgggagca
     2821 gattttcgcc aaccagaaga agctgggcgt gatcccgcag gatgccaaga tgacgccgtg
     2881 gccgcatgac ctgctcaagc attgggacga gctcacgccc gacgaaaaga agatgttcct
     2941 gcgtcaggtg gacgtcttcg ccgcttacgt cgcctatacc gacaacgaga tcgggcgcgt
     3001 gatccaggcg gtcgacgacc tgggcaagct cgacaacacg ctgatcatct acatcaacgg
     3061 cgacaacggc accagttccg aggggacgct gataggcacg cctaacgagg tggcgatgtt
     3121 caatcaggtc ccggtgccgg tcgaggtcca gttgaagtac ttttacgacg cctggggcag
     3181 cgaccagact tatgcccaca tggccgtacc gtgggcatgg gcgttcgaca cgccattctc
     3241 ctggaccaaa cagatcgcct cgcattttgg cggcgtgcgg cagggcatgg caatcgcgtg
     3301 gccggcgcgc atcacggaca agggcggcat ccgtaaccag tttcatcaca tgattgacat
     3361 cgtgccgacg atcctcgagg ccaccggcat tccggccccc gaaatagtgg acggcatccc
     3421 gcagaagccg atcgagggcg tcagcatggt ttacaccttt gacaaggcca acgccaacgc
     3481 gccgtcaacc caccacaccc agtacttcga gatgatgggc gaccactcca tctatcacga
     3541 cggctggatc gccagcacca aagtcatgcg gccaccctgg gtgactgcgg ggccggtgag
     3601 ccaagacccc gctagtttcc cctacgaact ctacgatctc accaaggact ggacgcaatc
     3661 cgacaacatt gctgacaaga acccggagaa ggtgaaggag atggacgccc tcttctggca
     3721 ggaggcggag aagtaccagg tgttgccact ggacgcgacg gtagcgaccc gcctcatttc
     3781 gccgcggccc agcctcagcg ccggccgcac cgtgtttacc tactccggtg agatcacggg
     3841 cacaccgaac ggtgacgcac ccagcatcct caattcgtcg tacgacttca aggccgaggt
     3901 cgagattccg cagagcggcg gagaaggcat gatcgtcacc cagggcggcc gcttcggcgg
     3961 ctacggcttc tacgtgctga agggcaaacc agtgttcacg tggaacctgg tcgatcttaa
     4021 acgcgtgaag tggcaaggaa cggaagtgct gtcgcccggc aagcacacgc tggagttcga
     4081 cttcaagtac aacggcctcg gcatgggtac gctcgccttc aacaacatga gcggcatcgg
     4141 ccaaggcggt acgggcgtcc tgaagatcga cggcaaggcg gtggccacgc agacgatgga
     4201 gcacaccctg cctctgatcc tgcaatggga cgagaacttt gatgtcggcg ccgacactgg
     4261 cactccggtg gacgaccagg actatcaggt tccgttcacc ttcaccggca agctcgacaa
     4321 actgacgctt acgattgacc ggccgcagtt gacgccggcg gacattcaga agctgcagca
     4381 ggctgaacgc aacaatcacg ccagcgaata gctggtgcca aggcaaacgg ggcagctcta
     4441 tcgggctgcc ctttgtttca gaaacccgta aactccaccc acggtgcagc cggcgaaaat
     4501 atcgccaatc tggcttgtcg ccccctcgaa accaatggcg agatgacctc ccaacctggc
     4561 ccattactcg gcccatatct cagttgagtt tccgtcggca tttctctacc atccagagtt
     4621 ctcatcctct tcaggcggca tgccggtgga caagctcagg gtgctgatcg ggcgcgaacg
     4681 catcgcggcg aaggtggccg agatggggca gcgcatcagc caggattttg ccggcgagcc
     4741 ggtcgttctg atcggagtgc tgaagggcgc caccattttt cttgccgacc tggcgcgaca
     4801 gatctcgcta gacgtcagct tcgacttcat cgcggtgtcg agctacggga acgccaagca
     4861 gcagagtggc gaggtcaagc tggtgaaaga tgtggaccgc tcgatggagg gcaggaacat
     4921 catcctggtg gaagatattc tggacaccgg actgacgttg acctacatcc aaagcatgtt
     4981 gcgcgcccac cagccgaaat cgctgaagat tgcggcgctg ttggacaagg tgtcgcggcg
     5041 taccgagccc atccacgggg actacgtggg tttcgagatt cctgacgagt ttgtggtggg
     5101 atacgggctt gactttgccg agcattatcg taatctgccg gatatttgtg tgctggagtc
     5161 cggccccgaa tcggaataga atctgtcagg ctcatgtccg tgaactcctc tgtcgtaatc
     5221 ggcgcaccgg ccattctcgt gagcgattgg cgcagcgcgg ccagcctcat cgatcacacc
     5281 ctgcttaagc ccgaggccac tcgcgaccag gtgatcgcgt tatgcgacga ggcggtccgg
     5341 tacggctttc atgcggtcat ggtgaatccg gccaacgtgg ccctggccgc tgctcgactg
     5401 cgcggctcgc cggtgaaagt tggcacggta gtcggctttc ctctgggcgc aaatcttacc
     5461 gtgatcaaac tggccgaggc ggaagccgct ttgcgcaacg gcgcgcatga gctggacatg
     5521 gtgctcaaca ttggggcgct gaagtcgggc gatcgaatgc tggtgcagca ggaaatgcgg
     5581 agcctggcgc ggctggcgca cgatcgcgga gccatcctga aggtgattct ggagacatgt
     5641 ctattgaacc aggtcgagaa gatcctggtt tgctcgctgg cgctggaaga gagcgtggat
     5701 tttgtgaaga cctccaccgg gttcgcggat gcgggcgcga cggccgctga cgtggtattg
     5761 atgcgaggcg tggttggcct cagagtcggc gttaaggcgg cgggcggcat tcgaactgcg
     5821 gcgcagttcg tggacatgct cgatgccggc gccaaccgca tcggcacgag cgccagcgtc
     5881 gagattgtgc gcgagttggg cgcaccagcg gacgatcgat ccaatggaaa caattaagct
     5941 tggttcgcga ctcacgcggg agttcgcatg gcgcgtctga ctggcgtgct gggcatcctc
     6001 gcgatcctgc tgtttgcgta cctcttctcg actgaccgga aagccatccg caatcgaact
     6061 gtattcgtag gactgatcct gcaatttgtg tttgccatcc tggtcttgcg cgtgagtttc
     6121 ggcgtgcgcg tcatgaccat cgcgggggac gcggtcaatc ggcttctcag ttattcattt
     6181 gccggatcgc aattcgtttt tggcgagttc ggggccaagc tctcccgctt cggcgtggtc
     6241 ttcgccttcc aggtgctgcc catcatcatt ttcatcgccg catttttcgc catcctttat
     6301 tactacggcg tgatgcagtt catcatccgg cagttggcga aagtcatgat gcgcttcatg
     6361 ggcgcaagtg gggttgagtc gctggacgtc gcggccagta tcttcatggg acaaacggaa
     6421 gctccgctca cgatccgtcc gttcctgccc aaggtcacgc gctccgaact gatgacgatc
     6481 atgaccagcg gcatggccca cgtttccgga gcgatgctgg gcgcctacat tctgctcggc
     6541 atcgaggcgc ggcatctgct gggggcggtg atcatgacgg cgccgggaac catcctgatc
     6601 gccaagatgc tggtgcccga gaccgaaaag cccttgaccg ccgggacggc aaagatcgat
     6661 tccgtagagt ttggcgagcg tgccagcaac gttctggacg cggccgcgaa aggcacgacc
     6721 gatggcctgc gccttgcgct gaacgttgcc gcgatgctga tttcgtttct ggcgctgatt
     6781 gcgctgctgg acggcgttct tggcggaact cacaatttac ttgcggcgca cggatttccc
     6841 tggttcccgt cgaagctgga gtcgatattc ggagtgatct tcgcacccgt ggcctggctg
     6901 atcggcgtgc catggcatga ctgtctggcc atcggaaacc tgctgggcac gcgcatggtg
     6961 ctgaacgagt tggtcgcctt caccatgttg agcgcgcaga aggccacgct cgatccgcgg
     7021 tcgttcacca tcgccacgtt tgcgctctgc ggattcgcga acttcgcctc catcggcatc
     7081 cagattgggg ggctcagcgc cctggcgccg agccgccgcc aagacctagc caagctcggc
     7141 tttcgcgcca tgatcgcagg cacgctcgcc aacctcatgt cggccagcat cgtgggcatt
     7201 ctgttgcggg ggtgaaaggc aggggctagg ggctagaggt caggggttag cgggtggcgg
     7261 gtggagaaaa gtgagaagcg gacagatgcg acgccgcgtc cgactgacgg ccagaaaggg
     7321 cggaatttga gttagccgcg ggcgtgagtc tctag
//
LOCUS       DASRKK010000014         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1271180, whole genome shotgun sequence.
ACCESSION   DASRKK010000014 DASRKK010000000
VERSION     DASRKK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271180"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1616
                     /locus_tag="VFC15_00205"
     CDS             447..1616
                     /locus_tag="VFC15_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067495976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZM08616.1"
                     /translation="MNAGKTLFAQLMDCLPWSTFTRIVARYRGDHSVQTFPCTEQYRA
                     MAFAQLTYRESLRDIEACLSAQPAKLWHMGFGRPVRRSTLADANEVRDWRIYEEFAQR
                     LIAQARRLYIGESLLEDLDNTVYALDSTTIDLCLSLFPWAHFRSTKAAVKMHTLLDLR
                     GNIPSFIHISDGKLHDVHALDMLVPEAGAIYVMDRGYVDFARLYGLHQAGAFFVIRAK
                     SNLSVHRVYSAATDRTTGVIADQTIALDGRYTSKDYPVHLRRVRFRDPETKKTLVFLT
                     NQTTLPALTICDLYKSRWQVELFFKWIKQNLRIKKFYGTSENAVKTQIWIAVSVYVLV
                     AIVRKRLKLDASLYTLMQLFSVTVFEKVPIESVISQTAYSSEHVMEDNQLNLFGD"
     gene            complement(1843..2889)
                     /locus_tag="VFC15_00210"
     CDS             complement(1843..2889)
                     /locus_tag="VFC15_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM08617.1"
                     /translation="MTITMIGLDTAKAVFQVHAVDKAGNVVIRRKIQRKDLITFFEKQ
                     EATTVVMEACGASHHWGRTLAVLGHDVKLIAPEAVKPFVKKGKKNDAADAAAICTAAH
                     QAEMKFVPVKTMEQQAILAVHTARALLVKQQTMLANAVRSLASEFGLTVPKGIGNVEG
                     LAEQVEEEKSLPEEARQVAKELKGRLSELVVHIGTLETKIVRHARNDARARRLATIPG
                     IGPITASLIAATVADFSVFKNARQFAAWLGLVPRQHSTAGKTRLGRITKAGNSEIRRL
                     LVLGATAMLKVAGQWNSVAGAWFRGVLERRPARLATVALANKTARIIWALMTHNEVYR
                     PSGRSAQPQARAAV"
BASE COUNT          566 a          897 c          831 g          657 t
ORIGIN      
        1 gcgtacgacc gcatatagct ggcgcccttc cgggttccat cgctcggaga atagtactgg
       61 tcagccagca tattaaggtc tggattgata atatttatca tgcgtcctct ctatcacccg
      121 ccgcaaattt cgcaagatca gtggctccag gcacattgcg gcgtgacccc gacacggtgc
      181 gctgacaaca aacgatgcct cccgggtcgg cccttgccgg atcagagcga agggcctttg
      241 agcgcgctcc cctgagccta cgatcaggat agcgtctgtc gcgcggtcaa tgaccgcgat
      301 cgcgatgttg cgtcactgct gtccggagta atgcgaacga actgctccga agcctttgac
      361 ctgtaacggt tttacgacga ttttcggtgg tgtcgatttg aagttggcgg cggtttcccc
      421 gacccttttc cggggagggc ctggccatga acgccgggaa gacgctgttc gcgcaactga
      481 tggattgcct gccttggagt acgttcacgc ggatcgtcgc gcggtatcgt ggtgaccact
      541 cggtgcaaac atttccgtgc accgaacaat accgggcgat ggcattcgcc caactgacat
      601 accgcgaaag cttgcgcgat attgaggcgt gcctgtcggc acaacccgcg aagctttggc
      661 atatgggatt tggcagaccg gtccggcgct cgacgctggc ggacgccaac gaagtccgtg
      721 actggcgcat ctacgaggag ttcgcccagc ggctgatcgc acaggcgaga cggctctata
      781 tcggtgagag cctgttggag gacctggaca acaccgttta cgcgttggac tcgaccacta
      841 tcgatttgtg cctgtcgctg ttcccatggg cgcatttccg gtcaacaaaa gccgcggtga
      901 agatgcacac attgctggat ttgcgcggca acatcccgag tttcatccac atttccgacg
      961 gaaagctgca cgatgtgcat gcgctcgaca tgctcgtgcc ggaggctggc gccatctacg
     1021 tcatggaccg tggctacgtc gatttcgctc gtttgtacgg gctgcaccaa gccggtgcct
     1081 ttttcgtgat ccgcgccaaa tccaacctca gtgttcatcg cgtgtattcg gccgccaccg
     1141 atcgcacgac cggcgtcatc gccgatcaga cgattgcact ggacgggcgc tacaccagca
     1201 aagactaccc ggttcatctc cggcgcgtgc gcttcagaga ccccgaaacc aaaaagacgt
     1261 tggtgttcct gaccaatcag acaaccttgc cggcattgac gatctgcgac ctatacaaaa
     1321 gtcgctggca ggtagaactg ttcttcaagt ggattaaaca gaacctccgg atcaagaagt
     1381 tttatggcac ctccgagaac gcggtgaaga cccagatatg gatcgcggta tcggtctacg
     1441 tgttggtggc catcgttcgc aagcggctga agctggacgc ctcactctac acattgatgc
     1501 agctattttc ggtgacagta tttgaaaagg tcccgataga atcagtgatt tcccaaacag
     1561 cttacagttc cgaacacgtc atggaggata accaattgaa tttgttcggt gattaaccgg
     1621 acagcagtga tgttcgacct ttttgtgcga ctgtgtcgct ggccatcatg ggggttcgct
     1681 acgtgtcggg caccaatcaa cctggcgtgc tcaaggcaca cggacataaa cggtttggcc
     1741 cggactgtct ttacccagtc tcccatcacg cctttgcagc aacctcctgc cggattagtc
     1801 aggcgggctt gcgccgtcta tccaccgttc catacgggaa tgtcacaccg cggctctcgc
     1861 ttgcggctgt gccgaacggc ccgacggacg gtagacctcg ttgtgcgtca tcaacgccca
     1921 gatgatgcgc gccgtcttgt tcgccagcgc gacggtcgcc agtctggctg ggcggcgctc
     1981 cagaacgccc ctgaaccatg cacccgcaac gctgttccat tgccccgcga ctttgagcat
     2041 ggccgtggct cccagcacca acaaccgcct tatctccgag ttgcccgcct tggtgatacg
     2101 ccccagacgc gttttcccgg cggtcgaatg ctgccgcggc accagaccga gccacgcggc
     2161 gaattgccgc gcgttcttga acacgctgaa atcggcgacc gtcgcagcga tcagcgacgc
     2221 ggtgatcggc ccgatgccgg ggatggtggc caaacgacgt gctcgggcat cgttccgcgc
     2281 gtggcgcacg attttcgtct cgagagttcc gatgtggaca accaattcgc tgagccgacc
     2341 tttcaattcc ttggccactt gccgcgcttc ttcgggcaag gatttctctt cctccacctg
     2401 ctctgcgagg ccctcgacgt tgcctatgcc tttcggaacg gtcagaccga actcggatgc
     2461 cagactgcgc actgcgttcg ccagcattgt ctgctgcttg accagcaacg ccctcgccgt
     2521 atgcacggca agaatagcct gctgctccat tgtcttgact gggacgaact tcatttccgc
     2581 ctggtgggct gcggtgcaga ttgccgcggc gtcggccgca tcgttcttct tgcccttctt
     2641 cacgaacggt ttgaccgcct ccggcgcgat taacttcacg tcatgcccga gaacggccag
     2701 cgtgcgcccc caatgatggg acgcaccgca cgcctccatc acgacggtgg ttgcttcctg
     2761 cttctcgaaa aatgtgatca ggtctttccg ctggattttg cggcggatca cgacgtttcc
     2821 ggccttatca acagcgtgaa cctggaacac cgctttggcg gtgtcgaggc caatcatggt
     2881 aatggtcatc gcgggcggtc cctttctggc tgttttgcga cgaccggatc atggcacatc
     2941 gatgcctggg g
//
LOCUS       DASRKK010000015         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_587046, whole genome shotgun sequence.
ACCESSION   DASRKK010000015 DASRKK010000000
VERSION     DASRKK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587046"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1551
                     /locus_tag="VFC15_00215"
     CDS             259..1551
                     /locus_tag="VFC15_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM08618.1"
                     /translation="MPYSPRKIRIACNHAGLTHYGGIYFFHEFLRVLQLRHFLATHLT
                     YPRRNSRYYLSQMILALMYPIVLGLDRLETASFLRSNGTFQYLTGLPSFPDPQTLRRF
                     LHHAPDAFWQQMHRVNDRLLQTFIHLPTQRSRLIFDLDSTVVTVFGSQEDAAVGYNPR
                     YRGKKSYDPLLCMEANSSYLWDAELRPGNAGTWDGSKEVLGTSFANVPPEIRELRVRA
                     DAGFGFNPVLVTLEERPAQYAVVARLTPAFKRLLSGVSYEPVNRKWEMAEFEHRPHGW
                     PKARRFVMARRFVQQEEAETTLFAMGRYIYRAWVTNLPLTPAGIWHFYDGRAGMEPRI
                     CELREDYALRKIPTASFAANALYLEIVRLAYNLVTAFQRNCLDESWQNLTLQKLRYKL
                     FLIPGELTRPQNRPVPRLRPSPMIESYSGPQNSDQAIS"
     gene            1587..2685
                     /locus_tag="VFC15_00220"
                     /pseudo
     CDS             1587..2685
                     /locus_tag="VFC15_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612102.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          642 a          852 c          892 g          598 t
ORIGIN      
        1 ggatatccgg gtccagtaga acaagatttg tgcctgcatg atagcgtttc gcgtacttgc
       61 cccgtacccc tgccttgagc aactcccgaa gatcgtactc cgggcgtaac tcgtcgttcg
      121 tcctatcctt gagcttcctc atacttaaat ccggcgcatt ctggcctccc cagaggccgc
      181 caaacgccgt gttgacaggc tctgggtgca tgttttagaa tttcaccaat tcctaaccat
      241 cggttaggga ttcgtgaaat gccatattcg ccgcgaaaga ttagaatcgc ctgtaatcat
      301 gcgggtctga cgcattacgg cggcatctac ttctttcatg aatttctgcg agtgctgcag
      361 ctccgccatt tcctggccac gcacctgacg taccccagac gcaacagccg gtactacttg
      421 tcccaaatga tattggccct gatgtaccct atcgtcctgg gcttggatcg cttggaaacc
      481 gcgtcattct tacgttccaa cggcaccttc caatacctca caggcttgcc gagcttcccc
      541 gatcctcaga ctttgcgccg cttcttacac cacgcacctg acgccttctg gcaacagatg
      601 caccgcgtca acgaccgact gctgcaaact ttcatccact tgcccactca gcgctccaga
      661 ttgatcttcg atttggacag caccgtggtt acggtattcg ggagtcagga agatgccgcc
      721 gtcggttaca atccccgcta tcgtggcaag aaatcctacg acccactgct ctgtatggag
      781 gccaattcat cctacctctg ggatgccgaa ctccgccccg gaaacgccgg cacctgggat
      841 ggcagcaagg aagtgctggg caccagcttc gccaatgttc cacccgagat tcgcgagttg
      901 cgagtccgcg ccgacgccgg cttcggcttc aatcccgtgc tcgtcacctt ggaagagcgg
      961 cctgcacaat atgcggtggt cgctcgtttg accccagcgt tcaagcgact cctgtcaggc
     1021 gtctcgtatg aaccagttaa tcgcaagtgg gagatggccg agttcgagca ccgtccccat
     1081 ggttggccaa aggcccgtcg tttcgtgatg gcgcgaagat tcgttcagca agaggaagcc
     1141 gagaccaccc tgtttgccat gggccgctac atctatcgtg cctgggtcac caacctgccg
     1201 ctgactccag ccgggatctg gcatttctac gacggacgtg ccggaatgga accacgaatt
     1261 tgcgaactgc gcgaagacta tgcactgcgc aagattccaa ccgcgtcgtt cgccgccaac
     1321 gcgctctacc tggaaatcgt ccgcctcgcg tacaatctgg tgactgcatt ccagcgaaac
     1381 tgtctggatg aatcctggca aaacctcaca ctacagaagc tccgctataa gctgtttctc
     1441 attcccggcg aactgacccg cccacaaaac cggccggtgc ctaggctcag accttccccg
     1501 atgatagaaa gctatagtgg tccccagaat tcagaccagg caataagcta agatttcggc
     1561 ggtagtcgtt gacagaggag aggtcgatga cgacgacgag aaaacagtac agtccgaagt
     1621 tcaaggcgcg ggtggcggtc gaggcgatcc ggggcgagaa gacgctcagc cagttgggat
     1681 cgcagttcaa ggtgcatccc atccagattg cgaagtggag gaagtccgca ctggagcaac
     1741 tgccggagct gttcgtggac gggcggacgc gcaaggggcg gaacggggaa gccgacagcg
     1801 acgcgctcta cgaagagatt gggcgtctga aggtggaact ggactggctc aaaaaaaaag
     1861 tcggcatgct cgattgagga gatgcggccg ctggtggagc ggggccgccc ggagatcagt
     1921 gtgcggcggc aatgcgagtt gctgggcgtc aatcgctcgg ggttgtatta cgagccggtg
     1981 ggcgagagcg aagagaacct tctgctcatg cggttgctgg acgagcagta tacacggacg
     2041 ccgttctatg ggagccggaa aatgacggag tggttggcga cccgaggcca tgaagtgaac
     2101 cgcaagcgcg tctcgcggct gatggagtca ctgggcctgg aagccgtata tccgaagccg
     2161 aagctgagcc agcccgggga caaccaccgg atctacccgt acttgttgag aggaaccacg
     2221 gtggagcgcg tcaaccaggt gtggagcacg gacatcacgt atatccggat ggcgcagggt
     2281 ttcttgtatc tggtcgcggt aatggactgg ttcagccggt tcgtactgag ctggtcgctg
     2341 tcgctgacga tggaggtgga gttctgtgtg gaggctctga agcgggcgct gcggcgggga
     2401 cggccggaca tcttcaacag cgaccagggg tcgcagttca ccagcgagaa gttcaccggg
     2461 gagttggcgg agcgcgagat cgcgatcagc atggacgggc ggggacgctg catggacaac
     2521 atcttcgtcg agcggttgtg gcgctcgctg aaatacgagg aggtgtactt gaaggattac
     2581 gcgtcggtga cggaggcgcg agcgggcatc gccggctact tccagttcta caaccacgag
     2641 cgcttgcacc agagcctcga ctaccggacg ccggcggcga tctacctggc gtgaggacaa
     2701 ctgcgatgac cggacgaact ctaaggaagg gagcgttgct ggactcgggg actccgtccc
     2761 cgaacccctg ggatttatcg ctttcgcgcc agaatgtctg ttctacaatg aaggcacttg
     2821 aacggaggat agggctccgc agggatgcga cccgagcgcc gattcaaggg ccggaatggc
     2881 aaggggcggg cttccatagc cgcccccaaa actcaaaccc aagaccccgc cgggcattag
     2941 cttattgcgg gcaaaaaatg gtcttgacaa tggggtccac ttta
//
LOCUS       DASRKK010000016         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_684844, whole genome shotgun sequence.
ACCESSION   DASRKK010000016 DASRKK010000000
VERSION     DASRKK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684844"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFC15_00225"
     CDS             <1..855
                     /locus_tag="VFC15_00225"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZM08619.1"
                     /translation="RGPKNRAEELRVELYKKINELGIGAEGLGGLITVLDVKVKDYPT
                     HAANLPVAMIPNCAATRHAHFVLDGSGAVTLQPPSLKDWPELTYNPAGARRVNLDTVT
                     RDEVATWKTGEVILLSGKLLTGRDAAHKRMTEMLKRGEQLPVDFTNRFIYYVGPVDPV
                     RNEVVGPAGPTTASRMDKFTRQMLKETGLLGMVGKSERGPEGIAAIRDHKAVYLMAVG
                     GAAYLVSKAIKKSRVLGFADLGMEAIYEFEVEDMPVTVAVDVHGNSVHESGPKEWQAK
                     IAELVEIG"
     gene            977..1858
                     /locus_tag="VFC15_00230"
     CDS             977..1858
                     /locus_tag="VFC15_00230"
                     /inference="COORDINATES: protein motif:HMM:NF018400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HZM08620.1"
                     /translation="MELQEILDSLDRDTGRYPRAAVTEAIARRDEIVPALLATLEDVA
                     RDPVPYTSKMRWIHVFAMYLLAQFREVRAYPLLVRIFSVPGEFAFDLVGDTVTEDLGQ
                     ILASVSGGDIGGMAALVENEAANEYVRAAGLTGLLTLCACGTLSREQVMTYFKSLFQK
                     LRRTPDGVWSWLADSCADLCPAEVMEELRKAYEENLVDPSVIGWDDVEEALALGQEAA
                     MIRLCSKRHLISDVHKELSSWYCFNKDQPAPVSDRPLPSPKFDVIDPIYRTEPKVGRN
                     DPCPCGSGKKYKKCCSR"
     gene            1908..>2372
                     /locus_tag="VFC15_00235"
     CDS             1908..>2372
                     /locus_tag="VFC15_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZM08621.1"
                     /translation="MPAKSALRTEKDSLGTKEIPGHVYYGIQTARAIENFPISGLRTH
                     PSLIRAIAMVKEAAALANRELGLVDEKTANAIIEAAKEVQQGKWDQEFVVDVFQAGAG
                     VSFHMNSNEVIANRASELLGGNLGEYKHVHPNDHVNYGQSTNDVFPTAMRLAA"
BASE COUNT          516 a          680 c          721 g          455 t
ORIGIN      
        1 cgcgggccga agaaccgcgc cgaggagttg cgggtcgagt tatacaaaaa gatcaacgag
       61 cttggcatcg gcgcggaagg cctgggcgga ctgatcacgg tactcgacgt aaaggtgaag
      121 gactatccca cgcacgccgc caacctgccc gtagcgatga ttccgaattg cgcggccaca
      181 cgtcatgctc acttcgttct cgacggctcc ggtgcggtca cgttgcagcc gccgtcgctg
      241 aaggactggc cggagttaac ctacaatccg gcgggcgcgc gacgcgtgaa cctcgacacg
      301 gtcacgcgcg acgaagtggc gacgtggaag actggcgagg tgattctcct tagcggcaag
      361 ttgctcaccg gtcgcgacgc cgctcacaag cgcatgacgg agatgctcaa gcgcggcgaa
      421 cagcttccgg tggatttcac taatcgcttc atctactacg tcggaccggt tgatccggtg
      481 aggaatgaag tggttggccc agcaggtccg acaaccgcct cacgtatgga caagttcacg
      541 cgccagatgc tgaaggagac tggcctgctc ggtatggtgg gcaaatcgga acgtggaccg
      601 gaaggcatcg cggccattcg cgaccacaaa gcggtttacc tgatggcggt tggcggcgcc
      661 gcttacctgg tctcgaaggc aatcaagaag tcacgcgtgc tgggcttcgc cgatctgggc
      721 atggaagcca tctacgaatt cgaggtcgag gacatgccgg tcacggtcgc cgtggatgtg
      781 cacggtaatt ccgtgcacga gtccggtccg aaagagtggc aggccaagat cgcggagttg
      841 gtggagattg ggtaacgctc tgtcttcgac tgtaagagcc gctggccacc accccagcgg
      901 cttttgattt cggaccacat gagcgggccg ggggacgtaa acaacggcta cgagcgcgtg
      961 ctaacctgac accgcgatgg aactgcaaga aatcctggat tccctagatc gtgacaccgg
     1021 ccgctatcct agagcggctg ttacggaggc cattgcccgc cgcgacgaaa tcgtccctgc
     1081 acttctggcg actctcgaag acgtcgcgcg tgaccccgta ccgtatacca gcaagatgcg
     1141 ttggattcac gtttttgcca tgtacctcct ggcacagttt cgcgaggttc gcgcctaccc
     1201 gctgctggtg aggatcttct ccgtgcccgg cgaatttgca tttgacctcg ttggcgacac
     1261 ggttacggag gacttgggac agattctagc ctctgtgtct ggcggtgata tcggcggcat
     1321 ggcggcactg gtggaaaacg aggccgcgaa cgaatacgtg agagcagcgg gtctaaccgg
     1381 gttgttgacc ctgtgcgctt gcggcacgct cagccgcgaa caagtcatga cctatttcaa
     1441 gagcctcttt caaaaactga gacgtacccc agatggcgta tggagttggt tggccgacag
     1501 ttgcgccgat ctctgtcccg cggaggtcat ggaggagctt cggaaggcgt atgaagaaaa
     1561 ccttgtcgat cccagtgtta tcggctggga tgacgtggaa gaggcgttgg cgctgggtca
     1621 ggaagcagcg atgatcaggc tttgcagtaa gcggcacctg atttcagatg tacacaaaga
     1681 gttgtcgtcg tggtattgct tcaacaaaga ccaaccggcg ccagtctccg acagacccct
     1741 tccatctccg aagtttgatg tcatcgatcc gatctataga actgagccaa aggtaggacg
     1801 caacgatccc tgtccgtgcg gcagcggcaa gaaatacaag aagtgttgca gcaggtagat
     1861 gatgctcatc aggtctcgcg agcatctctt ccgtagagga tgaattcatg ccagccaagt
     1921 cagcgcttcg caccgaaaaa gactctctcg gcaccaaaga aatccccggc cacgtctact
     1981 acggcatcca gacggcgcgt gccatcgaga actttcccat cagcggattg cgcacccatc
     2041 cctccctgat tcgtgctatc gcgatggtga aggaagctgc ggcgctcgcc aaccgcgaac
     2101 tcggcctggt ggacgagaag accgccaacg ccatcatcga ggccgccaag gaagttcaac
     2161 agggcaagtg ggaccaggaa ttcgtcgtcg acgttttcca ggcgggcgcg ggcgtcagct
     2221 tccacatgaa cagcaacgag gtcatcgcca atcgcgcttc cgagttgctg ggcggcaacc
     2281 tgggcgagta caagcacgtt catcccaacg accacgtcaa ctacgggcaa tcgacgaacg
     2341 acgtcttccc caccgcgatg cgtctcgccg cg
//
LOCUS       DASRKK010000017         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_196172, whole genome shotgun sequence.
ACCESSION   DASRKK010000017 DASRKK010000000
VERSION     DASRKK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196172"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFC15_00240"
     CDS             <1..304
                     /locus_tag="VFC15_00240"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZM08622.1"
                     /translation="DANSRKGKSLPPDDTHQEAAYLKMLGEKQCPVRIKLMDGDIVRG
                     WIEYYDRRMVRLTREGAPNLFIFKHEIAYIAEDTARKPRPDGPAPAKNGNSASGSE"
     gene            301..1134
                     /locus_tag="VFC15_00245"
     CDS             301..1134
                     /locus_tag="VFC15_00245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZM08623.1"
                     /translation="MTHYLPEVEAIAREAGALLMGYFAGHLTIEYKGDVDLVTEADRA
                     SEKLIVERLRARWPEHGIVGEEGTRSGPGADYRWYVDPLDGTTNFAHGYPVFCISIGL
                     VRKDEQIEAGVLYDPTRDEMFSAERGLGATLNGKPIYVSKTTKLAESILGTGFPSHKR
                     HKNPNIHFYHQITLRSHGVRRAGSAALDLANVASGRYDGFWEFNLNPWDTAAGALLVQ
                     EAGGRVTRFDGSPFRLDSREVLASNGPIHQELLTNFEEIFAGRGLEELPSPVEYAKSR
                     K"
     gene            1189..1782
                     /locus_tag="VFC15_00250"
     CDS             1189..1782
                     /locus_tag="VFC15_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08624.1"
                     /translation="MHRFTTRGLFLWFCLLSLCAFAQENRIVHVGVATMKNAAGLSVP
                     GNLERDRLVTALNQQKPDKKLHIKVQGVPLDGITPDDVGAEAKDKKCDYVVYTTLVEL
                     RSSTDPAMPRPGTIETNPNGVWNQRNDPRGQAMNPEYRATVEYELYRSGDNAAISGAP
                     FSTQEAMPQEAVVSQVMDRIAMRVFADVKKGPQTLPE"
     gene            1878..>2323
                     /locus_tag="VFC15_00255"
     CDS             1878..>2323
                     /locus_tag="VFC15_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08625.1"
                     /translation="MNNSLLNRTVSKRPDMVRLQGRILFLTEDPELIKRQLAGEDLPW
                     DTQQPERNPKLRDDISTDEITPAHICFFFDETLGEFPYTGLKCGDELPIKRGDVKRGG
                     FVCCVSGKRRGKGSSREQSPYAEMSAGIKLVIAENIERIYKQNCQNL"
BASE COUNT          486 a          704 c          702 g          431 t
ORIGIN      
        1 cgacgccaac tcgcgcaagg gcaagtctct accgcctgac gacactcacc aggaagcggc
       61 ttacctcaag atgctgggcg agaagcagtg tcccgttcgc atcaagttga tggacggcga
      121 cattgtccgc ggctggatcg agtattatga ccggcgcatg gtgcggctga cccgcgaggg
      181 agcgcccaac ctgttcatct tcaaacacga gattgcctac atcgcagagg acacggcacg
      241 caagccgcgt ccggatggcc ctgccccggc caagaacggc aactctgcaa gtggttcgga
      301 atgacgcact acctgccaga ggtggaagcg atcgcccgag aggctggggc gctcctcatg
      361 ggctacttcg ccggccacct caccatagag tacaaaggcg acgttgattt agtgaccgag
      421 gccgaccgcg cctcggaaaa gctgatcgtc gagcgcttgc gcgcgcgctg gccggaacac
      481 ggaattgtcg gcgaagaggg cactcgcagc ggtcccggcg ccgactatcg ctggtatgtc
      541 gatccgctcg acggcaccac caattttgcc cacggctatc ctgtgttctg catttcgatc
      601 gggctagtgc gcaaggatga gcagatcgag gctggcgtac tctatgaccc gacacgcgac
      661 gagatgttct cggcggaacg cggactgggc gccacgctga acggtaagcc aatctacgtt
      721 tccaaaacga cgaagctggc ggaatcgatc ctcggcaccg gctttcccag ccacaagcgg
      781 cacaagaatc ccaacatcca cttttatcac cagatcacgt tgcgctcgca tggcgtgcgg
      841 cgcgctgggt cggcggcgct cgacttggcc aacgtcgcaa gcgggcgcta tgacggcttc
      901 tgggaattca acctgaatcc atgggacacc gccgcgggcg cacttttggt tcaggaagcc
      961 ggcggacgtg tgacccgctt tgacggttcg ccatttcgcc tcgacagtcg agaggtcctg
     1021 gccagtaacg ggcccatcca ccaggagctg cttacgaact tcgaagaaat cttcgccggt
     1081 cgcggactcg aggagttgcc ctctccggtg gaatacgcaa aatcgcggaa atagccgcgt
     1141 cttgcggctc gttacgtgtt taataccccg atgaggaggt atctccacat gcatcgtttc
     1201 acgactcggg ggttgttcct ctggttttgc ttgctttcac tctgtgcatt tgcccaggag
     1261 aatcgcatcg tccatgtcgg ggtggctacc atgaagaacg ccgcgggcct ttctgttccg
     1321 ggcaacctgg agcgagatcg gctggtcact gcgttaaacc agcagaagcc tgataagaag
     1381 ctgcacatca aggttcaagg tgtgcccctc gacggaataa cgcccgacga tgtcggcgcc
     1441 gaagccaagg acaagaagtg cgattatgtg gtgtacacca ctctggtcga gttgcgcagc
     1501 tcgaccgatc ccgcgatgcc tcggcctggc accatcgaaa ccaatcccaa cggcgtttgg
     1561 aaccagcgta atgatccccg cggacaggcc atgaacccag agtatcgcgc cacggtggaa
     1621 tacgagctgt atcgcagcgg agataatgcg gcgatttcgg gcgcgccttt ctcaacccag
     1681 gaggcgatgc cgcaggaggc agtggtttcg caggttatgg accgcatcgc catgcgcgtg
     1741 ttcgcggacg tgaaaaaggg accccagacg ttgccggagt agggtagatg cgggtgattc
     1801 ggcggactgg ccggtgaccc gaccggaccg ccttccggcc ctgcgtgcga tcacaatttc
     1861 tgcggtaaaa ttacgcggtg aacaattcgc tgttgaaccg caccgtttcc aagcgccccg
     1921 acatggttcg tctccagggg cgcattctgt tcctcaccga agaccccgaa ctcatcaagc
     1981 gccagcttgc gggcgaggac ctgccctggg acactcagca accggagcgc aatcccaagc
     2041 tgcgcgacga tatctccacc gacgaaatca cgccggcgca catctgcttc ttcttcgacg
     2101 agacgctggg cgaatttccc tacaccggcc tgaagtgcgg cgacgagctg cccatcaagc
     2161 gcggcgacgt gaagcgcggc ggcttcgtgt gttgtgtcag cggcaagcgg cgcggcaagg
     2221 gatcgagccg cgagcagtcg ccttacgccg agatgtcggc gggcatcaag ctggtgatcg
     2281 cagaaaacat cgagcgcatt tacaagcaga attgccagaa cct
//
LOCUS       DASRKK010000018         5538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1271263, whole genome shotgun sequence.
ACCESSION   DASRKK010000018 DASRKK010000000
VERSION     DASRKK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271263"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VFC15_00260"
     CDS             complement(<1..1458)
                     /locus_tag="VFC15_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM08626.1"
                     /translation="MDLGASARKAALGALAISGFLLVSGGAQIAAQSADKPNIVMLMT
                     DDTGWNDFGAYSLGGANLGHPTPNIDKMAKEGAVFTSWYGQASCTAGRASFMTGRIPI
                     RTALSIVVAPGDENYLRPETPTIAEFFKKNGYSTYFSGKWHMGDKPVSYPTNHGFDEM
                     KEFAAYYAGVYSYPDTSKNFHPWFPSYNPDFEKMFDSVVNLSEWEGVAGKPATKVATI
                     TYDYMATMDERMSDYAVDYIKAHAKSGKPFFMDVNFIKMHNPTNAAPDFRGKSHLGDY
                     SDSLMELDANVGKVIDAIHQYAPNTIVILTADNGAWLDAYPDAGTTPFRGEKGSAFEG
                     GWRVPAIMTWPGHISAGVQYGEMMSHIDCWSTLAAMAGITPPPHGEMKDNNGKPIYFD
                     SIDNSAYILGKQKHSARTSWVYINGEYLNAVRVDMSGDLNNLDLNIAWKFVWTAKDSW
                     LGPSQSLGSVPALYNLTMDPFERYDMIFNGAEGEMN"
     gene            complement(1603..2664)
                     /locus_tag="VFC15_00265"
     CDS             complement(1603..2664)
                     /locus_tag="VFC15_00265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM08627.1"
                     /translation="MKTRLALLSSLFLILVLAGCTNNPTPTTPTAGQPAATAAAVDPL
                     PSWNDGPAKQAILDFVKTTTDKASPKFVAPEDRIATFDQDGTTWVEQPIYSQVLFAFD
                     RVAEMAPQHPEWKTRMPFKAIVTGDKEAMAKFTLKDIELIAMTTHTGMTAEAFGPIVK
                     DWMAKAKHPKFDKPYPQMVYQPMLEVMKYLRDNGYRTYIVTGGGQDFVRAYALPVYGI
                     PPEQIIGSALDTQYAYNKDGQGILMRDPKLLLNNNGVGKAEDIYLFIGKHPKAAFGNT
                     DGDRQMLEYTQASGGASLEMLVLHDDATREFAYGPAQRLPDTKVGAFPQSLYDEAKAK
                     GWTVISMKNDWRQIFSWEK"
     gene            complement(3172..4899)
                     /locus_tag="VFC15_00270"
     CDS             complement(3172..4899)
                     /locus_tag="VFC15_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM08628.1"
                     /translation="MDFGAGVRKVAIGALVISSFLLAGDVTSAHAQAATSAKPNILFI
                     MADDIGWMQPSIYHRGLMVGETPNIDRIGHDGAIFMDYLGMQSCTSGRNAFFTGMYPL
                     RTGMIPPQLPGSPSYLRQGTPTLAVFLRDLGYTTGEFGKNHLGDHTESLPTAHGFQEY
                     WGYLYHLDAMQQVSFQDINSSTAVQAIAPPCKNTPIPGLPEVPGAVDPRTTICLTPPR
                     PVIWCNSSDGTLKNQTCKDQGPLTLERSKTVDEEISAKVVDFLDRNDPKKTNKPFFVW
                     YNPARMHVTTMLSDKYMAMVGTTGGKDWGVNEAGMKQLDDNIGVVLAKLEQMGQLDNT
                     IVVFTTDNGAQQVTFPDGGVTPFKGQKGSAWEGGYRCPMVVRWPGHIKPETVKSQMFA
                     ALDWLPTLVDIAGGPKGDGLKQQIEAGKYPGIVKTTLDGFDQRGYLEGTSENSARDVF
                     FYYSGSTPSAVRYKNWKMYYSMTDSGAAGWVMPLTTYHMTLVQNIKRDPFEQAVTGTE
                     KTVNSIGGALAGPVTAYLYDWNMLPIGQQLWYGQLMSYKTFPPMQAPETWNLDMILKQ
                     VQSAGHASD"
BASE COUNT         1087 a         1467 c         1672 g         1312 t
ORIGIN      
        1 attcatttca ccttcggcgc cgttgaagat catgtcgtat ctctcgaacg gatccatggt
       61 gaggttgtac agggcgggaa cagagcccag gctctgactc ggcccgagcc aggagtcctt
      121 tgcggtccag acgaacttcc acgcgatgtt cagatcgaga ttattgaggt ctcctgacat
      181 atccacgcgg acagcattga gatactcgcc attgatgtaa acccatgagg tgcgtgcgga
      241 atgcttctgc ttgcccagga tgtaggcgct gttgtcgatg ctgtcgaagt agatgggctt
      301 gccgttgttg tctttcattt cgccatgcgg cggaggagta atgcccgcca tcgcggccag
      361 ggttgaccag caatcgatgt gcgacatcat ttcgccgtac tgtactcccg ccgaaatgtg
      421 tcccggccat gtcatgatgg ccggcacgcg ccagccgcct tcgaacgccg agcccttctc
      481 gccgcggaac ggagtggtgc ccgcatccgg ataggcatcg agccacgcgc cattgtcggc
      541 ggtcaggatg acgatggtgt tgggggcgta ttggtgaatg gcatcgatca ccttgccgac
      601 gttagcatcg agttccatga gggagtcgga atagtcgccc aagtgcgact tcccgcggaa
      661 gtccggggcc gcattggttg ggttgtgcat cttaatgaaa ttcacgtcca tgaagaacgg
      721 tttgccgctc ttggcatgcg ccttaatgta gtcaaccgcg tagtcggaca tcctttcgtc
      781 catggtcgcc atgtagtcgt aggtaatggt ggcaaccttg gtcgcgggtt taccggctac
      841 gccttcccac tcgctcaggt tcaccaccga gtcgaacatt ttttcaaaat ccgggttata
      901 cgacggaaac cagggatgga aattcttgga agtatccggg taagaataca cgccggcgta
      961 gtaggcggcg aattctttca tttcgtcgaa gccatgattc gtaggatagg aaacaggctt
     1021 gtcgcccatg tgccacttgc cggagaagta ggttgagtag ccgttcttct tgaaaaactc
     1081 agcaatcgtt ggagtctctg ggcggagata gttctcgtcg ccaggggcaa ccacgatcga
     1141 gagtgcggtg cgaatgggaa tacgaccggt catgaacgag gcgcggcccg ccgtgcagct
     1201 cgcctgtccg taccaactgg tgaagacggc tccctctttg gccatcttgt cgatgtttgg
     1261 cgtcggatgg ccgaggttag cgcctccgag ggaataggca ccgaaatcgt tccatccggt
     1321 gtcgtcggtc atgagcatga cgatgttggg tttgtctgcg gattgcgccg cgatctgggc
     1381 cccaccactc accagcagga agccggaaat ggccagtgcg cccagcgctg cttttctagc
     1441 acttgctcct aaatccatat attgctcctt actattacgc actactggtt ggtccccatg
     1501 gacagttatg attcgattgc caagttacga tcagtccagt gatctcgaaa ctttcaaatg
     1561 tgttgatggc tacaaccata ctctggctgc ttcgttgttg gctcactttt cccagctaaa
     1621 aatctgcctc cagtcattct tcatgctgat cacagtccaa cccttagctt tggcttcgtc
     1681 gtatagagac tgcgggaagg cgccaacctt ggtgtcgggc aatctctgcg ccggaccata
     1741 ggcaaattcg cgtgtggcgt catcgtggag caccagcatt tccagcgagg ctccgccgct
     1801 ggcctgggtg tattccagca tctgccggtc gccatcggtg ttgccgaacg cggccttggg
     1861 atgcttaccg atgaagagat aaatatcttc ggccttgccc acgccattgt tgttgagcag
     1921 aagtttcggg tcgcgcatca ggatgccctg tccatccttg ttgtaggcgt actgggtgtc
     1981 gagggccgag ccgatgatct gttccggcgg gatgccatac accggcagcg cgtatgcgcg
     2041 gacgaagtcc tgtccgccgc cggtcacgat ataggtccgg tagccgttgt cgcggagata
     2101 cttcatgacc tccagcatgg gctggtacac catctgggga taaggcttgt cgaatttggg
     2161 atgcttggct ttcgccatcc agtccttcac gatcggcccg aaggcttccg cggtcatgcc
     2221 ggtgtgggta gtcatggcga tgagttcgat gtccttgagg gtgaacttcg ccatggcctc
     2281 tttgtcgccg gtgacgatgg ctttgaacgg catcctggtc ttccattccg gatgctgcgg
     2341 ggccatctcg gctacgcgat cgaaggcaaa caatacctgc gaatagatag gttgctcgac
     2401 ccaggtcgtg ccgtcctggt caaaggtagc gattcggtct tcgggcgcca cgaatttagg
     2461 actggctttg tcggtggtgg ttttgacgaa gtcgaggatg gcctgcttgg cggggccatc
     2521 attccaggaa ggcaaaggat caacagccgc agcggtcgcg gctggttggc ctgcggtggg
     2581 agtcgtgggt gtggggttat tcgtgcagcc agcaaggacg agaattagaa aaagagatga
     2641 gagcaatgcc agacgggttt tcattcttgg gtctcctaaa cttccgttac gattcgggcc
     2701 gcgagccgat cagaggacgc gagtgccgga ctatctcctg aagatgggac gcgccatttg
     2761 ccacattttc ttttgcacgt gttgtttgtt ttcatcgact taggctgaaa ttgttcgcat
     2821 gtcattaatt ctattagtag ttatagctat ccatgtcagc cagtaggact tagatggtca
     2881 atttttgcct ccagcagggc tggttgggcc gtcgtccgta gtccattcgc taccgactgc
     2941 gcatggtact ccagatctca gtgtaaagag tgtggggttg agagtcagtg ataaaagggg
     3001 cgatgaagtt acgaaggtgg ggggtcaccc aggttaggcc aaagaagctg gcgcgaagtt
     3061 ggggtaccga gttaatcatg gtaagaaggg cgaagaagga tgttctccct cttcgcccca
     3121 tttctaacaa cgcggattat cggaatccga gttcataact actaccgacg cctagtcgct
     3181 ggcgtggccc gcagactgca cctgcttcag gatcatgtcc aggttccagg tttccggcgc
     3241 ctgcatgggc gggaacgtct tgtacgacat gagctgcccg taccacagtt gctggccgat
     3301 cggcagcatg ttccagtcgt agaggtaggc ggtcaccggt ccagccaggg caccgccaat
     3361 gctgttgacg gtcttctcgg tccctgtgac agcctgctcg aaggggtcgc gcttgatgtt
     3421 ctgcaccagg gtcatgtgat aggtggtaag cggcatgacc cagccggctg ctcctgagtc
     3481 cgtcatggag taatacatct tccagttctt gtagcggacg gcggagggag tcgagccgga
     3541 gtagtagaag aagacatcac gcgccgagtt ttccgacgtg ccctcgagat acccgcgctg
     3601 gtcaaaaccg tcgagcgtgg ttttcacgat gccgggatac tttccagcct cgatctgctg
     3661 cttcaaaccg tcgcccttgg gaccgcccgc gatgtcaacc aatgtaggca gccagtcaag
     3721 cgccgcgaac atctggctct tcaccgtttc cggcttgatg tgacccggcc agcgaaccac
     3781 catcgggcag cggtagccgc cttcccaagc cgaacccttc tgacctttga acggtgtgac
     3841 gccgccatca gggaaagtaa cttgctgagc gccgttatcg gtggtgaaga cgacgatggt
     3901 gttgtcgagt tgacccatct gttccagctt ggccagcacc actccgatgt tgtcgtccaa
     3961 ctgcttcatg ccggcttcgt tgacgcccca gtctttgccg cccgtcgtgc ccaccatggc
     4021 catgtacttg tccgacagca tagtcgtgac gtgcatgcgc gccgggttgt accagacgaa
     4081 aaagggcttg ttggtcttct tggggtcatt gcgatcgagg aagtcgacga ctttagccga
     4141 aatctcctcg tccaccgtct tggagcgctc cagtgtcagc ggcccttggt ccttacaagt
     4201 ctggttcttc agagttccgt cggaggagtt gcaccagatc acaggacgag gaggcgtgag
     4261 gcatatcgtg gttcttggat ccacggcgcc gggtacttcc ggcagcccgg ggatcggggt
     4321 gttcttgcac ggcggcgcga tggcctgcac cgcggtgctg ctgttgatgt cctggaaact
     4381 cacctgctgc atcgcgtcga ggtggtagag gtagccccaa tactcttgga agccgtgagc
     4441 cgtgggcaga gactcggtgt ggtcgccaag atggttcttg ccgaactcgc cggtagtata
     4501 gccaagatcg cgcaggaaca ctgccagcgt tggggtgcct tgccgcagat aagacgggct
     4561 gccgggcaat tgcggcggaa tcattccggt gcgcagcgga tacatgccgg taaagaaggc
     4621 attgcgtccc gaggtacagc tctgcatgcc gaggtagtcc atgaagatcg cgccgtcatg
     4681 gccaatgcgg tcgatgttgg gtgtttcgcc caccatgagg ccgcggtgat agatgctcgg
     4741 ctgcatccag ccgatatcgt cggccatgat gaacagaatg ttaggcttcg cgctcgtggc
     4801 tgcttgcgcg tgcgcgctgg tgacatcgcc ggccagcaaa aagctggaga taacgagcgc
     4861 gcctattgct acttttcgaa ctcctgcacc aaagtccatg atgaatctcc tgttgcatca
     4921 attgctggtt ggcctccacg agggagggtc gcgatgggtg gaggggagcc catcagagga
     4981 tgtcaccagt aacgcagtgg cctcgcccgg tcgccagctg aatttccgga atgagagaag
     5041 ggtactccaa aatcgaattc aagctgctca aatcatcgag tcacatgccc atacagtttc
     5101 ggacttcggc gacaacttgg gccgggggtg agcacaggtt gcgcaacata agccactgtt
     5161 cgtgtacaga aaagtatagg cgtgtcatag ctatgcgtcc aacgctctta cgcataaggc
     5221 tgtagcttgg actcgtggat tttgaacttc ggtggcggct agtcttttcc aattgaagaa
     5281 tgagcctgga ggaagcctga ggatatggct gaggaaagct cctttcttga ttttgtattg
     5341 aacgagtcta gcagtcgccg ggggggtgga aatgtggcaa tcgcgaagcg atttcccagg
     5401 tgggtgcgaa acccgcaggg ttttgcatgc accgtcattt ccacggtcct tccgtaggct
     5461 gccgcaactg ctcaaagagt ttgcctttgg cttgctgcat gcgctgggcg gcttgggtat
     5521 cgctgactgc ggcggcga
//
LOCUS       DASRKK010000019         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_489437, whole genome shotgun sequence.
ACCESSION   DASRKK010000019 DASRKK010000000
VERSION     DASRKK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489437"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFC15_00275"
     CDS             <1..724
                     /locus_tag="VFC15_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZM08629.1"
                     /translation="SVVAQVVYPALSKIQDDRERFRAAYLRTCRLIGLLTFPLMLGLA
                     VTAQPFVRVFLGARWMPVAGLLIVFAPLGAAQSIYTTVGLIYNTQGRPDLQFRWAMFA
                     SVMYVGSFAVGLRWGILGVAGCYAFVWLLLMVPSFMIPFRLVELSGKQFLRTLWPTIW
                     MSFVMAAITEAWLQGLRRLGIQNAPVELISTVILGIVVYLTLVLSYKPPVLSELAVTL
                     KGSSRPALRKIADCLSSAADGQ"
     gene            1169..2212
                     /locus_tag="VFC15_00280"
     CDS             1169..2212
                     /locus_tag="VFC15_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZM08630.1"
                     /translation="MARDLDTASPAVETPPKPATMTAANNRRPNSSPIKRLKRWVKSL
                     LFTGCEQLNTTSSWIQDRILHTSLEQKRAADNSAIPVGEMDRDLLAMHIRWAGHNVEK
                     RVRTRGNPDRGGNRAGELKAAVAEWHRRGYPRRNFVAWAEENLRDYEKWLECGEPQFH
                     PEHDLPLFGPDSPVIEVLKNRVSTRFWKPVPVEEEKIRQIIGLATHAPTACNRQAWKL
                     YVCRNPDLSRAANAPGVLNPMLRAVAPVTIYIAIDTRLYPEVWAPAQDAGIIGLQLSL
                     ATTAVGLTGCLMYGAEMFPQEAWRRHYGIPDYQIMELMFLFGYPAERTLTNKRADAED
                     VAVFLDSAFEASG"
     gene            2213..4018
                     /locus_tag="VFC15_00285"
     CDS             2213..4018
                     /locus_tag="VFC15_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08631.1"
                     /translation="MISFARSVVDAITSSFGEAYVVERGQSPHTPRLYSESHYALALI
                     LLYLLDGHDDRRLQLAADRLNKWDQLGEPETFFNSFAILLSQILTVRYEIAHPELNVT
                     LDRLCRAHKSTYREAWADCCGNNMYVQQVLADLLLYPLATGNAVGDAEVHPILLEFAR
                     FRSPEGLYFDGPRAGTSERLFPLTYCMKFLFLLGISYKLQGHENFRKAFVDGLRAALP
                     LMTSEGGFSYFGRTDNSSFATGLTIFVLRLGAEVDSSRQSTYSTLARRQEQFYKSTPR
                     TTGSYLQVNRLHEPKTPSEYEWSRDAYSYPTQYSIASAAYVLLSEQLCSRLAVEQDGE
                     YEQCASVTYSEDLGIAKLCAPGVEAFVRTLSNTANHDRRYIGPSVLRFEARRQLTVGA
                     VPVTCSDDGSYQIQQTAALRILKNVWHKYHKGTDDLDARYTGFVPVVMDGGDILLPLR
                     VVRQRIEGCCIETEYEFGRRSFRGWPVVLDQLLLYWSALGLPRPQRHSTSRGFIAEPN
                     IHFIRRQHLTEAANLLIEDTLLGNLRYKVLWFSTRYVPGAAVEVSGLRRTGDAIGWGS
                     DGEMLFDLYAQRCATDEISYSCRLRGFQGSPKARN"
BASE COUNT          845 a         1219 c         1236 g          949 t
ORIGIN      
        1 ttcggtagtc gctcaggtcg tctatccggc gttgtccaag attcaggacg atcgcgagcg
       61 cttccgtgcc gcctatctgc ggacctgtcg gttgatcggg ctactcacct tccccctgat
      121 gcttggcctc gcagtgaccg cacagccttt cgtccgcgtt tttcttggag cccgatggat
      181 gccggtggct ggactgttga tcgttttcgc tcccttggga gccgcacaat cgatctacac
      241 cacggttggc ctcatctaca acacccaggg gcggcccgat cttcagtttc gttgggcaat
      301 gtttgccagc gtaatgtatg taggctcatt cgcggtgggt ctgcgctggg gcatcctggg
      361 ggtcgccggt tgctatgcgt tcgtctggct attgctgatg gtgccgtctt tcatgatccc
      421 atttcgtctt gtcgaactct cgggcaaaca gttccttcga accctgtggc caaccatatg
      481 gatgagcttc gtgatggctg ccataaccga agcttggttg caaggattgc gccgactggg
      541 gatccagaac gctcccgtgg aattgatcag caccgtgata ctcgggatcg tggtctattt
      601 gacgcttgta ttgtcataca agccacccgt gctttcggaa ctggctgtca ctctgaaggg
      661 ctcgtcccgc ccggcgctac gaaagattgc ggattgtctc tccagcgctg ccgacggcca
      721 gtagaggttg catagagctg gtttcataaa taggttccgg atcaagtcag gtacttacgc
      781 gcgttaaagt gcacttggca tagccgtcgg tatgcaaccg agtgaccggc agtgggactt
      841 gatcgagcaa ttctttcctg atccaaaacg gcggcgcgat ggccgtggtg ggccatgggc
      901 gttcactcgc gactgcattg agcgcatcct ctgggtgctg aaggccggtg cccggtggcg
      961 ggatcttcct cccggcacgg tatgataagc taggtttttt gcgggtgtag aaggaaattc
     1021 gaacaagttg atgcctccgc agacgggcgg acggccgggc gctgaaggct actacgaagc
     1081 cgataccaga tcgcctaaga ctatactttt tcggttccga cccgagcttg tggcaatgcc
     1141 atccggcacg tccgaaggac ttacattcat ggcgagagat ttggatacgg cgagccccgc
     1201 agtcgaaacc ccgcccaaac ctgcgacgat gaccgcggcc aacaaccgtc gcccgaacag
     1261 tagtcccatc aagcgcctga aacggtgggt caagtctttg ctgttcactg gctgcgagca
     1321 gctcaacact acctcgtcgt ggatccagga ccgcattctg cacacgagtc tggaacagaa
     1381 gcgtgcggcg gacaattccg caattcccgt cggcgaaatg gatcgtgacc tgttggccat
     1441 gcacatccgc tgggccggac acaacgttga gaagcgcgtg cgaacgagag ggaacccgga
     1501 ccgaggggga aatcgcgccg gcgaactcaa ggctgcagtc gcggaatggc atcggcgtgg
     1561 atacccgcgc cggaattttg tcgcgtgggc ggaggaaaac ctcagggatt acgagaagtg
     1621 gttggaatgc ggcgaaccgc agtttcatcc tgagcatgac ctaccgctgt ttggcccgga
     1681 ttcaccggtc attgaggtat taaagaaccg tgtgagtact cgtttttgga agccggttcc
     1741 cgtggaggag gaaaaaatcc ggcagatcat cgggctggct actcacgcgc cgaccgcatg
     1801 caatcggcaa gcctggaagt tgtacgtatg ccgcaatccc gatctcagcc gggccgccaa
     1861 tgccccaggg gtgttgaacc ccatgttgcg cgcagtcgcg ccggtcacca tctatattgc
     1921 aatagatacg cgcttgtatc ctgaggtgtg ggcgcctgca caagacgccg gcatcatcgg
     1981 tttgcaactc agcctcgcta ccactgccgt tggtctgacc ggctgcctga tgtatggggc
     2041 cgaaatgttc ccgcaggagg catggcgccg ccattacgga atacccgact atcaaatcat
     2101 ggaactcatg ttccttttcg gctatcccgc cgagcgcacg ttgaccaaca agcgcgccga
     2161 tgcggaggac gtggcggtct tcctggattc cgcattcgag gcttccggct agatgatatc
     2221 gtttgcccgt tccgtcgtgg atgccatcac cagttctttc ggtgaagcat acgtcgtcga
     2281 gcgtggtcaa agtccccaca cgccacggct ctactcggag tcgcattacg cgctcgcgct
     2341 catcctgctg tacttgctcg acggccacga tgaccggcgc ctgcaactcg ccgccgaccg
     2401 gctgaacaag tgggatcaac tgggagaacc ggagacattc ttcaacagct tcgccattct
     2461 gttgtcgcag atcttgacgg tccgctacga gattgcccac ccggaactga acgtcacact
     2521 ggatcggctg tgccgggccc acaaatctac atacagggaa gcatgggctg actgttgcgg
     2581 caacaacatg tacgtccagc aggtcctagc cgaccttctg ctgtacccgc tcgcgacggg
     2641 gaacgcagtt ggcgatgctg aggtccatcc gatcctgctg gagtttgctc gcttccgatc
     2701 tcccgaaggg ctttatttcg atggcccgcg cgccgggact agcgagcgtc tgttcccgct
     2761 gacctattgc atgaaattcc tgttcttgtt gggcatttca tacaagctgc aggggcatga
     2821 gaattttcgg aaagcattcg ttgacgggct gagggcggca ctcccgctga tgacgtcgga
     2881 gggcggattc tcctatttcg gccgcaccga caactccagc tttgctaccg ggctcacaat
     2941 tttcgtgctg cgcctgggtg cggaagttga ctcttcgagg cagagtacct actcgacact
     3001 cgctcgacga caggagcaat tctacaaatc gactccgcga accacgggta gttaccttca
     3061 ggtcaatagg cttcacgagc cgaagacccc ctccgaatac gagtggtccc gggacgctta
     3121 ctcgtacccg acgcagtact cgatcgcttc tgccgcttac gttctgctaa gcgaacaact
     3181 ttgctcccgt ctcgctgtag aacaggatgg cgaatacgag caatgcgcca gcgttacgta
     3241 ttccgaagat ctcggaatcg ccaagctctg tgctccgggc gtggaggcgt ttgtccgcac
     3301 attgtccaat accgcgaacc acgaccgtcg ctatatcggg ccgagcgtgt tgcgcttcga
     3361 agcgcggcgc caactcactg tgggtgcggt tcccgtcacc tgttctgatg atggctcgta
     3421 ccaaatccag cagaccgccg cattgcgtat cctgaagaat gtctggcaca agtaccacaa
     3481 agggacggat gatcttgatg cacgctacac cggtttcgtg cccgttgtga tggacggagg
     3541 cgacatcctg ctcccgctcc gagtcgtccg tcagcggatc gaggggtgct gcattgagac
     3601 ggaatacgaa ttcggccgaa gatcatttcg cggctggccg gtggtgctcg atcagttact
     3661 gttgtactgg agcgcgctcg gcctgccgcg gccccaacgg cattcaacat cccgcgggtt
     3721 cattgccgag ccgaacattc atttcatacg ccggcagcat cttaccgagg cggctaattt
     3781 gctgatcgaa gatacactgc tgggcaactt gcggtacaag gtgctgtggt tctccacgcg
     3841 atatgttccc ggtgcagcag tggaagttag cggccttcgt cgcaccggag atgcgatcgg
     3901 atggggcagc gacggcgaga tgctgttcga cttgtacgcg cagcgctgcg caacagacga
     3961 aatctcctac agttgtcgtc tacgcggatt tcagggatcg cctaaggcgc ggaactagga
     4021 gtgacagagg cgcataaagc ttggaaagtc tccggcttac aaagcgcgaa ccggagaaac
     4081 ttcccggtgg aatctaaagc ccgttcacag tccacggcat gatgccaatg gagcttgttc
     4141 atcagcttcg cgggcgaatg atagtacctg cgaacctgaa tcttgagatg cgggtccccc
     4201 aagttgactt cattatgttt tctaatgcga gctgtgccct gtactcttg
//
LOCUS       DASRKK010000020         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1076002, whole genome shotgun sequence.
ACCESSION   DASRKK010000020 DASRKK010000000
VERSION     DASRKK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076002"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2440)
                     /locus_tag="VFC15_00290"
     CDS             complement(<1..2440)
                     /locus_tag="VFC15_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM08632.1"
                     /translation="MDIVDQRAFGLPVSSISLKNSELEFDVDKVQGSYVGKLNPEATK
                     ISGSWSQGGAFLDLDFRRATDAVKAQPKPAAPSDIDGAWVGTIEASGIELRVVFHILN
                     TADGLTATMDSPDQGVNGVLATAVTRNGSSLKIEMKQKGAAYEGKVSSDLSTIDGTYT
                     WRAGSLSLVVKRVKHVSELELRSRPQDPVKPYPYSEHEVSYSNDKVRGVTLAGMMTVP
                     PGKGPFPAVLLIPGSGPHDRDETLLGHKPFLVLADFLTRKGIVVLRVDDRGIGKSTGE
                     RTDATTADFATDAEAGVAYLKTRSEVNPHKIGLIGHSEGGLIAPMVAATDPDVAFIVM
                     MAGPGVPGDEILVEQRLLISEASGVSHEAAEKNVALERELLTLVKQEKDDAVLQKELR
                     DKLVGVGSEAQINVQIKAITSPWIRYLITYDPATALRKVKCPVLAINGERDLQVSPKQ
                     NLPAIRKALEEGGNQQFEVDELPNLNHLFQTAKTGAPSEYGEIEQTISPVAMERIASW
                     ILMDPKPVSAFHVLDPAYSALSDYDRAIQDDSEVIRQNPKSADSFNDRGAAYLSAGEY
                     GRAIQDYDEAIRLNPKSAGAFTGRGSAYLGKRDYDRAIQDYDEAIRLDPKSAGAFTGR
                     GSAYLDRGDYDRAIQDFNEAILLDSKSAETFNGRGAAYFGKEDYGPAIQDYTAAIHLN
                     PDWAMARALLNRGLAHLYAGQSTEAQQDLSQNLRLHQTDLYSAIWLYLSRAKGGTDGK
                     DELKTNTAGFDLSKWPGPVIQLYLGQSTSEDALRAAGSDSDQKCEYSFYVGEYRALRG
                     ERPEALALFRSARDG"
BASE COUNT          672 a          973 c          907 g          749 t
ORIGIN      
        1 atccatcacg ggcactgcga aacagagcaa gagcttctgg gcgctcgcct cgcaaagcgc
       61 gatactctcc cacgtagaag ctatattcac acttctggtc gctgtcactg ccggccgcac
      121 gcaaggcatc ttccgatgtg ctttgaccaa gataaagctg gatcacgggc cccggccatt
      181 ttgataaatc aaagcccgca gtattcgttt tcagctcatc ttttccatcc gttccaccct
      241 tggcgcgtga caggtagagc catatggcgc tgtacaagtc tgtctgatgc aagcgcaaat
      301 tctggctaag atcctgctgc gcctcagttg actgacctgc atagagatgt gccagtcctc
      361 gattcaggag cgcacgcgcc atcgcccaat ccggattcag gtgaatggcc gcagtgtaat
      421 cctgaatcgc ggggccgtag tcctctttgc cgaagtaagc cgcgccacgg ccattgaacg
      481 tttccgcaga ttttgaatca aggagtatgg cctcgttgaa gtcctgaatc gcccggtcgt
      541 agtctcctct gtcgaggtaa gccgagccgc ggccagtgaa cgcccccgct gattttggat
      601 ccaggcggat ggcctcgtca tagtcctgaa tggctcggtc gtagtctctc ttgccgaggt
      661 aagccgagcc gcggccagtg aacgcccccg ctgattttgg attcaggcga atggcctcgt
      721 catagtcctg aatggcgcga ccgtattcgc ccgccgagag gtaagccgcg ccacggtcat
      781 tgaatgaatc cgctgatttc ggattctggc gaatgacctc gctgtcgtct tgaatggcgc
      841 ggtcgtagtc gcttagggcg gagtaagccg ggtcgaggac atggaacgcg ctgacaggtt
      901 tggggtccat caatatccag ctcgctattc tttccatggc gaccggagat atcgtctgtt
      961 cgatctctcc atactcactg ggcgcgcccg tctttgcggt ttggaacaga tgattcaagt
     1021 tgggcagttc atcgacttca aactgttggt tgccgccctc ctcaagagcc tttctgatcg
     1081 cgggtagatt ctgtttcggc gaaacttgta ggtccctttc gccgttgatg gcgagtactg
     1141 gacatttcac tttccgcaac gccgtcgcgg ggtcgtaggt aatgaggtag cggatccatg
     1201 gagaggtgat cgccttgatc tggacgttga tctgcgcctc tgagcccacg cctacgagtt
     1261 tgtctcgcag ttctttctgc agcacagcat catctttttc ttgtttaacc aatgtaagga
     1321 gttcgcgctc cagagcaacg tttttctccg ccgcctcatg gctcacgcca ctggcttcgg
     1381 aaatcagcag tctctgctcg accaggatct cgtcgcccgg cacgcccgga ccagccatca
     1441 tcacgataaa agctacatcg ggatcggtgg ccgccaccat cggtgcgatg agtccgccct
     1501 cgctgtggcc gatcaagcca atcttgtgag gattcacctc agagcgcgtt ttgagatatg
     1561 cgactcccgc ttcggcgtca gtcgcgaagt ctgcggtagt agcatcggtc cgttccccgg
     1621 tggatttccc tattccgcgg tcgtctaccc gcagcacaac gattccttta cgtgttagaa
     1681 agtccgcaag cacgagaaac ggtttgtggc cgagcaaggt ttcgtctcga tcatggggcc
     1741 ccgatccggg aatcaagagc accgccggaa atggaccttt gccgggcgga accgtcatca
     1801 ttcccgcgag ggtgacacct cggactttat cgttgctata cgagacttcg tgttcgctat
     1861 atggatacgg cttaacgggg tcctgcggcc gggagcggag ttccaattcc gacacatgct
     1921 tgacgcgctt gactaccaga gacaggctgc cggcacgcca tgtatacgtc ccgtcaatgg
     1981 tcgataggtc tgagctgacc ttgccctcgt aggcggcgcc tttctgcttc atctcaatct
     2041 tgagagacga accattcctc gtgaccgccg ttgctagtac cccattcact ccctgatcgg
     2101 ggctatccat ggtcgcagtc aggccatccg ccgtattgag gatgtggaac acgacccgca
     2161 gctcgatacc gctggcctcg atcgtaccca cccaggcgcc atcaatatcg gaaggcgcgg
     2221 caggcttggg ctgggccttc acagcgtcgg tcgcgcgcct aaaatcaaga tccaggaatg
     2281 cccctccttg cgaccaggaa ccggagatct tcgtagcctc cggattaagc ttcccgacat
     2341 aagacccctg aaccttgtcc acatcgaact ctagctctga atttttcagc gagatggagc
     2401 tgacgggcag gccgaacgcg cgctgatcga caatgtccat ggtcgcctta agatttccgt
     2461 cattgtcctt tgtgacgtgg aggacgatgt gaagttgtgc cgtcccggca ttgagcaagc
     2521 cctggcaatc accagtaaca tcctggcttt gcgcgaggca tacggcagaa agaattaaag
     2581 cggcgattgc gataattttc ttgatgcttc gcctcccgca tagtacgtcc atgcacagag
     2641 cccggcgcaa gtacgttagc gctcatcggc ccctcatggc cgcactacct atctaacgaa
     2701 atctccccct gggctcccca cggcacgaga ctatataccc ctcccagggt caccgcacta
     2761 tatttcctgg ttggccggct ccaaaccacg aaccgggtcg gcatcaccgg aacgtactgg
     2821 tgactaatcg cactcacacc attacgagat aactcctgag tcgtcggcca acgggaactt
     2881 ggccagaacc gggatgtggg agattactcc cgaaaagcgt gcaactggcg tttgccagac
     2941 agggtgcaaa ggaacaggga aagagcgatg atgattggcg ccggtcggat gacagctaca
     3001 gcccgcgccg acactggcgc ttcagaagcc cgacgaaaag cgtgggtccg aggtttgcgt
     3061 acagacgtgt gatgacgctc gtcagctttg acgacgatct cgattgggag tttgcctttg
     3121 attcgcacct tgggcatccg gccggtcccc ggaggagcca atggcgaaga gatccaagaa
     3181 acggtgcgag gaattgccca tcttgcaccc ccatgcagcc ggcatcgatg taggtgcgag
     3241 cgagttgttt gtcgcagtca gcgctgatcg tgatccccaa cctgtccgca gtttcccaac
     3301 c
//
LOCUS       DASRKK010000021        13852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_391868, whole genome shotgun sequence.
ACCESSION   DASRKK010000021 DASRKK010000000
VERSION     DASRKK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391868"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(737..1240)
                     /locus_tag="VFC15_00295"
     CDS             complement(737..1240)
                     /locus_tag="VFC15_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08633.1"
                     /translation="MTEMPLMRCAGVTRTACLLAIVLAFIAPGLAQEQSDRPLTNSDV
                     VKMVNAGIPESVIVRDIEISGTNFVTTADALISLKQHHVPDSVLGAIVDSQAASKMRQ
                     AGPPAIAYAAAQSASHPHRQLPNVDATLRLDSKTTGKVQVRKNEIKVEKAGVPLFSVK
                     WKENPAK"
     gene            complement(1365..2555)
                     /locus_tag="VFC15_00300"
     CDS             complement(1365..2555)
                     /locus_tag="VFC15_00300"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZM08634.1"
                     /translation="MNEKIPKPMLNALAREAAPADHPSADVLAAFVEHALAKGEKQSV
                     TDHLARCEECREIVFLTSDATEEEVQEPAGKLVAAAALGEQDHPAVEQAAASIQRPFA
                     AAEPRWRSTSKWVWAVPVAAMFLLGAGYLLRKSYVAAPAERETASKTVPEKASGPPEQ
                     LPEATPALPAPAAAAPSPAAKGRPQMAPATTPPAQKAPTVNADVIAMNSAAPAPAGRA
                     TVHVKSAPPQPSPEAPVIAIGGAVAGTAPTAPRANGFAPTTGEIDRQYRAADSLNLSV
                     NRALAGAVRTAHPGWRVTLQGHLEHLMSEGWSRVLADQTSAFRAVSVIGNDVWAGGND
                     GMLFHSGDGGEHWNRLSIPSASGTETVTIVSIRFDDAQHGVVVADTGASYSTTDGGVT
                     WTKQ"
     gene            complement(2552..3469)
                     /locus_tag="VFC15_00305"
     CDS             complement(2552..3469)
                     /locus_tag="VFC15_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM08635.1"
                     /translation="MPLAPTSGPIEERPSAAVEEFARQHYVSSGAQQFGIDQAAFAAV
                     LTQVVLQTGPFGDDAAEMRFLTSLRVDELVLARACDDGNERAWETFLTRYRNTLYESA
                     YKIARTESAARSLADSLYAELYGVDSKGQQRTSKLSYYQGRGSLQGWLRVVIAQEYVN
                     QYRSVRRETSLDAALQDGAQFTAPEPEAAVADPRVEAAVAAELAGLDAEERFLLAAYY
                     LDHRTLAELAKLQGVHESTISRKLERITAGARKRVRKRLMQAGMSARQADEAMEDVDV
                     RDLRVRVSETLQQGRSKATFYKEKGEPQE"
     gene            3598..4686
                     /locus_tag="VFC15_00310"
     CDS             3598..4686
                     /locus_tag="VFC15_00310"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HZM08636.1"
                     /translation="MNSMILAGVASVLLTSALAGAGTPTHEYKVLPPITRGNLSLFPI
                     VGGTEANTAPLLTLDEGVRSGAVVVTEAGSLQGLVRPPTHYQRNTGGEVNRLVLVNNS
                     ARPLLLLAGEVVTGGKQDRVIGLDRIVPPKSDPVDLSVFCVEPGRWVVSTEHFGSMGS
                     AMAQPSVRMPAMAARDQLQVWKHVASANAGIANAAPQAAPAIGGTTSYAKVMQNPEVE
                     KKVASVAANYDGLLHELRKVGAKGVVVAVNGRITWADVFASTDLLEKYWQKLIRSYAA
                     ESLTDAGTGAQADQKSAQAFLDQLQGTREVSETEPGVFRRTEIIGEGYKVFTLNSLLP
                     KEEYTVHLAKMSYKEVMFPDVRPLERVR"
     gene            complement(4669..6423)
                     /locus_tag="VFC15_00315"
     CDS             complement(4669..6423)
                     /locus_tag="VFC15_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM08637.1"
                     /translation="MTQAAAIYARVSSDRQKEEHTIASQTAALMEYAQKHNYVVPPEW
                     VFQDEGYSGATLLRPGLEALRDLAAQGQMVAVLVYSPDRLSRKYAYQVLLAEEFARCG
                     VKLVFLQSPAGTTAEDQLVVQFQGMIAEYERAQIAERSRRGKRHKAQQGVVNVLSGAP
                     YGYHYVKKSEASAGYYQVVEAEAQVVRWVFEMYTQQQLSLNAMARQLNQRQILTRSGK
                     VGWRRQTIWHMLRNPAYRGTACYGKTGLQARPQGTRFLRQRKRSPHPDTVNRERPRNE
                     WIEVSVPALVDESVFALAQEQLQKNQHFSPRRTKRPCLLQGLLVCQQCGYAMYGTSGG
                     KPEQRLYYYRCQGADGYRWPQGTRCTNRPARQDYLDQVIWAQIIALLENEKLMQSEID
                     RRRETAGKTDPCERRKETLRSEQARLRNQMERLITAYQDGLLTLEQLRERMPTLKQQS
                     QAVNSELQLLEMEKLDQAKYLKLAESLGGFRNKLHARAQTLDVKERQQILRLLVKEIL
                     VGFDTLTIRHSIPIPSGDGPPSNVPGSTPSPQSDYPLRPRRVYAGPGLRKDEYRGFRR
                     LAGRRDPLESNSCLPHPL"
     gene            complement(7378..9291)
                     /locus_tag="VFC15_00320"
     CDS             complement(7378..9291)
                     /locus_tag="VFC15_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HZM08638.1"
                     /translation="MDILFAVSPFTDIEHPAIGVSLLQAQLKRSGFSSEICYFNLDLA
                     ERIGLRLYNWLSLCGDQPLLGTTAPAGSMIGEWFFADLVFGHQIPGEDEYTAEFLTPE
                     NRSHELIPQLLEARRSRREFIDQCVVEIQRHSPRVVGFTTSFHQTCACLAMAARLKES
                     DAPPIIIFGGANCAGEMGAQMLRSFPWLDYVCTGEGDEVLPRFLQRLLRDGDPSPVPG
                     ILQRGEEHALSLAPLVRDMDALPIPDYSDYFEKLRKSSLNGCIKPRLLIETSRGCWWG
                     EKHHCIFCGLNGETMAYRSKSPNRVIRELSYLCETHEVKRFDCVDNILDSRYIRTIFP
                     ALIENGPHIELFYATKSNLTFDQLRLLHLGGVRSIQPGLESFSTQVLRLMRKGCTGLQ
                     NIQFLRWSEELGISVAWNILYGFPGEDPSEYTLMAELIPLLTHLQPPAFCETARMDRF
                     SPMFTAPEQFGLVRRRPVTGYRYVYPLEGASLEKLAYYFEFEYGDGRQPETYVNDLIR
                     EVDSWANLRNQHRAQLDLYQAASGVLINDTRPCAVKQTHILFGVAAKVYLLCDTSRTA
                     ASVASKVGDVVSEHEISALFKKFVAAKLMIESDGHYLSLAVIRNRTHSAEIRDPLSEK
                     TVSVGSSSAQSHL"
     gene            9578..11827
                     /locus_tag="VFC15_00325"
     CDS             9578..11827
                     /locus_tag="VFC15_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM08639.1"
                     /translation="MINERLGHYHILEKIGEGGMGVVYRAHDERLRRDVAIKFLTAPR
                     LANPDARKRFRSEALALSRLNHPNIATVYDFNTQDGTDFLVMEYITGQSLNEKIAEGP
                     LPEKDIVRWGTQLAEGLAAAHEQGIVHRDLKPGNLRIRPDGKMKILDFGLATIMRISA
                     DDLTHTMTLTHEGGGTLPYMAPEQIYGQPVDARTDIYAVGAILYEMATGQRAFGTNDP
                     GQLIDCILYRSPKPPNSVNRRISPALESIIVKAIAREPRLRYQSARELLVDFERLSAG
                     VHPKAALTVQRRSSARRLSLAVIPFFNMTKDPEIDYLTDGVTESLIYRLSSQPRLRLM
                     ARTTVFRFKGTDVDPLEAGRSLGVRAVLTGKMAQRGSALNVQVELVNVANGTLLWGEQ
                     YDCDAADILNLQDGISAKIAEKLLLKLTREEQNALAKRYTEDNDAYQLYLRGRYYSDR
                     WTADVVDRGIPYYDQAIERDPAYALAYTGLAEAYCGMSSQFMSPREAMPKVRDAAIKA
                     LAIDNTLPEAHALLAVVRAFYEHNWAGAEAGFTRALELNSGSAAAHEWYGYYLTAIGS
                     EQEALNELALARELDPLSTKINLVLGLSFGMARRYEKAIEQLVKTITMDPDFWLGHLW
                     LGWAYEQMGRRDEALEELNKAFRLGASPYASAYLGYVQALAGRRSDAEVLVRAMEEQF
                     KHTYVSPHHIAIVYVGLGERDRAFEWLEKAYEHREEILVFLNVDPTWDSIRADARFRD
                     LVHRLGLPK"
     gene            complement(11873..12544)
                     /locus_tag="VFC15_00330"
     CDS             complement(11873..12544)
                     /locus_tag="VFC15_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZM08640.1"
                     /translation="MRRKIRLVSGGLNQAAHAFWSHAQLRELVPEFLFVDYSISRATT
                     PLMEAAFTVARNLSDSDPVAACLAEYLSKHIPEERHHDEWLLGDLEAIGQKREQVLER
                     MPSPTAATLVGAQYYWVFHAHAVSLLGYLAVMEGEPPSFLSLQEVIAKTGLPVKAFRT
                     FIEHAHLDPQHTDELNDVLDRMPLNERHKSLIGVSAVQTVHWLRCAFEELLEPHSQLQ
                     SVGGG"
     gene            complement(12579..12776)
                     /locus_tag="VFC15_00335"
     CDS             complement(12579..12776)
                     /locus_tag="VFC15_00335"
                     /inference="COORDINATES: protein motif:HMM:NF045560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aroma-sacti cluster domain-containing protein"
                     /protein_id="HZM08641.1"
                     /translation="MERTRAHVNVKKLQKAGILGTRQPLTPEDHEVIKSLSPDEVDAW
                     VRIKKKLDDAGCQEPECKFFI"
BASE COUNT         2982 a         4030 c         3930 g         2910 t
ORIGIN      
        1 acgtataacg cactagcgac cgaagttgcg gcgagcagtc ttcccgctct acaggccggg
       61 aggctcgtcg ccttcgaagt gcggatcgaa cccgccaaag aataagcact ccagccggtc
      121 cgcctcacag agaattctgc cgggcactcg ctttctctcc agccggagag actctgtcaa
      181 gccgtttatg gcgttggcga catctctgtg tatgagtgat catgtcgcgt caagtcgatg
      241 tagcttcggg ctgcctctgc gaacgaaagg aattctcgcg tcggatactt ccggtttgtt
      301 ccgccgagcg cctcctggaa agagcaccat cgatctacta cggtcttggc tacgcggttc
      361 ttctcgggat tgtccatgga atgctgcctt ggcgcccagc gacacaggcg cttgattcga
      421 ccgtcacctt tctcaatacc acagattgcc ggacaggacg cagggcgcgc cagccgcgcc
      481 ctgcccagat accgacttcc tgggcgcttt gctggtgtat tgcccgccgc gccggaggaa
      541 tcccagggat tgtcgggggc caacttccca gatgatgact tctggcccgc gtcgacgagc
      601 ggtagtgaac acgtcaactc acgacctgcc tcgaacgggc cgttcagccg gaattaccta
      661 cgacattcag atctcatcgc gaagtcggcc agcaggaatt tcggatgggc tatgatggcg
      721 caacatgacc agcgatctac ttcgcaggat tctctttcca tttcacactg aacagcggca
      781 cgcccgcctt ctctaccttg atctcgttct tcctgacttg tactttccct gtcgttttgg
      841 agtccaggcg gagagtggca tcgacattag gcaattggcg atgggggtgc gacgccgact
      901 gggcagcggc ataggctatc gccggcggtc cggcctggcg catcttagag gcagcctggc
      961 tgtcaacaat cgcacccagc acgctgtccg gcacgtgatg ttgcttcaag ctgatcaaag
     1021 cgtccgccgt ggtgacgaag ttcgtccctg aaatttcgat gtcgcgcacg ataacactct
     1081 ccgggatacc ggcattcacc atcttcacga catcagagtt cgtcagcgga cggtccgact
     1141 gctcctgcgc aagaccggga gcgataaaag ccagcacgat tgccaacagg caggctgttc
     1201 gggttacacc agcgcaacgc atgagcggca tctccgtcat tcaaaggatc tcgggctgcc
     1261 catatctagc acacgcctcc gtctgctcaa aacggctagc cttatctata gatagaacga
     1321 tgcccggcgg tcggcttgca gcaattattt cggggaggta acgatcactg cttggtccag
     1381 gtaacgccac cgtccgtggt gctgtagctc gcaccggtgt ctgcaacgac cacgccgtgt
     1441 tgcgcatcgt caaagcggat cgacacgatc gtcaccgttt cggtgccact tgcgctcggg
     1501 atggacagcc tattccagtg ctcgcccccg tcgccggagt gaaacaacat tccgtcattt
     1561 ccgcccgccc acacgtcgtt cccgatgacg ctaaccgcgc gaaaagcact cgtctgatcg
     1621 gccagcacac gtgaccaacc ttcggacatg agatgctcga gatggccttg cagcgtaacg
     1681 cgccagccgg gatgggcagt tcgcacagcc cccgccaggg cccggttgac ggacaggttc
     1741 agcgagtccg ccgcacggta ttgccgatca atttcacctg tcgtcggcgc aaagccgttg
     1801 gccctgggtg ctgttggggc cgtgcccgcc accgctccac caatcgctat tacgggcgcc
     1861 tcgggtgacg gctgcggcgg ggcagatttt acgtgcacgg tcgctctccc cgcaggagct
     1921 ggcgcggcgc tattcatcgc gatcacgtcg gcattcacgg tcggagcctt ctgggcaggt
     1981 ggggtcgtcg caggcgccat ctgcggccgc cccttcgctg ccggcgatgg agcagccgct
     2041 gccggcgcag gcagtgccgg agtcgcctcc ggcaactgct cgggagggcc gctcgctttc
     2101 tcaggaactg ttttggaagc agtttccctc tctgctggtg ccgcgacata gctcttccgc
     2161 aggagatatc cggcgccgag taagaacata gcagcaacag gaacggccca aacccatttg
     2221 ctcgtcgacc gccatcgcgg ctccgccgct gcaaacggac gctgtatgct cgcggctgct
     2281 tgctctaccg ccggatgatc ttgttcgccc agagctgccg ccgccaccaa tttgcctgct
     2341 ggttcttgca cttcttcctc agttgcatcg ctcgtcagga aaacgatctc gcggcattcc
     2401 tcgcaccggg ccagatggtc ggtcaccgac tgcttctcac ctttggccag tgcatgctcc
     2461 acgaaggcag caagcacatc cgcagaagga tggtctgcgg gcgccgcctc tcgggcgagg
     2521 gcattgagca tcggcttggg aatcttctcg ttcattcctg aggttcgccc ttctccttat
     2581 agaacgtggc tttcgacctt ccttgctgca aagtttcact cacccgcact cgtagatcgc
     2641 ggacatctac atcttccatc gcctcgtcgg cctggcgcgc ggacatgccg gcctgcatca
     2701 accgcttacg gacacgtttg cgtgcgcccg ccgtaatgcg ctcaagcttg cggctgatcg
     2761 tggattcgtg cactccctgc aacttggcca gctccgccag ggtccggtgg tcgagatagt
     2821 aggcggccaa aagaaaccgc tcttccgcat cgagaccagc cagctccgcc gctaccgccg
     2881 cttcaactcg gggatcagcc accgcagctt ccggctcggg cgcggtaaac tgtgcaccgt
     2941 cttgcagcgc agcatcgaga cttgtctcac ggcggacact gcgatattga ttgacgtact
     3001 cctgcgcgat gacaacgcgc agccacccct gcaacgaacc tcgcccctgg taatacgaca
     3061 acttcgaggt ccgctgctgg cccttggaat ccactccata gagctcggcg taaagcgaat
     3121 ccgcgaggct gcgagccgcg ctctccgtcc gcgcgatctt atatgccgat tcgtacagcg
     3181 tattgcggta gcgcgtaagg aatgtctccc aggcgcgttc attgccatca tcgcacgcac
     3241 gggcaaggac cagctcgtcc actcgaagtg aggtcaggaa acgcatctcg gccgcatcgt
     3301 cgccgaaagg acctgtctgc aagacaacct gcgtcagcac cgccgcaaat gccgcctggt
     3361 cgatgccgaa ctgctgtgcc ccactggaga cgtagtgctg gcgggcgaac tcctccaccg
     3421 cagcggacgg ccgctcttcg atcgggccag aagtgggagc caggggcatg tgtgcgaatc
     3481 gtatcaattc gtgcgcaaga agaacacagg gcggcgttct accgctagag acgtacaaac
     3541 acctcagctg taagccgtga ctggctgcgg aagagaggtg cgcctcggga ggagaacatg
     3601 aacagcatga ttctggccgg cgttgcttcc gttttgctga cttccgccct tgccggcgcg
     3661 ggcacgccaa ctcacgagta caaggtattg ccacccatca cccggggaaa cctcagcttg
     3721 tttcccattg tcggcggcac ggaagccaat actgcgcctc tcttgacgct cgacgaagga
     3781 gtgcgctcgg gcgcggtcgt ggtcacagag gccggctcgc tacagggact cgttcgtccc
     3841 cccacgcatt accagcgcaa caccggaggc gaagtcaatc gcctggtgct ggtcaacaac
     3901 tccgcccgcc cgctgctgct gctcgccgga gaagtggtga ccggtggcaa gcaggaccgc
     3961 gtcatcggcc tcgaccgcat cgttccgccg aaatccgacc ccgttgatct ttctgtcttc
     4021 tgtgtcgaac cgggacgctg ggtggtgtcc acggaacact tcggcagcat gggttcggcc
     4081 atggcacagc ccagcgtgcg catgcccgcg atggcagcgc gtgatcagct gcaggtgtgg
     4141 aagcacgtgg catcagcgaa cgctggaatc gctaatgcag cgccgcaagc tgctccggca
     4201 attggcggga ccacttccta tgccaaggtc atgcagaatc cggaggtgga gaaaaaggtt
     4261 gcctcggttg cagccaatta cgacggcctc ttgcacgaat tgcgcaaagt aggtgccaag
     4321 ggagtagtag tggccgttaa cggtcgcatc acctgggccg atgtgttcgc cagcaccgac
     4381 ctgctggaga aatactggca aaagctgatt cgctcctacg ccgccgaatc gttgaccgac
     4441 gccgggaccg gcgctcaggc cgaccagaaa tcggcacagg cgtttctcga tcaactgcag
     4501 ggaacacgcg aagtcagcga aaccgaaccc ggagtcttcc gacgcaccga gatcattggc
     4561 gaaggctaca aggtgtttac cctgaattcg ctcctgccta aggaagagta cacggtgcat
     4621 ttggccaaaa tgtcgtacaa agaagtcatg tttccggatg tgcgcccgtt agagcgggtg
     4681 cggtaaacag gagttgcttt cgagtggatc ccggcgcccg gcgagacgtc ggaacccccg
     4741 gtactcgtct tttcgaaggc caggaccggc gtatacacgc ctgggacgca aagggtaatc
     4801 ggactggggg gagggggtag aaccaggaac gttggatgga ggtccgtcgc cggaaggaat
     4861 cggaatcgaa tggcggatgg taagagtgtc aaagccaacc aggatttctt tcacgagcaa
     4921 gcgcaagatc tgttggcgct ccttcacatc cagggtctgc gcacgtgcat gcagtttgtt
     4981 gcgaaaccca cccaggcttt cggctagctt cagatacttg gcttggtcta gcttctccat
     5041 ctccagcaat tgtaattctg agttcaccgc ttgcgattgc tgcttgagag tgggcattct
     5101 ctcacgcagt tgttccaggg tgagcagccc gtcttgatag gcagtgatta agcgttccat
     5161 ctggttgcgc aaacgtgctt gctcactacg gagagtttct ttacgtcgct cgcagggatc
     5221 cgttttgccg gccgtttctc tgcggcgatc aatctcggac tgcatgagct tctcattttc
     5281 cagcaaggcg atgatttgcg cccagatgac ctggtccaga taatcttgcc gcgccggacg
     5341 gttggtgcac cgtgtacctt ggggccagcg atatccatcc gcaccctggc atcggtagta
     5401 atacaaccgc tgctcgggct ttcctcccga agtcccgtac atcgcatagc cacactgctg
     5461 gcacaccaaa agtccctgca gtaagcaagg ccttttggtg cgtcgcgggg aaaaatgttg
     5521 gttcttttgc agctgttctt gcgccaaggc aaacaccgat tcatcgacca gggcggggac
     5581 gctcacctcg atccactcat tcctgggacg ctcacggttc accgtgtcgg gatgcggcga
     5641 acgctttcgc tgccgcagaa accgagtgcc ctgcggtcgc gcttgtaggc ccgttttgcc
     5701 ataacaagcc gttcctcggt aagccggatt gcgcagcata tgccatatcg tttggcgccg
     5761 ccagcccacc tttccgctgc gagtgagaat ctggcgttga ttcagctgtc gagccatggc
     5821 attcaggctg agctgttgtt gggtgtacat ctcaaacacc cagcgcacca cctgagcttc
     5881 cgcttccact acttggtaat aacccgccga ggcttcgctc ttcttcacgt agtggtagcc
     5941 gtaaggggcc ccggaaagca cattcacgac tccctgctgc gctttgtgcc gttttccgcg
     6001 gcgactccgt tcggcgattt gggcacgctc atattccgct atcatccctt gaaactgcac
     6061 caccagttga tcctcggctg tggtcccggc tggggattgg agaaaaacta atttcacacc
     6121 gcaacgggcg aactcttctg ccagcaaaac ctgataggca tacttgcggc tcagccggtc
     6181 cggcgaatac accagaaccg ccaccatctg gccttgcgct gccaagtcac gcaacgcctc
     6241 taatccgggc cgtagcaagg tagcgccgct atagccttcg tcttgaaata cccattccgg
     6301 cggtacgacg tagttgtgct tctgggcgta ctccatgagg gccgctgtct gactggcaat
     6361 cgtgtgttcc tccttctggc gatcggaaga cacccgggcg tagatggccg cagcttgcgt
     6421 catgcaaatt ctcctgaggc aaagtaggga ccggacaacc aatcgggcgg gcatggtcgg
     6481 ggaccaacag ctgataaacc tgttccagct tatccggcaa caaacgatca aatagatatt
     6541 ggaactgaag cttgcagcgg aggggacgcc gctcagccac gccctcgtct ccgcgttagc
     6601 agcgccacta acgcccggct cacgaactcg ctcaaggaca aaccggcctc ctgggcacga
     6661 gtccgaactt gcctcaccaa ttggcgagaa agcttcacgg tgaaagcgac tctggtatcg
     6721 ctgaccggaa gcggctgctt cagatgccga gcttgttgca gataacgata ggcctggcgc
     6781 ggcgagaggg cgctgtcccg caccatacgg gagacggcgt cgggcaaatg ctgaaaccgc
     6841 tgcagcaggt cgcgagcatg attgagacgt ttggttttga gcccttgact cgcgcggggc
     6901 atatgtcaca ttaatacaac tgcatatgac agatatcaag tagccagcag gtatcatatg
     6961 cggtacccaa ttcggcgttc aaattcgaga ttatcgtttg cgttccagga acctacacac
     7021 ccgcgacaat gtgacagaaa cggtatcgtc cggagtcggc cgctctcgga ccagagaccc
     7081 ccaaacgatg tacatagagc tcaataactc gtagatcagc gatattttga ctcagaagga
     7141 agcgatcaaa tggctaccgc aaccaagcga acccgcaaac acttcagttg gattcagcaa
     7201 agatcaagcg cgcccaaaga gcgttgcgtg cgagaacgga gaccgaggcc atcgagcgcg
     7261 ctctagcact ggtctgcgtc ggagcgcaca agaccgcagt ggtatatcgc ctgtttgcga
     7321 cagcatcgcc gacacatggc gttggcgccc cggttgccga aaagcgaatg gccctaatca
     7381 taagtgggat tgagcagagc tggagccgac tgagactgtt ttctccgaaa gtggatcgcg
     7441 gatttcagcc gaatgggtac gattgcggat gacagcaagg ctcaagtagt ggccgtccga
     7501 ctctatcatc aatttagcgg cgacaaactt tttgaaaaga gcggagattt catgctcgct
     7561 gacgacatcg cctaccttgg aagccacgct cgcagcggtt cgggacgtat cgcaaagcag
     7621 atagactttg gcggcgacgc cgaaaagaat atgagtttgc ttcaccgcgc aagggcgcgt
     7681 gtcgttgatg aggacccccg atgcggcttg gtaaagatcg agctgcgccc gatgctgatt
     7741 gcgcaagttt gcccagctat cgacctcgcg gatcaaatcg ttgacgtacg tttcgggttg
     7801 ccggccatcg ccgtactcaa attcgaagta gtaggcaagc ttttcaaggg acgccccttc
     7861 cagcgggtac acatatctgt agccagttac gggacgccgt cgcacaagtc cgaattgttc
     7921 tggggccgtg aacatcgggc tgaaacgatc cattcgtgct gtttcgcaaa atgccggcgg
     7981 ctgaagatgc gtcaataacg gaattaactc cgccatcaac gtgtactcgg atggatcctc
     8041 gcctgggaat ccgtacagga tattccaggc aacagaaatg ccgagttcct cagaccagcg
     8101 caagaactga atgttctgca gcccggtaca ccccttccgc attaagcgaa gcacctgcgt
     8161 gctaaagctt tcaaggccag gctggatgga gcgcacacca ccaagatgga gcaaccgaag
     8221 ctgatcgaag gtaaggttcg actttgtcgc gtagaagagc tctatgtgcg ggccattttc
     8281 aatcagtgca ggaaaaatcg tccggatgta tcgggaatcg agtatgttgt cgacacagtc
     8341 gaagcgcttg acctcgtggg tctcacagag atacgacagt tcccggataa ctcgattcgg
     8401 cgacttgctc cggtacgcca tcgtctcgcc attcaacccg cagaaaatgc aatggtgctt
     8461 ttctccccac cagcaaccgc gggaagtctc aatgagcagg cgcggcttga tgcagccatt
     8521 cagcgacgat ttgcgcagct tttcgaagta atcggagtaa tccgggattg gaagtgcatc
     8581 catgtccctc accagaggag cgagacttag tgcgtgttcc tctcctcgtt gcaggatgcc
     8641 gggcaccggg ctcgggtcgc cgtcgcggag cagacgctgg agaaagcgag gcagcacttc
     8701 gtctccctca ccggtgcaga cgtagtcgag ccagggaaac gaacggagca tctgggcccc
     8761 catctctcct gcgcagttcg cgccaccgaa gataatgatc gggggggcgt cggactcttt
     8821 cagacgggcg gccatagcca gacacgcgca cgtttgatga aaagaagtgg taaagccgac
     8881 cacacgcggc gagtgtcgtt ggatttcaac gacacactgg tcaatgaatt cgcggcggct
     8941 acggcgtgcc tccaaaagct ggggaatgag ttcgtggctt ctgttctccg gcgttagaaa
     9001 ctccgcagtg tattcgtcct ctcctggaat ctggtgacca aagacgaggt cggcaaagaa
     9061 ccattctccg atcattgacc cagcgggagc agtcgtaccg agcaggggtt ggtccccgca
     9121 tagggagagc cagttgtaga gccgaagacc tattcgttca gccagatcca aattgaaata
     9181 gcagatttcc gaagaaaagc cggagcgctt cagctgcgcc tgtaacagac ttaccccaat
     9241 cgcgggatgc tcaatgtcag tgaagggaga gaccgcaaac aagatgtcca ttgggttctg
     9301 actcgccaca gccgcgtcgc ggatctccga aacgctgggc cgaacattcg aatgccagca
     9361 gatcctggcg aacaactcgt tgttccgtac tgcgaagacc gcacttcggt gtgatcgatt
     9421 gcaaccaacg acccggctat cctacaccgg cagtttacgt gagtccaaaa atgccatccg
     9481 cagcacttgg cgtccaatgt tacttctagt cacgcccagc ttcaagggtt gacttcgaca
     9541 tgccgcagcc ctacagtaca gcgcaggttc gctcctcatg atcaacgaaa gactaggcca
     9601 ctaccacata ctcgaaaaga ttggtgaggg ggggatgggc gtggtgtatc gtgcgcatga
     9661 cgagcggctc aggcgtgatg tggcgatcaa gtttctcacc gcccccaggc tggccaaccc
     9721 agatgcccgg aagcgatttc gaagcgaggc attggcgctc tccagactga atcatcccaa
     9781 tatcgcaacc gtgtatgact tcaataccca ggatggaaca gacttcctcg ttatggagta
     9841 catcacgggg caaagcttga acgagaagat agccgagggt ccattgcccg aaaaggacat
     9901 cgtgcgctgg ggaacccagt tagcggaggg gttggcagca gcgcatgagc aggggatcgt
     9961 gcatcgcgat ctgaaacccg gaaatctccg catccggccc gacggcaaaa tgaagatttt
    10021 ggattttggt ctcgcaacaa taatgaggat atccgcggac gacctcacgc acactatgac
    10081 gctgactcat gaaggggggg gcacgctgcc ttatatggct cctgaacaaa tctatggcca
    10141 gcctgtggac gcgcgcaccg acatctatgc agttggtgcg atcctctacg aaatggcgac
    10201 ggggcagcga gcgttcggga cgaacgatcc agggcaatta atcgactgca tattgtaccg
    10261 ttctccgaag ccaccgaact ccgtgaaccg gcggatttcg ccggcgctcg agagcatcat
    10321 cgtgaaagcg atcgctcgcg aaccacggct acgataccag tctgcacgcg agctgctggt
    10381 tgactttgag cggctgagcg ccggtgtcca cccaaaagcc gcgttgactg tgcaacgtcg
    10441 ctcatccgcg cggcgtctgt cgctcgcggt cattcccttc ttcaatatga cgaaggaccc
    10501 ggagattgac tacctcactg acggcgtcac agaaagcctt atctacaggc tgtcatcaca
    10561 gcccagattg cgcctgatgg cccggacgac cgttttccgc ttcaaaggca ccgatgtcga
    10621 ccctctggag gctggccgta gtctaggagt tcgcgccgtg ctgaccggaa agatggcgca
    10681 acgaggatcg gctctcaacg tccaggtcga gttggtaaac gtcgccaacg gtaccctgct
    10741 ctggggcgag caatacgatt gcgacgccgc cgatatcctc aacctccagg atgggatctc
    10801 cgccaagatc gcagagaagc ttctgcttaa gctcactcgc gaggaacaga atgccttagc
    10861 caagcggtac acggaagata acgacgccta ccaactttac ctgcgggggc gctactactc
    10921 cgaccgctgg actgcagacg tggtggatcg tggtattccc tattacgacc aggccatcga
    10981 aagagatccg gcttacgcac tcgcttacac aggtctagcg gaagcctact gcggcatgtc
    11041 cagtcagttc atgtctcccc gagaagcaat gcccaaggtg cgcgacgcag ccattaaggc
    11101 tttggccatc gataatacgc ttcccgaagc tcatgcctta cttgccgttg tgagggcttt
    11161 ttatgagcac aactgggctg gcgcagaggc cggattcacc cgggccttag agctgaattc
    11221 tgggtcggcg gcggctcacg aatggtacgg atactattta acagcgatcg ggtccgagca
    11281 agaggctctc aacgagttgg cgctggcgcg ggaactcgat ccactttcca ccaagattaa
    11341 tctcgtcttg gggctatcgt ttgggatggc gcggcgatac gagaaggcca ttgagcaact
    11401 ggtaaagacg atcacgatgg acccggactt ctggttaggt cacctgtggc tcggttgggc
    11461 ttatgagcag atgggaaggc gcgatgaggc gttggaggag ttgaataagg cttttcgatt
    11521 gggcgcaagt ccatacgcct cggcttatct cggatacgtg caggctttag ccggcagaag
    11581 atccgatgcc gaggtgcttg ttcgtgccat ggaagaacag ttcaagcaca cgtatgtgtc
    11641 gccccaccat atcgcgatcg tctatgtagg gctcggggag agagatcggg cgttcgagtg
    11701 gcttgagaag gcttacgagc accgggagga gattctggtg ttccttaatg tcgatccgac
    11761 atgggacagt atccgcgcgg atgctcgctt ccgagacctg gtgcaccgcc tgggtcttcc
    11821 caaatagata aaaccgtgat ggttcgtgtg ggcgcgagtg tgccgaatga tgctatccgc
    11881 ctccgaccga ctgcaactga gagtgcggtt ccagaagctc ctcaaacgcg caacgaagcc
    11941 aatgaaccgt ttgtacggcg ctgacgccaa tcagggactt gtgtctttcg ttcaatggca
    12001 tccgatccag aacgtcgttc aactcgtccg tgtgttgcgg gtcgaggtga gcgtgctcga
    12061 taaatgttct gaaagccttg accggaaggc cagttttggc aattacttct tgcagggaca
    12121 agaatgatgg cggttcgcct tccataacag caagatagcc gagcaaactc acggcatggg
    12181 catggaatac ccagtagtac tgcgctccta cgagcgtagc ggcagtagga gatggcatac
    12241 gctccagcac ctgttcccgc ttctggccaa tagcttccaa gtcgcccagc agccactcat
    12301 cgtggtgccg ttcctcaggg atgtgcttgg acaggtattc cgctaaacag gccgccactg
    12361 gatcggagtc cgacagattg cgcgcgaccg taaaagcagc ctccatcagc ggcgtggtgg
    12421 cacggcttat cgagtaatca acgaagagaa actccggaac aagctcgcgg agttgggcat
    12481 gagaccagaa agcgtgagct gcctgattca gcccaccgct caccaaacga atcttcctgc
    12541 gcaattgttc actattccga atggttctgc cctttggcct aaatgaagaa tttacattcc
    12601 ggttcctggc agccggcatc gtccagcttc ttcttgatgc gcacccaggc gtcaacctca
    12661 tcgggggaca aagacttaat cacttcgtgg tcctccgggg taagtggttg acgagttccc
    12721 aaaattccag ccttctgcaa cttcttcacg ttaacgtgag ctctcgttcg ctccactttc
    12781 ggctccttat gttttcttat tcccgatagc tccctagcaa atgacgccag cctaaagggc
    12841 gcaagcaatc taccagggag ttaacatact actatccaat tcgagtttgt caaagatgtt
    12901 attttagcga tggatatcag gtccgaaggt cgactcttct gttacccaga atgttagggg
    12961 aatggtgatg tccctctcgc acgcgcttgt tttcgtggac cgtaggcacc agattgacta
    13021 tcccaagcgt gtaccaagcg gctttttcca aaaaagccca attcgaattt atgtccaatc
    13081 ctgacagcgg ctccggcatt cctaagctcc caagccgatc gccctttaaa cttcgatcgt
    13141 gaccctatcg tgatcggaaa ttctgaacgg cgaagtggca gccagtaagc cttttataat
    13201 cttggcgtcc ccgacgggat ttgaacccgt gttaccgccg tgaaagggcg ttaacctatc
    13261 gcaaacacag gagcttatcg ggcatcggtc gctctgcatg taccgcaaga gccttttaag
    13321 aataccaagg cacaggtact ccttgtactg tctttgtacc gcgagaattc cgaaacagcg
    13381 aggatcgcca agctgtcctg caccggaacg aatcgggggt gtttggcacg cactggcttg
    13441 tatacacctc tgctggcaaa ggattccgaa actgaggggc cagaagtcgg cttgcaagag
    13501 ttccgccaga tcggtactcg taaacgccca gtgtttactg atttccagtg ggtgactctc
    13561 cacttggacg aatagtcgcc atgtcggaca agtgaattgt gaagacattc acatctccgg
    13621 gtagggcgtt ttacgtccaa gtggcggtgc caatgcggtt gccggacgct ggccgactta
    13681 ccaggagttg agattttgga aggttcgatg acgcgtcaat gagcgatcaa tccggcggat
    13741 ataggcttga acggaatacc gcacgtgagc ccggcacacc tcacgtcatc gcgggcgatg
    13801 gtcattaagc cgatactcgc cgaggagatc agcaagaatc catgctgttc cg
//
LOCUS       DASRKK010000022        21205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1173741, whole genome shotgun sequence.
ACCESSION   DASRKK010000022 DASRKK010000000
VERSION     DASRKK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21205
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173741"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFC15_00340"
     CDS             complement(<1..493)
                     /locus_tag="VFC15_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08642.1"
                     /translation="MGKCFRSADVNQALLLPPSLHDWLPEKHLARFLVEVVDALDLGA
                     IYESYEEKDGRGQAAYEPRMMVRVLLYGYCVGSYSSRKIAAKTYEDIGFRYLAADEHP
                     DHSTLAEFRKRHLKALAGLFCQALQLGEKAGLVKLGHVAIDGSKMQGNASKHKAMSYE
                     RMRE"
     gene            complement(582..1295)
                     /locus_tag="VFC15_00345"
     CDS             complement(582..1295)
                     /locus_tag="VFC15_00345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZM08643.1"
                     /translation="MTRRRWIADEVTHDRAALTGEHAAHLSRTLRARVGQEFEVVCDG
                     QVRHATVASVSDERVEFALGEEIAASVAAPITLLLAVFKFDRMEWAIEKCTELNVTSI
                     VPVIARRTEKHLALAADKRVERWRRIAREASEQSRRVAPPEIGDPVKLPTALETPAEL
                     RVVLAEAEKEAQLSEILRGRPELKSLAMAIGPEGGWMPEELQLFETAGWIAASLGDNI
                     LRAETAAIAALAVAQAEMC"
     gene            complement(1292..1675)
                     /locus_tag="VFC15_00350"
     CDS             complement(1292..1675)
                     /locus_tag="VFC15_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08644.1"
                     /translation="MDKTLRIYAWFLLIVTALPAFARLARPRDLARILLLRYQNPTRR
                     KRARIGGAIYLGLALLATPFLLRSPMQQQHWLVIALLVGAVSALEFLMNSRIFDEEAL
                     TRENRYFGGVYAAIAIAVALLLITR"
     gene            complement(1716..2861)
                     /gene="dnaJ"
                     /locus_tag="VFC15_00355"
     CDS             complement(1716..2861)
                     /gene="dnaJ"
                     /locus_tag="VFC15_00355"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZM08645.1"
                     /translation="MARETTLATKEKRDYYEILGVGRTATDQELKSAYRKQAMQCHPD
                     RNPDNPEAEEQFKECSEAYAVLADAEKRARYDRFGHAGVSGNGGGGFDPSGFPDIQDI
                     FGDIFGFGDIFGGGRGGRRSRAQRGADMREDLTLEFEEAVFGVTKQVQIRRLEECDQC
                     KGSGAAPGKAPIDCTTCGGRGQVRYQQGFFAIARPCGTCHGSGKLIIDPCPKCKGQQR
                     LRRERNVTVKVPAGVEEGARMLYSGEGEAGIYNGPPGDLYVVLHCKEHEFFEREGKDL
                     RCVVPVSVVQAALGTEIAVPTLEGEHTLKVPEGTQTGTTFKLRNKGVPELNGRGRGDL
                     YVELRVQTPAKLSKRQRELLQELQSTLSVENKPVSRTLLGKMRDIFN"
     gene            complement(2876..3457)
                     /gene="grpE"
                     /locus_tag="VFC15_00360"
     CDS             complement(2876..3457)
                     /gene="grpE"
                     /locus_tag="VFC15_00360"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZM08646.1"
                     /translation="MAMNKKNGEASELDLEHELPSAESEDAIPSRSRGGGATAADPEA
                     ELETLREERAGYLDRLARLQAEFDNFRKRSVKEQQDFRDYALAEALKSLLPILDSLDR
                     ALKTNATSVQEYLSGIELIDKQFHDALSRLGVEPIPAEGEPFDPNLHQAVQMVDTAEV
                     ADNHVIDELQRGYRIKDRLLRPAMVRVAHNPKH"
     gene            complement(3466..4515)
                     /gene="hrcA"
                     /locus_tag="VFC15_00365"
     CDS             complement(3466..4515)
                     /gene="hrcA"
                     /locus_tag="VFC15_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZM08647.1"
                     /translation="MGSPPGIGKREREILTAIVETYISTGEPVGSRTIARSNRESLSP
                     ASIRNVMSDLADAGFLEQPHASAGRIPTPQAYRFYVKQLTGEARLSPADESLIQGNLQ
                     GSSDVQEFMERTSHVLSLISHGVGVTVTSAHGQRNELEHVYFQRQGDKKVLAVVVMRN
                     GLVRDRVLRLTDDLQASDLEIAASYINQNFRGWQMEAVRNEIAKRVQQERTEYDRLMN
                     SVEQLYTQGALATEAAQQTVFVEGASNLVLNEQDRERLRQLLATLEERQRIVDLLSAY
                     LDARQEAVRVVVGLEDAVPHLKNFVLIGTPARVGDEVVGSLAVIGPTRLDYEHTITAV
                     SYIARMFDQFWNEST"
     gene            complement(5731..7692)
                     /locus_tag="VFC15_00370"
     CDS             complement(5731..7692)
                     /locus_tag="VFC15_00370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM08648.1"
                     /translation="MTLRKLLIGTIFLGLCATGLAQEATIAPVAITPNDALILENIPP
                     IPAGIAARADRYTQFRSATMWGWHPQRREILIGTRFGDTNQVHQVAMPGGARTQLTFF
                     PDRIADASYHPHSADYFLFRKDVGGGEWFQIFRFDVATGESTLLTDGKSRNSEFAWSN
                     KGDHIAYASTRRNNADLDLFVMDPTKKDSDKMIAQNQGGGWQVSDWSPDDRTLLVFNG
                     VSINESYLWLVDAASGQKTELTPKGSDKVSYTPIGFSRDGKGIYLTTDKDNEYQRLAY
                     MDLATKSLKYLTTYKWDIEDGAQLSEDHRHIAFVLNENGMSTLHVLDVDSGKELRIPK
                     LPAGVITNLRWHEDNRDLAFSLNTSRSPSDVYSVDIQTAKLDRWTMSETGGIQPQNLV
                     EPKLITWKSFDGKEISGWLYLPKDKPAAGKYPVEIVIHGGPEGQSRPTFLGRTNYLPN
                     EMGVALIFPNVRGSTGYGKAFTQLDNGFLRENTYKDIGALLDWIATQPDLDAKKVLVT
                     GGSYGGHMTLAVATLYNDRIACSVDIVGISNLVTFLEHTEAYRRDLRRVEYGDERDPK
                     MRAYLESIAPMNHVKAINKPMMVVAGVNDPRVPKSEADQMVKALETNGTTVWYLAAKD
                     EGHGFAKKKNADFQFYTTVLFTQKYLLGQ"
     gene            7895..8095
                     /locus_tag="VFC15_00375"
     CDS             7895..8095
                     /locus_tag="VFC15_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08649.1"
                     /translation="MAGSSRTTFTKRQKERARQERQAEKAQKKAQRKQEPNSPTEAPA
                     ELVITHDEEGQPLGFDFHDFGK"
     gene            8368..9549
                     /locus_tag="VFC15_00380"
     CDS             8368..9549
                     /locus_tag="VFC15_00380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZM08650.1"
                     /translation="MSLSAGLQQKLAAAHFTTPTPVQEMAIPHALEGKDVLATAQTGT
                     GKTLAFLLPIMEKLGAKHLPGVRALILVPTRELAIQVQAVYDKLRFGKLPKTALVIGG
                     MAERPQIEAVRKGAGVIVATPGRLEDYLKRRLVDLSKVETLVLDESDRMLDMGFLPAL
                     RRIVGSLPKQRQTLFFSATLEASVAGLVNDYMRNPVRVALGSISKPVESVELHAFEVP
                     AADKSDTLRRLLYDEQGQTLIFARTKRGTERLAKNLIRDGFSAAMIHGDRTQSQRVNA
                     LNGFGAGTYKVLVATDVASRGIHVDNVAHVINYDLPDLAEDFIHRVGRTGRAGLKGRA
                     STLVSGAEIGELRAIERTLKLKIARKRVDGSEIAAMKPRIVKNTLDSRTLAQLPGEVF
                     C"
     gene            complement(9814..10233)
                     /locus_tag="VFC15_00385"
     CDS             complement(9814..10233)
                     /locus_tag="VFC15_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM08651.1"
                     /translation="MTLAPSQLRAFAERYTAAWCSQDPASIAAYFAPNGSLSVNGVPA
                     VGRDAIAEVAHGFMTAFPDMLLTALDITTANGHVFYRWMLDGTNTGPGGTGKRVRIIG
                     FEKWTFGADSLIAESQGHFDQAEYQRQLKYGFDEKQR"
     gene            complement(10237..11082)
                     /locus_tag="VFC15_00390"
     CDS             complement(10237..11082)
                     /locus_tag="VFC15_00390"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZM08652.1"
                     /translation="MSSHTHSHGPEPAGPQPLPGVDAIIAVGSGKGGVGKTTLAVNLS
                     IALARMGFKVGLLDADVYGPNVPTMLGTTVAPEVVKGDRILPPERYGLKVISVGFLSP
                     GDKPLVWRGPMLHSIMKQFLGQVEWGQLDYLIIDLPPGTGDVVISLVQTVPVTGAVVV
                     TTPSDVALQDARKAIEMFKQVKVDILGVVENMGAFLCPHCQHEIDIFSKGGGERTAQQ
                     FGVPFLGSIEIDPDIRKGGDTGQPVVLEGDQSPHARSLFKFARHVVDRVSEVKTTSGP
                     VIEIQ"
     gene            11457..12428
                     /gene="cysK"
                     /locus_tag="VFC15_00395"
     CDS             11457..12428
                     /gene="cysK"
                     /locus_tag="VFC15_00395"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZM08653.1"
                     /translation="MKIANDVTELIGNTPLVRLNRVTKGVGALIVAKLESFNPCASVK
                     DRIGVSMISEAEKAGKIHPGRTTLIEPTSGNTGIGLAFVAAVKGYKLIIVMPDTMSTE
                     RRVLLRAFGAELVLTPGPKGMKGAISKAQEILAKTPDGFIPQQFDNPANPKIHFETTG
                     PEIWNDTDGKVDILISGVGTGGTITGVSEYIKPRKPSFKAIAVEPVESPVLSGGQPSP
                     HKIQGIGAGFVPSILRTDYIDEIVQVSSDDALVMARRLAVEEGLLMGISSGAAVVAAI
                     RVGERPENAGKMIVVVLPSFGERYLSSILFQQLRDEVVAQPTEPVSV"
     gene            12442..13149
                     /gene="cysE"
                     /locus_tag="VFC15_00400"
     CDS             12442..13149
                     /gene="cysE"
                     /locus_tag="VFC15_00400"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZM08654.1"
                     /translation="MVSYFRLMREDIAAVLERDPAARSKLEIVLLYSGLHALWWYRIH
                     HWLWKHGWLFLARALSQFARWLTGIEIHPGAKIGRRFFIDHGMGVVIGETAVVGDDVT
                     IYQGVTLGGTSLNHGKRHPTIGDRVVIGGGAKILGNITVGENCRIGAGSVVLRDVPAD
                     STVVGVPGHIVFRQGKRVVITDPKQINDPLSEALAAVANEVKTLRDQVCRLEGREHVP
                     AEGGDSLDLAIEMDYQI"
     gene            13229..13705
                     /locus_tag="VFC15_00405"
     CDS             13229..13705
                     /locus_tag="VFC15_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08655.1"
                     /translation="MRIELLHAKLRPSSLGGVAAGLTIYAPAACLFCLYFLLLPLAMN
                     AGTIEHPGILHKEDNCSSCHVDKTRGKSVHSAMEVPCTVCHLAETQGDMTTLRLSLPK
                     AEICFSCHEKTMLLRQHIEGGKKLCVDCHDAHSSNRRMLLLDPLNANRRNSSVMLK"
     gene            complement(13721..14452)
                     /locus_tag="VFC15_00410"
     CDS             complement(13721..14452)
                     /locus_tag="VFC15_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZM08656.1"
                     /translation="MTSPFMGEFAGTLVLILLGDGVVANVLLKRSKAEGAGWMVITTG
                     WAFAVLCGIFTAVAFGSADAHINPAVTLAFAITSGSFAKIVPYWSAQFLGAFTGATLV
                     WLYFLPHWKMTEDRDAKLAVFCTAPAIRHYPGNIFCEVIGTMLLIIVVVAIGSKAVSP
                     TGLTPGLGPYFVSALVWAIGLSLGGTTGYAINPARDFGPRVAHALWPVAGKGTSDWAY
                     APIPILGPLLGAALAAVIVKAAHIV"
     gene            complement(14486..16003)
                     /gene="glpK"
                     /locus_tag="VFC15_00415"
     CDS             complement(14486..16003)
                     /gene="glpK"
                     /locus_tag="VFC15_00415"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZM08657.1"
                     /translation="MPDYVGAIDQGTTSTRFIVFSRSGQVVATAQEEHEQLYPRPGWV
                     EHNPQEIWTRTQEVIAEAMHAKGLKASDLAAVGITNQRETTLVWNRKTGQPVYNAIVW
                     QDTRVSGYVEELSKSGGQDRLRAKTGLPLATYFSGMKIRWILENVPGVRALAESGEVI
                     FGNIDTFLLWNLTGGTNGGIHVTDVTNASRTQLMNLATLAWDEEILSLFNIPVAMLPR
                     IVSSSEVYGHISDGPLSGVPLAGDLGDQQSALVGQTCFEPGQAKNTYGTGCFLLLNTN
                     RKPVQSKFGLLTTVAYQFGTQPAHYALEGSVAITGALVQWLRDNLKLISSSAEIETLA
                     RQVDDNGGVYFVPAFSGLYAPYWKSSARGVICGLTRYANRAHIARAVLEATAFQTREV
                     VEAMEKDSGIALDVLRTDGGMIENDLLMQFQADILDRPVVRPAIKETTALGAVYAAGL
                     AVGFFLDLNDLRAQWSVDKTWNPCLSAEKRDHMYALWKKAVTRSFDWVEEPGSVK"
     gene            complement(16052..17707)
                     /locus_tag="VFC15_00420"
     CDS             complement(16052..17707)
                     /locus_tag="VFC15_00420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZM08658.1"
                     /translation="MRHRSEAWRKIDGASFDVCVIGGGATGAGCALDAQLRGLSTVLI
                     EAGDFASATSSRSTKLIHGGVRYLQEAVTDLDPGQYNVVKRALRERRFMLDNARFLAH
                     PMEFLVPCYSRWDVFYYGVGVKIYDRIAGKHNFFPSRYRSSKDSLARWPLLQGDGLKG
                     TIAYADGQFDDARYCVALVQTFTQAGGEAINHARVVRLQQDAGEKICAAEVEDQLTGA
                     NSTMQARIFVNATGPYSDHIRAMANPDLEKRLRLSKGVHILLPLDDRTTDAVVVPKTD
                     DGRVIFAIPWLGRLLVGTTDDEVTHEEELVVKRSEAEYLLRQLNRYLSRPFAVEDIVG
                     GFAGVRPLVSSGKPGRTKKLIREHEVEVDRESGLVSVMGGKWTTYRAMAEDAINAVQQ
                     QLGLPVGGCVTREHHLSGSDGYSADYPQTLAATYGVPDSTARHLSEKFGTNASAVLDL
                     ARQQPELAQPIVAGFPAIQAEIAYSARNEMAATLDDVLERRTGVQFFSWKTAMAAAPI
                     AAAVMQKEMGWDSAHTQREVDDYVHTISEWTQKIGMANTDAKA"
     gene            complement(17704..18258)
                     /locus_tag="VFC15_00425"
     CDS             complement(17704..18258)
                     /locus_tag="VFC15_00425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZM08659.1"
                     /translation="MTIRQLSSRVVYRNTWMTVREDEIERDNGARGIYGVVEKYDSAI
                     ILPIDDDHIYLVEQYRYPIGEKSLEFPQGSLEKNGIDPAEIARDELQAETGLVAANLE
                     YLGEIYIACGYANQKTHAFLATGLTQTARKPDVEEHDLAVRKVSLAKLQQLIRGNVIK
                     DAQTLAAWAFYEARAASRGSVSLT"
     gene            18336..19520
                     /gene="solA"
                     /locus_tag="VFC15_00430"
     CDS             18336..19520
                     /gene="solA"
                     /locus_tag="VFC15_00430"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZM08660.1"
                     /translation="MAKVADIAVVGLGAVGSATLYQAAKLGAKVIGIDRFQPPHDQGS
                     SHGETRITRQAIGEGREFVPLVLRSNEIWEELEDATGRSLMTRCGGLVLAAPDVGGSH
                     HGSSSFLLDTVNAAKEFGIPHQQLGGDDICRLYRQFRLADGEQGYFEPGAGFLRPEAC
                     VETQLEEAKKLGAKVFTSETVLKVRAAGDAVEVKTDKASYSAAKVIVTAGPWIAELLP
                     PEYSSHFRVYRQTLCWFALRQNFERYSPERFPVFIWITGSRVQDMLYGFPAIDGPHGG
                     LKVATERYENTVDPDAVSRDVSDESTAAMYSEYIAQRLPDVSPKCLRTATCLYTVTPD
                     AKFVIDCADASQNILFASACSGHGFKHSPAVGEALAQRAVGLPSRINLSSFALERLRQ
                     QR"
     gene            complement(19524..20354)
                     /locus_tag="VFC15_00435"
     CDS             complement(19524..20354)
                     /locus_tag="VFC15_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08661.1"
                     /translation="MRFCRHMMFATMLFWALSGIAQAPEAQPSAPEAPAAQAPAPQTP
                     AAAAPVSETPQPQAATEPPQKTAAEILAEEKKQRMLGIVPMYGVTNYQYAPALTKGQK
                     FDLMIRAYASPFPYLSAGFQAGLSQAFNSFEGYGQGAAGYGKRFGSAFVDGFDSGFFS
                     NYFYPVLFKQDPRYFRLGNSSTKKRRMASVIEQEFVTKKDSDRKPVFSYSNVLGALTA
                     GSISNAYYPQEERGFGLTMSRTAVSFGWGMVGNFVLEFWPDISHKLRHKSTPDDVTVK
                     "
     gene            complement(20523..>21205)
                     /locus_tag="VFC15_00440"
     CDS             complement(20523..>21205)
                     /locus_tag="VFC15_00440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZM08662.1"
                     /translation="DIVGVVHGERVGGDFYQFARATPTRVLFGLLDVAGAHKENHGIV
                     EAANQTFQEKGPKCLASEELNEADAMMEFCHELNVTIVRAASGVRTCAAFVGCYNEDL
                     GTFSYVNAGHTPGLLRYGSDVSELAATGMPFGLFVGSTYEAPIAAVPRGGALLLVSRG
                     VVEATHKKEEFGLERLKQQLEQTPLTNAHDVASAALQAVRQYAHSAATKNDLTTLALV
                     RPRPSKGA"
BASE COUNT         4217 a         6287 c         6306 g         4395 t
ORIGIN      
        1 cctccctcat ccgctcatag ctcatcgcct tgtgcttgct ggcattgccc tgcatcttgc
       61 tgccatcgat cgccacgtgt cccagcttca ccagtcccgc tttctcaccc aactgcaagg
      121 cttggcaaaa cagccccgcc agcgccttca gatgccgttt gcgaaactcc gccaaagtgc
      181 tatggtcggg atgttcgtcg gccgccaagt accgaaaccc tatatcttcg taggtctttg
      241 ccgcgatctt gcgcgagctg tagctcccca cgcaatagcc gtacagcagc acccgcacca
      301 tcatcctcgg ctcataggcg gcttgcccgc gcccatcctt ttcctcatag gactcgtaga
      361 tggccccaag atccagggca tccaccacct ccaccagaaa gcgtgctaaa tgcttttctg
      421 gcagccaatc atgcagcgaa ggcggcagca gcagcgcctg gttcacatcc gcggagcgaa
      481 agcactttcc catggccgca tcttaacccc atacgcccgt tggaaaagtt attatgtttg
      541 gtgcttctgc agaatctatt cccgccagac tcctagaggc tctaacacat ctcagcttgc
      601 gccacagcca gagccgcgat ggccgccgtt tcagctcgca ggatgttgtc gcccagggaa
      661 gccgcgatcc atcccgcagt ctcgaagagt tgcaattcct caggcatcca tccgccctcg
      721 ggaccaatgg ccatcgccag cgacttcagt tccggtcggc cgcggagaat ttcgctcaac
      781 tgcgcttcct tctcggcctc cgccaagacc actcgtagct ccgcaggagt ctcaagagcg
      841 gtgggcaatt tcaccggatc gccgatctca gggggcgcga cgcgacgcga ctgctcggag
      901 gcttcacgag caatgcgacg ccaacgttcc acgcgcttat cagcagcgag ggcgagatgc
      961 ttctcggtgc ggcgggcgat caccggcaca atggacgtca cgttgagttc agtgcacttc
     1021 tcgatggccc actccatgcg gtcgaactta aacaccgcca gcagcagggt aatcggagcc
     1081 gccacgctgg cggcaatctc ttcgccgagg gcgaattcca cgcgctcatc gctgacgcta
     1141 gccactgtgg cgtgacgcac ctggccgtcg cagacaactt cgaactcctg acccacgcgc
     1201 gctcgtaagg tgcgcgacaa atgcgcggcg tgctcgccgg tcagcgcagc ccggtcgtga
     1261 gttacttcgt cggcaatcca gcggcggcgg gtcatcgggt gatcagcaac aatgcgacgg
     1321 caattgcaat ggcagcatac acgccgccaa aatagcgatt ctcgcgggtg agtgcttctt
     1381 cgtcgaagat gcggctgttc atcaggaatt cgagggccga cacagctccg accagcagcg
     1441 caatcaccag ccagtgttgc tgttgcatgg gggagcgcag caggaatggc gtcgccagca
     1501 atgctaagcc aagatagatg gcgcccccaa tgcgggcccg cttgcggcgc gtgggattct
     1561 gataccgcag cagcaagatg cgcgccaggt cacgcggacg tgccaggcgc gcgaaggccg
     1621 gcagcgcagt cacgatcagt agaaaccacg cgtagatgcg aagcgttttg tccaatgagg
     1681 ttcgtccgca gtgcggttga gtgcgacgat cgatcctagt tgaaaatgtc tcgcatcttg
     1741 cccaacaacg tacggctcac gggcttgttc tctacgctga gcgtgctctg caactcctgc
     1801 aacaattcgc gctggcgttt gctgagtttg gcaggcgtct gcaccctcaa ctcgacgtac
     1861 agatcgccgc gtccccgtcc attcaattcc ggtacacctt tgttcctgag cttgaaggtc
     1921 gtacccgtct gcgttccctc cgggactttc aacgtgtgct caccttccag tgtcgggacc
     1981 gcgatctcgg tgcccagcgc cgcctgcacc acagaaaccg ggaccacgca gcgcaagtct
     2041 ttgccttcgc gctcgaagaa ctcgtgctcc ttgcaatgca acaccacgta caggtcgccg
     2101 ggaggtccgt tgtagattcc cgcttcgcct tcgcccgagt acagcatgcg cgcgccttct
     2161 tcgacaccgg cgggaacctt caccgtcacg ttgcgctcgc ggcgcaggcg ttgctggccc
     2221 ttgcacttgg gacagggatc gataatcagc ttgcctgagc cgtggcaggt gccgcagggg
     2281 cgcgcaatgg cgaagaagcc ctgctgatag cgaacctgtc cgcggccgcc gcaggtggtg
     2341 cagtcgatcg gcgctttgcc gggagctgcg ccgctgcctt tacactggtc acattcttcc
     2401 aggcgccgga tttgcacttg cttggtgacg ccgaagaccg cttcctcgaa ttccagcgtg
     2461 aggtcttcgc gcatgtccgc gccgcgctgg gcgcgcgagc ggcgtccacc acgcccgccg
     2521 ccaaatatat cgccgaaacc aaaaatatcg ccgaagatat cttgaatgtc agggaagccc
     2581 gatgggtcga agccgcctcc gccattgccc gagactccag cgtggccgaa gcggtcatag
     2641 cgagcgcgct tctccgcgtc agccaacacg gcgtaggctt cgctgcattc cttgaactgc
     2701 tcttccgctt ccggattatc gggattgcgg tcaggatggc actgcatcgc ctgcttgcgg
     2761 taggcactct tcagctcctg gtcggtggcg gtgcggccca cgcccagaat ttcgtagtag
     2821 tcgcgttttt ctttggtagc cagcgttgtt tcccttgcaa tcttagatta cgaacctagt
     2881 gtttaggatt gtgcgccacc cgcaccatcg cgggccgcag cagccggtct ttgatgcggt
     2941 agccgcgctg caactcatcg atcacatggt tgtccgccac ttccgctgta tcaaccatct
     3001 gcaccgcctg gtgcaggttg ggatcgaagg gttcgccctc cgctggaatt ggttccacgc
     3061 ccaacctcga cagcgcatcg tgaaattgct tgtcgatcag ctcgatgccg gatagatact
     3121 cctgcaccga tgtggcgttg gtcttcaacg cgcgatcgag gctgtcgaga atgggcaaca
     3181 gcgacttgag cgcctcggcc agagcatagt cgcggaagtc ctgttgctct ttcacgctgc
     3241 gtttgcggaa attgtcgaac tcggcctgca gccgcgccag gcgatcgaga tagcccgcgc
     3301 gctcttcacg caacgtctcc aattcagctt cgggatcagc cgctgtcgca ccgcctccgc
     3361 gactgcgcga aggaatggca tcctcgctct cagcggatgg cagttcgtgc tcgaggtcca
     3421 gttcgctggc ctctccgttc tttttattca ttgccataat cgtctctagg tcgattcatt
     3481 ccagaattgg tcaaacatgc gcgcaatgta ggaaaccgca gtgatggtgt gctcgtagtc
     3541 gagccgtgtc gggccaatca ccgccagcga accgactacc tcgtccccga cccgcgccgg
     3601 tgtgccaatc agtacgaagt tttttagatg cgggacagcg tcttcaagac ccacaaccac
     3661 gcggactgcc tcctggcgcg cgtccaagta cgccgagagc aggtcgacaa tgcgctgccg
     3721 ctcttccaga gtggccagaa gttgtcggag gcgttcccgg tcttgttcgt tgagcaccag
     3781 gttcgatgcg ccttctacaa acaccgtctg ctgagccgct tcggtcgcga gcgctccctg
     3841 ggtatagagc tgctccaccg agttcatcag ccggtcgtac tcggtgcgtt cctgctgcac
     3901 gcgcttggca atctcgttgc gcaccgcctc catctgccag ccacggaagt tctggttgat
     3961 gtagctggcg gcgatctcga ggtcgctagc ttggaggtcg tcagtcaagc gcaacacgcg
     4021 gtcgcgcacc aacccgttgc gcatgacgac cactgccagc actttcttgt caccctggcg
     4081 ctggaaatac acgtgctcaa gttcgttgcg ctgtccgtga gctgacgtga cagtcactcc
     4141 tacgccatgc gaaatcagcg acagcacgtg cgaggtgcgc tccatgaatt cctgtacgtc
     4201 gctcgatccc tgcaggttgc cctgaatcag gctctcgtca gcaggagaca gacgcgcttc
     4261 gccagtcagt tgcttgacgt agaaacgata ggcttgcggc gtggggatgc gtccggcaga
     4321 ggcgtgtggc tgctcaagga atcccgcgtc ggccaaatcc gacatcacat tgcggatgga
     4381 agccgggctg aggctctcgc ggttgctgcg ggcgatggtg cgcgatccca caggctcgcc
     4441 cgtcgagatg taggtctcga cgatggcggt aaggatttcg cgttcgcgct tgccaattcc
     4501 cggaggtgag cccatgttgc ctcggtttga ttaacccatt atttgtcaat tagttacata
     4561 atcaatagag aaacacaact gcaatctaat tctaggtagc agcgggcgcg ctgtcaagat
     4621 tggcactcag ccaatatgag tgccaataac tcaccacaca tctagaaggt gtgaccctga
     4681 taccggtttc actgctggtg ctgctcgtgc ctgatggtgg tgatatagtc gactatatca
     4741 ccaaaaaatt actttcatgc tgttgtttag aatcaaaggt ttgggcggaa attgttcgca
     4801 agtcattgat tgtgaattac ttatatctat ccacatctca gcaaaggact tagcagcctg
     4861 gttttgagtt cgaaggtccc gttcccggtc atccttcggc gcttgtcagc gattctggtt
     4921 gtcaaagagc gctatccact gcgcattcag cgctgaacgg cgaggcgaag aaatccctaa
     4981 cctcacagat ctggtatagc gcggcaggct cccgatgaaa agaagaatcg acaggtggag
     5041 gaacgaaagt aaccaccgtg cttttcccca agcctcactc ccgaagccct gtccgtgaag
     5101 atccgtgcaa atccgcggtg cctgtgtctt ttcggtactg tcctaatctg ctctggcatg
     5161 tcgccggggt tggagagata actgccttca tgatagccga agccaagagg ttgacgttct
     5221 tttgcatcga gtgcgacacg aaactggaag agccagaaac gtatcaatat ggcggtgagg
     5281 tcgcgtttgc gtgcgagaaa tgcgtaagga atcattacga agaactttat cgacacaacc
     5341 catctgagat gcaagaggaa ctgcgtggta ggcgtttcag tgccgcagca tcaataaagc
     5401 gcaatcggcg taaattggaa aagcaggcgg caaagagagc ctaatgccac gactcacgaa
     5461 atgcgtaatc agcgttaagg ctgccttggc tcttagaaag cacagccttg cactagacga
     5521 gttccattgt ccgaaatgtg gggaattggt tgacgcacac aaagcaggag aaggaccgac
     5581 cggcatcaaa agcgcagccc actttgagca ccgcgttggc aacgcggcat gtccgtacag
     5641 ccatagccat cggaaagccg caataattta ggacagcacc gccttttcct agttcttctc
     5701 cgcgtctccg tgcctcggtg gtgaaagctc tcactgtccc agcaaatact tctgcgtgaa
     5761 caacactgtc gtatagaact ggaagtcggc gttctttttc ttggcgaagc cgtggccttc
     5821 gtctttcgcg gcgagatacc acaccgtcgt cccgttcgtc tccagcgctt tgaccatctg
     5881 gtcggcttcg ctcttgggca cgcgcggatc gttgacgcca gcgacgacca tcatgggctt
     5941 gttgattgcc ttgacgtgat tcataggcgc gatgctttcc aggtaggcgc gcatcttggg
     6001 atcgcgttcg tcgccatact caacgcggcg cagatcgcgg cggtaggcct cggtgtgctc
     6061 caggaacgtg accaggttgg agatgccgac gatgtccacc gaacaggcaa tgcggtcgtt
     6121 gtagagggtg gcaacggcga gcgtcatgtg tccgccgtag ctgccgccgg tgaccagcac
     6181 tttcttcgca tcgaggtcag gctgggtggc gatccagtcg agcagcgcgc cgatatcttt
     6241 gtaggtgttc tcgcgcagga atccgttatc gagttgcgtg aacgccttgc cgtagcccgt
     6301 ggagccgcgc acgttgggga agatcaacgc cacgcccatt tcgttgggaa gatagttggt
     6361 gcgtccgagg aaggtcggcc gcgactggcc ttcggggccg ccgtgaatga cgatctcgac
     6421 cggatacttc cctgccgcgg gcttgtcttt cggcagatag agccagccgg aaatttcttt
     6481 cccatcaaag ctcttccaag tgatgagctt gggttcgacc aggttctgcg gctggatgcc
     6541 gcctgtttcg ctcatggtcc agcgatcgag cttcgccgtc tggatatcaa ctgagtacac
     6601 gtccgaggga gaccgcgatg tattcagact gaacgcgagg tctcgattgt cttcgtgcca
     6661 gcgcaagttg gtgatcacgc ccgctggcag cttagggata cgcaactctt tgcctgaatc
     6721 cacgtcaagc acgtgcagtg tgctcatacc gttttcgttg agcacgaagg cgatatgacg
     6781 gtgatcttcg gagagttgcg caccgtcctc gatgtcccac ttataagtgg tgagatactt
     6841 cagcgacttg gtcgccaggt ccatgtaggc gaggcgctgg tactcgttgt ccttgtcggt
     6901 ggtgaggtag atgcccttgc cgtcgcggct gaatccgatg ggggtatagg aaactttgtc
     6961 gctgcccttg ggagtgagtt cggtcttctg tccggaggcg gcatcgacca gccaaaggta
     7021 actctcgttg atcgaaactc cgttgaacac gagcagcgtg cggtcgtcgg gtgaccagtc
     7081 gctcacctgc catccgccac cctggttctg cgcgatcatc ttgtcgctgt ctttcttagt
     7141 cggatccatc acgaagaggt cgaggtcggc gttgttgcga cgggtgctgg cgtaggcgat
     7201 gtggtctccc ttgttcgacc aggcgaattc ggagttgcgc gacttgccgt cggtgagcaa
     7261 cgtcgattcg ccagtggcga cgtcgaagcg gaaaatctga aaccactcgc cgccgcctac
     7321 gtccttgcgg aacaaaaagt agtcggcgga gtgcggatga tagctggcgt ctgcgatgcg
     7381 atcgggaaag aaggtaagtt gggtgcgagc ccctcccggc atggcgacct ggtgcacctg
     7441 gttggtgtca ccaaagcggg tcccgatcag gatttcgcgg cgttgcggat gccagcccca
     7501 catagtcgcg gagcgaaact gggtgtagcg gtcagcgcgc gcggcgatgc cggcgggaat
     7561 cggcgggatg ttctccagga tgagcgcatc gttgggcgta atggcgacgg gcgcgatggt
     7621 ggcttcctgg gcgaggcccg tcgcgcagag tccgagaaaa atcgtgccaa tcagcaactt
     7681 acgtaatgtc ataagcaacc gcttcctttt cagtcggatg tgagaggacc ggactgattg
     7741 aacatcaaac gacaagggta tgagccacca tgcccccctg taaagtgata cagttgttac
     7801 agatgtaagt ggcaagcttg gtgtttggga agacgagtga atcgttttcg ttcctgccga
     7861 gtctaattag gggtgagtga aaggagcgtg gtggatggca ggaagttctc gtacaacctt
     7921 cacaaagcgg cagaaagaac gtgcccgcca ggaacggcag gcggaaaagg cccagaaaaa
     7981 ggcccaacgc aagcaggaac cgaattcccc caccgaagct cccgcagaac tcgtgatcac
     8041 acatgatgaa gaaggtcagc cacttggctt tgacttccac gatttcggca agtagttgct
     8101 tacataggtt ccctttcggc gcgatccgcg gtatcgctga tggcgctgtg gtatagtcca
     8161 ggtagacgct tattgccacg gtagatgtgg tgcgaaaagt ccgatcctag tttgatctga
     8221 ttcccgcctc tcgactcccg taaaactttt agcgatcgac caaactcgag cggcatcagc
     8281 tgtatccttc gcgactcgca tctcagtatg catatgcgcg agttgcccct cctagaaagt
     8341 aaaaaacgat tgacacaatt tagcgatatg tccctgtctg ctgggctcca gcagaagttg
     8401 gcggcagcac actttacaac acccactccg gtccaggaaa tggccatccc gcatgcgctg
     8461 gaaggcaaag acgttctggc gacagcccag acaggcaccg ggaaaacttt ggcgttcctg
     8521 cttcccatca tggagaagct gggagcgaaa catttgccag gcgtgcgggc actgattctt
     8581 gttcccacgc gcgagctggc catacaggtc caggcggttt atgacaagct gcgtttcggc
     8641 aaactgccga agaccgcgct ggtgatcggc ggaatggccg agcgtccgca gatcgaagct
     8701 gtgcgcaaag gcgcaggcgt catcgtcgcc actccgggac ggctggaaga ctacctcaag
     8761 cgccggctgg ttgacctgag caaggtcgag actctggtgc tcgacgaatc tgaccgcatg
     8821 ttggacatgg gcttcttgcc ggcattgcgc cgcattgttg ggtcactgcc taagcagcgg
     8881 cagacgctgt tcttctcggc aacgctggaa gcctcggttg caggactggt caacgattac
     8941 atgcgcaacc ccgtgcgggt cgcgctgggt tcgatcagca agcccgtaga gagcgtcgag
     9001 ctgcacgctt tcgaagtacc ggcggcagac aagtccgaca cgctgcgccg cttgctttac
     9061 gacgagcagg gtcagaccct gatcttcgcg cgcaccaagc gtggcactga gcggctggcg
     9121 aagaacttga ttcgcgatgg attctcggcg gcgatgatcc acggcgaccg tacgcagtcg
     9181 cagcgcgtca acgctctgaa cggcttcggc gccggcacct acaaagtgct ggtggcgacc
     9241 gatgttgctt cgcgcggcat ccacgtggat aacgtggcgc acgtgattaa ctacgacctg
     9301 cccgatctgg cggaagactt catccaccgc gttggccgta ctggccgcgc cggattgaag
     9361 ggacgcgctt ccaccctggt ttcaggggcg gagatcggcg aattgcgggc catcgaacgc
     9421 accttgaagt tgaagatcgc gcgcaagcgg gtcgacggct ccgagattgc ggcgatgaag
     9481 ccccgcatcg tgaagaacac tttggactcg cgcacactgg cacagttgcc gggcgaagtg
     9541 ttctgctaag ttttgttccc gccctttcgc aagaaacaat gcgaaaggat gggacatcaa
     9601 gttctgatta ggtttcgtat cttcgccaac cagcgagtcg atgcggaacc ccgaaaagat
     9661 tcttcccatc ctgagcgccg aaactgacgc aacggatggg agtaggtcgc caagtttcgt
     9721 caaccaacga aagaacgggc cgccaaaact tagtttgcaa cgaaaagccc cgggaactcc
     9781 ggggctttcg tgcgtctgaa acttgaggtc ggactacctt tgtttttcat cgaacccgta
     9841 cttgagctgg cgctgatact cggcctggtc aaagtgccct tgtgactccg cgatcaagct
     9901 atccgcgccg aacgtccact tctcgaatcc gatgatgcga acgcgcttgc cggtcccgcc
     9961 cggcccggtg tttgtgccgt cgagcatcca tcggtaaaag acatggccgt tggcagtggt
    10021 gatgtcgagc gccgtcaaca gcatgtcggg aaatgcggtc atgaatccgt gagccacctc
    10081 ggcgatcgca tcccgcccca ctgccggaac accattcacg ctgagcgatc cgttcggcgc
    10141 gaaatatgcg gctatgctcg caggatcttg actgcaccac gccgcggtat agcgctcggc
    10201 aaaggcgcgt agttgagacg gggcaagtgt catcgcctac tgaatctcga tcaccggccc
    10261 ggatgtggtc ttgacctcgc tgacgcggtc gaccacgtgg cgggcgaatt tgaacagcga
    10321 gcgcgcgtgg ggcgactgat cgccttccag caccacgggc tgtccggtat cgcctccctt
    10381 gcgaatgtcg ggatcgattt cgatgctgcc gagaaagggc acgccgaact gctgcgccgt
    10441 gcgctcgccg cctcccttgg agaaaatgtc gatttcgtgc tgacaatgcg ggcacaggaa
    10501 agcgcccatg ttctcaacga cgccgagaat gtcgaccttc acctgcttga acatctcgat
    10561 ggccttgcgc gcgtcctgca gagcaacatc actgggcgtg gtgaccacta cggcaccggt
    10621 taccggcacg gtctgcacca gcgaaattac aacgtcgccg gtaccgggcg gcagatcgat
    10681 gatgaggtag tcgagctgtc cccactccac ctggccgaga aactgcttca tgatggaatg
    10741 cagcatgggc ccgcgccata ccagcggctt gtcgcccggg ctgagaaaac cgaccgagat
    10801 taccttcagt ccataacgct ccggcggcag aatacggtca cccttgacga cttcgggcgc
    10861 aactgtcgtg cccagcatcg taggaacgtt ggggccatag acgtcggcat cgagcagccc
    10921 gactttgaag cccatgcggg cgagcgcgat ggagaggttg acggccaacg tggttttgcc
    10981 gacgccgcct ttgcccgaac cgacggcgat gattgcgtcc acgccgggaa ggggttgcgg
    11041 accggccggt tcggggccgt gcgaatgtgt atgagaactc aagggtggtt acctgctttg
    11101 ttcaagatgg gatacgcaaa gggtgattat acgacgcgcg ggcactactc ccgctcatag
    11161 cggatcttga atggacatag ctccctcgat ggaagacggt gcggcagtgt gctgcgttgt
    11221 tgcgaccaac tgacagtccg atgggatgaa acgctggtca gcgttgccgc tctactgtta
    11281 ccatcgaggg cgtaaatgca tccggtttcc tacaacccgt gttcgcagtt ccggatcgag
    11341 attgctccct gcaatttcca ttgtcgttgt tgtcgctaga cgccctgttc cctcgtagct
    11401 gtgaaatctg aaaccaaaag ccttcaggtt gagtcctaac tgcatggaga gcgttcatga
    11461 agatcgccaa tgacgtaact gaactcatcg gcaacacgcc gctggtacgc ctgaatcggg
    11521 tgaccaaggg cgtcggtgcc ctgattgtcg ccaagctcga gagcttcaat ccctgcgcca
    11581 gcgtgaagga ccgcatcggc gtcagcatga tcagcgaggc agaaaaggcg ggaaagatcc
    11641 atcccggcag gaccacgcta attgagccta ccagcggtaa tacgggcatc gggctggcgt
    11701 tcgtcgcggc ggtgaaggga tacaagctga tcatcgtgat gcccgatacc atgagcaccg
    11761 agcgccgagt gctgctgcgc gccttcggag cggaactggt gttgacgccc gggcccaagg
    11821 ggatgaaggg cgcaatctcg aaggcgcagg aaattctcgc caagacgccc gatggcttca
    11881 ttccgcagca gttcgacaat cccgccaatc ccaagatcca cttcgagact accggcccgg
    11941 agatatggaa cgacaccgac ggcaaggtcg acatcctgat ctctggggtc ggcaccggcg
    12001 gcaccatcac cggagtgtcg gagtacatca agccgcgtaa gccttcgttc aaagcgattg
    12061 ccgtcgagcc tgtcgaaagc cctgtgctct ctggcggcca accttcgccg cacaagattc
    12121 aaggcatcgg cgcgggtttt gtgcccagca ttttgcgcac cgattacatc gacgaaatcg
    12181 tgcaggtttc gagcgatgat gcgctggtaa tggcacgtcg ccttgcggtt gaagaagggc
    12241 tgctaatggg catctcatca ggggcagcgg tggtggcggc cattcgcgtc ggcgagcgcc
    12301 cggagaatgc gggcaagatg attgttgtgg tgctgccgtc gtttggcgag cgctacctga
    12361 gcagcattct cttccagcaa ttgcgcgacg aagtagtggc ccagccgacc gagccggtat
    12421 cggtgtagcg tcggagcaaa tatggtttcg tacttccggt taatgcgtga agacatcgcc
    12481 gcggtgctcg aacgcgatcc cgcggcgcgg tcgaagctgg agatcgtgtt gctgtactcc
    12541 ggcctgcacg cgctgtggtg gtaccgcatt caccattggc tctggaagca tggctggctc
    12601 ttcctggccc gggcgctttc acaattcgct cgttggctta ccggaattga aatccatccc
    12661 ggcgcgaaaa tcggccgccg cttctttatc gatcacggca tgggagtagt gatcggtgag
    12721 accgccgtgg tgggcgatga cgtcaccatc tatcaggggg tcacgctggg tggaacttcg
    12781 ctgaaccatg gaaaacgtca ccccaccatc ggcgatcggg tggtcatcgg tgggggtgcc
    12841 aagattctgg gcaacatcac cgtcggcgag aactgccgca tcggcgcagg atcggtggtg
    12901 ttgcgtgacg ttcccgcgga ttcaaccgtg gtcggcgtgc ccggacacat cgtctttcgc
    12961 cagggcaagc gcgtggtcat caccgatccc aagcagatca acgatccatt gtcggaagcc
    13021 ctggccgcag tcgcgaacga ggtgaagacc ctgcgtgacc aggtttgccg cttggaaggc
    13081 cgagagcacg tgccggccga aggcggcgat tcgctcgacc tagcgataga gatggattac
    13141 cagatttaga ggaagtagct tgcgctggac gcgacactag tagattccaa gtagtgcgtg
    13201 ctcagagttg gcccggtaga atcacgcggt gcgtatcgaa ctgctgcatg cgaaactccg
    13261 accctccagt ctcggcggag tcgctgcagg cttaacgata tatgcgcccg ccgcgtgttt
    13321 attctgcctt tactttctgc tgctgccttt ggccatgaac gctggcacca tcgagcaccc
    13381 cggcattctt cacaaggaag acaactgttc ctcctgtcac gtggataaga cccgaggcaa
    13441 gtcggtgcac tctgccatgg aggttccgtg cacggtctgc catctggccg aaacccaagg
    13501 cgacatgact accctgcgac tgtcccttcc gaaggcggag atctgcttct cctgccacga
    13561 gaagaccatg ctgttgcggc aacacataga aggcgggaag aaactatgcg tggactgtca
    13621 cgatgctcac agcagcaatc gacgtatgtt gctactggat ccactcaacg caaaccgccg
    13681 caactcttcg gtcatgctga agtagggccg caacgggaca ttacacgatg tgcgccgcct
    13741 tgacgatcac cgccgctagc gccgctccca gcagcgggcc aaggatcgga atcggcgcgt
    13801 aggcccagtc cgaggttccc ttgcctgcca ctggccacag cgcgtgcgcc acgcgcggac
    13861 caaaatcgcg cgcgggattg atggcgtaac cggtggttcc tcccagcgac aatccaatcg
    13921 cccacaccag cgcggagacg aagtaggggc cgaggccggg cgtcagtccg gtgggagaca
    13981 cggccttcga gccgatggcg accacgacga tgattaacag catcgtgccg atcacttcgc
    14041 agaagatgtt gccgggataa tgacggatcg cgggcgcggt acaaaagacg gccagcttag
    14101 cgtcgcggtc ttcggtcatc ttccagtgtg gcaggaaata cagccagact aaggtcgctc
    14161 ccgtgaacgc gcccaaaaac tgcgcgctcc aatagggaac gattttcgcg aagctgccgg
    14221 aggtgatggc aaaggccagc gtcaccgcgg gattgatgtg cgcgtcggcg ctgccgaatg
    14281 cgacggcagt gaaaatgccg cacaacacgg cgaacgccca gccggtggtg atgaccatcc
    14341 atcccgcgcc ctcggccttg gagcgcttca gcaacacgtt ggctaccacg ccatcgccca
    14401 gcaggatcag caccagggtc cccgcaaact cgcccatgaa gggtgaagtc atatgatttc
    14461 gatctcctga caactgttgt ctgaactatt tgacgctgcc cggctcctcg acccaatcga
    14521 agctgcgagt gaccgccttc ttccacagag cgtacatgtg gtcgcgtttt tccgccgaca
    14581 ggcaggggtt ccaggtcttg tccaccgacc attgcgcgcg caagtcgttg aggtcgagga
    14641 agaatcctac cgccagccca gccgcgtaaa cggcgcccag cgcagtcgtt tccttgatcg
    14701 cggggcgcac cactggacga tcgagaatgt cagcctgaaa ctgcatcagc agatcgttct
    14761 cgatcattcc gccatcggtg cgcaacacgt cgagcgcaat gccggaatcc ttctccatcg
    14821 cctccaccac ttcgcgcgtc tggaaggccg tcgcctccag cacggctcgc gcgatgtgcg
    14881 ctctgttcgc atagcgcgtc agcccacaga tcacgccccg cgcgctggac ttccagtagg
    14941 gcgcgtaaag cccggagaag gcgggcacga aatagacgcc gccgttgtcg tccacctggc
    15001 gcgccagcgt ctcaatctcg gcgctgctgc tgatcagctt caggttgtcg cgcagccact
    15061 gcaccagggc gccagtgatg gcgacgctgc cctccagcgc gtaatgcgcg ggctgtgtgc
    15121 cgaattgata cgccacggtg gtgagcaatc caaacttcga ctgcacgggc ttgcgattcg
    15181 tgttcagcag caggaagcat ccggtgccgt aagtgttctt cgcctgtccc ggctcaaagc
    15241 aggtctgtcc taccagcgcg gattgctgat cgcccaaatc tcccgccagc ggaacgccgc
    15301 tcaacggacc atccgagata tgtccgtaca cctcgctgct ggagacgatg cgcggcagca
    15361 tggcaaccgg aatgttgaac aggctgagta tctcctcgtc ccacgccaga gttgcgagat
    15421 tcattaactg ggtgcggctg gcattggtca catcggtgac gtggatgccg ccgttcgtcc
    15481 cgccggtcag gttccatagg agaaacgtgt cgatgttgcc aaagatgacc tcgccggatt
    15541 cagcaagcgc gcgcacgccg ggaacgttct ccaggatcca gcgaatcttc atcccgctga
    15601 aatatgttgc cagcggcaga ccggtcttag cgcgcaggcg gtcttgcccg ccggactttg
    15661 acaactcttc aacgtagccg ctgacgcgcg tgtcctgcca gacgatggcg ttgtaaaccg
    15721 gctgtcccgt cttgcggttc cataccagcg tggtctcacg ctgattggtg atgcccacgg
    15781 cggcgaggtc gctggccttc agacccttcg cgtgcatcgc ctcggcgatg acttcctgcg
    15841 tgcgcgtcca gatttcctgt ggattgtgct ccacccatcc tggccgggga tagagttgct
    15901 catgctcttc ctgcgcggtc gcgacgacct gcccagagcg gctgaagacg atgaagcggg
    15961 tgctggttgt gccctgatcg atggcgccga cgtaatccgg catttcgctg tccttgttag
    16021 tgcggatgaa attcagtcca agagaaaggt tctacgcctt cgcgtcggta ttcgccatac
    16081 caatcttctg cgtccattcc gagatggtgt ggacatagtc gtcaacttcg cgttgggtgt
    16141 gagccgagtc ccatcccatt tccttttgca tcaccgcggc ggcgatggga gcggccgcca
    16201 tggcggtctt ccagctaaag aattgcacgc cggtgcggcg ttccagaaca tcgtcgagag
    16261 tggcggccat ctcgttgcga gcgctgtagg caatctccgc ctggattgcg gggaatcctg
    16321 cgacaatcgg ctgcgccaac tctggttgtt gtcgcgccag atcgagcacc gccgacgcgt
    16381 tcgtcccgaa cttctcggac aggtgacgcg cggttgaatc cggcacgcca taagtggcgg
    16441 ccagggtctg gggataatcg gcgctatagc catcggaacc gctgagatgg tgctcgcggg
    16501 tgacgcaccc gcccaccggc agaccaagtt gctgctgcac cgcattgatc gcgtcttcgg
    16561 ccatggcgcg ataggtagtc cacttcccgc ccattacgct gaccaggccg ctctcgcgat
    16621 cgacctcgac ttcgtgttcg cgaatcagtt tcttggtccg gccgggcttg cccgaactca
    16681 ccagcggacg cacgccggca aagcctccaa ctatgtcctc taccgcgaag ggccgggaga
    16741 ggtagcgatt caactgccgc aggagatatt cagcttctga gcgcttcacc accaactctt
    16801 cctcgtgcgt gacttcatcg tcggtggtcc cgaccagcaa gcgccccagc catgggatgg
    16861 cgaagatcac gcgaccgtca tcggttttgg gcacaacgac agcgtcggtg gtgcgatcat
    16921 cgagcggcag caagatatgc acacccttgc tcaggcgcaa ccgtttctcc aaatcgggat
    16981 tcgccatcgc gcgaatgtgg tctgagtagg ggccggtggc gttgacgaag atgcgcgcct
    17041 gcatggtgga attcgcgccc gtgagctggt cctcaacttc ggcggcgcag attttttctc
    17101 cggcatcctg ttgcaggcgg acgacgcggg catgattgat cgcttcgcca ccggcttgag
    17161 tgaaggtctg caccagcgca acgcagtaac gcgcatcgtc gaactgtccg tcggcgtagg
    17221 cgatggtgcc cttcaggccg tcgccctgca aaagcggcca gcgtgccagc gaatccttgg
    17281 acgagcgata ccggctgggg aagaagttgt gcttgccggc aatgcggtcg tagatcttca
    17341 cgccgacgcc gtaatagaag acatcccagc gcgagtagca ggggacgaga aattccatgg
    17401 gatgagccag aaatcgcgcg ttgtcgagca tgaatcgccg ctcacgcagg gcacgcttga
    17461 ccacgttgta ctgcccggga tcgaggtcgg tgaccgcctc ctgcaggtag cgcactccgc
    17521 cgtggatcag cttggtcgaa cggctggagg tggcggaagc gaagtctccg gcttcgatca
    17581 gtacggtgct gaggccgcgt aactgcgcat cgagcgcaca gcccgcgccg gttgcgccgc
    17641 ctccgatgac acagacatca aagctcgcgc cgtcaatttt tcgccaggct tcggagcgat
    17701 gtctcatgtc aagctcactg aaccacgact tgcggcgcgc gcctcgtaga acgcccacgc
    17761 cgccaaggtt tgcgcgtcct tgatgacgtt gccgcgaatg agctgctgca gtttcgcgag
    17821 actcaccttg cggacggcta ggtcgtgctc ctccacgtca ggcttgcggg cagtttgggt
    17881 tagaccggtt gcgagaaacg catgggtctt ttggttggcg tagccgcagg cgatgtagat
    17941 ttcgcccagg tactccagat tggcggccac gagtccagtt tcagcctgca gttcgtcgcg
    18001 cgcgatctcg gcggggtcga tcccgttctt ctccaggctg ccctgcggga actccagcga
    18061 cttctcgccg atgggatagc gatactgctc gacgaggtat atgtgatcgt cgtcgatggg
    18121 aaggatgatg gcgctgtcgt atttttctac gacgccgtag atgccgcgtg cgccattgtc
    18181 gcgctcaatc tcatcttcgc gcacggtcat ccaggtattg cgatacacca ctcgcgagct
    18241 taattggcga atcgtcatgg atggtttccg ggaaacagtc tctcatgcga gggcaaattc
    18301 ccgccgtgat ttgaggcgtg tggcatcatg gcggaatggc gaaggtcgcg gacatagcgg
    18361 tggtcggatt gggagcggtg ggcagcgcca ccttgtacca ggcggcgaag ttgggcgcca
    18421 aagtgatcgg catcgaccgc tttcagccac cgcacgatca ggggtcgtcg cacggcgaga
    18481 cgcgtatcac ccgccaggcg attggcgaag gccgcgagtt cgtgccgctg gtgctgcgct
    18541 ccaatgagat ctgggaggaa ctggaagacg cgacgggccg cagtctgatg acgcgctgcg
    18601 gtggactggt gctagccgcg cccgatgttg gcggcagcca tcacggatcg agttcgttcc
    18661 tactggacac cgtcaacgcg gcgaaggaat ttggcatccc gcatcaacag ctcggcggcg
    18721 atgatatttg ccgcctctac cggcagtttc gactcgccga tggcgagcag ggctacttcg
    18781 agccgggggc gggattcctg cggccggaag cctgtgtgga aactcaactc gaggaagcga
    18841 agaaattggg cgcgaaggtg ttcacttcag aaaccgtgct aaaggttaga gctgcaggcg
    18901 atgcagtcga ggtgaaaacc gacaaggcga gctactccgc agccaaggta attgtcaccg
    18961 ctggtccgtg gatcgccgag ctgctgccac ctgaatactc gagccacttt cgggtctatc
    19021 gccagacgct gtgctggttc gcgctccggc aaaatttcga gcgctactcc cccgagcgct
    19081 tccccgtctt catctggatc acgggcagcc gggtgcaaga catgctttac ggatttcctg
    19141 cgatcgacgg accgcacggc gggctgaagg tcgcgactga acgctacgaa aacaccgtcg
    19201 atcccgacgc tgtttcacgc gacgtaagcg acgaatcgac cgcggccatg tattccgaat
    19261 acatcgcgca gcgcctgccc gacgtcagtc ccaagtgcct cagaaccgcg acctgcctgt
    19321 acacggtcac accggatgcc aagttcgtca tcgactgcgc tgacgcgagc cagaacatat
    19381 tgttcgcgtc ggcgtgttca gggcacgggt tcaagcattc tcccgcggtg ggcgaggccc
    19441 ttgcgcagcg ggcagtggga cttccctcga gaattaattt gtcgtctttt gctttggaga
    19501 ggctaagaca gcagcgatag tgtttacttc accgtcacgt cgtcaggtgt gcttttgtgg
    19561 cgaagcttgt gcgagatgtc gggccagaac tcgagaacga aattccccac catgccccat
    19621 ccaaatgaca ctgcggtccg gctcatggtc agcccgaacc cccgttcttc ctgcgggtaa
    19681 tacgcgttgg aaatgctccc cgcggtcagc gctcccagca cgttcgaata gctgaaaacc
    19741 ggcttgcgat ccgagtcctt cttggtcacg aattcctgct caatgacgga ggccattcga
    19801 cgtttcttgg tactgctgtt acccaagcgg aagtaacgcg gatcttgttt gaacaaaaca
    19861 gggtagaagt aattgctgaa gaatcccgaa tcgaacccgt caacgaacgc cgagccaaag
    19921 cgtttgccgt agccagcagc tccctggccg tagccctcga aggaattgaa agcctggctg
    19981 agaccggcct gaaatcctgc cgacaagtat gggaacgggc tcgcatacgc gcgaatcatc
    20041 aggtcgaact tctgaccctt ggttaatgcc ggggcgtatt gatagttggt cacgccgtac
    20101 atcggaacga tgccgagcat cctctgcttc ttctcttccg ccaagatttc tgccgcggtc
    20161 ttctgcggtg gttccgtggc ggcttgcggc tgaggagttt ctgacaccgg tgccgccgca
    20221 gccggcgtct ggggcgctgg ggcttgcgcc gcaggagcct ccggcgcgga cggctgggcc
    20281 tccggtgcct gggcgattcc ggaaagtgcc cagaacaaca ttgtggcgaa catcatgtgg
    20341 cggcaaaatc tcatacggga caaatcgctc atcaagtaat tcgtgctcga agcgggctgg
    20401 agttccaata tgacctgaaa atatcccaca atttgagcta tctaaagact accagagggg
    20461 cactgctaca gccgtgccgc aagcgatttt aggcagttaa gaaatgtaac gcggcgatga
    20521 gtttatgcac ccttggaagg tcgcggcctg accagcgcca gggtcgtgag gtcatttttc
    20581 gtggcagccg aatgcgcata ttggcgaaca gcttgcagcg cggcggaagc cacgtcgtga
    20641 gcgttggtta gtggggtttg ctcgagctgc tgtttaagcc gctccagccc gaactcctct
    20701 tttttatggg tcgcctccac cacgcctcgt gataccagca gcaaagcgcc gccccgcgga
    20761 accgccgcta tgggcgcttc gtaggtcgag ccgacaaaca atccgaatgg catgcccgtg
    20821 gccgccaatt cgcttacgtc tgagccgtag cgcagcagtc cgggagtgtg gccggcattg
    20881 acgtaactga aagtgccgag gtcctcgttg tagcagccga caaacgcggc gcaggtcctc
    20941 acaccggaag ccgcccgcac aatggtgaca ttgagttcgt gacagaactc catcatggcg
    21001 tcggcttcat tcaactcttc gctggccaga cactttggtc ccttctcctg gaaggtctgg
    21061 ttggccgcct ccacgatgcc gtggttttcc ttgtgcgctc ccgccacatc cagcagcccg
    21121 aacaggaccc tggtaggcgt ggcacgcgcg aactgataga agtccccgcc aacccgctcg
    21181 ccgtggacca cgccgacaat atcgg
//
LOCUS       DASRKK010000023        21105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_978279, whole genome shotgun sequence.
ACCESSION   DASRKK010000023 DASRKK010000000
VERSION     DASRKK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21105
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978279"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1191..2567)
                     /locus_tag="VFC15_00445"
     CDS             complement(1191..2567)
                     /locus_tag="VFC15_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZM08663.1"
                     /translation="MPAKPLETVDLTRRNFLSTSGRLLLLAPIASAFPWLNCSSIATE
                     PSKEAVASFPYTGTDEQLLDDVERTAFQFFWEQAHPRTGLVKDRARATGTDTYTVASI
                     ASTGFGLTALCIGDARGYMPTPAIRARVFATLDSVANLVAGHNGFYYHFVDWETGRRA
                     WKCELSSIDTSLLLCGVLTARQYFHNDSQIVALANKIYSGVQWPWMLNGGATLSMGWK
                     PESGFLDSRWNHYCELMMIYLLGIGSPSYPLAPATWKAWSRPTYTYQGITYISAGDPL
                     FTHQYSQAWYDFRNKRDDYADYFQNSVKATQAHRLFCLSLHDRFPTYTDNLWGIVSSD
                     SEHGYVAWGGPPAMGPIDGTVVPCAAGGSLPFVYADCVAVLRNMRGYKGVWGRYGFVD
                     AFNPMNGWVNADVIGIDVGITMLMAENQRTGLVWNTFMSSPEAQKAMQLAGFRELAQV
                     APALKKAA"
     gene            complement(2954..6454)
                     /locus_tag="VFC15_00450"
     CDS             complement(2954..6454)
                     /locus_tag="VFC15_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM08664.1"
                     /translation="MKSSRLLLLGLLVLFCCVIFTSAAQAQYRGSLRGTVSDPSGAVV
                     SGATVTLVNKDTNNTMVATSDGSGIYTFNGLPPAPYRITAEHPGFKKQVLEDVQIIPE
                     QLNSLNLELQVGTVDQTMTVSSTTEALDTETATISSTISSNQIQHMPSFNRDVFQLAQ
                     LTPGVFGDGSQQGGGGSFELPGNQGPGGTGGGNGGIFSTENGPQINTGGGQYENNGIS
                     IDGISTVSAVWGGTSVITPSEDSVGNLKVVSNNYDAEFGRFSGAQIQVSTKSGSNELH
                     GSAFFKASRPGLNAYQRWNGVGSTNPGTPAQRGLNRDENRFNQYGGSLGGPLWKNKIF
                     AFFNWETSPLSASSTSQGWYTTPQFANTAATPGSIAAKYLSFPGSGVASTGIIQRTCA
                     SIGLQEGVNCNTVSGGLDVGSPLTTGLGLQDLGYGGNSNVPGVGGGLDGIPDLAYYTL
                     VNPTSTSQMQFNGRLDANITHNDLLSFTIYWVPLSTTNYNGTVRPMNLWNHSQINDAF
                     ALIWTHTFSPSLLNQARANAAGWRWNEVTSNPQAPFGLPQDNIDNIGTANPAFFGAPG
                     PSNLNQWTYTYSDVLTKIIGRHSIKVGGDLTRLYYLNNPVYQARPGFNFHNLWDFAND
                     APYFENGQFDSQTGVPFANRQDGRQDLWGFFGQDDIKVLPTLTINLGLRWSDFGAFDT
                     KQNNLDVLQFGPGANPLTGLHIRVGGSLYTPQKTNFGPVVGFAWSPSKYGGNAVFRGG
                     FGISYNQNEIAITTNGSGNPPNAVQANFTCPYPFNSNPSCAGTGILYETATSPTSLFG
                     YAPNPNTITTFGPDNLPINGAAISVTGFPSNPKTIAVYHYSLDFQYQLPANWVMTMSY
                     MGNQTRHLLIHSNWNAIGAAAGLALNPKVNYLNFWDNNGDGNFNAGIFSLAHNFSHSF
                     QLSASYTWSKAMDENSGPYYQDPYPYSPSAAYGRSNYNVTNAFKLYGLWQPTFFHGSH
                     SWIARVADGWSLSGIWNLHSGFPWTPFYNINGNVYYSSSGYGNLRPAGMLPGAGTSTS
                     NSAFMQAINPNYGGNGTNFFVAPSYVPGPNFPAIGPAPLSGIQRNTLNAPGYNDVDLS
                     LSKAFGLPRIPGLGERAQFLVRVDVYNFFNKTNINSQSIDANLGSVAPNGTVTPNSDF
                     GVAGSALGSRTVQLQARFSF"
     gene            complement(7025..7918)
                     /locus_tag="VFC15_00455"
     CDS             complement(7025..7918)
                     /locus_tag="VFC15_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZM08665.1"
                     /translation="MYRLRNSVMVVLAFALCAMGQSAKSKRPKSEGEIVVQEIVISGT
                     STLTSEEVSDINNSLTSKTMGDDDEVVEAYITYEFQQRGYFDAEVTNLKVVALDPLAK
                     KKPVRIEAEVDEGPLYHLSQFKFTGNTAFSEDELRQGFPLHAGDVFDAEKVRLGIESL
                     AKKYWTTGYLETSLVPDTQKASSGKVTVTFAIAEGIQYRMGELRVEGKNEAAGKLKAK
                     WELKPGESYDLDYLEKFLSQNRELLPDGFDNDRDVLWLRDCSDDTVNVTIELDPKRQW
                     KPKPQDKPCDTEKDSPKGHIA"
     gene            complement(8006..9508)
                     /locus_tag="VFC15_00460"
     CDS             complement(8006..9508)
                     /locus_tag="VFC15_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08666.1"
                     /translation="MLKRFNLPWMLIALLLCTLPVLGQAPGEAIAIDASGPSRPFPHY
                     WEQMFGSGRAILTLRDSWRRDLREVKQITGLQFVRFHAIFHDEVGVYDEDKDGRPIYN
                     FSYVDQIYDGLLANGVKPFVELSFMPYKLAARPDIQAFWYRPNVAPPKDYAKWDDMIT
                     QFTRHLVERYGIDEVTSWYFEVWNEPNIDFWKGTPSQESYFELYDHSARDIKSVDRRL
                     RVGGPSTAQAAWVDAMIQHAVKLNIPLDFVSTHVYGNDTANDVFKTNEKIPRDQMVCR
                     AVKMVHDQIKASTRPDLPLIWSEFNAAYDNEVAVTDSIYMGPWMATTIGQCDGLTQMM
                     SYWSVSDVFEEQGVVKQPFYGGFGLIAAGGIPKPSFYAFEILHYLGDRRIDNSNRDVL
                     VTKAKDGSLVVAVWNLVNPGSTGSPRAVRLDFKGVSPDATASIARVDSQHGDTLDLYD
                     KMGKPRYPTQAQIQQLDRGSRLKSSEERHLKNGSLELQLPVNGLAVIQVR"
     gene            10657..11421
                     /locus_tag="VFC15_00465"
     CDS             10657..11421
                     /locus_tag="VFC15_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM08667.1"
                     /translation="MEDLKPHVILIAYHFPPGQEIGGFRPFRFCKYLQRMGYPCHVIT
                     ASPPAEENPDEVIYVPDDFRPIWEGWSQERLSLLGYAELLIRKCCFPAALGIMWSLKV
                     AAKCRQILRAHPRNRFVIFSTFPQLGVLFAGLIVRWREERPWICDLRDPIHGEPLFAH
                     ASRLTLFLNRHLEALAFRGASAVIANVEPAAAVWRDRYPLARQKIHTIYNGFDPDEMV
                     SAREIPPRKETVILHAGTVYLGRNPNLIVASLLRLR"
     gene            11503..11943
                     /locus_tag="VFC15_00470"
     CDS             11503..11943
                     /locus_tag="VFC15_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08668.1"
                     /translation="MDEAKREGLLKLVPAVLRQEAQRMTAEAHGLLLLQPQSTIQVPA
                     KLYEYICVGRPILALVPRSSAVEEILEKAGVRFVCVYTDDPPEDVDRKLVEFLRLPTQ
                     PSQYSDWFRDNFNAEYQAGQLSSIIEAIARSHGLIRVHIELHLA"
     gene            complement(12355..13665)
                     /locus_tag="VFC15_00475"
     CDS             complement(12355..13665)
                     /locus_tag="VFC15_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZM08669.1"
                     /translation="MLHLDTGNTGFMILCTSLVMLMTPGLAFFYGGLVGRKNVLAIMI
                     QSFVSMGWTTVIWYLYGYSLCFSGDWHGIIGNLHNVCLHGINLSTPSAPTDTIPAMVF
                     IAYQMMFAIITPALISGAFTNRVTFKAYCIFLTAWLTFVYFPFVHMVWGGGILQRWGV
                     EDFAGGIVVHNIAGMAALASILYVGRRRILDRGPHSIPLVALGTGLLWFGWYGFNAGS
                     EMRVDSVTATAFLNTDIAASFAAVAWLGVAWFRERKPKFLGLLTGAVAGLATITPAAG
                     YVSPTTAVIIGLVSGVVCYFAVEMKNKLHWDDALDVWGVHGVGGFLGILMLGLFANSA
                     FNPAGTNGLLYGNPSFFLKQLVAVLFSSVWAFGFTYAMLHIINYFTPVKVDEVSEEIG
                     LDESQLGEHAYETLEVEDVFNHDHQHVTIERFEVEEDELVGPRK"
     gene            complement(14028..14564)
                     /locus_tag="VFC15_00480"
     CDS             complement(14028..14564)
                     /locus_tag="VFC15_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08670.1"
                     /translation="MIIALAGRRVDAIDASQPRFPPQDIDKVSAAVRALLKQHGATAV
                     VSSAACGADLIGLAEAGKLGLRRRIVLPFGRTKFRESSVVDRPGDWGKMYDTILDDVE
                     ANGDLVVIDTGNDKEPYAVTNAAILDQAIVLGRERGVAVAATLVWDGISRGKDDYTDQ
                     FGAEARKRGLPVMEVPTI"
     gene            14645..15343
                     /locus_tag="VFC15_00485"
     CDS             14645..15343
                     /locus_tag="VFC15_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM08671.1"
                     /translation="MLLHPERWKSLRSRYEQDRPHRMLALDGGGIRGILTLGILQKIE
                     TLVAAKTGQKLCEYFDYIAGTSTGAIIAACLARGMTTAEIFAFYNSAGEQMFESSHLM
                     QRLRSLYTADPLKAKLQDVFGHDTTLEPAEVATEKSLKCLLLAVTKNFTTDSPWPVST
                     NPDAKYNDLSRKDCNLRIPLWQLIRASTAAPVFFPPEVLAWDPSDPAKTFVFVDGGIT
                     SHNNPGFLLYRMAT"
     gene            15422..15853
                     /locus_tag="VFC15_00490"
     CDS             15422..15853
                     /locus_tag="VFC15_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08672.1"
                     /translation="MGTTAASPNMNIVSNVAGIPGGLMNTIQIDQDINCRTVGRCTYG
                     EHLDSEVMDLVPRQVHEGMTRDEYYAAPPIPLSTDLGKRFLYCRYNIDLSSKGLGALG
                     FPKVDSASIQKMDAVENIPALTEIGIAAGRQVDAAHFGPFL"
     gene            15959..17791
                     /locus_tag="VFC15_00495"
     CDS             15959..17791
                     /locus_tag="VFC15_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZM08673.1"
                     /translation="MKHSEISRRDFLQRAGILSASLALPGDGVLAAGEVQPSVAPLAQ
                     FDYGDVTLNSELHQQQFDNTHAVLMGLSEDSLMKPFREMSGMPAPGEELGGWYMYKPD
                     YDYRKDSAGLCPGGTFGQWVSALARDYAITGDKATHEKVLRLNRLYVQSIASGFFEKN
                     RFPAYCYDKLVCGLIDSHEFAGDPKAFELLQQTTDIALPHLPEKAVDREQSWRAGKDI
                     SYNWDEAYTLPENLFLAYQRGAGVRYRDLAIRYLDDATYFDPLADGENVLPGKHAYSY
                     VNALNSAMQAYLTLGSQKHLRAARNAFEMLRTTQSFATGGWGPDEMLRKPDSGALADS
                     LSAIHKSFETPCGAYAHFKLTRSLLRVTRDSQYGDSMERVMYNTVLGAKPLQSDGTSF
                     YYSDYNFAGHKGYSNHRWPCCSGTLPQVAADYRINTYFYGADGLWVNLYIPSKLEWEI
                     PDVRGTLTQSTSYPFSDVVQFDLLVDHPQEFSLHLRIPAWTGEASVSVNGKRQAIDLR
                     PGSFAVLRRQWKSSDRVELELPMTTRLEAIDAQHPETVALMSGPLVLFAVTDSAAVVT
                     RKQLLAARKTGAQRWQVETVGVPMTMLPFTEIGDEGYSTYLRVP"
     gene            17909..20389
                     /locus_tag="VFC15_00500"
     CDS             17909..20389
                     /locus_tag="VFC15_00500"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZM08674.1"
                     /translation="MPEQPPQKPNVTVEDDRTGTSVTTLKQAILDNLYYIVAKTTANA
                     TESDYFAAVAYTVRDRMLSQWIATLDSYSREELRVVCYLSAEFLMGPYLANNILNLGL
                     TGPFRQAIEELGLDFDRIIEQESEPGLGNGGLGRLAACYLDSLATLGVPAIGYGIRYQ
                     FGMFHQQIRDGWQVEMTDYWLRYGNPWEIRRPQASVDVGFGGHTEPYTDAKGNYRVRW
                     MPRSVVKGIPYDTPIVGYRSGVVNDLRLWKSEATESFYFERFNSGDYQGAVAEKNFAE
                     NITKVLYPNDEEIQGKELRLQQQYFFTSCSLQDMIRFQHRMGCPLETFHKEWAIQLND
                     THPSIGVAELMRLLLDEHDMGWDQAWSITTQTFSFTNHTLLPEALERWPLPLFGSLLP
                     RHLEIIYEINARFLTEVRERFPGDEARVARMSIIDEHDQKYVRMAHLACVGSHKVNGV
                     AELHTQLVKQELFHDFYQMWPEKFVNVTNGVTPRRWLAVSNPEQSALMTSKIGDGWIR
                     NLEELKQLQGLADDSAFRAEWRKVQRDVKVRLSNYILKCSGQSVDPSSMFDAQVKRIH
                     EYKRQHLNVLHIITLYLRLKSNPNLEITPRTFLFGGKAAPGYVMAKLIIKFINSVGEE
                     VNNDPDVKQRLKVVFLPDYDVSLGQKVYPAADLSEQISTAGKEASGTGNMKFAMNGAI
                     TIGTLDGANVEIREAVGAENFFLFGLTASQVSSTLAAGYHPWDIYQSNAGLREAIDSV
                     ASGQFSHGDRELFRPLVDSLLQHDPYLLFADYQAYIDCQDQVARAFGDQENWSRMAIL
                     NALRMGKFSSDRSITEYCEGIWNVAVKA"
     gene            complement(20436..>21105)
                     /locus_tag="VFC15_00505"
     CDS             complement(20436..>21105)
                     /locus_tag="VFC15_00505"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZM08675.1"
                     /translation="CKQRRLGASPPPTQRARNDSVPFIFDSRVIDLQIDTPPTANWHI
                     MPVTKQEAGMAHCTKCGAELPDNAQFCQKCGQPQVAPPPNPGFRPPSPPYQPATAGLS
                     ENAAATLSYVLGWLTGIIFYLIDKRPYVRFHAAQSIVTFGGLSIIHRVVAIMFGLGWA
                     FGGFSPFGGIGHFAGFGFGIMLLSLIGLVMFVLWIVCVIKAYQGERFMVPIAGDIAAN
                     LSGH"
BASE COUNT         4588 a         5929 c         6050 g         4538 t
ORIGIN      
        1 gttttgtgcc cactggcagt ttgtatgcgc ttggatcgta gcgaagcaag aagcgagaaa
       61 cccgcatgaa tactagctag tctcactcca tacaaactgc gctaagttgt tgattcttgg
      121 ttccacaagt actcttaatc agtaggttga aggttcgatt ccttccgcgc tcaccacaac
      181 tcccctgtaa atatgagatc ttttggcata ccaccacgca attgcgaatt gctgcctgtc
      241 attcttgtca ccgctgcgtc accaaatctt gggatcctgc actggcgtca ccgaccgttt
      301 gccgtacgtc gggtttcacg taacactgag gtgagcccgg aatgggggca gtctgtaccc
      361 gcaggtcatg ctgtgtgtca gaacccgtgg caatggatga acttcgtgcc ttcctccagc
      421 aatcaaactg gacgttcgcc aaaaccatga ccacgactcc gctcgcgtac acccttcggg
      481 tcccggcaac ggacgaggcg gtttcgaagc ccggatcacg caggcgagtc cggcccctga
      541 agccatggtc tgagtctgca ccgacacccg ggagtccttg cataagtgca tgacgggaag
      601 taacttgcgc gcctcgacta cttttctttg acggtttaga aaacggtcat ccacgcctcg
      661 ttgcggcttc gggtattcgt gtgcgacgac atggcattcc acggcgacta cacgccgagg
      721 acgatgtttc cgaatctcaa cattccggag ttcctgaaat cgggttcggg gacaggtttt
      781 cggccctaaa gtgacggcgg agcagtggag ccaggaaggc aagcagggcg tgaaaatgac
      841 gcggctatcc tgccatcgat tccgctcgaa ccaagtccga caagtattga gcctcttggt
      901 ttacaactcg gggaatccgt gccgtcgact ggtactcccg cagaggatta agaattggtc
      961 gttgacgagt ctcgcaacaa cgactggtga agacggggga ggactgccga aacatgcccg
     1021 ccattattgg ctacagttgg cggagagtca tttcacgcgg ccgctcctaa aggccactct
     1081 accaccgagg ccacttattc agagtttccc tagtcttggg tagccagccg cacccgtggg
     1141 taacctcatc cgcactctca aggaacctca tggagcggca ccctgcgcgt tcatgccgct
     1201 ttcttcaatg cgggggcgac ttgagcgagt tcccgaaatc cggccaattg catagctttc
     1261 tgtgcttccg gactgctcat gaaggtattc cacaccagcc cggttcgttg gttctcagcc
     1321 atcaacatgg tgatgccaac gtcgattccg attacgtcgg cgttcaccca gccgttcatg
     1381 ggattaaatg catcgacgaa tccgtaacgc ccccagacgc ccttatagcc acgcatattc
     1441 cgcagcacgg ccacacagtc ggcgtagaca aatggcagcg acccgcctgc ggcacaagga
     1501 acgaccgtgc catcgatggg tcccattgcc ggcggaccgc cccacgccac gtatccatgt
     1561 tcggaatcag acgacacgat tccccagaga ttgtcggtat aggttgggaa tcgatcatgc
     1621 agcgagagac agaagagtct gtgtgcttgc gtggccttca ctgaattctg gaaatagtcg
     1681 gcatagtcgt cacgtttgtt gcgaaagtca taccacgcct gcgaatactg atgggtaaac
     1741 agcggatcgc cggcagaaat gtaggtgatt ccctgatagg tgtaggtggg tcgcgaccat
     1801 gccttccagg ttgcaggagc cagcggatag ctgggggatc cgatgcccag caggtagatc
     1861 atcatgagct cgcaatagtg gttccagcgg ctgtccagaa agcccgattc cggtttccat
     1921 cccatggaca gcgtcgcgcc accattcaac atccacggcc attgcacgcc ggaatagatc
     1981 ttgttggcga gagcgacgat ctggctgtcg ttgtgaaagt actggcgcgc cgtcaacacg
     2041 ccgcaaagca gcagggacgt gtcgatcgat gacaactcgc acttccaggc acgccgtccg
     2101 gtctcccagt caacgaagtg ataatagaag ccgttgtgcc ccgcgacgag gttagcaact
     2161 gagtcgagag tggcgaatac gcgcgccctg atggctggag ttggcatgta tccgcgggca
     2221 tcgccaatgc agagggccgt cagtccgaag ccggtggagg cgatgctcgc aaccgtgtag
     2281 gtatcggtgc cggtggcccg ggcgcgatcc ttgaccaacc cggtgcgcgg gtgcgcctgt
     2341 tcccagaaga attgaaatgc agtgcgttcg acgtcgtcga gcagttgctc gtctgtgcct
     2401 gtgtacggaa agctggctac ggcttccttc gacggctcgg tcgctatgct ggagcaattc
     2461 agccagggaa aggcactggc gatcggagcc agcagtagga gccgtccgct cgtgctcaga
     2521 aaattccgcc gagtaagatc aacagtctcg agtggctttg ccggcatcgt ggaacaaatc
     2581 cccctgcatt tccaataagc ccagcggacg taattcctag gcagcattcg ctggactgct
     2641 gctcaactcc aaacctctaa atattccatg aaaactgttg ctgctgttcc tggcaggcgg
     2701 cgctcggaag gagcagccgc acggcgcgtt ttcgtcgcgc agggtggatc tgcataacga
     2761 tagcttgaat tccggacgcc ctcccccgtc cggccctttc ccaacgggtg aatcggaaga
     2821 caggcccggc tggcaacgag ggttagcctc cgcctcagcg ttctccgccg gcccgctcca
     2881 gcttttcaat ccagaatgaa ggcttcctct ggcggggcac tcggaaatac gggtacccgt
     2941 gccaggtgaa aagttagaag ctgaacctcg cctgcagttg caccgttcta ctgcccaatg
     3001 cgctgccggc cacgccgaag tccgagttcg gagttaccgt gccgttcggc gccaccgaac
     3061 ccaggttcgc gtcgatagac tgagagttaa tgttcgtctt attgaagaag ttgtaaacgt
     3121 ccacacggac caagaactgc gctctctctc ccagccctgg gatcctcggc aggccgaaag
     3181 ctttgctcaa gctgagatct acgtcgttat agcccggagc gttcaaggta ttgcgttgga
     3241 ttccagacag cggcgcaggc ccgatggccg gaaagttcgg ccctggcaca taactcggtg
     3301 ccacgaaaaa gttggtaccg ttgccgccat aattcgggtt aatggcctgc atgaatgcgc
     3361 tgttactggt gctcgtgccc gcgccaggga gcatccctgc gggccgaagg ttgccgtaac
     3421 cgctgctcga gtaatacaca ttgccgttga tattgtagaa aggggtccag gggaagcccg
     3481 aatgcaggtt ccatatgccg cttaaactcc acccgtccgc cactcttgca atccagctat
     3541 ggctgccgtg gaagaacgta ggctgccaca ggccgtacag tttgaacgca ttggtcacgt
     3601 tatagttaga acgtccatac gccgcgctcg gactgtaagg gtacggatcc tggtaatacg
     3661 gacctgaatt ctcgtccatg gccttggacc aggtatatga cgctgatagc tgaaaactat
     3721 gcgagaaatt gtgcgcaagg ctgaagatcc ccgcattgaa gttcccatcg ccattgttat
     3781 cccagaagtt caggtaattg accttcggat tgagcgccag ccctgccgcc gcgccgattg
     3841 cattccagtt gctatgtatc aaaaggtgcc gtgtctggtt gcccatgtaa ctcatggtca
     3901 tcacccagtt ggccggcaat tgatactgga agtccagtga atagtggtag acggcgatgg
     3961 tcttggggtt cgagggaaaa ccggtcacag agattgccgc cccattgatc gggaggttgt
     4021 cggggccaaa ggtcgtgatc gtattggggt tgggtgcgta tccaaagaga gaggtaggac
     4081 tggtggcggt ttcatagagg attccggttc cagcacagga tgggttgcta ttgaagggat
     4141 aggggcaggt gaaatttgcc tgcaccgcat ttggaggatt gccgctgccg ttggttgtga
     4201 tggcaatttc gttttggttg tagctgatgc caaagccccc gcggaacacc gcattgccac
     4261 catacttact gggcgaccag gcgaatccaa ccaccggtcc aaagtttgtt ttttgcgggg
     4321 tgtagagact tccacctacg cggatgtgga gacctgtcag tgggttcgca cccggtccaa
     4381 actgcagcac gtccaggttg ttctgcttag tgtcaaacgc tccgaagtcc gaccagcgca
     4441 gaccaaggtt gatggtcagg gtcggaagga ctttgatatc gtcctgccca aaaaagcccc
     4501 atagatcttg gcgaccatcc tggcgattcg cgaaggggac acccgtctgg gaatcgaact
     4561 ggccattttc aaaataggga gcgtcgttgg caaaatccca cagattgtgg aaattaaagc
     4621 cgggccgcgc ctgatagaca gggttattca agtaatagag ccgggttaaa tcgccgccga
     4681 ctttgatgct atgacgtccg atgatcttgg tgaggacatc actgtaggtg taggtccact
     4741 ggttaaggtt gctgggtccg ggggcaccga agaatgcagg atttgcggtg ccgatattat
     4801 cgatattgtc ctgcgggagt ccaaacggcg cctgcggatt gctggtaacc tcattccacc
     4861 gccaccctgc ggcgttggcg cgcgcctgat tcagcaagct gggcgagaaa gtgtgcgtcc
     4921 agatcagcgc aaaggcatcg ttgatctgtg agtgattcca gaggttcatc ggacggacag
     4981 tgccgttgta attcgtcgtt gacagcggca cccagtagat ggtgaagctg agcaggtcat
     5041 tatgggtgat gttggcgtcg aggcggccgt tgaactgcat ctgcgatgta ctcgtcgggt
     5101 tcaccagggt gtagtaggcc aagtcgggta ttccatccag gcccccgcct acgcccggga
     5161 cattggaatt gccaccatag cccagatctt gtaagcccaa gccggtcgtc aagggcgagc
     5221 ctacatccaa gccacccgat acggtgttgc agttcacccc ttcctgcaac ccgatggatg
     5281 cgcaggtacg ctgaatgatc ccggtcgatg ccacaccgct tcccggaaac gaaaggtact
     5341 ttgccgcaat gctacccggc gtcgcagccg tgttagcaaa ttgcggtgtc gtataccagc
     5401 cttgagacgt actagaggcc gaaagcgggc tggtctccca gttgaagaag gcaaatatct
     5461 tgtttttcca aagcggtccg cccagactgc cgccgtattg attgaatcgg ttctcatcac
     5521 ggttcaggcc acgctgtgct ggcgtgcccg ggttagtgga accgacgccg ttccagcgct
     5581 ggtaggcatt cagtcctggc cgtgatgcct tgaagaaagc gctgccgtgc agttcattgc
     5641 tgccgctttt cgtgctgacc tgaatttgcg cgccgctgaa ccggccgaac tccgcgtcgt
     5701 aattgttgga gacgaccttc aaattgccca ccgagtcttc gctgggggtg atcactgacg
     5761 tgccgcccca caccgcgctg accgtgctga tcccgtcaat actgatgccg ttgttctcat
     5821 actggccgcc gcccgtgttg atctgcgggc cgttctccgt cgagaaaatt ccgccgttac
     5881 ctccgccggt cccgcccggt ccttggttcc caggcaactc gaaggacccg cccccgcctt
     5941 gctgcgagcc atctccgaaa acgcccggcg tcagttgcgc caactgaaag acgtcgcggt
     6001 tgaacgacgg catgtgctgg atctggttgc tggagatcgt gctgctgatg gtggccgttt
     6061 ccgtgtccaa cgcctcggtt gtgctcgaca ccgtcatggt ttggtccacc gtccccacct
     6121 gtaattccag gttcagcgaa ttgagctgct cggggatgat ttgcacatcc tccagcacct
     6181 gcttcttaaa tcccgggtgc tccgcggtga tccggtaggg cgccggcggt agcccgttga
     6241 aggtgtaaat gccacttccg tcggaggtgg ctaccatcgt gttgttagtg tctttgttca
     6301 ccagagtcac cgtcgcgcca gaaaccactg cgcccgaggg atcactcact gttccgcgaa
     6361 gggaacctcg atactgcgcc tgggcggcgg atgtgaatat tacgcagcaa aacaaaacca
     6421 acaaaccgag tagcagaagc cttgaactct tcatgacatt ttcctccggc gtgcaatggt
     6481 ttcaggcgaa caggtgtgcc tggagcaaac aaggaattca cgtttgtgaa gaatcgattg
     6541 atgtccctgg tgagtttcca ggcgttcggt tgagatcgag ttttggccag aaacgaaact
     6601 ttcagtgaga gagagcctag gcttggcagc cgacttgcgg tcggctgagc ggactgggaa
     6661 tccggttacg ccaacaacgt gggtacacat ctgtcatcca tcgcgaacaa acgatgtacg
     6721 ggtactgctc ggcgcataat agcacttctg tcgtgacgcg tgtcactttt ttctgtgagc
     6781 tgctagcacc aaggtgagcg ggttaggtgg ggcttttaac tgctgccgtc gcgatgcatc
     6841 accgcgggat ctccagcaag accagcctgc agcgatacgg agatatttcg cggtgaccga
     6901 acttttagcc gcgaagcggt ggtttggggt aagcccagag taccgctggg aaataacccg
     6961 agccgcggag cggcggcact caggaaccca aaggctatct cccgccccat agtcccgtgt
     7021 gcaatcaggc gatgtgaccc ttcggactat ccttctccgt atcgcacggc ttatcctgcg
     7081 gcttcggttt ccactgccgc tttggatcga gctcgatcgt cacgttgacg gtgtcgtccg
     7141 agcagtcgcg cagccacaga acgtcgcggt cgttatcaaa tccgtccggc aacaattctc
     7201 ggttctgcga aagaaacttc tcaaggtagt cgaggtcgta ggattcgcct ggcttgagct
     7261 cccacttcgc cttcagtttc cccgcggctt cattcttgcc ttcgaccctc agctctccca
     7321 ttcggtactg gatgccttcc gcaattgcga aggtcacggt aaccttcccg ctggacgctt
     7381 tctgcgtatc aggcaccaga gaggtttcaa gataacctgt ggtccagtat ttcttggcca
     7441 gactctcgat gccgaggcgc accttttcgg cgtcaaatac gtctcccgcg tggagcggaa
     7501 atccctgccg caactcgtcc tcgctgaagg ccgtatttcc cgtgaacttg aattgggaga
     7561 gatggtacag cggtccttcg tcaacctccg cttcaatgcg caccggcttc ttcttcgcca
     7621 gcggatcgag cgcaacgacc ttaaggttcg tgacctcggc gtcgaagtat cctcgctgct
     7681 ggaactcata ggtgatgtac gcttccacta cttcgtcgtc atcgcccatc gtcttggagg
     7741 tcaggctgtt gttgatgtca ctgacctcct ccgacgtcag cgtggaagtg ccggaaatca
     7801 cgatttcctg cacaacaatt tcgccctctg acttcggacg tttggacttg gcactctgac
     7861 ccattgcgca cagagcgaat gccagtacga ccatcacgga attgcgtagc cgatacacgg
     7921 ggcccctccc ttcgagccag ggagtcgact aacgataccc tccgcagact gaaacgagaa
     7981 tctgctgtcc tacaacgtct gccgcctatc gcacctgtat tacagccaga ccgttcaccg
     8041 gcaactgcag ttccaggctg ccattcttca agtgccgttc ctcggaagac ttcagcctgg
     8101 atccccgatc cagttgctga atctgcgcct gggtcgggta gcgaggcttc cccatcttgt
     8161 cgtacaggtc caaggtgtcg ccgtgctgac tgtcgacacg ggcgatcgac gccgttgcgt
     8221 cggggctcac tcctttgaag tcgagtctca cggcgcgcgg cgagcctgta gaaccgggat
     8281 tcaccaggtt ccacaccgca accaccagcg agccgtcttt tgccttagtc accagcacat
     8341 cgcgattgct gttgtcgatc cgtcgatcgc cgagataatg caaaatctca aaagcgtaga
     8401 acgatggctt cgggataccg cccgccgcaa tcaatccgaa gccgccgtag aatggctgtt
     8461 tcaccacgcc ctgctcctcg aatacgtctg acacgctcca gtaggacatc atttgcgtca
     8521 agccgtcaca ctggcctatc gtcgtcgcca tccatggccc catgtagatc gagtcagtca
     8581 cagcaacctc gttatcgtat gcggcattga actcgctcca gatgagcggc aggtccggac
     8641 gcgtcgatgc cttgatctgg tcgtgcacca tctttaccgc gcggcagacc atctggtcgc
     8701 gcggaatctt ctcattcgtc ttgaagacgt cgttggcggt gtcattgcca taaacgtgcg
     8761 tggaaacaaa gtcgagcgga atgttcaatt tgaccgcgtg ttggatcatg gcatcgaccc
     8821 acgccgcttg cgccgtggat ggacctccaa cgcgcagccg acggtccacg gactttatgt
     8881 cacgcgcgct atggtcgtac agttcgaaat acgattcctg gctgggcgtc cccttccaga
     8941 agtcgatgtt gggttcgttc cacacttcga aataccacga ggtgacttcg tcgatgccgt
     9001 aacgctcgac caggtgccgg gtgaactggg tgatcatgtc gtcccacttg gcgtaatcct
     9061 tcggcggtgc gacattgggg cgataccaaa atgcctggat gtctgggcgc gcagcaagct
     9121 tgtagggcat gaaactgagt tcgacaaacg gcttgacgcc gttggccaaa agaccatcgt
     9181 aaatctggtc aacgtaggaa aagttgtaaa tcggccgacc gtctttgtct tcgtcgtaaa
     9241 cgccgacttc gtcgtggaag attgcgtgga agcgcacgaa ctgcaagccg gtgatttgct
     9301 tcacttcgcg cagatcgcgg cgccaggaat cgcgcagagt gagaatggcg cggcccgaac
     9361 cgaacatctg ctcccaataa tgcggaaagg gacgcgatgg ccccgaggcg tctatcgcaa
     9421 tggcctcgcc cggcgcttgt ccgagtacgg gaagcgtaca aagcagtaaa gcaatcagca
     9481 tccatggcaa attgaatcgc ttgagcatcg ttgtctccag atcgtagggt ctagcaggtc
     9541 gttcttaatt aggttgcaac ccggggtgca ccttccggtg tagcatttca aacaagagac
     9601 agacaaccgc gtaagtcgtg gcgtttatcg gaggaaccca ccagaaatgc agaggaacat
     9661 ggcgggcaaa aaagagaatc ggacaggtta taaaaccggc ctgaacgact ggctctctca
     9721 aactccttag gaggaaatcg gacggcgcta cgaacgaata aataacccag caacacgcaa
     9781 cgagaaaccc aacgcttgcc caactcgtaa ttcggcattt catggcctgc ctccgagagt
     9841 tgtcgttacg ataaggtcgg acgtgagttt accactgatc agttctgaga aatgcgtact
     9901 tcaagacgag aatagtgaac gctaaatcgc caagccactg gcgatccgcg ctgagaatcc
     9961 cggcttcacg gttgggagtg ctgcccctgt taacagaagc tattctgccc ctactggtta
    10021 cctcgcacta aactcagacg gcccaggccg ttcgcgggat gcttcgcctg ctcacgctca
    10081 ctcgtgaggc aaatcactta agcccccacg ctccgacagt acactgtccg cgcatgccgc
    10141 cgactcaacc gacgccgact gagaaacact ttttcacccg atgggttgta gccagctctg
    10201 taggatgggt ctttgcagta atcgtcgtcc tcatgctgac tgaactccaa gatttgttca
    10261 ccgtcggcaa ctgggtttac atcggggttg ggatggggtg gagcatcggg tttgggcagt
    10321 ggcgtattgc ccgcaagtgg ttcggtgcga caagtcgatg gatgtgggcg tccaccatcg
    10381 gcgtaacgat accgttcctg ctcgcagacc tcgttggaac ccaatgggac cgcggtcgca
    10441 ggtacctcat accctccctt gctgctgcgg gaggcctgct ctcggggtta ttgcagagaa
    10501 gcagcctgcg ctcgctgacc tgtcgggcca accaccgccc gttaacgaca agggccgtcc
    10561 cgtacgaaca tggcagcggc cagtgccacg ctcccaaatg ggaggcagag tcacgtgttt
    10621 gcttcaactg ggctaacata atcgcttggc ctgtccatgg aagatctcaa accccatgtc
    10681 attctgattg cctatcattt tcctcccggt caagagattg gtggctttcg gccgtttcgg
    10741 ttctgcaaat atctgcagcg catgggatac ccgtgccatg tgattacagc cagtcctcca
    10801 gcagaagaaa acccggatga ggtgatttac gtgcctgatg actttcgccc catatgggag
    10861 ggatggagtc aagaaaggct ttccctgctc ggttatgcag aactgcttat tcgaaagtgt
    10921 tgcttccccg cggctctcgg aatcatgtgg tccctcaaag ttgcagccaa gtgtcgccag
    10981 atcctccgcg ctcatccgag gaatcggttt gtcattttct ccacctttcc ccagctggga
    11041 gtgttgttcg ctgggctgat cgtacgatgg cgtgaagaaa gaccttggat atgcgatttg
    11101 cgcgacccga tacacggtga accactcttc gcgcatgcgt cgcgcctcac cctatttctg
    11161 aaccgccacc tggaggcctt ggcatttcga ggcgccagtg ctgtcattgc gaatgttgag
    11221 ccggcagcag ccgtctggcg cgaccgctat cctttagccc gacagaaaat ccatacgatc
    11281 tacaacgggt tcgatcctga tgaaatggtt tctgcccgcg aaatcccgcc ccggaaggag
    11341 acggtgatcc tgcacgccgg cacggtttac ctgggcagga accctaactt gatcgtcgcc
    11401 tccctattac gattgcgcta gcgacaagcc ccagaggcgg cctcagtttg cctttatctg
    11461 atgggcaatg tcggacccaa ctcgggtcag gattcggttc tattggacga ggcgaagcgc
    11521 gaagggctgc ttaagctggt gcccgctgtc ctccggcagg aggcgcagcg catgaccgcg
    11581 gaggcacatg gattgctatt gctgcagcct cagagcacga ttcaagttcc ggccaaacta
    11641 tatgagtata tttgtgttgg caggccaatt ctggccctag ttccgcgctc ctctgccgta
    11701 gaggaaatcc tcgaaaaagc cggcgtgcgt ttcgtttgcg tctacaccga tgaccccccg
    11761 gaggatgtcg accgaaagtt ggtcgaattc ctgcgccttc caacgcaacc cagtcagtac
    11821 agcgattggt ttcgggacaa cttcaatgcc gaatatcagg ccggtcaact ctcgtccatt
    11881 attgaagcga tcgcgcgaag tcacggtcta atccgcgtcc atattgaatt acatttggca
    11941 tgagcggagt ttcctccatg atctacacgg caagactgtc cgtgcgaatc tgagtcgatg
    12001 ctaagacggg agaactcatg tttttgttcg gtgcccggtc atggtcgaag cacgggcgtt
    12061 ttcaaagttg gtgtgtccta tagccacagt tgtaaaacca tgtgggtgcg ttctgggggt
    12121 tggcggcggc catgtctgct gtcgtcgttt gctcgaagac gtcatacagg tggccacttc
    12181 cacgattcat tccgaagtac tgaactgaca gaggcgcatt gcggccccgg gatacagggc
    12241 ggactgcaac ctgtgcgctg ccacacatgc cggttgccgg caggaggata ttacattctc
    12301 ctgccgacac cctcttccaa gccgaccgtg aggtccacga ccctcgggat gcaactactt
    12361 tctggggccg accaactcat cctcttctac ttcgaaacgc tcgatcgtca catgttgatg
    12421 gtcgtgatta aatacgtcct cgacttctag agtctcataa gcgtgttcgc cgagttgcga
    12481 ctcatcgaga ccgatctcct cactcacttc gtccacttta acgggagtga agtagttaat
    12541 gatgtgcagc atggcatacg tgaacccgaa ggcccacacc gatgagaaca gcactgcaac
    12601 cagttgtttc aggaagaagc tgggattgcc gtacagcaag ccgttggtcc cagccggatt
    12661 gaatgcgcta ttggcgaaca gccccagcat caggatgccg aggaagccac cgactccgtg
    12721 aacgccccac acatccagcg cgtcgtccca gtgcagtttg ttcttcattt caaccgcgaa
    12781 gtagcaaact acgcccgaga ccaggccgat gatgactgcg gtggttggcg agacatatcc
    12841 cgcagcaggc gtgatggttg cgagccctgc gacggcgccg gtcaggaggc cgaggaactt
    12901 cggtttgcgc tcacggaacc atgccactcc caaccaggct acagcggcga acgacgcggc
    12961 gatatcggtg tttaagaacg cggtcgccgt aaccgaatcc acccgcattt cgctgccggc
    13021 gttaaagcca taccaaccga accacaatag cccggtgccc agagccacca gcggaatgct
    13081 gtgtggaccg cgatccagaa tgcggcgccg gccgacataa aggatggacg cgagggcggc
    13141 catgccggca atgttatgca ccacgatgcc gccggcgaag tcctctacac cccagcgttg
    13201 taggatgcct ccgccccaga ccatatgtac gaaggggaaa tagacgaagg tgagccacgc
    13261 ggtcaggaag atgcaatagg ccttgaaggt cacgcgattg gtgaacgcgc ccgagatgag
    13321 tgccggggtg atgattgcga acatcatctg gtaggcaatg aagaccattg ccggaattgt
    13381 gtcggtcggc gcggaaggcg tggagagatt gatgccatgc agacagacgt tgtgcaggtt
    13441 gccgataatt ccgtgccagt caccgctgaa acacagcgag taaccataga ggtaccagat
    13501 gaccgttgtc cagcccatcg agacaaagct ctgaatcatg atggcgagga catttttgcg
    13561 gcccacaagg ccgccgtaaa agaacgccag cccgggggtc atgagcatga ccaggctggt
    13621 acaaagaatc atgaatccgg tgttaccggt gtcaaggtga agcatgaaag cctccagaaa
    13681 gagatatgcg gcgaaggaca ggcgcggcgc tcagggagaa gccacgctca attgacgctc
    13741 caacagaatt gtgtggagtc gcaaggaagg gggagcaatg atctgcgatg ccttgggtag
    13801 gcaaatcgcg cttcacgttc aacgcgagaa agtttcgaga cgagacaacc aataatggca
    13861 atggaaacgt actttgcctc gcagcagcgt ccactaacta aggagacgtc aaaatgcccc
    13921 tataacaggc tgcgcttcgc gggcgttgaa atctgtgctg ctagtcaccg gattgggtga
    13981 cattgggcac gtggaaggac cacctggcaa atcccggggt tggggcttca gatggtgggg
    14041 acctccatca ctggtagtcc ccgtttgcgg gcctcggcgc caaactggtc ggtgtaatca
    14101 tcttttcctc tggagatgcc atcccagacc agcgttgcgg ctaccgcgac gccgcgctct
    14161 cgtcccaaga cgatcgcttg gtcgaggatt gccgcattgg tcactgcata gggctctttg
    14221 tcgttgccgg tgtcgatgac gaccaggtcg ccgttggctt cgacatcgtc gaggattgtg
    14281 tcgtacatct ttccccaatc accgggacgg tcaacgactg aggattcacg aaactttgtg
    14341 cggccgaacg gcagcacgat ccggcggcgt agccctaact ttcccgcctc tgccagaccg
    14401 atgaggtccg caccgcacgc agccgagcta acaaccgcgg tcgcgccatg ttgcttcagc
    14461 agagcacgta cagccgcact taccttatcg atgtcctgcg gcgggaacct tggttgactg
    14521 gcgtcaatgg catcgactct gcgtcctgca agcgctatga tcatcttccg ttttccactg
    14581 tcgtatagtc tcgcacaata ggacaaccgg cagcccatat cgccgccaaa taggagagtt
    14641 cccaatgctg cttcatccag aacgatggaa atcactgcgt tcccgttatg aacaagaccg
    14701 gccacaccgc atgttggccc tcgacggcgg cggcattcgc ggcatcctca cactcgggat
    14761 tctgcaaaag atcgagacgc tggtggcggc aaagaccggc cagaagctct gcgagtactt
    14821 tgattacatt gccggaacca gtacgggagc gatcatcgcc gcctgtctgg cccgcggcat
    14881 gaccaccgcc gaaatattcg ctttctacaa ttccgccggc gagcagatgt ttgaatcatc
    14941 acatctgatg cagcgactgc ggtctctcta tactgccgat cccctgaagg ccaagttgca
    15001 ggatgtcttt ggtcacgaca cgactctgga gccggcagaa gtcgccaccg agaagagtct
    15061 gaaatgcctg ctgctcgcag taacgaaaaa cttcactacc gattcgccct ggccggtcag
    15121 cactaatcca gacgccaagt acaacgacct cagtcgcaaa gattgcaact tgagaatccc
    15181 gctctggcaa cttatcagag ccagcactgc cgcaccggtc ttctttccgc cggaagttct
    15241 ggcttgggac ccgagcgatc cggcaaagac cttcgtgttc gttgacggtg ggataacttc
    15301 ccacaacaat ccaggattct tgctttaccg catggcaact taaccggcgt atcgactcaa
    15361 ctggcgcacc ggcgaaaaga acctgctgct ggtttccgtt ggaactggag ctgcggaggc
    15421 catgggcacg accgcggcgt cgccgaatat gaacatcgtg tcgaacgtcg ccggaatccc
    15481 gggcggcctg atgaacacca tccagattga tcaggacatc aattgccgca cggttggccg
    15541 ctgcacctac ggcgagcatc tcgacagcga agtcatggac ctggtgcctc ggcaagtgca
    15601 tgagggtatg acgagagatg agtactatgc ggcgcccccg attccgctct ccaccgactt
    15661 gggcaaacgt tttctgtact gccgctacaa tatcgatcta agcagcaaag ggcttggggc
    15721 gctagggttt ccgaaagtcg attctgccag cattcagaag atggatgcgg tcgagaatat
    15781 tcccgcgctc accgagatcg gcattgcggc cggacggcag gtggatgcgg cgcattttgg
    15841 accatttctc tagaagccgc gtagggtttc tagctggcgg atgggtccgg ggagagaccc
    15901 tccagcgcga gcggtcagat tctggcgcat ctctattggt ggacaatgtt ggggcggtat
    15961 gaagcattcg gagatttcgc gacgcgactt tttgcaacgc gctggaatat tgagcgcaag
    16021 tcttgcgcta cccggcgacg gggttttggc agcgggagaa gtgcagccaa gcgtcgcccc
    16081 gctggcgcag tttgactacg gcgatgtgac gctaaacagc gagctgcatc aacagcagtt
    16141 cgacaacact cacgcggtat tgatggggct gagcgaagac agtctgatga agccgtttcg
    16201 cgagatgtcg gggatgccgg cgccgggcga ggaactgggc ggctggtata tgtacaagcc
    16261 agattacgat taccgcaaag atagtgctgg actgtgtccc ggcggcactt ttgggcagtg
    16321 ggtgtcagcg ctggcacggg attatgcaat caccggcgac aaagctacac acgaaaaggt
    16381 cttgcgccta aaccgcctct acgtgcaatc gatcgcgagc ggatttttcg agaagaaccg
    16441 cttccctgct tactgctacg acaagctggt ctgcggcctg atcgactcgc acgaattcgc
    16501 aggagatcca aaggctttcg aacttttgca acagaccacc gatatcgcgt tgccacacct
    16561 gcccgaaaag gcggtggacc gcgaacagag ctggcgggct ggtaaggaca tttcgtacaa
    16621 ctgggacgag gcgtacacgt tgccggaaaa cttgtttctc gcctatcagc ggggcgcggg
    16681 cgtccgatac cgcgatcttg caatccgtta cctggacgat gcgacatact tcgatccgct
    16741 ggccgatggg gagaacgtgc tcccaggcaa gcacgcctac agctacgtga acgcgctgaa
    16801 ctcggcgatg caggcttacc tgacgctggg cagccagaag cacctgcggg cggcgcgcaa
    16861 tgcctttgag atgctacgga cgacgcagag cttcgcgacc ggtgggtggg gcccagacga
    16921 gatgctgcgc aagccggata gcggggcgct ggccgacagc ctgagcgcca tccacaagag
    16981 cttcgagacg ccgtgcggtg cttacgcgca cttcaagctg acgcgctcgc tgctgcgcgt
    17041 cacccgcgat tcccaatatg gcgacagcat ggagcgcgtg atgtataaca ccgtgctggg
    17101 tgcgaagccg ttgcagtcgg atgggacatc gttttattac tccgattaca atttcgcggg
    17161 acataagggt tactcgaatc atcgttggcc gtgctgttcg ggaacgctgc cgcaggttgc
    17221 ggcggactat cgaatcaaca cgtactttta cggtgccgat ggattgtggg taaacctgta
    17281 cattccgtcg aagttggagt gggagattcc agatgtgcgc ggaacgctga cacaaagcac
    17341 cagttatccc ttctccgacg tcgtgcaatt cgatctgttg gttgaccacc cgcaggaatt
    17401 tagcctgcat ttgcggatac cggcgtggac gggagaagca tcggtctcgg tgaacggcaa
    17461 gcgtcaggca attgatctga ggccgggtag ctttgcggtg ctgcgccgac agtggaagtc
    17521 gagcgaccgg gtcgaacttg agttgccgat gactacacgg ctggaagcca tcgacgcaca
    17581 gcatccggag accgtggctc tgatgagcgg gcccttggtg cttttcgccg ttacagattc
    17641 agctgcagtc gtgacgcgga aacagttgtt agcggccaga aagaccggag cgcagcgctg
    17701 gcaggtggag acggtgggtg ttcccatgac aatgttaccg ttcactgaga ttggcgatga
    17761 aggatactcc acatatctgc gggtgccata gcgagaatcg cggatgtttc cgttaaaggc
    17821 aacgacgacg actccttcgc tgcgctcgtg gcgataaagt ctctagcttg gcactatatc
    17881 gtgcaagcct caaaagaaag gccgcgcaat gcccgaacaa cctccccaga aaccaaacgt
    17941 aacagtggaa gatgaccgca ccggaacgag cgttacgacg ctgaaacagg ccatcctcga
    18001 caacctttat tacattgtgg cgaagacaac cgcaaatgcc acggaaagtg actacttcgc
    18061 cgccgttgcc tacaccgtcc gcgaccggat gttatcgcaa tggatagcca ctctggacag
    18121 ctattcgcgt gaagagttgc gcgtggtttg ttatctgtcg gctgaattcc tgatggggcc
    18181 gtacctcgcg aacaacatcc tgaacctggg attgaccggc ccttttcggc aggcgataga
    18241 agagctggga ctcgacttcg accgaatcat cgagcaggag agcgagccgg gcctggggaa
    18301 cggcggctta ggaaggctcg cggcgtgtta cctcgattcc ctcgccacgc tgggagtccc
    18361 tgcaatcggt tatggaatcc gctaccagtt cggaatgttt catcagcaga tccgcgacgg
    18421 ctggcaagtg gagatgactg actactggct gcggtatggc aatccatggg aaattcgccg
    18481 gccacaggcc tcggtggatg tgggctttgg tggccacacc gagccctaca cggacgcgaa
    18541 gggcaattac cgagttcgat ggatgcctcg ctcggtcgtc aagggaatcc cgtatgacac
    18601 gccgattgtg ggatatcgtt caggcgtggt caacgatctg cgactgtgga agtccgaagc
    18661 gacagagtcg ttttactttg agcgcttcaa cagtggcgac tatcagggcg cggttgcgga
    18721 gaagaacttc gcggaaaata ttacgaaggt cctctatcct aatgacgagg agatccaagg
    18781 caaagaactt cgattgcagc agcagtattt ctttacgtcg tgttccctgc aggacatgat
    18841 ccggttccaa caccgtatgg gctgtccgct ggagacgttt cataaagagt gggccattca
    18901 actcaatgat acgcacccgt cgattggcgt cgccgagctc atgcgattgc tgctggacga
    18961 gcacgacatg ggttgggacc aggcgtggtc gatcacgacc caaactttca gcttcaccaa
    19021 tcacaccttg ctgcccgagg cgctggaacg ctggccgctg ccgttattcg gttcgctgct
    19081 gccgcggcac ctggaaatca tctatgagat taatgcgcgg ttcttaacgg aagttcgcga
    19141 gcgttttccc ggcgatgaag cccgagtcgc acgcatgtcg atcatcgacg agcatgacca
    19201 gaagtacgtg cgcatggccc atcttgcctg cgtgggaagc cacaaagtta acggggttgc
    19261 ggaacttcat acgcaactgg tgaagcaaga actattccac gacttctacc agatgtggcc
    19321 ggagaagttc gtcaacgtta ccaacggggt cactccccgg cgttggctgg cggtaagcaa
    19381 tccggagcag tcggccctga tgaccagcaa gattggggat gggtggatca ggaatctcga
    19441 ggaattgaag cagctccaag gcttggctga tgattccgcc tttcgcgcgg aatggcgcaa
    19501 ggtccagcgg gacgttaagg tacggctgtc gaactacatt ctcaagtgca gcggtcagag
    19561 cgtcgacccg agttcaatgt tcgatgcgca ggtcaagcgc attcatgaat acaagcggca
    19621 gcatctcaac gtgcttcata tcattacgct ctacctgcgg ctaaagagca atcccaacct
    19681 ggagatcacg ccacgaacat ttctgtttgg tggcaaagct gcaccggggt acgtcatggc
    19741 caagctaatt atcaagttca tcaactcggt cggcgaggag gtcaacaatg acccggacgt
    19801 gaaacaacgg ctgaaggtgg tgtttctgcc cgactacgat gtgagcctag ggcagaaggt
    19861 atatccggcg gcggacctga gcgagcagat atccaccgcc ggcaaagaag cctctggtac
    19921 gggaaatatg aagttcgcta tgaatggagc gatcaccatc ggaactctgg acggagcgaa
    19981 tgtcgaaatc cgggaagcgg ttggcgcaga gaacttcttc ctgtttggtc tgaccgcttc
    20041 ccaggtttcg tccaccctcg ccgctgggta ccatccctgg gacatctacc aatcaaacgc
    20101 gggactccgt gaagcgatcg acagcgtggc ttcaggccag ttctctcacg gcgatcgcga
    20161 actcttccgg cctttggtcg attccctgct gcagcatgat ccctacctgc tgttcgcgga
    20221 ctatcaggct tatatcgact gtcaggatca agtcgcaagg gcattcggcg accaggagaa
    20281 ttggtcgcgc atggcgatcc tgaatgcgtt gcgaatgggc aagttctcgt ctgatcggtc
    20341 catcacggag tattgcgaag gcatttggaa cgtggcggtg aaagcatgac atcgggccta
    20401 cgcgagcagc caacccaaac aaagctgccg caaggctagt gtccggacag attggcagcg
    20461 atgtctccgg cgatggggac catgaagcgc tcaccctgat acgccttgat cacgcatacg
    20521 atccatagga cgaacatcac aagtccgatt aggctcagca gcattatccc aaaaccaaag
    20581 cctgcgaagt ggccgatgcc tccgaacggg ctgaatccac cgaacgccca gccaagtccg
    20641 aacattattg ccaccaccct gtggatgata ctcagcccgc caaaggtcac gatggattga
    20701 gcggcatgga agcgcacgta aggtcgcttg tcgatcaggt aaaagatgat gccggtcaac
    20761 cagccgagga cataactaag cgtggccgca gcgttttcgg aaaggccagc agtggctggc
    20821 tgataaggcg gcgacggtgg ccgaaaccca ggattgggcg ggggcgcaac ctggggttga
    20881 ccgcacttct gacagaactg cgcattatcc ggtaactcgg cgccgcactt ggtgcaatga
    20941 gccatacctg cctcctgctt cgtcactggc atgatatgcc agtttgcggt gggaggggta
    21001 tcaatctgca aatcgattac gcggctatca aaaatgaagg gcacggaatc gttccgtgcc
    21061 ctttgtgttg gcggaggaga ggcccctagg cgccgctgct tgcat
//
LOCUS       DASRKK010000024         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_98786, whole genome shotgun sequence.
ACCESSION   DASRKK010000024 DASRKK010000000
VERSION     DASRKK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98786"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..2903
                     /locus_tag="VFC15_00510"
     CDS             609..2903
                     /locus_tag="VFC15_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM08676.1"
                     /translation="MIDTTISHYRIVGKLGGGGMGVVYKAEDTRLGRFVALKFLPNEV
                     ARDPQALERFRREARAASALNHPNICTIYDIGEEDGRAFMVMEFLDGVTLKHMIEGRP
                     LEWEKLLAIAIEIADALDAAHSKGIVHRDIKPANIFVTEREHAKVLDFGLAKVAPTPG
                     SSSQGAWASAATAVEEQHLTSPGSTLGTVAYMSPEQVRGKDLDARSDLFSFGVVLYEM
                     ATGALPFRGESTGVIFDGIMNRTPAPALRLNPALPAEFERILEKSLEKDRGLRYQSAA
                     ELRTDLRRIQRASDSGRTASVDTPTPVHSPLKRGIVFAGLALLVLLAIAGVAWYEKHS
                     PAPSGTGAAKPSVAVLPLQNVSAEPDSAYFSQGMTDEITTKLSKIQGIDVASHSSVAA
                     LKGSDKSAAEIGKALGVRYLLEGNVQKAGDQVRINVHLIDSTTGFQVWADDFVGKMND
                     VFSLQEQTAMKIAGSLNLKLSPQEQQAIQHRYTQNPQAYDAYLRGQALVQYFDQPEQL
                     EAARRHFEEALQSDPDYPLALAGLSWVEGQIYRNLDSNPSHLQRAEQFAQRALAVDPR
                     LAETHLALGTVMADKYDYTGAAREFRTATDLDPDNAYAWDLLSWSLGYEQPPEAAAAE
                     KAVREALRLAPSMYEAYYHLGRALLAQQRYAEAITAFEHARELSPGASTSYLGLGQVY
                     LAQGDYQKAVAALSKDIRPAGINYFWLAAAYAAGGNTEKALATLQKAFDVGFHDFAAL
                     DTSPYFATLRRDPRFQQLMQRYRK"
     gene            complement(3284..3688)
                     /gene="ssb"
                     /locus_tag="VFC15_00515"
     CDS             complement(3284..3688)
                     /gene="ssb"
                     /locus_tag="VFC15_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZM08677.1"
                     /translation="MQKVMLIGNLGRDPDVKYSQQGTAIAQFSVATTERWKDKGGELQ
                     EHTEWFAVKTFGRRAEIVGENLHKGSRIYLEGRKRTESWDDKQTGAKHYRDLVYVDRI
                     EFLDSRTRENGHRDRAESASQETTVTDQETPF"
     gene            complement(3727..4362)
                     /locus_tag="VFC15_00520"
     CDS             complement(3727..4362)
                     /locus_tag="VFC15_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08678.1"
                     /translation="MAATDRITPIDESEVAVNESSGSECSEPTRAVRRHLPDERRALT
                     HHFSIAGQEGYLTVGVYEDGLPGEIFITMAKQGSTISGLMDAFATVVSLALQHGVPLR
                     VLCEKLSHMRFEPSGWSGNPKIGYAKSLMDYIARWLELRFLTGDQGELFKALPMPIQP
                     PTDANDSDPVGALGELIQMGDAPACNICGSLMVRSGTCYRCMTCGGTSGCS"
     gene            complement(4331..4576)
                     /locus_tag="VFC15_00525"
     CDS             complement(4331..4576)
                     /locus_tag="VFC15_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08679.1"
                     /translation="MHSKVKNYALVFCLFVFLFVTIGGDLHMNPGGAFLAAATGTPLM
                     IYAFNRRSSRDCRPEHVPSRLAGNEVRYGSDRSDNSD"
     gene            complement(4605..>4868)
                     /locus_tag="VFC15_00530"
     CDS             complement(4605..>4868)
                     /locus_tag="VFC15_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08680.1"
                     /translation="LETRLLFRGIHGRLDLDLSGKDKDKAGTALPTFYSRAGEEVRIP
                     KRFRQSVRAITAAVHCDGCTHRHYVRPPQKKEDDSCAAESVTV"
BASE COUNT         1005 a         1454 c         1365 g         1044 t
ORIGIN      
        1 gaggcaggag cggaaggtag gtggcaagtc caaatccggg ctgacattta ctgaaacgct
       61 caattcgatg cgaattgctc gtgcaggaac atggctggca cgccgacaac gcgtactcgg
      121 aaacatcgtc ccaagcatgt acgccccttt gctcgccaga acgagaaacc gaaaccttaa
      181 cgatcggctt ctcggacatg atgtgcaatg ttggaaacgc tgcaataaag gacgctatcg
      241 ccaacatcgc gcgcaatcgc gaggtcctac acttcttaag ttgcatcgcc cgttttctgg
      301 ggcacttctc cggtttggcg actatacgtc caagtggatc gcctgacgca agggcgttta
      361 tcgagaccaa tcaactgatg gcttctgatc agccaatgac agatacgtgg taggcagaga
      421 agccatcgcg cgcgcgctcc agcctcaact acgctttaat tcatgcgacg ccgattttgc
      481 gccaactggc agattggagc tttgcgttac ttccggttag ctacgatcag ggattcagtc
      541 aacaagaggc gcagcacgga ttgactgcga attacaatcg cccgtatcat cagccatcca
      601 gccccatcat gattgataca acgatctcgc actaccgtat cgtaggaaag ctcggtggcg
      661 gtggcatggg cgtggtgtac aaagccgagg atacgcgcct cggccggttt gttgccctca
      721 agttccttcc caatgaagtc gctcgcgacc cacaggcgct ggagcgcttc cgccgggagg
      781 cacgggccgc gtcggcgctc aaccatccca acatctgtac catctacgac attggcgaag
      841 aagacggccg cgccttcatg gtgatggagt ttctcgacgg cgtgaccctg aagcacatga
      901 tcgaggggcg tcccctggaa tgggaaaagc ttcttgccat cgcgattgag atcgctgacg
      961 ccctggacgc cgcccactcg aaagggatcg ttcaccgcga catcaagccc gccaatattt
     1021 tcgtcaccga gcgcgagcat gccaaggtcc ttgattttgg attggccaag gtcgcgccta
     1081 cacccggttc ttccagccaa ggggcatggg caagcgcggc caccgcggtt gaggaacaac
     1141 atctcaccag tccaggttcc actctcggga cagtcgcgta tatgtctcca gaacaagtga
     1201 gaggcaagga cctggacgcg cgctcagatt tgttctcatt cggagttgtc ttatatgaga
     1261 tggcaaccgg cgcgttgcct tttcgaggtg agagtaccgg ggtgattttt gacgggatca
     1321 tgaaccggac cccggctccg gcactgcgct tgaacccggc gctacccgcc gagttcgagc
     1381 gcatccttga aaaatcgttg gagaaagacc gaggactgcg ctatcagtcc gcggccgagt
     1441 tgcgcacgga cttgcggcgc atccaacggg cctcggactc ggggcggacc gcttccgtgg
     1501 acacgccgac cccggtgcat tcaccactca agcgcggaat cgtcttcgcc ggcctcgctc
     1561 tgctggtctt gctggcgatt gcaggggttg catggtacga gaaacattct ccggcaccat
     1621 cggggaccgg tgccgccaag ccatccgttg ccgtccttcc gctgcaaaac gtgagcgcgg
     1681 agccagacag cgcctatttc agccaaggaa tgaccgatga aatcaccacc aagctctcga
     1741 aaattcaggg aatcgacgtg gcttcgcatt cttcggtcgc tgcgctaaaa gggtctgaca
     1801 agagtgccgc ggagatcggc aaagcgctcg gcgtccgcta tctgctcgag ggaaatgtgc
     1861 agaaagcggg agatcaggtg cggattaacg tgcatctcat cgactccacc accgggttcc
     1921 aggtgtgggc cgatgacttc gttggaaaaa tgaacgacgt cttctccctg caagagcaaa
     1981 ccgcgatgaa gattgctggg tcgctcaacc tcaagctcag tccccaggag cagcaggcga
     2041 ttcagcatcg ttacacgcag aatcctcagg cctacgacgc ctacttgcga gggcaagcgt
     2101 tagtccagta ttttgaccag cccgaacaat tggaggcagc acggcgacac tttgaagagg
     2161 cgctgcagtc ggacccggat taccctcttg cccttgccgg gctgtcctgg gtcgaagggc
     2221 agatctaccg taatctcgat tccaatccgt ctcatctgca acgtgctgag cagttcgccc
     2281 agcgcgccct ggccgtggac ccgcgattgg ccgagaccca cctggcgctg ggaacggtca
     2341 tggccgacaa gtacgactat acgggcgcgg cgcgggagtt tcgcaccgct accgatttgg
     2401 atcccgacaa tgcctatgct tgggacctgc tttcttggtc actcgggtac gaacagccgc
     2461 cggaggccgc cgctgcggag aaggccgtac gggaagccct ccgtctggcg ccttccatgt
     2521 acgaagccta ctatcacctt ggtcgggccc ttctcgccca gcaacgctac gcggaggcta
     2581 ttaccgcctt cgagcacgcg cgggaattga gcccaggggc ttccacatca tacctggggc
     2641 tggggcaagt ctatctggcg caaggagact accaaaaagc tgtggccgcc ttatcgaagg
     2701 acattcggcc ggcgggcatc aactatttct ggctcgccgc ggcatacgcg gcaggcggga
     2761 acaccgagaa ggccctggcc acgctgcaaa aggcgtttga cgtgggattt cacgattttg
     2821 ccgccctcga taccagccca tatttcgcta ccctgcgcag ggacccgcgc ttccagcagt
     2881 tgatgcaacg ctaccgcaag taatgcgcgt agtgctgcgg cgctaacttt gaccggccgg
     2941 aaatcgcgca actggtctgt acgaaaaggg ccatccctcg ccaacattga tctgtcgtgg
     3001 tcgtgtcgcg ggctattcgg aggttgactc agctggcaag aacagggctt tgcgtgagtt
     3061 gaggcggccg ttaggacagg cgcggtcggt cctcgaacca tcccttcctt tcgattagta
     3121 atgataagtc ggccagggtt cacgaatgtg ttcacgaatg tgttcacgag tgtgttcacg
     3181 agtgtccgct gcagacgata cgatctggtt cgaatgtcac ttgatgagaa cagcgcttag
     3241 tacacgtaga ggaaccgatg gacgcaggaa tcgcgatcgt tcgtcaaaac ggcgtttctt
     3301 ggtcagtcac tgttgtctct tgagaggcgc tttccgcgcg atcccgatgg ccgttctcgc
     3361 gggtcctgga atccaggaac tcgatcctgt cgacgtagac gagatccctg tagtgcttgg
     3421 ctcccgtctg tttgtcgtcc caagactccg tacgcttccg gccttcgaga taaatgcggg
     3481 aacctttatg caggttctcg cctacgatct ctgcccgccg accaaaggtt ttcacagcaa
     3541 accactccgt gtgttcttgc agttcgccgc ccttgtcctt ccatcgttcc gtggtcgcga
     3601 cggagaactg cgcgatggct gttccttgtt ggctgtattt cacatcagga tcgcggccga
     3661 gattcccgat caacattact ttctgcatct tttgttcctc ctttctatct ctctcgtgct
     3721 tgcccgctac gaacacccgc ttgtacctcc gcacgtcatg cagcgatagc aggttccgct
     3781 ccgtaccatc agcgaaccgc agatgttgca ggcgggcgcg tctcccatct ggataagttc
     3841 tccgagcgct ccaaccggat cgctatcatt ggcatccgtc ggtggctgaa tgggcatcgg
     3901 aagggctttg aacaattcgc cctgatcacc cgtcaagaac ctcagttcga gccaccgcgc
     3961 gatgtaatcc atgagggatt tggcgtagcc gatcttcgga tttccagacc agccgctggg
     4021 ctcgaaccgc atgtggctga gtttttcgca aagcaccctc aatggcacgc cgtgctgcaa
     4081 ggcaagcgag actaccgttg cgaaggcatc catcagccca gagatggtcg agccctgctt
     4141 tgccatggtg atgaagattt ctcctggcag cccgtcttcg tagactccga ccgtgagata
     4201 tccctcctgc ccggcgatgg aaaagtgatg ggtgagtgcc cgccgctcgt ccggcaagtg
     4261 ccgccgaacc gcgcgcgtgg gttcggaaca ttctgaacct gagctctcgt tcaccgcgac
     4321 ttcactttcg tcaatcggag ttatccgatc tgtcgctgcc atatcgaacc tcatttcccg
     4381 caagccggga tggcacatgc tctggtcggc aatccctact ggacctgcga ttgaaggcat
     4441 aaatcatcag tggcgtgccg gtcgctgcgg caaggaatgc accgcccggg ttcatgtgca
     4501 ggtcgcctcc gatcgttacg aacaaaaaca caaacagaca gaaaaccagc gcgtagttct
     4561 tgaccttgct atgcatcgct attcactcct tccgttcagc catgtcagac tgtgacactt
     4621 tccgcggcgc acgaatcgtc ctctttcttc tgcggcggcc tgacatagtg gcgatgggta
     4681 cagccgtcgc aatgaaccgc cgcggttatc gcgcgcaccg attggcgaaa ccgcttcggt
     4741 atcctgactt cctccccggc gcgcgaatag aacgttggca gagccgtgcc agctttgtcc
     4801 ttgtcctttc cggacaaatc cagatccaag cgaccatgaa tgccccgaaa caggagtctt
     4861 gtttccag
//
LOCUS       DASRKK010000025         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_98844, whole genome shotgun sequence.
ACCESSION   DASRKK010000025 DASRKK010000000
VERSION     DASRKK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98844"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFC15_00535"
     CDS             complement(<1..202)
                     /locus_tag="VFC15_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08681.1"
                     /translation="MLKAASPLFAEQAQTIRQRLLGSTVIGSDETRFRVGKDNWWLWV
                     FQNADSCFFTIAPNRKKDVERRF"
     gene            307..1932
                     /locus_tag="VFC15_00540"
     CDS             307..1932
                     /locus_tag="VFC15_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08682.1"
                     /translation="MPRLDSVRFFADVPSPSNHSDLSRPEIEALLVEQFGEVAALKQT
                     VSELREEIARLKGLKGRPTIKPSKPSGMDNATEPLKLARKEKRRFRGKVTPRVNIEDQ
                     VVKIAVPEGSRFKGYEPFLVQDLVISAKATCYQRERWVTPDGRTILAPLPEGIDGHFG
                     PELRRFVLMQYHQGQSTLPRLAKFLRSVGVAISKRQLQRLLTDKQESFVAEAQDVLRA
                     GLETSPYVSVDDTGARHAGKNGFCTQIGNDWFTWFGTRTSKSRLNFLDLLRAGHTDYV
                     LNDAAYGYMRKQGLPAASIAHLMAQPETCFADQKTWQAHLDRLGFTALDVTPNPVQVA
                     TEGALWGSVQSHKFLCDAVVLSDDAGQFNVGQHALCWVHAERLVHKLDAFTDKHRAAQ
                     TRVRGLIWDYYADLKAYRLKPSPRRAAALRTRFDRIFLRRTGFVTLDRLLKRLHANKA
                     ELLMVLDRPETPLNTNGSENDIRCYVTRRKVSAGTRSDVGRDCRDAFLGLAKTCDKLG
                     IAVWDYLGSRLKVAGHIVIQPLDRYVRGRFRPA"
BASE COUNT          427 a          658 c          674 g          400 t
ORIGIN      
        1 gaaaccgcct ctccacatcc ttcttgcggt tcggggcaat ggtgaagaag cagctgtcgg
       61 cgttttggaa gacccacagc caccagttgt ctttcccgac acggaaacgc gtctcgtccg
      121 acccgatcac cgtgctcccc aacagacgct gacggatggt ttgcgcctgc tcagcgaaca
      181 gcggggatgc ggctttgagc acttaccctt tacccacatc tcagaactgc cgagtggaat
      241 cagtgggtta acatcaacga tggtgcgtaa tgggggcaaa aatgttggcg gccctacatt
      301 tttttcttgc cccggctcga ctcggtgcga ttctttgcgg atgtgccgtc gccatccaac
      361 cattccgacc tgagccgacc ggagattgag gctctgctgg ttgagcagtt tggcgaagtc
      421 gcggctctga aacagacggt cagcgaactg cgtgaagaga tcgcccgtct gaaaggcttg
      481 aagggccgcc ccacgatcaa accgagcaaa ccgagcggca tggacaacgc gaccgagccg
      541 ctgaagctgg ccagaaagga gaagcggcgg ttccgtggca aggtgacacc tcgggtcaac
      601 attgaagacc aggtggtaaa gatcgcggtc cccgaagggt cccgcttcaa gggctatgag
      661 ccgttcctgg tgcaggacct ggtgatttca gccaaggcca cctgctacca gcgcgagcgc
      721 tgggtgacac ccgatggacg cacgatcctg gcgccgctgc cggaggggat cgatggccat
      781 ttcggtcccg aactgcgccg cttcgtgctg atgcagtacc atcagggaca atcgacactt
      841 ccccggctgg cgaaattcct gcgctcggtg ggcgtggcga tctccaagcg acagctacag
      901 cgtctgctga ccgacaaaca agaaagcttc gttgccgaag cgcaggacgt cctgcgcgcc
      961 gggctggaga catcgccgta tgtgtcggtg gacgataccg gcgcgcgcca tgccgggaag
     1021 aacggcttct gcacgcagat cggcaatgac tggttcacct ggttcggcac ccgcacgtcg
     1081 aaaagccggc tgaattttct cgatctgctg cgcgccggcc ataccgatta tgtgctcaac
     1141 gacgcggcct acggctacat gcgaaagcaa ggcctgccgg cggcgtcgat cgcccacttg
     1201 atggcccaac cggagacatg cttcgccgat cagaaaacgt ggcaggcgca tctcgaccga
     1261 ctcggcttca cggcgctcga cgtcacgccc aatccggtgc aggtggccac cgagggagcc
     1321 ctgtggggca gcgttcagtc gcacaaattc ctctgcgacg cggtggttct gagcgatgac
     1381 gccgggcagt tcaacgtggg tcagcacgcg ctgtgctggg ttcatgccga acggctggtg
     1441 cataagctgg acgcgttcac cgacaagcat cgcgccgcgc agacccgggt gcgcggactg
     1501 atctgggact actacgcgga cctcaaggcc taccgactca agccaagtcc gcgccgcgct
     1561 gccgcattgc gcacgcggtt cgaccgcatc ttcctgcgcc gcaccggctt cgtcacgctg
     1621 gaccgtctgc tcaagcggct gcatgccaac aaggccgagt tgctgatggt gctggaccgg
     1681 cccgagacgc cgctgaacac caacgggtcg gagaacgaca tccggtgcta cgttacccga
     1741 cggaaggtca gcgccggaac ccgcagcgat gtcggccgcg attgtcgcga tgcgtttctg
     1801 ggtctcgcca agacctgcga caagctcggc atcgcggtct gggactatct gggcagcagg
     1861 ctcaaggtgg cggggcacat cgtgattcag ccgctcgacc gctatgtcag gggccggttc
     1921 cggcccgcct gactggcgga gccgccagca cttttgcccc tattacaaaa ggcggatgta
     1981 agccgttgat aggacgggtt ttaattttga gatgtcaaag atgggttaac gcgcgatcac
     2041 cttgtggtct gggtccgggc atggggtatt gtatcccgtc actgtgacgg gatacaatag
     2101 gatcgcgccc tggtgagaac caacaaccca cctcgtgacg tgacgggtta cccgctgga
//
LOCUS       DASRKK010000026         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_587434, whole genome shotgun sequence.
ACCESSION   DASRKK010000026 DASRKK010000000
VERSION     DASRKK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587434"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..821
                     /locus_tag="VFC15_00545"
     CDS             336..821
                     /locus_tag="VFC15_00545"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HZM08683.1"
                     /translation="MNLPECDLKTCARILAAALLFALPFPARAVLGDSAASVLADQAR
                     MKGTLRSVDNRTYVMHEITTTTGAVREFVSPGGAVFGVAWEGQFPPNFQQLLGPYYQQ
                     VQQAAAQQASEQPTRRRRGPVMIETPGIVFSQGGHMRSFHGQAYIPQLLPQGVQASDI
                     R"
     gene            complement(783..1160)
                     /locus_tag="VFC15_00550"
     CDS             complement(783..1160)
                     /locus_tag="VFC15_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08684.1"
                     /translation="MAIPDLLVSWVEVAVIVTWSDVTPPDGAVNKPEAEIVPALAAHV
                     TAELKLPVPVTVAEHWLVWPYWMVEGEQLTLTDVIVVGGTLLPLPPQAAISVRLASTS
                     NSPSFCTIVSPLADIAGLDALRQ"
     gene            1153..2337
                     /locus_tag="VFC15_00555"
     CDS             1153..2337
                     /locus_tag="VFC15_00555"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HZM08685.1"
                     /translation="MATVTVNPVQQTGNVQPIVVDAGPAPQSFVTANEAFTSVTVCLP
                     NSSTCQTIDHVLVDTGSSGLRLLSSAGGGELTLGLPQTNDSSGNPLYECEVFLDGYTW
                     GSVSAADITVASEKASSVPVQVVVPSSASPAVPASCSSQNPPGGTGNEGDSLMAFGAN
                     GILGIGLFQQDCGGFCVTNSTNPTVYYDCPSSGCTPTTATLLQQVPNPVAMFATDNNG
                     VLIQLPSVPDGGLASVQGSLIFGIGTESNNGLGSAQVYDVPDSGSDAGGFTTIFNGNT
                     YTSSFIDSGSNGFFFNDSSIPTCPSPNQDWFCPTTSPLSLSAQNQGTNMSSGVQVNFT
                     IENENNLSGSNTAFSTLAGPYGSTPPAEFDWGLSFFYGRNVFTAIDGMSTPGGTGPYF
                     AY"
     gene            complement(2462..2848)
                     /locus_tag="VFC15_00560"
     CDS             complement(2462..2848)
                     /locus_tag="VFC15_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZM08686.1"
                     /translation="MKLRNIALYLTLLLAVTALPLFAQSAASSGTAGGKATQTSKPMS
                     SATSPSEKIDINSATKDELGVLPGIGSTYSQKIIDGRPYNSKRDLLTRKIVPQKTYDG
                     IQDKIIAHRATDAKSKSSTGTPPKTQ"
BASE COUNT          597 a          862 c          845 g          608 t
ORIGIN      
        1 tgatattgcg gtcgatgatg taggtcacca cgccgtcggg gtgcaatctg cggcgcaccg
       61 cgtcggcttc catcccgatg ccaatcaggt catcggagcg atacatttcg agcgcttcgg
      121 ttttggtcag agacatagga cgtcacaacc cgcagtgaca ttctaaccgg gcagagaggg
      181 agcagggaag ccggccgtct atttgtcagc gggggaaact taaccaaagg ctggcacatc
      241 caaccctaac ggtgcctcgt gcgcaacaat cctgcgcaaa tggagtttat agcaacgggt
      301 tcctcaatgc gccgcacggc ggaggcggtg aaccaatgaa cttgcctgaa tgcgatctaa
      361 aaacatgtgc acgaatcttg gcggctgcgc tgcttttcgc gctgccgttt cccgcacgag
      421 ctgttctggg cgatagcgcc gcttcggttc tcgccgacca ggcccggatg aagggcacgc
      481 tgcgcagcgt ggacaatcgc acctatgtca tgcatgaaat caccacgacg acaggtgccg
      541 tgcgagagtt cgtctctcct ggcggagcgg ttttcggcgt cgcttgggaa ggccaattcc
      601 cccccaactt ccagcagttg ctcggcccgt actaccaaca ggtccagcaa gccgccgcac
      661 agcaggccag cgaacaaccg acccgccggc gccgcggtcc ggtcatgatc gaaacgcctg
      721 gcatcgtgtt ctctcaaggc ggacacatgc gcagctttca cggacaagcc tacattccac
      781 agctactgcc gcaaggcgtc caagccagcg atatccgcta aaggagacac tatcgtgcaa
      841 aaactagggc tgttgctcgt actggcaagc ctgacactga tggcggcctg cggcggaagt
      901 ggcagtagcg tgccgcccac gacgattaca tccgtgagcg tcagttgctc gccttcaacc
      961 atccaatatg gccaaaccag ccagtgctcg gcaacggtga cgggaacggg aagtttcagt
     1021 tcggccgtga cgtgggcggc gagcgccggc acgatctcgg cctcgggctt gttcaccgcg
     1081 ccgtcgggag gagttacgtc gctccaggtt acgattaccg cgacctcgac ccaggacacc
     1141 agtaaatcgg ggatggccac ggtaacggtg aatccggtgc agcagacggg aaacgtgcag
     1201 cccatcgtgg ttgacgcggg cccggctccg cagagctttg tcactgcaaa cgaggctttc
     1261 accagtgtca cggtgtgctt gcccaacagc agcacgtgcc agaccattga tcatgttctc
     1321 gtggacaccg ggtcttccgg cttgcgcttg ctgtcctctg ccggtggcgg cgagctgacc
     1381 cttgggctcc cccaaacgaa cgattccagc gggaatcctc tatacgaatg tgaagtattt
     1441 ctcgacggct atacgtgggg ttcagtgtcg gctgccgaca tcaccgtggc gagtgaaaaa
     1501 gcgtcctcgg ttcccgtgca ggtcgttgtc ccctcctcgg cctcacccgc cgtaccggcc
     1561 agctgcagca gccaaaatcc tccgggggga actggaaatg agggcgacag cctgatggcc
     1621 tttggcgcga atggaatcct tggcataggg ctctttcagc aggactgcgg aggattctgc
     1681 gtaactaact cgaccaatcc gactgtctat tatgattgtc cttcttccgg ctgtactccc
     1741 acgaccgcta ccctgttaca gcaggtgccg aatcccgtgg ccatgtttgc gaccgacaac
     1801 aatggggtgc tgatccaatt accatctgtt cccgacggcg gattagcatc ggtgcagggc
     1861 tcgctgatct ttggcatcgg caccgaatcg aacaacggcc taggcagcgc ccaggtttac
     1921 gacgtgccgg actccggcag cgatgctggc ggcttcacca ccatcttcaa tggaaacacc
     1981 tataccagca gcttcatcga cagcggctcg aacggatttt tcttcaatga ttccagcatc
     2041 ccaacctgtc ctagtcctaa tcaggactgg ttttgcccga ccacctcgcc cctcagtctc
     2101 agcgcccaaa accagggaac gaacatgagt tccggcgtgc aggtcaactt taccattgag
     2161 aacgagaata atctcagcgg aagcaataca gccttcagta cgctggctgg accgtatgga
     2221 agcacaccac cggcggaatt cgattggggg ctatcgttct tctatgggcg aaatgtgttc
     2281 acggctatcg acggcatgag tactcccggg ggcacgggac cgtactttgc gtattgacgg
     2341 aacgtggtcc catagagcgc gttccattac cggtatccaa gtcgtgacag ccggacagcg
     2401 ggccgcaagc ggccaacaag gagaaagggc ctgcggggta ccgttgtgag tttatccgct
     2461 ctcactgcgt ctttggaggc gtaccagtgc tgctcttact ctttgcgtcc gttgcccgat
     2521 gggcgatgat cttgtcctga ataccatcgt aggtcttctg cggaacaatc ttgcgggtca
     2581 gcaggtcgcg cttcgaattg tagggacggc catcgataat cttctgcgag taggtgctgc
     2641 caatgcccgg cagtacgcct agctcatctt tggtggcgct gttaatgtcg attttctcag
     2701 acggtgaagt ggcagaactc atcggcttgc tggtctgcgt ggctttgccg ccggccgtac
     2761 cgctggaggc tgcgctctgc gcgaataacg gcaaagcggt caccgcgagc agcaatgtca
     2821 gatagagtgc gatgttgcgg agcttcaagg ggacaccctc cttttgctat cagccgaatg
     2881 ctaattgttg gggagccgcc tgtcacgcag ca
//
LOCUS       DASRKK010000027         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1174043, whole genome shotgun sequence.
ACCESSION   DASRKK010000027 DASRKK010000000
VERSION     DASRKK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174043"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="VFC15_00565"
     CDS             complement(<1..2042)
                     /locus_tag="VFC15_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM08687.1"
                     /translation="MKLGPGDRLGSYEILSVLGAGGMGEVYRARDSALGRDVAIKVLP
                     DFVFTEPDRLVRFEHEAKAAAALNHPNIVGVYQFGTYEDSPYLVTELLEGETLRDRLG
                     HGPLPLRKTVDYGIQIAHGLAAAHKKGIVHRDLKPENLFLVKDGRVKILDFGVAKILQ
                     SDDKDAPGVDSAHLPTVATQPGVVLGTWGYMSPEQVRGQVVDQRSDIFALGAILHEMV
                     TGKRTFGRPTSADTISAILHDEPAAISQTVPRMPLGLQRVIDRCLEKNADQRFQSASD
                     MAFALEALSDTALTSTATRVQVKLQKARKRTALIVAALAALAGAFVLGYSLAHPSSRP
                     KASNYVQLTHDGQPKSLLGTDGARLFLGLGPFPYQGGAEMPTAGGEPRIIAMPSEHAV
                     PLVLSPDGSNLLAVDGQGVPPSGPLWAVPVTGGSPRRLGDLSGETGSWSPDGRMLAYA
                     KHNELFLANANGTESHKLCAVEGDINSIVWSPDGERLRLDASQTVGQHQFWEASTTGG
                     DQKRLLAGWHVPPDECCGRWTADGEYFIFQSNNQIWVLPAATYLNRSPSPIQLTSSPM
                     SVSTPIPSKDGKRLYVVGNVHRGELMRYDSATSQFLPFLAAISAEYAAFSKDGQWVTY
                     VTYPEGTLWRSKVDGGERMQLTYPPMYPMLPRWSPDGKTIMFFEFERAGKPSRIYE"
     gene            2458..>3314
                     /locus_tag="VFC15_00570"
     CDS             2458..>3314
                     /locus_tag="VFC15_00570"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM08688.1"
                     /translation="MELQTIGIDLGKTVFHLVGLNRCGEVVVRKKFSRKQLLHFTANL
                     QVELIGMEACGGAHFLGRALQEQGHAVRLMPAQYVKPYVKTNKSDYIDAEAIAEAVTR
                     PRMRFVPIKSDDQLDLQSLHRVRERWVMRRTAVVNQIRGLLLERGITIRKGRCHVDAA
                     LPGILEDASTKLSGALRLLLAQLRLELDHLQTRIDEADAVIQQTAGENEACRRLVAIP
                     GVGPVTATAIIAAIGNGAAFRKGREFSAWLGMVPGEYTTGGKQKLLGISKRGNAYLRR
                     LFVQGARAVL"
BASE COUNT          685 a          980 c          979 g          670 t
ORIGIN      
        1 atctcgtaga ttctcgatgg cttgcctgcg cgctcgaatt cgaaaaacat gatcgttttt
       61 ccatcgggag accaacgcgg cagcatcgga tacatcggcg gatacgtcag ctgcattcgc
      121 tcgccgccgt ccactttgct gcgccagagt gtcccttcgg gatacgtgac gtacgtcacc
      181 cactggccat ctttcgaaaa ggcggcatac tctgccgaga ttgcggcaag gaaaggcaag
      241 aattggctgg ttgccgagtc gtaccgcatc aattctccgc gatgtacatt gcccacaaca
      301 tacagcctct tgccgtcctt ggaaggtatc ggcgtagaca ccgacatcgg actcgaagtc
      361 aactgaatcg gcgagggcga gcgattcaga taggtcgccg ccggaagaac ccaaatctgg
      421 ttgttcgact ggaaaatgaa atactcacca tcggcggtcc atcgtccgca gcactcgtcc
      481 ggcgggacgt gccaccccgc cagcaaacgc ttttggtcgc ctcccgtcgt cgaggcttcc
      541 cagaactggt gttgacccac ggtttgcgat gcatccagcc gtaatcgctc gccgtcagga
      601 gaccacacga tcgaattgat atcaccctcc accgcgcaga gtttatggga ttctgtgccg
      661 ttggcattgg caaggaacag ctcgttgtgc ttcgcatagg cgagcattcg gccatctgga
      721 gaccacgagc cagtctcccc cgagaggtct cctagtctgc ggggcgaacc tcctgtgacc
      781 ggcacggccc acagcggtcc gctgggaggg acgccctgcc catcaactgc cagcaaattc
      841 gagccgtcgg gagacagcac cagcggaaca gcatgttcag acggcatcgc aatgatccgt
      901 ggctctccgc cggccgtggg catctcggct ccgccctgat aagggaaagg gcctaatccg
      961 aggaaaagcc tcgcgccatc cgtacctaac agcgacttgg gctggccgtc gtgcgtgagc
     1021 tgcacgtaat tgctcgcctt tggcctcgac gaggggtgtg ccagcgagta acccaaaacg
     1081 aacgcgcctg ccagtgccgc cagtgccgca acgatcagcg cggttcgttt gcgcgccttc
     1141 tgcagtttga cctgcacgcg cgttgcagtc gacgtcaggg ctgtatcgga caatgcctcc
     1201 aacgcaaagg ccatatccga cgctgattga aatcgctggt ccgcattctt ttccaggcag
     1261 cggtctatca ccctctgcaa ccccagcggc atcctcggca cggtttgcga aatggctgcc
     1321 ggctcatcgt ggaggatcgc actgatcgtg tcggccgagg ttggccggcc gaaggtacgc
     1381 ttgccggtca ccatctcgtg cagaatggct cccagcgcga aaatgtccga tcgctggtcc
     1441 acgacctgtc cgcgcacctg ctccggagac atgtagcccc acgtacccag cacgactccc
     1501 ggctgggtgg cgaccgtggg aaggtgcgcc gaatcgaccc ccggcgcatc tttgtcatcc
     1561 gattgcagga ttttcgcaac cccgaagtcg aggatcttga ctcttccatc tttgaccagg
     1621 aacaggttct cgggcttgag gtcgcgatga acaatcccct tcttgtgcgc ggcggccagg
     1681 ccatgggcaa tctggatccc gtaatccact gtctttcgca agggcaaagg gccgtgtccc
     1741 agccggtcgc gcagggtctc accttccagc aactcggtta cgaggtaagg gctatcctcg
     1801 taagttccaa actgatagac acctacgatg ttgggatggt tgagggccgc cgccgccttg
     1861 gcttcgtgtt cgaagcgcac caggcggtct ggctctgtga acacaaaatc aggcaagacc
     1921 ttgatggcga cgtcacgccc cagcgcactg tcgcgtgctc tgtagacttc tcccatgccg
     1981 ccggcgccaa gcacggagag aatttcgtac gatcctagtc tgtcaccagg tccgagtttc
     2041 aagggtgctc tgagtatacc ttcgagtttt caacttttat gcaatcaatt tctcgaagtc
     2101 gtttagctga gtctcagccc tcctgcagtg aagtgcctcg ccattaggag ttgtgatatc
     2161 cccatgtggt aactaatcct gagcaacagc cgatcggcga gtccgcctct ccgaagactg
     2221 ccgcgaaata ctcttctggg gagtttcatc acataggtaa cgagaatatc catggggccc
     2281 ggaatccact cggtcgtata gtcgccatgt cgcggaatga cagcgcgctc tggcggaggg
     2341 cgccagacgg aaccgtcggg cgtttccagt caagttgact ccgaacaggc caactaatgg
     2401 gatggtccgc cgccttgacc caagtcgggt atgagaggct gacgaaggag gcgtcccatg
     2461 gaactacaga caatcggcat tgatctcggc aagacagtct ttcatctggt tgggcttaac
     2521 cggtgcggcg aggttgtggt gcgcaagaag ttctcgcgca agcaactgct gcactttacg
     2581 gcgaacctgc aggtcgaatt gatcggtatg gaagcttgcg gaggagccca ttttcttggc
     2641 cgggcgctgc aagagcaagg gcatgcagtg cggctcatgc cggcgcagta tgtaaagccc
     2701 tacgtaaaga cgaacaagag cgactatatc gatgccgagg cgatcgctga agctgtgacc
     2761 aggccaagga tgcgtttcgt gccgatcaag agcgatgacc aactggacct gcagtccctg
     2821 catcgggtgc gcgagcgctg ggtaatgcgt cgtacggccg tagtcaacca gattcggggc
     2881 ttgctactgg aacgtgggat cactatacgc aagggacgtt gccatgtgga tgcggccttg
     2941 cccgggatac tggaagatgc cagcaccaag ctatcgggcg ccttgcgcct gctgctggcg
     3001 caactgaggc tggagctcga tcacctgcaa acccgaatcg acgaagccga tgcggtgatc
     3061 cagcagacgg ctggcgaaaa cgaagcctgc cgacgtttgg ttgcgatccc cggcgtcggg
     3121 cctgtgacag cgaccgccat catcgcggcg ataggtaacg gagcagcctt ccgcaagggt
     3181 cgggagttca gtgcctggct cgggatggtc ccgggagaat acaccaccgg gggtaagcaa
     3241 aaactgctgg ggatcagcaa acgggggaac gcctatttgc gaagactttt cgtgcaaggg
     3301 gctcgcgccg tcct
//
LOCUS       DASRKK010000028         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_685500, whole genome shotgun sequence.
ACCESSION   DASRKK010000028 DASRKK010000000
VERSION     DASRKK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685500"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1693
                     /locus_tag="VFC15_00575"
     CDS             113..1693
                     /locus_tag="VFC15_00575"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZM08689.1"
                     /translation="MPLSSKPNDDTSQAPPHHEPSTEVYAVHGADPEVLAYGMAKYSR
                     SALSMRESLAELNEQKAEKFLNTFYFQYGHRSIADLAHLAFAVERLSILAATVALDEP
                     RWDGQERSTRYQDFQKSGYFVPDFADDAGARALYRETVDLLFAEYRHFSDEMFRYLRD
                     RTPKPVEMKQDAYERTLRARAFDISRYLLPLATNTSLGQIVSARTLETQVSRLLSDTH
                     AEVRRLGELLKQAARDASYNVSHASARELVQEIQRECPAVAERASEFLLRDVRVAPTL
                     MKYAEPSPYEIETRRDLTAAAKELLASIAIQPAPLVDLLDDDPLEVELATTLLYQYSH
                     HSYRQIREVVEALPESQRREVIDLGLRHRGRYDEVSRAYCAGQQFRFDILMDIGGFRD
                     MHRHRRCVQIVQGYTAAHGYDVPPEIDSAGLAVRYDSVMERARDAAAELAARDVAGAE
                     EQSQYVIPLAFRKRTLFKMDLSEAIYISELRTGAAGHFSYRNVAYAMYEAVAQRYPAL
                     AKYFRVTDVREPVDLLKR"
     gene            complement(1970..>2908)
                     /locus_tag="VFC15_00580"
     CDS             complement(1970..>2908)
                     /locus_tag="VFC15_00580"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM08690.1"
                     /translation="KTVADRAVRAAAASYREHMAEFAEMKVLDTWYAQVSIDAIRDYF
                     RKDEDLSARLSKKVKQAHKQTSEAVIPKLTAVVDGLRKIKDNPPVIYHVHEFARNFEK
                     QRVKFTEEYKQSLQADRRHLYERYRYQDSAIKVVGVGSVGTRCYLSLLLADDDDPLFL
                     QIKEARRSVLESPRGKSRYAHQGFRVVEGQRVMQAASDIFLGWARTKGHDYYVRQFRD
                     MKVSAEIETFRPGTLVGYASLCGWTLARAHAKAGDAAMIAGYLGSSDQFDDALAQYSE
                     AYADQAERDFETFQAAIRSGRLSTEPAKGAGLEFLL"
BASE COUNT          561 a          894 c          836 g          617 t
ORIGIN      
        1 cctatcggca tgtcctcgcg gaaactgcag gtgggtcgcc gaggattttt tctgcgattc
       61 ccacatgcgg aacggttgcc catagattcc ttaccgcata tgattcacgg caatgccatt
      121 aagttccaaa cccaacgacg acactagcca ggcccccccc caccacgagc ccagcactga
      181 ggtctatgcg gttcacgggg ccgacccgga agtgctggcc tatggcatgg caaagtactc
      241 gcgctcagca ctttccatgc gggagtcgct ggccgaactg aatgagcaga aagccgaaaa
      301 gttcctcaac accttttatt ttcagtacgg tcatcgcagc atcgcggatt tggcgcacct
      361 ggcttttgcc gtcgagcgat tgtcgattct ggccgcgacc gtagccctcg acgaaccacg
      421 ctgggatggc caggaacgct ccacccgcta tcaggacttc cagaaaagcg gctactttgt
      481 tcccgatttt gcggacgatg ccggcgcacg cgccctctac cgcgagaccg ttgacctgct
      541 cttcgccgaa tatcgtcact tctccgacga gatgttccgc tatctgcggg accgcacacc
      601 caagccggtt gagatgaagc aagacgccta cgagcgcact ctgcgcgccc gcgctttcga
      661 catttcgcga tacctgctgc cgctggcgac gaacacttcg ctgggccaga tcgtcagcgc
      721 gcgcaccttg gagacgcagg tgtcgcgatt gttgtcggac acgcacgcgg aggtccggcg
      781 actgggagag ctgctgaagc aggctgctcg cgatgcttct tacaacgtga gccacgccag
      841 cgcgcgtgag ctggtacagg agatccagcg cgagtgtccg gcggttgccg agcgcgccag
      901 cgagtttctt ctgcgcgatg tccgggtcgc gccaacgttg atgaagtatg ccgagccgag
      961 cccctacgag atcgaaactc gtcgcgactt gacggcggcg gcgaaggagt tgctcgcatc
     1021 gattgccatc cagcccgcgc ctttggttga tctgctggac gacgatccgc tggaggtcga
     1081 gcttgccacg acgttgctgt accaatactc gcatcactcg tatcggcaga ttcgcgaggt
     1141 tgtagaggct cttccagaat cgcagcgccg cgaggtaatc gatcttggtc tgcgccatcg
     1201 cgggcgctat gatgaggttt cgcgcgccta ctgcgcgggt cagcaattcc gcttcgacat
     1261 tctgatggac atcggcggct tccgcgacat gcatcgccat cgccgctgcg tgcagattgt
     1321 gcaaggctat acggctgcgc atggctacga cgtaccgccg gagatcgatt cggctggcct
     1381 ggctgtccgc tacgattccg tgatggagcg tgcgcgtgat gccgctgctg agttggctgc
     1441 gcgcgatgtt gccggcgcag aggagcagtc gcaatacgtc atcccgctgg cctttcgcaa
     1501 gcgcacgctg ttcaagatgg acttgtcgga ggcgatctac atcagcgagc ttcgcaccgg
     1561 cgcggccggc cacttctcgt acagaaacgt cgcttatgcg atgtatgagg cggtggcgca
     1621 gcgttatccc gcactggcca aatacttccg cgtcaccgac gtgcgcgagc cagtggattt
     1681 actgaaacgc tgatccgtcg tcgcgatatt cccgcaacaa agctcgggcc gcagcgcgaa
     1741 gaggcgctca accagattac ttccgtgtag cccgtgacac gggaggaatc cggctcctga
     1801 gagttctcgc agggtagagc catttctccg ctcatgcgaa ggcgatttat caccaactga
     1861 gataggattg cggtccggat ggccgacaat acgggagcta tcgcgtcgca cctcgaatcg
     1921 ttgagcgatt ccctccggtt cccgtagcag gtcctagccc gcgtttccgc tatagcagga
     1981 actccagacc ggctcccttt gctggctccg tcgacaggcg cccggaacga atagccgcct
     2041 gaaaggtttc gaagtcacgt tccgcctggt cagcgtaggc ttccgaatat tgcgccaggg
     2101 cgtcgtcgaa ctgatcgctc gatcccaggt atccggcgat cattgccgca tcgccagcct
     2161 tggcatgggc ccgcgccagg gtccagccgc acaagctggc gtacccaacc agagtgccag
     2221 gccggaaggt ttcaatttcg gcggagacct tcatgtcacg gaactgacga acgtagtaat
     2281 cgtgtccctt ggtgcgggcc cagccgagaa agatgtcgct tgcagcttgc atgacgcgtt
     2341 ggccctccac cacgcggaaa ccctgatgcg catagcggct ctttccgcgc ggcgactcca
     2401 gcaccgacct gcgtgcttcc ttgatttgaa ggaagagtgg atcatcgtca tcggccagaa
     2461 gcagcgagag gtagcaccgc gtcccaacac tgcccacccc gacaactttt attgcactgt
     2521 cctgatagcg atagcgctcg tacagatgtc ttcgatccgc ctgcaacgat tgcttatatt
     2581 cctcggtgaa tttcacgcgt tgcttttcga aatttcgagc aaattcgtgg acatggtaga
     2641 tgaccggagg attgtcctta atcttccgaa gcccgtcgac aaccgcagtg agcttaggaa
     2701 ttaccgcctc agaggtttgc ttatgggcct gcttcacctt ctttgacaag cgggctgaca
     2761 ggtcctcatc tttccggaaa taatccctta tggcgtcgat gctgacttgc gcgtaccagg
     2821 tgtcgagcac tttcatttcg gcgaattctg ccatgtgctc ccgataagac gccgcagccg
     2881 ctcgcaccgc ccgatcagcg acagtctt
//
LOCUS       DASRKK010000029         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1439, whole genome shotgun sequence.
ACCESSION   DASRKK010000029 DASRKK010000000
VERSION     DASRKK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..343
                     /locus_tag="VFC15_00585"
     CDS             221..343
                     /locus_tag="VFC15_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08691.1"
                     /translation="MILAHSVSLGLKVVVDDGAQHNEAAWSRRLPDALLFLYGK"
     gene            complement(421..1332)
                     /locus_tag="VFC15_00590"
     CDS             complement(421..1332)
                     /locus_tag="VFC15_00590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZM08692.1"
                     /translation="MKNRKGIIAFLLIAFGLAWANWEIFFWLGFSMDNRLFKIASFPA
                     IFAPAIAAFIVRKWVTREGFADAGLRLNLRKWRYYVVAWLLPLVVVGCIVVLAPLLGL
                     GKADFSFGRGVSYFMQAGVPASRLRYPLLLIGGWLFGVFFAVPINFGEEFGWRGYLQL
                     RLFPDGPVLSAVVTGIIWAVWHYPLTLRGFNYADHPLIGSVLVFPVTGIFLSIIFRWL
                     NLKTGSIWSSSLAHSATNVIGGGVFILLFGGGNNSLFLSPAGMVAWIPLGALSAWIVF
                     TGQLKPANDGILRQAQIPALQADLQEL"
     gene            complement(1368..2180)
                     /locus_tag="VFC15_00595"
     CDS             complement(1368..2180)
                     /locus_tag="VFC15_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM08693.1"
                     /translation="MRFLQLVLAVFFLLGLPACRQLSAALSTQGSHDYQKGDYDRAIR
                     DYDNAIRWNPKSPGAFNGRGAAYVAKGNYDQAIQDFNEAIHLNPQSAGAFNGRGSAYF
                     AKGDYDRALQDLNEAIRLNPKSPRALLNLGIANIYAGHFSEAQQDLSQYLDPKDPYSV
                     VWLYLARAKGGTDGKDELKTNAAALNLSTWPGPVIQLYLGQNTPEDVLHAAGSDSGQR
                     CEYSFYVGEYRALRGERPEALALFRSARDECPKDFIEYVPAVTEVKDLEKQK"
     gene            2649..>3103
                     /gene="selD"
                     /locus_tag="VFC15_00600"
     CDS             2649..>3103
                     /gene="selD"
                     /locus_tag="VFC15_00600"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZM08694.1"
                     /translation="MLDSVLGKLARQHDPNVLIGFETADDAGVYQLTPELALVQTVDF
                     FTPIVDDPYVFGQIAATNALSDVYAMGGWPISALAMVCFPDSGDLKVLEEMLAGGLSK
                     MIEAHCALIGGHSIRDEEIKLGYAVTGTIHPQQVLANSGAKAGDRLIFT"
BASE COUNT          733 a          865 c          878 g          627 t
ORIGIN      
        1 atgaccgacc actacgtgga ggacctgcgg aagaaactaa tggcgggaaa gaagcccgag
       61 tcgtgatggt gggtttctcg gtgtacctcg gggctgaaaa gcatgcgtga gaactcgttc
      121 tgagttggca gggtagagcg atcgcgccaa atccagcccc gcagccgggg tcccgagttc
      181 gcgccgctct tgcgcgaaat ggggtggagg aggggcgaaa ttgattttag cccatagcgt
      241 cagccttggc ctgaaggtag ttgtggacga cggcgcacag cacaacgaag ctgcgtggtc
      301 gcgccgcttg ccggacgcgc tgctgttttt gtacggaaag taagaacaaa ggcaaccacg
      361 gatttcacgg atgaacaggg atcgaaatgg agccggtgtt ttgacggagg ttacatcgat
      421 ttatagctct tgaagatcgg cttgcaaagc tggaatttga gcttgtcgca aaatcccatc
      481 atttgccggc ttgagttggc cggtgaacac gatccacgcg cttaacgcgc ctaacggtat
      541 ccacgccacc attccagcag gcgagagaaa gagtgaattg tttccgccac cgaatagcag
      601 aatgaagacg ccgcccccga tcacgtttgt cgcggagtgg gcaagactcg atgaccaaat
      661 gctcccggtc ttcaaattta gccatctaaa aatgatcgaa aggaatatcc cagtgacggg
      721 gaagactagc acgctgccta tcagcggatg atcggcgtag ttaaacccac gaagagtcag
      781 gggatagtgc cagacggccc aaattatacc ggtaacgaca gcactcagga caggaccgtc
      841 ggggaatagc cgtagttgta gatagccacg ccagccgaat tcctcgccga agtttatcgg
      901 caccgcaaag aacacgccga acagccaccc cccaatgagc agcaaaggat acctcagtct
      961 gcttgctgga actccagctt gcataaagta gctgacccca cgcccaaatg agaaatctgc
     1021 tttaccaagt ccaagaagcg gcgcgaggac gacgatgcat cccaccacca ccagcggtaa
     1081 aagccaagcc acgacatagt atcgccactt ccgcaagttg agccgcagac cagcgtcggc
     1141 gaacccctcg cgagtaaccc atttccggac gataaaagca gcaatcgctg gcgcaaaaat
     1201 ggcaggaaat gaagcgatct taaataggcg gttatccatc gagaaaccga gccaaaaaaa
     1261 gatctcccaa ttggcccagg ctagcccgaa cgcaatcagc aaaaacgcga taatgccctt
     1321 ccgattcttc atgagatttc cgcctttcga ttgaggagtt gactcgctta cttttgtttc
     1381 tccaaatctt tcacttctgt gacggccgga acgtattcaa tgaagtcttt agggcactca
     1441 tcacgggcac tccgaaaaag agcaagagct tcggggcgtt cgcctcgcaa agcgcgatac
     1501 tctcccacgt agaagctgta ttcgcacctc tggccgctgt cactaccggc cgcatgcaag
     1561 acatcttctg gcgtgttttg accgagataa agctgaatca ccggaccggg ccatgtggac
     1621 agattcaagg ccgcagcgtt ggttttcagt tcgtctttcc catccgttcc ccccttggcg
     1681 cgcgccagat agagccacac cacgctgtag ggatctttcg ggtccaaata ctggctgaga
     1741 tcctgctgcg cttccgagaa gtggcccgca tagatatttg cgatgcctag gttaagaagg
     1801 gctcgcggag atttcggatt caggcgaatg gcctcgttca gatcctgaag ggcgcggtcg
     1861 taatcgcctt tggcgaagta agctgagcca cggccattga acgcacctgc agattgtgga
     1921 ttcaggtgaa tggcctcgtt gaaatcctga atggcttggt cgtagttccc tttggcgacg
     1981 taagccgcac cgcggccatt gaatgcaccc ggagattttg gattccagcg aatggcgttg
     2041 tcgtagtccc gaatggcccg atcatagtcg cctttctggt aatcgtggga accctgggtg
     2101 gatagggccg cggacaattg ccgacaggcg ggcaacccaa gcaggaaaaa caccgccagg
     2161 acaagctgca aaaatctcat tccgagcctc cccactgacc gcgccaagaa ttatcagatc
     2221 cacgataggt tgctctgcgg ctaacccgga tcggtaccgg agtcaggcat cacctgtgtg
     2281 ccttccgtcg gaattctcac tgtggctgca cactatatac ccatcgacgg ttccaccgcg
     2341 cggttctttt gcttggccgg gcgggcaatc gatctcttcc gtccgccgag tcagttcccg
     2401 gctgatcgcg gcatccgccg gcagcgctgc tgttctgtac ggcaagtaag agtcggcggt
     2461 tggcggttag cagttggcag ttggctcggc ggtttgacta agtgctaagt gctaaatgct
     2521 aagtactaac ggctaacagc gcccgtcgcg tgtggttgct agaatgaacc tgtatgtccg
     2581 acgtcaagac aacgcgcctc acggagatgg tgaaagccgc gggttgagcc agcaagctaa
     2641 gtccggcggt gctggactcg gtgcttggga aattagcccg gcaacacgat cccaatgtgc
     2701 tcatcggctt cgagacagcc gacgacgccg gcgtgtatca actcacgcct gagctggcgc
     2761 tcgtccagac agtcgacttc tttaccccca tcgttgacga tccctacgtt ttcggccaga
     2821 tcgcggccac caatgcgctc agcgacgtgt acgccatggg cggatggcca atcagcgcgc
     2881 tagcgatggt ctgcttcccc gacagcggcg acctcaaagt gctggaagag atgctcgccg
     2941 gtggactatc gaagatgatc gaggcccact gtgccttgat cggcgggcac agcattcgcg
     3001 atgaagagat caagctgggc tacgcagtga ccggaacgat ccatccgcag caggtgctgg
     3061 ccaacagcgg agcgaaggcg ggcgaccggc tcatcttcac caa
//
LOCUS       DASRKK010000030         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_978858, whole genome shotgun sequence.
ACCESSION   DASRKK010000030 DASRKK010000000
VERSION     DASRKK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978858"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFC15_00605"
     CDS             complement(<1..471)
                     /locus_tag="VFC15_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM08695.1"
                     /translation="MAKLPVEIPELVKQFGPTLNRVPAGGWVGSEKEDRMVKTHCCFC
                     GQQCGIQLRVRNNKVVGWEPWEDFPFNLGKLCPKGVKRYMQDEHPDRLKHPLQRVAGK
                     GFEPIGWDAALDRVVSEIQRIQQTYGNDAFALLTGASLTNEKAYLMGKFARVALR"
     gene            complement(475..1569)
                     /locus_tag="VFC15_00610"
     CDS             complement(475..1569)
                     /locus_tag="VFC15_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08696.1"
                     /translation="MDGLRPINLSPRGSDTAHSLVWGLIAAAALIVGIVVGSRGFKDF
                     DPALVSYAGATVFSAFGLGYRYAMWLRRPPTRLYWFRGWQIFLNPRTLPGNFARLALI
                     FWNNVVLQRFIERRSKTRWTAHWFLAWGCLIAAAVTFPLSFGWIRFETATDNQQIYRA
                     FVFGIHVASFPLDSIMAPLAFNILDICAVMVLIGIFVALWRRGRDRGAMSVQQFSNDM
                     LPLILLFAISVTGLFLTASTHLMRGLHYGFLSQLHAVTVIFTLLYLPFGKFFHIFQRP
                     AQLGIDFYKRAGEEGGQAQCRRCGQPFATKLQVSDLKAVQSALGIQYRMEDGTHYQDI
                     CPVCRRKNLALVQDALWRTPQAELERFERF"
     gene            complement(1573..>2289)
                     /locus_tag="VFC15_00615"
     CDS             complement(1573..>2289)
                     /locus_tag="VFC15_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM08697.1"
                     /translation="VLAAAIGFKWGFWTFGVLLSIWLVIFASMARNAPNRGPAKSFAE
                     IVRPLNDKRSWVLSLYYFLTFGGFVAMAVYLPTLLTEVFKLTPQDAGFRTAGFVVLAT
                     ALRPAGGVLADKVGGRAILKWVFPATALFAIFLACPLMSTFTVGALGMAASIGLGNGA
                     VFKLVPEYFPKSVGGVTGLVGAAGGLGGFFPPLVLGVIKQQTGTFTIGFILLGLFALI
                     CLLVLLRGSERRRLAVAANG"
BASE COUNT          517 a          693 c          694 g          385 t
ORIGIN      
        1 gcgcagcgcg acgcgcgcga acttgcccat caggtaggct ttctcgttgg tcagggaagc
       61 gcccgtcagc agtgcgaatg catcgtttcc ataagtctgc tgaatgcgct ggatctcgct
      121 gactacgcgg tctagcgccg cgtcccaacc gatcggctcg aagcccttgc cggcgacgcg
      181 ctgcagcggg tgcttcaggc ggtcggggtg ctcgtcctgc atgtagcgct tcacgccctt
      241 gggacacagc tttcccaggt tgaagggaaa gtcctcccaa ggttcccatc ccactacctt
      301 gttgttacgg actcgcaact gaataccaca ctgctgtccg cagaaacagc aatgggtctt
      361 caccatccgg tcttcttttt ccgaaccgac ccatccgccc gcggggacgc gattgagcgt
      421 gggaccgaac tgctttacta attccgggat ttcaacggga agttttgcca tagatcaaaa
      481 ccgttcgaat cgctccaact cagcctgggg agtccgccac agcgcatctt gcacgagcgc
      541 caggttcttg cgccggcata ccggacatat gtcctgatag tgcgtaccgt cttccatccg
      601 atactggatg ccgagggcgc tctgcacggc tttcaggtcg ctgacctgca gctttgtggc
      661 gaagggctgt ccgcagcgtc tgcactgcgc ctgcccgccc tcttcgccgg cgcgcttata
      721 gaagtcgatg cccaactgtg ccggccgctg aaagatgtgg aagaacttgc cgaagggtag
      781 atacagcagc gtgaagatca cggtgacggc atgaagctgg gagagaaagc catagtgcaa
      841 accgcgcatg agatgcgtgg acgcggtcag aaacaggccg gtcacggaaa tcgcgaacag
      901 caggatgagt ggcagcatgt cattggagaa ctgctgcacg ctcatggcgc cgcgatcgcg
      961 accgcgccgc cacagcgcca cgaagatccc gatcagcacc atgaccgcgc agatgtcgag
     1021 aatgttgaaa gccagcggcg ccattatgct gtccaagggg aagctggcca cgtggatgcc
     1081 gaacacgaag gcacgataga tctgctggtt gtcggtcgca gtctcgaagc gtatccatcc
     1141 aaaggacagc gggaaggtga ctgcggcagc gatcaggcat ccccacgcca ggaaccagtg
     1201 ggccgtccag cgggtcttcg aacggcgctc gatgaagcgc tgcagaacga cgttgttcca
     1261 gaagattagg gcgagccgcg cgaaatttcc cggcagcgtc ctgggattga ggaagatctg
     1321 ccatccgcga aaccagtaca ggcgagtagg cggacgccgc agccacatgg cgtagcggta
     1381 gcccaggccg aaggcggaga agacagtcgc acccgcgtag gaaaccaggg ccggatcgaa
     1441 gtccttgaag ccgcggctgc caaccacaat gcccacgatg agagcggcgg ctgcaatcag
     1501 gccccaaacc agggaatgtg ccgtatcgga gccgcggggc gaaagattaa ttggcctcag
     1561 accatccata actcaaccgt tggccgccac ggccagccta cgccgttctg aaccacgcag
     1621 caaaaccagc aggcagatca gcgcgaacag tcccagcaaa atgaagccta tggtgaaggt
     1681 ccccgtctgt tgtttgatga ccccgagcac cagcggagga aagaatccgc ctagtccgcc
     1741 ggctgcgccg accaatcccg tgacgccgcc cacggatttg ggaaagtatt cggggacaag
     1801 tttgaatacc gctccgtttc ctaaaccgat ggaagccgcc atgccgagcg caccgacggt
     1861 aaaagttgac atcaacggac acgccaggaa aattgcaaac agcgcggtgg ctggaaagac
     1921 ccacttcagg atcgcgcgtc cgccaacttt gtcggcgagc acgccgccgg ctggacgcag
     1981 cgcggtggcg agcacgacga agccggccgt gcgaaagccc gcatcttgcg gggtaagctt
     2041 gaatacctcg gtgagaagcg ttggcaggta gaccgccatg gcaacgaacc cgccgaaggt
     2101 caggaagtag tacaggctca gcacccagct gcgcttgtcg ttcagcggac gcacgatctc
     2161 ggcaaacgat tttgccggtc cgcgattggg agcattacgc gccatgctgg cgaagatcac
     2221 cagccagatc gaaagcagca cgccgaaggt ccagaagccc cacttgaacc cgatggctgc
     2281 ggccagcac
//
LOCUS       DASRKK010000031         4816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1369862, whole genome shotgun sequence.
ACCESSION   DASRKK010000031 DASRKK010000000
VERSION     DASRKK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369862"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFC15_00620"
     CDS             <1..312
                     /locus_tag="VFC15_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HZM08698.1"
                     /translation="ENGAGTNPEELIAAAHAGCFSMALSGQLGEAGMRAESIDTKATL
                     TMEETDAGFTITTVHLDVSVKIPGGDQAKFNQAAQNAKKGCPVSRVLNANITVDAKLI
                     S"
     gene            complement(1221..1619)
                     /locus_tag="VFC15_00625"
     CDS             complement(1221..1619)
                     /locus_tag="VFC15_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08699.1"
                     /translation="MFQQHLRRVGGGNARWVAHLSFLAQIPVPFEYHGRYSLPTLYGP
                     EGLFQIVTTALAAIEPGSLSFNLEIHPTEGRLALRDAAHLFQHWQVRSNAERMNYWLS
                     ILAENRQLLTAGLRTNSSHRNSGGKPGCPC"
     gene            1605..2354
                     /gene="istB"
                     /locus_tag="VFC15_00630"
     CDS             1605..2354
                     /gene="istB"
                     /locus_tag="VFC15_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM08700.1"
                     /translation="MLLEHHLKQLRLPTFLGEYDKVARQCAAESVDYPRYLLRLTEQE
                     LLDRERRATERRIHQARFPVVKSLDTFEFLAIPAVNKALVLELVRCEFLLRRENVLLL
                     GNSGTGKTHLALALGLSACQRGHRVRFTTAAAMVNELLEAKDEKRLLRFQKQMAGYEL
                     LIVDELGFVPLSKSGAELLFEVFSQRYERGSTLVSSNLPFQEWAEVLESERLTGALLD
                     RLTHHVHILEMNGESYRLKQSKRKRGSPPNS"
     gene            3436..4239
                     /locus_tag="VFC15_00635"
     CDS             3436..4239
                     /locus_tag="VFC15_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM08701.1"
                     /translation="MAVGASEQELIALNPLPPALRDMGFDREVAMNSPYGMELVEDCV
                     SCKLCSEGFFCHLPQSVMEAFQPLKFTIAYPAGATLFLEGQTCRGIYILCKGRVKLSA
                     TSREGQILILKVAQPGEVLGLNPTISGMPHETTAETGQPCQLNFIKRDDFLKFLKEHG
                     DACLHATILLSRECQQAYQQLRSFTMNSAPERIARLMLDWSHDDSGMATAHGIKVALT
                     HDEIGQIIGMSRETVTRTLANFRKQRIAEIHGSTLLIQNMSAIQKLAGA"
     gene            4407..>4816
                     /locus_tag="VFC15_00640"
     CDS             4407..>4816
                     /locus_tag="VFC15_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HZM08702.1"
                     /translation="MRSGNSCFDSVCIKSTGSYVPSKILTNEEVLANLPTSPEWVAEK
                     MGIRERHIAEPDEYTSDLAARAGLDAIASAGLEPNEIDLIIVSTATPDRKMPSSACIA
                     QAKMGIRNRCAAFDVAAVCAGFVYGITIAGQFIQ"
BASE COUNT         1042 a         1378 c         1383 g         1013 t
ORIGIN      
        1 gaaaatggcg ccggtacaaa tcctgaagaa ctgattgcgg cggcgcacgc cggttgtttt
       61 tccatggctc tttctgggca gctgggagaa gccggcatga gggctgaaag catcgacacc
      121 aaagcgaccc tgacgatgga agaaactgat gccggcttca ccatcacgac cgtgcatctt
      181 gacgtcagcg taaagatccc gggcggcgat caggcgaaat tcaaccaagc cgcacagaat
      241 gccaagaaag ggtgccctgt ctcacgcgtc ctgaacgcca atatcaccgt ggacgcgaaa
      301 ctcatttctt gatcccctcc catggctgcc cggctcaaac tgggcagcca acattatggt
      361 tcagcctgat ttctcgaggc agcccctgcc aagccgaact ctggtggcgt ggatgaaaca
      421 gtttgagttg agcgaccctc tgtcggttca gtgaccttcg catcagtacc tcctggagat
      481 agcaagcaac gatgctccgc ttgtagcaca acgtcacttt caggaagcag cgtgcattga
      541 agcagcgact tcagatccag caatgctttg caactgactg tgggcaccgc cgccaggcgc
      601 attcggcagc aggtcgccgg atcgaacatc cattgcggga tctccaggag tcgaacttcg
      661 aggttctctt cgatgctgca gcggaacaag gcgcgcccat tccgggtgaa ggattgatgg
      721 atcgccacaa cgcggtcgtg ccaaggatgc caggggtaac ggacttctcg cgattcaata
      781 ctatggatgt tgcgttgtcg acttgtacaa cgaagaccgg actcatctcg gactggggaa
      841 ggacacgccc attgggcgag ttgcgacatc ggtgttgcca ggtgggaaca aggtcatctc
      901 cttgccgcga cttggtgggc tgaatcaccg ctacatggta gcggcctgag ttcacaaccg
      961 cccgtgaatc accttcacgg tctaccttgg tgttggagcc cagggagaat cgcatctcca
     1021 atccgattca gacaggccca atcgctgccg aaacctcacg cgactctgcc cagacgtgaa
     1081 ttgacagcgc gcgcgctatc acaacgatca gcagccgaaa atggtggcgt ttttaatttt
     1141 ggcgacgcac actaccatcc gaacgcatgc cggtcttggg attcggtgcg tgggctgcta
     1201 ttaggagtcg gatggatttc ttagcaagga caaccgggtt tcccgccgga attccggtga
     1261 gaagagttcg tccgcagtcc cgccgtcagc agttgacgat tttcggccag aatcgagagc
     1321 cagtagttca tgcgctcggc attgctgcgg acttgccaat gttgaaagag gtgcgcggca
     1381 tcgcgcaggg caagccttcc ttccgtggga tgaatctcca ggttaaacga cagcgatccc
     1441 ggctcgatcg ccgccagagc cgtggtcacg atctggaaaa gaccctcggg gccatacagc
     1501 gtcggcagcg aatagcgtcc gtggtattcg aacggaacgg gaatctgtgc gagaaagctc
     1561 aggtgagcga cccatcgagc gttgccccca ccgacacgcc gcaggtgctg ctggaacatc
     1621 acctgaagca gctgcggctg ccgactttcc tgggcgagta cgacaaagtg gcgcggcagt
     1681 gcgccgccga gagcgtggac tatccgcgct acctgctgcg tttgacggaa caggaactgc
     1741 tggaccgcga gcgtcgggca accgagcgcc gcatccatca ggctcgcttt ccggtggtca
     1801 agagcctgga caccttcgag ttcctcgcca ttccggcggt caacaaggcg ctggtgctgg
     1861 agctggtgcg ctgcgagttt ctgctgcggc gcgagaacgt gttgttgctg ggcaactccg
     1921 gcaccggaaa aacgcacctc gcgctggccc tcggactgtc ggcctgccaa cgcggccatc
     1981 gcgtgcgttt cacgaccgcg gcggcgatgg tgaatgaact gctggaggcg aaggacgaga
     2041 agcggctgct gcgcttccag aagcagatgg ccggctacga actgctgatc gtcgatgaac
     2101 tgggattcgt gccgctgtcg aagtccggcg ccgaactgct attcgaggtc ttcagccaac
     2161 gctacgaacg cggttcaacc ctggtgagca gcaacctgcc gtttcaggag tgggctgaag
     2221 ttctcgaatc ggaacggctg accggcgcgc tgctggaccg gctcacccac catgttcata
     2281 tcctggagat gaacggcgag agctaccgcc tgaagcagag caaacgcaag cgaggctcac
     2341 cgccgaactc ctgatccgcc aaccgcccgc ctcaactccg cccgctaacc gctgcggcga
     2401 acgcgcctcg gggaggagga cgtcttccgc tccggcctaa acgcgccgcc ttgcaggcgg
     2461 cgcaggcctc cgctccagac gccctcctcc ccgacaattc ttctacatac tgtactggtc
     2521 tacttttgct ccgccccagt ggtctagttt tactacgccc ttgacatagt gaattggcct
     2581 gttctcaaaa gttatcttga taatcttgat agtatagatt ttacacgtta ccgctagcag
     2641 cccgtggtga aagtgatgtc attcagaaaa cagcgagtaa gacataaatc gcacccaatg
     2701 aatacaggca cttacgaccg acgctgcggc ctagcttgcg ggttcgagag gttcaccaca
     2761 ggctgctagg agggatattt gatgtccgtc gaatcgtatt gctaatcaat ccgccggggg
     2821 ctactatgcg acttagtggt gtggggaggt cgctcgcagt tgcaccaggc tgtaacggtg
     2881 gcgggtgacg atccgtgccc ccttcgactg cgcattttct ggtctctggt ttccctgact
     2941 gctctaagcg ataccggccc accgtgttca taccgctcat aaaaccggag gaccggggtt
     3001 atctgatttc ggctggttgt tcatgcgatc gagctgctaa ggaggtttct gtggtagcca
     3061 gatgctcaaa tcccgcatgt aatcgcgagt ttcgcgatct tcgtaagggc cgactcttcc
     3121 tgcttccccc gacgtatgtc tactcggatt cgatgaagct gtccgattat tgttactggc
     3181 tctgcccgga gtgcgacgca acacatacga tcatgcgacg cgagtcggaa gtggtggtca
     3241 gtgaacaagg acccggcact ccatatctca tcccggcctc ctcacatcgc aggaagcgac
     3301 ccgggcagat caccctgcgg ggtgatacgc aagtggggtg atttttcagt tgaactgcgt
     3361 ggcctcgcga atttcaacgc tatggccatg cgaggtggcg gagttggaaa gccctaaccc
     3421 agcacagcag aatccttggc agtcggcgcg tcagagcaag agttgatcgc cctgaatcca
     3481 ctgccaccgg ctctgcgcga tatggggttc gacagggagg tggcgatgaa ttcaccatac
     3541 gggatggagc tggtcgaaga ctgtgtctcc tgcaagttgt gttccgaggg ctttttctgc
     3601 catttgccgc aatcagtaat ggaagccttc caacccctga aattcacgat tgcgtatcct
     3661 gcgggcgcaa ctctgttcct ggaagggcaa acatgccgcg gtatttatat tctttgtaaa
     3721 ggccgggtga aattatcagc cacttcgagg gaaggccaga ttctcatatt aaaggttgct
     3781 cagcccgggg aggtgttagg gttgaaccca accatttctg gaatgccgca cgagaccact
     3841 gcggaaactg gccaaccgtg ccaactaaac tttattaaac gagacgactt tcttaagttt
     3901 ctgaaggaac acggcgatgc ctgcttgcac gcgacaatac tgctgagccg cgagtgccaa
     3961 caggcctacc agcagcttcg ctcgttcaca atgaactcag cacccgagcg aattgcccgc
     4021 ttgatgctgg attggtcgca cgacgattcc gggatggcca cggctcacgg aatcaaggtg
     4081 gccttgaccc atgatgagat cgggcaaatc atcggcatgt cccgcgagac agttacccgt
     4141 accctcgcca attttcgcaa gcagcgcatt gcggaaattc atggttccac cctgctgatc
     4201 cagaatatgt cagccattca gaagctggcc ggtgcataga ccgacaaccg cacacgcccg
     4261 gatgttttct cgggcagatt aggaacggcc atgaactcgg tttcatcatc taccgctgcg
     4321 aagcctccgc tcatattgtg tattgacgac gctgacatcg cgctgcgtgt aagaaagctc
     4381 ctctgttcgc gagactggag ggaggaatga gaagcggaaa cagctgcttt gatagtgtct
     4441 gcatcaaatc caccggttct tacgttccct ccaagattct gaccaacgaa gaagtactgg
     4501 cgaacttgcc aacctctccg gagtgggtcg ccgagaagat gggaattcgc gagcggcaca
     4561 tcgctgaacc agacgaatac acgtcggatc ttgctgcccg tgccgggctg gacgcaatcg
     4621 cttcggcggg gcttgagcca aacgaaattg acttgattat cgtttccacg gcgacacccg
     4681 accgcaagat gccctcatcg gcatgcatcg cgcaggccaa gatgggcatt cgcaaccgct
     4741 gcgccgcgtt cgatgtggcg gcggtgtgtg ccgggtttgt ttacggcatc acgatcgccg
     4801 gccaattcat ccagaa
//
LOCUS       DASRKK010000032         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_99382, whole genome shotgun sequence.
ACCESSION   DASRKK010000032 DASRKK010000000
VERSION     DASRKK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99382"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..520
                     /locus_tag="VFC15_00645"
                     /pseudo
     CDS             1..520
                     /locus_tag="VFC15_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(523..1593)
                     /locus_tag="VFC15_00650"
     CDS             complement(523..1593)
                     /locus_tag="VFC15_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HZM08703.1"
                     /translation="MLEHLVQAVGDIKEYRTGQNIHHPLHAMMVIIFLAVACGETSMK
                     GITRFVAESWREIGRILGLKSPPCRTTLRKAINLSGIVFGNDICGRHVRIDGKILRGT
                     RVGGQQVLLVEAWDGERLIGFESDSPGNEKAAARRVIEALDLAGKVVTLDAGLSDNNI
                     LSAVVERGGSYVARVKRNKPLLCDSIEQAFVGVPFQATDTDLAHGRIEVRESTVVDDP
                     ETIERIRSVHRVPKLAAVGRTDRVRISKKTGKTEMDACWYVIGPTVPKEEYGRYVREH
                     WEIEAMHCRADVTLNEDRCHSRTTETAVSLAVLRRLGMTVRRLLHPSQDHATALFKCR
                     FNPFRTFERLFNGAKNPAFQPK"
BASE COUNT          467 a          628 c          501 g          429 t
ORIGIN      
        1 ctactgcgcc gacgacgtgt cgctgtatga gaaattcctc atccggccgg tcggccaggt
       61 caacctgcca gccatcgcga atgaaaacgc caatatcgat cagatcgtcg cgacattggg
      121 cctcaaggaa atgacgcagg gcaccctgat caggaagctg tgcacctaca cccagccaaa
      181 cccgacgcgg cgcgcgatct tcgagttcga caagctcgtc cgcagcatct atactctccg
      241 ctacctgcgc gacccgcaac tcgagcgcac cgtgcaccgc tcgcaaaacc gcgtcgagtc
      301 gtaccaccag ctccgctcgg ctatcgccca ggtcggcggc aagaaggaac tcaccggcaa
      361 gaccgacatc gagatcgaga tcagcaacca gtgcgcgaga ctgatcgcga acgcgatcat
      421 ctactacaac tctgccatcc tgtcgcgatt gctggccagg tacgggacga gcccgagtgt
      481 gaaagctctc gccatgatca agaaggtctc acccagggcg tgtcactttg gctgaaatgc
      541 tgggtttttt gcgccattga agagcctttc gaaggtccgg aaagggttga accggcattt
      601 gaagagcgcg gtggcatgat cttgggatgg atggagcaac ctgcggacag tcatgccgag
      661 ccgccgcagc acggccagcg aaaccgcagt ctccgtagtg cgggaatggc agcggtcctc
      721 gtttaaagta acatcggcac ggcaatgcat cgcctctatt tcccaatgct ctcggacgta
      781 acgtccatac tcttctttcg gtactgttgg cccaataaca taccaacaag catccatctc
      841 agtctttccg gtcttcttcg atattcttac cctatccgtc cgtccaaccg ccgcaagttt
      901 tggaactctg tgaacggacc ttatccgttc gatggtttcc gggtcgtcaa ccaccgtcga
      961 ctcccgaacc tcgatccttc catgcgccag atcggtgtcc gtcgcctgaa acggcacacc
     1021 gacgaaggct tgctcgatgg aatcgcacag caacggcttg ttgcgcttta cccgagcgac
     1081 atacgaaccg ccgcgttcca ccactgccga caagatgtta ttatcggaca aacctgcgtc
     1141 tagcgtcacg actttacccg ctaaatccaa tgcctcaatc acccgacgcg ctgccgcttt
     1201 ctcgttacca gggctatcag attcaaaacc aatcagacgt tctccgtccc atgcctcgac
     1261 aagcaagacc tgctggccgc cgacccgtgt gccacgcaaa atcttcccgt caatccggac
     1321 atgtcggccg caaatatcgt tgccaaaaac gatgccggac aagttaatcg cctttcgaag
     1381 tgttgtcctg catggcggag atttaagccc aagtatcctg ccaatctccc gccacgactc
     1441 cgcgacaaaa cgagtgatgc ccttcatgct cgtctcgccg cacgcgaccg ccagaaaaat
     1501 aatcaccatc atggcatgca gtgggtgatg gatgttctgg cccgtcctat actctttaat
     1561 gtcgccgacc gcctgaacaa gatgttccaa catctcctac ctcccttgtg tgttccaact
     1621 tcaaggaaag cggtcggcgg caacataaat caagaaagtc aagagtttta tcgctgctga
     1681 atgttctgtt ctgttgacag atcggcctac agcctcaaag tgacacgccc tggggggtga
     1741 ggcacgttac aagcggagtc catggtgatc gatcagactg gccaacgaga cgtgggtccc
     1801 cacgagatca gccacattgc aacttgtccg tcctgatcca atggatagcc aagtatcaca
     1861 cccttgttga ctcacatgca ggaaatttga tagattggtt aaccgaccgt cccaagcggt
     1921 gctggccaat gccttgctga catccaatac atctccgtta cagaccgacc agttcgcgat
     1981 gcgatccagt ccgcgtgcgt tgagggtaac ggtgttgtcc gggcc
//
LOCUS       DASRKK010000033         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_99396, whole genome shotgun sequence.
ACCESSION   DASRKK010000033 DASRKK010000000
VERSION     DASRKK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99396"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1168)
                     /locus_tag="VFC15_00655"
     CDS             complement(233..1168)
                     /locus_tag="VFC15_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HZM08704.1"
                     /translation="MHEDYPKTLLELERRFCTEEACVEYLAALRWPGGWACPRCAGAD
                     AWAIRRTRWLCGHCRYEMSVTAGTIFQDSHVPLTIWFRAMWQITSQKNGMSALGLQRV
                     LGLGSYKTAWAMLHKLRRAMVRPGRDRLDGVVEVDEAYWGGEETGAIGRGAEEKALII
                     VAAEEDGKGIGRIRLRTIPDASSASLHGFIREAIAPGSTVRSDGWRGYLGLEGYVHDR
                     QVQSHQEDGGHLLPRVHRVIALLKRWLLGTHQGAIGLQHLDDYLDEFTFRFNRRTSKS
                     RGKLFYRLAQQAVQVGPIPFAALVKPQPVASGGVK"
     gene            complement(1137..2321)
                     /locus_tag="VFC15_00660"
     CDS             complement(1137..2321)
                     /locus_tag="VFC15_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZM08705.1"
                     /translation="MSERSTEQIRELLDSLYRVDSGRILATLIRLLGDFDLAEEAMHE
                     AFAAALSLWPRSGVPGNPRPWLISTARFKAIDTLRRRARFEASRDELVRYLEAQWSSA
                     ERSNEEDSLEDDRLRLIFTCCHPSLAPEAHVALTLREVCGLTTEEIAKAFLITPRTLA
                     QRIVRAKAKIRETPIPYEVPTPQELPERLGAVLQVIYLVFNEGYSAAAGAEVTRAELT
                     GEAIRLGRLLAELRPEPEVMGLLSLMLLQESRRAARTSPTGELILLENQDRSLWNREQ
                     IAEGVALLEKALKSRRFGSYTLQAAIAAVHAEAESVAVTDWRQIVALYNQLVRIQPSP
                     VVQLNRAVAIAMCDGPEAGLRHIDAVLEHGELANRGIYLTPPTPRYSVLGHARGLSED
                     AT"
     gene            2665..2838
                     /locus_tag="VFC15_00665"
     CDS             2665..2838
                     /locus_tag="VFC15_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08706.1"
                     /translation="MAQTPEAPTNGSAHSPELYCPNCALEVDDPLTCGDCSAVICRVC
                     GTPLEMPEDLGIG"
     gene            2842..>4230
                     /locus_tag="VFC15_00670"
     CDS             2842..>4230
                     /locus_tag="VFC15_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM08707.1"
                     /translation="MAQPTTAQPAVAAHREPSLVRGLSLTDSVMLLAGGIIGSGIFLT
                     AGPIASAVRTPWFFLGVWLAGGLISLLACFAFAEMGAMFPEAGGQYVFLREAYGEFPA
                     FLYGWMIFTVGQSGTIAALAVGFAEYFGAVLPFASAHVPVATIFGWTLTRGHVVSICA
                     IVLLTAINVLGLKRGSALINVATWAKFAAIGLFIVLGLAIGHGHWSNYAVSIPASQPM
                     SLGQMISAFGVALIGVFWAFDGWVYVTWVAGEIKNPQRVLPRAMVMGVLLVGVIYLAV
                     NAVYLYALPITAVAKEETIAHAAAAALLGGSAAQWLSAVIALSCFGAMSCAILSSARV
                     SFAMARDGVFFHAMGRVHPRYRTPAFSLISLSAWAAVLALSGSFDQLYTYVMFMMVLS
                     YTAAVAGLFVLRRTHPDLPRPYRCTGYPYLPALYVLVAGAWALNSVVTRPKETLIGIA
                     IVFLGAPFYFFWR"
BASE COUNT          790 a         1352 c         1191 g          897 t
ORIGIN      
        1 tgaattcccg atattgtcca gaagccggct tctgggagat ctggccggat taggaacgag
       61 aaacgcccat gtcgccagaa tggattctcg ggcgtaaaat cccgcgtcag ggggaggttc
      121 tggagggtgc cgtgaacctg ctatttcttg tggctccggc cttttctttg agatctgatc
      181 gagtccaacg gggcacggga acatttacgg cgaaattagc aattaggggt atttacttga
      241 cgccaccaga cgcaacaggt tgtggtttga ccaaggcggc aaacgggatg gggcctacct
      301 gcacggcttg ttgagccaat cgatagaaca gtttgccgcg ggatttggag gtgcggcgat
      361 tgaatcgaaa agtgaactcg tccaggtaat cgtccagatg ctgcagtcca atcgcacctt
      421 ggtgggtgcc aagcagccat cttttcagca gcgcgatcac acgatggacg cgcggtagca
      481 gatgccctcc gtcttcttga tggctctgca cctggcggtc atgaacgtat ccttccaacc
      541 ccaaatagcc tctccagccg tcggaccgga ccgtgcttcc cggcgcaatc gcctctcgaa
      601 taaaaccgtg caggctcgcg ctggacgcat ccggtatcgt acgcagacga atgcgtccga
      661 tgccctttcc atcctcttcg gcggcgacga taatcagtgc cttttcctcc gcccccctgc
      721 cgattgcgcc ggtttcctct cctccccagt aggcctcgtc cacctcgaca acgccatcca
      781 agcggtcgcg gccaggtcga accatggcgc ggcgcaattt atgcagcatc gcccaggccg
      841 tcttgtagct gcccaagccg agaacacgct gtaaacccaa ggcgctcatg ccgttctttt
      901 ggctggtgat ctgccacatg gcccggaacc agatcgtcaa aggtacgtga ctatcctgaa
      961 agattgtccc tgcggtgacc gacatctcat accgacaatg gccgcaaagc caacgggtcc
     1021 tccgaatcgc ccaggcatct gctcccgcac accgtggaca ggcccatccg ccgggccaac
     1081 gcaatgccgc taggtactca acacaggctt cttcggtaca aaagcgtcgc tccagctcaa
     1141 gtagcgtctt cggataatcc tcgtgcatgg cccaaaacac tatatcttgg ggttggtggc
     1201 gtcaagtaaa tacccctatt tgccaattcg ccatgttcca agaccgcgtc gatatgccta
     1261 agaccggcct ctggaccgtc gcacattgca atggccacgg cacgatttag ctgtacaact
     1321 ggcgaaggct gaattcgtac caattggttg taaagcgcaa caatctgccg ccagtcggtc
     1381 acagcgaccg attccgcctc cgcatggacg gccgcaatcg cagcctgcag tgtgtaggag
     1441 ccgaagcggc gggacttcag ggctttctcc agcaatgcca ctccctccgc gatctgctcc
     1501 cggttccaga gcgagcgatc ctggttctcc agcaaaatca gctctccggt cggcgaggtt
     1561 cttgcggcgc gacgggattc ctgtagcaac atcagggaaa gcagccccat gacttccggc
     1621 tccggccgga gttcggcgag caaccggccg agtcgaatcg cctcgccggt gagctcggcc
     1681 cgcgttacct ccgctcccgc cgcagccgaa taaccctcgt taaagacgag atagatgacc
     1741 tgaagcaccg cgcccagtcg ctccggcaat tcctgcggcg tcggcacctc gtacggaatc
     1801 ggtgtctctc gaatctttgc cttggcgcgc acaatgcgct gcgccagcgt acgcggagtg
     1861 atgaggaagg cctttgctat ctcctcggtt gtcagcccgc acacctcacg caaggtgagc
     1921 gcaacgtgcg cttccggcgc tagagatgga tgacagcagg taaaaatcag gcgcagccga
     1981 tcatcctcaa ggctgtcctc ttcattggac ctttccgccg aactccattg cgcttcgaga
     2041 tatcgcacga gctcgtctcg agacgcttca aatcttgccc gtcgacgcag agtatcaatg
     2101 gctttgaatc gggccgtcga aatcaaccat ggtcgcgggt tgccgggtac tccactcctc
     2161 ggccacaggc tcagcgcagc cgcaaaggct tcgtgcatcg cctcctcggc aagatcaaaa
     2221 tcaccgagca agcggatcag agttgccagg attcgtcccg aatccacgcg ataaagggag
     2281 tcgagcaatt cgcgtatctg ctcggtggaa cgctcggaca tgacgacgac cttagcaaaa
     2341 ccagccaccc cgaatcaatt tggcaaacgg cggatcgatg caaagtgccc aggtcgtgac
     2401 tctgacatct ggacaacaga atcgcttcct cggttgacgc aaaatcgcca atgcactcat
     2461 tgagcttcgt acaagctcaa cccttctccg ttttgctccc gagtcacgcg caagccggaa
     2521 gttatcggtt cgtccgttgg attcgtaccg acaaacgccc ttcacgacac tttacttctg
     2581 cgtgtcgcca actcagggaa ctgacgcaca actatccgca aattgtttct ccgcaactca
     2641 tctgtgctac agtctccgaa accgatggct caaactcccg aagcacccac caacggctcg
     2701 gcgcattcgc cggaactgta ttgtcccaac tgcgcgctcg aggtggacga tccgctcacc
     2761 tgcggcgact gctcggccgt aatctgccgg gtctgcggca caccgctcga aatgcccgag
     2821 gacctcggga tcggctgatt catggcgcag cccaccacag cgcaacccgc cgtcgcggcc
     2881 catcgggaac cctcgctggt gcgcggcctc tcgcttaccg attccgtgat gctgctggct
     2941 ggcggcatca tcggctctgg catctttctc accgccggtc cgattgcctc cgccgtacgc
     3001 acgccgtggt tcttcctcgg agtgtggctc gcaggcgggc tgatctcgct gctggcgtgc
     3061 ttcgcctttg ccgagatggg agcgatgttc cccgaggcgg gcgggcaata tgtctttttg
     3121 cgcgaggcct acggcgagtt cccggcattt ctctacggat ggatgatctt caccgtcggc
     3181 cagagtggca ccatcgccgc gctcgccgtc ggcttcgccg aatatttcgg cgcggtgctc
     3241 cccttcgcgt cggcccatgt tccagtcgca acgatttttg gatggacgct gacgcgcgga
     3301 cacgtcgtgt ccatctgcgc tattgttctg ctcaccgcga tcaacgtgct cggactgaag
     3361 cgcggttcgg cgctgatcaa cgtagcgacc tgggcaaaat ttgcggccat cgggctcttc
     3421 atcgtgctcg gccttgcgat cggccacggt cattggtcga actatgcagt cagcatccca
     3481 gccagccaac cgatgtcgct gggtcagatg atctccgcat ttggcgtcgc gctgatcggc
     3541 gtcttctggg cgttcgacgg ctgggtctac gtgacctggg tggcgggcga aatcaagaac
     3601 ccgcaacgcg tgctgccgcg cgccatggtg atgggcgtgc tcctggtcgg cgtcatctac
     3661 ctggcggtca acgcggttta tctttatgcc ctgccgatca ccgccgtggc caaggaagag
     3721 accatcgcgc acgccgccgc ggccgcgctc ttaggaggga gtgcggcgca atggctcagc
     3781 gcggtgatcg cactctcgtg cttcggagcg atgtcgtgcg ccatccttag cagcgcgcga
     3841 gtttcattcg ccatggcgcg cgacggcgtc ttctttcacg ccatgggacg cgtgcatccg
     3901 cgctaccgca cgccggcctt cagcctgatt tcgctgagcg cgtgggcggc agtgctggcc
     3961 ctcagcggca gcttcgatca gctctacacc tacgtgatgt tcatgatggt gctgtcgtac
     4021 acggcggcgg tggcgggatt gttcgtgctg cggcgcaccc accccgacct tccgcgtccg
     4081 tatcgctgca ccggctatcc ttatttgccc gctctctacg tgctggtggc gggcgcctgg
     4141 gcgctcaact cggtggtcac ccgtcccaag gaaacgctga tcggaatcgc gattgttttt
     4201 ctcggcgcac cgttctattt cttctggcgc
//
LOCUS       DASRKK010000034         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1174561, whole genome shotgun sequence.
ACCESSION   DASRKK010000034 DASRKK010000000
VERSION     DASRKK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174561"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            828..2822
                     /locus_tag="VFC15_00675"
     CDS             828..2822
                     /locus_tag="VFC15_00675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM08708.1"
                     /translation="MKIHSKVFALLLFSASMLYAQGQAPAAATGGPQAPERLAADTPK
                     TTVMGNTFIAPKDWTLTVKGPATILEAPEGGSWIALVDVAAKDDDEALAKAWKAYRPE
                     AKWPIKVSNDLPDRDGWSKRRVYEYLTSPNEKREVAVLVFYSGTSWTVVIEDMADAVA
                     EKRGGQIGVALGRLLPKGYSRESFANKKANTLDQARIAELGRFIERGQKETGVPGVTL
                     GLVQDGKVIFAEGFGVKELGGTAKPNGDTLFMIASNTKALTTLMLAKLVEEHKMNWDT
                     QVTTLYPDFKLGDAATTQSVLVKHLICACTGMPRQDLEWLFEYGKMTPQSSMTLLGTM
                     QPTSKFGELFQYSNLMAAAAGYTGGHILYPNMDVGKAYDKAMQTLVFDPLVMSETTFD
                     FRKAEKANHATPHAPDIDGNPAKALMAVNYSVIPVRPAGAAWSSVDDMLKYVSMELAD
                     GKLPDGKTYIDKEPLLERRRPQVYMSKDDSYGMGLMVDKTYGVPVVHHGGDMIGYHSD
                     MIWLPQQNVGAVILTNGDPGWLIRTRFRRKLLEVLFDGHQEADNLLAADAKSYFAEIA
                     AERKLLTVPADATESEKLAKHYSNASLGDLDVSMSSGKTYFDFGEWKSEVATKKNPDG
                     TISFVTTVPGMSGFDFVVGTGAKKTLTTRDAQHEYIFEEK"
BASE COUNT          742 a          921 c         1058 g          650 t
ORIGIN      
        1 gaagtaatcc gtttcgtcga tcacgcggcc cgttggcggc gattcttccg gtgttctctc
       61 gccgcttcca atttccgatc ccggtcggcc tgaatctcct gctggcgtcc ggtcagcata
      121 tccttcggcg tgatatagcc gcgcggatga cccactggct gcgtggcgat tgagcccaat
      181 acacgatggg agaagcacga agcccgcagg tgtccgcggt gtcgacgctg gttcctggga
      241 attcaggcgc agatgctgcg cgagcgggag atggtagccc gcttgaacgg caagccgctg
      301 gaaccgtgcg gcgagagttg tcggcttccg gcggaacgtc ggcgccgcct aaaactcgcc
      361 ctggggcaat cagcggcgat atgagacgtg gccactttaa tcgcagtccg cgggtcaagt
      421 cgcaagggcc acggcaggat ctccctcgag ttctggaggc tggaagcctg gatcgcattc
      481 tcgctgcgct gaggcggaat tagctccgcc gtccctcaaa gcgtccgact ccagctgcgt
      541 aacggcgaaa aaggtttggt ttggcgcaat agcgcgttag tatccgcagc acattcgcaa
      601 tcggtccggt aactttttgg ctggcaacac ggttgccgga agattgatgt gccgaacgcg
      661 accccgcacg gagttcggcc tcaccactgg ctgtcttccg agtatgctgt cgcgcatcga
      721 ttcctgaaat gtaaggcatc tcccgtaagt gaggacacga atgccccggt caccgcgcct
      781 atcgagcact acctgagaag gcccacctgg agtaccatat ccgcgctatg aagatacaca
      841 gcaaagtgtt cgcccttctc cttttcagtg cttccatgct gtatgcgcaa ggtcaggcgc
      901 ccgccgcggc cacgggaggt ccgcaggccc cggagcggct tgcggccgac accccaaaga
      961 cgacggtgat gggaaacacg ttcatcgctc caaaggactg gacgctgacg gtgaaaggtc
     1021 cggcgacaat cctcgaagct ccggagggcg gttcgtggat tgcgctggtg gacgttgcgg
     1081 caaaggacga tgacgaagct ctagccaagg catggaaagc atacaggcca gaggcgaagt
     1141 ggccaataaa ggtcagcaac gatcttccgg accgcgacgg ctggagtaaa cgccgcgtgt
     1201 atgagtacct gacctcgccg aacgagaagc gcgaagtggc ggtgctggtc ttttactccg
     1261 gcacgagctg gacggttgta atcgaagaca tggccgacgc tgtggccgag aaacgcggcg
     1321 gacagatcgg cgtagcgctg gggaggctgt tgccgaaggg ctattcacgc gaatcattcg
     1381 ccaacaagaa ggcgaacacg ctggaccagg cgcggattgc ggagctgggc cgcttcatcg
     1441 agcggggtca aaaggaaacg ggagttcctg gagtcacgct cggactggta caggacggga
     1501 aggtgatttt cgccgaaggt tttggcgtca aggaactggg tggaacggcg aagccgaatg
     1561 gcgacacgct attcatgatc gcgtcgaaca cgaaggcgct gaccacgcta atgctggcaa
     1621 agctcgtcga agagcacaag atgaactggg atacccaggt cacgacgctg tatcccgatt
     1681 tcaagctggg cgacgcggcg acgacacaga gcgtgctcgt caagcatctg atctgcgcgt
     1741 gtaccggaat gcctcggcaa gacctggagt ggctcttcga gtacgggaag atgactccgc
     1801 aaagttctat gacgctgctg gggacgatgc agcctacctc gaaattcggc gagctgttcc
     1861 agtattcaaa cctgatggcc gcggccgcag gctacacggg tgggcacatc ctgtatccga
     1921 acatggacgt cggcaaagca tatgacaaag cgatgcagac ccttgtgttc gatccgctgg
     1981 tgatgagtga gactacgttt gactttagga aggcggagaa ggcaaatcat gccaccccac
     2041 atgcgccgga catcgatgga aaccccgcga aggcgctgat ggcagtgaat tactcggtca
     2101 tcccggtgcg tccggcgggc gcggcgtgga gcagcgttga cgacatgctg aagtacgttt
     2161 ccatggaact ggccgacggc aagctgccgg acggcaagac gtacatcgac aaggagcctc
     2221 tgctggaacg gcgcaggccg caggtgtaca tgagcaagga cgattcgtac ggcatgggat
     2281 tgatggtgga taagacttac ggagtcccgg tggttcacca cggtggagac atgatcggct
     2341 atcacagcga catgatttgg ctgccgcagc agaacgtcgg agcggtcatc ctgacaaatg
     2401 gcgacccggg gtggctgatc cggacccggt tccgccgcaa gctgctggag gttctcttcg
     2461 acgggcatca agaggccgac aatctgctgg cggcagatgc aaaaagctat ttcgctgaaa
     2521 tcgctgcgga gcggaagctt ctgacagtgc cggcggatgc cactgaaagc gagaagttgg
     2581 cgaagcacta ttcaaatgcg tcattaggag atctcgatgt gagcatgtcg agtgggaaga
     2641 cgtacttcga cttcggcgaa tggaagagtg aggtcgcgac caaaaagaac cccgatggga
     2701 cgatttcttt cgtcaccacc gtaccaggaa tgtcgggatt cgacttcgtt gtcggcacgg
     2761 gcgcgaagaa gacgctgacc acgcgagatg ctcagcacga gtacatcttc gaggagaagt
     2821 agccgggata gatccaacag ttcccacgtc gcgcgcttgc agccgaagaa caggacgcgc
     2881 tggttcttgt gaagccgcgc gggagcacaa ctcagcgaag cgtttggccg ggggcggtca
     2941 agttgacgag taacgcccga cggttccttc tggcgccctc cgccagagcg cgctgtcatt
     3001 ccgcgacatg ccgactattc gctcaagtgg atttccgccg cgaaatgcgc ccaatagcat
     3061 tcgttagacc ctggggcgtt tgtcagtact gatcgtgcat atcaattgct tttgatctca
     3121 agctggccgg tctctttgat ctagacaaac tggggtgtgg tgcataccct gagcacattt
     3181 gccgcttgac aataagttag tatttatata agttattgct tatgacagcc aggaatctgg
     3241 atgccgcatt cgctgctttg gccgatccaa cacggcgggc aattcttgcc cgcctcgcca
     3301 agggtgaagc gacggttaca gaactggctg agcccttcga catgacgcaa ccggcgatct
     3361 cccaacacct g
//
LOCUS       DASRKK010000035         6504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_197194, whole genome shotgun sequence.
ACCESSION   DASRKK010000035 DASRKK010000000
VERSION     DASRKK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197194"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..624
                     /locus_tag="VFC15_00680"
                     /pseudo
     CDS             58..624
                     /locus_tag="VFC15_00680"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(659..2122)
                     /locus_tag="VFC15_00685"
     CDS             complement(659..2122)
                     /locus_tag="VFC15_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZM08709.1"
                     /translation="MKWLTLATGVAASALLVGCVVGPKYRRPPVQAPTAYKTEGPWRV
                     AAPKDSLPKGAWWEIFNDAQLNNYEQQLLKANQSLAAAQDRLNQARALARVASAGLFP
                     QASTDPNASRNRYSGHRPEVVSLGRPLTQSTYEIPFLLSYEPDLFGKYRRTLEASNAT
                     LQATAADMYNVNLVLTAELAADYFSLRELDAETQVVQESVAIQQKGLQLVENRHTGGV
                     ASGLDLAQQQTLLDSTVTQLYLVQQQRAQYEHAIAVLTGNAASTFSVPVAPLHTTPPP
                     VPLGVPSDLLERRPDVATAERNMAFENAEVGVATAAFYPQLTIFGGGGVQSTSIANLI
                     SAPSAIWSLGGDLLQPIFQGGRNRANLAATKAAYDESVANYREAVLTAFQQVEDGLSG
                     LNALSQATTSQNAAVSDARRALQIANNRYVGGVTTFLDVITAQSALLTNERLATQLLG
                     QEMVTSVYLVKAVGGGWDASQIQNEQVRPKAIQAIQQ"
     gene            complement(2119..3345)
                     /locus_tag="VFC15_00690"
     CDS             complement(2119..3345)
                     /locus_tag="VFC15_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM08710.1"
                     /translation="MDSRSNQTDVVEPEQHAAEITPPPKLPAAAPRKALLMIAAVLLV
                     LVVGAVIAMLMRRHDSDVLAKETERESVPTVAVIHPTSEKPDEELVLPGSLQAFEESP
                     IYARTNGYLLRWYKDIGSRVTKGDLLADIDTPEVDQELMQARATRQQIVAQMDLAKIN
                     SDRYLSLRTTDSVSQQEADQQASGYRQAKANLDANDANVRRLEELEAFKHVYAPFSGV
                     LTKRNVDPGALINASGTGKELFDIARVDPLRVYVNVPQAYAPAIKNGMEAWVTLQELP
                     GQKFKGTVARTAESIDPITRTLLTEVDVPNKDGHLLPGSFGEVHFRPGISGQKVTLPV
                     NAMLFRQEGTRVAVVGSDNKVRLRPITIGRDYGNTLEILGGVSVEDQVVVNPADSLEE
                     GQQVNVASQSQGGNPS"
     gene            complement(3708..>6504)
                     /locus_tag="VFC15_00695"
     CDS             complement(3708..>6504)
                     /locus_tag="VFC15_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM08711.1"
                     /translation="GTTPPLVISYSASSTPVVQLGLSSKTLPEQQLFDLGQNFLRTQL
                     ATVHGAAMPYPYGGKIRQIQVDLDLPRLQANGLSPTDIVNAINTQNLITPSGTAKVGP
                     LEYQVEMNSSPQSIADLNDLPVKTVNGSTIYMRDVAHIRDGFAPQTNIVRQDGVRGTL
                     MSVYKIGSASTLSIVDAVKKTIPIAAQSLPRDITIKPLFDQSLFVRASINGVLKEAVT
                     AAALTAVMILMFLGDWRPTIVISISIPLSIFVSVILLSALGETINIMTLGGLALAVGI
                     LVDDATVEIENIERNLAQGKEMKRAILDGASQIAVPAFVSTLCICIVFVPMFFLTGVA
                     RYLFVPLAEAVVFAMLASYFLSRTLIPTLVMYIMRGHEHRAAGPKTIFGRYQRGFERG
                     FDKFRNAYYQLLETTLENRKLFAVCFLAFCVLSLGLVFFLGEDFFPTVDAGLMRLHMR
                     ARAGLRVEETARLADEVEKYLRQQIPKDEVVTILDNIGLPYSGINLSFSNSGVIGTSD
                     AEILVGLNAEHHHSTGDYIRKLRENLPRRFPGVEFFFQPADIVTQILNFGLPAPIDIQ
                     VTGQDMQGNYVIAQQIANRLRHVNGAADVHVQQMLSLPTLHLQIDRTKTTQVGMNARD
                     VAQSVLVSLSGSFQTAPNFWLNPKNGVTYQVAVQSPQYRMTSLQDLMDMPVNDPGVQN
                     SQVLGNLVQLTPVARPVVVSHYNVQPVIDVYESTQGRDLGAVAKDTMKVLQPFEQHLP
                     RGTSIIVRGQVETMQSSFIGLGIGLLMAISLVYFLIVVNFQSWLDPFIIITALPGALA
                     GICWILLLTHTTLSVPSLTGAVMCMGVATANSILLVSFARERMNAGIPSAQAALEAGY
                     TRIRPVLMTALAMIIGMVPMSLGLGEGGEQNAPLGRAVIGGLLFATVATLFFVPSIFA
                     MIHGHREAKREREAISS"
BASE COUNT         1300 a         1954 c         1939 g         1311 t
ORIGIN      
        1 gatgggactg cggcttaaaa aaagtcactc cacgcccagg aacaagaaac cccggaagcg
       61 cggcagcgcc ggcaagcgtg gcaggaaaac ataaggcaga ttgatccgca gcaactggtg
      121 tttctggatg agagcggggc ccgcacgcag atgacccgca attatggccg ggctccaggc
      181 ggagaacggg tgccggaagg cacaccccag agccactggc ataccgtgac catgctggcg
      241 gcgctcacca cccgcggctt gcagtcgccc atgaccatcg agtcgccgac cgatggcgat
      301 gtcttcctgg cctatctgga gcaggtcttg tgcccgcagc tccagcccgg acaggtggtc
      361 atcatggaca atctgtcagc gcacaaagtg gccggggtac agcaactcat cgccgcccgc
      421 ggagcgcggc tgatttactt gccgccctat tcccccgact tcaatccgat cgagcaagcc
      481 tggtcgaaga tcaagcagtt gctgcgctcc gccaaggccc gcaccctgga agctctggag
      541 agcgccatcg ccgaagccct ggcagcgatt actgccgaga acgccttagc ttggttctct
      601 cattgcgggt atggtctaca ataacgttga aaatgctcta gctaattgct agttgctcct
      661 actgctgaat cgcctggatc gccttcggcc gcacttgctc gttctggatt tgtgaggcat
      721 cccaaccacc gccaacggcc ttcaccagat agacggaagt caccatctcc tgcccgagta
      781 gctgcgttgc caggcgctcg ttggtcaata acgccgactg cgcggtgatg acatccagaa
      841 atgtagtcac gccgccgacg tagcggttgt tggcgatctg cagcgcacgt cgcgcgtcgg
      901 acacggccgc gttctggctc gttgtcgcct gcgaaagtgc gttcaacccc gacagcccat
      961 cttccacctg ctggaatgcg gtcagcactg cctcgcgata gttggcgacg gactcgtcat
     1021 aggcagcttt agtagcggca aggttagcgc gattacgtcc accttgaaag atgggttgca
     1081 gcaagtcgcc gcccagcgac cagattgcgc tgggagcgct gatcaggttg gcgatgctgg
     1141 tgctttgaac gccgccgcca ccaaagatgg tgagttgcgg ataaaacgcc gcagtggcaa
     1201 cgccgacttc ggcattttcg aacgccatgt tgcgctcagc cgtcgcgaca tcgggccgcc
     1261 gttccagtag gtcagaaggc acgcccagcg ggaccggagg aggtgtcgta tgcagtgggg
     1321 cgaccggcac gctgaaagtg gaagcggcgt ttcccgtcag caccgcaatg gcgtgttcgt
     1381 actgcgcgcg ctgctgctgc accaggtaca gttgcgtgac cgtggaatcc agcagtgtct
     1441 gctgctgtgc cagatcgagt cctgaggcga ctccgccggt atgacggttc tccaccagtt
     1501 gcaatccctt ctgttggatg gcaaccgatt cctgcactac ctgggtttcg gcatcaagct
     1561 cgcgcaagct gaaatagtcg gcggcgagtt ccgcggtcag caccaggttg acgttgtaca
     1621 tgtccgccgc cgtggcttgc agagtcgcat tcgaggcttc cagagttcgc ctgtattttc
     1681 caaagagatc gggttcgtaa ctaagcagaa aaggaatttc gtaggtgctt tgggtgagcg
     1741 gccgccccaa ggaaacgact tccggtcgat gtccagagta gcggttgcgc gatgcattgg
     1801 gatcggtgct ggcttgcggg aagagtccag ccgaagctac ccgcgccagc gcgcgcgcct
     1861 ggttcaggcg gtcttgcgct gcggccagcg actgattggc cttcagcaat tgctgctcgt
     1921 agttgttcaa ctgcgcatcg ttgaagatct cccaccatgc gcctttggga agcgagtctt
     1981 tcggcgcggc gacacgccac ggaccctccg tcttgtacgc cgtgggggcc tgaactggcg
     2041 gacgccgata cttcggtccc accacgcatc ccaccagcaa agcgctggcg gcgacgccgg
     2101 tggccagggt caaccacttc atgacggatt gcctccctgg ctttgggaag cgacgttgac
     2161 ctgttggcct tcttccagcg aatcggcggg attgacgacc acctggtcct cgacactgac
     2221 gccgcccagt atctcgagcg tattgccgta atcgcggccg atggtgatcg ggcgcagtcg
     2281 caccttgttg tcgctgccaa ccacggccac acgcgtgcct tcctggcgga agagcatggc
     2341 gttgaccggc agcgtgactt tctgcccgct gatgcccggc ctgaaatgaa cttcgccgaa
     2401 ggagcccggt aataggtgtc catctttgtt aggcacgtcg acctccgtca gcaacgtgcg
     2461 cgtgatggga tcgatggatt ccgcggtgcg cgccaccgtg cccttgaact tctgtcccgg
     2521 taactcctgc aaggtcaccc aagcttccat cccgttcttg atggccggag cgtaggcctg
     2581 cggaacgttg acgtaaacac gcagcgggtc cacgcgagca atgtcaaaca gctctttgcc
     2641 ggttccgctg gcgttgatca atgcgccggg atcgacgttg cgtttggtca ggactccgct
     2701 gaacggcgcg tacacgtgtt tgaacgcctc cagttcttcc agacggcgaa cgttggcgtc
     2761 atttgcgtcc aggttggcct tggcctgtcg atagccgctg gcctgctgat cggcttcctg
     2821 ctgcgaaacc gaatcggtgg tgcgcaggct gagataacgg tccgaattga ttttggccag
     2881 gtccatctgc gccacgatct gctggcgcgt cgcccgggcc tgcatcaact cctggtccac
     2941 ttccggagta tcgatgtcgg ccagcaggtc gcccttggtc acccggctgc caatgtcttt
     3001 gtaccagcgc agcaggtagc cgttggtgcg ggcgtagatg ggagactcct cgaacgcctg
     3061 caacgaaccc ggcaacacca gttcctcgtc aggcttctcc gacgtgggat gaatcacggc
     3121 cacggtcgga accgattcgc gctccgtttc cttcgccaga acatcgctgt cgtgcctgcg
     3181 catcagcatg gcgatcaccg cccctaccac caacaccagc agcactgcgg cgatcatcag
     3241 cagtgccttg cgcggcgctg ccgcaggaag cttgggcggc ggcgtgattt cggccgcgtg
     3301 ctgttcgggt tccacaacgt cagtttgatt tgaacgagaa tccatatgtg agtccttcgg
     3361 actggcaatt agcagttagc aattagtcaa accgtgcgac tctgtcgcta gttgctagtc
     3421 cgttcgtgcc gcctgttttc aatccttcga attgccgggc ctgtaagttc ctaatccatt
     3481 agttgccaac tgccagctca acgccgctaa atactacaag ccatctcata aataccggag
     3541 cgggccaaac cgaagtacct caggggctaa agcccagact gtaaatgacc atttgcggca
     3601 cggctgaagc cgtgcccttt caaaaccgat ttatcagatg ccttctagct gccaattgct
     3661 aactgctaat tgctaactgt aaattgctaa ttgctaactg ctaattgcta actgctaatt
     3721 gcttctctct cccgcttcgc ctctcgatgg ccgtgaatca tggcaaagat ggatggcaca
     3781 aaaaacaggg tcgccacggt ggcgaacaac aatcccccga tcaccgcgcg tcccaacggc
     3841 gcgttctgct ccccgccttc gcccaggccc agcgacatcg gcaccatgcc gatgatcatc
     3901 gccagggcag tcatcagcac cggacggatt cgggtgtagc ccgcttccag cgcggcttgc
     3961 gccgaaggta taccggcatt catgcgctcg cgcgcaaacg aaaccagcaa gatcgagttg
     4021 gccgtggcca cgcccatgca catcaccgcg ccggtcagcg acggcacgct caacgtggta
     4081 tgcgtcagca gcaggatcca gcagatgccg gccagcgctc ccggcagggc ggtgatgatg
     4141 atgaacggat cgagccatga ctgaaagttc accacgatca ggaagtacac cagcgagatc
     4201 gccatcagca gtccgatgcc caagccaatg aacgacgact gcatggtctc cacctgcccg
     4261 cgaactatga tgctcgtgcc gcgtggcaaa tgctgctcga aaggctgcag caccttcatc
     4321 gtgtccttgg ccaccgcgcc caggtcacgg ccctgcgtgc tttcgtaaac gtcaattacc
     4381 ggctgcacgt tgtaatgcga gaccactacc ggcctggcga ctggagtcaa ctgcaccagg
     4441 ttgcccagca cctgcgagtt ctgcacaccg ggatcgttca ccggcatgtc catcaggtct
     4501 tgcagcgagg tcatgcggta ttgcggagac tgcacggcca cctggtaggt cacgccgttc
     4561 ttcggattca accagaagtt aggcgcggtc tggaaactgc cgctcaacga gaccagcacg
     4621 ctctgcgcca cgtcgcgcgc attcattccc acttgcgtgg tcttggtgcg gtcaatttga
     4681 agatgcaggg tcggcaaact gagcatctgt tgcacgtgca catcggcagc tccgttcacg
     4741 tgacgcaggc ggttggcgat ttgctgcgcg atgacgtagt tgccttgcat gtcctggccg
     4801 gtgacctgga tgtcaatcgg cgcgggcaat ccgaagttca gaatctgggt cacgatatcg
     4861 gccggctgaa agaagaactc gacgccgggg aagcggcgcg gcagattttc ccgcaatttc
     4921 cggatgtagt ccccggtgga gtgatgatgc tcggcattga ggccgaccag gatctccgca
     4981 tcgctggtgc cgatgacgcc cgagttgctg aatgacagat tgatccccga atacggcagc
     5041 ccgatattgt cgaggatggt caccacttcg tctttcggga tctgctgccg tagatacttt
     5101 tccacctcat cggccagccg cgccgtctct tccacgcgca ggccggcgcg ggcgcgcatg
     5161 tgcagacgca tcaggccggc gtccaccgta gggaaaaagt cttcgcccag gaagaaaacc
     5221 agtcccagcg aaagcacgca gaatgcgaga aagcagacgg cgaacagctt gcgattctcg
     5281 agggtagttt ccaggagctg gtagtacgcg ttgcggaact tgtcgaaccc gcgctcgaag
     5341 ccgcgctgat agcggccaaa gattgttttg ggcccggcgg cacgatgctc gtgcccgcgc
     5401 atgatgtaca tcaccagcgt agggatcaaa gtccgcgaaa ggaaatagga tgccagcatc
     5461 gcgaacacga cggcttccgc caacggtacg aacaggtagc gggcaacgcc ggtgaggaag
     5521 aacatgggca cgaacacgat gcagatacac agggtggaca cgaacgccgg taccgcgatc
     5581 tgggaagcgc catccaggat ggcccgcttc atctccttgc cttgcgcaag gttgcgctcg
     5641 atgttctcga tctcgaccgt ggcgtcgtcc accagaatgc cgaccgccag ggccagacct
     5701 ccgagcgtca tgatgttgat ggtctcgccc agcgcactca gcagaattac cgaaacaaaa
     5761 atggagagcg ggatggaaat ggagatgacg attgtgggac gccagtctcc cagaaacatc
     5821 aggatcatga ccgcagtgag agcggcggcg gtcaccgcct ccttcaacac accgttgatg
     5881 gaagcgcgca caaacagcga ctgatcgaac agcggcttga tggtgatgtc gcggggcagg
     5941 gactgcgcgg caatcggtat ggttttcttt acggcatcca caatggagag ggtggaggcg
     6001 ctgccgattt tgtagaccga catcagcgtg ccacgcacgc catcctgccg cacgatgttg
     6061 gtttgtggcg caaagccatc gcggatgtgg gccacatcgc gcatgtatat cgtcgaaccg
     6121 ttcaccgtct tgactggcag atcgttcaaa tccgcaatgc tctgcgggct gctgttcatc
     6181 tcgacctggt attccagggg ccctaccttg gcggtgccgc tcggagtaat caggttttgc
     6241 gtgttaatag cgttgacgat gtcagtggga gaaagtccgt tggcctgcaa ccgcggcaga
     6301 tcgaggtcaa cctggatctg gcgtatcttg ccgccgtacg gatagggcat ggccgcgcca
     6361 tgtacggttg ccagttgggt gcgcagaaag ttctggccca ggtcgaacag ttgctgttcc
     6421 ggcagggtct tgctgctgag tccaagctgc accacaggcg tggacgacgc cgagtaggag
     6481 atgaccagcg gcggcgtggt gccc
//
LOCUS       DASRKK010000036         5004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_99561, whole genome shotgun sequence.
ACCESSION   DASRKK010000036 DASRKK010000000
VERSION     DASRKK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99561"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..3009)
                     /locus_tag="VFC15_00700"
     CDS             complement(4..3009)
                     /locus_tag="VFC15_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZM08712.1"
                     /translation="MSTNPAVEAVIEQEKLFEAFENLMPFRVQDILLVSSLYDSFILR
                     EDGRLNEMLIGESYELNLQHVPRITHVKTGAEALALARADSRFNLIVSNLEVEDMTAA
                     DLATEVKRAGLDVPVVVLAYDYREVKNFVARTPNSPIERIFLWQGNARILISIIKYIE
                     DNRNVEHDTEVMGVPVMLVVEDNIRYYSSFLPVIYTELISQARRLISEGLNVAHKLVR
                     MRARPKILLASNYEDAAHLVLLYRENLLGVVLDVEFPRKGKLMPETGFELARMIRSIV
                     PDVPIVLQSGRPEFMAKAHAEEFSFLQKRSPTLLGDLRHWLIDQVGFGPFIFRLPDRK
                     TEVARATNLNELERALRTVPAESISYHAGRNHFSHWLMARTEFAVAQKLRPRKVSDFA
                     SIEVLRNDLIEAITSYRRDQSQTLVGDFNAETFQPTDPFFLRIGDGSLGGKARGLAFA
                     RHLLQPKHMPRRFSGVRIAVPRTVVLATDVFDRFISENDLHDFAMRSSSDDEVVKRFL
                     ATPLPEAIETDLRAFLENVTFPLAVRSSSLLEDSQYQPFSGVYDTFMLGNHDRDIEVR
                     LRQLTEAIKRVYASTFSQHSKAYVRATPYRLEEEKMAVLIQQVVGTAHGQRYYPDFSG
                     VVRSRNFYPVAPLTVKDGFAAVALGMGRAVVGGGKCLTFSPRYPRHLLQFSSVEDILA
                     NSQTEFWALELVPRVHHEDPADDLREVSFPLKTAETDGTLHMLASTYSVENHAVYDGL
                     SRPGVRIVSFAPILKHGLFPLPKILEQLMSIGEDAMGRPVEIEFAVKLAARAGQDAEF
                     GFLQMRPLVMSREGEELRMEDVEPSQLICQSAMVLGHGRIQDLHDIIVVDFNRFERAK
                     SHDVAQSVAHFNSQLSAQGKPYALIGVGRWGSNEAWLGIPVTWDQISGARVIVEAGFR
                     DFRVTPSQGSHFFQNLTAFQIGYFTVNPDAGEGFVDWEWLNAQNGIEANGCVHLLHFD
                     KPVSVVMDGKTSRGMIFKPEVEDPTSATG"
     gene            3094..3855
                     /locus_tag="VFC15_00705"
     CDS             3094..3855
                     /locus_tag="VFC15_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08713.1"
                     /translation="MSFLDDFGFKPMRLGIAVIVIIMAATAYNTWRNARMDALAHAAV
                     DKKTDATAVKRLAGYSGQRASELLLVVASGAQTEATRLAALQALVDRKDAARVSRLSE
                     MLLPPESFAVRQALANAIYQTGCSPECVRNILYFEERMWRGDRMSEETAPNSPTDLSE
                     KELELQTALDEILRKNKQALGVMLERSYGLAGSFPSPFATETVTRLGITEACPLLMRT
                     YLTVHENVKASPEYKNVTDAVDKLGCKNQPIRSQP"
     gene            complement(3928..4491)
                     /locus_tag="VFC15_00710"
     CDS             complement(3928..4491)
                     /locus_tag="VFC15_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM08714.1"
                     /translation="MPPGKVAAVVTYLEMRTPPFPSTPAMSEFAVRHVSQPDLDWYRE
                     LYRVIGEPWLWFSRLRMSDEELRAVIHDPAVDVFALTHASEDHGLLEFDRRRFPDIEL
                     SFFGVTPALIGKGAGSALLQHCLPLEWEYKPQRVWLHTCTSDHPGALAFYVKAGFVPY
                     KRAIEISNDPRLTGEMPRTAAHHIPIV"
     gene            4773..5004
                     /locus_tag="VFC15_00715"
                     /pseudo
     CDS             4773..5004
                     /locus_tag="VFC15_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1024 a         1457 c         1526 g          997 t
ORIGIN      
        1 gatctatccg gttgccgaag ttgggtcttc gacttccggc ttgaagatca tcccgcggct
       61 ggtctttcca tccatgacta cggaaacagg cttatcgaaa tgcaggaggt gcacgcaccc
      121 gtttgcctcg atgccgttct gcgcgttcag ccactcccaa tccacgaagc cttcaccggc
      181 atcgggattc accgtgaagt agccgatctg gaatgcggtc aggttttgaa agaagtggct
      241 gccctgcgat ggtgtcactc taaaatcgcg gaagccggct tcgacaatca cccgcgcgcc
      301 tgaaatctgg tcccaggtga caggaatgcc gagccacgct tcgttcgatc cccagcgtcc
      361 gacgccgatc aaggcgtacg gcttgccctg cgcgctcaac tgggagttga agtgggcaac
      421 gctttgggcc acgtcatggc ttttggcgcg ctcgaagcgg ttgaagtcca ccacgatgat
      481 gtcgtgcaaa tcctggatgc ggccgtgtcc cagcaccatc gcactctggc agataagctg
      541 gctgggctcc acatcttcca tgcgtaactc ttctccctcc cgcgacatca ccagaggacg
      601 catctgcagg aagccaaatt ccgcgtcctg gcctgcgcgt gccgccagct tgacggcaaa
      661 ctcaatttcc actggacgtc ccatggcatc ttcaccgatc gacatgagtt gctcgaggat
      721 tttcggcagc ggaaacagtc catgtttcag gatgggcgca aagctgacga tgcgcacgcc
      781 cggacggctc agcccgtcat acaccgcgtg gttctccacc gagtaggtgg acgccagcat
      841 atgcagcgtt ccgtctgtct ccgccgtctt gagaggaaat gaaacttcgc gcaaatcgtc
      901 agccggatct tcgtgatgaa cacgcggcac cagttccagc gcccagaatt ctgtttgcga
      961 gttggcgagg atatcctcga ctgacgaaaa ctgcagcagg tgtcggggat aacggggact
     1021 gaacgtcagg cactttccgc cgcccaccac ggcgcggccc atgccgagag cgacggccgc
     1081 aaatccgtcc ttcaccgtca gtggtgctac cggataaaaa ttgcgcgagc gcaccacacc
     1141 cgagaagtcc ggataatacc gttgaccgtg cgccgttccc accacctgct ggatcagcac
     1201 cgccattttc tcttcttcca gacggtacgg cgtcgcccgc acgtaggcct tggaatgctg
     1261 gctgaaggtg gaggcgtaca cccgcttgat ggcttccgtg agctgacgca gccgcacctc
     1321 gatgtcacgg tcatgattgc cgagcatgaa ggtgtcgtac acgccagaga atggctggta
     1381 ctgggaatct tcgagcaggc tggacgaacg cacggccagc gggaatgtta cgttctccag
     1441 aaacgcgcgc aagtcggtct ctatagcttc gggcagcggg gttgcgagaa agcgcttcac
     1501 gacctcatcg tcactggaag agcgcatagc aaagtcgtgc agatcgtttt cactgatgaa
     1561 gcgatcaaac acgtcggtgg cgagcactac cgtgcgtggg acggcaattc gcactccgga
     1621 aaaacgccgc ggcatgtgct tgggctgcag caggtgacgc gcgaacgcca gcccacgtgc
     1681 cttgcctccc agcgaaccat ccccgatgcg caggaagaac gggtcggtgg gctgaaacgt
     1741 ttcggcattg aaatcaccga caagcgtctg gctctggtcc cgccgatagc tggtgatcgc
     1801 ctcgatcagg tcgttccgca gcacctcaat gctggcgaaa tccgacacct tgcgcggccg
     1861 cagcttctgc gcgacggcaa actcggtccg cgccatcagc cagtgcgaaa agtgattgcg
     1921 cccagcatgg tatgaaatgc tttccgccgg gaccgtgcgc agcgcccgct ccagttcatt
     1981 caggttggtg gcgcgcgcca cttcggtttt gcggtcgggc aggcggaaga tgaagggccc
     2041 gaatccaact tgatcgatca gccagtgacg caagtctccc agcagggtag gcgaacgctt
     2101 ttgcaggaag gaaaattctt ccgcgtgcgc cttcgccata aactccggac gcccggattg
     2161 caacacgatg ggcacgtcgg gcacgatgct gcgaatcatg cgcgccagct cgaaaccggt
     2221 ttcaggcatg agtttgccct tgcggggaaa ctccacatcc aataccacgc cgaggaggtt
     2281 ctcgcgatac agcagaacga gatgggcggc atcttcgtaa ttcgacgcca gcaggatttt
     2341 gggacgcgcg cgcatgcgca ccagcttgtg agctacgttg aggccttcgc tgatcaaccg
     2401 ccgcgcctgg ctgataagct cggtataaat caccggcaga aacgatgagt agtagcggat
     2461 gttgtcttcg actaccagca tcacgggcac gcccatgacc tcggtgtcgt gctcgacgtt
     2521 gcgattgtct tcgatgtact tgatgatcga gatcaggatg cgcgcgttgc cctgccacag
     2581 aaagatgcgc tcgatgggac tgttgggcgt gcgcgctacg aaattcttga cctcgcgata
     2641 gtcgtaagcc agaacgacta cgggcacatc caaaccggcg cgcttgacct cggtcgccag
     2701 gtcggcggcc gtcatgtcct cgacttcgag gttggagacg atcaggttga agcgcgaatc
     2761 ggcccgcgcc agcgccaggg cctcggcacc ggtcttcacg tgcgtgatgc gcggaacgtg
     2821 ctgcaggttg agctcgtagg attcgccgat cagcatctcg ttcaggcgac cgtcttcgcg
     2881 cagaatgaac gagtcgtaca agctcgacac caaaaggatg tcctgcaccc ggaagggcat
     2941 caggttttcg aaggcttcga agagcttttc ctgttcgatg acggcttcga ccgctggatt
     3001 ggtgctcatg gtgcaatagt aactcgcatg cattgggatg tgcggtgata ccgttcaccc
     3061 aggagcggtc atttgatacc gtagaagcac gctatgtcat ttctggacga ttttggcttc
     3121 aaaccaatgc ggctcggcat cgcagtcatt gtgatcatca tggcggcgac agcctataac
     3181 acgtggcgca atgcccgcat ggatgcgctg gcgcacgcgg cagtcgacaa gaagacggat
     3241 gccaccgcgg tgaagcggct ggcggggtat tccgggcagc gtgcaagtga gttgttgctg
     3301 gtggtggcct caggtgcgca gacggaggca acccgcctgg cggcgctgca ggcgctggtc
     3361 gatcgcaagg acgcagcccg ggtgtcgcgg ttgtcggaga tgttgcttcc gcccgagagt
     3421 ttcgcggtac ggcaagcgct ggccaacgcc atctatcaaa ccggatgctc gccggagtgc
     3481 gtgcgcaaca ttctctattt cgaggaacgc atgtggcgcg gggacaggat gtcggaggaa
     3541 acggctccca attcgccgac ggacttgtcg gagaaggaac tggaattaca gaccgcgctt
     3601 gatgaaatcc tgcgcaagaa caagcaggcg ctgggcgtga tgctggaacg aagctacggg
     3661 ctcgccggct cgtttcccag tccgttcgct acggagaccg tcacgcgatt gggcatcaca
     3721 gaggcctgtc cgctgttgat gcgcacttat ctgacggtgc atgaaaacgt gaaggcttcg
     3781 cccgagtaca agaacgtcac cgacgcggtg gataagctgg gctgcaagaa ccagccgatt
     3841 cgcagtcagc cttagatcag tgcgattggc cgggtgagta aagtttcaag cttccagttt
     3901 cgagtttcaa gagtggcggc gccgagttca cacgatgggg atgtggtgag cggcggtgcg
     3961 aggcatttct cccgtgaggc gcggatcgtt gctgatttcg atggctcgct tgtacggcac
     4021 gaatccggcc ttcacgtaaa acgccagcgc gccaggatgg tcggaggtgc aggtgtgcag
     4081 ccagacgcgc tgcggcttgt actcccactc cagcgggagg cagtgctgca gaagagcgct
     4141 tcctgcgccc ttgccgatga gcgcgggcgt cacgccaaaa aacgacaact caatatcggg
     4201 gaagcggcgg cggtcgaact ccagcaggcc gtgatcttcg ctcgcgtgtg ttaacgcgaa
     4261 aacatcgacg gcggggtcgt ggatgacggc gcggagttct tcgtcggaca tgcgcagccg
     4321 gctgaaccac aaccacggct cgccaatcac gcgatagagc tcgcggtacc agtcgagatc
     4381 gggctgcgaa acatggcgca ccgcgaactc tgacattgcg ggagtcgatg ggaacggcgg
     4441 cgtgcgcatc tcgaggtaag tcactacggc ggcgactttt ccagggggca agtcggtgta
     4501 tccatcacgc atcagtgtaa agagtagcag acgcagtcgg agtcctgcgg gccctcgaac
     4561 cgatatagaa tgacccatca attcccggca gacgaggggt aggaatgatc tcgcggcggc
     4621 actttctgat catctcgggt ggtgtcgtgt tgatgccgca atactcctgg gcgtccgata
     4681 aaatccccct catgctcgat catcttttgc tgggctgcag cgatctaggg cgtgttaaca
     4741 ctaatcgata attttgtgtt atgctgcgcc ctatggagat caccgaggag cagtgccagc
     4801 gtattgcccc cagtcttcct cgtcagcgcg gcaatgtgcg ggtcacccat ctgcaatttc
     4861 tcaacgccct gctctatgtc accgagcacg gctgcaagtg gcgcggcctg cccaagcgtt
     4921 tcggcccgtg gcaccccatc tacacgcgca tgaacgctgg tccaagagcg gcgtactgga
     4981 caccgtcttc gcccagttgc agcg
//
LOCUS       DASRKK010000037        15129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_588238, whole genome shotgun sequence.
ACCESSION   DASRKK010000037 DASRKK010000000
VERSION     DASRKK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588238"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2806)
                     /locus_tag="VFC15_00720"
     CDS             complement(59..2806)
                     /locus_tag="VFC15_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZM08715.1"
                     /translation="MLVFELTKQWPHLSMTIRASQLIALGFTIVGIALSSVVAERGAN
                     SRRAMDAEVEQRRGSGGESRCETSAQGQVEIGFPQDEDLMRAAIEAARIGFLRWDVTH
                     DKQEWSATAKRLLGLSPESPADFTVLMDTVHREDRESLRRLITSVTPQKPEFSHEHRA
                     CWRDGTVHWIWIKGWGFFDEGGSLLTISGIAMDIDERKQAEDRLRLQATALEQAANAI
                     VLTDEKGAILWVNPAFTQMTGYRADEVIGRNPKLLQSGKQDAAFYGALWNTIAAGSHW
                     RGEIINRKKDGTLYTEDMTIAPVRSTSGPISHYVAIKQDVSQRRLAEQALQNAQQKYR
                     SIFDNAILGIFQTTPDGEFLAVNPALARMAGYESPEEFLNSVHCTAELYVDFNRRDEL
                     RELIKSQKVVRAFEVDFRCKDGSTRTTSLDIGAMADGKDGSFYLEGTVQDITQRKAAE
                     ARIQFLAYHDALTGLPNRALFEDRLAKALGNARRRGESVAVLWLDLDNFKTINDSLGH
                     SVGDSLLKQIGERLHKHARAQDTVAKVGGDEFVFALINPGNISRVTATAERIRRQVAG
                     EFEVQGHVLNVTCSIGISLFPDHGADCEALVKNADVAMYSAKKYGRNNYRFFTAELND
                     MAAERLVLESGLRVAIENREFFLQYQPQVDMATGRITGAEALLRWRHPVLGLVGPDKF
                     IPIAEESGLIVAIGEWVLTSACAQARNWQDQGLPTMHIAVNVSALQLHHQRFLHVVRR
                     VLRETGLAPKYLELEITESVLFANAGQVLSKLHQLTELGLRLSIDDFGTGFSSLSYLK
                     DLPVFRLKIDHSFIKTLTVDPRDAAITASIISMGHSLDLNVLAEGVETEAQMSFLRAH
                     HCDGIQGYFFSKPLEADDFAKKVRGTSLSQLEALASVPEPCTGALESQSPSRQITEAI
                     A"
     gene            3520..4473
                     /locus_tag="VFC15_00725"
     CDS             3520..4473
                     /locus_tag="VFC15_00725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM08716.1"
                     /translation="MELGSRIDGSNGFSAPIDDDSSSFHAKLLDSLFDGVYFVDAERK
                     ITYWNQGAESLTGYSSREAVGRHCYDNFLVHIDETGGALCTIGCPLTSTIHDGQRREA
                     DVFLRHKLGHRVPVCVRVAPITDPARQIVGAVEVFSDISAKKQVERRVHELEGMAFRD
                     GLTGLPNRSYIELKAKQSFEAFQQFQRNFGVMMFDVDHFKQVNDRHGHDAGDLVLKAI
                     SDTLAKGLRENDIVGRWGGEEFLALLVDVDACVLRQLAERCRSLVAGSGALSGDSRIT
                     ATVSVGATLFTQADSIQSAIARSDQLMYVSKSSGRNKITIG"
     gene            complement(5190..5552)
                     /locus_tag="VFC15_00730"
     CDS             complement(5190..5552)
                     /locus_tag="VFC15_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM08717.1"
                     /translation="MLAEDEKDVRSLIGGHLASLGYTVVEAGNGADALQLVADAPGFF
                     DLLVTDIIMPKMGGQELAGKIREICPTIKSVQISGYNETTQEPSDEVVPQGRRLQKPF
                     TLDTLDATVRQVLDHDQS"
     gene            complement(5632..5814)
                     /locus_tag="VFC15_00735"
     CDS             complement(5632..5814)
                     /locus_tag="VFC15_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08718.1"
                     /translation="MRTDLLQSVQQLFGTRCFAYHPKPVLDLQHLLYPGAKHVLMVSN
                     DDLNHSDASSLAELLA"
     gene            6274..6852
                     /locus_tag="VFC15_00740"
     CDS             6274..6852
                     /locus_tag="VFC15_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM08719.1"
                     /translation="MHLFLKLGIALAILAFATSSFAAPTTFDWYFASTAGHISGNGAL
                     TAVPDPSATAGLYDITGGAGTVTSHSVAFAVTIAPCLNYAATCTFVNTDGHGANLQVD
                     NLLYANHAPGSYLDGYGIVLTPAPLGSGASYIGIWDSPSQYFYNYTADGYNNLSTPFT
                     IESSATPEPSTLLMMGSGILGLAGLARKRLFS"
     gene            8701..8997
                     /locus_tag="VFC15_00745"
                     /pseudo
     CDS             8701..8997
                     /locus_tag="VFC15_00745"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
     gene            complement(9354..9554)
                     /locus_tag="VFC15_00750"
     CDS             complement(9354..9554)
                     /locus_tag="VFC15_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HZM08720.1"
                     /translation="MYHNPESMTSGFQCAGCGGWNETVVDASVGTRQSYVEDCQVCCK
                     PNVLRIDWDSESGEYVVSAELE"
     gene            complement(11038..11340)
                     /locus_tag="VFC15_00755"
     CDS             complement(11038..11340)
                     /locus_tag="VFC15_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08721.1"
                     /translation="MFNNDTVSGGVLAWLAATFIALVVVVVLCVLASTVYPRLSTAGL
                     IALFQIGLWQWLYLIPLIVWLRRRNRSELAKGVTIAACIAFGLNALCSGLLFFSSR"
     gene            complement(11330..12751)
                     /locus_tag="VFC15_00760"
     CDS             complement(11330..12751)
                     /locus_tag="VFC15_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM08722.1"
                     /translation="MNDRVRPYLFYDVAISICSRCFRKVEGKIVFCDGKVLMLKRCPV
                     HGIETVMMADDVDYYRKCREVFLKPPEQAAVYNTPVRWGCPYDCGLCPDHEQHSCLAL
                     VEITDHCNLRCPTCYSVSGPERPYYRTLEHVEQMLDAVVRNEQQPDIVQISGGEPTMH
                     PDFFAILDAAKRRPIRHLMVNTNGLRLAAEEEFAERLAGYMPDFEIYLQFDSLNAGPL
                     NALRGADLRNVRRRALERLNRLGISTTLVVTLQRGLNDDEIGRIIDFALQQPCVRGVT
                     FQPIQVAGRLEKFNPAADRLTLTEVRRRIYEQTSVFRPEDIVPVPCHPDSLAMAYALK
                     LDGKITPLTSLIPPEVLIEGGHNTVLYEQEQSLHDHIFRLFSTSHSPKSGAGTLHDLL
                     CCLPRVSAPSLSYDNVFRVIIMQFIDAYSFDVRSIKKTCVHIVHPDGRLIPFDNYNLF
                     YRDELEHTRLAALRAGVGAEHVQ"
     gene            complement(12748..13488)
                     /locus_tag="VFC15_00765"
     CDS             complement(12748..13488)
                     /locus_tag="VFC15_00765"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZM08723.1"
                     /translation="MIFIPSMARGPLHVIFELLGYLAGSLLYIRARQRSGDFLPDSLR
                     STIVCAAFVGAALGSRLLACLEHPQIFRWHALFLLDGGRTIVGGVLGGWIAVEAVKHK
                     YRIQSRTGDLFVTPLIVGLILGRIGCFFAGTADDTFGRPTTLPWGIDFGDGLARHPLQ
                     LYEILFLLAYILLSRELAWPPVNGDRFRLFVFSYFSLRFGLEFMQDSPRLLGLDALQW
                     ACLAGALWCAPTVLDIIRRVPHRLPETS"
     gene            complement(13896..14372)
                     /locus_tag="VFC15_00770"
     CDS             complement(13896..14372)
                     /locus_tag="VFC15_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZM08724.1"
                     /translation="MTKTQHPLVAVLGCSDSRVPPELVFDAGLGELFVVRSAGEDDGP
                     IELGSLEYAVEHLGISMIVVMGHQSCGAVTAACSRGKSESVNLDAVLKPIVPSCMKVD
                     PKQPETLDIAIRDHVHQVAQEALSRSEVLSKASEEHKLTIIEAYYSLESGEVTRIR"
BASE COUNT         3331 a         4277 c         4149 g         3372 t
ORIGIN      
        1 agtatcagat cgaagcaatc cgttcaccgc accctccctt acttgcccgc cctagttcct
       61 atgcgattgc ttctgtaatc tgacgacttg gggattgact ctctagcgcg cccgtacaag
      121 gttcgggcac agaggcgaga gcttccagct gcgacagtga cgtaccgcgt accttcttcg
      181 caaaatcatc cgcctccaaa ggcttgctaa agaagtatcc ttgaattccg tcgcagtggt
      241 gcgcccgcag gaaggacatt tgggcctcgg tctcgacacc ctccgcgagg acattcaggt
      301 cgaggctgtg tcccatgctg attatggagg ctgtaattgc cgcatcacgt gggtcgaccg
      361 tcaacgtttt tataaacgaa tgatcgatct tcaacctgaa gacgggaaga tccttcaaat
      421 aactgaggct ggagaatcca gttccgaagt cgtcgatgga caatcgcaac cccagctccg
      481 tcagttggtg taacttcgaa agcacctgcc cggcattcgc gaacagcacg ctttccgtga
      541 tttccaactc aagatacttt ggggcaagac cagtttctcg cagtacgcga cgtacaacat
      601 ggaggaagcg ctgatggtga agttgcagcg ctgatacgtt gactgctatg tgcatagtgg
      661 gaaggccctg gtcctgccag ttccgggcct gagcacaggc gctcgtgagc acccactcac
      721 cgatggcgac gatcaggccg ctctcctccg caatcggtat gaacttatct ggtccgacga
      781 gacccaacac cggatgtcgc cagcggagaa gcgcttccgc tcctgtgatc cttcctgtag
      841 ccatatctac ttgcggttga tactggagaa agaactcccg gttctctatt gcgactcgta
      901 agccactctc caaaaccagg cgctctgcgg ccatgtcgtt cagctccgcg gtgaagaatc
      961 gataattgtt cctaccgtat ttcttcgcgc tgtacatagc tacatccgca ttcttaacta
     1021 gggcctcgca gtccgcgcca tggtccggga acaagctgat accgatactg caggtaacat
     1081 tgagcacatg accttgaacc tcgaattcgc cggccacctg acgcctgatc ctctctgcag
     1141 ttgccgtcac gcgggagata ttgcccgggt taatcaacgc gaatacaaac tcgtctcctc
     1201 cgaccttagc caccgtgtcc tgcgcgcggg catgtttgtg caaacgttct ccgatttgct
     1261 tcagaagaga gtcgccaacc gaatgtccca gcgagtcgtt gatggtcttg aagttatcaa
     1321 ggtcgagcca caatacggcg accgactctc cccgccgtcg ggcattgcct agcgccttgg
     1381 ccagccggtc ctcaaaaaga gctcgattgg gcaggccggt tagcgcatcg tggtacgcca
     1441 ggaactggat tcgcgcctcg gcagctttgc gttgagtgat gtcctgcacg gtgccttcga
     1501 gatagaagct cccgtccttg ccatctgcca ttgcaccgat atctagcgag gtggttcttg
     1561 tgctgccatc cttgcacctg aagtcaacct caaaggcacg taccaccttt tgcgacttga
     1621 tcaactcccg cagttcatca cggcgattga aatcaacgta cagttcggcg gtgcaatgga
     1681 cgctgttcag aaactcctcg ggtgattcat aacccgccat tctggcgagg gcgggattca
     1741 cggcgagaaa ctctccatct ggtgtggttt gaaaaatgcc aaggatcgcg ttgtcaaaaa
     1801 tgctgcggta tttctgttgc gcgttctgca gcgcctgctc tgccagtctc cgttgagaaa
     1861 cgtcctgttt gatggcgacg taatggctga tcgggcctga ggtggagcgg actggggcga
     1921 tcgtcatgtc ctcggtgtag agggtcccat ctttcttgcg attgatgatt tctccccgcc
     1981 aatggctccc tgccgcgatt gtgttccaca aagcgccata gaatgcggca tcttgtttac
     2041 ctgactgcaa cagcttggga tttcgtccaa tcacctcgtc cgcacggtaa cccgtcatct
     2101 gagtgaaagc ggggttcacc cagagtattg cgcctttctc gtcggtcagc acgatcgcat
     2161 tggcggcttg ttccagggcg gtggcttgca gacgcaaccg gtcctccgct tgcttgcgtt
     2221 catcaatgtc catcgcgatt cccgagatcg tgagcagact cccgccctcg tcgaagaagc
     2281 cccatccctt gatccaaatc cagtggaccg tgccatcgcg ccagcacgcg cggtgctcgt
     2341 gagaaaattc gggtttctga ggtgtgacac tcgttataag ccttctcaaa gactcgcgat
     2401 cttcgcgatg tacggtatcc atcaagactg tgaagtccgc gggcgactcg gggctcagcc
     2461 ccagtaatcg cttggcagtg gcggaccact cctgcttatc atgggtgacg tcccacctga
     2521 gaaagccgat tcgtgcggct tcgatcgcag ctcgcatgag gtcttcatct tggggaaacc
     2581 cgatttcaac ctgcccctgc gcgctggttt cgcagcgaga ctccccaccg ctaccccgac
     2641 gttgttcaac ctccgcgtcc atcgctcgac gagagtttgc tccccgttcg gcgacaactg
     2701 aactgagcgc tatccccacg atcgtgaacc ctagcgcgat caattgcgag gcgcggatgg
     2761 tcatagagag gtggggccac tgtttggtca attcaaagac gagcatcaga caggcagcga
     2821 ccaagccagt tgtgatcaat ccagccccga gactatttga caggcgcagt ctctgatcgg
     2881 ccgactggtt tgcagcctcg ccgttggacg ctactttttc aagggctgct acgttgcggc
     2941 ggctgtcttg cgaccgcctg agtgtctcat tctgcgtctc tccgatctgt tcggaagacg
     3001 actgcggtct ggaaaggctg caagtgccgg caggtgcgtt tccgaccgga gcattgcccg
     3061 cgcaggcgtg gatcccccga atgctgtgca agtcagcctg aggtatctcg gaacttcgga
     3121 gagccgaatc gaggctcgtt accatcaggt gcaatgcttc agcggcgtac gtttctgacc
     3181 cggcatatgc tcgtccggaa gcattcctct attatggtat cggccaaatc gccctttttg
     3241 ttaatgtctt ctttcgctat gacgttatga tggaagttaa cgaattggtc gatattgggt
     3301 acgaggaaca gcaataacac tcgtggcggc gactccgttg gttgaaagtc gcggatgccc
     3361 aacgatgaag tgcggcatat cagtaacggc tggaagccgc ctgcgtgagg ctcatcgttg
     3421 gagtggcgag ctcggccatt ttgttcgttc tccctcgcga ttgcacctgt cgtcgtcgtg
     3481 gtgagactag agctcgcgaa atccacaggg agataattca tggaactcgg ttcgcgtatc
     3541 gacggctcta atgggttcag cgcgcctatc gatgacgatt cgtcttcatt tcacgcgaaa
     3601 ttgttggaca gcctcttcga cggcgtttat ttcgtggatg ccgagcgcaa gatcacttat
     3661 tggaatcagg gtgctgagag cttgacgggg tattcgagtc gtgaagcggt gggaagacac
     3721 tgttatgaca acttcctggt gcacattgat gagacgggcg gcgctctttg tacaatcggt
     3781 tgcccgctta cgagtacgat ccacgatgga caacgaagag aggccgatgt cttcctcaga
     3841 cataagctgg gacacagagt acctgtatgc gtaagggtgg ctccgataac cgaccctgca
     3901 cgacaaatcg tcggcgccgt cgaggtgttc agcgatattt ccgccaagaa gcaggtcgaa
     3961 aggcgagttc acgagctcga aggcatggca ttccgcgacg gtttgaccgg ccttccgaat
     4021 cggagctaca tcgagttgaa agcgaagcaa tcgttcgaag ccttccaaca gttccaacga
     4081 aacttcggtg tgatgatgtt tgatgtagat cacttcaagc aagtgaacga ccgccatgga
     4141 cacgatgccg gcgatctcgt gctgaaagct atttctgaca ctctggcgaa aggccttagg
     4201 gaaaacgaca ttgtgggccg ctggggcggt gaagagttcc tggcgcttct cgtcgacgtg
     4261 gatgcttgtg tgcttcgcca actagccgag cgatgccgga gtctggtcgc tgggtcggga
     4321 gccttaagcg gcgactcgcg gatcactgcg accgtctccg ttggcgctac gctctttacc
     4381 caagcagatt ccatacagtc agcaatcgcc cggtccgatc aattgatgta tgttagcaaa
     4441 tcgagtggta ggaacaaaat cacgataggg tgaatggcac gccactccga caatcaaggc
     4501 accggcagca tgctcattcg tatcaggcat tcccgccttg gaagagcagg tctacttcgc
     4561 cggtaccatg gcagcgttag ctcgatttct ttcaggattc ttggctcgac tatcctctgc
     4621 cgcatccacc aggacacatc ctgtgcagac tcgtgacagg cggacgattt ctagaccgag
     4681 caccatggac cgcttgccga gggtctgatt gtcgatgaaa cggtgcgctg tccgtggcac
     4741 cacgcctgtt tcagtctttg cactggggaa gcactgcgcg cgcgcctgcg ctagacccga
     4801 tcgcgtgtag gcgcgtcgag acggtcggcg acaagcctat ctaagagaag aactcactga
     4861 ttcccgagat ccaattctag agccgttccc ggggatgatg tatccgagtc atggaactgc
     4921 caccttgtat tatcgtgcac atgactcgca aacggcgtcg ctcttgtagg cgattacgat
     4981 cccggtgtca tcgcgcacca ggacaacgcc ccgggaggcc atacgtgctc aggatgcctt
     5041 ggcgagcgga ttgcgcctgc tggtcatcga taagtcgcgt attgcagttt cttcagatat
     5101 tgttagggtt gcccggcggt agtgccggtg agcgtcgctg cggcactggt gccggagacc
     5161 agtcgaaagg cacccttttt tagggtcagt caggattgat catgatcgag tacttgccgg
     5221 acggtagcat ccaaggtgtc cagggtgaaa ggcttctgga gacgtcgtcc ctgaggtacc
     5281 acttcgtctg acggttcttg cgtagtctcg ttgtagcctg atatctggac ggacttgatg
     5341 gtcgggcaga tttcccggat ttttcccgcc aactcctgtc ctcccatttt gggcatgatt
     5401 atgtcggtga ctagcaggtc gaagaatcct ggggcatcgg ctaccaactg cagggcgtct
     5461 gcgccatttc cagcttccac caccgtgtaa cccaggcttg caagatggcc accgatcagc
     5521 gatcgaacat ctttctcatc ttccgccagc aatatcgttt ccgtgccgcc ttgccgtgcc
     5581 cgatgttccg ggaaataaaa gcaacgggga gtatcgtgag aagtcgaaag tctaggcgag
     5641 cagctcagcc agcgaggagg cgtcgctatg attgaggtcg tcattgctga ccatcaggac
     5701 atgtttcgca ccgggataga ggaggtgctg aaggtcgagg acgggcttcg gatggtaggc
     5761 gaagcaccgt gtcccgaaca gttgctgaac actctgcaaa aggtcagtcc tcacgtcctg
     5821 cttctgtcaa agagcttcct accagcagtt acaagaatca acccgatgtt ggagcggaat
     5881 aagactgcac tgctgttgtt ggccgaggac aatgatacaa agacggcgtg gagcatctgc
     5941 tggcgatggc gctggagaat ttgcgaggcg acaggtagct gtatcgacaa agaaccgagg
     6001 acgaggtcac ctgtttgcgg attgcgtccg ttggtggaca cccagcaact tctactgcct
     6061 cctcacatga cgcgaatggc tgcaccccac cttctcatgg ccgaccttct catttaccca
     6121 acgcaccttc tcccctaacg ccatgtaccg cttctcccac ccacaaaaga gaaggaatgc
     6181 gtcgggctgc tcgctctctt gtggcctttc ctgtgacgac ctatctttga gtttcacaga
     6241 aagaatgagt aagacaagga aaggaatcat caaatgcact tatttcttaa actcggtatc
     6301 gcgctcgcca tcctcgcatt cgcaacctcg tctttcgcag caccaactac tttcgactgg
     6361 tacttcgcaa gcacagcggg gcatatctct gggaacggag cactgactgc ggtacctgat
     6421 ccatcggcta ctgctggctt gtacgacatt acaggcgggg ctggaaccgt gacctctcac
     6481 tcagtggcat tcgcggtcac gattgcgccg tgccttaatt atgcggccac atgcacgttc
     6541 gtgaataccg atggccatgg cgccaatctc caagttgata atttgttgta cgccaaccac
     6601 gctccgggtt cctacttaga tgggtacggc attgtcttaa ctcccgctcc tctaggttcg
     6661 ggtgcatcgt atatcggcat ttgggatagc ccttcgcagt atttttacaa ctacactgcg
     6721 gacggctata acaatctatc gactccattc accattgaat cgagcgcaac tcctgaaccg
     6781 agcaccctgt tgatgatggg ttcaggcatt ctcggcttag ctggactggc gcggaagcgc
     6841 ctgtttagct aaataatccc ttccacagcc ccagtgggaa tggcgctctg ccatgcctgc
     6901 tgggggtttt gagggagcat gtacttgtga gcctactgag ctgctcacta gccgatgaaa
     6961 tggccgtctg gccggacgag caacaaagga gtttacgagt ggcacaccag agttctagaa
     7021 aagtcctcag acccaagcgc acccaagatt tccagaggtg gaagggtgta ccgatccgat
     7081 tcagtagggc agaaatcagg ctgattacgg cagcagtcaa cgccagcaat gctcgctctc
     7141 gttaagcggg ggtgtagccg acaagtcgat gtctacctca atccaatgaa tactgcaggg
     7201 atgggctatg tgcgccatgt tagccgggag gaatatccaa caataatgct gctgacaacc
     7261 gtcagcagcc tattctgttt cgcatgggat cacttgggta tattcgttac gcatacaggt
     7321 actgacacaa ggtctccctg ctgatctata ataagtatgc cagcgttccc tacaacgtct
     7381 gaacggaaca caggggcgcg agggcttgtt ttgggcttca gttatcgtac tggactcagt
     7441 ggattgagac agacctcatg tttgctttgg ttattcgacg gtcacatcgg cagcagggcg
     7501 tgttccgcca acccatatgc acgagcgatc tggttagctc tggcgcgtga gtgcgcttcc
     7561 gcaactaccc gattcccgtc tggataattg atgatcacac aaaacccctt gccgcaagca
     7621 gccgtcgcgg gcgcgacgga aacacccgtc gaataacagg tcttatgcat caacctaaca
     7681 ggcatgccat gcctgctctc ggaatcgctc ctgccactgt cggtgtcggc ctctgtcgtg
     7741 gcgagaagcg caggttcggc gagttcaggt caacaaacct gtgcttacat cgtttgcagt
     7801 accaactctc cattctgaag cagcatccgc ccataacaca atcctcgcgc ctcaccctct
     7861 ccttcccact ttcgtccaga ttattaagcc cgtacacgat gggcacgacg gtggcgttct
     7921 gctggcacat ggagcatgaa gggccaggag tcgtggcgct attcatcgaa tcttaccgtt
     7981 ctttgccctt cgtattttcg gtggacatcg gcacctccac atgaaagatg gctgcgactc
     8041 cgaaaccgct agttgggtac gggtcccgct gcacgtggcg aacggaaccc atgccctcaa
     8101 gctggagcgt tatgtttggc ggtgattgcg cggatagcgt tacttgtaga gttacagtcg
     8161 aagcgactgg cggtatggag tcgctctcga tgaaaactcc accttttccg atatcgcgtg
     8221 taagtccgat gccgtcatac cactgaccat cgactgcttg ccatctaaac tgaaccatgc
     8281 cagtgatcag gtaccgagca ctcattcgtt tgtcttctgg gtcggtacct gtggtcggtg
     8341 ctggtttgac tcattcatgt cgttctcaat gcgttctttg aagctcaaac ttatgccgac
     8401 agagaattag ccacaagaga gcgaacagcc caacgcgttc cttctctctt ttggaagggc
     8461 gaaacggtac catttgttag agaagaaggc ctgggacata ttcctcgccc gaactgcgtg
     8521 cgaggagcct tttatatgca cgctaattgc cgcgccaact ccgacataag tcgtggcaaa
     8581 tcagtcattc ttcagtgagg tcgtccaagc taagtgaaaa gagttgcaca ccacagtgct
     8641 ggttagcgcc cttactcttc ccaggataga actgtaaagt gttctgccac gaacaaggcg
     8701 cggtatcaag agtcggtggt ccaacccgcg ctgctgttca tcaacagttt tgccccgcaa
     8761 ttctacaagc tgagcccgtt ctttcccgcc gacgcccgac cttcatgcgg ttcgctgtgt
     8821 cgcatatacc gcgacattcg gttcgccgcc gacaagcggc ccttcaagac gcatgtcggc
     8881 gttcattttc atcacgccag cggcaaggat gcccacgcgc ctgtgtttta ccttcacttc
     8941 aaacccgaaa attgcttcgt cgctgcggga atctggcacg agataatccc accctaaccc
     9001 gagtgcgaac ggccatggtg caggagcccg agcgatgggc agcgatccgc aagaaactgg
     9061 agtggaaggc gacagtctgg cgagaccgcc tcgcggattc gattgcggcc atcgctttat
     9121 cgatgacctc aagatgaagg ccttcgtgac ctcgattgca tacacgagga acaggtttgt
     9181 ggtcagaagt tgctgcgcga tttcgaacgt gcctgtcgga aaatgtcgcc gttggttgag
     9241 ttcatcacga cggcgttggg gcagaaatat tgacgggagg agggggcatg ctggacacca
     9301 cactgttggc gtcaatcgcg cagcagatga tgagcagcaa caccgttggc aggtcactct
     9361 aactcagcgg agacgacgta ctcgcccgac tcactgtccc aatcaatgcg gagcacattc
     9421 ggcttgcagc agacctggca gtcttccacg tagctctggc gcgttcccac agaagcatcc
     9481 accaccgtct cgttccaccc gccacatccc gcacactgga agcctgaggt catcgattcg
     9541 ggattatggt acaacggaac gaaaccgtct tccgctccca cagagtcggg gcggctggca
     9601 tgccgcagat tgcttgatcc gcaccttttc cgctctggtg cgggtgggaa agtccactgt
     9661 cacatacagg gaggctcagg acgttgatca gttaacttcc gaggagtgat cggacagtgt
     9721 gagatcaggt ggcctctgac gttcgcgggt tcgcctgtca gagatgctgc tgtcgcccca
     9781 cgcccatccg cactcgcaat tcaggatttc cttcaggtgc tcttttatca ggactcgatg
     9841 acaatgaggg caggtgcgat accacataga ttggctactc tggttcttta ccgaagaagt
     9901 cgatggtcga gaactcagtc ttattatcgg caatctggac gaaatcctta actcgtgggg
     9961 agcatttggg ggtaactggc taagagccag tgatgctccg ccgttccgaa ctttgatttc
    10021 accagattgc agggtttggg caatttacgc gatcacgaac agaaaaatac tcttgaccca
    10081 gtcgccgctt ctggggagag gatgcgctta tgcgagttcc ttcacgcccc gtctccttgg
    10141 ccagttgctc tctggtcgcc ttgggatgcc gcaagccctt ggagcagaat tcgcacatac
    10201 ccagcttgtg aacgggctca tttggccgtc cgcgctcgcc atacgtctcg caagagctag
    10261 tactcatggc tttggatgtc ctcggcgcat ccattgacgg ctccgcagca atagtcgcgg
    10321 ggtcgctctc tcgattaaga gaacgacccg acccgtattg ccaactggct tgctctcggc
    10381 gcgaggtaag tcagggacat gtgtcaacag caatacggcg ttccaagtca aattccctga
    10441 actcgacggt aaacgccatc tttactcggg ggaatatggc gtatctccag atttacgcct
    10501 cccgtgatcg aaagcaagtg agaacgtgca gagctttatt acgaaagtaa ccaccatgct
    10561 tcctgttttg cacggttcgc aggaagggct gcttcgcgcc cacgggagtg accgccaacg
    10621 gatcagtgat tcacggcttc acgtggtcgc cctcgcctca tgcatttaat cgtcgcaagc
    10681 ttcccttttt ggaacggaca ggcttcttac ataaacctgc ttcgaaaggg gtgccgtttt
    10741 ataatgcgtg gcccgggcaa taacgtaccg aacttccgca aaaattcggg tcgtgaacct
    10801 cgacgtagca atcttggacg gctgaattcg gacccgacgc ggcgttttgt ggttccgcaa
    10861 agcagtccta cacttacccc gcagcatggc tcctgtgtcc tccaacccgc ctatttcgtt
    10921 gtgccgccag caagtggtca gcgtccagct gcaggaagat cctgctgcca gtgtgttcgc
    10981 caatcaaacc aagcttctct tgtcgcggcg gcgcgctaag aacacgaaga cgccggacta
    11041 ccgagaactg aaaaacaaga gcccgctaca cagagcattg aggccgaacg cgatgcaggc
    11101 tgcaattgtg acacccttgg cgagctcgga gcgattcctc cggcgcagcc acacgatcag
    11161 cggaatcaaa tagagccact gccagagtcc aatctggaaa agcgcgatga gtccggccgt
    11221 gctcagcctc ggatatacag tcgacgcaag gacgcacaga acaacaacaa ctacgagggc
    11281 gatgaatgtt gccgccaacc acgccagaac gccgccggaa actgtgtcgt tattgaacat
    11341 gttctgctcc cacgcccgca cgcaaggcgg ccaggcgagt gtgctcaagc tcatcgcggt
    11401 aaaagaggtt gtaattatcg aaggggataa gacggccgtc ggggtgcaca atgtgcacgc
    11461 aggtcttctt gatggagcgc acgtcgaacg agtaggcgtc gatgaactgc atgatgatca
    11521 cgcgaaagac attatcgtaa ctcagcgaag gcgcactgac gcgtggcagg cagcacagca
    11581 gatcgtgcag ggttcccgct ccgctcttag gcgagtgact cgtcgagaac agccggaaaa
    11641 tatgatcgtg aagactttgc tcctgctcgt aaagcacggt attgtggcca ccttcaatca
    11701 acacttcggg cgggatgagg ctggtcagcg gcgtgatctt gccgtcgagc ttgagggcgt
    11761 atgccatggc gaggctgtcg ggatggcacg gcaccggtac gatgtcttcc ggccggaaaa
    11821 cgctggtctg ttcgtaaatg cgccggcgga cctcggtgag tgtgagccgg tcagcggccg
    11881 ggttaaactt ctcaagtcga ccagcgacct ggatgggctg aaacgtcacg ccgcgcacac
    11941 aaggctgctg caatgcgaaa tcgataatgc ggccgatctc atcatcgttg agtccacgtt
    12001 gcagggtgac gaccagcgtg gtggagattc ccagccggtt aaggcgttcg agagcacgcc
    12061 ggcgcacgtt gcgcaggtcg gcgccgcgca aggcattcaa cggtccggcg ttcagcgaat
    12121 cgaactgcag gtagatttcg aagtcgggca tatacccggc gagccgttcg gcgaactctt
    12181 cttcagcggc gagtcgcagc ccgttggtgt tcaccatcag gtggcggatc ggccgtcgct
    12241 tggcggcgtc gagaatggca aagaaatcag gatgcatcgt cggctcgccg cctgaaatct
    12301 gcacgatgtc cggctgctgc tcattgcgaa ccacagcgtc cagcatttgt tccacatgct
    12361 ccagcgtgcg atagtacggg cgctcgggcc cgctgacgga atagcacgtc ggacacctca
    12421 ggttgcagtg atcggtgatc tcgaccagcg ccaggcacga atgctgctcg tggtctgggc
    12481 acagtccgca atcgtatgga cacccccaac gaaccggcgt gttatacacg gcggcctgct
    12541 cgggcggctt aagaaacact tcgcggcact tccggtagta atcgacgtca tcggccatca
    12601 tcaccgtttc aatgccgtgc acgggacagc gtttgagcat gagcactttg ccgtcgcaaa
    12661 agacgatctt gccttcgacc ttgcgaaagc agcgcgagca gatggagatg gcaacgtcgt
    12721 aaaaaagata cgggcggacg cgatcgttca tgacgtctcc ggcaaccgat gaggaacgcg
    12781 gcggatgatg tcgagcactg tgggcgcgca ccataacgct cccgcaagac acgcccattg
    12841 cagggcatca aggcccagca gacggggact gtcctgcatg aactcaaggc cgaagcgcaa
    12901 tgaaaagtag ctaaacacaa acaggcggaa acggtccccg ttcacgggag gccacgcgag
    12961 ctcacgcgag agcagaatat acgcgagtag aaagagaatc tcgtacagtt gcaaaggatg
    13021 ccgcgcaaga ccgtcaccga aatcgatacc ccatggcagc gtggttgggc gtccgaacgt
    13081 atcgtcggcc gtgccggcaa agaagcaacc gatacggccg aggatcagcc cgacgatcag
    13141 aggagtcaca aaaagatcgc cagtccggct ctgaatgcgg tacttgtgtt ttacggcttc
    13201 aaccgcaatc cagccgccga ggaccccgcc cacgatggtt ctgcccccgt cgagcaggaa
    13261 caaggcatgc cagcggaaga tctggggatg ttccagacac gccagaaggc gggaccccaa
    13321 cgcggcgccc acgaaggccg cacaaacgat cgtgctgcgc aggctgtcag gcaggaagtc
    13381 accgctcctc tgacgtgcgc gtatgtacag aagcgaaccg gcgaggtaac ccaacagctc
    13441 gaaaatcacg tgcagggggc ctcgcgccat cgacgggatg aaaatcatgg acacggtatc
    13501 gagagcttag ccgagtcttc cgactagaac aaggacagat gtgctgagat cttcatatat
    13561 ttacctaccc ttagacgaat tcgagtcagg gatgtgaacg taaggaaatg ccgtgtcaca
    13621 acttcacagg acgatggttg ttgagaacag gtgcggcatg acggttcccg tcaccattgg
    13681 tccaccgcac acggggcaga tccataaagt aagagcacga accgcatcca atccccatag
    13741 ccacgcgcta gcggctcact gcaaattgcc ctatcgacaa tgctccgccc aactctatct
    13801 agatcaccca agtcttgtcc gcggagcacg gtcgatacgg cactacgcat ctatccctac
    13861 taatctcgca acctacgcat ccggcctgcg cacaatcacc gaattctcgt tacttcccca
    13921 gactccagcg agtagtaggc ctcgataatg gtcagcttgt gctcttcgga agccttgcta
    13981 agaacctcgc tgcgcgacag cgcctcctgt gcgacctggt gcacatgatc acggattgcg
    14041 atatcgagcg tctcgggctg cttcggatcg accttcatac aagaggggac gatgggcttc
    14101 aagaccgcat cgaggttaac ggactccgat ttcccgcgtg aacaggcggc tgtcactgcg
    14161 ccgcaacttt gatgtcccat gacgacaatc atgctgatgc ccaggtgctc aacagcatat
    14221 tccaagctgc cgagttcaat agggccgtca tcttccccag ccgagcggac gacgaatagc
    14281 tcgcccagtc cggcgtcaaa aacgagttct ggaggaaccc ggctatccga gcaacccaac
    14341 acggcgacga gcgggtgttg agtctttgtc aaaaacttcc gctgttcccg cagatcgtgg
    14401 tgagccatct tccctgtcac aaagcgccgg ttaccagtcg caagctcacg ccaaatgtta
    14461 tccgctgaaa gtgaggtgga gtcttgggat acagcagcaa tacacagtag aaccagcact
    14521 ccgatagacg cagacatcgg cttcacccaa accataactc ctccctttcg tcgcaaaact
    14581 ttactcttag taaatctcag gcgtggctta tggctggatc cgtgtcgttt cacgttcgac
    14641 aacggcagcc aacctaattc atgtctacat gtaacttgga tctgtgtctt tcctccacat
    14701 tttgacttcg caaagattcc ggcgaccgcc gatgggaaag atttgtggcg ctgctgtgcc
    14761 aataggaagc cggcccaaaa tggagttcga tgagaccgtc gtccgaggcg cgttcgggat
    14821 tcagcagtcc gtgggagcct tggcgaattc cgacgacctt gcagccaagt cctcggccgc
    14881 cgtaccgtga attaccgttg agccccgacg catcagcgct gtcagtattg atggcgatcc
    14941 tctttcttgt ggcttccgac atcgtatcga cggacctgcc ttcatctaca cctatcgtgg
    15001 atcgggtcgg atccgaacga tccttggata ccctactcag aagatcgcgc aagcccgata
    15061 attcgaaaag ttaaaatata tttggttagc cttcattccg attcgttgct gaaaaggcat
    15121 gttacttca
//
LOCUS       DASRKK010000038         9692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1272588, whole genome shotgun sequence.
ACCESSION   DASRKK010000038 DASRKK010000000
VERSION     DASRKK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272588"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /gene="lptD"
                     /locus_tag="VFC15_00775"
     CDS             complement(<1..2000)
                     /gene="lptD"
                     /locus_tag="VFC15_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZM08725.1"
                     /translation="MISRTRLIITTLLGCHLILAPALLTSQLRSASEDDQSAQTASPQ
                     TASPDAPARQTPTSKPGPPPPAEEDEGMRVMEEVVQQQLQSAAEQDSANPRPPANVVL
                     GRDEVLIRADEQEKSQDIYKVRGNVEIRFRDNTLHCDQATYDSTTGQITATGHVVYDG
                     GAHNEHLVGTHATYDVSRDTGTFYDVTGSTGIKVSNQSMFLKSSTPFFFTGKVVDKLG
                     PDLYRVHQGIITSCQLPKPKWEFHPATATVEMGGDAKMYHSTLRIKGVPIFYFPYVQH
                     PADNLGRKSGFLIPAIGQSNNKGTILGDSFYWAINRNSDAEIGGYYFSARGWAQTGNY
                     RNIGWRYQFHAEYYGVIDEQGNPVSKQNQGGEELRVNGSVVLPYGFRGVISVDYLSSY
                     VFRLAFAQNFAEAINSEVRSAGFVSKSWSGNYVGLLMSRYQNYQSTVNGDVIDIAHIP
                     SFQMAGVENSILNSKFMYTYDVAAEGVSRHEPGFQTQNVVGRLDASPTISLPTFLQGW
                     TFRPEVGARETLYTERLLPTNGNTGVIGVAVTQAINRNVLLASMEMRPPSIAKIFDRK
                     LFGLVMKHVVEPYAIYSYETGINNFANIIRFDERDILADSSAVEYGVVNRLYAKKSKS
                     SGECYDHPKYLPGDTPPDEVAKKLAADSKLCDDQKGPAREIVT"
     gene            complement(1997..2314)
                     /locus_tag="VFC15_00780"
     CDS             complement(1997..2314)
                     /locus_tag="VFC15_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08726.1"
                     /translation="MSWRDYSFAMRAAAACFRNTVLYRRTAPKRKSKVVRSSIGKAAR
                     LCLGSSDINGATGMWQAGLEKHQSGVKLSKAFRVLPQSVQRAKLQGCAESNAFGEGTR
                     GFR"
     gene            2313..3368
                     /locus_tag="VFC15_00785"
     CDS             2313..3368
                     /locus_tag="VFC15_00785"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZM08727.1"
                     /translation="MTEQQLSQTAAEDISPDPAAELWRSEVQSRLAGYQKRRGRRVAG
                     AFSMRFPFPADEVAEPVETAAAVPSISEMPEVFPEEGLTSQAAEEYLQIDEPVPTVES
                     ELTEEPVAEENESVASGSVDLVLEPTPEPGPEPFVDTVPRPRPKRKVIAFPRHLSVAP
                     ETFYRLADPVTSESPRILDVPEELEAIPTTPFLDGLQLDQVNSADAARDREHVELPFR
                     AVRVSQRVFAGVVDVLLVGVGGAVFAGVAWKVLAKPPLTKPLILGMVVAAGLLWSVYQ
                     YLFVVYAGKTLGMMAAKVRLRTFTGRTPNLRQRRHRVLGFYLSALSLGMGLMWVFVDV
                     DALCWHDRLSRTYLADR"
     gene            3450..4961
                     /gene="gltX"
                     /locus_tag="VFC15_00790"
     CDS             3450..4961
                     /gene="gltX"
                     /locus_tag="VFC15_00790"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZM08728.1"
                     /translation="MSKDSKVRVRFAPSPTGQLHVGNARTALYNWLFARKTGGTMILR
                     IEDTDLERSESRYEDQLLDDLKWFGVDWDEGPDVGGPHAPYRQIDRLAIYREHAERLV
                     KEGKAYLCFCSQEELERDREAAMAEQRSPLYSGNCRGLDPAESERRRAGGEAAAIRLA
                     IPEHPIRFHDIVRGMVEFSNEVVSDPIILRSSGIPVYNYVVVVDDALMDITHVIRGDD
                     HLSNTPKQVALYEALGWPVPEFAHLSTIVGSDGARLSKRHGATSIANFREMGVLPEAL
                     VNYLALLGWAPTGGNREVFTPTDLTKEFTLERVTPSAAMFDMEKLYWLNRHYIKNSPP
                     ERIEKLAESYFVKAGLLPENPDERTRAWFSKVVALLVPSVNKLDELTERAGLIFHVDA
                     AAALSAADNAEVLGSPKANEVLAAFAEQVESDPAPMTPERFKAIMNEVKAGTGTKGKD
                     LYHPVRIVFTGSHSGPEFDKLIPILEEGSQLTLPVHVMNTQERLAAFKAARAV"
     gene            complement(4969..5658)
                     /locus_tag="VFC15_00795"
     CDS             complement(4969..5658)
                     /locus_tag="VFC15_00795"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZM08729.1"
                     /translation="MPKHIGIVACSAEGAALCYRTICDEASALMGRHMHPEISLHTHC
                     LGEYMGPIRRFDWQGVADLMLSSANKLAAAGADFLISPDNTIHHAFDLVAPRSPLPWL
                     HIVDEVAVVAKSHGYHKLAVTGTKYTMEGTVYQSKLHAAGFEYLVPDGDERAKIDQII
                     MDELVNGSFTAESRAYFRQVIGRMSHQGCDAVVLGCTEIPLLIDDVTSPLPTLDSTRI
                     LARAALRKALE"
     gene            complement(5789..6838)
                     /gene="add"
                     /locus_tag="VFC15_00800"
     CDS             complement(5789..6838)
                     /gene="add"
                     /locus_tag="VFC15_00800"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZM08730.1"
                     /translation="MPNDAPSAFIVSLPKAELHLHLEGTVDPATLAELSRRHPTPLST
                     ENNRYTNIADSGRVFTEEEAHALYQYKNFTGFLLAFKAVTERLRTPDDYELVTYRMMQ
                     KLHAQNVLHAEVYVSVGVIRWRGGDFAPLFEGLERGRKRGERDFGMSLYWIFDAVRHF
                     GPRRAGLVLDEAIALRKTSDAIVGIGLGGDERRAAPELFRDVYARAATHGLRLTVHAG
                     ETAGPESIWAALRELKADRIGHGLHATDDPDLVRFLAEHQVPVEACITSNALTGCCAA
                     VEQHPIRRLFDAGILVTLNTDDPDMFRTSLAREYQIAQEVFGFNEAELRQLAENSFNA
                     SFLPEEKKREYLAKF"
     gene            complement(6945..8534)
                     /locus_tag="VFC15_00805"
     CDS             complement(6945..8534)
                     /locus_tag="VFC15_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM08731.1"
                     /translation="MDSSIPGSTVLATQLFACKSIDKLISESEEPEHRLKKTLGPWSL
                     TALGIGAVIGSGIFTVIGTAIAGQKFDTSSILNAPLADFIVHHMATLGRPGAGPALAV
                     SLVLVAIVCAFTALCYAELASMIPIAGSAYTYTYATMGELIAWIIGWDLILEYAVSNM
                     AVSVGFAAHLVDMFDWFGWHPALRWISPAYLPSGMTDLKGDMLYAPGWHFGFNFPAFL
                     IVMLLTVVLVRGIRESAETNNVMVILKIAAILAFVFAGMHYIHPANYHPFAPNGWSGI
                     LTGGSIIFFTYIGFDSVSTAAEEAKNPQRDLPIGIIATLIVCTVLYISVAVVLTGIVP
                     WQRLIDDAAPVVNSLKQLGITTGSHSLHWIRLAVLFGAMMGMISSLLVFQLGQARVWF
                     AMSRDGLLPKLFGRVHPRFRTPSTATWIAGFLVGIPGGILDIGTLADLSNIGTLFAFA
                     LVSIGVIILRYKQPERRRGFRAPGGIAAPILSVLFCLLLMSGLPILTWIRFFVWLAIG
                     LTVYMLYSRYRSEFCPEDRRR"
     gene            complement(8592..>9692)
                     /locus_tag="VFC15_00810"
     CDS             complement(8592..>9692)
                     /locus_tag="VFC15_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM08732.1"
                     /translation="YLGAAADAGTTVIDLSGKTVLPGLIDAHTHLTFNPRFGYDTLAI
                     SVPREALIGAKNARVTLLAGFTSVRNVGANAFTDVALRDAINAGDVPCPRIDASGPAL
                     GITGGHCDNNLLPYEFHATEIGVADGVENVQHKVREVIKYGADVIKVCATGGVLSLGD
                     DPQHSQYTQAELAAIVADAHRLGRKVAAHAHGAEGIRWAAEAGIDSIEHGSYIDDAAI
                     AAMKQHGTYLVPTLYLGDWFLENAPKTGVPPQMLAKAQAVLPAARKNVAHAFASGVKV
                     GFGTDAAVYPHGLNAHEFAVMVKLGLTPLQSIQAATVNDADLLGWSEKVGVIEPNHFA
                     DIIAVDGDPLQDVTTLERVKFVMKGGVVYKNQ"
BASE COUNT         1978 a         2762 c         3033 g         1919 t
ORIGIN      
        1 gtaacgatct cgcgggccgg acccttctgg tcgtcacaga gcttgctgtc ggcggcgagt
       61 ttcttggcga cctcgtcggg tggagtgtcg cccggcagat acttcgggtg gtcatagcac
      121 tccccgctgg acttcgactt cttggcatag aggcggttga cgacaccgta ttcgaccgca
      181 ctggaatcgg ccaaaatgtc gcgttcatca aagcggatga tgttggcgaa attgttgatg
      241 ccggtttcat aactgtagat tgcataaggc tcgaccacat gcttcatgac aaggccgaag
      301 agtttgcggt cgaaaatttt ggcgatggac gggggccgca tttccattga ggcgagcaat
      361 acgttgcggt tgatggcctg ggtaacggcg acgccgatca ctccggtatt tccattggtg
      421 ggaaggaggc gctcggtgta gagggtctct cgcgcaccga cttcgggacg gaaggtccat
      481 ccctggagaa aggtgggtag agaaatggtg gggctagcgt cgagacgccc cacgacgttc
      541 tgagtctgga agccgggttc atgccgcgaa acgccttcag cagcgacgtc atacgtgtac
      601 atgaacttgg agttcaagat tgagttctca actcctgcca tctgaaaact cggtatgtga
      661 gcgatgtcga ttacgtcgcc gttcaccgtg ctctgatagt tctggtagcg ggacatcagg
      721 aggcccacgt aattccccga ccagctcttg ctgacaaaac cggcggaacg aacctcagag
      781 ttaatggctt cggcgaagtt ctgggcgaag gccaggcgga agacatagga gctgaggtag
      841 tcaacggaaa tcacgccgcg aaaaccgtag ggaagaacga ccgagccgtt caccctaagt
      901 tcctcgccgc cctggttctg cttggaaacg gggttgccct gctcgtcaat gaccccgtaa
      961 tactcagcat gaaactgata acgccaaccg atgttgcggt agttgccagt ttgtgcccaa
     1021 ccgcgtgcag agaaatagta gccgccgatc tcggcgtcgg agttgcggtt gatggcccaa
     1081 tagaaagaat ctccgagtat cgttcctttg ttattcgact gcccaatggc tgggatgagg
     1141 aagccgctct tacggccgag attatcagcc ggatgttgca cgtagggaaa gtagaagatc
     1201 gggaccccct tgatgcgcag ggtggagtga tacatcttgg cgtcgcctcc catctccacc
     1261 gtggcggtag cgggatggaa ctcccatttg ggcttgggaa gctggcacga cgtgatgatg
     1321 ccctggtgca cccggtagag gtcaggcccc agcttgtcca cgactttgcc ggtgaagaag
     1381 aagggtgtgg aggacttcag gaacatggat tggttcgaca ccttgatacc agtggagccg
     1441 gttacatcgt aaaacgtgcc agtatcgcgg ctgacatcgt aggtggcgtg ggtgccgacc
     1501 aggtgctcat tgtgagcgcc gccgtcgtag acaacatgtc cggtagcagt gatttgcccg
     1561 gtggtggagt cgtaggtcgc ctgatcgcag tgcaaggtgt tgtcgcgaaa ccgaatttcg
     1621 acgttgccgc gaactttgta gatgtcctgg ctcttttcct gctcgtcagc gcgaatcaag
     1681 acctcgtcgc ggcccaaaac gacgttggcg ggcgggcgcg gattggcgga gtcctgttcc
     1741 gctgcgctct gcaactgttg ctgcactact tcttccatga ctcgcatgcc ctcgtcttcc
     1801 tcggcaggcg gggggggccc gggtttcgac gtgggagtct gccgggctgg cgcgtcagga
     1861 gacgcggttt gcggcgaggc ggtttgcgcg gattggtcgt cctcggaagc gctgcgcaac
     1921 tggctggtta acagcgccgg cgcgagaatt agatgacaac ccaacagggt cgtgataatc
     1981 aatcgagtgc gggagatcat cgaaagccgc gcgttccctc gccgaaggcg tttgactcgg
     2041 cacaaccttg caacttagca cgttgcaccg actgaggcaa aacgcgaaac gcttttgata
     2101 gtttcacgcc gctttggtgt ttttctaaac ccgcttgcca catgccggtt gcgccgttga
     2161 tgtctgacga gccaagacac aaccttgccg ccttgccgat ggatgagcgg acgaccttac
     2221 tttttcgctt tggtgcagta cgtcgataca gaacggtgtt acggaaacat gcggccgcag
     2281 cgcgcatggc aaaggaatag tcgcgccacg acatgactga acagcaactt tcgcaaactg
     2341 ccgcagaaga tatttcaccc gacccagccg ccgagctatg gcgaagtgaa gtgcaatcgc
     2401 gccttgccgg ctaccaaaag cgccgcgggc ggagagtcgc gggagcgttc tccatgcgct
     2461 tcccttttcc agccgacgag gttgccgagc cggtcgagac ggcagcagca gttccgtcaa
     2521 tttcagagat gccggaggtt tttcctgaag aaggcttgac ctctcaggca gctgaagaat
     2581 acctgcaaat cgacgaacca gttcccacag tcgagagtga actgacggag gagccggtcg
     2641 ccgaggagaa tgaaagcgtc gcgtcgggaa gtgtcgatct ggtgctcgaa cccactcccg
     2701 agccggggcc tgaaccgttt gtggacaccg tgccgcggcc acggccgaaa cgcaaagtca
     2761 tcgctttccc caggcatttg tcggttgcgc cggagacgtt ttaccgcctg gccgatcccg
     2821 tgacctcgga atcgcctcgc attctcgatg tacccgagga actggaggca attccgacca
     2881 ctccgttcct ggatggactg caactcgatc aagtcaactc tgccgacgcc gcgcgcgatc
     2941 gggaacacgt ggagcttcca ttccgcgcag tgcgggtttc gcagcgcgta ttcgcgggag
     3001 tggttgacgt tttgctggtt ggcgttggag gggcggtgtt tgccggtgtc gcctggaagg
     3061 tcctggccaa accgccgctt accaagccgc tgattctggg gatggtggtt gcggcggggc
     3121 tgctgtggag cgtttaccag tacctgttcg ttgtttacgc cggaaagaca ctcggcatga
     3181 tggcggcgaa ggtccgtctc cgcacgttca cggggagaac tccgaatcta aggcaacgtc
     3241 gccatcgagt gctgggcttc tatctctctg cgctgtcgct gggcatgggg ctgatgtggg
     3301 tgttcgtcga tgtggacgcg ctctgctggc atgaccgcct cagccggacc tacctcgcgg
     3361 accgctagca gcagtcccgg actggcccga tttgaaggcg acgctgacat cggatggctg
     3421 gcgctccccg ctcttgctaa catcagagtg tgagcaaaga ttccaaggta cgcgtccgtt
     3481 tcgctccttc tcccaccggg cagttgcacg ttggcaatgc ccgcaccgca ctttataact
     3541 ggttgttcgc ccgcaaaacc gggggcacga tgattctgcg catcgaagac accgacctcg
     3601 agcgcagcga atcgcgctat gaagatcaac tgctggacga cctgaagtgg tttggcgtcg
     3661 attgggatga gggccccgac gttggtggtc cgcacgcgcc gtatcgccag atcgaccgtc
     3721 tcgcgattta tcgcgaacat gccgagcggc tggtgaaaga gggcaaagcg tacctctgct
     3781 tctgctcgca ggaggaactg gaacgcgatc gcgaagccgc gatggcggag caacggtctc
     3841 cgctgtactc ggggaattgc cgtgggctgg atccggcgga gtctgaacgc agacgcgcgg
     3901 ggggtgaggc cgctgcgata cgactcgcca ttccggaaca cccgattcgc tttcatgaca
     3961 tcgtgcgtgg aatggttgag ttctccaacg aagtagtgag cgatcccatc attctgcggt
     4021 cgagcggaat tcccgtttac aactatgtgg tcgtggtgga cgacgcgctg atggacatca
     4081 cccatgtgat tcgcggcgac gatcatcttt ccaacactcc caagcaggtt gcactctacg
     4141 aggcgctggg atggccggtg cccgagttcg cgcacctctc gaccattgtt ggcagcgacg
     4201 gtgcgcggct gtcaaagcgg cacggagcga cgtcgattgc caacttccgc gagatgggag
     4261 tgctgcccga ggccctggtg aattacctgg cgctgctggg ctgggcgcca acggggggga
     4321 accgggaagt cttcacacct acggatctga ccaaagaatt cacgctggag cgcgtcacgc
     4381 cgtcagccgc gatgttcgat atggagaagc tctattggct gaaccggcac tacatcaaga
     4441 acagtccgcc cgagcgcatc gaaaagctag cggaatcgta tttcgtcaaa gccgggctac
     4501 tgccggagaa tcccgatgag cgcactcgcg cgtggtttag caaagtggtt gcgctgctag
     4561 tgccctcggt gaacaagctc gatgagctga ccgaacgcgc cgggctcatc ttccatgtgg
     4621 atgcggcggc tgcgctcagc gcggcggata acgctgaagt gctgggtagt cccaaggcga
     4681 acgaggtgct ggcggcgttt gcggaacagg ttgagagcga tcctgcgccg atgactccgg
     4741 aacgattcaa ggccattatg aacgaagtga aggccgggac cggaaccaag ggcaaggacc
     4801 tgtatcatcc ggtgcgcatc gtgttcacgg ggtcgcactc agggccggag ttcgacaagc
     4861 tgatcccgat actggaagaa ggttcgcagc ttacgctgcc ggtgcacgtg atgaatacgc
     4921 aagagcggct agcggcgttc aaggcggcgc gggctgtctg aggcgactct actctagagc
     4981 tttcctcaag gccgcccgcg cgagaatgcg cgtcgagtcc agcgtcggta gcggtgacgt
     5041 gacgtcgtcg atcagcagcg gaatctcagt gcaacccagc accacggcgt cgcagccctg
     5101 gtgactcatt ctgccgatga cctgccgaaa gtaggcgcgc gattccgctg taaaacttcc
     5161 gttcaccagc tcatccatga tgatctgatc gattttcgcg cgctcgtccc catcgggcac
     5221 caggtattcg aagcccgccg cgtgcagctt gctctgatac accgtgcctt ccatggtgta
     5281 cttcgttccc gtaaccgcca gtttgtgata cccgtgtgac ttcgccacga cggcgacttc
     5341 gtcgacgatg tgcaaccaag gcagtggcga gcgcggggcg accagatcga acgcatgatg
     5401 gatagtgttg tcgggcgaga tgaggaagtc cgccccggcc gcggccagtt tgttggccga
     5461 agagagcatc agatcggcga ctccctgcca atcgaagcgg cggatcggcc ccatatactc
     5521 gcccagacaa tgcgtgtgca gggagatttc cggatgcata tggcgtccca tgagggcgga
     5581 agcttcgtcg cagatggtgc ggtagcacag agcggcaccc tcggcgctgc aggctacgat
     5641 tccaatgtgc ttgggcaagg ttctctcccg ggtcgaaaca aaaggcaagt ttgccacgga
     5701 tctgcactga tttcacggat agaacaaaat cgaaataggc cggagtcttg tccgtgtcat
     5761 ccgtttaaat ccgccgctgc ctgtgttcct agaacttcgc cagatactct cgctttttct
     5821 cctctggcag aaatgatgcg ttgaatgaat tctctgccaa ctgccgcaac tcggcctcat
     5881 tgaatccgaa cacttcctgc gcgatctggt attcgcgcgc cagactggtg cggaacatgt
     5941 cgggatcgtc ggtattcagc gtcaccagta tcccggcgtc gaacagcctg cgaatcgggt
     6001 gctgctcaac tgcggcacag catcccgtca gggcattgct ggtgatgcag gcttccaccg
     6061 ggacttgatg ttccgccaga aaacgcacga gatcaggatc gtcagtggca tgtaacccgt
     6121 gcccaatgcg gtcagccttc agctcgcgca gcgctgccca aatggactcc ggaccggcgg
     6181 tctctccggc gtgcacggtc aatcgcaatc catgagttgc agcgcgggca taaacatcgc
     6241 gaaacagctc aggcgctgcg cggcgttcat cgccgccaag gccgatgcca acgatggcat
     6301 cgctggtttt ccgcagcgca atggcttcgt cgagtacgag accggcccgc cgcgggccga
     6361 agtgccgcac ggcgtcgaag atccagtaga gcgacatccc aaaatcgcgc tcaccgcgtt
     6421 tgcgaccgcg ctccagacct tcaaacagcg gagcaaagtc gccgccgcgc caacggatga
     6481 cgccaaccga gacgtacact tccgcatgta gcacgttttg cgcgtgcagc ttctgcatca
     6541 tgcgataggt gacgagctcg tagtcgtctg gggtccgtag ccgctcggtg acagccttga
     6601 aggccaacag aaatccagtg aagttcttgt actggtagag cgcgtgggct tcctcttcgg
     6661 tgaagacgcg tccgctgtcg gcgatgttgg tgtagcgatt gttttcggtt gatagcggag
     6721 tgggatgccg tcggctcagt tccgccagcg tcgcgggatc gaccgtgccc tcgagatgca
     6781 ggtgcagctc cgccttagga agcgagacga tgaaggcgct gggagcgtcg ttgggcacgg
     6841 ctcggtggat tacctcagcg gctaaagccg cgcttgaaag tgtatggcaa gtgcaccggt
     6901 gaagcggtgc tccacccgta tggacacaag caaaattgga tcacctagcg tctccggtcc
     6961 tccgggcaga actcgctgcg atagcggctg tagagcatgt acaccgtgag tccgattgcc
     7021 agccacacga agaagcgaat ccaggtcagg atcggcaggc ccgacatcag cagcaggcag
     7081 aacagcacgc tgagaatcgg ggctgctatt ccacccggcg cgcgaaaccc acggcggcgt
     7141 tcgggctgtt tgtagcgaag gatgatgaca ccaatcgaga ccagcgcgaa agcaaaaagc
     7201 gtgccaatgt tcgagaggtc ggcaagtgtg ccgatgtcga gaatgccgcc gggaattccg
     7261 accaggaatc ccgcgatcca agtggcagtc gaaggagtgc ggaagcgggg atgcacgcgg
     7321 ccgaacaatt tcggcagcag gccgtcgcgc gacatggcga accatacccg cgcttgtccc
     7381 aactggaaca ccagcagcga cgaaatcatg cccatcatcg cgccgaacag gacagccagc
     7441 cttatccagt gcaggctatg cgaccctgtg gtaatgccga gttgcttgag gctgttgacc
     7501 acgggagcgg cgtcgtcgat caatcgctgc cagggcacga ttccggtgag caccacggcc
     7561 accgagatat acagcacggt gcagacgatg agcgtcgcga tgatgccgat gggcaaatcg
     7621 cgttgcggat ttttcgcctc ctcggcggcg gtcgagacgg aatcgaagcc aatgtaggta
     7681 aagaaaataa tggagccgcc ggtgagaatg cccgaccatc cgttgggcgc gaagggatgg
     7741 tagttcgcgg gatgaatgta atgcattccg gcgaacacga acgccaggat ggccgcgatt
     7801 ttcaggatga ccatgacgtt attggtctcg gctgattcgc ggatgccgcg caccagcacc
     7861 accgtgagca gcatgacaat gaggaatgcg ggaaagttga atccgaaatg ccatcccggc
     7921 gcatagagca tgtcgccttt caggtcggtc attcccgaag gcaggtaggc gggcgaaatc
     7981 cagcgcagcg caggatgcca gccgaaccag tcgaacatgt caaccaggtg cgccgcgaat
     8041 cccacgctca cggccatgtt gctgaccgcg tattccagga tcaggtccca gccgatgatc
     8101 caggcgatca gctcgcccat ggtggcatag gtgtaggtgt aggcgctgcc ggcgatggga
     8161 atcatcgacg ccaactcggc atagcacagc gccgtgaacg cgcagacgat cgccaccagc
     8221 accagcgaga cggccaacgc aggcccggct ccagggcggc cgagcgtcgc catgtgatgc
     8281 acgatgaagt cggcgagagg agcgttcaag atggacgagg tatcgaactt ctggcccgcg
     8341 atggcggttc cgatcacggt gaagatgccg gaaccaatga cagcgccaat gcccaaggcg
     8401 gtcagcgacc acgggcccag ggtcttcttc agccgatgtt ccggctcctc cgattcagag
     8461 atcagcttgt cgatcgactt acacgcgaag agttgtgtcg ccagaacagt gctcccgggg
     8521 atgctggaat cgatgcctca ggattcaaac agaaacccaa ggcaccgggc aagcgcaatg
     8581 tgtcgcagcg cttattggtt cttgtacacc actccgccct tcatcacgaa cttcacccgt
     8641 tccagcgtgg tgacatcctg caatgggtca ccgtccaccg cgatgatgtc agcgaaatga
     8701 ttgggctcga tcactccgac tttttcagac cagccgagca ggtcggcgtc attcactgtc
     8761 gccgcctgga tcgactgcaa cggcgtcagc cccagcttta ccatcacagc gaattcgtga
     8821 gcgttaaggc catggggata cacggccgcg tccgtaccga agccaacctt caccccgctg
     8881 gcgaaggcat gggcgacgtt tttgcgcgcg gccggcaaca ccgcttgggc cttagccagc
     8941 atctgcggcg ggacacccgt cttgggggcg ttttccaaga accagtcgcc gagatagagc
     9001 gtcggaacca ggtaagtgcc gtgctgcttc atggccgcga tggcagcgtc gtcgatgtag
     9061 gatccatgct cgatggagtc gatgccggct tctgcggccc agcggatgcc ttccgctccg
     9121 tgggcgtgcg ccgcgacttt tcggccaagt cggtgcgcat cggccacaat ggcggctaat
     9181 tccgcctgcg tatactgcga atgctgcgga tcgtcgccca gcgaaagcac gccgcccgtc
     9241 gcgcagactt ttatgacgtc agccccgtac ttgatgacct cgcgcacttt gtgctgcacg
     9301 ttttcgacgc cgtcggcaac gccgatctca gtcgcgtgga actcgtacgg aagcaggttg
     9361 ttatcgcagt gtccgccggt aataccgagc gctggaccgc tggcatcgat gcgggggcag
     9421 ggaacatcgc cggcattaat ggcatcgcgc agggccacgt cagtgaaagc attcgctccc
     9481 acgtttcgta ccgacgtaaa gccggccagc agagtgacgc gcgcgttttt ggcgccaatg
     9541 agggcctcgc gcggaaccga gatggcgaga gtgtcgtagc cgaacctggg attgaatgtg
     9601 aggtgggtat gcgcgtcgat caagccgggg agaacggttt tgcccgacag gtcgatgacc
     9661 gtggtgccgg cgtctgcggc tgcgcccaag ta
//
LOCUS       DASRKK010000039         9883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1370203, whole genome shotgun sequence.
ACCESSION   DASRKK010000039 DASRKK010000000
VERSION     DASRKK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370203"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VFC15_00815"
     CDS             <1..1615
                     /locus_tag="VFC15_00815"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM08733.1"
                     /translation="DIVAAMDRAEAGMIPERTRHLAFGDIAAVWSLLTDLDVAAVIDE
                     VVGARRSDAGASIGTYLALAALGRLVAPRSKKAFADWWATTAGDRFTKISPQVLDHRK
                     FWDAMHTVDAEALQDISARLAARMITMFDLDTSSVALDMTNFATYIDTTNNKAPIAQR
                     GKAKQKRTDLRLVGLGMVVTRAGGIPLVWHAYPGNRPDVTQFPTLIEHLKQRYTNLAD
                     HATDTDGAAVTDWPSMTVVFDAGQNSTANFEQLRRSGLHYVGSVPPSDCPDLLALPTA
                     DRKPVDKTRFPGVTAIDTRRVVYGQDRRVVLTHSQNLHDDQARSFTDTTLAKVGQKLD
                     DLAATLARGKTRRTREQLTTEIETITDSPWVHAVIDVELTGDSPADRRLTWTVNAVKR
                     QALEDNIFGKRILITDHDDWTVADVVAGYRSQSEIEFGFRQLKNPHEVSFCPMYHWTE
                     NNIAVHTFTCVLALQLAHLLRLQAHRAGHDLSVKALLDTLGGIQETVLIYPSTGGRPK
                     ARRMTTDMTVEQQALYDLFNLRRWAPTTT"
     gene            2362..3105
                     /locus_tag="VFC15_00820"
     CDS             2362..3105
                     /locus_tag="VFC15_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08734.1"
                     /translation="MAQLPDLERILDCAKLHRMPVELVTSAYWVDSLEIAEAVLDRLR
                     TRVHLLTITTRRSDVDSYGLAAIDLLLQAIRRFDISIQILVTVGPEEPFPKELLALEV
                     VNSDSTVIRIEPMYPNDRSQLSPQSGAWPEGFLMESPPRHARCAELLGFVITPGGDVY
                     PCSNGIGFPELLLGNMTTHTIVEMLDKALAGPQLNKLREEGPYFLFDDFRRSLPLEVL
                     PAGYVSSCDFHRQVMARNQQSASRVDSTA"
     gene            3125..4144
                     /locus_tag="VFC15_00825"
     CDS             3125..4144
                     /locus_tag="VFC15_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HZM08735.1"
                     /translation="MSQVVFNSLVLETVDRCNARCAMCYQAAAPKGSEIRGDDHLPLD
                     LVNRIIDEAAELPELMGDRVHISGGESFLFFDETVQIFKRAKARHFGNIGGTTNAFWA
                     LSDAIADRRCEELAQAGVTYLEVSIDHWHKPYVTADRVRCLLRAMRRTGLPVTLRTLS
                     SRSHHIDDVLEDFEDQELLQVIVGNGRVQPVGRGARTIPMSEVYIGGGVQGCCERQLN
                     LTISPNGNVYPCCAGADMTESLACGNIYRDSLAEAVLKMRTEAVIREVIHGGTGKLIP
                     IIEQLGYGDRLLPQYTSICHLCWDVFRQDDVAGALRQHYQDKQFEALVSALSSSVDEP
                     PLDAP"
     gene            4260..5309
                     /locus_tag="VFC15_00830"
     CDS             4260..5309
                     /locus_tag="VFC15_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZM08736.1"
                     /translation="MTNAENVLPVPWTALTSVLPSELISICAAKSEPILVEQFPDHPR
                     VFRIAFSEGDQDGLTSSSIVLKLATEWESWAQVAVHSRVADVVPRVFGTLPHESNFPL
                     GTVAILMEALTPSIPQVGVSVNSRQPIPHPKASYMRAIQKLAAVHHAFEGVDDSALRR
                     VSFEGLVNTVSAIPDLLTFLIAAGLELESALVDEVRTIGTHVYEHIYRCSGTPARTLV
                     HLDFHPFNVLLSASGAPRIIDWGSAAWGACELDLAMCDAEQVRIYFDARASLSNTDPN
                     GGYARLRSAVIVRMYEFIAAASGVLFGAAPVVRGDVMLNSIPLYVERLVSAASANEFM
                     GGEPIADMLRKTVRI"
     gene            5310..6218
                     /locus_tag="VFC15_00835"
     CDS             5310..6218
                     /locus_tag="VFC15_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM08737.1"
                     /translation="MKLRIERSGYHFFDRISGLHILFDEERPDADCVSMAPRSLSIAL
                     SNACDLKCHFCYREKNGAKLDERSIMQMAAVADELGCLEVTFGGGEPLLYRGLTTLCS
                     WIWSNTALGISITTHGHRLTEALISGLEGKISSLRFSIDGGEPYYGRIRGRPLKDLLL
                     RIEAVVGRIPFGINAVVSPGHTAELRCVAELAVQIGAMNLLIIPQHHQGQILLSEDEW
                     RELNDVVRDYESRLPLLVTASAASSILTGLLSTEREDEFLYAHISADGKLKANSYTAD
                     GLAIDDVSRLGTRFEQLQQSLYKGVV"
     gene            7260..7874
                     /locus_tag="VFC15_00840"
     CDS             7260..7874
                     /locus_tag="VFC15_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08738.1"
                     /translation="MTMHPKTAGAGTFLVRVFHRSALVAAVSGAILLVTGCGPDAATP
                     VTTTQPATTVPASTSIPTENQVATSLAAAAAAQSLAAQQSAASASASAALSLAAQQSA
                     ASASAAAQAQAEQEAAAASAAAQAQAQAEQEAAAAAEAARQSAAEQAQQAPDPSTAAP
                     QNSATVHAGAFCSPGGAIGVTVDGTPMVCKTTATDSRNRWRSAG"
     gene            complement(8025..8699)
                     /locus_tag="VFC15_00845"
     CDS             complement(8025..8699)
                     /locus_tag="VFC15_00845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM08739.1"
                     /translation="MTIRAVWFDVGETLIDETREYGTWADWLGVPRHTFSAMFGATIS
                     RGQDYREVFQNFKPGFDLETERSARLVAGRGEYLNAEDLYFDARPCLQQLKATGYFVG
                     IAGNQTKRAGRFIRELNLPADVIATSDDWGVSKPLPAFFQAVIRASGHRPEEIAYVGD
                     RLDNDIAPAMRAGLFAIWIRRGPWGFVLPPDTESPLLDDIEPNLTIDSLADLPGVLKQ
                     LTSNSP"
     gene            complement(8710..9372)
                     /locus_tag="VFC15_00850"
     CDS             complement(8710..9372)
                     /locus_tag="VFC15_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08740.1"
                     /translation="MAARGYYSVALESAREAGDHLQAASALGHTAFIPAAERGYQAAL
                     DYVAGANMHVSKRPYRLMSSWLAAVESELHTNAGSISAAQGAIARARSELAANELIGH
                     LAWFDYYDETRLDGFAGYVELRGGNFLEAQRTLTGALERLPLHAIKQRAIFLADLATV
                     HLNSGDVDQACCVAGMAADQLRRAGYATGSDRLREFRAAVDPWASSVAVRALDEQLAL
                     IA"
     gene            9690..>9883
                     /locus_tag="VFC15_00855"
     CDS             9690..>9883
                     /locus_tag="VFC15_00855"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM08741.1"
                     /translation="MPCIAQRNRPLLLQGTLTTFRRRCGKPTCRCAAGEPHESPALRF
                     TEDGRTKTLTLTAAEVAQVTA"
BASE COUNT         2101 a         2906 c         2946 g         1930 t
ORIGIN      
        1 ggacatcgtc gccgcgatgg accgggcgga ggccgggatg atcccggaac ggacccgaca
       61 cctggcgttc ggtgacatcg ccgcggtgtg gtcgttgctg accgacctgg acgtcgccgc
      121 cgtgatcgac gaggtggtcg gtgcccgccg ctcggacgcc ggcgcgtcga tcggcacgta
      181 tctggcgttg gccgccctgg gcaggctggt cgcgccccgg tcgaagaagg cgttcgccga
      241 ctggtgggcc accaccgcgg gggaccggtt caccaagatc agcccgcagg tccttgacca
      301 ccgcaagttc tgggacgcca tgcacaccgt ggatgccgaa gcgttgcagg acatctcggc
      361 cagactggcg gccaggatga tcacaatgtt cgacctggac acctccagtg tcgcgttgga
      421 catgacgaac ttcgcgacct acatcgacac caccaacaac aaggccccga tcgcgcagcg
      481 cgggaaggcg aaacagaagc gcaccgatct gcgcctggtc gggttgggga tggtcgtcac
      541 ccgcgccggg gggatcccgc tggtctggca cgcctacccc gggaaccggc ccgacgtcac
      601 ccagttcccg accctgatcg agcacctcaa acagcgatac acgaacctcg ccgaccacgc
      661 caccgacacc gacggtgcgg cggtcaccga ctggccgtcg atgaccgtgg tgttcgacgc
      721 cgggcagaac tccaccgcga acttcgaaca gctccgccgg tccgggctgc attacgtcgg
      781 gtccgtccca cccagcgact gccccgacct gctcgccctg cccacggccg accgtaaacc
      841 cgtcgacaaa acccggttcc ccggcgtcac cgcgatcgac acccggcgcg tcgtctacgg
      901 ccaggaccgg cgggtcgtgc tgacccactc gcagaacctg cacgacgacc aggcccgatc
      961 cttcaccgac accaccctgg ccaaggtcgg ccagaaactc gacgacctcg ccgccacgtt
     1021 ggcccgcggg aaaacccgcc gcacccgaga acagctgacc accgagatcg agacaatcac
     1081 cgacagtccc tgggtgcacg ccgtcatcga tgtcgaactc accggcgaca gccccgcaga
     1141 ccgccggttg acctggacgg tcaacgccgt gaaacgacaa gcgttggaag ataacatctt
     1201 cgggaaacgg atcctgatca ccgaccacga cgactggacc gtcgccgacg tcgttgcagg
     1261 ttaccgctcc caatcagaaa tcgaattcgg gttccggcaa ctcaaaaacc ctcacgaagt
     1321 gtcgttctgc ccgatgtacc actggaccga gaacaacatc gccgtgcaca cgttcacctg
     1381 cgtgctggcc ctgcaactgg cccacctgct gcgcctgcaa gcccaccgcg ccggccacga
     1441 cctgtccgtc aaagcgctcc tggacaccct cggcgggatt caggaaaccg tgttgatcta
     1501 cccctccacc ggcggacgac ccaaagcccg ccgcatgacc accgacatga cagtcgaaca
     1561 gcaagccctc tacgacctgt tcaacctccg cagatgggca ccgaccacga cttaggtcat
     1621 aggaaccaac actccctgca ccacccgtga cctgcaagaa cacccctcat gatcaactta
     1681 tctaggaaac tcccgctaga gcggacaacg ctcttcacat tcagaccttc ctgcatggag
     1741 caacgacctg gtcctaccct ggacatcccc gatttggact ttacggatac ggcggacaag
     1801 agtaacgttt cggtccaaca gcgagcacgc agccagcgag ccgctttcag agggggttgc
     1861 catgcctgca ccttcgaccg gcctgaccct tgacgaggtc aacttcttgc gaaggttgac
     1921 cagagagcca gactttcgtg cggaattctt ctccgatcga gaacgcgtag caaaggacga
     1981 aggccttgaa cttcgcacca gcaccttggt caatcgcgta acagcgaccg agatcgacgg
     2041 attgtatcgt gcagcgaacg cggttggcag agccgccgca gatgacaact gcacccttgt
     2101 atacgccttg gcgttcgccg tggcgttcgc cctactttaa cctcggccgg tatagctgtg
     2161 ccgctcaccg gaatccgacc ggccttcttc ggcgcggtcg ggttatatct aagcactgcg
     2221 tcacagcgca attggacaat gaccgctgat gaagcccttg gctatgttga agcaattggc
     2281 caacactctg atgcacaccg agggtcagtc gacgctgggc ttccgacccc cccaggcttg
     2341 tacgtcgccg gggccgagcc gatggcacaa ttgccggacc tggaacgtat attagattgc
     2401 gccaagctcc accgaatgcc ggtagagcta gtcacttccg cctattgggt cgactcatta
     2461 gaaatagcgg aagccgtgct cgacagactt cgcaccaggg ttcatctact gactataaca
     2521 actcgccgaa gcgatgtaga cagttatggt ctcgctgcca tcgacctgct tttgcaagcg
     2581 attcgacgct ttgacatcag catacagata ctcgtgacgg tcggtcctga ggagcctttt
     2641 cccaaagagt tgcttgcact cgaggtggtc aattcggact caacagtaat tcgaattgag
     2701 ccgatgtacc cgaatgaccg atcccagctg agcccacaat ctggcgcttg gcccgaagga
     2761 ttcctaatgg agtctcctcc ccgccacgcc agatgcgcag aactactggg cttcgtgatc
     2821 acgccaggcg gcgacgtcta tccatgctcc aacggaattg gctttcctga actgctcctc
     2881 ggtaatatga ccacacacac gattgtagag atgctcgaca aggctctcgc cggacctcaa
     2941 ttaaataagc ttcgtgagga agggccctac ttcctgttcg atgacttcag acgctcactg
     3001 ccactagaag tgctaccggc aggctacgtc agctcatgtg actttcatag gcaagttatg
     3061 gctcgaaatc agcagtccgc atcacgtgta gattccaccg cctgacgaat ggggcaaaag
     3121 cgttatgtcg caagtggtat tcaactcgct ggtgttagag accgttgacc gttgcaacgc
     3181 gcgctgcgcg atgtgctacc aagccgcggc gccaaaggga tcggagattc gcggtgacga
     3241 ccacttgcct ctggatctcg ttaatcgaat aatagacgag gcggcggaac tgcccgaact
     3301 catgggcgac agggttcata tctcgggggg tgaaagtttt ctgttcttcg atgaaactgt
     3361 tcaaatcttt aagcgcgcaa aggctcggca ttttggcaac attggaggaa ctacgaacgc
     3421 attctgggcg ctctccgatg ccattgcgga ccgaaggtgt gaagaactgg cccaagccgg
     3481 cgtgacctac ctcgaagtgt cgatcgacca ctggcacaag ccgtatgtta ccgcggatag
     3541 ggtgaggtgc ctcttgcgtg cgatgcgccg aactggcctc ccagtgactt tacgcacttt
     3601 gtcgagccgc agccatcaca ttgacgacgt gttagaagac ttcgaggatc aggagctact
     3661 ccaagttatt gtaggaaatg gtcgagtgca acccgtcggt agaggagcca gaacgatccc
     3721 aatgtcggag gtgtacatag gtggtggcgt gcaagggtgt tgcgagcggc aactaaactt
     3781 gacgatttca ccgaatggaa atgtgtaccc gtgttgtgcg ggcgctgata tgaccgaatc
     3841 gctagcatgt ggcaacattt acagggactc gcttgccgag gcggttctca agatgaggac
     3901 cgaggcggtg attcgtgagg tcattcatgg cggtaccggc aaacttatcc cgatcattga
     3961 gcagctgggc tacggtgatc gactgttgcc acaatatacg agcatctgtc acctgtgctg
     4021 ggatgtattt cgccaagacg atgtagcggg tgccttgcgg cagcactacc aggataagca
     4081 gttcgaagca ttggtttccg cgctatcaag ttcggtcgat gagccgccat tggatgctcc
     4141 atagtgtacg tgcatttaga gggcgtacga ccacgtaagg cgccaggcat ccgattatgc
     4201 gcaagttcag gtcaagatct tggttcccaa gcctcgtgca gagagggcca cgcttgatcg
     4261 tgactaatgc cgagaacgtc ctcccagttc catggacggc gttgacctcc gtcctaccta
     4321 gcgagctaat atcgatttgt gcggctaaga gcgagccgat tcttgtcgag caatttccgg
     4381 atcacccgcg agtatttcgg attgctttta gcgaaggaga tcaagacgga ctcacgtctt
     4441 catcaatagt cctgaaatta gcgaccgaat gggagagctg ggcacaggtt gctgtccata
     4501 gccgtgtagc cgatgtcgtc ccgagggttt ttggcacatt gccgcatgaa tctaacttcc
     4561 ccttaggcac ggtggccatc ctgatggaag cgctgacgcc gagcattcca caagtcggcg
     4621 tcagcgtaaa cagtcggcaa ccgatcccac acccaaaggc cagctatatg agggctattc
     4681 agaagctggc ggcggtgcat cacgccttcg aaggcgtcga cgacagcgct ctgcgcagag
     4741 tctcattcga aggtcttgtg aacaccgtgt ctgcgatccc ggatctctta acatttctca
     4801 ttgccgcggg gctggaactc gaatcggccc ttgtagacga agttcgcaca atcggcacgc
     4861 atgtttacga gcacatatac cgttgcagcg gcacaccagc tcgaacgctg gtccatctag
     4921 atttccaccc cttcaatgtt ctcctctccg cgagtggagc gcctcggata atcgattggg
     4981 gaagcgcggc gtggggggct tgtgaacttg atttggcaat gtgcgacgca gagcaggttc
     5041 gtatatactt tgacgcacga gcaagtctat caaataccga tccgaatggg ggatatgcgc
     5101 gtttacggtc ggcagtcatt gttcgaatgt acgaattcat cgccgccgcg tcgggtgtgc
     5161 tattcggggc ggcgcctgta gtgcgtggag atgtcatgtt aaatagcatc ccactgtatg
     5221 tggagcggct cgtgtccgcg gcgagcgcca atgagttcat gggcggcgag ccaattgctg
     5281 acatgcttcg aaagactgtg aggatttaat tgaaactgcg catcgagagg agcggctacc
     5341 actttttcga ccggatatcc ggcctgcaca ttctgttcga cgaggaacga ccggacgctg
     5401 actgcgtcag tatggctccg cgaagtctca gcatcgcgct atcaaacgca tgcgacttga
     5461 aatgccactt ctgttaccgg gagaaaaatg gagcaaaact agacgagcgg tcgattatgc
     5521 aaatggctgc ggtggccgat gaacttggtt gcctcgaggt gaccttcgga ggtggggagc
     5581 cgcttttgta ccggggtctg acgactctat gtagttggat atggagtaac accgccttag
     5641 gtataagcat taccactcac ggacataggt tgacggaggc attgatctca gggctcgagg
     5701 ggaagatttc gtccttgaga ttttccatcg acgggggtga gccgtactat ggcagaatcc
     5761 gtgggcggcc ccttaaagac ttactcctaa gaatcgaggc cgtagttggg cgcataccct
     5821 tcggcatcaa tgcagtagtt agtccaggtc acacggctga gcttcgctgt gtagccgaac
     5881 tggccgttca gattggagcg atgaatttac tgatcatacc ccagcaccac caaggtcaga
     5941 ttttgctcag cgaggatgaa tggcgtgagc tgaacgatgt tgtgcgcgat tacgaatccc
     6001 gattgccgct gctggtaacg gcgagcgccg cctcctcaat cttgacgggc ctgctatcga
     6061 ccgagcgtga agacgaattt ttgtacgcgc atattagcgc agatggtaaa ctgaaggcaa
     6121 attcatatac tgcagatggc cttgcgattg atgatgtatc gcgcctggga acacgcttcg
     6181 aacaattgca gcagagcctt tataagggtg ttgtatgaag gtttggcgaa cgcatgcagc
     6241 tgaacattcg gcaagcgtca agattgtggg ccggttcgca tcgccggcgg atgctcaagc
     6301 cgctgtcaga atgttcaacg atctactttc catcggtact cggctggaaa gtgggtcggc
     6361 gggggcggtg gaggagcttg aaagctttcg cacggcgagc ggactccagc tgttgagtga
     6421 gtccgatttg gtagagctgc ggcaattcga gcccattgcg gccggcggga gggagattag
     6481 ggttgagaca gaagcccagc acgccgatgc actgctgaaa atcgtcgcgt tatttgatgc
     6541 gcaagtgcaa gttcacaggc gggagggtga ttggccccct cctggggccc atgggtcgtc
     6601 cgccgggtcg ggcgcgtgaa ggggttcggt cggatcggat gcgccggtca gcgtcacgcc
     6661 ccggtttcgc ggacgcggca tggccaaggt atggaccgtc ccttccaagc cgccgctgac
     6721 caacccactg tccgtcggca gggtcagtga ccgcccaaac cgggtgaccc gggtgttcgg
     6781 caaaacgggc ccgacagggg cttgcacctg acaagctctc cgattgggcc acttttccgc
     6841 atcgcggcca ctggcgcagt cttgaatgag tagagaaagt cctcacgagc gttcggctcg
     6901 gccaatgtcc ggaccgtcgg atctgtgagt tggtcacagg gttcgagcgg cctccgacgg
     6961 acggcgagtg gttcggcatt cggtggtgca gtagaccgtg agggaacgca catagacgcc
     7021 tcgtccagcc agcgcaatgc ggtggaggcc acgcgggttg aggctgcttc cgcaggtcac
     7081 agcccttcat gacccgccga gcagcccacc caacccggaa gcgactacag ccgaactgcg
     7141 ctttcggagg tgtgacgcgc ctggtgcgac aacgcccgaa tggcgccaac tggttacaga
     7201 ccggacactg tacgtatatc tgtcggcagg ggggtccagc cgacaccaaa gggcatacga
     7261 tgacaatgca tccaaagacc gctggcgccg ggacctttct tgtgcgcgta tttcaccgat
     7321 ccgcgctggt tgctgcggtt agcggggcaa tactgctggt cactggctgc ggtccagatg
     7381 ccgcgacccc cgtaacgacc acgcagcctg ccacgacggt tcccgcgtcg acctcaattc
     7441 caaccgagaa ccaggtcgcc acgtcgctgg cagcagcggc agccgcccaa tccttagcgg
     7501 cgcagcaatc ggcagcgtca gcttcagcgt cagcagccct gtccttagcg gcgcagcaat
     7561 cggcagcgtc agcctcggca gcggcccaag cccaggcgga acaagaagca gcggcagcct
     7621 cggcagcggc ccaagcacaa gcacaagcgg aacaagaggc agcagcggcg gctgaggcag
     7681 cgcgtcagtc agccgccgag caggcccaac aggcaccaga tccttccact gctgcaccac
     7741 aaaactcagc aaccgttcac gccggagcgt tttgctctcc aggcggcgcg atcggcgtga
     7801 cagtcgatgg caccccgatg gtctgcaaaa cgacagcgac cgactccagg aaccgttggc
     7861 gcagtgccgg ctagctgaca gaactacatc gctggggttc cgagtagggc gtgtgaaagc
     7921 ccccgtgttc ggtcgcggtg cggttgaggc ggaggcgaca tgtgggggac ggtgatccct
     7981 ggcgccagtt gccccactgt ggtagtccag ttgaaggaat tacttcaagg cgaatttgat
     8041 gtcagttgtt tgagcacccc agggagatca gcgaggctat cgatcgtaag gttcggctct
     8101 atatcgtcga gcaatggtga ctccgtgtcc gggggcagaa cgaagcccca cggcccgcgg
     8161 cggatccaaa tggcaaaaag gccagcccgc atggcgggcg cgatgtcgtt gtcgagccga
     8221 tcaccgacat aagcgatctc ctcaggtcgg tgtccggacg ccctgatgac ggcctggaag
     8281 aaggcgggca acggcttact cacgccccaa tcatcggaag tcgcgataac atccgctggc
     8341 aggtttagct cacgtatgaa gcggccagcg cgtttcgttt ggttgcctgc gattccaacg
     8401 aaatagccag tagccttcaa ctgctgcaag catgggcgag cgtcaaaata gagatcctcg
     8461 gcgttgagat actcaccccg tccggccacg agccgagcgc tccgctccgt ttcaagatcg
     8521 aacccaggtt tgaagttctg gaagacttcg cggtagtcct gtccgcgaga gattgtagcc
     8581 ccgaacatcg cggagaacgt gtgacgagga acgcccaacc agtcggccca agtcccgtac
     8641 tcgcgggttt cgtcgatgag cgtttcgcct acgtcgaacc agacggcgcg gattgtcata
     8701 cgtatatcct caggcgatca gggcgagttg ctcgtcgagt gcgcggaccg caaccgatga
     8761 ggcccaggga tcgactgctg ccctaaattc tcgaagtcga tccgaaccgg tggcgtagcc
     8821 ggctcgacga agttgatcgg cggccatacc tgcaacgcaa catgcctggt cgacgtctcc
     8881 gctattcaag tggacggtcg caaggtctgc caggaatatt gcgcgctgct tgatggcgtg
     8941 caaaggaagg cgctcgagtg caccggttag cgtgcgttgc gcttccaaga agtttccgcc
     9001 gcgcagttcg acgtaaccag cgaatccgtc gaggcgagtc tcgtcatagt agtcgaacca
     9061 ggcaagatgc cctattaatt cgttcgcggc gagttccgag cgagcgcggg ctatggcccc
     9121 ttgtgcggct gagattgacc ctgcgttggt atgtaattcg gactccacag cggcgagcca
     9181 tgaggacatg agccggtagg gtcgtttgct gacgtgcata tttgcgccgg cgacgtagtc
     9241 gagggcagct tgatagcctc tttctgcggc cgggataaag gcagtgtgac cgagcgccga
     9301 cgcggcttgg aggtggtcgc ccgcttcccg cgcggactcc agggcgacgc tgtagtagcc
     9361 acgtgccgcc atggtgtcat ggaggtcaaa gaatgacagc ctaccagcta atatcgctac
     9421 tctggccctg ttcgtgagaa gcttgcgatg cgtggatggc gtgacattgt cacccagcaa
     9481 ttcggcaacg gtttcgaggt gggcaataac cggtatcaat agagccgagg gggccgtcga
     9541 atggtaaggg tgcggctcgt ttggagtgag gcaccgacgc gggtgactac atcggcttga
     9601 ttgcctgaca cgcactggaa atccccgaag tacggctgtg tgcacggtta cctgtctgta
     9661 tccgcgaatg gatacagaca ggggttagca tgccatgcat cgctcaacga aaccgaccac
     9721 tgctgctgca aggcaccctg accacgttcc ggcgccgctg cggcaaaccg acctgccgct
     9781 gcgcggcggg agagccgcac gagagccccg cactgcggtt caccgaggac ggccggacca
     9841 agacgctgac gttgaccgcc gccgaggtcg cgcaggtcac cgc
//
LOCUS       DASRKK010000040         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_99807, whole genome shotgun sequence.
ACCESSION   DASRKK010000040 DASRKK010000000
VERSION     DASRKK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99807"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..556
                     /locus_tag="VFC15_00860"
     CDS             155..556
                     /locus_tag="VFC15_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZM08742.1"
                     /translation="MHRLTIKTHKKREIVDITEQLESFLWKQYGDKTGVCQLSVLHTT
                     AALTTADLDPGTDLDMLDAFEHLIPKLQYRHPHNPAHTPDHILSTLIGTSLALMLDRG
                     LLLLGTWQRVVLVELDGPRERELVLAFVETA"
     gene            complement(853..1827)
                     /locus_tag="VFC15_00865"
     CDS             complement(853..1827)
                     /locus_tag="VFC15_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZM08743.1"
                     /translation="MVRIEAEALRALADRLAGPMAADFERAVELLYCCAGRIVVTGMG
                     KSGLIARKIAATLTSTGSPALFLHPAEALHGDLGMVVRGDVVVALSASGETDEILQLL
                     ATVKRLGVPLITMTCDDLHSLPEGPRSMSTLAQAADIALDCSVAHEACSLGLAPTAST
                     TSMLALGDALAVALSQRRGFKEEDFANLHPGGKLGKRFLRVSALMRAGNAVPQVKPET
                     AMSDVIYEMSRKGLGMTTVVDGKKLVGLISDGDLRRLLEQHGKSVFELAAAECMTRSP
                     RTVGPDVFAIAALDVMEQKKITSLPVVNAAGHLLGVLHLHDLWGTQML"
     gene            complement(1884..2909)
                     /locus_tag="VFC15_00870"
     CDS             complement(1884..2909)
                     /locus_tag="VFC15_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZM08744.1"
                     /translation="MGAFGERLRREREMRNISLDEIAAVTKIGTRLLRALEDEQFDLL
                     PGGIFNKGYVRAYAKYVGIDEEQAVADYLQAAHEAAPDVHGIAEENTSSRFEGASREQ
                     LSAPRRSTFPVVPVLIVLVLIAGIAGGWRLYRERQFDRRASVATAVTRDTTLHDSTVS
                     PPATSMSTPGKSPSPQTGSGMTDHGGTDVISSAKPVDAGASAPGASPAASDGRTGQTA
                     ATQLVSGQNATKPLPPAAPMSLATGTSNVANSAGATSFELTVRPKDRAWVSVKSDGKF
                     VVRGIIKPPDVKTIRATSQVVFWTGNAGAVEVSFNGKSVPLTGGENEERVLVFNSRGL
                     LPPSASQ"
     gene            3185..4255
                     /locus_tag="VFC15_00875"
     CDS             3185..4255
                     /locus_tag="VFC15_00875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZM08745.1"
                     /translation="MSLPATDNDRQFYELKIFHDVAKALTSSLDLDTILQTIMEKMAA
                     YFQPATWSLLMLDEATDELYYAAAVGKGCENLNALQLASGESLARWVITHGTPLVIPD
                     VTQDERIRQASGDSFFVTGCSVVCMPVRTGGKILGIIQMVNIDMQVYTRNEMLLRTLA
                     DYAAIAIENARAVQRIQELSITDDCTGLYNARQLSIALSDEVHRSERFGYEFSLLFLD
                     LDYFKRVNDEHGHLVGSKLLGQVGQALRENLRLVDAAFRYGGDEFAILLPQTSKDAAL
                     LVARRLMALFHSRQWLAAEGVPLSLRASIGVASYPADAVSPQAIVQRSDEMMYQVKQA
                     GRDNIALAGVGIVGSEEHPPKF"
BASE COUNT          866 a         1301 c         1346 g          932 t
ORIGIN      
        1 aagttttgtt gccttggaac cgcagttcaa tctacccgat ccttacaaca agaattgggg
       61 gagcgtcgac accggcatgg tgctactgca gcctgggcaa tccgtgtcct ggcgggtgcg
      121 attagaattg ttcacgccga gcggtaggtg agtcatgcat cggcttacga tcaaaaccca
      181 caagaagcgc gagatcgtgg atatcaccga gcagctcgaa tcttttctgt ggaagcagta
      241 tggcgacaag accggagtat gccaactgag tgtgcttcac acaacggccg cgctgaccac
      301 ggccgatctt gaccccggaa ccgacctcga catgctcgac gccttcgagc acctcattcc
      361 caaacttcag tatcgccatc ctcacaaccc ggcgcacacg cccgatcaca tcctgtccac
      421 gctgatcgga acttcgctgg cgttgatgtt agatcgcggt ttgctgttgt taggaacctg
      481 gcagcgggtc gttctggttg aactcgacgg gccccgcgag cgcgaactgg tgctggcgtt
      541 cgtggaaaca gcttagagcc attccataat tggtgtatcc ggccgtgcgg gtagtgcggg
      601 cctttaggcc cgcgtaatag gggcaggaag aaaatcgcta aagccgctta ggtcttcgac
      661 ctcagcggct ttagcggacg tttggccgcg acgaacgcgg cgctgaagcg ccgcactacc
      721 cgtacaaacc ggatacatct attgcgaaac cgctccagca gtcagcactc agccaaacaa
      781 ggcagacggg cttcagaaga cccgcttccg catgcaaggc tgagtgctga gtgctgagtg
      841 ctgacggctt cttcacaaca tctgcgtgcc ccacagatca tgcaggtgca gcacgcccag
      901 caagtggccg gctgcgttca ccacgggaag agaagtgatc tttttctgct ccatgacgtc
      961 gagcgcggcg atggcgaaca catcagggcc gacggtgcgc ggcgaacgcg tcatacattc
     1021 cgcggccgca agctcgaaca cgctcttacc gtgctgttcc agcaagcggc gcaggtcgcc
     1081 atcgctgatc agtcccacga gcttcttgcc atcgaccacg gttgtcattc ccaagccctt
     1141 gcgcgacatt tcgtagatca cgtcgctcat ggctgtctcg ggtttgactt ggggcacggc
     1201 gtttcccgcg cgcataaggg cagagacgcg cagaaatcgt ttgccgagtt tgcctccggg
     1261 atgcagattg gcgaagtcct cttccttgaa tccacgccgt tgcgagagcg cgaccgcgag
     1321 cgcatctccc agtgcgagca tggaggtggt tgacgcagtg ggcgcaagtc cgagggagca
     1381 ggcttcgtgg gcgaccgagc agtccagcgc gatgtcggcg gcttgtgcca gcgtggacat
     1441 gctgcgcggc ccttcgggaa gggagtgaag gtcatcgcaa gtcatggtga tgagcgggac
     1501 gccgagccgc ttcacggttg ccagcagttg caggatttcg tcggtttcac cactggccga
     1561 gagcgcaacc acgacgtccc cgcggaccac cattccgagg tccccgtgca gggcttccgc
     1621 aggatgaaga aacaacgccg gacttccggt agaggtgagg gtcgcagcga ttttacgggc
     1681 aatcagcccg ctcttgccca tgccagtaac gacgatgcgg ccggcgcagc agtagagcaa
     1741 ctccaccgcc cgttcaaagt ccgctgccat cggccccgca agacggtcgg cgagggcgcg
     1801 cagggcttcg gcctcaatac gcaccacgtt ttcaccaagg ttcttgctgg tgggtcgggt
     1861 tgaaggttcg ctgccaggcg gcgtcattgg gaggccgaag gcggcagcag tccacgcgaa
     1921 ttgaagacga gcacgcgttc ctcattttcg ccgccggtga gaggaacgct cttgccatta
     1981 aacgaaactt ccaccgcgcc ggcgttgccg gtccaaaaga ccacctggct ggtggcgcgg
     2041 atggtcttca catccggcgg cttgatgatg ccacgaacca caaacttacc gtcggacttg
     2101 acggaaaccc aggcgcggtc cttggggcgg accgtgagtt cgaaggacgt agcgcctgcg
     2161 gaattcgcaa cgttacttgt tcctgttgca agactcatgg gagccgctgg tggcagcggc
     2221 ttggttgcgt tttggccgga aacgagctgg gttgcggcgg tctggccggt gcggccgtcg
     2281 ctggctgccg ggctcgctcc gggcgcgcta gctcccgcgt cgaccggctt tgcgctgctg
     2341 ataacgtctg ttcctccatg atcagtcatg ccagagccgg tctgaggcga aggcgatttt
     2401 cctggggtgg acatgcttgt tgccggaggg ctgacagtgg aatcgtgcag cgtcgtatcg
     2461 cgcgtcactg cggtcgccac gctcgcgcgc cgatcgaatt gacgctcccg gtaaaggcgc
     2521 cagcctcccg cgattcccgc aatgagtacg agaacgatga gtacgggaac cacaggaaat
     2581 gtcgatctcc gcggggcgct taattgctcg cgactggccc cctcgaatcg gcttgaggtg
     2641 ttctcctcgg cgatcccatg tacgtcgggc gcagcttcgt gcgcggcttg caaataatcg
     2701 gcgactgcct gctcctcgtc gattccgaca tatttggcgt aagcgcggac atatcccttg
     2761 ttgaagatgc cgccggggag cagatcgaat tgttcatctt ccaaggcgcg aagcaggcga
     2821 gtcccgatct ttgtaactgc ggcgatttcg tcgagtgaga tgttcctcat ctctcgctcc
     2881 cgacgcaacc gttcgccgaa tgcacccatc ccagcctcgg tctgctccaa cgacttacgc
     2941 aattgaattt accagtgccc gtgccgatca tagaaagcgg tgcaggcagt gtcaaactgg
     3001 ccgccaaccg ggagtgggtc agtttgccta gctgaaaact actggaatag gaaccacctg
     3061 cgctttccgg taaaatgcac ggtaggcagg cgtatattct tgagcctgga tcttttggaa
     3121 tcgaggggcg cttggctctg gttccctttt ccgggggcag acgagatctg cgctaggttc
     3181 ttgcatgtcg cttccagcta cggacaacga tcggcagttt tacgaactaa agatctttca
     3241 cgacgtcgcc aaagccctga catcgtcgct tgatctggac accattctgc aaaccatcat
     3301 ggagaagatg gctgcctatt ttcagccggc tacctggtcg ctgctcatgc tggatgaggc
     3361 cacggacgag ctctattatg cggccgcggt cgggaaaggt tgcgagaacc tcaacgcctt
     3421 gcaattggca tcgggtgaaa gtctggcgcg atgggtgatc acccatggca caccgctggt
     3481 tattcctgac gtaacccagg acgaacggat ccggcaggcg agcggtgaca gtttcttcgt
     3541 caccggatgt tccgtggtgt gcatgccggt gcggacgggc ggcaaaatcc tgggcatcat
     3601 ccagatggtg aatatcgata tgcaggtcta tacccgcaat gagatgctgt tgcggacctt
     3661 ggccgactac gccgccatcg ccatcgagaa cgcccgtgct gtccagcgca ttcaggaact
     3721 cagcatcacc gacgactgca ccggcttgta caacgcacgc cagttgtcta tagcgctctc
     3781 tgacgaagta caccgttcgg aacgtttcgg atatgagttc agccttctgt ttctcgatct
     3841 cgactacttt aagcgggtga acgatgagca cggacacctg gtcggcagca agcttctggg
     3901 gcaggtgggg caggcgttgc gggaaaacct tcgcctggtg gacgccgcct ttcgctatgg
     3961 cggcgacgaa ttcgccattc tattgccgca aacttcaaag gatgcagctc tcctggtggc
     4021 gcgccggctg atggcacttt ttcactcccg gcaatggctc gctgcggagg gtgtgccgct
     4081 atctctacgg gcgagcatcg gtgttgcaag ttatccggca gacgccgttt ccccgcaagc
     4141 catcgtgcaa cgctccgatg aaatgatgta tcaggtgaag caggcggggc gtgacaacat
     4201 tgccctagcg ggtgtcggga tcgtgggatc cgaggaacac ccccccaagt tctaatcccg
     4261 cagtgccaac tccccgctgt cgcgggtttc cttatttcgg ctgcacttcc gtagtggttt
     4321 tgtcgggttg caccgtattt ggatttgccg gagcgttggg attgacgccg aagtcccaga
     4381 cgtaaagatt catctcctgt gggccgagca tctccaccac ggcatactcg ccgggggcga
     4441 ggtcc
//
LOCUS       DASRKK010000041         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_881956, whole genome shotgun sequence.
ACCESSION   DASRKK010000041 DASRKK010000000
VERSION     DASRKK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881956"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..2032)
                     /locus_tag="VFC15_00880"
     CDS             complement(191..2032)
                     /locus_tag="VFC15_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM08746.1"
                     /translation="MKYVLRILLFLSLISTFSAAQSMNRMSHPNQANAKFAQMTDQFV
                     KDSLVLSPVSASQAGYHKHLDPKTGKTIDLDAQLDDVSAQGIAAQEKFYREWRHRFQT
                     ETPVAGLNAQDAADYRLIDDQIALSLLEFDQIQNYKHNPTGYVELIGNGLFLPLTQEY
                     ASKEVRVGDVISRIGQIPRFLDQTKSQLTDSDPIFISTAAGENEGNINLVDSVAADIP
                     AGSPLKAQYDKVAPAAKRALTDFNVWMQNDLAKRPANGRTWRLGKEWYTPKFRYVMET
                     SIEPAQLLADAEAALTKTRAEMLQLALPLYKQMYPGQDDYSNLPAQERENRIIAAVLN
                     KISDEHPQRDQLIEAVKADLDGIKQFIREKKIVSLSSRDNLKVIPTPEFERGSYSVAG
                     FHSAPPLEPTAEAQYWVTPIDPKMSDAMVESKLREYNNYALKWLTIHEALPGHYVQAE
                     HADDVEPPTRRVLRNLFGNGPYVEGWAEYGADVMTQEGYLVFSPKFRIVRLKIWLRGV
                     ANTILDIRLQTMNMTDQQALDLMMKDTFQTQAEAEGKLVRAKLNSTQLPTYFVGTRQW
                     WSLRKKYQAAKGSSFTLEEFHNRALDQGPLPIEYLEKIILPESGAGN"
     gene            2596..>3030
                     /locus_tag="VFC15_00885"
     CDS             2596..>3030
                     /locus_tag="VFC15_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZM08747.1"
                     /translation="MKRVLVATSNPGKIRDLVGAAAAHDVQIETLPGYSSLPDVIEDG
                     LTFEANARKKAEHYSKYADGELVIADDSGLEVDAIGGAPGVRSARYAADERNAPEHAP
                     NSDDEANNARLLRESHDVPDEYRTGRFVCVIAVARNGHTLATF"
BASE COUNT          590 a          882 c          886 g          672 t
ORIGIN      
        1 attgggatgt atgcagcgca gtaagtaaat ggacggcggg cgccgttttg tctttctgtt
       61 gggttgcggc gcccgccttg gatttgcgtt acctctatag acggatggcg gcgggaaaag
      121 ttctcctcac gctcgacaat atcgatctct taacacttct tcacactcgt cgttgtcgaa
      181 tgcgctactg ttaattccct gctccacttt ccggcaaaat gattttctcc agatactcga
      241 tcggcaatgg cccctggtcg agcgcgcgat tgtggaactc ctccagcgtg aagctgctgc
      301 cttttgcagc ctgatatttc ttgcgcaatg accaccactg gcgagtaccg acgaagtacg
      361 tcggaagctg ggtcgagttg agcttggcgc gcaccagctt gccttcggct tcggcctgtg
      421 tctggaaggt gtctttcatc atcaggtcga gcgcctgctg gtccgtcatg ttcatggtct
      481 gcaggcgaat gtcgaggatg gtgttggcta ccccgcgaag ccaaatcttc agccgtacaa
      541 tccggaactt cggactgaaa acgagatagc cttcctgcgt catcacgtcg gcgccgtact
      601 cggcccaccc ttcgacgtac ggtccattgc caaagagatt gcgcaacacg cgccgcgtcg
      661 gcggctcaac gtcgtcggca tgctcagcct gcacgtagtg tcccggcaag gcctcgtgaa
      721 tggtgagcca cttcagcgcg tagttgttgt actcgcgcag cttgctttcg accatcgcat
      781 ccgacatctt aggatcgatc ggagtcaccc agtactgagc ttcagcggtt ggctccagcg
      841 gcggcgccga gtggaacccc gccaccgaat agctgccgcg ctcaaactcc ggcgtcggga
      901 taactttcag attgtcgcgg ctgctaagcg agacaatctt cttctcgcgg atgaactgct
      961 tgatgccgtc gagatcggcc ttgaccgctt cgatcaactg atcgcgttgc ggatgttcgt
     1021 cgctgatctt gttgagcacc gcggcaatga tcctgttctc gcgctcctgg gcgggcagat
     1081 tgctgtagtc gtcctggccg ggatacattt gcttatagag cggtagtgcg agttgcagca
     1141 tttccgcgcg agttttggtc agcgcggcct cggcgtcggc cagcaactgc gctggctcga
     1201 tggaagtctc catcacgtag cgaaacttgg gcgtgtacca ttctttgccc agccgccagg
     1261 tgcggccgtt ggccggacgc ttagccagat cgttctgcat ccacacgttg aagtcggtca
     1321 gcgccctttt ggccgcaggc gccactttgt cgtactgcgc tttgagcggc gaacctgccg
     1381 ggatatcggc ggccacggag tccaccagat tgatattgcc ttcgttctct cccgccgcag
     1441 tcgagatgaa gatgggatcg gagtccgtga gttgcgactt ggtctgatca aggaaccgcg
     1501 ggatctggcc gatgcgcgag atcacgtctc ccactcgcac ctcctttgac gcgtactcct
     1561 gcgtcaaagg cagaaacagg ccgttgccga tcagttccac gtaccccgtg ggattgtgct
     1621 tgtaattctg gatctggtcg aattcgagca ggctcagtgc gatctggtcg tcgatcaggc
     1681 ggtagtcggc ggcgtcctga gcgttcagac ccgcaaccgg tgtctcggtc tggaagcgat
     1741 gtcgccactc gcgatagaac ttctcctgag cggcaattcc ctgcgcacta acgtcgtcga
     1801 gctgcgcgtc gaggtcaatt gtcttgccgg tcttggggtc gagatgcttg tggtaaccag
     1861 cctgcgacgc gctcacggga gacagcacca gcgaatcttt cacgaactgg tctgtcatct
     1921 gggcaaattt cgcattggct tgattcggat ggctcatgcg gttcatggat tgtgccgcgc
     1981 tgaatgtgga gatgagcgag agaaacaaaa gaatgcgcaa aacgtatttc atagcgcctc
     2041 ggaagaagaa gagtgcaagc gggctagtat aaacccgtaa gctattccgc ggcctcggtt
     2101 cggcgacgga tagatacccg gcaatcggcg gcttcacctt cgcgcgaaac tctttgctga
     2161 ggtaaagaaa gccgcagccg aagggcgccc tgagccactt gtgtcccccg caggcgattg
     2221 cgtccaccgg agttttccgc acatcgaacg gaatcgctcc gagttgctga actgcgtcga
     2281 aggtcccggc gggcagtacg ccgccatgcc gaggaaactg cgcagcttct cgaccgcgcg
     2341 ctgcggagcg aggctttagc cgctgaggta accggagccc ttacctcagc ggctaaagcc
     2401 tcgctcttca tatgcagtct tggtatgcac gcctgaaagg cgtgctccac ccactactgg
     2461 atacagaaac ttctggaacc cctttatcgt gtgggacaca actgcgttta ggacattgta
     2521 caaaagaaaa cggcaggggc gtgcatggct ccgcgttgtc tgcggattct gatacgttaa
     2581 acgcaggagg cgctgatgaa acgcgtactg gtcgcgacgt ccaaccctgg gaaaattcgc
     2641 gaccttgttg gagccgccgc ggcccatgac gtgcaaatcg aaacactacc cggctactca
     2701 tcgctgccgg atgtcatcga agatggcttg accttcgaag ccaatgcgcg caagaaggcc
     2761 gagcactaca gcaaatacgc cgatggcgag ttggtaatcg ccgacgactc gggcctggaa
     2821 gtcgatgcga ttggcggcgc tccaggggtt cgctccgcac gctatgcggc cgacgagcgc
     2881 aacgcaccag agcatgcgcc aaactccgat gacgaagcca ataacgcgcg cctcctgcgc
     2941 gaatcgcatg acgttccgga cgaataccgc actggcaggt tcgtctgcgt tatcgccgtg
     3001 gcccgcaacg gccacacctt ggccactttt
//
LOCUS       DASRKK010000042         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_2437, whole genome shotgun sequence.
ACCESSION   DASRKK010000042 DASRKK010000000
VERSION     DASRKK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /gene="bshC"
                     /locus_tag="VFC15_00890"
     CDS             <1..1602
                     /gene="bshC"
                     /locus_tag="VFC15_00890"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HZM08748.1"
                     /translation="PPDVAHVAAFAKSVPRDRERQARVADALEKQNRAWGASEPTLRN
                     IQRLRDGAFAVVTGQQVGLFGGPLMSLFKAASVLALAGQVEQSGVECVPVFWLATEDH
                     DLDEVNQALLLTHDFQLMPFTAKTAGVTGAPVAHIRFAEGTNELAAEAAKLLGESLAA
                     DYLRESYAEGETFSNTFAKLYTRIFSGREHGLIFLDPADPDLHRIAAPLFVDALHRSA
                     ELDHAVLERNQELRDHGYHEQVKVTPESTPLFALVDGARVPVQRANGTFTIAKKSVSA
                     EELQKRIETAPENFSANVLLRPVLQDYWLPTLAYIGGPAEIAYFAQAAVVYEKLLGRV
                     TPILPRMSATLIEPRIERLLNKYEVELPELFHGECQLRDCLATRSLPAELKQDFEQTR
                     LAVESSMQRVSQSLQTLDPTLVEAGGRAVSKMHYQVNRLEKRAAQAELRRTEVLSRHA
                     AQMENALYPNKSLQEREIAGIYFYANHGPELINRLIELAQARCPEHKVLRLGGPQTDE
                     TTSDVSRRLPRIFIRDENQARRSLR"
BASE COUNT          439 a          640 c          609 g          379 t
ORIGIN      
        1 ccgccggatg tggcgcatgt cgccgcattc gccaagtccg tcccccgcga tcgcgaacgg
       61 caagcccgcg tggccgacgc gctggagaaa cagaaccgcg cctggggagc ttccgagccg
      121 acgctgcgca acattcagcg gctgcgagac ggcgcttttg ccgtggtcac cggccagcag
      181 gttggacttt tcggcggccc gctgatgtcg ctgttcaaag ccgcgtcagt gctggcgttg
      241 gccggacagg ttgaacaatc aggcgtggaa tgtgtgccgg tgttctggct cgccactgag
      301 gaccacgatc tcgacgaagt caaccaggcg ctgctgctga cgcacgattt tcaacttatg
      361 cctttcaccg cgaagacggc aggtgtgacg ggcgctccag tcgcgcacat tcgcttcgct
      421 gaaggaacca acgaactcgc cgcggaggcc gccaagctgc tgggcgaatc gctggccgcc
      481 gattacctgc gcgagagcta cgccgagggc gagactttct ccaacacgtt cgccaaactt
      541 tacacgcgca tcttcagtgg gcgcgagcac gggcttatct tcctcgatcc cgctgacccg
      601 gatctgcatc gcattgccgc accgctgttc gtggacgcgc tgcaccgctc cgccgaactc
      661 gaccatgcgg tgctggagcg caaccaggag ttgcgcgacc acggctatca cgagcaggtg
      721 aaggtcacgc ccgaatccac tcctttgttt gcgctggtgg atggagcgcg cgtcccggtg
      781 cagcgtgcga atgggacttt caccattgcg aagaagtccg tttccgccga ggagttgcag
      841 aagcgcatcg agacggcgcc ggagaacttc agcgccaacg tcctgcttcg tcccgtgctt
      901 caggattact ggctccccac gctggcctac atcggcggtc cggccgagat cgcgtacttc
      961 gcgcaggccg cggttgtgta cgagaagctg ctgggcagag ttactcccat ccttccgcgc
     1021 atgagcgcga cgttgatcga gccgcgcatt gagcggctgc ttaacaagta cgaagtggag
     1081 ctgccggaac tgtttcatgg cgaatgccag cttcgcgact gtctagccac ccgctcactg
     1141 cccgccgaac tcaagcagga tttcgagcag acaagactgg ccgttgagag ttccatgcag
     1201 cgagttagcc agtcactgca gacgctcgat cccacgctag tcgaggcggg cgggcgcgcc
     1261 gtcagcaaaa tgcactacca ggtaaaccgg ctagagaagc gtgctgcgca ggccgaattg
     1321 cgccggacgg aggtcctgtc gcgacacgcc gcgcaaatgg agaacgctct ttatcccaac
     1381 aagtcgcttc aagagcgcga gatcgcaggc atctacttct acgccaacca cggtccagag
     1441 ctgatcaatc gcttgatcga gctggcccaa gcccgctgcc ccgaacacaa agttctgcgg
     1501 ctgggtgggc cgcagaccga cgagacgact tccgacgtct cccgacgtct ccctaggatc
     1561 ttcataaggg atgaaaatca agcacggaga tcactgcgat aacggagaaa actcgataga
     1621 aaccaaacct ccgtgtactc cgtgtctccg tggtgaatga ttttcgacgg agcggagcgc
     1681 ccgcactacc aaggtacaac cactctggaa tgtctaaggc acgcaagagc cattgacttc
     1741 cattccaaat gaccttagag ttgccgcact tatctttcgt aatgatcctg atttggggcg
     1801 gcaagaagca atagtccagg cagtgcggag catcgtgtct aggcgacgtc ccccgtgacg
     1861 aaaacgctct gccggcaatc ttcccactac cccgttcaca atgttgcaat caacggatcg
     1921 ggaaacactt cccggccaga aaaggacaaa taacatggct gattcgcagg actcgaacga
     1981 cgcgggaacg aacactgacg gaggcgcggc gcctgcagct ccaaacctgg catttggggg
     2041 agatggaacg attgtccccg acaaggc
//
LOCUS       DASRKK010000043         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_588721, whole genome shotgun sequence.
ACCESSION   DASRKK010000043 DASRKK010000000
VERSION     DASRKK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588721"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFC15_00895"
     CDS             <1..1127
                     /locus_tag="VFC15_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM08749.1"
                     /translation="HSKGLVFLMKKNKVDVVRGYGKLTGPAKDGIHTVALEGGELKSV
                     KAKNVILCTGSSARMLPGLQPDMQVLTNIEVLSLDQIPKSLIVIGSGAVGVEFASIYK
                     SFGADVTILEMLPRYVPFEDEEVSKELMRVYRKRGINSFVNAKVEKVEKTKTGVAVTF
                     SVDGKPQKLEAEKVLVAVGRAPMTESIGLEKTKIESERGFVKVDEYMRTAEPGVYAIG
                     DIALGYPLLAHSGAREGIIAVTHIAGKPVKPINLLRIPNATYCRPEIGSVGLTEAKAI
                     EKGYTVKVGKFPFTANSRASIVGQHEGFVKIVTDAKYGEILGVHIIGPNATEMIAESV
                     LALEMECTAEELANIIHAHPTLSESMLDAANAVFGMAINA"
     gene            1317..1742
                     /locus_tag="VFC15_00900"
     CDS             1317..1742
                     /locus_tag="VFC15_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZM08750.1"
                     /translation="MSLLKWCDEGPATVSLQATVEDAIQTMLEKQVGAVAVIDEHGVV
                     AGMFTERDVLAKFALSGRDPKRTPVHELMSPMVEMATEETTPSEALQVMLERHYRHMP
                     IVDDRGKVLGICSIRNILEARVDDLLAELDANHPAGAKA"
     gene            complement(1858..2145)
                     /locus_tag="VFC15_00905"
     CDS             complement(1858..2145)
                     /locus_tag="VFC15_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM08751.1"
                     /translation="MTIRWAKDATRQLAAAHTYVAADSLDAADRLLLQITQAVKQLSS
                     HPRAGREGRVKNTRELVIVNTPYIVAYRIKDESTIQVLAVLHGKRRWPESF"
     gene            complement(2142..2381)
                     /locus_tag="VFC15_00910"
     CDS             complement(2142..2381)
                     /locus_tag="VFC15_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08752.1"
                     /translation="MHKTVSFRTDTKKVKALDALASVQDRDRSYLLNQAVDNYLDLQQ
                     YHIELIEKGIRQADNGELVDHSEVEKMVAKLRRKR"
     gene            2484..3024
                     /gene="hslV"
                     /locus_tag="VFC15_00915"
                     /pseudo
     CDS             2484..3024
                     /gene="hslV"
                     /locus_tag="VFC15_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
     gene            complement(3109..>3402)
                     /locus_tag="VFC15_00920"
     CDS             complement(3109..>3402)
                     /locus_tag="VFC15_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08753.1"
                     /translation="QHHLAASKRVGLTREDWQHLKEGQHGNFSEKEEAALDFAEKLTR
                     DPHSIADADLQSLKLHFSEAEIVDLDLLIGLANLTKRFTDPLGADVEFPEEKI"
BASE COUNT          688 a          985 c         1037 g          692 t
ORIGIN      
        1 aacattccaa gggcctggtg ttcctgatga agaagaacaa ggtggacgtg gtgcgcggct
       61 acggcaagct gaccggcccc gcgaaagatg gcatccacac ggtcgcgctg gaaggcggcg
      121 aactgaagtc ggtcaaggcc aagaacgtca tactttgcac cggttcgtcg gcccgcatgt
      181 tgcccggcct gcaaccggac atgcaggtcc tgaccaacat cgaggtgctg agcctggacc
      241 agatccccaa gtcgctgatc gtgattggct cgggcgcggt gggcgtggag tttgccagca
      301 tctacaagtc cttcggggcg gacgtgacta tcctggagat gctgccgcgt tacgtgccgt
      361 tcgaagacga agaggtctcg aaggagttaa tgcgcgtgta tcgcaagcgc ggcatcaaca
      421 gctttgtgaa cgccaaggtc gagaaggtgg agaagaccaa gacgggcgtc gccgtcacct
      481 tttccgtcga tggcaagccg cagaagctgg aagccgagaa agtactggtc gcggtgggcc
      541 gcgcgcccat gaccgagagc atcggcctcg agaagaccaa gatcgagtcg gagcgcggct
      601 ttgtgaaggt tgacgaatac atgcgcaccg ccgagccggg agtttacgcg attggagata
      661 ttgcgctggg ctacccgctg ctggcgcatt ccggcgcgag ggagggtatt atcgccgtga
      721 cccacattgc cggcaagccg gtcaagccga tcaatctgct gcgcattccc aacgccacct
      781 actgccgtcc cgagatcggc agtgtgggac tcaccgaagc caaggccata gaaaagggct
      841 acactgttaa ggttggcaag tttcccttca ccgcgaactc gcgcgccagc atcgtggggc
      901 agcacgaagg cttcgtcaag attgttaccg acgccaagta cggcgagatc ctgggcgtac
      961 acatcatcgg gccaaacgcg accgagatga tcgctgaatc cgtgctggcg ctggagatgg
     1021 aatgcaccgc cgaagagctg gccaacatta tccatgcgca tcccacattg tcagagtcga
     1081 tgctggatgc ggcgaatgcg gtgtttggca tggcgatcaa tgcgtaaggc cgcttttcgc
     1141 ctttcgcgat tcgcctttcg cttttcgcaa agagtgggat tcagcaatct gccgcccgtc
     1201 tctaccgttt gcctcgtacg ccttagctga ttgctggcag ctatcggcaa aaaaggcgaa
     1261 gggcgaacag cgaacagcgt atgacgagta gagtagttgg aaggacctta acaatcatga
     1321 gtcttctaaa gtggtgtgac gaaggcccgg cgacggtgtc cttgcaagcg acggtggaag
     1381 atgcgatcca gacgatgctg gaaaaacaag tgggcgcagt agccgtcatt gatgagcacg
     1441 gcgtcgtggc gggcatgttt accgaacgcg acgtgctggc gaagttcgcc ctcagcgggc
     1501 gcgatcccaa gcgcacgccg gtgcacgaac tcatgagtcc catggtcgag atggcaaccg
     1561 aagagaccac tcccagcgag gcgcttcagg tcatgctgga acggcactac cgccatatgc
     1621 cgattgtgga cgatcgcggg aaagtcttgg gcatctgctc catccgcaac atcctcgagg
     1681 cgcgggtaga cgacctgctg gcagaactgg atgcgaacca tccggcgggc gcgaaggcct
     1741 aagttttcga aacctgggta gagcggcgct ttagcgccgc gttgccgcga attctaaaaa
     1801 ccgagccggc ttcagccggc ggccctactg ttcagttaca ccagtacctg aagtgtttta
     1861 gaagctctcg ggccatcttc gcttgccatg cagcacagcg agtacctgga tggtgctttc
     1921 gtccttgatg cggtaagcca cgatgtaagg cgtattgaca atgaccagct cgcgtgtgtt
     1981 cttgacccgg ccttcacgcc ccgcgcgagg atgagaactg agctgtttca ccgcctgggt
     2041 gatctggagt agcagccggt cggctgcatc gaggctgtct gcggcgacat aagtgtgcgc
     2101 ggctgcaagc tgccgggtcg catccttcgc ccatcgaatc gtcatctctt gcgccgcagc
     2161 tttgcaacca tcttctcgac ttcgctgtgg tcaaccagct ctccgttatc ggcttggcgg
     2221 atgccctttt cgatcaattc gatgtggtac tgctggaggt cgaggtagtt gtccaccgcc
     2281 tggttcagca agtagctgcg atcgcgatcc tgcacggacg caagggcgtc cagtgctttc
     2341 actttcttcg tgtccgtacg gaagctgacc gtcttgtgca taatgcgtca tattacactg
     2401 caatacgccc gtatgggatg aagattgcgc tccagtgcgc gatcgcgccc gccctcatcc
     2461 gcctgttata attgcgtttg ttcatggcta ataagcgttt gatccgctcc acgactgttc
     2521 tttgcgttcg ccgggaggga aaggtcgtga tggccggaga cggccaagtc acgctcggcg
     2581 aaggcgtcat caagcactcc gcccgcaaga tccgccgcct ttaccaggac aagattctcg
     2641 ccgggtttgc cggttccacc gccgacgcat tctcgctgtt cgcgcgcttc gagtcgaagc
     2701 tggagcagta tcacggcaac atcggccgcg ccgcggtcga gctggccaag gactggcgca
     2761 ccgacaagat gctgcgctcg ctggaggccc tgctgctggt cgccgacgag aaccagacgt
     2821 tcctcattag cggagggcga cgtgattgag cccgacaccg gaatcgccgc cattggcagc
     2881 ggtggcccct atgccattgc ggccgcgacg gcgctcttcg agcacaccga cctgccgccc
     2941 cgccggatcg cggaggaggc gatgaagatt gccggcaaga tttgcatcta caccaacgac
     3001 aaagtgacca tcgaagagct gtaagtcaag tttcccaggc tcaaggttcc gggttcaagc
     3061 tgcgctgggc tcatgttgtt ccgccctgcg ggcctggttc ctgcgcgatc aaatcttctc
     3121 ttccggaaac tccacatccg ctcccaacgg atcggtgaag cgtttcgtca aattcgccaa
     3181 gccaatcagc aggtcgaggt ccacgatctc cgcttccgag aaatgcagct tcaggctttg
     3241 aaggtcagcg tcggcaatgg agtggggatc gcgggtgagc ttctcggcga aatccagtgc
     3301 cgcttcttcc ttctcgctga agttgccatg ctggccttcc ttcaggtgct gccaatcctc
     3361 gcgcgtcagg ccaacgcgct tggacgcggc aagatgatgc tg
//
LOCUS       DASRKK010000044         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1370587, whole genome shotgun sequence.
ACCESSION   DASRKK010000044 DASRKK010000000
VERSION     DASRKK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370587"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFC15_00925"
     CDS             <1..1677
                     /locus_tag="VFC15_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZM08754.1"
                     /translation="PGYGTVLRTTNSLLSKFGIPVGEGVEVSVVLDETAKTPAGMVRV
                     SPGHYPRTLFIPGFEGQPELALKDYWIDQYEVTNRQYRAFVSAGGYQKPEYWKFDFVR
                     DGKKLTRDEAMADFRDGAGRPGPKDWVQGEYPKGQDDYPVSGISWYEAAAYAEFAGKS
                     LPTIYHWNRAAGPTTAAFIVPASNFGGSGVLPVGSKQGIGPWGTNDMAGNVKEWTATE
                     SESGKRYVLGGAWDEPIYMFVDPDAQSPWLRSPNIGFRTVKYTEPDSVPKEAQLAIPW
                     PRRDYSKEKPVSDQLFEAYRSVYSYDKTPLNPTVEQVADEDDWKVEKITYAAAYGNER
                     AIAYLFVPKKAKPPFQTVIFFPGSNALLLRKFTLYPTAALDAVVRSGRAVLYPVYKST
                     YERGDGWESDVPDTSSNWRDHVVMWAKDASRALDYVDSRPDLDHDKIGYYGYSWGAVM
                     GAIIPAVEPRIKANVLALGGMEYSKSLPEVDKINFITRVKQPTLLLNGHYDFFFPVDS
                     SQLPFFNFLGAKKEQKRRVMYETGHNIPRNELIKEVLNWYDLWLGLVQER"
BASE COUNT          462 a          636 c          622 g          392 t
ORIGIN      
        1 ccgggatacg ggacggtact acggaccaca aactcgctgc tctcgaaatt cggcatcccg
       61 gttggcgaag gggtggaggt ttccgtggtg ctcgacgaga ccgcgaaaac gccggcggga
      121 atggtacggg tttcgccggg acactatccc cggacgctgt ttattcccgg cttcgaaggg
      181 cagcccgaac tggcgctgaa ggactactgg atagaccagt acgaggtgac caaccgccaa
      241 tacagggcgt tcgtgagcgc tggtggatac cagaagccgg agtattggaa gttcgacttc
      301 gtccgtgatg gcaagaaact aacccgggac gaagctatgg ccgatttccg cgatggtgca
      361 ggacgtcccg gtccgaaaga ctgggtgcag ggcgaatatc ctaagggaca ggacgactat
      421 cccgtcagcg gcatcagctg gtacgaagcc gcggcctacg ccgagttcgc gggcaagagc
      481 ctcccgacca tttatcactg gaaccgcgcc gccggcccta ctacggcagc attcattgtc
      541 ccggcgagca actttggtgg cagcggcgtc ctgccggtcg gcagcaagca gggcatcggg
      601 ccgtggggca caaatgacat ggccggcaat gtgaaggagt ggaccgccac cgaatccgaa
      661 tccggcaagc gttatgtcct gggcggcgcc tgggacgagc ccatctacat gttcgtcgat
      721 cccgacgcgc aatcgccgtg gctgcgttcg cccaacatcg gcttccgcac tgtgaagtac
      781 acggagcccg attcggtgcc caaggaagca cagctcgcga ttccctggcc gcgtcgcgac
      841 tacagcaagg agaaaccggt cagcgaccag ctcttcgagg cgtaccgcag cgtgtactcg
      901 tacgacaaga cgccgctgaa tcccactgtc gagcaggtgg ccgacgagga cgactggaag
      961 gtcgaaaaga tcacctacgc cgcggcctac ggcaatgagc gcgcgatcgc ctatctgttc
     1021 gtgccgaaga aagccaagcc gccctttcaa acggtcatct tctttcccgg ctccaacgcc
     1081 ctgctcctgc ggaagttcac gctgtatccc accgcggcgc tcgatgccgt ggtgcgcagc
     1141 ggacgcgccg tgttgtaccc cgtctacaag agcacctacg agcgcggcga tggatgggag
     1201 agtgacgtgc ccgatacctc cagcaactgg cgcgaccacg tcgtgatgtg ggcgaaagat
     1261 gcctcgcgtg ctctcgacta cgtcgattcg cgccccgatc tcgaccacga caagatcggc
     1321 tattacggct acagttgggg agcggtcatg ggcgccatca tcccagcggt cgaaccgcgc
     1381 atcaaggcca acgtgctggc cctcggcggc atggaataca gcaagtccct gccggaagtc
     1441 gacaaaatca acttcattac ccgggtgaag cagcccacgc ttctgctcaa tggccattac
     1501 gacttcttct tccccgtcga ttccagccag cttccattct tcaactttct cggtgcaaag
     1561 aaagagcaga agcggcgggt catgtacgag accgggcaca acatcccgcg caacgaactg
     1621 ataaaggaag ttctgaactg gtacgacctg tggctggggt tggtgcaaga acgttaagaa
     1681 gcttcgctgg ctcagtgctt tttcccctga acgtgtcgat ggctgcgtgt cccatcgcga
     1741 ccaccatttc gactcgaagc tctggctggg gccacaaatc tcgacaccca cgtttacggg
     1801 atgacctaca tggctctcag gttatcggca aaccggaagt gatgcgttca tccgctccag
     1861 cggatcaata gcgcgagtcg gcaaagcaca ccgaatcccg ttgcaattgc tcgggtgcac
     1921 attggcaatt tcgccttgcc caatcacccc agtgaatctg gacctctaag tgcttgatgc
     1981 tgcgggcctg aaatcgagcg tttcgcttgc ccataatcag aagatttagg gcaaggctaa
     2041 catccgaaga gggcaagcgg aagcgctgca tcaacggagc aattcaagcc aatacctcat
     2101 tgcgtacagg gt
//
LOCUS       DASRKK010000045         5795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_784426, whole genome shotgun sequence.
ACCESSION   DASRKK010000045 DASRKK010000000
VERSION     DASRKK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784426"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1120..1602)
                     /locus_tag="VFC15_00930"
     CDS             complement(1120..1602)
                     /locus_tag="VFC15_00930"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZM08755.1"
                     /translation="MKETAMKRPIAFLVVIGLFSFAVLAHAADRDDAKALVKRAAAYV
                     KYQGKEKALAEISTPKGMFDKGELYVFAYDLQGVMLAHPKNPALIGQNLIAVPDTEGK
                     LFRKEIVERAQSQGSGWVDYVYLNPETNKQEHKTTYFQKVGDIILCCGVYQDYSHGGD
                     "
     gene            complement(1680..3017)
                     /locus_tag="VFC15_00935"
     CDS             complement(1680..3017)
                     /locus_tag="VFC15_00935"
                     /inference="COORDINATES: protein motif:HMM:NF009587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZM08756.1"
                     /translation="MKGKRIYKNLYFQVLTAIVMGALLGHFYPETGADMKPLGDGFIK
                     LIKMIIAPIIFCTVVTGIAGMEDMKKVGRVGVKALLYFEIVSTLALAIGLVVVSVIRP
                     GAGMNADVSKLDTRALETFTAKAQSHGTVDFLMNIIPNTVVDAFAKGDILQVLFFSIL
                     FGFALSMLGQRGKVAFNVIDEVAHVLFGVVGVIMKVAPIGAFGAMAFTIGKFGLGSLS
                     KLGMLMASFYLTCLLFIFIVLGTIAKLCGFSILKFIKYIKEELLIVLGTSSSESVLPR
                     MMAKLENLGCSKSVVGLVIPTGYSFNLDGTSIYLTMAAVFVAQATNTRLTLIQTLTIL
                     GVLLLSSKGAAGVTGSGFITLAATFAAVPTIPVAGLALILGVDRFMSEARALTNLVGN
                     GVATMVVSRWEGELDKDRMSQVLNRETEAEAEEPEAILEGIAVPDEPAAAGQP"
     gene            3219..4919
                     /locus_tag="VFC15_00940"
     CDS             3219..4919
                     /locus_tag="VFC15_00940"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZM08757.1"
                     /translation="MKYRITAEGILETGLSGFDLINFPLLNKGTAFTEDERTDFALHG
                     LLPPHVGNLEDQAARRLKALRDRKTDFGRYAYLRDLQDTNETLFYAVLVSNIEEMMPL
                     VYTPTVGEGCQRFSEIWRKPRGLFLSYPNRHKIREILEHHRYDAIRAIVVSDGERILG
                     LGDQGAGGMGIPIGKLALYTACAGIHPQVCLPVLLDVGTDNVERLEDPLYVGWRHQRV
                     RGAEYDDFVEAFVSAVIARCPRVLLQWEDFAGINAGRLLERYRDRLCTFNDDIQGTAA
                     VAAGTLLAAINVTGMPLTEQRIVLFGVGSAGIGIATLLLTAMKNAGLSEAEARRHFYA
                     VDKDGLLVEGMPDTRPAQQPFVQPRSAIANWKLDNDHISLLDVVRNAKPTTLIGVSGQ
                     AGAFTEEVIRAMAASVDRPVIFPLSNPTSRSEATPADLLKWTEGRALIGTGSPFAAVN
                     WNGRELPIDQTNNSYIFPGIGLGILSVNARRATDTMFMAAARCLAELSPARRSKSGRL
                     LPPVSELRSVSFAVAKAVARQAINDGVAGPLDEQILESRIRANVWEPVYLPYRFDPCP
                     "
     gene            5587..>5795
                     /locus_tag="VFC15_00945"
     CDS             5587..>5795
                     /locus_tag="VFC15_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08758.1"
                     /translation="MEDPNLTIGPGSDVDSTRGSSPEQVRQPVDERLQKLLDKALYGG
                     GHPSAQKIRNFLNGTWLGEPLHVVL"
BASE COUNT         1264 a         1682 c         1609 g         1240 t
ORIGIN      
        1 cgcggagttt gccgctatgc ttcttttcgt tcttcacctt ttgggtgacc tcctggaaat
       61 cggctcgcga agctgctact tcgccgcctc tctgttttac aacaggattt ccagggtccc
      121 caaagggttc gcttcctcag cccctcttca gcccctcaaa tgctgtgcat agatcgggag
      181 aaaggaaggc gaacagggtt accatcgtgc agttggaaac gatcaatgaa aacttatcga
      241 acgtcgcccg atcaggtaga accgccggta tccctggatg ccccctcacg gacacaccgt
      301 tcagcccttt gatgaaggcc acagtttatt ccccattcgt ggttcataat gggtaagttt
      361 tgcgacgccc cgtgaggcca atgaaatcag ccctttcagg gtctcataac tcagtctcaa
      421 aatcgaatgc acgtcgtcga gttgagccaa gggtttgatc cgcggggctg agcaggctgc
      481 acggtcgcca ctccgccgaa aaatctcaaa tctcagtctc aaaacatcag gctgcgattt
      541 cggctctcat ggtggtaccg ggccgtgaac acgcggagcg cccggagtaa atccgctgta
      601 actgcgaccg ttcaccaacc agaaggatgt atagtcgcct acgcgtggaa gtgtgacgga
      661 cccgagcttt cggtgcgcca catccgcacg tagggcgttt tacgtcaagt tagcgtgccg
      721 tgccgtcacc gctagccgat tcgcgggagt aatctcccac atgagggttt ggggccgact
      781 tcgtgtcgca gaccacaaat ccacaatcat tgttccatac tggcggcctc ccacgccgca
      841 gtcagtatgg tcggtcagtt tggggatgat ggtctcgaag ctggccatgt tccgccggaa
      901 tatgcatgta cgcgagattc cgccggcgac gtcgagggtc tcaacaacaa ggttcgagtg
      961 gtgaccagac gatcccacgg attccgcact tatgaagtgc tgcaaaattg ccctttacta
     1021 catgctgggc gacttgctga acgagaatca gccgactgat tctgctgagg aggccataaa
     1081 ggacagcttc aaagcagggt tttgcggcaa agtattggct caatctcctc catggctgta
     1141 atcctggtac accccgcagc ataaaatgat gtcacccacc ttctgaaagt aagtcgtttt
     1201 atgctcttgt ttattggttt ccggattcag atacacgtaa tcgacccatc cggaaccttg
     1261 tgactgcgcc ctttccacta tctccttgcg gaacagcttg ccttcggtgt cgggaacggc
     1321 gatcaaattt tgtccgatca gtgccgggtt cttggggtga gcaagcataa cgccctgaag
     1381 atcgtacgcg aacacataaa gttcgccttt gtcaaacatc cccttgggcg tgctgatctc
     1441 cgcaagtgct ttctcttttc cctggtactt cacatacgca gccgccctct tcaccagggc
     1501 ctttgcatcg tctctatcgg cagcgtgcgc cagaactgcg aatgaaaaca atccgatcac
     1561 aactaagaat gctatgggcc tcttcatggc tgtttccttc actttgtttc aatttacgaa
     1621 cgactgaaat aacccgcagg ttcattgttc gaaatatagt gacttccagt tgtatctcgt
     1681 caaggctgcc cagctgccgc cggttcgtcc ggcaccgcga tgccctccag aattgcttct
     1741 ggttcttcgg cctcggcttc ggtctctctg ttgagaactt gcgacatcct gtccttgtcg
     1801 agttcgccct cccagcggga aaccaccatc gtcgccaccc cattgccaac caagttggtc
     1861 agggcgcgcg cctcggacat gaagcggtca acgccgagaa tcagagccag gccagccact
     1921 ggaatggtgg gtacggcggc aaaagtggcc gccagggtga taaagccgct gccggtaacg
     1981 ccggccgccc ccttggatga cagcagcagc acacccagaa tggtaagggt ctggatcagg
     2041 gtaagccggg tattggtagc ctgggctacg aagactgccg ccatggtcaa gtagatagag
     2101 gttccgtcaa ggttaaagga gtaccccgtc gggattacca gacctactac ggacttgctg
     2161 cagcccagat tctccagttt ggccatcatg cggggcagga ccgactccga ggaggaggtt
     2221 cccagcacga tcagtaactc ctccttaatg tacttgataa attttaagat gctgaagccg
     2281 cagagtttgg cgatagtacc taggacgatg aagatgaaca gcaggcaggt caggtaaaag
     2341 ctggccatca gcatgcccag cttactcagg gaaccgaggc cgaatttccc gatggtaaaa
     2401 gccatggcgc caaaggcgcc gatgggggca actttcatga taactcccac gacgccgaac
     2461 agcacatgtg caacctcgtc aatcacgttg aaagccactt tcccgcgctg gccgagcatg
     2521 gagagagcaa aaccaaacag gatagaaaag aacagtacct gcagaatgtc gcccttggca
     2581 aaggcatcca ctacagtgtt gggaatgata ttcatcaaga agtcaaccgt gccatgcgac
     2641 tgtgcctttg ccgtgaaggt ttccaacgct cgggtgtcca gcttggagac atcggcgttc
     2701 atgcccgcac ccggccggat cacgctgacc accactaggc cgatcgccag ggccagagtc
     2761 gagacgattt caaaatagag caatgccttg acgccaaccc gccctacctt cttcatgtcc
     2821 tccatgccgg cgatgccggt taccacggta cagaagatga tcggagcgat gatcatcttg
     2881 atcagcttga tgaagccatc gcccagcggt ttcatgtccg cgccggtttc ggggtagaaa
     2941 tgcccgagca gagcacccat gacgatcgcc gtcaatacct ggaagtacaa attcttataa
     3001 attcttttcc ctttcatacg tatccttcca cgtcattgat atgattcaga gtctctaacc
     3061 ggcatctccg ttcttagctg ccaacagccc cacactatat accccgtgag gaactcgagc
     3121 gcacgatttt tccttgcctt gggcgccggg cttgaggcca gggacccgcc aaaaaaggaa
     3181 ctgtggaaca atatggaaac cgctggcggc ggacaggcat gaaatatcgc attacagccg
     3241 agggaattct ggagaccgga ctctccggct tcgacctgat taactttccc ctgctgaaca
     3301 aggggaccgc gttcaccgaa gacgagcgca ccgacttcgc cttgcacgga ctgcttcccc
     3361 cgcatgttgg caatcttgaa gatcaggcgg cgcgccgtct caaggcgctg cgcgaccgta
     3421 aaactgattt cgggcgctat gcctatctgc gggacctgca ggacacgaac gaaaccctgt
     3481 tttacgccgt gctggtgagc aacatcgaag agatgatgcc gctggtgtac acccccaccg
     3541 tcggcgaagg atgccagcgt ttcagcgaga tctggcgcaa gccgcgcgga ctgtttctca
     3601 gttaccccaa ccggcataag attcgcgaga tcctcgagca ccaccgctat gacgcaatcc
     3661 gcgcgatcgt ggtcagcgat ggcgagcgca ttctcgggct tggcgaccag ggagccggag
     3721 gcatgggaat ccccatcggc aagctcgcgc tctataccgc ctgcgctggc attcacccac
     3781 aggtgtgttt gcctgtgttg ctcgatgtcg gtaccgacaa tgtggagcgc ctggaagatc
     3841 ccctctacgt gggatggcgt caccaacgag tacggggcgc cgaatacgac gatttcgtcg
     3901 aagccttcgt cagtgccgtg atcgcacgtt gtccccgtgt gctgttgcaa tgggaggatt
     3961 ttgccggcat caacgcggga cgattgctcg aacgctaccg cgaccggctc tgcaccttta
     4021 acgacgacat ccaagggaca gcggcggtgg ccgctgggac gctgctggcc gcgatcaacg
     4081 ttaccggcat gcccttgacc gagcagcgca ttgtgctgtt cggagtggga tcggccggca
     4141 tcggcatcgc cacgcttctg ctgaccgcga tgaagaatgc gggcctgagc gaagcggaag
     4201 cgcgccgcca cttctatgcg gtcgataaag acggattgct ggtggagggc atgccggaca
     4261 cacgtccggc acagcaacca tttgtgcaac cgcgttctgc gatcgccaat tggaaactgg
     4321 ataacgatca catcagcctg ctcgatgtag tgaggaacgc caaaccaact acgctgattg
     4381 gggtctcggg gcaagctggc gcgttcaccg aagaggtgat tcgcgccatg gctgcctcgg
     4441 tcgacaggcc ggttattttc ccgctgtcaa accctacgtc gcgttcggag gctaccccgg
     4501 cagacttgct gaagtggact gaagggcgcg cgctcatcgg cactggcagc ccgtttgctg
     4561 cggtgaactg gaatggtcgc gaattgccga tcgaccagac gaacaactcg tacatcttcc
     4621 ctggaatagg tctgggtatc ctatcggtaa acgcgcggcg cgccaccgac acgatgttca
     4681 tggctgccgc cagatgtctc gccgaacttt ctcccgcgcg gcgcagtaag tcgggacgat
     4741 tgctgccgcc ggtttcggaa ctgcgctcgg tgtcattcgc cgtggccaag gcggtcgcgc
     4801 ggcaggccat taacgatggc gttgcgggcc cgctcgacga gcaaatcctg gaatcccgca
     4861 tccgcgccaa tgtctgggag ccggtctatc ttccgtaccg cttcgacccc tgcccgtaac
     4921 tccacaaggt cccgaatcgg aaccgaatat cacggcgctg atcatagcga accttcggga
     4981 ttgcgcctgg atcacggggc aacatgaatt tatgtggaac cgggaactgg attgcatggt
     5041 ggttcgcggc cggcgagaca cgaagttccc ccgaagtcgg cttcggggaa gtcacttgga
     5101 cgtgttgtcg gctatgctga gattgggtcc aaacgtgtgg gaagtcatcg tggccacgag
     5161 cggctagtcc tcattttgaa tcgcatccgg tgataacact cggaaacatt gtcccacgcg
     5221 cgtacaagcc agtgcgcgcc aaactctccc gattcggtcc ggtgcaggac agcttggcga
     5281 tcgtcgctga ttcggaattc tcgcggtaca aggacggtac atggagttcg cgaccacggc
     5341 gattttccgg ccacggggac tggcggccac tacacggatg gccggcatac atgcgttgcc
     5401 ctcacttgga ctgttggccc ctagtctgaa acttggtccc tgctggagat gagtgttagc
     5461 agagccttat cgtccagtcc tactagctcg ggagccgcgc acatctggaa aagaaaccgg
     5521 cgaacctcac cgtggcggca tccatatgtg ggtcgcaact gccgcatcgt gtataacgag
     5581 gagcatatgg aagatccaaa cctcaccatt ggcccagggt cggatgtcga ttccactcgc
     5641 ggctcatccc ccgaacaggt ccggcagcct gtggacgaga gactacagaa gttgctcgac
     5701 aaagctctgt atggtggagg ccacccgtcg gcacaaaaga tcaggaattt cttaaatggc
     5761 acgtggctcg gcgagcctct acacgtcgtg ctcaa
//
LOCUS       DASRKK010000046         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1370662, whole genome shotgun sequence.
ACCESSION   DASRKK010000046 DASRKK010000000
VERSION     DASRKK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370662"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..879
                     /locus_tag="VFC15_00950"
     CDS             313..879
                     /locus_tag="VFC15_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM08759.1"
                     /translation="MNLQFSAPGFLMLSASPLTMLAHNSEARNILVFPLQVKDSCLRN
                     FLTERVRSKLVYRPFSAETQFVNKFKSGKRTYNCNVLRLSCSDEMMCGGLNPTALLLQ
                     RPPSLTLSLEEHMREHFRLTPRELEIVHLLFKGLTSKEMAQAMSVSPHTIKTFLRLIM
                     MKMSVSTRSGIIGKVVERSLSAVDYQDS"
     gene            1418..>6129
                     /locus_tag="VFC15_00955"
     CDS             1418..>6129
                     /locus_tag="VFC15_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM08760.1"
                     /translation="MSHLLRVSAFFVLIILCFLAFSQLALANSPMDGPGGPILVISNA
                     SDPYSSYYAEILRTEGLNEFAAMDISLVTPSILSNYDVVILAVNPLTSGQVSMVASWV
                     SGGGNLIAMHPDKQLALLLGLTDASATLANGYLLINTASGPGVGLVNQTIQFHGVSDL
                     YTLNSASSLATLYSDASTPTASPAVTLNSFGAGQAAAFTYDLARSIVWSRQGNPAWSG
                     MERDGSPPIRSDDLFFGASVSDPESDWIDLNKVAIPQADEQQRLLANLIVQMNTRNKP
                     LPRFWYFPRGAKAVVVMTGDDHGNGGTVGRFNTFLADSTPGCSVANWECVRGTSYTYP
                     STPMTDGQAAAFVAQGFEVGVHFTTDCADWTPSTLEAFFADQAISFATNFPSLPAPQT
                     NRTHCIAWSDYDTEPQVELNHGIRFDTNYYYWPPSWVNDRPGMFTGSGMPMRFAKIDG
                     TMIDVYQATTQMTDESGQSFPYTIDTLLDNAIGATGYYGAFTANMHNDSPESDGANAI
                     VASAQARGVPIVTSLQMLQWLDGRNGSSFGNFTWNGNSLSFSISLAANSNGAQAMLPN
                     VSSVGLLTGITLNSTPVTYTTQTIKGVAYAVFTAAAGTYTATFAPDTSPPVISSIGAL
                     ANSSSTATVTWTTDELSSSVVNYGTTSSNLNQQMNTPGLVTSHSVSLSGLTPNTTYYY
                     QVTSTDAFNNTATAPAIPATFETPAASLSDGSTSDFASGTGSCSAVPFISGGEVILTP
                     TVDAEFNGSALPPDWSTNVWSSGGGISFGSGAVTIDGADIASNQSHAPGSSLDFVATF
                     SGQPFQHVGFAADAAFDSPWIIFSTAQSGNALYARIFGNPDVLLPGNWLGSPHHFRID
                     WTASSVSFSIDGTVLSTQNVAVPNNLVLVASDLNPGGGSVSVNWMRLSPYATTCSFTS
                     RVLDAGAPVFWSSMSWTADTPSGTSLAISYRVGNTPSPDSSWTSFQSVAGSGGGLAGS
                     SRYIQYAAALATTDTTRTPALQSVNISYGTGTPPPPVINAQSPSPGATNVPVGTTVSA
                     TFSNPMNPATITTASFRLRANGSPSDVPATVSYASGVATLVPMSNLASSTTYTVTVAG
                     TVADMNGEQLGADVIWTFTTGVVTLTHTDTTVADFSAGSTSCSIVAHTGDGEVVLVPT
                     IGEEFSSTTLPPNWTSTPWTGGTPVVSNGMLTVDGTLFAYNTYYSSGSSLEFVATFGA
                     DTFQHLGFGQALQSLSESWAMFSTFNSGNALYARTNNAGIVSDVQIPGNWIGTSHRYR
                     IDWTATSVVFWIDGTMIHTEPVAISATMRPVVSDYNNNGIVLSVDWLLMTPYTSPCVF
                     DSAVIDAGSAANWETLSWDAVVPAQSALALSYRTGNSNNPTDGTWTSFTPVANSGATL
                     TGISRYIQYEANLSTTDTSRTPVLTDVTVAYILSSGGPQGVITAPAPGSTLNGSSVAF
                     QWMAGSGSSAYWLDIGNVPGGNQYYQSGNLGNVLAAAVNGLPTDGSTVYVTLYSLMDE
                     GGQWLSNAYTYAAYNNASLKGVITTPAPGATLSGSSVTFNWTTGTGATAYWLDAGSVP
                     GGNQYYQSGNLGNVLTTTA"
BASE COUNT         1241 a         1890 c         1653 g         1345 t
ORIGIN      
        1 gctgttttcc ctccttccca caaaccttgg aaatcgatga agccgattcc cacattccca
       61 ccgccgcgac gatgacgagg atgaattaaa tcctcaaaac ccggccggtt aagagatacg
      121 cattctgagg gcaaggtcac actctccagt gacacaggtc accgacagtg ctcttcgatt
      181 cgcagatact cggccctcaa atcgcaagtc gctcgccagg gattctcgga gggatgtaaa
      241 gtgcatgcgg ccaactccgg caattgctgc gggccctttt gctagcgatc ctagatcgac
      301 aggagcaaaa gcgtgaatct acaattttct gcccctggtt tcctgatgct gagtgcatcg
      361 ccactcacga tgcttgccca caactccgag gctcgcaaca tccttgtctt ccctcttcag
      421 gtaaaggatt cctgtttaag aaacttcctt accgaaaggg ttcggtcgaa gctcgtttac
      481 cgcccatttt cggcggaaac gcaattcgtc aataaattca aatcaggaaa acgaacttac
      541 aactgtaacg tcctccgact atcctgctcg gatgagatga tgtgtggtgg cctcaatccc
      601 acagcgctac tgctgcagcg gccaccatcg ctcactctat cgcttgagga acacatgcga
      661 gagcatttcc gtctgacgcc acgggaactt gagatagttc atttgctttt taaaggactg
      721 accagcaagg aaatggcgca agctatgagt gtgagtccgc atactatcaa aaccttcctt
      781 cgtctgatca tgatgaagat gagtgtgtcg acccgctctg gtattatcgg caaagtcgta
      841 gaacgatctt tgtctgctgt agattaccaa gacagttgag aagggccgta gctcatgacc
      901 ctccgctact aacacctatc gtcgcgaacc taacggtgac tctgtcggct tgcccctctc
      961 cttcggttca tgactttctt cctcgcctgt cgttcgagtc ctgcgccgac tgaaggctct
     1021 cgtgcacagt tccagtctat cccccaaatg ggttaacccg ttgtgagtag gtcttacttg
     1081 cgattgatac cgctccgaaa tgatctaagg ttatctctta agtctacaga ttagtgtcgc
     1141 ggcgaaagcg cgcccatcgc gatctcagca ggcgactgtt atgccttcct tatatcgagt
     1201 tctttacctt gtcttagttc tcgcatttag ttgcccgctt acacagcttt cattatcgca
     1261 agctgcgctt ttccgacgaa ttccactgaa gaaagaagtt gcctggccgg cactttttca
     1321 ttgtgggata gagcgagagt ctattcgcgt ttttatctct ctcgccacac cgtcatagac
     1381 aacgtatatc cggaccgata aatcaggagg ctgtaccatg tcgcacctac ttcgagtttc
     1441 cgcgtttttt gtcttaatca tcctgtgctt tctcgctttc tcccaattgg cattggcaaa
     1501 ttcgccgatg gacgggcccg gcgggcctat ccttgtgatt tcgaatgcca gcgaccccta
     1561 tagcagctat tacgcggaga tcctgcgcac cgagggtttg aacgaattcg ctgcaatgga
     1621 tatttcgctg gtcacgccct cgattctgtc gaactatgac gtcgtgatct tggccgtgaa
     1681 tcctctgacc tcggggcaag tctcgatggt cgccagttgg gtcagcgggg gcggcaacct
     1741 cattgcaatg caccccgaca aacagttggc attgctactg ggcctcacgg atgctagtgc
     1801 gacgctcgcc aatggctacc ttttaataaa tacagccagc ggtccgggag tcggcctggt
     1861 aaaccagacg atccagttcc acggcgtttc cgatctctac acgttgaaca gcgccagcag
     1921 cctggcgact ctctactcgg acgccagcac tccgactgcg tcgccggcgg tgacgctgaa
     1981 cagctttggc gcgggccagg cagcggcatt tacctacgat ttggcacggt ccattgtgtg
     2041 gtcgcgtcag ggcaatcccg cctggtcggg catggagcgc gacggttccc cgcccattcg
     2101 ttccgacgac ctcttttttg gcgcctctgt ctcagatccc gagtcggatt ggatcgacct
     2161 caacaaggtc gccatccctc aggcggacga gcagcagcga ttgctggcga acctgattgt
     2221 ccagatgaac accagaaaca agccactacc gcggttctgg tacttccctc gcggtgcgaa
     2281 ggctgtggtg gtgatgactg gcgacgatca cggcaacggc ggaaccgtcg gccgtttcaa
     2341 tacctttctc gccgacagta cgccggggtg ctcggtcgcc aactgggaat gcgtccgcgg
     2401 aacctcttat acatatccca gcacgcccat gaccgacgga caagccgcag catttgtagc
     2461 ccaaggcttc gaagtcggag tgcacttcac cacggattgc gcagactgga ctccctctac
     2521 gctggaggcc ttctttgccg atcaggccat cagctttgcc acgaacttcc ccagccttcc
     2581 tgcgccgcag accaatcgca ctcactgcat cgcgtggagt gactacgata ccgagccgca
     2641 agtagagttg aaccacggta tccgtttcga caccaactac tattactggc cgccgtcgtg
     2701 ggtgaatgac cgcccgggca tgttcacggg gtcaggcatg ccgatgcgct ttgcgaaaat
     2761 agacggcacg atgatcgatg tgtaccaggc caccacccag atgaccgacg agtcgggtca
     2821 gtccttcccg tacaccatcg atactctgtt ggataacgcg attggagcca ccggctacta
     2881 cggcgccttc accgccaaca tgcacaacga cagtcctgag tccgacggag ctaatgccat
     2941 cgtggcttcg gcgcaggcgc gcggcgtgcc gatcgtcacg tcgctgcaga tgttgcaatg
     3001 gctggatggg cgcaacggtt cttcgtttgg caactttacc tggaacggca acagcctgag
     3061 tttcagcatc tctctggctg cgaatagtaa cggtgcccag gcgatgttgc ccaatgtgtc
     3121 ttccgtgggc ctgctgaccg gcattacctt gaacagcact ccggtcacct acacgacaca
     3181 gacgatcaaa ggagttgcct acgcggtgtt caccgcggcg gcgggaacct ataccgcgac
     3241 tttcgctccc gacaccagtc ctccggtgat ctcctctatc ggcgcccttg ctaactccag
     3301 cagcacggcc acagtcacgt ggacgaccga cgagttgtcc tcgtctgtcg tgaactatgg
     3361 cacgacttcc agcaatctga atcagcaaat gaacacgccg ggattggtga cgtcgcacag
     3421 tgtgagtctc agcggtctca cccccaacac cacgtattac tatcaggtga cctcaaccga
     3481 tgcgtttaac aatacagcta cggcgcccgc aatcccggcg acattcgaga cgccggcagc
     3541 ctcgctttct gacggctcaa cctccgactt cgcttcggga accggatcgt gctctgccgt
     3601 acctttcatc agcggtggag aggttatcct tacgcctacc gtggatgcgg agttcaatgg
     3661 ctccgctctg ccgcctgact ggtcgaccaa cgtttggtcg agtggtggcg gcatttcatt
     3721 cggcagcggc gcggtaacca tcgatggggc cgacatcgca agtaaccaga gccacgcacc
     3781 cggatcctcg ctggactttg tggcgacctt cagcggacaa ccgttccagc acgtcggctt
     3841 cgccgccgac gctgcttttg attcgccatg gattatcttc agtaccgccc agagcggcaa
     3901 tgctttgtat gcgcgcatct tcggcaatcc ggacgtgcta ttgccgggta attggctggg
     3961 ctcgccgcat catttccgca tcgactggac cgccagttcg gtcagctttt cgatcgacgg
     4021 cacggtactt tcgacacaga atgtcgcggt accgaacaac ctggtgctgg tggccagcga
     4081 cctcaatccc ggcggcggca gcgtctccgt caactggatg cgtctgagcc cctatgccac
     4141 tacctgctcg ttcacctcgc gcgtgctgga cgccggggct ccggtgttct ggtcgagcat
     4201 gtcatggacg gccgacaccc cgtcgggaac ctcattggcc atatcgtacc gtgtcggtaa
     4261 caccccgtct cccgatagct cgtggacgtc gttccaatcc gtcgcaggct cgggtggcgg
     4321 cttggctggc agttcccgct acatccagta cgcagcggcg ctggcaacaa cggacaccac
     4381 gcgaacgccc gcgttacaga gtgtaaacat ctcctacggc actgggactc ctcctccgcc
     4441 ggtgatcaac gcgcaatctc cgtctcccgg cgcgaccaac gttccggtcg gcacaactgt
     4501 atcggccacg ttcagcaatc ccatgaaccc ggccaccatc acgaccgcga gtttccgctt
     4561 gcgggcgaac ggatccccgt ctgacgtgcc agcgacggta agctacgctt ccggcgtcgc
     4621 gacgctggta cctatgagca atctcgcgtc ctcaaccacc tataccgtga cggtggcagg
     4681 cacggtagct gacatgaatg gcgagcagct gggcgccgat gtcatctgga cattcaccac
     4741 cggtgtggtg acgcttacgc acaccgacac aacggttgcc gacttcagcg cgggctcgac
     4801 aagctgctcc atcgttgccc acactggcga tggcgaggtc gtcctggttc cgacgattgg
     4861 cgaggaattc tctagcacga ccctgccgcc gaattggacc agcactccct ggaccggcgg
     4921 cactcccgtc gtttccaacg gaatgttgac ggtggacggg acgctgtttg cctacaacac
     4981 ttactacagt tctggaagct cgctcgagtt cgtggctact ttcggagccg ataccttcca
     5041 gcacctgggt tttggccagg cgctgcagag tttgtcagag tcttgggcga tgttcagcac
     5101 gttcaatagc ggaaacgcgc tgtatgcgcg caccaataac gccggtatcg tgtcggacgt
     5161 gcaaatcccc ggcaactgga ttggcacctc acaccgctac cgtatcgatt ggaccgccac
     5221 cagcgttgtc ttctggatcg acggcacaat gatccatacg gagccggtcg cgatcagcgc
     5281 cacgatgcgc ccagtagtta gcgattataa caataacggc atcgtgctat cggtggattg
     5341 gctgctgatg acgccttata cgtcgccctg cgttttcgac tcggcagtaa tcgatgccgg
     5401 atccgccgcg aattgggaaa cgctgtcgtg ggatgccgtc gtcccggcgc agagcgcgct
     5461 ggcgctcagc tatcgcaccg gcaacagcaa caatccaact gacggcacgt ggactagctt
     5521 caccccggtg gccaattcgg gagcgacgct aaccggaatt tcccgttaca tccagtacga
     5581 ggccaacctc agcacaacgg acacgagccg gacaccggtg ttgaccgacg tcacagttgc
     5641 ctacatcctt tcctcgggcg ggccccaagg ggtgatcacg gcaccggctc cgggatcgac
     5701 cctcaacggc agcagcgtgg cgttccaatg gatggcaggc tcgggttcgt cggcctattg
     5761 gctggacatc gggaacgttc cgggcggcaa tcagtactat cagtcgggga acctgggcaa
     5821 cgtgctggcc gccgcggtga acgggctgcc taccgatggc agcaccgtct acgtaaccct
     5881 gtactcccta atggatgagg gtggtcagtg gcttagcaat gcttacacct atgctgccta
     5941 caacaacgcc agtctcaaag gggtgatcac gacaccggct ccgggcgcga ccctgagcgg
     6001 cagcagcgtc accttcaact ggacgacggg cactggcgct accgcgtact ggctcgatgc
     6061 cgggagcgtt ccgggcggca accagtacta tcagtcggga aatctgggca atgtgctgac
     6121 gaccacggc
//
LOCUS       DASRKK010000047         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_100199, whole genome shotgun sequence.
ACCESSION   DASRKK010000047 DASRKK010000000
VERSION     DASRKK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100199"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFC15_00960"
     CDS             <1..412
                     /locus_tag="VFC15_00960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZM08761.1"
                     /translation="TGRGAQPSSGAPVGNPQQDRSGGFPPSGDGRNKRSVWPMTTKPF
                     RGAHFAVFPPDLPRTCMLAGCPEGGVVLDPFSGVATTGIVAIQHGRQYIGIELNPQYV
                     ELANRRFEETIQTGASLDDALFPGWSGGIFGTSR"
     gene            662..2233
                     /locus_tag="VFC15_00965"
     CDS             662..2233
                     /locus_tag="VFC15_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08762.1"
                     /translation="MSKEQEVAVIHPEGERELVVVGGPNQGSTVDTYGGSIHVKWDET
                     AAVTPFGQLAFFIEFLKTAGLWDGWVQECPVTYESPNAPAKTDVLGTILLSVLAGHRR
                     YAHITALRFDGVNPQLLGMTKVCSEDSVRRAFSAVDQAECMAWLTRHLRISYEPLLSE
                     SWILDIDSTVKPLYGHQEGAVKGYNPGKPGRPSHVYHTYFAATVRLVLDVEVQAGNQT
                     ASSYAQPEFWNYIDSLPAEARPAFVRGDCDWGTERMMAAAEERKILYLFKLKQRSKVK
                     RLIEQAFSRQDWVSAGQGWKGVEAELMLTGWSRQRRVIVLRRRILDERLLAEKKRLAD
                     TTPDQQLALGFVEIVKDGPLYEYAVLVTSLPDEILSLGQHYRDRADAENNFDELKNQW
                     GWSGFTTHDLKRCQIMARTIALIYNWWSLFTRLAIPEKHAEAITSRPLLLNAVGKQTT
                     HSGQTTVTVTSMHAKAPQMRSALQAISSFLATIRNAAEQLTYPQKWLLVLSRVFCHFL
                     KGRILGQSQFVPETG"
BASE COUNT          487 a          649 c          723 g          458 t
ORIGIN      
        1 caccggccgt ggcgctcagc cgtcaagcgg cgcgccagtc ggcaatccgc aacaggaccg
       61 aagcggaggg tttccgccaa gtggcgacgg acgcaataaa cgctccgtct ggccgatgac
      121 gacaaagccg ttccgaggtg ctcacttcgc cgtgttccca cctgacctgc cgagaacgtg
      181 catgttggct ggatgtcctg aaggtggagt cgtgcttgat ccgttttccg gcgtcgcaac
      241 gacgggcatc gtagcaatac agcatggtcg tcaatacatc gggattgaac tcaacccgca
      301 atacgtcgaa ctcgcaaacc ggcgctttga ggagaccatc cagaccggcg caagcctgga
      361 tgacgcactg ttccctggct ggtcaggcgg gatcttcggg accagtaggt gaccgatatg
      421 tcaggctcga tatcgcccgg tccatggcgt cgtaagcccc gcatggtttg gctgactcat
      481 attcccagag cagtcctaaa cccggaacct gttccagcaa cgggtgaact cgcctgttca
      541 ccgggttcgc atcgccgctg gcgaccttga cgtacagccg gtcgcgctga cggccatgca
      601 taatcctaaa aacggcggtt caagaatagg ttgaattggc aagtagctgg tattgttagc
      661 gatgtccaag gaacaggaag ttgccgtcat tcacccggaa ggtgagcgag aattggtggt
      721 ggtcgggggg ccgaatcagg gcagtacggt ggatacctac ggcggaagca tccacgtgaa
      781 gtgggatgag acggcggcgg taacgccttt cgggcagttg gcatttttca tcgagtttct
      841 gaaaacggca ggcctgtggg acgggtgggt tcaggaatgc cctgtgacct atgagagccc
      901 caacgcaccg gccaagacgg acgttttggg caccattctg ctgtctgttc tggcgggtca
      961 tcggcggtac gcgcatatta cagcgctacg cttcgacggc gtgaatccgc aactgttggg
     1021 gatgaccaag gtctgtagcg aggattcggt gcggcgtgcg tttagtgctg tggaccaggc
     1081 cgaatgcatg gcatggctga cacggcattt acgaatcagc tacgagcctc tattgagtga
     1141 atcctggatt ctggacatcg actccaccgt gaagccgctc tacggccacc aggaaggcgc
     1201 ggtgaaaggc tataaccccg gcaagcctgg gcgtccatcg cacgtgtatc acacctattt
     1261 tgcggccact gttcgactgg tgctggatgt ggaagtgcag gccggcaatc agacggcgtc
     1321 gtcatacgcg caaccggagt tctggaacta catcgatagc ctgccggcgg aggcgcgacc
     1381 ggccttcgtt cgaggcgact gtgactgggg aaccgagagg atgatggccg cagcagagga
     1441 acggaaaatt ctgtatctgt tcaaattgaa gcaacgcagc aaggtcaagc ggctcatcga
     1501 gcaggcattt tcgcgccagg attgggtgtc ggcggggcaa ggctggaaag gcgtcgaggc
     1561 ggagttgatg ctgacgggct ggagccgcca gcggcgggta attgtactgc ggcggcgcat
     1621 cctcgacgaa cgcctcctgg cggaaaagaa gcgcctggcg gataccacac ccgatcagca
     1681 actggccctt ggttttgtcg agatcgtcaa ggatggtccg ctgtatgagt acgcggtgct
     1741 ggtcacctca ctgccggatg agatcttgtc gttgggccag cactaccggg accgggccga
     1801 cgcggagaat aactttgacg agctgaagaa ccagtggggc tggagcggct tcacgacgca
     1861 cgacctgaag cgctgccaaa tcatggcgcg caccatcgcc ttgatctaca actggtggtc
     1921 cctgtttacg cgtttggcaa ttccggaaaa gcatgccgag gccatcacca gccgcccgct
     1981 cttgttgaat gccgtcggca agcagaccac gcacagcggg caaaccaccg tcacggtcac
     2041 cagcatgcac gccaaggctc cccagatgcg ctcggctctg caagcgatca gctcatttct
     2101 ggcgacaatt cgcaatgctg cggagcagtt gacctatcct cagaaatggc tcctcgtctt
     2161 gagccgggtt ttttgtcatt ttctcaaggg cagaatactc ggacagtcgc aattcgtccc
     2221 cgagaccggc tgacaactgc cgtttctagg ataaaagccg gacccgttaa cgccggaccc
     2281 gtcaacgccg cgcgtggggg acttacctgt tccggcg
//
LOCUS       DASRKK010000048         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_295751, whole genome shotgun sequence.
ACCESSION   DASRKK010000048 DASRKK010000000
VERSION     DASRKK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295751"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VFC15_00970"
     CDS             <1..1553
                     /locus_tag="VFC15_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM08763.1"
                     /translation="WQFNDNPWYDLNTIIGTISLISVPTQNELATFTNQVLQNNGIVT
                     ASLGVGPNDRRLDGIGLKAGKITHVIYIVKENRTYDQVLGDIPQGNGDPSLVLFGQNV
                     TPSLHALAQRFVLLDNFFDAGEASGDGWPWSTQSMANEYVIKNLPYNYSGRGRNYDFE
                     GQENGYPAGGFPPTDPYGNLNSQQFPNGLPAIPDVAEAPGHHIWDAVQAAGLFYRNYG
                     FYYTFGVSGVIPDNYPASTGLLPAGKDLGGISDFDYRRYDAAYADSEAPTGYGCPYST
                     ATYGHYNMPSRFSEWNREFHEMLQQDPSGNSVPAFMTVRVMHDHTQGTISGNFTPSAE
                     VADNDYSVGQLVDTISKSVTWQHTAIFVIEDDAQDGPDHVDAHRSTAYVISPYIRQGY
                     VDHTFYNTDSILKTIELLLGVKPLTQYDAIATPILAFDSQPSNGATFNAIKASQDVMC
                     QKVSRLVLAKGNPMRKWAIESAKMNFDIPDSAPSAKLNEVLWKSVKGPNSKMPKIQHG
                     VITAADKD"
     gene            complement(1912..>2386)
                     /locus_tag="VFC15_00975"
     CDS             complement(1912..>2386)
                     /locus_tag="VFC15_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08764.1"
                     /translation="VSLEDGFAEGDWDGWKMVTDELGSKIQLVGDDIFVTNIEIFQRG
                     IDAGVANSILIKLNQIGSVSETIDAINLARQNGYTAVISHRSGETEDTFIADLAVATG
                     AGQIKTGSASRTDRIAKYNQLLRIEEELGPAARFLGLKALNYHGELVGKKAGVRG"
BASE COUNT          540 a          702 c          638 g          506 t
ORIGIN      
        1 agtggcagtt taatgacaat ccctggtacg acctgaacac cattatcggc acgatctctc
       61 tgatttctgt gcccacccaa aacgaattgg ccacctttac caaccaagta ctgcaaaaca
      121 atgggatcgt taccgcgagc ctgggagtcg gccccaacga tcgtcgactg gatgggattg
      181 gtctgaaggc cggcaagatc acgcatgtga tctacatcgt caaggagaac cgcacctacg
      241 atcaagtgct tggcgatata ccgcagggta acggcgaccc gagcctggtg ctgtttggcc
      301 agaacgttac gcccagcctg catgcgctgg cgcagcggtt cgtgttgctg gacaactttt
      361 tcgatgctgg agaagccagt ggcgacggtt ggccatggag cacgcaatcg atggccaatg
      421 aatatgtgat caagaacctt ccgtacaatt acagcgggcg tggccggaat tatgatttcg
      481 agggccagga aaatggctat cccgcaggag gttttcctcc cacagacccg tacggcaacc
      541 tgaactccca acaattcccg aatggccttc cggcaattcc cgatgtcgcc gaggccccag
      601 gccatcacat ctgggacgcg gtgcaggcag ccggactgtt ttatcgcaac tatgggttct
      661 actacacctt cggagtgtca ggtgtgattc ctgacaacta tccggcatcg accggcttat
      721 tacctgctgg caaggatctg ggcggcatca gcgactttga ctaccgccgc tacgatgctg
      781 cttatgccga cagcgaggct ccgacaggct atggctgccc gtatagcacc gctacctacg
      841 gtcattacaa catgcccagc cgcttttccg agtggaatcg cgagttccat gagatgctgc
      901 agcaggatcc gagcggtaac tcggttcctg ccttcatgac tgttcgcgtg atgcacgatc
      961 atacgcaggg tacgatatcc ggtaatttca ccccgtccgc cgaagtggcc gataacgatt
     1021 actcagtggg tcaattggtg gataccatca gcaagagcgt aacttggcag cacaccgcga
     1081 tcttcgtgat tgaggatgat gcccaggacg gtcccgacca cgttgacgcg catcgctcca
     1141 ccgcatacgt catcagcccc tacatcaggc agggttacgt tgatcacacc ttctacaaca
     1201 ctgacagcat actgaagacg attgagttgt tgctcggtgt aaaaccgctt acccaatacg
     1261 acgccatcgc cactccgatc ctggcgttcg actcgcaacc ttcaaacggc gcaacgttta
     1321 acgccatcaa ggcctcgcaa gatgtaatgt gtcaaaaggt gtcgcgattg gtgctggcca
     1381 aggggaatcc catgcgcaag tgggcgatcg aatcagccaa gatgaacttc gacatcccgg
     1441 acagcgcacc atccgcaaag ctgaatgagg tgctttggaa gtcggttaag ggaccgaatt
     1501 cgaagatgcc caagatacaa cacggggtta tcacggccgc ggacaaagac taagcagtcg
     1561 gcgcagttaa acggcctcac aggcaacgat cgaggcagac aagcagtaac atcgaataga
     1621 gacgggatcc ggcagagcta gccgggtccc ctttctgttt ggcgcgatgg agagtccttc
     1681 aggctcatct tgtattggaa aagcccatgg actccgcttg ggtaagattg gataattgag
     1741 cgcactggct tcgtagccac cttcgggtga tcccttcaca ggaaagatga tctcggagcc
     1801 cttcatcgcg gtgattatag cgctgccccc cgaaccgcaa atccctccac tcggttgaca
     1861 actctggggt ttgcaacttg aaacccgaaa ctatccacta tccactgacc cctaacccct
     1921 aacccctgcc ttcttcccca ccagctcccc gtgataattc agcgccttca accccaggaa
     1981 ccgcgcggct gggccaagtt cttcctcgat gcgcagcaac tggttgtact tggcgatgcg
     2041 atcggtgcgc gaggctgagc cggtcttgat ctgccctgct cccgtggcga cggcaagatc
     2101 ggcgatgaac gtgtcctcgg tctcgcccga acgatgcgag atgaccgcgg tgtagccgtt
     2161 ctggcgcgcc agattgatgg cgtcaatggt ctcgctgacc gagccaatct ggttcagctt
     2221 gatgaggatg gagttggcca cgccggcgtc gataccgcgc tggaaaatct cgatgttggt
     2281 cacgaagatg tcgtcgccga ccagctggat cttgctgccc aattcgtcgg tcaccatctt
     2341 ccacccgtcc cagtctccct cggcaaatcc atcctcgagt gacacg
//
LOCUS       DASRKK010000049         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_2662, whole genome shotgun sequence.
ACCESSION   DASRKK010000049 DASRKK010000000
VERSION     DASRKK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1766)
                     /locus_tag="VFC15_00980"
     CDS             complement(297..1766)
                     /locus_tag="VFC15_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM08765.1"
                     /translation="MKTPLAASSATDCNAQSLLFQDLGSRTVVADFSGGTLSTDAGAL
                     LLGQVDANLGLTQRLAQCFDDGRNQVWVEHSVQQMLAQRIYGLALGYEDINDHQRLRL
                     DPLLATACNKTDPLGEQRINPADRGIALAAPATLNRLELSNNRSTRCHKVPHDPAKIE
                     ACLLAMGTHCLPKHSQEVVVDLDAMGHRLHGSQEGRHFNAYYDDYCYLPLYAFVGDFP
                     LWAQLRTADHEAAHGVVPALEKIVAAIRQRCRKARIIVRGDSGFCREEIMAWCEGQSE
                     VYYCLGLAKNSVLVEKLEPAMVDARIRHCLSGAASVRAFAEFEYRTHDSWSRPRRVIG
                     KAEVMAEGENPRFVVTNLPAKGFKGDKESRARFTPARLYEELYCARGNMENMLKQQVL
                     DLQADRMSTQYLASNQFRMWESTFAYLLMERLRTQGLAGTQLERATAGSLRLKLLKVA
                     AQVRVSVRRVYVQMSSTYPLQELFRLCHQRLMRLAVPAG"
BASE COUNT          480 a          703 c          647 g          468 t
ORIGIN      
        1 catgcgccgt ccggcgcgaa ctcggccatc gcgagttgga tagactccag cgcatcatgc
       61 agggtttcac cggcgagttg cggctccacg tcggcgaggg ctagcccgca tatttcacaa
      121 gagctctaag ttttcccgtt cgacaagggt ttgggggtaa aatcagacca ggcggctgca
      181 tttttccaac tcggcagccc agcggacgca aatacgcttg tgagtcaagc aataagccag
      241 tttctgacga cactgctcct gtggctcttt gaactgcaac ccccatccgc tggctttcag
      301 cctgccggca ccgccagtcg catcaggcgc tgatggcaga gtcgaaacaa ctcttgcaac
      361 ggataggtgc tgctcatttg cacatatacc cgccgcacgc taacgcgcac ttgcgccgcc
      421 accttcaaaa gcttcaagcg caagctgccg gccgtcgccc gttccagctg cgttcctgcc
      481 aatccctggg tgcgcaatcg ctccatcagc aggtaagcaa acgtcgattc ccacattcgg
      541 aactggttgc tggccagata ctgggtgctc attcggtcag cctgtaaatc caacacttgc
      601 tgcttgagca tgttttccat gttcccgcgg gcacagtaaa gctcttcata gagccgggcc
      661 ggggtgaaac gcgctcgact ctctttgtca cccttgaacc ccttggccgg cagattggtt
      721 accacaaagc gcggattctc cccctccgcc atcacttcgg ctttgccaat gactcggcgt
      781 gggcggctcc aactgtcgtg cgtgcgatac tcaaactcgg caaacgctcg cacactcgcc
      841 gccccactca gacaatgacg gatccgggcg tcaaccatcg ccggctcaag tttctcgacc
      901 aaaaccgagt tcttcgccaa gcctaagcag tagtaaacct cggactgccc ttcgcaccag
      961 gccataatct cctcccggca aaaaccgcta tccccacgca cgatgatccg cgccttgcgg
     1021 caacgctggc gaatggccgc cacgatcttc tccagcgccg gtactacccc gtgggccgcc
     1081 tcatggtcag cggtgcgcag ttgcgcccac aagggaaagt cacctacaaa ggcgtagagc
     1141 ggcagataac aataatcgtc ataataggcg ttgaagtgcc ggccctcttg tgagccgtgc
     1201 agccgatggc ccatcgcgtc cagatccacc acgacttcct gactgtgctt gggcagacaa
     1261 tgagttccca tggccagcag gcaagcctcg atcttggctg gatcgtgcgg caccttgtgg
     1321 cagcgagtgc ttcggttgtt ggacaactcc aagcggttca gcgtggccgg cgccgccagg
     1381 gcaatgccgc gatcggcggg attgatccgt tgctctccca agggatcggt cttgttgcag
     1441 gcggtggcca gcagggggtc caaccgcagc cgctggtgat cattaatgtc ttcataaccc
     1501 agggctaatc cgtaaatgcg ttgagccagc atctgctgga cggaatgctc gacccagacc
     1561 tggttgcgcc catcgtcaaa gcactgcgcc agtctctggg tcagcccaag attggcgtcc
     1621 acctggccca gtagcaaagc cccggcgtcg gtagagagcg tgcctccgga gaaatcagct
     1681 acgacggtgc gagaacctag atcttgaaac aggagcgatt gggcattaca gtctgtcgca
     1741 gaagaagcgg cgagtggcgt tttcatttgg ctgagtgata tcaagccttc tgacgctctc
     1801 gccgcttctt tgttcaacgc aatcgtgata tatccaggtt agtgatagcc acatgccgcg
     1861 cataagagtg ggatgtctga gaaagaccct gccgctggtg gcggcctgtt cgtggcaaaa
     1921 gcagagcttt gttgagagcg aaatgaactt gagtccagta aatgaatttg gcctgagagc
     1981 gttggagcgc tcaaaggcca ataggaatct cgggcgggaa gaatccacgc cagagacgct
     2041 agcttgttac agcacttcta cattaggcgg tgcgctggcg gtaagcaagg gttcttagaa
     2101 agaccagcgc attatgcccc ctccataatc taacgaagac tcccctgatc cgggcggcgt
     2161 acccaagcgg gcgacgcgct tgcgatcagc gggcgggagg cggagttcat cgagcccggc
     2221 cagcccgtca cgccgctggg gcagccccca ggcggctcat gtatcgcgtg ccgctgttcg
     2281 cggcgcggat ttgaactc
//
LOCUS       DASRKK010000050         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_198201, whole genome shotgun sequence.
ACCESSION   DASRKK010000050 DASRKK010000000
VERSION     DASRKK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198201"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..571
                     /gene="clpP"
                     /locus_tag="VFC15_00985"
     CDS             2..571
                     /gene="clpP"
                     /locus_tag="VFC15_00985"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZM08766.1"
                     /translation="MIEQTSRGERAYDIYSRLLRDNIIFLGTPIDDNVANLIIAQMLF
                     LEAEDPEKDISLYVNSPGGSITAGMAIYDTMQFIRPDVITICIGQAASMGATLLAGGA
                     PKKRFALPNSRILIHQPLMSGLSGQATDIDIHAREILRMREITNQILAKHTGQRLEKV
                     ERDVERDFIMNAEQGKEYGIIDDIIFKHK"
     gene            866..2137
                     /gene="clpX"
                     /locus_tag="VFC15_00990"
     CDS             866..2137
                     /gene="clpX"
                     /locus_tag="VFC15_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZM08767.1"
                     /translation="MRTRTGSDEVLRCSFCHKSQDAVAKLISSPTDYPRAYICDECVA
                     VCNSILEDDRSETETTVAPHQLPKPSEVKTFLDEYVIGQHQTKKKLAVAVYNHYKRVY
                     MNRQRGGDVELAKSNILLIGPTGTGKTLLAQTLAKMLDVPFAIVDATTLTEAGYVGED
                     VENIILKLLQAADGDVARAQTGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKIL
                     EGTVANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLERTIARRMGKRSMGFRTDE
                     AKEAAFTECNKRDTQLLAQAEPQDLIKFGLIPEFVGRLPVMGVLNDLDEDALVEILSK
                     PKNAIIKQYQRLFEFENVKLRFTEEALRCIAREAMQRKVGARGLRMILEELMLDLMYN
                     LPSQKRVKEFEVTKEMVENRNVNFPVMEKAG"
     gene            2406..4805
                     /gene="lon"
                     /locus_tag="VFC15_00995"
     CDS             2406..4805
                     /gene="lon"
                     /locus_tag="VFC15_00995"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZM08768.1"
                     /translation="MTQREKFETKKLPMMPIRDVVIFPFMMTPFVVGRESSVRALEEA
                     LAGDKKIFLATQHDASIDEPKPNEIYQVGTIVNIVQSLKLPDGNIKVLVEGLERGKVL
                     QVVDTDGFFEATVRTAKYGTELTPVIEASMQRVTGLFEQYVKLCQSLNYETMIAAVRN
                     DDPSKLTDTIAANLQLAIEEKQELLEIFDPAERLSRIGDVLDVEIEKLNMDRSIQSRV
                     KRQMERAQKEYYLNEKIKAIQKELGRGEKSEFDELKKKIDAAGMPRETHEKAIQELKK
                     LEAMPPMSAESTVSRNYLDWLLAVPWKKRSKEIRNIDVAEKVLNEDHYGLEKIKERIL
                     EFLAVRQLVKNPKGSILCFVGPPGVGKTSLGMSIAKATGRKFVRMSLGGVRDEAEVRG
                     HRRTYIGALPGQIVQMMKKAGTKNPVFMLDEVDKMSMDFRGDPSAALLEVLDPEQNFM
                     FVDHYLDVEYDLSQVFFIATANVLHTIPPALQDRMEVLRLHGYTEPEKVEIAKQYLVR
                     KQREQTGLTEANIVFTDEALTTVIRNYTREAGVRNLEREIGNICRKVARKVVKEGDKY
                     SVTLAAENVSEYLGVLKFRDTEAHERSEVGLVTGLAWTEAGGSILTTEVATVDGKGKL
                     TLTGKLGDVMQESAQAAMSYVRSRAHRLGLPRDFYRNLDIHVHVPEGAIPKDGPSAGI
                     TMATAIASALSHIPVRRDIAMTGEITLRGKVLPIGGLKEKLLAAHRAGILEVILPADN
                     EKDLPEVPENLRAAMKLRFVKTMDDVLAIALERPLPDVPDEAPGLGALSPASEAPTAH
                     Q"
BASE COUNT         1173 a         1458 c         1458 g          986 t
ORIGIN      
        1 ggtgattgag caaaccagcc gtggcgagcg ggcgtacgac atttattccc gcctgctgcg
       61 ggacaacatc atctttcttg gcactcctat cgatgacaat gtcgcgaacc tgatcatcgc
      121 gcagatgctg tttctggaag ccgaagaccc ggaaaaagac atctcgcttt acgttaattc
      181 ccccggcggt tccatcacgg ccggaatggc aatctatgac accatgcagt tcatccgtcc
      241 cgacgtgatc acgatttgca ttggtcaggc ggccagtatg ggtgcgacgt tgctggcagg
      301 tggcgcgccg aagaagcgct ttgcgctgcc caactcacgc atcctgatcc accagccttt
      361 gatgtctggc ttgtcgggac aggctacgga catcgacatt catgcccgcg agattttgcg
      421 catgcgtgaa attaccaatc agattctggc caagcacacc ggccagcgac tggaaaaggt
      481 cgagcgcgat gtagagcgcg acttcatcat gaatgcggaa caaggcaagg aatacggaat
      541 catcgacgac attattttca agcacaagta ggtagtttga attctgaact ggtccgggat
      601 tcgcaccacg gctgtgcgca agaattacca gcgtgattca aaagttgtat ttgaattcgt
      661 ggtgccggca cgcacagccg ttacagttgt gcttcaataa agcataagct taaggggaat
      721 cgcgagagcg ccaatcgcga cgtctgaaac gatggtagtt ctcacaggag cggcacggct
      781 cctgccgggt tcacgcggcc gggaaataca gttcggagac atcgagtctt gactgagagc
      841 gtgcagtgca aaggagtctg ttaccgtgag aaccaggact ggaagcgacg aggtccttcg
      901 ctgttcgttc tgccataaat cgcaggacgc tgttgccaag ctcatttcgt ctcccaccga
      961 ttacccgcgc gcctatatct gcgacgaatg cgtcgcggtt tgcaattcga tcctcgaaga
     1021 cgatcgctcc gaaaccgaga cgaccgtcgc gccgcaccag cttcccaagc cctccgaggt
     1081 caagacgttt ctcgatgagt acgtaatcgg tcagcaccag acgaagaaaa agctcgcggt
     1141 tgcggtttac aaccattaca aacgtgtgta catgaaccgc cagcgcggcg gcgatgttga
     1201 gctcgccaaa tcaaacatcc tgctgatcgg tccgaccgga accggcaaga cgctgctggc
     1261 ccagaccctg gccaaaatgc tggacgttcc gtttgccatc gtggatgcca caactctcac
     1321 ggaagccggc tacgtcggcg aggatgtgga gaacatcatc ctgaagttgc tgcaggccgc
     1381 ggatggcgat gtcgcccgcg cccagactgg catcatttat atcgatgaga tcgacaagat
     1441 cggccgcaag gacgagaacc cttccatcac ccgcgacgtt tcgggcgagg gtgttcagca
     1501 ggctctcctg aaaatcctcg aaggcacggt cgccaacgtt ccgccccagg gcggacgcaa
     1561 gcacccgcac caggaattca cacccgtcga taccacgaac attctgttca tttgcggcgg
     1621 agcttttgtg ggtctcgagc gcacgatcgc gcggcgcatg ggcaagcgct ccatgggctt
     1681 ccgcactgac gaagcgaaag aagcggcctt caccgaatgc aacaagcgcg acactcaatt
     1741 gctggcgcaa gcggagccac aggatctcat caagtttggc ttgatcccgg agttcgttgg
     1801 gcggctaccc gtcatgggcg tgctcaatga cctcgacgaa gatgcgctgg ttgaaattct
     1861 gtccaagccg aagaatgcga tcatcaagca ataccagagg ctcttcgagt tcgagaacgt
     1921 gaaactgcgc ttcaccgagg aggcgcttcg gtgcatcgct cgcgaagcca tgcagcgcaa
     1981 agttggcgcg cgcggactgc gcatgatcct ggaagaattg atgctcgacc tcatgtataa
     2041 cctgcccagc cagaagcggg tcaaggagtt cgaggtcacc aaggaaatgg tggagaaccg
     2101 caacgtcaac ttccctgtta tggaaaaggc ggggtgataa ttttcgctgc tagtcggatt
     2161 ttggaacggt gcggcctagg tcgcgccgtt tgtcattgtg ggttcctcct ccccgcttaa
     2221 ggtgggccca gatcgacata ttccaccgcg acttggcggc caagaagctg tttcagcgcg
     2281 tgttatccta gtcaccagtc agccagccgc aagggcatcc tgcactcgga ttccgtgcgg
     2341 cgcggcaatt ttaaccttta cttctccctc agagcagtgg ggctctgaac gacaaggcgg
     2401 ttcctgtgac ccagcgagag aaattcgaga ccaagaaact ccctatgatg ccgatacggg
     2461 acgttgtcat ctttccgttc atgatgacgc cctttgtggt tggccgggag tcgagcgtgc
     2521 gcgccctcga agaagcgttg gccggcgaca aaaagatttt tctcgcaacc cagcatgacg
     2581 ccagcattga cgagcccaaa cccaatgaaa tctaccaggt cggcaccatt gtcaacattg
     2641 tgcagagcct caagctgccc gatggcaaca tcaaggtcct ggtcgagggt ctggagcgcg
     2701 gcaaggtgct gcaggtagtc gataccgacg gcttctttga ggccacggtt cgtaccgcga
     2761 aatacggtac agaattgacc ccagtaatcg aagcttccat gcagcgcgtc accggactct
     2821 ttgagcagta cgtgaagctc tgccagtcgc tcaactacga gaccatgatc gcagccgtac
     2881 gcaacgacga tccgtcgaag ctgaccgaca ccatagcggc gaacctgcaa ctggcgattg
     2941 aagaaaagca ggaactgctg gaaatcttcg atcccgcgga gcgactaagc cgcatcggcg
     3001 atgtgctcga cgtggaaatt gagaagctca acatggaccg ctccatccag tcgcgcgtca
     3061 agcgccagat ggaacgcgcc cagaaagagt actacctcaa cgaaaaaatc aaggccatcc
     3121 agaaagagct gggacgcggc gagaagagcg agttcgacga actgaagaag aagatcgatg
     3181 ctgctggcat gccgcgcgaa actcacgaga aggcaatcca ggaactgaaa aagctggaag
     3241 ccatgcctcc catgtcggcc gagtcgacag tctcgcgcaa ttacctggac tggctgctgg
     3301 cggtgccgtg gaagaagcgc tccaaagaga tccgcaacat cgacgtggcc gagaaggtgc
     3361 tcaacgaaga tcactatggt ctagagaaga tcaaagagcg catccttgaa ttcctggcag
     3421 tccggcaact ggtgaagaac ccgaaggggt caatcctgtg ctttgtcgga cctccgggcg
     3481 tgggcaaaac gtcactcggc atgagcatcg ccaaggctac cggacgcaag ttcgtccgca
     3541 tgtcgctggg cggcgtacgg gacgaggctg aagtccgcgg ccatcgccgc acctacattg
     3601 gcgcgttgcc cggccagatc gtccagatga tgaagaaggc tggcaccaag aacccggtat
     3661 tcatgctgga tgaagtcgac aagatgtcga tggactttcg cggcgacccg tcggcggcat
     3721 tgctcgaggt gctcgatccc gaacagaatt tcatgttcgt cgatcactac ctcgatgtcg
     3781 aatacgacct ctcgcaggtg ttctttatcg ccaccgcgaa cgtgctgcac accattcctc
     3841 cagcgcttca agaccgcatg gaagtgctgc gcctgcatgg ctacaccgaa ccggagaagg
     3901 tcgagatcgc caagcaatac cttgtccgca agcagcgcga acagaccgga ctcacggaag
     3961 cgaacatcgt cttcaccgac gaagcgctga caaccgtgat ccgcaattac acgcgcgaag
     4021 ccggcgtccg caacctggag cgcgagatcg gcaatatttg ccgaaaagtc gcgcgcaagg
     4081 tggtgaagga gggcgacaaa tactcagtca ccctggcggc tgaaaacgtc tccgagtatc
     4141 ttggagtgct caagttccgc gataccgagg cccacgaacg cagcgaggtt ggcctggtca
     4201 ccgggttggc gtggaccgaa gctggcggct cgattctcac cactgaagtc gcgaccgtcg
     4261 atggcaaggg caagctgacg ttgaccggca aactcggcga cgtgatgcag gaatcggcgc
     4321 aggctgccat gtcgtatgtg cgctcgcggg cgcatcgcct gggactgccg cgcgacttct
     4381 atcgcaacct cgacatccac gtgcacgtgc ccgaaggcgc gatccccaag gacggcccgt
     4441 ctgccggcat caccatggcg acggcaatcg caagcgcgtt gagccatatc ccggtgcgac
     4501 gcgatatcgc catgactggc gaaatcacgc ttcgcggcaa ggtgctgccc atcggagggc
     4561 tgaaagagaa gctgctggcg gcacatcgcg ctggcattct cgaggtcata cttccagccg
     4621 ataatgagaa ggacctgccc gaggttccgg agaacctgcg cgccgccatg aagctgcgct
     4681 ttgtgaaaac catggatgac gtgctggcga tcgcgctaga gcgtccacta cctgatgtgc
     4741 cggatgaagc cccggggctg ggagcgcttt ccccggcttc cgaagctcct actgctcacc
     4801 agtaggccgc aaccatgttc aatcgtgagg cccgtgcgcg ggccttttct tatggaagtt
     4861 gcttagcaca gccagtcaca gcgcttcgcc tcactctgct tgcgctttga ttgcgttacg
     4921 gccatagtga atctatgaag gttttgactc gcttcgtcac gtccgctgcc gatccaaagc
     4981 aattccctgc tcccagcgcg ccggagatgg ccttcctggg acgttacaac gttggcaagt
     5041 cgagcttgat caattcgcta gtggcagaca aaatt
//
LOCUS       DASRKK010000051         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_687041, whole genome shotgun sequence.
ACCESSION   DASRKK010000051 DASRKK010000000
VERSION     DASRKK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687041"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFC15_01000"
     CDS             complement(<1..418)
                     /locus_tag="VFC15_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZM08769.1"
                     /translation="MRSIQWLGLSLTAVFLLCGSSVGGSTQPPSALSYAISTAVYTNG
                     VLVTANIPTSTGGAVTSYSISPALPAGLSLSSLTGIVSGTPTVVAAKACYTVTASNRD
                     GSTAATLIITVIDAPPAQALANLGQQITPLAPQNSRF"
     gene            455..646
                     /locus_tag="VFC15_01005"
     CDS             455..646
                     /locus_tag="VFC15_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08770.1"
                     /translation="MARCFATGHTRTAAMGPAVRLNMYRFETSYDLPITTHQPAAPEE
                     KQYLLAVLYKPDVSAEPRL"
     gene            926..1282
                     /locus_tag="VFC15_01010"
     CDS             926..1282
                     /locus_tag="VFC15_01010"
                     /inference="COORDINATES: protein motif:HMM:NF022970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZM08771.1"
                     /translation="MDVRKLKPEPETHLPTLTKAKLAKLIEEAVVDAYTEEEQTVGFL
                     TMIQEHLALPFSANVLGVEVLVEKVDMTRDGRIVAICRRENVRQRIGILDLPLPTPAP
                     AGAEWIAAYYHWRRGF"
     gene            1283..2425
                     /locus_tag="VFC15_01015"
     CDS             1283..2425
                     /locus_tag="VFC15_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08772.1"
                     /translation="MSEYQYYEFRSMDRALTKAEVAALRSISTRAVITTTSFTNHYEW
                     GDLKADPLKLLEKYFDAFLYVSNWGSRQFYLRLPQELIDYKPLKAMLPSGAAQVRKAG
                     NFVIVAYENVSEDDDWDDGTGWMGSLMSLRSDLLRGDLRCLYLGWLLRLQNEEFAGEE
                     LEPTVPAGLAELSAPLHSLIEFLGIDEDLVEVAATASAPLNAGPSRKELAAWIRGLPQ
                     EEKDDTLVTAVSEPGERWRNDLLRRFQRQNKPRTSHAPAEIQRRTAGDLVAAAHSRAE
                     ERARLLNAKRAAEAARQKAEDEANRARYLDQLEQHEAETWDQIAAHIQKRQPNEYGKA
                     VALLTDLRDLAVRRGRAPEFQSALEKLRQAHAAKESFLRRLAKGNL"
BASE COUNT          620 a          740 c          792 g          523 t
ORIGIN      
        1 cgaagcggga attctggggc gctagcggcg taatctgttg gcccaaattg gcgagggcct
       61 gcgccggggg cgcgtcaatc acggtgatga ttaaggtcgc cgctgtgctg ccgtcccggt
      121 tggacgccgt caccgtgtag caggccttgg cggccacgac cgtgggggta ccgctgacga
      181 tgcccgtgag gctgctcaga ctcaggcccg ccggaagggc cgggctaata ctatacgagg
      241 tcaccgcccc gccggtgctg gtcgggatgt tcgccgtgac gagcacccca ttggtataga
      301 ccgctgtact gatagcgtag ctgagtgccg agggcggctg agtggagcca ccaacgctag
      361 atccgcagag aaggaacact gcggtcagag aaagaccaag ccactggatg gatcgcatag
      421 gaacctcctg ctgcatcgtc gctggcacca tgacatggca cggtgtttcg ccactggcca
      481 cacaagaaca gccgcgatgg ggccagcggt acggctgaac atgtatcgct tcgaaaccag
      541 ctatgacctg ccgatcacaa cccaccagcc cgcagcgcca gaggaaaagc agtacctcct
      601 cgctgtcctt tacaagccag atgtgagcgc tgagccgcgc ctttagatcg ctgatactca
      661 cggttctcat actgcgataa tagccatgat gaccatgtcg gtcatcttct tatttatcgg
      721 acctcgattc ccacatccca atgcgggccc gagtgtacgc gcggcggata aagggctaca
      781 gtgccatcgg ctacaggatt tgcgatactt cccaaggggc attctgcccg gcacgataag
      841 tcgtcgttcc agagaagcgc gagacagaat ccttattaga tggagggtta tgacgcaaca
      901 gaatagcaag cccgttgtgg tccacatgga tgtgcggaaa ctaaaacctg agcctgagac
      961 acaccttcct acactcacta aagcgaagct ggctaagctg attgaagaag cggtggtgga
     1021 tgcttacacc gaagaagaac aaacagttgg gttcctcacg atgattcagg aacatcttgc
     1081 tctgcctttc tctgcaaatg ttctcggtgt tgaggttctt gtggagaaag tggacatgac
     1141 acgcgatggc cggattgtgg cgatttgccg gcgcgagaac gtcaggcaga gaatcggaat
     1201 ccttgatctg ccgttgccga cgcctgcgcc agccggtgca gaatggatcg cagcatatta
     1261 ccactggcgc agaggattct aggtgagtga atatcagtat tacgaattcc ggtcgatgga
     1321 ccgggcgctg acaaaagccg aggtggctgc actgcggtcc atctccacgc gcgccgtgat
     1381 taccacgacg agtttcacga atcactacga atggggggac ctgaaggcgg atcctctcaa
     1441 acttctggag aagtatttcg acgcatttct ctatgtttca aactggggat cgcgacagtt
     1501 ctatcttcgc cttccgcagg aattgatcga ctacaagccg cttaaggcca tgctcccaag
     1561 cggcgccgca caagtgcgaa aagctgggaa ctttgtgatc gtcgcgtatg aaaatgtgtc
     1621 cgaggacgac gactgggacg acgggacagg atggatgggg tccctaatgt ctctgcgctc
     1681 tgacctcctg cgtggcgacc ttcgttgtct ttaccttggg tggctcctcc gtttgcaaaa
     1741 tgaagagttt gccggggagg aactggagcc caccgtgcct gccggcttgg ccgaattgtc
     1801 cgctcccctg cattcgctga tcgagttcct tggaatcgac gaagacctcg tcgaagttgc
     1861 tgcaacggcc tcagcaccgc tgaacgccgg cccgagccgg aaggaactgg ccgcgtggat
     1921 tcgaggtctg ccacaagaag agaaggatga cacgctggta accgcggtat cggaacccgg
     1981 tgaacgctgg aggaacgatc ttctgcggcg ctttcagcgg caaaacaagc cacgcacgtc
     2041 tcacgcgccc gccgagatcc aacggcgcac tgctggagat ctcgtggcag cggctcattc
     2101 ccgtgcggaa gaaagggccc ggctactcaa cgcaaagcga gcggcggaag cggcgcggca
     2161 gaaagcagag gacgaggcaa accgtgcgcg gtatctggac caactggagc aacacgaagc
     2221 agagacctgg gatcagattg cggcacatat ccaaaaacgg cagcccaacg aatatggcaa
     2281 ggcagtcgct ctgctgactg acctgcgtga tctcgcagtc cggcgaggcc gtgctcccga
     2341 attccagtca gcactggaga aacttcgcca ggcacacgcg gccaaggaga gctttctgcg
     2401 tcgccttgcg aaggggaatc tgtaagatga tggattgttc catctgccaa aacccataac
     2461 ccgtgacaac agggtgacaa cgcatgacaa cgtggctgaa ttgacattcc cgagctgtcc
     2521 agagtaaatt ttccaagccc ctatttgagt cgagatgtag ttgaccgcct ggacgtcggg
     2581 agatgtcaaa ggtccgcatc ggaacccgcc ctttcaaccg aaaaaccgct tgaggagaaa
     2641 aatgaagctt ttgcgaatgc tcggattcgc taccg
//
LOCUS       DASRKK010000052         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_980305, whole genome shotgun sequence.
ACCESSION   DASRKK010000052 DASRKK010000000
VERSION     DASRKK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980305"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..638
                     /locus_tag="VFC15_01020"
     CDS             309..638
                     /locus_tag="VFC15_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM08773.1"
                     /translation="MYEQSVTQAFGEVSTALAAHQKLAQSIKDQSRSVVAYQESVRLA
                     DIRYDSGLASYFEVVDAQLQLYPAQTASVVYDLERKVALVNLYKALGGGWKLQDTDWS
                     TPPPPHD"
     gene            741..1382
                     /locus_tag="VFC15_01025"
     CDS             741..1382
                     /locus_tag="VFC15_01025"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZM08774.1"
                     /translation="MCEAIKKIAINTGGGDAPGLNAVIHGAVYAARGLGWDIVGIREG
                     YDGMVEPERFPDGGLVELHRSTVREISHLGGTILGTTNRGNPFHRIVEQPDGSKVEMD
                     CSNDLLQRFRDSGIDALIAVGGDGSLTIASQLYGKGLRVVGVPKTIDNDLESTTVTFG
                     FNTAVSFATECIDRLHSTALSHRRIMVGTCPHAFQFSSASTQHRYAETWGKCR"
     gene            complement(1746..2015)
                     /locus_tag="VFC15_01030"
     CDS             complement(1746..2015)
                     /locus_tag="VFC15_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZM08775.1"
                     /translation="MKKPDPEMIDKENPEWTDAMFAKAKPASEVFPDLAAHSEKRKRG
                     QRGPQKKPRKVMISLRLESSTVDAYKAAGRGYQTRMAAVLEKHAK"
     gene            complement(1996..2262)
                     /locus_tag="VFC15_01035"
     CDS             complement(1996..2262)
                     /locus_tag="VFC15_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM08776.1"
                     /translation="MKIEFDQAKNAKNIRERGLPFTLADEFDWTTAMIVSSSRHGEDR
                     FFAIGYIGSRLHALVFTKSGATLRVISLRRAHKTEVKRYEEARS"
     gene            complement(2462..2776)
                     /locus_tag="VFC15_01040"
     CDS             complement(2462..2776)
                     /locus_tag="VFC15_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM08777.1"
                     /translation="MLEKLKAVVWRGKLNCGHCAVPHRLQGGTTKINHRAAGPYCGRW
                     FLHKFRHTYATRHLQDGIDIRTLQQWMGHRDIASTMVYLKGVRNSDIQVRLNKGSLAA
                     FA"
BASE COUNT          809 a         1081 c         1164 g          862 t
ORIGIN      
        1 cgaaggaccc ctatagctgc gagaaatcac agtgtggccg cgtggcgcag cagcaagttt
       61 gagatggctg gcgaccatag gggtccttcg actcctcgca gcgctcgtcg ctcaggatga
      121 caccccaaaa aactacgtgc gctcatgaat aatccaggtt aggcggcgta agtccgcagc
      181 tttctggttt ggtaagttca ggaacccagt ggagcgtcgc cggcggactc gcaggaccgt
      241 tgttcaatgg agggcggttg aaaagccagt atcggtacag ggttgcgtag tgggacgagg
      301 tccgatcctt gtacgaacag tcagtcacgc aagcattcgg agaggtatcg acagctttag
      361 cggcgcatca gaagctcgcc cagtccatca aagatcagag caggtcggtg gtggcatatc
      421 aggagtccgt gcgactcgcc gacattcgtt atgacagcgg gctggctagc tatttcgaag
      481 tggtcgacgc acagttgcag ttatatccgg cccagacggc atcggtcgta tacgacctcg
      541 agcgaaaagt cgcactggtt aacctttata aagcactggg cggtgggtgg aaactgcaag
      601 acaccgactg gtctacacct cctccccctc acgattagct agtattgcga agttcggtgc
      661 aacgcggagt cctatagtcg atgatcgaaa atctctcaaa ttcgtgaatc agaccctgaa
      721 taaaaggggg ggacacgaga atgtgtgaag ccataaagaa gattgcaatc aataccggcg
      781 gcggtgatgc tccagggctc aacgcggtca tacacggcgc ggtatatgca gcccggggac
      841 ttggttggga tatcgtcggc attcgcgaag gttacgacgg aatggtggag cccgaacgct
      901 tcccggacgg cggtctcgtg gaactgcacc gctcgaccgt tcgagagatc tcccatctgg
      961 gcggcaccat cctgggcacc accaaccgcg ggaatccttt tcatcggatt gtcgagcagc
     1021 ctgacggaag caaagtggaa atggactgct ccaatgacct cctgcaacgg tttcgcgaca
     1081 gcggcattga cgcgctgatc gccgtcgggg gtgacggcag tctcaccatc gcgagccaac
     1141 tctatgggaa gggcctgcgt gtcgtcggtg tacctaagac catcgacaat gatctagagt
     1201 ccaccactgt cacttttggc ttcaacacgg cagtatcgtt tgctacagag tgcattgacc
     1261 gcttgcacag cacggcactt tcgcaccggc gcatcatggt cggcacgtgt ccacatgcat
     1321 ttcagttttc aagcgccagt acacaacatc gctatgctga aacttggggt aagtgtcgtt
     1381 gagcagctgc aacatgtata gacgcagcgt ttcaagatga accctgcgga taacatcact
     1441 cagttcgcgt ttcactcgct gcctctgggg ggtagccccc atagaaggcc acggtcggtt
     1501 caggatacgc ttgatggtat caccggcctc ctggctggca agatctgact ccacgtccaa
     1561 gggccttcag ccacagttac cgcgttcatt gcattgccga caaacgcctg cgcgtccgtc
     1621 tatgtcttca gacccctgcc ctgaaaatgc ggggtgaatt gtcgtagtga tcacacgata
     1681 aaaatacaat ccccggaccg gcacctgggt gcgtctggtc cggggatttt ttgcgtttca
     1741 agaaattatt tcgcgtgctt ttccagcacg gcggccatgc gggtctggta accgcggccg
     1801 gcggccttgt acgcatcgac ggtgctgctt tcaaggcgca gcgagatcat aaccttgcgc
     1861 ggcttcttct gagggccgcg ctggccgcgt ttgcgtttct ccgaatgcgc ggcgagatcg
     1921 ggaaagactt cggatgccgg cttggccttg gcaaacatgg cgtcggtcca ctccgggttt
     1981 tccttgtcga tcatctcagg atcgggcttc ttcatagcgt tttacctccg tcttgtgggc
     2041 gcggcgcagg ctgatgaccc gcaacgtcgc gccggatttg gtgaagacca gagcatgaag
     2101 gcggctaccg atataaccga tggcgaaaaa gcggtcttcg ccgtggcggc tggacgatac
     2161 gatcatggcc gtggtccaat cgaattcgtc ggcgagcgtg aacggtaggc ctctttccct
     2221 gatgttcttc gcatttttcg cctggtcgaa ctcgatcttc attatttaat tgtagataca
     2281 aataaatggt cagtcaactg caatcggcag gggtttcgat cacgaattgt tacagacggg
     2341 taagtgcgcc atggttttcg attttaaggc ccaaggggtg gcctctgtgt tttaccccta
     2401 gcgagatggg tagggaacct ttcatccccc tgcccgcccg tccatcttga aacggcaacg
     2461 gtcaggcaaa ggcggcgagg ctgcccttgt tcaggcggac ctggatgtcg ctgttcctga
     2521 cccccttcag gtaaaccatc gtcgaggcga tgtcccggtg gcccatccac tgctgcaggg
     2581 tgcggatgtc gatgccgtcc tgcaggtgcc ttgtggcgta ggtgtgccgg aacttgtgca
     2641 ggaaccaacg gccgcaatac gggccggcgg cgcggtggtt gattttggtg gtgccgccct
     2701 gcaggcggtg gggcacagcg caatggccgc aattgagctt gccgcgccag acgacggcct
     2761 tcagcttttc cagcatggcg ccgtcgggct tgcccgtcgc gctcgggaat agcggctcgt
     2821 ccgggccggc attggcgggg cggaatttct ccagcagggt aatcagcttc tgcgggaccg
     2881 gggacttccc gctcctccct agctcgtcag gcgttgttgg gggataacaa gggggttttc
     2941 agcaaggcgc tttgagaacc atcttgtagg gggagccgcc cgcttctggc ggcgtggggg
     3001 tctccctccc accccggcct ttccccgggg ggaaacggga aaggggcgaa attcctcagc
     3061 ttatttcctg cggggcgggg ggaaggttaa cgaagggtgg tatcccctcc cttggcaagc
     3121 gaaaggcttc gatggccttg agctcgcaaa aaacagtcag atcgggtagt tcttcgcaag
     3181 ccagtcgagt caccgacacc ccgccttcgc ccagcgcaat ggacgcgctc cgcgggagcg
     3241 cgagttcctt taggagagcc tggtgaatgg cctgtatttc gcaaatatgc gttacatgtt
     3301 caatggggac cggctaacgt ttattcgggg tacaactcaa aggctcaagc gctcaggctg
     3361 agctcccgct ggagagatat cggcgctgcc agcgtccggc acctcgggaa aaagggttga
     3421 cccgtggtgc gaattgagtc ttgcgctttc ctaaccagaa tccatctaac cataagcgta
     3481 tgataacgtc ctttatcatt cattatcccg accaaaaaat taccgcagga aaaatgcaga
     3541 tgcgatcgaa ttctgcgaac tcggcgaggc tgtctcggcg atgtttatca aatggtaaag
     3601 catcggcttt gccgaccatg aaggatgaaa cggtagcgga gatcgatgag cacacgagcc
     3661 ccagtcgaaa tcccagctgc attcgcattc gtgaacactg gccggtcggg gcgaggactg
     3721 gcgcggtatt ccgaaggtcg agcctagccg ttgctccccg tttcctctag gagttcctca
     3781 gtagccaaac cgtgaatccg tttccagccc ccgccacatc gcacccaagc gtgcagattt
     3841 cccgcactgg gcgcacctgt ttgcttagtg ccaagagtta tgtggcttat cgtgctggat
     3901 cgactttcgc gcctga
//
LOCUS       DASRKK010000053         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_198525, whole genome shotgun sequence.
ACCESSION   DASRKK010000053 DASRKK010000000
VERSION     DASRKK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198525"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..493
                     /locus_tag="VFC15_01045"
     CDS             317..493
                     /locus_tag="VFC15_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08778.1"
                     /translation="MPLAVMKSLAGHMDSKMTEYYTGIRDNPKVQAVKAIESANPQLL
                     VLLGIESDPDAKIQ"
     gene            553..629
                     /locus_tag="VFC15_01050"
     tRNA            553..629
                     /locus_tag="VFC15_01050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Met,seq:cat)
     gene            1137..1262
                     /locus_tag="VFC15_01055"
     CDS             1137..1262
                     /locus_tag="VFC15_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08779.1"
                     /translation="MNVVALHKNEAIGIVFVCSELNVSPLQRDQFSTTQSGAQGG"
     gene            complement(1856..2134)
                     /locus_tag="VFC15_01060"
     CDS             complement(1856..2134)
                     /locus_tag="VFC15_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM08780.1"
                     /translation="MATGGCNSRKSLEMEWLDLHALTKYAAVSERTIREWIHLSHNAL
                     PAVQVGKKLLVRRSDFDAWLENHRFRPIESINVTGIVNELLSELVETE"
     gene            2729..3280
                     /locus_tag="VFC15_01065"
     CDS             2729..3280
                     /locus_tag="VFC15_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08781.1"
                     /translation="MTDLEANQSGRDHQSVLHVPREETDILYWCPACRSQHTQRLSTV
                     HMSGLSQFSAVTGGIGWAGSPAIGGAWTTGTSQTQLSAIAAPPPKKSYRNGLLLLFLS
                     PLLAAIVAEAAFLIARRVFGTTLIYEQLATISFFGLEIGAIALLLRVFAFNKNIWPRL
                     FREWQTSFLCRRCGRIFIPGAAL"
     gene            3370..3786
                     /locus_tag="VFC15_01070"
     CDS             3370..3786
                     /locus_tag="VFC15_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08782.1"
                     /translation="MVKKADNLPPPDSNAPPWEVLQERILAVLNDFADDNPVVPDALL
                     LTGAGLAYVTFATTRYGPEAFQNVYENLRSIVQLFQLIIDDERKHLPKRSLKDATNKK
                     KALVERWEVLESVRKKLDILVEQEIRRLQEKPPKPI"
     gene            3901..4278
                     /locus_tag="VFC15_01075"
     CDS             3901..4278
                     /locus_tag="VFC15_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08783.1"
                     /translation="MKNFAALLAITLMLLVVACQKVEAPVPTEKDGEQVWHNLLKKDH
                     GIELLSFKKINGQLEGSHYLLHFEAQVRYLDNNIHDPVAAAGVGIRAGRAGTIETVKA
                     SYEFQKTEKGWQGQDGQLYEFGQ"
     gene            4444..>4740
                     /locus_tag="VFC15_01080"
     CDS             4444..>4740
                     /locus_tag="VFC15_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM08784.1"
                     /translation="MFESSEQVHHALSEVGYLTDSVTATAVYLAAKLKKPLLLEGPAG
                     SGKTELSYAVARAASTQVERLQCYEGITEDKAIGKFDESLQRLYVEMAARSPVAT"
BASE COUNT         1108 a         1268 c         1332 g         1032 t
ORIGIN      
        1 caaaaaggca tttcccaatt gagcgaacac gccgtgtagt ctgcctcgat gaatgtagac
       61 atccgtgccg ttcaaatcgg agagcgactt cataatacgg cgatccaggc tttcaaagac
      121 aaaccacgcg tgctggccgc ccgactcctc gtgagcgacc gccagcccca gttcccgatt
      181 tgacggctgg aacccgaaca gccaccagag aaggtgggct tattcatggc gcaagctgcg
      241 gacgaaggcc ggacttcctg atgtcgagtt ctaccagcta gggcatacca gcatcaccgc
      301 tgggggggag cagaacgtgc cgttggccgt gatgaagagt cttgctgggc acatggacag
      361 caagatgacc gaatattaca ctggtattcg agacaatccg aaagtgcagg cggtcaaagc
      421 catcgaatcg gccaaccctc aactgctcgt cctgctggga atcgaaagcg acccagatgc
      481 taaaatccag taaaacgctc ctttgtggac gtttttgtgg atgattgaca atgcgtgccc
      541 gtaagacctt atggcggtgt agctcaggtg gttagagcga cggtctcata atccgtaggt
      601 cgctggttcg agtccagccg ccgccaccaa ttagaatcaa tgagttgcgg ggattccgcg
      661 tcctcctcaa agatgggcgt ttgcgtcctg tttgcgggtg tttgcgttct gttcgcctgt
      721 gcgttcggca cttgatccag tcgatcaaca aacgagacat tcgcgcccgg gacaaggtgg
      781 ccgtatatat ccacggtcac ctgaattgag ctgtgcccca tttgctcttt cacgtatgtc
      841 agcgtcgcac cgtcttggat cagcaaggag ccgaacgtgt gccgtaaatc gtgcagccta
      901 atgcggcgga gtccagcctt ggccagcacg ggcaggaaat aacggtggaa caggttgtca
      961 ggatccaaga cgtgcccgtc gggcgatggg aagacaaagt catctgcaat gctgtcatga
     1021 ccggcagcga aggcggcgag taggcgcaaa tccctgagct tcagaagaac cgatctcagc
     1081 tgtttcgaca tatccactcg gcgagacttt ttgctcttag gggtcgtgaa ccgccgatga
     1141 acgtagttgc gctgcacaag aatgaagcga ttgggatcgt cttcgtttgc tccgaactga
     1201 atgtctcccc actgcagcgc gaccagttct ccacgacgca gtccggcgcg cagggcggtt
     1261 aagaaaagcg gataatactc agggcagact tctaaagcag catccaagaa ctgctgcact
     1321 tcgattcgcg tgagtgcgac cgcgcgaaag ttcgactttt cgcccttcat gtacttgcca
     1381 agtcgcgcgg ctgggttgct ctccaagatt ccatcttcga ccgcctgagt cagaacacct
     1441 cgaacacagg agtggatgtt tcgcatggtg ttgcgggata ggccacggcc cttagcgacc
     1501 tcggccagaa agctcttcag gcgatctcgg gtcaactgag tcttcgagag cttcagcctg
     1561 ccgaggtctg ggaacacgta ctcgttgagg atgttcctgt atccgcgaac tgtagacggc
     1621 ttacagttca tctccgagta ctcaagcaac caacgttccg cgtattcctg gaaggtcctt
     1681 tcctgatctg gagccccgag gatcttaaga tctcccagcg ccaacttggc ctcgacctgg
     1741 cgacgaattt cctccgccgt gcgcctgtcc gtcccaacac acttacactt gcgcttgccg
     1801 ttccaatcta tgtagaggta ccacttgcct cgtgccctcc gcagcttcac acccattatt
     1861 cagtctccac gagttcagat aatagttcat tcacgattcc ggtgacgttg atcgattcaa
     1921 ttggccggaa gcgatgattc tcgagccatg cgtcgaaatc gctgcggcga acgaggagct
     1981 tcttgccaac ctgcaccgcg ggtaatgcat tatgagagag gtggatccac tcgcggattg
     2041 tacgctccga aacagctgcg tacttcgtca gcgcatggag atcgagccac tccatctcca
     2101 gcgacttccg cgaattgcat ccaccggttg ccatcagtcc ctactgctcc tgcaaggtga
     2161 attccggcgt aggcatatag cgcaaacggc ggatgttgct ctcgcctaat gcgtgggctt
     2221 ggcgccagac aatacgatgt gttcaggatg agaaagctga tcatatggtg aaggctggcg
     2281 aggaaggggc cgatgcttcg cccctttctc gccggcccgg cagaccgatg tcgggtccac
     2341 acgtatttgg tctgcagaac agcactgcag atggtgtgtc gacaattaca agcagattgg
     2401 acgagcgatt tcggtacaga caggatctag aaagccttga gcgcgttccg gctgctcaag
     2461 agacaggtcg atgacagcaa aactatgaga cagttcgcag ccaccggaca agatcgggcg
     2521 acaggtgatc gtctccgcaa acttgttgcg aactactaaa acaccattgc ggggagaaat
     2581 gcaagaaaaa aaattggcag tgataccaaa ttattcccgg cgcgcatgga ccggcaatag
     2641 ctgaatatgt gacgggagaa cttgccaata gcaaagtact tgtcgtaacg taccccacgc
     2701 gatgcggctt cggcaaccag caaatcggat gacagatctg gaagcgaacc aaagcggccg
     2761 agaccaccag tctgtgctgc acgtgccgag ggaagaaacc gacattttgt actggtgtcc
     2821 ggcctgcagg agccagcaca cacaacgact ttcgacggtt cacatgtctg ggttgtcaca
     2881 gttttcggcc gtcacgggag gaattggttg ggctgggtcc cctgcgatcg ggggcgcctg
     2941 gacaacagga acttcccaaa cgcagctatc tgcgattgct gcgccaccgc ctaagaaaag
     3001 ttatcgtaat gggcttctgt tgcttttctt gagcccgctg ctggctgcga tcgtcgccga
     3061 agcggcgttc ttgattgctc ggcgcgtgtt tgggacaacc ctgatttacg aacagcttgc
     3121 aacgatctca ttcttcgggc tcgaaatcgg tgcgatcgcg ttgttgctta gggtctttgc
     3181 ctttaacaag aacatttggc ccagactgtt tcgagagtgg cagacaagtt ttctctgtcg
     3241 gcggtgtggt cgcatcttta ttcccggggc tgctctgtga atgctgcgcg tgctctggag
     3301 aaattattga cgagtaggac cgtctggcct attctcgccc cgactgccta tcgtaggtga
     3361 tatcggattg tggtgaaaaa agctgacaac cttcctcccc ctgactcaaa tgctccgccc
     3421 tgggaagttc ttcaggagcg aatcctcgcg gttctcaatg attttgcgga cgacaatccc
     3481 gtggtgccgg atgcacttct cttgactgga gctgggctcg cgtacgtgac gtttgctact
     3541 acccgatacg gtccagaggc gttccagaac gtgtacgaga atctgcggtc tatcgtgcaa
     3601 ctgtttcaat tgatcatcga cgatgagcga aagcacctgc caaaacgatc gctcaaagac
     3661 gcaacaaaca agaagaaagc ccttgttgag agatgggaag tgcttgaatc agttcgcaag
     3721 aaattggata tcctcgtaga gcaagagatc aggcggctcc aagaaaagcc tcctaagccc
     3781 atctaatgaa agaactctta cgagttgcga ttcgccgagc acttttttat ctcggtttgg
     3841 ataaaaaccg gacggaggaa ccgccatgca gccggctggc cgcgagtgga ggagtacgga
     3901 atgaagaatt tcgcagcact tctggcgatt accctgatgt tgctagtagt cgcctgtcag
     3961 aaagtagaag caccagtccc aaccgagaag gacggcgaac aagtctggca caacctccta
     4021 aaaaaagacc acggaatcga gcttttgtcc ttcaagaaga tcaatgggca attggagggc
     4081 agtcactatc tgctgcattt cgaagcacaa gtgcgatatc tggacaacaa catacatgac
     4141 cccgtcgcgg ctgcaggggt tggcattcgc gcaggtcgcg cggggacgat cgagaccgtc
     4201 aaagcgtcat atgagtttca aaaaacggag aagggctggc agggacagga cggccaatta
     4261 tacgagttcg gacagtgaga gaattctgtc attgaagtca gcccagagcg tccaggtgta
     4321 cgtaggagtg catttccaac acggtaaggc gtcgggacga ctttcacctt ctgaaaattt
     4381 gacgggactc agcggtgccc ctactctgct gcccacactg caacccggtg tgggaggtcg
     4441 aaggtgttcg agtcgtccga gcaggtgcat catgcgctct ccgaggtcgg ttatctcacc
     4501 gacagtgtga ccgcaacggc cgtctatctg gcggctaagc tgaagaaacc gctgctgttg
     4561 gaggggccgg caggaagcgg caagacggag ctctcttacg cggtggcgcg tgcagccagc
     4621 acgcaggtcg aacggctgca gtgctatgaa gggatcaccg aagacaaggc catcggtaaa
     4681 ttcgatgaat ccctgcagcg actttacgtg gagatggccg cccggagccc cgtagcaacc
//
LOCUS       DASRKK010000054         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_393965, whole genome shotgun sequence.
ACCESSION   DASRKK010000054 DASRKK010000000
VERSION     DASRKK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393965"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFC15_01085"
     CDS             complement(<1..516)
                     /locus_tag="VFC15_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08785.1"
                     /translation="MTRFLFWNLNRTDLHGFVSRLAHQENIDVLILAECPTDPAVLLQ
                     TLNANSSDYQYAAGNCGHLLFFTRFESSLLTPLFESHRVSIRHLTLPGRKSILVAAAH
                     LPAKMNFSEESQLVESVHLAQSIDEVETTVGHQRTILLGDLNMNPFEAGMIIAGGLHA
                     VMSRRIAERKTR"
     gene            complement(1834..2256)
                     /locus_tag="VFC15_01090"
     CDS             complement(1834..2256)
                     /locus_tag="VFC15_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM08786.1"
                     /translation="MMTSVDTNVIIALWDRGPAISSAAQSALDAALAHGGLVVGAPVF
                     AELMAAPGRSETFLTSFLKDTGIAIDWNLQEAIWRAAGRSFQEYAARRRGKRDPGPRR
                     ILADFLIGAHASERGYRLLTLDDRLYRTAFPDLALVRA"
     gene            complement(2685..>3713)
                     /locus_tag="VFC15_01095"
     CDS             complement(2685..>3713)
                     /locus_tag="VFC15_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZM08787.1"
                     /translation="LLELCARYGIARVQRAARALLDYSEKMMRAFLATVPPGCYAAED
                     VLDDDGVSDAPVRIAVRITFPRVTKVASRKPRPVIIDFTGSSPQVEGSINAVEAITYS
                     ACFYVFRCLLREDVPATAGLMRPIQLIAPEGTIVNARPPAAVAGGNVETSQRIVDVLL
                     RALAQALPQHIPAASSGTMNNLTIGGMDDRSGKAIPFAYYETIAGGSGASAAHDGVSG
                     VHTHMTNSLNTPAEALEYSYPLRVTKYALRAGSGGEGKHRGGDGIVREIEVLADATVT
                     MLADRRKSRPYGLAGGGDGAPGRTEIIRADGSTELMPSKGSTRLRRGERVRIETPGGG
                     GWGAAEQD"
BASE COUNT          721 a         1093 c         1107 g          792 t
ORIGIN      
        1 gcgggtcttg cgctcggcga tgcggcggct catgacggcg tgcaggccgc cggcgatgat
       61 catgcccgcc tcaaaggggt tcatattgag atcgccgagc agaatggtgc gctgatgacc
      121 aacggtggtc tcgacctcgt cgatgctttg cgccaggtgc acggactcaa ccagctggct
      181 ctcctcggaa aagttcatct tggcggggag atgggcggcg gcgaccagga tgctcttccg
      241 ccccggcagc gtcaggtggc gaatcgagac gcgatggctc tcgaacagcg gcgtcagcaa
      301 actcgactcg aagcgggtga agaaaagcag gtggccgcag ttgccggcag cgtactggta
      361 gtcggacgag ttggcgttaa gtgtctgcag aagcacggcc ggatccgtgg gacactcggc
      421 caggatgagg acgtcgatgt tctcctgatg cgccaggcga ctgacgaagc cgtgtaagtc
      481 ggtccggttc agattccaga agaggaacct cgtcatagct gcagcctagc agaaccgctt
      541 tcccgcatcg agttacgaaa ggacaactca gttgtcagtt gccagtagtc agttgccagt
      601 ttcagattgg tgggaaggaa cgtgggagtg agatgatctg ggtacaccgt tctagccttc
      661 ttttggctag ggcgggaggg aacagtttcg agccgctgac cactaatttg tcatcacgag
      721 cggagtccgc cgcggcgggc ggagtcgagt gacctgcttt tcactgacaa ccggctactg
      781 gctactgaca actgtttttt agggcacaca gatttagggt tttgcctgta tctacgcttg
      841 gcctggggac ctggccccca gctctcaact ttcttcgctc tcctcgtcgc ctaacccttc
      901 ctggtcttcc gctttggcga tctcctcagc ttctggggtg agagtcgcaa tggccgtttt
      961 cttatatgca atgtaggtct tcatgtcttt aaggtcgagg tgtttccgcc gccttggcca
     1021 tgtccttcgt aagatagaac atccagtaat cgtcatacac ttcttccccc agagaagaga
     1081 agggcccggt gccatttcgc aattgctcga tcttgacaac agatcctatg tttttggtgt
     1141 ttccgctgcc aggtcgatcg agccttgtac ccgaatacta cgcgcaagag gagtttctga
     1201 gacaggttcg cctttggacg aagttgagca aactttcgcg cgctcccgag ggcgagttgc
     1261 agaaattagt gcttctgaac accgggatgc ccgcacgatt gtccctgctt cacagtatgg
     1321 cggggctatt cataattaga aggtacaaat agatgtgaaa gcccgggcag aggaaggttc
     1381 gggccggccc gccgaacatt ttcaactcac gatcctggcc gtccactccg actcgatctc
     1441 caccactccg ttctcccgga aagcatagtt gttggttgga gcccccgcat ttatgcgggg
     1501 gaaaagttcg tcttttgatc gcgcgcttta gcgccgggga tcgagaaatc ccgggactga
     1561 agcccatttg aagatggaaa tgctcttccg ctggactgaa gtccagcttc cccctgctga
     1621 agcagggggc ttccacaaaa agtcttaaga gcaggtccct cgactcggac tgaagtcctc
     1681 gctcgggatg acaaagaaca gggactagtg gctaggggtc agggattatt aaagacgcaa
     1741 tccacaagtc ccattcaaga agccgggtgc cccgcccaag cttcccttgc ttgggtggga
     1801 tttcgaaagg gtggacgctg gcttacccca aggttatgcc cgtacaagtg cgaggtcagg
     1861 aaaggcagtc cgatagagac gatcgtcgag cgtgagcagc cgatacccgc gctcggaagc
     1921 gtgagcgcca atcaggaagt cagctaaaat acggcgcggg ccgggatccc gctttcccct
     1981 gcgccgtgcg gcatattcct gaaaagaccg tcccgcagcg cgccaaattg cttcctgtaa
     2041 attccagtcg atggcgatac ccgtgtcctt taagaatgaa gtaaggaacg tctcgctgcg
     2101 gcccggcgcg gccattagct cagcgaagac cggcgcgccg accaccaggc caccgtgggc
     2161 gagcgcggca tccaaagcgg attgtgccgc cgaactgata gcgggaccac ggtcccagag
     2221 agcaatgatg acattggtat cgacggaagt catcatcttc cgcgcgtctc tttggtccaa
     2281 cgattaattc ccgcccggcc ggacgggatt cccggatttc cgattccccg atactttgca
     2341 aatgggcttt ccgtcctcac cggccgtact cggacccccg cttcatcgct ttcaaaaatc
     2401 aactggtcgc ccgatctcac acccagcaca cgacggatct cgtgcggcac ggtaatttgt
     2461 cccttgctcg tgatttttgc ctgcttttgc aaccgtcacc tccaacattc cttactttat
     2521 cacagtgctc cttaccttga atttgaacgg gggaagggga tagcgccggg tgccccaccc
     2581 aagtttctct tgcttgggta gaagtcttgg tggttccgcc ttaacccatc ttccgcgcga
     2641 gcacttgatg ttgtccgccg ggcggcaatt tatcggttga cccctcagtc ctgttccgcc
     2701 gctccccagc cgccgccgcc gggggtttcg atgcgaactc gttcgccgcg ccgcaagcgc
     2761 gtgctgccct tgctcggcat gagttcggta gaaccatcag cgcgaattat ctcggtccgt
     2821 cccggcgctc cgtcgccgcc gcccgccaga ccgtaaggcc gagacttgcg ccggtcagcg
     2881 agcatggtca ccgtggcgtc ggcgagcact tctatttctc gcacgatgcc gtcgccgccg
     2941 cgatgtttgc cttccccgcc gctacccgca cgcagcgcat acttcgtcac cctcaaagga
     3001 taactgtatt ccagcgcttc cgcgggagtg ttgagcgagt tggtcatgtg ggtgtgcacg
     3061 cccgagacgc cgtcgtgggc cgcgctggct cccgagcctc cggcgatggt ttcgtagtac
     3121 gcgaaaggga tggccttccc gctgcgatcg tccatgccgc cgatggtgag attgttcatg
     3181 gtccccgacg acgcggccgg gatgtgctgg ggcagcgcct gggccagcgc gcgcagaagc
     3241 acgtcaacga tgcgctgcga ggtctcaaca tttccgccag ccacggctgc gggcggacgc
     3301 gcattgacga tggtgccctc gggcgcgatc aactgaatgg ggcgcatcaa tccggcggtg
     3361 gcgggaacgt cttcgcgcag caggcagcgg aaaacgtaga agcaggcgga atacgtgatg
     3421 gcttccacgg cgttaatgct gccttccacc tggggcgagc tgccggtgaa atcgatgatg
     3481 acgggtcgcg gcttgcggct cgcgactttt gttacgcgcg gaaaggtgat gcgcacggca
     3541 atgcgcacag gcgcgtcgct gacgccatcg tcatcgagaa catcttcggc ggcgtagcat
     3601 cccggaggca ccgtcgccaa aaatgcgcgc atcatcttct cggagtaatc cagcagcgct
     3661 cgcgcggcac gctgaacgcg cgcgatgccg taacgcgcgc aaagttcgag cag
//
LOCUS       DASRKK010000055         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_198557, whole genome shotgun sequence.
ACCESSION   DASRKK010000055 DASRKK010000000
VERSION     DASRKK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198557"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFC15_01100"
     CDS             complement(<1..723)
                     /locus_tag="VFC15_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM08788.1"
                     /translation="MATTTVAPKTKLTGGSFLLEDHNLENVFTPEDFNEDQQMIVKLA
                     DEFAVNELLPNIEKIEHKDWQLTRDLLKKAAEMGLTNADVPAEYGGSDMDKISSAIIA
                     DQMAKCGSFVVSMGAHAGIGTLPLVFFGTEEQKKKYLPRLASGEIIGAYALSESTSGS
                     DALNSRTRAVLSPDGKHYILNGEKMWITNAHFADLFTIFAKVDGEKFTAFLVEKDFLG
                     FSVGAEEKKMGIRGSSTAPLILN"
     gene            complement(757..1935)
                     /locus_tag="VFC15_01105"
     CDS             complement(757..1935)
                     /locus_tag="VFC15_01105"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZM08789.1"
                     /translation="MREVVIVSAVRTPVGKAYKGTLRATRPDDLAAFAIKGALERVPQ
                     LDPKEIEDVILGCAMPEAEQGMNVARIASLRAGLPIEVSAMTVNRFCSSGLQAIAQGA
                     ERIMAGGAEVVVAGGVESMTMIPMGGNKVSANPWLVTNYPDAYLSMGLTVERVAARCN
                     ITREQADQFSVESHKKAFAAIEAGRFDDEIVPVPVSFTTPNGSKPKRTEIIFKIDEGP
                     RADTSMEALGALKAAFHAKGTVTAGNSSQMSDGAAAAIVMSAERAKALGLKPLLRFVS
                     FATAGYKPEEMGLGPVYAIPKALKLAGLKLSDIDVIELNEAFAAQSLAVIKEGGLDPA
                     RVNPNGGAIALGHPLGCTGAKLTASIVRELKRRGARYGMVTMCVGGGMGAAGIFENLQ
                     "
     gene            complement(2509..2877)
                     /locus_tag="VFC15_01110"
     CDS             complement(2509..2877)
                     /locus_tag="VFC15_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08790.1"
                     /translation="MANYWVVGAMWGGKDDQSETFIRRGYWFLGWGDDEQPAQAALRD
                     QIRPGDRIAIKNMLGQGSSNIEVRALGVVKEIDQDDKRVYVNWLVKRLEREVPGRGCF
                     ASIHGPFHADDPWVKEVFHI"
     gene            complement(2908..>4070)
                     /locus_tag="VFC15_01115"
     CDS             complement(2908..>4070)
                     /locus_tag="VFC15_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZM08791.1"
                     /translation="AANVEKLLASGRTSWYADDPKVASGRKYFDVATGEYLAEVVPAG
                     VWSVTVAKKATGVVKKNAGASLIDIGDGVGCIEFHSKMNALGGDIVSLITQTMKGGGG
                     AENFDAFVITNDAGNFSVGANIMMLLMAAQEEEWDEVDMAIRQFQNMTQAIKFSPKPV
                     VVAPFGMTLGGGTEITLHAHSRRPHAELYAGLVEVGVGLLPGGGGCKEMTLRAIHKAT
                     HIRPQGRGESVELMEAMKQAFEIVALAKVSTSALEAKNYGFISPGELITMNRERVLTD
                     AKTRALELFHEGFRPLTMRTDIPAPGESILATLKMGIHIMRCGEFISDHEVKIATKVA
                     HVLCGGEVNPGTLVSEQYLLDLEREAFISLAGEKKTQERIQFTLKTGKTLRN"
BASE COUNT          755 a         1197 c         1184 g          934 t
ORIGIN      
        1 gttgaggatg agcggcgcgg tggaggagcc gcggatgccc atcttctttt cttcggcgcc
       61 cacggagaag ccgaggaagt ctttctctac caggaaagcg gtgaacttct cgccatcgac
      121 cttggcgaag atggtgaaca gatcggcgaa gtgggcgttg gtgatccaca tcttctcacc
      181 gttgaggatg tagtgcttgc cgtcaggaga caggaccgcg cgagtgcggc tgttgagtgc
      241 gtcggagccg ctagtgcttt ccgacaacgc gtaggcgccg atgatctcgc ccgaagccag
      301 ccggggcaga tacttcttct tctgttcttc ggtgccgaag aagacgagag gcagggtgcc
      361 gatgccggca tgtgcgccca tggagacgac aaagctgccg cacttggcca tctggtcggc
      421 gatgatggcg gaagaaatct tgtccatgtc ggagccgccg tattccgccg gaacgtcggc
      481 gttggtcagg cccatttctg cggccttctt gagcaggtcg cgggtgagct gccagtcttt
      541 atgctcaatt ttttcgatgt tggggaggag ttcgttgacc gcaaactcat cggcgagctt
      601 gacgatcatc tgctggtctt cgttgaagtc ttcgggagtg aaaacgttct ccaggttgtg
      661 gtcttcgagc agaaaacttc cgcccgtaag tttcgtcttg ggagctactg ttgtcgttgc
      721 catggttcaa cctcagttgt aagtctcaaa agtctcttac tgcaaattct cgaagatgcc
      781 cgccgcaccc atgccgccac cgacacacat ggtcaccatg ccgtatcgcg caccgcggcg
      841 cttgagttcg cgcacgatgc tggcggtcag cttggcgccg gtgcatccca ggggatgtcc
      901 cagcgcgatc gctccgccgt tcgggttgac tcgcgccgga tccagtccgc cttccttaat
      961 caccgcgagc gactgtgccg cgaaggcttc attcaactcg attacgtcga tgtcgctcag
     1021 cttgagtccg gccagcttca gcgctttggg aatggcatag accggtccca gccccatctc
     1081 ttcgggcttg tatccggcgg tcgcgaagct cacgaagcgc agcagcggct tcaatcccaa
     1141 cgctttcgca cgctctgcgg acatgactat cgcggccgca gcgccatccg acatctgcga
     1201 cgagtttcct gcggttacgg tgcccttcgc gtggaaagcc gccttgagcg cgccgagcgc
     1261 ttccatcgac gtgtcagcgc ggggaccttc gtcaatcttg aagatgatct ccgtgcgttt
     1321 cggtttgctg ccatttggag tggtgaagct gacgggcaca ggcacaattt cgtcgtcgaa
     1381 tctgcccgcc tcaatggccg cgaaggcttt cttatggctc tccacggaga actggtcggc
     1441 ctgctcgcgc gttatgttgc agcgcgcggc cactcgttcg acggtcaagc ccatcgaaag
     1501 atacgcatca ggatagttcg tgaccagcca ggggttggcc gacaccttgt tgccgcccat
     1561 ggggatcatg gtcatggatt cgacgccgcc cgcgacgacg acttccgctc cacccgccat
     1621 aatgcgttcg gcgccctggg caatcgcctg caggcccgac gaacagaagc ggttgacggt
     1681 catggctgag acctcgatcg gcagacctgc ccgcagcgac gcgatgcggg ctacgttcat
     1741 gccctgctcg gcttcgggca tggcgcaacc gagaatcacg tcctcgattt ccttgggatc
     1801 gagctggggc acgcgctcta gagcgccctt gatggcgaag gcggccaggt catcgggacg
     1861 ggtggcgcgc aaggttcctt tgtaggcttt tcctaccggg gtacgaacgg cactgacgat
     1921 tacgacttca cgcatgagag gctccgtagc tggtgactgg taactggtaa ctggcgggta
     1981 acaagttcgt tgactctgcc ttgcgatgtg tgctgcacaa atcggtcaaa aaacgtgatt
     2041 cacgattgtt tagaatcaat gagttggatg gaaattgttc gcatgagttt gattcaaaaa
     2101 gagttagggg tatccatgtc tcgggtaagg agttacgggc cggattttga actattcgtc
     2161 gcatttgtgc ctgtggcata ttaaccagtt gaccatcaac ttctgtcgcc gctacgcggc
     2221 gcacactctc aaaaccgccc tacccagggc ttacgccatg ggctaaaatt gtgccgccct
     2281 tcggctgatg ggggcgcgct ctctgtcggg cgtcggatta gttcgggtgt tcatcggagt
     2341 ccccgagaaa tgcatctccg acgagtgctt cgcaccaatc gagcgcctct gtctcgtgtt
     2401 gtaaatcccg agacatttct tcgtagacag cttgtaagtc agtggaatcc attttcgttt
     2461 ctgccgccgc tacgcggctc tctcctcggg gagtcctcct cacaaccatc aaatgtgaaa
     2521 cacttccttg acccaaggat cgtcagcgtg aaagggacca tgtatcgacg cgaagcagcc
     2581 tctgcccggt acctcccgtt ctagtcgctt gacaagccag ttcacgtaaa cccgcttgtc
     2641 gtcctgatca atttccttca cgacgccaag cgcacgcacc tcaatgtttg aggatccctg
     2701 acccagcata ttcttgatcg cgatgcgatc acccggacga atctgatcgc gcagtgccgc
     2761 ctgtgccggc tgttcatcat ctccccaacc caggaaccag taacctcttc gaatgaacgt
     2821 ctctgactga tcgtcttttc ctccccacat tgcgccgacc acccaataat ttgccactat
     2881 tcctccagaa gaacaccttt atgggcttca gttcctcagc gtctttcccg ttttcaacgt
     2941 gaactggatg cgctcctggg ttttcttttc gcccgcgagc gagatgaagg cctcgcgctc
     3001 caggtcgagc agatactgct cgcttaccag agtgccgggg ttgacttcgc cgccgcagag
     3061 cacgtgggca accttggtgg cgattttcac ttcgtgatcg ctgatgaact cgccgcagcg
     3121 catgatgtgg attcccatct tcaacgttgc caggatgctt tctccgggcg ccgggatgtc
     3181 ggtgcgcata gtcagcggcc ggaagccttc gtggaagagt tccagcgcgc gggtcttggc
     3241 gtcggtaagg acgcgctcgc ggttcatggt gatgagttca cccggtgaaa tgaagccata
     3301 attcttggct tccaaagcag aggtcgaaac cttcgccaga gcgacgattt cgaaggcctg
     3361 cttcatcgct tccatcagct cgacgctctc gccgcggcct tgcggacgga tgtgtgtcgc
     3421 cttgtggatg gcgcggaggg tcatctcctt gcaacctccg ccgccgggca acaggccgac
     3481 gccgacttcg acgagtccgg catacagctc tgcgtgaggc cggcgagagt gagcatgaag
     3541 ggtgatttcg gtgccaccgc cgagggtcat gccgaagggc gcgaccacta ccggcttggg
     3601 ggagaacttg atggcttgcg tcatgttctg gaactgacgg atggccatgt caacttcgtc
     3661 ccattcttct tcctgggcgg ccatcagcaa catcatgatg ttggcgccga cggagaagtt
     3721 tccggcatcg ttggtgatga cgaaggcgtc gaagttctcg gctccgccgc cacccttcat
     3781 ggtctgggtg atcagcgaaa cgatgtcgcc gcccagcgca ttcatcttgg agtggaattc
     3841 gatgcagccg acaccgtcgc cgatatcgat gagcgaggcc ccggcattct tcttgaccac
     3901 gccggtggct tttttcgcga ccgtgaccga ccaaacacct gcgggaacta cttcggcaag
     3961 gtactcgccg gtagctacgt cgaagtactt gcgtccgctg gcgaccttgg gatcgtcggc
     4021 gtaccagctc gtcctgcccg aagccagcag cttttcgacg ttggcagcga
//
LOCUS       DASRKK010000056         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1175965, whole genome shotgun sequence.
ACCESSION   DASRKK010000056 DASRKK010000000
VERSION     DASRKK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175965"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFC15_01120"
     CDS             <1..378
                     /locus_tag="VFC15_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZM08792.1"
                     /translation="WVPVDGTYDLGTGPRQSSEMQEEAYPLSNCISCCCCMEACPQFN
                     DKTGFVGAATIAQVRLFNSHPTGKALKTERLKALMGDGGIQECGYAQNCVEICPKELP
                     LTKSIADVGGQVMWQAVKDLFRT"
     gene            551..1054
                     /locus_tag="VFC15_01125"
     CDS             551..1054
                     /locus_tag="VFC15_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08793.1"
                     /translation="MAIPHRGTTSSTTYFVTSGTFCKTNILQSDAMAELFCHTLFRYR
                     DAKKFRAHSFVVMPNHFHLILTVAEGLTLERAMQLIKGGFSREAGRRLGARYEIWQKS
                     FVDRRVRDAVELARYTAYIQENPVRAGLVGVAGEYRYSSLNPAFIMDELPQRLKPIFT
                     DARLMHR"
     gene            1260..2615
                     /locus_tag="VFC15_01130"
     CDS             1260..2615
                     /locus_tag="VFC15_01130"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZM08794.1"
                     /translation="MPSRPQVLVSALLFSALAIAQTATPAAKSSQAPPPAKSGSKALT
                     ISPNGAATGWRAIHNSALVVDTHADTPGRFVDENFDLSQDAGTGYLDFNKIKAGNLGA
                     EFFSIWVEPKANKGHETKRALDMIDSVYEQARLHPDKMMMAFSTQDILAAHRQHKLAA
                     LLGVEGGHAIQGDIRILRDYYRLGVRYMTLTWSNTNELGDSSGDLNDKSVEHHNGITD
                     FGRQVVREMNRLGMMVDISHVADRTFFQALVASRAPVIASHSSSRELTNAPRNMTDDM
                     LIALARNGGVAQVNFYCGFISQKYVDREKQLKEQHDPDYEKVQSLFLKAQTPEVKKEL
                     DEAKARLVVKLPRPPLTDLIDHIDHMVKVAGVDHVGLGSDFDGIDCSPQGIDSAADLP
                     KITQALYDRGYKAADLDKILGGNLMRVFAEVEKLAKQIQAETNQDTRREIVPTAQSQQ
                     Q"
BASE COUNT          606 a          772 c          732 g          538 t
ORIGIN      
        1 tgggttccgg ttgacggtac gtacgatctt ggcaccggcc cgcgccagtc gtcggaaatg
       61 caggaagaag cgtatccgct gtccaactgc atctcgtgct gctgctgcat ggaagcctgc
      121 ccgcagttca acgacaagac gggtttcgtg ggcgcagcta ccatagcgca ggtccggctc
      181 ttcaactcgc atcccaccgg caaggcgctc aagactgaac gtctgaaggc cctgatgggc
      241 gacggcggca tccaggaatg cggttacgcg cagaactgcg tcgaaatctg ccccaaagaa
      301 ttgcccttga ccaaatccat cgccgacgtt ggcggccagg tgatgtggca ggccgttaag
      361 gacctgttcc ggacatagag gaaaaacgag gagatagtcg tcgcatatcc agctcttgtc
      421 acagacactg ttacagtgtg tcttgaatag gtaccgagag aattaagacc ccgaaaaagg
      481 gtggagcacc gctttagcgg tgcattggca aagaccctca agaagccggc tttagccgct
      541 gaggtgatcc gtggccatac cacatcgtgg gacaacgagc agtacaacct acttcgttac
      601 ttccggcacc ttctgtaaga cgaacattct tcagtctgac gcgatggcag agctattctg
      661 ccatacgctt tttcgctatc gggatgcgaa gaaatttcga gcgcattcct ttgtggtgat
      721 gccaaaccat tttcacttga tcttgaccgt ggcggagggt ctaaccctcg agcgagccat
      781 gcaactcatc aagggaggtt tctcccgcga agcaggcaga aggctcgggg cacgctatga
      841 gatctggcag aagagctttg tggatcgacg agtgcgcgac gctgttgaac ttgccagata
      901 tacagcctac attcaggaga atccagtgcg ggctggcttg gtcggcgttg cgggtgagta
      961 tcgctattcg tcgctgaatc ccgccttcat aatggatgaa ttacctcagc ggctgaagcc
     1021 gattttcaca gacgcacggt taatgcaccg ctaaagcggt actccaccct cggcttgtgg
     1081 tcgcgcgtcc tgatacaagg tcagcttcac gaacgcaaag cacgcttaag taccaccgcc
     1141 ttaaaggcgg tgggtttaac cctggcacgt cgaactaaac tcaccttcgg tcacgctggc
     1201 catccctttc atttacaatc caatcgacta tcattcccct cggagccttc atcacgctta
     1261 tgccaagccg gcctcaggtg ctcgtttctg ccttgctctt ctctgccctc gctatagccc
     1321 agactgcaac acccgccgcg aagtcttcgc aagcaccgcc cccagcaaaa tcgggttcca
     1381 aagcgctcac gatttcaccg aacggcgccg cgacgggctg gcgggccata cacaattcgg
     1441 cgctcgtcgt tgacacccac gccgacacgc ccggccgctt tgtggacgag aatttcgatc
     1501 tctcgcaaga cgccggcacc ggctatctgg acttcaacaa gatcaaggcc gggaatctcg
     1561 gggctgagtt tttctccatc tgggtggaac cgaaagccaa caaaggccac gagaccaagc
     1621 gcgcgctcga catgattgac tcggtttacg agcaggcgcg actgcatccc gacaagatga
     1681 tgatggcctt cagcacacag gacattctcg ccgcccatcg ccagcacaaa ctggcagcac
     1741 tgttgggggt tgaaggcgga cacgcaatcc aaggcgacat ccgcatcctg cgcgactact
     1801 accggctagg tgtgcgctat atgacgctga catggtcgaa caccaacgag cttggcgatt
     1861 ccagcggcga cctcaacgac aagagtgtcg agcaccacaa cggcatcacc gacttcggac
     1921 ggcaggtggt gcgcgagatg aaccggctcg gcatgatggt ggacatttcg cacgtggctg
     1981 atcgcacctt tttccaggcg ctggtggcca gtcgcgcgcc tgtcatcgca tcgcactctt
     2041 cgtcgcgcga gctcaccaac gctccgcgca acatgaccga cgatatgctg atcgcgctgg
     2101 cccgcaatgg aggcgtggcg caggttaatt tctattgcgg ttttatctct cagaagtacg
     2161 tggatcgcga aaagcagctt aaggaacagc atgatccgga ttacgagaag gtgcagtcgc
     2221 tcttcctcaa agcgcaaact cctgaggtca agaaagagtt ggatgaagct aaggcccggc
     2281 tcgtggtcaa gttaccgcgt ccgccgctga ccgatctcat cgaccatatc gatcacatgg
     2341 tgaaggtggc aggcgtggac cacgtcggtc tgggctccga ttttgacggt atcgattgtt
     2401 cgccgcaggg catcgactcc gccgccgacc tgcccaagat cacgcaggcg ctgtacgacc
     2461 gcggctacaa agccgcagac cttgataaga ttctcggcgg gaaccttatg cgagtattcg
     2521 ccgaagtgga gaagcttgcc aagcaaattc aggcagaaac caatcaggac acgcgccgcg
     2581 agattgtgcc cacggcgcaa tcgcaacagc agtaatgaag aactttactt cgggagaact
     2641 tatgcgaa
//
LOCUS       DASRKK010000057         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_882786, whole genome shotgun sequence.
ACCESSION   DASRKK010000057 DASRKK010000000
VERSION     DASRKK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882786"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..593
                     /locus_tag="VFC15_01135"
     CDS             249..593
                     /locus_tag="VFC15_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08795.1"
                     /translation="MPDAIATDPPQLPKHKLKYKKPLQRACNQPNEKGKLCGGHLKRW
                     FYLADVLEQACGDVRQTFGDQMEIYRCEHCKTLYLPGPEEPRGRNVAGVGSQSIFGLT
                     LTAKPGEEKEKQ"
     gene            complement(640..837)
                     /locus_tag="VFC15_01140"
     CDS             complement(640..837)
                     /locus_tag="VFC15_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08796.1"
                     /translation="MEAVIKQLNGLSFSMFVSLVFADKDLNLLGKQAANRGVPPGGKY
                     FGLAESRFTQTDRDILSMGIS"
     gene            916..>2014
                     /locus_tag="VFC15_01145"
     CDS             916..>2014
                     /locus_tag="VFC15_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM08797.1"
                     /translation="MGLGPPVRASSTEALSFSESQGSRSAQQQVARVAELRKVHEAFA
                     WFRSHARELEDLQLEVTSIAAPPWGEAARSAWLEARFNELALSDVHQDELGNVFGIRS
                     GTDPNAPYIALSAHIDTVFPAGTAIEVRRDAGKLYGPGVSDNSSGTVALLAIAGAMQA
                     ANLSNAAPVLFIGDVGEEGEGDLRGMRHIFQQPKWSATIASLVVLDGAGTDTIIAEGL
                     GSRRYEVTVRGHGGHSWSDFGVANPIVALARIIDRFARTPAPTLPKTTYNIGMINGGT
                     SVNSIPESATMKVDTRSASVEQLDRLENALHEAVDAVIPELTGRKKQELLTAEVRMIG
                     NRPAADLPVDSQLLKAIRAVDAQLNNASRIQR"
BASE COUNT          435 a          602 c          580 g          397 t
ORIGIN      
        1 gccgtgaggg atctgctgtt tcctcagcgg tcgttccagc gctaacaaaa gcagattcct
       61 cgcttcgctc gtaatgacaa ctccagagag gaatcgtcca taccacatgg gacattactc
      121 acgcaccgat ctatagccga cggtacagca ttgcgtagtg ttctgaaggc ccgcactacc
      181 caagctacag ccactccata acttcgctcg gtattgagat attcatcaca accagcgata
      241 ctgccttcat gcccgacgcg atcgcgactg atcctcccca gcttcccaag cacaagctca
      301 aatacaagaa gcccctgcaa cgcgcctgca accagcccaa tgagaaaggt aaactctgcg
      361 gcggacattt gaagcgctgg ttctacctgg ctgacgtgct ggagcaagcc tgcggcgacg
      421 tccggcaaac ctttggcgat caaatggaga tctatcgttg tgagcattgc aagacgctgt
      481 acctgccggg tccagaagag ccgcgtggcc gcaatgtcgc tggtgtgggg agtcagtcca
      541 tcttcggatt aacgcttacc gccaagccgg gtgaggaaaa agagaagcag taacttccgt
      601 ttgattgtag ctcaaatata tttcgccaga ggtgcgatgt cacgagattc ccatagacag
      661 aatgtcacga tcagtttgag taaaccgact ctccgcaagg ccaaagtact tgccgcccgg
      721 agggacccct cgattagcgg cctgcttgcc cagcagattg agatccttgt cggcgaagac
      781 gaggcttacg aacatgctga acgacaggcc attcaactgc ttgatcacgg cttccatctg
      841 ggaggactag cccccgccag ccgcgacgaa ctgcatgaac gataagacgt ttgttgatag
      901 tatggggcat cctccatggg tctcggtcct ccagtgcgtg cgtcttccac ggaagcgttg
      961 tccttcagcg aatctcaggg atcgcgttcg gcccagcaac aggtggcgcg agttgccgaa
     1021 ttgcgcaagg tccatgaggc gtttgcctgg ttccgttcgc acgctcgcga gctggaagac
     1081 ttgcagctcg aagtgacctc tatcgccgcg cctccatggg gcgaggctgc acgcagcgca
     1141 tggctcgaag cgcgcttcaa cgagttggcc ctttccgacg tgcaccagga tgagttaggc
     1201 aacgtcttcg ggatccggtc gggcaccgat cccaacgctc cgtacatcgc gctgagcgcg
     1261 catatcgata cggtgtttcc cgcgggaacg gcgatcgaag tgcgccgcga tgccggcaag
     1321 ctctatgggc ccggcgtctc cgacaactcg tcgggcaccg ttgccctgct ggcgattgcg
     1381 ggtgctatgc aggctgcgaa cctatcgaat gccgctccgg tcctctttat cggcgacgtg
     1441 ggagaagaag gcgaaggcga tctgcgcggc atgcgtcaca tctttcagca gccaaagtgg
     1501 tcggcaacca tagcttcgct ggtggtgctc gatggcgcag gaacggacac cattattgcg
     1561 gaaggacttg gcagccggcg ttatgaagtc acggtacgcg gccacggcgg ccattcctgg
     1621 agcgacttcg gcgtggctaa tccgatcgtc gcgctggcac ggatcatcga tcgcttcgcc
     1681 cgaacgcccg cacccacgct tccgaagacc acctataaca tcggcatgat caacgggggg
     1741 acgtcggtca actccattcc tgagtcggcg accatgaagg tggacacccg atcggcgtcc
     1801 gtggaacagc tcgaccggtt agagaacgcc ctgcacgaag cggttgacgc tgtcattccc
     1861 gaacttaccg gccgcaagaa acaagagctg ctgacggcag aggtccgcat gatcggcaat
     1921 cgtccggctg ccgatctgcc ggtggattcg caactgctga aagccatccg cgcggtggat
     1981 gcgcagctca acaacgcatc acgcattcag cggg
//
LOCUS       DASRKK010000058         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1176111, whole genome shotgun sequence.
ACCESSION   DASRKK010000058 DASRKK010000000
VERSION     DASRKK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176111"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1453)
                     /locus_tag="VFC15_01150"
     CDS             complement(260..1453)
                     /locus_tag="VFC15_01150"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HZM08798.1"
                     /translation="MSRPLLEMADIVRCAGQTFVERSRHWITWQHQKVLLAIARCRTA
                     VLGGHRDQCSHCGHSVISYNSCRNRHCPKCQGNARHRWLEARQRELLPTPYVHVVFTL
                     PRELAPLALQNKKLIYDLLFRTSAATLLEVARDPRHLGAEIGFFSVLHTWDQRLQLHP
                     PVHCVVPAGGLSLDHTHWIHIRYSFFLPIKVLSRVFRGKFVAALKRAGSEGKLRFYGD
                     LNQLAQPKMFSAWLRQLFRHDWVVYCKPPFGGPEHALRYLGAYTHRVAISNHRLVSLA
                     DGQVTFRWRDSAHKNKKRLMTLPVEEFLRRFLLHLLPRGFVRIRNFGFLANRRRALLL
                     PLCARLLDSVQESNLVNPPGPRPVRVPAFRICPRCGGPMLVIERLSAAQIQLRAPPLP
                     REHAA"
     gene            complement(1627..2346)
                     /locus_tag="VFC15_01155"
     CDS             complement(1627..2346)
                     /locus_tag="VFC15_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM08799.1"
                     /translation="MTRLRQIMLEELQRRNYAQTTIDCYIRTVEHFSRYFHRSPAQLG
                     PQHIREYQAALFKKWKLAPNTVNQRLAALRFFYIQTLKRAWSVAETPYPKKVLKLPII
                     LSQEEVARLINAALTPFHRIVLMTLYATGVRRAELARLKISDIDSQRMVIHIQGGKGR
                     QDRDVMLSPKLLDALREYWRGLKRKPSQWLFRGDSAIQRTVRSPPRPFITPAARPLDA
                     LACRRKTSIPTPCGIVSPPTC"
BASE COUNT          531 a          622 c          827 g          470 t
ORIGIN      
        1 gggcgctttc gatacagcga cttgaaggaa gcccctggct atgggatttg aatggtgctg
       61 aggtgggatg agcggggtgg caagccagtg ctggagtgtg aagggctggt tggatgagag
      121 atggaatcgg gttttgtgcg agcagcgtga tgggagtgac aggcggcgag tgagaaagcg
      181 actgggaaag cgacctgcga ggcgcaaaaa gcggtggcgt gccgtagcac gaggatggat
      241 cgaggttgga gctgtggttt catgcagcat gctcgcgggg aagcggcgga gcgcggagct
      301 ggatctgggc tgcagaaagc ctctcgatca caagcattgg tccaccgcac ctggggcaga
      361 tgcggaaagc aggaacgcga accgggcgcg gacctggcgg attgacgagg ttcgattctt
      421 gcacggagtc cagtaagcga gcacagagtg ggagcaatag agcgcgccgg cggttggcca
      481 gaaagccgaa gttgcgaatg cgaacgaagc ctcggggaag caggtggagc aggaagcgtc
      541 gcaggaactc ctcaacggga agcgtcatca ggcgcttttt gtttttgtga gccgaatccc
      601 tccagcgaaa cgtgacttgg ccatcggcca aggagacgag tcggtgattg gagatggcga
      661 cgcggtgggt gtaagcgccg agataacgca gggcatgctc gggaccaccg aagggcggtt
      721 tgcaatagac gacccagtca tgccggaaca gttgacggag ccaagcggag aacatcttgg
      781 gctgagcgag ctgattcaga tcgccataga agcggagttt tccttcgctg ccggcgcgct
      841 tcaacgcagc cacgaacttg ccgcgaaaaa cccgactcag cactttgatg ggaagaaaga
      901 aagagtagcg gatgtggatc cagtgggtgt gatccagcga gagtccaccg gcggggacca
      961 cgcaatggac agggggatga agttggagcc tctgatccca ggtgtgcagc acgctgaaga
     1021 aaccgatctc ggcgcccaga tgtctgggat cgcgagccac ttcgagcagg gttgcggcgc
     1081 tggtgcgaaa cagcaggtcg tagatgagct tcttgttctg cagggcgagt ggtgccagtt
     1141 cccgcggcag agtgaaaacg acatgcacgt agggagtggg tagcagctca cgctgacgtg
     1201 cctccagcca gcggtggcgg gcattgccct ggcattttgg gcaatgtcga ttgcgacacg
     1261 agttgtacga gatgacggaa tgtccgcagt gggaacactg atcacgatgt ccgccgagga
     1321 cagcggtgcg gcagcgtgcg atggccagca acaccttctg atgttgccag gtgatccagt
     1381 ggcgactgcg ctcaacgaat gtctgccccg cacagcgaac gatatcggcc atctccaaga
     1441 gagggcgact catttagggc tttggcgacg gctctcgttt ctgcttctcc ggggacaaaa
     1501 tcaaggagtc caacgggctc gcggtggcgc tgagatggcg tttggacaga tgcaggtaga
     1561 tggtggtttc ttccaagtcg tgatgcccca gcagcaactg aatggttcgc agatccgcac
     1621 cgtcttctaa caggtgggtg gcgaaacaat gccgcaaggt gtggggatgg atgtttttct
     1681 cctgcaggcc agcgcgtcga gcggcctggc ggcaggcgtg ataaacggtc ttgggggtga
     1741 tcggacggtc cgctgtatgg ctgagtcccc ccggaacaac cactgggaag gcttgcgctt
     1801 taaccctcgc caatactcgc gcaaggcgtc cagcagcttt ggactgagca tgacgtcacg
     1861 atcctggcgt cccttgcccc cttggatgtg gatgaccatc cgttgactat caatatcgct
     1921 gatcttcagg cgcgccagtt cggcacgccg cacgccggtg gcatagagcg tcatcagtac
     1981 gatgcgatga aagggagtca gtgccgcgtt gatcaggcga gcgacctcct cctgactgag
     2041 aatgatgggc aacttcagca ctttcttggg atagggagtt tcggccacgc tccaggcccg
     2101 cttgagggtc tggatgtaga agaaacgcag agccgccaac cgttggttca ccgtattcgg
     2161 tgccaacttc cacttcttaa acaacgcggc ttgatactcg cggatgtgtt gtgggcccaa
     2221 ctgtgcgggc gaacggtgga agtaccggga gaagtgttcg acggtgcgaa tgtaacaatc
     2281 gatggtggtc tgagcgtagt tacgacgctg gagttcttcc agcatgatct gacgtagacg
     2341 ggtcacagag aaaccctcct tcctctgcga gccaccttag atcaactccc tccaaactgc
     2401 tcgcccacga cacgcgcaag cgtccgccgt accacggctt cgttcaagtc
//
LOCUS       DASRKK010000059         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_491993, whole genome shotgun sequence.
ACCESSION   DASRKK010000059 DASRKK010000000
VERSION     DASRKK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491993"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFC15_01160"
     CDS             <1..366
                     /locus_tag="VFC15_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM08800.1"
                     /translation="VERAMILEDSDELHPEYMPFAVAQQQSAYTAFELSSANNSSNVA
                     GKRLPDGRSLPKLEIPEGGTSLEEVERELVDMAMAQANGNQTHAARLLDISRDALRYK
                     LKKFGLIHAEEDEPTTAAS"
     gene            697..1182
                     /locus_tag="VFC15_01165"
     CDS             697..1182
                     /locus_tag="VFC15_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08801.1"
                     /translation="MLLASQETRTFFASSATWERRLILQSNPLCDLLLDVIRENRAKQ
                     RFQVHEFVLMPNHVHLILTPAPLVSLEKAMQFIKGGFSYRAKKEMNFNCEIWQKGYNE
                     HRIRDADEYAQHVEYVWMNPVKAGLVEQPEEYLYSSARLKAEVDPAPFQFQLRARGTA
                     I"
     gene            1446..>2061
                     /locus_tag="VFC15_01170"
     CDS             1446..>2061
                     /locus_tag="VFC15_01170"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZM08802.1"
                     /translation="MRKLAIVIGVIVVIVVALVLALPHLIDVNRSRGQVQTQLQQRLN
                     RPVQLGQMSLSVLPLRVEVNNVTIGEDPSYRSNVPFAQVGQLNIGVKLFPLLAGNVEV
                     DSLEMKQAKIELIRNAQGVWNFSTAGNGPAATPSATQSAHDATAQQNQFTLGALKIAD
                     GEIAITDYQKHQTRAVYDHIDVTLKDYAPGSPFSIDATAHLPGSG"
BASE COUNT          473 a          608 c          555 g          425 t
ORIGIN      
        1 gtggaacgcg ccatgatcct ggaagacagc gacgaactgc atcctgaata catgccgttc
       61 gccgtggccc agcagcagtc cgcttatacc gcctttgagc tctcctcggc caacaacagc
      121 agcaacgttg ccggcaagcg gttaccggat ggccgcagcc tgcccaagct ggagattccc
      181 gagggtggca cttcgctgga agaggtggaa cgcgagttgg tggacatggc catggcgcag
      241 gcgaatggca accaaaccca tgctgcacgc ctgctcgaca tcagccgcga cgccctgcgt
      301 tacaagttga agaagttcgg cctcatccat gccgaagaag acgagcccac caccgccgcg
      361 tcgtagcgtt caattgaaca ctgagtgacc ctgagcccgc agggcgacat aatcctagcc
      421 cagcacgtga gtgcaatgag ccttaacgta acctgtgtaa ctgagaccag taaagttgtt
      481 cggtgggagc ccccgcattc atgcggggga aggagcgctt cagcgctccg ggaaagagtt
      541 cgactttgat catgcgcttt agcgctggca tcgagaaatc ccgggcttac agtcgcccga
      601 ttgattcaag caccgctctt tcccccgcat aaacgcggag gctcccatcc agaagaagcg
      661 ttccctcacc tgatgatata aacttcggct tccactatgt tgcttgcctc gcaagaaact
      721 cgtacttttt tcgcctcgtc tgcgacttgg gagagaagat tgatcctgca gtccaatcct
      781 ctttgcgatc tgctgctgga cgtaattcgt gaaaatcggg ccaaacagag atttcaggtg
      841 cacgagttcg tcttgatgcc aaatcacgtt catctgattc tcacgcccgc tccgctagta
      901 tcgcttgaga aagcaatgca attcatcaag ggaggattct cttaccgcgc gaagaaggag
      961 atgaacttca attgtgaaat atggcagaag gggtacaacg agcacaggat cagagacgca
     1021 gatgagtatg cgcaacacgt tgaatatgtc tggatgaatc ccgtcaaagc aggtttggtg
     1081 gagcagcctg aggagtatct gtactcctcg gcgcgattga aggcggaagt cgatccggcg
     1141 ccgtttcaat ttcaactaag ggctcgcggt acggccatct aaatcccggg gctaaagccc
     1201 gattgattga agcacagctc tttctcgcaa cgctaaagcg ttgcttcccc cgcatgaatg
     1261 cgggggctcc caccgaacaa ctttgctggc ctcagttaca cgaattaggt taacgctcac
     1321 gtcctgggct acgattgttt cgcccgtcgg gcttttgatt acaccagcgc atccgccttc
     1381 tatctacacc cagcgaattt cactacaatt aatccagccc cgccacttcg gaccgaggag
     1441 gacccatgcg aaagcttgca atcgtaatcg gagtcatcgt cgttatcgta gttgcgctgg
     1501 tcttggcgct gcctcacctg atcgacgtca ataggtcccg cggccaagtc caaacccagt
     1561 tgcagcagcg actcaaccgt ccggtgcaac tgggacagat gtcgcttagt gtgctccctc
     1621 tccgggtgga agtgaataac gtcaccatcg gggaagatcc cagttaccgc agcaacgtgc
     1681 ctttcgccca agtcggccaa ctcaacatcg gcgtgaaact attcccgctg ctcgccggca
     1741 acgttgaggt agacagtctg gagatgaagc aggcgaagat cgaattgatc cgtaacgcgc
     1801 agggcgtgtg gaatttctcg acggctggga acggcccggc ggccactcct tccgccacgc
     1861 agtcggccca tgacgccacc gcccagcaga accagttcac gctcggcgca ttgaagattg
     1921 ccgacggcga aatcgcgatc accgactatc agaagcacca gacgcgcgcc gtttacgacc
     1981 acatcgacgt gacgctgaag gattacgctc ccggctcgcc gttctccatt gacgccacgg
     2041 cccacctgcc gggcagtggt g
//
LOCUS       DASRKK010000060         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_296448, whole genome shotgun sequence.
ACCESSION   DASRKK010000060 DASRKK010000000
VERSION     DASRKK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296448"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1066)
                     /locus_tag="VFC15_01175"
     CDS             complement(350..1066)
                     /locus_tag="VFC15_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08803.1"
                     /translation="MDEKRYSCQHIVPIEQEPRHHLVIENEFVRAFVVEIAPHDRTLC
                     HHHPHDYLLYVAGDGDIVSAARDEEPKKLSYRDGECELLQAGMVHVVENLGDTAFRNV
                     VVELLPGTGSLRRGDEPKLISGEASITEHFADEHAAIFMIAMDAGSEVQVHGRLVVAS
                     PYEHEVELEDFRGDAVKLSEFNYIAWLWSPLKATLRNIGDSPAKAVLFQIGRADEELL
                     LVQKQVEPTKSLRTHADETE"
     gene            complement(1069..1818)
                     /locus_tag="VFC15_01180"
     CDS             complement(1069..1818)
                     /locus_tag="VFC15_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08804.1"
                     /translation="MKIHALLWMLCLTAGLALAQDTKQITPNKFGPTVEIPADATHHP
                     KIENEYFRAYYVEVAPQQSTLMHHHGNDYVSVALGHTVIDSIAPDGAVKHVVLEDGDV
                     RYTPAGLIHAVTNVASTPFHNATIELLQNHGHPVCVNNCASDPRAKDWPPLTEESKLI
                     GYGDSFRISEAVIKPQQTVSNEEAYPHLVILLTDMHAHTGPAGSGGMDFNQKAGDMMF
                     HGGHPDHGFTNVGGQDMRLIVMEFKPAPTKD"
     gene            1937..2013
                     /locus_tag="VFC15_01185"
     tRNA            1937..2013
                     /locus_tag="VFC15_01185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1971..1973,aa:Pro,seq:ggg)
BASE COUNT          408 a          608 c          568 g          446 t
ORIGIN      
        1 tgccggagaa ctctgtctcc gacttccagc cctgtggcca gggcagctta ctacgaagct
       61 ttatgtctta gatgccggct tctcctgcag gactcaaaat cagtcccgca tccgcaggcg
      121 acagtaatgc ctctcgctga catatttgtt atagcagacg aggacggatg acacaacccc
      181 cgagctgaaa ttattgccaa agatcttggt tcggatcgat gaaagcctca taaatactgg
      241 cgatgcgcgc gagaaaaaaa tttctccgaa acgatgattt ttctctccag gtcagggggg
      301 cagcgccaga ttccacatga ttcccacacc ctttacacat cgggcaggtt cattcagtct
      361 cgtcggcgtg agtgcgcaga ctctttgtcg gttcgacttg tttctgtacc agcagcaact
      421 cctcatcggc acggccaatc tggaacagaa ccgcctttgc aggactatcg ccgatgttcc
      481 tgagcgtcgc cttcaagggc gaccacagcc atgctatata gttgaattca gacagcttaa
      541 ctgcgtctcc cctaaagtct tccagctcca cctcgtgctc gtacggagac gccacaacaa
      601 gccggccgtg gacttgcacc tccgagccag catccatcgc gatcatgaat attgccgcat
      661 gctcatccgc gaagtgttcg gttatgctcg cttctcctga aatcagcttc ggctcgtcgc
      721 cgcgccgcag actgcccgtc ccaggcagta actccacgac aacattgcgg aacgccgtgt
      781 cgcccaggtt ctcgaccaca tgcaccattc ccgcctgcaa cagctcgcac tctccatcgc
      841 ggtaactgag cttcttcggc tcctcgtcgc gcgctgcgct aacgatgtct ccatcgcccg
      901 ccacgtagag cagataatcg tgcggatgat gatggcaaag cgtgcgatcg tggggcgcga
      961 tctcgactac aaacgcgcgc acaaactcgt tctcgatcac gagatgatgc cgcggctcct
     1021 gctcgatggg aacgatgtgc tggcacgagt atcgcttctc gtccacagct agtctttcgt
     1081 gggcgccggc ttgaactcca tcacgatcag gcgcatgtcc tgaccgccaa cattggtgaa
     1141 cccatgatcg gggtggccgc cgtgaaacat catgtcgccc gccttctggt tgaagtccat
     1201 gccgccgctg ccagcgggtc cggtgtgcgc gtgcatgtca gtcagcagga tgaccagatg
     1261 gggataagcc tcctcgttgg acaccgtttg ttgcggcttg atgaccgcct ccgagatgcg
     1321 gaagctgtcg ccatagccaa tcaacttcga ttcttccgtc agtggaggcc agtccttggc
     1381 gcggggatcg ctggcacaat tgttcacaca caccggatga ccatggttct gtagcagctc
     1441 gatggtcgcg ttgtggaacg gtgtgctggc cacgttggtg acagcatgga tcagccccgc
     1501 cggcgtgtaa cgcacatcgc catcttccag cacgacgtgc ttgaccgcgc cgtcgggcgc
     1561 gatgctgtcg atcacggtgt gcccgagcgc gacggaaaca tagtcgttgc cgtggtggtg
     1621 catcagggtt gactgttgcg gcgctacctc aacgtagtaa gcgcgaaagt attcgttctc
     1681 gatcttcgga tggtgagtgg cgtccgcggg gatctcaacc gtggggccga acttgttcgg
     1741 ggtgatttgc ttcgtgtcct gagccagcgc gaggccggct gtcaggcaca gcatccatag
     1801 cagtgcatga atcttcatgc cggacagagt agcgatgcgg cagcagcatt acaagggaga
     1861 agttggctgc gcggtggtac actatcggtg acctcgaaac ccttccggtt cgcatggacc
     1921 ggactcacag accagtcggg acgtggctca gcctggtaga gcactcgctt ggggtgcgag
     1981 gggtcggcag ttcaaatctg cccgtcccga ccaaaatcag cactcagctt
//
LOCUS       DASRKK010000061         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_589665, whole genome shotgun sequence.
ACCESSION   DASRKK010000061 DASRKK010000000
VERSION     DASRKK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589665"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="ccsA"
                     /locus_tag="VFC15_01190"
     CDS             <1..462
                     /gene="ccsA"
                     /locus_tag="VFC15_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZM08805.1"
                     /translation="DAAYPSPDQDGLFPTSLAASPFLLGGMVTGIFASLLYLMLLWRR
                     SDLQGLLPSADTLDRVTYKTICIAFPLLTGMIAAGAYWANRTWGSYWSWDPKETWAAI
                     TWLVYAGYLHMRITRGWRGRRAAYFAILGFGVVIFTFLGVTYLLPGLHAYA"
     gene            573..1721
                     /locus_tag="VFC15_01195"
     CDS             573..1721
                     /locus_tag="VFC15_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM08806.1"
                     /translation="MSASSPFRLYDADPPARAETPPWEQPPVARKRHLNWALKAVLPI
                     AAVLLNGLLLFVLVSLSLAEGERHVILAVAAAGALLICVAVIMVLAVSVRRPMLELQD
                     KIARVRLGDMDVAVSFASRNDEIGDLGRDFNDMVAQLEASREEIQRLHQTQMSRAEHF
                     ATLGELAAGLAHEIRNPLAGIAGVIEIIGRDLPPNSPARSVIKDAKEEAVQINRILTE
                     LLEIARPKPPQFRVGDMCSTVEHAVMFARQQAITKRIVVELEIKDAIPPAEHDPNQIN
                     QVLLNLLLNAIQSMDKPGVIRVSLESDEDDVLITVADEGKGISPEHLPNIFRPFFTTK
                     GHGTGLGLSLARRMVEAHGGHIDVTSVVGKGTEFQVRLPIARLQAATS"
     gene            1718..2413
                     /locus_tag="VFC15_01200"
     CDS             1718..2413
                     /locus_tag="VFC15_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08807.1"
                     /translation="MSSTESKPVISKWRFWAPLIASAIFIPVLLLGIVEVALRFSGVG
                     FPTELLVPCTLQGRPASCYNLFFPAPFFPPGMIKTPQVYAIPAEKPQGTFRIFVLGES
                     AAMGDPDPAYAFSRYLEVMLRERFPSIRFEVVNTGSVAVNSHVLVPIARDLAKQRPDV
                     FIIYSGNNEVVGPYGPGTALTASGMNLTVIRSSIFLRSTRIGQLVTKLGTPKKSGAAW
                     RCSSTSNCQPLRP"
     gene            2374..3381
                     /locus_tag="VFC15_01205"
     CDS             2374..3381
                     /locus_tag="VFC15_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08808.1"
                     /translation="MFLDKQLSATSPLMAPTYENFASNLRDTVEAARSSGARVLISTV
                     ASNLKDCGPFASMHHAGLSQDALASWSQLVQQGANLENARSYADALQEYRSAAKIDDQ
                     FAELEFRIARVLGKMGANQEAKEHYLRARDLDTLRFRADSKINEINRSTASSISGAEL
                     VDAEAILSQESPDGIIGNDLLYDHVHLTPRGNYLLARAAFLKIASQLPAESGYALQAD
                     VPSQAECERLLALTNHDRTRMATEMLQRLQRPPFTNQLDHADQLLRLMIDADATHESP
                     DETVAQYQWALQQSPDDRILHYNFGLFLFDYNSEASTQELRMSRPWDGFPVFAPNGTL
                     L"
     gene            complement(3446..4231)
                     /locus_tag="VFC15_01210"
     CDS             complement(3446..4231)
                     /locus_tag="VFC15_01210"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZM08809.1"
                     /translation="MTFGFGLQLFFDFAGYSHIAIGAAKVLGFTVPENFARPFQSTSP
                     SIFWTRWHMSLSFWIRDYVFLPLAVLRREMWWRSLVLVLSMVLFGLWHNASLLFIIWG
                     CYHGVLLVLHRQAQQLQRRFDWHPPAFLWAPVSWSATFILISLGWIFFRAHSLPKAGQ
                     MLSAVLSPASYLSHVVSGSLYLLVMALGCGYVIVLLGIEALDRYSVGTGVSHQHVGSG
                     VISFLARKRWFWIPPLYAVTLLLVLAVTLNQGPGMSQLMYRAF"
     gene            complement(4228..4884)
                     /locus_tag="VFC15_01215"
     CDS             complement(4228..4884)
                     /locus_tag="VFC15_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08810.1"
                     /translation="MYVLIFLIELAIFAKFRSVKVRQACLLAGSCALYLSWGIWFGAV
                     LLASIVMNYLLGAWIRRTPSALILWTGILLNLLVLTTFKYLPGVAIPLPFSSLQKLAH
                     LALPLGISFWTFQAMSYLFDLYRGEELQPSFVEFALYMVFFPVTISGPICRVPDMLPQ
                     FRSEKAIDWSSVERGFGRIATGVLMMQLARLLGQGILAGNGIDGFDRMAHWSELTFGV
                     "
     gene            4993..5157
                     /locus_tag="VFC15_01220"
     CDS             4993..5157
                     /locus_tag="VFC15_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM08811.1"
                     /translation="MPPKHGTGLGLSLARRMVEAHGGHIDVTSVVGKGTEFRVRLPIK
                     SQTDQVHIVS"
     gene            complement(5177..6676)
                     /locus_tag="VFC15_01225"
     CDS             complement(5177..6676)
                     /locus_tag="VFC15_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM08812.1"
                     /translation="MQAAQYARMSTEHQQYSIQNQEAAIALYAQEHGFDIVRSYRDEA
                     KSGLDLAHRPGLRALLEDVVNPQRGFVAILVLDVSRWGRFQDIDESAYYEYTCKRQGV
                     RVHYCAEPFANDESLTTVLLKTLKRAMAGEYIRELSTKVFVGQCRIARNGYKLGGTPG
                     YGLRRLLVDREGHAKCILHEGEWKSLSSDRVTYVPGPIEEVRVVQEIYSRFVSDNWSA
                     SKIAGELNMRGIRRDTGAEWDYFSVYDILRNAKYSGSIVFNRTSTKFKGKTRANPRDE
                     WIVQPNSFEALVPPEMFDQAQKRFSPNGRREKLLDDLRRLLQSRKRLTGKIIEGDPQI
                     ASPAAYHRNFGSLKNAYKLIGYERSTNVERVAKDQMRRSHFRMVKLRIMTALLREFVR
                     RSIPVEPYSQGVVLVGYGRLEIQIARSLNPAFKGPRWDIVCAHPQSRQFMVIRMHVDN
                     IRVRDYLLFHCLPAPRHSRLFIYESEISDFAISFKDIPALIAEIVPIDR"
     gene            complement(6867..>7112)
                     /locus_tag="VFC15_01230"
     CDS             complement(6867..>7112)
                     /locus_tag="VFC15_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08813.1"
                     /translation="NGVAERWVGSCRRDLLDHVIALDERHLKRLVSQYISYYHDDRTH
                     LGLEKQTPAGRARSLENGTVICQQRVGGLHHRYDWAA"
BASE COUNT         1636 a         2097 c         1929 g         1450 t
ORIGIN      
        1 gatgcggctt acccgtcacc cgaccaggat ggactcttcc cgaccagtct ggctgccagc
       61 ccgttcctac tggggggcat ggtgacgggt atttttgctt ccctgttgta cctgatgctg
      121 ctgtggcggc gcagcgacct ccagggtctg ctgccctcgg ccgacacgct cgaccgcgtc
      181 acctacaaga ccatctgcat cgccttcccg ctgctgaccg gcatgattgc cgccggcgcc
      241 tactgggcga accgcacctg gggctcatat tggagttggg acccaaagga aacctgggcc
      301 gccatcacct ggctggtcta cgcgggttac ttacacatgc ggatcacgcg gggatggcgc
      361 ggacgtaggg ccgcgtactt cgccattctg ggcttcggcg tcgtgatctt cactttcctc
      421 ggcgtcacgt atttgcttcc gggcttacac gcgtacgcat gaggaacagg gctggccgat
      481 ccggttctga ggatggatta ggccccaaga ttcaggccga tcccccagcc ctgaaatttc
      541 aagtcgtcaa ggagacgagc agtagcttac agatgtcagc ctcatcccca ttccgactct
      601 atgatgccga tcccccggcg cgagctgaga ctccgccctg ggagcagccg ccggtggcgc
      661 gtaagcgcca tctcaactgg gcgctcaaag cggtgttgcc gatcgcggcg gtattactga
      721 acggcctgct gttgtttgtg ctggtttccc tgtcgctcgc cgaaggagaa cggcacgtta
      781 tcctcgccgt cgccgccgct ggcgcgttac tcatttgcgt cgcggtcatc atggtgctgg
      841 cagtttccgt ccgccgtccc atgttggagt tgcaggacaa gatcgcgcgg gtccggttgg
      901 gcgacatgga tgtcgccgtc agctttgcct cgcgcaacga cgagattggc gacctgggac
      961 gagacttcaa tgacatggtg gcgcaactgg aggccagccg cgaagagatc cagcgtctgc
     1021 atcagacgca aatgtcgcgc gccgaacatt tcgccactct gggagaactg gcagctggac
     1081 tggcccacga aatccgcaat cctttggccg gcatagccgg agtgatcgag atcataggca
     1141 gagatctgcc gcccaacagc ccggcgcgtt ccgtcatcaa ggacgccaag gaagaggcgg
     1201 tgcaaattaa tcgcattctc accgaactgc tggagattgc ccggcccaag ccgccacaat
     1261 tccgcgtcgg cgatatgtgc agcaccgtgg aacacgcggt gatgttcgcg cgccagcagg
     1321 ccatcaccaa acgcatcgtt gtcgaattgg aaatcaaaga tgcgattccg cccgccgaac
     1381 atgaccccaa ccagatcaac caggtgttgc tgaacctgtt gctcaacgcc attcagtcca
     1441 tggacaagcc cggtgtcatc cgcgtttcct tggagagcga tgaagacgac gtgctcatca
     1501 ctgtggctga cgaagggaaa gggatttcgc cggaacacct gcccaacatc tttcgtccct
     1561 tcttcaccac caaggggcac ggcaccggat tgggactctc gctcgcccgc cgcatggtgg
     1621 aggctcacgg cggccacatc gacgtcacca gcgtcgtagg caaaggaacg gaattccagg
     1681 ttcgactacc aatcgcacgc ctgcaggccg caacctcatg agtagcacgg agtccaagcc
     1741 ggtgatttca aaatggcgtt tttgggctcc gctcatagcg agcgccatct tcattccggt
     1801 attacttctt ggaattgtgg aagtggcatt gcggttttcg ggagtgggct tccccaccga
     1861 attgctggtc ccgtgcacgc ttcagggccg gcctgcttct tgttataacc tcttcttccc
     1921 tgcgccattc tttcctccgg gcatgatcaa gacgccgcag gtgtacgcga tccctgcgga
     1981 gaaaccgcag gggaccttca ggattttcgt actcggagaa tccgcagcta tgggagaccc
     2041 agatcccgcc tatgcattca gccggtacct ggaagtgatg ctccgcgaac gcttcccatc
     2101 cataaggttc gaggttgtca acaccggcag tgtggccgtc aattcacatg tgctcgtgcc
     2161 gatcgctcgc gacttggcaa agcaacgtcc ggatgtattc atcatctact cgggcaacaa
     2221 cgaagtggtc ggaccctatg gtcccggcac tgcactgaca gcctcgggca tgaacctgac
     2281 ggttatccgc tcgagtatct ttcttcgctc cacgcgcatt ggccagcttg tcaccaagtt
     2341 ggggaccccg aaaaaaagtg gggcggcatg gagatgttcc tcgacaagca actgtcagcc
     2401 acttcgcccc tgatggcgcc cacctacgaa aatttcgcca gcaacttgcg cgatactgtt
     2461 gaggccgctc gaagttcagg cgccagggtg ttgatcagca cggttgccag taacctgaaa
     2521 gactgtggcc cattcgcctc catgcaccac gccggcttga gtcaggatgc gttggcttcc
     2581 tggtcgcaac tggtccagca gggcgccaat ctggagaatg caagatccta cgcagacgca
     2641 ttacaagagt accgctcggc cgcgaagatt gatgaccaat tcgccgagct tgaatttcgg
     2701 attgcgcggg tcctcggcaa gatgggagct aaccaggagg ctaaagagca ctatcttcgg
     2761 gcccgcgatc tcgacacgct gcgattccgt gccgacagca agataaacga gatcaaccgc
     2821 tccaccgcgt cctcgatctc gggcgcagaa ttagtggacg ctgaagcgat tctttcccag
     2881 gagagtcccg acggaatcat cggtaacgac cttctctatg accatgtgca cctcacgccc
     2941 aggggaaact atctgttggc tcgtgccgcg tttctgaaga tcgcaagcca actgcccgca
     3001 gagtctggat acgccttgca agccgacgtg ccctctcagg ctgagtgcga gcggctgctg
     3061 gccctcacca atcacgatcg cactcgcatg gcgacggaga tgttgcagcg gttgcaaaga
     3121 ccacctttta ccaatcaact tgaccatgcc gatcagttgt tgcgattgat gatcgatgcg
     3181 gacgcgaccc acgaaagccc ggacgaaaca gtcgcccagt atcaatgggc gctccagcag
     3241 agcccggacg accggatcct gcactacaac ttcgggcttt tcctgttcga ctacaacagc
     3301 gaagcctcca cgcaggagct gcgcatgtcc cgcccttggg acggatttcc ggtgtttgct
     3361 ccgaacggca cactgctgtg aatagaagga tccaccctct ctgattcctg cttgggtttt
     3421 gtgaggctgg accagctcag caagcctaga aagcgcgata catcaactgg gacatccctg
     3481 gaccctggtt cagcgtcacg gctaacacca gcagcaaggt cactgcgtag aggggcggaa
     3541 tccaaaacca gcgcttgcgg gcaaggaaag agatgactcc cgaaccaacg tgctggtgcg
     3601 agaccccggt tccaacggaa tagcgatcga gagcctcaat cccgagcagt acgatgacgt
     3661 atccgcatcc cagggccata accagcagat acaaactccc actgaccacg tgcgacagat
     3721 agctcgccgg cgacaggacc gccgacagca tttggccggc cttgggaaga gaatgcgcgc
     3781 gaaagaagat ccatcccaaa ctgatgagaa tgaacgttgc gctccacgag acgggcgccc
     3841 acaaaaaggc aggcgggtgc cagtcaaacc tgcgctgcag ttgctgcgcc tgccgatgca
     3901 ggacaagcag tactccgtga taacagcccc aaattatgaa tagcaggctg gcattgtgcc
     3961 ataagccgaa cagcaccatc gaaagcacca gaaccagact tcgccaccac atctcccgcc
     4021 gcaacacggc cagcggcaag aagacatagt cgcgaatcca gaaggagagc gacatgtgcc
     4081 agcgcgtcca gaagatggat ggggatgtgg attgaaacgg tcgagcaaaa ttttcgggca
     4141 ctgtgaaacc cagaaccttg gccgcgccga tggcgatgtg cgagtatccg gcgaaatcga
     4201 agaacagctg taatccgaat ccgaacgtca aacaccaaac gtcagctcac tccaatgagc
     4261 catgcgatcg aagccgtcga ttccgttgcc ggccagaatt ccctgtccca gcagccgcgc
     4321 caactgcatc atcagcactc cggtagcaat cctcccgaat ccccgctcga cactgctcca
     4381 atctatcgcc ttctcagaac gaaactgtgg cagcatatca ggcacgcggc agatcggccc
     4441 tgaaatcgtc accgggaaga aaaccatata caaggcaaac tcgacgaatg aaggctgcag
     4501 ctcctcccca cggtaaaggt caaacaggta actcatggcc tggaaggtcc agaacgagat
     4561 acccaaaggc agggcaaggt gggcaagctt ctgcaacgac gagaaaggaa gagggatcgc
     4621 gactcccggt aaatatttaa aggtcgtcaa gactagcaaa ttcagcagga tgccggtcca
     4681 gaggatgagc gcagaagggg ttcgccgaat ccaggcgccc agaagatagt tcattacgat
     4741 ggaggcgaga agcacggccc cgaaccagat cccccaggag agatacaagg cgcaactgcc
     4801 cgccaggaga caggcctggc gcaccttgac tgatcgaaac ttggcgaaga tcgccaactc
     4861 aatcaggaaa ataagaacgt acaagatcgc cctggccaac attcccccca agttgggact
     4921 aagacaacta gtttcgcatt gattcgactg ccgtaaagct ctttccttgc ggactagctc
     4981 accggcagag atctgccgcc caagcatggc actggattgg gactttccct cgcccgccgc
     5041 atggtggaag cgcacggcgg ccacatcgac gtcaccagcg tggtaggcaa gggaaccgaa
     5101 ttccgggtcc gattgccaat caagagccaa accgaccaag tgcacattgt cagctgaaat
     5161 tagtaacgcc cgacgatcat ctgtcgatcg gaacgatttc agcaatgagt gccggtatat
     5221 ctttgaaact tatagcaaag tctgatatct cggactcgta aatgaagagc ctcgagtggc
     5281 gcggagcagg gagacagtga aacagcagat agtcacgcac gcggatattg tcgacatgca
     5341 tgcgtatgac catgaactgc ctcgattgag ggtgggcaca cacgatatcc caacgtggac
     5401 ccttgaatgc cggattgagg ctgcgcgcaa tctgtatctc caaccgtccg taacctacta
     5461 gtaccacccc ttgtgaatac ggctcgacag gtatggatct gcgcacaaat tcccgcagca
     5521 aggctgtcat aatacgcaat tttaccattc ggaagtgcga cctgcgcatc tgatctttgg
     5581 caacacgttc gacatttgtt gaacgctcgt acccgatcag cttatacgca ttctttaggc
     5641 tgccaaaatt gcgatggtag gctgctggag atgcaatttg tgggtcgccc tcaatgattt
     5701 tgcccgttag ccgcttccgc gattgaagta accggcgtag atcatcaaga agtttttctc
     5761 tacgaccgtt tgggctaaac ctcttttggg cttggtcaaa catttctggc gggaccagcg
     5821 cttcaaaact attgggctgt acaatccatt cgtctcttgg gtttgctcgg gtctttccct
     5881 taaacttggt gctagtccga ttgaatacga tgctgccaga gtatttcgcg tttctgagaa
     5941 tgtcgtaaac cgagaaataa tcccattcgg cgccggtatc acgtcgaatg ccacgcatat
     6001 tgagttcacc cgcaatctta gaagctgacc agttgtcgct cacaaagcgg gaataaatct
     6061 cctgtaccac gcgaacctct tctattggcc ctggaacata cgtcactcga tcagagctta
     6121 ggctcttcca ttcaccctca tgcaggatac attttgcgtg gccttcacga tcaacaagga
     6181 ggcgcctcaa gccgtaaccg ggggtgccgc ctagcttata gccattcctc gctatgcggc
     6241 actgcccgac gaataccttg gtggagagtt ctcgaatata ctcaccggcc atagcccgtt
     6301 tcaacgtttt tagtaagacc gtggtgaggc tttcgtcatt cgcaaacggc tcagcacaat
     6361 agtgcacccg aacaccctga cgcttacagg tgtattcgta ataagcactt tcatcaatat
     6421 cttggaaccg tccccaacgg ctgacgtcga gaacgaggat ggcgacaaat ccgcgttgag
     6481 gattgacgac atcttccagc agagcacgga gaccaggccg atgcgcgaga tctaagccgc
     6541 tcttggcttc gtcacggtag ctgcgcacaa tatcgaatcc gtgctcctgt gcataaagag
     6601 caatcgctgc ctcctgattc tggatggagt actgctggtg ttctgtggac attcttgcat
     6661 actgcgcagc ctgcataatg ggccctgtgc ctcgtcacaa tccaaaacgc accaggagca
     6721 gtgcatactc aagagttccc tgacagcagg atctaacttt tctgtggcgt tgaatggcca
     6781 aacgcgcggg acttcttcgt agctggctga atgagcggac acttctgtcg tgggtccaga
     6841 caaatgaaag ctcgcctaca gccgattcaa gcagcccagt catagcggtg atgcaacccg
     6901 cccacgcgct gctggcaaat cactgtaccg ttttccagag agcgtgccct accagccggc
     6961 gtttgcttct ccagcccgag gtgggttcgg tcgtcgtgat aataggaaat gtactgggag
     7021 acgagtcgct tcagatgccg ctcatccaac gcgatcacgt ggtcgagcaa gtcgcggcgg
     7081 cagcttccga cccaacgctc ggcgacccca tt
//
LOCUS       DASRKK010000062         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_980781, whole genome shotgun sequence.
ACCESSION   DASRKK010000062 DASRKK010000000
VERSION     DASRKK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980781"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFC15_01235"
     CDS             <1..1359
                     /locus_tag="VFC15_01235"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZM08814.1"
                     /translation="VKFHWRPKLGLQSTIWDETVKICGADQDFHRRDMFEAIAAGHFP
                     EWEFAVQLFTQEEADNFPFDHLDATKLIPEELVPLKVIGRMVLDRWPDNFFAETEQVA
                     YCPANVVPGIDFSNDPLLQGRLFSYVDTQLSRLGSVNFHQIPINAPKCPFGNQQRDGH
                     MQMEQPAGRVAYEPNSLSENSPRETPAKGFHSAAVTESGEKGRIRLERFADHYSQARQ
                     FYLSQSKYEQAHIASALVFELSKVEHAHVCKAIVGHLRHIEEDLAKRVAAGLGFDKMP
                     DAPMTAAPVQEMDPSPALQIIGKMKDTLMGRAIGILIADGSDGAVIKKIKKTATDAGA
                     TVKIVAPKVGGARLADGSMLAADGQLAGTPSVLFDAVAVILSDEGAKALSMESAAIDF
                     VRDAFGHLKAIVADKGGQALLRRANVGRDAGVVDANDIDTFIAAAKTRQWDRENSVRT
                     LA"
     gene            1650..>2257
                     /locus_tag="VFC15_01240"
     CDS             1650..>2257
                     /locus_tag="VFC15_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZM08815.1"
                     /translation="MTKAGPAQSSLANYRAKREFTKTSEPAGKKEGPAPSKQRFVIQR
                     HDATRLHYDFRLELDGVFKSWAVTRGPSLDPHDKRLAVEVEDHPLDYGDFEGTIRKGQ
                     YGGGTVQLWDRGYWIPEGDPHDGLKRGDLKFSLEGERLHGGWVLVRMKNDRKGAKRTN
                     WLLLKHRDDDARDGDGDALLRDPKSIASGRSLAAIAAGKGKAP"
BASE COUNT          473 a          651 c          711 g          422 t
ORIGIN      
        1 gtcaaattcc actggcgtcc caagctcggc ttgcagtcca ccatttggga tgagaccgtc
       61 aagatttgcg gcgctgatca agacttccac cgccgcgata tgtttgaggc catcgcggcc
      121 ggtcatttcc ccgagtggga attcgcggtt cagcttttca cgcaggagga agcggacaac
      181 ttcccgtttg atcatctgga cgcgacgaag ttaatacctg aagaactcgt gcccttgaag
      241 gtgatcggcc gcatggtgct ggaccgttgg ccggacaatt tctttgccga aaccgaacag
      301 gttgcatact gtcccgccaa tgttgtgccg ggcatcgatt tctcgaatga tccactgttg
      361 caaggccgtt tattctccta tgtcgacacg cagctttcgc gcctgggttc ggtcaatttc
      421 caccagatcc cgatcaacgc ccccaagtgc ccgttcggaa accaacaacg cgacggacac
      481 atgcagatgg agcaaccggc gggccgtgtc gcgtatgagc ccaactccct gtctgaaaat
      541 tcgccacggg agacgccggc caagggcttt catagcgctg cggtcactga aagcggcgag
      601 aaaggccgca tccgactcga gaggtttgcc gaccactaca gccaggcgcg gcagttctat
      661 ctcagccaat ccaagtatga gcaggcgcac atcgcctcgg cgctggtgtt tgagctttcg
      721 aaggtcgagc atgcgcacgt gtgcaaggcg attgtcggtc acctgcggca catcgaagaa
      781 gacctcgcca aacgggtcgc cgcgggtctt gggttcgaca agatgcccga tgcgccgatg
      841 accgcagcgc ccgtgcagga gatggatcca tcgccagcat tgcagataat cggcaagatg
      901 aaagacacgc tcatgggacg cgcgattggc atcctgatcg cggatggttc agacggagct
      961 gtcatcaaga agattaagaa gaccgcgacc gatgcgggtg ctaccgtgaa gatcgtcgcc
     1021 cccaaagtgg gcggcgcgag gcttgccgat ggctcgatgc tggccgctga cggacaactg
     1081 gctggtaccc cttccgtgct attcgacgca gtcgccgtca ttctctccga cgaaggcgca
     1141 aaggcactgt cgatggaaag cgccgcgatc gattttgtgc gggatgcttt cggtcatctc
     1201 aaggccatcg ttgccgacaa aggcggccaa gcgcttttga gaagagcgaa tgtcggacga
     1261 gacgcgggcg tcgtggatgc caacgatata gacaccttca ttgccgcggc aaagacacgc
     1321 caatgggacc gtgagaattc cgtgcggaca ttggcttaac ggccggttcc gtagaacgtg
     1381 aaggactaag gcatgtggat cggttccgta gttgcagcac gtgtcgacga cttgcgtgac
     1441 cgggctcagt ccttcggcga ggccccggta gaggtccatg cgtcccggag ggcagtaggt
     1501 gatgaagtgc ttctctggga tcgactggca cagtccggtc aagaggtagt gaatgtgaca
     1561 cacgcagcga cggttgactg ggtgcgcgat tcctttggtg ggtcaagcgg ctggtccggt
     1621 cgccgcgttg agctaaagag agggagcaaa tgaccaaggc aggcccggcg caatccagct
     1681 tggcgaacta tcgcgcgaag cgggagttca ccaagacgtc cgaaccagcg gggaagaagg
     1741 aggggccggc tccctcgaag cagcgcttcg tcatccaacg ccacgacgcg acgcgcctgc
     1801 actacgactt ccgcctcgaa ctcgacggtg tcttcaagtc ctgggccgtg acgcgtggtc
     1861 cctcgctcga tccgcacgac aagcgccttg cggtcgaggt cgaggaccat ccgctcgact
     1921 atggcgattt cgagggtacc attcgcaagg gtcaatatgg cggtggcacg gtgcagctgt
     1981 gggaccgtgg ctactggatt ccagaaggcg atccgcatga cgggttgaaa cgcggcgacc
     2041 tgaaattctc cttggagggc gagcggctgc acggcggctg ggtgctggtg cgtatgaaga
     2101 acgaccgcaa gggcgctaaa cggaccaact ggctgctgct caaacaccgc gacgacgacg
     2161 cccgcgatgg cgatggcgat gccctcctga gggaccccaa atcaatcgcc tccggccgca
     2221 gccttgccgc gatcgcggcc ggcaagggca aggcgcc
//
LOCUS       DASRKK010000063         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_199045, whole genome shotgun sequence.
ACCESSION   DASRKK010000063 DASRKK010000000
VERSION     DASRKK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199045"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VFC15_01245"
     CDS             <1..1867
                     /locus_tag="VFC15_01245"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZM08816.1"
                     /translation="TCIIGDGEAETGALATSWHSNKFLNPARDGAVLPILHLNGFKIA
                     NPTVLARIGRQELTDLIRGYGYEPHFVEGDVPALVHQALAGTLDTVVAEICKIQSAAR
                     SLPDTVERPRWPMIVFHTPKGWTGPKFVDGKPVEGTWRAHQVPITDFKNPEHIKQLED
                     WMQSYRPHELFDKGGKFRDEFAELAPTGHRRMGMNPHANGGELLVPLAIPHFDDYAVR
                     VSAPGSVEAESTRVLGVFLRDVMKLNLANQNFRLFGPDETASNRLEAVYEASGKEWMV
                     RIEDVDIDLSINGRVMEVLSEHLCEGWLEGYLLTGRHGLFSCYEAFVHVIDSMFNQHA
                     KWLKTSKTIPWRKPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVASKKSDVVRIYLP
                     PDANCLLSVTDHCLRSRNYVNLIIAGKQPEWQWLDIESAVRHCTVGASIWQWASNDEG
                     DPDVVMACAGDVPTLETLAAVTLLRDYIPDIRIRVVNVVDLMALEPQTEHPHGLEDRD
                     FDELFTTDKPVIFAFHGYPAIIHKLTYRRHNHDNIHVRGYKEEGTTTTPFDMVVLNNL
                     DRYQLALDAIQRIPRLRVQVEKATARYWSTMERHKLYVSEHGEDMLEVKNWRWTS"
     gene            complement(1897..2421)
                     /locus_tag="VFC15_01250"
     CDS             complement(1897..2421)
                     /locus_tag="VFC15_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08817.1"
                     /translation="MKSIAADAIRSQFYRDRQRARDSWHPRVKHRVETDNLWYVREVL
                     LHETNDRQRKGIVQWREGGCRFELPQHRFVDQAVVPKIRAPVHHAMPDRERLDLFAVC
                     EKRLDAFDRVLLGSEIRRLGNQRFIVRIFRPELTLTAADRLGLARQKQLRDGRLDPVK
                     SELERRRPAVQGKY"
BASE COUNT          560 a          757 c          760 g          545 t
ORIGIN      
        1 cacctgcatt atcggcgacg gtgaggcgga gaccggcgcg ctagccacaa gttggcattc
       61 caacaaattc ctgaatcccg cgcgcgatgg cgcggtcctt ccgatccttc atctcaacgg
      121 attcaagatc gcaaatccga ccgtgctcgc tcgcatcggg cgccaggaat tgaccgacct
      181 gatccgtggc tacggctatg aaccgcattt cgtggaaggc gacgtccccg ccttggtgca
      241 tcaagctctg gccggcacgc ttgatacggt cgtcgcggaa atctgcaaaa ttcagagtgc
      301 ggcgcgttcg ttgccggaca ctgtcgagcg gccgcgctgg cccatgatcg tgttccacac
      361 gccaaaaggc tggaccgggc cgaagttcgt ggacggcaag ccggtggaag gaacttggcg
      421 tgcgcatcag gtgccgatca cggacttcaa gaatcccgaa cacatcaagc agcttgaaga
      481 ctggatgcaa agctatagac cacatgaatt gttcgacaaa ggcggcaagt ttcgcgacga
      541 gttcgcggaa cttgcgccga ccggccaccg tcgcatggga atgaatccgc acgccaacgg
      601 cggggagctg ctggtgccgc tggcgatacc acatttcgac gattacgccg tgcgcgtttc
      661 agcgccaggc agcgtcgaag ccgaatccac ccgcgttctt ggagtgtttt tgcgggacgt
      721 gatgaagctg aacctcgcca accagaattt ccgtctattc ggtccagatg agaccgcctc
      781 caaccgtctt gaggcggtct acgaggctag cggaaaggag tggatggtgc gcattgaaga
      841 cgtcgacatc gacctcagta ttaatggtcg cgtcatggaa gtcctgagcg agcatctctg
      901 cgagggctgg ctcgaaggat acttgctcac tggccgccac gggctttttt cgtgctacga
      961 ggccttcgtc cacgtcatcg actcgatgtt caatcagcat gcgaaatggc tgaagacgag
     1021 caagacgatc ccgtggcgca agccgatcgc gtcgctaaat tatttgctca cgtcgcatgt
     1081 ttggcgacag gatcacaacg gcttctccca ccaagatcca ggcttcatcg atcacgttgc
     1141 aagtaaaaag tccgacgtcg tgcgtatcta tctgccgccc gacgcgaact gcttgctctc
     1201 ggtaactgat cactgtcttc gcagccgaaa ctacgtcaac ctgatcattg ccggtaaaca
     1261 gcccgagtgg cagtggctcg atatcgaatc cgccgttcgc cattgcaccg tcggggcgag
     1321 catttggcaa tgggcgagta acgatgaagg cgatcccgac gtggtcatgg cctgcgcagg
     1381 tgatgtacct acgctcgaaa ctctcgcagc cgtgaccctc ttgcgggact acattccgga
     1441 cattcggatc cgcgttgtga acgtggtcga cctgatggct ttggagccgc agacggagca
     1501 cccgcatggg ctggaagatc gggattttga cgaactgttt acgacagaca agccggtgat
     1561 cttcgccttc catggctacc ccgcgatcat tcacaagctg acctaccgac ggcacaatca
     1621 tgacaatatt catgtgcgcg gctacaagga ggagggcacc accacgactc ccttcgacat
     1681 ggttgtgctg aacaatctcg atcgctatca actcgcgctg gacgccattc agagaattcc
     1741 acggctgcga gttcaagtgg agaaggcgac ggcgcgttat tggagcacga tggagcgtca
     1801 caagctttat gtcagcgaac acggtgagga tatgctcgag gtgaaaaact ggcgctggac
     1861 ctcataagcc gtatggcaag cgctggacca cgttcatcag tacttgccct gaactgcggg
     1921 tcgtcgtcgc tcaagttcgg actttaccgg gtcgagtctt ccatcccgaa gttgtttctg
     1981 tcgggcgagg ccgagtcgat cggcggccgt cagggtaagt tctgggcgaa agatgcgaac
     2041 gataaaccgt tggtttccta atcggcgaat ttcggaaccc agcaggacgc ggtcgaacgc
     2101 atcgagacgt ttctcgcaga ctgcaaagag gtccaaccgc tcgcggtcgg gcatcgcgtg
     2161 gtgcacgggg gcccgaatct tcggcaccac tgcctggtcg acgaaacggt gctgcggcag
     2221 ctcgaagcgg caaccgcctt cgcgccactg cacaatcccc ttgcgctgtc ggtcattcgt
     2281 ttcgtgcagg agcacttccc gcacgtacca caagttgtct gtctcgacac ggtgtttcac
     2341 tctgggatgc cagctgtcgc gcgcacgctg cctatcccta tagaactgcg atcggatggc
     2401 atccgccgct atggatttca tggcctttct tgcgagtcga tcatgcgcca acttgggagc
     2461 aatcggccgg atcgactagg gcgcgtactc ataaatcgaa gctcattggt gcggcttatt
     2521 cgatgtccgc tatgccccga tagcgaccaa actctgtagc gcagcgaaat gtcgcaaagt
     2581 accggaagct gccatgtgac cgtgaccggg ctcgaatgca ga
//
LOCUS       DASRKK010000064         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_296688, whole genome shotgun sequence.
ACCESSION   DASRKK010000064 DASRKK010000000
VERSION     DASRKK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296688"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFC15_01255"
     CDS             <1..971
                     /locus_tag="VFC15_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZM08818.1"
                     /translation="AEVALGVASVDIVGVDDAEPAVKDPYEILGVAPTASADDIQKAY
                     RKLAKKLHPDLNPGNAEAEEKFKEIAGAYGLVGDAEKRTRFDSGEIDAAGAERPRQNY
                     YRDYASSEQDHPYTDNSGFADFMDSEDAFAELLRRSAQAQANRRGHDLAYRLPIEFIE
                     SIAGANKRLTLPDGGTLDVAIPPGITEGQVLRLRAKGAPGSGKGGFGDALIEVAIKPD
                     RSFTRQGDDIYLELPITLTEAVLGARIKVPTPTGAVTMAVPKGSNTGTTLRLRGKGAP
                     KHAGGYGDQMVKLKVVLPKEPDPELEAFVSNWQSGKTHDPREEVTP"
     gene            968..1255
                     /locus_tag="VFC15_01260"
     CDS             968..1255
                     /locus_tag="VFC15_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZM08819.1"
                     /translation="MKISKEEFLSLSGMGLKMLNAWIEEEWLIPSGSSPELSFSEMDI
                     ARARLIRDLQVDFGVNDEGVGVILNLVDQLYGMRRTLMELRQEVRITTEQG"
     gene            1449..>2057
                     /locus_tag="VFC15_01265"
     CDS             1449..>2057
                     /locus_tag="VFC15_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZM08820.1"
                     /translation="MATRRMASLSSRKVIYAAMVGNLLVAATKFGAAAWTGSSAMLSE
                     GVHSVVDTGNSLLLLYGLHRAKKSPDHDHPLGYGREIYFWSFVVAVMVFALGAGVSFY
                     EGVVHVLYPEPIRNTAISYAVLGLSALFDGTTWWIALRNFKGRMKFSSLVGAIHNSKD
                     PPSFMVLFEDSAALIGLIIAFAGTYLVVRFDLPILDGIASIMI"
BASE COUNT          461 a          528 c          600 g          468 t
ORIGIN      
        1 aagctgaggt agcccttggc gttgcaagcg ttgacatcgt tggggtcgat gacgcggagc
       61 cggcagtgaa agatccatat gaaattctgg gagtcgctcc gacggcttca gcggatgata
      121 ttcaaaaagc ttaccgtaaa cttgcgaaaa agctgcatcc cgacctcaat cccggaaatg
      181 cggaggcgga agagaaattt aaagagatag ccggtgccta tggcctggtc ggcgacgctg
      241 aaaagcgcac gagatttgat agtggtgaga ttgacgccgc cggggccgaa cgacctcggc
      301 agaattatta ccgggactac gcgtcgtcgg aacaggatca tccttatact gacaattccg
      361 gtttcgcaga tttcatggat tcggaagacg cttttgctga acttctgagg cgaagcgccc
      421 aggcgcaggc aaacaggcgc gggcatgatc tagcgtatag gctcccaatt gagtttattg
      481 agtcaatcgc cggcgcgaac aagcggctga cactgcccga cggcggtaca cttgatgtag
      541 ccatcccacc cggcattacc gagggtcaag ttttgcggct gcgcgccaag ggtgcgcccg
      601 gatcaggcaa gggtggcttt ggggacgctc tcattgaagt cgcaatcaag ccggaccgtt
      661 ccttcacccg ccagggagac gacatctatt tggaattgcc gattaccctc acagaggcgg
      721 tcctcggtgc ccggatcaag gttcctacac caacaggtgc ggtaacaatg gccgtaccga
      781 aagggtcaaa taccggcacg acgctacgtc tgaggggcaa gggcgccccc aagcatgccg
      841 gtggttacgg cgatcagatg gtcaagctga aagttgtact tcccaaagag ccagatccag
      901 agctcgaagc cttcgtctca aactggcaaa gcgggaagac ccacgacccc cgtgaggagg
      961 tgacaccatg aagatcagca aagaggaatt tctatcgctc tccggcatgg ggctcaaaat
     1021 gctgaatgcc tggatcgagg aggaatggct gatacccagc ggttcgtccc ccgaactgag
     1081 cttctccgaa atggatatcg ccagagcgcg gctcatccgt gatctccagg tggattttgg
     1141 cgtgaatgat gaaggagtgg gggtaattct gaatttggtc gaccagctct acggcatgcg
     1201 caggacactg atggagctgc gtcaagaggt ccggattacg actgaacagg gttgagactt
     1261 gaccgtcgct aaaattcgca ttgtagcctt ggcgtaacga cgaggagcct cactcttcgg
     1321 tttcgatact tttcagctcg ccgcgaccaa acgctttgag atggtcgttt gcaatcgaaa
     1381 ctatggcggt gagggagtgt cccgatcgac tgttttctaa gcagcgcgcg gtcaccagag
     1441 gatggttcat ggctactcgc cgaatggcgt cgctttcgtc tcgaaaagtg atttacgcgg
     1501 caatggttgg aaatcttctg gttgccgcaa cgaagttcgg tgcggccgca tggacaggca
     1561 gttccgccat gttgagtgaa ggtgtccact ccgttgtcga caccggaaac tcgcttctgc
     1621 ttctttacgg tctacatcgc gcgaaaaagt cgcccgatca cgatcacccg ctggggtacg
     1681 gccgtgaaat atatttctgg agtttcgtcg tcgccgtgat ggtttttgcg ctcggcgccg
     1741 gggtgtcctt ctatgaaggc gtcgtacatg tcctatatcc cgagccgatc cggaatactg
     1801 caatcagcta cgcggttctt ggtttgtcag cgctgttcga tggtaccacg tggtggatcg
     1861 ctctacgcaa tttcaagggc cgaatgaagt tttccagtct tgttggagca attcataata
     1921 gcaaggatcc gccgtccttc atggttctat ttgaggatag cgcggccttg atcggtctca
     1981 tcattgcgtt cgccgggacg tatctcgtgg tcagattcga tcttccgatc ctcgatggca
     2041 tcgcatcaat tatgatc
//
LOCUS       DASRKK010000065         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_785614, whole genome shotgun sequence.
ACCESSION   DASRKK010000065 DASRKK010000000
VERSION     DASRKK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785614"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(554..1723)
                     /locus_tag="VFC15_01270"
     CDS             complement(554..1723)
                     /locus_tag="VFC15_01270"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM08821.1"
                     /translation="MRIVALDLGAKKTSYCELSQGQVVHRTTVSEVESLRPLLGPDQP
                     AAKVAIEACREAWFVHDLLVEWGNEVVLVDTTRSRQLGIGQHGRKTDRIDAETLARAL
                     EAGRIPKAHVLSPKRRELRRVLGVRRALVEARAQLVTTVRGLVREQGGKIPSCTTGNF
                     VSRAREQKLASCIAELIEPLLVLAQGIDTQLANTDEQLAVLCAQEPIIALLTTTPGAG
                     TVVAASFMSVVDEAKRFHSAHQLESYVGLVPSEDTSGGKRRLGAISKKGNCYLRSLLV
                     QAAWTIVRSSDKSDPLYLWAKKLEQRRGKRIAVVALARRLVGVLWAMWRDGTVYDAQH
                     LAQQGVRGLHSAVQSLELQKEKLAQAAKKESVKHPHIQSTAKARRSSKTPAVKAA"
BASE COUNT          371 a          660 c          641 g          498 t
ORIGIN      
        1 atccggatca gcaaccagca ccgggtcacc ccatgtcccc gtgccgtcac tacacaccta
       61 gcggagaggg agggattcga accctcggta cccttgcagg cacaccttgg tggtgcctcc
      121 acccgttcgg ccgcggctgc gctgcgacct cccgacctcc tccaactatg agtcgggccc
      181 aacgtcaact gttttgtctt tcttttttct tcgactgtgg tctgccgggt ggtggcgatc
      241 ttggatgcag ttgcgttcga catggagctc tcctgtggct gcgctgcagc cagtgtcgga
      301 actaggttgg ggtcgttgcg cgggcgagcg gggaagcgac gagctggtgt cgatcaacta
      361 ttcgcactta gggaaccgac ctggtttggt tattatggtt ccggtgcatt tgggggtccg
      421 aagcgggttg gtgacggcgc attctacgcc acgggctcag tcgacttctg ccaacggcca
      481 ttggaggagt cgcgctcagt caccgaccga tctgtgctgc tctagctttc acgctcttgg
      541 cgcagtttgg aggtcaggcg gctttcaccg ccggagtttt tgaagaacga cgagccttgg
      601 ccgttgattg aatgtgagga tgcttaacag attccttttt ggcggcttgc gccagttttt
      661 ccttttgaag ctcaaggctt tgaacggcgc tgtgcaggcc acgaacaccc tgttgagcga
      721 gatgctgggc atcgtacacc gtaccatcgc gccacattgc ccaaagcacg cccaccagac
      781 gtcgcgctag agcgacgacc gcgatccgct taccacgacg ttgttcgagc ttttttgccc
      841 aaagatacag tgggtcactt ttgtcgctgc tacgcacgat ggtccacgcg gcttgtacta
      901 acaaggagcg caagtaacag ttcccctttt tggagatggc gcccaaacgg cgtttgccgc
      961 cgctggtgtc ttcgctgggc actagcccca cgtaggactc gagctggtgg gcactatgaa
     1021 accgcttggc ttcgtccact acggacatga aagaagcggc aaccaccgtg cccgctccgg
     1081 gcgttgtcgt cagtaatgcg atgatgggct cctgtgcaca gagcacggcc aattgctcgt
     1141 cagtgttcgc taattgagtg tcaattcctt gcgctagcac gagcagtggc tcgatgagct
     1201 cggcgataca cgacgctagc ttttgctccc gggccctact gacgaaattc cccgtggtgc
     1261 agctcggaat ctttccaccc tgctcgcgca ccagcccgcg tacggtcgtg acgagttgcg
     1321 ctcgcgcttc aaccaaggca cgtcgcaccc ccaacacccg tcgcaactca cgtcgtttcg
     1381 gcgacagcac gtgtgctttg ggaattctgc cggcctccag cgcacgagct agtgtctcag
     1441 catcaatgcg atcagtcttg cgcccatgct ggccaatccc cagctgccgg ctgcgtgtgg
     1501 tatctaccaa tacgacttcg tttccccact caaccaaaag gtcgtgcacg aaccaagcct
     1561 cgcggcaggc ctcgatggcg actttcgctg ccggttggtc cggtccgagc aacggccgca
     1621 gcgactcgac ctcgctgacc gtggtgcggt gaacgacctg tccttgggac agctcgcaat
     1681 aactggtctt ctttgctcca agatctagtg ctacaatacg catggtcatc ctgccttcgg
     1741 ccccttggga aatttgcgtc tctgaacgcg ttggggcgcc gtctggattt tcgccggacg
     1801 gcggcagggt ggccctattt tctgcaactg tgtgtacgtc aacgggcaaa gttgctgggt
     1861 ctgcttgccc agaccggcgc cgggcacgtc gcttcgcggt cgggacgtcc actcgatccc
     1921 gagatgcaag cgccgtgacg ggcgccgcgc acttacgctt caggtttgtt ctgcttttgg
     1981 tgatgggcgg catgacagct gcggctccct ggcccgactc atcccatcta cgccatgctc
     2041 ggtcgcctcc ggcgccctgc gcgtggcggg aaggaggtgg tttagtaggg ttgactcgcg
     2101 aactttcgcg ttcgctcgag ttactgaccg agcctgattg gcggggggta gactcagagt
     2161 acacctgcag
//
LOCUS       DASRKK010000066         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1078695, whole genome shotgun sequence.
ACCESSION   DASRKK010000066 DASRKK010000000
VERSION     DASRKK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078695"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(889..1293)
                     /locus_tag="VFC15_01275"
     CDS             complement(889..1293)
                     /locus_tag="VFC15_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08822.1"
                     /translation="MAPAEQATPTYLGRDSLFLEAFRDLSVLAQDPTWAFFRYCAKLK
                     TAPTVALSVIVTLQTQDSASDSLLAVVHELGSGTTGTQLAENPPNFDPVDGLAVRVTG
                     APSTKCPVQPPPEPQLIPPGELTTFPVPWPKS"
     gene            1610..1686
                     /locus_tag="VFC15_01280"
     tRNA            1610..1686
                     /locus_tag="VFC15_01280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1644..1646,aa:Arg,seq:cct)
     gene            complement(1836..3188)
                     /locus_tag="VFC15_01285"
     CDS             complement(1836..3188)
                     /locus_tag="VFC15_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HZM08823.1"
                     /translation="MVGRRQPIEQWDANLWELNTPGGIVDLLTGGLRAATPEDYCTKI
                     AGSAPGGECPRWVEFLDRVTGGNLELQQFLQRMVGYGLTGSTREHSLFFFYGTGANGK
                     SVFLNTIGELLGDYSKVAPVASFTATTTEQHPTDLAGLRGARFVSAIETEDGRWWAEA
                     KIKSLTGGDKITARFMRQDFFEYVPQFKLIVAGNHKPGLRNVDEALRRRLHLVPFVVT
                     IPEGERDPQLCEELRTEFPGILQWAIEGCLAWQRDGLSAPEIVKNATADYLAGEDAIA
                     RWLDERCVVDSKCWSSSTALYTDFKGWCQEAGERERTLKAFTQALEDRKFPRERTRVA
                     KGFSNIGLKTDTVPDVPRQPHIPVTRARAYTSYIGGPGTCGTIPRHVPRCAGLPKRED
                     RAPDVAGCVPAETNPADRQDSRDNGAGNVPGASPVLYDAEPEEWLTERRAGSHGPRLN
                     "
     gene            3583..3840
                     /locus_tag="VFC15_01290"
     CDS             3583..3840
                     /locus_tag="VFC15_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08824.1"
                     /translation="MLHSSHKVCDAWTFDQTVSQLSDVGSAADVLKLIVATTFTAERG
                     LVDRPVGFGQVPHAAENDPVGGERELRLAQHACFTPKGVSQ"
     gene            complement(4035..4471)
                     /locus_tag="VFC15_01295"
                     /pseudo
     CDS             complement(4035..4471)
                     /locus_tag="VFC15_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(4857..>5069)
                     /locus_tag="VFC15_01300"
     CDS             complement(4857..>5069)
                     /locus_tag="VFC15_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08825.1"
                     /translation="LLLLLNLNSYRLGFTVFGPGYRNYVSQIEHYILSYTPSSEKPFP
                     HPGDRDIDAEMIQFLRDKHHPLFAAH"
BASE COUNT         1036 a         1501 c         1392 g         1140 t
ORIGIN      
        1 gccgacggcg gcccactgat ctacaacggc tcggaagagg agcaacgctc cctcgattcg
       61 acctgccgca aactccaccg cttcctcaat caaccccagc gctgagcccc aaaacaccca
      121 cgaatccttc ggacgaggct aaattcccaa aagcccgtcc caagagatct agaaaggaag
      181 aaatcaaatg actggtcgct ccattttgcg ccttccgcta cttatctgtt cgctactact
      241 tgtctgtatg ctactacttg caggctgcgg ttcaaccccg tgggtatctt cgatgcaggt
      301 tatacccgcg agtcaaactc tccagggcgt gggagctacg gtacaataca aagcgatggg
      361 cacgttaacg aatcgcgagc atccgcaaac ctacaaagac gttacgaatc aggtggcctg
      421 gtcttcaagt gttgagagtg tcgcgacgat cagttctgtg ggattggcta cgggagtgag
      481 tgcaggcacc acgactataa cagcgactgc tggcggtacg gcagcgacag cgaccctggt
      541 tgtggggaat ggaaccggct catcacacga tctcacttcg ttgacgatta tccctgggga
      601 tcaaatcgtc aaccttgtag gggagactgc acagtacatc gcgataggaa gtttcactgg
      661 cactccggcg gcccaggata tgacgaacca ggtgacatgg tcctcaagcg atgtgcgcat
      721 agcaaccgta aatgccgctg ggctggcaac ggccgtcggc aattgtgatg gccaagatca
      781 agtaaccaca atcacggcgc ttgcgacctc cggcggggtg gcaattgctg gaacatctga
      841 ttttacggtg gacgtttgcg gcacaaacaa cctgccctct ttgaccgtct acgatttcgg
      901 ccaaggcacg ggaaacgtcg tgagttcgcc agggggaatc agctgcggct cgggtggcgg
      961 ctgcacggga cactttgtgc tgggtgcgcc cgttactctc acggcaagtc cgtcgacggg
     1021 atcgaagttc ggcggattct ctgccaattg tgtccctgtc gttccggacc ccagttcgtg
     1081 taccaccgcc aacagggagt cggacgcact gtcctgcgtc tgcaatgtga cgatgacgga
     1141 caacgcaacg gtgggagcag tctttaactt ggcacaatag cgaaagaatg cccacgtcgg
     1201 atcttgggcg agcacgctca agtcgcgaaa tgcctccaag aacaggctat cccgtcctag
     1261 ataggtcggg gtagcctgtt ccgctggtgc cactctgtcg ccgcagccat ttcgttccta
     1321 tgcctcatgt tagagccgct gctcatcaga tctagggtga atgggtaatg ggcaggggat
     1381 ccctttgcac ggtctgactg acatcgccac atggacacaa aaattaccga ctattacact
     1441 agtgtccggg acaatccaaa gacgaaggcg gtagcggcaa ttgagcaggc gcccccccaa
     1501 ctgctcgccc tgctgggaat cgagaacggt acagagggaa aaatccaata atggctggct
     1561 ttgtgggcga ttctgtggat gattgacagt tggtgccgta agtccttgtg gccctgtagc
     1621 tcagatggat agagcagcgg tttcctaaac cgttggtcgg cagttcgact ctgcccaggg
     1681 cccccatttt caaccacttg cacgtgcgcc ctctgcggtt tgtgttcagt agtgttcata
     1741 atcactgtat gcttcccatc acgtctgtca cgtcgagcgc gaccatctgc gcgacggagt
     1801 ctggaatctc catcatgtag acgtttcccg ttgtctcaat tgagccgtgg cccatgtgaa
     1861 cctgcacgtc tttcagtgag ccattcttct ggttccgcgt cgtacaggac cggcgaagca
     1921 cctggaacgt taccggcacc gttatcccga gagtcttggc gatcggctgg attcgtttct
     1981 gcaggaacac acccggccac atcgggcgcc cggtcttctc gcttgggaag accagcgcat
     2041 cgggggacgt gtcgtgggat tgtgccacat gtgccgggtc cacctatata ggacgtatac
     2101 gcgcgcgcgc gtgtaacggg tatatggggt tgacgtggca catctggcac agtgtcagtt
     2161 ttcagcccga tattggaaaa acccttggct acgcgagttc gctcacgcgg gaatttgcgg
     2221 tcctctagtg cctgcgtgaa ggcctttagt gttcgttcgc gttcgccagc ttcttgacac
     2281 caacccttga aatcggtata gagtgcggtt gacgaagacc agcactttga gtccaccacg
     2341 cagcgctcat cgagccatcg ggcgattgcg tcttcgcctg cgaggtagtc cgcggtcgca
     2401 ttctttacaa tctcaggtgc gctcagtcca tcgcgctgcc aggcaagaca gccctcaatc
     2461 gcccattgca ggatgccggg aaattcggta cgcagctcct cacacaactg cggatcacgc
     2521 tcgccctcgg gaatcgttac cacaaacggg actaagtgca gtcggcggcg cagggcttca
     2581 tctacgttgc gcaaaccagg tttgtgattt ccggccacaa ttaatttgaa ctgcggaacg
     2641 tactcgaaaa agtcctgcct catgaatctg gctgtgatct tgtcgccccc ggtgagcgac
     2701 tttattttgg cttctgccca ccagcgccca tcttcggttt caatcgcact cacgaatcga
     2761 gcgccgcgaa gcccggccag gtctgtcggg tgctgctcgg tggtggttgc cgtaaaggaa
     2821 gcgaccggtg ccaccttgct gtagtctccg agtagctcac cgatcgtgtt caggaaaaca
     2881 cttttcccgt ttgcgcccgt cccataaaag aagaacaaac tgtgttcgcg cgtgctaccg
     2941 gtaagaccat accccaccat gcgttgaagg aactgctgca attcaaggtt cccgcccgtg
     3001 acgcgatcga gaaactcaac ccaccgcggg cactcgcccc ccggagcgct gcctgcgatc
     3061 ttggtgcagt aatcttcggg cgtggcggct cggaggccgc ccgtgagcaa atcgacgata
     3121 ccgccgggag tattcaactc ccacaggttg gcatcccact gctctattgg ttgccgcctg
     3181 ccgacgatcg cagcgcgcca gcctctcgac cgcggtaacg gtctgggctg aagtgaggcg
     3241 gatcgcctcg cgctgcttaa cgtcgttgtc tacgcactct tggctcgcgg cgcaacaaat
     3301 gtcacgcact ttcgcccaca cgtgcaacgt tgcgtcctcg gaccaccggc agccatccca
     3361 gcccatccac tttccccaca tattgacata acgaagatcg tcgacatgtt cagccgaaaa
     3421 ccgcattgcg agtgcagtct cggagaaccg cggaggtagc ggcgcctctc ctacgttatt
     3481 gacctcggca acgaggtgcg aagcgatttc actcgctgct tggggagtag gggcactctg
     3541 tatgtcttca ccggtcttcc gcgccaggcg gaggagtgcg gattgctgca ctccagccac
     3601 aaagtctgcg atgcttggac gttcgaccag accgtcagtc agctcagtga tgtaggcagc
     3661 gccgccgacg ttctgaagct cattgtggcg accacattca ccgctgagcg tggcctcgtc
     3721 gataggccgg ttggattcgg ccaggtcccg catgctgcgg aaaatgatcc ggtgggcgga
     3781 gagagagaac tgcgactcgc acaacacgct tgcttcactc caaagggagt ttctcagtag
     3841 cacgctgccg agtacgcagc gctcaactct ggcgctatcc atctctgcac tttacgctcc
     3901 tattagctaa ggtcaaggtt actgccgtac ccttgcgtac ccttgcgtac ccttttttcc
     3961 cctcgtgtga ttgcttccga ccatcaaccc tatctccgcc ggcgacgggt ccagtggcgg
     4021 gtctaggatt ggaaccggtt ggcgttcttt ttcaatcctc gatagcgcac tttcacaaac
     4081 ccaaacttca gcttcattac tccaaacacg tgctccactt tcgagcgcac tcgcgacttg
     4141 gtacggtttt tggcccgttc tatttcgtcg acatggtctt tgtagcggta ctgcttgtgg
     4201 gtcatgtcct gtgccccggg tgcaacttcc tgcatggcct ccgtctgccc ccgataggcc
     4261 tgatcgcccc aaaccttcgt ctcttccccg tgcagcaact ccggcaatac cgtgctatcc
     4321 gccacgttgg ccgccgtcac caccacactg tgaatcatct tcgtcttact atctacccct
     4381 acgtgggctt tcatcccgaa gtaccactgt gcttgccctt cctcgtttgg tgcatcgcag
     4441 ggtcgcgctt ctgctcccgg ttcttggtcg aactggcggc taggtccttg ctcaacgtgc
     4501 tgtgctcgcg atacgtggcc ctgtggcaag tttgccgcct cctagtacgt cgggacaccc
     4561 aaacgccgca ggagcgccat gcagcaagca acaccagact aagtgcttcg cccttggact
     4621 gatcgctctc tgatctgctc gcctgttccc ttatatcaaa ttgagaacct ataaaccgcg
     4681 aaaggcgatt tggggcgtgc agccgacacc gttacacggt tccttggtga agctgtcgtg
     4741 ggggactatc cagtccgtcg tctcgaagcg cgcatcgttg atactctgcc cgatgcaaaa
     4801 gccatccagc ggacattccc gcattcggaa gcatggttcg ctcgaaccaa cgaaccctag
     4861 tgtgccgcga aaagcggatg atgcttgtcg cgaaggaact ggatcatctc ggcatcgatg
     4921 tcgcgatcgc cgggatgggg aaacggcttt tccgacgaag gcgtgtatga gaggatgtaa
     4981 tgctcgattt gactgacgta attgcggtaa cccgggccaa acacggtaaa gccgaggcga
     5041 tagctgttaa ggttcaatag gagtagaag
//
LOCUS       DASRKK010000067         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1274349, whole genome shotgun sequence.
ACCESSION   DASRKK010000067 DASRKK010000000
VERSION     DASRKK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274349"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..729
                     /locus_tag="VFC15_01305"
     CDS             280..729
                     /locus_tag="VFC15_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM08826.1"
                     /translation="MRSETVLLVEDDMLVARFLMLVMRMWGYDATHVSTSREAVDFAI
                     LHQNEITLTLCDVNLQGESGLSVAAKIRGLCPRSKTIFTSGFPLDVLCEGGLLTQEVL
                     QNGDTGYIQKPFLPGDLSNAIYSLLEPAAEVPWAASQTETRYASAAC"
     gene            710..1303
                     /locus_tag="VFC15_01310"
     CDS             710..1303
                     /locus_tag="VFC15_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM08827.1"
                     /translation="MPAPHVNDELLDQYALGTLPEERVAGVEEHLLVCEACQSRLDAS
                     DEFAMLFRAASVQPDARPQRSWRAFWNHRAANWTVASAAMLAILLLVAGPFREPQMAP
                     ATVLMQSLRGPDAPAQVAAGRPALLVFDLVPSPGVNEYEARIVNPVGVEILAAKVSSK
                     DGRLAVLVERLPPGSYWVRVFRSDNREPIAEYGLRAR"
     gene            1266..2489
                     /locus_tag="VFC15_01315"
     CDS             1266..2489
                     /locus_tag="VFC15_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM08828.1"
                     /translation="MNRLRSMVCEPGSARKNLEEQLRQVQKMEAIGRLAGGIAHDFNN
                     LLTVISGYSEIVLSTLSAEDPMRESIQAISDAGVRAASLTRQLLTFSRQRELEPRVLN
                     LNEVVREVERMLRRLIGEDVLLTSVLDPAIHNVKIDPALFEQVLMNLAVNARDAMPTG
                     GKLTIETCNVNWDRDYAATHPQIQTGPYVMLAVTDTGSGMPPEARTRIFEPFFTTKEA
                     GKGTGLGLAVVDGIIKQSNGHIEVCSEPDVGTTFKIYLPAVAEQVAVPKALDSGQAIG
                     GAETILLVEDEKAVRGLALQTFQSNGYQVLTAADGRDAMLVAEEFRGPIDLLMTDVVM
                     PGMGGRQLAESLRSAFPKMKVLYTSGYTDDTVVRQGIFRKEVAFLQKPYTPLSLTRNV
                     RAVLDGTTCVATSEQ"
     gene            2729..2923
                     /locus_tag="VFC15_01320"
     CDS             2729..2923
                     /locus_tag="VFC15_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08829.1"
                     /translation="MDESEFDVFRVLEELTDPKVEIPFDEDAPFTGDPEALTEFQVQV
                     WCSCQTGVKPCIAHLPDSKT"
BASE COUNT          671 a          853 c          944 g          636 t
ORIGIN      
        1 gcagaccccg cagtggcggc aaagatggca agcatcactt cgataatcgt agacatgtgg
       61 attcctttcc gaacatcggc tattcgcacg ggtgttttgc gttctcgtac gaaaggacac
      121 aatactgcga aattcgactc cagaactatc ttcaaattgg gtagtcccgt attatcaaca
      181 atttaggcat aaaatacgta actgacgata gcgcaaattg ttaacttccg gtgataagaa
      241 tcccgcgccg tgcgcactgc tcataaagag gatttattca tgcgctccga aacggttcta
      301 ctggtggaag acgacatgct cgtcgcccgg ttcttgatgt tggtgatgcg gatgtggggc
      361 tatgacgcta cccatgtgtc tacttcccgg gaggccgttg acttcgcaat acttcatcaa
      421 aatgaaatta ccctgacctt gtgcgacgtc aacctgcagg gcgagtcggg gctttcagtt
      481 gctgccaaga tccgcggtct ctgcccccgc agcaagacga tcttcacctc aggatttcca
      541 ttggacgttc tttgcgaggg tgggctcctg acacaggaag tactacagaa cggcgacact
      601 ggctacatcc agaagccttt cctgcccggg gatctgagta acgcgattta tagcctgttg
      661 gaacctgcag cggaagtccc atgggccgca tcgcaaacgg agactcgata tgccagcgcc
      721 gcatgttaac gacgaacttc tcgaccagta cgctttaggc acactcccgg aggagcgtgt
      781 ggcaggcgtg gaggaacacc tgctggtctg cgaggcctgt cagtcgcgtc tggatgcatc
      841 ggatgagttc gcgatgctat ttcgggcggc gtcggtccag cccgatgccc gtccgcagcg
      901 cagttggcgg gcgttttgga atcatcgtgc ggcaaactgg acagtagctt cagcggcgat
      961 gttggcgata ctgcttttgg tggcggggcc gtttcgtgaa ccccaaatgg cgccggctac
     1021 ggtgctgatg caatcacttc gcggacccga cgcgccggca caagttgctg ccggccggcc
     1081 agccttgctg gttttcgatc ttgttccgag cccgggagtg aatgagtatg aggctcgcat
     1141 cgtaaatccc gtaggggtcg agattctcgc agctaaggtt tcctcaaagg atgggcgtct
     1201 ggcagtgctc gtggaaagac tgccgccggg atcctactgg gtccgtgtct ttcggagtga
     1261 taaccgtgaa ccgattgcgg agtatggttt gcgagccagg tagcgcccgc aagaacttgg
     1321 aggagcaact gaggcaggtc cagaagatgg aggctatcgg ccggttggcg ggcggaattg
     1381 ctcacgactt caacaacctg ctaacggtca tctccgggta tagcgaaatc gttctgtcga
     1441 cgctatcggc cgaggaccca atgcgggagt ccatccaagc catcagcgac gcgggagtac
     1501 gtgccgcgtc gctcacacgt cagttgctca ccttcagccg ccagagggag ttggagccga
     1561 gggtgctgaa cctgaacgaa gtggtgcgcg aggtggagag gatgctccgg cggctgatcg
     1621 gtgaggacgt tctgctgact agcgttctcg acccggctat ccacaatgtc aagatcgatc
     1681 cggctttgtt tgagcaggta ctgatgaacc tggccgttaa cgctcgtgat gcaatgccca
     1741 cgggcgggaa gttgaccatc gaaacctgta acgtgaactg ggatcgggat tacgccgcga
     1801 cccatccgca aatccagacg ggcccgtacg tcatgctcgc ggtgacggac accggctccg
     1861 ggatgccgcc cgaagcgagg actcgcatct tcgagccgtt cttcacgaca aaggaagcag
     1921 gcaaaggaac aggtctgggg ctggctgtgg tcgacggcat tatcaagcag agcaatggtc
     1981 acatcgaggt ttgcagcgag cccgatgttg gcacgacctt caagatttac cttcccgcgg
     2041 tcgcggagca agttgccgta ccgaaggcgc tcgacagcgg ccaggcgatc ggtggggcag
     2101 aaacgatcct actggtcgaa gatgaaaagg cagtgagagg actggcccta caaaccttcc
     2161 agtcgaacgg gtatcaagtt ctgacagcgg ccgatggcag agacgcaatg ctggtcgccg
     2221 aggaattccg cggacccatc gatctgctga tgacggatgt ggtgatgccc ggcatgggtg
     2281 gccggcaatt ggctgagtcc ttgcggtccg cgttcccgaa gatgaaggtg ttgtacacca
     2341 gcggctacac ggatgacacc gtagtccggc agggcatttt tcgaaaagag gtggcttttc
     2401 tgcaaaagcc gtacacgccg ctttcgctaa cgaggaatgt tagagcggtg ctggatggga
     2461 caacctgcgt ggctacatca gagcagtagc gaatccacag atgggggcac ggcgccggga
     2521 cgtgcgttcc cggtgctctg tgtctctcct cggtgccacc gaaacccctt gtgcaagaat
     2581 agagacgttg tttctttgag gttcgggctg tagcgcagcc tggtatcgcg cttgcttggg
     2641 gtgcagctct ccgaatgcaa cggagcagtg cttctatcat tggcgcgaga agttcgggcg
     2701 catcaacatc gagaaaatca aacatccggt ggacgaatcc gagttcgatg tgttccgcgt
     2761 gctggaggag ttgaccgacc cgaaagtcga gatcccgttc gacgaagacg cccccttcac
     2821 cggcgacccg gaggccctga cggaattcca agtacaggtc tggtgctcct gccagaccgg
     2881 tgtcaagccc tgcatcgccc acctgccaga cagcaaaacc tgaacggctg gccgaaacag
     2941 cgatcgccag tagatcagta tccccgccgc gaatcacaag ccaaatagtc cccaccccct
     3001 gccagacagg agttcaagga atccccatcc tccatcagag ccgcccggcg cgaggactca
     3061 gcggtcaggc agaccgaaat aggactgctg gccgcgacct gggt
//
LOCUS       DASRKK010000068        16087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_296876, whole genome shotgun sequence.
ACCESSION   DASRKK010000068 DASRKK010000000
VERSION     DASRKK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296876"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFC15_01325"
     CDS             complement(<1..1023)
                     /locus_tag="VFC15_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM08830.1"
                     /translation="MNIAEAIRIALLSLWANKLRSVLTLLGVVIGVAAVIAVVTFVNG
                     INGYVAEKIFNLGTDVFIISKTPNVITDVDQFIEGQKRKDLTLEHYQAVADGCDACRI
                     VGAAVRQFNGKVVYGTQSSTDTMVRGWTPTMAQIYDLDLVAGRMLNNADENTTSAVVI
                     IGQDIRENLLADTDPLGKEIRVDGKVYRVVGVGKKEGKTLGQSRDNYVIMPITTWLKN
                     YGAHNSLNIWAKADDVGTSFEQAIDQARVILRSRRHDRVGEKDSFVIETNASFLSLWS
                     DLSGTFFIVMIVIASISLIVGGIVIMNIMLVSVTERTREIGIRKALGARPRDVLRQFL
                     IESITMA"
     gene            1436..2137
                     /locus_tag="VFC15_01330"
     CDS             1436..2137
                     /locus_tag="VFC15_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HZM08831.1"
                     /translation="MKAKEDKIASSPTSKELVVFSILRESRCKECNKELWKGNFLFME
                     ADRPLCLSCADLDHLTYLPRGDAALTRRAKKYSPLFAVVVRFSRSRGRYERQGILISE
                     AALEQAEEECLADTEQRARRRERDEVRRAEYDRDLIVHMAGVILKLFPGCPPKEARTI
                     AEHTARRGSGRVGRTSAGQSLEEGALTAAVIASIRHRHTRYDELLMSGYSRTDARGSI
                     REDLDHVLKTWRYPP"
     gene            complement(2526..4001)
                     /locus_tag="VFC15_01335"
     CDS             complement(2526..4001)
                     /locus_tag="VFC15_01335"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZM08832.1"
                     /translation="MYIVEIASECAPAAKAGGLGDVVNGLSRELQTRTQWVEIILPLY
                     DCMRYDRMGPLEKVYHDLWVPWYGGSIHCSVFFATVDGLRCYFIEPHSPDNFFRRGHF
                     YGSPDDIMRFVFFSRAALEFLLKSDRRPDIIHCHDWQTGIVPVQLFEIYKFLGMGNSR
                     VCYTIHNFKHQGVWDAEVLKASGLNRPEFFMHRDRMQDDFNHSALNLLKSGIVYSNFV
                     TTVSPQHAWEARFTEQGFGMGPTLAVHQTKFAGVLNGLDYSVWNPEIDPCIPVHYGPG
                     NLDSKYADKQELRNRLGLAKEFKPLISFVGRLDPQKGIHLIQHALHYALGHGAQFVLL
                     GSSPDPGINNLFYNLKRYLQDNRDCHLEIGYREELSHLIYAGSDIFIVPSMFEPCGLT
                     QMIALKYGTVPVVRAVGGLVNTVFDHDYSDKPGEARNGYVFHQADPAAIESALSRAIG
                     LWNNYPEEFRSLILNGMRYDYSWRIPGERYLDIFHGISSGW"
     gene            complement(4015..6408)
                     /gene="glgB"
                     /locus_tag="VFC15_01340"
     CDS             complement(4015..6408)
                     /gene="glgB"
                     /locus_tag="VFC15_01340"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZM08833.1"
                     /translation="MPSHDPLSPPSAVAPVTPAEADRKDSSAAADNASPPGADRKDSP
                     AAPDKKPPAFTADADALAAIVGGYHGAPFDILGMHPVTVAGKPGLVVRTFQPQALEVS
                     VNRNGKLYPMTRVHPDGFCELVFPGETTFFSYRLSIVLPDQSGYETEDPYRFSPVLTD
                     FDLHLFNEGNHYRLYDKLGAHLITHEGVEGVSFAVWAPNAERVSIVGEFNQWDGRRLP
                     MRARGSSGLWELFVPHLPQGTLYKYEIKTRYLGFMALKADPYAFASELRPKTASVVWD
                     LDSYQWQDEQWKSSRHLRNTLDAPISIYEVHLASWRREPDPKYGSRWLTYRELADTLV
                     PYAVEMGFTHLELLPITEYPYDGSWGYQATGYFAPTSRHGTPDDFRYFVDRAHQAGLG
                     VILDWVPGHFPKDGYALSFFDGTHLYEHEDSRLGEHQDWGTLIYNFGRNEVRAFLLSS
                     ALFWLDKYHIDGLRVDAVASMLYLDYSRQPGQWIPNQFGGRENLDAVSFLKRFNEVVH
                     AEYPDTLTSAEESTAWPMVSRPTYLGGLGFDLKWNMGWMHDMLDYAEKDPIHRRYHHN
                     TLTFSLLYAFNENFVLPFSHDEVVHGKGGMLSKMPGDYWQKFANLRTLYGYMYAHPGK
                     KLLFMGAEIGQWSEWNFDAQLDWSLLGFDHHRQLREYVAALNHVYNAEPALHQVDFTW
                     EGFQWIDVHDVDNSIVSFLRRARDPHDVVVVVVNFTPVPRTGYRVGVPVPGVWREILN
                     SDSAYWGGSNMGNAGGVPSEPTPWQGQPHSLLITVPPLAAMYFKPAQGASDKQHLQA"
     gene            6766..6969
                     /locus_tag="VFC15_01345"
     CDS             6766..6969
                     /locus_tag="VFC15_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08834.1"
                     /translation="MKCWFCDSDARGTCAACGRGLCHQHAHIHDEMTFTKTDTSTGYT
                     SYYNVYGALKCSDCRLEWKSNRP"
     gene            7012..8346
                     /locus_tag="VFC15_01350"
     CDS             7012..8346
                     /locus_tag="VFC15_01350"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HZM08835.1"
                     /translation="MIDGPSKMVASLIRSILHRVYDALWRPRRAVTHPSYARPPAPSE
                     TREPGPQPRPVAQPAIALENTELPNPWGETRLALLVVDPRRLHVYWELGPREMRKLSA
                     SPHAAVRFYDVTETPLAGAAGEHGYFEIPVELAAKNWYVDLGEPSAAYRAELGCRTSR
                     EKWISLARSNLVTMPRLHPPAIHVEPQRRAGRAVEELGAAAVAGASGHLGGGERKPAQ
                     VPAPAEAAAPVAVSTQAAEPTAAKAPRPLPDLNLPRMEPSKSAWSAAWPYAPEAAQPA
                     AVAVGVDTSVTDTGRARASLSHDKKPLATTPAEPVEHALAEDQRDSDRVWPLWVFESL
                     ATRQLVGADGIGPRDAYLHIGADPARRGPTSQPPASVAPEGTENVLRSGPLRSPGESG
                     SPGAADIAEMNEQQFVPSGAASSFPSAEPADMTDLNERQFACSGVSSSNAVR"
     gene            8352..11468
                     /gene="glgA"
                     /locus_tag="VFC15_01355"
     CDS             8352..11468
                     /gene="glgA"
                     /locus_tag="VFC15_01355"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZM08836.1"
                     /translation="METGCLAFVLHAHLPFVRHPEHDYSLEENWFYQAITETYLPLLL
                     AMDRLLEEGVDFRLTFSLTPTLASMLDDELLQSRYLARLQLLIELAEKEVVRTRSLPK
                     FHALARMYLSLLQNVQHAFQERYGRNLLTAFQRLQNVGKVEIVASCATHGYLPLLSVS
                     EAAVRTQVRVGVEHYRRIFGQQPRGFWLPECGFYTGVDQLLREQGVDYTILETHGITR
                     AVPRTHHGVYAPVACDSGLVAFGRDPESSRQVWSAVEGYPGDPDYREFYRDIGYDLDL
                     DYIGPYIHPDGIRIDTGLKYYRITGEGTDKQPYVPEWAERKAEMHAEHFLEQRIRQVL
                     GVAPHMDREPIVVAPYDAELFGHWWFEGPRWLEHVLRKAARQNTVRLVTLSEYLRDHP
                     HPPAAEPSPSSWGHKGFSEVWLNPENHWIYPQLHASADSLEKLAATQHTRSGLAERAL
                     NQATRELLLAQSSDWAFMINYGTSDLYATRRVKHHLKRFERLRNQIAAGTVEESWVAQ
                     IESQDNIFLNQDVAADFVHAAERQVPEEPVPVMPRPASAGARLRIVMVTPEAVPFAKT
                     GGLADMVSSLSLQLERAGHEVTLILPAHASALRGSVPLEELPGRLPVSMMVGAPSPAI
                     LRGKIGDAVTVYLVHAEGVFDRPQLYGTTDGDYPDNAERFVFFCRAALDVMHRLDPPH
                     ILHAHDWQAAMAVAFLKAQPWAYPKLEGTRTVFTVHNAGYQGIFDASKWPLLHLNPEF
                     FTPRYLEFYGRINFLKAGLMFADAITTVSPSYAREVQSVEQGFGLEGVFRQRAARLVG
                     ILNGADYEVWNPTNDPYVVSPYSAEDLAGKRACKADLQRNFGLPENPDVPLLGMVARL
                     VDQKGCDILANALPELFQRDVQLVVLGTGDRYYQELFRALPARYPGKAGVRIAFAEEL
                     AHKIEAGADMFLMPSRYEPGGLNQLYSLHYGTIPIVRAIGGLQDSVREFDPKSSTGTG
                     FLFESYDGAALLAAVDRSLAAFAEKTAWSSLMQNAMHADFGWAAATDRYLELYREILQ
                     EADRVSGS"
     gene            11889..15368
                     /locus_tag="VFC15_01360"
     CDS             11889..15368
                     /locus_tag="VFC15_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZM08837.1"
                     /translation="MSQQIDTRSGIKIEVDRIVSNDQVQLSLQARTPKEVVLYWTLLR
                     RPEDAWQIPPQSYWPEGTSASGTSAAQTRFAGQNGERRVTLRFDHPSDFSLMDFALYF
                     PQENRWDNNQNRNYRIALAASPAGASGASPAEVLQRELAGQEVPFQHVYDIEEGQLAV
                     AVLHADDRYRLTFVSNLPAPLVLHWGVAQRSRQEWLAPPPPVQPPGTILFQSAAETPF
                     EVVDGLNRLQLEFPQKDSPLGIPFVLRRPDVNRWIKDRGGNFFVPLAASASADTTLSD
                     GEAAQLADEIIRAETTHNSWTLMHRYNLCHDLLDRVRGNVQGLALLFVWMRFSAIRQL
                     DWQRNYNTQPRELGHAQDRLTLKLADRYVQDPAGRAFYRLILSTVGRGGDGQKIRDEV
                     LAIMHRHHIKEVTGHFMEEWHQKLHNNTTPDDVVIAEAYLEFLRSNGNHQRFYDVLQA
                     GGVTRERLESFERPIRAAPDFVGHLKEGLIHDFENFLRTLKAVHSGTDFETAINTAGY
                     LLDDDLRGRLWHLWRHQNDPPDSLVTLAYQAFEARRDVLGRMGGNQGLRELLYLDLAV
                     EQHVRTIVERSLHLHLGRDQLVDLIGALLENLVLLEAGAEFGVARTHWQRLQSLPRFT
                     AEWSLNAKAVIDRITRALGEFIDRYRAVLQPCAEVLGNGFHAAPWSITLFSEEVIRGG
                     LASTLSMLVRQLQPVLRETAHVGNWQVISRGSGTGEVQVMRSLMDGQGANYAQPTALV
                     AEAVSGEEEIPGGVVAVILGGETDILSHVAVRARDAGVLFATCYDSQVMEQLKALRGR
                     LLSFSVNSDGEVVFQEATAAASAPAPRAAISTAGLVRPRFKAYAIPASEFNERVVGGK
                     SCNQMRLHGKLPDWVRQPASVAIPFGVCEQVLELQENAPLRQRFEQLVSSVESGPPKI
                     LSQLRQTLQQLTAPSELPATLRQAMQSARLPAPEPWDDAWQCIKRVWASKWNERAWLS
                     RQAHGIRHDDLFMAVLIQQVVEADYAYVIHTVNPSTGDRGELYAEVVLGLGETLVGNY
                     PGRALSFVSSKETLQSKLIAYPSKSIGLYGGGLIFRSDSNGEDLPGYAGAGLYDSVML
                     KPPRDAELDYSQERLVWDENFREELMRCIAKIGVAVENAFGSPQDIEGAIAHGEYYVV
                     QSRDQATDAATPA"
     gene            15403..>16087
                     /locus_tag="VFC15_01365"
     CDS             15403..>16087
                     /locus_tag="VFC15_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZM08838.1"
                     /translation="MFRIGNQTAICAPTPMEPFEFALQSGFDAFEWFPDKNAAGAGWS
                     ETDLDQDARRSICNAARKHGIRLSVHAPWTANPLNPQAVPLLLQSLEFAQDVGAELLN
                     IHLYAEQGIEAYVAAIAPLMARLPEAGLALSIENTVLTPPAQFNELFARIEQLAPPGA
                     DRLGMCFDVGHANLCESTRNNYLCYLVQLNRQVPIVHVHLHENWGDRDSHLLVFTGPA
                     SRDSKGIERL"
BASE COUNT         3127 a         4872 c         4885 g         3203 t
ORIGIN      
        1 cgccatagtg atggactcga tcaggaattg ccgcagcacg tcgcgcggcc gcgctcccaa
       61 ggctttgcga atgccgattt cccgcgtgcg ctccgtcacc gacaccagca tgatgttcat
      121 gatcacgatg ccgccgacga tgagcgaaat ggaggcgatg acgatcatca cgatgaaaaa
      181 tgtgccgctc aggtcggacc agagggaaag gaaacttgcg ttggtctcga tgacaaagct
      241 gtctttttcg ccgacccggt cgtgtcttcg cgagcgcaga atcacgcggg cctgatcgat
      301 ggcttgttcg aacgacgtgc caacatcgtc ggcttttgcc cagatgttga ggctgttgtg
      361 ggccccgtag tttttgagcc atgtggtgat gggcataatc acatagttgt cgcggctctg
      421 gcccagcgtc ttgccttcct tcttgcccac gccaaccacc cggtagacct tgccatccac
      481 gcggatttct ttcccgagag gatcggtgtc ggccagtaaa ttttcgcgaa tgtcctgtcc
      541 gatgataact acggccgagg tcgtgttctc atccgcattg ttcagcatcc gacctgccac
      601 caggtcgagg tcatagatct gcgccatggt gggcgtccag ccgcgaacca tagtgtccgt
      661 gctgctctgg gtaccataga caaccttgcc attgaactgg cgtacggcag cgcccacgat
      721 cctgcacgca tcgcatccgt ccgcgaccgc ctggtagtgc tccagcgtca ggtctttgcg
      781 cttctgccct tcgatgaact ggtcgacatc ggtaatcacg ttgggtgtct tgctgatgat
      841 gaacacgtcg gtcccgagat taaagatctt ctcggcgaca tacccgttga tgccgttgac
      901 gaacgtgacc accgcaatca ccgccgccac tccgatcacc acacccagga gggtgagcac
      961 ggagcgcagt ttgttggccc aaagcgacag cagggcgata cggatggctt cagcaatatt
     1021 catgatgtgg gtatttccaa accggggcct gaaacagcta gtatctccgg tttggagtta
     1081 caagtcgcgg gaattgtaca agcgcgttcg cagcgtgcaa ttcggtaatg ccgcgtctcg
     1141 attgggaccg gcatccgggc ccgggctccg aaatctccaa ttcaagagcg gtttgtgtat
     1201 gccaaactag gagcgacttt gccgatgcac ggcgaggcgg tgtaagatcg cgtcgttttc
     1261 gaactcaaga acgaaatccc gaattcatca cgctcagagt catgagatgg tatgacccaa
     1321 ttcgaccagc cggtggcagc cgccaagcaa tggttcgcga gcccacggtt cgccggtatc
     1381 gtccgcctct actctccgca ggcacgtaga agtccaaata ggtaagcggt cacgtatgaa
     1441 ggcaaaggaa gacaagattg catcgtctcc aacatccaag gaattggtcg ttttctccat
     1501 cctgcgcgag tccagatgca aggagtgcaa caaggaactg tggaagggta attttctatt
     1561 catggaagcc gaccgcccgc tttgcttgag ctgcgctgat cttgatcatc tcacctacct
     1621 acccaggggc gatgccgcac tgacccgccg agccaagaag tacagcccgc ttttcgcagt
     1681 ggtagtccgt ttcagcaggt cacgtggccg gtacgaacgc cagggcattc taatttcgga
     1741 agccgcgctc gagcaggctg aagaagaatg ccttgcggat actgagcagc gcgcgcgacg
     1801 gcgcgaacgg gacgaggtgc gccgtgccga atatgatcgc gatctcatcg tgcacatggc
     1861 cggtgtgatc ttgaaactgt tccctgggtg tccgcccaaa gaagcgcgaa cgattgcaga
     1921 acacactgca cgccggggca gtggccgcgt ggggcgcact tccgccggcc aatcgctcga
     1981 ggagggagca cttaccgccg ctgtgattgc gtccattcgc catcgtcaca cgcgatacga
     2041 tgagcttctt atgagcggct acagccgcac cgacgcccgt ggctcgattc gcgaagacct
     2101 cgatcatgtg ctcaaaactt ggcgctatcc tccttgacct gactgagtgc cgatatagct
     2161 tccaccatca cccaagcaac ctgcgtagtc gaagctcacg agcggctagg caagagtctg
     2221 ctaatctggc aagggaatcg atgcccctgc tctttctagc cccgaccctt actccgcatg
     2281 gccgattgac tctagtacaa gacgatgacg cggcggcggt ggaagccgag cttgcacaac
     2341 ggcttcagaa cgcgttcgcc cgcggatcgg gacacggcct cccccaactg gatgcgaatg
     2401 aaatcggagt cgctcttccc ccgactttgt cctactggcg agaatttgcc gcacaagagt
     2461 cggacggatt tttccgcaag gacaagtcgc tccggagagt cggagtaagg agccgtgact
     2521 ccgctctacc atcctgacga gattccgtgg aagatgtcca ggtagcgttc tccgggaatt
     2581 ctccaggaat agtcatagcg cattccattg aggatcagcg agcggaattc ctcgggatag
     2641 ttgttccaca ggccgatggc ccgggatagc gccgattcaa tggcggccgg gtctgcctgg
     2701 tggaagacgt agccgttgcg cgcctcgccg ggtttgtcgg agtagtcgtg atcgaaaacg
     2761 gtattcacca ggccgccgac ggcacgaacc accggaacgg tgccgtactt cagcgcaatc
     2821 atctgcgtta acccgcaggg ttcgaacata ctcggtacga taaagatgtc ggatccggca
     2881 tagatgaggt gcgacagttc ctcgcggtag ccgatttcga ggtggcagtc gcggttgtcc
     2941 tgcagatagc gcttcagatt gtaaaaaagg ttgttgatgc ccggatcggg gctgctgccc
     3001 agcagcacga attgcgcgcc gtgccccagg gcatagtgca gcgcatgctg gatgaggtgt
     3061 atgcctttct gcggatcgag gcggccaaca aacgaaatca gcggcttaaa ttcctttgcc
     3121 aatccaagcc ggttgcgcaa ctcctgtttg tcggcatact tggagtccag atttccaggg
     3181 ccgtagtgaa cgggaataca ggggtcgatc tcggggttcc acacgctgta atccagacca
     3241 ttgagcacgc cggcgaattt cgtctgatga actgccaggg tggggcccat tccgaagccc
     3301 tgctccgtga agcgcgcctc ccaggcatgc tggggcgaga cggtggtgac gaagttgctg
     3361 tacacaatcc cggacttcag caagttgagg gcagagtgat tgaaatcatc ctgcatgcga
     3421 tcccggtgca tgaagaattc cggacgattg agtccgctgg ccttcagcac ttccgcgtcc
     3481 cagacgccct ggtgcttgaa attgtggatg gtgtagcaga cgcgggaatt tcccattccg
     3541 aggaatttgt aaatctcaaa gagctggaca gggacaatac cggtttgcca atcgtggcaa
     3601 tgaatgatgt cagggcggcg gtcgctcttt agcagaaact ccagtgcggc tctggagaag
     3661 aagacgaacc gcatgatgtc atcgggcgag ccatagaagt gtccgcggcg aaagaaattg
     3721 tccggcgagt gcggttcgat gaagtagcag cgcaggccat cgaccgtggc aaagaagacc
     3781 gaacaatgaa tgctcccgcc gtaccagggc acccagaggt cgtggtagac cttctccagc
     3841 ggccccatac ggtcgtaccg catgcagtcg tacaggggca ggatgatctc tacccactgc
     3901 gtgcgagtct ggagctcgcg gctcagacca ttgaccacat cacctaggcc gcccgccttg
     3961 gctgccggtg cgcattccga tgcaatctcg acgatgtaca ttggttccta tgcgtcaagc
     4021 ctgtaggtgc tgcttgtcgc tggcaccctg tgcgggctta aagtacatcg ccgccagggg
     4081 tggtaccgtg atcagcagcg aatgaggctg gccctgccac ggggtcggtt ccgacgggac
     4141 accgccggcg ttccccatgt tgctgccgcc ccagtacgcg gagtcactgt tcaaaatttc
     4201 gcgccacact cccggcaccg gtacccctac gcgatacccc gtgcgtggca cgggtgtgaa
     4261 gttcaccacc acaaccacca catcgtgcgg gtccctggcg cggcggagga agctcacgat
     4321 gctgttgtcg acgtcgtgaa catcgatcca ctggaagcct tcccaagtga agtcgacctg
     4381 gtgcagggca ggttcggcat tgtagacatg gttcagggct gccacatatt cgcgcaactg
     4441 gcgatgatga tcgaacccga gcagcgacca gtcgagttgc gcgtcgaaat tccattcact
     4501 ccactgcccg atctccgcgc ccatgaagag aagctttttg ccgggatggg cgtacatgta
     4561 gccgtagagc gtgcgcaggt tggcgaactt ctgccagtaa tcgccaggca tcttgctcag
     4621 cattccgcct ttgccgtgga ctacctcatc gtgggagaag ggaaggacga agttttcgtt
     4681 gaacgcgtac agcagcgaaa acgtaagcgt gttgtgatga tagcggcgat ggatggggtc
     4741 tttttccgcg tagtccagca tgtcgtgcat ccaccccatg ttccacttca ggtcgaaacc
     4801 caggccacca aggtaggtcg ggcgcgagac catcggccat gccgtggact cctcagccga
     4861 ggtcagggtg tcgggatatt cggcgtgcac cacttcgttg aagcgcttca ggaacgagac
     4921 cgcatccagg ttctcgcgtc caccaaactg gttgggaatc cactggcccg gctgccgcga
     4981 gtagtcgagg tagagcattg acgccaccgc atccacccgc agcccgtcaa tgtggtactt
     5041 gtcgagccag aagagcgcac tggagagcag aaaggcgcgt acttcattgc gcccgaagtt
     5101 atagattagc gtgccccagt cctggtgttc gcccagccgc gaatcttcgt gctcgtagag
     5161 atgcgtgcca tcgaagaacg aaagggcgta gccatccttg ggaaagtgcc ccggtaccca
     5221 gtccaggatg acgcccagcc ctgcctgatg cgcccggtcg acgaagtagc ggaagtcgtc
     5281 gggcgtaccg tgacgcgagg tcggtgcgaa gtatccggtg gcctgataac cccacgatcc
     5341 atcgtagggg tactcggtaa tgggcaggag ctccagatgc gtaaagccca tttccactgc
     5401 gtagggcacc agggtatcgg ccagttcgcg ataggtcagc cagcgagagc cgtacttggg
     5461 atcgggttcg cgccgccagg aagccagatg aacttcgtaa atcgagatgg gcgcatccag
     5521 ggtgttgcgc aggtggcggg aactcttcca ctgctcatcc tgccactggt agctatcgag
     5581 gtcccacacc accgacgccg tcttgggacg cagttccgag gcgaaggcgt agggatcggc
     5641 cttcaacgcc atgaatccca agtagcgcgt cttgatctca tacttgtaga gcgtgccctg
     5701 cggcagatgc ggcacgaaaa gctcccacaa gcccgatgag ccccgcgcac gcatggggag
     5761 acgtcgccca tcccattggt tgaattcgcc gacgatgctc acgcgctctg cgttgggcgc
     5821 ccacacggcg aagctcaccc cctcgacacc ttcgtgcgtg atcaggtgag cacccagctt
     5881 gtcatagagc cggtaatggt tgccctcatt gaagaggtgc aagtcgaaat cggtgagcac
     5941 gggagaaaag cggtagggat cttcggtctc gtagccgctc tgatcgggca gcacaatcga
     6001 aagccgataa gaaaagaacg ttgtttcgcc ggggaagacc agttcgcaga atccgtccgg
     6061 atgaacacgg gtcatcgggt acagcttgcc gttccggttc acggagactt ccagcgcctg
     6121 cggttggaag gtgcggacaa ccagccccgg tttccccgcc accgtgaccg gatgcatgcc
     6181 gagaatgtcg aagggagcac cgtgatatcc gccgacaatg gccgccaggg cgtcagcatc
     6241 cgcggtgaac gcgggcggct tcttgtccgg cgccgcgggc gagtctttcc tgtcagctcc
     6301 cgggggcgag gcgttgtcag ctgccgctga cgagtctttc ctatcagctt ccgcaggtgt
     6361 cactggtgcc actgcggaag gtggcgacaa gggatcgtgg gatggcatga gggcgtatcc
     6421 tttcgaaggt tgatgccaat ggccagaaag tttggttttg ggccgcttct aacgggacat
     6481 gattctaacg ggacatgatt ctaacaccgc ccctcggggc ctggtttgtg aactgggggt
     6541 cttcgaactg cgacgtggac cagagttgca tcggaaacaa tgcgcctccg tccatcatct
     6601 ccgaagccgc catctcaggg tatgatttca ggctacccca aaggaccgct gggcgtgagg
     6661 tgggcgacct gtttcgggaa tcggacgatt gttcaccgcc agacgtttcg cctcgtggtt
     6721 gagggatgta gtaagatcga tttttgcaag gaaactggtc gaatcatgaa atgttggttc
     6781 tgcgactctg acgcacgcgg cacctgcgcg gcttgcggac gcggattgtg ccatcagcat
     6841 gcccacattc acgatgagat gacgttcacc aagaccgata ccagtacggg ttacaccagc
     6901 tattacaacg tctatggagc gctgaagtgc tcggattgcc gcctcgagtg gaagagcaat
     6961 cgcccctgag cctgataccg gcgtttcgag ccccgagccc cgctggaaac tatgattgac
     7021 ggcccctcga agatggtcgc gtcgttaatc cgcagcattc tgcatcgcgt ctacgacgcc
     7081 ctctggcgtc ccaggagagc agtgacgcac cccagttacg cgcggcctcc cgctcccagt
     7141 gaaacacggg agcctggtcc tcagcctcgt ccggtggcgc aaccggcgat tgctttggag
     7201 aatacggaat tgcccaaccc ctggggcgaa acccggctgg cgctgctggt cgtggatccc
     7261 cgcaggctgc atgtttattg ggagcttggg ccgcgagaga tgcggaagct ttccgcttcg
     7321 ccgcacgcag cggtgcgttt ctatgacgtt accgaaacgc cgcttgcagg ggctgctggg
     7381 gagcacggct attttgaaat ccccgtcgaa ctggccgcca agaattggta cgtcgacctg
     7441 ggggagcctt cagccgcata ccgcgccgaa ctcggatgcc gcacgtccag agaaaaatgg
     7501 atctcgctgg ctcgctccaa cctcgtgacc atgccgcgcc tgcaccctcc cgcgatacac
     7561 gttgagccgc agcgccgtgc tgggagagcg gtggaggagc ttggcgccgc agcagttgcg
     7621 ggggcaagtg ggcatcttgg tggaggagag cggaaacccg cgcaggtgcc ggctcccgct
     7681 gaggcagccg ctccagtcgc ggtttcgacg caagcggcgg agccgactgc ggcgaaggcc
     7741 ccgcgccctc ttcccgatct gaacttgccg cgcatggagc catcgaagtc tgcttggtca
     7801 gcggcgtggc cgtatgctcc ggaagcggcg caaccggcgg ccgttgcagt cggagtcgat
     7861 acgtcggtca cggacacagg gagggcgagg gcttccctga gccacgataa aaagccgctt
     7921 gcgacgactc ctgcggagcc ggtcgagcat gcattggcag aggaccagag ggattcggat
     7981 cgcgtgtggc ccttgtgggt tttcgaaagc cttgcgaccc ggcaactggt aggcgcggat
     8041 ggaattgggc cccgggatgc gtacctccac atcggcgcag atcccgcccg cagggggcca
     8101 acctcgcagc cgccggcttc cgttgctccc gaaggaacgg aaaatgtgct tcgatccggg
     8161 ccgctacgct caccaggcga atccggttcg ccgggagcgg ctgacatcgc cgagatgaac
     8221 gaacagcagt ttgttccgtc aggagcagct tcctccttcc cctcggctga acccgctgac
     8281 atgaccgatc tcaacgaacg gcagtttgct tgttcgggag tttcttcttc gaacgctgta
     8341 cgctgaacgc aatggaaaca ggctgcctgg ctttcgtgct ccacgcccat cttcccttcg
     8401 tgcgtcaccc cgagcacgac tactcgctcg aagagaactg gttctaccag gccattacag
     8461 aaacctatct gccgctgctg ctggctatgg atcgcctgct ggaggagggc gtcgacttcc
     8521 ggctgacgtt ctcgctgact cccactctgg cgtccatgct cgatgacgag ctgctgcagt
     8581 cccgctacct ggcccgtctc cagcttctta tcgaactcgc cgagaaagaa gttgtgcgca
     8641 cccgctcact gcccaagttt catgccctgg cgcgcatgta tctctcgctg ttgcaaaacg
     8701 tgcaacatgc gtttcaggag cgctatggtc gcaacctgct caccgccttt cagcggttgc
     8761 agaacgtggg caaggtcgag atcgtcgcca gttgcgccac gcacggctat cttccgctgc
     8821 tctccgtcag tgaggctgcg gtgcgcacgc aggttcgcgt gggtgtcgaa cattaccgca
     8881 ggatttttgg gcagcagccc cgcggattct ggctgccgga atgcggcttc tacacgggcg
     8941 tcgaccagct tttgcgcgag cagggcgtgg actataccat cctcgaaacc cacggcatca
     9001 cgcgcgccgt gcctcgcacc caccacgggg tttacgctcc cgtggcctgc gactctggac
     9061 tggtcgcctt cgggcgcgat cccgaatctt cccggcaggt gtggagtgcg gtggaaggct
     9121 accccggcga tcccgattat cgcgagttct accgcgacat cggctatgat ctcgacctcg
     9181 actacatcgg tccttacatt catcccgatg gcatccgtat cgacaccggc ctgaagtact
     9241 accgcatcac cggcgaaggt accgacaagc agccctacgt tcccgagtgg gccgagcgca
     9301 aggccgagat gcacgccgaa cattttctgg aacagcgcat tcgccaggtg ctcggtgtcg
     9361 ctccgcacat ggatcgcgag cccattgtgg ttgcgcctta cgatgccgag ctcttcggcc
     9421 actggtggtt cgagggaccg cgctggctgg agcacgtgct gcgcaaggcc gcgcggcaga
     9481 acacggttcg gctggtcacc ctctccgagt acctgcgcga tcatccgcat ccgcccgccg
     9541 ccgagccctc accctcgagc tggggacaca aaggtttcag cgaagtgtgg ctcaatccgg
     9601 agaaccactg gatctacccg caactgcacg cctctgccga tagcctggag aagctggcgg
     9661 ccacgcagca cacacgttcg gggctcgcgg aacgggcgct gaatcaggct actcgggagt
     9721 tgctgctggc ccagtccagt gactgggcct tcatgatcaa ctacggcacc agcgatctgt
     9781 atgccacgcg ccgcgtgaag catcacttga agcgcttcga gcgcctgcgc aaccagattg
     9841 ccgccggcac cgtcgaggag agctgggtcg cgcagatcga gagccaggac aacatcttcc
     9901 tgaaccagga tgtcgcggct gattttgtgc acgcggcgga acggcaggtg ccggaggagc
     9961 ccgttcccgt tatgccacgt cctgcttcgg caggcgcgcg cctgcgcatc gtgatggtca
    10021 cgccggaagc cgtgcccttc gccaagaccg gcggactggc cgacatggtg agttcgttgt
    10081 cgctgcaact ggagcgggca gggcatgagg tcacgctgat cctgccggcg catgcttccg
    10141 ctttgcgcgg ctcggtgccg ctggaggaac tgccggggcg tctaccggtc tccatgatgg
    10201 ttggcgctcc cagtccggca attctgaggg gcaaaattgg cgacgcggtc acggtgtatc
    10261 tggtgcacgc cgagggcgtg ttcgaccggc cgcagctcta cggaacaacc gacggcgact
    10321 accctgacaa cgccgagcgt ttcgtcttct tctgccgtgc cgctctggac gtgatgcacc
    10381 gtctcgatcc gccgcacatt ctgcacgcgc atgactggca ggccgccatg gcggttgcgt
    10441 tcctgaaggc gcagccctgg gcttatccga agctcgaagg gacgcggacg gtcttcaccg
    10501 ttcacaacgc cggataccag ggaatcttcg acgcctccaa gtggcctctg ttgcatctca
    10561 accccgagtt cttcactccc cgttatcttg agttctacgg gcgtatcaac ttcctcaagg
    10621 ccggactgat gttcgccgat gccatcacca ccgtcagccc aagctacgcc cgcgaggtgc
    10681 agagcgtgga gcagggcttc ggcctcgagg gcgtcttccg gcagagggcg gcgcgcctgg
    10741 tgggaatcct gaacggcgcc gactacgaag tctggaatcc caccaacgac ccctacgtgg
    10801 tgagccctta cagcgccgag gacctcgcgg gaaagcgcgc ttgcaaggcc gatttgcagc
    10861 gcaacttcgg cctgcccgag aacccggatg taccgttgct cggcatggtg gcgcgtctgg
    10921 tcgatcagaa gggttgcgat attctggcga acgctctgcc ggagctgttc cagcgggacg
    10981 tccaactcgt cgttctcggc actggggatc gctattatca ggagctattt cgcgccttgc
    11041 cggcacgcta tccgggcaag gccggtgtcc gcattgcctt cgcggaggaa ctggcccaca
    11101 agatcgaggc gggcgccgac atgtttctca tgccctcgcg ctacgagcct ggtggattga
    11161 atcaactcta cagcttgcac tacggcacca tcccaatcgt tcgcgccatt ggcggcttgc
    11221 aggacagcgt acgcgaattc gatcccaagt ccagcactgg caccgggttc ctcttcgagt
    11281 cgtacgatgg cgcagcgctg cttgcggcag tcgatcgctc gctggccgcc ttcgcggaga
    11341 agacggcgtg gagttcgttg atgcagaacg cgatgcacgc cgacttcggc tgggcggccg
    11401 ccacggatcg ctacttggag ctctatcgcg agattctgca ggaggcggat cgggtttccg
    11461 gatcgtgatc cgcaaacccg cgccagcggc cgcccgctcg cagagtttct cttcaacgaa
    11521 agtggagtga acagcgaaga ccgacaggag catcccaggc aattcccaaa tccggcctat
    11581 ctcaaccgat tgcccagttg ccacaatgct tgttgcccac aggtcccaat tcttatcgta
    11641 gaatttggca tctaatgaca aagggaaatg cgccgtcgat acgaaagtga tactttctgc
    11701 acgtttatcc aactggcaga ccagttgcct gtttcatcgg ccaatcgagc gcttcaatga
    11761 agcagaggcg gcatgacggg ccatcgctcc gaagcggtgg cagcgcgttt gccgcaccgc
    11821 tgacaggcca gagggctgcg catcgattgc ggtagcgaaa gcgttttgat cgaactctta
    11881 atcgaactat gagccaacag atagacaccc ggagcggcat caagatcgag gttgatcgga
    11941 tcgtcagcaa cgatcaggtg caattgtcgt tgcaggcgcg gactcccaag gaagtggtgc
    12001 tgtactggac cctgctgcgc aggcccgagg acgcgtggca gattccaccg cagtcctatt
    12061 ggcctgaagg cacttcggct tcgggcacgt cggccgcgca gacccgcttt gccggacaga
    12121 atggcgaacg ccgcgttacc ttgcggttcg atcatccgtc cgatttttct ctgatggact
    12181 tcgccttgta tttcccgcaa gagaatcgct gggataacaa ccagaaccgt aactaccgca
    12241 tagcgctggc cgcatcgccc gccggggcat ccggcgcgtc cccagcggaa gtgttgcagc
    12301 gcgagcttgc cggccaggaa gtcccgttcc agcacgtcta cgacatcgaa gagggccagc
    12361 ttgccgtagc cgttctgcat gctgatgacc gttaccggct gacgttcgtc tccaatcttc
    12421 ccgcgcctct ggtgctgcat tggggcgtcg cgcagcgttc gcgccaggaa tggctcgcgc
    12481 cgccgccgcc ggtacagccg cccggtacca ttctgtttca gtcggctgca gagacaccgt
    12541 tcgaggtagt tgacggcttg aaccggctgc agttggaatt tccccaaaag gactcgccgc
    12601 taggaattcc gttcgtcttg cggcggcccg acgtcaatcg gtggatcaag gatcgcggcg
    12661 gcaacttctt cgtcccgctg gcggcgtccg cttccgcaga taccacattg tcagatgggg
    12721 aagcggccca gcttgccgac gaaatcatcc gggctgagac cacgcacaac tcctggacgc
    12781 tgatgcatcg ctacaacctg tgtcacgacc tgctcgatcg cgtccgcggg aacgtgcagg
    12841 ggctggcgct gctcttcgtc tggatgcgct tctcggccat tcgccagctc gactggcagc
    12901 gcaactacaa cacccagcca cgggaattgg gtcacgctca ggacaggctc acgctcaagc
    12961 tcgccgaccg gtacgtgcaa gatcccgccg gccgggcatt ctatcgcctt atcctctcca
    13021 cggttggccg cggcggcgat ggtcagaaga ttcgcgacga agtcctggcc atcatgcacc
    13081 gtcatcacat caaggaagtc accggccact tcatggaaga gtggcaccag aagctgcaca
    13141 acaatacgac tcccgacgac gtggtcatcg ccgaagcgta tctcgaattc ctgcgcagca
    13201 acggcaatca ccagcgattc tatgatgtgc tccaggcagg cggggtcacg cgtgagcgac
    13261 tggagagctt cgagcggccc atccgcgccg cgcccgactt cgtgggccac cttaaagaag
    13321 gacttatcca cgacttcgag aatttcctgc gaaccttgaa ggccgtgcac tccgggacgg
    13381 acttcgagac cgcgatcaac accgctggct acctgcttga cgatgatctg cgcggccgcc
    13441 tgtggcatct gtggcggcat cagaacgatc cgccggattc gctagtgacc ctcgcctatc
    13501 aggcgttcga agcccgccgt gacgttttgg gtcgcatggg aggcaatcag gggcttcgcg
    13561 aacttttgta ccttgatctt gcggtggagc agcatgtgcg caccatcgtg gagcgcagcc
    13621 tgcacctgca tcttggccgc gatcagttgg tcgatctcat cggcgcgctg ctcgaaaacc
    13681 tggtacttct cgaagccggc gcggagttcg gggtggcgcg cacgcattgg cagcggttgc
    13741 agagcttgcc gcgcttcact gccgagtggt cgctgaacgc caaggcggtc atcgatcgca
    13801 tcacgcgcgc gctcggcgaa tttatcgacc gctaccgcgc tgtgctgcag ccctgcgccg
    13861 aagtgctggg caatggtttc cacgccgctc cctggagcat cacgctcttc agtgaagagg
    13921 taattcgcgg cggcctggcc tcgacactgt cgatgctggt tcgccaactg caacctgtcc
    13981 tgcgcgagac ggcgcatgtc ggcaactggc aggtcatcag ccgcggcagc ggaactggcg
    14041 aggttcaagt catgcgctcc ctgatggatg gtcagggggc aaactacgcc cagccgacgg
    14101 cgctggttgc cgaggccgtg tcgggcgaag aggaaattcc tgggggagtt gtcgcggtga
    14161 tcctcggagg tgagacggac attctgtcgc acgtcgcggt gcgcgcccgc gatgccggcg
    14221 tgctgttcgc cacctgctac gactcgcaag tgatggagca gctcaaagcg cttcgtgggc
    14281 gtctgctcag cttttccgtc aactccgacg gcgaggtcgt cttccaggaa gccactgcgg
    14341 cggcgagcgc gcccgcgcct cgcgctgcca tctctactgc gggactcgtt cgccccagat
    14401 tcaaggccta cgcaattcca gctagcgaat tcaacgagcg cgtggttggc ggcaagtcgt
    14461 gcaaccagat gcgtttgcac ggcaagttgc cggactgggt tcgtcagccg gcttcggtgg
    14521 caatcccgtt cggggtttgt gagcaggttc ttgagttgca ggagaacgcg ccgctgcggc
    14581 agcgcttcga acagctcgtt tcctcggtcg agagcggtcc tccgaaaatc ctctcgcagc
    14641 ttcgccagac gttgcagcaa ctgactgcgc cgagcgaatt gccggcgacc ttgcggcagg
    14701 cgatgcaaag cgcgagacta cctgctccag agccctggga cgacgcatgg cagtgcatca
    14761 agcgggtgtg ggcctcgaag tggaatgagc gcgcgtggct cagccgccag gcccacggca
    14821 tccgccatga cgatctgttc atggccgttc tgatccagca ggtggtggag gccgactatg
    14881 cctatgttat ccacaccgtt aatccctcca cgggcgaccg tggcgagttg tatgccgaag
    14941 tggtgctggg gctcggcgaa accctggtcg gcaactatcc cggacgggcg ctcagcttcg
    15001 taagtagtaa agaaactctg caatcgaagc tgatcgcgta tcccagcaag agcatcggtc
    15061 tctatggcgg aggtctcatc ttccgctccg attccaatgg tgaagatctg cctggctatg
    15121 ccggagccgg tctctatgac agcgtgatgc tcaagccgcc acgcgatgct gagctcgact
    15181 actcgcagga gcgcctggtg tgggatgaga attttcgcga ggaactgatg cgctgcattg
    15241 cgaagatcgg cgtggccgtg gagaatgcct tcggttctcc gcaggacatc gagggcgcca
    15301 ttgcgcacgg cgagtactac gtggttcagt cgcgtgacca ggccacggat gccgctacgc
    15361 ccgcgtaacc actgagcgat ttcggaaacg aggaaaccag acttgtttcg catcgggaat
    15421 cagaccgcga tctgcgcgcc cacgccgatg gagccctttg aattcgcgct gcaaagcggc
    15481 ttcgatgctt tcgagtggtt tcctgataag aatgccgccg gcgcgggctg gagcgagacc
    15541 gatctcgacc aggacgcgcg gcgctccatt tgcaacgccg cgcgcaagca cggcattcga
    15601 ctatcggtgc acgcgccgtg gacggccaat cccctcaatc cccaggccgt gccgcttctg
    15661 ctgcaaagcc tcgagttcgc ccaggatgtg ggcgccgagc ttctcaacat ccatctttat
    15721 gccgagcagg gaatcgaggc ctacgtcgct gctatcgcgc cgctgatggc ccgattgccc
    15781 gaagccggat tggcgctctc gatcgaaaat acggtcctca caccgcccgc gcaatttaac
    15841 gaactcttcg cgcggatcga gcaactcgct ccacccgggg ctgatcgtct tggcatgtgc
    15901 ttcgatgtgg gacatgcgaa tctttgcgag tccacgcgca acaattacct gtgctacttg
    15961 gttcaactca accggcaggt gcccatcgtg catgttcacc tgcacgaaaa ctggggcgat
    16021 cgcgacagtc atctgctcgt cttcacggga ccggcgagcc gagacagcaa ggggatcgaa
    16081 cgcttgc
//
LOCUS       DASRKK010000069         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_394758, whole genome shotgun sequence.
ACCESSION   DASRKK010000069 DASRKK010000000
VERSION     DASRKK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394758"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFC15_01370"
     CDS             complement(<1..902)
                     /locus_tag="VFC15_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM08839.1"
                     /translation="MLRRSQCRRGVLVWLVLLPLYGAAQTLPQFSPTPSATPLRLTLK
                     AAVQLALKQNPQRIAAHLITLQSERDRQIARAPLLPQAGLVANDSLNQYNFQSVEKSK
                     RQPAGPYQYVEAGPAFSQTIFDLPQIRGYQIGKEGVREARAEESVTRETVSLAIVSQY
                     LLVLRAQAKYEAAKARVALAERLFQQAEELQKTGVGLKIDTLRANVELQNEKQGLIDA
                     QTATRTTTYVLAELLDLPPDQEPEVADRMEFFALPAYDRPTMLNQALANRPEISSIAS
                     QQRIASLAHKSASEQRLPQLDFSG"
     gene            1205..1450
                     /locus_tag="VFC15_01375"
     CDS             1205..1450
                     /locus_tag="VFC15_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08840.1"
                     /translation="MMYFEIVGEIKQVEVIATALGVRDRKRLWRAYGRGSWRKLKGIA
                     SIKLRDGALVIAELPWYEAHGIGKKEFKIKRLVAQEG"
     gene            1473..1667
                     /locus_tag="VFC15_01380"
     CDS             1473..1667
                     /locus_tag="VFC15_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08841.1"
                     /translation="MRNEGYPASLERRKLYPIVQDASAAKHNLVRIIDESGGDYLYPE
                     DYFVALRLPPKVKLALSRAS"
     gene            complement(1849..2550)
                     /locus_tag="VFC15_01385"
     CDS             complement(1849..2550)
                     /locus_tag="VFC15_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZM08842.1"
                     /translation="MSKLVSDQAPSRRQFVQMGLAGAAVGLLTGGMELAFPPPASAQS
                     TLSPDQALNELMAGNKRFTSGQLTSFEHDLAILKQHTVDKQEPFAAVLSCADSRVPVE
                     LVFDQSIGHIFVTRVAGNVITPEIIGSLEYGAAVLGTKVILVMGHSGCGAVKATIQGK
                     EVPGQISSLFPHIQPGVDRGGPNLEAAAKANAQIQATLLREASTVIAQLTKDAKVKVI
                     AGYYDLGSGAVELLT"
     gene            complement(2813..>4560)
                     /locus_tag="VFC15_01390"
     CDS             complement(2813..>4560)
                     /locus_tag="VFC15_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM08843.1"
                     /translation="SPLSAAAANKSKTAAADANEPSYDLPQPQRETLDYGMYQRIRDE
                     GLGHSHAMEYASALDDDIGPRLTGSANLKHANEWTRDQFTAMGCSNAHLEDWGEFGMG
                     WQQLNTWIRMTAPDTAVFIAQAAPWSPATNGAINAPAIWIDVKDAKDLDQYKGKLAGK
                     IVLYGEMRDVPPVDKPLFVRLDDAELKKLTEYPLKPRGRDFIEQYMKRLELRQKVGQF
                     LADEHALAVVTPSRDGRDGGGSGGTVFDDSGAGFGWEAYKREHANPLPVVVTAIENYG
                     RVYRLLKAHVPVTMEMNIDTKFTGDHEHGYDTIAEIPGTDPKLKDEVVMVGGHLDSWA
                     SGTGATDNGAGTVVSMEVMRILNTLQVKPRRTIRVALWTGEEEGEFGSYGYVKQHFGN
                     VPLSTAPDQLKLPEWVRKPAGPVQLKPDQQKISGYFNVDNGSGKIRGIYLQENAAIAP
                     IFAQWIEPLKDLGVTTVTMRNTGGTDHEAFDAVGIPGFQFIQDPLDYGSRTHHSNMDT
                     YERLQPEDLAQAAVVEAIFVYNTAMRDQMLPRKPLPHPELEEQRRAPLTNIMPGAEAA
                     EKDAKEKADQTPKEE"
BASE COUNT          895 a         1325 c         1321 g         1019 t
ORIGIN      
        1 aagccggaaa agtccagttg gggcaggcgc tgctcggagg cggacttatg ggcgaggctc
       61 gcgatacgct gctgcgacgc gatcgaactg atctcgggac gattggcgag agcctggttc
      121 agcatggttg ggcgatcata ggcaggcagc gcgaaaaatt ccatgcgatc ggcgacttcg
      181 ggctcctggt cggggggaag atcgagcaac tcggccagga cgtaagtcgt agtccgcgtc
      241 gccgtctgcg cgtcgatcaa accctgcttc tcgttctgta attctacgtt ggcgcgcaaa
      301 gtgtcgatct tcaagccgac gccggtcttc tgcaactcct cggcctgctg gaaaagccgc
      361 tcggccagcg ccacgcgagc tttggccgcc tcatactttg cctgcgcacg aaggaccagc
      421 agatactggc tcactatggc aagcgacacc gtctcgcgag tgacgctctc ctcggctcgc
      481 gcttcccgca ccccctcctt gccgatctgg taaccgcgga tttgcggcag gtcgaagatg
      541 gtttgggaaa atgcaggtcc ggcttcgaca tattggtaag ggccggcggg ctgacgcttg
      601 gacttttcca cactctgaaa gttgtattgg ttgagcgaat cgttggccac gagcccggcc
      661 tggggcaaca acggcgcacg ggcgatctgg cggtcgcgct cgctctgcaa ggtgatcagg
      721 tgggctgcga tgcgctgcgg gttttgcttc aacgcaagtt ggaccgcggc tttcagcgtc
      781 aggcgcagcg gtgttgccga aggggtgggg gaaaactgcg gaagggtttg cgccgcacca
      841 tacaaaggaa ggaggaccag ccaaaccagt acgcctctac gacattgcga tcgccttagc
      901 attgttaaac ccggctttgc cgttggttgc cgcgatttcg cgttggcaac aggaatagca
      961 cggcgttctc tcccccggac ataaggtgta aggaatttta gtcagaaacc aacgatatgc
     1021 gaaatctggg gagatgccgt ctgcaaaaag gccggcactg ttctgtggga gccgccaatt
     1081 cgcgctttct tacgaatgag tgaagaccac tatcctgctg aggatgaaga gctagggatg
     1141 gacgagacaa gcatcccacc caaccaagga cgggttgtgg caccctcccc cggttacaat
     1201 ctctgtgatg tattttgaaa tcgtcggcga gatcaagcag gttgaggtga tcgcgacggc
     1261 tctgggagtt cgcgaccgca aacgcttgtg gagagcctat ggccgcggct cctggcgcaa
     1321 gttgaaaggt atcgccagca tcaaacttag agatggcgca ctggtgatcg ccgagttacc
     1381 ttggtatgaa gcccatggta tcggtaagaa ggaattcaaa atcaaacggc tcgtcgcgca
     1441 agaaggctaa aggcgaggcc ttcgtccttt gcgtgcgtaa tgaaggctac ccggcatctc
     1501 tggaacggag gaagctgtat cccatcgttc aggatgcgtc cgcagcgaag cataacctgg
     1561 tgcgcatcat cgacgaatct ggcggggact acctctatcc ggaagattac ttcgtcgcgc
     1621 tccgcctgcc accaaaagta aagctcgctc tatcgagagc atcttagttc caatccaacg
     1681 gagggaacca cccactgggc catgatcgga aagaacccga gtcccttcag gggctgcata
     1741 tgtggcatag ttcccaccga aagtcgcgag tcatgagctg ccccacccaa gcaaaagcgg
     1801 cttgagttga caacgccttc tctctgatcg tgggcagcct gtacgcggtt acgtgagcag
     1861 ctctacggcg ccactaccca ggtcgtagta acctgcgatc acttttactt ttgcatcttt
     1921 cgtgagttgc gcaatcacgg tcgatgcctc gcgcaacagg gtcgcctgga tctgggcatt
     1981 cgctttggcc gccgcttcca gattcggtcc accccgatcc acgcccggct ggatgtgagg
     2041 aaacagagag ctgatctgac cgggtacctc tttgccttgg atcgtggctt ttaccgctcc
     2101 gcagccggag tgtcccatca caagaatgac tttcgtgccc agaaccgcgg ctccgtattc
     2161 gaggctgccg atgatctccg gcgtgatcac atttcccgcc actcgggtca cgaaaatatg
     2221 tccaatactc tgatcgaaaa ccaactccac cggtactcgc gagtccgcgc aggacagtac
     2281 cgcggcgaat ggctcttgtt tgtcgaccgt gtgttgcttg aggatagcca ggtcatgctc
     2341 aaaggaagtt agctgccctg aagtaaaccg cttattgccc gccatcagct cgttcaatgc
     2401 ctggtcaggg ctgagcgtac tttgcgccga cgctggtggc ggaaaggcaa gttccatgcc
     2461 gccggtgagc aagcccacag cagccccagc caaacccatc tgcacgaatt gtcgtcttga
     2521 cggcgcctga tccgaaacaa gtttgctcat atgactcctt cacttaagta ttttgcacaa
     2581 gggttcgcgc tcacaatcgc tgtttccagc cgtttcaaag acgactttgc aaatactctt
     2641 ccgacgcggc gcaattgtgc gaccgacatt atgcaacgga ttccggagtg gcaacaacca
     2701 aaaaacgcac gcagcgcgcg gctgtgatct cccactgttt cacaccattt tgcggaaaag
     2761 ctgtaaaggc ccccaccaag ctgctcttgc ttgggcggga cacttcgccc tcctactctt
     2821 ccttcggcgt ttggtctgct ttttcttttg cgtccttctc cgcggcttcg gctccgggca
     2881 tgatgttggt cagtggcgcc ctacgctgct cttccagttc agggtgcggc aacggcttgc
     2941 gcggcagcat ctggtcgcgc atggcggtgt tgtacacgaa aatggcttcc accaccgctg
     3001 cctgggcaag gtcctctggt tggagacgtt cgtaggtgtc catgttcgag tggtgagtgc
     3061 gcgaaccata atcgagtggg tcttgaatga actggaaccc gggtatgccg acagcgtcga
     3121 acgcttcgtg gtcggtacct ccggtattgc gcatcgtcac tgtagtgacc ccaaggtctt
     3181 tgagcggctc aatccactgg gcaaagatcg gcgcgattgc cgcgttctcc tgcaagtaaa
     3241 tgccgcggat ctttccgctg ccgttgtcca cgttgaaata gccggagatc ttctgctgat
     3301 cgggcttgag ctgtaccggg ccagcgggct tgcgtaccca ctccggcagc ttgagctgat
     3361 ctggtgcagt ggatagcggc acattgccga aatgctgttt tacgtaaccg taggaaccga
     3421 actcgccttc ttcctcgccg gtccacaagg ccacgcggat ggtgcggcgt ggcttcacct
     3481 ggagcgtgtt gaggatgcgc atcacttcca tggagacaac cgtgcctgca ccattgtcgg
     3541 ttgcaccggt gccagaagcc cacgaatcga gatgtccgcc aaccatcaca acttcgtcct
     3601 tcagtttcgg atcggtgccg gggatttcgg cgatggtgtc gtacccgtgc tcatggtcgc
     3661 cggtaaactt agtgtcaata ttcatttcca tcgtgaccgg aacatgcgcc ttcagcagcc
     3721 ggtatacgcg cccataattc tcaatggccg tgaccacaac gggaagcggg ttcgcgtgct
     3781 cccgtttgta cgcctcccag ccaaaaccgg cgccactgtc gtcgaaaacg gtgcccccag
     3841 agcctccgcc atcacgaccg tcgcggcttg gggtaacgac cgcaagagcg tgctcgtccg
     3901 ctagaaactg tcccaccttc tgccgtaact ccagccgctt catgtactgc tcgatgaagt
     3961 cccggccgcg cggcttgagt gggtactcgg taagcttttt cagctccgcg tcatctagtc
     4021 gcacgaagag tggtttgtcg accggaggca catcccgcat ctcgccatac agcacgatct
     4081 ttccggccag tttgcccttg tactggtcga ggtctttcgc gtcttttaca tcgatccaga
     4141 ttgctggcgc attgatcgcg ccattcgttg ccggagacca cggggctgcc tgtgcgatga
     4201 acaccgcagt gtcgggcgcc gtcatgcgta tccaggtgtt gagttgttgc catcccatgc
     4261 cgaactcgcc ccagtcctcg aggtgggcat tgctgcaccc catcgcggtg aactgatcgc
     4321 gcgtccactc attggcatgt ttcaggttgg ccgagccggt caagcgcggt ccgatgtcgt
     4381 cgtccagcgc ggaggcatac tccatcgcat gggagtggcc gagtccttcg tcccgaattc
     4441 gttggtacat gccgtaatcc agagtctcgc gctgcggttg tggcaggtca taggatggct
     4501 cgttcgcatc ggcggccgct gtttttgatt tgttggctgc cgcagcggag agaggactgt
//
LOCUS       DASRKK010000070        19319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1079031, whole genome shotgun sequence.
ACCESSION   DASRKK010000070 DASRKK010000000
VERSION     DASRKK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19319
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079031"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFC15_01395"
     CDS             <1..264
                     /locus_tag="VFC15_01395"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HZM08844.1"
                     /translation="KQHSDPRNRILHAIGTTLGLTVVSGALILGHPWYALLFFPIGYG
                     FAWTGHFVLEKNKPASFGHPFWSFISDFRMLGLMFTGRLKARM"
     gene            328..831
                     /gene="ruvC"
                     /locus_tag="VFC15_01400"
     CDS             328..831
                     /gene="ruvC"
                     /locus_tag="VFC15_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZM08845.1"
                     /translation="MRVLGIDCGGEYTGYGVVEQDDQGVLLHVCSGAIRLLPREALEI
                     RLMKICRGLTEVIGSFSPEVVTIEDVFYAVNAKSALKLGHVRGVAMLTAAQAGLSVIA
                     YSPLSIKSAVVGYGRAEKSQVQLMVARLLNLPEPPEPPDVADALAIAICHLHTAATLR
                     RQHATTK"
     gene            1001..1708
                     /locus_tag="VFC15_01405"
     CDS             1001..1708
                     /locus_tag="VFC15_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08846.1"
                     /translation="MVVPEERRIEKDGRVIGMSRNLMGGLLCLIDAQGASVSLERLKG
                     AMWPSSIVSDETARSYMDTLQRALETDSAPGSVVESNPECGYRLLVNARPAGPDDAPE
                     SELTRQQVPADPFEVNADVAGYILSYHSNLMTELEHRAQGHFHAKMKMTGWGDRSPQL
                     QASVSQEYSRYLATDPAVLALVNDGYEAFAERAANRILKEYGDRLNLNRCPSCACIAR
                     TPKARQCRFCGRDWHDG"
     gene            1843..2574
                     /locus_tag="VFC15_01410"
     CDS             1843..2574
                     /locus_tag="VFC15_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08847.1"
                     /translation="MLRLKYVLTFWLVCALSATAQVVHQVDPAETPTGSYVTFKFDWD
                     QGRPWLRYSIAVDDAGNAHFEGVGNPIESGDSDAFTEDFTMTDINRRKIFDLAKKLDY
                     FQGNFEAKQKNIAKTGQKTLGYHGHPAGGGQTIEHSSTYNYSPNSDVEELTRVFQAIA
                     TTLDYGRKLAFKFRYDKLGLDAELKDLQDMQASHYIEELQAIEPILQKIADDPNMMHI
                     NRVTARQMLKAMGTTGVPSQTTSEP"
     gene            2790..4298
                     /locus_tag="VFC15_01415"
     CDS             2790..4298
                     /locus_tag="VFC15_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZM08848.1"
                     /translation="MAKRQKCGLAVMAAMLLAVVSAQATSHVRIVRLSYEDGNVQMSR
                     GTGQGLERAILNSPIVEGSSVVTGRDGLAEVEFENNSTVRLGEATDMSFRQLLINDAG
                     EKVNEVELVRGTMYFDTRSGKDNVYRVVATDRTFVVQRNSLVRFMMSGDQVEVAVMNG
                     TAQLANSAEPVKLKKNETLTSDSTNPQGFTMAKGVDSTPVDRWNSERAAYQTVYAYNN
                     TGYGSYGSPGLGSYGFQDLAYYGGFLNLPGYGMVWQPYGASNWLGWNPYLCGAWAFTP
                     GFGYAWASAYPWGWLPYHYGAWGYSSGIGWFWAPGNTMGHGGIATNWQPTAPVIHGPP
                     GYTAPLPPTTTSVGSQSTVLVGRIARTPAYIPGGPVPPDFRSVIGRSSMIGTTGTATG
                     ATGAAGSVARNSKAASLIGSTTTAPAGNTFATPGANSSTAGSSHSTSGSSYNRSGHVF
                     VVPTAPVWTSQGYWTPGGGSATSGQASWAPAHSSGGTSRATGTGGAAHSTPK"
     gene            complement(4366..5202)
                     /gene="amrB"
                     /locus_tag="VFC15_01420"
     CDS             complement(4366..5202)
                     /gene="amrB"
                     /locus_tag="VFC15_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZM08849.1"
                     /translation="MITAIRQPAVAGRFYPGNAQHLRTEVETFTTPRAVAGQVAIEIK
                     LHAVGCVVPHAGYIYSGAVAGAVYRRLILPRRCVILCPNHTGMGEPLAIMSQGAWHTP
                     LGDVPVDAELADALKSVVPLLTEDPAAHRYEHALEVQLPFLQVLRPGFQFVPITVGTS
                     NFEDLSELGDAIGSLLSGLSEPALVIASSDMNHYESDSVTRVKDRRAIDQVLALDPRG
                     LYDTVRQGNISMCGYGPATIMLTAVRKMGATQAELIRYATSGDVSEDLDMVVGYAGIA
                     VY"
     gene            complement(5265..6134)
                     /gene="rsmA"
                     /locus_tag="VFC15_01425"
     CDS             complement(5265..6134)
                     /gene="rsmA"
                     /locus_tag="VFC15_01425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZM08850.1"
                     /translation="MVKSASHEKEVNTTGGKRKSKLGQNFLIDQSAARRIVDALGDVS
                     SRTVVEIGPGRGMLTDQLVKRARRVIGIELDHVLAAQMRMRYATLQNVEILESDFVTV
                     EWISMVGRRPGPLHDLRPTQPETVDIIGNLPYYVTSDIVLRILEEHRNIGRAVIMVQR
                     EVADRISAEPGSRDYGLLSATAQLFARVDNLFTLPPGAFDPPPKVHSSVLRLTMAPRL
                     AELQIEAGPFLDMLKLAFAQKRKTLVNNLKGRYDDKAVRAALKTAGVRTDVRAEALSL
                     ENMAALFHALQSA"
     gene            6262..7722
                     /gene="glgA"
                     /locus_tag="VFC15_01430"
     CDS             6262..7722
                     /gene="glgA"
                     /locus_tag="VFC15_01430"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001903.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZM08851.1"
                     /translation="MHIAFAASEGVPYSKTGGLADVVGSLPQELAKLGHKVTVYLPRY
                     RLTKLDNPRQVIRSITVPFDDRYRFCSVLDGGVRNGVQFYFIDYPPFFDRDALYGTPL
                     GDYHDNAERFALFCRAVLEATKVLGKPDVFHCHDWQTALIPILLKTLYREDPAFAQTP
                     CVFTIHNLGYQGLFPPEVLPLLMLPWDLFTVAKLEFYGKANFLKGAIAEADYITTVSR
                     KYAQEIQTAEYGFGLEGVLRARASTLTGILNGVDYSEWNPAADPIIAANYSADDLSGK
                     AKCKQDLLKEFGLAADTLLPVVGIVSRFAGQKGFDLIQHVADRMAREEMIMIALGTGD
                     REYEDMFRRLHKQYPQKFAVKIEFNNRLAHKIEAGADMFLMPSRYEPAGLNQLYSLKY
                     GTVPIVRATGGLDDTIEQFDPATGKGTGFKFKEYSGESLLDTVKVAITAFRDTPKWQS
                     LMRNGMAQDYSWAASARDYVKVYERVKQGRPMPIAP"
     gene            7815..8450
                     /locus_tag="VFC15_01435"
     CDS             7815..8450
                     /locus_tag="VFC15_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08852.1"
                     /translation="MFKAALLCLSLSLLVSCSSEKKVATDTASQPAPKPVVKAAQYDT
                     GRTAFQRVYLSARLWAADAKPFRLQSQFTPDAPTAEGKSGMWRASFASPSKRMMKLFV
                     WSGLVGPDAPEQGISFSAEDSWSASNSSTQPFDMGFLKVDSDKAYEVAQKNGGDKLTK
                     KDPKQPVIFVLSWDATKNQLIWHVLYGDNPTEAKLRLEVDATTGEFLRVEK"
     gene            complement(8497..10287)
                     /locus_tag="VFC15_01440"
     CDS             complement(8497..10287)
                     /locus_tag="VFC15_01440"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HZM08853.1"
                     /translation="MILPRIVLAIITTFGVAAVSLSALETPEQEPSKSSGPSAATVNG
                     YQPKWWKEAIVYQVYPRSFKDSNGDGIGDLKGITSELDYIQGLGVNVVWLSPHYDSPN
                     ADNGYDIRDYRKVMTEFGTMSDFDELLRGIKQRNMKLIVDLVVNHSSDEHQWFVESRK
                     SKDNAYRDYYIWRPAKDGKEPNNWTSFFSGSAWKQDPATGEYYLHLFAEKQPDLNWDN
                     PKVRNEVYDLMKFWLDKGVDGFRMDVIPFISKREGLPALPADYIARPQYFYAMGPHLH
                     EYLQEMNSQVLSKYDVMTVGEAFGVTVGQTPSLVDERGNELNMIFNFDAVRLNRNGDA
                     WRDWTLPELKAIYTNLDQTTDVHMWTTVFLSNHDNPRVVSAFGDDSPAHRVASAKVLA
                     TMLLTLKGTPFIYQGDELGMTNYPFTNIDQCDDIAVKNAWKALVLTNQVPPDIFMANV
                     RKVSRDNARTPMQWDSSPNGGFTTAPKAWLAVNPNYKEINAKQELADPNSIYHYFRQM
                     ADLRRKTPALVYGDYQDVDPKNPKIFAYTRTLGKEKYLVVLNFSEDAVSYGLPAGLKA
                     NRLVMSNLATPEDHGETLHLRAWEARVYRL"
     gene            10603..12375
                     /locus_tag="VFC15_01445"
     CDS             10603..12375
                     /locus_tag="VFC15_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZM08854.1"
                     /translation="MRPSSFAPRFLTRLRATGLQYENPRFERVLGVSRVFLGGTALAV
                     DLTHPVAPEPYNQLLIFLLLVYCIQSLGFLIWLMMNPDPGTSFVKIMQLSDVFWPIAF
                     CLFADPPNSLVFVFFLFALMTTAFRFGLYETVLTSVVSALLLVFQEIAVAFGPTELRH
                     LLYTPVEWSRLLLRGGFLLMSGFLLGYLAEREKELRAEIALTNHLLSQARVGNRLSEA
                     LVTISTDLARVFGGERVYEVVSQAGSGRFYRWDVKQVSQPVHEIRELHSTSDGSPLMP
                     EYPHAFYMKRGKDGGVTIDAIDDEGRRLNPSEFKDLPMPVEGAESVLAVKLEVGREWS
                     GRFVLVNGRLGWNREREMRFAQNIVAQVAPALYSIYLFRRLRARAGAMERARVARELH
                     DTAIQSLISIEMQVDVLRRRSNGQPQSAELEHIQKLLREEVLNMRELMQSMRPVEIGP
                     HQFLDFLAQLVERFRSDSGVDARFVSELEEVTLPSSACRELARVVQEGLVNIRKHSGA
                     RSVYIRFGAQNGLWKLVIDDDGCGFPFTGRLTLSELDGLHRGPTVIKERVRAIGGDLL
                     IESSPGHGTRLEITVPQKGYEFYG"
     gene            12368..13042
                     /locus_tag="VFC15_01450"
     CDS             12368..13042
                     /locus_tag="VFC15_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM08855.1"
                     /translation="MDSKVATVRIVIADDHTIFRDGLRRLLEAEPELEVAGEAADGNE
                     AVAQIRQLEPDVLLLDLNMPRMPGMEAMRELSTAGDGKNTKIIVLTAAIERIQIVQAL
                     QLGARGVVMKEAATQLLMKAIRTVMAGEYWIGREAVGDIVEFMRLNASGERPQKNFGL
                     TKREMDILTTIVAGLSNKEIARKFSLSEDTVKHHLTNIFDKVGVGSRLELALFAINNR
                     LTDGQN"
     gene            13267..14160
                     /locus_tag="VFC15_01455"
     CDS             13267..14160
                     /locus_tag="VFC15_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZM08856.1"
                     /translation="MDLSIECALFIVGLAFGSFLNVCISRIPHDLSVVSPPSFCPQCQ
                     ALIRWRDNLPVLSWIILRGRCRDCGLAISIRYPALELLTAFLFVACYASFGFTWLTLK
                     SCVFCFLIVGLIFMDAETGLLPHEFTYSGLGIGLAMGWLAPIDSSGTTFLLSIFGPRG
                     MPSGAELSAIDAAIGAIFGAGFFYLAWALYYLVRRRSGMGFGDIALMGMVGAFLGLRL
                     TTLVTFFAPIMATLFALVILPLAPNAASESSSDDRNSNNSAARSFLFREVPFGVFIGT
                     SSLMALFFGKQIWGWYFGLFR"
     gene            complement(14175..14669)
                     /locus_tag="VFC15_01460"
     CDS             complement(14175..14669)
                     /locus_tag="VFC15_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HZM08857.1"
                     /translation="MELIRRETEIATRGMTPDAWLRAPKGKWNSLQILEHLVLTYTAT
                     TKGLLRTMEAGEPQRGKPDLRQRMRCLYVLGIGRFPAGIEAPQHTVPLKGLGDDPLRL
                     FNNALVAMDATLSDAEKRFGGRTRLLNHPVLGPLTAQAWRRFHLVHGRHHLKQLVARV
                     HGRD"
     gene            14908..16287
                     /locus_tag="VFC15_01465"
     CDS             14908..16287
                     /locus_tag="VFC15_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM08858.1"
                     /translation="MTQSDTNSNAPTLLRELGVSQASAIVVGTIIGSGIFLVPAEMMQ
                     AVGSAQLVYLAWIVGGVLSMAGAITYAELGAMKPQAGGEYVYIRDAYGELPAFLYGWT
                     WFIVAKPGSIATIATGIVRILGTFPALAFFNNAAVWAPFVISYGQLVAIAAAALITWL
                     NYVGVKRAGEFQLVFTILKVVMIVCVGVIGFTAASGTWRNFGTHFTGATGGVAGFMAA
                     LVAALWAYDGWNDLNMVSGEIRRPERTIPIALIAGVGIVAVLYMLTNAAVQYVLPAAS
                     IAASPRPASEATAIAVGLWGAAIVSAGMALSMLVTLNGTIMSGARIPYAMARDGYFFK
                     AMADVHPRFHTPSVALIVQLVLSIVLLLFGGNFRQLFSLTIFAEWLFYMIAASTVFVL
                     RRKEPNTPRPYRTWGYPVVPAVFVLVAAMLLYYTFQENLRNSVWGCGLILSGIPVFLW
                     FARKRVQAE"
     gene            16405..16779
                     /locus_tag="VFC15_01470"
     CDS             16405..16779
                     /locus_tag="VFC15_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08859.1"
                     /translation="MVIDSALTGGWLGGEGGGGEIHGPEQHSGGGEVDAAGVHDAEDF
                     RTAQGEVAAGRGYAKPREGGEAASAGDVVEASAGVEMMAAADTSSDGGALAVAAVGKS
                     VAAKTDDQVRVHKDLRRAIWSG"
     gene            17795..18730
                     /locus_tag="VFC15_01475"
     CDS             17795..18730
                     /locus_tag="VFC15_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08860.1"
                     /translation="MRLRLRKLTLATLVVFTIFVAVQVSGADMKGQVMGGGAPVAQST
                     VTLWAASAGAPKQLAQTKTDNEGRFEVRSAGAPADSSLYLVDTGGEPKARGGGNNSAI
                     ALLAVVGSKPPARVVINEFTTIASVWTHAQFIDGMTIKGAPLQLRIAAGNVPNFVDLE
                     TGGYGATIADALNSTQSPTLANFGTLADVIAGCVTRVQADACNSLFEAARPPSGGLPT
                     DTLNTAESIALYPWHQPDKVFALLAKLYPIPQGKRELRPAPFMPYLTFAPSAWVLPLR
                     FAGGGLSAGGKLMIDSEGNIWTADNFQVGAQAQDT"
     gene            18830..>19319
                     /locus_tag="VFC15_01480"
     CDS             18830..>19319
                     /locus_tag="VFC15_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08861.1"
                     /translation="MAIDPSGKVWFSGFGGSPAISVFDNDGKPLSPREGFTFGGELGQ
                     LQGTIATPSGDIWAADSVKSQLVYLPKGDASKGKIFCKNPSSDPLKNPCQLLIPFGLA
                     IDQKDNIWVTNILGDHVTRFPASDPTKAVTFKTGFSGSGLAVDSLGNVWITNKLGNSE
                     RGR"
BASE COUNT         3897 a         5308 c         5869 g         4245 t
ORIGIN      
        1 aaacagcaca gtgatccgcg taaccggatt ctgcatgcca tcggcacgac gctgggcttg
       61 acggtcgtgt cgggcgctct gattcttggt cacccgtggt acgccttgct gttctttccc
      121 attggatacg ggtttgcctg gaccggacac tttgttctgg agaagaacaa gccggcctcg
      181 ttcggccacc cgttctggtc gttcatcagt gatttccgca tgctgggact gatgtttacc
      241 ggacggctga aggccaggat gtagctcaac gtccatagtt gtacaacctc acttctgggc
      301 cgctcctgct acactcggcg caagatcatg agagtcctcg gcatagattg cggcggcgaa
      361 tacaccggct acggtgtggt cgagcaggac gatcaaggcg tgctgctgca cgtatgcagc
      421 ggagcgatcc ggttgctacc gcgcgaggca ctggaaattc ggctgatgaa aatctgccgg
      481 ggcttgaccg aggtgatcgg ttcgttctcc ccggaagtcg tcaccatcga agatgtgttc
      541 tacgccgtga atgcaaagtc ggcgttaaag ctgggacatg tgcggggtgt agcgatgttg
      601 accgcggccc aagcgggact gtccgtgatt gcgtactcgc cgctgtccat caaatcagcg
      661 gtggtgggtt acggccgcgc ggaaaagtcg caagtacagt tgatggtcgc gcgcctgctg
      721 aatctccccg agcctccgga acccccagac gttgctgacg cgctggccat cgcaatctgt
      781 catctgcata ccgcggcaac gctgcggcga caacacgcga caaccaagtg agtttggcaa
      841 tgcgctttcc gcaaaccacc agcagactta ggttaattcg actgggactt gattccagac
      901 atgaggtatc ccaatgccgg tgcgtatgac tttttcccgg ccagtaactg cgggagtccg
      961 gcgcaagaat ctagatgggt gctatgtcat cggagaatgg atggtagtac ccgaagagcg
     1021 gcgcatcgag aaagacggcc gcgtaatcgg aatgagccga aatcttatgg gagggctgct
     1081 gtgcctcatc gatgcgcagg gtgcgtctgt ttccctggag cgtctcaagg gtgcaatgtg
     1141 gccttcgagt atcgtcagcg acgaaacggc tagatcttac atggatactc tgcagagggc
     1201 actggaaacc gactccgcac ctggatccgt cgtagaaagt aatcccgaat gtggataccg
     1261 gcttctggtg aatgctcgtc ctgctggtcc cgacgatgct ccagagtccg agctgacaag
     1321 acagcaggtg cctgccgatc cgtttgaggt caatgccgat gtagcggggt acatcttgag
     1381 ttaccattcc aatctcatga ccgagttgga acatcgggca caggggcact ttcacgccaa
     1441 aatgaagatg accggatggg gcgacaggtc gccacagttg caggcgtcgg tgtcgcagga
     1501 atactcccgc tatctagcga ccgatcctgc cgtactggcg ctggtgaacg acggctatga
     1561 agcctttgcg gaaagggcag cgaaccgcat tctgaaggaa tacggcgacc gcttgaatct
     1621 caaccgttgc ccaagctgcg cttgcatagc gcggacaccg aaagcgcgcc aatgccgttt
     1681 ttgtggccgc gattggcacg acggataaaa gagactttca tcgcaggcga gcttacttgc
     1741 agtatcccca cgtcaccctt ccttagattt ggttcaggaa gaatcccctc gttgttagga
     1801 agcgccgcgc gcgttcggtt agaatcattc gcgagaatga gattgttgcg tctgaagtat
     1861 gtcctaacgt tctggctggt atgtgcgctt agtgcaaccg cccaggtggt tcatcaagtc
     1921 gaccctgcgg agacgcctac cggctcctat gtcacgttca agttcgactg ggaccagggg
     1981 cgtccctggc tgcgatattc cattgcggtt gacgatgccg gcaatgccca cttcgaaggc
     2041 gtgggaaacc cgattgagag cggtgacagc gatgcgttca cggaagactt caccatgacc
     2101 gacatcaacc gtcggaaaat cttcgacctg gccaagaagc tggattattt tcaaggaaac
     2161 ttcgaggcga aacagaagaa catcgccaag acgggccaga agacgctggg ataccacggg
     2221 catccggcgg gtggcgggca aacaatagag cactcctcga cctacaacta ctcgccgaat
     2281 tccgacgtgg aagaactgac tcgggtcttt caggcgattg ccacgacgct cgactatggg
     2341 cgcaagctgg cgtttaagtt ccgctacgac aagctgggct tggacgcaga gttgaaagat
     2401 ttacaagata tgcaggcgag tcactacatt gaagaactgc aggcgattga gccaatttta
     2461 cagaaaatcg ccgatgaccc gaacatgatg catatcaacc gtgtgaccgc cagacagatg
     2521 ctgaaagcga tggggaccac cggggtgccc agtcagacga cgagtgagcc gtaaatctaa
     2581 cgggtgaggt tgctgttgct ggatggcgcc gctggttcag agccgggaac ggctcttcgc
     2641 tttggcatcg atttccggca acttcgcatc aaagcggtgt ttaataggtt aggtatggaa
     2701 cggacaggga tgggtgtccc cgtcgcaatg aacagtagtc ttccaacaag tttctggatc
     2761 gggtgaatgc ggtcgggggg ggtagcggta tggcaaagcg acaaaaatgt ggattggccg
     2821 tgatggcggc gatgctgtta gcagttgtgt ctgcccaggc gacatcgcac gtgaggatcg
     2881 tccggttgag ctacgaagac ggcaacgtgc agatgagtcg aggcacgggc cagggtctgg
     2941 agcgcgccat cctgaactcg ccaattgtcg agggttcaag cgtggtgacg ggcagggacg
     3001 ggctggcgga agtcgaattc gagaacaaca gcacggttcg gttgggcgaa gccacggaca
     3061 tgagtttccg gcagttgctg ataaatgacg ctggcgaaaa ggtcaacgaa gtcgaactgg
     3121 tgcgtggcac catgtacttc gatacccgct ccggcaagga caacgtctat cgcgtcgtcg
     3181 ctaccgaccg cacctttgtc gtgcagcgca attcgctggt gcgattcatg atgagcggag
     3241 accaggttga ggttgccgtc atgaatggaa ccgcgcagct ggcaaacagc gccgagccgg
     3301 tcaagcttaa gaagaacgaa acccttactt cggactccac gaatccccag ggcttcacca
     3361 tggccaaggg tgtagacagc acgccggttg accgctggaa cagtgagcgt gccgcctacc
     3421 agaccgtcta tgcgtataac aacacgggat acgggtcgta cggctcgccc ggactgggaa
     3481 gctacggttt ccaggatctg gcgtattatg gcggattctt gaaccttccg ggatacggca
     3541 tggtatggca accgtacggc gcctcgaatt ggttgggatg gaacccgtac ttgtgcggcg
     3601 catgggcgtt caccccggga tttggctatg catgggcttc ggcctaccct tggggctggt
     3661 tgccctatca ctatggcgcg tggggatatt cgtcggggat agggtggttc tgggcaccgg
     3721 gcaatacaat gggacacggc ggaatcgcga ccaattggca gccgacagct ccggtgatcc
     3781 atggaccgcc cgggtacact gctccgctgc ctcctaccac caccagcgtc ggttctcaat
     3841 ctacggtttt ggtaggacgg attgcacgca cgccagccta tatcccaggc ggcccagtgc
     3901 ccccggattt tcgcagtgtc atcggtcgtt cgagcatgat tggaacgacc ggtacggcga
     3961 ctggcgcgac aggggcggca ggttcggtcg cgcgaaactc caaggcggct agcttgatcg
     4021 gctccactac caccgcacca gcgggcaata cgttcgcaac tccgggagcg aactcatcaa
     4081 ccgctggttc gagccactct acctctggga gttcctacaa tcgctctggc catgtgtttg
     4141 tggtacctac cgcaccggtc tggacgtcgc agggctactg gacgccgggc ggaggcagcg
     4201 caacttcagg acaagcatct tgggctcccg cacacagttc gggcggcacg agtcgtgcga
     4261 ccggcaccgg tggagcagcc cacagcaccc ccaaatagtc cctttgataa aacgaaatgg
     4321 ggcggctttg cagccgccct ttgttttgca tcgagggtga tggaatcaat acactgcaat
     4381 cccggcatag cccaccacca tgtcgagatc ttctgacaca tctccagacg tcgcatagcg
     4441 tatgagttct gcctgggtgg ctcccatctt gcgaacggca gtgagcatga tggtggcggg
     4501 accatagccg cacatgctga tgttgccctg gcgcaccgtg tcgtacaggc cccgcggatc
     4561 gagcgcgagg acctggtcta tggcgcgacg gtctttcacc cgcgtaacgc tgtcgctctc
     4621 gtaatggttc atgtcgctgg aagcgatcac cagggcaggt tcggacagac cagacaacag
     4681 gctcccaatc gcgtcgccga gctcgctcaa gtcttcgaag ttgctggtgc cgaccgtgat
     4741 gggtacgaac tgaaagccgg gccgcaggac ctgcaggaac gggagctgca cctccagggc
     4801 atgttcgtag cgatgcgctg ccgggtcttc ggtgagcagg ggcacgacgg atttcagtgc
     4861 atcagcgagc tcggcatcga cgggtacgtc tcccagtgga gtgtgccatg cgccctggct
     4921 catgatggcc agaggctctc ccatcccggt gtggttagga cagagaatga cgcagcggcg
     4981 aggcaatata agtctgcggt aaacggctcc cgcaaccgcg cctgaataga tgtagccggc
     5041 atggggaact acgcacccta ccgcgtgtaa tttgatttcg attgccactt gtcctgcaac
     5101 ggcgcgtgga gtggtgaagg tctccacttc ggtgcgcaag tgctgcgcgt ttcccggata
     5161 gaagcgtccc gcaacggctg gctggcgaat agcggtgatc atggcgatcg ctccttcgca
     5221 tgccaaagtt ccatgttctg ggactaaggc tttcaaattg aattttacgc cgactgcaag
     5281 gcgtggaata acgcggccat gttttcgaga gagagagctt cggcgcggac atcggtgcgc
     5341 actcctgcgg ttttcaaggc tgcgcgcacc gccttgtcgt cgtagcgccc cttcaagttg
     5401 ttgaccagtg ttttacgctt ctgcgcaaaa gccaatttca gcatgtccag aaagggcccg
     5461 gcttcgatct gaagttcagc aaggcgcggt gccatggtca gcctcagcac gctggaatgc
     5521 actttgggcg gaggatcgaa ggcgccggga ggaagagtaa ataggttgtc gacgcgcgca
     5581 aatagctgcg cggtcgccga cagcaatccg taatcgcggc tacccggctc ggcggagatg
     5641 cggtcggcaa cctcgcgctg caccatgatg acggcgcgcc cgatgttccg gtgctcctcg
     5701 agaatgcgca acacgatgtc ggaggtcacg tagtacggca ggttgccgat gatgtcgact
     5761 gtttcgggct gcgtggggcg aaggtcgtgt aaagggccgg ggcggcgacc gaccatggaa
     5821 atccattcca cggtgacgaa gtcagactcg agaatctcga cattctgcaa agtcgcgtag
     5881 cgcatgcgca tttgagccgc cagcacgtgg tcgagttcga tgccgatgac gcggcgggcg
     5941 cgcttgacca actggtcggt gagcatgcct cgacccggtc cgatctcgac cacggtgcgg
     6001 ctcgacacgt caccgagtgc gtcgacgatc cgccgggcgg ccgattggtc gatgagaaaa
     6061 ttctgcccca gcttggactt gcgctttccg ccagtggtat ttacctcttt ttcatgggac
     6121 gcggacttga ccacgttgac cctctttcga ccccgcgatt agacacaact gtttcgctcg
     6181 ttgcaatatc ataaacgggc agcctgcatt gcgctgttcg gcatgttgcc cgagggaagc
     6241 tcgaccgagc ttggaggttc catgcacatc gcctttgcgg catcggaagg tgttccgtat
     6301 tcgaagaccg gtgggctcgc agacgtggtg ggatcgctac cacaggagtt agcgaaactc
     6361 ggacacaaag tgacggtcta ccttccccgt taccgtttaa ccaaactgga caacccgcgc
     6421 caggtgattc gcagcatcac ggtgccattc gacgatcgct atcgcttctg ctcggtgctc
     6481 gacggcggcg tgcgcaacgg agttcagttt tatttcatcg actacccgcc attcttcgat
     6541 cgtgacgcgc tctacggaac tccgctgggc gactatcacg acaacgccga gcggttcgcc
     6601 ctgttctgcc gggcggtgct cgaggcgacg aaagtactgg gtaagccgga cgtgttccat
     6661 tgccatgact ggcagaccgc gctgatcccg attttgctga agacgctgta ccgcgaagac
     6721 cctgcctttg cgcaaactcc atgcgtgttt accatccaca acctgggata ccaaggcttg
     6781 tttccgccgg aagttctgcc gctgctgatg ctgccgtggg acttgttcac cgtggcgaag
     6841 ttggagtttt acggcaaggc gaacttcttg aagggcgcca ttgccgaagc tgactacata
     6901 accacggtga gcaggaagta cgcacaggag attcagactg ccgaatacgg ttttggcctg
     6961 gagggggtgc tcagggcacg agcgagcacg cttaccggca ttctcaacgg agtcgattat
     7021 tctgagtgga accctgcggc cgatccgata atcgcagcca actattcggc ggacgatctt
     7081 tccggcaagg cgaagtgcaa gcaagacctg ttgaaagagt tcggactggc cgccgacacc
     7141 ctgttgccgg tggtgggaat cgtctcgcga ttcgctgggc agaagggttt tgacctcatc
     7201 cagcacgtgg cagaccggat ggcgcgcgaa gagatgatta tgattgcgct cggtaccggc
     7261 gatcgcgagt acgaggacat gttccggcgc ctccataagc agtatcctca aaagtttgcc
     7321 gtaaaaatcg agttcaacaa ccgtctggca cacaagatcg aagccggcgc tgacatgttc
     7381 ctgatgccgt caagatacga gcctgcggga ctgaaccagc tttacagcct gaaatacggt
     7441 acggtgccca tcgtgcgtgc gaccggggga ctcgacgaca caatcgagca gttcgatccg
     7501 gctactggaa agggcactgg attcaagttc aaggagtaca gtggagagtc gctactcgat
     7561 accgtgaagg tggcgattac tgctttccgc gacacgccaa aatggcagag cctgatgcgc
     7621 aacggcatgg ctcaggacta ctcgtgggcc gcatccgcac gcgactacgt gaaggtctac
     7681 gagcgggtca agcagggaag accaatgccg atcgcacctt aggaattcca tagcaatgcc
     7741 gcgccgacag cttcgtcaga tgccgggatg gttgtagcta attcaaatga gagagtcgtc
     7801 tggtgcctaa agaaatgttc aaagctgccc tgctctgcct atcgttgtcc ctgctggttt
     7861 cttgttcttc ggaaaagaaa gtcgcgacgg acacggcttc ccaaccggcc cccaagcctg
     7921 tcgtgaaggc agcgcagtac gacactggac gcaccgcgtt ccagcgggtg tatctgtcgg
     7981 cgcgcctgtg ggcggcggac gcgaaaccgt tccgcctgca atcgcagttt acgcctgacg
     8041 cgccgaccgc tgagggcaag tccgggatgt ggcgcgcgtc gtttgcctcg ccgtcgaagc
     8101 ggatgatgaa actgttcgtg tggtcgggac tggtcggtcc cgatgcgcct gagcagggga
     8161 ttagtttttc cgcggaagac agttggagcg cgagcaatag ctcgacccag cctttcgaca
     8221 tgggattcct taaggtggat agcgacaagg cctacgaagt cgcgcagaaa aatggcggcg
     8281 acaagctgac caaaaaggac cccaaacaac cggtgatctt tgtactctcc tgggacgcaa
     8341 ccaagaacca actgatttgg cacgtcctgt acggagataa tccgaccgag gcgaagctcc
     8401 gcctcgaggt ggacgcgacc accggggaat ttctccgtgt cgaaaaatag gcgcccggct
     8461 tacgcaagac ctgtaaagcg tcgaacatcg caagacctac agcctgtaaa ctctggcttc
     8521 ccaagctctc aggtgaagtg tctctccgtg atcctctggc gtggccagat tcgacattac
     8581 caaccggttc gctttcaggc ccgcgggaag gccgtaggac accgcgtcct cagagaagtt
     8641 cagcacgacc aggtattttt ctttccccaa ggtgcgagtg taagcgaata tcttcgggtt
     8701 cttcgggtcc acatcttggt agtcgccata gacgagggcc ggagtctttc gccgcaggtc
     8761 cgccatctgc cggaagtagt ggtaaatcga attcgggtcg gctagctcct gcttggcgtt
     8821 aatctccttg taattcgggt tcaccgctag ccacgccttg ggagcggtgg tgaagcctcc
     8881 gttcggcgaa ctgtcccatt gcatcggggt gcgggcgttg tcgcggctga ccttgcgcac
     8941 gttggccatg aagatgtccg gcggaacctg gttggtcagc accaatgcct tccaggcgtt
     9001 cttaaccgcg atgtcgtcgc actggtcgat gttggtaaac ggatagtttg tcatccccaa
     9061 ctcgtcgccc tggtaaatga atggcgtgcc cttcagcgtc agcagcatcg tggccagtac
     9121 cttggccgac gccacgcgat gcgcgggtga gtcgtcgcca aaagcagata caacacgcgg
     9181 gttatcgtga ttggacagaa aaactgtggt ccacatgtga acgtccgtag tctgatccag
     9241 attggtgtaa atcgctttga gctccgggag cgtccaatcg cgccacgcat cgccattgcg
     9301 gttcaaccgc accgcatcga aattgaagat catgttcagt tcattgccgc gctcatcgac
     9361 gagtgaggga gtctgcccca cggtcactcc gaatgcctca ccaaccgtca tcacgtcgta
     9421 cttggatagc acttggctgt tcatctcctg caaatattcg tggagatgtg gtcccatggc
     9481 atagaagtac tgtggccgtg cgatgtaatc cgcgggcagg gcggggagtc cctcgcgctt
     9541 ggagataaac ggaatcacgt ccatgcggaa gccgtcgacg cctttgtcga gccagaactt
     9601 catcaagtcg tagacttcgt tgcggacttt gggattatcc cagttgaggt cgggctgctt
     9661 ctcggcaaac aggtgcaggt agtattcgcc cgtcgcggga tcctgcttcc acgctgatcc
     9721 cgagaaaaac gacgtccagt tattcggttc cttgccgtct ttcgccggac gccaaatgta
     9781 gtagtcgcga tacgcgttgt ccttggactt cctgctctct acgaaccact ggtgctcatc
     9841 gctggagtgg ttcaccacca ggtccacgat cagtttcatg ttccgttgct tgatgcccct
     9901 caggagttca tcaaagtcgg acatggtgcc gaattcggtc atcacttttc ggtaatcgcg
     9961 aatgtcataa ccgttgtcgg cgttcgggga atcgtaatgt ggactcagcc agacgacgtt
    10021 gacgccgaga ccctggatgt aatccaactc cgaggtgatg cctttcaaat cgccaatccc
    10081 atcgccattc gagtctttaa acgaccgggg atagacctgg taaacgatgg cctctttcca
    10141 ccatttgggc tgatacccgt tgacggttgc ggcagatggc ccgcttgatt tggatggttc
    10201 ctgttctggc gtttcgagcg ctgacaggga aaccgcggcc accccaaagg tggtgataat
    10261 agccagtacg attcttggaa ggatcacgag aagtcctcgg atattttgga tgtcacttaa
    10321 gtatcaccgc tatgctacat ccaagtttga ggacgtgtgg gcatccgttt gtaactgtca
    10381 ggttgctttg gaaagcggtt gacgccgaac aactagagag ggaactactt tccctgcacg
    10441 tcgaagtgaa tttcgtcgga acgaagcgcc gtctctaact cgttttttaa caattagatg
    10501 catacggtac agacgccgtc tcacaccaac ggtgtgttgc agtttggctc tttagtgtgc
    10561 ataatcttgc agcgtgagct tttcgccgat acaaggacgt tcatgcgccc ttcatcattt
    10621 gccccgcggt tcctgacccg gctgcgggct accggtctgc agtacgaaaa tccccgtttt
    10681 gagcgggtgt tgggggtttc gcgcgtattt ctgggcggaa cggcgctggc tgtcgacctc
    10741 acccaccctg tcgctcccga gccttacaac cagcttttga tcttcctgct actggtctac
    10801 tgcatacaaa gcttgggatt tttgatctgg ctgatgatga atccggaccc cgggacgtct
    10861 tttgtgaaga tcatgcaact gagcgacgta ttctggccca tcgcgttttg cctgtttgcg
    10921 gaccccccca acagcttggt ttttgtattt tttcttttcg cgctcatgac gactgccttt
    10981 cgttttggac tctatgaaac agtattaacg tcggtagtta gcgcactgct gctggtattc
    11041 caggaaatcg cggttgcttt tgggccgacg gaactgcgcc acctcttgta cacgccggtc
    11101 gaatggtcac gactcctcct acgtggcgga tttttgctca tgagtggttt tctgttgggc
    11161 tatttagctg agcgcgagaa agaactgcgg gccgaaatcg cgctcaccaa ccatctactc
    11221 tcgcaagccc gcgtcggcaa tcggctaagc gaagcactcg tcacgatttc gactgacttg
    11281 gcgagggtgt ttggaggaga acgagtgtat gaagtggtgt cccaagctgg ttccgggcgc
    11341 ttctaccgtt gggatgtcaa acaggtttcg cagccggtcc acgagattcg ggaacttcat
    11401 tccactagtg acggttcgcc gctgatgcca gagtatcccc atgctttcta catgaagcgg
    11461 ggaaaggacg gtggagtaac gattgatgcc atcgatgacg aggggcggcg gctaaatccg
    11521 tcggagttca aagacttgcc gatgccggtg gagggagcgg agtcagtact ggcagtcaag
    11581 ttggaggtag gccgggaatg gagtgggcga tttgttctgg tgaacgggag actggggtgg
    11641 aaccgggagc gcgagatgcg ctttgctcaa aacatcgtcg cccaggtcgc tcctgctcta
    11701 tactccatct accttttccg gcggttacgc gcgcgcgcag gggcgatgga gcgggcccgg
    11761 gtagcccgcg aactgcacga tacggccatt caatcgctga tcagcataga aatgcaggtg
    11821 gatgttctgc ggcgacggtc caatggtcaa cctcaatccg cggaactgga gcacattcaa
    11881 aagttgttgc gggaagaagt gctgaacatg cgggaactga tgcagagtat gcggccggtg
    11941 gaaattggac cacatcaatt tcttgacttt cttgcccaac tcgtagaacg ttttcgcagt
    12001 gattcgggcg tcgatgcacg gtttgtttcc gagctggaag aagttacact tccttcctct
    12061 gcctgccggg agttggcccg ggtggtgcag gaaggtttgg tgaatatcag gaagcacagc
    12121 ggggcgagat cggtgtacat tcgtttcggt gcgcagaatg ggctgtggaa gctggtaatc
    12181 gatgatgacg gatgcggatt tccgttcacc ggcaggctga cactttcgga gttggacggt
    12241 cttcaccggg gcccaactgt gatcaaggag cgggttcgag ccatcggtgg cgatctgctg
    12301 atcgagtcaa gtcccgggca cggtacacga ttggaaatta cggttcctca gaaggggtac
    12361 gaattctatg gatagcaaag tcgctacagt gcgaatcgtc atagcggacg atcatacaat
    12421 ttttcgcgac gggctccgcc ggttactgga ggccgaaccc gaacttgagg tagcagggga
    12481 agccgccgac ggcaacgaag ccgtggcgca aatccgacag ctggaacccg atgttctgct
    12541 gttggacctc aatatgccca gaatgccggg catggaagcg atgcgcgaac tctctaccgc
    12601 cggcgacggc aagaacacca agatcatcgt ccttacggcg gcgattgaac gcattcaaat
    12661 cgttcaggcg ctgcaactcg gagcgcgagg cgtggtgatg aaagaggccg ccacccagtt
    12721 gctgatgaaa gccattcgga cggttatggc gggcgaatac tggattggcc gggaggcggt
    12781 gggcgacatc gtcgagttca tgcgcctgaa tgcgtcaggg gaaagaccgc agaagaattt
    12841 cggcctgacc aagagggaaa tggacatctt gacgactatc gtcgcaggtt taagcaacaa
    12901 ggaaatcgcc cgcaaattct cgctaagcga agatacggta aagcaccact tgacgaatat
    12961 cttcgacaag gtcggagtcg gcagccggtt ggaattggcg ctgtttgcca tcaacaaccg
    13021 cctgacagac ggacagaact gaccagcctc atcgtcttcc cgcgtcgaag ggccggtttg
    13081 tctctccggt cctttgcagt ggggagatgc atcagcgggt gccgcaatca agcctacggg
    13141 ccgccgcagc tatggagaac ttgaggctgg gagccgctac ggaaattgct gagtgaactt
    13201 ttccggcgct cccggcgttc tgtcgctctt ccgttgcccg cctgcgacga cgtactctag
    13261 taggcattgg acctctcaat cgaatgcgcg ctgttcattg tgggactggc gttcggcagc
    13321 ttcctcaacg tttgcatcag ccggattccg cacgatttgt cggttgtctc cccaccgtca
    13381 ttctgcccgc aatgccaggc attgattcgc tggcgcgaca atctccccgt gctcagctgg
    13441 atcatccttc gcggccgatg ccgcgattgt ggactggcca tttcgattcg ttatcctgcc
    13501 ctggagctac tgaccgcatt cctgtttgtg gcgtgctatg catcgttcgg attcacgtgg
    13561 ctgacgctga agtcttgcgt gttctgtttc ctgattgttg gactgatctt tatggacgcc
    13621 gagaccggcc tgcttccgca cgaattcact tactcgggat taggcatcgg actggcgatg
    13681 ggatggctgg ctcctatcga ctcgtctggc acgacttttc tactgagcat atttggcccg
    13741 cgcggcatgc catcaggtgc agaactgtcg gcgattgatg cggcaatcgg cgcgatattc
    13801 ggagcagggt ttttctattt agcctgggca ttgtattacc tggtgcgccg gcgcagtggg
    13861 atgggatttg gtgacatcgc cctgatggga atggtgggag cgtttctcgg gctaaggttg
    13921 acgacgctgg tgaccttctt cgctccgatt atggctacgc tgttcgcttt ggtgatactt
    13981 ccattggctc caaatgcggc ttctgaatct tccagtgacg atcgcaattc gaacaactcg
    14041 gccgccaggt catttctgtt tcgcgaagtg cctttcggtg ttttcatagg cacaagctca
    14101 ctcatggcat tgttctttgg aaagcagatt tggggttggt atttcgggct ctttcgctga
    14161 acgtttctgc agacttagtc ccgtccgtgc actcgggcga ccagctgctt gagatggtga
    14221 cgtccgtgga ccaggtgaaa gcgccgccac gcttgcgcgg tcagagggcc gagcacggga
    14281 tggttgagca atcgggtgcg gcctccgaac cttttttctg catcgctcag cgtggcatcc
    14341 atggcgacca gcgcgttgtt aaagaggcgc agcggatcgt cgcccagccc cttgagcggc
    14401 accgtatgtt gaggggcttc gatccctgca gggaaccggc caattcccag aacgtagagg
    14461 caccgcatgc gctgccggag atccggtttt ccgcgctgag gctcgccagc ctccatggtt
    14521 cgcagcagac ccttggtagt cgcggtgtag gtcaggacga gatgctcgag aatctgcagc
    14581 gaattccatt ttcccttggg cgcgcgcagc cacgcgtcgg gcgtcatccc acgcgtcgcg
    14641 atttcggttt cgcggcggat caattccaat gccggagaca caactcaacc cattatgcgt
    14701 tcgtaacagc caatcctgag taatcacact atatgaagcc gaggtcattt gttcaagcga
    14761 tgtgtcgagt cgatgggatt cgaggtatgg agcgcagata ctgcaagttc cggcaacgcc
    14821 ttcctggttg ctgccgacca gcccgctcgg gaactcagtg atcatttata gttcgagtct
    14881 gctgctctcg taacgcgaca cgaaaaaatg acacagagcg atacaaatag caatgctccc
    14941 accctgcttc gcgaattggg cgtaagccag gcgagcgcaa ttgttgtcgg caccatcatt
    15001 ggcagcggca tcttcctggt tccggccgaa atgatgcagg ccgtgggctc ggcccaattg
    15061 gtttatctgg cctggatcgt cggcggtgtg ctgtcgatgg ctggggcaat cacgtatgcc
    15121 gagctgggcg cgatgaagcc gcaggcgggc ggggaatatg tgtacattcg cgatgcctac
    15181 ggagaactgc cggcgtttct gtacggctgg acgtggttca tcgtcgccaa acccggttcg
    15241 attgcgacga ttgctacagg tatcgtgcgc attctcggaa cgtttcctgc actggccttc
    15301 ttcaacaatg cggcggtgtg ggcgccgttt gtcatcagct atggacaatt ggttgctatc
    15361 gccgcagcag cgctgatcac ctggctgaac tatgtcggcg tgaaacgtgc cggcgaattc
    15421 cagttggtct tcacgattct gaaagtggtc atgatcgtct gcgtcggcgt gatcggattc
    15481 accgcggcga gcggaacatg gcgaaatttc ggcacccatt ttactggagc gacgggcggc
    15541 gttgcgggct tcatggcggc gctggtggcg gcgttgtggg cttacgacgg atggaacgac
    15601 ctgaacatgg tgagcggaga gatccgccgt cccgaacgca cgatcccgat tgcgctgatc
    15661 gccggcgtgg gcatcgtggc cgtgctgtac atgctgacga atgcggctgt gcagtatgtg
    15721 ctgccggccg ccagcattgc ggcctcgccg cgtcccgcgt cggaggcaac tgctattgcg
    15781 gtcgggcttt ggggagcggc gatcgtttct gccggcatgg ctctgtcgat gctggtcacg
    15841 ttgaacggca ccatcatgag cggagctcgc attccttacg ccatggcgcg cgatgggtat
    15901 ttcttcaagg ccatggcaga tgtccatccc cgctttcata ctccgtcggt tgcactgatc
    15961 gtgcagctcg tactgtcgat tgtgttgctg ttgttcggcg gaaacttccg gcaactgttt
    16021 tccctcacga tttttgccga gtggctgttc tacatgatcg ccgccagcac ggtgtttgtg
    16081 ctgcggcgca aggagccaaa tactccgagg ccgtatcgca cctggggata tccggtggtt
    16141 ccagcggtgt tcgtgctggt tgcggccatg ctgctgtact acacgttcca ggagaacctg
    16201 cggaattcgg tgtggggttg cgggctgatt ctgtcgggca tcccggtctt cttgtggttc
    16261 gcccggaagc gagtgcaagc ggagtaggca ttgttaaagg ggtaccccct cccctcgcct
    16321 tcttgtgttt tcaggtactt gtgaggggtt ttcggtgcag ctttgggaaa acaaagggtt
    16381 tagtggtatg taatagaaaa cattgtggtt attgactcgg cgctgacggg tggttggctc
    16441 ggcggggagg gcggcggcgg tgagatccat ggcccagagc aacattcggg cggtggcgaa
    16501 gtcgacgctg ccggtgttca cgacgcggag gatttccgaa cggctcaagg cgaggtcgcg
    16561 gcgggtcgtg ggtacgcgaa gccgagggaa ggaggcgagg cggcgagcgc gggcgatgtt
    16621 gtggaggcga gtgcgggcgt cgagatgatg gcggcagcag acacgtcctc ggacggcggg
    16681 gccctggcag tggcggccgt cggaaaaagt gtggcggcaa agacggatga tcaggtgcga
    16741 gttcataaag accttcgcag ggccatttgg tcaggatagg gggacggtgg ggatggagtg
    16801 aaggggaaag tagaggttga cgttacgaag gtaaccagat tccccgaatt gggaagaatg
    16861 cgggagctcg ggctgaagcc cacgtcggca tctgtcgtcc ccgcagggac tccccatcta
    16921 taaaacgagc tttcccagcg ttgaagagtc tgttacaaaa cgctgccgtg cctacggcac
    16981 tcccatgaat tctcccactc tacccagcac tccgccttcg gcttcgtgct gggctagact
    17041 attccgcgcc tccggcgctc gcattcgtgg cgttcgttcg acgccacaag ccgaagcgcg
    17101 agtttcgtaa cagcctctga aacgctgggc tttcttatgc cgtcccgccg ggcgggactg
    17161 gagtgtcgcg ggtaccgggg gcaaattggt gtcccgggtc tgcatcgccc accccttcga
    17221 cttcgctcag ggcaggctgt cgctgcgtga caagggtggg gcaccggcgt ttttgagccc
    17281 gtgctaccat gccaccgtgt tcaattttgg caaaccgaaa acagttggcg actggatcgt
    17341 tcatgtcgcg ggtggcgtgg tcgcattact cctcgtatgg tggatgctac ggagctatgt
    17401 cctgtaatcc ccgctggctt gaataacgaa ataacgattt cgtcctttcc ggttgtgttg
    17461 cccttttgcc actgttacca gtgctaaggg ctatagcccg tgacccatag ttggcgataa
    17521 atcgccatgg cataggtatt ccggctgagc gccgccgttc gggattacag acgacatggg
    17581 ctgattgcaa tagctatctg gaacggacca gtgctccgaa gctgcatctt aacgacagta
    17641 ccgaatgcat tggacgcaag agcctgcacc tttctaaact acgctgtaca cattcaacct
    17701 gttagttggc gcggcgatta agcgtcgtcg tgttcccaag atgcacttct gccttagagt
    17761 ttcagccgct taataggggg gattgaaaat gcatgtgcga ttacgacttc ggaagctgac
    17821 gctggcgacg ctggttgttt tcaccatttt cgttgctgtt caggtttctg gtgccgacat
    17881 gaaaggtcag gtgatgggag gcggcgcgcc cgtcgctcag tcaaccgtga ccttgtgggc
    17941 agcgagtgct ggagcaccta agcaactcgc tcaaaccaag accgacaacg aagggcgatt
    18001 tgaggttcgc tccgcaggcg cgcccgccga ctccagcctc tatctcgtgg acacgggtgg
    18061 tgaacccaaa gcccgggggg gcggaaataa ttccgctatc gcattgttgg cggtggtcgg
    18121 aagcaaacct ccggcacgcg tcgtcatcaa cgagttcacc actatcgcgt cggtatggac
    18181 gcacgcgcaa ttcatcgacg gcatgacgat caagggggcg ccgctccaat tgcgcattgc
    18241 agcgggcaac gtgcctaact tcgttgacct cgaaaccggg ggctacggcg ctaccatcgc
    18301 cgacgcgctg aacagcaccc aatcgcccac cctggccaat ttcggcacgc tggctgacgt
    18361 catcgccgga tgcgtcacgc gagtgcaggc ggatgcgtgc aacagtctct tcgaagctgc
    18421 ccggcctccg agcggcggtc ttccgaccga cacgctgaac accgccgagt cgatcgcgct
    18481 ttacccgtgg caccaacctg acaaggtctt cgcactgctg gcgaagctct accccattcc
    18541 gcagggcaag cgcgagctgc gtcccgctcc gttcatgccg tatctgacgt ttgctcccag
    18601 cgcctgggtc ctgccgctgc ggttcgctgg cggcggctta tctgccggcg gcaagttgat
    18661 gatcgacagc gagggaaata tctggaccgc tgacaacttc caggtcggag cgcaggccca
    18721 ggacacctaa tgggcgggcg gcatctccaa gttcgcgccc aacggcactc cgctctcgcc
    18781 gtcgctcacc ggcttcaccg gcggcggcct ggcgggtccc ggattcggct tggcgatcga
    18841 ccccagtggc aaggtttggt tttccggctt cgggggtagc ccagccatct cggtcttcga
    18901 caatgacggg aagccgctgt cgccgcgcga aggcttcacc ttcggaggcg agctcggcca
    18961 gttacagggc accattgcga cgcccagcgg agacatctgg gctgccgact cggtgaagtc
    19021 gcaacttgtc tatctaccta agggcgatgc ctccaagggc aagatcttct gcaagaaccc
    19081 cagcagcgat cccctgaaaa atccctgcca gctcctgata cctttcgggc tggcaatcga
    19141 ccagaaagac aacatctggg tcaccaacat tcttggcgac cacgtcacgc gcttcccggc
    19201 ctccgaccca accaaggccg tgactttcaa gaccggcttc tccggcagcg gcctcgcggt
    19261 cgacagtctc ggcaatgtct ggatcaccaa caagctgggc aattccgagc gcggccggg
//
LOCUS       DASRKK010000071         5504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_785989, whole genome shotgun sequence.
ACCESSION   DASRKK010000071 DASRKK010000000
VERSION     DASRKK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785989"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..2364)
                     /gene="tilS"
                     /locus_tag="VFC15_01485"
     CDS             complement(421..2364)
                     /gene="tilS"
                     /locus_tag="VFC15_01485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZM08862.1"
                     /translation="MREKVLRYILERKLMRAGDRVAVAVSGGADSVALLRVLLELRTE
                     LGIVISVAHFNHGLRGETSEADEAFVAELARQYDLEFFCGRGDVRDHALTSKLSVEAA
                     ARELRYCWLTSLAETHKLDEIATAHTLDDQAETVLLKFLRGAGTRGLAGIYPEIAIGA
                     KAPDPFQPTEREPEEAVSRRHIGLPQCKEADKNQPRMGRKSLAQHGAAGGVLGKRDNR
                     PESRRDGRGYDTVSEAADPRIQSAERGTEAPLHRDNDPRHPGARIVRPLLEVTREEVE
                     LYLTALGQSWREDESNLDHRFARNRVRHVLLPLLEREYNPNIRQVLSDAAELSRAEEE
                     YWNVLVDWELEARQRTKTEIPPGLKPDGPVAGNGTAEAVPFQSSAAESCPTVSEPTSE
                     AAERTEGSDSADLQVRVPAPYICHPKPALAGGGSAFQAFSPGAEAVPRQTSASEQCLS
                     LVNFAELPLALQRRLLKRLLESQQIPADFQHIEKLLRCALGELPKAELPGGRLAVRQG
                     GCLELQAPQAEPPLSGYEYRLPVPGEVRIAELGLTLRSVPVPQEFANEAGPPESLLGA
                     ELLGPELVIRNWRPGDRFWPLHSKSEEKLKRLFSERHIPAEQRPSWPVVLSDPQIVWV
                     LGFPVGRAFAWSGSGDAVKIELL"
     gene            complement(2434..3393)
                     /locus_tag="VFC15_01490"
     CDS             complement(2434..3393)
                     /locus_tag="VFC15_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM08863.1"
                     /translation="MSHFLPVRNQNYQPEISHARAVLIIALVATIWGFGFPTTRIVLD
                     GGLSVGALMGLRFPLAGLVMFVIIRAKGIPITRRGVLDGIWLGLVLVVVFWLQTDGMR
                     FTTTAKAGFITGLYVLFTPLIAVAIGQRVKLASALGALIAVYGMYLLVHLRGAWWSGW
                     NRGDIEILLCAILCGVHIVMMGAFTRRSEAWLLAGSQVMICGVVSVGVTAFLPSPYGF
                     QNAFAALSHWPVAGAALYLALGSTVFAFWGQATAQTRLGPTESAVLFSLEPVVAAVLS
                     VVWLREPMSPRQAVGGALIVVAMIVAEALPYMFRAITVPDTRP"
     gene            complement(4467..4691)
                     /locus_tag="VFC15_01495"
     CDS             complement(4467..4691)
                     /locus_tag="VFC15_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM08864.1"
                     /translation="MEKVTVSSKGQIAIPKQVRDALNLKEGSKLTLEVHGQEIVLSKG
                     DAWRKLKGAAADMGDLMERFAAFRKEEREP"
     gene            complement(4743..>5504)
                     /locus_tag="VFC15_01500"
     CDS             complement(4743..>5504)
                     /locus_tag="VFC15_01500"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZM08865.1"
                     /translation="QIEDEVVGCIDFALAVLKTYGFEQFQVELSTWDPNDRAPYAGRD
                     DQWELAQRSLEQALKRRNIDYKLIPGEAAFYGPKIDIKLVDAIGRLWQLSTVQFDFTL
                     PERFGLEYVGEDGKRHQPLMVHRALYGSVERFFGVLIEHYAGAFPVWLSPVQVVVVPI
                     GEKHHAYAIEVGEKLKAAGVRVHVDVRNEKMNAKIREHAMQKVPFQLVVGDKEMEAGE
                     VNVRVRGQEKGEGSVKTEAFVERVKGLIENKAATL"
BASE COUNT         1116 a         1752 c         1543 g         1093 t
ORIGIN      
        1 gagaagctga tttcggcttc cttggcaccc gagccgccag ccttgacgac ctgccatagc
       61 aggacgcccg acaacacgat gaccagccag aacacgactg tttttaccgt cgaattcact
      121 gaaaactcct gtgtaacggg aaagacacaa aactctcccc aacactttag atgctgcccc
      181 tagacgccgg atgtacaaat tcggcgttct ctagattgta acccgcgcat aaacggaacg
      241 gcacgaaagg cttaaacaaa attctggaag ggcttgagcg gcaattccat tttggaagtt
      301 ccgtttatgg tcaacctgga gaagataacg cagggggatg tcacggagac cgcagtacgg
      361 tcgcaaggcg actgaattga gtcgaagccc gctggattag cggtgccccg tcgtcttcgt
      421 tcagagcagc tcgatcttca ccgcgtcgcc gctgccgctc cacgcgaaag ctcgccctac
      481 cggaaacccc agtacccaga cgatttgggg atcggacaac acgaccggcc aactcgggcg
      541 ctgctcggct ggaatgtgtc gctcggagaa caggcgcttc agcttctctt ccgacttgct
      601 gtgtaaaggc cagaagcgat cgcccggacg ccagttgcga atcaccaatt ccgggccgag
      661 caactccgcc ccaagcaggc tttcaggtgg gccagcctca ttcgcgaact cctgcggcac
      721 agggaccgac cgcaatgtca gccccagctc cgcaatgcgg acctcgcccg gaactggcaa
      781 tctgtactcg taacctgaga ggggtggttc ggcctggggg gcctgcaatt ccaggcaccc
      841 gccttggcgt actgccagcc gtccaccggg aagctccgcc ttcggcagct ccccgagggc
      901 acagcgcagc aacttctcga tgtgttggaa gtctgcaggg atttgctggc tctcaagcaa
      961 tcgcttcagc aaacgtcgtt gcagtgctag cggcaactcc gcgaagttca ccaaactgag
     1021 gcattgctct gacgcactgg tttgacgggg cacggcttca gcgcccgggg aaaaggcctg
     1081 aaaagcagat cccccgccgg ctaaagccgg cttgggatga caaatataag gagctgggac
     1141 gcggacctga aggtccgcac tatccgaacc ttctgttctt tcagcagcct ctgaagtcgg
     1201 ctcggagaca gtggggcatg actctgccgc actgctttga aagggcacgg cttcagccgt
     1261 gccgttgccc gctaccggac cgtcgggctt tagccccgga gggatttcgg tcttggtgcg
     1321 ttggcgggct tccagttccc agtccaccag cacgttccag tactcttctt cggcgcgcga
     1381 cagctcggcc gcgtcgctca acacctgccg gatgttcggg ttgtattcac gctccagcag
     1441 cggcagcagt acgtgtcgca cccggtttcg cgcgaaacga tggtccaggt tactctcgtc
     1501 ttcgcgccag ctctggccga gggcagtgag gtacagttcg acctcctcgc gcgtcacttc
     1561 cagcagggga cgaacgatgc gggcgccggg atggcgcgga tcattgtccc ggtggagcgg
     1621 ggcttcagtc ccgcgttccg ccgactggat acgaggatcc gcggcttcag agactgtgtc
     1681 gtaacctctt ccgtccctac gggactcggg tctattatcc cgtttaccca gcactccgcc
     1741 tgcggctccg tgctgggcta agctctttcg ccccattcgg ggctggttct tgtctgcttc
     1801 tttgcactgt ggcaagccga tatgacgtcg cgacacagcc tcttcaggct cgcgttccgt
     1861 tggctgaaaa ggatccgggg ctttagcccc gatcgcgatc tccgggtaaa tccccgccag
     1921 cccgcgagta cccgcgccgc gcagaaattt cagcagcacc gtttcggctt gatcgtcgag
     1981 cgtgtgcgcc gtcgcaatct catccagctt gtgcgtctcg gcaagtgaag tcagccagca
     2041 atagcgcagc tcccgcgccg ctgcctccac gctcaacttg ctagtgagcg cgtggtcgcg
     2101 cacatcgccg cgaccgcaga aaaactccag gtcatactga cgcgctaact ctgcgacaaa
     2161 ggcctcgtcg gcttccgagg tctctccccg caggccgtga ttaaagtgcg caaccgagat
     2221 gacgatgccc agctcggtgc gaagctctag cagcacgcgc agcagcgcga ccgaatccgc
     2281 gccgcccgac acagccacag ccacgcgatc gcccgcgcgc atcagcttcc gttccagaat
     2341 gtagcgaagc accttttcgc gcacggaatg atggtaatgc aggtgtcagc tttcagccat
     2401 tagctatcag ctatctgacg gctgacagct ccctcacggc ctcgtatccg gcaccgtaat
     2461 cgcgcggaac atatacggca gcgcttcggc tacgatcatg gccaccacga tcagcgcgcc
     2521 gcccacggcc tgacgcggcg acatcggctc tctcagccag accacgctga gcactgccgc
     2581 aaccaccggc tccaggctga acagtaccgc cgactccgtc gggccgagtc gcgtctgcgc
     2641 ggtcgcctgt ccccagaagg cgaagaccgt ggacccgagc gccaggtaga gtgccgcgcc
     2701 cgccaccggc cagtgcgaca gtgccgcgaa ggcattctga aacccgtagg gcgagggcag
     2761 aaacgctgtg accccgacgg acacgacgcc gcaaatcatc acctgcgagc ccgccagcag
     2821 ccacgcctcg gaacggcggg tgaatgcgcc catcatcacg atatggacgc cgcacaggat
     2881 ggcgcagagc aatatctcga tgtcgccccg gttccagcca ctccaccagg caccccgcag
     2941 atggacgagc aggtacattc cgtagaccgc gatcagcgca cccagcgccg aggccagctt
     3001 cacccgctga ccgatggcca cggcaatcag cggcgtaaac agcacgtaca ggccggtgat
     3061 gaagccagcc ttggcggtgg tggtgaaacg catgccgtcg gtctgcaacc agaagacaac
     3121 caccaggacg aggccgagcc agatgccatc cagcacgccg cgccgggtaa tgggaattcc
     3181 tttggcgcga atgatgacga acatcaccag tccggcaagg ggaaagcgca gccccatcag
     3241 cgcgcctacg gacagaccgc cgtcgagcac gatgcgtgtc gtggggaagc cgaatcccca
     3301 tatcgtcgcc actagggcga tgatgagtac ggcccgggcg tgggagattt cgggttggta
     3361 attctgattt cgcacgggca ggaagtggct caagttaaag gaaatcgcat ctttgcggtc
     3421 ttgtgcaact cacggtccca aagtgggagg actcgcctag cctcccgata catttcttct
     3481 tgacatttgg taattacatg tggcatcttc ttattaacag tagctattaa ccgaacttga
     3541 ccaaaagtaa taggaggtaa cgcaatgagt actaacttga gatggcgtcg gagatttcct
     3601 acctctgcga acaacttcga cttggttctg gcaaatccgc acgttttgga ctctgagcgg
     3661 ctaaacgaac ttctgaataa gattgagggg gagaaccacc gcagactcac tctgagttct
     3721 cggtctcgcc aggaacttcg acggttcttc aatcaaccgg atagccctct gaagagcttc
     3781 ggctatatcc tattaagcaa ttggtttatg acgcaaggcg gagatcggag cagtcagctt
     3841 gccagttgct gcgaagacct ttgggatgcg ctgtttccgt gtcgcccgcc cgaaaggttc
     3901 tcgtcgccag cagccggtca aaatcatgtg atgatgccgc tcgcattcga cgccttctgg
     3961 aaccgtgtct tgacggctca gggcgaggat attcctaaac ttgaccgagc ggcggcacct
     4021 ttaatcgtcg agcctacgct tgaaaatgga gacattcctg aatctaggga cagttcctcg
     4081 gaagatcgac tctgtttgga gcttgacaac ccacgattgc actgtaaggt cgagggctcg
     4141 gcccgcgcgc ttgctgactt ctggaggatc gtatcagcgt gggaagctca atgagaccgt
     4201 gggatcttta actgagaacg cggcgctgtt acgccagcgt cgcgctcttg ctttcaatca
     4261 gcgccttcac ccgctccatg aacgcctcgg ccttcacgga gccttggtcc ttctcctggc
     4321 acggacgcgc acgttcactt cgccggcatc catctcgttg tcaccgacga cgagccaacg
     4381 agccggagat cgccggagca cgatttacct atactgccgc accgccgatc cggtccacca
     4441 cggcccacga atcaaggacg cgagtcttaa ggttcgcgct cctccttccg gaaggctgcg
     4501 aacctttcca tcaaatcgcc catatcggcg gcggcgccct taagctttct ccaggcatca
     4561 cccttcgata gcacaatttc ctgcccgtgc acttccagcg tcagcttgga gccttctttg
     4621 aggttgaggg cgtcacgaac ctgtttcggt atagcgatct gtcctttaga ggaaaccgta
     4681 accttttcca ttaattcatc ttaccgcagg taagccttcc tgaagcctag caatgcgcga
     4741 aatcaaagcg tcgccgcctt gttctcgatc agccccttca cccgctccac gaacgcctcc
     4801 gtcttcaccg atccttcgcc cttctcttgc ccgcggacgc gcacgttgac ctcgccggct
     4861 tccatctcct tgtcgccgac gacgagttgg aacggcacct tctgcatggc atgctcgcgg
     4921 atcttggcgt tcatcttctc gttgcgcaca tcaacgtgca cacgaacgcc ggcagccttc
     4981 aacttttctc cgacttcgat cgcgtatgcg tggtgcttct cgccgatcgg caccacgacc
     5041 acctgcaccg gcgacagcca cacaggaaac gctcccgcgt agtgctcgat gagcacgccg
     5101 aagaagcgct cgaccgagcc gtagagcgcg cgatgcacca tgagtggctg gtggcgtttg
     5161 ccgtcttcgc caacgtactc cagcccgaag cgctcgggca gggtgaagtc gaactgcacg
     5221 gtcgagagtt gccacaggcg gccaatggcg tccaccagct tgatgtcgat cttgggccca
     5281 tagaatgcgg cctcgccggg gatcagcttg tagtcgatgt tgcgccgctt cagcgcctgt
     5341 tccagcgagc gctgcgctaa ttcccactgg tcgtcgcggc cggcgtaagg cgcacgatcg
     5401 ttcgggtccc aggtggacag ttccacctga aactgctcga agccgtaggt cttcagcacg
     5461 gctagtgcga aatcgatgca gccgaccacc tcatcttcaa tctg
//
LOCUS       DASRKK010000072         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_785995, whole genome shotgun sequence.
ACCESSION   DASRKK010000072 DASRKK010000000
VERSION     DASRKK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785995"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1373)
                     /locus_tag="VFC15_01505"
     CDS             complement(69..1373)
                     /locus_tag="VFC15_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZM08866.1"
                     /translation="MSQTTIDRFARLKALQRPSVLPLSGHAVGPVENPVVGEAIDSAS
                     GSVIGAERLALILGATVKRNGHGEHLSLHCWHGGHAACDPDAAALRLLAPNAPEDIAD
                     AQQWLFLDTETTGLAGGTGTYPFLVGLAWWEGGGLEVEQLFMREYSEERSLLVALAER
                     LAERPVLVTFNGKSFDWPLLETRYRMMRTIPPPVPRAHLDFLHPARNLWRLRLGSVRL
                     SQLERYVLGWDRGADIASELIPRIYLDFVRGGRAEPLVPVFQHNQMDLRGLAGLSGRI
                     LSLLGDKEGTSQDGLELYGVSRICERRGEAKRARRLYEQLIASALPEETDRVARAALA
                     RLAKRDGDFARAHELWESVLGNSREGYEAYEQLAIYYEHEAREPRRALALAREALDQL
                     RRANQVGTVAATEYRRTKGRFEHRMLRLERKASRTLLDTFGA"
     gene            complement(1420..>2773)
                     /locus_tag="VFC15_01510"
     CDS             complement(1420..>2773)
                     /locus_tag="VFC15_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HZM08867.1"
                     /translation="GYLPGERREIERGLRDGRIRGVVATNALELGIDIGSLDACVMAG
                     YAGSIASTWQRAGRAGRRSGSSCAVFVASSAPLDQFIVQHPEYFFGSSPEHAHIQPDN
                     LEILVNHLKCAAFELPLSPDERFGQVELPELCERLAEAGFLQRSGGNWHWVQESYPAD
                     TISLRSVTSDNFVIIDTTGGEDGKPEVIGEVDFSSALTTVHPKAIYIHQGQQYHVERL
                     DFGQRKAYVKRVDVDYFTDAIRYTQVRVLEIAEEQSAAGPAARAHGDVLVRSQVVGFK
                     KIKFFTNENVGSGKLELPENEMHTTAFWITLQHELLAGLPFTLSERQSGIFGLIYALA
                     SVATLLLMCDRRDIGTAVGERPPHGGADVEWQEFATTPGNEKGLFEPNLYLYDAFPGG
                     IGFSEPLYRVCQVLLARTQELITACPCDNGCPSCIGPAGDQSERSKEVALAILGRLCS
                     "
BASE COUNT          544 a          898 c          795 g          536 t
ORIGIN      
        1 ccgtggttag ggtcaatccc agaagatcgg cgatctccgg cgcggtcaag gagtgggcca
       61 atccgaggct atgcgccaaa cgtgtcgagc agcgtccggc tggccttgcg ttcgagacgc
      121 aacatccgat gctcgaaccg ccccttagtt cggcgatatt cagtcgccgc gactgtccct
      181 acttggttcg cacgacggag ctggtcgagc gcttcacgcg cgagcgctag cgcgcgtcgc
      241 ggctcgcgcg cttcgtgttc atagtagatt gccagctgtt cataagcttc gtagccctcg
      301 cgcgagttcc ccagcacact ttcccagagt tcgtgcgcgc gtgcgaagtc cccgtcgcgc
      361 tttgcgagcc gcgcgagtgc cgcgcgggcg acacgatcgg tttcctccgg cagagcggag
      421 gcgatcaact gttcgtacag tctccgcgca cgctttgcct cgccacgtcg ttcgcaaata
      481 cgagacaccc cgtacagctc taatccatcc tgactagttc cctccttatc gccgaggagt
      541 gaaaggatgc gacccgagag tccagccagt ccgcggagat ccatctggtt gtgctggaag
      601 acagggacca acggctctgc gcgtcccccg cgcacgaagt cgagatagat tcttggaatc
      661 agctccgagg cgatatctgc tcctcggtcc catcccagca cataccgttc gagttgcgac
      721 agtcttaccg agccaagccg cagccgccac agattccgtg ctggatgaag aaagtcgagg
      781 tgggctcgcg gtaccggcgg aggaatggtt cgcatcatcc ggtagcgtgt ttcgagcagt
      841 ggccagtcga aggacttgcc gttgaacgta accagcaccg gacgttcagc taaccgctcg
      901 gcaagcgcca cgagcagcga acgttcctcg ctgtactcgc gcatgaacaa ctgctcaacc
      961 tcgaggccac cgccttccca ccaagcaaga ccgaccaaaa acggatacgt tcccgtcccg
     1021 ccggcgagtc ctgttgtctc cgtatcgaga aagagccact gctgagcgtc ggcgatatcc
     1081 tcaggcgcat tcggtgcgag caaccgcagt gccgcagcat ctggatcgca cgcggcgtgg
     1141 ccgccatgcc aacagtgcag tgacagatgc tcgccatggc cgttccgctt taccgtggcc
     1201 cccagaatca gcgctagcct ctccgcccca atgaccgagc cagaagcgga gtcaatcgct
     1261 tctcccacga ccgggttctc caccggcccg accgcgtgtc ctgacaaagg caggactgag
     1321 gggcgctgca acgcctttag gcgggcaaag cgatctattg tcgtctgact cacggtacgt
     1381 tgaataccta ccgttcttct tattcggcgt tgccgcctct caggaacaca acctgccgag
     1441 gatcgccaga gctacttcct tggaacgctc gctttggtca cctgcggggc cgatacacga
     1501 cgggcatccg ttgtcacatg gacaggctgt tatcagctcc tgcgtgcgcg ccaacagcac
     1561 ttggcagacc cgataaagcg gttcgctgaa tccaatccct ccgggaaacg cgtcatacag
     1621 gtacaagttc ggctcgaaga gtcccttctc gttgccgggc gtagtggcga actcctgcca
     1681 ttccacgtcg gcgcccccat gcggtggccg ctcgccgaca gcggtgccaa tgtcccggcg
     1741 gtcgcacatc agcagaaggg tcgcgaccga ggcgagcgcg tagatcaacc cgaagatgcc
     1801 gctctgccgc tccgaaaggg tgaaaggcag tcccgccagc agttcatgct ggagcgtgat
     1861 ccagaacgcc gtcgtgtgca tctcgttctc gggcagctcc agctttccgg agcccacgtt
     1921 ctcattggtg aagaacttga ttttcttgaa accaacgacc tgcgaccgca ccagcacgtc
     1981 gccgtgcgct cgcgctgccg ggccggcggc actctgctcc tcggcaattt cgagcacccg
     2041 cacctgggtg tagcggatgg cgtcggtgaa gtagtccacg tccacgcgct tcacgtaggc
     2101 cttgcgctgg ccaaaatcaa gccgctcgac gtggtactgc tgcccctgat gaatgtagat
     2161 cgccttggga tggacggttg tgagagcgga cgaaaaatcc acttcaccaa tcacctccgg
     2221 ctttccgtct tctccgcctg tcgtatcaat gatgacgaaa ttgtcggatg ttaccgaacg
     2281 caggctgatc gtgtctgccg gatagctctc ctggacccag tgccagttgc ccccgctacg
     2341 ctgcaggaac cctgcttccg caagtcgttc acagagctcc ggcaattcca cctggccgaa
     2401 tcgctcgtcg ggcgagagcg gcaactcgaa tgccgcacac ttcaaatggt tcacgagaat
     2461 ctcgaggttg tcgggctgaa tgtgggcgtg ctcgggtgag cttccaaaga agtattcagg
     2521 gtgctgcaca ataaattggt cgagcggagc agaagacgca acgaacactg cacacgagga
     2581 accgctgcgg cgccccgcac gcccggcgcg ctgccaggtg gacgcaatcg atcctgcgta
     2641 acccgccatc acgcaagcgt ccagagatcc aatatcaatt cccagttcaa gagcgttggt
     2701 tgccacgact ccgcggattc ggccatcgcg cagcccacgt tcgatttcgc gacgttcccc
     2761 gggtagatat ccg
//
LOCUS       DASRKK010000073         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_590421, whole genome shotgun sequence.
ACCESSION   DASRKK010000073 DASRKK010000000
VERSION     DASRKK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590421"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFC15_01515"
     CDS             <1..201
                     /locus_tag="VFC15_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08868.1"
                     /translation="GNETALATEVTETLPQALKRDPRIDGSYARLKACSTHRLYTRCE
                     TAIKYHDVLTKANESYIGLPVG"
     gene            complement(541..1092)
                     /locus_tag="VFC15_01520"
     CDS             complement(541..1092)
                     /locus_tag="VFC15_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08869.1"
                     /translation="MAGLYRHVIYGVLLSSVLLTHTTSAGAQSAAASGASGTPHVSIS
                     PQTAALERDFFAAIREEDTKKFLSYVPEEGIHVGPQAKLVSRAEVAEQLSHRRELYCK
                     LFDSSCIDAPIKLDASSRACSDRELLTHSEKVRTAASETVRNAVRQAILVAEVKNKRC
                     GGTNLVDFIFNYVQGEWKLFSAP"
     gene            complement(1098..1547)
                     /locus_tag="VFC15_01525"
     CDS             complement(1098..1547)
                     /locus_tag="VFC15_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZM08870.1"
                     /translation="MDIEAIRKYCLQFPHTTEIVQWGADLCFKVDGKLFVVAAMEMVP
                     QRVSFKCTPENFAELCERPGIIPAPYMARAQWVSLERLNALPDSELREMISESYRLVW
                     ERLPMKRRVELESGAAAGARQPAVRRPKNASRKSSVKTKNAKAGRRG"
BASE COUNT          420 a          645 c          639 g          438 t
ORIGIN      
        1 ggcaatgaga cggctttagc cactgaggtg acggaaacat tacctcaggc gctaaagcgc
       61 gatccaagaa ttgacggcag ttatgcacgc ctgaaggcgt gctccaccca tcgcctatac
      121 accagatgtg aaactgctat aaagtatcac gatgtcctga cgaaggctaa cgagagctat
      181 atcggtctgc cggttgggta atcccgtcgc cctgaagggc cgaagcgtca atgttagact
      241 ttagtgctac atttgtttaa ttagtgtaaa acttacgtga caactatcaa gaccaccatt
      301 tgggtatcat gatggcctcc tataatgcca gttaggagcg ctggcgatcc cgtaggcagc
      361 gttttacggc tgcggacgac ccaaagtacc tccggagcgg ctacggtcgc tacaagcgcc
      421 tctgaacgcg aaatccggag tcggcgcaat tctggcgcat ttcccgattt gggccggatc
      481 gcaaccttcc gctagagcct gcgaggcgcc tcacctgacc tttttcagca gctccatagt
      541 ctagggtgcc gagaacagct tccactcgcc ctgcacgtaa ttgaagatga aatcgacaag
      601 gttcgtgccg ccgcaccgct tgttcttgac ctcggccacc aggatcgcct ggcgcacggc
      661 gttgcgcacc gtctcgctgg cggcggtgcg gactttttcc gagtgcgtga gcagctcgcg
      721 gtcggagcag gcgcgcgagc tggcgtccag cttgatgggg gcgtcgatac aggaagagtc
      781 gaacagcttg cagtacagct cgcgccgatg cgacaactgc tctgcgactt cggcacggct
      841 caccagcttt gcttgcggac caacatggat accctcctcg ggcacgtagg acaagaactt
      901 cttagtgtcc tcttcgcgaa tggcggcaaa gaagtcgcgc tccagtgcgg cggtttgcgg
      961 cgagatcgaa acatgcggcg tcccggatgc gcccgatgcg gcagctgact gggctccggc
     1021 ggaggtcgtg tgcgtcaaca acaccgacga caacagcacg ccataaatga catgacgata
     1081 caggccagcc atccaattca tccgcgccgg cctgccttcg cattcttggt tttgaccgat
     1141 gatttgcgag acgcattctt cggcctgcga actgccggtt gtcgtgcgcc tgccgccgcg
     1201 ccgctctcca gctcaacccg cctcttcatt ggaaggcgtt cccataccag ccgataggat
     1261 tcggaaatca tttcgcgcag ctcggagtcg ggcagggcgt tcagcctttc gagactgacc
     1321 cactgcgcgc gcgccatgta gggcgcggga atgattcctg ggcgctcgca cagctcagcg
     1381 aagttctcag gcgtgcactt gaacgaaacc cgttgcggca ccatctccat cgccgctact
     1441 acgaacagct tgccatccac cttgaagcac aggtccgctc cccactgcac aatttccgtt
     1501 gtgtggggga attgaaggca gtacttccga atcgcttcaa tgtccactgt ctcctccgtt
     1561 tacgcgaggt gcgagctcgg gccactcgcg catcatgacc agcttggtgt acgccaggcg
     1621 aaaaccccgg cgttccatat tacgttgcga accgctggcg ggctgcgtgc tcaccacagc
     1681 atactcgcag cctgcgaccg cagcttcatg caggcggcgc tcgattagcg cgctgtgtac
     1741 gccccggttc cggaaggctg gcagagtcgc ggtaccgtac agagcggcga tccgcgcttc
     1801 gggtatgatg cggccgccac agccgcggcc attgggtcgg attcacggaa agcccgctac
     1861 gcggctactg ggcggtgacg atcattgccg ggtggccgac ggtccgggag ttatcggcga
     1921 tgaggcgctc aatttgcgcg atgcgttcct cttcggtcgg gtgcgagcgg aaatatcctt
     1981 ccagcgtctg gaggaggacg acgttggcaa tgtccgccgg tccagaactt cttgccggtt
     2041 acgggccggc ctgcgcaacg ctcgcgcgaa ggggaagaag ttcggaaggc cccgttgaag
     2101 tgcgctccgt cgaaaactgc gtcctgattg ggagtcgggc tg
//
LOCUS       DASRKK010000074         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_395175, whole genome shotgun sequence.
ACCESSION   DASRKK010000074 DASRKK010000000
VERSION     DASRKK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395175"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFC15_01530"
     CDS             <1..235
                     /locus_tag="VFC15_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08871.1"
                     /translation="RRFPVRPNTREPNPENTIKGRQLESPFLVPALENEKLMAQGEDF
                     CLQSDSRTERITKSGEQANQDREHHPEPIAILR"
     gene            complement(1382..>2015)
                     /locus_tag="VFC15_01535"
     CDS             complement(1382..>2015)
                     /locus_tag="VFC15_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM08872.1"
                     /translation="SETTVSRWLRRAPRPPDVAKRWLTFLRNHREAIAAMDFFTVPTF
                     TFGILYCFFVISHDRRKILHCSVTRQPNAFWIVLQLREAWGFEPPPRFLIFDRNSKFS
                     ADVVTTVEDNGTQPVRTAFRSPWQNGVAERWVGSVRRDLLDHVIVLNERHLRRLLKDY
                     VRYYHEDRTHLGLGKDTPGDRVAASAPPRGQKVISLPRLGGLHHRYAVAA"
BASE COUNT          486 a          544 c          576 g          409 t
ORIGIN      
        1 gcgccgattt ccagtccggc caaatacgcg agagccaaac ccagagaaca cgatcaaagg
       61 ccgacaactg gagtcgccgt ttctggttcc ggcgctggag aacgagaagt tgatggcgca
      121 aggcgaggac ttctgcctgc agtccgactc gcgtacggag cgaatcacga agagtggcga
      181 gcaagcaaac caagaccggg agcatcaccc agagcctatt gccatattgc gctaagtgca
      241 atccgttcag cgagaatgag tttttggtag gcagcggtga tttcaaggtg agtgaagaag
      301 aactggttga gatcgaagcc gctcatgagt gcgatataac acgcaggtcg agacagttct
      361 aaaatttcaa actgcgccca ctaccttctg ggcgctgtga tttttggtag tggtgcagtt
      421 tgaaatcgac agggcttgaa cggctggagg aacggcgtaa actgtgggca tggcaaaagc
      481 atacagaaca gatctcctta gcgaaaaacg aatctgcgat gctatcgcat cactggggaa
      541 cgtagaaggg ccggagcgcc tttcatgccc cgtggacggc tgtgcgtgca gttacgaagt
      601 atacgaatac gtgctctctc acacgaagag tcaccgggaa aacctacaag ataagctcag
      661 gagggaacac cctaaccacc ccgaggagat tttcgtactg aatgagctgc agccggagaa
      721 agtggctcag gaaaatttca gcccagccga agcgaagaag gcgtagacat ggcaaaagag
      781 acactcccgc cgcatttgtt tgccgatggc gatctaaaac gctgctcggt ctgcgggcac
      841 ccgttcgacc ctgacgtgaa gccgtccatg agcgtggcat tctcagatca cctgagcaag
      901 gcccacaggc ccggctaaac gagcgaggac ttcagccaag ctgctgtgcg ggtcgtgaaa
      961 gaagcgaccg agaaggttta atcgcgtttg accgtaacga ccagcggcaa tcgctcctgg
     1021 tattccttgg cgaatgcgcg tttgaaatta tcctcgaagt ccttcttgtc atctgacact
     1081 ttcataatca ttgtaacggc tgtgatctgc cggtcgagat gcacgtttcc agtatcggcg
     1141 gttagaaact gaaaatgctt gtgcttccga taccccttct cctgtgcctc gtcagaacta
     1201 aacgcagcac cgtttcgcct acgatagctc cttgcgtgga tcgcacctgc cttgagtcgt
     1261 gcggtcttcg acggctgtga ctaccctaag aacgagcggg agcactcgcc tgacaggatc
     1321 caaacataaa tctccttccg gccctgattg cgaacaactc taggccatag ccgcgggtgg
     1381 cttaggccgc gaccgcgtag cggtgatgca gaccaccgag tcgcggcaag gagataacct
     1441 tctgtcccct cggaggcgcg gacgcagcga ctcgatcccc aggcgtgtct ttcccaagtc
     1501 cgaggtgagt ccggtcctcg tggtaataac gaacgtagtc ttttagcaac cgtctcaggt
     1561 gccgctcatt gagaacgatc acgtggtcga gcaagtctcg acgtacgctc ccgacccagc
     1621 gctccgccac cccgttctgc caaggactgc gaaaggcggt gcgcaccggc tgagtgccat
     1681 tgtcttccac ggtcgtaacg acatcggcac tgaatttcga attacgatcg aagatgagga
     1741 atcggggtgg tggttcgaaa ccccaagctt cgcgcagctg gaggacgatc cagaaggcat
     1801 tcggctgccg agttacgctg cagtgcagga tcttccgccg gtcgtgacta atcacaaaga
     1861 agcaatacag aatgccgaat gtgaatgtgg gcacggtgaa gaagtccatc gctgcaatgg
     1921 cctcgcgatg gtttcgcagg aatgtcagcc aacgcttggc gacatctgga ggtctgggcg
     1981 ctcgccgaag ccatcgtgag accgtcgttt ccgat
//
LOCUS       DASRKK010000075         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_786202, whole genome shotgun sequence.
ACCESSION   DASRKK010000075 DASRKK010000000
VERSION     DASRKK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786202"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..807
                     /locus_tag="VFC15_01540"
                     /pseudo
     CDS             58..807
                     /locus_tag="VFC15_01540"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
     gene            complement(834..1529)
                     /locus_tag="VFC15_01545"
     CDS             complement(834..1529)
                     /locus_tag="VFC15_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08873.1"
                     /translation="MLRSKPEVDAIELSALREQELIPLIEALPSLDLRHFRHVSFHAP
                     SAMSGDFEAVATKRLEEVASRKWLIVVHPDVITKPDLWKCFGEQLCVENMDKRKPIGQ
                     TADQLQEVFDKLPSSSFCFDLGHARQVDPTMTEAFLILRRFRGRLRQLHVSEVNSDSR
                     HGELTLEASLAFRRLASVIPNDVPAIIESRVPKPDIDREVRLVREMFDGVTSEGISTG
                     GTSALHPVGVPVT"
     gene            complement(1592..2668)
                     /locus_tag="VFC15_01550"
     CDS             complement(1592..2668)
                     /locus_tag="VFC15_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZM08874.1"
                     /translation="METGPLPTLFSQLGIKCSHIDAASKQARETRAAIEAALSRNLAQ
                     WVSLDTSLVVFGSLARNEFTRKSDVDWTYLIDGQANSDHLTISREISEVLRGAGFKGP
                     NPQGAFASMVFSHDLIHQIGGQEDTNRNMTQRMLLLLESVAIGERAQEAYERVMKGVI
                     SRYLEEGSRLLTSDGGRYKVPRFLLNDVARFWRTMAVDFASKQRDRGGTGWGLRNLKL
                     RMSRKLLFASGLLTCFSPCLDPEAKPLESAGSDELQAHLIQYLRDQFRLSPLEVLGNA
                     IRFCNVPNDAGIRLFESYDEFLGMLDDDNIRDKLEKLRAENASGDSTFKRVREISRQF
                     QAALDSIFFENKLIWPLTKKYGVF"
     gene            complement(2722..>3448)
                     /locus_tag="VFC15_01555"
     CDS             complement(2722..>3448)
                     /locus_tag="VFC15_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZM08875.1"
                     /translation="PTESNQPYDIKDVINAVVDDSYFFEVQEHYAKNIVIGFARLNGK
                     PVGIVANQPAFLAGVLDIKASIKGARFVRFCDCFNIPLITFEDVPGFLPGTQQEYGGI
                     ITHGAKLLYAFSEATVPKITVITRKAYGGAYCVMASKHIRTDANYAWPTAEIAVMGPE
                     GAVNIVYRHELEKSENPEADRKAKIEEFRERFANPYIAAERGYLDAVIRPRDTRRKLI
                     QALDMLENKRAKNPPKKHGNIPL"
BASE COUNT          756 a          997 c          925 g          770 t
ORIGIN      
        1 cccatcgcgc aggtcgaagg ctcgtccatg cagcgcgact actggtattc ggtcgcgatg
       61 acgctgaaga agctggagtc gccggaagaa gtcggcaaga ttgccgcaca gcgcaccttg
      121 cggcgcctcg gagcgcgcaa ggtcaagacc gcaaaagttc ccatcgtgtt cgagcgcacc
      181 gtcgccggag cgctggtcgg ccacatcttc gaggccgtca acggcgactc gatctaccgt
      241 ggcgcgtcgt tcctcaccgg caagctcaac gagaagattg caggcgacaa catcaacgtg
      301 gtggacgacg gcaccatgcg cggcggcttc ggcaccagcc cattcgactc ggaaggtgtg
      361 cccagccgca agactgtcgt agtcgaaaac ggcgtgctca agtcgtacct gctgaatacc
      421 tacacggcga aaaagctgaa catgcagact accggcaacg cttcgcgcgg gctggcggga
      481 acgccgggca tcggtccggg caacttcttc ctccaaccgg gcgcgaagag cctacaggag
      541 atcattggcg acatcaagga aggtctgttc gtcacctaat tcctcggctt cggtgtgaac
      601 ctggtgaccg gcgacttctc gcgcggcgcc agcggtctgt ggatcgagaa cggcgaactc
      661 acctatccgg tggaagagat caccgtcgcc ggcaacttga aggacatgtt cttcaacatc
      721 gcggagatcg gcaacgacct cgaattccgc ggcgccatcg cgtcgcccac gcttcggatt
      781 gacggcatga cggtggcggg ggaataagtc ctgatccgcc gaccgtgtgt ggattaggtt
      841 acgggcacgc cgaccgggtg cagagcgctc gtcccgccag tcgagatgcc ctcgcttgtt
      901 actccatcga acatctctct taccaacctc acttctctat cgatatcggg tttgggtacg
      961 cgactttcga tgatcgcagg aacgtcgttg ggaatgaccg aagcaagtct cctgaatgcc
     1021 aacgacgcct caagagttag ctctccatgc cggctgtctg aattcacttc gctgacgtga
     1081 agttgcctga gcctgcctcg aaaccggcgc agaatcagga acgcctccgt catcgttggg
     1141 tcgacttgcc tcgcatggcc caaatcaaag cagaacgagg aagagggtaa cttgtcgaag
     1201 acttcctgaa gttggtcggc tgtttggccg attggcttac gtttgtccat attttccaca
     1261 cagagctgct ctccgaagca cttccacaaa tcgggttttg taattacgtc tggatgaaca
     1321 actatcagcc actttctgct agcaacttcc tctaatctct tcgtcgctac tgcctcaaag
     1381 tcgccactca tggcactggg agcatggaaa gaaacgtgcc tgaaatgccg caagtcgaga
     1441 ctcggaagcg cctctatgag aggaatcaac tcctgctccc gcaatgcaga gagctcaatc
     1501 gcatcgacct caggcttgct tcgcagcata gtcaacgcgc aacggaagtc tcctagtgcc
     1561 aaagctcccg ttgaaaaccc aatcgatctc atcaaaagac cccgtatttc tttgtgagcg
     1621 gccaaattag tttgttttca aagaatattg aatcgagggc agcctgaaac tgacggctta
     1681 tctctcgaac tcgcttaaat gttgagtccc ctgatgcatt ctccgcccga agtttttcga
     1741 gtttgtcacg aatattatcg tcatcgagca tccctaagaa ctcatcgtac gattcaaaga
     1801 gtctaatgcc cgcatcatta ggtacattac agaaacgtat tgcatttcca agcacttcaa
     1861 gagggctcag gcgaaactga tctctcaggt attgaatcag gtgggcttgc aattcgtcac
     1921 ttccggcgga ctcgagtggc ttcgcttctg gatccaaaca tgggctaaag caggttagga
     1981 ggcccgaagc gaacagcagt ttgcgagaca tgcgcaattt caaatttcgg aggccccagc
     2041 ctgtgcctcc ccgatcgcgc tgcttgctgg caaagtccac cgccatcgtc cgccaaaatc
     2101 gagcaacgtc attaagtagg aatcgtggaa ctttatatcg acctccatcc gaagtcaaaa
     2161 gcctcgatcc ctcctcgagg tatctgctta tgaccccttt catgactctc tcgtaagcct
     2221 cttgggctct ctctccgatc gcgacggatt ccaacagcaa cagcattcgc tgcgtcatgt
     2281 tccgattggt atcctcttgg ccgccaattt ggtggattaa gtcgtgacta aaaaccatgc
     2341 ttgcgaatgc gccttgggga tttggaccct tgaatccggc gcctcgcagg acctcagaga
     2401 tttctcttga aatcgtgaga tgatctgagt tcgcttgacc atcgatcaga taggtccagt
     2461 cgacatcact tttccgagtg aactcattgc gagccaacga gccgaagaca accaggctgg
     2521 tatcgaggga gacccactga gcgagattcc gcgatagcgc agcctcaata gccgctcttg
     2581 tttcacgagc ctgcttgcta gcggcatcaa tatgggaaca cttgatgccc agttggctaa
     2641 aaagagtggg taagggacct gtttccatga atcatcgatt ctagaccaga tcataccctc
     2701 aaatatccac agcctcaatt tttacaacgg tatattcccg tgcttcttgg gtgggttctt
     2761 ggcgcgcttg ttctccagca tatcgagcgc ctgaatcagc ttgcggcgcg tatcgcgcgg
     2821 acgaatcacc gcgtcgaggt agccgcgctc ggccgcgatg taaggattcg cgaagcgctc
     2881 ccggaactcc tcgatcttcg ccttgcgatc ggcttccgga ttctccgact tctccagctc
     2941 atgccggtac acgatgttca ccgcgccttc gggtcccatc accgcgatct cagcggtcgg
     3001 ccaagcgtag ttcgcgtcgg tgcggatgtg cttggacgcc atgacgcaat aggccccccc
     3061 ataagctttg cgggtgatca cggtaatctt gggcacggtc gcctcggaga acgcgtagag
     3121 cagcttcgcg ccgtgggtga tgatgccgcc gtactcctgc tgcgtgcccg gtagaaatcc
     3181 tggaacgtct tcgaaggtga tgagcggaat gttgaagcag tcgcagaagc gcacaaagcg
     3241 cgctcccttg atgctcgctt tgatgtcgag cacgcccgcc agaaacgcgg gctggttggc
     3301 gacgatgccc accggcttgc cgttcagccg ggcaaagccg atgacgatgt tcttcgcgta
     3361 atgctcctgc acctcgaaga agtagctgtc gtccaccacg gcgttgatca cgtccttgat
     3421 gtcgtagggc tggttcgatt cggtagga
//
LOCUS       DASRKK010000076         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_199698, whole genome shotgun sequence.
ACCESSION   DASRKK010000076 DASRKK010000000
VERSION     DASRKK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199698"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(540..746)
                     /locus_tag="VFC15_01560"
     CDS             complement(540..746)
                     /locus_tag="VFC15_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08876.1"
                     /translation="MLLHTQPYQRDLTKFERLSAQEALRVKERAKLLPEGRERERLSH
                     EARQLEIASHINAWISSPGLRPPR"
     gene            complement(1227..2317)
                     /locus_tag="VFC15_01565"
                     /pseudo
     CDS             complement(1227..2317)
                     /locus_tag="VFC15_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612134.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          477 a          721 c          673 g          523 t
ORIGIN      
        1 gtcgagagtg cgacaatcaa tcagtaatcg atgtgtctaa gcttccgtgt gtcgatgtca
       61 tcgcgaatca ctttcaatta gataacgcat tggcgacccg aagatatatc tgccgttttg
      121 gaactatcga ggtttcgacg agtagtgact ccgagcgaac acggcctgcc agcgccgctg
      181 tcatacccaa cgcttatacg cctcttcaga accatgagca ggcatttccc ggagcaatac
      241 aatgaagacc aaattcttcg tactcggagc aatcgcagca agggtaagcc taggcctgat
      301 ctaccagccc caactactgc gacgacttcc gtgttcgatc cgcgttggcc tctagccaag
      361 cctcgttcac aaacagcgat cgtagcgatg atgcaatctg gcgatggtag ttgcgttcgg
      421 gtccgggcgg aaacgagcgg gcgtggagca acgtgcctcg ccgcactttg gtcaactctg
      481 ccaaactgta gactcggtgg tctggcgtcg attgagttgc ctgaggcatg atgatctccc
      541 tatctcggtg gccggagtcc gggagacgat atccacgcat tgatgtgcga agcgatctca
      601 agttgcctgg cctcgtggga tagacgttcg cgctcccgcc cctccggcag aagtttggct
      661 cgctctttga cgcggagcgc ttcctgggcc gagagccgct cgaacttggt gagatcgcgt
      721 tggtagggct gggtatgcaa cagcacagct aagctctcct gagttaatag gcgggagggt
      781 gcagcactct ctgtcacaga tgaatgccga taagcggcgg cgatggcggc atcatcgcac
      841 ctccggtttc ggacgtgctg gtcaatatag ctcacatcgg aaaaatctaa cgaaaaacgc
      901 taaagctttc gtagtaaggc gttgcagagt ctattaagtc ccggttctgt ggcgtcgcgc
      961 ctcaatggcg catttgctcg ctccgcattg aaagtcgcag cgaactgtga agtggacccc
     1021 ggagttggga ccagggatcg ccggagttaa ggtgtaatcg cgcctgcctt tgtccttcaa
     1081 gatcaagcag cttcctgctg ctgcgtttgc gtctgctgtg ggtatgtggg caacgcgttt
     1141 gcgttgtcca agcgaagcgg catatccaca gtcttcgcca cctcgatcct gtccatgccg
     1201 gcacgccatg ccaccatggg aaactggttg ttcatcgcct gatgcgggcg acgatgattg
     1261 tagaagttca tccacgatcc gatgccgtct cgcgcttcgc gcccatcggc ataggctttc
     1321 agatgcactt cctcgtattt gatcgagcgc cacagccgtt cgatgaaaat gttgtccatg
     1381 aaacgaccgc gcccgtccat cgaaatcgcg atgcctgcag cctcgagcct gccggtgaag
     1441 gcggcgctgg tgaactgcgc gccctgatcg gtgttgaata ttttaggccg gccgtgccgc
     1501 gccatagctt cgtcgagcgc cgcgatgcaa aagcccgtat ccatcgtgtt cgacaagcgc
     1561 cacgccagaa ccgcccggct ggcccagtcg atgatcgcca ccagatacag aaagccctgc
     1621 gccatcggga tataggtgat gtcgctggcc cagacgtgat tgggctcggt gatgctcacc
     1681 ccgcgcagca gatagggata gatcttgtgg cccggcgcgg ctttgctggt gccgggacgc
     1741 gggaccagcg cctcgatccc catcacccgc atgagccttt gcacgcgctt gcggttgatg
     1801 ccgtggcctg cctggttgag ttcgaacacc attcgccgcg agccgtaaaa cggccacttc
     1861 agatgcagtt cgtcgatccg tcgcatcaag gccagatcgt cggtgctggt caccggcccg
     1921 ggccgataga cgcccgaacg tgccagattc aacagctcac attgccggcg aaccgacaaa
     1981 ttctcgccgg gccgctcgac catcgccctg cgctcgggaa cactcatcgc ccgggcctct
     2041 tggccaagaa atccctttca actgtcaact ggccaatctt ggtataaagc ttggccgtct
     2101 cgcgctcatg ctcctccgcg ccatccccta aggcattcgc gccgcgcgcg aacaggctcg
     2161 tcaggttgtc cagaacctgc ttcttccagc catagatcag gttcgggtgg acgccgtgcc
     2221 gcttcgccag ctccggcacc gtggcaacct cgcgcagtgc ctccagagca atcttggcct
     2281 tgaacgcggc atcaaacttc ttgcgtgact tagtcatatc cgtaccccat cggttgagac
     2341 agagcaggac gcttcacacc ttaacatctg gtcctatttc cggggtccac ttca
//
LOCUS       DASRKK010000077        10988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_4409, whole genome shotgun sequence.
ACCESSION   DASRKK010000077 DASRKK010000000
VERSION     DASRKK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4409"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2266
                     /locus_tag="VFC15_01570"
     CDS             95..2266
                     /locus_tag="VFC15_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM08877.1"
                     /translation="MLRVLLVVLILVPMFANSQNSTTAAQPNSPSQPQGTAVKPPVAK
                     IVHTERTVNGKQLVDDYAWLRERSNPEVKALLEAENAYAEFVMKPTEARQKKLYDEIV
                     SHIKESDDTVPFLKDGYFYYTRTEKGKQYYQLCRKKGSLDAPEQIILDLNQMGEGQKF
                     MAVGQWSVSDDGNLLAYTTDNVGFRQYHLHVRDLRTMHDLPDTTERVVSIAWAADNRS
                     LFYTVEDEVQKRSYRLYKHAVGSDAKLDPIVYEEKDERFDIYVSRTRSKQYLLLNAAS
                     HISSDVRFLAADQPDGQWKLIAPHVDNVEYYVDHRGDTFYIRTNDTSQTFRLVTAPVA
                     SPGKENWKELVASRPDVPLEDFDVFQNFYALTERVAGLPVFRLVRFSDGSSASIKFPE
                     PTYFATPANNAEFNTATFRYNYQSLITPPSVFDYNVTSKHSTLLKQNEVPGGFDRNNY
                     VSERVFATAGDGTKIPISLFYRKDLKNKAQAPLYLYGYGSYGFSLPVTFSAARLPLVD
                     RGLIVALAHIRGGGELGKPWHDAGKMMNKKNTFTDFIASAEYLTSQGYGAKDKIGIEG
                     GSAGGLLMGAVTNLRPDLFRVVLCEVPFVDVMNTMLDASLPLTVPEYEEWGNPNTEPA
                     FDYMLSYSPYDNLARKDYPTTLVRTSFDDSQVMYWEPAKYVAKLRTLKTDQNPLLFYT
                     NMHGGHGGSSGRYDRIHEVAFNWAFLLTQLGVPEPGDAPKP"
     gene            2343..2852
                     /locus_tag="VFC15_01575"
     CDS             2343..2852
                     /locus_tag="VFC15_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08878.1"
                     /translation="MAVSVVRTFLAITLFCSALGAVAQDSPFGANGPAPTGKQQAAPP
                     PQPTHVPDMVCFGNSPRWSFQFGDTAARTLGISEGDKYWIGKFMYVDGQWSWHGSAAT
                     GSGGGLTASITKGKCIDKTQADTKEFPYQGQVYLPEGDIVNGCCRKLKPGEAPVGPHG
                     YIPSTPPPQ"
     gene            3113..5317
                     /locus_tag="VFC15_01580"
     CDS             3113..5317
                     /locus_tag="VFC15_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZM08879.1"
                     /translation="MNNRFCKQFLSSVAFPLLLSLGTAFAQKPADAPPQNFPWMDRTL
                     SPDERANMVLSRMTLDEKIALLHGNGMAHAAQWQMPLTYLSNGGAGYVEGVKRLGIPP
                     IFMSDAAYGVRDSAENGRYSTALTSNLGSAASWDPQAACRYGALIGRELRAQGYNMTL
                     GGGTNITREPRNGRTFEYMGEDPILAGTLVGNRIRCEQEQHVIGDIKHYAVNDQESGR
                     NEVNAIIGQRALHETDLLAFEIGIGIGQPAAVMCSYNAINGDFACENKYLLTDVLKGM
                     WKFPGFVVSDWGGTHSTVKASAAGLDNEEPMADFFGPKLKQAVESGQVPMSQIDDHAR
                     RILRSEFASGIVDYPTKKSVVDVEDGLETARKIEEQSIVLLKNDKSVLPLDQSKLRSI
                     AIIGFNADIGMISGGGSAQVDPQGSSAPAWLSHVWFPTSPLKAVRAKSRSAEVKFNPG
                     TDPATAAALAKSSDIAIVFAHQWMSEGMDLPSLSLPDIQDALIAAVAAANPRTIVVLE
                     TGTAVTMPWIDRVSAVLEAWYGGSKGADAVANVLFGDVNPSAKLPMTFPLSVNDLPHT
                     TVTQPPVRDRGPESGAVLASPTFEVHYDEGLKVGYKWYDAEKKPVLFAFGHGLSYTTY
                     SYSDIKVTHGKDTTVTFTVKNTGSRAGAEVAQVYAALPTDAGEPPKRLVGWSKVQLNP
                     GESKQVTVPVPPLYLSIYDEAANSWKLLPGSYTFMAGGSSKSLPLTGKVDLK"
     gene            5632..7830
                     /locus_tag="VFC15_01585"
     CDS             5632..7830
                     /locus_tag="VFC15_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZM08880.1"
                     /translation="MSVVRTASNLNQIFRFGEFEFSVRSGELRRNGEILRLQYQPQRV
                     LLVMLEYSGDVVTRDEIRERAWPDDSIRDFDNSLRVAVTKLRQAFGDDPDNPRYIETV
                     PRRGYRWLYPVTVHEAQSIVVGPPALAAQVNVDGQSHPLNGESAPLTSVSLKPSRRTV
                     LLRRILLTIALWVVALAATWWLRPQPVRAEPKVLPLTTYPGLEYMPSLSPDGKRVAFA
                     WTGANPTDPYSVYVRPIGDDRAHRLVETPAGAADGDPVWSPDGKSIYFFRRGGGESSG
                     IYAASVGGGPARQLIATSLAGRRLRRVRFDVSPTEHTLVYPDAIPGKETAALFLLDLA
                     TLRSRQITNPPPNSEGDGDPAFSHDGKTLAFQRSILDLEQIYILPSSGGDARLLTSNF
                     ITDFIDGLAWTSDDREIIFGGSQLRRMSASKGEPSIANISDVPGPATFPAVRGDLLAY
                     VKSAANVNIWKLDLHDLTHAAGEPSKLISSTHQQAAPSFSPDGTRIAFQSDRSGSWEI
                     WVCDRDGSNAVQLSHFGGALTGTPRWSPDGKQIVFDSRANGVSQVYVVSADGGTPRLL
                     TNDAAGGELPSFSHDGKWVYYSSNHNGVTGVWKLPVAGGAPQSVTSGSGIYAAESADG
                     KYLYYCRSAIDFTIWRIPVTGGLEEQVPGLPKPFDTSHWAVVASGIYVVNGNGDLLFF
                     QFGRNTVTTVFHEQRFLTDWSMAISPDGREIAWAQVDDTAADLMLVENFR"
     gene            7956..9371
                     /locus_tag="VFC15_01590"
     CDS             7956..9371
                     /locus_tag="VFC15_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZM08881.1"
                     /translation="MAAAKDHKTSRSIREKLFALLLRLVPTERQRLLVLTILSGGLCG
                     LAAVAFHVGIDKMSGLLIDRAASGPHHSWIYLTILTPTLGGLVAGLGLRYWVPGAVGS
                     GIPQVKLAYAREAGRVPFRDAVGKFVLAVIQIGSGASLGREGPTVQICAGITSLLGKF
                     GKLSRQNRRRMASVGVAAGIAAAFNAPIAAVTFTLEEVIGDLDQTMLSGVIVAAALAA
                     VVERSILGQHPVLEIMRTYTLGSASSLLWYAVLGVLSGIISIAFTDSLLGLRAKFKQL
                     TAVPKWVHPAIGGAVTGTLAVIGLAWLHTGGVAGGGYATLGRALLGSLPLKVMVGLCL
                     LKLAATVFSYSSGGVGGIFAPALFIGAMLGGTVGFIDVAVFHHPAEEIGAFAVVGMGA
                     VFAGIIRAPMTSVLIIFEMTGGYGLVLPLMVANMTSYALARNWRHTPIYEALLEQDGI
                     VLPHGKPVVDPGPDLAGEALP"
     gene            complement(9510..10049)
                     /locus_tag="VFC15_01595"
     CDS             complement(9510..10049)
                     /locus_tag="VFC15_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZM08882.1"
                     /translation="MKTRIFAFVMLAALVATTASAQKVNVDWDRGTDFSKFHTYAWQP
                     STHPAKGLWDQRIIDAVNQQLQAKGFQLAQSNPDLWVVYSNSIKKDQQVVGTGYNMGP
                     GWGWGYGWGGPTTTTYQTYTTREGTLIVDIADANGHTLLWRGSATNSISDNSDKNIKN
                     LNNAVNKLFKNFPPKPGSK"
     gene            10311..>10988
                     /locus_tag="VFC15_01600"
     CDS             10311..>10988
                     /locus_tag="VFC15_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HZM08883.1"
                     /translation="MSLALESRHCGSVYVVRCAGRLVAGQESALLEEALKRGLREFNR
                     VVLNVTEVNYVDSSGMGLMVRFLWHARTRGGDLRLSGPTPFVTKLLEVTKLSAVLRIY
                     ADEESAIVSFLKEGAYTPADSANTGPRVLFLDPSPDTCAFVRALLSRHGYRVLSTGLM
                     GDAKILLNASTVDVIVLGPDSGSLSSSATLDVLQRLAPAAKTVVLEREFKSRAADEAG
                     IALLDRIQ"
BASE COUNT         2206 a         3298 c         3218 g         2266 t
ORIGIN      
        1 ccactttcgc tggcgcgtcg agagcttgtc cgggggaaac tcacgtataa tcagcgctca
       61 ttggttggct ggtccaaccc ccagtggagt gctcatgcta cgagttcttc tggtagttct
      121 gatattggtt cctatgttcg caaactcaca aaattccacc accgccgcac aacctaactc
      181 cccatctcaa ccccaaggca ctgcagtcaa gcctcccgtc gccaagattg ttcacaccga
      241 aaggacggtg aatggcaagc agttggtcga cgattacgcg tggctgcgcg agcgctccaa
      301 tcctgaagtg aaagcgctgc tggaagccga gaacgcctac gcggaattcg tcatgaagcc
      361 gaccgaagcg cggcagaaaa aactctacga cgaaattgtc agccatatta aggagagtga
      421 cgacaccgtc ccgttcctca aggacggcta cttctactac acgcggaccg agaagggtaa
      481 gcagtactac cagctgtgcc gcaagaaagg ctcgctcgat gcgccggaac agatcatcct
      541 cgacctgaac cagatgggcg agggccagaa gttcatggca gtgggccagt ggtcggtcag
      601 cgacgatggc aatctgctgg cttacaccac cgacaacgtg ggattccggc aataccacct
      661 gcatgtgcgc gacctgcgca ccatgcacga cctgcccgac accaccgagc gagtggtttc
      721 gatcgcgtgg gcggcagaca atcgctcgtt gttctacacc gttgaggacg aagtccagaa
      781 gcgcagctat cgactttata agcacgctgt gggaagcgat gccaagctgg atcccattgt
      841 gtacgaggaa aaagacgagc gcttcgatat ctacgtgagc cgcacccgga gcaagcagta
      901 tctgctactg aacgcagcca gtcacatctc gagcgatgtg cgctttctcg cggccgatca
      961 acccgacgga caatggaagc tgatagctcc ccacgtcgac aacgtcgagt actatgtcga
     1021 ccatcgcggc gacacgttct acatccgcac caatgacacg tcacagacgt tccgcctggt
     1081 gacggcgccg gtcgccagcc cgggcaaaga gaactggaag gaactagtgg ccagccggcc
     1141 ggatgttccg ctggaggact tcgacgtgtt ccagaatttc tacgcgctga cggaacgtgt
     1201 tgcgggattg ccggtgttcc ggttggtccg gttcagcgat ggatcgtcgg cgtcgatcaa
     1261 gttccccgaa cctacttatt tcgccacgcc ggccaacaat gccgagttca acacggcaac
     1321 tttccgctac aactaccagt cgctgattac gcctccctcg gtgttcgatt acaacgtcac
     1381 ctcgaagcat tcgacgctgt tgaagcaaaa cgaagtgccg gggggattcg accgcaacaa
     1441 ttatgtctcc gagcgcgtct tcgcgacggc aggtgacggc accaaaatcc cgatctccct
     1501 tttctatcgc aaggacctga agaacaaagc gcaggctccc ctttacctgt atggctacgg
     1561 ttcctatgga ttctcgctgc cggttacgtt cagcgcggcg cgcctgccgc tggtcgatcg
     1621 tggcctcatt gtggcgctgg cgcacattcg cggcgggggc gagttgggca agccgtggca
     1681 cgatgccggc aagatgatga acaaaaagaa taccttcacc gatttcatcg ccagcgccga
     1741 atacctgact agccaaggct atggcgcgaa ggacaaaatc ggcatcgaag gcggcagcgc
     1801 gggcggtctg ctaatgggcg ccgtgaccaa cttgcgtccc gacctgttcc gcgtggtgtt
     1861 gtgcgaagtg ccgtttgttg acgtgatgaa caccatgctg gatgcttccc tgcccctgac
     1921 ggttccggag tacgaggaat ggggcaatcc caacacggag cccgcgtttg actacatgct
     1981 gtcgtattcg ccctacgaca acctggcccg caaagactac cccacgacgc tggtgcgcac
     2041 ctcgttcgac gacagccagg taatgtattg ggagccggca aagtatgttg caaagttgcg
     2101 caccttgaag actgaccaga acccgctcct cttctatacc aacatgcacg gaggacatgg
     2161 aggatcgagc ggacgttacg atcgcatcca cgaggtggct ttcaactggg cattcctgtt
     2221 gacgcaattg ggcgtgccgg agccgggtga cgcgcccaag ccgtagcggc gaatcaatcg
     2281 ccgcagggtt actgccgaaa ggcaagacaa cggctacaat cggatattcc cggaggaccg
     2341 taatggcagt tagtgttgtg cgcacttttc tcgcgatcac tttgttttgt tccgcgctgg
     2401 gcgcggttgc ccaggacagc ccattcggtg ccaacggtcc cgctcccacc ggcaagcaac
     2461 aggccgcgcc accgcctcaa cccacccacg ttcccgacat ggtctgcttc ggcaacagtc
     2521 cgcgttggag ctttcagttc ggcgatacgg cggcgcgcac cctcggcatc agcgagggag
     2581 acaagtactg gatcggcaag tttatgtatg tcgatgggca gtggtcgtgg catggcagcg
     2641 cagcgaccgg cagtggcggc ggtctgaccg cctctatcac aaagggcaag tgcatcgaca
     2701 agactcaggc cgacaccaag gagttcccct accagggcca ggtctactta ccggaaggcg
     2761 acatcgtgaa tggctgttgc cgcaagctca agccgggcga agcccccgtc gggccgcacg
     2821 gatacatccc gtccaccccg ccgccgcagt agtccagtag tccggtgcgg cacaagccct
     2881 gggttgtttg tcattcctcg gaagcttcag ccgcccgggg atctgctttc ctgacatatt
     2941 cactaccctc tcgagccggc gggcttgccg ctcgacccgc cgatctcagt cgagaatccg
     3001 ccctcacggc agaaaaatct cagccacacc ccatgtttgt cttagctcta gacgccacct
     3061 gctggtatgg ttgtacgtga ataatttatg aaccgaggta tctaaatctc caatgaacaa
     3121 tcggttttgt aagcaatttc tttcctccgt tgcctttcca cttcttctct cgctcggcac
     3181 tgccttcgcg cagaaacccg cagacgctcc gccacaaaac ttcccctgga tggacagaac
     3241 gctctctccc gacgagcgcg ccaacatggt gctgagccgc atgacgctcg acgaaaaaat
     3301 cgcgctcctg cacggcaacg gcatggcaca tgccgcccaa tggcagatgc ctttgaccta
     3361 cctctcgaat ggcggcgcag gctacgtcga gggagtgaag cggttgggca ttccgccaat
     3421 cttcatgtcc gacgccgcct acggtgttcg cgatagcgcc gagaatggcc gctactcgac
     3481 cgcgctcacc tccaatctgg gttcggccgc gagttgggac ccgcaagccg cttgccgata
     3541 cggcgccttg atcggacgcg aattgcgcgc gcagggttac aacatgacgc tcggcggcgg
     3601 caccaacatc acccgcgagc cgcgcaacgg ccgcaccttc gaatatatgg gcgaagatcc
     3661 catactggcc gggaccctgg tcggcaaccg tattcgttgc gagcaggaac agcatgtaat
     3721 tggcgatatc aagcattacg cggtgaacga tcaggagagc ggccgtaacg aagtcaatgc
     3781 gatcatcggc cagcgggctc tgcatgaaac cgatcttctg gcatttgaga ttggcatcgg
     3841 cattggccaa cctgccgcgg tgatgtgctc ctataacgcg atcaatggcg acttcgcctg
     3901 cgaaaataag tacttgctca ctgatgtgct gaaagggatg tggaagttcc ctggcttcgt
     3961 ggtgtccgac tggggcggga cccatagcac cgtaaaggcc tctgcagcgg gactcgacaa
     4021 cgaagagccc atggccgact tctttggacc aaagctcaag caagccgtgg aatcggggca
     4081 ggtcccgatg tcgcagatcg acgatcacgc gcgacgtatt ttgcgctccg agttcgccag
     4141 cgggatcgtc gactatccga ccaagaagag tgtcgttgac gtcgaagacg ggctcgaaac
     4201 tgcccgcaag atcgaagagc agagcatcgt cctcctgaag aacgacaaat ccgtactgcc
     4261 gctcgaccag tcaaagctgc gcagcatcgc catcatcgga ttcaacgcag acatcggaat
     4321 gatctccggt ggaggttccg cacaagtcga tccccaaggc agcagcgcac ccgcatggct
     4381 ctcgcacgtg tggttcccga catcaccgtt gaaagcggtg agagcgaagt cccgctccgc
     4441 cgaagtgaaa ttcaatcccg gcacagaccc cgcgaccgcc gcagctttag cgaagtcatc
     4501 cgacattgcc atcgtttttg cgcatcagtg gatgagcgag ggcatggacc ttcccagctt
     4561 gtcgctgccc gatatccagg atgccctgat cgccgccgtg gctgccgcaa atcctcggac
     4621 gattgtagta ctcgaaaccg gcaccgccgt gaccatgcct tggatcgaca gagtgagcgc
     4681 agtcctcgag gcttggtatg gcggcagtaa gggcgcggac gcagtcgcca acgtgctttt
     4741 cggcgacgtc aaccccagcg ccaagctgcc catgaccttc ccactcagtg tcaatgatct
     4801 cccgcacaca accgttacgc aacctcccgt tcgcgaccgc ggccctgaat ccggagcggt
     4861 actcgccagt ccgacgttcg aagtgcatta cgacgaggga ctgaaggttg gttacaagtg
     4921 gtatgacgcg gagaaaaagc ccgtgctgtt cgccttcggc catggactct cgtataccac
     4981 ctacagctac tctgacatca aagtgactca tggaaaggac acgaccgtta ctttcactgt
     5041 aaagaacacg ggcagccggg caggcgcgga agttgcgcag gtttacgcag cccttcccac
     5101 cgatgcgggc gagccgccga agcgactggt gggatggagc aaggtgcagt taaatcctgg
     5161 cgaaagcaag caggtcactg tgcccgtgcc tccactctac ctctcgatct acgacgaggc
     5221 tgccaatagc tggaagctgc taccagggag ctacacgttc atggccggtg gctcgtcgaa
     5281 gagcctgcca ctgacaggga aggtcgacct caaatagtct tgtagtctca attcgacagc
     5341 atatagccca gttcacggtc acgaactggg cttttctaca ttaacgagag attatctacc
     5401 ttaattttgt ccactgaagt taattatgga gagccgcaac caatatttta gtttcgccgt
     5461 atagtggcta tacgatggat caattccgcc aaccgtcggc acgaaaccgg tcatatctcc
     5521 ctgactcgga gatttgccgc ctaagctgtt gactcttctg tgcattcagc gcttgccgca
     5581 cgggtgggca gtgggtatga ttatttgatt ccccggttta gtaggtgcgc gatgtcagtg
     5641 gtgcggaccg cttctaacct taaccagatc tttcggttcg gtgagtttga gttctccgtc
     5701 cgttccggcg agttacgcag gaacggtgaa atcctgcggc tgcagtacca acctcaacgg
     5761 gtgctactgg tgatgttgga gtactcgggc gacgtagtga cccgtgacga aatccgcgaa
     5821 cgggcctggc ctgacgattc catccgggac ttcgacaata gtctgagagt agcggtaacc
     5881 aagcttcgcc aggcatttgg cgatgaccct gacaatccgc ggtacatcga aaccgtgccc
     5941 cgacggggct accgctggct ctacccggtc acggtacacg aggctcagtc gatcgtcgtc
     6001 gggccccccg ccctcgcagc acaggtcaac gtcgacggcc agtctcaccc actgaatggt
     6061 gaatcggcgc cgctaacttc agtttccttg aagccttcgc gccgcactgt cctgttgcgg
     6121 cggatccttc tgaccattgc cctttgggtc gtggctttgg cggcaacttg gtggttacgt
     6181 ccgcagccgg tgcgcgcaga acctaaggtc ttgccgctca ccacctaccc gggcttggaa
     6241 tatatgccgt cgttgtcgcc tgacggaaag cgagtcgcgt tcgcctggac cggtgcgaac
     6301 cccaccgatc cctacagtgt ttacgtcaga ccgattggcg acgacagggc acatcgcctg
     6361 gtggagacac ccgccggggc agccgacgga gatcccgtat ggtcacctga cggaaagtcg
     6421 atttactttt ttcgccgcgg cggcggcgaa agctcgggaa tctatgccgc gtcagtcgga
     6481 ggcgggcccg ctcgccaatt gattgcgacc tctcttgccg gccgccggct ccggcgggtc
     6541 cgttttgacg tatcgccgac ggaacataca ctggtttatc ctgatgcaat tcccgggaaa
     6601 gagacggcgg cattgtttct gcttgacctg gccacgctaa ggtcccgcca gatcaccaat
     6661 cccccgccga actcggaagg agacggagac ccggcgttct ctcacgacgg caagactctg
     6721 gcgttccagc gcagcatcct cgacctggag cagatctata tcctgccatc gagcggcggc
     6781 gacgcacggc tgctcacgtc caacttcatt accgacttca tcgacggctt ggcgtggacc
     6841 tccgacgacc gcgaaataat tttcggagga agccagctgc ggcgtatgtc cgctagcaaa
     6901 ggggaaccgt cgattgccaa catctccgac gtgcccgggc cggcaacgtt tccggcggtg
     6961 cgcggcgact tgcttgcgta cgtaaagtcc gcagccaatg tcaacatctg gaagctggat
     7021 ctgcacgact tgacccacgc agctggagaa ccgtccaagc tgatttcgag cacccaccaa
     7081 caggctgccc cgtccttctc gcctgacggc acacgcatcg ccttccagtc cgatcgtagc
     7141 gggagctggg agatatgggt ttgtgatcgt gacggctcga acgcagtcca attgagccat
     7201 ttcggcggag cgctcacggg tacgccgcgt tggtcaccag acggaaaaca aattgtgttc
     7261 gactcacgcg ccaacggagt gtcgcaggtg tatgtggttt ccgcggatgg cggtacgccg
     7321 cgcctactca ccaacgacgc agccggtggc gagctgcctt ccttctcgca cgacggaaaa
     7381 tgggtctatt actcctcaaa ccacaacggt gtaaccggcg tctggaagct gccggtggcg
     7441 gggggcgctc cgcaatccgt caccagcggg agcggtattt atgccgcgga gtcggccgac
     7501 ggcaagtacc tctactattg ccgcagtgcg attgacttta caatctggcg cattccggtt
     7561 acgggcggcc tcgaagagca ggtgcccggt ctgcccaagc ccttcgacac ttcgcattgg
     7621 gcggtggttg cgtccggaat ttacgtcgtc aacggaaacg gggacctgct gttctttcaa
     7681 ttcggcagga ataccgtgac gacggttttc cacgagcaaa ggttcctcac cgactggtct
     7741 atggcaatct cgcccgacgg tcgcgagatc gcatgggcgc aggtcgacga tactgccgcc
     7801 gacttaatgt tggtcgagaa ctttcgctag tctctgcttc gcccgaccca tcgtgaaact
     7861 ttgctggaac caaacgcttc gtcacggcgt tgtgagcccc gacgcgagaa ttccttttat
     7921 tattagagat agcaagatcg atcacaaagg tgaggatggc ggcggcgaaa gatcacaaga
     7981 catccaggtc aattcgggag aaactgttcg cgctgctgct gcgactggtt cctactgagc
     8041 gccagcgtct gctggtgctg acgattctca gcggcggcct gtgcggtctg gccgcagtgg
     8101 ccttccacgt cggcatcgac aagatgtcgg gcctgcttat tgaccgcgcc gcatccggcc
     8161 cacaccattc ctggatttac ctgactatcc tcactcccac gctgggtgga ctggtggccg
     8221 ggctcgggtt gcgttactgg gtccccggag ccgtaggcag cggcattccc caggtgaaac
     8281 tcgcgtatgc ccgcgaggcg ggccgggtgc cgtttcgcga tgcagtgggg aaattcgtgc
     8341 tcgcagtaat ccagatcggc agtggagctt cgctgggccg cgaagggcca acggtgcaga
     8401 tctgcgctgg catcaccagc cttctcggca agttcggaaa gttgtcgcga cagaaccgca
     8461 ggcgcatggc ttcggtggga gtagcagccg gcatcgccgc agctttcaac gcgccgatcg
     8521 cggcggtgac cttcaccctc gaagaggtca tcggcgacct ggatcagacg atgctttcag
     8581 gtgtgatcgt ggccgctgcg ctcgccgccg tggtggagcg cagcattctg ggccagcatc
     8641 ccgtgctgga aatcatgcgg acgtatacgt tgggttcggc gagttcgctg ctttggtatg
     8701 ccgtgctggg agtactatcc gggatcattt cgatcgcctt caccgattcg ctcctcggac
     8761 tgcgcgcgaa attcaagcaa ctcaccgcag tgcccaaatg ggtacaccca gccatcggcg
     8821 gcgcggtgac cgggactctc gcggtcattg gactggcgtg gcttcatacc ggaggggtgg
     8881 ccggcggagg atacgccacg ctggggcgcg cgctgctggg ttcgcttcct ctcaaggtga
     8941 tggtgggact atgcctgctg aaactggccg cgacagtctt ttcgtacagc agcggaggag
     9001 tgggcggaat cttcgctccg gcgctgttca tcggagccat gctgggaggc acggtcggct
     9061 tcatcgacgt tgcggtcttc caccatccgg cggaggaaat cggggcgttc gccgtggtgg
     9121 gaatgggagc ggtgtttgcc ggcatcattc gcgctcccat gacctcggta ctgatcattt
     9181 ttgaaatgac gggcggctac ggcctggtgt tgccgctcat ggttgccaac atgacttctt
     9241 atgcgttggc ccgcaactgg cggcacactc caatctacga agctctgctg gagcaggacg
     9301 gaattgtctt gccacacgga aaaccggtcg tcgatcctgg gccggatctg gccggcgaag
     9361 cgcttcccta gcgaatccga cattgcacgc gtccccagga gcggttcttg cgcgtcgcga
     9421 gaaaagttgc gcgcaagcaa aatccccaca cctgcgtgtg aagggatggg gattgccgct
     9481 gcgcgaggca gcagatagat gtccgattcc tatttgctgc ccggcttggg aggaaagttc
     9541 ttgaatagct tgttcaccgc gttgttcagg ttcttgatgt tcttgtcgga gttatcggaa
     9601 atgctgttgg tcgccgagcc ccgccacagc aacgtgtgtc cgttggcgtc ggcgatgtca
     9661 acgatcagcg tgccttcccg agtggtgtag gtttgatagg tcgtcgtggt agggccgccc
     9721 cagccgtatc cccatcccca acccggtccc atgttgtagc ccgtaccgac tacctgctgg
     9781 tctttcttga tgctgttgct ataaaccacc cacaagtcag ggttcgactg cgccagctga
     9841 aatcccttgg cttggagctg ctgattgacc gcgtcaatga tgcgctggtc ccacaatccc
     9901 ttggccggat gcgtgctcgg ctgccaggcg taggtgtgga acttggagaa gtcggtgcca
     9961 cggtcccaat cgacgttgac tttctgcgca gacgctgtgg tggccaccag cgccgcgagc
    10021 ataacaaatg cgaatatgcg tgtcttcatt ggcaatggct ccctatgcga accggtacaa
    10081 ctgtttagat ggcgcggcac ggaacctgcc gtcgcttgct gcaatcgtac catcggctcg
    10141 cagtccgtgt gcgacggcag gcgaaggacg ggcagccttc aacctccacc actaccgggc
    10201 catatcggct ggccacctta tggcgggcga acgattggca ctcgtcgagg ttgcctgtgt
    10261 gctgtaagat taccgcgagt tgagcgcgcc acggaaagcg ggtgctgggc atgtccctgg
    10321 ctctggaatc tcgtcattgc ggatcggtat acgtcgttcg ctgtgcaggc cgtttggtcg
    10381 ccgggcaaga atctgcgctc ctcgaagaag cgctcaaacg ggggttgcgt gaattcaacc
    10441 gggtggtgct gaacgtcact gaggtgaatt acgtggacag ttccggcatg gggctgatgg
    10501 tgcgcttttt gtggcatgcg cgcactcgcg gcggtgacct gcgtttgtct ggtcccacac
    10561 ccttcgtcac caagctgctg gaggtcacca agctctctgc ggtgctccga atctatgctg
    10621 acgaagaatc cgcgatcgta tcgtttctga aagagggcgc ctacaccccc gctgactccg
    10681 ccaacaccgg accgcgcgtg ctgtttctcg acccctcgcc tgacacctgt gccttcgtgc
    10741 gcgccttgct gtcgcgacat ggctatcgcg tactctccac gggactgatg ggcgacgcca
    10801 agattctgct gaacgcttca accgttgacg tcatcgtgct gggtcccgac agcggctcgt
    10861 tgtcatcgtc ggccacgtta gatgttctgc agcgcctcgc tcccgcggcg aagaccgtcg
    10921 tgctggagcg ggaattcaag agccgtgctg ctgacgaagc aggaattgcc cttctggacc
    10981 gaatccag
//
LOCUS       DASRKK010000078         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_786360, whole genome shotgun sequence.
ACCESSION   DASRKK010000078 DASRKK010000000
VERSION     DASRKK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786360"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFC15_01605"
     CDS             <1..827
                     /locus_tag="VFC15_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08884.1"
                     /translation="QQIFEDFVAFVDFTDSKGVYLTRFLNPNVGPTAALPGQACPGQA
                     ATPSSADTVTYCGDPPFPLLGSITNTISNAKSLYRGLTVGLRKRMSHHFLAEAEYTYS
                     VDRDDDSNERDPFTFRYANLYNLKSEYSISDRDERHKFNAYAVGDLPFGINGNLRIQQ
                     HSAEPETDNSNGTGTGAPCSLNNSATRFVDGIDCGRNHLRKDNAFFVFDFGVARPFRF
                     HDGKLSLIPRVEVFNTFNNTNNLNPLSSPALFDFSGFLRVGVGDPRQAQLSLRFAF"
     gene            complement(841..1482)
                     /locus_tag="VFC15_01610"
     CDS             complement(841..1482)
                     /locus_tag="VFC15_01610"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM08885.1"
                     /translation="MASVEEVGSAAEKKSLHAVERDTEANRERREEFAARIQSISPER
                     LIFLDESGVTTSMTRLYARGAGGKRIQEATPGGHWKIMTILGAMSLSGMVATMTIEEP
                     TDTDIFLAYVEHLLYPVLKPGDVVVMDNLSSHKAPAVREWIEKAGAEVLYLPPYSPDL
                     NPIEKAWAKLKQLLRAAKARTKETLDQAITEALPCITPANAKAWFRIALDGLR"
BASE COUNT          442 a          677 c          541 g          491 t
ORIGIN      
        1 atcagcaaat cttcgaagac ttcgtagcct tcgttgactt caccgactca aagggtgttt
       61 acctgacgcg tttcttgaac cccaacgtag gaccaaccgc tgctctgccc ggacaggcct
      121 gccctggaca agcagcgaca ccctcaagcg ccgataccgt cacttactgc ggtgatccgc
      181 cgtttccact gctgggctcg atcactaaca ccatcagcaa cgctaagtcg ctctaccgcg
      241 gacttacagt cggtcttcgc aagcgcatgt cgcaccactt tctggcagag gcagaataca
      301 cctactcggt tgatcgtgac gacgactcaa acgaacgcga ccccttcact ttccgctacg
      361 ccaatctgta caacctcaag tcggagtatt ccatttcgga tcgtgatgag cggcacaaat
      421 tcaatgccta tgcggtgggc gacctcccct ttggaatcaa cggaaacctt cgcatccagc
      481 agcattccgc cgagcctgaa acagacaaca gtaacgggac cggaaccggc gctccgtgca
      541 gtctaaacaa ctccgcgacc cggtttgttg atggcatcga ctgcgggcgc aaccacctcc
      601 ggaaagacaa tgctttcttc gtattcgact tcggcgtggc tcgccccttc cgtttccacg
      661 atggcaagct ctctctaatc ccgcgcgtcg aggtctttaa cacctttaac aacaccaaca
      721 atctcaatcc gctcagctca cctgcgctgt ttgacttcag cggcttcctc cgcgtcggag
      781 taggtgatcc gcggcaggct caactctctc tccgcttcgc gttctagagc attttctgtt
      841 ttaccgtaga ccgtcgagag ctattctgaa ccaagctttt gcattggcag gagtgatgca
      901 tggcagagcc tcggtgatgg cctgatcgag tgtttctttg gttcgtgcct tggcggcgcg
      961 aaggagttgt ttaagttttg cccaagcctt ctcgatcggg tttagatcgg gagaataggg
     1021 cggcagatag agcacctccg caccggcttt ttcgatccat tcgcggaccg ctggcgcctt
     1081 atgcgagctc agattgtcca tcaccaccac gtcgccgggc ttcagcaccg ggtacagcag
     1141 atgttcgacg taggccagga aaatatcggt gtcggtgggt tcttcgatgg tcatcgtcgc
     1201 gaccatgccg ctcagactca tggcgccgag aatcgtcatg attttccagt ggccgccagg
     1261 cgtcgcctcc tggatgcgtt tgcctcccgc gcctcgcgcg tagagccgtg tcatcgaggt
     1321 agtgacacca ctctcgtcca gaaaaatcaa ccgttccggc gagatcgact ggatgcgcgc
     1381 cgcaaactct tcgcgtcgct cgcggttggc ttcggtgtcc cgctcaaccg cgtggagtga
     1441 ctttttttca gccgcagacc caacttcctc aacagatgcc agatccgtcc cgagctcagc
     1501 gaaataccag catcgctgtg caacttggcc tgcagttgag ccagcgtcag atcaggcttc
     1561 gaagccaccc aatccatcac ctgacgctgc gcctcgacac cggcccgcag cttacgtcca
     1621 ggttggtgcg gcacccgctc ggcctgcccc gtccgcttcc gttgtgcgga aatcttcttc
     1681 gcccagccca cgctcacaag gaagcgatcc gcaagttcct ccagcgtctc ctcgccgcgg
     1741 tcgtaggaca acagaaactt acggcgcaga tcatccccat atggcttcgc cattccattc
     1801 actcgccgca gagtctcgcc aattgaagtc tacactattt cagaaaatgc tctaattcga
     1861 ttggcgactg atagactgcc agccgcatag ggcagacgtc gtttttgcat gttgtctaca
     1921 tgacaacacc gctcattgag cgcgtctcag agatgatcgc aacgtttagt cctgacgcaa
     1981 gacgtttgcg gaaggttggc cagcagtgcc ggcgcgaaat cccgccgata caagtgcgta
     2041 tccgttcgtg ctccccaaga cctctctttg aaccctcagt taacgcattc agggcgattc
     2101 gttcatcgaa ctcaggttag aaagctggct gcgggttagt cccgatagac g
//
LOCUS       DASRKK010000079         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_199934, whole genome shotgun sequence.
ACCESSION   DASRKK010000079 DASRKK010000000
VERSION     DASRKK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199934"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFC15_01615"
     CDS             <1..763
                     /locus_tag="VFC15_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08886.1"
                     /translation="FVRHDSTEENLRLLGRYLTTHGRPLAVYTDKASLFQTAAKGIHH
                     RDAPAEQPTQIGRPLRELSIEWIAAHSPQAKGRIERFFGTAQDRLVKGLRKVGASTLK
                     QANTYLSGTYLPLWERRFSHAPRQAGDANRPLDAGLDLDSVLSRVELRVVAQDYTVRW
                     QGETYQIPRPQIGSGMRGSQVAVEKRLDGSVWMRWKQRRRLGPCPLAVTANYRVARVA
                     TPAAVAKPGHPNSLVQSFSRTRLSVPRTPQNQNAP"
     gene            1299..2084
                     /locus_tag="VFC15_01620"
     CDS             1299..2084
                     /locus_tag="VFC15_01620"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM08887.1"
                     /translation="MSSSSTNRAPEHPLSADTRFDRRLGEILDYATEVFAEKGYEGAS
                     MRDLSRLSGISLAGLYYYFESKEKLLYFIQQHTFNTIIERLRERLQESHDPETRIRIF
                     VHNHISYSVAKQKAMKVLAHEDDVLKDGFGTELAAIKREYYRICVGLVEDLVKAEGLK
                     CVGRHAGPTGGISTRTAVMGLFGMMNWLHTWYRPHVDPDAETIAGEMSNIFLRGVRAE
                     KAADWKPRRAPNLSRKAETNGRPRAKGTKRKPAPSLATGRTRS"
     gene            2251..3030
                     /locus_tag="VFC15_01625"
     CDS             2251..3030
                     /locus_tag="VFC15_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZM08888.1"
                     /translation="MTTATQVGFNRITVELQPPAARIALNNPLLNVIDIPMMDELRAA
                     LEQIEARAEISTIVFAGSERAFSVGVDIAAHMPDKVRDMLTAFHSVIRSVVGSRKLTI
                     ASVRRHCLGGGAELAMMCDMVYASPDSVWGFPEIKLACYPPVAAVALSAIVGQKLAAE
                     LVLTGRTLNGQEALEAGLVNGLADDPETLVTECVQRVSQLSSAAIALAKKAFYAWDAI
                     HFDKGLARAEQIYFDELMKTTDAQEGITAFVERRRPKWVGK"
     gene            3027..3482
                     /locus_tag="VFC15_01630"
     CDS             3027..3482
                     /locus_tag="VFC15_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08889.1"
                     /translation="MKAARKQLLLDTDVVTFSLAIVGLLGCAGLAIRDWHQVLQLRAS
                     PHRAAWQDYFGFVYALWIFVGVKGDPKVVRAVRLLVGLLLVVMSIPIAVSLMHLSRGT
                     ARALSLWSELTSAFLFTTAALFVLVWFRGKFRDARQRSNLDKDSLKEQL"
     gene            3485..>3688
                     /locus_tag="VFC15_01635"
     CDS             3485..>3688
                     /locus_tag="VFC15_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZM08890.1"
                     /translation="MDYEIAPEELKAQQEAGSDVVLLDVREPWEFEIAKIANSKLIPM
                     GDIPARFNQELDPEKHIVVVCHHG"
BASE COUNT          755 a         1040 c         1132 g          761 t
ORIGIN      
        1 ttttgtgcgg cacgactcga cggaagagaa cttgcggctg ctgggtcgct atctcactac
       61 ccacggacgg ccactggcgg tgtataccga caaggcgagt ctgtttcaga ctgcggccaa
      121 agggatccac caccgcgacg ccccggctga gcagccgacg cagatcgggc ggcccttgcg
      181 ggagttgagc atcgagtgga tcgccgccca ctcgccgcag gccaaggggc ggatcgagcg
      241 cttcttcggc accgcccagg accggctggt gaaaggcttg cgcaaggtag gggccagcac
      301 gctgaagcag gccaacacgt atctcagcgg cacctatctg ccgctatggg agcgtagatt
      361 cagccatgca ccgcggcagg ccggcgatgc caaccggccg ctggatgcgg gcttggatct
      421 ggattcggta ttgagccggg tggagctacg ggtggtggcc caggattaca ccgtgcgctg
      481 gcagggcgag acctatcaga ttccgcggcc gcagatcggg agcggcatgc ggggcagtca
      541 ggtggccgtg gaaaagcgtt tggacggcag tgtgtggatg cgatggaaac agcggcggcg
      601 gctcggaccc tgcccgttgg cggtgacagc gaactatcgc gtcgcccgcg ttgcaacgcc
      661 ggctgcggtt gccaaacctg gccaccccaa cagtttggtc cagtcgttct ctcgcacccg
      721 actgagtgta cccagaactc cccaaaatca aaatgcccca tgaaatcagg ggctgttcag
      781 attcctgctc agaaccgttt attcagatac ccaccgctga agcgctgggc tatcttgttc
      841 cgtcccgcct ggcgggactg gttgattctg gttcgggcat gattgccaac aacgcgcttt
      901 tcaatccttc ccgcgaagcc gagtatgcga gcaagtgtca cgagccctga ttggctgact
      961 gcttgagtac taattgccaa ttgccaattg cagccagttg ctaattgcta attgctgcca
     1021 ctcccaaact gctcgtcctc atcatcctcg atggctgggg ctatgctccg gcgtccaatg
     1081 ccaatgccat ctcgctggcg cgtaagccca actatgatcg tctgctcgcc gaattcccca
     1141 acacgctgat ccaccagaac gaagtggatc gcgttgaggc gcgcccgcct tgggggcggg
     1201 ccggttgccg aattggccgg gtgagaataa aattagaacg aacgttcgaa accgactctt
     1261 ttttccgtag tcgttgcttt ccgactaacg attaacccat gtcctcttcc tccacaaacc
     1321 gggccccgga acatcctttg tcggccgata cccgcttcga tcgccgtctg ggcgagattc
     1381 tcgactacgc caccgaggtg tttgccgaaa aaggctatga gggcgcttct atgcgcgact
     1441 tatcgcgctt gagcggcatc tcgctggcgg gcctttacta ttatttcgaa tccaaagaga
     1501 agctgctgta tttcatccag cagcacacct tcaacaccat tatcgagcga ttgcgcgagc
     1561 gcctgcagga gagtcacgat cccgagaccc gcatccgtat cttcgttcac aatcacatta
     1621 gctattccgt cgccaaacaa aaagcgatga aggtgctggc gcatgaagac gacgttctga
     1681 aggacggctt tggcaccgaa ctggccgcca ttaagcgcga gtactaccgc atctgcgtcg
     1741 gactggtgga ggatttggta aaggctgaag ggttgaaatg cgtcggccgg catgccggcc
     1801 cgacgggcgg gatctccacg cgcacggcgg tcatgggact cttcggaatg atgaactggc
     1861 tgcacacctg gtacaggccc catgtcgatc ccgacgcgga aaccatcgcc ggcgagatga
     1921 gtaatatttt tctgcgggga gtgcgagcgg aaaaagccgc cgactggaag ccgcgtcggg
     1981 cgccgaacct cagccgtaag gcggagacca acggccgccc cagggcaaaa ggcaccaagc
     2041 gcaagcccgc tccctcgctg gcgaccggcc gaactcgctc atgactgcat tgcagcaata
     2101 ccgtgtcagg agttttgccg atgggtagca cgacccaacc tgactgcaag aagttgtcat
     2161 gctgagcgta gtgagcgaag cgaacgaagt cgaagtgcct gtcccgagcg aagccgacgg
     2221 gacctgcggt tgtttggagg aggtgcatcc atgaccacgg ccacacaagt cggttttaac
     2281 cgcatcactg tcgagttgca gccgccggcg gcgcgcatcg cgctgaacaa tccgttgctg
     2341 aacgtcattg acatcccgat gatggatgag ctgcgcgcgg cgctggagca gatcgaggcc
     2401 cgagcggaaa tcagcaccat cgtcttcgcg ggttcagagc gcgctttctc cgtcggcgtc
     2461 gacatcgctg cgcacatgcc cgataaagtg cgtgacatgc tgaccgcatt tcacagcgtg
     2521 attcgctccg tggttggcag cagaaagctg acgatcgcca gcgttcggcg gcattgccta
     2581 ggcggaggcg cggaactagc gatgatgtgc gatatggttt acgcgtcacc ggattcggtt
     2641 tggggatttc ccgagatcaa gctggcgtgc tatccgccgg tcgccgctgt ggccttgtcc
     2701 gccattgtgg gacaaaagct ggcggcggag ctcgtcctta ccggacgaac cctgaatggc
     2761 caggaggcgt tggaggcggg attggtgaac ggcctggccg acgatcccga aaccctagtc
     2821 acggaatgcg tgcagcgggt gtcgcaattg agttcggcgg ccatcgccct cgccaagaag
     2881 gcattctatg catgggacgc gattcacttc gacaaaggtc tggcgcgcgc cgagcagatt
     2941 tatttcgacg agttgatgaa aacaacggac gcgcaggaag gcatcaccgc attcgtcgag
     3001 cgacgacggc cgaagtgggt ggggaagtga aagccgcgcg caagcaattg ctgcttgaca
     3061 cagatgtagt gacgttctcg ttagcgatcg tcggccttct cggctgcgct ggtctggcaa
     3121 ttcgcgattg gcatcaagtt ctgcagcttc gggcatcgcc acatcgtgcc gcttggcagg
     3181 actactttgg gttcgtatac gcgctgtgga ttttcgttgg tgtgaaaggt gacccaaagg
     3241 tcgtcagagc tgtacgttta ctcgtgggat tgctgttagt cgtaatgagc atcccgattg
     3301 ctgtttcgct gatgcatctc tccaggggaa cagctagggc gctatcactc tggtcggaac
     3361 tgacctcggc atttctgttc actacggcag ctttgtttgt ccttgtctgg ttcaggggca
     3421 agtttcgtga tgccaggcag cgtagcaatc ttgataaaga ttcactgaag gaacagcttt
     3481 gagcttggat tacgagatcg caccggagga gctgaaagct cagcaagagg cgggatcaga
     3541 tgtcgtgctg ctcgacgtcc gcgagccttg ggagttcgag atcgcgaaga ttgcgaacag
     3601 caagttgatt ccgatgggag atattccggc gcgctttaac caggaactcg atccggagaa
     3661 gcacattgtg gtggtttgcc atcatggc
//
LOCUS       DASRKK010000080         9497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_786714, whole genome shotgun sequence.
ACCESSION   DASRKK010000080 DASRKK010000000
VERSION     DASRKK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786714"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFC15_01640"
     CDS             <1..1006
                     /locus_tag="VFC15_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZM08891.1"
                     /translation="TGCSSIYGGNLPTTPYTKNKEGRGPAWSNSLFEDNAEFGMGMRI
                     AVDKQKEYAEELVKRMASLIGEELATGLLSADQSTEPGIFTQRDRVKALKEKLAGNKT
                     AAARDLLAVADELVNKSVWIIGGDGWGYDIGFGGLDHVLASDANVNILLLDTEVYSNT
                     GGQMSKSTPLGAVAKFAAGGKRTPKKDIAMMAMNYGRAYVAHIAMGGSDGQTMKAFAE
                     AEAHNGPSLIIAYSHCIAHGYDMVHGIEQQKNAVLSGHWPLFRYNPGLAKEGKNPFQL
                     DSRAPSIPLEKYIYNEARYTMLVSSDPEEAKLLLHQAQDNVNERWKLYQHMAAMSFEK
                     "
     gene            1022..2047
                     /locus_tag="VFC15_01645"
     CDS             1022..2047
                     /locus_tag="VFC15_01645"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HZM08892.1"
                     /translation="MIDLTTNYLGLKLKNPVVVSASPLTEKLENFERLRDAGASAIVM
                     YSLFEEQIEAESENLDAAMELGTNSYAEATTYLPDMPKYQIGPDRYIDLIYKAKKTVD
                     VPIIASLNGNSRGGWTRYAEHMEQAGADALELNIFNIPTDPSVTAEQLEHGYCELVRA
                     VRKAVKIPVSVKLGPYFTSMANFVKQLSAAGADGVVIFNRFYQPDFDLEELEVTPNLV
                     LSSPHELRLRLHWAAILYHQVETYIAITGGVHDSTDVLKSMMAGAHVAMMTSALLKYG
                     IEHIGVVLSGMERWMEEHEYQSVKQMRGSMSLVNIENPTAFLRGNYLKMLGSYKFGEA
                     GSALPTY"
     gene            complement(2257..3384)
                     /locus_tag="VFC15_01650"
     CDS             complement(2257..3384)
                     /locus_tag="VFC15_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZM08893.1"
                     /translation="MKRAQSFFIAAILIFPFWCANSLSAAPPPAAATATAKSVDVSIN
                     GDSLWIDTGMDVNPGDKLHITATGTVDFSDKTGVGPAGAQRGWADTLRALSVSSAGRG
                     ALVGQVGNDRAATPFLVGADATITSPTTGRLYLGVNQDQTSKPTAGKFEVHIDRTAAP
                     AAAANPTNYDFRPLFAIIDQKLPYRVTDQAAPGGNEGDLVNFVLVGSEKQVTNAFKTA
                     GWMLPDKTNQDAVVSALIATLQKQAYVTVPMSTLYLFGRGQDYGFARAEAVKVIGERD
                     HFRIWQTPFKGPNGQTLWAGAGTHDIGIEKDQRKENAITHKIDPAVDGERDFIASTLQ
                     QAGMVQALGYMDRPKQIKETKTATGGEIKTDGRTLVIVLKP"
     gene            complement(3576..4667)
                     /locus_tag="VFC15_01655"
     CDS             complement(3576..4667)
                     /locus_tag="VFC15_01655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZM08894.1"
                     /translation="MLTTAPVTTLAIADRLRALERLIGNTPLLAIEYEVSGRKRTIYA
                     KSEQMNLTGSIKDRMALHIIRRAYAEGRLHPGDTIAEATSGNTGIAFAAVGRALGHPV
                     TIFMPNWMSRERMDLIHSLGANIILVTKQQGGFLGSILMSEEMAMERQDVFLPRQFSN
                     TANVEAHAQTTGPELYLQLKSEGLTADAFVAGVGTGGTVMGVGRYLKQQNPATRVYPM
                     EPSESPTLSTGCKLGHHRIQGISDEFVPAILQLDDLDPVINVSDGDSILMAQKLACEL
                     GIAVGISSGANFLAAVKAQDDLGDDAVVATVFADDNKKYLSTDLLRDEPVKSHYRTPG
                     ITLTVFRTVRRVCNVCGDAEGCDQRVTIV"
     gene            complement(4820..5671)
                     /locus_tag="VFC15_01660"
     CDS             complement(4820..5671)
                     /locus_tag="VFC15_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM08895.1"
                     /translation="MSTEPVIAHDAHIPAPAMIQSSAPSSLQSRLHKHEQFASRLIDE
                     KHDFIVYVPQVFRHDTNRFFPVLYLHDGQNLFDPETSFIKGNYWRVGETADALIDAGE
                     VEPLVIVGIYNTGVKRIDEYTPVEDKRLGGGRADAYGQMLVEELKPFVAHQYRTLAGA
                     ENCGMGGSSLGGLVSLYLGVRYPDIFGNLAIMSPSVWWHNRAILRTIAEIRRNTGQRI
                     WLDMGTSEGKRALRDARALKRLLLQKGWKKGIDLEYREIEHGQHSEYAWAERVGPMLK
                     FLFPRRG"
     gene            5813..6514
                     /locus_tag="VFC15_01665"
     CDS             5813..6514
                     /locus_tag="VFC15_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM08896.1"
                     /translation="MHREFHNWWSKDLQRDMSLLCFGHAGRKVLVFPTSRGRFFEYED
                     NKMIEAVGDKLNNGELQIYCVDSVDDESWYNKSAHPYWRLQRHLQYERYIVNDVFPLM
                     GNKNWTPHTTVTGCSFGAYHAVNFAFRHPDMVNGCVSMGGAFDIRQFLDGWYSEDVYF
                     NNPPDYLANCADGWKYNHMRTVLATGEHDMCWDRNEKFAGILRAKGIRHEIHVWGDRS
                     YHDWPWWRPMARVYL"
     gene            6725..7735
                     /locus_tag="VFC15_01670"
     CDS             6725..7735
                     /locus_tag="VFC15_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08897.1"
                     /translation="MKKIGVIFGMETTFPPALVDKINFMNEPGIQAELATVGEVRMAE
                     PSPYHVIIDRISHDIPFYRAYLKNAALSGAIVINNPFWWSADDKFFNFALGEKLGVAI
                     PQTVLLPHKQHPPGTTDRSMRNLQFPLPWDSIFSYVGFPAFLKPFDGGGWKDVYKVNN
                     PEELFKAYDQSRDLCMTLQHGVKFKEYFRCYVVGQEKVHIMHYDPSAPFHERYVKNPP
                     PVDPRLLERVERDALTLCRALGYDLNTVEFACEDGVPYAIDFMNPAPDADINSVGEDN
                     FHWIVDAVAELAVRKAKQHTGNVSEYRWSAFLGGNPAEVKSEATAKDAKAVKAPKSAT
                     KG"
     gene            7741..8871
                     /locus_tag="VFC15_01675"
     CDS             7741..8871
                     /locus_tag="VFC15_01675"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HZM08898.1"
                     /translation="MKRPSLSIGIEEEYQTIDPETLDLRSHIQSEILPKAILSTNSRA
                     KAEMHQAVIEVGTKVCRDIQEARTDMLELRREMISLANANGLLLGAASTHPFADWKAQ
                     EIYPDERYRQVVEDMQLVARANLVFGLHVHVGIEDRNTAIHIMNSLRYFLPHILALST
                     NSPFWMGMQTGFKSYRCKVFERFPRTNIPDVYANWAEYETFINLLIKTHCIDNGKKVW
                     WDIRPHPFFDTLEVRVCDIPMRLDETLAIAALIQATVAMLWNLHATNKSYRIYSRALI
                     MENKFRASRYGLDGKLIDFGLEEEVPTRELMLEYLNWIDPVLDELGSREEINYIHQML
                     AMGTGADRQLRVFQETGDLRKVVEYIVAETRVGVFDSEPAHR"
     gene            8914..>9497
                     /locus_tag="VFC15_01680"
     CDS             8914..>9497
                     /locus_tag="VFC15_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08899.1"
                     /translation="MTPPLLTDLTTTPFDPELTPGAFNAVHTCLRIQPDEKVTVITDN
                     ACRDIAASIAQELVKVGSPFKAFVLEEIAERPLTGLPRDIADDMETSQVSIFAVNVQR
                     NELKSRMEMTDIVNRRRMRHAHMVNINHEIMLEGMRADFNRVDALSKKVMEIVTQAKQ
                     VHGKNPAGSDLVADLNPNYKWIKTSGIISPDKWG"
BASE COUNT         2023 a         2717 c         2856 g         1901 t
ORIGIN      
        1 taccggttgc tcgtcgatct acggcggcaa cctgccgaca acgccgtata ccaagaacaa
       61 agaaggccgt gggcctgcct ggtcgaactc gttgtttgaa gacaacgccg agtttggcat
      121 gggcatgcgt atcgcggttg acaagcaaaa ggaatacgcc gaagaactgg tgaagcggat
      181 ggcatcgctt atcggcgagg agcttgccac aggcctgctg agcgccgacc agtcaaccga
      241 gcccggcatc ttcactcagc gcgaccgggt aaaggcactg aaagaaaagc tggctggcaa
      301 caagacagcc gctgctcgcg acctgctcgc ggtggccgat gaactggtca acaagagcgt
      361 ttggatcatc ggcggcgatg gctggggcta cgacatcgga ttcggcgggc tcgatcacgt
      421 gctggcgtcc gacgccaacg tgaatatcct gctgctcgat accgaggttt actccaacac
      481 cggcggacag atgtcgaagt caacgccgtt aggcgccgtg gcaaagtttg ctgcgggtgg
      541 caagcgcact cccaagaaag atattgccat gatggcgatg aactacgggc gcgcctatgt
      601 ggcccacatc gcgatgggag gaagcgacgg gcagaccatg aaggcgtttg ccgaggcgga
      661 ggcgcacaac gggccttcgc tgatcatcgc ctacagccac tgcatcgcgc acggatacga
      721 catggtgcat ggcatcgagc aacagaagaa cgctgtgctg tccggacact ggccactgtt
      781 ccgctataac ccggggctgg ccaaggaagg caagaacccc ttccagcttg attcgcgggc
      841 gccgagcatc ccgttggaga agtacatcta caacgaagcg cgatacacca tgctggtcag
      901 cagcgacccg gaagaagcca agctcttgct gcaccaggcg caggacaacg ttaacgagcg
      961 ctggaaactg taccagcaca tggccgctat gagcttcgag aagtagcgag gagagaaact
     1021 gatgattgac ctgactacca actatctagg gttgaaactt aagaatcccg ttgttgtctc
     1081 agcctcgcca ctgaccgaga aactcgagaa cttcgagcga ctgcgcgatg ccggggcctc
     1141 ggccatcgtc atgtactcgc tgttcgagga gcaaatcgag gccgagagcg agaacctcga
     1201 cgccgcgatg gagctcggca ccaacagcta tgccgaagcc acaacctacc ttccggacat
     1261 gccgaaatac caaatcggcc ccgaccgcta catcgacttg atctacaagg cgaaaaagac
     1321 ggtcgacgtg cccatcatcg ccagcctgaa cggcaattcc cgcggcgggt ggacccgcta
     1381 cgcggagcac atggagcagg cgggagccga tgccctggag ctcaacatct tcaacattcc
     1441 aactgatccc tcggtgacgg cggagcagtt ggagcatggc tattgcgaac tggtgcgcgc
     1501 ggtgcgcaaa gcggtgaaga tcccggtctc ggtgaaactc gggccctact tcacttcgat
     1561 ggcaaatttt gtcaagcagt tgtcggctgc cggagccgat ggtgtcgtta tcttcaaccg
     1621 cttctatcaa cccgacttcg atctcgagga actggaggtc acgcccaacc tggtgctgag
     1681 cagtcctcac gagctgcgcc tgcggctgca ctgggcggcg atcctgtatc accaggtcga
     1741 aacctacatc gccatcacgg gcggagtaca tgactccacc gacgtgctga agtcgatgat
     1801 ggcgggcgca cacgtggcca tgatgacctc ggcgctgctc aagtacggca tcgagcacat
     1861 cggggtggtg ctgtccggca tggaacgctg gatggaagag cacgagtacc agtcggtcaa
     1921 gcagatgcgc ggcagcatga gcctggtgaa tatcgagaac ccgacggcat tcctgcgtgg
     1981 gaattatctc aagatgctgg gctcgtacaa gttcggcgag gccggttcag cgctaccgac
     2041 gtactaacgg tgtattcctt cgtctgaacg acagtggccg ggtccccgac gggagtccgg
     2101 ccattttttc agcatgttgg tgggccagaa ccagaggagg tgactccggc tgaagccggc
     2161 ttgctttctt gggttgcgct gcaacgcggc gctgaagcgc cgcactaccg gaacacggag
     2221 atgcagcaat ggcgaagccg ttccagattg ccaggattac ggcttcagta cgatcaccag
     2281 cgtgcggccg tcggtcttga tctcaccgcc ggtggccgtc ttggtttcct taatctgctt
     2341 gggacgatcc atgtatccca gggcctgcac cattccggct tgctgcaggg tggaagcaat
     2401 gaagtcccgc tcgccgtcaa ccgccggatc gatcttgtgg gtgatggcgt tttctttgcg
     2461 ctggtccttc tcaatgccga tgtcgtgcgt gcccgcaccg gcccaaagcg tctgcccgtt
     2521 gggtcccttg aacggagtct gccagatgcg gaagtggtcg cgctcgccga tgactttcac
     2581 ggcctccgcg cgagcaaagc cgtaatcctg ccctcgtcca aagaggtaga gcgtgctcat
     2641 ggggaccgta acgtaggctt gcttctggag cgtcgcgatt agcgcactga ctacggcatc
     2701 ctgattggtt ttgtcaggaa gcatccagcc cgcggttttg aaagcgttgg tgacttgctt
     2761 ttcgctgcct accaggacga aattcaccag atcgccctcg tttccgccgg gagcggcctg
     2821 gtcggtcacc cggtacggga gcttctgatc gatgatcgcg aacaggggcc tgaaatcgta
     2881 gttggtcgga ttggcggccg ccgcgggggc tgcggtgcgg tcgatgtgga cctcaaactt
     2941 accggcggtc ggcttggaag tttggtcctg gttcacaccc agatataacc gtcccgtcgt
     3001 gggtgaggtt atggtggcgt cggcacccac caggaatgga gttgcagcgc ggtcgttgcc
     3061 gacctggccc accagcgcgc cgcgtccagc gctggagaca ctgagcgcgc gaagagtatc
     3121 tgcccatccg cgctgagcgc ctgcgggacc gactccagtc ttgtcgctga aatcgacagt
     3181 tcccgtggcg gtgatatgca gcttgtcacc gggattcacg tccatgccgg tgtcgatcca
     3241 gagactgtct ccgttgatcg aaacgtcgac gctctttgcg gtggcggtgg ctgcggccgg
     3301 tggtggcgca gccgacaagg aattcgcgca ccagaaagga aagatgagga ttgcggcaat
     3361 aaaaaacgac tgggctcgct tcatcgattt ctccctcctc ctctggcaaa gaagtataaa
     3421 catcgcgtct gctcaaggtt gcgaatccga cgcagatcac agggtagccc cgccgatggc
     3481 tatttttccc agatacatcg ggctgaccgg gcacttgctg gaaatctagt acggttttgc
     3541 cgcgaggtaa aagaattctt acggcgtcga ctaggctaaa cgatcgtgac cctctggtcg
     3601 cagccttcgg cgtcgccgca gacgttgcag acgcgacgaa cggtgcgaaa tacggtcaac
     3661 gtgattccgg gggtgcggta gtgcgacttg actggctcat cgcgtagcag gtcggtgctg
     3721 aggtatttct tgttgtcgtc ggcaaacacg gtcgcgacaa cggcgtcatc acccagatcg
     3781 tcctgcgcct tcaccgccgc gaggaaattg gctcctgagc tgataccgac cgcgataccc
     3841 aactcgcagg ccagtttctg cgccatcaga atggaatcgc cgtcactcac gttaatcaca
     3901 ggatcgaggt catccagttg caaaatggcg gggacaaact cgtcggagat gccttgaatg
     3961 cgatggtgac ccagcttgca gccggtggag agggtcggtg actcggacgg ttccatggga
     4021 taaacgcgag ttgcagggtt ctgttgcttc agatagcggc cgacgcccat caccgtgccg
     4081 ccggtaccga cgccggcgac gaaagcatct gcagtcagcc cttccgactt gagttgcaag
     4141 tacagttctg gtcccgtcgt ctgggcgtgc gcttcgacat tggcagtgtt ggaaaactgt
     4201 cgcggcagga agacgtcttg gcgttccatc gccatttctt ccgacatgag aatgcttccg
     4261 aggaacccgc cttgctgctt ggtcaccaga atgatgtttg cgcccaggct gtggatcagg
     4321 tccatgcgct cgcggctcat ccagttgggc atgaagatgg tcacggggtg gcccagcgcg
     4381 cgtcccacgg cggcaaaggc gatgccagtg ttgccgctgg tggcttcggc gatggtgtcg
     4441 ccggggtgga gccgtccctc ggcgtaggcc ctgcggatga tgtgcagtgc catccggtcc
     4501 ttgatgctgc cggtgaggtt catctgctcg ctcttggcgt agatggtgcg cttgcggcct
     4561 gagacttcgt actcgatggc aagcaggggc gtgttgccga tcagtctctc aagtgcgcgc
     4621 agtcggtctg cgatggccag agttgtaaca ggagcagtgg tgagcataaa agtaatgtcc
     4681 cgaaaaacgt ggaacaactc tcagtttgcg ggttacaggg ggtgagccgc gagaatagct
     4741 gtcacagatg gcagtgacag acgtcataag cgggtaatcc gagttgcctg tgtctcactg
     4801 atccgcggga caccgttgac tatccccgcc gggggaacag gaacttcagc atgggcccaa
     4861 cgcgttcggc ccaggcgtac tcgctgtgtt ggccgtgttc gatttcgcga tattcgaggt
     4921 cgatgccctt cttccagcct ttttggagca ggagtcgctt cagcgcgcgc gcgtcgcgaa
     4981 gtgcccgctt accttcgctg gtacccatgt cgagccagat gcgctgccca gtgttgcgac
     5041 ggatttccgc gatcgtgcgc agaattgcgc ggttatgcca ccacaccgac ggagacatga
     5101 tcgccaaatt gccgaagata tcgggataac gcacgcccag atataacgag accaggccgc
     5161 ccagagaaga gccgcccatg ccgcagtttt ctgcgccggc gagcgtccgg tactggtgtg
     5221 ccacgaaggg cttgagttcc tccaccagca tctggccgta ggcgtcggca cggccgccac
     5281 ccaggcgctt gtcttcgaca ggagtgtact cgtcgatccg cttcacaccc gtgttgtaga
     5341 tgccgacgat caccagcggt tcgacctcac ccgcgtcgat cagcgcatcg gcggtctcgc
     5401 ctacacgcca gtagtttccc ttgatgaacg aggtttccgg gtcgaacagg ttctggccgt
     5461 cgtgcagata gagcaccggg aagaaccggt tggtatcgtg acgaaagact tgcgggacgt
     5521 acacgatgaa gtcgtgtttc tcgtcgatca gacgcgaggc aaactgctca tgcttatgaa
     5581 gccgcgattg caggctgctg ggcgcggagg attggatcat tgctggggcc ggaatgtggg
     5641 catcgtgggc gataacgggt tcggtcgaca ttcttgtaat attgaaatat cacacgccgc
     5701 ccgggaagca gagcaatgct actttagttc ccgctggtta tgcgaagtgg ttccttagag
     5761 tagcaattcg gttttcgatt cagcaaatca ttccacaaca ggggcgcaca tcgtgcatcg
     5821 tgaatttcat aattggtggt ccaaagacct gcaacgggac atgtcactgc tctgctttgg
     5881 ccacgccggc cgcaaagtcc tggtgtttcc gacgtctcgc gggcgttttt ttgagtacga
     5941 agacaacaag atgattgagg ccgtcggcga caagctcaat aacggcgaat tgcagattta
     6001 ttgcgtggac agcgtcgacg acgagagctg gtacaacaaa tccgcgcatc cctactggcg
     6061 cttgcagcgc cacctgcagt acgagcgcta catcgtcaac gatgtcttcc cgctgatggg
     6121 gaacaagaac tggactcccc acaccacggt caccggttgc agctttggcg cgtatcacgc
     6181 cgtgaacttc gcgttccggc atccggacat ggtcaacggc tgcgtcagca tggggggagc
     6241 gttcgacatc cggcagttcc tggacggctg gtacagcgag gatgtgtatt tcaacaaccc
     6301 gcccgactat ctggccaact gcgctgacgg gtggaagtac aaccacatgc ggacggtgct
     6361 cgcgacggga gagcacgaca tgtgctggga ccgcaacgag aagtttgccg ggattttgcg
     6421 ggcgaagggc atccggcatg aaattcacgt gtggggcgat agaagctatc acgattggcc
     6481 atggtggcgg ccgatggcgc gggtgtatct gtgagaacga cagcccttgc cgacgagatt
     6541 tgaagggttc cgacgcggcc cgctccacgc aggattgggc cgattttggt tcgcaaaggc
     6601 gcctgttttc ccctacaata atggttactt ttgagcgcac cccgcgctga ttcccgggca
     6661 cttccagaga gttcttgcga gcggatacag cgggggattt gtgtcgtttc caaaggagcg
     6721 gtacgtgaag aaaatcggag tcatcttcgg gatggaaacc acgtttccac ctgccctggt
     6781 agacaaaatc aatttcatga acgagccggg tatccaggcg gagctggcca cggtcgggga
     6841 agttcgcatg gcggagccgt ctccctacca cgtcataatt gaccgcatct cgcacgacat
     6901 ccccttttac cgcgcctatc tcaagaacgc cgctctttcg ggcgcgatcg tgatcaacaa
     6961 tccgttttgg tggagcgctg acgacaagtt cttcaacttc gcgctgggcg aaaagctggg
     7021 cgtggcaatt ccccagactg tcctgctgcc gcacaagcag catccgcccg gtaccactga
     7081 ccgctcgatg cgcaatctgc agttccctct tccctgggac agcattttca gttatgtagg
     7141 atttccggcg ttcctgaagc ccttcgatgg cggcggctgg aaggatgttt acaaggtcaa
     7201 taacccggaa gaactgttca aggcctacga ccagagccgc gatctctgca tgaccctgca
     7261 acatggagtg aagttcaagg aatactttag gtgttatgtg gtaggccagg aaaaagtaca
     7321 catcatgcac tacgatccat cggcgccttt ccacgagcgc tacgtgaaga acccaccacc
     7381 tgtggatcca aggttgctgg agcgcgtgga acgcgatgcg cttacactct gccgcgcgtt
     7441 gggttacgat ctcaataccg tggagtttgc ctgcgaggac ggcgttccgt atgccatcga
     7501 cttcatgaac cctgcgcccg acgccgacat caattccgtg ggcgaggaca acttccactg
     7561 gatcgtagac gccgtggctg aactggcggt tcgcaaggcc aaacagcaca ccggcaacgt
     7621 gtcggaatat cgctggagcg ccttcctggg cggcaacccc gcagaggtga agtcggaagc
     7681 cacagcgaag gatgcaaaag ccgtgaaagc tccaaagagc gctaccaagg ggtaatgtag
     7741 atgaagcggc cgagcctcag cataggaatc gaagaggaat accagacgat cgatccggag
     7801 acactcgatc tccgctcgca cattcagtcg gagattcttc cgaaagccat cctgtcgact
     7861 aattcgcggg ccaaggcgga gatgcaccag gcggtcatcg aggttgggac caaggtctgc
     7921 cgcgacatcc aggaagcccg gaccgacatg ctcgaactgc gccgcgagat gatttcgctg
     7981 gcaaacgcaa atggcctgtt gctgggcgcc gccagcactc atcccttcgc cgattggaag
     8041 gcgcaggaga tttatcccga cgagcgctac cgccaggtgg tcgaagacat gcaactggtg
     8101 gcgcgtgcca acctggtttt cggcctgcac gtgcatgtag gcatcgaaga ccgcaacacg
     8161 gcgattcata tcatgaattc gctgcgatat ttcctgccgc acatcctggc gctgtccacc
     8221 aattcgccgt tctggatggg catgcagacc gggttcaagt cgtatcgctg caaagtattc
     8281 gagcgcttcc ctcgcaccaa tattcccgac gtctacgcca actgggcgga gtacgaaacg
     8341 ttcatcaact tgttgatcaa gacccactgc atcgacaatg gcaagaaggt ctggtgggac
     8401 atccggccgc atccgttctt cgacacgctc gaggtccggg tgtgcgacat tcccatgcgc
     8461 ttggatgaaa ccctggcgat tgccgccctg atccaggcca cggttgcgat gctgtggaac
     8521 ctgcacgcca cgaacaagag ttaccgcatc tatagccgcg cgctgatcat ggagaataag
     8581 tttcgcgcct cccgttacgg cctcgacgga aagctcatcg actttggcct ggaagaggaa
     8641 gtaccaacgc gagaactgat gctggaatac ctcaactgga tcgatccggt gctggatgaa
     8701 ctgggctcgc gcgaggagat aaactacatt caccagatgc tggcgatggg caccggcgcc
     8761 gaccgccaac tgcgggtctt ccaggaaacc ggggacctga ggaaggttgt tgaatacatc
     8821 gttgccgaga cccgggttgg ggtgtttgac tccgagccgg cgcatcgcta aaaagttcgc
     8881 cactgaccct ggcctgacca gaaaggacgc tacttgacac caccattgct gaccgatctg
     8941 acgaccacgc ccttcgaccc cgagcttacg ccgggcgcat tcaacgcagt gcacacttgc
     9001 ctgcggattc agcccgacga aaaggtcacc gtaatcacgg acaatgcctg ccgcgacatc
     9061 gccgccagta tcgcccagga actggtcaag gtcggttcgc ccttcaaggc ctttgtactg
     9121 gaagaaatcg ccgaacgtcc gctgaccggt ctaccgcgcg acatcgccga cgatatggaa
     9181 accagccagg tcagcatctt cgcagtgaac gtgcagcgca atgagctgaa gtcgcgcatg
     9241 gagatgaccg acatcgtcaa tcgccgccgc atgcgccacg cccacatggt caacatcaat
     9301 cacgagatca tgctggaagg catgcgggcc gacttcaatc gcgtcgatgc gctcagcaag
     9361 aaagtcatgg agatcgtgac tcaggccaag caagtacacg gcaagaatcc cgcgggcagc
     9421 gacctggtgg ctgatctcaa ccccaactat aagtggatca agaccagcgg catcatcagc
     9481 ccggataagt ggggcaa
//
LOCUS       DASRKK010000081         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_297837, whole genome shotgun sequence.
ACCESSION   DASRKK010000081 DASRKK010000000
VERSION     DASRKK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297837"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            790..>2322
                     /locus_tag="VFC15_01685"
     CDS             790..>2322
                     /locus_tag="VFC15_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HZM08900.1"
                     /translation="MRVNDKGYLDTQGFSLFLYDSTYHPVFVDQKNTAMELIFHGQRV
                     ATNGDVRLLPTPEQWDLVATLKGRQADKANNRLTADLSFPTFDLSYTLEVEAEPGGVK
                     VSINLDKPLPPKLAGRAGFNLEFLPSIYMGKTYLVDRTKAGIFPRTPNDPMEKAMPLA
                     DEPKKAYYLEDWEKAKGYTQPRPFAEGKSITLGVDDALARVNVTSDTADLMLFDGRNR
                     AQNGWFVLRSLIPSGKTTGAIIWHIRPDVIPNWTRPPMIAHSQVGYAPGFSKVAVLEL
                     DPKYNGPKTAQVLRLMEDGTYKQAFEGPITPATPWLRYVYSKFDFTPVKDPGLYVIEY
                     ADQRTAPFPIAKDAYANIWQDSLDHHLAAQMDHVSVREGYRVWHGASHLDDGRLAPVV
                     GEQFDGWNQATATDGKYKGGDHIPGMNVGGWYDAGDFDLEEPAQLSVIQSLALAYREF
                     DLKYDELMVDEVAREVEMHRPDGVPDTVQQVKHGALLILAQFHNIGHAIRGTHEPDLR
                     QYT"
BASE COUNT          526 a          679 c          700 g          417 t
ORIGIN      
        1 agcaacgtac agccacagtt acagccacac ctcggcaagc cgctgacctt gagcgcaaac
       61 gttcttgaga tcgcagcagc ccaggttcca cgcgggagtt gtcgtaggga aagttccgca
      121 aacagggtgc cgagcacaat cgggtcggtc gtcacaatgg gcactttgta gtgcgaaacc
      181 tccttagtgt caaaacgcgc cgggacgacc gagacttatc agctgtaagt ccaaccaaag
      241 caacatttgg gagtcggaga actctgctcc cagtcaaagt gcgattggca aaataaaaat
      301 ggcgaggccg ggcgaacgga gcgccaatgc tgtctgaaat ccgagtctat gatacaaacg
      361 tcttcacctg tccctcgcca aaatccagcg cacgcataga atcagctgtg ctctgcaaca
      421 acctcttcca ggcgcaacgt gcgtaggtac tgaggaaaac gcacctgctc gccaaggacc
      481 cgaagggtag ggggcagaag tcgcgctcgg aggaaatgcg ggttacgagg cgctcattgc
      541 gcaccgatgt ccccagagat ggctcccggc cagctttctg ttggccgacg atccgggagt
      601 tgagaggtcc gtgaacacaa ggggcgttac cggcagtaat cctgtgaacc aacgtcgccc
      661 cgacggtgtg cgtgtcatat gatgtggagg caaaggagca gttaacccca gactatgctc
      721 accctagaca gagtgtgtct ccttatagtc cttatctcaa gcccaagtct gttcggcgcc
      781 gatttcgcca tgagggtgaa cgacaagggt tacctcgata ctcaaggttt tagcttattt
      841 ctctacgaca gcacgtacca tcccgttttc gtagaccaga agaacacagc catggagctg
      901 attttccacg gccagcgcgt cgccacgaac ggcgacgtgc gcctgctgcc aacgcccgag
      961 cagtgggacc tggtggcgac cctcaaaggg cgccaagcgg ataaggcgaa caatcggctc
     1021 accgccgacc tgtctttccc gacgttcgat ttgagctaca cactggaagt cgaagcggaa
     1081 ccaggcggcg tgaaggtcag catcaatctc gacaagccgc taccacctaa gctggccggg
     1141 cgagcgggct tcaacctgga gttcctgccc tcgatctaca tgggcaagac ttacctggtg
     1201 gaccgaacca aagccggcat cttcccgcga acgcctaacg atccgatgga gaaggcgatg
     1261 ccgctggctg atgaaccgaa gaaggcgtac tacctggagg actgggagaa ggccaaggga
     1321 tacactcagc cgcggccgtt cgccgagggt aaaagcatta ccctgggggt cgacgacgcg
     1381 ctggcccggg tcaacgttac gtcggatacg gcggacctca tgctgttcga tggacgcaac
     1441 cgcgcgcaga acggctggtt cgtgttgcgt tcgctgatcc cttccggcaa gaccaccggc
     1501 gcgatcatat ggcacatcag gccggacgtg attccgaact ggacgcggcc gccgatgatc
     1561 gcgcacagcc aggtgggtta tgcgccgggt ttctcgaagg tggcggtact ggaactcgac
     1621 ccgaagtaca acggcccgaa aacggcccaa gtgctgcgcc tgatggagga cggaacttac
     1681 aagcaggcgt tcgagggccc gatcacgcct gcgacgccgt ggctgcgtta cgtttattcg
     1741 aaattcgact tcacgccggt caaggacccc ggcctttatg tgatcgagta cgcagaccaa
     1801 cgtacggcgc ccttccccat cgccaaggac gcttacgcca atatttggca ggacagcctg
     1861 gaccatcacc tcgcggcgca gatggaccac gtttcggtgc gcgaaggcta ccgggtgtgg
     1921 cacggcgcat cgcacctgga tgatggccgc ctggcgcccg tggtcgggga gcaattcgac
     1981 gggtggaacc aggccacggc gacggacggg aagtataaag gcggcgatca cattcctggg
     2041 atgaacgtgg gcggctggta tgatgctggc gatttcgacc tcgaggagcc agcgcaactg
     2101 agcgtgattc agagtctggc cctggcgtat cgcgaattcg atctcaagta cgacgagctt
     2161 atggtggatg aagtcgcgcg cgaggttgag atgcaccgtc ccgatggcgt gcccgatacg
     2221 gtgcagcagg tcaagcacgg cgcactgctc atcctggcgc agttccacaa tatcggccac
     2281 gccatccgcg gcactcacga accagacctg cggcaataca cg
//
LOCUS       DASRKK010000082         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_884503, whole genome shotgun sequence.
ACCESSION   DASRKK010000082 DASRKK010000000
VERSION     DASRKK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884503"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VFC15_01690"
     CDS             complement(<1..1398)
                     /locus_tag="VFC15_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZM08901.1"
                     /translation="MSSVRLLTIVRGFLVALLCCTAAFASDFKVSVESPAGGRVSGVQ
                     VSLFRIGDNAGVGVQTTAGDGTATFPHLADGQYRLVVLAPGFAEQSLQVSLPQTQSLT
                     VELKLATTPQTVVVSATATPATAEQTGTSVGVLTSEQLNAINPVSAPDALLYIPGAIV
                     SSTGRRGNLASLFVRGGESNYNKVLVDGVPVNDPGGIFDFGVVPMNNMDRLEVERGPE
                     STVYGSDAMTSVVQLWTATGSTRTPEIQFGADGGNFSTAQGYASLAGARGIFDYNVFA
                     NQFNTQGQGVNDAYSNSLQGGNLGVRLSDRVAFRLRARHYNSWTGIQSNWWFNGNPEL
                     PPDPNQYAHQNNFLASGDLTISGPGAWQHQFSGFEYHHVALNVNPLDDLDRPFDSAFN
                     SLAKYNLAGFAYQGIYSPRPWAQTTLGYNFQDENGYINNNSDFGISTTHGLRFNNYLF
                     VQQTIVWRRLSILAGV"
     regulatory      complement(1471..1665)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1731..2444)
                     /locus_tag="VFC15_01695"
     CDS             complement(1731..2444)
                     /locus_tag="VFC15_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM08902.1"
                     /translation="MPNPTSAAAGEIHQFRLTLLQRMQLTLVSWIASAVVGLIGRTLR
                     MTITYEEGSIRGLDSIAPGIYPFWHRCVLPATWLFRNRDIGVLTSLSRDGEYIARVIR
                     RFGFVPVRGSSSRGGQRGLLEMETMVKAGGAVAFTIDGPRGPRYVAKKGPVTLARLTG
                     IPITAFYVAVEKAWVLKTWDAMLIPKPFSRAYVRVAKNIFVPPDADDAALDRYHAEMQ
                     ASLERITGFAESQFSARHD"
     gene            complement(2454..>3359)
                     /locus_tag="VFC15_01700"
     CDS             complement(2454..>3359)
                     /locus_tag="VFC15_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM08903.1"
                     /translation="VAQHKVTYLTRDAADFRGNRELRSAVTAALAKHRLAEKGMSGPE
                     VEALLTPITLDAVHIEKGKEGVSGAAIFATAVAMVMLLYAVVLIYGVAVMRSVIEEKS
                     SRIIEVLLSSVTAKELLAGKILGVGAVGLTQILIWIIFAVAFAVSGMMAAKSFVSEIH
                     IPTAGFIFFGVFFLLGYFLYSAMYAAIGSMVTTDQEAQQMQWPAMVPIILAVFLMNPV
                     IQQPNSQLAFWLSIIPFFAPILMLVRILVEEPPVWQLALCLGLMLATIYGLLSLASRI
                     YRVGILMYGKRPTLPELRRWLRYTG"
BASE COUNT          688 a          991 c         1048 g          632 t
ORIGIN      
        1 aacgcccgcc agaatggaca gccgccgcca cacgatggtc tgctgcacga acagatagtt
       61 gttgaagcgc aaaccgtggg tggtgctgat tccaaagtcc gaattgttgt tgatgtatcc
      121 gttctcgtct tgaaagttgt agcccagcgt ggtttgcgcc cacggccgcg ggctgtagat
      181 tccctggtag gcgaagccgg cgaggttgta cttggccagg ctgttgaaag cgctgtcgaa
      241 tggccggtca agatcgtcga ggggattcac gttaagcgcg acgtggtggt actcgaagcc
      301 gctgaactgg tgctgccagg cgcccggccc gctgatcgta agatcgccgc tggccaggaa
      361 attgttctgg tgcgcgtatt ggttcgggtc cggcggcagc tcgggattcc cgttgaacca
      421 ccagttcgac tgaatgcccg tccaactgtt gtagtggcgt gcgcgcaggc ggaatgccac
      481 gcgatcggaa agtcgcacgc ccaggtttcc gccttgcagc gagttcgaat aggcgtcgtt
      541 cactccctgg ccttgcgtgt tgaactggtt ggcgaagacg ttgtagtcga agattccgcg
      601 ggctcccgcc agcgaagcgt agccctgagc ggtggagaaa tttccgccgt cggctccgaa
      661 ttgaatctcc ggcgtgcgcg tgctgccggt cgcggtccaa agctgcacca cgctggtcat
      721 ggcatccgat ccgtataccg tgctctcggg accgcgttcc acctcgagcc ggtccatgtt
      781 gttcatggga accacgccga aatcgaagat gccgccggga tcgttcacgg gcacgccgtc
      841 caccagcacc ttgttgtaat tggattcccc gccgcgaacg aacaagctgg cgagattgcc
      901 gcgccgtccg gtgctgctga cgatggctcc gggaatgtag agcaaggcat caggggcgga
      961 aacgggattg atcgcgttca gttgctcgct ggtgagcact ccgaccgaag tacctgtctg
     1021 ctccgcggtg gctggcgttg cggtggcgga gacgaccacg gtttgcggcg tggttgcgag
     1081 cttcaattcg acggtcagcg actgcgtctg tggaagggaa acttgcagcg actgctcggc
     1141 gaatcccggt gcgagcacca ccagacgata ctgcccgtcg gcgagatgcg gaaacgttgc
     1201 tgtaccatcg ccggcggtgg tttgcactcc taccccggcg ttgtcgccga tgcgaaacaa
     1261 gctgacctgc acaccggaga cgcgcccgcc ggcagggctt tccactgaca ctttgaaatc
     1321 ggatgcgaag gctgctgtgc agcacagcag tgcgacgaga aatccacgaa caatggtcaa
     1381 gagacgaacg ctggacatga cactcttcct ttacacgcga aaggggttgg tgatcagggc
     1441 acccccatcg cacgcaaagt cggcgtgcgc cggggccccc gtagttcgcg ccggtctcct
     1501 ggcttgcgag ttgcgatttg catcgcggac gacggcggtg gccttcccgt tggaacagtg
     1561 gccgatccac caaagactcg cttacagttg cgcggcagcg cgggcttttc acccgcttcc
     1621 cagttccgcc ggtgagatga cggaacgcgc gaacacgaat tggcaaggaa caatcccgga
     1681 ccaagttggt tcaggatgag attcgactgt actgagagtg aaagcgggtg tcaatcgtga
     1741 cgtgcggaaa actgcgattc cgcgaagccg gtgatgcgct ccagcgaggc ttgcatctcg
     1801 gcgtgatagc gatccagcgc cgcatcatcg gcatcgggcg gcacgaagat gtttttcgcc
     1861 acccgcacat aggcgcgcga aaacggtttg ggaatgagca tggcgtccca ggtcttcaag
     1921 acccaggcct tctccacggc aacgtagaac gcagtgatgg ggatgccggt taggcgcgcc
     1981 agcgtcaccg ggcctttttt ggcgacgtag cgcggaccgc ggggaccatc aatggtgaaa
     2041 gctacggcgc ccccggcttt taccatcgtc tccatttcca gcagaccgcg ctggcctccg
     2101 cgcgagctgg acccgcgaac tggtacaaac ccgaagcggc gaatcacgcg cgcaatatat
     2161 tcaccgtcgc ggctgaggct ggtcagtact ccgatatcgc gattgcggaa cagccaagtc
     2221 gcgggcagca cacagcggtg ccagaaggga taaatgccgg gagcaatgct gtcgaggccg
     2281 cgaatggagc cctcttcgta ggtgatggtc atacgcagcg tccgcccgat gaggcccacc
     2341 accgcagacg cgatccacga taccagcgtg agctgcatcc gttgcagcag cgtgaggcgg
     2401 aactggtgga tctccccggc ggctgcgctg gttggattgg gcacgcgaga attctaacct
     2461 gtgtaccgca gccagcggcg caactcggga agagtgggcc gctttccata catcagaatg
     2521 ccgacccggt agatgcgcga cgcaagcgac agcaggccgt agatggtggc cagcatcaac
     2581 cccaggcaca acgccaactg ccataccggt ggctcctcga cgaggatgcg aaccagcatg
     2641 agaatgggcg cgaagaacgg aatgatggac agccagaagg cgagctgcga gtttggctgt
     2701 tgtatcacgg gattcatcaa gaataccgcc aggatgatgg gcaccatggc cggccactgc
     2761 atctgctgcg cttcctgatc ggtggtcacc atggaaccga tagcggcgta catggcgctg
     2821 tacaggaaat atcccagcag gaaaaacacg ccgaagaaaa tgaagccggc ggtcgggatg
     2881 tgaatctccg acacgaacga cttggctgcc atcatgcccg agacggcgaa cgccaccgcg
     2941 aagatgatcc aaatcaaaat ctgggtcagc ccaaccgcgc cgacgccgag gatttttccg
     3001 gccagcagtt ctttggcggt cacggacgag agcaacacct ctatgatgcg cgaacttttt
     3061 tcttcgatga ccgaacgcat cacggcaacc ccgtaaatca gtactacggc gtagagcagc
     3121 atcaccatgg cgacggcggt cgcgaatata gcggcgccgc tgacgccctc ttttcctttt
     3181 tcaatgtgca ccgcatcgag agtgatcggc gtcaagagcg cctctacttc cgggccgctc
     3241 atccccttct ccgcaagacg atgctttgcc aatgcggccg tcaccgcgct gcgcaattcg
     3301 cgatttcccc gaaaatcggc agcatccctg gtgaggtagg tgaccttgtg ctgcgccac
//
LOCUS       DASRKK010000083         6707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_493705, whole genome shotgun sequence.
ACCESSION   DASRKK010000083 DASRKK010000000
VERSION     DASRKK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493705"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..753)
                     /locus_tag="VFC15_01705"
     CDS             complement(58..753)
                     /locus_tag="VFC15_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZM08904.1"
                     /translation="MPQTPHIERHFTAGEVVRDVVIGMSDGLTVPFALAAGLSGAVDN
                     SHIVVVAGLAEIAAGSIAMGLGGYLAARSDAEHYESERLRETREVQEIPDEEALEVQK
                     VFADYGLSHDESVPVVQALTRKPKEWVDFMMRFELGLEKPNPKRAMQSALTIAASYVC
                     GGIIPLAPYMAAHSARMALVWSVVVTLIALGVFGYIKGRFTGSRPGRSASQTVVIGGL
                     AAGAAFLLAKLIS"
     gene            complement(731..1255)
                     /locus_tag="VFC15_01710"
     CDS             complement(731..1255)
                     /locus_tag="VFC15_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZM08905.1"
                     /translation="MVREFSAGGVVLRKMRGRWFVAVIEPHMTRPKKSSKPSNRPQKT
                     AAALVALPKGAIDEGEKPQQTALREVSEETGVQANLVTKLADVKYYYVRNWGDRARVF
                     KIVSFYLLLYRSGRLGNIAPEMRIEVQNAYWLPLEDTPNTLSYLGEREVVQRALQYLK
                     AHPGLGDHATDTTH"
     gene            1526..2122
                     /locus_tag="VFC15_01715"
     CDS             1526..2122
                     /locus_tag="VFC15_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08906.1"
                     /translation="MANINYKTYALNAITPMKWWKTWIVRVFFFILGAVKPLQKDLVQ
                     LSFIHFARWVIIKRNAFPQASQQQPKEELKYDYLLFFSNFNGTWNQYIDAFSAVLSRG
                     LNSIWRWSEKFPGSVPVTPFKQYIARCQFDTDYYYTAYPYAATNDVKAAHRVQAALEA
                     FEKDSAGKSPEEFARSYLTFLVAVHGDLGETGKAPVGT"
     gene            2142..2789
                     /locus_tag="VFC15_01720"
     CDS             2142..2789
                     /locus_tag="VFC15_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08907.1"
                     /translation="MANQSGNVYGLTILSPILQDPNAETSHNCAIRMKLAMLPRDESS
                     PFARVSSTHMARLAVMDDVVYVGMPSCEEHLKSQYLVFETNFDGDLDTYLARMAREIP
                     TEVDGIWQHCWGHPGVQDVGAFIAYMKKCQITTTFYFADVNDKTVQQTLKALQTQAAV
                     AEFIEKNQGGTPAELQKSFADFLEMLRKAPPPIPGRPGTGGPPRIETKMKWATKN"
     gene            2815..4200
                     /locus_tag="VFC15_01725"
     CDS             2815..4200
                     /locus_tag="VFC15_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HZM08908.1"
                     /translation="MSKLDATDIQGFVLRGYGLPFARYMFLEIADAHSGQVFIGRLLD
                     QITTGERWDAGKPEWTLNVAFTHKGLVNLKLPDASLLSFPVEFVQGMKARGEILYDTG
                     ENGPEKWDAVWREGQVDIWLAVNSKTKETLDAVCADLRRLMGETGGAKVIDWQDAGAF
                     FIDGHWSAKEHFGYTDGFGNPDFLGVERDTQPGQGKLTADGTWAPLATGELLLGYADE
                     AGELPVAPIPHLLASNGTFMVYRKMHQNVATFRRYLEEKGKLYSGGKEKLASKFIGRW
                     RDGTPVELSPDAPNQEIVQDKQRNVNFTFGNDLNGTRCPIGSHVRRMNPRDAFGFNGR
                     LINRRRITRRGLPYGPYVPEGQPVVDDVEHGIIFMALNASLFRQFEFVQQQWVEYGND
                     ARLGNEKDMLMGNHEERDRYTIQGTEDPKNPPFVCGGMPNFVQLRGGDYFFLPSMTAL
                     RMIAAGTVDCR"
     gene            4621..5166
                     /locus_tag="VFC15_01730"
     CDS             4621..5166
                     /locus_tag="VFC15_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM08909.1"
                     /translation="MSKRYYSPRQLLRVCQELLAAFSPANDASPFDDVLKLLYDHRKY
                     FWIGIYFVLGDKVVRQAMQGPMPPCHVFEFGKGNVGTTGQSGLVKVIPDVTADATYSM
                     CFLEVKSEIVVPIRIGGRTLGVIDVESDCPNNFGSLDRMLLEGVAAMLARFLTSRGKV
                     LVRHARERQDALPKARHATAC"
     gene            5577..>6707
                     /locus_tag="VFC15_01735"
     CDS             5577..>6707
                     /locus_tag="VFC15_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HZM08910.1"
                     /translation="MIRFFVAVLGCAFCCSPLVLSQQKSQAEPFTPPTKIFGFRDPGP
                     ELKAEKTFLAVPDPQLAKEHLQFLTSAPHVAGSPEDLKTAQYVLDKYKAAGLDAYIQQ
                     YKVWMNLPLDIKVEVVAPKGVIMTGPSPERVSGDPYQDDPRILPAFNEFSPSGDVTAD
                     VVYANYGRLEDFQKLQEMGVDVKGKIVIVRYGENFRGVKAFVAQQYGAAGVIIYSDPW
                     DDGYFKGDKYPKGPWRPDTGVQRGSIQYLFRYPGDPTTPGIASVPDLPDSKRIAPELA
                     TDMPKIPTTPLSYADATPIMANLAGPESPREWQGALPFTYHVGPGPVKVHMALKQNYQ
                     FVTIYDVIGMVRGSRLPDEWVITGNHRDAWVYGAVDPNSGTAA"
BASE COUNT         1429 a         1929 c         1976 g         1373 t
ORIGIN      
        1 tgaatgagtc ctcattcagt caacaagaat ctgcataaat tcttttccca tattgggtta
       61 ggaaatcaat ttggccaaca ggaaggctgc tccggcagca agtccgccaa tcacaacagt
      121 ttgggaggcg gaacggcctg gcctcgatcc cgtaaagcgc cccttgatat agccgaaaac
      181 tccgagcgcg atcaaggtga ccacgacgga ccagactaga gccatacgcg cactgtgcgc
      241 cgccatgtaa ggcgccagcg ggatgatgcc tccgcagacg tacgaagccg cgatggttag
      301 cgcgctctgc atggcccgct tgggattggg cttttccaga cccaactcaa agcgcatcat
      361 gaagtccacc cactccttcg gctttcgcgt cagcgcctgc accacgggaa cgctttcgtc
      421 gtgcgacaag ccgtaatcgg cgaagacctt ctgcacttcg agcgcctcct cgtcggggat
      481 ctcctgcacc tcgcgcgtct cgcgcagccg ctcgctttcg tagtgctcgg catcgcttct
      541 ggcggccagg tagccaccca gtcccatggc gatggagccg gcggcgatct cggccagtcc
      601 ggcgaccacc acgatgtggc tgttatcgac cgcgccggag agacccgctg ccagcgcgaa
      661 gggcacggtg agcccgtccg acatgccgat gacgacgtcg cggacaactt cgcccgcggt
      721 gaagtggcgc tcaatgtgtg gtgtctgtgg catggtctcc taaccccgga tgggctttca
      781 gatattgcaa cgcacgctgg accacttcgc gctcgccgag atagctcaag gtattggggg
      841 tgtcctccag cgggagccag taggcgttct gcacctcaat ccgcatttcg ggcgcaatgt
      901 ttcccagccg ccctgagcgg tagagcagca gatagaagct gacgatcttg aacacccgcg
      961 cgcggtcgcc ccagttgcgc acgtagtagt acttgacatc ggccagcttg gtcaccaggt
     1021 ttgcctgtac tccggtttcc tcgctgacct cgcgaagggc cgtttgctgc ggcttctctc
     1081 cctcgtcgat ggcgcccttg ggcagggcca ccagtgcggc cgccgtcttt tgcgggcggt
     1141 tcgaaggctt gctcgacttc ttgggacgtg tcatgtgcgg ctcgatgacc gccacaaacc
     1201 agcggcctcg catcttccgc agcacgacac ctcccgccga gaattcgcgc accatgagcg
     1261 tccgccagag aaccggctag tttggcagac tccggggacg ctgacaatca aaagcgatgc
     1321 cgggtatagg tcatttcctg ggaagccgag tgacgggcca agtggcgtcg gatccaccgc
     1381 gcgccgatga acggcgaaat cggcgttgcc ctaggttcgt tgccgagatg aatttccaaa
     1441 tctggaagcc cccttgcgtt caccttgcat ttctaacttt gcaggctgat aatgtcggca
     1501 cttcgccgac ggaaccggaa gggccatggc caacattaac tacaaaacct atgcgttgaa
     1561 cgcgatcacg cccatgaagt ggtggaagac atggatcgtt cgcgtcttct ttttcatcct
     1621 gggggcagtt aaaccgttac aaaaggatct ggtccaacta tcgttcattc attttgcccg
     1681 gtgggtcatc attaaacgta atgcattccc tcaggcctcc caacagcagc cgaaggagga
     1741 gctgaaatac gattatctgc tgttcttcag taacttcaac ggaacgtgga accagtacat
     1801 tgacgcattc tcggccgtct tgagccgtgg attgaattcc atctggcgct ggagtgaaaa
     1861 gttccctggt tcggttccgg tgacgccgtt caagcaatac atagcgcgct gccagttcga
     1921 tactgattac tactacactg cctatccgta cgccgccacc aacgatgtga aggcggcaca
     1981 tcgggtacag gcggcgcttg aggcttttga aaaagacagt gcaggtaaat cgccggagga
     2041 gttcgcacgt tcttatctta cgttcctggt ggccgtccac ggtgatcttg gggagactgg
     2101 aaaggcgccg gtaggcacat agaaatagca aagaggacag catggcgaat caaagcggaa
     2161 acgtatatgg gctgacgatc ctttcgccta ttctgcagga tccaaatgcg gagacttccc
     2221 acaattgcgc aatccgaatg aagctggcca tgctgcctcg cgatgaatcc agcccgtttg
     2281 ccagagtgtc aagcacccac atggcgcgat tggcagtgat ggatgacgtg gtgtatgtgg
     2341 gcatgccttc ctgcgaggag caccttaagt cgcagtacct tgtcttcgag acgaattttg
     2401 acggtgatct cgatacctac ctggcccgca tggcgcgcga gatccccacg gaagtggatg
     2461 ggatatggca gcactgctgg ggccatccgg gagtacagga cgtcggggct tttatcgcct
     2521 atatgaagaa gtgccagatc accactacgt tctactttgc cgacgtaaac gacaaaacag
     2581 ttcagcagac gttgaaggcg ctccaaacgc aggccgcagt ggcggaattt attgaaaaaa
     2641 accaaggcgg gaccccggcc gagctgcaga aatcctttgc cgacttctta gaaatgttgc
     2701 gcaaagcgcc gccacccatt cccggtcgcc cgggaactgg cgggccgcct cgcatcgaga
     2761 caaaaatgaa gtgggcaacc aagaattagc caagacaggg gtgggcaacg ccaaatgagt
     2821 aaattggacg cgaccgatat tcaaggattc gtgttgcggg gatacggtct ccccttcgca
     2881 cgctatatgt ttctcgagat tgccgatgct cacagcgggc aggttttcat tgggcggctc
     2941 ctggatcaga tcaccaccgg cgaacgctgg gatgcgggca agcccgagtg gacgctgaac
     3001 gtcgccttca cccataaggg cctggtgaac ttgaagctac ccgatgcctc gcttctgagc
     3061 tttccggtgg aatttgtgca gggcatgaaa gcgcgaggcg agatccttta cgacacgggt
     3121 gagaacgggc ccgaaaagtg ggacgcagtc tggcgcgaag gccaagtcga catctggtta
     3181 gccgtgaatt ccaaaaccaa agagacgctg gatgcggtgt gtgccgacct gcgaagactg
     3241 atgggggaga caggcggggc caaggtcatc gactggcaag acgcgggagc attttttatc
     3301 gatggtcatt ggagtgccaa agagcatttc ggctacacag atggattcgg caatcccgat
     3361 ttccttggcg tggaacgtga cactcaaccc ggccaaggaa aactgaccgc cgatggcact
     3421 tgggctcccc ttgccacggg tgaacttcta ctgggatatg cggatgaagc gggcgaactc
     3481 cccgtggcgc cgatccctca cctgctcgcc agcaacggga cattcatggt gtaccgcaag
     3541 atgcaccaga acgtcgccac attccgccgg tatctggaag aaaagggaaa gctttactca
     3601 ggaggaaaag agaagctggc ttcgaagttt atcggccgct ggcgtgatgg gacgccagtc
     3661 gagctttcgc ccgacgcgcc caatcaagaa atcgtgcaag acaaacaacg gaacgtaaac
     3721 ttcaccttcg gtaacgacct gaatggcacg cgctgcccta ttggctcaca tgtgcggcgc
     3781 atgaatccgc gggacgcgtt cggcttcaac ggccggctga tcaaccgtcg ccgcatcacg
     3841 cggcgcggcc tgccatacgg gccgtacgtg ccggagggtc agccggtagt ggacgacgtc
     3901 gagcacggaa tcatttttat ggcgctcaac gcaagcctgt tccggcagtt tgagtttgtg
     3961 cagcagcagt gggtggaata cggcaacgac gctcggctcg gcaatgagaa agacatgctg
     4021 atgggcaacc acgaagaacg tgacaggtat acgatccagg gaacggaaga tcccaagaat
     4081 ccgccgtttg tgtgcggcgg tatgccgaac tttgtgcaac tgcgcggcgg agattatttt
     4141 ttcctgccca gcatgacggc gctgcgcatg atcgccgcgg gcacggtgga ttgtaggtaa
     4201 aagagcgatg agggcacgtg gaacgggtcg aaaacctcgt tctgcctcga atgaccgggt
     4261 actcggttcc agcaggtcga cggatcgcgg tgcctggcat tacgcgcgtg gacttgcgta
     4321 tgccagtaag cgcgaccgga agaatctgat cgccgagcgc gacagcctca ccgcgatagt
     4381 ggacaaactg ccggccggac atcaaggttc aattcgatcg ggcttgggcc catgccgacg
     4441 tgcagccgaa cctgcaatat ttctaggcct gcggccggca gtgacttggt tctcggtgat
     4501 taggaaacca aaactccgtg tcctccgtgt ctccgtggtg aatgattttc gcctgagcgc
     4561 aattggagtc tcgccctccc gcgactacac tggtggtgga cattttctca ggaccatttc
     4621 ttgtcaaaac gctattactc tcccagacaa ctactgaggg tttgccagga acttctggcc
     4681 gcattctcgc ctgctaacga tgcatcgccg ttcgacgacg tgctcaagct gctctacgat
     4741 catcgcaagt atttctggat tggcatttac tttgtgctgg gcgacaaagt ggtgcgacag
     4801 gcgatgcagg gaccgatgcc gccgtgccac gtgttcgagt ttggcaaagg gaacgtggga
     4861 accacgggac agagcggact ggtgaaagta atccccgacg tcaccgcgga tgcaacctac
     4921 agcatgtgct ttctggaggt gaaatcggag atcgtggtgc ccattcgcat aggcggacgc
     4981 acgctgggcg tcatcgatgt cgaaagcgat tgccccaaca attttggtag cttggatcgc
     5041 atgttgctgg aaggtgtcgc cgccatgctg gcgcgctttt tgaccagccg cggcaaggtg
     5101 ctggtgcggc acgcgcgcga acgccaggac gccctgccga aagcgcgcca cgccacggca
     5161 tgctagaaat tttaccttgc ggtggtggca acgggtggag caggccttca caggctccgg
     5221 aggaagccat gttcgggtag tgccgcgctt cagcgccgcg tcaacgagcc gacgggaaag
     5281 gggcctttag gccctgaggt acctaccgat ttcgctacga cgggcgtatt tcgcgaggat
     5341 tcgaagcgaa gtacctcagt ggctaaagcc ggatctcata cattccgctc aacgcgggcc
     5401 tgaaggcccg cactacccga gcagtgtgtg aacatttttc tgcagacttt caggcgtgcg
     5461 ttgtgggcag caatttccga tcggcgcctt cgcgacacgt tcccgatgat gtaagctgct
     5521 attccttgcg gctgtgctcg gaataagttc gccacaattt tcccgagggt atcgctttga
     5581 tccgtttctt tgttgctgtg ctcggttgcg ctttctgctg ttcaccgctg gttctcagtc
     5641 aacagaaatc tcaggctgag ccattcacac cgcctacaaa aatattcggg tttcgcgatc
     5701 ccggcccgga gttgaaagcg gagaagacct ttctggccgt acctgacccg cagttggcca
     5761 aggagcatct gcaattcctg acctccgcgc cccacgtcgc cggatcgccg gaagacctga
     5821 agacggccca gtacgtcctg gacaaataca aggctgccgg gctcgatgcc tacattcagc
     5881 aatacaaggt ctggatgaac ctgcctctcg acatcaaggt cgaggtggtt gcgcccaagg
     5941 gtgtcatcat gaccggtcca tcgcccgagc gggtcagcgg cgatccctat caggacgatc
     6001 cgcgcattct gcccgccttc aacgaattct cgccgtccgg cgacgtcacc gccgatgtgg
     6061 tgtacgccaa ctatggccgt cttgaggact tccagaagct gcaggagatg ggcgttgacg
     6121 tgaaggggaa gatcgtcatc gtccgctacg gcgaaaactt tcgcggcgtg aaagcgtttg
     6181 ttgcgcagca atacggagcc gcaggcgtca tcatctactc cgatccgtgg gatgacggct
     6241 acttcaaagg cgacaagtat cccaaggggc cgtggcgccc cgataccggg gtgcagcgcg
     6301 gttccatcca atacctgttc cgctatcccg gcgatcccac cacgccggga attgcgtccg
     6361 tgcccgatct gcccgacagc aagcgcatcg cgcccgagct cgccacggat atgccgaaga
     6421 ttccgaccac accgctctcc tatgccgacg ccactcccat catggccaac cttgctggac
     6481 cggagtcacc gcgcgagtgg caaggtgcgt tgcccttcac ctatcacgtc ggtccagggc
     6541 cggtgaaggt acacatggcg ctgaagcaga actatcagtt cgtcaccatc tacgacgtga
     6601 tcggcatggt gcggggttcg cgacttccgg atgaatgggt gatcaccggc aatcatcgcg
     6661 atgcctgggt gtacggcgcg gtggacccca acagcggcac cgccgcg
//
LOCUS       DASRKK010000084         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_689348, whole genome shotgun sequence.
ACCESSION   DASRKK010000084 DASRKK010000000
VERSION     DASRKK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689348"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFC15_01740"
     CDS             complement(<1..231)
                     /locus_tag="VFC15_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08911.1"
                     /translation="MRPALPAIRQEADNPTIKECLSITGGRALGCLGNDWLSGTGSNA
                     VADVVFRKRCPSSPWRKWRVFRKADDGAGYAIP"
     gene            711..2588
                     /locus_tag="VFC15_01745"
     CDS             711..2588
                     /locus_tag="VFC15_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM08912.1"
                     /translation="MNILDLVVGKPIQTSDERAEQIGPAQGIPIFGLDALSSAAYGPE
                     AALSLLIPLGLLGVRYIVPVSAAIITLLVIVYFSYRQTIAAYPSGGGSYTVARFNLGA
                     SASLLAAAALLADYILTAAVGISAGVGALVSAVPSLLPHTVSICVGILIVITILNLRG
                     VRDAGLAFAAPTYLFVGTLLITIVVGIFRVVVSGGHPTPVVAPAPPSPMTAVVSYWLL
                     LKVFASGCTALTGVEAVSNGVKAFREPAVKNAQRALTVIIFLLAALLAGISYLVKAYG
                     IVATDPGLPGYQSLLSMLSAAVFGKGFFYYLTMGSILVVLSLSANTAFADFPRLCRAI
                     AQNNYLPHVFGYRGRRLVYTYGIVVLAVLCGGLLILFGGVTDRLIPLYAVGAFLAFTL
                     SQSGMVRHWWKNRGPLWRRKVLVNGLGGFVTGVTVVVVLFAKFLQGAWITLLFIPLTI
                     VIFVLVRRHYHMVRLLTACKDPVDIEGLSQPPIAVIVIDRWSNITRQGIEFASRLSPE
                     VIALHVTITEYNEVLQVEWERYVEKPFRAAGKEPPQLHALPSPYRFVIVPIVQFILDL
                     SEKHPARNIIVVIPELVENKWYEYLLHNQRAHLLEWVLLARGNERIFAVSAPWYIGQQ
                     T"
BASE COUNT          559 a          907 c          851 g          716 t
ORIGIN      
        1 tggaatcgcg tatccggctc cgtcgtcagc cttgcggaaa acccgccact tgcgccacgg
       61 gctgctaggg caacgtttcc gaaagactac atccgccacg gcattacttc cagtgcccga
      121 tagccaatca ttgcccaaac aaccgagggc acgccctcca gtgatggaca gacattcttt
      181 gatcgtcgga ttgtcggctt cttgccggat cgccggcagg gccggtctca cgttcctctc
      241 cggaaatgtg attgttacga cgattcggcg gtgtgccgtg acggtcgccg gaaactcgag
      301 ttctggcttg atgctgcagc cctgcaccgg gtttgggatt tcacctggaa tcagtggaag
      361 cgactcctcc tgccactcga cgctgaccac gcgctcgcgt cacaccgcaa cggctggagt
      421 gaaaagctca cgaaacatgc tctcgcgagc agtagggaac cgactctccg aggtgcaaac
      481 gccagttcag accacaactc aaccctctga tggcatagcc ggtgacagaa gtctgctgca
      541 gttctcttct atgatcgacc cctcgcgcac gaacaccgaa actctcttga tcgaataatc
      601 ccaccaatgt tatcgtgggt cggctcaagg caattctccg atcatgggcg aagctcggtg
      661 cgtgtgtctt gtttttgtcg gcgtaaacag tccatgggag accattgctg atgaacatac
      721 tggatctcgt tgtcgggaag cccatccaga ccagcgacga acgtgccgaa cagatcggtc
      781 ctgcgcaggg gatcccgatt tttggcctcg acgcgcttag ctcagctgct tatggcccgg
      841 aagcggccct cagccttctg attcccctcg gccttctcgg cgtccgttat atcgttcccg
      901 tcagcgccgc catcatcacg ttgctggtga tcgtgtattt ctcttaccgc cagaccatcg
      961 ccgcctatcc atccggcggg ggctcctata cagtggcgcg cttcaacctt ggcgcatctg
     1021 ccagcttgct tgccgcagcc gcgcttctcg cggattacat cctcaccgcc gctgtgggga
     1081 tttccgccgg tgtgggcgcc ttagtctcgg ccgttccatc gctgctgccg cacacggttt
     1141 ccatctgtgt cggcattctg atcgtcatca ctatcctcaa tctccgcggc gtgcgcgatg
     1201 ccggccttgc ttttgcagcg cccacctacc tctttgtcgg cacgcttctc attaccatcg
     1261 tggtagggat tttccgggtt gtcgtgagcg gcgggcatcc cacaccggtc gttgcccctg
     1321 ctcctccgtc gcccatgacc gcagttgtca gttactggct attgctgaaa gtctttgcta
     1381 gcggttgcac ggcattgacc ggcgtcgaag cggtcagcaa cggcgtgaaa gcgttccgcg
     1441 aacccgctgt aaaaaacgcg cagcgcgctc tgaccgtgat tatctttctt ctggcggcgc
     1501 tgctggcggg catttcctat ctggtgaagg cttacggcat cgtggcgacc gatccgggcc
     1561 tgcccggcta ccagagcctt ctctccatgc ttagcgccgc cgttttcggc aaaggctttt
     1621 tctactacct gacgatgggt tcgattcttg tggtcttgtc gctttcagcc aacactgctt
     1681 tcgccgattt tccgcgtctc tgccgcgcca tcgcgcagaa caactacctc ccgcacgtct
     1741 tcgggtaccg cgggaggcgg ttggtctaca cctacggaat cgtcgtcctg gcggtactct
     1801 gtggcggcct gctcatcctg ttcggcggag tgacagaccg gctgattccg ctctatgcgg
     1861 ttggcgcctt tctggccttc acactgtcac aatccggcat ggtcaggcac tggtggaaga
     1921 atcgcggtcc gctttggcga aggaaagtac tcgttaacgg cttgggtggg ttcgtcaccg
     1981 gcgtcaccgt cgttgtcgtt ctatttgcga agttcctgca aggcgcatgg attactctgc
     2041 tgttcattcc gttgacgatt gtcatcttcg ttcttgtccg ccgccactac cacatggtca
     2101 ggctgctcac ggcctgcaag gatccggttg acatcgaagg tctcagccag cctcccatcg
     2161 ctgtcattgt catcgaccgc tggagcaata tcacccgcca gggaattgaa tttgcctccc
     2221 ggctctcccc ggaagtcatc gccttgcacg ttacgatcac cgagtacaac gaggtactgc
     2281 aggtggaatg ggaacgctac gttgagaaac ctttccgcgc tgccggcaag gagccgcccc
     2341 aactccatgc tttgccatct ccttaccgct tcgtcattgt tcccatcgtg caattcattc
     2401 tcgatctttc ggaaaaacat cctgctcgca atatcatcgt ggtcataccg gagctggtgg
     2461 aaaacaaatg gtacgaatac ctcctccaca atcagcgtgc ccatcttctg gaatgggtgc
     2521 ttctggcccg tggcaatgaa cgcatcttcg ccgtgagtgc gccttggtac atcgggcaac
     2581 agacatgaat tccattgaga gtggtggaag ggcaaagcag aagtagaggc tcacctgacg
     2641 aaaacagggt ttcgcctgaa tggactgtct gtggtcgtga aaagaacacc tccggaattc
     2701 cgaccaatga ttgtggatct gcggtctgcc aacccgaagt tggccagaaa ccttagtgct
     2761 gtatcagacg cgccccaaac acagtgagtg tgcaggtagg ctcggcaccg cgaggcgcgt
     2821 ccgatacagc ggtttgaagg aagccgcccc tggggaggcg attatgccga ttcaatcgtg
     2881 ttggggttag gcgagaagct tggggaacag ctactggagc cggcaagagc gaggcgagga
     2941 ttggttgggt gagagtttgc gtgtggtggg aacgggattt tgggtcgagg ttgggaacgg
     3001 accgctggtt tgggggcaag ccgggcgcac cac
//
LOCUS       DASRKK010000085         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_884723, whole genome shotgun sequence.
ACCESSION   DASRKK010000085 DASRKK010000000
VERSION     DASRKK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884723"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFC15_01750"
     CDS             <1..713
                     /locus_tag="VFC15_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM08913.1"
                     /translation="ASKRGDVTINDRKADVKIALQHGDVTLNDVAGAVNISLQKGSVR
                     ASKIAGDLDVEGHIDNVTIDEVAGAVHLNGDFFDDIRLSKIAKTVTFKSSRSDMQIAS
                     VPGDLEIAGDEVRGSDLSGPVRLITRSKDIHIENVSGDLQLETTNGDIEVHAADKLPL
                     GRISISSKRGDISLVLPSKAGFQVQANTSKGDITSEFDAVKIDESKGSARASGAVGNG
                     VSKLQISTDTGDIRITKG"
     gene            complement(922..1602)
                     /gene="cmk"
                     /locus_tag="VFC15_01755"
     CDS             complement(922..1602)
                     /gene="cmk"
                     /locus_tag="VFC15_01755"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZM08914.1"
                     /translation="MSTARKLVIAIDGPAGSGKSTIAARLAKKLGYVNLESGAMYRAL
                     ALKAMERQVSLDDGEALRQLADGTVIDLEPRGEGNRVLLDGRDVSQRIRDTDVTAAAS
                     RVSIHPRLREVMVGRQRELGLDGGVVMEGRDIGTAVFPNAGLKIFLDADESVRAQRRV
                     LQDGSHSPERAQRVLAELAARDQRDRTRLASPLVAAPDAVHIDTTHKSIDDVVAEVEQ
                     LVKRVDRR"
     gene            complement(1599..2594)
                     /locus_tag="VFC15_01760"
     CDS             complement(1599..2594)
                     /locus_tag="VFC15_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08915.1"
                     /translation="MQEQQLQELRREKWRLNGRPIRTLDEAREFIDSVGFCLLYPQRP
                     AVLAPTFVGAYAGSDEHLPARQMAFADPSACQAKDLMVRLLREKAAYEANVFPDNNFI
                     VSAAVFPYFYGLVGDRNPRQAPKAGARSEYSPLAVDAFHIIQKEGPISEHRLRELLGG
                     DISAAALDRASDELWAKLRITRVDYTQEKGVFWDVLFRWSPEAVKEGMHVSVAESLTA
                     LVSKYLDCVLAAQQEEVEHFFSNLIGRARVREAISALQAARELSFVHVAGRVLLQVSS
                     LEAPPPHVERRPKLQPRFTPHPPEPGKPLVQRRPRKSPSASPSRPVPPAKDTGKE"
     gene            complement(2737..>3558)
                     /locus_tag="VFC15_01765"
     CDS             complement(2737..>3558)
                     /locus_tag="VFC15_01765"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08916.1"
                     /translation="LQVTAADAAFALRPPEVPPFNARLQLEGSGFAVEGNAAVPVASF
                     GPQTFGGPVSGAAAMTRGENGCSAVLADDDAPAGLDSLSTLRPVGQIRDSFILAINPQ
                     GLWIIDQHVAHERILFEKICKLRLAESPEKQQLLMPLIVELTPGQMSVFAEICDELNC
                     NGFEVEPFGSRTIAVKTTAAGVTAGDVEHMLHEILDQFERDEQVLNLEKIRTSIAASI
                     ACHAAIKVNMPLEQNKMEWLLGELAKTDCPMSCPHGRPVVLRYSVKDIQRAFKRI"
BASE COUNT          744 a         1088 c         1054 g          672 t
ORIGIN      
        1 tggccagcaa acgcggcgat gtgaccatca acgaccgcaa ggccgatgta aagatcgcgt
       61 tgcagcatgg tgacgttacc ctgaatgatg tcgcaggcgc ggtgaacatt agcttgcaga
      121 agggttcggt acgcgcctcc aagattgctg gagacctcga tgtggaaggc cacatcgaca
      181 acgtaaccat cgatgaggtg gccggtgcgg tccatcttaa cggcgacttc ttcgacgata
      241 ttcgccttag caagatcgcc aaaaccgtga ccttcaagtc atcgcggtcg gacatgcaga
      301 tcgcttccgt gcccggcgat ctggaaatcg ccggtgacga ggtgcggggc agcgatctga
      361 gcggtcccgt gcgtctcatc acccggtcca aggacatcca catcgagaat gtctcgggtg
      421 acttgcagct tgagaccacg aatggcgaca ttgaagtaca cgcggccgac aaactgcccc
      481 tgggccggat cagcatttcg agcaaacgcg gcgacatctc actggtactg ccgtcgaagg
      541 cgggattcca ggtacaggcc aataccagca agggcgatat cacctccgag ttcgatgccg
      601 tgaagatcga cgaaagcaaa ggctcggcac gggcatccgg cgccgtcggc aacggtgtga
      661 gcaagctgca aatcagcacc gacaccggcg acatccggat aaccaaggga tagttttcgt
      721 tcaccacaga gacacagaga cacagaggca cggagaaatc cgtgcctttt gtgcgttagg
      781 gcatgctgtc atcccgaggg agggccgctt cggcccgctg aagcagcggg ctcgctccgg
      841 atgacaaacc ggtggttacc tttagctgat aactggaaac tcgaaactgg aaactggaaa
      901 ctcgaaactc gaaactcgaa actacctacg atccactctc ttcacgagct gctccacctc
      961 agcgaccaca tcgtcgatgc tcttgtgcgt ggtatcgatg tgcaccgcgt ccggcgcggc
     1021 gaccagcggc gatgccaaac gcgtgcggtc gcgctgatcg cgggccgcaa gctcggcgag
     1081 cacgcgctgc gccctctcgg gtgagtgcga tccgtcctgc aagacgcgcc gctgtgcgcg
     1141 aacgctttca tctgcatcga gaaagatctt caggccggcg ttaggaaaaa cggcggtgcc
     1201 gatgtcgcgg ccttccatca ccacgccgcc gtccagccct aactcgcgtt ggcgccctac
     1261 catcacctcg cgcaaccgcg gatggatgga aacgcgcgat gccgcggcgg tgacatccgt
     1321 gtcgcgtatg cgctgcgaaa catcgcgacc atccaacagc acgcgattgc cttcgccgcg
     1381 aggttcgagg tcgatcacgg tgccgtccgc caactgccgg agcgcttcgc catcgtccag
     1441 agagacctgc cgctccattg ccttcagcgc cagcgcgcga tacatggcgc cgctttccag
     1501 gttcacgtaa ccgagctttt tcgccaggcg cgcggcgatt gtgctcttgc ctgaaccggc
     1561 aggcccgtcg atggcgatca ccaacttgcg ggctgtactc attccttgcc ggtgtccttc
     1621 gcgggaggga ctggtctcga tggcgatgcc gatggggact ttctgggccg ccgctgtacc
     1681 aagggcttac cgggctcggg tgggtgcggg gtgaagcgcg gctggagttt gggcctacgc
     1741 tcaacatgcg ggggcggcgc ttccaaggaa gaaacctgca gcaatacgcg gccggcaacg
     1801 tggacgaaac tcaattcacg cgcagcttgg agcgcgctga tggcctcgcg gacccgcgcg
     1861 cgaccgatga ggttggagaa gaagtgctcc acctcttcct gctgggctgc caaaacacaa
     1921 tcgagatatt tcgagaccag cgccgtcagc gactctgcca ccgagacgtg catgccttct
     1981 ttaaccgcct cgggtgacca gcggaacagg acgtcccaga acacgccttt ctcctgggtg
     2041 taatcgacgc gggtaatgcg cagcttggcc cacaattcat ccgacgcgcg gtcaagagcc
     2101 gcggcggaga tgtcgccgcc cagcaactcg cgcagccggt gttcggagat cggtccttcc
     2161 ttctggataa tgtgaaacgc atccaccgcg agcggagaat attccgagcg tgcgcctgct
     2221 ttcggcgcct gccgcgggtt tcggtctccg accaatccgt aaaaataggg aaaaaccgct
     2281 gctgaaacga taaagttatt atccgggaag acgttcgcct cgtaggccgc tttctcgcgc
     2341 agcaggcgga ccatcaaatc cttcgcctgg catgcgctgg gatcggcaaa cgccatctgc
     2401 cgggcgggca gatgctcgtc gctgccggca tacgcgccca cgaaagttgg cgccagcact
     2461 gccggccgtt gcgggtaaag gaggcagaaa cctactgaat cgataaattc tcgcgcctcg
     2521 tccagcgtgc ggataggccg cccgttcaag cgccattttt cccggcgcag ctcctgtagt
     2581 tgctgttcct gcatagaagt cggcaattag caattagcaa ctagcgcgct actctgtttg
     2641 tccccaaatc cctaggagaa gaaccgcact gcagcaagca ctaaatgcta aatgctaaat
     2701 gctaaatgcc aagtgctgac tgctgagtgc tgattgctag atccgcttaa acgcccgctg
     2761 aatgtcctta acagaatacc gcagaacgac cgggcggcca tgcggacagc tcatggggca
     2821 atcggttttg gccaactcgc ctagcagcca ctccattttg ttctgctcca gcggcatgtt
     2881 gaccttgatg gccgcatggc acgcaatgct ggcggcgatg ctggttcgga tcttttccag
     2941 gttcagcacc tgttcgtcac gctcgaactg gtccaggatt tcatgcagca tgtgctcgac
     3001 gtcgcccgcc gtaactccgg ctgccgtggt cttgacggcg atggtgcgcg agccgaacgg
     3061 ttccacttca aagccgttgc agttcagttc gtcgcatatc tcggcgaaga ccgacatctg
     3121 gcccggagtc aactcgacga tgagcggcat cagcagttgc tgcttttcag gagactcggc
     3181 caagcgcagc ttgcagattt tttcgaacag aatgcgttcg tgcgcgacgt gctggtcaat
     3241 gatccacagg ccctgtggat tgatcgccag gatgaacgag tcgcgaatct ggccaacggg
     3301 acgaagcgtg cttagcgaat ccagcccggc aggcgcgtcg tcgtccgcaa ggacggcaga
     3361 gcaaccattt tcgccccgtg tcatggcggc agcgcccgat accggacctc caaacgtctg
     3421 cggaccaaag ctggccacgg gaacagccgc atttccttcg acggcaaagc cgctgccctc
     3481 taactgcaac cgcgcattga acggcgggac ctcgggcgga cgcaaggcaa acgcagcatc
     3541 ggcagcggtc acctgcaa
//
LOCUS       DASRKK010000086         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_5215, whole genome shotgun sequence.
ACCESSION   DASRKK010000086 DASRKK010000000
VERSION     DASRKK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5215"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1264)
                     /locus_tag="VFC15_01770"
     CDS             complement(158..1264)
                     /locus_tag="VFC15_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM08917.1"
                     /translation="MAGLFLRAVDSLGASFAHDTFRGYHATVKDFLNYLGAEYRQLCS
                     VEQLRRDPHILGWLVHLRSKTPPLVLGTHRNHLQRLHRILQELTWTEQIPELARLLRP
                     EDSPRPEHRLPRPLVAAQDQSIQQELLRRNDLASNFLLLLRHTGMRIGECADLSYDCL
                     RSNGPDQWAIHVPLGKLKTERMVPVDDFVCQRVQRLRFLRSLDPIPPDGFLLARPRGR
                     EMLLRELRGYLRQVVAAVGISTRIVPHQFRHTYATEMLRAGVSFPGVMNLLGHSTATM
                     TMRYLEITLPDLQREFQIARSQPRHLAPQPKTPSPARADLPGLLDSLHAAQHILEMFR
                     RALPEGPVRRRVDRLGNRLTKIASETRKLGTPGK"
     gene            complement(1291..2337)
                     /locus_tag="VFC15_01775"
     CDS             complement(1291..2337)
                     /locus_tag="VFC15_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM08918.1"
                     /translation="MTFHLVHQHSLDQARSPFHIVEHETGREVAWINRYLDREWVRRL
                     AETTLRSYALDLLHFLRWWISVHHTDAVTEAALTESTLLDYVRFQSGQQPRPAAASIN
                     RRIIVAERALRSEFPEAPSAFAPGFHQIYWRRSPLGFGRPRPALSRLRVRMPRRIIVP
                     LSVDEVARFWSSFRTSRDLAIVGLMLLQGLRSKEVLALHRDDLLLAESQIRVRGKGNK
                     IRFLPLAPETIELLDHYLRLERPDACGSALFVSLKGRARGVRMTPAGLRSLFRHHRLT
                     TGVTIANPHRFRHTFASDMIRAGVSLPALMQLMGHSQIQTTMFYIQLTPLDVYQQYAR
                     AVAQHIRPVPRSQP"
     gene            complement(2334..>3046)
                     /locus_tag="VFC15_01780"
     CDS             complement(2334..>3046)
                     /locus_tag="VFC15_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZM08919.1"
                     /translation="LRQWRGAEPLLHEVARFYRVQFHRHSKAVAYLYQRGIRSPALIE
                     HMRIGYAPGGCLRGWLTQLGYALPALREAGLVTGVGYDAYLRRVVFPLEGNLYGRSIS
                     PSAPPHRFLPGAKGGLYSWKQAQSYPEVILVEGLFDYAVLWQAGFHNVTCSLGTHLNA
                     RQFRQLCDAPRTVYLAFDADRNGSGQQAAQSLACRLREQSVNVRRVSLPEGHDPNSFF
                     VQGGDAQQFQLLLEAARP"
BASE COUNT          640 a          843 c         1029 g          534 t
ORIGIN      
        1 gacctagacc caacacggtt cacttaaggg tggcatcccg acgcttccca gatggccgtt
       61 tctgaggagt ttcaggcagg cgggcagttc cacgaacggg cgacacgcgc ccaggcggac
      121 tgtttccgag tgggcgactt aaccggccaa tccctgccta tttcccgggc gtgccgagtt
      181 tccgcgtttc cgatgcaatc ttggtgagcc ggttgccgag tcggtcgacg cggcggcgta
      241 cggggccttc cggaagcgct cggcggaaca tttccaggat gtgttgtgcg gcgtgcaggg
      301 agtcgagcaa accgggaaga tcggcgcgag caggagacgg agttttcggc tgtggtgcga
      361 gatgccgggg ttgtgaacgg gcgatctgaa actcccgctg caggtcgggc aaagtaatct
      421 cgagatagcg catggtcatg gtggccgtgc tgtgaccgag caggttcatc actccgggga
      481 agctcacacc ggcgcgaagc atctccgtgg catatgtgtg tcgaaactga tgaggaacga
      541 ttcgagtgct gattccgacg gcagcgacta cctggcgcag atagccgcgg agttcccgaa
      601 ggagcatttc tcgaccgcgc ggccgggcca gcagaaaacc atccggtggt atcgggtcaa
      661 gggatcgaag gaaacggagg cgctgaaccc gttgacaaac gaaatcgtcg accggaacca
      721 ttcgctcggt tttcaatttg ccgagcggga catgaatcgc ccattgatcg gggccgttgg
      781 aacgcaggca atcgtaggat agatcggcgc actcaccaat gcgcatgccg gtgtgccgaa
      841 ggaggagtag aaagttgctg gcgagatcgt tgcggcgcag cagttcctgt tggatgctct
      901 ggtcctgggc ggcaaccagt ggccggggca gacgatgttc ggggcgaggg ctgtcctcgg
      961 gccggagcaa acgcgccagt tcgggaatct gttcggtcca agtcagctcc tgcaggatgc
     1021 gatgcagccg ttgcagatgg ttgcggtgag tgcccagaac caacggtggg gttttggagc
     1081 gcaaatgaac gagccagccg aggatatgtg gatcacggcg taactgttcc acagagcaaa
     1141 gctgccgata ttcggcgccg agatagttca ggaagtcttt tacggtggca tggtagccac
     1201 ggaatgtatc gtgggcaaac gatgcgccga gcgagtcgac ggcccgcagg aagagacctg
     1261 ccatggggtg tcggagtggg cggcggcgct tcatggctga gaccgaggga cgggccggat
     1321 atgctgggca acggcgcgcg catactgctg atagacatca agcggagtga gttgaatgta
     1381 gaacatggtg gtttggatct gcgagtgccc catgagctgc atcagtgccg gaaggctcac
     1441 gcccgcccgt atcatgtcgg aagcaaacgt atggcggaat cggtgtggat tggcgatcgt
     1501 gacacccgtg gtgagacggt gatgacgaaa cagggatcgc aaacctgcgg gcgtcatccg
     1561 aacgccacgc gcgcgtcctt tcagcgagac aaacagagcg gagccacagg catccggacg
     1621 ttccagccgc agataatgat ccagcagttc aatggtctcc ggagccagcg ggaggaagcg
     1681 gattttgttg cctttgccgc ggacgcggat ctgcgactcg gccagcagca ggtcgtcccg
     1741 atggagagcc agtacttcct tggaccgcag gccctggaga agcatcaggc cgacgatagc
     1801 caagtcccgc gaggtgcgaa aactggacca gaaccgcgcg acttcgtcca cggacagcgg
     1861 cacaatgatc cgcctgggca ttctcacccg cagccggctc agtgcggggc gagggcgccc
     1921 aaaacccaag ggagatcttc gccagtaaat ctggtggaag ccgggtgcaa acgcgctcgg
     1981 ggcttcgggg aactcgcttc gcaaggcgcg ctcggcgacg ataatccggc ggttgatgct
     2041 ggccgccgca ggcctgggct gctggccgga ttggaatcgc acatagtcca gcagggtcga
     2101 ctcggtgagt gcggcttcgg taaccgcgtc ggtgtggtga acactgatcc accagcggag
     2161 gaaatgcagc aggtcgaggg cgtagctacg gagcgtcgtt tcggccagac ggcgaaccca
     2221 ctcgcggtcc aggtagcgat tgatccaagc cacctcgcgg cccgtttcgt gctcgacaat
     2281 atgaaacggg cttcgtgcct ggtccaaact gtgctgatga acgagatgga aggtcatgga
     2341 cgggcggcct ccaacagaag ctgaaactgt tgggcatcgc ctccctggac gaagaagctg
     2401 ttgggatcat ggccctcggg cagcgagact ctacgaacgt tcacgctctg ctctctgagc
     2461 cggcaggcaa gtgattgcgc tgcctgttgg ccgctgccgt tgcggtcggc atcaaaggcc
     2521 agatagacgg ttcggggagc gtcgcagagt tgccggaact ggcgcgcatt gagatgggtt
     2581 cccagcgagc aggtaacgtt gtgaaagccg gcctgccaca acacggcgta gtcgaacaga
     2641 ccctcgacca gaatcacttc cggataggat tgggcttgct tccaggagta caagccgccc
     2701 ttggcgccgg gcagaaaccg gtgagggggc gccgaaggcg agatactgcg gccatagaga
     2761 ttgccctcca acggaaagac aacacgacga aggtatgcgt cgtagcctac accggtcacc
     2821 agcccggcct ctcgcagagc cggcaaagcg tatcccaact gtgtcagcca accccgcaga
     2881 caaccgccag gtgcgtaacc gatccgcatg tgctcgatca gcgccgggga gcggattccg
     2941 cgttggtaca gataggcgac cgccttgctg tggcggtgga actgcacgcg atagaaacgt
     3001 gccacttcat gcaacaaagg ctcggcacca cgccattggc gcagcc
//
LOCUS       DASRKK010000087         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_102924, whole genome shotgun sequence.
ACCESSION   DASRKK010000087 DASRKK010000000
VERSION     DASRKK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102924"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFC15_01785"
     CDS             <1..786
                     /locus_tag="VFC15_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08920.1"
                     /translation="LEYRYATSEPNTRASKGFDSAPADFSEAGPRMTMVDGEPLLENA
                     HHHEVSLSQRAGDNKLQIAYFNDRIKDPALLGVGDIAFDAGDILPDVYSGTFSFNGGA
                     LEAQGVRLVFQRRIADGLTATVDYAYGGVLELEQPNVDWSVVRSSLQHGWRHSAALKL
                     NGSIPRSKTRWIASYRWTSGDNTLTPVDLFNASAGQTDAFFNLFVRQPLPRMRFMPGN
                     MEALVDLRNLLAQGYVPVIGPDGKTVYLMQSARSVRGGVAFTF"
     gene            complement(858..1271)
                     /locus_tag="VFC15_01790"
     CDS             complement(858..1271)
                     /locus_tag="VFC15_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZM08921.1"
                     /translation="MKFHTEYLTFNTRKHREYIHITPQVEEALRKSGVQEGMVLISAM
                     HITAGVYVNDNESGLIRDIDKWLEELAPFREDYQHHQTGESNGDSHLKSLLVHHQVTV
                     PVTAGKLDLGPWQRVFYAEFDGQRRKRLIIKVMGE"
     gene            1369..>2498
                     /locus_tag="VFC15_01795"
     CDS             1369..>2498
                     /locus_tag="VFC15_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZM08922.1"
                     /translation="MTDSKQSEELQQLRQQMAELTRRVFRLEQLLRTGITVESEAEKS
                     APSRVERGQPEPLPTVAQVSPTLSDSRQPAVPPAFREPQFTSPVPSPKTEVSLETRIG
                     SQWLNRVGIVAVLVGLSYFLKLAIENNWIGPATRVAIGIVAGIGLILWSERFRTRAFT
                     AFAYSLKAIGIGALYLSLWAAFQFYRLVPATVAFFAMVLVTASSAARSLAQDSELLAA
                     FALVGGFLTPVLVSTNQNHEVILFSYVALLDLGTVWVVAIRGWQRLLFASFVGTTLLF
                     AAWASSYYTEPQLATTLAFASFFFLLYAGAPFLGRKPAMERRYPNVLVVLALLTASSY
                     FAASYLMLGEHYRFELAWLTVAVAVLFFVLTRALQTREASAHP"
BASE COUNT          451 a          783 c          694 g          570 t
ORIGIN      
        1 ctcgagtatc gctacgctac cagcgaaccc aacacccgtg ccagtaaggg tttcgattcg
       61 gctccggcgg acttcagcga agccggtcct cgcatgacca tggtcgatgg cgagccactg
      121 ctggagaatg cgcatcacca cgaggtctcc ctgtcgcaac gtgccgggga caacaaactc
      181 cagatcgcct actttaacga tcgcatcaag gatcccgctt tgcttggtgt cggcgacatc
      241 gccttcgacg cgggcgacat tttgccggac gtctactccg ggaccttcag cttcaatgga
      301 ggcgcgcttg aggcccaggg cgtgcgcttg gtgtttcagc gcaggattgc cgatgggctt
      361 accgcaaccg tggattacgc ctacggaggc gttctcgagt tggagcagcc caacgtagat
      421 tggagtgtgg tacgcagcag tcttcaacat ggctggcggc actcggctgc tctcaagcta
      481 aatggctcca tcccgcgctc caagacacga tggatcgcgt cctatcgctg gacgagcggt
      541 gacaacaccc tgacgccagt tgatcttttc aacgcttcgg cgggacaaac cgacgctttc
      601 tttaacctct tcgtgcgtca accgttgcca cggatgcgtt tcatgccggg taacatggaa
      661 gcgctggtgg acctccgcaa cttgctggca caaggctacg tgccggtcat cggcccggac
      721 ggtaagaccg tgtacctgat gcagtcggcc cgcagcgtcc gcggcggagt ggcattcacc
      781 ttctaaagtg atatttttgg caatgaagag gcccgctgca tgagcgggct ttttcaatgc
      841 cctgctttct ccgcaagcta ctcgcccatc accttgatga tcagccgctt tctgcgctgc
      901 ccgtcaaact cggcataaaa gacacgctgc cacggcccta aatccaattt gcccgccgtt
      961 accggcacag taacctggtg atgtacgagc agcgacttca aatgcgagtc accgttgctt
     1021 tccccggttt ggtgatgctg gtaatcctca cgaaacggcg ccaactcctc caaccacttg
     1081 tcaatatcgc gaatgagacc gctttcgttg tcattgacat acacgcccgc cgtaatatgc
     1141 atggccgaaa tcaggaccat gccctcctgg acgccgctct tgcgcaaggc ctcttccacc
     1201 tgtggcgtga tgtgaatgta ttcgcgatgt ttcctggtgt tgaacgttag atattccgta
     1261 tggaatttca tgccaccgct ctccgtgcgg gtacttacca acgctttcca gcgctttgcc
     1321 tggattttga ctttcgcgta ttgtaagccc agccatcggt gccacagcgt gacggattcc
     1381 aagcaatccg aagaactgca acaattgaga cagcagatgg cggagctcac gcgacgagtg
     1441 ttccgcctcg agcagttgct gcggaccggt attaccgtcg aaagtgaagc agaaaagagc
     1501 gcgccttcgc gcgtggaacg cgggcagcca gaaccattgc ccaccgtggc tcaggtctcg
     1561 cccaccctga gcgactctcg ccaaccggcg gtcccgccag ctttccgtga gccgcagttc
     1621 acctctccgg ttccctcgcc gaaaaccgaa gtttcgctgg agacccgcat cggtagccag
     1681 tggctgaacc gcgtcggcat cgtcgccgtg ctggtcgggc tgtcgtactt cctcaagctc
     1741 gccatcgaaa acaattggat cgggccggcc acccgcgtcg ctatcggcat agtggcaggc
     1801 attgggctga tcctctggag cgaacgtttt cggacgcgcg ccttcacggc tttcgcttat
     1861 tccctgaagg ccatcggcat tggcgcgctc tacctgtcgc tgtgggcggc cttccagttc
     1921 tatcggcttg tacccgccac ggtggctttc ttcgccatgg tgctggtcac cgcaagctca
     1981 gcggccaggt ccctggccca ggattccgag ctgctggctg ccttcgcgct ggttggaggg
     2041 tttctaactc cggtgctggt ttccaccaat caaaatcatg aggtcatcct gttttcttac
     2101 gtggcgctgc ttgaccttgg cacggtgtgg gtagtcgcca tccgaggttg gcaacggctg
     2161 ctgtttgcca gctttgttgg cacgacgttg ctcttcgcgg catgggcgtc cagttactac
     2221 accgagccgc agctcgcgac aactttggcc ttcgccagct tcttcttcct gctgtacgcg
     2281 ggagcgccct tcctcggacg caagccggcc atggaacggc ggtatcccaa cgttctggtt
     2341 gtactggcgc tgctcaccgc cagctcctac ttcgcggcca gctacctgat gctgggcgag
     2401 cactaccgct ttgaattggc ctggctcacg gttgcggttg cagtcctgtt cttcgtcctc
     2461 acccgggctt tgcaaacgcg tgaagcaagc gcccatcc
//
LOCUS       DASRKK010000088        19456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_5313, whole genome shotgun sequence.
ACCESSION   DASRKK010000088 DASRKK010000000
VERSION     DASRKK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19456
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5313"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(753..1907)
                     /locus_tag="VFC15_01800"
     CDS             complement(753..1907)
                     /locus_tag="VFC15_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZM08923.1"
                     /translation="MTKKQAGFATRSIHVGNEPDPSTGAVAPPIFATSTYAQEELGKT
                     KGYDYSRAGNPTRTRLEQNLASLEGGVAARVFSSGMAAVSALANLMQAGEHIVASNNL
                     YGGVPRLFNQVLANFGLEFTYVDTTDPRNVTNAIRKTTRFVYLETPTNPLMGLTDIAA
                     VSDIAHTRGCQVVVDNTFMSPYFQQPIKFGADMVLHSTTKFLNGHSDGIGGVLVCTTQ
                     DQSEKLAFVQKAVGAILSPFECWLVLRGVKTLAVRMKQHDQSGRVIADYLSHHRKVKR
                     VFYPGLKSHPQYELACRQMSGFGALITLETGSLANAKKLLKRVRICTLGESLGGVETL
                     ISHPATMTHAAIGAKGRKEIGLTDGMVRISVGIEDVEDLIADLDQALAGI"
     gene            complement(1984..2931)
                     /locus_tag="VFC15_01805"
     CDS             complement(1984..2931)
                     /locus_tag="VFC15_01805"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZM08924.1"
                     /translation="MKTAEMASLRVAEEITELVGETPVLHLRKIVPANVADVYAKLEY
                     INPGGSIKDRAALGMIQRAEKDGRLQPGATIFEATAGNTGVGLALIGCTRGYKVVLAV
                     PERFSQEKVILMEALGAEVIRTPHAEGMQGAIDLVKARAAKTPNSWVAAQFENQDNPG
                     FHYETTGREFYDQLQGRVDAVVIGAGTGGTFTGVARYFKERNPHVLAIAVETQGSILG
                     GGPKGEHEIEGIGASFIPATFDGTLADEIIAVSDHDGFDMVKKLAAQEGVLSGSSGGA
                     NVFVSIEIARRLGPGKRVATIIPDSAERYLSKDIFHFNG"
     gene            complement(3073..4053)
                     /locus_tag="VFC15_01810"
     CDS             complement(3073..4053)
                     /locus_tag="VFC15_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM08925.1"
                     /translation="MGTLTVGQTSTAPPQPKPVEPTPSAAQGQAPAEKSGDVFVFRKE
                     VDEVTLHATVVDDKHHLVTDLDRNSFTVFEDGQPQKITAFRREDIPVAMGIVIDNSGS
                     MRDKRQAVNAAAINFVKSSNPQDKVFVVNFNEDFFLDQEYTSSIPKLKDALERIEARG
                     GTALYDAVVASSDHLKKSGLLEKKVLLVVTDGEDNASRESLEQAIRRLQEENGPTVYT
                     IGLLGEEHSKRARRALRQMAEETGGIAFFPQSAAEVDSITQQIAHDIRNQYTIGYKPS
                     KPQSEGGFRTVKVDANAKGYKNLQVRTRSGYYAGQSRAASDQPADKAVAK"
     gene            complement(4088..5251)
                     /locus_tag="VFC15_01815"
     CDS             complement(4088..5251)
                     /locus_tag="VFC15_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM08926.1"
                     /translation="MARGGSNGVGSASSMIRPWVDFDAAGRFSFCPVHTLLCLRNSFA
                     VMVAVLLLSACSVVPAFAQNEQSDIHVVPREQKKDAKPDPTVMPPAIAAEAKSAGVSV
                     DPSLKTHTKPIRKDVDLVLVPVTVTDPMNRLVTGLEKDNFLLSDSGQPQEIKHFSSED
                     APISLGVIFDISGSMANKIDKSRDAVVEFFKTANPEDEFFLVTFSEKPEVLADFTTSI
                     EDIQSKLVYATPKGRTALLDAIYLGMNRMRKAKHEKKALLIISDGGDNHSRYTEAEIK
                     SMVKEADVQIYGIGLYDRDFKTPEEREGPQLLAEVSDVTGGRTFTIGNTNELADVATK
                     IGIELRNQYVLGYRPSNPSRDGKWRKIKVRLNPPKGLPPLHVYAKTGYYAPTE"
     gene            5461..6795
                     /locus_tag="VFC15_01820"
     CDS             5461..6795
                     /locus_tag="VFC15_01820"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZM08927.1"
                     /translation="MAKIYPFKALRYDPAKVSPAQVVTQPYDKISPAMQDRYYAASPY
                     NLVRVILGKAEPGDDERNNVYTRAAANLQHWRAEHVLARDAEPSIYLYTQTFNVPGDP
                     AGTQLERRGFIAAGQLEEYDKKVVFRHEQTLSKPKADRLNLLKATQSHFGQIFMLYSD
                     PAGKVDATLLQERTPDVEVRDEYDVVHRKWKVSDAATIQKVQASMDDKQLIIADGHHR
                     YETALNYRNEMRMRGDGDATAPYERLMMTFVNMDSPGLVVLPTHRVVFGLDSFDVAAM
                     NAKLGKYFDVEDLGATTDVKPPMERLRDAGKQGSALLAITGKGAFLLRSHPAQDSPAL
                     AGQSERQRGLDVVQLHKLVLEETLGMSEEDIRDQKHLKYVREAKEAAEEVGKGANVAF
                     LMNPVRMQQVRDIAFGGEVLPQKSTDFFPKLLSGLTIYSLEDDAQGTVTGGH"
     gene            6797..8254
                     /locus_tag="VFC15_01825"
     CDS             6797..8254
                     /locus_tag="VFC15_01825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZM08928.1"
                     /translation="MRLSHPRAVPIAATLLAIVLLGGAVHLLRLSTVQAQTPATQLAV
                     YSAQTSYTVPLLNLNGQPYVGLVELLEPLGTVDARPDGKKYKLKFTPPGAREMELQFQ
                     DGKDNGKVKGDKIKLPSIFAIQNSRGYVPLASVSELLSRALSTQIRLNPTARRLFIGN
                     IGERFTLDLRKGEPSKLFVSFDSAVNPTIATEPGHIRFTFRREPVIAAADHTAFTDPL
                     ITGANFSEHDGVAELDVTGNSALMANFADGGKTIIVTGALAPPPPAVAQLPIPQVNVA
                     PPVAQPPKPPAGPRFLVLIDPAHGGTEIGSAISPTLPEKDVVLALARKLQRELQSRGI
                     AAGLLRNADIAITLDQRAISANAARPAIYVALHAANSGKGVHVFTSLLPAESVSSRDF
                     LPWETAQSAYLDLSGSVAGSVSAELEARKLPNATLAAPLRPMNNIAAPAIAIEIASPS
                     NDVSEIASPAYLDQVAQSIAAGIAAVRGKIPEVRP"
     gene            8251..8841
                     /locus_tag="VFC15_01830"
     CDS             8251..8841
                     /locus_tag="VFC15_01830"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZM08929.1"
                     /translation="MSRPIVVMFIVLLLIAAGLGFYAYHLKAKVTAEEQRLAQQAQAV
                     VPAASGPTTLVTLYVASDEEGGLRRTQLNVALPQERSERARAILRALLGSYLQSGSQH
                     PIGAGADVRDVFLLNRDTAVVDTNVAFADAHPSGVLVEELTIASVVSTLNASDPQITR
                     VKILVDGKERDTLAGHADLRRFYLASGIGQVLKDTP"
     gene            8838..9674
                     /gene="murI"
                     /locus_tag="VFC15_01835"
     CDS             8838..9674
                     /gene="murI"
                     /locus_tag="VFC15_01835"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZM08930.1"
                     /translation="MTLPRIGVFDSGVGGLTVLKAMAEQIPAHYLYFGDTARLPYGTK
                     SADTVARYSIGAARFLEAQNIDLLVIACNTATALALPQIKQAARVPVIGVVEPGAAMA
                     AEVSQSRSAIVIATEATVSSHAYRDALRKHGIEAHEKACPLFVPLVEEGWIEHPVTEQ
                     VARIYLGEAMAQSSDTADVLVLACTHYPLIAPLLRRITPEHVKIVDSADSTARVVRRL
                     LSERSTQIESPERGNSRTAAASNHRSFRFFVTDSVEKFKRLATRFMGRQVDNVEHVDL
                     GG"
     gene            9715..10452
                     /gene="rph"
                     /locus_tag="VFC15_01840"
     CDS             9715..10452
                     /gene="rph"
                     /locus_tag="VFC15_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZM08931.1"
                     /translation="MVYRSDNRTLEQMRPLNIIPGYISTAEGSCLIELGNTRVICTAT
                     IEDTVPQFLRNSGKGWVTAEYGMLPRATLTRSPREVTKGRASGRTHEIQRLIGRSLRA
                     VTDMDKFGERTIFIDCDVIQADGGTRTASITGAFVAMGLAMTAMVETGALSAAPIRDY
                     VAATSVGIVDGEIMLDLCYEEDSRADVDMNLVVTGSKKIVEVQATAEHRPFDDAQLAQ
                     MMALAKKGIESLVARQQAVLTGLPLRQ"
     gene            10981..12777
                     /locus_tag="VFC15_01845"
     CDS             10981..12777
                     /locus_tag="VFC15_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08932.1"
                     /translation="MKTTLVLALAVALSLLLTASVFAQSSSSSAMKEMKDAIAAQQQQ
                     IQQLQQQIQSRDSAIQTLEQRVSQAEAAATQAQSTAKSAIGGEKKEGALSAEEFNALQ
                     HDVTDLKAVSANTVEGLQDTQKRVGDLESPLAIHFKGITLTPGGFLAGETVWRPHAAL
                     GFATPFSAIPFSAAAQARSSEFYGSGSQSRLSMLAQGKWNTTNLTGYYESDFLGSAVT
                     SNANQTNSYVLRQRQLWGQAGLANGWTFTGGQMWSLVTETKKGEDNRSEATPMQIDPN
                     YTAGFSWARQFAFRATKNVNNKVWFGVSVENAQTILASSGAAANYVIGGPGVGAGLYN
                     QFANYSTNESPDFVAKIAFEPGFGHYEVFGLASDFRDRIYPNATAAKPSAVGAYNSSI
                     WGGGWGANARWLAYQKHIELGLHFLGGKGVGRYGDTQLADVTVDPNGNLKALQNYQAL
                     VSLEWHSPKWDFYGYGGGDYVGKTWYLNSAGKPEGYGSPLFNNTGCGTETVPGTSQPF
                     YATTAGYEPGALASCVGENKSVIEGTTGFWYKPYNGPKGRLQIGVQYSYLVRTAWVGY
                     GLPPYNNLPGPATLAPKAIENMVFTSFRYYLP"
     gene            13159..14157
                     /locus_tag="VFC15_01850"
     CDS             13159..14157
                     /locus_tag="VFC15_01850"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HZM08933.1"
                     /translation="MKLTPGKLAGMKAVSNENGVIAAAAMDQRGSLKKALGTRATDEG
                     LIEFKNYVTEVLTQHASAILLDPEWGLGPSKLRAKGSGLLLAYEKTGYDKALSGRLPD
                     LLDVWSVRRLKEAGADCVKILLYYSTFDDAKINDHKQAWTERIGDECRANDIPFFLEL
                     IGYKEGLEDKGPEYAKLKPQVVADSMAEFSTDRYGVDVLKVEVPVNLQYVEGAKSFKG
                     PKVYTKAEALQYFRNAAKATNKPFIYLSAGVSNPEFIEDLEFAQEAGVKFNGVLCGRA
                     TWKDGIPIFADKGGKAFEAWLKDEGVKNIENVNNALKAATPWYSIYEVPEPALATK"
     gene            complement(14238..14684)
                     /locus_tag="VFC15_01855"
     CDS             complement(14238..14684)
                     /locus_tag="VFC15_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZM08934.1"
                     /translation="MPFPESAAPGSLQNALLSRGIRLTRQRRIILEIIETAKQHLDAA
                     QILRKASKVDPDINRVTVYRTLSLLKRHGLVDELDLLHVKGEGHFYERRPQRDHMHMT
                     CLRCGKVQEFESDLFDRVKGQVERDCQFHIVISRFEIGGYCTHCRK"
     gene            15005..15529
                     /locus_tag="VFC15_01860"
     CDS             15005..15529
                     /locus_tag="VFC15_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08935.1"
                     /translation="MKGLTASPQDGNQFLSGVTKFEYGAKGWWTTEFYLDGQTTANQS
                     TIFTGFRWENRFRLLMHEHLINPVFYVACEDTNGADKSLMEVVGFDSQYDGLVPNKVA
                     SRDRQGELETKLILSSNVNGWNIAENFIAEKNLAGEPWEFGYAVAVSRPLSLMASPEE
                     CTLCRENFQAGVEF"
     gene            15560..15736
                     /locus_tag="VFC15_01865"
     CDS             15560..15736
                     /locus_tag="VFC15_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08936.1"
                     /translation="MHGTSQYVAPVLSWQVGNTVFKVSPTFGLTDASYRAMLRFGVQY
                     EISGLRRKLEKLFH"
     gene            15772..16173
                     /locus_tag="VFC15_01870"
     CDS             15772..16173
                     /locus_tag="VFC15_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM08937.1"
                     /translation="MNRSLRLLIVSTAFLTTAAIFCMAPHLAAQTFHNAPASAAQMKN
                     PYAGDAQAAAGGKKLYAQNCAQCHGNNLQGMGPAPTLLGPKVKDAKSGELFWFITNGN
                     LNNGMPSWSQLPKPQRWQIVTFLESRNSAGQ"
     gene            16227..17033
                     /locus_tag="VFC15_01875"
     CDS             16227..17033
                     /locus_tag="VFC15_01875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM08938.1"
                     /translation="MSTSNPFFAPKTALITGASSGIGLELSHLFARDGYRLVLVARNR
                     STLRQLGDDLQARYSITVRIEPKDLAHPATPSELYQELQESGIVLDVLVNNAGFGGAG
                     AFVDTDWNHEGEMMQVNIVALTHLTKLFLPQIRAREGMIMNVASTAAFQPGPFMAVYY
                     ASKAYVLSFTEALAEELRGTGVKVSCLCPGPVETNFQKRAYLEGTAMANSPLMVDVRE
                     VARIGYEGLKQGKRIVIPGWKNRVGVELLRISPREMVTKVVRKIQEKKSG"
     gene            complement(17075..17845)
                     /locus_tag="VFC15_01880"
     CDS             complement(17075..17845)
                     /locus_tag="VFC15_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZM08939.1"
                     /translation="MSISAVIVDDEQLARDELAYLLKEVGVDVVAQGKNGVEAVNLIK
                     DLSPDLVFLDVQMPGLDGFGVIKKLLDRKVPMPQIVFATAFDQYAVKAFEVNAVDYLL
                     KPFDKKRVAQSVEKARKKLESTASPNDQIGTLVKMLEQQQRPQAAKVLLKSVGRLFLV
                     DQKDICYASIEDGIITVVATNMEGQSNCRTLEELLDSLDPNVFWRAHRSYLVNINRIK
                     EVVPWFKSSYQIRMDDKKQAEIPVSRAQTKRLRELFNL"
     gene            17953..18714
                     /gene="rlmB"
                     /locus_tag="VFC15_01885"
     CDS             17953..18714
                     /gene="rlmB"
                     /locus_tag="VFC15_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZM08940.1"
                     /translation="MVVIFGIHAVSEALKSGARSFDYVGIARERHDQRVQRLIEDCHT
                     SGVQVRFLPREELDRTANSNTHQGVIAVTSSKQYADVDTLIEQRHGEYSFLVVLDGIE
                     DPHNLGALIRTADGAGADGVIIPERRAVGLTGTVVKASAGASEHLAIARVTNISRTLE
                     ELKDRNVWTVGLDERGTKSYDELDYKMHCAIVLGAEGKGLHDLVRKRCDFLVSIPMLG
                     QVASLNVSVAGAVVMYEVARQRRSPKSQGKAKPSK"
     gene            18711..>19456
                     /locus_tag="VFC15_01890"
     CDS             18711..>19456
                     /locus_tag="VFC15_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08941.1"
                     /translation="MKRWLTLFVCSLAMACTAAALDREAFTVTRYQLDVQIDRASHVM
                     AVTGQLTLRNDSKSPQKYATLQVSSSLSWNGIVLDRTPVEWIGNNYTSDIDHTGGLSE
                     AIVTLPKDVPPGGTVNLDVQYGGTVTPDSTRLTRMGAPAEMALRNDWDQISEPFTAMR
                     GLGYVAWYPVSLPAVSMSDGNAVFDAIGAWQFRQQRTEFDARIHVISGDAKLCIVGNA
                     TTSSCGEMGGTENPRTGGRVGQITNSIHLNG"
BASE COUNT         4079 a         5554 c         5736 g         4087 t
ORIGIN      
        1 accagtccaa acacgcagaa cagcaggccg atcagcagca ggttgtgccc cgtcatgccg
       61 agaaaggtga ccgaactcag atcgggaagc tgcaggttcg cttcgccagc ggtttcagtc
      121 ggctgggcca aggcggctga ggcattcagc atgagaacag tcagagttgc gccgattacc
      181 ataaccgaac gcgatatgga tccaggtatt aatgagagcc gccgcaaggt ggcgagccag
      241 ctttgcataa aagtcctccg atgggttacg cccggttcga ttcgggatcg ccccatgaaa
      301 tatccgcaca gaattgcgtt ctcacgcgac cgtccgcgat gagtttcagc atatgaccca
      361 tgagcatcca gcaggttagg ccactacggt cttcggcgtt gcgttccgat cgagcgcgac
      421 tgaaaaccat aggttgacct gctcaaatcc cggaaaacgc gcttccactt caagacagaa
      481 gtgatgaggt ttggccgcgt cgggaatact tgcattcgtc ctccaggcag gcattgccca
      541 gattaagaac gcaacacatg aagcgagtct ttataacacg ttgcgaaaag tcgggtcaat
      601 cgcatgcgtc actgagcagt gtgtcatccc gaaaaacctc tgaaaatggg gcgacaatga
      661 gacgttcact ccgcaagaac gcgaccaccg cgaattaacc agggaaattc ggattacatg
      721 acccacccaa gcggaccgat tccgaatcgg ctttagatcc ccgcgagcgc ctggtcgagg
      781 tcagcgatca ggtcttcgac atcttcgatt ccgactgaaa ttcgcaccat gccatcagtc
      841 aggccgatct ccttgcggcc tttggcgccg atggcagcgt gggtcatcgt ggcaggatgc
      901 gagatcaggg tctcgacgcc tcccaaggac tcgccgagag tacaaatccg gacccgcttc
      961 aggagttttt tggcgttggc aagtgaaccg gtctctaacg tgatcaatgc gccgaagccc
     1021 gacatctggc ggcacgccaa ttcatattga ggatggctct tgagaccggg atagaagacc
     1081 cgcttcacct tgcgatgatg gctgagatag tcggcgatga cgcgtccgct ctggtcgtgc
     1141 tgcttcatgc ggactgccag cgtcttgaca ccgcgcaata ccagccaaca ttcgaacggc
     1201 gacagaattg ccccgaccgc cttctgcacg aacgccagct tctcagactg atcttgcgtg
     1261 gtgcaaacca gtacgccgcc gatcccgtcg ctatgaccat tgaggaactt cgtagttgaa
     1321 tgcagcacca tgtcagcgcc aaactttatg ggctgctgga agtatggaga catgaaagtg
     1381 ttgtccacca ccacctggca gccgcgcgta tgtgcgatgt cgctgaccgc ggcgatgtcg
     1441 gtcagtccca tcagcggatt ggtgggcgtc tcgaggtaca cgaagcgcgt ggtcttgcgg
     1501 atagcgttgg tcacgttgcg cggatcggtg gtatcgacgt aggtgaactc cagtccgaaa
     1561 ttcgccagca cctggttgaa caggcgaggc acgccgccat agagattgtt cgacgcgaca
     1621 atgtgctcgc cggcttgcat gaggttcgcc agcgccgaga ccgcagccat tccgctggag
     1681 aacacgcgcg ccgcgacgcc gccttccagc gatgccaggt tctgctcaag ccgcgtccgt
     1741 gtagggttcc cggcacgaga gtagtcgtat cccttggttt tgcccagctc ttcctgcgca
     1801 taggtcgagg tggcgaaaat cggaggcgcg accgctccgg tggacgggtc aggctcattg
     1861 ccaacatgga tcgagcgcgt ggcgaaaccg gcttgctttt tggtcatgga tttttattag
     1921 ccttgttctg aagaggctgc tgctatccgc gtcctgaagg tatgtcaact cgagggttcc
     1981 gagctatccg ttgaaatgaa atatgtcctt ggagagatat ctctccgccg aatctggaat
     2041 gatggttgcg acccgcttgc ctggacccag ccttctggcg atttcaatcg acacgaacac
     2101 gttggctccg ccactcgatc ctgacagcac gccttcctgc gcggcaagct tcttcaccat
     2161 gtcaaaaccg tcgtgatcgc tgaccgcgat gatctcgtcc gcgagtgtgc catcgaaggt
     2221 cgcggggatg aagctggcgc caataccttc gatctcgtgt tcccctttcg ggccaccgcc
     2281 gagaatagac ccctgggttt cgacggcaat cgccagcaca tgcggattgc gttccttgaa
     2341 atagcgcgcc acgccggtga aggtgccgcc ggtaccggca ccgatcacca cggcgtcaac
     2401 ccgtccctgc aattgatcgt agaattcgcg gcctgtggtt tcgtagtgga agccgggatt
     2461 gtcctgattt tcgaactgcg cggcaaccca ggaattgggg gtcttggcag cgcgtgcttt
     2521 caccagatcg atcgcgccct gcattccctc tgcgtgaggg gtgcgaatca cctcagcacc
     2581 cagcgcctcc atgagaatga ccttttcctg ggaaaaccgc tctgggacgg ccagcacaac
     2641 cttgtagccg cgagtgcagc cgatgagggc cagtccgacc ccggtgttgc cggcagtggc
     2701 ttcaaaaatc gtggcgcccg gttgcaggcg cccgtctttt tcggcgcgct gaatcatgcc
     2761 aagagcagcg cggtccttta tgctgccgcc ggggtttata tactcgagct tggcgtagac
     2821 gtccgcgacg ttagcgggga caattttgcg caggtggagg acaggagttt caccaaccag
     2881 ttcagtgatc tcctccgcca cccggagaga ggccatttca gcagttttca tgagcacctt
     2941 gatgttaacg gtgttgactg acaagggtac gtgatggcga ttgccgcggt caacgacgcc
     3001 ggtcccgtcg aagcctaaga atatcgtttg gcgccatccg aggggtaaat cgcgagctgc
     3061 tcttttcgct gattatttgg cgacagcttt gtccgccggt tgatccgagg ccgcccggct
     3121 ctggccggcg tagtacccac tgcgggttcg cacttggaga ttcttgtagc ctttcgcgtt
     3181 ggcgtccact ttgacggtgc gaaatccacc ttcgctctgc ggcttggacg gcttgtatcc
     3241 gatggtgtac tgattgcgga tgtcatgggc gatctgctgg gtgatggagt ccacttctgc
     3301 cgcactctgg gggaagaacg caatgccacc tgtttcctcg gccatttggc gcagggcacg
     3361 gcgcgcgcgc ttggagtgct cctcgcccag caatccgatg gtgtaaacgg ttggaccgtt
     3421 ttcctcttgc aggcgtcgaa tggcctgttc cagggactcg cggctggcgt tgtcttcgcc
     3481 atcggtgacc accagcagga ctttcttttc cagcaatcct gacttcttga ggtggtcgga
     3541 agatgcaact accgcgtcgt agagggcggt cccgccgcgg gcttcgatcc gctcgagcgc
     3601 gtccttaagc ttgggaatcg acgacgtgta ttcctggtcg aggaagaagt cttcgttgaa
     3661 attgacgacg aagaccttat cctgcgggtt gctggactta acgaaattga tcgcggctgc
     3721 attcacggcc tgacgtttat cacgcatgga tccggagttg tcgatcacga ttcccatggc
     3781 aaccggaatg tcttcgcgcc ggaaagcagt tatcttctgg ggctgaccat cttcgaaaac
     3841 cgtgaaagag ttgcggtcga ggtcggtgac caggtggtgc ttgtcgtcga caaccgtggc
     3901 atgtagcgta acttcgtcaa cttccttgcg gaagacaaag acatcgccgc tcttttcagc
     3961 tggggcttgt ccctgtgcgg cggacggagt tggttcgacc ggctttggtt gcggcggagc
     4021 cgtcgaagtc tgtccgacgg tgagagttcc caagaaaatt aaggcgacgg ctagaacgaa
     4081 aaagcttcta ctccgtgggt gcatagtatc ccgttttggc gtaaacgtgc aggggcggca
     4141 ggccctttgg cggattcaat ctcactttga tctttcgcca cttgccgtcc ctacttggat
     4201 tgctgggacg gtatccgaga acgtactggt tgcgcaattc aattcctatc ttggtggcta
     4261 cgtcggccag ttcgttggtg tttccgatgg tgaaggtgcg gccgcccgtg acatccgaga
     4321 cctcagccag cagttgggga ccttcgcgtt cttcgggagt cttgaaatca cggtcataga
     4381 ggccaatacc gtaaatctgc acgtccgcct ccttgaccat cgacttgatt tcggcttcgg
     4441 tgtagcggct atggttgtcg cctccgtcgg agatgatgag gagtgccttc ttctcgtgct
     4501 tggccttgcg catgcggttc atgcccagat agatggcgtc cagcagcgcg gtccgccctt
     4561 ttggagtggc atataccagc ttgctttgga tgtcttcgat ggaggtggtg aagtcggcga
     4621 gcacctctgg cttttcggaa aaggtgacaa ggaaaaactc atcttcgggg ttcgcggtct
     4681 tgaagaattc cacgaccgca tcgcgagatt tatcgatctt gttggccatc gaaccgctga
     4741 tatcgaagat cacgcccaac gatatgggag cgtcctcgct ggaaaagtgc ttgatctcct
     4801 gcggttggcc gctgtcgctg aggagaaaat tgtccttctc caggccggtc acaagccggt
     4861 tcatggggtc ggtaacggtc accggcacca gtaccaggtc aacgtctttg cggataggct
     4921 tggtgtgggt tttgagcgac ggatctaccg aaactccagc cgacttggcc tcggccgcaa
     4981 ttgccggtgg catgaccgtg gggtctggct tggcgtcctt tttttgctcg cgtgggacta
     5041 cgtgaatgtc agattgttcg ttctgggcaa atgcgggcac gacgctgcac gcgctgagga
     5101 ggagcacggc aaccattacg gcgaaactgt tacgcaggca caacaaagta tggacgggac
     5161 agaagctaaa acgtcccgcc gcatcgaagt caacccaggg ccgaatcatc gagctggctg
     5221 acccgactcc gttactgcca ccgcgggcca taggaacctc tacgcaagtg ggctgaatgg
     5281 tatcacaggt tgcaatcaac cttgtcatcg ttaccgctgc ccccaaagcc tgaattccgg
     5341 accttcaatg catattgatg ttgctgcaat cgcgcgggtt tcgttttctc aaactgctac
     5401 aatcgaaatt cgttccgagt gcagctttca ttcagaatgc gcgtcgggaa cccgattccc
     5461 atggcaaaaa tatacccttt taaagccctg cggtacgacc cagccaaggt gtctcccgct
     5521 caagtagtta cacagcctta cgacaagatt tctccagcca tgcaggaccg ttactatgcg
     5581 gccagtccct acaacctggt acgggtcatc ctgggaaagg ccgaaccggg tgacgacgag
     5641 cgaaacaatg tgtacacccg agccgctgcc aatttacaac actggcgggc ggagcatgtt
     5701 ctggcgcggg acgccgaacc cagcatttat ctctacacgc aaacctttaa tgtccctgga
     5761 gacccggcgg gaactcaact cgagcgccgc ggcttcattg ccgcagggca gcttgaggaa
     5821 tacgacaaga aagttgtctt ccggcacgag caaacgctga gcaagcccaa ggcggaccgt
     5881 ctcaaccttc ttaaggcaac gcaatcgcat tttggacaga ttttcatgct gtacagcgat
     5941 ccggcaggga aagtcgatgc gacgttgttg caggagcgca cacccgacgt tgaagtgcgc
     6001 gatgaatacg acgtcgtcca tcgcaagtgg aaggtttcgg acgctgcgac cattcagaag
     6061 gtccaggctt cgatggatga caaacagctc atcattgcgg atggccatca ccggtacgag
     6121 acggcgctga actatcgcaa tgaaatgcgg atgcgcggcg acggagatgc gaccgctccc
     6181 tatgagcgcc tgatgatgac cttcgtgaac atggattcac caggcctggt ggtattgccg
     6241 acgcaccggg tggtgtttgg actcgatagc tttgacgttg ccgccatgaa cgcgaagcta
     6301 ggcaagtatt tcgatgtcga agacctgggt gcgacgaccg acgtgaagcc cccaatggag
     6361 cggctgcggg atgctggcaa acagggttcc gcgttgctgg ccatcaccgg caaaggagct
     6421 tttctgctgc gttcgcaccc cgcgcaggac tcgcctgcgc tcgccggcca atccgagcgg
     6481 cagcgcggtc tcgacgtcgt gcaactacac aaactggtgc tggaagagac gctggggatg
     6541 tcggaggaag atatccgcga tcagaaacac ctaaagtatg tgcgcgaggc caaggaagcc
     6601 gccgaggaag tgggcaaagg cgcgaacgtc gccttcctga tgaatcccgt gcgcatgcag
     6661 caagttcggg atatcgcctt cggtggcgaa gtgctgccgc agaaatccac cgactttttc
     6721 cccaagctgc tcagcggcct caccatctat tcgttggagg acgacgccca aggcacggta
     6781 acaggaggtc actaggttgc ggctgtcgca cccgagggcg gtccccattg cggcgactct
     6841 gctcgcgatt gtgctgctgg gcggagccgt tcatttgctg cggctttcaa ccgtacaggc
     6901 gcagacgccc gcgactcagc ttgcggttta ctcggcgcaa acgtcttaca ccgtccccct
     6961 gctcaatctg aacggacagc catacgtcgg actcgtcgag ttgctggagc cactgggcac
     7021 cgtggatgct cgccctgacg gcaagaaata caagttgaaa ttcactccgc ccggcgcccg
     7081 cgagatggag ctccaatttc aggatggcaa agacaatggc aaggtgaagg gcgacaaaat
     7141 caagctgccg tcgatcttcg cgattcagaa tagccgggga tatgttccgc ttgcgtcggt
     7201 cagtgagcta ttgtcccgcg ctctgtctac ccagattcgg ctgaatccaa ccgctcggcg
     7261 gctgttcatt ggcaatatcg gcgaacgctt cacgcttgac ctgcgcaaag gcgagccgtc
     7321 gaaattgttt gtgagctttg actcggcggt aaatcccacg atcgcaaccg agccggggca
     7381 cattcgcttc actttccggc gtgaacccgt gatcgccgct gcggaccaca cggcctttac
     7441 cgacccgttg attacggggg cgaacttttc ggagcacgat ggcgtcgccg aactggatgt
     7501 gaccggcaac tcggccctga tggccaactt cgccgatggt ggcaagacca tcatcgtaac
     7561 cggcgcactc gcaccgcctc ctcctgctgt ggcccagttg ccgattcccc aagtgaacgt
     7621 cgcgccgcct gtcgctcagc caccgaaacc tcccgccgga ccgcgatttc tggtgctgat
     7681 cgacccagcc catgggggca ccgaaatcgg ttcagccatc agtcccacac ttcctgagaa
     7741 ggatgtggtg ctcgcactcg cgcgcaagtt gcagcgtgag ttgcaaagcc gcgggatcgc
     7801 cgcggggttg ttgcgaaacg ccgacatcgc catcacgctc gaccagcgtg ccatttcggc
     7861 caatgctgcg cggccggcca tttacgtcgc actccatgct gcaaacagcg gcaagggagt
     7921 gcatgttttc acttcgctgc tccctgccga gtcggtttcg tcgcgcgatt ttctgccatg
     7981 ggagacggcg caatccgcct atctcgatct cagcggatcg gtggcgggat ccgtgtccgc
     8041 cgaattagag gcgcgcaaac tacccaacgc cacgctggcg gcgcctctgc gtccgatgaa
     8101 caatatcgcg gcaccggcca tcgccatcga gattgcgtcg ccgagcaatg atgtcagcga
     8161 aatcgccagt ccggcctacc tcgaccaggt ggcgcaatcg attgccgccg gaattgccgc
     8221 cgtgcgcggc aaaatcccgg aggtgcgccc gtgagccggc cgatcgtcgt gatgttcatc
     8281 gtgctgctgc tgatcgcggc cggtctgggg ttctacgcct atcatctcaa ggccaaggtc
     8341 acggcggagg aacagcgcct ggctcaacag gcgcaagcag tcgtacccgc ggcgagcggt
     8401 ccgaccacgc tggttacgct ttacgttgcg tcggatgaag aaggcgggct gcgccgcacg
     8461 caacttaacg tcgcgcttcc gcaggaacgc agcgaaaggg cgcgagccat cttgcgcgcg
     8521 ctcctgggca gctatctgca gtctgggtcg cagcatccga ttggagctgg agccgacgtt
     8581 cgcgacgtct ttctgctgaa ccgcgatacc gccgtggtcg ataccaatgt cgcctttgcc
     8641 gatgcgcatc cctcgggcgt gctggtcgaa gaactcacca tcgcttcggt cgtctccacc
     8701 ctcaacgcca gcgatccgca gatcacccgg gtgaaaatcc tggtcgatgg caaagagcgc
     8761 gatacactcg ccggtcacgc cgacctgcgc cgattttatc tggccagcgg catcggccaa
     8821 gtgttgaagg acacgccgtg acccttcctc gtatcggagt attcgactca ggcgtcggcg
     8881 gccttaccgt gctgaaggcc atggccgagc aaattccggc gcactatttg tattttggag
     8941 acacggcccg tctgccctac ggaaccaagt ctgctgatac ggttgcgcgc tactccatcg
     9001 gtgcggcccg ttttctcgag gcccagaaca tcgatttgct ggtcattgcg tgcaacaccg
     9061 cgaccgctct tgctctgccg cagatcaagc aggccgcgcg ggtcccagtc atcggcgtag
     9121 ttgaacccgg agcggccatg gcagcggaag tcagccagag ccgctcggcc attgtcattg
     9181 ccacggaagc gacggtcagc agtcacgcgt atcgggacgc actgcgcaaa catggcatcg
     9241 aagcccatga gaaggcgtgc ccgttgttcg tcccgttggt cgaagaaggc tggattgaac
     9301 acccagttac cgagcaggtg gctcgaatct atcttggcga ggccatggcg caatcgtcgg
     9361 acaccgctga tgtgctggtt ctggcctgca cccactatcc cctgattgcc cctctgctgc
     9421 ggcgcattac gcctgaacac gtgaagatcg tggactccgc cgactcgacc gcgcgagtgg
     9481 tgcgccgcct gttgagcgag cgatcaaccc aaatcgaatc gccggagcgg gggaattcgc
     9541 gtaccgccgc tgcgtccaat caccgcagct tccgtttctt cgttaccgat tcggtggaga
     9601 agttcaagcg gctggccacc cgcttcatgg gacggcaagt tgacaacgtc gagcacgtgg
     9661 acctcggggg ctaaccccaa tagctgaaac ttcggagcga tacaatcgtt tcttatggtc
     9721 tatcgctccg acaatcgcac tcttgagcag atgcggccgc ttaatatcat ccccggctac
     9781 atctccaccg ctgaaggctc gtgcctgatc gagctcggca atactcgcgt gatttgcacc
     9841 gcgaccatcg aggacacggt cccgcagttt ctgcgcaata gcggaaaagg ctgggtcaca
     9901 gccgagtacg gaatgttgcc ccgcgctacg ttgacgcgca gcccgcgcga ggtgaccaag
     9961 ggccgggcca gcgggcgcac ccacgagatc cagcggctca tcggacgctc cctgcgggcc
    10021 gtcaccgaca tggacaagtt cggcgagcgc accatcttta tcgattgcga tgtcatccag
    10081 gccgacggcg gtacccgcac agcttccatc acaggagcgt tcgtcgccat gggactcgcg
    10141 atgacagcca tggttgagac cggcgcgctg agcgcggcgc cgattcgcga ctacgtggcc
    10201 gccaccagcg ttggcattgt ggatggcgaa atcatgctcg atctctgcta cgaagaagac
    10261 tcgcgcgccg acgtcgacat gaacctggtc gtgaccggca gtaagaagat cgtggaagtg
    10321 caggcgacgg cggagcatcg tccattcgat gacgcgcagt tggcccagat gatggcgctg
    10381 gctaagaaag gtatcgagtc actggtagca cgccagcagg ccgtgctgac agggctgccg
    10441 ctgcggcagt agcgggtgac ggcttgagga cgtagtcggt cactttccga gtcgcctatg
    10501 actcgggagg agcccggctg ctgagggtcg tcggaaccgc ataagccttc gcatcacttc
    10561 ctctacgcgt aagggtgctt ccatgtacaa ccagaaacga gccaacagac tcgcaggttg
    10621 cccagtgcga tccgggtgag ggcccgcgct gtcccttccg cgccaactga atttaggagc
    10681 aactggccga acgaaactac aaaatgatga atttattcgg cgacacccta gcatgaagtg
    10741 acaagctgtc cgaggaagcc tcgcccaact cgacccccga aaatccttgc ggctgttgcc
    10801 gtcgcgccgg agacagtggg gattcgcgca actttcatcg tctatttaca attccgtaac
    10861 tttccgaggc tacgattcgg tggaacgtgg attgaccggg cttccttttt tcacttcatt
    10921 ggagctacgt tcgtgcaatc gtttgcggtt catcgtgcca ctgaatttcc aggagagaag
    10981 atgaaaacta ctttggtgct tgctctggct gtagctctgt cccttttgtt gacggcgagc
    11041 gttttcgcgc agagctcctc ctcgtccgcc atgaaggaaa tgaaagacgc catcgctgcc
    11101 caacagcagc aaatacaaca actccaacag caaattcaaa gtcgggactc agccatccag
    11161 acgctggagc agcgggtaag ccaggcggaa gccgccgcca ctcaggcgca gtcaacggca
    11221 aaatcggcta tcgggggcga gaaaaaagaa ggcgctctga gtgccgaaga attcaatgcg
    11281 ctgcagcatg atgtcacgga cctgaaggca gtttcagcta acaccgtgga aggtctgcag
    11341 gatacgcaga aacgcgttgg cgatttggaa agcccgttgg ctatccactt caaggggatt
    11401 accctcaccc caggtggctt cctggcgggt gaaaccgtct ggcgtccaca cgccgccctc
    11461 ggcttcgcca cgccgttcag cgcaattccg ttttccgccg ccgcccaagc gcgctcctcg
    11521 gagttctacg gttcgggtag ccagtcgcgt ttatcgatgc tggcgcaggg aaagtggaat
    11581 accacaaatc tgacgggcta ctacgagtcc gacttcctgg gttctgctgt cacctccaat
    11641 gccaaccaga ccaacagtta cgtgctccgt cagcggcaac tgtggggcca ggcaggcttg
    11701 gccaacggct ggacgttcac cggcggccag atgtggagcc tggtgaccga aaccaagaag
    11761 ggtgaggaca accgcagcga agccacgccg atgcagattg acccgaatta caccgcgggc
    11821 ttcagctggg ctcgtcagtt cgcgttccgt gccaccaaga acgttaataa caaggtttgg
    11881 ttcggcgttt cggtggagaa cgcgcagacc atccttgcct cctcgggagc tgcggctaac
    11941 tacgtgatcg gcggcccggg cgtcggcgcg ggactatata accagtttgc caactactcg
    12001 accaacgaat cgcccgattt cgtcgccaag attgccttcg agccgggctt tggacactac
    12061 gaagtctttg gtttggccag cgatttccgc gaccgtatct atcccaacgc tacggccgcg
    12121 aagccgtcgg cagtgggagc gtataacagc agcatttggg gcggcggctg gggcgccaac
    12181 gcgcgctggc tggcctacca gaagcacatt gagctgggtc tgcacttcct gggtggcaag
    12241 ggcgtgggcc gctacggcga tacgcagttg gccgacgtga ccgtggaccc gaacggcaac
    12301 ctgaaggctt tgcaaaacta tcaggcgctg gtgagcttgg aatggcattc gccgaagtgg
    12361 gacttctacg gctacggcgg cggcgactac gtgggcaaga cgtggtactt gaactccgcg
    12421 ggcaaaccgg aaggctatgg ctcgccgctg tttaacaaca cgggttgcgg tactgagacg
    12481 gtgccgggaa catcgcagcc tttctatgca acaacagcag gctacgaacc cggagcctta
    12541 gcgagctgcg ttggcgagaa caagagcgtc attgagggca cgactgggtt ctggtataaa
    12601 ccctacaacg gtccgaaggg ccgcttgcaa atcggcgtgc agtactcgta tctggttcgg
    12661 actgcttggg taggttacgg cctgccgccg tacaacaacc tgccaggacc ggcgaccctc
    12721 gctccgaaag caatcgaaaa tatggtgttt acctcattcc ggtactacct gccttagtcg
    12781 cggcagccag tcgtgagaaa aaccgagggc ggccgagaac cgtcggctac ccttggtttt
    12841 tctgcgctcc gagagtttgg ttcgtccaaa tcgcgagcaa gaagcggcac gacgcgatgg
    12901 tggaaaagag gaagttgcct gagccgattg tcgtttgaat taaagaggga ttccaggaat
    12961 ccgaccactg atgtggacgt aaggccagca acccggaatt tgacccgcaa ccttccgcgc
    13021 tggtttctcg ctgtctgtcc ctgaattcat ttagaattga atgtcctgta catcttcaac
    13081 gcgggaccag ccatattttg taaggcggga ttcacatttc ataacaatcg ggcgcaccgc
    13141 gcctgaggag gatttgccgt gaaacttact cctggaaaac ttgcgggcat gaaggccgtc
    13201 tcgaatgaga acggcgtcat cgccgctgcc gcgatggatc agcgtggctc acttaagaaa
    13261 gcccttggaa cccgggccac cgacgaagga ctgatcgagt ttaagaatta tgtcactgaa
    13321 gttcttaccc agcacgcgag cgcgattctg ctcgatcctg agtggggcct aggaccgtcg
    13381 aagttgcgcg ctaaaggatc gggactgctg cttgcctacg agaagacggg ctacgacaag
    13441 gcgctttccg gccgtctgcc cgatctgctg gacgtttggt cggtccgccg cctgaaggaa
    13501 gccggcgcgg attgcgtcaa gattttgctg tactactcta ctttcgatga cgcgaagatc
    13561 aacgatcaca agcaggcatg gactgagcgt attggcgacg agtgccgtgc caatgacatt
    13621 ccattcttcc tcgagctgat cggctacaaa gagggcttgg aagacaaggg tcccgagtac
    13681 gccaagctga agccccaggt ggttgcggat tcgatggcgg agttttccac agaccgctac
    13741 ggcgtggatg tgctcaaggt ggaagttccg gtcaacctgc agtatgtgga aggtgcgaag
    13801 tcgttcaagg gccccaaggt ctacaccaag gccgaggcgc tgcagtactt ccgcaacgcc
    13861 gccaaggcga ccaacaagcc gttcatttac ttgtcggcgg gcgtgagcaa tcctgaattc
    13921 attgaagatc ttgagtttgc ccaggaagcc ggcgtgaagt tcaacggcgt gctttgcggg
    13981 cgcgccacct ggaaagatgg cattccaatc ttcgccgaca agggcgggaa ggccttcgaa
    14041 gcgtggctga aggatgaggg cgtgaagaac attgagaacg tgaacaacgc gctgaaggcc
    14101 gcgactccgt ggtacagcat ttacgaagtt cccgagcctg ctctggcgac caagtaattc
    14161 caggtagtct ttgacgctgt gacgtgactg aagcgcgtgg atgaactccg cgcgcttttt
    14221 ctttgggcct cagcctttca ctttctgcag tgggtacagt agccgccaat ctcgaagcgc
    14281 gagatcacga tgtgaaactg gcaatcgcgc tcgacctgtc cttttacgcg atcaaacagg
    14341 tcgctctcga actcctgcac cttgccacag cgcaggcagg tcatgtgcat gtgatcgcgc
    14401 tgcggacggc gctcgtagaa gtgtccctcc cccttcacgt gcagcaggtc aagttcgtcc
    14461 accaagccat gacgtttcag tagagatagc gtgcggtaca cagtgacacg attgatgtcg
    14521 ggatcgacct tcgaggcctt gcgcagtatc tgggccgcgt caaggtgctg cttggcggtc
    14581 tcgatgatct caaggatgat ccggcgctga cgcgtcagcc tgatcccgcg gcttaggagt
    14641 gcattttgca atgagcctgg cgcggccgat tccgggaacg gcatggttcg actctctcct
    14701 tgattgttgc atctgtttgc aacgatgttg caacataatc tattgcagcc gcttgaccag
    14761 cacctatctg ttaattcaaa tgtaagtaag taagtaagta agttgcaaca gtgttgcaac
    14821 tgaaattata gcactgaagt gattctccgc accaacattt tttgggacta accatgagca
    14881 atgcttttct tcttaggact ttcgcttcgc gctgcctcac cgtcataggc gtttcagcca
    14941 gcgagcgccc ctacttcatt acctacgacc agaccatgga agagccgggg acctggaaat
    15001 cgagatgaaa gggttgaccg cttccccgca ggatggcaac cagtttctgt caggggtaac
    15061 gaaattcgaa tatggcgcca agggttggtg gacgaccgaa ttctatcttg acgggcagac
    15121 taccgccaac cagagcacta tcttcactgg ctttcgttgg gaaaaccggt tccgcctgct
    15181 gatgcacgaa cacctcatca accccgtttt ttatgtcgca tgcgaggaca caaacggggc
    15241 cgacaagagc ctgatggaag tcgtcgggtt cgactcacag tatgacggtt tggtgccgaa
    15301 caaggtggcc agccgcgatc gccagggtga gttagaaacc aaattgatac tgtcaagcaa
    15361 tgtaaacggc tggaacattg ccgagaactt tatcgctgag aagaacctgg cgggagaacc
    15421 ttgggagttt gggtatgcgg tcgcggtgag ccgtccgcta tcgctcatgg cctctccgga
    15481 ggaatgtacc ctgtgccgcg aaaacttcca ggctggcgtt gagttctagg gcgggctcgg
    15541 cacggcgaac aagtttacgg tgcacggcac gtcgcagtac gtcgctcccg tactctcatg
    15601 gcaggtcggc aatacggtgt tcaaggtttc gccaacattt ggactgacgg atgccagtta
    15661 ccgggccatg ttgcgtttcg gagtgcaata cgaaatcagc ggccttcgac gcaagcttga
    15721 gaagctgttt cattgaggcc tgcctgtaaa ataccaacac aggagaaatc cttgaatcga
    15781 agcttgcgtc ttttgatcgt cagtaccgcg ttcctgacaa cggcagccat attctgcatg
    15841 gcgccccatc ttgccgcgca aacatttcac aacgcgcccg catcagccgc gcaaatgaag
    15901 aacccgtatg ccggtgacgc ccaggcagcg gcaggcggga aaaaactata cgcccagaat
    15961 tgcgcccagt gtcacggcaa taacttgcag ggcatgggtc cggctccgac cttgctgggt
    16021 ccgaaggtga aggatgccaa atccggcgag ctcttctggt ttattacgaa cggcaatttg
    16081 aataacggga tgccatcgtg gtcgcaattg ccgaagccgc aacgctggca aatcgtaacc
    16141 tttctggaat ccaggaactc cgctggccaa tagcactcta ctctgggtca ctgcaacgga
    16201 aagccaatat ccgctataac taaatagtga gtaccagcaa tcctttcttc gctcccaaga
    16261 cggccctgat cacgggcgca tccagcggca ttggcctcga actgtcgcac ctgttcgcgc
    16321 gcgacgggta ccgtctggtt ctggtggcgc gcaatcgcag cactttgcgc caactgggcg
    16381 acgatctgca agcgcgctat agcatcacgg tgcggatcga gcccaaagac ctggctcatc
    16441 ccgcaacccc ttcagagctt taccaggaat tgcaggagtc gggcatcgtg ctcgacgtgc
    16501 tggtgaacaa cgccggattt ggcggcgcgg gtgctttcgt cgataccgat tggaaccacg
    16561 aaggcgagat gatgcaggtc aacatcgtgg cgctcactca cctgaccaag ctgttcctgc
    16621 cgcaaattcg cgcccgcgaa ggcatgatca tgaatgtcgc cagcacagca gcatttcagc
    16681 ccggtccgtt catggcggtg tattacgcca gcaaggcgta cgtgctcagc ttcaccgagg
    16741 cgttagcgga agagctacgt ggaacaggcg tgaaagtttc gtgcctctgc ccggggccgg
    16801 tggagacgaa ttttcagaag cgcgcctacc ttgaaggcac cgctatggcc aatagtccct
    16861 taatggtcga cgtgcgggaa gtggcgcgca ttggctacga gggcttgaaa caaggtaagc
    16921 ggattgtcat cccgggttgg aagaaccggg tgggtgtcga attattgcgc atctctccgc
    16981 gagagatggt tactaaggtc gtcaggaaga tccaggaaaa gaagagcggc tgagccgcgc
    17041 ggcggccggg caaatccaaa tccgagcaag tcaactacaa gttgaacaac tcgcgaagcc
    17101 gcttggtttg ggcgcggctg accgggattt cggcctgctt cttgtcgtcc atgcggatct
    17161 ggtaggagct cttaaaccac gggacaactt ccttgatgcg attgatgttc accaggtagg
    17221 accggtgggc gcgccagaac acatttggat cgagggagtc cagcaattct tccagcgtgc
    17281 ggcaattgga ttgaccctcc atattggtcg cgactacggt gatgattccg tcctcgattg
    17341 aggcgtagca gatgtccttt tggtccacca gaaacaaacg cccgaccgac ttaagcagca
    17401 cttttgcagc ttgaggacgt tgttgctgtt ccagcatttt caccagggtg ccgatctggt
    17461 cgttggggga ggcggtcgat tcgagcttct tccgcgcctt ctcgaccgac tgggcgactc
    17521 gtttcttgtc aaacggcttc agcaaataat cgacggcgtt gacttcaaag gctttcaccg
    17581 cgtattggtc aaacgcggtg gcaaaaacga tttgaggcat cggaaccttg cggtcgagca
    17641 gcttcttgat caccccgaag ccgtcgagcc cgggcatctg cacgtcaagg aagacgaggt
    17701 cgggtgaaag atccttaatc aggttgacgg cctcaacgcc attttttccc tgtgcgacca
    17761 cgtcgacgcc aacctctttc aataggtagg caagttcgtc tcgcgccaat tgttcgtcat
    17821 cgacaatgac tgccgaaatc gacattcatg ctcctgaaag cgaattgaag aagaaagtat
    17881 aaacccctgt gggtcggtta gagcaggagc gataagtaat gggtcctcgc tcctgtcgta
    17941 cactggtatt cgatggtcgt tatcttcggg attcatgcgg tcagtgaggc gctgaagtcg
    18001 ggtgctcgca gcttcgatta cgtaggcatt gcgcgcgagc gccacgatca gcgggtgcag
    18061 cggttgatcg aagactgcca caccagcgga gtgcaggtgc ggttcctgcc tcgcgaagaa
    18121 ctcgaccgca cggccaacag caacacgcac cagggcgtga tcgcggtgac cagcagcaag
    18181 caatatgccg acgtcgatac gctgattgag cagcggcacg gcgagtatag cttcctggtc
    18241 gtgctggacg gtatcgagga tccgcacaac ctgggcgcgt tgatccggac ggccgacgga
    18301 gctggcgctg acggcgtcat cattcctgaa cgtcgcgctg tggggctgac ggggacggtg
    18361 gtgaaggcct ccgctggagc ttcggagcat cttgcgattg ctcgcgtcac caacatctcg
    18421 cgcaccctgg aagaactgaa agaccgcaac gtctggactg tcggcctcga tgagcgcggc
    18481 acgaaaagct acgacgaact tgattacaag atgcattgcg cgatcgtgct gggcgcggaa
    18541 ggcaaaggtc ttcacgatct ggtgcgcaag cggtgcgact tcctggtttc catcccaatg
    18601 ctggggcagg tagcttcgct gaatgtgtcg gtcgcgggcg cagtcgtgat gtacgaagtg
    18661 gcgcgtcaac gcagatcgcc gaagagtcag gggaaagcca agccctccaa atgaagcggt
    18721 ggctcacgct ctttgtgtgt tccctggcta tggcctgtac cgccgcggcc ctcgaccgcg
    18781 aagccttcac cgtcacccgc taccagttgg acgttcagat cgatcgcgct tcgcacgtga
    18841 tggctgtgac tggccagttg acgctgcgta acgattccaa gtcgcctcag aaatacgcga
    18901 cacttcaagt ctcgtcgtcg ctgagttgga acggcattgt ccttgaccgc acgccggtgg
    18961 aatggatcgg caataactac acttccgaca tcgatcacac gggcgggctg tcggaagcca
    19021 ttgtgaccct gcctaaagac gtgcctccgg gaggaaccgt caaccttgat gtgcagtacg
    19081 gcggaacagt taccccagac tccaccaggc tgacccgtat gggcgccccc gccgagatgg
    19141 cgctgcgcaa tgattgggac cagattagcg agccatttac tgcgatgcgg ggactgggtt
    19201 atgtggcttg gtatccggta tcgctgccag ccgtcagcat gagcgatgga aatgccgtgt
    19261 tcgacgccat cggtgcctgg cagttccgcc agcaacggac cgaattcgat gcgcgcatac
    19321 acgtcattag cggagacgcc aagctttgca ttgtgggaaa cgcgaccacc tcaagttgcg
    19381 gcgagatggg cgggacggag aatccgcgca ccggtggccg ggtgggacaa atcaccaata
    19441 gcattcacct caacgg
//
LOCUS       DASRKK010000089         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1080365, whole genome shotgun sequence.
ACCESSION   DASRKK010000089 DASRKK010000000
VERSION     DASRKK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080365"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFC15_01895"
     CDS             <1..601
                     /locus_tag="VFC15_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM08942.1"
                     /translation="IGHSTGGMLGIRYALMYPSQVDQLVLVDPIGLEDWKARGVPWQS
                     VDAWYQRELQTTAERIRTYERATYYAGTWEPGYDRWVQMLAGMYRGAGRAQVAWDSAL
                     LYDMIYTQPVVYELGALRMPVLLIIGDKDTTAIGKDLASSEVRATLGNYPVLGKVAAA
                     QIPHAKLVEFPDLGHAPQIQAPERFHKALLEGLQAPSTY"
     gene            1299..1967
                     /locus_tag="VFC15_01900"
     CDS             1299..1967
                     /locus_tag="VFC15_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZM08943.1"
                     /translation="MILIPFDEVRSDLRPRAAVVVAHPDDETLWCGGYILTHPEFRWR
                     VVTLCRASDPDRAPKFRQVLQQLGAEGEMADLDDEPAQVGVPIEQVQETIVQLLAGES
                     YSLILTHGPKGEYTRHRRHEECCQGVVDLWRSGGIDAGRLWLFAYEDGDRAYLPRVRE
                     DADRRQVLTDDIWQEKRRLIMDCYGYGADSWEARSTPREEGFRCFDSAQAAVIRTALL
                     EEKT"
     gene            1964..3106
                     /locus_tag="VFC15_01905"
     CDS             1964..3106
                     /locus_tag="VFC15_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM08944.1"
                     /translation="MKVLVLFDYPPPPGGLAMQGDLLCRGLREIGVDVHPVHLKSSLE
                     KEWYYRWFRPDVVVGVGYWGHVPDLVLHPQKFGMLAVPWLVADGFIAEYRDVLNALPL
                     ILVTSNWVKDVYVRDGIKGDNIEVLPVGCGTDRYVPHSSDDLKVKSIRKDLGVSEDQL
                     MILTVGGDGASKGAREVMEALALIDAEVPDWRYVCKVWPQPRTVQQNLLDLELAARLG
                     IDKKVIYSTSIVSQNFMPYLLAACDIYAAPSRLEGFGMIQVEANACEKPVIAIDAMAF
                     LDTMVHGETAFLAKVAEERKITEAVFGEGHGIESGQRIVFPNPRTAEYRASVPDIAKY
                     LLTLMNDSTLRREMGEAGRKHVVELFDYRQVAKRFVKIVSDRLGIQ"
     gene            3174..4358
                     /locus_tag="VFC15_01910"
     CDS             3174..4358
                     /locus_tag="VFC15_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HZM08945.1"
                     /translation="MDEKSIASIDQAKEAALRILLHNAHGQFQGLPRTAGWGYPEPYT
                     RDLMISALGFLVTGNEELADALRRTLVALAKNQTARGLIPSLAHDPDDRGASDTTPLF
                     LFGLALYRKSANLPEFLNQAAQSALKWMQYQSPDDRVMVSQLPTSDWRDEQWVMGYGL
                     YVNTLVYAYLKLYGEHESAGQLRELMNRLEVCGEAKNPHEHEGLVVPSKPYYALYSYK
                     VLNSDRFDLLGNSLAILTGIASPERARDLVAWIEAECEAMRARGELAVDLPSCLFPYI
                     LPHHADWHPRYEIYNRPGDYHNGGVWPFICGFYVAACVAVGRMDLANRKLLALTELVK
                     PWHENEAEWGFNEWIHAQTGQPSGRDWQTWSAAMYLYAVQCVQRQDTPFFGDMRPGDL
                     NR"
BASE COUNT         1123 a         1406 c         1631 g         1100 t
ORIGIN      
        1 gatcggccac tccaccggcg ggatgctggg gatacgctat gcgctgatgt acccgtcgca
       61 ggtcgatcag ctggttctgg tggaccctat cgggcttgag gactggaagg cgcgcggcgt
      121 accctggcaa agcgtggatg cctggtatca gagggagttg cagacaaccg cagaacgcat
      181 ccgcacttac gaacgtgcca cgtattatgc cggcacctgg gaaccaggtt atgaccgctg
      241 ggtgcaaatg ctagctggca tgtatcgcgg cgcaggacgc gcccaagttg cgtgggactc
      301 cgccctgctc tatgacatga tctatacgca gccggtggtc tacgaactcg gtgcgctgcg
      361 catgcccgtg ctgctgatca tcggcgacaa ggacaccacg gcaattggca aggatctggc
      421 atcctcggaa gtacgcgcca cgctcggcaa ttatcccgtg ctcggtaagg tagccgccgc
      481 gcagattccg cacgcgaaat tagtcgagtt tcccgatcta ggtcacgccc cgcagatcca
      541 ggcgccggag cgtttccaca aggcgctgct agaaggactg caagcgccca gcacgtacta
      601 gttcgacgac tctgaatgtg tgggccagag cagagtcccg tcgtcgcggg ccagaagtcc
      661 tgttctggga agtttcaacc gataggtaat ggaaaacgcc ccaggctccg ggagcatatt
      721 cgcccccccc gcccggtggc agggcacggg atgccagccg caaagccgac ttcctgttac
      781 aaatcggtgt gacctttgct ttgcggcggc agagttccaa gcacccgagg gatgttgcgc
      841 tgacggttga tagcatttgt ccatcagcga agggaacggg aaactaatcc agtcgattcc
      901 gccgcgttga aggttcgcga cacgatgctt tgagtcgcta gtcgcagacg gcattcgctc
      961 gacgagtttg tgcccggtgc aatccattga actgagcctt actgaccgga ctcgggcaac
     1021 tccggagtga gaaggttgaa gccagaactg tgtttcagcg gagcgaatcc ttcagtacga
     1081 aggccgagtg cgctaatgaa ggacgccagt aaaccatcgt ctggcagcta attcagcttc
     1141 gcgaaccaag gagtgatcgc aatggcgagc gcaacagccg tgaaggaccc aggatgcggc
     1201 atggacatgg atacggccac cgctgctgga cacactgagc atgccggttt ggtgcgcgaa
     1261 tacttgcagc catgccctga cctctgccga agaagccaat gatacttatt ccgtttgatg
     1321 aggtgcgctc agaccttcgg ccacgggccg cagttgtcgt cgcccatccg gacgacgaga
     1381 cactctggtg cggcggctac atactcacgc atccggagtt tcgttggcgt gtcgtgacct
     1441 tgtgcagagc atcggatccc gatcgtgcgc caaagttccg ccaagtcctg cagcaattgg
     1501 gggcagaggg tgagatggcg gatctggatg atgaaccggc ccaggtgggg gtaccaatcg
     1561 agcaggtgca ggaaaccatc gtacagctat tggctgggga gagctacagc ctgattttga
     1621 cccatggccc gaagggcgaa tacacacgac accgccgaca tgaggagtgc tgccaaggcg
     1681 tcgttgacct ttggcgatcg ggcggtatcg atgcagggcg attgtggttg tttgcctacg
     1741 aagatggtga tcgggcgtac ttgcctcggg ttcgcgaaga cgcagatcgg cggcaggtgt
     1801 tgacagacga tatctggcaa gaaaagcgca gattgattat ggattgttac gggtacggtg
     1861 cggacagttg ggaagcgcgg agcacacctc gagaagaagg tttccggtgc tttgactcgg
     1921 cacaagcagc ggtaatacgc acagcattgc tggaggaaaa aacgtgaagg tgcttgtgtt
     1981 gttcgactat ccaccacctc cgggcggcct ggcgatgcag ggcgatcttt tgtgcagggg
     2041 tctgcgcgaa atcggcgtgg acgttcaccc ggtacatctc aaatcgtcac tggaaaagga
     2101 atggtactac cgctggtttc ggccggacgt tgtggtgggc gtggggtatt ggggccatgt
     2161 tccggacttg gtcttgcacc cgcagaagtt tggaatgctg gcggtcccat ggttggtggc
     2221 ggatggtttc atcgcggaat atcgggacgt tttgaacgct ttgccgctga tcctggtcac
     2281 ttccaactgg gtgaaggatg tctacgttag ggacggcatc aagggagaca acattgaggt
     2341 gcttcctgtg ggttgcggca cggaccgcta tgtgccccat agcagtgatg acttgaaagt
     2401 caaatcgatc cgcaaggacc tgggagtatc ggaggaccag ctgatgattc tgaccgtcgg
     2461 cggcgatggg gcgtccaagg gtgcccggga ggtgatggag gcgctggcgc tgatcgatgc
     2521 cgaggtaccc gactggagat atgtctgcaa ggtttggcct cagccccgca ctgtccaaca
     2581 gaacttgctc gatctcgagc tggcggcgcg tctgggcatc gacaagaagg ttatctactc
     2641 gaccagcatc gtgtcgcaga atttcatgcc ctacttgctg gccgcctgcg atatctacgc
     2701 tgcgccgtcg cggcttgagg gtttcgggat gattcaggtg gaggccaacg catgcgaaaa
     2761 gccggtgatc gcgatcgacg cgatggcgtt tctcgacacg atggtccacg gtgaaacggc
     2821 gtttctagcg aaagtagcag aggagagaaa aatcactgag gcggtattcg gggaaggcca
     2881 cgggatcgag agtggccaac gtatcgtgtt tccaaacccg cggacagcgg agtaccgagc
     2941 cagcgtgccc gacatcgcca aatacctcct gacactcatg aacgacagca ctctgcggcg
     3001 ggagatgggt gaagcgggcc gaaagcatgt cgtcgagttg tttgattatc gccaggttgc
     3061 caagcgcttc gtgaagatcg tctccgaccg attggggatt cagtagagag taaggtggtc
     3121 aggagcgagt gctcctggtc gcagctttgc atgccataga ggtggcaatg tttatggacg
     3181 aaaaatcgat cgcctccatt gatcaggcca aagaggcggc cctccgcatc ctgctgcata
     3241 acgcgcatgg gcagtttcaa ggacttcccc ggaccgcagg ctggggatac ccggaaccgt
     3301 atacgcggga cctgatgatt tcagccctgg gttttctggt caccggcaac gaagagcttg
     3361 cagacgcgct gcggcgcaca ttggtagcgc tggcgaaaaa tcagacggcg cgtggcctca
     3421 ttccatcact cgcccacgat ccagatgacc gtggcgccag cgatacgacg cctctgttcc
     3481 tctttggcct ggcactttac aggaaatcgg ccaacctgcc ggagttcctc aaccaagcag
     3541 cgcagagcgc cttgaagtgg atgcaatacc agagccctga tgaccgggta atggtctccc
     3601 agctaccgac cagcgactgg cgtgacgagc agtgggtgat gggttatggg ctgtacgtga
     3661 acacactggt ttatgcttac ctcaagcttt atggcgagca cgagtccgcc ggtcaactgc
     3721 gcgagttgat gaaccgtctg gaagtctgcg gcgaggccaa gaacccccat gagcacgagg
     3781 gtctcgtagt gccatccaag ccttattacg cgctctactc ctacaaagtc ctgaacagcg
     3841 accgtttcga tctgctgggc aacagcctgg caattctgac tgggattgct tcgcctgaac
     3901 gggcgcgaga tctagtggca tggattgagg ccgagtgcga ggccatgcgg gctcgcggag
     3961 agctggccgt ggatttgccg tcctgtttgt ttccttatat cctgccccat catgccgatt
     4021 ggcacccgcg atacgagata tataatcggc ccggcgacta ccacaacggc ggcgtctggc
     4081 cgtttatctg cggcttctat gtggcggcat gcgtggcggt aggtcggatg gatctggcga
     4141 atcggaagct gttggcgcta accgaactgg tcaaaccgtg gcacgaaaat gaggcggagt
     4201 ggggtttcaa cgagtggatt cacgcgcaga ccggccaacc gagtgggcgt gattggcaga
     4261 cctggtcggc cgctatgtac ctttacgctg ttcagtgcgt gcagcggcag gacactccgt
     4321 tcttcggcga tatgcgccca ggcgatctga atcgttgagg acatcgaaaa ctttcacttg
     4381 aggatcgagc aggatatttt acgaatcaaa ttgcgggcgt gcgaggcggt gcctgccgag
     4441 gcccaggaga tggcccacgt cggcaactgg gacatcgacc tggttgccaa gagctacctc
     4501 tgctcagcgg aagcatatcg gactttcggc gtcgattcgg caatgttcca gccctcgttc
     4561 aactcgatac aggctcgaat tcaccctgat gaccaacggg cggtcggaga agatatcgcc
     4621 gaaactgtct cagccgtgtc ggagactaaa gggagtgggg cctgtcgcaa gacgaagcaa
     4681 gggtacaaac aaaggtacat ttgtagggcc gatatgctct ttagaagact tcaggctcct
     4741 tgccgtactt gaaaatagac tgtaaaccta tgattattat agtacttgaa ctctgtcacg
     4801 gcagaggttg gtcggattac tcctggaaaa cgccccagtg tccgggaatc cacttggacg
     4861 gttagtcggc aaaccgttga actgccactc gcgtgcaaac tcccgcgata ccggcatggg
     4921 ggagatgccg aaccggcgta ccagcggcat tgttggtggc tcacctttcc gcgccatcgg
     4981 cgggtagtaa tcgatctgag cgtgcgactt gcatccttta tatagacatc gtcctgcttt
     5041 gcgatcttct ttctgctcta ccccactctc aaccctagta ctgccaacaa aacaagacac
     5101 ttagcgcact tgctggctgc tgcgtaagcc gaggtgtgcg ccatggtctc gtcttggctt
     5161 tcaacacaac acgaattggg cggactgccc ggaaaggatc accgcaatgt ttgggcaaat
     5221 gatggttgaa cccgttgtcc agttcgaccc cggcctcgat
//
LOCUS       DASRKK010000090         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_396341, whole genome shotgun sequence.
ACCESSION   DASRKK010000090 DASRKK010000000
VERSION     DASRKK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396341"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFC15_01915"
     CDS             <1..690
                     /locus_tag="VFC15_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM08946.1"
                     /translation="GYSGLTVGTAGSSDGTVTKIDAQSPAASAGFQVGDTITAVNGNA
                     AELTPGMIAAKSVFGQRGDTLNLTLKRAGSPVNVSLVRGPQTAPKGPTASGFMTEVKL
                     MINWQNQFAPCIGIGPAAPVAIGFCYGHFKPYGFIKAEELGSTGFEIDLANASKAPIT
                     AVAPDSPAAKAGVQPGDEIVAVGGQPLTASRGEEASELLFGKSGDRLQVKVQRGEANP
                     TLELVLASKGK"
     gene            complement(1037..2230)
                     /locus_tag="VFC15_01920"
     CDS             complement(1037..2230)
                     /locus_tag="VFC15_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM08947.1"
                     /translation="MEKRLSGWKRELLRSHDRIAGLFARSEARERSLAYLQGLMSHCE
                     RKNGWQLAEWMGEAAPYRVQHLLDRARWDADAARDELRNYVLEELSSPDAVLIADETG
                     FLKKGEHSVGVKRQYTGTAGRIENSQVGVFLCYASDKGAALVDRELYLPEEWAADSER
                     RRAAAVPEETEFATKPELARRMIERALKAGALCSWVAADEVYGNNSKLRQWLEERRLG
                     YVLAIASDQRMRWPDHQQRRVDTIAQSLPNQAWERVSAGSGSKGERLYDWALIQGWQE
                     DGWSHGLLVRRSIEEEPEHAYYWFYAPTCKAKLEVLVRVAGQRWQIEQAFQAAKGECG
                     LDHYEVRHWQGWYRHITLAMLAHAVLAILRARGEKNSRRAGAAQRTRTAPPTHSPSLA
                     RMARR"
BASE COUNT          530 a          801 c          723 g          524 t
ORIGIN      
        1 ggctactcgg gtctcaccgt cggtacggca ggcagcagcg acggaaccgt tacaaagatc
       61 gatgctcagt cgccggctgc cagtgcgggt ttccaggtgg gcgacacgat cacagcggtg
      121 aatggcaacg cggccgagct cactcccggg atgatcgcgg ccaaatcggt cttcggacag
      181 cggggcgata ccttgaacct gacgttgaag cgcgccggct ccccagtgaa cgtaagcctg
      241 gtacgtggtc cgcagactgc acccaaaggc cccacagctt caggcttcat gaccgaggtc
      301 aaactgatga tcaactggca gaaccagttc gctccctgca tcggcatcgg gccggcggcg
      361 ccggttgcca tcggtttttg ttatgggcat ttcaagccgt acggattcat caaggcagaa
      421 gagctgggct cgacaggttt tgagattgac cttgccaatg cgagcaaggc cccgatcaca
      481 gcagtggcgc cagactctcc cgcggcgaag gccggcgtgc agccgggtga cgaaattgtg
      541 gccgttgggg gccagccatt gactgccagc agaggagaag aggcttctga gcttctcttc
      601 ggaaagtcgg gcgaccggct gcaagtcaag gttcagcggg gcgaggcaaa tccgactctc
      661 gaactcgttt tggcgtccaa gggaaaataa cttccggcgg cctcatgaac atggaagaat
      721 tctttgcgat atgaagcgcc ctggtagcgc atcatcgatt ccccacgcca gttcgtagag
      781 acgttgcccg ggatactcat cggacatagc tcctattcgg gctactgctg tgaacgaagt
      841 gcttgttgcc gacaatcgtg gcatccgtgc gggcttcgga aaggcccgaa tcgatgattt
      901 ttatcgccgg gtgcccagga cctagtacta cagttgtaga tagaagatcg gcagcggcga
      961 gcctcgtttc cgatagtgac agcacagggc gtggaattga tgatgccttc gccattggga
     1021 ccagtggaga agatgctcaa cgccgtgcca tccgcgccag agaaggtgag tgagtaggtg
     1081 gcgcagttcg ggtacgctga gcggcacctg cccgtctgga gtttttttct ccccgtgcgc
     1141 gtaggatggc cagcaccgcg tgggccagca tcgccagagt gatgtgccgg taccagcctt
     1201 gccagtggcg gacttcatag tgatccagcc cgcactcgcc cttggcggcc tggaaggctt
     1261 gctcgatctg ccagcgttgt ccagccacgc gcacaagtac ttccagcttg gctttgcacg
     1321 tgggcgcgta aaaccaatag taggcatgct caggctcttc ttcgatgctg cgccgcacca
     1381 gcaatccgtg gctccaacca tcctcctgcc aaccctggat tagagcccag tcgtacagcc
     1441 gctcgccttt gctgcctgag ccggctgaaa cgcgctccca ggcctgattc ggcaaactct
     1501 gtgcaatcgt atccacgcgt cgctgctggt gatcgggcca gcgcatacgc tggtctgaag
     1561 ctatggccag cacataaccg agccgccgtt cttccagcca ctggcgcagt ttgctgttat
     1621 tgccgtagac ctcatcggca gctacccagc tacagagtgc accagccttc aacgctcgct
     1681 cgatcatgcg tcgcgccaac tctggcttgg tggcaaactc cgtctcttcc ggcactgccg
     1741 cagcgcggcg acgctcgcta tcggcggccc attcttcggg aagatacaac tcacgatcca
     1801 ccaacgccgc acccttgtca ctggcgtagc agagaaacac gccaacctgg ctgttctcga
     1861 tgcgtcccgc cgtgccggtg tattggcgct tgaccccaac cgaatgctcg cccttcttca
     1921 gaaaacccgt ctcatcggcg atcagcacag catccgggct gctcagttct tccagtacgt
     1981 aattgcgaag ctcatcccgc gctgcgtctg catcccaacg cgcccgatcc aacaaatgct
     2041 gcacccggta aggagccgct tcgcccatcc actcagccaa ctgccagccg ttcttacgct
     2101 cacagtggct catcaaacct tgaagatagg caagactgcg ctcccgcgcc tccgaacgcg
     2161 caaacaaacc cgctatgcgg tcatgggacc gcaacaattc acgcttccaa cccgatagtc
     2221 gcttctccat agctctcgcc ggagagtcgt atcccagagc aagctcagat tatcattaac
     2281 tacaactgta gtactagaac gctgtgaagt tttggggatt gattaatgat tttgcaataa
     2341 gcgccttaga actcggtccg ggttgcgagt gaaaatcgcg caagcgtata gtaggcccgt
     2401 gccagacacc aagaaagtcg ttccgccaaa accagctccc ggatcgtgcc catgatcagc
     2461 cgacgaagct ttcttgccgc actttcagcc tctttgactt cgctgccggc cgcgttggcg
     2521 caagccggtt accgcaagcc tgccaaccgc aacgtccagt gggcgctctc tgaggcgc
//
LOCUS       DASRKK010000091         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_103088, whole genome shotgun sequence.
ACCESSION   DASRKK010000091 DASRKK010000000
VERSION     DASRKK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103088"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFC15_01925"
     CDS             <1..318
                     /locus_tag="VFC15_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08948.1"
                     /translation="ILCERGIRTFETYTRNTLDISAIPVVHKLSHLPITSDPSHGTGL
                     RDKVAPMACASVAAGADALLIEVHPSPDEALSDGAQSLYPQQFSELMDQLRMIAPAVG
                     RTI"
     gene            568..1425
                     /locus_tag="VFC15_01930"
     CDS             568..1425
                     /locus_tag="VFC15_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HZM08949.1"
                     /translation="MAIRQITIVGTGLIGGSFGLALRQQGFRGALVGCDRPEVLDAAV
                     IRGAIDRGDTDVRQAVEGCDVVVLAIPIGGILSTFEKLVPTLSPDTLVTDTGSTKVKF
                     VERARMIFGAEAGIRVLPGHPMAGKERGGIENADADLFRDAVWLITPISGEQQYTERQ
                     REYIELLESIGTRVVAMDPERHDRLCAWISHLPQMIATALASVLREELGDDEAIREIG
                     GRALREMTRIASSPYSMWRDIALTNSQNIDEALLRFEQQLAHLRENLRGPGLREMFES
                     ANKFGKDRG"
     gene            complement(1995..2261)
                     /locus_tag="VFC15_01935"
     CDS             complement(1995..2261)
                     /locus_tag="VFC15_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08950.1"
                     /translation="MTLIHHIEHIEKKINELHKSLIALGSQDPVPALIPIIHKPGWTS
                     VAEEAFVTGILDAMLSQTKTLSELKQILLSGVNKVALNPQPLPP"
     gene            3653..4354
                     /locus_tag="VFC15_01940"
     CDS             3653..4354
                     /locus_tag="VFC15_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZM08951.1"
                     /translation="MAMNKVVANADEAIHDIKDGDTLMVGGFGLCGIPENLIRALVKK
                     GVKDLNTISNNVGIDDFGLGLMLAAGQIKSHKGSYVGENRLLEEMVLGGRIDLELIPQ
                     GTFAERIRAGGAGIPAFYTPAGFGTVVAEGKETREFDGRPYVLERALKADFALIKAWK
                     GDRWGNLVYRKTAQNFNPMMATAAKVTIVEVEHLVDPGEIEPDHVHTPSVYVQRIFQG
                     TDYEKRIERRTLRKT"
     gene            4384..>4769
                     /locus_tag="VFC15_01945"
     CDS             4384..>4769
                     /locus_tag="VFC15_01945"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZM08952.1"
                     /translation="MPNEIAVKQKATEKRDRIVKRVARELRDGFYVNLGIGMPTLVAN
                     CVPPGMDVVLQSENGMLGVGPYPLAGEEDGDLINAGKETVTELPGTAFFSSAESFAMI
                     RGGHIDLSVLGAMEVDEEGNLANWMIP"
BASE COUNT         1001 a         1304 c         1423 g         1041 t
ORIGIN      
        1 attctgtgcg aacgcggcat tcgcacgttt gagacctaca cgcgcaatac actcgatatt
       61 tccgccattc ctgtcgtgca caagttatcg cacctgccga tcacctccga tccttcgcat
      121 ggaaccggat tgcgtgacaa ggtcgcgcct atggcgtgcg cctcggttgc cgccggggcc
      181 gatgctttgc tgattgaagt gcatccgtcg cccgacgagg cgctgtccga cggcgctcag
      241 tccctgtatc cgcagcagtt ttccgaactg atggaccagt tgcggatgat cgctcccgca
      301 gtgggccgga cgatttgagc tgagctttaa ggtcttgaag gggcacacct ttaggtgcgc
      361 cgttaagttt tttattattg tcatcacgag cgggcttcag cctggcttca gcccgcgagg
      421 gatctgctgt tgacgggact tcccacgcac aaaaggaaag cagatgcctc gcttcgctcg
      481 tcatgacaac tcgatgggct agtttgaagg ggcacggctt tgaagccgtg ctccacccgc
      541 gctatacagg ggtgcgatgc tctagccatg gcgattcggc agatcacgat cgtaggaacg
      601 ggcttgattg gtggctcttt cggacttgcg cttcgccagc agggtttccg gggggccctg
      661 gttggctgcg accggcccga ggtgctggac gctgctgtca tccgcggcgc gattgatcgc
      721 ggagacactg acgtacggca agcggttgag ggctgcgatg tcgtggttct cgccataccg
      781 attggcggca tactttccac cttcgaaaag ctggtcccca cgctttcccc cgacaccctg
      841 gttaccgata ccggcagtac caaggtgaag ttcgtcgaac gtgcgcgcat gatattcggc
      901 gcggaggcgg gaatccgcgt gcttcccgga catcccatgg ccggcaagga gcgcggcggg
      961 atcgagaacg cggatgccga tctgtttcgc gatgccgtgt ggctgatcac gccgatcagc
     1021 ggcgaacagc agtacacgga acggcagcgc gagtacattg aactgctgga gtcaataggg
     1081 acacgtgtgg ttgcgatgga cccggaacgg cacgatcgcc tgtgcgcgtg gatcagccac
     1141 ctgccgcaga tgatcgccac agcacttgct tctgtactgc gcgaagaact gggagacgac
     1201 gaagcgatta gggaaatcgg gggccgggct ttacgggaga tgacacggat tgcaagcagc
     1261 ccctactcga tgtggcgcga catcgccctt accaacagcc agaacatcga cgaagccctg
     1321 ctgcgcttcg agcagcaact ggcgcatctg cgggagaacc tgcgcgggcc ggggctgcgg
     1381 gagatgttcg agagcgccaa caagttcggg aaagacaggg gctagtggct agtggctcgt
     1441 ggttgggggc gagtgactgt tagcgtccac cgcgtgtctt cgatgttact ttgtgcccca
     1501 ccaccactta caaaccctgt tcgagatagc ttacgataag ggctgttatc gctatctatc
     1561 gttgccctgg gggcaacctg agccgcgagg ctggtggcta atgccccgcg aggaacttcc
     1621 tccttttctg cttgatggca ttcagcctct tggcttgaac agtttcagcg acacactcct
     1681 tcgcgccttg acctgccgct acaatagcgg cttgatgttc cacaaactgt ggcctcggct
     1741 tcggtgaggc tgacggagtt tgtcggcttc attgcgactg tggttcgaag ccttcaagga
     1801 gtgggctagg agccggcgtc cgggctaaac gcgcggcgcc ggttgcggat gaaactcgac
     1861 aactaatgct gcccgtacct tacggaacta ccctcccaag cgaaaaaaac ttgggagggg
     1921 cacccgtctg cttattactc accacaaacc ctaatcgcca ggtgctagtg cgcctcggtc
     1981 agtgttggcg agcctcatgg tggcaagggt tgcggattca gcgcgacctt gttgacgccg
     2041 ctcaacagga tctgcttcag ttctgacagg gttttggttt gcgaaagcat cgcgtccaaa
     2101 attcctgtaa cgaacgcttc ctctgccacg ctggtccatc ccggcttatg gatgatagga
     2161 attagcgcag gcacgggatc ttgactgcct aacgcgatca ggctcttgtg cagttcattg
     2221 atcttcttct cgatatgctc aatgtggtgt atgagagtca agagttattt cacttaacac
     2281 agtttcttgc cgagccccca aacttctttt gcttaagtag gtgaagggac tggggaggtc
     2341 ctgcctgcaa cgcctcgggt agctgttgaa atatccccat tacacaagtg atccggctcc
     2401 gggcgtcgct ccgcaggatt gcttgtgtaa tgggctgatt tcgtcagttg gctcgtggcg
     2461 acgtggtaag ctgcggatgc aatggctcta ctgctgtctg ccgttatact cgccgcaacc
     2521 gcgtgtgtct tatggttttt acctcgaaac agccagcggc tctggaaact cactataagg
     2581 attcccgctg gcattttgat gtgcgcctca gcactgacgc tactgggctt tctattccgc
     2641 ggtgccatgt gtggccgcta tgagtttcct cctatattgt cgcgtgacgg aagactagtt
     2701 gctcaactga gcgaagtaga ttgtggcgct gtggactcgt ttcacagttc tgtgcagctc
     2761 tggcgaaacc gtcgaggttc tttcatgcac ctattcggga agcaagggca ctcgacgacc
     2821 atatttacag tgggacatga ccctcggttg attgtcgtcg tgtggaaaga cgaccgaaca
     2881 ctgctcattc gttacccaaa cgactcccgc tatgctgagg aatttcgctg tcaaccagaa
     2941 tgggaaggta ttcatattga gtgcgtggga tacgcgccgg attacactaa acccgtaggc
     3001 caaatgccac ctgtccgaag gtggctctgg tgaaggctgt aagctcgttg gcgtaatatc
     3061 cccatttcac aagtgatccg ctgccgggga tcgtttacga taacgcctgt tttggtagct
     3121 tatgaagcga ctcttagtaa ttcttctcat ggcggtcccc gcattcgccg cacctcagaa
     3181 gtgcaaggga aacccgaagg tcgtcagtgc gtgctactcg gttcacggta ggctccaatt
     3241 gggagccaac acagtgcgcc tgtacctttg gccagtggga acgaaaagga tgctaggcgt
     3301 cacggcaggg ccgcgacttg acgacgcaga cgatccaatc tggccacgca acttagacct
     3361 tagccaagat gtcgaagcca tttatgctga tttcgaggtg tgccccttca cgcccgagcg
     3421 gaaaggcgag atgcaattgg tgtgcattga gtcggcttcc catgttgtcg taaagcgcta
     3481 tcctccctcg aagcagtagg gtaactggcg taacatcgcc attacacaag taaaagacaa
     3541 aaccgcgcaa agcatttgga tcgagccgga ttgcgtggaa ctcgtggacg attgaccttt
     3601 ttgcgtcgag gcgacttgac tcggtacaat cgccggtcgg aatcaaaatc ctatggcaat
     3661 gaacaaagta gttgcgaacg cagacgaggc cattcacgac atcaaagatg gcgacacgct
     3721 catggtcggg ggcttcggcc tgtgcgggat ccctgagaac ctcatccgcg ccctggtcaa
     3781 aaaaggcgtg aaggacctga acaccatcag caacaacgtg ggcattgatg actttggact
     3841 cgggctgatg ctcgccgctg gccagatcaa gtcgcacaag ggaagctacg tcggcgaaaa
     3901 ccgcctgctg gaagagatgg tgttgggcgg gcgcatcgat ctcgagctca ttccgcaggg
     3961 caccttcgcc gaacgcatcc gcgctggcgg agcgggaatc cccgctttct acacccctgc
     4021 gggcttcggg acggtggttg ccgaaggcaa agagacgcgc gagttcgatg gccggccgta
     4081 tgtactggag cgggccctga aagcggactt cgcgctcatc aaagcctgga agggcgaccg
     4141 ttggggcaat ctggtttacc gcaagacggc gcagaacttc aatcccatga tggcaactgc
     4201 ggcaaaggtg accatcgtcg aagtcgagca cctggtcgat cctggagaaa tcgagccgga
     4261 ccatgtgcac acccccagcg tttacgtgca gcgcatcttc cagggaacgg actacgagaa
     4321 acgtatcgag cggcgaacgt tacgcaaaac ataattttgt gcaaggcgaa ccaggagaaa
     4381 cccatgccca acgaaatcgc tgttaagcag aaggccaccg aaaagcggga tcgcatcgtg
     4441 aagcgggtgg cccgcgagtt gcgcgatggc ttctacgtca atctcggaat cggaatgccc
     4501 accctggtgg cgaactgcgt gccacccggc atggatgttg tgttgcaaag cgagaacggc
     4561 atgctgggcg tgggaccgta tcctctggcg ggcgaggaag atggggacct gatcaacgcg
     4621 ggtaaggaaa cggtgacgga acttccgggc acggcatttt tctccagcgc cgaatcgttt
     4681 gccatgatcc gcggcggcca cattgacctg agcgtgctcg gcgccatgga agtggatgaa
     4741 gagggcaacc ttgccaactg gatgatccc
//
LOCUS       DASRKK010000092         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1080759, whole genome shotgun sequence.
ACCESSION   DASRKK010000092 DASRKK010000000
VERSION     DASRKK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080759"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFC15_01950"
     CDS             <1..322
                     /locus_tag="VFC15_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08953.1"
                     /translation="RGRFDDVQKIVPTAMAQPSRSAENYFDAANELYLSGRDFPDAAQ
                     YLQKYLDSGGLVENAPPFRAHYLLGQIYEKMGRSSDATSEYEASLALASGFDRARKAL
                     GRLQ"
     gene            512..1711
                     /locus_tag="VFC15_01955"
     CDS             512..1711
                     /locus_tag="VFC15_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM08954.1"
                     /translation="MALQHSGVMGIATVNQWRAREAYEEVRANYIPQVTIGSGLGYSY
                     GFPLTLEGSAPSVVNFNSAQSLFNLSLRQFLKAAKIDWNASSLDVQDKRDSVILDTAL
                     TYAQLDQLTGKLKALGEAQSAADNAQFVTEQRLQQGVDSKLDLTKSQLVAARIRLRIA
                     EAQGQADVLREHLSKLLGLPAESIAVEPASMPELPVISQDDNLPARAVENSLAVKQAE
                     ERVAAAQARATGEHKALKMPIIDLASQYAYLAKYNNYDLYYRNYTANNFSGGLNIRFP
                     IFNSAQKAKAEQADASAMIAKKQVEITKNQVSEDALKLQRSLRQLAAARDVSRLEWEV
                     AQGDLEAVKARIETGNGNTRDQQNARLDAEDKHAAYFDAQLEVSRAELQLLRLTGELE
                     KWAIPPR"
     gene            1826..3358
                     /gene="hpnJ"
                     /locus_tag="VFC15_01960"
     CDS             1826..3358
                     /gene="hpnJ"
                     /locus_tag="VFC15_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HZM08955.1"
                     /translation="MPLKTLFLNPPSFENYDGGASSRWPATREIESYWYPVWLAYPAG
                     MLEGSRLLDASPHKVTAQETIQIGRDYEFLVLFTSTPGFQGDIKLAAAMKAANPGLKI
                     AFVGPHVTTLPEPSLKDGQAIDFIVRREFDYAVVEYANGKPLNEILGASYRGPNGQIL
                     HNPDRPAVADLDALPDVVDVYRRDLDPRRYSVPFLLYPFVSLYTTRGCPAQCTFCLWP
                     QTLSGHPWRKRSTDAVAAEMAKAKEYWPWVKEFFFDDDTFNIQKARTIELCTKLKPLK
                     LTWSCTSRVTTDYETLKAMKESGCRLLIVGYESGDQQILKNIKKGATVERARDFTRDC
                     KKLGLIVHGDFILGLPGETKETIRRTIDFAKELDVDTIQVSLAHAYPGTELYDFAKAN
                     GFLKAEGSQMVDAMGHQVAMIEYPGISRDYILEMVHRFYDEYYFRPKAVFRIVKKAVF
                     NSSERKRLYKDAKSFLKLRAARNRSVKESRNQPGAINPPAPPVGTSKAGAKSETVEPV
                     SA"
     gene            3367..>3590
                     /locus_tag="VFC15_01965"
     CDS             3367..>3590
                     /locus_tag="VFC15_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08956.1"
                     /translation="MTFRKYLVLFAVVLFGACGDVCLARGMKDFGAVTSANWRHLFAS
                     LWNPWVLSGICLLLLFLCSYLSALSWADLT"
BASE COUNT          774 a         1042 c         1027 g          747 t
ORIGIN      
        1 gcgtgggcgc ttcgacgatg tgcagaagat cgtgccgacg gccatggcgc agcccagcag
       61 atctgccgag aactattttg acgccgccaa tgaactgtac cttagcggtc gtgactttcc
      121 agatgcggcg caatatttgc agaagtatct cgattctggt ggactggtgg agaatgcgcc
      181 gccgttccgc gcccactacc tgctaggtca gatctacgag aagatgggcc gtagttcgga
      241 cgccacctcg gagtacgagg cgtcacttgc tctggcgtcc ggtttcgacc gcgcgcggaa
      301 agctcttggc cggctacagt agccgtcttc actcatttca ccaggtagag ctttaaggat
      361 gcccccaacg ccccgctgtt tgagaaccac tcgggttgac tgtgctgtga gggtaaccgc
      421 tctcacctgc gcactcgtgc tttcatgtgc tgcttgcgtt gcagccgatg ctgcgactgg
      481 cgaaccgatt tctttccgta gagcggtgga cttggcgctg cagcacagcg gtgtcatggg
      541 tatcgcgacc gtcaaccaat ggcgagcgcg cgaggcctat gaggaggttc gggcgaacta
      601 tattccgcag gtcaccattg gttccggcct cggttactcc tatggatttc cgctcaccct
      661 ggaaggctcg gcgccctcgg tggtgaactt caattccgcc cagtcgttat tcaacctctc
      721 gctacggcaa ttcctcaaag ctgcaaagat cgactggaat gcaagttccc tggacgtaca
      781 agacaagcgc gactccgtca ttctggatac cgcgttgacc tacgcgcaac tcgatcagct
      841 caccggcaag ctgaaggcgt taggggaagc tcaaagcgcg gccgacaacg cacaatttgt
      901 cactgaacag cgcctgcagc agggcgtgga cagcaagctt gatctcacga aaagtcagtt
      961 ggtggcggct cgcattcgat tgcgaattgc cgaggcgcag gggcaagccg acgtgctgcg
     1021 cgagcatctc tccaagctgc tgggattacc tgccgagtcc atcgcggtcg aacctgcatc
     1081 gatgcctgag ctgcctgtga tttcgcagga tgacaatctg ccggcccgcg ctgtggagaa
     1141 cagtctcgcc gtgaagcagg ccgaagagag agtggccgct gcacaggcgc gagctaccgg
     1201 cgaacacaag gcgctgaaga tgcccatcat cgatttggca tcgcaatacg cttatctggc
     1261 caaatacaat aactacgatc tctactatcg caactacact gccaacaatt tcagcggtgg
     1321 cctgaatatc aggttcccca tcttcaattc ggcgcagaaa gccaaggcgg agcaagctga
     1381 tgccagtgcc atgatcgcaa agaagcaggt agagatcaca aagaaccagg tcagcgaaga
     1441 tgccttgaag ctccagaggt ctctgcgcca gttggccgca gcacgagatg tgtccagatt
     1501 ggaatgggaa gtcgctcaag gagatctgga agcggtgaag gcccgaatcg agaccggcaa
     1561 cggcaacacc cgcgatcagc aaaacgcccg cctcgatgcc gaagataagc acgcggccta
     1621 ctttgacgct cagctcgaag tcagccgggc cgaactgcaa ctgctccggc ttaccggcga
     1681 acttgaaaaa tgggccattc cgcctcgcta atcaaccgtt gactgccagc cataaaatgt
     1741 atcccgctgg cagtgaaatg tatctaaccg aaacacaggt aagaagttcc gccagcaagc
     1801 ggcaggtgat acgatagtta atcctatgcc tttgaaaact ctgtttttga atcccccttc
     1861 gtttgagaac tatgacggcg gcgccagttc gcgctggcca gccacgcgcg agatcgagtc
     1921 ctattggtac ccggtctggc tggcttaccc ggcaggcatg ctggaaggct cccgcctgct
     1981 cgacgcttcg ccgcacaagg tgacggcgca agagaccatc caaattggaa gggattacga
     2041 atttctggtt ttgtttacca gcacgccggg ttttcagggc gacatcaagc tggcggctgc
     2101 catgaaagcg gccaatcctg ggctgaagat cgctttcgtg ggaccgcacg taaccaccct
     2161 gccggaacca tctctcaagg atggtcaggc gatcgatttc attgtgcgcc gggaattcga
     2221 ttacgccgta gtcgagtatg ccaacggcaa gccattgaat gagattctgg gcgcttccta
     2281 ccgcggcccg aacggccaga tactccataa tccggaccgt ccggcggttg cagatctgga
     2341 tgctctgcct gatgtcgtgg atgtgtatag gcgcgacctc gatcctcgcc gttacagcgt
     2401 gcccttcctg ctgtatcctt tcgtctcgct gtacacgaca cgcggctgcc cggcgcagtg
     2461 cacgttctgc ttgtggccac agacgctcag tggacacccg tggcgcaagc gctcgacaga
     2521 cgcggtggcg gcggaaatgg cgaaggccaa ggaatactgg ccgtgggtga aagagttttt
     2581 cttcgacgac gacaccttca acattcagaa ggcccgtacc atcgaacttt gcaccaagct
     2641 gaaaccgttg aagctgacct ggtcttgcac ctcacgcgtc accaccgatt acgagacgct
     2701 gaaggcaatg aaagagtccg gctgccgcct gttgatcgtg ggctacgagt cgggcgacca
     2761 gcagatcctc aagaacatta agaagggcgc gaccgtcgaa cgggcgcggg acttcaccag
     2821 ggactgcaag aaactcggcc tcatcgttca cggcgacttc atcctcggtc tgcccggcga
     2881 aaccaaggaa accatccggc gtaccatcga tttcgcgaag gaactggacg tcgataccat
     2941 tcaggtatcg ctggcgcatg cgtatccggg aacggaactc tacgattttg ccaaggcaaa
     3001 cggcttcctt aaggcagaag gctcccagat ggtcgacgcc atgggccatc aggtcgccat
     3061 gattgagtat ccgggcattt cccgagacta catcctggaa atggtgcacc gtttctacga
     3121 cgagtactac ttccgcccca aggccgtgtt ccggatcgtc aagaaggccg tattcaactc
     3181 gtcagaacgc aagcgcctgt acaaggatgc gaagtcgttc ctcaagctgc gcgccgcgcg
     3241 taatcgttcc gtaaaggagt ctcgcaatca acccggcgct atcaacccac ctgcgcctcc
     3301 agtggggaca tcgaaagctg gcgctaaatc cgagacggtt gaaccggtca gcgcctaggg
     3361 gttggcgtga cctttcgtaa gtatcttgtc ctgttcgcgg tcgtgctctt cggcgcctgt
     3421 ggcgatgttt gcctcgcgcg cgggatgaag gattttgggg cggtaacttc cgcgaactgg
     3481 cgccatctgt ttgcttccct gtggaatccc tgggtgctga gcggcatctg tttgttgctg
     3541 ctgttcttgt gcagctacct cagcgcgctc tcctgggccg atctgacgta
//
LOCUS       DASRKK010000093         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1178542, whole genome shotgun sequence.
ACCESSION   DASRKK010000093 DASRKK010000000
VERSION     DASRKK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178542"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1129
                     /locus_tag="VFC15_01970"
     CDS             188..1129
                     /locus_tag="VFC15_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08957.1"
                     /translation="MSEAALDFASIRSSSAVEIISPVPPQSSNPTAMSRLEGRPVVRF
                     PEQLRLHRSLEDLSWTGVIDEFNDAARMTNPSVTEPILITTNGTILAGFGRWRLAVFD
                     GRHEINCIEYPLSEDEALQFILTHHQTRCGWNAFVRIRLALNLEPYFQQRALDNMCAG
                     GKYKGWANLPEAQHIEVRQEIARAAGVCARNVSNVKTILQTAHPRLKEALRDGTLTIN
                     RAIQFCKLPRAEQLEQFIRYSEERATNKVIRQSIARPKEDKTSLDVVAVLDALQQQEA
                     RQPGSVAVRVGRHKRTVVLIGQDLCVEPRSQKELKLT"
     gene            1126..2229
                     /locus_tag="VFC15_01975"
     CDS             1126..2229
                     /locus_tag="VFC15_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM08958.1"
                     /translation="MKYHHPLKKILSQARRYWDRDEMRPAIRRVFGKALQCRTADLGA
                     EVYCSEKQELILYHTCKSRACPSCGYRAQVQWLRERWVALPDVLYKGVTFTMPNQLWP
                     LFRDNPPVAKTLSALAAEVIKARMGAKYGLRVGVIAILHTFNGKLEFNSHAHTMVTGG
                     GLHGSSDIWVSSVYYERDPLMEAWRRAVIALLRAALRAGQLHTKMNVAQMEELLTQQE
                     KCSWIIKIQSFEDKWHFLQYAGRYVRRPPIAQRRITWIGERTVRFWFKDKKLRRRVEE
                     QCSLEEFIDRWSQHIPARYQHAVRSFGLFAPRALGQTSAAIFVILRQERKPRPKPRRW
                     AESIKRDFKHDPLLDHMGKKMKWVRRLAPTASR"
BASE COUNT          659 a          826 c          787 g          595 t
ORIGIN      
        1 caactgatat atataccgct gctccctggc cctaccgtcc gcgcggaggc tcaccaatga
       61 attgggaatc ggacactcaa agtgcaagac gaaacatctt ccctcaaccc ctggttgata
      121 cacagctaac ctcggaagaa cgcggcaaag tccggcaaaa tgcggcggtg caagaagagt
      181 gcctgcgatg agcgaggcgg cgctcgactt cgcgtccatt cgcagttcaa gcgccgtaga
      241 gattatctct ccagtcccgc cgcagtcttc caatccgacc gcgatgagtc ggttggaggg
      301 gcgtccggtc gttcgtttcc cagaacaatt gcggctgcat cgctcgctgg aggatctcag
      361 ctggaccggt gtaatagacg agttcaacga cgccgcccgg atgacgaacc cgtctgtaac
      421 cgagccgatc ctgatcacga cgaacgggac gattctcgct ggctttgggc gctggcggtt
      481 agcggttttc gacggcaggc acgaaatcaa ctgcatcgag tatccgctca gtgaggacga
      541 agccctacag ttcatactca cccaccatca aacccggtgc gggtggaatg cgtttgtccg
      601 cattcgcctg gcgctgaatc tggagcccta cttccagcag agggcgctcg acaacatgtg
      661 tgccggaggc aagtacaagg gttgggcaaa tttgccggaa gctcagcata ttgaagtgcg
      721 ccaagagatt gcccgcgccg cgggggtttg cgcccgtaac gtcagcaatg tcaaaacgat
      781 cctccagacc gcacatccta gattgaaaga agccttgcga gacggcacgc taacaatcaa
      841 ccgcgcgatc caattctgca aattgccccg ggccgagcag ctggagcagt tcatccgtta
      901 cagcgaggaa cgcgctacga acaaggtaat ccgtcagtct atcgctcggc ctaaagagga
      961 caagaccagc ctcgacgttg tcgccgtact cgacgccttg cagcagcagg aggcgcgaca
     1021 acccggatcg gttgcggtac gggtcggccg tcataaacgc accgttgttc ttatcggtca
     1081 agatctgtgc gtcgaaccgc gttctcaaaa ggagctgaag ctgacatgaa ataccatcat
     1141 ccgctcaaga agattctgtc ccaggcacgt cggtactggg atcgagatga gatgcgcccc
     1201 gcaattcgca gggtttttgg caaggcgttg cagtgccgca ctgcagactt gggtgccgag
     1261 gtttactgtt cagagaagca ggaactcatc ctctaccaca cgtgcaaatc gcgagcctgc
     1321 ccgagttgtg gctaccgcgc ccaggtacag tggctgcgcg agcggtgggt cgcactgcca
     1381 gacgtgcttt ataagggggt cacattcacg atgccgaatc aactgtggcc ccttttccgc
     1441 gacaacccgc ccgtggctaa gactctgtcc gctctagccg cggaggtcat aaaggcacgg
     1501 atgggtgcga aatatggttt gcgcgttggc gtcattgcca ttctccacac cttcaacggg
     1561 aaattagagt tcaactcaca cgcacacaca atggttaccg ggggagggct gcacggttcc
     1621 tccgacatct gggtgtctag cgtctactac gagcgcgacc cattgatgga ggcttggcgc
     1681 agagccgtga tagcgcttct ccgcgcagcc ctgcgagcgg gtcagcttca cacgaagatg
     1741 aacgttgccc agatggaaga gctgctaacg cagcaggaaa aatgcagttg gataatcaag
     1801 atccaatcat tcgaggacaa atggcatttc ctgcaatacg ccggtcgcta cgtaaggcgg
     1861 cctcccatcg cgcagcgccg tatcacctgg atcggagaac gaaccgtcag gttttggttc
     1921 aaagataaaa agctgcgccg tcgtgtcgag gagcagtgct cactggaaga gtttatcgac
     1981 cgttggtccc aacatattcc agcccgttat caacacgcgg ttcggagctt tgggctgttt
     2041 gctccgcgcg cgctcgggca aacctcggca gccatctttg tcatcctgag acaggagcga
     2101 aagccacgcc ccaaacctcg ccgttgggcg gagtctatca agcgagactt caaacacgat
     2161 ccgctcctcg accacatggg caaaaaaatg aaatgggtgc gacgattagc gccgacggcg
     2221 tctcgctaat caaaactcag ccgaaaatgc catcacctta ctgccgcaat ccgcgatggg
     2281 agacgatggc tttccctctc cctcccgctt tttaggctga acagccacgg tttcgcccgt
     2341 aggacgatct gagttgttgc tttcgaccga caatgtcgat tcgacccggc aataaatgcc
     2401 acgttcacac ttaacacgag attttgattt tcctatactt caagattccg ggcaggctgt
     2461 tcaggggtgc attccgaagg ttaaccgaat tgacgaatgc ggagtcggtg gccttgtaaa
     2521 cgtacccatc aatcagcgcc actggccttc ttcttactgc gggttcccac cgaattactc
     2581 atttcagcta actacgtccg cagacgcacc tcaagaatgc gtcgctgatg gcgaacatga
     2641 gattttgata ttcctatact ttgaaggtaa ggtgttacgc gacactggct cagtatgtcc
     2701 atgccaagcg caagacggtg gagcagcgcc cagcatgacg accagcggat ttctcgcgtt
     2761 ggcaagtggc tgggcgcatt cggcccgtcg agtggccgca actcttcaac ctcgtcaaac
     2821 gcaatatttc tttcgtagga ccgcgcccgg ttccgccgca tgaaaat
//
LOCUS       DASRKK010000094         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_787712, whole genome shotgun sequence.
ACCESSION   DASRKK010000094 DASRKK010000000
VERSION     DASRKK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787712"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1062)
                     /locus_tag="VFC15_01980"
     CDS             complement(106..1062)
                     /locus_tag="VFC15_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM08959.1"
                     /translation="MFMITIFLLLLVSVFATVAYFTEPSEADKRTRERLVGLDRHTSM
                     EDRKDDIVRQVTFSSVPSVDRFLRNNKYAIRLQLMLDQAKLSWTVGRFFFFSAVLMLI
                     GAAIGNWWIPADFFGWIPGIALGTIPLAWVMYRRAGRFRRFNLTLPDAIDLMSRALRA
                     GHALPSALSMVADEISDPLGPEFRRTADELNYGLPFREALLNLERRFPVRDLRFLVTA
                     ILVQKESGGNLITLLDKSAAVLRSRIHLAQKVRVFTAQGRLTGVILMALPFVCFIALN
                     LVSPGYTKPLFENEIGRRMVYGTILSLAIGLFLIRRIIRIKV"
     gene            complement(1077..2426)
                     /locus_tag="VFC15_01985"
     CDS             complement(1077..2426)
                     /locus_tag="VFC15_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZM08960.1"
                     /translation="MANGKNFAPDMRLGRSWTSARSMGVSPRTYQDLKSTLHRDLLDL
                     IDLEKLTSLEDIKARVQVQEVIHELMAKSNTPLSTSERERLSREVLHEVFGLGPLEPL
                     LQDPTINDILVNTHKKVYVERAGVLEKTNVMFKDAAHLRHIIDKIVSNVGRRVDESSP
                     MCDARLTDGSRVNVIIPPLAVDGPILSIRRFGKIPLGSEQLLGNRTITGPIFEVLKGA
                     VRARLNIVISGGTGAGKTTFLNVLSGYISEAERIVTIEDSAELQLQQEHVVRLECRPP
                     NLEGQGAVRQRELVINALRMRPDRIIVGEVRGEEALDMLQAMNTGHDGSLTTIHANSP
                     RDAVARLETMSLMSNLNLPEKALRQQIASAISIIVQICRMSDGTRRITHVTEITGITT
                     DVVAMQDIFVFEQLGVDAGGRVLGRFKATGVAPKFAERLKTSGIVLPPSIFEHSFVV"
     gene            complement(2430..>3748)
                     /locus_tag="VFC15_01990"
     CDS             complement(2430..>3748)
                     /locus_tag="VFC15_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM08961.1"
                     /translation="VRQRLAEPCLAKPCQESGDKAMMTDEKVVLQGSRRSEQRIGVMC
                     IGMDAGSREGLDVLVAQTPGAYVVDNVDWHITPREAMKMLEPYQHRICVIDFDDGTEK
                     GCRVAQRLHDGCDSSLGIFAAAGGSDPEQIIAAMRSGCSEYLVKPFQPERISHALAHV
                     AARPQGKSGDLVKGRIMTVMGAKGGTGATSLALHLALNLVQRHGQKCLLVDEHPAFGD
                     ASLYLGLGRHQYSFYELVHNTDRLDADLLRGFLLRHPSGLDVLDSPEAIDAFPHPSPE
                     AIEHTLSFLGENYQFVIIDCPPGLTEESCAAIRQSDRLAIVITPELPAIRNALRSIEY
                     LTSMHYSADSIDIVLNRHSKKSALSDHEVEAALHRPIAVRIPNNYGEIVNAINAGTPI
                     EFGHRSELPAAFDYWAVRLLGNEADIPSANKGPRGWFGIFGSRVEG"
BASE COUNT          820 a         1102 c         1036 g          790 t
ORIGIN      
        1 cttgcctcgg tcggcgactg ttcccccgcg acagcaagaa taagcagggc actcacagca
       61 agcagtgcca cgccagcaaa aatcgcaatg aatagcacac tcatatcaga ccttgatccg
      121 aatgatccgc cgaatcagga acaacccaat cgccagactc agaatcgtgc catagaccat
      181 tcttcggcca atttcgttct caaacagcgg cttggtatag cctggcgaga ccaagttcag
      241 ggcgatgaag cagacaaatg gcagcgccat caggatgacc ccggtgagcc ggccctgagc
      301 agtgaatacc ctgactttct gggccaggtg aatgcgcgat cgcagcaccg ccgcgctctt
      361 gtcgagtaac gttatgagat tccccccgga ttccttttgt acaagaattg cggtcaccag
      421 gaaccgcaag tcccgaaccg gaaaacgacg ctcaaggttc agcaaagctt cgcgaaaggg
      481 cagtccataa ttcagctcat ccgcggtacg gcgaaattcc gggcccagcg gatccgagat
      541 ctcatcggca accatggata gggcgctagg cagcgcgtgt ccggcacgaa gagcgcggga
      601 catcaagtcg atggcgtcgg gaagcgttag attgaagcgg cggaaacggc ccgcgcgacg
      661 gtacatgacc caagccaatg ggatagttcc caacgcgatt ccagggatcc acccaaagaa
      721 gtccgccggt atccaccaat tgccgatcgc cgcaccgatc aacattagaa ctgccgagaa
      781 aaaaaagaac cggccaaccg tccaggatag cttggcctgg tcgagcatta attgcagcct
      841 tatcgcatat ttgttgtttc gcaggaatcg atcgacgctg gggacactgc tgaacgtgac
      901 ctgtcttacg atgtcatctt tgcggtcctc catactcgta tgccggtcca ggccaaccag
      961 gcgctcacga gttcgcttgt cggcttctga aggctccgtg aaatacgcga ccgtagcgaa
     1021 cactgacacc aaaagaagca gaaatatcgt gatcatgaac atacgtgtgc tccggtctac
     1081 accacaaatg agtgctcgaa aatgcttggc ggaaggacga tgccggacgt ctttagtcgc
     1141 tcggcgaact tgggcgccac ccccgtagcc ttaaaccgtc ccaatacgcg tccgccggcg
     1201 tcgactccga gctgctcgaa gacgaagatg tcctgcatgg caaccacgtc agtggttatg
     1261 ccggtgattt ccgtcacgtg ggttatgcga cgagtcccat cgctcatgcg gcatatctgc
     1321 acaatgatgg aaatcgcaga tgcgatctgc tggcgaagcg ccttctccgg cagattgagg
     1381 ttggacatca gggacatggt ttccagtcga gccactgcat cgcgcgggct attggcgtga
     1441 attgtcgtta acgacccatc atgtccagtg ttcatcgcct gcaacatgtc gagggcttcc
     1501 tctccgcgta cctcgcccac gattatcctg tccggcctca tgcgcagtgc gttgatgacc
     1561 agttcgcgct gacgtacggc tccctgtcct tccaagttag ggggccggca ttcaagccga
     1621 acaacgtgct cctgttgcaa ttggagttcg gcagagtcct cgatggtgac gatacgttcc
     1681 gcttcgctta tatatccgct gaggacattt agaaaagtgg tcttgccggc gcccgtaccg
     1741 ccggagatga cgatgttaag ccttgcccgc acggcgcctt tcaagacctc gaagatcggc
     1801 ccggtgatgg tgcggttgcc gagaagctgc tcactcccca aggggatctt tccgaaacgg
     1861 cggatcgaga gaattgggcc atcgaccgcc aagggaggaa tgatgacgtt gacgcgcgac
     1921 ccgtcggtca ggcgcgcatc gcacataggc gacgattcgt ccactcgcct gccgacgttg
     1981 gaaacaatct tatcgatgat gtgccggagg tgggccgcgt ccttgaacat gacgttcgtc
     2041 ttttccaaaa cgccggcacg ctcgacgtag acctttttgt gcgtattaac caggatgtcg
     2101 tttatggtgg ggtcctggag caaaggctcc aagggcccca atccgaatac ttcgtgcagc
     2161 acttcccgcg acaggcgctc cctctcgctt gtgctgagag gtgtattcga tttcgccatg
     2221 agctcgtgga ttacttcctg cacctgcacc cgggccttga tgtcttcgag cgacgtgagc
     2281 ttctccaggt cgatgagatc cagcagatcg cgatggagtg tggacttcag gtcctggtaa
     2341 gtcctgggag agacacccat gctgcgtgcc gaagtccaac tcctacccag gcgcatgtcg
     2401 ggagcaaagt tctttccgtt ggccattttc tacccttcaa cccgcgatcc gaagatgccg
     2461 aaccacccgc gaggaccctt gttcgcgctt ggaatatcgg cttcatttcc gagcagtcgc
     2521 accgcccaat aatcgaaggc cgcgggaagc tccgatctgt gaccgaactc gatgggggtg
     2581 cctgcattga tagcgttcac aatctcccca tagttgtttg gaatccttac ggcaatcggc
     2641 cgatgcagtg cggcttcgac ctcgtggtca ctcaacgcac tcttcttcga atgtcgattc
     2701 aacacgatat cgatgctgtc tgccgagtaa tgcatgctcg tcagatattc gatcgagcgg
     2761 agtgcgttac gaatcgctgg aagttccgga gtgatgacga tggctagacg gtcggattgc
     2821 cggatggcag cacagctctc ctccgtcagt ccaggaggac aatcgataat gacaaactgg
     2881 tagttttccc cgagaaacga cagcgtatgc tcgatggcct ccggcgaagg gtgcgggaat
     2941 gcatcgattg cctcgggaga atccaatacg tccaatccac tcggatgccg tagtaaaaac
     3001 cctcgcagca ggtcagcatc gaggcggtcc gtgttgtgca ccaattcgta gaagctgtat
     3061 tgatgacggc ctaatcccag gtaaagcgag gcatcgccga acgcgggatg ttcatcgacc
     3121 agcaagcact tctgtccatg tctctgaacc aggttcagcg ccaaatgcag cgccagagag
     3181 gtagcgccgg tccctccttt ggctcccatt accgtcatga tgcggccctt cacaaggtcg
     3241 cccgacttgc cttggggccg cgccgctaca tgcgccaacg cgtgcgatat ccgctccggc
     3301 tgaaacggct tcaccagata ttccgaacat cccgaccgca tggctgctat gatctgctca
     3361 ggatcggagc cacccgcagc ggcgaaaatc cccaggctgc tgtcgcagcc gtcgtgaagc
     3421 cgttgggcga cgcggcagcc tttctccgtg ccgtcgtcaa aatcgatcac gcagatgcga
     3481 tgctgatacg gttccagcat tttcatggct tcgcggggag taatgtgcca atcgacgtta
     3541 tccacgacat aagcgccggg cgtttgcgcc accaggacgt ccaggccttc ccgcgagccg
     3601 gcatccatgc ctatgcacat cacaccgatg cgctgttcgg accggcgcga accttgtagg
     3661 accactttct cgtcagtcat cattgctttg tccccggatt cttggcaggg cttggcaagg
     3721 cagggctcgg caagcctttg tcgaacga
//
LOCUS       DASRKK010000095         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_690059, whole genome shotgun sequence.
ACCESSION   DASRKK010000095 DASRKK010000000
VERSION     DASRKK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690059"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1068
                     /locus_tag="VFC15_01995"
     CDS             283..1068
                     /locus_tag="VFC15_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM08962.1"
                     /translation="MATGPRRYDFREDNAEANVSIWKEALFAADILLLHSSPVYYGLG
                     VARGDGSAVILVPGFLGTDAYLSQLYTWLGRIGYRPYFSGIGMNAECPNILIKQRLNS
                     CIEKALLETEQKIHILGHSLGGIIARSVAGQRPEDVASVITLASPFRGTVAHRTVLQA
                     AEAVRKHILKEHGSGVLPTCYTSRCTCNFIDSLRRSVPDSVLETAIYTRNDGIVDWRY
                     CLTGNIDSDFEVPGTHIGLSFNSSAYTIIANRLAEARSRKPTA"
     gene            1341..2036
                     /locus_tag="VFC15_02000"
     CDS             1341..2036
                     /locus_tag="VFC15_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08963.1"
                     /translation="MRRSTFITLLLFLSVSVITCFSQQTYVSRYSAYGAYSYLSTPSL
                     NLTQRGFDGDFGINLRSWLTLGGDFSYAGGHSSLLPNMLNAATQAKLAPYVPVFQQLG
                     IPLAVPYNSATYTYEAGPQFNYRKVKKVTFFVRPALGALHANFEAKPNNPYIAKLVNG
                     LLNGSTSQSDTVVFYGFGGGFTYEATPHFGIRVASDFVHYNFFSNLLDGGRNSVRVTI
                     GTKFGWGKNILGK"
     gene            2383..2976
                     /locus_tag="VFC15_02005"
     CDS             2383..2976
                     /locus_tag="VFC15_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZM08964.1"
                     /translation="MLKRTLVVVLLGIIGIILFRHAASFLVVNDPKPADLILVLGGGE
                     NDLRYWNGVRLMQEGYAPRLMLDVFSRGQTFGRLDIDLAQELLDRTTPGQSTICPTRE
                     SSTYDEARYLGECLAGSDVKSILVVTSEYHTRRARDIFQHRLPQYHFSFYAAPDPYYF
                     GARWWTNRQWAKTTLSEWERYGWWLLVDRWRSGLVVH"
     gene            complement(3044..4375)
                     /locus_tag="VFC15_02010"
     CDS             complement(3044..4375)
                     /locus_tag="VFC15_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HZM08965.1"
                     /translation="MDQLIPTQQPSGSAKQPPLVDANRLRRLQSLGIICGFGAGAWLG
                     AAEAPTKLVAVGISPVVVSLLMVAGVFLARWSVPALVRGTGQIGFDLRQAPHLVVWGV
                     LAGCLWAVANTLTIFAIRDVGLSIAFPLWNMNSLIGILWGITLFSELRNAGARRWLSV
                     LGGALLMFLGACLLAYASSAQTTPEHATRGIVAALAAGVLWGTMYIPYRKAYLTGMNP
                     LSFVAFFTIGELVTMTILALTYGGGAAPLLRELVKAKDVLFWLLLGGFVWVIGDVFQQ
                     YAAKYVGISRGIPLSNTNQLWGMAWGILVFGELRGRGLSLYAQVIGGSLLMAAGAGAI
                     ALSSVTGKEHGRWHEAAHREGKRYGVSAEYVQAGLAGKDANLQTAGKRPRTWLDWSIV
                     AIATAIFIGLAVISRVPDISLNWTAISLLSAILLIFLAACGTALWKATRFS"
     gene            complement(4363..4527)
                     /locus_tag="VFC15_02015"
     CDS             complement(4363..4527)
                     /locus_tag="VFC15_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08966.1"
                     /translation="MQDCSAALRGGKFMRDVGVPGRWQSVAGVVLRDVHFWIPVLVLL
                     GGSAVLRWIS"
     gene            4646..6331
                     /locus_tag="VFC15_02020"
     CDS             4646..6331
                     /locus_tag="VFC15_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM08967.1"
                     /translation="MSELPLPLPPLSALDARTQAFIKLTAAQIDRTRPFGNVRPVAVG
                     EVLFEVGDTSIPMFVLLSGEMEIVQPHCEGERFVAKHEPGEFTGETTMISGRRALVRG
                     RITKAGEVLEISPENLRSLIAKDAELSEIFMRAFILRRLALIATGFGDAVVLGSRHSA
                     NTLGLREFLARNGHPYTYVDLDTDSHAQELLDRFNVTINEIPVLICRGSAVLRNPTVQ
                     QVADCLGFNSPADEARIRDVIVVGAGPSGLAAAVYAASEGLDVLLIETNAPGGQAGAS
                     SKIENYLGFPTGISGHELATRALHQAQKFGAEMMVAHGAVRLLCGSKPYQVALDNGQK
                     LTTRAVVIATGAQYNKPSIPNLAKFAGLGVYYGATFMEAQLCEGEDVIVVGGGNSAGQ
                     ASVFLSQTARKLHVVVRSGQLSDTMSRYLVQRIEENPKITLHYHTEIVELEGDLQLER
                     VQLRNKVSGEVETLPIRHVFMMTGAAPNTTWLNDCLALDGKGFVLTGHDLEVHPSHFS
                     QWPGLRPPFLLETSMPGVFAVGDVRSGNVKRVASAVGEGSIAVQMVHRVLAEV"
     gene            complement(6349..6954)
                     /gene="leuD"
                     /locus_tag="VFC15_02025"
     CDS             complement(6349..6954)
                     /gene="leuD"
                     /locus_tag="VFC15_02025"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZM08968.1"
                     /translation="MEPIRKHRGVVAPLYRENVDTDQIIPKQHLKRIERTGFGQFLFF
                     DWRYHPDGRDREDFVLNHEHFRTATVLVAGKNFGCGSSREHAAWALGEFGFRVILAPS
                     FADIFRNNAGNNGLLLIELAEPVIQLIAEHADKHRPYHLSVDLENQTVSDDFGLSESF
                     AIDAFRRHRLLNGLDEIGMTLEHEADISRFERERLTRATQG"
BASE COUNT         1504 a         2128 c         2002 g         1474 t
ORIGIN      
        1 cctgcacttt catcgcccgc tccatcgcgg ccttcgagta aaccaccatg ctatgcatgg
       61 tctcctactc tcgccgcccg caaagcggac agatcacgtg ctatctcaac cggacatatc
      121 atgtgctacc gacacagctc aaagttaacc tttgcaactt cgagcaaaag gtctacacta
      181 actccccgag gacggtgcag acagtttctc ctgcctctgt gcgggtaccc cccgtgactg
      241 caggcgaccg aacccacgtc tgagtcccag gagaaggtgg atttggcaac gggcccacgg
      301 cgctacgact ttcgcgaaga caacgcagag gcaaatgttt ctatttggaa ggaagcgtta
      361 tttgccgctg acattctcct cctccattcc tctcccgttt attacggcct tggcgtcgca
      421 cgcggtgatg gatcggcagt aattttagtc ccgggatttc ttggcacaga tgcgtaccta
      481 tcgcagctct acacctggct gggacgcatc ggatatcggc catacttttc cggcatcggc
      541 atgaatgccg aatgcccgaa tatcctgatc aaacaacgcc tgaattcatg tatcgagaaa
      601 gccctgctgg agacggaaca gaaaattcat atcctcggtc acagcctggg agggattatc
      661 gcgcgctcgg tcgccggtca aagacccgaa gatgtggcat cggtgatcac tttggcctct
      721 ccattccgtg gcaccgtagc ccatcgcacc gtgctgcagg cggcagaagc ggtgcgcaag
      781 catattttga aggaacacgg ttcgggagtc ctgccaactt gctacaccag ccgatgtacc
      841 tgtaacttca tcgattccct tcgccgcagc gttccagact cggtgttgga gacagccatc
      901 tacacccgca acgatggcat cgtcgattgg cgttattgcc tgaccgggaa tatcgattcc
      961 gattttgaag ttccgggaac ccacatcggc ctgtcgttta actcttccgc gtacaccatc
     1021 atcgccaacc gtctggccga ggcacgctcg cgcaaaccga ctgcgtaacc gcgattgcgc
     1081 tccgctcgcc ggcgcggagt gtcggacgag ctgaacttct cttcaagcca cggaacaggc
     1141 tcggcgccca aataattccc atttcgggaa tatttccctc aaatggcacg aatttgctat
     1201 tagcacaggc cgcaaatgac agacacttaa gctgtcgtgt tcggtagccc gaatggaccc
     1261 tgatggcagt gcgcgcacaa gcgacgatct gccgaactcg acgggacccc aaatcaatca
     1321 agctaaccag gagaagccct atgcggcgtt ccactttcat aaccttgctc ctctttctgt
     1381 ccgtttctgt gatcacgtgt ttctcgcaac aaacctacgt tagccggtac agcgcttatg
     1441 gcgcgtactc ctacctttcc acgccgtcgt tgaatttgac gcagcgcgga ttcgatggcg
     1501 actttggtat caaccttcgc agttggctga cgctcggcgg cgatttcagc tacgccggtg
     1561 gccactcttc gctgctgccg aacatgctta atgcagcaac acaggcgaaa ttagcgccgt
     1621 atgtaccggt cttccagcag ttggggatcc cattggcggt cccttacaac tctgcgacct
     1681 atacctatga ggcgggtccg caattcaatt accgcaaagt gaagaaggtg accttctttg
     1741 tgcgtccggc gctcggggca ctgcatgcga attttgaggc caagccgaac aacccataca
     1801 tcgcaaagct ggtcaacggc ttgctgaacg ggagcacgtc gcagtctgac acggttgtgt
     1861 tctacggctt tggtggtggg ttcacttatg aggcgactcc gcacttcggt attcgtgtcg
     1921 ccagtgactt tgttcactac aattttttct cgaatctgct ggatggcggt cgtaacagcg
     1981 tacgcgtcac gataggaacg aagttcgggt ggggaaagaa cattttaggg aagtagctga
     2041 gggatgattg gccgactgcg atttcgtttg cgtggcatgc tgcccgtgta cgaaatcgca
     2101 gcctaccttc gctgcgtcgc acctcagcaa cgccgcagtg ctgcccagca gattccagac
     2161 gggcaggatc ggctctgatc ggcgcaggca ggcgagatgc gctgcgtggt tcgtcagatc
     2221 cgatgtggct ggcaagtcca gccgaccgct tcatagtgtg tccgctcccc tttcacccgg
     2281 acgcgcaatc cggtccatcg ctgactttcc cgcatgtaaa gcgggcttcc ggcctaccgc
     2341 atgcacggtg gcaatacccc gtcggataaa ctcagtacga tcatgttgaa gcgaaccctg
     2401 gtagtggtct tgcttggaat catcggaatt atcctcttcc ggcacgccgc gtctttcctg
     2461 gtggtgaacg atcccaaacc cgccgatctc attctggtgc tgggcggcgg cgagaacgat
     2521 ttgcgctatt ggaacggcgt caggctcatg caggagggct acgctcctcg tctcatgttg
     2581 gatgtttttt cccgcggtca aacattcgga cgtctcgaca tcgatctcgc ccaagaatta
     2641 ctggatcgga caaccccggg gcagtctaca atctgcccta cccgagagag ctcaacttac
     2701 gacgaggcgc gctatctggg agaatgcctg gcgggctcgg atgtcaagtc gatcctggta
     2761 gtcacttcgg agtaccacac tcgccgcgct cgcgacatct tccaacatcg gcttccccag
     2821 tatcattttt cgttctatgc cgctccggac ccctactatt tcggcgcgcg atggtggaca
     2881 aatcgccagt gggcaaagac cacgctgtct gagtgggagc gctacgggtg gtggctattg
     2941 gtggacagat ggcgtagcgg attggtggtg cactagcgca gttggttgtt tccaaatctt
     3001 ccaccatggc gctatcagca tccgtgagcg gcgattcgcg tcgctagctg aagcgagttg
     3061 ctttccacag agctgtgccg catgcggcca ggaagatcag cagaatcgcc gacagcaacg
     3121 agatcgcagt ccaattcaag gagatgtctg gaacccggct gatcacggcg aggccaataa
     3181 agatcgctgt ggcgatcgcg acaatgctcc aatccagcca agtgcgggga cgtttcccgg
     3241 cggtctgaag gttggcgtct ttgcctgcaa gacctgcctg gacatactcg gctgagactc
     3301 cgtaacgttt gccctcgcga tgggccgctt catgccatcg cccatgctct ttcccggtca
     3361 cggaggagag cgcaatcgcg ccggctcctg cggccatcaa caacgatccg ccgatcacct
     3421 gcgcgtagag tgacaggccg cggccgcgta actctccaaa aacaagaatg ccccacgcca
     3481 tcccccacaa ctggttggtg ttcgagagcg gaataccgcg gctgatgccc acatacttgg
     3541 cggcgtactg ctgaaatacg tcgccgatga cccatacgaa gccacccagc agcagccaga
     3601 aaagcacgtc cttcgccttc accagttcgc gcaaaagagg cgcggcacca ccgccataag
     3661 tgagcgcaag aatggtcatg gtaaccagct cgccgatggt gaagaaagcc acgaacgaca
     3721 ggggattcat tccggtcagg tacgccttac ggtacgggat gtacatcgtt ccccataaga
     3781 ctccggcggc caatgccgcc acgatgccgc gggtggcgtg ctcgggtgtg gtttgagcgg
     3841 acgaagcata tgccaacaga catgcaccta gaaacatcag cagtgcgccg cccagtacgc
     3901 tgagccaacg acgcgcacct gcgttgcgca attcgctgaa caacgtgatt ccccacagga
     3961 tgccgatgag gctattcata ttccacagcg gaaacgcgat gctcagcccg acatcgcgaa
     4021 tcgcaaagat agtcaatgtg ttggcgacag cccacaggca acccgcgagc actccccaaa
     4081 ccaccaagtg cggcgcctgt cgcaagtcga atccgatttg ccccgtgcca cgaactaatg
     4141 caggcacact ccagcgcgcg aggaacactc cagccaccat cagcaacgac acgaccaccg
     4201 gagatatccc gacagcaacc agtttggtcg gggcctccgc tgctccgagc cacgctccgg
     4261 cgccgaagcc gcagataatt cccagagatt gcagtctgcg gagacgattc gcatccacca
     4321 acggcggttg ctttgcggac ccgctcggtt gttgagttgg aatcaactga tccaacgcag
     4381 caccgccgat ccgcccagca gcaccaacac tgggatccaa aaatgaacat cgcgcagcac
     4441 tactcctgcg acactctgcc atcttcccgg cacacctaca tcgcgcatga acttgcctcc
     4501 tcgaagcgca gcggaacaat cctgcatagt tattatcaac aaccttgggg ccaggctggc
     4561 caacaaaggc aatcctgatt cgttccggca tttgggtgca tctacttagg gctgaaccac
     4621 ggaataaact cataaaggac tgctcatgtc ggaactacca ttaccgctgc cgccgctcag
     4681 cgcactcgat gctcgcaccc aagccttcat caaactaacg gctgcccaga tcgatcgcac
     4741 acggcctttt gggaatgtgc gtcccgtcgc agtcggggaa gtgctgttcg aagtgggcga
     4801 taccagtatc ccgatgtttg tattgctctc tggcgagatg gagatcgtgc agccgcactg
     4861 cgaaggcgaa cgcttcgtcg cgaagcatga accgggagaa tttacgggcg agacaaccat
     4921 gatctcggga cggcgtgcgc tggttcgcgg ccggattacg aaagcgggag aggttctgga
     4981 aatcagtccg gagaacctgc gatcgctaat tgccaaggac gccgagttga gcgagatctt
     5041 catgcgcgcc ttcatcctgc ggcgcctggc gctgatcgcc acgggattcg gtgacgcagt
     5101 ggtgctggga tcgcgccact ccgccaacac gctcggattg cgcgagttcc tggcgcgcaa
     5161 cgggcatcct tacacctatg tcgatctcga caccgacagc cacgcgcagg aattgctgga
     5221 tcggttcaac gtcaccatca acgaaattcc ggtgctgatc tgccgagggt cggctgtctt
     5281 gcgcaacccc acggtgcagc aagtcgcgga ctgtcttggc ttcaacagtc ccgccgacga
     5341 ggcccgcatt cgtgacgtga tcgtggtggg agcgggacct tccgggctgg cagccgcggt
     5401 ttacgcggct tccgaagggc tcgacgtgct gctcattgag accaatgctc ctggtggaca
     5461 ggcgggagcg agttccaaga tcgagaacta cctgggattt ccaacgggca tttcgggcca
     5521 tgaactggca acgcgagcgc tccaccaggc ccagaagttc ggagcggaaa tgatggtcgc
     5581 gcacggagcg gtccgcctcc tctgcggcag caagccatac caggtcgcac tcgataacgg
     5641 gcagaaactc acgacccggg cggtagtcat cgccaccggc gcgcaataca acaaaccatc
     5701 cattccgaac ttggcaaaat tcgcgggact gggtgtctat tacggcgcaa ccttcatgga
     5761 agcccaattg tgcgaggggg aagatgtcat cgttgtgggc ggcggaaatt cggccgggca
     5821 ggcatcggtg tttctttctc aaactgcccg caaactgcac gtagtggtgc gctccggaca
     5881 gctctcagac accatgtcac gctaccttgt gcaacgcatt gaagaaaacc ccaagatcac
     5941 cctgcactat cacaccgaga tcgttgagct tgagggcgat ttgcagttgg aacgggtgca
     6001 gttgcggaac aaggtttcgg gtgaggtgga gaccctgccg atccggcatg tgtttatgat
     6061 gaccggggcc gcccccaaca cgacatggtt gaacgattgc cttgcgctcg acggcaaggg
     6121 atttgtcctg accggtcacg acctggaggt gcatccttcc cacttctcgc agtggcctgg
     6181 attgcgaccg cccttcctgc tggaaacaag tatgcccgga gtcttcgcgg tcggcgacgt
     6241 gcgttcggga aacgtaaagc gcgtggcgtc agccgttggg gagggttcga tcgcggtcca
     6301 aatggtgcac cgcgtcctgg cagaggtgta gctcgcaaaa ctgtaaggtt agccctgcgt
     6361 agcacgggtt agccgctcgc gctcgaagcg cgaaatgtcc gcctcgtgct ccagggtcat
     6421 gccgatctca tcgagaccgt ttaatagtcg atggcggcgg aaggcgtcga tcgcaaagct
     6481 ctctgatagg ccgaagtcgt cgctgacggt ctggttttcc agatcgacgg acaagtgata
     6541 gggacgatgc ttgtcggcat gttcggcgat gagttgaatc accggttcgg ccagctcgat
     6601 cagcagcaat ccgttgttgc ccgcgttgtt gcggaagatg tcggcaaagg acggcgcgag
     6661 gatgacgcgg aacccgaact ctcccagcgc ccacgccgcg tgctcgcgcg aagatccaca
     6721 gccgaaattc ttgcccgcaa ccagcaccgt cgccgttcgg aaatgttcgt gattgagcac
     6781 gaagtcttct cggtcacggc catcgggatg atagcgccag tcgaaaaaca ggaactgccc
     6841 gaatcccgtg cgctcgatgc gtttgaggtg ttgcttgggg atgatctggt cggtatcgac
     6901 gttttcgcga tacagcggcg ccaccactcc acgatgcttg cgaatcggtt ccatcgcgtt
     6961 agttcctgta cttccagccg cgaacatcga cgaaccgccc ggccacggca gcggcggcgg
     7021 cggttgccgg acttactaaa tgggtgcgtc cttccctgcc ctggcggcct tcgaagttcc
     7081 ggttgctagt tgaggcacag cgctgctt
//
LOCUS       DASRKK010000096         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_787827, whole genome shotgun sequence.
ACCESSION   DASRKK010000096 DASRKK010000000
VERSION     DASRKK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787827"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFC15_02030"
     CDS             <1..336
                     /locus_tag="VFC15_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM08969.1"
                     /translation="DAIRKGEMRTWDDLREAIVNGAVKRLRPKIMTVACMLFGLLPLM
                     WSSGSGADVMKRIAAPMVGGIITSFLMELVVYPPVYAIWKWNWEVKPALKKDPAGVQL
                     TPANEVAHV"
     gene            329..1156
                     /locus_tag="VFC15_02035"
     CDS             329..1156
                     /locus_tag="VFC15_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM08970.1"
                     /translation="MCKALLAGAILVSAILGLSTAGAQTKLAAPASVQAPDQFSAFHK
                     ALDRAANDLLADAVQRMADAADDGQGGASRHSPESLVRDFDQKYRSDLTPSVGAAMKR
                     LDQLRPTLSRILETEGVPREIVSVVVVESGGRTTALSPKGALGLWQLMPDTARRYGLV
                     VTPTRDERLDIEKSTGAAAHYLRDLYQQFGSWPLALAAYNAGEQALQRAIARAGTRDF
                     VQISSLRLLPQETRNYVPAVLSAMQMLGVSYLPVEPRQIAKKGDSNGIIFAVAGAQP"
     gene            complement(1923..>2666)
                     /locus_tag="VFC15_02040"
     CDS             complement(1923..>2666)
                     /locus_tag="VFC15_02040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM08971.1"
                     /translation="VMPGIDGLELCRRIRERQTTPYQYILLVTAKDDAEDVVTGLEAG
                     ADDHLTKPFDRSELRARLRVGRRILTLQKGLIQAREELRFQATHDIPTGIWNRGALLD
                     LLQREIERASRSQSPTGLLMLDLDHFKKVNDTYGHLAGDVVLREVARRISEVVRSYDL
                     VGRYGGEEFLVVLPSCDKIQALQSAERIRSVIAERPVLAAGAEIVVTASIGVTVTTVS
                     TVAEKEILAIADTALYQAKSAGRNCTAAL"
BASE COUNT          584 a          780 c          746 g          556 t
ORIGIN      
        1 gatgccatcc gcaaaggcga aatgcgcact tgggatgacc tgcgcgaggc catcgtgaac
       61 ggcgccgtca aacggcttcg tcccaagatc atgaccgtgg cttgcatgtt gttcgggttg
      121 ctgccgctca tgtggtcgtc aggcagcggt gccgatgtga tgaagagaat cgccgcaccc
      181 atggtgggtg gcatcatcac gtctttcctg atggaacttg tggtttatcc gcctgtctat
      241 gccatttgga aatggaactg ggaagtgaaa ccagccctga agaaagatcc agccggcgtg
      301 caactcactc cggcaaacga ggtggcgcat gtgtaaggca ctccttgccg gcgcgatcct
      361 ggtctccgcc attcttggac tgtcgactgc tggcgctcaa acaaaactgg cagccccggc
      421 gtcggtgcaa gcgcctgacc agttttccgc ctttcacaaa gcgctggacc gcgcagcgaa
      481 cgacctgctt gccgatgcgg tccagcgcat ggccgacgcc gctgatgacg ggcagggagg
      541 tgcttcgcgg cactcgcccg aatcgctagt ccgtgacttc gaccagaaat atcgttcgga
      601 cctaaccccg agcgtgggtg cagcgatgaa gcgcctggat cagcttcgac cgacactgag
      661 tcgcatcttg gaaaccgagg gtgtcccacg ggagattgtc tccgtcgtcg tcgtcgagag
      721 cggcggcaga actaccgcgc tttcacctaa aggcgcactg ggactctggc agttgatgcc
      781 cgacaccgcc cgccgatatg gactggtcgt tactcccaca agggacgaga gactggacat
      841 cgagaaatcc accggtgcgg cggcgcacta tttgcgcgat ctctaccagc agtttggttc
      901 ctggcctctg gcccttgcgg cttacaacgc tggcgaacag gcgctccaaa gagcaatagc
      961 gcgtgccggc acacgcgatt tcgtgcaaat aagcagcctg cggctcctgc cccaagaaac
     1021 acggaactac gtgccggcag ttctctcagc gatgcagatg cttggcgtct catatttacc
     1081 ggttgagcca aggcagatcg caaagaaagg cgattccaac ggaatcatct tcgcggtggc
     1141 aggtgcacaa ccgtgaattc gtaataaatg ttctcgcgag gaggacccat gaaaaccgca
     1201 gtagttacaa tcctgatcgc agtcacactg attggaacag gtaccgcgga cggcgagtaa
     1261 tcaactgctg cggcctcgct ccctgtaaac catcccagtt ccgcgccgct tgagcgttcc
     1321 tataccttga ggtcacgtga atgtgcagaa atatcaaaat cctcttcaac ttcgatccgc
     1381 ccgtcactgc cgaagagatt cgagctgctt cgctccagtt cgtgagaaaa atcagcggtt
     1441 tcaacaaacc gtcgaaggcg aacgagagct cgttcctggc tgcagtcgat gaaatagccg
     1501 gcatctcgac tcgtcttctg cgttcgctcg aaacgaacgc accgccaaaa aatcgagaag
     1561 aagaggccgc aagagcgaaa gctcgtgccg cagacagatt cggtgcatga gtctgccaaa
     1621 cccgtctggg gctaggatta tggatggatg acggagactg aagttgtcgg cctggttcca
     1681 gtttcgtaaa gcagtttcac actcggtgta tagggtggag caccgcttca gcggtgcagt
     1741 taagattcgg aaatgagccg gctttagcca ctgaggtgac ggaaacatta cctcaggcgc
     1801 taaagcgcga tccaggaact aacggcagtt atgcacgcct gaaaggcttg ctccacccat
     1861 cgcctataca ccagatgtga aactgcttta gtcgccggcc atccatccgg aacctcgccc
     1921 cctcacagtg cagccgtgca atttcgccca gcactcttag cctggtacaa ggcagtatcg
     1981 gcgattgcaa gaatctcttt ttcagcgacg gtgctgaccg tcgtaaccgt caccccaatg
     2041 ctggccgtaa ccacgatttc ggcgccagct gccagaactg ggcgttcggc gattaccgat
     2101 cgaattctct ccgcgctttg cagggcttgg atcttgtcgc agctggggag cacaaccagg
     2161 aactcttcgc ctccatagcg ccccacgagg tcataggaac ggacaacttc gctgatgcgg
     2221 cgcgcaactt ccctcaggac cacatcgcca gccagatgtc cgtaggtgtc attgaccttc
     2281 ttaaagtggt ccagatcaag catcaaaaga ccggttgggc tttgggaacg gctcgctcgc
     2341 tcgatttcac gctgcagcag gtcgaggagt gcaccccggt tccaaattcc ggtaggtatg
     2401 tcgtgagtgg cctgaaagcg gagctcttcg cgggcttgaa taagaccttt ctggagagtg
     2461 aggatacgtc ttcccactct cagccgcgcc cgcagttcgc tgcggtcgaa cggcttggtc
     2521 aaatgatcat ccgctcctgc ctccaggccc gtgaccacat cctcggcatc gtccttggcc
     2581 gtgactagca ggatgtattg gtaaggagtt gtctggcgct ctcgaattct gcggcatagc
     2641 tccaagccat caattcccgg catcac
//
LOCUS       DASRKK010000097         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_885504, whole genome shotgun sequence.
ACCESSION   DASRKK010000097 DASRKK010000000
VERSION     DASRKK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885504"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFC15_02045"
     CDS             complement(<1..447)
                     /locus_tag="VFC15_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZM08972.1"
                     /translation="MAAPLNSADIQPRQVANKSNKKALLDAIRMTLDVQSAAIRLNTQ
                     TFNRNRYAATALIADYNELKDHARQIKEAAIANLPELLRRLEASVQANGGHFYLAKTA
                     ADANQYIRDVLVRHQVKRVVKGKSITSEEIKLNHAIEAAGIAVAETD"
     gene            complement(451..996)
                     /locus_tag="VFC15_02050"
     CDS             complement(451..996)
                     /locus_tag="VFC15_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZM08973.1"
                     /translation="MQQLMALESSVLQFAEAAVKAAATVEIIERSPAPLRDAILRAVG
                     DATRILFAPPHELPVALFSEFAADSRVVANPTPDEMVHSAAGVTEAFAGVASTGSVCV
                     DVGYERTGVVSLLAPLQIAVVAAETIVPKPRDLFRADILDGKGLKRDFVFVTGPSATA
                     DMGPLVRGVHGPHKLHIIVVR"
     gene            complement(1377..>2476)
                     /locus_tag="VFC15_02055"
     CDS             complement(1377..>2476)
                     /locus_tag="VFC15_02055"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZM08974.1"
                     /translation="IRVFDAYRVQHSNIRGPCRGGIRYHPSVTFDEVSALAAWMTWKC
                     ALVDIPFGGAKGGVGCDPKRLSRNELERLTRRYTYEIAPLIGPDQDIPAPDVNTDAQT
                     MSWIMDTYSMTTRHATPAVVTGKPTFLGGSLGRLEASARGCLFVLREASKVRGLDLKT
                     ATVAIQGFGNVGGVMARLLWDGGVRVIGVSDSKGGILNKEGIDIPALSRFKAATGSVE
                     AFPGCSPIPSDDVLKAQCDILIPAALENQITIENAGYVRAKIVAEAANGPTTPEADLM
                     LSRKEVMVLPDILANAGGVTVSYFEWVQDLQQLFWDEAEVNRRLEQAMVKTFHEVHDT
                     TKKYGCDYRAAAYIVAIGRVAKAVIERGIWP"
BASE COUNT          461 a          727 c          788 g          500 t
ORIGIN      
        1 gtcggtctcg gccaccgcga tgccggcagc ttcgatggcg tggttcagct tgatctcttc
       61 cgaggtaatg ctcttgccct tcaccacccg cttgacctgg tgccgcacca gcacgtcgcg
      121 aatgtactgg ttggcgtcgg cggcggtctt cgccaggtag aaatgtccac cattggcttg
      181 cacgcttgct tccagccgcc gcagaagttc gggaagattc gcgatcgcgg cctctttaat
      241 ctggcgggcg tggtctttca gttcgttgta atcggcgatc agcgcagtcg cggcgtagcg
      301 gttgcggttg aaagtctggg tgttgagccg gatggcggcg ctctgcacat ccagcgtcat
      361 gcggatggca tcgagcaggg cttttttgtt gctcttgttc gctacctgtc gcggttggat
      421 gtctgcggaa ttcaagggcg cggccatggc tcacctcacc acgatgatgt gcagtttgtg
      481 cggcccatgt acgccgcgca ctagcgggcc catgtccgcg gtggcgctgg ggccggtgac
      541 gaaaacgaag tcgcgtttca gccccttacc atccaggatg tcagcgcgaa acaggtcacg
      601 cggtttcggc acgatggtct cagccgcgac caccgcgatt tgtagcgggg ccagaaggct
      661 caccactccc gtgcgttcgt agccgacatc cacgcagacc gaacccgtgc tggcaactcc
      721 ggcgaaggct tcggtcacgc cagccgcgga gtggaccatc tcgtcgggag tcggattggc
      781 caccactcgg gaatccgccg cgaattcgct gaacagtgcg acgggaagtt catgcggagg
      841 agcaaagagg atacgcgtcg cgtcccccac agcgcgcagg atggcatcgc gcaagggagc
      901 tggagagcgc tcgatgattt cgacggtggc cgcggctttg acggctgcct cggcgaactg
      961 caggacggaa gattccaagg ccatgagctg ctgcataggg aacatcgcag cttataccga
     1021 atgatggcta aaagcacgga attcgttgga gcttctttgg aagaattcca cagtgattgt
     1081 agcgtgggca gtgcgggcct tcagttaaga accggtcact tgggcggagc cgcgaagcgg
     1141 cggggtgggc tccgttgaaa tacgtcccag gcgaaagtga gtgggcagta taggggtcct
     1201 tcgactcgct acgctcgctc aggatgacag acagaaaagc aggcccggtc gccacagtga
     1261 ccgcactcgt cgcttaagat gacagccgaa caaaggtttt catccctgtg gtgggccgca
     1321 gcccatgtga cactcgggtt ggcacctcaa gaaattgcat acgctcatga agaactctag
     1381 ggccagatgc cgcgctcgat gactgccttg gcgacacgcc cgatggcgac aatgtatgcc
     1441 gcagcgcgat agtcacagcc gtacttcttg gtagtgtcgt gaacctcgtg aaacgtcttc
     1501 accatcgcct gctccagcct gcggtttact tcggcctcgt cccagaagag ctgctgcagg
     1561 tcctgtaccc attcgaaata ggagacagtc acaccgccgg cattggcgag aatgtcgggc
     1621 agcaccatca cctctttacg gctgagcatg aggtcggctt ccggtgtcgt cgggccgttg
     1681 gcggcttcgg cgacgatctt ggcccggaca tagcccgcgt tctcaattgt gatctgattc
     1741 tccagggccg cgggaatcag aatgtcgcat tgcgccttca gcacgtcgtc gctgggaatc
     1801 gggctgcatc cgggaaacgc ctccacgctg ccagtcgctg ccttaaaacg cgacaaggcg
     1861 ggaatgtcga tcccctcctt gttaaggatg ccgcctttcg agtcgctgac gccaatcact
     1921 ctgacccctc cgtcccagag cagtcgcgcc atcacgccgc cgacgttgcc gaaaccttgg
     1981 atggcgacag tagcagtctt caggtcgagg cccctcactt tgctggcttc gcggaggacg
     2041 aagagacatc cgcgcgcact ggcttctaag cggccgagag aaccgccaag gaaggtcggc
     2101 ttgccggtga cgaccgcggg agtggcgtga cgtgtggtca tgctgtaggt gtccatgatc
     2161 cacgacatgg tctgcgcgtc ggtatttaca tcgggcgcgg ggatgtcctg gtcggggccg
     2221 atcagcggcg cgatctcata ggtatagcgg cgagtgaggc gctccagttc attccgcgac
     2281 aggcgcttgg gatcgcagcc gacaccgccc ttggcgccgc cgaagggtat gtccaccagg
     2341 gcgcacttcc acgtcatcca ggcagccaag gccgacactt cgtcgaaggt gacgctgggg
     2401 tgatagcgga ttccacctct gcacggtccg cggatgttgc tgtgttgcac gcggtaggca
     2461 tcgaagacgc ggatgg
//
LOCUS       DASRKK010000098         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_6120, whole genome shotgun sequence.
ACCESSION   DASRKK010000098 DASRKK010000000
VERSION     DASRKK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6120"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFC15_02060"
     CDS             complement(<1..1241)
                     /locus_tag="VFC15_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08975.1"
                     /translation="MHIAARSLGRVVVILTLAALIPASYAADRQRIQVENYVIQAVVS
                     PQIHQIKAIARVKFTALDDISIATFVLHNGLRPTRVLDADGQTLSAERISQDSTVRVS
                     LPNGLSKGASSTLTFEYEGTIQSADDSPVPGLKLAYIGDPITYLLYAGAWFPMVGYGT
                     NRFSSTISISAPTGYTVIGSGKDSTGAMPSLGIAESEAKAPRAGVKKGAAGSGPGKLS
                     IPEGNSTHTFTYEKLSFPGTILIGKYDQTKSSEGGLDITVYTTAANKPVAQAYAEAAG
                     KAFFFFTSTFGPAYSGKLNVVELPNDTVPSAWAPEIAAIAARAFNQKVNYRLLANTIA
                     HQWWGTMVSPATRNDWWIDDGFARYSEALYVLYIAGQAGFEEVTKDMAVGALAYISVP
                     LSQVGKLDVFSPEFQSLVTDKG"
     gene            complement(1313..2470)
                     /gene="lpxB"
                     /locus_tag="VFC15_02065"
     CDS             complement(1313..2470)
                     /gene="lpxB"
                     /locus_tag="VFC15_02065"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZM08976.1"
                     /translation="MRILISAGEASGELYGAKLIEALRQEGPQRSPQLALEFFGVGGE
                     RMQQAGCELVVNAHEIAVVGLAEVVEHLPRIYQRFRRVVAEAERRRPDAAVLIDFPDF
                     NFRLARELHTRGIPVIYYVSPQLWAWRQSRIELVRKYVRKMLVIFPFEEKFYRERGIE
                     AEFVGHPLADLPLPSITRDEFAAGYGIDAQRPWIALLPGSRKGEVSRIFPKLLEAAQL
                     LGPDYVYIVPVASTLDIHWMTSLLQSHTGPPITFTNDARATLRHARVAAVASGTSTLE
                     AALIGTPFAMVYQVSPLTWTFGRRLVKVDRFAMVNLIAERDVVPELVQDGFTAARVCA
                     ELQTVLADGSARDAMMQGFDEVRHRLRPSDTEGSASERAARAVLTVLGQAS"
     gene            2559..3014
                     /locus_tag="VFC15_02070"
     CDS             2559..3014
                     /locus_tag="VFC15_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZM08977.1"
                     /translation="MSAANSQPLSALVRALISGYVDSYALLNFGVFVSFMTGNTTAGG
                     LSAGQVNLAAAGHSLLPIPFFLLGILGGKFLVRVEQHHALRRISLLVAVVLGVAAARA
                     YFAWPGWLGILLLSSAMGLMNTSVTHVGGQPVSLGFMTGDLNNLAQHLA"
     gene            3093..3230
                     /locus_tag="VFC15_02075"
     CDS             3093..3230
                     /locus_tag="VFC15_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08978.1"
                     /translation="MWIFFLAGAVLGSALASRIAVWTLLLPAATLLLLAVLEPAATPA
                     D"
     gene            complement(3316..4245)
                     /locus_tag="VFC15_02080"
     CDS             complement(3316..4245)
                     /locus_tag="VFC15_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08979.1"
                     /translation="MNTQSNAIPDTPVEAQGFAPAVIPESRLLHWLVQRELWENRSIY
                     IAPLAVAGLILFGYLSNAIHTWFGLGIMSARQLTEREMLAQPYDFAALLIMGTTFIVG
                     VFYCLDALHGERRDRSILFWKSLPVSDLMTVLSKASIPLVVLPLLTFAITVVTQWIML
                     LMSSAVFLAKGQSVTTLWSQLSLSDSWLGLLYHLVTVHALWYAPIFCWLLLVSAWARR
                     AAILWAAIPVLAIALVEKIAFNTSYFGAMLANRMGGGAEAVVAPGGVPMDPTMTHLTP
                     VHFLSSAGLWIGLLIAAAFLAGAVRLRRYQGPI"
     gene            complement(4242..5117)
                     /locus_tag="VFC15_02085"
     CDS             complement(4242..5117)
                     /locus_tag="VFC15_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM08980.1"
                     /translation="MACIEARGLRKAFGTTIALDGINMRVEEGRILGLIGPNGAGKTT
                     ALDAILGLTPYQGQLRTLGRDPWNERDQLMRDVCFIADVAVLPRWARVSQLLDYVGGV
                     HPRFNRAKAEGFLAKTTINRTSKVRELSKGMVTQLHLALVMAIDARLLVLDEPTLGLD
                     ILYRKQFYDSLLNDYFDHNRTIVVATHQVEEIEHVLTDVMFIDRGRIVFECSMDDLES
                     RYAEVKVRPEQVAAARALRPMYEHQAIGGSILLFDGVDRQQLAALGEVRTPGIADLFV
                     AVIGSQPGRAQGAAR"
     gene            complement(5122..5544)
                     /locus_tag="VFC15_02090"
     CDS             complement(5122..5544)
                     /locus_tag="VFC15_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZM08981.1"
                     /translation="MDREWSDSQPIYRQLRDLIVAMILDGVLKDGDPLPIVRNVAAEF
                     RVNHLTVLKSYQKLVDDGLVESRRGRGMFVNPGARKLLLKLEREKFLGEEWPRVYETI
                     LRLGLNAEDLLNNKPHNSPEEPAEPEATESNPRKNEEG"
     gene            complement(5596..6165)
                     /locus_tag="VFC15_02095"
     CDS             complement(5596..6165)
                     /locus_tag="VFC15_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08982.1"
                     /translation="MKHPRLLLSVVLIALSTTMVFAQSDAQKASVASVPSAAQQSFTT
                     IKTLAGEWEGPITVPEMPDMPGGKLMHASLRVTSRGHVLVHELQEAGTPLDATKYDHR
                     VTMLYVDGDQLTLVHYCDAGNRPRMVGKMSPDGKTIEFEFKDISGSTEYHMHNSVFTI
                     IDANHHTEDWTFMMKDKPIHAHFDLHKVN"
BASE COUNT         1379 a         2183 c         1878 g         1427 t
ORIGIN      
        1 cccttgtccg tcaccagcga ctggaactcc ggcgagaaca catccagctt gcccacctgc
       61 gacagcggaa cactgatgta ggccagcgct ccgaccgcca tgtccttggt cacttcctcg
      121 aacccagcct gccccgcgat gtacagcacg tacagcgctt ccgagtaccg cgcgaagccg
      181 tcgtcgatcc accagtcgtt gcgggtcgcc ggactgacca tcgtccccca ccactgatgc
      241 gcgatggtgt tggccagcag gcgatagttc accttttgat tgaaggcccg cgcggcgatc
      301 gcggcgattt ccggagccca tgccgaaggg actgtgtcat tcggaagttc caccacattc
      361 aatttgccgc tgtaagccgg tccaaacgtt gacgtgaaaa agaagaacgc tttccccgcc
      421 gcttcggcat acgcctgcgc taccggttta ttcgcggcgg tagtgtaaac cgtaatgtcg
      481 agcccgccct cgctactctt tgtctggtcg tacttgccga tcaggatggt tcccggaaaa
      541 ctcagtttct catacgtaaa ggtgtgggtt gagtttccct caggaatcga aagctttccc
      601 gggccggagc ccgctgcgcc tttcttaacg cccgcccgcg gcgccttcgc ctcgctttca
      661 gctattccca gcgacggcat cgccccagtt gaatccttgc cgcttccgat cacggtgtag
      721 cccgtcggag cgctgatgct gatggtcgaa ctgaagcggt tcgttccgta tccgaccatg
      781 ggaaaccatg cgccggcata tagcaggtac gtgatcggat cgccaatata agcgagcttc
      841 agtcccggaa ccggactgtc gtcggccgac tgaatcgtcc cctcatattc aaacgtcagg
      901 gtcgaacttg cgcctttact caggccgttc ggaagcgaca cacgcaccgt cgaatcctgg
      961 ctaatgcgtt ccgccgacag cgtttggccg tccgcatcga gcacccgcgt cgggcgcagt
     1021 ccgttgtgca gcacgaaagt cgcaatactg atgtcatcga gcgcggtgaa ttttacccgc
     1081 gcaattgcct tgatctgatg gatctgggga gagactaccg cctggataac gtaattctca
     1141 acctgaatgc gctgccggtc ggcagcatac gaggccggta tgagagccgc cagagtgagg
     1201 atgactacca cgcgtcctaa ggatcgcgct gcgatgtgca ttatggagag aactcctgcc
     1261 cttctgacta taaatgatgg aaaaactact tggaaggatg cgtgccgggc cttcacgatg
     1321 cctgtcccaa aacggtcagc acagcccgcg ccgcgcgctc gctggcgctg ccctccgtgt
     1381 ccgacggacg caaccgatgt cgcacttcgt cgaatccctg catcatcgca tcgcgcgcgc
     1441 tgccgtcagc caacactgtc tgcaactcgg cacagacccg cgccgcagtg aatccgtcct
     1501 gcaccagctc gggcaccaca tcgcgctcgg caatcaagtt caccatcgca aaccggtcca
     1561 cctttactag acggcgtccg aacgtccagg taagcggaga cacctgatac accatcgcga
     1621 acggcgtgcc gatcagcgcg gcttccagcg tcgacgttcc gctggctacc gcggccacgc
     1681 gagcgtgacg caacgtggcc cgggcatcgt tagtaaacgt aattggcgga cccgtgtggc
     1741 tttgcagaag agacgtcatc cagtggatat cgagcgttga ggctactggc actatataaa
     1801 cgtagtccgg ccctagaagt tgcgctgctt ctaacagctt gggaaagatg cgcgacacct
     1861 cgcccttgcg gcttcccggc aacagcgcaa tccacggacg ctgtgcatcg atgccgtacc
     1921 cagcggcaaa ctcgtcgcgc gtgattgacg gcagcggcaa gtccgccaac ggatgcccca
     1981 caaactccgc ttcgatgccg cgctcgcgat aaaacttctc ctcaaacgga aaaatcacca
     2041 gcatcttgcg gacgtacttc cgcaccagct cgatgcgcga ttgccgccac gcccacaact
     2101 gtgggctgac gtaatagatc accgggatgc cgcgcgtgtg cagctcccgc gccaaccgaa
     2161 aattgaagtc cgggaagtcg atcagcacag cggcatccgg acggcgtcgc tctgcctcgg
     2221 ccaccacccg gcgaaagcgt tgatagatcc tcggcaaatg ttccacgacc tccgccagtc
     2281 ccaccaccgc gatctcgtgc gcgttgacca cgagctcgca tcccgcctgc tgcatgcgtt
     2341 cgccacccac gccgaagaat tccagcgcaa gttgcggaga tcgctggggg ccttcctgac
     2401 gaagggcctc gatcagcttc gccccataaa gctcgccaga agcctctccc gcggagatta
     2461 ggattcgcac ctaaggatta tgacattcgg gcgattagcc gcccttcatc gcccttatag
     2521 tattgtcgaa caccgtgcga tcccgtcaag gaggctcgat gtcagccgcc aactcccaac
     2581 cgctttcggc cctcgtccgc gccctcatct ccggctacgt cgattcctac gcgctgttga
     2641 acttcggagt cttcgtctcg ttcatgaccg gcaacacaac cgccggcggc ctcagtgccg
     2701 gacaagtcaa tctcgccgcc gcaggacaca gtcttctccc cattccgttt ttcttgctcg
     2761 gcattctggg cggcaagttc ctggtgcgcg tagaacaaca tcacgcgttg cgcaggattt
     2821 ctttgctggt cgccgtcgtg cttggcgtcg ccgccgcgag ggcttacttc gcttggccgg
     2881 gatggctcgg cattctgcta ctcagcagcg ccatgggcct catgaacacc tccgtcaccc
     2941 acgtaggagg tcagcctgta agcctgggct tcatgaccgg agacttaaac aatctcgccc
     3001 aacacctcgc ctagggaatc ctgtgcgcgc ccgtcgccca ggcccaaggt gctcgcgaca
     3061 cccattggcg gcgcgctgcc ctgctgggta gtttgtggat attttttctt gccggtgcgg
     3121 tcctcggcag cgcactcgcc tcgcgcatcg cggtgtggac cctgctgctg ccggccgcga
     3181 cgttgctgct cctcgccgtg ctggagcccg ccgcgactcc tgcggattag agtcgcgaaa
     3241 ctgcccattg gttcgcggcg tagctcgcct gcgccaggtc cttacgctgg aattggaacc
     3301 aatgatggat acaactcaga tcggtccctg ataccgccgc agcctgaccg ccccagcgag
     3361 gaatgccgcg gcaatcaaca gcccgatcca cagtcctgcg ctgctcaaga aatggaccgg
     3421 agtgaggtgc gtcatagtcg gatccatcgg gacgccaccc ggcgctacga ccgcttccgc
     3481 accaccgccc atgcggttcg ccagcatggc gccgaaatac gaagtgttaa acgcgatctt
     3541 ctccaccagc gcgatcgcca gcaccggtat cgcggcccat agaattgccg cgcgccgcgc
     3601 ccaggctgag accagcagca gccagcagaa gatcggcgca taccacagcg catgcaccgt
     3661 caccaggtgg tagagcaatc ccagccacga gtcggacaac gacagttggc tccatagcgt
     3721 tgtgacgctt tgacctttcg ccagaaaaac tgcgctgctc atcagcagca tgatccattg
     3781 cgtgaccacg gtgatcgcaa aggtgagcag cggcagaacc accagcggaa tgctcgcctt
     3841 cgagagcacg gtcatgaggt cagagaccgg cagcgacttc cagaacagga tactgcgatc
     3901 gcgacgttcg ccgtgcaacg cgtcgagaca gtagaacacc ccgacgataa aggtggttcc
     3961 catgatcaga agcgcagcga agtcgtacgg ctgcgccagc atctcgcgct ccgttagttg
     4021 gcgcgccgac atgatgccca atccgaacca cgtatgaatc gcattgctca agtagccgaa
     4081 caaaatcagg cccgcaaccg ccagcggcgc gatatagatc gagcggttct cccacaattc
     4141 gcgttgcacc agccagtgca ggagacggct ctccgggatg accgcaggcg cgaacccctg
     4201 cgcttcgacg ggagtatcag gtatggcgtt ggactgagta ttcatcgcgc cgctccttgt
     4261 gcccggccgg gctgactgcc aatcactgca acgaacaagt cggcaatgcc gggagtgcgc
     4321 acctcgccaa gcgctgcgag ttgctgacgg tccaccccgt cgaacagcag gatgctgccc
     4381 ccgattgcct ggtgctcata cattggcctg agcgctcgtg ctgccgcaac ctgctcggga
     4441 cgcaccttca cttccgcata gcgcgattcc agatcatcca tgctgcactc gaacacgatg
     4501 cgtccgcggt cgatgaacat cacatcggtg agcacgtgct cgatctcttc cacctggtgt
     4561 gtcgctacca cgatggtgcg gttgtgatcg aagtagtcat tcagcaggga atcgtagaac
     4621 tgcttgcgat acaggatgtc cagtcccagc gtcggctcgt ccagcaccag cagtctcgcg
     4681 tcgatggcca tcaccaacgc taggtggagc tgtgtcacca tgcccttcga caactctctg
     4741 accttactgg tgcgattgat ggtggtcttc gctagaaaac cttccgcctt agcacgattg
     4801 aatcgcggat gcactccgcc gacgtagtcg agcagctgcg aaacccgcgc ccagcgcggc
     4861 agcacggcga catcggcaat gaaacaaaca tcgcgcataa gctgatcgcg ctcgttccac
     4921 gggtcgcgtc ccagcgtcct cagttgtcct tggtaaggcg tgaggccgag gatcgcgtcc
     4981 agtgcggtgg tcttacccgc gccgttggga ccgatcaacc ccaggatgcg gccctcttcc
     5041 acccgcatat tgataccgtc cagcgcgatg gtcgtgccga aggccttgcg aaggcctcga
     5101 gcctctatac atgccatggc ttcaaccctc ctcattcttg cggggattcg attcggtggc
     5161 ctccggctct gctggttcct caggcgagtt atgtggttta ttgttcagca ggtcttccgc
     5221 gttcaagccg agccgcaaaa tcgtctcgta caccctcggc cattcttccc ctagaaactt
     5281 ttcccgttcc agcttcagca gcagtttccg tgcgcctggg ttgacgaaca tgccgcggcc
     5341 tcgcctcgac tccaccaggc cgtcgtccac cagtttctga tagctcttca ggaccgtgag
     5401 gtggttgacc cgaaattcag ccgcgacgtt gcgcacgatg ggcagcggat cgccgtcctt
     5461 cagaacgcca tcgaggatca tcgcaacgat aaggtcgcga agctggcggt aaatcggctg
     5521 actgtcactc cactcacggt ccatcagttt ttccagataa cctttaccca gaccttacca
     5581 gagataccgc gcgcgctagt tcaccttatg caggtcgaag tgcgcgtgga ttggcttgtc
     5641 cttcatcatg aacgtccagt cctcggtgtg atggttggcg tcgatgatgg tgaacaccga
     5701 gttgtgcatg tggtactccg tgctcccgct gatgtccttg aactcgaact cgatcgtctt
     5761 gccgtccggc gacatcttgc ctaccatgcg aggccggttc cccgcgtcac aatagtgaac
     5821 cagcgtgagc tggtctccgt ccacgtaaag catcgtgacc cgatggtcgt actttgtcgc
     5881 atccaacggc gttcccgctt cctgcaactc gtgcactagg acgtgtcccc gcgacgtcac
     5941 gcgcagcgaa gcatgcatca gcttgccgcc tggcatgtcc ggcatctccg ggacggtgat
     6001 cgggccttcc cattctcccg ccagggtttt tatggttgtg aaggactgct gtgccgcgga
     6061 cggcaccgaa gcaacggatg ctttttgcgc gtcggactgc gcgaaaacca tggtagtgga
     6121 gagcgctatc agaacaaccg acagcagcaa acgagggtgt ttcatgttca ttctcctcac
     6181 gaagctcatt tcgtcttttg cggtcgggct gagaggcctg caatcgagca gctgaaataa
     6241 acttcaaact agttgtatac ttagctatat aactatatat ctagtcaagt cttttctagc
     6301 ttttctttgc agaaaagcca gcccgagggt tctgtttcat tccgggaacc gtgattacct
     6361 tcgtaatgtc gccatgtcga tttcccttgg ctcgacttac tggcattctt tatcaagggg
     6421 ttagcaggtt ctgcgctctg aataccagcg cgaaactaga cgctcgtggt gggcgacggg
     6481 tgcaggtttg cgattgtcga gaacgacgcg gtgcccacaa ttgcctgccg tttgctaatt
     6541 gggggcccct gaatcggccc aagcgaagca tcgccagccc gcccagtgcg gtctgccgtg
     6601 ccagctaagc tcttggaata gaagattttg cctgtaagtc acacaaacga cgtattttgc
     6661 gcgagaattc ttcctaaatc catgattcga cggatctttg acaagtgaaa actagagcgt
     6721 gttaatacta aacaggaatc ggagtgcttc cacgatcagc gcgaagtgga tgaagcccac
     6781 gaagatcaca tcgagtttct cgaagcgcga gaagatgcga cggaagccct tcagtcgacg
     6841 aaacatcctc tcgacctcat tgcgttt
//
LOCUS       DASRKK010000099        16359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_6138, whole genome shotgun sequence.
ACCESSION   DASRKK010000099 DASRKK010000000
VERSION     DASRKK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16359
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6138"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..335)
                     /locus_tag="VFC15_02100"
     CDS             complement(63..335)
                     /locus_tag="VFC15_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08983.1"
                     /translation="MVKTLLEDVREHIAESSKHIDQAKGVVNDACDDFVRESKRAVKA
                     GRGAAEDFLDQAEHSVKKYPKAAVASGVMTGMLLGFCLGWLLARKD"
     gene            complement(502..1179)
                     /locus_tag="VFC15_02105"
     CDS             complement(502..1179)
                     /locus_tag="VFC15_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HZM08984.1"
                     /translation="MFRIITVEREYGAGGSMVAAELARRKVWQLVDQEMTAEIAKLAS
                     VDSRVVARCEEKCDTLMHRLGKVFWRGSYERSLPIADDKIFDADSMVQLAHKVIEDKA
                     KHGHCVIVGRGAPYILRNRHDTFRVFVFGSREEKIRRLLRLKMQEKEAAELVDTIDQE
                     RALFVRKYFNAEWPCRRLYHMMLNSDAGIEFVAETILQAMDRIEAAQNVARGTVHGDE
                     TLHPHPA"
     gene            1515..2090
                     /locus_tag="VFC15_02110"
     CDS             1515..2090
                     /locus_tag="VFC15_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZM08985.1"
                     /translation="MGRKMMITGLLFVVALTASAQISSNGPVVKDYPQPVSGENPAEA
                     KIAKEVRHEILMLPWYSLFDDLEFTVQGRTVTLSGFVTSEHAGTKSDAANVVKRIEGV
                     DNVVNNIKVLPPSPMDTEAREQVYAALSRTGGLSRYFWEAAPSIHIIVDNQRVTLAGF
                     VNNEGDKNLATLAANGVPNVFQVTNNLRVVK"
     gene            complement(2310..2861)
                     /locus_tag="VFC15_02115"
     CDS             complement(2310..2861)
                     /locus_tag="VFC15_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZM08986.1"
                     /translation="MPDCVLKFSLILATVGRTEDVANFLAHIASQSYRSFELIVVDQN
                     QDDRLVAVLEKYAPQFPIQHCKSAIGLSRARNVGLERATGNVIVFPDDDCWYSDDLLG
                     RVARILVANPELDGVTGRPIDSSFSRFHRTSGPIKYFSLGCGSFSVRVEANGAGGGRE
                     ASPVSIVCWAATRKHNRVLKIVK"
     gene            complement(2905..4341)
                     /gene="wzy"
                     /locus_tag="VFC15_02120"
     CDS             complement(2905..4341)
                     /gene="wzy"
                     /locus_tag="VFC15_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HZM08987.1"
                     /translation="MSSAAPAYPLPSYGALRIPAAAFEILLYLAVVDIATLCFLAGWL
                     PVNGAVVLTVVLLTSLIVMSWVNLGQGRHPCFLFLCTLMLFQGGRLVGYCLGSISDPF
                     MVELMVAEPFGISRIEAGVVMLCIALSAICIYGPCRWNYRRIPPPTDVNVRQDLPYLY
                     LVFFSSLPFLLYKNYLYFRYIQEHGGYIVFFSDYGHLAASVPLVVRAVALLTLPVLVA
                     IVVFEKRKKRLYTVVALYFCSSVVLLLTGTRMGPFSLILTIWYVARIKSTKDTRIWRM
                     VVLVAALVVVANLIGLARFGEDVKGRSAIDPVGFTAAQGASLAVTEVAVVHGNLFKPY
                     VVSYLLHELEIEFVASDVSTYFRGRQFGFDVSVFLNPSMFDQGFATSGAYIAEAYVIG
                     GALGVIVISLLIGGGLHFMYTSSRNAAMLLLVALVFPEVLLLPRGFLLGWVSALIRAF
                     ALLVPLALGWCLFQLVTSVVKAPLTEWE"
     gene            complement(4338..5234)
                     /gene="rfbA"
                     /locus_tag="VFC15_02125"
     CDS             complement(4338..5234)
                     /gene="rfbA"
                     /locus_tag="VFC15_02125"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZM08988.1"
                     /translation="MNHNAPLKGIILAGGSGTRLYPATQCVSKQLLPVYDKPMIYYPL
                     SVLMLAGIREILVISTPLDTSRFEQLLGDGSRWGVNFSYVVQAAPEGIAQAFLLGADF
                     IASQRCALVLGDNIFYGHNLSGTLRVAASREQGATVFAYAVTDPERYGVVEMDANGRA
                     ISLEEKPKQPKSRYAVTGLYFYDRQVVELTRSLKPSARNELEITDLNRLYLDRGELHA
                     EIMSRGMAWLDTGTHDSLLEAATFIQTIEKRQGLKIACPEEIAYRAGYIDAAQIERLA
                     ATMSNNGYGQYLKGLLDTPGGL"
     gene            complement(5231..6331)
                     /gene="rfbB"
                     /locus_tag="VFC15_02130"
     CDS             complement(5231..6331)
                     /gene="rfbB"
                     /locus_tag="VFC15_02130"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZM08989.1"
                     /translation="MTDSIIVTGGAGFIGSNFILQWLAQESTAVVNLDCLTYAGNAAN
                     LASLGGNPRYQFVHGDINDRALVSRVFRDHHPSAIVHFAAESHVDRSILGPDEFIRTN
                     INGTFALLTEAKQYWGNLGAEDKKRFRFLHVSTDEVYGSLSETDAPFCETTGYAPNSP
                     YAASKAASDHLVRAYFHTYGLPVLTTNCSNNYGPFQFPEKLIPLVILNALKGETLPIY
                     GDGKNVRDWLFVGDHCAAILRVLHDGRPGETYNIGGRSEQRNIDVVRTICAILDELCP
                     DSQFKPHENLIRFVTDRPGHDRRYAIDPRKIEHELQWQPQETFESGIRKTVAWYLNNQ
                     PWVQDVTSGSYRDWIDTQYAQRSAAGASEVKR"
     gene            complement(6339..6944)
                     /locus_tag="VFC15_02135"
     CDS             complement(6339..6944)
                     /locus_tag="VFC15_02135"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZM08990.1"
                     /translation="MALKVLQEKTGLIQPDAQARALIGAVADFRAQVAAKQVQLQSLR
                     TYATEDNPDVKRVERELAELQAQSAKLSQMERSGADLGEGNLQVPTRRVPEANLEYIR
                     VARELKYHESLYDFLGKQLEAARIDESKNAVMVQVVDKAVPAERKSSPKRLLIIAVTA
                     IATFLLSCLGVLIWEAIRRKQQDPNEAARLAQLQNYLRNST"
     gene            7491..8267
                     /locus_tag="VFC15_02140"
     CDS             7491..8267
                     /locus_tag="VFC15_02140"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HZM08991.1"
                     /translation="MPGGAQSQLLECSDGCHYVVKFQNNPQHPRVLVNDWLGTRLAAS
                     IGLPVPTVAVVHVDQQLIDSSPGLILETRGRHSVIAPGLCFGSRYVVSPLEGQVYDYL
                     PESMMVHVRNLSDFAGILGLDKWTCNCDGRQSVFWKRNREHRFTASFIDQGFCFNSGE
                     WNFPDAPLRGVFGRNDVYRSVTSWDCFEPWLSRVENLPESLVWSLAQEIPPEWYGSAT
                     EELERLLSQLLTRRARVRQLILDFKNSSRNPFPNWREIEN"
     gene            complement(8315..9016)
                     /locus_tag="VFC15_02145"
     CDS             complement(8315..9016)
                     /locus_tag="VFC15_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08992.1"
                     /translation="MIDHSDGLISVSPWLQMFSDAKDFHGTTRKKVALQCGSRYLVEP
                     RDGKLFDYFPETALSRVGNLNVFAGALAFDLWMKRAGARQTVFCRSSHDTLYNAAFLY
                     HGWEPRKCDPIGCAFDPARVAMYRHKAVYQDISEWCDFEPFLSRVGAVNSRILEGIAG
                     QIPAIWCDGCNWVSPFLRELRKRQEIVRPLLSRFLFANPDIFPNWSDTELVANVRDRE
                     SVRYEIDNMSELAVM"
     gene            9461..9946
                     /locus_tag="VFC15_02150"
     CDS             9461..9946
                     /locus_tag="VFC15_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM08993.1"
                     /translation="MTVNGNLTTVMCDDWAHGGAPGDTWQANFTNLGTSNLSLVRFNQ
                     LPSALTLYDEAGWLLLQTRVTPQAQWKDINYAVWHIFDPNAPLPGTAPLYWVNLAQQE
                     ATLGFPGVDFYQVGIFTPLNQHDTNLNGPQEFLNIVPEPSTLLLLGTGLAGLLGRKWL
                     T"
     gene            10466..11809
                     /locus_tag="VFC15_02155"
     CDS             10466..11809
                     /locus_tag="VFC15_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08994.1"
                     /translation="MSYALGGVQYKYHTDKLSPFLRTFVGAASISPDCCGGNQWNLAA
                     GGGGGLDLAVSPRFSIRLAQVDYIYSNYRHRFVTDHATQWNSVRLAAGVVFNVGSYCN
                     IMPLSCSVAASPTPAEVFAGEPIRLNTAGTNFNSKHAVNYSWAATGGRLSSPTTQATE
                     IDTTGLAPGSYSVNATMTDPTIKKVNSATCATSFIVKQPPASVPPLVSCSVDPASIAV
                     GGSATVTLAASSPDRRPLTYSWSSTGGQLSEQGASAILTVYAADAGKTIAVTGTATDD
                     RALSMSSTCNVDVKAPLTPPCVKIEDWGECTFEKDPKRPWRVDNDCKDVLDKLALRLR
                     EMPTGNLAIVGYAEEKEVVSNQTLGVQRSVNVKRYLTTDGPTKSDAARIQPRQGGTKD
                     AVTHFYFIPEGKLCAGELESFGSLVDETKVTGSSRDVPPRGNKAATPKPPVSPSH"
     gene            complement(11981..12056)
                     /locus_tag="VFC15_02160"
     tRNA            complement(11981..12056)
                     /locus_tag="VFC15_02160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12021..12023),aa:Ala,seq:cgc)
     gene            complement(12157..13251)
                     /locus_tag="VFC15_02165"
     CDS             complement(12157..13251)
                     /locus_tag="VFC15_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM08995.1"
                     /translation="MHPRTHMRSPYMEFAKLRSGAKYNLATSGIMSYPLSELPVRLTD
                     LEINGPGVYGYAPLQERLARYNGVPPECVFAAAGTSMANHMAMAATFNPGDEVLIEHP
                     TYELLESTAVYLGAQIRRFERRLEDGFRIDPSEVERQITPATKLIILTNLHNPTGAYA
                     EEATVRAVGEIAKSHGARVLVDEVYLEEFYEERPRPAFLLGDQFVVTSSLTKAFGLSG
                     LRCGWVLASQEITARMWHINDLYAATPAHPAELLSVIALDHLDKVATRAKNLLAENRR
                     SLNAFLDSRTDLDFYRPGEGTIVFPKLRSGDVDGFVVLLREKYETSVVPGIYFDMPQH
                     FRIGIGGDPEMTRMGLERLGQALDEYGRKA"
     gene            complement(13261..14646)
                     /locus_tag="VFC15_02170"
     CDS             complement(13261..14646)
                     /locus_tag="VFC15_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM08996.1"
                     /translation="MMTSEPTEATPSQSVAPVQLLRQIGLFDATMIVMGGIVGAGIFI
                     NPYVVARQVHTPMLILGVWVAGGVVAMLGAFIYAELAARMPDVGGQYAYLREAFHPIV
                     GFLYGWVLLLVIQTGGMAAVTVTFARYLLELTNWPVSERMVAVTTILLLTTINCLGVK
                     AGSRVQSVLMLLKIGAIATLVLAGLFLIRAPYPMLHPVLDRKPSIDLATAIGAAMVPV
                     LFAFGGWQTANFIATEIKHPQRNLSRALVMGVTGVIVLYLGVNFVCVRALGAEGLAAS
                     TVPASAVMHLAMGNTGARLIALGIAISTLGFLSQSILTAPRVYFAMASDGLFFKAVAR
                     VNKKSHVPVLAIVLQSVLTLVIALSGSYEKILNYVVSMDFIFFGLTGAALFVFRLREA
                     KLTSLPDTAMGSYRVPGHPYTTGLFVAICWLVVANTIYKYPANTLLGMGILALGLPVY
                     AFWARRKKGLE"
     gene            complement(14706..15671)
                     /locus_tag="VFC15_02175"
     CDS             complement(14706..15671)
                     /locus_tag="VFC15_02175"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZM08997.1"
                     /translation="MTSIEQFGVSENEIKHGAELLRGGKLVAFPTETVYGLGANALDA
                     IAVARIFEVKGRPNTSPVIVHVSSFEMIGRVALSWSEKARILAERFWPGPLTLVVPKT
                     TEVPDRVTSGLPTVGVRMPAHPVALALIEAAQVPVAAPSANRFTQISPTTAEHVRLGL
                     GELVAYILDGGPCEVGIESTVLSLVDEDPVLLRPGGISRRQIEELIGAIAQSEHEPTG
                     AHPSPGMHPRHYSPQTPLYLVHGGQVPLQGAGAYLQIQYPPVASVREVVAMPNNAADY
                     AARLYTVLHDLDAHGYDWIAVEITGPGVEWEGVLDRLRRAATSGA"
     gene            complement(15695..>16359)
                     /locus_tag="VFC15_02180"
     CDS             complement(15695..>16359)
                     /locus_tag="VFC15_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HZM08998.1"
                     /translation="PPGAARNTWVGKRTSLHALGYGFLLLFNGREYQELKASGDAPGV
                     GFRDAAAAVKAATSEGFPKGAIIFLDQEQGGRMLPEQRAYIHAWIDGVVKGGYGAGVY
                     CSGIAFRESSKVSVVTADDIHDNAAGRNIHFFISNDQCGPAPGCVFPDAPPAPAKSGV
                     TFAKIWQYAQSPRRPEMTAACRQTYAPDNNCYPPGIAPNAGLHIDIDSADSADPSHGR
                     SQ"
BASE COUNT         3585 a         4791 c         4546 g         3437 t
ORIGIN      
        1 gaggctctgc cgccacggca gcgaccggtg gcgtgttttg cgttccgcgc gattgctgaa
       61 gcctaatcct tacgcgccag caaccatccc aggcaaaatc ccaagagcat cccggtcatg
      121 actccgctcg caaccgctgc cttcggatat ttcttgaccg agtgctcggc ctgatccagg
      181 aaatcctcag cagctccacg cccggctttg accgcgcgct tcgactcccg aacgaagtcg
      241 tcgcaagcgt cgttcacaac gccctttgcc tgatcgatgt gtttgctgga ttccgcgata
      301 tgttcgcgga cgtcttcgag tagggttttg accattcggg ctacctcctt gcggttcggc
      361 gacccttctt tggcctcagt ctagtcctgg cgaaagggat tgcaagtctg tacgtcacat
      421 cgcaggtgtg acaacgcata acactggatg gtagtggaga tacggtatgg cgcgtgagcg
      481 aattgcggtt ttcttgtcca ctcacgccgg gtggggatgc agtgtctcat caccatggac
      541 agtaccgcgg gcgacgttct gcgcagcctc aatcctgtcc atcgcctgca gaatcgtctc
      601 cgccacaaat tcgatgccag cgtctgaatt gagcatcatg tggtagaggc ggcggcacgg
      661 ccactccgca ttaaaatact tacggacgaa cagcgcgcgt tcctggtcga tggtatccac
      721 caactcggcc gcttctttct cctgcatctt caggcgtaac agccggcgga ttttctcctc
      781 tcgcgagccg aacacaaaca cacgaaacgt gtcgtggcga ttgcgcagga tgtacggagc
      841 gcctcgtccc acgattacac aatggccgtg cttggccttg tcttcgatca ctttgtgagc
      901 gagctggacc atgctgtcgg catcgaaaat cttgtcatcc gcaatgggaa gcgaccgttc
      961 gtagctgccg cgccagaaaa ccttgccgag gcgatgcatc agcgtgtcgc atttctcttc
     1021 gcatcgcgcc accacgcggg aatcgaccga ggcgagctta gcaatttcgg ctgtcatctc
     1081 ctggtcaacc aactgccaga cttttctgcg cgccagctcg gccgcaacca tgctgcctcc
     1141 ggcaccgtat tcccgctcca ccgtgataat tctgaacatg cccgctctcc cttgctcgct
     1201 ttcgcctctg ttgcgctgtt gtatcccgaa agagtctgtc ttggcaagcc agttcggaga
     1261 ttccagttcg gagattccgt aacgtacgcc gggcctcctg ccggttacgc gagaggcggg
     1321 tctctcaatc cagattgcca tgcgtcgcat tttcattgac tcgcaagcct gcgaagtcag
     1381 tcagtttttt agggtaatca cagtacgctg taaccaattg tgtttgcaat gggttgcagc
     1441 gctctggcaa ttgcgaaaca cgtaactggc cggccttctg aagcatctaa acagcaggta
     1501 caggaggatc gtaaatgggc aggaaaatga tgataacagg gttgcttttt gtcgtggcct
     1561 tgacggccag cgcacaaata tccagcaatg gtcccgtagt gaaagactac ccccaaccag
     1621 tgtcggggga aaatccggcg gaggcgaaaa tcgccaaaga agtgcgtcat gagattctga
     1681 tgctgccctg gtattcactg ttcgacgatt tggaattcac ggttcagggt cgcaccgtca
     1741 ccttgagtgg tttcgtaacc agcgagcacg ccgggaccaa gtcggatgcc gcgaacgtgg
     1801 tcaagcgtat cgagggagtg gacaacgtgg tcaacaacat caaggtcctt ccaccgtcgc
     1861 ccatggatac ggaggcgcga gagcaggtgt atgcggctct ttcccgtacc ggaggacttt
     1921 cccgttattt ctgggaggca gcgccgagta tccacattat tgtggacaac cagcgcgtaa
     1981 ccctcgcggg ctttgtgaat aacgaaggcg ataagaacct ggcgacactc gccgccaacg
     2041 gcgttccgaa cgtttttcag gtgacgaaca atctgagagt ggtcaagtaa tctgaagcga
     2101 gtggagcaag actctacgcg cagtcgtttt tcgcagatca gcggcggaca atccgccgca
     2161 ttttgttttt aggcttctcc accgccgctg agttacttac ggacgatccg ttcggcgatt
     2221 cagtagaatc gtaaagccca tacttcataa ggcgcaggga gcgccaggtg tgggcttgtc
     2281 gtgcaggggt cattcttgaa ggctgcggtt tacttgacaa tcttgagcac tctgttgtgc
     2341 ttgcgcgtcg cagcccagca aacgattgat accggcgacg cttcccttcc gccgcccgct
     2401 ccattcgctt ccaccctcac tgaaaagctc ccgcatccca acgagaagta ttttatcggg
     2461 ccgctggtcc tatggaagcg ggagaacgag ctatcaatcg gccgtcccgt gaccccatct
     2521 aattcgggat tcgcgaccag gatacgtgcc acccgcccca gcagatcatc ggagtaccag
     2581 cagtcgtcgt cgggaaacac aatcacattg cccgtcgctc gctcgagacc aacgttgcgg
     2641 gcgcgtgaca ggcctattgc tgacttgcaa tgctggatgg ggaattgcgg cgcatatttc
     2701 tcaagcactg ccacaagcct gtcatcttgg ttttggtcaa ctacgatcaa ttcgaaagac
     2761 cggtaggatt gcgaggctat gtgggcaagg aagttggcaa cgtcctcggt tcggccgaca
     2821 gtcgcgagaa tcaacgaaaa cttaaggacg caatcgggca agtagactct cttccggcac
     2881 gggtatccgc tcgactgttt cgccttattc ccactcggtc aacggggcct taacgacgga
     2941 agtcacgagt tgaaacaaac accacccgag ggccagcggc actagcaggg cgaatgctct
     3001 gatcagcgca gacacccagc cgaggagaaa cccccttggc aataatagga cttccggaaa
     3061 aactaaagcc accaacaaga gcattgcggc attccggcta gacgtataca tgaagtgtag
     3121 ccccccgccg atcaggagtg aaatcacaat cacaccaagg gccccaccga ttacgtaggc
     3181 ctccgcgata tatgcgcctg aagtggcgaa gccttggtca aacatggacg gattcaggaa
     3241 aactgagaca tcaaagccga actgccgacc acgaaagtaa gtggagacat cactcgcgac
     3301 aaactcgatt tccagctcgt gtagcaggta agaaaccaca taaggtttga acaggtttcc
     3361 atgtactact gcgacttccg tgacggccag tgaagcaccc tgcgcggcgg taaaccccac
     3421 cgggtcgatc gcggaacggc ctttgacatc ttctccaaaa cgcgcaaggc ctatcagatt
     3481 agccaccacg accagcgcag caaccagaac caccatgcgc cagatacgag tatcttttgt
     3541 ggacttgatt ctcgccacgt accagatggt caggatgagg ctgaagggcc ccatccgagt
     3601 acccgtgagc aacagcacga cgctgctgca gaagtacagg gccaccaccg tgtaaagacg
     3661 tttctttctt ttttcgaaga cgacaatcgc caccagaaca ggaagggtca acaacgcaac
     3721 ggctcgaacg accaaaggaa cgctagcggc gaggtgaccg tagtcactga agaaaactat
     3781 gtatccgccg tgctcctgga tgtaacgaaa gtacaggtag ttcttgtaga gaaggaaggg
     3841 caggcttgaa aaaaagacca ggtacaggta aggcaaatcc tggcggacgt taacatctgt
     3901 tggcggtggg attcggcggt aattccatcg gcacggacca tagatgcaga tcgcggataa
     3961 ggcaatgcac aacatgacca cgccagcctc gatccttgat attccgaatg gttctgcgac
     4021 cattagttcc accatgaacg ggtctgaaat gctgcccagg caatacccga ccagcctccc
     4081 tccctgaaac aacatcagcg tgcacagaaa caggaagcaa gggtggcgtc cctggccgag
     4141 attgacccac gacatcacga tgagcgaagt gagcagaacg acggtgagga cgaccgcgcc
     4201 attcaccggc agccatccgg cgagaaagca caacgtggcg atgtcgacta ccgccaggta
     4261 cagcagtatc tcgaaggctg cggctggaat gcgcaacgcg ccgtaggatg gaagcgggta
     4321 tgcgggcgcc gccgaactca tagacctccc ggagtatcga gcagtccttt cagatactgg
     4381 ccatagccgt tgttggacat ggtagcggcc agccgttcaa tctgcgcggc atcgatgtat
     4441 ccggcgcgat aggcgatctc ttccggacag gcgatcttga gtccctgacg tttctcgatg
     4501 gtctgaatga aggtcgccgc ctccagcagc gagtcgtggg ttccggtatc gagccaggcc
     4561 atgccgcgac tcatgatctc ggcatgcagc tcgccgcgat ccagatagag ccggttgagg
     4621 tcggtgatct ccagttcgtt gcgggccgag ggcttcaggc tacgcgtcag ttctacgacc
     4681 tgacggtcgt aaaaatacag gccggtcacg gcataacgcg actttggctg ctttggcttt
     4741 tcttccaagc tgatcgcgcg gccattggcg tccatctcga ccacgccgta gcgctcggga
     4801 tcggtcaccg cgtaggcgaa cacagtggca ccctgctcgc ggctggccgc aacgcgcaac
     4861 gtgcctgata ggttgtgccc atagaaaatg ttgtcgccca gcaccagggc gcagcgctgc
     4921 gaggcgatga agtcggctcc gagaagaaat gcctgtgcga tgccctcggg ggctgcctgc
     4981 acgacataac tgaagttcac gccccaacgc gaaccatcgc ccagcaactg ctcgaagcgc
     5041 gacgtgtcga gcggagtgga gatgacgagg atctcgcgaa tgcccgccag catcagcacg
     5101 ctgagcgggt agtagatcat gggcttgtcg tacaccggca acagttgctt ggagacgcac
     5161 tgggtcgcgg gatacaggcg cgtgcccgag cctccggcaa gaatgatgcc ctttaacggc
     5221 gcgttgtggt tcatcgtttc acctcgctcg cgccagccgc cgaccgttgt gcgtattgcg
     5281 tgtcgatcca atcgcgatag cttccgctgg tgacgtcctg cacccagggc tggttgttca
     5341 gataccacgc gacggtcttg cggatgccgg actcaaatgt ctcctgcggt tgccattgca
     5401 gctcgtgctc gatctttcgc ggatcgatcg catagcggcg gtcatgaccg gggcggtcgg
     5461 tcacgaaacg gatgaggttc tcgtgcggct tgaactgcga gtcagggcac agctcgtcga
     5521 ggatggcgca gatggtgcgc accacatcga tattgcgttg ctcgctgcgt ccgccaatgt
     5581 tgtacgtctc gccgggtcgc ccatcatgca acacccggag tatggcggcg cagtggtcgc
     5641 caacgaacag ccagtcgcgc acgtttttgc catcaccgta gatcggtaga gtctcaccct
     5701 tgagcgcgtt aaggatgacc agcgggatta gcttctcggg aaactggaac ggcccatagt
     5761 tgttggagca gttggtggtc agcacgggca gtccgtaagt gtggaaatag gcccgcacca
     5821 ggtggtcgga agccgccttc gaagccgcgt acggactgtt cggcgcgtac cctgtcgtct
     5881 cgcaaaacgg cgcgtcggtt tcgctcagcg atccgtacac ctcgtcggtg gacacatgaa
     5941 ggaagcggaa gcgtttctta tcttccgcgc caaggttccc ccaatactgt ttcgcttcgg
     6001 tgaggagcgc gaacgttccg ttgatgttgg tgcggataaa ctcatccggc cccaggatgg
     6061 aacggtccac gtggctctcg gcggcaaagt ggacgatggc ggagggatga tgatcgcgaa
     6121 agactcgact cacgagagcg cgatcgttga tgtcgccatg tacaaactga tagcgcggat
     6181 tgcctcccag cgacgcgagg ttggccgcat tgccggcgta agtgaggcag tcgaggttta
     6241 caaccgccgt ggattcctgc gccagccact gcaggatgaa gttggatcca atgaatccgg
     6301 cgccgccggt aacgatgatc gagtctgtca taaaggccct aagtagaatt tcgtaaatag
     6361 ttttgtagtt gcgcgaggcg cgcagcttcg ttggggtcct gctgtttgcg gcggatggcc
     6421 tcccagatca acacacccag gcaggacagt aagaacgtcg cgatcgccgt cacggcgatg
     6481 atcaagagtc gcttgggact ggacttccgt tccgccggca cagccttgtc aacaacctga
     6541 accatgactg cattcttgga ctcgtcgatg cgagcggctt ccaattgctt cccgagaaag
     6601 tcataaaggc tctcgtggta cttcaattcg cgcgccacgc ggatgtattc gaggttggct
     6661 tcgggcaccc gccgcgtcgg aacctgcaga tttccttcgc caagatcggc cccgctacgt
     6721 tccatttgcg acaacttggc ggattgcgcc tgcagttcgg ccaactcccg ctccacccgc
     6781 ttcacgtcgg gattgtcctc cgtcgcgtag gtgcgcagtg actgcaattg cacctgtttg
     6841 gcagcaacct gggcgcggaa atctgctacc gcgccaatga gcgcgcgagc ctgcgcgtcg
     6901 ggctgaatca gtcccgtctt ttcctgcagt accttaagcg ccaactccgc cttcgacagg
     6961 tcctcgcgct ccgcgactag cttttgttca aagaagacgc gtctctgggc gccggaggta
     7021 ccttgtggcc agatcgtcta gtgccaattc aggatccgac gtgaccaggc cttttggcgg
     7081 ccccagaacg actccattag cggctgacct aagttcctgc aagagctcgc caagcggggg
     7141 gaacttacgt tcaatggagg actgctcaag cctggccttg atttccctaa cgatagcgct
     7201 gattacttct agatcgacat cactaacgtc atggttcaga tcagtccagt cctccagaaa
     7261 tcgcacaccg gcgaacggaa gctcacggcc tgatctctca gctgagagag tctgatcttc
     7321 aagcagtatg actccaattt tcacctcgtt cccggacgcc gcgttgggaa cgtactttac
     7381 gatgtaatat tggaattctc gttgggagct catacacgtt gttgcgaacc gacaatgatc
     7441 gcacttcaaa atctacacgg caactcaacg cagtgcaaca cgtccgcagg atgcccggcg
     7501 gcgcgcagag ccaactgtta gagtgttcgg acggttgcca ttatgttgtc aagttccaga
     7561 acaatccgca acatccgagg gtgttggtca atgattggct gggcacacgg ttggctgcgt
     7621 cgatcgggtt accagtgcct acggttgccg ttgtccacgt cgaccagcaa cttatcgaca
     7681 gcagtcctgg tcttattcta gaaacccgag gacggcacag cgtcattgct cctgggctgt
     7741 gcttcggctc tcgttatgtt gtgtccccgc ttgaaggaca ggtatacgac tacttgccgg
     7801 aatcgatgat ggtgcacgtc cgcaacctgt cagactttgc gggtattctg ggtctggata
     7861 agtggacatg caactgcgat ggtcgacaat cggtcttctg gaaacgcaac cgagagcatc
     7921 ggttcactgc cagtttcatc gatcaggggt tctgcttcaa ttccggcgaa tggaatttcc
     7981 ccgacgcacc gctccggggc gtatttgggc ggaatgacgt ataccgttcc gtgacctcat
     8041 gggattgctt cgagccgtgg ctgagtcgag tggagaacct gccggaaagc ttggtgtggt
     8101 ccttggcgca ggagataccg ccggaatggt atggctccgc cactgaagaa ctggagcgcc
     8161 tgctctccca attgctgacg cgaagggcgc gggttcgaca gctaattttg gacttcaaga
     8221 actcgtcacg gaaccccttt ccaaattggc gcgagatcga gaattagctt gctttgattc
     8281 gagccccgcc caaagaccgc gaaagcggac tgcgtcacat cacagccagt tcggacatgt
     8341 tatcgatctc gtagcgcaca ctctctcggt cgcgaacatt cgcgactagt tcggtgtcag
     8401 accagtttgg aaatatatcc ggattggcaa agaggaaccg cgagagcaaa gggcgaacga
     8461 tttcttgtcg tttgcgcaac tccctcaaga acggagacac ccaattacat ccgtcgcacc
     8521 atatcgccgg aatctgtccc gcaatacctt ctaaaatccg gctgttgacc gctcccactc
     8581 tgctcaaaaa aggctcaaag tcgcaccatt cgctgatgtc ctgatacacg gccttgtgac
     8641 gatacattgc cactctcgca gggtcaaatg cgcagccgat cggatcgcat tttcgaggtt
     8701 cccaaccgtg atacaagaac gccgcattat aaagagtgtc atgtgaagac cgacaaaata
     8761 cggtctggcg ggcacctgcc cttttcatcc aaaggtcaaa tgcgagagcg ccggcgaata
     8821 cgttgagatt tcctaccctc gacagggctg tctccgggaa gtagtcgaag agtttgccgt
     8881 cgcgcggctc caccagataa cgagagccac actgcagagc tacttttttt ctagtcgtcc
     8941 catgaaagtc tttcgcatcg ctaaacattt gaagccacgg cgacaccgat atgagtccat
     9001 cggagtggtc gatcaacccc tgctcgaccg caacgacctc gacgtccggc gtcggtaacc
     9061 ctatcgaact agcaagtttg caggtaagaa acttcgtgcc tagggatgcc gaacgatccg
     9121 taatcccttg aaatgtgacg acgtaatagt gcccatcaga gcacctgaaa actgccgagt
     9181 tggaactctc gttaatcgta cagacgaatt ctgtggcgca aatcatatca atggactcca
     9241 gtgcatcgtg ttttcagaac atagccacac gacccgagtc gggttatgtg tgtactccaa
     9301 tgttatgagt cgacgaagct agacgcattc aaaaaatgac atgcattgtt acgaacacgt
     9361 tacgaaggta acacccggga agcgaaggac attaggcgat gcgggaagtt gaaaaagatg
     9421 gaggacccag ggtttctggc aactcggcta tccgtacacc gtgactgtca acggtaacct
     9481 gactaccgta atgtgcgacg actgggccca cggtggcgca cctggcgata cctggcaggc
     9541 gaacttcacc aacttgggca ccagtaacct gagcctggtg cgctttaacc agttgccttc
     9601 ggcactgact ctctacgacg aggccggctg gcttctgttg caaaccaggg tcactcctca
     9661 agctcagtgg aaggacatta attacgctgt gtggcacatt tttgacccga atgctccgct
     9721 gcctggaacc gccccgctct actgggtgaa cttggcgcag caggaagcta cgctcggctt
     9781 cccgggtgtc gacttctacc aggtcggtat cttcacgcca ctcaaccagc acgataccaa
     9841 tctgaatggg ccccaggagt ttttgaatat agtcccggaa ccgagcacgt tgcttctctt
     9901 gggaacgggc ctcgccggtt tgcttggacg caagtggctg acctagctat cctggttccg
     9961 cctagcgaat cgagttgtta acgaaactgc tgacttcggt cggcagttgt tgttgatccg
    10021 ctgcttcgtc taacctgcga gctcctcgcg tgtaattagt gcggggatcg tggcttgagg
    10081 tctggccaag cggtcatcgg cggtctatgc tggccgatct ctacagggga ttcggaatgt
    10141 cttggcatct tgcagcagcg ggaagacgca tagctgtggc cctaattgca atgagttgcg
    10201 ggctgggttg tctgggtacg accgcgttcg cgcaaaacgc ttgtcggccg gaagacgaaa
    10261 tctttggcgg atacgccttt ctcttcccca acggatgggg cgacctcgac tacaaaatca
    10321 acaatatccc aaacgccttt gacgcttcca atgcttacta ccttcggaac cccccaattt
    10381 cggactcctc ctggatggca gcggccactt tttcggtggc actacgcccc ctaatcttgt
    10441 caatggatcg gatgactcca ccggagtgtc ctatgcactg ggaggggtac agtacaaata
    10501 ccacaccgat aaattgtcgc ccttcctgcg caccttcgtc ggtgcggcca gcatctcccc
    10561 agactgttgc ggtggaaacc agtggaatct cgcggctggc ggaggcggag gcttagacct
    10621 cgcggtcagt ccccgattct ccatccgact ggcgcaggtg gactatatct actccaacta
    10681 ccgtcaccga ttcgtcaccg accatgcgac gcaatggaat agcgttcgcc tggcggcggg
    10741 tgtggtgttt aatgtgggca gttactgcaa catcatgcct ttgtcctgct cggttgctgc
    10801 ctcgccaacg ccggctgaag tattcgcggg cgagccgatc aggctcaata ccgccggtac
    10861 taacttcaac tcgaaacatg cagtgaatta tagttgggcc gcaaccgggg gtaggttgtc
    10921 gagcccaacc actcaagcga ccgagattga caccacgggc ttggcgccgg gaagctattc
    10981 cgtcaacgcc accatgaccg atccgacaat taagaaggtg aactcggcca cctgcgcgac
    11041 aagtttcatc gtcaagcagc cgcctgcctc ggttccaccg ctcgtaagct gctcggtcga
    11101 tcccgccagt atcgctgtcg gagggtcggc aactgtgacg ttggcagcca gtagccccga
    11161 tcgccgtccc ctgacttaca gttggtcttc tacgggcggc cagttgagcg agcaaggcgc
    11221 gtccgccatc ctcaccgtgt acgcagcgga tgcgggcaaa accatcgcgg tcaccggaac
    11281 tgccacagac gaccgcgcgc tcagcatgag cagcacttgc aatgtcgacg tcaaggcgcc
    11341 tttgaccccg ccttgcgtca aaattgagga ttggggcgaa tgtaccttcg aaaaggaccc
    11401 caagcgaccg tggcgtgtcg acaacgattg caaggatgtc ctcgacaaac ttgcccttcg
    11461 cctgcgggag atgccaaccg gcaatctggc gatagttggt tacgccgagg agaaggaagt
    11521 cgtcagcaac cagaccctgg gagtccagcg ttcggtgaac gtcaagcgtt acctgactac
    11581 ggacggaccg accaagagcg acgctgcacg catccagcct cgacagggcg ggaccaagga
    11641 tgcggtcact cacttctact tcatacccga aggcaaactg tgtgctggtg agctggaaag
    11701 ttttggtagc ttggtcgacg aaaccaaggt cacgggaagt tctcgcgacg tgcctccccg
    11761 aggcaataaa gccgcgacac ccaagccacc ggtttcccca tcccattagc tgggcgtcta
    11821 acaacacgac gaggcgggta agagcggtca gactacctgc ctcgtcgaca accttcagcc
    11881 gacgccggca cattctcgac gcgccgccgc tcttattctg ggctgtgacg caaatactaa
    11941 atcaactaat ggtgcgaatt aatgcgggaa gttgaaaaga tggtggacct gagcgggatc
    12001 gaaccgctgg cctcctcgtt gcgaacgagg cgctctccca gctgagctac aggcccctct
    12061 ttggctggat acaaggtctc cgccagattg cacaaggcga accaacctca cgtcactcat
    12121 tatacaaaca atcatcaccg catgaaaatg cggcggtcac gccttgcggc cgtactcatc
    12181 caacgcctgg ccgagtcgct ccaggcccat tcgcgtcatc tccggatcgc cgcctatgcc
    12241 aatgcgaaag tgttgcggca tgtcaaagta gatgcccgga accacgctgg tctcgtattt
    12301 ctctcgcagc agaaccacaa acccatcaac atcgcctgag cgtagtttcg gaaacacaat
    12361 ggttccctcc cccggacgat agaaatccaa gtccgtccgc gagtccagga aggcgttcaa
    12421 cgatctgcgg ttttccgcca gcagattttt cgcgcgcgtc gcgactttgt ccagatgatc
    12481 aagtgcaatc acgctcagca actccgcggg atgcgccgga gttgctgcat agagatcgtt
    12541 aatgtgccac atcctggcgg taatttcctg actggcgaga acccatccgc accgcaggcc
    12601 gctcaagccg aacgctttgg tcaggctgct cgtcaccaca aactgatcgc ccagtagaaa
    12661 tgccggtcgc ggacgctctt cgtaaaattc ctccaagtag acttcatcca ccagcactcg
    12721 tgcgccgtga gacttcgcga tttctcccac ggcacgcacc gtggcttctt cggcataagc
    12781 gccagttgga ttgtgtaggt ttgtcagaat aatcaacttg gtggcaggtg taatctggcg
    12841 ctcgacttcg gacggatcga tgcgaaagcc gtcttccaat cgtcgctcga atcgtcggat
    12901 ctgcgcaccc aggtaaaccg ccgtagactc cagcaattcg tacgtcggat gctcgatcaa
    12961 gacctcatct cccgggttaa aggttgcggc catcgccatg tggttcgcca tcgatgtccc
    13021 tgccgccgca aacacgcact ccggcgggac cccgttgtag cgcgcgaggc gctcctgcag
    13081 cggagcatag ccatagactc ccgggccatt gatctcgagg tcggtgaggc gcaccggcaa
    13141 ctcagacagc ggataactca tgattccgct ggtggccagg ttgtatttcg cgccggaacg
    13201 cagtttggca aactccatgt agggtgaacg catgtgcgta cgtggatgca tttgaggtct
    13261 ttactccaat cccttctttc gccgcgccca gaatgcatac acgggcagtc cgagcgcaag
    13321 aattcccatt cccaacagag tgttcgccgg gtatttgtaa attgtattgg ccaccaccag
    13381 ccagcaaatg gcgacaaaga gtccagtggt gtacgggtgt ccgggaactc gatagctgcc
    13441 catcgctgtg tccggcaaag atgttagctt cgcttctcta aggcgaaaaa cgaacagagc
    13501 tgcgccggtc agtccaaaga aaatgaaatc catggacacg acgtaattga gaattttttc
    13561 gtagctgccc gagagcgcga tcaccagcgt caacacactc tgcagcacga tggccagcac
    13621 cggcacatgg gacttcttgt tgactcgtgc aaccgccttg aagaaaagtc cgtcgctcgc
    13681 catggcaaag tacacgcgtg gtgccgtcag gatcgactgg ctgagaaagc cgagggttga
    13741 aattgcgatt cccagggcaa tcaggcgcgc gccggtattt cccatggcca agtgcatgac
    13801 cgcggatgcc ggaaccgtgc ttgccgcaag tccctccgct cctagcgccc gtacgcaaac
    13861 gaaattcacc ccgaggtaca gcacaatcac tccggtcacg cccatcacta acgcacgcga
    13921 caaattgcgt tgcgggtgct tgatttcggt cgcgatgaag ttcgcggtct gccagcctcc
    13981 gaaagcaaat agtaccggaa ccatggccgc gccgatcgcg gtggcaagat cgatggaagg
    14041 cttgcggtcc agcaccggat gcagcattgg gtaaggtgcg cgaatcagga acagtcccgc
    14101 cagcaccagg gttgcaatcg ccccaatctt cagcagcatc aggacgcttt gcactcggct
    14161 accagccttt actccgagac agttgatggt ggtcagcagc aagattgtcg tcaccgcgac
    14221 catgcgctcc gatacgggcc agttcgtcag ctccagcagg tatcttgcaa aggtgacggt
    14281 gaccgccgcc attccccccg tttggatgac cagcagcagc acccatccat aaagaaaccc
    14341 gacgatagga tgaaatgcct ctcgcaagta ggcgtactgg ccgccgacgt cgggcatgcg
    14401 cgccgccaac tcggcgtaga tgaacgctcc cagcatggcg acgaccccgc ccgcgaccca
    14461 aacaccgaga atcaacatgg gcgtgtgtac ctggcgcgcg acgacgtaag ggttaatgaa
    14521 aatacccgcg ccaacgatcc cgcccattac gatcatggtg gcgtcgaaca agccaatctg
    14581 ccgcagcagt tggactggcg ctacgctttg cgatggtgta gcttcggtgg gttcggacgt
    14641 catcattttc gccggaatgt gcgaatgtaa cacgcgtgcg gtgcggggcc aatcgttcga
    14701 caggattacg ctccggaagt cgctgcacgc cgcaatcgat cgagcacacc ctcccactcc
    14761 acgccgggcc cggtaatttc gaccgcaatc cagtcgtagc cgtgcgcgtc gagatcgtgc
    14821 agcacagtgt agagtcgcgc agcatagtcg gcggcattgt ttggcatggc tactacttcc
    14881 cgcaccgacg ctacgggcgg atattgaatc tgcagataag ctccggcccc ttgcagcggc
    14941 acctgaccac cgtgcacgag atatagcgga gtttgtgggc tgtagtgacg cggatgcatc
    15001 cccggtgacg gatgcgctcc cgtcggttca tgctcggatt gggcgattgc cccgatcaac
    15061 tcttcaatct gccgtcgcga aatacctccc ggccgaagta atactggatc ctcgtcaacc
    15121 agcgatagca cggttgactc gatacccacc tcgcacggtc cgccatcgag aatgtaagcc
    15181 acaagttcgc cgaggcccaa ccgcacatgc tcggccgtgg tcggtgagat ctgcgtaaat
    15241 cggtttgcac tgggcgccgc aacgggaacc tgcgctgcct cgattagcgc caacgctacc
    15301 ggatgcgccg gcatgcgcac acccaccgtc ggcaaaccag aggtcactct gtctggaact
    15361 tcggttgttt tgggaacaac cagcgtcagc ggtcccggcc agaatcgctc ggctaggatg
    15421 cgcgccttct cggaccatga caacgcgacc cggccgatca tctcaaaact gctgacatgc
    15481 acaatgacag ggctggtgtt cggccttccc ttgacttcaa atatcctcgc caccgctatt
    15541 gcatcaagcg cattcgcacc gagcccgtaa accgtctcag tgggaaaagc gaccagcttt
    15601 ccgcctcgaa gtaattcggc accgtgcttg atctcgtttt cggatacacc gaattgttca
    15661 atggatgtca ctagacgcca caatcgttca atgtttactg gctacgtcca tgcgaagggt
    15721 ccgcagagtc cgccgaatcg atatctatgt gcagtccagc atttggtgca atgcccggcg
    15781 gataacaatt gttgtctggc gcatacgtct gccggcaagc cgcggtcatc tcggggcgcc
    15841 gcggcgattg ggcatactgc caaattttgg cgaacgtaac tccgctcttg gcgggagcgg
    15901 gtggcgcatc aggaaataca caaccgggcg ccgggccgca ttggtcattg ctgatgaaga
    15961 aatggatgtt gcgccccgcg gcgttgtcgt ggatatcgtc ggcggtcacc accgatactt
    16021 tgctcgattc gcgaaaggca atccctgagc aatacacgcc cgcgccatag ccgcccttca
    16081 ccacaccgtc gatccatgcg tgaatgtagg cgcgctgctc ggggagcatc cgcccgccct
    16141 gctcctggtc gagaaaaatg attgctccct tcggaaaacc ttctgaagtt gctgccttca
    16201 ccgccgctgc cgcatcgcgg aaaccgacac cgggtgcatc ccccgaggct ttcagctctt
    16261 gatattcacg tccgttgaag agcaacagga atccgtagcc gagtgcatgt aagctggttc
    16321 gcttccctac ccacgtattg cgcgcagctc cgggaggac
//
LOCUS       DASRKK010000100         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_885784, whole genome shotgun sequence.
ACCESSION   DASRKK010000100 DASRKK010000000
VERSION     DASRKK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885784"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFC15_02185"
     CDS             <1..259
                     /locus_tag="VFC15_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM08999.1"
                     /translation="STHRRESTVSGSTNMATVPTEKHPYTQVPDGKTLISFEEAMNVL
                     SRDERFKATVYAMNTLLVQKGIYSPDEFEFHFRQWAQKVKK"
     gene            593..1330
                     /locus_tag="VFC15_02190"
     CDS             593..1330
                     /locus_tag="VFC15_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09000.1"
                     /translation="MSSTSQAVTRTPAEVVRMAPVSQAANGICYSVMGEITTAVADLE
                     RMVNAVPRTIAAALNRKAYYFVPLTLNMGDDTMIADRYDVQLSDSAVCHRNQTLGDSQ
                     CVFISTRLMEDSFAIAFEFFINVGHNFVERVGVSQEFADLVWKQVDEKARGETSLDAY
                     ELRKVATTEGPEAEKAKHEYLLAAFADAIAVYTLSLYLDVDYQDLRERDYPLLAPNAL
                     AERLRKINELFPANPGFEFAVYHRRRQ"
     gene            1334..1798
                     /locus_tag="VFC15_02195"
     CDS             1334..1798
                     /locus_tag="VFC15_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09001.1"
                     /translation="MLNAWAAAKQWLLFSQSNTDPKRTPQFFQFVSTWIGFNALYSTD
                     RKRGNPEPIVVSQFAFEQLERHHLHLLESDQEYIQALEYLQIRPVKNMRTGKPVEITD
                     RKDIRQVADCLYAVRCNLFHGDKDPGELRDQTVVDRASVVLSHLLTAYLGSR"
     gene            1839..>2119
                     /locus_tag="VFC15_02200"
     CDS             1839..>2119
                     /locus_tag="VFC15_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09002.1"
                     /translation="MNRMILANLVHRPFRTIISVVAVAVEVTLILLIVGIMLGMLNDS
                     KTRQAGIGADIMVQPPGSSLLMGVSGAPVSVKVADKLRKLPHVVAVAPVV"
BASE COUNT          464 a          633 c          568 g          454 t
ORIGIN      
        1 ttccacgcat cggagagaat caacggtatc ggggagcaca aacatggcga cggttccgac
       61 tgaaaaacac ccttacacgc aagttccgga tggcaaaacc ttgatcagct tcgaggaagc
      121 gatgaacgtg ctgtcccgcg atgaacgatt caaggcgacg gtttacgcga tgaacacgct
      181 gctggtgcag aagggcatct actcgcccga cgaattcgaa ttccatttcc gtcagtgggc
      241 ccaaaaggtc aagaagtaga ctttttcttc tgtcggactt cttctccaaa tcatcccact
      301 tcggcatgtg gcattctttg tgcgagcacc gttcgtgcgc gccgatctca cattgcgaac
      361 actcgcattc gcaccaatgc tcgtactggt cgcgttctcc tttcctttct ggcatcgaat
      421 ctccttcaga ttcccattcg aaacttgtaa tctacactac ctacgatgct atacagacct
      481 ttgaacttga caatgactta tgaggttcta aacggagatt cttaggtgga acggcacggc
      541 attggcctat gcggaccgcc gtcccacccc attgcgttac aatcaagcct ccatgtcttc
      601 cacctcccaa gccgtcaccc gaactcccgc tgaagtagtc cgcatggcgc cggtgtcgca
      661 ggccgccaac ggcatctgct actcggtcat gggagagatc acgacggcgg tcgcggacct
      721 ggagcgcatg gtcaacgccg tcccgcgcac cattgccgcg gcgctcaacc gcaaggccta
      781 ctactttgtc ccgctcacgc tgaacatggg cgacgacacc atgatcgccg accgctatga
      841 tgtgcagctc tccgacagcg ccgtctgcca tcgcaaccag accctgggcg attcgcagtg
      901 cgtcttcatt tcgacccgcc taatggaaga cagcttcgcc attgcgttcg agttcttcat
      961 caacgtggga cacaacttcg tggagcgggt tggcgtttcg caggagttcg ccgacctggt
     1021 gtggaagcag gtggacgaga aagctcgcgg cgagacctcg cttgacgcct acgagttgcg
     1081 caaagtcgca accactgagg gacctgaggc tgagaaagcc aagcacgagt acctgctggc
     1141 cgcctttgcg gatgccatcg ccgtgtacac gctgtcgctc tatctcgatg tggactacca
     1201 ggacctgcgc gaacgcgatt atcctctatt ggctcccaac gcgctggccg agcgcctgcg
     1261 caagatcaac gaactcttcc ccgcgaatcc cggattcgag tttgccgtgt atcaccggcg
     1321 gagacagtaa gctatgctga acgcttgggc tgcagctaag caatggctgt tgttttcgca
     1381 gtcaaatacc gatcccaaga gaacaccgca gtttttccaa tttgtctcta cctggatcgg
     1441 gttcaatgct ctttactcga cagatcgaaa gcgcggtaac ccggaaccga tcgtagtcag
     1501 ccaattcgcg ttcgagcagc tggaaaggca tcatcttcac ctgctcgaaa gcgaccaaga
     1561 gtacattcag gcgctcgagt atctgcagat ccggccggtg aagaatatga ggacgggaaa
     1621 accggtcgag ataacggacc gaaaggacat ccgtcaggtt gcagattgcc tttacgcggt
     1681 gcgctgcaat ctattccatg gagacaaaga tcccggcgaa cttcgggacc aaaccgtcgt
     1741 ggaccgtgca tcggtggttc tgtctcattt attgactgca tacctaggca gcaggtaaag
     1801 cggcacgcgc atcaagcagt gaaatcgagc ttccaaccat gaaccggatg atccttgcca
     1861 acctggtaca ccgcccgttt cgcaccatca tcagcgtggt cgcggtggcg gtcgaggtca
     1921 cactcatact tctcatcgtt ggcatcatgc tcggcatgct gaacgacagc aagacccggc
     1981 aggccggcat cggcgccgac atcatggtgc agccgccggg ttcgtcgctg ctgatgggcg
     2041 ttagcggcgc gccggtctcg gtgaaagttg ccgacaaact gcgcaagctg ccgcacgtgg
     2101 ttgccgtggc tcccgtcgt
//
LOCUS       DASRKK010000101        10007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_397214, whole genome shotgun sequence.
ACCESSION   DASRKK010000101 DASRKK010000000
VERSION     DASRKK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397214"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFC15_02205"
     CDS             <1..626
                     /locus_tag="VFC15_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM09003.1"
                     /translation="SETTISRWMKRAPKDPQPAKRWLAFLRNHREAIAAMDFFTVPTL
                     TFGVLYCFFIIGHDRRRILHFNVTRHPNSIWIVQQLREAFPYDSAPRFLLFDHDTKYG
                     LEVPASIRSLKMTPVRTSIRSPWQNGVAERWVGSCRRDLLDHVIALDERHLKRLLSQY
                     VSYYHDDRTHLGLEKQTPAARARSRENGTVICQQRVGGLHHRYDWAA"
     gene            1414..2502
                     /locus_tag="VFC15_02210"
     CDS             1414..2502
                     /locus_tag="VFC15_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZM09004.1"
                     /translation="MNRREFVRNTSFAFAGFTLSKAEPSLAESPPPDGWRTFEVTTRT
                     EVLKPAGETKVWLPAALIQDTSFQRTTSNRFNADGGTARIVESSPNALGIVVAIFPSE
                     AKPVLTLTSQVALKNYAVDLSKPGNVRAAPKAELQFYLQPTQHMPTDGIVKAKAIEIT
                     KGANTDVEKARAIYDWIVDNTFRDPKVRGCGRGDIRFMLESGDLGGKCADLNALYVGL
                     ARASGLPARDVYGIRVAKSELGYKSLGASSEKVTKSQHCRAEVYLAGFGWVPVDPADV
                     RKVVLEEPPGNRPLSDEMVISARKRLFGSWEMNWMAYNFAQDVELPGSSGTTLPFLMY
                     PQAQTSEGKLDSLDPENFKYEITARELS"
     gene            2517..3011
                     /locus_tag="VFC15_02215"
     CDS             2517..3011
                     /locus_tag="VFC15_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZM09005.1"
                     /translation="MNVSTTRDSVRHMPAANGLAVIRVTIGAMFIWVFFENLGKGLYT
                     AAGYAGLIQYYVQHSHSPAAWKAIMGLAASHAAMAAPLQGLTEISLGVLLLIGLFTRP
                     AAFVAFLFLSSLWLSEFGTSWIWELLVPVMASLGLAIGRAGRAWGIDGWLERRWPNAP
                     WWLL"
     gene            3022..3711
                     /locus_tag="VFC15_02220"
     CDS             3022..3711
                     /locus_tag="VFC15_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM09006.1"
                     /translation="MRVSVIIPTHNEAQAIGRVLADLPASMVTEVLVVDSNSTDGTAE
                     IAAAMGARVLREPRRGYGRACLTGLANTDSPDVVVFLDGDYSDRPDELSLLLAPIAEG
                     KADITLGSRLAKKNVRGALPWHSAFGNRLAAGMIRVLYGQNISDLGPFRAARADVLRQ
                     LALEEETYGWAVEMILKGSLAGFRIVEVPVSYYPRIGKSKISGTLRGTAGAAWFILSR
                     IVSYYFRRRKA"
     gene            3735..4460
                     /locus_tag="VFC15_02225"
     CDS             3735..4460
                     /locus_tag="VFC15_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZM09007.1"
                     /translation="MVSSDTLPHSRSNDRTLVIMAKAPRPGMVKTRLARSLPVEAVTD
                     LYRCLLGDTMALAHSLSSGKVAIMCPASDVEELTQFAGDTTGVVAQQGEGLAAGLTSV
                     FAQFALGGHCVIAFNSDSPHLPVSVLERAFEMLTVHDVVVGPTHDGGYYLVGAKAPHA
                     TLFDGDGMGTNSALDGLLARARRLQLSVGFAEPFYDIDVEGDLTQLAEDLRLAPARAP
                     RTAAWLKEWQQRAPQLRNAAGGL"
     gene            4457..5746
                     /locus_tag="VFC15_02230"
     CDS             4457..5746
                     /locus_tag="VFC15_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZM09008.1"
                     /translation="MKLNPSHRLYGLGAVLLVTLTICSRKFGGEGEAFPIIPLLVAGV
                     AYLLAVREFFRIPNFPKRVVVIGLVLASVWHIQFLRIPTGSDDDVRRYVWDGRLQRLG
                     YNPYLVVPDDPAFAALHTAETQTLNNPDLPSIYPPGAQLFFRAVTAIHESAFALRLAF
                     AVCELAMVLVLLDILRGTGQAAHWILALAWNPLLATEAVGSGHIDILGALLLVVSFAA
                     LIRRWRAVAAVTFALAVAVKFLPVVLLPLYWRRVRIRDGLLAVIVFVLLYLPFLNHGR
                     IPIGSLGTYIHGFGFNDPVFAVLRRMAAPQILVGLAVFVGLLIATWYRIRSRALSGDA
                     FAWPMAASLLCAPAVFPWYLLWLLPFARSASTLPIIIWTVSIIPTYIVWHLRSLGHPF
                     VLPGWVMLLEYGAVGIAAGAVLIRRIIRPAVPKCPAD"
     gene            5857..6594
                     /locus_tag="VFC15_02235"
     CDS             5857..6594
                     /locus_tag="VFC15_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09009.1"
                     /translation="MTDSLCNTAVRLFLVFGLLVFAATSQAQQHNPILEKLAKTYGID
                     SWDKIEAIRYTWNGEITGLFKLSRTWTWEPKTNKVSYQGKDQDGKPVDVTYLRSEVSS
                     QPDAVKKVDSGFINDNYWLLFPFHAYWDTSATVTNQPMQKLPIGTGSAELVSVKYPSE
                     AGGYTPGDTWNLYVGKDSRVEVLEFHHGGNAKPSLVIATWTGYKKAGPLLFSTDHRGT
                     ADGKPLHIFMTNVAVKLVGSDKWMPAQ"
     gene            complement(6606..7661)
                     /locus_tag="VFC15_02240"
     CDS             complement(6606..7661)
                     /locus_tag="VFC15_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM09010.1"
                     /translation="MRRLIAQSSSSGPRMTPGAGIALGLGLTNECNLACAFCYRDPTR
                     TDRLSLDQVKEVMERVAVRSVNLGTGENGMHPDFKAILAYLRTQPVKLTITSNGHSIA
                     VLEDSELRAFHDIEFSLDYPTESEQDAQRGRGNWALIHQQAERCLRLRIPVTIIAVMM
                     KANYLRLVDVAQVAKQFDAPLRVNVYQAVRSDVYALSYEEYWEGFRRLFEKTDVIAIG
                     EPLVRAMAGLPPLRGGCGVSTIRVTPRGTTQPCVYWPGSGGPLSELISMGPDVLDSAP
                     FEQARKLPDACRPCEFRESCHGGCAGRRRLQGALAQPDFYCPIIRGERPRLEIRMAAK
                     RDLPKLKSSCTTVVIAR"
     gene            complement(7658..8473)
                     /locus_tag="VFC15_02245"
     CDS             complement(7658..8473)
                     /locus_tag="VFC15_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM09011.1"
                     /translation="MTTKDDFERFRTGTAKYAAYLATPEGRLRLDLTFANLQDFLPRA
                     TQSLLALDIGCGTGAIALRLAKLGLHVTLLDASVPMLDFAKRAAEEAGITKSIALMHG
                     DASQLANLFDPESFDVILCHNILEYVHDPCAVLRSAARVLRDPSSVISLLVRNQAGEV
                     LKAAMQDGDLDATEHNLTATWGHESLYGGPVRLFTPQGLQAMLLESALTVIAERGVRV
                     ISDYLPPRVSRNDEYEQILGLERKLATRPEFAAVARYTHCLAHRAGPVRKKDA"
     gene            8831..9793
                     /locus_tag="VFC15_02250"
     CDS             8831..9793
                     /locus_tag="VFC15_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZM09012.1"
                     /translation="MRFLVLFLLSITSGLTFAQDYPTKPVRIIEPFGAGGGPDLVARA
                     ISPKLSELWGQPVTVENHPGKGSTEAPALVAKSPPDGYTLLVNTSAQAYSAALVKTLP
                     YDPLKDFIPVAPLTSQPYVLVAGKSSGITSVGELIAAARAKPGELKFGSTGVGTGTHL
                     GIEKFNLEAGIRAAHVPAQPGEAIADVIANAVAGRTTYIMAPIQLALVDIRAGNLRAL
                     GVTTKKRSSLLPEVPTISEAGVPGFNHPIWYGVWVPAGTPAEVVDKLAKDIARALEAP
                     DVRDRLAKHGADPMSMTQPEFARFVVSESENAARIAKAVGIKPQ"
BASE COUNT         1988 a         2885 c         2944 g         2190 t
ORIGIN      
        1 tgtcggaaac caccatttcc cgctggatga aacgagcgcc caaggaccct caacccgcca
       61 agcgctggct ggcctttctg cgcaatcatc gggaagctat cgctgccatg gatttcttca
      121 ccgtccccac tctcaccttt ggggttctct actgcttctt catcatcggc catgatcgca
      181 ggcgcattct gcacttcaac gtcactcgcc atcccaacag catctggatc gtgcagcagt
      241 tgcgagaagc ttttccctac gattccgcgc ccaggtttct cttattcgac cacgatacga
      301 agtacggctt ggaagtgcca gcgtcgatcc gttcgctgaa gatgacaccg gtccgtacct
      361 cgattcggag tccctggcaa aatggcgtcg ccgagcgttg ggtcggaagc tgccgccgcg
      421 acttgctcga ccacgtaatc gccttggatg agcgccatct gaagcgactc ctctcccagt
      481 acgtttccta ttatcacgac gaccgaactc acctcgggtt ggagaagcaa acgccggctg
      541 ccagggcacg ctctcgggaa aacggtaccg tgatctgcca gcagcgcgtg ggcgggttgc
      601 atcaccgcta tgactgggct gcttgaatcg gttgtacgca agctttcatt tgtctgggcc
      661 catgacagaa gtgtccgctc attcagccgg ttacgaagga gtcccgcgcg tctggccatt
      721 caacgccaca gaaaagttag gtcctgatgt cagggaactc ttgagtatgc actgctcctg
      781 gtgcgtttta tattgtggcg aggcacagtc tgccgtagca ccaagcggcg cgccgcaatc
      841 gtcggtgact tgatcttagg ccgttcgtca tagcggtttt ggcaagtctg ccaaagaaag
      901 ggttaaaccg ccctgagaac tcagcggtct tctccattgc ctccgccggc tgtggctttg
      961 cttcgaacat tcatcagttg atcatcggca gaagcattca aggtgtcgga gctgcactag
     1021 tggtgcccgg aagtctcgtg attggcagtt cctgtttcga tgaaaagagt cgtggccgag
     1081 cgattggttg agcatgcttc ctgacgatgg gcattcttca tcaatctccc gaatcggaca
     1141 acaagagtag cgctccagtt cgtcaaaggg ctctgcaaaa gagtggtaca atttcgcaat
     1201 tcggggcgtt cgcaatacgg gcattcccgt cctaattcta gttgatggct gcgcatcgtt
     1261 gtgttttgct gcgccgttgg gcagcgaaga gcgtttgcgc gcgtcttgtt tgtttttata
     1321 taggctcacc gttctgcagc cagttgagcg ttgcatcgcg tggtgcaacg acggcggtaa
     1381 ccatcttgca tctcttcgag tgggagttag ttcatgaatc ggcgcgaatt cgtacgcaac
     1441 actagctttg cattcgctgg cttcactctc tcgaaggcgg agccgtcatt ggcggaaagt
     1501 ccgccacccg acggttggcg gactttcgaa gtgaccactc gcaccgaagt gcttaagcct
     1561 gctggagaaa ccaaagtctg gctgccggcg gcgcttatcc aagacacgtc gttccagagg
     1621 acgacatcca accggtttaa tgcggacggc ggcaccgcca ggatagtcga aagcagtccc
     1681 aacgccttgg gtatcgtggt cgcgatattc ccatcggaag cgaagccagt tctgaccctt
     1741 accagccagg tggcgttaaa gaactatgca gtagacctat cgaaacccgg taacgtccga
     1801 gcggcgccga aggccgagct gcagttttac ttgcagccga ctcaacacat gccgaccgac
     1861 ggaatcgtaa aggccaaggc gatcgaaatc acaaaagggg ctaacaccga cgtcgagaaa
     1921 gcccgtgcga tctacgattg gatcgtcgat aacacgtttc gcgatcccaa ggtgcgcggc
     1981 tgcggtcgtg gcgacatccg cttcatgctt gaaagtgggg acttgggagg caaatgtgcc
     2041 gacctgaacg ctctttatgt cggactcgcc cgtgcatcag gccttccggc gcgggacgtg
     2101 tatggaattc gcgtggctaa atccgagcta ggctacaaga gcctcggtgc ttcgtcggag
     2161 aaagtcacga agtcacagca ctgccgggcg gaagtgtacc ttgcagggtt cggatgggta
     2221 cccgtagatc cggccgacgt gcggaaggta gtcctggagg aaccgccggg aaaccgtccg
     2281 ctgagcgacg agatggtgat cagcgcgcgc aagcgccttt tcgggtcgtg ggagatgaac
     2341 tggatggcat acaacttcgc tcaggatgtc gagctgccgg gatcgagtgg tacgacgctc
     2401 ccgtttttga tgtatccgca agcccagaca tccgagggga aactcgacag cctggatccc
     2461 gagaatttca aatacgagat cactgcgaga gaactttctt gatgcaagga gcaacgatga
     2521 acgtaagcac aactcgtgac tcagtccgcc acatgcctgc ggcgaatgga ctcgcggtga
     2581 ttcgagtaac gattggggcg atgttcatct gggtcttctt cgagaatctg gggaagggac
     2641 tctatacggc agccggttac gcgggattaa tccaatacta cgtccagcac agtcattcgc
     2701 cggcggcttg gaaggcgatc atggggctgg cggcgagcca cgccgcgatg gcggctcccc
     2761 tgcaagggct gactgaaatt tcgcttggcg tactcctgct catcggcctg ttcacgcggc
     2821 cggcggcttt tgtggcgttt cttttcctct cgagcctctg gctctcggag ttcggcacct
     2881 catggatctg ggaactgctg gttccggtga tggcatcgct ggggctggct atcggacgcg
     2941 cgggccgagc ctggggcatc gacggatggc tggagcggcg ctggccgaac gccccgtggt
     3001 ggttactcta agccacttcc agtgcgcgtc tccgtcatca ttcccacgca taatgaagcg
     3061 caggccatcg ggcgggtgct ggccgacctt cctgccagta tggtcaccga agtcctggtg
     3121 gtcgatagca actccacgga cgggacagct gaaattgcag cagcgatggg tgcacgtgtc
     3181 ctgcgcgaac cacgccgtgg atacggtcgc gcctgtttga ccggattagc aaataccgat
     3241 tcgcccgacg tcgtcgtatt cctcgacgga gactacagcg accgacccga tgagctttcg
     3301 cttctgctgg cgcctatcgc cgaaggtaaa gcggacatca cgctgggttc gcgccttgcg
     3361 aaaaagaacg ttcgcggtgc gctgccgtgg cactccgctt tcgggaaccg gctcgcggcc
     3421 ggcatgattc gggtgttgta tggccagaac atcagcgatt tggggccgtt tcgcgccgcg
     3481 cgtgccgatg tcctgcggca actcgcgctg gaagaggaga cttacggctg ggcggtggag
     3541 atgattctga agggttcgct cgcgggtttt cgcattgtcg aggttccggt cagctattat
     3601 ccgcgtatag gaaagtcgaa gatcagtggt accctgcggg gcacagccgg agccgcatgg
     3661 ttcatcctca gtcgtatcgt gagctattat ttccggcgcc gaaaggcatg acagcagaag
     3721 accagcgatc ttgtatggta tccagcgata ctctcccgca ttcgcgcagc aacgaccgca
     3781 cgctggtcat catggccaag gcgccgcggc ccggaatggt taaaacccgt ttagcgcgga
     3841 gtcttcccgt ggaagccgtc accgaccttt atcgctgcct attgggcgac accatggcgc
     3901 tggcgcactc gctgagcagc gggaaagttg ccatcatgtg ccctgcctct gacgtggaag
     3961 aactaacgca gttcgccgga gatacaaccg gtgtcgtggc gcagcaaggg gaaggccttg
     4021 cggcggggct gacgtcggtg tttgcgcagt tcgcgctcgg cggacattgc gtgattgcct
     4081 tcaatagcga cagcccgcat ctgccggttt cggtcctcga gcgggcattc gagatgctca
     4141 ccgtgcacga cgtggtggtc ggaccaaccc atgacggagg ctattacctg gtcggggcga
     4201 aggcccctca cgccacgcta ttcgatggcg acgggatggg aactaacagc gcgctcgacg
     4261 gccttctggc acgtgcgcgc aggctgcagc tttctgtcgg cttcgccgag cccttctacg
     4321 acattgatgt cgagggcgac ttgacccagc ttgctgagga cctgcggctt gctcctgcgc
     4381 gggcgccgcg aacggcagcg tggctgaaag agtggcaaca gcgagcgcca cagttacgga
     4441 atgccgctgg gggcctgtga agctcaatcc gtctcacagg ctgtacgggc tcggagctgt
     4501 tctgctggtg acgctgacca tctgctcccg caagttcggc ggcgagggtg aagcgtttcc
     4561 gatcattcca ctgttggtag ccggcgtagc ctatcttctg gccgttcgtg agttcttccg
     4621 catcccgaac ttcccaaagc gggtagttgt cattggcctg gtgttggcct cggtgtggca
     4681 cattcaattt ctacgaattc cgacgggttc cgatgacgat gtgcgtcgct atgtctggga
     4741 tggccgcctg cagcggctcg gttataaccc ttaccttgtg gttcccgatg accccgcgtt
     4801 tgccgccctg cacacagctg agacacaaac gctgaacaat ccagatctac ccagtatcta
     4861 tccaccgggc gcccagcttt tctttcgtgc cgttaccgcg attcatgagt ctgccttcgc
     4921 cctgcgactg gcatttgcgg tttgcgaact tgcgatggtt cttgtcttgc tcgatatctt
     4981 gcgtggtacc gggcaggcag cacactggat cctggctctt gcctggaacc cgctgctggc
     5041 gacagaagcg gtgggcagcg gtcacatcga tattttgggg gcgctgctgc tggttgtgtc
     5101 cttcgcggca ctaatacgcc gatggcgtgc ggtcgcggca gtgacgttcg ctctggcagt
     5161 agccgtgaaa tttcttccgg ttgtgctgct gccgctctac tggcgtcggg ttcgcattcg
     5221 cgacggtctg ctggccgtaa tcgtatttgt gctcttatac ctgccgtttc tcaaccacgg
     5281 gcgaattccc atcggctcgc tcggcactta catccatggc tttggtttta acgacccggt
     5341 ttttgccgtg ttgcggcgaa tggcggcccc gcagattctg gtgggattag ccgttttcgt
     5401 cgggcttctt attgcaacgt ggtacaggat tcgctcacgg gccttgtctg gtgacgcatt
     5461 tgcatggccg atggccgcct cgcttctgtg tgccccagcc gtgtttccct ggtatttgct
     5521 ttggctactg ccgtttgcgc gatccgcctc gactctcccg atcattatct ggacggtaag
     5581 cattatcccc acgtacatcg tctggcatct gcgcagcctg ggacatccgt ttgtgcttcc
     5641 gggctgggtg atgcttttgg agtacggagc tgttgggatc gccgctggag ccgtgctgat
     5701 acgccgaatc attcggccgg cagtgccgaa gtgcccggcc gactagctgc gttggctcgg
     5761 gttgcacaac caaagtcttg ctaacagacc tctcatattt tgaagccgcc gctcctcacc
     5821 gaacatccat tgctcaagcc accaaggggg atctccgtga cagattcgct ctgtaataca
     5881 gcggtacgtc tcttcctagt atttgggctc ctggttttcg ctgcaacttc ccaggcgcag
     5941 cagcataatc cgatactgga aaagctcgcc aaaacctacg gcattgattc gtgggataag
     6001 atcgaggcga tccgctacac ttggaatgga gaaatcaccg gactcttcaa attgagtcgc
     6061 acctggacct gggagcccaa gaccaacaag gtctcctacc agggcaagga tcaggacggc
     6121 aagccggtgg acgttaccta cctgcgctcc gaagtcagca gccagcccga cgccgtgaaa
     6181 aaggtcgact caggtttcat caacgataac tactggcttc tgttcccctt tcatgcttac
     6241 tgggacacca gcgccaccgt taccaatcaa cccatgcaga agctgccgat tggtaccggc
     6301 tcggctgagc tggtttcggt gaagtatccc tcggaagccg gcggttacac gcccggcgat
     6361 acgtggaatc tctacgtcgg aaaagacagc cgtgtcgagg tgctcgagtt ccaccatggc
     6421 gggaacgcca agccgagcct cgtcatagca acctggacag gctacaagaa ggcgggtcct
     6481 ctactgttct caaccgacca ccgcggcacg gctgacggca agcccctgca tattttcatg
     6541 acaaatgtgg ccgtcaagct ggtcgggtcc gataaatgga tgcctgcgca ataggcggcg
     6601 tgtctctatc gggcgattac tacggtagtg caggaactct tcagcttggg caggtcgcgc
     6661 ttggcggcca tgcgaatctc gaggcgcggc cgttctccgc gaatgattgg acaatagaaa
     6721 tcgggctggg cgagcgcccc ttgtagtcgc cttcgtccgg cacatccgcc gtggcacgat
     6781 tcgcggaact cgcacggtcg gcatgcgtcg ggaagttttc gcgcttgctc gaagggtgcg
     6841 gagtccagaa catcgggccc cattgagatc aattctgaca aaggcccccc tgatcctggc
     6901 cagtagacgc agggttgtgt cgtgcctctt ggtgttaccc gtattgtgct cactccacaa
     6961 ccccctcgga gcggcggaag tcccgccatt gcgcgaacca gtggctcgcc aatagcgatc
     7021 acgtcggtct tctcaaagag gcgccggaac ccttcccaat attcttcgta gctgagggcg
     7081 taaacgtccg atcgcacggc ttggtacaca ttcacccgca agggagcatc aaactgttta
     7141 gctacttgcg ccacatccac caaacgcaaa tagttcgcct tcatcatcac ggcgatgatg
     7201 gtcaccggaa tccgcagcct gagacaccgc tctgcctgct ggtggatgag cgcccaattg
     7261 ccacgcccgc gctgtgcatc ctgttccgat tccgtcgggt aatcgaggga gaactcgatg
     7321 tcatgaaatg cacgcagttc actgtcttcc aaaacggcaa tgctgtgacc attagaggtg
     7381 atggtgagct tgaccggctg cgttcgcaag taggccagta ttgccttgaa gtcgggatgc
     7441 atgccgttct cgccggtgcc gagattcacc gaccgcacgg ccacgcgttc catgacttcc
     7501 ttcacctgat ccagggagag acgatcggtg cgagttggat cgcggtagca gaaggcgcag
     7561 gcaagattac actcattggt aaggccgagc ccgagtgcaa taccggcacc aggtgtcatg
     7621 cgaggtccgg acgatgaaga ctgggctatc aggcgtctca tgcatctttc tttctaaccg
     7681 gaccagcgcg atgtgccaag caatgcgtgt agcgggcgac tgcggcgaac tccggtcgcg
     7741 tggccagctt gcgctctagt cccagaatct gctcgtactc gtcatttcgg gataccctcg
     7801 gaggcaagta atccgatatg acccgtacgc cacgctcggc aattaccgtg agcgccgatt
     7861 caaggagcat ggcttgcagg ccctgcggcg taaatagccg cactggtcct ccgtacagag
     7921 actcatgtcc ccacgtagcg gtgagattat gctccgtcgc gtccaagtcg ccgtcttgca
     7981 ttgcggcctt taacacctcc cccgcctggt tacgcaccaa taacgagatg acgctagacg
     8041 gatctcgcaa gacgcgagcc gcactccgca aaacagcgca cgggtcgtga acatattcaa
     8101 ggatattgtg acagagaatt acgtcaaacg actcggggtc gaacaggttt gccaattggc
     8161 tggcatcgcc gtgcatcagc gcgatgctct ttgtaatccc tgcttcctct gctgcgcgct
     8221 tcgcgaaatc cagcataggt acagacgcat ccagcaatgt gacatgaaga ccaagtttcg
     8281 ccagacgcag cgcgatggcg ccggtaccac atccaatatc tagagcgagc aaagactgtg
     8341 ttgctcgggg gagaaagtcc tgcaaattgg caaaagtgag gtcgaggcgc aatcggccct
     8401 cgggcgtcgc aaggtaggcg gcatacttcg ctgtcccggt ccggaaccgt tcgaagtcgt
     8461 ctttggtggt catttccttc atgcgccact gaggcgaagt cggcccgtac tagtagcagg
     8521 cgagtacgag agcgtatacc gaaaggatta ggaatgacaa gtcggccggt gtttacgaat
     8581 gctttccttt catctggccg tataacgccc aaaccggaga ttgaccgcct tacggaaggg
     8641 ttgcccccat ccacgaactg gctggggcac cgtctaacgt gccggaggcg gctctcccat
     8701 tcttctcgct gccattcccg caacgggtgc ttcccctaaa gacacggagt acactctccg
     8761 aaagttcaac taagatcggg accgggcctg cgcgatgatg gctgtgagct tgtcaggaac
     8821 cgaggggcac atgagatttc tggtgctgtt tctgctttcg atcacgagcg gcttgacgtt
     8881 cgcgcaagac tatccgacta agcccgtgcg catcatcgag ccgttcgggg ccggcggtgg
     8941 gcctgacctg gtagctcgcg ccatcagccc gaagctctca gagctgtggg gccagccagt
     9001 gacagtcgag aaccaccccg gcaaaggaag tacggaagcc cctgccctcg ttgcaaagtc
     9061 gccacccgac ggttacacgt tgctggtgaa caccagcgct caggcgtaca gcgccgcgct
     9121 cgtgaagacc ctaccgtatg acccgctcaa ggatttcatt cctgtcgctc cgcttaccag
     9181 ccagccctac gtgctggttg cgggaaagtc gtctggcatt acctctgttg gcgagctgat
     9241 agcggcggca agggcgaagc ccggtgagct gaagtttggc tccacaggcg tgggcactgg
     9301 cacgcacttg gggattgaga agttcaatct agaggcaggc attcgggctg cacacgtgcc
     9361 agcgcaaccg ggcgaggcga tcgctgacgt gatcgccaac gcagtcgcag gacgaactac
     9421 ctacatcatg gcacctatcc agcttgctct ggtcgatatt cgtgcgggca atctccgtgc
     9481 cctaggcgtg acgactaaga agcgctcgtc cttactgccc gaggttccga ccatctcgga
     9541 ggctggcgtc cccggcttta accatccaat ttggtatggg gtgtgggtgc ctgctggcac
     9601 tcctgccgaa gtggtggaca aactagcaaa agacatcgca cgcgcgctgg aagcgcctga
     9661 tgtgcgcgac cgacttgcaa aacacggtgc cgatccgatg agcatgacgc agcctgagtt
     9721 cgcgcgcttt gtcgtgagcg agagtgaaaa cgcggcgcgc atcgccaaag ccgttgggat
     9781 caaacctcag taaagtgtga gttgcacatt acgaagctct gctaaaggaa cgatcgctcc
     9841 gcttagatcc aggtgtcgaa agttgaaaag ctctagttcc acttgctctc aactctagct
     9901 ggataacgag caaaccggaa attggatgtc ctcagatagc gagtgtgaac agatgggatg
     9961 agaaggccgg tctcccggcg ggatgctgta cgctcccaag ttatcaa
//
LOCUS       DASRKK010000102         4717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_788168, whole genome shotgun sequence.
ACCESSION   DASRKK010000102 DASRKK010000000
VERSION     DASRKK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788168"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFC15_02255"
     CDS             <1..1050
                     /locus_tag="VFC15_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZM09013.1"
                     /translation="TRKGFGRSLQNNGDDERVVCLGLDISGSITISDFYSGKPERKNR
                     WISMGIAEQSATAAAAGLAREGKLPVFGTYGTFAAARNLDQIRVSICYGNFNVLIAGA
                     HGGVSVGPDGATHQALEDLFAMCGLPNMSVVVPCDAIETRKATDYLLLQHVGPKYIRF
                     AREATPIVTRENTPFVFGVANVSRLRREAPEMIDAFETVLAPNYENEHEDLSIIACGP
                     MVPEAMRAAWILKQEFGYETRVVNMHTMKPIDETAIVRAAKETGVVVTAEEHQIGALA
                     WRVSGVITESPELLGTPVITGAIGVKDRFGDSGAPWELIKEFEVSAEHIAQKAVELIA
                     IARMKKEEDRFQLAH"
     gene            1295..2080
                     /locus_tag="VFC15_02260"
     CDS             1295..2080
                     /locus_tag="VFC15_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM09014.1"
                     /translation="MRAQRYLLIPLLLAVAVGLHAQTVPYTIKVRDLRTKLAVHAKIQ
                     FEGPGSVSMETDGTGKLNVALPPGKYDEAISAPGYKTMKVSITIRPGGMPESIVMLEP
                     IKKAEDQTYRELEAQLRPGYTLVEGTALDEDGQPVADVKVRLEGKSIATTETTTGSKG
                     IYSFLIPTPPTTPIGPADSIPGTGTVIAEKPAYKTLVHDGVPLSDNSYWDVLLQMERG
                     SGTLRFDDMPVPMRGDDAIHDDEDDNAKPQEPAGGTLRLNQKR"
     gene            complement(3982..>4717)
                     /locus_tag="VFC15_02265"
     CDS             complement(3982..>4717)
                     /locus_tag="VFC15_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM09015.1"
                     /translation="TSAIASKKGLFEVADRGTLFLDEIGTMNLETQSKILRVLQDRKF
                     MHLGGVSEIQVDVRIIAATNVNLQQQVKDGKFRDDLFYRLNVITIDLPPLRQRKNDIP
                     MLAEYFIAKFAEENERLPLRMTPEAMRPLLEYDWPGNVRELENVIERAVVLATGPNLS
                     VDLLPDNVIGRGTKFAMMEHRPDASLFEIIEDCERRVISDMLEKCGWNQTEAADHFRI
                     PLSTLNQKIKRLNIEIKKKGRENGNS"
BASE COUNT         1068 a         1297 c         1364 g          988 t
ORIGIN      
        1 acgcgcaaag gcttcgggcg atcgctgcaa aacaacggcg atgacgagcg ggtagtctgt
       61 ctcggcctcg acatttccgg ttcgatcacc atcagcgact tctattctgg caaaccggag
      121 cgcaagaatc gctggatcag catgggcatt gcggaacagt cggcaaccgc tgccgccgcc
      181 ggactggcgc gggaagggaa gttgccggtc ttcgggacct acggaacgtt tgccgcggcc
      241 cgcaatctgg accagatccg ggtttccatc tgctacggaa atttcaatgt attgatcgcc
      301 ggagctcacg gcggagtttc ggttggaccc gatggcgcca cgcaccaggc gctggaagat
      361 ttgttcgcca tgtgcggact gccgaacatg tcagtcgtgg tgccctgcga cgccatcgag
      421 acgcgcaagg cgaccgacta tctgctgctc cagcatgtcg ggcccaagta catccgcttc
      481 gcgcgggaag ccactccgat cgtcactcgc gaaaacacgc catttgtatt tggagtggcg
      541 aacgtgagcc gtctccgccg cgaagcgccg gaaatgatcg atgcgttcga aaccgtgctt
      601 gcccccaact acgaaaacga acacgaagac ctgagcataa tcgcttgcgg gccgatggtg
      661 cccgaggcca tgcgtgcggc ttggatactg aagcaggagt ttggctacga gactcgcgtg
      721 gtcaatatgc acaccatgaa gccaattgat gaaacggcca ttgtgcgggc ggccaaggag
      781 acgggcgtag ttgtcaccgc cgaggagcac cagattggcg ccctggcgtg gcgcgtgagc
      841 ggtgtaatca ccgaaagtcc cgagttgttg ggcacgccgg tgattacggg cgccatcggc
      901 gtaaaggacc ggttcggcga ttccggtgcg ccgtgggaac tgatcaagga gttcgaagtc
      961 agtgccgagc acatcgcgca gaaggcagtg gagttgatcg ccattgccag gatgaagaag
     1021 gaagaagatc gcttccaact ggcgcactag aacggggcac ccatgtttga acagacctcg
     1081 tcaacaagag ctttggccgg gccacccgcc cataaagtta agtaacaaag gcccgcggcg
     1141 aggtaacacg ccgaagctaa ccagggccca ttgagtctgt agtttcattt cggcattgat
     1201 tcccaaaata aagccgttcc tttgttgatt ttcgttgctt tgacccgaat gccgtggtac
     1261 gagacggcta gttgtctttc aggaggacac ttcgatgcga gctcagagat acctcttaat
     1321 cccattgctg ttggctgttg ctgtaggtct ccatgcacag actgtcccgt acaccatcaa
     1381 ggtgagggac ctgcgcacca agcttgccgt ccacgcgaag attcaattcg aagggccagg
     1441 gtcggtctcc atggaaacag acgggaccgg aaaactgaac gtcgctttac cgcccggaaa
     1501 atatgacgag gcgatttctg cgccgggata caaaacgatg aaagtgtcga tcacgatccg
     1561 accgggggga atgccggagt cgattgtgat gctggagcca atcaagaagg ccgaagatca
     1621 aacctatcgc gaactggagg cgcaactccg accgggttac acgttggtgg aaggtacagc
     1681 cttggatgag gacggccagc cggtagcgga cgttaaagtt cgcctcgaag gtaagtcgat
     1741 cgcgacgact gaaacgacta ccgggagcaa ggggatctac tcgttcctga ttcccacccc
     1801 acccacgact cccatcggcc ccgccgatag tattccagga accggaaccg tgatcgctga
     1861 aaaaccagcg tataagacct tggtccatga cggcgtaccg ctcagtgaca atagttactg
     1921 ggacgtactg ctccaaatgg agcgcggctc cgggactctg agattcgacg acatgccggt
     1981 tccaatgagg ggcgacgacg cgatacacga cgacgaggac gataatgcca agccgcagga
     2041 gccagccggc gggaccctac gcctaaacca gaagcgatga tcgggacgtt ccctctccct
     2101 gagacgataa cggttggcac agtctgtcct aaattggatt gtcaaagtaa cgatcctcag
     2161 ggaaagtgct atttcagtga ctgcactaaa cacccggacg gctgcccgct atgcgctaag
     2221 aaatacggcc catgcaccgg ctcggtcatc actactccgc tggaggctta tgtccaatat
     2281 ggcctgcaac aagaatggtt aaaagagtgg tttcctcctt acgatgcact agaggccgga
     2341 gcggtagctt atcggacttt cggcacatac ttcattgaac acccagggca ttcaggctca
     2401 tctagctatg atattcggga tgacacatgt aaccaaaagt atgtagacat taaaaacccc
     2461 gcgcacgaca cttatgcagc cgcgactaga accatcggta tcgcaatgtc acaagatggt
     2521 gtgaatgcat acaactcaga gtactccagg aacacgaatc accagccccc tgaccctaac
     2581 catgacccac ccaagtggcc tggatgtatg gacggctact cgggaaatgg agcaggttgg
     2641 ccgtgcatgc aagatcccat tgccgctgga acgacgcacg cgggtcacgg tcgcggaatg
     2701 agccagcagg gttcatggtg gtgggcaagg ggccagagct atcagggaca gaccaccccg
     2761 gcaccagggt ggcaatgcat cttggaccac tactacaacg ataatggcaa cagtaccgga
     2821 gcgggaggcc aatttacata ccggtattcc ttcctctacg gtccgggcgg cgacggacaa
     2881 attgtgttcg ctccgaccag tatcattccc agcaggcgcg atgtccgcaa cccagcggat
     2941 ttgtgcgagg gctcgtggcc gagcgacatc tttacgatgc aattagatgg aagcaacctc
     3001 gcgcagatta cccagaacca gtgctacggg cgacctgtgt ggtcgcctac gcagactcaa
     3061 atctcattta ccgccctacc gtttttggtg aacataatga gtgcaacggg gggtctcccc
     3121 acgtacttgg ggtatgggtt cgacccagac tggtcacgcg agaacgtgat tgcgtttaat
     3181 aatctgtcag ggctgtatag ggtgaatcct gatggttcgg gggcttttca gataaattta
     3241 gatcaaaatg cgggagacgc attttggtct cccgacggaa acacgatcgt ttacgatacc
     3301 tgctcagctc ccggttgtga ttcaattcag atagccctga tgaacgccaa tgggacgggc
     3361 cagccaatct tcctcacttc tgggagcaat aacaccgagc cagcatggtc gccggacggc
     3421 aaaaatacag tcttcatatc cgacaggagc ggctcgggtg agatctgggt catgaactcc
     3481 gatggctcag gaaacccggt tcaaaagaca ttctcctcac catatcccgt tgcctttccg
     3541 agatggtctc aggacggcca gtatattgtg tggctcacgg taattggaga cggcaccgca
     3601 cgtcttaata tgatgaatgc ttcgggtgga gtcccggtca cctacggacc cactcttcaa
     3661 gggggtgccg gtccatcgat ggacacgacg cgctgtcgga aattcgatgc gctgtaagaa
     3721 ccgaaaccag cctggtgctc tagggtcgcg tccgtctttt gggcgctaga ttagaagtcg
     3781 tgccctgata caaatctggc ctgcgactat ttttgaaaca cgttctaggt gccgaacatt
     3841 accgatggcg gaatagcctt gccgtgccga tctggctgca gggtcgttat gatctcgcca
     3901 acattgacgg ttatcgcttc tcccggctgc agtgtggttt ggttgggtag gattcgaacg
     3961 tttcacgaga cctgctaagt gctagctatt cccattctcc cggcccttct tcttgatctc
     4021 gatattcagg cgcttgatct tctggttcag ggtggagagc ggaatgcgga agtggtcggc
     4081 agcttcggtc tggttccagc cgcatttttc cagcatatcg ctgatgacgc ggcgctcgca
     4141 gtcttcaatg atttcgaaca gcgaggcgtc ggggcggtgc tccatcatgg cgaacttcgt
     4201 gccccggccg atgacattgt ccggcagcag gtctacggag aggttcggcc cggtcgccag
     4261 cactaccgcg cgctcgatca cgttttccaa ttcgcgcaca ttgcccggcc agtcgtactc
     4321 cagcagcgga cgcatcgctt cgggagtcat gcgcagcggg agccgctcat tctcttcggc
     4381 aaacttggcg atgaagtact ccgcgagcat cggaatgtca tttttgcgct gccgcaacgg
     4441 aggcaggtca atggtgatca cgttaagacg gtagaacagg tcgtcgcgga atttcccgtc
     4501 cttcacttgt tgctggagat tgacgttggt ggcggcgata atgcgcacat ccacctgtat
     4561 ctcgctgacg ccgccgaggt gcatgaactt gcggtcctgc agcacgcgca ggattttgga
     4621 ctgcgtctcc aggttcatgg tgccaatctc gtcgaggaac agcgtcccgc gatcggccac
     4681 ctcgaacagg cctttcttgc tggcgatggc gctggtg
//
LOCUS       DASRKK010000103         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_104120, whole genome shotgun sequence.
ACCESSION   DASRKK010000103 DASRKK010000000
VERSION     DASRKK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104120"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFC15_02270"
     CDS             complement(<1..417)
                     /locus_tag="VFC15_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09016.1"
                     /translation="MRKTENIFWLGIKELRSFLHDFVLLGLVIYAFSFAVIMQARSNS
                     QELNNASIGIVDEDHSELSYRIAHAFLPPYFQPPQLIAERDIVPLMNRGRYTFVVDIP
                     PNFQRDVLGGRQPVLQINVDATAMVQAGLGSGYAQQI"
     gene            complement(432..>2981)
                     /gene="rbbA"
                     /locus_tag="VFC15_02275"
     CDS             complement(432..>2981)
                     /gene="rbbA"
                     /locus_tag="VFC15_02275"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HZM09017.1"
                     /translation="VFVLNGDIADSAHRAAICPRIAYMPQGLGKNLYADLTVRENIEF
                     FGRLFGQSRSERDSRISELLQSTGLAPFSDRPAKKLSGGMRQKLGLCCCLIHDPDLLI
                     LDEPTTGVDPLSRRQFWELIDRMRSRRRGMSVVVATAYMEEAERFDWLAAMNAGKVLA
                     SGSPAELKASTGGATVEDAFIALLPEQQRGGHHALHIPPRRIFDVAPVISARDLTCRF
                     GDFTAVNRVSFNIERGEIFGFVGSNGCGKTTTMKMLTGLLPPSEGEALLFGKPLDARD
                     MSSRYRVGYMSQSFSLYTELTVQQNLDLHARLFHLPVQKAKARITELVAQFGLEDYLD
                     QRTLDLPLGIRQRLSLAVAIVHQPEILILDEPTSGVDPLARDRFWELLIDLSRNQGVT
                     IFVSTHFMNEAERCDRISLMDAGRVLATDTPANLIKARGVATLEDAFISYLEEATGSR
                     ASGVEDFAPSGEIGNPVRPPTPARPLFSLQRLFAYTIRETLELLRDPIRLGFGLFGTA
                     LLMLVLGFGISTDVNNLSFAVLDRDQSHESRAYLEELRGSTYFVEKSPLADYAELDKR
                     LKSGDIKAAIEIPPAFGRDIKRGRPAWVSAWVDGAMPFRAETIRGYLQGMHQLYLADP
                     AVKTTVPAARPPADIEVRFKYNQDFDSINAMVPSSMSMLLALFPAILMALAIVREKEL
                     GSITNLYVTPVTRIEFLLGKQLPYIAVAMVNFALMFLMALFVFEVPFKGSFPTLLLGA
                     LIYVTTTTAYGMLISAFTRTQIAALFGTAILTVLPATQFAGLMTPVSSLSGMAQIMGR
                     CFPMTYFVPISVGTFTKGLGFADLSANLAALVVFIPVLTLLSRLLLRKQER"
BASE COUNT          684 a          844 c          888 g          565 t
ORIGIN      
        1 gatctgttgt gcatagccgg acccaagacc cgcctgcacc atcgcagtcg catcgacgtt
       61 gatctggagc acgggttggc gtccacccag aacatcccgt tggaagtttg gagggatgtc
      121 gactacgaag gtgtatcttc cgcggttcat caagggaacg atgtcccgct cggcgatcaa
      181 ctgcggcggc tggaaataag gtggcaagaa tgcatgtgcg attcggtacg agagctcgga
      241 atggtcctcg tcgacgattc cgatcgacgc gttattcagc tcctgcgaat tgctccgcgc
      301 ctgcatgatc acggcaaagg agaatgcata aatcaccagc cccaaaagca caaaatcgtg
      361 caggaaactg cgcaattcct tgatgccgag ccaaaagatg ttttcggtct tacgcatcgg
      421 cccaactcct cctagcgttc ttgcttgcgc agcagcagac ggctcagcag ggtcaggacc
      481 gggataaaaa ctaccaacgc tgccaggttc gcgctaaggt cagcaaagcc aagcccctta
      541 gtgaaagtgc cgacgctgat cggcacgaag taggtcatcg ggaagcaacg acccatgatc
      601 tgagccatcc cggacagaga ggaaacaggg gtcatcaagc cggcaaactg cgttgcgggc
      661 aggactgtta aaatagcagt tccgaaaagc gcggcgatct gcgtgcgcgt gaatgccgaa
      721 attaacatgc cgtaggcagt cgtcgtagtg acatagatca gcgcccccag aagcagcgtc
      781 ggaaagctgc ccttgaacgg aacctcgaag acgaacaacg ccatcagaaa catcaaagca
      841 aaattgacca ttgctaccgc gatgtaaggg agttgcttgc caagcaaaaa ttcgatccgg
      901 gtaacggggg tcacgtaaag gttagtaatc gatccgagct ccttttcccg aacaatggca
      961 agtgccatca ggatcgcagg aaacagcgcg agcagcatgg acatgcttga aggcaccatc
     1021 gcattgatgc tgtcgaaatc ctgattgtac ttgaacctga cttcgatatc ggcaggcggc
     1081 cttgcggcgg gtaccgtcgt cttcacggcc ggatccgcta ggtacaactg atgcatgcct
     1141 tgcaggtagc cgcggatggt ctctgcccgg aatggcattg cgccatccac ccacgcgctg
     1201 acccaggccg gacgtccgcg cttgatgtcg cggccgaagg cgggcggaat ttcaatggca
     1261 gccttgatgt ccccgctctt gagacgcttg tcgagctcgg catagtcggc gagcggcgac
     1321 ttctccacaa aataagtcga gcccctgagc tcctcgagat aggcgcggct ctcatggctc
     1381 tggtcccgat caagcaccgc aaatgagaga ttattgacat cggtcgaaat tccgaatcct
     1441 aacacgagca tcagcagtgc cgtgccgaac aagccgaacc cgagccgaat cgggtcgcgc
     1501 aggagctcca gggtctcacg gatcgtgtag gcaaacaggc gctgcaggct gaacaaggga
     1561 cgcgctgggg ttggtggtcg taccggatta ccgatttcgc ctgacggggc gaagtcctcg
     1621 accccggaag cgcgtgatcc ggttgcctcc tcgagataac tgatgaaggc gtcctcgagc
     1681 gtggcgacgc cacgggcctt gatgaggttc gccggcgtgt cggtcgcgag cacgcgtccc
     1741 gcatccatga gcgatatccg atcgcaacgc tcggcctcgt tcatgaaatg ggtcgagacg
     1801 aaaatggtta ctccctgatt gcgcgaaaga tcgatcagca attcccagaa gcggtctcgt
     1861 gccaagggat cgacgcccga ggtcggctcg tcaaggatga gtatctcggg ctggtgaacg
     1921 atggcgacgg ccagcgagag ccgttggcgg atccctagcg gcaaatccaa ggtgcgttga
     1981 tcgagataat cctcaaggcc aaactgtgcg accaactcgg taatacgtgc cttcgccttc
     2041 tgaaccggca ggtggaaaag gcgtgcatga agatcgaggt tctgttggac cgtgagttcg
     2101 gtatagagag aaaacgactg tgacatgtaa ccgacgcggt agcgcgaact catgtcgcgt
     2161 gcatcgaggg gttttccgaa caagagcgcc tcgccttcgc ttggcggcag caagccggtt
     2221 agcattttca tggtcgtggt cttgccgcag ccgttcgagc caacgaagcc gaagatctca
     2281 ccccgttcaa tgttgaagct cacgcggttg actgctgtga aatcaccgaa acgacaggtg
     2341 aggtcgcggg cgctgatgac aggcgcgacg tcgaagatac gacgcggcgg tatatgcagc
     2401 gcgtggtgac caccgcgctg ctgttccgga agcagcgcaa tgaatgcatc ctctaccgtc
     2461 gccccgccag tactggcctt gagctcggcc ggggatccgc ttgccagtac cttcccagcg
     2521 ttcatcgccg ccagccagtc gaaccgctcc gcttcttcca tataggccgt cgcaaccacc
     2581 acgctcatac cgcgacggcg cgagcgcata cggtcgatca gctcccagaa ttggcggcgc
     2641 gacagcgggt cgacgccggt ggttggctcg tcaaggatca ggagatccgg atcgtggata
     2701 aggcagcaac aaagcccaag cttttgtcgc atgccgcccg acaatttctt cgccggacgg
     2761 tcggaaaagg gtgcgagccc ggtactctgg agtagctcag agattctcga gtcccgttcg
     2821 gaacgggact gcccgaatag gcggccaaaa aactctatgt tctcgcggac ggtgagatcc
     2881 gcataaaggt tcttgcccaa tccttgcggc atataagcga tgcgcgggca gatcgcggct
     2941 cgatgcgccg aatccgctat gtcgccattg aggacgaaaa c
//
LOCUS       DASRKK010000104         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_592662, whole genome shotgun sequence.
ACCESSION   DASRKK010000104 DASRKK010000000
VERSION     DASRKK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592662"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..937)
                     /locus_tag="VFC15_02280"
     CDS             complement(278..937)
                     /locus_tag="VFC15_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZM09018.1"
                     /translation="MALLDRVATLVRANLNDLVDRAENPEKMLKQVILDMENQFIQVK
                     TQVAIALADLHLLKKKKQENAEKHAEWMRKAELAVDKKDDELARAALERFVSFQQLTE
                     SFEEQISDQDAQVESLKSALKKLELKLAEARAKADLLIAQHRRSRAVNRAADAQQSSG
                     YESLTFDRMRSKVAREEALGKAKGELLGDDLDGRFHTLEREQKIDALLEEIKAKRRLS
                     A"
     gene            complement(1082..2551)
                     /locus_tag="VFC15_02285"
     CDS             complement(1082..2551)
                     /locus_tag="VFC15_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZM09019.1"
                     /translation="MGFHIPTEALIVAWTAVAAVVLVIAVFARLYRKAGPNEVLVVYG
                     FRGTRVVKGHGTVIFPMVETYRELSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVK
                     SDPESVLTASEQFLTKPPQDREALIRLVMEGHLRGIIGQLTVEEIVKQPEMVADRMRS
                     TCADDMNKMGLEVISFTIKEVRDKNEYIANMGRPDVARIKRDADVATAEAERDTAIRR
                     AIALRESAIAKAQADQERVVAETASLARQAEAQRDLEVKKATYNELVKRQQAQADKAY
                     EIQTNIMLQQVTAEAVKVQQVEKEQQVKVQEAEILRREKELIATVLKGAEIDRKRIET
                     LAEAERQRLTVEAEGHASAIRAQGEAEAEIIFKKGDAEARAMNLKAAAYQEYNQAAVY
                     DKLITGLPEVVKALSSPLNNVDKITIVSTGNGSSAGMHKITGDITEMAAQVPALFEAL
                     SGMKMSDLLGKIRPIGDKSAGELPPAGGDGNSTVAGSSK"
     gene            complement(2569..3159)
                     /locus_tag="VFC15_02290"
     CDS             complement(2569..3159)
                     /locus_tag="VFC15_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09020.1"
                     /translation="MTWAMFYLVCFLVGVTLSVLSFFGGSFHLPHFHLHVPHVHVPHG
                     GAHGGSGGEMPFLNFGTITAFLAWFGGAGYLLTRYSSLVVAGVLMLAVVAGIVGATIV
                     FWFVAKLLLKHDRELDPADYDRVGVLARISSPIREGGTGEIIFSQEGTRNTCGARSEN
                     GEALARGTEVIVTRYERGIAYVRRWEELAEKDVTSS"
     gene            complement(3156..3680)
                     /locus_tag="VFC15_02295"
     CDS             complement(3156..3680)
                     /locus_tag="VFC15_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09021.1"
                     /translation="MKLGEKLRYLRLVEGTLRGMEREMTQQDLAVALKKELGSGLSQS
                     YLSQIESGARPHLTNASRMLLARFFKVHPGYLVDDPEGYHTELTSDLRAREDRLDLWL
                     INGAEQFARDPELSQALVDVAKYKDSRKCLVLLGAILEAPELVERLTEVLKPNPREPV
                     HRGAGKKRLAGAKL"
BASE COUNT          844 a         1441 c         1247 g         1050 t
ORIGIN      
        1 caacacgatt gaataggcat aatcgcctcc ccacgggcgg ctcccttcaa accgctgtat
       61 cggacgcgcc ccgccggatc tcccaggctc acctgcacac ttaccctcgg gcgcatccga
      121 tacagcacta aggtttctgg atcgtgatcg aagcgcccca gcgcaacgtg agatgattca
      181 tgttctggga acctgggctc tccgattgcc gaccatccag aattgccaga accggcattc
      241 tcttgataac ccgcaacgac cggacactat tcagccgcta ggcacttagg cgtctttttg
      301 ccttaatctc ctccaacagt gcatcgattt tctgctcccg ttccagcgtg tgaaaccggc
      361 catcaaggtc gtcgcccaac agttcaccct tggctttacc cagagcctcc tcgcgggcga
      421 ccttggagcg catgcggtcg aaggtgagac tttcgtagcc tgaactttgc tgggcgtctg
      481 cggcccgatt cactgctcgc gagcggcgat gctgcgcgat cagcaggtcc gccttggctc
      541 gagcctcggc aagcttgagc tctaactttt tcaaggcaga tttgagcgac tcgacctgcg
      601 cgtcctggtc ggagatctgc tcttcaaagc tttccgtgag ctgctggaag ctcacgaacc
      661 gctccaaggc tgcacgcgcc aattcgtcat ctttcttgtc gacagcgagt tcggctttgc
      721 gcatccattc tgcgtgcttt tcggcgtttt cttgcttctt tttcttcaag agatgaaggt
      781 cggcaagcgc gatcgccacc tgggtcttca cctgaatgaa ctggttctcc atgtccagga
      841 tgacctgctt caacatcttc tcgggattct ccgcgcgatc cacgagatcg ttgaggttgg
      901 cgcggaccaa cgttgcgact cgatcgagta atgccataac tgctccttgc tgaatttcag
      961 gatgtgcgtt cagcgcgcat caagctcttt cccctggaac ctgggcttcc caccctgccg
     1021 gcttggttcg cggcggactg ctgccgacgc ccttgcgaca gaatgggcac gttctgtcat
     1081 ttcacttact gctaccggct accgtggagt tgccgtcgcc tcctgctggc ggtaactcac
     1141 ctgccgactt atcgccaatg ggccgtatct ttccaagcag gtcagacatc ttcatgcccg
     1201 agagggcctc gaacagcgcc ggcacctgcg ccgccatctc ggtaatgtcg ccggtgatct
     1261 tgtgcatgcc ggcggaagag ccgttgccag ttgaaacgat ggtgatcttg tcgacattgt
     1321 tcagcgggct ggacagcgct ttcaccacct ccggcaagcc ggtaatgagt ttgtcgtata
     1381 ccgcggcctg gttgtactcc tggtaggcgg cggctttcag gttcatcgcc cgcgcttcag
     1441 cgtcgccctt cttgaagatg atttcggctt ccgcctcgcc ctgggcgcga attgcgctgg
     1501 catgaccttc cgcctccacc gtcaaacgct gccgttcggc ttcggccagg gtttcgatcc
     1561 gcttacgatc gatttcggcc cccttcaaca cggtggcgat gagttccttc tcgcgacgca
     1621 ggatctcggc ctcttgcacc ttcacctgct gttccttctc aacttgctgc actttcacgg
     1681 cctcggcggt cacctgctgc agcatgatgt tggtttgtat ctcataggcc ttgtcggctt
     1741 gcgcctgctg gcgttttacc aactcgttgt aggttgcctt ctttacttcg agatcgcgtt
     1801 gcgcctcagc ttgccgcgcc agggaagccg tttcagccac gacgcgctcc tggtcagctt
     1861 gcgctttggc gattgccgat tcccgcagcg caatggcgcg ccggatggcg gtgtcgcgtt
     1921 ccgcttcggc ggtcgcgacg tccgcgtcgc gcttaatgcg cgccacgtcg gggcgcccca
     1981 tgttggcgat gtactcattc ttgtcacgca cttccttgat ggtgaacgag atcacctcga
     2041 gccccatctt gttcatgtca tcggcgcatg tggagcgcat ccgatcggcg accatctctg
     2101 gttgctttac gatctcttcc accgtgagct gcccgatgat gccgcgcagg tggccttcca
     2161 tcaccagccg gatcaaagct tcccggtctt gcggcggctt ggtcagaaac tgctctgacg
     2221 cggtcaacac cgattccggg tcggacttca ctttgatttg cgccacggct tctaccgtga
     2281 cggcaacgcc ctgcttggtg tagaggtcct gctgcggcgc cacgtcgaag gacatcaact
     2341 ccagcgatag ctcgcggtag gtctcaacca tggggaagat gaccgtaccg tgtcctttca
     2401 ctacgcgggt gccgcgaaat ccatacacca ccaggacctc gttgggcccg gccttgcggt
     2461 acagcctggc aaataccgcg atgaccagca ccacagccgc taccgcggtc caggcaacaa
     2521 ttaacgcttc cgttggaata tgaaacccca tacagcctcc ttgaatattt agctgcttgt
     2581 tacgtccttc tcagccaact cttcccagcg ccgcacatag gcgatgccgc gttcgtaccg
     2641 cgtgacgatc acctcggtgc cgcgtgccag ggcctcgccg ttttcgctac gtgcgccaca
     2701 ggtgttccgc gtaccttctt gcgaaaagat gatctcgccg gtacccccct cacggatcgg
     2761 gctgctgatg cgcgccagga ctccgacgcg gtcgtaatcc gccggatcga gttcgcgatc
     2821 gtgcttgagc agcagcttgg ccacaaacca gaagacaatc gtcgcgccca cgattccggc
     2881 aacgacggcc agcatcaaca ctcccgcgac cacgagcgag gagtagcgcg tcagcaaata
     2941 tccggctccg ccaaaccagg ccaggaatgc cgtgatcgtc ccgaagttca ggaacggcat
     3001 ctcgccgccg gagccgccgt gtgcgcctcc gtgcggcacg tgaacatgcg gcacatggag
     3061 atggaagtgg ggcaggtgaa agcttccacc gaagaacgac aacacgctga gcgtcactcc
     3121 caccagaaaa cacacgagat agaacatcgc ccacgtcata gcttcgctcc cgccaatctc
     3181 ttttttccgg cgccgcgatg cactggctct ctcggattcg gcttcagaac ttccgtcaag
     3241 cgctccacta attccggcgc ttccagaatc gcgcccaaca gcaccaggca tttgcgcgag
     3301 tccttgtact tggccacgtc gaccagcgcc tggctcaact cgggatcgcg ggcgaactgc
     3361 tctgcgccat tgattagcca aaggtcaagc cggtcttccc gagcgcgcag gtcgctggtc
     3421 agctccgtgt gatagccctc gggatcgtca accaggtatc ccggatgcac cttgaagaac
     3481 cgcgctagca gcatgcgaga cgcgttggtc aggtgcggcc gcgctccact ctcaatctgc
     3541 gacagataag actggctcaa tccgctgccc agttccttct tgagcgccac ggcgaggtcc
     3601 tgctgcgtca tttctctttc catgccgcgc aacgtgcctt cgaccaggcg caggtaccgg
     3661 agtttctcgc cgagtttcat attacgatta gcaattataa tattgcaatt tgtaatgtca
     3721 aggactggat ttggtttgtt atcaggtaat tgggaagtaa ggctcggcag gtattccgtc
     3781 gctctaagcc gcggggatct tcgccctttt ggtgctcagc gccgcgagtt cttcgtaggc
     3841 ctattccctt tgatgtggca atccatcagc tccgtgaccg aggcaagggc gcacttgcag
     3901 cacgccttgc cgatccgaga ttgcatctac aatatgaacg aatcgtaagt tgtcggccga
     3961 tgcagttcgg agcgcgattc atcggcgctc tgaacagcga cccgcaccgg cggctggcgc
     4021 cgcggagttc cccagaaatc ctcttctggg aagttccacc agataggtaa cgagaaacgc
     4081 tcatcgcgac cgtattcgct gccggctcgc ttcgcttcga gatcacagcc tgtttgccga
     4141 cttcccgtta ccgatgctga gcttcggtgc tgcccaggta cccgtccggg gatccggcgt
     4201 ggtggagcca ggacccatgg ccgaaacaag ttcgctggag tgcgtcacga ttttcgcagt
     4261 ccctcggcag acacagacag cgggtcaaac cccatggtcg ctaagtacgt tgcctcactt
     4321 ggagcaggcg caagagttgt gcccacgaaa cgctcggttt ggggcggacg attgacaaca
     4381 tcgctctaat agatatatat atattagaca tatggcaaaa cgtacaagtt cgagcatgga
     4441 agtgcttctt ggcctgctga cgatcgcgcc catgtctggc tacgatctgg ggcaggccat
     4501 ccgcgaatcg gtgggctttt tctggaacga gagctatggg cagatctatc caaatctgaa
     4561 agctctagcg gctgaggggc tc
//
LOCUS       DASRKK010000105         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_397658, whole genome shotgun sequence.
ACCESSION   DASRKK010000105 DASRKK010000000
VERSION     DASRKK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397658"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFC15_02300"
     CDS             <1..225
                     /locus_tag="VFC15_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09022.1"
                     /translation="GGTRKPISYSMHGQIDTGLIAMTAAMPRLVNIEDDREAKFFSRQ
                     ALAKTAITAMTDFDYDQRKISGRTRDNGFV"
     gene            364..1224
                     /locus_tag="VFC15_02305"
     CDS             364..1224
                     /locus_tag="VFC15_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZM09023.1"
                     /translation="MGNTFKTALLLTAMTLLLLFLGQAFGGERGMMIALVFAVVMNFT
                     SYFFSDKIALAAYRAQPVTREQLPRAYEIVERLTQKIGIPMPKMYVIPTDSPNAFATG
                     RNPQHASVATTEGILNLLTDEELEGVLAHEIGHVRNRDILTSSVAATLAGAITLIARM
                     GYWASLFGGGGGRDDRDRGGGFVALLMLILAPIAATLIQLAVSRSREYEADASGAKYT
                     GNPYALASALKKLDADSKRIPMQATPSTAHLFIVAPLLGVDFSSLFSTHPPIAKRIER
                     LTGRPAELEP"
     gene            1714..2163
                     /locus_tag="VFC15_02310"
     CDS             1714..2163
                     /locus_tag="VFC15_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM09024.1"
                     /translation="MKSAAYRLPGKRAMAEMAWDGMCRSEPDERVWTGMKAPSNARQT
                     CSANPDVLVDSADWTAGRPEASLDTLEPGGASLNQISSEQAASVVAALASVTPEKPPA
                     GTKVPEPPTLALLGAGMIATSSLSRVTRQPKCKLRVVATRLGAVLQH"
     gene            2917..3141
                     /locus_tag="VFC15_02315"
     CDS             2917..3141
                     /locus_tag="VFC15_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09025.1"
                     /translation="MQVESDHTAEINQRERIWFFPDKFWQATKGMPQDEVSKLMAEVE
                     QYAEAKNVEALRKYPFVFVGDPYKDHNNAA"
     gene            complement(3301..>4608)
                     /locus_tag="VFC15_02320"
     CDS             complement(3301..>4608)
                     /locus_tag="VFC15_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZM09026.1"
                     /translation="FLSVDPDPAWRQMKKLGKGLFASLGDLRDTQVMMEWVGKLSAPD
                     DPLRQILFDSLQQKEEALKAVAKEAVSSFDRKRWLALNTHLADRATRVPLNGLVFQYL
                     ALERWHDAYELHRTALRNRSAVAFHQLRIGIKRFRYTVENFLPERQPLWSHDLRDLQD
                     ALGEVHDFDVLWAMVKTHAEVGLEERALWQQTISDERQKRIALYRKKMMGRESLWQKW
                     RAELPAGEALAKAALEKMRTWAAFLDPDDKHAQLVTTLALEIYDGLAREGILAESEMS
                     RQILEAAAVMQDVGRGKAGGHRKRGYRMIRNLKPPVGWSEEYLQCVAIVAHFHRGGLP
                     PTNHPIFAGLPAQRRGELMPLAGVLRLANALDELHDQRILSVKVERGNRVLILHAQGL
                     TSSISPFGEQLARARYLLETCIKMPIMIRPLPAHRKPTTPAAI"
BASE COUNT          945 a         1381 c         1336 g          946 t
ORIGIN      
        1 ggcggcacgc gcaagccaat ttcctattcg atgcatggcc agatcgatac cggactgatt
       61 gccatgaccg ccgccatgcc gcgcctggtg aacatcgagg acgatcgcga agccaagttc
      121 ttctcccgcc aggccttggc caagaccgcc atcacggcca tgacggattt cgattacgac
      181 cagcgcaaga tatcgggccg cacgcgcgac aacgggttcg tctaacccca atcaagtggg
      241 ctactaagcg gcatgggtag cattactcag ggcaaccaca cacgcgtttc tcgccgggac
      301 gggcgcgcaa ccgttttgcc gccgcatcat ctaaacgact gacccacaca cttggaaggc
      361 cctatgggaa acacctttaa gacggcgttg ctgctgacgg cgatgaccct tctcctgcta
      421 ttcctggggc aggcgttcgg cggcgagcgc ggcatgatga ttgctctggt attcgcggtc
      481 gtcatgaact tcacttcgta tttcttcagc gacaaaatcg cgctggcggc ctatcgcgcg
      541 caacccgtaa cccgcgagca gttgccgcgc gcctatgaaa ttgtcgagcg tctcacgcaa
      601 aagatcggca tccccatgcc gaagatgtac gtgataccca ccgactcgcc caacgccttc
      661 gccaccggac gcaatccgca acatgcttca gtggccacga ccgaaggcat cctgaacctg
      721 ctgaccgatg aggaactgga aggtgtgctg gcacacgaga tcggacacgt ccgcaatcgc
      781 gatatcctga ccagttcagt ggcggccact ctggcgggcg cgattacttt gattgcgcga
      841 atgggctatt gggcttcgct gttcggcgga ggcggcgggc gcgacgaccg tgatcgcggc
      901 ggtggcttcg tggccctgct gatgctcatc ctcgcgccga tcgcagcgac cttgattcag
      961 cttgcggtgt cgcgatcgcg ggaatatgag gccgatgcca gcggcgcgaa atacactggc
     1021 aatccctacg ctctcgccag cgccctcaag aaactggacg ccgattcgaa acggatcccc
     1081 atgcaggcga ccccttcgac ggcgcacctg ttcatcgtgg ctcccctgct cggagttgat
     1141 ttctcgagcc tgttctcgac ccatcccccg attgcgaaac gcatcgagcg actgacagga
     1201 agaccggcag agttggaacc gtaagcctgc gcacaatttg actcagactc cgggtgaaaa
     1261 gagtttcttc cccttttgcg gacgctttcc cggcttgcgc gcactgaaat tcctgtgcgc
     1321 atataagtaa catgttttaa gttaccttag ctgcagaaag ctcgggaaat gttcaccgac
     1381 ggcaacccgg ataaatgact gataaataat acacttgaga ttgccgcggg agtttgattc
     1441 cagcacgatg tgcctgactc taaggtcact tacagcggaa aatacgttag gtcccagttt
     1501 gagaaaaggt gggtagaacg tagaatggta ctcgatggct cggtttacca gcatagcacc
     1561 tttgattcat ttaaattgca gtatgaaagt gtatagtaac taacaacgaa gttacttact
     1621 tggggtgggc ccttgctctc cagatatgcc caacattgga tctcgattgc agttctgatc
     1681 gtctgcatca cagcaccgct gcgcggagat gaagtgaaaa gcgccgcata caggcttcca
     1741 ggcaagcgag ccatggccga gatggcctgg gacgggatgt gccgatcgga gccggacgag
     1801 cgagtctgga caggcatgaa ggcaccgtcc aacgcgagac aaacttgctc cgcgaaccca
     1861 gacgtgctgg tcgacagcgc ggattggacc gcaggccgac ctgaggcaag ccttgacacc
     1921 ctggaacccg gcggcgcgag tctcaaccaa atcagcagtg agcaggccgc gtcggtggtc
     1981 gccgccctgg cttccgtgac tccggagaaa cctcctgccg gcaccaaggt cccggaaccg
     2041 cccacgctcg cgctgctggg cgcgggaatg atcgcaacct cgagtctctc gcgcgtcacg
     2101 cggcaaccaa agtgcaagtt gcgggtggtc gcaacccgct tgggcgctgt tctccaacac
     2161 tgaaccgctc ctgttcggcc gtctccgctc aatcgctttg cctgcgaaac tggcaacatt
     2221 ttgaacagtc tcagcaactc aaggtgaaca cctccgaatg cgcgggcaag aacatgcggc
     2281 ggaggtagcg tgcttcatcg cacaccgaca aggcgttgag tgcgcggtag tcgaaagatt
     2341 tcgcccatac cacaatgtgg gatgtcgaag tttttctggt gtgcgcccct cgcctcactg
     2401 accaattgcc gcgccgtcgt gatgaacgcc tttattggcc gtcactgcct ttttctcgcc
     2461 agttttgtct ggttcgcgtc ccgtcactgg ctggcacggc gccataggtc tcacattgtg
     2521 ggacacaccg gaacgagcat tcgacatggt cgatcgcttt tacgtgatcg tacacggcgt
     2581 gcatcttggg gttcggtggc atctgttctg cggtccgcct tcgacgaatg cggagggctg
     2641 tgcggatggg gatgccgggt tttccacagc ggcaccaatc ttgtaacact cctgtaacaa
     2701 ttgcttcggt ggctgcgtct cttcaacgtg aagataggag aggagttggc gatgttttgc
     2761 gaaatctccg cgaaatctcc aaaatgcgcg cattccggac ttttacttac gtccgcggca
     2821 ggccataata gaggcagtac atagcgtcct ctattaacaa tcaggctcac agttcaggcc
     2881 cagaagtaag ctgtctgaca atgtgggagg agtgtaatgc aagtcgaatc tgatcatact
     2941 gctgagatta atcaacgcga gcgcatctgg tttttccccg acaagttctg gcaggccacc
     3001 aagggcatgc cgcaagatga agtctccaag ctgatggcag aagtggagca gtacgcggaa
     3061 gcgaagaacg tggaagcgct gcgcaagtat cccttcgtgt tcgtcggtga tccctacaaa
     3121 gaccataaca acgcagctta gggtgctgcc cgtagcggcc agcccgtctg tcgctatgcg
     3181 accaccgctt ctttattccg cgatcagacg cccggggcac cactacagcc gctcacagca
     3241 gaagccgtag ggcggctagg tgctggcctt ggcgatttct ccacggcgat gctgggtcgg
     3301 ctagatcgca gccggcgtgg ttggcttgcg gtgcgcgggg agcggccgga tcatgatggg
     3361 cattttgatg caggtttcca gcaagtaccg cgcccgcgcg agttgctcgc cgaacgggct
     3421 gatcgagctt gtcaaaccct gcgcgtggag gatgagcact cgattgccgc gctccacctt
     3481 caccgacaag atccgctgat cgtgaagttc gtcgagcgcg ttggccagtc gcagcacacc
     3541 cgcgagtggc atcagctcgc cgcggcgctg cgccggcaag ccagcgaaga tcgggtgatt
     3601 ggtaggtggc aggcctcccc gatgaaagtg cgccacgatg gcgacgcatt gcaggtactc
     3661 ctcgctccag cccactggcg gtttcaggtt ccggatcatg cgatacccgc gcttgcggtg
     3721 gcctccggct tttccgcggc caacgtcctg catcaccgcg gcggcctcaa gaatctggcg
     3781 tgacatttca gattctgcaa gaatgccttc gcgcgccaga ccgtcataaa tctccagcgc
     3841 aagggtggtg acgagttgtg catgcttgtc gtcgggatcg aggaacgcgg cccaggtgcg
     3901 catcttttcc agcgccgcct tggccagcgc ttctccagcg ggcaactcgg cacgccattt
     3961 ctgccagagc gattcccttc ccatcatctt tttgcggtaa agcgcgatgc gcttctgtcg
     4021 ctcgtcggat atggtctgct gccacagagc gcgttcctcc aggccgacct cggcatgggt
     4081 tttcaccatg gcccacagca cgtcgaagtc gtggacctcc ccgagggcgt cctgcaaatc
     4141 acgcaggtcg tgcgaccaga gcggttgccg ctccggcaga aaattctcga ccgtgtaacg
     4201 aaagcgtttg atgccgatcc gcaactggtg aaacgccacc gcgctgcgat tgcgtaatgc
     4261 cgtgcgatgc agttcgtaag catcatgcca gcgttccagc gcaagatact ggaagaccag
     4321 tccattcagc ggcacccggg tggcgcgatc ggcgaggtgc gtgttgagcg ccaaccagcg
     4381 cttgcggtcg aagctgctga ccgcttcctt cgcgaccgcc ttcagagctt cctccttctg
     4441 ctgcagagaa tcgaacagga tctgccgcag cggatcgtcc ggagcggaaa gcttgccgac
     4501 ccactccatc atgacctggg tatcgcgcag atcgccgagg ctggcgaaca gccccttgcc
     4561 gagcttcttc atctgacgcc acgcgggatc gggatcgacc gacagaaa
//
LOCUS       DASRKK010000106        14562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1081668, whole genome shotgun sequence.
ACCESSION   DASRKK010000106 DASRKK010000000
VERSION     DASRKK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081668"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFC15_02325"
     CDS             complement(<1..941)
                     /locus_tag="VFC15_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09027.1"
                     /translation="MFLKELIQQSWIALRRNRMRSVLTMLGISWGVVSLVLLLAFGEG
                     LGGGILKATGSLGNNVIVIWPGQTSMQAGGQRAGRKIHYEYEDIQALRAEVPIIRAVS
                     AESDTDFGFKAGTRVVSIQTRGVEFPYAAMRKLVAAEGRNIEEGEFASRKPVVLLGHA
                     AAKRIFQGAPAVGQTVLIQGRSFEVVGVMDTKIQDSMYEGPDNEQAFIPYEVFNELTN
                     AKDPQIIVFQPISAELHSQALVAARNVIGRRHHFEPRDNKATPEWDTVESGQMLKAFT
                     IGLDALLGIIGALTLGVGGVGVMNIMLVSVTERTREIG"
     gene            complement(955..2214)
                     /locus_tag="VFC15_02330"
     CDS             complement(955..2214)
                     /locus_tag="VFC15_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09028.1"
                     /translation="MNLLDIFKQTLATLRAHKMRSFLTMFGIIWGIASVILLVGLGLG
                     FSKDQKKRMETLGKDLVIVWGGRTSSQVGGLAAGRQIVLNIDDARLIKKACYFIKAVS
                     PELQRTVTETSQFNSAARQVSGIWPSYQDFRSLILSEGRLMTDDDEAEARRVVILGSN
                     AARQLFPGQPAVGATVNMARYPYTVIGVLAEKKQNSNYSGPDNDKIYVPYSAMARDYP
                     PPPAKGEIINKGYVSDIVMEVADPESHDAAVLQVRRVLGRMHHFEETDKDALFIWDTM
                     EFAKVLARIFAVITIFFGCVAVTTLALGGIGVMNIMLVAVTERTREIGILKAVGATKA
                     DILKQFFSESVALTILSGIIGYVVGVGICLLLNAAPLPDFVPKPIISAIAIVGSLLTL
                     SLITVSAGMYPAKRAADMDPIECLRYE"
     gene            2563..5892
                     /locus_tag="VFC15_02335"
     CDS             2563..5892
                     /locus_tag="VFC15_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM09029.1"
                     /translation="MFRTPLLKIALLISVACAAAAPAWAQWTTPTIDGQINSGEYGNN
                     NQLNNAGNTGQTWYMTWDATNLYVGIANANVNEGLVLYVADPNNNSTGNLTGFNYDGV
                     NFSSLPFRATFVTYVKSGYREYRNSNGGGGWGSQTANYGQYADDGGGTREVAIPWSAL
                     TGGGIPSQFVFFGYLVSAGGYVYGQAPSDNNIGGFAGLNATATQYFAVVNTGNGTSTP
                     PFSMEQPGGFSTQDKAGFYHNTFDPFYRDQEGAVPENTQVTLRFRTLHSSGIWSVNAR
                     AYLFDSASGNTTGPVDTDMPFDQNITINGTEYDVWKTTLTMPSSTSVYYYKFHIYRGP
                     STSPTNGWYSDDYLDDNDNVHKDGTGAASDGEPFPSFQFTVFDPNFQTPAWLQNANVY
                     HILPDRFRNGDQTNDYCRTGSTTGCPTFYGGGGTNTVIHYDIWNTQMCDPRNPNSPCY
                     NNFTQFYNGDLLGVQNELDYIQSLGFDTIYLNPIFMARSYHRYDTDQYLHIDPALGGD
                     AAFTALQAEMNRRGMKVILDGVFNHSSSDGLYFDRYHRYPTDGACESLSSVWRSWIHF
                     NDSHVPCNSSDYTGWFGFDSLPTFDHTNPAVQDFFYRGPGNVTQYWYNQGANGWRFDV
                     ADDGNFGHLWWNDYRTFAKSYNANGPLIGEIWPNASQWLAGDQMDSVMNYRFRKNITG
                     FVRNADWSDDNNNGTNNIPGLTPSLFDHAIRAVRDDYPPQATAAMLNLLDSHDVNRAL
                     FVMTETGDTGLVQAKQRLKLAALFQFTYIGAPMVYYGDEIAVNSPSQTSSANGPIGDP
                     YTRPPYPWLDQDGNPNIYGPPDTNMQAFYTKLAHIRKLYQVLRQGSFVTLLTGDTQQQ
                     NTAPSTYAFARTMQGQDAVVVALNNGPASNQASIPVAGIYTDGTSLYDVLGSGSYTVG
                     SGAVQVTLPARTGVLLVRGPIGNNSPSVGAGITLSPGLNGSGWSNSPVTVELRGNDSG
                     SGIEQLRYWIDDGPVNVAEGNSAALPIAAEGSYVVGLRAIDNAGYVSRQATQIVRIDL
                     QAPAVAVTGVQPGATYSGNAPAAGCTTIDALSGVATKASVSISGGNGRFTATCSGGKD
                     NAGNVAQPVSVTYFVVGSTNH"
     gene            6025..6417
                     /locus_tag="VFC15_02340"
     CDS             6025..6417
                     /locus_tag="VFC15_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09030.1"
                     /translation="MLKSIAVVLGSYLLSVVLVLATDPLLSRLFPGDFVKGHVPSDTA
                     LMASTACFVVVSILCAWLCARFAPGRAARHVLWFFVLGEVMGIAAAIPNWSKGFPHWY
                     WLSWLLTWPVSCWIGLLLAGRRPDSHSA"
     gene            complement(6452..7468)
                     /locus_tag="VFC15_02345"
     CDS             complement(6452..7468)
                     /locus_tag="VFC15_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZM09031.1"
                     /translation="MEVVAGVDLGGTAVNYTIVDQEGKFLIEGLCEHPALAKQGPDIC
                     LQQIADGLKITADKAGIAVSDIVAVGLDTPGPASASGVLSARGSTNFVHAKWAGFDIR
                     EGLAHKLGKPVLYLNDANAGALWGHYSIFGPNSKETSVSIVIGTGNGGGIIIDGNVVK
                     GKNGFGGELGHVLIPYQSIAGTPGMKPECNCGRTGDLESVCSLTAIERTLLPYFLPRY
                     PGHELGKVDLHTAAKLVRGMAEKGDPLSKDVFRVQAHGLGLFFDEMINTFDPDALIIG
                     GGALETTLEFQRWFIDEVRAGMPSQREEQVGIPIHVMPNGDTAGARGAAIEALKLARQ
                     GGLA"
     gene            7682..10021
                     /locus_tag="VFC15_02350"
     CDS             7682..10021
                     /locus_tag="VFC15_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM09032.1"
                     /translation="MIGQTISHYRIVGKLGGGGMGVVYEAEDTRLGRHVALKFIPDNL
                     LSDRKALDRFIWEARAASQLNHPGICTIHDIGEMDAHPFMVMEKLEGVSLKDRMLGKP
                     MEVDEILDICIQVADALAACHAQGIIHRDIKPANIFITRSGQVKILDFGVAKLAPGLR
                     AGAAALDTELSVAGDIFGTALYMSPEQARGEELDPRTDLFSLGVVLYQMATGQKPFAG
                     TNTITTLEAVMNRKPVSPLKLNPALPKDLEGILGRAMEKDRGNRYPNALAMKGDLQSL
                     RRETEPGMTETGRHRPPLPYRISSNTFESSSRRQTYILLGVIAVLITVLVPMGAWWFK
                     QRPGASGRNTVAVLPFENMNGDVSVDFLRFALADEVANALTYTRTLDVRPSSMTRKYT
                     GVEEDPRKVGNELHVSTVVTGHFLKQGDRLMVTLEAIESSTDRLLWQTTVTAPADDLI
                     NLQSAMSAQVNQGMLPALGIAGGALNAGSRPKDPAAYDLYLHSLALAHDPGPNKDAIV
                     VLEHAVQSDPGYAPAWEELGLRYYFDSSYSDGGEEAFQRSTKACERALTLDPNRIVAA
                     GQLITNQVERGELGKAYEQAQVLLQRRPDSAVAHFVMGYVYRYAGMLEQATKECNTAL
                     SLDPGNFTFRSCAWPFMEMGQHGARSGLHPPRRRVGMGGLCDAIAALTGRRNRKGSRG
                     RDPNADHSALPPRFIGGLPRTAASGGSRPHGAGSGNQRPGCARCRTSLLPGSHLRRLR
                     QEAGSAASAQRRGRAELLCLLQSAPGSDAEEGAARARFQ"
     gene            complement(10523..10927)
                     /locus_tag="VFC15_02355"
     CDS             complement(10523..10927)
                     /locus_tag="VFC15_02355"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HZM09033.1"
                     /translation="MRNLVAYVDGGSHGSPGPSGIGVVIEQTGSETVRIARSIGRQDN
                     NVAEYVALLEALQHAVSVNASTLHVFSDSEIVVKQMTGQYACRSPRLYSLNWTCRKLA
                     RSLDFSIAHISRELNLEANELANLAVRSRQTA"
     gene            complement(11848..13464)
                     /locus_tag="VFC15_02360"
     CDS             complement(11848..13464)
                     /locus_tag="VFC15_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM09034.1"
                     /translation="MDLGAGVRKLALGALAVSSFFAGSSTNAHAQAASAKKPNILVIF
                     GDDIGYWNVSAYNLGMMGYKTPNIDRLAHEGALFTDAYAQQSCTAGRAAFITGQSPIR
                     TGLLKVGLPGSKLGLQKEDPTIADLLKPLGYMTFQNGKNHLGDRNEFLPTVHGFDEFF
                     GNFYHLNAEEEPENVDYPRNPSFKSEFGPRGVFKCKATATDNPAPSDPRFGPWGKQTC
                     EDTGPLTKKRMETADDEFLAVTLEDMDRAVKAGKPFFIWHNTTRMHIWTHLPPQYAAM
                     ENQKGLYGAGMTQLDDNIGVILKKLDDLGIADNTIVIFTSDNGAEVMSWPDGGTTPFN
                     GEKNTSWEGGYRVPFVIRWPGTVKPGSVFNGTIAHEDWATTLLAAAGVPDVKEKLLTG
                     YQAGGTTYKVHLDGYNLMPYLKGDTDVDPRREFLYWTDDGELAALRYDKWKALFLEQP
                     AHGFSVWETPFVSLRVPKLFDLHADPFERAEMEGIDYSHWRLDRVFLLTPAQAFVGQW
                     LTSFKQFPPRQKPASFNLDQVMKQMAESSD"
     gene            complement(13729..>14562)
                     /locus_tag="VFC15_02365"
     CDS             complement(13729..>14562)
                     /locus_tag="VFC15_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09035.1"
                     /translation="ALLAKAKQLYDQGAIGELLSVELSLGRNDPTGAWEYPPPPGLAP
                     DNFDWDTWQGTVPKKAFDPITFARWRCWKEYGTGVAGDLMVHLVSGMMFVTGINQAPT
                     RAFASGGIVRWKDGRNMPDLQAAIFEYGSVPAYVRLTLGTETAEMTRLMGPKGMLEIR
                     ERDFTYTPQRGIDTAPSYYAQGFPAAMRTAYEKQWEDEHDPAPAHEFVAEAVTYSGPS
                     YDDLKPHLWNFFEAVRTRKPVVEDAVFGHHAAAACHMANQAYFRKATVTWDAASDTIR
                     G"
BASE COUNT         2951 a         4286 c         4289 g         3036 t
ORIGIN      
        1 ccgatttcgc gggtgcgttc ggtcacgctg acgagcatga tgttcatgac gccgacgcca
       61 cctacaccga gggtgagcgc gccgatgatt ccgagcaacg catcgagacc gatggtgaag
      121 gcctttagca tctggccact ctcgacggtg tcccattcgg gggtggcttt gttatcgcgc
      181 ggctcaaagt gatgacgccg cccaatcacg tttctggcgg caaccagggc ttggctgtgc
      241 aactccgcag aaatcggctg gaagacgatg atctgcggat ccttggcgtt ggtcaattcg
      301 ttaaatactt cgtaagggat gaacgcctgt tcgttgtccg gaccttcgta catcgaatcc
      361 tggattttgg tgtccatgac gccgacgact tcgaagctac gtccttgaat gaggacagtc
      421 tgacccactg cgggagcacc ttggaaaatc cgcttggctg cggcgtgccc tagcaaaacc
      481 acgggcttgc ggctggcgaa ctcgccttct tcgatgttgc ggccttccgc cgcaaccagt
      541 ttgcgcatcg ccgcgtacgg aaactcaacc ccacgcgtct ggatggaaac cacccgggtg
      601 ccggccttga atccaaaatc tgtatctgac tccgcactga cagcgcggat gatggggacc
      661 tcggcgcgaa gtgcctggat gtcttcgtac tcgtagtgaa tcttgcggcc agcgcgctgc
      721 cctcctgcct gcatgctggt ctggcccggc cagatgacga tgacgttgtt gccgagcgat
      781 ccggttgcct tgaggattcc gcccccgagc ccttcaccga acgcgagcag cagcacaagc
      841 gaaactactc cccaactgat gccgagcatg gtcagcacgg aacgcatgcg gtttctgcgc
      901 agtgcaatcc aactctgctg aatcaactcc ttgagaaaca cgctccacct cggttcattc
      961 gtatcgcaga cactcgatcg gatccatatc ggctgcccgc ttcgcgggat acatgccggc
     1021 gctcacggtg atgagagaca acgtcagcaa tgagcccacg atggcaatcg ctgagattat
     1081 gggcttcggc acaaaatcgg gcaggggggc agcgttcaac agcaggcaga ttccaacccc
     1141 tacaacgtaa ccaattatgc cgctgagtat cgtcaatgcc accgactcgc taaagaactg
     1201 cttgaggatg tcggctttgg tggcaccaac ggctttcagg atgccgatct cgcgggtgcg
     1261 ctcggtcact gccaccagca tgatgttcat gacgccgatg ccgccgagcg caagcgtagt
     1321 gaccgccacg catccaaaga aaatggtgat gacggcgaag atgcgggcca gcaccttggc
     1381 gaattccatg gtgtcccaga taaagagggc gtccttatcg gtttcctcga agtgatgcat
     1441 gcgccccagg acgcggcgga cctgcagtac cgcggcatcg tgcgattccg gatcggcgac
     1501 ttccatcacg atgtcggaga cataaccctt gttgatgatc tctcccttag cgggcggagg
     1561 gggataatcg cgggccatgg cagagtaagg gacgtaaatc ttgtcgttgt cgggaccgct
     1621 gtaattggag ttctgcttct tctccgccag tacgccgatc acggtgtagg gatagcgtgc
     1681 catgttgacc gtcgcaccta cggcgggctg gccggggaaa agctgccgag cggcgttgga
     1741 cccgagtatc acgacgcgtc gcgcctcggc ttcgtcgtcg tcggtcatca accggccttc
     1801 ggacaaaatc agagagcgaa aatcctgata ggacggccag ataccagaaa cctgccgggc
     1861 cgccgagttg aactggctgg tttcggttac cgtgcgctgc aactcggggc tgacagcctt
     1921 aatgaaatag cacgctttct tgatgagtcg ggcatcgtcg atgttgagga cgatctggcg
     1981 accagcggcg agtccaccga cttgcgagct cgtgcggcct ccccacacga tgaccaggtc
     2041 tttgccgagc gtctccatcc ttttcttctg gtctttgctg aagccgaggc ccaaccctac
     2101 cagcagaatg acggaagcaa tcccccagat gatcccgaac atggtcagaa aactgcgcat
     2161 tttgtgggcg cgcaacgtcg ctaacgtttg tttaaagatg tcgagtaagt tcataaatca
     2221 tgttccggca gcattcgggg gcgggcccaa aatactttga gccttcatga tttacatacg
     2281 ccaaaggtgg ggcgtaagtt ccgggcgtgt gccggcggaa tccggtaccg gccagccctc
     2341 gttggcctcc gggcgcggcg caaatgtgcg attcgggcgg gcaggggcaa aatttgctcc
     2401 gcgctgaggg gcaatttggt gtacatttta tgacgtttgc ctagctctat tagcgcaagt
     2461 tgatgtagga ctaaagttct atcagctttc tacttctgca acgcaagcat gacttctcaa
     2521 accgctggcg aaaccaaagc cagagaactc gcgaggattc ccatgtttcg tactcccctg
     2581 ctgaaaattg ctcttcttat ttcggtcgcg tgtgcggctg cggctccagc gtgggcgcag
     2641 tggacaacgc caaccatcga tggccaaatc aacagtggcg agtatggaaa caacaaccag
     2701 ctgaacaatg ccggcaatac cgggcagacc tggtacatga cgtgggacgc caccaacctc
     2761 tatgtcggca tcgccaacgc gaatgtcaat gaagggctgg tgctttacgt cgccgacccg
     2821 aacaacaact ccactggcaa ccttactggt ttcaattacg acggagtgaa cttttcatca
     2881 ctgccgttcc gggcgacgtt tgtgacgtat gtcaagagtg gataccggga gtatcgcaat
     2941 tccaacggtg gcggcggttg gggctcgcag accgccaatt atggtcagta tgccgacgat
     3001 ggcggcggca cgcgtgaagt cgcgatcccc tggagcgcac tcactggcgg tggaattcca
     3061 tcgcagttcg ttttcttcgg gtacctggtt tccgccggcg gatacgtcta tggccaggct
     3121 ccctccgaca acaatatcgg cggcttcgcc ggcctgaatg ccaccgctac gcagtacttc
     3181 gcagtggtga acaccggcaa tggcaccagc acgccgccat tctccatgga gcagccgggg
     3241 ggcttcagca cgcaggacaa ggcgggcttc tatcacaaca ctttcgatcc gttctatcgc
     3301 gaccaggaag gggctgtgcc ggagaacacg caggtcaccc tgcgcttccg cacgctgcac
     3361 tccagcggca tctggagcgt caatgctcgg gcctatttgt tcgactccgc ctcgggcaac
     3421 accacggggc cggttgatac cgacatgccg ttcgatcaga acattacgat caatgggacc
     3481 gagtacgacg tttggaagac aacgctcacg atgccgtcgt ccaccagcgt gtactactac
     3541 aaattccaca tttaccgggg tccgagcacc agcccgacca acggctggta cagcgacgac
     3601 tacctcgacg acaacgataa cgtccacaag gacggaaccg gcgcggccag cgacggcgaa
     3661 cccttcccgt cattccagtt cacggtcttt gatcccaatt tccagacgcc ggcgtggctg
     3721 cagaatgcca acgtgtacca catcctcccc gatcgcttcc gcaacggcga ccagaccaat
     3781 gactactgcc gtacgggatc gacgacgggc tgtcccacgt tttacggtgg cggcggtacg
     3841 aacaccgtaa ttcactacga catatggaac acgcagatgt gcgatccgcg caatccaaat
     3901 tcgccctgct acaacaactt cacccagttt tataacggcg atctgctggg tgtgcagaac
     3961 gagctcgact atattcagtc actcggattc gacaccatct acctgaaccc gattttcatg
     4021 gcgcgctcct accaccgcta tgacaccgac cagtatctgc atatcgatcc agcgttgggt
     4081 ggcgatgcgg cgtttaccgc gctgcaagcg gagatgaacc gccgcggaat gaaagtgatt
     4141 ctggatggcg tgttcaatca ctcgtcgtcg gacgggctct actttgaccg ctaccatcgc
     4201 tatccgacag acggcgcttg cgagtcgctc agctctgtgt ggcggagttg gatccacttc
     4261 aacgacagcc atgtcccgtg caattcgtcg gactacaccg gctggttcgg attcgacagc
     4321 ctgccgactt tcgatcacac taacccggcg gtacaagact tcttctatcg cgggccgggc
     4381 aacgtgacgc agtactggta caaccagggt gcgaacggat ggcgcttcga tgtggccgac
     4441 gatggcaact tcggtcatct gtggtggaac gattaccgca ccttcgcgaa gtcctacaac
     4501 gccaacggtc cgctgatcgg cgaaatctgg ccgaacgcga gtcaatggct ggcgggcgat
     4561 cagatggact cggtgatgaa ctaccgcttc cgcaagaaca tcacgggttt cgtgcggaac
     4621 gcggactgga gcgacgataa caataacggc acgaacaata ttcccgggct cactcccagt
     4681 ttgttcgacc atgcgattcg cgcggtgcgc gatgattatc cgccgcaggc caccgcggcc
     4741 atgctcaacc tgctggattc gcacgacgtg aatcgcgcgt tgttcgttat gaccgagact
     4801 ggcgacaccg gactggtcca ggccaagcag aggctgaagc tggctgcgct cttccagttc
     4861 acctatattg gcgcaccgat ggtgtactac ggcgatgaga tcgcggtgaa ctcgccttcg
     4921 cagaccagca gcgccaacgg gccaatcggc gatccctaca ctcgaccgcc gtatccgtgg
     4981 ctcgatcagg acggcaatcc gaatatttac ggtccgcctg acaccaacat gcaggcgttt
     5041 tacaccaagc tggcgcacat tcgaaagctt taccaggtac tgcgccaggg cagcttcgtc
     5101 acgctgctga ccggcgacac ccagcagcaa aacactgctc ccagcactta tgcatttgcc
     5161 cgtaccatgc agggtcaaga tgcggtggtg gtggcactga ataacggacc agccagcaac
     5221 caggcatcga ttcccgtggc ggggatttat acggatggaa cgtcactgta tgacgtgctg
     5281 ggcagcggca gttacaccgt cggttccgga gcggtgcaag tcacgctacc ggcgcggact
     5341 ggtgtgcttc tggtgcgggg tcccattggc aacaactcgc cgtctgtagg agcgggcatc
     5401 accctcagcc ctggactgaa cggcagcggt tggagcaatt ctcccgtgac cgtcgaattg
     5461 cgggggaacg atagcggcag cggaatcgag cagcttcgct actggatcga cgatggccca
     5521 gtcaatgtcg ctgaaggtaa ttcggcggcg ctgccgattg cagccgaagg ctcctacgtt
     5581 gtgggactac gagcgattga caacgctgga tacgtcagcc gtcaggcaac gcagatcgtg
     5641 aggatcgatc tgcaagctcc ggcggttgcg gtgacgggcg tacagccggg cgctacgtat
     5701 tcgggtaacg ctccagctgc aggatgcact accatcgacg cgctctccgg cgtcgctacc
     5761 aaagcgtcgg tgtcgatcag cggaggcaat ggtcgattca cggcgacgtg ttccggaggc
     5821 aaggacaatg ccggcaacgt tgctcaaccg gtaagcgtca cctatttcgt cgtcggttcg
     5881 acgaaccact aactctaact gtcactctca tgggaatgcc gccctgcggg gcggcatttt
     5941 ttttgatatt gcatgacttg cgccgccgct ttaacgttgt gctcgagtcc agtcctggtt
     6001 tttcacttcg tggaggcgta accgatgctc aaatccatcg ccgtggtcct cggcagctac
     6061 ctgctctcgg ttgttcttgt cctggcaacg gacccgttgc tctcgcgcct gtttccgggc
     6121 gatttcgtca aaggccatgt tccctccgac accgctctca tggcgagtac ggcgtgcttc
     6181 gtggtcgtct cgatcctatg tgcgtggctc tgcgcccgtt ttgcaccggg ccgcgccgcg
     6241 cggcatgtgt tgtggttctt tgtcctcggg gaagtgatgg gcatcgccgc ggctattcct
     6301 aactggagca agggctttcc gcactggtac tggttgtcat ggctgctcac ctggccggtg
     6361 agctgctgga tcggcctgct gctggccggg cggcggcctg attcgcactc cgcctgagtt
     6421 ctcactcaga acagacgatc catgctggaa gttacgcgag accgccctgg cgcgcgagtt
     6481 tgagggcttc gatggcggct ccacgcgcgc ctgcggtatc gccattgggc atcacgtgga
     6541 tcgggatgcc aacctgctcc tcgcgctgcg agggcattcc ggcgcggact tcgtcgataa
     6601 accagcgctg aaattccaga gtcgtctcaa gcgcaccgcc gcctatgatc agcgcgtcgg
     6661 gatcgaaggt gttgatcatc tcgtcgaaga acagacccaa gccgtgggcc tgcacgcgga
     6721 agacgtcctt ggacaacgga tcaccttttt ccgccatacc gcgcaccagc ttggctgcgg
     6781 tgtggagatc gaccttgccc agttcgtggc cgggatagcg cggcaggaaa tacggcagga
     6841 gcgtgcgttc gattgcggtc aacgaacaca ctgattcgag atcgccggtg cgaccgcaat
     6901 tacattccgg cttcatcccc ggagtaccgg cgatgctctg gtagggaatc agcacgtggc
     6961 ccaattcccc accgaatccg ttcttgcctt tcaccacatt gccatcgatg atgatgccgc
     7021 cgccgttgcc cgtcccaatg acaatcgaaa ccgacgtttc tttactgttg ggtccgaaga
     7081 ttgagtagtg tccccacagc gcaccagcat tggcgtcgtt cagatacaac accggcttgc
     7141 cgagtttgtg tgccagcccc tcgcggatat cgaatcctgc ccacttggcg tgcacaaagt
     7201 tggtggagcc gcgcgcactg agcacacccg aggcactcgc tggaccgggc gtgtcgaggc
     7261 cgacagcaac gatgtcagaa accgcgatgc cagctttgtc ggcggtgatc ttgagaccgt
     7321 cggcgatctg ttgcaggcag atatccggtc cctgcttagc cagcgcagga tgttcgcaca
     7381 atccttcgat gaggaacttc ccctcctggt ccacaatcgt atagttgacg gcggttccgc
     7441 ccagatccac tccggccacg acttccacgg gattgtcctc ttcagtatgg caatgacgaa
     7501 cgcaccagat tagcagatga gggacgcatt ttggcggtgg gttgcggctg ggtagtcgat
     7561 ccgctagcgg gttgcaaacc actcgaccgg aaggtgcgct gtgaagagag cgagcattcc
     7621 cggttgaccg ctgctggcga gcagcctaac attaccgcgc atggacgtcg cggcgcctgg
     7681 gatgattggc cagactatct ctcactaccg catcgtggga aaactaggcg gtggtggcat
     7741 gggagtagtg tacgaagccg aggacacgcg cctgggccgc cacgtcgctc ttaagttcat
     7801 tcccgataac ctgctcagtg atcgcaaggc gctggatcgc ttcatctggg aggcgcgcgc
     7861 tgcctcgcag ttgaaccacc ccggcatttg cactatccac gacattggcg agatggacgc
     7921 gcatcccttc atggtcatgg agaagctgga aggggtgagc ctgaaggacc ggatgctggg
     7981 caagccgatg gaggtggacg aaatcctcga catctgcatt caagtggcgg atgcgctcgc
     8041 cgcctgccat gcccagggga tcatccatcg cgatatcaag ccagccaaca tttttattac
     8101 ccggagcggt caagtcaaga tcctcgactt cggtgtggcc aagctggcgc ccgggttacg
     8161 tgcgggcgca gctgcgttgg acactgagct ttcagtcgcg ggcgacattt ttggcacggc
     8221 gttgtacatg tctccggagc aggcccgcgg cgaagaactc gatccccgca ccgacctgtt
     8281 ctctctggga gttgtcctgt accaaatggc taccggacag aagccttttg ccggcaccaa
     8341 cacgatcact acactggaag cggtgatgaa ccgcaagccg gtgtctccgc tcaagctcaa
     8401 tcccgcgctc cccaaagatc tggaaggcat tctcggccgg gcgatggaga aagaccgcgg
     8461 taaccgctat ccgaacgcgt tggcgatgaa aggcgatctg cagtcgctca ggcgggaaac
     8521 cgaacccggc atgaccgaaa ccgggcggca ccgtccgcct ttgccgtacc gcatttccag
     8581 caataccttt gaatcctcca gccggcgcca gacctacatc ctgttgggcg tgatagcagt
     8641 gctcatcacg gtgctggtac ctatgggtgc ctggtggttc aagcagcgtc ccggagcgag
     8701 tgggagaaac acggttgcgg tgctgccgtt cgagaatatg aatggcgatg tcagcgtgga
     8761 tttcctgcgt ttcgccctgg ccgacgaagt ggccaacgcg ctgacctaca cccggactct
     8821 ggatgttcgc ccttcgtcga tgacgcggaa gtacacgggt gtcgaagagg accctcgcaa
     8881 ggtgggcaat gagctgcatg tgtcgacggt cgtaacggga cacttcctta agcaaggcga
     8941 ccgattgatg gtgaccctgg aggccatcga gagcagcacc gatcggctgc tgtggcagac
     9001 cacggttacg gctccggccg acgacctcat caatttgcag tccgccatgt ccgcgcaggt
     9061 aaaccaggga atgctcccgg cacttggcat cgccggcggt gcgctcaatg ccggcagccg
     9121 ccccaaagat ccagcggcct acgatctgta tctgcatagt ctcgccttgg cgcatgaccc
     9181 gggcccgaac aaggacgcca tcgtggtgct ggagcacgcg gtgcaaagcg acccgggcta
     9241 cgcgccggcg tgggaagaac tgggcctgcg ctactatttc gactccagct attccgacgg
     9301 tggagaagag gcattccagc gctcgacgaa agcctgtgaa cgcgccctga cgctggatcc
     9361 caatcgcatc gtggccgccg gacaactcat caccaaccag gtcgagcggg gcgagttggg
     9421 caaagcctac gagcaggcac aggtcctgct ccaacgccgt ccggatagtg cagtggccca
     9481 ctttgtcatg ggctacgtct atcgctacgc cggcatgctc gaacaagcta ccaaggaatg
     9541 caacaccgca ctctcacttg atcccggcaa tttcaccttc cgctcctgcg cctggccctt
     9601 tatggagatg gggcaacacg gcgcgcgcag cggacttcat ccacctcgac gcagggtcgg
     9661 aatgggcggc ttatgcgacg ccatcgctgc tcttacggga aggcgaaatc gaaaaggctc
     9721 gcgaggccgt gacccaaatg ccgaccactc cgcgctacca ccgcgattta ttggaggctt
     9781 gcctcggact gcggcctccg gaggaagccg accgcatggc gcaggaagcg gaaaccaacg
     9841 ccccggctgc gcccgatgcc gaacttcttt actaccaggg agccatcttc gccgactgcg
     9901 gcaagaggca ggcagcgctg cgtctgctca acgccgcggt cgagcagaat tactgtgcct
     9961 actccaatct gctcctggat ccgatgctga ggaaggtgcg gctcgcgccc ggtttcaatg
    10021 acctgctgaa ctcagccaaa gagtgtcggg atgcggccgt gtcctcgaag aacgagccgc
    10081 cgaccccgtg acctgcatac gtcggcggtt ttgcgaaatg acgcggaaca gcagctagac
    10141 ctcacaactc cggcaccaat ggaatgtgcc gctatgccga attgttcacg attggctttc
    10201 gaatcccagt ggcacggact cctcggattg aaagtcctca atgtctcgcc ggctgtagct
    10261 gtgaacggtg ccgcaagttt tacagcgcat ctcgaatgtg gcagggaagt ttagctgaac
    10321 ggtcggctca ttgcctgctt ccggaacaaa ctcgacgtcc ttaaatatct ggcgcttctt
    10381 acatccggga gtcttgcagt ccgcacacca gtagagatgc attggatcct cgtcaccgtg
    10441 agttttgacc gctgcataag cagtctaacc cgaagcgacg cgaagcaaaa taggtttggt
    10501 tcttaccttc gaggaaccct gatcaggccg tctggcggga gcggacggcg aggttggcga
    10561 gttcgttggc ttccagatta agttcgcggc tgatgtgggc gatcgagaag tcgagggagc
    10621 gggcgagttt gcggcacgtc cagttcaggg aatacaggcg tgggctccga caggcgtact
    10681 ggccggtcat ctgcttcacc acaatctccg agtcagagaa cacgtggagc gtgctggcgt
    10741 tcacggacac ggcgtgttgc agcgcttcca gcagggcaac gtactcagcc acgttgttgt
    10801 cctggcggcc gatcgaccgg gcgatccgca ccgtttcact gcccgtctgc tcgatgacca
    10861 ccccaatgcc agacggcccc ggactaccgt gggacccgcc gtcaacatac gctacaagat
    10921 tccgcatcga actcctggct ctgacaatgc cgcactatgc ggctggcgtc aagagccttg
    10981 cgtggttcgc tgacatatag ttttgcacac ggacctctgt gaaaaacgtg tgaacaaacc
    11041 acaagatgtt tcccagcgaa aattcaagtg ctgaaagttc aataggttgc acggcgaaat
    11101 tttgttcgca aaataactgg cgattgcacc aaccttgggc attgacatct cacttttccg
    11161 tattaggatg gctctcgtcg acggcagaaa agggttggca tcttaaacgt agctgacctg
    11221 aagtacgctg agtggtcagt cgagcggggc gacccgcaaa gactgccgga cccgaaaaaa
    11281 ccaaccgcac tgcgaggtgt cggctggggt ctggaacccg cgaaagcggt gaccgcgtca
    11341 agggctgtga cggtacatgc gtccacgccg ggggctccct cgcaggagtt gtcggctaga
    11401 gcataaccaa agtcacagcc ttttctattg cccaaaacct tgggcgcttc cccccttgaa
    11461 gttcaggtag ctggcaatca aattcgaccg tctgtgtcga gttagacatt cgactatttg
    11521 tttcctttat caacagaatt atgcaaaagc ctgtgcaaga ttcggatttt gcgggtttgt
    11581 agagtgtctt tctaaggact tgcacgtatt gcactcgcca cggtgcgatt gcgaaacctc
    11641 cgagcccatt cagagtctgt gaaaacaaag gagacgagtg tgcggtttgg aggagccaac
    11701 cgcagatccg ttcgactgcc cacgcgctgt tgggcgcctc ctccgctcag gatgagatcg
    11761 aagagtcagc gcaggatcac gcgacaaagg gcggcccgta agggaccgcc cgattgtggt
    11821 ggggctagtt gccccgcttg ccgccagtta gtcagaagat tctgccatct gcttcatcac
    11881 ctgatcgagg ttgaagctgg ccggcttctg gcgcggcggg aattgcttga agctggtcag
    11941 ccactgcccg acaaaggctt gtgcaggcgt cagcagaaag acgcgatcga gccgccagtg
    12001 cgaataatcg atgccttcca tctcggccct ttcgaaagga tcggcgtgga ggtcgaagag
    12061 cttgggaacg cgcagggaaa cgaagggcgt ttcccacacc gagaacccgt gagcgggctg
    12121 ctccaggaac aaggccttcc acttgtcgta acgcaaggca gcgagctcgc cgtcgtcggt
    12181 ccagtagagg aactcgcgac gaggatcgac gtcggtgtcg cccttgagat aaggcatcag
    12241 gttgtagcca tcgaggtgga ccttgtaggt ggtgccgccc gcctggtagc cggtcaacag
    12301 cttctctttg acgtcaggca cgccggcggc ggccaacagg gtcgtagccc agtcctcatg
    12361 cgcaatggtg ccgttgaaca ccgatcccgg cttgacggtt cccggccagc gaatgacgaa
    12421 gggcacgcga tatccgcctt cccaactggt gttcttctcg ccgttgaaag gcgtggttcc
    12481 accatcgggc cagctcatca cttccgcccc attgtcgcta gtgaagatga caatggtgtt
    12541 gtcggcaatg ccgagatcgt caagcttctt caggatgacg ccgatgttgt cgtcgagttg
    12601 ggtcatgccg gcaccgtaga ggcccttctg gttttccatg gccgcgtact gcggaggaag
    12661 gtgcgtccag atgtgcatgc gggtggtgtt atgccagatg aagaatggct tgcccgcctt
    12721 gacggcacgg tccatgtctt cgagcgtgac ggccaggaac tcgtcgtcgg ccgtctccat
    12781 gcgtttcttg gtcagagggc cggtgtcttc gcaggtctgc ttgccccaag ggccaaagcg
    12841 cggatcggat ggagcgggat tgtcggtggc cgtggctttg cacttgaaca cgccacgcgg
    12901 accaaactct gatttgaagc tggggttcct cgggtagtcc acgttttccg gctcctcttc
    12961 ggcgttcaga tggtagaagt tgccgaagaa ctcatcgaag ccatgcactg taggcaggaa
    13021 ctcgttgcga tcgcccaggt ggttcttgcc attctggaag gtcatgtaac cgagcggctt
    13081 gagcaggtcc gcaatcgttg ggtcttcttt ctggagtccg agcttcgagc ccggcaggcc
    13141 gactttgagc aagccggtgc ggatcgggct ctgtccggtg atgaaagccg cgcggccagc
    13201 cgtgcaactc tgctgcgcgt aagcatcggt gaacagcgcg ccttcgtggg ccaggcggtc
    13261 gatgttaggc gtcttgtagc ccatcatgcc caagttataa gcgctgacgt tccaataacc
    13321 gatgtcgtca ccaaagatga cgaggatgtt tggcttcttt gcgctggctg cttgcgcatg
    13381 tgcgtttgta ctgctgcccg cgaaaaagct cgagacggca agcgctccca gcgccagttt
    13441 tcgaactcct gctcctaagt ccatgtgtgt cctcttttca actacgacta gttagctccc
    13501 gggtggggag acgtagattt cttttggtca attcaagacg attcgcggag gtggagagaa
    13561 ttttccgttg tgacagggac tcccgcacca tgctgctttg cccgcgggag gtcgggagat
    13621 tcgcagatat tgcgcagcct tcagcactca cgagcacttc gttcattctg tgaccgatgt
    13681 gctgatgttg cgtgtgcagt ctactatatg caactcggcc agtttgcgct agccccggat
    13741 ggtgtcgctg gccgcatccc aggtgaccgt tgcttttcgg aaatatgcct ggttggccat
    13801 atggcacgcg gctgcggcgt ggtgtccgaa gacggcatcc tctaccaccg gcttacgcgt
    13861 ccgcaccgct tcgaagaagt tccacaagtg cggtttcagg tcgtcatacg aaggaccgct
    13921 atacgtaacc gcctccgcca caaattcatg agcgggagcg ggatcatgtt cgtcttccca
    13981 ttgcttctca taggcagtgc gcatcgcagc cgggaaaccc tgggcgtaat aactgggagc
    14041 cgtgtcgata ccgcgttgcg gcgtgtacgt gaaatcgcgc tcgcgaatct ccagcatccc
    14101 tttcggaccc atgaggcggg tcatctcagc ggtttcggtg cccagggtca gtcgaacgta
    14161 agcaggcacg ctgccgtatt cgaagatagc ggcttgcaga tcgggcatgt tgcggccatc
    14221 tttccagcgc acgatgcctc cgctggcgaa ggcgcgcgtc ggcgcctggt tgatgcctgt
    14281 cacaaacatc atcccactca ccaggtgcac catgagatct ccggccacgc ctgtgccgta
    14341 ctccttccag catcgccagc gcgcaaaggt aatgggatcg aatgctttct tgggaacagt
    14401 gccctgccag gtgtcccaat cgaagttgtc aggcgcgagg cccggaggcg gaggatactc
    14461 ccacgcgcca gttggatcgt tacgtcccag cgagagttca acagacagca attccccgat
    14521 agctccctga tcgtaaagct gtttggcctt cgccagcagt gc
//
LOCUS       DASRKK010000107         7250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1179606, whole genome shotgun sequence.
ACCESSION   DASRKK010000107 DASRKK010000000
VERSION     DASRKK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179606"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFC15_02370"
     CDS             complement(<1..486)
                     /locus_tag="VFC15_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZM09036.1"
                     /translation="MNPILASIVLGLTAAAANVFGGTVIVQRTWERRYLRYFVALGAG
                     FMLATAMVEMVPESLALAGRKGALLILIGYLIVHLFEHTITPHFHYGEETHADEFVHS
                     HKRMSVLVGLMIHTFFDGIAITSGFLISNWLGWVIFLAVFLHKIPEGFTVTSVMLASG
                     LS"
     gene            complement(575..1657)
                     /gene="ychF"
                     /locus_tag="VFC15_02375"
     CDS             complement(575..1657)
                     /gene="ychF"
                     /locus_tag="VFC15_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZM09037.1"
                     /translation="MKTGIIGLPQVGKTSLFVMLTKLSVANRSGSPREAHLGVTKVPD
                     DRLDRLAALYDPKKLIHASVEYTDVAALSQEALKETAFAANLRNVDALAHVIRAFDDP
                     SIPHLGAIDPLRDIKNIDLDLIINDLGQIEKRLERVEKDLKKMRTTELEREFELLKRA
                     KEHVETERPLREMEMTPEDKKRIRGFMFLSEKPILYVLNIGESTTLSKDSEEAVAKYG
                     IAEVAARPNTGALAVCAKVEAELAEMSDEDAAEFLSSYGLQESGLVRLIRKTYELLGL
                     ISFFTVGEDECRAWTVERGSKAVEAAGAIHTDLSKHFIRAETIHWDQLLEAGSEAEAR
                     SRGTLRLEGKEYLVKDGDVMHIRHSG"
     gene            complement(1862..2344)
                     /locus_tag="VFC15_02380"
     CDS             complement(1862..2344)
                     /locus_tag="VFC15_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HZM09038.1"
                     /translation="MSLVRFLMLLSLVMWVGGIVFFAFVLAPTVFHPGILPSRQLAGA
                     VVSRSLGILHWMGIVCGVVFLVTSVVDSQVVTGVPALFSARNLLVCAMILLTLISMFA
                     IATRMLDLRNQMVFIDNVPHDDARRVEFNRLHVWSTRLESTVLLLGLAVIFLTSRRLS
                     "
     gene            complement(2369..2572)
                     /locus_tag="VFC15_02385"
     CDS             complement(2369..2572)
                     /locus_tag="VFC15_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09039.1"
                     /translation="MKNVLSFHRIRGNWPIVKLIALNYAVCQTFVIAPTPPSWYFDMR
                     IVGFRPYEAGSSEPNPPSASTSI"
     gene            2578..4563
                     /locus_tag="VFC15_02390"
     CDS             2578..4563
                     /locus_tag="VFC15_02390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM09040.1"
                     /translation="MSLAVHKWSSVVLLVWLWFAAGAQPLHSLEPSTLTLTPSTVEVS
                     LSTDAELICDPRGDMSLAAAQAGQYVPLGSELTTSDQCHGYWVRFNLRAPSVSPGGWV
                     LQLSRGWAHADLYYEQDGRISVLRTGTFLPPSERALASSFLLLPLPLESNRATTFYLH
                     LAGDTGRYGEARTLAGTIQRLDANQVTRRNALFAQGIYGGIILALVLYNLILYGAIAE
                     RAYLYYSLYVLTFGSVWILRTGFFFQYLWPHHPVVESEAPFYFVVLAILFSSLFVRQF
                     LATRDQSKWVDRTLLAILFFSAALCVLRISGDRNTSTLLLAIDGLVTTIFFAAVGVLF
                     LLRGFRPARFFLAAWALQLIGNVLYIVAFVRLIPFNFVTYNAAQIGSGVESILLAFAL
                     ADRVNLLKREKDKKQLQYTRELQEQVTQRTEELTSTVEKLKTAAITDPLTGLSNRRHV
                     DAAIQPWIAELQRARIRNSPEAPRRYLAVCLADLDHFKLINDELGHAVGDKVLQAAAD
                     TLRQNVRATAILARWGGEEFLILDHVTGPQEDLLMAERLRLSIVDECQPIFLAIGRSV
                     SLSLGVLRYPFSVTYPELLSWDHCLALADHALYRAKKAGRNCWQCYRPNEAALRVAIQ
                     SHGIEEVRRLLRMHTEQAFEMGLIEVVEQVTSDVRVL"
     gene            complement(4620..5747)
                     /gene="metX"
                     /locus_tag="VFC15_02395"
     CDS             complement(4620..5747)
                     /gene="metX"
                     /locus_tag="VFC15_02395"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZM09041.1"
                     /translation="MSEALEPTREGDLTFAYGAPFRLEAGGSLQPVSVRYAVYGELNQ
                     RRDNAVLICHALSGSARAADWWPEMFGSVFDLYRNCVICTNILGSCYGSSGPTSINPA
                     TGKPFGPDFPIVTIRDIVRAQAHVLDELGVKRVRIVIGASIGGMQALQWTVDYPERVE
                     HCIAIGAAPLSAMGLALNHLQRKAIELDPDFKAGRYAAGEQPAGGLGLARGIGMCSYK
                     SADLFDERYARKPNRTGEDPLRSFADRYDVAGYLDHQGEKFVTRFDANAYLVISKAMD
                     TFELGRGYASEAEALQRIKAHVSLVGISSDWLFPAKDVRALADRLEAAGAQCDYTEFE
                     TSHGHDGFLAEMDAIATVVNGSFHARRLAVVHKNAVRKLGG"
     gene            complement(5750..7072)
                     /locus_tag="VFC15_02400"
     CDS             complement(5750..7072)
                     /locus_tag="VFC15_02400"
                     /inference="COORDINATES: protein motif:HMM:NF006096.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HZM09042.1"
                     /translation="MSDKPEFHFDTLAIHGGQDPDPTTKSRAVPIYQTTSYVFDDADH
                     AARLFALQEFGNIYTRIMNPTTDVFEKRIAALEGGLAGLALASGQAAETLIITTLASA
                     GDEIVSTTSLYGGTYNLFHYTLPKLGITVKFVDADDFDGLRKAIGPKTKALYTETLGN
                     PKLDIADVEELAKIAHENGIPLVIDNTCASPALCRPIEWGADIVVNSATKYIGGHGTS
                     IGGVIVDGGKFDWAASGRFKDFVEPDPSYHGVSYTGAFGPLAFILKARVQGLRDTGAS
                     LSPFNAFLLLQGTETLHLRMERHSENALKVAEYMSSHPDVEWVNYPGLPTSKYYQRAK
                     KYLPNGTSSLVTFGIKGGYEAGKRFINATQLFSLLANIGDAKSLVIHPASTTHQQLSE
                     SEQRETGVTPELVRLSVGIEDVRDIIADIDGALQVATGKKVATQVAGD"
BASE COUNT         1430 a         2142 c         2148 g         1530 t
ORIGIN      
        1 gctgagtccg ctggccagca tgaccgaggt cacggtgaag ccttcgggta tcttgtgcag
       61 gaaaacggcg aggaagatga cccatcccag ccaattcgaa atcaggaagc ccgaggtgat
      121 ggcgatgccg tcgaaaaacg tgtggatcat cagtcccacc agcacggaca tgcgcttgtg
      181 ggaatggacg aactcgtcgg cgtgggtctc ttcgccgtaa tgaaaatgcg gcgtaatggt
      241 gtgctcgaac aggtggacga tgagatagcc gatcaggatc agcaatgcgc ccttgcgccc
      301 cgcaagtgcc aggctctcgg ggaccatctc gaccatggcg gtggccagca tgaagcctgc
      361 gcccagcgcc acgaaatagc gcaggtaccg gcgttcccat gtcctctgca cgatgaccgt
      421 gccgccgaaa acattggcgg cggcggcggt cagtccaagc acgatgctgg ctaggatggg
      481 attcacgggc gcgagaagga tagcagaaga gtggtcggcg gccggtggcc agtggctagc
      541 aagaaaatct atgccgagaa ctccgaactg accactaacc actatgccgg atgtgcatca
      601 cgtcgccatc cttgacgagg tactccttgc cttcgaggcg cagcgtgcca cgtgagcgcg
      661 cttccgcctc cgacccggct tcgagcaact ggtcccagtg aatggtctcg gcgcgaatga
      721 aatgcttcga caggtccgtg tgaatcgcgc ctgcagcttc caccgctttg gagccgcgct
      781 ccaccgtcca ggcgcggcat tcgtcttccc caacggtaaa gaatgaaatc agtcccagta
      841 actcgtaggt cttgcggatg aggcgcacca gcccgctttc ctgcaagcca tagctggaaa
      901 gaaattcagc cgcgtcctcg tcgctcatct cggcaagctc ggcttcgacc ttggcacaca
      961 cagccagtgc tccggtattg ggacgcgcgg cgacttcggc gattccgtat ttcgctactg
     1021 cctcttccga gtccttgctc agggttgtgc tctcgccaat gttgagcacg tagagaatgg
     1081 gcttctcgct caggaacatg aatccgcgaa tgcgcttctt gtcttcgggc gtcatctcca
     1141 tctcgcgcag gggtcgttcg gtttccacgt gctccttggc gcgcttgaga agttcgaatt
     1201 ccctttcgag ttccgtcgtt ctcatcttct tgaggtcttt ttcaacgcgc tccaagcgct
     1261 tctcgatctg gccgagatcg ttgatgatga gatcgaggtc gatgttcttg atatcgcgaa
     1321 gcggatcgat ggcgccgagg tgcggaatcg agggatcatc gaacgcgcgg atcacatggg
     1381 ccagcgcatc gacgttgcgc aggttagcgg cgaaggcggt ttccttgagc gcctcctggc
     1441 tgagggccgc gacgtcggta tattccacgc tggcatgaat cagtttcttg gggtcataga
     1501 gcgcggccag tcggtcgagc cggtcatcag ggactttggt gacgccgaga tgggcctcgc
     1561 gcggactgcc tgaacgattt gcgaccgaca gcttggtcaa cattacgaac agcgaggtct
     1621 tgcctacctg cggcaggccg atgattccag ttttcatgtg atgatggcct ctatttccca
     1681 accgtcagcc gcgcatgaac cgggccgagg aatctatgaa tgtaagcgat cgcggctgcg
     1741 gtcgtgctcg aagtgcgcca tccgcaactg tcgcccgtcg attttattag gatgcaaggc
     1801 cccatcgcct cgcacggttc tcgacacgca cggaaagaaa ctttcggctg gggccgaccc
     1861 tctatgataa acgccgtgag gttagaaaga taaccgccag tccaagcagg agcaccgtac
     1921 tttccagtcg ggttgaccac acgtgcagtc ggttgaactc gacacgccgg gcgtcgtcgt
     1981 gtggcacgtt atcgatgaag accatctggt tgcgcaaatc caacatgcgc gtcgcgatgg
     2041 cgaacatgct gatcagcgtc agcagaatca tggcgcagac caacaggttt ctcgcgctga
     2101 acagtgcggg cactccggtg acgacctgcg agtcaacgac cgacgtgacc aggaagacca
     2161 cgccgcagac aatccccatc cagtgcagga tgccgagact gcggctcacc accgcacccg
     2221 ctagctgacg actggggagg attccggggt gaaagacggt gggggcgagc acgaaggcaa
     2281 agaagacgat gccgccaacc cacatcacca gcgaaagcag catgagaaat cgaacaaggg
     2341 acatgaggac ctcaggagaa aggctattct aaatgctggt ggaggcggag ggcggattgg
     2401 gctcggacga cccggcttcg taggggcgaa atcccactat tctcatgtcg aaataccacg
     2461 acggtggggt gggcgcgatt acgaaagtct gacacactgc atagttcagg gctatcagct
     2521 tgacaatggg ccagttcccg cgaatcctgt ggaaggagag tacgttcttc acttcgtatg
     2581 tctcttgcgg tacataaatg gagtagcgtc gtcctgcttg tctggctttg gtttgcagcc
     2641 ggcgcgcaac ctctccacag ccttgaaccg tctacgctaa cgcttactcc ttcgacagtt
     2701 gaagtttcac tctcgactga cgcagagttg atttgcgatc cgcggggaga catgtccctg
     2761 gcggcggccc aggctgggca atatgttccg ctgggcagtg aactcacaac ttcggaccag
     2821 tgtcatggct attgggtgcg cttcaatctg cgtgccccca gtgtttcgcc cggcggatgg
     2881 gtgctacaac tgtcgcgggg atgggcgcac gccgatctct actacgagca ggacggccga
     2941 atctccgttc tgcgaactgg gacgttcctg ccaccgtcgg agcgagcgct ggcttcgagt
     3001 tttctgctgc tgcccctgcc tctggaatcg aatcgtgcga ccacctttta cttgcacctg
     3061 gctggcgaca ccggtcgata tggcgaggca cgcacgttgg ctggaaccat ccagcggctg
     3121 gacgccaacc aggtcactcg ccggaacgca ttgttcgcgc aaggcatata tggcgggatc
     3181 atccttgcgc tggtgcttta caacctaatc ctgtacggcg caatcgccga gcgtgcttat
     3241 ctttactact cgctctacgt actgactttt ggctcagtct ggattttgcg caccggtttc
     3301 ttcttccagt atttatggcc gcaccatcct gtggttgagt cggaggcgcc attttacttc
     3361 gtagtgttgg ccatcctttt cagcagcctc tttgtgcgtc agttcctggc cactcgcgac
     3421 cagtcgaagt gggtggaccg caccctcctg gcgatcctgt tcttctccgc agcgctgtgc
     3481 gtactccgga tttccggcga ccgcaatact tcgaccttgc tgctggcgat tgacggcctg
     3541 gtgaccacca tcttcttcgc tgcggtgggc gtgctattcc tgctgcgcgg ctttcgtccg
     3601 gcgcgcttct ttctcgcagc gtgggcgttg caattgattg gaaacgtgct ttacatcgtt
     3661 gccttcgtgc gattaatccc atttaacttc gtcacctata atgccgccca gattggatca
     3721 ggagtggaga gtatcctgct cgcgtttgcc cttgccgacc gggtgaacct gctcaagcgg
     3781 gagaaagaca agaaacagtt gcagtacacc cgcgaactcc aggaacaggt gacgcagcgc
     3841 actgaggagt tgaccagcac ggtcgagaag ctgaaaacgg cggcgatcac cgaccctctg
     3901 accgggttga gcaaccgccg ccatgtggac gccgcgatcc agccctggat cgctgaactg
     3961 cagcgggcgc ggatacgaaa ctcacccgag gcaccacgtc gctatcttgc ggtctgcctt
     4021 gccgacctcg atcacttcaa gctgattaac gatgagttgg gacatgccgt gggcgacaag
     4081 gtgttacagg ccgcggccga cactctccgg cagaacgtgc gcgctaccgc catcctggct
     4141 cgctggggtg gggaggagtt tctgatcctt gaccacgtga ccgggcccca ggaagacctg
     4201 ttgatggccg aacggctgcg cctctccatt gtggacgagt gccagccgat ttttcttgcg
     4261 attggccggt cggtgtcttt gtcgctcggc gtgttgcgct atccattttc ggtgacgtat
     4321 cccgagttgc taagttggga ccattgcctg gcgctagccg atcatgcgct atatcgcgcc
     4381 aagaaagctg gacgcaattg ctggcaatgc tatcgtccga atgaggccgc tttacgcgtc
     4441 gccatccagt cgcacggaat cgaggaagtt cgccgtctac tgcggatgca caccgaacaa
     4501 gcgttcgaga tgggcttgat tgaggtcgtc gagcaggtca cctctgatgt gcgggtcctc
     4561 tagataggtt ctcgaactgc attgtgttgg cggccgcaga gaacccgctc ttccgaccct
     4621 cagccaccca gcttgcgcac ggcgttctta tggacgaccg ccagacgccg cgcatgaaag
     4681 cttccattca ctaccgtcgc gatagcgtcc atctcagcga gaaatccgtc gtggccgtgc
     4741 gaggtttcga actcggtata atcgcattgc gcaccggcgg cctccagccg gtcggccagc
     4801 gcacgaacat cttttgctgg gaatagccag tcggacgaaa tacccacaag cgagacatgc
     4861 gccttgatgc gttgtaacgc ctccgcttcc gaggcgtatc cccgtcccaa ctcgaaagtg
     4921 tccattgcct tggaaatcac aaggtaggcg ttggcgtcga aacgcgtaac gaacttctcg
     4981 ccctggtggt cgagatagcc ggcgacgtcg tagcgatcgg cgaaggaacg cagcgggtct
     5041 tcgccggtgc gattgggctt acgcgcatag cgctcgtcga acagatcagc cgacttgtat
     5101 gaacacatgc cgatgccgcg cgccaggcct aatccacctg cgggctgctc cccggcggcg
     5161 taccgtccag ccttgaaatc cgggtcaagt tcgatcgcct tgcgctgcag atgattcagg
     5221 gccagcccca tcgccgacag cggcgccgcg ccaatggcga tgcagtgctc cacacgctcg
     5281 ggataatcta ctgtccactg taatgcctgc attccaccaa tcgatgcgcc gatcacgatg
     5341 cgcacgcgct tcacccccag ctcgtccaac acgtgcgcct gggcgcggac gatatcgcga
     5401 atggtgacaa tgggaaaatc aggaccgaaa ggtttgccgg tggcgggatt gatcgaagtc
     5461 ggaccgctgg agccgtagca cgaccccagg atgttggtac agataacgca attgcgatag
     5521 aggtcgaata cgctgccaaa catctcgggc caccaatcgg cggcgcgggc cgagcccgac
     5581 agcgcatgac agatgagcac cgcattgtcg cggcgctgat tcaactcgcc atacacggcg
     5641 tagcgtacgg agaccggctg cagcgagccg ccggcctcaa ggcggaaggg tgcgccgtac
     5701 gcaaacgtca ggtcgccctc ccgcgttggt tctagtgcct cgctcattgc tagtctcccg
     5761 ccacctgggt cgcgaccttc ttacccgtcg cgacctgcag cgcgccgtcg atatcggcga
     5821 tgatatcgcg tacgtcttcg atgcccaccg acaagcggac cagttcaggc gtgacacctg
     5881 tctcgcgctg ttccgattcg ctgagctgct ggtgggtggt cgaagcagga tggataacca
     5941 gcgattttgc gtcgccgatg ttggccaaca ggctgaacag ttgcgtcgca ttgatgaagc
     6001 gcttgcccgc ttcgtatccg cccttgatgc cgaacgtgac cagcgaactg gtcccattgg
     6061 gcaggtactt cttggcccgc tggtagtact tactggtggg caagccggga tagttcaccc
     6121 actcaacatc gggatggctc gacatatact ccgccacctt gagcgcattc tcggaatgcc
     6181 gctccatgcg aagatgcaga gtttccgtac cctgcaacag aagaaacgca ttgaacggtg
     6241 acaggctggc tccagtatcg cgcaggcctt gcacccgcgc cttgaggata aacgccagcg
     6301 gtccgaacgc cccggtgtac gacacaccgt gatacgaggg gtcaggctcg acaaaatcct
     6361 tgaagcggcc cgaggccgcc cagtcgaact tgccgccatc gacgatgacg ccgccgatcg
     6421 aagttccgtg tccgccaatg tacttcgtcg cggaattgac cacgatgtcg gctccccact
     6481 cgatgggacg acacaacgca ggcgacgcgc aggtgttgtc gatgaccagc ggaattccgt
     6541 tttcgtgcgc gatctttgcc agttcctcga cgtcggcaat gtcgagtttg ggattgccca
     6601 acgtctcggt gtacagcgcc ttggtcttgg gtccgatggc cttgcgcagg ccgtcgaagt
     6661 cgtcggcatc cacgaacttc accgtgatgc ccagcttggg aagtgtgtag tggaacaggt
     6721 tataggtgcc gccgtagagc gaagtagtgg agacgatctc atcgccggcg gaagccaggg
     6781 tggtgatgat cagggtttcc gccgcttgtc ccgacgccag cgccagcccg gcgagtccac
     6841 cttcgagtgc ggcgatgcgc ttctcgaaga cgtcggtcgt cggattcatg attcgcgtgt
     6901 aaatgttgcc aaactcctgc agcgcaaaca gtcgcgccgc gtggtcggca tcgtcgaata
     6961 cgtatgacgt cgtctggtaa atcgggacgg cacgcgactt ggttgtggga tcgggatcct
     7021 gcccaccatg gatggcaagg gtgtcgaagt gaaattctgg cttgtcactc atatctactg
     7081 ctcctgttca ggctgaattc gggttcaaaa cgaaacgacc caccgtctgt caggaccgtg
     7141 ggtcgattag gaatctttgc ggctacttac tgttttctat tggccgctcc cacgctcaac
     7201 ccacagtccg gcacagctac acatgcacat ggccatggtg ctgatgcgcg
//
LOCUS       DASRKK010000108         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_397847, whole genome shotgun sequence.
ACCESSION   DASRKK010000108 DASRKK010000000
VERSION     DASRKK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397847"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..2375
                     /locus_tag="VFC15_02405"
     CDS             99..2375
                     /locus_tag="VFC15_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM09043.1"
                     /translation="MSTQAGNLQRPVIKVFVATTVMLTFISFWRAAAIILSDLASSAY
                     YVGGDAEKVIGKSAPWFVLAVMLFSYAVRALYIESSSMFVRGGVYRVVKEAMGGKLAK
                     LSVSALLFDYVLTGPISAVSAGQYLAGFLTDTFHHFGYNIHVPDNSFAALFAIVVCLY
                     FWWKNIQGIHESSERALQIMKITTVMVVLLIVWCLITLAKTGAHLPPFPSAATMHHDH
                     ESLGWLLGTWAARIPLIMMLVGFGHSVLAMSGEESLAQVNREIEHPKLKNLEKTGLVI
                     FLYSLFFTSLVSFFAVMIIPDKVRPDYYANLIGGLAMNVVGPYTLRLFFQGFVVIVGA
                     LILSGAVNTAIVGANGVLNRMSEDGVLTPWFRKPQKKFGTSYRIINLIVGLQIFTIIA
                     SGGNVYLLASLYAFGVIWSFAFKSLAVTVLRYTEPENREWKVPGNFHVKGVEVPVGLI
                     LISLLLFATAIVNLFTKQLATIAGVAFSVAFFAIFTISEHITSKQQTHADSGLDQFNV
                     AQNAELSAETMEVRPGNTLVAVRDPRNLYYLRQILAKTDTTKTDVVVMTARVYHREHS
                     FSGREEVDTSEVFKKYEQELFTAVVAVAEKEGRHVALLVVPTNDIFESIVATAQRLHS
                     SVIVCGLSNQLTADEQGKLTGDAWERLPEPRPRLRLVVVEPKGTKHEYLLGPHLPRMR
                     QEDVDLLHGLWKEVTSDPRYRELHHYHVLNVALQEMSERMHNGAYHDGVMKEIFDEMR
                     SSRGELVADDDVSPPPPT"
     gene            2518..>2892
                     /locus_tag="VFC15_02410"
     CDS             2518..>2892
                     /locus_tag="VFC15_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZM09044.1"
                     /translation="MVRVRFAPSPTGQLHVGNARTALFNWLFARQAGGVMVLRIEDTD
                     LARSEARFEDQLLDDLKWLGIDWDEGPDVGGPHAPYRQSDRLELYREHAERLVKEGKA
                     YLCFCSQEELERDRERALQHQQP"
BASE COUNT          564 a          849 c          856 g          623 t
ORIGIN      
        1 tcaagcactg accgataagc actaaaggca gtacactgat gtactccggt tgcagggttc
       61 ctgcgaaccg aaccctgccc ggggccgcgc caacaactat gtccacacaa gctgggaatc
      121 tgcagcgtcc cgtaatcaag gtcttcgtcg ccactacggt gatgctgacc tttatctcgt
      181 tctggcgagc tgctgccatc attctgtccg atctggcctc gtcggcgtac tacgtgggcg
      241 gggatgcgga aaaggtcatc ggcaaaagcg ctccctggtt tgtgctggcg gtgatgctgt
      301 tcagctatgc ggtgcgggcg ctgtatatcg aatccagttc catgttcgtt cgcggcggcg
      361 tctatcgcgt agtcaaggag gccatgggcg gaaagctggc caagttgtcg gtttccgcgc
      421 tgctgttcga ttacgtgctc accgggccaa tcagcgcagt gtcggctggc cagtacctcg
      481 caggctttct taccgatact ttccatcact tcgggtacaa catccacgtt cccgacaact
      541 cgtttgccgc gctgttcgcg atcgtggtct gtctttattt ctggtggaag aacatccagg
      601 gtatccatga gtccagcgaa cgcgcgctgc aaatcatgaa gatcaccacg gtgatggtgg
      661 tgctgctcat tgtctggtgc ctgatcacgc tggcaaagac aggagctcac ctgccgccgt
      721 tcccttccgc ggcgaccatg caccacgatc atgagtcgct gggctggctg ctgggcacct
      781 gggcggcgcg gattccgctg atcatgatgc tggtgggatt tggccactcc gtgctggcca
      841 tgagcggaga agagtcgctg gcgcaggtga accgcgagat cgagcatccc aagctgaaga
      901 acctggaaaa gaccgggctg gtgatctttc tctacagcct gttttttacc tcgctggtgt
      961 ccttcttcgc cgtgatgatc atccccgaca aggtgcggcc tgattactac gccaacctga
     1021 tcggtggcct ggctatgaac gtggtcggcc cctataccct gcgactcttc tttcagggct
     1081 tcgtcgtcat tgtgggcgcg cttatcctgt cgggtgcggt gaacaccgcc attgtcggcg
     1141 ccaacggtgt actgaaccgc atgagtgaag acggagtgct taccccgtgg ttccgcaagc
     1201 cgcaaaagaa gttcggcacc agctaccgca tcatcaacct gattgttggc ttgcagattt
     1261 tcaccattat cgccagcggc ggcaacgtgt acttgctggc ctctctctat gcctttggtg
     1321 tgatttggag cttcgccttc aagtcattgg ccgtgaccgt actgcgctac accgagccgg
     1381 aaaatcgcga gtggaaggtt cccggcaact ttcacgtcaa gggcgttgag gtaccggttg
     1441 gactcatcct gatttcgctg ctgctgttcg cgacggcgat cgtgaacttg tttaccaagc
     1501 agttggccac catcgccgga gtggccttca gcgtggcgtt cttcgccatc ttcaccatct
     1561 ccgagcacat tacctccaag cagcaaaccc atgcagactc agggctcgac cagttcaatg
     1621 tggcccagaa tgccgagctc agcgccgaga ccatggaggt ccgcccgggc aatacattag
     1681 tagcggttcg cgatccgcgt aacctgtact acctgcgcca gatcctggct aagacagaca
     1741 ccaccaagac cgacgtggtg gtgatgacgg cgcgcgtgta tcaccgtgag cactccttca
     1801 gtggcaggga ggaggtggac acttcggagg tgttcaagaa gtatgagcag gaactgttca
     1861 ctgcggtggt agcagtggcg gaaaaagaag ggcggcacgt tgctttgctg gtagtgccga
     1921 cgaacgacat cttcgagagc attgtcgcca cggcgcagcg attgcactcg tcggtcatcg
     1981 tctgcggctt gtcgaaccag ctcaccgcgg atgagcaagg caaactcacg ggcgatgcct
     2041 gggaacggct gccggaaccg cgtccgcgac tgcgcctggt ggtggtcgag cccaagggca
     2101 ccaagcacga atacctgctc ggtccgcacc tcccgcgcat gcgccaggaa gatgtggact
     2161 tgctgcacgg cctgtggaag gaagtgacca gcgacccgcg ttatcgcgag ctgcaccatt
     2221 accacgtgct caacgtagcg ctccaggaga tgtccgagcg catgcacaac ggcgcttacc
     2281 acgatggggt catgaaggaa atcttcgatg agatgcgcag cagccgcgga gaactggttg
     2341 ccgacgacga tgtttcccct ccaccgccaa cgtaatcgcg gaggcatggg ccgaggataa
     2401 cgcgagaaac aagggcgtct gcgttctcct gaaaatctgt gttcatccgt gcaaatccgc
     2461 ggtcaaacga ttttcttcct cgtctcttgc taacatcgag gggtgaacac cacttccatg
     2521 gtccgcgttc gcttcgctcc ttctcctacg ggacaactgc atgttggcaa cgcccgcacg
     2581 gcactcttca actggctctt cgcccgtcaa gccggcggcg tcatggtgtt gcggatcgag
     2641 gacaccgatc tcgcgcgtag cgaggcgcgc ttcgaggacc agcttctcga cgatctcaag
     2701 tggctcggta tcgactggga cgaaggcccc gatgtcggcg ggccgcacgc accctatcgt
     2761 cagagcgacc gtctggagct gtatcgcgag catgccgagc ggctggtgaa agaaggcaaa
     2821 gcgtatctct gcttctgctc gcaggaggaa ttggagcgcg accgcgagcg tgctctgcaa
     2881 catcagcagc cg
//
LOCUS       DASRKK010000109        24872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_202268, whole genome shotgun sequence.
ACCESSION   DASRKK010000109 DASRKK010000000
VERSION     DASRKK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 24872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24872
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202268"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            610..942
                     /locus_tag="VFC15_02415"
     CDS             610..942
                     /locus_tag="VFC15_02415"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM09045.1"
                     /translation="MQNETGAALRKRRRALLRQLPPLKAILRGSLIERYKRCGKPGCR
                     CAAGPGHGPKYYLSVSYPGLRPRMDYVPQESYAQTAELLANYHRARQILEEICEINRE
                     LLRRREAL"
     gene            1079..3169
                     /locus_tag="VFC15_02420"
     CDS             1079..3169
                     /locus_tag="VFC15_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM09046.1"
                     /translation="MNPKIAEHHRSKPAYIYLRQSTPGQVRHHQESTQRQYALRELAL
                     ELGWSESLIRTLDRDLGKSGTEMAGREDFKTLVADVSMGQVGAVFALEVSRLARSNLD
                     WHRLLELCALTTTLVIDEDGCYDPADFNDGLLLGLKGTMAQAELHFLHARLQGGKLNK
                     AKKGELRFPLPVGFCYDDASRIILDPDEEVRGAVGLVFRLFHETGTAFAVMQSFAASG
                     LRFPKRSYGGAWDGKIIWGRLTHSRVLGMLKNPSYAGMYVFGRYQYRREISPEGRVHK
                     RMHAVAMPDWRVRLKEHHQGYITLEEFLQNQERLEKNRTNGEEMVLAGPAREGLALLQ
                     GLLLCGNCGRALSVRYLGNGGIYPCYQCNWLPREGLASKACMSFRCDLLDAAIAEEVL
                     KALQPAELELALAALQELESRDQTLLRQWQMRLERAQYEAALAERRYQEVDPSQRLVA
                     GTLERRWNDALLQLDEIKQQAAEFQRQEARVATPEQKAKVLALARDLPRLWHAPTTQA
                     KDRKRMLRLLLKDITVEKLAEPKQLLVHIRWQGGACTDLSLQLPPNIADRLRYPAAVI
                     ERVRELAQGLRDAQIAPQLNREGHSSATGKPYTAKIIHWIRWRYQIPPAMLKKPEELT
                     VQQVAQRFAVSLSVVYYWIEHRVIPARQLSSGMQYWITLSENDEQKLQDWVRNSGRIR
                     SGHTSLTAIGGSAS"
     gene            complement(3164..3520)
                     /locus_tag="VFC15_02425"
     CDS             complement(3164..3520)
                     /locus_tag="VFC15_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09047.1"
                     /translation="MIAKAENMPLDKVRVLQRSLYRAAKASPKRKFGVLYDKVCRSDV
                     LRAAYAQVKANKGAPGIDRQSFENIETEVGVETFLNEIRVKLIAKRYKPRPVRRVYIP
                     KADGSQRPLGIPTVLS"
     gene            complement(4645..5883)
                     /locus_tag="VFC15_02430"
     CDS             complement(4645..5883)
                     /locus_tag="VFC15_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZM09048.1"
                     /translation="MSITRRTFLKGGAMAVVGTAAIPSFLTRAVWGAAQAGSNNKRLV
                     VVFQRGAADGLNIVVPHAESSYYAMRPSINIPRQQVIDLNGFFGLHPSLAPFKPLWDQ
                     RHLAIVHAAGSPDTTRSHFDAQDYMESGTPGVKVTSDGWLNRTLQSESPADSPFRAVA
                     LGTSLPRILAGKAPAIAVSNVNGFGIAGGNPAAAPLGNTFEAMYDQSVDSVLHGTGRE
                     TFEAVKMLKSADPQRYTPAPGANYPRGRFGEALRQTAQLIKANLGVQVAFTDIGGWDH
                     HVNEGAAEGQLANVLRDFAQSIAAFWTDLGPLTEDTVLVTMSEFGRTARENGNRGTDH
                     GHANVMFVLGGPVRGGRVYGRWPGLDPSQLNEGRDLALTTDFRRVLGEAVSRHLGNSN
                     LTSVFPGFDTSTQTFLRYLA"
     gene            complement(5895..7985)
                     /locus_tag="VFC15_02435"
     CDS             complement(5895..7985)
                     /locus_tag="VFC15_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZM09049.1"
                     /translation="MGRPHGNHCRNTVSRRLRRVAAVSLLLLAGAGLYATKKNQATAA
                     VDEHRRALNALDRLTFGPRPGDAQTLTAIGVDKWIELQLHPEKIDNTAMQARLAGYRT
                     LTMGSQEMLLEFPPSPVARAAMDGKLPMPRNPYRHAIYVAAIDRIGQQQARKQNKAGS
                     EANVGVTQASAVAASKLVNAAELSQRPQERSEARRIVDQLASSPPDARMQRILSLPVT
                     EQRDLTQGLSFEKRQALFAGFTPEQRETVLALNNPEAVINGEVQSSKLLRAVYSNRQL
                     EEVLTDFWFNHFNVFIGKGADRYLVTAYERDIIRPHVLGKFKDLLVATAQSTAMMFYL
                     DNWQSEGPDSDAARGVPAHGPNPGISGPPGRQGVMYPLPHTPRPQQQPPNPQNNQQKR
                     HDGLNENYARELMELHTLGVNGGYTQQDVTQVARVFTGWSIDEPRKGGGFVYKPRLHE
                     PGQKTVLGHKIKDKGKNEGVQVLEILAHHPSTARFISTELAQRFVSDDPPKSLIDAMS
                     KTFLKTDGDIRAVLETMFHSQEFWAPEAYRARVKTPFEFVVSSLRATQAEVSDPQSLL
                     ATLNKMGMPLYGMQPPTGYSTRADVWVNSAALLDRMNFGLALAANRLPGTTFNLGQLL
                     NDVSVADADPYQVQLKLEQALIAGGISKQTHETIEERIVTPQAMAQFEYPSHPVNVNV
                     IAGLLLGSPEFQHK"
     gene            complement(8304..8612)
                     /locus_tag="VFC15_02440"
     CDS             complement(8304..8612)
                     /locus_tag="VFC15_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09050.1"
                     /translation="MFFGSSREGDTASDFSIKIPQRPIILDQTKTYPRDFDRSLATSA
                     AAEEPIRSILQLHGGRMKETTFWNATHAVFAVEESTDSGRLTGGSEKVQSAGDSECMG
                     "
     gene            complement(8718..8945)
                     /locus_tag="VFC15_02445"
     CDS             complement(8718..8945)
                     /locus_tag="VFC15_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09051.1"
                     /translation="MDNSNLDELQQAYKEAVDQWVAAIRAEEALATPDHSEVAMERWD
                     AAGFGEQDAQNKAKHARDEYKDALRHLHYGI"
     gene            9182..10816
                     /locus_tag="VFC15_02450"
     CDS             9182..10816
                     /locus_tag="VFC15_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZM09052.1"
                     /translation="MTATATPLNSPTPDAPVTAAVHDKINKGPHKIGIFIFAGLLAIG
                     VVYALSNLIADISHVHHPSSAAFLLLGVALLVALGFEFVNGFHDTANAVATVIYTHSL
                     DPHVAVAWSGFWNFLGVVLSSGAVAFAIVSLLPVELILQVGSGAGYAMVFALLSAAII
                     WNLGTWWLGLPASSSHTLIGSIIGVGIANQLMNTRTATSGVDWDQALKIGKSLVFSPI
                     IGFAVAALLLQLSKVFIHDPRLYKAPEGDAPPPFWIRALLVLTCTGVSFAHGSNDGQK
                     GMGLIMLILIGTVPTAYALNHALTAQDSIDFIAVSQQAGNVLDKYVSPDAVVADPRED
                     VKEYVRTKDFTANTMLALRNTVNDVGTEVANFKALGQVPEDRVRNFRNDMYLTSEALR
                     LLKKSGAAKISAADWTVLDNYKKHIDKATKFIPTWVKVAVALALGLGTMVGWKRIVVT
                     VGEKIGKDHLTYGQGAAAEITAMMTIGAADYMGLPVSTTHVLSSGVAGTMWANKSGLQ
                     MSTIRNLLMAWVLTLPASIGLAGLLFWTFNSLMSKG"
     gene            11877..12299
                     /locus_tag="VFC15_02455"
     CDS             11877..12299
                     /locus_tag="VFC15_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09053.1"
                     /translation="MAGETLIPVQPITSIVFALAGFIGVAAFLAGHFGVAAIVPVVAT
                     WGWRAISETLRADYCGASRISAYQIMAFIAVSYLAWVSCLVPCEGPLPNLTAGLTQVA
                     STLVVVVLQACWVLLFLYYGRSRVTASTVTFHVVQGRI"
     gene            12481..14118
                     /locus_tag="VFC15_02460"
     CDS             12481..14118
                     /locus_tag="VFC15_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM09054.1"
                     /translation="MRQILATALMASITFGPLTNPVLASDVTRASQTRNHAAKTATPI
                     KHLVIIFQENVSFDHYFATYPYATNPSGEPAFHAVAGTPTVNGLSNALLNANPNLNPA
                     NGTGATNPFRLDRSQAATTDQDHDYGPEQAAADGGLMDLFPAKTGTAGPPPNPPPSAV
                     TTTGLVMGYYDGNTVTGLWNYAQNFAMNDNSFGTTFGPSTPGALNLISGQTNGVTVQQ
                     NDSGDTVPDGNGGFTLVSDADPVGDTCSGSSQVQMGGTNIGDLLNAAGVTWGFFEGGF
                     DLSITNPNGSTGCSRSHTSTVTGTKKVDYIPHHQPFQYYASTQNLQHTRPTSIAMIGQ
                     QGDAANHQYDSHDFFDAVSAGNFPAVSFLKAPGYQDGHSGYSDPLDEQTFVVDTINFL
                     MSLPEWKDTAVVINWDDSDGWYDHQMGPIVDQSQTSQDALNGPGLCGTAPPALAGITG
                     PHAQGRCGYGPRLPLLVISPWTRSNVVDHTVTDQSSMIRFIEDNWLGGQRIGDGSFDG
                     IANSIDSMFTFVQASTCYRTLILDDQTGEVTQKGCAH"
     gene            complement(14410..14658)
                     /locus_tag="VFC15_02465"
     CDS             complement(14410..14658)
                     /locus_tag="VFC15_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09055.1"
                     /translation="MLAVRAIDDIAIDALDLHCLFERDLHLVHLRPWFVRRILVFMHR
                     NLCEVALADAIRLHVALHDLGKKVRKDEDFARALIGCE"
     gene            complement(14651..14815)
                     /locus_tag="VFC15_02470"
                     /pseudo
     CDS             complement(14651..14815)
                     /locus_tag="VFC15_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(14829..15617)
                     /locus_tag="VFC15_02475"
     CDS             complement(14829..15617)
                     /locus_tag="VFC15_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZM09056.1"
                     /translation="MMDVYWLEQRENDVPAADDWLSESESARLSGLRFAKRRSDWRLG
                     RWTAKCAVAASLKLPDDLQSLKLIEVIAASTGEPEVVLRDNAETATISISHSNGIAIC
                     AVAVGNVALGCDLEAVDARSDAFIADYFTKEEQELIARANGKKRSLLVSLLWSAKESA
                     LKALHVGLRADTRSVDVTKIDALGRWFEVAASDSENTASSRDLANDSAANWTPLDVHC
                     ADGTIFQGWWQHSGNMVRTVVAAPPPTPPIELRCPNRVATTSLL"
     gene            complement(15614..21262)
                     /locus_tag="VFC15_02480"
     CDS             complement(15614..21262)
                     /locus_tag="VFC15_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZM09057.1"
                     /translation="MQREFQRVVIVNRGEAAIRFIQAARDFNREHGTSIATIALYTEP
                     DRHSMFVREADEAVYLGAARIVDPTTNQSKSTYVDFPTLRTAMESARADAAWVGWGFV
                     AEHPDFADLCREMNIVFVGPTSEAMRRLGDKISSKRLAEQAEVPVARWSNGPVETIDD
                     AMRHAERLGYPLLIKATSGGGGHGIRRVNSASQLARAFEGARAEAFKAFGDATVFMEE
                     LVQSARHVEVQVIADDYGTTWAVGVRDCTIQRRHQKVLEEAPSPVLTAAQDLALRQAA
                     VRLSQASGYRNAGTVEFLYEPVSQRFLFMEMNTRLQVEHPVTECTTGLDLVKMQIHVA
                     RGERLEGEPPCTYGHSIEVRLNAEDPENGFAPAPGLIERFRLPSGPGVRVDTGVAVGD
                     AVPAEFDSMIAKIIVYGQSRKEALSRMQRALRESVVVIKGGASNKAFLLELLNCPEVL
                     RATADIGWLDRRSAEGGHLSARYADVALVQAAIESYAADLKLERIQFYASAVRGRPHV
                     RSEVGRTVQLLHRGHSYSLKVCCVGLHRYRVEVDGASIDVRMYSLGQFEYWLTAFGSR
                     FHVVSNVQGLTYRIEVNGVAHSVARDDGGVVHAPAPAVVVSIAVKPGDTVKVGDTLAV
                     LEAMKMEMSVLAPFSGKVRSVTAIPHVQVDTGAPLLQIEPVAGDDESVATTGRVEFGA
                     SRTAGVTREPVRPRWSTNLEELRQLMLGFDVDPAHAMKILSDWSQSAEVAVEYQEIAK
                     SEDEILGIFVDICTLFERQPEMDDLNGGEERSAETYLFSYLHMLETRGKGLPTAFVDA
                     LRRAMVHYGIQTLERSPELEECLLWLCKSHHRVEQQTASIVGLLERHLQRVEASVPRS
                     DEAFRKLLGRLIHTTRGLFPTVSDLAREVRYRCFEHPVFERARRQIYAQAEEHLAYLA
                     ANPNAADLRQRVGALIECPQPLAGLFSGRYAAAPPALRELMLEIETTRYYRGQTLSKF
                     RTVPVNDRTCVTAEYDDNGKRVHIFSLNTEFLGLADAARALFRGIDEVPVDHEIVLDF
                     YAWNPEFLSDPELIEKEVRSTLNQVGFPRTIQRIVVAIGGPGRGPSMAGMQHFTYRPS
                     ASLYEEELVVRGVHPMMAERLHLWRLNNFKVERLPSVEDVYLLYAVANDNPKDERLFA
                     VAEIRDLTPVRDQEGRIAQLPHLERMFGEAVAGMRMFQSRRSPNKRLHWNRILLYVWP
                     TVNFTRKEFDQIVRKLAPSTDGLGLEQVVVRTRIPQPDTGRVRDMIVRISSPGGAGQI
                     MTIRPASKQQPLTPLDDYTQKVVRMRQRGMIYPYEIVKMLTPVPEGIATQFPPGDFVE
                     YDLDADGHLVPVNRPYGKNTSNIIVGVLRNFTTKYPEGMSRVVLMGDPSKDLGAIAES
                     ECRRILAGLDLAQEKGIPLEWFTLSAGAKISMESGVENMDWIAKVLRRLIEFTQAGCE
                     VNLVVNGINVGAQPYWNAEATMLMHTRGILVMTPKAAMVLTGKRALEYSGSVSAEDNE
                     GIGGYDRIMGPNGQAQYWARNIDEACHILLRHYDHTYLAPGERFPRKAVTTDPIERDV
                     RLYPHTHGGEGFATVGEIFSDQTNPGRKKSFDIRKVMRAVMDQDQLPLERWSGMRAAE
                     TGVVWDAHLGGYPVCLIGIESHPAPRLGFVPADGPDQWTAGTLFPLSSKKIARAINAA
                     SNNRPVVILANLSGFDGSPESLRKLQLEYGAEIGRSVVNFKGPMVFCVISRYHGGAYV
                     VFSGALNQSLEVAALEGTFASVIGGAPAAAVVFASEVEARTKKDPRLQALTETMNKAM
                     TKADGVEKNRLRAQWNELFKVVHSEKLGQMAEEFDRVHSVNRALEVGALHHILPPVDL
                     RPYLIHAVESGMARERELHSAKLQSESSGTIHAATPTLVS"
     gene            complement(21308..>24872)
                     /locus_tag="VFC15_02485"
     CDS             complement(21308..>24872)
                     /locus_tag="VFC15_02485"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM09058.1"
                     /translation="RILTLMAEKTGYPKDMLDLELDLEADLGVDTVKQAEMFAAVREI
                     YNIPRDENRKLRDYPTLAHVIRFVFENRPDLAGTTAPTPAPIKVAATTAPVAPVVATA
                     PVPAAPTVSATVVAARAPVAAVQPVAETESVTERILTLMAEKTGYPKDMLDLELDLEA
                     DLGVDTVKQAEMFAAVREIYNIPRDENRKLRDYPTLAHVIRFVYESRPDLAPGVSPKP
                     VIVQPVATVAPAATSQLVSDDAIRVKVLEIVAEKTGYPKDMLDLDLDLEADLGVDTVK
                     QAEMFASVRAAYNIPREENMKLRDFPTLAHVIQFARDKQNLGVTPAASAPAAVAPPAA
                     VKHVARPRPALASFDAANLIPRRVPVPTLRPPLTICKPTGVKLGPGSRVVVMPDKGGV
                     VTVLTQHLRTLGVEALVLDAALDANALTERLKSWLSAGPIQGVYWLPALDNEGDLQHM
                     NLAGWHEALRVRVKSLYTTMRALYEQVATPGSFLVSATQLGGQHGYDAAGAVAPLGGA
                     VVGFTKTYKRERPEVHVKAVDFEAGRSASEVAVAMIEETLRDNGAVEVGYKNGLRWAV
                     GLQEQPVTEGGHGLMLDQDSVFVITGAAGSIVSAITADLAAASGGTFYLLDLVPEPDP
                     NNPDLARFVSDKDGLKRDLFERIKARGERATPALVEKELVGLERAHAAQSAIDAVRSA
                     GGTPYYFSINLTDADGVTNIIKQVRQRSGRIDVLLHAAGIERSHFLPEKDQREFDLVF
                     DVKSDGWFNLLHAIGDMPLGATVAFSSIAGRFGNGGQTDYSAANDLLCKITSSFRTMR
                     PGTRGIVIDWTAWGGIGMATRGSIPKMMELAGIDMLPPEAGVPMIRRELTSADNRGEL
                     VVAQRLGIMLNEWDETGGLDTKALAAALEQRVPARGPMVGTVTGMGLYSGLTFETTLD
                     PKAQPFLYDHRIDGTPVLPGVMGIEAFAEAALCMHPGWSVEAIEEVNFMAPFKFYRDE
                     PRTVFIQAQFYSDGDNLRAECRLIGRRTLPNQSQPQETVHFTARVRVTKQPHQASAAV
                     PLKLTSEGIVDAAQIYRIYFHGPAYQVLDHAWRDGDRIVGQMSETLPSDHDPSQGPTL
                     IGPRLIELCFQTAGLIEISEHNRMGLPLFVEQVSWSRAPEVADGPLFAVVTPDAAGEK
                     FDAEVVDKQGNRYMRLTGYQTVTLPDSVDAEPLQALQHVIA"
BASE COUNT         4943 a         7423 c         7250 g         5256 t
ORIGIN      
        1 cgcagtccgt atgatgccgt acagcgcgca tcaagagttc ccagtcgatc tcatcaaaga
       61 aactcctgat gtcaagatcg agcacccagt catgtcgcca gcatcgctgc cgcgccacag
      121 ccagtgcatc atgcgctgac ctgcccgggc gatatccata agagtcggca tggaacacgg
      181 gttccagtat cggctccaga tatctttgga ccaccgtctg agcgatgcga tcggataccg
      241 tcgggacggt cagcgcaaac tgcacttgaa cccagtgagc ctttgggatg ggttgagatc
      301 cgtcatccct tccatccgct tcgtggtcaa cgcttccctg tgctcaagag gcgacgcgta
      361 gccggcgttg atactctgat tcttcgcgag ttgaagcgcg gcaccttcag cgtggcacgg
      421 gaatggaccg attgggcgga tccgccgacg tacagctcgc tggggttacc gccggggcga
      481 ctcgatattg attcacttct tgaattagtt actttcttgg aacaacttac ccatgccttc
      541 acacaaaggt tgactccatg agttccgcaa taatattatc agaccagtta tacacgtggt
      601 ctgacataca tgcagaacga aaccggtgcc gcattgcgaa aacgtcgcag agctcttctg
      661 cgccaactgc ccccgctcaa agccatcctg cgcggctccc tcatcgagcg ctacaaacgc
      721 tgtggcaagc cgggatgccg atgcgccgcc ggtcccggtc atggccccaa gtactatctt
      781 tcggtgagct acccaggatt gcggccacga atggattacg tgcctcagga gtcttatgct
      841 cagaccgcag agctcctcgc caattaccat cgagcccgcc agatcttgga ggagatctgc
      901 gagatcaacc gcgaactgct gcgccgtcga gaggcgctct aaagggtcgc catgagcaga
      961 gcaacttccg tcctccaagc gacgatcgat gtgggattgg gcggcgtgct cctcgccaat
     1021 atgctcgcag ggtggctcga cggagaccca aaggttttgc caactgcgga gaccgccgat
     1081 gaacccgaag atcgcagaac accaccggag caaaccggct tacatttatc tacgccagtc
     1141 aacgccgggc caagtacgcc accatcagga gagcacgcag cgccagtatg cactgcgcga
     1201 gttggcactg gaactgggct ggagcgaatc cctgatccgc accctcgacc gggacttggg
     1261 aaagtctggg accgaaatgg cggggcgaga ggacttcaag accttggttg cggatgtctc
     1321 catgggccag gtgggcgcgg tgtttgcctt ggaagtctcg cggttggccc gctccaacct
     1381 cgactggcat cgactgctgg agctgtgtgc gctcaccaca accctggtga tcgacgaaga
     1441 tggttgctac gatccggcgg acttcaacga cggattgttg ctcggattga aggggacaat
     1501 ggcgcaggcc gagttacatt ttttgcacgc tcgcctccag ggtggcaagc tcaacaaagc
     1561 caagaaaggc gagttgcgtt tcccccttcc ggtcggtttt tgctacgacg acgccagccg
     1621 tatcatcctt gatccggatg aagaagtgcg gggcgcggtg ggcttggtgt ttcgtctctt
     1681 tcacgaaacc ggcaccgcct tcgccgtgat gcaaagcttc gcggcaagcg gtttacgctt
     1741 tcccaagcgg tcctatggtg gggcctggga tggcaagatc atatgggggc gcctgacgca
     1801 cagccgggtg ttgggcatgc tgaagaaccc ctcctatgca gggatgtacg tctttggtcg
     1861 ttatcagtat cgtcgtgaga ttagcccaga gggcagggtt cataaacgga tgcacgcggt
     1921 cgctatgcct gactggcgtg tccgcctgaa agagcaccac caggggtaca tcactttgga
     1981 agagttcttg cagaaccagg agcgcttgga aaagaaccgc accaacggcg aggagatggt
     2041 actggccggc ccagcgcgcg aaggcttggc tttactccaa gggctactgt tgtgcggcaa
     2101 ctgtggtcgc gccctttcgg tacgctatct gggcaacggc ggcatttatc cctgctatca
     2161 atgcaactgg ttaccccgcg aaggtttggc tagcaaggcc tgcatgagct tccggtgtga
     2221 tctgctcgat gccgccatcg cggaggaagt gctgaaggca ttgcagccgg ccgagctgga
     2281 gctcgccctg gcagccctgc aggagttgga gtcgcgcgat cagaccttgt tgcgccagtg
     2341 gcagatgcgg ctcgaacgtg cccaatatga ggccgcgctc gccgagcggc gctatcaaga
     2401 agtcgatcct tcccaacgtt tggtggcggg cacactggag cggcgctgga acgatgcgct
     2461 gcttcaattg gacgagatca aacaacaagc cgcggagttc cagcgtcaag aggctcgcgt
     2521 cgctaccccc gagcagaagg ctaaagtcct cgctctagca agggatttgc cgcgtttgtg
     2581 gcatgctccc accacacaag ccaaggaccg caagcggatg ttgcggctgc tgctcaagga
     2641 cataacggtg gaaaagttag ccgagcccaa gcagctcctg gttcacatcc gctggcaggg
     2701 cggcgcctgc accgatctct ccctgcagct cccacccaac atagcagacc ggttgcgcta
     2761 ccctgccgcc gtgattgagc gagtgcgcga gctggctcag ggcttacgcg atgcgcagat
     2821 cgcgcctcag ctcaatcgag aaggtcatag cagtgccacg ggtaagccgt acaccgcaaa
     2881 gattatccac tggatccgtt ggcgctatca gatcccgccg gcaatgctca agaaacctga
     2941 ggagttgacg gtgcaacaag tggcgcagcg ttttgcggtc agtctctccg tcgtgtacta
     3001 ctggatcgag catagagtga tcccagcgcg tcagcttagc tcgggaatgc agtattggat
     3061 cactctgagc gaaaacgatg agcagaaact gcaagattgg gtacgcaact ccggcagaat
     3121 ccgatctggc catacatccc taaccgcgat tggaggaagt gcatcatgaa agcaccgtcg
     3181 ggataccgag cggtcgttgg ctcccatctg ctttggggat gtaaaccctt cgaacaggcc
     3241 ggggcttgta ccgcttcgcg atgagtttga cacggatttc gttcagaaaa gtctcaaccc
     3301 cgacttccgt ttcgatgttc tcgaacgatt gtcggtcgat gcctggggca cctttgttgg
     3361 cctttacctg agcgtacgcc gcacgcaaaa catcgcttcg gcagaccttg tcatacaaca
     3421 ctccgaactt gcgcttcgga ctcgccttgg ctgcacggta tagcgaccgt tgcaatactc
     3481 gcaccttgtc tagcggcatg ttttcagctt tcgcaatcat gcagtgcctc tcgcatcgat
     3541 tcgcttggca gaagcagagg cccttccctc cggtgaggtt ttgttgtccc cgccatcatc
     3601 ggtagtacgg cctcctccga ttccttatcc ccgcatcgct ccgactttcg gcgcatacct
     3661 tatgccttca cttacgcggt ggcttaatcc gcgacggggt aaggtttcca tagttccgct
     3721 cagccctgtg catgcatccc atcctctata ccccgagttc ctaccctggc ttcgaatggt
     3781 tcgttcatcc accagagatc gcggccttcg tccccgttct acgcactcgg cggaaccacc
     3841 cttcactgtg ctctcctagc atggctgcac agctgaaatt aacgaggctt aacagaggtt
     3901 cacttacgtt acgggctgta tgcttgcgcg tacccactca ctaggtctgg ggccacgttt
     3961 atccggcgtt gctcccgcgc ttcagtcgcc ctccacgcgt acgccatagc tacgcaggca
     4021 acgaaccgac tcctgcgaca ggactttcac ctgcaagtgg ctgagcagct tcctatgcgt
     4081 tcaaaacggt acatcttggt tcgtcacggt tgtctcgtcg gacacacttt cggcgggatc
     4141 cccatggccg tttccgtttc ctcgggaatc gaggaactca atcccgtcgg catagatgag
     4201 gtccctgtaa tgcttgtcgc aagtccgttt gtcgtcccaa gactcggtgc acttccgacc
     4261 ctccacgtat atgcgggatc ctttgcggag gtgttcgccg agcaggcttt caggatccag
     4321 ccggataacg agccactcgg aagttagctt cactcgacaa aaacagggct tcgggacaaa
     4381 ttccgggacc accttccgct cgggtcgctg ttctacagcc gatgagtcgc atgctgaacc
     4441 gcatgtgctg gtaactggcc gcctcttcgt ggctcgggag ggagctggct ccggatggtt
     4501 agccacactc cgaaccacct cagtccacaa caccggttgg ctcgtagctc gggaacgatc
     4561 agggaagtgc agctaacacc gtgtattact tggcactctc tgcgacgcaa agactcgttc
     4621 ccgtcgtcgt cgaagggtgt tgcgtcacgc cagatatcgc aaaaaggtct gggttgaggt
     4681 gtcaaagccg ggaaacacac tggtcaagtt ggagttgccg aggtggcgag aaacggcctc
     4741 gcccagcact cgccggaaat cggtagtgag cgcgaggtcg cgtccttcgt tgagttgcga
     4801 tgggtcaagg cctggccagc gcccataaac ccggccgccg cgcaccggcc cgcccaggac
     4861 gaacatgacg ttggcatgcc cgtggtcggt accgcgattg ccgttttcgc gtgcagtgcg
     4921 tccgaactct gacatggtca cgagcacggt gtcttcggtg agggggccga ggtcggtcca
     4981 gaaggcggcg atggactgcg cgaaatcgcg aagcacattc gcgagttgac cttccgccgc
     5041 accttcgttg acatgatggt cccacccacc gatgtcggtg aaggcaacct gtacgccaag
     5101 gttggccttg atgagttgtg cagtctgccg gagcgcttcg ccaaatcggc cccgcggata
     5161 gttcgcaccc ggagccggcg tatagcgctg cggatcggcg gacttcaaca tcttcactgc
     5221 ttcaaacgtc tcgcggccgg taccgtgcaa cacgctgtcc accgactggt cgtacatggc
     5281 ttcgaaggtg ttgcccagtg gcgcagcggc tgggttcccg ccggcaatgc caaatccatt
     5341 tacgttgcta acagcaatcg caggggcttt gccggccaga atgcgcggca gtgaggtgcc
     5401 gagcgcgacg gcgcgaaacg gactgtcagc gggagactcc gactgcaagg tgcgattgag
     5461 ccagccgtcg ctagtgacct tcacgccggg cgtgccggat tccatgtagt cctgcgcgtc
     5521 gaagtgtgag cgcgtcgtgt cgggtgagcc cgcggcatgg acaatcgcga gatgccgctg
     5581 gtcccatagt ggcttgaacg gtgcgagcga gggatgcaac ccgaagaagc cgttgaggtc
     5641 gatcacctgt tgccgaggaa tgttgatgct gggccgcatg gcgtaatagg aactctcggc
     5701 atgaggcaca acgatgttca ggccgtcggc ggcgccgcgt tggaagacga cgacaagccg
     5761 cttgttgttt gatccggctt gggccgcgcc ccaaactgcc cgcgtaagga aggaggggat
     5821 tgccgcggtg ccgacaacag ccatcgcacc gcccttgagg aatgtccgtc gcgtgatgct
     5881 catgtgttga tctcttactt atgctggaac tctggtgagc ccagcaacaa tccggcaatg
     5941 acgttaacgt tgaccggatg cgaaggatac tcgaactggg ccattgcctg cggagtgacg
     6001 atgcgctcct cgatcgtctc gtgcgtctgc tttgaaatgc cgccggcaat gagcgcctgt
     6061 tcgagcttca actgcacttg atagggatcg gcgtcggcta ccgacacgtc atttaatagc
     6121 tgtccgagat tgaaggtggt gcccggcagc cggttagcgg cgagggcgag cccgaagttc
     6181 atgcgatcga ggagcgcggc cgagttcacc cacacgtctg ccctggtgga gtagccggta
     6241 ggcggctgca ttccgtacaa cggcatgccc atcttgttga gcgtagccag cagcgactgc
     6301 ggatcgctga cttcggcttg ggtggcgcgc agcgacgaca ccacaaattc aaacggcgtc
     6361 ttcacgcgtg cgcgatacgc ttcgggagcc cagaactcct gcgagtggaa catagtttcc
     6421 agcactgcgc gaatgtcgcc gtcggtcttc agaaaggtct tcgacattgc gtcgatcagg
     6481 gacttcggcg gatcgtcgga gacgaaccgc tgcgccaact cggtggagat gaaacgcgca
     6541 gtggaaggat ggtgtgcaag aatttcgagc acctgtacgc cctcattctt acctttgtcc
     6601 ttaatcttgt gccccagtac ggttttttgg cccggctcgt gcaagcgcgg cttgtaaacg
     6661 aatcctccgc ccttgcgcgg ctcgtcgatg gaccagccgg tgaagacgcg ggccacctgc
     6721 gtaacatcct gctgggtgta gccaccgttg actccgagag tgtgcagctc catcaactct
     6781 cgagcgtagt tctcgttcag tccgtcgtga cgtttctgtt gattgttctg ggggttcggc
     6841 ggctgttgct gcggacgtgg ggtgtgaggc aaagggtaca tcaccccttg gcggcccggg
     6901 ggcccgctta ttccagggtt tggcccgtgt gcgggaactc cacgcgccgc gtccgagtcc
     6961 ggaccctcgc tctgccagtt gtcgagatag aacatcatcg ccgtactttg ggccgtcgcg
     7021 accagcaggt ctttgaactt gcccagcaca tgcggacgga taatatcgcg ctcatacgcc
     7081 gtgaccaggt agcggtcggc tcccttgccg atgaagacat tgaagtggtt gaaccaaaag
     7141 tcggtgagca cctcttcgag ttggcggttg ctgtaaaccg cgcgcagcag cttggatgac
     7201 tggacctcgc cattaatgac cgcctcagga ttattgaggg ccagtaccgt ctcgcgctgc
     7261 tcgggagtga aacctgcgaa cagcgcttgc cgcttctcga aagacagacc ctgagtcagg
     7321 tcgcgctgct cggtcaccgg taaggacaga attcgttgca tgcgtgcgtc tggcggcgag
     7381 gaagcgagtt ggtccacaat gcggcgagct tcactccgtt cctgggggcg ctgtgaaagc
     7441 tctgcggcgt tcacgagttt gctggctgcc acggcgctgg cctgcgttac tccgacgtta
     7501 gcttcggagc cagccttgtt ctgttttctt gcctgttgct gcccgatccg gtcaattgca
     7561 gcgacgtaga tggcgtggcg ataagggttg cgtggcatcg gcagctttcc gtccatcgcc
     7621 gccctggcca ccggactggg cggaaattcg agcagcatct cctggctgcc cattgtgagc
     7681 gtccgatagc cagcgagtcg cgcctgcatg gcggtgttgt caatcttttc aggatgtagt
     7741 tgcagctcaa tccacttgtc cacgccaatc gctgttaggg tctgcgcatc accgggccgg
     7801 gggccgaaag taaggcgatc gagcgcgttt agcgcccgcc tatgttcatc aaccgcggct
     7861 gtagcctggt tcttcttcgt cgcgtacagg ccagccccgg ccagcagcag aagggacacc
     7921 gcggcaacac gacgcagccg acgactgact gtatttcggc agtggttgcc atgtggccga
     7981 cccatgaacg tgcgctccgt tcgaatgact tagtctttat aaacctgaag ggaacgaaaa
     8041 gttgttgaaa cgctatacag gtatggcgag ctgtcgtacc gaactcgcga atgcgcttga
     8101 ttcaaggaga aaacggaccg attgtcgata tagcgggagt cgtccgcgat tttcccatac
     8161 tcggatggat cccggacgag cgcgtaccgt ttgctgaccg tcgggggagt caaaccggct
     8221 ttgacgagat tggaacgcca cgagtgctgt aacaccacgt atcctacaac aagacttcgg
     8281 gcccgcgtcg acggaagagc gtcctagccc atacattcag agtcgcccgc cgattgcacc
     8341 ttctcgctgc caccggttag tcgaccggaa tctgttgatt cttcgacggc gaacacggcg
     8401 tgggtcgcgt tccaaaatgt ggtctccttc attcggccac catgcaactg gagtatggag
     8461 cggataggct cctcggctgc cgcacttgtc gccagggagc ggtcaaaatc ccgaggatac
     8521 gtcttggttt ggtccaggat gattgggcgc tgaggtatct tgatggaaaa atctgaagcg
     8581 gtgtcgcctt cccgcgacga accaaaaaac aaccggactc caacatttcg catgggaatc
     8641 gaggcatagc gatcatggac ggacggagtg ccggtggccc tagaagactg actccatgca
     8701 ctagccgtgt accagactca aattccgtag tgcagatggc ggagagcgtc tttgtactca
     8761 tctcgcgcat gcttggcttt gttctgcgcg tcttgctctc caaatcctgc agcatcccag
     8821 cgctccattg cgacctcgga gtggtcaggc gtggccagag cttcctccgc gcgaatggca
     8881 gctacccatt gatcgaccgc ttccttatag gcttgctgta gttcatcgag gttcgagttg
     8941 tccatagatg tcggatacta tggcagcctt tgcagccggt caatcatagt acaccttcat
     9001 ttacccaaaa ttaatctgtc tcgcaacccg caccgatgac tgtgggacaa ttgccggtca
     9061 caaaacattc accaggcatt caattgctgt taatgatgac ggccgccgcc gcatagtacc
     9121 ttacaccgca cgggcgcgcc tgcccttcgc cttcaactct cgctttcaag aaggacaatt
     9181 catgacggct acagcgacac cgctgaattc cccaacgccg gacgcacccg tcaccgcggc
     9241 ggtacacgac aagatcaaca aaggcccgca caagatcgga atcttcatct ttgcggggct
     9301 gcttgcaatt ggcgtcgtgt atgcgctctc gaacctgatc gcagacataa gccatgtgca
     9361 tcatccttcc agtgccgcat tcctgctgtt gggcgttgcg ttgctggttg cgctgggatt
     9421 cgagttcgtc aacggttttc acgatacggc caacgcggta gcgacggtca tttacacgca
     9481 ttcactcgat ccgcacgtag cggtggcatg gtcaggattc tggaacttcc tgggcgtggt
     9541 gctgtcctcc ggcgcggtcg cgttcgccat cgtgtcgctg ctgcctgtgg agttaattct
     9601 gcaggtgggc agtggcgcgg gatatgcgat ggtgttcgct ttattatcgg cggccatcat
     9661 ctggaacctg ggtacctggt ggctggggct tccggcctcc agctcacaca ctctgatcgg
     9721 ctcgatcatc ggagtgggca tcgccaacca gttgatgaac acgcgtaccg caaccagcgg
     9781 tgtggattgg gaccaggcgc tgaagatcgg caaatcgctg gtgttctccc cgatcatcgg
     9841 gttcgcggtg gcggccctgc tgctgcaact gtcgaaggtg tttatccatg atccgcggct
     9901 gtacaaggcg cccgaaggtg acgcaccgcc gccgttctgg attcgcgcgc tgttggtgct
     9961 gacctgtacc ggcgtgagct ttgcgcatgg ttccaacgac ggacagaaag gcatggggct
    10021 gatcatgctc atcctgatcg gcaccgtacc aacagcctac gccttgaacc acgctctcac
    10081 cgcgcaggac tcgattgact ttattgcggt ctcacagcag gcgggaaacg tgctcgacaa
    10141 gtatgtgagt cccgatgccg tggtggccga tccgcgcgaa gacgtgaagg aatacgtgcg
    10201 caccaaggac ttcactgcca ataccatgct ggccttgcgc aacacggtga acgatgtggg
    10261 aaccgaagtg gcaaacttca aggcattagg gcaggttcca gaggaccggg ttcgcaattt
    10321 ccggaatgac atgtacctga ccagcgaagc gctgcgtctg ctgaagaaat cgggagcggc
    10381 aaagatttcg gcggcggatt ggaccgtcct ggacaactac aagaagcaca tcgataaggc
    10441 cacgaaattc atccccacgt gggtgaaggt cgcggttgcg ctggcgctcg gcctgggcac
    10501 gatggtcggc tggaagcgca tcgtggtgac tgtgggcgag aagatcggca aagaccacct
    10561 gacctacgga cagggcgcgg cagccgaaat caccgccatg atgaccatcg gcgccgccga
    10621 ttatatggga ctgccggtga gtaccaccca cgtattgtcg tcgggcgtgg ctggtacaat
    10681 gtgggccaac aagtcgggcc tgcagatgtc aacgatccgc aatttgctga tggcctgggt
    10741 gctaaccttg ccggcgtcga tcggcttggc cggcctgctg ttctggacgt tcaatagctt
    10801 gatgagcaag ggataggaag caactggcag ttgacgtggc gcgacagacc ccatcactca
    10861 tcggactgtt gagtgtcgtc tgcagccgag ttccgcgcaa cgccctatgg cgccgaaatc
    10921 ggtcatcgct gggccggtcc aaacgatcgc cacattggcg aaaccgcgag tagtcataac
    10981 tatccagata ccgtctcgaa ttcacctgta atttaggctc tgcacaagaa tctgaaagag
    11041 tgggcatttt aggcggcttt tgcgaaagtc cctcgacgga tgtcagagcc tggaagaact
    11101 cgttcttcga gtggcactcc gataggtgga tttgcccgca aactgatgaa gggtcttggg
    11161 aatagccggt acttggcctg cgagcatcga caggtaagct actgattcaa tgatgggtta
    11221 acggccttta atggtagaaa gtgcaatcaa ctgggcggtc gaaatcctaa cgcaagaccc
    11281 gccgccaagt tcgacgaagt gctggcggat taccactcac gctggcccca accgggatat
    11341 cgaggctgga tcggctaaca aattactccc tgccctagtc ggcaaagatc ctattgaagg
    11401 accgataaac actggagttg agttgcttca ccggttttag ctacccgcaa ccgtttcagg
    11461 accccggatg gcgacagcca gtaaaaatgc gggcctgttc agtttcctgc acagaaccgt
    11521 aatttacagc tggtttatca agccacgcca atattttggg tatcactcta atgactgtgc
    11581 tggtgtcgtc ggtgggcaca ccgctgatcg ctgctgcggt tctgatcttc gggatgatgc
    11641 ttatttgtgt gccctcgtca acgatcgtcg cgatttccat ttatgttttg ccattactgt
    11701 cggcagcgtc gatctgctac gcactgtcgg agtcgattgg ccggttagcg tgcctgacct
    11761 tcggatgctg ctaaggcgtc ccgctgcgcg acgccagtcc gcgactggct agactgttca
    11821 ggcgccacta tgttgtattt catggcgaca cgaagaaagc tgcatacgct tctggattgg
    11881 ccggagaaac tctgatcccg gttcagccga tcacgtcaat cgtattcgcc ctggccggct
    11941 tcattggagt tgcagcgttt cttgccggac actttggagt agcggcgata gtgccggtcg
    12001 ttgcgacctg gggttggcga gcaatctcag aaactctgcg cgccgattat tgtggtgcct
    12061 cacgcatttc ggcataccag atcatggcct ttatcgcggt ttcgtacctg gcgtgggtat
    12121 cgtgcctggt tccctgcgaa ggtccgctac ccaatctgac ggcggggctg acgcaagttg
    12181 cgtcaaccct tgtcgtcgtt gttttgcaag cgtgctgggt gctgctattc ctctactacg
    12241 gccgcagccg agtgacggcg tcaacggtta cgttccatgt agtgcaaggc cgaatctaat
    12301 cagcaacacc acgttcctac tcgctccggc tccctagttt tcaatctccg gctcttagtg
    12361 ctcttcctag cgtcaccaaa cattaattca tgatctgttg aagtcagagt caccgctgat
    12421 tgacatgggc tctgtaaaaa ggtggacagt agggctccca tcctatgaac ggagtgtgct
    12481 atgcgacaaa ttcttgcgac tgccttgatg gcgtccatca ccttcggacc gctaaccaat
    12541 cctgtgctgg ccagcgacgt cacgcgggct tcccagacac gaaaccatgc cgccaaaacg
    12601 gccacaccca tcaaacacct ggtgatcatc ttccaggaga acgtctcctt tgatcactat
    12661 ttcgcaacgt atccctacgc tacaaatccc tcgggagaac cggcgttcca cgcagtagcc
    12721 gggaccccaa cagtgaacgg cctcagcaac gctttgctga acgccaatcc aaatctgaat
    12781 ccggcgaatg gaaccggcgc tactaatccg ttccggcttg atcgttcgca agctgcaacc
    12841 accgatcagg accacgacta cggtcccgag caggccgccg ccgatggcgg attgatggac
    12901 ctgtttccgg cgaagaccgg aaccgccgga ccgccgccaa atcctccgcc atcggcggtg
    12961 accacaaccg gcttggttat gggctattac gacggcaaca cggtgaccgg gctgtggaat
    13021 tatgcccaga acttcgccat gaatgacaat tcctttggca ccacgttcgg tccttcgacg
    13081 cccggagctc ttaacctgat ttccggacag accaacggcg ttactgtgca gcagaatgat
    13141 agtggcgaca ctgtgcccga cgggaatggc ggctttactc tggtcagcga tgccgatccg
    13201 gtgggagata cgtgctcggg cagttcgcaa gttcagatgg gcgggacgaa tatcggcgac
    13261 ttgctcaatg ctgcaggcgt gacctggggc ttcttcgaag gcggctttga cctcagcatc
    13321 accaatccga acggcagcac gggttgcagc cgctcccata cgtctaccgt aacggggacg
    13381 aaaaaggtcg attacattcc gcaccaccag cctttccagt actacgcttc gacgcagaac
    13441 cttcaacaca cacggccaac gtcgatcgcg atgattggcc agcagggcga tgcggccaac
    13501 caccaatacg acagccacga cttcttcgat gcggtttcgg cggggaactt cccagcggtc
    13561 agttttctga aagctccggg ctaccaggac ggccattcag gatactcaga tccgctcgat
    13621 gagcagacgt tcgttgttga caccattaat ttcctgatga gtcttcctga gtggaaggat
    13681 actgccgtgg tcatcaactg ggatgactcg gacggatggt atgaccacca gatggggcct
    13741 atcgttgacc agtcacagac ctcgcaggac gccctgaacg ggccagggct ttgcggcacc
    13801 gctcctccgg ctctggctgg cattaccggg ccccacgccc aaggccgatg cggttacggt
    13861 ccgcgactgc ctctcttggt gatttcgccc tggactcggt ccaacgtggt cgatcacacg
    13921 gtcacggacc agagttcgat gatccgtttc atcgaggaca actggctcgg tggccagcga
    13981 atcggtgacg gctcattcga cggcatcgcc aattccatcg actcgatgtt caccttcgtc
    14041 caagcgtcga cgtgttaccg cacgctgatc ctggacgacc agacgggcga ggtcactcaa
    14101 aaggggtgcg cccactagga tttccctacc ggccgaccct ccgtcaagaa agcgcaggca
    14161 tatgctcccg tggggcatgt gcctttgtgc tttaagggtt tgtcacattg gccctaaact
    14221 ggcttggagg agatgttccg cggcaggatc tccgcatgca gcggaacttc gagccgatgc
    14281 gtcggcaggt cgaaggaagg cggcagatcc ttctccgaca acccagcgaa gctaatcatc
    14341 taccgggcgc tcgtggaaac gcaatttgat gcgcccaagt acctcgtgct cggctaagtc
    14401 ctcggataac tactcgcacc cgatgagcgc gcgggcaaag tcttcgtcct tcctcacctt
    14461 tttgccgaga tcatgcagtg ctacatgcag gcgtatggcg tcagcgagag cgacctcgca
    14521 cagattgcgg tgcatgaata ccagaatgcg acgtacaaac catggccgca gatgaacaag
    14581 gtgcagatca cgctcgaaca ggcaatgaag atcgagggcg tcaatcgcta tgtcgtcgat
    14641 ggcgcgaacc gccaacaaag gtggttgttt tccaatgacc gtgcggagcc gaagcgatgc
    14701 aacgcaggcc cctgggtgcg cggccatagc gccgcgtcat gctggtcgat gtccaggttt
    14761 catcaataaa caccagcttg gtgacgtcta tgaccggttg agcttcactc catgcgcgca
    14821 caaacttgct aaagcagcga tgtagtagca acccggttgg ggcatcgcaa ttcaattggc
    14881 ggagttggcg gcggagcggc gactacggtt cgcaccatgt tccccgaatg ctgccaccat
    14941 ccctggaaaa tcgtcccgtc cgcacagtgg acgtctaaag gcgtccagtt ggcggcggaa
    15001 tcgttcgcca gatcacgcga actggcggtg ttttccgagt ccgacgcagc gacttcgaac
    15061 cagcggccca gagcatcgat cttcgtgacg tccacgcttc gggtatcggc acgcaatcca
    15121 acatgcaagg ctttcagcgc gctctccttc gcgctccaca gcagcgacac caacagggac
    15181 cgctttttgc cattggccct ggcaatcagt tcctgctctt ctttggtgaa gtaatcggcg
    15241 atgaaggcgt cgcttcttgc atcgacggcc tccagatcac aacccagcgc cacgttaccg
    15301 acggcgacgg cacaaatggc aatgccatta ctgtggctaa tcgaaatcgt ggccgtctcc
    15361 gcattgtcgc gaaggaccac ttcgggttcg cctgttgaag ctgcaataac ttcgattagt
    15421 ttgagcgact gcagatcgtc aggcaacttc aagctggctg cgacagcgca cttcgcggtc
    15481 catcgcccca gtcgccagtc agagcggcgc ttggcaaatc gaaggccgct caaccgggca
    15541 gattcgctct cgctaagcca atcgtccgcg gccggaacgt cgttttcgcg ctgctccagc
    15601 caatacacgt ccatcatgaa accagagtgg gagtggccgc atgaatggtg cctgagctct
    15661 cgctttgtag tttggcggag tgcaattccc tctccctggc catgccggat tccaccgcat
    15721 ggatgagata aggacgaagg tccacgggag gaagaatgtg atgcaacgcg cccacttcga
    15781 gggcccgatt cacgctatgc acgcgatcga actcttcggc catttgtccc agcttctcgg
    15841 agtgtacgac tttgaagagc tcgttccatt gagcgcgtaa gcggttcttc tccactccgt
    15901 cggctttggt catcgcctta ttcatggtct cggttaacgc ctgcagacgc ggatctttct
    15961 tggtcctggc ttcgacttca ctggcaaaca ctacagccgc tgccggagcg ccgccgatga
    16021 ctgaggcaaa ggtcccttcc aacgcggcga cttcaagact ctggttcaac gccccagaga
    16081 agacgacata agcgccaccg tgatagcgcg agatcacgca aaacaccatg ggtcctttga
    16141 agttcaccac cgaccgccca atctccgccc cgtactcgag ctgcaacttg cgcaacgatt
    16201 ccggtgaacc atcgaatccc gacaggttag ccaggatcac caccggccgg ttgttgctgg
    16261 ccgcgttgat ggcgcgagca atttttttcg aggagagcgg gaaaagcgtc ccggcggtcc
    16321 attggtccgg accgtcggca ggaacgaatc ctaagcgagg cgccggatgg gattcgatac
    16381 cgatgaggca aaccggatag ccaccgaggt gggcatccca gaccactcct gtttccgcgg
    16441 cacgcatgcc ggaccagcgc tccaaaggaa gttggtcttg gtccatgacg gcccgcatca
    16501 ctttgcggat gtcgaacgac ttcttgcgtc cgggattcgt ctggtcggag aaaatctcac
    16561 ctaccgtggc gaaaccctcg ccgccgtggg tatgcggata gagccggacg tcgcgctcga
    16621 tggggtctgt ggtcacggcc ttgcgaggaa aacgttcgcc cggagccagg taggtgtgat
    16681 cgtagtgacg taacaggatg tggcaggctt catcgatgtt gcgggcccag tattgcgcct
    16741 ggccgttggg acccatgatg cggtcgtagc cgccaatgcc ttcattgtcc tcggctgaaa
    16801 cgcttcccga atactcgaga gcgcgcttgc cggtaagcac catcgccgct ttcggcgtca
    16861 tgacaagaat gccgcgggta tgcatcagca tggtcgcctc agcgttccaa tacggctgcg
    16921 ccccgacgtt gatcccattc accaccaggt tcacttcgca ccctgcttgt gtgaactcga
    16981 tcaggcgacg gaggactttg gcaatccagt ccatgttctc gacgccgctt tccatggaga
    17041 ttttggcgcc ggcggagagg gtgaaccatt ccaacggaat gcctttctct tgtgccagat
    17101 ccagccccgc aaggatgcgc cggcattcgg actcggcgat tgcgcccaga tccttgctcg
    17161 ggtctcccat caaaaccacc cgcgacatgc cttcgggata tttggtggtg aagttgcgga
    17221 ggacgccaac gatgatgttg gacgtgttct tcccatacgg acgattcacc gggaccaagt
    17281 ggccgtcggc atcgagatcg tattccacaa aatcgccggg aggaaactgg gtcgctatcc
    17341 cttccggaac gggcgtcagc attttgacga tttcgtaggg atagatcatt ccgcgctggc
    17401 gcatgcggac aaccttctgg gtgtagtcat ccagcggcgt aagcggttgc tgtttgctag
    17461 cgggcctgat ggtcatgatc tggcccgcgc cgcccgggct cgagatgcgc acgatcatgt
    17521 cgcgcacgcg gccagtatcg ggttgcggaa tgcgggtgcg taccaccacc tgttccaatc
    17581 ccagcccatc ggtggacggg gcgagtttgc gcacgatctg atcgaattct ttgcgggtga
    17641 aattgacggt cggccaaacg taaagcagta tccggttcca atggagccgc ttgttcgggc
    17701 tgcgccgcga ctggaacata cgcataccgg ccaccgcttc cccgaacatg cgttccaggt
    17761 ggggaagttg cgcgatgcgg ccttcctggt cccgcaccgg cgttaggtcg cgaatttccg
    17821 cgaccgcgaa cagccgctcg tccttgggat tgtcattggc aaccgcatag agaaggtaaa
    17881 catcttctac cgacggcagt cgctcgactt tgaaattgtt tagccgccag aggtgcaacc
    17941 gttcggccat catggggtga acgccacgca cgaccaattc ctcttcatat agactcgcag
    18001 agggacgata ggtgaaatgc tgcatcccag ccatgctggg accgcgaccg gggccgccga
    18061 tggccactac gatgcgctgg atcgtccgcg ggaagccgac ttggttaagg gttgagcgga
    18121 cttccttctc gatcagctcc ggatcggaca agaactccgg attccacgca tagaaatcga
    18181 gaacgatttc gtggtcaaca ggcacttcgt cgatgccacg gaacagcgcg cgcgccgcat
    18241 ccgccagccc gagaaattcg gtgttgaggc tgaagatatg aacgcgcttg ccgttatcgt
    18301 catactcggc tgtcacacag gtccggtcgt tcaccggcac ggtgcggaac ttcgacagag
    18361 tctgcccgcg atagtatcgc gtcgtctcaa tctccaacat caattcccgt aaggcaggcg
    18421 gcgcagccgc atacctgccc gaaaagagac cagccagcgg ttgaggacac tcgatcagtg
    18481 cgccaactct ttggcgcagg tccgccgcat ttggattggc cgcgaggtag gcgaggtgct
    18541 cttccgcctg cgcgtagatc tgccgtctgg cccgctcaaa cacgggatgc tcgaagcagc
    18601 gatatcgcac ttcccgggcc agatcgctaa ctgtgggaaa cagcccgcgg gtggtgtgga
    18661 tcaaccgtcc gaggagtttg cgaaaggcct catcggaccg tggcaccgag gcttcgacgc
    18721 gctgcagatg cctttccaac aatccgacaa tcgaagctgt ctgctgctcc acgcggtggt
    18781 gcgatttgca caaccagagc aggcattctt ccagttcggg cgagcgctcc agcgtctgga
    18841 tgccgtagtg aaccatagcg cgccgcagtg catcgacgaa ggctgtcggg aggcctttgc
    18901 cgcgcgtctc aagcatgtgt aaatacgaaa agaggtaggt ctcggcgctc cgttcttccc
    18961 cgccattcag gtcgtccatc tcgggctgcc gctcaaacaa ggtgcatatg tcgacgaaga
    19021 tgccgaggat ttcatcttca ctcttggcta tttcctgata ttcgacggcg acttccgcag
    19081 actggctcca gtcggagagg atcttcatcg catgcgcggg gtcaacatca aaacccagca
    19141 tgagttggcg aagttcttcc aggttcgtgc tccagcgggg ccgaacgggt tcacgagtta
    19201 cgcctgcggt gcgcgatgca ccaaactcca ctcgtccggt tgtggccacg gattcgtcgt
    19261 cgcccgctac cggttcgatt tgcagaagcg gtgccccggt gtccacctgc acatgaggaa
    19321 tcgcagttac gctcctgacc ttgcccgaaa aaggagcgag cactgacatc tccatcttca
    19381 tggcttccag cacggccaag gtatctccga ccttgaccgt gtcgcctggc tttacggcga
    19441 tggaaacgac aaccgccggc gccggcgcgt gtaccactcc accgtcatcc cgggcgaccg
    19501 aatgcgcaac gccattcacc tcgatgcggt aagtcagtcc ttgcacgttg gagacgacgt
    19561 ggaagcggct gccgaacgcg gtgagccaat attcaaactg accgaggctg tacatacgga
    19621 cgtcgatgct ggctccatcc acttccacac ggtagcgatg gaggccgacg caacagacct
    19681 tcagcgagta ggaatgtccc cgatggagta gttgtactgt gcggcccact tcgctgcgaa
    19741 cgtgaggccg cccgcgcacg gccgatgcgt aaaactgtat ccgctccaac ttcaggtcgg
    19801 cggcatacga ttcgatcgct gcctgcacca gcgccacgtc ggcgtagcgc gcggacaggt
    19861 ggccgccctc ggccgaccta cgatccagcc acccaatatc ggcggtcgct ctcaggacct
    19921 cgggacagtt gagcaattcc agcagaaacg ctttgttgct ggctcctccc ttgatgacga
    19981 ctacgctttc gcgcagcgcc cgctgcatcc gtgagagcgc ttccttccga ctctggccgt
    20041 agacaatgat tttggcgatc atcgagtcga attccgccgg cacggcgtcg cccacagcca
    20101 caccggtgtc gacgcgtact cccggtccgg acggaagcct gaatcgctcg atcaggcccg
    20161 gcgccggcgc gaagccgttc tccggatctt cggcgttcag ccgcacctcg atagagtgcc
    20221 cgtacgtaca cggcggctca ccttccaggc gctcccctcg ggcaacatgg atctgcatct
    20281 tcaccaggtc cagcccggtc gtacattccg tgacggggtg ttcgacttgc aggcgggtgt
    20341 tcatctccat gaacaagaac cgctggctga ctggttcgta caagaactcg accgtcccgg
    20401 cgttgcgata gcccgaagcc tggctcagtc gcaccgctgc ctgacgcagc gccaggtcct
    20461 gcgctgcggt caacacgggc gaaggagctt cttccaggac tttctgatgg cggcgctgga
    20521 tggtgcagtc gcgcaccccg acggcccagg tggttccata gtcgtcggca atgacctgca
    20581 cttcaacatg gcgcgcagac tgcaccagtt cttccatgaa aaccgtggcg tccccaaagg
    20641 ccttgaaggc ctcggcgcgg gcgccctcaa atgccctggc aagctggctc gcggaattta
    20701 cccggcgaat cccatgaccg cctcctccag aagtggcttt tatcagcagc ggatagccca
    20761 ggcgctcggc gtgccgcatg gcatcgtcga tggtctccac cggcccgttg ctccaccgcg
    20821 caaccggcac ctcggcttgc tcggccaatc gcttggacga gatcttgtcg cccagacggc
    20881 gcatggcttc actagtcggg ccgacgaata caatgttcat ctcgcggcaa aggtccgcaa
    20941 aatccgggtg ctcggccaca aagccccatc ccacccaggc tgcgtccgca cgcgcgcttt
    21001 ccatcgcggt tcttaaagtt gggaaatcaa cgtaagtgct cttggattga ttggtggttg
    21061 gatcgacgat tcgagcggcg ccgagataca ccgcctcgtc cgcctcgcgc acaaacatgg
    21121 agtggcgatc aggctccgta tagagcgcga tcgtagctat cgatgttcca tgctcccgat
    21181 tgaaatcccg cgccgcttga atgaaacgta tcgcggcttc gccgcggttg acgataacta
    21241 cccgttgaaa ttcgcgctgc atagcccttc ccctggccag agatctgcag ttctacaatc
    21301 ttattcctta cgctatgacg tgttgcaggg cctgcagcgg ctcagcatcg acactgtcgg
    21361 gaagcgttac cgtctggtaa ccggtcaagc gcatgtaccg gttgccctgc ttgtctacta
    21421 cctccgcgtc gaacttctcc cccgccgcgt ctggagtgac aactgcaaac aacgggccat
    21481 cggcaacctc gggagcacgt gaccaactca cttgctcaac aaagagcggc agccccatac
    21541 ggttgtgctc gcttatttct atgaggccgg cggtttggaa acacagctca atcagccgcg
    21601 gcccaatcag cgtcggtccc tgcgatggat cgtgatcgct cggcaaagtc tccgacattt
    21661 ggccgacaat ccgatctcca tcccgccatg cgtgatcgag aacttggtac gccggaccat
    21721 ggaagtaaat gcggtaaatt tgagcggcat cgacgatgcc ctccgacgtc aacttgaggg
    21781 gcacggcggc agacgcttgg tgcggctgct tggtcactcg cacgcgagcg gtgaagtgga
    21841 cggtctcctg gggttgcgat tgattgggaa gcgttcgccg cccgatcagc cggcactctg
    21901 cccgtaaatt gtcgccgtcc gaatagaact gggcctggat aaatacggtg cgtggctcgt
    21961 cgcggtagaa cttgaacggc gccatgaagt ttacttcctc gatggcctcg acactccacc
    22021 ccggatgcat gcagagtgcg gcttcggcaa acgcctcgat ccccatgaca ccgggcagta
    22081 cgggcgtgcc gtcaatgcgg tgatcgtaca agaacggttg ggccttcgga tcgagcgtgg
    22141 tctcaaacgt caaaccactg tagagtccca tgcctgtaac cgtgccgacc atcggccctc
    22201 gcgccggtac tcgctgctcg agagcagcgg ccagcgcttt ggtgtccagt ccaccagtct
    22261 cgtcccattc attcagcatg ataccgagac gctgtgcgac gacaagttcg ccacggttgt
    22321 ctgctgaggt caattcgcgg cgaatcatcg gaacgccagc ttcaggcggc agcatgtcga
    22381 tgccagccag ttccatcatc ttgggtatag agccgcgcgt cgccatccca attccgcccc
    22441 acgctgtcca gtcgatgaca atgccacgcg tacccggccg catggtgcgg aagcttgagg
    22501 tgatcttgca cagcaggtcg ttggcggcgc tgtagtcggt ctgccctcca ttgccgaagc
    22561 gccctgcgat tgagctgaac gccaccgtcg cacccagcgg catgtcgccg atggcatgca
    22621 gaagattgaa ccagccgtca ctcttgacgt cgaagaccag gtcgaattca cgttggtcct
    22681 tctctggcag gaagtgacta cgttcaatgc cggcggcgtg cagcagcaca tcgatacgac
    22741 cgctgcgctg gcgcacttgt ttgatgatat tggtcacacc gtccgcgtcg gtgaggttga
    22801 tgctgaagta gtaaggagtg cctcccgccg aacgcaccgc gtcgatggca ctctgtgccg
    22861 catgtgccct ctccagcccc accagttcct tttccaccaa tgccggtgtg gcgcgctcgc
    22921 cacgcgcttt gatgcgctcg aacagatcgc gcttgagtcc gtctttgtcg ctcacgaagc
    22981 gagccaggtc aggattgttc ggatcgggtt ctggaaccag atccagcagg tagaacgttc
    23041 cacccgatgc ggccgccagg tcggcggtga tggccgacac aatacttccc gccgcgccgg
    23101 tgatcacaaa tacgctgtcc tggtcgagca tcagaccgtg accgccttcg gtaacgggct
    23161 gctcctgcaa ccccactgcc cagcgcaggc cattcttgta gccaacttcg actgcgccgt
    23221 tgtcgcgcaa ggtttcctcg atcatggcga cggcaacttc cgacgcactc cttccagcct
    23281 cgaaatcgac cgccttcacg tgcacttccg gacgctcgcg cttataggtc ttggtgaatc
    23341 cgacaaccgc tccgccgagc ggagcaacgg ccccggcggc gtcatagccg tgttgtccgc
    23401 caagttgcgt ggccgacacc aggaacgaac cgggcgttgc gacctgctcg tacagtgcac
    23461 gcatcgtggt gtacagcgat ttaacgcgca cccgcaaagc ttcgtgccat cccgccagat
    23521 tcatgtgttg gagatcgcct tcgttgtcga gcgcgggcag ccagtacact ccctggatcg
    23581 ggcctgcgga cagccaactc ttgaggcgct ccgtcagggc gtttgcatcg agcgcggcgt
    23641 cgagtaccag agcttcaaca cccagcgtcc gcaggtgctg tgtcagcacc gtgacaacgc
    23701 ctcccttgtc gggcatgacc actactcggc tgccaggccc tagtttcact cccgttggct
    23761 tgcaaatggt caacggcggc cgcaaggtgg gcaccgggac acgacgcgga attaagttgg
    23821 ctgcatcgaa gctggccaag gccggtcgag ggcgtgcaac gtgcttcacc gccgcaggtg
    23881 gcgcgaccgc agcgggagcc gatgccgcgg gcgttacccc taagttttgc ttatcgcgcg
    23941 cgaactggat gacgtgcgcc agtgtaggga aatcgcgcag cttcatgttt tcttcgcgcg
    24001 ggatgttata cgccgcacgc acggacgcga acatctccgc ctgcttgacg gtgtcaaccc
    24061 caaggtcggc ttcgagatcg aggtctagat ccaacatgtc cttgggatag ccggtctttt
    24121 cggcgacaat ttccagcact ttcactctga tggcgtcatc actcaccagc tgtgaggtgg
    24181 cggcaggcgc tacggtcgcg actggttgaa cgattacggg cttcggagaa acgccgggcg
    24241 cgagatcggg acggctctcg tacacgaacc ggatcacgtg ggcgagcgtg ggatagtcgc
    24301 gcagtttgcg gttctcatcg cgcgggatgt tgtagatctc gcggacggcg gcgaacattt
    24361 ccgcctgttt gacagtgtca acgcccaggt cggcttcaag gtccagttcc aagtccagca
    24421 tgtctttcgg atagccggtc ttctccgcca tcaaggtcag aattcgttcc gtgactgact
    24481 cggtttcggc gacaggctga accgctgcga cgggtgcgcg tgcggccacc actgttgcgg
    24541 agaccgttgg cgcagcgggg actggtgcag ttgctactac cggcgcgact ggcgcggtgg
    24601 tagcggctac cttaataggt gccggtgtcg gcgcagttgt ccctgccagg tccgggcggt
    24661 tctcgaacac gaaccgaatt acgtgggcca gtgtcggata gtcgcgcagc ttgcggtttt
    24721 catcgcgcgg gatgttatag atctcgcgga cagccgcgaa catctccgcc tgtttgaccg
    24781 tgtcaacgcc gaggtcggct tccaggtcaa gttccaagtc cagcatgtct ttcggatacc
    24841 cggtcttctc cgccatcaag gtcagaatgc gt
//
LOCUS       DASRKK010000110         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_984128, whole genome shotgun sequence.
ACCESSION   DASRKK010000110 DASRKK010000000
VERSION     DASRKK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984128"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFC15_02490"
     CDS             <1..1158
                     /locus_tag="VFC15_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09059.1"
                     /translation="MSASTPMRFYDADSTPPADTPPLDQPPVVRKRHLNWALKAVLPI
                     AAVLLNGLLLFVLVSLSLAEGERHVIIAVAAAGALLICIAVIMVLAVSVRRPMLELQD
                     KIARVRLGDMDVSVGFASRNDEIGDLGRDFNDMVAQLKASREEIQHLHQTQMSRAEHF
                     ATLGELAAGLAHEIRNPLAGIAGVIEIISRDLPPNSPARSVIKDAKEEAVQINRILTE
                     LLEIARPKPPQFRVGDMCSTVEHAVMFARQQAITKRIMVELEIKDVIPPAEHDPNQIN
                     QVLLNLLLNAIQSMDKPGVIRVSLQSDDDDVLIAVVDEGKGIPPEHLPNIFRPFFTTK
                     GHGTGLGLSLARRMVEAHGGNIDVTSVVGKGTQFRVRLPVKRHTDHGREVS"
     gene            complement(1151..2677)
                     /locus_tag="VFC15_02495"
     CDS             complement(1151..2677)
                     /locus_tag="VFC15_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM09060.1"
                     /translation="MRAAEYARMSTEHQQYSIQNQEATIAAYAEQHGFEIVCSYRDEA
                     KSGLDLAHRPGLRALLDDVANPHRGFTAILVLDVSRWGRFQDTDESAYYEYMCKRAGV
                     RVHYCAEPFANDESLSAVLVKTMKRAMAGEYIRELSAKVFIGQCRIARIGYKLGGSPG
                     YGLRRLLVDRDGRAKSILQEGEWKSLSCDRVTYVPGPPEEVGVVQEIYSRFVNDNWPA
                     SKIAGELNVRGMRRDTGAEWDYFSVYDILKNPKYSGCIVFNRTSTKFRGKTRANPREE
                     WIIQPHSFEALVSPEIFEQAQKRFSTKGRRDKLLEDLHALLQSRKRLTSRIIQGDPHM
                     ASPAAYHRNFGGLKNAYKLIGYERPTNVERVAKDQMRRSRFRMLKLRIMTALLRECVR
                     RSVPVEPYSQGISLIGYGRLEVQIARSLNPAFKGPRWDVVCANPQSRQFMAVRMHVDN
                     IDVYDYLLFRNLPAPRHSRLFIYESEIADFALSFKDIPALIAEIVPIDGGSSGVTNFS
                     "
     gene            2803..3021
                     /locus_tag="VFC15_02500"
     CDS             2803..3021
                     /locus_tag="VFC15_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09061.1"
                     /translation="MSPDPRQLVGKAVRDLRKARNWSQEELAEKAGLHRNYIGGVERG
                     ERNVALLNIVAIAHALKVTPSQLIDRVR"
     gene            complement(3150..>3364)
                     /locus_tag="VFC15_02505"
     CDS             complement(3150..>3364)
                     /locus_tag="VFC15_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZM09062.1"
                     /translation="YLNNILEQDHRAIKRRVNAKQGFREFQAARRTIQGYEAIHMIRK
                     GQARWVAGDDLLRQIQFIDSLFDLAA"
BASE COUNT          763 a          940 c          865 g          796 t
ORIGIN      
        1 atgtcagctt caaccccaat gcgattctac gacgcggatt ccacacctcc ggctgatact
       61 cctcccttgg accaaccgcc ggtggtgcgc aagcgccatc ttaattgggc gctgaaggcg
      121 gtattaccta tcgcggctgt attgctgaat gggttgctgc tgtttgtgct ggtttctctg
      181 tcgctcgccg aaggagagcg gcacgtcatt atcgccgtcg ccgccgctgg tgcgttactc
      241 atttgcatcg cggtcattat ggtgctggcc gtttctgtcc gccgtccgat gttggagttg
      301 caggacaaaa ttgcgcgagt ccggctgggc gacatggatg tctccgtcgg tttcgcctca
      361 cgcaatgacg aaatcggcga cctgggccga gactttaatg atatggtggc acagcttaag
      421 gccagccgcg aagagatcca gcatctccat cagacacaaa tgtcacgcgc tgaacacttc
      481 gccactctag gcgaactagc ggccggactg gcgcacgaaa tccgaaaccc tctggccggc
      541 atcgccggag tgatcgagat cataagccga gacctgccgc cgaacagccc ggcgcgttcc
      601 gtcatcaagg acgccaagga agaggcggta cagattaatc gcattcttac cgaactgctg
      661 gagattgccc ggcccaaacc gccgcaattt cgcgtcggcg atatgtgcag cacggtcgag
      721 cacgcggtca tgttcgcgcg ccagcaggcc atcaccaaac gcatcatggt cgaattggaa
      781 atcaaggatg tgattccgcc ggcagaacat gaccccaatc agatcaacca ggtattgctg
      841 aacctgctgc ttaatgcgat tcaatccatg gacaagcccg gtgtcatccg cgtttccttg
      901 caaagtgacg acgacgacgt gctcatcgct gttgttgacg aaggaaaggg aattccgccg
      961 gaacacctgc ccaacatctt tcgtcccttc ttcaccacca aggggcatgg cactggattg
     1021 ggactctcgc tcgcccggcg catggtggaa gcacacggcg gcaacatcga cgtcaccagc
     1081 gtggtgggca aaggaaccca gttccgggtc cgattgcccg ttaagcgcca cacggaccat
     1141 ggacgcgaag tcagctgaaa ttggtaacgc cagacgatcc tccatcgatc ggaacgatct
     1201 cagcaatgag tgccggtata tctttgaaac ttagtgcaaa gtctgctatc tcggactcgt
     1261 aaatgaagag cctcgagtgg cgtggggcag gtaggttgcg aaacaggaga taatcataca
     1321 cgtcgatatt gtcgacatgc atccgtacgg ccatgaactg cctcgattgc gggttggcac
     1381 atacaacgtc ccaccgagga cccttgaatg ccggattgag gctgcgtgca atctgtacct
     1441 ccaaccgccc gtaacctatc aacgatatac cttgtgaata cggctcgaca ggtacggatc
     1501 tgcgaacaca ttccctcagc aaggctgtca taatgcgcag ttttagcatt cggaagcgcg
     1561 atctgcgcat ctgatctttg gcaacacgtt cgacatttgt tggacgctcg tacccaatca
     1621 gcttatatgc attctttagg ccgccaaagt tgcgatggta ggctgccgga gatgccatgt
     1681 gaggatcgcc ctgaataatc ctactcgtaa gtcgctttcg cgattgaagt aacgcatgca
     1741 gatcctcaag aagcttgtct ctgcgaccct tcgtgctaaa ccgcttctgg gcttgctcaa
     1801 atatctccgg cgaaaccagc gcttcaaaac tatgtggctg tatgatccat tcttctctcg
     1861 ggttcgctcg ggtcttgcct ctaaacttgg tgctcgttcg attgaatact atacagccgg
     1921 agtatttcgg gttctttagg atatcgtaaa ctgagaagta gtcccactct gcgccggtgt
     1981 cacgtcgcat accacgcaca ttgagttcgc ccgcaatctt ggaagcaggc cagttgtcat
     2041 tcacaaaacg agaataaatc tcttgaacca caccaacttc ttctggtggt cctggaacgt
     2101 acgtcactcg atcacaactt aggctcttcc attcaccttc ctgcaaaatg ctttttgcgc
     2161 ggccatcacg atcgacgaga aggcgtctta aaccgtaacc tggactaccg cccagcttgt
     2221 atcctatcct cgctatccga cactggccga tgaatacctt cgctgacagt tctcgaatat
     2281 attctccagc catcgcgcgc ttcattgttt tcaccagaac ggcggaaagg ctttcatcat
     2341 tggcaaatgg ttcagcacaa tagtgcactc gcacgccggc acgtttgcac atgtattcgt
     2401 aatatgcgct ctcatccgtg tcctgaaacc gtccccaacg gctgacatcg agaacgagga
     2461 ttgccgtaaa tccacggtgg ggattggcga cgtcgtccag caatgcacgg aggccgggcc
     2521 gatgcgcaag atccagacca ctcttcgctt catcacggta gctgcacaca atctcgaatc
     2581 cgtgctgctc cgcataggcg gcaatcgtag cctcctgatt ctgaatggaa tactgctgat
     2641 gttccgtcga catccttgcg tactccgcag cccgcatatg gcctccatca acgcaaaatt
     2701 tttatctgtg cagtcgattc actttgcaat agcctgccgc tcatacatgt atcacactca
     2761 tagtgtgtag aacaacaagg cagttacaga cacaattgcg ctgtgtctcc tgatccaagg
     2821 caactcgtag gtaaagcggt tcgtgatttg cgaaaggcca ggaactggtc tcaggaagag
     2881 cttgcggaaa aagctgggct ccacaggaac tacatcggtg gagttgagcg aggcgaacgt
     2941 aatgtcgcgt tgctgaacat cgttgcaatt gcgcacgcgc taaaagtgac gccaagccag
     3001 ctgatcgatc gcgttcgcta gcggtgcggt tgcgatattc cattccggtc tgtgttccgg
     3061 gttggtgttc ccaaagcatg caggctagct cgtagtgttg caagtttcaa atgcagacaa
     3121 gtttgtcgcc gagcgtctgt cgtgcgttct taggctgcca aatcgaaaag gctgtcgata
     3181 aactgaatct gacgaagaag atcatctcct gccacccatc gagcttgccc cttccggatc
     3241 atgtggattg cttcgtagcc ttgaatcgtt cgccgagctg cttggaattc tcggaagccc
     3301 tgcttggcgt tcactcgacg tttgatggct cgatgatcct gttcgagaat gttgttcaag
     3361 tact
//
LOCUS       DASRKK010000111         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1375132, whole genome shotgun sequence.
ACCESSION   DASRKK010000111 DASRKK010000000
VERSION     DASRKK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375132"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFC15_02510"
     CDS             <1..235
                     /locus_tag="VFC15_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09063.1"
                     /translation="PGRMGRLPGFLRLGSTGGLAAGFSNIREALVVKAWCEDRSDSLR
                     YHLAGGRRLIRNKFSGSRNSAGVENGFIGGAKA"
     gene            complement(350..1603)
                     /locus_tag="VFC15_02515"
     CDS             complement(350..1603)
                     /locus_tag="VFC15_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09064.1"
                     /translation="MTRHRFEAYLDKVFGFSALVSALPEGREFPQHAWKKVFDAVFLG
                     TAMQIPSLLQIETECRDGALAKRIGPISDDTIGYALERQSPEPVFALSCEIARRLKRN
                     GVLRSHWSRGLVVAAVDGVEICSSFARCCDACMEREIQHKVNGEMRTDIQYYHRIVVV
                     ALVSTAFPIPLGLRFQKNGEGEVACALALLQDLDEQLGRRFLDVLVGDALYLQSPFVK
                     AVERLGLIWAFTLKENQPELLREAERFTQESPTGVHTEPGREIRYWHLPQVDWPVADC
                     LVRVVKTVRIEHRRRVTVGEKNGHRSKSKTAALQESTNFYATNFELGSISPLFIHQFS
                     RSRWRIDTEVFQTITTDCHLKHPAVHQTTALVVLTMIRFLAYTLSLLFYQRQVRSHAR
                     GKCETFHAFAKRIAYWFVALAADTS"
     gene            complement(1600..1935)
                     /locus_tag="VFC15_02520"
     CDS             complement(1600..1935)
                     /locus_tag="VFC15_02520"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM09065.1"
                     /translation="MKSPREPDLELRGRLIRSLGSLREMLPGSFVERKRACGRPNCHC
                     ADGKRLHSQFQISVLIDGKPKALNIPTELAEKVRQKIEMRRRFDAAAATICGVNLKRF
                     LKEKEERQV"
BASE COUNT          508 a          665 c          741 g          471 t
ORIGIN      
        1 tcccgggaga atgggcaggc tgcctggctt tctgcgtctc ggtagcaccg gcggtctcgc
       61 cgccggcttt tccaacatcc gggaggcgct cgtcgtcaag gcatggtgcg aagaccgcag
      121 cgattcctta aggtatcacc tggctggagg cagacggctg attcggaaca agttcagcgg
      181 ttcacggaac tcggcgggag ttgagaatgg gtttattggg ggtgcaaagg cttgacaggg
      241 gagcctgatc acggggtttt ccaagtcgcg gggaatgccg cggcaccggc gttgctcatc
      301 ggcgcctgag acgatagcag gacacatcgc gaccagcgga ggcgtggaat caactggtgt
      361 cggcggcaag cgcgacgaac caataggcga ttctcttggc gaacgcatga aaggtctcgc
      421 actttccgcg ggcatggctg cggacttggc gctggtagaa gagcagtgac agagtgtagg
      481 ccaaaaagcg gatcatcgtc agcaccacca gggcggtagt ttggtggacc gcggggtgtt
      541 tgagatgaca gtcggtggtg atggtctgaa agacttcggt atcgattcgc caacggctgc
      601 gactgaactg atggatgaac agcggcgaga tcgagccgag ttcgaaattc gtggcgtaga
      661 agttggtgct ttcttgcagc gccgccgtct tgctcttgct acgatgaccg tttttttcgc
      721 cgacggtgac gcgacggcgg tgttcgatgc gaaccgtttt gacgacacgc accaggcaat
      781 cggcgacggg ccaatcgact tgcggcaagt gccagtaccg aatctcccgg cccggctcgg
      841 tgtgaacgcc ggtgggagat tcttgtgtga aacgctcggc ctcgcgcaac agttcgggct
      901 ggttctcttt caaggtgaag gcccagatta gacccagacg ctccactgct ttgacgaagg
      961 gcgattgcag gtacaaagcg tcaccgacca aaacgtccag gaagcgccgg cctaactgct
     1021 cgtcgagatc ttggagcaaa gccagcgcac aggcgacctc tccttcgccg ttcttctgaa
     1081 accgcagacc caaggggatg gggaaagccg tgctgaccaa ggcgaccacg acgatccggt
     1141 gatagtactg aatatcggtg cgcatctcgc cgttcacctt gtgctgaatc tctcgctcca
     1201 tacaggcatc gcaacagcgg gcgaacgaac tgcagatttc gactccatcg accgcagcca
     1261 ccaccaaacc tctggaccag tgggagcgga ggactccgtt gcgttttaag cgacgagcga
     1321 tttcacaact gagggcgaag actggttcgg gagattgtct ttcgagtgcg tagccgatgg
     1381 tgtcgtcgct aatcggtccg atgcgcttcg ccagcgctcc atcgcgacac tcggtctcga
     1441 tctgaagcag gctgggaatc tgcatggcgg tacccaagaa gacggcgtca aacaccttct
     1501 tccaggcgtg ctggggaaac tccctcccct cgggcagagc gctcaccaga gcagagaagc
     1561 caaacacctt gtcgagataa gcctcgaacc ggtgccgcgt caaacctgac gctcttcctt
     1621 ttctttgaga aaccgcttca ggttgacgcc gcagatggta gcggcggcgg catcaaagcg
     1681 tcggcgcatc tcgattttct ggcgcacctt ctcagccaac tcggtaggaa tgttgagagc
     1741 cttcggcttt ccgtcgatca gcacggagat ctgaaattgc gagtgtaggc gctttccgtc
     1801 agcgcaatgg cagttgggcc ggccgcaggc gcgtttgcgt tcaacaaaag agccgggcaa
     1861 catttcgcgg agagaaccga ggctgcgaat gagcctgcca cgcagttcca ggtcgggttc
     1921 gcggggcgac ttcatatgac actaataata gtgtcagaag tggacgaagt caagcactca
     1981 tggagatttg caagaatgcc gcaaaccgaa gtcggaatgg aaaaccctgc aaattaccgg
     2041 gctgacgctg atgccacgcc taccttaagg aatcactgga gggagcgcaa cattacgctc
     2101 ccctgaagac gcggtcgaaa ctactgattc cattgctgcg gcccgccgcg tcttcatcgc
     2161 ttttggtggc ccgccgcggg ccatggggca ctcccttacg gtcgcggctc tgattggagt
     2221 actctcccat gactagcggg gatcaaaaga cgggccgcag ggacgcactg aaaacagtgg
     2281 cggcgggcgg ggcgatgtgg gcggcgcctt cgccccgacc gaacatattg tacctgcact
     2341 cccatgatac aggacgttac ctggaaccct acggctacca ggttc
//
LOCUS       DASRKK010000112         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1277485, whole genome shotgun sequence.
ACCESSION   DASRKK010000112 DASRKK010000000
VERSION     DASRKK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277485"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFC15_02525"
     CDS             <1..1146
                     /locus_tag="VFC15_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM09066.1"
                     /translation="KDFPGFSVGAEEKKLGIRGSSTAPLILNDCMVPVENVLGEIGKG
                     HIIAFNILNVGRFKLGAGCVGGARTSIQNAVAYAKDRKAFGKPISDFGLIREKLAQMA
                     IGIYANESMVYRTVGMMENAMPEGEKSSEVIRKAIEEYAVECSIIKVAGSEMLDFVVD
                     ETVQIYGGYGFVEEYPAERAYRDSRVNRIFEGTNEINRLIITGWLLKRAMSGQLALLP
                     AIKKTMDEVMAGPSFDELEGTLAAEQKIVGNAKKLAMLVAGAASQKYMQKLADQQEIM
                     GAIADMVIESFAMDSCLLRARKFIEANGEQKSALVTAMTQVYIAGAMGRLEANAKKVI
                     AAVAEGDMMRTQIAIVRRLAKHEPVNVIALQELVAARVIETGKYVTV"
     gene            1317..1595
                     /locus_tag="VFC15_02530"
     CDS             1317..1595
                     /locus_tag="VFC15_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09067.1"
                     /translation="MTDLITGLRQVIQDLVAPDLKAILSRQESLQKHQDSLQKQMEVQ
                     HDALVKTIEAFRAEMRSEFAALRANNQLEVLRQVSPLSERLAVVEKKA"
     gene            complement(1624..1911)
                     /locus_tag="VFC15_02535"
     CDS             complement(1624..1911)
                     /locus_tag="VFC15_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09068.1"
                     /translation="MPMSVLDEKRKELEHYEFRMGVARGRLAVALDALTDALTMVGQH
                     GVYCQSARQPGKPAMDIQIVMKSITDAKELITSVMEEIRKQKEAEDSPQRH"
     gene            1990..2526
                     /locus_tag="VFC15_02540"
     CDS             1990..2526
                     /locus_tag="VFC15_02540"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZM09069.1"
                     /translation="MQSRMLFVAVMVAILAAGTTAQEQQKAPLSPAGTATFTFADGKT
                     ITIDYSRPSMRTRKIFGGLVPYGQVWRTGANSATSLKTDVNLTIGGASVPAGSYTLYT
                     IPEEKKWTLIINKQTGQWGTDYDEKQDLARVEMKLSKNASPTEQFTIGFDQTGATSAV
                     LKLDWADTTAKVEVAEKK"
     gene            complement(2858..3667)
                     /locus_tag="VFC15_02545"
     CDS             complement(2858..3667)
                     /locus_tag="VFC15_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZM09070.1"
                     /translation="MDPGRAASLSMDVEAYLHRIKYDGPREPSLATLRGLHRRHMRTV
                     PFENLDIALGNRIQLDPGSVYQKIVMRNRGGYCYELNSLFHHLLKTLRFNVCMLSAQV
                     RREDGSFGPELDHMLLKVTLDEPWIADVGFGDTFVEPLLFVSGASREESGQRFGVANE
                     GGIWEIFKQDKDGKVALYRFADVPRRLSDFEDMNQYQQTSPESGFTRRRICTRATSQG
                     RITLAGMRFIVTSSGLRDERTLRDQSELRRCLRERFGIKFERKTDLSKLTE"
     gene            complement(3640..4764)
                     /gene="holA"
                     /locus_tag="VFC15_02550"
     CDS             complement(3640..4764)
                     /gene="holA"
                     /locus_tag="VFC15_02550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZM09071.1"
                     /translation="MRSFAASDRFVADVQARKLAPGYVFVGDEAFFRKRCRDAVLQQL
                     IPADVRGLSVYDMDLAEVELQQVLDRARTPSLMAPFQVFFIRGVKNLYTRGKHDDEFA
                     AIEDYFKNPNPDALVVFVADHINIPADVRRMDLTDKDRYERIRETLGEYCTVLEFARV
                     DEGDAVKWAIENANAGGVKLDADAARELVDSLGGDMMMVANELEKLVLYVGEKKRITL
                     GDVETMVLAAKQRSFYELTDAISARDRARALAVLDAILNTGDGEEAAIGHLYMLSRTF
                     RQMLVIFERNVRDTRALWQALWQGFRVPPFAAEDVIRQARRYKSRRELTSAIRRIARA
                     DLALRSNPVSKRFVLENLVLDLTAEPKAAEAGWTQEELPV"
     gene            complement(4764..5300)
                     /locus_tag="VFC15_02555"
     CDS             complement(4764..5300)
                     /locus_tag="VFC15_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HZM09072.1"
                     /translation="MKFQPTGSTALVVCVTLLLAAAGCGYHTSGHAVRLPNDIHTLYV
                     PMFDNTTQTFRVEQTFTAAVIQELRSRTSYHIVTTNDGTADATLKGTIYYTANTPLTY
                     DSVTGRISSSVITVNAKVSLVSRAGKTLWDNPNYMYREQYQVSRDVGSFFDESNPAFL
                     RVANEFAKSLVSNILEAY"
     gene            complement(5300..6379)
                     /locus_tag="VFC15_02560"
     CDS             complement(5300..6379)
                     /locus_tag="VFC15_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HZM09073.1"
                     /translation="MKNDPKTPSGAHAEAGSWGGLFRVAIVGAASLKGRELKDVLNDR
                     NFPAHDVRLLDDDESLGQLEAVGDEPTFIQSVLPEHMENVDFTFFASDENFTRNTWSM
                     AKKAGSDIIDLSYGLEGEPDVAIRAPWVEREIGTVPAVSLAGAPVRIAHPAAMVLAVL
                     LLRAQKVSKIIRAVATAFEPASERGKRGMDELHEQTVNLLSFQQLPKSVFDTQVAFNM
                     VDHYGEKSAPSLETIEQRIQRHFKSIVHGKVDVPSLMLLQAPIFHGHAFSLYIEMERQ
                     AKVDELTGALAGEHVDVSQGGEDSPSNVNAAGQEHIQVYVRRDPVRENGFWLWAASDN
                     LRIAALAAVECAESLVAGRPRGTVQ"
     gene            complement(6379..7248)
                     /locus_tag="VFC15_02565"
     CDS             complement(6379..7248)
                     /locus_tag="VFC15_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HZM09074.1"
                     /translation="MSDHPAESPQLVSRSRPKHKGLYILPSLFTAVNIAAGYYAISQA
                     ILGTAGDPWHFDQAAKAIGFAILFDGLDGRIARLTNTTSAFGRELDSLADVIAFGIAP
                     AILAWLWGFRALPVMDDSDLRGKVIQLGAIATFVFLMAGAARLARFNIQSNPQPSNPG
                     RPGKKYFVGMPIPAGAGVIAALVHLVAGNPLQMWWLSIGWAVFVFAIAFLMVSTWRFY
                     SFKELNLGQPRNFRWIIIFGAIIGLGWFFSRYVLFLIALVYTLAGVLARLSFVFRRPT
                     LPPPPPQDEASHA"
     gene            complement(7245..7901)
                     /locus_tag="VFC15_02570"
     CDS             complement(7245..7901)
                     /locus_tag="VFC15_02570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HZM09075.1"
                     /translation="MVRDGYYYGLAFVAVALLLGWFIHPVLAVFPLLLAVFFLWFFRD
                     PERQIPTAEGVIVSPGDGKVTDVSLTEMNGESRTRVSIFLNVFNVHVNRSPIAGEITA
                     VNYKRGKFGNAMGAVSAEENEQNVVTVRGDSGTVIFKQIAGLLARRIVFNKKVGDHVA
                     RGERVGLIKFGSRVDVIFPAGAEIKVKVGDHVAGGSSVLAVCQACVVEFGSRKAAEAR
                     "
     gene            complement(8098..8325)
                     /locus_tag="VFC15_02575"
     CDS             complement(8098..8325)
                     /locus_tag="VFC15_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZM09076.1"
                     /translation="MATQVRDQLLASNDNFRKLLEEHSQYSQRLEALTTKKYLSEDEK
                     LEEVRLKKLKLRLKDQMESLEQQFRNDHQVV"
BASE COUNT         1826 a         2468 c         2475 g         1657 t
ORIGIN      
        1 aaagacttcc ccggcttctc ggtcggcgcg gaggagaaga aactcggcat tcgtggctcg
       61 tccacggccc cgctcatcct caacgactgc atggtgccgg tggagaacgt cctcggcgag
      121 atcggcaagg gacacatcat cgcattcaac attctgaacg tgggccgctt caagctgggc
      181 gcgggctgcg tcggcggagc gcgcacctcg attcagaacg ccgtcgccta cgcgaaggac
      241 cgcaaggctt tcggcaagcc gatttccgac ttcggcctga tccgcgaaaa actggcgcag
      301 atggcgatcg gcatttatgc caatgaatcg atggtctatc gcaccgtcgg catgatggaa
      361 aacgccatgc ccgaaggcga gaagagcagc gaggtcattc gcaaggcgat cgaggagtac
      421 gcggttgagt gctccatcat caaggttgcg ggctcggaaa tgctcgactt cgtggtcgac
      481 gaaaccgtgc agatttacgg cggctacggc ttcgtcgaag agtacccagc ggagcgggcg
      541 tatcgcgact cgcgcgtcaa ccgcatcttc gagggcacca acgaaatcaa ccgcctcatc
      601 atcacgggat ggttgctgaa gcgtgccatg agcggacagc tcgcgctgct gccggccatc
      661 aagaagacca tggacgaagt gatggcgggc ccatcattcg atgaactcga gggcaccctg
      721 gctgccgaac agaagattgt cggcaacgcc aagaagctcg cgatgctggt ggccggagcg
      781 gcttcgcaaa agtacatgca gaagctggcc gatcagcagg aaatcatggg cgcgattgcc
      841 gacatggtga tcgagtcatt tgccatggat tcctgcctgc tgcgggcgcg caagttcatc
      901 gaggccaatg gcgagcagaa gagcgctctg gtgaccgcga tgacccaggt ctacatcgcg
      961 ggtgcgatgg gccgtctgga agccaatgcc aagaaagtga tcgcggccgt ggccgaaggc
     1021 gacatgatgc gcacccagat tgcgattgtc cggaggctgg cgaagcacga accggtcaac
     1081 gtgatcgcgc tacaggaact ggttgcggct cgggtgatcg agaccggcaa gtacgtaacc
     1141 gtttagagga gttctaacca cggaggcaca gagagaccgg gaagagttgg aaacggtttc
     1201 tctgtgcctc cgtgcctcag tgcctctgtg gtgaacgatg tcgcttcgct ccccgctttg
     1261 cgttgcgact cgaccgttta cttgcggcac cgggcaaccg gttgtaggat tgcgccatga
     1321 cagacttaat caccggattg cggcaggtga ttcaggactt ggtcgctcct gatctgaaag
     1381 ccattctgtc ccgtcaggag tcgcttcaaa aacaccagga ttccctccag aagcagatgg
     1441 aggtccagca cgacgcgctg gtcaagacca ttgaagcctt cagggctgaa atgcgttctg
     1501 agtttgcagc gttgcgggcg aataaccagc tcgaagttct gcgtcaagtt tctcccttga
     1561 gcgagcggct ggcagtagtc gagaagaaag cctagatcca aagtcgaatg tagataagtc
     1621 ttctcagtgc ctctgtggtg aatcttccgc ttctttctgt ttgcgaatct cttccatcac
     1681 cgacgtaatc aattccttgg catccgtgat gctcttcatc acgatctgga tatccatagc
     1741 aggcttgccg ggttgacgag cgctctggca atagacccca tgctgaccca ccatggtgag
     1801 tgcgtcggtc aaggcgtcga gagctaccgc gagccttcct cgcgccacac ccatacggaa
     1861 ctcgtaatgc tcaagctctt tgcgcttctc atccagtacg ctcatgggca tgattgtaac
     1921 gtctgccgcg aacgtgaata ataggatggc cgccatcgtc gtaagattgg aacgtcaaag
     1981 gagagttcaa tgcagagccg catgttattc gtcgccgtca tggttgcaat tctcgccgca
     2041 ggcaccactg cccaggaaca acagaaggcg ccactgagcc ctgcgggaac cgcgacgttc
     2101 accttcgctg atgggaagac catcaccatc gattacagcc gcccctcgat gcgcacccgg
     2161 aagatctttg gcggactggt tccctacgga caggtctggc gtacgggcgc gaactccgcc
     2221 accagcctca agaccgacgt gaacctaacc atcggcggcg cgagcgtccc cgcaggcagc
     2281 tataccctct atacgatccc ggaagagaag aaatggacgc ttatcattaa caagcagacc
     2341 ggacagtggg gaacggatta cgacgagaag caggacctcg cgcgcgtgga gatgaagctc
     2401 tccaagaatg cctcgcccac cgagcaattc accatcggct tcgatcagac cggcgcaact
     2461 tccgcagtcc tgaagctcga ctgggccgac acgaccgcca aggtggaagt cgccgagaag
     2521 aagtagacct acgtttcaga ggtgtcgctg tgctggtgct ggtctctcag gacccgcgcg
     2581 atttcagcgc aatcaaactt atggacaaaa ataccaagat cgagattcgt cgttgcctgg
     2641 tttgaaccgg tttacgacgc ttggccggga gcctgaagcg cgaactcaaa tcgaagcacg
     2701 gcattcggcc gagaatgcgg cacgcctccc atgaactcga agttttggcg gatggccgta
     2761 gcgtcttttc ctatcagcgc gcgcaccgga ttccgacggt cgagagtctc ctgaccctga
     2821 tcgaagccgg aggctaaaca ataccgtccg cagaaactca ttctgtgagt tttgacagat
     2881 ccgtcttccg ctcgaactta atcccaaatc gttcgcggag gcaccgacgt agctcagact
     2941 gatcacgcag cgtgcgctca tcgcgcaggc cgctactggt aacgatgaat cgcattcccg
     3001 ccagcgtaat tcgtccctgc gacgtggccc gggtacagat gcgacgtcgc gtgaaccccg
     3061 actccggaga ggtttgctgg tactgattca tgtcctcaaa atcggaaagg cggcgtggca
     3121 cgtcggcaaa tcgatagagc gcgaccttac cgtccttgtc ctgcttgaat atctcccaaa
     3181 ttccaccctc gttcgccacg ccaaatcgct gcccgctctc ttcacggctg gctccggaca
     3241 cgaatagcag cggttcgacg aacgtatccc caaagcccac atcggcgatc catggctcat
     3301 cgagcgtgac cttgagcaac atgtgatcca gttccggccc aaaacttcca tcttcgcgtc
     3361 gcacctgcgc cgacagcatg cagacgttaa acctcagggt tttgagcagg tgatgaaaca
     3421 ggctgttcag ttcgtagcaa taccctccgc ggttgcgcat cacaatcttc tgatataccg
     3481 agccggggtc gagttggatg cggttgccga gcgcgatgtc gaggttttcg aacgggacag
     3541 tgcgcatgtg ccggcgatgc aggccgcgaa gcgtcgcgag tgacggctcg cggggaccgt
     3601 cgtatttaat gcgatggaga tacgcctcga cgtccatgct tagactggca gctcttcctg
     3661 ggtccatcca gcttcggcgg ctttcggctc cgcggtcaga tcgagcacca ggttctccag
     3721 cacaaaccgc ttgcttaccg gattcgatcg aagcgcaagg tcagcccgcg caatgcgccg
     3781 aatcgcgcta gtgagttcgc gccgtgactt gtaacgccgg gcctggcgaa tgacgtcttc
     3841 ggccgcgaac ggtggcacgc gaaagccctg ccagagcgcc tgccacagag ctcgcgtgtc
     3901 gcggacgttg cgctcgaaaa tcaccagcat ctgccggaaa gttcgcgaga gcatgtagag
     3961 atgtccgatg gcggcttcct cgccatcgcc ggtgttcagg attgcgtcca ggactgcgag
     4021 agcgcgggcg cgatcgcggg ctgagatggc gtcggtcagt tcgtagaacg agcgttgctt
     4081 tgccgccagc accatagtct cgacatcgcc gagcgtgatg cgcttctttt cgcccacgta
     4141 gagcaccagc ttctcgagtt cgttggctac catcatcatg tcgccgccaa gcgaatccac
     4201 cagttcacgt gcggcgtcgg catcgagctt cactccccca gcgttggcgt tctcgatggc
     4261 ccacttcacc gcgtcgccct cgtccacgcg cgcgaattcc agcacggtgc agtactcgcc
     4321 gagtgtctcg cgaatacgct cgtagcggtc tttgtcggtc aggtccatgc gacggacgtc
     4381 ggcgggaata ttgatgtggt cggccacaaa caccaccaag gcgtcgggat tgggattctt
     4441 gaagtagtcc tcgatggcgg cgaactcgtc gtcgtgcttg ccgcgagtat agagattctt
     4501 gactccacga atgaaaaaga cctggaaggg cgccatcagc gacggcgtac gtgcccggtc
     4561 cagaacctgc tgcaactcga cttccgcgag atccatgtcg tacacgctca acccgcgtac
     4621 atcggcggga ataagctgtt gcaggacggc atcgcggcaa cgtttgcgga agaacgcctc
     4681 gtcgcctacg aagacgtagc ctggcgccag cttgcgggcc tgcacatcgg ccacgaaccg
     4741 gtctgacgcg gcgaagctgc gcactaatag gcctccagga tgttgcttac cagcgacttg
     4801 gcgaactcat tagcgacacg caggaacgcg ggattcgact cgtcgaagaa gctgccgacg
     4861 tcgcgagaca cctgatactg ttcgcggtac atgtaattcg gattgtccca cagcgtttta
     4921 cccgcccggc tgaccagcga gacctttgcg ttgacggtta tcaccgacga ggaaatgcgt
     4981 ccggtgaccg aatcgtaggt caaaggagta ttcgcggtgt aatagatcgt cccctttagg
     5041 gtggcatcgg ccgtaccgtc gttggtagtt acgatgtggt agctggttcg gctgcgcaat
     5101 tcctggatca cggccgcggt aaaggtttgc tccacgcgaa acgtctgggt ggtgttgtcg
     5161 aacatgggca cgtagagcgt gtggatgtcg ttcggcagac gcaccgcatg gccgctggtg
     5221 tggtatccgc atccggcggc ggcaagcaac agggtgacgc aaaccacaag agccgtcgaa
     5281 ccagtgggtt gaaacttcat cactgcacag ttccccgcgg acgaccggcg accaatgact
     5341 cggcgcactc caccgctgcc agcgcggcaa tgcgcaagtt gtcggaagcc gcccacagcc
     5401 agaatccgtt ttcgcgcacc gggtcgcggc gtacatacac ctgaatatgc tcctgtccgg
     5461 cggcgtttac gttgctgggc gagtcctcgc cgccctgtga cacgtccacg tgctcgccgg
     5521 cgagcgcgcc agtcaactca tccaccttgg cctgacgctc catttcaatg taaagcgaga
     5581 aggcatggcc gtgaaagatc ggcgcctgca gcagcatcag cgaaggtacg tccactttgc
     5641 cgtggacaat gctcttgaaa tggcgctgaa tacgctgctc aatggtttcc agcgacggcg
     5701 cggatttctc gccgtagtga tcgaccatgt tgaacgcaac ctgggtgtcg aaaacactct
     5761 tgggcaactg ctggaacgag agcaggttca ccgtctgctc gtgcagttcg tccatgccgc
     5821 gtttgccgcg ctcggaagca ggttcgaaag ccgtggcaac cgcccgaatt attttcgaga
     5881 ccttctgggc gcgcagcagc agtacggcga gcaccatcgc cgccggatgg gcgattctta
     5941 ccggggcgcc ggcgagacta acggcaggca cggtgccgat ttcgcgctcg acccaggggg
     6001 cgcgtatcgc cacatcgggc tcaccttcta acccatacga gagatcgatg atgtcgctgc
     6061 cggctttttt cgccatgctc caggtattgc gggtgaaatt ctcatcggaa gcgaaaaagg
     6121 tgaaatcaac gttctccatg tgctcgggca ggacactttg aataaaggtc ggctcatcgc
     6181 ccaccgcttc tagttggccc agcgactcgt cgtcgtcgag taggcgaacg tcgtgggccg
     6241 gaaaattgcg atcgttgagc acatctttca gctcgcggcc cttgaggctg gcagctccga
     6301 cgatcgcaac gcggaacagt ccgccccagc ttccggcctc ggcatgtgct ccagaggggg
     6361 tcttgggatc attcttcatc aggcgtgaga ggcctcgtcc tgcgggggag ggggcggcaa
     6421 ggtgggccgc cggaacacaa aggagagccg cgccagcaca cctgcgaggg tgtacaccag
     6481 cgcgatcagg aacagcacat atcgcgagaa gaaccagccg agtccgatga ttgcgccaaa
     6541 aataatgatc cagcggaagt ttcgaggctg gccaagattc aactctttga agctataaaa
     6601 ccgccaggta ctgaccatca ggaaagcgat ggcaaagacg aaaaccgccc agccaatcga
     6661 aagccaccac atttgcagcg gattgccggc caccaggtgg accagcgccg cgatcacgcc
     6721 agcgcccgcc ggaatcggca tgcccacgaa atattttttg ccggggcgtc cgggattgga
     6781 aggctgcggg ttggactgaa tgttgaaacg cgccaggcgc gccgctcccg ccatgaggaa
     6841 tacgaaagtc gcaatcgcgc ccagttgtat gacttttccg cgaaggtcgg aatcgtccat
     6901 gaccggcagc gcgcgaaagc cccagagcca cgccagaatc gcgggcgcaa ttccgaaggc
     6961 gatgacatcg gccagcgagt caagctcgcg gccaaacgcg ctggttgtgt tggtcagccg
     7021 ggcgatgcgg ccgtcgagtc catcgaacag gatggcaaac ccaatggcct tggccgcctg
     7081 atcgaagtgc caggggtcgc cggccgtgcc tagaatggcc tgcgaaatag cgtaatagcc
     7141 ggcggcaatg ttgaccgccg taaacaagga aggcagaatg tagagcccct tgtgtttagg
     7201 gcgcgaccgc gagactaact gcggggattc ggccggatga tcgctcatct cgcctccgcc
     7261 gccttacgcg agccgaactc cacgacgcat gcctggcaaa ccgccagcac ggaagaacct
     7321 ccggccacat gatctccgac tttgactttg atttcggcgc cggccggaaa gatcacgtca
     7381 acccgcgagc caaacttgat gagtcccacg cgctcgcctc gggccacatg atcgccaact
     7441 ttcttgttga acacgatgcg gcgcgccagt agtccggcaa tctgcttaaa aatgacggta
     7501 cccgaatcgc cgcgcacggt taccacgttc tgttcgttct cttcggctga aaccgccccc
     7561 atggcgttgc cgaatttgcc gcgcttgtag ttcacggcgg tgatttcacc cgcgatcggc
     7621 gaacggttca cgtgaacatt gaagacattt agaaaaatgc tgacgcgagt gcgcgactcg
     7681 ccattcatct ccgtaaggga gacgtcggta accttgccat ctccgggaga cacgatgacg
     7741 ccctcggcag tcggtatctg gcgctcggga tcgcggaaga accacagaaa gaacaccgcc
     7801 agcagcagcg gaaacaccgc cagcacaggg tgaataaacc agcccagcag cagggcaact
     7861 gccacaaaag ccaacccgta atagtagccg tcacgcacca tgagtgtccc gatgattata
     7921 gaaccttggg tgcgaggatg cgggaagtgg gcgacgcaga attctaatga gtcggttcaa
     7981 ttgcaggaat tttgcctgga ctttacgcaa gatcactagc ggcgtgggga cgatacaact
     8041 tcggcgggac gataaagatg ccggccctta tcgccgtgga gacaacctaa tgctcagcta
     8101 aacgacctgg tggtcgttcc ggaactgctg ttccagcgat tccatctggt ctttcaggcg
     8161 gagtttcagc ttcttcagac gcacttcctc aagcttttcg tcctcactga gatacttctt
     8221 ggtggtgagg gcttcgagcc gctgagaata ctgcgaatgt tcttccaaca gtttgcgaaa
     8281 attatcgttg ctggcgagaa gctggtcgcg aacctgagtt gccattcagt gaacctccgg
     8341 gaattacgac agaccaaaac gctagcatac agccttttcg gcttcaaggg attttgtcac
     8401 aaatctgcaa tcttgaggcg gtagag
//
LOCUS       DASRKK010000113         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_691258, whole genome shotgun sequence.
ACCESSION   DASRKK010000113 DASRKK010000000
VERSION     DASRKK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691258"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="pyrH"
                     /locus_tag="VFC15_02580"
     CDS             complement(<1..297)
                     /gene="pyrH"
                     /locus_tag="VFC15_02580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZM09077.1"
                     /translation="MSTPIYKRILLKISGEALAAGQGFGVDNTRVHEIAAEIGEVHRL
                     GIEIAIVVGGGNFFRGVAEQAKLMDRVSADHMGMLATVINALALQDALEKQNVFT"
     gene            537..2090
                     /locus_tag="VFC15_02585"
     CDS             537..2090
                     /locus_tag="VFC15_02585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HZM09078.1"
                     /translation="MDNVDDLLRQVFQLAEFRPHQREIIQDVLNGNDVVCVMPTGAGK
                     SLCFQLPALALKGLTVVVSPLISLMADQVRHLQTLGVPVLLLNSSLPSSEQSQVLKRL
                     NDGFAGLLYLAPERFAAPSFHRLLPQLRPNLLVVDEAHCLSFWGHDFRPEYMRLAEVR
                     EQLGSPVTMALTATATPNVRDDIIKMLRLRLPKVHVTGFDRPNLTYACRRLDTEREKD
                     EALLHGLSKQPGSGIIYCSTRRLVEQLTALFEERFPRRTVRAYHAGMDAQARKKSQED
                     FMANLDALVVATNAFGMGINKADIRFVVHYNIPASLEAYYQEAGRAGRDGRPAQCILY
                     CSPRDRFTQEFFIDKIGENNTVLSPSQVETLQTHARQKLDQVFNYAYRSRCRRRAILD
                     YFGDHAAITRCTCDVCRGDVRSRPPAPLTGGIAPRRLAKAKPASSDEDHLDQAAELRL
                     ERLKKVRSELAAEQKWPAFCIAHDSVLREVARAAPTSLDALADIRGIGANKLQKFGAA
                     FLQAIKGSS"
     gene            complement(2124..2348)
                     /locus_tag="VFC15_02590"
     CDS             complement(2124..2348)
                     /locus_tag="VFC15_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09079.1"
                     /translation="MEVHLNPEQASKLAQLASDRGRDADALAQEAITRYLEEEARFLE
                     AVKLGESQLERGEYLTHEEVGSRIERLLES"
BASE COUNT          528 a          743 c          714 g          538 t
ORIGIN      
        1 ggtgaaaacg ttctgcttct ccagcgcatc ctgcagcgcg agggcattga tgacggtggc
       61 caacatgccc atgtgatcgg cggagacgcg gtccatgagt ttggcttgct cggccacgcc
      121 gcggaagaag ttgccgccgc cgaccacgat cgcgatctcg attcccaggc gatgtacctc
      181 gccgatttcg gcggcaatct cgtggacgcg ggtattgtcc acaccgaagc cctggccggc
      241 ggccagcgct tcaccggaaa ttttcaggag tatgcgttta taaatcggag tgctcatttt
      301 tgggcaaaga tacctagctg gaaaagtgct gattcagttt agcaggaaga atcgacagcc
      361 gataacccct agcccgcagg gcgaaacaaa tgtggcccag gacgtgagtc ctagggaggc
      421 gttcacaaaa taattcgagt cccgcaggga cggccgaggt tcccacccgg actctctgcc
      481 ctccgctcag cctctcatga cctgagacag gttaaagtag ttgagggctt tgggtcatgg
      541 acaatgttga cgatctcctg cgacaagttt ttcagctcgc ggagtttcgc ccccatcagc
      601 gggagattat ccaggatgtc ttgaacggca atgatgtggt ttgcgtcatg ccgacgggcg
      661 cgggcaaaag cctttgcttt caacttccgg cactggcgct gaaaggcctc accgtcgtag
      721 tctctccctt aatctcactc atggcagacc aagtacgtca cttgcagacc cttggtgttc
      781 ccgtacttct gctgaacagc tcgctgccat cgagcgagca atcgcaggtc ctcaagcgac
      841 tcaatgacgg cttcgctgga ttgctctacc tcgcccccga gcgcttcgcc gctccttcct
      901 ttcatcggct cttgccccaa ctgcgaccca acctgctggt ggtggacgag gcccactgcc
      961 tcagcttttg gggacacgac ttccgccctg agtatatgcg cctggccgag gtgcgcgagc
     1021 agttgggatc gccggttacc atggctttga ccgcgaccgc cactcccaac gtccgtgacg
     1081 acattatcaa aatgcttcgg ttacgcctcc caaaggtaca cgtcacaggc tttgatcgcc
     1141 ccaatctcac ctatgcctgc cgccgcctgg atacggaacg ggaaaaggat gaggccctgc
     1201 tgcatggtct ttccaagcag ccgggcagcg gcatcatcta ctgttctact cgccggctgg
     1261 tagagcagct aaccgcgttg ttcgaagagc gctttccccg ccgcacggtg cgcgcctatc
     1321 acgctggcat ggatgcccag gcccgcaaaa agagccagga agacttcatg gctaacttag
     1381 atgcgctggt ggtggcgacc aacgcctttg gcatgggaat caacaaggct gacatccgct
     1441 ttgtggtcca ttacaacatt cccgccagcc tggaagctta ctaccaggag gctggccgcg
     1501 ctggacgcga cggtcggccc gcgcagtgca ttttgtattg cagtccgcgt gatcgattca
     1561 cgcaggaatt cttcatcgac aagatcgggg agaacaacac cgtgctttcc ccgtcgcaag
     1621 ttgaaacgtt gcaaactcac gctcggcaga agcttgacca ggtattcaac tatgcctatc
     1681 gatcgcgatg ccgccgacgt gccattctgg attacttcgg cgaccatgct gccattaccc
     1741 gttgcacatg tgatgtttgc cggggagatg tacgctcccg cccgccagcg ccattaacag
     1801 gaggtattgc cccgcggcga ttggcgaagg caaaacccgc cagttcggac gaagatcatc
     1861 tcgatcaggc tgcggaacta cgactcgaac gcttgaagaa ggtaaggagc gagttggcgg
     1921 cggagcagaa gtggccagcg ttctgcattg ctcacgactc agttttgcgc gaagtggcgc
     1981 gtgccgcacc gacatcgctg gatgcgctgg cagatatcag aggtataggc gcgaacaaac
     2041 tgcagaaatt cggggccgca tttctgcagg cgattaaagg atcgtcgtag cgggatccgt
     2101 agagatggat ctgccgacca ccatcaggac tcgaggaggc gctcgatgcg agatcccact
     2161 tcttcatgtg tgaggtattc gccccgctcc aattgagatt cgccgagctt cacggcttcg
     2221 agaaagcggg cctcttcctc cagatagcgg gtaatcgctt cctgagctag agcatccgcg
     2281 tcccgtcctc gatcgctggc gagttgcgcc agtttggagg cctgctcggg gttgagatgt
     2341 acttccatgg gctgaggtta ggagtgacag cagcgcttgt caagttacag attggcgcct
     2401 gtactaagac acactccaca ccgtttgaag tggcaaaaat caacagggcg ggaacatgtc
     2461 ccgccctgct aaatataccg ccagaatcgc aacaacttac tcggctgcct ggtccgcggc
     2521 ctt
//
LOCUS       DASRKK010000114         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1179807, whole genome shotgun sequence.
ACCESSION   DASRKK010000114 DASRKK010000000
VERSION     DASRKK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179807"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..670)
                     /locus_tag="VFC15_02595"
     CDS             complement(269..670)
                     /locus_tag="VFC15_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09080.1"
                     /translation="MPSVDEIEEDPSFIGDVLAYMNSPEGELFIEVGDTVSDVLERAD
                     VDAKERQIFWEDGERLSITASARRIGVEHPDYPLDIIEQNVIIWLESEFAPPTYTPEQ
                     LDELDQLTEAWIQDHKRQAKRDQKGSRTRHS"
     gene            complement(724..1041)
                     /locus_tag="VFC15_02600"
     CDS             complement(724..1041)
                     /locus_tag="VFC15_02600"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM09081.1"
                     /translation="MAKTRIEQISNRIERIKTELAAIDEMRPGSVSKQFKEPDVERGA
                     YYQLNYSQGGRSRTEYVAPAFVPDVRRQISNYKRFKALTTEWVELSIEQSRLKIKSAT
                     SKP"
     gene            1109..2509
                     /locus_tag="VFC15_02605"
     CDS             1109..2509
                     /locus_tag="VFC15_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09082.1"
                     /translation="MPLWVLWWNALQMLRPAFSRLQTFLWFATTVAGFTVRSEHLGVT
                     SIVRALKLKPGCYGYVIKHMHSSAVRINDLNALWTRVVLKLFPSPIRVNGRRVLVGDG
                     IKAPKRGKKMPGVKLLHQQSESNNKSEYIMGHSIQAVSVLIKAANSVLAVPLAMRIHE
                     GLIWSNRDRRTLLDKMLSLINTLALSEPFYFVGDAYYAAGKMADGLLEHGNHLITRVK
                     SNAVAYESYVAHGPKTRGRPRLYGQKIALKALLNDTTGVQQAPSPVYGENDVILSYHV
                     RDLMWRPTGRLARFVAVTHPSRGSCLLMSTDITLSAVDIVRLYGLRFKIEHTFKQAVR
                     SIGTFAYHFWMKDMKPLRRGSGNQHLHHESKKYRDGVKRKTHAYHVFMQAGVVSQGLL
                     QYLAAVAPTQVWNSFGSWLRTVRPGVAPSEFVVAGALRHTLPEFLLNNREQASIAKFI
                     VENQNLDTMEMFGVAA"
BASE COUNT          601 a          757 c          674 g          604 t
ORIGIN      
        1 tcttacctta gaccggcgac gaggtccatc caggcagcga ccgcgcggcg aaaaatcctg
       61 ctactttaac cgcacggaaa accgccaaga catcgagcct cgttctgcag ttgtacggtt
      121 tcaaagtacc cccgacttaa acttgcggct aggatagtgt aggatcaccg cttgcgtatt
      181 agtatcgtca aaatggcacg ggaatcaact tgcaatgagc aaacgcacga agccgcgatt
      241 catcgtcgca acatcatcaa atcatcactc aagagtggcg agttcttgac cctttctggt
      301 ctctctttgc ttgccgcttg tggtcctgaa tccaggcctc ggtgagttga tcgagctcgt
      361 cgagctgctc cggcgtgtag gttggcggag cgaactccga ctcaagccaa atgatgacgt
      421 tctgctcgat gatgtcgagc ggatagtcag gatgctccac tccaatgcgc ctcgctgatg
      481 ccgtaatcga tagccgttca ccgtcctccc agaagatctg acgctctttg gcgtcgacat
      541 ctgctcgttc aagaacgtcc gatacggtgt cgcccacttc aatgaagagt tcgccttccg
      601 gcgagttcat ataagccaga acatcgccga tgaacgacgg atcttcctca atttcatcaa
      661 ctgatggcaa tcggtattcg atgctccagc ccttgctcac gcgcaaagta taccatagcg
      721 ttctcagggt ttgctagtag ctgatttgat cttgaggcga ctctgctcga tgctcagctc
      781 gacccattcc gtagtcagcg ctttgaaccg cttgtagttg ctgatttggc ggcgcacgtc
      841 gggtacaaac gcaggcgcta catattcagt tcgacttctc ccgccctgtg agtagttgag
      901 ctggtagtac gcgccgcgct cgacatcggg ttctttgaat tgcttgctca cggatcccgg
      961 tcgcatctca tcgatcgcgg ccaactccgt cttgatccgc tcgatgcggt tggagatttg
     1021 ctcgattcgt gtctttgcca tatttacagt atagcttaca taagctatac tgtaaataat
     1081 tacacggcaa ctacagatcg gggttccgat gccgttgtgg gtcttgtggt ggaacgcgct
     1141 gcaaatgctg cggccggcgt tctcccgatt acagactttc ctttggtttg cgacgacagt
     1201 ggccggattt acggtgcgta gcgaacactt gggcgttacg agcattgttc gggcgctcaa
     1261 actcaaaccc ggatgctatg gctacgtgat caagcacatg cacagttcgg ccgtgcgaat
     1321 caatgacctc aatgcattgt ggacacgtgt tgtcttgaag ctattcccga gccccatacg
     1381 cgtcaacggc cgacgcgttc tcgtcggaga cggcatcaag gcgccaaagc gcggaaagaa
     1441 gatgcccggt gtcaagctgc ttcaccagca gtctgagtct aacaataaat ccgaatacat
     1501 catgggccat tccattcaag ccgtgagcgt gctcatcaag gcggccaaca gcgtcttggc
     1561 cgtgccgttg gcaatgcgaa ttcacgaagg gctcatctgg agcaaccgcg accgccggac
     1621 gctcctggac aaaatgctct cgcttatcaa caccctcgca ctctccgaac cgttctactt
     1681 tgtcggcgat gcatactacg ccgcgggaaa gatggccgac ggattacttg agcacggcaa
     1741 tcacctgatc acgcgagtga agtccaacgc tgtcgcctac gagtcttacg tcgcccatgg
     1801 accaaaaaca cgtggccgac ctcggctcta tgggcagaag atcgcactca aagcattgct
     1861 caacgacacc accggagttc aacaggcgcc gagtcccgtt tacggcgaga acgacgtcat
     1921 actcagctac cacgttcgtg acctcatgtg gcgcccgaca ggacgactcg caagatttgt
     1981 cgcggtcact catccgtcac gaggatcgtg cttgctcatg tcaaccgaca ttacccttag
     2041 cgcggtcgac atcgtccgac tctacgggct ccgcttcaag atcgagcata ccttcaaaca
     2101 agcggtgcgt tcgatcggca cgttcgcgta tcacttctgg atgaaagaca tgaagccgct
     2161 gcgccgcggc agcggcaacc agcatcttca tcatgagtcc aaaaagtatc gtgacggcgt
     2221 caaacgtaag acgcatgctt atcacgtatt catgcaggcc ggtgtcgtat cgcagggact
     2281 acttcagtat ttagcagccg tcgctcccac ccaggtttgg aactctttcg gctcctggct
     2341 gagaaccgtc cgacccggag ttgcaccatc ggagttcgtc gttgccggtg ccctacgaca
     2401 cacgctgccg gaatttctcc tgaataaccg cgaacaggct tccatcgcga aattcatcgt
     2461 tgagaatcaa aaccttgaca ccatggagat gttcggcgtc gcagcctgac agaacttgcc
     2521 actcatgagt aaggaaaaca aggtaaagta aggaggagga atcgcttcga ccacattgac
     2581 ctcgaaaggt cagatgacgc ttcccaaaga gattcgcgat cgcctgaacc tgaagc
//
LOCUS       DASRKK010000115         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1180020, whole genome shotgun sequence.
ACCESSION   DASRKK010000115 DASRKK010000000
VERSION     DASRKK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180020"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFC15_02610"
     CDS             <1..597
                     /locus_tag="VFC15_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM09083.1"
                     /translation="WTYENGPINGNVDAWTINFGFTVSDSYLAAGTSVNGFEFGVRVV
                     PGNVVNGVDWILSTGPCSDPGSGCGTIVGFGTATSNLTDQFLFINNFGYEIDLITVSN
                     LNVPEIGGQGYYLTLANATTVQSLEPVYWDENSGVGCHSAGCPSTAYENTLGTIPSEA
                     FTIGGGSGGGTTPEPSSILLFGSGIVGLAGVLRRKLNR"
     gene            958..>2262
                     /locus_tag="VFC15_02615"
     CDS             958..>2262
                     /locus_tag="VFC15_02615"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZM09084.1"
                     /translation="MRRIALIALILPLTATLLLAQAPQSSNAAKSTIPVPHVAALPPA
                     AEQAMASVDPEKIRAQVKFLASDVLEGRGTGARGGDIAAEYIATQFALYGLQPAGDNG
                     SYLQKVAMLGIATQDDSTFALVPDKGQPMNLRNRVDFVAMDETTKTVADVGASIVWIG
                     YGIDAPEFNWNDYKDVDVKGKVLLMMVNEPPSDDPKYFGGKALTYYGRWTYKYEQAAR
                     MGAVGVILIHKTDMASYGWNVVRNSWSGERAYLRDDRAPKLQVASWIQLDVAAKLAAA
                     CGMNLDTMMAAAQKPGFKAVPLPAKLKAHIVSTVRPFDSSNVVAKREGSDPKLKGEAV
                     IYSAHYDHLGIVPGMAGDNIYNGAQDNATGVAILLEIARAFAEAPQPPKRSVYFASVT
                     AEEQGLRGSEYLGLHPPIPAKDISLDLNYDDLPPLGVPEQVNV"
BASE COUNT          445 a          695 c          674 g          448 t
ORIGIN      
        1 tggacgtacg aaaacggtcc aatcaacggc aatgttgacg cttggaccat caacttcgga
       61 ttcacggtca gtgacagcta cctggctgcg ggtacgtcgg tcaacggttt tgagtttggc
      121 gtgcgggttg tcccgggaaa cgtggtcaac ggagtggact ggatcctcag cactggtccg
      181 tgctccgatc ccggcagcgg ttgtggaacc attgtgggct tcggtacggc caccagcaac
      241 ctgaccgacc aatttctgtt tattaacaac tttggctacg agatcgatct gatcacggtg
      301 tcgaacctca acgtaccgga aataggcggc caagggtact acctgactct ggccaacgcc
      361 actacggtcc agtcccttga gcctgtttac tgggacgaaa atagcggcgt cggctgccac
      421 tctgcaggct gcccgtcgac ggcctatgag aacaccctgg gcacgattcc ttcggaagct
      481 ttcaccattg gcggtggcag cggcggtgga accaccccgg aacccagcag catcctgctg
      541 ttcggttcag gcatcgtcgg gcttgcaggc gttctgcgtc gtaagctgaa ccgctgatga
      601 gtttcgaacg agcaccccgc gcatcatgtc tgtcatcctg agcggagcgc ccgcaaggaa
      661 tttccgaccc gcttctgttg cgggtcgggc gggcgcgaag tcgaaggatc tgcgtttgtt
      721 ttccgacgat gtaatggcgc gggcgaaccg cagatccctc gctggctgaa ctctacccca
      781 acaagcgcaa aaacggcgct tgttgcggac ctgttcgccg gctcgggatg acaaaagtaa
      841 gggagttgca acggcgcgcc taaaggcgcg ccccttcctc tcacatcccc ttctcccctt
      901 ttcgcgtaga atgatgagtt tctgcattca tccctgactc caccggagac ttcctctatg
      961 cgcagaatcg cactcatcgc ccttatcctt ccgctgacag caactctgtt gctggcccaa
     1021 gcaccgcaga gttccaacgc cgcgaagtca acaatcccag taccgcacgt tgctgcgctt
     1081 cctccggccg cggaacaggc catggcctcc gtcgatccgg agaagattcg cgcgcaggtg
     1141 aagtttctgg ccagcgatgt tctggaaggc cgcggcacgg gcgcgcgtgg aggcgatatc
     1201 gccgccgagt acattgccac gcagtttgcg ttgtacggac tgcagcccgc cggcgacaac
     1261 ggcagctatc tgcagaaggt ggcgatgctg ggcattgcca cccaggatga cagtacgttc
     1321 gctctcgtcc ccgacaaggg ccagcccatg aaccttcgta accgcgtgga cttcgtcgcc
     1381 atggacgaaa ccaccaaaac ggtcgccgac gtgggcgctt ccatcgtgtg gattggctac
     1441 ggcatcgacg ctccggagtt caactggaac gattacaagg acgtcgacgt caagggaaag
     1501 gtgctgctca tgatggtcaa cgagccgcct tcggacgatc ccaagtattt cggcggcaag
     1561 gcgctcacgt actacggccg ctggacctac aagtacgaac aggctgcgcg catgggagcc
     1621 gtcggcgtca tcctcattca caagaccgac atggccagct acggttggaa cgtcgtgcgc
     1681 aattcgtggt cgggcgagcg cgcctatctg cgggacgacc gcgctcccaa gttgcaggtc
     1741 gcctcatgga ttcagcttga cgttgccgcc aaacttgccg cggcgtgcgg catgaacctc
     1801 gataccatga tggcggcggc gcagaagccg ggcttcaagg cggtcccgtt gccggccaag
     1861 ctgaaggccc acatcgtcag cacggtacgg ccgtttgact ccagcaacgt agtcgccaag
     1921 cgggagggtt ccgatcccaa gctgaagggc gaagccgtca tctacagcgc gcattacgat
     1981 cacctgggca tcgtgcccgg catggcgggc gacaacattt acaacggcgc gcaggacaac
     2041 gccacgggtg tcgccattct gctggagatc gcgcgcgcct ttgccgaagc gccgcagccg
     2101 cccaagcgtt cggtgtactt cgcctcggtc actgcggagg agcagggatt gcgcggctcc
     2161 gagtatctgg gtttgcatcc ccccattcct gcgaaggaca tttcgcttga tctgaattat
     2221 gacgatctgc caccgctcgg tgtgccggag caggtaaacg tg
//
LOCUS       DASRKK010000116         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_886876, whole genome shotgun sequence.
ACCESSION   DASRKK010000116 DASRKK010000000
VERSION     DASRKK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886876"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFC15_02620"
     CDS             complement(<1..618)
                     /locus_tag="VFC15_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09085.1"
                     /translation="MDSSEDLLQNVYAYFLKAYRQGAPGAAQDELVAFESIGFSPGCS
                     ALTGNNVAAAALEEVSHFTDELPEVVGGVYTHTLRTISFTYGGMLDAAEPSSAAALAT
                     FSDFKSRAQKAYQGTIGSFDGPTPYLPTYPTPVDWYDLSNAANWQSYSYDASAKAPAP
                     AAPAPVAPNVRMQWRIVPQEMRPLILQQRNQEGPPAPGGVRHPMFM"
     gene            complement(629..2350)
                     /locus_tag="VFC15_02625"
     CDS             complement(629..2350)
                     /locus_tag="VFC15_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09086.1"
                     /translation="MASIPELVLIDLFNTLVDRIKQTQPARTDGKPLSGGLVYSQLVL
                     GMPIDPRDYGNPWSPMGGASGASQQQQTGVPVVAGQPDPKLLRALEAAFKTSQLCNIM
                     LQVTDDGSYLQYPVGRHLEFQYSGIVSAMQPLPVPPPPPDVQQAVNDAMNVLYTIDTT
                     DPANPVIEGKSPLYSKYTKNAIAYAKAKADYASAMAKTLADPTQANIWPQLSAPYQAT
                     VDDAWDTWKTEGADKVERALAVYESQGVNMQQGQIAKAKKLMDIWSLGLAGVPTDIPY
                     SYVDPSEWCNPDVDDIGFEQLTIKRTSEDHVASSVTDSNHSMYWNNEQTATSQSAHGG
                     FFGIGSASEASSNADQHSANGDASAYVFHQNLSNNFSDLEIDLEWALVSIYRPWLISD
                     LFYMNNWYIQGERANCISDGQIHTQVRSTTPMLPLIPQQMLVVRNVSIKSSNWGDNTA
                     TLNQLYGGDQGSADASQSSAGGSASAAYGPISFGGGVQHSDNKAQAQGSGYSAGSALT
                     NRQASFDGTTLTINGAQVIAFLSDIVPPSPSIDDPALPKPKKSAAGGATAGSSSPQPP
                     PSAQVPN"
     gene            complement(2559..>2808)
                     /locus_tag="VFC15_02630"
     CDS             complement(2559..>2808)
                     /locus_tag="VFC15_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09087.1"
                     /translation="ACDYGEESEERSDLGHGFFTKAIIDAVNACNFQISHTDLLAALR
                     KTVSGYVSTYSTPPQTPQLRGRPVRLEENFLAGWNYSI"
BASE COUNT          524 a          781 c          877 g          626 t
ORIGIN      
        1 catgaacatc ggatgcctga cgccaccggg cgctggcggt ccctcttggt tccgctgctg
       61 aagaatcagg ggccgcatct cctggggaac aatacgccac tgcatgcgca cgttcggcgc
      121 cactggcgct ggcgccgctg gagccggagc ttttgccgag gcgtcgtagg agtagctctg
      181 ccagttcgca gcattgctga gatcgtacca atcaactggc gtcggataag taggaaggta
      241 cggggtgggt ccgtcgaacg aaccgatagt cccttggtag gctttttggg cccgcgattt
      301 gaaatcgctg aaggtcgcga gggctgcggc cgaagacggc tcagccgcat cgagcatgcc
      361 cccatacgtg aaagagatgg tacgcagagt gtgagtgtag acgcccccca cgacctcagg
      421 cagctcgtcg gtgaagtgtg acacctcctc gagcgcggcg gcggcaacgt tgtttccggt
      481 aagggcgctg cagcccgggg aaaacccgat gctctcaaag gcaacgagtt cgtcctgcgc
      541 agcgcccggc gcaccttgcc gatacgcttt caagaagtac gcatagacgt tttggagaag
      601 atcctcgctg gagtccataa cggtcccctt aattcggtac ctgagccgag ggcggaggtt
      661 gcggcgacga tgagccggca gtcgctccgc cagcggcgga cttcttcggt ttcggcaagg
      721 ccggatcgtc gatgctcggt gaaggaggaa cgatatcgct gagaaacgcg atgacctgtg
      781 cgccgttaat cgtgagcgtg gtgccgtcga agctcgcttg acgattggta agcgcgctgc
      841 cagcggaata gcccgaccct tgggcttgag ctttgttgtc gctatgttgg actccgccgc
      901 cgaacgagat cgggccgtac gcggccgaag cgctcccgcc agcgctgctc tggctcgcat
      961 cggccgaccc ttggtcgccg ccgtagagtt gattgagggt cgccgtgttg tcgccccaat
     1021 tgctgctctt gatgctgacg ttgcgcacta ccagcatctg ctgcggaatg agcggcagca
     1081 tgggagtggt cgaacgcacc tgggtgtgga tctgcccgtc gctgatacaa ttcgcgcgtt
     1141 cgccctggat gtaccagtta ttcatgtaga acaggtcgct gatgagccac ggacggtaga
     1201 tgctgaccag ggcccattcc agatcgatct cgagatcgct gaaattgttt gagagatttt
     1261 gatggaacac gtatgccgat gcgtcgccgt tggcggagtg ctggtcggca ttgctgctgg
     1321 cctcgctggc actgccgatc ccaaaaaacc cgccgtgcgc cgattggcta gtagcggttt
     1381 gctcgttgtt ccaatacatg ctgtgattgg aatcggtgac cgaagatgcg acgtgatctt
     1441 ccgacgttct tttgatggtc aactgctcaa agccgatgtc gtcgacgtcc ggattacacc
     1501 attcgctcgg atcgacatag ctgtagggaa tatcggtcgg cacgccggca aggccaaggc
     1561 tccaaatgtc catcagcttt ttggctttcg cgatctggcc ttgctgcatg ttgacacctt
     1621 gcgattcgta gaccgcgaga gcgcgctcga ctttgtcggc gccttcggtc ttccacgtgt
     1681 cccaggcgtc gtccacggtt gcctggtacg gggccgagag ctgcggccag atgttggcct
     1741 gcgtcgggtc cgccagcgtc ttggccatgg cgcttgcgta atcggccttc gccttcgcat
     1801 acgctatcgc gttcttcgtg tacttgctgt agagcgggct cttgccttcg atgacgggat
     1861 tcgcgggatc ggtcgtatcg atcgtgtaga gcacgttcat ggcatcgttg acggcttgct
     1921 ggacatcggg cgggggcggc ggcactggca agggctgcat cgccgaaacg atgccggaat
     1981 actggaattc caaatgccgt cccacgggat attggagata cgatccgtcg tcggtgactt
     2041 gcagcatgat attgcacagt tgcgaggtct tgaacgccgc ttcgagtgcg cgcagcaatt
     2101 tgggatcggg ctggcctgcg acgaccggca caccagtttg ctgctgttga ctagcaccgc
     2161 tcgcgccgcc catcggtgac caagggttgc cgtaatcgcg cggatcgatc ggcatgccaa
     2221 gcacgagttg cgaatagacc aggccgcccg acaacggttt tccgtcggtg cgcgcgggtt
     2281 gcgtttgctt gatgcgatcg acgagcgtat tgaaaaggtc gattaacacg agttctggaa
     2341 tactagccat gtgatggtcc ttctgccgtc gcagcgatgc gcggcgattt ggtgaggttt
     2401 aggagatacc gaagcggtgc aaagaattgc atgagacggt agcatccaat gtattgcctg
     2461 tcaaccgtga aacttgtgtt gtcgcagcac ttcattacag ctccgatcgc cactcagatg
     2521 gagtggattc cttcgcgaca cttagttccc tgcaagtgtc aaatgctata gttccagccc
     2581 gccaggaagt tttcctctaa tcgcaccggt cgcccgcgta attggggagt ctgcggcggc
     2641 gtgctatagg tcgagacata gcccgatact gttttgcgca gcgctgccag caggtccgta
     2701 tgactgatct ggaaattgca cgcgttgacc gcgtcgataa tcgccttggt aaagaaacca
     2761 tggcccagat cggagcgttc ttccgactcc tcgccataat cacaggcg
//
LOCUS       DASRKK010000117         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_398344, whole genome shotgun sequence.
ACCESSION   DASRKK010000117 DASRKK010000000
VERSION     DASRKK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398344"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1503)
                     /gene="hpnI"
                     /locus_tag="VFC15_02635"
     CDS             complement(349..1503)
                     /gene="hpnI"
                     /locus_tag="VFC15_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZM09088.1"
                     /translation="MHSALTSVAVLCALLALAGAAYFAMCIVAAMRFRRECSHPSETL
                     FTPPVSILRSLKGLDPHMYSAFRSHCLLDYPEYEVLFGVSEADDPALALVEKLGEEFP
                     QAKLRVVHCPQVLGLNGKVSNLAQMLPQASYEYIIINDSDILVPRNYLLRVLAPLAQP
                     GVGMVTALYRGLAGKTLGSKLEALGLSTDFSGGVLVARAMEGGIRFALGATIATTKTV
                     LREIGGLEPLADYLGDDYELGARAAAAGYQVRLADIVVETALPDYSFRDFWAHQLRWA
                     RNVKDRRGAQYFGLIVTFGLVWAVLAVVAAPRAWWTWLVFVVTAALRVMSAVVVGRGV
                     LDDPQVLRDLWLLPLRDVVALAIWLVSYFGDEVEWRGLRFRLRDGKLERI"
     gene            1640..>2383
                     /locus_tag="VFC15_02640"
     CDS             1640..>2383
                     /locus_tag="VFC15_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM09089.1"
                     /translation="MINGKRVAVVMPAYNAESTLEATVREIPELVDIRILVDDYSKDK
                     TAALARKLGLTVFEHDRNLGYGGNQQTCYREALAAGADVVVMVHPDYQYTPLLITALA
                     SMVAYDVYDVVLGSRIIGGQALHGGMPLYKYIANRFLTAFENLFLRVKLSEYHTGYRA
                     FSRKVLTELPLLENSPDFVFDNQMLAQCVHFGFRIGEVSCPTKYFEEASSINFRRSVT
                     YGLGVMGTTLQFALHHWGLVRLPRFSPEGR"
BASE COUNT          541 a          708 c          695 g          439 t
ORIGIN      
        1 ggcgtgtggg ccgccagctt tgccggcaag acattcttct ggcgcgggga aacgtatcag
       61 tttggcaacg aaggcaggat tacgcccgag cagcgtccca tcagcttgct tagtcacggc
      121 ttgccgcaag ggcacgacta gtgcagtttc acactggatg aatagggtgg agcaccgctt
      181 cagcggtgca attacgattc ggagacgaac cggctttagc cgctgaggtg atggaaacat
      241 tacctcaggc gctaaagcgc gatcccagaa ctgacggcag ttatgcacgc ctgaaggcgt
      301 gctccaccca tcgtctatac accagatgtg aaactgctat aaactgcgct aaatccgttc
      361 gagctttccg tcgcgaagcc tgaagcggag cccgcgccac tccacttcat ctccgaagta
      421 gctgaccaac cagattgcca gcgcaaccac gtcacgcagc ggtagaagcc atagatctcg
      481 caagacttgc ggatcatcca gcaccccccg gccaaccacg acggctgaca tgacgcggag
      541 agcggccgta acaacaaaga ccagccacgt ccaccatgcg cgaggcgcag ccaccaccgc
      601 gagcacagcc caaaccagcc cgaaggtcac gatcaatcca aaatactgcg cgccgcggcg
      661 atctttgacg ttacgcgccc agcgcagctg atgcgcccag aaatcgcgaa aactgtaatc
      721 gggcagcgca gtctcgacca cgatatcggc caaccggact tgatatccgg cggcggcggc
      781 gcgcgcaccg agctcgtaat cgtcgcccag gtagtcggcc aagggctcca gaccgccgat
      841 ttcgcgcagt acggttttgg tggtcgcgat ggttgctccc agtgcgaaac gaatgccgcc
      901 ttccatagcg cgtgcgacca gcacgccgcc actgaagtcg gtgctgagcc ccagcgcttc
      961 gagcttcgag cccagcgtct tgcctgccag gccgcgataa agcgctgtga ccatgccgac
     1021 tcccggctgc gccaacggcg caagcacgcg cagcaggtag ttgcgcggga ccaggatgtc
     1081 gctgtcgttg atgatgatgt actcgtagct ggcctgcggt agcatctgcg caagattgct
     1141 gaccttgcca ttgaggccga gcacctgcgg acaatgcaca acgcgcaact tcgcttgcgg
     1201 aaactcctca cccagtttct cgaccagagc cagcgcggga tcgtcagcct cgctcacgcc
     1261 gaacaacact tcatattccg gataatcgag caaacaatgg ctgcgaaaag cgctgtacat
     1321 gtggggatcg agccctttta gagacctcag gatgctgacc ggaggagtga agagcgtttc
     1381 gctggggtga gagcactcgc gccgaaagcg catggccgca acaatgcaca tcgcgaaata
     1441 agccgcgcca gccaaagcca gcagcgcgca aagcacggca acagaggtga gcgcagaatg
     1501 catggagagc ttttcagatt agcagaacca tgcgctgcgc tgacatcggc gaggctaccg
     1561 ccaggtcttc tacgaggaaa cgatcgaggg cgcggtatta tgtaccagat gttgcgagat
     1621 acccgtcaag agagcctaaa tgatcaacgg taaacgggtc gcggtggtga tgcccgccta
     1681 caacgcggag agcacgctgg aagcgacggt gcgggagata cccgaactgg tggatatccg
     1741 catcctggtg gacgattaca gcaaagacaa aaccgcggca ttggccagga agttgggact
     1801 gacggtgttc gagcacgacc gcaaccttgg ctatggcggt aaccagcaga cctgctatcg
     1861 ggaagccctc gcggcaggcg ccgacgtcgt ggtcatggtc cacccggatt atcagtacac
     1921 gccattgctc atcaccgccc tggcaagcat ggtggcctat gacgtgtatg acgtggtgct
     1981 ggggtcgcga atcataggtg gccaggcgtt gcatggcggc atgccgctgt acaagtacat
     2041 cgccaatcgt tttctgacgg ccttcgagaa cctgttcctg cgcgtaaagc tctccgaata
     2101 tcacaccggc tatcgggcgt tcagccgcaa agtcttgacc gagctgccgc tgctggaaaa
     2161 ttctccggac tttgtgttcg acaaccagat gctggcgcaa tgtgtacact tcggatttcg
     2221 catcggcgaa gtgtcttgtc caaccaagta ttttgaagag gcgtcttcca tcaattttcg
     2281 ccgtagcgtc acctacggtc tgggcgtaat gggcacgact ctccagtttg cacttcacca
     2341 ttgggggctg gttcgcctac cgcggtttag tccggaagga aga
//
LOCUS       DASRKK010000118        24787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1277877, whole genome shotgun sequence.
ACCESSION   DASRKK010000118 DASRKK010000000
VERSION     DASRKK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 24787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24787
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277877"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFC15_02645"
     CDS             complement(<1..657)
                     /locus_tag="VFC15_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM09090.1"
                     /translation="MKKDFTRRNFLRTAAGAAGLALATKAIALPEPAYAQQWATGPSG
                     EMVQFGIIGIGMQGSGLLKASVELPGTKCLAACDLWDGRQTLAKEIAGPNITTTRKYQ
                     ELLANKDITALIVAVPDHWHKQIVVDAVTAGKDVYCEKPMSHSVADGAAMVAAAKKSG
                     RIVQIGSQRVSSALLAKAKQLYDQGAIGELLSVELSLGRNDPTGAWEYPPPPGLAPDN
                     F"
     gene            complement(730..1422)
                     /locus_tag="VFC15_02650"
     CDS             complement(730..1422)
                     /locus_tag="VFC15_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZM09091.1"
                     /translation="MKRLTFLAILLLFALPLLAQSDGWIQLFNGKDLTGWKHVGPGEM
                     LVENGLMRTHGGMGLLYWTTGPLGNCVIRVVFKMRDENDNSGVFIRIPIEPREPWMPV
                     HYGYEVQIDNHPETSGEDEYHVTGTLYSLTKPLAKPGKPGPQWNTMEITLDGPHTIVM
                     LNGVKVTDYTEGDPVPERKFWFEGTRGRRPNFGWIGLQNHGNYDEQKGKYSDENADVV
                     FFKEVAMKPLTK"
     gene            complement(1807..2031)
                     /locus_tag="VFC15_02655"
     CDS             complement(1807..2031)
                     /locus_tag="VFC15_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09092.1"
                     /translation="MEISFAPELEAKLKQIASQTGKGADQVVQEVVASYFDHDEWFKQ
                     EVEKGLASLDRGKFVSHEEVRRQMERALGS"
     gene            complement(2110..3450)
                     /locus_tag="VFC15_02660"
     CDS             complement(2110..3450)
                     /locus_tag="VFC15_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZM09093.1"
                     /translation="MRNHSPHRTTRLAPCVAIALAMVVFSLALTAQSSYQPSAENLKS
                     REWFEEARFGMFIHWGIYSELGDGEWVMQEHHLTGPQYEQLAAQFYPTKFDAAQWVAL
                     AKQAGMRYIVVTSRHHDGFSMFATKQNKYNVVDATPYGKDVIRQLADECHRQGIKLFV
                     YYSQLDWHHHDYYPLGGTGHWEGRPTNGQWPRYLDFMNAQLTELFSNYGELGGVWFDG
                     MWDKPDADWQIDRTYALIHKLQPGVLIVSNHHKLPFPGEDYQTFEKDLPGDNTTGWGG
                     APIGKLPLETNDTMYRGGSWGFNLRDMDPKSLRDLVQYMVRAAGNNANFLLNVGPQPN
                     GEIQTAFADRLREMGAWLKIYGESIYGTRGGPVKPGGWGVTTQRGNVIYVHVLDPSLS
                     VLALPGIAKPVRDAHLLNGGSKVSYGISEGSVVLQLPKRTPGEIDQVIVVELAE"
     gene            3582..4034
                     /locus_tag="VFC15_02665"
     CDS             3582..4034
                     /locus_tag="VFC15_02665"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM09094.1"
                     /translation="MNDRIEKHIELKAPVSRVWRALTDYREFGEWFRVKLDGPFVPGQ
                     ISRGQLTWPGYEHVVWEAVVQTMEPERLFSFTWHPYSVDPKIDYSKETPTLVEFTLEQ
                     IPGGTLLVVTESGFDKIPSDRRDEAFRRNDGGWAQQMENIAKHVAPTS"
     gene            4015..4362
                     /locus_tag="VFC15_02670"
     CDS             4015..4362
                     /locus_tag="VFC15_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZM09095.1"
                     /translation="MSHQRRNRLADKRQARAPVFGALGDETRLLLVAKLSGGRPCSIS
                     QLTLGSKLTRQAITKHLRVLESVGIVRGVRKGRENLFEFDPQPLAGMKEYLDSVSEQW
                     DQALARLKSFVED"
     gene            4793..5566
                     /locus_tag="VFC15_02675"
     CDS             4793..5566
                     /locus_tag="VFC15_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09096.1"
                     /translation="MNRKMWGVFAGLCALAMSAVCAAPAVAQVSEKPPMYSYVGDWAI
                     PRAQWAEMEKSATADQSILDKAIASGTIIAYGNDTNLVHHPEGMTHDDWWSSMSMAGL
                     VNVLDQFYKSGNSVSPVLSSATKHSDDIFVSRYYNYHAGSWKGLYTHVSTYKLKADAP
                     DDAVELLSKNIIGPVLDKLLTEGAIHEWEIDTQAIHTEAPGTFWVVYIAANAEGLDKA
                     SAAVREAGKANPLRGPTFNSVVDYTAHRDMLARTNATYK"
     gene            complement(5745..8624)
                     /locus_tag="VFC15_02680"
     CDS             complement(5745..8624)
                     /locus_tag="VFC15_02680"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM09097.1"
                     /translation="MAGPSRYLIGIDLGTTNSAVAYVDTTEGAGPAESSGIHTFDVAQ
                     LTAPGEVRAEPLLPSFLYFPTADEIVSGAVNLPWEERPSSIVGVMAREQGALIPGRQV
                     SSAKSWLCQSAVDRTANILPREAEPPEPMVSPVEASARYLTHLRNAWNHAMAADGVTD
                     PQQLFENQEIVLTVPASFDEEARELTVEAARQAGLKNLTLLEEPLAAFYAWVAEQQNG
                     LSEQLRDGDLALICDIGGGTSDFSLVRAHVVGASVEFERTAIGEHLLLGGENLDLASA
                     RRVEQKLNAKLSLRQRHALRIACSAAKERLLGDEGIDRLPINILGGGRSVVGQMLSSE
                     LTHDEVVELLTSGFLPLTARDDIPARARAPGLRELGLPYASDPAITKHLAAFLRRAAM
                     AMRDGKSSQSRNDDRASMVRPDSILFNGGFCIPAIARERIADAIANWFSASEGWRPKV
                     LRNEVMSSAVAIGAAYYGRVRRGAGVRVKAGSARTYYIGMRSEQGIKAVCVLPSGTNE
                     GTTLPLLDREFSVLANRPVSFHLYSSTVRHDAHGEIAELDPDEVHRHAPLVTLLRYGK
                     KLQQMELAVRLSVSFTEVGTLELWCESASSAHRWRLQFELRRETSANDQLGSAAQRTS
                     TAHLSALMPQESQEEAVQLIKVAFRKSAAGVGDAIDPAALVSELESVTDLKRESWPLA
                     TARLFCDALLEVADGRKLSPRHEVRWLNLLGYCLRPGFGDTQDNARITQARRIYSSGL
                     VFPRELQSQVDWLVLGRRIAGGLGAAHQHEIRNYLGDLGIGRKKPSTRLNSQVEHDGW
                     RLLASLEHLSGATRAALASELLRKLKKEPADSGWLWSLGRFGARIPLYGSLSCVVPSE
                     SASEWIAALLELGELTHETASAILQLGRRVEDRTRDIDADVARSAVAKLKGAKIADDA
                     LMRPLLEYVAPGRPDVVRTFGEPLPKGLELESTANCLSPVTALTT"
     gene            complement(8653..10500)
                     /locus_tag="VFC15_02685"
     CDS             complement(8653..10500)
                     /locus_tag="VFC15_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM09098.1"
                     /translation="MDSRFIVGIDLGTTNSALAHCDTAAAAESARIEVGAIPQVVNPN
                     EVADRTLLPSFLYIPGELDFPKSSLSLPWDADPKFVIGELARKRGAENPGRLVASAKS
                     WLSYAAANRTSPILPWQAPDEVSKLSPVEVSSQYLQYLRTVWDTQHAADGKGLTLGEQ
                     DVLLTVPASFDEEARELTRRAAEQAGLERVTLLEEPQAAFYAWLESQGDSWRQRIKVG
                     DLVLVCDVGGGTTDFSLIAVSEEDGDLALKRVAVGEHILLGGDNMDLALARLLQQKLE
                     AEGQKTDTFQLQALWHQCRAAKEYLFEHPKSQKQDVTLLGKGRKLVGGTIKTELLRED
                     LNRVLVEGFFPKVASDEMPARQRRIGFQELGLPYAADAAVTKHLARFLSQQVQNSPEA
                     ASIRRGRSGLACPTQVLFNGGVMKADVLRGRLVEVLSSWLVAEGFEALGAAQILDAPD
                     LEHAVARGAAYYGRARRGQGVRIRSGASRTYYVGIETAMPAVPGMEAPLKALCVVPFG
                     MEEGSEALVPGREFGLVVGEPAEFRFLSSTIRKQDHVGDLLENWGSDIEELSPLEVTL
                     TLEGQQGTVLPVRLESRVTEIGTLELWCVSRDNANHWKLELNIREKSDQ"
     gene            complement(10566..11096)
                     /locus_tag="VFC15_02690"
     CDS             complement(10566..11096)
                     /locus_tag="VFC15_02690"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZM09099.1"
                     /translation="MIGFSTRLSFAFRCFFSLLFQGMIPQDILETLSVSKSPAVNSLG
                     DKPAPESLDRAVQVLTLFQRDGRLVDFLTEDVSPYPDSQLGAAVRSIHASCRQVLDRY
                     LKLEPVLSSEEDQPVTVPVGFDPAAIKLVGNVAGEPPLRGLLRHRGWRVTEINLPSLP
                     QGSGRAIVAPAEVEIP"
     gene            complement(11331..12287)
                     /locus_tag="VFC15_02695"
     CDS             complement(11331..12287)
                     /locus_tag="VFC15_02695"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM09100.1"
                     /translation="MKKYVVTGGAGFIGSALVRALLALGDGSVEVLDNLTTGRRQNLA
                     EVSSQIVFHQVDICNFDAIRPVISAADTVFHIAAIPSVPKSIVDPVPSHESNVDGTFN
                     VLRACVEGGVRKVVYAASSSAYGDTEVLPKVETMSPNPKSPYAAQKLMGEYYCSAFSS
                     CYGLDATSLRFFNVYGPRQDPGSPYSGVISVFMTSLLERTSPTIYGDGEQTRDFTYIE
                     DVVDLVIKAAKAPRVAGKVFNAGNGNRYSLNQVWNLLQKIEGVEIPTLYRASRAGDVR
                     DSQADTTAAVKELGHAPRFTLEEGLRRTLEWYRESLVSTGRP"
     gene            12799..13815
                     /gene="xrtA"
                     /locus_tag="VFC15_02700"
     CDS             12799..13815
                     /gene="xrtA"
                     /locus_tag="VFC15_02700"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HZM09101.1"
                     /translation="MIKLALETTNSTENPVAVASENAPVEGGGGPKPPTLWERIQTFG
                     RQLQSFGWRTWLLVAVMIGLYGPVLVRLVRQWNNDADYSHGFLVPFLSAYLIWQRRDK
                     LAEVDRRPSNWGLIVVVFSLGLLFLGSLGAELFLTRISIIFTICGLIVYFSGWRLLRA
                     LAFPLAFLLFAVPIPTLIYNEIVFPLQFIASKFATSVLEMLNLFPIMREGNVLIMPGM
                     RLEVVEACSGIRSLMSLLALAAGYGYLAEKSVPVRWFMFLAMVPLAIVSNGTRVMITA
                     LMTNYIGPRAAEGFMHEFSGWVIFVVATALFLALHSLTIVVRKKLGWMPKDAAVAAKG
                     QVKE"
     gene            13812..14453
                     /locus_tag="VFC15_02705"
     CDS             13812..14453
                     /locus_tag="VFC15_02705"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HZM09102.1"
                     /translation="MSNAVRYWTMIAVLLAATAGVGFLSHGESTPPARPLTDFPKDIG
                     NYKTVAEIPFDQETLTILGVTDYLNRVYFSPTQGELGLYIGYFRTQRTGAQIHSPKNC
                     LPGAGWQPVVSETYQLALDDGRKVPINLYVIRKDLDQEIVLYWYQSHGRVVASEYWGK
                     FYMVYDAIRLNRTDAALVRITAPVRNGDLDAARTQALAFAKQASADVDQIIPR"
     gene            14467..16752
                     /locus_tag="VFC15_02710"
     CDS             14467..16752
                     /locus_tag="VFC15_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09103.1"
                     /translation="MRNRYVISIATALLAVLLVGATGCKSNPEKAKKKYLESGLSYVD
                     KKQYDAAVIQFKKALQVDPRYADAHYQLGLAYLRQQKVKEGYAELRQAIDLDPKNVKA
                     QLELGNILWSVKEFKRSEDAARKVIELDPNSPEGYTLLGTALFAEKDPEGALQAYNKA
                     IELKPNDAGVYLNRGVLYTSMKKDAEAEADFKKAVSLNPKNLEGYSNLSRFYQYKQEP
                     AKAEEVLQEAIKNDPDSPANYLRLAGMLLQEKRLADAETVIGNLRNRLPKNTDVAGAI
                     AEFYLAARNPDAAIKEYQRGLAIDPKNQKLTVALGDAYLMSGHVEEATKLDEQMLKNK
                     PNDVPGRMLRGRLEAIRGDFPTAVTTFRGVVKDSPEDPQAHYFLGQALLKTGDLNGAK
                     TEFQEAIKRNPDNPLYLQAQAEAYRESRDFTTAKEFASRLLKLNPNNPQTHFLLASID
                     LGAKEYQSALEELSIVAQAAPNDPTVHLNMAFAYGGLKKYPEAEREFQTAMKLNPQFD
                     GAMGEYVAMLFGLNQGPKALQVANQYATANPNRAAAQFIYASALANSKKLDEAIPEFQ
                     KAIQLDPKAILSYMQLARVYEMQNKLDDALATFQKALAVAPNNVAIVGAIGNVYLARN
                     DPKTAQQYFEKANTLAPHDPYIQNNLAWVYAVQGQNLDVALSLATQAKQAAPDAIAIN
                     DTLGWIQYKKGNYVIAVGLLTEVVNKLPQSAEYRYHLGMALNAAGQKDRAKEELKKAL
                     QLTPPLNHDDTGQAQTTLAKL"
     gene            17010..17441
                     /locus_tag="VFC15_02715"
     CDS             17010..17441
                     /locus_tag="VFC15_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09104.1"
                     /translation="MNLISSVVSWIAIAGVFVVVWVILRRERILSALAIVGIAGILLS
                     NRYLWHLGRPHLDTVLPILSDFVPIAMALVGIIMSYKQPKRESHLWTTVILLSAGFIG
                     TGILSWTRIRNEAAHKKEVETLSNKVDAVGTQNSSILGQIV"
     gene            complement(18307..21489)
                     /locus_tag="VFC15_02720"
     CDS             complement(18307..21489)
                     /locus_tag="VFC15_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM09105.1"
                     /translation="MRFGLRISILVAVLAATATAQTFRGAIHGSVEDPTGGMVANAQV
                     KATDTATGVEHATVTTSGGEFTFQDIPLGTYQVTVTASGFNKATFDKVPVTAGSIYTL
                     PVKLSMGAEATTVEVSAAALTVDTTSATQTMTISDTSVQDTPLNGRDFSQLISVSPGY
                     GGYSVGGFGSLNGTRANQMNWQIDGTDNNDMWHNIPAVNQGGVSGIAGVIMPIDAVQD
                     FSAQTQSNAENGRNAGGIVNVVIKSGANQVHGSAYYYNRNGAYSANSPFYIATPEFPK
                     APPLTNQNVGGVIGGPIIKNKLFWFVGFEYQTYKLGLSGLSTEPSIAWANQAVALMNQ
                     YGVAVNPLSQTLLTTLWPSSVANLPATLNNYFSPVAGTGYSYNGVAKIDWTINAKNTL
                     SAHWFAGQGSQTQPPGLSLALATASSNLGYYFETAPLHVQNYNVVLNSVLTPTLTNQV
                     LAGVSYFQQSFHDANNTFNMKALGLWLSPDALINGNPIFGASNIGISGFGQVGITPPE
                     GRQDVTGHLTDVLSWSKGKHQFRFGGEYRDGHVDEYYYRRSLGSFTFDGTQGPWAGTC
                     ASTDPTCALADYLAGYVSSSSIAVGHAERAVVSHGYSLFTQDNWQVTRNLNLTLGLRY
                     EYFGPLHNGDKNLAVWIPGQGFKIQGADISSIFPADHNNFAPRAGFAWNPYGNGDMVV
                     RGSFGVFYDQINMNPFLDFRPPISGADGLQDNPVGPNPVSNYSLNNYQWAAGQYIFPG
                     VATCPTLSGCNQTFNVYSVNQNFRTPYFYNYNLQLEKAIGDKMVFQFGYVGSAAHKLN
                     VMLNINQNLAFNAAYPDIGSVLQLNSNGTSNYNALQTVLKMNSWHGLTGQFSYTWAHA
                     IDQVTEYRGVIPFDSFNIALDKGNSDFDTRQLFVGYLVYTIPGSSKGPEMLTHGWQLA
                     GNVNFHTGQPFNFNAGTQRPGMNVVCDPFAGVSHSFSAALGGEQWVNPACFVPAVGAG
                     NLARNRYYGPGFSDVDISVIKNIPIKERLTVQIRAEMFNIFNRINLASGAGSVGSNGF
                     VSDTIGDFNGSPGIGPGEPFNMQLAGKIIF"
     gene            22178..22408
                     /locus_tag="VFC15_02725"
     CDS             22178..22408
                     /locus_tag="VFC15_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZM09106.1"
                     /translation="MKAYVVVTARAGTARDIADAMAALPGVQMADACWGGGDVYAVVD
                     FPDWKDLNGLVLDKVHSMPGVIRTETHVAVER"
     gene            complement(22513..23976)
                     /locus_tag="VFC15_02730"
     CDS             complement(22513..23976)
                     /locus_tag="VFC15_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZM09107.1"
                     /translation="MGLNAIDLSVIAVYLTGVTAFGLRFRKRQRTLKDYFLADKQIAW
                     WAISLSIVAAETSTLTIISVPGLAYEKDFRFLQLVIGYLAGRFLICLVLIPQYFRGEL
                     VTAYQLMERRFGQKLRALTAGLFLITRAAAEGVRVFAVAIVVGIALSSLLAGLGDFAR
                     DVSAIAIVTLLTLIYTFEGGMAAVIWTDVVQLAIYISGTVAGFFTLLHLVPGGWSTVQ
                     SIAGEAGKFRVFDFSWNFYSTYTFWSGLIGGAFLTTASHGTDQLIVQRLLAARSERQS
                     KIALMTSWIVVMAQFSLFLVVGAMLFVFYKLFPPAVPFTRIDTIFTIFVVTKMPHGIS
                     GLLISAILAAAMSNLSAALNSLSSTTVVDFYGRWRPQSTEEHRVRLSRFATIGWALVL
                     FVLAMLARHGGKVLEVGLSIASVAYGSLLGVFLLGVLTKRTSERGAMVGMVFGFLLNV
                     YLWLFTRVPFTWYVAMGSCATMIVGYMVSRLLSRSPA"
BASE COUNT         5395 a         7144 c         7103 g         5145 t
ORIGIN      
        1 gaagttgtca ggcgcgaggc ccggaggcgg aggatactcc cacgcgccag ttggatcgtt
       61 acgtcccagc gagagttcaa cagacagcaa ttccccgata gctccctgat cgtaaagctg
      121 tttggccttc gccagcagtg cagaacttac gcgctgcgag ccgatctgca cgatgcggcc
      181 cgactttttt gctgccgcca ccatggcggc gccatctgcc acactgtggg acatcggttt
      241 ctcgcagtac acgtctttgc ccgcggtgac ggcgtccacc acgatctgct tgtgccagtg
      301 gtccggcacc gcaacgatca aagcggtgat gtccttgttg gcgagtagct cctggtattt
      361 gcgcgtggtc gtaatattcg gtccggcaat ttctttggcc agcgtctgcc ggccatccca
      421 gaggtcgcac gcggcgaggc acttcgttcc cggcaattcg acggacgcct tcagcagtcc
      481 agagccttgc atgccgatgc cgataatgcc gaattgcacc atctcgccgc tgggtccagt
      541 ggcccattgc tgggcgtagg ctggctcggg caacgcaatg gctttggtag cgagtgcgag
      601 tccggcggct ccggcggcgg tccgaagaaa attacggcgg gtgaagtctt ttttcatggc
      661 agataacgcc tcctgcgatg gcgggaaaca cgtttaactg ttgacctgca gtgcagcgac
      721 gagcgcgagt tatttggtta gcggcttcat ggcgacctct ttaaagaaaa cgacgtcagc
      781 gttctcgtcc gaatacttgc ctttttgttc gtcataattg ccgtggttct gcaggccgat
      841 ccatccgaag ttgggccgcc gaccgcgcgt gccctcgaac cagaacttcc tttcgggcac
      901 cgggtcgcct tctgtgtaat cggtgacttt aacgccattg agcatgacga tggtgtgcgg
      961 gccgtcgagc gtgatctcca tcgtgttcca ttgcgggccg ggcttacccg gtttggcgag
     1021 aggtttggtc aaggaataga gcgtgccggt gacatgatac tcgtcttcgc ctgaggtttc
     1081 ggggtgattg tcgatctgga cctcgtagcc atagtggacg ggcatccagg gctcgcgtgg
     1141 ctcgataggg atgcgaataa agacgccgga gttgtcgttt tcatcgcgca tcttaaaaac
     1201 gacacggatg acgcagttgc ccaacggacc agtggtccag tagagcaggc ccatgccgcc
     1261 gtgggtccgc atcaggccgt tctcgacaag catttcgccg gggccgacgt gcttccagcc
     1321 ggtgagatct ttgccattga agagttggat ccagccgtcg gactgtgcga ggagtggaag
     1381 agcgaacaac aaaagtatgg cgaggaatgt gagccgtttc accgtgaagc ctcccggatc
     1441 aagccgccgg acaaacacga attgtccgca gcggagaatg atgctccgag ggacgggatg
     1501 ttggcaagtc agtaaatcaa atctggggta cccgctgaac ccttatggcc agcgttgtga
     1561 gccatgcagc actctcacga tttccacgga acgttgtttt acgcggtaca ccagaatgaa
     1621 cggaagcggg gtcaagacca gttcgcgggt gtctctgatt ctccccggtc gcccgcgatc
     1681 agcaaatgat ttgagcgaag tgacgctttc aaagatggcg tgcgcaacgc gatcagcggc
     1741 tgaagggttt tgccggtgga tgtaacccac aattcccggc ggcttcaggc gaccagagaa
     1801 tcttcatcag gagccgaggg ctcgttccat ctggcggcgg acttcctcgt gtgagacgaa
     1861 cttgcctcga tcgagcgagg caagaccttt ctcaacctct tgcttgaacc actcgtcgtg
     1921 gtcgaagtag ctcgcgacca cctcttgcac gacctggtcc gcgcctttac ctgtctgcga
     1981 ggcgatttgc ttcaacttgg cctcaagctc gggtgcaaag gatatttcca tcagttcatt
     2041 gtaggagcga tcggatggaa ctttcaacga agattggagc ttgccagtgc ccggctgcac
     2101 ccgatctcgc tactccgcca gttccacgac tattacctga tcgatttctc caggggtgcg
     2161 tttcggaagt tgcagcacca cacttccttc agatatcccg tagctcactt tggacccccc
     2221 gttcagcaga tgagcgtcgc gcacgggctt tgcgatgccc ggcagcgcca acacactcag
     2281 cgacggatcg agcacgtgga cataaataac gttgccgcgc tgggtggtta cgccccatcc
     2341 ccctggcttg actggaccgc cgcgcgtgcc atagatggac tccccgtaaa tcttcagcca
     2401 tgctcccatc tcgcgcaggc gatcggcgaa ggcagtctga atttcaccat tgggttgcgg
     2461 gccgacgttg agcagaaagt tggcattgtt gcctgcggcg cgaaccatgt actgcaccag
     2521 gtcgcgcaag ctcttgggat ccatgtcgcg cagattgaag ccccacgaac cgccgcgata
     2581 catggtgtca ttggtttcca gcggaagctt gccaatcgga gcgccacccc atcccgtcgt
     2641 gttatctccc ggcaagtcct tctcgaaggt ctgataatcc tcgcccggaa atggcagctt
     2701 gtgatggttg gagacgatca gtaccccggg ttgcaactta tggatcagcg cataggtgcg
     2761 gtcaatctgc cagtcggcgt cgggcttgtc ccacatgccg tcgaaccaca cgccgcccag
     2821 ctcgccataa ttgctgaaca actcggtcaa ctgcgcgttc atgaagtcga ggtagcgtgg
     2881 ccactgtccg ttagtggggc ggccttccca atgcccggtg ccgcccagcg gatagtagtc
     2941 atgatggtgc cagtcgagtt gcgagtagta cacgaacagc ttgatgccct ggcgatgaca
     3001 ttcgtctgca agctggcgaa tgacgtcttt gccatagggc gtcgcgtcca ccacgttgta
     3061 cttgttttgc ttggtcgcaa acatggagaa gccgtcgtga tgccgcgagg tcacgacgat
     3121 gtagcgcatg cccgcctgct tggccagcgc gacccactgc gccgcatcga acttggtggg
     3181 ataaaactgc gccgccagtt gttcgtattg cgggccggtg aggtggtgct cctgcattac
     3241 ccactcgcca tcgccgagct cgctgtagat gccccaatga atgaacatgc caaacctcgc
     3301 ctcctcaaac cattcgcggg atttcagatt ttctgcagag ggttgatagg aagactgcgc
     3361 ggtcagcgcg agggagaaca cgaccatcgc taaagcgatc gcgacacagg gagcgagccg
     3421 ggtggttcga tggggcgaat gattccgcat gggaaacaga ttataggaag aaggcccgtc
     3481 ggactgcttc gcgccagcca aagtgtgtcg gtacgaatgg caaccaaaag gttgcttttc
     3541 tgtcgagacg tcgctaatat gcaacccaga ggttgcttaa catgaacgac cgtattgaga
     3601 agcacattga actcaaggca ccggtttcgc gagtttggcg cgcgctgacg gattaccgtg
     3661 agtttggcga atggtttcgg gtgaaactcg acggcccctt cgtaccggga cagatctcgc
     3721 gtggccaact tacatggccg ggctacgaac acgttgtatg ggaagccgtg gtgcagacga
     3781 tggagcccga acggctcttt tccttcactt ggcatcctta ttctgtcgat cccaaaatcg
     3841 attactcgaa ggagacgccc acactcgtcg aattcactct ggagcaaatc cctggcggca
     3901 cgctgctggt ggtcaccgag tcgggcttcg acaagatccc aagcgaccgc cgcgacgagg
     3961 cattccgcag gaacgacggc ggatgggcgc agcaaatgga gaacatcgcg aaacatgtcg
     4021 caccaacgtc gtaatcgctt ggccgataag cggcaagctc gcgcacccgt atttggcgcg
     4081 ctgggtgacg aaacgcgcct gttgctggtt gcgaagctat ctggtgggcg gccttgttcg
     4141 atctctcaac ttacgcttgg ttccaaattg acccggcagg cgatcaccaa gcatctccgc
     4201 gtgctggaga gcgtaggaat cgtgcgtggc gtccgcaaag ggcgcgagaa cctgtttgaa
     4261 ttcgatccgc agccgcttgc aggaatgaag gaatatctcg attcggtttc cgagcaatgg
     4321 gaccaagcgc tggccagact gaagtcattc gtcgaagatt gagcgcttcg accggcgatg
     4381 aatcgcacga agcaaacctc aaagcgcgat acacgagcgc caagcggtgc cctgtcactc
     4441 gatagcaccg aggtgggaac agccggacac tggacatccc tggaggtggg ggatggttgc
     4501 gccactattg cccgattgct ttctatcgga acaacacgca tactccgaaa gtccattaga
     4561 gtctcacgta agtgctgtcg tgtagtctcg acagggactc ctatggaagt aagattgggt
     4621 tactgctatt gattcttcgg tccatcttca cgcattccta aggttttgaa ttggtattgg
     4681 agtcagcggc aagagtggca cgcttggcat actgtgggcg tgtgcaacag cttgaacttt
     4741 attcgtggtc gcaacaacgg cttcaactaa cttcgtcgga ggatcaaagc gcatgaatag
     4801 gaagatgtgg ggagtttttg caggactgtg cgcactcgcg atgtcggctg tttgcgccgc
     4861 accggccgtc gcgcaggttt cggaaaaacc gccaatgtac tcgtacgtag gagattgggc
     4921 cattcctcgc gcccagtggg cggagatgga aaagtccgcc acggctgacc agagcattct
     4981 cgacaaagcg atcgccagcg gcaccattat tgcctacggc aacgatacca acctggtcca
     5041 ccatcctgag ggcatgacac acgacgattg gtggtcgtcc atgtccatgg ccggtctggt
     5101 caatgttctc gaccagttct acaagtccgg caattcggtt tcgcccgtgt tgtccagcgc
     5161 caccaagcac tccgacgata ttttcgtgag ccggtattac aactaccacg ccggatcgtg
     5221 gaaaggcttg tacacccacg tgtcgacgta caagttgaag gcagacgcgc cggacgacgc
     5281 cgtagaattg ctgagcaaga acatcatcgg cccggtgctg gacaagctgc tcaccgaggg
     5341 tgcgattcac gaatgggaga tcgacaccca agcgatccac actgaggctc cgggcacgtt
     5401 ctgggtcgtt tacattgcgg ccaacgcaga gggtctggac aaggctagcg cagcagtaag
     5461 ggaagccggg aaggcaaacc cgttgcgcgg ccccaccttt aattccgtgg tggactacac
     5521 ggcgcaccgc gatatgctgg cccgcaccaa cgctacatat aaatagattc cggcatttcg
     5581 agcgggcgtc ccactcgagc tcgctgcttg ggtgggattt ctttttggcc tcgaaactga
     5641 atccttcggt tgcagctggt ttgttatggg aacgccggct ggatcggccg ggtgacttac
     5701 tggctgaact ggcggaagat tgaatagctt ggggagggtc gttgttaggt cgtcagcgcc
     5761 gtcaccggag acagacaatt cgccgtgctc tccagttcca gtcctttcgg cagcggctcg
     5821 ccgaatgtac gcaccacgtc ggggcggcct ggcgcgacgt attccaggag tggccgcatt
     5881 agcgcatcgt cggcaatttt ggctcccttg agtttcgcaa ctgccgagcg cgccacgtca
     5941 gcgtcgatgt cgcgagtgcg atcttcgacc cggcggccga gttgcaaaat ggcagacgcc
     6001 gtttcgtgtg taagttcacc caactccagc aacgctgcga tccattccga cgcgctctcc
     6061 gagggcacga cgcaactgag cgatccgtag agcggaattc gcgctccaaa gcggcccagt
     6121 gaccacagcc acccgctgtc ggcgggttcc ttcttcagct tacgtagcaa ctcacttgcg
     6181 agcgcggctc gggtcgcgcc cgacagatgt tcgaggctgg ccagcaatcg ccaaccatca
     6241 tgctcgacct gggaattcag ccgtgtgctg ggtttcttgc ggccgatgcc gaggtctccg
     6301 aggtagttgc ggatctcatg ctggtgggcc gcgccgaggc cgccggcaat gcgccgccca
     6361 agtaccagcc agtcgacctg cgactgaagc tcgcgtggga acaccagtcc cgaagaatag
     6421 attcgccgcg cctgggtgat gcgagcgttg tcctgcgtat ccccgaaccc tgggcgcaga
     6481 caatatccca acaggttgag ccaacgtact tcatgccgcg ggcttagctt tcgtccgtcc
     6541 gccacttcga gcaacgcatc gcagaatagc cgagcggtcg cgagaggcca tgattcccgc
     6601 ttcagatccg taaccgattc caactcgctc accagagcgg caggatcaat tgcatcgccc
     6661 acgcccgctg ccgatttacg gaacgcaacc ttgatgagtt gcacggcctc ttcctgcgat
     6721 tcctgcggca ttaacgctga cagatgcgct gtcgaggttc tctgcgccgc tgacccgagc
     6781 tgatcgtttg cgctggtctc gcgcctcaat tcgaattgca gccgccaacg gtgcgccgaa
     6841 ctcgcggact cgcaccacag ctcgagcgtt cccacctcgg tgaagctgac agacaggcgc
     6901 accgcgagtt ccatctgctg cagcttcttg ccatagcgca gcaaggtgac caggggcgcg
     6961 tgacgatgca cctcgtccgg atccagctcc gcgatttccc cgtgcgcgtc gtggcgaacc
     7021 gtcgagctgt aaagatggaa cgagaccggg cggttggcca gcacggagaa ttcgcggtcg
     7081 agcagcggca aggtcgtgcc ctcattggtg ccggaaggca gcacgcaaac cgccttgatg
     7141 ccttgctccg agcgcattcc gatgtagtag gtgcgagcgc tgccggcctt gacgcgcact
     7201 ccggcacctc gtcgcacgcg cccgtaatag gcggctccga tggcgaccgc gctgctcatg
     7261 acttcgttgc gaagaacctt cggacgccat ccttcgcttg cactgaacca gttggcaatg
     7321 gcgtcggcga tacgctcgcg agcgatggct ggaatgcaga agccgccgtt aaagaggatt
     7381 gagtcgggcc ttaccatcga tgcgcgatca tcgtttcgcg actgagaact ttttccgtcg
     7441 cgcatggcca ttgcggctcg cctgaggaat gccgcaagat gtttcgtgat ggcgggatcg
     7501 ctggcatacg gcaggccgag ctcgcgcagg cctggcgcgc gagctcgcgc aggtatgtca
     7561 tcccgggccg tgagcggcag gaagccgcta gtcagcaact cgactacctc gtcgtgcgtt
     7621 aactcgctgc tcaacatttg accgacgacc gagcgtccgc caccgagaat attgatggga
     7681 aggcggtcga taccttcgtc gccaagcagg cgttctttgg ccgccgaaca tgcgatgcgc
     7741 aaggcatgac gctggcgcaa gctaagcttt gcgttgagct tttgctcgac gcggcgagcc
     7801 gacgcgagat caagattctc gcctcccagc agcagatgct cgccaatggc ggtgcgctca
     7861 aactcgacgc tcgcgccaac aacatgggcg cgcacgaggc tgaagtcgga ggtgccccct
     7921 ccgatatcgc agatgagagc caaatcgccg tcgcgcagtt gctcgctcag cccgttctgc
     7981 tgctccgcca cccacgcgta aaacgcagcc agcggctctt ccagcagagt aaggttcttc
     8041 aggccggctt gccgcgccgc ctccaccgtc agctcgcgag cttcctcgtc gaacgaggcc
     8101 ggcacggtca gcacaatttc ttggttctcg aagagctgct gcggatcagt tacgccatca
     8161 gccgccatcg catggttcca ggcgttccgc agatgcgtaa ggtaacgcgc cgaggcttcg
     8221 acgggagaca ccatcggctc gggcggctca gcctcgcgag gcaaaatgtt ggcagtgcga
     8281 tcgacggcac tctggcacag ccacgacttc gccgaagaca cttggcgtcc cggtatcagg
     8341 gctccctgtt cgcgagccat gacaccgacg atcgaggaag gccgctcttc ccacggcagg
     8401 ttcacggctc ccgatacgat ctcgtctgca gtgggaaagt agaggaacga aggaagtagt
     8461 ggttcggccc gcacttcgcc gggcgccgta agttgcgcga catcgaacgt gtggatgcct
     8521 gacgactcgg ctggtcccgc gccttcagtg gtgtcgacat aagccaccgc cgaatttgtg
     8581 gtgcccaggt cgatgccaat caggtagcgg gacgggccag ccatcacaac ctcgccatca
     8641 cccgccggtt cgtcattggt cgctcttctc gcggatgttc agttcgagct tccagtgatt
     8701 ggcgttgtcg cgcgaaacac accagagttc cagcgtgccg atttcagtga cccgtgactc
     8761 caggcgcacc ggcaacacgg ttccttgctg gccttcgagc gtaagcgtca cttcgagcgg
     8821 gctgagttcc tcgatgtcgc tgccccagtt ttccaacagg tcgcccacat ggtcctgctt
     8881 gcgaatagtc gaactgagga agcggaactc tgcgggctcg ccaactacca ggccgaattc
     8941 acggcccggc acgagtgcct cgctgccttc ctccatcccg aagggcacga cgcaaagcgc
     9001 cttgagcggg gcttccattc cggggacggc gggcatcgcg gtttcgatcc ccacataata
     9061 ggtacgagag gcgccactgc ggatgcgcac tccttgtcca cggcgggcgc gtccgtagta
     9121 ggcggcgccg cgtgccactg catgttcgag gtcaggtgcg tccaaaattt gcgcggcacc
     9181 aagcgcttcg aatccctcag cgaccagcca actgctaagc acctcgacca gacgccctcg
     9241 caatacatct gccttcatga cgccgccatt gaagagaacc tgcgtcgggc acgccaggcc
     9301 actgcgtcct cgccggatgc tggcggcctc cggactgttc tgcacctgct gcgacaaaaa
     9361 gcgcgccaag tgtttggtga cggccgcatc cgcggcatag ggcagaccga gttcctgaaa
     9421 gccaatacgg cgttgccggg ccggcatctc gtcgcttgcc accttgggga aaaagccttc
     9481 gacgagaacg cggttcaagt cttcgcggag caattccgtc ttgatggtac ccccaaccag
     9541 cttgcgacct ttgcccagca aagtgacatc ctgcttctgg ctcttgggat gttcgaacaa
     9601 gtactccttg gcggcgcggc actggtgcca taacgcctgc aactgaaaag tgtcggtctt
     9661 ttgcccttcg gcctccagct tctgttgcag caaccgggcg agagcgaggt ccatgttgtc
     9721 gccacccagc aggatgtgct ctccaaccgc aacgcgcttc agcgccaggt cgccatcttc
     9781 ttcggacaca gcgatgagac tgaagtcggt ggtcccgccg ccgacgtcgc acacaaggac
     9841 caagtccccg accttgatgc gctggcgcca gctatcgccc tggctttcaa gccaagcgta
     9901 gaacgcggcc tgcggttctt cgagcagcgt cacgcgctcg agtcccgcct gctcggccgc
     9961 gcgccgcgtc agctcgcgcg cctcctcgtc aaacgaagca ggcacggtga gcagaacatc
    10021 ttgctctccc aacgtgaggc cttttccgtc ggcggcgtgc tgcgtatccc agaccgtccg
    10081 aaggtactgc aaatattgcg atgagacttc aaccggcgaa agtttgctga cctcgtccgg
    10141 agcctgccac ggcaaaatag gtgaggtgcg attggccgca gcatacgaaa gccaggattt
    10201 tgcggacgcg accagccttc cgggattctc tgcgccgcgc ttgcgcgcca actcgccaat
    10261 cacgaacttc ggatcggcat cccagggcaa gctgaggctg ctcttgggaa aatccagttc
    10321 gcccggaata tagagaaagg atggaagcag cgttcggtca gccacttcat tgggattgac
    10381 cacctgcgga atggcgccga cttcgatgcg cgcgctctcg gcggcggcag cagtatcgca
    10441 gtgggccagc gcggaattgg tggtgcctaa atcgatacca acaatgaatc gtgagtccaa
    10501 ataagtctcc tgtggcgaac catgcgccac gaaaattgct ttgttaagtt gcgaagcagg
    10561 tcccgctaag ggatctcgac ttctgccggg gccacgatcg ctctgcccga accctggggc
    10621 agcgacggca ggtttatctc ggtgacgcgc cagccgcgat gacgcagcag tccgcgaagc
    10681 ggcggttctc ctgcgacgtt ccccaccagc ttgatggccg ccggatcgaa ccccacgggt
    10741 acggtgactg gctgatcttc ctcggaagag agcaccggct caagcttgag gtagcgatcg
    10801 agcacctgac ggcacgaagc gtgaatactg cgcacggccg ccccgagttg gctgtcagga
    10861 tagggagaga cgtcttcggt caggaagtcc accagccggc catcgcgctg gaacaaggtg
    10921 agcacctgga ccgccctgtc cagcgattca ggcgctggct tatcacctaa gctatttact
    10981 gctggtgact tagagacact gagcgtctcc aaaatgtcct gcgggatcat cccctgaaac
    11041 agcagtgaga agaaacagcg aaatgcaaat gatagccggg ttgaaaatcc aatcatagtt
    11101 atgtctgctc cgaagccgag ctggcattac acctaggaaa agccgatgta tcaagggaaa
    11161 cacactgtgg gagggttgta caagctgggg cgtacgggca ttctcgaaat tcgcttagct
    11221 caacagatag aacagtttac gcgttgatcg agcctgaaaa gaagcggcct tgccgccaac
    11281 cagagcaccg aatgcccgta aatttccggc actgcaatcc gaaaagtgca ctacggccga
    11341 cctgtcgata ccaggctttc gcgataccat tcgagtgtac ggcggagtcc ttcttcgagg
    11401 gtaaagcgcg gagcgtgtcc caattccttc acggccgccg tggtgtcggc ctgcgagtcc
    11461 cgcacatcac ctgcgcgcga agcacggtaa agcgtcggaa tctcaacacc ctctatcttt
    11521 tgcagcaagt tccagacctg gttcagagag taccgattgc catttccggc gttgaatacc
    11581 ttgcccgcga ctctgggagc tttcgccgct ttaatgacca ggtcgacgac atcttcgatg
    11641 taggtaaaat cgcgtgtctg ctcgccgtct ccgtaaattg tgggcgaagt acgctccagc
    11701 agactcgtca tgaacaccga aatgacgcct gagtagggac tgccgggatc ctgcctcggt
    11761 ccatagacgt taaagaagcg aagcgaagtt gcgtccagcc catagcagga agaaaaggcg
    11821 ctgcagtaat attcgcccat caacttctgc gcggcgtagg gtgacttggg gttgggcgac
    11881 atagtctcaa ccttgggcag aacttccgta tctccatagg cggacgatga ggcggcataa
    11941 accaccttgc gaactccgcc ctccacacag gcgcgcagga cgttgaaggt cccgtcaaca
    12001 ttggattcgt gcgatggcac gggatcaaca atcgactttg ggacagaggg aatcgccgca
    12061 atgtgaaaca ctgtgtcggc ggcggaaatt accggacgaa tggcatcgaa gttgcaaata
    12121 tcgacttggt ggaagacgat ctgagatgac acctctgcca ggttctgccg gcgtccggta
    12181 gtaaggttgt caagcacctc gaccgatccg tcgcccaacg ctagcagggc gcgaaccaag
    12241 gcggacccga tgaagcccgc tcccccggtt acgacgtatt tcttcatgat ccttcaattt
    12301 ttccttcagg caaatttgag gtttctaatc ttattgtact gtgattaagg cattgagaat
    12361 tgggcgtttg cgagtatggc aggggtgccc ggccaatcgt tgggagttcg atcggcacgc
    12421 aaaccgctcg ctggcagtac gcaagctgag actctggtgc ggaacgctgg agagtgcatt
    12481 gaggccattt ggtgatattc tgtaagttgc catttttgtc gcgcatcggg ccactcataa
    12541 gttagctcct gacgcttgga gttagcttgg aaatgaaaga atgagggatc gatgcgtcgg
    12601 attcgtccgg cagattagga tacccgttcg caccgaaatg cagccgcttc atctggctca
    12661 ccccagactt tgcgcaatcc ggcttccctg ttacgaactg ctgacttttc gtgcggttat
    12721 gcgttaccct atcaatcgct caatgccaca gactaaagac gacttttgcc attgttcaac
    12781 gtcaactaaa cggagaaagt gattaaattg gcgctcgaaa ctacaaactc cactgaaaac
    12841 ccggtggccg tagcgtcaga aaacgccccg gtcgaaggcg gagggggccc caagccgcct
    12901 accctgtggg agcgtattca gacctttggc aggcagcttc agagcttcgg gtggcgcacc
    12961 tggctgctgg ttgccgtgat gattggtctt tatggtccgg tcctggtgcg cctcgtgcgc
    13021 cagtggaaca acgatgccga ctattcgcat gggttcctcg tcccgtttct ttcggcgtac
    13081 ttgatttggc agcggcgcga caaactggca gaagtggatc gacgtccttc caactggggc
    13141 ctgatcgtcg ttgttttttc cctcggactg ctgttcctgg gaagcctcgg cgccgagctt
    13201 tttctcaccc gcatctcgat catcttcacc atctgcggac tcattgttta cttctcggga
    13261 tggcgtttgc tgcgggccct ggcgttcccg ctggcatttc tgctgtttgc cgtaccgatt
    13321 ccaacgctga tctataacga aatcgtattc ccgctccagt tcatagcttc gaagtttgcc
    13381 accagtgttc tggaaatgct caacctgttc cccatcatgc gcgaaggcaa tgtactgatt
    13441 atgcccggaa tgcgcctgga ggtggtggaa gcctgcagcg gcatccgctc gctcatgtct
    13501 ctgctggcat tggcagccgg ttacggatac ctggcggaaa agagcgtgcc cgtccgctgg
    13561 ttcatgttcc tggccatggt cccactggcg atcgtcagca atggtacccg ggtcatgatc
    13621 acggccctga tgaccaatta catcggcccg cgggcggcgg aaggcttcat gcacgagttc
    13681 tcaggctggg tcatattcgt agtggcgacg gccctgttcc tggcgctgca cagcctgacc
    13741 atcgtggtcc gcaaaaagct cggatggatg ccgaaagacg ctgccgtcgc ggcgaaaggc
    13801 caggtaaaag aatgagtaac gcagtgcgat attggacgat gattgccgtg ctactggccg
    13861 ccacggccgg cgtgggcttc ctctcccacg gagagtcaac tccgcccgcc cgtccgctga
    13921 cggactttcc caaggacatc ggcaattaca agacggtcgc cgagatcccg tttgaccagg
    13981 aaacactgac gatcttgggc gtgaccgact atctcaaccg ggtgtacttc tctccaaccc
    14041 agggcgaact cggtttgtat atcggatatt tccgcaccca gcgcacggga gcccagatac
    14101 attcgcccaa gaactgcctc cccggcgcgg gatggcagcc ggtggtttct gaaacctacc
    14161 aacttgcgct ggatgacggc cggaaagttc cgataaacct ttatgtgatc cgtaaagacc
    14221 tcgaccagga aatcgttctg tactggtacc aatcgcacgg ccgcgtggtg gccagcgaat
    14281 attggggcaa gttctatatg gtgtacgacg ccattcgttt gaaccgtacc gacgccgcgc
    14341 tggtgcgcat cacggccccg gtgcgcaacg gcgatctgga cgctgcccgg acccaggcac
    14401 ttgcgttcgc caaacaagct tcagctgatg ttgatcagat tatcccccgg tgacccagga
    14461 gaattcatgc ggaatcgtta tgtcatttcc atagccacgg cgctgctggc cgtcctgctt
    14521 gttggagcaa ccggatgcaa gagcaatccg gaaaaggcca agaaaaagta tctcgagagc
    14581 ggcctgagtt acgtggacaa aaagcagtac gatgctgccg ttatccagtt caaaaaagcc
    14641 ctccaagtcg atccccgata cgccgatgcc cactatcaac tcggactggc ctatctccgt
    14701 cagcagaagg tgaaggaggg atatgccgaa ctccggcaag ctatcgatct cgatcctaag
    14761 aatgtgaagg cacagctgga actcggcaat attctatggt cggtcaagga attcaagcgg
    14821 tcggaagacg ccgcccgcaa ggtcattgaa ctcgacccca acagccccga aggatacacc
    14881 ctgctgggca ctgcactgtt cgcggagaaa gatccggagg gagcactgca ggcctacaac
    14941 aaggcaatcg aactgaaacc gaatgacgcc ggcgtgtact tgaaccgtgg cgtgctctac
    15001 accagcatga aaaaggatgc tgaggccgag gccgatttca agaaggccgt cagtctgaac
    15061 cccaagaacc tcgaggggta ctccaacctg tcgcgtttct atcaatataa gcaggagccg
    15121 gccaaggctg aggaagtgct gcaggaggcc attaagaacg atcccgattc gcccgcgaac
    15181 tacctgcggc tggccggcat gttgctgcag gagaaacgcc tggccgacgc cgaaaccgta
    15241 atcggaaacc tgcgcaatcg gcttcccaag aataccgatg ttgccggtgc gatcgcggaa
    15301 ttctatctgg ccgcgcgcaa ccccgacgct gccatcaagg aatatcaacg tggcctcgcc
    15361 atcgatccca agaatcagaa gttgaccgtc gcgttgggcg acgcctattt gatgtcgggc
    15421 cacgtcgagg aagccaccaa gctcgacgag cagatgctca agaacaagcc caacgatgta
    15481 cccggtcgca tgttgagggg ccgtctggaa gccatccgag gtgacttccc caccgccgtc
    15541 accaccttcc gcggtgtggt aaaggattct cccgaggacc cgcaagccca ttattttctg
    15601 ggccaggccc tgctcaagac gggagacttg aacggtgcca agactgaatt ccaggaagcc
    15661 atcaagcgta atcctgacaa tcccctttat cttcaggccc aggccgaggc ctaccgcgaa
    15721 tcgagagatt tcaccaccgc gaaggaattc gccagccgct tgctgaagct gaaccccaat
    15781 aacccccaga cccacttcct gctcgccagt atcgacctgg gagcaaagga ataccagtcc
    15841 gcgctggaag agctgtcaat cgtagcccag gccgctccga acgaccccac cgtccacctg
    15901 aatatggctt tcgcctacgg gggattgaag aaatatcccg aggcagagcg cgagtttcaa
    15961 accgcgatga agttgaaccc gcagttcgac ggcgccatgg gcgaatacgt cgcgatgctc
    16021 tttgggctta accagggacc gaaggcgctg caggtggcca accaatatgc cactgccaac
    16081 ccaaaccggg cagcagcgca gttcatttat gcctctgccc tggccaacag caagaagctg
    16141 gacgaagcaa ttccagaatt tcagaaggcc attcagctcg acccgaaagc gattttgtcg
    16201 tacatgcagc tggcccgcgt ctacgaaatg cagaacaagc tcgatgatgc gctcgccacc
    16261 ttccagaagg cgcttgcggt tgctcccaac aatgtcgcca tagtgggcgc gatcggcaac
    16321 gtctatctgg ccaggaatga tcctaagacc gcccaacagt acttcgaaaa ggcgaacacc
    16381 ctggcccccc atgatccgta cattcagaac aacctggcgt gggtctatgc tgttcagggc
    16441 cagaacctcg acgtagcgtt gtcgttggcg acgcaagcca aacaggccgc tcccgacgcc
    16501 atcgccatca acgacactct gggttggatc cagtacaaga aggggaatta cgtgatcgca
    16561 gtcgggttgc tgaccgaggt cgtgaacaag ctgccgcaga gtgcggaata ccgctaccat
    16621 ctcggcatgg cgctcaacgc cgccggacag aaggatcgcg cgaaggaaga gctgaaaaaa
    16681 gcgctgcaac tgactccgcc gctcaaccat gacgacaccg gtcaggccca aaccacgctt
    16741 gccaagttgt agccagcgaa atacttccct gttctcaccg cggcctgagg aaactctctt
    16801 caggccgcta atttttggcg gagatcgccc cggcacacat ctggcgcatc ttcgatttgg
    16861 tgtaacccca taaccgcgct tcagatgtga agtttcaata ggttgttgtc ggcccagaaa
    16921 ctttggggat tatcaccttg aaacaaggtg gtcatcggcg caaactctgt gtatccaggg
    16981 attccccgat cacgcggagg tgtggccaag tgaatctaat ttccagcgtg gtgagttgga
    17041 ttgcaattgc aggcgtcttt gtcgttgtgt gggttatctt gcgccgggag cgcattcttt
    17101 ccgcactggc aattgtcgga atcgctggaa ttttactctc gaatcgctat ttgtggcatc
    17161 ttgggcgccc ccatttagac accgtattgc cgattctctc cgacttcgtt cctattgcta
    17221 tggctttggt gggcattatt atgtcctata aacagcctaa gcgcgaaagc cacctatgga
    17281 caaccgtcat acttcttagc gcaggcttca ttggaactgg cattctttcg tggacaagga
    17341 ttaggaacga agcggcgcac aagaaagaag tagagacatt gtctaataaa gtagatgctg
    17401 tcggcactca aaactcttcc atacttggtc aaatagttta attaaaaccc accggagagt
    17461 ctgagcccga ggatattgcg cgtaaacaac acatcctata tcttctacgc aacgagtata
    17521 ttctcggaca cgaggtaact cccggcatct tggcgggcgc tgaatatcct ccagaagcgt
    17581 ggatgaacgc acgactcaag cagttaggac agagttggcg tttcaaggct gagaagcccc
    17641 gcagatacca agaccggagc ctaccagatc atccagactc ctccccctat acaacaagtc
    17701 ataacctcgt caaggagtct cattaatcgc ctgtatgcaa gttggaagca ggcacgaaat
    17761 cgacaacagc gcctagctga aatcggaaga agtaacatct cgttctatga gatcgatcga
    17821 tttgttgaag aatatgacag gggagtacaa gaccgaagta atcgcaacgc gcaaacttct
    17881 tatgggctat attcagggcc agccgccaca gctaagggtg ttggatggac aatttagtgg
    17941 caagcccggc ccgggagaaa ttgagagtca aatcgccgat ttgagagatt tactgaatga
    18001 tgtggagcga gagaaccacc ttgaagaaag ctgtagggac ctaaaactcc ccgtacttcc
    18061 cgagtcagta gaaaggcgca tcaacggatc taggccgcct cagtgagctt cttgttctca
    18121 agatgatccc ttccagcatc ttaagaagca tgtcgcggaa cagaaattgg ttcttccggt
    18181 tgttgaatcg ccggcacact tcatcctgcg cggcaaagaa aaagcgccgc atcctacaat
    18241 gcggcgattc cgattcgtga tgtcccaccc ttctcggcat cgttgccgag agggtggagc
    18301 tagttgttag aagatgatct tccccgccaa ttgcatgttg aacggctcgc ctggaccgat
    18361 accgggtgat ccgttgaagt caccgatggt gtcgctgacg aacccgttcg atccgacgga
    18421 gccggctccc gacgccaagt tgatgcggtt gaagatgtta aacatctcgg cgcgaatctg
    18481 gacagtaagc cgctccttga tggggatgtt cttgatcact gatatgtcca catcggagaa
    18541 gccgggaccg taatacctgt tgcgtgcgag gttcccggcg ccaaccgcag ggacaaaaca
    18601 ggcgggattg acccattgct cgccgcccaa cgctgcactg aagctatggg aaacgccggc
    18661 aaacggatcg cacaccacgt tcattcctgg acgttgtgtg ccagcgttga agttgaacgg
    18721 ctggcccgta tggaagttta cgttacccgc cagctgccac ccgtgagtca acatctcggg
    18781 gcccttcgag gagcctggaa tggtgtacac cagatagccg acgaacagtt gccgcgtatc
    18841 gaagtcgctg ttccccttgt ccagcgcgat gttgaagctg tcgaagggaa tgacaccgcg
    18901 gtattcggtc acctggtcga tggcatgggc ccaagtgtag gagaactgtc cggtcaggcc
    18961 atgccagcta ttcattttca gaactgtctg caaggcattg tagtttgagg tgccgttact
    19021 gttgagttgc aacaccgagc caatgtcggg gtaggcagca ttgaacgcta ggttctggtt
    19081 tatgttcagc atcacgttca gtttgtgggc cgcgcttccc acatacccga attggaatac
    19141 catcttatcg ccaatcgcct tctccaactg cagattgtag ttgtagaagt acggcgtgcg
    19201 gaaattctgg ttcaccgagt acacattaaa ggtctggttg cagccgctca gtgtgggaca
    19261 ggtcgcaacg cccgggaaga tgtattgccc cgccgcccac tggtagttat tgagagagta
    19321 gttggagacc gggttggggc ccacaggatt gtcctgaagt ccgtcggcgc cactgatggg
    19381 aggccggaaa tccaggaagg gattcatgtt gatctggtcg tagaagacgc caaagcttcc
    19441 gcgaaccacc atgtcaccgt tgccatatgg gttccaggca aaaccagccc gtggcgcaaa
    19501 gttattgtgg tccgccggga aaattgagct gatgtcagcg ccctgtatct tgaagccctg
    19561 ccccggtatc cagacggcca ggttcttgtc accgttgtgc agtggtccaa agtattcgta
    19621 gcgcagaccc agcgtgaggt tcagattgcg cgtgacctgc caattgtcct gggtgaaaag
    19681 gctgtagccg tgtgagacca cggcccgctc ggcgtgaccc acggcgatcg aggacgagga
    19741 gacgtagccg gcgagatagt cggcgagggc acatgttggg tctgtggaag cgcaggtgcc
    19801 agcccacggc ccctgggtcc catcaaaggt gaaactgccc agcgaacgac ggtagtaata
    19861 ctcgtccacg tgcccatcgc ggtactctcc gccaaagcgg aactggtgtt tgcccttcga
    19921 ccacgaaagc acgtcggtaa ggtgcccggt aacgtcctgg cgaccttccg gaggtgttat
    19981 gccgacctgg ccaaatcccg aaatcccgat gttagaggcg ccaaagatcg ggttgccgtt
    20041 gatcagcgcg tccgggctca gccacaggcc gagcgccttc atgttgaacg tgttgttggc
    20101 gtcgtggaaa ctctgctgga aatagctgac acctgccagc acctggttgg tcagggtcgg
    20161 cgtcaacacc gagttcaata ccacgttgta gttctgcacg tgcagcggag ccgtctcaaa
    20221 gtaatagccg aggttggagc tcgccgtggc cagcgccagg ctaagtcctg gcggctgtgt
    20281 ctggctgccc tgtccggcga accagtgcgc cgacagcgta ttcttggcgt tgatcgtcca
    20341 gtcaatcttt gccacgccgt tgtagctata gcccgtaccg gcgacggggg agaagtagtt
    20401 gttcaacgta gccggcaggt tggcgaccga actgggccag agagtggtga gcagtgtctg
    20461 ggaaagcgga ttgaccgcca cgccgtattg gttcatcagc gccaccgcct ggtttgccca
    20521 ggcgatcgag ggctcagtcg aaagtccgct cagtcccaac ttataggtct ggtactcgaa
    20581 gcccacaaac cagaacagct tgttcttgat gatggggcca ccaatcactc cgcccacgtt
    20641 ctggttggta agcggagggg ccttgggaaa ttcgggagtg gcaatataga acggcgagtt
    20701 cgccgaatac gcgccgttgc ggttgtagta gtaggcgctg ccatggacct gattggctcc
    20761 gctcttgatc accacgttca cgatgccgcc agcattgcgt ccgttctcgg cattcgattg
    20821 cgtttgcgct gagaagtcct gcaccgcatc aatcggcatg atcacacccg cgattccaga
    20881 gacgccgccc tggttcaccg ccgggatgtt gtgccacatg tcgttgttgt cggtgccatc
    20941 gatctgccag ttcatctggt tggcgcgggt accgttcaga gagccaaatc cgcccactga
    21001 atagccgccg tatccggggg aaactgagat caactgcgaa aaatctcgtc cgttgagtgg
    21061 ggtgtcctgt accgacgtat ccgagatggt catggtttgc gtcgccgagg tagtgtccac
    21121 cgtcagcgct gccgccgata cttccaccgt cgtcgcttcc gctcccatgg agagcttcac
    21181 tggcagggtg taaatcgaac ccgccgtcac cggcaccttg tcgaatgttg ccttgttaaa
    21241 tcccgatgcc gtgaccgtaa cctggtaggt gcccaacggt atgtcctgga aggtaaactc
    21301 tccgccgctg gttgtgaccg tcgcatgctc aacgcccgta gccgtgtccg tggccttcac
    21361 ctgggcgttc gccaccatgc ctcccgtggg atcttccacc gatccgtgaa tagctccgcg
    21421 aaatgtctgc gcagttgccg tcgccgccag cactgcaacg aggatcgaaa ttcttaaccc
    21481 aaacctcatg ccatcaccac ctgatcagat aaagtgaatt gggtgcaccc atctaccgtg
    21541 aggctcgtgc aaaaggcagc attgccgcac accgtgggtg caacttgaat ttttcctaaa
    21601 ccgacagatt cccgatctac aaccccatct acctagtttt taagtggctg acgcttccgt
    21661 gtcaacgcgt ttaaatcaat atgcccgata aacacctttt ataggagtga gttccgtgat
    21721 tctgccactg ccgtggtgca cttcatcaca acatggcgcg aatttgccat atgcgccccg
    21781 gttccggtcc tgaattggct ttgcccctcc ggcgggagga taagtgtaaa ggatgtctcg
    21841 ggacgttaaa cccgagagat ctccaggcat cgcctttcaa cgctggcgac ggatagatag
    21901 ccccctaact ccttatggct gacatggata gatataagtt gttttaaatg aattggttac
    21961 gagcaatttc agcctaactc gatgaaaaca aaggaagggg tgcaaaggaa aaattaagca
    22021 tccaattcga gtttgcgaat cccagcacac cctcgatcct caccgttcct aatcgggaaa
    22081 gaaagcccac tttgtcacag actcccacgg gggaccgaag tatcctcaga aagtagacac
    22141 caagaagtaa gcggcttcgt ctcagaagga gtgaagcatg aaggcttatg tcgtagtgac
    22201 cgcaagggcg ggaaccgcgc gagacattgc tgacgcgatg gccgcactac caggcgtcca
    22261 aatggccgat gcctgctggg gcggaggcga tgtgtacgcg gtagttgact ttcccgactg
    22321 gaaagatcta aacggactgg tattggacaa agtgcactca atgccgggcg tgattcgcac
    22381 cgaaactcac gttgccgtag aacgctagag caaccatcga aacttgggga cattagaatc
    22441 gcagagctgc gctgccccat ctggcgatgt ctcggcgcag gcttcacagt aaggtcagcg
    22501 aatttgcagt ggttaggcgg gagatcgcga tagcagtcgg ctgaccatgt agcccacgat
    22561 catggtggcg cagctaccca tcgcgacata ccacgtgaac ggcacacgcg tgaacagcca
    22621 caggtacacg ttcagcagga agccaaacac cattcccacc atcgctccgc gctccgaggt
    22681 ccgcttggtc aacactccca acagaaacac gccgagcaac gatccataag ctaccgaggc
    22741 gatcgacaac ccaacctcca acaccttgcc accgtgtctc gcgagcatcg ctagcacaaa
    22801 caacactagc gcccacccga tggttgcgaa tcgggacagc cgcactctgt gctcttcggt
    22861 ggactgcggt ctccagcgac cgtagaaatc gacaaccgtg gttgacgaca gcgagttcag
    22921 ggctgcgctc agattcgaca tggccgccgc caggatggcc gagatcagca acccgctaat
    22981 cccgtgtggc atcttcgtca ccacgaatat cgtgaatatc gtgtcaatgc gggtgaatgg
    23041 aaccgccggg ggaaacagtt tgtagaagac gaacaacatc gcgcccacca ccagaaacag
    23101 cgagaactgc gccatcacca caatccaact tgtcatcagg gcgatcttcg actgccgctc
    23161 gctgcgggcc gccagcagcc gttgcacgat gagctggtcg gttccatggc tcgcggtggt
    23221 gagaaatgcg ccgccaatca ggcccgacca aaacgtataa gtggaataga agttccacga
    23281 aaagtcgaat acgcgaaact tgcctgcctc ccctgcgatc gattggacag tgctccagcc
    23341 tcccggcacc agatgaagca gggtgaagaa tccggccacc gtgccgctga tgtagatcgc
    23401 cagttgcacc acgtcagtcc aaatcaccgc ggccatcccg ccctcgaagg tgtagatcag
    23461 ggtgagcagg gtgacgatgg cgatcgccga tacatcgcgc gcgaagtcgc caagtcccgc
    23521 caacagactc gacagtgcaa tgcccacaac gatcgctact gcaaatacgc gcacgccctc
    23581 ggcggcagcg cgcgtgatta gaaacaggcc ggcggtgagc gcgcgcagct tctggccgaa
    23641 gcggcgctcc atgagttgat acgccgtgac caactcgcca cgaaaatatt gcggaatcag
    23701 cacgaggcag atgaggaagc gcccagccag gtagccaatc actaattgca ggaaacggaa
    23761 gtccttctca taggcaagcc ccggtacgct gatgatggtc agggtgctgg tttcggccgc
    23821 cacaatagag agcgagatcg cccaccacgc gatttgcttg tccgcgagga agtagtcttt
    23881 cagcgttcgt tggcgcttgc gaaagcgcag accaaacgcg gtcaccccgg tcaggtaaac
    23941 ggcgatgacg gaaagatcga tggcattcag tcccatcctc tatcgctttc ctgggttgtc
    24001 cagacttgcg aaccgggagg ggccgcaatt tcgctcagca ccatggtttt taacttccgc
    24061 cgctcggcag gtactttatg gactgtatac ggtcatgccc aagggtttgc gcgtcgcttc
    24121 ggcgttaaaa tcacgacgtg ccggcaacca ccggatgact cgggtggcag tgtaccagca
    24181 cgctgaacgc gcctgccttt tgcgtcaaga acagcatttt gtcggagttg ggtttccgcg
    24241 cgaaatatgc agaattattg ggatgttcag agattttcag agaaagcagg agcgggctta
    24301 gctgatgagc ttactggcgg agagggggga ttcaaaaaac acgaaaattg gcaagtcgga
    24361 acacatcaac aagttagcca ccaactttcc tatttacaat cacttactga cttttagcgc
    24421 agtggaactt aacttccgca gtttctcctt ttggctcaaa ttccaccaaa aatggtatca
    24481 ctggtatcac ctgcgcagtg gcggagaggg tggagagggg gcgttggagc ggttcagaaa
    24541 aatacaaaaa tcacaggcgc cctcgtagaa cacgagatgg tatcacgaga tggtatcacc
    24601 tccgagttcc actgctaccg tcggagaggg ttgcaaggca agttgacttg caatggaagc
    24661 aggagacgtt cgtgtgtcgg cgtgggaagt tgggagagag cgtgaagatt ccgttcagtg
    24721 cattcgagag cagccattcg gctgcgggga gagccaatcc agccaagccc caagttgaag
    24781 gcaacca
//
LOCUS       DASRKK010000119        13760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_496103, whole genome shotgun sequence.
ACCESSION   DASRKK010000119 DASRKK010000000
VERSION     DASRKK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496103"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFC15_02735"
     CDS             complement(<1..470)
                     /locus_tag="VFC15_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09108.1"
                     /translation="MKWVAGVVVWLAVLGTATSLAAQVLTPTELSDPKTQRLQQKYFK
                     ALVAIGDQVGAHKFPYPFYFSRVLDVDQSKMQMQDQRSIRFDFYKNQTVLEMTGNYYA
                     AYSADRMDSYARLKETFQQVVMPMLQAAVPLFPDDSAFSAFAIEVSHHVREKALG"
     gene            complement(494..1222)
                     /locus_tag="VFC15_02740"
     CDS             complement(494..1222)
                     /locus_tag="VFC15_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM09109.1"
                     /translation="MNRALIVLSALTLLTSGCSHRSKQPAPLKATATGAAIVESSGGK
                     QIGYIGMPLDQPVVVQVNDASGSAVAGAVVYFGGPSGVSFTPAAALTDSSGQVTTNVS
                     LGQQAGRYKLVASTRDATGKNIELPIEEIALGYQQTVGAQLNAQYCIRCHDPESSSLR
                     VSNMDNLSVKPHAFTEGDTLNKMSDADLIAIISHGGPALNKSAEMPPWGYTLSKSDIA
                     ALVSYIRAVADPPYEAGGLVYAQK"
     gene            complement(1219..3264)
                     /locus_tag="VFC15_02745"
     CDS             complement(1219..3264)
                     /locus_tag="VFC15_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM09110.1"
                     /translation="MADKLKSLWRFFFGDSARAFGSMSLILLVLLAISPAKDHFSQWR
                     HYQKQYLHLIRGRADAVTLERHYEGGIHQIWIPEEQVTDRCTTCHLGLKENSLADVSA
                     VAYRKHPVIPHSLTEFGCTTCHRGQGVATTVQEAHNATEQWEQPLLPAKYIEAGCGQC
                     HLEKQTATPFLNQGRQLISSYGCTHCHTIKQGDGTYIQPDDDPPSLKHVADKTTREWV
                     YAWIKDPQAYASTATMPNYHFGDDDARDVSAFILAQSTPLEPSLQPLNAKPNPDPAAG
                     TSLYGQSFCASCHAMQNAAGMMVGGNVGPELTGIGTKVKPEWLADWVTNPDHYDPATM
                     MPHYRFDATQVATLAAFLESKTEPDFVASVHLEAPTPQQIEHGKRLVLENGCASCHEI
                     NGIKKPENFAPELNRIGSKPISQLLFVEGVDHNLPSYVAAKVRNPRSFGPALKMPQFQ
                     LTPQQVDAVTTALLAQTDRAATQPPSMRIATVQQSKYEPAGHAGQLMHDLRCFSCHMI
                     NGHGGDMAPDLTWEGTAVTREWLTQFLKNPETLRPALIRRMPKFNLSDADINTLTDYI
                     MTVYQTPEFDRDALPLTEFTPAMVEQGRELFYSKFACQACHIADYAKDKGYIGPALAG
                     TGRRFNAAWVYHWLKDPQALRSGTIEPDQHMTDDEARSLTAFLMSLKSTGAKEVAKK"
     gene            complement(3271..3975)
                     /locus_tag="VFC15_02750"
     CDS             complement(3271..3975)
                     /locus_tag="VFC15_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09111.1"
                     /translation="MSDPKDFVAVVDSDARRTPRRIAFITKRSSPQVHVKDEDRVMTY
                     PEVFFRAAVAIEVMTAALVIVALLWDAPLESLADPMHTPNPAKAPWYFLGLQEMLHYF
                     PPVVAGVLVPGLVVMALIVIPYFKINVEAEGLWLRDRARRLRIFGIVVVLFCIFLVVF
                     DVWVALVPTLIVASFMLLAAQTNPETAQGFRKWLVRKPLSFWVMTWFLVELVVLTVVG
                     TLFRGPGWSWVLPWRA"
     gene            complement(3972..4727)
                     /locus_tag="VFC15_02755"
     CDS             complement(3972..4727)
                     /locus_tag="VFC15_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZM09112.1"
                     /translation="MANGVDNLVNQVRENRIWRSIFRSGSGESNLRRALFVQQNVFLH
                     LFSIKSRKRVLEFSATWYLGALTFGTFLILVITGILLMLYYHPSVPQAYTDMKDLQYV
                     VSSGVFLRNLHRWSAHAMVFLVFAHMFKVFYRGAYRTPREFNWIIGVVLLLVTLLLSY
                     TGYLLPWDQLAYWAVTVGSNIASAVPLVGAKMRFLMLGGTAVNANALLRFYVLHCVIL
                     PVTAVLFIAVHFWRIRKDGGIYPGESASEVSSK"
     gene            complement(4730..5224)
                     /locus_tag="VFC15_02760"
     CDS             complement(4730..5224)
                     /locus_tag="VFC15_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZM09113.1"
                     /translation="MAEDPKRALAPDSPEEMSRRQFFVKVGVGSAAVAALGTAGFAYQ
                     YLSPNVLYEPSPIVDAGKPDRYAPDSVTMDPQTAIYVVRTGEGFFALSAICTHLGCLT
                     AYKPDLGIIACPCHGSKFNKDGVKIEGPAPRPLPWLRMWLSDDGNLMVDRSNAIAIKQ
                     LVRV"
     gene            complement(5242..7209)
                     /locus_tag="VFC15_02765"
     CDS             complement(5242..7209)
                     /locus_tag="VFC15_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM09114.1"
                     /translation="MLSRFTRKTVDREWLNTNFPCMMACPAHTNAGRYVTLIAEGKFE
                     EAYKYARDPNPMASICGRVCAHPCETACRRGEIDRPIAIRALKRFLTENYGPESRHPV
                     APTTRPAAKLPYKVAVIGGGPVGLSAAHDLALMGYSVTIFEASPVAGGMLYLGIPEYR
                     LPRSVVEAQVREILETGDISLRLNCEAGRDFTVGELRHQGFDAVLIGVGAHRSRDLSI
                     PGVDLDGVHRGIEFLLNVNLGYKFTIGKKVLVIGGGNVAMDVARSAAREVLRQHDAPS
                     EITPSEENAQAVAAHEMMDISLSALRMGAREVGLVCLENRDEMPAALEEIEEAETEGI
                     IMHPGFGPKRVLGRDGKVVGLECLKTKYVFDENHRFSPAFYDNSDTQFECDTIILSIG
                     QAPNLDFLRPEDGVLVSPRGLINVHRDTLMTSAPGIFAGGDCAFGPRLIIDSVGDGKR
                     AAVGIDEYLRGTKHQEPKIEVQVLDRWQMAVDYMNIQRQAIPMLPLERRTGMTEVELV
                     YTQQEAMAEAQRCLKCYVNTILEGSEQDGTDCVLCGACVDVCPENCLELVPLDRIEFE
                     PNAIDALANNPELFTFELEGIQPAELGSIAGTAMIKDETRCIRCGLCALRCPANTITM
                     EAYHLVPSEPTGLITIQSFDGPRPVAAAAAK"
     gene            complement(7290..7871)
                     /locus_tag="VFC15_02770"
     CDS             complement(7290..7871)
                     /locus_tag="VFC15_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM09115.1"
                     /translation="MTRRFTLFTILLVLILATAAFAGTITGTVSPGKSSVVYVDAIAG
                     KTFPAPAAKPLMDQKGLMFNPHLLVVQVGTVVEFQNSDNVQHNVFWPSISGNKKDGHN
                     LGTWPKGEKRPFTFSTPGVVPLLCNVHPEMSGYIVVSPTPYFAETDASGNYKIENVPD
                     GKYNVVAWHEGMKPQTKSVDVAGTGKADFTLSK"
     gene            complement(7912..9462)
                     /locus_tag="VFC15_02775"
     CDS             complement(7912..9462)
                     /locus_tag="VFC15_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09116.1"
                     /translation="MYGTSCSTCHIDFPKLNDFGKAFKDAGFKFPKDDESMLKIPPVM
                     LGAPANEELWPKAIWPGTIPGIPPIGMRYNNYLQFTSGSRNNFNNLLPPGSIGPYVPT
                     ADFETGLFSIFTAGNFGSDIAFWVDDDISVSGANANGGLGEAYLKFVNVGRFLKLPEY
                     ALNLRVGQVELDIPFTQSKSIWVSPYDIYSQANVGVVNPAFNQQFVNNSFTFAGSGQG
                     IEFSGGRHTGGYNYSLAFINQNTSGVGQDSNPGSPYIPQATGSNNGGLGVASDANFKD
                     IYASFNYRFNLEKDKESRNAIQAAGPSGPRDHTYLNFGTYYFYGRSVQRLTGALLDGT
                     AATLTAREPFYRVGGNFTFNYHCCLQFNGLFMYGHDYNLQPIDSTGALIPLPVDPTVS
                     TPVAFVHGSPTTFSGGFVDAEWLAYPWMYVMIRYDAVNSQSDYANALVPGGFTNSSFN
                     GAASATRNRITPAVQFLIHANIKAVAEYQFRPGQSVTYAMNPITGLPMAVNSFHTNTL
                     VLGFEFAY"
     gene            10246..11319
                     /locus_tag="VFC15_02780"
     CDS             10246..11319
                     /locus_tag="VFC15_02780"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZM09117.1"
                     /translation="MKKKLAITAVVSLTVLMVSMQLFSAAHSSAVRNRIPAPSPSPAD
                     VIAGPGRIEPVSEDIKLSSELSGKLKSVDVEEGDPVRMGQVLAVLENDDYRAQLASAT
                     AQVQEKEAILRKVTNGARSEERSEAQAAVSQAEAVMANAQADLARRQSLYQSGIISRE
                     ELERYTREYKVAKGEYEEKPNRYSLLNTASREEDVAFAQADLRLALANEAESQARYDK
                     TIIKSPIDGIVLRKHHRNGESVSNSAASPDPVLTIGDASVLRVRVDVDESDVNKVRVG
                     QSAYVRADAYGNQKISGHVVQVGRLLGPKTIRTDEPTERVDRKFMETVVELEPGAQLP
                     VGLRVDAFILTDGVQAAAVRPQM"
     gene            complement(11907..12698)
                     /locus_tag="VFC15_02785"
     CDS             complement(11907..12698)
                     /locus_tag="VFC15_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZM09118.1"
                     /translation="MSSSSFGKHASGATSGQSAVPEPSFAERARTLMHSGRIGTLSTL
                     SWKQPGFPFGSLMPYGLDRQGRSIFLISTMAMHTQNLQADPRASLFVTESGASGDPLG
                     SSRVTLIGNVVRIPEPELAAARAGYLSGYPDSKYWVDFEDFFFYRMDILDVYYVGGFG
                     VMGWVAAMDYSQAQPDPLTEHKHDIMQHINADHKDALILLSKRFAGIEAQDAEMTSVD
                     RLGFNVRLKAQDGVHGARIAFLRKVTNPTETREVFVEMVKQARQE"
     gene            12800..13105
                     /locus_tag="VFC15_02790"
     CDS             12800..13105
                     /locus_tag="VFC15_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZM09119.1"
                     /translation="MKFQQFSYGQIRIDGIEYGYDVVIDRGEIRERKKKPSKRFRDSF
                     GHKPLSLEEEIPWKCSRLVVGTGTGALPVMDEVRREARRRNVELIILPTEEAMELLN"
     gene            complement(13206..>13760)
                     /locus_tag="VFC15_02795"
     CDS             complement(13206..>13760)
                     /locus_tag="VFC15_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09120.1"
                     /translation="RLPIVTRPLAGDVKRALEKTLPDPKMEYKLVRRQAGLGSLGQQR
                     FVAIADWQGGRIAREAKAVLPSACTWLEGKLGHGQSYYQQAISSAIRSHDPYQEIVGS
                     WLIRRLSPDSNPIDIQDLPKRPDEQMLLHAMGSEAANVHLGTKRQVHNILQDLQNRKP
                     NWLRDAAKRMARAVEKEWKEFRHG"
BASE COUNT         2907 a         3940 c         4016 g         2897 t
ORIGIN      
        1 cccaatgcct tctcccgaac atgatgcgac acctcgatgg cgaaggcgga gaacgcggaa
       61 tcgtcgggaa aaagcggcac ggccgcttgc agcatcggca tcaccacctg ctggaaggtt
      121 tccttcaagc gggcgtacga gtccatgcga tcggcagaat atgcggcgta gtagttgccg
      181 gtcatttcca gcacggtctg gttcttgtag aaatcgaagc ggatggagcg ctggtcctgc
      241 atctgcatct tcgactgatc gacatcaaga acgcggctga agtagaacgg gtacgggaat
      301 ttgtgcgcgc ctacctgatc cccgattgcg accagcgcct tgaagtactt ttgctgcagc
      361 cgctgcgttt ttgggtcgga cagctccgtc ggcgtgagaa cttgcgccgc cagggacgtc
      421 gccgttccca gcaccgccag ccacaccacc acgccagcaa cccacttcat cccggcatct
      481 tctccgtcgc gcactacttt tgagcgtaca ccaatccacc ggcttcgtaa ggcggatcgg
      541 cgacggcgcg aatgtaagac accagtgcgg ctatgtcgct cttggacagt gtgtaacccc
      601 agggcggcat ctctgcggat ttgttcaagg cggggccgcc gtggctgatg atagcaatca
      661 agtccgcgtc gctcatcttg ttcagggtgt cgccttcggt gaaggcgtgc ggtttcaccg
      721 acaggttgtc catgttcgac acgcgcagcg acgaagattc cggatcgtgg caccgaatgc
      781 agtactgcgc attgagttgc gctcccaccg tctgctggta gcccaaagcg atttcctcaa
      841 tcggaagttc gatgttcttc ccggtcgcat ccctggtgct ggcgacgagt ttgtaacgac
      901 cggcttgctg tccaagcgag acgttggttg ttacctgccc actggagtcg gtcagggccg
      961 cggccggagt aaaactcacg ccggatggcc cgccaaaata aacgactgct ccggcgactg
     1021 cgcttccgga ggcgtcgttc acctgaacga caaccggctg atccagtggc attccgatat
     1081 agccaatctg cttgccaccg ctggactcaa cgatggccgc gcccgtggcg gtggccttaa
     1141 gcggagcggg ctgtttgctc ctgtgcgagc atccactggt gagcagcgtc aaggcgctaa
     1201 gaacgatgag cgcgcggttc atttcttcgc cacctccttc gcgccagttg acttgagcga
     1261 catcaggaat gcggtcagcg agcgcgcctc atcgtcggtc atgtgctggt caggctcgat
     1321 cgtgccggac cgcagcgctt ggggatcttt cagccagtga taaacccacg ctgcgttgaa
     1381 gcgcctcccg gtgccggcca gggccggacc aatgtagcct ttgtcctttg cgtaatcggc
     1441 gatgtggcac gcctggcagg caaatttcga atagaagagt tcccgccctt gttcgaccat
     1501 ggcaggtgtg aattctgtta gcggcagagc gtcgcgatca aactccggcg tctggtacac
     1561 agtcatgatg tagtcggtga gcgtgttgat gtccgcatcg ctgaggttga acttcggcat
     1621 acggcggatc agcgctggac gcagggtttc cggattcttc aggaactgcg tcagccactc
     1681 ccgcgtcacc gccgttccct cccaggtaag gtcaggagcc atatctccgc cgtgaccatt
     1741 gatcatgtgg caactgaagc agcgcaggtc atgcatgagc tgccccgcat gtccggcggg
     1801 ctcgtatttc gattgctgga ctgtcgcgat gcgcatcgaa ggaggctggg tcgccgcgcg
     1861 gtccgtctgc gccaaaagcg cggtggtgac cgcatccact tgctgcggcg ttagctggaa
     1921 ctggggcatt ttcaaagctg gcccgaaaga ccgcgggttg cgtactttcg cggcaacata
     1981 cgagggcaga ttgtgatcga cgccttcgac aaagagcagt tgcgagatcg gcttgctgcc
     2041 gatgcggttc aactccggcg cgaagttttc cggcttcttg atgccgttga tctcgtggca
     2101 actggcacag ccgttctcga ggacgagccg tttgccgtgc tcaatttgtt gcggagtcgg
     2161 agcctcgagg tggacgctgg caacgaagtc gggctccgtc ttcgactcca ggaaggcagc
     2221 cagcgtcgcc acttgagttg catcgaagcg gtaatgcggc atcatggtgg cgggatcgta
     2281 gtggtccggg ttcgtcaccc agtcggcgag ccattcgggt ttgactttcg ttccgatgcc
     2341 tgtgagttcg ggtccgacgt tgcctccgac catcatgccg gcggcgttct gcattgcgtg
     2401 gcatgacgcg cagaaggact gaccgtagag cgaggtcccg gcagcgggat cgggattcgg
     2461 cttggcgttg agcggttgca gcgaaggctc cagaggcgtg ctctgcgcca ggataaacgc
     2521 cgagacatcc cgcgcgtcgt catcgccgaa gtgatagttc ggcatggttg ccgtgctggc
     2581 gtaggcctgc gggtctttga tccaagcgta aacccattcg cgcgtcgtct tgtcggcgac
     2641 atgtttcagc gatggcggat cgtcgtcggg ttggatgtat gttccatctc cctgtttgat
     2701 ggtgtggcag tgtgtgcaac cgtagctgga tataagctgc cgcccctggt tcaggaacgg
     2761 tgtcgcagtc tgcttctcca gatggcattg tccgcaaccg gcctcgatgt atttcgccgg
     2821 cagcaggggc tgctcccatt gttcggtggc gttgtgcgct tcctgcacgg tggtagcgac
     2881 accctgtcca cgatggcaag tggtgcagcc gaactccgtc agtgagtggg gaatgaccgg
     2941 gtgtttgcgg taggccaccg cactcacgtc ggcaaggcta ttttctttca gcccaagatg
     3001 gcacgtcgtg cagcgatcgg tcacctgctc ttctgggatc cagatctggt gaatgccacc
     3061 ttcgtaatgg cgctccaggg taacggcatc agcgcggcca cggatgagat gtaagtattg
     3121 cttctggtaa tgccgccact ggctgaaatg atctttggcc ggggaaatag cgagcagcac
     3181 aagcagaatg agactcattg agccgaaagc ccgtgcactg tccccgaaga agaaccgcca
     3241 cagtgacttg agtttgtcag ccatcgtcgc ttaggccctc cagggcagga cccaggacca
     3301 tcccgggccg cggaagagtg ttccaacaac ggtcagcacc acaagctcaa ccaggaacca
     3361 ggtcatgacc cagaaagaca gcggctttcg caccagccac tttcggaagc cttgtgcagt
     3421 ttcagggttg gtttgcgcgg caagcagcat gaatgacgca acaattaatg tgggtaccag
     3481 ggcaacccag acgtcgaaga ccaccagaaa aatacagaac agcacaacca caattccgaa
     3541 gattcgcaag cgccttgctc tgtcgcgcag ccacaaccct tcggcttcca cattgatctt
     3601 gaaatacggg atgacgatca gagccatcac cactaatccg ggtacaagta cacccgccac
     3661 cacgggcggg aaataatgca gcatctcctg caggccgaga aagtaccacg gggccttcgc
     3721 tggattcggc gtatgcatgg gatcggcaag gctctcgagc ggcgcgtccc atagcagcgc
     3781 gacaatgacc aaggctgcgg tcatcacctc gatggcaacg gcggcgcgga agaagacttc
     3841 gggataggtc atgacccggt cttcatcctt cacgtggacc tgcggcgaac tgcgcttggt
     3901 gatgaacgcg atccggcgcg gggtccggcg cgcatcggag tcgactacag cgacgaagtc
     3961 ttttgggtcg ctcatttgct gctcacctcc gaagccgatt cgcccgggta aatgccaccg
     4021 tctttgcgga tccgccagaa gtgcaccgca ataaagagaa ccgcagtcac cggcaggatg
     4081 acgcaatgca gaacgtagaa ccgtagcagc gcattggcgt tcacggccgt tcctcccagc
     4141 atcaggaatc gcatcttcgc accgaccagc gggactgccg acgcaatgtt gcttcccaca
     4201 gtaaccgccc agtaagccaa ctggtcccac ggcagcaggt agccggtgta gctcaggagc
     4261 agcgtcacca ggagcagcac tacaccaatg atccagttga actcacgcgg cgtgcggtag
     4321 gctccgcgat agaagacttt gaacatgtgc gcgaaaacca ggaagaccat cgcgtgtgcc
     4381 gaccagcggt gcaggttacg caagaacacg ccggacgaaa ccacgtattg caggtctttc
     4441 atgtccgtgt aggcctgggg gaccgaggga tggtagtaga gcatcagcag aatgcccgtg
     4501 attacgagga tgaggaaggt tccgaatgtc agcgcgccta gataccatgt cgcgctgaac
     4561 tccagcacgc gtttgcgtga cttgatcgag aaaaggtgca gaaacacgtt ttgctgcacg
     4621 aagagcgcgc gacgcagatt gctctccccg ctgccgctgc ggaaaatgga ccgccagatt
     4681 cggttctcgc gcacctggtt tacgagattg tcgacaccgt ttgccatggc tacaccctca
     4741 ccagttgctt gatcgcgatg gcattggagc gatcgaccat gaggttgcca tcatcgctca
     4801 gccacatgcg caaccacggg agcggcctcg gcgccggtcc ttcgatcttc acgccatcct
     4861 tgttgaactt gctgccgtgg cagggacagg caatgatgcc cagatccggc ttataggccg
     4921 taagacaacc cagatgagtg cagatcgccg acagcgcgaa gaaaccctcg ccggttcgaa
     4981 ccacgtagat cgctgtctgc ggatccatcg tcaccgagtc aggagcatag cggtcgggct
     5041 tgcccgcatc cacgatcggc gacggttcat acagcacgtt gggcgacagg tattggtacg
     5101 cgaaaccggc tgtgcccagg gccgctactg ccgccgagcc gactccgact tttacgaaga
     5161 actgccgccg gctcatttcc tccgggctat cgggagcaag cgcgcgtttt ggatcctcag
     5221 ccataaatcc tcactgaatg attacttggc cgctgccgca gcgaccggtc tgggtccatc
     5281 aaagctctga atggtgatga gtcccgtggg ttccgacggc acaaggtgat aagcctccat
     5341 ggtgatggtg ttcgcggggc agcgcagggc gcacaagccg cagcgaatgc agcgcgtctc
     5401 gtccttgatc atcgcagttc cggcgatgct gcctaactcc gctggttgta tgccttccaa
     5461 ttcgaaggta aataattcag gattattggc cagtgcatcg atcgcgtttg gttcgaactc
     5521 gatgcgatcc agtggcacca gttccaggca attctccgga cacacatcga cacacgcgcc
     5581 gcagagcacg caatccgtgc cgtcctgctc gctgccttca agaatggtgt tgacgtagca
     5641 cttgaggcat cgctgtgcct ccgccatggc ctcctgctgg gtgtagacga gctcgacctc
     5701 ggtcatgccg gtgcggcgtt ccagcggaag catcggaatg gcctggcgct ggatattcat
     5761 gtagtccacg gccatctgcc agcggtcgag cacctgcact tcgatctttg gctcctgatg
     5821 tttcgttccc cgaaggtatt cgtcgatgcc caccgccgcg cgcttgccat cgcccacgct
     5881 gtcaatgatg aggcgcgggc cgaaggcaca gtcgccgcct gcgaagatcc cgggggcaga
     5941 ggtcatcagg gtgtcgcgat gcacattaat cagcccgcgg ggcgacacca acacgccatc
     6001 ttctggtcgt aggaaatcca gattaggcgc ctgcccgatc gaaagaatga tggtgtcgca
     6061 ctcaaactgg gtatcactgt tgtcgtagaa cgcgggacta aaccggtggt tctcatcgaa
     6121 gacgtatttg gtcttcagac attcgagacc gacgaccttg ccgtcgcgtc ccagcactcg
     6181 cttcggcccg aaaccgggat gcatgatgat gccttctgtc tccgcttcct cgatttcttc
     6241 caaagcggcc ggcatttcat cgcggttctc caggcagacg agacccactt cgcgagcgcc
     6301 catacgtaac gcagacagcg aaatatccat catttcgtga gcagcaaccg cttgcgcatt
     6361 ctcttccgac ggagtgatct cgctcggagc gtcatgctga cgcaatactt cacgcgcggc
     6421 ggagcgggcg acgtccatgg caacgttgcc tccgccgatg accagcacct tcttaccaat
     6481 ggtgaacttg tagccgagat tgacgttgag caggaactca atgccgcgat gcacaccatc
     6541 caggtcaacg cctggaatcg acagatcgcg actgcggtgt gcgcctacgc cgatcagcac
     6601 ggcgtcaaag ccctggtgtc gtagctcacc aaccgtgaag tcacgccccg cttcacagtt
     6661 cagacgcaac gatatgtcgc cggtttcgag gatctcgcgc acctgcgcct caaccacgct
     6721 gcgcggcagc cgatactcag gaatcccgag gtacaacatt ccgcccgcga cgggagaggc
     6781 ctcgaagatc gtgaccgagt agcccatcaa agccagatcg tgcgcggccg agagcccgac
     6841 cggccctccg ccgattaccg caactttgta cgggagtttt gccgctgggc gggtggtcgg
     6901 ggcaaccggg tggcgcgact ccggaccgta attctcggtc aggaaacgct tgagagcgcg
     6961 aatcgcgatg ggccgatcga tctcgccgcg cctacacgcc gtctcgcagg gatgcgcgca
     7021 aacacgcccg cagatactcg ccatcggatt gggatcgcgc gcatacttat acgcttcctc
     7081 gaatttccct tccgcgatca gcgtgacgta acggccggcg ttggtgtgcg cggggcacgc
     7141 catcatgcag ggaaagttgg tgttcagcca ttcccggtca accgtcttcc tggtgaagcg
     7201 gcttagcatc agccctctct ccgacgaggt gtggttgttt gtcagtgcaa gaggttgggc
     7261 gcacctgccg gggcgccctt cctaaaccgc tatttcgaga gggtgaagtc cgctttgccg
     7321 gtgcccgcca catccactga cttagtctgt ggcttcatgc cttcgtgcca tgcgacgacg
     7381 ttgtacttgc catcgggaac gttctcgatc ttgtagttcc cgctggcatc ggtctcggcg
     7441 aagtagggag tggggctgac aacgatgtag cccgacatct ccgggtgaac gttgcacagc
     7501 agcggcacca ctccaggagt actgaacgtg aatggacgct tttcgccctt cggccaagtg
     7561 ccgaggttgt gtccgtcctt cttgttgccg ctgatcgaag gccaaaacac gttgtgctgc
     7621 acgttgtcac tgttctggaa ttcaaccacc gtccctacct gtacgaccag gaggtgcggg
     7681 ttgaacatca gacctttctg gtccattaac ggcttcgccg caggggccgg gaaggtcttg
     7741 ccagcgatgg cgtcaacgta aacgacggag gacttccccg gagatactgt gcctgtgata
     7801 gtgccggcga acgcggcggt tgcaagaata agaaccagca gaatcgtgaa cagcgtaaaa
     7861 cgacgtgtca tatatcttcc tttcataagg tctaacttta ttgaacactt attagtaggc
     7921 aaactcaaat cccaaaacca gcgtgttggt atggaacgag ttcaccgcca tcggcaggcc
     7981 ggtgatcgga ttcatggcat acgtgaccga ttgccccggc ctgaattggt attcggcgac
     8041 cgccttgatg ttggcgtgaa tcaggaattg cacggccggc gtaatgcgat tgcgggtcgc
     8101 gctggctgcg ccgttaaagg atgagttggt gaatccccca ggcaccaggg catttgcata
     8161 atctgattgg gaattcaccg catcgtaccg aatcattaca tacatccacg gatacgccag
     8221 ccattcagca tccacaaatc cgccgctgaa agtcgttgga gagccgtgca cgaacgccac
     8281 cggcgtcgag actgttgggt ccaccggcaa cggaatcaaa gcgccggttg aatcaatcgg
     8341 ctgcaggtta tagtcgtggc cgtacataaa caggccgtta aactggagac agcagtgata
     8401 gttgaaggtg aagttgccgc ctacgcggta gaacggctcg cgggcagtca acgtggccgc
     8461 ggtgccgtca agcagggcgc ccgtgaggcg ttgcactgag cgtccgtaga aatagtacgt
     8521 cccaaagttg agataggtat ggtcgcgcgg cccgctgggg ccggccgcct gaatggcgtt
     8581 gcggctctcc ttatcttttt ccaggttgaa gcgatagttg aagctggcat agatatcctt
     8641 gaagttggca tccgaagcca cgcccagtcc gccgttgttg ctgcccgttg cctgcggaat
     8701 gtacggacta cccgggttcg agtcctgtcc cacaccgctg gtattctggt tgatgaaggc
     8761 aagcgaataa ttgtagccgc cggtgtgtct gccgccgctg aactcaatac cctgaccgga
     8821 tcccgcgaaa gtgaacgaat tgttgacgaa ctgctggtta aaagctgggt taaccacgcc
     8881 aacgttggct tggctgtaga tgtcatacgg actgacccag atggacttcg actgggtgaa
     8941 cgggatgtcc aactcgacct ggcccacgcg caaattcagc gcgtactcag gcagtttcag
     9001 gaaccggccg acgttgacga acttcagata agcctcgccc agtccgccgt tggcgttcgc
     9061 ccctgacacg ctgatgtcgt catcgaccca gaacgcgatg tcgctgccaa agttgccggc
     9121 agtgaagatg ctgaagaggc cggtttcaaa gtcagcggtc ggaacgtagg ggccaatcga
     9181 ccctggcggc aataggttgt tgaaattgtt gcggctgccg ctggtgaact gaaggtaatt
     9241 gttgtagcgc attccgatcg gcgggatgcc cggaatagtg cccggccaga tcgcttttgg
     9301 ccataattct tcattcgccg gcgctcccag catgaccgga ggaatcttca gcatcgactc
     9361 atcgtccttg gggaacttaa aacccgcatc cttaaaggct ttgccgaagt cgttcagctt
     9421 gggaaagtcg atgtgacagg tgctgcaact ggtgccatac atccgtgaaa atgcgggtat
     9481 gccaaaactc tggcgggatg aaagtaccaa tagaattaat aagatagact gaacgggtac
     9541 gctacaacga aggaacggtt tcacgcgcat atcgacccct tctgtggact ccgaaatcca
     9601 ggccagatga agtaccgtgt cgcccacttc tgatttggcg aattggaacg agagtattta
     9661 cgtttcgcca acccctcagc agtgacgtac atcacgctcg acggtgatgt tagtactact
     9721 gccattattg tgaccctgat ctgtcgagca ctacgactgg cccatcatcg cactcgagca
     9781 ccgataccca cggcaactgc cgcgggctga cgtgcgggga tgcgcgagcg tgcggagtag
     9841 attggcgcaa agctgcaagt cctgagccgg gcagattccg gaaccgaggt cgagcttcgt
     9901 tttcgggcaa agtcgccttc gaatcctcca cgccgcggcc atggtttatg tggtcggacc
     9961 gattctttgt cgccaagcca gcggtcaacc gtccatcgac caaatagaac ggtccccagc
    10021 agttttatct tccgagaata cctacatagt ggtagctgct aagtcctcca gcgcagtagc
    10081 tcatggttgc ccactggcgt tagcgaaaaa gcctcttatc tcgtgatgtg aatgtcggct
    10141 aaacaccgca tagtaccagc atgcaaggcc ggttcggatc agccagatcc ggggactacg
    10201 caccacggcg tttgcgatag aaccaatctt tcgatggagg atgcagtgaa aaagaaactg
    10261 gcaattacag ccgtggtcag cctgacggtc ttgatggtct caatgcagtt gttttccgcc
    10321 gcccatagca gcgcagtccg taaccgtatt cccgcaccgt ctcccagccc cgcagatgtt
    10381 atcgccggac caggacggat cgagccagtc tccgaagaca tcaagttgag ctcggagctg
    10441 agcgggaaat tgaagtcagt ggacgtagag gagggcgacc ctgtacgcat ggggcaggtc
    10501 ctggcggtgc tggagaacga cgactaccgt gcgcagttgg cttcggctac cgcgcaggtg
    10561 caggaaaagg aagccatctt gcgcaaagtc accaatggag cgcggagtga ggaacgttcc
    10621 gaagcgcagg cagcagttag tcaggcagaa gcagttatgg ccaatgccca ggcagatttg
    10681 gcccgacgcc agtccttgta tcaaagcggc atcatctcac gcgaagaact ggagcgctac
    10741 acccgcgaat acaaagttgc caagggtgaa tacgaggaga agcccaaccg ctattccctg
    10801 ttgaatactg catctcgcga agaagatgtt gccttcgcac aagccgattt gcgcctggct
    10861 ctggcgaacg aggccgaatc gcaggcacgg tacgacaaga caatcattaa gtccccgatc
    10921 gatggaattg tgctgcggaa gcaccaccga aatggcgaga gcgtttccaa ctcggccgcc
    10981 agtcctgatc cggtgctgac gatcggcgac gccagcgtac tgcgcgtacg ggttgatgtc
    11041 gatgaatccg acgtaaacaa ggtccgcgtg ggtcaaagtg cctacgtaag ggcagatgcc
    11101 tatggcaatc agaagatctc gggacatgtc gtacaggtcg gcagactact tggccccaag
    11161 acgatccgta ccgacgagcc aaccgaacga gtggaccgta agttcatgga gactgtggtc
    11221 gaactcgaac ccggggctca gttgcctgtg gggctccgcg tagacgcctt catcctgact
    11281 gacggtgttc aggcagcggc ggtgcggccg cagatgtagc aatttcacat taaacctaca
    11341 cgaagagtgt catcacagaa agcagccaca tgacagcaac taccatgaca accaccaaca
    11401 gagactctag tcggcacaat gttcgcgggc tcatgagcta cggtcagaaa tggcttgtga
    11461 ctcttgtttt gacaatggcg gtccagtgtc cacccgtggc aattgccatc atgctttacc
    11521 acacattgaa gtactaccgg atctagtctt cgtttgttgg cacaaacggc gcagcttccc
    11581 catcaccgtg cgcggcaaac cgtgccgctg tactgcggca gtgtcggtat ccggaacata
    11641 catggtttgt ctcgattgct gtcgcgaatt cccctacgac tggttgcaga tgaagatgct
    11701 cgcggcaaaa tcccgcgatg tgcgcgcgag cccgtcacca acgttcccgg tctcaaggcc
    11761 gcttgaggtc tgggcttggc cgttcgcgat gatcaatgac cttcgcgtcc agtccggcga
    11821 acaacgtgct ttttcctggg tgggcgatag atgtcccgct ccctaataaa tgcaggtctc
    11881 aacgatgagg agaaaaccga tgagcactac tcttgacgcg cctgcttcac catctcgacg
    11941 aacacttctc tggtctccgt cggattcgtg acttttcgca ggaaagcgat gcgcgctcca
    12001 tgcacgccat cttgggcttt caggcggacg ttaaagccga ggcgatcgac cgaggtcatc
    12061 tccgcgtcct gtgcctcaat gccagcaaac cgttttgaga gcaggataag cgcatctttg
    12121 tggtcggcat ttatgtgctg catgatgtcg tgcttgtgct cagtcagcgg atcgggttgc
    12181 gcctgggagt agtccatggc agccacccat cccatcactc cgaagccgcc cacgtaataa
    12241 acgtccaata tatccattcg gtaaaagaag aagtcctcga agtcgaccca atacttgcta
    12301 tctgggtagc cactcaaata gccggcacgt gcggcggcca gttctggctc gggtattctg
    12361 acgacgttcc ctattaacgt gactctggat gaaccgagcg ggtcaccgct ggccccggac
    12421 tctgtcacaa acaggctggc gcgagggtca gcttgtagat tctgcgtatg catggccatt
    12481 gtgcttatga ggaagatgga gcggccctgc ctgtccagcc cgtaaggcat cagagacccg
    12541 aaaggaaaac ccggctgttt ccacgacagg gtggagagag tgccgatgcg gccggagtgc
    12601 atcaacgtgc gcgctctctc ggcgaacgaa ggctcgggaa cggcagactg gccacttgtg
    12661 gcgccgctcg cgtgttttcc gaaggacgag gaagacattg cttaacaccc tctcggtatc
    12721 agcataacct tcaactttcc atcacggagt actggcaggc cacgcagcgc ctcgattgta
    12781 tccttatcag aggctcgcta tgaagtttca gcagttctcg tatgggcaaa ttcggatcga
    12841 tggcattgag tacggctatg acgtcgtgat cgaccgaggg gagattcgcg agcgcaagaa
    12901 gaaaccatcg aagcggttcc gggacagttt cggtcacaag ccactctctt tggaagagga
    12961 gataccgtgg aagtgcagtc ggttggtggt gggcacgggc actggagcac tcccagtcat
    13021 ggatgaggtc agacgtgagg cacggcgacg caacgttgaa ttgatcatcc tccctacgga
    13081 agaagccatg gaactcttga attagcgcgc cgacaacacc aaagccattc tccatctgac
    13141 ctgctgagcg ttagaatccg ctgcagcggc gctgggcatg cggctagagg tcgaagacct
    13201 gatcgtcaac cgtggcggaa ttctttccat tctttttcca ctgcgcgcgc cattcgtttt
    13261 gctgcatctc gcagccaatt cggttttctg ttttgcaggt cttgcaaaat gttgtgaacc
    13321 tggcgtttgg tgcctagatg gacattggca gcttcagatc ccatcgcgtg tagaagcatt
    13381 tgctcgtctg gccgctttgg caaatcttga atgtcgatgg gattagaatc cggcgaaagt
    13441 cggcggatca accaagaacc tacgatctcc tgatagggat catgggatct tatcgccgaa
    13501 gagatcgctt gttggtaata cgactgccca tgtcccagct tgccctccag ccatgtgcaa
    13561 gcagaaggta ggactgcctt ggcctctcga gcaatgcggc ctccttgcca atcggcaatc
    13621 gccacaaatc tttgttggcc aagactgcct agcccggctt gccgacgcac caacttgtat
    13681 tccatcttcg ggtcgggcaa ggtcttctcc agcgccctct tcacatcgcc cgcgagtggt
    13741 cttgtgacaa taggcaagcg
//
LOCUS       DASRKK010000120         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1375694, whole genome shotgun sequence.
ACCESSION   DASRKK010000120 DASRKK010000000
VERSION     DASRKK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375694"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1636)
                     /locus_tag="VFC15_02800"
     CDS             complement(374..1636)
                     /locus_tag="VFC15_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZM09121.1"
                     /translation="MSNQFSGATRALPDRPNLRHLKDQAKDLVKAGDARSLSDAQLQI
                     AREYGFSSWPKLKAHLDSLEEAGQLKDAIDRNDLPLVQSMMLRNRGLHKAPLGYAKDG
                     PLTWVAECRAPGVRPNQTRLAMAKWMIETGSDVHQGGDAPLMRAALAGERIPMMELLV
                     AHGADVNGGWHGDYPIIFAACEALSPDTLRWLLEHGADPNCGSEAVWKARGIPHPGTA
                     LDYLLGAYVRDPEVLGACIEILLRAGGKSKHESPAVLAVIRGRMTELTELLDASPALI
                     HQRFPGLDFGTTAARMLTLRGGTLLHVAAEFQSLDAARLLLDRGADVNAAATLDETGV
                     GGQTPIFHAATQREDAGLPLVQLLVERGADLTVRARLPGHYEVPGEVVECTPLDYALL
                     FPGDQGPTSAYLKGRTDSGWASALIAPE"
     gene            complement(2061..>2517)
                     /locus_tag="VFC15_02805"
     CDS             complement(2061..>2517)
                     /locus_tag="VFC15_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09122.1"
                     /translation="YFDNGDDRRNPSIWKVPANGGHAMQLARGTVTFPLESPDGQYVY
                     FARYQKLWRVKTDGSSEEPVAGMPDFNFLGDEWVPFGSGIYFLSHEGSKTVINLFDLQ
                     TKHVRPIFTLEKPTPAWLGGMVVSSDGKWLLYSQVDQASCDLMLIEDWR"
BASE COUNT          511 a          765 c          720 g          521 t
ORIGIN      
        1 gctgaacggc cggcggcagt tgctgccgcg cccgctgcaa ggaataagaa ctcgtcccac
       61 gcaggttcgg gaaggtggag gactctcttc gacggaggcc cgaaggctcg ttctgggaag
      121 tcacttggac gtgttgtcgg cgactcggtg aagtggccgc cagccgagca cccccgataa
      181 gccggccagt gttcacgaaa atgcccacct gctccggatc actggcggca actttcgccc
      241 tttggaccgt ctcctcaccc aaatggagcg agttctctaa atcaacttgt cgcggcaagt
      301 gaccaaagcc gttgtggaag cgcgcgtgag agcttggtca tcgggcagtc ttgaggagac
      361 ttttacgatg ccgtcattca ggcgctatta gggccgacgc ccaaccagag tccgtacgtc
      421 ccttcaggta agcggacgtt ggtccctggt ccccaggaaa tagcagcgcg taatcaagcg
      481 gcgtgcactc caccacttcc cctgggacct catagtgccc tgggagtctt gcacggaccg
      541 taagatcggc gcctcgctcc accaacaatt ggacgagcgg aagcccagcg tcctctcgct
      601 gtgtcgccgc atggaatatg ggtgtttggc cgccaactcc ggtttcgtcc agcgtcgctg
      661 ctgcattgac gtcagcgccg cgatccaaca atagccgcgc agcatcgagg ctttggaact
      721 cggctgccac gtgaagcagc gttccgcctc gcaaggtgag catgcgggct gccgtcgtac
      781 caaaatcaag tccgggaaat cgttgatgga tcagagcggg acttgcgtcc agcagttcgg
      841 tcagttcggt catgcgacca cggatcaccg ccagtaccgc gggcgactca tgcttggatt
      901 ttccgccggc gcgaagtagg atttcaatgc aggcgccgag cacttcagga tcccggacgt
      961 aggcacccag caaatagtcg agggccgtcc ctggatgcgg aatgcctcgt gccttccata
     1021 ccgcctcgct tccacaattg ggatccgcgc catgctccaa cagccagcgc aaggtgtcgg
     1081 ggctgagggc ctcgcaggcg gcaaagataa taggatagtc gccgtgccaa cctccgttga
     1141 cgtccgcgcc atgggccacg agcagttcca tcatcggaat tcgctcgccg gcgagtgcgg
     1201 cgcgcatcag gggtgcatcg cctccctgat ggacgtccga cccggtctcg atcatccatt
     1261 tcgccatagc caaacgagtc tggttcggtc gtacaccagg cgctctacac tcggcgaccc
     1321 aggtaagagg tccatccttg gcatagccca gcggagcctt atgcaaccct cgatttcgca
     1381 gcatcatgga ttggactagg ggcaaatcgt ttcggtcgat cgcatccttg agctgtccag
     1441 cttcttcaag cgaatcgaga tgcgctttca gtttgggcca gctcgagaac ccgtattcac
     1501 gagcgatttg aagctgtgca tcggagagag agcgagcgtc gcccgccttc actaggtcct
     1561 tggcctgatc cttcaggtga cggaggttcg gacgatcagg aagggcgcgc gtggcgccag
     1621 agaattggtt cgacattgcg aactcctttc atcgaagtgc ccgccgtccg caatttcggg
     1681 ctgaaagaag tttcgcgaag ccaggttgac ttgcaggtgg gcttctaccc tttccgcgga
     1741 ccggaagcgt ccttcacgcc ttctacaagc ctactacggc agtaacggaa accacttgac
     1801 gtatagtcgg catctcgcgg aagtgtgacg gacccgcact ttctatgcgc cacatccgcg
     1861 cgtagggcgt tttacggcca agtgcggtgc caatgcgatt gccggccgac ttatcgttcc
     1921 taatcgaaag aaagggacgg gtcccgagcg gctgcgcgtt tccttacggc tgcctcaact
     1981 cctgcgaagg catgttcccg tcaactgcca gccgacttcc gtatggctcg tcaaccaggt
     2041 ccaatccgga tgagcgtccg ttagcgccag tcctcgatca gcatcaggtc gcaagaggcc
     2101 tgatccacct gcgagtagag cagccactta ccatcactcg acacgaccat cccaccaagc
     2161 caggctggcg taggtttttc cagcgtgaag atcggtcgga cgtgcttggt ctggagatcg
     2221 aacaggttga tcaccgtctt gctgccttcg tgggatagaa agtagatgcc cgatccaaat
     2281 ggaacccatt catcccccag gaagttgaaa tcaggcatgc ctgccaccgg ctcttcactt
     2341 gatccgtcag tcttgacacg ccaaagcttc tgataccgag caaaatacac gtactgccca
     2401 tcaggagact ccagcggaaa ggttacggtc cctctggcca gttgcatggc gtgtcctcca
     2461 ttcgcgggta ctttccaaat gctggggttt ctgcgatcgt ccccgttatc aaaatag
//
LOCUS       DASRKK010000121         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_593822, whole genome shotgun sequence.
ACCESSION   DASRKK010000121 DASRKK010000000
VERSION     DASRKK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593822"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFC15_02810"
     CDS             <1..213
                     /locus_tag="VFC15_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09123.1"
                     /translation="QENSMPQSPHDRLAELHNLAAHAHAAAATAHGKGDHLTAHELSE
                     QAHQHSMNAYKHAKKLMTVATQSKKP"
     gene            262..1506
                     /locus_tag="VFC15_02815"
     CDS             262..1506
                     /locus_tag="VFC15_02815"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZM09124.1"
                     /translation="MNVFVLNSGSSSLKFQIIATDLDRIKQHQDERVCRGEVERIGGE
                     AIVTFQKGADAKQKFTAPLRDISAALEYLIRYIASDKSGVAEIKSSGDMHAVGHRVVH
                     GGEMFSESALIDAKVLQDIEDCIDLAPLHNPNNIKGIVAARELFGKDVPQVAVFDTSF
                     HHSIPEQAFLYALPYHLYRRHRIRRYGFHGTSHRYVAFRYRALRGLTREQTNIITLHL
                     GNGCSATAIRRGNSVDTSMGMTPLEGLVMGTRSGDLDPAIVNLIATKEGMSPSEVDTL
                     LNTQSGLLGISGLTNDMRMLQAELQENHDRRVQLAIEVFCYRARKYIGAFLAAMGGAD
                     AVIFTGGIGENSPEIRARICTGMEWAGLEIDAAKNQQTIAREGLISTDDSRLLAYAIP
                     TDEELLIARDTVRVILGEPHPS"
     gene            1648..>2895
                     /locus_tag="VFC15_02820"
     CDS             1648..>2895
                     /locus_tag="VFC15_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZM09125.1"
                     /translation="MGRPTTSVEGFWDRPLQELFELLPATPAGLTTEEARRRLRLYGP
                     NSLVHESRFAALLSFFGFFRNPLVVILVIASGVSLALGEHVGGLIILAIVLFSVLLNF
                     LMEFQARHAVEDIQKQIALTAAVVRDGREQELPTADLVPGDIVRLNAGDLVPADARLL
                     EVRDLHVRESVLTGESLPVEKTVADLPKEKHGVADASNSVFLGTAVQTGMGTAVIVCT
                     GKDTACGEIANRLAMRPPETEFDRGIRRFGMMLTWVTMLLVLFVLLVNIVFHRPVLES
                     FLFSVALAVGMTPEMMPMIITVTLAQGARRMTKKKVLVKQLSAIEDFGSVDILCTDKT
                     GTLTEGEIVLDRHVDFQGKDNENVLQLIYLNSHFEAGIKSPLDDAILKHEAPSIVGYD
                     KVDEIPFDFNRKRLSVVVRHADEE"
BASE COUNT          634 a          827 c          839 g          595 t
ORIGIN      
        1 caggagaatt ccatgccaca gagcccgcac gatcgactcg cagaacttca caacctggca
       61 gctcacgccc atgctgccgc cgccaccgcc catggcaagg gagatcatct gaccgctcac
      121 gagctgagcg aacaggcgca ccagcactcc atgaacgcgt acaaacacgc caaaaagctc
      181 atgacggttg ccacccagtc caaaaaacca taggaccccg ggacttgctg gtacgataca
      241 tcccaaaaga gaaagcccgg catgaacgtc tttgtactca attccggaag ttccagcctg
      301 aagtttcaga tcatcgcaac cgatctggat cgtatcaagc agcatcagga tgaacgcgtc
      361 tgccgcggcg aagtagaacg catcggtggg gaagcgattg tcacattcca gaagggcgcc
      421 gacgcaaaac agaagttcac cgctcctctg cgagacattt cggcggcgct ggaatacttg
      481 attcgttaca tcgcctccga caaatcggga gtcgctgaga tcaagagttc gggtgacatg
      541 cacgcggttg ggcatcgcgt tgtacacggc ggcgagatgt tctcggagtc ggcccttatc
      601 gacgccaagg tattacagga tattgaagac tgcatagacc ttgcgccact tcacaatccg
      661 aacaacatca agggcatcgt ggctgcgcgc gaactcttcg gcaaggatgt tccgcaagtg
      721 gccgtatttg atacttcgtt tcatcattcg atccccgagc aagctttcct gtacgccctc
      781 ccctatcacc tgtatcggcg tcaccgcatc cgccgatacg gcttccatgg aacctcgcat
      841 cgttacgttg cgtttcgcta ccgcgcgttg cgcggactga cgcgggagca gaccaacatc
      901 atcacccttc atctcgggaa cggctgctca gcaaccgcaa ttcgccgagg aaattcggtg
      961 gatacctcca tgggcatgac acctctggag ggtctggtga tgggcacgcg ctcgggagat
     1021 cttgatccag cgatcgtgaa tctcatagcg acgaaagaag gaatgtcgcc gtccgaggtg
     1081 gatacgctgc tgaatacgca atcgggtttg ctgggtattt ccgggctgac aaacgacatg
     1141 cggatgttgc aggccgagct acaggaaaac cacgatcgtc gcgtacagct cgcgattgaa
     1201 gtcttttgct atcgcgcacg gaagtacatc ggtgcattcc tcgccgccat gggtggcgct
     1261 gacgcggtga tcttcaccgg cggtattgga gaaaactcgc cggagatccg cgcccgtatt
     1321 tgcacaggca tggagtgggc cggccttgag atcgatgccg ctaaaaacca gcagacgatt
     1381 gcccgcgaag gcctgatcag caccgatgat tccaggctac tcgcctatgc cattcccacc
     1441 gatgaggaat tgcttatcgc ccgcgacacc gtccgggtca tactcggcga gccgcacccc
     1501 tcctgatgcg cttcgactat gcagggggcc gtccctggct ctgtgatagg cgggttcaac
     1561 ggcggtcgca ctacccgccg atggccggga aatacattag tgagcaacgc tctcatggga
     1621 taatgcggca gttacgggag ggcccggatg ggaagaccaa caacctcggt cgagggcttc
     1681 tgggatcggc cgctgcagga actgttcgaa ctgctaccgg caaccccggc agggctgacg
     1741 accgaagagg cgcgacggcg attgcgtctt tacggcccca acagcctggt ccatgagtct
     1801 cgcttcgctg cgctgctcag tttttttggt ttcttccgca atcctctcgt cgtcatcctt
     1861 gtcattgcca gcggcgtatc tcttgcactg ggagagcacg tcggcgggct gatcatcctt
     1921 gcgatcgtac tgttcagcgt actcctgaat ttcctgatgg aattccaggc ccggcacgcc
     1981 gtggaggaca ttcagaaaca gattgcactg accgctgcgg tggtgcggga tggacgagaa
     2041 caggaactgc cgactgccga tctggttccg ggagatattg tcaggctcaa cgcaggagac
     2101 ctggtgccag ccgacgcccg attgctggaa gtgagggacc tgcatgtgcg cgagtcagtg
     2161 ctcactgggg aatctttgcc ggtagagaag acggtggctg accttcccaa ggagaagcac
     2221 ggcgttgcgg acgcgagcaa cagcgttttc ctgggcactg cagttcagac gggaatgggc
     2281 accgcggtca tcgtctgcac gggaaaagat accgcctgtg gtgagattgc gaatcgtctg
     2341 gctatgcggc caccggagac ggagttcgac cgcggcatac gccgcttcgg aatgatgctc
     2401 acatgggtga ccatgttgct tgtccttttc gtcctgctgg tgaacatcgt tttccatcgt
     2461 ccggtgctgg agtctttcct tttctcagtc gcgcttgccg tcgggatgac acctgaaatg
     2521 atgcccatga tcatcaccgt gacgctggcc caaggcgcga ggcggatgac gaagaagaag
     2581 gtcctggtca agcaactgtc agcaatcgag gatttcggca gcgtggatat tctctgtacc
     2641 gacaagacgg gcacactcac cgaaggtgag atcgtgcttg accggcacgt ggatttccag
     2701 ggtaaggata acgagaacgt cctgcaactc atttatctga acagccactt tgaggcgggg
     2761 atcaaaagcc cccttgacga cgcaatcttg aagcacgagg cgccttccat tgtgggatat
     2821 gacaaggtcg acgagattcc gttcgacttc aatcgcaagc gcctttccgt ggtggtgcga
     2881 cacgcagacg aggaa
//
LOCUS       DASRKK010000122         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_887177, whole genome shotgun sequence.
ACCESSION   DASRKK010000122 DASRKK010000000
VERSION     DASRKK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887177"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFC15_02825"
     CDS             complement(<1..633)
                     /locus_tag="VFC15_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09126.1"
                     /translation="MANPGQHSRLIFAWNLRKTTFALAVVCGLTMAALQSAQAQVFTT
                     LHTFTGGGDGATPYAGLSMDRAGHLYGTASSGGHRGGSCGNGCGTVFKMEHKGSSWLL
                     VPLYAFSGPDGATPQARVIIGPNGNLYGTTTYGGSAGKGVVFRLQPPPAACKTALCPW
                     METVLYSFAGGSDGAYPTFGDLTFDRQGNIYGTTPDGGSGSGCQGGCGVVY"
     gene            complement(828..1205)
                     /locus_tag="VFC15_02830"
     CDS             complement(828..1205)
                     /locus_tag="VFC15_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09127.1"
                     /translation="MRTLDIIIGWLLIAFGVVHISLTRRVHPDIDINAIWFASGGLLM
                     ITIGALNLLRVAYSSVAKGVYVVSVIANIVLLLLMLFIATRVPMRSNPQVLVGLILAA
                     LLTAFSVLRRADHIQARQMQSAK"
     gene            complement(1508..2791)
                     /locus_tag="VFC15_02835"
     CDS             complement(1508..2791)
                     /locus_tag="VFC15_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09128.1"
                     /translation="MPLLARPQLVLSLVCPIVLSVCLITPSQAQTFTVLHNFTGGADG
                     YDPSAGLTIDRAGHLYGTTLFGGYTGGTCAGFGCGTVFRLTQQGSGWLFTPLYSFQGA
                     GDGSVPSAGVVFGPNGRLYGATQYGGNDQCSAPYGCGTIFDLAPPAHACGRALCAWTE
                     TVDYRFNGGISAESPTGNLIFDGAGNIYATAAGGGLPNCYAGTTCGVVYELSPSRGGW
                     GETDLYSFTAGNDGYLPASGVIRDQAGNLYGTALYGGDNGCGTVFQLTPSGSGWSETT
                     LRDLQSQTDGCGPNELIADPQGNLYGTDRRNGPNGGGTVFELTKLSGGGFSFRVLYAF
                     SGMPGGPMAGLTMDSAGNLYGTTYQNGAHNYGSVFKLTKAGSGWTYSDLYDFTGGSDG
                     AYVMSTVTIDANGNLYGTASEGGTYANGVIWKITP"
     gene            complement(3041..>3619)
                     /locus_tag="VFC15_02840"
     CDS             complement(3041..>3619)
                     /locus_tag="VFC15_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09129.1"
                     /translation="TFEGGFVSPPDNNGYGVIFELTPSGSGWTESVVNKFFDGNDGGL
                     PAASLTMDQAGNFYGTTYAGGTGACISGYYTGCGSVFLNDGQPIYTFGNLQQFAPLSG
                     PLASVSLDASGNLYGTTSGDGINSLGNVFRLTSRQYAYTSLHDFTGRSDGGAPVSSVV
                     VDATGNLYGTASIGGTGSGCPGGCGVIWEITP"
BASE COUNT          805 a         1041 c         1025 g          748 t
ORIGIN      
        1 atacacgacg ccacagccgc cctggcagcc ggaaccgctg ccgccgtccg gggtggtgcc
       61 gtagatgttg ccctgtctgt cgaaggtcaa gtcgccaaac gtgggatacg cgccatcgct
      121 gcctccagcg aaggagtaca aaacagtttc catccacggg cagagcgcag tcttgcaggc
      181 agcaggcgga ggctgtagcc gaaagacgac gcctttgcct gcggagcctc catatgtggt
      241 ggtgccatag agattgccgt tggggccgat gatgacccgg gcttgcggag tggctccgtc
      301 gggcccgctg aacgcataga gcggaaccag aagccagctt gaacccttgt gctccatctt
      361 gaagacggtg ccgcatccgt taccgcagga acctcctcgg tgccccccag acgaggcagt
      421 gccgtagagg tgcccagctc gatccatgct caagccggcg tacggagtcg ctccgtctcc
      481 tccaccagtg aatgtgtgca gtgtcgtgaa aacctgcgcc tgcgctgatt gcaatgcagc
      541 cattgtgagc ccacaaacca cagcgagcgc gaaggttgtt ttccgcaggt tccatgcgaa
      601 gatcaatctg gaatgttgtc ccgggtttgc catgatggtc tcccttactg aatatgaccg
      661 ttgacgctcg ccgttttcga ggttggcatt ttacacccgg acgttgcaaa acttccacgg
      721 gaaaacgata gctgttgtaa tcaggcgatt acactcaaaa cccctgtccc ggtcaatgag
      781 tgtaacgggg atattacgcc aatcatttcc cgccgcgctg acaagctcta ttttgcactt
      841 tgcatctgac gagcttgaat gtggtcagcg cggcgaagca ctgagaatgc ggtgagcagt
      901 gccgcgagaa tcaaaccaac caagacctgc ggattgctcc gcatcggaac ccgggttgcg
      961 ataaacagca tcaggagcaa tagcacgatg ttcgcgatca ccgataccac gtagacaccc
     1021 tttgcgacgg agctatacgc cacccggagt aggttgagcg ccccgattgt gatcatcagc
     1081 aggccgccac ttgcgaacca aatagcgttg atgtcgatgt cggggtgcac cctccgcgtc
     1141 aacgatatgt gaacgacgcc gaacgcgatc agtagccagc caatgataat gtcgagtgtt
     1201 ctcaagcttc cctccagaac ttatgtgttg ttcctcaact ctaaagcaga tacagcatag
     1261 tcgggaatgg ctggcgagca cagacgttgt cataagcgat ccgccgctgc cggattactt
     1321 acgatatggc gatttcccat tagctattcc gagggtctca ccgcaggcac gtcaggcatt
     1381 gcttctgagt ggccgacgaa tcgagcggct atcgcgtcgt agcaagaaaa gtccgacacc
     1441 ccaaatcctg gatgtcaggc cgcaaatcat ttcagcccag gtcgctatgc gtttgcggag
     1501 tttcagttta cggcgtgatc ttccagatga caccattggc gtaggtgccg ccctctgacg
     1561 cagtgccata gagattgccg ttggcatcga tggttacggt actcatgacg tatgctccat
     1621 cgctgccgcc cgtaaagtcg tagagatcgc tgtaagtcca accgctgccc gccttagtca
     1681 gcttgaacac tgagccgtag ttgtgcgctc cgttttgata ggtcgtgccg taaagattgc
     1741 cggctgagtc catggtgagg cccgccatgg gcccgcccgg cattccggaa aacgcatata
     1801 gcactctgaa ggaaaagccg ccgccagaaa gtttcgtcag ttcaaaaacc gtgccgccgc
     1861 cattgggtcc gttcctccgg tctgtaccgt agagattgcc ctgcggatcg gcgattaatt
     1921 cattcgggcc gcatccgtcg gtctggcttt gaagatcgcg tagagtcgtc tccgaccagc
     1981 ccgaacccga gggggtcaac tggaagaccg tgccgcaacc gttgtcgccg ccatagagag
     2041 cggtaccgta gagattgcct gcctgatcgc ggatgacacc gctcgccggg aggtagccgt
     2101 cgtttcccgc ggtaaagctg tacaggtcgg tctcacccca accgccccgg gatggtgaca
     2161 gctcgtagac gacaccgcag gtagttcccg cataacagtt aggcaaaccg ccccccgcag
     2221 cggtggcgta gatattgccc gctccgtcga atatgagatt accggtggga ctttcagcgc
     2281 tgatgccgcc attgaaacga tagtccacag tttccgtcca ggcgcacagc gccctaccgc
     2341 aagcatgggc tggaggagcc agatcgaaaa tagtcccgca gccgtaaggt gccgagcatt
     2401 gatcgttccc gccatactgg gtcgcaccat agagcctccc attcggaccg aagacgacgc
     2461 cagccgaagg aaccgacccg tcgcccgctc cctgaaagct atatagagga gtgaagagcc
     2521 aaccggaacc ttgttgggtc aacctgaaga cggtgccgca cccaaatccg gcgcacgtac
     2581 caccggtgta gcctccaaaa agagttgtgc cgtacaggtg ccccgccctg tcaatggtca
     2641 atccagcaga aggatcatat ccgtccgctc caccggtaaa gttgtgaagc actgtaaagg
     2701 tctgcgcctg actcggtgta atcaggcaga ccgagaggac aattggacac actagggaca
     2761 gaactaactg aggacgcgct agcagtggca tggcagattc cttgttcgtt gtgacattgg
     2821 ctgggagcga ttgtttcgtg accggttgta gattctggtc ttaggagtag gtcgctgtca
     2881 atccgtttgt cgagtaggcg acagtcgagg tcgcgacgac gttgacaact gttgcaaaca
     2941 ggcgattaca cgaccaactc tgataacagc acctaagtgc ggattgcttg tgcaatggcg
     3001 atgttacgac actttccagc aaaacacttc ggtatatcaa ctagggcgtg atctcccaga
     3061 ttacgccgca gccgcctggg cagcccgagc ctgtgccgcc aatcgaggcc gtgccgtaga
     3121 ggttgccggt ggcatcgact actacgctgc ttaccggagc accaccgtca ctgcggccgg
     3181 tgaagtcgtg gagcgaggtg tatgcgtact gccgtgacgt cagtctgaag acattgccga
     3241 ggctgttgat gccgtcacct gaagttgtgc cgtagaggtt gcccgatgcg tccaagctga
     3301 cagatgccaa gggtccagaa agcggagcaa actgttggag gttgccaaaa gtgtaaattg
     3361 gctgtccatc gtttagaaag acggagccgc aaccggtgta gtatccggat atacaagctc
     3421 cagtccctcc ggcataagtc gtcccataga agtttcccgc ctgatccatg gtcaggctcg
     3481 ccgccggaag gcccccgtca ttaccgtcaa aaaacttgtt gacgacgctc tctgtccaac
     3541 cggagcccga gggtgttaac tcgaagatca ctccgtatcc gttattgtcc ggaggcgaca
     3601 cgaagcctcc ctcaaaggt
//
LOCUS       DASRKK010000123         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1180505, whole genome shotgun sequence.
ACCESSION   DASRKK010000123 DASRKK010000000
VERSION     DASRKK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180505"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..876)
                     /locus_tag="VFC15_02845"
     CDS             complement(427..876)
                     /locus_tag="VFC15_02845"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HZM09130.1"
                     /translation="MSTSISQSNPTLDRLEDQISWYDNKSRVNQRWYKWLKLLTMVSA
                     VFVPVLSVPNWGRHYVAALGIIIVLAEGIQQLNQFQANWIAYRSTCESLRHEKYLYLA
                     QAGPYQSTDKPITVLAERIEGLVSQEHAKWVSAQNEAGKKTSQATAP"
     gene            complement(935..1489)
                     /locus_tag="VFC15_02850"
     CDS             complement(935..1489)
                     /locus_tag="VFC15_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09131.1"
                     /translation="MERRYLVAALAIIATFAVFSRGFRTLEHVSLSYGQHVGAAAKAK
                     CNADASAASRFMGKVRTHLRPGYPEEAQLLAEMNLPIAMAQAKVAEQVARQNLAAAQC
                     ARATALREAERARREATRMRDKMAHTHGDASAMPIAWGVNAAADVDQRVRAKTAALAE
                     RIAAQSVRLQMAADRTPVPRPGPR"
BASE COUNT          462 a          709 c          677 g          527 t
ORIGIN      
        1 tgttggctaa ggtggtcaag gtctgctgtc cgctttgctt gtgatagtgc gtggagcgga
       61 aagggcaaat cggcgagggg cgttcaggac aagccgtgcg gcaatgtcag ttccaattca
      121 ggaatcgccg gcggaaagca ctctcggcaa gtgaatcgct gcacatcagg ctaacggtat
      181 gacccgcaaa gagtcggaag acttttgatg gccgtgggcc tgggttgcca cggtcgatct
      241 cctggtgcag aaagcttatc gaaaggacat agtagtcgat gtgggaggaa ttgaatccct
      301 tttaaagaca agtacgtagc cccgcgctta tcacaccgtg ttccggtgga tcgccggcca
      361 gtcttcgata aggaccggga cgattgctgg cacgcctctc gattgcccgt tcgcggcgaa
      421 gaccacttac ggtgcggtcg cctggctggt ctttttcccc gcctcatttt gcgccgaaac
      481 ccattttgca tgttcctgcg agaccagccc ttcgatccgc tccgcgagca cggttatcgg
      541 cttatcagtg gattggtacg gcccggcttg cgcgaggtac aagtacttct cgtgtctgag
      601 ggattcgcaa gttgagcggt aggcaatcca atttgcctgg aattgattca gttgctgaat
      661 cccttctgcg agcacgatga ttatcccgag agccgcgacg taatgtcggc cccagttcgg
      721 caccgacaac actggcacga aaacggcgga gaccatggtg agcagtttca gccacttgta
      781 ccagcgttgg ttcactctgc tcttgttgtc gtaccagcta atctggtctt cgagacgatc
      841 caaggtcgga ttggactgcg agatagaggt cgacatcgtc cctgatttta cccggaattt
      901 ccgttttagt tcccaatggc cccacattct ctctttatct gggcccaggg cgcgggacag
      961 gagtccggtc tgcggccatt tgcaatctca cactttgagc agcgatgcgt tccgcgagtg
     1021 cggcggtctt cgctcgaacc cgttgatcca catcagcagc cgcatttact ccccacgcaa
     1081 ttggcattgc cgaggcgtct ccgtgtgtgt gcgccatctt gtcacgcatt ctcgtcgctt
     1141 cacgccgggc ccgttcggct tcccgcaggg ccgtggcgcg agcacattgg gcggcagcaa
     1201 ggttctgccg cgcgacctgc tcagcgacct tcgcttgcgc catcgcaatg ggcaggttca
     1261 tttcggccaa caactgcgct tcttccggat agcccgggcg caggtgcgtc cgtaccttgc
     1321 ccatgaagcg ggacgcggcg gaggcatcgg cattgcactt cgcctttgcg gccgctccga
     1381 cgtgttgacc gtacgacagc gaaacgtgtt cgagtgtccg aaatccgcga gagaacaccg
     1441 cgaaggtcgc tatgatcgcc agggccgcca ccagatatct gcgttccata cagcctcctc
     1501 gtacctctta agacgcgaga gccattcgat cgttaggatt tttttgtcca atgatcggcg
     1561 ttgctcaagt ccggtaaagt acacgtcgag tgccatcact ggctttccgc aagtgcttgc
     1621 tgccgctatg tttgctgttg ctggaagcgc tgcacgatgc tccggagcgt ccggaatcag
     1681 gatctcttgt ccaccagcgg acaagctggc gcagcaagag aatcactgct gctgaacagc
     1741 cccgcagaat tgtggacaag tatggatttg cggcttggag gttctctggg atttggcctg
     1801 cgttgctagc ggatcgtcac aaaaacttac ccctgcccgc cacggaccgg aaacttgcgg
     1861 gagagaaggg gaacccgaag gtctcacctt ctcccccgtg caacccaggt aggagaagcg
     1921 gcggttgcgc gctgccggga atgcgtcacc atccaatgca gaatctgacg tcggcggctg
     1981 atgacatgac agcaaccgcc atcccgatcg cagccgcaga cttaaccaag ctccagatcc
     2041 tgaaccagac aaacatctcg ggcctggggc aggcgaaccc gatgcagcag agcgtgctca
     2101 aactgctcca gcccgactcg agttcgctgc ccgccggcac cctgccgaag gtttcttatc
     2161 ggcggatcct acttcctgtt caccgaccac atcggcccgc tcgcactctg gctttaatcg
     2221 cgtctcgata gcctggcagt ccacagactg cgcctgcccg tacatgccgc attaagtgct
     2281 tctcatcgca tgcattgcaa tcgcccagcg gaaagaccgg ataggtgcgc agcctcgctg
     2341 actttcggag gtgtccgcag tgcttcctcc acgcc
//
LOCUS       DASRKK010000124         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_105509, whole genome shotgun sequence.
ACCESSION   DASRKK010000124 DASRKK010000000
VERSION     DASRKK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105509"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1030
                     /locus_tag="VFC15_02855"
     CDS             62..1030
                     /locus_tag="VFC15_02855"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZM09132.1"
                     /translation="MPDLYPFLLLPIFDERPWGVRDLRPIYTKIVKEPIGESWLTWGD
                     SCIANGPFAGRTLGEIAREYRRDLVGSAAVFEDRFPLLVKFLFPAEKLSVQVHPDDAG
                     AQRVGQPYGKTECWYVLQAENGAQVALGLRTGTTLEEFEKSIREYRAEELLNWINVYE
                     GDIIYVTAGTVHTIGGGMVLVETQQASDITYRLYDYGRPRELHIKEGIAAIKLDSKAG
                     KVVPDSQGDPNVLVRSPFFEVEKMKLHEPLQAFVSRKSPHIVVAVDGSGIIESAGMEP
                     ISFAKGEAVVVPANVPWYTVRPQWELEVMRMSLPTGDVAEPRTMLG"
     gene            1091..2200
                     /locus_tag="VFC15_02860"
     CDS             1091..2200
                     /locus_tag="VFC15_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HZM09133.1"
                     /translation="MGQSANNNFYPVILAGGRGTRFWPLSRKRRAKQLLPLNSARSMI
                     QETVDRLEPLVSSERFWIISNQDLRDPIVQQLSALDPNHVLAEPVGRNTAPAIGLAAF
                     LLERQDPDAVIGMFPSDHVISHEEAFRADVQRAVEIASSGANIVVMGIPPTRAETGYG
                     YIEVGQKSEQENFPVRGFIEKPEKEIAEKFVKAQNYFWNSGMFVWGARTLADALREHL
                     PNTAPVLEKIAASYGTAEFAATFVRLYPECENISIDYAVLEPRSKEGQGKSGIYCIPA
                     SFGWNDLGSWTALYEHRQANNPERDNVISSQRTFVLDASGNFVHSEKFVAVVGVNNLV
                     VVETEDALLITTRENAQDVGKVVKYLDSKDLTDLV"
     gene            2234..3664
                     /locus_tag="VFC15_02865"
     CDS             2234..3664
                     /locus_tag="VFC15_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZM09134.1"
                     /translation="MATHIKFGTDGWRGIIADDYTFENVRRVGSAIAHYVHENEDATK
                     GLVVGYDTRFGSRAFAEAISEVLAAAGINIHVSDDYTPTPALSFAVVNLGAAGGVMIT
                     SSHNPYNWNGVKYKAGYGGSATPAIIKSIEAALDAPVIQRPGGSVIEADFKTPYVAAV
                     KKFVDLDAIKKAGFKFAVDAMYGSGRGVLSQIFTEKGIDHIEIRGELNPLFPGINPEP
                     IIPHVTLLQETVVREHCQAGLVTDGDADRLGAVAEDGSFVDSHKIFSVILEWLLKRKQ
                     WPGEVVRAFNTTRMIDRISAKYGRKMHECGIGFKYICDLILERDILIGGEESGGIGIR
                     RHLPERDGILNSLLLANIMADEGKPLGQLVRDLQQEYGEHHYARTDLRLTQEVKEEAM
                     RRVEAKPAKLGSFNVLKVETLDGVKFFLDAPTNGNGAEAWVLVRSSGTEPLLRIYCEA
                     ASPELVSQILRETEAFVKSGDTTGAK"
     gene            3691..>4251
                     /locus_tag="VFC15_02870"
     CDS             3691..>4251
                     /locus_tag="VFC15_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09135.1"
                     /translation="MTTPANPQPGDSPETRRYNRIKRWLGMADAVIGFLLLVALLVTG
                     WTGKLRDWAYHDAGQHYFLAVFFYVLMFSMVMKVLSAPFDYLGFRLEHQYKLSNQKMR
                     SWLWDELKGWLVGLVLGTVFVEIIYAIIHIAPQRWWMIAWAVFVGLFLLMAQLAPVVL
                     FPIFYKIEPLNNDSLRERLTRLGERAG"
BASE COUNT          894 a         1119 c         1345 g          893 t
ORIGIN      
        1 cgtgcggacg tgagttcgcg actgaccacg cgtggtgtta gtcgccgaaa atcatccacc
       61 catgcccgat ctctatccat ttttgttgct gccgattttc gatgagcgcc cgtggggtgt
      121 tcgcgacctt cgtcccatct acacgaaaat cgttaaggag ccgatcggtg aaagctggtt
      181 gacatggggc gacagttgca tcgcgaatgg tccttttgcc ggccgcacac tgggtgaaat
      241 tgcgcgggag tatcgcaggg acttggtcgg atcggctgcg gtgttcgaag accggtttcc
      301 gctgctggtg aagtttctgt ttcccgccga gaagctgtca gtgcaggtgc atcctgacga
      361 cgcgggcgcg caacgggttg ggcagccgta cggcaagact gaatgttggt acgtgctgca
      421 ggcggaaaac ggcgcgcagg tcgcgctggg actcaggaca ggcacgacgc tagaggaatt
      481 cgagaagtcc atccgcgaat accgcgcgga agagttgttg aactggatca atgtctacga
      541 gggcgacatt atttacgtta cggccggcac ggtacacacc atcggcggcg gcatggtcct
      601 ggtggagacg cagcaggcct cggatatcac ttacagactt tacgactatg gccgcccgcg
      661 cgagctgcac atcaaggagg ggatcgctgc gatcaagttg gacagcaaag cgggcaaagt
      721 cgtgcccgat agccaaggcg atcccaatgt gctggtgcgt tcgccttttt tcgaagtcga
      781 gaagatgaag ttgcacgaac cgttgcaggc ttttgtttcg cggaagtcgc cgcacattgt
      841 tgttgctgtc gacgggtccg gaatcatcga gtcggcgggc atggagccca tcagtttcgc
      901 caagggtgag gcggtggtcg tgccagcgaa cgtgccttgg tacacggtga ggccgcagtg
      961 ggaattggaa gtcatgcgca tgtcactgcc aaccggcgac gttgcagaac cgcgcaccat
     1021 gcttggataa tcgattagca acctcatcgg agccagttga aagcctgcta ccgcgggtaa
     1081 ggaagtctcc ttgggccaat cagcgaataa caatttttac cctgtcatcc tcgcaggcgg
     1141 tcgtggcacg cgcttctggc cgctcagccg caagcgcagg gcgaagcagt tgctgcccct
     1201 caatagcgcg aggagcatga tccaggagac ggtcgaccgg cttgaaccgc tcgtgtctag
     1261 tgagcgcttc tggatcatca gcaaccagga cctgcgcgac ccgatcgtgc aacaactttc
     1321 cgcgctcgat ccgaatcatg tgctggctga acccgtcggg cgcaacaccg caccggcaat
     1381 cggtttggca gcgtttctgt tggagcggca ggacccagac gcagtcattg ggatgtttcc
     1441 gtccgaccac gtgatttccc acgaagaagc gtttcgcgcg gacgttcagc gtgcggttga
     1501 aatcgcaagc agcggagcca acattgtggt gatgggaatt ccgccgacgc gcgccgagac
     1561 ggggtacggc tacatcgagg tgggacagaa atccgagcag gaaaatttcc cggtgcgagg
     1621 attcatcgaa aagccagaaa aggaaattgc cgagaaattt gtgaaggccc aaaattattt
     1681 ttggaacagc ggcatgttcg tctggggtgc ccgcaccctg gctgacgcgt tgcgggagca
     1741 cctgccgaac accgctccgg tgctggagaa aatcgctgcg agctacggga ctgcggaatt
     1801 tgcggcgaca ttcgtcaggc tgtatcccga atgcgaaaac atcagcattg actatgcggt
     1861 gctggagccg cgctcgaagg agggccaagg gaagtcgggg atttactgca tcccggcgag
     1921 cttcgggtgg aatgaccttg gttcgtggac ggcgctgtat gaacatcgcc aggcgaacaa
     1981 tcctgagcgc gacaacgtaa tatcgtccca gcggaccttt gtattagatg cgtccggcaa
     2041 tttcgttcat tccgaaaagt tcgtcgcagt ggtgggagtg aataacctcg tcgtcgtgga
     2101 gaccgaggat gcattgctga tcactacccg cgagaacgcg caggacgtcg ggaaagtcgt
     2161 gaagtatctc gatagcaagg atcttacaga ccttgtctag ctgacgcagc ttgcgatagt
     2221 caggcaatca tttatggcga cccacatcaa atttggtacg gacggatggc gcggcatcat
     2281 tgccgacgat tacacctttg aaaatgtgcg gcgcgtgggc agtgccatcg cgcattacgt
     2341 gcacgagaac gaagatgcga ccaagggtct ggtggttggc tatgacacgc gcttcggttc
     2401 gcgagctttc gccgaggcta tctctgaagt gctggccgcg gccggcatca acattcatgt
     2461 ttccgacgat tacaccccca ctccggcact gtcgtttgcg gtggtgaatc ttggggccgc
     2521 gggcggggtg atgatcactt ccagccacaa tccgtacaac tggaacggcg tgaaatataa
     2581 ggcgggctat ggcggttccg ccacgccagc catcatcaag tcaatcgaag ctgcactcga
     2641 cgcgccagtg atccaacgtc cgggcggcag tgtaatcgag gctgacttca agacgcccta
     2701 cgtcgcagca gtaaagaagt ttgtcgatct tgacgcgatc aagaaggccg gattcaagtt
     2761 tgcggtcgac gcgatgtacg gctcgggtcg cggagtgttg agccagattt tcaccgagaa
     2821 gggcattgat cacatcgaga ttcgtggcga gctcaatccg ttgtttccgg gaatcaatcc
     2881 cgagccgatc atcccgcacg ttacgctctt gcaggagacg gtggtgcggg aacattgcca
     2941 ggcgggactc gtcaccgacg gcgatgctga caggttaggt gcggttgccg aagacggcag
     3001 cttcgtagat tcccacaaga ttttttcggt gatcctggag tggctgttga agcggaagca
     3061 atggcccgga gaagttgtgc gggcgttcaa taccacgcgt atgatcgatc gcatttccgc
     3121 caagtacggg cggaaaatgc acgagtgcgg catcggcttc aagtacatct gcgacttgat
     3181 tctggagcgc gacatcctga tcggcggtga ggagtcgggg ggcatcggta ttcgccggca
     3241 tctgccggag cgcgatggaa ttctcaactc gttgctgctg gccaacatca tggccgatga
     3301 aggcaaacca ctcgggcagt tggtacgcga tttgcagcag gaatacggcg agcatcacta
     3361 tgcgcgcacc gatcttcgtc tgacgcagga agtcaaagaa gaagccatgc gccgcgtcga
     3421 ggcgaagccc gcgaagctcg gcagcttcaa tgtgctgaag gttgagacgt tggatggcgt
     3481 caagtttttc cttgacgctc ccaccaatgg gaacggcgcc gaggcctggg tgctggtgcg
     3541 gtcctcgggc acggagccgc tgctgcgcat ttactgcgag gcagcgtcac ccgaactggt
     3601 ttcccagatc ttgcgcgaaa ccgaggcctt cgtgaaatcg ggcgacacca cgggagcaaa
     3661 gtagtgcagc tgtcagaatc agaatccgtt atgaccacgc cagcaaatcc gcagcccggc
     3721 gacagtccgg aaactcgtcg ctataaccgc atcaagcgat ggctcggcat ggccgatgcg
     3781 gtgatcggat ttctgctgtt ggtcgcgctg ctggtgacgg gctggaccgg gaaactacgc
     3841 gactgggcgt atcacgacgc gggccagcac tacttcctgg cggtgttctt ctacgtgctg
     3901 atgttttcga tggtcatgaa ggtgctgagt gcgccgttcg actacctggg atttcggctg
     3961 gaacatcagt acaaattgtc gaaccagaag atgcgttcgt ggttgtggga cgaactgaag
     4021 ggctggctgg ttgggctggt gctgggcacc gtgttcgtcg agattattta cgccataatc
     4081 cacatcgctc cgcagcgctg gtggatgatt gcgtgggcgg tgtttgtcgg attgttcctg
     4141 ctgatggcgc agttggcgcc cgtggtattg ttcccaatct tctacaagat cgaaccgctg
     4201 aacaacgatt cgctgcgcga acggctgacc aggctgggcg agcgcgctgg g
//
LOCUS       DASRKK010000125         5743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_790234, whole genome shotgun sequence.
ACCESSION   DASRKK010000125 DASRKK010000000
VERSION     DASRKK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790234"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VFC15_02875"
     CDS             complement(<1..1235)
                     /locus_tag="VFC15_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09136.1"
                     /translation="MPNATNCGVNPVLALELDRNGNVSKKTMKLDREAQLTNSLLQGL
                     YPGIHVATVDVPTEPLDSCEQAMVEQALTRIEIDGIRYGLVGASGSAKNGKFYAVESS
                     FEKKLAERFRFSPQAAITYFGILVSSCKVLVEEPDCRVMVVDDHEFGTNDCRGWISQS
                     LFRKLQQKHQCELLARETERLHAQRIAERSEEQQDLPLTEEEEAALVEQAKRRIHGRQ
                     IGAHRFYQFRLAFDRTQAKGAFKVMADEVGRHLDADIILPRSCVKPKYQGKVLRTIRS
                     LMGDRQAHTFRGPVIVGFRDVSRNLEFNSSYTLLEHAPEDSIELEIKPYALSQIEKVR
                     QAFEANNFTELFELLGTKESQRVLEPGEETDPDYTSNEYTVAQAALIADATGYMLKHP
                     FIQHHLQRVLMKWAYRLCT"
     gene            complement(1374..1685)
                     /locus_tag="VFC15_02880"
     CDS             complement(1374..1685)
                     /locus_tag="VFC15_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09137.1"
                     /translation="MVKQRFRVLFRRWFFTVAKFCGPILLFELFVLVRQHFQYVPPSI
                     SDLLMGALLAFLLTALLCWLWALGEWLWFRLRRLRGFLGELRQCSRSYWNPEAVRTNK
                     A"
     gene            complement(1897..2223)
                     /locus_tag="VFC15_02885"
     CDS             complement(1897..2223)
                     /locus_tag="VFC15_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09138.1"
                     /translation="MTGTAATDTVPAAKANRTDSGTEKTVTRTRRSRTNSDEEANGNA
                     RYFLAKTNSASGTPALDREVTSEGEALVEALRLGVTYYEVQEFRVVPDFAGRKPQLNK
                     EAVNGK"
     gene            complement(2308..2730)
                     /locus_tag="VFC15_02890"
     CDS             complement(2308..2730)
                     /locus_tag="VFC15_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09139.1"
                     /translation="MRRFLEHYLSYTPDSAPRNLAESLNMMTNNLRTFTLNKLRDDDT
                     VGKIQQDHIISDLRIRSIERSKNAPWDYVVFGVKEVHKLKSGAEVTDRIVGQYNIRLV
                     EERRSEFNPSGLLVAEYSEQQMVGERNNGLEQKSELDK"
     gene            3002..3577
                     /locus_tag="VFC15_02895"
     CDS             3002..3577
                     /locus_tag="VFC15_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09140.1"
                     /translation="MFTWQGSEGIHASVLPQLISGFLGALIGAAVTVGGIVYNQRRGT
                     EALRRWLTEAHFDLLQDVADCAGEYERMGNFGIFRKRLSPQVRQLRDGLYRDSTGGLE
                     KEEYRRVYRSLSDVDYMIFVHDRNFAPNLMVSYINECINQMREAQELFGSFPKARPNA
                     ETDIMAGKQITYELPERGAERSFPPEPLDRC"
     gene            complement(3709..5028)
                     /locus_tag="VFC15_02900"
     CDS             complement(3709..5028)
                     /locus_tag="VFC15_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09141.1"
                     /translation="MIREWPIDEIKQAHSKLYERAIRDGRVSKPRQAVLDEVDELLSQ
                     IRLCSQSVRTIDDYRWLSQAALQWQVVYSSTWNIAKTVEIPGPPKALLQPLPSDKALS
                     ETELESWRNRHADYIGLLRFVEQRRESSPEEMEWDWYQAQVILVSDILDGFPNFAGRI
                     AAESYWRLDQVWLKEVKRIRAYFIWEGEGRKVFDPEEKNYDRACEHIRDMLVNKEIKA
                     QAIEFGVAKAYLEGRYLTGGRVVHEGTSEKCASAKVLIATKAERIRERRKWTESGYIE
                     SSQNDLTNWLRAETYVKLFYENIIPAVVEKDRMSVLRVLKAFQYSKAPENCWWVINGF
                     ETALAIYFLDCEIIESFWADSENADPLPASYINSSMQVSWGLREFHVPAICKDSFTFD
                     GKQIAFRGVMTDEQKEALLEQLQEEDYREDIELLCRRSRLIPRRTTL"
     gene            complement(5034..>5743)
                     /locus_tag="VFC15_02905"
     CDS             complement(5034..>5743)
                     /locus_tag="VFC15_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09142.1"
                     /translation="ATVRMSLPSDLAGVTCLNYTPFTSSVDLTPALGPACFRIKQEIR
                     QQGPRTVAGPIPRYLWRDYLDILAFAKNMHTSFQALDTSHLDSLSQTVWDLVAGNVSA
                     KSRLSKKMESIQAWNQYITQFTAHRLPKDADWDSLDRSFQKLHDNLSLDVRPEIVKCR
                     DFYGSIKTHQADFLSAHDAAILILGTGTLIELLDKPDQGTTFWVSLDRLSKTGKGLIT
                     QADAMIVNLVNLIADSR"
BASE COUNT         1234 a         1647 c         1467 g         1395 t
ORIGIN      
        1 ctggtgcaga gccgatacgc ccatttcatc aacacccgct ggaggtggtg ctgaatgaac
       61 ggatgcttca gcatgtaccc ggtagcgtcg gcgatcagag ctgcctgcgc caccgtgtat
      121 tcgtttgagg tgtagtcagg atcggtttct tcgccgggct ccaggactcg ctggctttcc
      181 tttgtgccca acagttcaaa tagctcggtg aagttgttgg cctcgaacgc ctgccgtact
      241 ttctcgattt ggctaagagc gtacggtttg atttccagct caatcgagtc ttccggggcg
      301 tgttccagca gggtgtaact cgaattgaat tcgaggttgc gggaaacgtc acgaaagccg
      361 acgatcactg ggccacggaa ggtgtgtgcc tgacgatctc ccatgagcga tcgaatcgtc
      421 cgcaacactt ttccctggta cttgggcttc acgcacgatc tgggcaggat gatgtccgca
      481 tcgagatgcc gcccgacttc atccgccatg accttgaatg cgcctttcgc ctgggtcctg
      541 tcgaacgcca gacgaaattg ataaaagcga tgcgccccga tttgcctgcc atgaatcctg
      601 cgcttcgcct gttcaaccag cgccgcctcc tcttcctcag tcaacggaag gtcctgctgc
      661 tcttcagatc gttctgcgat ccgttgcgca tgcaatctct ccgtctccct ggctagaagt
      721 tcgcactggt gcttctgctg gagtttccgg aacaacgact gcgagatcca tccccggcaa
      781 tcgttggtgc cgaattcatg gtcatcgaca accatgacgc ggcaatctgg ctcctcgacg
      841 agaaccttgc acgaggacac caggattccg aagtaggtga tggcggcctg cggtgagaaa
      901 cgaaacctct ccgccagctt cttttcgaag gaactctcga ccgcgtagaa cttgccgttc
      961 tttgcgctgc cgctggcccc aaccaagccg taccgaatgc catcaatctc gattcgggta
     1021 agtgcctgct cgaccatggc ctgctcgcag gaatccagcg gttccgtcgg cacgtcaacc
     1081 gtggcgacat gaatccccgg atagagccct tgcaagaggc tgttggtgag ttgcgcttcc
     1141 cgatccagtt tcatggtctt cttcgatacg tttccgttac ggtcaagttc gagcgccaac
     1201 accgggttga cgccgcagtt cgtagcattt ggcattgcga atctccttta agctgtcgag
     1261 caaggacagc cggaggagac ggctcgtgcc agtctcttcg ctgcccttgc cgaaaaccga
     1321 accaaaagat ttcggccagg cagctaagcg ccggccaaca cgagttgtcg ttgctacgcc
     1381 ttgttggtcc tcactgcctc aggattccaa tagcttctgg agcattgccg tagctcgccc
     1441 aagaaaccgc gtaacctgcg cagcctgaac cacaaccact ctcctaacgc ccacagccaa
     1501 cacaacagag ccgtgagcag gaaagccaaa agtgctccca tcagcaaatc cgaaatgctg
     1561 gggggcacat actgaaagtg ctgccggacc aacacaaaca actcgaacag caggattggt
     1621 ccgcagaact tcgctaccgt gaagaaccaa cgtcggaaca gcacccgaaa ccgttgcttc
     1681 accatctttt cctccttttc tttctgcgta ttgccagaac agaaaggagg gacgctggcg
     1741 cggctctctc actgtcctgg tcgaaaacgt ttcaggttcc cggccaggct cgcgaaacga
     1801 accagaccag cactcaaccg caatcagctt gcggatcgct caccacagat caacatcatc
     1861 tgtaaatgaa gccagtgcta taaaaactcc tggactctac ttgccgttga cggcttcttt
     1921 gttcagttgc ggcttccttc ccgcaaaatc cggcaccaca cgaaactcct gaacttcgta
     1981 gtaggtgacc cccaagcgga gcgcctcgac cagcgcttcg ccctcgctcg tcacctctcg
     2041 atccaacgcc ggggtgccac tggcgctgtt tgtcttagca aggaaataac gcgcgtttcc
     2101 attggcttcc tcgtccgagt tggtacgaga acgtcttgtt cgggttaccg ttttttcggt
     2161 gccgctgtca gttctgttcg cttttgcggc cggaaccgtg tctgttgccg ccgtgcctgt
     2221 catgcttgac tcctattgcc tgaaacctgc atcaagttgc atgtcgccac ttctggattc
     2281 accagattgc ctctggttgg gcgccttcta cttgtcgagc tcgctctttt gttcaagccc
     2341 attgttccgc tcaccgacca tttgctgctc ggagtactca gccaccagca gtccgctggg
     2401 attgaattca cttcgccgct cctcgaccag ccgtatgttg tactgaccaa caatgcggtc
     2461 cgtcacctcc gcaccgctct tcagcttgtg tacttccttg acaccaaaca cgacgtaatc
     2521 ccaaggcgcg ttcttggagc gctcgatcga gcgaattcgg agatccgaaa tgatgtgatc
     2581 ttgctggatt ttgcccacgg tatcgtcgtc gcgcagcttg ttcagtgtga aggtccgcag
     2641 gttgttggtc atcatgttca gcgactcggc aaggttgcgc ggcgccgaat ccggcgtgta
     2701 ggagaggtag tgttcgagga accgccgcac gacggctttg ccttccagat ccgtcgtcgc
     2761 gatgccttcg gcgacaggcg cgtttcagcg cggcgagttg taacgccgtc gtctggtcgt
     2821 cggtgctggt ttgggcgtag ccgtatctca tcccgcccca tcgtgactcg ggcctttgac
     2881 acacgacaac cccgcgactt aaggtcattc cgcgccacgg ggattacgga ttacctgacg
     2941 aacgggaggc gcatcgacgt tgcgcaaaag acggtccgat gcagtagagg agctggtacc
     3001 aatgtttacg tggcaaggta gtgagggcat ccatgcttcg gtgctccctc aattgatctc
     3061 agggtttctt ggtgccctga taggcgccgc agtgaccgta ggcgggatcg tttacaacca
     3121 gcgccgtggg acggaggctc tccgccgatg gttaactgaa gcgcatttcg atctactcca
     3181 agatgtggcc gactgcgcgg gtgaatacga aaggatgggt aactttggaa tattccgtaa
     3241 acgactgagc ccccaagtgc gacaattgag ggacggtctt taccgagact caactggcgg
     3301 cctagagaag gaggaatacc gccgggtata tcgctcgttg agtgacgtcg attacatgat
     3361 tttcgttcac gaccgtaact ttgcgccgaa cctgatggtg tcatacatca atgagtgcat
     3421 aaatcagatg cgagaagcac aggagctttt tggaagcttc ccaaaggcta ggccaaatgc
     3481 cgaaaccgac ataatggcag gcaagcaaat tacttatgag ttgcccgaac gtggggctga
     3541 gcgatcattt ccacccgaac ctctcgatcg atgctgatgt gacacgacgt gtagtcgcca
     3601 gctctttcat aatcccgtct tcgcccccgg caatcgcgct taacgggctt cggcggcgtc
     3661 gcaccaagca ccgtctttgc gattgctaaa atcctgtcct gactggttct acaaagtggt
     3721 tcttcggggg ataaggcgac ttcgacgaca gagcaattca atgtcttcgc gatagtcctc
     3781 ttcttgcaac tgctcaagga gcgcctcttt ttgctcatca gtcatcacgc ccctgaaagc
     3841 aatctgtttc ccgtcgaacg taaaactatc cttgcatatc gccgggacat ggaactcccg
     3901 taagccccag gaaacctgca tactgctgtt gatataggag gcaggcaagg gatctgcatt
     3961 ttcggaatcc gcccaaaaac tctctattat ctcgcaatcc aggaaataaa ttgccaaagc
     4021 tgtctcgaaa ccatttatga cccaccagca attctcgggt gctttgctgt attggaacgc
     4081 cttgagcacc ctcaaaacac tcattcgatc tttctcgacc acagcaggaa taatattctc
     4141 ataaaatagt ttcacatacg tttccgcacg gagccaattc gtgagatcgt tttgagaact
     4201 ctctatgtag cccgactcgg tccacttcct cctctccctg attcgctccg ccttagtggc
     4261 aatcaatacc ttcgcactag cacacttttc gcttgtgccc tcgtgcacaa cccttccgcc
     4321 tgtcaagtat cgcccttcca ggtaagcctt cgctaccccg aactcaatag cttgagcctt
     4381 gatctccttg tttaccagca tgtcccgaat gtgttcgcac gcgcggtcgt agttcttctc
     4441 ttctggatca aagacctttc tcccctcgcc ttcccaaatg aagtaagccc taattcgctt
     4501 gacctccttt aaccatacct gatccaatcg ccaatacgat tcagcagcga tgcgaccggc
     4561 aaagttgggg aacccgtcaa gtatgtccga gacaagaatg acttgggcct ggtaccagtc
     4621 ccactccatt tcctccggac tgctctcgcg tctttgctcg acaaatcgca gcaggccaat
     4681 gtagtccgcg tgcctgtttc gccaactctc aagctccgtc tcagagagag ccttgtcgga
     4741 tggaagtggc tggagaaggg ccttcggagg tccaggtatc tcgacagttt tcgcgatgtt
     4801 ccaggtggat gaatagacta cctgccactg cagagcggcc tggctgagcc agcgatagtc
     4861 gtcgattgtt ctcacactct gggaacagag tctgatctga ctcaacaatt cgtctacttc
     4921 atcgagaaca gcttggcgcg gctttgaaac tcggccatcc cgaatggcgc gttcatagag
     4981 tttgctgtgt gcttgcttta tctcgtcaat tggccactct cttatcatgt gggttacctg
     5041 ctgtcagcga tcagatttac aagattgacg atcatggcat ctgcttgcgt aattaggccc
     5101 ttgccagtct ttgatagacg gtcgaggctg acccagaacg tcgtaccttg gtccggctta
     5161 tccaagagtt caatcagggt tcccgttccg aggattaaaa tagcggcatc atgcgccgag
     5221 agaaaatcag cctgatgagt cttaatcgaa ccgtaaaaat ccctgcactt cacaatctcc
     5281 ggccttacgt ccaaactcaa gttgtcatgg agcttctgaa acgatctatc gaggctatcc
     5341 cagtcggcgt ctttgggaag tcgatgagcg gtgaattggg taatatattg gttccaggct
     5401 tggatggatt ccatcttctt gctcaggcgt gatttcgcag agacattgcc ggcgactaaa
     5461 tcccagaccg tctgactgag agaatcgagg tggctagtat ccagggcctg gaagctggta
     5521 tgcatgttct ttgcaaaggc gaggatgtct agataatccc tccataaata gcgaggaata
     5581 ggacctgcaa cggtccgagg cccttgctgt cttatctcct gtttaatcct gaagcatgcg
     5641 gggcccaagg ccggtgtaag gtcaactgaa ctggtgaacg gtgtgtaatt gaggcaggtg
     5701 acgccagcca agtcggaagg aaggctcatg cgtaccgtgg cat
//
LOCUS       DASRKK010000126         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_887821, whole genome shotgun sequence.
ACCESSION   DASRKK010000126 DASRKK010000000
VERSION     DASRKK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887821"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1805
                     /locus_tag="VFC15_02910"
     CDS             324..1805
                     /locus_tag="VFC15_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HZM09143.1"
                     /translation="MRSPFDPQLRLGSTAISDIPLNTRCRDEIIPILTALKHIYGQPA
                     LRDEILVAVAHDVNACSSPDRGRPGMDYWPILVLAAVRLGCNLNYDKLQNLAEEHRTL
                     RAIMGIDGWDRDHTFDWRLLHDNISLVSPDTIEQINQRIVAEGHRLVPTAVETVRGDS
                     FVLETNIHYPTDSSLIGDGLRIIVRTAVRLANLLGVSGWRQHHHLMGSVKTHLRLINK
                     IAKSKGRNSETRLEEAYRTLLDEADRILARALNLLEPERISIVPSGFEKRVARLRGKL
                     LDALDRTIHVCDVARRRVLKGKTIENREKLFSLFEPETQLINRGKVPHPIEFGHRVLV
                     VEDGAGFVCYYAILPHGVEDRAVAVETMKQVQERFHGQVRSASFDRGFHTPENQIALS
                     KLVLHPCLPKPGHRQSEQQEREATVEFRAARRRHPGVESAIGALQSGNGLDRCRDHSF
                     GGYSRYVGLGILGRNLHVLGKILIAREDKTRVAGQTKRGRVAA"
     gene            2064..2612
                     /locus_tag="VFC15_02915"
     CDS             2064..2612
                     /locus_tag="VFC15_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09144.1"
                     /translation="MPRGRPGSKAARRAARRKKRIEQKVRDLFEHRYLFVRHHLTDSE
                     RKTLARITRGQPQLRNLRGLMEEVYRLFDRRCRTATALAKLLRLRARLRRFVRLRVVL
                     KKLFLPGLEKALVFLDEKLLGATSNAVERGNRRYRKMQKSVYRVRTQHSIEARLALDL
                     LRERQARSRAATTKTLHRARAA"
BASE COUNT          547 a          829 c          835 g          541 t
ORIGIN      
        1 tggccactct ccgcgcctta ctgaaccatc ccccgtccga gaggggctga tcgagcccgg
       61 tccctctcat caggacgtct cggcccacgt ccccccgagc ctccttacaa cgcactgctc
      121 gcgcaagctt gccaggttct catcgacatt catttgagtg taactagtgt ccgtcggata
      181 actggcgaaa agctatgttt ttcggggttg gcggctgatt ttctcctcga agatcgctgg
      241 gatttcgcgt atcaatcctg aagaaatgcc gatttccgtg gaattccgcg ttcccagcgg
      301 tccattcttc gagagacttc accatgcgca gccccttcga tccccagctt cgactcggtt
      361 ccaccgccat ctccgacatt cctctcaata cgcgctgtcg cgacgagatc attcccatcc
      421 tcacggcctt gaagcacatc tacggtcaac ccgcactccg cgacgagatc ctcgttgccg
      481 tcgcacacga cgtcaatgcc tgctcaagtc cggatcgtgg ccgacccggc atggactatt
      541 ggcccatcct cgttctcgct gccgtccgac tcggttgcaa tctcaactac gacaaactcc
      601 agaacctggc cgaggagcac cgcacactcc gcgcgatcat ggggatcgac ggctgggatc
      661 gtgaccacac cttcgactgg cgcctccttc acgacaacat ctctctggtc tctcccgaca
      721 ccatcgagca gatcaaccag cggatcgtgg ccgagggaca tcgactcgtc cccacagccg
      781 tggaaacggt ccgcggcgac tcgttcgtcc tcgaaaccaa catccactat cccaccgatt
      841 ccagcctgat cggcgacggt ctgcgaatca ttgtacggac cgccgtgcgg ctcgcgaacc
      901 tcctcggagt gagcggctgg cggcaacatc atcatctgat gggcagtgtt aaaacacatc
      961 ttcgattgat caataagatc gcgaaatcca aagggcgcaa ctccgagacg cggcttgaag
     1021 aggcctaccg gacactcctt gatgaggccg accggatttt ggcccgcgcc ctgaacttgc
     1081 tcgaacccga gcggatctcg atcgttccca gcggtttcga gaaacgggtg gcccgactcc
     1141 gaggcaaact gctcgatgca ctggatcgga cgatccatgt ctgtgacgtg gctcgacgcc
     1201 gggtcctcaa gggcaagacg atcgagaatc gcgagaagct gttcagtctg ttcgagcccg
     1261 agacgcaact gatcaatcgc ggcaaggtcc cccatccgat cgagttcggt caccgcgttc
     1321 tggtggtgga agatggtgcg ggcttcgtgt gctactacgc gattctgccg cacggggtcg
     1381 aagatcgtgc ggttgcagtg gagacaatga agcaagtgca ggagcggttc cacggtcagg
     1441 tccgtagtgc ctcattcgac cgggggttcc atactccaga gaatcagatc gcactgagca
     1501 aattggtgtt gcatccatgc ctgccgaaac caggacaccg tcaatccgag cagcaagagc
     1561 gagaagcgac cgtggagttt cgcgcggcgc ggcgtcgcca tccgggggtg gaatcggcga
     1621 tcggagcgtt acagtcaggg aacggactgg accggtgccg cgaccacagc tttggtgggt
     1681 acagccgcta cgtgggtctg gggatcctgg ggcgcaatct tcatgtgttg ggcaagatcc
     1741 tgatcgcccg cgaggacaag acaagagtgg cgggtcagac caagcgcggc cgagtcgccg
     1801 cctgaaacgg tgattgaggc gaaaaccgcc gctggaacca ccgggttagc cgagtggagt
     1861 ggagaaggtg cgcttcaagg ttggccaata gtagtaaacc cgagtcgatt tttggataat
     1921 tttgttgtca cgttaaagat cattggagcg aacggtgtca ttaccctgcg attccaccag
     1981 ttcttgactg gcatcaaggg cggaatgcgt gtttttcgga cggacacaag ctacgcaagg
     2041 acctggcggc tggtgccccc aagttgccgc ggggccgtcc cggctcgaag gcggcgcgtc
     2101 gagccgcccg ccgcaagaag cggatcgagc agaaggtccg cgatctgttc gagcaccgct
     2161 acctgttcgt ccggcaccat ctgacggatt ccgaacgcaa gaccttggcg cggatcaccc
     2221 gtggccaacc acaactccgc aacctgcgcg gtctgatgga ggaggtctat cgcctattcg
     2281 accggcggtg ccggacggcg acagcattgg ccaagctttt acgactgcga gcgcggttgc
     2341 ggcgttttgt ccggctgcgg gtggtgctga agaagttgtt cctgccgggc ctggagaagg
     2401 cgttggtatt ccttgacgaa aagctcttgg gagcaacgtc aaacgcggtg gagcgtggga
     2461 accgtcggta ccggaagatg cagaagtccg tgtaccgggt gcggacgcag cattcgattg
     2521 aggctcgtct ggcgctggat ctgctcaggg agcgacaagc tcgaagccga gccgcgacca
     2581 ccaagaccct ccacagggcg agagcagcgt gatcaaatga cccggtatcg ttgctacagt
     2641 gtcggtcagg ccgggggttg ggtcacgttg cgaggcgttc cgggttcctc gatcagctcc
     2701 cggtgccggg gcgcttcggg cccgactggc cgggggcgag gcggcggtac tt
//
LOCUS       DASRKK010000127         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_985527, whole genome shotgun sequence.
ACCESSION   DASRKK010000127 DASRKK010000000
VERSION     DASRKK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985527"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFC15_02920"
     CDS             <1..202
                     /locus_tag="VFC15_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09145.1"
                     /translation="QSQIETDVDAIEALGVKPILGEYLLEAMDSREGLVARHDTHRVA
                     HDLLQLMMELRESPVAALHHEP"
     gene            323..748
                     /locus_tag="VFC15_02925"
     CDS             323..748
                     /locus_tag="VFC15_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09146.1"
                     /translation="MIVKAKCIAEAFDSKNAWLYHAGDVVDLDLSDPNKRKLADLTTP
                     TGKWIFEFDRAGSSDPKINLFFCEKCGQRFGALNELRTHSRSVHKKPARLSPVVVLEK
                     TVKPWWERWKWIAALVSLVLTIIGWIISHVGWIISRFRH"
     gene            915..2348
                     /gene="glmU"
                     /locus_tag="VFC15_02930"
     CDS             915..2348
                     /gene="glmU"
                     /locus_tag="VFC15_02930"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZM09147.1"
                     /translation="MPKKQSQQQQGFAIAILAAGKGTRLKSRRPKVLHEIAGKPLLAH
                     VVAAASEVVPPQNIFAIIGYEAERVREALAPTGINFVLQREQRGTGHAMMEARNTLKN
                     FETVLVLSGDVPLIKAETIKRVCDFHNAHHAAMTILTAEPPDPTGYGRVFRKQGKGNA
                     SGEVERVVEQKSLRGTEAEQREINSGIYAFATKPLYTHIGKLKTDNAHHEYYLTDMVA
                     LLSKAGEKVLALHADDSSEVLGVNTRIELAELDAKLRDQKARELMLAGTTIFRPQTCD
                     IDADVEVGPDTVIEPFVQLIGKTKIGADCRIRSYSVITNCEIGDGVLIRPGCILEDSV
                     VRNGAQLGPYSHLRQGSDIGEGAHVGNFVETKKTRLGKGSKANHLTYLGDAEIGEGVN
                     VGAGTITCNYDGVNKHGTAIEDGAFIGSDATLVAPIRIGKGAYVGAGSCITQDVPADA
                     LALGRGRQVTKEGWAAERRAKLKNTSK"
BASE COUNT          588 a          701 c          773 g          460 t
ORIGIN      
        1 gcagtcgcag atcgagaccg atgttgacgc catcgaggcg ctcggggtga agccgatcct
       61 gggcgagtat ctgctggaag cgatggattc gagagaagga ctggtagccc gccacgatac
      121 gcaccgtgtc gcacacgacc tgctgcagct catgatggaa ctaagggaat cgccggtagc
      181 ggcactgcat cacgagccgt agaagaggat tcaccacaga gacacagaga gcacggagga
      241 ttgctagtat cgagttccga cctttgggag agggcccttt aattcgcaca taggagtggc
      301 gcggtggcga gtcggagatt ccatgatcgt caaagcgaaa tgcatcgctg aagcctttga
      361 tagcaagaat gcgtggctat atcacgccgg tgacgtggtc gacctcgatc tgagcgaccc
      421 gaacaagcgc aagctagcag atttgacaac ccccacggga aagtggattt tcgagttcga
      481 ccgggcagga tcatccgatc ccaagatcaa cctatttttc tgcgaaaagt gcggccagcg
      541 tttcggggcc ctcaacgagc tgagaacgca cagcagaagc gttcacaaga agcctgccag
      601 actttcgcct gttgtagtgc tggaaaagac cgtgaaaccg tggtgggagc gttggaaatg
      661 gatcgccgcg ctggtaagcc ttgtgctgac catcattggt tggatcatta gccacgtcgg
      721 ttggatcatt agccgcttcc gtcactgaag actcagattt atgcgcaagg ttcaattgca
      781 tcttcgacgg cttcgactgc agcatcacag ctttgcttat agcggaggaa ctcccaccgg
      841 cctgcgatgc cgccttacag atgggaattt cctctgtgtc tccgtgcctc tgtggttaaa
      901 tgaaatgctt ggacatgcca aagaaacagt ctcaacaaca gcagggcttc gctattgcca
      961 ttctcgccgc gggcaaaggc acgcggctga agtcgcggcg cccaaaggtc ctgcacgaga
     1021 tcgcgggaaa gcccctgttg gcgcacgtgg tggcggccgc cagcgaagta gttcctccac
     1081 agaatatctt cgcgattatc ggatacgaag ccgagcgggt tcgcgaggca ttggccccaa
     1141 ccggcatcaa cttcgtcctg caacgcgagc agcgcggcac cgggcacgcc atgatggaag
     1201 cgcgcaatac gctaaagaat ttcgaaacgg tgttagtgct ctcgggcgat gtgccgctga
     1261 tcaaggcgga gacgatcaag cgggtctgcg attttcacaa tgcgcaccat gccgccatga
     1321 ccatactcac cgccgagcca cctgatccca cgggatacgg ccgggttttc cgcaagcagg
     1381 gcaaaggcaa cgcgagcggc gaagtcgagc gcgtcgtcga acagaaatcg ctgcgtggta
     1441 cggaagcgga gcaacgcgag atcaactccg gcatatacgc ttttgcgacc aagccgctct
     1501 acactcatat cggcaagctc aagaccgaca atgcccatca cgagtactac ctgacagaca
     1561 tggtagcgct gctgagtaaa gccggcgaga aggtgctggc gctgcatgcc gacgactcca
     1621 gtgaagtact cggcgtcaac acgcggatcg aattggcaga gctggacgcc aaacttcgcg
     1681 atcagaaggc ccgcgagctg atgctggccg gcacgaccat cttccgtccc cagacgtgcg
     1741 acatcgatgc cgacgttgag gttggcccgg acactgtgat cgagccgttc gtgcaactca
     1801 ttggcaagac gaagatcggc gctgactgcc gcattcggtc gtactccgtc atcaccaact
     1861 gcgaaatcgg cgatggagtc ctgattcggc cgggatgcat tctggaagat tccgtggtac
     1921 gcaacggcgc gcagctcgga ccctattcgc atctgcgtca gggcagcgat atcggcgaag
     1981 gcgcacacgt cggcaacttc gtggagacca agaaaacgcg actgggcaaa ggttcgaagg
     2041 ccaaccacct cacctatctt ggcgacgccg aaattgggga aggcgtgaat gtcggggctg
     2101 gcaccatcac ttgcaattac gatggagtga ataaacacgg cacggcgatt gaagacggag
     2161 cgtttatcgg aagcgatgcg acgctggtgg cgccgattcg gatcggaaag ggcgcctacg
     2221 tgggagcggg atcgtgcatc acgcaagatg ttcccgccga tgcactggct ctcgggcgcg
     2281 gccgccaggt caccaaagaa ggctgggcgg cggagcggag ggccaagcta aagaacacga
     2341 gcaaatagtt gtgcgcgaag ctgaattttt cacgaggaac aaaagcttat gttgtcatcc
     2401 cgagggaggg cgcgcttcgc ggcccgagtc gagggatctg tggttcgccg gtgccgtcgc
     2461 gccgccggca acaaacgcag atccttcgac tgcgcgcccg cccgacccgc aaaggaagcg
     2521 gg
//
LOCUS       DASRKK010000128         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_985591, whole genome shotgun sequence.
ACCESSION   DASRKK010000128 DASRKK010000000
VERSION     DASRKK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985591"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFC15_02935"
     CDS             complement(<1..302)
                     /locus_tag="VFC15_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09148.1"
                     /translation="MIFFRSFRAAVVCALAALLFNCQVSASTGRSHEITNIHFENVTQ
                     TTVDVVWNTVHPSTSQVLIARDTNYEPERWAPATADAALATTHRVTVDHLLPYNQPA"
     gene            2011..3099
                     /locus_tag="VFC15_02940"
     CDS             2011..3099
                     /locus_tag="VFC15_02940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM09149.1"
                     /translation="MERCAIQLATSAIGAGYDARLILYDVPPRRSGAEYDPGNVPVAF
                     VSRRHGIDITLPVRLARIFQSWNVDIVHARNNVAGFYSAAALGLIQDPPPLILTFDTF
                     PGHGTAKARLASRWASQCAAIVSSVSSDLSDRLVRSGWVNRCETVWNGVDTGAFNPDG
                     PLHGFKKRFGFPQHTLVIGNVARLDENKRQLDLIEAFSRLTRENSGIALAIAGDGPNR
                     ERVVKAASSVRNVLMLGRIYDVAAFLREIDTFVLCSEDEGCPRALLEAMACRKPIIAT
                     AVGGIPHILAGCGLLVPPRRPDMLADAINVMCSSREVREQFGQLARARILDSFTLEQE
                     WCHYERMYESVIQLPTNSLAVGRLARPD"
BASE COUNT          869 a          959 c         1051 g          819 t
ORIGIN      
        1 gccggctgat tgtacggaag cagatggtcc acagtgacgc gatgagtggt ggccaacgcc
       61 gcatcggcgg ttgctggggc ccaccgttcc ggctcgtagt tggtatcacg cgcaatcagc
      121 acctgagatg tgctggggtg caccgtgttc cacacaacat cgacggtcgt ctgggtgacg
      181 ttctcgaaat gaatgttggt gatctcgtgg gaacgtccgg tgctcgccga gacctgacag
      241 ttgaacagca aggccgccaa tgcgcacact acggcagcac gaaaggatcg aaagaaaatc
      301 atgatgcaag aatctcccgg gcgctctgat aacacatgag gatcgattgc cggcgcgatg
      361 cgcttgcgaa ctgcgtcggc gagagcatcc cctagttaat tgacgtgccg gaaacctaaa
      421 tgtggactta tcgacgtcta ttgtcaagag cgagtaagag cagactacag gcggagtaaa
      481 agtgaaccgg aggagttggc aagagatcgg gaatggtcag ccgttccgat ccgagcttca
      541 gtccactcct agaacgggag attgctggtc accagggtcg tgccgcgttc gtaggcgggc
      601 tgaagacctc gaatagcagt tcggcgccgg acttcgatag cggtacgaat cccaggtcat
      661 cgaccatcag cgacgtagca gtcccaaagt tttggttcgt tgcggttctg tgactagcat
      721 cacagcaatc cttactgtca tagacgagaa ttgtttcctc cctcgacgtt cggagcagct
      781 cagagttgac aggggtaggc ccgtgaacac ttactcgaac cagacccatc cggaccgctc
      841 tatccgtgga cgaaacggtt ttcgctctta tgtcagccaa aggcccacgc tgaagcgaat
      901 acttctgcca ttggtggaat gctcggagat ggccctcaat atcgtcggca gacgcttccc
      961 gtcgctgata aaaccaagaa ccggcaacat ttcactggcg attacttcgc agtgcaacct
     1021 gagatgcctt ggatgccgtt acggccgaga tttcatgctt ggggctcggc tggatctcac
     1081 ctcagtgaaa aacctgatcg atgatgccgc ggctttgggc atccgctgga ttcgattcta
     1141 cggcggggaa cccctagtgc acaccgactt gggctctatg atctcatatg caacgaatcg
     1201 aggtgttcac tcctggatca gcacgaatgc agctctattg aatggcccta aattccacga
     1261 gcttcgtaga gcgggattgc atggagtggc tatcggactc tacggagtga acgaagagta
     1321 tgactggtat gtgcaaaaga aaggcgcatt cgagactctt gagcacaact taaaagagct
     1381 acgcaagggt cccggccagg atttcgacct tcacttctct tggttattag ctgctcatac
     1441 ttgcaaagtt gacgctctcc gtgaggcctg gtcgctcgcc gaacgattca acgcgcattt
     1501 tcacgttgat atcgtgcacg aaggtggctc gttgccatac tttactgacg ggccccatgg
     1561 ctgcttaaga cttgatgctt cgtgcagaca actcgaagaa gtagtaaccg aattactgcg
     1621 attgcgacgg agtagtccga ttcgatatcg cgaacccata gctagcattc gggcgatacc
     1681 agattgggtc ggcactggaa agcagatgga tgtgccttgt gatatgtaca agcacatctg
     1741 gatcggccca gacggatctg taaagctttg ctactcgggt ttcgcactcg gaaatataaa
     1801 tgtgtgtagc ctgagggaca ttctttacac ggcagatcat gcggcagccg caagatcggc
     1861 atttctcctc cgatgcccca agtgccactg caaccgagat atacgcatca aacgagatct
     1921 cgaagctcgc gtgaggtatg gatggtcctg acccgaagcc ggaccacact gaccgttgcg
     1981 atggtcctac aaggactgca ggtgggtggg atggaacggt gcgccatcca gttagccaca
     2041 tctgcaatcg gtgcgggtta cgatgcgaga ctgattctat atgatgttcc cccaaggcgg
     2101 agcggagctg aatacgatcc gggcaatgtc ccagtcgcat tcgtttccag aaggcatggc
     2161 atcgacatta cgctgcctgt ccgcctcgct agaatattcc agtcgtggaa cgtcgacatc
     2221 gtacatgccc ggaataacgt tgctggtttt tattcggcag cagctctggg cctaattcaa
     2281 gatccccctc ccctcatttt gaccttcgac acgtttcccg ggcatgggac ggcgaaggct
     2341 cgcttagcga gcagatgggc gtcgcagtgc gccgcgatag tatcaagcgt gtccagcgac
     2401 ctgtcggatc gcctggtgag gagtggctgg gtcaatcgtt gcgagactgt ttggaacggg
     2461 gtcgacacgg gtgcattcaa tcccgacggc ccattacacg ggtttaagaa gagattcgga
     2521 tttcctcaac acaccctagt cattggaaac gtcgcacgac tggacgaaaa caagagacaa
     2581 ctcgatctga ttgaagcctt cagccgctta acaagagaaa acagcggcat agcattagcg
     2641 atcgcaggag acggaccaaa cagagaacgg gtcgtgaagg ctgccagtag tgtaaggaac
     2701 gtgctgatgc tcggaagaat ttacgatgtt gcagcattcc tgcgtgagat agacactttt
     2761 gtactttgtt ctgaagacga aggatgccct cgcgcactgt tggaggcgat ggcatgtcga
     2821 aagccgatca tcgcgactgc ggtgggcggg attcctcaca tcctcgcggg gtgcggtcta
     2881 ctcgtgcccc cacgcagacc tgacatgctg gcagacgcca taaatgtgat gtgcagttcg
     2941 agggaggtcc gtgagcaatt tggtcagttg gcccgcgcaa ggattctaga ctcattcact
     3001 ctggaacaag aatggtgtca ctacgagcga atgtatgaat ctgtaattca actacccact
     3061 aatagcttgg ccgttggacg attggcgaga cccgactgat atttagcgcg atatcggatt
     3121 cccgatgtga cgggtttggg gtcgcgtctg tgtcaactgg cccgtagtgc acgaaagcga
     3181 cgttttagag gcgcataagc attaacccac attttctttt tgaagttggc ctaaaccacc
     3241 gcacactacg gcagcacgaa ggggtcgaaa gaaaaccatg atgcaagaat cttccgggcg
     3301 ctgtgataac acatgaggac cgattggcgg tgcggtgcga tgcgcttgtg aactcgtcgg
     3361 cgaaagcatc cccctgcact cggaggcgcg agagttctgt cgcagggact gcgaaccggt
     3421 ttttccattc agtcgcacgg aaatcctgtt tttggtcgta tcgaacgtct gcagccacac
     3481 agcgaatcgc cgcggcagca caaccgcttg tctataatgg attttcacga cgcatcgcca
     3541 tcgttggagc ggcaaaccgg ccgtcgaggc gtatttggcg acggtcgcga cggattcctt
     3601 catgattcaa accttatttg gcagcaccga agaaaagccc agtttcctcc aacgcatgaa
     3661 agaggcggtc acgcggacgc gcgagaccct tagcgagc
//
LOCUS       DASRKK010000129         8606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_496973, whole genome shotgun sequence.
ACCESSION   DASRKK010000129 DASRKK010000000
VERSION     DASRKK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496973"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFC15_02945"
     CDS             <1..907
                     /locus_tag="VFC15_02945"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZM09150.1"
                     /translation="GVTVYSVYGDSDAKRRPSPDVIRNVDALVFDVQDVGVRFYTYEA
                     TLGYFLEAAAKAGKPIVVLDRPIPQNGAYVQGPVSDTGQASFVNYHNVAVRHGMTIGE
                     LAKLYNGERRITANLTVVPMKGWMRGDWFDATGAEWVNPSPNLRNLEQAILYPGVALI
                     EGTNVSVGRGTDSPFQVIGAPWIKPKQLSAYLNARLISGVRFVPTEFTPSASTYAGQA
                     CGGVSIVLTNRETLDAPELGIELAAALNKLYSQQFELMKMNALLADQVTFRGLQDGED
                     PRRLAEDWQTGLDRFMLVRARYLIY"
     gene            complement(943..1647)
                     /locus_tag="VFC15_02950"
     CDS             complement(943..1647)
                     /locus_tag="VFC15_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09151.1"
                     /translation="MRLKALLLVIVLSHALASLAQDQPQLVPRTPIAQQQSNVDPSAE
                     YSIPAGTKVPLTLTQGVTSKTAKEGDPVYAQTAFPITQNNRIVIPAGSYVQGVVRRVV
                     RPGRVKGRAELQMSFTSMIFPNGYTVLLPATVQGVPGSQDVDTKGSEGTIQGQGSKGK
                     DAATIAKTAGAGAGIGAITGGGKGAGIGAAAGSALGIATVLLTRGPEIQLNPGSSVEM
                     ILERDLTLEGTMLRQQ"
     gene            complement(1763..2509)
                     /locus_tag="VFC15_02955"
     CDS             complement(1763..2509)
                     /locus_tag="VFC15_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZM09152.1"
                     /translation="MKRIQPLLIAIPLLACILVGLTPHSLRAQERTSFASPNTVWVGA
                     DGKYEAEPDTALMQFDISAQEDNLQAANARATKAAEQVRQLLRNNNLDPKQAQVGMFS
                     VQPVYDYKNPKRKLVGYRVDTSITIKVKDFSKIGPITQGLADMDITGNQSLSYVLDDM
                     DAAKIKAVEDALHRAQEEASAVARSSGRTLGELSYASVDTYEQPRPRPMMMAAPMAMK
                     SDNAPPAPTEGFGAQKVSVTAHVNAMYNLK"
     gene            2800..3030
                     /locus_tag="VFC15_02960"
     CDS             2800..3030
                     /locus_tag="VFC15_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09153.1"
                     /translation="MRKWFVPLTVLGVGGVGAFLLTDKGRETLRRWRARFDEAPERWL
                     EWNENAQLELECIQAALNQIAKSLGPRSEMGR"
     gene            complement(3071..3358)
                     /locus_tag="VFC15_02965"
     CDS             complement(3071..3358)
                     /locus_tag="VFC15_02965"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZM09154.1"
                     /translation="MSAVLSDSEIDLALQKLPGWARHASTVERVFQFDDFVQAMKFVN
                     QIADAAEAANHHPDILINYNKVTLRLVSHDSGGVTQRDIRMAGKINEVAGG"
     gene            complement(3472..4575)
                     /locus_tag="VFC15_02970"
     CDS             complement(3472..4575)
                     /locus_tag="VFC15_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM09155.1"
                     /translation="MPREINPIQPKLEERILVVDDDESILEIISSMLTHAGYECRTVA
                     SGPEAIAALKADDSYALLLSDLAMEGMDGFGILERVKRLYPQLPVVMVTAVHDISVAL
                     AAIRGGAYDYLLKPFEREQLIATVRRALENRRLKLENISYQTNLEALVSARTELLERA
                     MTDLERSYDITLEALGDALDLKDAETEGHSKRVTAFTMAIARAMELPHDRVRVIARGA
                     FLHDVGKMAVPDAILRKPGRLTPEEQMLMREHALLGYQMLRKIPFLQEAAHIVWSHQE
                     RYDGSGYPRGLKGNQIPLGARIFAVADTFDAITSDRPYRAAQSISAARREIQKYAGLQ
                     FDPAVVETFISISEELWQKLRNEIDAQSSSKRR"
     gene            complement(5121..5654)
                     /locus_tag="VFC15_02975"
     CDS             complement(5121..5654)
                     /locus_tag="VFC15_02975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM09156.1"
                     /translation="MRSRTVWLIALVAVLAVAIGVVNRRWAPVHKVGAGSGGESKLAP
                     DFSLTDLDGRKLNLADYKGKVVLLDFWATWCGPCRTEIPHFVEMQNKYGTNGFQVIGI
                     SMDDDAKPVREFYQQYKLNYPVAVGDDKLAERYGGVLGLPVNFIIDRDGRIYARHLGA
                     TDVSVFDQEVQDLLAKH"
     gene            complement(5694..6221)
                     /locus_tag="VFC15_02980"
     CDS             complement(5694..6221)
                     /locus_tag="VFC15_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZM09157.1"
                     /translation="MNSSYRNQFFIAIYFAALVLLPAANAQAPVLFDGTGPVVFDFQA
                     AGSADSIPRSQLITPEDLAKSLTAATTPKPLLLGVGPRSLYAQAHIPGSEYMGAGSSD
                     GGRKQLRHRVKALPHNTAIVLYCGCCPWDHCPNVQPAYDELRVLGFSNVKVLFIANNF
                     GTDWVDKGYPVAKGE"
     gene            complement(6243..7814)
                     /gene="lysS"
                     /locus_tag="VFC15_02985"
     CDS             complement(6243..7814)
                     /gene="lysS"
                     /locus_tag="VFC15_02985"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZM09158.1"
                     /translation="MALDQKIYELRKEKLKQIEALGQQSYPYRYESTHTLPQILDEYT
                     PKTAEELTETRIDVSVAGRIMSIRLQGKAGFAHLQQGGKKLQIYVKLDFVGEKGFQLY
                     KLLDLGDYIGVKGYLFRTRTGELTVHVEEITFLVKDLLPLPEKWHGLQDVETRYRRRY
                     VDLTMNPEVREVFIKRAKVVQSMRRFLDGNGFVEVETPMMHSIAGGAVAKPFTTHHNA
                     LDLDLFLRIAPELYLKRLVVGGMDRVYEINRNFRNEGISTQHNPEFTMLEFYMAYADY
                     MDMMDFTEQMLGQVARDVNGTTIANFSGHEIDFGKFERLSMRHAIIKYWPENAGAKPE
                     MADFSSADRIAAMVRRYNAANPHIPYDPAAPNGKTIAEIFEFVAEPHLIQPTFIYDFP
                     LAISPLSKNKREPEENADWVERFEIFVGGLEIGNAFSELNDPEEQRRRFEQQLTERAR
                     GDEEAHQMDEDYIRALSYGLPPTAGEGVGIDRLTMLLTDSRSIRDVILFPLLRPLKRE
                     EEEALDAPDEGATTT"
     gene            8312..>8606
                     /locus_tag="VFC15_02990"
     CDS             8312..>8606
                     /locus_tag="VFC15_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09159.1"
                     /translation="MGTSGAASGLGAKGFGGQIHGPKHHQGGVRVDALGSENAVELDL
                     VPGKVAKDFGDAEAGDDGAATGAGHVVEACLGVEVMATAGAAVDGGLLTAASAG"
BASE COUNT         1753 a         2380 c         2589 g         1884 t
ORIGIN      
        1 gggcgttact gtctatagcg tgtatggtga cagcgatgca aaacggcgtc caagtccgga
       61 cgtgattcgc aatgtggatg cgctggtatt cgatgtgcag gacgtgggag tccgcttcta
      121 cacctacgag gccacgctgg gttatttcct ggaagccgca gcgaaggccg ggaagccaat
      181 cgtcgtgctg gaccggccga ttccgcagaa tggagcgtac gtccagggac cggtgtctga
      241 tacaggacaa gcgagctttg tgaactacca caatgtggca gtccgacatg gtatgaccat
      301 cggagagtta gccaagcttt ataacggcga gcgccgcatc accgccaatc tgacagtcgt
      361 accgatgaag gggtggatgc gcggtgactg gttcgacgcc accggcgcgg aatgggtaaa
      421 tccgtcaccc aatctacgta acttagagca ggcaattctt tatcctgggg tcgccctgat
      481 tgaaggcacg aacgtttcgg tcggacgcgg aacagactca ccctttcaag tgatcggtgc
      541 tccttggata aaaccgaagc aactctccgc ctatttgaat gcgagactga tctcgggtgt
      601 tcggtttgtg ccgacggaat tcactccgtc ggcgagcacc tacgctgggc aggcgtgcgg
      661 tggagtgagc atcgtcctga ctaatcgtga gacgctggat gcgccggaac tcggcataga
      721 actggctgca gctctgaaca agttgtattc gcagcagttt gaactcatga agatgaatgc
      781 attgctcgcc gaccaggtta ctttccgggg actgcaggat ggcgaagatc cgcgccgcct
      841 agccgaagat tggcagaccg ggctggaccg cttcatgctg gtgagagcga gatacttgat
      901 ctactgattt cggcgctcgt tgctcagcac cgggtagagg ctctattgtt gccgcagcat
      961 ggtgccttcc aaggtcagat cacgctccaa aatcatctcg accgacgatc cggggttcag
     1021 ttgaatttcg ggcccacgag tcaacagcac cgtggcgata cccagcgcgc tgcctgctgc
     1081 tgcgccaatc cccgcgcctt tcccgccgcc tgttatcgca ccgatgcctg ctcctgcgcc
     1141 agcagttttc gcgatggtgg cggcgtcttt gcccttgctg ccctgccctt gaatggtacc
     1201 ttccgacccc ttggtgtcaa catcttgaga accaggaaca ccctggacgg tggctggcag
     1261 cagcaccgta tatccgttgg gaaaaatcat cgacgtgaag ctcatctgca actcggcacg
     1321 gcccttgacc cgccccggtc gcaccacgcg ccgcaccacg ccttggacgt agcttcccgc
     1381 ggggatcacg atgcggttat tctgggtgat cgggaaggct gtttgcgcat aaaccgggtc
     1441 gccttctttc gcggtcttgg aggtaacgcc ctgggtgagc gtcagcggaa ccttggtacc
     1501 tgctgggatg ctgtattccg cggatggatc gacgttcgac tgttgctggg cgattggagt
     1561 tcgcggcacc aactggggct ggtcctgcgc aagcgaggct agtgcgtggc tcagaacgat
     1621 aaccaacaac aatgccttaa gccgcatggt tctcccgaaa cagaggttcg tagactactt
     1681 ctttaacgcc aagcgacaca caaagtcgcg gtgaattcaa tgacaggcac agcgttcgaa
     1741 ctcgctacct tcaggccagg atctacttca ggttgtacat cgcattcacg tgggccgtca
     1801 ccgagacctt ctgtgcacca aaaccctccg ttggcgccgg tggagcgtta tccgacttca
     1861 tggccatcgg agccgccatc atcataggcc tcggccgcgg ctgttcatac gtgtccaccg
     1921 aagcgtagga cagttcgccc aaggtgcggc cactgctgcg ggcgacggcg cttgcttcct
     1981 cttgggcacg gtgtaacgcg tcttcgacgg ccttaatttt ggcagcgtcc atatcatcga
     2041 ggacgtagct caaagattga ttgccggtga tatccatatc ggcgaggccc tgggtgattg
     2101 gcccgatctt ggaaaagtct ttaaccttga tggtgatgct ggtatcaacc cggtagccaa
     2161 ccaacttgcg cttaggattc ttgtagtcat agaccggctg taccgaaaac attcccacct
     2221 gcgcctgctt gggatcgagg ttgttattgc gcaggagctg gcggacttgt tccgcggcct
     2281 tggtggcgcg ggcgttggcg gcctgcaggt tgtcttcctg ggccgagatg tcgaattgca
     2341 tcaatgcggt atcaggctcg gcttcgtact ttccatcagc gccgacccaa acggtgttgg
     2401 gagacgcgaa cgacgttcgc tcctgtgcgc gcaacgaatg cggcgtcagg ccgacgagga
     2461 tgcaagcgag aagtgggata gcaatcagca aaggctggat tcgcttcata gtgcctttca
     2521 gacgcgaaga atcgcacaag ctcaaccata cacattgtaa gtcgaggcgg cgtggccagc
     2581 acgcaacttg ctgtgtgact cactatctga atgctgcgtt atgccgctaa gaccgaccac
     2641 caatcgcctg gacttagttt cgtttcggga aatctgccgg aatcaagctc gataccgaca
     2701 gtttgcatcc tgtgaagcat tcaaatcgtg gagaatagga tcatagaggc gagtcaccgc
     2761 gagattcctg agccggcgac tattcccaag gagcggccca tgcgcaaatg gtttgttcca
     2821 ctcactgttc tcggcgtcgg tggtgtcggg gcctttctgc ttaccgataa aggcagagaa
     2881 accttgcgac gctggcgggc aaggttcgac gaagcgcccg aacgttggtt ggagtggaac
     2941 gagaatgctc aactggaact ggagtgcatc caggctgctc taaaccagat tgccaaatcg
     3001 ctcgggcccc gaagcgaaat gggacgctga ttccatccct ggctttcaac gcaatttccc
     3061 ggatcgtgct ctaaccgccc gcaacttcgt tgattttccc cgccatccga atgtcgcgct
     3121 gggtgactcc gccagagtcg tgagacacaa gcctgagggt gaccttgttg taattgatga
     3181 gaatgtcggg atgatgattg gcggcttctg cagcatcagc gatctggttg acgaatttca
     3241 tggcctggac aaagtcgtcg aattggaaca cacgttcaac ggtgctcgcg tgtctggccc
     3301 atcccggcaa cttttgcagc gcaaggtcaa tctcggaatc ggataggaca gcgctcatgc
     3361 gaactcgcct cctgattggc tggaagctag ctagtgtatt tcgacgggcc gcaatcgagt
     3421 caagttatcg gtgtgtcgag tcacttcgcg gcagacagtg tcgcgccagg tttaccgtct
     3481 tttcgatgaa ctctgggcat cgatttcatt acgcaatttc tgccacaatt cctccgagat
     3541 tgaaatgaag gtttccacca ccgccggatc gaactggagc cccgcatatt tctggatctc
     3601 gcggcgggcg gcggaaatgg actgcgcagc gcgatacggc cggtctgacg taatggcgtc
     3661 aaaggtgtcg gcgaccgcaa atattcgcgc tccaagggga atctggtttc ccttcagacc
     3721 gcggggatag ccgctgccgt cataccgttc ctggtgggac cacacaatgt gcgcggcctc
     3781 ctgcaggaag ggaatcttgc gcagcatctg ataacccaga agcgcatgct cgcgcattag
     3841 catctgctct tccggggtga ggcgtccagg cttgcgcagg atggcatccg gtacggccat
     3901 ctttcccacg tcgtgcagga aggccccgcg cgcaatcact cggacgcgat catgcggcag
     3961 ttccatggcc cgtgcaatcg ccatggtaaa ggcggtcaca cgtttggagt gcccttcggt
     4021 ttcggcatcc ttcaaatcga gcgcgtcgcc cagcgcctca agagtgatgt cgtaagaccg
     4081 ctcaagatcg gtcatggctc gttccagcaa ctccgtacgg gcggatacga gcgcttccag
     4141 gttggtctga taggagatgt tttcaagttt caggcggcga ttctccaggg cacgtcgcac
     4201 cgtcgcgatg agttgctcgc gctcgaaggg tttcagtaga tagtcgtagg cgccgccgcg
     4261 aatggcggcc aaggcaactg aaatgtcgtg aaccgccgtg accatcacca ccggcaattg
     4321 aggatacagc cgcttaacgc gctccaatat cccgaagcca tccatgccct ccatggccag
     4381 atcggagagc agcagggcat agctgtcatc tgctttaagc gcggcaatcg cctccggacc
     4441 ggaagcgacg gtacggcatt cataaccggc gtgagtcagc atcgaggaga tgatctccaa
     4501 tatggactca tcatcgtcga cgaccaggat gcgttcctca agcttgggct gaatcggatt
     4561 gatttcccgg ggcaagtatc accagccagc gacgattgta accacgaatg acggatttgc
     4621 tacgtttttc gtgctgagtt gccataaaaa ttcggtgctt agtgtcgaaa tagtaaataa
     4681 gacatttgtg ggcaaccgga ttttagaacg accggtctct cgccctttcg tcgcggcgtt
     4741 ttcgagtttg atcggatcac ctcattgtga cggcttcgcc gattgaaatc tggagacaga
     4801 aagttgcacg cgatgcgcgc cgctatttgg tgcgtgaagt tggcagttcc gaagctggtt
     4861 gggtgcgatc actccgtcga gactcgacgg aacaggattt gctccgtcaa gtttgcacgc
     4921 ccagcttggg cagtaaccat tgtgtcagca caatgtagcc gactacgaag aagacaatcc
     4981 aagcccagtc tggcaaattc tctcctcagc aacagttcga cgaacggaag atctggactg
     5041 caaccactag gttacacgct gaagtccaat ccctgccagc gaatcacaac atcgattccg
     5101 tgagaactca aggtcgtgcg tcagtgcttc gccagtaagt cctgcacttc ctggtcgaat
     5161 accgagacat ccgtagcccc aagatgcctg gcgtaaatgc ggccgtcgcg atcgatgatg
     5221 aagttaaccg gcaacccgag tacaccgcca taacgctctg cgagtttgtc gtcacccacg
     5281 gcgacgggat agttcaactt gtactgctga tagaactcgc gcaccggctt ggcatcgtcg
     5341 tccatcgaga tgccgatgac ctggaagccg ttcgtgccgt acttgttctg catctccaca
     5401 aagtggggaa tctcagtgcg acagggcccg caccaggtgg cccagaaatc gagcagcacc
     5461 accttgccct tatagtcggc gagattaagc tttcggccat caaggtcggt gagagaaaag
     5521 tctggagcga gtttggattc gccaccggag cccgcgccta ctttatgaac gggcgcccat
     5581 cgccgattga cgacgccgat tgccacggcg agcacagcca ccagcgcgat cagccatact
     5641 gttctacttc gcatgaaggc ggagtctcct tgaagttgtc acgtgcagcg agcttattcg
     5701 cctttggcga ccggatagcc tttgtccacc cagtcggtgc cgaaattgtt ggcgatgaag
     5761 agcaccttca cattgctgaa accgagcacg cgcagttcgt cgtaagcggg ctgaacattg
     5821 gggcagtgat cccacgggca gcatccgcag taaagcacga tggcagtgtt atgcggcagt
     5881 gctttgacac ggtggcgcaa ctgtttgcgc cccccgtcgc tagatcctgc gcccatgtac
     5941 tccgaaccgg gaatatgagc ctgcgcatag agcgagcgcg gtcccacgcc cagcagcagc
     6001 ggcttgggtg tcgtcgctgc cgtcagcgac ttggcgaggt cttcgggagt gatcagctgg
     6061 gagcggggaa tggagtcggc agaccccgcg gcctgaaagt cgaagaccac gggaccggtg
     6121 ccatcgaaca agaccggcgc ttgcgcgttg gcggcaggca aaagtaccag cgccgcaaag
     6181 taaatggcga tgaagaattg gttacgataa gaactgttca catggtccct tctgtatttg
     6241 aatcaggttg tggtggcgcc ctcgtccggc gcgtcgagcg cttcttcttc ctcgcgcttt
     6301 agcgggcgta gaagagggaa gagaatcaca tcgcgaatgg agcgcgaatc cgtgagcagc
     6361 atggtgaggc gatcgatgcc cacgccttcg cccgcggtcg gtggcaggcc gtaagacaga
     6421 gcgcgaatgt agtcttcgtc catctgatgg gcttcttcgt cgccgcgagc gcgttcggtg
     6481 agttgttgtt caaagcgtcg gcgctgctct tcgggatcgt tgagctcgct gaaggcgttg
     6541 ccgatctcca ggccgccgac gaagatctcg aagcgctcga cccagtcggc attctcctcg
     6601 ggctcgcgct tgttttttga aagcggcgag atggcgagag ggaaatcgta aataaacgtc
     6661 ggctgaatga ggtgtggttc cgccacgaat tcgaagatct cggcgatggt cttgccgttg
     6721 ggcgcggccg gatcataggg gatgtgagga ttggccgcgt tgtaacgcct taccatcgcg
     6781 gcgatcctgt cggcactgga gaagtcggcc atctccggct tcgcgccggc gttttcgggc
     6841 cagtacttga tgatggcgtg gcgcatcgac aggcgctcga acttgccgaa gtcgatttca
     6901 tgcccgctaa agttggcgat ggtggtgccg ttcacgtcgc gcgcgacttg cccgagcatc
     6961 tgctcggtga agtccatcat gtccatgtaa tcggcatagg ccatgtagaa ctcgagcatg
     7021 gtgaactcgg gattatgctg cgtcgagatg ccttcgttgc ggaaattgcg attgatctcg
     7081 tacacccggt ccatgccgcc aaccaccagg cgcttgaggt aaagttcggg cgcgatccgc
     7141 aggaacaggt ccagatcgag cgcgttgtga tgcgtggtga agggcttggc cacagcgccg
     7201 ccggcgatgg agtgcatcat cggggtctcg acctccacaa atccgttgcc atcgaggaag
     7261 cggcgcatcg actgcaccac cttggcgcgc ttgatgaaga cctcgcgcac ctcgggattc
     7321 atggtgagat cgacatagcg gcggcgatag cgggtttcga cgtcctgcag gccgtgccat
     7381 ttctccggca aggggagcag gtctttcacc aggaaggtga tttcttcgac gtggaccgtg
     7441 agttcaccgg tgcgggtgcg gaagaggtag cccttcaccc cgatgtaatc accgagatcg
     7501 agcaacttat agagctggaa gcccttttcg ccgacgaaat cgagcttgac atagatctgc
     7561 agcttcttgc cgccctgctg gagatgggcg aatccggcct tgccttgcag gcgaatcgac
     7621 atgatgcgcc cggcaacgct gacgtcgatg cgcgtctcag tcagttcttc ggcggttttt
     7681 ggtgtgtact cgtccagaat ctggggaaga gtgtgggtgg attcgtagcg gtagggataa
     7741 gactgctgtc ccaaggcttc aatctgcttg agcttttctt tgcgaagttc gtagattttc
     7801 tggtcgagtg ccaatgtgcg ttcctagagt gcaagtgaat tcttaattat agatgtcaag
     7861 agccattcac cacggagacg cggggacgct tggccctttt caggcgagcc gagtgccgcc
     7921 gggttaagac gctgttacga aatgctgccg tgcctgcggc actcccatga attctcccac
     7981 tcttcccagc actccgcctt cggctgcgtg ctgggctaga ctattccgcg cctccggcgc
     8041 tcgcattcgt agcgtttgtt ccaccccaca aaacgaagcg cgagtttagt aacaggctcg
     8101 ttaaaacccg gctcccttca aaagtccagg ctggacgcgg cgctgaagcg ccgctctacc
     8161 gagccgggtg tgcgagttcg ccggattgtt cactccctcc tccctgcctc tttgtgtttt
     8221 caatcatttg cgagagggtt gcgctcaagc tttgtggacg caaagggttt agtgccacat
     8281 cacagattct attagggtta gaagcgcggt tttgggcacg tcgggggcgg caagcggact
     8341 cggcgcgaag ggtttcggcg gtcagatcca tggcccaaag catcatcagg gcggcgtcag
     8401 ggtcgatgcc ctcggtagcg agaacgcggt tgagctcgat cttgttccag gcaaggtcgc
     8461 gaaggatttc ggggacgcgg aagcggggga tgacggtgcg gcgacgggcg cgggccatgt
     8521 tgtggaggcg tgcctgggcg tcgaggtgat ggcgacagca ggcgcggcgg tggacggcgg
     8581 gctgctgaca gcggcgtccg ccgggg
//
LOCUS       DASRKK010000130         8981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_594712, whole genome shotgun sequence.
ACCESSION   DASRKK010000130 DASRKK010000000
VERSION     DASRKK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594712"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..2151
                     /locus_tag="VFC15_02995"
     CDS             475..2151
                     /locus_tag="VFC15_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09160.1"
                     /translation="MAKSSSVKLALVATMLVLCGFVGCLANFTLPLLSPYVSRVGPPR
                     IERWRLRALLIWPTVPFLLGLMRKLWLGDHLTATNQYANILRSAYLRSGVSPLLPQVL
                     LLLGMYAWFWFNLQGLALFGADRPVLPRRRQLPTVDIPTRQRHGHTVNAHETAAFRMF
                     SQDDAGVDIEDTALPLTAYYGISLLVFLFLTILISQLAIGEFSLRTLGDRRFGELIFF
                     WICLFIAMILADTLQFSRTWSRLRRLLIFMDRLRLRRTLEALKGLSWDSVWKMSGNVL
                     EQRYRLISRQFESMSNLSNILHAWQPAEDQEKTARSIALQQLKRCGHSGTLFADWYVN
                     LGHSSRATDLTDLRNFQLELAAAAGCVMTTIILPAWHKETQSLILDSTISTDTSKWDE
                     PPDQPGDDKDGNLTEAGHKTPADVAASERKTVVQAAEEFFVLPYLGFIQNTIGRIRSI
                     ALSILTLFVAATLAISSYPFDPLPTIGALFLVIFLLAGVVVVYVYAEMHRDSTLSLIT
                     NKTPGELGLDFWMKLIAFGVGPLIGLLTTLFPSMTDFVVSFLQPGAQAIK"
     gene            2243..3073
                     /locus_tag="VFC15_03000"
     CDS             2243..3073
                     /locus_tag="VFC15_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09161.1"
                     /translation="MRGVVSFVACGYLLCIGAAAAETGKLLPTASLAKQVVASGKLLS
                     DSLPGTTMESRRKVGFAVRDRISQFIVDQIEAKPDITKNQLTTQLRAILCSGEYSMCD
                     ISQQPAVLSTGWFGPNGKGQFAVAYQLNLGFMGPKGAITVVESYWVEYAKTVKHWAHG
                     GSEFDAYTANFQLIQQFIDPNEIWILAWGTVLGGNGRGISGRATLYRLGGDEVKVAWD
                     DAREVNVSAQKSLIGWEFSYADKQMLYGNDPKPYFFDVYQLNYLDRSFSRVAHYRYAD
                     "
     gene            complement(3142..3735)
                     /locus_tag="VFC15_03005"
     CDS             complement(3142..3735)
                     /locus_tag="VFC15_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09162.1"
                     /translation="MHHNLTANLSEFRYHYLMADLPILDSSSPRSGREELAMAGFRVD
                     PTHEGPQFYTLLAVGGDNERPLVADGRIVFFVRTTLVHKALAMDESLAVLGNPPRDVE
                     TICDVAQTLYLVNSQDADPDGVVLDCLLIFDDLVRASGISMPERYQGILTELAARLTE
                     GQSLKKVFTSEALRDHVEDALLWCVGAIAMKAKILTS"
     gene            3989..4588
                     /locus_tag="VFC15_03010"
     CDS             3989..4588
                     /locus_tag="VFC15_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM09163.1"
                     /translation="MDDNQAVNALVRRCVAGDAAAWQEIVQQYHRRIYNICYRFSGSP
                     DDAADLTQDVFIKVYRTLGTFDGTRAAFMTWVTTVTRNLLVDHFRKGKYDRVTDSLEA
                     TPGGQEDGLTVGEQLPDQSASPEEQMQSKQTQKMVHEALQKLSPELREAVILRDLQDM
                     DYKEIALTLKVPEGTVKSRINRGRTELARLLQRTYRQVN"
     gene            4588..5325
                     /locus_tag="VFC15_03015"
     CDS             4588..5325
                     /locus_tag="VFC15_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM09164.1"
                     /translation="MDGESKSGVQCVEFEALLAEALDATLHGATLAAFEAHQKSCPAC
                     AAMYQEAAAGMHWMKGLEDIEPPQNLVHNILAQTIGTVAEANIKPAPVGESWLDRLKS
                     KISPVFAPVVTPRFAMSFGMAFFSITMLANIAGLHMSDLKHVDLSAKGIQKTYYSTQA
                     RAVRYYENIRLVYEIESRVRDLRRSATPEKQEEPSTPTNPKKGAAQEQNRKYQNYSRE
                     ANQPMLARCPAVDEQQTLQARLNWRTI"
     gene            5322..6044
                     /locus_tag="VFC15_03020"
     CDS             5322..6044
                     /locus_tag="VFC15_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZM09165.1"
                     /translation="MNCANHVETPAVAYCRTCGKPMCSTCTRDVRGVVYCEECLASQM
                     SGTVPPPPVPGTVLPGAPPPVSGNSPGLAAILGFIPGVGAMYNGEYTKGFIHVLIFAS
                     LIWATDHVNDLFGLGIAAFVLYMPIEAYQTAKAKMMGVRAPDPFGLNNLNLFGSANPP
                     AAAPGVAAGTPVEGQPLPVEEEYRGGSRIPVGAFVLIGLGVIFLLEQFGWFHFHWFGK
                     FWPVILIAVGIRVLMKRTGRGW"
     gene            6055..6426
                     /locus_tag="VFC15_03025"
     CDS             6055..6426
                     /locus_tag="VFC15_03025"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZM09166.1"
                     /translation="MRYRYNPSCGCPRCRAHGFMGPAVLITLGVLFLLDQVVHVYWME
                     FGRTWPALLIVIGLVMFLQHNAPATGHVPREYIGPPPVQPTAPWQPPQPQQWHPPVVT
                     PPQAPEAGPFTGTDKPEVPNG"
     gene            6419..7819
                     /locus_tag="VFC15_03030"
     CDS             6419..7819
                     /locus_tag="VFC15_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM09167.1"
                     /translation="MASQYQPAPHLPPAPPTYRYRRSLAGPLILVLIGLVFLLRNFGV
                     HIPVWHFFGRFWPVLIILWGVIALVEHFTALKNGYQTRHLGGLGIMLLILIVIVGLAA
                     HHSSDVNWEGMRDQMQIDDDLGGMFGSAFTFDDTMEQQFPAKASLRVVCDRGAINVTP
                     SDGNTIRVVVHKKLYANNQEDANKYNEGSKPQITVNGTQVLLNGNTNGAGDHAVQTDM
                     DLFVPRDASVDVASKRGDVSITDRKADVKLTLQKGDITLSDVTGSAKMSMDKGSIRAS
                     NVTGDVAIDGRVDNVTLEDIKGAVQLNGDFFNDIRLSKITRTVEFKSARSDISLASVP
                     GDLEISGDSLRATDVSGPMRFITRSKDIHLEGVTGDLQVENANGDIEIHPASKLPIGK
                     MMLTGKHGDITVVLPANAGFQVDATTRKGDITSDFSPLRNEETNGSSHTTGTIGNGAT
                     KLQINADTGDIRINKG"
     gene            complement(7886..8566)
                     /gene="cmk"
                     /locus_tag="VFC15_03035"
     CDS             complement(7886..8566)
                     /gene="cmk"
                     /locus_tag="VFC15_03035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZM09168.1"
                     /translation="MSAARKLVIAIDGPAGSGKSTIAARLAKKLGYINLETGAMYRAL
                     ALKALEQQVSLDDPEVLRRLAAGTVIELEPQAEGNRVLLDGRDVSQRIRQSDVTLAAS
                     RVSMHPPVREIMVSRQREMGASGGVVMEGRDIGTAVFPDADVKVFLDADARVRAERRV
                     AQNGTLAAEEAQRIVEDLVARDQRDRTRAVSPLVPAADAVIIDSTNLAIDDVVSRVEQ
                     LANSKLET"
     gene            complement(8563..>8981)
                     /locus_tag="VFC15_03040"
     CDS             complement(8563..>8981)
                     /locus_tag="VFC15_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09169.1"
                     /translation="FWDVLFRWSPEAVKEGMHLSVPESLTALVSKYIDCVQAVPQDEV
                     ESFFSPLVGRAKVRDAINALQAGRELGFVHVGGRVMLQAAEARLPHKARERRTEPWSE
                     ENPPELRPGKPLVEHRPRRKIRPSRPAPPRQDNPKE"
BASE COUNT         1877 a         2647 c         2663 g         1794 t
ORIGIN      
        1 atgcgccact gcgagcaacc agtcccgagc atctcaagaa tccggatgaa gaaaaccagc
       61 cggcaacctg ggtttccgtt gtgggacacc ggcagttctg gcccctagct gtgctgaact
      121 ccaacaccga ggttaactcc aaaggtgagg cgtatgggcc cgtcaccgag ccccacactc
      181 atgacgagtc cctgctgact cacgaggacg ggggtgataa gtttgaccca aatcgccaag
      241 ccgacagtcg cttgccgggc gaactcgtcg gactgctcct gctgagcctc gcactgggat
      301 tctgtcacct gtacttctgc tggaaagggt ccataatcgg cacgccccga gtgcgagcct
      361 acttcgcccc ggttccgagg atgcaacacc tcgtacttat ctccctcggc agtctctttg
      421 tcgggtattt ggggggtaac gatcgcacta acgttgggga gaggcagcga atttttggcg
      481 aaaagttcgt ctgtaaaact cgcgctcgta gcgaccatgc tggtcctttg tgggttcgtg
      541 ggatgtctgg cgaactttac tcttccgctg ctctcgccgt atgtatcgag agtcggtccg
      601 cctcgtatcg aacgttggcg actgcgcgcc ctgctcattt ggccgactgt acctttcctg
      661 ctcggcctga tgcgcaaatt gtggctaggt gaccacctga ccgcgaccaa ccagtacgcg
      721 aacattctgc gcagcgctta tttacgcagt ggagtgtcgc cgcttctgcc gcaggttctg
      781 ctgcttctag gtatgtatgc ctggttctgg ttcaacctgc aaggactggc gcttttcgga
      841 gcggaccgcc ccgtattgcc ccgacgaaga caactgccca cggtcgacat acccactcga
      901 cagcgccacg gacatacggt gaatgcccat gaaactgcgg ccttccgtat gttcagccag
      961 gacgatgccg gggtggacat tgaagacaca gccctgccct tgaccgccta ctacggcatt
     1021 tcactgctgg tgtttctgtt cctcacgatc ctgatctccc agctggcgat tggcgagttc
     1081 agcttgcgga ccctgggcga ccgccgcttc ggcgaactga tttttttctg gatatgtctc
     1141 ttcatcgcca tgattcttgc cgacactctg caattttcac ggacttggag ccggttgcgc
     1201 cggcttctga ttttcatgga tcgtctgcgc cttcgacgca ccctcgaagc gcttaaggga
     1261 ctttcctggg attctgtctg gaagatgagc ggcaatgtcc tggagcagcg ctaccggctg
     1321 atatcgcgtc agttcgaaag catgagtaac ttgagcaaca tccttcacgc ctggcagcca
     1381 gcagaggacc aggagaaaac ggcccggtct atcgccttgc agcagcttaa gcgctgtgga
     1441 cacagtggca cgttgttcgc cgactggtac gtcaatctgg gccactcctc gcgcgccact
     1501 gacctgaccg acttgcggaa ttttcagctg gaactagccg cggctgcagg ctgcgtgatg
     1561 acgacgatca tcctgcccgc atggcacaaa gaaactcagt cgctaattct cgatagcacc
     1621 atatccacgg ataccagcaa gtgggacgag ccgcctgacc aaccaggcga cgacaaggac
     1681 ggcaatctca cggaagccgg ccacaaaaca cccgcggacg tcgccgccag tgaacgcaag
     1741 accgtggtgc aagctgcgga ggagttcttc gtgttgccct atctgggctt cattcagaac
     1801 acgattggca gaattcgctc cattgcgctg agtattctga ctcttttcgt ggctgcaacc
     1861 ctggccattt cgagctaccc attcgatccg ctaccgacga ttggcgcgct ttttctggtt
     1921 atcttcttgc tggccggagt ggtggtcgtt tacgtttatg cggaaatgca tcgcgactcg
     1981 accctcagcc tcatcaccaa caagacgccg ggagaactgg gcttagactt ctggatgaaa
     2041 ttgattgcct tcggcgtcgg cccactcatc gggttgctga ccaccttgtt cccgtcgatg
     2101 acggattttg tggtgtcgtt cctgcagccg ggagcacagg cgatcaagta gggcaagcat
     2161 tgagtttgtg aggtcaacgt taggcgggga gaacccgtgc cttctggatt gagctgtgag
     2221 attctgcagt acgaggcgct tcatgcgtgg ggtcgtgagc tttgtggcgt gcggttattt
     2281 actgtgcata ggcgcggccg ctgcggagac gggaaagctg ttgcccacgg catcgctggc
     2341 gaagcaggtg gtcgcgtcgg gcaagttgct gagcgactct ctgcccggca ctaccatgga
     2401 gagccgccgc aaggtcggct tcgccgtccg cgaccgcatc agccagttca tcgtcgacca
     2461 gatcgaagcc aagcccgaca tcaccaaaaa tcagctcaca acgcaattgc gcgcgatcct
     2521 ttgctcgggc gaatactcga tgtgtgatat cagccagcag cctgcggtgt tgagcaccgg
     2581 ttggttcggt cccaacggca agggccaatt cgccgtggcc taccagctaa acctcggatt
     2641 catggggccc aaaggcgcga tcacggttgt ggagagctat tgggtcgagt acgccaagac
     2701 cgtgaagcac tgggcccacg gtggcagcga gttcgacgcc tacaccgcca actttcagtt
     2761 gattcagcag ttcattgacc ccaacgaaat ctggatatta gcatggggaa ctgtactcgg
     2821 cggcaatggt cgcgggatta gcggacgagc aaccctatat cggctcggcg gggacgaggt
     2881 caaagtggca tgggacgacg cgcgcgaggt caacgtgtcg gcccaaaaga gcttgattgg
     2941 ctgggaattc agttacgcgg acaaacaaat gctctacggc aacgatccca agccctactt
     3001 cttcgacgtt tatcagctca actatctgga tcggtcgttc tcgcgggttg cgcactatcg
     3061 gtacgccgac tgatctacca ctggcacccg gcctgaattg aatctatgtg tctcagtgcc
     3121 tctgtggtga aagcctctgc ctcagctggt caggatcttg gccttcatcg cgatcgcgcc
     3181 gacgcaccac aacagtgcgt cttccacgtg gtcacgtagc gcttcgctgg tgaagacctt
     3241 cttcaaactt tgtccttccg tcaggcgtgc ggccagttcg gtcaggattc cctggtaacg
     3301 ctcgggcatg ctgatgccgg aggcgcgaac caggtcgtcg aagatcaaca ggcaatcgag
     3361 taccacgccg tcgggatctg cgtcctgcga gttgaccagg taaagcgtct gcgcaacatc
     3421 gcagatggtc tcgacatcgc gcggcggatt gcccagcacc gccagggatt cgtccatggc
     3481 cagcgccttg tgcaccagcg tggtcctgac gaaaaacacg atgcgcccat cagcgacgag
     3541 ggggcgctcg ttatcgccgc ccaccgccaa caatgtgtag aactgcgggc cttcgtgggt
     3601 agggtccacg cggaatccgg ccatggccag ttcttcgcgg cccgagcggg gcgaactgga
     3661 atcgagtatg ggcaggtcag ccataaggta atggtaccga aactcgctga gattggcagt
     3721 gagattgtga tgcatcggga accaaggtct tggcgaatca caatccgctc gtagaaaaag
     3781 agctcattgc gttcagaatg acagcagggt gcggcgatcg cgcgtcagat actcactagg
     3841 atttgggcgg gctgctacaa tttctttgcg aaaactcaag ggcggttcgc ccttgcagtc
     3901 aaacgaactc ctgacctcaa atttgcgtac aagtctttag ccctcataaa gatttgaggg
     3961 ccaggggtca gcccattggg agctctactt ggacgacaac caggcggtca atgcactggt
     4021 ccgacgctgt gtagcgggcg acgccgcggc atggcaggaa atcgtccagc agtaccatcg
     4081 gcgtatttat aacatctgct atcgcttctc gggctcgccc gacgacgcgg ctgacctgac
     4141 ccaggacgtg ttcatcaagg tttaccgcac gctgggcact ttcgacggga cccgggccgc
     4201 cttcatgaca tgggtcacca ccgtgacgcg caacctgctg gtggaccact tccggaaagg
     4261 caagtacgac cgggtcaccg attcgctgga agcgaccccg ggcggccagg aagacggcct
     4321 gacggtaggc gagcaactgc cagaccagtc ggccagcccg gaagaacaaa tgcagagcaa
     4381 gcagacccag aaaatggtgc atgaagccct gcaaaagttg tcccccgagc tccgcgaagc
     4441 agtgattcta cgggacttac aggacatgga ctacaaggaa atcgccttga cattaaaagt
     4501 tcctgaggga acggtaaaaa gccggattaa tcgtggacga acggaacttg cacgcctact
     4561 acaacgtact tataggcagg tgaattgatg gacggcgaat caaaatccgg agtgcagtgc
     4621 gtagagtttg aggccctgtt ggctgaagcg ctcgacgcaa cactccacgg cgcgaccctg
     4681 gcggccttcg aggcacatca gaaatcgtgt cccgcctgcg cggccatgta ccaggaagcc
     4741 gcggccggta tgcactggat gaagggtctt gaggacatcg agccacccca gaacttggtg
     4801 cataacattc tggcgcagac cataggcacg gtggcggaag cgaacatcaa gcccgctccc
     4861 gtgggtgaga gctggcttga ccggctgaag tcgaagatct ctccggtgtt cgccccggta
     4921 gtaacgccgc gctttgcgat gtcgttcggc atggcgttct tttccatcac catgctggcc
     4981 aatatcgcgg gcttgcacat gagtgacctg aagcacgttg atttgagcgc gaagggcatc
     5041 cagaagacgt actactccac acaagcgcga gccgtgcgtt attacgaaaa catccgactg
     5101 gtgtatgaga tcgagtctcg cgtgcgcgat ttgcgccgca gcgcgacgcc cgagaagcag
     5161 gaggaaccga gcacgccaac caatcccaag aagggtgctg cacaagagca gaaccgcaag
     5221 taccagaact acagccgcga agcgaaccag ccgatgctgg cccgctgtcc ggcggtagat
     5281 gagcaacaga ccctacaggc ccggctgaac tggagaacta tatgaactgc gccaaccatg
     5341 tcgaaactcc cgcagtcgca tactgccgca catgtggcaa gcctatgtgc agcacgtgca
     5401 cgcgagatgt gcgtggcgtc gtctactgcg aggagtgtct ggcctcgcaa atgagcggca
     5461 cggtgccgcc accaccggtc ccgggaaccg tacttccggg cgctccacct ccagtgagtg
     5521 gaaacagtcc cggcctcgcc gcaattctgg gcttcatccc gggcgtgggc gccatgtaca
     5581 acggcgaata caccaaggga ttcatccacg tactgatttt cgccagcctg atctgggcaa
     5641 cggaccacgt caacgatctg tttggactcg gtatcgcggc cttcgtgcta tacatgccga
     5701 tcgaggccta ccaaaccgcc aaggcgaaaa tgatgggcgt gcgtgctcct gatccgttcg
     5761 gtctgaataa cctgaacctg tttggaagcg caaaccctcc ggcggcagct ccaggtgttg
     5821 ccgcgggaac accagtggaa ggacagccgc tgccggtcga ggaagagtac cgcggaggtt
     5881 cgcgcattcc ggtgggagcg ttcgtcctga tcggtctggg cgtgatcttc ctgctggaac
     5941 agtttggctg gttccacttt cattggttcg gaaaattctg gcccgtcatc ctgatcgccg
     6001 tcggtattcg cgtactgatg aagcgtacag gccggggatg gtgaggagga cgtcatgaga
     6061 tatcgttaca acccaagttg tggatgtcct cgttgccggg cccacggatt catgggaccc
     6121 gctgtgctga taaccctcgg agtgttgttc ctgctggacc aggtggtcca cgtgtactgg
     6181 atggagtttg gcaggacctg gccggcgttg ctgatcgtca tcggactggt catgttcctg
     6241 caacacaatg ctccggcgac gggacacgtg ccgcgcgaat atataggacc tcctccggta
     6301 caaccgaccg cgccatggca accgccacag ccgcagcagt ggcatccgcc ggtggtaacg
     6361 ccaccgcagg ccccggaagc gggaccgttc acaggaaccg ataaaccgga ggtgcccaat
     6421 ggctagccaa tatcaacccg cgcctcactt gccccccgct ccgccaacgt atcgctaccg
     6481 gcggtcgttg gcaggcccac tcatcctggt cctgatcggg ctggtgttcc tgttgcgaaa
     6541 cttcggggtg cacattccgg tgtggcactt cttcggacgc ttctggccgg tgctgatcat
     6601 tttgtggggc gtgattgccc tcgtcgagca cttcaccgcg ctcaagaacg gctaccagac
     6661 gcgccacctc ggcggcctcg gcatcatgct gctgatcctg atcgtgatcg tgggactggc
     6721 ggctcaccac agctccgacg tgaactggga aggcatgcgc gaccagatgc agatcgacga
     6781 cgatctggga ggaatgttcg ggtcggcgtt taccttcgat gacacaatgg aacagcagtt
     6841 tccggcaaag gcaagcctgc gcgtggtgtg cgaccgcggc gcgatcaatg tgactccgtc
     6901 ggatggcaac accattcgtg tcgtcgtgca caagaagctg tacgccaata atcaggaaga
     6961 cgctaacaag tacaacgagg gcagcaagcc gcaaatcacc gtgaatggaa ctcaggttct
     7021 gctcaatggc aacaccaacg gcgcaggcga tcatgcggtc cagaccgata tggacctctt
     7081 cgtgccacgc gatgcctcgg tggatgtcgc cagcaagcgc ggcgatgtca gcatcactga
     7141 ccgcaaggcc gatgtgaagc tgactctgca gaaaggcgat atcaccctga gcgacgtcac
     7201 aggtagcgcg aaaatgagca tggataaagg ctcaatccgc gccagcaacg tgaccggcga
     7261 tgtggcgatc gacggccgcg ttgacaacgt gacgctggag gacattaagg gtgcggtgca
     7321 gttgaacggc gacttcttca atgacatccg cctcagcaag atcaccagga cggtggagtt
     7381 caagtcggcg cgttccgaca tttcgctggc atcggttccg ggcgacctgg aaatctccgg
     7441 cgactcgttg cgggccaccg acgtcagcgg accgatgcgc ttcattaccc ggtccaaaga
     7501 cattcatctg gaaggcgtga ccggcgactt gcaagtggaa aacgcgaatg gcgatattga
     7561 gatccatccg gccagcaagc tgcccatcgg caagatgatg ctgaccggca agcacggcga
     7621 catcactgtc gtactgcctg ccaacgccgg attccaggtc gatgccacca cgcgcaaggg
     7681 cgacatcacc tccgacttca gccccctgag gaacgaggaa accaacggct cctcccacac
     7741 cacgggaacc atcggcaatg gcgcgaccaa gttgcagatc aacgccgaca ccggagacat
     7801 tcgcatcaac aagggataga gtcgcactcc tgcagaacag caaaggccca gcccgttatg
     7861 cggattgggc ttttgtatgt cggtttcaag tctcaagttt cgagttggct aattgttcga
     7921 cgcgggagac cacatcatcg atcgcaaggt tggttgagtc gatgatcacc gcatccgcag
     7981 caggtacgag cggcgagacg gcgcgggtgc ggtcgcgttg gtcgcgggca accaggtctt
     8041 cgacgatccg ctgggcttcc tccgctgcga gcgtgccatt ctgagccacg cggcgctcgg
     8101 cccgaacccg ggcgtcggca tcgagaaaaa ctttcacatc ggcatcggga aacacggcag
     8161 tgccaatatc gcggccttcc atgaccactc cgccgctggc gcccatctcg cgctgacggg
     8221 aaaccatgat ctcgcgcacc ggcggatgca tggaaacgcg cgacgcggcc aaggtaacgt
     8281 cgctctgtcg tatgcgttgc gaaacatcgc ggccatcgag caacacgcgg ttgccttccg
     8341 cttgtggctc aagctcaatc acggtgccgg cggccaggcg gcgcaggact tccggatcgt
     8401 cgagcgacac ctgctgctcc agcgccttga gagcgagggc gcgatacatg gctccggttt
     8461 cgagattgat atagcccaac ttcttcgcaa ggcgggcggc gatggtgctt ttccccgagc
     8521 cggcagggcc gtcgatggca atcaccagtt tgcgggcagc gctcattcct tgggattatc
     8581 ctgacgggga ggtgcaggtc tcgacggtcg aatcttgcgg cgaggccgat gctccaccaa
     8641 cggctttcca gggcgcagtt cgggaggatt ctcttcggac cagggttcgg tacggcgttc
     8701 gcgcgccttg tgtggcaggc gggcttcggc ggcctgcagc atcacccgtc cgccaacgtg
     8761 tacgaagccg agctcgcgcc cagcctgcag ggcgttaatt gcatcgcgta ccttggcgcg
     8821 gcccaccagc ggagagaaaa agctttccac ctcgtcctgc ggcacggcct ggacacagtc
     8881 aatatatttc gaaactaatg cggtcagcga ctccggcacc gagaggtgca tgccttcttt
     8941 cacggcctcg ggagaccagc ggaagagcac gtcccagaag a
//
LOCUS       DASRKK010000131         5958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1279183, whole genome shotgun sequence.
ACCESSION   DASRKK010000131 DASRKK010000000
VERSION     DASRKK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279183"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFC15_03045"
     CDS             <1..270
                     /locus_tag="VFC15_03045"
                     /inference="COORDINATES: protein motif:HMM:NF021350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HZM09170.1"
                     /translation="KEMFRDAALKNIAILRLIENTLHAKRDRAIGEEFFRDILDEHFS
                     ADEVERQFETILNWGRYAEIFNYDSETGRLSHSDSADVTPTAGEA"
     gene            270..2027
                     /locus_tag="VFC15_03050"
     CDS             270..2027
                     /locus_tag="VFC15_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZM09171.1"
                     /translation="MARSTWLPTRIFGKGPWSLLPTQTWSVVTDFFIFAGGLSLFYVI
                     VVLARTWFGPFNPHVEISRSPAMLPVYAAYSLLRITVAYILSLAFTLVYGYIAAYNAR
                     AERIMVPLLDILQSIPVLSFLPGVMLAMVALFPHHQLGVELGAILLIFTGQVWNMAFS
                     FYSSLKSIPREMREAGKIYQFNWYQRFTNIELPYSAIGLVWNSMMSVAGGWFFLMACE
                     MFVLGNKDFRLPGLGSYLQTAAGAGDTHAILWGVGTMIAVIVLMDQIVWRPVIAWAEK
                     FKMEQVESADAPQSWFLDLIQHSRGLVGLRRRTVRPAQEWLALRFARQHHSAEPDAPP
                     PVWKVWVGRLIAAIAICAILYAMARATGMMSQLTREEYRELLTGTFATFLRVLAALFI
                     GALWTIPVGVAIGFNPRLARIAQPLAQIAASVPATALFPIVLLLLIKIGGGMGVGSIL
                     LLLLGTQWYILFNVIAGAMAIPTDLKEAATLFRFTTAQRWRRLILPGIFPYLVTGMVT
                     ASGGAWNASIVAEYFRLKDQTYTTVGLGAVISRATDAGNFDLLLASTIVMAIVVVTIN
                     RLVWRRMYRLAAARYTLEV"
     gene            complement(2078..2866)
                     /locus_tag="VFC15_03055"
     CDS             complement(2078..2866)
                     /locus_tag="VFC15_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09172.1"
                     /translation="MLKSMFPSQIALGLAQAAIAAIMALAVVLLARRKNVHLESDAAI
                     ALLRGIVQIVAVGSILALLLKAPRWTSVFLLVGMVLAAGSISAKRAKNIPGAAKVSTY
                     SIAAGAGLVTAIMTWAGVIDTAITSLIPVGSMIIANAMNTNGLALNRFRSEVLSHAGE
                     IETALALGAAPEATVARYAESSIHSSLIPAIDNLRSLGIVWIPGLMTGMLLSGANPMY
                     AAIYQFVVIAMILASSGLTSLLSTMMIRSHAFSPADQLVLRGAE"
     gene            complement(2859..3560)
                     /locus_tag="VFC15_03060"
     CDS             complement(2859..3560)
                     /locus_tag="VFC15_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM09173.1"
                     /translation="MSAPNHKAQLEAVLRAEKLGREVAGKTILSDISFDVATRDLLGI
                     VGASGSGKSSLLRLLNRLDEPTSGTVYLDGRDYRQLPPRDLRRRVGMVTQRPFLFPGD
                     AATNLRFGPAQRGETFPDAEIARLLERVGLSGFAASEVSVLSGGEQQRVSLARALANR
                     PEVLLLDEPTSALDEESKLGIEELVGSLVQDHDLTCVMVTHDRDQARRICNRVILLEA
                     GHLIRSGTAVEVLGA"
     gene            complement(3557..4333)
                     /locus_tag="VFC15_03065"
     CDS             complement(3557..4333)
                     /locus_tag="VFC15_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09174.1"
                     /translation="MSESVRDSVARFSDRANDYVKYRPHYSPEVVHALQQACGLRPGH
                     VVADVGCGPGLLAEILLKNGNRVIGVEPNLEMRAAGEQYLARYANFSTINGSAENTTL
                     ADGSMDFVTAGQAFHWFRPKEARTEFVRILKPHGWTVLVWHDRDTQATPFLRAYDNFL
                     QTYSIDYQQVNHKTVASLEVITQFFAPNSVQTISQQTRQLFDFDGLRGRLLSSSYVPK
                     EGAAAETMLQQLPDLFSKYAKDGRVTVEYETKIYYGHLIS"
     gene            5249..5587
                     /locus_tag="VFC15_03070"
     CDS             5249..5587
                     /locus_tag="VFC15_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM09175.1"
                     /translation="MDIERVEDSGGARSILRLHGPLLLGNFFSFQSMVRGDQSKVLIV
                     DVADVPYIDSAGIGCLVGAHVSRENAGRKLIVVGASERLLNTLKATKVDQLFTFARTV
                     EDARAKNPSS"
     gene            complement(5694..>5958)
                     /locus_tag="VFC15_03075"
     CDS             complement(5694..>5958)
                     /locus_tag="VFC15_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09176.1"
                     /translation="INFDIGLARNAAWFIAQRLANEPSILHEVTIDGLDLAVSVGGRA
                     ILYPPFKAEEVAEILAAEIKDARSVIETLARQQPDVVPTPTAN"
BASE COUNT         1165 a         1794 c         1662 g         1337 t
ORIGIN      
        1 aaggaaatgt tccgcgatgc ggccctgaag aacattgcca tccttcgcct catcgagaac
       61 acgctgcatg ccaagcgcga ccgcgcgatt ggcgaggaat tcttccgcga tatcctcgac
      121 gagcacttca gtgcggacga agtcgagcgg cagtttgaga cgatcctgaa ctggggccgg
      181 tacgccgaga tctttaacta cgactccgag acgggacggc tctcccatag cgactctgcc
      241 gatgtgactc ccaccgcggg tgaggcctga tggcgcgttc cacatggctc cctacgcgta
      301 ttttcggcaa aggcccctgg tcattgctgc ccactcaaac ctggtcggtc gtcactgatt
      361 tctttatttt tgccggcgga ctgtcactct tctacgtcat cgtggttctg gcgcgaacct
      421 ggtttgggcc atttaaccct cacgtcgaga tctcgcggag cccggcgatg ctcccggtct
      481 acgccgcgta ttctttgctg cgcatcacag tggcctacat tctttccctc gccttcaccc
      541 tggtttacgg atacatcgct gcctataacg ccagggctga gcgaatcatg gtcccgttac
      601 tcgatattct ccagtccatt ccagtgctta gctttcttcc aggtgtgatg ctggccatgg
      661 ttgctttgtt tcctcaccat cagttaggcg tcgagttggg cgccatattg ctcatcttta
      721 ccgggcaggt gtggaacatg gcgttcagct tctattcctc gctgaagagc attccgcgcg
      781 aaatgcgcga ggctggcaag atataccagt tcaattggta ccagaggttc accaacatcg
      841 aattgccata ctccgccatc gggttggtgt ggaactccat gatgtcggtt gcaggcggtt
      901 ggttcttcct gatggcctgc gaaatgttcg tgctgggcaa taaggacttt cgtctgcccg
      961 gcctgggctc atacctgcag acggcggccg gcgctggtga tacccacgcc attttgtggg
     1021 gcgtgggtac catgatcgcg gtcatagtcc tgatggatca gatcgtctgg cgtcccgtga
     1081 tcgcatgggc ggagaaattc aaaatggagc aggtggagag cgccgatgct ccccaatcat
     1141 ggttcctcga tctcattcag cattcgcgcg ggctggtggg actgcgcagg cgtacggtcc
     1201 gtcccgcgca ggaatggctg gccttgcgct tcgcccgcca acatcactcg gctgaacccg
     1261 atgcgccgcc gcctgtgtgg aaggtttggg tgggtcgact cattgccgcc atcgccattt
     1321 gcgcaatact ttatgccatg gcgcgcgcga ctggcatgat gagccagttg actcgcgaag
     1381 agtacaggga actcctgacc gggacctttg cgactttcct gcgagtgctg gccgccctgt
     1441 tcatcggtgc actctggacc atcccggtcg gcgtcgccat cggcttcaat ccccgcctcg
     1501 cccgcattgc gcaaccgttg gcccaaatcg cagcttccgt tcctgccact gccctgtttc
     1561 caatcgttct gctgctactg atcaaaatcg gcggaggaat gggggtcggt tccatcctac
     1621 tgctcttgct cgggacgcaa tggtacatcc tgttcaacgt gattgccggt gcaatggcga
     1681 taccaacaga tttgaaagag gccgcaactc tttttcgatt taccactgcc cagcgttggc
     1741 gcaggcttat cctgccggga atatttcctt atctggtcac tggaatggtg acagcttcgg
     1801 gaggagcgtg gaatgccagt atcgtagcgg agtattttcg cctcaaggac caaacctata
     1861 ccacggttgg cttgggcgca gtcatttctc gcgccaccga cgctgggaat ttcgatttgc
     1921 tgctggcttc taccatagtc atggcaatcg tcgtggtcac catcaatcgt cttgtttgga
     1981 gacgtatgta ccgcttggcc gctgctcgct acaccctcga ggtctgatgt tcgacccatt
     2041 ccgggtgaat ttgcgcgatt ctatgaactg accgtcgcta ttcagcgcct ctcaatacga
     2101 gttggtctgc gggtgagaag gcatgcgaac ggatcatcat tgtgctcagc agggaagtta
     2161 gccccgacga tgccaggatc atcgcaatca ccacgaactg gtagatcgcc gcatacatgg
     2221 gattcgcgcc ggagagcagc attccggtca tcagccccgg aatccacaca atccccagcg
     2281 aacgcaggtt atcgatggca gggatgagcg atgagtgaat gctcgattcc gcgtagcgcg
     2341 ccaccgtcgc ctcgggtgcc gcccccagcg ccagggcggt ctcaatctcc cccgcgtgcg
     2401 acagcacctc cgagcgaaaa cggttcagcg cgagcccgtt cgtgttcatt gcattcgcga
     2461 tgatcatgct tcccaccgga atcagtgaag tgatcgcggt atcaatcact ccggcccacg
     2521 tcatgattgc ggtaaccagt cccgcaccag ctgcgatgga atacgtcgaa accttggcgg
     2581 cgccgggaat gttcttggcc ctctttgccg agattgaccc tgctgccagc accatgccca
     2641 ccagcagaaa tacgctggtc caccgcggcg ctttcagcag cagagccagg atcgaaccaa
     2701 ccgcaacgat ttgcacgatg ccgcgtagca aggcgatggc cgcgtcgctt tccaagtgaa
     2761 cattctttcg ccgagccagc agcacaaccg ccagcgccat gatcgctgcg atggcggcct
     2821 gtgccaagcc caacgcaatc tgagacggaa acattgactt aagcacctag cacctccact
     2881 gcagtcccgc ttcgtatcag gtgtcccgct tcgagcagga tcacgcgatt acagatccgg
     2941 cgcgcctggt cgcgatcgtg cgtcaccatc acgcacgtca ggtcgtgatc ttggaccagg
     3001 ctcccgacga gttcttcgat gccgagcttc gactcctcgt ccagcgcgga tgttggctcg
     3061 tcgagcaaca gtacctcggg ccgattggcc agggcgcgtg ccagcgacac cctctgctgc
     3121 tcgccaccgg agagaaccga cacctcgctc gcagcaaacc cggacaaccc cacgcgctcg
     3181 agcaggcgcg caatctccgc gtctggaaag gtctcacccc gttgtgctgg cccaaaacgc
     3241 aagttcgtcg ccgcgtcgcc cggaaaaaga aacgggcgct gcgtgaccat gccgacacgc
     3301 cgccggaggt cacgcggagg cagttgacga taatcccgcc catcgaggta aacggtgccg
     3361 ctggtgggct cgtccagacg attcagcaag cgcaacaacg aacttttgcc ggagccgctg
     3421 gctcccacta tgcccaacag atcacgggtt gcgacatcaa acgatatgtc gctgagaatg
     3481 gtcttgcccg caacctctcg acccagcttc tcagcgcgca gcacggcttc gagttgagcc
     3541 ttgtggtttg gggcactcat gaaatcaagt gcccgtagta gatcttggtc tcgtattcga
     3601 cagtcactcg gccatctttg gcgtatttcg agaagaggtc gggcagttgt tgcagcatcg
     3661 tttccgccgc tgcgccctct ttcggaacat acgacgacga caggagccgt cctcgcaagc
     3721 cgtcgaaatc gaaaagctgc cgggtctgct gcgagatggt ttgcaccgag ttcggagcaa
     3781 aaaactgcgt gatgacctca aggcttgcta ctgttttgtg gttcacctgc tggtaatcga
     3841 tggaataggt ctgcaaaaag ttgtcgtacg ccctgaggaa gggcgttgcc tgcgtatccc
     3901 tgtcatgcca gacaagaacc gtccaaccat gcggcttgag aatgcggaca aattcggttc
     3961 gagcctcctt tggtcgaaac caatggaatg cctgtcccgc cgtgacgaag tccatagatc
     4021 cgtcagcgag cgttgtattc tccgcgctgc cgttaatcgt gctgaagttg gcgtaacgtg
     4081 ccaggtactg ctcaccggcc gcacgcattt cgagattcgg ctcgacgccg atcacccgat
     4141 ttccgttctt caacaggatc tccgccagca atccgggacc acaaccgaca tccgctacga
     4201 cgtgtccggg ccttagaccg catgcctgct ggagcgcgtg caccacctcg ggagaatagt
     4261 ggggacgata tttcacgtag tcattcgcgc ggtcagaaaa ccgcgctaca gaatcgcgta
     4321 ccgactcgga catgggtgtt atcttatcgt gagcgatgaa gcgcccgcct acttccaggc
     4381 ctccaattgc tgccgccgtc gccgcggtga tccgtcagat tccccgtgga cgggtcgcca
     4441 cttacggtgc cgttgccgag gcggctggtt acccacgctg tgcccgtcat gtttcgaagg
     4501 tcctgagcca agtgcgagga ttgccttggt atcgcgtgct gggcagcgga ggccatatct
     4561 cgttgcgggg cgaatatggg tttgagcagc gatttctgct ggaatccgaa ggggtgagat
     4621 tcaaggggcg ccgtgtcgat atggtgacct gcgaacatca cttcccgggc actcgccgca
     4681 gggcctctac cgggaagagg tgaggcctgc agtccctcga aagtggcatg acgagtatcc
     4741 aagaagcctc tttccaattg ttgtcagatt ggcataaaat taattggggt tgtcgatcgc
     4801 tcaccacttt atgccgcaat ttcgctccac tattcgccga atagcttttt cgttgctgct
     4861 cttgtccaca ggcctttctg ctcagcgcta cactggccat gatcatgcgg tatttcatcg
     4921 ccagccgcag agccctccgc ccgcggcaaa gcaccaatcg ttgtccggca acacagccac
     4981 aagtcacccc aagaccgcgg cgtcgccatc cgcgtcgaat cagcagggcc cacgcgctgg
     5041 cgctgcaacc ggcaagctag tggacgactt gaaccacaac tctgctcctg ccaagaccga
     5101 tccgcctctc gaaaacgccc ctcatttgta gctaacgtcg tcagcgcaat tggctcgact
     5161 gaacgaccga ttccgtcgcg gtaaacaaaa attaacactc ccgtagcact gtcgggttat
     5221 aagatacata tctatgcagg acaagccctt ggatatcgaa cgcgtcgaag attctggcgg
     5281 agccaggagc atattacgtc tccatggtcc actgttgtta ggaaactttt tcagcttcca
     5341 gtcgatggtg cggggtgacc agtccaaagt gctcatcgtg gacgtggctg atgttcccta
     5401 catcgattcg gctggcatcg gatgcctggt gggtgcgcac gtatcgcgag agaacgccgg
     5461 ccgtaagctc attgtggtcg gtgcctccga gcgtctgctc aacacgctga aagccaccaa
     5521 ggtcgaccag ttgtttactt tcgcgcgtac agtcgaagac gcccgcgcta agaatccaag
     5581 ctcatagtct cgttgggtag tctaagcaga ccgcatcgga ttgggattct cgcgcgccag
     5641 tggcgggagt gccttccatc attccgtccg ctcagatcgc cgtgcgtggt gtttcaattg
     5701 gctgtcggcg ttggtaccac gtccggttgc tggcgcgcca gtgtctcgat gacgcttcgt
     5761 gcgtccttga tttctgcggc gaggatctcc gcgacctctt cggctttgaa tggcggatac
     5821 aaaatcgccc gtccccccac agatactgcc aagtcgagtc cgtctatcgt gacttcgtgc
     5881 agaattgacg gttcgttggc cagtcgctgc gcaatgaacc aggctgcatt ccgcgctagt
     5941 ccgatgtcga agttgatc
//
LOCUS       DASRKK010000132         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1083746, whole genome shotgun sequence.
ACCESSION   DASRKK010000132 DASRKK010000000
VERSION     DASRKK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083746"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFC15_03080"
     CDS             complement(<1..735)
                     /locus_tag="VFC15_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09177.1"
                     /translation="MVALIISVLGHAQQEPRTNPNDLVRRAAANEIKANDPHVYFMFK
                     DRTEEKGHSITKEVIQTPQGGLTRTLAINDKPPTAEQRAKDDQKLQKFANDPEARRKR
                     KAANKEEDERDALMLTSLPDAFLYTYAGEEPGPNGASLVHLTFKPNPKFDPPNHETMV
                     YQGMQGDMLIDPKAARIAKIDGTLFKDVDFGWGILGRLYKEGKFLIEQRDVGGGHWEA
                     VRQVLQFNGRILLVKSLTISSAETTYD"
     gene            1167..1832
                     /locus_tag="VFC15_03085"
     CDS             1167..1832
                     /locus_tag="VFC15_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM09178.1"
                     /translation="MRRIVIPELLDDDLGTAAEIAESLTDLRHINQWFGGMRTTTELL
                     RRIAREVGHRKLSILEVGAGSGYVPLAAQRVLGGWGIGLQVTLLDRVWSHLPQGRAAS
                     VAGDALRLPFRDGAFDVVSCSLLAHHFEPETLVEFACESLRVSRQAVLINDLIRSRLH
                     LLLVYLGLPLFRSRITWHDAPASVRRAYTCDEMRGVLANVGARKVQVSRHYMFRMGVL
                     LWK"
     gene            1920..>2108
                     /locus_tag="VFC15_03090"
     CDS             1920..>2108
                     /locus_tag="VFC15_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM09179.1"
                     /translation="MPNAKRQMLNPDPQFDLAIVGAGPAGSSSAITAARLGARVGLFE
                     AKDFPRHRVCGEFVSAESL"
BASE COUNT          402 a          589 c          607 g          510 t
ORIGIN      
        1 atcgtaggtc gtttccgcgg acgagatcgt cagcgactta accagcagga tcctgccatt
       61 gaactgaagg acttgacgca ccgcctccca gtggcctccg cccacgtccc tttgctcgat
      121 caagaacttg ccctctttgt agagcctgcc caaaatgccc cagccgaagt ccacatcctt
      181 gaacagcgtg ccatcaatct tggcgatgcg cgccgctttc ggatcgatca acatgtcgcc
      241 ttgcatgccc tggtacacca tggtttcgtg gtttggcgga tcgaatttcg ggttgggctt
      301 gaaggtgagg tgcacgagtg acgcgccatt agggcctggc tcctctcccg cgtaggtgta
      361 aagaaacgcg tcgggcaggc tggtcaacat aagcgcgtcg cgctcgtctt cctccttgtt
      421 cgctgccttc ctcttacgac gcgcctcggg atcgttggcg aacttctgta gcttctgatc
      481 gtccttggcc cgctgttcgg ccgtcggtgg cttgtcgtta atggctagcg ttcttgtcag
      541 gcccccttgg ggagtctgga ttacttcttt ggtgatcgaa tgaccctttt cctcggtgcg
      601 atccttgaac atgaaataaa catggggatc gttggctttg atctcgttgg ctgcggcgcg
      661 gcgcaccaga tcgttggggt tcgtccgcgg ctcctgctgg gcgtgaccca gcacactgat
      721 gattaaggcg accattacta ccgatacggc tcgacccgta tgctttcctg acaaatccac
      781 atttacctct aagcttgacc gcaaaattac tcccaagcaa ttagacgaaa tgctgagcgt
      841 cttagttgct ttttcgacaa gcccgttttt gttgccggga aaaggtctgc aactcggatg
      901 cagtcgccct gaagtacttc ggcagggttg cggcatcctc cagttgaatg tcgcccacga
      961 tggagttccc caattcctat cttcccgttc gattggctaa tctcaaggtc agcatcgact
     1021 ctgggaccga tcgattttcg gagaatcggt aaaatctatc cagcgggtat agatttgtga
     1081 ggcggcctcg taatgccgaa ggtgcgatgt caaccttgga cgaacgatct cggatcgggc
     1141 ggccgatggc accgcttcca ctctgaatgc gacgaattgt cattcccgag ttgctcgatg
     1201 acgatctcgg cacagccgcg gagattgcgg aaagtctaac cgatttgcgg catatcaatc
     1261 agtggttcgg cggaatgcgc acaacgaccg agctactccg ccgaatcgcg cgcgaagtcg
     1321 gccaccggaa actctcgata cttgaagttg gagcggggtc cggatacgta ccgttggcag
     1381 cgcaacgtgt gctcggaggg tggggcattg ggttgcaggt gacccttctc gatcgcgtgt
     1441 ggtcgcatct gccacaggga cgcgcagcct cggttgcagg cgacgccttg cgcctgccct
     1501 ttcgggatgg cgcattcgac gtcgtaagtt gttcgctgct ggcccatcat tttgagcctg
     1561 agacgctggt ggaatttgcc tgtgaatccc tacgcgtctc ccggcaggcg gtgctgatca
     1621 atgatctcat tcgcagccgg ttgcacctgt tgctggttta ccttggattg ccgctcttcc
     1681 gcagccgcat cacgtggcat gacgcgcccg catcggtccg gcgggcttac acctgcgacg
     1741 agatgcgcgg tgtgctagcg aatgtaggcg ccagaaaagt tcaggtttcg cgacattaca
     1801 tgttccgaat gggtgtgttg ctgtggaagt gagaaaagca gcagttagcg gttagcgctt
     1861 agcacttagc aactacagag attcgcacaa tataagcttc gtcatcgcga gcctgccaaa
     1921 tgccaaatgc taagcgccaa atgctaaatc ctgatcccca attcgacctc gcgatcgtgg
     1981 gagcgggccc ggcgggaagc tcctctgcga ttaccgcggc gcgcctgggt gcaagagtcg
     2041 ggttgttcga ggcgaaagac ttcccgcgac atagagtctg cggagaattt gtttccgcgg
     2101 agtcgttg
//
LOCUS       DASRKK010000133         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1279325, whole genome shotgun sequence.
ACCESSION   DASRKK010000133 DASRKK010000000
VERSION     DASRKK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279325"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1283
                     /locus_tag="VFC15_03095"
     CDS             630..1283
                     /locus_tag="VFC15_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM09180.1"
                     /translation="MPSKRTLTFSPGMFLKRIGSRRSSREYRNKQPIFSQGDAADAMF
                     YVEAGNVKLTVLSKRGKKAVIAIFRQGDFFGEGCLGAPSLRMSTATAVHPATIARVEK
                     PIIVRIIHEDPVFARLFIAHLLSRVARIQDDFLDQLFNSSEKRLARTLLLLAGLGTQS
                     KPYSADLKVSQGTLAEMVGTTRSRVSFFMNRFRKMGLIDYNGSVRVHKELLTFLLHE"
     gene            complement(1367..2740)
                     /gene="mtaB"
                     /locus_tag="VFC15_03100"
     CDS             complement(1367..2740)
                     /gene="mtaB"
                     /locus_tag="VFC15_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZM09181.1"
                     /translation="MATYHVENFGCRATQADGAAIERQLLGRGLERASAAVEAEVVIL
                     NTCTVTASADQDVRAAIRRIHRENPAARILVTGCYAQRAPEELSALPGVSLVLGNSHK
                     HELANYAVPLRSDTGFVALDQIGLNPASIVIVGDIFAHTELMAAPVFAAGSFEKTRPN
                     LKVQDGCNNRCSFCIIPSVRGRSRSLKLEQILREVDGLVANGYREIVLSGINLGRWGR
                     ELTPRMNFAAMLRAILERTAIEKVRISSVEPMDWTNEVIELVASSPRIAKHAHVPLQS
                     GSDRILRKMHRKYRPWHYADRIERIRRAMPDAAIGADVMVGFPGETDDDFEQTRALIQ
                     RLPFTYLHVFTYSSRPGTPSAEMPEQVPVHIARERNRVLRELAVQKKREFMQSFVGRE
                     LQAITLTHYDGECTEALTDNYLKLKLRGKHEANRWVRSLIEAVHSDALMGLLAVPASA
                     IPAGAPA"
     gene            2929..3393
                     /locus_tag="VFC15_03105"
     CDS             2929..3393
                     /locus_tag="VFC15_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM09182.1"
                     /translation="MKRRILLVDDELAILLTLRAILEMNDFEVDTASSAKEAEGKLKN
                     GVFEMVITDMRMKTETAGYDVIRAARQLAYNPATAILTAYPSLGNEWQSHGAQSLLVK
                     PVNTAELLRQIEALLISHQDHKMQAASANARKVPNPNNSKKRRPATAGKKAM"
BASE COUNT          740 a          964 c         1015 g          727 t
ORIGIN      
        1 agttggcccc tttgcacgta aaaaatcgtg tcggcgggat cgccttgcgc gtaggctacc
       61 tcattcttct taagactaac aacttttctg cccaacccag ttctggtgag aaagggctca
      121 gcatcgaaga ccgtattgcc attttttggt tgcggcttgg gcgttggaag ctgggaaggc
      181 tttgcaattt cgcctgtggg ggatttggcg gatcgtacag gcccgaccga tggaccccgt
      241 ttggctagtg ggccttggct gcgggttgga atgcccctgg tgctcatttc atacctcctc
      301 gggggcagcg gttacgcttc cctattatac cccctcttca agaggcggag atatgcactt
      361 actgttcaat attgtacagg ttaccagttg ttaatttcgg ccgtttgagc gattttgcac
      421 agccctctca gttcctaagc ggtaggctca acgcgtgagg gaattttgaa ggcgcgggga
      481 catgcgagac cctgaacgga cggagtatcc ctcgcgaagt tcgtcgatat tgggcgttgt
      541 ttacgccttg atcgagattg atgtaccgcg aaacggacgc ttcgacccgt gcaacagaga
      601 aggaagatca acccttatcg gagacgttaa tgccaagcaa acgcacgctt acttttagtc
      661 ccgggatgtt tctcaagagg atcggcagcc ggaggagcag tcgggagtac cggaacaaac
      721 agccgatttt ttcccagggc gatgctgccg atgcgatgtt ctatgtcgag gctggcaacg
      781 tgaagctgac ggttctatcc aaacgtggca agaaggccgt catcgctatt tttcggcaag
      841 gcgacttttt cggtgaaggc tgtttaggag cgccgtcttt gcggatgtcc accgcgaccg
      901 cggtacaccc ggccacaatt gcgcgagtgg aaaagccgat catcgttcgc atcatccacg
      961 aagatcctgt gtttgcaagg ctatttatcg cgcacctgct ttctcgcgtc gcccgaattc
     1021 aagacgactt cctcgatcaa ctcttcaatt ccagcgagaa gcggctggcg cgaactcttc
     1081 tgttgctcgc gggcttgggg acgcaatcca agccatattc cgccgacctg aaggtgagcc
     1141 agggaaccct ggccgagatg gttggcacca cccggtcgag agtaagcttc tttatgaacc
     1201 ggtttcgcaa gatgggcctc atcgattaca acggcagcgt gcgggtgcac aaggaactgc
     1261 tcaccttcct tctccacgaa taggcgccac acctcgcgtg cgctccgctc aaggaaaaag
     1321 cgaaagtgtt taaattccga ccagttttga gacgagcaag agctcgttag gcgggagcac
     1381 cggccggaat tgccgacgcc gggactgcaa gcaagcccat cagcgcatcg gaatgcaccg
     1441 cttcgatcag gctccgcacc caccgattcg cttcgtgctt gccgcgcagc ttgagcttca
     1501 gatagttgtc ggtaagcgcc tcggtacatt cgccgtcgta atgcgtgagt gtgatcgctt
     1561 gtagttcgcg gccgacaaac gactgcatga actcgcgctt cttctgcacc gccagctcgc
     1621 gcagcacgcg attgcgttcg cgcgcaatgt gtaccggaac ctgctccggc atttcggcgg
     1681 acggagttcc cgggcgcgaa gaatacgtaa aaacgtgcag ataggtgaac ggcaggcgct
     1741 ggatcagagc gcgcgtctgc tcgaagtcgt cgtcggtctc accgggaaag ccaaccatta
     1801 cgtcggcgcc aattgcggca tcgggcatgg cccggcggat tcgctcgatg cgatcggcat
     1861 aatgccaggg gcggtacttg cggtgcatct tgcgcagaat gcgatcgctg cccgattgca
     1921 acggcacgtg cgcgtgcttg gcgatgcgcg gtgacgacgc gaccaactcg atcacttcgt
     1981 tcgtccagtc cataggctcg acggagctga tgcggacctt ctcgatcgcc gtccgctcga
     2041 ggatggcgcg cagcatggcg gcgaaattca ttctcggcgt aagttcgcgt ccccagcgcc
     2101 ccaggttgat gcccgacagc acaatctcgc gatagccgtt ggcgaccaac ccatcgactt
     2161 cgcgaagtat ctgctccaac ttcaacgacc ggctgcggcc gcgcaccgac gggatgatgc
     2221 agaacgagca gcgattattg cagccgtcct gcactttgag attgggacgt gtcttctcga
     2281 aggagccggc tgcaaagacg ggagcggcca tcaactcggt gtgagcgaag atgtcgccga
     2341 cgatcacgat tgacgcagga ttcaggccga tttgatccaa tgctacgaat ccggtgtcgc
     2401 tcctcagcgg aacagcgtaa tttgccaact catgcttgtg tgagtttccg aggaccaggc
     2461 tgacgccggg cagcgccgaa agctcttcag gcgcgcgctg agcgtagcag ccggtgacca
     2521 ggatcctggc ggcgggattt tcgcgatgaa tgcggcggat ggcagcacgc acgtcctgat
     2581 cggcagatgc cgtgacggtg catgtgttca ggatgacgac ctcggcctct acggcggcgg
     2641 aggcacgctc tagaccccgg cccagcaatt ggcgttcgat ggcggcgcca tcggcctggg
     2701 tcgcgcgaca tccaaaattc tcaacatgat aagtcgccac caagctatta tactggcgct
     2761 tacttcttaa gcatgttgag catggcagcg cgtccggcgt ggtggaagat cgaaataatt
     2821 agtgggacgc gcacggaatg agaatgcggt taggagcaac ttggcggcag gaacttgcat
     2881 tacacccgcc gcacgttgat gatgagactt tccgcgtgga gttccccgat gaaacgtcgc
     2941 attctgttag ttgacgatga acttgcaatt cttctcaccc tgcgcgcaat cctcgaaatg
     3001 aacgattttg aagtggacac cgcctcctcc gccaaggaag ctgagggaaa actaaagaac
     3061 ggcgtcttcg agatggtgat tactgatatg cgcatgaaaa cggaaactgc gggctacgac
     3121 gtgattcggg cggcgcgtca attggcttac aacccagcca ccgcgatact gactgcatac
     3181 ccgtccctgg gcaacgaatg gcagagccat ggagcgcaat cgttgctggt gaagccggtc
     3241 aacacggcgg aactgctgcg ccagatcgaa gcgctcttga tcagccatca ggaccacaaa
     3301 atgcaggcag cgagtgcgaa tgccaggaaa gttcccaacc ccaataactc caagaaaagg
     3361 cgccccgcca cggcgggcaa gaaggccatg tagcattacc gggaagttcg gctgtccagc
     3421 gctgcatctc gtgatcggtc tggaac
//
LOCUS       DASRKK010000134         8160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_399847, whole genome shotgun sequence.
ACCESSION   DASRKK010000134 DASRKK010000000
VERSION     DASRKK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399847"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFC15_03110"
     CDS             <1..369
                     /locus_tag="VFC15_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09183.1"
                     /translation="GSTVYVTLYSLVGAQWVANSYTYTAFNTAGALGTMQTPIDGSTL
                     SGNVAAFTWSAGTGATAYWLDIGSVPGGNQYYQSGNLGNVLTTTVYSLPVDGSTIFAT
                     LYSYVGGQWLSNAYTYVSNP"
     gene            2889..6287
                     /locus_tag="VFC15_03115"
     CDS             2889..6287
                     /locus_tag="VFC15_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZM09184.1"
                     /translation="MGAGTIQTFTLSASLFGVADPLTPGLTPANVTFTQGNTDTGSVT
                     VVPSGTQPGNGTFSFDATGGVIGSVNLTANIDTFASNTVSLNVIAPALYSPVNGSTLP
                     GSAVTFYWTEYPGATAYWLDVGATPGGNTYAQSGSLSSSTLSYTVNTLPTDGSTVYAT
                     WYSLVNGSWRHSDYSYTAYTAGSSKGVITSPVPGSTLTGSTVNFIWTLGNGATNYWID
                     AGSTVGGNQYFQSGPLGNVNTKTVTGLPTDGSTVYVTLYSLVGGTWLNNQYTYTAYSV
                     VVGPPPVSFLTAVNYGAGSGPDSVAAGDFNGDGKADLAVANYGDGTVSVLLGNGDGTF
                     QSAVNYPVGSAAALVVVADLNGDGKLDLAVADANGVSVLLGNGDGTFQPAVNYPAGTN
                     PFWVAVADLNGDGKLDLAVADGGGGVSVLLGNGDGTFQAAVNYGAGASPYSVAVGDFN
                     GDGKPDLTVANYGDGTVSVLLNNGNGTFQTAVNYSVGSSPYAVAVGDFNGDGKLDLAT
                     ANYASGNVSVLIGNGNGTFQPAVNYAAGPGANSVSVADFNGDGKLDLAVADRNSNNVS
                     VLLGNGDGTFQPPANYGVGAGPAGSVAGDFNGDGKPDIATANYGANSVSILLSNTAFQ
                     GPAIVYSPAPNSTLPGPSVTFQWTPSDSATAYWIDVRSSAGDNQYYQSGSLLTTTLSA
                     TVNGLPLNGSAVYATMYSLIGGQWVHNSYTYTAFTGSSKGVITTPANGSTFTGTTVTF
                     GWTAGAGASAYWLDAGSTPFGNNYYQSGNLGNVLTTTVSGLPSDGSTVYVTLYSYVGG
                     QWLSNSYTYTAFTPIVTLGVMQTPIPGSTLSGNTATFTWSAGVGATAYWLEVGSSPGG
                     NQYYQSGNLGNVLTTTMYTLPQNGSTVYATLYSYIGGQWLSNSYTYGSAMAFQGFETD
                     IGDWQPITTRVASGGGVLGVSSASGSYHAELTNVYDTYGTGYGGAEYSLFGYATQPPY
                     PGDFSQSISMYIDKNWPTASPNGNGVGVWIDMSPGNPDPNNYGAEHNFRFTPSGTAVA
                     VSVDGQGSPIATITTSGWYKFQMTYQKGALPTDLVITNMNVFDSTGHLVGTTTVLANS
                     PGGPLLSENLLGPGYVWITVWADGWPAAGANDVLAIDDVRTDLL"
     gene            6468..6881
                     /locus_tag="VFC15_03120"
     CDS             6468..6881
                     /locus_tag="VFC15_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09185.1"
                     /translation="MDANNQSNNAGQFELRKEKDGSFVVVGVGAHGPNGESSSQQPIL
                     DSLISLTAERRSARQTIALICQKVSQQSKIQVTADGVADSLPEPKTVAVGGVGVPART
                     LLSRTLASMRGKLYWRLIYDSNEKSYQLSISRESQ"
     gene            complement(6985..7794)
                     /locus_tag="VFC15_03125"
     CDS             complement(6985..7794)
                     /locus_tag="VFC15_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZM09186.1"
                     /translation="MSLQLGVGDLLEMSVYGVPELATKTRISSNGDMYCPLIGYTHVA
                     GLTAEEAQKLIETRLADFVKNPHVSLFVTDYASEGASVLGAVARPGVYPVLGQQHLFD
                     LISAAGGLSEKAGRSLTVTHRSDPDKTATIDLSRNLKDNPDTNVPVFPGDTIIVRKAD
                     IVYVVGDVTRPSGLLMEAGGLTVLQAVALAGGTTRTAKLSGAKILRKGPGGMTETPVE
                     LKKILQAKAPDLQMQADDILVVPSSTGKVIAGATLQAALQAATLLSVAAVP"
BASE COUNT         1743 a         2396 c         2347 g         1674 t
ORIGIN      
        1 ggcagcacgg tgtacgtgac actgtactcg ctggtgggag cacaatgggt agccaactcc
       61 tacacctaca cggcgttcaa caccgccgga gccttgggga ccatgcagac gccgattgac
      121 ggatcgacgc tgagcggcaa cgtcgctgcc ttcacctgga gcgccggcac aggcgccaca
      181 gcctactggc tggacatcgg gagcgttccg ggtggcaacc agtactatca gtctgggaac
      241 ttgggcaatg tgctgactac cacggtgtat agtttgccgg tcgatggcag cacgatcttc
      301 gcaaccctgt actcctacgt tggaggccag tggttgagca acgcctacac ttacgtttca
      361 aatccgtaaa gggacgaaat gactcatcgc cgtcggtctc cagcggctgt ggatttcacc
      421 agggccgcgc caaaaacgca tccagcctgt gtttggctgg atgcgcggcg cgacccgagt
      481 cgagggacct gcggttcgcc ggtaccgtta cgccgtcggg aacaaccgca gatccttcga
      541 ctgcgcccga ccgacctaca aaagcagcgg gtcggaaatt ctttgcgggc gctccgttca
      601 ggatgacacc tcaacgttca tgaatagtcc agctgaaatg cacttccggg tggaagcccc
      661 cgcatttatg cgggggaagg agcgcttcag cgctccggga aaaattcgac tttgatcacg
      721 tgctttagcg ccgggctcga gaaatcccgg ggctgaagag ggtgtgtgcg aactgttccg
      781 tccctcaacc ccccaacaag cgcaaaagcg gcgcttgttg gggatcccgc taaggaactc
      841 ggatctactt cccactctac ccagcactcc gcctgcggct gcgtgctggg ctaaactgag
      901 ccgcgcctac ggcgctggat tttcggccaa gtcttttcgc accacataaa tgctattcca
      961 ttctgctaca accaaagtag tattgccttg ctatagccgt taagactatt gtccgtcttg
     1021 tcacccaggc agggttcaat tagccatttt tccaaatgga attgatgctt tagacagaat
     1081 gtttgactga gccgaagagg aattgactcg tctttcggtt tggttgttca caatctctca
     1141 gcagtctaaa tcccattgac ccacctgtgc gatgtgcccc ctaaagtttg gcagttgttc
     1201 gcattgactg gccgatcatt ggttgtgatt gcacgcgcag caataagagc catatattgc
     1261 gcgatcaagc ccgcgccact tcgtacccgc ctgggcataa gcgcctgaag ttcatgttgt
     1321 cctgaagcac gaaggtcatc ggacccatga gaagaggcag gtttttctga acagccttgg
     1381 ttctgagcct atggcgcaag gaattgcgta caggggttta ctgtagtcgg attcggtgcc
     1441 ctggatcatc tccacacgag atcaagtcct agtcgccttt aacaaccgga tcacgctgag
     1501 gatcgggaag cgaagcctcc ccacgcctct gcagctttac gagagaggat caataccatg
     1561 ttcacccgtt ctaccagcct ttcaatcaca gcggtcctgg ttgtcgcatg cgtactcgcg
     1621 ttcgcacagg gcggcccggg gccacctctc cgtaaactta caacttagaa acgcctgcct
     1681 ccggggtgcg gatagggccc cgatacagtg cccaagaacc aaatcatgca gggtagcagc
     1741 ggatcgtctc aacatccgag gagggtcatc atgtcattaa gaaaatttgc cgtttcttcc
     1801 agaaggccgg tgttcggcct cgctctcatt ctcattctgg cagtgggagt tacttgcaag
     1861 tgggctagga atcgtcttcc agggattcga aactgacgta agcatgtggt tcaacgaggg
     1921 cagcgtccag caaaccgttc aagagccttc ggggtacacc aacggtgggt atgccagcgg
     1981 cattccatcg gcgacaggga acttccacgc tcggctcgtg atcgacccgg gtcaatgcga
     2041 cccactccat cccaataatg actgcgctgg gccctacaca tattggaacg aaatccgtac
     2101 aagcgacggg acgaccactg gcgggtcaaa catagtcacg tcagccacag cgaacttcaa
     2161 agccgccgat gttgggcgtt ccgtgtacgt caataaccct gcgaccggcc acttagtctt
     2221 tccagccgga accacggtca gcagcgtgag caactcaacg acggctctgg caagtgcggc
     2281 cgcggtagag agctctaccg gcgcgcccct gagggtcttt tcgctatttt cgctcggcta
     2341 tatcacccag gccgatatct atctcgatac ggcatgggcc gccggaaatc ccgatcggcg
     2401 tttcgattgg gacacagcaa tacgagataa cgctctaaac ttcgactcgg actacgcgtt
     2461 caacgtagga acgcagctct ctggcgatag taccgcgggc ttttggataa gtacgagtac
     2521 caataccaat cgcggcaata ccaacccgga gaatccatgt cccagccctg ctaacgacgg
     2581 tatcggaaac gtttgccggg cgcctttcaa gatcacgacc agcggctggt acactttccg
     2641 gcacatcttc cggatcgatc ccaataccca aaatctttcc gtcgaactcc aggtcatgcc
     2701 tcaaggtgga agtccggtgg tggacaaaac gatctacggg tggcagagtc acgcgcaagc
     2761 gccagcaggc ggtattatgt cggcctatgg cttgttcgcg aaccaggaaa tcccgcagct
     2821 ggccatcgac aatgcgctac tgaaggattt cgacgcgctc gcgctatctc ccaccaaatc
     2881 ccagcccatt gggggcgggg acgattcaaa ccttcacgct ctcggccagc ttgttcggag
     2941 tagccgaccc acttactccc ggactcactc cggcgaacgt cacgttcacg cagggcaaca
     3001 ccgatacggg ctcggtcacc gtcgttccca gcgggacgca gccgggcaat gggacgttct
     3061 cgttcgatgc gacgggcgga gtcattggtt cggtgaatct caccgcaaac atcgacacgt
     3121 ttgcgtcgaa cacggtctcg ctcaacgtga ttgcgccggc gctctatagc ccggttaacg
     3181 gatcgacgct gcccggcagt gcggttacct tctattggac cgaatatccc ggcgcaaccg
     3241 cgtattggtt ggatgtggga gcgacaccgg gtggcaacac ttatgcgcag tcgggcagcc
     3301 tcagcagttc gacgctgtct tacacagtca acactctgcc tacggacggc agcacggtat
     3361 atgcgacctg gtactccctg gtcaacggct catggcggca cagcgactac agctatacgg
     3421 cgtataccgc gggctcgtcc aaaggcgtga taacctcgcc ggttccgggc tcgaccttga
     3481 ccggaagcac ggtcaacttc atctggacac tgggcaacgg cgctactaac tactggatcg
     3541 acgccggcag cactgtgggc ggcaaccagt acttccagtc tggacccttg ggcaatgtga
     3601 acaccaagac cgtgaccggt ttaccgacgg acggcagcac agtctatgtg accctctact
     3661 cgctagtggg gggtacttgg ctcaacaacc agtacaccta cacggcctac agtgtggtgg
     3721 tgggtcctcc tccggtatcg ttcctgaccg cggtgaacta cggcgcgggc tctggtcccg
     3781 attccgtggc ggcgggggac ttcaacggcg acggcaaggc cgacttggcg gtggccaact
     3841 acggtgacgg aaccgtgagc gtgcttctag gcaacggcga cggcaccttc caatcggcgg
     3901 tgaactatcc cgtgggttca gcggccgcct tggttgtggt tgccgacctc aacggtgacg
     3961 gcaaactcga cctggcagtg gcagacgcga atggcgtgag cgtgcttctg ggcaacggcg
     4021 acggcacctt ccagccagca gtgaactacc cagccggcac caatcccttc tgggtggcgg
     4081 tagccgacct caacggcgat ggcaagctcg acttggcggt ggccgacggc ggtggcggcg
     4141 tgagcgtgct gctgggcaat ggcgacggca ccttccaggc ggcggtgaac tacggcgctg
     4201 gtgcatctcc ctactcagtg gcggtggggg acttcaacgg cgacggcaag cccgacttga
     4261 cggtggccaa ctacggtgac gggaccgtga gtgtgctcct gaacaacggt aacggcacct
     4321 tccagacggc ggtgaactac agcgtgggtt caagtcccta cgcggtagcc gtaggggatt
     4381 tcaacggcga cggcaagctt gacctggcga cggcaaacta cgccagcggt aacgtcagcg
     4441 tgctgatcgg caatggcaac ggcaccttcc agccggcggt gaactacgcc gcgggcccag
     4501 gcgccaactc ggtatcggta gcagacttca acggcgacgg caagctcgat ctggccgtgg
     4561 cagaccgtaa tagcaacaac gtgagcgtgc tgctgggcaa tggcgacggc accttccaac
     4621 cgccggccaa ctacggcgtc ggggcagggc ccgccggttc ggtcgcgggg gacttcaacg
     4681 gtgacggcaa gcctgacatc gcaacggcaa actacggtgc caacagtgtc tcgatattgc
     4741 tgagtaacac ggcattccag ggaccggcaa tcgtgtacag cccggctccc aactcgaccc
     4801 tgcccggacc ttcggtgacc ttccagtgga cacccagtga ttcggccacg gcctactgga
     4861 tcgatgtgcg ttccagcgca ggcgacaacc agtactacca gtcggggagc ctgcttacga
     4921 ctaccctctc ggccacggta aacggcttac cgctcaacgg cagcgccgtt tacgcgacca
     4981 tgtactcgct gatcggtggt cagtgggtac acaactccta cacctacact gccttcaccg
     5041 gttccagcaa aggcgtgatt acaacaccag cgaatggctc aaccttcact ggcaccactg
     5101 tgaccttcgg ctggacggca ggcgcgggtg cgtcggccta ctggctcgat gccgggagca
     5161 ctcccttcgg taacaactac taccagtcgg gcaacttggg taacgtcctg accaccacgg
     5221 tcagcggttt gccgtcggat ggcagcaccg tatacgtcac cctgtactcc tatgtgggag
     5281 gacaatggtt gagcaactcc tacacctata cggcgttcac cccaatcgta accctcgggg
     5341 tcatgcagac gccgattcca ggatcgacgc tgagtggcaa cactgctacc ttcacctgga
     5401 gcgcgggggt aggcgctacg gcgtactggc tggaggtggg cagcagcccg ggtggcaacc
     5461 agtactatca gtcgggaaat ttggggaacg tgctgaccac gacgatgtac actttgccgc
     5521 agaatggcag cacggtctat gcgactctgt actcctacat tgggggacag tggcttagca
     5581 acagctacac ctacggctca gccatggcgt ttcagggttt tgaaaccgac atcggcgatt
     5641 ggcagccgat cacaactcga gtggcctcgg gcggcggcgt tcttggcgtg tcttcggcct
     5701 cgggcagtta tcacgccgaa ctcaccaacg tctacgacac ctacgggaca ggctacggcg
     5761 gcgcagagta tagtttattc ggttacgcga ctcagccccc gtatcccggt gatttttcac
     5821 aatcgatcag catgtacatc gacaagaact ggccgacggc ctcgccgaat ggcaacggcg
     5881 ttggcgtctg gatcgacatg tcaccaggta acccggatcc caacaactac ggggcggagc
     5941 acaatttccg cttcactccg tccggaactg cggtggccgt ttctgtggac ggccaaggca
     6001 gtcccatcgc gaccatcacc acctccggtt ggtacaagtt ccagatgacg taccaaaaag
     6061 gagcgctgcc caccgacctg gtaatcacca atatgaacgt cttcgattcg actgggcacc
     6121 tggtcggaac gacaacggta ctcgccaatt cgccgggggg accactactc agcgagaatc
     6181 tcctcggtcc cggctatgtc tggatcaccg tgtgggcgga cggatggccg gctgcagggg
     6241 cgaacgacgt gcttgccatt gacgatgtcc gcacggacct tctctaattg tctgcgacgg
     6301 gtagggcggt gctttgcaag gaaacctgac tttcaccagg gcacggactt cggttcgtgc
     6361 cctctttttg gcagtgttct ttgtgccgat aacggcgggt agccgtcaaa ccactcagag
     6421 tgggctcagg tcaagggcct cctcagttgg agacggcgat cctcgtcgtg gatgccaaca
     6481 accagagcaa taatgccgga cagtttgagc tgcgcaagga aaaggacggg agcttcgtgg
     6541 tagttggtgt tggggcgcac ggcccaaacg gcgagagttc aagccagcag cctattctcg
     6601 attcgcttat cagtcttaca gcagagcgga gaagtgcgcg tcagacaatt gctctcatct
     6661 gccagaaggt aagccaacag agcaagattc aagttactgc tgacggcgta gccgacagcc
     6721 ttcctgaacc taaaactgtt gcagttggcg gagttggagt accagcccga acgctgctct
     6781 ctcgcacgct ggcatccatg agaggtaagt tgtattggcg cttgatctac gattcaaatg
     6841 agaagagtta ccaattgagc ataagccgtg agtcgcaata aaaaaggtgg acagccagta
     6901 gaaccaatct cttaggcccg gctcctgcag cttccggata gtttccattt ttctaactga
     6961 ccgagcgttg ctaaagtcac tcgcctatgg aactgcggca acacttagga gggtcgccgc
     7021 ctggagcgcg gcttgcagcg tggcgccggc aatgaccttg cccgtactgg aaggcacaac
     7081 gaggatgtcg tcggcttgca tttgcaagtc tggagctttt gcctgcagga ttttctttag
     7141 ctcgacggga gtttccgtca tccctccggg gcccttacgg aggatctttg cgccgctcag
     7201 cttagccgtc cgagttgtgc ctccggccaa agcaaccgcc tgtaatacgg tgagaccgcc
     7261 cgcttccatg agcaatccgc ttggtcttgt cacgtctcct accacgtaaa cgatatccgc
     7321 cttgcgcacg atgatggtgt ccccggggaa gactgggacg ttggtatccg gattatcttt
     7381 aagatttcgc gacagatcga tggtagcagt cttgtcagga tcgctgcgat gagtaacggt
     7441 gaggctgcgg ccagcttttt cgcttagtcc gccggccgct gagatcagat cgaaaagatg
     7501 ttgttgtcca agcaccggat aaacaccagg ccttgcgact gctcccagca cgctcgctcc
     7561 ctccgaggca tagtccgtca cgaaaagaga gacgtgcggg tttttcacga agtcagcgag
     7621 ccgtgtctcg atgagcttct gggcctcttc ggccgtaaga ccagccacgt gtgtgtaccc
     7681 gatgagcggg cagtacatgt caccgttgct ggagatgcga gttttggtgg cgagttcggg
     7741 aacgccataa acgctcattt cgagcaggtc cccaaccccg agctgcaggg acatgtctcc
     7801 gccgcgccga ctagacgaat cgatcccggg ggccgatgag gctttgccct cagcattatt
     7861 actgttatga ccattgttca ggggagcggt ttgatcttgg gctgcagcat ttgcccctga
     7921 cgcacaaaac agagtggcag cgaagaacag cgcaagactg agggttttga atgacttcga
     7981 tggcaacaac ggcatgtact acctctgaca tctacctagg aaccaacagt ttagcatgag
     8041 gaaagctgcg gagaaagaac cagaattcga tgtgatccgc agaaacctta tgcgcattca
     8101 agataggcgg gcccgcacct tttctggtac cgacagtcgc tgccattacg ttagcagccc
//
LOCUS       DASRKK010000135         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_888377, whole genome shotgun sequence.
ACCESSION   DASRKK010000135 DASRKK010000000
VERSION     DASRKK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888377"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFC15_03130"
     CDS             <1..206
                     /locus_tag="VFC15_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM09187.1"
                     /translation="YAKKIGLGQVAPHDLRRSCAKLCHTAGGELEQIQFLLGHVSVQT
                     TERYLGCKQRISGAVNDRIGIEP"
     gene            722..1576
                     /locus_tag="VFC15_03135"
     CDS             722..1576
                     /locus_tag="VFC15_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09188.1"
                     /translation="MSRVLVIGSDSQVSREIGSALSATGFPMEYSAGHADALQRLRMR
                     SFGVVITSPDSTVEEDLALLEEMRAIRPGVKSIVLARHSTSEEVIAALRARVFACFTP
                     PFDPGEIANLACSAASDSQGRDDIQILSARPGWVSVRVNCRLITAERLMTFANELSAR
                     LPEDTRQEMMQAFREILLNAMEHGTAFNPEQVVEVTAVRTARAMVFYVRDPGAGFRRE
                     SLTHAAIANPDDDPAAHIVQREAEGMRPGGYGLLLAGGTVDELIYSDIGNEVLLIKYV
                     DSASLQAG"
     gene            1911..2249
                     /locus_tag="VFC15_03140"
     CDS             1911..2249
                     /locus_tag="VFC15_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM09189.1"
                     /translation="MKASIRQVEGVAILDLSGRIKLGEGSSLLREIVKDLLSKGQKNI
                     LLNLGDISYIDSSGIGELVSAFTSVRNQGGELKLLHLTKKVHDLLQITKLYTVFDVKD
                     DEAAAIGAFV"
     gene            2485..5406
                     /locus_tag="VFC15_03145"
     CDS             2485..5406
                     /locus_tag="VFC15_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM09190.1"
                     /translation="MIGTTISHYRILEKLGGGGMGVVYKAEDIRLSRFVALKFLPLNV
                     AHDPNALARFRREARAASALNHPNICTVHDIGEQNGNAFIVMEALDGVTLKHYIAGKP
                     MEPELIVSLGVEIADALDAAHAAGIIHRDIKPANIFVTKRRHAKILDFGLVKLTTAQE
                     VSGSSETAVVRPDEPYLSSPGAMTGTVAYMSPEQVRAQEVDARTDLFSFGAVLYEMAT
                     GQMPFRGSSSGEICGAILHEQPRPPSQLNPALPERLEEIIHRALEKKRELRYQHASEM
                     RAELERLKRDWDASSSITSTPGKRPSLSSGSSSFPASSAAMQVAPVASQKRTATAAMG
                     RSWKLLLPVLSVAVLVAAMIAWFFYRHSTRNSSQAGKNTIVLADFTNTTGEAVFDGTL
                     KQALTIQLEQSPYLKLLSEQKVRSTLKLMDKPPDTRLSNETAREVCQRSNSNGVISGA
                     IDSVGNHYLISLRAVDCQSGDTLASAKAEADSREAVLRRLGDAGDELRRKLGESLASV
                     QRYNKPLDQATTSSLEALQAFSEGRHMQWLKGDSASIPFHKRAVELDPNFARAYAALG
                     MAQNNLGEYSAATESFTKAFQLRDRVSDRERFYIEAAYYSFATGELLKGIETYRQWSA
                     VYPEDFIPYANLPLDLEPLGEYEKAVESARQAIRLNPDSGAAYGALVSGYVYLDRLDE
                     AKAMYEQAIARHPDIEYLHRQRYYIGFLEQDEAAMLKQVAWAAGKPGESQLGWEEAQT
                     AAYHGELTKARTLSQTAELQAKAADNPEQAALIRVLFAGIEAEFGNPGVARQQVSAAL
                     ALNAGRDEMVAAGLALGRAGDAGQAQKFADKLNQQFPLDTIIQLYWLPTIRACIALQR
                     NDPRQAIAALEAAVPYELGNQGYGLLYPVYLRGMAYLQAKQDERAAAEFNKLLTRRGL
                     MKNSPLAALAQLQLGRAQAMSGDTPAARKSYQDFFVTWKNADPDISVLLQAKAEYGKL
                     Q"
BASE COUNT         1216 a         1585 c         1652 g         1130 t
ORIGIN      
        1 attatgcgaa aaagatcggt ctgggccaag ttgccccaca cgaccttcgt cgttcgtgcg
       61 cgaaactatg tcacacagcg ggcggagaat tggaacagat tcaattcctc ctcggccacg
      121 tttcggttca gactacggag cgctaccttg gttgcaaaca gcggatcagc ggagcggtca
      181 acgaccggat cggtatcgaa ccgtagcgcc gagggtaatg ggcgacccac tagcgcagcg
      241 aggtgccgca ccaggggagc gacttggcga ctattcgctc aagtggattt ccgccgcgaa
      301 attcgcccaa tagcattcgt agaccctggg gcgtttctcg ttcctaatcc ggccagatct
      361 cccacaagaa gacttcggga caactttcgg agccagcctc cggtgtgggt cgctgtttga
      421 gcgccgataa ctggcggttt gtccgtcact cggtcggaat cgttcctccg cgtcgcctta
      481 tggttgccgc cgatcaactt gtgctggtga tttcttcggc gactcactcc gtcaggtgat
      541 gggcctccac gacgcgctct tctagtccgg actacgccca tttgacagct tcctaaacta
      601 tggcaaggtt gatcgccagg caactccgta tggacaaggc gcaaatgcca gctcttgatc
      661 tatgatctgt ctacacaagt tccgcagttg atttgctgag ttcattgcct cggagcacaa
      721 catgagccgg gtcctagtca ttggaagtga cagccaagtg agtcgggaga taggcagtgc
      781 actctctgcc accggcttcc ccatggaata ctcggcaggg cacgccgatg ccctgcagcg
      841 gttgcgcatg cgatcgttcg gggtcgtaat caccagtccc gacagtactg tcgaggaaga
      901 cttggcgcta ctggaggaga tgcgcgccat ccggccgggg gtaaagagca tcgtcctggc
      961 tcgtcacagc acttcggagg aagtcattgc tgccctgcgt gcccgagtgt tcgcctgttt
     1021 cacacctccc tttgacccgg gtgagatcgc gaacctggcc tgcagcgcag cctccgacag
     1081 ccaagggcga gacgacatac aaattctgtc ggccaggcct ggctgggtat cagttcgcgt
     1141 aaattgtcgc ctcatcacag ctgagcgtct catgacgttt gcgaacgagt tgagtgctcg
     1201 attacctgag gacacgcggc aggaaatgat gcaggcgttt cgagagattt tgctaaacgc
     1261 aatggaacac ggaacggcat ttaatcccga gcaggtggtt gaggttaccg ccgttcgcac
     1321 tgcaagagcc atggtgttct acgttcgtga ccccggggct ggcttccgcc gcgaatcact
     1381 tacccatgcg gctattgcaa atccggatga cgatcccgcg gcccacatcg tgcagcgcga
     1441 agcagaagga atgcgcccgg ggggatacgg ccttcttctg gccggtggca ctgtggatga
     1501 gttgatttac agtgatatcg gtaacgaggt gctgctcatc aagtatgtgg attcagcttc
     1561 gctgcaggcg ggatagatcc tgacggcgtt atcgttggcg tctgtttgga cgccgcgggc
     1621 cgttactcgg gcagagtacc gattgtcacc caatttgttg caggcactcc ttctagttga
     1681 agccgacatt tcagataagc ggcttcggga tgcccccaac cggaatctcc ggttgtcacg
     1741 cctgtatgtt caaatgtcac agacaaaccg ccgtccgagg gtttgaaatt ctcatggctg
     1801 ccagggggag acgcgtgtca cttctttcca tccgctctcc gcactgaaat taccttgatc
     1861 aagtgattcc agaacaatga actggaccgc cgggaggctt ccacgtgagc atgaaagcga
     1921 gcattcggca ggttgagggc gttgccatcc tagacctcag tggacgtatc aaactaggcg
     1981 agggcagctc gcttttgcgc gagatcgtca aggacttact gagcaaggga cagaaaaaca
     2041 tcctgttgaa tttgggcgac attagttaca tcgacagctc gggcatcgga gagctggtca
     2101 gcgccttcac ctctgtacgc aaccagggcg gcgaattgaa actgctgcac ctaaccaaga
     2161 aagtgcacga tctgctgcag atcaccaagc tctacactgt cttcgacgtg aaagacgacg
     2221 aagcggcggc tatcggggca tttgtttagt cccaaggtaa gtgtcgggtg gcccgttttc
     2281 cgagcggcat ggcgggagtg ctccaacggt gtgggaaggc tgtcgcatcc agacgtggca
     2341 actaaccagc caagtcgaac cgcacaggcg cgattacctt atgacaaggg tcttccggca
     2401 caaggcagca gcagccaaat ggattgactt gcttacactc tgcccctacc atcaagtgca
     2461 aacccagttc tgtcaatctg tctaatgata ggcacgacga tctctcacta ccgcatactg
     2521 gaaaagcttg gcggtggtgg tatgggagtg gtgtacaaag ccgaggacat acgcctcagc
     2581 cgcttcgtag cgctcaagtt tctccctttg aatgttgcgc acgatcccaa cgccctggcc
     2641 cgcttccgcc gcgaagcacg ggccgcctct gcgctgaacc atcccaacat ctgcactgtt
     2701 cacgacatcg gcgagcagaa cggaaacgct ttcattgtga tggaagcgtt ggacggcgta
     2761 acgctcaagc attacatcgc gggcaaaccc atggagccgg agctgatcgt ctccttgggg
     2821 gtcgagattg ccgacgccct ggacgctgcc catgccgcgg gcatcatcca ccgcgatatc
     2881 aagccggcca acatcttcgt caccaaacgc cggcacgcca agatactgga cttcggatta
     2941 gtcaagctga cgactgctca agaagtgtcc ggcagctctg agactgcggt cgtgcggcca
     3001 gacgagccgt acctcagcag cccgggcgcg atgacgggaa cggtagctta tatgtcccct
     3061 gaacaggtac gggcgcagga ggtggatgcg cgcacggatc tgttctcatt tggggcggtg
     3121 ctgtatgaga tggctacggg ccagatgccg ttccggggca gcagttcagg ggagatctgc
     3181 ggggcgattc tgcacgaaca acctaggccc ccgtcccaac tgaacccggc cctcccagaa
     3241 cggttggagg agatcatcca tcgagccctg gaaaagaaac gcgaactgcg ctaccagcac
     3301 gcctcggaga tgagggccga gttggagcgg ttgaagcgcg actgggacgc gtcgtcaagc
     3361 attacaagca cacctggcaa gcgaccttcc ctgtcttcgg gttcttcttc gttcccggcc
     3421 tcctcggctg ccatgcaagt tgcgcctgta gcttcgcaga aacgcaccgc gaccgccgcg
     3481 atggggcgaa gctggaaact actgctgccg gtcctgtcgg tagcggtact tgtagccgcc
     3541 atgatcgcgt ggtttttcta ccggcactcc acgcgcaatt cctcgcaggc gggaaaaaac
     3601 acgattgtcc tcgccgactt caccaatacg acgggcgagg ccgtcttcga cgggacattg
     3661 aagcaggcgc tgaccattca actggagcaa tctccttatt taaagctgct ttcggagcag
     3721 aaggtccggt ctacgctcaa gctcatggac aagccgccgg acactcgcct gagcaacgaa
     3781 accgctcgtg aagtatgcca gcgcagcaac agcaatggtg tgatcagcgg tgcgatcgac
     3841 agcgtgggca accactatct aatcagcctg cgtgcggtcg attgccagag cggcgacacg
     3901 ttggcaagcg ctaaggcgga agccgatagt cgagaggcgg tgctgaggcg actgggagac
     3961 gccggcgatg agttacgccg gaagctgggt gagtcactcg cctccgtgca gcgctacaac
     4021 aaaccactgg atcaggcgac aacgtcctca ctggaggcac ttcaggcctt ttctgaaggc
     4081 cgccatatgc agtggctaaa gggagattcg gcatctatcc cgtttcataa gcgcgccgtg
     4141 gaactggacc cgaatttcgc tcgcgcatac gcggccctgg ggatggccca gaataacctc
     4201 ggcgagtaca gcgcggcgac ggaaagtttc accaaggcct tccagctacg tgaccgcgtt
     4261 agcgatcgcg agcgcttcta catcgaagcc gcctattaca gcttcgcgac cggcgagttg
     4321 ttgaagggca ttgaaaccta tcggcaatgg agtgcggttt acccggaaga cttcattccg
     4381 tatgccaact tgccgctgga tctggaaccg ctgggcgagt acgagaaggc tgtcgagtcg
     4441 gcccggcagg ctatccgatt gaatcccgat tctggcgctg cttatggcgc gcttgtgagc
     4501 ggttatgtct accttgaccg gctggacgaa gccaaggcca tgtacgaaca ggccatcgcc
     4561 aggcaccccg acattgaata cctgcaccgg cagcgttatt acattggttt cctagaacag
     4621 gacgaagcag ccatgctgaa gcaggtcgcc tgggccgcag gaaagccggg tgaatcgcag
     4681 ttgggttggg aggaggcgca gactgccgcc taccacggag aactaaccaa agctcgtacc
     4741 ctttcgcaaa cggcggagct gcaagccaag gctgccgata acccggaaca ggcggcgttg
     4801 atcagggtgc tctttgcagg tatcgaagcc gagttcggca atccgggggt cgcgcgacaa
     4861 caggtttcag cagcgctggc cctgaatgcc ggcagggatg agatggttgc cgccggatta
     4921 gcgctggggc gcgcaggaga tgctggtcag gctcagaagt tcgcagacaa gctgaaccag
     4981 cagttcccac tggatacgat cattcagctc tattggctgc cgaccatccg ggcatgcatc
     5041 gcgttacagc gaaacgatcc gcgacaggcc atcgccgcat tagaagccgc agtgccttac
     5101 gaattgggga accagggata tggtctgctg tacccagttt atttgcgtgg aatggcatat
     5161 ctgcaggcaa aacaggacga acgggctgcg gcagaattca acaagctcct aacgcgtcgc
     5221 ggtctgatga agaattctcc cctagcagcc ttggcgcaac tgcagctcgg ccgcgcacag
     5281 gcaatgagcg gcgacacccc cgcggcgcga aagagttacc aggatttctt cgttacttgg
     5341 aagaacgcgg atcccgatat ttccgtactg ctccaggcga aagccgagta cggcaagctg
     5401 caataacgat cacgctagat ccgtaatctc atgtgcggtt cagaaacctt agtgctgtat
     5461 cggacgcgcc ccaaacacag tgagtgtgca ggtaggctcg gcaccgcgag gcgcgtccga
     5521 tacagcggtt tgaaggaagc cgctcctggg gaggcgatta tgcctattca atcgcgttgg
     5581 ggt
//
LOCUS       DASRKK010000136         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_106466, whole genome shotgun sequence.
ACCESSION   DASRKK010000136 DASRKK010000000
VERSION     DASRKK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106466"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFC15_03150"
     CDS             <1..190
                     /locus_tag="VFC15_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09191.1"
                     /translation="GALSYLTLWPDGTGQPLVSTLNAQDGSISSNMAIVPSTNGKVDA
                     FASGITQLILDISAYFAP"
     gene            complement(775..1164)
                     /locus_tag="VFC15_03155"
     CDS             complement(775..1164)
                     /locus_tag="VFC15_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09192.1"
                     /translation="MMRQIFFLLLFSSTLCAQSAGGQPIPLAQNGQSQADAAGQSEMR
                     PSPGEAGDDLAQMRTDLNHMESLLNNMSSEITFLRDQNLQILLNSNARMWTILIRDLR
                     LQIDRQEQRRTGKRAPSAAGPQVRQNH"
     gene            1406..2365
                     /locus_tag="VFC15_03160"
     CDS             1406..2365
                     /locus_tag="VFC15_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZM09193.1"
                     /translation="MPTQAKLNSAPRRDEREMRDARFAMRLSLMFGLAMLIGKTTAYL
                     MTHSAAIFSDAAESVIHVIAVGFAGFSLRLSTKPASPHFLFGYERITFFSAGFEGAMI
                     VVAAIAILFESVRRWIAGLQLEHLGTGVLLILMAGILNAGLGYYLLRTGRRTNSLILE
                     ANGKHVLTDSWTSFGVVGGLGLVLLTHWKPFDPLVAIAVAVNILWSGGHLVWRSAVGL
                     LDYSDPEAGRKIRDKLDTICSELSIQYHGVRFRTTGYRQIIEVHLLFPRVTSVSEAHR
                     LATMLEERLPAELGMPAEVMTHLESLEDHADVHHEQHYTGKPE"
     gene            2514..>2705
                     /locus_tag="VFC15_03165"
     CDS             2514..>2705
                     /locus_tag="VFC15_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09194.1"
                     /translation="MHRITLGDFELTVLSDGTYRMDGGAMFGVIPKVMWEKRIQPDER
                     NTIPLALNSLLVRTGKQNIL"
BASE COUNT          548 a          802 c          752 g          603 t
ORIGIN      
        1 aggcgcactg agctacctga cgctgtggcc ggacggcacc ggccagcctc tggtttcgac
       61 gctgaacgca caggacggat cgatctcctc caacatggca atcgtgccca gcaccaacgg
      121 aaaggtcgat gcgtttgcgt ctgggatcac gcagctgatc ctcgacatct ccgcctactt
      181 cgcgccgtag ggtgcttcga accgtctcgt acgggtagtg cgggcctgaa ggcccgcact
      241 acccgatcac ctgcactcgt gggcttggcc ggaaaggctc gcccaatcgc catcgcaatc
      301 tcggcgcctg gtgccgaatt gcggctcatc cttgcggcgt cgttcgactt cggtctcccg
      361 cgtgaacaag tcgtatccat cgtcttccgg caggtcgcac tcatctctga cattcaacat
      421 ggcagtcatc tcgcagcttt gctattacca tggtcgcctc ttcggcagcg acgcgcagtg
      481 acgcagcgca cgtaagcggc ggtagacgtt cccgaagctg cactgttgtg atttgcaaca
      541 ctcagggcca tgctgcgtga ggtcagtgat tgtctcagaa tcgccaaagt ggactaaatc
      601 aatggaaact agctagcaat ttcgcgcatt aaccaattct tggtgctcca tgactagtaa
      661 agtgggaaat agattcgcat tagacgagta atagcacgct tctcgcgcac acgctgtagt
      721 ccgggccgct ctcatgatga caaccctgat gtgtgctacc ctgttctgcg aaaatcaatg
      781 gttttggcga acctgcggcc cggctgcgga cggcgccctt ttgccggttc ggcgctgctc
      841 ctggcgatcg atctgcagcc gcaggtcgcg aatcaagatc gtccacatgc gggcgttgct
      901 gttcagcagg atttgcaaat tctggtcccg cagaaacgtg atctcgctgc tcatattgtt
      961 gagcaggctc tccatgtggt tgaggtcggt gcgcatttgc gccagatcgt cacccgcttc
     1021 gccaggggag ggccgcatct cggattgccc cgcagcgtct gcctgacttt gcccgttctg
     1081 agccagcgga atgggctgcc cgccagcgct ctgggcgcat agtgtcgaac tgaacagaag
     1141 tagaaaaaat atttgccgca tcatcgccgc acccctccta gagtgtaata gtttgaactc
     1201 gaccttgggg acagcgcctg cgagttacca gttgggagag accggacaag cattccgggt
     1261 ccgaggtccc aacttcccga ttgccgaccc ctcgttcaga atcgccaacc cgccgggctg
     1321 gtgcgtgagc cacatcacat cttccagtgc gatttctgtg ggactatgtg ccaaacacta
     1381 agcttctgat ccagagatac agagcgtgcc tacgcaagcc aagcttaact ccgcaccacg
     1441 aagagacgaa cgggagatgc gtgatgcgcg cttcgccatg cgtctctccc tgatgtttgg
     1501 tttggcaatg ctgatcggca agaccacggc ctatctcatg acgcattcgg ctgccatttt
     1561 ttccgacgcg gccgagtcgg tcatccacgt gatagccgtg gggtttgctg gttttagtct
     1621 tcggctcagc acaaaaccag cttcccctca tttcctcttc ggttacgaac ggatcacctt
     1681 cttttccgct ggatttgaag gagcaatgat cgtggtggcc gcgatcgcaa ttctgtttga
     1741 gtccgtccgg aggtggatcg ccggactaca actggagcac ctgggaacag gcgtattgct
     1801 gatcctgatg gctggaattc tcaatgcggg tctcggttac tatctcctgc gcaccggccg
     1861 gcggaccaat tcactcattc tcgaagccaa tggcaaacac gtcttgaccg atagctggac
     1921 cagctttggc gttgtgggag gactagggct ggtgctactg acgcactgga aaccgttcga
     1981 tccgctggtg gccatcgcgg ttgccgtaaa tatcctctgg tcaggaggcc acttggtctg
     2041 gcgttcggca gtcgggcttc tggattattc cgatccggaa gccggtcgga aaattcggga
     2101 caagctggat acaatttgct cggaactgag cattcaatac catggcgtac gcttccgaac
     2161 caccggatac cgccagatca tcgaagtaca cttgttgttc ccgcgcgtga cgtcggtcag
     2221 cgaggcccac cgcctcgcta ccatgctgga agagcgcctg ccggccgaac tagggatgcc
     2281 cgccgaggtc atgactcacc tggagtcctt ggaagaccat gccgatgtcc accacgaaca
     2341 acactatacc gggaaacctg agtgaagggc agcacgatgg gtccttcagg tcatcgtgtt
     2401 gaggatcacc gcagccagat aatcgccgtc agataggccc cctccgcccg ctttgtagcc
     2461 tgccgtgctg attcagtatg atcggaaaac caacttcttg tggagacaag ccagtgcacc
     2521 gaatcacgct aggcgatttt gaactcacag ttctttccga cgggacctac cgcatggatg
     2581 gcggcgccat gttcggcgtc attcccaagg tgatgtggga aaagcgcatc cagcccgacg
     2641 agcgcaacac cattcccttg gcgctcaatt cgctgctggt tcgcaccggc aagcagaaca
     2701 tcctc
//
LOCUS       DASRKK010000137         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1377071, whole genome shotgun sequence.
ACCESSION   DASRKK010000137 DASRKK010000000
VERSION     DASRKK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377071"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFC15_03170"
     CDS             complement(<1..524)
                     /locus_tag="VFC15_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZM09195.1"
                     /translation="MNVISWNLLRLTGAGVEDVAALVERHHPDLLFLQEAIEEMADLP
                     ALVGGYFFREPLEGRVYGLAVWSPHPLLRPYALRLPVSQVPGRVPPRVAQIVHLDDVA
                     FANVHLSHGQFLNRWQLVHIANALDGPAAIVGDYNAVGPIKLAGFKDVGPRQSTHSPN
                     DIISFRLDRCMARG"
     gene            complement(817..1263)
                     /locus_tag="VFC15_03175"
     CDS             complement(817..1263)
                     /locus_tag="VFC15_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZM09196.1"
                     /translation="MKWDVKSIERHLFGAGEALFQTGWFMESITTQVLVVFAIRTRRP
                     IFRSRPHMFLAAMALGVIAVAIALPLLPVRRWLGFVAPPPLFFVFLIEQLWQLAARYD
                     KVNRHIHRIESEEEPTADGVLEGLKKQRLKLKDDIATQLGKLERRM"
     gene            complement(1355..>2198)
                     /locus_tag="VFC15_03180"
     CDS             complement(1355..>2198)
                     /locus_tag="VFC15_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM09197.1"
                     /translation="GHTAVIADYYDGQSLVQTLLANGIDHVALTDWKSATPDMKDLEV
                     DNYLAEIIVAIDDLGGRVNLVGLCQGGWMSAMIAARFPDKVNSLVLAGAPIDTDAGNG
                     SIKKMVHQSPASFYEELVALGGGLMTGKFMLQGWKNMHPEQHYIQDHVDLYEHIDDPA
                     YLAKEETFESWYENPIDLPGRWYLQVITQIFKENRLAKGEFMGLGRKLNLHDITCPTY
                     LLAGADDDITTPEQVLDAAKYIGTAKDHIVQKTVPGGHIGLFMGANTLREHWPQIARW
                     IGAQ"
BASE COUNT          476 a          644 c          633 g          445 t
ORIGIN      
        1 aaacctcgcg ccatgcaacg atcgaggcgg aaggaaatga tgtcgttcgg actgtgtgtg
       61 gattggcgcg gcccgacatc cttgaagccg gcaagcttga tcggcccgac cgcgttgtag
      121 tcgccgacaa tggccgctgg accgtcgagg gcatttgcga tgtgcaccag ttgccaccgg
      181 ttcaggaact ggccgtgcga aagatgcacg ttggcgaaag ccacgtcatc caggtgcacg
      241 atttgtgcga cgcgaggcgg cacgcgaccg ggcacctgcg aaactggcag gcgtagcgca
      301 tagggccgca gcaatggatg cgggctccac acagccagac cgtaaacgcg gccttccagt
      361 ggttcgcgga aaaaatatcc accgaccaag gccggtagat ccgccatttc ctcgatcgct
      421 tcctgcaaga agagaaggtc gggatgatgt cgttcgacga gagcggctac atcctctacc
      481 cccgcgccag tcaagcgcaa caagttccag cttatgacgt tcaagtttct attcccggct
      541 ggcgatcttc agttcccaag tatcccagtt tcccgaatga cactcgaagt caggcggagt
      601 gccgcggcgc cccgctggta ctaagactgt agtcgctcta aaatgggagt ctaggcgaga
      661 tttagtgaga cggacgagcg attcaaggcc gctacgatga aagaatccaa ccacatacgc
      721 gacaccggcc aaggtcgacg ctagaatatt acacgcctat ctctagcgag ggttcgggca
      781 accggcacac cgagctggaa caccgcgccc gggagcctac attcggcgtt ccagcttccc
      841 cagctgggtt gcgatgtcgt ccttgagctt gaggcgctgc ttcttgagcc cttcgagcac
      901 cccgtcggcg gtcggttctt cttccgactc aatgcggtgg atatgtcgat tgaccttatc
      961 gtagcgggcc gcaagttgcc ataattgctc gatcaggaag acgaagaaca gcggcggggg
     1021 cgccacgaac cccaaccatc ggcggaccgg gagcagcggt agagcgattg cgacggcaat
     1081 aaccccaagc gccatggcgg cgaggaacat atgcggtctg ctccgaaaga tcggccgtcg
     1141 cgtccggatg gcgaagacta caagaacctg ggtggtgatc gactccatga accagcccgt
     1201 ctgaaacagc gcttcgccag cgccgaacag atgccgttcg atggacttga cgtcccattt
     1261 cattggcccg gcgatagcat cttcatcgac tcctgcacac aatgaggcaa gaccgaggac
     1321 tgcgcggttt ggaatatcgt aacgatgaaa ccatctactg ggcgccgatc caccgggcga
     1381 tctgtggcca atgttctcta agcgtgttcg ctcccatgaa cagccctata tggccgcccg
     1441 gcacagtctt ttgaacgata tgatcctttg ctgtcccaat atattttgcg gcatcaagga
     1501 cttgctccgg cgtcgtaatg tcgtcgtctg cacccgccaa caaataggtg gggcaagtga
     1561 tatcgtggag attgagcttc cggccgaggc ccatgaattc gcctttggca aggcgattct
     1621 ccttgaagat ctgggtgatc acctgaagat accagcgtcc cggcaggtca atcggatttt
     1681 cgtaccagct ctcgaaggtc tcttccttcg cgaggtatgc cgggtcgtcg atgtgctcat
     1741 agagatcgac atgatcctga atgtagtgct gctcggggtg catgttcttc cagccctgca
     1801 gcatgaactt gcccgtcatc aagcccccgc caagcgccac cagctcctcg taaaacgatg
     1861 cgggggattg gtggaccatc tttttgatcg agccatttcc ggcatccgtg tcgatgggcg
     1921 cgcccgccag caccagtgag ttgaccttgt cgggaaaacg cgcggcgatc atcgcggaca
     1981 tccagccgcc ctgacaaagc ccgacaagat tgacccgtcc gccgagatcg tcgatcgcta
     2041 caatgatttc ggcgaggtag ttgtcgacct ccaaatcctt catatccggg gtggccgact
     2101 tccaatcggt aagcgccaca tggtcgatgc cgttggccag gagagtctgc accagactct
     2161 gaccgtcata atagtcggcg atcacggcgg tatgaccg
//
LOCUS       DASRKK010000138         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_790939, whole genome shotgun sequence.
ACCESSION   DASRKK010000138 DASRKK010000000
VERSION     DASRKK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790939"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..169
                     /locus_tag="VFC15_03185"
                     /pseudo
     CDS             83..169
                     /locus_tag="VFC15_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            193..372
                     /locus_tag="VFC15_03190"
     CDS             193..372
                     /locus_tag="VFC15_03190"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZM09198.1"
                     /translation="MTLSIACKDVGMKNCDFKAEASTEAELLKKVAQHAKDVHGMVTV
                     DAATLAKVKSVIKKV"
     gene            complement(704..979)
                     /locus_tag="VFC15_03195"
     CDS             complement(704..979)
                     /locus_tag="VFC15_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09199.1"
                     /translation="MPNPETVEHPFGTIKARMGATHFLMKTLSRVAAEMALHVLAHDR
                     CNPHQPRLQSQKPSNPKIMTAHGYLAEFAPRLCLCQAFSHDQDPKQT"
     gene            complement(1123..1734)
                     /locus_tag="VFC15_03200"
     CDS             complement(1123..1734)
                     /locus_tag="VFC15_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZM09200.1"
                     /translation="MSISKEDHERIARAIHSAEAKTSGQIVCVLAQASSHTTALPVFI
                     AAVASLVMPWLLITFTAMTVHRILSLQSVVFLALLVLFCLPRVRIALMPRKARRAIAH
                     RAAMEQFNSRGIGQRKDRSGILIFVSLAERYARIIADDEIAARVAQSEWQAAVNALVA
                     HMRDGRIADGFVTAINLCGNELAKHFPRTATSNNAQPDRIYLI"
     gene            complement(1734..2513)
                     /locus_tag="VFC15_03205"
     CDS             complement(1734..2513)
                     /locus_tag="VFC15_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZM09201.1"
                     /translation="MTPGRTASLLARGGLRVVVGFIAFVCVGIAAFAVNYPALTGRIV
                     DQAKIISSDTRSAIEPKLIDLEAKSGIQLVVATVTSLEGQEIEPYANELFRIWKLGEK
                     EKNNGVLLLVAPNEHRVRIEVGYGLEGTLTDALSKVIITNAITPRFKTGDFSGGISRG
                     VDDIITVLTTDASEWQQRPSLRLDNQQTTDPTSWLLIAALIVFVTLLTVSPGFRWLFL
                     SLVLNILVSSGGLSGRGGFSGGGSFSGGGGSSGGGGASGSW"
     gene            complement(2542..3141)
                     /locus_tag="VFC15_03210"
     CDS             complement(2542..3141)
                     /locus_tag="VFC15_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZM09202.1"
                     /translation="MTTFISRLRTITAVVFLGLSVSACGYNSIPTYEEQAKARWADVQ
                     NNYQRRADLIPNLVATVQGYAKQEKDVLTAVIEARAKATQVKIDVSQLTDPDKLKQFQ
                     DAQNQLSGALGRLLAVSENYPDLKSNQNFLALQSQLEGTENRITVARRDYIEAVRVYN
                     TSLKTFPGILWAATFFRSNKPMVEFTANDNAQTPPQVKF"
BASE COUNT          808 a          981 c         1035 g          743 t
ORIGIN      
        1 cgcaccgggg gtagacggtc aggacttcgc ggacatcgag gcgtatggcg cagagcagtg
       61 gctcggcgaa ctggcgcttg cgctcaggca ggaaacctac cgaccggacc ctatcagaag
      121 agtgtacata ccgaaggcca acggcaaact caggccgctg ggcatagcgc gtgagatcaa
      181 aaggaggaga tcatgactct cagcattgcg tgcaaggatg tcggcatgaa gaactgcgac
      241 ttcaaagctg aagcatccac ggaagcggaa ctcctaaaaa aagtggctca acacgctaag
      301 gacgtacacg gtatggtgac cgtagatgct gccactttgg caaaagtgaa gagcgtcatt
      361 aaaaaggttt aggcgaagaa ctcaaaactt ggccgccaac tgaggcggcc tagttacgta
      421 ggtttgtcgc cctttggctt ttgcgcttga gtgatcgaca ctccgcaggg tctacactca
      481 aatatctcct gatccgcgct cggccatcgc caaatcgtgc ggacatgctt catggagttg
      541 ccacaagctg ggcagagcgc tgatgggttc ggagattgcg aatagggcat ccataaacca
      601 taccgtctgc ccccgtcccg ccaactcgcc ttcgcctctc acgttcctat tgctgtacac
      661 acgttatcgc gatggaactt tgtaagcgcg agcggcgccc tttttatgtc tgttttgggt
      721 cttggtcgtg tgaaaacgcc tgacagaggc atagacgcgg cgcgaactcg gctaggtagc
      781 catgcgcggt cattattttt ggatttgacg gcttttgcga ctgtaggcgc ggctgatgcg
      841 gattgcagcg gtcgtgggcc agcacgtgca acgccatctc ggcggcaacc cgtgacagcg
      901 tcttcatcag gaagtgggtt gcgcccatcc tggccttgat cgtgccgaag ggatgctcaa
      961 ccgtctcggg atttggcact cttttttaaa ttcacatcac gtcgcgagca atcgctcccc
     1021 gcctagcggg ggctcagaac gtttcggtag aattccgggc aaacgaacca gaccattgag
     1081 cgcaaagtca gcgcggatga caccgacctg acaacgttgg cgtcagatga ggtaaattcg
     1141 gtccggctgc gcattgttgc tcgtcgcggt gcgtgggaag tgctttgcaa gctcatttcc
     1201 acacaagttg atcgccgtaa caaagccatc ggcgatgcgg ccatcacgca tgtgggcaac
     1261 cagcgcatta acggccgctt gccattcgga ttgggccaca cgtgcagcta tttcatcgtc
     1321 ggcaatgatg cgcgcataac gctctgcgag cgagacgaag atcagaatcc cagagcgatc
     1381 ttttcgttgc ccaatcccac ggctgttaaa ctgctccatg gcagcgcgat gagcgatggc
     1441 gcggcgcgct ttacgtggca ttagggcaat ccgtacgcgc ggtaggcaga aaagcactaa
     1501 tagcgccaaa aatacgacgc tctgcagcga tagaatgcgg tgaactgtca tcgctgtgaa
     1561 ggtgatcagc aaccagggca tcacgagcga agctaccgcg gcgatgaaga ccggcaatgc
     1621 cgtggtgtgg gaggatgcct gtgccagcac gcaaacgatt tggcccgagg tctttgcctc
     1681 ggcagaatgg atggccctcg ctatgcgttc gtgatcttct ttcgaaatgc tcatcaccag
     1741 ctccccgaag ccccgccgcc acctgacgac cccccacctc ctgaaaaact gccgccaccc
     1801 gaaaatccgc cgcggccgct caatccgccc gagctgacaa ggatattcag caccagactc
     1861 aggaacaacc atcgaaaacc tggcgaaacg gtaagcaaag tgacgaaaac gattagtgcc
     1921 gcgatgagga gccaggatgt tggatcggtg gtctgttgat tgtcgagccg cagcgaaggg
     1981 cgctgctgcc attccgaggc atcggtagta agcacagtaa tgatgtcatc cacgccgcgt
     2041 gaaattcccc cgctaaaatc gccggttttg aagcgtggcg taatggcgtt agtaataatt
     2101 actttcgaca gcgcatcagt gagcgtaccc tcgagtccat aaccgacctc gatacgcact
     2161 cggtgctcgt tcggcgcgac gagcagcaac acgccgttat tcttttcctt ctcgccaagt
     2221 ttccagattc ggaataactc gttcgcatag ggctctatct cctggccttc gagcgaggtg
     2281 acggtagcaa cgacaagctg gataccggac tttgcttcga gatcgatcag cttcggctca
     2341 atggcgctgc gcgtgtcgga cgaaataatc ttcgcctgat cgacaatgcg gcccgtgagc
     2401 gcaggataat tgaccgcgaa ggcggcaata ccaacgcaaa cgaatgcaat aaagccaacg
     2461 accacccgca ggccgcccct tgcgaggagg gacgcggtgc gacctggggt catttcagca
     2521 ccttcggcct accaggaggt atcagaattt cacctgcggc ggggtctgcg cattatcatt
     2581 tgcggtgaac tctaccatgg gcttgttgga gcggaaaaat gttgcggccc acaatatgcc
     2641 ggggaaagtc ttgagcgagg tattgtagac ccgcaccgcc tcgatgtagt cgcgccgcgc
     2701 aacggtaatc cgattttcag tgccttcgag ctgcgattgg agcgcaagga agttctgatt
     2761 tgacttgagg tcgggataat tctcgctaac agcaagaagc cggcctagag cgccagagag
     2821 ctggttttgc gcatcctgaa actgcttcag cttgtcggga tctgtgagct gcgagacatc
     2881 aatcttcacc tgcgtcgctt tggcccgcgc ttcgatcacg gcggttagca cgtccttttc
     2941 ctgtttggcg tagccctgca cggtggcgac aagattgggg atgaggtccg cgcggcgctg
     3001 ataattgttc tgcacgtcgg cccatctcgc tttggcctgt tcctcgtagg tcgggatgct
     3061 gttgtaaccg caggctgata ccgacaagcc gaggaacacg acggcggtaa tggtacgaag
     3121 gcgcgatata aacgtggtca tgaaggctcc tgctcggcgc tagattaggc gacgttacgg
     3181 tggcggataa caagctcgac ggtggattat agagtttctg acttcagggt cacgcagccg
     3241 ataagaataa ggcacatata gccccgcagc gcccaaaaag ggtaggcgcg tactcataag
     3301 cacggctcag cagcaagggt gccccgaagc gaattgattc tgcgcgcctt tcctgattcg
     3361 ccaagccgtg atcgatgaga taatgtcgcg tgaggggccg gacggctacg tcaagatggc
     3421 ccaacgccca ggacgggtgc ggttccgcaa ctgtgggtaa gtgaataagt tagtcgctca
     3481 tgcgtacctt tgttcaacca ccaccctcgt cgcactgcaa ccaatgcggt ggcgagttgc
     3541 gactccagca gattgagcgc gccaacg
//
LOCUS       DASRKK010000139         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_9062, whole genome shotgun sequence.
ACCESSION   DASRKK010000139 DASRKK010000000
VERSION     DASRKK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9062"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..580
                     /locus_tag="VFC15_03215"
     CDS             38..580
                     /locus_tag="VFC15_03215"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HZM09203.1"
                     /translation="MALLHTGDRICASCGGMNGKHRPGCIGDDTVEVISTYGRQQAIE
                     DGILVDCEQAPMGEMRRQLIKWPLAMTATAFHRYVWPIDEKANLPPGQSLEGRFWDVV
                     WMFHAAVKGTVPARRIDPCNLLFDFYCIVADTALWPNERIDATARCGPEGMRLVTLKA
                     VSGPGDDGEPVMTFMLPEED"
     gene            809..1507
                     /locus_tag="VFC15_03220"
     CDS             809..1507
                     /locus_tag="VFC15_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09204.1"
                     /translation="MDVGHFKRARKQAGLTQKRAAAKLRVSQSYLSMVENGQRALPPE
                     LARMMVHVYKLSPVSFPPSETRWDPKRADPDQLAADLAGLGYPGFAYLRKRRSERNPG
                     ELLLTALAQDRLEARLFEALPWLVLKYWEMDTGWLVQEAKLHDLQNRLGFVVSLARTA
                     GNRVALSNPERDAALEQLESMLKRSLLAREEALGEAKLSDTEHKWLKEHRPKEARDWN
                     LLTNWRPETLRYVA"
     gene            1545..2045
                     /locus_tag="VFC15_03225"
     CDS             1545..2045
                     /locus_tag="VFC15_03225"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HZM09205.1"
                     /translation="MSEVDGLLRTSVELHCLGGFVLAVLYGLPRPTGDVDYIAAIPSN
                     TVTDLDSIAGQGSALARVYGLYFQHVTVADVPEGYADRLAELFPGRFTRLRLLALEVH
                     DLVLAKLVRNSPVDLEDAKFLAKTEKLDETVLRQRYEQELRPYLANESRHDLTLKLWL
                     DACFHS"
BASE COUNT          462 a          575 c          600 g          424 t
ORIGIN      
        1 acccacattc tgaacagttc tgggaggacc tgaacccatg gcattgctac atacaggcga
       61 tcgcatttgt gcgtcgtgcg gaggcatgaa cggcaaacac cggcccgggt gcattggcga
      121 cgacacagtt gaggtaatca gtacttatgg ccgccaacaa gcaatcgagg acggcatcct
      181 tgtcgattgc gagcaagctc caatgggcga aatgcggcgc cagcttatca agtggccgct
      241 tgcgatgact gctaccgcat ttcatcgcta cgtgtggcca atagatgaga aggcgaacct
      301 gccgccagga cagagtttgg aaggccgctt ctgggatgtg gtttggatgt tccatgccgc
      361 cgtcaagggg acagttccgg ccagacgcat cgatccgtgc aacctgctgt tcgacttcta
      421 ttgcatcgtt gctgatacgg cactgtggcc aaacgagagg attgacgcga ccgccaggtg
      481 tgggccagag gggatgcggc tggtaacgct gaaagccgtc tctggacctg gcgatgacgg
      541 cgaacccgtg atgacgttca tgttgccgga ggaagattga acgccgtgca tcgtgaggga
      601 agtggccgaa ttggcagcgc tgacaacaat caactcccca gatcagccct ggcgcggcag
      661 ttgccgggat gacaccacat gccaattcct cggtgctagc ctatagcgcc cacgccagcg
      721 actgatcaga cgtcacccct aacgtctgcg atcgcataaa tatcacacat ctgtgataat
      781 ctagagaaac atcccgaggc gaacactcat ggacgttgga catttcaaac gggcaagaaa
      841 gcaagccggc ctgactcaga agcgggctgc ggcgaaactc cgcgtgtccc agagctatct
      901 ttccatggtg gagaacggcc aaagggcatt gccgcccgaa ctcgcgagaa tgatggttca
      961 cgtttacaag ctttcgccgg tttcattccc gccgagtgaa acacgatggg acccaaagag
     1021 ggctgatccc gaccagcttg cggcggacct ggctggactc ggatatcccg gcttcgcgta
     1081 tctgcggaag cgacgttctg aaaggaatcc aggcgaactc ttgcttactg ccctggccca
     1141 ggaccgtttg gaggcgcggc tctttgaggc tctcccgtgg ctggtgctca agtactggga
     1201 gatggacaca ggatggctcg tccaggaggc caagctccac gatctgcaga atcgcctggg
     1261 atttgtcgtg tcgctagcca gaaccgctgg taacagggtg gcgctttcta atcctgaacg
     1321 agacgcagca ctcgaacaac tggaatccat gctgaagcgc agcctgcttg cgagagaaga
     1381 agctttgggc gaagcgaaac tcagcgacac agagcacaaa tggttgaaag agcatcgccc
     1441 gaaagaagct cgcgactgga atcttcttac caattggcgg cccgagacgc tcagatatgt
     1501 cgcttgatca cgcgcaagtc ctgccagtcc gctggaatgc attcttgagc gaagttgacg
     1561 ggcttctcag gacctccgtc gaacttcatt gccttggggg ctttgttcta gctgttctct
     1621 atggcttacc ccgtccaact ggcgacgtgg actacattgc ggcgatcccg tcgaacaccg
     1681 tcaccgacct ggacagcatc gctggccaag gttctgcgct ggccagagtg tacggactgt
     1741 atttccagca cgtcactgtc gccgatgtgc cggaaggtta cgcggaccgc ttggcggaat
     1801 tattccccgg ccgattcacc cgacttcgct tgctcgcact tgaggtgcac gacctcgttc
     1861 tcgccaagct tgtgcgaaac agtccagtcg acttggagga tgcaaagttt ctcgcgaaga
     1921 cagagaagtt ggatgaaacc gtgctcagac agcggtacga acaggaattg cgaccatatc
     1981 tcgccaatga gagccggcac gatctcacgc tgaagctgtg gctagacgcc tgtttccatt
     2041 cttagagcct tgttcgtccg g
//
LOCUS       DASRKK010000140         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_106685, whole genome shotgun sequence.
ACCESSION   DASRKK010000140 DASRKK010000000
VERSION     DASRKK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106685"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(599..2026)
                     /gene="ltrA"
                     /locus_tag="VFC15_03230"
     CDS             complement(599..2026)
                     /gene="ltrA"
                     /locus_tag="VFC15_03230"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM09206.1"
                     /translation="MTEGPNERSGKYDMDLRSDKQRNIQLELDFSSAPAGEAREAGRE
                     ETESLLTAHDTESPADTSRLMEEVCERENLKEALRRVKANKGSAGIDGMTVDEISDYL
                     KQHWPAIREQLLSGTYTPQPVRRMEIPKPDGEGVRKLGIPTVLDRFIQQAVTQVLQRR
                     WDRTFSERSYGFRPGRSAQQAVAQAQQYIAAGHDWCVDLDLEKFFDRVNHDKLMGQIA
                     KRVADKRLLKLIRAFLNAGVMENGLVSPSVEGTPQGGPLSPLLSNLVLDELDRELERR
                     GHGFVRYADDFNIYVRSERAGQRVMESVTRFIMQRLKLKVNETKSAVARPQERKFLGF
                     SFTAGPEVKRVIAPKALARFKRRVREITRRAKGVSMKTTIEELAPYMRGWRGYFGFCE
                     TPDVLLSLTRWVRLRLRAALWRQWKTPRRRREALLDLGVSSRLASNTASSGLGPWYLA
                     KTKALCIGLSNAYFKSLGLPSLIDE"
BASE COUNT          432 a          672 c          555 g          482 t
ORIGIN      
        1 ggctgatgga gcaatatgaa acgggaacgt tgaaaaagac gatcctcgaa gattggttgc
       61 gccagcacgt gcttcccgct tagaattctt ctcgctaaat aagaaacttt ctgcatgaag
      121 tgcgtgccgg gcgtcgaaag ccaaagaggt gatcgttgtc gtcgatacga acgtctagat
      181 ttctggaata ctcaacaaga acggtgtgcc acgtcgcgct ttagagcaag ccctggacgt
      241 tgatttaatc gctatttgcc atgaaatcga ggctgagata gttgatgttt tcgagcgcaa
      301 gttcggcatc tcaccggctg aaacgcttgt gcatctgcgg ctgtacctcg cgcacgcggt
      361 ctacgtggaa atcgacggga cagaacaggg ctgtcgcgat gccgaagacg acataatcct
      421 tagaatgcgc aaagaaaggc aaagccaacc tgattgtcac tggcgatcgc gacctcttgg
      481 aaatggtgtc cgtggaagaa atccgcatag atcgggatag ggggaagcct cgcggctccg
      541 cccctcccac accaccggac atgcgggtcc gcatccggcg gttcgagaag ttgggccatt
      601 actcgtcgat cagcgaagga agaccgagcg atttgaaata ggcgttagaa agccctatgc
      661 aaagggcctt ggtcttggcg agataccagg gaccaagtcc actgctggcg gtgttgcttg
      721 ccagtcgcga actcactcca aggtcaagca gcgcctcccg gcggcggcgc ggtgttttcc
      781 actgccgcca cagagcggcc ctgagccgca accgaaccca gcgagtgagc gacagcaaca
      841 cgtcaggcgt ttcgcagaat ccaaaatagc cgcgccaacc ccgcatgtac ggagccaact
      901 cctctatcgt tgttttcatg ctgacgccct tggcccgtcg cgtgatctcc cggactcgcc
      961 gcttgaaccg agccagagcc ttgggcgcga tgacgcgctt gacctccgga ccggcagtaa
     1021 agctgaaccc gagaaacttc cgttcctgcg gtcgcgccac cgcgctcttc gtctcattca
     1081 ccttgagctt gagcctttgc atgatgaatc gcgtgacgct ctccatcacc cgttgacccg
     1141 cccgttcgct gcgaacgtag atattaaagt cgtccgcata ccgaacaaag ccatgtcccc
     1201 ggcgctccaa ctcccggtcc agttcgtcga gcacgagatt gcttaacagt ggcgaaagcg
     1261 gtcctccttg tggagtcccc tctacgctcg gactaaccaa cccgttttcc atcaccccgg
     1321 cattcaggaa tgcccggatg agtttcagca gccgcttgtc cgcgactcgt ttggcgatct
     1381 ggcccatcag tttgtcgtga ttcactcgat cgaaaaattt ctccagatca agatccacac
     1441 accagtcgtg gcccgccgca atatactgct gcgcctgtgc cactgcctgc tgagccgacc
     1501 gccccggccg gaacccataa ctgcgttcgg aaaacgtccg atcccatctg cgttgcagaa
     1561 cctgcgtcac cgcctgctga atgaatctat ccagcaccgt cgggatgcca agctttcgca
     1621 ccccttctcc gtccggcttg gggatttcca tccgcctcac cggctgcggt gtataagttc
     1681 cgctcaacag ctgttcccgg atggctggcc aatgctgttt caggtagtcg ctgatttcat
     1741 cgacggtcat gccatcgatt cccgcgctcc ctttattcgc cttcacccgc cgcaatgctt
     1801 ctttcagatt ctctcgctca catacttcct ccatcaatcg actcgtgtcg gctgggcttt
     1861 cggtgtcatg ggccgtcaaa agcgattcag tctcttccct ccccgcttcg cgggcttcac
     1921 ccgctggcgc ggaagagaag tccagttcaa gctggatatt ccgctgcttg tcgctcctga
     1981 gatccatgtc gtacttgcca ctcctctcgt tcgggccttc agtcaccgtt cccggcttgg
     2041 cctatctgtt gactccgcct ttcggcattg gagtgcctca atagcctcgc cgacgacatg
     2101 acccactatg ccctctgctg acttctgccc cgcggtcagg t
//
LOCUS       DASRKK010000141         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_106750, whole genome shotgun sequence.
ACCESSION   DASRKK010000141 DASRKK010000000
VERSION     DASRKK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106750"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFC15_03235"
     CDS             <1..507
                     /locus_tag="VFC15_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM09207.1"
                     /translation="RSAAERLIARIWPLVKQRIPRAKLYIAGWNAAKYLEKYSSQPDL
                     TLANNLAHPSEFFSKAAVMVYAPSRGSGMKVKVMESMAYGVPVVTTWEGVEGIDYQNG
                     RDCWVAETDEDLAGKVCRLLESSVERQQMRDAARSLIEQRYSPRPVVDKMISVYQQLI
                     ASSKRPTL"
     gene            739..981
                     /locus_tag="VFC15_03240"
     CDS             739..981
                     /locus_tag="VFC15_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZM09208.1"
                     /translation="MEEVAISEFKAKCLALLERVRQTKKPLRVTRFGKPVADVVPATP
                     DAGQAAWIGSMKDTMQILGDIVSPVVDEADIEALRK"
     gene            978..1364
                     /locus_tag="VFC15_03245"
     CDS             978..1364
                     /locus_tag="VFC15_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM09209.1"
                     /translation="MKLLLDTHVWLWALQAPSRLNPRVRKELSNPANELWLSPVSTWE
                     ALLLNTRGRIRLPVNLAQWLAQATAPLREAPFTHEIVLAAQQLPLPHRDPADRFLAAT
                     AQVMDLTLVTSDDLLLGLGTIRTMKN"
     gene            1719..2009
                     /locus_tag="VFC15_03250"
     CDS             1719..2009
                     /locus_tag="VFC15_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZM09210.1"
                     /translation="MKTVNISDLKARLSAHIQFVKDGEEVLVCDRNEPVARIVPCRLA
                     NHSEQEQRLVARGALSPPLKKRRSASVSWPVPPGNVSDEVMQQVRQEEREGR"
     gene            2009..2440
                     /locus_tag="VFC15_03255"
     CDS             2009..2440
                     /locus_tag="VFC15_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM09211.1"
                     /translation="MRISAFWDTSALVPLCVRQGITPQAIALYKLHDLVIWWATPVEI
                     ASALARLVRMKQLDSGDWTRARKLAKRLADSWSVIQPSDALRAKSAQLVDRYDLRAAD
                     CFQLAAALEWCEDAPQGRVFLTADRRLRDVALLSGFDAKQM"
     gene            complement(2625..2795)
                     /locus_tag="VFC15_03260"
     CDS             complement(2625..2795)
                     /locus_tag="VFC15_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09212.1"
                     /translation="MEYIENQRGDLEPLIIFIPTGTVATTYCVIAESDVSTKLTGKGS
                     TEKARLGTAEAP"
     gene            complement(2767..3525)
                     /locus_tag="VFC15_03265"
     CDS             complement(2767..3525)
                     /locus_tag="VFC15_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09213.1"
                     /translation="MAKLEAGLDGREKVLAWLHVNQQRGGFQDLAIRRIETNGASVRL
                     PDMEDVESRFIYLCWRACNLRVLELKEAGLQKALLALCVGRFLGAERIPPDELFELQA
                     FREALKVFVLQWMLLDRVVEIISEEHFSGMRVLYDDTAAILKRDLEKAEILLAGFNVK
                     IAPRLGIEPITSSELDECLRADAPREADAITSLARATAELEFGNRLAGYPLAAKVLCG
                     YKSKAWDGFRQITLRDGGRGGREDDHGVHRKPAR"
     gene            complement(3541..3798)
                     /locus_tag="VFC15_03270"
     CDS             complement(3541..3798)
                     /locus_tag="VFC15_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09214.1"
                     /translation="MTALLGDTIKDLGMGLIDPKEATAVGYLAAGMLKALQQGDIENR
                     LRALEAVEGSKSTKSRSKVEHDDPPAPVPTIYSRLNAPKGN"
     gene            complement(4295..4759)
                     /locus_tag="VFC15_03275"
     CDS             complement(4295..4759)
                     /locus_tag="VFC15_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09215.1"
                     /translation="MNDENRGNVVLLSKRTAANRRNAQLSTGPRTEEGKNQSRRNALK
                     HGILASALLITGGEGAEDHAEFDRLLNSLYTDLDPVGTLEEMLVEKIAICYWRERRAL
                     RCEAGLIRENGSDYIILGGGLRVQSIVRYETAIHRQLVYAINQLGLQRARKG"
BASE COUNT          994 a         1436 c         1308 g         1141 t
ORIGIN      
        1 cgctccgcgg ccgagcgctt gatcgcgcgc atttggccgc tggtgaagca gcggattcct
       61 cgggcgaagc tttatatcgc gggctggaat gccgccaagt atttggaaaa atattcgtcg
      121 cagccagatc tcactttggc caataacctg gcgcatccgt ccgagttttt ttccaaagcc
      181 gcggtgatgg tctatgcccc ttcgcggggc agtggaatga aagtcaaggt catggaatcg
      241 atggcctacg gcgtaccggt ggtcactacc tgggaaggcg tcgagggcat cgattatcag
      301 aatggacggg actgttgggt tgccgaaacc gacgaagacc tggccggcaa ggtttgtcgc
      361 ctgttggaaa gctccgtcga acggcagcaa atgcgcgacg cggcgcggag cttgatcgag
      421 caacgctact cgccacgccc ggtggttgat aaaatgatct cggtctatca gcagctcatc
      481 gcttcttcca agcgccccac actctaaaag tgacaggtca gcctcgactc acctggtatg
      541 acgtcgagtc gggtgcggca ctcagacgcc gggccagttt aaaatgcgaa aatccaattg
      601 cacgagggat tcaagacaaa caacttattg ggttcgtttg gcaaaatgcc aattattcac
      661 accaatactt ttctcgtaac gcccttctat gaccatcatt taagaccatg atacactcat
      721 atagtcaaaa cgcagatcat ggaagaagtt gccatttccg aatttaaggc taaatgtctt
      781 gccctgctgg aacgtgtgcg acaaaccaag aaaccgctgc gcgtcacccg ctttggtaag
      841 cccgttgccg acgtcgtgcc ggctacgccc gatgctggtc aagcggcctg gatcggctcc
      901 atgaaggaca ccatgcagat tttgggcgac attgtgagtc cagtcgtaga cgaagcagac
      961 attgaggcac tccgcaagtg aaacttcttc tcgacactca tgtctggctt tgggccttac
     1021 aggcaccctc ccggctaaat ccccgagtca gaaaagaact cagcaacccc gctaatgagc
     1081 tgtggctttc tcccgtctcg acctgggagg cgctgttgct caacacaagg gggagaattc
     1141 gccttcccgt caatctggca cagtggctgg cccaagccac ggcgccgctg cgagaagcgc
     1201 ctttcaccca cgagatcgtg ctggccgccc agcaactgcc actgccgcac cgcgaccctg
     1261 ctgatcggtt tctcgctgct acggctcaag tcatggatct gacgctggtc accagcgacg
     1321 acctcttgtt gggcctcgga accatccgga ccatgaagaa ctgaaggcgc gagttcgagg
     1381 ttcgcgacgc gagaccgtaa tggttatgga ggagtacgac cgaggtgtcg atcgcactcc
     1441 cctccacgct ttattgatcc gttgagacct gaacgttcac tggcgccggt acggccgttg
     1501 catgaacttg gagccttcct ttttgtacgc cttgacgccc tccgacaaca acaactcgca
     1561 gatctgcaaa ggcttcgtgt gaagcggact ccccgtctct aaagctgtca gacgtgcgca
     1621 cacgaatcga ctccgaatct gccgaggtgg cagtagaagt tcaacggaaa caagaaatat
     1681 gagatgacta acatggtcat gttggctata attagcttat gaaaaccgtg aatatcagcg
     1741 atttaaaggc gcggctcagt gctcatatcc agttcgtaaa ggacggcgaa gaggtcctgg
     1801 tttgcgaccg taacgaacct gtggctcgta ttgtgccttg ccggctcgcg aatcattcgg
     1861 agcaggagca gcgactggtc gctcgtggcg ccctctcgcc accattgaag aagcgacggt
     1921 ccgcttcggt ctcatggccg gtgccgccgg ggaatgtttc cgatgaagta atgcagcagg
     1981 tgcggcagga agaacgggaa ggccgttaat gcgaatctcg gctttctggg atactagtgc
     2041 tctggttcct ctttgtgtac ggcaaggcat cacccctcag gccattgcct tatataagct
     2101 ccacgacctg gtgatctggt gggctacacc ggtcgagatt gccagcgccc ttgcccgact
     2161 tgtacggatg aagcaactcg actccggcga ctggactcgg gcccgtaagc tggccaaaag
     2221 gcttgctgat tcgtggtcgg tgattcagcc gtcggacgcc ttgcgcgcga agtcagccca
     2281 acttgtggat cgctacgatt tacgggcggc tgactgcttt cagttggcgg cggccttgga
     2341 gtggtgcgag gacgccccac agggccgcgt ttttctgacg gctgatcgac ggctaaggga
     2401 tgtcgccctg ctcagcggct tcgacgccaa gcagatgtga tcccaaaagc cactctcttt
     2461 tgaaactccg cagcctcctg ctccaaagtg cttccagtgt caacttctcc tccacattgc
     2521 tcttgccgac tgcgcctacg tcgtggccgc cctgtacggc tctactgatc gcctggtacc
     2581 tgaacgctta ctgatgccag gcgttctgac gcctgtgctg agattcaagg agcctctgca
     2641 gtgcccagtc gagctttttc tgtgctgcct ttcccagtga gtttcgtgct gacatcgctc
     2701 tccgcgatca cgcagtaagt ggttgcgacc gtgccggtgg ggatgaagat gatcaagggt
     2761 tcaagatcac cgcgctggtt ttcgatgtac tccatgatcg tcttctcgtc caccgcgccc
     2821 accgtcgcgc aaagtaatct gccggaaccc atcccaggcc ttcgacttgt agccgcacag
     2881 caccttggcg gccaaggggt aaccggcaag tctgttccca aattccaatt ccgccgtggc
     2941 gcgcgccagc gaggtgatgg cgtcggcctc cctcggtgcg tcggcccgca gacattcgtc
     3001 cagttcagac gatgtgatcg gctcaattcc gagtcgaggc gctattttca cgttgaagcc
     3061 ggccagcaat atctcagcct tttcaagatc ccttttcaga attgccgccg tgtcgtcgta
     3121 gagcactcgc atcccggaaa agtgttcctc gctgatgatc tccacaactc ggtcgagcaa
     3181 catccattgc aaaacgaaga ctttcaacgc ttcgcggaat gcttgcagtt cgaacagttc
     3241 atctggcgga atacgctcgg cgccaagaaa tcgacccaca cagagcgcca acaacgcctt
     3301 ttgtaggcca gcttccttta gttcgagcac tcgcaggttg caggcgcgcc aacacaagta
     3361 gatgaatctc gactcaacgt cttccatgtc gggcagtcgc acggaagcgc cgttcgtctc
     3421 aatgcggcga atcgcgagat cttggaatcc acctcgctgc tggttcacat gcagccaggc
     3481 gagaaccttc tccctcccat caagtcccgc ttcgagcttt gccaatctct tctcctgcat
     3541 ctagtttcct ttcggcgcat tcaaccgcga gtagatcgtt ggcaccggcg ccggtggatc
     3601 atcgtgctct accttggagc gcgatttcgt gctcttgctg ccctcgactg cttccagggc
     3661 gcgcaagcga ttctcaatgt cgccctgctg cagggccttg agcatccccg ccgccagata
     3721 tcccacagcg gttgcttctt tggggtcaat tagacccatt cccaggtcct tgatcgtgtc
     3781 gcccagtagt gcggtcacat cggcaatggt tctaagagaa cgctcagcgt accgggcgcc
     3841 cccgcacccc ttagttcctc cctcacggcg ccccacttcg gacactttct cggcacttgc
     3901 gtggaaataa caatgggtcc tgttttttag tgcccaagcc gtacaaggca caccgctttt
     3961 gtccgtgcat tgacatcgct tacccgtagc gatttgcggc tctgcggggg cagtcgcttc
     4021 gctgtaggaa ggagcagtgt tttgcgggcc ttctgggtct aattcctgac tcttttccct
     4081 catgggcgct ccttcgatct attccaaagc acactgcccc tggcggagca tgtcgatata
     4141 ttcgcggttt gccattccgc aaaactgtca gagatcaggg ttatgatcgg aacccttctg
     4201 ctagtcctgt tccatagcta atttaggaat cgcggagtcc cgtgcggctt tacggattgc
     4261 tcgagatcta aacggtcact ggcgccggta cgtgttaccc tttgcgtgcc cgttgtaaac
     4321 ccaactgatt tatggcgtaa accagctggc ggtgaatggc tgtttcatag cggacgatcg
     4381 attgaacgcg aagtccccca ccgaggataa tgtaatcact tccgttttcg cgaattagtc
     4441 cagcttcgca gcgcagagcc cgcctctccc tccagtagca gatcgctatt ttctcgacca
     4501 acatctcttc tagcgtgccg acggggtcaa ggtctgtata aaggctgttc aggagcctat
     4561 caaactcagc atgatcttct gcgccttctc ctccggtaat caggagtgcc gaggcaagga
     4621 tgccgtgctt aagggcgttg cggcgcgact gattcttccc ttcctcggtt cgaggcccag
     4681 tcgagagctg tgcgttgcgg cgattcgccg cggtcctctt actaagtaag acgacattgc
     4741 cgcggttctc atcattcaca tgccacgctc cgattcctat taaacagagt ttatcatgtt
     4801 cgcctttgtt tcgccagtga cttgggctcc agcagacgtc ttaaaccgta ctgttcagac
     4861 accggcggat cgtcggcga
//
LOCUS       DASRKK010000142         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_9323, whole genome shotgun sequence.
ACCESSION   DASRKK010000142 DASRKK010000000
VERSION     DASRKK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9323"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..986)
                     /locus_tag="VFC15_03280"
     CDS             complement(156..986)
                     /locus_tag="VFC15_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZM09216.1"
                     /translation="MPRKLDHTAALEHLLRADRRLARIIVRSGECRLQQETTQSIFEA
                     LLESIIYQQLNGKVAAIITARVKALFPENGRRLRTRRGLVTGFPSPEQILAAPEELLR
                     SAGLSRAKMLAIRDLAAKTLDGTVPTVKQAHRMSDEELIERLDTVRGVGRWTVEMLLL
                     FRLGRPDVLAIDDYGIRKGFAKMHKLAELPKPKELLAYGERWRPYRSVACWYLWRAAE
                     MKDLPMASDGAKKTAAPGAKKASVAKKTSIAKKASSGQSRKPAQARKKSASRLGKNSG
                     "
     gene            complement(1240..1806)
                     /locus_tag="VFC15_03285"
     CDS             complement(1240..1806)
                     /locus_tag="VFC15_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZM09217.1"
                     /translation="MPFKRSHKTYDGESLYEYAVGALARQMRTVAEIKRLMRNRVKEQ
                     EHGDLLVELVVARLKEQKYLNDTTYAENYSRFRKENEKFGRLRVIQDLKIKGVHGDII
                     DSVVSAAYGDVNEEQLAREFLQRKRMVKPVGQKQAARVFRAMMRAGFSSRVIFRILKK
                     WDVDDETLAALESETVEAQGPKEELDSG"
     gene            2269..3510
                     /locus_tag="VFC15_03290"
                     /pseudo
     CDS             2269..3510
                     /locus_tag="VFC15_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            3637..3990
                     /locus_tag="VFC15_03295"
     CDS             3637..3990
                     /locus_tag="VFC15_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09218.1"
                     /translation="MIVVSGFKVYPTEVESVIATLPGVTECAVVGVPDAVTGEAVKAF
                     IVKRDAEPSFEGVLSHCRQNLTSYKVPKFIEFCSDVPKNAIGKVLRRELRPTPIEPRG
                     ATTPEASVPIQELAR"
     gene            complement(4565..4981)
                     /locus_tag="VFC15_03300"
     CDS             complement(4565..4981)
                     /locus_tag="VFC15_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09219.1"
                     /translation="MMKFLVIAFMSLALALPAFGKTYKSTYPVACSELWGAVKDTLSI
                     PENYTVVESDDTQMTASYNVKHSAHVNVTGALLQRTNKVTLISKGAGCEMHVVSNYSG
                     WEHNDQGDFKTRVDESLAKLKAATPSQPAKPADAAK"
     gene            5348..>5941
                     /gene="guaA"
                     /locus_tag="VFC15_03305"
     CDS             5348..>5941
                     /gene="guaA"
                     /locus_tag="VFC15_03305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZM09220.1"
                     /translation="MVETQAIVVLDFGAQYSQLIARRIREQNVFSVVLPCTASLDEIK
                     SYSPAGIVLSGGPSSVYDADAPPADERVLHLGLPVLGICYGLQFMVYKLGGKVRAADK
                     REYGHAQVELLRSANDTPLFKGLQGPLSVWMSHGDEAMELPPGFELIGRTSNAVAAIQ
                     NPAQKYYAVQFHPEVHHTPQGTEILRNFVFEICGLTPN"
BASE COUNT         1194 a         1741 c         1717 g         1289 t
ORIGIN      
        1 aatcaacaca aagggcagga cgcacgccag ggtgagcccc gcttccaagg ccacacgaca
       61 gagataactg cgaagcggca cgccgcattc tactccttaa tcccctcaca aagctgtccg
      121 aaccagaaag ccggctttct cgaaaaccag gtcggctacc ccgagttttt ccccaacctg
      181 ctagcggatt tcttccgggc ttgggcgggc tttctggatt ggcccgacga agccttcttg
      241 gcgatcgaag ttttcttggc gaccgaagct ttcttggcac cgggcgcggc tgtcttcttt
      301 gctccatcgc tcgccatcgg gaggtccttc atctcggcgg cgcgccacag gtaccaacat
      361 gcaacgctgc gatacggccg ccagcgctcg ccgtaggcca gcagctcttt tggcttgggc
      421 agctcggcga gtttgtgcat cttggcgaag cccttgcgga tgccgtagtc gtcaattgcc
      481 agcacgtcag gccggccgag ccgaaacagc agcagcatct cgaccgtcca gcggccgacg
      541 ccgcgcaccg tgtcgaggcg ctcgattagt tcctcgtcgc tcatgcgatg ggcttgcttg
      601 acagtcggca ccgtgccgtc gagcgtcttg gccgcgaggt cgcggattgc cagcatcttg
      661 gcgcgcgaca gtcccgcgga acgaagcagt tcttccggcg ctgccagaat ctgttccggg
      721 ctgggaaaac cggtgaccag ccctcggcgg gtgcgaaggc gcctcccgtt ctcgggaaac
      781 agcgctttta cgcgcgcggt gatgattgcg gcgaccttgc cgttgagttg ctgatagatg
      841 atcgactcca gcagcgcctc gaagatgctc tgcgtggttt cctgctgcaa gcgacattcg
      901 ccggagcgaa cgatgatccg cgccagccgg cggtcggccc gcaaaagatg ttccagggcg
      961 gcggtgtgat cgagttttct tggcatgttc tttaattcta accgggaatt taggggcgga
     1021 attttcacca cggagaacac agaggacacg gagggagaag aaagttcaag tagtgggtca
     1081 gtttgaaata ttctttacat cgggcgcaac caacctggcc agtctcgtca tcgctgattc
     1141 ttgccaccgc ccacaatttc aaactgaccc actacagaaa ataagactac tccgtgctct
     1201 ccgtgacctc cgtggtaaag acagctcgca agagtcacgc tatccgctgt caagttcctc
     1261 tttaggccct tgtgcttcca cagtctcgct ttccagggcc gcaagcgtct cgtcgtcgac
     1321 gtcccatttc ttcaagatgc ggaagataac gcggctgctg aatccggcgc gcatcatggc
     1381 gcgaaacacg cgagccgcct gcttctggcc cacgggcttc accatgcgct tgcgttgcaa
     1441 aaactcgcgt gccagttgct cctcgttgac gtcgccgtag gcggcgctca ccacgctgtc
     1501 gatgatgtcg ccgtgtacgc ctttgatctt caggtcctgg atgacgcgca gccgcccgaa
     1561 cttttcgttc tccttgcgga agcgcgaata gttttcggcg taggtggtgt cgttgagata
     1621 tttctgttcc ttcaaccggg caaccaccag ttcgaccagc aggtcgccgt gctcctgttc
     1681 cttcacccgg ttgcgcatca ggcgcttgat ctcggccacc gtgcgcatct gccgcgccag
     1741 cgctcccacg gcgtattcgt agagcgactc cccgtcgtac gtcttatgcg agcgcttgaa
     1801 cggcatgacc agaagattat aggcggcaac gctgggagag aagcttcccg gaatccctct
     1861 tgcgatggcg gtctcttggt gctcgctcag ggggaccgct ggtgcaatgc cctgttctca
     1921 tcaagtgaca acccaagtag ctcgtatcgg ttggagcggg cacattcatg cacactggcc
     1981 gacttgtcgg gagtaatctc cccgaagccg gtttccggac aacctcagtg ttgcagacca
     2041 cagatccaca atcattggtc ggctttccgg aggtcttctt ttcttgacga caaatggcca
     2101 cttccattct gcgccagaaa ggtgtatgtg ttctgatatc aggagcaacg ctgccggagg
     2161 aatcggaact agagacgatt caaaggctgt ctaccgccct gtgatgggaa agccatgcgg
     2221 taaacccgcc ctctgagcag accggatggc tctgcaagga ggctatgaat ggacaagatc
     2281 tggctgaaaa gctatcccgc ccgcgtgccg cctgagattg acccaaatga attcgcgtcc
     2341 atcaatgaca tcctgcagag gacctttcgg cgtttcacac agcatgccgc ctaccgaaac
     2401 ttaggtacaa cacttaccta cgctcagatc gagcgcctga gccgcgattt tgccgcgtgg
     2461 ctgcaaggcg tgcccggact gcagaaggga tcgcgaatcg cgctcatgat gcccaacata
     2521 ctgcagtacc cgattgccct gttcggcgca ttgcgtgccg ggttgatcgt ggtcaacgtc
     2581 aatccgctgt acaccgcgcg cgaactggaa taccagctta gggactccgg cgcggaagcg
     2641 atcgtcattc ttgagaactt cgcccacgtc ctacagaagg tgctgcccaa cacgtcagtc
     2701 aagctcgtcg tcaccaccca gatgggggat attctggcga tgccacgtcg ctggttggta
     2761 aacgtcgccg tcaagcgctt caaaaggatg gtgcccgcct ggcatatcga gcgtgttgtc
     2821 agtttcagga ccgcactcaa gcgtggtgcc gaggcgcccc tgaatgagcc tatgttggaa
     2881 caccacgaca tcgcgttctt gcaatacacc ggcggcacca cgggtttgcc gaaaggtgca
     2941 gttttgacgc atgggagcgt gatcgccaat gtgttacaga tcagcgcgtg atcgagtggc
     3001 atcctcgaag aagggcagga aatttttgtt acgccgctgc ctttgtatca cgtgttctcg
     3061 ttgatagtga actgtttggt aggcgtgaaa gtcggagcgc tgaatctgct cgtcactaac
     3121 ccgcgcgaca tgcgtggttt tgtccgggag ttggagaact cgcacttcac catgatcacg
     3181 ggtgtcaata ctctttacaa tcgattgctg agcacgcctg gtttcgccga ggtcgacttc
     3241 agtggcgtga agttcgccgc cgtcggcggc gcccccgtgc agcgcgttgt ggcggaacgt
     3301 tggcaggcaa ctacgaagag ccccatggtt gaagcttatg gtctgacgga ggccacgctt
     3361 gccgtcacac tcaacatact cgactcggaa tacaacggta ccgcgggact ccccgtgccg
     3421 tccactgacg tgcgtatttc gggagaggag gactacgaac tgccgatcgg cgcggaaggt
     3481 gagatatgcg tgcgcgggcc ccagttgatg cagggatatt ggcagcaacc ggcggaaacc
     3541 gcgaaggcgt tcacggctga tggatggctc cgcacgggcg atattggcgt aatggacgag
     3601 cgcgggtatg tacgtattac cgagcgcaag aaggatatga tcgtcgtgtc gggtttcaag
     3661 gtctatccca cggaagtcga gagcgtaatt gcaactctgc cgggtgtaac agaatgcgcg
     3721 gtggtcggcg tgccggacgc cgtcaccggc gaggctgtca aggcgttcat tgtaaagaga
     3781 gacgccgagc cttcgtttga gggcgtctta tctcattgtc gccaaaacct gacgagttac
     3841 aaggtgccta agttcatcga attttgcagt gacgtaccaa agaatgcaat cggcaaggtc
     3901 ctacggcgcg aactgcgtcc gactccgatc gaaccgcgcg gtgcaacaac acccgaggcc
     3961 tccgttccaa tccaggagct ggcacgctga tgcagtctcg tcgaaggcgc cagttgtgtg
     4021 ggggccaaca aaaacttcaa tgactccgcc ttgcctgcag ggcgcggtgc gcagctttgg
     4081 agccggctgt tctgacgctg gctcgttgat gttttggtga cggctcgggc gattctgcag
     4141 gcttgtgtct cgcagacgcg aactgtccac tttgacagcg gcagtgacct gttcctatcg
     4201 aggctggagc cgtaacagca acaatctaaa cggcattggc gcgcctcttg tttcattccc
     4261 ccgtgggtcg gcttcgccgg tggaagactc acttgaagat aaatcgccat ctcacaaatg
     4321 agcgggccga agatgcccgt ttgcggaatt gtttgtgagt tttcacctca cgcgctaacg
     4381 ggcagagcac cgggcctgcg cgtgcggtcc tcgcatacgt gcattgccaa acgtcgctag
     4441 gcgatggcgt gaactactca gcaatgtccg gcaaaccgta ctcaacttgt gcaagcccgc
     4501 cttggggccg ccagcgcttt gcaaaactcc agccatgcgg gagtcagagg cgggcctggc
     4561 aaaatcactt tgctgcatcc gccggtttag ccggctggga aggtgtcgcg gctttcagct
     4621 tagccagaga ttcatccacg cgcgtcttga agtcaccctg gtcgttgtgc tcccatccgc
     4681 tatagtttga caccacgtgc atctcacagc ctgcaccctt cgatattaac gtgaccttat
     4741 tggttcgctg gaggagcgct ccggtaacgt tgacatgagc cgagtgcttc acattgtagg
     4801 aagcagtcat ttgggtgtcg tcactctcca cgacggtgta gttctccgga atgctgaggg
     4861 tgtctttgac cgcgccccac aactcgctgc aagcaacggg ataggtgctt ttgtaggtct
     4921 tgccgaacgc cggcagggca agagcaagcg acatgaaagc aataactaga aatttcatca
     4981 tggcaggtgc ctcctgataa aagatgtcct tcacagacgg catacgcctg gaccgcatct
     5041 cgacgcaatt gccagtcgat atccaaactg agcgccgtga ccacaccgga cagctaacgg
     5101 aaagtcacga cacgaagcct cggatagctc ttgctaaatc gccttatcgc cagcaatcgg
     5161 gctgtttagg agtcttgccc actgacagct tcccgcttcg ggaaccctga gtgctgacgg
     5221 ctgattgctg agtgctgatt gacgagggct gacggttgac tgctgactgc tgactgctga
     5281 ctgctaattg ccaattgctt cctgctgatc ccgcgcttta cactgctccc gtcctctggc
     5341 aaagtaaatg gtggaaacgc aagcgatcgt cgtcctcgac tttggcgcgc aatactcgca
     5401 actcatcgcg cgccgcatcc gcgagcagaa cgtcttttcc gtcgtcctgc cctgtaccgc
     5461 ttccctcgac gaaatcaaga gctattctcc ggcgggcatt gtgctgtcgg gcgggccgtc
     5521 gtctgtgtac gacgccgacg cgccaccagc ggacgagcgt gtcctgcatc tcggattgcc
     5581 ggtgctcggc atctgttacg gcttgcagtt catggtgtac aaactgggcg gcaaggtgcg
     5641 tgccgccgac aaacgcgagt acggccacgc ccaggtggaa ctgctgcgct cggcaaatga
     5701 cacgccgctg ttcaaggggc tacaagggcc gctgtccgtt tggatgtcgc acggcgacga
     5761 ggccatggag cttccgcccg gcttcgagtt gatcggccga acctcaaacg cggtcgcggc
     5821 cattcagaat cccgcgcaga aatattacgc ggtgcaattt catcccgaag tgcatcacac
     5881 gccgcagggc accgaaatcc tgcgcaactt cgtgttcgaa atctgcgggc tcacgccgaa
     5941 c
//
LOCUS       DASRKK010000143         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_9327, whole genome shotgun sequence.
ACCESSION   DASRKK010000143 DASRKK010000000
VERSION     DASRKK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9327"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFC15_03310"
     CDS             <1..375
                     /locus_tag="VFC15_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZM09221.1"
                     /translation="GTQSPIHTLDRGWGSCRDFAVLFVEAARSLSFGARIVSGYLYNP
                     NQNLVGSRDAGSTHAWAEVYVPGAGWITFDPTNRGVGGFNLIPVAVARDIRQAMPVAG
                     SFVGITHACMGMSVEVRVTSTV"
     gene            complement(1201..2160)
                     /locus_tag="VFC15_03315"
     CDS             complement(1201..2160)
                     /locus_tag="VFC15_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM09222.1"
                     /translation="MKQLTFSATGFERYGKTTRRAAFLSEMERVVPWADMCGLIEPFY
                     PKPGNGRPPYGLERMLRIYFLQQWFNLSDPGVEEALYDSAAMRDFAGIDLGHEAAPDE
                     TTICRFRHLLEAHDLGRRLFEEAHRHLEANGLKVSTGTIVDATIIHAPSSTKNASKTR
                     DPEMHQTRKGNQWYFGMKAHIGVDSKTKIIHSAAATAANVADCRLLPDLLHGEETRVW
                     GDQAYRGQRNVIVEHAPRAQDFTNRRYRQKGVVNENERAKNRTKSKVRAKVEHAFFVI
                     KQIFGFARVRYRGLDKNAHRLFVTCALANLYMARRHLLRARLT"
BASE COUNT          503 a          720 c          698 g          576 t
ORIGIN      
        1 ggcacccagt cgccgatcca cactctggat cgcggctggg gatcctgccg ggatttcgcg
       61 gtcctcttcg ttgaagctgc ccgcagtctt agtttcgggg cgaggatagt ctccggctat
      121 ctctacaatc caaatcagaa ccttgtaggg tccagagatg cgggatcaac ccatgcctgg
      181 gcggaagtct atgtgccggg cgcaggctgg attaccttcg atccaacgaa ccgcggcgtc
      241 ggcggcttca atctgattcc cgttgccgtc gcgcgcgaca tccggcaggc aatgcctgtg
      301 gctggcagtt tcgtcgggat tacccatgcc tgtatgggga tgtcagtgga ggtccgtgtt
      361 acatcgacgg tgtagcgccc cgcatcccgt gccacctcgg cttgtgtctt accatcatgc
      421 aacaccacaa tgacggccgt gcagagccac ttgtgcatct cccccaatgc tgcgaaagtc
      481 tctctgctca ccaaactatt atacgatggg ttccattggc tctcatgtat atacgtcgca
      541 gtgcagacgc catagccaga aggcttctaa ttgtctgaac aagagccatt tcttaaatga
      601 ctgacgttga ttccgatgag attgagtttc agtgcccaag ctgcggaaat gacctaaagc
      661 agacgatggc gcaattaaaa gtggagaggc acatggtctg tcctggttgt agcataggca
      721 taaatatcga cacggataga ttggccaatg ctgtcgaagc gatacaaagg gctaccgaaa
      781 aatctccccc tgagatcact attaaagttt tccgctagca ccaatggacg ccttgcatcg
      841 gtctcgtcgc cgacatctgc ttcaagatca tcggccatct tgcttctagt tcttcggctt
      901 ttcaacagac ggagccgatg ataggctcgc gggcattgcc gctaaagtga cgggaggtcg
      961 ccgtgctcac gctcgtaatt ggaatcctga ttatcgtcgt catcggtgcg attttgtttt
     1021 gggtcatcga taaattctgc cccgatgcgc ggctagggaa gctctgcgta agcggctgat
     1081 ttgatcgaac agcgactttc ttcggtgacg ggcgtagttg attgatgatt gtgacaggga
     1141 tccatgctgt ttttggcgat cgcccggcgg gcgcaggcgt tggcctgcgg gttggatgcg
     1201 ctatgtcagg cgcgcccgca gcaaatggcg ccgcgccatg tagagattgg cgagcgcgca
     1261 ggtcacaaac aatcgatgcg cgtttttgtc cagcccgcga tagcgaactc tggcaaaacc
     1321 aaaaatctgc ttgatcacga agaacgcatg ctcgaccttg gctctgactt tcgactttgt
     1381 ccggttcttt gcccgctcgt tctcattgac aacgcccttc tgcctgtaac ggcgattggt
     1441 gaagtcttgc gcgcgcggcg catgctcgac gatcacgttg cgctggccgc gataggcctg
     1501 atcgccccac acgcgggtct cctcgccgtg tagaagatcg ggcagaagac ggcagtcggc
     1561 gacgttcgcc gccgtcgccg cggccgaatg gatgatcttc gttttgctat cgacgccgat
     1621 atgcgccttc atgccgaaat accactggtt gcccttgcgg gtctgatgca tctcgggatc
     1681 gcgcgtcttg ctggcattct tggtcgagga tggcgcatgg atgatcgtgg cgtcgacaat
     1741 cgtgccggtc gagaccttca gcccgttcgc ctcaagatgc cgatgcgctt cctcgaacag
     1801 acggcggcca aggtcgtgcg cctcgagcaa atgccggaac cggcagatcg tggtctcatc
     1861 aggcgccgcc tcgtggccaa gatcgatccc tgcgaaatca cgcatcgccg ccgagtcgta
     1921 gagcgcttcc tcgacgccgg ggtcggaaag gttgaaccat tgctgcaaaa aataaatccg
     1981 cagcatccgc tcaaggccat aaggcggacg cccgttgccg ggcttcggat aaaacggctc
     2041 gatcagcccg cacatgtcgg cccacggcac gacccgctcc atctcggata aaaacgccgc
     2101 tcgccgcgtc gtcttgccgt aacgctcaaa acccgtcgcc gaaaatgtca gctgcttcat
     2161 cacaccagcc cgcctcgtct cgcccacgaa accttagcag cgatgcaagc cagtcggaat
     2221 cctgcttttg cagagattcc ttaagtttac agctcccgtc atggcgtggc tagaaagact
     2281 gctgacgcct cgattggcat ggatgttgaa acgcagacaa ctacgcatcg tcgggatcat
     2341 ggtagccttg aacgggctcc tgatcttcct gccaattcca ttcggtaata gcatcccagc
     2401 cttcgccgtg atggttatgg cgctcggcat gatgctcgga gacggtgtcg ctatactgtc
     2461 gggaattctt gtgtccatga tcgcgctctt gattgcc
//
LOCUS       DASRKK010000144         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_302196, whole genome shotgun sequence.
ACCESSION   DASRKK010000144 DASRKK010000000
VERSION     DASRKK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302196"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VFC15_03320"
     CDS             complement(<1..2223)
                     /locus_tag="VFC15_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09223.1"
                     /translation="MSIEPQRIGTPQTGFWTELRSGQAIMARQNGGGEFDLLLESGDR
                     VDRQEVRAWRAAAYSEADGRLTLTGTTRLALFATDLEVSVTYENVTAQVIKKTIRLYQ
                     NNIPRLFLRLRCSFEPPEAPETYWSFDHASHPGGPAYGVLADDVFPAAGYVLSGNRVF
                     GLLTASGWENGWARYGLRRSAAGNIPAVALVDPALLCTATAAERSAGKDYVALTLGEA
                     YENTLAPLESRGAGNGEYAFLGRREHPYTLILEVRQARTLAFEVRDPRGQVALTQTAA
                     FEEEAANAAEEGLHAFVLRLPALESSGEHKLRIASGEGPLELRRLQLFEVSPEPRPWH
                     ELRQGEALERTLFLFAEEMPPAPRSLRLRSQIHLADGLGFHGSAAEKVVFADAQMLTW
                     TSEPGLAEPMVVPSISYFEMYLRDAFWILNGLPDQFLNENILRRVGETINAEGNVGNI
                     ITAYHGSIEHKQNELAYLYLIWSLNNRLRFGSPPDLEKVRRVTGFVRRAFDPDGDGIV
                     LVNNPQSSADVVWQDRPSRFAVSQGYYALALRVARALGVEVGESEIESAEAAYRGYYA
                     DYGAEGAFLHAFPDNKLGEGGRPVGIISHVDLAPEFLSLYLFDHAMLTSEIVIATLEK
                     YPVSPQGLMPSFCRADGQFFTRDNNPFSGGLYWKPGTYWNGGSWLRLQYMALAVGKVH
                     GWVKAEHLLRQRLEAELTYDAENPVSREFLSCNGSPQDSSFHRVFGWNVFVLAVNEWL
                     N"
     gene            complement(2374..3264)
                     /locus_tag="VFC15_03325"
     CDS             complement(2374..3264)
                     /locus_tag="VFC15_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZM09224.1"
                     /translation="MTTQLTRPLEGALRGSGGASAGRRRRPLSLGAVVPYLPVAVIML
                     VYLFPLAFLLNTALKSNAEFFANPNGIAQTFEFGNFPTAWEKGNFGAYMLNSVLYTSV
                     AAFLGTVCSLLLGFPVSRGYLRHTRLWYGLFVAMLFLPNTLVTQFQLLLRLNLYDTQL
                     GYILIMAAGVGVGPLLVSGYVKSIPRELDEAAALDGVGYWRFVFLFLPHLIKPVLTTV
                     FLLQAIGVWNDIILPTILLPDQAKYPATLGLFAFQGTYTSEWSLLAAATIIVAGPLLV
                     AYVFLQRYLVASVVGAAVKG"
     gene            complement(3261..>3531)
                     /locus_tag="VFC15_03330"
     CDS             complement(3261..>3531)
                     /locus_tag="VFC15_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09225.1"
                     /translation="APSITANMLLCIVGSLQSYQLIYVLTGPNNPATQVLSLAIFTSG
                     FGGSSSGGAVQSQGYAAAISMVQFVMVTVISLTVLVYLRKREQVL"
BASE COUNT          713 a         1104 c         1099 g          615 t
ORIGIN      
        1 gttgagccat tcattgaccg ccagaacaaa gacattccag ccaaacacac ggtggaatga
       61 ggaatcctgg gggctgccgt tgcacgagag gaattcccgg ctcaccgggt tctccgcgtc
      121 gtacgttagc tcggcttcca ggcgctgccg gaggaggtgt tcagccttga cccaaccgtg
      181 taccttgccg accgccaagg ccatgtattg cagtctcagc cagctgcctc cgttccaata
      241 ggtccccggc ttccagtaca gcccgccgct gaaggggttg ttgtcccggg tgaaaaactg
      301 gccgtctgcc cggcagaaac tgggcatcag gccctgcggg ctgaccgggt atttctccaa
      361 cgtagcgatg acgatctctg acgtgagcat ggcatgatcg aacaggtaca ggctgagaaa
      421 ctccggggcc agatcgacgt ggctgataat gccaaccggc cttccgcctt cccccagctt
      481 gttatcgggg aaggcgtgca gaaaagctcc ttccgcgccg taatcggcat agtacccgcg
      541 gtaggcagcc tcagcggatt cgatctcgct ttccccgacc tccacgccca acgcgcgcgc
      601 tacgcgcagc gccagcgcgt agtatccctg gctgaccgcg aaccgactcg ggcggtcctg
      661 ccagaccaca tcggcgctgc tctgcgggtt gtttaccagc acaatgccgt cgccgtccgg
      721 gtcgaacgcg cggcggacaa agccggttac gcgccggacc ttctccaggt cgggcgggct
      781 gccaaagcgc agccggttgt tgagcgacca gatcaggtac aggtaggcca gctcattctg
      841 cttatgctcg atcgagccgt ggtaggcggt gatgatgttg cccacattgc cctccgcgtt
      901 gatcgtttct ccaacccggc ggaggatatt ctcgttcagg aactggtcgg gcagaccgtt
      961 caagatccag aaggcatcgc gcaggtacat ctcaaagtag gagatgctcg gcacgaccat
     1021 cggctcggcc agaccaggtt cgctggtcca agtcagcatt tgcgcgtcgg caaagacaac
     1081 cttctcagcg gcgctgccgt gaaaccccag cccatcggcc aggtgaatct ggctccgtaa
     1141 acgcaggctg cgcggggctg gcggcatctc ctcggcaaag agaaacagcg tccgttccag
     1201 cgcctcaccc tggcgtagct cgtgccaggg gcgcggctcg ggcgacactt cgaagagctg
     1261 caagcggcgc aactccagcg gcccctcccc ggaggcgata cgcagcttgt gttctccgct
     1321 gctttcgagc gccggcaggc gcaggacaaa ggcgtgcaag ccttcctcgg cggcgttggc
     1381 ggcttcttct tcgaacgcgg cggtctgggt aagggccacc tgcccgcgcg gatcgcgcac
     1441 ttcgaaggcg agggtgcgcg cctggcgcac ctcaagaatg agcgtgtagg gatgctcccg
     1501 cctgcccagg aacgcgtact ccccattccc cgcgccgcgc gactccagcg gcgcgagcgt
     1561 attctcgtag gcttcgccca acgtcagcgc aacataatcc ttcccggcgg accgttccgc
     1621 ggcggtcgcg gtgcaaagca gggctgggtc caccagcgcg acggcgggga tattgccggc
     1681 cgcggagcgc cgcaagccat agcgcgccca gccgttctcc caaccagaag cggtcagcag
     1741 cccgaacacc cggttcccgg acagcacata gcctgccgcc gggaatacat catcggccag
     1801 cacgccgtag gcgggtccgc ccgggtggct ggcatggtca aaggaccagt aggtttcggg
     1861 cgcttccggc ggctcgaagg aacagcgcag gcggagaaac agacgcggga tgttgttctg
     1921 atacaggcgg atggtcttct tgatcacctg ggcggtcacg ttttcgtagg tgacggatac
     1981 ctccaggtcg gtggcgaaca gcgccaggcg cgtcgtcccg gtcagggtca ggcggccatc
     2041 cgcttcggaa tacgccgcgg cccgccacgc gcgcacttcc tggcggtcga cccggtctcc
     2101 gctttccagc agcaaatcga actcgccgcc gccgttctgg cgggccatga tggcttgccc
     2161 cgagcgaagt tccgtccaga agccggtttg aggggttcca atacgctgcg gttcgatcga
     2221 catctttgtg aattccccgc caagcttgag tgctccagag ccaccacccg agaccgtctg
     2281 aggcacggaa gcattgccgc gcctcgtccc actccgaact cttcaacgag cacctcggtg
     2341 gcgtccgggt gccatcagtg agcggccaca tcgctaaccc ttgactgctg ccccaacaac
     2401 actggcaacg aggtagcgct gaaggaacac atatgcgacg agcagaggac cggccacgat
     2461 gatcgttgcc gcggccagca gactccactc gctcgtgtat gtgccttgaa aggcgaacaa
     2521 tccaagagtg gcggggtatt tcgcctgatc cgggagcaag atcgtcggga ggatgatgtc
     2581 gttccacaca ccgatggcct gcaacaggaa gaccgtcgtt agcaccggtt tgatcaggtg
     2641 cggaaggaac aggaagacaa agcgccagta gcccacgccg tccaacgcgg cggcctcgtc
     2701 aagctcccta ggaatggact tcacatagcc gctcacaagc aaggggccaa cgccgacccc
     2761 ggccgccatg atcaggatgt atcccagctg cgtgtcatac agattgagtc gaagcaggag
     2821 ctgaaactgt gtaaccaagg tgtttggcag aaacagcatg gcaacgaaca acccgtacca
     2881 caatcgcgtg tggcgcagat atcctcgcga aacgggaaat ccgagaagca gagaacagac
     2941 ggttccgagg aacgctgcca cagacgtata gagcacgctg ttgagcatgt acgccccgaa
     3001 gttccccttc tcccaggcgg tggggaagtt cccgaattcg aacgtctgag caatgccgtt
     3061 agggttggcg aagaactcag cattgctctt gagcgcggta ttgaggagaa aggctagggg
     3121 gaagagatag acgagcatga ttacggcgac gggtaggtac gggacaaccg cccccaggga
     3181 gagcgggcgt ctgcgtcggc cagcagatgc accgccgctc ccccgaaggg cgccttccaa
     3241 gggcctggtc agttgcgtgg tcatagcacc tgctccctct tgcgcaggta gacgaggacc
     3301 gtgagcgata tgactgtcac catcacgaac tgcaccatgg agattgcggc ggcgtatccc
     3361 tgagattgaa cggcgccccc ggaagagctg cctccgaagc cactcgtgaa gatggccaaa
     3421 gagagcacct gagtcgcggg gttgttcggg ccggtgagca cgtatatcaa ttggtagctc
     3481 tggagtgagc ccacaatgca gagcagcatg tttgccgtga tcgatggagc c
//
LOCUS       DASRKK010000145         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_400431, whole genome shotgun sequence.
ACCESSION   DASRKK010000145 DASRKK010000000
VERSION     DASRKK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400431"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            654..1259
                     /locus_tag="VFC15_03335"
     CDS             654..1259
                     /locus_tag="VFC15_03335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09226.1"
                     /translation="MLPEQTKAVAEHQRRRFNELVDVFDRPQPPEVMARLWEIVASAA
                     LRPGAVVLDVGTGAGVLIPLIQSYQPSMVLACDVAEKMLQRVHDKYPAVRTCHTDIVS
                     LALATTSVDAIFMNGMYGNIADKPAACSNAARMLRSGGRLVVSHPEGRAFVDQLRATS
                     DLFIESLPTKERFGIVLGPLGLEVIAYRDEPKLYLMVARKS"
     gene            1364..1711
                     /locus_tag="VFC15_03340"
     CDS             1364..1711
                     /locus_tag="VFC15_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZM09227.1"
                     /translation="MRFEGFSFGSIRIDGVSYYHDVVIDRGQIRERKKKPSKRFRDSF
                     GHTPLSLEEEIPWKCSRLVVGTGTGALPVMDEVRHEARRRKVELIILPTEEAMELLNR
                     RAGNTNAILHVTC"
     gene            complement(1808..2449)
                     /locus_tag="VFC15_03345"
     CDS             complement(1808..2449)
                     /locus_tag="VFC15_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZM09228.1"
                     /translation="MVTEHRLGDIIANSVTHGVGAALALAGAVVLVVTAVDGTAWQIV
                     SCSVFGCTLVLVYVCSTLYHSLVRTRARHVLRIIDHSAIYLLIAGTYTPFALVSLRGQ
                     LGWLLFAIVWSLAILGIIFKSFAIERFAIASVVVYLGMGWLAVFAMRPLQHALTWHGI
                     FWIALGGLFYTVGIVFYALDRISYFHAFWHLFVLAGSTCHYFAVLFYVAQPRT"
     gene            complement(2598..3227)
                     /locus_tag="VFC15_03350"
     CDS             complement(2598..3227)
                     /locus_tag="VFC15_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HZM09229.1"
                     /translation="MRKSALLLTLVVCIAVQAQDLKPIQLPTAQTDGGRPLMQVLKER
                     KTQREFSPEKLPLQVLANLLWAAFGINRPDGGRTAPSAMNWQEIDIYVALGDGLFLYN
                     AKANRLEPILAQDMRAATGTQAFVAAAPLNLVYVADLSKTGQSGSEAELFTAADVGFI
                     SENVYLFCTSEGLATVVRGSVDRAALAKIMKLGPNQKVLLAQTVGYPKK"
     gene            complement(3296..>4286)
                     /locus_tag="VFC15_03355"
     CDS             complement(3296..>4286)
                     /locus_tag="VFC15_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM09230.1"
                     /translation="EQMKESPFLFLRGTFYRWAQLWPALCAELCGAPKVLSVGDLHVN
                     SFGTWRDAEGRLCWGVDDFDESYPLPYTNDLVRLATSMKIVADVGELSVKPKEGCDAI
                     LEGYRKSLKEGGHAVVLEEHEHKLGKLGIDSFKPPTDFWGKLNRLPIVTRPLGSDVKR
                     ALEKTLPDPKMEYKLVRRQAGLGSLGQQRFVAIANWQGGCIAREAKAVLPSACTWLEG
                     KLGHGRSYYQQAISSAIRSHDPYQEIVGSWLIRRLSPDCNPIDIQDLPKRPDEQMLLH
                     AMGSESANVHLGTKSQVHNILKDLENRKSNWLRDAAKRMARAVEKEWKEYRKS"
BASE COUNT          979 a         1220 c         1216 g          871 t
ORIGIN      
        1 caccagttta gtcgatcatg ctgctcgcgc aaggggtttg ccagcggcgg ttccaccacg
       61 cttgctccat gcggggcctg tcaatccagc ggggccagca accgatgcgc agcgtccaag
      121 gcgctgctcc gtagcgatac agaactatag ttcctcaatc atttagcgtc aaacggcaac
      181 tatgggtgcg tgcccaaaaa gattggtgct gataaacacc gtgagtcccg aacgttaggt
      241 gaagctgtgg gtgcggaact caccaggctg cgcgtcgacc gaggctggag tcaactgaaa
      301 ctggcggatg cgctcgggta taccgagcgg tatataggcc agctggaacg gggaatcaag
      361 agcccgacgc tacgaacact cgccgatatc gccaaagcat tctcgataga gctttccacc
      421 ataatcaaag ctgcggagcg acgatatcgg catagcggaa gacactgacg ttcgcaagca
      481 gacacttgtg aggctgttgg tccgttcata cctcccttta tggaccccag agcctggttc
      541 cccgcacctc ggcttggtgc acgcgggctc ggcaggatcc gcgtaaaaca aacgaaccgg
      601 gggcggccca gcgcttcccg actgctggtt taggctaatc taggcaaaac gcaatgctcc
      661 ctgaacagac gaaggcagta gcggagcacc agcgtcgccg cttcaacgaa ctggtggatg
      721 tttttgatcg accacagcca ccagaggtga tggcacgcct ctgggagatt gttgcttcag
      781 cagcgctgcg tcccggagcg gtggttctgg atgtggggac gggagccggg gtcttgatcc
      841 cgttgattca gtcctaccag ccgtccatgg tgttggcttg cgatgtggcg gaaaagatgt
      901 tgcaacgtgt gcacgacaaa taccctgcag tccgcacctg ccacaccgac attgtctcgc
      961 tggccttggc cacgaccagc gtggacgcca tcttcatgaa tggcatgtac ggcaacattg
     1021 cagacaagcc ggctgcgtgc agcaatgcgg cacgcatgct tcggtcgggc ggacggcttg
     1081 tggtcagcca tcccgaaggc agggccttcg ttgatcaact gcgggccacc agcgacctgt
     1141 ttattgagtc gcttcccacc aaagagcggt ttgggatcgt cctcgggcca ctgggcttgg
     1201 aagtgatcgc gtatcgggat gaacccaagc tgtatctgat ggtggcgcga aagagctgac
     1261 gggtacgccg cttcgcatcg tatccgggcg catcgtggcg tattcaagca aattggcgcg
     1321 agactctggt tccacattga gcgtgctgca aggagatgcg accatgcgat tcgagggatt
     1381 ctcctttggc tccattcgga tcgatggggt cagctactac cacgacgtcg tgatcgaccg
     1441 cggacagatc cgcgagcgca agaagaaacc atcaaagagg ttccgggaca gtttcggtca
     1501 cacgccgctc tctttggaag aggagatacc gtggaagtgc agtcggttgg tggtgggcac
     1561 gggcactgga gcactgccag tcatggatga ggtcagacat gaggctcggc ggcgcaaagt
     1621 tgaattgatc atcctcccaa cggaagaagc catggagctc ttaaatcggc gcgccggaaa
     1681 caccaatgcc attctccatg tgacctgctg agcgttagat cccgccagac cggcgctggc
     1741 atgcggctag acgtcgaaga cctcatccga ggaaagctga gggcgagcag tgacgagcct
     1801 ttgcccgtca tgtgcgcggc tgggcaacat agaacagcac cgcaaagtag tgacaggtgc
     1861 ttccggccag cacaaagaga tgccagaacg catgaaagta agaaatgcgg tcgagagcgt
     1921 agaagactat gcccacggta tagaacaggc cacccagcgc aatccagaat atgccgtgcc
     1981 acgtgagagc gtgctgtaac gggcgcatcg cgaacacggc cagccaaccc attcccaggt
     2041 acacgacgac cgacgcgatg gcgaatcgtt ctattgcaaa actcttgaag atgattccca
     2101 ggatggcaag gctccataca atggcgaaca gaagccatcc caactgcccg cgcagcgaca
     2161 ctagggcgaa gggagtatag gttccggcga tcagtagata gattgccgaa tgatcaatga
     2221 ttcgcaggac gtgacgcgca cgagtgcgga cgagcgagtg atacagagta gagcagacat
     2281 agaccagcac cagcgtacag ccaaagaccg agcaactgac gatctgccag gcggttccat
     2341 ccaccgcagt gaccaccagt acaaccgctc ctgcaagagc gagcgccgcc ccgacgccat
     2401 gcgtgacgga gttcgcgatg atgtcgccca gcctgtgttc cgtcaccata ggaagatgat
     2461 aaacatcagg gcgttcagca ggcgcggcac tgcaacaacg gttcggcaaa aagattctcg
     2521 ccaagcgcca cgaagggcca agcacatgag atttgccgac tccacatccg acgaaagctg
     2581 ctccgcgcgg ggggacgcta cttcttcgga tagccaaccg tctgcgccag cagaaccttc
     2641 tggttgggtc ccaacttcat aatcttggcc agcgcggcgc ggtccacaga acctcggacc
     2701 accgtcgcca atccttctga tgtacagaat agatacacat tttcactgat gaaacctaca
     2761 tcggcggcag tgaagagctc tgcttcactt cccgattgtc cggtcttgct gagatctgcg
     2821 acgtaaacca ggttcaaagg tgcagcggcg acaaaggctt gtgttcccgt ggccgcgcgc
     2881 atatcttgcg ccaggatcgg ctcaagacgg ttcgctttgg cgttgtacag aaacaggcca
     2941 tcgcccagag ctacgtagat gtcaatttcc tgccagttca tcgcagacgg cgcggtacgc
     3001 ccgccatccg gacgattaat gccaaacgca gcccaaagga ggttggctaa cacctgcagg
     3061 ggcagcttct cgggactaaa ctcccgttga gttttccgtt ccttaagcac ctgcatgagc
     3121 ggacggcccc cgtccgtctg tgcggttgga agttggatcg gtttcagatc ctgggcttgg
     3181 acggcgatgc agacaaccag agtaagcagt aaagccgatt tgcgcataga gatcctcata
     3241 agccgtaaac ctgcgctggc acgaaatact gcggaaaagc tggctcggaa agccgtcaac
     3301 ttttgcggta ttctttccat tccttttcca ctgcgcgcgc cattcgtttt gctgcatctc
     3361 gcagccaatt cgattttctg ttttccaggt ctttcaaaat gttgtgaacc tgactcttgg
     3421 tgcctagatg gacattggca gattcagatc ccatcgcgtg tagaagcatt tgctcgtctg
     3481 gccgcttcgg caaatcttga atgtcgatgg gattacaatc cggcgaaagt cggcggatca
     3541 accaagatcc tacgatctcc tgatagggat catgggatcg tatcgccgaa gagatcgctt
     3601 gttggtaata cgaccgccca tgtcccagct tgccctccag ccatgtgcaa gcagagggca
     3661 ggactgcctt ggcttctcga gcaatgcagc ctccttgcca gttggcaatc gccacaaatc
     3721 tttgttggcc aaggctgcct aatccggctt gccgacgcac caacttgtat tccatctttg
     3781 ggtcgggcaa ggtcttctcc agtgcccgct tcacatcgct cccgagtggc cttgtgacaa
     3841 taggcaagcg gttcagcttt ccccaaaaat ccgtcggtgg cttaaaacta tcaatgccta
     3901 gcttcccgag cttgtgttcg tgttcttcca ggacgacggc gtggcctccc tccttgaggg
     3961 acttgcgata accttccagg atggcgtcac atccctcctt gggtttcaca ctgagctcgc
     4021 caacgtctgc aacgatcttc atgctggtgg ccaagcgcac aagatcgttg gtgtagggca
     4081 gtgggtagga ctcgtcgaaa tcatcgacac cccagcagag gcggccttcc gcatctcgcc
     4141 aagtcccaaa gctgtttaca tgcaggtcgc cgacggacaa aaccttcggt gcgccacaca
     4201 gttcggcaca aagtgccggc caaagctgag cccagcggta aaaggttccc cgcagaaaca
     4261 agaacggact ttccttcatt tgttca
//
LOCUS       DASRKK010000146         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1182091, whole genome shotgun sequence.
ACCESSION   DASRKK010000146 DASRKK010000000
VERSION     DASRKK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182091"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2652
                     /locus_tag="VFC15_03360"
     CDS             <1..2652
                     /locus_tag="VFC15_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM09231.1"
                     /translation="REARAASSLDHPNICAIYEFGEHEGRPFIVMQLLQGQTLRDCLA
                     SGALKDTASGAPVALDRILDIAIQIANGLEAAHEQGIVHRDIKPANIFITAKGVAKIL
                     DFGLAKLPLQPSAVQAIPAEIGGKQALNPSRPVRAAGTAAYMSPEQARGQPLDARTDL
                     FSFGLVLYEMATGRRAFTGDNAAALNDAILNRAPTPPAELNPELPTGLQDVIQKCLQK
                     DRNLRYQHAAEVRSDLEKVKRRREHPLLSRWRLLATAAVVVVTLLAGGLYWRSRRATT
                     LTGKDTIVLADFVNTTGEAVFDDSLKQALATDLEQSPFLTVLSDRKVTETLKLMNRPG
                     NERITKKVAEEVCLRNNSKVLLEGSIAPIGDHYLITLKAMNCQTGDTIASADAEAENR
                     NQIVKMLGKSSSQLRKKLGESRPSVEKFDTPLEEATTSSLEALEAFTEAQKMLTQGGS
                     AIPLLKRALELDPNFALAYASLGVVYHNLAETRSAIENFTKAYELRGRVSQRERLRIE
                     GYHYAYVTGQKEKAIETYSEWIRTYPEDPRPHAALSVIYAELGQYDKAAIEIQERIRL
                     SSEHSDYNLVGVYACLNRLDDAQAFLEQARQQDDSPSLRVTRYSLAFLRGNNAAMQEQ
                     LAWAMGKPGTEDILLSAQADTEAYYGRLAKARQLSQAAVQSAKHAEASERAAEWRANE
                     ALREAELGNADRARQMATEALVLSSGHDVEVNAALALASAGGAARAQKLVDKLNREFP
                     LDTVIQNYSLPSIRAAIELEKNDPAKAIDLLKAAVPYDLGSTSKSVVFYGYLYPVYIR
                     GQAYLKASQGQQAAAEFQKVLDHRGIVGNFVFGALAHLQLGRAQSMMGDKEAARKSYQ
                     DFFALWKDADPDIPILHSAKAEYAKLR"
BASE COUNT          662 a          853 c          878 g          569 t
ORIGIN      
        1 cgcgaggccc gcgccgcttc gtcgttggac catcctaata tttgcgccat ctacgaattc
       61 ggagagcacg aaggccggcc cttcatcgtg atgcagttgc tgcaagggca aaccttgagg
      121 gactgcctgg cctcaggcgc gttgaaggat acggcttcgg gagcgccggt ggccctcgac
      181 cggatactgg atatcgcgat tcaaattgcc aacgggctgg aggcggcgca tgaacaaggc
      241 atcgttcacc gtgacatcaa gcccgctaac atcttcatca ccgccaaggg cgttgccaaa
      301 attctggatt tcggtttggc gaagctgccc cttcagccga gcgcagtaca agcgatacca
      361 gccgaaatcg gtggcaagca agcattgaac cctagccgtc ccgttagggc cgctggtact
      421 gctgcgtaca tgtcaccgga acaagcgcgc gggcagccgt tggacgcgcg caccgaccta
      481 ttttcctttg gcctggtcct gtatgaaatg gcgaccgggc ggcgggcatt cacaggcgac
      541 aacgcggcgg cgttgaacga tgccatcctg aatcgtgcgc cgacacctcc cgcggagctt
      601 aatcccgaac tgcctactgg cctgcaagat gttattcaga agtgcctgca gaaggaccgc
      661 aaccttcgct accagcatgc tgcggaggtc cgctccgact tggagaaggt caagcgtcgc
      721 agggagcatc ccctgctcag ccggtggagg ctgctggcga cagctgcggt cgtggtcgtc
      781 accttgcttg ccggcggtct ctactggcgc tcgcggagag ccaccacgtt gacggggaaa
      841 gacacaatcg tgctggccga cttcgtcaac accaccggcg aagcggtgtt tgacgacagc
      901 ctgaagcagg cgctggccac cgacttggag cagtcaccgt ttctgaccgt gctctccgat
      961 cgaaaagtga ctgaaacgct gaagctgatg aaccgcccag ggaacgagcg gatcacaaaa
     1021 aaggtggcgg aggaggtctg cctgcgaaac aacagcaagg tgctgttgga aggctcgatc
     1081 gcccccattg gagatcacta cctgattacg ctgaaggcga tgaattgcca gacgggggac
     1141 acgatcgcga gtgcggacgc agaggcggag aaccgcaacc aaattgtgaa gatgctcggt
     1201 aaatcgagca gccagctgcg caagaagctg ggcgagtcgc gaccctcggt cgaaaaattc
     1261 gacaccccgc tggaagaagc tacaacctcg tcgctggaag ccctcgaggc ttttaccgaa
     1321 gctcagaaga tgctgacgca gggtggcagc gctattccat tgcttaagcg cgcgctggag
     1381 ctggacccaa acttcgctct cgcgtacgct tctctaggag tggtttacca caatctcgct
     1441 gagaccagat cggcgattga gaacttcact aaggcgtacg agttgcgagg ccgggtgagc
     1501 cagcgggagc ggttgcgaat cgaggggtat cattacgcgt atgtgaccgg ccagaaagaa
     1561 aaagctattg agacctattc ggagtggatc cggacctacc ctgaggatcc tagaccgcac
     1621 gccgctctaa gtgtcatcta cgccgagcta ggccaatacg acaaagctgc gatcgaaata
     1681 caggaacgaa ttcggttgtc atctgagcat tccgattaca acctcgtagg tgtctacgct
     1741 tgcctgaatc ggctggatga cgcccaagct ttcctcgaac aagcgcgcca gcaggacgac
     1801 agtccatcct tgcgggtgac aaggtactct ttggcttttc ttcgaggtaa caatgccgcc
     1861 atgcaggagc agttggcgtg ggccatgggc aagccgggca ctgaggacat tctgctatca
     1921 gcccaggccg atactgaggc ttactacggc cgtctcgcca aggcacgcca gctttcacag
     1981 gcagcggtgc agtcggcaaa gcacgccgag gcttcggaaa gggcggcgga atggcgagcg
     2041 aatgaagctt tgcgggaagc cgagcttggc aatgcagatc gggcgcgcca gatggcgact
     2101 gaggcgctgg tgctgagcag cgggcatgat gtggaggtaa acgctgcact tgcgctggca
     2161 agcgcaggag gcgcagcgcg ggcgcaaaag ctggtcgaca agctcaaccg tgagttccct
     2221 ctcgacaccg tgatccagaa ctactccctc ccaagcatcc gggcggccat cgaactggaa
     2281 aagaatgacc cggccaaggc gatcgacctg ttgaaggctg ctgtaccgta tgacttaggc
     2341 agcacatcta aaagcgtcgt gttctatggg tatttgtacc ccgtatacat tcgtgggcag
     2401 gcgtacctca aagcgagcca agggcagcag gctgcagcgg aattccaaaa agtgctcgac
     2461 caccggggaa ttgtaggaaa tttcgtcttt ggtgctcttg cgcacttgca gctcgggcgg
     2521 gcccagtcga tgatgggcga caaggaagcc gcccgcaagt cgtatcagga tttctttgct
     2581 ctctggaaag atgctgaccc cgacatcccc atcctccatt ctgccaaagc ggagtatgca
     2641 aagctaaggt agcctacatc ttgctactaa tttccgccga cttttcatca gtctgttcca
     2701 accgcctccc gccgttccct tacaatattc ctcatgcctg acgaacttct gcgctaccgc
     2761 tccgagttcc ccatcctcag ccgcactacc taatctcatt agtaactcat tgggtgctat
     2821 gccccgcact gtcgaagacg ctctacatca gtacacagac atgtggaaca cgcgcggcgt
     2881 tcgcgcctgg gaagaagaat ggtggatgct ggccgcgcag gtcggcgatg agatcggcgc
     2941 gctgttcaac gctcctcagg gc
//
LOCUS       DASRKK010000147         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_693639, whole genome shotgun sequence.
ACCESSION   DASRKK010000147 DASRKK010000000
VERSION     DASRKK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693639"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2287
                     /locus_tag="VFC15_03365"
     CDS             <1..2287
                     /locus_tag="VFC15_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09232.1"
                     /translation="LGQQDYTACVDVDHLPKIARRLNRAATMTACLVAADPSFHPPVS
                     GARVLLQRADGFRLFTGYLATAPEQRYLGYSQAGAAWRYTLQALDDSCLLDHNALPVR
                     TPFAFRTAGDAVRTLANDVLPNGLDLSGVQDVSPVNQFLIVPQKTWTEHVQELATMTR
                     ASYRAHDGKLYFEEVGQQSFTISEQDQHFVPEGLVLLQSDQLRNDVTIIGEFEPLVYV
                     RDYFLGDGTTLGFYLSDTPFSKTAVTIFEEDYTATSLEPTLWSVTDPNGRVSVSGGQL
                     QISGGPATVTFVEQLELAGGLMMQHGQVVFNAASSGTFGALYNGSVGDANCIAGFSIS
                     PNGANCSIQALINGAWVGSPLATVPGHLYSFTSQLFCNEAHRIHQTYLSSTHPAGNGR
                     GGDSVSAAMRVVLAVHDVDPNNPGTLVAPATVLYDDVLPAPPGFATYALANGPNLCVS
                     VSFTRLQHVVDTEVRSLIPGGQFRTRLTGNFADGGECYVTSSAELRFYPPYPPQINEQ
                     VVVAYRSSARAMARVQDQNSIAAHHNGSDSGRRSYVRRLKLPLAPTSIDCENAAVALL
                     DDTVQPAWAGEYRVISDFLPVGDVIPTNAVQISAPSRGAAFMAIVREVDLQVISLRDD
                     RSEYLLHFSNDAAELLAFKFETMILPEPLTAVFTTTVPSSSLYLPALTAAQVTNVIAT
                     EITVDAGIVPPPGGGIEVRRSDGGWGTGSDGNLAGRFTTQTFNLPRLSRVQGYYLRQY
                     DASSPAKYSRDSALLYVDYPL"
     gene            2719..3252
                     /locus_tag="VFC15_03370"
     CDS             2719..3252
                     /locus_tag="VFC15_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09233.1"
                     /translation="MKSFLYLLTLATAISIPALTQEPATTLQSSPPPGNTPVEQQKNA
                     ASATKDNSGISGDQVTKPAGAKGTTLIGCLSGPNPEGKYVLTSMQHRTGVQVLGPDDL
                     KNDSGSKAKLTGSWETSPQLQPQAKQKEARRFQVAAVEVLSQHCTPPSEKTPVSKEKQ
                     RQQQQKSSPGDQMSPKQ"
     gene            complement(3483..3932)
                     /gene="dtd"
                     /locus_tag="VFC15_03375"
     CDS             complement(3483..3932)
                     /gene="dtd"
                     /locus_tag="VFC15_03375"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZM09234.1"
                     /translation="MRAVVQRASRATVKVGDEITGEIGKGLLVLLAVGQEDSEADADY
                     LAEKVVGLRIFEDEAGKMNLSVGEIGGAILAVSQFTLFGDVRRGKRPSFDAAARPERA
                     RELYASFVERIRALGLRCETGRFQEMMEVELVNSGPVTILLDSKKVF"
BASE COUNT          815 a         1363 c         1104 g          851 t
ORIGIN      
        1 tttgggacaa caggattaca ccgcttgcgt ggacgtcgac catctgccga agatcgcgcg
       61 gcgcttgaac cgggcggcca ccatgacggc ctgtctcgtc gccgccgatc ccagctttca
      121 tccgcccgta agcggagcgc gcgtgctctt gcaacgcgcc gatggatttc gtttgttcac
      181 tggctacttg gctacggcgc cagaacagcg atatcttggc tacagccagg cgggcgccgc
      241 ctggcgctac actttgcaag cgctggacga ttcctgcctg ctagatcaca acgcgttgcc
      301 ggtcagaacg cccttcgcct ttcgcaccgc gggcgacgcc gtgcgcacgc tcgccaatga
      361 cgttctgccc aacgggctcg atctctcggg agtgcaggac gtcagtccgg tcaaccagtt
      421 tcttatcgtt ccgcagaaaa cctggaccga gcatgtacag gaactcgcca ccatgacgcg
      481 cgccagctat cgcgcccatg acggcaaact ctattttgaa gaggttgggc agcagagctt
      541 tactatcagc gagcaggatc aacactttgt tcccgaaggg ttggtactgc tgcaatcgga
      601 ccagttgcgg aacgatgtga cgatcatcgg cgagttcgaa ccgctcgttt acgttcgtga
      661 ctacttcctt ggcgatggca ccacgttggg cttctatctt tcggacactc cgttcagcaa
      721 gactgcggtc acaatcttcg aggaggatta cactgcgacc tcgctggaac ccacgttgtg
      781 gagcgtcacc gatcccaacg gcagggtctc agttagcgga ggtcaactgc aaatcagcgg
      841 tggccccgcg accgtcacct tcgtcgaaca attggaactg gccggcggcc tcatgatgca
      901 gcacgggcag gtcgtgttca acgccgcgtc cagcggtacc ttcggcgctc tctacaacgg
      961 ctcggtgggc gatgccaact gcatcgccgg cttttcgatt tcgcctaatg gcgccaactg
     1021 ctcgattcag gcactcatca acggcgcttg ggtcggctct ccgctggcga ccgttcccgg
     1081 gcatctgtac tctttcacca gccagttgtt ttgtaacgaa gcccaccgca ttcatcagac
     1141 atatttgtcc tcgactcatc ccgcggggaa cggtcgaggc ggcgactctg tatccgccgc
     1201 gatgcgggtc gtgctcgcgg tgcacgatgt tgatcccaat aatcctggaa cgctagttgc
     1261 gcccgcaacc gtgctctacg acgacgtgct gcccgcgcct ccgggcttcg ccacctacgc
     1321 gctggcgaac ggcccgaact tgtgcgtcag cgtgtccttc acccgtttgc agcacgtcgt
     1381 cgataccgaa gtgcgcagtc tgattccggg cgggcagttc cgcacccggc tgacaggcaa
     1441 ctttgccgat ggcggcgagt gttacgtgac ctcgtccgcc gagctgcgat tctatccgcc
     1501 gtatccgccg cagatcaatg agcaggtcgt ggtcgcctat cgcagtagcg ctcgcgccat
     1561 ggcacgggtg caggaccaga acagcatcgc tgcgcatcac aacgggagcg acagcggacg
     1621 ccgcagctac gttcggcgat tgaagctgcc actggctccc acctcgatcg actgtgaaaa
     1681 cgcggcggtg gctttgctcg acgacaccgt gcagcctgcc tgggccggcg aataccgcgt
     1741 catcagcgac ttcctccccg tcggcgacgt tatccccact aatgcggtcc aaatttcggc
     1801 gccgtcgcgc ggcgcagcat tcatggccat cgttcgtgag gtggatctgc aagtgatctc
     1861 ccttcgcgac gatcgctcgg agtacctgct gcatttctcg aatgacgccg ccgagctgct
     1921 cgccttcaag tttgaaacca tgatcttgcc cgagccgctc acggcagtgt tcaccaccac
     1981 agttccgtcg tcgtccttat atcttccggc tctcaccgcg gcccaagtca ccaacgtcat
     2041 cgcgaccgaa atcacggttg acgcgggcat cgttccgcct cccggcggcg gcatcgaagt
     2101 gcgtcgctct gacggcggtt ggggcaccgg gagcgacggc aaccttgccg ggcgcttcac
     2161 cacccagacc ttcaatctgc cacgcctctc gcgcgtgcag ggctattacc tgcggcaata
     2221 cgatgcctcg tcgcccgcca aatactcgcg cgattcagct ctgctgtatg tggactatcc
     2281 gctgtgagca gcctgccccg gagcgacgat agcggatgca gtttgggtaa agcggacttt
     2341 tatgaatccg ccgttggtgc ggcaaatgcc atctcctgca agaacatcgt tgatgccaac
     2401 aagttccagg aagagcgcaa agtcatcgac ggcgtagttg cctgtctaaa ggcgtctgtg
     2461 tgggcccaca aggagaaccg ttacccgtgg cgcccctggt tgccgtgtcg tcgcggagtc
     2521 gtcatctcca cagagccacc gaggcaggga gaatttctga ttcaatatcg acaaaagaag
     2581 gccttctgcc ttaattctca aatgattttt ctctgtgcct ctgtgtctct gtggtgatcc
     2641 ccggtctccg ttgcaacttc ccctcgctac gccgcgtcga accctcaggt atcctcgata
     2701 ctcacctgag gtttgcaaat gaagtcgttc ctctatctcc tgacgctggc aaccgccatt
     2761 agcatccccg cgttgaccca agagcctgcc accaccctgc agagttctcc tcctcccggc
     2821 aacacccctg tcgaacagca gaagaacgca gccagcgcga ccaaagacaa cagcggcatc
     2881 agcggcgacc aggtaaccaa gccggccggc gccaagggta ccactctcat cggttgtcta
     2941 agcggaccca acccagaagg caagtatgtc ttgaccagca tgcagcaccg tactggagtt
     3001 caggtgcttg gtcccgatga cttgaagaac gattccggaa gcaaggccaa actgacaggc
     3061 tcgtgggaaa ccagccctca gctgcaaccg caagcgaagc aaaaggaagc gcgccgcttt
     3121 caggtggccg cggtcgaagt gctctcgcaa cactgcacac cccccagcga gaaaaccccg
     3181 gtcagtaagg aaaaacagcg gcagcaacag cagaagagca gccccggcga tcaaatgagt
     3241 ccgaaacagt gacgggccgt agatccgcga gcgtcaacca gctgcggaaa aactcgattg
     3301 catttcgctt tgaaggggcg caccttcagg tgcgccgtta cgagtccttt attttgtcat
     3361 cccgaaccga ctttagtcgg tgagggatct gcttttcgac tttttccgca gcctgtgaag
     3421 ccgtgcccct tcaaatcaga ttcgcgcgcg gagttttctc cgcagcctga caatctacaa
     3481 cctcaaaaca ccttcttcga atccagcaga atcgtgactg gcccgctgtt caccaactca
     3541 acttccatca tctcctgaaa gcgtccggtc tcgcagcgca accccagcgc acgaatacgc
     3601 tccacgaagc tcgcgtacaa ttcgcgcgcg cgctccggcc gcgcggccgc atcgaacgat
     3661 ggccgtttgc cgcgcctgac atctccaaac agtgtgaatt gcgaaaccgc cagtatcgcc
     3721 ccgcctatct cccccacgct gaggttcatc ttccctgcct catcctcaaa gatccgtaat
     3781 cccaccactt tctccgcaag gtagtcggca tcggcctcgc tgtcctcctg cccaactgcc
     3841 agcaacacca gcagaccttt tccgatctcc ccagtaatct cgtctcccac cttgaccgtc
     3901 gcccggctcg ctctctgcac cacagcccgc atggcagcgt tgtatcacag ccccgtagcg
     3961 caaggacacc gcgagccggc ccgttcctgc gccgaaaaga atttacccct cgcgttgacc
     4021 ttgccaagcc caccgtttag gatgaatcgg gcatcatcta tctgagcgct ctcgaagcta
     4081 atcgtgtgtt tcggctgtct gaagggcctc ggcattcttt acacggcttc ctg
//
LOCUS       DASRKK010000148         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_106918, whole genome shotgun sequence.
ACCESSION   DASRKK010000148 DASRKK010000000
VERSION     DASRKK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106918"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFC15_03380"
     CDS             <1..522
                     /locus_tag="VFC15_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09235.1"
                     /translation="IEVRGFRGVLNSTTNVVLEGMEAGMTFEESVRQAQKLGVAESDP
                     TDDIEGVDAAVKVIALANVLMNAGLKLDDVSRQGIGGITAEQLREARASGEAWKLVCG
                     ARRAEDGRVVASVAPRRLKLSDPLAQVKGTSSVISFETDVFPELILTEQDPGLEATAY
                     GLLADFLTAVRSS"
     gene            524..1798
                     /locus_tag="VFC15_03385"
     CDS             524..1798
                     /locus_tag="VFC15_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZM09236.1"
                     /translation="MSSSARTAVSARKLDVAWVRQQFPSLYQAVNGQPAAFFDAPGGT
                     QVPQRVIDAIANYLSTSNANAGGTFLTSQRTDRMIADARAAMADFFNCASDEVVFGAN
                     MTTLTFALSRALGRELKSGDELLVSCLDHDANVSPWVCLEERGVKVRTADIKPGDCTL
                     DMFDLSAKIRRNTRMVAVGWAANAVGTINDVHEIVRLAHAAGSMAFLDAVHYAPHGPI
                     DVRALDCDFLACSSYKFFGPHLGILFGKREQLQRLRPYKLRACTEKLPDRWETGTQNH
                     ECLAGLIAAIDYIADVGRHHDPEVKTRREAILAAYEVFQRHERELVEQLINGLLAIPG
                     LTFYGIKDAAKFDQRTPTVAIRMEGYTPRELATHLGESGIFTWDGNYYAVDLAERLGV
                     QQSGGMLRIGLAHYNTAEEVERLLTELRGLAK"
BASE COUNT          391 a          559 c          656 g          415 t
ORIGIN      
        1 atcgaggttc gtgggtttcg tggggtgttg aactccacga ccaacgtggt cctggaaggt
       61 atggaagcgg ggatgacgtt cgaggaatcg gtccgacagg cgcaaaagtt gggagtagcg
      121 gagtccgatc cgaccgacga catcgaaggc gtggatgccg cagtgaaagt aatcgcgttg
      181 gccaatgtcc tgatgaacgc cggcctgaaa ctggacgatg tgagccggca gggtatcggc
      241 ggaataacgg cggagcagtt gcgcgaagcg cgcgccagcg gcgaagcttg gaagttggtt
      301 tgcggtgcca ggcgtgcgga ggatgggcgc gtggtggcgt cggtcgctcc ccggcggctc
      361 aagctttctg atccgctggc acaggtaaaa ggaacttcgt cggtcatcag ttttgaaacc
      421 gacgtttttc cggaattgat ccttaccgaa caggaccccg gtctggaggc gacagcgtat
      481 ggtttgttgg cagacttcct aaccgcggtc aggagttcat agtatgtctt cgagtgcaag
      541 aaccgctgta tcggcacgca agctggatgt ggcctgggtg cgtcaacagt ttccttcgct
      601 gtatcaggcg gtgaacgggc agcctgccgc gttcttcgat gccccgggcg gtacccaggt
      661 gccgcaacgc gtgattgatg cgattgccaa ttatctgtcc acgagcaatg cgaacgccgg
      721 cgggaccttc ctcaccagtc agcgcaccga tcggatgatc gccgatgcgc gcgccgcgat
      781 ggcggacttc ttcaattgcg cgagcgacga ggtggtcttc ggcgccaaca tgaccacgct
      841 gacctttgcc ttgagccgcg ccctcggtcg agaactgaag agcggcgacg agttgctggt
      901 gagctgtctg gaccatgacg cgaatgtctc cccttgggtg tgcctggaag agcgcggggt
      961 gaaggtgcgc acggctgata tcaagccggg cgattgtacc cttgacatgt tcgacctctc
     1021 ggcgaaaatt cgccgcaaca cccgcatggt agctgtcggg tgggctgcca acgccgtggg
     1081 cacaatcaac gacgttcacg agattgtgcg gctggcgcat gcggcaggct ccatggcctt
     1141 cctcgatgcg gtacattacg cgccccatgg gccgattgac gtccgcgctc tcgattgcga
     1201 cttcctggca tgctccagct acaagttctt cggtccccac ctcggcattc ttttcggcaa
     1261 gcgcgagcag ctgcagcggt tgcgtcctta caaacttcgc gcctgcaccg agaagttgcc
     1321 tgaccgctgg gagacgggaa cgcagaacca cgaatgcctg gccggtttga ttgcagccat
     1381 cgactacatc gccgacgtgg gtcgtcacca cgatcctgag gtgaagacgc ggcgcgaggc
     1441 catcctcgcg gcgtatgagg ttttccaacg gcacgagcgc gaactggtcg agcaattaat
     1501 caatggattg ctggcgattc cgggactcac gttctacggc atcaaggatg cggcgaagtt
     1561 cgaccagcgc accccgacag tggccatccg catggagggt tacacgccgc gcgaactcgc
     1621 gacccacctt ggcgaaagcg gtattttcac ttgggacgga aactactacg ctgtcgatct
     1681 cgcagagcgg ctgggggtgc agcagagcgg aggtatgttg cgcatcggct tagcgcacta
     1741 caacacggcc gaagaggtgg agcggctgct gacggagttg cgtggattgg cgaagtagca
     1801 actagccttt agcctttagc atttagcatt tggtaccaag cggaggcgat ttggtcgttg
     1861 catcgaagtc aggccctcgg cggcccttca cttggattat tcagaacggc aatgaatcct
     1921 aatggtgtca tcctgagcga agcgctcgcc tgacgtctcc gacccgcttc gtttgcgggt
     1981 cgggcgagcg cgcagtcgaa ggatctgcgg tttttcccgg a
//
LOCUS       DASRKK010000149         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_498031, whole genome shotgun sequence.
ACCESSION   DASRKK010000149 DASRKK010000000
VERSION     DASRKK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498031"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1118..2059
                     /locus_tag="VFC15_03390"
     CDS             1118..2059
                     /locus_tag="VFC15_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016721870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM09237.1"
                     /translation="MANGFERYTKKTRRAAFLEEMEQVVPWSELCSLVEPHYPKAGNG
                     RRPVGIERMLRIYFLQQWFNLSDPSVEEALYDSPLMRHFAQIDLGNEPVPDETTVCKF
                     RHLLEEHKLGGAMLETVNLHLESRGIKIATGTIVDATIIHAPSSTKNAGRQRDPEMHQ
                     TKKGNQWYFGMKTHVGVDSKMKIIHSVVVTAANIADSAALPDLLHGDETKVWGDQSYR
                     GQSAAIHDIAPRAQDLTHKRYRHKDRVDEVEREKNRTKSHIRSRVEHVFAVIKLKFGY
                     TKVRYRGIKKNANQVFTLCALSNLFLLRRRLLVTQGA"
     gene            2509..>2793
                     /locus_tag="VFC15_03395"
     CDS             2509..>2793
                     /locus_tag="VFC15_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZM09238.1"
                     /translation="MRIWMNVSSRKGCLLHSIYLAEDKAISYSEVLQHPPMRELSSRR
                     RFLRKAAFAGGGALLGTVSLNQISPRIWREPLVFEPNRSYWARSQPRQNLS"
BASE COUNT          683 a          765 c          773 g          572 t
ORIGIN      
        1 gcattaagcg atcatacggc gtgcgcaagt tcacctgccc tggacgtacc cggctcccga
       61 agcaactctc tggtatgcgg cgagtgctca tcgacgactt ccgggcacga agtgcacgct
      121 tatgccggta tttgcatcga caacctgtgc tggatggtgc ggatcgagat gaaaaccgac
      181 cgtggacgct atcacaacga ggaatccgag agcagcatca attggtgccg caacgcaagc
      241 ttagccattc ggatgtcacc gctgggatcg caaagacaat attgtgccgc gcactcgtca
      301 cattgcacaa tttcacaacg ttcattcggt tgcaccgcag cgcaacgttc atcggaatgt
      361 atccgaaagg cgttagtcga ccagtcatgc gttgatctct tctagctagg acttctcgtg
      421 catcaccctc gaagcgaata gtctggctca acggagagcg gcttcttctt tgccgcgtca
      481 gggcagacgt gagcgttttc cacgatagtc accacgtcga ttttggtcgc cgctccgagc
      541 cgagagaaac agagcagaca taccgtttcg aataatccgg gtcgaagctc gagtcgagca
      601 aatccttcag gcgaggacaa tatactggag aagtggggag tgctatttcc tgcgaacatg
      661 agcctacctt cacaatgggc tgctgctaac ggacacaata tccagctact ccggttgaat
      721 acaacgataa tttcgcaacg gaaggaataa gttcccgtta tggcgagagc ccggctgtgg
      781 tggcttcgcc ggaagaacta aatggttggg ttggactgga ggtcgcagaa cccgtccaca
      841 tcgcaaccaa agcgaccgat ttgagcgccg aattgaagcg gggcctcccg tttgctagga
      901 aacagaggcg agatcaaacg agaaagtccg actaaccagt actggaaact caactcttct
      961 catgggtgtc cgaattggca tgtggagata cggcgcagac ccgtaacgaa acaatcgcac
     1021 ttactaggga gactctgatt aagttctctg ctttccacag ccagtcaaga taaagtgcag
     1081 ccagatgaaa cctcggacca aacagaggac actggcgatg gcgaatggat tcgagcggta
     1141 tacgaagaag acgcggcggg ctgcgtttct ggaagaaatg gagcaggtgg ttccgtggag
     1201 cgagttgtgt tcgctggtgg aaccgcatta tccgaaggcc ggcaacggac gccgtccggt
     1261 gggcatcgag cgcatgctac ggatttactt tttgcagcag tggttcaatc tgtcggaccc
     1321 ctccgtggaa gaagcgctgt acgactctcc gctgatgcgg cacttcgcgc agatcgatct
     1381 tggcaacgaa ccggtgccgg acgagacgac ggtctgcaag ttccgccact tgttagaaga
     1441 gcacaagctc ggcggcgcga tgctggagac agtgaacctt cacctcgaaa gtcgcggcat
     1501 caagatcgcc accggcacca tcgtcgatgc gacgatcatt cacgcgccca gttctacgaa
     1561 gaatgcgggc cggcagcgcg atcccgagat gcaccagacc aagaagggca accagtggta
     1621 cttcgggatg aagacgcacg tgggcgtgga tagcaagatg aagatcattc actcggtggt
     1681 ggtgacggca gccaacatcg cggattcagc agcgctgccg gatttgttgc acggcgatga
     1741 aaccaaggtc tggggcgatc agtcctatcg cggacagagc gcagcgatcc acgacattgc
     1801 gccacgagca caggacctga cccataaacg ctaccgtcac aaggaccggg tggacgaagt
     1861 cgaacgtgag aagaatcgaa cgaagtcgca cattcgctcc cgcgtcgagc acgtgtttgc
     1921 agtgatcaaa cttaagttcg gctacacgaa ggtgagatac cggggaatca agaagaatgc
     1981 caatcaggtg ttcacgctct gtgcgttgag caatctgttt ctcttgcgac gcagattgct
     2041 ggtcacccaa ggggcgtagt gtctctggac actctccaga ggtgacgaca atctccacgt
     2101 agtcggaaac atcgctctaa aatgccgaag tccctctccc gctaacataa aagaactgct
     2161 tcgccaccac ttatgcagac cttccctaga gagctaccac tgccgtcggc ccgagcgcaa
     2221 atgcgcaaga ttctctctga tcggcgcttt ggcagtgcgg tttcgagacg agtctgcacc
     2281 ggagaaattc ggtgactcgc cgctagcgaa tgatcgatgt gcgaaccgat gatgaaccta
     2341 tctcaccgga cagccgcttc agaagcattc acgacgggca caatttcgat cgtggcccga
     2401 cgatcacagt atttgaaatc gataagccga cttcccatta ctaattgcag taagtcagcc
     2461 tcagatcact tccggttagc cgacgactcg cccagaatcc ccgaatccat gcgcatctgg
     2521 atgaacgttt catcccgcaa gggctgtcta ctacactcca tttaccttgc cgaggataag
     2581 gcgatctctt attctgaggt tttgcagcat ccgcccatgc gagagctctc aagccgacgc
     2641 cgcttcttaa gaaaggcggc cttcgcgggt ggcggtgcgt tactcggcac cgtcagcctg
     2701 aaccagatct ctcccaggat ctggcgtgaa ccgctggtgt ttgagcccaa ccgcagttat
     2761 tgggcaagaa gtcaaccgcg tcagaatctg tct
//
LOCUS       DASRKK010000150         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1377526, whole genome shotgun sequence.
ACCESSION   DASRKK010000150 DASRKK010000000
VERSION     DASRKK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377526"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFC15_03400"
     CDS             <1..953
                     /locus_tag="VFC15_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HZM09239.1"
                     /translation="YGFRPGRSPHQALDALTVGIQRKRVNWVLDADIRGFFDNLSHAW
                     TMRFVEHRVADRRILRLIRKWLKAGVSEDGQWSETKLGTPQGAVASPLLANVYLHYLF
                     DLWADVWRRKVAKGDVIVVRYADDLVVGFQHRTDAERFLKEFRERLAKFGLELHPDKT
                     RLIEFGRFAARDRKARGEGKPETFTFLGFTHYCGQRHKSGTFTVWRITAKKRMVAKLK
                     AIKAELQRRMHHRMAAVGAWLRKVVLGYYQYHAVPGNTTQLRIFKLRVCRLWQSVLVR
                     RSQRAKMCWERFTPILNRWIPPPRVLHPYPDARFYATHPS"
     gene            1187..1960
                     /gene="rlmB"
                     /locus_tag="VFC15_03405"
     CDS             1187..1960
                     /gene="rlmB"
                     /locus_tag="VFC15_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZM09240.1"
                     /translation="MVVIYGIHSVSEALKSGARSFEYVGIARERHDQRVQRLIDDCRA
                     SGVQVRFLPREQLDRVANTNTHHGVIAVTSSKQYADVDTLLDQRHGEHSFLVVLDSVE
                     DPHNLGALIRTADAAGADGVIIPERRAAGLTGTAVKASAGASEHLPIARVTNISRTLD
                     ELKERNVWMVGLDERGTQNYDEVDYNMHCAIVLGAEGKGLHDLVRRRCDFLVSIPMLG
                     HVPSLNVSVAGGVVMYELVRQRRKTERAGKREPMRAQND"
     gene            1953..2255
                     /locus_tag="VFC15_03410"
     CDS             1953..2255
                     /locus_tag="VFC15_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09241.1"
                     /translation="MTNRVVVAVTAGQAPPAAMFVTAVVAAGLAICGWLCIYKTDALV
                     EQQRRRYEKHGWIRAYPFSGMVMKSWYPTYLRCSGIFVWLWDLVLIYLLWVRKPVR"
     gene            2231..>3764
                     /locus_tag="VFC15_03415"
     CDS             2231..>3764
                     /locus_tag="VFC15_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09242.1"
                     /translation="MGTKAGAVKGRLVTVVLLVGAAVHAVALDREAFTISRYQLEVQV
                     DRSAHVIAVTGKLTLRNDSNKPQKLATLQVSSSLAWNAIALDDKPVQWLGDNYTSDID
                     HTGSLSEAIVTLPKEVAPAATVTVDVQYGGTITPDATRLTRMGAPADVAARNDWDEIS
                     DPFTAVRGLGYVAWYPVAIEAVSLSDGNAVFDAIARWKQRHDRSEFDAAFAIGAGNGK
                     QPLRIVTNMADSGEGNLAPEVRDDKTSAPLVTKTSVLGLGETVPTFAVADYVELARPT
                     VTLLHVADHTSLARDYALAAEANDPLLHDWLGEPTRPARIVELTDPNANPWQNGAVLF
                     TPLREAPTATLQLLLLPAQVAARFHSPHAWIQDGLERFLQTLAVERRSGRHAALEFLD
                     QYREPLVQVEEAAHPKATKSSPDSNAASNTLLNTNDELYLRGKGGFVFWMLRDMTGND
                     AMQRAIASFRGGADKDPSYFQHLLQSSAKRDLEWFFDDWVYRDRGLPEFHVESAYTRP
                     LLS"
BASE COUNT          754 a         1127 c         1161 g          722 t
ORIGIN      
        1 cttatgggtt ccgccccgga cgcagcccgc accaggcgct ggacgcgctg acggtgggca
       61 tccaacggaa gcgggtgaac tgggtgctcg atgccgacat ccgtggcttt ttcgataacc
      121 tgagccacgc atggacgatg aggttcgtcg agcatcgggt ggccgatcgc cggatacttc
      181 gcctgatccg gaaatggctg aaggcgggag tatcggaaga cggccagtgg tcggagacaa
      241 agctgggcac accgcaggga gcagtggctt cgcccctgct cgccaacgtg tacctgcatt
      301 acctcttcga tctatgggct gatgtctggc gcaggaaagt ggctaaaggc gacgtaatcg
      361 tcgtccgcta tgccgacgat cttgtggtgg gctttcagca ccgaacagat gccgaacgtt
      421 ttctgaaaga gttccgggaa cggctggcga agttcggctt ggaattacat ccggacaaga
      481 cacgactgat cgagttcggg cggttcgccg ctcgcgaccg gaaagcgcgg ggagagggaa
      541 aaccagagac cttcacgttc ttgggtttca cccactactg tgggcaacgc cacaagagcg
      601 gaaccttcac cgtatggcgg atcactgcga agaagcgaat ggtcgcgaag ctcaaagcca
      661 tcaaggctga gcttcaacgc cggatgcatc atcgcatggc cgcagtcggt gcgtggctcc
      721 ggaaggtcgt gctgggctat taccaatacc acgctgttcc cggaaacacg actcagttgc
      781 gcatcttcaa acttcgcgtg tgcaggctat ggcagagtgt tctggtccgc cgcagtcagc
      841 gcgcaaagat gtgttgggaa cgttttaccc caatcttgaa ccggtggatt cctccacccc
      901 gcgttctgca cccctatccc gatgcacgct tctacgccac tcatccttcg taagagccgt
      961 atgcgtagac gcgctcgtac ggatctgtgc ggggggcgat cagcaatggt tgtccctacc
     1021 gcgacacaac tcacatgcgg catcctcaaa gaggatagcg gttcagtcga aagcaggtcc
     1081 ctccgctcgc ccgcagcggc gggctcgatc gggatggcaa accgtaaacc accgagagct
     1141 gacagctgac ggccaacggc tcccttgtcg tacactaatt tcaaacatgg tcgtcatcta
     1201 cggtatccat tcggttagcg aggcgctaaa gtcgggggcg cgcagctttg aatacgtcgg
     1261 cattgcgcgc gagcggcatg atcagcgggt acagcgcctg attgacgact gccgcgccag
     1321 cggtgtgcag gtgcgcttcc tgccgcgcga acaactcgat cgcgtcgcca acaccaacac
     1381 ccatcacggc gttatcgctg ttaccagcag caagcaatac gcggacgtgg acaccctgct
     1441 cgaccagcgc cacggtgagc atagtttcct ggtggtgctc gacagcgtgg aagatccgca
     1501 caatctgggc gcgctgatcc gcaccgccga tgcagccggc gcggacggtg tcatcatccc
     1561 ggagcgccgc gccgccggat tgacgggcac tgcggtcaag gcttcggcgg gagcgtccga
     1621 gcatctgccg attgcgcggg tgaccaacat ctcgcgcacc ctagatgagt tgaaagagcg
     1681 caacgtgtgg atggttggcc tcgacgaacg cggcacccag aactacgacg aagtggatta
     1741 caacatgcac tgcgcaatcg tgctgggcgc cgaaggcaaa ggtctgcacg atttggtccg
     1801 caggcgctgc gactttctgg tctcgattcc catgctcggc cacgtgccat cgctgaatgt
     1861 gtcggtggcg ggcggggtgg tgatgtatga actggtccgc cagcgccgga aaaccgaaag
     1921 ggccgggaag cgcgagccga tgcgagctca aaatgactaa ccgagtggtt gtcgccgtaa
     1981 cggctggaca ggcaccgcct gccgcgatgt ttgtgacggc tgtcgtggcg gcaggcttgg
     2041 cgatttgcgg ctggctgtgc atctacaaaa cagacgcact cgtcgaacaa caacgcaggc
     2101 gctacgagaa gcatgggtgg attcgggctt acccattctc cggcatggtg atgaagtcct
     2161 ggtatccaac ttacctgcgc tgttcaggaa tatttgtgtg gttgtgggat ttggtactca
     2221 tctatcttct gtgggtacga aagccggtgc ggtgaagggg cggctcgtta ccgtggtgtt
     2281 gctcgttgga gcggccgtgc atgcagttgc cctcgaccgc gaggccttca ccatctcgcg
     2341 atatcagctt gaagttcaag tggaccgcag cgcgcatgtc atcgcggtta ccggcaagct
     2401 cactctgcgc aacgattcca acaagccgca gaagctggct actttgcagg tatcgtcgtc
     2461 gctggcatgg aatgccatcg ctctggatga caagcctgtt cagtggctcg gcgacaacta
     2521 cacctccgac atcgatcaca cgggaagcct ttcggaggca atcgtcacgt tgccgaaaga
     2581 ggttgctccg gcggccacag tcactgtcga cgtgcaatac ggtgggacca ttacgccgga
     2641 tgcgacgcgt ttaacgcgca tgggcgctcc cgccgacgtg gcggcgcgca acgattggga
     2701 cgaaatcagc gatcccttca ccgcggtgcg cggactcggc tatgtcgcct ggtatccggt
     2761 ggcaatcgaa gcggtgagcc tgagcgacgg caatgccgtc ttcgacgcca tcgcgcgctg
     2821 gaagcagcgg cacgaccgca gcgagttcga cgctgcgttc gcgatcggag ccggaaatgg
     2881 caagcagcct ttgcgaatcg tgactaacat ggctgactct ggcgaaggca acctggcacc
     2941 ggaagtgcgc gacgacaaga cttccgcccc gctggtgacg aagacaagtg tgcttgggct
     3001 gggagagacc gtcccgacct ttgcggtcgc cgattacgtc gagctggcca ggcctactgt
     3061 cacgcttttg catgttgccg atcacacttc gctggcccgc gattacgccc tggccgccga
     3121 ggccaacgat cccctgctgc acgactggct gggcgagccg actcggccgg cgcgaatcgt
     3181 cgaactgaca gatcccaacg ccaatccctg gcaaaacggc gcggtgctgt tcacgccgct
     3241 gcgcgaggcg ccgaccgcga ccttgcagct tctgctgctc ccggctcagg tggcggcgcg
     3301 gttccattct ccacacgcct ggattcagga tgggctggag cgcttcctgc aaaccctcgc
     3361 ggtcgaacgc cgcagtggac gacatgcagc cttggaattt ctcgaccaat atcgcgagcc
     3421 cctggtgcaa gtggaagaag cagcgcatcc gaaagccacc aagtcctcgc cagacagcaa
     3481 tgccgccagc aacactctcc tcaacaccaa tgacgaactc tacctgcgtg gcaagggtgg
     3541 cttcgtgttc tggatgctgc gcgacatgac cggcaatgac gccatgcaac gcgccattgc
     3601 ttcctttcgc ggcggcgccg ataaagaccc gagctacttt cagcatctgc tgcaaagcag
     3661 cgccaaacgc gatctggaat ggttcttcga cgactgggta tatcgcgatc gcggcttgcc
     3721 ggagtttcac gtggaatcgg cgtacactcg ccccctgctt agcg
//
LOCUS       DASRKK010000151         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_9579, whole genome shotgun sequence.
ACCESSION   DASRKK010000151 DASRKK010000000
VERSION     DASRKK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9579"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..928
                     /locus_tag="VFC15_03420"
     CDS             278..928
                     /locus_tag="VFC15_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09243.1"
                     /translation="MDSADGHRRARDDARQAIALDPNLSDGYLALGHYQIDYEWNWAA
                     GEQSLKQAARLQPGSADVIGLRAFLFRTMGNLNEAISLQEQLIALDPLNVGSYRLLGF
                     SFYAAGRYEDALAAARKALEINPQTGKAHSQAAEILLAQGKLQQALAETEQESLAWAR
                     LTDEAVVYHALGRQQDSDAALAKLIARHHEQGAFQIALVYSYRKELDKAFEWLDRA"
     gene            complement(1323..4016)
                     /locus_tag="VFC15_03425"
     CDS             complement(1323..4016)
                     /locus_tag="VFC15_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZM09244.1"
                     /translation="MRPYRIRTFILVVVSLAFIGWAIPTAANFIIEYNWWKEVAQVDT
                     WISMLWYSIAPAAIVSLLAFIAFYVAHARGLHFAGIRQRDYPVYSRLVPVGLALVAIW
                     FASASIDFWTVMRFFGSRGITAPSDAWQDHVFSHSLPFYLFDLPFYSDLLGFLFGLAI
                     LSALLFWGTARGWQLSERIRYRQLMDGPGRPINFGAYLRLPGATRNSFVRVLAVILLL
                     GFAAWVYLGNFELLLNTHAFMTGADFVDEKVTLPLRWLLIVSALVALPLVWLSKFKQA
                     AVLVVSCFALQLALPAVVRAVYVRPNEISIERPYIERHIEATTAAFGLNRNATESPFT
                     PSGQGVVDPVQDATLLANVRLWDLRAYGATITQIQALRPYYTFPDTDVDRYFIGGKIK
                     QLLLSPREIDVTQLSAEASESWINPRFIYTHGIGTVVAEVNKTTPDGLPVLLIENAPP
                     EIKSPGFKLTRPEIYFGEKTQDPVFVHTAREEFDYPSGDQNKYSTYQGTGGFPVGSFP
                     LKLAAAISQGEPNIVFTGYLTGQSRMMIYRRVQARLAHLAGFLHWDPDPYMVITDDGR
                     LVWMVDAYTTSLSHPYSAALPVAGIEEGANYIRNSVKATVDAYTGEMSLYVFDPSDPI
                     IQAYQKLFPKLFRPASEMPADLRRHARYPEALFRTQAEAYRVFHMRDPQVFYNKEDIW
                     EIARDLFGQSGQPEPLAPTYVVATLPGEKQPEYLLILPFSPRGKDNLIGWMAARCDGD
                     QLGKLIFYQLPKQQLMYGPMQIESRIDQDQNISKDLTLWNQQGSHVLRGSIIALPVTG
                     GFLYLESIYIQATEARMPQLKKIVLAMGDRLIYRDTFDEALADLTGAAAPPVVAAAQT
                     TAAASSEKNATTLAERIHQLRDQAEQMARELEKLEKEAVRK"
     gene            complement(4578..>5096)
                     /locus_tag="VFC15_03430"
     CDS             complement(4578..>5096)
                     /locus_tag="VFC15_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09245.1"
                     /translation="SAGLMKTTPPILRIYTQDDLAKLQRQNADAIERMTKSLNQEAHF
                     LAYAPPTFIGFRQGAYLQLSITTQLNAAAGTSRYKLAALAFDDHISHLVRPALNYFPG
                     DPGFDGINFTSMIHVTDGSSPLAVEFFFPFRIMRCFASYDCTGQQLIDSGTVVINGER
                     SALDLQIAEGKN"
BASE COUNT         1201 a         1377 c         1490 g         1028 t
ORIGIN      
        1 caggctcatc cttcgattag aaaagactgt ctgggcatcc ggttcagacg ggcccgatcg
       61 cagccggaac ctcacgcgat tctgccgacg cccgaattaa catggcaacc tgcttgcgat
      121 tacagtggcc agagaacccc caagcatgac gctttgagtt ttggcgaggc acaaggcact
      181 cacttacgct gacaaagcca tacaactgga cccacgcttt gcggccgctt gggcactgcg
      241 atcgatggtg atgcaggagg gagtcgacgg cggattcgtg gacagcgcgg acgggcatcg
      301 tcgggcccgc gacgatgcgc gccaggcgat cgcacttgat cccaacctct cggacggtta
      361 tctggcgtta gggcactacc agatagacta cgagtggaac tgggccgctg gcgagcaatc
      421 cctgaaacaa gctgcacggt tgcagcccgg tagtgccgac gttatcgggc tacgagcatt
      481 tctttttcga acgatgggaa atctgaacga ggcgataagt ctccaagagc agctcatcgc
      541 gcttgacccg ttgaacgtcg gatcttatcg cctcttaggt tttagctttt atgccgcggg
      601 acgctacgaa gacgcgctag ccgcggcacg gaaagcattg gaaatcaacc cacagaccgg
      661 taaagcccac tcccaagctg ctgaaatctt actggcccag ggaaaattac agcaggcttt
      721 ggcggaaacc gagcaagagt cactagcgtg ggccaggctg accgacgaag ccgtcgttta
      781 tcatgcgctc ggacgccagc aggactcgga tgccgcactc gctaaactga ttgcccggca
      841 tcacgaacag ggagcatttc agatcgctct ggtttactct tatcgaaaag aattggacaa
      901 agcgtttgaa tggttggatc gggcctagca gcagcgcgac gctgccatgg tcgtcgtgaa
      961 ggtacatctc ttgctggaga acttgcgaca agatgctcgc tacacggaac tgcttcgaaa
     1021 gttgaacctg ccgttgtaat tcgccggggc tcgctcgccc gccaaaccaa acaattactg
     1081 accaacctca aaatacagat gttgttcttg agtgcgatct ttcggctcat taggagtcgg
     1141 ctgatcaatg cgcagcatcc cgtcgctcgt aagcgcggcc attcgatcaa gaagctgcgg
     1201 cccggtcaca accagttctg aggttccgcg ggcactcgga taacccacgc atcgccaatg
     1261 ttttagacgc tgacagcgaa aagttggaag cggattcaaa aagcccagcc agtatcttgc
     1321 atctacttcc tgactgcttc cttctccagc ttctccaact ctcgagccat ctgttcggct
     1381 tgatcgcgaa gctggtggat gcgctcagcc agtgtggtag cgttcttctc gctgctggct
     1441 gcggcagttg tttgagcggc agcaactact ggcggagccg ctgctcccgt caggtccgcc
     1501 agggcctcgt cgaacgtgtc tcgataaatc agccggtcgc ccatcgccaa aactatcttc
     1561 ttgagctgcg gcatgcgggc ttcggtggct tgaatgtaga tggactcaag gtaaaggaag
     1621 ccgccagtca cggggagagc gatgatgctg ccccgtaaca cgtgcgagcc ttgctggttc
     1681 cacagggtga ggtctttgga aatattctgg tcctgatcga tgcgtgattc gatctgcatg
     1741 gggccgtaca tcaattgttg cttcggcaat tgatagaaga tcagcttacc cagttggtct
     1801 ccatcgcagc gggcggccat ccagccgatc aagttgtcct tgcccctggg gctgaacggc
     1861 agaatcagca agtactcggg ttgtttttcc ccaggcaatg tagccaccac ataagttggc
     1921 gccaagggct cgggctgtcc agactggccg aacagatcgc gcgcaatctc ccaaatgtct
     1981 tctttgttgt agaacacctg agggtcgcgc atatggaaga cccggtacgc ttctgcctgt
     2041 gtgcgaaaaa gggcctccgg ataacgggcg tggcggcgca aatcggcggg catttctgac
     2101 gcagggcgga atagtttggg aaacaatttc tggtaagcct gaataatggg gtcgctggga
     2161 tcgaagacgt ataacgacat ttcgcctgta taggcatcca cggtagcctt taccgagttg
     2221 cgaatgtagt tcgcgccctc ctcaatgcca gcgacgggca gggccgcgga atagggatgg
     2281 gagagagaag tggtgtaagc atcgaccatc cagaccaggc ggccgtcatc ggtaatcacc
     2341 atgtagggat cgggatccca gtgcaggaaa ccggccaagt gggccaatcg tgcctgcacc
     2401 cggcgataga tcatcatgcg gctctgtccg gtcagatatc cggtaaaaac aatgttcggc
     2461 tcgccctggg aaatcgcagc ggcgagtttc agcgggaacg agccgacggg gaagcctcca
     2521 gtgccctggt aggtcgaata cttgttctgg tcgccggatg ggtaatcaaa ctcttcgcga
     2581 gcagtgtgaa caaagacggg atcctgggtc ttctcgccaa aatagatttc cggccgggtc
     2641 agcttaaacc cgggcgactt gatctccgga ggtgcgttct cgatgagcag gacgggcagg
     2701 ccatcaggcg tggttttgtt gacctcggct acgaccgtgc caatgccgtg ggtgtaaatg
     2761 aaacgagggt tgatccaact ctcgctggct tcggcggaca attgcgtgac gtcaatttcg
     2821 cgaggggaca acaacagctg cttgattttg ccgccgatga agtagcgatc aacatcggtg
     2881 tcgggaaacg tgtagtaggg acgcagcgct tgaatctggg taatggttgc gccgtaggcc
     2941 cgcaggtccc acagccgcac attggcgagc agggttgcat cctgtacagg atcgacgacg
     3001 ccctgaccag aaggtgtgaa tgggctttcc gtggcatttc gattgaggcc aaacgcagcg
     3061 gtggtcgcct cgatgtggcg ctcgatataa ggccgttcga tggaaatctc attcggccgc
     3121 acgtagacgg cgcgaacgac agcgggcagc gccagttgca aggcaaaaca tgagaccacg
     3181 aggacggcgg cctgcttgaa tttcgagagc caaaccagcg gcagcgccac caacgcggaa
     3241 acaatgagca gccagcgcaa tggaagagtg actttttcgt cgacgaagtc ggcgccggtc
     3301 atgaaagcgt gagtgttcaa caggagttca aagttgccca gataaaccca ggctgcaaaa
     3361 cccagtaata gaatcacggc aagcacgcgc acgaaggagt tgcgcgtggc acctggcaag
     3421 cgtaggtatg cgccaaaatt aatcggcctt cctggtccat ccattagttg gcgatagcga
     3481 atccgttcgg acaactgcca gccacgagcc gtaccccaga acaacagggc cgaaaggatc
     3541 gccaacccaa acagaaaccc gagcaggtct gaatagaagg gtagatcgaa tagataaaac
     3601 ggcaacgagt gcgaaaagac gtggtcctgc caagcgtccg acggggcagt gattcctcgc
     3661 gagccgaaaa agcgcatgac cgtccagaag tcaattgagg cggacgcgaa ccaaatcgcg
     3721 accaaagcca gacctaccgg aaccaggcga gagtataccg ggtaatcgcg ctgacgtatg
     3781 ccggcgaagt gcaggccacg cgcatgagcc acgtagaatg cgatgaatgc cagcaggcta
     3841 actattgccg caggggcaat gctgtaccac agcatgctga tccacgtgtc tacctgcgcg
     3901 acttctttcc accagttgta ttcaatgatg aaattggcgg cggtcggaat cgcccagccg
     3961 ataaatgcca aagagacgac tacaagaatg aaggtgcgga tacggtaagg acgcatagaa
     4021 gtgatctttg agtgtccagc agaagcgccg cttcgaggtc aaggggacac atcttgagca
     4081 acacggcatc gatgatcgag cgaatgacag gcaagcgtat cacaggaccg acgtagagcg
     4141 agacgtgccg cacggagcga agcgctacgt tgaaggggcc tatgtgtacc cactcatggc
     4201 agcgtatggt tgcacccgcc tcagtagctg ttccccaagc ttctctccta cctcaacccg
     4261 attgaataga cataatcgcc cccggggcgg ctcctttcaa accgctgtat gagacgcgcc
     4321 ccgcagcacc gagcctacct gcatactgac tgtggggcgc atccgataca gcactaaggt
     4381 ttctggccaa tatcggtttg gcagactata aatccacaat cactgttccg cattccagag
     4441 gtcttcttac cttggcgaca aaccgcgatt ccacgcgacg cgctgttttc gtcaactgac
     4501 agccaacttc tgcatggccc gctatttgac cacaatctcc ggcgagctgc gatgccctgg
     4561 gagaagagct cgttagctca gtttttgcct tcagcaatct gaaggtcgag tgcagagcgt
     4621 tcgccgttga tgactactgt tcccgaatcg atcagttgct ggccggtgca atcgtagctg
     4681 gcgaaacagc gcatgatgcg aaagggaaag aagaactcaa cagccaacgg gctggagccg
     4741 tcggttacgt ggatcatgct ggtgaaatta atgccgtcaa aacccggatc gcccgggaag
     4801 tagttcagcg cgggtcgcac aagatgcgaa atatgatcgt caaacgcaag cgctgccaac
     4861 ttgtaccgag aggttccagc ggcagcgttc agctgcgtgg tgatcgagag ttgcaggtac
     4921 gcgccctgac gaaatccgat aaaggtgggc ggcgcatagg ccaggaaatg agcttcttgg
     4981 ttgaggctct tggtcatgcg ttcgatcgcg tcagcgttct ggcgctgcaa ctttgctaag
     5041 tcgtcctgcg tataaattcg cagaatcggc ggggtggtct tcataagacc ggcaga
//
LOCUS       DASRKK010000152         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1377638, whole genome shotgun sequence.
ACCESSION   DASRKK010000152 DASRKK010000000
VERSION     DASRKK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377638"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFC15_03435"
     CDS             <1..180
                     /locus_tag="VFC15_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09246.1"
                     /translation="NPSNAFVPFYTTKPGGSGIGLVLSQQIAEAHGGSIELANRPDTH
                     GCIVKVFLPYRSSPR"
     gene            complement(386..829)
                     /locus_tag="VFC15_03440"
     CDS             complement(386..829)
                     /locus_tag="VFC15_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZM09247.1"
                     /translation="MFRKLVSTSNDFTLTIVRLVLGVTFFMHGAQKMLGWFGGYGFHG
                     TMGFFTQQMGIPTPLAFLAICAEFFGGLGLLVGLLSRIAALGIIVNMLVAIATVHHVN
                     GFFMNWTGQQKGEGFEYHLLAIALAIVVLVKGSGAISIDRAIAGE"
     gene            complement(1193..2632)
                     /locus_tag="VFC15_03445"
     CDS             complement(1193..2632)
                     /locus_tag="VFC15_03445"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZM09248.1"
                     /translation="MPSGFRRIVRLLAVCVLFGSAIFGFAKQDAAKPDEARLKPAYRF
                     ERNHWVYVHLEGSPADIGYQHGYLLAPEIADGVQTVRAVDTYATKRDWNFYRNAAQNV
                     LWKHIDAEYQQELQGIVDGLHAHGVTLDLWDVVALNAMEELPDYYVPWLNKREKRADA
                     PVLKAPGNCSALVATGSMTKDHKPVIAHSNWTDIAVGSRWTVIFDIVPTRGYHMLTDG
                     FPGVIVSDDDFGINSAGLMVTETTITGYDLFDPNGVPEFVRARRAIQYASSIDEYVRI
                     MLDHNNGGYANDWLLADNKTGEIARFEIGLKMHRVWRTKDGYFVGSNFPSDPDFIKKE
                     TDFDPSNMGSSANARHKRWEELMAQSKGQIDVEMAKKFLADHWDSYEGKEDRDERSLC
                     GHAANSPRGVPEWQWPPYSPGGAVTAQAADSTMAKTMSLQARAGYPCGEDFIADKFLK
                     KHPEFDWQRSILKDMKGNPWAEFKAADKQ"
     gene            3124..>3642
                     /locus_tag="VFC15_03450"
     CDS             3124..>3642
                     /locus_tag="VFC15_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09249.1"
                     /translation="MKSVARQSAIRFVMLALLLALCATASLGAQEPPNSAASWKFAVS
                     GDSRNCGDIVMPAIAQGVRRDGASFYWHLGDYRAIYTFDEDYLHTHPASTISNYLAGA
                     WPDFIQHQLQPFGDLPVFLELGNHELVPPMTKGLYIAQFADWLNQPVLQRQRLADNPN
                     DHVLKTYYRWIER"
BASE COUNT          776 a         1062 c         1019 g          785 t
ORIGIN      
        1 aacccgagca acgcttttgt tccgttctat accaccaagc cgggcggttc tggtattggg
       61 ctggtcctgt cacagcagat cgccgaggcc cacggaggtt ccatcgagct tgccaatcgc
      121 ccggatacgc acggctgtat cgtgaaggtc ttcctgccgt accgctcatc gccgagatag
      181 catgacatag ctcgtaaccc ttgcacgggt agtgcgggcc ttcaggcccg cgtccaggaa
      241 aagaagaaga atagcttcag ccgccgaggt cgtcgacctc ggcggctgaa gctaggaact
      301 gcacggcgat cttaacgcgg gcctgaaggc ccgcactgcc cgagatacaa cactctggaa
      361 tcggtctagg cgataatctc ggtgcttatt caccggctat cgcgcggtca atggagatag
      421 cgcccgagcc cttgaccaac accacgattg ccagcgctat cgccaggaga tggtattcaa
      481 atccctcacc cttttgctgg ccggtccagt tcatgaagaa gccattaacg tggtggacgg
      541 tggcgatggc gaccagcata ttgacgatga ttccgagcgc ggcaattcgg ctgagcagac
      601 caaccagaag tccaaggcca ccgaagaatt cggcgcaaat tgccaggaac gccagcggcg
      661 ttggaattcc catctgctgg gtgaagaacc ccatcgtgcc atggaagcca taacctccaa
      721 accagcccag catcttttgt gcgccgtgca taaagaaggt tacgccgagc accaggcgca
      781 cgatggtgag cgtgaagtca ttcgaagtag aaacaagttt gcgaaacatg ggcatcctcc
      841 tcaagattat ttggccgcgt tgtcagcccg gccaatcgag taactagatg cttgttgcaa
      901 cacgtaagtt gcagaattgc gttacatcgg ttgccctcaa accgtgcacc ctgcggcggt
      961 gaatcactta aagcggttcc acaatagatg tatccgcgtc gctcggatag agcggcgctt
     1021 cagcgccgcg ttcgccgcag ccaaacgtcc cgctcaagcc gctgaggtcg tcgacctcag
     1081 cggcttgagc gattttcgct tcctgcctca tacgcgggcc tgaaggcccg cactacccgt
     1141 acgggcggat acactaatcc aggaaccgct ttagccgcga agcaaattgt tctcactgtt
     1201 tgtccgccgc cttgaattca gcccagggat tgcccttcat gtctttcaag atggagcgct
     1261 gccagtcaaa ttcggggtgc tttttcagaa atttgtcggc gatgaagtct tctccgcagg
     1321 gataaccggc ccgagcttgc aggctcatgg ttttcgccat ggtgctgtca gccgcctggg
     1381 ccgtcactgc gcccccggga gaatacggtg gccactgcca ttcgggcacg ccacgcggcg
     1441 agttggcggc atggccacag agggaacgct cgtcgcggtc ctccttgcct tcgtagctgt
     1501 cccagtggtc ggcaaggaat ttcttggcca tctcaacatc gatctgcccc ttgctctgcg
     1561 ccattagctc ctcccagcgc ttgtgacggg cattggccga gctgcccatg ttgctaggat
     1621 cgaaatcggt ttccttttta atgaagtcag ggtcgctggg aaagtttgaa ccgacgaagt
     1681 agccgtcctt ggtccgccag acgcggtgca ttttcaggcc gatctcaaag cgcgcaattt
     1741 cgccggtctt gttatcggcc agcagccagt cgttcgcgta tccgccattg ttgtggtcca
     1801 gcatgatccg cacgtactcg tcgatcgagc ttgcgtactg tatcgcccgg cgtgcccgca
     1861 caaactcagg caccccgttg ggatcgaaca aatcgtaccc ggtgatggtt gtctccgtga
     1921 ccatgagtcc ggcggaattg atgccgaagt catcgtcgct cacgatcact ccggggaaac
     1981 catccgtcaa catgtggtaa ccgcgagtgg gaacaatgtc gaagatgacg gtccatcggc
     2041 tgccaacggc gatgtcggtc cagttgctat gggcgatgac cggcttgtgg tccttggtca
     2101 tcgatccggt tgcaaccagc gcgctgcaat tgcctggcgc cttcaggaca ggcgcgtcag
     2161 cccgcttttc ccgtttgttc aaccagggaa cgtagtaatc gggaagctct tccatggcgt
     2221 tcaacgccac cacgtcccat agatccaggg ttacgccatg ggcgtgaagg ccatcgacga
     2281 tgccttgcaa ctcctgttga tactctgcgt caatgtgctt ccacagcacg ttctgggcgg
     2341 cattgcggta gaaattccaa tcgcgcttgg tggcgtacgt gtccaccgcg cgaacggttt
     2401 gaacgccgtc tgcaatctcg ggtgccagca ggtaaccgtg ctgatatccg atgtcggccg
     2461 gggagccttc gagatgaacg tacacccaat gattgcgttc gaagcgatag gcgggtttca
     2521 agcgcgcttc gtcaggtttc gctgcgtcct gctttgcaaa tccgaagatg gcggagccga
     2581 acagcacaca gaccgcaagc agccgaacaa tccggcgaaa tccagatggc atagattctc
     2641 caatgggcat atgaccgcag gggcacaagg gtttttgttc ccggctaacg cggcgcgaaa
     2701 gcgccgctct accgatgctc gcaggataca gtaactttag gaatcaccct cctgaattat
     2761 tcatgaggat ctcacgcttt gaagggacac ggcttcagcc gtgccgtacc tgccatcgtt
     2821 aatggtggct ttagccactg aggaacttcg gtgttgagac gaagtgcgag ttccctcagg
     2881 ggctgaagcc caactctcga ggcacacgta acggcacggc tgaagccatg ccctttcaaa
     2941 gcgtgccact tcagttagcg aaacagatga acaatccagg ctatcccgaa tatcaatgcg
     3001 agacgtggga tggaattctc cacggagaac atggcaatca gttaatgtat ggaagtgaca
     3061 agatgccata cggtctggcg ccgtgaggga aacatgctcg attgctggga gagaatacca
     3121 atcatgaagt cggtagccag acaatctgca attcgattcg taatgctggc tctgcttctg
     3181 gccctgtgcg cgaccgcgtc actgggcgcg caagagccgc ccaactctgc ggcaagctgg
     3241 aaattcgctg tgtcgggcga ctcgcgcaac tgtggcgaca tcgtgatgcc cgcgatcgcc
     3301 cagggagtcc gtcgcgacgg agcgtctttc tattggcatc tcggcgacta tcgggccatc
     3361 tataccttcg acgaggacta cctccacact catccggctt ccaccattag caactacttg
     3421 gccggtgcct ggcctgactt cattcagcac caattgcaac cgttcggaga tttgccggtg
     3481 ttcctggaat tgggcaacca tgaactggtt ccacccatga cgaagggact ttatatcgcg
     3541 cagttcgccg actggctcaa ccaacccgtg ctgcagcggc agcggctggc ggacaatccc
     3601 aacgaccacg ttttgaagac gtactatcgc tggatcgaac gc
//
LOCUS       DASRKK010000153         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_302676, whole genome shotgun sequence.
ACCESSION   DASRKK010000153 DASRKK010000000
VERSION     DASRKK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302676"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFC15_03455"
     CDS             complement(<1..301)
                     /locus_tag="VFC15_03455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM09250.1"
                     /translation="MEKQLAEFEAAGVRPVAISVDPPEVSRDLCRKAGYTFTVLSDPN
                     AEVTRRYHLLHAGGGPDGHDIARPAEFLVDSSGMVRWVNFTEDVRVRARADEMLAA"
     gene            complement(329..502)
                     /locus_tag="VFC15_03460"
     CDS             complement(329..502)
                     /locus_tag="VFC15_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09251.1"
                     /translation="MPTSAGAPQVGQRVPDFTLADTNDQPVSLAQLLAQAANDSKAMP
                     AKSVLLIFYRGYW"
     gene            complement(801..1055)
                     /locus_tag="VFC15_03465"
     CDS             complement(801..1055)
                     /locus_tag="VFC15_03465"
                     /inference="COORDINATES: protein motif:HMM:NF022969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HZM09252.1"
                     /translation="MNRVPDTELVHIPGGTLITATPEEGRALAMTIARHTIHNIQPDL
                     DVLKGGRPSYSSNPEGLIAASQVVAIEFQTIAAANNYWRR"
     gene            complement(1175..2110)
                     /locus_tag="VFC15_03470"
     CDS             complement(1175..2110)
                     /locus_tag="VFC15_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM09253.1"
                     /translation="MSKTEAYRNPAAEAFRMPDAPTLTARVLRKSTMAATELKYDQRN
                     FGTTSPMAREDAYLIALHLRACHDHDLYFDGRIVRPKNYFAGVTSISDLRQEPVADIR
                     DPFHCIVVHLPRRAIDAMTDEAGAARVGDFRHEPGVSTDDPVVRHLLASLLPALMKPE
                     EAQPLFLDHLSLALIAHLAHTYGGMTAIARLPRGGLAPWQERRVKELMSDTLQEEVPL
                     SRLANECGLSVRHFARAFRRSTGTSPHRWLLQRRVERAGEMLGNRAISLAGIALACGF
                     ADQSHFSRVFTATVGVSPGAWRRANGVTSRGRGNG"
     gene            2381..>3112
                     /locus_tag="VFC15_03475"
     CDS             2381..>3112
                     /locus_tag="VFC15_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM09254.1"
                     /translation="MGQRPDSIVLINGLWMTALSWENWVKRYTGKGFQVIAKSWPGMD
                     VDIEQLRRDPTPIAQLGITEIVDHYEKIIRGLDSPPIIIGHSFGGLMTQILIDRGLGA
                     AGVAIAPAPVKGIIFLPFSTLKVTFPALSNPSNNHRAVPLTPEQFHYAFTNNLSEVDS
                     LAVYQRYAVPGPDHVLFQAAFANFNPHAATAVDFHNNDRAPLLLISGGTDHVSPSSVV
                     EANFKLYEKSTAVTEYREFPERTHYT"
BASE COUNT          685 a          939 c          836 g          652 t
ORIGIN      
        1 cagcagccaa catctcgtcc gcacgcgctc gcactcgaac gtcctccgta aaattgaccc
       61 agcgcaccat ccccgatgag tccaccaaga actcggctgg tcgggcgatg tcatgcccgt
      121 ccggcccgcc gcccgcgtgc agcagatggt aacgacgagt cacctccgcg ttcggatcgg
      181 aaagaacagt gaacgtatag cctgccttcc tgcacagatc gcgcgagacc tctggcgggt
      241 ccacgctgat cgccaccggt cgaacgcccg ccgcctcgaa ttcagctaat tgtttctcaa
      301 ttcctcgtaa ctcgaggttg cagaacggtc accaatatcc acgatagaaa atcagcaaca
      361 ccgatttagc cggcatggct ttcgaatcgt tggcggcttg tgccaaaagt tgggctaacg
      421 acaccggctg atcattggtg tcggccagcg tgaagtcagg caccctctgt cctacctgcg
      481 gcgcacccgc agacgttggc atggcccgcg cgctcaagga cgcgaagatc gccacgccca
      541 ctagcagcag cgagaccaaa gagaggaccg agcttagaat tttccttgat cactcggaag
      601 aacgtggatc agacggaagt tcggtactct gaaagtgcgc tagtctaacc ttttcggctt
      661 gactcatgta aaccgcaacg aggcggaagc gcttatgcac tcgcttataa cctgcgtccc
      721 caagcaacac gcagaaaaaa gtcacaggct gtgagcgaag tcgaaggctc ctggccatcc
      781 ttcgtttaat ctgacggcct tcagcgtctc caatagttat tcgccgcggc aatcgtctgg
      841 aactcaatcg ccacgacctg actggcggca atcagaccct ctggattcga agagtagctc
      901 ggtcggccac cctttagaac atcgaggtcc ggctggatat tgtgaatggt gtggcgggcg
      961 atcgtcatcg ctaatgcacg tccttcttca ggcgtagcgg tgattaatgt gccgccagga
     1021 atatggacca gctctgtatc aggtacgcga ttcacggtgt ctcccccttg tgcgccgcga
     1081 taatatcaaa actcccggag caaaacatca atatcgagcg aaaatgaggt cgatgccgga
     1141 tgacccatct cgcaagagaa tctggcgctg tgacctatcc gttacctctg ccacgcgacg
     1201 tcacgccgtt cgctcgccgc catgcccccg gacttactcc aaccgtagct gtgaaaacac
     1261 ggctgaaatg actctggtcc gcaaacccgc acgccagagc aatgccggcc agggagatcg
     1321 cccgattgcc cagcatttcg ccggcacgct ccacccggcg ttgcaacaac cagcggtgag
     1381 gtgaagttcc agtcgatcgt cgaaacgcac gcgcaaagtg cctgaccgat aagccgcact
     1441 cgttggccag acggcttagc ggaacctctt cctgcaacgt gtcgctcatc agttccttca
     1501 cccgtcgctc ctgccagggc gcaagtccac cgcgcggcaa tctggcaatc gccgtcatcc
     1561 cgccgtaggt atgggccagg tgggcgatca gcgctaacga aaggtggtcc agaaatagtg
     1621 gctgcgcttc ctcgggtttc atgagggctg gcagcaacga cgcgagcagg tgtcgcacta
     1681 cgggatcgtc agtgctcact cccggttcat ggcgaaaatc gccgacgcgt gccgcgccgg
     1741 cctcatccgt catggcatcg atcgccctgc gcggtaggtg aaccacgatg cagtggaatg
     1801 gatctcgtat atcggccacc ggctcttgcc gtaaatcgga aattgacgta acgccggcga
     1861 agtaattctt cggtcgcaca atccgcccgt cgaaataaag gtcgtgatcg tggcacgctc
     1921 gcagatgtaa cgctatcaag tacgcatcct cgcgggccat tggagacgtc gtgccgaagt
     1981 tccgctgatc gtacttaagt tcagtggcag ccatcgtgga cttccgaagc accctcgccg
     2041 tcagcgttgg cgcgtccggc atgcggaaag cctctgccgc tggattgcga taagcctccg
     2101 ttttgctcat tcgacggttc ctcgtcttta tcgctggctc gagagagcgt cacgatccca
     2161 ccggatatcc acggcttaaa cgcggattgc ggttccagcc aaggttctct gtgtctgttt
     2221 tctacaaaag ccggcctgat cagacaagac ccagtctccc ctccggctcg attctgcatt
     2281 ggatgcgcga ggtcttcgga ggtcgcgaat ttgggcagag ctctgtccgg aagccgacct
     2341 tgaacctatc cgcaagtcat gtatcagaga ggataagaca atgggtcaac gaccagactc
     2401 gatcgtgttg attaatggac tttggatgac ggcccttagc tgggagaact gggtcaaacg
     2461 ttacaccggc aaaggatttc aggtgatcgc caagagttgg cccggcatgg acgtcgatat
     2521 tgagcaactg cggcgtgacc cgacgccaat cgcccagttg ggcatcaccg agatcgtaga
     2581 ccactacgag aaaatcatcc gcggattgga ttctcctcca atcattattg gccattcttt
     2641 cggcggattg atgacccaga tattgattga ccgcggatta ggtgccgccg gtgttgcaat
     2701 cgcgccagcc ccagtgaagg gcatcatctt cttgccgttt tccacgctga aggtgacgtt
     2761 cccggcactc agcaatccat cgaataatca tcgcgcagtg cctttgacgc cggagcaatt
     2821 tcattacgcc ttcaccaaca acctcagcga ggtagactcg ctggcggtgt atcagcgata
     2881 cgccgtgcct ggtcccgacc acgttttgtt ccaggccgct tttgccaact tcaatcccca
     2941 tgcggcgacg gctgtggatt ttcataacaa tgaccgcgct ccactgctgc tgatttccgg
     3001 aggaacggat cacgtatcgc catcctcggt tgtcgaagcg aatttcaagt tgtacgaaaa
     3061 atccacggcg gtaacggaat acagggaatt ccccgagcgc acacactaca ct
//
LOCUS       DASRKK010000154         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_400830, whole genome shotgun sequence.
ACCESSION   DASRKK010000154 DASRKK010000000
VERSION     DASRKK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400830"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1598)
                     /locus_tag="VFC15_03480"
     CDS             complement(342..1598)
                     /locus_tag="VFC15_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM09255.1"
                     /translation="MAQAAGHADRNLPLRNYCTGLLLDGERKSVEPMAARLAPDHVQS
                     MHESLHHFVALSPWSDADMLRQVRNYVLPAMQKKGPVTAWVVDETSFVKKGTHSVGVA
                     RQYCGRLGKKENCQVAVSLSVATAAGSLPIAWRLYLPEEWASDPERRRETRVPEPVEF
                     QTKPQIALAQIQQAVKDGVPLGVVLADEAYGSNREFREGVADLKLDYSLAVRSTTTVW
                     ALERQPLPPAPWRGIGPKATLMRRDQTHQPITVKQLAQELLEKAWREVSWRQGSKGTL
                     RSRLAALRVRPAYGDDRKGGLQPEQWLLIEWPAGAAEPSGYWLASLPAKLSLKRLVGI
                     SKHRWVIERDYEELKQELGLTHYEGRNWRGFHHHATLCIAAYGFLMAERNRFSPSAHV
                     GHLGLCAAPIPPHFRPRGAKGSRPTA"
     gene            1816..>2080
                     /locus_tag="VFC15_03485"
     CDS             1816..>2080
                     /locus_tag="VFC15_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09256.1"
                     /translation="MRLLVAVFAACFAAQAQIPAQDARNIDLPNTDTHFAAKTYATLA
                     EWQARREFLRKQILSAAGLMPLFPKSDLHPQVFGRIEHPDYSIE"
BASE COUNT          409 a          668 c          591 g          412 t
ORIGIN      
        1 tgctacgggg caactgggag gcgctgggag aactggaggg gttggcatgg tggctcttca
       61 tcgtggcctc tctctacgtt gatctgaccg ctcgccattg gaagaccccc cggacggcgg
      121 cacatgcggg aagagctgaa ccggtctaac cagaacgtca taaataattg cgcatccgct
      181 ctctgacgtt cgcggctccg aatcggcgcc gtacggagcc gcgcgcgtaa ttctactgtg
      241 tcataaatta tacgcgcaag cgacagcatt cggggcagca cgggagctgc tgcaccagga
      301 atcgggcgat cttgatacgg agcgtcgcga tggaatgggg attatgccgt tgggcgcgag
      361 cccttcgctc cccgcggccg gaagtgggga ggtatcgggg cggcgcataa cccaagatgg
      421 ccgacgtggg ctgaggggga aaaacggttc ctttccgcca tgaggaaccc gtaagccgcg
      481 atgcataaag tggcatggtg atgaaatccc cgccagttgc gaccttcata gtgagttaac
      541 ccgagttcct gtttgagttc ctcgtagtcg cgctcaatca cccaccggtg cttgctgatc
      601 ccgactagcc gtttcagcga gagtttcgct ggcaaactcg ccaaccagta gccgctcggc
      661 tcggctgctc cggcgggcca ctcgatcagc aaccactgct ccggctgtaa gccccctttg
      721 cggtcgtcgc cgtacgcggg gcgaacccgc aaggcggcca agcgggaacg caacgttcct
      781 ttgctgcctt ggcgccagga cacttcccgc caggcttttt ccaacaactc ctgcgccagt
      841 tgcttcaccg taatcggttg gtgtgtttgg tcccgacgca ttagcgtggc cttcggccca
      901 atgcccctcc aaggcgcggg gggcaaaggc tgccgctcca gcgcccacac cgtagtggta
      961 gatcttaccg ccagcgaata gtccagcttc aggtccgcca cgccctcccg gaattcgcga
     1021 ttggatccgt atgcttcatc ggccagcacc accccgagcg gtactccgtc cttcaccgct
     1081 tgctgaatct gtgccagcgc aatctgcggc ttggtctgaa actccaccgg ctcgggaacc
     1141 ctagtttccc gccgccgctc tgggtcgctg gcccattcct ccggcagata caaccgccag
     1201 gcaatcggca gactcccggc cgcagtcgcc accgataaac tcaccgccac ttggcagttc
     1261 tccttcttgc ccagccggcc acaatattgg cgtgctactc caaccgaatg agtgcccttc
     1321 ttgacaaagc tggtctcatc cacaacccat gccgtcaccg gccccttctt ctgcatcgcc
     1381 ggcaggacgt aattccgcac ttgccgcaac atgtcggcat cgctccaagg cgacagggcg
     1441 acgaaatgat gcagcgactc gtgcatgctt tgcacatgat cgggagccag gcgcgccgcc
     1501 atgggctcaa cgctcttgcg ttcgccgtcc agcaacaagc cggtacagta attccgcaga
     1561 gggaggttgc ggtcagcatg ccccgcggct tgcgccagcc agtccatata tgccgcgagg
     1621 cgtttctctt gtggtgctgg cgccggggtt ttcgcagcca cgaaaacatt atatgacaaa
     1681 tttgttgttt atgacacagt agaagtaagc aagcggttgt ggcgcaaaca ataacgtcgc
     1741 catgtttaga atgggcgatt ggatgtacaa tcgaaggcgg ccaggaagcg aaaacggaaa
     1801 tcctggctta taaccatgcg cctcctggtt gcggttttcg ccgcctgttt cgccgcgcag
     1861 gcccaaatcc ccgcgcaaga cgcgcgcaac atcgacctcc ccaacacaga cacgcacttc
     1921 gccgccaaga cgtacgcgac cctcgccgaa tggcaggccc gcagggaatt cctgcgcaag
     1981 cagatcctgt cagccgccgg ccttatgccg ctctttccga agagcgacct gcatccgcag
     2041 gtcttcgggc ggatcgagca tcccgactat tccatagaga
//
LOCUS       DASRKK010000155         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1280375, whole genome shotgun sequence.
ACCESSION   DASRKK010000155 DASRKK010000000
VERSION     DASRKK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280375"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFC15_03490"
     CDS             <1..207
                     /locus_tag="VFC15_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09257.1"
                     /translation="TPGYVITPLQGLKEKAGSGIAVNYAPPADIDASVKAARESDVAV
                     VFVGNHPTCDAGWAKGRPKNKITW"
     gene            381..1166
                     /locus_tag="VFC15_03495"
     CDS             381..1166
                     /locus_tag="VFC15_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09258.1"
                     /translation="MRTESFHADDLAKALQRSKIATMPELKRALGTEVDVTVFRKLKQ
                     LAYRTSYSHRGSYYTLDETAHFDESGLWSFQSVWFSRQGTLLDTAQAFVENSSAGHFA
                     EELDHLLHVGTKEPLLRLVQHERVAREPVGGLYLYCSMNPVLREHQLLARHVLQAEPN
                     LASSLAKAEVVSDELKAAIILFSSMLNEKQRRLYAGLESLKLGYGGDQRIAEFIGMDP
                     HTVAKGRRELLEQDLEIDRIRQAGGGRKPLEKKHPKSSPESKN"
     gene            1166..2089
                     /locus_tag="VFC15_03500"
     CDS             1166..2089
                     /locus_tag="VFC15_03500"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HZM09259.1"
                     /translation="MQHDTAGDPITGVKWSRRTTRKIAEQLTALGIVVSKNTVGRLLK
                     QLKFKLRVNRKQIASTKNPDRNQQFLYIGQQRERFVSQGLPIISVDTKKKEQIGNFKN
                     AGAKWDREPVLVRDHDFRSDADGMAIPYGIYDTQANRGAVFVGTSHDTPAFAVDAIAQ
                     WWLKEGCQRYPVARELFILADGGGSNGPRCRAWRKAIQDTICTPLGLTVTVSHYPPGA
                     SKWNPIEHRLFSPISKNWAGEPLTDIDKILKFIRTTKTESGLTVNAYLIPENYDTGIK
                     ISDAEMRQLNLAPHEMLGRWNYTVRPTQNVN"
BASE COUNT          525 a          642 c          606 g          400 t
ORIGIN      
        1 accccgggct acgtgatcac gccgctacag ggattgaagg agaaagccgg cagcggcatc
       61 gccgtcaact acgctccccc ggccgatatc gatgcgtccg tcaaagccgc cagggaatcc
      121 gatgtggcgg tggttttcgt aggcaaccat cccacctgcg acgccggctg ggctaaggga
      181 cggcccaaga acaagatcac atggtaaagc gcagatcttg cagtttggtc attcgctaag
      241 gctcttcggc ccagccgggc cgtaatcgtt caacatcaat attggcagta ggccagaccg
      301 tccggtcgca gtggacagga ggccgttatt ttgttagagt tctacctgaa gagtgatttg
      361 acaggtagac ttctaacatt atgagaactg aatccttcca tgctgatgat ctggcgaagg
      421 cgctacagcg gagcaagatc gctaccatgc ctgagttgaa gagggcgttg ggaacggagg
      481 tggatgtcac cgtcttccgc aagctgaagc aactcgcgta tcggaccagc tactcccatc
      541 gtggcagtta ctacacgtta gacgaaaccg cgcacttcga cgagagcggc ctgtggtctt
      601 tccagtccgt ctggttctcc cgccagggca ctctgctgga tacggcccag gcttttgtcg
      661 agaattcctc ggcaggacat tttgcggagg agttggatca cttgctgcac gtggggacca
      721 aggagccgtt gctgaggctg gtgcaacacg agcgcgttgc tcgcgagcca gtggggggat
      781 tgtatctgta ctgttcgatg aatccggttc tccgcgagca ccaactgctg gcccggcacg
      841 tgcttcaagc cgagccgaat ctggccagca gcttggccaa ggcagaagtg gtgtccgacg
      901 aattaaaggc agcgattatt ctgttctcca gcatgctgaa tgaaaagcaa cgccgcctgt
      961 acgcagggct ggagtcgctc aaactcggct acggcgggga tcagaggatt gccgagttca
     1021 tcggcatgga ccctcacacg gtggccaagg gccggcgcga actgctggaa caagacctgg
     1081 aaatagaccg catccgccaa gccggcggcg ggcgtaagcc gctggaaaaa aaacacccga
     1141 agtcatctcc cgaatcgaag aactgatgca gcacgacact gccggagacc ccatcacggg
     1201 ggtgaagtgg agccggcgaa cgacgcgcaa gatcgctgaa cagctcaccg ctctgggaat
     1261 cgttgtgagc aagaacaccg tgggccgcct gctcaaacaa ctgaaattca aactccgcgt
     1321 gaaccgcaaa cagatcgctt ccaccaaaaa ccccgatcgc aatcaacagt tcctttacat
     1381 cggacagcaa cgagaacgat ttgtcagcca aggcctgccc atcatcagtg tcgatacgaa
     1441 gaagaaggaa caaattggca acttcaaaaa cgccggcgcc aagtgggatc gcgaacccgt
     1501 cctggtaagg gatcatgatt ttcgctcgga tgccgacggc atggcgatcc cctacggcat
     1561 ttacgacacg caagccaacc ggggcgctgt gttcgtgggc accagccacg acacgccggc
     1621 ctttgccgtc gacgccatcg ctcagtggtg gttaaaggag ggatgccaac gatacccggt
     1681 cgcccgcgaa ctattcatcc tggctgacgg cggaggaagt aacggcccgc gatgccgcgc
     1741 ctggaggaaa gcgattcagg acacaatctg cactcctctc ggtctcacgg ttaccgtcag
     1801 ccactatccc ccgggcgctt ccaagtggaa tcccatcgag caccgcctgt tcagcccaat
     1861 cagcaaaaac tgggctggcg aacccctcac cgacatcgac aagatcctca agttcatccg
     1921 taccaccaaa acggaatccg gccttaccgt caatgcgtat ctcatcccag agaactacga
     1981 caccggaatc aagatctcag acgcggaaat gcgtcaactc aacctcgctc cccacgaaat
     2041 gctcgggcgc tggaactata ccgtacgccc cactcagaat gtgaactaat tcttgggcac
     2101 cggcttactc ctcaaatgcc ccgcaatatg gcaacttaag gtatgccggc agagcgcacg
     2161 cacgagtatc gga
//
LOCUS       DASRKK010000156         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_10101, whole genome shotgun sequence.
ACCESSION   DASRKK010000156 DASRKK010000000
VERSION     DASRKK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10101"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1010..1336)
                     /locus_tag="VFC15_03505"
     CDS             complement(1010..1336)
                     /locus_tag="VFC15_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZM09260.1"
                     /translation="MSTATITTEFRTLDAGSRLPDNYVNPYYLEDLKRRVAVARVGGK
                     LFAFDDLYEGCPLSGGLLTGTTLMSQCDGSQFEVTSGAVLRGPAKAPLKVYEVRELDG
                     KIQLRI"
     gene            complement(1373..>3052)
                     /locus_tag="VFC15_03510"
     CDS             complement(1373..>3052)
                     /locus_tag="VFC15_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZM09261.1"
                     /translation="VAALRATEKGYRVGVLESGRRWLDEDIPTTSWDVRKFAWQPEAE
                     MYGIQRMEFLDDVLVLCGAGVGGGSHVYGNTLYVPPKKFFDAPEWAGITDWAGELAPY
                     IDQATRMLGVVRVPYMDTQVDRLMREVAQDLGKGDSYNKAPVGVYFGTPGVEADDPYF
                     GDEGPRRRGCINCGNCMIGCGRGSKNKLTVNYLYLAEKHGAQVFELHEVHEIKPLPGG
                     GFEVHARHPGWMQRAADLHHHTYTADQVIVSAHAYGSAKLLHHLKHVGMLPGLSDQLG
                     KGARTNSEQLISITLPYANWKHDPEKVHITPGSVSITSGVWPDAQTSIEPVYYGVSSD
                     IIAFALTYHQEGAQKHPVEGWLKELIEHPSKVLSRFDARHWSERQAVMLCMQTSDTSI
                     ELFWKDDMLRSRHGSGTPPSTHIPVVEDFVDRLARKMHGDQAALWFEVLNRTASAHFI
                     GGMSIGESTDDGVVDPYQRAFGHPGLHVMDGSVMPANPGVNPSLMITALAERAMSFWP
                     NKGDPDLRPSLGSTYQRFPAIMPHRPIVPDTAPGALRLNARKEDIIPLYPY"
BASE COUNT          622 a          913 c          869 g          648 t
ORIGIN      
        1 gtagcgaccg tcccagcggc cccagcgcaa ttacctgctg tcgtttcgtt tcgctcaaga
       61 cattccccat gccaaccgag gctacctgca ccccagtcgt agcttctgtc ttgggcctct
      121 aacatggccg cttttgggtg acccccgagg ctgacgttac ttggtagaag gccagctgct
      181 tccgtaagaa caggttctcc gcaagcagag ctgaacgcgc gaccgaacac aggaacgcag
      241 ggatgtgctg agctccagac agacgccaaa gcccgcgcgg atgagggagg agaattgcag
      301 cccactgagc attgtgtcta attgtatttg tgcccaagcg atcgcgatcc attagaattc
      361 agcgtgttag atcgtaacgt gacggcggaa tttggccaat tggcggagga gcctgactgc
      421 cccgtgtttt caatgagttc agaggtccga cggaatattt gcgcaacaca aggcacattc
      481 tcggttccca tcaccaagca ccagctttga aattcagcca tagcgatggg accttattcg
      541 gtttggcggt aactaccaga atgtctctgc ggcgtggcag tattcccgct cggcaaacct
      601 aattaatctg gtcgtgatgc agtttgaatt tacgcgctta accaattgcc ttcagctaga
      661 aaattgagat cctctgccat gcgatagcgc cgccctataa gtactacagc actgccggtc
      721 ctttatcatg ccggaaggat gaggtatatg agcgacctcg aaatgaatgt tccgcaaaag
      781 cattcagtcg attatctcca caccgaagcg aaaacagcga tcgcgtcgaa ttagcagtgg
      841 tgggccgcac caatagccgt gtttctccct tgtactggca atgggcgctc accaacgaga
      901 aacgctactc cgcagcgtct caatccgaag tctttcatct ggattggtag cgccaacgac
      961 attcagccgc agccttcgat ccgcttcctg ggccactccc ccgccaacgt cagatccgca
     1021 gctgaatctt accgtctagc tcgcgtactt catacacctt gaggggcgct ttcgccggtc
     1081 cacgcaacac ggcgccgctc gtgacttcaa actgcgagcc atcacactgt gacatgagcg
     1141 tggtgccggt gagcagtccg ccggacagcg ggcagccttc atacaagtcg tcgaaggcga
     1201 agagtttccc gccgacgcgt gccaccgcca cccgccgttt caagtcttcc aggtagtagg
     1261 ggttgacgta attgtcgggc agcctggatc cggcatccaa cgtacgaaat tcggtcgtga
     1321 tggttgcagt actcatgatc ggttctcctt ttcgaatggt cgtcgtttcg agttagtagg
     1381 gatagagcgg gatgatgtct tccttccggg cgttaagccg aagcgcgcct ggagcggtgt
     1441 ccggcacgat gggtctatgc ggcatgatcg ccggaaatcg ttgatatgtc gaaccaagcg
     1501 atggccgcag atctggatcg cccttgttcg gccaaaagga catcgcgcgt tcggccagcg
     1561 cggtgatcat cagcgacggg ttcacgcccg ggttggcggg catcacactg ccatccataa
     1621 cgtgaaggcc gggatgtccg aacgcgcgct ggtagggatc caccacgccg tcgtcggtgc
     1681 tttcaccgat ggacataccc ccgatgaaat gcgccgaagc agtgcgattt aggacctcga
     1741 accacaaggc cgcttggtcg ccgtgcatct tcctcgccaa cctgtcgacg aagtcctcca
     1801 ccaccgggat gtgggtggag ggcggcgtcc cgctaccgtg acggctgcgc aacatgtcat
     1861 ccttccagaa caactcgatt gacgtatcgg aggtttgcat gcagagcatc acagcctggc
     1921 gttccgacca gtggcgagcg tcaaaccggc tcagcacctt ggatggatgc tcgatcaact
     1981 ccttgagcca accctccacg ggatgtttct gggcaccttc ctggtgatag gtcagcgcaa
     2041 aagcaatgat gtcgctgctc actccgtagt aaaccggttc aatgctggtt tgagcgtccg
     2101 gccatacacc cgaggtgatg gacacagacc cgggcgtgat atgaactttc tccgggtcgt
     2161 gtttccaatt ggcatagggc agcgtgatcg agatcaattg ttcggagttc gtgcgcgcgc
     2221 ccttgcccaa ttgatccgac aggcccggca gcatgcccac atgtttcagg tggtgcagca
     2281 gtttggcaga gccgtaggcg tgcgcggaga caatcacctg atcggccgtg taagtgtggt
     2341 ggtggagatc cgccgcacgc tgcatccagc cggggtgacg ggcatgcact tcaaatccgc
     2401 cgcccggcag cggtttgatc tcgtggacct catgcagttc gaaaacctgg gcaccgtgtt
     2461 tctccgccag gtaaagatag ttcacggtga gcttattctt ggagccgcgt ccgcacccga
     2521 tcatgcaatt cccgcagttg atgcagccac ggcgccgggg accttcgtcg ccgaaatagg
     2581 gatcgtcggc ttccacgccc ggcgtgccga aatagacgcc gaccggcgcc ttgttgtagg
     2641 aatcgccctt ccccaggtcc tgcgcgacct cgcgcatgag tcgatccacc tgcgtgtcca
     2701 tgtaggggac gcgtaccacc ccgagcatcc gggtcgcctg gtcaatgtag ggggccagtt
     2761 cacctgccca gtctgtgatt ccggcccact cgggggcgtc gaagaacttt ttcggcggga
     2821 catagagcgt gttgccatag acgtgcgatc cgccgccgac gccggcgccg cacagcacca
     2881 gcacgtcgtc gagaaactcc atacgctgga tgccatacat ttcagcctct ggctgccagg
     2941 cgaatttgcg gacatcccag ctggtcgtgg gaatatcctc gtcgagccag cgccgccccg
     3001 actcgagcac gcctaccctg tagccctttt cggtggcccg gagtgcagcc ac
//
LOCUS       DASRKK010000157         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1280868, whole genome shotgun sequence.
ACCESSION   DASRKK010000157 DASRKK010000000
VERSION     DASRKK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280868"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VFC15_03515"
     CDS             complement(<1..1121)
                     /locus_tag="VFC15_03515"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZM09262.1"
                     /translation="MNQRQIVLCTPVRTAIGTYGGGLKSVPATDLGATVVRAVLTRSK
                     LDGSHVGTVVMGNVIQAGNKMNPARQSAVNGGIPVDVPAMTVNRVCGSGAQAIASAAQ
                     EVMLGMLDSAVAGGMENMDMAPYLVGGGRWGYRLGDAQIYDSLLRDGLNDAFSGEHSG
                     WHTEDLAKSHQITREAQDRWAERSQKRFSTAQAAGKFKDEITPVEVHGHKGPTVFDKD
                     EHNRPDTTLESLAKLHPAFRKDGTITAGNAPGLNSGGSAMIVAERGWAEQHGLKPMAR
                     LVSYGVGAVEPGMFGLGPIPAVRQALERANWKIGEIERIEINEAFAAIAVAVTRELGL
                     SEDIVNVEGGAIAHGHPLGATGAVLTTRLLYSMKRDGIKR"
     gene            1427..1873
                     /locus_tag="VFC15_03520"
     CDS             1427..1873
                     /locus_tag="VFC15_03520"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZM09263.1"
                     /translation="MLDSTTKTTTTKPTTSPKMDATQALRETVESGSAQAKEAFEKMS
                     AATAEATASIKNSYSSAVKGVQDYNTKVIEFANANTEAAFAFAKTLSGVKSPSDFIEL
                     STKYSSKQLETLTEQTKELAELAQKVTLATTESFKTGIPQAFGQST"
     gene            2069..2314
                     /locus_tag="VFC15_03525"
     CDS             2069..2314
                     /locus_tag="VFC15_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZM09264.1"
                     /translation="MARLAMLTPQQVRVLMMLSEGLFNKQIAFQLGVSEATVKAHVAA
                     ILQTGSCLGVYSQDVKSIGRQSTEQSAANALSGYALP"
     gene            complement(2250..3323)
                     /locus_tag="VFC15_03530"
     CDS             complement(2250..3323)
                     /locus_tag="VFC15_03530"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM09265.1"
                     /translation="MSVPAFWPMAMAAKLVEEGAELYAKNLKFAEEEIKIHDDLRPTL
                     ATPNQVRLDLRTMVLRDYGKPGGLPTLVDAPHAGHTAMIADYHKGQSLVETLLANGIG
                     HVALTDWKSATEDMKDLEIDNYLAEVIIAIDDLGGRVNLVGLCQGGWVAAMVAARFPD
                     KVNSLVLAGAPIDTDAGNGPIKRMVHESPASFYEELVALGGGLTKGKYMLQGWKNMHP
                     EQHYIQDHVDLYEHIDDPAYLAKEETFESWYENPIDLPGRWYLQVITQIFKENRLAKG
                     KFIGLGRQLSLHDITCPAYLLAGADDDITTPEQVLDAAKYLGTPQDRIVQKTVPGGHI
                     GLFMGARTLKEHWPPIARWIAAQ"
BASE COUNT          776 a         1154 c         1039 g          821 t
ORIGIN      
        1 cgtttgatcc catcccgctt catcgaatag agcaagcgtg ttgtaaggac ggctcccgtt
       61 gcgccgagcg gatgtccgtg agcaatggcg ccgccttcga cgttgacgat gtcctcagat
      121 aggccgagct cccgcgtcac cgcgacggcg atagcggcaa aggcctcgtt gatctcgatg
      181 cgttcgattt cgccaatctt ccagttcgcg cgctctagcg cctgtcgaac cgccggtatc
      241 ggaccaaggc cgaacatgcc cggctcgacc gcgcccacac cgtaggagac aagccgcgcc
      301 atcggcttaa gtccgtgttg ttcggcccag ccacgttccg cgacgatcat cgcagagccg
      361 ccactgttta gcccaggcgc gtttccagcc gtgatcgtcc cgtccttgcg gaaggctgga
      421 tgaagcttag cgagactctc aagcgtcgtg tccggccggt tatgctcgtc cttgtcgaag
      481 acggtcggtc ccttgtggcc atgcacttcg accggcgtaa tctcgtcctt gaactttccc
      541 gccgcctgcg cggtcgaaaa gcgcttttgc gaccgttcag cccaacgatc ctgcgcctca
      601 cgtgtaatct ggtgggattt agccaagtct tctgtatgcc agcccgagtg ctcgcccgag
      661 aacgcatcgt tgaggccgtc ccgcagtagg ctgtcgtaga tttgtgcgtc gcctagccga
      721 tagccccatc gtccgccgcc cacgaggtaa ggcgccatat ccatgttctc catgccgccg
      781 gccaccgcac tgtccagcat accaagcatg acctcctgcg ctgcggaggc gatggcctgg
      841 gcgccggagc cgcacacgcg gttgaccgtc atcgctggaa catcgacggg gatcccacca
      901 tttacggccg actgcctagc cgggttcatc ttgttgcccg cctggatcac gttccccatc
      961 acgacggtgc ccacatgcga gccgtcaagc ttggaacgcg tcagcacagc acgcacaacc
     1021 gtggcgccca ggtcggttgc gggcaccgat ttgaggcctc cgccgtaggt cccgatcgcc
     1081 gtacgaacgg gagtgcatag aacgatctga cgctgattca tgctcttcac tcctcactcc
     1141 gggctagccg agacggcgaa ccgatcaacc acaatgtttc aacgctttgc atcacacatg
     1201 tttccatgcc gctgatcagg caaccccgct ggttagaccg cgggctaccg ttgaataagc
     1261 ctatttttga ctgaactttc gtcgacctcg cgcgtttaaa ctgacagaaa ctctgctgcg
     1321 aaggaacatc gctcctagtc ggcggtggca ggtcgagagg tccgatcagc gtccagggcg
     1381 gcgcgtacgg tgatcctcaa caggaaacgt cctaaggata agtgttatgt tggactcgac
     1441 aacaaaaaca acgactacga agcccaccac ctccccgaag atggacgcta cccaggcgct
     1501 ccgtgagacg gtcgaaagcg gctccgcgca agccaaggaa gctttcgaga agatgagcgc
     1561 tgccacggct gaagccactg cttcgatcaa gaacagctat tcgtcggccg ttaaaggagt
     1621 gcaggactac aacaccaagg tcattgaatt cgctaacgcc aataccgagg ccgcattcgc
     1681 ttttgccaaa acactttctg gcgtgaaatc gccttcagat ttcatcgaat tgtcgaccaa
     1741 atattcaagc aagcagctcg aaacgctgac cgagcagacc aaggaactcg cagagctggc
     1801 ccagaaggtc acgctcgcaa ccacggagtc cttcaagacg ggcatccccc aagcattcgg
     1861 ccagtccact tgagctgatt agctccaaac ttcagcgttc ttcgaagccc gggcaacaac
     1921 ccgggcttct tgctgaagac cgtaaggaag tagccttcat cgacccatca cccacccaac
     1981 tgcgtcgcca atttccgaag cgtggtgtgc ttgtacaccg cccaacgttg agctgcgcgc
     2041 tggcgccgac acggaaaccg ccgagctgat ggcacgattg gcgatgctga cgccgcagca
     2101 ggtgcgcgtg ctgatgatgc tgtcggaggg cctgttcaac aagcagattg ccttccagtt
     2161 aggcgtgtcg gaggcgacgg tgaaggcgca cgtcgccgcc atcttgcaga cgggatcatg
     2221 ccttggcgtt tattcacagg atgtaaaatc tattgggcgg caatccaccg agcaatcggc
     2281 ggccaatgct ctttcagggt acgcgctccc atgaatagcc cgatatggcc gcccggtacg
     2341 gtcttctgca caattcgatc ctgtggtgtc ccaagatatt tggcggcatc aaggacttgc
     2401 tccggtgtcg tgatgtcatc gtctgcaccc gccagcaaat aggcggggca agtgatatcg
     2461 tggagactaa gctgccggcc gagcccgatg aatttgccct tggcaagccg gttctccttg
     2521 aagatttggg tgatcacctg aagataccag cgtcccggca gatcaatcgg attttcgtac
     2581 cagctctcga acgtctcctc cttggccaga taagccgggt cgtcgatgtg ctcgtagaga
     2641 tcgacatggt cctgaatata gtgctgctca gggtgcatgt ttttccagcc ttgcaacatg
     2701 tacttaccct tcgtcagacc accgccgaga gccaccaatt cctcgtagaa cgatgcaggg
     2761 gattcgtgaa ccatgcgttt gataggcccg ttgccggcat cggtgtcgat gggtgcgccc
     2821 gcgagcacca gtgaatttac tttgtcgggg aatcgcgccg ccaccatggc cgccacccaa
     2881 ccgccctggc aaaggccgac cagattcacg cgtccaccga gatcatcgat cgcaataatc
     2941 acttcggcaa ggtagttgtc gatctcaaga tccttcatgt cttcggtcgc cgacttccag
     3001 tcggtcaagg caacgtggcc aattccattg gccagcagag tctcgaccag gctctggcct
     3061 ttatgatagt cggcgatcat cgcggtgtga ccggcatggg gcgcatcgac gagcgtggga
     3121 agtccgcccg gcttgccata atcgcgcagc accatggtcc gcagatcaag gcgtacttgg
     3181 ttcggcgtcg cgagggtggg tctcagatcg tcatgaattt tgatttcttc ctcagcaaat
     3241 ttgaggttct tcgcgtaaag ctccgcccct tcctcgacca acttggctgc catcgccatc
     3301 ggccagaacg ccggcacgct cattaacgac agatcggcgg acttaacgga taactttttt
     3361 gacatgactg atctttgtgc tcatagcgga atgaacgaaa acagaccttg cgaatgtcga
     3421 aagcgtcatt ttctttcgga cattgattta gatgacaatg aggccggcag ggtcggaagg
     3481 cggtgacatg aacgcaagtg tcatcgcttg atcgtttctg caccaagcgg cctgatctcc
     3541 ggcgctccgt ccaacgtgga cgttgctgtc ttttgtcgtc tcatgtggtt ctcgcttctc
     3601 tgtgtccgga tctcatatcg cgttatcgat tccttaccat cccatcgttc ttagtcaggc
     3661 gccatgtatg gcgcgcaatc atcagatctt cgtcggtcgg aatgacccac gcggaggctc
     3721 tgctgtcggc tatcgtgatt ttggggccgc cgacggcgtt cgccgcttca tcgaagtgca
     3781 caccgagcca
//
LOCUS       DASRKK010000158         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_10375, whole genome shotgun sequence.
ACCESSION   DASRKK010000158 DASRKK010000000
VERSION     DASRKK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10375"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..863
                     /locus_tag="VFC15_03535"
     CDS             276..863
                     /locus_tag="VFC15_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09266.1"
                     /translation="MTGIEARLAASLLALTFFLFVVLMHAPAWAHDPRNRFGLTFVLR
                     EISFSGGALALAASLSEQWRERGEHILATIARYFLAIPVLFFSFEQFLHGDHVPGIPL
                     EPLTPQWIYGHAIWTYLAAVVYAVAGTLLLVGKKTRAAAAWLGLTVLFVELVVYVPIG
                     VVERASLDNGLNYVADTLMFCGAVLMLAGAMPREA"
     gene            complement(1055..1732)
                     /locus_tag="VFC15_03540"
     CDS             complement(1055..1732)
                     /locus_tag="VFC15_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM09267.1"
                     /translation="MVGIKAVFVSDHKPVVIEQYNPATKGSHPAVMVVHGGGGPDGDW
                     RKSGLLEALTAAGYSAFVPHYFDGKGGRWDGSDQPDQFVAYIRTLNDASRYIGRQPDV
                     KNNRIGLVGFSLGGYLVLGFAEEVRSHPPPLHSPEIKAVVEMYGGMPGFAVSRMTTMP
                     PLLILHGEDDDVVPVTRAYDVEELLRKKGVSYEIKVYSHQGHGFSGDALKDANERIVF
                     FLRTHLQ"
     gene            complement(1949..2524)
                     /locus_tag="VFC15_03545"
     CDS             complement(1949..2524)
                     /locus_tag="VFC15_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09268.1"
                     /translation="MDDAALAAGKVQIIAPKGFMQESIQENLFGGNAMTRRKSDTYGD
                     ILPKSPYGQVDASIGKAVASGDVGILPPTKIIDEAMEEITIDEVRMVFQNTPGSEAPA
                     EMNTWFPDFKAFWAAENITGILHNILTLRGAPVRDALAWSQYINEALYRFGDLLRFGR
                     RSNDSWYSHFSSSIPALPFCHAELKGAYCYL"
BASE COUNT          596 a          807 c          777 g          623 t
ORIGIN      
        1 ggctggcagg gcacgctcgc tggaaaacgg tacagtgatt tgccagcggc gcgtgggcgg
       61 gttgcatcac cgctatgact gggctgcttg aatcggctgc aggcaagctt tcatttgtct
      121 ggccccacga cagaagtgtc cgctcattca gccggttacg aagaagtccc gcgcgtttgg
      181 ccattcaacg ccacagacaa gttaggtcct gatatcagcg aactcttgag tatgcactgc
      241 tcctggtgcg ttttggattg tgacgaggca cactggtgac gggcattgag gcgcgtctcg
      301 ccgcgagttt gctcgccttg acgtttttcc ttttcgtcgt gctgatgcac gccccagctt
      361 gggcacacga tccgagaaat cggtttgggc tgacgttcgt gctgcgggaa atatccttca
      421 gcggcggcgc tctggcgctg gcagccagcc tcagcgagca gtggcgcgag cgcggcgagc
      481 acatccttgc gacgattgca cgatacttcc ttgcaattcc tgtgctgttt ttcagcttcg
      541 agcaattcct gcacggcgat cacgttcccg gcattccgct ggaaccactg actccccagt
      601 ggatctacgg acacgccatt tggacgtacc tggcggccgt ggtttacgcc gtcgcgggta
      661 ccctgttgct ggttggcaag aaaacccgcg cagcagcggc atggctgggc ttgaccgtgc
      721 tcttcgtgga gttggttgtc tacgtgccaa tcggagtagt ggaacgcgcc agcctcgaca
      781 acggccttaa ttacgtagcc gataccctga tgttttgcgg cgccgttctt atgctcgctg
      841 gcgccatgcc gcgggaagcg tagcggttgg aatcattggc tgatcagtgg ccccacgaaa
      901 cccgcgtcga gcttcccatg cttcagcgag cggaaatcga atcggtttga tttgatgaca
      961 ggaagaacgg atgtgttgca actttcacgt caccggccag ccgccctgaa agttgcaaca
     1021 catccttttc aacagtcaca gcccggagtt ccatctactg aagatgagtt cttaagaaga
     1081 atacaatccg ctcattcgca tctttcaatg catcgccact gaacccgtgt ccctggtgtg
     1141 aatagacctt aatctcataa gagacacctt tctttcttag caactcttcc acatcgtagg
     1201 cgcgggtaac cgggaccaca tcatcgtctt cgccgtgaag aataagcaat ggcggcatgg
     1261 ttgtcattcg cgagacggca aagcccggca tgccaccata catctcgacg acggctttga
     1321 tctcgggaga atgcagcggc gggggatggc ttcttacttc ctccgcaaaa ccaagcacga
     1381 ggtatccgcc gagagaaaac ccaactaagc caattcggtt gttcttgaca tccggctgtc
     1441 gcccgatgta gcgagaggcg tcattcaagg ttcggatgta ggcaacaaac tgatccggct
     1501 ggtccgaacc gtcccatcgg cctcccttgc cgtcaaaata atgcggcaca aaggccgaat
     1561 aacctgcggc agtgagagct tcgagaagtc cactttttcg ccagtctccg tccggcccac
     1621 ctcctccatg cacgaccatc accgctggat gtgaaccctt ggttgcggga ttgtactgct
     1681 cgatcaccac aggtttgtgg tcgctaacaa agacagcctt gatgccgacc aactgggcgc
     1741 caacgggtaa actcgttatg acggcgagaa cagggaatag tctgaaggtg gctcgcaccc
     1801 ggcaactcca atttaggttt atacgaatct aactgcaaag ggcaatgcgt cgcatacaag
     1861 aaacttcaaa gccgctcatt ttcttggctt ctctcagaac cagagcgcgg ccataactca
     1921 gtagcgaata ttaaaagtca caaccctttt acaagtaaca atatgctcct ttaagctcgg
     1981 catggcaaaa aggcaacgca gggatggagg acgagaaatg cgagtaccat gaatcattgc
     2041 tgcgtctccc gaatctgagc agatccccga agcggtacag ggcctcgttg atgtactgtg
     2101 accaggcgag cgcatcgcgc accggtgcac cgcgcagcgt gaggatgtta tgcaggatgc
     2161 cggtgatgtt ctccgcagcc cagaacgctt tgaaatcggg aaaccaggtg ttcatctcgg
     2221 cgggcgcttc gctgcccggc gtattctgga acaccatcct gacttcgtcg atggttatct
     2281 cctccatcgc ctcatcgatg atcttggtcg gcggcagaat gcccacatcg ccgctggcga
     2341 cagccttacc aatcgatgca tcgacctggc cgtacggact cttcggcaag atgtcgccat
     2401 aggtgtccga cttgcggcgg gtcatggcgt tgccgccgaa gaggttctcc tggatggatt
     2461 cctgcatgaa acctttcggc gcaatgatct gtacctttcc agctgcgagt gcggcgtcgt
     2521 ccactactcc acggataccg ccgaaatgat caccatgggc gcgaaagtaa tcctcgggcg
     2581 atccagcgct tcatgtcgcg gcccgaaacc ggcttcggga catgtaccgc agccggatct
     2641 caccaataac gtgatggacc agaagaggac ttctggcccg cgacgacggg actctgctct
     2701 ggcccacaca ttcagagtcg tcgaactagt acgtgctggg cgcttgcagt ccttctagca
     2761 gcgccttgtg gaaacgctcc ggcgcctgga tctgcggggc gtg
//
LOCUS       DASRKK010000159         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1280908, whole genome shotgun sequence.
ACCESSION   DASRKK010000159 DASRKK010000000
VERSION     DASRKK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280908"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..120
                     /gene="rrf"
                     /locus_tag="VFC15_03550"
     rRNA            5..120
                     /gene="rrf"
                     /locus_tag="VFC15_03550"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            263..424
                     /locus_tag="VFC15_03555"
     CDS             263..424
                     /locus_tag="VFC15_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09269.1"
                     /translation="MAARRIPTAVPVENQPAAEWEAMRRRHQLGDEQIVALWEWARAM
                     QDRKTATTT"
     gene            421..660
                     /locus_tag="VFC15_03560"
     CDS             421..660
                     /locus_tag="VFC15_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM09270.1"
                     /translation="MNFTPASLARITASTLIVYGDRDPLYPVEMGVDMYRAIPRSALW
                     IVPNGGHGSIFFDAAPQFVQTALSFLRAQGGTPSP"
     gene            915..1715
                     /locus_tag="VFC15_03565"
     CDS             915..1715
                     /locus_tag="VFC15_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HZM09271.1"
                     /translation="MKRTKSPLHLSRSPLVVVVTEARFSAVMAIEKFVPDIQEQLRHK
                     GFPRFVKNQIQEILVQQNAAPKVNVTDRFEFQNKEQSVGIVLTSNSVAVHTSRYTSFE
                     DFEGIFATVLIVVNRVLQLSLLERVGLRYIDVVRVNQNAGEKLTDYLKPGLLGLDPEK
                     IRAKDWIGRLEQSGRTECGKLVVKCLQHNQILPPDLSPSSLSYGMTLQQGEIVTLLDF
                     DHFADKSDEFETKYVVAAIGELHDALDLAFREAVTDEALIRWGKEESR"
     gene            1712..2368
                     /locus_tag="VFC15_03570"
     CDS             1712..2368
                     /locus_tag="VFC15_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM09272.1"
                     /translation="MNEERYSVVDVSDQSRVPAPSHVVRWNNPLLVTLVGLMVGTGGV
                     ANAGSLRSVSPSSDLKFTVCTDQQQKREPEEDFLIQGKLAAIQQYLALNVTQLSQALG
                     VTRPTVYAWLKAKADPQPIHLARIQRIYSVARRWRAMSSFPMGQLLTTVVPSSGKSLM
                     DVLIGDTLSETETNLVLAQLKLVGESVPRRKSIADVARSHSLQPVQVGESLSRDELDL
                     "
     gene            2397..3032
                     /locus_tag="VFC15_03575"
     CDS             2397..3032
                     /locus_tag="VFC15_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09273.1"
                     /translation="MANGSPRRQSPWRQGSVIPLELLDSGALPNDLDPDAKLIVISQD
                     CDVVHPSYNVEPFVEYFIARRITSRDGRRTRGKNPRFLQFEALINGDGQLFEINANEK
                     FRIDRWILDRGDPEAGVMITPGQIRALARWAAKRYNRSSFPTGFDQRISARVREKIEK
                     LMTRYGGDISGTFIAFLSSDGELREEDAYKILVHVVAQKEAFDGGVREADS"
     gene            3046..3207
                     /locus_tag="VFC15_03580"
     CDS             3046..3207
                     /locus_tag="VFC15_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09274.1"
                     /translation="MTTLLGQCSGIELVETVELDSESEFSLDDYFNTRLWDYEYVSDA
                     EQDEHGGLP"
     gene            complement(4160..4573)
                     /locus_tag="VFC15_03585"
     CDS             complement(4160..4573)
                     /locus_tag="VFC15_03585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZM09275.1"
                     /translation="MPDAIAQFAKAKYLNLETFRKTGVGVRTPVWFAQDAGAPSRADF
                     SPDGGSVFYIYSTPDAGKVKRIRNNARVRVAPCDIRGNLRGAWVDAHARICTGPEAAH
                     GQDLLRRKYGWMKIAGDFFSRLRGRTQVVIAIHID"
     gene            4711..5115
                     /locus_tag="VFC15_03590"
     CDS             4711..5115
                     /locus_tag="VFC15_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZM09276.1"
                     /translation="MVLAKKTICEPGRERNEMRLLLNWLLSAISLLIVSKVVPGFEVH
                     GFKAALIAALVIGLVNATLGLFLKLITLPLTFLTLGIFWWVINALMLMFASTLLAPDF
                     MVRGFWPAFWGAIVLALINMVLKSLTREAERG"
BASE COUNT         1197 a         1448 c         1592 g         1233 t
ORIGIN      
        1 agttgccggt gatcttaccg cggggaaaca cccgttccca tcccgaacac ggaagttaag
       61 ccccgcaggg ccgattgtac tgcacgcgaa agtgtgtggg agagtaggtc gtcgccgaca
      121 taaataaagg ctccatgtga ataacatgga gccttttccg ttttgctctt cttttgtgct
      181 gtcatcccga gcggagcgga gggatctgcg gttgtcatcc tgagcgcagg cggcgcttcg
      241 ccgccggagt cgaaggaccc ctatggcggc gcgtagaatt ccgaccgcag tgccagtgga
      301 gaaccagcca gcggcggagt gggaggcgat gcgacgccgc caccaactgg gcgacgagca
      361 gatcgtcgct ctgtgggagt gggcgcgcgc catgcaagac agaaagacag ctacgacgac
      421 atgaacttca cgcccgcgtc actcgcccgg atcaccgcct ctaccctgat cgtttacggt
      481 gaccgcgatc ctctctatcc ggtggagatg ggtgttgaca tgtaccgcgc tattcctcgc
      541 tccgcgcttt ggattgtgcc caatggcggt catggatcaa tcttcttcga cgccgcgccg
      601 cagttcgtcc agaccgcact gtcgttcttg agggcgcagg gcggaacgcc aagcccgtag
      661 aaccggtgcc atacctgcga cggcatcctg gcggcacgcg aaattgaggg aaagtaggtc
      721 gtcgccgaca taaatgccga cataagtaaa ggcctcacgt gaacaacgtg gggccgtttc
      781 cgtttgccga gaaggccacc tcggtgcgct cggaacgccg ctttccgccg ccggaaatga
      841 caaaatctgc cagaatagga cctacccatc gagatttttc ttgtaaagtg taaactttgg
      901 tgtaaacttt ttatgtgaag cgtacaaagt cgcctctgca tcttagccga tctcccttgg
      961 tagtcgttgt gaccgaggcg cgtttctcag ccgtgatggc catagaaaag ttcgttcctg
     1021 atatccagga gcaacttcgc cataagggat ttcctcggtt tgtcaaaaat cagatacagg
     1081 aaatattggt ccaacagaat gcggctccta aagtcaacgt caccgatcgt ttcgaattcc
     1141 agaacaaaga gcagtcagtt gggattgttc tgacttcaaa ttccgtcgcc gttcatacga
     1201 gccgatacac aagttttgag gattttgaag ggatattcgc cactgtgttg atagtggtga
     1261 atagagttct ccaactcagc ctgttggaaa gagttgggct gcggtatatc gacgtggtca
     1321 gagtcaacca gaatgctggg gagaaactca cggattatct caagccggga cttttgggac
     1381 tcgatcctga aaaaatcaga gccaaagact ggataggtcg tttggagcag tcaggaagaa
     1441 ccgagtgcgg aaagctcgtg gtcaagtgtt tacaacacaa tcaaatactc ccgccggatc
     1501 tatctccgag ttccctaagt tatggaatga cgttacaaca gggcgagatc gtcacattgc
     1561 tcgacttcga ccatttcgcg gataagtcgg atgaattcga gacgaagtat gttgtcgcgg
     1621 caatagggga actccacgat gcgttggatc tcgccttccg ggaagcagta acggatgaag
     1681 cgctgatccg ctggggaaag gaggagtcca gatgaacgaa gaacgctatt cagtggtaga
     1741 cgtttcggac caatcccgag tccctgcacc atcccacgtg gtgcggtgga acaatcctct
     1801 tttggtaaca ctggtcggac tgatggtagg aaccgggggg gttgcaaatg cgggctcgtt
     1861 gcgatcggtt agtccaagta gcgatctgaa attcacggtt tgcaccgatc agcaacagaa
     1921 gagggagcca gaagaggatt ttctgattca aggaaaatta gctgcgatcc agcagtatct
     1981 tgcgctaaat gttactcaac tctcacaagc actcggtgtg acgcggccga ctgtctacgc
     2041 atggctgaaa gcgaaagcgg atcctcagcc tattcatctt gcgcgcatcc aaagaatcta
     2101 ttcagtcgcc cgtagatggc gtgcaatgtc gagtttccca atggggcaac tgctaacgac
     2161 agtcgtccct agtagcggga agtcactgat ggacgtgctt ataggagata ccctgagcga
     2221 aacagagacg aacttggttc tggcacagct caagttggtt ggggaatcgg taccacgtcg
     2281 aaagagtatt gctgacgtcg cacgcagcca cagcctacag cctgttcaag ttggtgagag
     2341 tttgtcgagg gatgagctcg acctatgact ctcgagaggc tcagcaaggt gttctagtgg
     2401 cgaacggatc accgcggcgt cagtcgcctt ggaggcaggg ttctgtaatc ccgctcgaac
     2461 tgctggattc tggagcccta cccaacgatc ttgatccaga cgcaaaactg attgtgattt
     2521 cgcaagattg cgacgttgtg catccctcgt acaatgtcga accttttgtc gagtatttca
     2581 tcgctcggcg gatcaccagt cgagatggac gacgtacccg agggaagaac cctcgttttc
     2641 tgcagttcga agctctgatc aacggcgatg ggcagctatt tgaaatcaat gccaatgaga
     2701 agttcagaat agaccggtgg atcttggacc gaggtgatcc ggaggcggga gtaatgatta
     2761 cgccggggca gatacgggcc ctcgctcgat gggcagcgaa gcgctataac agatcgtcat
     2821 tcccgaccgg ctttgaccaa cggatttccg cgagggtccg cgaaaagatt gaaaagctga
     2881 tgacgaggta tggaggcgac atttccggta catttatcgc gttcctaagt tcggacggcg
     2941 agttacgcga agaggatgca tacaaaattc tcgtacacgt cgttgcacag aaggaagcgt
     3001 tcgacggcgg cgttcgcgag gcggattctt aaagtcaagg ccgaattgac gacacttctt
     3061 ggtcaatgct ctggaatcga actggtcgag acagtcgaac ttgacagcga atctgaattt
     3121 agccttgacg actatttcaa cacgcgactt tgggactatg agtacgtcag cgatgcggag
     3181 caagacgagc acggaggtct gccgtagtat cagccgggtg ccccaccctt gtccgccgat
     3241 gttgcggaca gggtgggagt cgggaatctt caactgctct tgccgccctg cggagcgggc
     3301 gctgcgcgca gcagcccttc ggtgcggggt gccgtccctg aagggactcg ggtttcatta
     3361 ttgcgcctac ccagcgctga cacgctgggc tatcttcttt cgtctcgcaa ggcgggactg
     3421 cggttcatcg cagttaccgc agaccagcgc gaactcagtt taggatcgca gatccaccta
     3481 gaaacgaaga tccccaccct agccaaagga aggctaggat gggccacccg catccttcag
     3541 tggttacctt cgttctgggg aatgcgtttt ctcctcatcc cattcatccc cgcaacataa
     3601 aatgagaagc gctggagtca gtctccggcg ccgcgaatgc ccgtctaggc tgtgcagccg
     3661 tgatggtgaa tggcttccgg gcagggcagc gatctttgaa aaccagcaaa gcctgcaacc
     3721 ctggggcatc cggtaagtgt ttttttagca atatcttgcg tataattgct ttagatagaa
     3781 tattttggcg tgaatcgcgc aagatattga gcagcaaaga cttacagctg cgcttaagtt
     3841 acgtgttatg aatattttgc gggcaaagta ttgaaaacaa agatgcagaa atgctggggg
     3901 gtacttagcg gcgcgttttt ccgtgttgtg gcctaacgcc gggtgcacca cccttgtccg
     3961 ccgatgatgg gggaagggtg ggagttggaa atcttcaact gctcttgccg ccacgcggag
     4021 cgggcgctcc gcgcagcagc ccctcgatgc tgatgtcttc gtcaatctct ggccagtgga
     4081 taccgtagcc gcctccggca atcttccaat ggtgccggtt gccgactggc cgctagaaag
     4141 tccagcgccg ctcgcgatgt cagtcaatgt gaatcgcgat caccacctgc gtccttcccc
     4201 gcaggcggct gaagaagtct ccggcgatct tcatccagcc atatttgcgg cgcagcaggt
     4261 cctggccgtg cgccgcttcc gggcctgtgc agatgcgggc atgtgcatcc acccatgctc
     4321 cgcgaagatt cccgcgaatg tcgcagggcg cgacccgcac cctggcattg ttgcgaatcc
     4381 gcttgacctt gccggcgtcc ggcgttgaat aaatgtagaa gacgcttcct ccgtcaggcg
     4441 aaaaatcggc gcgcgacggg gcccccgcat cctgcgcgaa ccatactgga gtgcgcacac
     4501 ccacgccggt tttgcggaag gtttcgaggt tcaggtattt ggctttggcg aattgcgcga
     4561 tcgcatcagg catcgtcgac aacctctgcc atgagcttat cgcatcgtgg gtcgggcggg
     4621 actgccggcg cagtcgctct ccctcgcggt ataatttccc gcgagtttcc ctcgtccaaa
     4681 gggacttgct ggtgcaccaa agccgtcagc atggtcctgg cgaagaagac gatctgtgag
     4741 ccgggccgcg aaaggaacga aatgcgtttg ctgctgaact ggttattgag cgcgatcagc
     4801 ctgctgattg tgtcgaaggt ggtgcctgga ttcgaggtgc acggatttaa ggcggcgctg
     4861 atcgccgcgc tggtgatcgg gctggtgaac gccacgctcg ggctcttttt gaaactcatc
     4921 accttgcccc tgactttcct taccctggga atcttctggt gggtcatcaa tgcgctgatg
     4981 ctgatgtttg cctcgacgct gctggctccc gatttcatgg tccgcggatt ctggccggcc
     5041 ttttggggag cgatcgtgtt ggcactgatc aatatggtgc tgaagtcgct gacgcgggaa
     5101 gcggaacgag gctagccgtc gaatttgtgg cgcagatagt tctggggcgc agcgaccttt
     5161 agcgccaccc aatagaaact tggtggacca cggatgaaca cggattctca cggaccttct
     5221 tttgagtgga agctacccgt ggttccgtcc gcgatatcaa cggctttcag cttcgcgccg
     5281 ccggccgagt acatcacatt ttcctattga tccgtgctca tccgtgtcga tccgtggtca
     5341 atttgttttt gagaggttag tgtgttcaag ctcgcgcagg cggcgaaccg tctcgacgat
     5401 gtcgcccatc tgaaacaggc gaattcccgc cactccggcg gcgccagcag cgaggcaggc
     5461 ccccgcgttc
//
LOCUS       DASRKK010000160         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_987809, whole genome shotgun sequence.
ACCESSION   DASRKK010000160 DASRKK010000000
VERSION     DASRKK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987809"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(733..2112)
                     /locus_tag="VFC15_03595"
     CDS             complement(733..2112)
                     /locus_tag="VFC15_03595"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZM09277.1"
                     /translation="MSSAAVSYARENQKRFLDELKDLLRIPSISTAEEHKDDVRKAAE
                     VVANDLKRIGFEHVETIATQGHPLVYADWLHASDKPTALCYAHFDVQPAEPLDEWNTP
                     PFEPTERNQNIYARGAVDDKGQLWMQLKAFESLFKTGNGKLPINVRVIFEGEEEVGGE
                     SIEEFVKKNASSGKLKADFALVTDTELFAPDLPTLCVGLRGLVYTEIEAQGAKSDLHS
                     GVYGGAAPNPLFALIEIISKLKDADGKVLIPGFYDDVQKPTADELKAWDRLPFNEEDY
                     RKAEVGSNVLTGEPGYSVMYRTWARPTLEVHGMPGGFVAPGAKTVIPARASAKVSMRL
                     VPKQDPDDILKKYTEFVKKLTPKGIQINIKVHSKGPACVVNTDNKYARASVEAMHEIF
                     GKDTVYIRSGGSIPIVTQFEEDLKIPSIMMGMGLPDDNLHAPNEKFHIPNFYRGIESI
                     IRFFQILGQ"
     gene            complement(2859..3032)
                     /locus_tag="VFC15_03600"
     CDS             complement(2859..3032)
                     /locus_tag="VFC15_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09278.1"
                     /translation="MKWKDEIVEEVRATREAYAAQFDYDLKRMFEDLKAKEVNNPARR
                     AELKPLQPHVQRT"
     gene            complement(3053..>3466)
                     /locus_tag="VFC15_03605"
     CDS             complement(3053..>3466)
                     /locus_tag="VFC15_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZM09279.1"
                     /translation="IMELLNEMYSYEDSVQRGEPAAPPALVTDAQRKLVLMLAPFAPY
                     LAHELWEMLGETSELLRAPWPQYDSALAKEEEVEIAIQVNGKIRSRITIPADAEEAQV
                     RELALADEKVKAAIAGKEVVKVLVVKGRLVNIAVK"
BASE COUNT          651 a         1042 c          980 g          793 t
ORIGIN      
        1 gatctcaccg caaggagtac tatttaacgc cctcaaccgg actcttggca tgcttacgcg
       61 catgatgcct gggttgttcg gataccagat catgctcata gctcgtcgtg acccggacat
      121 cgctggacgg ttccaccaac aaaacgggcg gctttaagct gcctttagct cacggtcgat
      181 atgttgtcag ttacagcttc gccattcttg atgtattagg aagaacggct ctgtgcagga
      241 atctgaacag atgggcattt ttgctggttg ccgctttccg ggattctgga gccgcattgt
      301 cgttgtcatt ccaatcccca cgttttcttg aagcgatcca cgtcatctgg atgataggca
      361 gtaccctgat cgggatggtg tttgtctcgc catactgcag gtccttcttt gtcattccag
      421 aacagcgttc ggcccttgct tgggtgttgc acatcgttcc cggttgcacc ttggacagtg
      481 tcagaccatg cgcttgtact tatctttcga ctggaagtcg tattgaccgc gctgcgtgcc
      541 ccatcctaac gtcgtgcctt tcgaagttag ggatggggtt gttgatgttg ctcccgttcg
      601 tggaacggat cacggcccgt ggatcgatca gcgatggaca gcgcaaaagt caaaccccca
      661 cccaagcaaa agcagcttga gtggggtacc ctgtttctca ctgactactg gccactgact
      721 actagccact gactactgcc ccagtatctg gaagaagcga atgatcgact cgatcccacg
      781 atagaagttc gggatgtgga acttctcgtt gggggcgtgc aggttgtcgt cgggcagacc
      841 catgcccatc atgatgctgg gaatcttcag gtcttcctcg aactgggtca cgatcggaat
      901 cgaaccgccc gagcgaatgt acacggtgtc ttttccgaag atctcgtgca tggcttccac
      961 cgacgcgcgc gcgtacttgt tgtcggtatt caccacgcag gccgggccct tgctgtgcac
     1021 cttgatgttg atctgaatgc ccttgggcgt cagcttcttc acgaactcgg tgtacttctt
     1081 caggatgtcg tccgggtcct gcttgggcac cagccgcatc gagactttgg ccgacgcgcg
     1141 cgcgggaatc accgtcttcg cgccgggagc gacgaagccg cccggcatgc cgtgcacctc
     1201 cagcgtcggg cgcgcccacg tgcgatacat caccgagtag cccggctcgc cggtcagcac
     1261 gttcgatccc acttcagcct tgcgataatc ctcttcattg aagggcagac ggtcccaggc
     1321 tttcagttcg tcggcggtcg gcttctgcac gtcgtcgtag aagccgggga tgagaacttt
     1381 gccgtccgca tctttcagct tgctgatgat ctcgatcaga gcgaacagcg ggttgggcgc
     1441 cgctccgccg tacacgccgg agtgcagatc gctcttcgct ccctgcgctt cgatctcggt
     1501 gtagaccagg ccgcgcagtc cgacgcacag cgtaggaaga tcgggagcga acagctcggt
     1561 gtcggtgacc agcgcgaaat cggccttcag cttacccgag cttgcgttct tcttcacgaa
     1621 ctcttcaatg ctctcgccgc cgacttcttc ttcgccctca aagatgacgc gcacattgat
     1681 gggcaacttg ccattgccgg tcttgaacag cgactcaaaa gctttgagct gcatccagag
     1741 ctggcccttg tcgtcgacgg cgccgcgcgc gtagatgttc tgattgcgct cggtgggctc
     1801 gaacggcgga gtgttccact cgtccagcgg ctcggcgggc tgcacgtcga agtgcgcgta
     1861 acacagcgcc gtgggcttgt ccgaggcgtg cagccagtcc gcataaacca agggatggcc
     1921 ttgcgtggca atggtctcga catgctcgaa gccgatgcgc ttcagatcgt tggccaccac
     1981 ttcggcagct ttgcggacat cgtctttgtg ctcttcggcg gtgctgatgc tggggatgcg
     2041 aagcaaatct ttcaactcgt cgagaaaccg tttctggttc tcgcgtgcgt aggaaactgc
     2101 tgcggatgac atagggaaaa gttctccttc cagagatgga ccagcttgcg tgctacctgc
     2161 tcgcaaacat tggtgggcaa accccaaagt atatcgcaac cgaggcgggg agtttgtgac
     2221 gcgcatgcgg cgagctgtac ggtcacgctg cgctgagagg gcggtcgcaa ccccgcgctg
     2281 gaatatccag tacgcgagtc ccgcaaggcg ggacgaaaga gcttagccca gtgcttcagc
     2341 tagactatgc atgaggactt cacgctttga aggggcacgg cttcagccgt gccgtaagcg
     2401 ctgtcattat ggtggcttta gccactgagg gacttcggcg ttgagactaa gtgcgagttc
     2461 cctcaggggc tgaagcccaa ctccccacgc acacgtagcg gcacggctgg aagccgtgcc
     2521 ccttcaaagc ttgctgtctt tagacagatg aataatccag gttcagcgct gggttggcgt
     2581 agagaaggat cccgggtcct gtagggacgg cagagacgaa cattgcggtg cgccgacgat
     2641 caccgcaaac cttaattcca cacaactatc ttcggctcat ggcgcacacc cacgcctgca
     2701 acctggtgca ttgcgtattc cgcactcaaa gaccgggcca acctcattcc tgatcctgaa
     2761 agactctggc aatgcgccgg cggaatcgcg cgccagaaac agattctatt gctggatcgt
     2821 actgcggcca cgcgcgcaag cagacgtcgc gaagggcatc aagtgcgctg tacatgtggt
     2881 tgtagcggct tcaactctgc ccgccgtgcc gggttgttga cttccttcgc tttcaagtcc
     2941 tcgaacatcc gtttgaggtc gtaatcgaac tgagcggcgt acgcctctcg ggtcgcacgc
     3001 acttcctcca cgatttcgtc cttccacttc attggtcaga ttccgttccg cattatttca
     3061 ccgcaatatt caccagccga cccttcacca ccagcacctt cacgacctct ttgcctgcga
     3121 tggctgcctt gaccttctcg tcggcgagcg ccaattcgcg cacctgtgcc tcctcggcgt
     3181 cggcggggat ggtgatgcgg ctgcgaatct tgccattcac ctggatggcg atctcgactt
     3241 cctcttcctt cgccagcgcg gaatcgtact gcggccacgg cgcgcgcaag agctcggaag
     3301 tttcgcccag catctcccac aactcgtgcg cgagatacgg cgcgaacggc gccagcatca
     3361 gcaccagctt gcgctgcgca tcggtcacca gcgccggcgg ggccgccggc tcgccgcgct
     3421 gaacgctgtc ttcgtacgag tacatctcgt tcagcaattc catgat
//
LOCUS       DASRKK010000161         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_205709, whole genome shotgun sequence.
ACCESSION   DASRKK010000161 DASRKK010000000
VERSION     DASRKK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205709"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFC15_03610"
     CDS             <1..348
                     /locus_tag="VFC15_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM09280.1"
                     /translation="IVGGIVIMNIMLVSVTERTREIGIRKALGARPRDVLRQFLIESI
                     TMALIGGMLGVAGGVGIAKLVTLAIGMPSAIKLWAIFAGLAVSAGVGIFFGVYPARKA
                     AKLDPISALRFEL"
     gene            498..755
                     /locus_tag="VFC15_03615"
     CDS             498..755
                     /locus_tag="VFC15_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09281.1"
                     /translation="MIQVELQPEVEARLAAQAQAHGMGVQAYVEGLLNQALGVKNASG
                     WRQPTTAEAAVFFETMAAKSEKIPQLPDEAFRRESFYQDHD"
     gene            755..1210
                     /locus_tag="VFC15_03620"
     CDS             755..1210
                     /locus_tag="VFC15_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM09282.1"
                     /translation="MTNGPRYLVDTNILLRLSKEDDPHYAFVQSATQALIESRTYLCY
                     APQNIGEFWNVCTRPASHNGFGLSIEETDARVNAIERIMTLLPDNEQIYWVWRDLVVG
                     NSVRGVQVHDARLAAAMQVHAVSHILTLNQPDFARYTSIKVVHPRDVSV"
     gene            1257..>2044
                     /locus_tag="VFC15_03625"
     CDS             1257..>2044
                     /locus_tag="VFC15_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09283.1"
                     /translation="MRRSDYKEILRMALETIAKNKMRSGLTVLGIVIGVMMVIVISSV
                     VRRLNSNVEGMLKDMGSDQIFAYHFSPFTFGRPTTEMLTRKELTHDDALAMRDLPHVK
                     AATASLRVVMPQTGTGTFAVKYEGRKTKNTILDGDLPATMQTDDLKIVRGRWFTETEN
                     EHRSQVIVMGSDTAEKLFEDQDPVGKEVNIEGELFQVIGVLAQRKAAFGNGSNPEDNI
                     VRMPIATMHKLHPEQKQYWITVKSTSHADMDTTMDEMRSLLRRRR"
BASE COUNT          465 a          558 c          608 g          413 t
ORIGIN      
        1 atcgtcggcg gcatcgtgat catgaacatc atgctggtgt cggtgacgga gcgcacgcgg
       61 gaaatcggca ttcgcaaagc cttgggagcg cggccgcgcg acgtgctgcg gcaattcctg
      121 atcgagtcca tcactatggc gctgataggc ggaatgctcg gcgtagcggg aggcgtcggt
      181 atcgcaaagc ttgtgaccct ggcgatcggg atgccgtcgg cgatcaagct atgggccatc
      241 ttcgccggcc tggcggtctc ggcgggagtg ggaattttct tcggggtgta cccggcacgc
      301 aaggcggcca agttggaccc aatttcggcc ttgcggtttg aactctagag tgcggtgttt
      361 taagagagtg gcgggttgac caaccccacc cgggtcctat tggtgcctgg cctaaaatca
      421 gttgcttcgg ctcgctgaca gtttgtggga ctgaaagagc ttacgtggcg ctgtatcatg
      481 ggcgcaggag aaactgcatg attcaagttg aattgcaacc cgaggttgag gcacgactgg
      541 cagcccaggc ccaggcccat ggcatgggtg tacaagctta tgtcgagggc ctgcttaacc
      601 aggccttggg agttaagaac gcttctggat ggcgccagcc gacaaccgca gaggccgccg
      661 tcttctttga aaccatggcg gccaagtccg aaaagatccc tcaactaccc gatgaagctt
      721 ttcgccgcga gagcttttat caggaccacg attaatgaca aacggcccga gatacctagt
      781 tgatacgaac attcttctcc ggctctcaaa agaagacgac ccacactacg cttttgtgca
      841 gtccgctact caggccctca tagaaagtcg gacgtacctg tgctacgccc cccaaaacat
      901 aggcgagttt tggaacgtgt gtacccgccc cgccagccac aacggctttg gtctgtctat
      961 tgaggaaacc gatgcgcgtg tcaacgcaat tgagcgcatc atgactctgc ttccagacaa
     1021 cgaacaaatc tattgggtct ggcgagactt agttgtgggc aatagtgtcc gcggggtgca
     1081 ggtccatgat gcgcggcttg cggccgccat gcaggtccac gcggtttctc atattcttac
     1141 cttgaaccag ccggatttcg cccgttacac aagcatcaag gttgtacatc ctcgagatgt
     1201 ctcagtgtga atgtcggctg agagggcgac gaagagtcag ggcttaagca tttgttatgc
     1261 ggcgaagcga ttacaaagag atcctgcgca tggcgttgga aacgatcgcc aagaacaaga
     1321 tgcgatcggg gttgacggtg ctgggcatcg tgatcggcgt gatgatggtc atcgtcatct
     1381 cgtcggtggt gcggagattg aactctaatg tcgagggcat gttgaaggac atgggctccg
     1441 accagatctt cgcctatcac ttttcgccgt tcaccttcgg ccggccgacc accgagatgc
     1501 tgacccgcaa agagttgacc cacgacgatg cgttggccat gcgtgacctg cctcatgtga
     1561 aggcggcgac cgccagccta cgcgtcgtaa tgccgcaaac ggggacggga accttcgccg
     1621 tgaaatacga gggacgcaaa accaaaaaca ccatcctcga cggcgacctg cccgctacca
     1681 tgcagactga cgacttgaag atcgttcgcg ggcgctggtt cacggaaacc gaaaatgaac
     1741 atcgcagcca ggtgattgtc atgggcagcg acaccgcgga aaaactcttc gaagaccagg
     1801 acccggtagg caaggaagtc aacatcgaag gcgagttatt ccaggtgatc ggcgtgctgg
     1861 cccagcgcaa agcggccttc ggcaacggtt cgaatcccga agacaacatt gtgcgcatgc
     1921 ccatcgccac aatgcacaaa ttgcatcccg agcaaaaaca gtattggatc acggtgaagt
     1981 ccacgtcgca cgcggacatg gacacaacga tggacgaaat gcgctcgctg ctgcggcgcc
     2041 ggcg
//
LOCUS       DASRKK010000162         4551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_792456, whole genome shotgun sequence.
ACCESSION   DASRKK010000162 DASRKK010000000
VERSION     DASRKK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792456"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFC15_03630"
     CDS             <1..198
                     /locus_tag="VFC15_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09284.1"
                     /translation="ELYIEMRKMAKLHDKTDITVRVHPEVAKALKANNGKLISEMEEL
                     TGKTVLVKSDPLLHQENFDIH"
     gene            complement(1542..1814)
                     /locus_tag="VFC15_03635"
     CDS             complement(1542..1814)
                     /locus_tag="VFC15_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09285.1"
                     /translation="MKKASAARRNDDLRPEYDLARLAGGVRGKYYRQAIAGTNLILIE
                     PDLARVFRDSEAVNRALRLLVEAAAAIAPAARARHRAPKKRLQSAR"
     gene            complement(1783..2073)
                     /locus_tag="VFC15_03640"
     CDS             complement(1783..2073)
                     /locus_tag="VFC15_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM09286.1"
                     /translation="MVYEWNAAKAKANAHKHGVSFEEASTVFLDPFAWTFSDPDHSAA
                     EAREITIGYTVRHRVLFVAHCQRGDRTRIIGARKATRRERKHYEEGIGSETK"
     gene            2144..2668
                     /locus_tag="VFC15_03645"
     CDS             2144..2668
                     /locus_tag="VFC15_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09287.1"
                     /translation="MSKLNVSGLVAIVLWSSLCMGQEVHVTQGKGVVVLSVAAQKPYQ
                     LLSGEGKMPMLSVECAHKGKKVGHLVEFSPGGSLVEENFEVDAKPGQLTFNMTIGGTK
                     QMTAWVPYGDTVTFAYFGKSEPERLKFIQSLLNSGVVSIEFKPFLTGVPTTSVFDLSK
                     LRDEMDKYPECATK"
     gene            complement(2859..3194)
                     /locus_tag="VFC15_03650"
     CDS             complement(2859..3194)
                     /locus_tag="VFC15_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM09288.1"
                     /translation="MSKTTTSGKTPKPPRPMTAEALERAARADRDAQPLTETDLKRMK
                     RTPQAKIIRRALELTQEEFAARYHIPLGTLRDWEQGRAQPDQPTRAYLTLIARDPDHV
                     NQTLNPKAN"
     gene            complement(3160..3444)
                     /locus_tag="VFC15_03655"
     CDS             complement(3160..3444)
                     /locus_tag="VFC15_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM09289.1"
                     /translation="MKLEFEWNAAKAEANLEAHGVSFDLATTVFKDPFAVERLDDRKD
                     YGEHRFVILGMAEGHAVLFVAYTEREERIRIISARRATQYEQDDYFRQNA"
     gene            complement(3543..4004)
                     /locus_tag="VFC15_03660"
     CDS             complement(3543..4004)
                     /locus_tag="VFC15_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZM09290.1"
                     /translation="MASLDPKFHEETVSIHKEHEELVAQLDQMDAALEQIVCYSEIFT
                     DLATANQALSHGKWMAEWLPRHYEHEETTVLATIAKMSPELASFAREMKRQHNEMRVR
                     LENFRDRMEHLSETSDLEDAVNQIKKDGTNLTRMMRLHMAAEEKKFASLEN"
     gene            complement(4144..4359)
                     /locus_tag="VFC15_03665"
     CDS             complement(4144..4359)
                     /locus_tag="VFC15_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09291.1"
                     /translation="MNEHGVGNVIMRCNEDIALDMMKFIAVTTGYGKTGSPEAGFQSG
                     TVSKAEDYAQHLLDLFGQCLAAVQGKK"
BASE COUNT          912 a         1320 c         1262 g         1057 t
ORIGIN      
        1 gagctttaca tcgagatgcg caagatggcg aagttgcacg acaagaccga tatcaccgtg
       61 cgcgtgcatc ccgaagtggc caaggcgctg aaggccaaca atggcaagct gatctctgag
      121 atggaggagt tgaccggcaa gaccgtgctg gtgaagagcg atccgctgct gcaccaggaa
      181 aacttcgata ttcactagag gcagtggcta gtggctaggg gatagaaaag ccgggcgcaa
      241 tgcccggctt tttcgctcac agggcgcccc agctcattcg caagagccac ggctgagtgg
      301 gtatcccttc ctagagatta gcaacttcgg ctttcgtcgg aacccgaccg tagactagtc
      361 tagtcggcaa atcttctgcg caagatcaat tcctttggtg tatgagtgtc ccgtgtgaca
      421 ccagccattt tggccatagc gtgagcctac tagctgacga gcggttctca aagttgggca
      481 gccgcgacgc tggatttccg ctaatagccg caaggcccat gcaccatcgt cgcagttgga
      541 acgtcgagtc ttaaactcct catggaaagc cttgcagcac cgctcaagtg tcaccatgtc
      601 aatcttcggt aacaccttgc gatgatcgaa aacaactgac tgaggcatgt tgctcctttc
      661 agtggccacg ccctgccgag tgactgcctc gtgccccagg tccaccgccc tgttgcaggt
      721 tggcgttcaa aacccggacg cgcgcctgga gcaccacgcg cagttcgcct gtttcaaggc
      781 tattctgatt cgtatatccc agacaccgag acggtagcaa cctgcttgcc tgtgtaataa
      841 tccatgaatc tcaccctgta cggttcacgg tttcaaatga caattcgtac caagtcgggc
      901 ctacgacgac cgtagtccag taattgtctc ctttgattga actgaagaca ttcagctttc
      961 gccacatacg acgaagacca ggacactccc gcactaactc agaatgcaaa ttcctagcaa
     1021 actccgcttt acgagcgttt tctgcttgca cctcttcgtc tcgcaaaggg gaagttggcg
     1081 actctaggga gcctctttgt tcgctgtctc cggttggcga ggattccgga ctccgaacag
     1141 tttcactgtg cccagcagtt gtaggttttg gactttcatg atttgagagg ccccacaaca
     1201 gcacaagtcc aaggacagca atggcaccac caataatcaa acttttcggg cgaattcctg
     1261 atccttcggt tgccgaagct aggggacgtg ccccagtggg tacacgtcct gtccacatgg
     1321 gagtcccgca ggattcgcaa aacgtgctct catttttcta agcagcaccg caattggggc
     1381 aaaacatata ggccttccga ggaaaattct acacctgtgg gcgcgtggcc cagcctttcg
     1441 cctgaattct gaccctgagc gcgggtgccc catccaagcc ggttttgctt ggcggggtgt
     1501 gtctaggtgc gacggccttg ccgattgcgt tgtgcgggtt tctaccgcgc cgattgcaag
     1561 cgcttcttag gggcgcgatg ccgcgcgcga gcagcaggag cgatcgcggc tgcagcttcc
     1621 accaggagtc gcaaggcgcg gtttaccgct tccgaatcgc gaaagacgcg ggcgagatcc
     1681 ggctcaatca gaatcaggtt tgtccccgcg atagcctggc ggtagtactt gccgcggacc
     1741 cctcccgcga gccgggcaag atcgtactcc ggacgcaagt cgtcatttcg tctcgctgcc
     1801 gatgccttct tcatagtgtt tgcgctctct cctggtcgcc tttcgggcac cgatgattcg
     1861 ggtgcggtcc ccgcgttggc aatgcgccac aaagaggacc cgatgccgga cggtgtaccc
     1921 gatcgtgatc tcccgcgcct cagcagcaga gtgatccgga tcagaaaacg tccaggcgaa
     1981 agggtcgagg aataccgtcg atgcctcttc aaaggataca ccgtgcttgt gggcgttggc
     2041 ttttgccttc gcagcattcc actcgtagac caccggctca gtttagctca acctggccgg
     2101 gtggcccacc cgaataactt gagggagtag tatgttgatg agcatgtcca aactgaacgt
     2161 atcagggttg gtcgcgatag ttttgtggtc gtcgctgtgc atggggcaag aggtgcatgt
     2221 cacccagggc aagggcgttg ttgttctgag cgttgcggcg cagaagccct atcaattgct
     2281 ctcgggtgag gggaaaatgc ccatgttgag cgtggagtgc gcgcacaagg gcaaaaaggt
     2341 cggacacctt gtggaatttt cgcctggcgg ttccctggtt gaagaaaatt ttgaagtcga
     2401 cgcgaaacct ggacagctga ctttcaatat gacgataggt gggaccaagc aaatgacggc
     2461 gtgggtcccc tacggcgaca ccgtaacctt tgcctatttc ggcaagagtg aacctgagcg
     2521 cttgaaattc atccagtcgt tgctcaactc tggagtagtt tccatagagt tcaagccctt
     2581 cctcacgggg gtgccaacca ccagcgtttt tgacctaagc aaactccgcg atgaaatgga
     2641 caagtatccc gagtgcgcca cgaaatagtt ctatgccggc aacgccccgc caaatccaag
     2701 aaggtcgcat aacgcgcctt ggtgggcggc ccggccaagc acaccccagt cgtcgaaaag
     2761 gctttagatc tgggtgcccc gttctagccc ggttttggct aaggccccac ccacgcttct
     2821 tttgcttgga tgggtttgtt gcaacacggc ggtcgtgctt agtttgcctt tggattcaat
     2881 gtctggttca catggtcggg atcgcgggca atgagtgtta gatacgcccg cgtaggctga
     2941 tccggttgcg cccgaccctg ttcccaatcc cgtagcgtac ccagaggaat gtgatagcgc
     3001 gccgcaaact cctcttgcgt cagttccaag gcccggcgga tgattttcgc ctgcggcgtt
     3061 cgcttcatcc gcttgaggtc ggtttcggtg agcggctggg cgtcgcggtc cgcgcgcgcc
     3121 gcccgctcaa gggcctcggc ggtcatcggc cggggaggct taggcgtttt gccggaagta
     3181 gtcgtcttgc tcatattgcg tagctctccg agctgaaata atgcgaatgc gctcctcgcg
     3241 ttcggtgtag gctacaaaca gtactgcgtg gccctccgcc atgccgagga tgacaaaacg
     3301 gtgctcgcca tagtctttac gatcatcgag gcgctcgacg gcaaacgggt ctttgaacac
     3361 cgtcgtcgcc aaatcgaagc tcacgccatg ggcctccaag ttggcctcgg ctttggcggc
     3421 gttccattca aattccaatt tcatgatact acgggatacc agtagttctg tggactaatg
     3481 agaggaaaat ttgttgcact ttgatcgtgg gcgccttctt gcgtcgtaac tgacgttcac
     3541 cgtcaatttt ccaggctggc gaactttttc tcttccgctg ccatgtgcag ccgcatcatg
     3601 cgagtgaggt tcgtaccgtc tttctttatc tggttgaccg catcttccag gtcgctggtc
     3661 tcgctcaggt gctccatcct atcgcggaaa ttctccagcc gcacccgcat ttcgttgtgc
     3721 tgccgcttca tctcgcgagc gaacgaggcc agttccggac tcatcttcgc gatggttgcc
     3781 agcaccgtgg tctcttcatg ctcgtagtgc cggggaagcc actccgccat ccacttgccg
     3841 tggctcagcg cctggttggc ggtggcaaga tcggtgaaaa tctccgagta gcagacaatc
     3901 tgttccagag cggcatccat ctggtccaac tgcgccacca gttcctcgtg ttctttatga
     3961 atgctgactg tctcttcgtg gaatttagga tccaagcttg ccatgtcgca cctcgccagc
     4021 ttgatggtcg tcccgcccct gcgcccgcgg cagtgaccga aatcacttac tggcgtgacg
     4081 ttcggcggtc atcccgcaat aaacaagacc ggagctggca gccccggccg gttgtttttg
     4141 cgcttatttc ttgccttgca ccgccgccag gcattgtcca aacagatcca gcaaatgctg
     4201 ggcatagtcc tcggctttcg aaaccgtacc gctctggaac ccggcttccg gcgagccggt
     4261 cttcccgtat ccggtggtga cggcgatgaa tttcatcatg tccagcgcga tgtcttcgtt
     4321 gcaacgcatg atcacgttac cgacgccgtg ttcattcata agtgcccgac ctccctgttc
     4381 gccggcttct cgcgccgacg ggcaagaatc atagcagaga gttgggcttc agacctcgaa
     4441 ccccacgtgg cacactcgac ccacaatgta gtcgtgataa ctcctgccgc gatcgatgcg
     4501 caccagttcc accgagtgct gcgggttctg cggccgcaac accaggtgat c
//
LOCUS       DASRKK010000163         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_303474, whole genome shotgun sequence.
ACCESSION   DASRKK010000163 DASRKK010000000
VERSION     DASRKK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303474"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1739
                     /locus_tag="VFC15_03670"
     CDS             441..1739
                     /locus_tag="VFC15_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZM09292.1"
                     /translation="MTHRERRSYDHRIKAQVAATGDPNLFPELQIPRSTALSWIRRGV
                     GEVVVVEPGIKVENHHLDRIAKLEHRVSMLTAVLRLVLALLRVSGFGLDVNRIPNAGG
                     KQRLLSAVERARKIMPLTAALRVLGLSAGRYHDWVERQEGCALDDRSSCPRSKPQILT
                     HQEVGAIGDMVQSKDYRHMSIRGLALHAQRVGKVFAHPGTWAKLIRERGWGRPRMRLY
                     PPKPKVGFRATAPNETWHVDATIIKLLDGTKAYLHAVIDNYSRRILAWTVAETLNPMN
                     TCYVLQQAAAGLSESETKVYMDSGVENLNKDVDKLLEGGMLERVIAQIDVTFSNSLIE
                     SWWRSLKHQWLFINHLDNVVTLRKLIEFYVAEHNQMMPHSAFDGQTPDEMYFGRGEAV
                     PDELAQRRREARGSRVEQNRNIACSECPRGGAAVGEDVAA"
     gene            2031..>2420
                     /locus_tag="VFC15_03675"
     CDS             2031..>2420
                     /locus_tag="VFC15_03675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09293.1"
                     /translation="MAHEFDGQKYAQASTHQKEWGHVLIDELQLQGRESVLDLGCGDG
                     ALTARIAELLPLGSVVGIDASRGMIDAAITHLRPNLRFIVMDIRELRLSERFDVAFSN
                     ATLHWVHDHQLLYERLGRLLHKGGRIRF"
BASE COUNT          514 a          705 c          710 g          491 t
ORIGIN      
        1 tatttacccc tggccaagag aagcgaacgg ggacagtagt tagaccgctc gtgcccgggg
       61 cagtgcgccc aacgataggc aaacccggtg tgcccgtgct catagtgccg cccgtggcta
      121 caacaccgcc cgtggctgca acacccccgg ttgctttgga accaccggtc gcgttggcac
      181 caccagttgc tttagagccg ccagttgtcg ctgcgccgcc cgtgctcgta ctgccacccg
      241 taagtcgcgc tccaccggtt gccactaagc cacctgtgtt tgcgttcccg cccgaggccc
      301 cgctgccacc gactgcatta aacccagctg cgtcgctcga catttcggag ccggggcggt
      361 gcaattgcac ggcgtgatat cattagggtt tttgtgcaga cgccacggcc gatgctaact
      421 gtcgtagcgt agttggcctg atgacccacc gcgaacgccg ttcctatgac catcggatca
      481 aagcgcaggt cgccgctact ggcgatccca acttgttccc tgagctccag attccgcgct
      541 ccacagccct gagttggatc cggcgcggag tgggcgaggt ggtggtggtc gaacctggaa
      601 tcaaggtcga gaaccaccac ttggaccgca tcgcgaagtt ggagcatcgc gtgtcgatgc
      661 tcaccgcggt gcttcggttg gtgctggctc tgctgcgagt ctccggtttc gggctcgacg
      721 tcaaccgcat cccaaatgcg ggaggaaagc aacgattgct cagtgccgtt gaacgggcac
      781 gaaagatcat gcccttgacg gcagcccttc gcgttcttgg gctgtccgca ggacgatacc
      841 acgattgggt cgaacgccaa gagggatgcg cgctggatga tcgctcaagc tgtcctcggt
      901 ccaaaccaca gatcttgacg caccaggagg tcggagccat cggcgacatg gttcagtcca
      961 aggactatcg gcatatgtcg attcgcggac ttgctctgca tgctcagcgc gtcggcaaag
     1021 tcttcgctca tccaggcact tgggccaagt tgattcggga aagaggctgg ggccggccac
     1081 ggatgcgtct atatccacca aaaccgaagg tagggttccg cgcaacggcg cctaatgaaa
     1141 cttggcatgt cgatgcgacg attatcaagt tgctggatgg cacgaaagcc tatctccacg
     1201 cggtgataga caattactca cgcaggattt tggcctggac cgttgcggag acactgaacc
     1261 caatgaacac gtgctacgtg ctgcagcaag ccgcagcggg tctgagtgaa tcggaaacca
     1321 aggtgtacat ggattccggc gtagagaacc tcaacaagga cgtggacaag ttgctcgaag
     1381 gtggcatgtt ggagcgcgtc atcgcacaga tagatgtcac cttctccaac tcattaatcg
     1441 agtcctggtg gcgttctctc aagcaccagt ggttgttcat caaccacctc gacaacgtgg
     1501 tcacgttacg aaagctgatc gagttctacg tagctgagca caatcagatg atgccgcata
     1561 gtgcattcga cggtcagaca cccgacgaaa tgtacttcgg acgaggcgag gcagttcccg
     1621 atgagcttgc ccagcgacgt cgagaagcga gggggagtcg cgtagaacag aaccgcaata
     1681 ttgcgtgctc cgagtgccca cgaggtggag ctgctgttgg tgaagacgtc gccgcatgaa
     1741 gaataccgcg aacccgacgg agaattgcgg ctggcgcaat tgcatcaccc aaactccgga
     1801 atgtccggcg acgtagctgg gctgtctccg gcaatccgat cgccgaattc gcgaaatgcg
     1861 tgactccata ccagccgggt cgataacgca ctgcctaact cgtgtttgca tctgacaagg
     1921 gcctgccgat acacttcttg ccttcttccg aatctgaagc gacaattcaa accgatgcgc
     1981 ctgggctggc ccttgcagct gaaacaccag gcgttaggca catgaaacac atggcacacg
     2041 aattcgatgg tcagaagtat gcacaagcgt cgacccacca gaaggagtgg ggccacgtct
     2101 tgatcgacga actgcagctg caagggcgcg agtcggtgct ggatctcggt tgcggcgacg
     2161 gcgcactgac tgcacgtatc gccgaactgc ttccgctggg gtctgtcgtg ggcattgatg
     2221 cctcgcgcgg catgatcgac gctgcgatta cacacttgcg tccgaacctg cgcttcatcg
     2281 ttatggatat ccgagaactg cggctgagcg aacgttttga tgttgccttc tccaatgcca
     2341 ctctgcactg ggtccacgac catcaattgt tgtacgaaag gctggggcgc ctgctgcaca
     2401 agggtggccg catccgattc
//
LOCUS       DASRKK010000164         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_499250, whole genome shotgun sequence.
ACCESSION   DASRKK010000164 DASRKK010000000
VERSION     DASRKK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499250"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFC15_03680"
     CDS             <1..694
                     /locus_tag="VFC15_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HZM09294.1"
                     /translation="LIPPDRELSDLARRRLAALKEFSDLGAGFKIAALDLELRGAGNL
                     LGGQQSGHIEAVGFELYTTMLERTVRELKGEVQEETAETQLNLGLNIRIPSDYIPEEN
                     HRLRMYKRVASVETEAQLEDVAGELGDRYGTPPPPVRSLLEYATLKLLSQRIGVAQIE
                     RRREAVSIRFTETASVDPERLARFVAGEAGSQFTPAGVLKFTLKEKQPEQVLARLKSL
                     LEQLAGEQQAVA"
     gene            926..1807
                     /gene="galU"
                     /locus_tag="VFC15_03685"
     CDS             926..1807
                     /gene="galU"
                     /locus_tag="VFC15_03685"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZM09295.1"
                     /translation="MKKKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPIIQYGVE
                     EALASGCDQIIIVTGRGKSAIEDHFDVSYELEKMLEERGKTDLLAIVRQISEMIHIAY
                     VRQKEAMGLGHAVLMARELVGDEPFAVILADDIIDAEVPCLKQMIEVYNETGRSVLAT
                     QVIEGQAISSYGVLDAKPVPGKWNGRLFEVQNLVEKPKAEDAPSNLAIIGRYILTPAI
                     FEMLDSTPLGAGGELQLTDGLKQLLKKETIYGYTFEGKRHDTGDKLGFLKATVEFALK
                     REDLGGDFRAYLKTLEL"
     gene            complement(2056..2562)
                     /gene="purE"
                     /locus_tag="VFC15_03690"
     CDS             complement(2056..2562)
                     /gene="purE"
                     /locus_tag="VFC15_03690"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZM09296.1"
                     /translation="MDNPLVSIVMGSDSDLEIMNEAAKALAEFGIAYEIDITSAHRSP
                     ARTSEYSRGLKARGVKVVIAGAGGAAHLAGVIAAETTLPVIAVPIPSTALLGLDSLLA
                     MVQMPAGIPVAAVAIGKPGATNAGILAAQMIGLYDAEVARKLEGHKRKLAEGVEAKSR
                     KLKEGQKA"
BASE COUNT          592 a          774 c          860 g          542 t
ORIGIN      
        1 gctcattccg cccgaccgcg agctgagcga cttggcgaga aggaggctgg cggcgctcaa
       61 ggagttcagc gatctgggcg ccggattcaa gattgcggcg ctcgatctgg aattgcgagg
      121 cgccggaaat ctgctgggcg ggcaacagag cggtcacatt gaagccgtcg gtttcgagct
      181 ctacaccacc atgctggagc gcacagtgcg cgaactgaag ggcgaggtgc aggaggagac
      241 ggccgaaacc cagttgaact tggggctgaa catccgtatt ccttccgact acatcccaga
      301 agagaaccat cggctgcgta tgtacaagcg cgtcgccagc gtggagacgg aggcgcaact
      361 ggaggatgtc gcaggcgagt tgggcgatcg ttacggcacg ccgcctccgc cggttcgaag
      421 cctgctggag tacgcaactt tgaaattgct gtcgcagcga atcggcgtcg cgcagattga
      481 gcgccgccgc gaagccgtga gcattcgctt taccgagact gcctcggtcg atccggaacg
      541 cttggcgcgt tttgtagccg gagaagccgg aagccagttc accccggcgg gcgtgctgaa
      601 gtttacgctg aaagagaagc agccggaaca ggtgctggcg cgactgaagt cgttgctcga
      661 acaattggcg ggagagcagc aggcggtcgc atgataacaa cgtatctcat aaactgcttt
      721 gtatcagggc acggcttcca gccgtgccgc aatagggtca ataatgactt gggctttagc
      781 ccctgcggat cctattgtgc aggggctaaa gcccggaact tggcagcact tggttcggca
      841 cgactaaaag tcgtgccctg atacaaaccc agaccgattt atgcgatgag ttcaaattag
      901 cttttcattt catccaagag gacccatgaa gaagaaggtt cgtaaggcag tattcccggc
      961 agcggggcta ggtacgcggt ttctgcctgc taccaaggcc caacccaagg aaatgcttcc
     1021 actggtggac aagcccatca ttcagtacgg cgtcgaagag gcactggcgt cgggttgcga
     1081 ccagatcatc atcgtgactg gccgcggcaa gagcgcgatc gaagaccact tcgacgtcag
     1141 ttacgagctg gaaaagatgc tggaagagcg cgggaagacc gatctgctgg ccattgtgcg
     1201 ccagatttcg gagatgatcc acatcgccta cgtccggcag aaagaagcca tgggcctggg
     1261 tcacgcggtc ttgatggcac gcgagctggt cggagatgaa cccttcgccg tgatcctggc
     1321 cgacgacatc atcgacgctg aagttccgtg cctgaagcag atgatcgagg tctacaacga
     1381 gacgggccgt tccgtgctgg cgacacaggt catcgaaggc caagccatct cttcgtacgg
     1441 cgtgctcgat gccaaaccgg tcccgggcaa atggaatggc cggctctttg aagttcaaaa
     1501 cctggtggag aagccgaaag cggaagatgc accctcgaat ctggcaatca ttgggcggta
     1561 cattctgacg cccgcaatct tcgagatgct ggattcaact ccgctgggcg cgggcggcga
     1621 gttgcagctc accgacggcc tgaagcaact gctcaagaag gaaacaattt atgggtacac
     1681 cttcgaaggc aagcgacatg ataccggcga caagctgggc ttcctgaagg cgacagtaga
     1741 gtttgcgttg aagcgcgagg acctgggcgg cgacttccgg gcgtatctga agacgctgga
     1801 gctgtaaccc tctcaactgt catcctgagt gttacatgga tctgcggccc acccataagg
     1861 gatgaaaatc aaccacggag aaaactcgag agaaaaccaa gactccgtgt cctccgtgcc
     1921 ctctgtggtg aatatttttc gggggagcgg agcgcccacc cagcctttcc gacccgccgc
     1981 ttgtgcggag tcggggcggg cacgcagtcg aaggatctgc ggttcaaggg ccggcaaagc
     2041 ggcgattcag cgcgctcagg ctttttggcc ctccttcagc ttgcgcgatt tcgcttccac
     2101 gccttcggct aatttccgct tatggccttc gagttttctg gccacttctg catcgtaaag
     2161 tccgatcatc tgcgccgcga gaatgccggc gttggttgcg cccggtttgc cgatggcgac
     2221 ggcggcgacg ggaattcccg ctggcatctg caccatcgcc agaagggaat ccaggcccag
     2281 cagcgcggtc gaagggatcg gcaccgcaat cacgggcagc gtagtttcgg cggcgatgac
     2341 gcccgcgaga tgcgctgccc cgcctgcgcc agcgatgacc accttcacgc cgcgcgcttt
     2401 cagcccgcgg gaatactcgc tggtgcgagc aggcgagcgg tgcgccgagg taatgtcgat
     2461 ttcatatgca atgccgaact cggcgagcgc cttcgcggcc tcgttcatga tctcgaggtc
     2521 ggagtcgctg cccatgacga tggagacgag aggattgtcc atgttcgaga ctttctgtga
     2581 tgaacctttc tgtcgtccct gcgggactcg gatttgatct gacgcttacc caccgctgaa
     2641 cctagtacta cagttgtaga tagaatgcgg gcagaggcga tccgcgcttt cgatagtggc
     2701 agcacatagc tcggaattga tggcgtcttc gccactcgga ccaatgcagc aggtgctcaa
     2761 tgccgtgc
//
LOCUS       DASRKK010000165         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1183549, whole genome shotgun sequence.
ACCESSION   DASRKK010000165 DASRKK010000000
VERSION     DASRKK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183549"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1727)
                     /gene="ltrA"
                     /locus_tag="VFC15_03695"
     CDS             complement(429..1727)
                     /gene="ltrA"
                     /locus_tag="VFC15_03695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM09297.1"
                     /translation="MSTQLSQITKKAKLDHKARFTSLAHLLTPEFLKETWGKMNRRAA
                     GGVDGKSAEQFGSELETQVEAICTQLKEGTYHAPPVRRVEIPKGPGKVGTRPLGIPTA
                     ADRLLQRAVARILEAVFEADFLDCSYGFRPGRNPHHALQALRVQIVTKKVGQVFEADI
                     RSYFTRINHQWLRKMVAHRIADPVILSLIGKWLNAGAMQDGIVIYAEEGTPQGGPISP
                     VLSNLYLHFVLDLWFEKKIKPQCRGEAYLLRFADDFVVSFQFREDADHFQQNVRERFA
                     EFGLELAEEKTRRILFGRFAGVTCLRHGLRRPETFEFLGFQHVCGVDRAGRFALIRIP
                     SVKSCRKFLARTREWILHNRHWRRWEQQEHLTKMLRGFYQYFGLHHCERKLNWVRQQV
                     QRQWISALNRRGQRRRTNWTRLKDRLWFKLPFAQNLHPLV"
     gene            complement(2250..2389)
                     /locus_tag="VFC15_03700"
                     /pseudo
     CDS             complement(2250..2389)
                     /locus_tag="VFC15_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          454 a          755 c          611 g          569 t
ORIGIN      
        1 gttatgggat acggattctg agggcaaggt caccgagcag cttccggtgg tttgaaccca
       61 catcctgtaa tgcgagttcg agaggcctac tctcatctct cgtgcagcaa agcttgaaga
      121 agaagtttct cgcttacatt aaccctcctt cgcgccttcg tggcgcactg tcgtccgcat
      181 agcgtacgac aatcacctcg ccttgcgccc ctttctggcg ccaaacgttt acccacaggt
      241 cgaaggggat ggtagagacg agtcttgacg acggtagccc gtgcggagcc taacgaagcg
      301 cagcgctttc gcgagcacct ctcaccgagt gggcatggtc acttgtcctc gtctccccaa
      361 gcagaacccg gcgtgcagat ttcccgcacc gggctcccca gatgacattg gcgaaagtaa
      421 gcatcggttc agaccagcgg gtgcaagttt tgagcaaacg gcagcttgaa ccacaggcga
      481 tccttcaggc gcgtccagtt cgtccgacgt ctctgacccc ggcgattcaa ggcgcttatc
      541 cactggcgct gtacctgctg ccgcacccag ttgagcttgc gctcgcagtg atgcaggccg
      601 aagtactgat agaagcctcg caacatcttc gtcagatgtt cctgttgctc ccatcgtcgc
      661 cagtgtcgat tgtgaaggat ccattcccgt gtcctggcca ggaacttccg acaactctta
      721 acgctaggga tacgaatcag agcaaatcgc cccgctcggt ctaccccaca gacatgttgg
      781 aagcctagaa actcgaaggt ctccggccgt ctcagtccgt gccgcaggca ggtcacgcct
      841 gcgaatcgcc caaagagtat gcgccgagtt ttctcctcgg ccagctccag cccgaattcc
      901 gcgaaccgct cccgcacgtt ttgttggaag tggtccgcat cctctcggaa ctggaaactc
      961 actacgaagt catcagcgaa acgcaacaga tacgcttcgc cccgacattg cggtttgatt
     1021 ttcttctcaa accacaaatc gagaacaaag tggagataaa ggttgctcaa cacgggggag
     1081 atcggaccgc cctgcggggt accttcctct gcgtagatga caatcccatc ttgcatcgct
     1141 ccggcattga gccattttcc gatcaggctc aggattaccg gatctgcaat gcggtgggct
     1201 accatcttgc gtaaccactg atgattaatg cgagtgaaat agctgcggat gtcagcttcg
     1261 aacacctgcc ctaccttctt cgtcacgatc tgcacgcgta gagcttgcag cgcgtggtga
     1321 ggatttcggc caggtcgaaa gccgtagctg caatccagaa aatctgcttc gaacacggct
     1381 tctagaatgc gcgctaccgc tcgctgcagc agtcggtccg cggccgttgg aattcccagc
     1441 ggtcttgtcc cgactttgcc cggccctttg ggtatctcaa ctcttcggac cggcggtgcg
     1501 tggtaggtgc cttcctttag ctgtgtgcag atcgcctcga cttgcgtttc tagctcgctt
     1561 ccaaactgct cggcgctctt tccatcgacc ccgcctgccg ctcgccggtt catctttccc
     1621 caagtctcct tgagaaattc cggcgtcagc aggtgcgcca atgaagtaaa ccgcgccttg
     1681 tggtccaact tcgccttctt cgttatctga ctcagttgtg ttgacatcag attcctgcct
     1741 ctgtgggtgc aagtgatgtt tccctaaagt cagtcttcat ctgcttcgcc gcttcccctt
     1801 gtgcgcggct ttcccgcgct ccgagtacta ccagcgaatc cgacttcgca ctgtggcatc
     1861 tgcctttctc aggaatgatc cttatcgtcc ggcatactcg atccgcttgg taggcgaacc
     1921 aagaccacag tggatctccc aggtcccttg atgcttcctt atccgaacgt gccgtgctcc
     1981 ctgaccccgc cgcagtctcc ggccacctcg ccatttgcgg tgtgcctact ggtgcctccc
     2041 agccactacg atgctgtcgg cctgcggtta agtacttaac gaggctcaat cgcttcactt
     2101 tcgttacggc tcgctcgtcg ctctgtctac gcttagtctg attcgttacc tcctcagacc
     2161 caagactcga ttcccggtaa ggcggctgca cctcttgtcg ggacgggaat ttcacccgct
     2221 ggaaacaccc ggttttgtcc tggcgcaccg tagtgcaagt agacattggc cagcaggggc
     2281 gacgctgtcg cgccctgagg cgtacccgcc tcgctttccg tgacgatccc ttcttcgaat
     2341 actcccgcct taagccattt gcggatcaag cggagtatgc gctcgtccc
//
LOCUS       DASRKK010000166         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_108389, whole genome shotgun sequence.
ACCESSION   DASRKK010000166 DASRKK010000000
VERSION     DASRKK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108389"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFC15_03705"
     CDS             complement(<1..392)
                     /locus_tag="VFC15_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM09298.1"
                     /translation="MAELPFEPATLSPQDKAIYDAMMARRKSHGAPFGGPYAALMNHP
                     QLCQKIEELGYYLKFEGHLARDVYQFVVLSVARETRTAFEWVDHVQHALAAGVPDTVI
                     EALRAHGVTGADYPDPYRLAALVLRAALA"
     gene            complement(412..>2098)
                     /locus_tag="VFC15_03710"
     CDS             complement(412..>2098)
                     /locus_tag="VFC15_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZM09299.1"
                     /translation="REGIDHIFMVPGGLVDPFLPALGRQSVLKPIVAAQEGGAAYMAD
                     GYARASGKFGAALCIGGPGLANTVTAVATALTDGSPLLVISGEAATALEGLGMFQDAS
                     SQTLDDVGMLRPVTCYSTSVDNPKNLAHLFRHALIHLRKEPNGPVHLSVPNDSQVATI
                     DVCHEPLDRALIHARVLSQEAAEVTLGHFKAGIEGTPPVRIAVLAGAGVEHAKAARDL
                     LKFAERWDIPVATTLRAKGIFPEDHPLSLGVFGYSGTHHARMAILDNPPDLFLVLGSG
                     LNERDTMYWTLRLSPTRALCVNLNTAAMATHIPASAVVGDAGAYLQWLEGRTDQIGTA
                     LDATRVARKEWLAGIVAQPRLQGPENCDSSAIPIHPARVISGLRKALPRDGILLVDSG
                     AHRAFAGHYWQAYSPLTYISATNLGPMGWAIPAAIGVQCARPGKKVAVITGDGCMHMH
                     GIEVATAARYRLPIIYVVINNDALGNVWLRAHTLGPVPTELTTLPDIDWAGFSRALGG
                     NGITVTDPSELEPAFETAMCNHGPTVIDVKAGRQYPTPVHDWSVASAAWSYHE"
BASE COUNT          426 a          638 c          616 g          418 t
ORIGIN      
        1 gccagcgccg ctcgcaacac cagcgcggca agccggtagg gatctgggta gtcagcaccg
       61 gtaacgccgt gcgctctcag cgcttcaatt accgtgtctg gaactcccgc agccagggca
      121 tgttggacgt gatccaccca ctcgaacgcc gtcctggttt cacgcgctac cgacagcacc
      181 acaaactgat agacgtctcg cgcaagatgc ccttcaaact ttaggtaata acccagctct
      241 tcgatcttct ggcaaagttg cgggtggttc attaacgcgg cataggggcc gccgaaggga
      301 gcaccatggc tctttcgccg ggccatcatc gcatcgtaaa ttgccttgtc ctggggacta
      361 agcgtcgcag gctcaaaggg taattcggcc actcatcgct cccgtcgatg tttattcgtg
      421 ataagaccaa gccgcggagg ctactgacca gtcgtgcacc ggagttggat attgcctgcc
      481 ggctttgaca tcgatcacgg ttggcccatg attgcacatt gcagtctcga aggctggttc
      541 aagctcgctc gggtcggtaa ccgtgatccc gtttccgccg agtgccctgg aaaaacctgc
      601 ccagtcaatg tcgggtaatg tcgtcagctc agtgggtact ggacccaggg tgtgtgcgcg
      661 taaccagacg ttgcccagcg catcgttgtt aatcactaca taaatgatag gcaaccggta
      721 gcgggcagcg gtcgccactt caatcccatg catgtgcata cagccatcgc cggtgatgac
      781 cgcaaccttt ttgcccgggc gggcacactg cacgccgatc gcggccggaa tggcccatcc
      841 cataggcccc aggttggtgg ccgagatgta ggtcaacgga gagtaggcct gccagtagtg
      901 acctgcaaac gctcgatgcg cacccgaatc gaccagcaga attccgtccc gcggaagtgc
      961 cttgcgtagt ccgctgatga cccgtgccgg gtgaatcgga atcgctgaac tgtcgcagtt
     1021 ctcagggccc tgcaaacgcg gctgggcaac aatacccgcc agccactctt tgcgagcaac
     1081 tcgcgtggca tccagggctg tgccgatctg atcggtccgg ccctctaacc actggagata
     1141 tgcccccgca tcaccgacaa cggcgcttgc ggggatgtgg gttgccatcg ccgcggtatt
     1201 gaggttgaca cacagcgcgc gtgtcggtga aaggcgtaag gtccagtaca tcgtgtcacg
     1261 ttcgttgagg ccggagccca gcaccaggaa caaatccggc ggattgtcga gtatggccat
     1321 gcgagcatga tgagtgccgg agtagccgaa taccccgagt gacaggggat ggtcctcggg
     1381 aaaaatcccc ttggcgcgca gcgtagtcgc aaccggaatg tcccagcgtt cggcaaactt
     1441 caacaggtct cttgcggcct tggcgtgttc caccccagcc cctgccagga cggcaatacg
     1501 caccggcggc gtgccttcta tcccggcttt gaaatggccg agagttactt ctgccgcctc
     1561 ctgcgacagc acgcgcgcat gaatgagtgc gcggtccagc ggctcatggc agacatcgat
     1621 ggtcgcaacc tggctgtcgt tgggaacgga caaatgcacc ggtccgttgg gttccttccg
     1681 cagatgaatc agcgcatggc gaaacaggtg cgccagattt ttcgggttat cgactgaggt
     1741 tgagtagcat gtgaccggcc tgagcatacc gacatcgtcc agagtttggc tgctggcgtc
     1801 ctgaaacata cccaaacctt cgagcgccgt cgccgcttct ccgctgatca ccaatagcgg
     1861 agagccatcg gtcagtgccg tggctacagc ggtcacggtg ttcgcgaggc ctggtccacc
     1921 gatgcacaac gcagccccga acttgccact ggctcgcgcg tagccgtcgg ccatgtaggc
     1981 tgcgccgcct tcttgtgccg caactatggg ctttagaacg ctctgtcgcc ccaatgcggg
     2041 caggaaagga tcaaccaatc caccggggac catgaagata tggtcgatac cttcacga
//
LOCUS       DASRKK010000167         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_303871, whole genome shotgun sequence.
ACCESSION   DASRKK010000167 DASRKK010000000
VERSION     DASRKK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303871"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFC15_03715"
     CDS             <1..1131
                     /locus_tag="VFC15_03715"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZM09300.1"
                     /translation="DPTSGNLDRIQVIKGWTKNGQSFEKIFDVAWSGDRKPNKWSGRV
                     PAIESTVDLEKATYTNTVGSTELKTVWTDPEFDASLHAFYYARVLEIPTPRWTLIQSV
                     KAGLTPPDVVPLTGQERAWSSPIWYTPSADARKNAPAGMTVTDLKAKSATQLGDAQLK
                     ALIVGKAFWVRNNATGEQFSVAYTAEGNSNIWHIGHSATTPSYVGDPVRHGYEGVTTP
                     YKIEGGKVVTNISQTPFSMTIYKLGDTYYGARSNEFGYANYEIIPSPQFVLNPLTATL
                     NTFSIELGLNEQQKQQIVPFLQDEAKQLGALKKNTSLKPLEKIEQLKQIGSAIDAKIT
                     PLLNPEQQKKFQSLRDEARRNMIEKMGSQAIQKAEAKIKTEM"
     gene            1161..1532
                     /locus_tag="VFC15_03720"
     CDS             1161..1532
                     /locus_tag="VFC15_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09301.1"
                     /translation="MKNKVLALAAAAEAGTGLLLLAWPSLVVRLLFGADISGIGVVMS
                     RIAGIALIGLGVACWPSNSASQPLNGMLTYSTLAMLYLTYISVRGEFAGVLLWPAVVA
                     HAILSAFLVRARFKSEKTPAA"
     gene            1576..2574
                     /locus_tag="VFC15_03725"
     CDS             1576..2574
                     /locus_tag="VFC15_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZM09302.1"
                     /translation="MNEVSVVVETQPATESLRRRQPFLPRLEHWVREPLLHFLLLGVV
                     LFAAYAYMQRGRGGVEPSKQIALSLDDLRTMDMYFESQWHRQPTPAEFQAMVEDKVRE
                     EVLYREALAMGLDKDDTIVKRRMAQKMQFLAEDVAAAHEPSTAELKAWFEKNSNKFAL
                     PSRYSFRHLYFSPDKRGENAQDDAAKALAKISSQPEDSKLAASLADQFMFQDYYGDRA
                     PDALAKEFGPQFVVALEKLKPGSWQGPVESGYGWHLVYVGTVIPGRIPAFEEMEPDVK
                     TAWLAEQKKQAWQKAYTELRAKYTVLLPAPPEKGAVQTPGPPPKKQVPAPSGEGPL"
BASE COUNT          581 a          813 c          782 g          480 t
ORIGIN      
        1 gacccgacct ccggcaatct cgaccgcatc caggtcatca aaggctggac caagaacggc
       61 cagagcttcg agaagatttt cgacgtagcc tggtccggcg accgcaagcc caacaagtgg
      121 tcaggcaggg ttccggcgat cgagagcacg gtggatctgg agaaggccac ctacaccaac
      181 accgtgggtt cgacggaact gaagaccgtc tggaccgatc ccgaattcga tgccagcctg
      241 catgcgttct actacgcccg cgtgctcgaa atccccaccc cgcgctggac cctcatccag
      301 tccgtcaagg ccggcttgac cccgccggac gtggttccgc tgaccggaca ggaacgcgcc
      361 tggtcttcgc ctatctggta caccccgagc gccgatgcgc gcaagaacgc gcccgccggc
      421 atgacggtga ccgacctcaa ggcaaagagt gcaacccagt tgggcgatgc gcaactgaag
      481 gcgctgatcg tgggcaaggc cttctgggtg cgcaacaacg cgaccggtga gcagttcagc
      541 gtcgcctaca cggccgaggg taatagcaac atctggcata tcggccatag cgccaccact
      601 cccagctatg ttggcgaccc ggtgcgccat ggctatgaag gtgtaaccac tccctacaag
      661 atcgaagggg gcaaagtggt gacgaacatc tcccagactc cgttctcgat gacgatctac
      721 aagctgggtg acacctatta cggcgcgcgc agcaacgaat tcggctacgc caactacgag
      781 atcattccga gcccgcagtt cgtgctcaat ccgctgaccg ctacgctcaa caccttttcg
      841 atcgaactgg ggctgaacga acagcagaaa cagcagattg tccccttcct gcaggacgag
      901 gcaaagcaac tgggggcgct aaagaagaac acctcgctca agcctctgga gaagatagag
      961 cagttgaagc aaatcggcag cgccatcgat gccaagatca cgccgctcct caatcctgag
     1021 cagcaaaaga agttccagtc cctgcgtgac gaggcgcgac ggaacatgat cgagaagatg
     1081 ggcagccagg cgattcagaa ggcggaagcc aagatcaaga ctgagatgta gggccggaaa
     1141 gcaagaaaga atcgctgggc atgaagaaca aggtccttgc attggcggcg gccgcagaag
     1201 caggtacagg cctgcttctg ctagcctggc cgtcgctcgt ggttcgcctg ctcttcggcg
     1261 cagacatcag tggcatcggt gtcgtaatga gccgcatcgc cggtatcgcc ctgatcggcc
     1321 tgggtgtggc ctgctggccc tccaactctg cctcccagcc gctgaacgga atgctgacgt
     1381 atagcacgct cgccatgttg taccttacat acatcagcgt ccgtggagaa ttcgctgggg
     1441 tgttgttgtg gccggcagtt gtggcgcatg cgatccttag cgcttttctc gtccgcgcgc
     1501 gattcaaaag tgaaaagacg ccggcggcgt aagaacctga accgatgatc tgccgtcctt
     1561 ggttggagac agttggtgaa tgaagtgtcc gtagttgttg agacgcagcc tgcgaccgaa
     1621 tcgttgcggc ggcgtcagcc ttttcttccc cggctagaac attgggtgcg cgagccgctg
     1681 ctgcatttcc ttctgctggg tgtcgtgctg tttgccgcgt atgcctacat gcagcgcggc
     1741 cgtggcggag tcgagccttc caagcagatc gccctcagcc tcgacgacct acgcacgatg
     1801 gatatgtact tcgaatcgca atggcatcgc cagccgacgc ccgccgagtt tcaagccatg
     1861 gtggaagaca aggtccgcga ggaggttctt tatcgcgagg cgctcgccat gggcctcgat
     1921 aaagacgaca ccatcgtgaa gcgccgcatg gcgcagaaga tgcagttcct agccgaagat
     1981 gtggccgccg ctcacgagcc ttccaccgcg gaactcaagg cttggttcga gaagaacagc
     2041 aacaagttcg ccctgcccag ccgctacagc ttccgccatc tttacttttc gccggacaag
     2101 cgtggagaga acgctcagga cgacgctgct aaggccttgg ctaagatctc cagccagccc
     2161 gaagactcca agctcgctgc ctcgctcgcc gaccagttca tgttccagga ctactacggc
     2221 gaccgcgcgc ccgacgctct ggcgaaggaa ttcggtcccc aattcgtggt ggccctcgag
     2281 aagctcaaac ccggctcgtg gcaggggccc gtcgaatcgg gctatggctg gcacctagtg
     2341 tacgtaggca ccgtcatccc cgggcgcatc ccggcgttcg aggagatgga accggatgtg
     2401 aaaaccgctt ggctcgccga gcagaagaaa caagcatggc agaaggctta tacggagttg
     2461 cgtgccaaat acacggtgct gctgcccgct cctcccgaaa agggggccgt gcagactccg
     2521 ggtccgccgc cgaagaaaca agtacctgca ccctccggcg agggaccgct gtgacctgct
     2581 ttcgtgctca agctccccga cgatgtgaag aacttgcgag aacccagcgt acaggaactc
     2641 gcggactcct ttttag
//
LOCUS       DASRKK010000168         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_695259, whole genome shotgun sequence.
ACCESSION   DASRKK010000168 DASRKK010000000
VERSION     DASRKK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695259"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..560)
                     /locus_tag="VFC15_03730"
     CDS             complement(318..560)
                     /locus_tag="VFC15_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09303.1"
                     /translation="MRKASLRDLRYEFKKIERLLRQGEEIQITKRRRVIARLVPENTQ
                     SPPELPDFRARVRAIYGNKVLAVSGADLVSDDRDRY"
     gene            complement(900..2195)
                     /locus_tag="VFC15_03735"
     CDS             complement(900..2195)
                     /locus_tag="VFC15_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM09304.1"
                     /translation="MQFELFVAARYLRAKRRQAVIGVITIISIVGVAAGVASLIIALA
                     INNGFRQDLQERLLGSTSHINLLRVESDGIRNWQDLLSRLQKQPHVLAGAPAIYEQVL
                     ISRGARAQGAILKGILPQDERRVSDILDTIKSGSADSLEVRNEDQGATHPPDPDEPNP
                     LSARLTTSLPPIILGKDMADQLGATVGSLVTVTSPQGELTPFGIVPKYKRFKVTGIFS
                     SSFYEYDSSWAFIPLADAQKLFDLGDVVSVLEFKIDDIYKAPEIAQTLEAAAGKGFMS
                     TNWMEQNRAIFHALRLERVVTFITIGLIVFVAALNILISLIMMVMEKTRDIAVLVSMG
                     AKKRQIRRIFIYQGILIGVIGTVLGLIAGYLLSWAAGHYHLVSLSAEVYSIDYVPFAP
                     RALDGVLVAVVAIGVSFIATLYPSWSAASVLPAEALRYE"
     gene            complement(2552..>2911)
                     /locus_tag="VFC15_03740"
     CDS             complement(2552..>2911)
                     /locus_tag="VFC15_03740"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM09305.1"
                     /translation="AAIFFLLGFSIYRAAFQRFDRTQVSGLAELEPDRHRQQLITTGI
                     RSRVRHPIYLGHLCEIAGWCIGTGLVALYVLAVFAVVTGAVMIRMEDRELEARFGETF
                     RAYRQRVPGVLPRVGKS"
BASE COUNT          650 a          832 c          832 g          597 t
ORIGIN      
        1 ctctggcccg actcaactag ccccaccgcc tcctgcttga actccagcgt gtaccgcgcc
       61 cgcgtagact tagtcatttt cgtccccctt gttgattttc aatcattcag ctaagggata
      121 cgttttacgc gggcaaggtc attgtgccgc cgacacttcc ttccgaaaca cgcgcaggcc
      181 taaagcattc acaacttcca attcccctaa aggtgtgatc aagcggtctc ctttggaggc
      241 ttgcatcacc cggacggcag tgtcggaatt aacgtccgag ctatacagcg agacgagaaa
      301 gctggtgtcg aggtagatca atatctgtcc cgatcgtcac tgaccaggtc agcgccgctg
      361 acagccaaaa ccttgttgcc atagatagcg cgcacgcgtg ctcggaagtc gggaagctca
      421 ggaggagact gagtgttttc aggaaccagt cgagcaatca cacgccggcg tttggtgatt
      481 tggatctctt cgccctggcg caagagccgc tcgatcttct taaattcgta gcgaaggtca
      541 cggagtgagg ctttcctcat gtgatacatt ttagcatcac attgcaaggc aagagcagtt
      601 tcatattgat gaggggctgg cgctgcgtgg agcgtgcttt ctccgctacg aaaaatgctg
      661 tgcgaaaccc acggagtgta cttcttggcg ggagcccccg catttatgcg gggaaggagc
      721 gcttcagcgc tcagggaaaa gttcgtctct tgatcacgcc ctttagcgcc gggcctcgag
      781 agatcccagg gctaaagccc atttcaaaat caactgcgtt tctgctggac taaagtccat
      841 ctcccctgcg gaggcagggg gctccccagt ccgttggtct ccaggtgctc tgtgcgctgt
      901 cattcatatc gcaacgcttc ggcgggaaga acgctggcgg ccgaccatga gggatacagc
      961 gtcgcaatga aagagacacc gatggcgaca accgccacca gaactccatc gagggcgcgt
     1021 ggggcgaacg gcacgtaatc gatggaataa acttcggcgg aaagcgatac caggtggtag
     1081 tgcccggctg cccaggatag cagatatccc gcgatcagcc ccagcactgt cccgatcacg
     1141 ccgatcagga ttccctgata gataaagatg cgccggatct gtcgcttctt ggcgcccatc
     1201 gaaaccagca ccgcgatgtc gcgcgtcttc tccatcacca tcatgatcag cgagatcaag
     1261 atgttcagtg cagccacgaa cacaatcagt ccgatggtaa taaacgtgac cacccgctcc
     1321 agtcgcaggg cgtggaagat ggcgcggttc tgctccatcc agttggtgga catgaaaccc
     1381 ttgccggcgg cggcctccag cgtctgcgcg atttccggcg ccttgtagat atcgtcgatc
     1441 ttgaattcca gcacggagac aacgtcgccc aggtcgaaca gcttctgcgc gtcagcgagc
     1501 gggatgaagg cccaggagct gtcatactca tagaaactcg acgagaaaat ccccgttacc
     1561 ttgaagcgct tgtacttggg gacgatacca aatggcgtga gctcgccttg ggggctagtg
     1621 acggttacta gcgagccgac ggtcgcgccc agttggtcgg ccatgtcttt gcccagaata
     1681 atcggcggca gggaagtggt gaggcgtgcg gagagagggt tgggttcgtc ggggtcagga
     1741 gggtgcgtcg cgccctgatc ttcatttcgc acttcgagac tgtcggctga gccgctcttg
     1801 atcgtatcga gaatatcgct gaccctgcgc tcgtcttgcg ggaggatgcc cttgagaatc
     1861 gctccctggg cgcgcgcccc gcgcgaaatc aatacctgct cgtaaatcgc gggcgcaccg
     1921 gcgagcacgt gtggctgctt ctgcaatcgc gaaagcaggt cttgccagtt gcggatgcca
     1981 tcgctttcga cgcgcagcaa gttgatgtgc gaagtcgagc ccagcagccg ctcctgcaag
     2041 tcctggcgaa atccgttatt gatggcgagc gcgatgatga gcgaggcgac gccggccgcc
     2101 actccgacaa tggagatgat ggtgatgacg ccgatcacgg cctgacgccg tttggcccgc
     2161 aagtatcgcg ccgcgacaaa gagttcaaat tgcattcttg aagagctcag cgaaggttca
     2221 ttgtagcgaa aggaaggcaa ccacggattt acgcggatga acacggatct cttggatcat
     2281 ttccagagtg gcagatttac tcgctatcat cctgagtgtt acatgggccg acggcccatc
     2341 tacagggatg aaaataacca ccgagagcac ggagatcacg gagaaaactc cttaaaaaca
     2401 aactccgtgt cctccgtgtc tccgtggtga atgattttcg acggagtcga aggaccccta
     2461 aagcacggac cacgctgcat cctgaccgca aaccgtccat gcaggaccac caatcctaga
     2521 gccactctta caaatccggg taaatccgcg atcaactttt tccaactctt ggcaggacgc
     2581 cgggtactcg ctgccggtac gcgcgaaatg tttccccgaa gcgcgcttcc agttcgcgat
     2641 cttccatgcg gatcattacc gctccggtga ccacggcgaa gaccgccagt acgtatagcg
     2701 cgaccaatcc tgttccgatg caccagccgg cgatctcaca gagatgaccc agatagatag
     2761 gatggcgcac ccgcgagcga atgccggttg tgatcagttg ctggcgatgg cggtccggct
     2821 caagttcggc caagccggaa acctgggtgc ggtcgaatct ctggaacgcc gcgcggtaaa
     2881 tcgagaatcc cagaagaaag aagatggccg c
//
LOCUS       DASRKK010000169         7041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_793103, whole genome shotgun sequence.
ACCESSION   DASRKK010000169 DASRKK010000000
VERSION     DASRKK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793103"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1294)
                     /locus_tag="VFC15_03745"
     CDS             complement(182..1294)
                     /locus_tag="VFC15_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM09306.1"
                     /translation="MASKFHTSCRATLPVALFLLLASSLSAQQGPGIDPSAGPFPDAP
                     QPAQVTNPLGANDSQQRSTGGLSGTVFDTNRDVLQGARVTLADPSGSVTRTVESGSDG
                     QFAFTGLPAGVYMLTVAAPGMGTFTSIQSPLQAGEFRIVQPVTLSVSRRTINVTVTSE
                     NKEELAREQVQIAVHQRFLGVIPNFYSTYDWNAPPMVAKYKFQLSIRSIIDPVSFLAV
                     AGLAGAEQYKNVFPGYGGGVEGYGKRYGAAFANHVSSNLLGRAVYPSIFHQDPRYFYK
                     GKGTIRSRVLYALSSTLIARGDDGQSQPNYSRVLGSFSAAAISNLYYPASDRGGSLVL
                     VNGFAGLGADAVSNLIREFFLKNITSHVPKGANGQP"
     gene            1790..2812
                     /locus_tag="VFC15_03750"
     CDS             1790..2812
                     /locus_tag="VFC15_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZM09307.1"
                     /translation="MHQRTGPESDSTESVSIRSPQIASYRDAGEVRAMRFAMRISLAF
                     GVVMLVGKTAAYFMTGSAAILSDAAESVIHVVAVAFAAFSLRLSTKPAAPKFLYGYER
                     ITFFSAGFEGAMIVLAAIFILYTAIEKWLTGLRLENLGSGILMVLVAGILNAGLGWYL
                     IRAGRRNHSLILEANGKHVLTDSWTSFGVVAGLGLVMLTKWKPFDPLLAIAVAINILW
                     SGGHLVWRSAVGLLDHSDPEVGREIRDKLDSICSELGVQYHGVRFRTTGYRQLIEVHL
                     LFPHSMSVGEAHRQATLLEERLPSELSVPAEVFTHLESLEDHTAVHAKEHYTGKPESA
                     GAPSHQ"
     gene            complement(3816..4322)
                     /locus_tag="VFC15_03755"
     CDS             complement(3816..4322)
                     /locus_tag="VFC15_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZM09308.1"
                     /translation="MLWLTRLFSRSLDTAARHFGKTEPEHLVTARRGEMEAYFHLRDQ
                     GYRIVAKNFRTPHNRGEIDLIGWDHGVLCFVEVKTQARPGLTPPEMAVDTAKKMHILS
                     VAKRYLRRLPGDRTPACRFDIVSVVLRDKNGKLAIKLYKGAYSWKTSRHAEREPYAAF
                     DRKRWWRR"
     gene            4611..5183
                     /locus_tag="VFC15_03760"
     CDS             4611..5183
                     /locus_tag="VFC15_03760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZM09309.1"
                     /translation="MKSQFWTAPNQLTLLRLIFIPFVIMNVLDGEWHWALGLLVAAGL
                     SDALDGWLARALNQRTMLGQYLDPIADKLLLSSLFLVLAFAGKIPWRYTVAVLGRDVL
                     IVATCMVLYATVGFRDFRPSIFGKINTACEIGAVFFVVLAQVLDGPAVHSLEKLFLYA
                     TFVFTTISGVHYVLITGYRLRHADPKLPVL"
     gene            5183..6157
                     /locus_tag="VFC15_03765"
     CDS             5183..6157
                     /locus_tag="VFC15_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM09310.1"
                     /translation="MAVSANPGSEHRLKVVVAFGLVYVFWGSTYLAIGMTSAAGIPPF
                     VMCAMRFLIAGPLMLVACAVFGRSVRVSRQEALRLAIIGGLLLVGGNGGLAWAEQYVP
                     TGFAALIVAVTPIWFLVLETFIFRGDRMSRRGLIGLAMGIVGIAILVWPKITHRDALG
                     VMQLIGSISLLFSSMSWAIGSVFSRKWQMKVDPFVATSWEMIFAGLGHALLMLVTGQI
                     HQASFNRRGVMAVLYLVVFGSWIGYTAYIWLLKHVPTAKVATYAYVNPIVAVLLGWVV
                     LHERFDHFMLAGTIVIVAAVVMVTTAKVHTGDAIESAGEGLPELESEV"
     gene            complement(6166..>7041)
                     /locus_tag="VFC15_03770"
     CDS             complement(6166..>7041)
                     /locus_tag="VFC15_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZM09311.1"
                     /translation="PGWHIECSQMSMKYLGDSFDIHAGGEDLIFPHHENEIAQSEALT
                     GKPFVNVWFHSRFLLVEGEKMAKSAGNFFTVRDLVLMGHKPSSIRFLLMSVPYRKQLN
                     FTFDGLTQAANSVERLRNFKRRLETSQFPAGANPAIAELAEKASTEMRAAMEDDLNTA
                     RALGAAFDLVRDANAAADNGQVHKDDAPKLLKVLDQFDEIFAVLEDDDAAKVRTTVEW
                     AKAEGNADKISPVAAELAKAASLSDVDVEKLVAEHSQARKTRDFARSDAIRNQLAENG
                     VILENTKDGVRWKRK"
BASE COUNT         1460 a         1938 c         2060 g         1583 t
ORIGIN      
        1 ggaggtgtgt taagagtgcg gtccgatacc agagacgagg ctctggctat atccagagga
       61 gatgctgcga acgggctgcg acatcacctg cacgttgagc cgaagatctg agatcaccga
      121 gcgattggcg caaacgtcga accggatcga gtagtagggc aagcatgttc tcctggccat
      181 attatggctg cccgttcgct cctttgggaa cgtgcgaggt gatgttcttc agaaagaatt
      241 cgcggatcag attcgacaca gcatccgcac ccaagcccgc gaaaccattg acaaggacta
      301 atgaacctcc gcgatcggaa gccggataat aaagattcga aatggctgcc gctgagaagc
      361 tgccgagtac gcgcgagtaa ttcggctgcg actgaccatc atcgcccctg gcgatcaagg
      421 ttgaagatag cgcgtacaga actcgcgacc ggatagtgcc cttccccttg tagaaatagc
      481 gtgggtcttg atgaaagatc gacgggtaaa cagctctgcc cagcaggttg ctagatacgt
      541 gattagcaaa cgcagcgccg tatcgcttgc catatccttc aactccacca ccatatcccg
      601 ggaagacgtt cttatattgc tccgcacccg ctagtcccgc cacggctaaa aaggaaaccg
      661 gatcgatgat ggaacgaata cttagttgaa atttgtattt cgctaccatt gggggggcgt
      721 tccagtcata cgtactgtaa aagttcggaa tgacaccgag gaagcgttga tgcaccgcga
      781 tctggacctg ctccctggcc agttcttcct tgttttcaga ggtcacggtc acattgattg
      841 ttcgccggga taccgagagg gtcacaggtt ggacaatacg gaactcgcct gcctgcaagg
      901 gactctggat ggaggtgaaa gtgcccattc cgggcgcagc cacggtgagc atgtaaacac
      961 ccgccggtaa gcctgtgaac gcgaactgcc cgtcgctgcc agactctacg gtccgtgtaa
     1021 cagaacctga tgggtccgcg agcgtcacgc gcgcaccctg cagcacgtct cgattcgtat
     1081 cgaacaccgt cccagatagc cccccggtgc ttctctgttg tgaatcattt gctcccaatg
     1141 gattggtaac ttgtgccggt tgaggtgcat ccgggaaagg ccccgccgag ggatcgatgc
     1201 ccggaccctg ctgagctgag agactggaag ccagtagcag aaacagtgcg accggcagag
     1261 ttgctcgaca acttgtatgg aatttactcg ccattcccca ctctaatgct tggagccaat
     1321 ttagcaggta aatgtccgat tttgggttac cgagagatta cgaatttgtc atctctcgat
     1381 cgccagttat ttaacatcga ttgcgctaac gcgaacatcg gtgtcgctgg agcgcaaccg
     1441 cgattccggt tccgcggatt tctgacgaac aaagaccgag ttcgaggttt gactaaacat
     1501 acaaaacgta atcgacagcc ggaggcctcg agggcagcga ccggacaacg gtgcgggggg
     1561 ttatcacttc cggccagaag aatgagacga tcgagcagat cgtgaccgat tatcgttcga
     1621 acctggaatt gcgtccgtca gttggtcagt ggtgccaacc atgatggagt aggaactttg
     1681 tgcggacctc tcggccagcc tcatgtttgt ctgtgagtgc ggtcacattc cagttactgc
     1741 acgtcatggg aatatagacg ggtctgcaat tctacggagg acgtgaactt tgcatcaacg
     1801 gacaggccct gagtccgatt cgacggagag tgtttcgatc cggtcgccgc agatcgcctc
     1861 gtaccgcgac gcaggcgaag tgcgcgccat gcgctttgcc atgcgcattt cgctggcctt
     1921 cggagttgtg atgctggtcg gcaagacggc agcctatttt atgacgggtt cggcggccat
     1981 actgtcagac gcggcagagt cggtgattca cgtcgtcgcg gttgcgttcg cggcgttcag
     2041 cttacggctg agcacgaaac cagcagcccc aaaattcctg tacggctatg aacgcatcac
     2101 gttcttctcc gcaggatttg aaggcgcgat gatcgtgctg gctgccatct tcatcctgta
     2161 cactgccatc gaaaaatggc tgaccggcct gcgactggag aacttgggat cgggaatact
     2221 catggtgttg gtggcaggga tcctcaatgc cggattgggc tggtacctga tacgtgcggg
     2281 gcgacgcaac cactcgctga ttctggaagc aaacggcaag catgtactca ccgacagttg
     2341 gactagcttc ggcgtggtgg ccgggcttgg gctagtgatg ttgaccaagt ggaagccctt
     2401 cgatccactg ctggcgatcg cggttgcgat caacattctg tggtctggcg ggcacttggt
     2461 gtggcgctcg gcggtgggtt tacttgacca ttccgacccc gaagttggaa gagaaatccg
     2521 tgacaagctg gattccatct gcagcgagct gggggtgcag tatcacggtg tgcgcttccg
     2581 tacaaccgga tacaggcaac tcattgaggt gcacctgctg ttcccgcact ccatgtccgt
     2641 cggcgaagct catcgacagg cgacgttgct ggaagaacgc ttgccgtctg aactcagcgt
     2701 acccgcggag gtgtttacgc atctggagtc gctggaagac catactgccg tgcatgcgaa
     2761 ggaacactac acaggcaagc cggagtctgc aggcgcacca agtcaccagt aatacgtacg
     2821 atatcgcgac gacttctcgc cagtgccttg ctctgactgt ctactgtgtt acgagcttgc
     2881 cctcacgcac ttgccgacaa cgagcctgtg ggtcttttgg catcccgtcc gcatcacatt
     2941 caactctgaa tgcgaaatcg ggaactggat tgcaatggtg gttcgtagcc ggcgagacgc
     3001 aaaagctccc cccgaagtct tcacctggga agcctatccc cgacaatcgc tgggatttct
     3061 cccgagcggc gggcaataca ccagcaaaac gcccaggaag tcggtatctg ggcagggcgc
     3121 ggctggcgcg tcctgcgccc tgtcccggca atctgcggta ttgagaaagg tgacggtcga
     3181 atcaagcgcc agtgtcgcta ggcgccaagg cagcatgcga tggacaatcc tgtgaaaaac
     3241 cgcgtagcca agaccgtagt agatcgatgg tgctctttcc ataaggcgct cggcggaacg
     3301 aagcggaagt atccgacgcg agaattcctt tcgttcgcag aggccgcccg aagctatatc
     3361 gacttgacgc gacatgatca actcatacac agagacgtcg ccaacgccat aaaaggcttg
     3421 acagagtttc ttcggctgga gagaaagcga gtgccaggca gaattctctc tgaggcggac
     3481 cggctggagt gcctattctt cggcgggttc gatccgcact tcgaaggcga caagcctccc
     3541 ggcctgtaga gcgggaagac tgctcgccgc aacttcggcc gctagtgcgt aatacgtttt
     3601 ttgtgctgaa cagggacgcg atcgctcaca aggggttccg ccagaagaca aatcggcttc
     3661 tgcgtccgat tactccccat aaacgttagg tctctatcga aactacgtgc cttcactagc
     3721 tgcttcatta ttctatcggg gcatggtcgc cggggcaagt atgggtggcg gagcatccgc
     3781 ctctcgtgac gagtctgcca accaggcttc caacgttacc gccgccacca acgtttccga
     3841 tcaaaagcag catagggctc acgttccgca tgacggctcg tcttccacga gtacgctccc
     3901 ttatacagtt tgattgcgag cttgccgttt ttatctctaa gcacgacgct gacaatgtcg
     3961 aagcggcaag cgggagtccg atcgcccggc aggcgccgta ggtaacgttt cgcgaccgac
     4021 aaaatatgca tcttcttggc ggtatcgacg gccatctccg gaggtgtcaa gcctggtcgc
     4081 gcttgcgtct tcacctcgac gaagcagagt acgccatggt cccacccaat cagatcgatc
     4141 tcgccccggt tgtggggcgt gcggaagttc ttggcgacga tgcgatagcc ctggtcccgg
     4201 agatggaaat aagcttccat ctcgccacgg cgggcagtca caaggtgctc cggttccgtc
     4261 ttgccaaaat ggcgagcggc ggtgtccagc gaacgagaga agagtcgagt cagccagagc
     4321 atgagtttat tacggtgaat aaatccctaa gctgagactg tattcgctcc gcaggagtcc
     4381 acgcggtgat aaccgtcacc tgtcgaatga cgactcgtta ggcgggatgc acgcttgtcc
     4441 aatacgagtg ttgccaagga ggcagacaaa cattcacaat ctaaggcagg ggttgctgca
     4501 gtcgtgcgag ctgacaatat cttgtctgca gcacaatggc ctgccaaccg ggggaacgca
     4561 tcattcgtgc ctaatcgaat cgtggaaagc tatctgtcgg gagtgacagg gtgaaaagcc
     4621 agttctggac agcgcccaat cagctcacct tgttacggct gattttcatt ccctttgtca
     4681 ttatgaacgt cctcgatggc gagtggcatt gggcgcttgg tctgctggtg gccgcgggat
     4741 tgagcgatgc gctggatggc tggctggccc gcgcactcaa tcaacgcacc atgctgggac
     4801 agtacctcga tcccatcgcg gacaaactgt tgttgagctc gctcttcctg gtgctggcat
     4861 tcgcagggaa gattccatgg agatacacgg ttgcggtact tggacgcgac gtgctgatcg
     4921 tggcgacttg tatggtgctc tatgcgaccg taggcttccg cgactttcgc cccagcatct
     4981 tcggtaagat caacaccgct tgcgagattg gcgctgtttt cttcgtcgtc ctggcgcagg
     5041 tgctggatgg gccggcagtg cattcgcttg aaaagttgtt cctctatgcc acgttcgttt
     5101 tcaccaccat ctcgggcgtt cactacgtgc tgattacggg ctaccgtttg cggcacgccg
     5161 acccgaaact gccggtcttg tgatggctgt ttccgccaac cctggcagcg aacatcgcct
     5221 gaaggtcgtg gtggccttcg gactggtgta tgtattttgg ggatcgactt acctcgccat
     5281 cggcatgacc tcggccgcgg ggattccacc cttcgtcatg tgcgccatgc gcttcctcat
     5341 tgcaggcccg ctgatgctgg tggcctgcgc cgtgtttggc cgtagcgttc gcgtctcccg
     5401 gcaggaggca ctgcggctcg cgataattgg cggcctgctg ctcgtcgggg gaaatggcgg
     5461 gctagcatgg gccgaacagt acgtgcccac gggatttgcg gcgctaatcg ttgctgtgac
     5521 gccaatctgg tttctggtac tggagacttt catctttcgc ggcgaccgca tgtcacgacg
     5581 cggcctcatt ggcttggcga tgggaattgt cggtatcgcg atcctggtgt ggccgaagat
     5641 cacgcaccgc gacgcgctag gagtgatgca gttgatcggc tcgatcagcc tgctgttctc
     5701 gtcgatgagc tgggcgatcg gttcggtgtt ctcgcgcaag tggcagatga aggttgaccc
     5761 gttcgtagct accagttggg agatgatctt tgccggtctt ggccatgccc tgctgatgtt
     5821 agtcacagga caaatccacc aagcctcgtt caaccggcga ggcgttatgg ccgtgctgta
     5881 cctcgtggtt tttggatcgt ggattggcta taccgcttac atctggctgc tgaaacatgt
     5941 accaacggca aaggtcgcga cgtatgcgta cgtgaatccg atcgtggctg tcttgctggg
     6001 atgggtcgtg ctgcatgagc gcttcgatca tttcatgctg gcaggaacca tcgtgatcgt
     6061 cgcggccgtg gtaatggtga cgacggcgaa agttcatact ggcgacgcga tcgaatcggc
     6121 tggagaaggt ttacctgagc tggaatcaga agtctgaagt gcgcgtcatt tgcgcttcca
     6181 tcgaacgccg tctttggtgt tctccaggat gacgccattc tccgcaagtt gattgcggat
     6241 ggcgtcggag cgcgcgaagt ccctggtttt gcgggcctgc gaatgttccg cgaccagctt
     6301 ctccacatcg acgtcagaca gggaagcggc tttcgcgagt tccgccgcca caggactgat
     6361 cttgtctgcg ttgccttcgg ctttcgccca ctcgaccgtt gtccgcacct tggcagcgtc
     6421 gtcgtcttcc aacaccgcaa aaatctcatc gaactgatcg aggaccttca gaagtttggg
     6481 cgcgtcgtcc ttatgcacct gaccattgtc ggcggcggcg ttggcatcgc gtaccaggtc
     6541 gaaggcagcg cctaaagcgc gagcagtatt caaatcgtct tccattgccg cgcgcatttc
     6601 cgtggacgcc ttctcagcca gttcggcaat cgccgggttc gcgcctgccg gaaactgcga
     6661 ggtttccagg cgccgcttga aattgcgcag acgctcgacg gagttcgcag cttgggtcaa
     6721 gccatcgaaa gtgaagttga gctgcttgcg atagggaact gacatcagca gaaatcgaat
     6781 cgacgacggc ttgtgtccca tcaacaccag gtcacgcacg gtgaaaaaat tgcccgcgct
     6841 cttggccatc ttctcgcctt ccaccagtaa aaatcgcgag tggaaccata cgttgacgaa
     6901 aggcttgccg gtcagcgcct ccgactgtgc gatctcattt tcatggtgcg ggaaaatcaa
     6961 gtcttctccg ccggcgtgaa tgtcgaagct gtcgccaagg tatttcatcg acatctgcga
     7021 gcactcgatg tgccagcccg g
//
LOCUS       DASRKK010000170         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_206372, whole genome shotgun sequence.
ACCESSION   DASRKK010000170 DASRKK010000000
VERSION     DASRKK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206372"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1532)
                     /locus_tag="VFC15_03775"
     CDS             complement(192..1532)
                     /locus_tag="VFC15_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM09312.1"
                     /translation="MSKRTILYIVLVSTCAVLLNGVLFLRAPIVSPVELRAVFWMAVI
                     GLFVHTLTFRLSHSSTGSIAFIPFLAAGLIAPSWIAALAAVCSVLFTEIARRGSPVKA
                     VFNVMQTAFAISAATILFGFVGGTSLLSAPATGLLDGARTNALSIIVLIAVFFLINST
                     LVAGVIAVSESGNFLRVWRKHSIASLPYDLLCAPVVYLLAWVYVTSGATGAIALCIPL
                     IGVRQLYKTNSELERVNQELLELMVKAIEARDPYTSGHSRRVAHYSQLISRAIGLGGQ
                     QVERVRIAALLHDVGKIHEIYAPLLQKPEKLTPAEWEVMQGHPIKSAELVMTVSHLAD
                     IVLPVRHHHENWDGSGYPDGLAGEQIPLASRIVLFADTIDAMTTDRPYRKALGEAQVR
                     SELTKFRAKQFDPYICDKLLASPMFGLLFAPGQRIPTPQSNMPQIAKQPALRAM"
     gene            complement(1850..2614)
                     /locus_tag="VFC15_03780"
     CDS             complement(1850..2614)
                     /locus_tag="VFC15_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09313.1"
                     /translation="MALTAPGNPLSHTASSPIAGSVLPLAEAERQLRTLPGVLDVRIV
                     PSETGAVSEVHVVTTMEVTPKATVRNVETMLKASLKMSVDHRKISVATSNEKPRAQIE
                     AERNVAAQVISAPTPVAPPAPEPLKNPRLYFEDVEIQRSMAKGVTVRVTLRKGDQSFT
                     GECEGPESARSRAELAAKSAVLAIAKVERNAAAFVVEGCKLIEAFDREFMFVGISVRM
                     GRETALLTGSCEIKDSPETASVLAVLDATNRWVSRI"
     gene            complement(2837..>3033)
                     /locus_tag="VFC15_03785"
     CDS             complement(2837..>3033)
                     /locus_tag="VFC15_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM09314.1"
                     /translation="PRETEPASNVVTIAPGMRMADIERAAIVAALRETRGNRRKAADQ
                     LGIGERTLYRKLKEYEIPEG"
BASE COUNT          697 a          930 c          767 g          639 t
ORIGIN      
        1 ggcgaagtta gcaccggcat acggctgagg cgctgagaac tctcgggcga cctaacatgc
       61 cgccgtccag ctatggcttt tctctcccga gtttccggag cgctgtactg ctccccgatt
      121 ggccgagaca cttcttctga agtattatcg accatcgcgg gttagctcaa tgtcgaggta
      181 ctgtgacgcg cttacatagc gcgcagcgct ggctgcttgg cgatttgtgg catgttgctc
      241 tgcggagtcg gtatgcgttg tccaggcgcg aacaggagcc cgaacatggg actggcgagt
      301 agcttgtcgc atatgtaggg atcaaactgt ttcgctctga acttcgtcaa ctcggaacgt
      361 acttgtgctt cgccgagggc ctttcgataa gggcgatcgg ttgtcatcgc gtcgatagta
      421 tcagcgaaga gcacgatacg agacgcaaga ggaatctgtt ctcctgccag cccatcaggg
      481 tagccgctcc catcccagtt ttcatggtga tgccgaacag gcagcacaat gtctgcgagg
      541 tgagaaacag tcattaccaa ctctgcactc ttaattggat gcccctgcat cacttcccat
      601 tccgcgggcg tgagtttctc cggcttctgc agaagcggtg cgtagatctc gtgaattttc
      661 ccaacgtcgt gcaacagcgc agcgattctc acccgttcga cttgctggcc gcccaaacca
      721 atagctcgtg agatcaattg cgagtagtga gccactcgac gcgaatgacc cgaagtgtaa
      781 ggatctcgcg cctcaatggc tttcaccatc agttcaagca gttcttgatt tacccgctcg
      841 agttctgagt tcgtcttgta gagctgacgg acgccgatca gaggaatgca gagcgctatc
      901 gcgccagtag cacccgatgt gacgtatacc catgcgagca gataaaccac gggagcgcaa
      961 agcaagtcgt acggcaagct cgctatcgaa tgcttgcgcc acacacgaag aaagttgccc
     1021 gattcgctta cggcgatcac tcccgcaacc aacgtcgaat tgattaggaa aaatactgca
     1081 ataagcacga taatcgacaa cgcgttcgtg cgagctccat cgagcaatcc tgtcgcagga
     1141 gcactaagaa gcgaagttcc cccgacgaac ccaaacagaa tggtcgcagc gctgatggcg
     1201 aacgccgtct gcataacatt gaagacggcc tttaccggag aaccacgtcg tgcaatttca
     1261 gtgaacaaca ctgagcagac cgcagcaaga gctgcaatcc aactcggagc gatcaaaccg
     1321 gcggctaaga acggaatgaa ggcgatcgac cccgtgctgc tatgggacag ccggaacgtc
     1381 aacgtgtgaa cgaacaagcc aatcacagcc atccaaaaga ccgctcgcag ctccacgggc
     1441 gatacgatcg gcgcgcgcag aaacaatacg ccgttcagta gaacggcgca tgtacttacc
     1501 aagacgatgt acagtatggt gcgctttgac ataaaaagaa ggagtccccc gacccctaga
     1561 ctaggtccaa atcgcaaaag ccgcagaaac cgcgtgaccg gtgatcgcac cgatcgcctt
     1621 taataccaat cctagagcat gcattcgcgt cacccccctt tgaactactt aagtcgcgag
     1681 accactgtgg aatcgcgcaa aggtcggctc cgctcaacta ctgtcatgcc cgctcaactc
     1741 cgcttaccag agaacggggg gactcgtgtt ttgtgactac tccattgggc tgattgtttg
     1801 agctactcgc ttccgaaaat ccggaatcgt tttcaactat cgataaaaat cagatcctgc
     1861 tcacccatct atttgtcgca tcgagaacgg ccaacacact cgccgtctcc ggactgtcct
     1921 tgatttcgca gcttcccgtc agcagcgcag tctcacgtcc catgcgaacc gaaattccca
     1981 cgaacatgaa ctcgcgatcg aacgcctcga tcagcttgca tccctctacg acgaatgcgg
     2041 cagcgttgcg ctctaccttc gcaatcgcca gcaccgcact cttcgccgcc agctccgcgc
     2101 gcgagcgcgc actctcgggg ccttcgcatt ctccagtgaa agactgatcg ccttttcgga
     2161 gagtaacacg cactgtcacg cccttggcca tacttcgctg tatttcgaca tcctcgaaat
     2221 acaaccgcgg atttttcagc ggctcgggcg ccggcggtgc aacaggagtt ggcgcagaaa
     2281 ttacctgcgc tgcaacattt cgttccgctt cgatctgcgc gcgaggcttt tcgttactcg
     2341 tggcgaccga aattttccgg tggtccacgg acatcttcaa cgacgccttc agcatcgtct
     2401 cgacattccg gaccgtcgcc ttcggagtca cttccatcgt cgtcacgacg tggacctcac
     2461 tcaccgcacc cgtctcactc ggcacgatcc gcacatccaa cacaccaggc aacgtacgca
     2521 gctgccgctc cgcctcagcc aacggtaaca cgcttccagc gatcggagac gaggccgtat
     2581 gcgacaacgg attacccggt gctgtgaggg ccacggctat tcgctgaaag acgtatggtg
     2641 aacaggcggg acgtgttcgg catccttttt acatgcccgg ctcgagggga gccgcgtgcc
     2701 ttgccaagaa cagaagacac gcgtcgccaa tattcaagcg aatcaacaca aacgcaagca
     2761 ttctgtcgtt aaagaccttg aactttcgcg gcggtcaatt actgaattgt acgcaacgcc
     2821 gccatgcgta cagcgactac ccctcaggga tctcgtactc cttcagcttg cgataaagtg
     2881 tgcgctcccc aatccccaac tgatctgcgg cctttcttcg gttaccacgt gtttcccgca
     2941 gcgccgcaac gatcgccgca cgctcgatgt ccgccatccg catccccggc gctattgtga
     3001 caacatttga cgccggctcc gtctccctgg gct
//
LOCUS       DASRKK010000171         6065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_988740, whole genome shotgun sequence.
ACCESSION   DASRKK010000171 DASRKK010000000
VERSION     DASRKK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988740"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..732
                     /locus_tag="VFC15_03790"
     CDS             70..732
                     /locus_tag="VFC15_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM09315.1"
                     /translation="MRQWIIASGADTFTYPGFRDTPADRELFSVDEFVLRTAAPSQGG
                     FGVYSLPQLIDMGERNNPQTRIAWEQAKQAAEAVGIAKADLFPTLAFDTLALNGNLLF
                     GLPSNISSQGVIKVDSTIVQPSISLAWTVFDFGGNLATYDHAKYLALASKMALNQTNQ
                     KVALQIYSRLLQAADCEGAVSRGEGCRPGEPRRRRFGKGALCEWTGIDRRSCPGESPA
                     AS"
     gene            638..1282
                     /locus_tag="VFC15_03795"
                     /pseudo
     CDS             638..1282
                     /locus_tag="VFC15_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
     gene            1271..1609
                     /locus_tag="VFC15_03800"
     CDS             1271..1609
                     /locus_tag="VFC15_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZM09316.1"
                     /translation="MLDAATESFYISIYLLIGLAWFALYSAIDVFYPGSIQRSSMAMA
                     DRPSELLYFSLVTLSTIGYGDVVPLYEVRMLAALEGVTGVLYVAITVALLVSAYKQQD
                     APRERRSRTP"
     gene            1673..3067
                     /locus_tag="VFC15_03805"
     CDS             1673..3067
                     /locus_tag="VFC15_03805"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZM09317.1"
                     /translation="MESAVASFKSERRKQGKALREKCPRTAQAECKPRSKSQDIVKWL
                     EESDRDRIAGLIPLKYQRMSVSPFTFFRGAAIVQARDLANSPVSGITVQACGDCHLAN
                     FGGFASPEQKLVFDINDFDETFPGAWEWDLKRLGASLILAARDRNFSKTVADEVVRAA
                     AASYRERMSEFAEMEVIDRWYAQVSIDELKVYFRKDPAALALLSKQQKEAQSQISEAV
                     VPKYTAVVDGRHQIKDNVPVLYHFQNQKNFERETRNNMAQYEKSLPADRLALFERYLF
                     HDAAVKVVGVGSVGTRCYLGLFSSEGDEPLFLQIKEARRSVLESPRGKSRFANQGFRV
                     VTGQRLMQAASDIFLGWYRSPHGHDYYVRQFRDMKVSVEIEDFRPGNLVGYAKACGWT
                     LVRAHAKAGDAPMIAGYLGSSDDALVQYARSYADQAERDFATFQGSHSFRTPINGFEG
                     GCGSGVRAIRCSLS"
     gene            complement(3573..3953)
                     /locus_tag="VFC15_03810"
     CDS             complement(3573..3953)
                     /locus_tag="VFC15_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09318.1"
                     /translation="MMSPLKKVSLFLAPLVFFSLFPILLSAQDNMGMGGGKQAMSATG
                     CLKQGSNPGGYYLMGQDGKMYELMGPGLAAHVNHTVTVTGQQVTLSHAQEAKKEASEK
                     AEAGGKPYVDMKVSSVKMVSESCQ"
     gene            4219..4425
                     /locus_tag="VFC15_03815"
     CDS             4219..4425
                     /locus_tag="VFC15_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09319.1"
                     /translation="MTSRPNKPNAFASDFNLVRGLGVRATWSRAVPHPTKFKGGTCRG
                     PRITARADSKLAPSMARPNLFSGD"
     gene            4544..5911
                     /locus_tag="VFC15_03820"
     CDS             4544..5911
                     /locus_tag="VFC15_03820"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HZM09320.1"
                     /translation="MTTNTLIQVAGPNIKTTLPGPNARRILEGDQRYISPSYTRSYPM
                     VAKRGRGVIVEDVDGNEFLDFSAGIAVTSTGHCHPEVVAAIQKQAAELIHMSGTDFYY
                     EGMVTLGERLSQVAPMKGPHKIYYGNSGAEAVEAALKLARYHTKRQNIIAFYGAFHGR
                     TMGALSLTASKPQQKRRFSPMVPGVTHVRYPDVYRGCVGGSQEAEAFALGCARFIEEK
                     LFKTTLAPEEVAAIFVEPIQGEGGYVVAPTVFMQELRRICDRHGILLVADEVQSGAGR
                     TGKWWAIQHTGVEPDIFCSAKGIASGMPLGIMMAKAEIMDWVPGSHASTFGGNPVSIA
                     AALATMDVLEREGIKNAEVVGKHIMERIADWPQRLDLVGDVRGRGLMIGVEIVTDKKA
                     KTQGVAERDHIVELAFERGVLLLGCGPNSIRIAPPLIVTREQADIAMDLLEECITIAG
                     KERHS"
BASE COUNT         1326 a         1722 c         1731 g         1286 t
ORIGIN      
        1 acgacgctcg gcccaatctt caagctcggg actgccccat cgaaagcgaa cgtatttgcc
       61 gaagcgcaca tgcggcaatg gatcatcgct tctggtgcgg acacgttcac gtacccaggt
      121 ttccgggaca ctccagcgga ccgcgagttg ttttcagtcg atgaattcgt attgcgtaca
      181 gccgccccct cgcagggcgg ctttggtgtg tattcgcttc ctcagcttat tgatatggga
      241 gagcgtaaca atccgcaaac ccgcatcgcg tgggaacaag caaagcaggc cgcagaagcg
      301 gttggcatcg caaaggcaga tctgttcccc acactagcct ttgacacgct tgcactaaat
      361 ggcaatcttc tcttcgggct gccatcgaat atttcttcgc agggtgtaat aaaggtggac
      421 tctacgattg tccaaccttc aatctccctg gcctggacag tctttgattt tggtgggaat
      481 ctcgcaactt acgatcatgc gaagtatcta gcactcgcct caaagatggc tttgaaccag
      541 acaaatcaga aggtcgccct gcagatttac agtaggctac tacaagcggc tgactgcgaa
      601 ggggcagttt ctcgcggcga aggctgcaga ccaggcgagc cgcgacgtcg aagattcggt
      661 aaaggtgcgc tttgcgaatg gactggcatc gaccgtcgaa gctgcccagg cgagagccca
      721 gcagcgagtt agcatggccc gactcggtaa ggctgtaggg gaggtcgaaa acgcaaaagt
      781 ggagctggcg gttgcggtcg gaatgcacgc cacccagcct atcgacattc cttcgatcga
      841 tcagtcaccg tccccccagg taattgaagc atcagcaaac gatctggtgc gacaagcatt
      901 ggcaaaacgg ccagatctga ttgcggctgc agacaaaatc aaggccgccc agtaccagga
      961 gaaagaggct cgctccaaac tctttccgaa gatcagcgtc ttcgcttccg aacagtacat
     1021 acacacgacc aacaatccgc gcattcctgg agtaaccgag cttaacggga atacttacct
     1081 tgtagttggt accttacatt gggacatctt cgatggtcgc gctcactacc atcacttaca
     1141 actggccaag tcccaggtcc gcgaagcaaa gggccaactg gatagcttgc gactgcaagc
     1201 ggaacaggaa gttttaagtg catacactag cgtcgtgaac gtgctcgaag agcaggatgc
     1261 cgctcaggcc gtgctggacg cggctaccga atccttttat atcagcattt acttgttgat
     1321 tgggttggca tggtttgccc tctactctgc cattgatgtt ttctatccgg gctcaatcca
     1381 gcgcagcagt atggcgatgg cggaccggcc cagcgaactg ttgtacttca gcctggtaac
     1441 gctctcgacc atcggttatg gcgatgtagt cccgctttat gaggtgcgca tgctggcggc
     1501 cctggagggc gtcaccggtg ttctgtacgt tgcaatcacg gtggcactgt tagtcagcgc
     1561 ttacaagcag caggacgctc ctcgcgagcg tcgatcaaga acaccctgag ggagggctaa
     1621 ttcgccggcc tcagtgactg cagcatcagt ttcaacgatc aggagaatca aaatggaatc
     1681 tgctgtggca tcatttaaga gcgaaagacg caagcaggga aaagcacttc gcgaaaagtg
     1741 cccacgcact gcgcaggccg agtgcaagcc ccgctcgaag tcgcaggaca tcgtcaaatg
     1801 gctggaagaa tcggacaggg atcggattgc cggtctgatc ccccttaaat atcaaaggat
     1861 gtcggtgtcg cccttcacgt tcttccgggg cgcagctatc gtccaggcgc gagacctcgc
     1921 caactctccg gtgtcgggga ttaccgttca ggcatgcggc gactgccatc tggcaaactt
     1981 tggcggcttc gcctcgccgg aacaaaagtt ggttttcgac atcaacgact tcgatgagac
     2041 cttcccgggc gcatgggaat gggacctgaa gcggctgggg gcaagcttaa ttttggcggc
     2101 ccgggaccgg aacttttcca aaaccgtcgc agacgaggtt gtgcgagcgg ctgccgcttc
     2161 ctaccgggag cgaatgtctg aatttgccga aatggaagtg attgataggt ggtacgcgca
     2221 agtcagcatc gacgaactga aggtgtattt ccggaaagat ccggccgcgt tagccctgtt
     2281 gtcaaagcag caaaaagagg cccagtcgca aatctctgaa gcggtagttc ccaagtacac
     2341 tgccgttgtc gacggacgcc atcaaattaa ggacaatgtc ccggtcctct accatttcca
     2401 aaaccagaaa aatttcgagc gggaaacccg caacaacatg gcgcaatatg agaaatcgtt
     2461 gccggccgat cgacttgcgc tattcgagcg ctatctattc cacgatgctg cggtgaaggt
     2521 cgttggtgtc ggcagcgtag gaacacgctg ttatcttgga ctgttttcct cagaaggaga
     2581 tgagccgcta ttccttcaaa tcaaagaagc gcgcaggtcg gttctggagt cgccacgcgg
     2641 caagagccga ttcgccaatc aaggttttcg tgttgtaacc ggccagcgtc tgatgcaggc
     2701 ggccagcgac atttttctgg gctggtatcg ctcaccgcac gggcacgatt actacgtccg
     2761 ccaattccgc gacatgaaag tctccgtcga gattgaagac ttccgtcctg ggaatctggt
     2821 tgggtatgcc aaagcgtgtg gctggaccct ggtgcgggcc cacgccaagg cgggtgacgc
     2881 tccgatgatc gccggatatc tgggatccag cgacgatgcc ctggtgcaat atgcgaggtc
     2941 ctacgctgac caggcggaac gtgattttgc gacctttcaa ggcagccatt cgttccggac
     3001 gcctatcaac ggatttgaag gagggtgcgg gtctggagtt cgtgccataa ggtgcagctt
     3061 aagttgaaat ggagccatga gagacgccgg gccgggcgga gggcgcccaa actgaggagc
     3121 cgtcgagtta gaacagaaat ttcagatgca atcgaaacat cgaaaccgca tgcggtggtt
     3181 ttgcaacgac tatatcgttt tgttgtgcgc agcagctaaa gactctagag tggcgggtac
     3241 gcgccaaagt gcggccatct tgttcgatac cgccaaccca caatccaacc cacaaaatgc
     3301 gatgtcttgt acgacttgcg tgtacttagg cagtctccac gcacttgtaa gttgttgaaa
     3361 agcctatccg ggctggattg acacggtaga ggtcaggggt tcgagtcccc tcgtgcctac
     3421 catttactcc aacaactcat ggattcccca tccttttctt ccagcctggg tggaatttcg
     3481 ctagtgcccg cagacactac gagcacagtc ccatcgcgga actgtgctcg ttggtcgctt
     3541 cgctcgattc ctacaacttt cctagaacgc gttcactggc agctctcgct caccatcttc
     3601 acgctgctga ccttcatgtc cacatagggt ttgccgccgg cctccgcctt ctcactcgcc
     3661 tctttcttcg cttcctgcgc gtgcgagagc gttacctgct ggccggtcac cgttaccgtg
     3721 tgattgacgt gggcggccag accagggccc atgagttcgt acatcttgcc gtcctgtccc
     3781 atcagataat atccgccggg gttgctgcct tgcttcaggc aaccggtggc ggacatcgcc
     3841 tgcttgccgc cgcccatgcc catattgtcc tgcgcactca acaggatggg aaacagactg
     3901 aagaacacga ggggagccaa aaatagcgat acttttttca acggtgacat cattcatctt
     3961 cctttcaact ccctaacgtt ccgggagagg ttggttgggc attcatgaga tgggtacttc
     4021 gtatcgggga aaaggggctt ccgtctttca gatctatact actaccggcg atagtagata
     4081 agcaaaaggt gtctccaggg ctcgaaccag ttcacctggc cgatccccct tcggtgctct
     4141 ggcaggccga tttcttgggg gtttcttggg ggttacgccg cgctccgaag gtgactataa
     4201 tgaaagtagg agtatcgttt gaccagccgc ccgaacaagc cgaatgcctt cgccagcgac
     4261 ttcaacctgg ttcggggcct gggtgtacgt gctacctggt cacgagcggt cccccacccc
     4321 accaagttca aaggcgggac ttgtcgggga ccccggatca ccgcccgtgc agattccaaa
     4381 ctggccccca gtatggcccg tcccaacctc ttctccggcg actagctagc ttcctgttcc
     4441 cgtcggccac ccgcgccgtc tcagtccaac cagactgctt ccgcgggcag acagaagctc
     4501 cggcacacaa ctggtgccgc aagcctttcg caggagaaac atcatgacca ccaatactct
     4561 gattcaagtt gcgggtccaa acatcaagac cactctgccc ggccccaatg cccggcgcat
     4621 tctcgaaggc gatcagcgct acatttcgcc gtcctacact cgttcctatc ccatggtcgc
     4681 caagcgcgga cggggcgtca tcgttgagga tgttgacggc aacgagtttc ttgatttctc
     4741 ggccggcatc gccgtaacct ccaccggaca ttgccacccg gaagtcgtcg ccgccattca
     4801 gaagcaggct gccgaactga ttcacatgtc gggcaccgac ttctactacg agggcatggt
     4861 gaccctcggc gagcggctgt cgcaggtcgc tcccatgaaa gggccgcaca agatttacta
     4921 cggcaactca ggcgcggagg cagtcgaagc tgcgctgaag ctggcgcgct accacaccaa
     4981 gcggcagaac atcatcgcgt tctacggagc attccatggg cgcaccatgg gcgctctgtc
     5041 gctgacggcg tcgaagccgc aacagaagcg ccgtttctcg ccgatggttc ccggtgtgac
     5101 ccacgtgcgc tatcccgacg tgtatcgcgg atgcgtgggt ggaagccagg aagcggaggc
     5161 gttcgcgcta ggttgcgccc gcttcatcga agaaaaattg ttcaagacga cactggcccc
     5221 cgaggaggtg gccgcaattt ttgtcgagcc gattcagggt gagggaggct acgttgtcgc
     5281 gccgacggta ttcatgcagg aactccgccg gatttgcgac cgccacggca tcctgctggt
     5341 ggcggatgaa gtgcaatcgg gcgcaggccg caccggaaag tggtgggcca tccagcacac
     5401 tggggttgag cccgacatct tctgcagcgc caagggaatc gccagcggga tgcccctcgg
     5461 catcatgatg gcaaaggcgg aaatcatgga ctgggtgccg ggttcgcacg cttcgacttt
     5521 cggagggaac ccagtctcga tcgcagctgc gcttgccact atggatgtgc tcgaacgtga
     5581 aggcatcaaa aatgccgagg tcgtggggaa acacatcatg gagcgcatcg ccgattggcc
     5641 gcagcggctc gatctggttg gcgacgtccg cggacgcggc ttgatgatcg gagtcgagat
     5701 tgtcaccgat aagaaggcca aaacgcaagg cgtcgcggaa cgcgatcaca tcgtcgaact
     5761 cgccttcgag cgtggtgtgt tgttgctggg atgtgggcca aacagcattc gtatcgctcc
     5821 tccgctcatc gtgaccaggg agcaggccga catcgcgatg gatttgctgg aagagtgcat
     5881 cacaatagca ggcaaggagc ggcatagcta agcgattggg tagtgcgggc ctgaaggccc
     5941 gcactacccg tgcaagcgca tacacccgcg gcttgcgatt ctcttcgcgg aagaactaca
     6001 ctgccttgcg cgccgctcag cgcggcgtaa gataaaaggt tgtttgaccg aggtctgccg
     6061 tgcct
//
LOCUS       DASRKK010000172         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1379688, whole genome shotgun sequence.
ACCESSION   DASRKK010000172 DASRKK010000000
VERSION     DASRKK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379688"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VFC15_03825"
     CDS             complement(<1..1305)
                     /locus_tag="VFC15_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZM09321.1"
                     /translation="MADTKTKPVSGKLAENQETSSHVSVHPLYTPADLEGWDYDHQLG
                     FPGQFPYTRGVQASMYRGRLWTMRQYAGMGDADESNRRYKYLLSQGTTGLSIAFDLPT
                     QIGLDSDNPLAMGEVGKVGVAIDSIDDMMRLFDGIDLEKISTSMTINSTASILLALYI
                     GVARRTGRNVPKLSGTIQNDVLKEYIARGTYIYPPHQAMRIITDIFSYANQHVPEWNT
                     ISISGYHMREAGCTATQEVAFTLSNGMTYVQAAIDAGLDVDKFAPRLSFFFNAHSNFL
                     EEVAKFRAARRMWARIMRDHFHAKNPKSQMLRFHAQTAGSTLTAQQPENNIVRTALQG
                     LAAVLGGTQSLHTNSYDEALALPTEQSARIALRTQQIIAFESGAPQTVDPLAGSYYIE
                     SLTDQIEAGARTYLDKIEAMGGTLKAIERGYIQAEIQNAAYEY"
     gene            complement(1415..1783)
                     /locus_tag="VFC15_03830"
     CDS             complement(1415..1783)
                     /locus_tag="VFC15_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09322.1"
                     /translation="MMAQSENKPATSQHFGRQSRAALRVAGRKSGENRWVRALYRGGS
                     VTMASVGHVVHALWLEVTGFLFLVLAVIGAGATVREYHRYAAGTTTRGKVILAALFTL
                     LFLYFGANSFWRTRRNGNRG"
     gene            1978..2139
                     /locus_tag="VFC15_03835"
     CDS             1978..2139
                     /locus_tag="VFC15_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09323.1"
                     /translation="MFLVLEATIGFNQRDGARETPKQAESTSSGELDITSQDLKFLRS
                     LRITVNDKG"
     gene            2292..3548
                     /gene="glgC"
                     /locus_tag="VFC15_03840"
                     /pseudo
     CDS             2292..3548
                     /gene="glgC"
                     /locus_tag="VFC15_03840"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
     gene            complement(3759..>4224)
                     /locus_tag="VFC15_03845"
     CDS             complement(3759..>4224)
                     /locus_tag="VFC15_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09324.1"
                     /translation="NRFLREEYIGEFNRRFQVAPAQRGSAFVTCPRSKDLNLIFALQF
                     ERTVNRDNTVSFQNLQLQIEPVQWRGTLAGCNVMVHQHLDGSISLTHGPHRLGRYSAQ
                     GVALNVNKNAAANAVEKTRRGKVPKQTFPPRLEIPPRARDYHVSTATTATNI"
BASE COUNT          827 a         1224 c         1301 g          872 t
ORIGIN      
        1 atattcgtag gcggcgttct ggatttccgc ctggatgtag ccgcgctcga tggccttgag
       61 cgtgccgccc atggcttcga tcttgtcgag ataagtgcgc gccccggctt cgatctggtc
      121 agtaagcgac tcgatatagt acgagccagc cagtggatcg acagtctgcg gcgctcccga
      181 ttcgaaagcg atgatctgct gggtgcgtag cgcgatgcgc gccgactgct cggtgggcag
      241 cgccagcgct tcgtcatagg agttggtgtg cagcgactgg gtcccgccca gcaccgccgc
      301 cagcccctgc aatgcggtgc ggacgatgtt gttttccggc tgctgcgccg tcagcgtgga
      361 gcccgcggtc tgggcgtgga agcgcagcat ctgcgacttg ggattcttgg cgtggaagtg
      421 atcgcgcatg atgcgcgccc acatgcggcg ggcggcgcgg aatttcgcaa cctcttccag
      481 aaaattgctg tgcgcgttga agaagaacga aaggcgcggc gcgaacttgt ccacgtcgag
      541 cccggcgtca atcgccgcct gcacgtaggt catgccattg gacagggtga acgcgacctc
      601 ctgcgtcgcc gtgcatcccg cctcgcgcat gtggtagccg gagatggaaa tcgtgttcca
      661 ctcgggcacg tgctggttgg cgtaggaaaa gatgtcggtg atgatgcgca tcgcctgatg
      721 cggcggataa atgtacgttc cgcgcgcgat gtactccttc agcacatcgt tctggatcgt
      781 gccggacagc ttggggacgt tgcgcccggt gcgccgcgcc acgccgatgt agagcgcgag
      841 caggatcgag gccgtggagt tgatggtcat cgaggtggaa atcttctcca ggtcgatgcc
      901 atcgaacaac ctcatcatgt cgtcgatgga gtcgatggcc acgcccactt tgccgacttc
      961 gcccatggcc agcgggttgt cggagtcaag cccaatctgc gtcggcaaat cgaacgcgat
     1021 gctcaaaccc gtggtccctt ggctcaacag atatttgtag cggcggtttg attcgtcggc
     1081 gtcgcccatt ccagcgtact gccgcatggt ccataggcgg ccacggtaca tcgaggcctg
     1141 cacgccccgg gtatagggga attggccggg aaagcccagc tggtggtcat agtcccagcc
     1201 ttctagatcg gcgggagtgt acaggggatg gacgctgacg tgagaggaag tttcctggtt
     1261 ctccgccagc ttgccggaga cgggtttggt cttggtatcg gccatgagat taggccctcg
     1321 atgaaggtta gatgtctaaa cagcgaacat ctgattattg ccttccggct tacctcacgc
     1381 agtgagccaa gtcacaaagt ggaggtacgg gcggctaacc ccgattgccg ttgcggcgcg
     1441 tgcgccagaa cgagttggct ccaaaataga ggaacagaag cgtgaataag gcggccagga
     1501 ttaccttgcc ccgagtggtg gtgccagccg cgtagcgatg gtactcgcgc actgtcgcgc
     1561 ccgcgccaat cactgccagc accaggaaca ggaagccggt aacttccagc cagagcgcat
     1621 ggaccacgtg tcccacgctg gccatggtga cgctaccacc gcgatagagg gcccgaaccc
     1681 agcggttctc gcccgacttg cgtccggcca cccgcaacgc ggcgcggctt tgccggccaa
     1741 aatgttgcga ggtcgcgggt ttgttctcgc tttgtgccat catttgggtt aaaagcgtat
     1801 tctagcagcc agaattccgc gccaactcgt tcgcagtaaa ctgatgtttc aggtggcatg
     1861 gtggacgacg aactgaaaga gctgatgaag gaattgggag aagccatcaa ccagtcggtg
     1921 gcagagtccg accagatctc cgacgtgatg gcgcgcatca aggcgggagg ctaggacgtg
     1981 ttcctggtgc tggaggcgac catcggattc aatcagaggg acggcgcgag ggaaacaccg
     2041 aagcaagccg agagcacctc gtcgggcgag ttggacatca cgtcgcagga cctgaagttt
     2101 ctgcgatcgc tccgcatcac cgtcaacgac aaaggctgaa gttcgaatcc gcttgactgc
     2161 gccgggggcg agcgagcggt gtgctaagat ttcgtcgctc gaaggcaggt ggcccggcga
     2221 atcgcgtggc tggccttcgg tagaaacaca gtctcacccc ggttggggaa cgtagcagca
     2281 aggaaaccgc caacatgaga gataccctgg gagttttact ggcgggtggc gctggggaac
     2341 gtctatatcc cctgacccgc gatcgtgcca agccggccgt cacctttggc ggcatttacc
     2401 gcatcatcga catcacgctg tccaactgta ttaactcgga cctgcgccgg gtgtacatcc
     2461 tgacgcagta caaagcgctg tcgttgaacc gccatgtccg cgaaggatgg gggaacgtgg
     2521 tgggccgcga actgcacgag ttcatcgagg tcctgccgcc catgaagcgg gtcagcgaca
     2581 actggtacgt tggtaccgcg gacgccgtgt accagaatat ttattccatc ggctccgagc
     2641 agcccaaaca cgtgctcatt ctgtcgggcg atcacatcta caaaatgaat tacgacctga
     2701 tgctgcggca gcacaaggac tcgggcgccg acgtcacact tgcgaccatc ctggtcgatc
     2761 cctcggaggt gcggcatttc ggcgtggtcg agatcgatcg caccggccgc gtcaccggtt
     2821 tccaggagaa gcccgaacaa accgacttgc gctcgcccta cgaccaacgc atggtctcgg
     2881 cctcgatggg catctacatc ttcaacgccg acgtgctgat tccggcgttg ctgaaagatg
     2941 cggaggaccc gtcttcgcgc catgatttcg gtcacgacat ccttcccaag atggtggacg
     3001 gttcgaaggt ttactcgttc aactttatcg acgagaacaa gaagcaggcc ctctactggc
     3061 gcgacgtggg aaccctggac gcctactacg aagccaacat ggacctggta tcggtggccc
     3121 cggtgttcaa cgtgtacgac aagcactggc cactacgcac ccacatgcgg cagtatccgc
     3181 cggcgaagtt cgtgttcaac gatcccggac gcaccggcga ggcccacgac tccatcgtct
     3241 ccatgggatg cgtgctttcg ggcggctcgg tgcgcaactg cgtgctctcg ccggatgtgc
     3301 gcgtcaactc ctacagcgag atcgaaaaca gtattctgtt ctcgcatgtg gatgtgggac
     3361 ggcgctgcaa gatccgcaag gccatccttg atcgcgacgt gcacgtgccc gagggaacgg
     3421 tgattggcta cgataccgag gctgatcgcc agaagtatca cgtcaccgac agcggcatca
     3481 ccgtggtcac acgcgattat tcgctgttcg agaatccggt ggccgccaac tacttcgatc
     3541 ctgaatagtc ggaatcgcgc atgaaactcc tggcccggag caatccgggc ctttggtttc
     3601 tctgcggcgt tccctatcgg tgaacgccct ggtcgatccg aactggatgc gagaggtggc
     3661 acggttcccg gcaccttttc acttatccat gttcgtagca agttaaaatg tccgcttttt
     3721 gtagcaagtg aaatgtccgg ttttggtttt taggttgttc atatattcgt agccgtcgtc
     3781 gctgtggaaa cgtggtaatc ccgcgctctt ggcgggattt ccaagcgcgg tgggaaagtc
     3841 tgctttggga cttttccacg gcgcgtcttt tccacagcgt tcgccgccgc gtttttgttt
     3901 acgttgaggg ccacaccttg ggcgctataa cgccccagcc gatgcggccc gtgggtgagg
     3961 ctgatgcttc catccaggtg ctgatgcacc atgacgttgc agccggccaa cgtgcctcgc
     4021 cactgcaccg gttcaatctg cagctgcaga ttctgaaagc tgacggtatt gtcgcggttc
     4081 actgtgcgct caaactgcag cgcgaagatc aggttcagat ccttgctgcg cggacacgtc
     4141 acaaacgcgc tcccgcgctg tgccggcgcc acctgaaagc ggcggttgaa ctcgccgatg
     4201 tattcctccc gcagaaaccg gttg
//
LOCUS       DASRKK010000173         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_500262, whole genome shotgun sequence.
ACCESSION   DASRKK010000173 DASRKK010000000
VERSION     DASRKK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500262"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFC15_03850"
     CDS             complement(<1..359)
                     /locus_tag="VFC15_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09325.1"
                     /translation="MKLSAKKTTNVNGVKAVSAATLKLRLLKSLKCTLKYEKLAWDSG
                     AKLVAGVDEVGRGSLFGPVVAAAVILDPSYRIRGLRDSKLLDRETREKLAPRIREHAL
                     AWAIAAVDVATIDQINI"
     gene            complement(730..1977)
                     /gene="ispG"
                     /locus_tag="VFC15_03855"
                     /gene_synonym="gcpE"
     CDS             complement(730..1977)
                     /gene="ispG"
                     /locus_tag="VFC15_03855"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZM09326.1"
                     /translation="MAQIQRRKTYTALVAGVRVGSDAPIVVQSMTNTDTADIDGTIRQ
                     CAALARAGSELVRVTVNNDAAAAAVPHIVDGLDRLGFPVPIIGDFHYNGHILLKKYPA
                     CAQTLAKYRINPGNVSIGKKDDDNFRTMIEVAMENNKPVRIGVNWGSLDQSLLTRLMD
                     ENSKLDDPKDARDVTMEAMVLSALRSADLAVSYGLRKDQIILSAKVSGVQDLIDVYRE
                     LGRRCEYPLHLGLTEAGMGAKGIVSSTAALAVLMQEGLGDTIRVSLTPAPNGDRTEEV
                     TVAQQILQSIGIRSFNPQVTACPGCGRTTSTFFQEMAEQIQSYLREQMPVWKTRYSGV
                     EEMKLAVMGCIVNGPGESKHSNIGISLPGTFEEPKAPVYVDGRLMLTLKGDHIVEEFI
                     KILDEYVDSHYAAKEQKLVGISN"
     gene            2313..>2872
                     /locus_tag="VFC15_03860"
     CDS             2313..>2872
                     /locus_tag="VFC15_03860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZM09327.1"
                     /translation="MRCLSRFLCCGALALLLVPFGVAQSQSSSSQDPNATSNPPAQDT
                     SNPPVPEKPDKNAPSLSTLKNIDAIGNRNVGCNKGMGNWYSLDKQVAMGRAYSQQVEH
                     GAKMVNDPVVTEYVNRIGQNLVRSSDAKVPFTIKVIDTDEINAFALPGGFFYVNSGLV
                     LAADNEAELAGVMAHEIAHVAACHVAR"
BASE COUNT          598 a          842 c          835 g          597 t
ORIGIN      
        1 taaatgttga tttgatcgat ggtggcgacg tccaccgccg cgatcgccca agccagcgcg
       61 tgctcgcgaa tccgcggcgc cagcttctcg cgcgtttcgc ggtccagcaa cttggaatcg
      121 cgcagcccgc gaatgcgata gctcgggtcg agaatcacgg cggccgcaac caccggtccg
      181 aagagcgatc cccggccgac ttcatccacg ccggccacga gtttcgcgcc cgagtcccac
      241 gccagctttt catacttcag cgtgcacttc agactcttca acaggcgcag cttcagcgtg
      301 gcggcggaga cggccttgac gccgttaacg ttcgtcgtct ttttcgcgga caatttcatg
      361 attgagtaag aaagcagatc cgatagggaa attctagagc agattttgcg aacagatgcg
      421 agaggcggca ggaaattgcg aacattgccg acgggctaag tggcttgccg ctcggatcgc
      481 agttgcgaac tcgccagcaa taataagtag gtggcagcgt ggaacaggaa aaagcaggtc
      541 tctccgctcg ggctgacaaa ataaaaagaa gaactcggac gctggcctga aggccagctc
      601 tacctcaagg ccaccttctt atttacaaac tataggccac tcggtcgcga tgacaaaagc
      661 gaaattgtcg cccgctctac cggatggctg aaacctgatt gctaagtgct aaatgctaat
      721 tgctaaatgc taattgctaa tccctaccag cttctgctct ttcgccgcgt agtgcgaatc
      781 cacgtactcg tcgaggatct tgatgaactc ctcgacaatg tgatcgccct tgagcgtgag
      841 catcaagcgg ccgtccacat aaaccggggc cttcggttcc tcgaatgtgc ccggcagcga
      901 gatgccgata ttggagtgct tggactcacc ggggccgttg acaatgcaac ccatcacggc
      961 gagcttcatc tcttccacgc ccgagtagcg cgtcttccac accggcatct gctcgcgcag
     1021 gtagctctgg atttgctcgg ccatctcctg gaagaaggtg ctggtggtac gcccgcaacc
     1081 ggggcaagcc gtaacctgcg gattgaagct gcggatgccg atggattgca gaatctgttg
     1141 cgcgaccgtg acctcttcgg tgcggtcgcc attgggcgcg ggtgtgagcg acacgcgaat
     1201 ggtatcgccg aggccttctt gcatcagcac cgccagcgca gccgtgctgc tgacaatgcc
     1261 tttggctccc atgccggcct cggtgaggcc gaggtgcagc gggtattcgc accggcgccc
     1321 cagctcgcgg tagacgtcga ttaggtcctg cacgccgctg accttggcgc ttaggatgat
     1381 ttggtccttg cgcaaaccat agctgaccgc caggtcggcg gagcgcagcg cgctcaagac
     1441 cattgcttcc atggttacgt cgcgcgcgtc cttgggatcg tccagcttgg agttctcgtc
     1501 catcaggcgc gtcagcagcg actggtccag cgaaccccag ttgacgccga tgcgcaccgg
     1561 cttattgttt tccatggcaa cttcgatcat cgtgcggaag ttgtcgtcgt ccttcttccc
     1621 aatcgagaca ttgcccggat tgatgcgata tttggccagc gtctgcgcgc atgcgggata
     1681 cttcttcagc aggatatggc cgttgtagtg gaagtcgccg atgatcggca ccggaaagcc
     1741 aaggcggtcg aggccgtcca caatgtgggg tacggcggca gcggcggcgt cgttgttaac
     1801 ggtgacgcgc accagttcgg aaccggcacg cgccagggct gcacactggc ggatggtgcc
     1861 gtcaatatcg gcggtgtcgg tgttggtcat ggattgcacc acgattggtg catcgctgcc
     1921 aacccgcact ccggccacta aagctgtgta agtttttctg cgctgaattt gagccataca
     1981 ggactgctta aaagtttaac acggcgggtc cgaaggcccg cactacccgt gcaagcggat
     2041 acaccaatcc cggaaccgcc cagccccgct gataggccca actgaaacga attttggaac
     2101 tcagccgtcc aatatatgga accctttgtg gaggcggctc atctgactat cgagagcctc
     2161 gaattaccgt tgggcttttg ccggtaaccc cacgaaaata aaaggttcac cgggcaacaa
     2221 atccgcccgc tttgggttta taacagggct taggggtatc aaaggctggg gtctatctct
     2281 ggcccgcgat ccggcccgaa aaggtgtaca tcatgcgttg cctatcgcgt ttcctctgtt
     2341 gcggcgctct tgcgctgctg ctggttccgt tcggcgttgc tcagagccaa agctcttcat
     2401 cgcaagaccc gaacgccacg tcgaatccgc ctgctcagga cacctcgaat ccccccgtgc
     2461 cggagaagcc ggacaagaac gcacccagtc tcagcaccct gaaaaacatt gacgccatcg
     2521 gcaaccgaaa tgtcggctgc aacaaaggaa tgggcaattg gtactcgctc gacaagcagg
     2581 tcgccatggg gcgtgcgtac tcgcagcagg tggagcacgg cgccaagatg gtcaacgacc
     2641 cggtcgtcac ggaatatgtg aaccgcatcg ggcaaaacct ggtgcgcagc tcggatgcga
     2701 aggtgccgtt cacgatcaag gtgattgaca ccgacgaaat caatgccttc gcgctgccgg
     2761 gcggattttt ctacgtaaac tctggactcg tcctggccgc ggacaatgaa gcggagttgg
     2821 ccggtgtcat ggcgcatgag attgcccatg ttgccgcatg tcatgtggcg cg
//
LOCUS       DASRKK010000174         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_989078, whole genome shotgun sequence.
ACCESSION   DASRKK010000174 DASRKK010000000
VERSION     DASRKK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989078"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFC15_03865"
     CDS             complement(<1..716)
                     /locus_tag="VFC15_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09328.1"
                     /translation="MEIDQANESDWIAAEKNPSTLVEQVLRRYLADRGQSALAEHFEL
                     SLTLGEAIVDSIYGEAEPTTSGQLFFVLNTESSFALGVGNAITELEGACAGMGQAFYD
                     GLRQALYRWIRVYDDMDTRERIEQMTEWAEGEDDPESYEIPKLEQDLPPSLKDERRSN
                     TARSLTSFPVPDVPGLRELVEATIELHRVSHSMERPRVDEEWLERERHYHSLDLPLPA
                     VLLYFRAGDAVTNDFFKEALL"
     gene            complement(1024..1227)
                     /locus_tag="VFC15_03870"
     CDS             complement(1024..1227)
                     /locus_tag="VFC15_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein C"
                     /protein_id="HZM09329.1"
                     /translation="MKISLVEREFVYHGTTLACPNPKFSIEEVRSFYAAQFPELTNAA
                     ITGPETVGDRLRYSFERAIGSKG"
     gene            complement(1224..1676)
                     /locus_tag="VFC15_03875"
     CDS             complement(1224..1676)
                     /locus_tag="VFC15_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein E"
                     /protein_id="HZM09330.1"
                     /translation="MFAELMPLLRKRRLILTISLVEGDTIRATVAPQKASETEDNALT
                     TPLAITGTAEELDRDLPQQLVEFTGAHLQLQTTLASAKAEMDAAAKAAREEARKKTSK
                     PSSASSPTTPAAPAATPVSREPEAPSLFAPQAQPGTTAAEDEEGGEAS"
BASE COUNT          418 a          591 c          696 g          522 t
ORIGIN      
        1 agcaaagctt ccttgaaaaa atcattcgtg acggcgtcgc ccgcccggaa gtacagcaat
       61 acagcgggca gtggcaggtc aagcgaatgg taatgacgct cgcgttcgag ccactcctcg
      121 tcgaccctgg gccgttccat cgagtgggat actctgtgta gctcgattgt ggcttcgacc
      181 aactccctca aacctgggac atcaggaact ggaaacgagg tcagcgaccg agccgtgttg
      241 gaacggcgtt cgtccttcag ggacggtggc aggtcctgct cgagctttgg gatctcgtaa
      301 gactcaggat cgtcctctcc ctcggcccac tcagtcattt gctcgatgcg ttcccgggtg
      361 tccatgtcgt catagacacg gatccagcgg tatagggcct gccgcagccc gtcatagaac
      421 gcctgtccca taccggcgca ggcaccttca agttctgtga tcgcgttacc cacgcccaag
      481 gcaaaggacg actccgtatt gagaacgaag aaaagttggc cggaggtggt tggctccgcc
      541 tcgccgtaga tgctgtcgac gattgcttcc ccgagcgtca gagaaagttc gaagtgttca
      601 gcaagggcgg attgaccacg gtctgccaga taacggcgga gcacttgctc gaccagcgtg
      661 gaagggtttt tctccgctgc gatccagtcc gattcattgg cttggtcgat ttccaccagg
      721 gtttgggcga gtggggtaag ggcggacgag ccgaagctga tcttattgcc ggcatgacct
      781 tggttctagc cgcggaattc cgatcaggct ggcgggattg gccagatacc gggtccgggg
      841 aagacgggat ctccacttcc ttgctgtgag agagcccgtg cgagggcact gcttgcttct
      901 tgcgagtctg ggcattcgag cgactcctgc ataagtttgg tcagagggtt ggcgctggcc
      961 tgccgacccg ctgattcgcg gatttcctgt aacagcgatt gtttgctggc ttccggcata
     1021 tcatcagccc ttcgatccga tggcacgctc gaagctgtaa cgcagccggt cgccaaccgt
     1081 ttcaggtccg gtaatggctg cgttggtcaa ctcgggaaac tgagcggcgt agaacgatcg
     1141 gacttcttcg atcgagaatt ttgggttcgg gcatgccagc gtagttccgt ggtagacaaa
     1201 ttctcgttcg accaggctga ttttcatgat gcttcacctc cctcctcgtc ttctgcggct
     1261 gtcgttcccg gctgcgcttg tggcgcgaac agcgagggtg cctctggttc ccgagagacg
     1321 ggcgtcgccg ctggtgccgc tggcgttgtt ggactgctcg cgctggatgg ctttgaggtt
     1381 ttcttgcgtg cctcttcgcg agccgctttg gcggctgcat ccatttcggc tttggcagac
     1441 gccagggtgg tttgtagttg caggtgagct ccagtgaatt ccaccagctg ctgagggagg
     1501 tcgcggtcaa gttcctcagc tgtgccggtg attgccaatg gggtggtgag ggcgttgtct
     1561 tcggtttcag acgccttctg cggagcgacg gtggctctga tcgtatcgcc ctcaacgagg
     1621 gagatcgtca gtattaaccg acgtttgcgc agcaatggca tgagttctgc gaacatggtt
     1681 tttcctccgg tgtggttctg ttgcgggatc ctaatgagga tttgaatcgg ggaacgatgg
     1741 cctacaaaga tgagttatga cgggaacgtc gacacgaagg actatccgcg aatactggtg
     1801 ggattcttag agctcgcaga aactagacct cagcactccc agaagccccg caggtcggag
     1861 cttgaagtcg atttcatatt caagcaacgt gacgttcggg actggaaggg cggcatagcg
     1921 caatttgtcg tccatcgact ttgcgccaat caagacgtgc acacgggttg catccaaaga
     1981 gaattagtct aggcccgttc ggcgggcttc ttagtgggct tcaaccgctg aaggtgcgat
     2041 ggaggaggga ggcgaagagg tgctcggctt gacgcaacgc ctcgcgcgta gccatacaag
     2101 gtgaggtaga gttgacgacc ctcgcttggc aggttgctga tgttgacgga gagcccgaaa
     2161 ctttgtgcca ccgccaagtc gctccccggg cttcgatcag ctatcctttc ggttggttcg
     2221 aggattg
//
LOCUS       DASRKK010000175         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_402946, whole genome shotgun sequence.
ACCESSION   DASRKK010000175 DASRKK010000000
VERSION     DASRKK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402946"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1604)
                     /locus_tag="VFC15_03880"
     CDS             complement(195..1604)
                     /locus_tag="VFC15_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09331.1"
                     /translation="MNRRLFWKIFLPFWLAQAVLLGALYLRIHYRISSEHPWWIQPER
                     REMPVLADYAANVYEKQGQAGLSQLLDNLSLQNRSKFWLVDASGHELLGRPLPERILR
                     DASAAEKSEGLHHSFEANVLAASTTTPKAHYVLIAELVPPKLSERVPGDILWTLKLGT
                     VLSAIICFLIAHYLSKPIERLRDATHELARGNLDIRAGENLGNRRDEIADLVRDFDSM
                     AGELRTLIQSERNLLSGVSHELRSPIARIRLALTLARNADDAERSEMLDRIEQDTIQL
                     DSMLEQILTVARLESGQHKPRFEQLSLNDVVDDVLHDANFEAAATNATIAFQGDDIQV
                     NGDAGLLRSAIENIVRNAIFYSGQGGKIEVRLNKENGTALISVRDNGPGVPENALPLL
                     FKPFYRVDDSRGTTTGGMGLGLAIVRNAVLAHGGSVSAKNVVPHGLEVELRLPIPSAS
                     QPTRGSGSHRLVQTTGTTT"
     gene            complement(1601..2332)
                     /locus_tag="VFC15_03885"
     CDS             complement(1601..2332)
                     /locus_tag="VFC15_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM09332.1"
                     /translation="MNPVNRLLIIDDDHGLVNLLKRFLEGEGFHVDAAYDQASGLSAA
                     LSNDHELIILDVMLPGGTGFELLKALRLQSSVPVLLLTARGEAVDRILGLEIGADDYL
                     AKPFDPRELVARIRAIFRRTRETPAAATARNDHEEVLSVGDISMSLGTRTVTCSGTPV
                     DLTSVEFNVLELLLRNAGSVVTREQIAEIALGRPLNIFDRSVDVHVSRLRKKLGTCHG
                     TDELIRPIRGTGYFLAAKTPEKSRT"
     gene            2563..3720
                     /locus_tag="VFC15_03890"
     CDS             2563..3720
                     /locus_tag="VFC15_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM09333.1"
                     /translation="MKTRAFWGLGLTLAICAALAGCSSQQAKSATASGPPPAVPVGVA
                     TVQQSNFNVYLTGLGSVQAFNTVSLKTRIDGQIMQVNFQEGQDVKVGELLIVVDPRPY
                     QVALQQSQANLQKDEAQLTNAKAQYARNEVLYEQGVIAKQDLDTLEASLGTYEGTIAS
                     DQAAIANAKLNLTYCYIKSPINGRIGLRQVDSGNYVTAAGGTPMLVITQLHPIAVVFT
                     LPEDQLQVVSQQMKQGALTVDAYSRDDQTKLGTGKLLTIDNEIDQTTGTAKLKGVFDN
                     PESTLWPNQFVNVHLLLETHKDAITAPASAMQRGPDGTFAYVVDANNTVQMRPVKVDL
                     TQGSTVVVASGLQAGEKVVTDGQEKLQPGSHVAPQAPARQNQGSGNIGSQT"
     gene            3717..>4408
                     /locus_tag="VFC15_03895"
     CDS             3717..>4408
                     /locus_tag="VFC15_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM09334.1"
                     /translation="MSISRPFILRPIATSLLMTGLLLVGIVAYRQLPISALPQVDYPT
                     IQVVTFYPGASPDVTASSITAPLERQFGELPGLSQMTSSSSFGASVVTLQFTLEENID
                     VAEQEVQAAINSAQTYLPADLPTPPIYSKVNPADAPILTLAMTSDSMPLPQVEDLADT
                     TFAQKISQLPGVGLVSISGGQKPAVRIMANPTALASYDMSLEQLRTVIAQVNVDQAKG
                     QLQDNRQAFTIGA"
BASE COUNT          922 a         1320 c         1295 g          871 t
ORIGIN      
        1 tgcttgagac gaagtgcgag ttccctcagg ggctaaagcc caactctccg cagacataac
       61 ggcacggctg aagccgtgcc ccttcaaagc gtgaaatcct catgaatagt ccatgttaat
      121 gccacattgc ccgtacaagc ggatacacca atcctggaac cactccagac tggtcgcgcg
      181 ggtggcgccg cgctctaggt tgttgtgccg gttgtctgca ccaggcggtg cgagccggag
      241 ccacgggtcg gttgtgacgc cgacgggatc ggcaggcgca gttccacctc aagcccgtga
      301 ggcactacgt ttttcgcgga gacgctgccg ccgtgcgcga gcacggcatt acgcacgatc
      361 gcaagcccaa gtcccattcc gccagttgtc gttccccgcg aatcgtctac gcgataaaag
      421 ggcttgaaca acagtggcaa cgcgttctcg ggaacccccg ggccattgtc acggacggaa
      481 atcagtgccg tgccgttctc tttgttcagc cgaacctcaa tcttcccgcc ttggccggag
      541 taaaaaatcg cattgcgcac gatattttcg atggcgctac gcaacaggcc agcgtcgcca
      601 tttacctgga tgtcgtcgcc ttgaaaggct atggtcgcgt tcgttgccgc tgcctcgaag
      661 ttcgcgtcat gcagcacgtc gtccaccacg tcgttcagcg agagctgctc gaagcgtggc
      721 ttgtgctgtc cgctctccag ccgtgcaacg gtcagaattt gttccagcat ggaatccaat
      781 tggatggtgt cctgctcgat gcgatcgagc atctctgagc gttcggcgtc atcggcattg
      841 cgcgccagcg tcaaagccag ccggatacgt gcaatgggtg accgcagttc gtgcgagacc
      901 ccgctcagca ggttccgttc actttggatc agggtccgca actcgcccgc catcgagtcg
      961 aaatcgcgca ccaggtcggc aatttcgtcc cgccggttgc cgaggttctc tccggctcgg
     1021 atgtcgaggt tcccacgtgc gagttcatgg gtcgcatcgc gcaggcgttc gatgggcttg
     1081 ctcaaataat gcgctatcaa gaaacaaatg atggccgaca agacggtgcc gagcttcaag
     1141 gtccacagga tgtctccagg aacgcgctcc gacagcttgg gcggcacaag ctcggcgatc
     1201 aggacgtagt gtgccttggg cgtggtcgtc gaagccgcca ggacattggc ttcaaaggaa
     1261 tggtgcaggc cttccgactt ctcggctgcg gaggcatcgc gaagaatgcg ctccggaagc
     1321 ggccgcccca aaagctcatg cccgctggcg tcgaccaacc agaacttcga gcggttctgc
     1381 aacgacagat tgtcgagtaa ctgtgacagt cccgcctgcc cctgcttttc gtacacattc
     1441 gctgcgtaat cggctagcac cggcatttcg cggcgttcgg gctggatcca ccagggatgc
     1501 tcggaagaga tgcggtagtg gatgcggaga tacagcgctc ccagaagcac cgcctgcgca
     1561 agccagaagg gaagaaagat tttccagaac aggcgccggt tcatgtcctg ctcttctccg
     1621 gcgttttggc cgccagaaaa taaccggtcc cgcgaatcgg ccggatcagc tcatcggttc
     1681 cgtgacaagt gcccagtttc ttgcgtagcc ggctgacgtg aacatcgacg ctgcgatcga
     1741 atatgttcaa aggccgcccc agcgctatct cagcgatttg ctctcgggtg acgacgctgc
     1801 cggcgttgcg cagcagtaat tcgagaacgt tgaactccac cgaggtgaga tcgaccggcg
     1861 tacccgaaca ggtgaccgtg cgcgttccga gcgacatgct gatgtcaccg acgctgagga
     1921 cttcctcatg atcgttgcga gcggtggccg ccgctggcgt ttcgcgcgtc ctgcgaaata
     1981 tggcgcgtat ccgggccacc agttcgcgag ggtcgaaagg tttcgccagg tagtcatcgg
     2041 cgccgatttc aaggccgagg attcggtcga ccgcttctcc tcgggctgtc aacagcagca
     2101 cggggaccga ggattggagg cgcagcgcct tgagtaattc gaatcccgtg ccgcccggca
     2161 acatcacatc gaggatgatc aactcatgat cgttggatag cgcggcgctc aatcccgatg
     2221 cttggtcgta ggcggcatcc acgtgaaagc cttcgccctc aagaaagcgc ttcagcagat
     2281 ttaccagacc gtggtcgtca tcgatgatga gcagacgatt cactggattc attctgcctt
     2341 ttcccgcgcg tgcgtgcggt gaagaattgt ttcgggatgt attagggcct tcacaaatct
     2401 taactttcct tcaccctctc ttaatggctg tcgctgcccc cgtccattct aatagtatgg
     2461 ttcatagtaa ctgcaactta tgcgccgggt tgcatttgtg cggcaagggc gagcgcggtg
     2521 gcatctctgt agagatggca tagcgctatt taggaggagt ttgtgaagac acgggcattc
     2581 tggggactgg gactgacgct ggcgatttgc gctgcgctag ccggttgcag ttcacagcag
     2641 gcgaagagcg ccacagccag cggcccgccg ccggcagttc cggttggagt cgccacggtg
     2701 cagcaaagca attttaatgt ttatctcacc ggactaggct cggttcaggc attcaacacc
     2761 gtttccctga agactcgaat tgatggacag atcatgcagg taaatttcca ggaaggccag
     2821 gacgtgaagg taggagagct tctgatcgta gtcgacccgc gtccttatca ggtggcgttg
     2881 cagcagtccc aagccaactt gcagaaggac gaagcccagt tgacgaatgc caaagcgcaa
     2941 tacgcgcgca acgaagttct ctacgaacag ggcgttatcg caaagcagga cctggacacg
     3001 ctggaggcat ccttaggaac gtacgaagga accatcgcca gcgaccaggc ggcgatcgca
     3061 aacgccaaac tgaacttgac ctactgctac atcaagtccc ccattaacgg acggattggc
     3121 ctgcgccagg tcgattccgg caactacgtt accgcagctg gcggcactcc catgctggtc
     3181 atcacccaat tgcatcccat cgcggtggtg ttcaccctac ccgaagacca actgcaagtc
     3241 gtctctcagc agatgaagca gggcgcgctg accgttgacg cttacagccg tgacgatcag
     3301 accaagctgg ggaccgggaa attgctcacc atcgataacg agattgacca gacgacgggc
     3361 acggccaagt tgaagggggt cttcgacaat ccggaaagca cgctgtggcc caaccaattc
     3421 gtcaacgttc atctgcttct ggaaacccat aaggacgcca tcacggcgcc ggccagcgcc
     3481 atgcaacgcg gtcccgacgg cacttttgcc tacgtggtgg acgccaataa caccgtacaa
     3541 atgcggccgg tgaaggtcga tctcacccag ggcagcacgg tggtggtcgc gtcgggcctg
     3601 caagcaggcg agaaagttgt gacggacggc caggagaaac tgcagccggg aagccacgtg
     3661 gctccgcaag cgcccgcccg ccaaaatcag ggcagcggaa acatcggatc gcagacatga
     3721 gtatttcgcg gccattcatt cttcggccca tcgccacgtc actgctgatg accgggctgt
     3781 tgctggtggg catcgttgcc taccggcagc tgccgatctc cgccctgccg caggtggact
     3841 atccgacgat ccaggtggtg acgttttacc ccggcgccag ccccgatgtc acggcgtctt
     3901 cgatcacggc accgctggag cgccagttcg gcgaacttcc cggtctcagc cagatgactt
     3961 ccagcagctc cttcggagcg tcggtcgtca ccttgcaatt cacccttgaa gagaacattg
     4021 atgtcgccga gcaggaggtg caggcggcca tcaattctgc gcagacgtac ctgcctgccg
     4081 acctgccgac cccgccgatt tacagcaaag tgaatccggc cgacgcgccc atcctgaccc
     4141 tggcgatgac gtcggacagc atgccgcttc cgcaggtgga agatctggcc gacacgacct
     4201 tcgcgcaaaa gatttcgcag ttgcctggtg ttggactggt gagtatcagc ggcgggcaaa
     4261 agcccgcagt tcgcatcatg gcaaacccaa cggcgctggc ctcgtacgac atgagcctgg
     4321 agcaactgcg gacggtgatc gcccaggtga acgtcgacca ggcgaagggg caactgcaag
     4381 acaaccggca ggcctttacc atcggcgc
//
LOCUS       DASRKK010000176         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_305002, whole genome shotgun sequence.
ACCESSION   DASRKK010000176 DASRKK010000000
VERSION     DASRKK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305002"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..217
                     /locus_tag="VFC15_03900"
     tRNA            143..217
                     /locus_tag="VFC15_03900"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Asn,seq:gtt)
     gene            complement(220..543)
                     /locus_tag="VFC15_03905"
     CDS             complement(220..543)
                     /locus_tag="VFC15_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09335.1"
                     /translation="MLKNLTEVRKIAILLFVGLFACYLSLSSGSIAGQGYVGEEIESG
                     LRMLAVVNAWLKGLPMPQMEWSRHEPVPVLFDLPFIKLGKIFVSHSFVLSKRARINRR
                     PAQCR"
     gene            complement(643..1326)
                     /locus_tag="VFC15_03910"
     CDS             complement(643..1326)
                     /locus_tag="VFC15_03910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09336.1"
                     /translation="MSPAEFDEFASKYEQILEGPCALSGESPDFYAEERFHWSLRRLI
                     RWLPFETALDFGCGIGGSIPYLFQTLGCKRVVAVDPSVESLRVARERYSEFDVRLMTP
                     EEFAPSSNIPFAFCNGVFHHIPPANRLQALAYIRAALSENGILAFWENNPWNPLVAYG
                     MSLNEFDRNAHTLSAPHAVRLLKTAGFRVQFVDFCFFFPHFARMFRGLEPTLRWLPLG
                     AQYLVLAQK"
     gene            complement(1323..>2142)
                     /locus_tag="VFC15_03915"
     CDS             complement(1323..>2142)
                     /locus_tag="VFC15_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM09337.1"
                     /translation="SRFKVVRADAKDVNQLTAAMSGHDVVIHLASNPDIARAATEPDI
                     DFREGTYLTNNVVEAMRRSGTATILYASGSGVYGDLGEKEADEDYGPLIPISTYGASK
                     LAGEALISSYCHMFDFTGRAFRFGNVVGPRQTHGVGFDFLRQLLKDPRKLHILGDGTQ
                     SKSYIHVRDVMEAVLLAARRSTKRFAIYNVATGDYITVKEIAELAVECVGLNQEQVEF
                     EYTGGNRGWKGDVPIVRLNTDRIRGLGWTCRAGSREALRDSMLAMLPDLKAGRL"
BASE COUNT          500 a          622 c          582 g          438 t
ORIGIN      
        1 tgtgtaggct catagaaatc tgctgtcgcg ctccaaatcg gaacgcgatg ttgaacgaaa
       61 gcttatttca gttttgacgc aatcggcacc tgccgctaca atatctttac aacttgaaac
      121 gctgtcgtca gcgcaccaat actcctcagt agctcaatgg cagagcatcc ggctgttaac
      181 cggagggttg taggttcgag tcctacctga ggagccattc tatctacact gagcggggcg
      241 acgattgatc cttgccctct tggacaagac gaagctatga gatacgaaga tcttgcctag
      301 cttgatgaag ggaagatcga acaataccgg cacgggctcg tggcgggacc attccatctg
      361 gggcataggg aggcctttga gccatgcatt cacgaccgcc agcatgcgca ggccggactc
      421 tatctcctcg ccaacatagc cttggcccgc gatggaaccc gaagacaatg acagatagca
      481 ggcgaacaag cccacgaaca gtagaatcgc gattttgcgt acttcggtta aattcttcag
      541 catgtcacgt gttgattaga ctgcgcattc gattcccagc caccatccgc actcgccagc
      601 aggaggagcg atcggcatgc gctgattgca ggacggtaca tgtcactttt gcgccagcac
      661 gagatactgc gcccccagcg gcagccatct caatgtcggc tccaatccac gaaacattcg
      721 ggcaaagtgc ggaaagaaaa agcaaaaatc gacaaactga acgcgaaacc cggcggtctt
      781 gagcagacga accgcgtggg gagctgaaag cgtatgcgcg ttgcggtcga actcgttcag
      841 cgacatgccg tacgctacaa gcggattcca ggggttgttt tcccagaaag ccagtatccc
      901 attttcggac aaagctgcac gtatatatgc gagtgcctgt agccgattgg cgggtggaat
      961 atggtgaaac acgccattgc agaaggcaaa cgggatgttg gaggaaggag cgaactcttc
     1021 cggagtcatc aggcgcacgt caaactccga gtatctctcc cgcgcgacgc gaaggctctc
     1081 gacagaggga tccactgcga cgactctttt gcatcccagt gtctggaaca aatatggtat
     1141 agaaccgcct attccacagc cgaaatccag cgccgtctcg aacggaagcc aacgaatcag
     1201 tcttcgcagg gaccagtgga agcgttcctc ggcatagaaa tccggtgatt caccagagag
     1261 tgcgcacggg ccctccagaa tctgttcata tttggaagca aactcgtcaa actcggctgg
     1321 gctcataacc ggcctgcctt tagatcgggc agcatagcca acatcgaatc gcgcaaggcc
     1381 tcgcgtgagc ccgcgcgaca ggtccagccg agaccgcgaa tgcggtcggt gttgagcctc
     1441 acgattggaa cgtctccctt ccagccgcga ttaccgccgg tgtattcgaa ctccacctgc
     1501 tcctggttta ggcccacaca ctctaccgcc aactcagcga tctccttcac cgtgatgtaa
     1561 tcgcccgtgg ccacgttata gatagcaaac cgttttgtgg aacgtcgcgc agccaatagc
     1621 acggcctcca tcacgtcccg gacgtggatg tacgacttgc tctgggtgcc gtcgcccaga
     1681 atgtgcagtt tacgcggatc cttcagcagc tgccgcagga aatcgaagcc cactccgtgg
     1741 gtctgccgtg gcccgaccac atttccgaag cggaaggcgc ggcccgtgaa gtcgaacata
     1801 tggcagtagg agcttatcag agcttcgccc gccagcttac tggccccata ggttgagatc
     1861 gggattagcg gaccataatc ttcgtccgcc tccttctccc ccaagtcgcc gtacaccccg
     1921 ctgcccgaag cgtagaggat cgttgcggtc cccgaccggc gcatcgcctc aactacatta
     1981 tttgtgagat aggtgccttc ccggaagtcg atgtcgggct cagtggccgc gcgcgcgatg
     2041 tcgggattgg aagcgagatg gatcacgaca tcgtgtccac tcatggctgc ggtgagctga
     2101 ttcacatcct tcgcatctgc gcgcacgact ttgaaacgcg aa
//
LOCUS       DASRKK010000177         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_989551, whole genome shotgun sequence.
ACCESSION   DASRKK010000177 DASRKK010000000
VERSION     DASRKK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989551"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1756)
                     /locus_tag="VFC15_03920"
     CDS             complement(179..1756)
                     /locus_tag="VFC15_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09338.1"
                     /translation="MHEFIAKHRDKIAGILSGFDRLVFRGTLRSLSFVDGMRHYLGAN
                     DVLLKDFGPHVERVSQQLKSASRGLVQQLNRPLKHLSSSKVSKEKIAREVVAGEGIRE
                     GLICVLTCVEPCWSFEVHRNRETKKLELESRYRKCEFIYQYWMHPEFGFMNARIQTWF
                     PFSIQICVNGRMWLGRQMDRAGIQYAAADNCFPWIEDWTKAQGVMDQQLRTDWPKALE
                     PIAQKLNPAHGEMFKKYPVSYYWSTYQSEWATDIVFQKAAELRRLFPRLVHHGITTFS
                     CTDVMRYLGKPTLLSGEVPRRFSGDVMSDIKQREEGVRLKHTINGNSVKLYDKAFTKP
                     GSVLRAETTIQNGSDFRVYRPKEGDPDGKMSWRPMRRGIADLHRRVEVSRKSTERYLN
                     AFANVDDETTLEELIRKLGQPAYLKGRRVRALRPFADDSELLNAISRGDFMINGFRNR
                     DLQGIFFPSAPTSIEEACRRSAWVSRQLRLLRAHRLITRISGTHRYQLTTSGRKITTA
                     ILSALRATIRQLTPIAA"
BASE COUNT          391 a          605 c          581 g          447 t
ORIGIN      
        1 ccagccgagg atctcatcct ggagaaggcg ctgcagttgt tcaacgagcc tgcggcagca
       61 gcgaaaaagg ccgcgtaaaa gcatcaacca cagaggcgca gaggacacgg agaaagcgtt
      121 ttatctctgt ggcctctgta ctactaacca atagattctt ctcctgacgc gaaggttttc
      181 atgcggcgat gggggtgagc tggcggatgg tggctcgaag ggctgagagg attgcggtgg
      241 tgatcttgcg gccggatgtg gtcagttgat atcggtgggt tccggagatc ctggtaatca
      301 agcgatgtgc acgaaggagt cgaagctggc ggctgaccca tgctgatcgg cggcaagctt
      361 cctcgatgga cgtgggggcc gagggaaaga agatgccctg caggtcgcgg ttgcggaacc
      421 cgttgatcat gaaatcacca cggctgatgg cattgagcag ttcgctgtca tcggcaaacg
      481 gacgaagggc acggaccctg cggcccttca agtacgcggg ttgtccgagc ttccggatca
      541 actcttccag cgtggtctcg tcgtcgacgt tggcgaaggc attgagatag cgctcggttg
      601 acttgcggga gacctcgact cttcgatgaa ggtcggcgat cccgcgacgc atgggccgcc
      661 agctcatttt tccatcgggg tcaccttcct tgggccgata gacccggaag tcgctgccat
      721 tctggatggt ggtctcggcc cgaagcacac tgcccggttt ggtgaaggcc ttgtcgtaca
      781 gcttcaccga gttgccgttg atcgtgtgtt tgagccgcac accttcttcc cgctgtttga
      841 tgtcgctcat cacgtctccc gagaaccttc tgggcacctc gccactcaac agagttggtt
      901 tccccagata gcgcatcacg tccgtacagg aaaacgtggt gatgccatgg tggaccagcc
      961 gcggaaacag ccgtcgcaac tccgctgcct tctgaaacac gatgtcggtc gcccattcgc
     1021 tttgatacgt cgaccagtag taagagacgg gatacttctt gaacatctca ccgtgagcag
     1081 ggtttagctt ctgggcaatc ggttccaggg ccttcggcca atcggttctg agctgctggt
     1141 ccatcactcc ctgagccttg gtccagtctt cgatccaggg gaagcagttg tctgccgcgg
     1201 cgtactggat ccctgcccgg tccatttgcc gtcccagcca catgcggcca ttcacgcaga
     1261 tctgaatgga aaacggaaac caggtctgaa tccgcgcgtt catgaagccg aactccggat
     1321 gcatccagta ctggtagatg aactcgcact tccggtatcg cgactccaac tcaagcttct
     1381 tggtctcccg attgcggtga acctcaaaac tccagcaggg ctccacgcaa gtcaacacac
     1441 agatcagacc ctcgcggatg ccctccccgg ccacgacttc tcgggcaatc ttctccttgc
     1501 ttaccttact tgagctcaga tgcttgagcg gccggttcag ctgctgcacc agaccccgcg
     1561 aagcactctt caactgttgg ctcacccgct ccacgtgcgg accgaagtcc ttcagcaata
     1621 catcgttggc accaaggtag tgccgcatgc cgtccacgaa gctcagcgac cgcaatgtcc
     1681 cgcgaaaaac cagacggtcg aaccccgaca atatccccgc gatcttgtcc cggtgcttcg
     1741 cgataaactc gtgcatggcg cttcttcctt tcctggatgc tttgcctttg tcagctcaac
     1801 atcctacgag aggttcgaag cgcttttact tttggttgcg gctacgccgc gctgtgcctc
     1861 tgtggttgct gtctctccaa ccacaatcga gatcgcgtga cgctacggcg gtggaattgt
     1921 ggccgtagcc ggcggcggaa ccaccttcgg cccctcatcc acattcggcg cgggggactc
     1981 cgcgctttcg ccggatcggg cggaggcatc ctgcgcgctt tgcg
//
LOCUS       DASRKK010000178         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1380407, whole genome shotgun sequence.
ACCESSION   DASRKK010000178 DASRKK010000000
VERSION     DASRKK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380407"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1546
                     /locus_tag="VFC15_03925"
     CDS             113..1546
                     /locus_tag="VFC15_03925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZM09339.1"
                     /translation="MFDRRSKLLSSVTLLHDLGMTCVSFVVAYLLRSLLVHLDFFSRH
                     LPGIYPFSHYLSLLAAFLITWAAVGYFSSFYRDLELSNPIQLILNIVSQLGVVLVVIY
                     AGLYLFRRADVSRTYVLLIGAVDFVLLVIGRAATYSGVSWMRDRLKRYHYLLIVGCGP
                     RAREMAALIEESRGMGLRLIGFVDPNSGNASLENVGGYRVHRIEELAGILQQEVVDEI
                     VFAVNMQELARLEPVMQHCAEVGMRTRVQLEFLPAAYSRIYLEKFRDVLLLSLSSAPD
                     SELRLFFKRIFDVALSFVALIAFSPVLLCIAAMIRVTSPGPVLFRQTRCGLGGRRFML
                     YKFRSMINNAEQMRAELHQLNELDGPVFKISDDPRITTVGRWLRRFSLDELPQLWNIL
                     RGDMSFVGPRPAVPEEVEQYEDWQRRRLRMRPGLTCTWVLEGRNHVDFNRWMQLDLTY
                     IDNWSLWLDFKIFLRTIPIVLSGRGAY"
     gene            1769..>2386
                     /locus_tag="VFC15_03930"
     CDS             1769..>2386
                     /locus_tag="VFC15_03930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM09340.1"
                     /translation="MVSVTIVTWNSAQYLDACFASIEQQDYRELEVIIVDNASTDGTR
                     ERLRQVESRWRVIYNERNVGFAAGQNQAIRAAGGDWVLCLNPDVVLSPDFVTQLVAGG
                     KAHPEAGSVCGKLLRWDPVAEQHRSNIIDSTGIYFTRNMRHLDRGAEEVDRGQYDRPQ
                     FVFGASGAAVLFRRDFIQDVSVEGEVFDEDFFAFREDGDLAWRAQV"
BASE COUNT          429 a          670 c          718 g          569 t
ORIGIN      
        1 gtcctcacgt cagtggttcc attggtttct gcgccaactt cgctgacgtc accgtgcggt
       61 atgaaccgaa ttcccaggtc ataacgtcca actgtaacca acggcagtta ctgtgttcga
      121 tcgtcgttcc aagctcttaa gttcggtcac cttgctgcac gatctgggga tgacgtgtgt
      181 ctcctttgtg gtcgcgtatc tgctgcgcag tctgctggtc cacttggact ttttcagccg
      241 gcatttgccg ggcatttatc ccttcagtca ttatctgtcg ttgctggccg cctttctgat
      301 tacgtgggcg gcggtcggat atttttccag cttctaccgc gatctcgaac tcagtaaccc
      361 gatccagctc attctgaata tcgtcagcca actcggcgtt gtgctggtgg ttatttacgc
      421 cggtttgtat ctgtttcgtc gtgcggacgt gagccggacc tatgtcctgc tgatcggcgc
      481 ggtggatttc gtcctgctcg tgataggccg cgcggcgaca tactcgggcg tttcctggat
      541 gcgcgaccgg ctcaagcgct accactacct gctgatcgtc ggttgtggtc cgcgcgcccg
      601 cgaaatggct gcgctgatcg aggagagccg gggcatggga ctgcggctga tcggctttgt
      661 cgatcccaac tcggggaatg cttcgctcga aaacgtgggc ggctacagag tccatcgcat
      721 cgaggagttg gccggtattc tgcaacaaga agtggtggat gagattgtct ttgccgtgaa
      781 catgcaggag ctggcccggc tggagccggt catgcagcat tgcgccgagg tgggaatgag
      841 gacgcgggtg caactggaat ttttgccggc cgcctattcg cggatctatt tagagaagtt
      901 tcgcgacgtg ctactgctca gtctgtcgag cgcgcccgat agtgagttgc gactcttttt
      961 caagcgtatc ttcgatgtcg cgctctcctt cgtagcgttg attgcgttct cgccagtgtt
     1021 gctctgcatc gcggccatga tcagggtgac ctcgcccggc cctgtgctgt ttcgccaaac
     1081 ccgctgcggc ttgggcgggc gccgcttcat gttgtacaag ttccgttcca tgatcaacaa
     1141 cgccgagcaa atgcgcgccg agcttcacca gttgaacgag cttgacggcc cagtgttcaa
     1201 gatcagcgac gacccccgca tcaccaccgt gggacgctgg ctgcgccgct tcagccttga
     1261 cgagttgccg cagctttgga acattctgcg gggtgacatg tccttcgtgg gaccgcgtcc
     1321 cgccgttccc gaggaggtcg agcagtatga agattggcag cggcgtcgtc tgcgcatgcg
     1381 gcccggcctg acttgcacct gggtgttgga aggccgcaac catgtggact tcaaccgctg
     1441 gatgcaactc gatctcacgt acatcgataa ctggtcgctg tggctcgatt tcaagatctt
     1501 tctgcgcacc attcccatcg ttctttccgg acgcggtgca tactagaggc tgtacgaatt
     1561 cctgtgtccg ggtagagccg gccttcaggg atcgtgtcgc gacctcgtat cggtttgcag
     1621 cagtggaatg aagcacacaa gaccagcccc gaatggggcg aaagaactta gcccagcgct
     1681 tcagcgctgg gtacagtggc aaacaaagcc gagtcccgta gggacggcag agttctcaca
     1741 caccctttga aggcccgcac taccactaat ggtctcagtc acaatcgtca cctggaactc
     1801 ggcacaatat ctcgatgcgt gtttcgcctc gatcgagcag caggactatc gcgaactcga
     1861 ggtgatcatc gtggacaacg cttcgaccga tggcacgcgc gaacggttgc gtcaggtcga
     1921 gtcacgatgg cgagtgattt acaacgagcg caatgtcgga tttgctgccg gacagaacca
     1981 ggcgatccgg gcggcaggcg gagattgggt gctctgtctt aatcctgatg tcgtgctcag
     2041 tcccgacttt gtgacgcagc tggtggctgg aggaaaagcg catcccgagg cgggctcggt
     2101 ctgcggaaaa ttgttgcgct gggacccggt cgcggaacaa caccgaagca acattatcga
     2161 ttccacggga atttatttca cccgcaacat gcgccatctc gatcgtgggg ccgaggaggt
     2221 tgaccgcggc cagtatgatc gtccgcagtt cgttttcggc gccagcggcg ccgcagtgct
     2281 gtttcgccgc gacttcatcc aggatgtttc ggtggagggc gaagttttcg acgaggactt
     2341 cttcgctttt cgggaagatg gcgacctggc ctggcgtgca caggtg
//
LOCUS       DASRKK010000179         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1282992, whole genome shotgun sequence.
ACCESSION   DASRKK010000179 DASRKK010000000
VERSION     DASRKK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282992"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(989..1774)
                     /locus_tag="VFC15_03935"
     CDS             complement(989..1774)
                     /locus_tag="VFC15_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM09341.1"
                     /translation="MPRIRVIVACSNLITGRLLARLLQTRPQFRVLGSVSNSRNLLNS
                     LERTRADVVLVNADLPGGSYGVFPQLREMSRQYPDLPWVLLVDRSDPPLVLEAFRAGA
                     SGVFSCANSNTRLLIKCISRVAEGQIWAEATHLKFLLAAWRGQSLPASSSRGGPLSLL
                     TAREESVVRLVAQGMSNREIAGHLGLSEHTVKNTMFRLFEKLGFSNRVELVLYAIAKL
                     NQAAFPDAGPPSPAVPNENSVQPMANVIPPTERRSSGSSRTCP"
     gene            complement(2516..3703)
                     /locus_tag="VFC15_03940"
     CDS             complement(2516..3703)
                     /locus_tag="VFC15_03940"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HZM09342.1"
                     /translation="MPWKVSSVVDQRKGFVDDYNRGWWTMAELCRRYQISRETGYRRV
                     ERCQREGEAGLQDRSRAPLEHPNQTAAAIEQQVLELRRRHPTWGARKLRSYLGMQRPA
                     TLWPAPSTIGELLRREGLTVPRRQRRRAPPYTQPFEAADAANQVWCADFKGWFRTGDG
                     ERIDPLTMTDAYSRYLLRCQAVEKTDTERVRAIFEAAFREYGMPVAMRTDNGAPFASR
                     AIAGLSRLAVYLMKLGIVPERIAPGHPEQNGRHERMHRTLQADTASPPQAHRRAQQRA
                     FDHFKWDYNEERPHEALGMKTPASCYTVSPRAYPARVPEPEYDSGLQVRRVRERGAFS
                     WQHENVFLSETLIGERVGLQPIDDRYWLVQFAAFPIACFDSQQLIIEPLPDEDGDDGD
                     DHD"
BASE COUNT          873 a         1208 c         1089 g          909 t
ORIGIN      
        1 atcaacgcaa acaacctccg aacctgcttg tccgtgacca atccgcctcc gctccgatga
       61 accggaatac gcttggccta attgtcgttg gatcgggaat tttgtttgtc gtcgttcggt
      121 acttctaatt gtcgcccgtc agttcagcac cagagtgctt tcttcgaact gttttggcac
      181 gaactgcttc ttcgcagcgg ctacgaagtc gaagtacatc cccctctaca tgacgtagcg
      241 acaaaccccg atttcttggc aaagcggggc ggaattggga tcagttggat tgcaagctaa
      301 tttttgtcgg attccacggg ttgacacctt cggtcggctg agctcctctt acccatttcc
      361 cgtagcgaag aagcctcgcg cactgtctga cttggtcgcc gcaaggcata accaatttga
      421 acggcccggc ggggacgatg gtcgcctggt tcaagcacct ggtgtaatgc caggtggtcg
      481 ggtgtcagcc acacccctaa tgtgcgactg ttttcaacga gttcgggaca ctttgggcct
      541 gatcggcgtt gtgctgtagt caaatctgta actggaaatt cctcaaggat ctacagcgtt
      601 caacaatccc caaacttcaa cctactgaat ctaaggaaga taagatgtat accaaaaagg
      661 taactcacct aatcccgaac cagattccaa agcctattga accttgtgga aattgatggt
      721 ttgtaagtga ttgatatgga acctgttgca agccgctcgt tgaaatgtgt tgaagtttga
      781 ggttctttga ggctttgaca gttacaattt cgactacagt cgcgcctcta aggtgaaccc
      841 cctccactga gacagtttcc cgttgctaca ctcgcagttg ctcttgattt gaaccttcac
      901 ttgaaacaat ccgacctttg tacaagttcg caggtcggac ggaatattag cgcaccacag
      961 gaacatttgg ttccggatgg attttctatt agggacaggt ccgcgaactg cctgagcttc
     1021 gccgttcggt tggaggaatg acattcgcca tcggctgcac agaattttcg ttagggacag
     1081 ccggactagg agggccggcg tcggggaagg cggcctggtt cagctttgcg atggcataga
     1141 gcaccaactc cacgcgattt gagaagccaa gcttctcaaa caaacgaaac atagtgttct
     1201 tcacagtatg ttcgctgaga ccaaggtgac ccgcgatctc ccgattcgac ataccctgag
     1261 ccaccaaacg aaccacgctc tcctcccgcg ccgtcagcag cgaaagcggt ccccctcggg
     1321 acgaacttgc aggcagggat tggccgcgcc aagccgcgag aagaaacttc aggtgtgttg
     1381 cctcagccca tatctgtcct tctgccacgc ggctaatgca ctttatcaac agccgcgtgt
     1441 tcgagttcgc gcaggagaat accccagaag cgccggcccg aaatgcctcc agaaccaacg
     1501 gagggtcact ccggtcgacg aggagcaccc aaggcaaatc gggatattgc cggctcatct
     1561 cccgcagctg agggaaaacg ccatacgagc cccccggtag atcagcattg accaagacca
     1621 catcggcccg ggtgcgttcc agcgaattta acaagttgcg actattgctg accgaaccta
     1681 agactctgaa ttgcggtctt gtctgcaaca atcgcgctag caaacggcca gttattaagt
     1741 tagaacatgc gacaatgacg cgaatgcggg gcattcagtg tccattctgt tacaggattt
     1801 ctcatcagat tgatcacaac taactgccat gttgcggcag cgattcttgc gggaacggga
     1861 ccttctgcac ataagggacg aatggccatg tctgcaacag gtaatgcgat tagaaggctt
     1921 tcccgttaca tccctgtctt tatattgatc tgaaacaacg aagtataaca ccttcaatgc
     1981 acagaagata aagttaattt ttatagcaca tgggggtaga tgcccactcc actttacggg
     2041 agtgcaccta ctagccaaaa catgccacga ccgtcgctcg tgagtaagcc tattagggat
     2101 agcttatcca aaataccgca aaggtctgca cgtcgaccca ttagttttgg aagggaattg
     2161 ctgcactatc ttaagacctt gcttactgca tcactttgca tgtacgaaat tcgcggctgg
     2221 cagcaccaca cagctcggaa ggcgagcaaa gaaaacgacg accggatgtg tgcggccatt
     2281 gttttgtaca gcccggcaca tagttgacag tttagaccgg gcacatagtt gacgcttttg
     2341 gcccgggggt ttgggttttc tcccccgcag ggggaggata aataaatagc cgtcgtgggg
     2401 gtggaaatgt gggaatcgcg ccgcctttgc gcgatttcca agggacggtg ggaagcgcgg
     2461 gaaagccgtt tttgcttttc cgcgatttcc accgtcccgg catttccacc cccggtcagt
     2521 catggtcgtc cccgtcgtcc ccgtcctcgt cgggcaaagg ctcgatgatg agttgctggc
     2581 tgtcgaagca ggcaatcgga aaggcggcaa actgtaccag ccagtagcga tcgtcgattg
     2641 gctgcaggcc cacacgctct ccgatcaagg tctcgctcaa aaatacgttc tcatgctgcc
     2701 aggaaaatgc gccacgttcc cgcacccgtc gcacctgcag gccgctgtcg tactccggtt
     2761 ccggcacccg cgccggatac gctcgcggcg aaaccgtgta gcatgacgcc ggcgttttca
     2821 tacctaacgc ttcgtgcgga cgttcttcgt tgtagtccca cttgaaatgg tcgaaggccc
     2881 gctgctgggc ccgccgatgg gcctgcggtg gcgacgccgt atccgcctgc aaggtgcgat
     2941 gcatgcgctc gtgccgccca ttctgttccg gatgtccggg cgcgatccgc tccggcacaa
     3001 tccccaactt catcaggtac accgccagcc gcgacagccc ggcaatggcc cgcgaggcaa
     3061 acggtgcccc gttgtcggtg cgcatcgcca ccggcatgcc gtactcgcga aacgcagcct
     3121 cgaagatggc ccgcacccgc tccgtgtcag tcttctccac cgcctggcag cgcaacaaat
     3181 agcggctgta agcatccgtc atggtcagcg ggtcgatgcg ctctccgtcg ccggtgcgaa
     3241 accagccctt gaagtcggca caccacacct ggttggctgc gtcggccgct tcaaacggct
     3301 gcgtgtaggg cggcgctctt cgccgctggc gccgcggaac cgttaaccct tcgcgccgca
     3361 gcagttcgcc aatggtgctg ggcgccggcc acagcgtcgc tgggcgctgc attcctaagt
     3421 acgagcgcag cttgcgcgcg ccccatgtgg gatgccgccg ccgcagctct agcacctgtt
     3481 gctcgatcgc cgccgccgtc tggttgggat gctccagcgg cgcccgactg cggtcctgca
     3541 acccggcttc cccttcccgc tgacagcgct ccacccgccg gtagccggtt tcgcgcgaga
     3601 tctgataacg acggcacaac tccgccatcg tccaccagcc gcggttgtag tcgtcgacaa
     3661 aacctttgcg ttggtccacc acactgctca ccttccaggg catgcctccc ttctacatgc
     3721 ccccgctgtc cggaccaaac tgtcaactat ctccccggtc tgttctgtca accatgtccc
     3781 cggtctgtac cagagcttag cccagcgctt cagcgctggg tagagtgggg aaataagacc
     3841 gagtcccttc agggacggca cagtcctcac acgacctcct cagtatgggc acttggaaat
     3901 gactttgggt cgcgctttcc tggtcgcccc tttattgcat tttagtatgg tattttggta
     3961 cattgttacc atgacaaaac atggtggcct cgtcgtcgaa cgcgtccaga ccggcgtccg
     4021 tatggaggga cgcctgctca aggtgctgaa gggactggcc gagctgaaag accttacgc
//
LOCUS       DASRKK010000180         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_12497, whole genome shotgun sequence.
ACCESSION   DASRKK010000180 DASRKK010000000
VERSION     DASRKK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12497"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1586
                     /locus_tag="VFC15_03945"
     CDS             153..1586
                     /locus_tag="VFC15_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM09343.1"
                     /translation="MPEATITRPAAGREETVSLGSVLIIDDEAAIRESLQTLLELEGY
                     EVETASDGTEGLARLADRPYDLVLLDFAMPERNGIEILQDIRERDSELAIIMITAYGT
                     VENAVNAMQAGATNFIQKPWDNEKLLADVGAAVGRRRAEAEVVQLKRALKQRYNFEHI
                     IGKSEPMLRIFDLVAQVAPSRSTVLLQGESGTGKELIAKAVHMNSTRKDFAFVPVNTG
                     SMPADLLESTLFGHVKGAFTSAIASKKGLFEMADRGTLFLDEIGTMSMDTQAKILRVL
                     QDRKFMHLGGVSEIQVDVRIIAATNVDLRQQVKDGKFREDLFYRLNVITIDLPPLRQR
                     KNDIPLLAQHFAQKFAEENGKPQLLISAEAMRPLMDYDWPGNVRELENVIERAVVLAN
                     GPSLTVDLLPDNMVGRGSKLAMMDHRPDASLFEIMEDCERRIISDMLEKSGWNQTEAA
                     DRFRIPLSTLNQKIKRLNIEIKKKGRE"
     gene            complement(1712..2818)
                     /locus_tag="VFC15_03950"
     CDS             complement(1712..2818)
                     /locus_tag="VFC15_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HZM09344.1"
                     /translation="MNPYHFVRKFVSSAITGAILFCGALQSAQACTGIRLIAADGTVV
                     HARTLEFGIDLKSNVIMVPRGYARVGTTPDGKEGLKWKAKYASLGANGVGLPFIFDGL
                     NEKGLAVGTFYFPTSAGYMPYSASVSSKTIAPWEVGSWLLENFASVEEVRENIGKVVV
                     PEVVFKEWGFAPPVHYIVQDASGKGIVIEYFGGKLNIFENPLGVITNSPTYDWHMTNL
                     RNYVNFSMSNVAPVKLGSVTLQPTGQGSGMLGIPGDFTPPSRFVRAVAYSQSVLPSKT
                     GDDAVLEAFHILNNFDIPKGAARDHEKDSHGNIEADYTLWTSVNDLKRTRFYFRTYDN
                     SQIRMVDLTKMNLDAKDIVSIPMTGNEVIKSLTP"
     gene            complement(3070..3384)
                     /locus_tag="VFC15_03955"
     CDS             complement(3070..3384)
                     /locus_tag="VFC15_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09345.1"
                     /translation="MSCPICSYLKPNGVRCGSPAQHGHNYCYFHTREHRTAMYGARAR
                     RRYSTVRFDLPPLDDMNVIQDLLSQLAGALASDTIDYRRASAMLTALRLASFELRNPQ
                     EW"
BASE COUNT          863 a         1073 c         1194 g          757 t
ORIGIN      
        1 ggcgaagagt ggccacagtg gaggaactgg cctgggactg tcggtgacct acggaatcat
       61 tcaggaacac gcgggtaaga tccacgttga gagttcgccg gatcagggca ccaccttcac
      121 catggaattt cccatggtaa gaaaggtcgt caatgcctga agccacgatt acccggcccg
      181 ctgccgggcg ggaggaaacc gtttctcttg gttcggtgct catcattgac gacgaggcgg
      241 ccatccgcga gtcgctgcaa actctgctgg aactcgaagg ctacgaggtc gaaaccgcta
      301 gcgatggcac ggaaggattg gcgcgtttgg ccgaccgtcc ctacgacctc gtgttgctcg
      361 actttgccat gccggagcgc aatggcatag agattctgca ggatatccgc gagcgcgact
      421 ccgaactggc gatcatcatg atcaccgcgt acggcacggt ggagaacgcg gtgaacgcca
      481 tgcaggctgg ggcgaccaac ttcatccaga agccctggga caacgagaag ctactggccg
      541 acgtgggcgc ggccgtgggg cgtcgccggg ctgaagccga ggtcgtccag ctcaagcgcg
      601 cactcaagca gcgctacaac ttcgagcaca tcatcggcaa gagcgagccc atgctgcgca
      661 tctttgatct ggtggcgcaa gtcgcgccca gccgctcgac ggtgctgttg caaggcgaaa
      721 gcggaactgg caaagagctg atcgccaaag cggtccacat gaactcgacg cgcaaggact
      781 tcgcgttcgt tcccgtgaac accggttcca tgcccgcaga cctgctggag tcaacgctct
      841 tcgggcacgt gaaaggcgca ttcaccagcg ccatcgccag caagaaggga ctgttcgaaa
      901 tggccgatcg gggaactctg ttcctcgacg agatcggcac catgagcatg gacacgcagg
      961 ccaagatcct gcgcgtcttg caggaccgca agttcatgca cctgggcggc gtcagcgaaa
     1021 tccaggtgga cgtgcgcatc attgccgcca ccaacgtcga tttgcgccag caggtgaagg
     1081 acggcaagtt tcgcgaagac ctcttctacc gtttgaacgt gatcaccatc gaccttccgc
     1141 ccttgcggca gcgcaagaac gatattccgc tactggcgca gcacttcgcg cagaagttcg
     1201 ccgaggaaaa cggcaagccg cagttgctga tatcagccga ggccatgcgt cctctgatgg
     1261 actatgactg gccgggcaac gttcgcgaac tggaaaacgt gatcgagcgc gccgtggtgc
     1321 tagccaacgg accaagcctc actgttgatc tgctgcccga caacatggtg ggccgcggca
     1381 gcaagcttgc catgatggac catcgtccgg atgcgtcgct gttcgagatc atggaagact
     1441 gcgaacgccg catcatcagt gacatgctgg aaaagagcgg ctggaaccag accgaagccg
     1501 ccgaccgctt ccggattccg ctctccacgc taaaccagaa gatcaagcgc ctcaacatcg
     1561 agatcaagaa aaagggtcgc gagtagttag tagttaccgg ttcctgctcc gcgcctaatg
     1621 gcgcggcctt tttttggcga ggtgcatcga gttagcgccg cgctttgggc gctaactcga
     1681 gtacacaacg ctccgcacag cgcgtctcag gttacggggt caaggatttg atgacctcat
     1741 tgcccgtcat gggaatgctg acgatgtcct tggcatcgag attcatcttg gtcaggtcca
     1801 ccatacggat ctggctgttg tcgtaggtac gaaagtagaa gcgcgtgcgc ttgagatcgt
     1861 tgacgctggt ccagagcgtg taatcggcct cgatattgcc gtgcgaatct ttttcgtgat
     1921 cgcgggccgc tcccttgggg atatcaaaat tgttgagaat gtggaacgcc tcaagaaccg
     1981 cgtcatcccc ggtcttggac ggcagcaccg attggctgta cgcgaccgcc cgcacaaagc
     2041 gcgaaggcgg agtgaaatct ccgggtattc cgagcattcc cgaaccctgg cccgtgggct
     2101 gcagcgtgac cgatcccagc ttcacgggtg caacattgga catcgagaaa ttcacataat
     2161 tgcgcaggtt ggtcatgtgc caatcgtacg ttggcgaatt ggtgatcacg cccagagggt
     2221 tttcgaaaat gttgagcttt ccgccgaagt actcaatgac aatgcccttg cccgaggcgt
     2281 cctgcacgat gtagtgaacc ggcggggcga atccccactc cttgaaaacg acttccggca
     2341 cgacaacttt gccgatgttt tcccggacct cttcgacgct ggcgaagttc tccaacaacc
     2401 aagacccgac ttcccacggg gcgattgtct tgctcgaaac gcttgccgag taaggcatat
     2461 atccggcgga ggttgggaag tagaacgtgc cgacagccaa gcctttctca ttcagcccgt
     2521 cgaaaatgaa gggcagcccg accccattgg ctcccaggct ggcgtacttt gccttccatt
     2581 tcagaccttc cttgccgtca ggagttgtgc ctacgcgagc atagccacgc ggaaccatga
     2641 tcacgttgga cttgaggtcg ataccgaatt ccaaggtgcg agcgtggacg acggtgccgt
     2701 cggctgcgat gaggcggatg ccggtgcaag cctgggccga ctgtagggct ccacaaaaca
     2761 agatggcgcc ggtaattgcg gatgatacga acttgcggac aaagtgatac ggattcacgg
     2821 ttgaagtcct ttcggtctgg ttggggggat gattgcaatc ctacgccacg agtgcttgca
     2881 agcgagccag agccaacatc cagacgggac aaccggcttt ggcggcccac agtggttcat
     2941 tcgaaaacat ttcacttctg tcaaaattcc tcattttcat agggataggg ggaatttacg
     3001 cgcaaaatcc ttgctgtcaa tgacttgcac gtaaaatatt cattcgcaaa gagttgcatg
     3061 gccgtatttt caccactcct gcgggttgcg aagctcgaag gaggccaagc gcagggcggt
     3121 gagcatcgcg gaggcccgcc ggtaatcgat agtgtccgac gcgagagcgc ctgccagttg
     3181 cgagagcaag tcctggatga cgttcatgtc gtcgagcggg ggcaggtcga agcggacggt
     3241 ggagtagcga cggcgggcgc gggccccgta catggcggtg cgatgttctc gggtgtggaa
     3301 gtaacagtaa ttgtggccgt gctgggcggg tgagccgcaa cggacgccat taggcttgag
     3361 atagctgcaa atgggacaag acatgggcgg ctccgagaga gcaagaagta gcaagtaata
     3421 agtagtaagc aataagcgaa gaacaaaaga gcaagagaca aagagcgact agcgacatcg
     3481 gcgcgcgccg tctggcgagc ggagcggcga agccgtacag cgtctttgtg gggcattgcc
     3541 ccatggtagg agggtggcag cggagcgggg gttggtcaag ttacgaaggt accgggtggg
     3601 gatgaacttt cgtaaccaaa agcagtttcg agtctcaagt ttcgagtttc gagaagcact
     3661 gcggagcgaa cgcgcgggaa aaggacgaaa ttcgctcggg ctgaagcctt cgcggaagcg
     3721 tgccgtcccc tacgggactc ttcattcata aaacgagcct acccagcact ccgctgcgct
     3781 gcgtgctggg ctatcttctg ccgtcccgcg aggcgggact ggattgtcac gggttcgggc
     3841 tcaactgatg tccccggtct gagtagccca ccgtgtcgct gtgcgac
//
LOCUS       DASRKK010000181         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_696697, whole genome shotgun sequence.
ACCESSION   DASRKK010000181 DASRKK010000000
VERSION     DASRKK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696697"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..721
                     /locus_tag="VFC15_03960"
     CDS             485..721
                     /locus_tag="VFC15_03960"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZM09346.1"
                     /translation="MPKAKESTPRNTTAKTSSSTKGMGTVGKVMHEYKLGTLKSGKGG
                     TGGKVKSRKQAVAIGLSEARKAGAKIPHKGEKSS"
     gene            complement(840..>2026)
                     /locus_tag="VFC15_03965"
     CDS             complement(840..>2026)
                     /locus_tag="VFC15_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM09347.1"
                     /translation="KSDSVTTRFGVDPDVLRLGLVSFLTDVSSEMIFSVFAIFFTTIA
                     GASSALLGLVEGFADFSASSLDYVAGWWSDKTGRRKAFALVGYGFSTVAKIILLVASS
                     VVALSCFRIIERLGKSFRGPPRDAWLSAIAAKDTRGYSFGVHKALDKAGAVLGPLAAY
                     GLLSWHGETASSFRMLFWVAFVPAVLSVFVLGLINDPPRVQSKRESIFVAWKTLSPGF
                     KRYLVAAGIFALAYFSFGFLLLRANSAGFSVKDVVLLYALFNAAFVIAAPLVGKLGDR
                     IGRTRIVVLSYLIYLVMSLGFAFAMAKWQILLLFVIYGVFYSIDEAQSKAFIADVEPE
                     RRATAIGVYNFVTGVIYLPASLIAGALWAVHPSIVFIVAAFLSLAAITAFVWLRPADN
                     ST"
BASE COUNT          508 a          579 c          594 g          345 t
ORIGIN      
        1 gtagccgggt tgtcaggctg gccggattga tcaggtgtgg tggttttgtt gtccattgct
       61 tttgagattc actagaagcg cggctcacag tgctccaagg tgtcttgcct ccgaatatct
      121 cgctgcaatt ctcgggccat cgctggaacc ctgtctgttc tcatgcggta ccacgggatg
      181 gctgtcatga agagagggga ttgcaagcga agcttctacg gcggcgcagc cgctccggtc
      241 gcccgccggc tccggtagtc gatgcgtccc gttgacatga cgttccgagt gacgtgcagg
      301 cattctcgag cctcctcaat ccgcatactg cgaaacaccc gattgtcggg cggcgaaccg
      361 aaccggcagc aatcgtttga aactcatcgc gctcctgtcg gcacgaaagg agtatgtccg
      421 cagacgtccg ctagtgaatc gcgacgatga gctacactgc gattggagga caggaggaca
      481 ctatatgcct aaagcgaaag agagcacccc cagaaacacc accgcgaaga cgagttcgtc
      541 gaccaaaggc atgggcacag tcggaaaggt catgcacgaa tacaaactcg gcacgttgaa
      601 atccggcaag ggtggcacag gtgggaaggt gaagagccgc aaacaggcgg tggccatcgg
      661 tctcagcgag gcgcggaagg ccggggcgaa gatcccgcac aaaggagaga agagttcttg
      721 atcgcaaagt cggcgctctg cgcgcgcatc tcgcaggaag ccccaggcga ggcgcccgta
      781 actcccggag aagctgattg ccggcgcaaa actgtctcaa tccaccaggc gaaatgccgt
      841 cacgtgctgt tgtcggcagg ccgcaaccac acgaatgcag tgattgccgc aagcgagagg
      901 aaggcagcta cgatgaagac aatgcttggg tgcactgccc acaacgcccc tgcaatcagc
      961 gaggcaggta ggtagatcac tcctgtcacg aaattgtaga caccgattgc cgtcgcgcgc
     1021 cgttccggct cgacatccgc gatgaacgcc ttgctctgcg cttcgtcgat cgagtagaag
     1081 acgccataga taacgaatag gaggaggatc tgccatttcg ccatcgcgaa agcgaacccg
     1141 aggctcatga caagatagat caggtagctc agtacgacaa tgcgcgtcct gccgatccgg
     1201 tcgccgagtt tgccgacgag tggtgcggcg atgacaaaag cggcattgaa caacgcgtac
     1261 agcagcacga cgtctttgac cgagaaaccg gcactattcg cacgcaggag caggaagccg
     1321 aaactgaaat aggccagtgc gaaaatcccc gccgcgacaa ggtagcgttt gaacccgggg
     1381 ctcaacgtct tccatgcgac gaagatactt tcgcgtttgg actgaacgcg cggtggatca
     1441 ttgatcaagc caagcacgaa aacactgagc acggcaggaa caaacgcgac ccagaatagc
     1501 atccggaacg atgaagccgt ttctccgtgc cacgaaagca atccataggc cgcaagcgga
     1561 ccgagaacag cccctgcctt gtccagcgcc ttgtgtacgc cgaacgaata cccccgggtg
     1621 tctttggcgg cgatagcaga cagccacgcg tctcgtggcg gaccgcggaa gctcttgccc
     1681 aggcgctcga tgatacggaa gcaactcagg gcaaccaccg aactggcgac caataggatg
     1741 attttggcga ccgtcgagaa cccatatcct acgagagcaa aggccttgcg tctgccggtc
     1801 ttgtccgacc accaaccggc tacataatcc aaagatgatg cggagaagtc ggcgaaaccc
     1861 tcgacaagac ccagcaaagc gctcgacgcc cctgcgatgg tcgtgaagaa gatcgcgaaa
     1921 acggaaaata tcatctcgga actgacatcg gtgaggaagc tcacgagacc gagcctgagc
     1981 acgtcgggat cgacgccaaa cctcgtcgtc acagaatcag acttta
//
LOCUS       DASRKK010000182         7051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_989863, whole genome shotgun sequence.
ACCESSION   DASRKK010000182 DASRKK010000000
VERSION     DASRKK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989863"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /locus_tag="VFC15_03970"
     CDS             complement(<1..2194)
                     /locus_tag="VFC15_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM09348.1"
                     /translation="MNRLGSWLLLTLCIVSLLAVPLMAQKTSGTIRGVVTDPSGAVLA
                     NVPVDITNSETGQTRTVNTNTQGEYVAPEIAVGTYTVTVKAPNFKESVSKNVDLHTSS
                     TQVLNIQLQVGSTSEQVTVAASEVQVQTDNAALGEVVTGEQVRELPLNGRSFVQLTQL
                     EPGVSAANNFSANNKGLLAGVDFSVNGNATTNNLFLVDGANNNDVGSNRTILIYPSIE
                     AISEFKMLRNSYGPEYGQASGAVINIVTRSGSNQWHGDVLYFGRNTALNAYEYFAHAN
                     EQLAVAGGAAPSNQFKNVVQRNDFGFSLGGPIKKDKLFVFYSQEWNYEKRGQTRNSCV
                     PTTAEINGDFSGPEFTGAPGHKVDQCNVAQPNIPVALQAPGNPYKFNSISPAGQDILL
                     NFPAPNINGTSGGIPNWATSVTSPIDWRQENARVDYNISHTQTLMGRYTQDSWTNNAP
                     NGNSALGLWGDDPWPVLESNWAQPSKQIVLRLTSTIGTSMVNDLEFAYSDNRINITPG
                     GTDPGLLAQTSAAFPPGYPLALKTQKQGIPTLWGGSIGGYGSGNNLWLIAPWNNSLDI
                     YTVRDDFSKVYNNQTFRFGGFLSWNGKNEDIGATSASEYPQFGAEDWDTNVPTGNGAA
                     DLLVPGAKWGWTELSTNVNAHVRWRDYELYAADNWKVRRNLTLDLGVRYSILAPPFQP
                     SGLETNFNSALYNPALGSSDACNGIVTVPGMNPCAAANAVFGTNFTSGT"
     gene            2699..3772
                     /locus_tag="VFC15_03975"
     CDS             2699..3772
                     /locus_tag="VFC15_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZM09349.1"
                     /translation="MPGHSKPPNNGGTTLKELAKHLGLTKGTISAVLNDSPYAKAIPQ
                     HTKDRIIAAARELNYHPNFFARSLRKKRTFTVGVIAAEIGDAYGSVVIGGIESALSER
                     KYFFVTVVHHHDPQLLHQYSDILLARGVEGFITIDTTVNQTQRVPTIVISGHRQIEGV
                     TNITLDHFQAAHLVLAHLTELGHSEIAIIKGQPLSVDSGARWHGITEAARGLGIQLRT
                     ELTTQLAVDDPSPQEGYKTTKELLSRKVPFTALFAYNDISAVGAIRAIKESGLRVPED
                     ISVVGFDDVREAAYHVPSLTTVRQPLRKIGVMAAETLIDRIEGRDGHEAGILVEPELV
                     VRESSTAVSPTPLHRNSERTRLS"
     gene            complement(3818..5338)
                     /locus_tag="VFC15_03980"
     CDS             complement(3818..5338)
                     /locus_tag="VFC15_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09350.1"
                     /translation="MALAVTFCFAMNLAATTVDQANFSAIETLIKQSKLEPAETQLQD
                     ILQKHSANYRAEDLLGQVQLKQGRYDEAETLFRQAAQHNPKSEEACVSLASLLKDEGR
                     TEEAIAQYEACQKLAPQKAGNASELAMLYQEAGEYEKSLRVASAIPPAARSTKLLPVL
                     AVDYLALGKSEPAHKAVGDVLAHASADPEIVPQLATALVQHGMTNDAAELLKFVQSHQ
                     KNTPAFLAAVAQVQAAEGDQQQARGTVMKALQMDPKSVEALSAAAALAEMANDWDAAA
                     GFLDRALAVGPPRSDLLQQVVYVEIKKHDLVAAHEVAQRWHKLRPNDPASTMALATVM
                     IEANHFGEAKPLLEKFLANARNDKRAQLAMGLADYNTGNLPEASHYLTASLGQGPEDS
                     TARYYLGLIAKQQGDIEAAVDQMNWALAVNPDNPRALGALGQLYLQQNDPEKARPLLE
                     KAVEKVPAEPQNHYELARVYNKLGMKDEAQQQLQLYEKLRNKRPTDASQGQTVTPQ"
     gene            5644..>7051
                     /locus_tag="VFC15_03985"
     CDS             5644..>7051
                     /locus_tag="VFC15_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM09351.1"
                     /translation="MPGSTQPEMKYIVQQMAGGVALFDCDNDGKLDIAVVTPSTIPGY
                     RAGGDLMVALYHQDPGFKFTDITKSAGLTRKGWGMGLAVADYDNDGLPDLYVTGYGGN
                     ALYHNLGNCKFEDVTDRAGVAGGGFSTGAAWGDYDRDGNVDLFVSRYIHTDVATLGEI
                     KTSYKGLVVEAPWGMSGESDFLFHNRGNGTFEEVSRKAGVSDPEGRLGLGVVWGDYDN
                     DGWPDIFVANDTEPSYLYRNKHDGTFEDQALLSGAAVSAEGKAMGSMGVDFGDFDRDG
                     RLDITVATFAYQPDNLFHNAGDEFNDITWAAKLGQPTFRWVKWGTGFVDFDNDGLQDI
                     LVASGHIYTAIDQLPGEPAFREPMILFRNTGQLKFDDVSDTSGVNDEPLQSRRGIAFG
                     DLNNDGNIDAVVFNVNGPPSILINDTRNSNHRVMFRLVGTTSNRAAIGARVTITAGGV
                     TQLREVKGGNSYISQSDLR"
BASE COUNT         1472 a         2043 c         2017 g         1519 t
ORIGIN      
        1 gtgtcccact ggtgaaattg gtcccgaaca ccgcgttagc cgccgcgcag ggattcatgc
       61 caggcacggt cacaattccg ttgcaggcgt cggaagagcc cagtgccgga ttgtaaaggg
      121 cggaattgaa gttggtttcg aggcctgacg gctggaatgg cggcgccaga atcgaatacc
      181 ggactccaag gtcgagagtc aggttccggc gtaccttcca gttgtcagca gcgtacagtt
      241 cataatcgcg ccagcgcacg tgggcgttga cgttggtgga cagttcggtc catccccact
      301 ttgcgcccgg aaccagcagg tcagcagcgc cattaccagt gggaacgttg gtgtcccagt
      361 cctcagctcc aaactgcgga tattcactgg cgctggtcgc accaatatcc tcgttcttac
      421 cattccagct aaggaaaccg ccgaagcgga aagtctgatt gttatagacc ttggagaagt
      481 cgtcgcgcac ggtgtagatg tcgagactgt tgttccaggg agcgatcaac cacaagttgt
      541 tgccgctacc gtaaccaccg atgcttccgc cccagagagt cgggatgccc tgcttctggg
      601 tcttcagcgc cagcggatag ccgggaggaa aggctgccga ggtttgcgcc agcagcccag
      661 gatcggtgcc gcccggtgtg atgttaatgc ggttgtctga gtaggcgaac tccaaatcgt
      721 tcaccatcga agtcccgatg gttgaagtta accgaagcac aatctgcttc gacggctgcg
      781 cccagttgct ctccagtact ggccagggat cgtctcccca tagacccagt gccgaattgc
      841 cgttaggagc gttgttggtc caactgtcct gggtgtagcg gcccatcagg gtctgggtgt
      901 ggctgatatt gtagtcaacg cgggcgtttt cctggcgcca gtcgattggc gaggtgaccg
      961 atgtggccca gttaggaata ccaccacttg ttccgttgat gttcggagcc ggaaagttca
     1021 gcagaatgtc ctggccggcc ggactgatcg aattgaattt atagggattg ccgggcgcct
     1081 gcagcgcgac cgggatgttg ggctgagcca cattgcattg gtcaaccttg tggcccggcg
     1141 cgccggtgaa ctcggggccg ctgaaatcac cattgatttc ggcagtcgtc ggaacgcacg
     1201 aattacgggt ctgaccgcgc ttttcgtaat tccattcctg gctgtaaaag acgaacagtt
     1261 tgtccttctt gatgggcccg cccagcgaga agccgaaatc gttgcgctgc accacattct
     1321 tgaactggtt ggaaggcgct gcaccgccgg cgactgccaa ttgttcgttg gcatgagcga
     1381 aatactcata cgcgttcaag gccgtgttgc ggccgaagta gagcacgtcg ccgtgccact
     1441 ggttcgatcc gctgcgggta acaatattga tgacagcgcc ggaagcctgt ccatattccg
     1501 gaccgtagga gttacgcagc atcttgaatt cgctgatcgc ttcgatcgac ggatagatca
     1561 agatggtgcg gttggaacca acgtcgttgt tgttggcgcc gtccaccagg aacaagttgt
     1621 tggtggttgc gttgccgttc accgaaaagt caacgccggc cagcaggccc ttgttgttgg
     1681 cgctgaagtt gttggccgcg gaaacgccgg gctccaactg ggtcaactga acaaagctgc
     1741 gtccattcaa cggcagttca cgaacctgct cgccagtgac aacttcaccc aacgccgcgt
     1801 tgtcagtctg aacctgcact tcgctggcgg ctaccgtaac ctgctccgag gtgctaccga
     1861 cctgaagctg aatatttaat acctgcgtgc tcgaagtatg taggtcgacg tttttcgaga
     1921 ccgactcctt gaagttcgga gccttgacgg tcacggtgta cgtgccgacg gcaatttcag
     1981 gagcaacata ctcgccctga gtgttagtgt ttacggtgcg tgtctgtcca gtttcggagt
     2041 tcgtaatatc taccggaaca ttggccagaa cagcgcccga cgggtcggta acaacgcccc
     2101 tgatcgtacc tgatgtcttc tgcgccatta gcggcacagc caacagcgat acgatacaaa
     2161 gggtcagtaa cagccaactg cccaaacgat tcatttgttc ccccaccaga aacgattttt
     2221 gttacaacgt gcgccgtcgc ggacctcacc gtgagttccg ccgcacccgt caataccaac
     2281 acgcgatcga gtaaattcgt tcaccctgac acccaagaag taccgattct ttaatacaag
     2341 cgtgcgattg agttcgtaca atcaatacgc tgcacgaaag taccgattct ctaattcaag
     2401 cgaatgggcc gagtaagttc gttaaaccag aagcccagca gtaccgattc tctacagtta
     2461 gagaaccgat tctgttcaca accccgtgac tagttaattt atgttattga cgggtatggc
     2521 tgtcaacaaa aaaacacaat cttttgtgga attcgagcat cccagcccca aaaacgcaca
     2581 taaattcccg aaatgggtcc gattcgtgcg atttttcctg aattggctca ccccttaaca
     2641 aaatcggttg cgtaaatcgc agagtcgtgg gtacattcaa gcttcgtgtg atgcgcgcat
     2701 gccgggacac tccaaaccac ccaataacgg cggaaccact ctcaaggagc tggctaaaca
     2761 cctgggactg acgaagggca cgatttctgc cgtcctgaac gactccccgt acgcgaaggc
     2821 gattccccag cacaccaagg accgcatcat cgcggctgcg cgggaactca actaccaccc
     2881 gaacttcttt gcccggtcgc tgcgtaagaa gcgcacgttt acggtgggcg tgatcgccgc
     2941 agaaattggc gatgcctacg gcagtgtggt gatcggcggc attgaatccg cgctcagtga
     3001 gcgcaagtat tttttcgtga cggtcgtaca ccatcatgac ccgcaactgt tgcaccagta
     3061 ctctgacatc ctgttggcgc ggggagtcga gggtttcatc accatcgaca ccaccgtgaa
     3121 ccagacccag cgggtaccca caatcgtaat ttcgggacat cgccagattg aaggcgttac
     3181 caacatcact ctcgatcact ttcaagccgc ccatttggtt ctggcacatc tcaccgaatt
     3241 gggccactcc gaaatcgcga tcatcaaggg ccaaccgctt agcgtggact caggcgcacg
     3301 gtggcatggc ataaccgagg cggcgcgcgg gttgggaatc cagttgcgga cagaactcac
     3361 cacccagttg gcggttgacg atccttcgcc ccaggaaggc tacaagacca ccaaagaatt
     3421 gttgtcgcgc aaggttccgt ttaccgcgtt gtttgcgtat aacgacatct cggcggtggg
     3481 agcgatccgg gccatcaagg agtccgggct gcgcgtgccg gaggacatat ccgtcgttgg
     3541 cttcgacgac gttcgcgaag cggcctacca tgtgcccagc ctgaccacgg ttcgacaacc
     3601 gctgcgaaag attggcgtca tggcggctga aacgctcatc gatcgcattg agggacggga
     3661 cggacacgag gctggaatcc tggtggaacc ggaactcgta gtgcgagagt ccagtactgc
     3721 ggtgagccca actccgctcc atcgcaatag cgagcgaact cgcctcagtt gaggcgaatt
     3781 ccgccacctc aataaatact tcatggaatg gccgagacta ttgcggcgtg accgtctggc
     3841 cctgcgaggc atcggtaggc cgcttgttcc gcagcttttc ataaagttga agctgttgct
     3901 gggcctcgtc tttcatgccg agcttgttat aaacccgggc tagttcatag tggttttgtg
     3961 gctccgcagg gactttctcc accgcctttt ccagcagggg gcgcgccttc tccgggtcgt
     4021 tctgctgcag atagagttga cccagcgcgc cgagagcccg ggggttgtcg ggattgacgg
     4081 cgagtgccca gttcatctgg tcgactgcgg cttcgatgtc tccctgctgt ttggcgatca
     4141 gccccaggta gtagcgggcc gtcgagtctt ccggtccctg tcccagggaa gccgtcagat
     4201 agtggctggc ctcgggcaag tttccggtgt tgtaatcggc cagtcccatt gccaattgcg
     4261 cccgcttgtc gttacgagca ttcgccagaa acttttcgag caaaggtttc gcttccccaa
     4321 aatggttggc ttcgatcatt accgtagcca gtgccatggt gctcgccgga tcattgggac
     4381 gaagcttgtg ccaacgctga gcgacttcgt gggccgccac taagtcatgc ttcttgattt
     4441 cgacgtagac cacttgttgc agcagatcac tgcgaggtgg gccaaccgcg agcgcccggt
     4501 ccagaaagcc agcggccgcg tcccagtcat tcgccatttc agccagcgcc gctgccgcgc
     4561 tcagcgcttc gacggacttg gggtccattt gcagcgcttt catcaccgtc cctctggcct
     4621 gctgttggtc tccctcggct gcttgcacct gagcgaccgc cgcgaggaag gcgggcgtgt
     4681 tcttttgatg gctctgaaca aacttgagca actcagcggc gtcattcgtc atgccatgct
     4741 ggaccaaggc ggtcgccagt tgcggcacga tttccggatc agcggaggca tgcgccagta
     4801 catcgcctac tgctttgtgg gctggctcag acttgccgag cgcgagatag tccactgcca
     4861 gcaccggcaa cagtttggtg gaccgtgccg cgggcgggat agcggacgct actcgcagcg
     4921 acttctcata ctcacccgcc tcctgataca gcatcgcaag ctcagacgcg ttgccggcct
     4981 tctgcggtgc gagtttctgg caggcttcat actgcgcaat ggcctcttcg gtgcgtccct
     5041 catcctttag caatgacgcc agacttacgc acgcctcttc cgacttcgga ttgtgttgag
     5101 ccgcctgccg gaataatgtc tcggcctcgt cataccttcc ctgcttcagc tgaacttgcc
     5161 ccagcaggtc ttccgcccgg taattggcgg aatgtttctg caagatgtcc tgcaactggg
     5221 tctcagctgg ttccagcttc gattgtttga tcagggtttc gatggcagaa aagtttgcct
     5281 gatcgacagt cgttgcagcc aggttcatcg cgaaacagaa ggttacagcc aatgccacca
     5341 agcctcgggc aaacccgttt ccaggccgag acaatgaaaa ccgagcagct ttttcaggcg
     5401 aaatcactct gcggaactca aggggaacaa tcacgagagt aagcgggcgc cagaagccgc
     5461 gtcaaggcgg ccccgctgtc tggacgaccc tggccacgat agaatgacca cagcctctac
     5521 tcgggtttct cctctatgcg tgtgtcggta gtgttggctt tttctctgct gatgagcagc
     5581 gctgccgtcg cacagaccgg tcccattcat tttcacaacg ctaataaaga agcaggtctc
     5641 ccgatgccgg gctcgaccca gcccgagatg aagtacatcg tgcaacagat ggccggcggg
     5701 gtcgcgctgt ttgactgcga caacgatggc aaactcgaca tcgcggtggt cacgccttcc
     5761 actattcccg gctatcgcgc cggaggcgat ctcatggtcg cgctctacca ccaggatccc
     5821 ggcttcaagt tcaccgacat cacgaagtcg gcgggactga cgcgcaaggg gtggggcatg
     5881 ggactggcag tggccgacta cgacaatgac ggcttgcccg acctctatgt caccggctac
     5941 ggcggcaatg ccctgtatca caatctcggc aactgcaaat tcgaggacgt caccgaccgc
     6001 gcaggcgtcg ccggcggggg cttcagcacg ggcgctgcct ggggcgacta cgaccgcgat
     6061 ggaaatgttg atctcttcgt ctcgcgctac atccacaccg acgtcgccac gctgggcgaa
     6121 atcaagacga gctacaaagg attggtcgtc gaggcgccat gggggatgtc gggcgaatcc
     6181 gatttcctct tccacaaccg gggcaacggt acgttcgagg aagtttccag gaaggccggg
     6241 gtcagcgatc ctgaagggcg actgggcctg ggcgtggtct ggggagacta cgataacgat
     6301 ggctggcccg acatcttcgt ggccaacgac accgaaccca gctacctcta ccgcaacaag
     6361 catgacggca ccttcgaaga tcaggcactg ctttcggggg ccgcggtcag tgccgaaggc
     6421 aaagccatgg gctccatggg agtagacttt ggcgacttcg atcgcgatgg ccgactcgat
     6481 attacggtcg cgacatttgc ttatcagcct gacaatcttt ttcacaatgc cggggacgaa
     6541 ttcaatgaca tcacctgggc cgccaagctc ggacagccga cgttccgctg ggtaaagtgg
     6601 ggcaccggct ttgtggattt cgataacgac gggttgcagg acatactggt cgcctccggc
     6661 cacatctata cggcgatcga ccagttgccg ggcgagcctg cattccgcga gccgatgatc
     6721 ctgtttcgca acacagggca actgaagttc gatgacgtca gcgacacgtc cggagtcaat
     6781 gacgagccct tacaatcgcg ccgggggatt gcgttcggcg acctcaacaa tgacggcaac
     6841 attgacgccg tggtgttcaa cgtcaacggg ccgccctcga ttttgatcaa cgacactagg
     6901 aactcgaacc atcgcgtgat gttcaggctg gtgggaacga cgagcaatcg cgccgccatc
     6961 ggagcccgcg tgacgatcac cgcaggcggc gttacccagt tgcgcgaggt caagggcgga
     7021 aatagctaca tctcgcagag cgatctgcgc c
//
LOCUS       DASRKK010000183         7740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_403547, whole genome shotgun sequence.
ACCESSION   DASRKK010000183 DASRKK010000000
VERSION     DASRKK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403547"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..858
                     /locus_tag="VFC15_03990"
     CDS             532..858
                     /locus_tag="VFC15_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM09352.1"
                     /translation="MERWHKSLKQECIRPLTPLTLDDARRLIQAYVDRYNTVRLHSVI
                     GYVTPQDMLLGRHAEIHAERDRKLEQARRLRQLRRQQVATFLDSPITMTSPEETEAGS
                     AGRQPC"
     gene            1320..2432
                     /locus_tag="VFC15_03995"
     CDS             1320..2432
                     /locus_tag="VFC15_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09353.1"
                     /translation="MASSPRPGNVPLQRENPNSNTPPPTDHGEIGPIWYSFDLAHKRV
                     QPGGWTHQVTQRELPSSTELTGVNMRLTAGSFRELHWHTADEWSCMLYGNARVTVLNP
                     DGTIFIDDVRQGDLWYFPAGFPHSIQGLGPDGCEFLLVFDQGMFSEDNTFLISDWIAH
                     TPPSILSKNFGLDESSLAKLPTGERYIFPASLPRSLAQDRIAVGGPQVQSPIQYTFKM
                     QSMPPTKKTAGGEVRVVDSHNFPASRNIAAGLVNVKPGGMREMHWHPNASEWQFYIAG
                     SARMTVFVPVGSARTMDFHANDVGYIPAVAGHYVENTGDTDLVFLEMFRASEFLDFSL
                     NNWIRRLPPEMVTSHLNLDENEIKKVPAANQSLIGR"
     gene            2486..3469
                     /locus_tag="VFC15_04000"
     CDS             2486..3469
                     /locus_tag="VFC15_04000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09354.1"
                     /translation="MQVHSDIPLRQFGNKDVRISALGLGGHHLGDAADEVTAMRIVQE
                     AVDGGITFFDNCWEYNLGKSENWLGKGLKGRRDKVFLMTKVCTHGRDASLAMQMLEQS
                     LNRLQTDHLDLWQIHGVAFDNDPDLFIRPDGGAEALMKAKEQGKVRFVGFSGHKHPDI
                     HLKMLNTGFPFDSVQMPLNVFDSQFQSFENHVLPELARRGIAALGMKPISGHGEPVSR
                     GVITAQEALRYAMSLPVATTITGIDSLEVLHQNLAIAQGFAPMSQQEMQALRDRVKPE
                     AGDGRFELYKVSLKFDNPESRLAHDFPIDMRQAETKEMMQAAKNTGHPYPR"
     gene            3530..4489
                     /locus_tag="VFC15_04005"
     CDS             3530..4489
                     /locus_tag="VFC15_04005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09355.1"
                     /translation="MEEEKSNQRGNTVGLVPRRALGRTGMQVSALALGGYHLGSAKNQ
                     QEVNDIVARAMEAGVNFFDNAWDYHSGHSESVLGTALKGKRQQVIVMSKVCTHGRGKD
                     LALQQLEESLRRLQTDYLDLWQIHEVIYENDPDLIFRSDGVAEALVKAKQQGKVRAVG
                     FTGHKDPKIHLRMLNFDFPFDTVQMPLNCLDATFRSFEVNVLPVAEQRGLGVLGMKSM
                     GGSGEIVTHGAATPEQALRYAMSLPVSSTISGVDSIQVLDQNLEVAREFRAMPPGEME
                     ALRKRCSRFASDGRYELFKVTKKYDGDVGREQHGFPLAEELPA"
     gene            4744..4947
                     /locus_tag="VFC15_04010"
     CDS             4744..4947
                     /locus_tag="VFC15_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09356.1"
                     /translation="MQKNSGARKHKEALSATVRAIATAMEMRDPYTAGHQSRVAEIAF
                     ANGRELGWLQALRMAALIHDIGR"
     gene            5058..5198
                     /locus_tag="VFC15_04015"
     CDS             5058..5198
                     /locus_tag="VFC15_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09357.1"
                     /translation="MMGQRTRSESLFYYLRIEDQVPKNHLLRLIGRHVSFDFVRENLH
                     DS"
     gene            complement(5256..6185)
                     /gene="mdcH"
                     /locus_tag="VFC15_04020"
                     /pseudo
     CDS             complement(5256..6185)
                     /gene="mdcH"
                     /locus_tag="VFC15_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
     gene            complement(6182..6919)
                     /locus_tag="VFC15_04025"
     CDS             complement(6182..6919)
                     /locus_tag="VFC15_04025"
                     /inference="COORDINATES: protein motif:HMM:NF002332.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HZM09358.1"
                     /translation="MDRFPEPSSLGLAMFSDQVCSVRLHDLLEVDPERFIATEGRGKS
                     IPGWVEERLLAFPFVVVRRGMATERKIPVGVRGETRNQRWAASCHRACVKKIITPPQL
                     LQCAVSESRAQAIPALRSLFLLKVRWRDFDLPWGPGGSIAFELATGRQVANPKSDLDI
                     VVYADNRLTKNEAKRLCAVTADLPSEIDIRVETPRCGFSLREYSSANSNILLRTPTGG
                     MLGRDPWQHDLRIANANQVVKSERGQR"
     gene            complement(6889..>7740)
                     /locus_tag="VFC15_04030"
     CDS             complement(6889..>7740)
                     /locus_tag="VFC15_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZM09359.1"
                     /translation="DNRNVKSLVTFVMSFAIPCYLYVTVAATPRRLLWNQTKVALVLP
                     IVYAGLFVATYVFARDTASNRAVLALTLGFPNAAAIGPPLLQSLYGERAGVTVAVAIA
                     IGSVTVSPVTLAILESETGAGKKISVLARIRLSLWRALKRPIVWAPLLALVAVATNFS
                     LPGYFERSLTIFGDATAGTALFMTGLVVSAQHFSFNWSVGWAVCGKNLLQPALCLLMA
                     DLVRMPREETRYVVLISAIPCGFFGIFFGEDFDATPEMASSSLVASYLLGIFTLAGWI
                     VFLSHLH"
BASE COUNT         1784 a         2135 c         2166 g         1655 t
ORIGIN      
        1 ggctacgctc gttacccaag gggactccat cgaggtcgca atcaagaggg ccgaccagtt
       61 gatgtatacc agcaaatcga gcgggaggaa taaagtcact gtcgggtgag cagtgactcc
      121 aaggcgctgt ccttttttgc cagaacagaa agtcgttact tgctgaccag agcgtcatgg
      181 tcgtcgagat actgcatttc atatcctcag ttgtcacgtc cctctgcgac atcccatcac
      241 tgacaaacca tcctgtggct aatcaaaact aaacgccgct aaacaacaca tccagcttta
      301 agttccctta cccgcggcaa cgctaaggta cgctttcatc gcgagcctac tgctgttcgc
      361 ctcagtgatc ggttttgtcc gtgtgtttct tcttgcgaga atggcggaca tgacgacgct
      421 tttctttcgt tctccaatta acaccatgtt ctatctgact gagttcattc gcatctcggg
      481 gatgacgcac gtgcgtacct cgccttatta tccgcagggt cggatggcaa gatcgaacgt
      541 tggcacaagt cgctcaagca ggaatgtatc cggccactca cgccgctgac tctggacgat
      601 gcgcggcgct tgattcaagc ctatgtggat cgttacaaca cggtgcgtct gcatagcgtg
      661 atcggctatg tgaccccgca ggacatgctg ctggggcgcc acgccgagat ccatgctgaa
      721 cgcgatcgca aactggaaca ggctcgccgc ttgcggcaac tccgccggca acaggttgca
      781 acctttctcg attcaccgat tacaatgact tcgccagagg aaacagaggc gggctctgct
      841 gggaggcaac catgctgagg gatactctgg tggggctcat cgagccgatg ttacgcggcg
      901 cgggagttca ctgctctcgc gctggttttt ccccgtgctg cagcagacat cggacgatag
      961 acccccaatg cctggaaaat ccccgccccg caggggtgga atctacacta acggagaacg
     1021 cccgttctcc atttcacgct gagccaagac agtggcagtc tcgctatgac gaaggcgtat
     1081 atttgtttgg ttccctaaat tgttacgtgg ccctgtcatg gaaattgaga cgtgagcttg
     1141 cgttttcggc gcggtctcgg cggcaggcac aacattaagt tgcaggcctg accaaaactg
     1201 gaatagtgga ggagagtatg accaaccgaa ctgttgaagg cagattgtcc gggggaagat
     1261 cttcaggtgt gggacatgcc gcagttgcga aggcagcgcc tgcggcatcg agtagtggca
     1321 tggcgagcag tccaaggcca ggaaacgtgc ccctgcagcg agagaatcct aactcaaata
     1381 cgcctcctcc caccgaccat ggagaaatag gcccgatctg gtactcgttt gatctcgcgc
     1441 ataagagagt gcagccgggt ggatggacac atcaggtaac gcagcgtgaa ttgccgtctt
     1501 cgaccgagct tactggcgtc aatatgcggc ttaccgctgg cagctttcgt gaactgcatt
     1561 ggcatactgc cgatgagtgg tcctgcatgc tttacgggaa tgcccgggtc acggtcttga
     1621 atcctgatgg gaccattttt atcgatgatg tgcgccaggg cgatctctgg tatttccctg
     1681 caggatttcc acactctatc caggggctcg ggccggatgg ctgcgagttc ttgctggtct
     1741 tcgatcaagg catgttttcg gaagacaata cctttctaat ttcggattgg attgcgcata
     1801 ctccgccttc catattgtcg aagaattttg ggttggatga aagctccctc gccaagctcc
     1861 ccactggcga acgttacatc tttcctgcat ctcttcctag atccttggcg caggatcgaa
     1921 tcgcagttgg cgggccgcaa gtacagtcgc ctattcagta caccttcaaa atgcaatcta
     1981 tgcctcccac caagaaaaca gccggcggcg aagttcgggt cgtcgattcc cataacttcc
     2041 ctgcgtcgcg gaacatcgct gccggtctgg taaacgtcaa gccaggagga atgcgggaga
     2101 tgcactggca tccgaacgct tctgagtggc agttctacat cgcaggaagt gcccgcatga
     2161 ccgtgttcgt tccagtagga agcgcgcgta ctatggattt tcatgccaat gatgtgggct
     2221 atattcctgc ggtggcggga cattacgtcg aaaatacagg cgataccgac ctggtgtttc
     2281 tggaaatgtt tcgagcgagt gagttcttgg acttctctct caataattgg atcaggcgac
     2341 taccgccgga aatggtcacg tcccatttga atctggatga aaatgaaatc aagaaagttc
     2401 cagctgcaaa ccaatcactg atcgggagat agctgcaatt cgcaacaatc gactgtcgaa
     2461 gtcatcaatc tgggagtgat ctgcgatgca agttcattcc gatattcctc tgcgccaatt
     2521 cggcaacaaa gacgtccgga tctccgcgct aggtttggga ggtcatcatc tcggcgatgc
     2581 agccgatgag gtgacggcaa tgcgcattgt gcaagaggcc gtcgacggtg gcataacgtt
     2641 ctttgacaat tgctgggagt acaacctcgg caagtcggaa aactggctgg gcaagggctt
     2701 gaaggggcgc cgggacaagg tgttcctaat gaccaaggtc tgtacccatg gccgggatgc
     2761 aagtcttgcc atgcagatgc tggagcagtc gctgaatcga ttgcagacgg accatctcga
     2821 cctgtggcaa atacacggag tggcattcga caacgatccg gacctgttta tccgcccaga
     2881 tggcggagct gaggccctga tgaaggccaa ggaacagggt aaggtgcgct ttgtcgggtt
     2941 ctccgggcat aagcacccgg acattcacct aaagatgttg aacacggggt ttccattcga
     3001 ctcggtgcag atgccgctaa atgtcttcga ctctcaattt cagagcttcg agaaccacgt
     3061 gctaccggaa ctggcacggc gcggcattgc tgcgctggga atgaagccga tcagcgggca
     3121 tggcgagccc gtgagccgcg gggtgattac cgcacaagaa gcgttgcgct acgccatgag
     3181 cctgccggta gctactacca tcacaggcat cgacagtctc gaagtcctgc accagaacct
     3241 ggccattgca caaggttttg cacccatgtc gcagcaggag atgcaggcgc tgcgggatcg
     3301 ggtgaagccc gaggccggag atgggcgctt cgagctttat aaggtgtctc tgaagttcga
     3361 caacccggaa tcgcggctgg ctcacgattt tcccatcgat atgcggcagg cggaaacaaa
     3421 agagatgatg caggcggcca agaacaccgg ccatccgtat ccccgatagg gtgatgagcg
     3481 cagagcagaa gtaagaggaa gctcctcacg ggaacttgga gagtaggaag tggaagaaga
     3541 gaagagcaac cagcggggga acaccgtggg tctcgtgccg cgtcgggcgc tcggtcgcac
     3601 aggtatgcaa gtgtctgcct tggcgttggg tggctaccac ctgggttcgg cgaaaaacca
     3661 gcaggaagta aatgacattg tcgccagagc aatggaggcg ggcgtcaact tctttgacaa
     3721 cgcctgggac taccacagtg gccacagcga aagcgtcttg ggaaccgcgc tcaagggcaa
     3781 gcgtcagcag gtcattgtca tgagcaaggt ctgcacccac gggcgcggca aggacttggc
     3841 cttgcaacag ttggaggagt cgctgcggcg cttgcaaacc gactatctcg atctgtggca
     3901 gattcacgaa gtcatctacg agaatgatcc tgatcttatc ttccggtccg acggcgttgc
     3961 cgaggccttg gtcaaagcca agcagcaggg caaagtgaga gctgttggtt ttaccggcca
     4021 taaggatccg aagattcatc tcagaatgct gaattttgac tttcctttcg acaccgtgca
     4081 aatgcccttg aattgtctgg acgcgacgtt ccgcagcttt gaagtcaacg tgcttccggt
     4141 ggccgagcag cgcggtctcg gcgtgcttgg aatgaagagc atgggcggca gcggcgagat
     4201 cgtaacccat ggagcagcta cccccgagca agccttgcgt tacgctatga gtctcccggt
     4261 atcatcgacc atcagcggcg tggactcgat tcaagtgctg gatcagaatc tcgaggtggc
     4321 ccgtgaattc cgcgccatgc ctccaggaga gatggaggcg ctgcgcaaac ggtgcagccg
     4381 gttcgcttcg gacggtcgtt atgaactgtt taaggtgacc aagaaatacg acggcgatgt
     4441 cggccgcgag cagcacggtt tccccttagc agaagagttg ccggcgtaag cagtgaagtc
     4501 aaatcgcaac tgaaaattac ggagacagtc aaaagcttcc acccgtggag gaacgagcgc
     4561 ggcgctgggg catacgttct gccacggtat ttcctttgcg cgccgaggat gggcgtcgag
     4621 ttgtacttgt gtctacgctt caccgcccta cacctttgag tctgtcgcgg tcgagggact
     4681 cacccacctc gcctcggaga ttggcgtcgg tcttcaaaca cttccgccgg caagcaaacc
     4741 tccatgcaga agaattccgg cgcgagaaaa cacaaagaag ctttatcggc aactgtaagg
     4801 gccattgcga cagcaatgga aatgcgcgac ccttatacag cgggacacca aagccgggtt
     4861 gcagaaatcg ccttcgccaa cggcagggag ttgggctggc tgcaggcatt gcgaatggcg
     4921 gcactgattc atgatatcgg gaggtaggtg ttgaaaaagt cgctgagttg gtgcttgtga
     4981 gtgcagtgca ggaacgtcat aagcagacga gagtttctga tgctgtgctt gatcgccatt
     5041 ctcggaggct catgcccatg atggggcaac gcacccgctc ggagtcgctg ttctactact
     5101 tgcgcatcga agatcaggtt cctaaaaacc atttgttgcg cctcatcggc cgccacgtca
     5161 gtttcgattt tgtgcgtgag aacttgcacg actcctaggt cttccttctt tcgagcgacc
     5221 atttcagact tccctctggc aacctctgcg ctgagtcaaa taactccgac ttgatcgacg
     5281 atccgcagta catacttcgt tgaggtttca ccgaccgcga cggctctcac atctggaaag
     5341 gcttcatgag ccagttcagt caaaacgtgc cccggcggca attcgaggaa cagccgacag
     5401 cctaattctt cgagcacggt agtcgcatcg tgccaacgta cgctgtgcgc gatgttgctg
     5461 gccaagtcgg ccgctatgcc ctctgcagat cgcaatggtc tagcgttcac gttgctcacg
     5521 tagattaggt taggaggttg caacgcggtt gtccgtaaac tctctgtcaa ggcttcagcc
     5581 actgactcca gcagcgggca gtgcgaaggc acggacacct gaaggcgctc cgccttccgc
     5641 gcgccactgt tctgggccgc acgcagcacc tgattgatac cttcgttcga accggctatg
     5701 acaatctgac atggggcatt gacgttggct atgtatatcg gcatttgccg ggcgtggact
     5761 tcgtcaacca aggcggtcac ctatctttcg gtcaatccaa caatggctgc tagtccgtac
     5821 cctttcggat agagcttcat catcatttct gcccgctgtc gcacaaggtg gaccccatcg
     5881 ctcaggcaga tcgtcccact ctccaccgca gcggcaaagg ctcccacgga cagtccggcg
     5941 actgccacgg gacggacgcc ctcttccacc aggacgcggg acatcgccac agcacaagtg
     6001 aatagtgcca actggacgga gagggttgac tgcaatgccg tgtcggagtc aaggtcgaac
     6061 acattctgac tcaaacattg gctcacttcg tcgcaggtcc gcctaatggc tggatggtcc
     6121 ggtagcctat gcagcatccc aggaacctgc gagccctgcc ctggaaacag aaaagctacg
     6181 ctcatcgctg gcctcgttcg cttttcacca cctgattcgc atttgcgatt cgaaggtcgt
     6241 gctgccacgg gtcgcgcccg agcattccgc cggtgggcgt gcgcaaaaga atgtttgaat
     6301 tcgcgctcga atactctcgc agagagaatc cgcatcgtgg tgtttccacc cgaatatcga
     6361 tctctgaggg aagatccgct gttacagcac aaagacgctt tgcttcgttt ttcgtcaggc
     6421 gattgtcggc atagaccacg atatcgagat cgctcttggg gttggcaacc tgcctgccgg
     6481 tggcgagttc gaaagcaatg ctgccaccag gcccccacgg gagatcgaaa tctctccagc
     6541 gaactttcag aagaaacagc gaacgtagag caggtatggc ctgcgctcgc gactctgaaa
     6601 ctgcgcactg cagcagttgc ggcggagtta tgatcttctt cacacaagct cgatgacagg
     6661 aggcggccca gcgctgattt ctcgtttcgc ctcgcacacc cacgggaatc tttctctccg
     6721 tcgccatacc gcgacgaaca accacaaatg gaaacgctaa taatcgctct tctacccacc
     6781 ctggaatcga cttgcctcgt ccttcagttg ctatgaaccg ttccgggtcc acctccagca
     6841 gatcatggag tcgaacggag caaacttgat cactgaacat ggctagccct aatgaagatg
     6901 gctcaggaaa acgatccaac cggccagagt aaagataccg agcaagtagc tcgctacgag
     6961 gcttgaactt gccatctccg gggtggcatc gaagtcttct ccgaaaaaga ttccaaagaa
     7021 gccacatggg attgcactga taaggaccac ataacgagtc tcttcccgtg gcattcgcac
     7081 caaatccgcc atcaggaggc aaagtgccgg ctgaagcagg ttcttgccgc agaccgccca
     7141 ccccaccgac cagttgaagc tgaagtgctg cgcggatacg accaaccccg tcataaacag
     7201 ggccgttccg gcggttgcgt cgccgaaaat ggttagactg cgctcgaaat agccaggcag
     7261 actgaagttg gtcgcgactg caaccagggc caacagtggt gcccacacga taggcctttt
     7321 caatgccctc cataacgaca ggcgaattcg agcaaggacg gaaatcttct tccctgctcc
     7381 agtctcactc tcgagaatcg ctagagtcac cggcgagacg gtgacggagc caatcgcgat
     7441 agctactgcc acagtgaccc cagcccgttc gccataaagg gactgcagca gcggaggacc
     7501 aatcgccgca gcattgggga accccaacgt aagggccaga acggcgcggt tggacgcagt
     7561 atcgcgcgca aaaacatacg tcgccacaaa aagacccgca taaacgattg ggagtaccaa
     7621 ggctacctta gtctgattcc ataaaaggcg gcgcggagtt gctgccacgg tcacatacaa
     7681 gtaacaagga atcgcaaagc tcattacgaa ggtgaccaga cttttcacat tcctgttatc
//
LOCUS       DASRKK010000184         7380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_403640, whole genome shotgun sequence.
ACCESSION   DASRKK010000184 DASRKK010000000
VERSION     DASRKK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403640"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFC15_04035"
     CDS             <1..187
                     /locus_tag="VFC15_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09360.1"
                     /translation="IERVQWRGTLAGCNVKVHQHLDGTISLTHEPHRLGRYSAQAAAM
                     TSTRNAAAKNAVEKTAG"
     gene            complement(358..1392)
                     /gene="tdh"
                     /locus_tag="VFC15_04040"
     CDS             complement(358..1392)
                     /gene="tdh"
                     /locus_tag="VFC15_04040"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZM09361.1"
                     /translation="MKALVKSRAERGLWLDDIAEPTMGINDVLIRVRYAGICGTDVHI
                     YQWDEWAQKTIPVPMAIGHEFVGEIVEVGSNVNDFYPGDIVSGEGHVTCGRCRNCLAG
                     RRHLCAYTSGVGVNRPGAFAEFIALPMTNIWRHDPGINQEVAAIFDPFGNAVHTALSF
                     PVLGEDVLITGAGPIGIMAIPVVRHAGARHIVITDVNPFRLELARKMGATLAVNTKDS
                     PLGEVQKQLGMREGFDVGLEMSGNPAALQEMIANMSHGGKIAMLGIPAKPMTMDWRAV
                     IFNMLTIKGIYGREMYETWYKMTVMIESAVDISPVITHRFSYREFQKGFDAMISGQAG
                     KVILDWTHVG"
     gene            1552..2289
                     /locus_tag="VFC15_04045"
     CDS             1552..2289
                     /locus_tag="VFC15_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZM09362.1"
                     /translation="MLIEIGHVDAIFRYPVKSMAGEALEVANLDWHGLEGDRRLAFRR
                     MNDHSDFPWLTASKLPDLLLFAPHRDTGAAQADLPTHIRTPDGEAMPVFSENLAAEIG
                     RRYGAPVQMMQMKHGIFDEASISVIASDTVSEIARLAVRSLDVRRFRPNVVVRLLRSG
                     PFQEDEWLGGVLSFGEGDDAPAITVTLRDERCSMVNLDPDSASPAPEVLKAVVRAHQN
                     TAGIYGAVTRIGRLAVGQAIFVHAAGI"
     gene            complement(2438..4543)
                     /locus_tag="VFC15_04050"
     CDS             complement(2438..4543)
                     /locus_tag="VFC15_04050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZM09363.1"
                     /translation="MSIRHDCWRTGALIGVAILLMLTVAGSAFAANGQVLANSTPRFV
                     QSAQNLGPESPSKVINITVRLQEHNIAERDALLKQLYDPKSPLYQHWLTPAQYAARFG
                     PAAQEATVVKDFLASHNLVVTSAHQNHFYVNAQGSVADVQKAFNVQINRFNVNGKTIY
                     SNTSNASVAGPAGAFVSSVQGLHPVAMKPRSVRPVDPETGQPFPEMPLGATAQKGPLT
                     GPAPSLFYENQCYRGVETHAFTTSGHLPAAYYSGNRYGGDIHGGAGHYPPCGYEPAAM
                     QTAYGLNPLISAGNDGTGQTVVIVDAWGSPTATADFGVFSATFGLPTVGFTDHNPFGA
                     DTTNTNWATETTLDIEWSHSMAPGAGVALVRSIDNYDNNLQNAIQYALDNQLGNQISN
                     SYGSDEYENDAAGMQSWDDLNAEGAALGVSINFSTGDNGDFYRAVGAYTVSVPSDSPH
                     ATAVGGTSDFLNPDYSMKFQTGWGTDLTRIAAPNGTNPPTIPLVCASTLQPGYCFYFG
                     AGGGQSTYFAKPSWQSSLTGAGRQQPDISMTADPYTGLTIVYSYNNPGTYSVSVIGGT
                     SASCPMFSGVWAIVNQKSQQVHGKPAGLAAPYLYSMPGGSIKDVKQSSPYTGTNLTGI
                     IMQAGKAPLYESAVAISTPDINTQFTSAFYQGTSTRWYAISFGTDSSLLVTNGWDNVT
                     GVGTPYGAAFVNGVVNQVH"
     gene            complement(4670..5284)
                     /locus_tag="VFC15_04055"
     CDS             complement(4670..5284)
                     /locus_tag="VFC15_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM09364.1"
                     /translation="MRRITYLSAMTVLALCILAPGAFANSGFGVPFPSNTTFYCSQTN
                     PPCDFMGNNGGLTKPMWTAGDFITETFFSTVPWVNNLEANWGVVDDFGGIPGATYEND
                     VYINGNFVGYFLLQDCNYCSTLYTVTGTAFFAPIYGAGSYNLSIVLAQTAPGGAGSEW
                     FSVLTTNGGASTAFFSTTPEPSSILLLGSGLIGLAGVVRRKLTM"
     gene            complement(5590..5862)
                     /locus_tag="VFC15_04060"
     CDS             complement(5590..5862)
                     /locus_tag="VFC15_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HZM09365.1"
                     /translation="MTKKIVHRNRDKQLSRVRKIRGQIEAVERALSNEEEECADTLQL
                     LAACRGAINGLMAEVMEEHIREHLGRGKASEEVTDDLLAIVRAYLK"
     gene            6046..>7380
                     /locus_tag="VFC15_04065"
     CDS             6046..>7380
                     /locus_tag="VFC15_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZM09366.1"
                     /translation="MRQASPIRSDSWAAAVGLARSTLLRTIGALLCLAALSIATRAQD
                     PPADAGTPDAPTPRDTFFEHSNTAKWWVSGQANFVFQAHGDFYAAYSGTNSLHDTAEN
                     ATSRVLTLFTGYEFTPSSILYLDVEEAGWGGISGALGLAGFTNLDVVRNPQLGTQPYI
                     ARLMFQQIIPFSKEKIEIPRGPLTLATQLPARRLEIRFGKFSMADFFDNNVGGSDSHY
                     QFLNWTAANNGAWDYAADTRGYTVGAIFDYEDRNWGLRFAEALMPKVANGPNLDADVA
                     RARAENVELELRYKLLKKKDTSVRFLSYVNHANMGTYRDAIAIYLADPTQPPDIIATR
                     KQGTIKYGFGINFQQEFSSDVWAFGRWGWNEGQHESFAYTEADSTLQLGVYAKGTRWH
                     RSLDRLGATFISNGIGAYHQTYLADGGLGFILGDGGLNYGRETIEEAFYTIHA"
BASE COUNT         1601 a         2180 c         2057 g         1542 t
ORIGIN      
        1 gattgaacgg gtgcagtgga gagggacgct ggcaggctgc aacgtcaagg ttcaccagca
       61 tctggacgga accatcagcc tcacccacga gccgcatcgg ctgggacgct atagcgccca
      121 agccgcggcc atgacgtcaa cgcgaaatgc ggcggccaaa aacgctgtgg aaaagacggc
      181 tggatgaact tcaacaaccg gacatttcac ttgctatgaa aagcggacat tttaacttgc
      241 tatgaacatt cgcgcagaaa cctccacttc cgcgaattcc cgcatgccga cttgcccgga
      301 gcaatcccta aatgaaccac tcaaacgaca aaatcgaaaa cggttttggg caggctccta
      361 gccaacgtga gtccagtcca gaatgacctt tcccgcctgg cccgaaatca tcgcgtcgaa
      421 tcccttttga aactcccgat agctgaatcg gtgggtaatg accggtgaaa tatcgactgc
      481 cgactcgatc atgacggtca tcttgtacca agtctcatac atctcccgtc cgtagatacc
      541 tttgattgtc agcatattga agatcaccgc tcgccaatcc atcgtcatcg gtttagcggg
      601 aattccgagc atggcgatct tgccgccgtg actcatgttc gcaatcatct cttgcagggc
      661 cgcagggttc ccagacatct ccagtcccac atcaaatcct tcccgcatac cgagttgctt
      721 ctgtacctcc ccgagaggcg aatctttggt gttcaccgcc agggtcgccc ccattttgcg
      781 cgccagttcc aagcggaaag gattcacatc cgtgatcacg atatgacgtg ctccagcatg
      841 ccgtactact ggaattgcca tgatcccgat tggcccagcg ccggtaatca atacatcttc
      901 gccgagcact ggaaacgaca gagcggtatg caccgcgttg ccaaaggggt cgaagatagc
      961 cgcgacttcc tgattgatgc cgggatcatg ccgccagata ttggtcatcg ggagcgcgat
     1021 gaattcagcg aacgccccag gtcgatttac accaacgcct gatgtgtacg cgcacagatg
     1081 acgtctccct gccagacaat tgcggcaccg tccacaggtg acatgcccct caccgctcac
     1141 gatgtcgccg ggatagaaat cgtttacgtt cgatccaacc tcgacaatct ctccgacaaa
     1201 ttcatgcccg atggccatcg gcacaggaat cgtcttctgt gcccactcgt cccattgata
     1261 gatgtgaacg tcggttccgc agataccggc atagcggacg cggatcagca catcattgat
     1321 ccccatcgtg ggttctgcga tatcgtccaa ccacaatccg cgttccgcgc ggctctttac
     1381 caaagctttc atgaatgcct caaccgtgaa tgttttgtcg tgctctgcaa tgatacgaga
     1441 gcgatggtac cagtgaagtc tgttccgttg aaacacccag ctccctagga cgttcgattc
     1501 caaatcggga tcaaatattc ccacgcgtta caatcttcct acgcagcgag catgctcata
     1561 gaaatcggac acgtggatgc catcttccgg tatcccgtaa agtcaatggc cggcgaagca
     1621 ctcgaggtcg ccaacctcga ttggcacggc ctcgagggtg accggcgcct ggcattccgg
     1681 cggatgaatg accacagcga cttcccgtgg ctaaccgcca gcaaacttcc cgacctgctc
     1741 ttgttcgctc cgcaccgcga tacaggcgct gctcaggcag accttcccac gcatattcgc
     1801 acgccggatg gcgaagcgat gcctgtcttc agcgagaact tggccgcgga gatcggacgt
     1861 cgctatgggg ctcccgtgca gatgatgcag atgaagcatg gaatcttcga tgaagcgagc
     1921 atctccgtga tcgcctccga tacggtgagc gagatcgcgc gactcgcagt tcggagcctg
     1981 gacgtgcgac gatttcgccc gaatgttgtg gttcgcttac tgcgatcggg tcccttccag
     2041 gaggacgagt ggctgggcgg tgtgctctcg ttcggcgagg gggacgacgc tcccgccatc
     2101 accgtcaccc tgcgcgacga acgctgctcg atggtgaacc ttgatcccga ctcagcaagc
     2161 cccgcgccag aagtgctgaa ggcggtcgtt cgtgcccacc agaacactgc gggaatctac
     2221 ggcgcggtca ctcgcatcgg tcgactggcg gtcggacagg ccatattcgt ccatgcggct
     2281 ggaatctgaa gcgaatagcg attacggtcg ataaattgcc gggtgcccca gccagcgttc
     2341 cccatccgaa gttccccact agccgcattg ttcagcgcaa tcaaaatggg ccgccgaagc
     2401 ggcccactct gcagattgag aaacgactcc actcaactta gtggacttga ttcacgactc
     2461 cgttgacgaa agccgctccg tacggtgtcc ctacgcccgt gacattgtcc catccgttgg
     2521 tcacaagcag cgatgagtcc gtaccgaagg aaatggcgta ccaccgggtc gaggtgccct
     2581 ggtagaacgc gctggtgaac tgggtgttga tatcgggagt ggagatagcg actgccgact
     2641 cgtacagagg agccttgccg gcctgcatga taatgccggt gaggttcgtg ccagtatagg
     2701 gcgaggactg tttcacgtcc ttgatggatc cgccgggcat gctgtacagg tatggcgccg
     2761 ccaaacctgc cggcttgcca tgcacctgct ggctcttctg attgacgatc gcccacacac
     2821 ccgagaacat cgggcaggaa gcgctcgtgc cgccaatcac cgagaccgag taagttcccg
     2881 gattgttgta cgaatagacg attgtcagac cggtgtatgg atcagccgtc atcgaaatgt
     2941 ccggttgctg ccgtcccgcg cctgtcaggc tgctttgcca cgatggcttc gcgaagtacg
     3001 tactctgccc gcctcctgcg ccgaagtaga agcaatagcc cggctgaagg gtagatgcgc
     3061 agaccagcgg gattgtaggt ggattggttc cattcggagc tgcgatacgg gtgagatcgg
     3121 tgccccagcc ggtctggaac ttcatgctgt agtccggatt caggaagtca ctggttccgc
     3181 ccaccgcggt tgcgtgcgga gaatcggagg gaacgctgac ggtgtacgct ccaacggcac
     3241 ggtagaaatc gccgttgtct ccggtcgaga agttgatgga gacacccagc gcagcgcctt
     3301 cggcattcag atcgtcccat gactgcatgc cggcggcatc gttctcgtat tcgtccgagc
     3361 cgtagctgtt ggaaatctga ttgcccagtt gattgtccaa tgcgtactgg atcgcattct
     3421 gcaggttatt gtcgtagtta tcgatggacc gcaccagagc gacgccggct cctggcgcca
     3481 tggagtggga ccattcgata tccagagtcg tttcagtggc ccagttggtg ttggtggtgt
     3541 cggccccaaa gggattgtga tcagtgaaac ccacagtagg cagcccgaac gtcgccgaga
     3601 atacgccgaa gtcggcggtc gccgtgggag atccccaagc atcaacgatc acaaccgtct
     3661 gacccgttcc atcgttgccg gcggagatga gcgggttcaa accataagcg gtttgcatgg
     3721 ccgcaggttc atatccacag ggaggatagt gcccggcgcc accgtgaatg tcgccaccat
     3781 agcggttgcc actgtagtag gcagccggca gatgaccact cgtcgtgaag gcatgggttt
     3841 cgacgccgcg atagcattga ttttcataaa agaggctcgg ggccggcccg gttaaggggc
     3901 ccttctgagc ggttgcgccc agtggcattt caggaaatgg ctgaccggtc tcgggatcga
     3961 ccggacgcac cgagcggggc ttcatcgcta ccggatgcag tccctgcacc gaactaacaa
     4021 aagctccagc aggtccggcg acggaagcat tcgatgtatt ggagtaaatc gttttgccat
     4081 tcacattgaa tcggttgatc tgtacgttga aggccttctg cacgtctgcg acgctgccct
     4141 gtgcattcac atagaagtga ttctgatggg ccgaggtaac cactaaatta tgggaagcga
     4201 ggaagtcttt gactaccgtt gcttcctggg ccgccgggcc gaacctggcg gcatactgcg
     4261 ccggggtcag ccaatgctga tacaacggag acttcgggtc gtagagctgc ttcagtagtg
     4321 catcgcgttc ggcgatgttg tgctcctgca accgtaccgt gatgttgatg actttcgaag
     4381 ggctctctgg tccgaggttc tgtgcactct gcacgaaccg cggcgtgctg ttggcgagta
     4441 cctggccgtt ggcggcgaac gccgagccgg ccactgtgag catcagcaga atggcaacgc
     4501 caataagcgc gccagtccgc cagcaatcat gacgtatgga catgatgaat cattctcctc
     4561 taggctacgc agcccgcgcc gatcggcgca tcaagctgct ggataatcgt ggggccgtta
     4621 gccggcaacc gccggccatt cccgctagac ggcgagtggc aattccggct tacatcgtca
     4681 atttgcggcg caccacgccg gctagaccaa ttaagccgga gccaagcagc aggatgctgg
     4741 agggttccgg agtggtggag aagaacgcgg tgcttgcccc accattggtg gtaaggaccg
     4801 agaaccactc cgaccctgca cctccgggag cagtctgggc caggacaatg ctcaagttat
     4861 agctgccggc gccataaatc ggggcgaaga aagcagtacc tgtaacggtg tacagcgtgc
     4921 tgcagtagtt gcagtcctgc agtaggaagt acccgacgaa gttgccgttg atgtaaacgt
     4981 cgttctcgta agtcgcaccg gggattccgc caaagtcatc cacgacaccc caattagctt
     5041 caagattatt cacccagggc accgtggaaa aaaacgtctc ggtgatgaaa tcaccggccg
     5101 tccacatggg cttcgttaga ccaccgttgt tgcccatgaa atcgcacggc ggattggtct
     5161 ggctgcagta gaaagtggtg ttgctgggaa acggcacgcc gaatccgctg ttggcaaatg
     5221 cgccgggggc cagaatgcat agcgccaaaa cagtcatagc gctaaggtat gtaattcttc
     5281 tcatgaggat ctcctttgat tggcgcggag gaaaggtcaa taagttccgc gaacgaagcg
     5341 tatatgacgt aggtcaggaa tactagtaat ttcactgcat tatcggaggt ttttcccatt
     5401 tttaaaccta tcgcagtagt agttgccatc ggtacgaagg ctacaaaatg tcgtgcaatg
     5461 gccccgctca tgctgaagac agccgccaat cccgctggcg tgccgataga cgccttcgat
     5521 acgctgcgct ccgcagtcca agccgaccgc tcgcagttct tcgaagacct cagcagacgg
     5581 cggagacggc tacttaagat aggcccgcac aatagccagc aaatcgtctg tgacttcttc
     5641 agaagccttc cctcggccca ggtgttcgcg gatgtgctcc tccataacct cggccataag
     5701 gccgttgatc gctccgcggc aggccgccag cagttgcagc gtgtccgcgc actcctcttc
     5761 ctcgttcgac aaggcgcgtt ctaccgcctc gatctggcca cgaattttcc tcactcggct
     5821 gagttgcttg tcgcgattgc gatgaacgat cttcttcgtc ataaagcttg cctataccct
     5881 accccagtat gctataaact ttatggtgat gcaatcacgg ctatgcaccc tttcgcccca
     5941 gcgaaggctg taaccagcct gtaacaaaat cgtgtgacac tgaagtgatt gccgtttgcg
     6001 catgaatctt gttcagcggc agaaggcaag tgggagactt atcaagtgag acaggccagt
     6061 ccaattcggt ccgactcatg ggcggcagcc gtcggtctcg cgaggtcgac gctgctccgc
     6121 accatcggcg cattgctctg cctcgccgcc ctgtccatag ctacacgggc acaggaccca
     6181 cctgcggacg ccggtacccc ggacgcccca acgccacgtg acacattctt cgagcattcc
     6241 aacacggcga agtggtgggt ctcgggacag gccaactttg tgtttcaggc gcacggagat
     6301 ttctatgccg cctacagcgg taccaacagc ctgcacgaca ccgcggaaaa cgccacttcg
     6361 cgcgtactca ctctctttac gggatacgaa ttcaccccga gttccattct ttatctcgac
     6421 gtcgaagagg caggatgggg aggcatcagc ggcgctcttg gcttggcggg attcaccaac
     6481 ctcgatgtag tgcgtaatcc tcaactcggc acccagccct atatcgcgcg gctgatgttc
     6541 cagcagatca ttcccttttc caaggagaag atcgaaatcc cgcgtggtcc gcttactctg
     6601 gccactcaac ttccagcgcg gcgtctggaa attcgcttcg gcaaattctc catggccgat
     6661 tttttcgaca ataacgtcgg cggatccgac agccattacc agttcttgaa ttggaccgcc
     6721 gccaacaacg gcgcatggga ctacgccgcc gacacccgtg gttacaccgt gggagcgatt
     6781 ttcgattatg aagatcgcaa ctggggtctg cgcttcgcgg aggccctgat gcccaaggtc
     6841 gccaatggcc caaaccttga cgctgacgtg gcgcgggcgc gcgccgaaaa cgtcgagttg
     6901 gagctgcgct acaagctgct gaagaaaaaa gatacgtcag tccgtttcct cagctacgtc
     6961 aaccacgcca acatgggcac gtatcgcgac gccatcgcga tctacctcgc cgaccccacg
     7021 caaccgcccg atatcatcgc cacccgcaag caggggacga ttaagtacgg cttcggcatc
     7081 aactttcagc aggaattctc cagcgacgtc tgggccttcg ggcgctgggg gtggaatgaa
     7141 ggccagcacg agtcgttcgc ctacaccgag gctgatagca ccctccagtt gggcgtctat
     7201 gccaagggaa cacgctggca tcgcagcctc gatcgcctgg gcgcaacctt catctccaac
     7261 ggaatcggcg cttaccatca gacctaccta gccgatggcg gcttgggatt catcctcggc
     7321 gacggcggac tgaactacgg tcgcgaaacc atcgaggaag cgttctatac cattcacgcc
//
LOCUS       DASRKK010000185        16991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_12778, whole genome shotgun sequence.
ACCESSION   DASRKK010000185 DASRKK010000000
VERSION     DASRKK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16991
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12778"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..503)
                     /locus_tag="VFC15_04070"
     CDS             complement(93..503)
                     /locus_tag="VFC15_04070"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZM09367.1"
                     /translation="MESTPQRISSYKYLIVWQKALDLVTEVYAATSGFPRKEMFGLTS
                     QLRRSAVPVPSNVAEGQGRATKGEFIQFLSPARGSLYELETQIVIAGRLGYLLAEREH
                     ALAARAEEVARILNGLLTSLGVASRKLSTVDAPR"
     gene            complement(532..2007)
                     /gene="trpE"
                     /locus_tag="VFC15_04075"
     CDS             complement(532..2007)
                     /gene="trpE"
                     /locus_tag="VFC15_04075"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZM09368.1"
                     /translation="MVTPDYKSFLQLSRDATLIPVAKTLSADLLTPVAAFLSVAARQK
                     YAFLLESVEGGEKIGRYTFVGAQPRMLLTARGQNLTIRRGSDEEHRTGNVNDVLRETL
                     RQFRPAAVPGLPPFTSGGVGYFAYDMVRQFERLPETAKADVDLPDCIFTFYDRLLAFD
                     HLKHQLHIIAAADVRTESPRKAYDRALADIDALERKLVRGIHKQELGWSAPAKRKLIK
                     VHTSTTHDEFLASVRRAKEYIAAGDIFQVVLSQRLDFKPRAEPFQIYRALRTVNPSPY
                     MFFLQFDDLHVVGASPEMLVRITGRKLEYRPIAGTRRRGEEVADDERLIEELRSDEKE
                     RAEHVMLVDLGRNDLGRVSDYGTVQVRDLMYVERYSHVMHLVSALEGQLRSPLDALDA
                     VAACFPAGTLSGAPKVRAMEIIEELEPVRRGVYGGAILYADYAGNLDSCIAIRTMVLH
                     RGHAYVQAGAGIVADSDPESEYQECLNKASALLRAAERAGG"
     gene            2337..4940
                     /locus_tag="VFC15_04080"
     CDS             2337..4940
                     /locus_tag="VFC15_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM09369.1"
                     /translation="MQPLIQSWRSYLRSGRPTWFAGLYIAFSWVWIFLTDKLIEHFSF
                     TMATAMRWGTVKDCLYIAITSGLIYAILQRLNETNNNLENTVTSRTAALAASEAESRS
                     REEWLQRLLASLPDVSWSSAEDGRTIYISPNVEELFGYSPEELCEFGEEYWLQTVHPD
                     DLQRVAESYRALFSRGQPFDEQFRAKCKDGHWLWVHDRAVRTHMEDGILCADGILSDI
                     TARKDAELARIESEQRYRLLFERNLAGIFRAEVGGNLLDCNPALVRMLGYHSADELLG
                     RPSAEILYDPAEQRMLLENLARSGAISNVEIRLRRKDSAILWGLHNVNLLAPENGGPP
                     CIEGMVIDVTERKQTVSALSQQLSLMQAITSTAPNGLLLLDAEGRLTFINPAGERMFG
                     YTAAELHGKVMHDVCHYKHPDGSPFPRSECPIAQAGASGKTLQGYEDVHFRKDGAPVD
                     ISFSSAPLLEGGRVVGSVLVAQDITQRKLAEQKYQSLQEQFLHAQKMEAIGCLAGGIA
                     HDFNNLLQVINGYSILIVDECGPNPEIAKRAWAIHETGTRAAQLTQQLLDFSRKEQGH
                     TQTISLGSAVNKLMKLVRTLVGENIDLTAANRTNGACARINPGQLEQMVMNLVVNSRD
                     AMPKGGKLHIETSCVNLDEYSAMAFGNLSHGAYVQLSVSDTGCGMDAETMSRAFEPFF
                     TTKEPGKGTGLGLSTVYGIATQNGGGVRIDSAPGAGSTFHVCLPVAESEPARPVIQLR
                     LALPQGTESILLAEDEAGVRSLISGQLGKLGYKVTEAGNGAEALQLAKDAAHSIDLLI
                     SDVMMPKMGGKELAERIREICPAIKIVQMSGYHDMPQTPPDDGANSIQHLQKPFDLET
                     LAATVRHALDQ"
     gene            4962..5342
                     /locus_tag="VFC15_04085"
     CDS             4962..5342
                     /locus_tag="VFC15_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZM09370.1"
                     /translation="MTTVRMDKWLWAARFFKTRALAARACELGRILSHGQPAKPAREV
                     RVGDLLQVTNDGGDFQVEVLILSEMRGPAPVAQTLYRETDESRELRAKLADERKAIGQ
                     FETSPEGRPSKRDRREINKLRGRV"
     gene            5516..6784
                     /locus_tag="VFC15_04090"
     CDS             5516..6784
                     /locus_tag="VFC15_04090"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZM09371.1"
                     /translation="METTLTLEQEKNPWESQAARFNVAAEKLKLDPGLWRVLQQPNRE
                     IIVHIPVMMDSGQLEVFTGYRVQHSIARGPAKGGVRYAPDVSLDEVRALASWMTWKCA
                     VVNIPFGGAKGGIICDPKHMSRGELERMTRRYTSELIEFIGPEKDVPAPDVNTNEQTM
                     AWMMDTYSMHMRQTVTAVVTGKPLNMGGSRGRREATGRGVMIIADESIKYLKMARETT
                     RVIIQGFGNVGSNAALLMHEAGYKVIGIGEWDGGLYNSKGIDIAALTEYRQKEGTIHG
                     FKGAERANTDELLITDCDVLVPAATEAVITSLNADKVKAKILVEGANGPTTAVADEIL
                     ADKHVFVMPDILANAGGVTASYFEWVQDRQGYFWKESVVNEQLKDIMVESFEAVVSFA
                     EKHQVNNRIAAYMLAIDRVAFTIRQRGIYA"
     gene            7215..7490
                     /locus_tag="VFC15_04095"
     CDS             7215..7490
                     /locus_tag="VFC15_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09372.1"
                     /translation="MGNAQSKLNMHRLTFTLRLLAVVVSSPVIVLVIAFATLLHPITS
                     LKTKFHTSLRIWMSVMDWIKGIPIGYTYWVQYHAPSMLDEIAVHHEE"
     gene            7556..8245
                     /gene="pgsA"
                     /locus_tag="VFC15_04100"
     CDS             7556..8245
                     /gene="pgsA"
                     /locus_tag="VFC15_04100"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZM09373.1"
                     /translation="MARWGAALQSAFVNLPNSITLTRILSIPVLIWILSSSHFSSTNG
                     DRELLASAVFIAASLTDAIDGYLARKRAQVTTIGMLLDPVADKLLIAAAFITLVQFNP
                     RTVPAWMAVIIIGREFLVSGLRSIAASEGFTIEASDLGKMKMLVQIVSVVACIVDHHW
                     TRLPWYFLSLPVHPIALASIWIMVGLSLVSAADYFLAFWNKISQHSEKRRKRRSFVLS
                     RRRKRNDVPVV"
     gene            8256..8831
                     /gene="amrA"
                     /locus_tag="VFC15_04105"
     CDS             8256..8831
                     /gene="amrA"
                     /locus_tag="VFC15_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZM09374.1"
                     /translation="MTLATSAEYTQEERELLLQLAHRSIQAVLDCRGLDLTSPSAHLA
                     EMRGAFTTLHLEGRLRGCIGYVVPTNSLYQTVAETARAAAFDDPRFPPVTPDEAPQLK
                     VEISVLSRLHPVRPENVVTGVHGLIVAKGGRRGLLLPQVPVEWHWDRETFLSQTCLKA
                     GLPPDAWMQDIDLQAFTAEVFGEESPAERSE"
     gene            8951..10669
                     /gene="aroE"
                     /locus_tag="VFC15_04110"
     CDS             8951..10669
                     /gene="aroE"
                     /locus_tag="VFC15_04110"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZM09375.1"
                     /translation="MQVSTTIFASRIQRFRLPRVCVAVVGEDATNIIDRAESLVRDNP
                     FMEFRLDYLTNPAAGLPKLKHFLDLHPEATVIATCRRAVNGGKFKGSASAQLDILTKA
                     AATGFQLVDIELQSAEILKAAELTELKDRVGVILSFHDFKATKKLEETFAEMKRYPAD
                     FYKIVSTATSLYDNVQMMKFLQAHSAQHEMVGLCMGEQGIISRVLGVRAGSVFTFGAA
                     TRGEETAPGQVTAGELRDTYRIEMVDAATQVYGVAGDPVAHSMSPIMMNAAFRRETVN
                     AVFLALHAKSMKDLMACVPDIPIRGLSITMPYKQDIVAALTNSDALTKQVGACNTVVR
                     GQDGKLYGFNTDVAGVVVPLEQRMTLQGAKILVVGAGGVARAAAFGLKNKGAEVFIVN
                     RTPEKGQALAKATKAKYLKRADVAKMSFDVIINATPLGMGANRQSPLEDKELNTKFLF
                     DLVYVPAETKLVKMARARNIQVIPGLEMFVQQGARQFEIWSGKPAPIAEMSFVVTKAL
                     ERLGVAPVEEELPAPKARPRDEEKPAPKPLAKPAVKSSSKKAARPAPTKPRKPAKKTA
                     KKPVKR"
     gene            10997..11734
                     /locus_tag="VFC15_04115"
     CDS             10997..11734
                     /locus_tag="VFC15_04115"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZM09376.1"
                     /translation="MAWYAYCITEQSGLQGNSRARRPFALENLKGVNDVPVLAYPSGD
                     FAVIVSEHSPAQTLTQQAIVDHARVISDCFRTLTVLPFRFGTIFESDEALRRAVRGNR
                     KAFLESVARLRGKSEMHVKILVKDGALQEAITAMPVGVGGEYLRKLRDHASRDRERQT
                     KARALSMQVHKLFCPLEEDVVCKRTEAGKMLMDFAHLIDTSTVTKYQNRYSAATRHFK
                     DCQVTITGPWPPYHFMPGKLRTVSGGN"
     gene            12074..12442
                     /locus_tag="VFC15_04120"
     CDS             12074..12442
                     /locus_tag="VFC15_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZM09377.1"
                     /translation="MDKKKLDAFKKRLEERQRELRFNVSRTAHDGREADLESAQDVAD
                     RAANSYTKEFLFSQSNSERMTLQMVDGALDRIREGSFGECIACGTEINPKRLEAVPWT
                     RHCIECQEKVEQGLLEATGT"
     gene            complement(12523..15348)
                     /locus_tag="VFC15_04125"
     CDS             complement(12523..15348)
                     /locus_tag="VFC15_04125"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZM09378.1"
                     /translation="MRKILIGVVAVIVLVVVGMLLLPHLIDVNQYRGEIQARFQSRLN
                     RPVTLGAMDLAVFPLRVQVQNVSIGEDPRYHSTVPFADVGELDISVKLFPLLSKTIAI
                     ESLTLKRPKIELIKDQAGVWNFASLGEVPAPVTNNAAAPTQPPAQAKPPAATPTPAAS
                     DGGSFALDKLAISDGQVAVTDFQKKQPRAVYDHIDLTLKDYAPNQPFSVDLAAHVPGK
                     GAETLSLSGKGGPINQAQMLSTPFDGKLKLNEVSLAGAQKFLNNAALEGTDAMLSGST
                     DLTNAGSKMSAKGSLKITDAVIHNVQVGYPIEAEFNVTDDLSSDVIQIGKGDVKLGST
                     PLTLTGTLNTHAATTVADVNVSASDASIEDAARLAAAFGVAFSPNAKITGKLTANVHA
                     QGPTDHLALHGTVNGHNLEVTGKDIPSAVKVPALDLNMTPQDIRSNPFTVTSGATTLS
                     GQMSIAQYTSASPTVDATFKTVNAKLDELLNIAKAYGVSAVEGMSGSGAVNIDVHATG
                     PIKNTDAMNFSGTGAIQNASLKMPTLTQPLNIKNANLQFTQNSLSVTNLAASLGSTNA
                     SGNLSVANFQAPRLTFALAADKINITELQKLMVAGKPAPAKKAEASWSLVPSADAAPA
                     PQPSFLDTATGTGTLTVGSLTYDRTTLTNVHSNVNLNHGVIQLNPLTGQVFGGQISGS
                     ITADLRHETSSFAVNAKLSGADANQLLTAVANTKDSVYGTLNATMNQTFSTPASGDVT
                     QTLNGPFAFTLTNGKLTKIDLVSELGKIGKFGTTGKGYTTVSSMSGTFDVRNGVANTN
                     DLKAALDVGNMAATGSMNLVTEALAMHLTAVLNKSFSQSVGGAGVGGYLTTALGNKNG
                     ELVLPVIIGGTMSKPVVTPDVQKIAEMKLNNIVPGASGILGSLLGGKSQPQGQQAGKP
                     AAQPSQQQQLQDALGGLLGGSKKKPPK"
     gene            complement(15533..>16991)
                     /locus_tag="VFC15_04130"
     CDS             complement(15533..>16991)
                     /locus_tag="VFC15_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM09379.1"
                     /translation="VLIVDDEKLVRWSLRQKCEEWGYHVLEADNGAAALRVAHNESPD
                     LVLLDVRLPDASGIAILEQIKKAGDARAIIMITADPQLEDVKTALKLGAYDFVGKPLD
                     FDELAVAVKNALETTRLRTEVESLRGEVRRRAGYHNVIGVSEKMAELLGFVNKVAASE
                     ATTVLIQGESGTGKDLIAKAIHYQSRRQDKPFVAINCSAIPETLMEAELFGHERGAFT
                     DAKAMKKGMFEMADGGTLFLDEIGELSPLLQAKLLRVLEDQVIRRVGGVRDMQVDVRV
                     IAASNRDLERGVRENTFRQDLYYRLAIISIFLPPLRERKEDILPLVEYFIDRYNRKFR
                     KTVQGITDESRRLMLKYDWPGNVRELKNAVERAMILEEGNVLRPDYLPFAVAQQQASY
                     TAFEMSSGNSSGNGGGVTQLPDGRSLPRLSIPEGGTSLEEVERALVEMAMTRANGNQT
                     HAARLLDISRDALRYKLKKFGLIHAEEDETSNTAS"
BASE COUNT         3536 a         5012 c         4954 g         3489 t
ORIGIN      
        1 ttctcaatca ctttcacccg ttggaactct ttctgactca tcatcaccat ccttcgaggg
       61 tgacaaagtc tcgttgcagt tagggggtga cattatcttg gagcatctac agtggatagt
      121 ttacgactcg cgacgcccaa cgatgtaagc aggccattca aaatgcgcgc gacctcttcg
      181 gcccttgcag caagcgcatg ttcgcgctcc gctagaagat atcccaaccg tcctgcaatg
      241 acgatttgcg tttccaattc gtacagtgac ccccgggcgg gtgacaaaaa ttgtatgaac
      301 tcgcccttcg tggcgcgtcc ctgaccctcg gcgacgttgc ttgggacggg cactgctgag
      361 cggcgcagct gggatgtcag tccaaacatc tctttgcgcg gaaacccaga cgtcgcggcg
      421 taaacctcag tcaccagatc cagggccttc tgccacacga tgagatattt ataactgcta
      481 attctttgag gggtcgattc cataacagtc tccgcaactg accactaacc actaaccacc
      541 agccctttcc gccgcccgaa gcaacgcact cgccttattc aggcactcct ggtattcact
      601 ctcgggatcg gaatccgcga cgatgcccgc gcccgcctgc acgtaggcgt gcccacgatg
      661 cagaaccatg gttcgaatag ctatgcagga atcgaggttt cccgcgtaat ccgcataaag
      721 aatcgctccg ccataaacgc cgcggcgcac cggctccagc tcttcgatga tctccatggc
      781 gcgaactttc ggagcgccgc tcagcgtccc ggcagggaag catgccgcca cggcgtcaag
      841 cgcatccagc ggactgcgca attgtccttc cagcgccgac accaggtgca tcacgtgaga
      901 gtagcgctcc acatacatca ggtcgcggac ctgcaccgtg ccatagtcgc tgacgcgtcc
      961 cagatcgtta cgcccgagat cgaccagcat cacgtgttcg gcgcgttcct tctcgtcgct
     1021 gcgcaactct tcgatgaggc gctcgtcatc ggcaacttcc tcgcctcggc ggcgtgttcc
     1081 cgcgatgggt cggtactcca gcttgcgccc agtgatgcgc accagcattt ccggcgaagc
     1141 cccaaccacg tgcaagtcat caaactgcag aaagaacatg tagggcgaag ggttgaccgt
     1201 gcgcaaagcg cgataaatct ggaatggctc agcccgtggc ttgaagtcga ggcgctgcga
     1261 aagcacaacc tggaaaatgt cacccgcggc aatgtattcc ttggcacgcc gaacgctagc
     1321 caggaattcg tcgtgggtag tgctggtgtg taccttgatg agcttacgtt tggctggggc
     1381 cgaccagccc agctcctgct tatgaatacc gcgcaccagc ttgcgttcca gcgcatcgat
     1441 gtcagcgagg gcacggtcgt atgccttgcg cggcgattcg gtacgaacat cggcggcggc
     1501 gatgatgtgc aactggtgtt tcagatgatc gaaggccagt aggcggtcgt aaaaggtgaa
     1561 gatgcaatcc ggcagatcga catcggcctt ggccgtctcg ggaagccgct cgaactgccg
     1621 caccatgtcg taggcaaaat agccgacacc acccgaggtg aacggcggca ggccgggaac
     1681 cgccgcggga cgaaactgcc ttagagtttc gcgcagcacg tcgttgacat tgccggtgcg
     1741 gtgttcctcg tccgagcctc ggcggatcgt caggttctgg ccacgggcgg ttagtaacat
     1801 gcggggttgc gcgccgacaa aggtgtagcg accgatcttc tcgccgccct cgaccgactc
     1861 cagcaggaag gcatatttct gccgggcggc aacgctcagg aacgccgcaa ccggcgtcag
     1921 caagtcggcg ctcagcgtct tggccacggg aatcaaagtg gcgtcgcggc taagctgcag
     1981 gaaactcttg tagtcgggcg taaccatcgg gtccctggcg ctggttgcgg ggttgactgg
     2041 gattatacga gaggccggag gcctcctgtt gttgatgcgc caagataccg gataaggagg
     2101 ccgggaccat tgctttcgct ctttcggtcc aatccgtcac aatgacttgt gctgcaggtc
     2161 accaacaatg gagtaccgta acgttactat catcccaatt cggcggcccg aggttacaat
     2221 gggaaccgtc gaccttgcgg tagatctgtt gcgcccgccg catacgccga tgtacttcgt
     2281 gggggccgct cgacgtttcc tgatactgca agttttgtca ggtgaaggag aactggatgc
     2341 agccactcat tcagtcttgg cgtagttatc ttcgatctgg ccgaccgact tggttcgctg
     2401 gcctgtacat cgcgttttcc tgggtgtgga tcttcctcac cgataagctc attgaacatt
     2461 tctcgtttac gatggcgacc gcgatgcgct ggggcacagt caaggactgt ctctacatcg
     2521 ccatcaccag tggcctgatc tacgcgatcc tgcagcggct caacgaaacc aacaacaatc
     2581 tcgaaaacac ggttaccagt cgcaccgccg ctctggccgc gtcagaagca gagtcgcgtt
     2641 cgcgtgagga gtggctacag aggctgctag ccagcctgcc ggatgtgtct tggagctcgg
     2701 cggaagacgg acgcaccatt tacattagcc ccaatgtcga agaactcttc ggttattcgc
     2761 cagaagaact ctgcgagttc ggcgaagaat attggctcca gaccgttcat cccgacgatc
     2821 tgcaacgcgt tgccgagagt taccgtgcgc tcttttcgcg cggccagccg ttcgatgagc
     2881 aatttcgggc caagtgcaaa gacggccact ggctctgggt gcacgaccgc gcagtccgga
     2941 ctcacatgga agacggcatc ctgtgcgccg acggcattct ctcggatatc accgcgcgca
     3001 aggatgcgga actcgcgcgg attgaatccg agcaacggta ccggctgctt tttgaaagga
     3061 acctcgccgg aattttccgg gcggaagtgg gcggaaacct gctcgactgc aacccagcgc
     3121 tggtccgcat gcttgggtac cactcagcgg acgagctatt gggtcgcccc tcggcggaga
     3181 tcttgtacga cccagcggaa caaagaatgt tgctggagaa tctggccagg agcggcgcca
     3241 tcagcaatgt cgagataagg ctcaggcgga aggacagcgc gattctttgg ggattgcaca
     3301 acgtcaacct gctggccccg gagaatggag ggccgccatg catcgagggc atggtgattg
     3361 acgtcaccga acgcaaacag acggtgagcg cactgagcca gcaactttct ttgatgcagg
     3421 ccatcacctc gacggcgccc aacggcttgc ttctgctgga tgcggaaggc cgcctgactt
     3481 tcataaatcc cgccggcgaa aggatgttcg gctacaccgc ggccgaactg catggcaaag
     3541 ttatgcatga cgtctgccat tacaagcacc cagatggcag tccgttccct aggtcggaat
     3601 gcccaatcgc gcaggcaggc gcgtctggca agaccttgca gggctatgag gacgtgcatt
     3661 ttcgcaaaga tggcgccccc gtcgatattt cattttccag tgcacctttg ttggaaggtg
     3721 gccgcgtcgt gggttccgta ctggtggcgc aggacatcac ccagcgcaaa ctggcggagc
     3781 aaaagtacca atcgttgcag gagcagttct tgcatgctca gaagatggag gccattgggt
     3841 gtttggcggg aggcattgcg cacgatttca acaatctgct gcaagtcatc aatggctatt
     3901 cgattttgat cgttgacgaa tgcggcccca atcccgaaat tgccaaaaga gcatgggcta
     3961 tccacgaaac ggggacccgc gccgcccaac tcacccaaca gcttctggat ttcagccgca
     4021 aagagcaagg ccatactcag accatttccc tgggcagcgc cgtgaataaa ctgatgaagc
     4081 tggtgcggac actggttggc gaaaacatcg acctgactgc tgccaaccgt accaacggtg
     4141 cgtgtgccag gatcaatccc ggacagttgg agcagatggt catgaacctg gtggtcaact
     4201 cccgcgacgc catgccaaag ggcggcaagc tccacatcga gaccagttgc gtcaacctgg
     4261 atgagtattc agcgatggcc ttcggcaacc tctcgcacgg ggcctacgtg caactttccg
     4321 tgtccgacac cggatgcggt atggatgcag agaccatgag ccgcgccttc gaaccgtttt
     4381 ttaccaccaa agaacctggc aaaggcacag gattgggatt gtctacggtg tacggtatcg
     4441 cgactcaaaa cggcggagga gttcgtatcg acagcgcgcc cggtgcagga tctactttcc
     4501 acgtttgcct gcctgtggct gagtcagaac ccgcgcgacc ggtgatccag ctgcggctgg
     4561 cgctgccaca ggggacggaa agcattctgc tggcggaaga cgaagccggc gtccgatcgc
     4621 tgattagcgg ccagctcgga aaacttggct acaaagtgac ggaagccggc aacggcgcag
     4681 aggccctgca gctggcgaag gacgccgcgc actccatcga cctgctcatc agcgacgtaa
     4741 tgatgccgaa aatgggagga aaagagctgg cggagaggat ccgcgaaatt tgtccagcca
     4801 tcaagatcgt ccagatgtcg ggataccacg acatgcccca aacgccgccc gatgacggag
     4861 cgaacagtat tcagcatctc cagaagcctt ttgaccttga aaccctggct gcgactgtga
     4921 gacacgcgct cgatcaatag tggcattatc agacaaagac catgaccacc gtacgtatgg
     4981 acaagtggct gtgggccgcc agatttttca agactcgcgc cctggctgca cgggcctgtg
     5041 aactcggcag gattctctcc cacggacagc cggccaagcc tgcgcgcgag gtccgcgtgg
     5101 gagacttact ccaggtgacc aatgatggcg gcgacttcca ggtggaggtc ctgatcctca
     5161 gcgagatgcg cggccccgcc ccggtcgcac agacgctgta tcgcgagacg gacgagagcc
     5221 gtgaattgcg cgcgaagctg gcagatgagc ggaaagcgat agggcagttt gaaacctcgc
     5281 cggagggcag accgtcgaaa cgcgatcgcc gcgaaatcaa taagctcagg ggtagagtgt
     5341 gaccgcggat aactaacggg gaacccggcg cttcgagacc gcggttgtgc ttgcgccctt
     5401 tccgcagccg tgcatatact cgtaaggttt gcccgcctga cgatcgaata gcgcgtcttg
     5461 cggactttag aatggctcgg gccgcagcag ccccggcaat cttcggagag attttatgga
     5521 aaccacactc acgctcgaac aggaaaaaaa tccctgggaa tcacaagcag cgcgtttcaa
     5581 cgtcgcggct gaaaagttga agctcgatcc cggtctgtgg cgcgttctgc agcagcccaa
     5641 tcgcgaaatc atcgtacaca tcccggtcat gatggacagc ggacagctcg aagtgttcac
     5701 cggctatcgt gtgcagcact ccatcgctcg cggtccggcc aagggcggag ttcgctatgc
     5761 tcccgacgtt tcgctcgacg aagtgcgcgc actggccagt tggatgacct ggaagtgcgc
     5821 ggtggtgaac attccgttcg gtggcgcaaa gggtggtatc atctgcgatc ccaagcacat
     5881 gtcgcgtggc gaactggagc gtatgacccg ccgctacacc tccgagctga ttgaattcat
     5941 cgggcctgag aaggatgttc ccgcccccga cgtgaacacc aacgaacaaa ccatggcgtg
     6001 gatgatggac acctactcca tgcacatgcg gcagacggtc accgccgtgg tcaccggaaa
     6061 gccgctgaac atgggcgggt cgcgcggacg ccgcgaagcc acgggccgcg gcgtgatgat
     6121 cattgccgac gagtcgatca agtacctgaa gatggcgcgc gaaaccacgc gggtcatcat
     6181 ccagggcttt ggcaacgtcg gatcgaacgc cgccctgctg atgcacgaag ctggttacaa
     6241 ggtcatcggt atcggtgagt gggatggcgg cctctacaac agcaagggaa tagacatcgc
     6301 cgcgctgacc gagtatcgcc agaaggaagg taccatccac ggatttaagg gcgcagagcg
     6361 cgccaatacc gatgagctgc tgatcaccga ttgcgacgtc ctggttcccg ccgccacaga
     6421 ggccgtcatc acctctctca acgccgacaa agtgaaggcg aagatcctgg tggagggcgc
     6481 gaatggcccg actacagcgg tggcggacga gatcctcgcc gacaaacacg tgttcgtcat
     6541 gcctgacatt ctggccaacg ccggcggcgt gactgcttcc tacttcgagt gggtgcagga
     6601 tcgacaggga tacttctgga aagagtcggt agttaacgag caactgaagg acatcatggt
     6661 cgagtcgttc gaagcggtgg ttagcttcgc tgagaagcac caagtgaaca accgcattgc
     6721 agcttacatg ctggccatcg accgcgtggc gttcaccatc cgccagcgcg gaatctacgc
     6781 ctagcggctg ggttagcagg ctacaacggc accccgcgtc gggagccgtc tttattttgc
     6841 tgcaagcctt cggccatttc gtgcgcttca agaagccgag gcacggaggc ggaacgaatg
     6901 cgtcgttctt cttcgtgcct tcggagctgc aggttgatca cgccacgtgg gatcagaaat
     6961 ccactttgat gcgagtcgcc gtcagtgtgt cgctgtcgta ttcggcgacc agatttccat
     7021 tcaagtcgtg cacgtaaaca ttttcgctac cggtcgccca aaatacccag aaaaactgaa
     7081 accgcctctc gagcgaactt ttttcgatgc tcccgtgctg gtggatggcg caaatttacg
     7141 cgcaaagaaa atttacatac atggcatgcc gcaaccgccg gaatgggagc ataatatttt
     7201 gcaaggaagt ggccgtgggg aacgcccaat ccaaactcaa catgcatcgc ctgactttca
     7261 ccctccgttt gcttgcggtg gtggtgagct cgccagtcat cgtgctggtc atcgccttcg
     7321 caaccctgct gcatcccatt acctcgctga agaccaaatt ccacacatct ctgcgcatct
     7381 ggatgagcgt gatggactgg atcaagggca tccccattgg ctacacctac tgggtgcaat
     7441 accacgcgcc cagtatgctg gacgaaatcg cggtacacca cgaagaatag gcccgcaagc
     7501 ccctctcttg ggcggttgct ttcatgccga gcagtggacg ataatcacct tggcagtggc
     7561 aagatggggc gccgcgctcc agtccgcttt tgtgaacctt ccaaactcca tcacgcttac
     7621 gcggattctc agcattcccg tgctgatctg gatcttgtcg agcagtcatt tcagctccac
     7681 taatggcgac cgggaactgc tggcgtccgc ggtcttcatt gcggcttcgc tcaccgatgc
     7741 catcgacgga tacctggcac gcaaacgcgc ccaggtcacc accattggaa tgctgctcga
     7801 cccggtggcc gacaagcttc tgattgcggc cgccttcatc acgctggtcc agttcaatcc
     7861 ccgcacagtg cccgcctgga tggcggtcat cattattggc cgcgaattcc tggtcagcgg
     7921 gttgcgctcg atcgccgcct ccgagggctt caccatcgag gccagcgatc tgggcaaaat
     7981 gaaaatgctg gtgcagattg tgtccgtggt cgcctgcatc gttgaccatc actggacgcg
     8041 cctgccttgg tatttcctca gcctgccggt gcaccctatc gcgcttgcct cgatctggat
     8101 catggtgggg ctgtcgctgg tctccgccgc ggattacttt ttggcattct ggaacaagat
     8161 cagtcagcac tcggaaaagc gccgtaagcg tcgttccttt gtgctgagcc gacgcaggaa
     8221 gcgcaacgat gtccccgtgg tctgaaacac aacccgtgac cctggccaca agtgctgaat
     8281 atacccagga agagcgcgag ctattgctgc agttggccca tcgctcgatt caggcagtgc
     8341 ttgattgccg ggggcttgac ctgacctccc ccagcgcgca cctggccgag atgcggggcg
     8401 cgtttaccac cttacatctg gaaggacgcc tacgcggctg catcggatac gtcgttccca
     8461 caaactctct gtaccagact gtggcggaaa cggcacgagc tgcggccttc gatgaccctc
     8521 gcttcccccc ggtgacgccg gacgaagctc cccagctcaa agttgagatc agcgtgctct
     8581 cacgtcttca tcccgtccgg cccgagaatg ttgtgaccgg agttcacggc ctgatcgtgg
     8641 ccaaaggcgg ccgccgtggg ttattgctcc cgcaagtgcc ggtagagtgg cattgggacc
     8701 gcgaaacgtt cctctcacag acctgcctga aggctggttt gccacccgat gcttggatgc
     8761 aagatattga tctgcaagcc tttactgccg aagtgtttgg agaagagtct cccgcggaaa
     8821 ggtctgagta agaaccagcc gacacgcctg aaatgatctg tagtcctgcc tcgattgggc
     8881 aataaattca ccattccaca acaagacagt tgcaacattc gttgtgcatc tgtaccatcg
     8941 ggacgctcat atgcaagtct cgacaaccat tttcgcctcc cgtatccaac gtttccgtct
     9001 tccccgtgtc tgtgtcgccg tagtgggtga agacgcgacc aatattatcg atcgcgccga
     9061 gtctctggtg cgcgataacc cgtttatgga gttcaggtta gactacctca cgaaccccgc
     9121 tgcgggcctc cctaagctga agcactttct cgatttgcat cccgaagcga cggtgattgc
     9181 cacctgtcgc cgagcggtca atggcggcaa gttcaaaggc agcgcctccg cccaattgga
     9241 catcctgacc aaggccgccg ccaccggatt ccagctggtt gacatcgagt tgcaaagcgc
     9301 cgagatctta aaggccgccg agcttacgga gctcaaagat cgcgtgggag tgatcctctc
     9361 ctttcacgac ttcaaagcca ccaaaaagct ggaagagacc ttcgcagaga tgaagcgcta
     9421 tccggcggac ttctacaaaa tagtttcgac ggccaccagc ttgtacgaca acgtgcagat
     9481 gatgaagttt ctgcaagccc actccgctca gcacgagatg gtgggactgt gcatgggcga
     9541 acagggaata atcagccgcg tgctgggtgt acgcgcgggc agcgtgttca cattcggggc
     9601 tgcaacccgc ggagaggaaa ctgcgccggg ccaggtcaca gccggtgaat tgcgcgacac
     9661 ctatcgcatc gagatggtcg atgccgcaac ccaggtttat ggagttgcgg gcgacccggt
     9721 tgcacattcg atgtcgccga ttatgatgaa cgccgctttc cgccgcgaga ccgtcaatgc
     9781 ggtgtttcta gcgctccatg ccaagtccat gaaggacctg atggcgtgcg tccccgatat
     9841 tccgattcgt ggcctgagca tcaccatgcc ttacaagcag gacatcgtcg cggcgctgac
     9901 gaattccgat gcgctcacga aacaggtggg tgcttgcaat accgtggtcc gtggccagga
     9961 cggaaaactc tacggtttta ataccgacgt ggccggcgtg gtggttcccc tggaacagcg
    10021 catgactcta cagggcgcga agatcctggt ggtcggcgcg ggtggagtag cgcgcgccgc
    10081 cgccttcgga ctgaagaaca agggcgcgga agtgttcatc gtcaaccgca cccccgaaaa
    10141 gggacaggcg ctggcgaagg cgaccaaggc caagtacctg aagcgcgccg acgttgccaa
    10201 gatgagcttc gatgtgatca tcaacgccac gcccctgggc atgggagcca atcgccaatc
    10261 gccgctggaa gacaaggaat tgaacaccaa gttcctgttc gacctggtct acgtgccggc
    10321 ggaaactaag ctggtcaaaa tggcccgcgc caggaacatc caggtcatcc cgggattgga
    10381 aatgttcgtg cagcaaggcg cgcggcaatt cgaaatctgg agtggcaaac cggcgcccat
    10441 cgcggagatg agcttcgtgg tgaccaaggc actggagcgc ctgggagtag caccagtcga
    10501 agaggagctg ccagcgccga aggcgcggcc tcgcgacgag gagaaaccag cgccaaaacc
    10561 gcttgccaag ccggcagtga agagctcgag caaaaaggca gccaggcccg cacccacgaa
    10621 acccaggaaa cccgcgaaaa agacggccaa gaagccggtg aaacgctagt ttcaaacggt
    10681 ttcaacggct actgcgattt ttgcaaaact acttccgagg gtgcgctcca ccacaggagc
    10741 gcatctttgc gtttgccgaa aaccgccgtc cagcgcgggt ttcggagtac tctccaacac
    10801 ccgatttcga aaaaagcgcg gatttcctgc aattccttgt tgcagaatcg gtgcagatgg
    10861 gcttaggatg agaactggaa gcggttctct cccgcctcca ccttacaaaa cagacctaag
    10921 ccccacgttc gaagtcggaa aatccctcac gcgcagccgg tagaactgtg tatgagcttc
    10981 ccaggagaag acggagatgg cgtggtacgc gtactgtatt actgagcaat ccggcttgca
    11041 aggcaatagc cgggcccgac gcccatttgc gctcgagaac cttaagggcg taaacgatgt
    11101 tccagttcta gcctatccca gtggagattt cgcggttatt gtcagcgaac attctcccgc
    11161 tcaaaccctc actcaacaag ccatcgtcga tcacgcgcgc gtcatcagcg attgttttcg
    11221 caccttgacc gtgctccctt ttcgttttgg aaccatcttc gagtctgacg aagcactgcg
    11281 ccgtgcggtg cggggaaatc gcaaagcctt cctagaaagt gtggcccgcc tgcgaggcaa
    11341 gtccgaaatg cacgtcaaga tcctcgtgaa agacggagcc ctgcaggaag ccatcaccgc
    11401 gatgcccgtc ggcgttggcg gcgagtatct tcgcaagctg cgcgaccatg ccagccgcga
    11461 ccgcgagcgc cagaccaagg cccgggcgct ctccatgcag gtgcacaaac tcttctgtcc
    11521 cctggaagaa gatgtggtct gcaagcgcac tgaggccggc aagatgctta tggatttcgc
    11581 tcacctgatc gacaccagca ccgtcaccaa ataccagaac cgctactcgg cagctaccag
    11641 acatttcaag gactgccagg ttacgattac tggtccgtgg cctccctacc atttcatgcc
    11701 gggaaaacta cggaccgtgt cgggcggaaa ctagcttcga gaccacatga ttttttgaac
    11761 gacgaaggtg gctaaccggc cgccttttcc gtttgtgcgc gattcggcgt cgtgattccc
    11821 tgggactgaa gcggttgtgg cattctcacc ccatcgagct acgctgtagc gcgcctaacc
    11881 gtccgctcat ctcgccctac gccgttccca taaactacct cgattcccat cttgggaagg
    11941 aattcccagc gcacaaaatt agcctggacg gaaagttgac gcgtaccagc atggggcata
    12001 tgatctttca ttcgccaagc cgtaattttc gttcagggcg attcctgggc tggtgggcgt
    12061 ggggtgtgta gttatggaca agaagaagtt ggacgctttc aaaaaacggc tcgaagaacg
    12121 ccaacgggag ttgcggttca acgtgagtcg taccgcacat gatggccgcg aggccgatct
    12181 agaatcggcg caggacgtcg cggaccgcgc ggcaaactcc tacaccaagg aatttctgtt
    12241 cagccagagt aacagcgagc gcatgacgct gcagatggtt gacggcgcgc tcgatcgcat
    12301 ccgcgaaggc tcgttcggcg agtgcatcgc atgcggaacg gaaatcaatc ccaagcggct
    12361 cgaagcggtg ccgtggacgc gccattgcat cgagtgccag gaaaaggttg aacagggttt
    12421 gctggaggcg actggaacgt aaccgcgtcc gtcgtcgtcg agcaagagaa aaaagccgcc
    12481 cttgtgagcg gctttttgtc ctgcacgaaa ctcgccaaga gattatttcg gcggcttctt
    12541 cttgctgcca cccagcaagc cgcccagcgc atcctgcaac tgctgctgct gcgacggttg
    12601 cgcagccggt ttccccgcct gctgcccctg cggctgcgat ttcccgccca gcagcgagcc
    12661 gagaatcccg ctggcgccag gaacgatgtt gttcagcttc atctccgcga tcttctgcac
    12721 atcgggcgtc acgactggct tactcatggt tccgccgatg atgaccggca gcaccaactc
    12781 gccattcttg ttacccagcg cggtggtcag gtagccgcct actccagcac cacccaccga
    12841 ttggctgaat gatttattga gcaccgcggt gagatgcatc gcgagagctt cggtgaccag
    12901 gttcatgctt ccggtcgccg ccatgttgcc gacatcgagc gccgccttca agtcgttggt
    12961 attagcaacg ccgttcctca cgtcgaaggt gccgctcata ctggacacgg tcgtatagcc
    13021 ttttcctgtg gttccgaact tcccgatctt gcccaattcg ctcaccaggt caatcttggt
    13081 cagcttgcca ttggtgagag tgaaggcgaa gggtccgttc agggtttgcg tcacgtctcc
    13141 cgatgcggga gtcgagaagg tctggttcat ggtggcgttc agcgttccgt atacgctgtc
    13201 ttttgtgttg gcgacggcgg taagcaactg gttggcgtcg gcgccactga gcttggcgtt
    13261 gacggcgaag ctcgacgtct cgtggcgcaa gtcagccgta atgctgccac taatctgtcc
    13321 gccgaaaacc tgccccgtca gcgggtttaa ctggatgacg ccatggttca ggttgacgtt
    13381 ggagtgcacg ttcgtcaggg tggtgcggtc atacgtcagg ctgccgaccg tgagtgtgcc
    13441 agtcccggtc gccgtatcca gaaagcttgg ctgcggagca ggtgccgcat cagcggaggg
    13501 aaccaggctc cacgaggctt cagctttctt ggcgggggcg ggcttaccag caaccatcaa
    13561 cttctgcaac tccgtgatgt tgatcttgtc ggccgcgagc gcaaaggtga gccgcggagc
    13621 ctggaagttg gcgacactga gatttccact ggcattcgtc gatcccagcg acgccgcaag
    13681 attcgtcacg ctgagcgaat tctgcgtgaa ctgcaggttg gcgttcttaa tgttcagtgg
    13741 ctgggttagc gtcggcatct tcagcgacgc gttctggata gcgcccgtac cgctaaagtt
    13801 catcgcatcg gtgttcttga tcggaccggt cgcatgcacg tcgatgttga cggctccgct
    13861 gccgctcatg ccctcgaccg cgctcacacc ataagccttg gcgatgttca acagctcatc
    13921 cagcttcgca ttcacggttt tgaacgtggc atcgacagtc ggcgaggccg acgtgtactg
    13981 ggcaatgctc atctgcccgg agagcgtagt ggcgccggag gtcacggtaa acggattgga
    14041 gcggatgtcc tgcggcgtca tgttaaggtc gagcgccggc accttgactg ccgacggaat
    14101 gtccttgcca gtcacttcga ggttatggcc attcaccgtc ccgtgcagcg ccaagtggtc
    14161 ggtcgggcct tgtgcatgaa cattcgccgt aagttttccc gtgatcttcg cattgggact
    14221 gaacgcgacg ccaaatgcag ccgccagtcg cgctgcatct tcaatcgatg cgtcgctggc
    14281 ggatacgttg acgtccgcca ctgtggtcgc tgcgtgcgta ttcaacgtac cggtgagggt
    14341 cagcggagtc gatcctagct tcacgtcgcc cttgccgatc tggattacat cgctgctgag
    14401 atcgtcagtc acgttgaatt cggcctcgat cgggtagcca acctgaacgt tgtgaatgac
    14461 ggcgtcggtg attttcagcg aacccttggc tgacatcttg ctgcctgcat tggttaaatc
    14521 tgtcgagccg gacagcatcg catcggtgcc ttcgagggct gcgttgttga ggaacttctg
    14581 tgcgcccgcg agcgagacct cgttcagctt gagcttgccg tcaaacggag tgctcaacat
    14641 ctgcgcctga ttgatggggc cgcccttgcc actcagtgaa agcgtctccg cgcccttgcc
    14701 cggaacgtgc gccgccagat cgacggagaa cggctggttg ggagcgtagt cttttagcgt
    14761 cagatcgatg tggtcgtata ccgcgcgcgg ctgctttttc tggaagtcgg tgaccgcgac
    14821 ctgcccgtca gagatcgcga gtttatccag agcaaacgaa ccaccgtcac tcgcggccgg
    14881 agttggcgta gcggcaggtg gtttcgcctg agccggcggt tgagttggag cagcagcgtt
    14941 gttcgtgact ggtgccggga cctcaccgag gctggcgaaa ttccacactc ccgcctggtc
    15001 cttgatcaac tcgattttgg gacgcttcag cgtcaggctt tcgatcgcaa tcgtcttgct
    15061 gagcagcgga aacagcttga cgctgatgtc caattcgccg acatccgcaa acggaacggt
    15121 gctgtggtag cggggatctt ctccgatgga aacgttctgc acctgtaccc gcagcggaaa
    15181 gactgccaag tccatggccc ccagcgtgac cgggcggttg agacggctct gaaaccgggc
    15241 ctggatctcg ccacgatatt ggttgacgtc gatcagatgg ggcaacagca gcatcccgac
    15301 aacgacgagg acaatgacgg cgacaacacc gatcagaatt ttccgcatac gggcctccca
    15361 atttcatact gctggctgac gctgccccaa gtttatggaa atccggcctc tttagataga
    15421 ggagtctcat ggcagcgcag gtgcaaattc gcggggggac ggcgaatggt ttcttgttct
    15481 acaacgtcga atctttgaca acgatctatg gattgtatcg cgcgaagcgc cgttacgacg
    15541 cagtgttcga cgtctcgtct tcttcggcgt ggatcagccc aaacttcttc agcttgtaac
    15601 gcagcgcgtc gcggctgatg tcgagcaggc gggcggcgtg ggtctggttg ccgttggctc
    15661 gcgtcatggc catctcgacc agcgcgcgtt ccacttcttc cagcgacgta ccaccctcgg
    15721 gaatggagag tctgggcagg ctgcggccat ccggtaactg ggtgacacct ccgccgttgc
    15781 cactgctgtt gcccgatgac atctcgaatg cggtgtagga agcctgctgc tgcgccaccg
    15841 cgaagggcag gtagtcgggt cgcagaacgt tgccttcttc gaggatcatg gcccgctcga
    15901 ccgcgttctt cagttcgcgc acgtttcccg gccagtcgta cttgagcatc agccgccgcg
    15961 attcatcggt gatgccctgc accgttttac ggaacttgcg gttgtagcgg tcgatgaagt
    16021 actcgaccag cggcagaatg tcttctttgc gttcccgcag cggcggcaga aaaatggaaa
    16081 taatcgccag ccgatagtac aggtcctggc ggaacgtgtt ctcccgtact ccacgctcca
    16141 ggtcgcgatt cgatgcggcg atgacccgca catccacctg catgtcgcgc actccgccca
    16201 cgcgccggat gacctggtct tcgagaacgc gcagcaactt ggcctgcaac agaggcgaca
    16261 gttcgccgat ttcatccagg aagagcgtgc cgccgtcggc catttcaaac atgcccttct
    16321 tcatcgcctt ggcgtcggtg aacgcaccgc gctcatggcc gaagagttca gcctccatca
    16381 aggtttcagg aatcgccgag cagttgatgg caacgaaggg cttgtcctgg cggcggctct
    16441 ggtagtgaat cgctttggcg atcaggtctt tgccggtacc gctttcgccc tggatgagga
    16501 cggttgtggc ctcgctggcg gcgaccttat tgacgaaccc cagcaactcc gccatcttct
    16561 ccgacacgcc aatcacattg tgatacccgg cgcggcggcg aacttcaccg cgtagcgact
    16621 cgacctcggt gcgcagacgg gtggtctcca gtgcgttctt gacggcgacc gccagctcgt
    16681 cgaaatcgag cggcttgccg acaaagtcgt aggcgcccag cttgagcgcg gtcttaacgt
    16741 cttccaactg cgggtcggcg gtgatcatga tgatcgcccg cgcgtccccg gctttcttga
    16801 tttgttcgag gatggcgatg ccgctggcat cgggcaggcg cacgtctaga agcacgaggt
    16861 cgggagactc gttatgggcc acgcgaagcg cagccgcgcc gttgtcagcc tccagtacgt
    16921 ggtatcccca ctcttcgcac ttctgcctga gagaccaacg aaccagcttc tcgtcatcta
    16981 cgatgagaac c
//
LOCUS       DASRKK010000186         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_110439, whole genome shotgun sequence.
ACCESSION   DASRKK010000186 DASRKK010000000
VERSION     DASRKK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110439"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFC15_04135"
     CDS             complement(<1..944)
                     /locus_tag="VFC15_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZM09380.1"
                     /translation="MKIASCILLAIVLSIPWWCAAAPVSPAVSAANSKTATVTVAGDK
                     TWVDTGMDVNAGDKLHITATGTVNMGNNTGVTPNGVQRGWVDTLRALTLPSAGRGALV
                     GRIGNSDAATPFLIGADGTVLAPVAGLLYLGINQDSTQTPDGRYEVQIDRIAASAATA
                     SGAAAGKNNYDFQPVFATLDKKLPYRVTDKAGEGGNPGDLVNFVLVGSQEQVTSALKA
                     AGWLQADKTNTDAVVSAVLATLQKNPYMTVPMSILYLFGRPQDFGYERTEPVMVAAQR
                     NHFRIWNAPWSTPQQTIWAGAGTHDVGIERDQRSANAIT"
     gene            complement(1023..1211)
                     /locus_tag="VFC15_04140"
     CDS             complement(1023..1211)
                     /locus_tag="VFC15_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09381.1"
                     /translation="MLRKPDGTAQVYGKVAVDMRTGDIWGFPTTVQGPYPVDNAKTVP
                     PKSHPMYLGKFMFSEATR"
     gene            complement(1536..2630)
                     /locus_tag="VFC15_04145"
     CDS             complement(1536..2630)
                     /locus_tag="VFC15_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09382.1"
                     /translation="MATSTTPVTMRGSVLGVILHDVCEEIRLEQLRDILGARRVEPGF
                     KHATPDYVRFENPPVVERLEPVVLTSGEKLVPQIKYYDYGVVSVLFELGFEADWETLV
                     QLAARWVPNPELERQAQQIIRDRLDRIAPALVRPYREWLSEDYYIFTLSSVPGQPRGS
                     DLLTQHGPQIAQILRGELTPLARGENDDVLQASLSYYPNDLVVVGWNAALVYDTPAGA
                     ETSVQILEYVNSQLLEFRHYDELLTRELAGVYTSLKHRAGFWRRWRMRREASRLNSVT
                     VEVTELVERADNAIKFVSDMFSARLYRLAAAKVGVPEYKNLVNEKLRTAEGLYRFLVD
                     EFHQSRGFILELMVVIILVIELVYLFRGKY"
BASE COUNT          524 a          792 c          806 g          542 t
ORIGIN      
        1 gtgatcgcat tggccgagcg ctggtcgcgc tcgatgccga cgtcgtgcgt tcccgcaccg
       61 gcccagatgg tctgctgcgg cgtgctccag ggcgcattcc agatgcggaa gtggttgcgc
      121 tgcgccgcca ccatcaccgg ctcggtacgt tcgtaaccga aatcctgtgg acggccgaag
      181 agatacagaa tgctcatcgg cactgtcatg tagggattct tctgcagggt ggccaacacc
      241 gcgctgacga ccgcgtccgt gttggtcttg tcggcttgga gccagccggc agccttcaat
      301 gcactggtca cttgctcctg tgagccgacg agcacgaagt tgaccaggtc gccaggattg
      361 cctccctcgc ccgccttgtc ggtgacgcgg tacggcagct tcttgtcgag cgtggcgaac
      421 acaggctgga agtcgtagtt gttcttgccg gcagcagcgc cgctggctgt cgccgcgctg
      481 gccgcgatgc ggtcaatctg tacttcgtac ctgccgtccg gagtttgggt cgagtcctgg
      541 ttgataccca gatacagcag gccggctacg ggcgccagca cggtgccatc ggcgccgatc
      601 aggaagggcg tggcggcatc gctgtttccg atgcgcccga ccagagcgcc gcgtcccgcg
      661 ctcggtagcg tcagcgcgcg caaggtatcc acccatccgc gctgcacacc gttgggcgtc
      721 acgccggtat tgtttcccat gttgacggtg ccggttgccg tgatgtggag cttgtctccg
      781 gcgttaacgt ccatgccagt gtcaacccag gtcttatcgc cggcaacggt aacggtcgcg
      841 gtcttgctgt tggccgcact gacggcggga gacaccggag cagcagcgca ccaccagggg
      901 atgctgagaa cgatggcgag aagaatacag cttgcaatct tcatcgaacc aaggattcgc
      961 tcctcaccaa attataaaca gcgaggcgcc gaaggcgtgc agggtggttg ccgcgatagc
     1021 gcctaccgcg tagcttcgct gaacatgaat ttccccagat acatgggatg tgacttgggc
     1081 ggcacggtct tggcgttgtc aaccgggtac gggccctgca ccgtggtcgg gaatccccag
     1141 atgtctccgg tgcgcatgtc aacggcaact ttgccataca cttgcgctgt cccgtcgggc
     1201 ttacgcagca tggtgtaacc cggttcgagg tgaaagggtt aggcctcact ggcttgtgcc
     1261 tgcactggcg ccgggtggac gacgggacga aaggcggcca tcccaagtag aactgcgata
     1321 actacgagca ttgttttcgt gaagcggtcg agcgtcatag gtcctcccct aattaaggtc
     1381 cgcagaatag agtgacattc ccatcaggtt tgtcatcacg agcgaagtga gggacctgct
     1441 ggggcatggt catggaaaaa gcagattcct cgcaccaaaa tctggcgctc ctaatgacaa
     1501 ttcgtaagtc cagggtcacg ccttccgccc tgactctagt attttccgcg gaagagataa
     1561 accagttcaa tgactagaat gatcaccacc atcaattcca ggatgaagcc gcgactctgg
     1621 tgaaattcat cgaccagaaa gcgatacagt ccttcggcgg tccgcagctt ctcattgaca
     1681 aggttcttgt actccgggac tccgaccttg gcggctgcca gcctgtaaag gcgcgccgaa
     1741 aacatgtcgc tgacaaactt gatggcgttg tcggcgcgct ccaccaactc ggtcacttcg
     1801 acagtcacgg agttcagccg ggaggcctca cggcgcatac gccagcgccg ccagaagccg
     1861 gcccgatgtt tcaacgatgt atagacacca gccagctcac gggtcagcaa ctcgtcataa
     1921 tgacggaact ccagcaactg cgagttgacg tattccagga tctgcaccga agtttcggcg
     1981 cccgcagggg tgtcataaac cagcgcggca ttccatccca ccacaaccaa gtcgttggga
     2041 tagtaggaga ggctggcctg gagaacgtca tcgttttccc cgcgcgcgag tggagtgagc
     2101 tcgccgcgca gtatctgcgc aatctgcgga ccgtgttggg ttagaaggtc ggagccgcgc
     2161 ggctggccgg ggacgctgct cagtgtgaag atgtaataat cttcgctcag ccattcgcgg
     2221 tagggacgca ccagggcggg tgcgatacga tcgagccggt cgcgaatgat ttgctgcgcc
     2281 tgacgctcaa gctcagggtt cgggacccaa cgagccgcca gttgtaccag cgtttcccag
     2341 tcggcctcga atcccaattc aaacagcacg ctgacaacgc catagtcgta atacttgatt
     2401 tgcggaacca gcttctcgcc gctcgtcagt accacgggtt cgagccgctc cactaccggt
     2461 gggttctcga atcgcacata gtccggagtg gcgtgtttga agccgggctc aacccgccgg
     2521 gcgcccagga tgtcgcgcaa ttgctcgagc ctgatctcct cgcagacgtc gtggaggatg
     2581 actcccagga cggaacctct catggtcacg ggtgtcgtgc tggttgccat ggatcacctt
     2641 gctctcaagc cgtagcacaa gatt
//
LOCUS       DASRKK010000187         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_697097, whole genome shotgun sequence.
ACCESSION   DASRKK010000187 DASRKK010000000
VERSION     DASRKK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697097"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFC15_04150"
     CDS             complement(<1..390)
                     /locus_tag="VFC15_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09383.1"
                     /translation="MMINALKSRSIFFALLAVLALQPLTVTAQQTSRPAETGFSFDVY
                     GDSRSMMYLPYKADQEAEARELMVDMFELVLPEKVAAAAVKKSVKLTYDPSTNELVQM
                     VMPFITSSQVTTLTFEKGWVTEASVEDV"
     gene            710..1708
                     /locus_tag="VFC15_04155"
     CDS             710..1708
                     /locus_tag="VFC15_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZM09384.1"
                     /translation="MDFEIRLLRCALALAEHRNFARAASSLRLSQPSLSRSIQNLEKL
                     AGVQIFERGSRRVEVSDAGEIFLEYAQEVMSRSSDLSREMELLKGLAKGELQVGAGTY
                     VSVMFVDRVIARIVREHPEVRLRVANDNWANLLPLLRRRELDLAVVDVRAITDDQDFL
                     ITRLTRRQGYLAVRRGHPLLEQKRALSMSEVLHYPFVSTSRFPSAMLREFISESATGE
                     NPSRPGPKTVPSIACESLVMMKNIVQESDAVALLPLNLLLPDLDAKAIAVLPVVLPVL
                     QGEFGIVRLARRSLSPLGDLFVRTLLQVDAEVAAVEERAARKLFPAERRRARSAHA"
     gene            complement(2215..>2634)
                     /locus_tag="VFC15_04160"
     CDS             complement(2215..>2634)
                     /locus_tag="VFC15_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09385.1"
                     /translation="LHTRLLCLTTFFGVLLHSCCAQDLAPRAYIITPLHSNAITLSWA
                     FYNGSIDYNGGLPISEATGTYSVPTFSYYHSLSFFGRSANVVASLPYGVGNFQGTLGV
                     GQHLYRSGLADSVFRFSVNLKGGPATKRRRWPSGQRS"
BASE COUNT          542 a          759 c          779 g          554 t
ORIGIN      
        1 cacgtcctcg accgaggcct ccgtaaccca gcccttttca aacgtcaacg ttgtcacctg
       61 actggaggtg atgaacggca tgaccatctg caccaactcg ttggtggatg ggtcgtaagt
      121 cagctttaca ctcttcttaa ccgccgccgc cgctaccttc tccggaagca cgagttcaaa
      181 catatcgacc atgagctcgc gagcttccgc ttcctggtcg gctttgtacg gcaggtacat
      241 catcgatcgc gagtcgccgt acacgtcaaa cgaaaacccg gtctcggcag ggcgcgacgt
      301 ctgctgcgcg gtcaccgtca gcggctgaag ggccagcacg gcaagtagtg cgaagaagat
      361 ggatcgagat tttagggcgt taatcatcat ggtttcctca cgtgctggat gtttctttgt
      421 cgagctgatt tcttaggccg gacgcagtgg ttttccacgc actgagcgac gcacgtcgat
      481 catgagcggg cagccagccg cttttgtccg actgtttccg ggttgttggc gaaacaaagt
      541 ggtcatgacg gacttcagat tgaagtctcc agactgcggc gacaccctgg gcgcttgggg
      601 cgagggcaag tcgcgcggcg taagctactg tatctgtaga ggaaagtcta gtgaaggtag
      661 cccctatgcg tccaatgttt ttctaacatc aacctgatag cctagacgta tggacttcga
      721 aatccgcctc ctgcgttgcg cgctggcgtt agcggaacac cgcaatttcg cccgcgccgc
      781 cagttctctc cgtctttcgc agccgtcgct gtcgcgcagc atccagaacc tggagaagct
      841 ggccggggta cagatcttcg agcgcggctc gcgccgggtg gaagtcagcg atgcgggcga
      901 gatttttctg gaatacgcgc aagaggtgat gtcgcgctcc tccgacctca gccgggaaat
      961 ggagttgctg aagggcctag ccaaggggga actgcaggtc ggagccggaa cctatgtctc
     1021 ggtcatgttt gtcgaccggg taattgcgcg gattgttcgt gagcatcccg aggtgcggct
     1081 gcgcgtggcg aacgataatt gggcaaactt gctgcccctg ctgcgccgtc gcgaactcga
     1141 cctggcggtg gtcgatgtgc gcgccatcac cgacgaccag gatttcctca tcacgcggtt
     1201 gacccggcga caaggctacc tggcggtgcg gcgtggacat ccgctgctcg agcagaagcg
     1261 cgcgctcagc atgagcgagg tgctgcacta tccgtttgtg agcacgtcgc ggtttccgtc
     1321 ggccatgttg cgggagttca tcagcgagtc ggccacgggc gagaacccct cacgccccgg
     1381 accgaagacc gttccctcga ttgcctgcga gtcgttagtc atgatgaaga acatcgtcca
     1441 ggaatcggat gccgtggcct tgctgccgct gaacctgctg ctccccgacc tcgacgccaa
     1501 agcaatcgcg gtattgccgg tggtgctgcc cgtgctgcaa ggtgaatttg gcattgtgcg
     1561 actcgcgcgg cggagcctgt ctccgctggg ggatctcttc gtgcgcacgc tgctccaggt
     1621 agacgccgaa gtggctgccg tggaagagcg ggccgccaga aaattgttcc ccgccgaacg
     1681 acgccgtgcc cgttccgcgc acgcctagat ctcttcatgg cggacattca ggaggcgaac
     1741 aactctagca acaagtaatg gtccggttgg cagcgacaaa atgcccccaa caaagggaag
     1801 cgttgccgcc gactcgtcaa gtgaagccgc gatacgcaaa cagcagacct tcattcaccc
     1861 gccgtttgag atcacacaga atggcgactt tggtcacggt atgacatgcg ccaagtgcgg
     1921 gcataatagt ctccagacgg cgtgtacgat ttgtcgcgcc atgagagtga cataatcgga
     1981 aagtgacact tcacaccaga ttgttgtgct tgacgacttt ttttggagtg ttgctacatt
     2041 cctgctgcgc gcaggatctc gcgccgcgcg cctatatcat tactccgctg cattccaacg
     2101 cgatcacgct gtcctgggct ttctacaacg gcagcattga ctacaacggc ggccttccca
     2161 ttagtgaagc cacggggacc tacagcgtac cgacattcag ctattaccac tcgctcagct
     2221 tctttggccg ctcggccaac gtcgtcgctt cgttgccggg ccacccttca ggtttacgga
     2281 gaagcggaag actgaatccg ccagaccgga acggtaaagg tgttgcccca cgcccaatgt
     2341 tccctggaaa tttcctactc cgtaaggcaa cgaagcgacg acgttggccg agcggccaaa
     2401 gaagctgagc gagtggtaat agctgaatgt cggtacgctg taggtccccg tggcttcact
     2461 aatgggaagg ccgccgttgt agtcaatgct gccattgtag aaagcccagg acagcgtgat
     2521 cgcgttggaa tgcagcggag taatgatata ggcgcgcggc gcgagatcct gcgcgcagca
     2581 ggaatgtagc aacactccaa aaaaagtcgt caagcacaac aatctggtgt gaag
//
LOCUS       DASRKK010000188         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_501783, whole genome shotgun sequence.
ACCESSION   DASRKK010000188 DASRKK010000000
VERSION     DASRKK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501783"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1207
                     /locus_tag="VFC15_04165"
     CDS             221..1207
                     /locus_tag="VFC15_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM09386.1"
                     /translation="MTDRLPNDVSVRRLHGILLVLLMVVLAGSAGASTFTYSTGTNLT
                     LGTAQQVSLTPDLVMGQVAPNFSLATAQPVSPQYMSYEILGTINATHPQEYFGFGANV
                     NDNINMLVRPINPATQPTELLLYDNLANLVAIASGNYSDGVSSTIQYTVFSGGSGTWT
                     SEVTSPSSGSFKYDLSFASPFGAPYTIDVLGSRNQPTHDGFYSISANAGDNLHLVVNQ
                     TGTQSVATELLLYDENGNLVAIASGNASNGLSSIIDFSVPSGETGNWTAEVSSFNPAT
                     LYNYDLSILGASGFGPINPLSPPPVVPEPSSLLLMGTGLLAAIGAVRRRLRG"
     gene            1372..1896
                     /locus_tag="VFC15_04170"
     CDS             1372..1896
                     /locus_tag="VFC15_04170"
                     /inference="COORDINATES: protein motif:HMM:NF045136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZM09387.1"
                     /translation="MIRSYKGIWPAIPDSCYVDESAQVLGDVVLGENASIWMNAVVRG
                     DVNYIRIGANSNIQDCSVMHGMLNQWPVVVGDWVTVGHNVTLHGCVVEDRCLIGMGVI
                     ILNGARIGAGSIIAAGTLIPEETVIPPGSLVMGIPGKVRKQLGDDDQETILRYAKNYL
                     GYKETYLAERKKSG"
     gene            1967..3250
                     /gene="hisS"
                     /locus_tag="VFC15_04175"
     CDS             1967..3250
                     /gene="hisS"
                     /locus_tag="VFC15_04175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZM09388.1"
                     /translation="MIRAIRGTRDLLPPDTDLWNRVDAKVRDVFARYNFHEIRTPIFE
                     DTSLFTRSVGEDTDIVTKEMFTWEDKGRAASEKSQSVTLRPEATAGTVRAYIEHGLDK
                     KGTLQKLYCIGPMFRRERPQKGRYRQFYQINAEIIGPPSAGSESPARDAEVLEMVATL
                     LDELGLRGWTLQLNSVGCAADRARYNQALREALQPVVATMCVDCQRRAQTNPLRVLDC
                     KVPADQPIIEKLPKISDYLDEPCRENFAAVRAMLDAVGVPYSINERMVRGLDYYTRTT
                     FEFTHGDLGAQSAVLGGGRYDGLSESLDGPKAPGIGFAIGEDRLILALQAEGVTADAK
                     LDAYIAPLGAGMNVHALILARELRRAGVSADIGDETFRLKKSFESAEKAGAKYIVIVG
                     ENEIQSGTFAVKELSTGKQAQVPRADLTNYLKREG"
     gene            complement(3345..3782)
                     /locus_tag="VFC15_04180"
     CDS             complement(3345..3782)
                     /locus_tag="VFC15_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09389.1"
                     /translation="MPHTLSRNLVHCVFSTKGRLPLISEPEMLWTHLGVIAKSKDIAL
                     ITAGGTRNHVHLLLALSPVMTLAKTIQDLKAHTSRWMKKDVAAFTWQEGYGAFGVSES
                     CRQTVVDYIAHQAEHHQKWTYEQEFLTLVRKSGVEYDPQYLFG"
BASE COUNT          869 a         1226 c         1161 g          796 t
ORIGIN      
        1 ccaccggtcc ttgggaccaa attaccgcct cgagcggtcc atgtcacgaa catgtttaca
       61 catgttcgta tttcggtcgg tcgacggagc ccgtaggcca accaaagcat gactcgtggg
      121 tctgaggcga agcctcgtta gaattaggtc gcgcaattca gccgcttcgt ggagcgaacc
      181 gacctttaac tcacaggcaa aatcgaggag gaaggaaaac atgacggacc gtttaccaaa
      241 tgatgtttcc gtgcggcggc tgcacggcat tctgctggta ctgctgatgg tggtgctagc
      301 tggttcggca ggcgcttcca cgtttaccta ttcgacaggg acgaacctca ccctcggaac
      361 ggcacagcaa gtcagcttga caccagactt ggtcatggga caggttgcgc caaacttcag
      421 cctggcgacg gcccagcccg tcagcccgca atacatgtct tacgagattt tgggcaccat
      481 caatgcgact catccgcagg agtactttgg cttcggtgcc aatgtcaacg acaacatcaa
      541 tatgctggtg cgcccgataa accccgccac ccagccaacg gaactgctgc tgtatgacaa
      601 tttagcgaac cttgtcgcca tagccagtgg gaattattcc gatggtgtgt cgtcgaccat
      661 ccaatacacg gtgttttcgg gcgggagcgg gacatggacc tcggaggtaa cgagtccctc
      721 ctcgggatcg ttcaagtacg acctgagctt tgcctcgccg tttggcgcgc cctacactat
      781 cgacgtcctg gggtcgcgga accagccgac acacgatggg ttctactcca ttagcgccaa
      841 tgcgggagat aatctgcacc tggttgtcaa tcagaccggg actcagagcg tggctaccga
      901 gttgctgctg tacgacgaga acggcaacct cgtggccatc gcgagcggaa acgcgtcgaa
      961 cggcctgtcg tccatcatcg acttctcagt cccgagcggc gagaccggaa attggaccgc
     1021 agaagtcagc agtttcaacc ctgcgaccct ctacaattac gacctatcga ttctgggagc
     1081 ttccgggttc ggccccatca acccgctgtc gccaccgccg gtggttcctg aaccctcgtc
     1141 gctgctactg atgggcacag gactgcttgc cgcgattggg gcagtacgac ggaggctgcg
     1201 cggctagtct actaacggat attgggcagg ggccgctcga ttagagccgc ttttccttta
     1261 cggtcctggt tttgctccaa cgggatgccg tgttattttc aaagactagc cgataggtcg
     1321 aggagtgttc catgttcctg aactgcggcg caatcgtccc taaataaacc catgatccgc
     1381 tcctacaaag gcatctggcc cgccattccc gactcctgct atgtggacga gtcagcgcag
     1441 gtgcttggcg acgtggtgct gggcgagaac gccagcatct ggatgaacgc cgtggtgcgc
     1501 ggcgacgtga actacatccg catcggcgct aacagcaaca tccaggactg ctcggtgatg
     1561 cacggcatgt tgaaccagtg gccggtggtg gtcggcgact gggtcaccgt cggccacaac
     1621 gtcacgctgc acggttgcgt ggtcgaagac cgctgcctca tcgggatggg cgtcatcatc
     1681 ctgaacggcg cgcgcatcgg cgcgggctcg atcatcgccg ccggcacgct gatccccgag
     1741 gagacagtga ttccgcccgg ctcgctggtg atgggcattc ccggcaaagt gcgcaagcaa
     1801 ctcggcgacg acgatcagga aacgatcctg cgctacgcga agaattattt gggctacaaa
     1861 gaaacgtact tggcggaaag gaagaaaagt ggctagtggt cagtggttag tggatagcga
     1921 agactggccg tcctaactaa ccactatcca ctaaccacta tccactatga ttcgagccat
     1981 ccgcggaact cgtgacctcc tcccgcccga caccgacctg tggaaccggg tggacgccaa
     2041 ggtgcgcgac gtcttcgcac gctacaactt ccacgaaatt cgcactccaa tcttcgaaga
     2101 caccagcctg ttcacccgca gtgttggcga agacaccgac atcgtcacca aggagatgtt
     2161 tacctgggag gataagggcc gcgcggcgag cgagaagtcg cagtcggtga ccctccgccc
     2221 tgaggccacc gcgggcacgg tacgggctta cattgagcac ggactcgaca aaaagggaac
     2281 gctgcagaag ctctactgca tcggcccgat gttccgccgc gagcgtccgc agaaaggacg
     2341 ctatcgccag ttctaccaga ttaacgcgga gatcattggc ccacccagcg cgggcagcga
     2401 gtcgccggcg cgcgatgccg aagtgctaga gatggtggcc acgctgctgg atgagctcgg
     2461 cctgcgcggc tggacgctgc aactgaattc cgtaggctgc gccgccgatc gcgctcgcta
     2521 caaccaggcc ctgcgtgaag cattgcaacc ggtcgtcgcg accatgtgcg ttgattgcca
     2581 gcgccgtgcc cagaccaatc cgctgcgagt gctcgactgc aaggttcccg cagaccagcc
     2641 catcatcgag aagctgccca agatcagcga ctatctcgac gagccctgcc gcgagaattt
     2701 cgccgcggtg cgcgccatgc tcgacgccgt cggagtgccg tactcgatca acgagcgcat
     2761 ggtgcgcggc ctcgactact acacccgcac cacattcgag ttcacccacg gcgatctggg
     2821 cgcgcagagc gcggtgctcg gcggtggacg ttacgacggc ctgtccgaat cgctcgacgg
     2881 gcccaaagct cccggcatcg gattcgctat cggcgaggat cgcctgattc tcgctctgca
     2941 ggccgagggc gtgactgccg atgccaaact cgacgcatac atcgctccac tgggcgcggg
     3001 aatgaacgtg cacgcactga tactggcgcg cgagttgcgc cgcgcaggcg tgagcgccga
     3061 catcggcgac gaaaccttcc gtctgaagaa gtccttcgag tccgccgaaa aagctggcgc
     3121 gaagtacatc gtcatcgtcg gtgaaaacga gattcaatcc ggcacattcg ccgtaaaaga
     3181 actctccacc ggcaaacaag cacaagtccc gcgcgctgac ttgacgaact acctgaagcg
     3241 cgaaggctag tccttgagcc cgtagggcgt gcatgacagc cgagcacgca gcgaagcgga
     3301 gtgttgggta gggtccgtta gagaacccga gtgccccggg cacgtcaccc aaacaaatat
     3361 tgcggatcgt attccactcc cgatttgcgc accaacgtga gaaactcctg ctcatacgtc
     3421 cacttctggt gatgttcggc ttgatgagcg atgtaatcca cgaccgtctg ccggcacgac
     3481 tcgctcaccc cgaatgcgcc gtatccctcc tgccatgtga acgccgccac atcttttttc
     3541 atccagcgag aggtgtgtgc cttcaaatcc tggatggtct tcgccagtgt catcaccgga
     3601 gagagtgcca gcaaaagatg cacgtgattt cgcgtcccgc ctgcggtaat cagagcgatg
     3661 tcctttgatt tagcgatgac acccaagtgt gtccacaaca tttcaggttc ggaaatcaag
     3721 ggaagtcggc ctttcgtgct gaagacgcaa tgcacaaggt tgcgcgatag ggtgtgaggc
     3781 ataggtacaa gctagtctgc ggcctcgtca catacggtga cggcgaacac attgcgggtg
     3841 tgacccggac atcgtccccc cgagggactc accaatcaat tgacgcacac ccagcactcc
     3901 gccttcgggt gcgtgctggg ctgtcatggc acgcccttcg ggctttcgga gacctgctgc
     3961 gcactcgaat gcaaaccaca tcaattagaa ttaatctttc cctctttaac taaaggactc
     4021 ttcatgctcg attttcttgg tgaattgaag cg
//
LOCUS       DASRKK010000189         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_208281, whole genome shotgun sequence.
ACCESSION   DASRKK010000189 DASRKK010000000
VERSION     DASRKK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208281"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFC15_04185"
     CDS             <1..463
                     /locus_tag="VFC15_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09390.1"
                     /translation="EQARLERLEQAPSTGPHAMVRMLASIIGVGVETADMLVQEILSR
                     NLRDRRAVARYAGLTGSPDESGVKRREKGLAKAGNARVRRGLIQLAWRFLRFQKESAL
                     AQWYRARTEGAAGKRRTTMIVALARKLLIALWRMVTTGEIPAGVALRTVAA"
     gene            complement(882..2023)
                     /locus_tag="VFC15_04190"
     CDS             complement(join(882..1759,1759..2023))
                     /locus_tag="VFC15_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM09391.1"
                     /translation="MARKRHTAEEIVAKLRQVDVLMAQGRQVADAVRAIGVTEVTYYR
                     WRNEYGGLKGDQVKRLKELETENNRLRRAVSDLTLDKLILAEAAKGKLLSPSRRRACV
                     DRVTAELGISERRACRALGQHRSTQRKTPTTPDDEAALTADIIELARQYGRYGYRRIT
                     ALLRKAGWTVNKKRVERIWRCEGLKVPAKQPKRGRLWLNDGSCIRLRPERPNHVWSYD
                     FVADRTHDGKAFRMLCIIDEFTRESLAIRVARKLKATDVIEALCELFVSRGIPAHIRS
                     DNGPEFVAEALRDWIAAVGAKTAYIEPGSPWENGYCESFNGKLRDELLNGEMFYTLKE
                     AQIVIENWRHHYNTVRPHSSLGYRPPAPEALVWPASKLVAQMQSLN"
     gene            2099..2590
                     /locus_tag="VFC15_04195"
     CDS             2099..2590
                     /locus_tag="VFC15_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09392.1"
                     /translation="MEFRPNATTNLLVSKTLVADNGSVGIFVVPTGSGTVTAVFNRVE
                     VTHNGNIDILVDGEVSTGTINATASDSVAARNNGGFYVLSAAGQAQTTFSLFHSVAAD
                     NLNGIVAQTTNAGLRIAQSMVTGNSGAGWLALSGGVIATYQDSYINGNGPNTGSLTPI
                     VRQ"
BASE COUNT          573 a          842 c          839 g          615 t
ORIGIN      
        1 cgagcaggct cggcttgaac gcctggaaca ggcgccgagc actggcccgc atgcaatggt
       61 gcggatgttg gcaagcatca tcggtgttgg tgtcgagaca gcggatatgc tggtgcagga
      121 gatcctgtcg cgaaaccttc gggatcgacg ggccgtagct cgttacgccg gcctcaccgg
      181 gtcgcccgac gagagcgggg tcaaacgacg tgagaagggc ttggcgaagg cgggcaatgc
      241 acgcgtgcgg cgcggcctca ttcagctggc gtggcgcttc ctgcgcttcc agaaggaaag
      301 cgcactggcg caatggtatc gagccaggac cgaaggtgcg gcgggaaagc gtaggacgac
      361 gatgatcgtt gcacttgccc gaaagcttct gatcgcactg tggcgcatgg ttacgaccgg
      421 agaaatcccc gctggtgtcg cgttgcggac ggtggcggct taaacatctt atcgaggagc
      481 agaaaatcca gtttcagcgt cctttgcgcc ttggcgacag ccgctgatca tgatccgagg
      541 tggtggtgac ccgaacacag ccatggcttt gaagccgtgt ccagaatggg cctgccgcct
      601 cggagcctca ccgcagaagc gaatagttgc ttcatggtcc gggtcccgca tggacccaac
      661 cgaatacaag gttgcggcgc gagcatcgcg cccgagcgtg ggctcccctc ggatcaacta
      721 taccgaacct cgaacaacga aggacataga gttgcagtaa cacttgacac gaaagtcctc
      781 atacaaggct gtgtgaaaac tccgatgtcc aacttgcgcg tagaacttct atctcgattt
      841 tgtcgattga ccggccccca cggagtggtc cgattggaat gttagtttaa cgattgcatt
      901 tgcgccacca gttttgatgc cggccagacg agtgcttctg gcgccggcgg tcggtatccg
      961 agcgaagagt gcgggcggac cgtgttgtag tgatgccgcc agttttcaat cacgatctgg
     1021 gcctccttca atgtgtagaa catctcgccg ttgagcagtt cgtcacgcag tttaccgttg
     1081 aagctttcgc aatagccgtt ctcccatggg cttccaggtt cgatgtaagc ggttttggct
     1141 ccgacggcag cgatccagtc ccggagagcc tctgcgacaa actcagggcc gttgtctgag
     1201 cgtatgtgcg caggaatgcc tcgagagacg aacagctcgc acagggcctc gatgacgtcg
     1261 gtggccttga gcttccgtgc cacacggata gccaggctct cacgggtgaa ctcgtcgata
     1321 atgcacagca tccggaaggc tttgccatcg tgggtacggt cagcgacgaa gtcatacgac
     1381 cagacatgat tagggcgctc cggccgcaga cggatgcagg agccatcgtt gagccagaga
     1441 cgcccgcgtt taggctgttt ggcaggcact ttcagcccct cacatcgcca gatccgctcg
     1501 acacgcttct tgtttaccgt ccagccagct ttgcgaagca gcgccgtgat ccggcgatat
     1561 ccgtagcggc catattggcg ggcaagctcg atgatgtcgg cagtcaacgc tgcctcgtca
     1621 tcgggtgtcg tcggagtctt gcgctgcgtc gatcgatgtt ggcccagtgc tcggcatgcc
     1681 cgtctctcgg agataccaag ctcagccgtc acacgatcca cgcaagcgcg gcggcgcgag
     1741 gggcttagaa gtttcccttt gcggcctctg caaggatcag cttgtccagc gtcagatccg
     1801 acaccgctcg ccggagccga ttgttctcgg tctccagctc cttgagccgc ttaacctgat
     1861 cgcccttgag gccgccatac tcattgcgcc accgataata cgttacttcc gtcacgccta
     1921 tcgctcgaac cgcatccgcc acctgccgac cctgcgccat cagcacatcg acctgacgca
     1981 gctttgcaac aatctcttcc gctgtatgcc tcttccttgc cattgatcgc atcctccatg
     2041 gtcaaaacca tacctcaggg aggaccactt caaagggggc agatcaaccg ttaacggcat
     2101 ggaatttcga cccaacgcca cgaccaatct gttggtctcg aaaacgttgg tggccgataa
     2161 tggcagtgtt gggattttcg tcgtaccgac tggatcgggc accgtcacgg ccgtcttcaa
     2221 tcgcgtcgaa gtgactcata atggcaatat cgatatctta gtggatggag aggtcagcac
     2281 tggcacgatc aacgccaccg cgtccgacag cgtggccgcc cgcaataacg ggggctttta
     2341 tgtcctatcg gccgcgggcc aggctcaaac cacgttctcg ctgtttcatt ccgtcgccgc
     2401 cgacaatttg aatggcattg ttgcgcagac gaccaacgcg ggtttgcgca tcgctcagtc
     2461 gatggtgaca ggaaattcag gcgccggttg gctagcgctt agtggcggcg tgatcgcgac
     2521 ctatcaggac agttacatca acggcaatgg gcccaatacg ggatcgttaa cgcctatcgt
     2581 caggcaatag cgccgaagcg ccgtaggcgg ttgccgacga ctgttcggac cattgttcct
     2641 attgtggaac ttcgcactcg gaagtcgagc agctagccag ctttcatatt ttcaagatac
     2701 atttatttcg cgcgcaattc gcgtgcttag gcaacccttt ggggggctgt catgaagatc
     2761 attgctgctt tcgctgtcgg gctggcactt gccgtcgcac ttccggccgt atccgcccag
     2821 gcgcagaacg cgcgctcttt tgtgtccggc cacggcctcg ataccaacg
//
LOCUS       DASRKK010000190         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1088324, whole genome shotgun sequence.
ACCESSION   DASRKK010000190 DASRKK010000000
VERSION     DASRKK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088324"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFC15_04200"
     CDS             <1..208
                     /locus_tag="VFC15_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09393.1"
                     /translation="DVAGHDLDAARVRLLWACAACTPHRQFLRGNASPTLNYRLWCSP
                     RVRGVRAGAFDVGQRARLAFRCAP"
     gene            558..1958
                     /locus_tag="VFC15_04205"
     CDS             558..1958
                     /locus_tag="VFC15_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09394.1"
                     /translation="MRLYVPRVDRTVVNHGLAPEQGRTGDPQDGAYFIFKSMEQGASF
                     RITVPKYATKDPNHRILARQKSRYTHYYFYIRDEVLGPMVMRVGSFFPFQTTYYLNGH
                     SFIEQELKRAHIGFRKNDNAFLGVDDVAALQAAADRLSPEIIRERLDYWTFVLGPKFS
                     DKERVAMNLSRFYAINQIEYCRNFIFKRHFPIHKLFERSCEIGLWRLTANRIAEIFGT
                     RLNRRMRGKLSTVIDQIEHGHHVFRAYFKNAFLKQYEKFSTFLRNELVSNNLSDFGLK
                     KSLDQLDAVRQKFQTITASFAGFQAQWLNVHADFPLLQRLAMPVTVGRVRYPGIKVHD
                     TRTIRLLEVLLHGSTKVGGWTAREIHQAVLTSFDLSDHAYGINQLRYDLRKLKGHGLI
                     ERDASRYAYRLTHQKGTETALLLLFFHKRLCGPLANSRFHHRPNPGHRPDSRLEAAYH
                     RADKAIDNIVQLLEAA"
BASE COUNT          488 a          757 c          631 g          470 t
ORIGIN      
        1 tgatgtggcc ggccacgacc tggatgcggc gcgcgttcgc ttgctttggg cctgcgccgc
       61 ctgcacacca catcgtcagt ttctgcgcgg caatgcatcg cctacgctca actaccggct
      121 gtggtgtagc ccacgggtgc gaggtgtcag ggcgggcgcg tttgacgtgg gtcagcgcgc
      181 ccgtctggcg ttcaggtgtg cgccataacc gacatcccga tcggactagc ggtcgttggg
      241 cgccagcata gatggtgctc tctccgcagt ctcgaacggg ctggcgatcg ggtccagtct
      301 accggcttga tcaacatgcc attcgtcgcg gggaacaagc gtggcgtcct tccacacttc
      361 gataccagtg ggaattactg ccatgaggat gcggtatcca cagcgcgcgg gtgctagcta
      421 gctccgcgca ttcgaaccgt tcttaacagc ccgccagtcc ggacacagaa caggctgggg
      481 ctgtcgcaag tttacggatt tgttcggcag tccggcggtt tcgtcagctc gaaagccagc
      541 ttgagcacgg gaccacggtg cgtctgtatg tgccccgcgt ggaccgcacg gtcgtcaacc
      601 atggcctggc tcccgaacag ggccgaacag gggatcccca agatggcgcc tatttcatct
      661 tcaagagcat ggagcaagga gcaagcttcc ggatcaccgt gccgaaatac gccacgaagg
      721 acccgaacca ccgcattctc gctcgccaga agagtcgcta tactcactac tacttctaca
      781 tacgcgacga ggtccttggt ccgatggtga tgcgggtggg ctcgttcttc ccgttccaga
      841 cgacctacta cctcaacggc cacagcttca ttgagcagga gctgaaacgc gcgcacatcg
      901 gtttccgcaa gaacgacaac gcgtttcttg gcgtcgacga tgtcgcggca ctccaggctg
      961 ccgccgaccg gttgagcccc gagatcatcc gcgagcggct cgattactgg accttcgtcc
     1021 tggggccgaa attctccgac aaggaacgcg tggcgatgaa cctgtcgcgc ttctatgcca
     1081 tcaaccagat cgagtattgc cgcaacttca tcttcaagcg ccatttcccc atccacaaac
     1141 tcttcgaacg cagctgcgag attggcctgt ggcggcttac cgcgaaccgg atcgcggaga
     1201 tcttcggcac gcgcctcaat cgcaggatga ggggcaagct ttctaccgtc atcgaccaga
     1261 tcgagcacgg ccaccacgtc ttccgggcct acttcaagaa cgccttcctg aagcagtacg
     1321 agaaattctc gacgtttctt cgcaatgagc tggtttcaaa caatcttagc gacttcggcc
     1381 tcaagaaaag cctggatcag ctcgatgccg tccgccagaa gttccagact atcactgcca
     1441 gcttcgccgg cttccaggcg caatggctca acgtccatgc cgattttcct ctcctccagc
     1501 gcctcgccat gcccgtgacc gtcggccgag ttcgctatcc agggatcaag gtccacgaca
     1561 cgcgcaccat ccgtctcctg gaggttctcc tgcatggcag tacaaaggtc ggaggctgga
     1621 ccgcccggga aatacaccaa gccgtcctca cctccttcga tctctccgac cacgcctacg
     1681 gcatcaacca gctccgctac gatttacgca agctcaaggg gcacggtctg atcgagcgcg
     1741 acgcctcccg ttatgcctat cgcctcaccc accaaaaggg caccgagacc gccttactcc
     1801 tcctgttctt ccataagcgg ctctgcggcc ctctcgccaa cagccgattc caccaccgcc
     1861 cgaacccggg ccaccgcccc gatagcaggc tcgaagccgc ctatcaccgc gccgacaagg
     1921 ccatcgacaa catcgtacaa ctccttgaag cagcataatt ttctcagagt gtcgactcgt
     1981 cttgtcaagg atttgatacg gtagaatcta gggaaatgac ggacaacata aataaataca
     2041 atggaaaaca ctgacaagca gagggttgct gcggttgcgg actagggacg gcgttgcccg
     2101 atgccgcgct gcccgcctgg attgtagcga tttccgatca tgcaaccgaa ttttgtcccg
     2161 tcgtctgatc gcctctggaa agcccgtaag aaggctttac atcgtccggt gtcatttggt
     2221 aaggtctgcc cgaacaagag gcttaacagg gcaatccccg atgaccgttt ttggctacac
     2281 ccgcgtatcg acaaccgagc aagcggccga tgaccgctcg accctggaca cctagcgccg
     2341 caagat
//
LOCUS       DASRKK010000191         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_892911, whole genome shotgun sequence.
ACCESSION   DASRKK010000191 DASRKK010000000
VERSION     DASRKK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892911"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFC15_04210"
     CDS             <1..197
                     /locus_tag="VFC15_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZM09395.1"
                     /translation="ALGLHNGQLDASWDCLRKVGNLSSASVLVVLEDVIKHRRPEPGA
                     MGLLAAMGPGFCSELLLLRW"
     gene            complement(470..1198)
                     /locus_tag="VFC15_04215"
     CDS             complement(470..1198)
                     /locus_tag="VFC15_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM09396.1"
                     /translation="MTQQDFSRRSTATELMDTDPVSFKEFDDYLRDLERINVLTLAYR
                     PTLLWLKRALADFPAERPISVLDVGSGGGGMLRQLRKWARGKKLKLDLTGVDVNPWST
                     RSAAESTPPEMSIRFETADVLARMDPLQTDFIVSSSFTHHLGDADLVRFIRWMDRYAA
                     HGWFINDLQRHILPYFFIKYATRFLPVNRMARHDGPVSVARAFAADDWRHLLAEAGIP
                     AERACIEWFFPFRYSVSCRKEIET"
     gene            complement(1482..>2112)
                     /locus_tag="VFC15_04220"
     CDS             complement(1482..>2112)
                     /locus_tag="VFC15_04220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09397.1"
                     /translation="SFGQDAAWKRTLISLLPSHESPVCVDLACGTGDIAFLLAGKYPR
                     GRIAGVDITEPMLDIARHRNTHPNVSFANQDMGSLDIVSESVDIVTGGYALRNAPDLG
                     IAIDEISRVLKPRGVAAFLDFSKPGGKIPQKMEYWGLKTWTGLWGTLLHRNHEVYSYI
                     AESLQRFPDRPHLRDIFRDKGFSVVESRLFFGGVTELLMVQKLRHGEAP"
BASE COUNT          461 a          603 c          617 g          431 t
ORIGIN      
        1 cagcactcgg gttgcacaat ggacagctgg atgcctcctg ggattgcctg cgtaaggtag
       61 gaaatctttc ttcggcttca gtgctggttg tgctcgaaga tgtaataaag caccgccgtc
      121 ctgagcctgg cgcgatgggt ctgcttgccg ccatggggcc cggtttttgt tccgagcttc
      181 tacttctgcg atggtaaagt gcgtggttgt tgaattcggc agatatcttc gtgatcggta
      241 gctgcccagc gaggattacg taggggttcc cgacaggacc aagccgccga ttgatcaagc
      301 cctgcactga gctcccttac cgccgctgcg attcacggtg gggcggacca aactgcctcg
      361 catcatgacc caaacgtgct gtcgggctgg ggaccgatct cctttggcag agcgtagtaa
      421 cggggatttc catccatgaa acccagatag ggaagcgcat gatccgcagc taagtttcga
      481 tctcttttct gcacgacacg ctgtagcgaa agggaaagaa ccattcgata cacgcccgct
      541 ctgccggaat gccggcttca gccaacaggt gccgccagtc atctgctgca aaggctcgcg
      601 cgaccgaaac gggcccatcg tgccgcgcca tgcggttcac gggcagaaac cgtgtggcat
      661 attttatgaa gaaataaggc aggatgtgac gctgcaggtc gttgatgaac cagccgtgcg
      721 cggcataacg gtccatccag cggataaaac gaaccagatc cgcgtctccg aggtgatggg
      781 tgaaagacga actgacgata aaatctgtct ggagcggatc cattcgagcg aggacgtcgg
      841 ctgtttcaaa acggatggac atctccggcg gcgtggactc tgccgccgat cttgtcgacc
      901 aggggttgac atcaactcct gtcagatcga gcttcaattt tttcccgcgc gcccatttcc
      961 tcaactgccg caacatgccg ccaccgccgc tgccgacgtc gagaaccgat atcggccgct
     1021 ctgctggaaa atccgctagg gcgcgcttta gccagagcag ggtcggacgg taagcgagcg
     1081 tcaaaacgtt gatacgctcg agatcgcgca gatagtcgtc gaattcctta aaggaaaccg
     1141 gatcggtatc catcagttcg gtcgccgtgc tgcgccggct gaaatcctgc tgggtcacga
     1201 ttcactaccc gctgcttcga acaacatgct ctcgaccggc ggcgcagttt gcgcgggacc
     1261 gcgcaagcag ttccttcggc aacccggcac tctcctccac ggcatccagg acagcgtgct
     1321 ccccgggaac cacccagagc aagaaatcct ctgcccctcc gccgttaagg tcattcatgc
     1381 aggcaaggaa gtccagctgc aatcgtggaa ggaaccagac ccgatcgcac catatcgaag
     1441 ccggggcaca gcggtggcga tacgattctg ataggcatcg gtcatggcgc ttcgccatgc
     1501 cgcagttttt ggaccatcag cagttcggtg acccccccaa aaaagagacg ggattcgacc
     1561 acagaaaatc ctttgtcccg gaaaatatcc cgcaagtgag ggcggtcggg aaagcgctgg
     1621 aggctttcgg ctatgtagct atagacttca tgattgcggt gcagaagggt gccccacagt
     1681 ccggtccatg tcttcaggcc ccagtattcc atcttctgtg ggatctttcc gcccggtttg
     1741 gagaaatcga gaaacgccgc cacgccgcgg ggttttagaa cgcggctgat ttcgtcaata
     1801 gctattccga ggtccggtgc attccgcagg gcgtagccac cggtgacgat gtccacggat
     1861 tcggaaacga tgtccagcga tcccatatcc tggttggcga aactgacatt cggatgagta
     1921 ttgcggtgac gggcgatatc cagcatcggc tcggtgatgt ccactccggc gatgcggcca
     1981 cgcgggtatt tgccggcaag cagaaacgcg atgtcgccgg tgccgcaggc gagatcaacg
     2041 caaacagggg actcgtgaga gggaagcaat gaaatcagag tgcgcttcca tgccgcgtcc
     2101 tgtccgaaag aa
//
LOCUS       DASRKK010000192         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_599639, whole genome shotgun sequence.
ACCESSION   DASRKK010000192 DASRKK010000000
VERSION     DASRKK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599639"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFC15_04225"
     CDS             <1..279
                     /locus_tag="VFC15_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09398.1"
                     /translation="GGHAKGPVADLVMDAGGNLYGTTGGDGTYRFGSVFKLTHGGGGW
                     TYTSLHDFTGGSDGLLPRSNLVFDGSGNLYSTAYGGVDGNGVVFQITP"
     gene            475..2751
                     /locus_tag="VFC15_04230"
     CDS             475..2751
                     /locus_tag="VFC15_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09399.1"
                     /translation="MAVRPASGRRWFLVLASIALIYSCLAGLRTVADYDTGWQLATGR
                     WVVQHHRVPSVDVLSYTVQGQPWIYPVGAGVIFYLVFLIGGYTLLSSIGAAACVGTVA
                     LLLRRGNAASASLAVVAVPLIALRTPPRADAFSVVLFAAFLSLLWENYQTGRARLWLL
                     PLLMLVWVNVHFGFVAGLALLLAYTGLELLETIRGEVRRHAAVQRLRRAFGWLLCTAV
                     VTLVNPWGWGIYRALLLQTRAAGHQEVSIAEWTSLPLSWVAFSRVLWLRDTRGTLYLL
                     LTIAVVAAVVALFRAQWGAAILLLAAVYAPLRHIRMGAVFSCVAVIVGGSILWKEMLR
                     LSSHVRRPSRMHSIAAIGVVVMLAALASARCFDLVTNRHYFGGDTDNATFAAGLSWWF
                     PQRAVEFIQRQNLPGQVFNGYNAGGYLAWALGPQRGDYIDGRDTLFGTERIERARHLL
                     ESSPDSAPWQQEADRYHINTIILSLARYDGLQFVQLKDFCNSRDWRPVYLDEVSAVFV
                     RRQPETEDLLLRSKVDCATAPLPAGPPPNDSAGAFNRWANAASILSTLDRNYEALAAT
                     DKAIAIFPGSAIVHLVRAAVLSPLGRFPEAEHEYLEAVSLHPDQFTWSALADFYRKQN
                     RNAEAIQALKKAVQFQASPELSLVQLGYYSLQLGQPKEALAAFDKAVQSAAPELTNAS
                     GRGSFRYNVATGRARAYEILGDVQQAIPYQEEAIRLAPDAPQPWLNLAQLYHLDGRSA
                     DEERAKAHAATLTENQRR"
     gene            complement(3147..3842)
                     /locus_tag="VFC15_04235"
     CDS             complement(3147..3842)
                     /locus_tag="VFC15_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09400.1"
                     /translation="MPQAVQNNNGKNNLAFSNVQAYTLAVITLAIGIAVGYFARGSAP
                     VSTAGENTQAASAPTGMGNAPMGSAQLPGIGSGQQQQAPSPETVAKAAEPLLAQLKTN
                     PGDFDTVVKLGNLYYDSQAYPQAIEYYEKAVGLQPENADVRTDLGTAYWYTGNADKAI
                     ASFEKALTIRPNHAATLFNLGIVKWQGKKDPKGAIVAWEKLLQTNPDYPQREQVQMLI
                     ERAKMHGASGNKG"
     gene            complement(4018..>4312)
                     /locus_tag="VFC15_04240"
     CDS             complement(4018..>4312)
                     /locus_tag="VFC15_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09401.1"
                     /translation="KLMAERVVPTIVALRQHLDELCRQELETLREEFGPFTEDQDQAL
                     TALTIHITQRIASSLARELKELPERNEQDVLTAAVGRLFHLEQPSRAAAGAKN"
BASE COUNT          790 a         1248 c         1263 g         1011 t
ORIGIN      
        1 ggcggacatg ccaaggggcc ggttgccgac ctcgtcatgg acgccggggg taacctttac
       61 ggcacgactg gcggtgatgg gacctatcgc ttcggctccg tgttcaagct gacacatggg
      121 ggcgggggtt ggacttatac atcgctgcac gactttaccg gtggctccga tgggctactt
      181 ccgcggagca acctggtatt cgatgggagc ggtaatctct acagcaccgc gtatggagga
      241 gttgacggca atggtgtggt ctttcagatt acgccctagc atggaagctt tccgatggtt
      301 cttgcttgtt cggccgttag cttcctcgag accgtttcca aggtagcgac gacgtcgcct
      361 cgagggtggt ctttcagcac cctgcacaaa agaatgccaa ggctcgacaa aaaacatggg
      421 aggaagtcta aacgcgggca actcgccagc ccgggagtga ttagcggaga ggtgttggcc
      481 gttcgtcctg catccggccg gcgctggttt cttgtcctcg cctctatcgc cctcatctac
      541 tcatgtctgg ctggtctgcg cacggtcgcg gactacgata ccggctggca gctggcaacc
      601 ggacgatggg tagtgcagca tcatcgggtc ccgtccgtcg atgtcctttc ctacaccgtg
      661 cagggacagc cctggatcta ccccgtcgga gcgggagtga tcttctacct tgtgttcctc
      721 attggaggct atacgctgct ttcatcgatt ggggcggcgg cctgcgtagg cacggtcgcg
      781 ttgctgctcc gccgaggaaa cgcggcaagc gccagcctcg ccgttgtggc cgtgccactt
      841 attgctctac gcaccccccc acgggccgac gcctttagcg ttgtgctctt cgcggcattt
      901 ctctcgctgc tttgggagaa ctatcagact ggaagagctc gtttgtggtt actgccgctg
      961 ctgatgctgg tgtgggtcaa cgttcacttc ggatttgtcg cgggtctcgc gctgctgctc
     1021 gcctacaccg gattggaatt attggagacg attcgtggtg aggtgcgccg ccacgccgcc
     1081 gtgcaaaggc tgcgtcgtgc ctttggttgg ctgttgtgca cggccgtggt aacgctggtg
     1141 aatccctggg gctggggaat ctaccgtgca ctgctgctgc aaacccgtgc cgccggacac
     1201 caggaagtct cgatagcaga gtggacaagt ctgcccctga gttgggtcgc cttcagtcgc
     1261 gtgctttggt tgcgagatac ccgcggcact ctgtatctcc tactgaccat cgcggtggtt
     1321 gctgccgtgg ttgctttgtt tcgtgcacaa tggggagcgg cgattctgtt gctcgcggca
     1381 gtgtacgcgc cgctgcgtca tatccggatg ggagctgttt tttcctgcgt ggcggtcatc
     1441 gttggcggat caatcttgtg gaaggaaatg ctccgcctaa gctctcatgt gcgaagaccg
     1501 tcacgaatgc attctatcgc cgccattggg gtggtggtca tgttggcggc gctcgcttcc
     1561 gcgcgctgct tcgacctggt cacgaaccgc cactattttg gcggcgacac cgacaacgcg
     1621 acctttgcgg caggtttaag ctggtggttc ccgcaacgtg cagtcgaatt tatccagcgc
     1681 cagaacctgc ctggccaagt cttcaatggc tacaacgcgg gcggctacct tgcctgggca
     1741 ctgggaccgc agcgcggtga ctacatcgac ggacgggata cgctatttgg aaccgaacgc
     1801 atagagcggg cccgtcacct gctggaaagc tcgccggatt ccgctccctg gcagcaggaa
     1861 gccgaccggt accacatcaa caccatcatt ctttcgctgg cgcgctacga tggcctgcaa
     1921 tttgtccagc tcaaggactt ctgtaatagc cgcgattggc gtccggttta cctggacgaa
     1981 gtctccgctg tatttgtgcg ccgccagccc gagacggaag acctgctcct ccgctcgaag
     2041 gtggattgcg caaccgcgcc tttgccagcc ggtccgccgc ccaacgatag cgccggagcc
     2101 tttaatcgat gggccaacgc ggccagtatt ctttccacgt tggatcgaaa ctacgaggcg
     2161 ctcgctgcca ccgataaagc cattgcgatt tttcccggca gcgccatcgt gcatctggta
     2221 cgtgccgctg tgctctctcc gttgggtcgt tttccggagg cggaacatga atatcttgag
     2281 gccgtctccc tccatcctga tcaattcacc tggtcagcac tggcggactt ctaccgaaaa
     2341 cagaaccgta atgcggaagc gattcaggcc ctgaagaaag cggttcaatt ccaggcaagc
     2401 cctgagctgt cgctggtaca gcttggctat tactcgctcc agttgggaca accaaaggag
     2461 gcgctggctg cgttcgacaa agcagttcaa agcgccgcgc ccgaattgac caacgccagc
     2521 ggaagaggct cgtttcgcta caacgtggcc acgggtcgcg ctcgggccta tgaaatcctt
     2581 ggcgatgtcc aacaagccat cccttatcaa gaagaggcga tccgacttgc accggatgct
     2641 ccccagcctt ggcttaacct ggctcagctc tatcacctcg atggccgctc tgccgatgag
     2701 gaacgtgcta aggcacacgc tgcaacgctc acagaaaacc aacgtcgctg acgccggacg
     2761 ttgtgctgga gacgaacgcc atccctaccg ggtaatttcg aagaattccg agcatgcgta
     2821 cggaatgttt gcccagcaca agggacacgc gccgatattc gattacgaat tcttgacatt
     2881 cctgagcctc aggtgtcact atccactcct cgtcgctcgg ataagtacga aagtgttcaa
     2941 gcgggtctga cttttctctg cgaccgactg gctgccagcc gcactgctct aaccgacccg
     3001 ttcgggctga ttagggtccg gctctgtcac acctctccaa aaagagcagt tgaacctgta
     3061 ttcaactcgc tcagtagcga gcggtgcagg tgttttatcg agactcttgg aactggccta
     3121 atgcagcggc ttttgagcga ctcgtattac cctttgtttc cgctagctcc gtgcatcttc
     3181 gcgcgctcaa tcaacatctg cacctgctcc ctctgcggat aatcgggatt ggtctgcaaa
     3241 agtttttccc aggctacgat tgcgcctttg ggatctttct tcccctgcca cttgacgatc
     3301 ccgaggttaa acagcgtagc cgcgtgattt ggccggatgg tcagtgcttt ttcgaagctc
     3361 gcgatggcct tgtcggcatt gccggtgtac cagtaagcgg tgccgaggtc agtgcggacg
     3421 tcggcattct caggttgaag gccgacagct ttttcgtagt actcgatcgc ttgcgggtaa
     3481 gcctggctgt cgtaatacag gtttcccagc tttaccaccg tgtcaaagtc gccagggttg
     3541 gtcttcagtt gagccagcag cggctcggct gccttcgcca ccgtttccgg cgatggggcc
     3601 tgctgctgtt gcccgcttcc gataccgggc aactgcgcag agcccattgg ggcgttgccc
     3661 attccggtgg gagcggaagc ggcttgcgtg ttttcgccgg cagttgaaac cggggccgat
     3721 ccgcgggcaa agtagcctac cgcaattccg atggccaagg tgatcaccgc cagcgtgtaa
     3781 gcctgcacgt tgctgaaggc caggttattc ttgccgttgt tgttttgtac agcttgaggc
     3841 atgtttcgtg gactccttgt agcggttagt ggcagttagc atttagcact tagcagttag
     3901 catttagtaa aagcgcctct tagtaaagaa cgacgtccgt gaaggccccc tggcattgga
     3961 agccgaaagg tccaaccaag tgccaaatgc caagtgccaa gtgccaagtg ctacctgcta
     4021 attcttcgcg ccggcggcag cccgtgaggg ctgttcaagg tgaaacaggc ggcccaccgc
     4081 tgcggtcagc acgtcctgtt cattcctctc cggaagttcc ttcaattcac gggcgaggga
     4141 actggcaatt cgttgcgtaa tatgtattgt gagggctgtg agtgcctggt cctggtcttc
     4201 cgtgaaaggt ccaaattcct cgcgaagcgt ttccaattcc tgccggcaaa gttcatccag
     4261 gtgttgacgc agggcgacga tggtcgggac cactcgctcg gccatcagtt tg
//
LOCUS       DASRKK010000193         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_893462, whole genome shotgun sequence.
ACCESSION   DASRKK010000193 DASRKK010000000
VERSION     DASRKK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893462"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFC15_04245"
     CDS             <1..180
                     /locus_tag="VFC15_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09402.1"
                     /translation="PWYTLLDFEVGQVSRQRLRLQRRQSIFPTRKGAQHRAQTGDTLR
                     GGESCTMRCARWLKQ"
     gene            233..774
                     /gene="rnpB"
                     /locus_tag="VFC15_04250"
     ncRNA           233..774
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFC15_04250"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(774..881)
                     /locus_tag="VFC15_04255"
     CDS             complement(774..881)
                     /locus_tag="VFC15_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HZM09403.1"
                     /translation="MALFSMKLENLQQLFLKELRDLYDAEKQINDALPR"
     gene            complement(1034..2161)
                     /locus_tag="VFC15_04260"
     CDS             complement(1034..2161)
                     /locus_tag="VFC15_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HZM09404.1"
                     /translation="MSVVLYHGSGAQDFSLETLNPSIGKFEKLRANVRDLLRVRGHQS
                     ALRLLSLFPFQLHEASNYFNDDFHVLHATVALDLYEEARQLAGSQTDRAAAAQLAETV
                     SEIGPYVRFVAVSLDPNRNVEATNDGRGLKRSEINRLVYKYIGVEGGYLGDFSYQKHH
                     DFYVDLDLDVNPFNYSGTTRDRFIKILSEATPDVQARILCGILGRYPENSAPIRTQSM
                     RDEIETWIRRLTATGAVAAPSLKITSQVVERALGDAEQLLASTGATSGVDRVHTALHG
                     YLLQVCSSANIAVPEDASLTQVFKLMRTGHSAFSDLGPRPEDIGRVLNSLAVVMDVFN
                     PIRNRASVAHPNAELLPEAEAMLVINAGRSILHYLDSKLSK"
     gene            complement(2211..>3323)
                     /locus_tag="VFC15_04265"
     CDS             complement(2211..>3323)
                     /locus_tag="VFC15_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HZM09405.1"
                     /translation="VQDGEVHIDIAVIAAPTADFFGNADGSHGRSACGSLGFALADSV
                     YADHVIVVTDNLVPFPCIPWQIQGNNVDYVVEVDSIGDPSMIVSGTTQITRSPDRLRI
                     SELVACFLRDSGIMRDGFSFQAGAGGIALAFVDYLAGMMKQANVKAGFVRGGSTKYLV
                     DLLNEGLIGYILDGQTFDLDAVRSIASNPRHVATSPFTSYNFHGKGNFASMIDAAVLG
                     ATEVDINFNANVVTHSDGRLLHGIGGWQNCLFAGCTILAVPSFRDRIPVIVDEVTTVT
                     GPGELIDVVATERGIAINPRRQDLLDAVKGKGLPIRPIEELQAEEEKICGGKPAKPRL
                     LDRPVAVVKWVDGTVLDTVWQVVDDNPYAPKVSGAE"
BASE COUNT          716 a          985 c          963 g          659 t
ORIGIN      
        1 ccttggtaca cgctactcga cttcgaagtc ggccaggtct ctcgtcagag gctgcgcctc
       61 cagaggcgtc aaagcatatt ccccacccga aagggcgccc agcacagggc gcagacgggc
      121 gacacgctgc gcggcggcga gagctgcacg atgaggtgcg cacgatggct gaagcaatga
      181 cagcaacacg ggccgcagcc gctactgcaa gcatctataa ttaatctgtt agctgctggg
      241 ccggacggtc gctgccggcc cgtggacact ggtcgtccgc ttcggcggcc cgccctcggc
      301 gggagaccag gttgtgcggg caggtagagg aaagtccgaa ctccacagag cggtgtgccg
      361 gataacgtcc gggcggcgac gttcaagcgt cgttgacgga aagtgccaca gaaaatatac
      421 cgccccgcct caaccggggt ccccggcgag cgccgatttt gcgcttgccg gggtggtcag
      481 ggcgaggtaa gggtgaaaag gtgcggtaag agcgcaccgc gccgtcagta atgacggtgg
      541 cagggaaaac cccacacgga gcaagaccaa ataggggagg agcatccgcc tcggcgggcg
      601 cacgtgccgg ctcggtgcgt cacaacttcc gggtaggtcg ctagagccgc ccagtaatgc
      661 gcggcccaga ggaatgaccg tcgtgccgct ctttgtcttc gcagcttcag gcccatgagt
      721 ccggcgagga gaaggagcgg cacacaaaat tcggcttaca ggcccggcag ctatcatctt
      781 ggcagagcgt cattaatctg tttctccgcg tcgtaaagat cgcggagttc cttgagaaaa
      841 agctgttgca ggttttcgag cttcatcgaa aatagcgcca ttggggcctc cagagttgtt
      901 ttggaggtgt gatggcgcgc aatgcagagg tgttgtctca ctggagaccg cttgcagtga
      961 atgtaaggtg cagaatttgt ttcgcaagga ccggatccag agccggaggt acttctccca
     1021 aaaaatcgga cccttacttt gacaacttcg aatcaaggta gtgcagaatc gacctccccg
     1081 cgtttataac cagcatcgcc tctgcctcgg gcagcagctc cgcattcggg tgcgcaacgc
     1141 tggcccggtt gcggatggga ttgaaaacgt ccattactac cgcgagcgaa ttcaaaacgc
     1201 gcccgatatc ctcagggcgt ggccctaaat cgctgaacgc gctgtgcccc gtccgcatca
     1261 gcttgaaaac ctgcgttaag ctcgcatctt ccgggactgc gatgttcgcg gaagagcaaa
     1321 cctgaagcaa gtaaccgtgc aacgcagtat gcactctgtc tacgccactg gtagccccag
     1381 ttgaagccag tagttgttcc gcgtcgccta gggcgcgctc aacgacttgg ctggtgattt
     1441 tcaacgaagg cgcagcaaca gcacccgtgg cagttagtcg tcgtatccat gtctcgattt
     1501 cgtcccgcat agactgggtc cgaatgggcg cggaattctc cggataacgg ccaaggatgc
     1561 cacacaagat gcgtgcttgc acgtccggcg tcgcttcgct caatatcttg atgaaccggt
     1621 cccgcgtcgt acccgagtag ttgaacgggt tgacgtccaa gtcaaggtca acatagaaat
     1681 catggtgctt ttggtaacta aagtcgccga gatagccgcc ttcgaccccg atgtacttgt
     1741 acaccagcct attgatctcc gaacgcttca agcctctccc gtcgttagtt gcctccacat
     1801 ttcggttcgg atcaaggctc acggcaacaa agcgcacata cggaccaatc tctgagactg
     1861 tttcagccag ttgggccgct gcggctctat cggtctgtga acctgccaat tggcgcgctt
     1921 cctcgtacag atcaagcgca acagttgcat ggagaacgtg gaaatcatca ttgaagtagt
     1981 tggacgcctc atgaagctgg aacggaaaca gggacagtag acgcagggcg ctttggtgac
     2041 cccgcacgcg aagcaggtcg cggacgttgg ctcgcagttt ttcgaatttc ccgattgagg
     2101 ggttcagcgt ttccagcgag aaatcctggg caccactgcc gtggtacagc actacgctca
     2161 cagccgcctc tcctctgata ggctgcacat attttaggcc agctgacctg ctactctgcc
     2221 ccactcactt tcggtgcgta gggattgtcg tccaccacct gccacacggt gtcgagcacg
     2281 gttccatcca cccacttcac cacggccacg ggccgatcaa gcagtcgagg cttggccggc
     2341 tttccgccac agatcttctc ctcctcggct tgcagctctt cgatgggacg aattggcagg
     2401 cctttgccct tcaccgcgtc gagtagatcc tgccgccgcg gattgatggc aatgccgcgt
     2461 tccgtcgcga cgacatcaat cagttcgccc gggccagtca ccgtggtcac ttcgtcgacg
     2521 atcaccggaa tgcggtcgcg gaacgacggg accgcaagaa tcgtgcatcc tgcgaacagg
     2581 caattctgcc agccgccaat tccgtgcagc agccgcccgt cagagtgagt caccacattc
     2641 gcattgaagt tgatgtcaac ctcggttgcg cccagcacgg cggcgtcaat catggacgcg
     2701 aagttcccct tgccgtggaa gttgtacgac gtgaacgggc tggtggccac atgacgcggg
     2761 ttgctggcga tcgaacgcac cgcatccaag tcgaacgtct gcccatcgag gatgtatccg
     2821 atgagacctt cattgagcag atcaaccagg tacttggttg aacctccgcg cacgaagccg
     2881 gccttgacgt tggcttgttt catcatgccg gccaggtaat ccacaaacgc cagcgcgatg
     2941 ccacccgcgc ccgcctggaa agagaaaccg tcgcgcatga ttccgctgtc gcgaagaaag
     3001 cacgccacca gctcggaaat tcgcagacga tcgggactgc gtgtgatctg cgtagtgccg
     3061 ctgacaatca tcgaaggatc gccgatggag tcaacctcaa ccacgtagtc aacattgttg
     3121 ccttggattt gccatggaat gcaggggaac ggcacgagat tgtcggtcac aacgatgaca
     3181 tgatcggcat agacggaatc ggcgagcgca aatcccagcg aaccgcatgc cgaccttccg
     3241 tgcgagccat cggcattgcc gaagaagtcc gccgtgggcg cggcaatcac cgcgatgtca
     3301 atgtggactt caccatcctg cac
//
LOCUS       DASRKK010000194         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_14133, whole genome shotgun sequence.
ACCESSION   DASRKK010000194 DASRKK010000000
VERSION     DASRKK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14133"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFC15_04270"
     CDS             complement(<1..462)
                     /locus_tag="VFC15_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZM09406.1"
                     /translation="MLIPSDKLREECGVVAIYGHPEASKLAYLSLYALQHRGQESAGI
                     AASNGEQLQLHKAMGLVSDIFTADVLALIPGSLAIGHTRYSTTGDSALLNAQPIMVEC
                     NKGKIALAHNGNLTNAAEVRSHLDQAGSIFQTTSDTEVVVHLIARSREQTLD"
     gene            complement(736..3078)
                     /gene="purL"
                     /locus_tag="VFC15_04275"
     CDS             complement(736..3078)
                     /gene="purL"
                     /locus_tag="VFC15_04275"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZM09407.1"
                     /translation="MYPVDSATSLAKEPTITPEALRDHGISAEEYDRILKSLGREPTF
                     TELGIFSVMWSEHCSYKSSRVHLKRLPTHSRRVVQGPGENAGIIDIGDGWACAFKIES
                     HNHPSFIEPFQGAATGVGGILRDIFTMGARPVAIMDSLRFGPITPQAGLDQTTLHKNH
                     SVLEGVVSGVASYGNCFGVPNIGGEVKFESCYSGNPLVNAFALGLVRRDQIFYAKAAG
                     EGNPVIYVGSKTGRDGIHGATMASEEFSEESEAKRPNVQVGDPFLEKLLLEACLEAMQ
                     TGAVVGIQDMGAAGLTCSTCEMGGRGGVGLEIELDLVPQRETGMTPYEIMLSESQERM
                     LLVAEKGREDEVFRVFQKWGLDAVTIGRVIDQPAMRVLEHGKVVAEIPNAALTDDAPL
                     YHRPLERWEPPVPREVPASIELGRKTEFTDDLKRLLASPNICSKRWVFQQYDSMVQIN
                     TVEGPGGEGGVIRIKGIRRGLAMALDGNSRWCYLDPKLGAMHAVAESARNVACTGATP
                     VAATNCLNFGNPEKPHIMWQFSQTIDGISKACEELETPITGGNVSFYNETLGEGIFPT
                     PVIGVVGIMEDVHKAIGPNFREAGRALLVIRGAEPGDASDVEAEFGSSEYAKEVLGEI
                     WGFPPSLELEKEAALQKAVVEMIALGLVESAKDCSEGGLAVTLTECGFAHGIGAEVNL
                     SSNGLVPEFVLFGEDASRVLISCDPRKLQGIQQIAVQYGLSAEPIGNTGPDQLEIQVD
                     GKAAVKASVSELQEAWASALERALHVETEERLVPSTLQKS"
BASE COUNT          590 a         1001 c          937 g          688 t
ORIGIN      
        1 gtccagggtc tgctcgcgcg aacgcgcaat gaggtggacc acaacctcgg tgtcgctggt
       61 ggtctgaaag atggagcccg cttggtcgag gtgggaccgc acttcagccg cattggttag
      121 attgccgttg tgcgccagcg cgatctttcc cttgttgcat tccaccatga tgggctgcgc
      181 attgagcagc gcggaatcgc ccgtagtgga gtagcgcgtg tgaccgatgg cgagcgaacc
      241 tggaatgagg gccagtacat ccgccgtgaa aatgtcggag accaagccca tggccttgtg
      301 cagttgcaac tgttctccgt tggatgccgc gatgcccgcc gattcctggc cgcgatgttg
      361 cagcgcgtac aggctaaggt aagcgagctt ggaggcttca ggatggccgt agatggccac
      421 cacgccgcat tcttctcgta gcttatcgga aggaatcagc atcgctcacc tgatgtcggg
      481 gctattgacg ctgcataagc aatgtccggc tgcgcgattt tgagttgtca tcacgaacgg
      541 ccttcaggcc gtgagggatc tgctgtttcc tcagtggtcg ttccagcgcc aacaaaagca
      601 gactccacgc ttcgctcgta atgacaactt cagagacgaa ccgtccatcc catatgggac
      661 attacttaca cagcgatcta tgaagctccc aaattctgta aatcgccgaa ccctgttcta
      721 ccctgtgccc actcgctagc tcttctgcaa cgtggaaggc accagccgtt cctcggtttc
      781 cacgtgcagg gcgcgttcca aggcactggc ccatgcctcc tgtaattcgg agacgcttgc
      841 tttgacggcg gcttttccgt caacctgaat ttccaattga tcgggacccg tattccctat
      901 cggttccgct gagaggccat attgtacagc tatttgttgg attccttgga gctttcgcgg
      961 gtcgcaggaa atgagcaccc ggctggcgtc ttctccaaag agcacgaact ccggcactaa
     1021 cccgttggaa ctaaggttga cctcggcgcc gatgccatgg gcgaatccgc attccgtcag
     1081 cgtcaccgcc aagccgccct ccgagcagtc tttggccgat tcgaccagcc ccagtgcgat
     1141 catttccacg accgctttct gtagcgccgc ttctttctcc aactccagcg acggcggaaa
     1201 gccccaaatc tcgcccaata cctcttttgc gtactccgag gagccgaatt ccgcctcaac
     1261 gtccgacgcg tctcccggtt cggccccgcg gatgaccagc agggcgcgtc ccgcctcgcg
     1321 gaaattgggg ccgatggcct tgtgcacatc ttccataatt ccgacgacgc caatcacggg
     1381 agtcgggaag atgccttcgc cgagcgtctc gttgtagaaa ctgacattgc cgccggtgat
     1441 gggggtttcc agctcttcgc aggccttgga gatgccgtcg atggtttgcg agaactgcca
     1501 catgatatgc ggcttctcgg gattgccgaa gttcaggcaa ttggtggccg cgaccggcgt
     1561 tgcgccggtg cacgctacgt tgcgggccga ctccgccacc gcgtgcatgg ctcccagctt
     1621 gggatcgagg taacaccagc ggctattgcc gtcgagcgcc atcgccaagc cacgccggat
     1681 acccttgatg cgaatcactc cgccctcgcc gcccgggcct tccaccgtat tgatttgcac
     1741 catggagtcg tattgctgga agacccagcg cttggagcag atgttcgggc tggccagcaa
     1801 tcgcttcaga tcgtcggtga attccgtttt ccggcccaac tcaatgcttg ccggaacctc
     1861 gcgcggaacg ggcggctccc agcgttccag cggccgatgg tagagcggcg catcgtcggt
     1921 cagcgccgcg ttgggaattt ccgcgacgac tttgccgtgc tccaacaccc gcatggcggg
     1981 ctgatcgatc acgcggccga tggttacggc atcgagtccc cacttctgga acacgcggaa
     2041 gacttcatcc tcgcgccctt tctcggccac cagcaacatg cgctcctgcg attcgctcag
     2101 catgatttcg tacggcgtca tgccggtctc gcgctggggc accaggtcga gctcgatttc
     2161 cagtcccacg ccgccgcgcc cgcccatctc gcaggtcgag caggtgaggc ccgccgctcc
     2221 catgtcctgg atgccgacaa ctgcaccggt ctgcatggct tccaggcagg cctccagcag
     2281 caacttctct aaaaacggat ctccgacttg gacattagga cgcttggctt ccgattcttc
     2341 gctgaactct tcgctcgcca tggtggcgcc gtgaatgcca tcccggccag ttttggagcc
     2401 gacgtagatc accggatttc cttcaccggc ggccttggca tagaagatct ggtcgcggcg
     2461 caccagtccc agcgcaaacg cgttgaccaa cggatttccc gaatagcagc tttcgaactt
     2521 cacctcgccg ccaatgtttg gaacgccgaa gcagttgccg tatgacgcga cgccgctcac
     2581 cacgccttct agcacggagt ggttcttatg cagcgtggtt tggtcgagac cggcctgcgg
     2641 cgtgatggga ccaaagcgca gcgaatccat gatggcgacg ggacgcgcgc ccatggtgaa
     2701 gatgtcgcgc agaatgccgc ccacgccggt tgcagccccc tgaaacggct cgatgaacga
     2761 aggatggttg tgcgactcga ttttgaaggc gcaggcccag ccatcgccta tgtcgatgat
     2821 gccggcgttc tctcccggac cttgcaccac gcgacggctg tgcgtgggca gccgcttcag
     2881 gtgcacgcgc gaggatttgt aggagcagtg ctcgctccac atcacgctaa agatgcccaa
     2941 ctcggtaaac gtaggctcgc ggccgagaga cttcaggatg cggtcgtatt cctccgctga
     3001 aattccgtgg tcgcgaagcg cttctggagt gatggtgggt tctttggcga ggctggtagc
     3061 gctatctact gggtacatgg cgaggaagga agttccattg tcgcacgctc catcggctta
     3121 gggaaggact tcggcagtac acgatttgcc tgtgcgaacc ggtggatctt gtcagggact
     3181 acgcgccgcc cccatcggag gtgctctgcg gtccgc
//
LOCUS       DASRKK010000195         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1284750, whole genome shotgun sequence.
ACCESSION   DASRKK010000195 DASRKK010000000
VERSION     DASRKK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284750"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFC15_04280"
     CDS             complement(<1..270)
                     /locus_tag="VFC15_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09408.1"
                     /translation="MGSRRPKYQSFEKDPSSIGEPSFIDDVLSYMNSPRGELSAEARE
                     IVWGLLERADVDAKNRQIIWNDGKRLSISASAQRIHDEHTDFPIDL"
     gene            complement(294..605)
                     /locus_tag="VFC15_04285"
     CDS             complement(294..605)
                     /locus_tag="VFC15_04285"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM09409.1"
                     /translation="MKTTRLDQIRKRIERIKAELVAIDEMRPGSLTEQLKKPDAQRGY
                     YQLSYTHGGKSRTEHVPRDCVGEVRRQIVNYKRFKALIAEWIDLSIEQCRLQIQSGSE
                     T"
     gene            674..2074
                     /locus_tag="VFC15_04290"
     CDS             674..2074
                     /locus_tag="VFC15_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09410.1"
                     /translation="MPLWVLWWNALQTVRPAFTRLRTFLWFATVVAGFAVRTEHLGVT
                     SIVRALKLRPKCYDALLDNLHSSAVRLDELSALWTRAVLRLFPNPLRVNGRRVLVGDG
                     IKAPKRGKKMPGVKLLHQQSECNNKPEYIMGHSLQAVGVLVRVANSVLAVPLAIRIHE
                     GLVWSNRDRRTLLDKMLALLNIVAVPDPFYFVGDAYYAAGKIAIGLLQNNNHLITRVK
                     SNAIAYEPYVAQGPKKRGRPRLYGPKIALRTLLTDLVGMQQAPSPVYGEQNTTLNFHV
                     RDLLWRPTGRLVRFVAVVHPARGSCLLMSTDTTLSAIDIIRTYGLRFKIEHTFKQAVR
                     LIGTFSYHFWMKAMKPLRRGSGNQYLHHKSAEYRDAVKRKTNAYHLFVQAGVISQGLL
                     QYLAAVASTQVWSSFGSWLRTIRPGVAPSELVVASALRQTLPEFLLNSVETASIAKFI
                     VERQDHERMEMFSVAS"
BASE COUNT          451 a          623 c          586 g          500 t
ORIGIN      
        1 caaatcgatc ggaaaatccg tatgctcgtc gtgaatccgc tgcgctgatg ccgaaatcga
       61 aagtcgctta ccgtcgttcc aaatgatctg acggtttttc gcatcgacgt ccgctcgttc
      121 gagcaagccc catacaattt ccctcgcttc ggcggagagt tcgccccgcg gtgaattcat
      181 atagctgagg acatcgtcaa tgaacgacgg ctcgccgatg gacgatgggt ctttctcgaa
      241 cgattggtat ttcggacggc ggcttcccat gcgcgaagta taacacaacg tttctaggtt
      301 tcgctaccgg attggatctg caagcgacac tgttcgatgc tcaagtcgat ccattctgca
      361 attagcgctt tgaaccgctt gtagttgacg atctgacgcc gaacctcgcc cacacaatcg
      421 cgagggacat gttcggttcg acttttgccg ccgtgcgtat aacttagctg gtagtagccg
      481 cgctgagcgt cgggtttttt caattgctcg gtcaacgatc ccggtctcat ctcgtcgatg
      541 gcgaccaact ctgccttgat ccgttcgatg cgtttgcgaa tctgatcgag tcgcgtcgtt
      601 ttcatgttgc tattgtatca tactaatgct acaatagcaa caattacacg gcaacaaaag
      661 atcggagatt ccggtgccgt tgtgggtctt gtggtggaat gcgctgcaaa cggtgcggcc
      721 agcgttcacg cgactgcgta cattcttgtg gttcgcgacg gtagtggccg gctttgccgt
      781 gcggaccgaa catttgggcg taacgagcat cgtgcgagct ctcaagctgc gccccaaatg
      841 ctacgacgcg ttgctcgaca acttgcacag ctcggctgtg cgcctcgatg aactcagtgc
      901 gctttggaca cgagccgttt tgcggctatt ccccaatccg ctgcgtgtca acggccgacg
      961 cgttctggtg ggagacggca tcaaggctcc caaacgcggc aagaagatgc cgggggtcaa
     1021 actgctgcat cagcagtccg agtgcaacaa caagcccgag tatattatgg gccactcctt
     1081 gcaggccgtc ggtgtgctcg tccgggtcgc caacagcgtc ctggccgtgc cgctggcgat
     1141 ccgcattcac gaagggctgg tatggtccaa ccgcgatcgc cgcacattgc tcgacaagat
     1201 gcttgcccta ctcaacatcg tcgcggttcc tgacccgttc tactttgtcg gcgatgccta
     1261 ctatgccgcg ggcaagatcg ccatcggatt acttcagaac aacaatcacc tgatcacgcg
     1321 tgtaaagtcc aacgcgattg cctacgagcc gtacgttgct caggggccca aaaagcgcgg
     1381 tcggcctcgg ctctatggac cgaaaatagc gctcagaaca ctgctcaccg atcttgtggg
     1441 catgcaacag gcgccaagcc ccgtctatgg cgagcagaac accacgctca acttccacgt
     1501 gcgggatcta ctgtggcgcc caaccggtcg actcgtgcgt ttcgtcgccg tcgttcatcc
     1561 cgcacgggga tcgtgcttgt tgatgtccac ggacacaacc ttgagcgcca tcgatattat
     1621 ccgcacatac ggtctgcgct tcaaaataga gcacacgttc aaacaggcag ttcgcctcat
     1681 cggaacgttc tcatatcact tctggatgaa agcgatgaaa ccgctgcggc gcggaagtgg
     1741 caaccagtac cttcatcaca agtcggccga gtatcgcgat gctgtcaagc gcaagacgaa
     1801 tgcttatcat ctgtttgtcc aggccggtgt tatctcgcaa gggctgcttc agtacttagc
     1861 ggccgtcgct tcgacacagg tgtggagctc ttttggctcg tggctgcgta ccatccgacc
     1921 cggtgtcgct ccatcggagc tcgtcgtcgc gagcgcgctc cgacagaccc tgccggaatt
     1981 tctcttgaat agcgtcgaaa cagcttccat cgcgaaattc attgtcgaac gccaagacca
     2041 tgagaggatg gagatgttca gcgtcgcgtc atgacaagaa cttgccactc acgaggatag
     2101 aaggttccct gcagataatc ggcgaggcgc tcaagcggta cgtttacggg agttgtcttc
//
LOCUS       DASRKK010000196         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_698536, whole genome shotgun sequence.
ACCESSION   DASRKK010000196 DASRKK010000000
VERSION     DASRKK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698536"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFC15_04295"
     CDS             complement(<1..872)
                     /locus_tag="VFC15_04295"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZM09411.1"
                     /translation="MATAQTREQESNPWEQQAARFNLAAEKLKLDQGLWRVLQQPNRE
                     IIVHIPVAMDNGQLEVFTGYRVQHSIARGPAKGGVRYSPDVTLDEVRALASWMTWKCA
                     VVNIPFGGAKGGIICDPKNMSRGELERMTRRYTSELIEFIGPEKDVPAPDVNTNEQTM
                     AWMMDTYSMHMRQTVTAVVTGKPLNMGGSRGRREATGRGVMIICDESIKKNNMTREST
                     RVIIQGFGNVGSNAAVLMHKAGYKVIGIGEWDGGLYNSKGIDITALWEHRQKEGTIHG
                     FKGAEPAATEDLLIT"
     gene            complement(1844..4918)
                     /locus_tag="VFC15_04300"
     CDS             complement(1844..4918)
                     /locus_tag="VFC15_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM09412.1"
                     /translation="MDSERWKQVDGLLQSVLERSPEERETFLRQACAGDEALEREVQS
                     LLSAQQEAEGFLDRPAIEVAARELACNENKVADESARSFIGQRVSHYQVIEKLGGGGM
                     GVVYKAEDTDLGRFVALKFLPADLAQDQQALERFRLEARAASALNHPNICTIYEIGKH
                     NDQSFIAMEFLDGMTLKHCIAGRPLETEALLALAIEIADALDAAHAAGIVHRDIKPAN
                     IFVTQRGHAKVLDFGLAKILKSKNIGVDASTIEKSLTTPGTAMGTVNYMSPEQVRAKE
                     LDARTDLFSFGAVLYEMSTRTLPFRGESTGVIFDAILNRTPVSPTRLNPDLPAELERI
                     VLKCLEKDRNLRYQHASDLRTDLQRLKRDSDSGIAATSAKPVASTGVARRWKAIALAV
                     VAVLALSVSSYFYFRQSPKLTNKDTIILADFTNSTGDAVFDGALRQGLSVQLQQSPFL
                     SIVPEQQVQQTLQMMGQKRDARLTTEIARELCQRTGSAAVIDGSITQIGTPYLLTVKA
                     VDCARGESLASAEAQASDKNHVLDALGKTASDIRKKLGESLSTVQKFDTPLEQATTPS
                     LEALKSFSSGFQIQMTAGDAAAIPFYKQAIELDPNFALAYAWMGIGYNDMGELSMDVE
                     STRKAFDLRDRTSEPEKYFITARFHKVVTGNLKKAEEALQLWIRAYPRMAQPHTYLAG
                     AIYPNEGQYEKGVAAGREAIRLSPNFSPAYGLTIPDYVAMNRIDEAKATYEQALERKL
                     KNSFFPLSLYEIAFLQGDAAGMAQQVSASAGTPGLEATLLAHEADTAAYSGRLRSARE
                     FSRQAVDSAERAGDKEAAGTYSALSALREGLFGNADEAKRRATSAMRHPAGHDLQYAT
                     ALAWAYAGDDERAQKLTDNLGQSFPEATVVQFIYLPTLRAKLAVGKGKASEALETLRA
                     ALPYELGRTTYSTLAWTALYPVYARGEAYLAAHQGKEAAAEFQKILDHRGIVLNGPIG
                     ALARLQLGRACALQGDTPKAKAAYQDFLTLWKDADPDIPILKDAKAEYGKLK"
     gene            complement(4911..5564)
                     /locus_tag="VFC15_04305"
     CDS             complement(4911..5564)
                     /locus_tag="VFC15_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM09413.1"
                     /translation="MQTQAATKNSKVFLMGNLSQPQDDGTGGPPVGEVSELLRAWSDG
                     DRAALDSLTPIVYGELHRLAGRYMKGERPGHSLQTTALVNEAYMRLVDYKNMRWQNRA
                     HFFAVSAQLMRRILVDHARRRNLKRGGGVQHVSLDQTAIVGSERAADLVALDDAMNLL
                     AKLDPRKVRVVEMRFFGGLSVEETAEILKVSAVTVMRDWNTAKAWLYRELKARTGNG"
     gene            5669..>6353
                     /locus_tag="VFC15_04310"
     CDS             5669..>6353
                     /locus_tag="VFC15_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09414.1"
                     /translation="MKSRMLAWIAVISVFTLPAMPVRVLAQERQQQAQHLSHYIVTDL
                     GTLEGGTFSQPFFINRYGLVSGSSNLADGNQHAVLWLGKLKLDIGMPGLGGPNSIAFG
                     DNDGFQSAGEAETSTPDPNGEDFCGFRTHLTCLPFLWQDGKMIQLPTLGGNNGAATAI
                     SNRGEVAGFAENSIPDPGCPSPQVLHFEPVVWEKGVIHELPTYGGDPDGITYLINDNG
                     EVVGGSGTCA"
BASE COUNT         1268 a         1815 c         1843 g         1427 t
ORIGIN      
        1 gtaatcagca ggtcttcggt ggccgcaggc tcagcgccct tgaagccgtg gatggtgcct
       61 tccttctggc gatgttccca cagcgcggta atgtcaatgc ctttgctgtt gtacaagccg
      121 ccgtcccatt cgccgatgcc aatcaccttg tatccggcct tgtgcatgag gaccgcagcg
      181 ttggacccga cattgccaaa gccctgaatg atcacgcgag tgctctcgcg cgtcatgttg
      241 ttcttcttga tggattcgtc gcaaatgatc atcacgccgc ggcctgtggc ttcgcggcgg
      301 cctcgcgaac cgcccatgtt cagcggcttg ccggttacca ccgccgtcac cgtctggcgc
      361 atgtgcatgg agtaggtatc catcatccac gccatggtct gctcgttggt gttgacgtcg
      421 ggggccggga catccttctc cgggccgata aactcgatca gctcggaggt gtaacggcgg
      481 gtcatgcgct ccagttcgcc gcgcgacatg ttcttgggat cgcagatgat gccgcccttg
      541 gcgccgccga acgggatatt caccaccgcg cacttccagg tcatccagct cgccagggcg
      601 cgcacttcgt cgagcgtcac atcgggcgaa tagcgcactc cgcctttggc gggaccgcgc
      661 gcgatggagt gctgcacccg gtagccggtg aatacttcca gttgcccgtt gtccatggcg
      721 accggaatgt ggacgatgat ctcgcggttg ggctgctgca aaacccgcca taggccttga
      781 tcgagtttca gtttttcggc cgccaggttg aagcgcgcgg cctgttgttc ccaaggatta
      841 ctttcctgct cacgagtctg tgctgtagcc atagatttct ccggattatc gcgggcgttt
      901 tgcggcacga ttgggtggac gccttcctcg accagcatcc agcatcggcg ggcaaacctt
      961 caaagtatat gcagccggat gtctcccgcg caaggtgaac gaactatccg gaggtgtctg
     1021 aaaagtgcgt tgatgtgtgg agcagctttc ggcactactt gacgggagtg gagggtgtag
     1081 tggagattga atcggaatgg atgggacgca agcgggaacg catggggaca atcccgcgag
     1141 tgcgaatccg tccggctgtg cccaccctct cgccaaaaac gggcgagaag gatggggcac
     1201 ccgcaaattg agtgttacgg tcgctgacac tgaaaggggt gggccacccg cctactaaaa
     1261 gctgtgctga ataatcagta gagagggctg acatgacgtt tgaggaatta gaccagagat
     1321 atccaaatgg ttttgtcgac gcggagataa ccagcctgat tctggactac caaaatcgta
     1381 ctgcgacgtt acatctgaat ctgcgtggga actcgcccga tagccccgac cgcaatgtct
     1441 atgagcgagc ggtgcttatt gcgcgtgaat tttattattt ttcgatagaa ccgcctgatc
     1501 cggatcacct tttttacccc aagagatcga ggatagaagt aaatggatac tctgaagatc
     1561 cgagccaatt cccactattc gagcagctga aaccaaaact gtctgccggt gccttctgct
     1621 gtcggttttt cgttcacgat tggaactcat tcattcacat agccgcgagg gacgctcagt
     1681 tttcgtgggt cgaagatgga gcacgagcaa accccctggt gtccgattcc tgaagtcgac
     1741 aggtctggcg gttatcgtcg tggtcggacg ggcgggtggc cgggtgcccc acccggaatc
     1801 ggcggcaagg gtcaatcgag cttccacgag gggagtgttc cggttatttc agcttcccgt
     1861 actccgcctt ggcatctttc aggatgggga tgtcggggtc ggcgtccttc cagagtgtga
     1921 ggaaatcctg atatgcagcc tttgcctttg gtgtgtcgcc ctgcaaggcg caggcgcgac
     1981 caagctgcag acgcgcgaga gcgccaatcg gtccgttcaa tacgattccg cgatggtcga
     2041 gaattttctg gaactcagcc gcggcttctt tgccctgatg ggcggccaga taggcttctc
     2101 cgcgagcata gacaggatac agggccgtcc aggcgagggt gctgtaagtc gtccgtccca
     2161 gctcatacgg cagggcggct ctgagggtct ccagggcttc cgaagccttt cccttgccga
     2221 ccgcaagctt tgcgcggagg gttggcaggt aaatgaactg gacgaccgtg gcttccggga
     2281 agctctggcc caaattgtcg gttagtttct gggctcgctc gtcgtccccg gcataggccc
     2341 acgcaagtgc ggtagcgtac tgcagatcgt ggcctgccgg atgcctcatt gccgaagtgg
     2401 cacgccgttt tgcttcgtct gcgttgccga ataagccctc ccggagagct gaaagggcgg
     2461 agtaggttcc agccgcttcc ttgtctccag cccgttcggc ggaatctact gcctgacggg
     2521 aaaactctcg ggcactccta agccgcccgg agtaggcagc ggtatcggcc tcatgcgcga
     2581 gcaaggtagc ttccaggccc ggcgttcccg ccgatgctga tacctgctgc gccatccctg
     2641 ctgcatcacc ctgcaaaaag gcaatctcat acaaagaaag agggaagaaa gagttcttca
     2701 gtttgcgttc gagagcttgc tcatacgtgg ccttcgcctc atcgatgcga ttcatcgcaa
     2761 cgtagtcagg aatcgtgagt ccgtaggcgg gggaaaagtt cggactcaag cggattgcct
     2821 ctctgcctgc tgcaacccct ttctcatatt gtccttcatt cgggtagatt gcccccgcta
     2881 agtaagtatg cggttgtgcc atgcggggat aagctcgtat ccaaagctgg agggcttctt
     2941 cggccttttt caggttcccg gtgaccacct tatggaagcg agctgtgatg aagtatttct
     3001 caggctcgct ggttcggtcg cggaggtcga aagccttccg ggtactctca acatccatac
     3061 tgagttcacc catatcgttg tacccgatgc ccatccacgc gtaggcgagt gcgaaattag
     3121 ggtcgagttc gatggcctgt ttataaaacg ggatagccgc agcatccccg gcagtcatct
     3181 gaatttgaaa acccgaactg aaggatttca gggcttcaag cgacggggtg gtcgcttgtt
     3241 caagaggagt atcgaatttc tgcaccgtgc tcagcgactc gcccagcttc ttccgaatgt
     3301 ccgaagctgt tttgcccagt gcatcgagca cgtggttctt gtcgctcgcc tgggcttccg
     3361 cgctggccag cgactcgcct ctggcgcaat cgaccgcctt gacggtcaaa aggtacggcg
     3421 tgccaatctg cgttatcgag ccgtcgatga cagcggcgct gcccgtccgc tggcagagtt
     3481 cccgcgcaat ctccgtcgtg agcctcgcat cccgcttctg gcccatcatc tgtaaggtct
     3541 gctggacttg ctgctcgggc acaatgctga ggaatggcga ctgttgcagc tgcacggaca
     3601 acccttgccg cagagcgccg tcaaagactg cgtcacccgt tgagttggtg aaatcggcaa
     3661 ggataatcgt gtccttattt gtgagttttg ggctttggcg gaaatagaag taactgctaa
     3721 ccgataaagc cagcacagcc acgacagcaa gagcaatggc cttccaacgt ctggcgacgc
     3781 ccgttgaggc cacgggcttc gcacttgtcg ccgctattcc ggaatccgag tctcgtttca
     3841 gccgttgcag atcagtacgc agatccgacg catgctggta ccgcaggttt cgatctttct
     3901 ccagacactt aagaacgatc cgttccagtt cagcgggcag atcgggatta agccgcgtcg
     3961 gagagactgg agtgcgattc aaaatcgcgt cgaaaattac gcctgtgctt tccccgcgga
     4021 agggcaatgt ccgggtggac atctcgtaga ggactgcgcc gaaggaaaac aggtcggtac
     4081 gtgcatccag ctccttggcg cggacttgtt ccggcgacat gtagttcacc gtgcccattg
     4141 ctgttccggg agtggtcaga gacttttcta tggtggaggc gtcgacacca atgttcttgc
     4201 tcttcagaat cttggccagt ccaaaatcga gaaccttcgc atgtccgcgc tgggtgacga
     4261 agatgttcgc cggcttgatg tcgcgatgga caatgccagc ggcgtgagcc gcatccagtg
     4321 cgtcggcgat ttcgatagca agcgcgagaa gagcctcagt ttcgagcgga cggcctgcaa
     4381 tgcagtgctt cagcgtcatc ccgtccagaa actccatggc gatgaacgac tgatcattat
     4441 gtttgcctat ttcgtagatc gtgcagatgt tgggatggtt cagtgcagac gcagcccggg
     4501 cttcaaggcg gaagcgttct aacgcttgct ggtcttgggc tagatccgcg ggcaggaatt
     4561 tcagagcgac aaagcggccc aggtcggtgt cttccgcctt gtagaccacc cccatgccac
     4621 cgccgcccag cttttcgatt acctggtaat gcgagactct ctggccgatg aaggagcggg
     4681 cactctcgtc cgcaactttg ttctcgttgc aggcgagctc gcgcgcggcc acttcgatag
     4741 ccggacgatc gagaaatcct tcagcctctt gctgcgcact caacagcgac tggacttcgc
     4801 gctccagcgc ctcatcgccg gcgcatgctt gacgcagaaa cgtctcgcgc tcctcgggcg
     4861 aacgttccag cacggactgc aacaggccat cgacttgttt ccaccgttcg ctatccattc
     4921 ccggtcctgg ccttaagctc acggtagagc caggctttgg cggtgttcca gtcgcgcatc
     4981 acggtgaccg ccgatacttt caatatctcg gccgtctctt ccacactgag tccgccaaaa
     5041 aaacgcattt cgacgacccg cactttgcgc ggatcgagct ttgccagcag gttcatggcg
     5101 tcgtcgagcg ccaccagatc ggcggcgcgt tcgctgccca ctatcgccgt ctggtccaat
     5161 gaaacatgtt gtacgccacc cccacgtttc aggttacgac gccgcgcatg atcaaccaga
     5221 atgcgcctca tgagctgggc cgaaaccgcg aaaaaatggg cgcgattctg ccaccgcatg
     5281 ttcttgtaat ccaccaaccg catataggct tcgttgacca gagcggtggt ttgcaggcta
     5341 tgaccggggc gttctccttt catgtatcgt cccgcgaggc gatggagttc gccataaacg
     5401 atgggcgtaa ggctgtcgag cgcggctcgg tctccatcac tccaggctcg aagcaactca
     5461 ctcacctcgc caacaggtgg ccctccggtc ccgtcgtcct gcggttgtga caaattcccc
     5521 attaggaaca ccttggagtt cttagtcgct gcttgagtct gcaagaaagt tatgcctgca
     5581 tgatagttct atacccccgt ttgcgcctac ctatatgaga gcgaaaaacc cagcaggaca
     5641 cctgccggga cgcacgacgg aggagactat gaaatcgaga atgttagcgt ggatcgcggt
     5701 gataagcgta ttcactttgc cggcgatgcc ggttcgggtg cttgcgcagg agcgacagca
     5761 gcaagcacaa cacctttccc actatatcgt taccgacctc ggcacgttgg agggcggaac
     5821 ctttagccaa ccattcttta ttaaccggta tggtctagtg agtggctcgt cgaacctcgc
     5881 ggacggcaat cagcacgcgg tcctttggct ggggaagctg aagctcgaca ttggcatgcc
     5941 cggacttggg gggccgaaca gcatcgcgtt tggcgacaac gacggattcc agtcagccgg
     6001 tgaagctgaa acctctactc cggatcccaa tggtgaagat ttttgcggat tccgtaccca
     6061 cctcacctgt ttacctttcc tgtggcagga cgggaaaatg attcagctcc cgacactggg
     6121 tggcaacaat ggcgcggcaa cggcgattag caatcgggga gaagtggccg gatttgcgga
     6181 gaactcgata ccagacccag gctgcccatc tccccaagtt ctccactttg agcccgtcgt
     6241 ctgggaaaaa ggagtgattc acgaactgcc tacgtatggg ggcgatccag atggcatcac
     6301 ctacctgatc aacgacaacg gcgaggttgt cggcgggtcg ggcacttgtg caa
//
LOCUS       DASRKK010000197         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_14482, whole genome shotgun sequence.
ACCESSION   DASRKK010000197 DASRKK010000000
VERSION     DASRKK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14482"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFC15_04315"
     CDS             complement(<1..802)
                     /locus_tag="VFC15_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09415.1"
                     /translation="MADNKKGLVTAGFSLLWRRQAILWWVFVVSLVCGALGTAPAALQ
                     LNRALHHTFAGQPLTKGFDLGMFFELVRLPNVSLMRSTTSSYIFALLFFLFMLFVSGG
                     ILETYHQDRRLSTGDFFAASGAFFWRFVRLMLFSLVPFIFLWSLYAQVNRLSDYVGDR
                     AVADQVGFCIQFAGTVLLALLALLVRLWFDLAKVRAVAQSEHSMWHNMWRSCGMTWRH
                     LGTLFWMYLRISLAAWIALLIGLLIWTKLPPTAIPATFILLEFIILSQL"
     gene            complement(815..2767)
                     /locus_tag="VFC15_04320"
     CDS             complement(815..2767)
                     /locus_tag="VFC15_04320"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZM09416.1"
                     /translation="MQRQRALLLFAVGLLCHTSAIAQSPFAAKVSGPLSQRVVAYQID
                     AKYDPARHTVEATETLTYRNLTGQPLDRFPFHLYLNAFQQKSTWMHEAHRDGSFRTSS
                     LDEWKPKDYGANDVTSFEVVGMGDLTKQMKFISPDDGNPNDKTVFEVKLPHAIQPGQD
                     VQFKIKFKATFPEVIARTGYKRTFLLAGQWFPKVGVWWHGAWNCHQFHALTEFFADFG
                     TFDVKVTLPKDYVIGATGVQTGDADNGNGTKTVSFHAEDVHDFAWAADPNFKVVEGQF
                     DGSVGPVRIRLLTYDSHKSSWQPYIYSAKESMKRFDEWYGPYPYAQLTVVDPPHGAGE
                     AGGMEYPTFITGDSGWFIPKGVHLIDLVTEHEFGHQYWYGMVATNEFENGWLDEGINS
                     YTEMKVLDNMYGENASAINLLGAQLGDRGLQRMSYLDVADTDSLSRSSYTDMSIGTYG
                     GVTYGKTATMLVSLEAIVGEQTLKNALHTYFMRYRFTHPTQEDFMRTVNEVAGRDLQW
                     FWTQAVYGTQVLDYEVLRADSNPTNWYDENATEKKGETTYETQVILHRKGDFVFPVEA
                     EVKFDNGESARERWDGKDRWVRYVYRKKAQVESVQIDPDYQVTLDRDYLNNSQVTKSQ
                     RGATAKIATYWMFLTQFLAQFLSWLA"
     gene            complement(2798..>3188)
                     /locus_tag="VFC15_04325"
     CDS             complement(2798..>3188)
                     /locus_tag="VFC15_04325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZM09417.1"
                     /translation="DVDAAHGAPVVTDMGKDEFGHVRLGGIGQILAHELEHRTGLETR
                     NVVLGHIQRGGTPSAFDRVLASRYGIGAIDQVHKAEFGVMVALRGTDIVTIPLVDAIH
                     KTRPVDPELVEVAMGLIDPIPTAPANK"
BASE COUNT          671 a          954 c          903 g          660 t
ORIGIN      
        1 ccaactgcga cagaataatg aattcgagaa ggatgaacgt tgccggaatc gcggtgggcg
       61 gcagcttcgt ccagatcagc aaaccaatca ggagcgcgat ccaggcggcc aggctgatgc
      121 gaaggtacat ccagaacagc gtgccgaggt ggcgccaggt catgccgcag gacctccaca
      181 tgttgtgcca catactgtgt tcgctctgcg ccacggctcg aactttggca agatcgaacc
      241 acaaccggac caggagcgcg agcagtgcga gaaggaccgt gcctgcaaac tggatgcaaa
      301 agccgacctg atcggcgacc gctctgtcac ccacgtaatc cgagagcctg ttgacctgcg
      361 catacaacga ccacaggaaa ataaagggca ccagtgagaa gagcatcagc cgaacgaagc
      421 gccagaagaa tgcgcccgag gcggcaaaga agtcgccggt gctcagcctg cggtcctgat
      481 gataggtctc gagaattccg ccgctgacga acagcatgaa aaggaagaac agcaacgcga
      541 agatgtagga gctggttgtc gagcgcatca ggcttacgtt cggcagcctc accagctcga
      601 agaacattcc gaggtcaaag cctttggtga gaggctgtcc ggcaaaggtg tggtgcagtg
      661 cgcgattcag ttgcagcgct gccggagcag tccccagcgc accgcagacg aggctcacca
      721 cgaacaccca ccacagaatt gcttgccggc gccaaagaag gctgaacccg gcagtgacca
      781 agcccttctt gttgtccgcc acggtccctc ctttctaagc cagccagctc agaaactgag
      841 ccaggaactg cgtcaggaac atccagtagg ttgcgatctt cgcagtcgca ccgcgctgcg
      901 acttggtgac ctggctgttg ttgagatagt cgcgatccag cgtgacctgg tagtcaggat
      961 cgatctgcac ggactccacc tgcgctttct tgcgatacac gtatcgcacc caccggtctt
     1021 ttccatccca ccgttcgcgg gcgctctctc cgttgtcgaa cttcacttcg gcctcgaccg
     1081 ggaagacgaa gtcccctttc cgatgcagga tgacttgcgt ctcgtaggtc gtctcgccct
     1141 tcttctcggt cgcgttttcg tcgtaccagt tcgtgggatt ggagtcggcg cgcagcacct
     1201 cgtaatccaa tacctgcgtg ccatacacgg cctgggtcca gaaccactgc agatcgcggc
     1261 cggcaacttc gttcaccgtt cgcatgaagt cttcctgcgt gggatgggta aaccggtacc
     1321 tcatgaagta ggtatgcagc gcatttttca gcgtctgctc gccaacgata gcttccagcg
     1381 agaccagcat cgtggctgtc ttgccatacg tgacgccgcc gtatgtgccg atgctcatat
     1441 cggtgtagct ggatcgcgag agcgaatcgg tgtcggccac gtcgaggtaa ctcatgcgtt
     1501 gcagaccacg gtcgcctaac tgtgctccca gcaagttaat ggcgctcgca ttctcgccgt
     1561 acatattgtc cagcactttc atttcggtat aactgttgat cccctcatcc agccacccat
     1621 tctcgaattc gttggtcgcc accatcccat accagtactg gtggccgaac tcgtgctccg
     1681 tgaccaggtc aatcaggtgc actcccttgg ggatgaacca accgctgtcc cctgtaatga
     1741 acgtggggta ttccatgccg cccgcctcgc ccgctccatg aggaggatcg accaccgtga
     1801 gctgcgcgta ggggtaaggg ccgtaccatt cgtcaaagcg cttcatgctc tctttggccg
     1861 agtaaatata aggctgccat gaactcttgt ggctgtcgta ggtgagcagt cgaatcctca
     1921 ctgggccgac actgccgtcg aactggccct cgacaacttt gaaattcggg tcggcggccc
     1981 aggcgaaatc atgaacatct tcggcgtgaa agctcacggt cttggtacca ttgccgttat
     2041 cggcatcgcc cgtctgcacg ccggtggcgc cgatgacgta atccttcggc aaggtgacct
     2101 tcacgtcgaa ggtgccgaag tcggcaaaaa actcggtcag ggcgtggaac tgatgacaat
     2161 tccacgcacc gtgccaccac accccgacct tgggaaacca ctgcccggcg agcaggaaag
     2221 tgcgcttgta cccggtgcgg gcaatcactt caggaaaggt cgccttgaac ttgattttga
     2281 actgcacatc ctgccccggc tggatcgcat gcggcaactt cacctcgaac accgtcttgt
     2341 cattgggatt accgtcgtcc ggggagatga acttcatctg tttggtcagg tctcccatgc
     2401 cgacgacctc aaaggaagtc acgtcgtttg ccccatagtc cttaggtttc cactcatcca
     2461 ggctgctggt gcgaaagctg ccgtcgcggt gcgcttcatg catccaggtc gacttctgct
     2521 ggaacgcatt cagatagagg tgaaaaggaa atcggtcaag cggctgacct gtgaggttgc
     2581 gataggtgag cgtctcggtg gcctcaaccg tgtgcctcgc gggatcgtac ttcgcatcaa
     2641 tctggtaggc cactacccgc tgtgacagag gcccggaaac tttcgcagca aaagggcttt
     2701 gcgcaattgc cgaagtgtgg caaagcagtc caaccgcgaa caacaacagc gctcgttgac
     2761 gttgcatcgc tgatctccca gacattcctt attgtgctta cttattcgcc ggggcggtgg
     2821 gaatgggatc gatcaatccc attgcgactt ccaccagttc cggatcgaca gggcgagttt
     2881 tgtgaatggc atctaccaac ggaatcgtga caatgtcagt cccgcggagc gcgaccatga
     2941 ctccgaattc ggctttgtgg acctggtcga tggcgccgat tccgtagcgc gacgccagca
     3001 cgcgatcgaa cgcgctgggc gtgcctccgc gctggatgtg cccgagcacg acgttgcggg
     3061 tctccagtcc ggtgcgatgt tcgagttcgt gcgccaggat ctggccgatg ccgcccagac
     3121 gcacgtgacc aaactcgtct ttgcccatgt cggtgaccac cggggcgccg tgcgcagcat
     3181 cgacatcg
//
LOCUS       DASRKK010000198        16861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1284879, whole genome shotgun sequence.
ACCESSION   DASRKK010000198 DASRKK010000000
VERSION     DASRKK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16861
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284879"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFC15_04330"
     CDS             <1..553
                     /locus_tag="VFC15_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09418.1"
                     /translation="QNWSYEQQIGLLSNTTYGSQKIPTLLGNDGTTAGNYTNDHPVGV
                     NATISSGSGLVWANNSFTVTPGTPFAQFVANYGWPALAPGKWSSPWGVNGAGQPYVLC
                     TTCHNQHVMTVYTSSKTSQIVGDGGGKYYATYFFVNGPYNPNLQGKLTAGQAASTTQF
                     CRQCHFGEANEANNTNNIPTVFQ"
     gene            628..1647
                     /locus_tag="VFC15_04335"
     CDS             628..1647
                     /locus_tag="VFC15_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM09419.1"
                     /translation="MKYQLITALLLTASVVAPAQVASHAPSTAAKLPPAQVAPLTPTG
                     KPVAKVNGTVLTDKDLLREMYAIFPYAKQHNGFPKAQEAGIRQGALEMIIFEELVYQE
                     ALRRKMTIPAAKIDQGEAAYKKTFDSPEQYQQFMQVEMHGSRQQLRDQIQRSLLIDQL
                     LKQEVDNKAIVSQAEVKAYYDKNSARFQLPESYTFQAISILPPQTATPAQAKEARKRA
                     DEALKQAKATASYQEFGLLAEKISEDDFRVNMGDHKIVPADKLPPQVIKAFQAVKPGQ
                     VSGLIQIENAYTIVRLNAHTPAHKQSLTEVQAQLKTELQKQKFEQLRSNFAKQLRAKA
                     KIEMA"
     gene            1913..3763
                     /locus_tag="VFC15_04340"
     CDS             1913..3763
                     /locus_tag="VFC15_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09420.1"
                     /translation="MSVMKSVRTLGWIVVLALAPWTHAQVQIGDNTHVNAGGLFTVGY
                     AGDYGNQIQSSHGLQFGASGTMSGYYYNPNFVNFNVTPYYNQSKADSDYQSLTNASGV
                     AATANFFTGSHFPGSVTYRYDYNSTGTLGLSGVPNFTTQGNGQGFGINWSALLPDLPT
                     LTVGYQQGNGSSTLYGTNQESSSDQRLFNVRSSYRLEGFNINAFYDHTTLNSVYPEFL
                     TGVGQEINDSSGQDFGVNTSRNLAWMNGSMYASYSHSSYSTDYGTSGQSSTNSGYTAD
                     LESAGVNFHPTQKFSFYANENFTNNLSAYFNPALVSGGVVTPPVNLLGSGSDSLTAGG
                     GANYQFTGHLNGNAQATYYSQSYFGQTYTGTYLSGNVNYGRKLLNMFTFSAGVVDSST
                     GLGNNNIGFIGTVNYFRRFGGWETSGSFNYAQNVQSLLITETTSYYNYNANIHRKFSS
                     RVQWTAAFNGSRQGLSSNKNSSSSSEGFATSLSLRQVALSAQYLTGTGNSILTSNGLT
                     PLPPLPGVTPENVIAYNAKSYGGSVSFTPVRRMVLSGTYSRSLSDTLSNGIFSKNNTE
                     IIYSQLQYRLRRISLLAGYSRFTQGISATGVPPGTVTSYYGGISRWFDFF"
     gene            3775..4848
                     /locus_tag="VFC15_04345"
     CDS             3775..4848
                     /locus_tag="VFC15_04345"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZM09421.1"
                     /translation="MRIQRVVMWMGLALLLLSPALASKQPKQPLNAPEGPELLLDGGR
                     KLTWERSFSLEREVKPKRSFWTKLVDVIAGEPDFRYLVNPYSVTTDSRGRIIVSDPGA
                     NGVHIFDFNQQKYKFLTRKEKDKDGTLTPQCVAVDAQDNIYVTDSQAGKIFVFDANGK
                     YQHAIGSLKGGEGFFKRPTGIAVDSAAQRIYVTDTLRNKIFVMDMQGSVLQTIGKEGT
                     GDGEFNYPTELRLNGNDLVVVDAMNFRVQVLDRSGSFRYSIGKLGDGTGWMFRPKGIG
                     FDSEGHLYVVDGAWGVVQVFNQEGQLLYYFGQKGSGPGEFQLPAGLQIDNSDHIYVVD
                     SYNRRVQVFHYQGLPRQAAGGKP"
     gene            4845..6767
                     /locus_tag="VFC15_04350"
     CDS             4845..6767
                     /locus_tag="VFC15_04350"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM09422.1"
                     /translation="MKFLLRVSSLALLSLGIAFSQQPPSDVLGMHNLSPGSGASIYVP
                     SGNLGCQYCHAPHSGLGGVTPLWNQQLSKSSYTPYVSTTYHQVGNTQPPLGVTSSLCL
                     SCHDGTVAVGQTAAYGKVTTTGSWAPGDSFGNDLTSSHPFSLVKPIKDTADLAASLVS
                     QGTTADPLHKVKLINGNVECTSCHDPHTQGIDQIAQNFLVRDSSSGQMCLACHDPNRV
                     VQGQINPLSSWPSSIHQAAANKVSTSAHVGSYVTVAQNSCSSCHVEHNANGPARLLRQ
                     ATPPAPGVDASTQDCMTCHSGGTYVSPAAPNITAEMAKTGHPLPSGNNNHDAAEPAVL
                     INNRHATCVDCHSAHGSSQVVTFGAPPTMRVSQSGVQGISALDGITVLTPAVNQYENC
                     LRCHGTGAGKQRRIMYGYAPARLVTMPDFLNVINEFSATASSSHPVTHDRSSPLPQPS
                     LRINMLTLSGQPSARATGPRIFCTDCHNSDDNREFGGSGPNGPHGSANSHILERNYQF
                     SQASIPGALVTNTFPNPDLSVNGPYAMCAKCHDLTQVLANTSWVKHNSHVSQDGFTCS
                     VCHTAHGMGANSPNVTGERLVNFDVNVVGSNNGLPISYNRGTGNCTLTCHQVAHNGDG
                     TTSSALKVGPGRLKRN"
     gene            6770..8257
                     /locus_tag="VFC15_04355"
     CDS             6770..8257
                     /locus_tag="VFC15_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09423.1"
                     /translation="MTRPQFGTVPQLFALFVLFAAAGFHESIRQACLNSITNGDFWWH
                     LRTGLGILASQAVPHSGVYSQSASLPWTASSWLYDVLVAVGFRLLDLRTPAVLAIVFK
                     FALALLTFLLAGGLRGKFWTAVALSTAAQYILGAVPPLPLYCSVLAFAVELLLLMQCR
                     ATGNARPLYWLPLLFLLWANLDVHFVLGIVLLLLFVAACAIEQWGTRARMVWLVRSSR
                     PPLNALAAVTLASLVATVITPYGWNLYGLFLARVTSAANIYFPGFQSLRFRSPQDYLL
                     LLLVMAAFLALGMRRSRDLFQIALLLLCTMAAFHAQRDAWLVTLAAVAVVGNAVPQTN
                     LQSGREDARIPARQFFSAAALALVLLAIATAIHLPHGRQGLLAEIGEGYPVAAADYIR
                     EHQLPQPLFNSFPWGGFLTWYLPEYPVAIDGRTDLYGDDFNIQYAKVMNAEAHYSTFP
                     PLARAGTILLEKKSLMGSALQHVAGFNQVYADDVAVVLLREQPQP"
     gene            8254..9759
                     /locus_tag="VFC15_04360"
     CDS             8254..9759
                     /locus_tag="VFC15_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09424.1"
                     /translation="MSATATVTAVPQPTAPPKWESIGNYMVLLAYSAVVLFTVRYHEK
                     WADEAQAWLIARDLDLKTIWFHELRYEGHPGLWHTILWIAQHVFHAPYAALGYIGVAF
                     AIAGVAVLVLKAPFPWFIRWPLAFTYVLVYQYAVIARSYTILPLLCFLVAILFKDIEH
                     PERMTIVLVLLTMLTLHGAILAACYGLLYLVEAIKSWPQLNVRLRDRYFICAAVLTFA
                     FLFLVVILKPTPDVGEFATKRALDAAPEAVKATFPTALAKLTAVISGAFLDFWLSSTI
                     FLVLAGAWCYTRRRLVLYILPMGLLIALYAKVHGYPHHHGTVFIAAITAFWIAWPSED
                     ETRSFNVRQHRAMHGMIALLLCLFAVNIWDAAVVIQREYRYPYSGSKDAAMYLKSVGA
                     DRGKVFGFLFGVNAVQAYFDHNILANIPTAYYHNGLPLVGDTLNVAELNRVRPEYIVA
                     YSGEPQLMIRVGIPELESRGYKMVHFSDGYYMYKQAVYQRESYFILRRVQP"
     gene            complement(9881..11362)
                     /locus_tag="VFC15_04365"
     CDS             complement(9881..11362)
                     /locus_tag="VFC15_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09425.1"
                     /translation="MSGQQDLRSLLRFRPLQLLGLAAFLAAIGGMSFYTKLCVIDNDI
                     WWHLKVGDWMVEHLAVPHTGILSRTAATRPWVAYSWGYEVLMSLVYKWFGLMGIGLYG
                     TLITILVAYAVYWMLRRISGRFWVALVLAALTCWAFLFNGMPRPVFFSIALFCVTLAL
                     LLEANRTGRVELLYWLPLIFWVWGNLHIQFIYGLFLIGLLVGTTLAQRIADSWGIAPS
                     FLVPATLPSLPLLGVFASCLLATLIGPNFYHPYIAVYEYSKAKFAYNVIIELQPLSFR
                     GSSHYVQLLLTACGFFAVGWRKKIDLFKLAMMTACSLVAYRTMRDAWFVAIPAAACIA
                     DFPAAEAECDAAENWMENLGVAAAVVVLLLVISRGTDFTSRDLDRAISSEYPVNAVNF
                     LRRNPQPGPLYNNLGWGGFLMWYMPDYPVAIDGRNDLYGDELDQLFYASQSAEAGYTT
                     DPYLNEAGVVVLAADLPLAKILTADPRFKLIYQDSLAVVFGRQ"
     gene            complement(11439..12959)
                     /locus_tag="VFC15_04370"
     CDS             complement(11439..12959)
                     /locus_tag="VFC15_04370"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM09426.1"
                     /translation="MDRKVYKSVIVGAGPAGLTAAYELSKLKQPVVVLESDPEYVGGI
                     SRTVRYKGFHFDIGGHRFFSKSREVEDLWTEIAGDLMLERPRSSRIFYRGQFYSYPLK
                     PFEALSKLGLFESFRCMASFAAARLHPTPNPKTFEDWVVNQFGERLFQIFFKTYTEKV
                     WGMSCSEISADWAAQRIKGLSLGSAIKHAILPQRKSKDRKNVVKTLIDTFRYPRQGPG
                     MMWESCANKVRALGGEVLLGRKVVGCRFDSQTKHWTISARTAVGAIEQFEAEHLVSSM
                     PIQELIAQLEPQLPPEALNAARSLRYRDFLTIGLILRDKDRFTDNWIYIHDSSVQVGR
                     VQNYKSWSPEMVPDPAFCCYGLEYFCFEGDGLWTASDNTLLAMAKKEIQQIGLGSAED
                     VVDGCVIRQKKAYPVYDDAYQTHVSTIRQALAEHCINLHLVGRNGMHKYNNQDHAMMT
                     AMLAAKNIAAGRELYDVWAVNQDGEYHEAGSAGEHSSGLRLVPEKPQEASLAPESR"
     gene            13291..13992
                     /locus_tag="VFC15_04375"
     CDS             13291..13992
                     /locus_tag="VFC15_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09427.1"
                     /translation="MDNEDNQPRTAGTNWTPTATYAMAAVCLLIGVLVGYVVRGSAPA
                     ASHPQMAASEGAAPATAPAMPAGMTGDAPHPAPTLDDMKRMADKKAEPLLAKLKTDPN
                     NAETLNELGILYRATHQFKEAEGYYEKSLQIDPKNVKVRTDLASCLYYTGDVDGALAQ
                     LNKSLTYDPKHAGTLMNIGIIEWQGKKDVNAAVAAWQKLLKLNPDFPQKDVVEKMIAE
                     AKGQGTTAQAESQTK"
     gene            complement(14032..15681)
                     /locus_tag="VFC15_04380"
     CDS             complement(14032..15681)
                     /locus_tag="VFC15_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM09428.1"
                     /translation="MTDSRSFDLYLEPWLNVRVPRMAHYSAQLADFRDWVLKEVDAQA
                     NYTDRYAPPSLETYDRNGEVVNRIVANPWYETQHQELYRRGIIGLPYVEDAPHVLTFA
                     MGYVLAQADISLHCPVTLTGAVAYVLGSRAPDDVRQRYLYDVVRMDGHAKTGGTWATE
                     QHGGSDVGATTTVAVPAGDHFALHGLKWFASNANSGLAVATARPEGAPPGSSGLGLYL
                     VPSHLEDGRPNHFRIRKLKDKLGTKGLPTGEIELLGAEAVEIAAPPLGFKLMMEALEY
                     SRVHNAVGSVGVERRALREALAWAKTRQAFGHPLSDYPMVQYELLRMRVQFEAGTLLA
                     FEAAITFDEVQQNTERRTWLRLVTALAKYLTAEYAITSARAALELIGGNGYTSDYPVA
                     RLLRDAQVLTVWEGPANIQALELLRLMAPRYQGWETYRKRAHEILDRTPDGLGNLRQA
                     LAQRLQGDSDALAITLRDEQSAPRFARKLLHRMSQSLAFALLCEVAGEAYAEGNSLPA
                     HSAWRFYEDIEPPTFGSENEAARRGVLELLEEESAKTTNPS"
     gene            complement(16020..16262)
                     /locus_tag="VFC15_04385"
     CDS             complement(16020..16262)
                     /locus_tag="VFC15_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09429.1"
                     /translation="MNGTLEKIPAILDLSCKTMRVIRQNLFWAFFYNALGIGLAITGV
                     LNPIMAAVAMLLSSASVVGNTWRLTARPFLHSNVLW"
BASE COUNT         3579 a         5155 c         4729 g         3398 t
ORIGIN      
        1 gcagaactgg tcttacgaac agcagatcgg cctgctgagc aacaccacct acggcagcca
       61 gaagattccc accctgttgg gaaatgacgg caccacagcc ggcaattaca ccaacgatca
      121 cccggtgggc gtgaatgcca ccatcagcag cggcagcggc ttggtttggg ccaacaacag
      181 cttcaccgtg acccccggca cgcccttcgc ccagttcgtc gccaactacg gttggcccgc
      241 tttggcgcct ggcaaatggt cgagcccttg gggagtcaac ggcgcaggtc agccctacgt
      301 gctctgcacc acctgccaca accagcacgt gatgacggtt tacacttcct cgaagaccag
      361 ccagatcgtg ggcgacggtg gcggcaagta ctatgctacc tacttcttcg tgaacggccc
      421 ctacaacccg aacctgcagg gcaagctcac tgccggtcag gcagcctcga cgactcagtt
      481 ctgccgtcag tgccacttcg gcgaagccaa cgaagccaac aacaccaaca acatcccgac
      541 tgtgttccag taaacgtcgg atggcagtac cggggggaaa gggactcctt tccccccttt
      601 tttttggacg aaatatagcg aggaactatg aagtaccaac tcataaccgc actcttactc
      661 acggcaagcg tcgttgcacc ggcccaggtc gcctcgcacg cgcccagcac ggctgccaaa
      721 ctgcctccgg ctcaggttgc tccgctgacc ccgactggca agccagtagc taaggtcaac
      781 ggcacggtgc tcaccgacaa ggaccttctg cgcgagatgt acgccatctt cccctacgcc
      841 aagcagcaca atggttttcc caaggcgcag gaggcgggaa tccgccaggg cgcattggag
      901 atgatcattt tcgaggaact ggtttaccag gaagccctca ggcgcaaaat gacaattcct
      961 gccgccaaga tcgatcaggg cgaggccgcc tacaaaaaga cgttcgacag tccggaacag
     1021 taccagcagt tcatgcaggt cgagatgcac ggctcgcgtc agcagctgcg cgaccagatc
     1081 cagcgttcgc tgctcatcga ccagttgctg aagcaggaag tggacaataa ggcgatcgtg
     1141 tcccaggcgg aagtgaaggc ctactacgac aagaattcgg ctcgcttcca attgccggag
     1201 tcgtacacct tccaggccat ctccattttg ccgccacaaa ctgcaacccc cgcgcaggct
     1261 aaggaagcca ggaagcgggc cgacgaggcc ctgaagcaag ccaaggccac tgccagctac
     1321 caggagtttg gtctgctggc ggagaagatt tccgaagacg acttccgggt caacatgggc
     1381 gaccataaga tcgtcccggc cgataagctg ccgccccagg tgatcaaggc gtttcaagcc
     1441 gtgaaaccgg gacaggtcag cggcctcatt cagatcgaaa atgcctacac gatcgtgcgc
     1501 ctcaacgccc atacgccagc tcacaagcag agcctgacgg aagtgcaggc gcagttaaag
     1561 accgagttgc aaaagcagaa gttcgagcaa ctgcgttcaa acttcgccaa gcagttgcgc
     1621 gccaaggcga agatcgaaat ggcatagtcc actactgagt tttgcggggc gcggcgatgg
     1681 ctgcgcctcg tttcttttgt ggcggccttg ggcctttcgg cacggcattt gagtgatatg
     1741 ccccacagcc tggtgtacag cgcggcctat cccaatgcgg ttaccgacaa cccatttgta
     1801 atcagatgat tacgatatcc caataactct caactatggc acgggtcgtg ctctaaccaa
     1861 ggcaggacac ggactggggt aggccggatc ccagccacgg cgaaaaagag ccatgtcagt
     1921 gatgaagtcg gtgagaactc tcggatggat agtggtattg gccctggcgc catggacgca
     1981 cgcccaagtg cagatcggcg acaacaccca cgtcaacgca ggagggctgt tcaccgtcgg
     2041 gtatgctggc gactacggca accagatcca atccagccac gggctgcagt tcggagccag
     2101 cggcaccatg agcggctatt actacaatcc caacttcgtg aatttcaacg tcacgcccta
     2161 ctataaccag tcgaaagccg attcggacta ccagtcgctc accaatgcca gcggggttgc
     2221 ggccaccgcg aacttcttta ccggcagcca ttttcccggg tccgttacct accgttacga
     2281 ctacaacagc accggcacct tgggactgtc aggcgtaccg aacttcacca cccagggaaa
     2341 cggccaggga ttcgggatca attggagcgc tttgcttccc gacctgccaa ccttgacagt
     2401 cggctaccag caaggcaacg gcagcagcac cctctacggg accaaccagg agagttcaag
     2461 cgaccagcgt ctgtttaatg tgcgctcttc ttatcgcctt gagggattca acatcaatgc
     2521 gttctatgac cacaccactt tgaattccgt gtatccggaa tttcttaccg gtgtcggcca
     2581 ggaaataaac gacagctctg gacaggactt tggcgtcaac acctcacgca acctggcttg
     2641 gatgaatggt tcgatgtacg ccagctatag ccattccagc tattcgacgg attatggtac
     2701 cagtggacaa agctcgacca attctggcta taccgccgat ctcgaatccg caggggtgaa
     2761 cttccatccc acccagaaat tcagtttcta cgccaacgag aacttcacca acaatctctc
     2821 cgcatacttc aatccggcct tggtcagcgg tggagtcgtg actccgcctg tcaacctgct
     2881 gggtagcggc tcagattcgt tgaccgcggg cggaggcgcc aactaccagt ttacgggcca
     2941 tctcaatggc aatgcccaag ccacgtatta cagccaatct tacttcggac agacctacac
     3001 cggaacctac ctcagcggca atgtgaatta tgggcgcaaa ctactcaaca tgttcacctt
     3061 ctccgcgggg gtcgtagaca gcagcaccgg attaggcaac aacaacatcg gattcattgg
     3121 cacagtgaat tatttccgca gattcggagg gtgggagact tcggggagct ttaactacgc
     3181 ccagaatgtg cagtcgctgt tgatcaccga aaccacctcc tactacaact acaacgccaa
     3241 tatccaccgc aaattcagtt cgcgggtgca gtggacggca gccttcaacg gaagccggca
     3301 gggcctgagc agcaataaga attcgtcaag tagcagcgaa ggcttcgcca ccagcctgag
     3361 cttgcgccag gttgccctaa gtgcgcagta cctgacaggg acgggaaatt ccatcctgac
     3421 cagcaacgga cttactccgc tgcccccact ccccggtgtg acgccggaaa atgtgatcgc
     3481 ttacaacgcc aagagctacg gcgggagcgt atcctttacc cctgttcgtc ggatggtctt
     3541 gtcggggacc tacagccgct cgctgagcga caccctgtcc aacggtatct tctccaagaa
     3601 caacaccgaa ataatctaca gccagttgca gtatcgcctg cgccgcatca gtctgctggc
     3661 aggctactca cgattcaccc agggcatcag cgccaccgga gtgccgccgg ggacagtaac
     3721 atcgtattat ggcggtatct cgagatggtt cgatttcttc taacccggcg aggcatgcga
     3781 attcagcgag ttgtgatgtg gatgggcctg gcgctgctgc tgttgtctcc ggccttggcc
     3841 tccaagcagc ccaagcaacc cctcaatgct cctgaaggcc cggagttgct cctcgacggc
     3901 ggccgcaagc tgacctggga acgcagcttc agcctggagc gcgaggtcaa gcccaagcgc
     3961 agtttctgga ccaagctggt tgacgtaatt gccggcgagc ccgacttccg ttacctggtg
     4021 aatccctata gcgtcaccac cgactcacgc ggtcgcatta tcgtgagcga tcccggcgcc
     4081 aacggtgtcc acatcttcga cttcaaccag caaaaatata agttcctcac ccgcaaggaa
     4141 aaggacaagg acggcacgct gacaccgcag tgcgtggccg tcgatgccca ggacaacatt
     4201 tacgtcaccg attcgcaggc cggcaaaatc ttcgtcttcg acgccaacgg caaatatcag
     4261 cacgccatcg gaagcctgaa aggtggcgaa ggtttcttca agcgtcccac cggaatcgcc
     4321 gtcgactccg cagcccagcg catttacgtg accgacacgc tacggaacaa gatcttcgtc
     4381 atggacatgc agggcagcgt gctgcagacc atcgggaagg aaggcacggg cgatggggag
     4441 ttcaactatc ccaccgagtt gcgactgaat ggcaacgacc tggtggtcgt cgatgccatg
     4501 aacttccgcg tgcaggtgct cgaccgttcg ggcagcttcc gttattccat tggcaagctg
     4561 ggagacggca ccggttggat gttccgcccc aaaggaatcg gcttcgattc ggaaggccat
     4621 ctgtacgtcg tggacggagc ctggggcgtg gtgcaagtgt tcaatcagga aggacaactg
     4681 ctctactact tcggccagaa aggcagtggg ccaggcgagt ttcaactccc ggctgggttg
     4741 cagatcgata acagcgacca catctacgtc gtggactcct acaaccgtcg cgtacaggta
     4801 tttcactacc agggcctacc caggcaggct gcgggaggca agccgtgaag ttcctccttc
     4861 gggtgtccag ccttgcgctg ctgtctctgg gaatcgcgtt ctcgcagcag ccgccgtcgg
     4921 atgtgctagg catgcacaat ctgtcgccgg gcagcggagc gtcgatctac gttccctcgg
     4981 gcaatctcgg atgtcagtat tgtcacgcac cccacagtgg cctgggcggc gtaactccgc
     5041 tgtggaacca gcagctctcc aaatcgagct ataccccata cgtgagcacg acctaccatc
     5101 aggtgggcaa cactcagccg ccgctgggcg tcaccagcag cctctgtctc agttgccacg
     5161 acggaaccgt cgcagtcggc cagactgccg cctacggcaa ggtcacgacc acaggcagtt
     5221 gggcgccggg cgactccttt ggaaatgacc tgaccagttc gcatcctttc agcctggtga
     5281 agccgatcaa ggacaccgcc gacctggcag cttcgctggt gtcgcaaggc acgacggccg
     5341 atccgctgca caaggtgaag ctgattaatg gcaacgtgga atgcaccagt tgccacgatc
     5401 cccacaccca aggcattgac cagatcgcgc agaactttct cgtacgcgac agttcgagcg
     5461 ggcagatgtg cctggcctgc cacgacccca accgcgtagt tcaagggcaa atcaatcccc
     5521 tgtcgagttg gccgagcagt atccaccagg cagccgctaa caaagtgtcc acttcagccc
     5581 acgtcggttc gtacgtcacc gtcgcgcaga attcctgcag ttcgtgccac gtcgaacaca
     5641 atgccaacgg gccggcgcga ctgttgcgcc aggcgactcc tcccgccccc ggggtcgatg
     5701 cctccaccca ggattgcatg acctgccaca gcggcggaac ctacgtgtcg cccgcagccc
     5761 ctaacattac cgcagagatg gcgaagaccg gacatcccct gccgtcgggc aacaacaacc
     5821 atgacgcagc cgagcccgct gtgctgatca acaaccgcca tgccacttgc gtggactgcc
     5881 acagcgccca cggttccagc caggtcgtta ccttcggagc tccgccgacc atgcgcgttt
     5941 cgcagtccgg cgtgcaggga atcagcgcgc tcgatggcat caccgttctc accccggcgg
     6001 tgaatcagta cgagaattgc ctgcgctgcc acggcacggg agcgggcaag cagcggcgca
     6061 tcatgtacgg ctacgcgcct gcgcggctgg tgaccatgcc tgacttcctc aacgtcatca
     6121 atgagttcag tgccaccgcg agttcgagcc atcccgtgac ccacgaccgc agcagcccgc
     6181 tgccgcagcc cagcctgcgc atcaacatgc tgaccttgag tggccagcct tcggctcgcg
     6241 ccaccgggcc gcgcatcttc tgcaccgact gccataacag cgatgacaac cgcgaatttg
     6301 gcggcagcgg tcccaacggt ccccacggat cggccaattc ccacatcctg gaacggaact
     6361 atcagttcag ccaggcttcg attccgggag cgctggtcac caacactttc cccaatcccg
     6421 acctcagcgt caacggcccg tatgccatgt gcgccaagtg ccacgatcta acgcaggtac
     6481 tggccaacac cagttgggtc aagcacaaca gtcatgtttc gcaggacgga tttacctgtt
     6541 ccgtctgcca caccgcgcac ggcatgggtg ccaactctcc caacgtcact ggagagcgcc
     6601 tggtgaactt cgatgtcaat gtagtcgggt ccaataacgg gctgccgatt tcctacaatc
     6661 gcggcaccgg caactgcacc ctgacctgtc atcaagttgc gcacaacggc gacggaacca
     6721 cctcaagcgc cttgaaggtc gggccgggcc ggctcaagag aaattaatcg tgacccggcc
     6781 gcaattcgga accgtgccgc aacttttcgc actgttcgtt ctgttcgcgg ccgccggatt
     6841 ccacgaatca atccgccagg cttgcttaaa cagcatcact aatggcgatt tctggtggca
     6901 cctgcggacc ggcctgggga tcctcgcaag ccaagctgtg ccgcacagcg gtgtgtactc
     6961 gcaatctgca tcgctgccct ggacggcgtc ctcgtggctt tatgatgtgc tggtcgccgt
     7021 gggatttagg ctgctcgacc tgcgcacccc tgctgttctc gccatagttt tcaagttcgc
     7081 gctcgccctg ctgacgtttc ttctggccgg cggattgcgc ggaaaattct ggaccgccgt
     7141 agcgctctcg actgccgcgc agtacatcct cggcgccgtg ccaccgcttc ccctgtattg
     7201 ttcggtgctg gcgtttgccg tcgaactcct cctgctcatg caatgtcgcg cgaccggaaa
     7261 tgcccgcccg ctttattggc tgccgctgct gtttctgttg tgggccaacc tcgacgtgca
     7321 cttcgtgctg ggaatcgtct tgttgctgct gttcgtggcg gcctgcgcca tcgagcagtg
     7381 gggaacacgc gcgcgcatgg tgtggctggt gcggtcatca cgtccgccgt tgaacgcgct
     7441 cgcggcggtg accttggcgt cgctcgtggc cacggtgatc acgccctacg gatggaacct
     7501 ctacggtctg tttctcgctc gcgtgaccag cgccgctaac atttatttcc ccggcttcca
     7561 atctttgcgg tttcgcagtc cgcaagacta cctgctcctg ctcctggtca tggccgcgtt
     7621 tctcgcgctg ggaatgcgcc gttcgcgcga cttgttccag atcgcgttgc tgctgctttg
     7681 taccatggct gcattccacg cgcaacgcga cgcctggctg gtaacgctgg ctgccgtggc
     7741 ggtcgtgggc aacgcggttc cgcaaaccaa tttgcaatcc ggccgtgagg acgcacgaat
     7801 tccggcacgc cagtttttct cagctgcggc cttggcgctg gtgctgcttg ccatcgcgac
     7861 ggcgatccat cttcctcacg gtcgccaagg cctgctggcc gagatcggcg aaggctatcc
     7921 cgttgccgca gccgattaca tccgcgagca ccaactcccc cagccgctct tcaactcgtt
     7981 cccctgggga ggcttcctca cctggtatct ccctgagtat ccagtggcca tcgacgggcg
     8041 caccgatctt tacggcgatg acttcaacat ccagtacgcc aaggtcatga acgccgaggc
     8101 gcattactcg acgtttccgc cgctcgcccg ggccggcacc atcctgctgg agaagaaatc
     8161 gctgatgggc tcggcgcttc agcacgttgc gggattcaat caggtgtatg ccgacgacgt
     8221 ggccgtcgtg ctgctgcggg agcagcccca gccatgagcg ccactgcgac cgtcaccgca
     8281 gttccgcaac ccaccgctcc ccctaaatgg gagtcgatcg gcaattacat ggtcctgctg
     8341 gcgtattccg cagtcgtgtt gttcaccgtc cgctaccacg aaaaatgggc ggacgaagcc
     8401 caagcctggc tcatcgctcg cgaccttgat ctcaagacca tctggtttca cgagctccgt
     8461 tacgaaggcc accctggcct gtggcacacc atcctctgga tagcacagca cgtatttcac
     8521 gcgccttacg cggcactcgg ttatatcggt gttgcctttg ccatcgctgg agttgcggtg
     8581 ctcgtgctca aagcgccgtt tccatggttc atacgctggc cactcgcatt cacttacgtc
     8641 ctcgtctatc agtatgcggt aatcgcgcga tcttacacca tactgccgct gctttgcttt
     8701 ctcgtcgcga ttttgttcaa ggacatcgag catccggaac gaatgacgat tgtgctggtg
     8761 ctgttgacca tgctgacctt gcacggcgca attctggccg cgtgctatgg gcttctgtac
     8821 ctggtggaag ccatcaagtc ctggccccaa ctgaacgttc ggctgcggga ccgctatttc
     8881 atctgcgcag ccgtgctgac gtttgcgttt ctgtttctcg tcgtcatcct caagccaacg
     8941 ccggacgtcg gcgaatttgc tacgaagagg gccctcgatg ccgccccgga ggcggtcaag
     9001 gccacatttc caactgcgtt ggcgaaactg acagcggtaa tttcgggtgc ctttctggat
     9061 ttttggctat cctccacgat ttttctggtg ctcgctgggg catggtgtta cacgcgaaga
     9121 aggctggtgc tttacatcct tcccatggga ttgctgattg ctctttatgc caaggtgcac
     9181 ggctatccgc accaccacgg tacggttttt atcgcggcca ttacggcgtt ctggatcgcg
     9241 tggccatccg aagacgaaac ccgttcgttc aacgtccgtc agcatcgggc gatgcatgga
     9301 atgatcgcgt tattgttgtg cctgtttgcg gtcaatatct gggacgcggc cgtcgtcatt
     9361 caacgcgaat atcgctatcc ctatagcggt tcgaaagatg cggcaatgta tctgaagtcg
     9421 gttggcgccg atcgcggcaa ggtgttcggt ttcttgtttg gcgtgaacgc tgtgcaagcg
     9481 tactttgacc acaatattct ggcgaatatt ccgaccgcgt actatcacaa cgggctgccg
     9541 cttgtagggg acacgctgaa cgtagccgaa ctaaatcgcg tgaggcccga gtacatcgtg
     9601 gcgtattcgg gagaaccaca gctgatgatt agggttggga ttccagaact ggagtcgcga
     9661 ggatacaaga tggtgcactt ctctgacggc tactacatgt acaagcaggc cgtttaccag
     9721 cgcgagtcgt attttattct ccgccgcgtc cagccctaaa cccgtccaaa attctgtttc
     9781 gctataccgg tgaatgttgc aggcgtccgc cccaactctc gtggttactt tagtttcttc
     9841 gccccgtcga ttgtgcgtga cttccacttc cgctggggtg ctactggcgc ccaaatacca
     9901 cggccaaaga atcctggtaa atcagcttga accggggatc ggcggtcaga atcttggcca
     9961 gtggcaggtc cgcagccagc accaccacgc ccgcctcatt caggtaagga tcggtcgtgt
    10021 atccggcctc ggcgctttgc gatgcgtaga agagttggtc gagttcgtcg ccgtagaggt
    10081 cgttgcggcc atcaatggcc acggggtagt cgggcatgta ccacatcagg aatccgcccc
    10141 accccaggtt gttgtacagc gggccgggct gcggattgcg gcgcagaaaa ttgaccgcgt
    10201 tgaccgggta ctcggaactg atggcgcgat ccagatcacg tgacgtaaag tccgtgccgc
    10261 gggatatgac cagcagcagc accaccaccg cagccgccac gccgaggttc tccatccagt
    10321 tctccgccgc atcgcactcg gcttccgcag cagggaagtc ggcaatacag gcggccgcgg
    10381 gaatggcgac gaaccacgca tcgcgcatgg tgcggtaggc caccaggctg caagcggtca
    10441 tcatggccag cttgaacagg tcgatcttct tgcgccaccc caccgcaaaa aatccgcagg
    10501 cggtcagcag cagttgcacg tagtgactgg atccgcggaa gctgagcggc tgtagctcga
    10561 tgatcacgtt gtacgcgaac ttggccttcg aatactcgta caccgcgatg tagggatggt
    10621 agaaattcgg cccaatcagt gtggccagca agcacgaggc aaaaacgccc agcagaggca
    10681 aggagggcag ggttgcaggc accagaaagc tcggcgcgat cccccacgag tcggcgatgc
    10741 gctgcgcgag ggtggttccc accagcagcc cgatcaggaa cagtccgtag atgaactgga
    10801 tgtgcaggtt cccccacacc cagaagatca gcggcagcca gtacaacaat tcgacccggc
    10861 ccgtgcggtt ggcttcgagc agcaacgcaa gtgtcacgca gaacagggcg atggagaaga
    10921 acacgggacg cggcattccg ttgaacagga aagcccagca ggtcagcgct gccagcacca
    10981 gcgcgaccca gaagcgcccc gagatgcgac gcagcatcca gtaaaccgca taggcgacga
    11041 gaatagtgat gagggtcccg tacaatccga tgcccatcag cccgaaccat ttgtagacca
    11101 atgacatcag gacttcgtag ccccaactgt aggccaccca gggacgggtc gccgcggtgc
    11161 gcgacaggat gccggtgtgg ggaacggcga ggtgttcgac catccagtca ccgactttca
    11221 ggtgccacca gatgtcgtta tcgatgacgc aaagcttggt gtagaagctc atgccgccga
    11281 tggcggcgag gaaggcggcg aggcccagca gttgcagcgg ccggaagcgc agcagggaac
    11341 gcaggtcctg ctggccgctc atcgttcagt acccgggcat gggaacatag ttgcgcagtg
    11401 tcgaaactgt taatttggcg tgcagcagca cgcctgcatc atcgtgattc cggagccagc
    11461 gaggcttcct gtggtttctc cggcaccagt ctgagcccgc tggagtgctc acccgcgctg
    11521 ccagcctcgt ggtattcgcc atcctggttg acggcccaca catcgtacag ctcgcggccc
    11581 gcagcgatgt tcttggcagc cagcattgcg gtcatcatgg catggtcctg gttgttgtac
    11641 ttgtgcatgc cgttgcggcc cactagatgc aagttgatgc agtgttccgc cagggcctgc
    11701 cgtatggtcg agacgtgagt ctgatacgcg tcgtcataca ccgggtatgc cttcttttgg
    11761 cgaatgacac agccatccac cacgtcttct gccgatccca atccgatctg ctggatttcc
    11821 ttcttcgcca tcgccagcaa cgtattatcg gacgcagtcc acagaccatc gccttcgaag
    11881 caaaagtact ccaggccgta acagcagaag gccgggtcgg gaaccatctc cggcgaccac
    11941 gatttgtagt tctgcacccg gcccacttgc accgaagaat cgtggatgta gatccagttg
    12001 tcagtgaagc ggtctttatc gcgcaggatg agcccgatcg tcagaaaatc gcgatagcgc
    12061 aacgaccgcg ctgcgttgag tgcctccggt ggaagctgtg gttcgagctg cgcaatcaac
    12121 tcctggatag gcatcgagga aacgaggtgc tcggcctcaa actgttcgat tgcgcccacg
    12181 gcagttcggg cgctgatggt ccaatgtttt gtctgcgagt cgaaacggca gccgacgacc
    12241 ttgcggccaa gcagaacttc accacccagc gcgcggactt tgttcgcgca cgattcccac
    12301 atcattcccg gtccctgccg ggggtatcga aaggtatcga tcagggtctt tacgacgttt
    12361 ttgcggtcct tggattttcg ctgcggcagt atcgcatgct tgatggccga tccgagcgag
    12421 agccccttga tgcgctgcgc cgcccaatcg gccgagatct cactgcaact catgccccac
    12481 actttttcgg tataggtctt gaaaaagatc tggaacaatc gctctccgaa ctgattgact
    12541 acccagtcct cgaaggtctt gggattcgga gtgggatgaa ggcgcgccgc agcaaacgag
    12601 gccatgcagc ggaacgattc gaacagtccc aacttcgaca gcgcttcaaa cggcttcaaa
    12661 gggtagctgt aaaactgccc gcgatagaaa atgcgcgacg accgcggtct ctccagcatg
    12721 aggtcgccgg caatttctgt ccaaagatct tcgacctcgc gagacttgga gaaaaagcgg
    12781 tggccaccaa tatcgaagtg aaaccccttg tatctaacgg tgcgggagat gcctccgaca
    12841 tactcgggat cgctctccaa caccacgacc ggctgtttca gcttggagag ctcgtaagcc
    12901 gcagtcagcc ctgccgggcc ggctccgacg atgaccgact tgtatacttt tcgatccatg
    12961 gcgaataacc gtaagtatcc gtgagagcac ctgcggttcc aaaccttatc acttgtaact
    13021 cattgaggcc aaaacacaac tcattccccg agttgcggag gtacagagca tgaatgggtc
    13081 aaaaggcgcg tggagtgagg ctaaaaacgc gatgccagga aaataagcgc cagatccgca
    13141 atgaggatac agctaagtga ttgtatacaa acattatgtc taccatgcgc ttccgctgga
    13201 gtttggcagt acgggtgcgt atacatacat ggttcttctg cgcggagtgg ccccttgcgt
    13261 ctcgagaacg acgttttgag gagttgctga atggataacg aagataatca gcccagaacc
    13321 gcgggcacca actggacgcc gaccgcaacc tacgccatgg cagcagtctg ccttctgatc
    13381 ggcgtactcg tgggatacgt cgtgcgcgga tcggcacccg ccgcaagcca tccccaaatg
    13441 gcggccagcg aaggtgcagc tccggcaaca gcgccagcga tgccagcggg catgacgggt
    13501 gacgcccctc atcccgcccc aaccctggat gacatgaagc gtatggctga taagaaggcc
    13561 gaaccgctac tggccaaact gaagaccgac cccaataacg ccgagacact taacgagttg
    13621 ggcatcctat accgggccac tcaccagttc aaggaagccg aaggctacta cgaaaaatct
    13681 ctgcaaatcg atcccaagaa cgtcaaagtt cgtaccgacc tggcttcgtg cctgtattac
    13741 accggagacg tcgacggcgc cctggcgcaa ctgaacaaat cgttgaccta cgatcccaag
    13801 cacgctggaa cgttaatgaa tataggcatc atcgagtggc aaggtaagaa agatgtcaac
    13861 gccgcagtag ctgcgtggca gaagctcctg aaactcaatc cggacttccc gcaaaaagat
    13921 gtcgtcgaga agatgatcgc agaggccaag ggacagggaa ctacggcgca agccgaaagt
    13981 caaactaaat aaactctgag tgaccagacg aacgcgaagc ggaccacgat actagctcgg
    14041 gttcgttgtc ttcgctgatt cttcctccag caattccaga acgccccggc gtgcggcctc
    14101 gttttcagac ccgaacgtcg gtggctcgat gtcttcataa aaccgccatg cgctatgcgc
    14161 gggaagggaa ttgccctccg cgtatgcctc gcctgccact tcacacagta aagcgaacgc
    14221 caggctttga ctcatgcgat gcagcagttt cctggcaaag cgtggtgcgc tctgttcgtc
    14281 gcgcaacgtg atcgccagcg cgtcgctgtc gccttgtaat cgttgcgcca acgcttgacg
    14341 caaattcccc agcccgtccg gagtgcggtc tagaatttcg tgcgctcgtt ttcgatatgt
    14401 ttcccatccc tgatagcgtg gcgccatcag gcgtagcaac tccagcgcct ggatattagc
    14461 cggtccctcc caaacagtta gcacttgggc atcgcgcagc aatcgcgcca caggataatc
    14521 gctggtgtat ccgttgccgc cgatcaactc cagcgcagcg cgcgccgaag tgatggcata
    14581 ctcggccgtg agatatttcg ctaatgcagt caccagtcgc aaccaggttc ggcgctcggt
    14641 gttctgctgc acctcgtcga acgtgatggc agcctcgaat gccagcagcg ttccagcctc
    14701 gaactgcact cgcatgcgca acagttcgta ctgcaccatc ggataatccg aaagtgggtg
    14761 cccgaaagcc tgccgtgtct ttgcccatgc gagcgcctct cgcaacgccc tgcgttctac
    14821 accgacggat cctacggcgt tatgcacgcg gctatattcc agcgcctcca tcattaattt
    14881 gaaacccaag ggtggagcgg cgatttctac cgcttcagct ccaagtaact cgatctcgcc
    14941 agtcgggagc cccttggtcc ccagcttgtc ttttagcttt cggatgcgaa agtggttcgg
    15001 tcgaccatct tcaagatgac ttggcaccag gtagagtccc aatcccgatg agcccggcgg
    15061 cgcaccttct ggacgtgctg tcgctacagc aagcccgcta ttggcgttgc tggcaaacca
    15121 tttcaggccg tgcagcgcaa agtggtctcc tgcaggcacg gctaccgtag tggtcgctcc
    15181 tacatcgctt ccgccgtgtt gttcggtggc ccacgtgccg ccggtcttgg cgtggccatc
    15241 catgcgaacg acatcgtaga gatagcgctg tcgcacatcg tcgggggcgc gcgatcccag
    15301 cacatacgca accgcgcccg ttagcgtcac tgggcaatgc aagctgatgt cagcttgcgc
    15361 gagcacgtag cccattgcga acgtcagcac atgcggcgcg tcctcgacat agggcaaacc
    15421 gatgatgccg cggcggtaaa gctcctgatg ttgagtctcg taccatggat tggcgacgat
    15481 gcgatttacg acctcgccat tgcggtcgta ggtttccagc gacggtggcg cgtaacggtc
    15541 ggtgtagttc gcctgcgcgt caacttcctt aagcacccaa tcgcggaagt cggcgagttg
    15601 cgcactgtag tgcgccatcc gcggtacgcg gacgttaagc caaggttcga gatacaggtc
    15661 gaagctgcga gagtcggtca tgatcgcctc aggagaggcc gcgaagcacc aagcttacag
    15721 gtaatgcatg tggatggaaa gaatggtagg tgcacgagga gactcgaact cctgacctcc
    15781 accgtgtcaa ttcctggccc gatgagaagc ctgacgatac cgagagagat acagcatacg
    15841 cagagtcagt gttcatgcgg ctttggtcgc tctgcattgc cactgactta tccccgagcg
    15901 acacctaccg acacaggcag agttggacag caatggtagg ggtaggacac aagtgacgac
    15961 acatcgttgg gcaaacgcca gcatagagac aatctgcaag gcctgagctg acgcacgcct
    16021 taccacagta cattgctatg caggaacgga cgcgccgtca gtcgccatgt gttgccaacc
    16081 accgacgcgc tggataagag catggcgacg gcggccatga tcgggttgag cactccggtg
    16141 attgccagcc cgatgcccag cgcgttgtag aagaaggccc agaacagatt ctgtcgaatc
    16201 actcgcatgg tcttgcacga gaggtccagg atggcgggaa tcttctccag ggttccgttc
    16261 atcagcacaa cggctgcaga ggaaggtgac cgggcgcagc ttcatcagcg aattggtgct
    16321 gtcgcccatg tcgcgaatct gttccttgaa gcggcgcgag gaggtcgcag ttcccagttg
    16381 accgttgccg ttgatgtaca acgcgatccc acccgccgat gtcacgccat ggataccgcc
    16441 gatgaaggtt tgcgttgctg aactccctgc cgttccaatg cggatggtgt tgccctcgtc
    16501 acagggctgg acacttgccc cactgtaaat attgccactg cccgtcacgg gtgctaaggc
    16561 cggccgaatc gccgatgaaa gtgttgctgc ttcccgtcgc cgtggtgtta ccgcccgctt
    16621 tgtagccgac gtaggtattg tggcttcccg tgctggccag gccagcgagc gtgccgatgt
    16681 agatatcttc gcttcccgac ccagtattaa ggccagattg gtcgccgatg aagatgtcgt
    16741 gtatttgttt agcggggagc gggcggggtc aagtccaagg cttggggtgt gcgcagatgg
    16801 agcaatcgtt ccaagaatcc ggtggctggt cggagctgct gacggcaggt ctgtaggata
    16861 c
//
LOCUS       DASRKK010000199         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_600489, whole genome shotgun sequence.
ACCESSION   DASRKK010000199 DASRKK010000000
VERSION     DASRKK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600489"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VFC15_04390"
     CDS             <1..1331
                     /locus_tag="VFC15_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09430.1"
                     /translation="ISTPYWHALELLDQRRGILVPFNDPDAIAEQTIQLLENEAERHA
                     MRKRAYLYSRNMVWKSVAQDYMRSFQRARAERMWNPRLVSPNAVGADRVHGLPPLKLN
                     HLSRMTDDTGMLQHAVFAVPNHHEGYATDDNARALIVSILVEQLGADRELEAKKLASR
                     YLAFLWLAFNSANGRFRNFLSYERQWQEPQGSEDSHGRALWSLGTAVNQTIDEGLRGL
                     AGRLFELAVPAVLAFTSPRAWAFSLFGIQAYLDRFPGDRSAQRQRDALADKLLNIYRI
                     TRTPGWNWFEDSLAYSNARLSQALLLAGSRTSDKELLTAGLESLEWLVMQQCCNVNGH
                     FVPIGSQGFYCKGGGKARFDQQPVEASATVSACLEAYRATGDERWRKEAWTAFNWFLG
                     DNDLKTALYDATTGGCRDGLHPDRANENQGAESTLSFLMALLEMRQLQDV"
     gene            1801..>2280
                     /locus_tag="VFC15_04395"
     CDS             1801..>2280
                     /locus_tag="VFC15_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09431.1"
                     /translation="MTLATDAIDATDATDATDSTDFLLHRHRANPILSSKDWLYRINS
                     VFNAGATLLADGSTLLLCRVEDRRGLSHLCAARSVNGVNGWDVDPKPTLMPNPNEYPE
                     ELWGIEDPRITYVPELEQYAVAYTAYSKGGPGVSLALTKDFRTFERYGFLMSPDDKDA
                     "
BASE COUNT          486 a          630 c          656 g          508 t
ORIGIN      
        1 tcatctcgac gccctattgg catgcgcttg agcttctcga tcagcgccgc ggaatactgg
       61 tcccattcaa cgacccggac gcaattgcag aacagaccat tcagctcctg gaaaatgagg
      121 ccgaacggca cgctatgcgc aaacgggcgt acttatattc ccgcaacatg gtttggaaaa
      181 gcgtggccca ggactacatg cgcagcttcc agcgggcccg agcggagcgc atgtggaacc
      241 ctcgcctggt cagcccaaat gcagtgggcg cagatcgcgt ccacggtttg ccgcctttga
      301 agctgaatca tctcagtcga atgactgacg atacgggaat gttgcaacat gccgtgttcg
      361 ccgttccgaa ccatcacgag ggctacgcca cggacgataa tgcccgcgcc ctgatcgtgt
      421 cgattctggt ggagcaactt ggggccgatc gagaacttga ggcgaagaaa ctggcttcgc
      481 gatacctggc tttcctctgg ctggccttta actccgccaa tgggaggttc cgcaattttc
      541 tgagctatga gcgtcagtgg caggagccgc agggatcgga agacagtcac ggccgtgcat
      601 tgtggtcctt gggaaccgcg gtgaatcaga cgatcgatga aggtctaaga ggcctcgctg
      661 ggcgtctgtt tgagcttgca gttccggcgg tgctggcttt taccagtccc cgagcttggg
      721 cgttttcctt gtttggaatc caggcatacc tcgatcgctt cccgggagac aggtctgcac
      781 aacggcagag agatgcactc gctgacaagt tattgaatat ctatcgaatc acccgcacgc
      841 cgggttggaa ctggtttgaa gatagtttgg cctattccaa tgcgcgtttg tcgcaggcgt
      901 tattgttggc aggctcgcgc acctcggaca aagagttgct tacggctggc ctggagtcac
      961 tggagtggct tgtgatgcaa caatgctgca acgtgaacgg ccacttcgtc cctatcggct
     1021 cgcagggttt ctactgcaag gggggtggaa aagcccgctt cgatcagcag ccggtagagg
     1081 caagcgcaac agtttccgca tgtctcgagg cgtatcgcgc caccggagat gagcgttggc
     1141 gcaaggaggc ctggacggcg ttcaattggt tcctgggaga taacgacctg aaaaccgctt
     1201 tatacgacgc caccactggc ggctgtcgag atggccttca tcctgaccgg gccaacgaga
     1261 accagggagc ggaatcaacg ctgtccttct tgatggctct tctggagatg cggcagctcc
     1321 aggatgttta agcgaaagag atcgcgcttt agatcagttt tcggaatggt gtagtccggc
     1381 gcgggtacgg gtagagcggc gcttcagccg ggggttattc gtctgtttcg gtagccatag
     1441 tagacagcaa gctttgaaag ggcacggctt tcagccgtgc ctttacgtgt gcgtgaggag
     1501 ttgggcttca gcccctgagg gaattcgcac ttcgtctcaa ccccgaagtc cctcagtggc
     1561 taaagccatc ataatgacag cagcttacgg cacggctgaa gccgtgcccc ttcaaagcgt
     1621 gaagtcctca tgaataatca ggttcagcgc cgcgttgcag cgaatcctcc agaaacgagc
     1681 cggcttcagc cggcgacact attgttccag atcggcccta aatggctcca acaggcttgc
     1741 cggcgcgggt cgattgccaa cgtcttggaa tctcagcttc atcttcctgt agaataactt
     1801 atgaccttgg ccaccgacgc tattgacgct accgacgcta ccgacgctac cgactctacc
     1861 gactttctcc ttcatcggca tcgcgccaat ccaatcttgt ccagcaaaga ttggctttat
     1921 cggatcaata gcgtattcaa tgccggagca acgctgcttg cggatggctc cacgctgcta
     1981 ctttgccggg tggaggatcg ccgtggtctc tcccatctgt gcgccgcgcg ttctgtgaac
     2041 ggcgtgaacg gatgggacgt cgatccaaag ccaacgctaa tgcccaatcc aaatgaatac
     2101 ccagaagagc tttggggtat cgaagatccg cgtatcacat acgttccaga gctggagcaa
     2161 tatgcagtgg cctacacggc atactcgaaa ggcggtcccg gagtttctct cgctctaact
     2221 aaagatttcc ggacgtttga gcgatacggg ttcctcatgt cgccggacga caaggatgcc
//
LOCUS       DASRKK010000200         8556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_209397, whole genome shotgun sequence.
ACCESSION   DASRKK010000200 DASRKK010000000
VERSION     DASRKK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209397"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFC15_04400"
     CDS             complement(<1..611)
                     /locus_tag="VFC15_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09432.1"
                     /translation="MATTIKATRQVVEDYQRLFVNRRAYTMQSMRPHPETGRHYYFRP
                     SKKGTDVPLTLTDETIRQHLEGKITVGLYAINPATQRCKWVAIDADYKNSVEDLLKLQ
                     YHLTQDRVEPALEMSRRGGHLWIFLATPLLAKECRIYIHNLALRLGVPVKGAGLVDGI
                     EVFPKHDAIGTGEFGNAIRGPLGIHRGANRRFWFYGADYTLED"
     gene            complement(723..1100)
                     /locus_tag="VFC15_04405"
     CDS             complement(723..1100)
                     /locus_tag="VFC15_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09433.1"
                     /translation="MMVEPVVQLDPGIDLQTAIATLLKAAENVEIVRWIELPASVLLF
                     LLAPGDPGSGAVYVLDRKKGTWYAVDFEDEQFGGYSVSQLETLLKDCNFLDLIERPGL
                     WRSGLRWVVLPGMRPETAVETAV"
     gene            complement(1637..2749)
                     /locus_tag="VFC15_04410"
     CDS             complement(1637..2749)
                     /locus_tag="VFC15_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09434.1"
                     /translation="MNNLATDPVKHGELLVTVNKITNDLIAAEVTNPRWQMVFSTSKR
                     TLRAFDVDASRRRRRVSAIAAALLVVIPAPAMAAEMNCNTTNTGSSATTNTVVADDLS
                     VHFFGNTTHLQATSDQVGLATQTGPLPISVSGNVQSWTSGPPLGDLYTESAQGFYQLN
                     QKWKILGQQLYQQQGSISLSNFVGGFNYRPNNDLSLNVTVGAGVHTLYTYQWSAYFSP
                     QYRLPWTVCGKKPVALELDTTFENYVLGNFSQATPKLDLDLTSWLPQFQIGYAFGDFD
                     STTSVNPTQYYQPQAIRGVTFTAVAHPLDQMYVVVSYLPANRNYIAGSYVTQNTFGGT
                     IHWNLAAGFRLSFYAEDSWYKGGSDRALGGGVSFAF"
     gene            complement(3109..3315)
                     /locus_tag="VFC15_04415"
     CDS             complement(3109..3315)
                     /locus_tag="VFC15_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09435.1"
                     /translation="MEAPSDKRWRTYTNTKFDPRRTPEYIVKLLESIQVDHENSEGAV
                     PRHRIPDFLLPTPLKHESIEEAGE"
     gene            4112..5827
                     /locus_tag="VFC15_04420"
     CDS             4112..5827
                     /locus_tag="VFC15_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZM09436.1"
                     /translation="MKRAFIDHGLSLLAGGILMTGPVVAVAQALQATPPRTDATGKPP
                     YNIILVIVDQRSYRLFAGSGYSLPAINTLARHGVTFRNHYTASAMCSPSRAALLTGQP
                     PQVNGVLDQMEYSFVSSLSTNWPNIGSVLKELAYKTAYFGKFEMDKDLLKPNGSVNYS
                     AALLPYGFDQFNAVGDIGSAPESGFDNDAFIAGESVSWLRANAPEARRSGQPFLLVAS
                     FVNPHDVMYANANVPGQPPVQKAVTPRSIPPLPSDATYDRKWSFTFPASLQESLTAPG
                     IPNALLEYQQGWSGWSGIIPTDRKDMWGIFYNYYLNTIRDNDRSLQQVVDAMNDMDLW
                     RDTVIIYTSDHGEMAGAHGGLKGKGPFAYEANVHVPLIIAHPSGKPGSSCSALTSHLD
                     LLPTIFGLTGLPESRRPAAVKALPGHDFSALLANPQPANAQTVRPGVLFNYLGLSTID
                     GDYLRELMSAGQLHQATPPVTQVNLDKRGYLSFVFDGRYKFGRYYAPNAFNTPKTLEE
                     IFKYNDIQLFDLENDPDEMHNLALTPKRNQALILRMNGLLNNLMAKEVGIYDGRTIPQ
                     VVGSK"
     gene            6213..7538
                     /locus_tag="VFC15_04425"
     CDS             6213..7538
                     /locus_tag="VFC15_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09437.1"
                     /translation="MKKQVVISTSLMAVLLLSSAVGFGQQDATSGGDKLAPAMGKNVT
                     STAASYLSFDSIDSDGQYSHVSSGVGFRYIGTYSVKRLNQVLTTELSEFTTFKATYPP
                     ARYPVKLYRIIYHSVIPEQDNRPTIASGLIAIPETGLPAMPLVSYQHGTVFTKTGVPS
                     HPDESMETRLMIAEFAGQGYAVIGADYFGKGLSPEADSYLVKASTQQACLDMLFAARA
                     VGSALKVQFGPLFLSGWSQGGWSTMVFLNKLNSLGISVKAAAIASAPNDLFAILNRWM
                     HTPAAIDADYLPALIALQANAYEEYYQLPGLAASAIKTEYLQTARDLYLNKLTWEQAQ
                     PKLPKRLNDFLQSGFIIASSIGDTRFYRIVQDSQDYRWRSATPLRTYYGDIDEVVPSY
                     IATLPVQYQEIMGGATVTAIDAGNLANHRGTFVYGVADQKKWFDQLIAK"
     gene            complement(8069..>8556)
                     /locus_tag="VFC15_04430"
     CDS             complement(8069..>8556)
                     /locus_tag="VFC15_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09438.1"
                     /translation="HYLFADKFGRPAKGNDKGKVEGLVGYIRRNFLVPIPKVASWEEL
                     NQRLLDMCQTLLSLAGFQVILIGRFWVIAEGCARASCTSWCRDTRGLCEPQWFGLGDE
                     CTRLSFERRGGVLWTDPPAWVGTSVASCCHSRAGCSRSVRMGSCKTSGTVHGSFEIRT
                     V"
BASE COUNT         1851 a         2431 c         2361 g         1913 t
ORIGIN      
        1 tggtcctcca acgtgtaatc ggccccgtaa aaccagaatc gccggttagc gccccggtgg
       61 attcccaggg gtccgcgaat cgcgttgcca aactctccgg tgccgatggc gtcgtgcttc
      121 ggaaacactt cgatgccgtc caccaagccc gcgcctttta ccggcacccc caacctcagg
      181 gcaagattat ggatgtagat tcggcactcc ttggccaaca gaggcgttgc caggaagatc
      241 caaagatgcc caccgcgcct gctcatttcc agcgccggtt ccaccctgtc ctgggtcagg
      301 tggtactgga gcttgagcaa gtcctccacg gagttcttgt agtcggcgtc gatggcgacc
      361 catttacagc gctgggtcgc cgggttgatg gcatacaggc cgaccgtgat cttcccttcc
      421 aggtgttggc ggatggtttc atcggtcaac gtcaggggta cgtcggttcc tttcttgctt
      481 ggccggaagt agtagtgccg ccccgtttcc gggtgcggcc gcatggactg catcgtgtag
      541 gcgcgccgat tcacgaacag cctttggtaa tcctcgacga cctgccgggt tgccttgatt
      601 gtcgttgcca ctaatccgtc acctccttct gtcaatttcg cgaatccggg gctcgtgttc
      661 gccatttccc cacacggttt tcggatgtgg gtgtcgttag ataacccgga atattctttc
      721 tgtcaaacgg ccgtctcaac ggccgtctca ggccgcattc cgggtaatac cacccaccgc
      781 aaaccggagc gccaaagtcc cggccgctca atgagatcga ggaagttaca gtccttcagg
      841 agcgtttcga gctggcttac gctgtacccg ccgaattgtt cgtcctcgaa gtccacggcg
      901 taccaagttc ctttcttgcg atcaagtacg tagaccgccc cagaacccgg gtctccgggc
      961 gccagcaaga acagtaggac cgacgccggc aactcgatcc agcggacgat ctcgacgttt
     1021 tcggcggcct tcagtagtgt ggctatcgct gtctgcagat cgatgccggg gtcgagctgc
     1081 acaaccggtt caaccatcat ttgtccaaac atcgcagttg tcctttccgg gcagtctgcc
     1141 caattcgtgt tgtgttgaaa gccaagacgt gaccgatgac gtacacgtca gtttgcggag
     1201 gaggggaaag tgcgttaagt atcttgtttt gttggcagta ctagggttga gagtggagta
     1261 gagcagaaag aagatcgcta cgcaggacga tctctatata tataaaggat gcaagtcgcg
     1321 cgctcagatc gattactatc cgccgatggc gcggaaaggt gagccaccaa caatgccgct
     1381 ggtactccgg ttcggcatct ccccaagccg gtttgggtcg agtatgaaaa gcgaatggcg
     1441 ctcgaagatt gaaccgcact tagatggcgt agttgtggct gcgcaccgac gacgaagtag
     1501 gcttgagctt acgagcaccg gcgcctgagc gggagcgtat cggctcgccg taactatcaa
     1561 gcttcccgag aacaccgaga acacaaggat tgcaaaaatg cgaggagtcg taggcctacc
     1621 atcaaccaga ctgtggttag aacgcgaaac tcacaccgcc gcctaaggcc cggtcgctgc
     1681 cgcctttgta ccagctatct tctgcataga aggaaagtct gaacccagcc gccaggttcc
     1741 agtggatggt gccgccgaaa gtattctggg tgacgtagct gccggcgatg tagttgcggt
     1801 tggctggcag ataggagacc acgacataca tctgatcgag agggtgcgcc acggccgtga
     1861 atgtgacgcc acggatcgcc tgcggctggt agtactgcgt aggattgacc gacgttgtgc
     1921 tatcaaagtc tccaaaagca tagccgatct ggaactgtgg cagccaggag gtaagatcaa
     1981 gatcaagttt tggagtcgct tgagagaagt taccgaggac gtagttctcg aaggtagtgt
     2041 caagttcgag tgccaccggt ttcttgccgc aaacagtcca aggcaggcgg tattgcggcg
     2101 aaaaataggc gctccactga taggtgtaga gcgtgtggac acctgcgccg accgtcacat
     2161 tcaagcttaa atcgttgttc ggccggtaat tgaagccgcc gacgaaattc gaaagggaaa
     2221 tcgagccctg ctgctggtag agttgctggc caagaatctt ccacttctgg tttagctgat
     2281 agaaaccctg tgcgctctcg gtgtacaaat cgcccagcgg cggtccactg gtccaactct
     2341 gcacattacc ggagacggaa atcggcagag gaccggtttg cgtcgcgagg cctacttggt
     2401 ctgatgtagc ctgtagatgc gttgtatttc cgaagaaatg gaccgacaag tcgtctgcta
     2461 ccaccgtgtt tgttgttgcg gagctgccgg tgtttgtggt gttgcagttc atctctgccg
     2521 ccattgccgg tgccgggatg acgacaagta acgcagccgc gatcgcgctc acccgcctcc
     2581 tgcgacggct tgcatccacg tcaaacgccc tcagcgttcg ctttgaggtg gaaaagacca
     2641 tttgccaccg cggattggta acctcagccg caatgaggtc gttcgttatc ttgttcactg
     2701 tcacaagcag ttccccgtgc ttgaccggat cagtcgcgag attgttcatc tcctccgggt
     2761 ctcggttgag atcatacgct cgagatcatt ctcggggagg atatcgctcc agccatttcg
     2821 cgctctccaa gcgaacgcca gcggcgcacg attgatcctc ttggcagcgc ggatttcatc
     2881 gatctgatgc cgccagaggc tattgagacg gtggcttcgg ccgagaaggc tgcccaagac
     2941 cagtggcact cccctgaccc cgcgcaactc agtcgctgcc cggtcagaac gggtgaggaa
     3001 aacctccgaa ccatggatcg caaacggaac atgaattcgg ggaagctccg gatggacctt
     3061 gagttgtttc tcggcactgc gcgactggaa cagagagccc tgcacggttc attcaccggc
     3121 ctcttcaatc gactcatgtt tgagaggcgt tggcagcagg aagtctggaa tgcgatggcg
     3181 cggaacagca ccttcactgt tttcatgatc gacctggata gattcaagca gcttaacgat
     3241 gtattcgggc gttcggcggg gatcaaattt cgtattggta tacgtacgcc atcgtttatc
     3301 agaaggagcc tccatccgcc ttccgtagcc cgcgccagcg gcggaagatt cctatccctg
     3361 aatccgcgac tgcgtagcga gttgctgccc tgatggcagt gataatagaa agccgacttc
     3421 gcccgtagca gccgatccag agcggtttct ttctgcgaat catcccaccc aagtttgttc
     3481 gtatcagcta cagaacgacg atgcgtctgt tttgcccaac tcccgccgca tgtcgtcaaa
     3541 ctgttctccc ggttactgga ccagcagctg tgacggagac ttgctgttac attcggcaac
     3601 ccaacggttt atcggcgcct ggatctacta gtccaaactc gccgccgccg ctggtcacct
     3661 ctaacctcaa atgctgctta aggactgagc ggggattgat aatgcattaa cgggatcggc
     3721 tttagtggag cgagcaccac caagccgctt aaagcgtcgg ctgtttcctc agcctgaggg
     3781 agggccgcca ttccctgggg tatttccttt ggttgctcca atgcaccgtg tcgttctgaa
     3841 tgcagcgtca cgttgaattg cgtctccagg gggaaacatc tgctgattca aaaccttgag
     3901 aatgtgcaat agtgctcagc ttctctgtga gatttaccgc atcgttgatt gggtaccgga
     3961 aaatggctcg gcctggaact ccctatagac gacctgtcaa tcgttacaat tcgcgtgaag
     4021 agcttcatgc tcttgggctg ttgtcgcggc aataccttgc tcgttccgct gcgcaaaagg
     4081 atgaggcggc cgcgaacatg gagtctgcgg aatgaaacga gcattcattg accacggttt
     4141 gtcgctgctg gcgggaggga tcctgatgac ggggcccgtg gttgcggtgg cgcaggctct
     4201 tcaggctact ccgcctcgta cggatgcgac gggcaagccg ccttacaaca taatcttagt
     4261 gattgttgac cagaggtcct accgcctgtt cgccggaagc ggctattctc tgccagccat
     4321 caacaccctc gctcgtcacg gagtcacgtt ccgcaaccat tacactgctt cggccatgtg
     4381 ctcaccgtcg cgagcggcgt tgcttactgg acagccgccg caagtcaacg gagtcttgga
     4441 tcagatggag tactccttcg tgtcgagcct cagcacgaac tggcccaata tcggatctgt
     4501 tttgaaggaa ctggcctaca agactgccta cttcggcaag ttcgagatgg acaaagatct
     4561 tctcaaaccc aatgggtcag tcaattacag cgccgccctc ctgccttacg ggtttgacca
     4621 gttcaatgcg gtgggagata tcggcagtgc cccggagagt ggcttcgaca acgatgcctt
     4681 catcgccggt gagagtgtca gttggctgcg ggcgaatgct ccagaagccc gacggagcgg
     4741 tcagcctttt ctactggtgg ccagcttcgt caatcctcac gacgtcatgt acgccaacgc
     4801 caatgttccg ggtcaacccc cagtccagaa agcggtaacg ccgcggtcaa tacccccgct
     4861 gccgtcggat gcgacctacg ataggaaatg gtccttcaca ttccctgcca gcctccagga
     4921 gtcgctaacc gctcccggca taccgaatgc gctactcgaa tatcagcagg gatggtccgg
     4981 atggtcgggg attattccca ccgaccgtaa agatatgtgg ggcatcttct acaactatta
     5041 cctgaacacc atccgagaca atgaccggag tctccagcag gttgtcgatg ccatgaatga
     5101 catggacctg tggcgagata ccgtcataat ctacacctcc gatcatggcg aaatggccgg
     5161 agctcacgga ggattgaagg gtaaaggacc gttcgcctac gaggcaaacg tccacgtacc
     5221 gttgattatc gcgcaccctt cgggaaagcc cggctccagt tgttccgcac ttaccagcca
     5281 tctggaccta cttcccacca tattcggcct gactgggttg ccggagtccc gtcgtcctgc
     5341 ggctgtgaag gcactgccgg gccatgattt ctcagcattg ctcgcaaatc cgcagccggc
     5401 caatgcgcag acggtgcgac ccggcgtcct atttaactat ttagggctta gcaccattga
     5461 tggcgactat ctccgtgagc taatgtccgc cggccaactg caccaggcca caccaccggt
     5521 aacccaggtg aatctcgaca agcgtggcta cctctccttc gtatttgatg gacgctataa
     5581 gttcggccgc tactacgcac cgaacgcttt caatacgccg aagacgctgg aggaaatctt
     5641 taaatataat gacattcaac tattcgattt ggagaatgac cccgacgaaa tgcacaatct
     5701 ggccctcacg ccgaagagga atcaggcttt gatactgcgg atgaatggtc tgctaaacaa
     5761 tcttatggcg aaggaagtcg gtatttacga tgggcgtact atcccccaag tcgtaggctc
     5821 gaagtgatta cttcgtgacc tcgaggaaaa gcgtcgggct ttatcaacgg accggagggg
     5881 gcagttggaa ctggtgtgaa gatggatgcc gcgggagata tctacggggc gacgtacttg
     5941 ggtggattgg tctttaagct gaccccaaag gactccagct ggactgtttc acacaacccc
     6001 agcacaccat tgtgatcggt gttgcagatg cggtaattct caagagccga cccgctaaaa
     6061 ctagtctgcc ggattaagtc cccgcgctac tgcactgcac gagagtggcg ggagatcttc
     6121 gcgagttagt acgaactcgc gagtacagtt cggatgggag cctgctgacg agtgtcatgc
     6181 gttcggcggc tggtgtttca ggaggcgtag agatgaagaa acaggtcgtg atttccacgt
     6241 cgcttatggc tgtgctgctg ttgtccagtg cagttgggtt tggacaacaa gacgcgacga
     6301 gcggaggcga caaattggca ccagcgatgg gcaagaacgt caccagcact gcggccagtt
     6361 acctgtcgtt cgattcgatc gattcggatg gacagtattc ccatgtctca tccggcgtcg
     6421 gcttccgtta tatcggcact tatagcgtca agcggctgaa tcaggtgttg actacggagc
     6481 tgtctgagtt caccaccttc aaggcgacat atcctccggc tcgctatcca gtaaagttgt
     6541 accgaatcat ctatcactcg gttatcccgg aacaggacaa ccggccgacg atcgcgtcag
     6601 ggttgattgc catacctgaa actggccttc ctgccatgcc cctagtgtcg taccagcatg
     6661 ggaccgtatt taccaagaca ggagtgccat cgcatcctga tgagtcgatg gaaacacgat
     6721 tgatgattgc cgagttcgct ggccaaggtt atgccgttat aggggcagat tatttcggca
     6781 agggtctatc tccggaagct gacagctacc tggtcaaagc cagcacccag caggcctgcc
     6841 tcgacatgct atttgccgct cgcgccgtgg gctcggctct taaagtgcag ttcggacctt
     6901 tattcctgag cggctggtcg cagggaggct ggtccaccat ggtgttcctg aataagttga
     6961 acagccttgg catttcggtt aaggcggccg ccatcgccag tgcgccaaat gacctgtttg
     7021 ccattctgaa tcgttggatg cacactccgg ctgcaattga cgccgactac ctgcccgcgt
     7081 tgatcgccct gcaggcaaat gcctatgagg aatactacca attgccaggg ctcgccgcat
     7141 cggcgatcaa aacggaatac ctgcagaccg cgcgtgattt gtatctcaat aaacttacct
     7201 gggagcaggc acagcccaag ctgccaaaac gattgaatga ttttctccaa agcggcttca
     7261 tcattgccag ttcgatcggt gacacgcggt tttatcgaat cgtgcaagac agtcaggact
     7321 atcgctggcg cagcgcaact cccttgcgca cctactatgg cgacatcgac gaagttgttc
     7381 cttcctacat cgccaccttg ccggttcaat atcaggaaat catgggaggg gccaccgtca
     7441 ctgccatcga cgctggtaat cttgcaaatc accgtggaac ttttgtttat ggtgtagctg
     7501 accagaagaa atggtttgat cagctaatcg ctaagtaggt tagtggcgtt aatgcgttcg
     7561 atcgagaact caaggacgaa gagatgttcc tccatcgggg ggcttggaca tgtcgtcgcc
     7621 gcagcacccg tgcgtggctg ctcaatactg gccccgcgtg ccagtctccg gtatagctag
     7681 cgcgcatcgc catgttcgca agtaactcgg gcgatgcgac ctgttcggtc agatcgcgag
     7741 ttggtgtgtt cacgcccggc tatctacgcg ggcccgaagc ctgcttgtgg gagatctccc
     7801 gcggacaatt tcactgagat tcctcccgac cgaggggcaa accgcaaggc acggttcgtc
     7861 gctccgtcgc aagcagtgca agagtcctaa gaggacgttc atcacccgag cgtcgccaca
     7921 ttgccatgac ttccgttgca actagcacac ggccgcagtt tggatgttcg cagatccggt
     7981 gcacattctc gttgcaaccg ggatcgacag ccgcgtgcat ttaatgcgca tccttacgca
     8041 gcactgtgcg cacggcgctg cagactgatc acacagttcg aatctcaaac gagccgtgca
     8101 ccgtgccact cgtcttgcac gaacccatgc gcacgcttcg gctacaacca gcgcgtgagt
     8161 gacaacaact ggcaacggat gtccccaccc acgctggtgg atcagtccac aacacaccgc
     8221 cacgccgctc gaacgatagc cgtgtacatt catcgcccaa accgaaccac tgaggttcgc
     8281 acagtccgcg cgtgtcgcgg caccagcttg tgcaacttgc gcgtgcacag ccctcagcga
     8341 tcacccaaaa ccggccaatg agtatcacct gaaaaccggc taacgagagc agcgtctggc
     8401 acatgtccag caaccgctgg ttcaactcct cccaactggc caccttcgga atcggcacta
     8461 gaaagttccg ccgtatgtac cccaccaagc cttccacttt gcctttgtcg ttgcccttgg
     8521 ccggacgtcc gaacttgtcg gcaaacagat agtggc
//
LOCUS       DASRKK010000201         5279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_991790, whole genome shotgun sequence.
ACCESSION   DASRKK010000201 DASRKK010000000
VERSION     DASRKK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991790"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /gene="asnB"
                     /locus_tag="VFC15_04435"
     CDS             complement(<1..1412)
                     /gene="asnB"
                     /locus_tag="VFC15_04435"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZM09439.1"
                     /translation="MGILSDGKPEQTRAAVRDMIAASRHRGPDASGSMEIPLATGSLI
                     LGHTRLSIIDLSDGSRQPMQDAESGSWLAFNGEIYNFRQLRAELETLGTKFATSGDSE
                     VLLKALVQWGEAALNKVEGMFAFAFWDGRGRSLLLARDRMGIKPLYYYSGRRGFSFAS
                     EVKVLERAHLGPLTLDRDAVDSFLAYGAVIGPNTIFKEIRELEPGHVLRINARGDVAD
                     SEYWSLSRSLAESTTAEPQDFEQAVSQIRERLGYAVDSHLVSDVPVGVFLSGGVDSSL
                     LALLASHYAKSPITLLTVAFAEQEFSELPYARQIARGLPHHHEVVMLTAEQLRESLSA
                     ALSAMDQPTVDGINTYVISHVGASLGLKVLLSGIGGDELFGGYTTFTKVPRLLRYGAG
                     LRPAARVLAKLRTKNPIPWKKVADAGPLHSLTEAYLLQRSIRWQQNGSRTLGHRLQEN
                     IEPLADDFQKVAALELQFYMR"
     gene            complement(1431..2234)
                     /locus_tag="VFC15_04440"
     CDS             complement(1431..2234)
                     /locus_tag="VFC15_04440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09440.1"
                     /translation="MRYDWKGGIPYDEFSKEFYAEIDRRFFTAVELYAPCKKIPFDWL
                     MDFDSLRTKDVLEVGVGNGSHAQLLAEHAKSFTGIDLTEYAVRSTSERMRAFGLAGTV
                     AQMDAEQMQFPDNSFDLVWTWGVIHHSANTRKIVEEMHRVLRPGGEAFVMVYHRTLWE
                     YYVQGVLLATLSGQIFKPAALHESIQRRTDGAMARYYKASEWRQLVSDLFLVKEIRIY
                     GKKTELAPLPAGKLKDLLVGLMPNSFSRFLTNKCRWGSFLVSRLVKEAG"
     gene            complement(2360..3556)
                     /locus_tag="VFC15_04445"
     CDS             complement(2360..3556)
                     /locus_tag="VFC15_04445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM09441.1"
                     /translation="MKVLILATDVFSRGGIARYTSTLASSLASMLGPENVDVLCFFDW
                     GYSGELPSEFRVLGTVSGRGRAGVLSRLNFLFKAARFGTRGYDLVIANHVALAPVAAL
                     MKLAFRTPYWVACHSVEVWWGTSRLRHAALKNADLILPVSRYTADAVQKMAGIRSSRV
                     KVVYNAIPNSFAKLLLSEEPVGPSIGKLKRGGSLLLSVCSLVRGNEFKGVDTIIRALP
                     KILKTQPDLRYVVVGEGEIRTGLENIAVEMGVAGNVTFLGEIPDSELAELYRLCDAFV
                     LPSRGQERLGTVGGEGFGRVYVEAALAGKPVVGSLSGGAAEAVLHGKTGFLVNPDSSD
                     EVAEAVLAILEDPRLAAAMGSVGREWALDTFSEEALSRSLKELLRPYGIENERLPKLV
                     HASGQR"
     gene            complement(3659..5056)
                     /locus_tag="VFC15_04450"
     CDS             complement(3659..5056)
                     /locus_tag="VFC15_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09442.1"
                     /translation="MPGFAGLRTDALTWVIMAGMTACWMISIRLVPNWKRDGWTSLLK
                     SVYGIVWLDFGLDIVLRFSMLAYNAVEWGNGGLRLVAQPVDTVNTTLEYCALFWLLIA
                     LAYAVAVRRRGAGPLGLARSFTPEFAYAAAIPVALLASVVFYLIEGQSRLPLALITPL
                     ALLANLYMVPAAIVWWDHFRRPGPKWHIGSIHLLVLLPALVRGVCSPYRENLAPILLI
                     PLIAAMFAGKRPSLMKVVPAGLVCLLLLSTVVSSYRRIKWQNVRPEEVAREYGEGGLG
                     GWLSGASDEPVRRFHAFDSLLLTVALVPSLEPYSGRNVLLSPLVRGVVPRFIYSGKSG
                     ADAGVNFGPRIWAFDDPVTRENSGASIAPSMPGDLFDAGGVLYIALGALMWGTLLGLV
                     DGWKAHLPPFCGAAITLLVATQCAMSVERDFDNSIATFIQTLLVFVLVGGLLALARRG
                     STDFSHGLNPSLERS"
BASE COUNT         1146 a         1666 c         1459 g         1008 t
ORIGIN      
        1 cgcatgtaga attgcagctc cagggcggca accttctgaa aatcgtcggc cagaggttcg
       61 atattttcct gaagacggtg ccccagtgtc ctcgaaccgt tctgctgcca gcgaatgctg
      121 cgctggagca ggtaagcctc cgttaagctg tgcagcggac ctgcatcggc cactttcttc
      181 cacgggatcg ggttcttcgt tctcaacttt gcaagtacac gcgctgctgg acgcagcccc
      241 gctccgtatc gcagaagcct gggcaccttg gtgaacgtcg tgtagccgcc gaacaattcg
      301 tcgccaccaa tcccgctcag cagaactttc aaccccagcg aagcacccac gtgtgagatc
      361 acgtaggtat tgatcccgtc cacggtcggc tggtccatgg cgctcagcgc cgccgaaagc
      421 gactcgcgca gttgttccgc ggtcagcatt accacctcgt ggtgatgagg caggccgcga
      481 gcaatttgcc gggcgtaagg cagctcggag aactcctgct cggcgaacgc aaccgtcaat
      541 agcgtgatgg gactcttcgc gtagtgcgac gcaagcagcg ccaacaggct ggagtccaca
      601 ccgccggaga gaaacactcc gaccggaacg tcgctaacca ggtgggaatc gaccgcgtat
      661 cccagacgtt cccgtatttg tgacaccgcc tgctcgaaat cctggggttc cgctgtcgta
      721 ctctcggcaa ggctgcggct taacgaccaa tattcggagt cggcaacgtc gccgcgtgca
      781 ttgatccgta gcacgtggcc gggctcaagt tcccggattt ccttgaagat cgtattcggc
      841 ccaatcaccg cgccgtacgc caggaacgag tcaactgcgt cccggtcgag agtcagcggg
      901 ccaagatggg cccgctcgag gaccttcact tccgaggcaa aggaaaatcc ccgccgcccc
      961 gagtaatagt aaagaggctt tatgcccatc cgatcgcggg cgagcagaag acttcggccc
     1021 cggccatccc agaaggcaaa cgcgaacatg ccttccactt tgttgagcgc cgcttcgccc
     1081 cattgcacca gcgccttcag caagacttcg gagtctccgg aagtcgcgaa cttcgttccc
     1141 agagtttcca gttcggcgcg caactggcgg aagttgtaga tctcgccgtt gaacgccagc
     1201 cacgagccgg attcggcatc ctgcatcggc tgccgtgagc catcgctaag gtcgatgatg
     1261 gaaagccggg tgtgccccag gatgaggctg cctgtcgcca gcggaatctc catgctcccg
     1321 ctggcatcgg gaccgcggtg gcgggaggcg gcaatcatgt cgcgcaccgc cgcccgagtc
     1381 tgctcaggct tgccgtcgct caaaattccc atgattccgc acataggttg ctaccctgcc
     1441 tctttaacga gccttgacac caggaacgag ccccatcggc atttgttagt gagaaaacgg
     1501 ctgaaggagt tcggcattaa tccgacgagc aggtccttga gctttcctgc tggcagtggg
     1561 gcgagttcag ttttctttcc gtaaatacga atttccttga ctaggaatag gtcggagacc
     1621 aactgcctcc actccgaggc cttgtaatat cgcgccatcg cgccatcggt tcttctctgg
     1681 atactctcgt gaagtgctgc tggcttgaaa atctggcctg atagggtcgc tagcaagact
     1741 ccctgcacgt agtattccca gagcgtgcgg tgatagacca tcacgaaagc ttctccgcct
     1801 ggcctcaaaa cccggtgcat ctcctcgacg atcttccgag tgtttgccga gtggtgaata
     1861 acgccccagg tccaaacgag atcgaaactg ttgtctggga actgcatctg ctcggcgtcc
     1921 atttgtgcca ccgtcccggc aaggccaaag gctcgcattc tttccgatgt gctcctgacc
     1981 gcatattcag tgagatcgat acccgtgaaa gatttggcat gctccgcaag tagctgggca
     2041 tggctgccgt tgccgacgcc gacctccagt acgtccttcg ttcggagaga atcgaagtcc
     2101 ataagccagt cgaaggggat cttcttgcaa ggcgcataaa gctccacagc ggtgaaaaac
     2161 cgcctatcaa tttcggcata aaactcttta ctgaactcgt cgtagggaat accgcctttc
     2221 cagtcgtagc gcatgggatg gctttcccac caatcacgat tggcgtcctg ccagccccgc
     2281 tcttcgtccg tactcgccgg aagcgcagta ggctggctga aacccctggg cagttcttcg
     2341 agttccttca tgagcttcgt catcgctgtc cgctcgcatg cactaacttg gggagccttt
     2401 cgttctcgat gccgtaaggt cgcaacaact ccttcagcga tctggaaagg gcctcctcgg
     2461 aaaacgtgtc gagcgcccat tccctcccta cagagcccat agcggccgca agccgagggt
     2521 cttccagaat ggcgagcacc gcctctgcta cctcgtcact cgaatctgga tttaccagga
     2581 aacctgtttt tccgtgaagc acagcttcgg ctgcgccgcc ggatagactg ccgactaccg
     2641 gctttccagc cagcgccgcc tccacgtaga cgcggccaaa tccctcgccg ccgactgtcc
     2701 ccaggcgctc ctgccctcga ctcggcagca cgaacgcatc acagaggcga tagagttcgg
     2761 ccaattcgga gtcaggaatt tcccccagga aggtgacatt tccagccaca cccatttcca
     2821 ccgcgatgtt ctccagcccc gtcctgatct cgccctcacc cacgactacg tatcgcaggt
     2881 cgggctgtgt ctttaagatc ttcggtaagg cccggattat cgtgtcgacg cccttgaact
     2941 cgttccctcg aaccaagctg cacactgaca ggagaaggga accgccccgt ttgagctttc
     3001 cgattgaagg gcccactggc tcttccgaca gcaacaactt cgcgaaggaa ttaggaattg
     3061 cgttgtacac caccttcacg cgcgagctcc ggattccggc catcttctgc actgcatccg
     3121 cggtatagcg actgactgga agaataagat cggcgttctt caaagcagca tgtctcaacc
     3181 gcgaggtgcc ccaccaaact tccacactgt ggcatgccac ccaatatgga gtcctaaaag
     3241 ctaacttcat cagcgctgcc accggcgcga gggccacatg gttcgctatg acaaggtcat
     3301 atcctcttgt tccgaaccgc gcggctttga ataggaaatt tagcctggac aggactccgg
     3361 cgcgcccgcg accgctaacc gttccgagca cgcggaattc actcgggagt tcgcccgaat
     3421 atccccaatc gaaaaaacat aagacatcca cattttccgg accgagcatg ctcgcaagcg
     3481 aagaagccag cgtcgaggtg tacctcgcga tcccaccacg cgaaaaaaca tcggtcgcca
     3541 gaatgaggac cttcatgcga ggatgtcctc cccgcggctc aacgggcgca gagcggcctc
     3601 gcagttcttc agtttcgggg agatggatgg gctggccaga atggttgtca tgggttctct
     3661 aggaacgctc cagactggga ttcaacccat gcgaaaagtc ggtgctgcct cggcgggcca
     3721 aggcgagcag accgccaacc agaacgaaaa ccagcagcgt ctgaataaac gtagcgatgg
     3781 aattatcgaa gtcgcgctcc accgacatgg cgcactgggt tgccaccaac agcgtaatgg
     3841 ccgcgccgca gaacggcgga agatgggctt tccatccatc caccaatccc agcaacgttc
     3901 cccacatcag cgctcccaac gcaatgtaga gcactccgcc cgcatcgaag aggtcgccgg
     3961 gcatggacgg agcgatggag gcgccggagt tctcgcgggt tacgggatcg tcgaacgccc
     4021 agatcctcgg cccgaaattc acgcctgcat cggcgccact cttgccgctg tagatgaaac
     4081 gcggcaccac cccgcgaacc aacggggaca gcagcacatt ccgccccgag tacggctcca
     4141 gggaagggac cagagccacc gtcagcaaca gggagtcgaa ggcgtggaag cggcgcaccg
     4201 gttcgtccga agctccgctg agccaaccgc cgaggccgcc ttcgccgtac tcgcgcgcca
     4261 cttcctcggg acgaacgttc tgccacttga tgcgccgata ggaactgacc acggtgctga
     4321 gcaacaacaa acacaccagg ccggctggaa ctactttcat cagcgacggc cgcttgcccg
     4381 cgaacatggc cgcaatcaag gggatcagga gaatgggcgc caggttctcg cgatagggac
     4441 tgcacacccc gcgcaccaat gccggcagca gcaccagcag atgaatgctt ccgatgtgcc
     4501 acttgggacc gggccggcgg aaatggtccc accagacgat cgcagccggc accatgtaga
     4561 ggttcgccag cagagccagc ggcgtgatca acgccagtgg caggcggctt tgcccttcga
     4621 tcaaatagaa gacgacagaa gccagcagcg ccaccgggat ggctgcggcg taagcaaact
     4681 cgggcgtaaa cgaccgcgcc aggcccagcg gccccgctcc acggcggcgc accgcgactg
     4741 cgtaggccag cgcgatcagc agccagaaca atgcgcagta ctccagcgtt gtgttgaccg
     4801 tatcgaccgg ctgcgccacc aaacgaagcc ccccgtttcc ccactccacc gcgttgtagg
     4861 ccagcatgga gaagcgcagc acgatgtcca ggccgaagtc gagccagaca atgccgtaca
     4921 ccgacttcaa cagcgaagtc catccatcgc gcttccagtt cgggacgagg cgaatcgaaa
     4981 tcatccagca ggcggtcatg cctgccatga tgacccaggt gagagcgtcc gttcgtaaac
     5041 cggcaaaacc gggcatagtg ctttcttccg tctggaacgt tcttggttat gcagcgcgca
     5101 gtcgcgggcc tacactgcga ccggctacga tgcgccaacc cacgctggcg atggacatat
     5161 caaattgggg aattcaactc ggggccagag tgaaatccgg aaggaaaatt gtccagcgtc
     5221 gggggtaagt ctatcggcat ggtctttttt ttcatatggc atgaaagttg taggtacat
//
LOCUS       DASRKK010000202         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1187370, whole genome shotgun sequence.
ACCESSION   DASRKK010000202 DASRKK010000000
VERSION     DASRKK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187370"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFC15_04455"
     CDS             <1..820
                     /locus_tag="VFC15_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09443.1"
                     /translation="VASGLMDSEKALLESRGLLNPQGKITPSIISKDALDTPEKLLSR
                     ELWNAIYGVPKGDITIEHIQHAFYMAANYGFQILNGNFAAAIDDYELKQRFMNDLATY
                     RIDVSWLWTLVSHQAVITKDGYFQRSALTEDGVVLAPSTEQVMAGTRFTKELFQKVLD
                     YHNEWTAEFFAEQDRRGDPGRFDRAKAPLIMVLLKRQLLSPKYIQHSARVLFLLAQAN
                     DEEREQILAAIFDVSHQEIVKRMQAGTMKPAALVVHDHVYDIFPVPKERLVAMP"
     gene            1046..1882
                     /locus_tag="VFC15_04460"
     CDS             1046..1882
                     /locus_tag="VFC15_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09444.1"
                     /translation="MTTPTETCPICGGTGWQTIERGKEREAVRCECRVRDRGERLLTA
                     AHIPSRYAGCDLTNFNTEGERETLLDAKRTAEQFAENYPLEKRGLLFVGSPGVGKTHL
                     AVSILKRLMGDKGIQCRFVDYRELLKQIQYTYNPTVQMTELELLQPVFETEVVLLDEL
                     GAIRTSQWVWDTVAHILNNRYAENRTTLITTNFEDRAERALEEKERGEHFTDPERAVR
                     GETLGDRIGGAMWSRLHEMCQKVDMVGGDYRRRFYSTRLVRKRMSVKSHKDEPPPAVE
                     EN"
     gene            2144..3373
                     /locus_tag="VFC15_04465"
     CDS             2144..3373
                     /locus_tag="VFC15_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZM09445.1"
                     /translation="MKYNSLVLSHRIKFAPYRDSDLFAAVPASAAVFLLRGEDEAGEP
                     YVSKTSNLRRRLQRLLGPLDGQTRKLNLRERVRWVEWAATGSDFEASFLLYQTLRREF
                     PKTYDKRLRLRFAPLVKLILGNPYPRATVTTRISGGKGNAQYFGPFATRVAAEKFAND
                     ALDLFKMRRCIDDLHPDPQFPGCIYSEMKMCLAPCFKGCSDEEYAAEVGRAQQFLETG
                     GQSLVREIERQRNEASTNLDFEAAAALHTRLEKVKAATAQLPEIVRRLDELNGVMVQP
                     SAERESVALFKIVAGRICDPVALNVGQPAGAAHLTSKPQSMESRITEALAVVEAPKLR
                     SALEWMEYLALLKRWYYRTIKVGELFLAENGELPMRRVVRGVSRVFRGEKPAGDLSET
                     AGDYWKFRAREAGLNQT"
     gene            complement(3511..>3815)
                     /locus_tag="VFC15_04470"
     CDS             complement(3511..>3815)
                     /locus_tag="VFC15_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZM09446.1"
                     /translation="LESVYREAMIVALRSKNVPVEREKTVNVEFRGETVGVFRTDLVV
                     ENSVVVELKCARTIDSNHEAQLLNYLKATKFEVGLLLNFGVKPQFRRMILETARNR"
BASE COUNT          807 a         1125 c         1115 g          768 t
ORIGIN      
        1 cgttgccagt ggcctgatgg actcggaaaa ggcgctgctc gaatcccgcg gcttgctgaa
       61 cccgcagggc aagatcacgc cgagcatcat tagcaaggac gcgttggaca cgccggagaa
      121 gctcttgtcg cgtgagctgt ggaacgccat ctacggcgtg ccgaaaggcg acatcacaat
      181 tgagcacatc cagcacgcgt tctacatggc cgctaactac ggtttccaga tcctgaacgg
      241 caacttcgcc gctgccatcg acgactacga actcaagcag cgcttcatga acgatttggc
      301 cacgtaccgc atcgacgttt cgtggctgtg gacgctggtt tctcatcagg cggtcatcac
      361 caaggatgga tattttcagc ggtcggcgct cacggaagac ggcgtggtac tggcgcccag
      421 caccgagcag gtcatggcgg gaacccgctt caccaaggag ctgttccaga aggtgttgga
      481 ttatcacaac gaatggaccg cggagttctt cgccgaacag gaccggcgcg gcgatccggg
      541 acgctttgac cgggcgaaag cacccctcat catggtcctg ctgaagcggc aactgctttc
      601 gccgaaatac atacaacaca gcgcgcgcgt gctgttcttg ttggcgcagg ccaacgatga
      661 ggagagggaa caaatcctgg cagccatctt cgatgtttcg caccaggaaa tcgtgaagcg
      721 gatgcaggca ggcaccatga aacccgccgc tttggtcgtt catgatcatg tttacgacat
      781 ttttcccgtg ccgaaagagc gtctcgtcgc catgccatga catctcgagt cagcggaaca
      841 aaagacaacc gcagattgcg cggaggacgc ggattgtctc agggtttctt tcttgcagac
      901 ttatccgcga aatccgcgtg aatccgcggt taagactttg ctctccccga tttacacagg
      961 cgtgcacagc ttttacggtc actgtgcgca atctccttcg ctatcgccat tgcgcgacgc
     1021 cccgcccggc agtagattca aatttgtgac caccccgacc gagacatgtc ccatctgcgg
     1081 cggtaccgga tggcaaacca ttgagcgcgg caaagagcgc gaagcggtgc gctgtgaatg
     1141 ccgcgtgcgg gaccgcggcg agcgtctgct gaccgccgcg cacatcccgt cccgctacgc
     1201 tggttgcgat ctgacaaatt tcaataccga aggcgagcgc gaaacgttgc tggatgccaa
     1261 gcgaacggcc gagcaattcg ccgagaacta cccgctggaa aaaagaggcc tcctttttgt
     1321 cggctctcct ggggttggca agacccatct agccgtgtca attctgaagc gactgatggg
     1381 cgacaaaggt atccaatgcc gctttgtaga ttatcgcgag cttctcaagc agattcaata
     1441 tacgtacaac ccgacggtac agatgacgga actggaactg ctacagccgg tctttgagac
     1501 cgaggtggtg ttgctcgatg agctcggggc aatcagaact tcacaatggg tttgggacac
     1561 cgtcgctcat atcttgaaca atcgctacgc cgagaataga acaacgctca tcacgacgaa
     1621 tttcgaagac agggctgaga gagctttgga ggagaaagaa cggggagagc atttcacaga
     1681 ccccgaacgc gcggtgcgcg gcgagactct gggagatcga attggcgggg cgatgtggtc
     1741 gcgattgcat gaaatgtgcc agaaggtaga tatggtggga ggcgactatc ggaggcgctt
     1801 ctactcaacc cgcctcgtga gaaaacgcat gagcgtaaag tcacacaaag acgaaccccc
     1861 gccggcggtc gaagagaact agcggttcct ctgtgcctgc gttgctaacc tttgggtctg
     1921 cgcagcggac tacgtctttc ttagccaata aaaacgggtg tcatgctgag cggagcctgc
     1981 cccgcctttt gggcaggcgg agtcgaagca cccctattcg accccggaca tacgggtggg
     2041 aataggggcc cttcgactcg cggctaaacc cgccgctcgc tcagggtgac aggcggtttg
     2101 gtttctagct ttggttttct ccgtgccctc cgtgtgctcc gtggtgaaat acaattccct
     2161 agtgctctcc caccgcatta aatttgctcc ttaccgtgac tctgacctgt tcgccgctgt
     2221 gccggcgagt gcggcggtgt ttctgttgcg tggagaggat gaagcgggcg agccgtacgt
     2281 cagcaagact tcgaacctgc gccgccgctt gcaacgcctg cttggccccc tcgacggaca
     2341 aacccgcaag ctgaacctgc gcgagcgcgt tcggtgggtc gagtgggccg ccaccggctc
     2401 cgacttcgaa gcgtcgttcc tgctctacca gacgctgcgc cgcgagttcc ccaagaccta
     2461 cgacaaacgc ttgcgcctgc gtttcgcgcc attggtcaag ctcatcctcg gcaatcctta
     2521 cccgcgtgcg acggtcacca cgcgcatcag cggagggaag ggcaacgcgc aatattttgg
     2581 gccgttcgcc acccgcgtcg ccgccgagaa gtttgccaac gatgcgctcg acctgttcaa
     2641 gatgcgccgc tgcatcgacg atctccatcc cgacccgcaa ttccccggct gcatttattc
     2701 cgagatgaag atgtgcctgg cgccttgctt caagggatgc agcgacgagg aatacgcggc
     2761 cgaagtgggg cgcgcgcaac agtttttgga aaccggcggg cagtcgctgg tgcgcgagat
     2821 cgagcggcag cgcaacgagg cttcgaccaa tctcgatttc gaggccgccg ccgccctgca
     2881 cacccgcctg gagaaggtga aggccgcgac cgcgcaattg ccggaaatcg tgcgccgtct
     2941 cgacgaactg aatggcgtga tggtgcagcc gtcggcggaa cgcgaatcgg tggcgctgtt
     3001 caaaattgtt gcgggccgca tctgcgatcc cgtagccctc aacgtcggcc aaccagccgg
     3061 agctgcgcat ctcacttcga aaccgcagtc gatggagtcg cgcatcaccg aggcgctcgc
     3121 cgtggtcgaa gcccccaagc tgcgctctgc gctggagtgg atggaatacc tcgcactgct
     3181 gaagcgctgg tactaccgca ccatcaaggt cggagaattg tttcttgccg aaaacggcga
     3241 gctgccaatg cggcgcgtgg tgcgtggcgt gtcgcgggtc tttcgcggcg agaagcccgc
     3301 cggtgacttg agcgagaccg cgggagacta ctggaaattc cgggcccgtg aggccgggct
     3361 taatcaaact tgaacaaacc aaccgcggat cacgcggagt tcgcggatga ttctttgtgt
     3421 tcttgttctc tctcggcaag gaaggatccg cgcgatctac gccttgtgcg ggtagtcttt
     3481 ttgagctcca gattcgcaga acgctccaag ctagcgatta cgggcagttt ccagaatcat
     3541 tcggcggaac tgcggcttca cgccgaagtt caacagcagt ccaacctcaa atttcgtcgc
     3601 cttcaggtaa ttcagcaact gtgcttcatg attgctgtca attgtccggg cgcactttag
     3661 ttcgacaaca acagagttct ccacgaccaa gtcggtgcgg aacactccaa cagtttcgcc
     3721 acggaactcg acattcacgg ttttctcccg ctccaccggc acattcttcg agcgcagcgc
     3781 gacaatcatg gcttcgcggt acaccgattc cagaa
//
LOCUS       DASRKK010000203         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1187545, whole genome shotgun sequence.
ACCESSION   DASRKK010000203 DASRKK010000000
VERSION     DASRKK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187545"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="tsaE"
                     /locus_tag="VFC15_04475"
     CDS             <1..562
                     /gene="tsaE"
                     /locus_tag="VFC15_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZM09447.1"
                     /translation="VNNFHRETANWPLITRYRAVALDPSLHGEQKPRPAKIGLRSAMN
                     THEHITHSAEETMALGRKLAPTLKAARMVILRGDLGTGKTTLVKGIAEGLHAASQDDV
                     TSPTFTLIHEYRGPEVNLFHVDLYRIDTPRELDTLGLDELFAEPGNLVLLEWGEKFPR
                     FEKERDVEICLERQGDQERKIVVKRK"
     gene            570..1727
                     /locus_tag="VFC15_04480"
     CDS             570..1727
                     /locus_tag="VFC15_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09448.1"
                     /translation="MPRHPWPSALNKAALCLVLSIWVLAPTCVAESIRAGCATPLWAA
                     EVLENLSGARNLPTPELIDKNRAGITFLDEEKLLVYEVDVNTGRLSSRESPDVSSPYR
                     LRARVFDIHSGKVIVSKEWGTRVHGSAIQVTSGGVLVQTGELLRLYSRDFVHLQELTL
                     PHRDADDTDIISVSASGKTVLINRYNQKFSRFDVLDGDTLNLRQRWSEAPPLRRLYSI
                     SDTGIAAADFNQEHVLFTKFGTGAWQLVVGKPKLTCVGLPALVTDSSLVIGCKGLSLI
                     STDGNFIYQDGFGKGEALRPEIAISQNDKTVAASLDVTRGTDFWDTGKGIRLVAMYVI
                     AYDLSLKKRSLKVEVAPVPKNDYDFALSPEGSRLAILTDRRVTVCSVAAAQ"
     gene            complement(1861..2091)
                     /locus_tag="VFC15_04485"
     CDS             complement(1861..2091)
                     /locus_tag="VFC15_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZM09449.1"
                     /translation="MKKLTKEQKREIAAVAAKKDEDIHLSEMPEVIDWRGAEMGKFYR
                     PPKRPVTMHLDADVVEWLRATDPVIRPRRTCF"
BASE COUNT          498 a          643 c          620 g          518 t
ORIGIN      
        1 cgtcaacaat tttcatcgcg aaactgccaa ctggccattg atcactcgtt atcgagccgt
       61 cgccctcgat cccagcctgc acggcgaaca gaaaccccgt cctgctaaaa ttggccttcg
      121 ttccgcgatg aacacccacg agcacatcac gcactcggcc gaagaaacca tggcgctggg
      181 ccggaaactg gcgcccacgc tgaaggctgc gcgtatggtc atcctgcgcg gcgatctcgg
      241 caccggcaag accacgctgg tgaaaggcat cgccgaaggt ctccacgccg cttcgcagga
      301 cgatgtcacc agccctacgt tcaccctcat ccacgagtat cgcggccccg aggtgaacct
      361 gttccacgtc gacctctacc gcatcgacac gccgcgcgag ctcgacacgc tgggcctcga
      421 cgaactcttc gccgagcccg gcaacctggt gctgctggag tggggagaga agttcccccg
      481 cttcgagaag gagcgcgacg tggaaatctg cctcgagcgc caaggcgacc aggagcggaa
      541 gattgtggtg aagaggaagt agggtcaaaa tgcccagaca tccctggcca tcagcattga
      601 acaaggcagc tttgtgtctg gtgctttcga tttgggtttt ggcacccacc tgtgttgctg
      661 agtccattcg agcgggctgt gcgactccgc tttgggccgc ggaagttctt gaaaatctga
      721 gcggtgcgcg aaatctccca acccctgagt tgatcgacaa aaaccgtgcc ggtatcacat
      781 ttctcgatga agaaaagctt ctggtgtacg aagtcgacgt aaatacaggt cgtctttcct
      841 cacgcgagag ccctgatgtt tcaagtccgt atcggctccg agcgcgagtt tttgatattc
      901 attcgggaaa ggtcatcgtt tccaaggaat ggggaacacg agttcacggg tcagcaattc
      961 aagttacgag cggtggagtg ttggttcaaa ccggggagct attaaggctt tactcccgag
     1021 actttgtcca tctgcaggaa ctgactcttc ctcatcgcga cgccgacgac acggacatca
     1081 tttcggtatc cgcaagcgga aagacagtat tgatcaaccg ttacaaccaa aaattcagcc
     1141 gctttgatgt gttggacggc gacactctta acctcaggca acgttggagc gaagctccgc
     1201 cgctgcgacg cttgtattcg atctcagata ctggaatcgc tgccgcggac ttcaatcagg
     1261 agcacgtact gtttaccaag ttcggtaccg gtgcatggca acttgttgtt ggcaaaccga
     1321 agctaacttg cgttggttta cctgcattgg tcactgatag ctcgttagtg atcggatgca
     1381 aaggattgtc tctcatttca acggacggaa actttatcta tcaggatggt tttggtaagg
     1441 gagaagcatt acgacccgag atcgctattt cgcagaacga caaaacggtt gccgcaagtc
     1501 ttgatgtcac aaggggtact gatttctggg atacaggcaa aggcattcgg cttgtggcga
     1561 tgtatgtgat cgcatatgac ctgtcgctca aaaagcgcag tcttaaagtg gaagtagcac
     1621 ctgttccgaa gaatgactat gactttgcct tgtcacccga aggatcgaga ttggcaattt
     1681 tgaccgaccg cagggtcacc gtctgttcgg tcgcggccgc ccagtagtaa atgctgctcg
     1741 aagatgatcc cacctcgatt ctctgggtag caacgcagcc ctgtgcaata atacgaagcc
     1801 actacgcccg gggccgtttt ccggcgggct tcgacggccg ggtgctactc atcgcgtgtc
     1861 tcagaagcag gttcgccttg gtctgataac cgggtccgta gctctcagcc attcgaccac
     1921 gtcggcgtcc agatgcatcg tgactggcct cttcggaggg cgatagaact tgcccatctc
     1981 ggcacccctc cagtcgatga cctccggcat ctcggaaaga tgaatgtcct cgtctttctt
     2041 tgctgcgaca gcggcgattt cgcgtttctg ttcttttgta agcttcttca tgactctctt
     2101 tcggcgtcgt gccggcaacc cgcaaatgcg agaggatcac cctcgggcaa acgtttcaac
     2161 cagcgcagag gaaactgcct gcgctgcgag gggtgagatc tttcccagct tcttcagcag
     2221 tcgctgccgg tccacagcgc ggagttgatc cacagccacc tgtccgcgtc tgccttgaa
//
LOCUS       DASRKK010000204         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_699019, whole genome shotgun sequence.
ACCESSION   DASRKK010000204 DASRKK010000000
VERSION     DASRKK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699019"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..436
                     /locus_tag="VFC15_04490"
     CDS             167..436
                     /locus_tag="VFC15_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09450.1"
                     /translation="MMKYQRLKNLVILVTAVAYFAATFLGQQMKLRILCGKLMIISQR
                     FFGIPPFRFYALADGIRRILSQGTFSPPEMPPPSRQMELTLGWEG"
     gene            456..728
                     /locus_tag="VFC15_04495"
     CDS             456..728
                     /locus_tag="VFC15_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09451.1"
                     /translation="MNMTLAQLQNSVQFRKITPAPTTAGVTPIGQVMWLPDSLIANTN
                     AAFETNGKTWSDLSPFSANRRSRSAIFEDSTRQLMELASENLRKCV"
     gene            815..>2154
                     /locus_tag="VFC15_04500"
     CDS             815..>2154
                     /locus_tag="VFC15_04500"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM09452.1"
                     /translation="MFLRSSVVKKGGKTYRYWKLVENVRTDTGPRQQVVAHLGDLSNF
                     SAADWQALAGRMGEPEMAAALERRVRQGGRQGRPPKWTVEDRSASASNASDVVSIRLS
                     ETSWREPVAFGDVYTALVLWKRLGLGELLSERMNGSAAKVPWPAVAALIAVNRLVEPM
                     PEWPMVRWWQRTALPQLLGIPPTAINDDRLYRCLDLVLPHKQAMEERIAGAGQSLFGQ
                     SYRYLLYDLTSTYFEGQMESNPKAVRGYSRDHRPDCKQVTIGAVVDREGYPVGYEVLA
                     GNVRDHKTVAGMLQRLGARFGLTDRTLCMDRGMVTEDSLKLIRESTVRYVLADRRGAS
                     ERFPEQVQHGPWQTKRADPDTGEAMVEVQEVGAEEGDRLILVRSRGCAQKEKGIHDRM
                     LSKLKAHWERLENTVSKGRLVNPEKIDRRIGSILARHPGMSSWVCVRREELPAAT"
BASE COUNT          458 a          614 c          665 g          417 t
ORIGIN      
        1 atgcggcgga ccggattgag ggacttgaga aacaaatcat actctaaacg aaacgcggac
       61 ttttgtccag ccccttgttg gaaggccctg ttttattggg gtccaacgcg gtaaacgaat
      121 acctttccgt ttcatcaaac agagctacaa cctggaagat atccgcgtga tgaagtacca
      181 gcgattgaag aacttagtca tactcgtgac ggccgtggct tacttcgccg ccacgtttct
      241 cggccagcag atgaagctgc gcatcctctg cggaaagttg atgatcatct cgcagcgctt
      301 cttcggcatt ccgccgttcc ggttctatgc actggccgat ggaatccgaa ggattctctc
      361 ccaaggcacc ttcagtccgc ctgaaatgcc gccccccagc cgacagatgg aactgaccct
      421 tggctgggag ggctgagaaa tgtggggaaa ctcctgtgaa catgaccctt gcgcagttac
      481 agaattcggt gcaattccgc aagattactc ccgcgcccac taccgccgga gtaactccca
      541 tcgggcaagt catgtggctg ccggatagcc tcatcgccaa caccaacgcg gccttcgaga
      601 ccaacggcaa gacgtggtcc gaccttagcc cgttttccgc aaacagacgt agtagatcgg
      661 ccatttttga agattcgacg cggcaactaa tggagttagc ttctgaaaat ctcagaaaat
      721 gcgtgtagtg acaaatcgta tttgacctct tgatctacgc cgatacctct gtcattattc
      781 tcctgagctg ccattcgcag ctcgggtgac aaccatgttt ctccgcagtt cggtcgtcaa
      841 gaagggtggc aaaacctacc gctactggaa acttgtcgag aacgttcgca ccgacaccgg
      901 tccccggcag caggtcgtcg cccacttagg agacctttcc aatttctccg cagcggattg
      961 gcaggctctc gccgggcgca tgggcgagcc ggaaatggcc gccgctctcg aacgccgggt
     1021 ccgtcaggga gggcgccagg gccgtccccc caaatggacc gtcgaggatc gctcggcgtc
     1081 tgcctccaat gcctcggatg tggtttccat ccgcctgtcg gaaaccagtt ggcgtgagcc
     1141 cgtggcgttc ggcgatgtct acaccgcgct ggttttgtgg aagcggctcg ggctggggga
     1201 actgctcagc gagcgcatga acggctccgc cgcgaaggtg ccctggccgg cggtggccgc
     1261 gttgatcgcg gtgaaccggt tggtcgagcc gatgccggaa tggccgatgg tgcgctggtg
     1321 gcaacggacc gccctgcccc agctcttggg aatcccgccg acggccatca acgacgaccg
     1381 tctttaccgc tgtctggatc tggtgctgcc ccacaagcaa gccatggagg agcgcattgc
     1441 cggcgcggga cagagtttat tcggacaatc gtatcggtat ctactatacg acctgacgag
     1501 cacttacttc gagggacaaa tggagagtaa cccgaaggcg gtgcggggtt actcccgaga
     1561 tcaccggccg gactgcaaac aagtcaccat cggggcggtg gtcgatcgag aaggataccc
     1621 ggtggggtat gaagtgctgg ccggcaatgt gagagaccac aaaaccgtag cggggatgct
     1681 gcagcggctg ggagcgcggt tcggcctgac ggatcgcacg ctatgcatgg atcgggggat
     1741 ggtgaccgag gacagcctca aactgattcg agagtccacg gtccgttatg tgctggccga
     1801 tcgccggggc gcgtccgaac gatttccgga gcaagtgcag catggaccct ggcagaccaa
     1861 gcgcgccgac ccggacactg gcgaagctat ggtggaggtg caggaggtgg gtgcggaaga
     1921 aggggaccgc ttaatcctgg tacgaagccg gggatgcgcg cagaaagaga aaggcattca
     1981 cgaccgcatg ctgtccaaac tgaaagctca ctgggagcgt ctcgaaaaca cggtgagcaa
     2041 gggacggctg gtgaatccgg agaagatcga ccggcgcatc ggctcgatcc tggcgcgtca
     2101 tccggggatg tcgagttggg tgtgcgtgcg ccgcgaagaa cttcccgcgg ccac
//
LOCUS       DASRKK010000205         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1285446, whole genome shotgun sequence.
ACCESSION   DASRKK010000205 DASRKK010000000
VERSION     DASRKK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285446"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VFC15_04505"
     CDS             <1..1338
                     /locus_tag="VFC15_04505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZM09453.1"
                     /translation="KFNDKGEYLTPQGWVQPEHRKEIIRVKGKPEVTVDVEVTRHGPI
                     ISDVIPGEKRKLALKWIIYDPLPSRAAFFAVDSAKNWQEFRAAFSQFGAPGQNVVYAD
                     VDGHIGYQATGLVPIRAAGDGSVPVSGDDNSHEWTGYVPYDKLPSVYDPASGIIATAN
                     GRVTPDGYPYTLSIEWGSPYRTERIYKLLNANKKLTPPDMLAIQTDIVSAFDRFCAER
                     FVYAVDHASTASQRAKSAADLLRNWDGAMTIDSAAPTVAYFSRKKLEELLLKPKLGDD
                     WKEYRWFMSPVWLENILSHQPARWLPEGYANYDALLTAAVEGAVSDAEAPSALAMWKW
                     GRVHRVDIKHPFWSNFPILKRGAGPGPQPLSGDGQTVKQVSAHFGPSERLTVDFSDLD
                     NSTLNIVNGQSGNVFDEHYNDQWDAYYRGRTFPLPFSPSAVQRAGVHHLKLEP"
     gene            complement(1450..>2021)
                     /locus_tag="VFC15_04510"
     CDS             complement(1450..>2021)
                     /locus_tag="VFC15_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09454.1"
                     /translation="PVNAFFSADSNTAYVISCGVECGSTSGASVAQFDIPSQTIKATL
                     PVGGASVGLLNNGTLYVAGNPGPAGTYDAVNVSTMSRLTANSVAIGDGYHSTMALSAN
                     NKLYIGASTCMNITTGCLSVVDVANNTADAPLPPKGNVTGMLAIAGRNTVYVIEGGYL
                     VIYDTTTDTPQKTQVVFTGALYGVVQVDQ"
BASE COUNT          412 a          578 c          623 g          408 t
ORIGIN      
        1 aagttcaacg acaagggtga ataccttacg ccgcaaggtt gggtgcagcc ggaacatcgc
       61 aaagaaatca ttcgcgtgaa gggcaagcca gaggtaacgg tggatgtcga ggtcacccgg
      121 cacggtccaa tcattagcga cgttattccg ggagagaagc gcaagctcgc tctgaagtgg
      181 atcatctatg accccctgcc gtcacgcgct gcgttctttg ccgtggactc ggcgaagaac
      241 tggcaggaat tccgcgcggc gttctcgcag tttggtgcac cgggtcagaa tgtcgtctat
      301 gccgatgtgg atgggcatat cggttaccag gcaaccgggc tggtcccaat ccgcgcagcg
      361 ggcgacgggt cggttcccgt gtccggagat gacaactcac atgagtggac cggatacgtt
      421 ccttacgaca agcttcccag cgtgtatgac ccagcctcgg ggatcatcgc cactgccaac
      481 gggcgcgtca cgcctgacgg atatccctac acgctgagca tcgagtgggg gtcgccctac
      541 cggacagaac gcatctacaa gctgctgaat gcgaacaaga agctcactcc gcccgacatg
      601 ctggcgatcc agaccgacat tgtttcggcg ttcgatcgct tctgcgccga gcgcttcgtg
      661 tacgccgtcg accacgcatc cacggcatcg cagcgcgcca agtcggctgc cgacctcctg
      721 cggaattggg acggcgccat gaccatcgac tcggcggcgc cgacagtggc gtacttctca
      781 cgcaagaagc tggaggagtt gctgctcaag ccaaagctag gggacgattg gaaggaatac
      841 agatggttca tgagtccggt gtggctggag aacatactgt ctcatcaacc tgcgcgctgg
      901 ctgccggaag gctacgccaa ctacgacgcg ctattgacag ccgccgtgga gggggccgta
      961 agcgatgcgg aagcgcccag tgcgctcgcg atgtggaagt ggggacgggt tcatcgcgtg
     1021 gacatcaagc atccgttctg gagcaatttt cccatcctca agcgtggcgc cggtcccggc
     1081 ccgcagccgc tgtcagggga tggccaaacg gtgaagcagg tcagcgcgca cttcgggccc
     1141 tccgagcgcc ttacggttga cttctcggac ctcgacaact cgaccctgaa tattgtgaat
     1201 ggccaatccg gcaatgtctt cgatgagcac tataacgatc aatgggatgc ctattatcgg
     1261 gggcgcacgt ttccgttgcc gttctcgccg tcggcggtgc agcgagcggg cgtgcatcac
     1321 ctgaagctgg aaccgtagag gcaggggcca gggtttaggg actaggggtt agcagcgttt
     1381 acctgaacat gacccagtgg ccattgtatt agctgatagc tgatagctga cggctgaggg
     1441 atctcttccc tactggtcga cctgcaccac gccgtaaagc gctcctgtga atacgacctg
     1501 cgttttctgg ggggtgtcgg tagtagtgtc gtaaatcacc aggtatcctc cctcgatcac
     1561 atataccgtg tttcggccgg caatcgcgag catgcctgtc acattccctt tcggcggcag
     1621 cggtgcatca gcggtgttgt tggcgacatc gactacggac agacatcccg tggtgatatt
     1681 catgcaggtg ctggcgccaa tatagagctt gttgttggcg gatagcgcca tggtgctgtg
     1741 ataaccgtcg ccgatcgcaa ccgagttagc ggtcaggcgc gacatggtgc tgacgttcac
     1801 cgcgtcatag gtcccagcgg ggccgggatt tccggctacg tacaaagttc cattgttgag
     1861 caggccaaca ctggcgcctc cgaccggcaa ggtcgccttg atggtttgcg acggaatgtc
     1921 gaactgcgct acggatgcac ccgaagtcga gccgcactca actccgcaac tgatcacata
     1981 ggcggtattg ctgtcggcgc tgaagaacgc attgaccggc c
//
LOCUS       DASRKK010000206         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1285464, whole genome shotgun sequence.
ACCESSION   DASRKK010000206 DASRKK010000000
VERSION     DASRKK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285464"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="purB"
                     /locus_tag="VFC15_04515"
     CDS             complement(<1..869)
                     /gene="purB"
                     /locus_tag="VFC15_04515"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZM09455.1"
                     /translation="MIARYTRPEMGHIWSDENKFRTWLEVEVAATETLAEAGIVPQAA
                     AKAIRERGGFDLARINQIEAEVKHDVIAFTTTVAEKVGPESRWLHYGLTSNDVVDTAQ
                     ALLMKQASGIIREDLKKLREVLKRRAWEFKDTPAIGRTHGIHAEPITFGLKLANWYSE
                     MQRNIERFEMAAEQMRVGKLSGAVGNAAHLEPEYEEKICQRLGLNVAAVSSQVIQRDR
                     HAFYVATLAVIASTLDKIATEIRHLQRTEVREAEEYFSEKQKGSSAMPHKRNPVTCEQ
                     ISGLARVVRANSQA"
     gene            996..1319
                     /locus_tag="VFC15_04520"
     CDS             996..1319
                     /locus_tag="VFC15_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZM09456.1"
                     /translation="MRNTIARNFTALTAGPLPFERRRGNRILVHMAVRISGQGLLDKV
                     SQDGTCTDISEAGIGFETDADLYVGEIVDLEFPRQDGDPFRFQARLLYKMGNRYGAYL
                     VSPGS"
     gene            complement(1578..1838)
                     /locus_tag="VFC15_04525"
     CDS             complement(1578..1838)
                     /locus_tag="VFC15_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZM09457.1"
                     /translation="MALIVYSSSWCPDCRTAKRFLDKHNISYTDIDIEATPGAADEVI
                     RHAGKRAVPQFVIDGRWVQPYRPGEGFLYEEMSELLGIMDEG"
     gene            complement(1971..>2466)
                     /locus_tag="VFC15_04530"
     CDS             complement(1971..>2466)
                     /locus_tag="VFC15_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09458.1"
                     /translation="SNIGARHLQKRTGMGFQSEHEETISTKVEDMVKSEVKRTFNPEF
                     LNRLDEVIIFNALSEADLIQIVELMVQQLNQNLAQRAITVTVAEEARKWILDQTIVDR
                     SYGARPLRRALQKYIEDPLSEALIQGTFAQRPTFIEVYLETNQLFYRPVGEEKTEGVL
                     LYSN"
BASE COUNT          487 a          746 c          702 g          531 t
ORIGIN      
        1 gcctgcgagt tcgcacgcac cacgcgcgct aggccgctga tctgctcgca ggtgaccggg
       61 ttgcgcttgt gcggcatggc cgacgagccc ttctgcttct cgctgaaata ttcttccgcc
      121 tcgcgcactt cggtgcgttg cagatggcga atctcggtgg cgattttgtc cagcgttgag
      181 gcaatgaccg ccagggttgc aacgtaaaac gcgtggcgat cgcgctgaat cacctgcgag
      241 gaaactgccg cgacgttcag tcccaggcgc tggcagatct tctcttcgta ttccggttcg
      301 aggtgcgccg cgtttcccac cgctccggag agtttgccca cgcgcatctg ctcggcagcc
      361 atctcaaagc gctcaatatt gcgctgcatc tccgaatacc agttcgccag cttcagccca
      421 aacgtaatcg gctcggcgtg gatgccgtga gtgcggccga tcgccggcgt gtccttgaac
      481 tcccacgcgc ggcgcttcaa cacctcgcgc agcttcttca agtcctcgcg gataatgccc
      541 gacgcctgct tcatcagcaa cgcttgcgcg gtgtccacca catcgttcga agtcagaccg
      601 tagtgcagcc agcgcgattc cggaccgacc ttttccgcca cggtggtcgt gaaggcgatg
      661 acgtcgtgct tcacttcggc ttcgatctgg ttgatgcgcg ccaggtcgaa accgcccctt
      721 tcgcgaatgg ccttggctgc cgcctgcgga acgattccgg cctcggccag cgtttccgtg
      781 gcggcgactt ccacctccag ccacgtccgg aacttgtttt cgtcactcca gatgtgcccc
      841 atttcgggac gggtataacg agcgatcaag cgagctcctt cacagccagc aatgttggac
      901 agaagaaaac tctttattgt aactgctggc acagggtgcg tttcagtttt cgaacaaaac
      961 taaaggctgc cggagatctt gccgataact tcgggatgag gaacacgatc gcgcgaaact
     1021 ttacggcgct gacagccgga cctcttccgt tcgagcggcg caggggaaac cgcatactgg
     1081 tccacatggc cgtgcggatc agcggccagg gtttgctgga taaggtttct caagatggga
     1141 cctgcaccga catcagcgaa gccggcatcg gtttcgagac cgatgctgat ttgtatgttg
     1201 gggaaatcgt tgacctggaa ttccctcgcc aggacgggga ccccttccgc tttcaggcaa
     1261 ggctgctcta taagatgggg aaccgctatg gcgcctatct tgtatcgcct ggctcctgat
     1321 ccaacaggaa tcgaaaaaag cgcgggccac gtgccccatt cttgggcagt gggatgagat
     1381 acccatccgc tagagcgggg aacagacatc ccaacaccat cttcccaaca tatatcgtaa
     1441 tacgatgata tcgtattcat ctgccatctc agcccccggt tctcacacac gctctaaagc
     1501 ccaactcttc tctagcccgc acggcacagc aaaagctgta ccctttcgag acaaaaagac
     1561 aattggaact gcaccgatca gccctcgtcc attattccca gaagctcact catttcttcg
     1621 tacagaaacc cttcgcccgg acgatagggc tgcacccagc ggccatcgat gacgaactgc
     1681 gggacggcgc gcttgccggc gtgacgaatg acctcgtcag cggcgccggg cgtggcttca
     1741 atatcgatgt cggtgtagga aatgttgtgc ttgtcgagga agcgcttggc ggtgcggcaa
     1801 tcggggcacc atgaagacga ataaacgatc aaagccatat tgcaattgga tgaaaatcgc
     1861 gggtccaggc tgcaggaagt gggcggtacc tcggggaggc aatggagacg gagcaaaaca
     1921 agaggggaag cgtttccgct cccccttttg ctttgcacac tgctctgact ctagttgcta
     1981 tacagcagca cgccctcggt cttctcctcg ccgacagggc gatagaagag ctggttggtc
     2041 tccagataca cctcgatgaa cgtgggccgc tgggcgaacg tgccctggat gagcgcctcc
     2101 gacagcgggt cctcgatgta cttctgcagc gcccggcgca acggacgcgc accgtagctg
     2161 cggtccacga tcgtctggtc gaggatccac ttgcgggcct cttccgccac cgtgaccgtg
     2221 atggcgcgct gcgccaggtt ctggttcaat tgctgcacca tcaactccac gatctggatt
     2281 aggtctgcct cgctaagcgc gttgaagatg atgacctcgt cgagacggtt gaggaattcg
     2341 gggttgaagg tgcgcttcac ctcgctcttc accatgtcct cgaccttggt ggagatcgtt
     2401 tcttcgtgct ccgactggaa gcccatgccg gtgcgcttct gcaagtggcg cgcgccgatg
     2461 ttcgag
//
LOCUS       DASRKK010000207         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1285482, whole genome shotgun sequence.
ACCESSION   DASRKK010000207 DASRKK010000000
VERSION     DASRKK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285482"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..2100
                     /locus_tag="VFC15_04535"
     CDS             502..2100
                     /locus_tag="VFC15_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM09459.1"
                     /translation="MDNEWIDGKVKQRVLFRLGRLDELLANGRLDSLVRSMGRFSEKL
                     AILGAHARGQSVATRSARIGPALIFQRLWQACSIGPVLTALLKDRRFEFSVERAIFLT
                     VLHRLFAPGSDRAAEKWKDDYSIEGVGELGLHHLYRAMAWLGEVLPKDRQNGATPFAP
                     RTNKDLIEEDLFARHRDLFSGLDLVFFDTTSIYFQGEGGETIGKRGHSKDHRPDLRQM
                     VVGIVLDRNGNPVCSELWPGNTADVTSLVPIVERLKSRFGIGSVCVVADRGMISAATL
                     AEIEKREWQYILGVRMRSSNEAKAVLARAGRYAQVHPKSADPDDPSPLKVKEVWMEDT
                     RRYVVCVNEDQATKDRFDREAVVSSLRDALGNGDKSLVGNKGYRKFLRAGGKRFAVDE
                     EKIREEARYDGKWVLTTNTDLSTREVALKYKQLWMVEDVFRSMKSLLDTRPIFHKCDE
                     TIRGHVFCSFLALLLRKELEDRLASKEWKLEWADVIRDLDNLVEMEVAINGKGYVIRG
                     QASGVAGKVFQACGVALPPTLRSC"
     gene            2341..>2692
                     /locus_tag="VFC15_04540"
     CDS             2341..>2692
                     /locus_tag="VFC15_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09460.1"
                     /translation="MAQTLEVFLAAESGDAALVIVKQRRAKYISTKKRVPNSSRRSHV
                     LLASGQDFVKGFAGPTKRVCVKVPFPHKRIQAFSQMFLVYEIDDAESLALQNAKPLFH
                     LVHPRAMHGRVMELE"
BASE COUNT          573 a          712 c          835 g          572 t
ORIGIN      
        1 gtcgcattca ctgccaatcc gaccagcgtt atacaggcca ggtatgaaca agtagccaat
       61 tggaccgcat ccgcagagag cagtgttgtt acttccgcat gcttcgcgac gttttagcca
      121 agccaggatc ggcatagtga aaaaagagcc gctaccgtga tggctatgcc gagcatgttt
      181 gtctcaacgt ggcgcaggcg agcacctccg tgggcgtggt cggtgagatg aaattgagag
      241 ggctggcgct atctctttga atgcaagatc tcttgccatg cccaaactca tcttcaacac
      301 tttgcgcaat aaagtttcca taagttattg attccactga cggctatcgc ctcgggcgtg
      361 aattcctagt gtcacgtttg tggtagataa tctcatatgt tccttgttat ttccggaatc
      421 ttgtggtcat gatagtgaca caggagcaat actctgttcg ttcgcactca gaccaacggt
      481 tccagaacct atctgctcat cgtggataac gagtggatcg acggcaaggt caaacagcgc
      541 gttttgtttc gcctcggccg cctcgatgaa cttctcgcca acggccggct cgactctctc
      601 gtccggtcta tgggacgatt ttcggagaaa ctcgccatcc tcggcgcgca tgctcgcggc
      661 cagagtgtcg ccactcgcag cgcccggatc ggtccggcgc tgatcttcca gcgactctgg
      721 caagcctgct ccatcggccc ggtgttgact gctttgctga aggaccgccg tttcgagttt
      781 tccgtcgagc gcgccatctt tctcaccgtg ctgcatcggc tcttcgcgcc gggcagcgac
      841 cgcgccgccg aaaagtggaa agacgactac tccatcgaag gcgtcggcga gttgggtctg
      901 caccatctct accgagctat ggcttggctg ggagaggttc tgccgaaaga ccgacagaac
      961 ggggctacgc ctttcgcgcc gcgtaccaac aaggatctca tcgaggagga cctgttcgcg
     1021 cggcatcgag acttgttctc cggtttggat ctggtgttct tcgataccac ttcgatctat
     1081 ttccagggcg agggcggcga gaccatcggc aagcgtgggc actcgaagga ccatcgtccc
     1141 gatttgaggc aaatggtggt gggcatcgtg ctggatcgga acggcaatcc cgtctgcagc
     1201 gaactctggc caggcaacac agccgacgtg acgagtctgg ttcccatcgt ggaacggctg
     1261 aagagccggt tcggcatcgg ctcggtttgc gtcgtcgccg atcggggcat gatcagcgcc
     1321 gcgactctgg cggaaatcga gaagcgggag tggcagtaca ttttgggcgt gcgcatgcgg
     1381 agttccaacg aagccaaagc ggtgctggcg cgcgccgggc gctatgcgca agtgcatccc
     1441 aaaagcgcgg acccggacga tccatcgccg ctgaaggtga aagaagtctg gatggaggac
     1501 acgcgccggt atgtggtgtg cgtgaacgag gatcaagcca ccaaggatcg cttcgaccgc
     1561 gaggctgtgg ttagctcgtt gcgggacgcg ttggggaacg gagacaagtc gctggtgggc
     1621 aacaaggggt accgcaagtt tctgcgggcc ggcggaaagc ggttcgccgt ggacgaggag
     1681 aaaatccggg aagaagcgcg ctacgacggc aagtgggtgc tgacgacgaa cacggatctg
     1741 tcgacgcgcg aggtggcgct gaagtacaag caactgtgga tggtggagga tgtgttccgg
     1801 tcgatgaagt cgctgctgga tacccggccg atctttcaca aatgcgacga gacgatccgc
     1861 gggcatgtct tttgttcgtt cctggcgctc ctgttacgca aggaactgga agaccgattg
     1921 gcatccaagg agtggaaact ggagtgggcc gacgtcatcc gagatctgga caacctggtt
     1981 gagatggagg ttgcgatcaa cggcaaaggc tatgtaattc gcggccaagc gtcgggagtg
     2041 gcggggaagg tctttcaggc ttgcggtgtc gccttgccgc ccacgttgcg ctcatgctga
     2101 ctcagatcag ctatgtgccc gggaaagagg gcgtgtcact aggtcgtttt cgaaatcgta
     2161 acccattgag ggcggccaac ttgcagcatc gtggtgtcga agatcagctt gagactggaa
     2221 ccaagttctg acagattcgt cccactggac ggaagcggga atccggaggg gctgcgacgg
     2281 actgagattc cgatgctttc gagaatgggg ttgtgaggcc cgaaccccag aatcatgctt
     2341 ttggcgcaaa ctctcgaagt gttcttggcc gccgagagcg gggacgccgc tcttgtcatc
     2401 gtaaaacaaa ggcgcgctaa gtacatctca acgaagaaaa gggtgccgaa ttcaagccgc
     2461 cgcagccatg ttctgctcgc atctggccag gactttgtca aaggatttgc gggaccaacg
     2521 aaacgagtgt gcgtgaaggt tccattcccg cacaaacgca tccaggcgtt cagccagatg
     2581 tttcttgtct acgaaatcga cgatgccgaa tcgcttgcgc tgcagaatgc taaaccactg
     2641 ttccacttgg ttcatccaag agcaatgcac gggagggtga tggaactgga ag
//
LOCUS       DASRKK010000208         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_699244, whole genome shotgun sequence.
ACCESSION   DASRKK010000208 DASRKK010000000
VERSION     DASRKK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699244"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VFC15_04545"
     CDS             <1..1183
                     /locus_tag="VFC15_04545"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM09461.1"
                     /translation="KALVRIVGQAPLAATVYSLERLRCGACGQVFTAQEPEGVGPEKY
                     DETAAAMIAQLKYGSGVPFYRLEQLEDQLGIPLPAATQWEIVEEQAELIEPARDELIR
                     QAAQGEVVHNDDTGMRVLCLAREASDERTGVFTSGIVSTGQGWKIALYFTGRQHAGEN
                     LADVLKQRAKQSSPPIQMCDALSRNVPKLSAGAEILLANCLAHGRRQFVEVAANFPDE
                     CRYVLELLGQVYGYDAEARERGLASEQRLRFHQEFSTPVMDQLHGWLEAQLAERKTEP
                     NSGLGKAITYLLRHWRPLTTFLRVAGAPLDNNICERALKRAVLHRKNALFYKTLNGAQ
                     VGDLFMSLIHTCQLCGANSFDYLIELQRHAQELAASPAQWMPWNYRETLAQTVSLPDS
                     S"
     gene            1228..1908
                     /locus_tag="VFC15_04550"
     CDS             1228..1908
                     /locus_tag="VFC15_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM09462.1"
                     /translation="MRIALFEMAEKRASEKAKSANVFGQARKQPKMFRAGLYARVSTT
                     DQQTIPLQIRALREYTARRGWTIALQVKEVGSGTSQRELRERVLDAARRREIDVVLVW
                     RLDRWGRSVTDLLTTLQELDHLGVSFVSLTEALDLTTPTGRAMAGLLAVFAAFEREIL
                     GERVRAGLAHARQSGKRLGRPVTAAIHAGQMRKLFRNGVSKAEIARRLQIGRTSVRRI
                     LAAGSTKK"
BASE COUNT          466 a          624 c          717 g          402 t
ORIGIN      
        1 aaaggcgttg gtgcgaattg tgggccaggc gccgctggcg gccaccgtct actcgcttga
       61 gcgcttgcgc tgtggcgcgt gcgggcaggt gttcacggca caagagccgg aaggagtagg
      121 acccgaaaag tacgacgaga cggcggcggc gatgatcgcg caactcaagt acggcagcgg
      181 cgtgccgttc taccggttgg agcagctcga agatcagctc ggcattccgc taccggcagc
      241 aacccaatgg gaaattgtgg aagagcaggc ggaattgatc gagccggcgc gcgatgagtt
      301 gatccggcaa gcggcgcagg gcgaggtggt gcacaacgac gacacgggca tgcgagtgtt
      361 atgcctcgcg cgggaggcgt cggacgaacg gacgggcgtg ttcaccagcg gcatcgtgtc
      421 gaccgggcag ggctggaaga tcgcgctgta cttcaccgga cgccagcatg ccggggagaa
      481 cctcgccgac gttttgaagc agcgggccaa gcagtcgagc ccacccattc agatgtgcga
      541 tgcgctgtcg cgcaacgttc caaagttatc cgcgggtgcg gagattctgc tggccaactg
      601 cctcgctcac ggaagacgtc agtttgtgga agttgcggcg aacttccccg acgaatgtcg
      661 ctacgtgctc gagttgctgg gccaggtgta tggctatgat gccgaagcgc gtgagcgcgg
      721 cctggcttcg gaacagcggc tgagattcca tcaggaattt agcactccgg tgatggacca
      781 actgcacggc tggctggagg cgcagcttgc cgaacgcaag acggagccaa attccgggct
      841 gggtaaggca atcacgtatc tactgcggca ctggcgtcct ttgacgacgt ttctccgtgt
      901 ggccggggca ccattggaca acaatatttg cgaaagggcg ctgaagcggg ccgttctgca
      961 caggaagaac gccttgttct ataagacgct gaacggagcg caggtcggtg atctgtttat
     1021 gagcttgatc cacacctgcc aattgtgcgg cgccaattcc ttcgactacc tgatcgagct
     1081 gcaacggcac gcccaggagt tagcggccag ccccgcgcag tggatgccgt ggaactaccg
     1141 cgaaacgctc gcacagaccg tgtccctccc ggattcctcg tagagttatg atggatctcg
     1201 cgtggccgaa aaggatagtc ttcggccatg cggatcgcac tcttcgaaat ggccgaaaag
     1261 cgggcatctg agaaggcaaa atccgccaac gtttttggcc aggcacggaa acagccgaaa
     1321 atgtttcgcg ccggcctcta cgcccgcgtc tccaccaccg accaacagac catcccgttg
     1381 cagattcgcg ccctgcggga gtacaccgcc cggcggggct ggactatagc cctgcaggtg
     1441 aaagaggtcg gctccggcac gtcgcagcgt gaactgcgcg aaagagtgct cgatgctgca
     1501 cgtcgccgcg agatcgacgt ggtgctcgtc tggcgactgg atcgatgggg ccggtctgtg
     1561 acggacctgc tgacgacact ccaggaactg gaccacctcg gcgtaagttt cgtctcactg
     1621 acagaggccc tggacctgac tacgccgacc ggtcgagcca tggccggcct tctggccgtc
     1681 ttcgcggcct tcgaacggga aatccttgga gagcgagtgc gggctggtct ggctcacgcc
     1741 cgccaaagcg gcaagcgact ggggcggcca gttacagcag caatccacgc cggtcagatg
     1801 cggaaattgt tccgcaacgg cgtcagcaag gcagaaatcg cccgccggtt gcagatcggg
     1861 cgcacctcgg tacgccgcat tctcgccgcc ggctcaacaa aaaagtaggg agacgatacc
     1921 ttgcgcagct ttaccgaaag gacacgctgt actgaaagcc cggacaaagt tccggctgaa
     1981 gatcgtccgg gatttaacta caaatgatgg cgacaatagg aagcttcctc gcacagtgat
     2041 gcgtttaaga gcaactcggt tcgtcctgga tttgaatgca gtatgctcgt gcgagtccgg
     2101 gatttgagtg caggaaacag tgttgttggc tctcagtccg ggatttgagt actgtgcgac
     2161 aactggggac cgcaactgga cggcggcatg aagacgaata ctctgaggg
//
LOCUS       DASRKK010000209         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_601253, whole genome shotgun sequence.
ACCESSION   DASRKK010000209 DASRKK010000000
VERSION     DASRKK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601253"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFC15_04555"
     CDS             <1..532
                     /locus_tag="VFC15_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM09463.1"
                     /translation="LSGRIQISPDIDYLERAFDASKYGQFSRHPYLEATIPTLWDESL
                     APAGKHVMSIYMQYAPFHLRNADWDSQRDALGDVVVKTLAEYAPDLAGLVEAREVITP
                     KDLEQTYGLTGGHIYHGELALDQLFTMRPLLNWAQYRTPIKRLYLCGSGTHPGIGLTG
                     GSGANAAREIIKDKHK"
     gene            complement(699..1802)
                     /locus_tag="VFC15_04560"
     CDS             complement(699..1802)
                     /locus_tag="VFC15_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZM09464.1"
                     /translation="MDSSLHDILASYNDRSPLEQSYTIPASWYVDGRVAELERQNVFG
                     RTWQVVARVDQLQQPGQYVTSDLAGEPLVIVRGNDQQLRAFYNVCRHHAAAVVTEEQG
                     TASILRCPYHGWSYGLDGSLKGAPEFEGVCSFDRAQNGLLPVRVETWEQFVFVNLDPK
                     AAPLAEFLGRLAQRVAPLDVGSVHFFERRSYSLNCNWKVFVDNYLDGGYHVPHLHKAL
                     NSVLEYKQYTIENEDRYCLQSSPMVASNEDAATSATRTGDRARYFWQYPNFMINLYQG
                     YMDTNLVLPDGVDRCKVIFDFYFDDVSEPAAERNRQSIAVGERLNDEDVGICEAVQRG
                     LHSRAYGAGRLSVRREAGEHLFHRLLAADLKRG"
     gene            2230..2691
                     /locus_tag="VFC15_04565"
     CDS             2230..2691
                     /locus_tag="VFC15_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM09465.1"
                     /translation="MRFPTESLEVVQATPADVGEVAPLFDAYRQFYKKPPDLEAARRF
                     LFARLSKGESVLFLARHNARTVGFVHLYPVFSSVNLTRQWILNDLYVAVEARKLGVGR
                     ALMKRAHQLAEDTQADGLTLETAIDNHASQKLYESLGWKRDEEFYRYCLPL"
BASE COUNT          592 a          853 c          857 g          578 t
ORIGIN      
        1 tttgtctggc aggatccaga tctcgcccga catcgactac ctggaacgcg ctttcgacgc
       61 ctcgaagtat ggacagtttt cacggcaccc ctacctggag gcgaccattc ccacattgtg
      121 ggacgaatcg ctcgctccgg ccgggaagca tgtgatgtcc atctacatgc aatatgcgcc
      181 gttccatctg cgcaacgccg actgggactc gcaacgcgat gctctgggcg acgtggtggt
      241 gaagaccctc gcagaatacg ctcccgattt ggcggggctg gtcgaggctc gcgaggtgat
      301 cacgccgaag gaccttgagc aaacctatgg cctgacgggc gggcacatct atcacggaga
      361 gttggcgctc gatcagttgt tcaccatgcg gccgctgctg aactgggcgc agtaccgaac
      421 gccgatcaag cgcctctatt tatgcggctc gggtacgcat cccgggattg ggttgacagg
      481 cgggtcgggc gcgaacgctg ctagagagat aattaaggat aaacacaagt gatacaaaaa
      541 ccactcaacc tcggatttcc acggatcttc acggatgtcg tcgaccgtaa ggttgccttc
      601 tgtaatccgg ttcgctgaga ggtctcgttc gaagcacctc ttatccgtgg agatccgtgt
      661 caatccgtgg ttgttgtcac cacacaattg gcgagatcct accctctctt caagtccgcc
      721 gccaacagcc gatgaaaaag atgctcgccg gcctcccgcc gcaccgataa gcgccctgct
      781 ccgtaggctc gcgagtgtaa tccacgctgt acggcctcgc agatgcccac gtcttcgtca
      841 ttcaggcgct cgccgacggc gatactctgc cggttgcgct cggcggcggg ctcgctcacg
      901 tcgtcgaagt agaaatcgaa aatgacctta cagcgatcaa cgccatcggg cagcacgagg
      961 ttcgtatcca tgtagccctg gtagagatta atcatgaaat tgggatactg ccagaagtac
     1021 cgggcgcggt cgccagtgcg ggtggccgag gtagcagcgt cttcgttcga ggcgaccatc
     1081 ggactcgatt gcaggcagta tcggtcttcg ttctcgatag tgtactgctt gtactcgagc
     1141 acgctgttca gcgccttgtg caggtgcggc acgtggtagc caccatcgag gtagttatcc
     1201 acgaacactt tccagttgca gttgagactg taagatcggc gctcgaagaa atgaactgag
     1261 ccgacatcga gcggcgcaac tcgctgcgcc agcctgccca ggaattccgc taggggcgcg
     1321 gctttcggat ccaggttcac gaagacgaac tgctcccagg tttccacgcg cacggggagc
     1381 agaccattct gcgcgcgatc aaacgagcag acgccttcaa actccggcgc acctttcagc
     1441 gagccgtcaa gaccatagct ccaaccatga tagggacagc gcagaatgct tgccgtacct
     1501 tgctcctcgg tcacgacggc agcggcgtgg tgccggcaga cgttgtagaa cgcacgcaac
     1561 tgttggtcgt tgccgcgcac gatgaccagc ggttcgccgg cgagatcgct ggtgacgtac
     1621 tggccgggct gctgcaactg atccacgcgc gccactactt gccacgtgcg gccaaagaca
     1681 ttctggcgct ccagttcggc gacgcgcccg tccacgtacc acgaagccgg aatggtgtag
     1741 gactgctcca gtgggctccg gtcgttgtag gaggcgagaa tgtcgtgcag cgaagagtcc
     1801 atctacaaag gctccgaatc gaaagcgtta cctcagcggg taaagccgac cgtaactagc
     1861 acagtttacg gcacgcgtga aggcgtgcgg aaaaaagaga tggtcaggta gtgcggcgct
     1921 tcagcgccgc gtcaacgatc tgatcagaaa gtggccttta ggcccgggta catgctccga
     1981 tgtcccgaac gcgcgtgttt tcccctgagt ttcaaaacga agtacctcag cggctaaagc
     2041 cggctcatag atcaccgctc gacgcgagcc tgaaggcccg cactacccgg gcagatttga
     2101 gcatttttcc gcaacctgtg aaggctcgcc cttcaaagca gcgccgctga actggttttt
     2161 cgagatgata gcaggggact gcgcaacaaa agagttgacg gccagccgcc gtcgtgtttc
     2221 cttgtcccca tgcgtttccc aacagagtct ctggaagttg tgcaggccac gccggccgat
     2281 gtcggcgaag tggcgccgct cttcgatgcc taccggcagt tctacaagaa gcctcccgac
     2341 ctggaagcgg cgcgccgctt cctgttcgca cgattgagca aaggcgagtc ggtgctgttc
     2401 ctcgcccgtc acaacgcgcg gacggtcggc tttgtgcatc tttatcccgt gttctcgtcg
     2461 gttaacttga cgcggcaatg gatcctcaac gacctctacg tggctgtgga ggcgcgcaag
     2521 cttggcgtgg gacgggccct gatgaagcgc gctcatcaac tggcagagga cacgcaagcc
     2581 gacggcctga ccttggagac ggcgattgac aatcatgcct cgcaaaagct gtacgaaagt
     2641 ttgggctgga agcgcgacga ggagttctat cggtattgtc tgccgttgtg acagccggaa
     2701 tttcatgcac tagccagcaa tgaagatcga cccgagcgcc aatcgagcag ccgccgagcc
     2761 tgacacttcc ccgcaaccca agcgggtcct gggtttccgc gatttggtga tgttctacgt
     2821 ggtcgccacg ctgagcctgc gctggattgc ggtggcggcc agcgcgggac cgagcacggt
//
LOCUS       DASRKK010000210         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_308139, whole genome shotgun sequence.
ACCESSION   DASRKK010000210 DASRKK010000000
VERSION     DASRKK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308139"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFC15_04570"
     CDS             complement(<1..272)
                     /locus_tag="VFC15_04570"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZM09466.1"
                     /translation="MNIYLDVQSPAVKEAGKLASAYGLANHGLVNLRRAYWNLPTPAL
                     YEESIFRSEGSLSHLGPLVVSTGKHTARAAADKFIVREQTTEDKIW"
     gene            complement(269..1486)
                     /locus_tag="VFC15_04575"
     CDS             complement(269..1486)
                     /locus_tag="VFC15_04575"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM09467.1"
                     /translation="MSLSQLARSIRESATLKLNETAALLRDKGEPIIHLGGGEPKSKA
                     PIDAVINCAALLNTGEIRYTAPDGIPALKKGIIRYTEEHYKKIVSADNVIASGGAKQA
                     LMVLLHALLDPKEEVIFPAPYWVSYPEMVKLAGGVPVRVAAEDGSFCPTVKEMADACG
                     SYTKAIILNSPNNPSGAMYSNDFVQEMVEFCERRSIYLIMDDIYNRLVFDGKSAPIAY
                     DFMKSPLEQSKLVVINGVSKMYAMTGFRIGWAVANKPLIEAMATIQSQQTSGPASPSQ
                     WAAVGALNGVQSSVENLRVTLENNRNVMMDRLHAFDGVKIHKPEGTFYCFPDFSAYMK
                     DSQKLSHFLLEKVRVVTVPGKEFGFEGHLRLSYCGTIRDVTEGLDRIKWALDPNSSNE
                     LYLGDRKLVRDWL"
     gene            complement(1827..2366)
                     /locus_tag="VFC15_04580"
     CDS             complement(1827..2366)
                     /locus_tag="VFC15_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09468.1"
                     /translation="MGLGTAAFWVVLNADSAAKLMPWLVFVPDKATCCGLPPPLSLTE
                     TAPLKVPRLCELKRTVMVQDPLGCTLDPQLLVSESSCCRGQTEQPVETQFTLSDQWSR
                     RLFTALCRRYGLEPYRYRRQRHTTVVVRAPRSFINNTLWPEFLQIKDALDEYLNEATE
                     RIIREEVYGNSAEAPERAE"
     gene            2400..3008
                     /locus_tag="VFC15_04585"
     CDS             2400..3008
                     /locus_tag="VFC15_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09469.1"
                     /translation="MVDPLRDDPFLGNGTKRELLVRFWYPASITEGCDPAAYTSPAVW
                     SYFSELAGFPLPDVRTNSCLDAPISMGPHPVVVFTHGYTGTFTDYTFLFEDLASRGYV
                     VAAVDHTYEATAVEFLDGRLVKSVFGSHLAENTLRRDGQSFSLAVSMRSKDLQFVVDE
                     LERLNGEASGRSARLPHPSWQTCWSGHDRVAYSPPHLSLMDG"
     gene            complement(2988..>3230)
                     /locus_tag="VFC15_04590"
     CDS             complement(2988..>3230)
                     /locus_tag="VFC15_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09470.1"
                     /translation="FESLSLRHVFNNLRGVTVKLRSNNRDRDYWITILTTALFASRIA
                     SGMALPYSFMVILMSVWRIKSFWIPSGVPLLSIHER"
BASE COUNT          688 a          919 c          923 g          700 t
ORIGIN      
        1 caccagatct tgtcttctgt ggtttgctcg cgcacgatga atttgtcagc cgcggcacga
       61 gcagtatgtt tgccggtgct caccaccagc ggtcccaggt gcgagagact gccctcggaa
      121 cggaaaatgc tctcctcata aagcgccggc gtcggcagat tccagtaggc acgccgcaga
      181 ttcaccagcc catggttggc gaggccgtag gcgctggcca acttccccgc ctcttttaca
      241 gcgggagatt gcacgtcgag gtagatgttc atagccaatc cctcacaagc ttgcggtcgc
      301 cgaggtagag ttcgttggac gaattcgggt ccagtgccca cttgatgcga tccagaccct
      361 cggtgacatc gcggatggtg ccgcagtaac tcagccgcag atgcccctcg aagccgaatt
      421 ccttgcccgg caccgtgacc acccgtactt tctccagcag gaaatgggaa agcttctgcg
      481 aatccttcat gtatgcgctg aaatccggga agcagtaaaa ggtgccttcc ggcttgtgga
      541 tcttaacgcc gtcaaaagca tgcaggcggt ccatcattac gttgcggtta ttctccagcg
      601 tgacccgcag gttctcgacc gacgattgca cgccgttgag cgcgcccacg gctgcccact
      661 gcgagggcga agccggaccg gaggtctgtt gtgactggat cgtcgccatg gcttcgatca
      721 gcggcttgtt cgcgactgcc cacccaatcc ggaagccggt catggcatac atcttcgata
      781 ccccgttgat gaccaccagc ttcgattgtt cgagcggcga cttcatgaag tcgtaggcga
      841 tgggagcgct cttaccgtcg aacaccagtc ggttgtagat gtcgtccatg atgagataaa
      901 tgcttcgcct ctcgcaaaac tcgaccatct cctgcacaaa atcatttgag tacatggcgc
      961 ccgaagggtt attcgggctg ttcagaatga tggccttggt gtaggacccg cacgcatcgg
     1021 ccatctcttt aacagttgga caaaacgatc cgtcctcggc ggcgacccgc acgggcacgc
     1081 cgcccgcgag tttcaccatc tccggatagc tcacccagta aggggccggg aaaatcactt
     1141 cttccttggg atcgagaagg gcgtgcaaca gcaccatgag tgcctgcttg gcaccccccg
     1201 aagcgatcac gttatcggcc gacacaatct ttttgtagtg ctcttcggtg tagcgaatga
     1261 tgcctttctt gagtgcagga atcccatcgg gcgccgtgta ccggatttct cctgtgttca
     1321 gcagtgccgc gcaattgatg acagcatcaa tgggcgcctt actcttcggt tcgccgcctc
     1381 ccaggtgaat gattggctcg cctttgtcgc gcagcagagc cgcggtttca ttcagcttga
     1441 gagtcgcaga ttcccggatg gaccgggcaa gctgactaag actcatgatg tgtggcccct
     1501 ttctcgggcg tggttgacct gtcacttcta tgttggcgaa agtgggcgtg actgggcatt
     1561 tgatatcaag tagattctga aactgaaagc tcgcacggtg atctccatca cggaattaag
     1621 tgactcgccg gagacgtcac cagcagcgtt ctgattggcg acgagacgtc gctgagccta
     1681 gcaccgcttc cgcccgagcg acggacaaac cggagattga ccgaatcagg gaagcgctgc
     1741 ggacaatcgt gttccacgag ggaaccactg aagaagtgtc gaaagcaatg gatgatgtca
     1801 aaaccgcgag gtggtaaacg cgactctcac tctgcccgtt ccggagcttc agcggaattg
     1861 ccgtagacct cttctcgaat gatcctttcc gtagcttcgt tcaagtattc atcgagcgcg
     1921 tccttgatct gcaaaaattc cggccagaga gtgttattga tgaacgatct tggagcccgc
     1981 acaacgaccg tcgtgtggcg ctggcgccgg tagcgatagg gttcaagacc gtagcggcga
     2041 cacagcgcgg tgaacaggcg ccggctccat tgatctgaga gcgtgaattg cgtttcgacc
     2101 ggctgttcag tttgcccgcg acagcaagag ctctccgaga ccaacaattg cggatcgagc
     2161 gtgcagccca gagggtcctg caccatcacg gtgcgcttca actcacaaag cctcgggacc
     2221 ttcaacggcg ctgtctccgt gagcgacaac ggcggtggca gtccacagca ggtggctttg
     2281 tcagggacga aaaccagcca cggcatgagc ttagcggcgc tgtcggcgtt aagcacgacc
     2341 cagaaggcag ccgttcccag ccccacgggg cccagccaag tcggtacacg cgtcgtgcga
     2401 tggttgaccc cttacgagac gatccttttc tgggcaatgg aaccaagcga gaactgctgg
     2461 tccggttctg gtatccagcg tctatcactg agggctgcga cccggcggca tatacctcgc
     2521 cggcggtttg gagctatttt tctgaactcg cgggattccc gttgcccgat gtcaggacca
     2581 acagttgtct ggatgctccc atcagtatgg ggccgcatcc cgtagttgtt ttcacacacg
     2641 gttacacggg tacgttcaca gattacacat tcctctttga agacctcgcc agccgtggtt
     2701 acgtggttgc cgcagtggac cacacctacg aggccaccgc cgtcgagttt ctcgatggac
     2761 ggttagtgaa gtcggttttt ggcagccatc tcgcggaaaa cacattgcgg agagatggac
     2821 aaagcttttc tttagccgtg tccatgcggt caaaggacct gcaattcgtc gttgatgaac
     2881 tggagcgcct gaacggcgaa gccagcggcc gttcagcgcg actacctcat cccagctggc
     2941 aaacgtgctg gtctgggcat gatcgggtgg catactctcc gccacaccta tcgctcatgg
     3001 atggataaca acggcacgcc gcttggaatc cagaaagact tgatgcgcca cacggacatc
     3061 aagatgacca tgaatgagta tggaagtgcc atgccggagg cgatgcgtga ggcaaacagt
     3121 gcagtagtaa ggatggttat ccagtaatcc cggtcccggt tatttgaccg tagcttgacc
     3181 gtgacgccac gtaagttatt gaaaacatgg cggagagaca gggattcgaa
//
LOCUS       DASRKK010000211         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_699558, whole genome shotgun sequence.
ACCESSION   DASRKK010000211 DASRKK010000000
VERSION     DASRKK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699558"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFC15_04595"
     CDS             complement(<1..642)
                     /locus_tag="VFC15_04595"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZM09471.1"
                     /translation="MSSHSHMPGQPQAGPQPLPGVNSIIAVGSGKGGVGKTTLAVNLA
                     IALARLGHKVGLLDADVYGPNVPLMLGTTMAPSVVGEDRILPPERYGLKVMSVGFLSP
                     GDKPLVWRGPMLHSIVKQFLGQVEWGQLDYLIVDLPPGTGDVVISLVQTVPLTGAIVV
                     TTPSDVALQDARKALEMFKQVKVDILGIVENMSYFVCPHCQHEIDIFSKGGGAR"
     gene            complement(710..991)
                     /locus_tag="VFC15_04600"
     CDS             complement(710..991)
                     /locus_tag="VFC15_04600"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HZM09472.1"
                     /translation="MQRFLTVLAIRLPQKIEERLERLAKRTGRTKAYCAKQAIIEHLE
                     DLEDVEIADQRLDKLRTGKTRTVPLSRLVRKYGVDSGQPGTLSSRQVHR"
     gene            complement(1017..1550)
                     /locus_tag="VFC15_04605"
     CDS             complement(1017..1550)
                     /locus_tag="VFC15_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09473.1"
                     /translation="MATRFTRATLFLWLAVFAASGFAQPKPASPPPAAEPLAPVAWLV
                     GGTWVTDIKDPSNGTVTHVENRIRWAPNHTAIEFNTDFNGKPHYNGFYAYNPVAKTIS
                     FYYTNSEGDMTIGTATPDPDGKTIHQDFDLLHTDGKTGHIRSTLVRDGNDAYWFGVFM
                     QKDGEWAQEFKIRYERK"
     gene            1672..>2016
                     /locus_tag="VFC15_04610"
     CDS             1672..>2016
                     /locus_tag="VFC15_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZM09474.1"
                     /translation="MAQNYLPFDFEPTEQQRRAIEHVHGPMLVVAGAGTGKTTVLARR
                     IAYLIESEAARPKEILAVTYTRHAAAQLIARVGEILYPHLDRANAARKLMSSGLRPHT
                     FHSYCYGLLRDAN"
BASE COUNT          391 a          594 c          612 g          419 t
ORIGIN      
        1 gcgcgcgccg ccgcccttgg agaagatgtc gatttcatgc tggcaatgcg ggcacacgaa
       61 gtagctcatg ttctcgacga tgccgagaat gtccaccttc acctgcttga acatctcgag
      121 ggctttgcgc gcgtcctgca atgcaacgtc gctcggggtg gtgaccacga tcgcgcccgt
      181 cagaggcacg gtctgcacca gcgagatcac gacatcgccc gtgcccggcg gcaggtcgac
      241 gatgaggtaa tcgagctgcc cccactccac ctggccgaga aactgcttga cgatggagtg
      301 cagcatggga ccgcgccaaa ccagaggctt gtcgcccggg ctgaggaagc cgaccgacat
      361 caccttcagg ccgtaacgct cgggcggcag aatgcgatct tcgccgacca cgctcggcgc
      421 catcgtggtg cccagcatca gcggaacgtt aggcccgtac acgtcggcat cgagcagccc
      481 gaccttgtgg cccaggcgcg ccagtgcgat ggccaggttc accgccaaag tggttttgcc
      541 gacgccgccc ttgccggagc ccacggcaat gatgctgttg actcccggaa gcggctgcgg
      601 cccagcctgc ggttggccgg gcatgtgcga atgagaactc acgtgtgtcc ctatagtgcc
      661 tggaatgaag tgcgcaaacg acgattatac gctgcaggaa gggaacagct tagcgatgca
      721 cttggcggct tgatagtgtc ccgggctgtc cgctgtcgac tccgtacttc cgaacgagcc
      781 gcgacaatgg caccgtccgt gtctttccag tccggagttt atcgagtcgt tggtcggcaa
      841 tctccacgtc ctccaggtct tcaaggtgtt cgatgatcgc ctgcttggcg cagtaagctt
      901 tagtgcgtcc cgtacgcttg gcaagacgct ccagccgctc ttcaattttt tggggcagac
      961 gaatagcaag cacggtcaga aacctctgca cgcaagtata gcagcgctag attggctcac
     1021 ttgcgctcgt agcgaatctt gaactcctgt gcccactctc cgtccttctg cataaagaca
     1081 ccaaaccagt aggcgtcgtt tccgtcgcgc acgagagtgg agcggatgtg gccggtcttg
     1141 ccatcagtat gcaggaggtc gaaatcctgg tggatggtct tgccatcagg atcgggagtc
     1201 gcggtgccaa tggtcatgtc accttcagaa ttcgtgtagt aaaaagagat ggtcttggcg
     1261 acgggattat aggcgtagaa accgttgtag tgcggcttgc cgttgaagtc ggtattgaac
     1321 tcgatggcgg tgtggttcgg cgcccagcgg atgcggttct cgacgtgggt cacggttccg
     1381 ttggagggat ctttgatatc cgttacccaa gttcctccga ccaaccatgc gaccggcgca
     1441 aggggttcgg cagcaggtgg cggggaagcg ggcttcggtt gtgcgaatcc gcttgcggcg
     1501 aaaacggcaa gccagaggaa cagcgtggcg cgggtgaatc gggttgccat gttcgttctt
     1561 gctccaaggt gctcgcgggt gggctgacaa ttctgctaaa tgttcctctt gccatccgcc
     1621 cgccgaccga gcataatagc atgttcgctt tttcttcgca tactgaaaag catggcgcag
     1681 aactacctac cattcgactt cgaacccacg gaacagcaac gccgggccat cgagcatgtg
     1741 catggcccca tgctggtggt ggcgggcgcc ggcaccggca aaaccaccgt cctggcgcga
     1801 cgtatcgcct acttgattga gagtgaagcg gcccgaccga aggaaattct cgccgtcacc
     1861 tatacgcgcc atgccgccgc ccagttgatc gcacgggttg gcgagattct ctatcctcat
     1921 ctcgaccggg cgaacgctgc ccggaagctg atgtcgagtg gactgcggcc tcacaccttt
     1981 cactcctatt gctacggatt gttgcgcgac gccaat
//
LOCUS       DASRKK010000212         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_601543, whole genome shotgun sequence.
ACCESSION   DASRKK010000212 DASRKK010000000
VERSION     DASRKK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601543"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFC15_04615"
     CDS             <1..313
                     /locus_tag="VFC15_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM09475.1"
                     /translation="AYATAQRTREIGIRMALGAQRSSVMRLVLADVLWLAGISIAVSL
                     PVSLLLARLLRSQLYGVSPSDPLTLLMGTLLVCAVASLAALIPARRAAFVDPMKALRS
                     E"
     gene            complement(708..854)
                     /locus_tag="VFC15_04620"
     CDS             complement(708..854)
                     /locus_tag="VFC15_04620"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZM09476.1"
                     /translation="MLWTIFAILLILWLLGWGFHIAGSLIHLLLVIAVIVLIINLVTG
                     RRAV"
     gene            complement(1316..>2077)
                     /locus_tag="VFC15_04625"
     CDS             complement(1316..>2077)
                     /locus_tag="VFC15_04625"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HZM09477.1"
                     /translation="KLGSAALLCLLAMLASAPASAVTHNVTHSAKIHKSRHSALNPIP
                     HWRFSSYAPRMYPGSHDMLVNENAELNRLQLPRIADEAELIRYEVSQVLVPVNETEAL
                     KLAADLPESRRYCRPWTRDFLQDFTQAYYEHFHTPLQVNSLVRTMEQQGQLRRHNRYA
                     APEWGDTASTHLTGVTFDLSRRGLTVEQYAWILVYMLPLKESGMIDPIEESQPVLHVV
                     VFEKYSDHLDEKNNWSEANEPTEASLSAGVGASQP"
BASE COUNT          439 a          592 c          625 g          421 t
ORIGIN      
        1 ggcgtacgcg accgcgcaac gcacccgcga aatcggtatt cgcatggcgt tgggtgccca
       61 gcgcagcagc gtcatgcggc tggtgctggc ggatgtgttg tggcttgcgg gaatcagcat
      121 cgccgtgagt ctgccggtct cgctcctgct ggcgcggctt ttgcgcagcc agttgtacgg
      181 agtcagcccc agcgatccgc ttaccctgtt gatgggaact ttgctggttt gcgcggtggc
      241 ttctctggca gcgctcatcc ccgctcgccg agcggccttc gtggatccaa tgaaggcact
      301 gagaagcgag tgatgggaga agcaattagc aattagcaat tagcaattag cagttggcgg
      361 ttgacaacct tgaatcgtag cccgcgcaga cgacggcatg gacttggccc aggacgtaaa
      421 gacgttcgta ttaagcccaa gtggggggtg ccattggagt gggagagcag cgctttcgcc
      481 ctgacgctcg cgcaaggaag tggaagatcg acctcagtcg cgtaggggcg gccggagcac
      541 gaaactaaat cgagcctcgc agaggcgaaa ctgatcagcc caccgcggaa gcggtgggaa
      601 agctggcaaa ttttctcccg agacccttca ggggcggcac tccgtgtggt gcagcgcaga
      661 atgcggggcc gcgccccacc gggtacgaca gaaacccttc gcccgttcta cactgccctt
      721 cgccctgtca ccaggttgat gatcagcacg atcacggcta tcaccagcaa aaggtggatc
      781 aagcttccgg cgatgtgaaa gccccatccc aacaaccaga gaatcagcag aatcgcgaaa
      841 attgtccaaa gcattcttgt cacctacggt cagcacggct gccgtaagca ataggaatca
      901 aaattccgtg ctggcgttgt atgggcgatc aaattcccgg gtgacggcag gttctgccca
      961 gacgcaaacg gcaccaatct tcttcgggcc gcgccgcatc gtccaactca tctcataaat
     1021 cgttctgggt cgtatcaggg cacggctttt agtcgtgccg aacgaagcct agaaaaggtc
     1081 agggctttta gccgctgcaa aaataggctc ctcaggggct gaagcccaag tcgttgttga
     1141 ccggttgcgg cacgtctgaa gccgtgccct tatacaaaac ggcttatgaa atggctcaag
     1201 aacatccgat ccttcgactt gcctgtcgcg gaccggcgaa cagcggatag caaagagcca
     1261 gatttagctg aaagctgacg gctgatagct gacgactgac ggagtaggaa ttctgctaag
     1321 gctggctcgc gcctacacct gccgacaggc tcgcctcggt aggctcgttg gcttcggacc
     1381 agttgttctt ctcgtccaaa tggtcggagt atttctcgaa gaccaccaca tgcaggaccg
     1441 gctggctttc ctcgatcggg tcaatcatgc ccgactcttt caggggcagc atgtagacca
     1501 ggatccacgc gtactgttcg acggtcaagc cgcggcgcga caggtcgaag gtaactccgg
     1561 tcaagtgggt ggaagccgtg tctccccatt ccggcgccgc gtaacggttg tgacgccgca
     1621 gctgtccctg ttgctccatg gtgcgcacca gcgaattgac ctgcaatgga gtgtggaagt
     1681 gttcgtagta ggcctgggtg aaatcctgca ggaagtcacg cgtccacggc cggcagtagc
     1741 gccggctctc tggcaggtcg gctgccagct tcagcgcctc agtctcgttc actgggacca
     1801 gcacctgcga cacctcgtac cgtatgagct ctgcctcatc ggcgattcgt ggcagttgca
     1861 ggcggttcag ctctgcgttc tcattcacga gcatgtcatg cgagccggga tacattcggg
     1921 gtgcatagct gctgaaacgc caatgcggaa tgggattgag agcagaatga cgactcttat
     1981 gtatcttggc gctatgtgtc acattatgcg tcaccgcgga cgccggagcg ctggccaaca
     2041 tggcgagcag acacagcaac gccgcagaac ccagctt
//
LOCUS       DASRKK010000213         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_308585, whole genome shotgun sequence.
ACCESSION   DASRKK010000213 DASRKK010000000
VERSION     DASRKK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308585"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..335
                     /locus_tag="VFC15_04630"
     CDS             138..335
                     /locus_tag="VFC15_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09478.1"
                     /translation="MPSTLRRRRFPTQDIEIAVVRAYFVKSILGAVPLVKYLLDHVLA
                     ILKPKSNRPFVRRPAGVAIHL"
     gene            742..1197
                     /locus_tag="VFC15_04635"
     CDS             742..1197
                     /locus_tag="VFC15_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZM09479.1"
                     /translation="MRSNEIATIGQSLVVKGDLSGSEDLVVDGEVDGSIALHGQSLTV
                     LPNGRVRANIEAGDVIVHGRVDGDIHASDRVELHKSASLTGNISTARILIEDGAFFKG
                     TIDMQKPQPAPKIETKPPATATAAVPVAKEQGSLLERLAARKSRGSWSK"
     gene            complement(1795..2589)
                     /locus_tag="VFC15_04640"
     CDS             complement(1795..2589)
                     /locus_tag="VFC15_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09480.1"
                     /translation="MTAAFIHPMASFIEKTFVITEFELRKLRHDFTELITRALQPALW
                     LLIFGQVFSRSGAMHTGNIPYLDFIAPGILAQSVLFVAIFFGINVIWERDLGIVQKFL
                     ASPTPRAALVLGKGFSAGFRTLSQAAIVYGLSLLLGVKLNWSPFALVNVLIIVVLAAT
                     LFSTFSLIIACIVKTRERFMGVGQVLTMPLFFASNAIYPTDIMPGWLKVVSHLNPLTY
                     VVDALRTFMLAGSASTFGLGRDYAVILLTMTILVFIGARLYPRLAS"
     gene            complement(2600..>2814)
                     /locus_tag="VFC15_04645"
     CDS             complement(2600..>2814)
                     /locus_tag="VFC15_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09481.1"
                     /translation="LEEAESHCDRIAIMHLGKVAALGTCKELEASLGAGHTLNDVFVH
                     YAGSALDSGGTFRDVSAERATAQRLG"
BASE COUNT          627 a          799 c          869 g          519 t
ORIGIN      
        1 ggttcatcgc catcggttat gctgcaactt tctcaagtcg gtactcatgg tgtaatccac
       61 ccagcacttc caagtcccgc gcatgagagc gttttgttag ctcagttctc tcgtggcagt
      121 tgccggaatg acaccaaatg ccaagtactc tccggcgtcg acgtttcccg acgcaggata
      181 tagagattgc cgtcgtccgc gcgtactttg taaaaagcat cctgggggcc gtaccactgg
      241 tcaagtacct cctcgaccat gtactggcaa tcctcaagcc gaaatcgaac cggcctttcg
      301 tccgccgtcc ggccggagta gcaatccacc tctagcttca tctcccccat tgtgacgcat
      361 cggcgctaca cccgccagcg ccgctgcacc agcgtggccc gggactcgga gactcccatg
      421 cagcctccgc ggtcaaggat gccgcgagga aggactggtg cgcgagttgg acgaacgtct
      481 cggattgagt tcgctccaca tcacgggcgg ctgacggggc actgaaagct gagatggagt
      541 attgctgccg gcggcagtca ggtgtatgtt actggccact cagaaataaa aaaggaaatg
      601 tcggtcaaca tcatgtggaa gtcatgggga acggaagacg aactctcgac accagctaac
      661 gagccgagca cacccggctc tccatcgatt ctggccgtga gtgcgccatc gggcgcggcc
      721 gaagctgtgc gcagccacag cgtgcgcagc aacgagatcg ccaccattgg acagtcgctg
      781 gtggtgaaag gcgacctttc gggcagtgaa gatttggttg tagacggcga agtagatggc
      841 tcgatcgcgc tgcacggcca gagcctgacg gttcttccca atggccgcgt gcgcgccaat
      901 atcgaggctg gcgatgtgat tgtgcacggc cgggtggacg gtgacatcca cgccagcgat
      961 cgggtcgagt tgcataaaag cgcatcattg acgggcaaca tctcgactgc gcggattttg
     1021 atagaagacg gtgccttctt caagggcacg attgacatgc aaaaaccgca acctgctccg
     1081 aagatcgaga caaaaccgcc ggcgaccgcg actgcagccg ttccggtggc caaggaacag
     1141 ggatcgcttc tcgaacgact ggctgctagg aaatcgcgag gaagctggtc taagtagctg
     1201 gtagtcggta gtggacgtga ccaattcgcg cgaagaaggg gtagcgcgcc ggctgtcagc
     1261 cgtgaccgcg gaagtcttga ccgcggcgcg gcatagggta tcgccctcaa tccagtgttc
     1321 gtacaggccc aggcagtagt actggcgccc cgggctttcg tccgtccgct cgccggcgta
     1381 ctccttccct tatcagagcg gcggcgactg gcgaagctcc gggcgctcga cctgccgatc
     1441 tggaattacg cggcgccacc ggcgacggtg acctagtcca aatttatcgt cggtggctaa
     1501 ccgtacgaaa aagccggagt ctgcaccaag agtcatgcag attgcgactt gccgaggttt
     1561 ttgcggagga caggtagaga aggcaatccg aagaaactag tcgaggctgc aagccgcgcc
     1621 tttccacccc gttcgattat tttgaccata cggggtggca ggagatgatc ttcttcgaca
     1681 gattcagttc atcgacaacc ttttcgactt ggcagcctag gaacgcacga cagacgctcg
     1741 gcgatgaact tgtctgcatt tgaaacttgc aacactaccg tttccagtga cgacttacga
     1801 tgcaagtcgg ggatacaacc gcgcgccaat gaaaaccaat atcgtcatgg tcaggaggat
     1861 gacagcatag tcgcgtccca gaccgaaggt gctcgcgctg ccggcaagca tgaaagtgcg
     1921 cagagcatcg accacgtagg tcaagggatt tagatgcgaa acaaccttca gccaaccggg
     1981 catgatgtcg gtggggtaaa tcgcgttgct ggcaaagaac aacggcatgg tgagcacctg
     2041 gccgacgccc atgaagcgtt cgcgcgtctt cacgatgcac gcgatgatga gcgaaaacgt
     2101 cgagaacagc gtcgcggcca ggaccacgat gatcagcacg ttgacgagcg cgaacggact
     2161 ccaattcagc ttcaccccga gcagcagcga gaggccgtaa acgatggccg cctgcgagag
     2221 cgtacggaaa ccggcggaga agcccttgcc aagcaccaat gccgcgcgcg gagtcgggct
     2281 ggcgaggaat ttctgcacaa tgccgaggtc acgctcccag atgacgttga tgccgaagaa
     2341 gatggccacg aagagaacgc tttgcgccag gatgccggga gcaatgaagt ccaggtaagg
     2401 aatattgccc gtgtgcatcg ccccgctgcg ggagaacact tgcccgaaga tgagcagcca
     2461 aagcgcgggc tgcagtgcgc gggtgatgag ttccgtgaaa tcgtggcgca gcttgcgcaa
     2521 ctcaaactcg gtgatgacga aggtcttctc tatgaagctg gccatggggt ggatgaaagc
     2581 ggcggtcata gggttggggc tagccgaggc gctgggccgt ggcgcgttcg gcggacacgt
     2641 cgcggaaggt gccgccggaa tccagggcgc tcccggcata gtggacgaag acgtcattca
     2701 acgtgtggcc ggcgccaagc gaggcctcga gctctttgca ggtgcccagc gcagccacct
     2761 tgcccaggtg catgatcgcg atgcgatcac aatggctctc ggcctcctcc aggt
//
LOCUS       DASRKK010000214         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_406724, whole genome shotgun sequence.
ACCESSION   DASRKK010000214 DASRKK010000000
VERSION     DASRKK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406724"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..401)
                     /locus_tag="VFC15_04650"
     CDS             complement(261..401)
                     /locus_tag="VFC15_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09482.1"
                     /translation="MAAGWPYFLQLHLLQWASVALLLAQTGARRAAWRSGRLLDPEMQ
                     AP"
     gene            476..1645
                     /locus_tag="VFC15_04655"
     CDS             476..1645
                     /locus_tag="VFC15_04655"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM09483.1"
                     /translation="MRIVALDLGVRKTTYCEISQGKVVQRATVSEVASLQSLLGPEQA
                     AATVAIEACREAWHVHDLLVEWGNEVVLVDTTRSRQLGIGQHGRKTDRIDAETLARAL
                     EVGRIPAAHVLSPKRRELRRVLAVRRALVESRAQFVTTLRGLVREQGGKIPSCDTAQF
                     VRKVRAQKLASEVFKLVEPLLILVETIETQLANTEEQLALLCLQEPLIAVLTTTPGVG
                     MVIAATFMSVIDEAKRFQSAHQVESYVGLVPCEDSSGGKRRLGAISKKGNSYLRTLLV
                     QAAWVILRNGDKSDPLYLWVNKLAERRDKRIAVVALARRLVGVLWAMWRDGTVYDAQH
                     LAQQGTRGLRGAVQSIEQQSKALENAAKKHSVKRHTKRSIATANRGQKTPAVNAA"
     gene            2083..>2300
                     /locus_tag="VFC15_04660"
     CDS             2083..>2300
                     /locus_tag="VFC15_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09484.1"
                     /translation="MVIVRADAFALAEGNNRAPRGGETVTMMWIEAPTGRCGVEVKTV
                     GNSNSPHLRSSSKVRRNGLTGVCEQGMST"
BASE COUNT          575 a          634 c          699 g          392 t
ORIGIN      
        1 acagcaacag gagagttcta tgccaaaaac cactgtctcg cgaaccgcca ccaaaccgcg
       61 gaacacaacc agcgaaaaaa gaacgagtaa actgttgacg tctggcccga ctcatagtcg
      121 agtcttgggc ggcccgacga gcgcgaaagc gcgaaggacg gcagtcaaga gatgggatga
      181 gtcgggcgag aggaaccgaa gcgtcatgtc ggcaatcaac aagagcaaat tgagtcccaa
      241 aagtcaggac gcggcgcccg tcacggcgct tgcatctcgg gatcgagtag acgtcccgac
      301 cgccaagcgg cgcgccgggc gccggtctgg gccagcagca gtgccaccga cgcccattgg
      361 agcagatgaa gctgcagaaa atagggccat cctgccgcca cccggcgaaa atcccgacgg
      421 cgcccctacg cgttctgaga cgcaaaatgt ccaaggggcc aaaggcagga tgaccatgcg
      481 tatcgtagca ctcgacctag gcgtaagaaa gacaacgtat tgtgagatct cccaagggaa
      541 agtcgttcaa cgcgcgactg tgagcgaggt agcctcgttg caatcgctgc tgggaccgga
      601 acaggcggca gcgacggttg caatcgaggc ttgccgcgag gcttggcacg tgcacgacct
      661 tctggtcgag tggggcaacg aggttgtgct ggtagacacc acgcgcagcc gacagctggg
      721 gatcggtcaa catggtcgca agaccgatcg cattgacgcg gagaccctgg cgcgagcact
      781 tgaagtcggc agaattccag ccgcccatgt gctgtcgccg aagcgacgtg agctgcgccg
      841 tgtcttggcg gtccgcaggg cactggtcga atcgcgagca cagttcgtca ccactctgcg
      901 agggttggtg cgcgagcaag gaggtaaaat tccgagttgc gacacggcgc aatttgttcg
      961 taaggttcga gcccagaagc tggcgtcgga ggttttcaaa ctcgttgagc cactgcttat
     1021 attggtggaa accatcgaga cgcagctagc aaacaccgag gagcaattgg cactcctttg
     1081 tttgcaggaa ccgctcatcg ctgtactgac aacgacgcct ggcgtgggga tggtaattgc
     1141 cgccactttc atgtcggtga tcgacgaagc gaagcgattt caaagtgctc accaagttga
     1201 atcatacgtg gggctagttc cctgcgagga ttcgagcgga ggcaaacgtc gtttgggggc
     1261 catctccaaa aaggggaata gctatttgcg tactctgcta gtacaggccg catgggtaat
     1321 cttgcgcaac ggcgacaaga gcgacccgct ttacctttgg gtcaacaagc tagcggaacg
     1381 gcgagacaaa cggattgctg tcgtcgccct ggcgcgacgt ttggtcggcg tactatgggc
     1441 gatgtggcgc gatggcacgg tgtacgacgc gcaacatcta gctcagcaag gtactcgcgg
     1501 cctacgtggt gctgtgcaga gtatcgaaca gcagagcaaa gcgcttgaga atgcagccaa
     1561 gaaacattca gtcaaacgcc ataccaaacg ttcaatcgca acggccaatc gaggtcaaaa
     1621 aactccggcg gtaaacgccg cctgattcgt gtgaactgcg ccaagagcgt gatattcaga
     1681 gcagcctaga tcggtcggtg gatgagcgcg gtgtctacgc tggcggacga cccaggtcgt
     1741 cagagcccgt cgaagagaat gcgccgccac caaaccgctt cggaccccca actgcacggg
     1801 aatcaaaatg accgaacgag gtgagattcc ctaagtgcga atagtcgatc gagagcagct
     1861 cgtcgttttc ccgctcgcca gcgcaatagc aataacccaa ttccgaaaag cggctgcagc
     1921 gcagccacag gagacttgca tgtcaaaaac cacggtatcc aagactgtta ccccgcgaag
     1981 gacaacagcc cgtgaaaaaa ccgcaactaa acagttgacg tcgtgcccga ctcatagtcg
     2041 tagacagcga gtgaacaggc cgtgtgattg agccccgata tcttggtcat cgtgagagcc
     2101 gacgcatttg cgttagcgga aggcaacaac cgagcgccac gaggcggcga gaccgtgacc
     2161 atgatgtgga ttgaggcacc gacaggacgc tgcggcgtcg aagtgaaaac cgtcggtaac
     2221 agcaactccc cgcatcttcg gtcatcctca aaggttaggc gcaacggact caccggggtc
     2281 tgtgagcagg gcatgagcac
//
LOCUS       DASRKK010000215         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_406741, whole genome shotgun sequence.
ACCESSION   DASRKK010000215 DASRKK010000000
VERSION     DASRKK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406741"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1000..1998
                     /locus_tag="VFC15_04665"
     CDS             1000..1998
                     /locus_tag="VFC15_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM09485.1"
                     /translation="MRFNQLGRREFITVLGGAAAWSLSARAQETRGAKIPEVGFFYPG
                     TVEASKARITSFLKGLQEKGYVEGQNVLLVTRLTELHAERLAPLAAELVRRKVDVILS
                     VGSPAVKAARAATTVIPIVALDLESDPVNSGFVASLSHPGGNITGLFFDFPDFSAKWL
                     ELLMEVLPGLARVTILWDPATDPVQLNGVNAAAASLGLTLQVLKVETLAEMEDAFQAA
                     VQARTQAVMLLSSPFFGANLKPAANLAARYQLPAITLFPEFAEVGGFMAYGTNLMYLY
                     QQTGALVGKVLSGARPADTPVERPTRFHLVINLKTAKALGLTISHALLTRADEVIE"
BASE COUNT          524 a          694 c          744 g          492 t
ORIGIN      
        1 gaccagtgca gcgaggctag ctgccattcc tgttgtagtc atatccagca gcgggaaggg
       61 cgaccgaccc gctgaaagcc tggaagtaaa agcccccatt ggccgggaga ccggtcaggt
      121 ccgtactggg gggccagcaa cctgacaggc cgtaacagtt gcgaaccaga gtagccccga
      181 aaaccgtgcc ccgcgcgaaa cggatttgcg ggaacatcgg gagtgccgag ccctttggtc
      241 catggcgaag gccaatgtcg acgtcggtat tctggacaga aacgacgatc gaactccccg
      301 gggtcagaga gggcggcatg tcaggacgga atgggcagcg aaagcccgga accactcggg
      361 ggtcgcttag gcgctcccgc acagcgaagg catcgcgtat aaaccgccat gcggtgaaat
      421 cgcgatgtgc ccgcgagtgg gacggatggg gccgaataag tgacgacgga ccgggacact
      481 ataacccgga cttgagcgag ggcccctggg gcggaggagt aatgaccctc cacggcggtg
      541 cgcaatcgag tcggcggccc gacacagagc gggacaaccg ttgagaccac gaggcgcacg
      601 aagggcggat gcaaactgga cgtcggacgg cgcatgccgg gagcaggctt aagccgctga
      661 tcacccagga aggcaccgct tggaaagcca gccttccagc cgtactgggg aaaacccgcc
      721 gtacggaatg atagggggga tcgaggaaac gtcggcatca ttcgaagccc ggtccgcgcc
      781 tcgatcccac ccgaccataa gcgacattga gtgtgcgtcg cagcacccgt tttggacatc
      841 cgatctgcga cgccttcgct catatgattg acttagatca atttttcaca ttcggaatgg
      901 cgtgacactt catcgtttac ggatccgatc ctccaccttg atcggggcaa gaactatttt
      961 gcgcagaatg cggtgcgacc accgaaccgg taggggcaca tgcgcttcaa tcagctagga
     1021 cggcgcgagt tcatcaccgt tctcggcggt gctgcggcat ggtcgctctc cgcgcgggcg
     1081 caggagacac gtggcgcgaa aatcccggag gtcggctttt tctaccccgg caccgtggaa
     1141 gcatccaagg cgcggatcac ctcgttcctc aaagggttgc aggaaaaagg ctacgtagag
     1201 ggtcagaatg tcctgcttgt cacgcggttg acggagctgc acgcggaacg actcgcgcca
     1261 ctcgcggccg aacttgtccg tcgcaaagtt gatgtcattc tgagtgttgg ttctccggcg
     1321 gttaaggccg cacgcgccgc aactacggtc attcctatcg tcgctctgga tctggaatcg
     1381 gatcccgtga acagcgggtt cgtcgcaagc ctgtcgcacc cgggcggaaa catcacggga
     1441 ttgttcttcg attttccgga tttcagcgca aagtggcttg aactgttgat ggaggtgttg
     1501 ccgggattgg ctcgcgtgac aatcctgtgg gatccggcaa ctgatcctgt gcaactgaat
     1561 ggggtgaacg ccgccgccgc atcccttggc cttacgttgc aggttttgaa ggtggaaacg
     1621 ctcgctgaaa tggaggatgc cttccaggct gccgtccaag ctcgaacaca agcggtgatg
     1681 ttgctgtcat cgcctttctt cggggcgaac ttgaagccag cggcaaacct cgccgcgcga
     1741 taccaacttc cggcgatcac attatttccc gagttcgctg aagtaggtgg ttttatggcc
     1801 tacgggacga acttgatgta tctgtatcaa caaacaggcg cgcttgtcgg caaggtactg
     1861 tccggtgccc ggccagcgga cacgccggtc gaacgaccga cgcggtttca tttggtcatc
     1921 aacctcaaaa ccgccaaggc gcttggcctg acaatttcgc atgcgctact cacccgcgcc
     1981 gacgaggtga ttgaatagat atgctgtttg ctgcggtgca tgagtccgct tttggcactt
     2041 cgcggacaaa ccgatggcac cgggttttgt cgcctattgg actaacaacg gacaaaggtg
     2101 gatttcggcg cgagttggct atgacgtgaa tgacccaggc tgtgtgaaaa cttcaacgtc
     2161 cgccgcgcgc gtagaaatga ttttgagaaa ttgcgcaata tgagaactga taatgctgcg
     2221 gacatacgat tgaatgtcat gttggtgaat tgtattttct acatttctcc gatgtatgag
     2281 ttttcacaca gtctggaccc gaggcggacg tctcccggcc ctcaacgacc tgccgcctga
     2341 gcccacgccg gccccttccc ccgagctagt ttccgccggt acgatgcgtt ttcctagcct
     2401 tcgctcatac ccgaccagcg gacgacgcaa gactattgac tgcctcgggc tagt
//
LOCUS       DASRKK010000216         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_895178, whole genome shotgun sequence.
ACCESSION   DASRKK010000216 DASRKK010000000
VERSION     DASRKK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895178"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFC15_04670"
     CDS             complement(<1..240)
                     /locus_tag="VFC15_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09486.1"
                     /translation="MRKRFYILFVARDAEGQLRKIPIPIHYLYVFMAGALIGMFTITG
                     MAGSYTRMLWKVASFNQLRSEKNVLQTRYKQLEQVN"
     gene            574..1515
                     /gene="thiL"
                     /locus_tag="VFC15_04675"
     CDS             574..1515
                     /gene="thiL"
                     /locus_tag="VFC15_04675"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZM09487.1"
                     /translation="MPLKEKLLIERVRRAATVRGQRAGHGIGDDCAVLPLPRGHEALV
                     TTDFSLEDIHFRREWHPPDSVGHRCLARGLSDIAAMGGIPRAAFLSLALPADLPQSWV
                     DKFVAGLLKLATRFSVSLAGGDTAQSPDGVLADIVVLGSVPAGKAILRSGARPGDFLY
                     VTGTLGAAVAALNQLRDGESLRPRSHLKHFYPEPRLAVGQFLRAKKLASAMIDISDGL
                     STDLGHICEESGAGAVVYAEALPAAAGKDGLALALHGGDEYELLFTARPQRRIPKQIA
                     GVPVTRIGEILSGKQMRLATADGKTQVLKPAGWEHFG"
     gene            1635..3185
                     /locus_tag="VFC15_04680"
     CDS             1635..3185
                     /locus_tag="VFC15_04680"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZM09488.1"
                     /translation="MNLEEKLIELKRRDSLAEAGGGEERRAREHKQGKMSARERIEFL
                     LDEGTFEETDKLVTHRCTDFGMDEQKYFGDGFLTGYGRIEGRLVFVFAQDFTVFGGSL
                     SETNAAKIVKIMDMAMRVGAPVIGLNDSGGARIQEGVMSLAGYADIFLRNVLASGVVP
                     QISAIMGPCAGGAVYSPAMTDFILMVKNTSYMFITGPEVIKAVTHEEVTKDELGGAMT
                     HNEKSGVAHFMAHDDAECLSMIREMLSFIPSNNLEDPPRKACSDPIDRADERLDTIVP
                     TESNQPYDIKDVINAVVDDSYFFEVQEHYAKNIVIGFARLNGKPVGIVANQPAFLAGV
                     LDIKASIKGARFVRFCDCFNIPLITFEDVPGFLPGTQQEYGGIITHGAKLLYAFSEAT
                     VPKITVITRKAYGGAYCVMASKHIRTDVNYAWPTAEIAVMGPEGAVNIVYRHELEKSE
                     NPEADRKAKIEEFRERFANPYIAAERGYLDAVIRPRDTRRKLIQALDMLENKRAKNPP
                     KKHGNIPL"
BASE COUNT          824 a         1201 c         1175 g          742 t
ORIGIN      
        1 gttgacctgc tccagttgtt tgtagcgggt ctgcagcacg ttcttctcgg agcgcaactg
       61 gttgaagctg gccaccttcc acagcatgcg ggtgtaagaa cccgccatgc cagtgatggt
      121 gaacatgccg atcaaagcgc cggccatgaa gacgtagagg taatgaatag ggatgggaat
      181 ctttcgtagc tggccttctg catcgcgagc tacgaaaaga atataaaagc gttttcgcaa
      241 aactatgacc ctcagttaaa aatggttccg gaactgtgaa acaacaaatt atgaaaatcc
      301 ggaacttcag gccttgccgg tccgaactgt caacttactt acgggtacct ggacaaccgt
      361 gggttaacgg ttttcctccc aaccgtcagc cgattggcca actgactggg tgcatggctg
      421 gcgtaatgaa tgacatcaaa ccgtaacaga gccttaataa cctgtcaacg aattatggga
      481 taagtgaccg atactaaagt gtgatatgcc cggcagccgc attacccgtg gcctcgggta
      541 tatttgggct ggctgcttta ccacggaggc gcagtgcccc tgaaagaaaa gttgctgatc
      601 gaacgagttc gtcgcgcggc aaccgtgcgc ggacaacgcg ccggccacgg catcggggac
      661 gactgcgcgg tgcttccgct tcctcgcggg cacgaagcgc tggtcaccac ggacttcagc
      721 ctggaggaca tacacttccg gcgcgagtgg catccgcccg attccgtggg tcatcgctgc
      781 ctggcacgcg ggctcagcga cattgcggcc atgggaggga tcccgcgcgc ggcgttcctc
      841 tcgctggcgc ttccggcgga tctgccccag agttgggtgg acaagttcgt tgcagggcta
      901 ttaaagctcg caactcggtt ttcagtttcc ctcgctggag gcgacacggc gcagtcgcct
      961 gacggtgtac tcgccgacat cgtggtgctc ggcagcgttc ccgccggcaa agccatcttg
     1021 cgctcgggcg cgcgacccgg cgatttcctc tacgtcaccg gcacgctcgg cgcagcagtc
     1081 gctgctctca accaactccg cgacggagaa agtctgcggc cgaggtctca cctcaagcac
     1141 ttctatcccg agccccggct cgccgtcggc cagttcctgc gcgcgaagaa gctggcctcc
     1201 gccatgatcg acatcagcga cggactctcc accgacctcg gccacatctg cgaagagagt
     1261 ggcgctggcg ctgtggtcta cgccgaagct ctgcccgccg ctgctggcaa ggatggcctg
     1321 gcgctggcgc tgcatggcgg cgacgaatat gagttgctct tcacggcccg tccccagcgc
     1381 cgcattccga agcagatcgc cggcgttccc gtcacccgca ttggcgagat cctgagcggg
     1441 aagcagatga gactggccac cgccgatggc aaaacgcagg ttctgaagcc cgccggatgg
     1501 gaacacttcg ggtgagcggg tgcgtcatga caatcacgca aacctccagc aagtaccgcc
     1561 gtcactttgc tcggtgagcc tgtaatcgca aactctcgcg gctcctcggc ccattgctcg
     1621 ttataatcac gctcatgaac ctggaagaga agctgatcga actgaagcgc cgtgactcgc
     1681 tcgccgaagc cggtggaggc gaagaacgcc gggcgcgcga gcacaaacag ggcaagatgt
     1741 cggcgcgcga gcgcatcgag tttctgctgg acgaaggcac cttcgaggag accgacaaac
     1801 tggtcaccca ccgctgcacc gacttcggca tggacgagca gaagtatttc ggcgacggct
     1861 tcctcacggg ttacggccgt atcgaaggcc ggctggtgtt tgtcttcgcg caagacttca
     1921 ccgtcttcgg cggctcgctg tcggagacca acgcggccaa gatcgtcaag atcatggaca
     1981 tggccatgcg cgtgggtgcg ccggtcatcg gcctgaacga ttccggtggc gcgcgcattc
     2041 aggaaggcgt gatgtcgctg gcgggatacg ccgacatctt cctgcgcaac gtgctggctt
     2101 ccggcgtggt gccgcagatc tcggccatca tggggccgtg cgccggcggc gccgtctact
     2161 ctccggcgat gaccgatttc atcctgatgg tgaagaacac ctcgtacatg ttcatcaccg
     2221 ggccggaagt cattaaggcc gtgacccacg aagaagtcac caaggacgaa ctcggcggcg
     2281 cgatgacgca caacgagaag tccggcgtgg cgcacttcat ggcgcacgac gatgccgagt
     2341 gtctcagcat gattcgcgag atgctcagct tcatcccctc gaacaatctc gaagatccgc
     2401 cgcgcaaggc ttgctccgat cccatcgacc gggccgacga gcgcctcgac accatcgttc
     2461 ctaccgaatc gaaccagccc tacgacatca aggacgtaat caacgccgtg gtggacgaca
     2521 gctacttctt cgaggtgcag gagcattacg cgaagaacat cgtgatcggc ttcgcccgcc
     2581 tgaacggcaa gccagtgggc atcgtcgcca accagcccgc gtttctggcc ggcgtgctcg
     2641 acatcaaagc gagcatcaag ggagcgcgct ttgtgcgctt ctgcgactgc ttcaacattc
     2701 cgctcatcac ctttgaagac gttccgggat ttctaccggg cacgcagcag gagtacggcg
     2761 gcatcatcac gcatggcgcc aaattgcttt atgcgttctc cgaggcgacc gtgcccaaga
     2821 ttaccgtgat cacccgcaaa gcttatggcg gagcctattg cgtcatggcg tccaagcaca
     2881 tccgcaccga cgtgaactac gcctggccaa ccgccgagat cgcggtgatg ggccccgaag
     2941 gcgcggtgaa catcgtgtac cggcatgagc tggagaagtc ggagaaccct gaagccgatc
     3001 gcaaggcgaa gatcgaggag ttccgcgagc gctttgccaa tccttatata gcggccgagc
     3061 gcggctacct cgatgcggtg attcgcccgc gcgacacgcg ccgtaagctg attcaggcgc
     3121 tcgacatgct ggagaacaag cgcgccaaga acccgcccaa gaagcacggg aacataccgc
     3181 tgtgagaggc ctagctagct agccaaaaga agctagcccg tcagggcgga acaagattag
     3241 cccaggacgt aagtcctggg tagcctggtc atatgaatcc caagccggct tcagccggcg
     3301 acaccatcgc gtgggcgtga ttaccagcgg ggctcatcgt ccgctcccat cctgtccgca
     3361 aagcagccga caaagatagg tacccccggc ccactcggca tccaacgtgt gtttgtagta
     3421 gatagagttg gaatcgccac cgcgcgagtc ccgccttgcg ggacgcaaga gcttagccca
     3481 gcgctttagc gctgggaaac gagcaccaag aactcagagt gccgtaggca cggcagagcg
     3541 ttagccccgc atgtattctc tgtcgtccct acgggactcc ggttcaatat gccgttgtcc
     3601 cagccttgaa aggctgggct aagctctttc gtcccgctgt gcgggactgg cgcgtgatgg
     3661 ctccaacgcc cgaccggcac cagcagttcg tggacagccc agctccagga tttcgagatg
     3721 ttcagggttt gccattgccg cgtgacctcg cttgcttccg ggatactgcg tatcgctcga
     3781 cgtaccagtc ccgctctgcg ggacgaaaca gcttagcagc ccgtggtgca agtctggatt
     3841 cgtaagcaaa aacatctgag gtatccacag acattgtttg tagaattgcc ctatggcgat
     3901 gggcacgcgc aagcagcggg agaagcaaga ggggttgtgg at
//
LOCUS       DASRKK010000217         7658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_15874, whole genome shotgun sequence.
ACCESSION   DASRKK010000217 DASRKK010000000
VERSION     DASRKK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15874"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..710
                     /locus_tag="VFC15_04685"
     CDS             204..710
                     /locus_tag="VFC15_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09489.1"
                     /translation="MAELFTPAQPPAQQRNWMPIVVGLIAVLVVIGIIVALTRSHGPT
                     GPQTNPYAAKLQLSNPKLSAAENYVGGTVTYLDVDIANTGDRALIGADMKVVFKNTLD
                     QVVQTETLPLHVLVENQMGGYPDLVDLGRSPIGPGQTKTVRMTLEHISADWNRSYPEI
                     QLVNLKLK"
     gene            871..3234
                     /locus_tag="VFC15_04690"
     CDS             871..3234
                     /locus_tag="VFC15_04690"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZM09490.1"
                     /translation="MALLTEADTCRRYVLPKLYAAGWSDEQINEQKTFTDGRIIVAGS
                     KVRRLAQKRSDYLLRYRRDFPIAVVEAKSIYAKPGDGLQQAKDYAQILGLKFAYATNG
                     HGIIEHDFLTGRDNDVEGFPAPDELWGRIRGSEGIDDATAERVLAPCYHLSGKSPRYY
                     QEIAINRAVQAILQGKRRVLLTLATGTGKTVIAFQVCYKLWNSRWNRTGEYRRPRILY
                     LADRNILVDDPKDKIFTPFGEARWKIEGRANKGREMYFATYQAIAKDDRRRGLFREFP
                     RDFFDLVVVDECHRGIARDESNWREILEHFEPAFQLGMTATPLRDDNRDTYAYFGKPL
                     YLYSLKQGIEDGFLAPYRVHRILSTVDAAGWRPTKGELDRYGREIPDGEYETKDFERL
                     VALKARTEAIARNLTDFLKATDRFNKTIVFCVDQEHAEEMRKALNNCNTDLVEQYPDY
                     VARVVSDEGKIGRGHLDRFMELETLVPAIVTTSQMLTTGVDVPTCKNIVLARVINSMT
                     DFKQIIGRGTRVRDDYGKLYFNILDYTGSATRLFADPEFDGPPALLTEEQMDASGRQI
                     ESKVLEEEQVENTEELEIPTIDELDKGDGELRRKYYVDEGTVKIAAHLVYELDVNGKQ
                     LRVVKFTDYTAENLRSMYPSAAALRSKWSSAQERAEIIQALEDRGISFEKLAEVANQP
                     DADPFDLLCHVAFNAPLRTRRERAEMLKREKTDFFQQYGPDAREVLTQMLEKYVEYGT
                     AQFQIPEVLYVTPFSDRRSVSEIVALFGRADQMRTAVNDMQTLLYAS"
     gene            3238..4788
                     /locus_tag="VFC15_04695"
     CDS             3238..4788
                     /locus_tag="VFC15_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZM09491.1"
                     /translation="MTRKTKTEAPRSTAQQLGSIIKSSRDTMRKDKGLSGDLDRLPML
                     TWIMFLKFLDDMEDLRAEEAKLAGKRFRPAIEPPYRWRDWAAPRDGIGMTGPELIAFI
                     NQEEAVRPDGKKGPGLFAYLRLLHSANGDRRDVIAKVFEGTVNRMINGYLLRDAVNKV
                     NEIHFASRDEIHTLGQLYESMLREMRDAAGDSGEFYTPRALVKFIVTVVNARIGETVL
                     DPAAGTGGFLVEAFEHLKKQCKKAEDFTLLQKATLHGIEPKPLPYLLCQMNLLLHGVE
                     YPEIDSLNALRFPLREIGDRDRVDVIMTNPPFGGEEERGILSNFPEDKQTAETALLFL
                     QLIMRKLRRPVGGHKGGRCGMVVPNGVLFGDGVCARIKEELLKEFNLHTIVRLPNGVF
                     APYTSIPTNLLFFERSGPTKEIWYYEHPLPEGRKNYTKTAPVQFEEFSDCVKWWKMRE
                     ENDHSWKVSAADLLAAGCNLDRKNPHAKEDITHLPPEQLAESIMQKEQRIAEIMLNIK
                     ELLTKYDS"
     gene            4827..5978
                     /locus_tag="VFC15_04700"
     CDS             4827..5978
                     /locus_tag="VFC15_04700"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZM09492.1"
                     /translation="MRRVERFESRDNLTEYPFAGTYSFARGIFVGERKQGSTFSLPKI
                     QRIREGDFVYCKIMAWEGAFGIVPREADNCVLSGAFVVYELNSEYIDQSFLDYFFKVP
                     AHWQRIGRQSSGTNVRRQSLHPVQFEKAEIPLPPLAEQRRIVTRIEQVAAQVNEARAL
                     RQQAVNEAEGLWARGAARTFEQIEQAFPLRCVADVVSVRGGGTPSKANPFYWSGSIPW
                     VTPKDMKVREIHGAIDHISSQAITESSAKLIEPGAVLVVVRGMILAHTFPSAILRVPA
                     TINQDMKALIPKPDVLPEYLCAFLWAYNDQILGRVEKSTHDTRKLETPVLMSIKLPVP
                     PLAEQRRIVAQLDDLQMKTNALMALQSETSAEIDALMPSILDKALRGEL"
     gene            complement(6450..7256)
                     /locus_tag="VFC15_04705"
     CDS             complement(6450..7256)
                     /locus_tag="VFC15_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZM09493.1"
                     /translation="MGRIIAVTNQKGGVGKTTTAINLAASFASLGVRTLLVDCDPQSN
                     ASSGLGFLRDPERLSTYQVIMGEVPAQQAMLPTEVDNLSLIPAHKNLLGANIELVPLD
                     QREYRLRHALQPLREEFQFIVLDCPPALDLLTLNALVAADTVLIPMQAEYFALEGVTE
                     LLDTIDRIRVELNRGLAVEGVVLTMFDERTNLAQSVASELRGFFGDIMCETAIPRNIR
                     LAEAPSHGQPGLLYDPHARGTEAYLKLAQEILERVTHPQQPSEEAAQPSD"
BASE COUNT         1759 a         2095 c         2270 g         1534 t
ORIGIN      
        1 gcaactgaaa gtaacctgcg ggccggacgc ttcgttccgg cttgcatttc tttgtctgca
       61 aacttgacag cctgatcgcc atgtctgcga gtagcgagac aagaaccctg ccactgcggc
      121 gctggcgctg cggtctgctg ccggaaatct cgcagccacg gactgcaacg gtgtccccgg
      181 gtcggggtaa aatcttgaca gccatggccg aactctttac gcccgcccag cctccggcgc
      241 agcagcgcaa ctggatgccc atcgttgtgg gcctgattgc tgtccttgtg gtcatcggga
      301 tcatcgtcgc ccttacgcgc agccacggac caactggccc ccagaccaat ccttatgcgg
      361 ccaagctaca gctctccaat ccgaagctga gcgccgcgga aaactatgtc ggcggcacgg
      421 tcacctatct cgatgtagac atcgctaaca cgggcgaccg agctttgatt ggcgccgaca
      481 tgaaggtggt gttcaagaac acattggacc aggtggtgca aaccgagacc ctgccgctgc
      541 acgtgctggt ggaaaaccag atgggcggat atcccgacct ggtggacttg ggccgctcgc
      601 cgattggccc aggccagacc aagaccgttc gtatgaccct ggagcacatc tccgccgact
      661 ggaaccggag ctaccctgag attcagctgg tgaatttgaa gctgaagtag ggccaggcgg
      721 tcttttccgg ggcacccgag tcgagggagc tgcggttcga ctgtcccggc aaccgggaac
      781 cccctcccct cgcgcccagc ccggaaggaa agctgctggg aaaaggaaac tcttgctatg
      841 atgcttgctt ggcagtagct tccaagacaa atggctctcc tcaccgaagc ggacacctgc
      901 cgcagatatg tcttacctaa gctgtacgcc gcaggctggt cggacgaaca aataaacgaa
      961 caaaagactt ttacggacgg ccgcatcatc gtcgccggga gcaaggttcg gcgtctcgcg
     1021 caaaagcgtt ccgattatct cttgcgctac cgtcgtgatt ttcccatcgc agtagtcgag
     1081 gcgaagtcca tctacgcgaa acccggcgac ggcctccagc aggccaagga ctacgcacag
     1141 atcttgggtc tgaagttcgc ctacgcgacc aacggacacg gcattatcga gcatgatttc
     1201 ctcaccggac gggacaatga tgttgaaggc ttcccggctc ccgacgagct ctggggacga
     1261 attcgcggaa gcgaaggaat cgatgatgcg accgccgagc gggtgcttgc gccttgttac
     1321 cacctctccg ggaaatctcc ccgctactat caagagatcg caatcaatcg ggccgtgcag
     1381 gcgattcttc agggtaagcg cagggtgctg ctgacccttg ccacgggaac cggcaagaca
     1441 gtcattgcat tccaagtctg ctacaaactg tggaactccc gctggaaccg aaccggcgag
     1501 tatcgccgac cgcgaatcct ttacctcgcc gaccgcaaca ttctcgtgga cgatccaaag
     1561 gacaaaattt ttactccgtt tggcgaggct cgctggaaga tcgagggacg ggccaataag
     1621 ggccgcgaga tgtattttgc gacctaccag gcaatcgcaa aagatgatcg acgccgaggg
     1681 ctgtttcgag aattcccccg cgactttttc gaccttgtgg tcgtggacga gtgtcaccgt
     1741 ggcatcgctc gggacgagag taattggcgc gagatactgg agcacttcga gcccgcgttt
     1801 cagcttggca tgaccgcgac cccgttgcgc gatgacaacc gcgacacgta tgcgtatttc
     1861 ggcaaaccgc tctatctgta cagcttgaaa cagggcatcg aggatgggtt cctcgcgccc
     1921 taccgcgtcc accgcatcct ttcaaccgtc gatgctgccg gttggaggcc gaccaaggga
     1981 gaactcgacc gctacgggag agaaataccg gacggggagt acgagaccaa agactttgaa
     2041 cgcttggttg cactgaaagc gcgtaccgaa gcgattgcaa gaaatctgac cgatttcctg
     2101 aaggccacgg atcgcttcaa taagaccatc gttttttgcg ttgaccagga acacgccgag
     2161 gagatgcgaa aagcgctcaa taattgcaac accgatctcg tcgaacaata tccagactac
     2221 gtcgcgcgcg tggtgtcgga tgaggggaag attggcagag gacacctcga ccgcttcatg
     2281 gagcttgaaa cgctcgttcc cgcaatcgtg acgacttctc agatgctcac caccggcgtg
     2341 gacgtgccca catgtaagaa catcgtgctg gcgcgagtaa tcaactcgat gacggatttc
     2401 aagcagatca tcggacgcgg cactcgcgtg cgcgacgatt atgggaagtt gtatttcaac
     2461 atcctcgact acaccggaag cgcaacccgg ctgtttgccg atcctgaatt cgatggccca
     2521 ccggcacttc ttacagaaga gcaaatggac gcttccggaa ggcagatcga gtccaaggtg
     2581 ctggaagagg aacaggtcga aaacaccgaa gagttggaaa ttcctacaat cgacgaactg
     2641 gacaaaggtg acggagagct gcgccgaaaa tactatgtcg atgaaggaac cgtcaaaatt
     2701 gcggcccacc tggtctacga gctggacgta aacggaaagc aactgcgggt cgtgaagttc
     2761 acggattaca cggcagagaa tctgcgcagc atgtatccct cggcggctgc cctgcgttca
     2821 aaatggagca gcgcccagga acgtgcggaa atcattcagg cactggagga tcgcggcatt
     2881 tcgtttgaaa aactagctga agtggcgaac cagccggacg ccgatccgtt cgatctgctg
     2941 tgtcatgtcg cgttcaacgc tcctttgcgt acacgccggg agcgagcgga gatgctgaag
     3001 cgagaaaaaa cagatttctt tcaacaatat ggacccgatg cgcgcgaggt gctgacccag
     3061 atgctggaaa aatatgtcga gtacggtaca gcccagtttc aaattccaga ggttctctac
     3121 gtcaccccat tctcagatcg ccgttcggta tccgaaattg tagccttgtt tggccgcgcg
     3181 gaccagatgc gcaccgctgt caacgatatg caaacgcttc tatacgcttc ctaaaccatg
     3241 acccgcaaaa cgaaaacaga ggcgccacga tcgaccgcac agcagctcgg gagcatcatc
     3301 aagtcctccc gcgataccat gcgcaaggac aaggggttaa gcggcgacct tgaccggctg
     3361 cccatgctca cctggatcat gttcctgaag tttctggatg acatggaaga cctcagggcg
     3421 gaggaagcga agcttgccgg caagcgcttc cgtcccgcta tcgaaccgcc gtaccgctgg
     3481 cgcgattggg cagcgccgcg agatggcatc ggcatgaccg ggcctgagtt gatcgcattc
     3541 atcaaccagg aggaagccgt acgccccgac gggaagaagg ggcccggctt gtttgcctac
     3601 ttgcgcttgc ttcacagtgc gaacggagac cgccgcgacg ttattgcgaa ggttttcgag
     3661 ggcactgtca atcggatgat taacggttat ctccttcgcg acgcagttaa taaagtgaac
     3721 gaaatccact tcgcctctcg cgacgaaatc cacacgctag ggcaactata cgagagcatg
     3781 ctacgggaga tgcgggatgc tgcgggcgac tcgggcgagt tttatactcc ccgcgccctg
     3841 gtgaagttca ttgtaacggt ggtaaacgct cgcatcggcg aaacggttct ggacccggct
     3901 gccggcaccg gcggctttct tgtcgaagcg ttcgagcacc tcaagaagca gtgcaagaag
     3961 gcagaagact tcacgctctt acaaaaagcg acgcttcacg gcatcgaacc aaagccgctg
     4021 ccgtacctgc tctgccaaat gaatcttctg ctgcacgggg tggagtatcc ggagatcgat
     4081 tcgttaaacg cccttcgttt tccgctgcgc gagatcggag acagggacag agtcgatgtg
     4141 atcatgacca acccgccttt tggtggcgag gaagagcgag gcattctttc gaacttccct
     4201 gaagacaaac agaccgccga gaccgcccta cttttcctcc aactcatcat gcgtaaactg
     4261 aggcgaccgg tgggaggcca caaaggagga aggtgcggca tggtcgtccc gaacggtgtt
     4321 ttattcggtg atggggtgtg tgcccgcata aaggaagagc tacttaagga atttaacctg
     4381 catacaatcg tgcgactgcc aaacggagtg ttcgccccat ataccagtat tccgacaaac
     4441 cttcttttct ttgagcgatc cggaccgacg aaggagattt ggtactacga acatccgctg
     4501 cccgaaggcc ggaagaatta caccaaaacg gcccccgtcc agttcgaaga atttagtgac
     4561 tgcgtgaaat ggtggaagat gcgcgaagaa aacgaccact cttggaaggt ttccgccgct
     4621 gatctgctgg ccgcaggctg taatcttgat cgcaaaaatc cacacgcaaa ggaagacatt
     4681 acccacctgc caccggagca actggctgag agcatcatgc agaaggaaca gcgtatagcg
     4741 gaaatcatgc tgaatatcaa ggagctgtta acaaaatacg actcatgata aaacaggggt
     4801 ggccagctac aagactaggc gaagtgttgc ggcgggtcga gcgattcgaa tctcgcgaca
     4861 acctaaccga gtaccctttc gcgggaacat acagctttgc tcgggggatt ttcgttggtg
     4921 agcgaaagca aggctcaacg ttctcgctgc cgaagatcca gcggattcgg gagggtgact
     4981 ttgtttattg caaaattatg gcgtgggagg gggcgtttgg catcgttccg agggaagctg
     5041 ataactgcgt tttatcggga gccttcgttg tttacgaact aaattctgaa tatatcgatc
     5101 agagctttct tgattacttt tttaaggtgc ctgcgcactg gcagagaatc gggaggcaga
     5161 gttcaggtac taacgtcagg cgacaaagcc tacatcccgt acaatttgag aaagctgaaa
     5221 tccccctgcc gccgctggcg gagcagcgcc ggatcgtaac gcggattgag caagtagccg
     5281 cccaagtcaa cgaagcccgc gctctgcggc agcaagctgt gaacgaggcg gaaggccttt
     5341 gggcgcgcgg cgcagcgcgt accttcgaac aaatcgaaca agcattcccg ctcagatgtg
     5401 tggccgacgt tgtttcagtc cggggtggag gcacgccgtc aaaggcgaat cctttctact
     5461 ggtctggctc tattccttgg gtaacaccca aggacatgaa agtccgcgaa attcacgggg
     5521 cgatcgatca tatttccagt caagcgatta cagaatcttc cgcgaagctt atcgaaccgg
     5581 gtgcagtcct ggttgtggta cggggtatga ttctggcgca cacattccct tcagccattc
     5641 tgcgggttcc cgcgacgatc aaccaagaca tgaaagcact cattcccaaa cccgacgtcc
     5701 ttccggaata tctatgtgcg ttcctttggg cttacaacga tcagattctc ggacgggttg
     5761 agaagtccac acacgatacg cggaaactgg agacgcccgt cttgatgagc atcaaacttc
     5821 cggttccccc tctggcggag caacgccgaa tcgttgctca acttgacgat ctacaaatga
     5881 agacgaatgc cttaatggcc ctacagtctg agacctccgc tgaaattgac gccctcatgc
     5941 cttccattct cgataaagct ctccgaggag agctgtaacg actcgaaggc gtagtgtcga
     6001 gcgcctccgg cccgacacaa agggagcggc gacaagcaga atcctgtacc gtccccgagg
     6061 gactcgggtt cgttaggaac gcttacccag gacttacgtc ctgggctatt cttgtgccgc
     6121 cctgcgggct ggggtctggg caactccgaa agccgtaccg gaatgccact cttttagaag
     6181 tgatctcaca aatcgttttg tatcagggca cggcttcggc cgtgccgcaa tgggtcaata
     6241 acgagttggg cttcagcgcc tgaggggcct gttcttgcaa gggctaaagc cctgaccttt
     6301 cccgctgcgg ccggcacgac taaagtcgtg ccctgataca gcccaaaacg attaatgaga
     6361 tgagtcccgg ttccgcaggc ccatgcccac tcgttatccc acattgtgat atataccaca
     6421 ttgtcgtaca tagcccccat ggtccgcggt taatcgctgg gctgcgcggc ctcttcagac
     6481 ggttgctggg gatgggtgac gcgctcgagg atctcttgcg ccagcttcag gtaggcctcg
     6541 gtaccgcggg cgtggggatc gtaaagcagc ccgggctggc cgtggctggg ggcctcggcg
     6601 agtcgaatgt tgcgggggat cgcggtctcg cacatgatgt cgccgaagaa gccgcgcagc
     6661 tcggaggcca ccgactgcgc caggttggtg cgctcgtcga acatggtgag caccacgccc
     6721 tcgaccgcca ggccgcgatt gagctcgacg cggatgcggt cgatggtgtc gagcaattcg
     6781 gtgacgccct cgagggcgaa gtattcggcc tgcatgggaa tgagaacggt gtcggcggcg
     6841 accagcgcgt tgagcgtcag caggtcgagc gcaggtgggc agtcgagcac gatgaattgg
     6901 aattcttcgc gcagcggttg cagagcgtga cgcaggcgat actcgcgctg atcgagaggt
     6961 acgagctcga tgttggcgcc gagcaggttc ttgtgcgcgg ggatcaggct gaggttgtcg
     7021 acctcggtag gcagcatcgc ctgctgggcc gggacctcgc ccatgatgac ctggtacgtg
     7081 ctgaggcgtt cgggatcgcg gaggaagccg agcccgctgg aggcgttgga ctggggatcg
     7141 cagtcgacca gtagcgtgcg gacgccgagg gaggcgaacg acgcagccag gttgatggcg
     7201 gtcgtggtct tgccgactcc gcccttctgg ttggtgactg cgatgatgcg tcccatagtt
     7261 ttgcaggaaa agactgaact caagagctta gaacgtggcg gggcggggcg gcaatgatgg
     7321 agcgggccgg aagggaagtt ttccgaggaa aagctgtgca agactaggac gggacagcgg
     7381 aaggtgctga ttaggcggag agtaagcggc gagcaaagtt gtgggtaacg gggaggatgg
     7441 gtcgaagagg cagtgttcca cgtggaacat tttcgaggcc caacatcggg cggcgttgcg
     7501 tcgtggaacc tagcgacatt gctggaatgg taaagagtcg aacgtccaac tttaggaagg
     7561 agcttgcgtt gtgggtagct gaggtggagg ggatggacaa ggcagtgttc cacgtggaac
     7621 atttcgaagt taccccatca agtgagctgc ctctttgg
//
LOCUS       DASRKK010000218         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_699995, whole genome shotgun sequence.
ACCESSION   DASRKK010000218 DASRKK010000000
VERSION     DASRKK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699995"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1011)
                     /locus_tag="VFC15_04710"
     CDS             complement(163..1011)
                     /locus_tag="VFC15_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZM09494.1"
                     /translation="MRFSRLRHDCKPKSQTLIRLCCLIGVAFSFTLGAYPQAANNAAA
                     EQRLFSLINQERVKEGLAPLQFDERLAVAARKHTQLMIQDASLSHQVEGEEPLQLRLS
                     DENVRSDHAAENIALDSEDIEVAHVMLMQSPPHRANILGPQFNAVGIGVLKTDDLYYL
                     TEDFANVLPSYSEPEADSAAQQAISDYAKSQGLPEPKRKPQPKLRQMACDMALDDKLD
                     GQKAHDIPSVTSAIAWNASDLEKLPPNLKRLLAQPLTSGYSLGACFAPSVTHPGGVYW
                     LVMVVY"
     gene            1094..2500
                     /gene="rmuC"
                     /locus_tag="VFC15_04715"
     CDS             1094..2500
                     /gene="rmuC"
                     /locus_tag="VFC15_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZM09495.1"
                     /translation="MNIAVVIVLVLAGLVIGAAFAWLAGRSKSAVLAERKSELERDLA
                     AVKSQLAQQQADNSALSAAKAAFEATLESERRSTGEKLRLLSDASEEMKAQFKALAAA
                     ALESSNASFLQLAKGTLEKYQSEARGELEKREKAVETLVKPISDSLKQVDEHVRELEK
                     SRAEAYGNLTSQVALLLKTQEALQTETGNLVKAMREPQARGRWGELQLRRCLELAGML
                     EYCDFLEQVSVTIDERTRRPDVVVKLPGEKNIVIDSKVPLTAYLAALEAQDEATRKDL
                     LTGHARQVRQHIENLSSKSYWTQFESTPEFVVLFLPGEVFFRAAMDADAELIEYGVKR
                     KVIVASPTTLIALLRAVAYGWNQKNLAESAREISTAGKTLYERLTKMAWHFEEIGKRL
                     GGAVDSYNKAVGSMERSVFPIARKLPELDRSLSAADLPDLHQVEKTPRQLESPDWQLQ
                     PDPEPLSLVGEDADPGKS"
     gene            complement(2495..3196)
                     /locus_tag="VFC15_04720"
     CDS             complement(2495..3196)
                     /locus_tag="VFC15_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM09496.1"
                     /translation="MFRGRRDESRLKQQGPPSKALNAEWLHRCCSRALPRLGVELSLQ
                     GEAPSHGLIVSNHLSYLDILAFSAALPCAFVSKAEVASWPIIGRFAEHAGTIFVQRQK
                     QGDGSRANASIAERLRNGVRVVLFPEGTTTNGEQLLRFHSTMLQPAIAVGEIITPCAI
                     RYQLEDGEAENEVCWWGEVPIGPHVLNLLGKRAVQARIIFGEPIPAVGDRKTLGLDLR
                     TRVMALYEQLRSERL"
     gene            3618..3695
                     /locus_tag="VFC15_04725"
     tRNA            3618..3695
                     /locus_tag="VFC15_04725"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3654..3656,aa:Glu,seq:ttc)
BASE COUNT          809 a         1033 c         1087 g          768 t
ORIGIN      
        1 gaatgtgtgg attcatatga tgcaggcggg cgaacagtac attaagtcga acctgaaaac
       61 gaaggcgctg gatttgcctg tcgtcaatcg gcccggcgca catgccgcga cggttcagta
      121 gcaagttggc gagaaacgat ggcaggcatc gtcggattca tatcagtaga ccaccattac
      181 tagccaatac actcctccgg ggtgagtgac gctgggggcg aagcatgccc ccagcgaata
      241 ccccgaagtc aaaggctgcg caagcagcct ttttaagttg ggaggcagtt tttcaagatc
      301 gctcgcattc catgcgattg cgctagtcac gctaggaatg tcgtgtgcct tttgtccgtc
      361 gagcttatcg tccagcgcca tgtcacaggc catttgccgc aatttgggct gaggcttccg
      421 cttcggctcc ggcaggccct gcgatttcgc gtagtcgctg atggcctgtt gggcagcgga
      481 atctgcttcg ggttccgagt agctgggcag aacgttggca aaatcctccg tgaggtaata
      541 aaggtcgtcg gttttgagaa ccccaattcc aaccgcattg aattgcggac cgagaatgtt
      601 cgcgcgatga ggtggcgact gcatcagcat aacgtgggcg acttcgatgt cttcgctgtc
      661 caaggcaatg ttttcggcag cgtgatcgct gcgcacgttt tcatcgctga gccgcaattg
      721 cagcggctct tctccttcaa cctgatgtga tagcgaggcg tcctgaatca tgagttgcgt
      781 gtgtttgcgc gccgcaactg cgagccgctc atcaaactga agaggtgcca gcccttcctt
      841 gactcgttcc tgattgatca gtgaaaacaa ccgttgttcc gcagcggcgt tattggccgc
      901 ttgagggtaa gcgcctagcg tgaagctgaa cgccacgccg atcaggcagc agagtcgaat
      961 caaagtctgc gacttgggct tgcagtcgtg cctgagtctt gagaatcgca tgctaccggt
     1021 tagacgctcc gcgtccgctt ccgttcccat cccggtcaac atacaataca gcacaggttc
     1081 tccgatacat ccgatgaaca ttgcagttgt gattgtcctc gtactcgcgg gtcttgtgat
     1141 cggcgcggcc tttgcatggc tggctggccg atcgaagagt gcggttctgg ccgagcgcaa
     1201 atccgagctg gaacgcgacc tcgctgccgt gaagtcgcaa ctcgcgcagc agcaggcgga
     1261 caactctgct ctcagcgctg ctaaggcggc cttcgaagcg accctggaaa gcgaacgccg
     1321 cagcaccggg gagaagctcc ggctactgtc cgatgccagc gaggaaatga aggcgcagtt
     1381 caaagcgctg gcggcagcag ccctggaaag cagtaacgcc agcttcttgc agcttgccaa
     1441 gggcacgttg gagaagtatc aatccgaggc tcggggagag ctcgaaaaaa gagagaaggc
     1501 ggtcgagacc ttggtcaagc caatttccga ctcgctaaaa caagtggatg agcatgtccg
     1561 cgagttggaa aagtcgaggg ctgaggccta cggcaacctg acttcgcagg tggctttgtt
     1621 gttaaagacg caggaagctt tgcagaccga gaccggcaac ctggtaaagg caatgcgcga
     1681 acctcaggcg cgcgggcggt ggggagagct gcaactgcgc aggtgcctgg agctcgcagg
     1741 gatgctggag tattgcgatt tccttgagca ggtatcggtc accatcgacg agcgcacgag
     1801 gcgtcccgac gtcgtggtca aacttcccgg cgagaagaac atcgtcatcg actcgaaggt
     1861 cccactcacg gcatatctgg cggcgctgga ggcccaggac gaggcgacac gcaaagatct
     1921 tctgacgggc cacgcgcggc aggtccggca gcacatcgag aacctgagtt cgaagtcgta
     1981 ctggacgcag ttcgagtcaa cgcccgagtt tgtggtgctg tttctccccg gcgaagtgtt
     2041 tttccgcgcc gcgatggacg ctgatgctga gctcattgaa tatggagtaa agcgtaaggt
     2101 catcgtggcc tctcccacca cgctgatcgc actgctcaga gcggtcgcgt atgggtggaa
     2161 ccagaagaac ctcgcggaga gcgcgcgaga gatcagcacg gcaggcaaga ccctgtatga
     2221 acgcctgacg aaaatggcct ggcattttga agaaattgga aagcgacttg gcggcgctgt
     2281 cgactcctac aacaaagccg tgggctcaat ggaacggtcc gttttcccaa ttgcgcgcaa
     2341 gctacccgaa ctcgaccgat cgctatcggc agcggacctt ccagacttgc atcaggttga
     2401 gaagacgcca cgtcaactgg agtcgccgga ttggcagctt caaccagatc ccgagccgct
     2461 gtctctggtt ggcgaggatg ctgacccggg aaaatcatag gcgctcgcta cgcaactgtt
     2521 cgtacagagc catgacccgg gtccgcagat ccaaacccag ggtcttacga tcgcccaccg
     2581 ccggtatcgg ctcaccgaag attatccggg cttggaccgc gcgcttacct aacaggttta
     2641 gcacgtgcgg acctatgggc acctctcccc accagcagac ctcgttttcc gcctcgccat
     2701 cttcgagttg gtagcgaatc gcgcacggag taatgatttc tcccacagca atcgccggct
     2761 gcagcatcgt ggagtggaaa cgaagaagtt gttctccgtt ggtggtcgtt ccctcgggaa
     2821 acaagacaac gcgcactcca tttcgcaggc gctcggcgat gctggcatta gcacgcgaac
     2881 catcgccctg cttctgacgt tggacaaaga tcgtacccgc atgttcagcg aatcgtccga
     2941 tgattggcca actagccact tctgccttgg aaacgaaggc gcacggaagg gcagcgctga
     3001 aggccagaat gtcgagatag ctcagatggt tggacacgat gaggccgtga gaaggcgcct
     3061 ctccttgcaa tgacagttca actcctagac gcggcagcgc ccgtgagcag cagcgatgca
     3121 accattctgc attcagcgcc ttcgacggcg ggccttgctg cttgagtcgg gattcgtcgc
     3181 gtcgtccccg aaacacttca cttccaaatg cgagcaggcg cttgagttgt cttgcgcgtg
     3241 tgtcgagcag gttgcctgtt cctccggaaa gcgcgggtgt gcgctccaat ggcagtctaa
     3301 cgaaatcaaa cagtgttcgg agactaatcg tgtggatctc gaaatcgacg acgaccagcg
     3361 catattaccc acttgccaac ttgaaggcgg atagatgaca cgagatgccg tttcctgatc
     3421 tggaaatgag gcttaaacgg caaatgagag ttcgtgcgca atatccgcgg cgccatcctt
     3481 cgagggtgtc aaagtctcgt cgcagttatg gggtaacatt atcttggagc atctgcacct
     3541 cttccgcgta actttgacac ccgttagaac ggactgatat tctagcttgt tcgacgcgaa
     3601 gttccctcca caatagcgtc cccgtcgtct agcctggcct aggacatcgc cctttcacgg
     3661 cgacgacacg ggttcaaatc ccgtcgggga cgccaaa
//
LOCUS       DASRKK010000219         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_601930, whole genome shotgun sequence.
ACCESSION   DASRKK010000219 DASRKK010000000
VERSION     DASRKK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601930"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFC15_04730"
     CDS             <1..1726
                     /locus_tag="VFC15_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09497.1"
                     /translation="ADNVESMSVYTAGIPAEYGRKMGGVVEVNTARDSREGFHGQVVL
                     SGGSFDTAGAYTMLQYLSGKNLFGISASAAMTSYYLSPPVPQNFTNTGTTGDFAGRYE
                     RDFTPSDRLTLMFRHELARYDIPNEQVQQAAGQRQDGDNFENMGLATYRHIFSSNVVG
                     NFGAMVRDNSNGLSSNLLSTPIIAFQRNHFTEAYFKGTISIHHGNQEWKAGVESDNLF
                     LHEHFADVITDPSQFDPGTPTRFSFVGNRPDLEQSAFVQDLIRLGNWTVSAGVRWDHY
                     QLIVNQNAVSPRLGVSRYFAKSQLLVHAAYDRVFQTPSFENILLSSSPDVLVLNPNVL
                     RLPVEPSHGNYYEFGVTKGFMGKFSLDVNSYFRDVNNFADDDLLLNTPVSFPIAFRKA
                     QIYGAEGKVNLPLWGHLSGFVSYSYMVGSAYFPVTGGLFLGQSATNALTNLAGRFWVS
                     QDQRNTVRTRFRYQFLPRFWAALGGEYGSGLPVDFDGTYEQALAQYGQQVIDRVNFKR
                     SRVKPSFSLDASIGADIWKKDTLAMRVQFDVENINDGLNLINFAGLFSGNSIAPPRSY
                     AVRLKAEF"
     gene            complement(1904..>2402)
                     /locus_tag="VFC15_04735"
     CDS             complement(1904..>2402)
                     /locus_tag="VFC15_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09498.1"
                     /translation="IPQKFSYGNYIEAMTLVRTEVLKELGGYRQMPNKFGWEDYDLWC
                     SFVDRGLKGCHVPQILCRYRVHKGSMIRNVTNAFVESEMEKVRHDFEAHHNMPFYFLS
                     GPRRRHQILRRALSPSGAEERVRVARALCSRLPLPKRTRHSLLVWILSHLRALERRRI
                     QCIGL"
BASE COUNT          532 a          679 c          643 g          548 t
ORIGIN      
        1 agcggacaac gtcgagtcga tgagcgttta caccgccggc attccggctg agtacggacg
       61 gaagatgggc ggcgtcgtcg aggtcaacac cgcgagagac agccgggaag gtttccacgg
      121 ccaagtggtg ttgtcaggag gaagcttcga tactgccggc gcctacacca tgctgcagta
      181 tctgtccggg aagaacctgt tcggcatcag tgccagcgcg gcgatgacaa gttactatct
      241 cagtcctccc gttccccaaa acttcaccaa cactggaact acgggggatt tcgccggcag
      301 atacgagcgg gacttcaccc cgagcgaccg cctgacttta atgtttcgcc acgagctggc
      361 tcgctacgac atacccaacg agcaggtcca gcaagctgca ggccaacgac aggatggcga
      421 caacttcgag aacatggggc tcgccaccta tcggcatatt ttctcttcga atgttgtggg
      481 caatttcgga gcaatggttc gagacaattc gaacggcctc tcatcgaatt tgctgtcgac
      541 gcccatcatc gccttccagc gcaaccactt tacggaggcg tacttcaaag ggacgatttc
      601 cattcatcac ggcaatcagg agtggaaggc cggtgtcgaa tcggacaacc tcttcctgca
      661 cgaacacttt gccgacgtaa tcaccgaccc ctcgcagttc gacccgggta cgccgaccag
      721 gttcagcttt gtgggcaatc ggccggactt ggagcagtcc gctttcgttc aggacttgat
      781 ccgtctcggt aattggaccg tcagcgcagg cgttcgctgg gaccactacc agctcatcgt
      841 caatcaaaat gcggtgagtc ctcgcttggg agtttcgcgc tacttcgcca agagtcaact
      901 cctggtccat gccgcttatg accgggtttt tcaaactccc tcttttgaaa acatcctgct
      961 gtcgagttcc cccgacgttc tggttctgaa ccccaacgtg ctgcgcctgc cggtagagcc
     1021 gtcgcatggg aactactacg aatttggcgt gacgaaaggg ttcatgggga aattcagcct
     1081 ggacgtaaat tcgtatttcc gcgacgtgaa caatttcgca gacgacgacc tgctgctcaa
     1141 tactccggtc agcttcccta tcgcctttcg caaagcccag atctatggag ccgaagggaa
     1201 agttaacctt ccgctctggg gccatttgtc agggtttgtg agctattcct atatggtggg
     1261 ctcggcctat ttcccggtca cgggaggtct ctttctaggg cagtcggcga cgaatgctct
     1321 gacaaatcta gctgggcgtt tctgggtctc tcaggaccag cgcaacacgg tgcggacgcg
     1381 cttccgctac cagttcctgc ctcggttctg ggctgcactt ggcggagaat atgggagcgg
     1441 cctgccggtc gatttcgacg gcacgtacga acaggcgctc gcgcaatacg gtcagcaagt
     1501 gatcgatcgc gttaatttca agcgcagccg agttaagcct tcgttctcgc tggatgcttc
     1561 catcggagcg gatatctgga aaaaagacac ccttgctatg cgggtgcaat tcgacgttga
     1621 gaacatcaac gatgggctca atctcataaa ctttgcggga ctgttctccg gtaactccat
     1681 tgcgcccccg cgcagctatg cagtccgtct caaagcggag ttctaaagaa actctgagca
     1741 gatcgatttt ggtagaggcg gctttcgatc cgcgcaactc gaccatcatc tgcgcggcgc
     1801 agatgtgaga tggccgggcg cataacatcg ctgcagtgcg gcggtccact tattccgaat
     1861 gcagatatcg aaagcgtctc gtcgcataga agccggcaag acttcacaat ccgatacact
     1921 gtattcttct gcgctcaagc gctctcaaat gactcagaat ccataccagc aatgagtgtc
     1981 tggttcgttt ggggagaggc aatcgggagc acagcgcccg agcaacacga acacgctcct
     2041 cagcgccaga agggcttaat gcccgcctaa ggatctggtg gcgacgacgg ggtccggaga
     2101 gaaagtaaaa aggcatgtta tggtgtgctt cgaaatcatg gcggaccttc tccatctcgc
     2161 tctcgacgaa agcattggtt acgtttcgaa tcatagagcc tttgtgtact ctgtagcggc
     2221 agagaatctg cggcacatgg catcctttaa ggccacgatc gacaaatgag caccataggt
     2281 catagtcttc ccatccgaac ttgtttggca tctgccgata cccaccaagt tctttcagga
     2341 cttccgttct caccagcgtc atcgcctcaa tatagtttcc ataactgaat ttctgcggta
     2401 tc
//
LOCUS       DASRKK010000220         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1188701, whole genome shotgun sequence.
ACCESSION   DASRKK010000220 DASRKK010000000
VERSION     DASRKK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188701"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFC15_04740"
     CDS             <1..576
                     /locus_tag="VFC15_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM09499.1"
                     /translation="QYVLPAASIAASPRPASEATAIAVGLWGAAIVSAGMALSMLVTL
                     NGTIMSGARIPYAMARDGYFFKAMADVHPRFHTPSVALIVQLALSIVLLLFGGNFRQL
                     FSLTIFAEWLFYMIAASTVFVLRWKEPNAPRPYKTWGYPIVPAVFVVVAAMLLYFTFT
                     ENLRNSIWGCGLILSGIPVFAWFARKRARAE"
     gene            2177..>3141
                     /locus_tag="VFC15_04745"
     CDS             2177..>3141
                     /locus_tag="VFC15_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HZM09500.1"
                     /translation="MRPRRFACVIAIVLAYTVLATQSFACTAFQLTSGDGSKIYFRSM
                     EFGFPFHSNVLIIPRGAQYSGATPDGKPGLKWSAKYGAVGLNAEVAPDCLTDGMNEKG
                     LVVGMLYLPGDGQFLPPDPSKDDRTIGGWQAAAYLLTTVASVEEAKQVLTSEKVYIAE
                     QIFPPFKAQLPVHFYIGDASGAVVIAEYVNGKLNLHDDPLGVLTNSPPFNWQMTNLSN
                     YVNLSPVNVPELNLANNVDIQNFGQGSGMLGIPGDYTPPSRFVRVALFSHWVSAGKTV
                     SETVNAGFHVLNTFDIFKGAVRSSKTSGSEMDVTEWAVAHDRTNL"
BASE COUNT          618 a          852 c          924 g          747 t
ORIGIN      
        1 cagtacgtcc tgccggcagc cagcatcgcg gcctcgccgc gtccagcttc ggaggcaacc
       61 gccattgcgg tcgggctgtg gggagcggcc atcgtctccg ccggaatggc gttgtcgatg
      121 ctggtcacgc tgaatggaac gatcatgagc ggcgcgcgca ttccctacgc catggcgcgt
      181 gacggatact tcttcaaggc catggcagac gtccatccgc gtttccacac accttctgtg
      241 gcgctgatcg tgcaacttgc gttgtcgatt gtgttgctgt tgttcggcgg aaacttccgc
      301 cagttgtttt cgctgacgat ttttgccgag tggctgttct acatgatcgc tgccagcacg
      361 gtgtttgtac tgcgttggaa agagccgaac gcccccaggc cttataagac atggggctat
      421 ccgattgtgc cggctgtgtt cgtcgtggtt gcggccatgc tgttgtactt cactttcacc
      481 gagaacctgc ggaactcgat atggggttgc gggctgattc tttccggtat cccggtcttc
      541 gcctggttcg cccggaaacg ggcccgagcc gagtaggagg aggtacccct acccccgatg
      601 cctcaaaata cttgttttca gcgagataga cgaattctcg cgtaaattat tgacggcaaa
      661 taacttacac gtaaagtctt cttctgtaag gacttgcggg tagaacttta cccgtggaga
      721 gccgcgggag gggaggaaac ggtaagagcg gtaggacatg gcaccaccaa aaaaagcagc
      781 tgtcagcggt tcgcacagcc aacagtaagt tgttacaggc tgccggtgga ttggcagcaa
      841 agcactgtaa tgcccgacgg gggatttctc cctcggtgta cgagtggatg atggtgcgga
      901 ggttgtcaac ttacgaaggt actcaactgg ggcaaacttt cataaccaga tactgttggc
      961 ggcagtcccg caggtgggag gcgtttgggc tcagcaatgc cagacgtcgc tggcggctcg
     1021 tagttattgc gcagttcctc agcgttgtcc tgagcctttt ttgagcccgt gctaccatgc
     1081 caccgtgttc aattttggca aaccgaaaac agtgggcgac tggatcgtac atgtcgcggg
     1141 tgccgtcgtc gcattactcc tcgtatggtg gatgctgcgg agctatgtcc tgtaatcccc
     1201 gcttgcttga attacgaaat aacgatttcg tcctttccgg gtgtgttgct cttttgccac
     1261 tgtcaccggt gctgaaggct gcccgcccgt gacccatagt tggcgataaa tcgccgactc
     1321 gtaggtaatc cctgctgagc gccgccagtc ggtttaacgt acggcctgat atcgccaacg
     1381 atccttgcga gaaacaatct gccggttcag gaggagttgt cgcagcggat gtcggacgcc
     1441 aggtcgggtg tatagtgaag ggccattgag agcttccggt cggttttcct agggagattt
     1501 cagatgcatt tttcgaggca gattgtgctg ggcatagcaa ctggcgcgac tctggtttcg
     1561 cttggcttcg tcatggtgag ctattcggtt gaccaatccc acatgacact cttccttcgc
     1621 tatctctgga tggttctata ttttcttttc gccatggcag tcatgctttg cgtggacgtg
     1681 gcgttccaag ttgcaaccgg cggatggtgg tcaccatcca gggctgtacg atactccaag
     1741 gttatcgggc gttgggcgac aagcgccctt gtgatatggc ttgcgttttg gcacgaacac
     1801 gtctttttgt ggattgtttt cgcgctcgct ctggttgtcc tcttttggat tcgattcagg
     1861 gatcattcca tggcggttag tcaatgaacc aaataggctt gtctagcgat tactgccgat
     1921 aaatcgccat ggcggaggta atcgcggctg agcgccgccg tttcggttaa cctcggacat
     1981 gatggcctga tatcgcacca gctatcattg gacagccacc tcccgagtcc cacatcatgg
     2041 cccgagactg caaaagtcac caatggtaat ccgatgtgtg gggcgagaac cgacgtctca
     2101 cactcatctg gtataggggt cacagacatg cccgtcgtgg cgggttacgg tgactgaagt
     2161 gtaaggaggg cccttcatga gaccccgccg ttttgcttgc gttatcgcaa ttgtcctcgc
     2221 ctataccgtg ctagccacgc aatcgtttgc ctgcactgct tttcaactca catccggcga
     2281 cggatcgaag atctacttcc ggtccatgga gttcggcttc ccattccatt cgaacgtact
     2341 catcatcccg cgcggcgcac agtacagcgg cgcgactccc gacggaaaac cgggattgaa
     2401 gtggagcgcg aagtatggtg ccgttggact caatgctgaa gtcgctcccg actgccttac
     2461 ggacggcatg aacgagaagg ggctcgtcgt cggtatgctg tacctgcccg gcgacggtca
     2521 gttcctgccg ccggatccgt cgaaggacga tcgcaccatc ggaggctggc aagccgccgc
     2581 gtacctgctc accacggtcg cctcggtgga agaggccaag caggtcctga cgagcgagaa
     2641 agtctacatt gcggagcaga tctttccgcc gttcaaggcg caactgccgg tgcacttcta
     2701 tatcggggat gcttccggcg cggtcgtcat tgcggaatac gtgaatggca agctcaacct
     2761 gcacgacgat ccgctgggcg tgctgaccaa ctcgccgccc ttcaactggc agatgacgaa
     2821 cctgtcaaac tacgtgaatc tctcaccggt gaatgtgccg gagctgaatc tggccaacaa
     2881 cgtggacatc cagaactttg ggcagggcag cggcatgttg ggaatccccg gcgattacac
     2941 tccgccctcg cgcttcgtgc gcgtagcgct gttctcgcac tgggtgagcg cgggcaagac
     3001 cgtgagtgag acggtgaatg ccggcttcca cgtgttaaat acgttcgata ttttcaaggg
     3061 agcagttcgc agcagtaaga cctctggcag cgagatggat gtaacagaat gggcggtggc
     3121 gcacgaccgc acgaacctga a
//
LOCUS       DASRKK010000221         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_16095, whole genome shotgun sequence.
ACCESSION   DASRKK010000221 DASRKK010000000
VERSION     DASRKK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16095"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1201)
                     /locus_tag="VFC15_04750"
     CDS             complement(92..1201)
                     /locus_tag="VFC15_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZM09501.1"
                     /translation="MKKNIEISPNIETLFGTRDENLRLLEDGLNVAIAMRPDSIEIEG
                     AASDVTRAQQVFADYQHLLRNGFTFVNGDLGAMIRVLTSDPTATLRGLAEAGKQRSFG
                     KRSVQPKSINQRRYLEAIENSDMVFGVGPAGTGKTYLAVAMGIAALMSKRVNRIVLAR
                     PAVEAGERLGFLPGTLQEKVDPYLRPLYDALYDMLEPDKVDRYLEKNIIEIAPIAFMR
                     GRTLNDAFVILDEAQNTTSEQMKMFVTRLGFNSKAVITGDITQIDLPNASRSGMVEAI
                     EILRNVEGIRFCYFDEGDVVRHHLVQRIIRAYDDYKGRNEQLSLLLEIKNGNGFATAR
                     SNDGRAPTTSVERETSLAEVPSAEAPPAPQNRVSD"
     gene            1586..1978
                     /locus_tag="VFC15_04755"
     CDS             1586..1978
                     /locus_tag="VFC15_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09502.1"
                     /translation="MQTYSLVVFQPRPALFRNMTVTVQAEFRLFGTLKREGEWYIAHC
                     PPLDITTQGRTEEEAKKNLIEASELFVVSCFERGTFEQALRELGWRVVTGGAVAKVGD
                     GPPSVPAGSFAFPIPVPFGFESPAGCHA"
BASE COUNT          422 a          587 c          602 g          464 t
ORIGIN      
        1 tcccttcggt ttcgtgctcc agaatgatca cttccgtttc aagtacaaat gggagggcac
       61 atgagccctc ccatatcatg gtagcaagaa tttagtcgga aacgcgattt tgtggcgctg
      121 gaggcgcttc agccgaaggt acctccgcca acgatgtctc ccgctcgacg ctggtcgtag
      181 gcgcccgtcc atcgttgctg cgcgcggtgg cgaagccatt accgttcttg atttccagca
      241 acagcgacaa ctgttcgttg cgccccttat agtcgtcgta cgcccggatg attcgctgca
      301 ccaggtgatg gcgcaccacg tctccctcat cgaagtagca gaacctgatc ccttccacgt
      361 tgcgcaggat ctcgattgcc tccaccattc cgctccggct ggcgttcggc agatcgatct
      421 gggtaatgtc accggtgatg accgccttgg agttgaagcc gaggcgggtc acgaacatct
      481 tcatctgctc cgaggtggtg ttctgcgctt catcgagaat gacaaacgca tcgttcagcg
      541 tgcgtccgcg cataaacgcg atgggagcga tctcaataat gttcttctcc aggtagcgat
      601 ccaccttgtc gggttcgagc atgtcgtaca aggcgtcata caaaggacgc aggtacgggt
      661 cgactttttc ctgcaacgtt ccgggcagga agcccaggcg ctcgccggct tccacggcag
      721 gccgcgccag cacgatgcga ttcacgcgct tcgacatcag cgccgcgatg cccatcgcca
      781 cggcgagata agtcttcccg gttcccgcgg ggccgacgcc gaataccatg tcactgtttt
      841 cgatcgcctc gaggtagcgc cgctggttga tgctcttggg ctgcacgcta cgcttgccaa
      901 acgagcgctg tttgccggct tcggcgaggc cgcgcaaggt ggctgtcgga tcggaggtca
      961 agacacgaat catggcgccg agatcgccgt tcacgaaagt gaaaccattc cgtagcaggt
     1021 gctggtagtc ggcgaacacc tgctgcgcgc gggtcacgtc gctggccgct ccctcaatct
     1081 cgatggagtc cggccgcatg gcgatggcga cattcaaacc atcttccagc aggcgcaggt
     1141 tttcgtcacg ggtgccaaat aaggtttcga tgttcgggct tatttcgatg ttctttttca
     1201 tctagagtcg gaagtcagcc tccgtatgta tcgggcaccc aactcgtgcc ccgccgtggc
     1261 gggcggattg aggtggttga aagtcttggt tacgaaagcg ggatgggccg gtgacagacc
     1321 aaccgcttcg gtctgacgtt ctgatgcatg gcaagggtat gcgatggtcg ggattgagcc
     1381 cggaagatca cccacgggaa aaacaactct cattggccgc tagcctatcc ccgaatcggg
     1441 acagagtcaa atcaataaaa cgcaactcga tgacgatccg aattcgctct ctgcaactct
     1501 gccgcgcatt ccccggttat ttagatggta ccaggaagaa aagggagcac cgtaccgcgc
     1561 atcttgtatt attgatagag catctgtgca gacgtacagt ttagtggtat ttcaaccgcg
     1621 ccctgctctg tttcgcaata tgacggtcac tgttcaagca gaattcaggc tgttcggcac
     1681 tctcaaacga gagggggaat ggtatatcgc ccattgcccg ccgcttgaca tcaccactca
     1741 gggccggacc gaagaagaag ctaagaaaaa cctgatcgag gcgtctgaat tgttcgtggt
     1801 cagttgcttc gagaggggaa cgttcgagca ggctcttcgc gagctgggat ggcgcgtcgt
     1861 gaccggcggt gcggttgcga aggtcggaga tggcccccct tcagtgcctg cgggttcgtt
     1921 tgcattcccg attccagttc cttttggctt tgaaagtccc gcaggatgcc acgcctgacc
     1981 ccggttccgt gggaaaggct ggtctgcgtt tttgaacaac tcggatatca ccgcgctggc
     2041 caaaagggaa gtcacataaa actggagaag ccggg
//
LOCUS       DASRKK010000222         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_602196, whole genome shotgun sequence.
ACCESSION   DASRKK010000222 DASRKK010000000
VERSION     DASRKK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602196"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFC15_04760"
     CDS             <1..345
                     /locus_tag="VFC15_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZM09503.1"
                     /translation="ALSDRSHPQPRVINTDLAPIYSSAIPDSKKEGTLRTRCRHRPVQ
                     YLNNILEQDHRAIKRQVNAKQGFREFQAARRTIQGYEAVHMIRKGQARWVAGDDLLRQ
                     IQFIDSLFDLAA"
     gene            complement(474..1946)
                     /locus_tag="VFC15_04765"
     CDS             complement(474..1946)
                     /locus_tag="VFC15_04765"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM09504.1"
                     /translation="MGADHRPLVLLTGASGYIGGRLLKELEKAGWPVRCLARRPDFLR
                     PKVAASTEVVKADCLDRSSLAPAMAGVHTAYYLVHSMGSPGKFEQEDREAAQNFADAA
                     RDSGVRRIVYLGGLGNQGQALSAHLRSRHEVADILRSSEIPTIEFRASIVIGSGSLSF
                     EMIRALVQRLPVMICPRWVAVKAQPIAVEDVVAYLMAALVLPVEQCGVFEIGGTDQVS
                     YGQIMQEYARQCGLRRWMIPVPILTPRLSSLWLGLITPVYARIGRKLIDSMRNPTLVE
                     DSSALTAFDIKPKSLREAMERALHHEDQEFAQTSWCDALSSAGKPQSWGGIKFGTRLV
                     DSRTVQVSVPPASAFAPIQRIGGSNGWYFANVLWWFRGLLDLLVGGVGLRRGRRDPHT
                     LSAGDALDFWRVETFVPDRELGLVAEMKVPGRAWLQFEVEPNSRGSVIRQTAIFDPAG
                     LAGLLYWYMLYPVHRWIFSGMLNEIAAVAGRKTSISSLGA"
     gene            complement(2296..2646)
                     /locus_tag="VFC15_04770"
     CDS             complement(2296..2646)
                     /locus_tag="VFC15_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09505.1"
                     /translation="MNLRKGRCRSRSDRNASAFAPWLGLCGENKISGGKVLYTKSRRV
                     RSRVATALRMAANSLHHAKNYLGEFFRRITRKLGKPQAITATAHKLARIVYHLLSTKE
                     AYDEPSFTAAMRRL"
BASE COUNT          607 a          787 c          730 g          546 t
ORIGIN      
        1 gccctcagcg atcgatccca tccccaacct cgagtgatca acaccgacct ggcaccgatt
       61 tacagctctg cgatccccga cagtaagaag gaagggacgc tgcgcacccg ctgtcgacac
      121 cgacctgtgc agtacttgaa caacattctc gaacaggatc atcgagccat caaacgtcaa
      181 gtgaacgcca agcagggctt ccgagaattc caggcagctc ggcgaacgat tcaaggctac
      241 gaagcagtcc acatgatccg gaaggggcaa gctcgatggg tggcaggaga tgatcttctt
      301 cgtcagattc agttcatcga cagccttttc gatttggcag cctaagaacg tgcggcagac
      361 gctcggcgac aaacttgtct gcatttgaaa cttgcaacac tacgttcacg tccggatctg
      421 agagagcgcg gtggtgaaac tccgccgcgc cactctaccg tggaaactga ctgtcatgcc
      481 ccgagcgagg aaatactcgt cttccgtcct gcgacagctg caatctcgtt gagcatgccg
      541 ctgaaaatcc agcgatgcac agggtaaagc atgtaccaat agagaagtcc tgccaatccg
      601 gcaggatcaa aaatagcggt ctgccgaata acggagcctc ggctatttgg ttccacctcg
      661 aactgcagcc acgcgcggcc cggcactttc atttcagcga ctagaccgag ttcgcgatcc
      721 ggcacaaagg tttccactcg ccagaaatcc agggcatcgc ctgcggaaag cgtgtgagga
      781 tcgcgacggc cccggcgcag tcccacgccg cctacaagga gatctaagag gcctcgaaac
      841 caccaaagaa cattggcaaa gtaccagccg ttcgaacctc cgatccgctg aatcggagca
      901 aaagctgaag ctgggggaac cgatacttgc actgttcgag aatcgacgag cctggtgcca
      961 aactttatgc caccccatga ctgcggcttc cccgccgagg agagagcgtc gcaccaacta
     1021 gtctgggcaa attcctggtc ttcatggtga agagctctct ccatcgcttc tcgcaaactt
     1081 ttcggcttga tgtcaaaggc cgtcaaagcg gagctatctt ccaccagagt cgggttgcgc
     1141 atactatcga tgagcttgcg gccgatgcgg gcataaacag gggtgattaa gcccaaccac
     1201 aaacttgaaa ggcgaggcgt caagatcggg accgggatca tccatcgccg gaggccgcat
     1261 tgtcgggcat actcctgcat gatctggccg tagctgactt ggtcggtgcc cccgatttcg
     1321 aaaactccgc actgttccac cggcaacacc agcgcggcca tcaggtacgc aaccacatct
     1381 tcgaccgcga tcggttgggc tttgacggca acccagcgcg ggcagatcat caccggcaac
     1441 cgctgcacca gcgcgcgaat catttcaaat gaaagactgc cggaaccgat cacgatagat
     1501 gcgcgaaatt cgatggtggg gatctctgag cttcgcagga tgtctgcaac ctcgtgacga
     1561 ctgcgcaaat gagcggagag cgcttggccc tggttcccca agccgccaag ataaacaatg
     1621 cgtcgtactc cagaatcgcg tgctgcgtcg gcgaaatttt gggcggcttc gcggtcttct
     1681 tgttcgaact tacccggtga tcccatggag tggaccaggt aatacgccgt gtgtactcct
     1741 gccatcgccg gcgctagtga actacgatcc aggcaatctg ctttcacgac ttccgtggaa
     1801 gcagcaactt ttggtctgag aaaatcaggg cgtcgagcta ggcaacgaac gggccagccc
     1861 gctttctcca gctcctttag taggcgcccg ccgatgtagc ctgacgctcc cgttaacagg
     1921 acgagcgggc gatgatctgc gcccattaga cctcctcatt cggcccattc tgtcaggctg
     1981 tgctgatcct agcatttcct ggatcacttg tttttcacga aaagccagtg gccaaacact
     2041 tgcaagctgt ggtttctgct cggtcggccg tcctaaaaac gccttcctta atctactagc
     2101 gggccttcgc gcgattgtgc cagagtcacg ggcaagccga agctaatgcc tccggattgg
     2161 tcccgataaa cgcccaagca tccaggatcc tgtctccagc ctagccgact cccaaggaac
     2221 atcacccgtt ctgtaggggg atgatttgaa acccgagttg agcggcttgt cggcggagcc
     2281 gaagttgcgc gcgtcttaga gcctcctcat cgcagcggtg aaagacggtt cgtcgtaggc
     2341 ttctttcgtg ctgagcaggt gatacacaat gcgtgcgagt ttgtgagccg tggccgtaat
     2401 tgcttggggt ttgccgagct tgcgagtgat gcgccgaaag aactcgccga gatagttctt
     2461 ggcgtgatgc agcgagttcg cggccatgcg cagcgcggtg gcaacgcgac tcctgacgcg
     2521 gcgactcttg gtatagagca ccttgccacc actgatcttg ttctccccac agagtcccaa
     2581 ccaaggggca aaggcggaag catttcgatc tgatcgactg cgacaacgac cctttcttag
     2641 attaatgtcg gagcgtggct agatccgccc
//
LOCUS       DASRKK010000223         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_602302, whole genome shotgun sequence.
ACCESSION   DASRKK010000223 DASRKK010000000
VERSION     DASRKK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602302"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFC15_04775"
     CDS             complement(<1..843)
                     /locus_tag="VFC15_04775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM09506.1"
                     /translation="MPADPTFSHRVGHSALELHEGTAESVAAIYNQAGDDYVAYADGD
                     PTQLFAFDGMHAYADRRVWTLLETKLADLRASGASSVSILDAGCGPGTWLRRLVTRAR
                     ALGFTSITARGFDIAHAQIQRARLRARNLSRLPGVNFTFDVADLMGRLPESDASVDIT
                     LCLYSVLSHLPVASLTDISTEIARVTSGYFITTVRSIGSMPTAFVDSIEKVRQLKQDH
                     ARNRCEIEFCDGRRIAFSFHLFTASELRSYFASSFDIEDLRGLDLFHSRFIPDPRWNP
                     ACSRG"
     gene            complement(896..1303)
                     /locus_tag="VFC15_04780"
     CDS             complement(896..1303)
                     /locus_tag="VFC15_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09507.1"
                     /translation="MDAMIQLARAAKSHRMIAAGPYSAEVFLELHRRGYPRVTTTKTS
                     RVPCGQYDVALVAWRAHSIKALETTLDWLVHFLSAAGVLVIWVGPHERMPNQTLSLAL
                     KRLGFRIESGTSCENGIAIAARRLDSVPAAKAA"
     gene            complement(1399..1689)
                     /locus_tag="VFC15_04785"
     CDS             complement(1399..1689)
                     /locus_tag="VFC15_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09508.1"
                     /translation="MKHTEPPSIQSCTRPSLFLIGKDSRGNWVVQEEQGNCGGLFVNR
                     AEALKFAKFENGNRPQAVIMVPGIFELDTSGKTALAHHLSHNTETPRERRVA"
BASE COUNT          479 a          594 c          652 g          459 t
ORIGIN      
        1 gcctcgcgaa catgcagggt tccaccgagg gtcgggtatg aatcgactat gaaacaggtc
       61 gagacctcgc aaatcttcga tgtcgaaact gctcgcgaaa tagctccgca gttcggatgc
      121 agtaaatagg tgaaagctaa atgcaatacg tcggccgtcg cagaactcga tttcgcaacg
      181 gtttcgggcg tgatcttgtt tcaattggcg gaccttctca atcgaatcta cgaaggccgt
      241 tggcatgctg ccaatcgaac gcacggtagt tataaaatat ccggaagtaa cacgtgcgat
      301 ctctgttgag atatccgtaa ggcttgcaac aggtagatga ctgaggacgc tgtagaggca
      361 aagagttatg tccaccgagg catcggattc aggcaaccgg cccataaggt ctgcgacatc
      421 aaatgtgaaa ttcactccag gaagcctgga aagattccgg gcccgcaacc gagcgcgttg
      481 aatttgcgca tgcgcaatgt caaagccgcg cgcggtgatg ctggtgaacc caagcgcgcg
      541 tgcgcgtgta accaaccggc gcagccatgt gccgggaccg caacccgcat ccagaatgct
      601 gaccgagctt gccccggaag cgcgcaggtc agccaacttt gtctccagaa gagtccagac
      661 gcgccgatcc gcatatgcgt gcattccgtc aaaggcaaac agttgcgtcg gatcgccgtc
      721 agcataagcg acgtaatcgt caccagcctg gttgtagata gccgcgacgc tctcggccgt
      781 tccctcatga agctccaggg cagaatgccc aactctgtgt gagaacgtcg gatcggcggg
      841 cacgtagtct gtcagttgcg aagcgtttcg accgatggcg gacattgtcg gagccttatg
      901 cggccttggc ggcgggaacc gaatcaagcc ggcgcgcggc gatggcaatg ccgttctcgc
      961 agctggtgcc cgattcaatg cgaaagccca atcttttcag cgccagactg agcgtctgat
     1021 tgggcatgcg ttcgtggggg ccgacccaaa tgaccaaaac gccagcagca cttaggaagt
     1081 gcacgagcca gtcgagggtc gtttcgagag ctttgatcga gtgcgcccgc caggcgacaa
     1141 gagcgacgtc gtattggccg cacggaacac gactagtttt agtggttgtc acacgtgggt
     1201 agccgcggcg atgcaactcc aggaagacct cagcgctata ggggcccgcc gcgatcattc
     1261 gatgcgattt cgccgcgcgc gcgagctgga tcatggcgtc cacgatccgc tcatccgagg
     1321 gaatgggact ggattgggtc atgtgtaccc cccaaacggg gacgatggtt ggattggatg
     1381 agaacgaccg caggcccttc aggccacccg tcgctcgcgt ggagtttcgg tattgtgcga
     1441 cagatgatgt gctagggccg tcttgccgct cgtatcgagc tcgaaaatac cgggaaccat
     1501 aataacggct tgtggacgat ttccattctc aaactttgcg aacttgaggg cttcggcgcg
     1561 attgacgaaa agtccgccgc agttgccctg ttcttcctgg accacccagt tgccccggct
     1621 gtccttgccg atcaggaaga gcgaaggtct cgtacacgac tgtatcgagg gaggctcagt
     1681 atgtttcatg gcacgtctcc ttgatgttga atggcgtcag gttgctttca gaaggctcac
     1741 ttagtggaga tacatgggaa gccagattgc cagtcgaatt gcgatcgttg cggcaattgc
     1801 ggcggcgcaa agtgcgaata cgagaacaat cggataggcg cgccttgcca tcgcgacagt
     1861 ccatcgtgcc gcgctcagcc acaccatttc cagggacgag agcccggatg atgaccggct
     1921 caggatcttc tgttgaacag cgtgagcgcg tgaagtgggc gacatgtcag atattctggc
     1981 cgctgattga gttcgttcca tttgcaaatt ccatcgctac gacatgctct cggttcggag
     2041 gcctcagcgg acgatcggga ggtcgtcgtc gttcttcgat gcgtgcttgt aacggccgcg
     2101 gaataactcc ctttcgattg ccgcccgatg tatgcgtttc tcggcaagag cacgtgagaa
     2161 ccgtgcaatg gtcatccgtc ctgc
//
LOCUS       DASRKK010000224         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_798279, whole genome shotgun sequence.
ACCESSION   DASRKK010000224 DASRKK010000000
VERSION     DASRKK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798279"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..1643)
                     /locus_tag="VFC15_04790"
     CDS             complement(564..1643)
                     /locus_tag="VFC15_04790"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZM09509.1"
                     /translation="MFLHGIFPPITTPFYPDGKIYFRKLEQNVEHYSLTPVVGIVVLG
                     STGEAILLSDEERREVLKCARESAANEKVLVAGTGIESAIETLRLTEYAAELGYDIAM
                     VRTPHYYKGQMTPANMLAFYRFVADRSPLPAIIYNFPQATGYDIPSEVVVELADHPNL
                     IGIKESSGSVEKVQKMVEATRHIKRSATVTETFAAVTGRMLHAATAASASAEGGALVT
                     VGALAGTAAPKPSSVSVKVVGGLKTRQKEVGFQVLVGAAQKLHPSLDAGAVGAILAFA
                     DCAPTACYEIYAAWKDGDAALAREKQERIAKAAERIVGALSIPGVKYAMDFNGCFGGV
                     PRLPLLPLTADKKTEVEKLLENIRN"
     gene            complement(1678..2772)
                     /locus_tag="VFC15_04795"
     CDS             complement(1678..2772)
                     /locus_tag="VFC15_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZM09510.1"
                     /translation="MNRDSKFELPVGIERYLSALSKLYKQQGERHKLEIIVNAQVRVH
                     EEWSSDNWNGGTYGHALYLTIPETIYVSCIDRRDELQNEIKSDLNKVHNVQNEFIEEV
                     FIEMEAREDDDWRRNSGVLRSGERTISDSAAERIWVSGTYRVFLSHKSEVKKETAEVK
                     TELAPFGICSFVAHEDVHPTEEWQEEIENALASMDAFVAFLTEDFHDSFWTDQEVGYA
                     VARGVPIIAVKFGIDPYGFIGRFQALSRGWDEAPLSIVKLLIKQPRMLDAYIAALSRC
                     SSFEDANVLANVLPEIGRLGIEQAGQLMSVFNANPDLRGSFGFRGRYPTQYGPGLAKY
                     LTRATGQQYVITTSGEMKRAKDITVGNSRK"
     gene            complement(2769..>5963)
                     /gene="carB"
                     /locus_tag="VFC15_04800"
     CDS             complement(2769..>5963)
                     /gene="carB"
                     /locus_tag="VFC15_04800"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZM09511.1"
                     /translation="GAQACKALKSEGYEVVLANSNPATIMTDPEMADRTYIEPLTRTY
                     LEEIIRIETEMLTAAGRPGIFALLPTVGGQTALNLAVELADGGVLEKYGVQLIGAQLG
                     AIKKAEDRLLFKDAMTAIGLNVPKSSLVNNLKDGIDFSGKIGFPVILRPSFTLGGTGG
                     GIAYNREELLELLARGLDLSPVHEVLLEESVLGWKEYELELMRDLADNVIVICSIENF
                     DPMGTHTGDSITVAPAQTLTDREYQAMRDAAIAVIREIGVETGGSNIQFAVDPHNGRM
                     VVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEITNDITRKTPACFEPTLDY
                     VVVKIPKWQFEKFPGADESLGPQMKSVGEVMAIGRTFKQALLKAVRSLETGRRATAND
                     IEPRILTKRLVTPHPERLAYIRYGFSQGMTVREIAQMTNIDPWFLHQLKEISDVQATL
                     GSGDIDAERLREIKRMGFSDARIADSLTLKEGRAAGEKVYQLRKQHNVQPVYKRVDTC
                     AAEFESYTPYLYSTYEEEDEATPTDRKKVIILGSGPNRIGQGIEFDYCCCHAAFALRD
                     DGFETIMINCNPETVSTDYDTSDRLYFEPLTYEDVMAVYEHEAAGGAPIGVIVQFGGQ
                     TPLNLALPLKAAGVPIIGTSPESIDLAEDRKRFGKLLEELDIPQPPGATAASVEEALA
                     NANRVGYPVLVRPSYVLGGRAMVIAYDDESVIHYMKEAVEYSQERPVLIDHFLESATE
                     IDVDALSDGEDVVIGGIMQHIEEAGIHSGDSSCVLPAVDIPERQLATIRDYTFRLARA
                     LKVIGLMNVQYAIQRDKVFVIEVNPRASRTVPYVSKATGVPLAKIAARLMTGRKLREF
                     LPENIERASDLDTGNFYFVKSPVFPWGKFPGVDTVLGPEMKSTGEVMGVADNFGEAFA
                     KAQTAAGQVLPTTGTVFISANDRDKPYMADLGKRFVELGFSLVATHGTARILEHAGLR
                     VERVYKVKEGRPNVVDLIKGERIQLIINTPHGVEPWFDEKAIRRAAVTARIPTITTLS
                     AARAACEGIAAMQRGEAKVYALQQLHAERIAAAR"
BASE COUNT         1177 a         1758 c         1701 g         1327 t
ORIGIN      
        1 ccttgattac gggacgctgc agattcccag cttgtgtgga catagttgtt ggcgcggccc
       61 cgggcagggt tcggttcgca ggaaccctgc aaccggagta catcagtgta ctgcctttag
      121 tgcttatcgg tcagtgcttg accaatttcg gcgagcattt tcaagtggat gcttcgccga
      181 ggcattccga agggcggcca cgcagaggga cggaaaattc accacggagg cacggagacc
      241 acggagaaaa ttctatggaa ccaaaactcc gtgcctctgt gtcggagcct gccctgagcc
      301 agcgacggtt tcgcgaaacc gtccgtgaag acagaagcta aacgagcgtg tatgttcgcc
      361 ttaaattcgc tttttttggc gtcgcatccg tcccggcgta ttccattgat cctcaatgac
      421 ttgctcatat agcttctgga gagcgaagcc gaagggtggt gaatggattt tcgacggagc
      481 gaggccgcgc cccgccacgg cgggtggccg agtcgaagga cccctatagc cgctcaaggc
      541 tatccttgga acgtagttcc tgttcagttc cgtatgtttt ccaacagctt ctccacttcg
      601 gttttcttgt cggccgtgag cggcagtagc ggcagacgcg ggacgccacc gaagcacccg
      661 ttgaaatcca tggcatactt cacgcccggg atgcttaagg cgccgactat gcgctcggcc
      721 gctttggcta tacgctcctg cttctcgcga gcgagagccg cgtcaccgtc tttccacgcc
      781 gcgtaaatct catagcaggc agtgggagcg cagtcggcga atgcgaggat cgcacccacg
      841 gctccggcat cgagcgaagg atgcaacttc tgggcggcgc ccaccagcac ctggaagccg
      901 acttccttct gtcgagtttt caggccgccg acaaccttca ccgacacgga ggacggtttc
      961 ggcgcggccg ttccggccag agcgcccaca gtcaccaagg ctcctccctc ggcgcttgcc
     1021 gaagctgcgg tggccgcatg cagcattcgt ccggtcacag cggcaaaggt ctccgtcacc
     1081 gtggcgctgc gcttgatgtg gcgggtagct tcgaccatct tttgcacctt ctcgaccgaa
     1141 cccgaagact ccttgatgcc gatcagattg gggtgatcgg caagttcaac cactacttca
     1201 gaaggaatgt cgtatccagt ggcctgcggg aagttataga tgatcgccgg caggggcgaa
     1261 cggtcggcca cgaagcgata gaaggccagc atgttggccg gcgtcatctg ccctttgtag
     1321 taatgcggcg tacgcaccat cgcgatatcg tagcccagct cagcggcata ttcggtcagg
     1381 cgcaaggttt cgatggccga ctcgattccc gttccggcga ccaacacttt ttcgttcgca
     1441 gcagattcgc gggcacactt caacacttcg cggcgctctt cgtcgctgag gagtatcgcc
     1501 tcgccagtag agccgagcac gacaatgcca accactggcg tgagcgagta gtgctcgacg
     1561 ttctgttcca gcttgcggaa gtagattttg ccgtcgggat aaaacggcgt ggtaatggga
     1621 ggaaagattc cgtgaagaaa cataatgcct ccagttttct gtcgcggctg tactcatcta
     1681 ttttctagaa ttgccaacgg taatatcttt cgcccttttc atttcaccgg atgttgtgat
     1741 cacgtactgc tgcccagtcg ctcgggttaa gtacttcgcc aaaccagggc cgtactgtgt
     1801 gggatatctg cctctaaacc cgaaactccc gcgcagatcg ggattcgcgt tgaagaccga
     1861 catgagctga ccggcctgtt caatacccag tctacctatc tctggcagaa cgttggccag
     1921 aacattggca tcttcgaagc tactgcagcg ggataatgcc gcgatatagg catccagcat
     1981 tcgcggttgt tttatgagta gtttgacaat tgacagaggg gcttcatccc agccccggga
     2041 gagtgcttga aatcttccga taaatccata cggatctatt ccgaacttta ctgcgattat
     2101 cggaactccc cgtgcgactg cgtaccccac ctcttgatcc gtccaaaagc tgtcatgaaa
     2161 gtcctcggtc aagaatgcga cgaaagcatc cattgacgct aatgcatttt cgatttcctc
     2221 ctgccactcc tccgtcgggt gtacatcctc gtgagcgacg aaggagcaga tcccgaatgg
     2281 cgcaagctcc gttttaactt ccgccgtttc tttcttcact tcggacttgt gacttagaaa
     2341 tactcgatac gtaccgctaa cccatatacg ttccgcagcg gagtccgaga tcgttcgctc
     2401 cccagatcga agcactccgg aatttcgccg ccaatcgtcg tcttctctcg cctccatttc
     2461 aatgaagact tcttcaataa attcgttctg aacgttatgt actttattga ggtcagactt
     2521 tatctcattc tgaagctcgt ctctccgatc gatgcagcta acatagatag tttcggggat
     2581 cgtcaaatag agcgcatggc cgtatgttcc tccgttccaa ttatcagaac tccactcctc
     2641 atgcacccga acttgagcgt tgacaataat ctcaagcttg tgacgttcgc cctgctgttt
     2701 ataaagcttt gacaaagcag acagataccg ctcaatcccg acaggtaact cgaatttgct
     2761 gtcccgattc atctcgccgc cgctatccgc tccgcatgca gttgttgcaa ggcgtacacc
     2821 ttggcctcgc cgcgttgcat tgcggcgatt ccctcgcatg cggcacgcgc ggccgacagc
     2881 gtcgtgatgg tcggaatgcg cgcggtcacg gcggcacggc gaatcgcttt ttcgtcgaac
     2941 cacggctcga cgccgtgcgg cgtattgatg atcaactgaa tgcgctcgcc cttgatcaga
     3001 tccaccacgt tgggccggcc ttccttcacc ttgtatacgc gctcgacgcg caagccggca
     3061 tgttccagta tgcgggccgt cccgtgagtg gcaacgaggc tgaaacccag ttcgacgaag
     3121 cgtttcccca agtccgccat gtatggcttg tcgcggtcgt tggcgctgat gaacaccgtc
     3181 cctgttgtgg gcagcacttg tccggcggcg gtttgcgctt tggcaaaggc ctcgccaaaa
     3241 ttgtcggcga cgcccatcac ctcgccggtg gacttcatct ccggtcccag cacggtatca
     3301 actcccggaa acttgcccca cggaaagacc ggcgacttca caaaatagaa attgccggta
     3361 tccagatcgc tggcgcgctc aatgttctcc ggcagaaatt cgcgcagttt acggccggtc
     3421 ataaggcgag cggcgatctt ggccagggga acgcccgtcg ctttcgacac gtagggcacg
     3481 gtacgcgagg cgcgcggatt cacctcgatg acaaacacct tatcgcgctg gatggcatac
     3541 tgcacgttca tcagaccgat tactttcagg gcccgcgcca agcggaaggt gtaatcgcgg
     3601 atcgtcgcca gttgccgctc gggaatatcg accgcgggaa gcacacacga cgagtcgccg
     3661 gagtgaatgc cggcctcttc gatgtgctgc atgatgccgc cgatgaccac gtcttcgcca
     3721 tcggagagcg catccacatc aatctcggtg gctgactcga ggaagtggtc gatcagcact
     3781 ggccgctcct gcgagtactc gaccgcctcc ttcatgtaat ggatgaccga ctcgtcgtcg
     3841 taggcgatga ccatggcgcg tccgccaagc acgtacgagg gccgcaccag taccggatag
     3901 ccgacgcgat tggcgttggc gagtgcctcc tcgacacttg ccgccgtggc tcccggaggc
     3961 tggggaatgt ccaactcctc cagcagcttt ccgaagcgct tgcggtcctc ggcaaggtcg
     4021 atgctctcgg gtgaggtgcc gatgataggc acacctgccg cctttagcgg cagcgcaaga
     4081 ttgagcgggg tctgtccgcc gaactgcacg atgacgccaa tcggggcgcc gccggcggct
     4141 tcgtgctcgt acaccgccat cacgtcttcg taggtgagcg gctcgaagta gagccggtcg
     4201 ctggtgtcgt agtcggtaga gacggtctcg ggattgcagt tgatcatgat cgtctcgaaa
     4261 ccgtcgtcgc gcagcgcgaa tgcagcgtgg cagcagcagt agtcgaactc aattccctgc
     4321 ccgatgcggt tgggcccact acccaggatg ataactttct tgcgatcggt cggggtggct
     4381 tcgtcctctt cctcgtacgt cgagtagagg tacggggtgt agctttcaaa ctccgcggcg
     4441 caggtgtcca cgcgcttgta caccggctgg acattatgct gcttgcgcaa ctgataaact
     4501 ttctcgcccg cagcccggcc ttccttcagc gttaggctgt ccgcgatgcg tgcatcggag
     4561 aatcccatgc gcttaatctc gcgcaaacgc tcggcatcga tatcgcccga gcccaacgtc
     4621 gcctgcacgt cggagatctc ttttaactgg tgcaggaacc agggatcgat gttggtcatc
     4681 tgcgcgatct cgcgcaccgt cattccctga gaaaacccat agcgaatgta agccagccgc
     4741 tcgggatgcg gcgtgaccag gcgcttggtc aggatgcgcg gctcgatatc gttggcggtg
     4801 gccctgcgtc ccgtttcgag cgagcgtacg gccttcagga gcgcttgctt gaaggtgcga
     4861 ccgatggcca taacttcgcc caccgacttc atctgtgggc cgagcgactc gtccgcaccg
     4921 ggaaacttct cgaactgcca cttgggaatc ttgaccacga cgtagtcgag cgtgggctcg
     4981 aagcaggccg gcgtcttgcg cgtgatgtcg ttggtgatct cgtcgagcgt gtaaccgact
     5041 gcgagcttgg cggcaatttt ggcgatggga aatccggtgg ccttcgacgc cagcgccgaa
     5101 gagcgcgaca cgcgcgggtt catctcgatg acgaccatgc gtccgttatg cggatcgacg
     5161 gcaaactgga tgttcgatcc cccggtttcg acgccgatct cgcgaatgac ggcgatggcc
     5221 gcgtcgcgca tggcctggta ttcgcggtcg gtgagcgttt gcgcaggagc gacagtgatg
     5281 gaatcgccgg tgtgggtgcc catgggatcg aagttctcga tggagcagat cacgatgacg
     5341 ttgtcggcga gatcgcgcat caactccagt tcatattcct tccagccgag tactgactcc
     5401 tcgagcagca cttcgtgaac cggagaaaga tcgagtccgc gcgcgagcag ttccagcagt
     5461 tcttcgcggt tgtaggcgat accgccgccc gtgccgccca gcgtgaacga aggacgcagg
     5521 ataaccggga aaccgatctt gccgctgaag tcgattccat ctttcagatt gttgaccagc
     5581 gatgatttcg gtacgttaag gccaatggct gtcatcgcat ctttgaacag caaacggtct
     5641 tcggccttct tgatcgcgcc aagctgcgcg ccgatgagct gcacgccgta cttttcgagt
     5701 acgccgccgt cagcgagttc gacagcgagg ttcagagcgg tttgtccacc aaccgtcggt
     5761 aagagcgcaa agataccggg cctgccagcg gcagtaagca tctcggtttc aattcgaatg
     5821 atctcttcca gataggtacg ggtgagtggc tcgatgtagg tacggtcagc catctccggg
     5881 tcagtcatga tggtggccgg attggagttg gccagcacca cttcgtaacc ttcagacttc
     5941 agggccttgc aggcctgcgc gcc
//
LOCUS       DASRKK010000225         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1091498, whole genome shotgun sequence.
ACCESSION   DASRKK010000225 DASRKK010000000
VERSION     DASRKK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091498"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            614..>2257
                     /locus_tag="VFC15_04805"
     CDS             614..>2257
                     /locus_tag="VFC15_04805"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZM09512.1"
                     /translation="MKTRVAATAVLALVLFTIVALVSCEKPKAPEQAQQTGQGAPAST
                     EAPPKPNPDRNAYFGEEHIHTSWSVDAWLMGNRLTGPDDALKYAQGQTIKHPLGYDIK
                     IDTPMDFMGVTDHSEYVGVTKEANTPGSALSKLPAAQPLILTDPNNQADIQKVFTYLV
                     NMLAGPPVKALMSPEVAGSIWKDNVKIADENNHPGKFTAFCSYEYTSAPDNRNLHRNI
                     FFRDCDKVPVMPYSALDSWHPEDLWKWMDTQRKAGNELLAISHNANLSDGWMYPTDVD
                     SFGRPIDAAWASARDRNERLVEIKQIKGQSETHPLLSPNDEFANYELFTGLLGAPPTV
                     GRVDHIQGSFARQALKDGITMQDVRGYNPYKLGMAGGSDSHNTGSPYRQDNFYGGHAQ
                     IDGTVDRRMAGVMAFGTIDVRLENPGGLTGVWAEENTRASIWDAMYRKETFGVSGPHI
                     KVRFFGGWDYNKDILNASDWVHQSYAKGVPMGADLPPLKGTAPTFVVWAVKDPTSGNL
                     DRIQVIKGWTKNGQSFEKIFDVAWSGDRKPNKWSGRVPAI"
BASE COUNT          484 a          693 c          633 g          447 t
ORIGIN      
        1 gcaccagtgg gaagtcggcc ttagcttgcc gaacgtgatc cttgtgacaa ggtgccactc
       61 ccggcagctc catacaagtt tctggtacca gtgatcgttc cgaaggcacg atcgaatgct
      121 tttataggac ttctggggaa cttcgcgcct cgccggccgc aatccgccag tgcattccag
      181 ttgcccctgt gttccccctg ttgcacaagc ctcctcgctc gcctagaatt tcctgtccaa
      241 gaaattctac ggcctcagtc cggtcatgtg cccgaaaata tctatcatcg cggcgttgtc
      301 ctgcgaggga tccgagtgga gtcggcaacg aaatacagaa cgattggatc ggcggtcctg
      361 atgtttgcgt tggccgcgat tctcgcaatt gtgtctgccg ttgcgcagga gaagaatccg
      421 gaccgcaacg catgcttcgg cgaaacgcac cttcacactt gctggccggt ggatgcctgg
      481 atgttcggca accgttttac aggtccggac gatgcgtaca agttctcaag tgtttgagtt
      541 tgtaatacaa cgcccgcgtg cgcttcacct gcgcaggatc gtggtcgtgc cattcagcga
      601 gacggaggca tttatgaaga caagagttgc ggcaacggcg gttttggcgt tggtcctttt
      661 caccatcgtc gcgctagttt cgtgtgagaa acccaaagca ccagaacagg cacagcaaac
      721 tggccagggt gctccagcct cgaccgaagc gcctccgaag cccaaccccg accgcaatgc
      781 ctatttcggc gaggaacaca tccacaccag ttggtcggtg gatgcctggc tcatgggcaa
      841 ccgcctcacc ggacctgatg acgccctcaa gtatgctcag ggccagacta tcaagcatcc
      901 cctgggttac gacatcaaga tcgacacgcc catggacttc atgggtgtga ccgaccactc
      961 cgagtacgtc ggcgttacca aggaagccaa cacgccgggt tcggcgctca gtaagctgcc
     1021 cgcagctcag cctcttattc tcacggaccc caataaccag gcggatattc agaaggtctt
     1081 cacgtacctc gtgaatatgc tcgccggacc tccggtgaaa gctctgatga gccccgaggt
     1141 ggccggctcg atctggaagg acaacgtcaa gatcgccgac gagaacaatc atccgggcaa
     1201 gttcactgct ttctgctcgt acgagtacac ctccgcccct gacaaccgga acctgcaccg
     1261 caatatcttc ttccgcgatt gtgacaaggt gccggtgatg ccctacagcg ctctggactc
     1321 ctggcatccc gaagacttgt ggaagtggat ggacacacag cgcaaggcag gcaacgagtt
     1381 gctggccatt tcgcataacg ccaacttgag cgacggctgg atgtatccca ccgacgttga
     1441 cagtttcggc cgccccatcg acgcagcctg ggcatccgcc cgcgaccgca acgaacgcct
     1501 ggtcgagatc aagcagatca agggacagtc ggagacccat cccttgctct cgcccaatga
     1561 cgagttcgcc aactacgagc tcttcaccgg cctgctcggt gcgccaccga ccgttggccg
     1621 ggtcgatcac attcaaggaa gctttgcccg ccaggcactg aaagacggca tcaccatgca
     1681 ggacgtgcgc ggctacaatc cttacaaatt gggcatggct ggtggctccg actcccacaa
     1741 caccggcagt ccgtaccgcc aggacaattt ctacggcggt cacgctcaga tcgacggaac
     1801 cgttgaccgg cgcatggccg gggtcatggc gtttggcacg attgacgttc gcctggagaa
     1861 tccgggcgga ctcacaggcg tgtgggccga ggaaaacact cgtgcgtcca tttgggacgc
     1921 gatgtatcgc aaggaaacct tcggcgtcag cggcccgcac atcaaggttc gcttcttcgg
     1981 cggttgggac tacaacaaag acatcctgaa cgccagtgac tgggtgcatc agtcgtatgc
     2041 gaagggcgtc cccatgggcg ccgacctgcc tcctctgaag ggcacggctc cgacatttgt
     2101 tgtctgggcg gtgaaagacc cgacctccgg caatctcgac cgcatccagg tcatcaaagg
     2161 ctggaccaag aacggccaga gcttcgagaa gattttcgac gtagcctggt ccggcgaccg
     2221 caagcccaac aagtggtcag gcagggttcc ggcgatc
//
LOCUS       DASRKK010000226         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_895859, whole genome shotgun sequence.
ACCESSION   DASRKK010000226 DASRKK010000000
VERSION     DASRKK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895859"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /gene="glmS"
                     /locus_tag="VFC15_04810"
     CDS             <1..1243
                     /gene="glmS"
                     /locus_tag="VFC15_04810"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZM09513.1"
                     /translation="FFLADGDLAVITPSGVQLTDFDGQPITREVQHITWDPIMAEKGG
                     FKHFMLKEIYEQPRAVRDTTLGRVSLDSGKVFLDEMDITEEDFRKLEKVHIAACGTSW
                     HAGLAGKFMIERLAKLPVEVDYASEFRYRDPLTGPGDLTILITQSGETADTIAAMRES
                     RAKGSKTLGICNVVGSMIAREANGTVYTHAGPEIGVASTKAFTAQLTALFLFAIHLAQ
                     VRGTLDLDHSKQLLNDLTLMPGKLEALLTHDEETEDLAKQYHRAHDFLFLGRGIHYPI
                     ALEGALKLKEISYIHAEGYPAGEMKHGPNALIDEDLPVVILATRDKTDPGSVVRYEKT
                     LSNLKEVKARSGTVIAIATEGDEDIREAADHVLYVPDAPELLAPILEVVPCQLLAYHI
                     AVRRGCDVDQPRNLAKSVTVE"
     gene            complement(1360..1752)
                     /locus_tag="VFC15_04815"
     CDS             complement(1360..1752)
                     /locus_tag="VFC15_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HZM09514.1"
                     /translation="MKRLRWLTAKAVLAIHEELIVRYGGVSGLRDDGLLESAVARPQN
                     LVAYGQKISVASLSAAYAWGLLRNHPFVDGNKRTALAAMVVFLELNGWELGCSEAEET
                     AMVLRAAAGEITERAWTEWVRRSTKKKS"
     gene            complement(1749..1970)
                     /locus_tag="VFC15_04820"
     CDS             complement(1749..1970)
                     /locus_tag="VFC15_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09515.1"
                     /translation="MQTKLRKIGNGYGVLLPKQVIERLRVSEGGVLNLTETSSGIELS
                     PFDANFSEQVEAFRRTESRHRNSYRELAK"
     gene            2082..2834
                     /locus_tag="VFC15_04825"
     CDS             2082..2834
                     /locus_tag="VFC15_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM09516.1"
                     /translation="METQVQERTTVERQMFTARLSRSIILSPQTKHLEFTVEGLEEFY
                     FVPGQFVSIKQPRPDGKTHTRAYSIASPPRLNATFDLCLNRVEEGFLSNWLCDLPEGA
                     TVQFHGPHGLFTLREPRKDAIFIATGTGIAPIRGMVHWLFEKPERNRGHEYWLVFGTR
                     YEHSLYYRDEFERIARENPNFHYMPTLSRCGELWTGCRGYVQDHVREIVAGRTDMQAY
                     ICGLHQMVDASRKLLKEELGWDRKQIVFERYD"
     gene            3051..3581
                     /locus_tag="VFC15_04830"
     CDS             3051..3581
                     /locus_tag="VFC15_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09517.1"
                     /translation="MGRLAIIAACILLISSGSLAQDWKQVRKADEAKWAKATGLDPRT
                     IHKLWRQASQVANEKDDDSRIANLEVESLSDRHQVLLVTYTGEKNCLVLTVFRQYSAT
                     AFSKLWSVEQDPDGQGFCDSPFGNASADIVNHAIEVRVPQPPDASNGYANYKVYGYEW
                     NGITYRFTSKRAEQGR"
     gene            complement(3878..4333)
                     /locus_tag="VFC15_04835"
     CDS             complement(3878..4333)
                     /locus_tag="VFC15_04835"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZM09518.1"
                     /translation="MIELVLPNDTNPLGALLGGRLMHWIDLAGALAAHRHSHSYAVTA
                     SMDHIDFLVPVHVGDMVILQSSVNRAFNTSMEVGVRVLVENYISASRQKVATAYLTFV
                     AVDRYGRRLKVPPVIPETEEEKRRYEGALRRREASKAERERRNAARGEE"
     gene            complement(4415..4894)
                     /locus_tag="VFC15_04840"
     CDS             complement(4415..4894)
                     /locus_tag="VFC15_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09519.1"
                     /translation="MACPFFMPEQRFDSDWPFPQRLPLGAGWTGTCTAPGHEGARPTD
                     EELKSGCNLGYAKTCSRLPAERHADAVRFVLGEERDGILRVLFVCERDYLPAGHGELL
                     YKTANASWLHSHPDPRVQRMAECYVQTQRTRRANLLPADQIATAAPAAAQQSAATAK"
     gene            5060..5383
                     /locus_tag="VFC15_04845"
     CDS             5060..5383
                     /locus_tag="VFC15_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09520.1"
                     /translation="MPDETRVAALKEILDANPDDAFARYALGLEYSGSGETDAALAEF
                     QRLLASHPDYTNGYFMAAQTLARAERKDEAQSLLQQGIECARRTRNQHALAEMEAMLD
                     ELERG"
BASE COUNT         1181 a         1772 c         1625 g         1174 t
ORIGIN      
        1 gttctttctc gcggatggcg atcttgccgt catcacgccc tcgggcgtgc agctcaccga
       61 cttcgacggc cagcccatca cccgcgaggt gcagcacatc acctgggacc cgatcatggc
      121 ggagaagggc ggcttcaagc atttcatgct gaaggagatc tacgagcagc cgcgtgcggt
      181 acgcgatacc acgctgggcc gcgtctcgct ggactcgggc aaggtgttcc tcgacgagat
      241 ggacatcacc gaggaagatt tccgcaagct ggagaaggtg catatcgcgg cttgcggcac
      301 cagttggcac gccggactgg cgggcaagtt catgatcgag cgactcgcta agcttccggt
      361 ggaagtggat tacgccagcg agtttcgcta tcgcgatccg ttgaccgggc ccggcgatct
      421 gacgatcttg attacgcagt caggcgaaac cgccgacacg attgcggcca tgcgcgaatc
      481 gcgcgccaaa ggctcgaaga cgctcggcat ttgcaacgta gtgggctcca tgatcgcgcg
      541 cgaagccaac ggcaccgtct acacccacgc cggaccggag attggcgtgg cgtcgaccaa
      601 agctttcacg gcgcagctca ccgcattgtt cctgttcgcg atccacctcg cccaggtgcg
      661 tggaacgctc gatctcgacc actccaagca gctcctcaac gatctcacgc tcatgccagg
      721 caagctggaa gctttgctta cccacgacga agaaaccgag gacctcgcga agcagtatca
      781 tcgcgcgcac gacttcctct ttctgggacg tggcattcac tacccgatcg cgctggaagg
      841 cgccttgaag ttgaaggaaa tctcctacat tcacgccgag ggctatcccg caggcgagat
      901 gaaacatgga ccgaacgcgt tgatagacga agatcttccc gtggtgattc tcgccactcg
      961 cgacaagacc gatcccggct cggtggtgcg ctacgagaag acgctgtcga acctgaagga
     1021 agtgaaggcg cgttccggca ccgtgattgc catcgccacc gagggagacg aagacattcg
     1081 cgaagccgcc gaccatgtct tgtacgttcc cgatgcgccg gaactgctcg caccgattct
     1141 ggaggtggtt ccctgccagt tgctggcgta tcacattgcc gtccgccgcg gctgcgacgt
     1201 ggaccaaccg cgtaatttgg ccaagtcggt gacggtggag tagggcaggc gctattcgcc
     1261 tttcgctatt cgcttttggc ttcgtcgttt ctcgtcgcac cactcgcgtg agtaacccgc
     1321 ccgccctcgc cgcgcaacga cagggcgaga tttgctatct caagattttt tctttgtgct
     1381 tcgccgcacc cactcggtcc acgcgcgctc cgtgatctcg cccgcagcgg ctctgagaac
     1441 catcgccgtt tcctcggctt cgctgcaccc gagttcccat ccgtttagct caagaaaaac
     1501 caccatcgcc gccagcgcgg tgcgcttgtt gccatcgacg aatggatgat tccgcagcag
     1561 cccccaggcg tatgcggccg acagactggc caccgatatt ttttgtccat atgcaaccag
     1621 gttctgaggc cttgcgacgg cggattcaag tagcccgtcg tcacgcaggc cggagactcc
     1681 gccgtaacgc acaatcagtt cttcatggat ggccagcacg gctttcgcgg tcagccatcg
     1741 caggcgcttc acttcgccag ttctcgatag ctgttacggt gccgcgactc ggtccggcgg
     1801 aaagcttcga cttgctcgga gaaattggca tcaaagggtg acaactcaat cccgctactt
     1861 gtctcggtta gattcaatac accaccctcg ctcacgcgaa ggcgttcgat gacctgcttg
     1921 ggcagcagga ctccatatcc atttccgatt ttgcgaagct tagtttgcat aaaggcatta
     1981 taacattagt tataatgcct gccaggccgt ttcatcttgg cggaatacgc gctcccccgc
     2041 gacgattttc gcctgacggg catctacata agcagacctt tatggagacc caagtccagg
     2101 aacgcacgac cgtcgagcgc cagatgttca cggctcgctt gagccggtcc atcattctgt
     2161 caccgcagac caagcacctg gagttcacgg ttgaaggatt ggaagaattt tactttgttc
     2221 ccgggcagtt tgtttccatc aagcagcccc ggcccgatgg caagacgcac acccgcgcat
     2281 actccatcgc ttcgccgccg cgcttgaatg ccaccttcga tctgtgcctc aaccgcgtgg
     2341 aggagggctt tctttcaaac tggctttgcg atctgccgga aggtgcgacc gtgcagttcc
     2401 atggtccgca cggccttttc accttgcgcg agcctcgcaa ggacgccatc ttcatcgcca
     2461 ccggaaccgg catcgcgccc atccgcggca tggtccattg gctcttcgag aagccggaac
     2521 gcaatcgcgg ccacgaatac tggctagtct tcggcacgcg ctacgagcac agcctctact
     2581 accgcgacga gttcgagcgc atcgcacggg agaacccgaa cttccactac atgccgacgc
     2641 tcagccgctg cggcgagctt tggacgggct gtcgcggcta cgtgcaggac cacgtccgcg
     2701 agatcgtcgc cggccgcacc gacatgcagg cgtacatttg cggcctgcat cagatggtgg
     2761 acgcgagtcg caagctgctc aaagaggagt tgggctggga ccgcaagcag atcgtgttcg
     2821 agcgctacga ctaaacgaga actcatcgca taagaattgt cattacgagc gaagcgagga
     2881 atctgctttt tgctagcgct ggaaagattg ttcggaggaa acagcagatc cctcacggcc
     2941 tgaaggccgt tcgtgataac aactcaaaat caccggcaca gccactctgg aatccctcta
     3001 aactaaaatc ttgccctcac aacctggctg cgccatgatg aagatgagct atgggccgtc
     3061 ttgccatcat cgctgcttgc attcttctga tttcgtcggg atcgctcgca caggactgga
     3121 aacaggtgcg caaggccgac gaggcaaagt gggcgaaagc caccgggctc gatccccgaa
     3181 ccatacataa gctctggcgc caggcctcgc aggtggcaaa tgagaaagac gacgactcgc
     3241 gtattgccaa cctggaagtc gagagtctct ctgaccgcca tcaggttctg ctcgtcacct
     3301 acacgggaga aaagaactgc cttgttctca ccgtcttccg gcaatactcg gcgacagcat
     3361 tttccaagtt gtggtcagtc gagcaagatc ccgacggtca aggattctgc gatagtccgt
     3421 tcggcaacgc cagtgccgat atcgtgaacc acgccatcga ggtgcgcgtg ccgcagcccc
     3481 cggatgcctc caacggctac gccaattaca aggtttacgg ttacgaatgg aacggcatca
     3541 cctaccgctt cacgagcaag cgagcagagc agggccggta agccagaagc cagggtttgg
     3601 cctcaggcca gttgccgcaa tgttgtgccg atccgccgag gtgcacttcg cagttggtta
     3661 ggccagtttc agagttggtg tagaccgggt agagccagcc ttcaggctgg cgtccaagat
     3721 cacccacacc ataaggcttg agccgctgcg gtcattttca atgaccgcag cggctcaagc
     3781 gattcgttta tccgcggcga acgcggcgct gaagcgccgc tctacccgta ctcgccggaa
     3841 tacaccaatt ctgaaaccgt tctacctcaa cggattttta ctcttcccct ctcgccgcgt
     3901 ttctgcgctc gcgctccgct ttcgaagctt cccggcggcg cagcgcacct tcgtagcgcc
     3961 gcttttcctc ctcggtttca ggaatcacgg gcggtacctt cagccttctg ccgtaccggt
     4021 ccacggcaac gaacgtcaga taagcggtcg cgactttctg gcgcgaagct gagatgtagt
     4081 tctccaccag aactcggacg ccaacttcca tcgaagtatt gaacgcgcga ttgaccgacg
     4141 actgcaagat taccatgtcg ccaacgtgca ccgggacgag gaaatcaatg tggtccatcg
     4201 aggcggtaac ggcgtaagag tgcgagtggc gatgcgccgc cagggcaccg gcaagatcga
     4261 tccagtgcat taaacggccg cccaacagcg cgcccagcgg gttggtgtca ttgggcaaca
     4321 cgagctcgat catttccgat ttcgagtcgc tcaccggtct cggtcgcaga tttccttcgt
     4381 tcggttccat agcgttcctt gtgtagaact ggatttactt cgctgtcgcc gcgctttgct
     4441 gcgccgcagc cggtgcagcc gttgcgatct ggtcagcggg aagcaggttc gcgcgccttg
     4501 tacgctgcgt ctgtacgtaa cactcggcca tccgttgcac gcgcgggtcg ggatgcgaat
     4561 ggagccacga tgcgttcgcg gtcttataca gcagctcccc gtgacccgcg ggcaggtaat
     4621 cgcgctcgca gacaaagagc acgcgcagaa tgccatcgcg ttcctcgccc aacacaaagc
     4681 ggactgcgtc ggcatggcgt tccgcgggca gccggctgca ggtcttcgca tagcccaagt
     4741 tgcagcccga cttcagttct tcgtcagtag gacgcgcgcc ctcgtgaccg ggcgcggtgc
     4801 acgttcccgt ccatcccgcg cccaagggaa gccgctgcgg aaacggccag tccgaatcaa
     4861 atcgctgttc tggcatgaag aagggacagg ccacaaaatc ctcgtccgcc gcagcgggct
     4921 gtgaaggtta aacgctattg tactggcagt gcggaaccgc ttccaactaa tgcaccgact
     4981 cgtcgattgg aatccgtaat cgagtttgag cgtttgatgg ctgctctagt aacatgcgtt
     5041 cgagactgga gatcgcataa tgcccgacga aacccgcgtg gcggcgctga aagagatact
     5101 ggacgcaaac cccgacgacg cttttgcgcg ctacgcgctt gggttggagt actcgggctc
     5161 gggcgagacc gacgctgcac tcgcggagtt ccaacgctta ctcgcgagcc atcccgacta
     5221 caccaacgga tacttcatgg cagcgcagac cctcgcgcgc gcggagcgca aagacgaagc
     5281 gcagtcgctg ttgcagcaag gcatcgaatg cgcgcgccgt acacggaatc agcacgccct
     5341 cgcggagatg gaagccatgc tcgacgaact ggagcgcggg tagtaatgga gaggagcgag
     5401 ccttacagaa gagctggctt ccagggagtg tgtgagaatt gtgccgtccc tgaagggact
     5461 ccttaatttt ctccactcta cccagcactc ccgccttcgg ctgcgtgctg ggctatagca
     5521 gtttcacatc tggtgtatag gcgatgggtg gagcacgcct tcaggcgtgc ataactgccg
     5581 tcaattcttg gatcgcgctt tagcgcctga agtaatgttt ccgtcacctc agtggctaaa
     5641 gccgtctcat tgccgaatcc taactgcacc gctgaagcgg tgctccaccc tatacaccaa
     5701 gtgtgaaaat gctctaagct ctttcgccct tgcgggctgg attttcagct ag
//
LOCUS       DASRKK010000227         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_16726, whole genome shotgun sequence.
ACCESSION   DASRKK010000227 DASRKK010000000
VERSION     DASRKK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16726"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..718
                     /locus_tag="VFC15_04850"
     CDS             314..718
                     /locus_tag="VFC15_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09521.1"
                     /translation="MRVTETVYKGKLRPWAKTESSEDYVPLPKRKAAELIDWRTLSTA
                     QGEQGFIFPNSQGGFVDYENFEARVLEPIRLELRLTKLNFQILRRTYATRAGGERKGT
                     LKDVQKQLRHSRPDTTLENYVQGDPGVGVPDG"
     gene            complement(874..950)
                     /locus_tag="VFC15_04855"
     tRNA            complement(874..950)
                     /locus_tag="VFC15_04855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Arg,seq:ccg)
     gene            1322..2290
                     /locus_tag="VFC15_04860"
     CDS             1322..2290
                     /locus_tag="VFC15_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09522.1"
                     /translation="MLLLASAQAQLRGGAGGSLAGSVHVHIVFDNERSAGPNLSVRLM
                     EGSSNTPIANTYTDSNGQAYFSGVRVGDYHVIVSGDGIQTTESRTFEVDERKVTQAQY
                     ITVHRTDDSSPTPAGSKSSMISAGELNVPAKARRELDKANEAMARHDWKKTLERLNKA
                     VAIDPQYAAAYNNLGALYARMNDSVHEREALEKAISLDDHLSAAYVNLAKLCLREKNF
                     PKAETLMGKAVSVDPNNADSLMLLADAQYMDHHYEAAIASARQAHASSNTHPAFVHYI
                     AARAYDMENRREEALAELQIFLSEEPTGPRADHVRAAIQSQPRPAQ"
     gene            complement(2748..2993)
                     /locus_tag="VFC15_04865"
     CDS             complement(2748..2993)
                     /locus_tag="VFC15_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09523.1"
                     /translation="MAEPSYEELKARVAELEKTQGRKRGSLEFKVSEKGGMSVYGLGR
                     FPVTLYYEQWIRLLDQTDQIREFLEANKAGMKLKNTE"
BASE COUNT          715 a          884 c          851 g          597 t
ORIGIN      
        1 tgcaagtggt gtcgagctac agcggccttg cgcacgacga cgccggcgac ttcaagaagc
       61 gggtggaaga atcgctgacc aaggtgaaca cgaagccagc cgacgccgcg aaaccgggcg
      121 acgcgaccaa gtgattttgg taggcccgtc cacgaatccg gcaagagcgg agttttgaaa
      181 gggcacagct tctcacttcg gcgtatttcc acgtggtgtt ctcaaccaaa gagcccggtc
      241 aattttccgc ctcgacccgg ttttatgacc gggctcggtg tctcgaaggc gtgggtgcga
      301 gaccacgcca cgcgtgcgcg taaccgaaac ggtttacaag ggcaaactgc ggccctgggc
      361 gaagactgag agcagcgagg attacgtccc gctcccgaag cgaaaagctg ccgaactcat
      421 cgattggcgc accctctcca ccgcacaagg cgagcagggt ttcattttcc cgaacagcca
      481 aggcggtttc gtggactacg aaaacttcga ggcgcgcgtc ctagagccta tccgcctcga
      541 actaagactg acaaagctga atttccaaat cctgcgaagg acctacgcaa cacgcgcggg
      601 cggtgagcgc aagggaacgc tcaaggacgt gcagaagcaa ctgcgccaca gccgaccgga
      661 caccacgctg gaaaactacg tacaaggaga tcccggagtc ggtgtaccgg atggttgatg
      721 ccatgtacga cgagatcaca cctgaagtga agctcttggg gggcaaacaa cgttctgggg
      781 agagttgtgc ccacccgaga tatccagtga gccagccaat tgaacacttt ctgtacacgg
      841 cgactgaggg caatcgccta agtgtttgaa atctggtgcg cccggagaga ttcgaactcc
      901 cgacctattg ctccggaggc aatcgctcta tccagctgag ctacgggcgc gctccttgaa
      961 attatacacg cgccatgccg atggccgcgg tgggcttttt cgcggcatta ggaccactgc
     1021 ctctggcgaa cctggcgatg gactgcggca cagcaccgtg actgtcaacc ggagaatgtt
     1081 cacaaattgt ctacaaccga ccaaacacca gcgggtgtag aatcgcgcca accaaaggat
     1141 gtcggtcgtg gggtacagcc gctgtgggaa cgaatgccct acgatcctgg ttctgcatcc
     1201 gggccgctcg cgaagagttc gcgcggcgag ttcgacccga cgctcttgag attgccggtc
     1261 tccgcgcgat ctcagggagg cagttatgcg gtatcaacgc tggatcgctc ctcttttcct
     1321 tatgcttctt ctcgcctctg cgcaggccca gctaagaggg ggtgcaggcg ggagcttggc
     1381 gggaagcgtg cacgtgcaca ttgttttcga caatgagcgt tccgcaggcc ccaatttatc
     1441 tgtacggttg atggagggtt ccagtaatac tccgatcgca aatacctaca cggattctaa
     1501 tggccaggcg tacttcagcg gagttcgtgt gggcgattac catgtcatcg tgagtgggga
     1561 tgggatccaa accaccgaga gccgaacatt cgaagtggac gaacgtaagg tcactcaggc
     1621 gcaatatatc accgtgcacc gcaccgacga ttccagcccc accccggccg gatcgaagtc
     1681 ttcaatgatt tcagccggcg aactgaacgt tccggcgaag gcgcggaggg aacttgacaa
     1741 ggccaatgaa gctatggccc gacacgattg gaaaaaaacg ctggagcggt tgaacaaggc
     1801 cgtcgccatc gatccccaat atgccgcggc ctacaacaat cttggcgcgc tttacgcccg
     1861 gatgaacgat tctgtccacg agcgggaagc tctggaaaag gccatcagcc tggatgatca
     1921 tctctccgcc gcatacgtaa acctcgccaa actgtgcctg cgagagaaga atttccccaa
     1981 agccgaaaca ttgatgggga aggcggtgag tgtggaccct aacaatgccg atagcctgat
     2041 gctgttagca gatgcgcagt atatggacca ccactacgag gctgccatcg ccagtgctcg
     2101 gcaagcccat gcttcgtcga atacgcaccc ggcattcgtg cactacattg cggcccgcgc
     2161 ctacgacatg gaaaaccggc gggaggaggc actcgccgaa ctacagatat tcctgagcga
     2221 ggagcctacg ggtccgcgcg ccgatcatgt gcgcgccgcc atccaatcgc agccgcggcc
     2281 tgcgcaatag gtcagcagcg ccggtaacga cttatacggg tagagctgga tttcacaggc
     2341 gactgaaaaa gtcggaaaag cagaactctc gcgggctgaa gcccgctcga gatgaaaaaa
     2401 taaagcttaa cggcgcacct aaaggtgcgc cccttcaaaa caacggctaa ggccaatttt
     2461 tttaacagcc tgtgaacccg actcaagctg atagacgatg cagttagacg cgggtctgaa
     2521 gagggtgtgt gaaaactgtg tcgtccctaa agggactcgt taatttttcc cactttaccc
     2581 agggcttacg ccctgggcta aaacaaattt cgcccctgcg gggctagagt ttgcgcaatc
     2641 gttccaccgt gccaactcag agcgagttct cacacacgct ctgaagcgcg ctccacccca
     2701 agctacagcc gttttggaac cggtctagaa gcgatcaacg aaaaacatca ctccgtgttc
     2761 ttcagtttca tgccggcctt gttcgcctcc agaaactcgc ggatctgatc ggtctggtcg
     2821 agcagccgga tccattgctc gtaatagagg gtgaccggga agcgtcccag gccatacacg
     2881 ctcatcccgc ccttttcgct gactttaaac tcgagactgc ctcgcttacg cccctgagtc
     2941 ttttccaatt ccgcgactct ggctttcagt tcttcgtaac ttggttcggc catattgatt
     3001 tcctctcgaa ataactaagc gcgaatcact gccgccccac atggccg
//
LOCUS       DASRKK010000228         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_16806, whole genome shotgun sequence.
ACCESSION   DASRKK010000228 DASRKK010000000
VERSION     DASRKK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16806"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(789..>2264)
                     /locus_tag="VFC15_04870"
     CDS             complement(789..>2264)
                     /locus_tag="VFC15_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09524.1"
                     /translation="QLQGDPAACVFANQTKLLLPRFLAKSVVVGDEITFPIPAPDAVG
                     PEIVVIKAAASGSSRYIYQAPIAYVSQPKQDKRNQYFVSAEVHRGSLGIGTIFLPCEI
                     LREYFYRLPRAADSVDHPTLYEILRIPASASPSELRVAFKLRDLELRTEGVGHRNRVT
                     LERAFNIVGQPELRACYDALLADPEAPAVFPYGGFGSLIVAGEPSRDGKTFFARRILA
                     FSPDLRRRRFHVPLRKCDFYDDRALCRDMRRKLEFWLDPAALHTLWDRTWNQWKHLLG
                     AKIEVEATFVQRGKYRKRQEQWDLVTWETALPSRLEVKLPPDFQQQMESAKTAHHRFG
                     QFSRALEQIRLCLEHRAVEREELQRMCAELRIPGDFDVAQISWRPDYDPFFYRQLSRR
                     ARRIYLFRDEYIFDVEKSVVVETPQLGHATYVFAKPRSLDSFLALYTKITKDDIRRNR
                     NNAAERLGFLGRVIHGTNPRAWLKEVRQRAGEGIDFATSEA"
BASE COUNT          493 a          635 c          647 g          489 t
ORIGIN      
        1 cgttctaaac ttccatgttg gtaacgtgtc cgtttaaaca aagctcctcc tttgttcaaa
       61 cggtgtcggc ggacattgtc agaatagttg ggatctgaga aatggtcgag aagttttgtt
      121 tgacccagaa cgacattcag cccaccattt tttcaagcca gtgattcagc gtagaggcac
      181 gaaaacgcca tcgccctccg acctgaagcc cggggatcaa tccttgacgt gcctttgtcc
      241 gcagcgtcct cggatgcatc ctcaggagaa tggcggcttc atcggtatcc agtaggggtt
      301 caaagtccac atgagtctcg actagctgtg gcccgttacc attcttacgt gttgatccac
      361 ttctttgact cgcattttcc agcatcgaat taccacctcc tcattaaaat ctcgcgtccc
      421 cgtaagacct ttccaagatt cgctctgaag ctgacgctta acgacgcacg gcgcgagaaa
      481 cggcatctcc agcactcttg ttagctttaa atcgagtggc agtcaaacag gctgatcagg
      541 cgagaaccgc caagcactgt gggctccgta tggacttgct tgtaatctgg cagctctggc
      601 aggagtttcg tgctaaaacc gctctcaatc caaggcggcg gtactccgga tgcgaaagct
      661 cgcggctcgt cctggaatca cggtcgtcaa tagatttttt tcaccagccg cactttgtct
      721 agtaaaagca ccgcgcaagc cggaagcgcg gccaatgcaa atgccgagaa cggcgtatgc
      781 ggatcggttc acgcttcact ggttgcaaag tcgattccct ccccggcgcg ctgtctgacc
      841 tccttgagcc atgctcgtgg atttgtgcca tgaatcaccc gccctagaaa gcccagccgt
      901 tcggctgcat tattgcggtt ccgacggatg tcgtccttcg tgatcttggt gtatagagcg
      961 aggaagctat cgaggctccg aggcttggcg aagacgtaag tcgcgtggcc aagctgagga
     1021 gtttcgacga cgacgctttt ctcaacgtcg aaaatgtatt catcccgaaa cagatagatc
     1081 cggcgcgcgc ggcgtgagag ttggcgatag aagaatggat cgtaatcagg gcgccagctg
     1141 atctgggcga catcgaagtc gccgggaata cgcaactcgg cacacattcg ctgcagctcc
     1201 tcccgttcga ccgccctgtg ctccaagcac agtcggatct gctccagagc ccgcgagaat
     1261 tgaccgaagc gatggtgggc agttttggca gattccattt gctgctggaa atcgggcggc
     1321 agtttgacct ccaaccggct cggcaacgca gtctcccagg tcacgagatc ccactgctcc
     1381 tgacgcttgc ggtacttgcc gcgctgtacg aacgtagcct cgacctcgat cttggctccc
     1441 agcagatgct tccactgatt ccaggtgcgg tcccagagtg tatggagggc ggctgggtcg
     1501 agccaaaact ccaacttgcg acgcatgtcg cggcacagtg cccggtcgtc gtagaaatcg
     1561 cattttcgca atggcacatg gaaccgccgg cgtcggagat cgggtgaaaa cgcgaggatg
     1621 cggcgggcaa agaatgtctt tccgtcgcgg gaaggttcgc cggcaacgat gagagagcca
     1681 aatccaccat aaggaaacac agcaggggct tcgggatcgg ctagcagcgc gtcatagcaa
     1741 gcgcgcagtt ccggctggcc aacgatgttg aaggcccgct ccagcgtcac tcggtttctg
     1801 tgccccacgc cctccgttct tagctccaga tcacgcagct tgaaagccac gcgaagctca
     1861 gagggcgaag cgctggccgg tatacgcaag atctcgtaca gggtgggatg atcgacgctg
     1921 tcggccgcac gcggcaggcg gtagaagtac tcgcgcagga tctcgcaagg caggaaaatc
     1981 gtgccgatgc caaggcttcc ccggtgaacc tcggccgaga cgaagtactg atttcgtttg
     2041 tcctgcttgg gctggctgac gtatgcgatt ggcgcttggt agatatagcg gctcgatccg
     2101 gaggcggcag ccttgatgac gacgatttcc gggccgactg catctggtgc tggaatcgga
     2161 aatgtgattt cgtcgccgac aacgacagac ttcgcaagaa aacggggcaa cagaagctta
     2221 gtttgattgg cgaagacaca ggcagccggg tccccctgca gttg
//
LOCUS       DASRKK010000229        11283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_407811, whole genome shotgun sequence.
ACCESSION   DASRKK010000229 DASRKK010000000
VERSION     DASRKK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407811"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFC15_04875"
     CDS             complement(<1..578)
                     /locus_tag="VFC15_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09525.1"
                     /translation="MNLESDKRLNIQLRLDFSSTPTGEARQAGREDIESLSVVLEPER
                     PANASRIKEEICERANLKEALRQVRSNKGSAGVDGMTVDKLGDYLKQHWPVIREQLLS
                     GIYEPKPVRRVEIPKPDGGGVRKLGIPTVLDRFVQQSLNIQGCEPLTMEVVLPELAAS
                     FGPSSADQVFAVFIIAHIELPRCELKPIGRVRT"
     gene            complement(1284..2441)
                     /locus_tag="VFC15_04880"
     CDS             complement(1284..2441)
                     /locus_tag="VFC15_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZM09526.1"
                     /translation="MNQADSKKTVLIVDDAPANIQVVNSILKDIYKIRIATNGAKALE
                     LAKATPPPDLILLDVIMPEMDGYEVCAQLKADPDTRDIPVIFLTGQTEVEDETRGFDA
                     GAVDYVHKPFSPAVVKARVQTHLMLRGIREQLARQLLAIQKEMETARQIQLSILPSQI
                     PKIEGLDIAARYVPMTSVAGDFYDFIVVDEKHIGILVADVSGHGMPAALIASMLKIAL
                     SAQVAQAADPAQVLLGLNQALCGKFQHHYVTAAYLFVDLQKRTLTYAGAGHPPLLLWG
                     GSSDGGRAVEENGLFLGAFEFATYSSVELPLQPGHWGLLYTDGISETTNPLGIEFGTD
                     RFREFLAKEESTSADHFADRLLEELSLWSARGPNEELDDDITLVALHVKGD"
     gene            complement(2438..6025)
                     /locus_tag="VFC15_04885"
     CDS             complement(2438..6025)
                     /locus_tag="VFC15_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM09527.1"
                     /translation="MGLLANFKIRTKVLVAMLPLALMAIGAGVYSSIESQKIDTWYSN
                     LINNYVKTFQSVTAARGNTMRFRLLLYQLVAEDNPDQRQENDGELDKVQADYRALLAD
                     ALRQNPGRASEIKAAETVFDRAASSARPVRAAALAGNREKAMNIMRSGVDADLQQARQ
                     SMTDLVDEMQKSANQESDELTSRTHRAILITWIVIVLGLVVSFVVALSIVQVEVVKVV
                     LSFRSRILDVAEGRLDLPVANLNRSNEIGEMSRALQTLQMAAREREILGWVKAEVAAT
                     TERLQSAENFPAFATILLSRISETLDLLYGAFYLADDSHTRFTRVGAFATDVSTEPRQ
                     FALGEALVGQAAAERRSLQIVPGADKPVEVSVGGVKVTPACILFVPVMNQDAVLAVVE
                     LAPSALVSERQQALLDALLPTVALNTKILVSKVETGRLLEQTQVQAADLAVAKEAAEA
                     ATKAKSDFLANISHEIRTPMNAIIGMTHLALKTDLSPKQADYLTKVKSAAQALLGIIN
                     DILDFSKIEAGKLDIEKTDFRLEAVLDNLSTIVSQKAQDKNLEFLIAAQHDIPPNLVG
                     DPLRLGQILINLVNNAVKFTDRGEVLVTVALEERLADRVKVKFSVRDSGIGMTPEQSA
                     RLFQAFAQADTSTTRKYGGTGLGLSISKRLVEMMEGTIWAESEPGVGSTFHFTAWFGI
                     GSEDKHKRFIPDLAGIRALVVDDNAQAREILTEALRVFALRADSVASGEDAVREIAAA
                     DSQDPYRLVLMDWRMPGMDGLQASGIIKRNDRLQHIPKIVMVTAFGREDIRTKAEEIG
                     IDSYLLKPVNQSLLYDTLVDLFGVAGLEDHRVRARKDDAPVHDATGIRVLLVEDNEMN
                     QQVATELLESAGAIVTVANHGGEAVKILTEGDQAPPFDVVFMDLQMPEMDGFTATKLL
                     RRDPRLKKFPIIAMTAHALVEERQRCLDAGMNDHVSKPIDPDNLFSTLMRWAKPQPKE
                     AVESQASPLPAKATDEVLLPEIAGVNLADGLKRVAGNRRLQRDLLRQFAEKQGDAAAQ
                     ISTALESGDKSLAERIAHTVKGVAGNIGITEVQSAAQKVEKAIREGQDSVPALLGEFA
                     GLMKTQVNAIEQALRDTAATQPEEASPAPFSEEAAVAAIVRLRTLLEASDGNAEEAFR
                     SLHAAVAGTVEKPQLDALNASITDFDFEAALLKLNQIAEHCAPNGEHAK"
     gene            6688..7095
                     /locus_tag="VFC15_04890"
     CDS             6688..7095
                     /locus_tag="VFC15_04890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09528.1"
                     /translation="MPSNPRFLPVVRAAVGELSLVYGLPEESSQGVTLAVDEALANII
                     RHAYKNRYDQEIELNCQAGTDQIEFTLLDQGEPPDPARICGQPLDDVSLSGRGTHLIK
                     AMMDEVSYERVPGGNQLKLIKHLPTAKVSVDGE"
     gene            7219..7545
                     /locus_tag="VFC15_04895"
     CDS             7219..7545
                     /locus_tag="VFC15_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM09529.1"
                     /translation="MKSSTRIEKGITIVDVEGQIDLGSSPALRKTLLASLQGTERVAV
                     NLMAVKYIDSSGIASLLEVLNAARQSKKRLVLFGLNGAVLQVLQLTRLTGVFDILETE
                     EQALEA"
     gene            7606..8418
                     /locus_tag="VFC15_04900"
     CDS             7606..8418
                     /locus_tag="VFC15_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09530.1"
                     /translation="MSGKIAAVPELVGFWTLNGLAYMGGLANLAGQSAYITFVGPFRG
                     QRLRPQRAIHQAMAVGVEAIPIVSLITFFVGLIMALQSAYQLRQLGAMHFVAQAVAIS
                     ILRELGPLLTAIVVIGRSGSAFAAEIGTKKVTEEVDALRTMAFDPVAFLVAPKFLAML
                     VMMPCLTIWADFMGVVGGSVFGVAGANFTFGSYFQATRDAIVLRDMYTGLIKSVLFGL
                     VITAVGCKEGFSTGAGAEEVGRSTTAAVVTSIALVIVVDLVFTGLFYVINPT"
     gene            8525..9346
                     /locus_tag="VFC15_04905"
     CDS             8525..9346
                     /locus_tag="VFC15_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM09531.1"
                     /translation="MSDSGAGNPAISVVNLRVSYGDREILHGISFDVQRGETMVILGG
                     SGSGKSTLLRTLVGLEKPTKGEVWITGINTAQISQREMDAIRKKIGMSFQGSALFGSM
                     TVGENVALPLREHTELEDSTIEVMVRIKLGQVGLSNFQDYMPSELSGGMKKRAAVARA
                     LAMDPEILFFDEPSAGLDPIIAAGLDELILNLKKAYAITMVVVTHELASAFLIADRMV
                     LIDRGHIVALGTKEELQASTHPRVRQFLDRVPEPEVTEELDYLQMLTGDRTAAKS"
     gene            9376..10344
                     /locus_tag="VFC15_04910"
     CDS             9376..10344
                     /locus_tag="VFC15_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZM09532.1"
                     /translation="MNSKREQAWVGLFVLIAAALLIGTVLAVAGTFSVSGVSHHAYFK
                     SAGGLLPGATVRYAGMKAGRVEAVRVDPQDSTRIEVAFTVGHAIPVKTDSVAKITSLG
                     PLDENYVEVGTGTKEAPLAPPGSEVKSVATVGIADLGDMIGNLAPVAQQVLQNLNQRL
                     TELQVTVARVNDLLNDRNRTNVSEGLGTLNAMLAEDRPKVSATLTNVQTASAKIIPLL
                     DDLKTTMKQANDALSHIDSVVLENRQDIRTAVVELKQTLLSASSLMEQLKNTMDRNTD
                     NIDEILVNLRITTENAKQLTEALKTRPSVLIRGDTVKDRKPGEKVN"
     gene            10489..11100
                     /locus_tag="VFC15_04915"
     CDS             10489..11100
                     /locus_tag="VFC15_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZM09533.1"
                     /translation="MHRIARPILISLLALGLSGCGAGRPIRYYTIQLPVAPSHSTNTY
                     PVSLLVASISGPQIFQDTPIAYRIGTNEIGTYQYSRWAEPPVEMLKGKLIRVLGASGD
                     YQSVASLGSTSEGQFVVRGRLYDFEEVDRGGIAGLVSMEFELYDRKSGKVVWSHFYSQ
                     SEPVQSKEISAVVTALDVNLDRGLKEVAAGLNQYFSANLATKS"
BASE COUNT         2325 a         3202 c         3201 g         2555 t
ORIGIN      
        1 gtcctaactc gcccaatggg tttgagctcg catcttggga gctcgatatg agcgataatg
       61 aaaaccgcga agacctgatc cgccgaagat gggccgaaac tggcagcaag ctctggaagc
      121 acgacttcca tggtgagggg ctcgcacccc tgaatattca gggattgctg gacaaagcgg
      181 tccagcacgg ttgggatgcc aagctttcgc acccctccgc catccggctt cgggatttcc
      241 acccgcctca ccggtttcgg ttcgtagatc ccgctcagca attgctcccg aatgaccggc
      301 cagtgctgct tcaggtagtc accgagttta tcaacggtca tcccatcgac gccggcgctg
      361 cctttgttgc tcctcacttg ccgcaatgct tccttcaagt ttgctcgttc acatatttcc
      421 tccttgattc gacttgcgtt ggctgggcgt tcgggttcaa gcaccaccga gagcgattcg
      481 atgtcttcgc gtcccgcctg ccgggcttca cccgtcggcg tcgatgagaa gtccagtctc
      541 aactggatgt tcagccgttt gtcgctctcg agattcatgt cctacttgcc tctcctctcg
      601 ttcgggcctt cagccatcgt ttccggttcg gcctatctgt tgactccacc tttcggcatg
      661 gagtgcctca atagcctcgc cgacgacatg acccactatg ccctctgctg acttctgccc
      721 tgcggtcaga ccgcctcacg gcgccctcag tcgccgaagc gacacagagc agatctcctg
      781 gggtaatctc agtcgccttc cgtgcaccat cgccgggttt acgcttcgca tctttgatgg
      841 atatggactt tgcggtgagt ggcccgctcg tccgatgctc gcgccttgta cccgatgttt
      901 gtccatcgac tcgcacgttt gttccatgct tccttcagac cccgcctcgc ggcgacagcc
      961 cttgcatcat cactagacct tacctccatc aggtaggcca cccggcgcga aaaatgtact
     1021 gcaaactcga acgattcttg cgtacactgt gtcattagaa ggccgtccct ttcctggcat
     1081 cgacttcgtc gagataacgc agttatgcct gagatgacgg cctttttcca gctctaaatc
     1141 acaaatcttg tttactgctg gtgagaaatc cgggctaacg tgtcgggcca acctccggtt
     1201 ggcccgacaa ctcaccggct gagtggtccg cgcagattgt tgggaatttc tttggtggga
     1261 gagcgaaccc gccgggtgct acctcagtcg ccttttacgt ggagtgccac cagcgtaata
     1321 tcgtcgtcca actcttcgtt cgggccgcgg gccgaccaga gggacaactc ctcaagcagt
     1381 cgatcggcaa agtgatccgc cgaggtgctt tcctccttcg ccagaaactc ccggaagcgg
     1441 tctgtgccaa actcgattcc caatgggttc gttgtttccg agattccatc ggtgtaaagc
     1501 aaaccccaat ggcctggctg gagcggcaac tccacggaag aataggtagc aaactcgaat
     1561 gctccgagaa acaagccatt ttcttctaca gcgcgccctc cgtcggatga accaccccac
     1621 aagagcaacg gagggtggcc cgcgcctgca tacgtcagcg ttcgcttctg caggtccaca
     1681 aacaagtatg ccgccgtgac gtaatggtgc tggaactttc cgcacagcgc ttgattcagg
     1741 cctaacagca cttgcgccgg atcggcagcc tgcgccacct gggctgagag tgcaatcttc
     1801 agcatcgagg caatgagcgc ggcaggcatt ccgtgtccgg acacatccgc taccagaatc
     1861 ccaatgtgtt tctcatccac gactatgaag tcgtagaaat cgccggccac cgaggtcatg
     1921 gggacgtagc gcgcagcaat gtccagacct tcgattttcg ggatctggga tggaagaatc
     1981 gacaattgga tttgccgcgc ggtttccatt tccttttgga tcgccagcaa ctgtcgcgcg
     2041 agctgttcgc gaatcccgcg gagcatgaga tgagtctgga cccgcgcctt tacgactgca
     2101 ggagaaaacg gcttatgaac gtagtccacc gcaccggcat cgaaccctcg tgtctcatcc
     2161 tcgacttcag tctggccggt aagaaagatg actggaatat cccgagtatc cgggtcagct
     2221 ttcaattgcg cacacacttc gtatccgtcc atctcgggca ttatcacatc cagcaggatg
     2281 aggtcgggcg ggggagtagc tttcgccaac tccaatgctt ttgcgccatt ggtggcgatg
     2341 cggattttgt agatgtcctt caggatcgag ttcacaacct ggatgttggc gggagcgtca
     2401 tccacgatca acactgtctt cttcgagtca gcctgattca tttcgcgtgt tccccatttg
     2461 gtgcgcaatg ttcggcgatc tgattcagtt tcaacagcgc tgcctcaaaa tcgaagtcgg
     2521 tgatggatgc gttgagagcg tccagttgcg gtttctcgac ggtgcctgcc actgcggcgt
     2581 gcagactgcg aaaggcctct tcggcattgc cgtcgctggc ctcgagcagg gtcctgagcc
     2641 ggacgatggc ggcgactgcc gcttcttcac tgaaaggcgc cgggctcgct tcctccggtt
     2701 gagtagctgc tgtatcgcgt agtgcttgtt cgatagcgtt aacttgagtc ttcatcaggc
     2761 ccgcgaactc gccgagcaga gccgggacag aatcctgtcc ttcgcggatt gccttctcca
     2821 ccttctgggc ggcggactgc acctcggtga ttccaatatt tccggcaaca ccctttactg
     2881 tgtgcgcgat gcgttccgcc aggctcttat ctccactttc gagcgcggtt gagatttgcg
     2941 cggctgcatc gccttgtttc tccgcaaact gccgcagcag atcgcgctgg agccgcctgt
     3001 tacccgcaac gcgcttcagg ccgtcggcga gattgacccc agcgatttca ggcagaagca
     3061 cctcatcggt cgcttttgcc ggcaggggtg acgcttggga ctcgactgct tccttgggct
     3121 ggggcttcgc ccatctcatc aaggttgaaa ataggttatc gggatcaatc ggtttcgata
     3181 catggtcgtt catgcctgcg tccaggcagc gctggcgctc ttccacgagg gcgtgcgccg
     3241 tcattgcaat aatcgggaac ttcttcaagc gcggatcacg ccgtaacaac ttggttgccg
     3301 tgaacccatc catttcgggc atctgcaaat ccatgaacac cacatcgaac ggaggagctt
     3361 gatcgccttc agtcaggatt ttcaccgctt ccccaccatg gttggcaacg gttactatcg
     3421 ctccggcgct ctccagcagt tccgtcgcaa cctgctggtt catctcgttg tcttcgacca
     3481 gcaggacccg gattcccgtg gcatcatgca ccggggcgtc gtccttcctc gcacggacac
     3541 ggtggtcctc gagtcccgca acaccgaaca ggtcgacaag agtgtcatag agcagcgact
     3601 gattgacagg cttcagcaaa tagctatcga ttccaatttc ctctgccttc gttctgatat
     3661 cttcccgccc gaatgcggtc accatcacga ttttgggaat gtgctgcaga cgatcattgc
     3721 gcttgataat gccgctggcc tgcaggccgt ccattcccgg catgcgccaa tccatcaata
     3781 ccagccggta gggatcctgg gagtcggcag cggcgatttc gcgtacggca tcctctccgg
     3841 acgcgaccga gtcggcccgt agcgcaaata cccggagcgc ctcggtaaga atctcgcgcg
     3901 cctgcgcatt gtcatcgacc accagcgccc ggatgcctgc aagatccgga atgaatcgtt
     3961 tgtgtttatc ttctgatcca atgccgaacc aggcggtgaa atggaaggta ctgcctacgc
     4021 caggctcact ctcggcccag atggtgccct ccatcatctc aaccagtcgc ttggagatgg
     4081 agagtcccag cccggtgcca ccgtactttc gcgtcgtcga agtgtctgcc tgcgcgaaag
     4141 cctggaacag ccgagcgctc tgctccggtg tcatgccgat cccgctgtcg cgcactgaga
     4201 acttcacttt gactcggtca gcgagccgct cctccagcgc aaccgtcacc aacacttcgc
     4261 cacggtccgt gaacttgacc gcgttgttaa ccaggttgat caggatctgc ccgaggcgca
     4321 gcgggtcacc gaccagattg ggcgggatgt catgctgcgc tgcgatcaga aactcgagat
     4381 tcttgtcttg agccttctga ctgacgatcg tcgatagatt gtcgagtacg gcctcaagcc
     4441 ggaagtccgt cttttctata tcgagcttgc ccgcttcgat cttcgagaag tcgaggatgt
     4501 cattgatgat cccgagcaac gcttgtgcgg cggatttcac cttggtcaga tagtctgctt
     4561 gtttcggcga tagatcggtc ttcaaggcca ggtgtgtcat gccgatgatg gcgttcatcg
     4621 gcgtacggat ttcatggctt atgttggcca ggaaatcaga tttggccttg gtggcggctt
     4681 cggccgcttc tttggctacc gccaggtctg cggcctgtac ctgggtctgc tccagcagcc
     4741 tccccgtctc gactttgctt acaagaatct tggtgttgag cgcaactgtc ggcaatagcg
     4801 cgtcgagcag tgcctgctgc cgttcggaca ccaatgccga aggcgcaagt tcgacgactg
     4861 ccagcacagc gtcctggttc atcacaggaa cgaacaggat gcaggccgga gttaccttca
     4921 caccaccgac gctgacttca acgggtttgt cggctccagg aacaatctga agactgcgcc
     4981 gctcagcggc agcttgcccc accaacgctt cgccaagcgc aaactgtcgc ggttccgtcg
     5041 atacgtccgt cgcgaaggcg ccgacgcgag tgaagcgcgt gtggctgtcg tcggcgaggt
     5101 agaatgcacc gtacagcagg tccaacgttt ccgagatccg tgatagcagg atggtggcga
     5161 atgccggaaa gttttccgcc gactgcagtc tctcagtcgt ggccgccacc tcagctttga
     5221 cccagcccaa tatctcccgc tcacgcgccg ccatctggag ggtctgcagc gcacggctca
     5281 tctcgccaat ctcgttggaa cggttcaggt tcgcaacggg aaggtccagt cgtccctctg
     5341 ccacgtccag aatccggcta cggaatgaca acacaacctt tacgacttca acctgcacga
     5401 tggagagcgc aacgacgaaa gatacgacca agccgagcac gatcacaatc catgtgatca
     5461 gaatcgcgcg gtgcgtccgg gaagtgagtt cgtcggattc ctggtttgca gacttttgca
     5521 tctcgtccac caggtcggtc atagactgcc ttgcctgctg caaatcagcg tcgaccccgc
     5581 tgcgcatgat gttcatggcc ttttcacggt tgccagccaa ggcagcggcg cggacggggc
     5641 gtgcactgga tgcagctcga tcgaacacag tctctgccgc tttgatttcg ctagcgcgac
     5701 cggggttctg ccgtaacgcg tcggcgagca atgcccggta atcggcttgc accttgtcca
     5761 attctccgtc gttttcctgc ctttgatcgg ggttgtcctc ggcgactagt tggtagagca
     5821 acaaacggaa ccgcatcgtg ttcccccgcg ctgcggtcac cgattgaaat gtcttgacgt
     5881 aattgttgat caaattgctg taccacgtgt cgatcttctg gctttcgatc gacgaatata
     5941 cccccgcccc aatcgccatc agcgccagcg gaagcatggc gactagcacc ttggtccgga
     6001 ttttgaaatt cgctaggaga cccatctcaa cggtacctgc cccaatcgcc gggcgggtgt
     6061 acccgacaat cgccaattgt acctctcaca ctgcgtctca tttgtgacgg gtgcaagctc
     6121 gtgtgggctt cccattttgt ccaacttaag ttgtactgcg ataggagcga aataagctca
     6181 tagttgagac gtgtatgcca atccgcttgg gcttcttgtc caaaacataa tggggccggt
     6241 atcggggcaa gtcgatgatc gtttgcgtgt acagggtctt gggtcataac ccgatatttc
     6301 ggggcgacct ccggaatgtc gggtatttcc ctgtcccgga aaatctgaca cgtaggcaat
     6361 agctgcgttt tcagttgatt aggaaggccg cagcccttcc gtttgttggc atgcagattg
     6421 ctttgattaa tacagcagga gcaacaagaa gcgagaggga gggacatgtc ggcgccagga
     6481 tgagtgtata aaagcagtag ttgagcatgc ctgctcgagc gtgtggagat ccggacaggg
     6541 cacatctata ggaccgaact ggagaacgcc agcgccaaat gatggtatca tctagcgccg
     6601 ctgatctcga caagaggaat ggcacctgat gttccactgc aggaacagtc ttagcggagc
     6661 caaatgagct ttcaattgag gcttgttatg ccaagcaatc cgcgattctt gcccgtagtc
     6721 cgcgctgcag tgggcgaatt gagtttggtt tacgggctgc cggaggagtc ctcccaagga
     6781 gtcactttgg ccgttgacga ggccttggct aacatcattc gacacgccta caagaatcgt
     6841 tatgaccagg agatcgagct gaactgccag gcgggtaccg atcaaataga attcacactc
     6901 ctcgatcagg gcgaaccgcc ggacccggcc aggatctgcg gccagccctt ggatgacgtc
     6961 tccctgagcg ggcggggaac gcacttgatc aaggcgatga tggatgaggt gtcttacgag
     7021 cgagtcccgg gggggaacca actgaaacta atcaagcacc tgccgactgc caaggtgagt
     7081 gtcgatgggg aatgatctgc gcctcagcga ttgcgacaaa gcctgaacat ggctgaggcg
     7141 ttgatagtta gcgttcgtga aggttgacaa acaggtgaag gcttttgtgc ctagtgaagg
     7201 atggacatgg aaagatactt gaaatcttcg acgcgaattg aaaaggggat cacgatcgtg
     7261 gatgtggagg gtcagattga ccttggtagt tcaccggcct tgcgcaagac actgctggcg
     7321 tcattgcaag ggacggaacg tgtggccgta aacctaatgg cggtgaagta catcgatagc
     7381 tccgggattg cgtccctgtt ggaggtcctt aacgcggcgc gtcaatccaa gaagaggctg
     7441 gttctatttg ggttgaacgg cgccgttctt caagtgttac aactcacgcg cctgactgga
     7501 gtttttgaca tactcgaaac cgaggagcag gcgctcgaag cttaaaaccc tgccttcgaa
     7561 gaccggtgaa gcaattccct gaaacggggt ccgttgacgt tgaaagtgtc gggaaaaatc
     7621 gcagctgtac cggaactggt cggtttctgg acgctgaacg gcctcgccta catgggtggc
     7681 ttggcgaacc tcgcgggcca gtcagcatat atcaccttcg tgggtccttt ccgaggtcag
     7741 cgcctgcgcc cccagcgggc gatccatcaa gccatggcgg tgggcgtcga agccataccc
     7801 atcgtttccc tgatcacgtt ttttgtgggc ttgatcatgg ctctgcagag cgcatatcag
     7861 ctgcggcagt taggagcgat gcattttgtg gcccaagccg tggccatctc gattcttcga
     7921 gaactcggtc cactgcttac agccatcgtc gtcattggtc gttcggggtc cgctttcgcc
     7981 gcggagatcg gcaccaagaa ggtgaccgag gaagtggatg ccctgagaac aatggctttc
     8041 gatccagttg ccttcctggt cgcgccgaag ttcctggcca tgctggtgat gatgccctgc
     8101 ctcacaatct gggctgattt catgggggtt gtgggcggaa gtgtcttcgg tgtcgccggc
     8161 gccaatttta cgttcggctc gtattttcag gcgactcgag acgcgatcgt gttgcgcgac
     8221 atgtacaccg gcctcatcaa gagcgtgttg ttcggcctgg tcatcacggc tgtcggatgc
     8281 aaagaaggct tctcaaccgg ggcaggcgcc gaggaggttg gacgatcaac gacggcggcc
     8341 gtcgtcacct cgattgcact cgtgattgtt gtcgatctgg tgtttacagg tttgttctat
     8401 gttattaacc cgacttagca catgagttcg cacatgatgt cacagtatcg gccgaccctg
     8461 gaatcagaac cgcgtgtaga tccgaatctg gtctgcagac cggccgctgt cggaggtcct
     8521 gaagatgagt gactccggcg ccggcaaccc agcgatttcg gtggtcaacc tccgcgtgag
     8581 ctacggcgat cgcgagatcc ttcacggcat tagctttgac gtgcagcgtg gcgaaaccat
     8641 ggtgatcctg ggcgggtcag ggtcaggaaa gagcactctc ttgcggacgc tggtcggttt
     8701 ggagaagccg accaagggcg aggtgtggat tacaggcatt aacaccgcgc agatttccca
     8761 gcgcgaaatg gacgccatcc gaaaaaagat cggtatgtcg ttccaaggct cagccctgtt
     8821 tggctccatg accgtaggcg aaaacgtcgc gttgcccttg cgcgagcata ccgaactcga
     8881 agactctacc atcgaggtca tggtgcggat caagctcgga caagttgggc tgagcaattt
     8941 tcaagactac atgccttcgg aactcagtgg aggaatgaaa aagcgcgccg ctgtcgcgcg
     9001 tgccttggcc atggaccccg agatcctgtt tttcgatgag ccgtcagcgg ggcttgaccc
     9061 catcatcgca gcgggactcg acgaactgat cctgaacctc aagaaggcct atgccataac
     9121 gatggttgtg gtgacccacg aattggccag cgcttttctg atcgctgacc gtatggtctt
     9181 gatcgaccga ggccacatcg tggcgctggg aaccaaggag gaactccaag cgagcactca
     9241 tccgcgcgtg cgccagttcc tggaccgggt accggagcct gaagttaccg aggaactcga
     9301 ctatctgcag atgctcaccg gcgaccggac ggccgcgaag agttgagaga gtggagcctt
     9361 tgaaatgagg atgtcatgaa tagtaagcgc gagcaggctt gggtaggact ctttgtactg
     9421 attgcagcgg ctctgctgat tgggactgtc ttggccgtag ccggcacttt cagtgtgagt
     9481 ggggtttccc accatgcata cttcaagtcc gctggcggtc tcttgcctgg cgccacggtc
     9541 cgctacgcag gcatgaaagc cggcagggtc gaggccgtgc gcgtggatcc tcaggactcg
     9601 acccgcatcg aggtcgcttt tactgtggga catgctattc ccgtgaagac cgacagcgtg
     9661 gccaagatta ccagtctcgg acctctcgac gagaactatg tggaagtagg aaccgggacg
     9721 aaggaagccc cgctggctcc gccgggcagc gaagtcaagt ccgttgcgac ggtcggaatt
     9781 gcggatctcg gcgatatgat tggcaacctg gccccggttg cccagcaggt tctgcaaaac
     9841 ctgaaccagc gcctcacaga actacaggtt acggtcgccc gcgtcaatga cctcctgaac
     9901 gaccggaacc gcacgaatgt cagcgagggg ctggggaccc tgaacgcgat gcttgccgag
     9961 gaccgcccga aggtttcggc gaccttaacc aatgtgcaga ctgcgagtgc caagatcata
    10021 cccctgctcg acgatctcaa gacaaccatg aagcaagcta acgatgcctt gtcgcacatt
    10081 gattctgttg tacttgagaa tcgccaagac atccgtaccg cagtcgtcga gttgaagcaa
    10141 acgttactct cggcttcatc gctcatggag cagcttaaga atacgatgga tcgcaacacg
    10201 gacaacattg acgaaatact ggtgaatcta cgaatcacaa ctgagaacgc gaaacagctg
    10261 acagaggcgt tgaagaccag gccttcagta ctcattcgtg gagacactgt gaaggaccgc
    10321 aagcctggtg agaaagtaaa ttaggacctt tccgccggga cataaccgat agccgtattc
    10381 agtggcttga tccctacaga gggcggcgag aaagctttag gcaacgcggg agtagattaa
    10441 attcaagacc ttggatcgat atcgaaaggc acacggaaga ggttctccat gcatcgaata
    10501 gcccgtccta ttctgatctc tttactcgcc ctgggcctga gtgggtgcgg cgctggccgg
    10561 cccattaggt attacacgat tcagcttccg gtggccccat cacattcgac taacacctac
    10621 ccggtgtctc tgttggttgc aagcatcagc ggaccgcaaa tctttcagga cacgccgatc
    10681 gcgtatcgca tcggcacaaa tgaaattggc acataccaat acagtcggtg ggcggagccg
    10741 cccgtcgaga tgcttaaggg caaattgatt cgcgtgcttg gtgcctcagg tgactatcag
    10801 tccgtagcca gtctggggag cacgtcggaa gggcagtttg tggtccgagg caggctctac
    10861 gacttcgagg aagttgacag ggggggtatc gccggactcg tgtccatgga gtttgaactc
    10921 tatgaccgaa agtcgggcaa agtcgtctgg tcacatttct attcccagtc cgaaccggtg
    10981 caaagtaagg agatttccgc ggtagttacc gcgttggatg tcaatcttga ccgcggactg
    11041 aaagaggttg ccgctggact caaccagtat ttttcggcaa acctcgccac gaagtcttga
    11101 gggtatttga tttgaatcgt tccgggtctc aagggacgcg gtagactccc tgatccacga
    11161 gcgtaaggcc cagggtcgcc gaccgcgcca gtcagcgata aaagcagatg tggaggtccg
    11221 tgggtgctcc atcctttcgc caccttggga atgtcgagtc tccgagtttt gggtgacaag
    11281 cct
//
LOCUS       DASRKK010000230        12569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_602874, whole genome shotgun sequence.
ACCESSION   DASRKK010000230 DASRKK010000000
VERSION     DASRKK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602874"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFC15_04920"
     CDS             <1..842
                     /locus_tag="VFC15_04920"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM09534.1"
                     /translation="TPKSKIQQYEKKWLDLPWQKVRESVEVKLFEQEGELYVLAKSDG
                     RRAKETAMRRQRLARLLRKLRAMRRSLPSRDQLLMRLGAAKTAAGRAFYFVQMQVPGK
                     DQPVTRETFQFRVDKKKLQEAEWRDGHYLLRSNLTAGDPSVLWTRYVQLTQIEAVFRS
                     LKSELGIRPIHHQLEHRVDAHILIAFLAYCLQITLKQRLLLHAPGLTPTAVLEKLAEI
                     QMIDVWIPTVDQRWLILPRYTQPSSDTKLLIEKLRLQLPSQPSPRLTEQNESTEIEIQ
                     TVQ"
     gene            complement(1058..1303)
                     /locus_tag="VFC15_04925"
     CDS             complement(1058..1303)
                     /locus_tag="VFC15_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09535.1"
                     /translation="MDEREANQEAKAELGRRILAALMSYQLHLKSVDYTMKTYLSNQE
                     VDPSWGELGWTLLEMMNRGLAEKTLGPALRSLTAKIQ"
     gene            2374..3390
                     /locus_tag="VFC15_04930"
     CDS             2374..3390
                     /locus_tag="VFC15_04930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM09536.1"
                     /translation="MRARSLLCISTAFCLTLIAHSVAQARDSLPSWNDGPAKQAILTF
                     VKVTTDPASPSFVPPEQRIATFDQDGTTWVEHPMYTQFIFAFERVAELAPQHPEWKTT
                     PPFQAVLTDDKAAMAKFTMKDLETILVATHTGMTVDQFQDIVKDWLAKAKDQRWHRPY
                     TDLIYQPMLEAMQYLRDNGYKTYIVTGGGQDFVRVYGEQVYGIPPEQVIGSALETQYI
                     YNKEGQGILMRPPKLLLNDDFSGKPEDIYLFIGRHSKVAFGNSTGDQQMLEYTQAGGG
                     APLMMIVLHDDAQREYAYGPAQGMPDTKVGTFTQALYDEAKSKGWFVISMKDDWKRIF
                     AWEK"
     gene            3848..5476
                     /locus_tag="VFC15_04935"
     CDS             3848..5476
                     /locus_tag="VFC15_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM09537.1"
                     /translation="MKTARLSKMSNWWTLAVLIVVVLVSKLGFAQTKKPNIIILMTDD
                     VGWMDLGVYGGGADRGAPTPNLDRIAAEGMRFTEYYAQPTCTLGRLAVMIGREPVRAG
                     VLNVFIPGDPNGINGAEVTLPQYLKKAGYHTMQTGKWHLGDKPVYYPTQHGFDEMHYM
                     LPYYGNVYTYDDTSFYPDFPSNDPKFMAYWSKMNLKMWEQNPGAKEATAMLNGREFRT
                     AELKLVDQWQTEFVTQWIKQHAKDTEPFFIDMNFMRMHNPSVIPDEDLRKSPGQFPYT
                     DGLMILDRQAGQVMAAIKEAGIDDNTIVVWTTDNGAWIDTWPDAGETPFRGEKSSTFE
                     GGYRVPAMVRWPGHVPAGVVSHDIFSCMDWFPTLAVQAGLPAPPRHWVDNSGTKIVFD
                     GLDQSDVILGKGPGKRDSVLYFSAGGGIAVRQGRWKLTYNSHDSWLGVNRNMTAPALY
                     DLLTDPFELHDIVFMGSAPMGDAMKTSPGRSPEHDHGWILPSLAIVLRNTFAEMQEYP
                     NRFLGLDGEVYWEALPELPKNLSVPPKGVKIPED"
     gene            5807..6172
                     /locus_tag="VFC15_04940"
     CDS             5807..6172
                     /locus_tag="VFC15_04940"
                     /inference="COORDINATES: protein motif:HMM:NF023170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HZM09538.1"
                     /translation="MTFLDVFSLLILLFLILLGVGLILLLAYTPGAIARSRHSPWAEA
                     INIAGWVGILVFPIWIVALAAAFIRPRTGEGAAIAISEAETAELAATISSISERLKTL
                     ENGLRTVLPKAASSGPGGA"
     gene            6175..6393
                     /locus_tag="VFC15_04945"
     CDS             6175..6393
                     /locus_tag="VFC15_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09539.1"
                     /translation="MLVAILVICYVLVLRYLDQKNREKDRIKSGVAIPSGPADYGTIK
                     KALAETSERLAVVEGQYRQLARRLGVTQ"
     gene            6390..7613
                     /locus_tag="VFC15_04950"
     CDS             6390..7613
                     /locus_tag="VFC15_04950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM09540.1"
                     /translation="MIAFLAVVYCVGLYLVFIKFRLLPFNLLAQILVGGVGFIGLLVV
                     LFGMNYTQPFSIGTTVSGLTTQIEARVPGKVIEVDVKDDSFVKKGEVLFRMDPQPYVD
                     QLHHAQSAYAEAEIRTSTAILQTTQTVNSASAQVQAVEAQIKATQAAIGATESQLNLA
                     RTRLKEYTELKSKNAGSLFEVERYETDVKSLTDQVAAQNQQMAAQKQQLASAQAQLVQ
                     AQTALQEAIKIQPDILSGLNAEVTGAQWSVDQTTIVAPDDGYVTQVTLQPGTMVSIGP
                     VMAFIGQRTKPLLVVTVMQNYVNVVKPGAKAELATPALPGKILHARVLAVERATGSGA
                     LYPEGRLKRAFEPAFPDRMYVILNLDDELKDTILPIGTNGWISIQGKQWSELFIIRRV
                     IMRWYTWTNYIFTGY"
     gene            7628..9055
                     /locus_tag="VFC15_04955"
     CDS             7628..9055
                     /locus_tag="VFC15_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM09541.1"
                     /translation="MNTSVRFQKLVSGVAVFGLVVLLCACAVKKPPLTTQVVHDSLPP
                     TTTIPDQWTSTPVNPAPVETGWLKSFHDPQMEAIVAEALRNNLDLQAAATRVQVAANV
                     VTEVHAQLMPQIGIAGEAKYLGRFHEKNATGKAIGNFNASSLLGGVSWELDVWAKIRS
                     QVAAAKEGQAATGSDYQWARMSLAAITARVWYLAGYTGVLQSYAQQNVAIDQKLLYVT
                     IAQFQVGNAEQQQVDFAQAQLAQSQAQLAQVSNAYLQIVRGLEVLLGRYPAGELKTAS
                     ALAALPPPAPAGLPSQLLERRPDVLAAQHDFNAAFHLVQTAKAARLPSFALTGAGGYL
                     TNEIFQDLKFRPWVWTVAGNMIAPLYTGGYLQAQVKISNENQKAALALYGQALLQAFD
                     DVEVTFTNERYLKDQEKDATEALKSTEDALNLAKVKYEVGQTDLSPVLQLESVVLATQ
                     TADTYLQYELIANRIDLYLALGGPF"
     gene            9363..9602
                     /locus_tag="VFC15_04960"
                     /pseudo
     CDS             9363..9602
                     /locus_tag="VFC15_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9688..10641)
                     /locus_tag="VFC15_04965"
     CDS             complement(9688..10641)
                     /locus_tag="VFC15_04965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09542.1"
                     /translation="MEKNQEHENAAGLVPHRPLGSTGIRVSAMALGGYHLGSAKDQQE
                     VNDIVACAMDAGVNFLDNAWEYHNGHSEEVVGIALKGKRQQAIVMSKVCTHGRGKDIA
                     IQQLEQSLRRLQTDYLDLWQVHEMIYENDPDLIFRPGGAADALFEAKQQGKVRAIGFT
                     GHKDPSIHLKMLSHDFPFDTVQMPLNCLDATFRSFETSVLPVAKQRGMGVLGMKSMGG
                     SGEIIIHGAATPEQALRYAMSLPVSSTISGVDSMEVLEQNLALARGFQPMPEQQMQAL
                     REQCKFFASDGRYELYKVTKKYDGEVGREQHGFPTSEDLPA"
     gene            complement(10691..11698)
                     /locus_tag="VFC15_04970"
     CDS             complement(10691..11698)
                     /locus_tag="VFC15_04970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09543.1"
                     /translation="MEQDSASSGIPLRRFGNTDARISALGMGGHHLGDAPDEATAVRM
                     VHEAVDGGITFFDNCWEYHLGKSEIWMGKGLKGRRDRVFLMTKVCTHGRDGDLALQML
                     EQSLNRYQTDHLDLWQVHGVCFDNDPELFIRPNGAAEALHKAKEQGKVRFVGFTGHKH
                     PDIHLKMLATGFPFDAVQMPLNVFDSQFHSFETHVLPELNKRGIAALGMKPISGHGEP
                     VSRGVITVREALRYAMSLPVATTITGMESLDVLHQNLAIAQGFQPMSQPEMQALRERV
                     KVQAADGRFELYKMSLKFDNPEARLAHEFPVDVQQVEMKEMIQVTKNTGHPFSKIGSG
                     R"
     gene            complement(11811..>12569)
                     /locus_tag="VFC15_04975"
     CDS             complement(11811..>12569)
                     /locus_tag="VFC15_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09544.1"
                     /translation="PAGFPHSIQGLGPDGCEFLLVFDQGMFSEDNTFLISEWVAHTPP
                     SVLSKNFGLDESVLTKLPTDELYIFPAELPRSLDQDRAAVGGKKVQSPIQYTFKMQSM
                     PPTKKTAGGEARVVDSRNFPVARNIAAGLVTVKPGGIRELHWHPNASEWQFYIAGKGR
                     MTVFSPVGNARTMDFHANDVGYIPAVAGHCVENTGDTDLVFLEMFKASEFVDFSLNNW
                     IRRLPPEMVTSHLNLDEGEIKKIPAEKQSLIGRK"
BASE COUNT         2758 a         3616 c         3536 g         2659 t
ORIGIN      
        1 gaaccccgaa aagcaagatc caacagtatg aaaagaagtg gctcgatctg ccctggcaga
       61 aggtcagaga gtcggtggaa gtgaaactgt tcgagcagga aggcgagctg tatgtgttgg
      121 ccaagagtga cggaagaaga gccaaagaga ctgccatgcg ccgccagcgt ctggcccgct
      181 tgctgagaaa gctccgcgcc atgcggcgca gcctgccttc ccgcgaccag ttgttgatgc
      241 gcttgggcgc cgctaagaca gcagcgggcc gcgcttttta tttcgtgcag atgcaagtac
      301 ccggcaaaga ccagcctgtg acgcgggaga cattccagtt tcgggtggat aagaaaaagc
      361 tccaggaggc ggaatggcgc gacgggcact acttgctgcg ctccaacctt acggccggag
      421 accccagcgt gctgtggacg cgctacgtgc agctgacgca gatcgaagcg gtatttcggt
      481 cgctcaaaag cgagcttggc atacgcccca ttcaccatca actggagcat cgcgtcgacg
      541 ctcacatttt gatcgccttt cttgcctact gcttgcagat aaccctgaag caacgtctcc
      601 tgttgcacgc cccagggttg acgcccacgg cagtgctgga gaagctggcc gaaatccaaa
      661 tgatcgacgt ctggattcct accgtcgatc aacgttggtt gattctgccg cgctacacgc
      721 agccttcctc agacaccaag ctgctcattg aaaaactgag actgcagttg ccgagccagc
      781 cgtcaccgcg cctcaccgag caaaatgaga gcaccgaaat cgagatacaa accgttcagt
      841 aggcgccccg tttgtggtgg agacctttca atagcctcaa ctgattccaa agtacttaac
      901 tggatcagaa gcgcccaact gcgaaagatc ggttagtaag cgcactgtga gacggccgac
      961 tccatcggaa aatgtgagat taggtttcga cgatgaccgt gaatgctggc tccttaatct
     1021 ctgtgcgggc cagagttcaa agaggaagtc ctccgaccta ctgaatcttg gccgtcaagc
     1081 ttcgcagcgc aggtccgagc gttttctcgg ccaagccgcg attcatcatc tcgagaaggg
     1141 tccagccaag ctcaccccag gagggatcca cctcctgatt ggataggtag gtcttcatgg
     1201 tgtagtccac gctcttcaag tgtaattggt agctcatgag tgcggcgaga atcctgcgtc
     1261 ccagttcagc cttggcttct tggtttgcct cgcgctcatc cattgcgtac ccctttcgcg
     1321 ataaagaatc tcggttagac cgcgccggac cataagcccg actaaaaaac gaaacccagt
     1381 ttacattgca tcctctgcac ccggctcgac ggaattgttc gcctgtgcag agattcgatt
     1441 tcacgcttcc gtgcgagttg tgattcgaca tcctcaaggc ccgccttgtg ggccaattgc
     1501 cttttcggga gaggggtcgg caccgaaatg cctctcacgc atcaggaaag acgcgacttt
     1561 attaacaacc tgcaccagcg caaccggccg ccacgatgtt ccttcgcagg aatgatttca
     1621 tcgaactctc ggcttttcaa gaatggtggc aagaaatttt tgttgggacg ccagcctgcg
     1681 ccctgacgat atggaatccg tgttgccggc gtgaaaggaa aaccagctcg tatgtaattg
     1741 aaagcaaagg cgcgcaccgc cgcagctaaa gtcctaatca cctttggtcg cccggctgtc
     1801 gctgataaat gaaagccgcc tgcgagtcgg cggtgacatc ccaaatctgt aagtcactgc
     1861 ctgtcccccg ggttaaactg aaaatccatc acgggcccga cgcaacaagt gtcagtgagc
     1921 tgcgcatcag cgaccagctc tcgttcaatt gcgctctggt ttgaccgttc cctttattgc
     1981 tgtgctgcga tccggcgaga cctcttatca agagacgacg gcgccgcatt ttagcaaagc
     2041 tgtattcgaa ttccagaaat cgagctttca cacaggtcgc gctgccggat cttcaagacc
     2101 caatccggtt gttaccgaga ctaatagctg gttggtattg cggttggtac agctccgcct
     2161 cgctctatac gaccgaaggg ttggtcattg aattgcttgt atgagatggg cacccggagg
     2221 ctaaagacct gagcggggga gcgcaggcct tttcaaggaa gaaccactta gggggcaatc
     2281 tgttgatttg cagctagtga tgcttggagc catatccttg acttcaagct tgacgcggaa
     2341 atatccgata gcttcaacaa gggagacaaa ctcatgaggg cacgatcgct tttgtgtatc
     2401 tccacagcgt tttgcttgac gctgattgcc cactcggtcg cgcaggcgcg ggattctctg
     2461 ccctcgtgga atgatgggcc tgcgaagcag gcgatcctca ccttcgtcaa ggtcacgacc
     2521 gatccagcaa gtccgtcctt cgttccacct gaacagcgca tcgccacctt cgaccaggac
     2581 gggacaacgt gggtggaaca ccctatgtac acgcagttca tatttgcctt cgagcgcgtc
     2641 gccgagttag caccacagca cccggaatgg aagaccacgc cgccattcca ggcggtactc
     2701 accgacgaca aggcagcaat ggcgaagttc accatgaaag acctggaaac catcttggtt
     2761 gcgacccaca ccggcatgac ggtggaccag ttccaagaca tcgtgaagga ctggctggcg
     2821 aaggcgaagg accagcgctg gcaccgtccc tacaccgatc tcatttacca gcccatgctg
     2881 gaggccatgc agtacctccg agacaacggg tacaagactt acatcgttac cggcggtgga
     2941 caggacttcg ttcgcgtcta tggcgagcag gtgtatggca tcccgccgga gcaggtcatc
     3001 ggctctgcct tggaaactca atatatctat aacaaggagg gccaggggat cctgatgcgg
     3061 cccccgaagc tgctgctgaa tgatgatttt tccggcaagc cggaagacat ttatctcttc
     3121 attggccgcc actccaaggt agcattcggc aactcgacgg gcgaccagca gatgctggaa
     3181 tacacccagg caggcggcgg cgcacctttg atgatgatcg tgctgcacga cgacgcgcag
     3241 cgcgagtatg cttacggacc ggcccaggga atgcctgata ccaaggtcgg cacctttacg
     3301 caggcactgt acgacgaagc caagtcgaag ggctggtttg tcatcagcat gaaggacgac
     3361 tggaagcgga tttttgcctg ggagaagtga cccagcatta ccgaaggctt gaatggttga
     3421 actgcacact tcgcgcgggc gttggggggg gcgggctcat cgctctctct aactgattgg
     3481 atgacactgg aaaagattgc aagagtcatt gaacgctctt tttctagtgt cgcggcaaac
     3541 caaaaaaaag taaccggcat catcttgcag ttgattgaga gctcaaaacg attgcactta
     3601 aaaaactatt gcacttaaaa gaagatgcgt tgctatgaac acgcctgaga agccagtcgc
     3661 tgaagtttcg gttgcggatc cggcggccac caccgtaacc ggcgaggcta tctgggagcg
     3721 cgctggctta gacactgtat cttggtcgtt ctagtcgagg acctaaccag gcggcggtag
     3781 tgacactgtc caccattaat agcgcaaacc acggtcgcgg actaccagtt ctaaaggggg
     3841 agacacgatg aaaacagctc gattgtcgaa gatgtccaac tggtggacgc tggccgtgct
     3901 tatcgttgtt gtgctggtca gcaagctggg gttcgcccag acgaaaaagc cgaacatcat
     3961 tattctcatg accgacgacg ttggctggat ggacttgggt gtctatggtg gcggcgccga
     4021 ccgtggcgcg ccaacaccca acctggatcg catcgccgcc gaaggtatgc gcttcaccga
     4081 gtactacgcc cagcccacct gcactctggg gcgactggct gtgatgattg gccgcgagcc
     4141 agttcgtgct ggtgtcctca acgtattcat acccggcgac cccaatggca tcaacggggc
     4201 cgaagtgacc ctgcctcagt atctgaaaaa ggccggctat cacaccatgc agactggcaa
     4261 gtggcacctc ggcgacaagc cggtgtacta ccccactcaa cacggcttcg acgagatgca
     4321 ctacatgttg ccgtattacg gaaacgttta tacctacgac gacaccagct tttatcccga
     4381 ctttcccagc aatgatccca agttcatggc gtattggagc aagatgaatc tcaagatgtg
     4441 ggagcagaat cccggggcca aggaagccac cgcaatgctc aatggccgcg aattccgcac
     4501 cgcggaactg aagctggtgg accaatggca aacggaattt gtcacccaat ggatcaagca
     4561 gcacgccaag gacaccgagc cattcttcat cgacatgaat ttcatgcgca tgcacaaccc
     4621 atcggtcatt cccgatgaag acctgcgcaa gtcacctggc cagtttccct ataccgacgg
     4681 cctgatgatt ctcgaccggc aggccggcca ggtcatggct gcgataaaag aagccggcat
     4741 tgacgacaac accatcgtcg tctggactac cgacaacggc gcttggatcg acacctggcc
     4801 cgacgctggc gagactcctt tccgcggcga aaaaagcagt accttcgagg gtggctaccg
     4861 agtgccagcc atggtgcgtt ggcccggcca cgttcccgcg ggcgtggtca gccacgacat
     4921 cttctcctgc atggattggt tccccacact ggcggtacaa gccgggctgc ccgcgccgcc
     4981 ccgccactgg gtggacaact ccggcacgaa gattgtcttc gacggcctcg atcagtcgga
     5041 tgtcatcctg gggaagggtc ccggaaagcg tgacagcgtt ttgtacttta gcgccggggg
     5101 cgggattgcc gtgcgccagg gccgctggaa gttgacctac aactcgcatg attcctggct
     5161 gggtgtgaat cgtaacatga ccgcgccagc tttgtacgat ctgctcacgg atcctttcga
     5221 gctgcacgac atcgttttca tgggctccgc acccatggga gatgccatga agacctctcc
     5281 cggccggagt cctgagcatg accacggctg gatcctgccc agcctcgcca tagtcttgcg
     5341 gaatacgttt gcggaaatgc aggaatatcc caaccggttc ctcggccttg atggtgaggt
     5401 ttattgggag gcgctgccag agttgcctaa aaacctatct gtacctccga aaggggtcaa
     5461 gataccggag gattaaaccg gattcgttgc gacaggtggt gcggatgcaa tctgacccaa
     5521 gccctttaac tccagggtgc tttgttccaa agcgcggatt cgccggcaca tagatggcaa
     5581 tgcgcgtact ttgaggctcg aggaacttgg aaccgcagtt cggtactgag cactgcaggc
     5641 cttcgacgcg tcaaccctcg atggagagct ccgttcccgg tatagctgag tttcgcttag
     5701 gggacggacg ccaccttaat cggattgacg actgtacgtc tcaggtcgcc aaaacgcgtg
     5761 agaacgtgag gcgtctgcgt tagttagccc gcttggggag cgtgacatga cttttcttga
     5821 tgttttctcg ctgctgatac tcctctttct gatcctcctg ggcgtcggtt tgatcctgct
     5881 tctcgcatat acccccgggg cgattgcaag gtcacgacac agtccctggg ccgaagctat
     5941 caacattgca ggctgggtgg gaatcctggt gttcccaatc tggatagtgg cattggctgc
     6001 cgcattcatt cgtccgcgca ccggtgaagg tgctgcgatc gccatcagcg aagctgaaac
     6061 agccgaattg gcggcgacca tctcctcgat ttcggaacgc ctcaaaactc tagaaaacgg
     6121 gttgcgcact gtacttccga aggcggcatc ctcgggcccg ggaggagcat agccatgcta
     6181 gtggccatac ttgtcatctg ctatgtgctg gtcttgcggt atctggacca gaaaaaccgt
     6241 gagaaggatc gcatcaagtc tggagtcgcc attcccagtg gccccgccga ttatggaacc
     6301 atcaagaagg cattggcaga gacttcggaa cggctcgccg tagtggaagg ccaatatcgc
     6361 cagctggcgc ggcggctggg ggtaactcaa tgattgcctt tctggcggta gtgtactgcg
     6421 tcggcctcta tctggtgttt atcaagtttc gcctgctgcc gttcaatctc ctggcgcaga
     6481 ttttagtcgg cggggtgggc ttcatcggcc tgctagtggt gctctttggc atgaactaca
     6541 cccagccttt ttcgatcggc acaaccgtct ccggtctgac gacccagatt gaagctcgcg
     6601 tgccaggcaa agtcatcgag gtggacgtta aggatgacag cttcgtaaaa aaaggtgagg
     6661 tgctgttcag aatggatcct caaccttacg tggaccaact ccaccacgcc caatccgcct
     6721 atgccgaggc tgagatcaga acttcgaccg ccattctcca aaccacgcaa actgtgaact
     6781 cagccagcgc acaagtgcaa gctgtagaag cgcaaataaa ggctacgcaa gccgccatcg
     6841 gcgccaccga atcgcaactt aacctggcca gaacccggtt aaaggaatac acagagctga
     6901 aaagcaagaa tgccggtagt ctgttcgaag tcgagcgcta tgagactgac gtaaagtccc
     6961 tgaccgatca ggtggcggcc caaaatcagc aaatggcagc gcagaagcaa caactggcct
     7021 ccgcccaggc ccaactggta caggcgcaaa ccgcgctgca ggaagccatc aagattcagc
     7081 ccgatatatt gagtggcctg aatgccgaag tcacgggtgc ccagtggagc gtggaccaga
     7141 ctacgatcgt ggcccccgac gatggttatg tgactcaggt cacgctgcag cccggaacca
     7201 tggtctcgat agggccggtg atggccttta tcggccagcg gaccaaaccc ctgctggttg
     7261 tgaccgtcat gcagaattac gtcaacgtgg tgaaacccgg cgccaaagcc gagctggcga
     7321 cccccgctct acccggcaag atcctgcacg cccgggtgct ggcagtcgag cgggccaccg
     7381 gttccggcgc gctttacccc gaaggccgtc tgaagcgagc tttcgaaccg gcctttcccg
     7441 atcgcatgta cgtgatcctg aacctggatg acgagctcaa ggacaccatc ctgcccatcg
     7501 gcaccaacgg atggatctcc atccagggaa agcaatggtc ggagcttttc atcatccggc
     7561 gggtcatcat gcgctggtac acatggacca actatatctt cactggctac tgagtccgga
     7621 gaatagggtg aatacaagcg tcaggttcca gaagctggta tcgggtgtgg ccgtctttgg
     7681 actggtggtg ctgctttgcg cctgtgcagt gaagaagccg cctctaacga cgcaggtggt
     7741 gcacgattcg cttccgccca ccaccacgat tcccgatcag tggacgagca cgcctgtaaa
     7801 tcccgctccg gtcgaaacgg gatggctgaa aagctttcat gatccccaga tggaagccat
     7861 cgtcgccgag gcgctcagga acaacctcga tctccaggcg gccgcgaccc gggtacaggt
     7921 ggccgccaac gtggtgaccg aagtacacgc acagttgatg ccccagatcg gtatcgcggg
     7981 agaagccaag tacctcggcc gcttccacga aaaaaatgcc acagggaaag cgatcgggaa
     8041 ctttaacgcc tccagcctgc tgggcggcgt ctcatgggaa ctggacgtgt gggccaagat
     8101 ccgctcccag gttgcggcgg cgaaagaagg tcaagccgca accgggtcgg attatcagtg
     8161 ggcacggatg tcgctggcag ccatcacggc cagggtctgg tatctggcgg ggtacaccgg
     8221 tgtgctgcaa agctatgccc aacagaatgt cgcgattgac caaaagctgc tctacgtcac
     8281 catcgcgcaa tttcaagtgg gcaatgccga gcagcagcag gtggacttcg cgcaggcaca
     8341 gcttgcgcaa tcacaagccc aactggcgca ggtcagcaat gcctacctcc aaatcgtccg
     8401 cggactggaa gtactgctgg gccgctatcc cgcaggcgaa ctgaaaaccg ctagcgctct
     8461 ggccgctttg ccgcctccgg cccctgctgg tttgccgtca caattgctcg agcgccgacc
     8521 ggatgtgctg gcggcgcagc atgacttcaa cgcggctttt catctggtgc agaccgccaa
     8581 ggccgcgcgg ttgccatcgt tcgccctgac tggcgccggc ggttatctca ccaatgagat
     8641 ttttcaggac ctcaagtttc gtccctgggt ttggacggtc gcgggcaaca tgattgctcc
     8701 gctctatacc ggcggctact tgcaggcgca ggtgaagatc tccaacgaaa accagaaggc
     8761 cgcgctcgcc ctttacggcc aggcattact gcaggccttc gacgacgtcg aggtcacatt
     8821 caccaacgag cgctacttga aggaccagga gaaggatgca actgaggcgt tgaaaagcac
     8881 cgaggatgcg ctcaacctgg ccaaagtgaa gtacgaagtc ggtcagaccg atctctcgcc
     8941 ggtgctgcaa ctggaaagcg tggtgttagc gactcagacg gcggacacct accttcagta
     9001 tgaacttatc gccaatcgca tcgaccttta tcttgcattg ggaggaccgt tctaaatagt
     9061 ggagtgttca ccagagcagc gtgccattcg gagcggtgtt agtagttctc aaaatgtgtt
     9121 gcaatcgtgg cggatcgctc cggtctccga tgtttcgagt aatgcatgca ttgctcctcc
     9181 cgcgctgtca tagcacgtcg ctagaaataa tggaactcgg attccgtctc tgtgctgtcc
     9241 tagttggact tcgcgcatcg acttggctga cctgcatccc gaaatttgat ccagcgtaac
     9301 tgagagtttt ccggttaaaa tgccttgctc ggcggagcag ggcgaggcag gagaacttag
     9361 ccttgaagaa gagccgattc gccgcagaac agatcgtagg aattttgcag gagtccgagg
     9421 ccggcctggg cacggcgaag ttgtgccgga agcacgccgc cagccaacag actttttacc
     9481 gttggaagtc gaggtacggc ggactgcagg tgagcgaagc gcagcggctg aagcagttgg
     9541 aagccgagaa ccacaagctg aagcatctgg tcgccgatct gatgctggac aagcaggctt
     9601 tgccttgggt tccagattcg cggattgtac ggctgtacat ttctgtcact ttggggatta
     9661 tgattacgag ctttgaaaga gcaaagctta cgcgggcagg tcctccgacg tcgggaaacc
     9721 gtgctgctcg cggccgacct cgccatcgta cttcttggtc accttgtata gttcatagcg
     9781 cccgtcggag gcaaagaact tgcactgttc ccggagcgcc tgcatctgtt gctctggcat
     9841 tggctggaat ccacgggcca acgccaggtt ttgctccagc acctccatcg aatccacgcc
     9901 gctgatggtg gaggagacgg gcaggctcat ggcgtagcgc aacgcttgct ccggagttgc
     9961 cgcgccatgg atgatgatct cgccactgcc gcccatactc ttcatcccca ggacgcccat
    10021 gccgcgctgc ttcgccaccg gaagcacact agtttcaaag cttcgaaacg tcgcatccag
    10081 acaattcagc ggcatctgca ccgtatcaaa gggaaaatca tgtgaaagca ttttgaggtg
    10141 gatgctggga tccttatggc cggtgaaccc aatggcccgc actttgccct gttgtttggc
    10201 ttcgaagagg gcatcggccg caccgcccgg acgaaagatc aggtcgggat cgttttcgta
    10261 gatcatttca tggacctgcc acaggtcgag ataatcggtt tgcagtcgcc gcagcgactg
    10321 ctccagttgt tggatagcga tatccttgcc gcggccatgg gtgcagacct tactcatgac
    10381 gatggcctgt tggcgcttgc ctttcagcgc aattcccaca acctcttcgc tgtggccgtt
    10441 gtggtattcc cacgcattgt ccaaaaagtt gactccggcg tccatggcgc aggcaacaat
    10501 gtcatttacc tcctgctggt ctttcgccga gcccagatga taaccaccca gtgccatggc
    10561 ggagactcgg atacccgtgc tgccaagtgg ccgatgcggc accagtcctg cggcgttctc
    10621 atgctcttgg tttttctcca ctcttttctt gctccagatc ttccggtcag cggttgttgt
    10681 tactatttct ctatcgaccc gatccgatct tcgaaaacgg atgccctgta ttcttggtca
    10741 cctgtatcat ctccttcatc tccacctgct gcacatcgac cggaaattcg tgtgccaacc
    10801 gcgcctcggg gttgtcgaac ttcagcgaca tcttgtacag ctcgaagcgc ccgtcggcgg
    10861 cctgtacctt cacccgctcg cgcagcgcct gcatctccgg ctgcgacatc ggctgaaacc
    10921 cttgcgcgat cgccagattc tgatgcagca cgtcgaggct ctccatcccc gtgatggtcg
    10981 tcgccaccgg caggctcatg gcgtagcgga gcgcttcccg caccgtaatc actccgcgac
    11041 tcacgggctc gccgtgccca ctgatcggct tcatgcccag cgccgcaata ccgcgcttgt
    11101 taagttcggg caagacgtgg gtttcgaagc tgtgaaactg cgaatcgaac acgttcagcg
    11161 gcatttgcac cgcgtcaaac ggaaagccgg tggccagcat tttcaagtga atgtctgggt
    11221 gcttgtggcc ggtgaacccg acaaaccgca ccttgccctg ctctttggcc ttatgtaggg
    11281 cctcggcggc gccgttgggc cggatgaaca gctccggatc gttgtcgaaa cagacaccgt
    11341 gcacctgcca gagatcgagg tggtcggtct ggtaccggtt cagcgactgc tccagcatct
    11401 gcaacgcgag gtcgccgtcc cggccgtggg tacagacctt ggtcatcagg aagaccctgt
    11461 cgcggcggcc cttcaggccc ttgcccatcc aaatctccga cttgccgagg tgatactccc
    11521 agcagttgtc gaaaaaagtg atgcctccgt cgacggcctc gtgcaccatg cgcaccgccg
    11581 tcgcttcgtc gggcgcatcc ccgagatggt ggccgcccat gcctagcgcg gagatcctgg
    11641 cgtcggtatt gccgaatcgg cgaagaggaa taccggaact ggcggaatct tgctccatag
    11701 atcaacaatc ctaggtcatt ggctccaagg gatgccgaat tgctgcaggc ggactagaac
    11761 aggaagacca ccgccgatag attcgttcag cacggagtcg aaacgggaca ctatttcctc
    11821 cctatcagtg actgcttttc tgctgggatc ttcttgattt cgccctcgtc gagattcaag
    11881 tgcgaagtaa ccatttccgg agggagtcgc ctgatccagt tattcaggga gaagtccaca
    11941 aactcgctcg ccttgaacat ttccaggaat accagatctg tatcgcctgt gttctcgacg
    12001 caatggcccg cgacagctgg aatgtagcca acatcgttgg cgtggaagtc catcgtacgc
    12061 gcatttccca ctggggagaa aacagtcatg cgaccttttc cagcgatata gaactgccac
    12121 tcggaggcgt tcggatgcca atgcagctcc cgtatgccgc caggcttgac ggtgacgagt
    12181 cccgcggcga tattccgtgc gactgggaag ttccgggaat cgaccacccg agcctctcct
    12241 ccagccgttt ttttggtggg aggcattgat tgcatcttga atgtgtactg gatcggggac
    12301 tgcactttct ttccaccaac agcggcgcga tcttggtcga gggaacgggg aagctccgcg
    12361 gggaagatgt agagttcgtc ggtcggcaac ttcgtgagaa cgctctcgtc cagcccaaag
    12421 ttcttcgaca gtaccgatgg cggagtatgc gccacccact ctgaaatgag gaatgtattg
    12481 tcctctgaaa acataccttg atcaaagacc agtagaaact cgcaaccatc cggtccgagt
    12541 ccctggatgg aatgcggaaa cccggcagg
//
LOCUS       DASRKK010000231         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_16925, whole genome shotgun sequence.
ACCESSION   DASRKK010000231 DASRKK010000000
VERSION     DASRKK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16925"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="recO"
                     /locus_tag="VFC15_04980"
     CDS             complement(<1..629)
                     /gene="recO"
                     /locus_tag="VFC15_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZM09545.1"
                     /translation="MSLKQSEAIVLRTYPLREADLLVTLFTRAEGKVKGIARSAKKSK
                     RRFGGALEPLTWVRAYYEDREGQELARLDSCDILESPLSATVDYPRVVALEHVAETLD
                     ELLPDREANDDIFRLAVSVLHQLRSGSIWMPLTYFQLWMVRLVGFLPELSECSACGQA
                     LNGSRAFYHALADGLMCVQHKRLASSELTADSRLLAAQMFRAPVDTFAE"
     gene            complement(724..1965)
                     /locus_tag="VFC15_04985"
     CDS             complement(724..1965)
                     /locus_tag="VFC15_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZM09546.1"
                     /translation="MGITRRAFMKGGAMAVVGTTAIPSFLTRAAWGAVNTGASNKRLV
                     VIFQRGAADGLNIVVPHAEPAYYAMRPSINIPRQQVIDLNGFFGLHPAMAPLKPLWDQ
                     RHLAVVHAAGSPDTTRSHFDAQDYMESGTPGVKDTSDGWLNRTLQSEPAVADSPFRAI
                     ALGTSLPRILSGKASAVAVSNVNGFGIAAGNPAAAPLGNTFEAMYAQSVDTVLHGTAT
                     ETFEAVKMLKSADPQKYTPAPGANYPRGPFGNALRQTAQLIKSNLGVQIAFTDIGGWD
                     SHVNEGAVEGQLANVLRDFAQSIAAFWVDLGPLAEETLIVTMSEFGRTARENGNRGTD
                     HGHANVMFILGGPVRGGRVYGRWPGLDPSQLNEGRDLVLTTDFRRVLGEAVSRHLGNA
                     HLNSVFPGFDNQPGKFLQYLG"
     gene            complement(2033..4075)
                     /locus_tag="VFC15_04990"
     CDS             complement(2033..4075)
                     /locus_tag="VFC15_04990"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZM09547.1"
                     /translation="MSQTQPKTGKGKLRWVTLAVLLCAAAASLWAARKPKSADPLNEQ
                     KRALHALDRLTFGPRPGDVQSVAAMGVDKWIEQQLHPEKIDDSAMQARLASYRTLQMN
                     SREMLLNFPPDPVINLMQAGKLPMPGDPYRHAIYVAAIDRMQQRQAQKQNVPAANAGT
                     GDQKNVPSPVSLQRQQDRHDAHSIVDDLVLLPPDQRMQHILNLPVTQQRDLTQGLPLE
                     KRQALLAGFSPEQRETVLALNNPQGVVNSEVQSAKLLRAVYSDRQLEEVLTDFWFNHF
                     NVFIGKGADNYLVTSYERDVIRPHVLGKFKDLLVATAQSPAMLFYLDNWQSEGPDSDA
                     ARGLPDRAQAARMNGPFGPRRSPGYPRPPQQQPNAQNKQPKRSGGLNENYARELMELH
                     TLGVNGGYTQQDVTQVARVFTGWTIDQPFQGGGFAFKPRLHEPGSKTVLGHTIKDGGE
                     KEGMQVLEILARNPSTARFVSTELAQRFVSDNPPQSLIDAMSKTFLKTDGDIREVLRT
                     MFRSPEFWAPEAYRARVKTPFEFVVSSLRATQADIADPQALLGTLNRMGMPLYGAQPP
                     TGYSTKADVWVNSAALLDRMNFGLALSTNRLPGTSFNLAQLTNKAATSDADPYQVQLD
                     LEQTLLSGDVSKQTHQTIEAKIVSPGVVAQFANPNRPMNVNVIAGLLLGSPEFQRK"
     gene            complement(4532..>5098)
                     /gene="rho"
                     /locus_tag="VFC15_04995"
     CDS             complement(4532..>5098)
                     /gene="rho"
                     /locus_tag="VFC15_04995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZM09548.1"
                     /translation="FDEPAARHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYN
                     TIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIETGSRMDDVIFE
                     EFKGTGNMEIILDRKLVDKRTFPAIDIQRSGTRKEELLIPKEDLARVWVLRKVLNPLS
                     PVEAMELLIDKLGKTRSNAEFLSNMSAL"
BASE COUNT          997 a         1519 c         1533 g         1049 t
ORIGIN      
        1 tcctccgcaa acgtgtctac cggagcgcga aacatctggg ctgccagcag gcgcgaatcg
       61 gcagtgagtt ccgacgacgc cagccgcttg tgctgcacgc acatcaggcc gtcggcaagc
      121 gcatgataaa acgcgcgact gccattgagg gcctgtccac aagcgctgca ctcgctcagc
      181 tccggcaaaa atccaaccag ccgaaccatc caaagctgaa agtaggtcag cggcatccaa
      241 atcgaacctg agcgcaactg atgcaacacg gaaaccgcca gccgaaagat gtcgtcgttc
      301 gcttcccggt ccggcagcaa ctcatccagg gtttccgcga catgttccag cgccacaact
      361 cgcggataat ccaccgttgc cgacagcggc gactccagga tgtcgcacga atcgagccgc
      421 gccagttcct gcccctcgcg gtcttcgtaa tacgcccgca cccaggttag cggctccagc
      481 gctccgccaa aacgccgttt cgatttcttg gccgaccgcg cgatgccctt gaccttaccc
      541 tcggcccttg tgaacagcgt caccagcaag tcagcctcgc gcagcggata ggttcgcaac
      601 acaatcgctt ccgactgctt cagtgacatg gaggttcaat cgactgccgt caaacttcga
      661 ttctactggc agcgattgcg atgtgcaaag cgtggggaga ggtggggccc tcgattgatt
      721 cgttcatccc agatattgca ggaacttgcc tggctgattg tcgaagcccg gaaacacgga
      781 gttcaaatgt gcgttgccga gatggcgcga gaccgcttcg cccagcacgc ggcgaaagtc
      841 ggtggtgagc accagatcgc ggccttcgtt gagctgggat ggatcgaggc cgggccagcg
      901 tccgtaaact cgtccgccgc gcaccgggcc gccgaggatg aacatcacat tggcgtggcc
      961 gtgatcggta ccgcgattgc cgttttcgcg cgccgtgcgt ccgaactccg acatggtgac
     1021 gatcagcgtc tcctccgcca gcggacccag atcaacccag aatgcagcta tggattgcgc
     1081 gaagtcgcgc agaacgttgg cgagctggcc ttccacggca ccttcattca catggctgtc
     1141 ccagccaccg atgtcggtga acgcaatctg tacgccgagg ttcgacttaa tgagctgtgc
     1201 ggtctggcgc aaagcgttgc cgaagggtcc gcgcgggtaa tttgcgcccg gcgccggcgt
     1261 gtacttctgg ggatcggcgg acttcagcat tttgaccgcc tcgaaggtct cggtggcagt
     1321 gccgtgaagc acggtatcca ccgattgtgc gtacattgcc tcgaaggtgt tacccagcgg
     1381 ggcggcggcg ggattgcctg cagcgatgcc gaatccgttc acgttgctga ctgcgacggc
     1441 cgacgctttg cccgaaagaa ttcgtggcag cgaagtgccg agggcgatcg cgcggaacgg
     1501 cgagtcggcg acagcaggct cggattgcaa cgtacggttg agccagccat cgcttgtgtc
     1561 cttcacgccg ggagtgcccg actccatgta gtcctgtgcg tcgaaatgcg aacgcgtagt
     1621 gtcgggcgag cctgcggcat gaacgacggc gagatggcgc tggtcccaaa gcggtttgag
     1681 aggcgccatc gcaggatgca ggccgaagaa gccattgaga tcgatcacct gctgccgcgg
     1741 aatattgatg ctgggccgca tcgcatagta ggcaggctca gcgtgaggca ccacgatgtt
     1801 gaggccgtcg gctgcgccgc gctggaagat gactaccagc cgtttgttgc ttgcgccggt
     1861 gttgactgcg ccccacgccg ctcgcgtgag aaatgaagga atcgcggtgg tgccgaccac
     1921 cgccatcgct ccgcccttca tgaacgctcg ccttgtgatt cccatgattc gctattcgcc
     1981 tttcgctatt cgcctttcgc cacacccgaa gggcttgtcc agtcgagatg catcactttc
     2041 gttggaactc cggcgagccc aacaataatc ccgcgatgac gttcacattc atcggacgat
     2101 tcgggttggc gaattgcgcc accaccccgg gactgacaat cttcgcttca atggtctggt
     2161 gcgtctgttt cgaaacatcc ccagagagca gcgtttgctc cagatcgagc tgcacctgat
     2221 acggatcggc atcggaggtg gcggccttat tcgtcagttg cgcaagattg aaactggtgc
     2281 cgggcaagcg attggtcgaa agcgccagac cgaagttcat ccggtcgagc agggctgccg
     2341 agttcaccca aacatctgcc ttggtcgaat agccggtcgg aggctgcgca ccgtacagcg
     2401 gcatacccat tctgttcagc gtccccagta gcgcctgcgg gtcagcgatg tcggcctgcg
     2461 tggcgcgcaa cgaggagacg acgaactcaa acggcgtctt cacccgggcg cgataagcct
     2521 cgggcgccca gaactcaggc gaacggaaca tggtgcgcag gacctcgcga atgtcgccgt
     2581 cggtcttcag gaaggttttc gacatcgcgt caattagcga ttgcggcgga ttgtcggaaa
     2641 caaatcgctg ggccagttca gtcgaaacga accgcgcggt agaaggattg cgggcgagga
     2701 tttcgagcac ctgcatgccc tctttctcgc cgccatcctt aatggtgtgg cccagcacgg
     2761 tcttgctgcc cggttcgtgg agtcgtggct tgaacgcgaa accgccgccc tggaacggct
     2821 ggtcgatggt ccatccggtg aagacgcgcg ccacttgagt cacgtcctgc tgggtgtatc
     2881 cgccattgac gccgagagta tgcagctcca tcaattcgcg ggcatagttt tcattcaacc
     2941 caccgctgcg tttcggctgc ttattctgcg cattgggttg ctgctgtggc gggcgcgggt
     3001 acccgggcga gcgccgcggc ccgaacggtc cgttcattct ggccgcttga gcacgatcag
     3061 gcaggccgcg ggccgcatcg gaatcaggcc cttcactctg ccagttgtcc aggtaaaata
     3121 acatcgctgg actctgagcg gtcgccacca acaagtcttt gaacttgccc agcacgtgcg
     3181 gacgaatgac atcgcgctca taagacgtga ccagataatt gtcagcgccc ttgccaatga
     3241 aaacgttgaa atggttgaac cagaaatcgg tgagcacttc ttccagttgg cgatcactgt
     3301 aaactgcgcg cagcagcttg gccgattgca cttcactgtt gacgacgccc tgcggattgt
     3361 tcagcgccag cacggtttcc cgctgctccg gcgaaaaacc ggcaagcagt gcctggcgct
     3421 tttcaagggg aaggccttgt gtcaggtcgc gctgctgcgt cacaggcaag ttgaggatat
     3481 gctgcatgcg ttgatcagga ggcagcagaa ccaagtcatc tacgatgctg tgggcgtcat
     3541 gccggtcttg ctgccgctgt agcgagaccg ggcttggcac attcttctgg tcaccagtgc
     3601 cggcattcgc ggcgggcacg ttctgcttct gtgcctgcct ctgctgcatg cggtcgattg
     3661 cggctacgta gatggcgtgg cgataaggat cgcccggcat cggtagcttt ccagcctgca
     3721 tgaggttaat gacgggatcg ggcggaaagt tgagcagcat ctcgcgcgag ttcatttgca
     3781 atgtgcgata gctggcgagt cgcgcttgca tagcactatc gtcgatcttc tcgggatgaa
     3841 gttgctgctc gatccacttg tccacgccca tcgcggcgac ggattgaacg tcgccgggac
     3901 gtggcccgaa ggtgaggcga tccagcgcat gcagcgcgcg cttctgctca ttcagaggat
     3961 cggcgctctt tggtttcctg gcggcccaaa gacttgccgc tgcggcgcac aacagcaccg
     4021 ccaacgtaac ccaacgcagt ttgcctttac ctgttttggg ttgtgtttgg ctcattcagc
     4081 gtcgtgcctc tggcaaagct tcagtgttta taaacctgcg gtgagcggga agttgctgga
     4141 gggccggcgg acgtccaagc ggggcaggtt gtgcaggcct cacccgcgcg agcgtggttg
     4201 cttatgataa ggctgtctcg tacacaacaa aaaggcgcgg aaaccctcag ccttccgcgc
     4261 ccatcacctc gatccaaatt attattgttg agctgctcaa ctatcgtcgg cgtaagaatg
     4321 agagctgaag agggtgagaa gtgtgccgtc cctgaaggga ctcgtatcta ttttccacgt
     4381 tacccagcgc tgaagcgctg ggctaagctc tttcgcccca ttcggggctc gattttgcgc
     4441 actcgttcca ccgtgccaac tcaggacgag gtctcacaca cgcactgccg gacaatgact
     4501 gagagctgac ggctgaaagc tgacagcttc cctacagcgc gctcatgttc gacaaaaact
     4561 cggcattcga acgcgtcttg cccagtttgt cgatcagcag ttccatggct tccaccggcg
     4621 acaaggggtt caatacctta cgcaacaccc agacgcgcgc caggtcttcc ttgggaatca
     4681 gcagctcttc cttacgcgta ccggagcgct gaatgtcgat ggccgggaag gtgcgcttat
     4741 cgaccagctt gcggtcgaga atgatttcca tgttgccggt acccttgaac tcttcgaaga
     4801 tgacgtcgtc catgcgcgac cccgtctcga tgagagcggt cgcgatgatg gtcagtgaac
     4861 cgccttcttc gatgttgcgg gcggcgccga agaaccgctt cggacgctgc aacgcattgg
     4921 aatcgacgcc gcccgagagc actttgccgg agggcggaac aatcgtgttg taggcgcgag
     4981 ccagacgggt aatggagtcg agcaggatga ccacatcgcg tttgtgctcg accagacgct
     5041 tggccttctc gatgaccatc tcggccacct gaacgtggcg tgccgccggc tcgtcaaa
//
LOCUS       DASRKK010000232         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1091999, whole genome shotgun sequence.
ACCESSION   DASRKK010000232 DASRKK010000000
VERSION     DASRKK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091999"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFC15_05000"
     CDS             complement(<1..212)
                     /locus_tag="VFC15_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09549.1"
                     /translation="MPIPLRGLRTIRTLAGKVELVSVPHRALLQVACLELEKARRTTE
                     RSSAFRRVAELDARIRDIDAEQASLL"
     gene            complement(215..1285)
                     /gene="gvpN"
                     /locus_tag="VFC15_05005"
     CDS             complement(215..1285)
                     /gene="gvpN"
                     /locus_tag="VFC15_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpN"
                     /protein_id="HZM09550.1"
                     /translation="MPALELMPDPIAEPITREESPANGDGSKTIEDVELSVPADDINV
                     VPEPSDSFVSTPYIESLTARALTYLDVGYALHFAGPAGTGKTTLALHVARKRGRPVVL
                     IHGDDEYASSDLVGKDSGYRKSKLVDNYIHSVVRTEESLNRVWEDQRLTTACIKGFTL
                     VYDEFNRSRPEANNVLLSVLEGKILNLPNLRRSGEGNLHVHPEFRAILTSNPEEYAGV
                     HKTQDALLDRLITMSLGHLDRDTEIAITMAKSGLPQPDAEIIVDIIRELRGVGVNNHR
                     PTIRAGIAIARILAHHNGSAQVGDSVFETICHDVLNTDTAKITHQGQSLMAEKVKETI
                     RTVCGDRPQGSKNRRKPERGRR"
     gene            complement(1780..2073)
                     /locus_tag="VFC15_05010"
     CDS             complement(1780..2073)
                     /locus_tag="VFC15_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09551.1"
                     /translation="MGKLTKDMTRLCDEIQTLRADRQELKKELAEKTKARQVEILQTC
                     AAFTDARVWKAEDARNNRQNFLDNLKQVVADQVKDTRDDLAMARRSWGKPRQA"
     gene            complement(2184..2402)
                     /gene="gvpA"
                     /locus_tag="VFC15_05015"
     CDS             complement(2184..2402)
                     /gene="gvpA"
                     /locus_tag="VFC15_05015"
                     /inference="COORDINATES: protein motif:HMM:NF006874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HZM09552.1"
                     /translation="MAKVQKATDSSSLAEVVDRILDKGIVIDVWAKVSLVGIELLSIE
                     ARVVIASVETYLKYAEAIGLTASAAAPA"
     gene            complement(2902..3621)
                     /locus_tag="VFC15_05020"
     CDS             complement(2902..3621)
                     /locus_tag="VFC15_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZM09553.1"
                     /translation="MRYLLYCIFRGAPQPELEAPTGVEGKPVLVIDHGGLGAAISELS
                     EPDPLPDVAAVLAYESVVESFFSRRTIIPMRYGCTVRDRSELAAVLDEHRQQCGALLD
                     GLEGLAEMGIQVLADGPEAGPGVDSAVVSPSHFHDSNRSGISYLLAKKQYYGSADGMA
                     ERQNELVKTLCHPLSGLFVRRKVELPSSGARQLSLYFLVPRNSIESFRAASQQCLKDG
                     LAKPLVSGPWPPYNFVEFSKT"
     gene            complement(3618..>4011)
                     /locus_tag="VFC15_05025"
     CDS             complement(3618..>4011)
                     /locus_tag="VFC15_05025"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZM09554.1"
                     /translation="LLSARLTAEQKQLSALPPGKRHFAEQRIGRDIERDLSIWLDRTC
                     RTIAADLTSYTSDFRECALSPHAHSESGAEEVLNWAFLLPESAIGPLQEHLKQANLDC
                     QSQGLLLQLSGPWPPFRFVPALPSTGAP"
BASE COUNT          792 a         1162 c         1146 g          911 t
ORIGIN      
        1 tggaggaggc tagcctgctc cgcgtcgatg tccctgatcc gggcatcgag ctctgccact
       61 cggcgaaacg cgctgctccg ctcggtagtg cgccgggcct tctcgagctc caggcaagcg
      121 acctgcaaga gcgcccgatg gggaaccgaa accagctcca ccttccctgc cagtgtgcga
      181 atggtcctca gacctcgaag tgggatgggc acgtttacct ccttccacgt tccggtttgc
      241 ggcggttctt gctcccttga ggccggtcgc cgcacacggt gcgaatggtt tctttcacct
      301 tttccgccat cagggattgg ccctgatggg taatctttgc ggtgtcggtg ttgagcacgt
      361 cgtggcagat agtctcaaag actgaatcgc caacttgcgc ggagccgttg tggtgggcga
      421 ggatgcgggc gatggcgatc ccggcccgga tggtgggacg atggttgttg acccccactc
      481 cacgaagctc tcggatgata tcgacgatta tttccgcatc cggctgggga agtccggact
      541 tcgccatggt gatggcaatc tcagtgtcgc gatccaagtg tcccaggctc atcgtaatca
      601 ggcggtccag cagtgcgtcc tgggtcttat gtacgccggc atactcttcc gggttcgagg
      661 taagaattgc gcgaaactca ggatgaacgt gtagattgcc ctcgccagac ctgcgcagat
      721 tgggcaagtt gagaatcttg ccctccagca cgctgaggag aacattattg gcctcgggcc
      781 gggaccgatt gaattcgtcg tacaccaggg taaagccctt gatacaggcg gttgtcagcc
      841 tctggtcttc ccaaacccgg ttcaagctct cttccgtcct tacgaccgag tgaatgtaat
      901 tgtccaccag cttggatttg cgataaccgg aatccttgcc caccaggtcg gaactggcat
      961 actcgtcatc gccatggata aggaccacgg gccggccccg cttcctggca acgtggagcg
     1021 ccagggttgt ttttcccgtt cccgccggcc ccgcaaagtg cagcgcgtat cccacatcca
     1081 agtaagtgag cgcgcgggcg gtgagactct cgatgtaggg agttgagacg aacgaatcac
     1141 tgggttccgg aacaacgttg atgtcgtcag ccggtacgct taactcgaca tcttcgatgg
     1201 tcttggaacc gtcgccattg gctggcgact cctctctggt gatgggctcg gcgatgggat
     1261 ctggcatgag ctcaagggct ggcacgatga tttggactcc ttccaattag ctttcactgc
     1321 gcgtgggcat ggttccccct ggttcgtctt ccgtcgcggg tgttcctcgc accacgcatg
     1381 agtttctgct ttctggtcct cggtttattc gaacggcggc gttgggcgta ctcgaaccca
     1441 ggctggccaa agcctcgcgg acgccaccga ggtcctcccg ggtttttttc tgcaagtcgg
     1501 agaccttttc cttaaggtca ctcagaaaaa ccgttcggtc ggctttccgc ggggcgattt
     1561 cggcaaagcc gttcacgaat tgagccaaca tttggcacac attgcgcttg cggtccctgg
     1621 tctcacgccg cattccggag agaaatcgct cacgttcctg tcgcaagcta aggatctcgt
     1681 tgcgaactcg agtcatgcct tcagaaagag ggtgcaccct cagcctcctt tgcttcccct
     1741 atcgctacta ccggcagggg ttcggcacag ccaggaccgt catgcttgcc gcggtttgcc
     1801 ccaggagcgg cgggccatcg ccagatcgtc ccgggtgtct tttacctgat cggccactac
     1861 ctgtttcagg ttatccagga agttctgccg attattgcgt gcgtcttcag ccttccaaac
     1921 tcgcgcgtcc gtaaaagccg cgcatgtttg gaggatctcc acctggcgtg ccttggtttt
     1981 ctccgcgagc tctttcttaa gctcctgacg gtcggcccgc agcgtctgga tttcgtcgca
     2041 cagccgggtc atgtctttcg tcaatttacc cactggtatc ctcctttggt cgtttctacc
     2101 ctggcagttt tggtattcag ccaagccaag ggggccaaac tcgccccccc gacttggttg
     2161 tggttatgcc ccctaaccat gccctaggcg ggagccgcag cgctcgccgt cagcccgatg
     2221 gcttccgcat acttcaggta ggtttcaacg gaagcgatca ctacccgggc ctcgattgac
     2281 agcagttcga tcccgaccag cgaaactttt gcccaaacgt cgatcacgat acctttgtca
     2341 agaattcgat cgaccacttc cgctaagctg gaagagtcgg tggctttctg aactttcgcc
     2401 atactatttc tcctttactc agatagaacg tcttgtctta cgtaataaaa gacagtgcaa
     2461 gccaatggcc ctacgatgac aatgcgagtg gatctccgac ttcttcagag ccgaactagt
     2521 ccgggcagag cggttgctat tcttatgaat aagatccggg caagtccaaa gaaataatcc
     2581 agctcaaatt gcagcgagag tgccgcgccc ctcagcaact aaggagttca ttccatggag
     2641 agcgtgcctt acagtcaggc atctgttccc cactgaagct agagcagtac cagagtggct
     2701 gtagcttagg tagtgcgggc cttcaagccc gcgttaaggt ctgcacaatt tctggcctta
     2761 gccgctgagg tcgacgacct cagcggctaa ggcgggtttc tttgtgcaac tgcacgcggg
     2821 cctgaaggcc cacactaccc gtaccagcgg atacaccaat cctggaaccg ctctaaggac
     2881 catattgttt atgagattcc gttaagtttt tgagaactca acgaagttgt acggtggcca
     2941 aggtccgctc acaagtggtt ttgcgagccc gtccttaaga cattgctggc tcgccgcacg
     3001 aaaggattcg atggaattgc gtggtacgag gaaatagaga gacagttggc gcgcaccact
     3061 ggaaggcaac tccaccttgc ggcgcacaaa caggccggac agcgggtggc aaagtgtctt
     3121 cacgagttcg ttttgccgtt ccgccattcc atccgcactg ccgtaatact gcttcttggc
     3181 caatagataa gaaataccgg atcgatttga atcgtgaaag tgggatgggg agactactgc
     3241 ggagtcaact ccggggccgg cttcggggcc gtcggccaga acttggatgc ccatctcggc
     3301 caacccttcg agcccatcga gcagcgcgcc gcactgctga cgatgctcgt ccaacacagc
     3361 ggcgagctcc gatctgtctc gaaccgtgca cccgtatcgc atgggaatta tggtccgtcg
     3421 gctgaaaaag gattccacga cactctcata ggcgaggact gccgcgacat cgggaagcgg
     3481 gtccggctcg ctaagctcag aaatggcagc gcctaggccg ccatgatcga ttacaagtac
     3541 cggttttccc tcgacgccgg tgggggcttc gagttccggt tgcggagcgc cgcgaaagat
     3601 gcagtagagc aagtacctca tggggcgccg gtcgagggca aagccgggac aaaacgaaat
     3661 ggcggccacg ggccggaaag ttggagcaac aatccctgcg actggcagtc caggtttgcc
     3721 tgcttcaagt gctcttgaag tggaccgatc gcggattcgg gcagcaggaa ggcccaattc
     3781 agcacctcct cggctccgct ttctgaatgc gcgtgtggcg acaacgcaca ttcgcggaaa
     3841 tccgaagtat agctggtgag atcggccgcg atggtgcggc acgtccggtc aagccagata
     3901 ctcaagtctc tttcgatgtc tctgccgatg cgctgttcag cgaagtggcg cttccctgga
     3961 ggcaacgcgg agagctgttt ctgttctgct gtcagccgtg cggacagaag g
//
LOCUS       DASRKK010000233         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_896332, whole genome shotgun sequence.
ACCESSION   DASRKK010000233 DASRKK010000000
VERSION     DASRKK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896332"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1752
                     /locus_tag="VFC15_05030"
     CDS             346..1752
                     /locus_tag="VFC15_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HZM09555.1"
                     /translation="MRVRYQRGYLRLGHRKTGPDCWEFLWWDIELTGHRVRRKAVIGT
                     VQQYPNIGEAWQASNGLRVFINEARNRQQEQLVTVSDLVDHYTRTELTDDPSDGGKSY
                     ATRTVYKNFLARWVRPAWGSLNIRAVRTTAVEQWLRQLTRADGGPLAPSTKAKIRNVM
                     SVLFNHAIRHEWLEQGKNPILLVRQGAKRQSIPVYLEPGELRSLLPQLDRCFRVMVLL
                     DAATGLRRSELLALKCEDIDFERLQINVRRSIYLNVVGNCKTEASRKPVPLDLTLASE
                     LWSWKQDSAYGKPNDWVFASPRSRGRNPYWPDILLSRVVRPAAARAGIRKHIGWHTFR
                     HSFSTMLMANGENVKVVQELMRHANCRCTLEIYSQARLQAKRDAQHRVVEMIISRERK
                     ANDAPLPNELLRKLRLLRDENRPITLLRSFGVLPFWKEISGVTVAHPWLIYAELMRSP
                     DSRAHEAAEQLRAEFIHD"
     gene            1745..2152
                     /locus_tag="VFC15_05035"
     CDS             1745..2152
                     /locus_tag="VFC15_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09556.1"
                     /translation="MIDFTEAELTDLIQLQDICNELKTQLVIIGAMAYKFHFPEIERY
                     TADIDLTVALDLDDFAELEMRLSKFGWNRDAKREHRWTSPRATLIDLLPARAKLRAAK
                     KLIWLESQLSMSLVGVFTLLREELEGFSSNKLV"
     gene            complement(2105..>2397)
                     /locus_tag="VFC15_05040"
     CDS             complement(2105..>2397)
                     /locus_tag="VFC15_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HZM09557.1"
                     /translation="ATSFSLIRSISVPLGLAEAGKPNIAGTIWRSISDISAKRYYFES
                     AYSPSIFWVDLDKLKLSAGSKPSRLDLTSRPIYAGEVSDKFVATEPFKFLSQ"
BASE COUNT          561 a          645 c          688 g          503 t
ORIGIN      
        1 atcctccccc ggttcggcgc gcttaatgga gccatttgga gcctcgcggg acgaggtgag
       61 agaagctaag tgattgaaag aaatggtagg cacgaggaga ctcgaactcc tgacctctac
      121 cgtgtcaagg cttttcaaca acttacaagt gcgtggagac tgcctaagta cacgcaagtc
      181 gtacaagaca tcgctttttg tgggttggat tgtgggttga aaatttgggt acacaccaac
      241 gccacttgca agccaagcga catccgacca cacagtagga tcgccctcct gatgctcgca
      301 gtcataccct ttctcccgtg tacacatcat tcggaggagg ggaaaatgcg agtacgctac
      361 caacgaggat atcttcggct tggccatcgc aagacgggac cggactgctg ggaatttctt
      421 tggtgggaca tcgaacttac agggcacaga gtacgccgca aggcggtcat aggaaccgtt
      481 cagcaatacc cgaatattgg ggaggcttgg caagccagca acggcctccg ggtgttcatc
      541 aacgaggctc gaaaccggca gcaggaacaa ttggttacgg tctccgactt agtggatcat
      601 tacacgagga cagaacttac cgatgatcca tcggatggag ggaagtcata cgccacgagg
      661 acagtgtaca aaaactttct cgcgagatgg gtcaggcctg cgtggggcag cttgaacatc
      721 cgggccgtcc gcaccacggc tgtcgaacag tggcttcgtc aactgacgcg cgccgacggc
      781 ggtccgctcg ctccctcgac caaggcgaag atccgcaatg tgatgagcgt gctcttcaat
      841 cacgctatcc ggcatgagtg gctggagcaa ggcaagaatc ccatccttct cgtccgccag
      901 ggtgctaagc ggcaaagcat cccagtgtac ttggaacctg gagaattgag gtcgctgctt
      961 ccccaactcg atcgctgctt tcgtgtaatg gttcttctgg atgcggccac aggactgcga
     1021 cgcagcgaac tgcttgcact caagtgcgaa gacattgatt ttgaacgatt gcaaatcaat
     1081 gtgcggcgtt cgatctatct gaacgtcgtc ggcaattgca agaccgaggc ttctcgaaaa
     1141 cctgtgccgc ttgatctgac tctggcttca gagctctgga gctggaagca ggacagcgcg
     1201 tacggcaagc ctaatgactg ggtttttgcg agcccgcgca gtcggggcag aaacccgtac
     1261 tggccggaca tcctactttc tcgcgtggtg cgcccggcgg ctgcgcgtgc ggggattcgg
     1321 aagcatattg gctggcacac gtttcggcac agcttttcga ccatgctgat ggcgaacgga
     1381 gaaaacgtga aagtggtgca ggaactcatg cgtcatgcga actgccggtg cacactggag
     1441 atttattctc aggctcggct acaggcgaag cgcgacgcgc agcatcgggt agttgagatg
     1501 atcatctctc gggagcggaa agccaatgac gcgccattgc caaacgagct tcttcgcaaa
     1561 ctgcgcctcc tacgtgacga gaaccggccc ataactcttc tccgttcatt tggagtcctg
     1621 cccttttgga aagagatatc tggggtcacc gtcgctcacc cctggttgat ctacgcggag
     1681 ctgatgcgct cccctgattc cagggcgcac gaggccgccg aacaattacg ggccgagttc
     1741 atccatgatt gatttcaccg aagcggagct caccgatctc attcagctcc aagacatctg
     1801 caacgaactc aaaacgcagc tcgtcattat cggcgcgatg gcctacaagt tccactttcc
     1861 cgagatagaa cgctacaccg ctgacattga cttaaccgta gcgctcgacc tcgacgactt
     1921 tgcagaactt gaaatgaggc ttagtaaatt tggctggaac cgggatgcga agagagagca
     1981 ccgatggacg agcccgcgcg cgaccctgat cgaccttttg ccggcccggg caaaattacg
     2041 tgcagcaaag aagctcatct ggcttgagag ccaactctca atgagcctcg taggcgtctt
     2101 tacgttactg cgagaggaac ttgaagggtt cagtagcaac aaacttgtct gatacctcgc
     2161 ccgcatagat cgggcggctg gtgagatcga ggcggctggg tttcgagccg gccgagagct
     2221 taagtttatc gagatcgacc cagaagattg acgggctata ggcagattcg aagtagtatc
     2281 gcttggcgct aatatcggag atggaccgcc agatggtgcc ggcaatattc ggttttcctg
     2341 cttcggccag gccgagcggc accgagatgc tacggatcag cgaaaacgat gtggcga
//
LOCUS       DASRKK010000234        15570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_896539, whole genome shotgun sequence.
ACCESSION   DASRKK010000234 DASRKK010000000
VERSION     DASRKK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896539"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1177)
                     /locus_tag="VFC15_05045"
     CDS             complement(107..1177)
                     /locus_tag="VFC15_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZM09558.1"
                     /translation="MQRQIITVLALSCVLITAGYAQTDSPGTPLPPPTRAEKRAAVAS
                     RANSGASAANPSTVGMDQPVITLKGGCEPIGDIAPAKDCVSSITRAQFEKIINALQPD
                     MAAEAKRGFATNYGRLLVFFDAARAMHLENDPTVQQIIQFVSQQVLAEGLKRHYTEQF
                     AHPSDQQIQDYYNQNSSKYLEVTLQRIIVPKNPGSADKPALSEADAAVAAEKIRQRWV
                     AGEDPVKLQQSAFEAAGMTGASTPEINLGARRPGSLPVNQESVFQLKAGEISQTYSDP
                     AAAYIYKVVTVRQIPLSEVKDSIVKTLQQQQLQEKLDAIGKSATPVLNDEYFGPPPPA
                     AAPATGGRPGQGGPPQSGNPPQ"
     gene            1297..2718
                     /locus_tag="VFC15_05050"
     CDS             1297..2718
                     /locus_tag="VFC15_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HZM09559.1"
                     /translation="MPESVNIDARQSEKQSVALNSVFAAVAITSLKIVVGISTGSLGI
                     LSEAAHSGLDLVAAAITYLSVRVSDKPADADHQYGHGKVENFSAFIETGLLLLTCLWI
                     VYEATKRLFFHSVEIEPSVWAFVVMAISIMVDFWRSKRLKRIADKYDSQALLADALHF
                     GTDIWSSAVVILGLSLVMAGRTYSVPWLAKADPIAALVVACIVVYVSWRLARQTIDAL
                     LDGAPAGVRSKIIDAVSRVDGVLEIDRVRIRRGGSKYFADVSIAMSRSVTFQKSEQVA
                     NEVSSRIRELLPEADIVVNAVARASRGESLFDRIRAVATRNNLNVHDISVQDIHGALH
                     VEQHVELDERMPLKDAHDRVTRLESEMRRDIPEISSILTHIESEPATIETSDGVVDAP
                     SFERRIQLATGAFPEVVDAHDLMFKRVAGRLYLSLHCTMKDDLPLARVHDIQTALESR
                     FRQEVPDLFRVLIHPEPQTDNRR"
     gene            complement(2728..4326)
                     /locus_tag="VFC15_05055"
     CDS             complement(2728..4326)
                     /locus_tag="VFC15_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM09560.1"
                     /translation="MTLPMAPLRTQLYANPDGKFVHDVVLESCRQHGDKIAIVDMSCT
                     PPRRISYGEYAELVERTAHGFVVAGIRPGEMIGIFLPNCWEFGVAFHAATMVGAIPTT
                     MNPTYREREVHHQMETSDAVALISDGPLLEGIDLSGLPGLRNVFTIRTATPGGSEPMS
                     KLYSSTARVSLPTPEHDSRLTIATLPFSSGTTGLPKGVMLSHHNLVANVYQTLTSGET
                     GCIGESDVVLCFLPMYHIYGMTVGMNLCLMRGCTVVLMPRFDCEGSLRAIVDEGVTMA
                     LCVPPALLAYCHACDAGKFPREHGLRWVKCGAAPLAPDLARRFTEATGILIRQGYGMT
                     EASPVTHMGYLDPQFYNSASIGGPVAQTECRVLDENGNDVPQGELGELVMRGPQFMLG
                     YWKSPDATAAVMHDGWYWSGDVVRVDEQGLYFVVDRRKEMMKYKGFSIAPAEVEGVLL
                     EHPAVRDCGVVSRVDEASEEIPCAFVVLRESHLETEQTTDSIRDFVAERLTHYKMPRE
                     IHFVQSIPRTASGKILRRDLRKLF"
     gene            4504..5664
                     /locus_tag="VFC15_05060"
     CDS             4504..5664
                     /locus_tag="VFC15_05060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM09561.1"
                     /translation="MYYGALISGVVCLAIWIYLLVAHGMFWRVSSLRANVQLANELDG
                     TIAVVIPARDEADVVGATIQSLLRQTRLDSVHIFVVDDHSSDGTAEVAREAARSCGQS
                     ASLDVITGSTLPAGWTGKLWAVEQGIEQAMRLRPGFLLLTDADIQHSPDNLATLVAIA
                     EAGNYDLTSFMVKLHCKTVAERFLVPPFVFFFFLLYPPAWIRDAQRKIAGAAGGCMLI
                     RPHALEQAGGMAAIRNQIIDDCALARAVKQSGGRVWLGVTPDACSTRVYRTFGEIERM
                     IARTAFNQLQHSAWLLFGAMVGLAITYLLPLALLLSGNVTLAVVGVAGWVLMSAAFLP
                     MVRFYGLNPAWALTLPLSACFYMLATIHSAFKFWSGRGGEWKGRAQDVASTD"
     gene            complement(5661..6143)
                     /locus_tag="VFC15_05065"
     CDS             complement(5661..6143)
                     /locus_tag="VFC15_05065"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZM09562.1"
                     /translation="MVIILMGVTGSGKTTIGQALAAALQWRFVDADAFHSTANVAKMR
                     AAIPLDDADRLPWLTSLHDAIDGWLQAGTNVVLACSALKQTYREQLLVSRDVKLVYLR
                     GSRELISQRLSQRHGHYMDPNLLPTQFATLEEPREAVVADVDAAVPDSVAHIRKALGI
                     "
     gene            complement(6147..6992)
                     /locus_tag="VFC15_05070"
     CDS             complement(6147..6992)
                     /locus_tag="VFC15_05070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM09563.1"
                     /translation="MADVNPAKRVALVLCDIDGTLLDNDKKLTPGAPAAVLRLYDAGI
                     RFTLASARPPRMTRELVRVLQVREPLACFNGALIEGPDETVLRQTRIAPSNAQAVADQ
                     IVRSGFDLWVWTDTDWYVSNPNGPHVEHHESQMGSKATPLPSHDLSQFHVLKLVGVSD
                     DYEALAEAEKKMSALPHSSISATRSSPYYLDVTDAKANKGQVALTLSEMLHIPTSQIA
                     TIGDMNTDALMFRQTGVSIAMGNALNDVKAQAKFVTKSNEEDGFAYAMDHFVLGFAEK
                     QAAVD"
     gene            complement(6992..8548)
                     /gene="zwf"
                     /locus_tag="VFC15_05075"
     CDS             complement(6992..8548)
                     /gene="zwf"
                     /locus_tag="VFC15_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZM09564.1"
                     /translation="MSTATQPETFKITQPSLPKAGSCLLVIFGGTGDLTRRKLIPALY
                     DLACIGCTNPNFVVLGVGRTELTDEQFRERMHEGAAKSKDARNYSEDGWSDFASRLHY
                     LVGDANHPDFYPVLKTKIAEMQKDGASENVLFYVSTPASLAPPIVEGLGQAGLAHHGK
                     GWTRIVLEKPFGHDLKSAQDLNAIVSRVFDEESVYRIDHYLGKETVQNIMVFRFGNSM
                     FEPIWNRNYIDYIEITAAETLGVESRAAFYEETGALRDMVANHLLQLLTLTAMEPPVS
                     FDANSVRELKVQVLRAIRPMSVDEVAKHTIRGQYGPGKINGVEVPGYRQEPGVNPNSH
                     TETYVAVEFHVDNWRWAGVPFYVRAGKRLANQLTEIAVHFKRTPQALFARNPGDVHHN
                     VITMRIQPNEGISLNFGAKTPGTQMRASRVNMDFSYRDTFGTATPVAYETLLLDAMRG
                     DATLFTRRDEVEAEWRIITPIEEAWSQLPEPQFPNYAAGSEGPENANSLIQYDRQRWR
                     SLSRDDSGDL"
     gene            complement(8545..10017)
                     /gene="gndA"
                     /locus_tag="VFC15_05080"
     CDS             complement(8545..10017)
                     /gene="gndA"
                     /locus_tag="VFC15_05080"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HZM09565.1"
                     /translation="MPATTAVRTAQFGVIGLGVMGANLALNVEDHGHTVAVWNLETEW
                     VDRLLQKNPGRQIIGAKTLQEFCRALERPRRILIMITAGDPVDQTLQRLSPYLAPGDV
                     VIDGGNSYFKDTIRREANARQIMVKYFGMGVSGGEEGARHGPSLMPGGPRDAYNHIAP
                     IVQSIAAKSDSGPCVSYVGPDGSGHFVKMVHNGIEYGDMQLIAEAYEILNKALHLSAQ
                     QLSQIFAEWNRGPLESYLIEITAKIFGVTDPETGKPLVDMILDKAGQKGTGKWTAQIA
                     LELEVPVPTITAAIDARALSGMKEERVIASQLIHSSAGSVFSGEKRDLIQAMHDALYA
                     SKICSYAQGMSLIRAGSDEWQWDINLREMARIWKGGCIIRARFLDSIMRAFERQPDLP
                     NLLLDTEFMTWMRRAEPSWRKLIATAVQMGIPVPALSASLSYFDSYRSAQLPQNLTQA
                     QRDFFGAHTYQRADRPTAGFVHTDWTTLFAKPSRPPGGTK"
     gene            complement(10074..12143)
                     /gene="tkt"
                     /locus_tag="VFC15_05085"
     CDS             complement(10074..12143)
                     /gene="tkt"
                     /locus_tag="VFC15_05085"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZM09566.1"
                     /translation="MSSQASVPTQDNKQVIAAAKAEQQLEQLCINTVRTLSMDAVQKA
                     NSGHPGTPMALAPLAFVIWDRFLRFNPRNPHWPGRDRFVLSNGHASMLLYSMLYLTGY
                     DLTLDDLKSFRQWGSKTPGHPEYGLTAGVETTTGPLGQGISNSVGMAIAQRWFASQFN
                     RDGHDLFDYRIYSICGDGDLMEGVSNEAASIAGHLGLSNLIWFYDNNHITIEGNTSLA
                     FSDDVATRFLAWHWNVQHVGDVNDLAALDRAIREAQKETQRPSMIIVNSHIGWGAPDK
                     QDTAEAHGEPLGVEEIRLTKVNYGWPPDAQFLVPEEAKSYMGKAVDRGAQWEQEWDNK
                     YKTWAQAFPELAKEWNLIAKRELPQGWDKDIPTFPADAKGIATRESNNKVLNAVAKNV
                     PWLIGGAADLAPSTKTLIKDAGSFERDEYSGRNFHFGIREHAMGSIVNGMCVSGVRAY
                     GATFFIFSDYMRPAIRLAALMDLPAIFIYTHDSIGLGEDGPTHQPIEQLMSLRAMPRL
                     MVFRPADANEVSETWRYVMQLQHEPVALALSRQAMPTFDRTKYGSAAGVARGAYVLAD
                     CKGAPDVILMGTGSEVQLCIGAYEKLTADGVKTRVVSMPCWELFAKQPAEYKAEVLPA
                     VVKARVAVEAGATFGWKEYVGDDGEVVGRDDFGASAPIKDLMTHFGFTVDNVVAKARE
                     AISKTKS"
     gene            complement(12177..14567)
                     /locus_tag="VFC15_05090"
     CDS             complement(12177..14567)
                     /locus_tag="VFC15_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZM09567.1"
                     /translation="MGNSNPAPGGPGIPPRWTRGAKDAVGTAYAVSSRVWYTIANGVI
                     TEVYYPTIDTPQIRDIQYLVTDGETFFHDERRNMQSQLDCVGAAALGFKITNSDPEGR
                     YSIEKQIIGDPHLSCLLVHTRFNVAPAWQGKLHLYVLCAPHLEIGGLHNNGQVAETRG
                     RHFLMAYRSFSYLAIAATLPFSRLSCGYVGVNDGWTDLSHNFQMDHEYDQALDGNIAL
                     TAALDLSQGTEFTLGVGFGHTRHSAATTVYQSLSISFDAKLQKFNDQWNRTSKRFVLL
                     DKVAEHDSTLFERSVNLLLAHEDKVYPGAMIASLSIPWGENKGDADLGGYHLVWTRDM
                     VSCASALLAVGDTTTPLRALVYLAMSQREDGGFYQNFWIDGRPYWTGTQLDEVSFPIV
                     LAWRMWRADALRDFDPYVTVQRACGFLIREGPATAQDRWEEAGGYSPSTLASNIAGLI
                     CGAEFLEARGDKHTAAFVRTYADFLESHVEKWTVTNHGTLVPGIERHYIRINPAVSSH
                     GSCGDEDPDQGVVTLANQRPGEQSKYPAKDIVDAGFLELVRFGIRMPDDLLVEQSLKV
                     VDAILKVDTPNGPCWKRYNHDGYGQRDDGGPFDGWGVGRPWPLLTLERATYELSAGHN
                     IDPYLKAMSNFSTGIGLIPEQIWDAPDLPEAYMYFGKATGAADPLMWAHADYLRLLRS
                     IADGRIFDLVDPVAERYRNDKPRPVIEVWKMNRQVKCVPAGALMRIQASSAFLLHWSD
                     DEWTTKHDSASIPTSIGVEYADVQVASSQTAPIRFTFYWTQQAQWQGEDYSVDIAR"
     gene            14952..>15570
                     /locus_tag="VFC15_05095"
     CDS             14952..>15570
                     /locus_tag="VFC15_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZM09568.1"
                     /translation="MSSIINTPKGLEGVTAVNSSVCYIDGDQGVLAYRGIDIHELADH
                     SNFEECCYLLWFGHLPTRAQLLELKLNMARERKLDASIISLLNQAPKHALPMDVLRTT
                     VSALSYYDPEEKVNDAEANVRKTIRLTSQIAYVVAAYDRIRKGRHVIEPDRALSHAAN
                     FLYQLNGEIPSATAERALDIALILHADHELNASTFAARVVAATLSD"
BASE COUNT         3038 a         4613 c         4574 g         3345 t
ORIGIN      
        1 gcgtaagtcc acgcccacca cgcgaaactc cgacaacatc ggcaggagcc gagtcccgag
       61 gttccccgca attcccgtca ccaccaccgt cggctgtgtc atcgaattac tggggcggat
      121 tgccggattg gggtggacca ccctggcccg gccgccctcc cgttgcagga gcagccgcag
      181 gcgggggagg tccgaaatac tcgtcgttca acaccggcgt ggcggatttc ccaattgcat
      241 ccaacttttc ctgcaattgc tgctgctgca gtgtcttcac gatcgagtcc ttaacctcgc
      301 tcaatggaat ttgccgtacg gtcacgacct tgtaaatgta agccgctgcc gggtcgctat
      361 aggtttgcga aatctcacct gccttcagtt ggaatacgct ttcctgattc actggcagac
      421 tgcccgggcg cctcgctccc agattgattt caggcgtaga tgctccggtc atgccggctg
      481 cctcgaaagc tgactgctgg agcttgaccg ggtcttcacc tgcaacccag cgctgccgaa
      541 tcttttcagc cgcgacggcc gcgtctgctt cgctcagggc cggcttgtcg gccgatccgg
      601 ggtttttcgg gacgatgatc cgttgcaggg tgacttcgag gtacttggag ctgttctggt
      661 tgtaatagtc ctggatctgc tggtcgctgg gatgcgcgaa ctgttccgtg taatggcgct
      721 ttagcccctc agccaggact tgttgactca caaactgaat aatctgctgt acagttggat
      781 cgttctccag atgcatggct cgagccgcat cgaaaaagac cagaaggcgt ccataattcg
      841 tcgcaaatcc ccgcttggct tcagcggcca tgtcaggttg caatgcattg atgatcttct
      901 cgaattgtgc gcgggtgatg ctgctgacac aatccttggc aggggcaatg tcgcctatcg
      961 gctcgcagcc gccctttaat gtgatcaccg gctggtccat tccaactgtg ctagggttag
     1021 ctgccgacgc gccgctgttg gcgcgactgg ccacggcggc tcgtttctcg gcgcgcgtgg
     1081 ggggtggcag cggagtgccc ggactatcgg tttgtgcgta accagcagtg atgagaacac
     1141 acgaaagcgc aagaaccgtt atgatctgac gttgcatgaa gggtcttcct ccgggaaagg
     1201 gtcgcttgtg gcgccgtggc agttacacaa aatcgtatca taggatgccg ggctcatcgc
     1261 cgtcgaagtg ggccggtgtg aactgtcgac acttgtatgc ctgagtccgt caatatcgat
     1321 gcgcgtcagt cggagaaaca gtcagtcgcc ctgaactctg tctttgccgc agtggcgatc
     1381 accagtttga agatcgtcgt gggaatctcc acgggtagcc tgggaatact gtccgaggct
     1441 gcacactccg gcctcgacct tgtggctgcg gcgatcacct acctgtctgt tcgcgtctct
     1501 gacaagcctg ctgacgccga tcaccaatat ggccatggaa aagtcgagaa cttctccgca
     1561 ttcatcgaaa ccggcctgtt gttgctgact tgcctctgga tcgtctacga agccaccaaa
     1621 cgattgttct ttcactcggt tgagattgaa cccagcgttt gggccttcgt cgtcatggct
     1681 atttcaatca tggttgattt ctggcgctcc aagcggctga agcgaattgc cgacaagtat
     1741 gacagccagg ccttgctggc ggacgctctc catttcggca ccgacatctg gtccagtgcg
     1801 gtggtaattc tcggcttgag tctggtgatg gcggggcgca catattccgt cccttggttg
     1861 gcaaaagccg atcccatcgc ggccctggtg gttgcttgca ttgtggtgta tgtcagttgg
     1921 cgcctggcgc ggcagaccat tgacgctctt ctcgacggcg ctccggcagg agtgcgctcc
     1981 aaaatcatcg acgcagtttc ccgcgttgac ggagtgctgg agatcgatcg cgtgcgcatt
     2041 cgacgcggcg gcagcaaata tttcgccgat gtctccatcg ccatgtcgag gagcgtgacc
     2101 ttccagaaat cggagcaggt tgccaacgag gtttcatcgc gaatacggga gcttttgccg
     2161 gaagccgata tcgtggtcaa cgctgttgcc cgcgccagtc gcggtgaaag cctgttcgat
     2221 cgcattcgcg ccgtggccac caggaacaac ctcaacgtgc acgacatcag tgtgcaagac
     2281 atccatggcg cgctgcatgt ggagcagcat gttgaactgg atgagcgcat gccgctcaag
     2341 gacgctcacg accgggtcac acggctggaa agcgagatgc ggcgcgacat tcccgaaata
     2401 tcgagcatcc tgactcacat cgagagtgag cccgctacca tcgaaaccag cgacggcgtg
     2461 gtggatgccc caagcttcga acgccgtatt cagttggcca cgggggcttt ccctgaggtc
     2521 gtcgacgcgc atgacctgat gtttaagcgg gtggcgggac ggctctacct atcgctgcac
     2581 tgcaccatga aggatgacct tccgctggca cgggtgcatg acattcagac agcactggag
     2641 agccggttcc ggcaggaagt gcccgacctg tttcgcgtgc tgatccatcc tgagccgcag
     2701 accgacaacc gacgttaggc tcggacctta aaacaatttc cgcaaatccc gccgcagaat
     2761 cttgcccgag gccgtccggg gaatgctctg aacgaagtgt atttcgcgcg gcattttgta
     2821 atgcgttagc cgctcggcta caaagtcacg aatgctatcc gttgtttgtt ccgtttcaag
     2881 gtggctttca cgcaacacta cgaacgcgca gggaatttcc tctgatgcct catcgacccg
     2941 tgaaaccaca ccgcagtcgc gcaccgcggg atgctcgagg aggacgcctt cgacctctgc
     3001 tggggcgatc gagaagcctt tgtatttcat catctccttg cgccggtcga cgacgaagta
     3061 cagaccttgc tcgtccaccc ggacgacgtc gccggaccag taccacccgt catgcatcac
     3121 ggcggcagtg gcatccggtg acttccaata tcccaacatg aattgggggc cacgcatgac
     3181 cagctcgccg agttctcctt gcgggacatc gttgccgttt tcgtccagca cgcggcattc
     3241 cgtctgggca acaggaccgc caatcgaggc gctgttatag aactgcggat cgaggtagcc
     3301 catatgcgtc accggcgaag cttcggtcat gccgtaccct tgtcggatga gtatgcccgt
     3361 cgcctccgtg aagcggcgcg cgaggtccgg tgccagcggg gcagcgccgc atttcaccca
     3421 gcgcagacca tgctcgcgcg gaaacttccc cgcatcacac gcgtgacaat aagccaacaa
     3481 cgcgggcgga acgcagaggg ccatggtcac accttcatcg acaatggccc gcagcgaacc
     3541 ttcgcagtcg aatcgcggca tcaggacaac cgtgcacccg cgcatcaggc agaggttcat
     3601 gccgaccgtc attccgtaga tgtggtacat cggcagaaaa cacagcacca cgtcgctttc
     3661 gccgatgcat cctgtttccc ccgatgtcag cgtctggtac acgttggcca ccagattgtg
     3721 gtgcgaaagc atcacgccct tcggcaatcc cgtggtgccg ctggagaagg gcagagtcgc
     3781 aatcgtcaat cgtgagtcat gctcgggcgt gggcaaactg acgcgcgcag ttgaagagta
     3841 gagtttgctc atcggctcgg aaccgcccgg tgttgcggtg cggatcgtga atacattccg
     3901 caacccgggc agacctgaca ggtcgatgcc ctccagcaat ggtccgtcac taatcagcgc
     3961 caccgcatcc gaagtctcca tctggtgatg cacttcacgc tcgcgataag ttgggttcat
     4021 cgtggttgga atggctccca ccatggtcgc ggcatgaaac gcgaccccga attcccagca
     4081 attcggcaag aagatgccaa tcatctcacc cggacggatg ccggcgacga cgaacccgtg
     4141 agctgtgcgc tcaaccagtt cggcgtactc gccatagctg atgcgccggg gcggcgtaca
     4201 agacatgtcc acgatggcga tcttgtcgcc atgctgcctg cacgactcga gtaccacgtc
     4261 gtggacgaac ttaccgtcgg gattggcgta gagttgggtg cgaagtggag ccatgggtag
     4321 tgtcataggg aaccgccgga gagtgtagca ggttgagggc ggactgcaac agatggattt
     4381 tcgcgcggtg atccggatcg cgggagggaa gagctgtctg acgcggtccg cgtcttcgca
     4441 tgatcaaccc ggcaattttc ctggatccga tcggccactc ggttccggat aaactaaaac
     4501 cgaatgtatt acggcgcgct catcagcgga gtagtttgtc ttgctatctg gatctacctg
     4561 ctcgtagccc acggcatgtt ctggagagtg tcgagccttc gcgctaatgt gcaactggca
     4621 aatgaacttg atggcacaat tgcggtggtc atcccggctc gcgacgaggc ggacgtcgtt
     4681 ggtgcgacga tacagtcact gctgcgacag acgcgtctcg attccgttca tatcttcgtg
     4741 gtcgatgatc acagctccga cggaactgcc gaggtcgcgc gggaagcagc tcgcagttgc
     4801 ggacaatccg cgtctctgga cgtgattacg ggcagcacac tgcctgcggg atggacgggt
     4861 aaactctggg cggtagagca ggggattgag caggccatgc ggttgcgccc gggtttcctg
     4921 ctgttgaccg acgctgacat acagcactcg cccgacaatc tcgcgacgtt ggtagcgatc
     4981 gccgaagcag gaaactacga cctgacgtcg tttatggtga agttgcactg caagaccgtc
     5041 gcagagcggt tcctggtccc gcccttcgtg ttcttcttct tcctgctata tccacccgcc
     5101 tggattcgcg atgctcaacg caaaatcgct ggagctgccg gaggctgcat gctgattcgc
     5161 ccacacgcct tggagcaggc gggcggcatg gcagcgattc gcaaccagat catcgatgac
     5221 tgcgcgttgg cgcgggctgt caagcaatcc ggcgggagag tgtggctcgg tgtgacccca
     5281 gatgcctgca gcactcgcgt ttacaggacc ttcggcgaaa tcgaacggat gatcgcccgc
     5341 accgctttca accaattgca gcattcggct tggttactgt tcggcgcgat ggtcggcttg
     5401 gcgataacgt atctactccc cttggcgctg ctcttgagtg gcaacgtgac tctcgccgtc
     5461 gtgggagtgg caggttgggt gctgatgtcc gccgcttttc ttccgatggt gcgattttat
     5521 ggcttgaatc cggcatgggc gctcaccctg ccgctcagcg cctgcttcta tatgcttgcg
     5581 acaattcatt cggcgttcaa gttctggtcg ggccgcggcg gggagtggaa gggccgtgcc
     5641 caggacgtcg cgtcaacgga ctagatgccg agcgccttcc gtatgtgggc aacgctatcg
     5701 ggcacagctg catctacatc cgccaccact gcttcgcgag gttcttcgag cgtggcgaat
     5761 tgagtgggca gaagattcgg gtccatgtag tgcccatggc gttgcgagag tcgctgagaa
     5821 atcagctcgc gactgccgcg caggtaaacc agcttgacat cgcgactgac cagcaactgt
     5881 tcgcggtagg tttgcttcag cgcggaacac gccagcacga cgttggtccc cgcttgcaac
     5941 cagccgtcaa ttgcgtcgtg gagagaagtc agccacggga gccggtcggc atcatccagc
     6001 ggaatggccg cccgcatctt ggccacgttc gcagtcgaat gaaaggcatc ggcgtcaaca
     6061 aaccgccact gcagagccgc ggccagagcc tgaccaatcg tggtcttgcc tgatcccgtc
     6121 actcccatga gaatgattac catcgctcag tcgacagcgg cttgcttttc cgcgaacccg
     6181 agcacgaaat ggtccatggc gtaggcaaaa ccgtcttcct cattgctctt ggtcacgaac
     6241 ttggcctgcg ccttgacatc gttaagggcg ttgcccatgg cgatgctgac gccggtttgc
     6301 cggaacatga gagcgtcggt gttcatgtcg ccaatggttg cgatctggct ggtgggtata
     6361 tgcagcattt cggagagcgt cagggcaacc tgccctttgt tggccttcgc atcggttacg
     6421 tcgagatagt acgggctgga gcgggtggcc gagatggacg aatgggggag ggctgacatc
     6481 ttcttttcgg cctcagccaa cgcctcataa tcgtcactca ctccaaccag cttcagtaca
     6541 tgaaactggg acaaatcgtg ggatggcaga ggagtcgcct tactgcccat ctggctttca
     6601 tggtgctcga cgtgcgggcc gtttggattg ctgacgtacc aatccgtgtc cgtccacacc
     6661 cacaggtcaa acccgctccg aacgatctgg tcagcaactg cttgggcgtt cgacggcgca
     6721 atgcgcgttt ggcggagcac ggtttcgtca ggaccctcaa tgagagcgcc attgaagcag
     6781 gcaagaggct cgcggacttg gagcacgcga accagctcgc gagtcattcg tggaggcctc
     6841 gcgctggcca gtgtgaatcg tattccagcg tcatacaggc gcagtaccgc ggctggagcg
     6901 ccgggcgtga gcttcttgtc gttatcgagt agcgtaccgt cgatatcaca cagcacgaga
     6961 gccacgcgtt ttgccggatt cacgtcagcc atcagaggtc cccgctgtca tccctgctta
     7021 aggaacgcca gcgctggcga tcatactgaa tgagagagtt ggcgttctcg ggtccttcgc
     7081 ttcccgcggc ataattgggg aactgcggtt cgggaagctg cgaccacgcc tcctcgatgg
     7141 gagtgatgat tcgccactcc gcttcgacct cgtcacggcg ggtaaacaac gtcgcatcac
     7201 cccgcatcgc atccagcaac aacgtttcat aggccacggg agtggccgtg ccaaacgtat
     7261 cgcgatagga aaaatccatg ttgacgcggc tggcacgcat ctgcgtcccc ggtgtcttgg
     7321 ccccgaagtt cagagagatg ccttcgttcg gctggattcg catggtgatg acgttgtgat
     7381 gaacgtcgcc cggatttcgc gcaaacagag cttgtggcgt gcgcttaaag tggacggcaa
     7441 tttcagtgag ctggtttgcc aatcgtttgc cggcacgcac ataaaaggga actcccgccc
     7501 agcgccagtt atccacatga aactcgaccg ccacgtaggt ttctgtgtgc gaattgggat
     7561 tcacacccgg ctcctggcga tagcctggaa cttccacgcc gttgatcttg cccggcccgt
     7621 actgtccacg aatggtgtgc ttcgccacct cgtccaccga catcgggcga atggcgcgca
     7681 gcacctggac cttcaactca cgcaccgagt tggcgtcgaa cgacaccggc ggctccatgg
     7741 ccgtgagcgt cagcaattgc agtaggtggt tggccaccat gtcgcgcagc gcgcccgtct
     7801 cttcatagaa cgcggcccgg ctctcgacac ccagtgtttc agcggctgtg atctcgatgt
     7861 agtcgatgta attgcggttc cagatgggct cgaacatgga gttgccaaag cggaacacca
     7921 tgatgttctg gaccgtttcc ttgcccaggt agtggtcaat gcgatagacg gattcctcgt
     7981 cgaacacgcg tgagacgatc gcattcaggt cctgggcgct cttcaagtcg tgcccaaagg
     8041 gcttctccag tacgatgcgg gtccatcctt tgccgtgatg cgcgagtccg gcctggccaa
     8101 gcccttccac gatcggcgga gccagggatg ccggcgtcga aacgtaaaac agcacgttct
     8161 cgctggcgcc gtccttctgc atctctgcga ttttcgtttt caagaccgga tagaagtcgg
     8221 gatggttggc atctccgacc aggtaatgca ggcgactggc aaaatcgctc cagccgtcct
     8281 cgctatagtt gcgagcgtcc ttcgacttcg ctgcgccttc gtgcattcgt tcgcggaact
     8341 gctcatccgt gagttccgtg cgtccgacgc cgaggacgac aaagttcggg ttggtacacc
     8401 cgatgcacgc caggtcatac agtgcgggga tgagcttgcg gcgggtcagg tctccggtgc
     8461 cgccgaaaat aaccagcagg cacgaacctg ccttgggcaa gctgggctga gtaattttga
     8521 atgtctcggg ctgtgttgcc gtactcactt cgtacctccg ggcggtcggg aaggttttgc
     8581 gaacagggtt gtccagtccg tgtggacgaa gccggcggtc ggacgatcgg cccgctgata
     8641 ggtatgggcc ccaaaaaaat cgcgctgcgc ctgggtcagg ttctgcggca gctgcgcgct
     8701 gcggtagctg tcaaaatacg agagtgaagc ggaaagcgcg ggaaccggaa tccccatttg
     8761 caccgcggtt gctattagct tgcgccaaga cggctccgcc cggcgcatcc atgtcatgaa
     8821 ctcggtgtcc agcagcagat tgggaaggtc gggctggcgc tcgaacgcgc gcatgatcga
     8881 gtccagaaag cgcgcccgga taatgcagcc acccttccag attcgcgcca tctcgcgcaa
     8941 attaatgtcc cactgccact catccgatcc ggcacgaatc aggctcatgc cttgcgcata
     9001 ggaacagatc tttgaggcat acaaggcgtc gtgcatcgcc tggatcagat ctcgtttttc
     9061 tccgctgaat acgctgccag cagaagagtg gattaattgg ctcgcaatca cccgttcttc
     9121 cttcatgccg gagagcgcgc gtgcgtcgat cgccgcggtg atggtaggca ccggcacctc
     9181 aagctcgagc gcgatctggg ctgtccactt gcccgtgcct ttctgccccg ctttgtcgag
     9241 gatcatgtcg accagcggct taccggtctc aggatcggtc acgccgaaga tcttggcggt
     9301 gatctcgatt agatacgact ccagcgggcc tcggttccat tccgcgaaaa tttgggagag
     9361 ttgctgcgcg ctcaggtgta gggccttgtt caggatttcg taggcctcgg caataagttg
     9421 catgtcgccg tactcaatgc cgttgtgcac catcttgacg aagtggcccg agccgtccgg
     9481 tccaacgtag ctaacgcacg gtccggaatc gctcttcgcg gcgatggact ggacgatggg
     9541 agcaatgtgg ttgtaggcgt cgcgcgggcc gccgggcatc agggatggtc cgtggcgagc
     9601 gccctcttcg ccgccggaga cgcccatgcc gaaatatttg accatgatct ggcgggcgtt
     9661 ggcttcgcgg cggatggtat ccttgaaata ggaattgccg ccatcaatca cgacgtcgcc
     9721 cggtgcgagg tagggagaaa gcctttgcag cgtctggtcg acgggatcgc ccgccgtgat
     9781 cattatcagg atgcggcgag gacgctccag cgcgcggcag aattcctgca gcgtctttgc
     9841 cccaatgatc tgccgtcccg gattcttttg gagcagacgg tcgacccact cggtctccag
     9901 gttccacacc gccaccgtgt gcccatggtc ctcgacattc agggcgagat ttgcccccat
     9961 cacgccgagg ccaataacgc caaactgcgc tgtcctgacc gcggttgtcg ccggcatgtg
    10021 tgtcgcctcc ttgcgtgcgt tgggtctcgc ggcggcaagc gcgcctcgta aaactacgac
    10081 ttcgttttgc tgatggcctc acgcgctttg gctaccacgt tgtcaaccgt aaatccaaag
    10141 tgcgtcatca aatccttgat gggagccgat gccccgaaat catcgcgccc cacaacttca
    10201 ccgtcgtctc caacatattc cttccatccg aaagtagctc cggcttccac cgccacccgg
    10261 gctttcacca ctgcgggcag gacttcagcc ttgtactctg ccggctgctt tgcaaacagc
    10321 tcccagcaag gcatcgaaac gacccgcgtc tttacgccat cggcggtgag cttctcgtag
    10381 gccccgatgc agagctggac ttcggagccc gtgcccatca gtatgacgtc cggggcgccc
    10441 ttgcaatcgg ctaacacata cgcgcccctg gccacacccg cagcggagcc atatttcgtg
    10501 cggtcgaatg tcggcatagc ctggcggctg agcgcaagcg ccacgggctc gtgctggagt
    10561 tgcatgacgt atcgccaggt ctccgagacc tcgttggcgt ctgcgggacg aaagaccatc
    10621 agccggggca tcgcccgcag cgacatgagc tgttcgatgg gttggtgggt cggtccgtct
    10681 tcacccagcc cgatggaatc atgggtatag atgaagattg ctggcaggtc catcagtgcg
    10741 gccagacgga tggccggcct catgtagtcg gagaaaatga agaacgttgc cccgtacgcg
    10801 cgcacccctg acacgcacat accgttcacg atcgagccca tggcgtgctc gcggatcccg
    10861 aagtggaaat tgcgtcccga gtattcgtcg cgctcgaaac ttccagcatc cttgatcaat
    10921 gtcttggtcg aaggcgcgag atcggcagcg ccgccgatca gccatggaac attcttggcg
    10981 accgcattta gcaccttgtt attcgattcg cgggtggcga ttcctttggc atcggcgggg
    11041 aacgtgggaa tgtccttgtc ccagccttgc ggcagttcgc gcttcgcgat caggttccat
    11101 tcttttgcga gttccggaaa cgcctgcgcc catgtcttgt atttattgtc ccactcctgt
    11161 tcccactggg cgccgcgatc gacggccttg cccatgtagg atttggcttc ttccggcact
    11221 agaaattgcg cgtccggcgg ccaaccgtag ttgactttgg tgaggcgaat ctcctctaca
    11281 cccagaggtt cgccgtgggc ttcagccgtg tcctgtttgt caggagcgcc ccacccaatg
    11341 tggctgttga cgataatcat ggatggccgc tgcgtctctt tctgcgcctc ccggatggcg
    11401 cgatccaatg cagccaaatc gttgacgtcg cccacgtgct ggacgttcca gtgccacgcc
    11461 agaaaacgcg tcgccacatc atcggagaaa gccagtgaag tattgccttc aatcgtgatg
    11521 tggttgttgt cgtagaacca gatgagattc gacagcccaa gatgtccggc aatcgacgcc
    11581 gcctcattgg agacaccttc catcagatcg ccgtcgccgc aaatcgagta gatgcggtag
    11641 tcaaagagat cgtgtccgtc gcggttgaac tgcgacgcaa accagcgctg cgcaatcgcc
    11701 atccccaccg agttgctgat gccctggccc agcggcccag tcgtagtctc gacacctgca
    11761 gttaagccat actccgggtg cccaggcgtc ttcgatcccc actgccggaa gctcttcagg
    11821 tcgtccagcg tcaaatcgta gccagtgaga tacagcatgg agtagagcag catcgaggca
    11881 tggccgttgg agagcacgaa gcggtcgcgg cccggccaat gaggattgcg aggattaaag
    11941 cgcaggaagc ggtcccagat cacgaaggcc agcggcgcca gcgccatcgg ggttccggga
    12001 tggccgctat tggctttctg cacggcatcc atggaaagcg tacgaaccgt gttgatgcag
    12061 agctgttcga gttgttgctc cgccttggcc gcggcgataa cttgtttatt gtcctgcgtg
    12121 ggaacactgg cttggctact cactggtggt cacctttctt tctggtatcg gttcgttcac
    12181 ctggcaatgt cgacgctgta gtcctcaccc tgccactggg cctgctgggt ccaataaaaa
    12241 gtgaagcgta taggagcggt ttgggatgat gcaacttgga cgtccgcgta ttcgacgccg
    12301 atcgaggtcg ggatagatgc gctgtcgtgc ttggtcgtcc attcgtcatc gctccaatgc
    12361 agcaggaacg ccgaagatgc ttgtatcctc atcaaggctc cggcaggtac gcacttgacc
    12421 tggcggttca tcttccatac ttcgatgacc ggccgcggct tgtcgttgcg gtaacgctca
    12481 gcgaccgggt cgaccaggtc gaaaattctt ccatcagcga ttgaccgcag tagtctcaaa
    12541 taatcggcgt gcgcccacat caggggatcg gctgcacctg ttgccttgcc aaaatacatg
    12601 taggcttccg gaaggtcagg cgcatcccag atttgctcgg gaatcaaccc tatcccggtg
    12661 gaaaaattac tcatcgcctt cagatatgga tcgatgttat gccctgcgga cagttcgtag
    12721 gtggcgcgtt ccaaggtcag cagaggccat ggtcttccca cgccccaccc gtcgaatggg
    12781 ccgccgtcat cgcgctgacc gtatccgtcg tggttgtagc gcttccagca gggtccattg
    12841 ggcgtatcaa ctttcaaaat agcgtcgacg actttcagcg attgttccac cagaagatcg
    12901 tcaggcatac ggatcccaaa gcgcaccaac tccagaaatc ccgcgtccac gatgtctttc
    12961 gcaggatact tcgactgctc gcccgggcgc tggttcgcaa gagtcacgac accttggtca
    13021 ggatcttcat cgccgcaaga gccgtgcgaa ctcactgcgg ggttgatgcg aatatagtgc
    13081 cgttcgattc ccggaaccag tgttccgtga ttggtgaccg tccacttttc gacgtgggac
    13141 tcgaggaagt cggcataagt gcggacgaac gcggcagtgt gcttgtcgcc ccgagcttcg
    13201 agaaactccg cgccgcaaat cagtccggca atattggacg ccagcgttga aggcgagtag
    13261 ccgccggctt cttcccatcg gtcctgggcc gttgccggtc cttcacgaat caagaatccg
    13321 caggcccgtt ggaccgtcac gtaggggtcg aagtcacgga gggcgtccgc tctccacatc
    13381 cgccatgcca gcacgatggg gaacgatacc tcgtccaact gcgtgcctgt ccagtagggc
    13441 cgtccgtcga tccagaagtt ctgatagaaa ccgccatcct cgcgctgcga catggccagg
    13501 tacaccagcg cccgcaaagg tgtggtcgta tcgccgaccg caagtagggc agacgcgcag
    13561 ctcaccatgt cccgcgtcca caccaggtga tagccgccga ggtcggcgtc gcccttgttt
    13621 tcgccccagg gaatgctgag gcttgcgatc atcgcgcctg gatacacctt gtcttcgtga
    13681 gcgagtaata agttcacgct acgctcgaac aaagtcgagt catgctcggc aactttatcc
    13741 agcagcacga agcgcttgct ggtgcggttc cactgatcat tgaacttttg cagcttggca
    13801 tcaaaggaaa tgctcaacga ctgatagacc gtggtggcgg cactgtggcg ggtgtgtcca
    13861 aagcccacgc cgagggtgaa ttcggtccct tgcgagaggt cgagggccgc ggtgagcgca
    13921 atgttgccgt ccagtgcctg gtcgtactca tggtccatct ggaagttgtg ggaaagatcg
    13981 gtccaaccgt cattcactcc gacataacca caagaaagac gcgagaaagg aagggttgcc
    14041 gcaatcgcta agtaagaaaa actgcggtag gccatgagaa aatgccgccc ccgcgtttct
    14101 gccacttggc cgttattatg cagccctccg atctcgagat gcggagcaca cagcacgtag
    14161 agatgcagtt tgccctgcca cgccggagct acgttgaagc gcgtatgcac gagcaggcaa
    14221 ctcaggtgcg gatcgccgat gatttgcttt tcaatgctgt agcgtccctc aggatcggag
    14281 ttcgtgattt tgaagcccag cgccgcggcg cccacgcagt ctaattgcga ctgcatgttc
    14341 cggcgctcgt catggaagaa agtctccccg tccgttacca ggtactgaat gtcgcggatt
    14401 tggggggtat cgatggtggg atagtaaacc tcggtgatca cgccattggc aattgtgtac
    14461 cagacacggc tggagaccgc gtaagccgtg cccaccgcgt ccttggctcc gcgcgtccat
    14521 cgcggcggaa tcccgggccc gcctggcgct gggttgctat tccccatcac acgctctctc
    14581 ctgatggttt ggtacctgag gtcggccgat agccgcacgg gatcgtagcg tggggttatc
    14641 tcagacagcc cctcacatgc acggcagcca gcgccatagt gtaacccgag accacgacca
    14701 gggagtcgtg cctggccaca atctgtgtca ggagcgacgg acagcgcgcc ccggtcgcga
    14761 taagcgcacc tctaaattcg tgcctttgga aggtcggcag caagaaactg tgacgggtga
    14821 actggactgt ttcgcgcgtg gggagtacaa aagttggtta cggaaatttg tgactctgcg
    14881 gagcacctgg ccgcgcagcc tgcctttaaa tcttaaatac gtgcggttcc acggaactga
    14941 gcgaggattt catgtccagc atcattaaca ccccgaaggg cctggaaggc gtgaccgccg
    15001 tcaattccag cgtctgctac atcgatggcg accagggcgt actagcctat cgcggcatcg
    15061 acattcacga gcttgccgac cactcgaatt tcgaagaatg ttgctacctg ctttggtttg
    15121 gccatctgcc cacccgcgcc cagttgctgg agttgaaact gaacatggcg cgcgagcgca
    15181 aactcgatgc gtccatcatc agcctgttga accaggcgcc taagcatgcc ctgcccatgg
    15241 acgtgttgcg cactaccgtt tccgcgctct cgtactacga tcctgaagag aaagtgaacg
    15301 acgccgaggc caacgttcgc aagaccatcc gcctgacttc gcagatcgct tacgtggttg
    15361 ctgcctatga tcgcattcgc aaagggaggc atgtaattga gcctgaccgc gccctttcgc
    15421 acgccgcgaa ctttctctac cagttgaacg gcgaaatccc gagtgcgacc gccgagcggg
    15481 cgctcgacat tgctctcatc ctgcacgccg atcacgaact gaatgcctct accttcgccg
    15541 cacgcgtggt tgccgccacc ctcagcgaca
//
LOCUS       DASRKK010000235         7411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_114707, whole genome shotgun sequence.
ACCESSION   DASRKK010000235 DASRKK010000000
VERSION     DASRKK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114707"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFC15_05100"
     CDS             <1..480
                     /locus_tag="VFC15_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM09569.1"
                     /translation="VFDYGSVYVADRYWQNQGVLFAQDQVIRPTGITYDPYDKSLWLS
                     DGPSDIIAEYSLDGRLLYEFHTGLGGNSALAFDPADGTLWMINGGSLHECVFGRGACL
                     EQWSTTGQPLQFGNVPFLPISGFAAAEFPEPTPEPSSIMLLGTGILGLVGVLRRKLF"
     gene            complement(736..1848)
                     /gene="queA"
                     /locus_tag="VFC15_05105"
     CDS             complement(736..1848)
                     /gene="queA"
                     /locus_tag="VFC15_05105"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZM09570.1"
                     /translation="MLVSEFDYHLPDELIAKQPLANRAAARMLHVDRASDCLRDTTFR
                     EFPDLLRPDDLLVVNNTRVLPARLYGHRSGSRAQPISPSNPAAGQFLHGKVEVLLTRQ
                     LSTEPNVWEALVHPGRKLGIGERIYLGLDATEQSHELAAEIVARGEFGERHLRFDPVA
                     DFLSVLERIGHVPLPPYIDRPDTPLDREQYQTVYARPLGSVAAPTAGLHFTSEVLDRI
                     RARGIETAEVTLHVGLGTFQPIRVEVVEEHKIHREWYSISPEAAATIQRARDAAGRRI
                     VAVGTTTVRTLEAAARQSPDGRIVPQAAEAAIFIYPGFDFRVVGAILTNFHLPKSTLI
                     MLASAFAGRERTLSAYEHAVRGRYRFYSYGDCMLLE"
     gene            1965..2639
                     /locus_tag="VFC15_05110"
     CDS             1965..2639
                     /locus_tag="VFC15_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZM09571.1"
                     /translation="MKTEENYRDEIVRFGKLLHQTGLVAATDGNLSVRLGDGTFLCTP
                     TLMSKGLMEPGDMVIVDERGHKVSGRRNVSSEIAMHLFIYRRRPDVGAVVHAHPPTAT
                     GFAAAGIPLDSALCSEIVITLDKVPLANYGTPGTPELAEALAPLVGDHDAILMANHGV
                     VTYGEDLLNAYMNMETVEHYAKIALVTHMLGKQQPLSETHVSKLREIRQKYHAQNHVG
                     AARGDD"
     gene            complement(2711..4936)
                     /locus_tag="VFC15_05115"
     CDS             complement(2711..4936)
                     /locus_tag="VFC15_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM09572.1"
                     /translation="MTRIRFVVFCLLTSSALFAADLKLIVQSPSGERVSGVQVTLYRS
                     SDNAGVGILTTTGDGAVAFSNLADGAYQVVVLAPGFAAQSLQVALPQTEPLVVQLKLT
                     TTPQTVVVSATATPNTVNQTSVSLDLLNSAQLTLLNPPAAGDALSYIPGAIVNTSGRR
                     GSQASLFVRGGESSYNKVLIDGVPVNDPGGFYDFGVTPMNNIQRLEVVRGPESTIYGT
                     DAMTSVVQLWTTTGTTLKPVFEFGADGGTFSTANGYASVAGADKIFDYNFFLNQFDTQ
                     GQCINDSYTNGLQGGNVGIRFSQRVALRLRLRHSNNWTGVQSNWWFNGNPVLPPNSNQ
                     LAHQNNFLASAELAVSGPGAWQHTFSGYEYNHVQANSQATVDPGREFIDSPFSNLSKY
                     NRAGFEYQGQWTPRPWAQTTVGYTFEDENGFITSVFAPGTDYGSFSNTHGLRFNNYVF
                     AQELLTWKRVSALAGVGFVNNSSFGNKVVPRASVSFLLLQGNEVFSGTRLRAGYSEGI
                     KEPSFQQSFGISGTYPTLPNPNLQPEQNRSWEAGVEQGFFSNRLSLSALYYDNQFHNQ
                     IEYQTNPVDFTSQYVNFNKSMSHGAEVELRGQIASHLSFTGAYTYTSTQILEAPPCDP
                     PFCDPLIYGVGAPLLRRPKHAGTVLFTYVRPRFGATIGAVAVGRRPDSDFLFGFIPPI
                     YYAAGYARVDLGGWYNMTRHITAYANIGNALNDHYNEVLGYPALKANFRAGLRFRLGG
                     E"
     regulatory      complement(4961..5153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5231..5944)
                     /locus_tag="VFC15_05120"
     CDS             complement(5231..5944)
                     /locus_tag="VFC15_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM09573.1"
                     /translation="MPLESSDAVDDSGEHQFTLLQRVQLWLVSWIVPTFLYVIGPTLR
                     VTFSWEEGSIGSLDNIHPGIFPFWHRCVLGAAWIFRHRKLAVLTSRSLDGEFIARVIH
                     RMGYIPVRGSSSRGGQRGLLEMHTLVTNGGGVAFTIDGPRGPRYVAKRGPVLLARTTG
                     VPITAFYVAMEKVWVLNTWDAMMIPKPFSRASIRVARKIFVPPDADDTRLAEAHVEMQ
                     EALERITAYADSQFSSRRD"
     gene            complement(5989..7296)
                     /locus_tag="VFC15_05125"
     CDS             complement(5989..7296)
                     /locus_tag="VFC15_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09574.1"
                     /translation="MHNTWLIIRREYLERIRSKAFIIMTLLMPVFMASTILIPAMLSD
                     MKSGGTRRIVLVANNPEIAEAVKQQLMAPQQQTSETTDAKSDHVRSSEKVTAPRYAID
                     VSTTATDAERTSLRQQITDGKIDGFLWLTDNDLANRKVVYNARDVTDFGESIELRNAV
                     QSAVVKRQLAQKGMSGVEVETLLKPIDLDSIRIEKGKEGASGISVFLVSFTMVMLLYV
                     NVLVYGVAVMRSIIEEKSSRILEVLLSTVTAKQLLAGKIIGVGAVGLTQIIIWVAVAG
                     AFSVPGLMASRSMLSNIHIPMAGVIAFGVFFILGYFLYSTMYAALGAMVNSDQEAQQV
                     QWPAMLPIIFSIVLSTPVLQHPNSPLAFWTSMVPFFAPILMFVRVMVETPPMWQIVLC
                     IALMLLTTWGMLSLSSRIYRVGILMYGKRPTLPELRRWLKYSG"
BASE COUNT         1567 a         2074 c         2303 g         1467 t
ORIGIN      
        1 gttttcgact atggaagtgt ttatgtagct gatcgttact ggcagaacca gggcgtgcta
       61 tttgcccaag accaagtcat acgcccgacc ggaataactt atgatccgta tgacaagtcg
      121 ctctggctca gcgacggtcc aagtgacatc attgccgaat actctctaga cggccggctc
      181 ttgtatgaat tccatactgg actgggcggc aattctgccc tcgctttcga cccggctgat
      241 ggcacgctct ggatgatcaa cggtggcagc ctacatgaat gtgtttttgg ccgaggggcg
      301 tgtttggagc agtggtcaac caccggacag ccactccagt ttggaaacgt cccgtttttg
      361 cccatttcag gtttcgctgc ggctgagttc ccagaaccaa cccccgaacc cagcagcatc
      421 atgctgttag gcacgggtat ccttggcttg gttggcgtac tgcggcggaa gctattctag
      481 ccaatcgcag cgcaccaggc gtaattgagc cggaatattc gctccggctc cttttcttcc
      541 tggctgacca cgtgaaatcc ggtgcggagc aacttcggcc tgtactataa gcagatgagc
      601 tcgaactaga cctcagaggc tggagactgg tgcatgatgg cgagactcgg cagacgttat
      661 ttcgttccct cttacgagga atctcggctt acggcaagcg gctaaagagc cgagaactga
      721 cgactccttt cctttctact ccagcaacat gcaatccccg tacgagtaga atcggtacct
      781 cccgcggacg gcgtgttcgt acgctgacag tgtgcgttcg cggccggcaa aggccgacgc
      841 cagcatgatc agcgtggact tgggcagatg gaagttggtc agtatggctc ccaccacgcg
      901 aaagtcgaat cccggataaa tgaagatggc agcctcagcc gcttgcggaa cgattcggcc
      961 gtcgggcgac tgacgcgctg cggcttccag ggtgcggacg gtggtggtgc caacggcaac
     1021 gatcctgcgt ccagcagcat cgcgggcgcg ctggatggtt gccgcggctt ctggcgaaat
     1081 cgaataccac tcgcgatgga tcttatgctc ttcgactact tcgacgcgga tgggctggaa
     1141 ggtaccgagc ccgacatgca gtgtgacctc ggcagtctcg atgccacggg cgcgaatgcg
     1201 gtcaagcacc tcggacgtga agtgcaatcc ggcggtggga gcggcgaccg agcccagcgg
     1261 ccgggcatag acggtctggt actgctcgcg atcgagcggc gtgtcgggac gatcgatgta
     1321 ggggggaagc gggacgtgtc cgatgcgctc gagcacactg aggaaatcag cgacgggatc
     1381 gaagcgcaga tggcgttcgc caaattcgcc gcgggcgacg atttcagccg caagttcgtg
     1441 cgactgctca gtggcgtcga gaccaagata gatgcgctcg ccgatgccca gcttgcgtcc
     1501 gggatggacg agcgcttccc acacattagg ctcggtggag agctggcgcg tcagcagcac
     1561 ttcgaccttg ccgtgcagga attgaccggc tgcggggttg gacggggaaa tgggctgcgc
     1621 tcgcgaaccg ctgcgatgtc cgtagagccg cgccggcaac acgcgcgtat tgttaaccac
     1681 cagcaaatcg tcagggcgga gcaggtcagg gaactcgcgg aaggtggtgt cacgcaagca
     1741 gtcacttgca cgatcgacgt gcaacatgcg agcggcggcc cggttggcga ggggctgttt
     1801 cgcgatcaac tcgtctggaa ggtggtagtc gaactcagaa accagcacaa tttgattatg
     1861 ccctacagaa tttcacaaaa tggtcacctt cgggcttttg cggaattcga aaactaggtt
     1921 acgataaaga cactagggat gtgtacctcc agcagtaccg cagcatgaag acagaagaaa
     1981 attatcggga cgagatcgtt cgcttcggca agttgctgca tcagaccgga ctggtggctg
     2041 cgacggatgg caacctttcg gtgcgcctgg gcgacggcac ctttctatgt actcccacgc
     2101 tgatgagcaa gggtctaatg gagccgggcg acatggtcat cgtggatgag cgcgggcata
     2161 aggtcagcgg ccggcgcaat gtttcgagcg aaatcgcgat gcacctgttc atctaccgca
     2221 ggcggcctga cgtgggagcg gtggtgcacg cgcatccccc caccgccacc ggattcgccg
     2281 ccgccggaat tccgctggac agcgcgctgt gctccgaaat cgtgattacg ctggacaaag
     2341 ttcccttggc gaactatgga acgccgggta cgccggagct ggcggaggcg ctcgccccgc
     2401 tggtcggcga tcatgatgcc atcctcatgg cgaaccacgg tgtcgtgacc tacggcgaag
     2461 acctgcttaa cgcctacatg aacatggaaa cggtggagca ctatgccaag atcgcgctgg
     2521 tcacacacat gctgggcaag cagcagccgc tgagcgagac acacgtgagc aagctgcgcg
     2581 agatccgcca gaagtatcac gcccagaatc acgttggcgc ggcgaggggc gacgactaag
     2641 gaagcggcta gtggctggtg gtcagaggct ggtgaaatca gtttgagttt gttcgtgtct
     2701 taaatccgcg ttattccccg cccagccgga agcgtaaacc ggcacggaag tttgccttca
     2761 gcgcgggata tcccaacact tcgttgtaat gatcgtttag cgcgttgccg atattcgcat
     2821 acgcggtgat gtggcgcgtc atgttgtacc atccgccgag atcgacgcgc gcgtatcccg
     2881 cggcgtaata gatgggaggg atgaatccga agagaaagtc ggagtcggga cggcgaccca
     2941 ccgccaccgc gccaatcgta gcgccgaagc gcgggcgcac gtaggtgaac agaaccgttc
     3001 cggcatgttt tgggcggcgg agcaacggcg cgcccacgcc atagatgagc ggatcgcaaa
     3061 acggcgggtc gcaaggcggc gcttctaata tctgagtcga ggtgtaggta taggcgccgg
     3121 tgaaggagag gtggctggcg atctggccgc gcaactcgac ttcggccccg tgcgacatcg
     3181 acttgttgaa gttgacgtac tggctggtga aatccacggg gttggtttga tactcgatct
     3241 gattgtgaaa ctggttgtcg tagtacaacg cgctcagcga tagccggtta ctgaaaaatc
     3301 cctgctcgac acccgcttcc cacgaacggt tctgctcggg ctgcagatta ggatttggca
     3361 gcgtgggata ggtcccggat attccaaatg actgctggaa actcggctct ttgatgccct
     3421 cggagtatcc ggcgcgcaag cgggtgccgc tgaatacttc attcccttgc aacaaaagaa
     3481 atgagaccga agcgcgcggc acaactttgt tgccgaagct ggagttgttg acgaacccga
     3541 ccccagcgag cgcggatacg cgtttccaag tcagcagttc ctgtgcaaat acatagttgt
     3601 tgaagcgcag cccgtgagtg ttgctgaacg atccgtaatc ggtgccaggt gcaaagacag
     3661 acgtaatgaa tccgttctcg tcttcgaagg tgtagccgac ggtggtttgc gcccaggggc
     3721 ggggcgtcca ttgcccctgg tattcgaagc cggcgcgatt gtatttgctg aggttcgaaa
     3781 acgggctgtc gatgaactcg cgccccggat cgaccgtggc ctgcgaattc gcctgcacgt
     3841 ggttgtactc gtaaccgctg aaggtgtgct gccaggctcc ggggccgctg acggcaagct
     3901 cggcgctggc caggaaattg ttctggtgcg cgagttgatt ggagttgggc ggcagcacgg
     3961 gattgccatt gaaccaccag ttcgactgca cacccgtcca gttgttcgag tggcgaagcc
     4021 gcagtcgaag ggcaactcgc tgcgaaaagc gaatgccgac gttgccgcct tgcagtccgt
     4081 tggtatagct gtcgttgatg cattgtccct gggtgtcgaa ctggttgagg aagaagttgt
     4141 agtcgaaaat cttgtccgca cctgcgaccg atgcgtatcc gttggcggtg gagaaggtgc
     4201 cgccatcggc gccgaattcg aagaccggct tcaaggtcgt tccagtcgtg gtccagagtt
     4261 gcaccacgct ggtcatggcg tcggtgccgt aaatggtgct ctcgggtcca cgcacgactt
     4321 ccaggcgctg aatgttgttc atgggagtca cgccgaaatc gtagaagccg ccgggatcgt
     4381 tgaccggcac tccgtcgatg agcactttgt tatagctgga ttcgccgccg cgcacgaaca
     4441 ggctggcctg gctgccgcgg cggccgctgg tgttgacgat ggctccggga atgtagctga
     4501 gagcgtcgcc agctgcaggc ggattgagca gagtgagctg agcgctgttg agcagatcga
     4561 gcgacaccga agtctgattg acggtgttgg gcgtagcggt ggcggaaacc acaacggtct
     4621 gcggcgtggt tgtcagcttc agttgcacga ccagcggttc agtctgcgga agcgcaacct
     4681 gcagtgactg cgccgcgaat ccgggagcca gcaccacaac ttgataagca ccgtcggcca
     4741 ggttcgaaaa cgcgacagca ccatcgccgg tagtcgtgag gatgcctact cccgcgttgt
     4801 cgctggagcg atacagcgtc acctgtactc cggacacacg ttcgccggag gggctttgca
     4861 cgatgagttt caggtcggca gcgaacagcg cgctggaagt cagcaagcag aaaacaacga
     4921 atcgaatgcg ggtcaacact cctcctcacg cgaaaggggt tggatggatg aagattcgcg
     4981 ccggtctcct gacttgcgag ttgcgatgcg gacatcgcga acaacggcgc aggccttccc
     5041 gtttgcacag tggcctagcg ccaaacagct cgcttacagt tgcgcggcag cgcgggattt
     5101 acacccgctt cccagttccg cccagtggcg gaacgcgcga accaaaatcg tggatgatct
     5161 ggaaagaacg acctgcggcc gaaatttgcc ccaggagaaa ctctgacttt atcggccagc
     5221 aggaccagtg tcaatcgcga cgcgaagaaa actgcgagtc agcataggcg gtgatgcgtt
     5281 ccagtgcttc ctgcatctcg acgtgtgctt ccgccagtcg agtgtcatcg gcgtcgggag
     5341 gaacgaagat cttgcgggca acgcggatgg aggcacgcga aaaaggcttg gggatcatca
     5401 tcgcgtccca ggtgttgagc acccagactt tttccatcgc cacataaaac gccgtgatgg
     5461 ggacccccgt agtgcgcgca agaagtaccg gtcctcgttt ggcgacgtag cgcggaccgc
     5521 gagggccgtc gatggtgaac gctacgccgc ccccgttcgt gaccagcgtg tgcatctcga
     5581 gcagtccacg ctgacctccg cgcgaactgg agccgcgcac cggtatgtat cccatgcgat
     5641 ggatcacgcg tgcaatgaat tcgccatcga gactgcggct ggtcagcaca gccagtttgc
     5701 gatggcgaaa aatccatgcg gcgcccagca cgcagcgatg ccagaacgga aagattccgg
     5761 gatgaatatt atccaagctg ccaatcgagc cttcttccca agagaacgtc acccgcagtg
     5821 tcggcccgat cacgtagaga aatgtcggga cgatccacga aacgagccag agttgcactc
     5881 gctgcagcag cgtgaattgg tgttcgccgg aatcgtcaac ggcgtcgctg gattcgaggg
     5941 gcacgcgaga attctaaatc agtgaccact aaccactcgc ttcacgcttc accccgagta
     6001 cttcagccac cgccgcagtt ccgggagcgt cggtcgcttg ccatacatca ggatccccac
     6061 gcgatagatg cgcgaagaca gactgagcat tccccacgtc gtgagcagca tcagcgcgat
     6121 gcagagaacg atctgccaca tgggaggcgt ctcgaccatc acgcggacga acatgagaat
     6181 tggcgcgaag aagggcacca tcgatgtcca gaacgccaac ggtgagttgg gatgttgcag
     6241 cacaggcgtg ctcagcacga tggagaagat gatgggcagc atggcgggcc actgcacctg
     6301 ctgcgcttcc tggtcggagt tgaccatcgc gcccagcgcg gcatacatcg tcgaatagag
     6361 gaagtagccg agaatgaaaa aaactccgaa agcaatcacg cccgccatcg gaatatggat
     6421 gttgctgagc atggagcgcg aggccatcag tccgggaacg gagaaagctc cggcaaccgc
     6481 cacccagatg atgatttgag tgaggccgac cgcgccaacg ccgatgatct ttcccgccag
     6541 tagttgcttc gcggtcacgg tggagagcag cacttcgaga atgcgcgagc ttttctcctc
     6601 gatgatggag cgcatcacgg cgacgccgta caccagcacg ttcacataga gcaacatgac
     6661 catggtgaac gaaaccagga ataccgaaat gccgctggcg ccctccttgc ccttctcgat
     6721 gcggatggag tctaggtcga ttggcttgag cagggtctcc acctccacgc cgctcatgcc
     6781 tttttgcgcc aactggcgct tgaccacagc cgactgcacg gcgttgcgca attcgatgct
     6841 ttcaccaaag tcggtcacat ccctggcgtt gtaaaccacc ttgcgattgg caaggtcgtt
     6901 gtccgtgagc cagagaaagc catcgatctt gccgtcggta atctgctgcc gcagggaagt
     6961 gcgctcggcg tccgtggcag tagtgctgac gtcaatggcg tagcgcgggg ccgttacttt
     7021 ctcgctcgac ctaacgtgat cgctcttcgc atcagtcgtc tcgctggtct gctgttgtgg
     7081 agccatcaac tgctgcttca ctgcctcggc gatctcggga ttgttagcca ctagcacgat
     7141 gcgccgcgtg cccccggact tcatgtcgga aagcatggcc ggtatcagga tcgtggatgc
     7201 catgaacacc ggcatcagca gcgtcatgat gatgaacgcc ttgctgcgga tgcgctccag
     7261 atattcgcgc cggatgatca gccaggtgtt atgcattttg gcccaccact tcaatgaaga
     7321 tttcttccag cgatggctca accagctcga aacgggtgat gcgagcgtgc tgcgcgcaca
     7381 ggtgcagtaa ttcctgcgaa tcggcgccgg g
//
LOCUS       DASRKK010000236        13873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_506111, whole genome shotgun sequence.
ACCESSION   DASRKK010000236 DASRKK010000000
VERSION     DASRKK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506111"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFC15_05130"
     CDS             complement(<1..365)
                     /locus_tag="VFC15_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09575.1"
                     /translation="MGCSSFSGGINLVSSRFDHDSCGVGFVATLHGQSDHEILRLALT
                     ALGRLAHRGAIAADGKSSDGVGVQTAVPRSFLLSQTGITLRPEQPLAVGMLFLPQNEE
                     AAQRDGQRFKACLSARGFE"
     gene            480..1748
                     /locus_tag="VFC15_05135"
     CDS             480..1748
                     /locus_tag="VFC15_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM09576.1"
                     /translation="MADGQNLQRQGRVAFTYPDFTYYQLARLFTVMATEMQSVAVGWQ
                     VYEITKRPLDLGYVGLAQFLPTILLFLVAGHAADRFHRRNIVTVCYAGLGLASTILFL
                     LTISGSRSVHAIYAALVLVGVVRSFNGAAGQSMVPQLVPEEHFPNAVAWAQTVFNAAV
                     IIGPALGGLIYAVSRGPAAVYASSMIASAISVVAVSRIRLNPAKREREPVSLSTLFAG
                     LRFIWQKKLILGTISLDLFAVLLGGAVALLPVYAREILHIGPWGLGILRSAPAVGAVS
                     MAVLLAHRPLRRHAGITMLWCVAGFGVFTIVFGLSRNLFVSLLALLLLGASDMVSVII
                     RGTLVQLSTPDSMRGRVSAVNSLFIGTSNEFGQFESGLTAQWFGTVPAVVIGGVGTLL
                     VIALWSWLIPELRGADTLTTRPSTVAEESP"
     gene            1745..2632
                     /locus_tag="VFC15_05140"
     CDS             1745..2632
                     /locus_tag="VFC15_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09577.1"
                     /translation="MTAGSAYYASQLRHELSLRNRAFALQRRLAHVESFGGMAVTVYQ
                     PYPTRRRHGNFFDESYAAMLAIPEWRKRLEKVHSQAKSSLPKTGRGWRELDSSMSSDA
                     LLMNIFCCPYVPDDPRVLGFLGIEAAELPQFGWRARVPLKTGLTDRTEVDMKLGNMLF
                     EAKLTESDFQSRNSAVLRGYRDFQEVFDSKSLPREGEKLVSYQLIRNVLAAHASDCGF
                     CVLLDARRPDLLESWYAILRGIRPAELRVRCKVLTWQELSVVLPEQLCEFLDVKYGIV
                     APGKVPSIVFNGKDLEAEE"
     gene            3067..4821
                     /gene="ilvD"
                     /locus_tag="VFC15_05145"
     CDS             3067..4821
                     /gene="ilvD"
                     /locus_tag="VFC15_05145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZM09578.1"
                     /translation="MSDDSCRERSRHIIEGPNRAPARAMFKAVGFTDDDLRKPIIGIA
                     NTWTEVGPCNYHLREIAAAVKEGVREAGGTPMEFNTIAVSDGITMGSDGMRASLVSRE
                     VIADSIELVARGHMFDGVVALSSCDKTIPGTVMALARLDIPSLMLYGGSIAPGHYQGR
                     DVSIQDVFEAVGKYAQGKMNDAELKDMEDHACPGPGACGGQFTANTMSTACEFLGISP
                     LGSNAVPATSPLKYEVAKKAGHLVMELLRRDIRPSQIMTDAALKNAMTCVVASGGSTN
                     AVLHLPAIANELGLSFSLDEINHICSRTPVLCDLKPGGRFMATDFFRAGGTKLLGHIL
                     AEAGLINGECITVSSKTLGEEVAQATEQPGQEVIRPVERALSPTGGLAILRGNLSPEG
                     CVVKLVGQARREHRGPARVFESEELAFEAVQSSQIKAGDVIVIRNEGPRGGPGMREML
                     AVTAALAATPLSEHVALLTDGRFSGATHGFMVAHIAPEAAVGGPIAAVHEGDMITIDV
                     DHGRLQLEVSNAELGSRMTKWVPPVPRYKTGVFAKYANSVSSAALGAVTTPGRGREDA
                     AADGIDVPDFAKAGALGR"
     gene            4818..6539
                     /gene="ilvB"
                     /locus_tag="VFC15_05150"
     CDS             4818..6539
                     /gene="ilvB"
                     /locus_tag="VFC15_05150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZM09579.1"
                     /translation="MSKMRLSGAQIVWKCLEREGVDTVFGYPGGCVLPLYDALQHTPS
                     IHHVLVRHEQGAAHMADGYSRATGKVGVALATSGPGATNLVTGIATAMLDSSPIVCIT
                     GQVGSKFIGTDAFQEIDITGITMPITKHNYMVTDVNDIARTLREAFIIARTARPGPVL
                     VDITKDAQMASCDFEWDFVMPRLRKLETWEAPDPQQLAEAAALINKAERPVILAGHGI
                     LMSGAMQDLQLFAEASDIPVGLTLLGLGAMPHFHPLCLGMMGMHGEAWLNHAIQNADL
                     LIALGMRFDDRVTGKLDSYAQNAKKIHVDIDASEIHKNVRADVAIVGDVKLVLPKLTP
                     LLTPMKRSTYWAEINNAKGAVAVRDIRNLPDTGHLYAAHVIHDLWRETKGKALIVTDV
                     GQHQMWEAQYYHHSEPRSLITSGGLGTMGFALPAAIGAKFSRPDADVWVIVGDGGFQM
                     TMAELATMVQENIKVNIAIINNGYLGMVRQWQEFFYERNYAATPLVNPDFTKIAEAYG
                     ILTFAVTHRDEVAPTVQKAREHPGPVLIDFRVEQEDSVYPMVPAGKALHEMIRRPSAI
                     VETATDE"
     gene            6544..7152
                     /gene="ilvN"
                     /locus_tag="VFC15_05155"
     CDS             6544..7152
                     /gene="ilvN"
                     /locus_tag="VFC15_05155"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZM09580.1"
                     /translation="MNMTYLLDVANHTGVVTRISLIFRRHGANLQSLSTAKTEHPSIV
                     RIVITSDLEGRAAHRIKGQLSKLIDIFKVDMISNEEGTFRAFGMMKIACTLQTRPQIL
                     QLLEIFNGRVLELTTEWILVEMTGTPEQLEGLLQVLQPYGILEAVETGPIVIRRNSPR
                     NGTRPQPTWEVVPGALSAATNSTTTSTSNSTQERKRNGTDLL"
     gene            7133..8185
                     /gene="ilvC"
                     /locus_tag="VFC15_05160"
     CDS             7133..8185
                     /gene="ilvC"
                     /locus_tag="VFC15_05160"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZM09581.1"
                     /translation="MAQIFYDKDADLSLVKATKVAIIGYGSQGHAHALNLRDSGVDVC
                     IGLHPGSRSRARAEAAGLTVKSVSDAAAWADTIMVLAPDTEQAKIYHRDIQPNLKPGK
                     TLMFAHGFNIRFGTIKCPAGVDVSMIAPKAPGHRVREVFVEGGGTPCLVAVEQDASGR
                     AWQYALSYAKGIGGTRAGVLKTTFTEETETDLFGEQAVLCGGASALVKAGFETLVDAG
                     YQPEVAYFECLHELKLIVDLMYRGGLSYMRYSVSDTAEHGDYTAGKRLINDETRKEMK
                     QILEEIRNGKFAAAWIKENETGRHNFIAERERQRDHQIESVGERLRDMMPFLNPVKVS
                     DIEAPAKKEEPVAAAR"
     gene            8299..9252
                     /locus_tag="VFC15_05165"
     CDS             8299..9252
                     /locus_tag="VFC15_05165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZM09582.1"
                     /translation="MAFFSESKWVWKNGKIIPWEQATVHMSAHGLHYGTGVFEGVRCY
                     NTPDGPAVFRLEAHLQRMFESARTYGLPIPYSVEELTQGAIDVIKANGFSDCYVRPIA
                     TYGSASLGVHPRNCPVEVAMLAWPWGAYLGADALENGIQVTVSRWKKFSSLAMPATAK
                     ACGQYLNSMLAVQDAVRCGFDEALLVDEEGVIAEGSGENFFMVQNGNLLTNDERDHVL
                     MGITRDSVITLARDLGYKAFVGQMRLEELEVADEAFFTGTAAEVTPISALDRKPIGNG
                     KPGPVTLELQRAYFAATSGKDPKHRDWLTQVNAKSEVKESV"
     gene            complement(9256..10176)
                     /locus_tag="VFC15_05170"
     CDS             complement(9256..10176)
                     /locus_tag="VFC15_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZM09583.1"
                     /translation="MMELFQLETFLAVAEEKSFSRAAARMHRTQSAVSQTISKLEDEL
                     GERLFDRSSRDGSLTDAGQVLQTYAKELLNVREEARESLKELRMLHQGKLVIAANEFT
                     SRYLLPVLHQFHRTHPMIRIQVMRTLAREVPGQVLSHQVEFGVVSFRPEDGELTSHVL
                     YRDELALIVYPRHPLAHAKHVGIKDLGAESFIAHSVLSPYREKVIQAFKKHHTPLHMN
                     VELPNIADIKQFVRMENGVALVPLIAVEHEIARGDLIHVPVPELAMERKLRLVYRTGT
                     VLSHAGQAFLKTCKAVSQAPGSRYLMLSEH"
     gene            10306..11508
                     /locus_tag="VFC15_05175"
     CDS             10306..11508
                     /locus_tag="VFC15_05175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZM09584.1"
                     /translation="MQRDRVLVFDTSLRDGEQAPGCSMNGDEKLLFAKKLQVLGVDII
                     EAGFPVASAGDTEAVRRIATEVQGPVIAALARCVEKDIAVAWEAVSRSMRPRIHTFLA
                     TSDLHLAFKLKIGRDEALNQARKMVAYARSLCTDVEFSPEDATRSDVGFLCEVLQAAV
                     DSGASTLNIPDTVGYTTPGEYGELIRTIRQRVKGIENVTISVHCHNDLGLAVANSLAG
                     VEAGARQVECTVNGIGERAGNASLEEFAMALHVRADRYGIDTGINHAQIYSTSQLLSD
                     LIGFEVQPNKAIVGRNAFAHEAGIHQHGVLNNPLCYEIMTPETVGIPAERIVLGKHSG
                     RHALVFKYRELGHELSGDELNRIYQEFTRLADRKKVIYEQDLLAMLCTHRSGMREHTS
                     PSPEMRPA"
     gene            11505..12608
                     /gene="leuB"
                     /locus_tag="VFC15_05180"
     CDS             11505..12608
                     /gene="leuB"
                     /locus_tag="VFC15_05180"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZM09585.1"
                     /translation="MKLRIVVLPGDGIGPEVTRQAVAVLQTVSDVCGYEFRFESHRIG
                     GAAIEQDGKPLPDKTLAACLESDAVLLGAVGARKFDELTGEKRPEAGLLALRQALGGF
                     ANLRPVKAYEAIADCSPLREDVTRGADILIVRELLGGLYFAQPRALNEDGLSAFNTMR
                     YHVDEVERVAHVAFQEARRRKHLVTSVDKANVLETSQLWRRTVTALAKEYPDVQLEHA
                     YVDSFAMQVITTPARYDVVLTENLFGDILSDEAAVISGSLGMLASASVGGKVELYEPV
                     HGSAPDIAGKDLANPFGAIQSAAMLLRYTAGLYHESDKIMQAVDRTLARGLVTQDLSG
                     RRNRPAVPTDAIGQAVVETVTDLLQMQMAYHAV"
     gene            12616..>13873
                     /gene="leuC"
                     /locus_tag="VFC15_05185"
     CDS             12616..>13873
                     /gene="leuC"
                     /locus_tag="VFC15_05185"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZM09586.1"
                     /translation="MYKPKSLFEKVWDSHVVRAPEGEPTLLYIDLHLVHEVTTPQAFE
                     GLRLANRKVRRPDLTFATIDHNVPTTSDERFHIKDQVAAQQIATLRQNCEAAGIPLFD
                     IGSGRQGIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQC
                     VSQTKPRSMRISVSRRLPEGTTSKDLILDIIRRLGTDGGTGHVIEYAGEAVRGLTMEE
                     RMTMCNMSIECGARAGMVAPDEITFAYMEGRPYAPKGSAWNKAVTAWRQLPSDEGATF
                     DREISIDGSKLEPIVTWGTNPGMSVPVTANVPNPEDARDASERAAMIAALEYMGLQAG
                     TRVEDIAVDRIFIGSCTNGRLEDLRAAAKVIHGHKVHPDVTVLVVPGSEAVKLAAEHE
                     GLDRIFKEAGAQWREPGCSMCLGMNPD"
BASE COUNT         2904 a         3923 c         4287 g         2759 t
ORIGIN      
        1 cactcaaagc cgcgtgccga gagacatgct ttgaatcgct gcccatcacg ctgggcggct
       61 tcttcattct gcggtagaaa taacattccg accgccagcg gttgctcagg gcgcagcgtg
      121 atgccggttt gactgagcag aaaactgcga ggcacggcgg tttgcacgcc cacgccatca
      181 ctgcttttcc cgtcggcggc aattgcgccg cgatgggcga ggcggcctag agcggtcaat
      241 gccaggcgaa ggatttcgtg gtcagactgg ccgtggaggg tcgcgacaaa cccaactccg
      301 caggagtcat ggtcgaagcg agagcttacg aggttaatgc caccagaaaa actgctacaa
      361 cccatagtcc actatatatg gaacttgccg tgcaaatgaa gtgggaattc cgacgacgaa
      421 gaaaaagttt ttcccatttt gagatcgcgc acgtcgtacc tgtggcacag tagtgtggga
      481 tggctgatgg ccaaaacttg caacgccaag ggcgagtagc gtttacctat cctgacttta
      541 cctactacca actggcgcgg ctgttcactg tgatggcgac ggagatgcaa tcagtcgcgg
      601 tgggctggca ggtttacgaa atcaccaagc ggcccttgga tctcggctac gtcggcttgg
      661 cgcaatttct tcctaccatc ctgctatttc tggttgccgg acacgctgcc gaccgcttcc
      721 accggcgaaa tattgtcacc gtctgttatg ccgggcttgg cctggcttca acaatcctgt
      781 tcctgttgac gataagcgga tcgcgctcag ttcacgcgat ctatgcggca ttggtgctgg
      841 tgggagtggt gcgctcgttc aacggtgccg cgggacagtc gatggttcca caactggtgc
      901 ccgaggaaca tttccccaat gccgtggctt gggcgcagac agtgtttaat gcagctgtga
      961 tcatcggtcc agcgcttggc gggttgatct acgcggtttc gagggggcct gcggctgtct
     1021 atgccagttc catgatcgct tctgcaatat cggtggttgc ggtcagtcgg atacgattga
     1081 atcctgccaa gcgcgagcgc gaaccggtga gtttgtccac tctatttgcc ggccttcggt
     1141 tcatctggca aaagaagctc atcctgggca cgatctcgct cgatctgttt gcggtactgc
     1201 ttggtggagc cgtagcgctg ctgccggtct acgcacgcga gatactgcat atcgggcctt
     1261 ggggactcgg tattctgcgc agcgcaccag cagttggggc tgtgtcaatg gcagtcctgc
     1321 tggcgcatcg gccattgcgc cggcacgctg gtatcaccat gctgtggtgc gttgctggat
     1381 tcggcgtatt taccatcgtg ttcgggcttt cgcgcaatct ctttgtctcg ctactggcgc
     1441 ttttgcttct gggcgcctcc gacatggtga gtgtcatcat tcgcggaacg ctggtacagt
     1501 tgtccacccc ggactccatg cgtgggcgcg tcagtgcggt gaattctttg ttcattggga
     1561 cctcgaacga gttcgggcaa ttcgagtcgg gattgacagc gcaatggttc ggcacagttc
     1621 cggcggtggt aatcggcggt gtcggaacac tactcgtgat cgccctctgg tcgtggttga
     1681 ttcccgaatt gcgcggcgcc gatactctta ccactcgacc atcaaccgtc gcagaggaat
     1741 cgccatgacg gcgggcagcg cctattacgc ctcacaacta cgccacgagc tgagcctgcg
     1801 caaccgcgca ttcgcgctgc agcgaaggct cgcacacgtt gagagcttcg gcggaatggc
     1861 ggtaaccgtg tatcaaccgt acccgacccg ccgccggcat ggcaacttct tcgatgaaag
     1921 ttacgccgcc atgctggcta ttccggaatg gcgcaagcgg ctggagaaag tgcattcaca
     1981 agccaagtcg tcgttgccca agaccggccg aggctggagg gaactggact cgtcgatgag
     2041 ttcggacgcg ttgctgatga acattttctg ttgtccgtat gtcccggatg acccgcgcgt
     2101 gctgggcttt ctgggaatag aggctgccga gttgccgcaa ttcggatggc gggcccgcgt
     2161 gccactgaag acaggtttga ccgaccgcac cgaggtcgac atgaaattgg ggaacatgct
     2221 gttcgaagcc aagctcaccg agtcggattt ccagtcccgc aactcggcgg tgttgcgcgg
     2281 ctatcgcgac ttccaggagg ttttcgactc caagagtctt ccgcgcgaag gggaaaagtt
     2341 ggtttcgtat caactgattc gcaacgtgct cgccgcgcac gccagcgatt gcgggttctg
     2401 tgttttgctc gacgcacgtc ggcccgattt gctggaaagt tggtatgcga ttctccgcgg
     2461 catccgcccc gccgagctgc gggtgcgctg caaggtattg acctggcagg agttaagcgt
     2521 ggtcttacca gagcaactgt gcgagttcct tgacgtcaag tacgggatcg tggcgcccgg
     2581 gaaagttccg tctatagtat tcaacggaaa ggacttggag gccgaggaat aaccgggttg
     2641 gcgggttgcg ggcatgcgct gccccggagt cggcagccca ttagtgtctt ttataaattt
     2701 tggtacatca ttaacaaaac ttatgatcac gtaaaatttt ttaatttctc tcgacttacc
     2761 tcgatcaatt taggtataaa tcacaaatcg cttatgacat cggcagcgca atctcgagtc
     2821 caaccgatta gcggctcgat cctcgcgatc cggcccatgg ccctagtccg tgtgctttcg
     2881 cccgcactac ttctacttgg agtagcgggc tacataggag gcgcggggac gtaggcaaac
     2941 agagaaaaag cagattctga aaagccactg acccgctgag cggaagtggc ttttgtgcta
     3001 tagagcacaa cataacgccg tgacgtttac gtttgaggat cgaactttga ggatagaaga
     3061 acaacgatga gcgacgacag ttgtagagag cgcagccggc acatcatcga agggcccaac
     3121 cgggctcccg cgcgcgccat gttcaaggcc gttggcttca ccgacgacga cctccgcaaa
     3181 cccatcatcg gaatcgccaa tacctggacg gaagtcggcc cctgcaacta tcacttgcgt
     3241 gaaatcgctg ccgccgtgaa agaaggcgtg cgcgaggccg gcggcacacc gatggagttc
     3301 aatactatcg cggtttccga cggaatcacc atggggtcgg acggaatgcg cgcctcgctg
     3361 gtcagccgcg aggtgattgc cgactcgatc gaactggtag cgcgcggaca catgttcgat
     3421 ggcgtggtcg cactgtcgag ttgcgacaag accatccccg gcacggtgat ggcgctggca
     3481 aggctcgata ttccctcgtt gatgctctat ggcggatcga tcgcgcccgg ccactaccag
     3541 ggacgcgacg tcagcatcca ggatgtgttc gaggctgtag gcaaatacgc gcagggcaag
     3601 atgaacgacg ccgagctgaa ggacatggag gaccatgcat gtccaggccc cggcgcctgt
     3661 ggcggacagt tcaccgcaaa caccatgtca actgcctgcg aattcctcgg tatctccccg
     3721 ctcggtagta acgcagttcc cgcaacctct cccctgaaat acgaagtcgc gaagaaagcc
     3781 gggcacctgg tcatggagtt gttgcggcgc gatattcgtc cttcgcagat catgaccgat
     3841 gcggcgctga agaacgccat gacgtgcgtt gtggcttccg gcggctcgac caacgccgtc
     3901 ctgcatctgc ccgccatcgc caacgaactg ggactcagtt tttcgctcga cgaaatcaac
     3961 cacatctgct ctcgcactcc agtgctgtgc gatctgaagc cgggtggccg cttcatggcg
     4021 accgattttt tccgcgccgg aggcaccaag ttgctgggac acatcctggc ggaagccggt
     4081 ttgatcaacg gcgaatgcat aacggtgagt agtaaaacgc tgggcgaaga ggtcgcgcaa
     4141 gcgaccgagc agccgggaca ggaagtgatt cgtccggtgg agcgcgcgct gagtccgacg
     4201 ggcggcttgg cgattctacg cggcaatctt tctcccgaag gatgcgtggt caaactggtc
     4261 ggtcaggccc ggcgcgagca tcgcggtccg gcgcgggtct tcgaatcgga agagctggcg
     4321 ttcgaagcag tgcagagttc gcaaatcaag gctggcgatg taatcgtgat tcgcaacgaa
     4381 gggccgcgcg gtggaccggg tatgcgcgag atgttggccg tgaccgcggc cttggcggcg
     4441 acgccgctga gcgaacatgt ggccttgctg accgatggcc gcttctccgg cgcaactcat
     4501 ggcttcatgg ttgcgcacat tgctccggaa gccgcagtgg gtgggcctat cgcggcggtg
     4561 catgagggag acatgattac gatcgacgtt gatcatggca gattgcagct cgaagtctcc
     4621 aatgcggagt tggggagcag gatgaccaag tgggtgccac cagtcccacg atacaagacc
     4681 ggagttttcg ccaagtacgc gaacagcgtt tcgtcggccg cgttgggtgc ggtcaccacg
     4741 ccgggcaggg gacgcgaaga cgccgcggca gacgggatcg atgttcccga tttcgccaag
     4801 gcgggagcac tggggagatg agcaaaatgc gattgagcgg tgcgcaaatc gtttggaagt
     4861 gcctggagcg tgaaggcgtg gacactgtct tcggctatcc cggcggatgc gtgctgccgc
     4921 tgtacgacgc gctgcaacac actcccagca ttcaccacgt gctggtgcgt catgagcagg
     4981 gcgcagccca catggccgac ggatactccc gcgccaccgg aaaggtcggt gtagcgttgg
     5041 ccacctcagg tcccggagcg accaacctgg ttaccggcat cgcgactgcc atgctcgact
     5101 cctcgcccat cgtatgcata accgggcagg tgggcagcaa gtttatcggc accgatgcct
     5161 tccaggaaat cgacatcacc ggcatcacca tgccgatcac caagcacaac tacatggtga
     5221 cggacgtcaa tgacattgcg cggacgctgc gcgaagcgtt catcatcgcg cgcacggcac
     5281 gccccggccc ggtgctggtg gacattacca aagacgcgca gatggcatcc tgcgatttcg
     5341 agtgggactt cgtgatgcca cggttgcgca agcttgaaac ttgggaagca cccgatccgc
     5401 aacagctcgc cgaagccgcg gccctgatca acaaggctga gcgtccggtg attctggccg
     5461 gacacggcat cctgatgtct ggcgcgatgc aggacttgca gctgttcgcc gaagccagcg
     5521 atattcccgt cggcctgacg ctgttaggtc tgggcgcaat gccgcacttc catcctttgt
     5581 gcctgggcat gatgggcatg cacggcgaag catggttgaa tcacgccatc cagaacgccg
     5641 acttgctgat cgcgctgggc atgcgcttcg acgatcgtgt cacgggtaag ctcgacagct
     5701 acgcccagaa cgcgaagaag atccacgttg acatcgacgc ttccgagatt cataagaatg
     5761 ttcgcgctga tgtcgccatc gtgggcgatg taaaactggt gctgcctaag ctgactccgc
     5821 tgctcacgcc catgaagcgc tccacctatt gggcggagat caacaatgcc aagggcgcgg
     5881 tcgcagtgcg tgatattcgc aatctgcccg acaccggaca cctgtacgcc gcgcacgtga
     5941 ttcacgattt gtggcgcgag accaaaggca aggcgctgat cgtgaccgac gtcggtcaac
     6001 accagatgtg ggaggcgcag tattaccacc acagcgaacc tcgttcgctg atcacgtccg
     6061 gcggattggg cacgatgggc ttcgccttgc cggcggcgat cggagcgaag ttctcgcgtc
     6121 ccgatgcgga tgtctgggtc atcgtcggcg acggcggctt ccagatgacc atggcggagc
     6181 tggcgaccat ggtgcaggaa aacatcaagg tcaacatcgc catcatcaac aacggctacc
     6241 tggggatggt gcgccagtgg caggagttct tctacgagcg caactacgca gccacgccgc
     6301 tggtcaatcc cgatttcacg aaaatcgcgg aagcgtacgg catcctgacg ttcgcggtca
     6361 cgcatcgcga cgaggtcgct ccaacagtac aaaaggcgcg tgagcacccg ggcccggtgc
     6421 tgatcgattt ccgcgtggag caggaagatt cggtttatcc catggtcccg gcgggcaaag
     6481 cgctgcacga gatgatcagg cggcccagcg ccatcgtgga gacggctaca gacgagtaaa
     6541 tctatgaaca tgacctattt gttggacgtt gcgaatcata ccggcgtggt gacgcgcatt
     6601 tcgctgattt tccggcggca cggggccaac ctgcagtcgc tgtcgaccgc gaagaccgag
     6661 catccgtcga tcgttcgcat cgtgatcacc tcagacctcg agggccgagc ggcccaccgc
     6721 atcaaggggc agctctcgaa actcatcgac attttcaaag tggacatgat ctccaatgaa
     6781 gagggcacct tccgcgcctt cggcatgatg aagatcgcgt gcaccttgca gacacggcca
     6841 cagattttgc aattgctgga gatcttcaat gggcgcgtgc tcgaactcac cacggaatgg
     6901 atattggtcg agatgacggg cactccggaa cagctcgaag gcttgctgca ggtactgcaa
     6961 ccttacggaa ttctggaggc agtggagacc ggcccgatcg tcattcgccg caactcgccg
     7021 cgaaacggaa cacggccgca gccgacctgg gaagtagttc caggagcgtt gtctgcagcc
     7081 acaaattcaa ctactacttc aacttctaat tcaacgcagg agaggaaacg caatggcaca
     7141 gatcttttat gacaaggacg ccgatctttc gctggtgaaa gctacgaagg tcgccattat
     7201 tggttacgga tcgcaaggcc atgcgcatgc gctgaacctg cgtgacagcg gagtcgatgt
     7261 ttgcatcggg ctgcatccgg gcagccgctc acgagcgcgc gccgaggccg ccgggctgac
     7321 ggttaagtcg gtgagcgatg ccgcagcatg ggccgacacc atcatggtgc tggcgcccga
     7381 taccgagcag gcgaagattt atcaccgcga catccagccc aacctgaagc cgggcaagac
     7441 gctgatgttc gcccacggtt tcaacattcg cttcggaacc atcaagtgcc ccgctggggt
     7501 tgacgtcagc atgatcgcgc ccaaggcccc cggacatcga gtgcgcgagg tgtttgtcga
     7561 aggcggcggt acaccgtgcc tcgtggcggt ggagcaggat gcgtcaggca gggcatggca
     7621 gtacgcgctg tcatacgcga agggaattgg cggaactcgc gccggagttt tgaagaccac
     7681 gttcaccgaa gaaaccgaga ccgatctttt cggggaacag gccgtgttgt gtggcggcgc
     7741 cagtgccctg gtcaaagctg gcttcgagac gctcgtggac gcgggctatc aacccgaggt
     7801 cgcctacttc gagtgtctgc acgagttgaa gctgatcgtg gacctgatgt accgcggcgg
     7861 tctgagctat atgcgctact cggtcagcga tacggcagag cacggcgatt acaccgccgg
     7921 gaagcgcctg atcaacgacg aaacgcgcaa agagatgaag cagattctcg aagaaatccg
     7981 caacggcaag tttgcggctg cgtggatcaa ggagaacgag accgggcggc acaacttcat
     8041 cgcggaacgt gagcggcagc gcgaccacca gattgagagc gtgggagaac ggttgcgcga
     8101 catgatgccg ttcctcaatc ccgtgaaggt gtcggacatc gaagccccag caaagaagga
     8161 agaaccggtc gcggcagcca ggtagcgccg gccttcagag gctgccctga gcgtagtcga
     8221 agggccggcg ttaaggcggc ctattcagat tcctcgcgga ctttagtccg cgagggcttc
     8281 aaaccagggg atttcaggat ggcgtttttt tctgagagca agtgggtgtg gaagaacggg
     8341 aagatcattc cctgggaaca agccacggtc cacatgtcgg cgcacgggct gcactacggt
     8401 accggagtct tcgaaggcgt tcgctgctac aacacgccag atgggccagc ggtgttccgg
     8461 ctcgaggcac acttgcagcg catgttcgag tcggcccgaa cctatggcct tcccattcct
     8521 tactccgtgg aagaactgac gcaaggcgcg atcgacgtca tcaaagctaa cggcttctcc
     8581 gattgctacg tgcgtcccat agcgacgtat ggcagcgcga gtctcggtgt tcaccctcgg
     8641 aattgccccg tagaagtagc catgctggcg tggccatggg gcgcctacct cggcgcagac
     8701 gcgctggaaa acggtattca ggtaacggtt tcacgatgga agaagttcag ctcgctcgcg
     8761 atgccggcaa cggcgaaagc ctgcggtcaa tacctgaact cgatgttggc ggtgcaggat
     8821 gcagtgcgct gcgggttcga tgaagcactg ctggtggacg aagaaggagt catcgccgaa
     8881 ggctcgggcg agaacttctt catggtgcag aacggcaacc tcctcaccaa cgatgagcgc
     8941 gaccacgtgc tcatgggtat cactcgcgat tcggtgatca cactggcacg cgacctggga
     9001 tacaaagctt tcgtcggcca gatgcggctg gaagaactgg aagtcgccga cgaggcattc
     9061 ttcaccggca ccgcagcgga agtcacgccc atcagcgcgc tggatcgcaa gccgattggg
     9121 aatggaaagc ccggtcccgt cacgctggaa ttgcagcgcg cctacttcgc ggccacatca
     9181 ggcaaagacc caaagcatcg ggactggctg actcaggtga atgcgaagag cgaagtcaag
     9241 gagtcggtgt agcgttcagt gctcggagag catcagatac cgcgatccgg gagcttgcga
     9301 tacggcctta caagttttga ggaatgcttg tccagcatga gagagtacgg tgccagtcct
     9361 gtaaaccagg cgcagtttcc gctccatggc gagttcggga acgggtacgt gaatcaaatc
     9421 tccgcgagcg atctcatgtt ccacagcgat caacggaacc agggcaacac cgttctccat
     9481 gcgaacgaat tgtttgatgt cggcaatgtt gggcagctcg acattcatgt gcagcggagt
     9541 gtgatgtttt ttgaaggcct ggatcacctt ctcgcggtac ggactgagga cgctgtgggc
     9601 gatgaaggac tccgccccga gatctttgat gcccacatgc ttggcgtgcg ccagcggatg
     9661 gcgaggatag acgatcagag ccagttcgtc gcgatagagc acgtgggaag tgagttcgcc
     9721 gtcttcgggt cgaaaggaga cgacaccgaa ctccacttgg tggctgagca cctggccggg
     9781 gacttcgcgg gccagagtgc gcatgacctg gattcggatc atgggatgcg tgcggtggaa
     9841 ctggtgcagt actggaagca ggtagcgcga ggtgaactcg ttggcagcga tgaccagctt
     9901 gccctggtgg agcatgcgta gctccttgag cgactcgcgg gcctcttcgc gaacgttgag
     9961 cagctctttg gcatacgttt gcaggacctg gccggcgtcg gtcagcgaac cgtcacggga
    10021 agaacggtcg aagaggcgct ctccgagttc atcctcgagt ttggaaatgg tctggctgac
    10081 ggccgactgg gtacggtgca tgcgggctgc cgcccgcgaa aagctttttt cctcggctac
    10141 ggccaggaat gtctccagtt ggaagagctc catcattacc gcctttccat atttgggacg
    10201 gaataagcga ttgaatcatg tttgctaatg attctgcaag atatattatt ttgactaatt
    10261 gatgattagt cggtaacgtt aaaagatcac accttggagg tgaatatgca gcgagaccgc
    10321 gtattagttt ttgatacgtc gctccgcgac ggcgagcagg cccccggatg cagcatgaac
    10381 ggggacgaga agctcctatt cgccaaaaag ctccaagtgt tgggcgtcga tatcatcgag
    10441 gctggcttcc cggtcgcctc ggctggcgat accgaggcgg tacgtcgcat cgcaaccgaa
    10501 gttcagggcc cggtcatcgc ggcgctcgcg cgttgcgtcg aaaaggacat tgccgttgca
    10561 tgggaagcag ttagccgctc catgcggccg cgcatccaca cctttctggc cacctctgac
    10621 ctccacctcg cgttcaagct gaagatcggg cgcgacgaag ctctgaacca ggcccgcaag
    10681 atggtggcct atgctcgctc gctgtgcacc gatgtagaat tctcacccga ggacgcaacc
    10741 cggtcggacg taggtttcct gtgcgaggtg ttgcaggcgg cggtcgattc cggcgcttcc
    10801 acgctgaaca ttcccgacac ggttggctat accacgccgg gcgagtacgg agaactgatc
    10861 cgcaccattc gtcagcgggt caagggcatc gagaacgtta ctatctcggt tcactgccac
    10921 aacgatctgg gtctcgcagt ggcgaactcg ctggccggcg tcgaagcagg cgctcgccag
    10981 gttgagtgca cggtcaacgg catcggcgag cgtgccggca atgcctcgtt ggaagaattc
    11041 gccatggcgt tgcacgtacg cgcggatcgt tacggaatcg atacgggcat caaccacgcc
    11101 cagatttatt ccacgagcca gttgctctca gacttaattg ggttcgaagt gcagcctaac
    11161 aaggccatcg tcggacggaa cgctttcgcg cacgaggcgg gcatccatca gcacggtgtg
    11221 ctgaacaacc cgttgtgcta cgagatcatg acgccggaga cggtggggat tccggcagag
    11281 cgcatcgttc tcggcaagca ctcgggccgc catgcgctgg tctttaaata ccgtgaactc
    11341 ggtcacgaat tgtcgggcga cgaactcaac cggatctacc aggaattcac ccgcctggct
    11401 gatcgcaaga aggtcatcta cgaacaggac ttgctggcca tgctgtgcac ccatcgcagc
    11461 ggcatgcggg agcacactag tccctcaccg gagatgcggc cggcatgaag ttgcgcattg
    11521 tcgtattgcc gggagatgga atcggccctg aagtaaccag gcaggccgtg gctgtgctgc
    11581 agacggtttc ggatgtgtgc ggctacgagt ttcgtttcga gtcgcatcgc atcggcggag
    11641 cggccatcga gcaggatgga aagcctctgc cggacaaaac gctggcagcc tgcctggaat
    11701 ccgacgctgt gcttctcggt gcggtgggag cgcggaaatt cgatgaactc accggtgaga
    11761 agcgtcccga ggctggtctg ctcgcgctgc gacaggcgct cggcggattc gccaacctgc
    11821 gtccggtgaa agcatacgag gctatcgcgg attgttctcc tttgcgtgag gacgttacgc
    11881 gaggtgctga cattctgatt gtccgcgaat tgctgggcgg attgtatttc gcgcagccgc
    11941 gcgccctcaa tgaagacggc ctctcggcct tcaacaccat gcgctaccac gtggacgaag
    12001 ttgagcgtgt ggcacatgtg gcctttcagg aagcacgtcg ccgcaagcat ctcgttacgt
    12061 cggtggataa ggccaacgtg ctggagactt cacaactgtg gcgccgcact gtgaccgcac
    12121 tcgcaaaaga atatcccgac gtgcaactcg agcacgccta cgtcgattcc tttgccatgc
    12181 aggtgatcac gacgcccgcg cgctatgacg tggtgctgac cgaaaacttg ttcggcgaca
    12241 ttctgtcgga cgaagcggca gttatcagcg gatcgcttgg catgctggct tccgccagtg
    12301 ttggcggcaa ggtggaactc tatgagccgg tacatggttc cgctcctgat attgcgggca
    12361 aggaccttgc gaatccgttc ggcgcgatcc aatcggcagc catgttgctg cgctacaccg
    12421 ccgggctcta tcatgagtcg gacaaaatca tgcaggcagt cgaccgcact ctggcgcgcg
    12481 gactggtcac gcaagacctg agcggacggc gcaatcgccc ggctgtcccc actgacgcga
    12541 ttggccaggc agtggtcgaa accgtgactg acctgctgca aatgcagatg gcctaccacg
    12601 cagtctgagg caaagatgta caagccgaaa tctctatttg aaaaagtgtg ggactcgcat
    12661 gtagtgaggg cgcccgaagg cgagccgacg ctgctctaca tcgaccttca cctcgtacat
    12721 gaagtcacca cgccacaggc ctttgagggg ctgcgtctgg caaaccgcaa agtgcgccgg
    12781 ccagacctga cgtttgcgac catcgatcac aacgtcccaa cgacctccga cgagcgcttc
    12841 catatcaaag atcaagttgc cgcccagcag atcgccacgt tgcggcaaaa ctgcgaagcc
    12901 gctggcattc cgctattcga tattggatcg ggacggcagg gcatcgtgca cgtgattggt
    12961 ccggagctcg gcttgacgca gcccggcatg accatcgtat gcggtgacag ccacaccagt
    13021 acgcacgggg cgttcggagc tctcgcgttt ggcatcggca cttcggaagt cgaacacgtg
    13081 ctggccacac agtgcgtctc gcagaccaag ccgcgcagca tgcgtatctc ggtctcgaga
    13141 aggctgcccg aaggcacgac ttccaaggac ctgattctcg acatcatccg ccgccttggc
    13201 accgatggcg gtacgggaca cgtgatcgaa tatgcaggcg aggctgtccg cggactcacc
    13261 atggaagagc gcatgaccat gtgcaacatg agcatcgagt gtggagcgcg cgccggcatg
    13321 gtcgcgccgg atgaaatcac gttcgcgtat atggaggggc gcccatacgc accgaaggga
    13381 agcgcgtgga acaaggctgt caccgcatgg cggcagcttc cgagcgacga aggcgcgacc
    13441 ttcgaccgcg aaatctcgat tgatggctca aagctggagc cgatcgtaac ctggggaacg
    13501 aatcccggaa tgtcggtacc agtgactgcc aatgttccaa atcctgaaga tgcgcgcgat
    13561 gcttccgagc gcgccgccat gattgcggcc ctcgaataca tgggattgca agcgggaact
    13621 cgcgtcgaag acatcgcggt ggatcgtatc ttcatcggct cctgcaccaa cggacgtctg
    13681 gaagatttac gggctgcggc gaaagtcatt cacggacaca aagtgcatcc ggatgtgacg
    13741 gtgctggtag ttccaggctc cgaagcggtg aagttggccg ccgaacacga aggactcgac
    13801 cgaatcttca aagaagcggg cgcgcagtgg cgcgagccgg gatgctcgat gtgtctggga
    13861 atgaatccag ata
//
LOCUS       DASRKK010000237         6090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1092579, whole genome shotgun sequence.
ACCESSION   DASRKK010000237 DASRKK010000000
VERSION     DASRKK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092579"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VFC15_05190"
     CDS             <1..1458
                     /locus_tag="VFC15_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM09587.1"
                     /translation="AMSVDPSVHNIAFVGAANQLGMTGNQRSLVASIGTLSIAAAILA
                     TGSLGDHLGRKKIMQAGLLLAVIAGVVTSLAQNTAMFAAGRVIAGIGYAASFGLSFAL
                     LRAVAPDEAQMSRAVAQWLALQTVGVVVLGLLGGYLAGVGWRIAYLLPSTIAALAFLF
                     CLRNVPEARSDNLGPFDTFGLLLIAVGLVCTLDGISASASAGWGSPKVLIPLAIGILV
                     LLAFALYELRLQHPAFPIRLFRDPEMSAAALSGIAFNIGNAVVVLQLSLLWQYLYRYH
                     PFKVSLGLMPLSIASVVGATWVGSLLARGVSNRTLIPAGLFTVAASIAWMAFAWQSTP
                     YYFFLVPLSLAGVGMMFAQTPAARVFVSKSPPDLVGALGSSRTFFGQVGFALGLALSS
                     SFLYGLFSPELHSQLVRAGAAPGDLSQVSGIVQSYVSVGSAKAFNPQFAYEVLTKAIG
                     SYLFSYRIMMLALAALIALLGAVVCWQLPGRKSRA"
     gene            1489..3177
                     /locus_tag="VFC15_05195"
     CDS             1489..3177
                     /locus_tag="VFC15_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZM09588.1"
                     /translation="MMTNATIVPDVVYLGGDIITMDDENLVVEAVAVKDGKIAAVGTK
                     ADILTMSGAATRIVDLQGKTMLPGFIDGHSHFFQALMIADYVNVSAPPVGPGSSIADI
                     IATLKEHVAARLLQPGEWLIGYGYDGTALSDGREANVDDFDKDFTETPLVLVHVSGHG
                     CIMNSAGLRTVGIDANTPTPAGGVTVRKPGSNEPAGLLMENSWFPVLLALPKPSPQLL
                     LDNLDKAQQMYARTGYTTVQDAPVDPKVMPLYRKAADEGRFYLDLNGYWESHAFLANT
                     TLGNDFRSPRPDQYRLAGVKIIADGSPQAKTAYFTQPYLTGGPAGENNWRGTPTVTQE
                     HMNAVVKKAYEQDAQVLVHCNGDATIDMLLEAHKAAGAPRDRRTTVIHSQFVRRDQLN
                     DYVQLGVNPSFFTNHSFFWGDVHVQNLGRERAYFSSPMKTARSLGLRMTNHSDFLVTP
                     LDTMFILWTSVNRLSRSGQTIGPDECISVLDGLKALTIDAAYQYFEEDRKGSITAGKL
                     ADLVVLSDNPMKVDPVTIKDIRILETIKEGRTVFSRAPQVAIQPEGEPAGVGGR"
     gene            3325..4377
                     /locus_tag="VFC15_05200"
     CDS             3325..4377
                     /locus_tag="VFC15_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZM09589.1"
                     /translation="MSWRLLALCSVLFFGMPLCARDKTDVIVMTNGDRLTCAIKALNE
                     GTLFVSLDYVVGTISVQWSKVDHIESKQLFIVKTEDGFVYTGTLNSTNTAGRPLEIQV
                     LESGNKQVDISREQVVQMGQTSTSFWRRFNGAVNSGINYSKGNDAIQYNFSSQLEYLR
                     DRWSLEANYNSTLASSTGTDPSARNQLVLEGIHLLPWNNWFYSGLASFLQSSEQNIRL
                     QTTSGGGIGRYLANTNHASIAVIGGFAGQNTNYKQLAMPVNTENLAAAMLASEVKLFR
                     FNKTNLTVTSVLLPALSQPGRIEFTTNATYYIKIFSNLNWNISFYGNWDNQPPPSLQG
                     SDYGTSSGLSWTFGIR"
     gene            complement(4402..4629)
                     /locus_tag="VFC15_05205"
     CDS             complement(4402..4629)
                     /locus_tag="VFC15_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09590.1"
                     /translation="MLIPLLVVARSGDAQEDQNSTVQPYHILVGKAADTRPDLGLREQ
                     IKKTDDTHPASTVSVCALASHKRQPQKGEGD"
     gene            4998..>6090
                     /locus_tag="VFC15_05210"
     CDS             4998..>6090
                     /locus_tag="VFC15_05210"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM09591.1"
                     /translation="MFLRSNNRKKDGKDHRYYSIVENHRVASDKTVQRTVLYLGEIND
                     QQQAAWRKTLSVFDEEQQDYANLSLFPNDREIPADAVDSLQVKMSGLELRRPRLFGSC
                     WLACELWQQLGLREFWDARLTGSREEVPWEKVLRLLVVNRLLDPGSEFRVHRQWYLST
                     AMDALLGTDFAVAEKDRLYRCLDRVLEHKQELFLWLRQKWADLFQAEFEVLLYDLTST
                     YFEGAMEENPKAKYGHSRDKRTDCLQVVIALVITTDGFPLAYEVLDGNTSDRTTLRAF
                     LDHIEKTYGKAKRMWVMDRGIPTEEILREMRDPAREIFYLVGTPKSKIQQYEKKWLDL
                     PWQKVRESVEVKLFEQEGELYVLAKSDGRR"
BASE COUNT         1271 a         1696 c         1776 g         1347 t
ORIGIN      
        1 gcgatgtcgg tcgacccttc cgtgcacaac atcgccttcg tcggtgcagc caaccagcta
       61 ggcatgaccg gcaaccagcg ttcgttagta gccagcatcg gaacactctc tatcgccgcc
      121 gccatccttg ccaccggcag cctgggtgac cacctgggac gaaagaagat catgcaagct
      181 gggctgcttc ttgcggtgat cgccggggtt gtaacttcgc tggcacagaa caccgccatg
      241 ttcgccgccg gccgggtgat cgcgggaatc ggttatgcgg catccttcgg actttccttc
      301 gcgttgctgc gggcggtcgc gccagacgaa gcacagatgt cgcgcgccgt cgctcagtgg
      361 ctcgcgctgc agacggttgg agtggtggtg ctgggtctgc tcggcggtta ccttgcggga
      421 gtgggctggc gaatcgcgta ccttttgccg agcaccatcg cagccctggc atttctgttt
      481 tgtcttcgta atgtcccgga agcgcgctcc gacaacctgg ggcctttcga taccttcgga
      541 cttttgctga tcgcagttgg cttagtctgc acgctggatg gtatcagcgc ttcggcctct
      601 gccggatggg gcagccccaa ggtcctcatt ccgctcgcga tcgggatatt ggttctgctg
      661 gcgtttgcgc tgtacgaatt gcggctgcag catccggcgt ttcccatacg tctgtttcgt
      721 gacccggaga tgtctgctgc agccttgagc ggaatcgcgt tcaacattgg caacgccgtc
      781 gtggttttgc agttgtcatt gttgtggcag tacttgtatc gctatcaccc attcaaagta
      841 agtcttggcc tgatgccgct cagtatcgcc agcgtcgtgg gtgcgacgtg ggtcggctcg
      901 ctgctcgcgc gcggcgtctc caaccgcacg ttgataccgg ccggactctt caccgtggcg
      961 gcatccatcg cgtggatggc ctttgcgtgg cagtccacgc cgtattactt cttcctcgtc
     1021 ccgctatcgc tcgcgggggt gggaatgatg tttgcgcaga ctccggcggc gcgcgttttt
     1081 gtcagcaagt ctccacccga cctggtcggt gctctgggtt cgtcgcgaac atttttcggc
     1141 caggttggct ttgctctcgg actggcgctc agttcctcgt tcctttacgg actcttcagc
     1201 ccagagttgc attcgcaact ggtgcgcgca ggggccgctc ccggcgactt gtcgcaagtg
     1261 tcggggatcg ttcagtcata cgtcagcgtg ggaagcgcca aagcattcaa cccgcagttc
     1321 gcctacgagg tcctgacaaa agctatcggc tcctatttgt tcagctaccg aatcatgatg
     1381 ctggccttgg cagccctgat cgcacttctt ggcgcggtgg tttgctggca gcttccgggt
     1441 cgcaagtcca gggcataaga acgaactgcc atcagagagg aaatagacat gatgacaaac
     1501 gcgacgattg ttcccgatgt ggtctatctt ggcggcgaca tcatcaccat ggatgatgag
     1561 aatctcgtag tcgaggccgt ggccgtcaaa gacggcaaga tcgcggccgt gggtacgaag
     1621 gccgacatat taacgatgtc cggagcggcc acaagaatcg tcgatctgca aggcaagacc
     1681 atgctgcccg gatttatcga cggtcacagc cacttcttcc aggcactgat gattgccgat
     1741 tacgtgaatg tgtccgcgcc tccggtggga ccgggatcga gcatcgccga catcatcgcc
     1801 acgttgaagg aacacgttgc cgcccggctg ctgcaacccg gggaatggct gatcggatat
     1861 ggctacgatg gaacggcgct ctccgacggg cgcgaagcca acgtcgacga cttcgataag
     1921 gattttactg aaacgccgct ggtgctggtt cacgtttccg ggcacggctg catcatgaac
     1981 tcggctggat tgaggacggt cggcattgac gcgaacacgc ccactccggc aggcggcgtc
     2041 accgtgcgga agcccggcag caacgagccg gcagggctgc tgatggagaa ctcctggttc
     2101 ccggtgctgc tcgcgcttcc caagccgtct ccacagctgt tgctcgacaa tctcgacaaa
     2161 gcgcaacaga tgtatgcccg caccggctac accaccgtgc aggacgcgcc cgtcgatccc
     2221 aaggtaatgc cgctctatcg caaggcagcc gatgagggcc gcttctatct cgatctgaac
     2281 ggctactggg agtcacacgc attccttgcc aacacgacat tgggcaacga cttccgatcg
     2341 ccgcggccgg atcagtatcg gctcgccggc gtcaagatca ttgctgacgg atcgccgcaa
     2401 gccaagaccg cctacttcac ccagccgtat ctcactggcg gtcccgcagg cgagaataac
     2461 tggcgcggta cgccgacagt aacgcaagag catatgaatg cggtggtgaa gaaggcctac
     2521 gagcaggacg cccaagtgct ggtgcattgc aacggcgacg ccacgatcga tatgttgctt
     2581 gaggcgcaca aagcggcggg cgcgccgcgc gatcgccgta ccacggtcat tcactcacaa
     2641 ttcgtgcgac gcgatcagct taacgactat gtccaactgg gagtgaaccc atcgttcttc
     2701 accaatcact ctttcttctg gggcgatgtg catgtccaga acctcggtcg ggagcgggcg
     2761 tatttttcca gtccgatgaa gacggcgcgt tcgctcggct tgcgcatgac gaaccacagc
     2821 gacttccttg tgactccttt ggacaccatg ttcatcctgt ggacctcagt caatcggctc
     2881 tcgcgctccg ggcagacgat cggaccggac gaatgcatct cggtgcttga cgggttgaag
     2941 gcgcttacca tcgatgccgc ttaccagtac ttcgaggaag atcgcaaagg ttccatcacg
     3001 gccggcaaac ttgccgatct ggtggttctg tccgacaacc cgatgaaggt cgatccggta
     3061 accattaagg acattcgcat tttggagacg atcaaggaag gccgaacggt gttcagccgt
     3121 gccccgcagg ttgccatcca gccggaaggg gaacctgcag gtgtcggcgg gcgatgacaa
     3181 agcgatactg acgttacttg atcgtcagcg aaaaagaagc cggccggcac tcgttcgaga
     3241 catgtccacc tgattgccca ttcagtcacg ttcagccccc gatccccgtt cgatgtcaac
     3301 ttgcgaagta tctctggagg cattatgagc tggcgattgc ttgctctctg cagtgtgctt
     3361 ttctttggca tgcctttatg tgcccgcgat aagaccgatg tgatcgtgat gacaaatggc
     3421 gatcgtctga cctgcgcgat caaggctctc aatgaaggca ctctgttcgt gagcctggat
     3481 tacgttgtgg gcaccatctc agtgcagtgg tcgaaagtcg atcacatcga gagcaagcag
     3541 ctctttatcg tgaagaccga ggatgggttc gtgtacaccg gaacgctcaa cagcaccaac
     3601 acagcgggtc gtccgcttga aattcaagtt ctcgagagtg gaaacaagca agtagacatt
     3661 tcgcgtgaac aagtagtgca aatgggtcag acctccacca gcttctggcg ccgcttcaat
     3721 ggagcagtca attccggtat caactactcg aaaggcaatg acgccatcca atacaacttc
     3781 agctcacaac tggaatacct ccgggatcgc tggtcgctgg aggcaaatta caactcgaca
     3841 cttgcctcaa gcacggggac agatccctca gctcgaaatc aacttgtgct cgagggcatt
     3901 cacttgttgc catggaacaa ctggttctac tctggcctcg cgagctttct ccagagttct
     3961 gaacaaaata ttcgccttca aaccacgagc ggcggcggca ttggccggta cttggcgaat
     4021 acgaatcatg caagcattgc ggtcataggc gggttcgccg ggcagaacac gaactacaag
     4081 caattggcga tgccggtaaa caccgagaac cttgctgccg ccatgctagc gtcagaagtg
     4141 aagctgtttc gcttcaataa aaccaacctc accgtgacat cagtcctctt accagcgctg
     4201 tcgcagcccg ggcgaatcga gttcactacg aatgccacct actacatcaa gatttttagc
     4261 aatctgaact ggaacatctc gttctacggc aattgggaca accaacctcc tccaagccta
     4321 caaggcagcg attatggcac cagttccgga ctgagctgga catttggtat taggtaacgg
     4381 cagtacaagt gtgaccttgt ttcagtctcc ttcacccttc tgcggctgtc gtttgtggct
     4441 agccaaggcg caaacagaga cggtgcttgc gggatgggta tcgtcggttt tcttaatctg
     4501 ttcgcgaagg ccaagatccg ggcgcgtgtc ggccgccttg ccgacgagga tatggtacgg
     4561 ctgaaccgtg ctgttctggt cttcctgggc atcgccgctc cgagcgacaa cgagcaacgg
     4621 aatcagcata aggtttgttc atcttcgaga tcaccagccg atgtttggcg ctgtcgatcc
     4681 cttatcggca agcctgcctt ggtgttggga aggagtggat ccgccgactc ttggccgaca
     4741 tgaaggaacg aggtgaaagc gcggtccagg tgcgcgaacc cgccgaccgc tggcggtacg
     4801 tgcggatgag cactgagggt accacttcca cgtgagcgta ttagccccac cttcgcagtt
     4861 gccggcgttt ttcggctttt tgaggctaat ttgtatggtt aagccgtttg ttttcacggt
     4921 cactctctga aaaaacggta gagtggagac caagtacctt cccttcgaca ccggatttct
     4981 ggcactctgc agtcggcatg tttctgcgca gcaacaatcg caaaaaagac ggcaaggatc
     5041 atcgctatta cagtatcgtc gagaaccatc gcgtggcttc tgacaagacg gtgcagcgaa
     5101 ccgttctcta tctcggtgag atcaacgatc agcaacaagc ggcctggcgc aagacgctgt
     5161 cggtgtttga tgaagagcag caagattatg cgaacttgag tttgtttccc aacgatcgcg
     5221 agattcccgc cgatgcagtc gacagcctgc aagtgaaaat gagcggtctg gagctgcggc
     5281 gtcctcggct gttcggcagt tgctggttgg catgtgaatt atggcagcaa ctggggttgc
     5341 gtgagttctg ggatgctcgt ctcaccggat cgcgagaaga ggttccttgg gaaaaggtgc
     5401 tgcgcctcct ggtggtaaac cgactgctgg atccgggcag cgagtttcgg gtgcatcgcc
     5461 agtggtacct gagcaccgcc atggatgccc tgctgggaac ggatttcgcg gtggccgaga
     5521 aggatcgctt gtatcgctgt ttggatcggg tgctggaaca taagcaggaa ctctttctat
     5581 ggctacgcca gaagtgggcc gatctgtttc aagcggaatt tgaagtgctg ctctacgact
     5641 tgacgagcac ctactttgaa ggcgcgatgg aagagaatcc caaagccaag tacgggcaca
     5701 gtcgcgataa acggacggac tgtttgcagg tggtgattgc gctggtgatc accaccgatg
     5761 gttttccctt ggcctacgaa gtgctggacg gcaataccag tgatcgcacc acgctgcggg
     5821 cctttctcga ccacatcgag aagacctatg gcaaagccaa acgcatgtgg gtgatggatc
     5881 gcggcattcc cacggaagag atattgcgcg agatgcggga tccggcacgg gagattttct
     5941 atttggtcgg aaccccgaaa agcaagatcc aacagtatga aaagaagtgg ctcgatctgc
     6001 cctggcagaa ggtcagagag tcggtggaag tgaaactgtt cgagcaggaa ggcgagctgt
     6061 atgtgttggc caagagtgac ggaagaagag
//
LOCUS       DASRKK010000238         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_408545, whole genome shotgun sequence.
ACCESSION   DASRKK010000238 DASRKK010000000
VERSION     DASRKK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408545"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..606
                     /locus_tag="VFC15_05215"
     CDS             307..606
                     /locus_tag="VFC15_05215"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZM09592.1"
                     /translation="MRPASSTLKKIFADTLRREGGDSAPLLAWPLACGSKVAEKTSAI
                     GYADGVLTVEVPDATWRQQLEGLSRQYIVALKQISAQPVNAIKFVVRNQTERPSR"
     gene            632..961
                     /gene="gatC"
                     /locus_tag="VFC15_05220"
     CDS             632..961
                     /gene="gatC"
                     /locus_tag="VFC15_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZM09593.1"
                     /translation="MVTDKDVSYVAELAHLELTEHERARLLKDLNSVLGYIDRLNELD
                     TTNVEPMAQVASRPPRRGGETSGTEGFAYAMRADEPRPSLPHDEALRNAPASDGVFFK
                     VPKVIER"
     gene            998..2437
                     /gene="gatA"
                     /locus_tag="VFC15_05225"
     CDS             998..2437
                     /gene="gatA"
                     /locus_tag="VFC15_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZM09594.1"
                     /translation="MNIDLLTVDSARTAVLEKQTTASALVDAFYKKIDAEDAAIGAYL
                     TLCKERAYQQAARVDALADKGDALPPLAGVPIAVKDVFATKGVRTTAGSKILQNFVSP
                     YDATVVARLEEAGAIILGKTNCDEFAMGSSNENSGFHPVHNPRDHSRVPGGSSGGSAA
                     VVAAGTAVASIGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLIAFASSLDHVGPFGK
                     SVKDTALMLQYMAGREPLDSTSADVPVPDYLDQIEKPVRGLKIGIPEEYFGEGLDPEV
                     RSAVEAGIQELAKAGCEIVPISLSNTKYAIPTYYVVATAEASSNLARYDGVRYGVRAK
                     DGKTLAEMYRRTRDAGFGAEVKRRIMLGTYVLSAGYYDAYYLKAQRVRRLLASDFDQA
                     FTNVDAIVTPTTPTPAFKLGEKMDDPLAMYLADIFTVTANLAGIPGMSIPCGKSNEGL
                     PIGMQILGKHFDEPMVLRVGHAAEHALAG"
     gene            complement(2690..2920)
                     /locus_tag="VFC15_05230"
     CDS             complement(2690..2920)
                     /locus_tag="VFC15_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZM09595.1"
                     /translation="MPAVATKRRVKLFTIELDREEDGRWIADVPDLPGVIVYGDAKAD
                     ALAKVQALALRVLTSKIEEDGTPQDEVRFVLA"
     gene            3083..3367
                     /locus_tag="VFC15_05235"
     CDS             3083..3367
                     /locus_tag="VFC15_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09596.1"
                     /translation="MADHVIHISEAEAASDFASVLARVRAGAEVVIESEGGKLPVAVI
                     HPPVPARRTISECIALLPEDSTAVMDADFAKDVEAAIESHREPLEPLPWD"
     gene            3358..3489
                     /locus_tag="VFC15_05240"
     CDS             3358..3489
                     /locus_tag="VFC15_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09597.1"
                     /translation="MGLILDSSVLITAERQGQNARQMLAAISGSPAGNTETQSPWSR"
     gene            3477..3773
                     /locus_tag="VFC15_05245"
     CDS             3477..3773
                     /locus_tag="VFC15_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM09598.1"
                     /translation="MVTLIELAHGAARADTPQRRAKRHQFIQELLTALPIHPITVQVA
                     LRTGQIDGENQAKGVRLPLSDLLIGVTALELGYSVATANLRHFQMIPGLKIVSL"
     gene            complement(3870..4454)
                     /locus_tag="VFC15_05250"
     CDS             complement(3870..4454)
                     /locus_tag="VFC15_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZM09599.1"
                     /translation="MKTFDELLRDAEVAHGHLCAGQVLGVRLAMLGLELLGIDDPRGK
                     DRKRLITFVEIDRCATDAVAVVTGCRLGKRALKFRDWGKVAATFVDLASQRAVRVCAR
                     ESSKTLARQMHPEVENKNQQQMLAYREISNDDLFTVQWVKVSLPPEELPGYKGERIVC
                     ERCGEGINFQREVRRDGKIVCRACGGEKYYEEIS"
     gene            complement(4474..>5175)
                     /locus_tag="VFC15_05255"
     CDS             complement(4474..>5175)
                     /locus_tag="VFC15_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09600.1"
                     /translation="QRRRTGNSLPLAMRTVHVLVVLMSLNIAISAASKPHVVSFGKPQ
                     PVKVFIGAAEIRTMDITVSPLFVDAKLKEYTTGKQHDVTDRQFVVRRAFRINDALPDE
                     ARKSPKWLWELGGWLLVDRSSGRITPIKLLDFDPFYSEVSWYRDYAAYCGTTANGEKL
                     DAVVVQIGVKKPLYRKELGKANGGEQSDSDCSAPQWNRQPARVTFLPKVGDKLSVTVR
                     GRFVDEVPDDDSDEP"
BASE COUNT         1057 a         1571 c         1518 g         1029 t
ORIGIN      
        1 tgaacggaga agacctgatc gccctcggct accggcccgg gccgcagttt agctccatgc
       61 tcgcggcgat tgaggacgcg cagttggaag ggacgctcaa gggtagagaa gacgcgctgg
      121 agtttgtgcg tcgggagttt cctcatgcgg gctaagaaag caggttcctc cgctcgcccg
      181 tcgcggcggg ctcggtctgg atgacagcga ggaggggata atccttcggt cggcaggaca
      241 gcgattcacg cgacgagcga agagcgaaga gcctgccagt tgctctacaa tcgaagctcc
      301 gacgttatgc gacctgccag ctcgacatta aagaagatct ttgccgacac tttgcgtcga
      361 gagggcggag acagcgcgcc gctgctggca tggccgctgg cctgcggatc gaaggtcgct
      421 gagaaaacaa gcgccatcgg ctatgcggat ggagtgctca cggtcgaagt tcccgatgca
      481 acctggcgac agcaactgga gggcctcagc cggcaatata ttgtggccct gaagcagatt
      541 agcgcgcagc cggtgaacgc gatcaagttt gtagtgagga accaaaccga acggccgtcg
      601 cgctagaaag cagatcgtta gaaggcacat catggtcacc gataaagacg tctcctacgt
      661 tgccgagctc gcgcatctcg aactgaccga gcatgagcgc gcccgcctgc tgaaggacct
      721 gaactccgtc ctcggctaca ttgatcgcct gaacgagttg gacacgacca acgtcgagcc
      781 catggcgcaa gtcgccagcc gcccgccgcg gcggggcggc gagaccagcg gtaccgaggg
      841 attcgcttat gccatgcggg cggacgaacc gcgtccttcg ctgccccacg atgaagcgtt
      901 gcgcaatgcg cccgcttcgg atggcgtctt cttcaaagtg cccaaagtga ttgagcgtta
      961 ggcgaagctc acagaacccg cagatactcg gacccacatg aacatcgatt tattgacagt
     1021 tgattcggcg cgaacggcgg tgctggaaaa gcagaccacg gccagtgcgc tggtggacgc
     1081 cttttacaag aagatcgatg ccgaagatgc cgccatcggc gcttatctca cattgtgcaa
     1141 ggagcgggcc tatcagcagg cggcacgggt tgacgcgctg gccgacaaag gcgatgcgct
     1201 gccgcccctg gctggggttc cgatcgcggt gaaggatgtc ttcgcgacga aaggcgtacg
     1261 aaccaccgca ggctcgaaga tcctgcagaa cttcgtctct ccgtacgacg ccacggtggt
     1321 ggcgcggctg gaagaagctg gcgccatcat tctgggcaag accaattgcg acgagttcgc
     1381 catgggctcg tcgaacgaaa attccggctt tcacccggta cacaatccac gtgatcactc
     1441 gcgagtgccg ggcggatcct cgggcggctc cgccgcagta gtcgctgcgg gaaccgccgt
     1501 ggcttcgata ggttcggata cgggcggctc gattcggcag ccggccgcat tctgcggcgt
     1561 cgtcggtctg aagcccacct atggacgggt gtctcgctac gggcttatcg ccttcgcgtc
     1621 gtcactcgat cacgttggcc cttttggcaa gagcgtgaaa gatacggctt tgatgctgca
     1681 gtacatggcc ggccgcgagc cgctcgattc gacatcggcc gacgtacctg tacccgacta
     1741 tctcgatcag attgaaaagc cggtgcgagg tttgaagatc ggaattccgg aagaatattt
     1801 cggcgaggga ctcgatccgg aagttcgcag cgcggtggaa gcgggcatcc aggagttagc
     1861 caaagccggt tgcgagatcg ttccgatatc gctgtccaac acgaaatacg ccatcccaac
     1921 gtactacgtt gtcgccaccg ccgaagcctc gtcgaacctg gcgcgctatg acggcgtgcg
     1981 ttacggggtc cgcgcgaaag atgggaagac cctggctgaa atgtaccggc gcacgcggga
     2041 cgcgggtttc ggagcggaag tcaaacggcg catcatgctt ggcacatacg ttctgagtgc
     2101 tggatactac gatgcctatt acctgaaggc gcaacgtgtg cgccgtttgc tggcgagcga
     2161 cttcgaccag gcattcacca acgtagatgc gattgtcaca cccaccacgc cgacgcccgc
     2221 attcaaactc ggcgagaaga tggacgatcc actggccatg tatctggcgg acatttttac
     2281 ggttacggcg aacctggccg gcattccggg gatgtctatt ccgtgcggca agagcaacga
     2341 gggtttgccg atcggaatgc agatactggg aaagcacttc gacgagccca tggtgttgcg
     2401 ggtggggcac gcggcggaac acgcgctggc ggggtagagc agcccagcga tcgccgtgtt
     2461 caactgctac agcagattag aggtcttcgc gctttaaccc tgtctttttt ggagattttg
     2521 gcgagcatcg cggggcccag ttcgtctgaa tcgtcgaagg cccacgtgta ttcgcctagc
     2581 ttggggtgaa ccaattgcac atgggaagac gctttgtatc ccttgatcgt ccaaccaatt
     2641 cgaccaaggg ctttcaggac tttcccagcc ttggtgcttg gccagcgact cacgctaaga
     2701 cgaaccgcac ttcatcctgt ggcgttccat cctcttcaat cttgctagtc aacaccctta
     2761 acgccaacgc ctgcaccttg gcaagtgcat ctgccttcgc gtctccatag acgatgacgc
     2821 ccggaagatc gggaacatcg gctatccagc gtccatcttc ctcccggtcg agttcgatgg
     2881 taaacaactt aacgcgtcgc ttggtcgcaa cggctggcat taaattcctc tctgtgaaaa
     2941 ctgatttctt cgacactatg agtgtacccg aactgtcaat caggtgggcc gtacattctg
     3001 ttggagcgga acaccttgat ttcagctggt tgatgttgat tttccaggct gggagggttg
     3061 cataaaccgg tataatcgcg ccatggccga ccatgtcatc cacatctcgg aagcggaagc
     3121 ggccagcgac ttcgcgtcgg tgctggcccg cgtgcgtgcg ggggcggaag tcgtcatcga
     3181 gagcgagggg ggcaagctgc ccgtagcggt gatccatccc ccagtaccgg cccgccgcac
     3241 catctccgag tgcatcgcgc tgttgcccga agactcgaca gcggtcatgg atgcagactt
     3301 cgccaaggat gtcgaggcag ctatcgagag ccaccgcgaa ccgctagaac cactgccatg
     3361 ggactgatcc tcgactccag cgtactgatt acagccgagc gccaaggaca gaatgcgcga
     3421 cagatgctcg cggccatctc cggcagccca gcgggcaata ccgagacgca atctccgtgg
     3481 tcacgctaat tgaattggcg cacggtgcgg cccgcgctga cacaccgcag cgaagagcta
     3541 agcggcacca gtttatccag gaactgctca cggccctgcc catccatccc atcaccgtgc
     3601 aggttgccct acgcaccggc cagatcgacg gcgagaacca ggcgaagggc gtccgccttc
     3661 ctctctccga tctgctgatc ggggtgaccg ccttggaact cggctacagc gtcgcgactg
     3721 ccaaccttcg ccacttccag atgatccccg gcctcaagat cgtctccctg taggccgcgt
     3781 tctcgtggcg acggaccccg ttggctgccg gcaatcccaa gcttgttgcg gcgccaagcc
     3841 gccacgccgc gatcccgcgg catcttcccc tacgaaatct cttcgtaata tttctctccg
     3901 ccgcaagcgc ggcacacaat ttttccgtca cgccgtactt cgcgctggaa gttgatgcct
     3961 tcgccacatc gctcgcacac gatccgctcg cccttgtagc cgggtaattc ttccggcggt
     4021 agagaaacct ttacccactg cacggtgaac aggtcgtcgt tcgatatctc gcgataagcc
     4081 agcatctgtt gctggttctt gttctcgact tcgggatgca tttgccgggc caacgttttc
     4141 gagctttccc gggcgcaaac ccgcaccgct cgctgcgagg cgagatcgac aaacgtcgcc
     4201 gccactttac cccagtcacg gaacttgagc gcccgcttgc ccagccggca tccggttacg
     4261 acggcaacgg cgtcggtggc gcagcggtca atctccacaa acgtgatgag acgtttgcga
     4321 tccttcccgc gcggatcgtc gatgcccagc agctccagcc caagcatggc gagacggacg
     4381 cccagcacct gccccgcaca caggtgtccg tgggctacct cggcgtcgcg cagcagttcg
     4441 tcaaaagtct tcaatggaaa ctcgcactcc gtcttatggc tcgtctgaat cgtcgtcggg
     4501 cacttcgtcc acaaagcggc ccctgaccgt cacgctgagc ttgtcaccaa ctttgggaag
     4561 aaaggtcacg cgcgcaggtt gacggttcca ttgcggggca gaacaatcgg agtcggattg
     4621 ttcaccgccg tttgccttgc ccagctcttt gcgatacagc ggcttcttga cgccaatttg
     4681 cacgacaacg gcatcgagct tttcaccatt ggccgtcgtt ccgcagtacg ccgcatagtc
     4741 acggtaccaa cttacctccg agtagaacgg atcgaaatcg agcaacttga ttggcgtgat
     4801 tcttccggaa ctacggtcta ccagcagcca cccaccaagt tcccagagcc acttgggaga
     4861 cttgcgtgcc tcgtcgggca gcgcgtcatt gatgcgaaaa gcccggcgta ccacgaactg
     4921 ccgatcggtg acgtcatgtt gcttacccgt cgtgtactct ttcagcttcg catccacaaa
     4981 caggggactg accgtaatgt ccatcgtcct gatttccgca gcgccgatga agaccttgac
     5041 cggctgcggc tttccgaagc ttacaacgtg gggtttggaa gcagcgctga tcgctatatt
     5101 caacgacatc aggacgacga gaacgtgaac ggtgcgcatg gctaagggaa ggctattccc
     5161 ggttcgtcgc cgctg
//
LOCUS       DASRKK010000239         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_896842, whole genome shotgun sequence.
ACCESSION   DASRKK010000239 DASRKK010000000
VERSION     DASRKK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896842"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2445
                     /locus_tag="VFC15_05260"
     CDS             <1..>2445
                     /locus_tag="VFC15_05260"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM09601.1"
                     /translation="IGTILKDYGSRDRRIKVTFRATNGHIAGASNSALEMVTGDFVAL
                     MDHDDELTPHALYMVAVELNENPDTDIIYSDEDKIAADGRRHGPFFKTDWNRELFYAQ
                     NMVNHLGVYRASLVKEMGGFREGFEGSQDYDLALRILARTEAERIRHIPYVLYHRRGA
                     KDGETFSIVNRPTAAEAARRALADHFESKGEAVRVVSDATGWFNRVICPLPDPAPRVS
                     LIVPTRDKVALLRTCIDGLLHQTRYPNLEVIIVDNDSCEPETLEYFESLRNDSRVRIL
                     HIGGAFNYAAFNNQAVASAGGDLIGFVNNDIEVIEPGWLEEMVSQATQPGVGAVGAKL
                     YYRDDTIQHAGVVLGMRDVADHVHKHLPRSASGYFGRLQVVQNVSCVTAACMILPKRV
                     FVEVGGFDEINLKVAYNDVDLCIRIREAGYSIIWTPYAELYHLESASRGYGCAPENFD
                     RETREAFYMKRRWGTLLAADSFHSPNFTLKDPSLDLAFPPRVGKPWRRWKTIAGEQEP
                     FRVQQDNEGPVPELSEVFSYISTSVDASALPKRVAIGVSSLGNFFMAEIARMLEDAFR
                     QLGVPARVFTESEALTVSSDDTVLVVAPHEFFLLGEGPRAAAMLKYVPTLVMVNTEQR
                     QTSWFALAEPYLRQASAVLDINYESARQLVRSGYKAFALPLGYSDYIAQHFDGRVLPE
                     HELLKHMPSSVFGKIPSSYSDRPIDVLFVGTSSPRRQKFFSQNAGFFAGKNTFIYMPD
                     GDKPFSANESRTIDFSTFAALVRRSKVLLNIHRDDTPYLEWQRIVTLGIMQKTLVVTE
                     HCQPGPCIEPNVDYLDG"
BASE COUNT          533 a          666 c          673 g          573 t
ORIGIN      
        1 gatcgggacc attctgaaag actatgggtc acgcgatcgc cgcatcaagg tcaccttcag
       61 ggctacgaac ggtcacatcg ccggcgccag caattcagca ctcgaaatgg tcactggcga
      121 ctttgtcgca ctgatggacc acgatgacga gctaactcct catgccttat atatggtggc
      181 cgtcgaacta aatgaaaatc ccgatacgga catcatttat tctgatgagg ataagatcgc
      241 tgccgacgga cggcgccacg gaccgttctt caagaccgat tggaaccggg agctcttcta
      301 cgctcagaac atggtcaacc atctcggcgt ctaccgggct tcgttggtga aggagatggg
      361 tggtttccgc gaaggattcg aaggaagcca ggattatgat cttgcgcttc ggatattggc
      421 tcggaccgaa gcggagcgaa ttcgccatat tccgtacgtc ctctaccatc ggcggggggc
      481 gaaagacggg gaaacgttct ccatcgtcaa tcggccaacc gcagccgagg ccgcccgtcg
      541 tgcgctcgct gaccactttg agtctaaggg tgaagcggtg cgggtggtca gcgacgctac
      601 tggatggttc aatcgagtca tttgtccgct gcccgatcct gcgccgcgcg tctccctcat
      661 cgtaccgacc cgcgacaagg tagcgctgct gcgcacctgc attgatggac tactgcacca
      721 gactcgctat ccaaacctcg aggtcattat tgttgacaat gatagctgcg aacccgaaac
      781 cctggagtat ttcgaaagct tacggaatga cagccgggtg cgcatcttgc atatcggagg
      841 ggccttcaac tatgcggctt tcaataacca agccgtcgca agcgcggggg gtgacctgat
      901 cgggttcgtc aataatgata tcgaggtcat cgaacctggt tggctcgagg agatggtaag
      961 ccaggcgacg cagccgggcg tcggggctgt cggcgccaaa ctctactatc gcgatgacac
     1021 gattcagcac gcgggcgttg tgcttggtat gcgtgacgtg gcggaccacg ttcacaagca
     1081 tcttccacgt tcggcatccg gttactttgg ccgtctgcag gtggtgcaaa atgtctcctg
     1141 cgtaaccgcg gcctgcatga ttcttccaaa gcgcgtcttt gttgaagtcg gcggcttcga
     1201 tgaaatcaat ctgaaggtcg cctacaacga cgtcgatctc tgcataagaa ttcgggaggc
     1261 gggctacagc attatatgga ctccgtatgc agaactgtac cacttggagt cggcctcacg
     1321 cggctacggg tgcgcaccag aaaatttcga ccgggagacc cgcgaggcgt tctacatgaa
     1381 gcggcgttgg gggacattac ttgcagcgga ttcgttccat agtcctaact tcacgttgaa
     1441 agaccccagc ttagacttag cctttccgcc gcgcgtcggc aagccctggc gtcggtggaa
     1501 aaccatcgcg ggtgaacagg aaccctttag agtccagcaa gataacgagg gtccagttcc
     1561 tgagctatcg gaagttttct cctatatatc tacttcggtt gatgcttcgg cattaccaaa
     1621 gagggtcgcg attggcgtgt cgagtctcgg caacttcttt atggccgaga ttgcgcgcat
     1681 gttggaagat gctttccggc aactcggtgt gccagcaaga gtgtttaccg agtccgaagc
     1741 tcttacggtc tccagcgatg acacggtgct cgtagtcgcg ccacatgaat tcttccttct
     1801 aggtgagggc cctcgcgcag ccgctatgct aaaatacgtg cccactcttg tgatggtgaa
     1861 tacagaacag cgtcaaactt catggttcgc tctggctgaa ccatacttaa gacaagcttc
     1921 agcggtactc gatattaact atgagtcggc ccggcagctt gtacgctcag gatacaaagc
     1981 gttcgcgttg ccgctgggat atagcgacta tattgctcaa cattttgatg gacgcgtact
     2041 cccggaacat gaattgctca agcacatgcc ctcctcggtc tttggtaaga taccttcgtc
     2101 ttattctgat cgaccgatcg atgtgctttt tgtgggcact tcatcgccac gtcggcagaa
     2161 gtttttctcc cagaacgcag gcttttttgc tggcaagaat actttcatct acatgcctga
     2221 cggcgacaaa ccgttctcgg cgaacgaatc caggaccatc gatttctcga cttttgctgc
     2281 tttggtccgg cgctccaagg ttctgctcaa tattcaccgt gacgacaccc cttacctgga
     2341 atggcagaga attgtgaccc tgggcatcat gcagaagacc ctcgtggtga cggagcactg
     2401 ccagcctggg ccctgcattg aacccaacgt ggattacctg gatgg
//
LOCUS       DASRKK010000240         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_310652, whole genome shotgun sequence.
ACCESSION   DASRKK010000240 DASRKK010000000
VERSION     DASRKK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310652"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1334
                     /locus_tag="VFC15_05265"
     CDS             270..1334
                     /locus_tag="VFC15_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM09602.1"
                     /translation="MSEKLNTAIAVIGIDIGKNSFHVVGHDERGAIVLRQKWSRGQVA
                     ARFANMPPCLIGMEACVGAHHLSRKLKAFGHDARLMPAKYVRPYSKGQKNDFRDAEAI
                     AEAVQRPTMKFVATKTADQLDLQALHRVRERFVSQRTGIINQIRAFLLERGIAVRQGQ
                     RFLRAELPRILATPSDVLSPRMVHLIENLAGDWRRLDERIESLSSEIEAIARQDAGCE
                     RLMSVPGIGPIISSAMVAAIGSGEVFTKGRDFAAWLGLVPRQMSTGGRTIRGRISKRG
                     NRYLRVLFVQAAWVVLIKPKSWERHGLKSWIEAAKKRLHPNVLAIALANKLARIAWGV
                     LAHGRDFEVRKISEAAAQSA"
BASE COUNT          556 a          772 c          748 g          483 t
ORIGIN      
        1 ttcttggatt ggctcgtggg cgtcatcacg ctgaacgagc tcacaagatc aactcgtaaa
       61 cgggaaaagc gttccggatt caaattgagt tccatcaaag cagagtcggc acaggccagt
      121 acccagcaac gtatagaagc agatcggaaa cttggggcct ttgctgaatg ccactcagcc
      181 ataaggtccg ctaatgggat ggtccggccg tgctcctgcc ccgccggcat tttaagctgg
      241 ccaggggcac caaagacaag gagcacacca tgtctgagaa actcaatacc gcaatcgccg
      301 taatcggcat cgatatcggc aagaactcgt tccacgtcgt gggccacgat gagcgtggtg
      361 cgatcgtgct gcgacagaag tggtcgcgtg gccaggtagc cgcacgcttt gccaacatgc
      421 cgccgtgcct gatcggcatg gaggcctgcg tcggtgcgca tcatctcagt cgcaagctta
      481 aggcgtttgg ccacgatgct cgtctgatgc cggcgaagta cgtgcgcccg tactcgaagg
      541 gacagaagaa tgacttccgc gatgcggagg ctattgccga ggcggtgcaa cgcccgacga
      601 tgaagttcgt ggcgacgaag accgccgatc agctcgacct gcaggcgctg caccgggtgc
      661 gggagcggtt tgtcagccag cgcaccggca tcatcaatca gatccgcgct ttcctgctgg
      721 agcgcggcat tgcggtgcgg cagggacaac gcttcttgcg tgccgagctg ccgcgcatcc
      781 ttgccacacc ctccgacgtc ctttcgccac gcatggtgca cctgatcgag aacctggccg
      841 gcgactggcg ccggctcgat gagcggatcg aaagtctgtc gagtgagatc gaagccatcg
      901 cacgccagga tgccggttgc gagcgcctga tgagtgtgcc cggcattggg ccgatcatct
      961 ccagcgcgat ggtggccgcg atcggctctg gagaagtctt caccaaaggc cgcgacttcg
     1021 ccgcatggtt gggtctggtg ccgagacaga tgtcgaccgg gggccgcacg atccggggca
     1081 ggatctccaa acgcggcaat cgctatctac gcgtcctgtt cgtgcaggcg gcgtgggttg
     1141 tgctgatcaa gccaaagagc tgggagcgcc acgggctcaa atcctggatc gaggcggcca
     1201 agaagcggct gcaccccaac gtgctcgcga ttgcgctcgc caacaagctg gcccgcatcg
     1261 cttggggcgt tctggctcat ggccgggact tcgaagtaag gaaaatcagt gaggccgccg
     1321 cccagtcggc ttgatcttga ttctacaccg aggaacgcag cgagcgcgca aagtgaggag
     1381 cctacgatga cgtagccaga tcgatacacc ttacctgccg aggtctgcga gaggatgaaa
     1441 cgagatggag gttcggtctt cccggcgcat gcgaacactg atgacccaaa tggcccgctc
     1501 gaggcctttc cgctaatgag atcgcacgcg cgctgatatc catgatggcc cggagcacaa
     1561 cacgctccaa tcagaggccg gatacattga tgcaagaccg ctcaccgcca gctcggcaaa
     1621 atccacttgc aacgcacggc cggaccatac attcgggtca ttcacgaccg atggattcag
     1681 cgccagtttc ggccaatgtc cgctgtccat ccgatagcga ccgtggcgct gcaatgcagc
     1741 gaacgacgct ttgtgccatt agcgacatgg cggtccaagt aatggcttcg accattcatg
     1801 gcactcacgt gccggtggag gagccgtcca cagcatcaat aggcgacatc gcacacttag
     1861 ttaaaaatga aagaggccgc gaactgaggc ggccaaaaca agaagccccg gccaccacca
     1921 tgacggtgac aaccggggtt tcccagtgcg gaagcgaccc taaaggggca aaccaaaagg
     1981 gccgaaaccc ggcacctttc gtatgtgcct gaattttaat catcaatcaa actaggccac
     2041 tccagttttc aaatcaggcc agtacctggc gtgtcagacg cggcccttcg gtccgggaag
     2101 attggaccaa tcaaggccga gcgcttcgca gacttggcga accgaacgcg gatcgagctt
     2161 atcgcttgca ccgaccagcg acagttctgc agtccgccct gcgcggtgga ttgtcactgt
     2221 cccatgacct tctccgcgct tttcgggttg cgtatcgaag cgacatccct gttcggccag
     2281 ccactttcgg aacgttgcgt tttccatcga tcctctccgg tcttcgccct aacgatgcaa
     2341 ccaagatagc ccaaattcaa aagggaccac tcatgcttga ggggggtcga aaggggccag
     2401 tacccggcgc tggtagagtc ctaatttgaa ttcagccgcc aatcttgctc agacgctcag
     2461 tcggtggctt gcacatgacc gtgctgccat cgtgatcgcg cttactctcg cccttcaagc
     2521 cagcaacacg ccacttgcac acgtcctccg ctatccatg
//
LOCUS       DASRKK010000241         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_115185, whole genome shotgun sequence.
ACCESSION   DASRKK010000241 DASRKK010000000
VERSION     DASRKK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115185"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(586..1935)
                     /locus_tag="VFC15_05270"
     CDS             complement(586..1935)
                     /locus_tag="VFC15_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM09603.1"
                     /translation="MAFQPIDRDTDFLLPPSVQEWLPQAHLARYVVDVVEGLDLSALE
                     RMYAGRGSDAYHPATLLSLLIYGYATGTFSSRKIERATYDSLAFRYIACNRHPDHDTL
                     ATFRTRFGKEFESAFVQVLQVARENQLSRFGTVSLDGTKIHANASRHSALSYGHCEKI
                     EAHLNAEVQELLALAEAADQSAVPDGVNLPAEITRREDRLAAIEAAKVKIEARAKERF
                     EGEQADYEAKMAARTAKAEAGTKPRGKEPKPPESGPRANDQINLTDEESRIMKIAGGF
                     EQCYNAQAVVDTESMLVLATHVTQAANDKEQVEPMVAKIRANPEGFNQPETWLADTGY
                     YSAKNVAVCLAAKIDPLIAVKRDEHHPDWRDRFTEPLPLTDEASPVEIMKHRLKTRVR
                     RAAYALRKQTVEPVFGIIKSVMGFRQFLLRGLDNVKNEWTLVCLAWNLKRMAVLRLK"
BASE COUNT          440 a          624 c          580 g          463 t
ORIGIN      
        1 gaagaagggt gttccactga tgattctgga agccatgaag atgcacaacc ggatcgcttc
       61 gccgctggat ggtaccgtca aggccatccg cgtcgcgccc ggtgaaaagg tttcgaaggg
      121 ccagattctg atcgaaatcg aatagccgca tcgcacgatc gtcgcggacg attccgaccg
      181 cgacacaaag acgccggcga gacgtggagc acacgtcccg ccggcgtttt ctcattcaaa
      241 tgtcacccca ttacgccaga tggcgaaagc cggggactcc ttgcaacaag ccatcaatgg
      301 gccacgacca gatcttcggt actgaagaac tggcctgctg acgattattc gctttctcca
      361 tgaaccgcgt cattgcccaa ttgcgcaacc atcaacaaat gattcgatct ctcagaccgc
      421 gctctatcgg tatctcatga atggcggcag ttcaagagca tctagcagcc cgtcggactt
      481 gaacacgagc agcggtcaga tttcacgatc tgggaataat tgacactttt ttagcggaaa
      541 tcaggtcgat tattcacgga attacaatgc tgatgtccta ttttcttact ttaggcgcaa
      601 tacggccatg cgcttcaaat tccacgcgag gcaaaccagc gtccattcgt tcttgacatt
      661 atccaggcca cgcagcagaa actgtcggaa gcccatcacc gacttgatga tgccgaacac
      721 cggttcgacc gtctgcttgc gtagcgcata ggcggcacgc ctgactcggg tcttgaggcg
      781 gtgtttcatg atctcgaccg gggaggcctc gtccgtcagc ggcagcggct cggtaaaccg
      841 gtctcgccag tccggatggt gttcgtcgcg cttgacggcg atcagcggat caatcttggc
      901 ggcaaggcat accgccacat tctttgcact gtaataaccg gtgtcggcca gccaggtctc
      961 gggctgattg aacccttcgg gattggcacg aatcttcgcc accatcggct ccacttgttc
     1021 cttgtcgttc gcggcttgag tgacgtgcgt ggcgaggacg agcatggatt cggtgtcaac
     1081 gaccgcttgg gcgttgtagc actgctcaaa gccgccagcg atcttcataa tccgcgactc
     1141 ctcgtcggtc aggttgatct gatcgttggc tcgcgggcca gactcgggtg gcttcggctc
     1201 cttgccgcgg ggcttcgtgc cggcctctgc cttggctgtg cgggcggcca ttttggcctc
     1261 gtaatcggcc tgctcgccct caaaccgctc cttggcgcgc gcttcaatct tgaccttcgc
     1321 ggcctcaatc gcggccaggc gatcttcccg gcgcgtgatc tcggcgggaa ggtttactcc
     1381 atccggcacc gccgactggt cagccgcttc ggccaaggcc aacagctctt gcacctcggc
     1441 gttcagatgg gcttcgatct tctcgcagtg tccgtacgac aaggcgctgt gccggctggc
     1501 attggcgtga atcttggtgc cgtccaggct caccgtacca aagcgcgaga gctggttctc
     1561 acgggccacc tgaagaactt gaacaaacgc cgattcgaat tccttgccaa agcgcgtcct
     1621 gaacgtcgcc aaggtgtcgt gatccggatg ccggttgcag gcgatatacc ggaacgccag
     1681 tgagtcgtag gtcgcccgct cgatcttgcg actggagaac gtcccggtgg cgtaaccgta
     1741 aatcagcaag gacagcagcg tcgctggatg gtacgcatca ctaccccggc ccgcatacat
     1801 ccgctccagc gccgacaggt caagcccttc aaccacatca acgacgtagc gcgccaggtg
     1861 cgcctggggt aaccactcct gcaccgaggg cggtagcaag aagtccgtat ctcgatcgat
     1921 cggctgaaag gccattacgg tgtccgtcaa agtaacaatt atgattatcg catatttcaa
     1981 gaaaagcccg acaaactgct agggggtcac gattcagcgt ccatgacaaa atagcacctt
     2041 gatgacaaaa ggattcgttg taagatttcc ttgtttcaag acgctttcct ttgtgactga
     2101 catcctg
//
LOCUS       DASRKK010000242         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1386085, whole genome shotgun sequence.
ACCESSION   DASRKK010000242 DASRKK010000000
VERSION     DASRKK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386085"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1930
                     /locus_tag="VFC15_05275"
     CDS             485..1930
                     /locus_tag="VFC15_05275"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM09604.1"
                     /translation="MTGPEPEGDHQRIEDLEEENQSLREELTRVRTENDRLREEVKKL
                     GKLLRSKARSATPFSKGKRKAHPKRPGRKPGQGPFQRRDAPPAVAGADPVEVPVTNMR
                     CPCCAGELKWQRTDRVTTTDIPAQPQPEVKVYDVAVCVCTVCGKSVRGKHSAVAADQY
                     GATAHRVGPRLKTMAHVLHYGYGVPVRRLPAILLATTGVTLTQSAMTRDALKQASGVV
                     GNAYQEARAGIRHAPVTYTDDTSWAVGGEPAQLMVFDTDQATVYQIRPQHRNQEVREL
                     VPGDYQGTLVTDRGSSYEADELAGVDQQKCLAHLMRNLKEVVETKSGQACKFGEGVLN
                     TLKQANQLWRDHRAGKVDREGFRLQRQEIEDQLTHQLRHRQLTDPDNQRLLDGIGLQN
                     DRGRVLRFLHNPGIEPTNNRAERALRPAVIARKVSQCSKTQRGADAFAAFTTVIRTIA
                     KKAETSIAQGLHSLLHPAVSASGSTPPQNSG"
BASE COUNT          479 a          628 c          637 g          316 t
ORIGIN      
        1 ggagcattct gcgccaacgt caaaagccac gccggaatca acgaagccga agcggacgct
       61 cagcgccgct ggcaaggccg caattgtcgc tgcattgaag aagcgttggg cggcgttcca
      121 tgcggcaaat caagcgaaga aggcggccaa ggcctagcag ccacccgccg ccaagaaagc
      181 cgttgccaag aaagcgccgg tgaagactgc aaagaaggcc gtgaggaagg ttgccgccaa
      241 gcgaccgcag aaagccgccg cgactcctgc tgttctggca gcgaccgagt agcgctgcca
      301 aatcggcgac ccgcccgcct atggggcact catagcttcc agcacagttc acgaaagcgg
      361 ctcaggaatc gggcctttgc cagtacctga ttcccggcat tgcctggcag gctcggtcgt
      421 agcagactga cggtcggaaa gaaatctctg gacattcgga gcgggatcgg ttaccgtagt
      481 ctggatgact ggaccggagc cggaaggcga tcaccaacgg atcgaggatt tggaagaaga
      541 aaaccaatcc ctccgcgagg agttgactcg ggtccggacc gagaatgacc ggttgcgcga
      601 agaagtgaaa aagctgggaa agcttctgcg gagcaaagcg cggtcggcca cgccattttc
      661 caaaggcaaa cgcaaagccc accccaaacg ccccggccgg aagcccggac agggaccctt
      721 ccagcgccgc gatgccccac cggcggtcgc cggggccgac ccggtcgagg tgccagtgac
      781 gaacatgcgc tgcccctgct gcgcgggaga gttgaaatgg cagcggaccg accgggtgac
      841 caccacggac ataccggctc agccccaacc ggaagtgaaa gtctatgacg tggccgtgtg
      901 cgtgtgtacg gtttgcggaa agagtgtgcg ggggaagcat tccgcagtag ctgcggacca
      961 atacggggcg accgcgcacc gggtgggtcc cagactgaag accatggcgc acgtgctgca
     1021 ctatggctac ggggtgcccg tgcgccggct accggccatt ctactggcga cgaccggggt
     1081 aacgctgacg cagagcgcca tgacgcggga cgccctcaag caagcctccg gcgtggtggg
     1141 caatgcctat caggaggccc gcgctgggat ccgccacgca ccagtcacct acaccgacga
     1201 caccagttgg gcagtaggtg gtgagccagc ccagttgatg gtgttcgaca ccgaccaggc
     1261 caccgtctac caaatccgcc cccagcatcg caaccaagag gtgcgggaac tggtgccggg
     1321 cgactaccaa ggcactttgg taacggatcg aggttccagc tacgaagccg acgagttggc
     1381 cggagtggat caacaaaagt gcctcgcgca tctgatgcgc aatctcaaag aagtcgtgga
     1441 aaccaaaagt ggacaggcgt gcaagtttgg ggaaggtgta ctgaacaccc tgaagcaagc
     1501 caaccagttg tggcgcgacc atcgggcggg caaggtggac cgggaaggct ttcggctgca
     1561 aaggcaggag atcgaagacc aactgaccca tcaattgcgc catcgccagt taacagaccc
     1621 cgataaccag cggttgctgg atggcatcgg cttgcagaat gatcggggcc gggtcttgcg
     1681 attcctccac aacccgggca ttgagcccac caacaatcga gcggaacggg cactccggcc
     1741 agcggtgatc gcccgtaaag tttcgcagtg ctccaaaacg cagcgcggcg ccgacgcatt
     1801 tgcggctttc accaccgtaa ttcgaaccat agccaagaag gccgagactt ccattgctca
     1861 aggacttcat tccttgctgc atcccgcagt ttccgcctca ggctccactc ctccccagaa
     1921 ctccggctaa gcacgacaga tccactgcaa ctccaacgat ccgcttgaac tcgaccgcga
     1981 atcaactacg agcggggtag tgctgtaggt gttccacggg acgccgttct tgccggctac
     2041 gacaaatcca ttggcagggt
//
LOCUS       DASRKK010000243         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_702090, whole genome shotgun sequence.
ACCESSION   DASRKK010000243 DASRKK010000000
VERSION     DASRKK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702090"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2258
                     /locus_tag="VFC15_05280"
     CDS             <1..>2258
                     /locus_tag="VFC15_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     serine/threonine-protein kinase"
                     /protein_id="HZM09605.1"
                     /translation="REARAASSLDHPNICAIYEFGEHEGRPFIVMQLLQGQTLRDCLA
                     SGALKDTASGAPVALDRLLDIAIQIANGLEAAHEQGIVHRDIKPANIFITTKGVAKIL
                     DFGLAKLLQPSSEQAIAAEIDGESRTPLPAIAGPEALNISRPGIAVGTAAYMSPEQAR
                     GQQLDARTDLFSFGLILYEMGTGRRAFTGDNAAAMHDAILNRAPTPPAELNTELPTEL
                     QDVIQKCLEKDRNLRYQKAAEIRSDLEKVKRRREHPLLSRWRLLATAAVIVVALLAGG
                     LYWRSRKTTTLTEKDTIVLADFVNTTGDSVFDDTLKQGLSVQLEQSPFLALVSERKVN
                     ETLKLMGRPAGDRLTPEVAREVCQRTGSAAMLTGSIAGLGSQYVIGLRAVNCSTGDVL
                     AEAQEQAAGKEAVLKALSAAAVSLRGNLGESLSSVQKYATPLEEATTPSLEALKAYSM
                     GRKAKFAKGDTAALPFFKRAVEVDPGFALAYQAMSIVYGDLNEAGRAAENAHKAYEQR
                     EKVSERERFAIEASYYSDSTGELEKAAQVYELWQQTYPRDATPYNRLGGDISIPLGNW
                     EKALEEGREAIRLEPNSEANYANLGLDYASLNRLDEAEAVYKQAKERKLEGEYLLANR
                     YQLAFLKGDAAQMAQLVAAAMGKPGGEDWFLAAQADSEAWYGKLKNARELTRRAMDSA
                     QHNDANEAAAAYQAAAALREVESGNREQARTDAEAAVKLAPNRNVRALATLALARAGD
                     TAAAEKLAAELD"
BASE COUNT          493 a          643 c          749 g          373 t
ORIGIN      
        1 cgcgaggccc gcgccgcttc gtcgttggac catcctaata tttgcgccat ctacgaattc
       61 ggagagcacg aaggccggcc cttcatcgtg atgcagttgc tgcaagggca aaccttgagg
      121 gactgcctgg cctcaggcgc gctgaaggac acggcttcgg gagcgccggt ggccctcgac
      181 cggttactgg atatcgcgat ccaaattgcc aacgggctgg aagctgcgca tgaacaaggc
      241 atcgttcacc gcgacatcaa gccagccaac attttcatca ctaccaaggg cgttgccaag
      301 attctggatt tcggtttggc aaagctgctc cagcccagca gcgagcaagc gattgcggcc
      361 gaaatagatg gcgaatccag gactccgttg ccggctatcg caggcccgga agccttgaac
      421 atcagccgcc ccggtatagc tgttggtact gcagcgtaca tgtcgccgga acaagcgcga
      481 gggcagcagt tggacgcgcg cacggacctc ttttcctttg gcttgatttt gtatgaaatg
      541 ggaaccgggc ggcgggcatt caccggcgac aacgcggcgg cgatgcacga tgccatcttg
      601 aatcgtgcgc cgacgcctcc cgcggagctt aataccgaac tgcccactga gttgcaagac
      661 gtcatccaga agtgcctgga gaaggaccgc aacctgcgct atcagaaagc cgcggagatc
      721 cgctccgact tggagaaggt caagcgtcgc agggagcatc ccctgctcag ccggtggagg
      781 ctgctggcga cagccgcggt catcgtggtc gccctgcttg ccggcggtct ttactggcgc
      841 tcgcggaaga ccaccacgtt gacggagaaa gacaccatcg ttctcgccga cttcgtcaac
      901 actaccggcg acagcgtttt cgacgacacg ttgaagcagg gactctcggt acagcttgag
      961 caatcaccct tcctcgccct ggtttccgag cgcaaggtga acgagacttt gaagctgatg
     1021 ggacgtcccg caggtgaccg tttaacgccg gaagtggcac gcgaggtctg ccagcgtaca
     1081 ggcagcgcgg ccatgctgac cggctcgatt gccgggctgg gcagccagta tgtaattggt
     1141 ctgagggcgg tgaactgtag tacgggagac gtgctggcgg aagcgcagga gcaggcagcg
     1201 ggcaaggaag cggtgcttaa ggctctgagc gccgcagccg tcagcttgcg cggcaacctg
     1261 ggcgaatcgc taagctcggt acagaagtac gctactccgc tagaggaagc gaccacgcca
     1321 tcgctggagg cgttgaaagc ctacagcatg ggacggaagg cgaagtttgc gaaaggggac
     1381 actgccgctc tgcccttctt caaacgggcg gtggaagtcg atccgggttt cgccctggcc
     1441 taccaagcca tgtcgattgt ctacggcgac ctcaacgaag ccgggcgggc agcggagaat
     1501 gcacacaaag cctatgagca gcgggagaag gtgagcgaac gcgagcggtt cgctatcgag
     1561 gcgagctatt actcggactc gaccggagag ctggagaagg cggcgcaggt gtacgaactg
     1621 tggcagcaaa cctatccgag agatgccacg ccttacaacc gcttgggagg agacatttcc
     1681 atcccccttg gaaactggga aaaggcgctg gaggaaggac gagaggcaat acgcctggaa
     1741 ccgaacagtg aggccaacta cgccaacctc ggcctcgact acgcgagcct caaccggcta
     1801 gacgaggcgg aggcggtgta caagcaggca aaggagcgca agctggaggg tgagtacctg
     1861 ctcgcgaatc gctaccagtt ggcttttctg aagggggatg cggcgcagat ggcgcaactg
     1921 gtcgcggctg ccatgggcaa gccgggtggg gaagactggt ttctggccgc ccaagcggac
     1981 tcggaggctt ggtacgggaa gctgaaaaac gctcgcgagt tgacccggcg ggcgatggat
     2041 tcagcccagc acaatgatgc caatgaggcc gccgcagcgt atcaggcagc ggcggcgctg
     2101 cgcgaggtgg aatcgggcaa ccgggagcag gcacgtaccg atgccgaggc agcagtgaag
     2161 ctggccccaa accgcaatgt acgggccttg gcgacactgg ctctggcgcg ggcaggcgat
     2221 acggcagcgg cggagaagct ggctgccgaa ctcgacaa
//
LOCUS       DASRKK010000244         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_799881, whole genome shotgun sequence.
ACCESSION   DASRKK010000244 DASRKK010000000
VERSION     DASRKK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799881"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFC15_05285"
     CDS             <1..998
                     /locus_tag="VFC15_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09606.1"
                     /translation="QKPIEGTSFAYTFDAAGAKAPSQHKTQYFEMMGQWALYHEGWLL
                     STKPDRAPWDVFVSPNPDPLNNQVFQLYDLSQSWNQYEDIAAQHPDKVKQMREMFVEE
                     AKKYQVFPLDASVVQRIATPKPSLTAGLSELVYTRPMTGVPQGDAPYLLNTSYTLTAD
                     ITVPEGGAEGMIATSGGRFAGWGFYLLKGKPVFCWNLLDLEWIKWEGPQALAPGRHTI
                     EFDFKYDGLGIATFAFNNFSGVGKGGTGTLKVDGKVVDTKRMEKTIPIILQWDESFDI
                     GSDTITGVNDADYLPPFPLTAKFNKLTIKIDRPQLSPEDIKKLEEGMKQAALGRQ"
     gene            1164..2027
                     /locus_tag="VFC15_05290"
     CDS             1164..2027
                     /locus_tag="VFC15_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09607.1"
                     /translation="MNRNHIAGALVFIVLCGLHATAQQEAAAPTADGDSGSQKDQPEP
                     SIEEIRKLKQNPLSALRSLYLQNTNGTVGGESADVFSLQPVWPFRVGDKCRLITYTII
                     PITWAPAVTPGENSASGLGNILFNGFFRPEKEGPLTWGVGPAIELPTRTNPRLGSDRV
                     SLGPAIILFGSSGSWTGGVVVQNYWSLGGSGSNKVNQFGLQYIVNYNLPKGWYLLSNA
                     TITANWLAASQNRWLVPLGGGPGRVFKVGKSKQSYAAAIQGFRNVASPSLGPNWEVIA
                     QFQIIFSMPGT"
     gene            2063..3571
                     /locus_tag="VFC15_05295"
     CDS             2063..3571
                     /locus_tag="VFC15_05295"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM09608.1"
                     /translation="MKSYRHFSIFLLLSVLLIATAAIPVSGQYKNSTPIPPSVPAPDK
                     IDTPFGTLHLTAGFPDKDSTQKLYDNLDFQHAVQAYLLGLPPVSQWANRKGMLAWGPP
                     NTTVPIFETMMNAKTLELTANNNTPYTWFWLDLHAGPLVVEVPPKVLGLIDDMWYHWV
                     ADVGITGEDKGAGGKYLLLPPDYKGDVPQGYFVVRPKTFSIWIPWRTFLVDGSPAPGV
                     DMVKKFTKIYPLSQAGNPPALNFVNVSDKAFNTLAPADYSFWEALNQVVQEEPTDPID
                     PTTLGFWASVGIQKGKPFAPDARMKKILTEAAQVGDATARAIFYRWRGADGYYYPNSA
                     WRIGFIGGYKFEENGARLLDAYESFFFYATGITPAMDTREVGIGSQYMCAFVDSKGDP
                     LEGGKTYRLHLPKDIPVKDFWSVILYDSQTRSMLQTNQQWPAVSSQTKGLQVNPDGSV
                     DVYFGPKAPAGKESNWVQTIPGKGWNTLLRLYGPLQPWFDKTWRPGEIELQP"
     gene            3666..5207
                     /locus_tag="VFC15_05300"
     CDS             3666..5207
                     /locus_tag="VFC15_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM09609.1"
                     /translation="MRAFRQFLFYGTLAATIAITPATTVAQTPSGSSSVPPKYAPDVP
                     AKITTPDTVTTRIGTLRFKDGAPDAATAQLAYDQLDFGRGVDAFMKGMSATSVHAMCR
                     GFEQAGIKLNQGIGITADLFDARSLFLTANTTTVYVVLCIDLKDGPMVMRVPPRVLGP
                     VDDADFRWVTDVGLTGPDKGAGGDYLFVPPGYKGALPAQGYHIANPRTNRLLVFYRAF
                     VEKGDIAAAVAGVKAKAGVFPLSRAAAPPATGVVNISGLKFNTISANDFSYYEELNAV
                     VQNEPADWVDPDAVGLYGAIGIRKGQPFAPDARMKGILTDAVAVANAIARTNLYASRD
                     PKTRIYTDRQWWTPFVGGSYQFLNGAERLLDARMMFFYYATGITPAMTESRPGTGSAY
                     AITVRDATGSYLDPNRTYKVTLPGPIPAKDFWSFTVYDNQTRSLLPTDQKLAGLDSTL
                     SGIRMNPDGGVTIWFGPKAPAGQEKNWVQTMPGTGYNVALRLYGPLEPWFNQTWRPGD
                     IELQP"
BASE COUNT         1179 a         1719 c         1643 g         1019 t
ORIGIN      
        1 agcagaagcc catcgagggc accagcttcg cctatacctt cgacgccgcc ggcgccaagg
       61 cgccgtcgca gcacaagacg caatacttcg agatgatggg ccaatgggcg ctctatcacg
      121 aaggctggct cttgagcacc aagcccgacc gcgcgccgtg ggacgtgttc gtttccccca
      181 acccggaccc gctcaacaac caggtgttcc agctctacga cctcagccag agctggaacc
      241 agtacgagga catcgccgcg cagcatccgg acaaggtcaa gcagatgcgg gagatgttcg
      301 tcgaggaagc gaagaagtat caagtcttcc cgctggacgc gtccgtggta cagagaattg
      361 cgactccgaa gccgagtctc actgccggcc tcagcgagtt ggtctacacg cggccgatga
      421 caggcgttcc gcagggcgat gcgccgtacc tgctcaacac ctcctacacc ctcacggcgg
      481 acatcaccgt gccggaaggc ggcgccgagg gcatgatcgc gacttccggc gggcgtttcg
      541 cgggctgggg cttctacctg ctcaagggca agccggtgtt ctgctggaac ctgctcgatc
      601 tcgagtggat caagtgggaa ggtccgcagg cgctggcgcc tggccgtcac accatcgagt
      661 tcgacttcaa atatgacggc ctcggtatcg ccacgtttgc gttcaacaac ttttccggcg
      721 tcggtaaggg cggcaccggc acactgaagg tggacggcaa ggtggtcgat acgaaacgga
      781 tggagaaaac gatccccatc atcctgcagt gggacgagag cttcgatatc ggctcggaca
      841 cgatcaccgg cgtcaacgac gccgactatc tgccgccgtt cccgctgacg gcgaagttca
      901 acaagctgac gatcaagatc gaccgcccgc agctatcgcc cgaggacatc aagaaattgg
      961 aggagggcat gaaacaagcc gcactcggcc gccaataacg tcatttcggt aatgacccgc
     1021 gggtcgtgcc ggacactttc ggcacggccc gcgactcgca ataatcgcgc aagcgaataa
     1081 ctagtgctcg acaaatgggg cagctcgcaa gagccgtccc ttcgcaaaac atttcgagaa
     1141 gacaacgcca agcaaagaac cctatgaatc gaaaccatat tgcgggtgcc ttggtcttta
     1201 ttgtcctctg cggactgcat gcaacggcgc agcaagaggc cgctgcgccc accgcggacg
     1261 gagactccgg ctcacagaag gatcagccgg agccatcgat cgaggaaatc cgaaagctca
     1321 aacagaaccc gctaagcgcc ttgcgcagcc tttatctcca gaacaccaat ggcaccgtcg
     1381 gcggcgaatc agcagacgtt ttctcgctcc agcccgtatg gcccttccgg gtaggcgata
     1441 aatgcaggct cattacctac accataattc cgatcacctg ggcaccggcg gtaacgccgg
     1501 gagaaaattc ggcctcggga ttaggcaaca tactgttcaa cggctttttc agacccgaaa
     1561 aagaaggtcc gctcacgtgg ggtgttggcc ccgccatcga gctgccgacg cgaacgaatc
     1621 ccaggctggg cagcgaccgc gtgagcctcg gtccggcaat catacttttc ggaagttcag
     1681 ggtcctggac cggtggtgtc gtcgttcaga actattggtc gttaggcggc tccggaagca
     1741 acaaagtcaa tcagttcggc cttcaataca tcgtcaacta caacctgcct aaaggttggt
     1801 atctgcttag caatgccacc ataacggcaa actggttggc ggcctcacag aatcggtggt
     1861 tggtaccgct cggcggcggg cctggcaggg tattcaaggt cggcaaaagc aagcagtctt
     1921 atgccgccgc gatccagggc tttcgtaacg tcgccagtcc gagcctgggg ccgaactggg
     1981 aggtgatcgc tcagttccag atcatctttt ccatgcccgg gacgtagaaa cagggactgg
     2041 atttacacat atggaggatt cgatgaaatc gtaccgacac ttttcaatat tcctgttgtt
     2101 gtccgtactc ttgatcgcca ctgcggccat cccggtcagc gggcagtaca agaactcaac
     2161 cccaatcccg ccaagcgttc ccgcgccgga caagattgat acgccgttcg gcacgctgca
     2221 tctcaccgcg ggtttccctg acaaggactc gacccagaag ctctacgaca acttggattt
     2281 ccagcacgcc gtgcaggcct atctgctcgg cctccctccg gtgagccagt gggcaaaccg
     2341 taagggaatg ctggcgtggg ggccacccaa tacgacggtg ccgatctttg aaaccatgat
     2401 gaacgccaag acactggagc tgacggcgaa caacaacacg ccttacacct ggttctggct
     2461 tgacctgcac gctggtccgc tggtggtaga agtgccgccc aaagtactcg gtcttatcga
     2521 tgacatgtgg taccactggg tcgctgatgt cggtatcacc ggagaggaca agggcgcggg
     2581 cggaaagtac ttgctgctgc ctcccgatta caagggcgat gtgccacagg gctacttcgt
     2641 cgtacggccg aagaccttca gcatctggat tccgtggcgt acctttctgg tcgacggtag
     2701 cccggcgccg ggcgtggaca tggtgaagaa attcaccaag atctaccccc tgtcgcaagc
     2761 cggaaatcca ccagctctta actttgtaaa cgtctccgac aaggcgttca acaccctcgc
     2821 gccggctgat tactccttct gggaggctct caatcaggtc gtccaggagg aaccgacgga
     2881 cccgatcgat ccaaccacgc tcggcttctg ggcgtccgtc ggcatccaga agggaaagcc
     2941 gttcgcgccc gacgcccgca tgaagaagat cctcaccgaa gcggcccagg tgggtgacgc
     3001 cacagcccgc gccatctttt accgctggcg gggcgctgac ggctactact acccgaacag
     3061 tgcctggcgc atcgggttca ttggcggcta caagttcgag gagaacggcg cccgtctgct
     3121 ggacgcatac gagagcttct tcttttatgc aaccggcatc actcccgcaa tggatacgcg
     3181 ggaggtaggc atcgggtcgc agtacatgtg tgcgttcgtt gactccaagg gagatccgtt
     3241 ggagggcggc aagacctatc gcctgcacct gccgaaggat atcccggtaa aagacttctg
     3301 gtcggtcatt ctgtatgaca gccagacccg ctcaatgctg cagaccaatc agcagtggcc
     3361 ggcagtcagc agccagacca agggactgca ggtaaatccc gatggttcgg tggatgtgta
     3421 cttcggaccc aaagctcctg ctggtaagga aagcaactgg gttcagacca tccccggtaa
     3481 gggctggaac accctgttgc gtctctacgg cccgctgcaa ccgtggttcg acaagacctg
     3541 gcggccgggc gagatcgagc ttcaaccgtg attggccaac gctgttcttg ggccgcatcg
     3601 cgcggagcaa ctcaactgga ccaagtctga aactttcata aactgatgcg agaaggaagc
     3661 aaaaaatgag agcatttcgg cagttcctgt tctacggaac cttggctgcg acgatcgcaa
     3721 tcacgccggc tacaactgtc gcacagacgc ccagcggctc gtcgagtgtt ccaccgaagt
     3781 acgcgcccga cgtgcccgcg aagatcacca cgcccgacac cgtgacgacg cggatcggca
     3841 cactgcgctt caaggacggc gcgcccgatg ccgcgacggc gcaactggcc tacgaccagc
     3901 tagacttcgg ccgtggcgtc gatgcgttca tgaaggggat gtcggccacc tcggttcatg
     3961 cgatgtgccg gggcttcgag caggcgggca tcaagctcaa ccagggcatc ggcatcaccg
     4021 cggacttgtt cgatgcccgc tcgctgttcc tgaccgctaa cacgactacc gtctacgtcg
     4081 tgctctgcat cgacctgaag gacggaccga tggtcatgcg ggttccgccg cgggtcctgg
     4141 gcccggtcga cgacgccgac ttccgctggg tgaccgatgt cggactcacc ggcccggaca
     4201 agggcgcggg tggcgactac ctgttcgtcc cgcccggcta caagggtgcg ctgccggcgc
     4261 agggctatca catcgccaat ccgcgcacca accggctgct ggtcttctat cgcgcgttcg
     4321 tggagaaggg agacatcgcg gcggccgtcg ccggcgtcaa ggcgaaggca ggcgtgttcc
     4381 cgctgtcccg ggccgccgca ccaccggcca ccggggtcgt caacatctcg ggcctcaagt
     4441 tcaacaccat cagcgccaac gacttcagct actacgagga gctgaacgca gtggtgcaga
     4501 acgagcctgc ggactgggtc gatccggatg ccgtgggact gtacggcgca atcggcatcc
     4561 gcaaggggca gccgttcgca cccgacgcgc ggatgaaggg gatcctgacg gacgcggtgg
     4621 cggtggcgaa tgccattgcg cgcacaaacc tgtacgcctc gcgagatccg aagacccgga
     4681 tctacaccga ccggcaatgg tggacaccgt tcgtgggcgg cagctatcag ttcctcaacg
     4741 gcgccgagcg actgctcgat gcgcgcatga tgttcttcta ctacgccacg ggcatcacgc
     4801 cggcgatgac cgagtcgagg ccgggcaccg gttccgccta cgcgatcacc gtgcgcgacg
     4861 cgaccggaag ctacctggac ccgaaccgga cctacaaggt gacattgccg gggccgatcc
     4921 ccgcgaagga cttctggtcc ttcacggtct acgacaacca gacccgttcg ctgctgccga
     4981 ccgaccagaa gctggcggga ctcgacagca cgctctcagg catccggatg aacccggacg
     5041 gcggcgtcac catctggttc ggccccaagg caccggccgg ccaggagaag aactgggttc
     5101 agaccatgcc cggcacgggc tacaacgtcg ccctgcgcct ctatggcccg ctggagccgt
     5161 ggttcaacca gacctggcgg cccggtgata tcgaattgca gccctgatcc attcgttacg
     5221 aaagtgagag taaatatgaa tggacgtgaa tcgaatctga agacgctcgg gctagctgtt
     5281 ctacctgtcg gcgttcgcaa cgctcgcggt gttgttctgg ccgtacatga ttccgtattc
     5341 catcaccgtg ggcaatgcgg ccgcgcccga ggcgtcgctc tctttcctgt tctggcttgt
     5401 tcgtattgcc tgtcatcgcg atctacacca ttgccgtcta ctggttcttc cgcggcaaga
     5461 tgctgagggg gacggctgag cacaagcttc gagaagaagg actagtccgg aaggattcgt
     5521 caagcaactg atgcagaaga tctattagct taaccaacgg
//
LOCUS       DASRKK010000245         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_603989, whole genome shotgun sequence.
ACCESSION   DASRKK010000245 DASRKK010000000
VERSION     DASRKK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603989"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VFC15_05305"
     CDS             complement(<1..1811)
                     /locus_tag="VFC15_05305"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZM09610.1"
                     /translation="MSKKGTTSNSGKRKQEKGSARNGDSQVAAGGELHQIAGGEHPAL
                     TTNQGVALSDNQNSLRANPHGPTLLEDFILREKITHFDHERIPERIVHARATGVHGFF
                     ELTASLKQYTTARILTEVGEKTPVFTRISTVAGGAGSVDTPRDIRGFAVKFYTKEGNW
                     DLVGINIPVFFIQDAIKFPDLIHAVKMEPDRGFPQSATAHDTFWDFISLTPESMHTVM
                     WIMSDRTLPRSLRMIEGFGVHSFRLINDAGESTFVKFHWRPKLGLQSTIWDETVKISG
                     ADPDFHRRDMFEAIAAGNFPEWEFAVQLFTQEEADRFPFDHLDATKLIPEELVPLKVI
                     GRMVLDRWPNNFFAETEQVAYCPANIVPGIDFSNDPLLQGRLFSYIDTQLSRLGSPNF
                     NQIPINAPKCPFSNQQRDGHMQMEQPSGRVAYEPNSLSENSPRETPSNGFHSAALTET
                     GEKGRIRAESFADHYSQARQFYLSQTVYEQAHIASAFVFELSKVEHRHVCEAIVGHLR
                     NIDEDLAKRVAVGLGFDKMPDAPVAAAPVQEMEPSPALQIIGKMKDTLMGRAIGILIA
                     DGSDGAVIKKIKKAATDAGATVKIIAPKVGGAKLADGS"
     gene            complement(1953..2345)
                     /locus_tag="VFC15_05310"
     CDS             complement(1953..2345)
                     /locus_tag="VFC15_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09611.1"
                     /translation="MKGYVQNIESIAVKNEDFRRVLYTAKNLQLVVMALKPKEEIGME
                     AHNSDQFFRVEEGTGEAIINGVRTVISKGFAVLIPGGANHNIINTGNVPLKLYTLYAP
                     PNHRDGVVHHTRAEAEADNEHFDGKTTE"
     gene            complement(2498..>3158)
                     /locus_tag="VFC15_05315"
     CDS             complement(2498..>3158)
                     /locus_tag="VFC15_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09612.1"
                     /translation="RKIDAVLLGDAIDIICNPQLYNIFAPGLAFAFPEGRDEARTFMR
                     RLLDPRNRLAHSNPISLHQAEQVVCYSADIIESLKQHYGAIGMQSEYNVPTILKFLDS
                     FGNRCFRNQMSPGATGGVIMNLSKQPTSVLWPGDVLTVEIEVDPAFDPTDYTISWTCL
                     VNPVESISNGPKATINITTKHVDEHMMIQCKISSHNEWHRMGVGGDDSLSVFYKVRPP
                     R"
BASE COUNT          673 a          865 c          863 g          757 t
ORIGIN      
        1 gagccatcgg caagcttcgc gccgcccact ttgggggcaa tgatcttcac ggtagcaccc
       61 gcatcggtcg cggccttttt aatcttcttg atgacagcgc cgtctgaacc atccgcgatc
      121 aggatgccaa tcgcgcgccc catgagcgta tctttcatct tgccgatgat ctgcaatgcg
      181 ggcgaaggct ccatctcctg cacgggcgct gcggccactg gcgcatcagg cattttgtcg
      241 aacccaagac ctacggcgac ccgtttggca agatcttcat cgatattgcg caggtgaccg
      301 acgatcgcct cgcacacgtg acgatgttcg accttcgaca gctcaaacac gaacgccgag
      361 gcgatgtgcg cctgctcata cacggtttgg ctgagataga actgccgcgc ctggctgtag
      421 tggtcggcga agctctcggc acggatgcgg cctttctcgc cagtttcagt tagcgcagcg
      481 ctatgaaagc cgttggacgg cgtttcgcgt ggcgagtttt cagacaagga gttgggctca
      541 tacgcaacac ggcccgacgg ttgctccatc tgcatgtgtc cgtcgcgttg ttggttggag
      601 aacgggcact tgggcgcgtt gatcgggatc tggttaaaat tgggcgaacc caggcgcgaa
      661 agctgcgtgt cgatatagga gaataaacgg ccctgcagca ggggatcatt cgagaaatcg
      721 atgcctggca cgatattggc ggggcagtaa gccacctgtt cggtttcagc gaagaaattg
      781 ttcggccagc gatcgagcac catgcggccg atcactttca aaggcaccag ttcctcggga
      841 atcagcttgg tcgcgtccag atgatcgaac gggaatcggt ccgcttcctc ctgcgtgaaa
      901 agctgaaccg cgaattccca ctcggggaaa ttacccgcgg cgatggcctc gaacatatcg
      961 cggcggtgga agtctggatc agcgccggaa atcttgacgg tctcatccca aatggtggac
     1021 tgcaagccaa gcttgggacg ccagtggaat ttgacgaagg tcgattcgcc ggcatcgtta
     1081 atcagccgga aactatgcac accaaagcct tcgatcatcc gaagcgaacg gggcagggtg
     1141 cgatctgaca tgatccacat caccgtatgc atggattcag gcgtgagcga aatgaaatcc
     1201 cagaacgtgt cgtgcgcggt cgccgattgt ggaaagccgc ggtcgggttc catctttacg
     1261 gcatggatga ggtccgggaa tttgatggca tcctggatga agaaaactgg gatgttgata
     1321 ccgaccaagt cccagttgcc ttccttggtg taaaacttga cggcaaaccc gcgtatgtca
     1381 cgcggggtat cgaccgaacc cgcgccgcct gcgacggtcg atatgcgagt aaatacgggc
     1441 gtcttctcac ctacctcggt gagtatcctg gcggtggtgt attgcttcaa cgaggcggtc
     1501 agctcgaaga atccatgcac gcccgtcgct cgcgcgtgaa cgatacgctc gggaatacgc
     1561 tcgtgatcga aatgcgtgat tttttcgcga aggatgaaat cctccagaag cgtagggccg
     1621 tgcggattgg ctctgagcga gttttgatta tcggaaagcg cgacgccctg gttcgtggtg
     1681 agcgcgggat gctctccacc agcgatctgg tgcagctcgc caccggcagc cacttgtgag
     1741 tctccattgc gagccgagcc cttttcctgt ttgcgttttc cagaattact cgtcgtccct
     1801 tttttgctca tgatccatct ccttagtgtg taacgcaaac ctgctggcta ttgtcgtgtg
     1861 ctgtcaattt cgacgggccg cttctcatcc cggtccggag gagctgccgg cccaagtcct
     1921 tgctctcccc aagacggcgt tcagtcagat ctctattccg tcgttttacc gtcgaagtgt
     1981 tcgttgtctg cctccgcttc ggcgcgggta tggtggacaa caccgtcccg atgatttggc
     2041 ggcgcataga gcgtgtagag cttcagtgga acattgccgg tattgatgat gttgtggttc
     2101 gccccaccag ggatgagcac ggcgaagccc ttgctgatca ccgtgcgaac gccattgata
     2161 atggcctcgc ccgtcccttc ctccacgcga aagaactgat cggaattgtg cgcttccatc
     2221 ccgatctcct ccttgggctt caacgccatg acaacgagct gcaagttctt ggccgtgtaa
     2281 agcacccggc gaaaatcttc attcttgacg gcaatacttt caatgttctg tacatagccc
     2341 ttcatagtag ctcctccagt tgacgatcct aacatcgtca ttagttagat tgccccaaag
     2401 aagaggcacc tcttttacga cagccaacat ctgaattcac cggcttgcgc atgtccgata
     2461 gaatgatagg ttattcacgc cgccgccttg gtcgtaccta ccgcggaggt cggactttgt
     2521 agaaaaccga caaggagtca tcccctccca cgcccattct gtgccactca ttatggcttg
     2581 aaatcttgca ctgaatcatc atgtgttcat caacgtgctt tgtcgtgata ttgatagttg
     2641 ccttaggccc attggagatc gattctacag gattcaccaa gcatgtccaa cttatcgtgt
     2701 aatcggttgg gtcgaatgcc ggatcaactt caatctctac tgtcaggacg tcgccgggcc
     2761 aaaggacgct cgttggttgc ttactgagat tcatgattac cccacctgtg gcgccaggcg
     2821 acatctgatt tctaaaacac cgattcccga aagagtccaa gaacttgagg atggtcggaa
     2881 cgttgtattc gctttgcatt cctatcgctc catagtgctg cttcaaagac tcgataatgt
     2941 cggccgagta gcagactact tgctctgcct ggtggaggct gatcggattt gaatgagcca
     3001 atctgttccg tgggtctaaa agtcgccgca taaacgtcct tgcttcatct cgcccttcgg
     3061 ggaaagcaaa tgctaaacct ggggcaaaaa tgttataaag ctgagggttg caaatgatgt
     3121 cgatggcatc accaagtaac actgcgtcta tcttgcga
//
LOCUS       DASRKK010000246         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_897222, whole genome shotgun sequence.
ACCESSION   DASRKK010000246 DASRKK010000000
VERSION     DASRKK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897222"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFC15_05320"
     CDS             complement(<1..808)
                     /locus_tag="VFC15_05320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM09613.1"
                     /translation="MDTLIGKSFLHYKIVRQLGAGGMGVVYEAEDTRLARPVALKFLS
                     KDIEQDSYALQRFQQEARAASALNHPNICTIHAIEECDGQHFIAMELLEGQSLNEKIN
                     GHSLPMDKIVDIGIQITDALDVAHSKGIVHRDLKPANIFLTTRGQAKILDFGLAKLIY
                     DRRTVVETVAGDAVTSAPAHLTLPGTAVGTIAYMSPEQARGEELDGRSDLFSLGAIFY
                     EMATGRIPFEGNTSAVVFQGILDRNPRPPMEMNPAVPFKLEEIISKALEKD"
     gene            1350..1838
                     /locus_tag="VFC15_05325"
     CDS             1350..1838
                     /locus_tag="VFC15_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZM09614.1"
                     /translation="MHLTKVKAFLDGHEVKYVVMTHSKAYTAQGIAAVAHISGKFLAK
                     TVIVKLDGALAMAVLPASYQVDLIALKKEVGVKDAELASEREFKQHFPDCETGAMPPF
                     GNLYGIPVYVDETLTEDDEIAFNAGSHHELIRMAYTDFDRLVRPEVMNFSSARTAQLV
                     MV"
     gene            complement(2015..3649)
                     /locus_tag="VFC15_05330"
     CDS             complement(2015..3649)
                     /locus_tag="VFC15_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZM09615.1"
                     /translation="MASSSRRSAVLILSIILACGCVGVVFGQRLTSSAPSGDGDIRDS
                     LRSFTQVYEVVQQNYAEPVSPDKAIYNGAIPGMLRVLDPHSNFFDPKAYAALREEQRG
                     KYYGVGMQVGPRNNKVIVIAPFTGAPAYRAGIRPGDIIIAVDGKPTDNMTTSDVAELL
                     KGPKGTAVKITMLREGSDKPLDFTVVRDEIPRYSVDVHFMVRPGIGYIHVSGFQETTE
                     KEVREALDELGDIHGLILDLRQNPGGLLSEGVGVADQFLKKGQVIVSHHGRASAEKVY
                     KAAHGNGGKDYPLVVLVNRGTASAAEIVAGAIQDHDRGLVAGETTFGKGLVQTVYPLS
                     ENTGLALTTAKYYTPSGRLIQRDYSSISLYDYYYNDRNNDANNANHEVKMTDSGRTVY
                     GGGGITPDVKIPPAKTDKFQDALLEKYTFFNFAKHYVINHKVDQQFAVDDGVMQDFRK
                     FLDEQKIPFTEADLAENGDWIKSSIKAELFINEFGQQEGMKAHAESDPAVAKALELLP
                     QAKQLADNAKRIIAQRNNARLTAQQSDTPEPQSVKR"
     gene            4197..4466
                     /locus_tag="VFC15_05335"
     CDS             4197..4466
                     /locus_tag="VFC15_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZM09616.1"
                     /translation="MIKLDLINEVVTKTGVTKTKAETAVETVFESMKKALARGDRIEL
                     RGFGVFTVRPRKTGIGRNPRTGAEVNIPPGKAVRFKPGKELQSIE"
     gene            4593..5588
                     /locus_tag="VFC15_05340"
     CDS             4593..5588
                     /locus_tag="VFC15_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZM09617.1"
                     /translation="MSDPTPPISYTFEVPARPPVYIVAPRPRRPYWLHILLLLVTLFT
                     TLTVGARLQYNFLHNLPAFSDDSVVLPLFHLRWLLQHPANLVLGIPFSMTLMGILLAH
                     EFGHFVVAKRNGVYATLPYFIPAPTLIGTFGAVIRIKSPIRSRQALFDIGIAGPIAGF
                     LVALPVLFWGLALSKPMPPTAADSALSLGYPLVFGLVHRALAPFTPHAVSLGSLYLHP
                     VGIAAWVGMFATALNLLPGGQLDGGHIIYAVAPRAHKWVTRACILALIPLGIFYWPGW
                     MVWALILGFTGLRHPNVPQWPPLDTNRRWLVLLAVLLLALTFALAPIRGGSLFGS"
BASE COUNT         1080 a         1706 c         1692 g         1287 t
ORIGIN      
        1 catctttctc cagcgccttg gagataatct cctccaactt gaacggcacg gctggattca
       61 tttccatagg cggtctcggg ttgcggtcga gaatgccctg gaaaacaact gcggaagtgt
      121 tgccttcgaa cgggatcctc ccggtagcca tttcgtagaa gatggcgccc aaggagaaca
      181 ggtcgctgcg tccgtcgagc tcttccccgc gggcctgctc tggagacatg taggcgatgg
      241 tgcccaccgc ggtgccgggc agcgtcagat gcgccggcgc gctcgtcacc gcatcgccag
      301 ccacggtctc caccacggtg cgccggtcat agatgagctt ggccagtccg aagtccagga
      361 tcttcgcctg tccccgcgta gtgaggaaga tgttggcggg cttcaaatct cgatgcacaa
      421 tgcctttgct gtgggccacg tcgagggcgt cggtaatctg aatgccgatg tcgacgatct
      481 tgtccatcgg cagcgaatga ccgttgatct tctcgttcag actctggcct tccagcagct
      541 ccatggcgat gaagtgctgc ccatcgcatt cctcgatggc atggatggtg caaatgttgg
      601 gatggttcaa ggccgaggcg gcacgcgcct cctgttggaa acgctgcagg gcgtagctat
      661 cctgctcgat gtccttggag agaaatttca gcgctaccgg gcgggcgagc cgggtgtctt
      721 ccgcctcgta caccacgccc atgccgccgg cgcccagctg ccgcacgatc ttgtaatgca
      781 agaagctttt gccgatcaga gtatccaagg ctagaatctc cggataattc gactgtattg
      841 tgcggtgcga gcagccgtca aggatgggca gccgctgggc tgtgcccggc aagccagtgt
      901 cattttgagc tgtcatcagg aacggccgaa ccggggtccc caacgatcgc cgtttttgcg
      961 cttgttgggg caaagtttag gccgtgaggg atctgctgct tctccggcgg ttgttccagc
     1021 gctaaacaaa agcagattcc tcgctgcgct cgtaatgaca actccgctgc ttatgacgcg
     1081 attctgtact gtcacaaggg tagtgtgacg gctgtgcttg cgcgctgcgc cggccggact
     1141 aaactggggc tacacgaggg tcaaccgacc gcagggaggt agcccgaatg gtcaaagctc
     1201 ttctcgagca agcgcgagca acctcgcgga aatccagcaa acaagcaaag cggatgaaag
     1261 aggccgacga cgaggccttg cactaagttg gtcgcatcaa ccatatcttt ccgggccgtg
     1321 ggcccgcgga atgtcgcgag gagttcgata tgcatctcac caaagtcaaa gccttcctcg
     1381 acggccatga agtgaagtac gttgtcatga ctcactccaa ggcttatacc gcccagggta
     1441 ttgccgcggt cgcgcatatc tcgggcaagt ttctggccaa gaccgtaatc gtgaaactcg
     1501 atggcgccct cgccatggcg gtgctgccgg cgtcctacca ggtggacctg attgcgctga
     1561 agaaagaggt cggagttaag gatgcggaac tggccagcga acgcgagttc aaacagcatt
     1621 ttcccgactg cgagaccggc gccatgcccc cgttcggcaa cctatacggc atcccggtgt
     1681 acgtcgacga gaccctgacg gaagacgatg agatcgcctt caatgcgggc tcgcatcatg
     1741 agttgatcag gatggcgtac accgatttcg ataggttggt gcggcctgag gtgatgaact
     1801 tttcgtccgc acggacagcc cagctggtga tggtttaaac ggccttgatc gagcggagcg
     1861 ggacaaatcc aagcctcgcc cgccagtgtt ggcgatgctg ttccgctccg ccgatcagct
     1921 aacttttcct cggaaggatt ggagtcggtc gcagcagtca cagcaaatga aaagcccgct
     1981 cccgcgggct ttgttgtcaa tctccggtgc gcggttaacg ttttacggat tgcggttccg
     2041 gcgtatcact ctgctgcgcc gtcagcctgg cgttattgcg ctgcgcgata atccgcttgg
     2101 cattgtccgc tagctgcttg gcctggggaa gcagttccag cgccttggcc acagccgggt
     2161 cgctttcggc atgtgccttc atgccttcct gctgaccaaa ttcgttaatg aacagttcgg
     2221 ccttgatact ggatttgatc caatcgccgt tctcggccaa atccgcctcg gtgaagggga
     2281 tcttctgttc atccaggaac ttgcggaagt cctgcatcac cccatcgtca actgcaaatt
     2341 gctggtcgac tttgtggttg atgacgtagt gcttggcgaa gttgaaaaac gtatatttct
     2401 ccagcagcgc gtcctggaac ttgtcggttt tcgccggcgg gatcttcacg tcgggagtga
     2461 taccgccgcc gccgtaaacg gtgcggccgc tgtcggtcat cttgacttcg tggttggcgt
     2521 tattggcatc gttattgcga tcgttatagt agtagtcgta aagcgagatg ctgctgtaat
     2581 cgcgctggat caggcggccg ctgggcgtgt aatacttcgc tgtggtcagc gccagtccgg
     2641 tattctccga cagcggatat acggtctgca ccaacccctt gccgaaggtg gtctcaccgg
     2701 caaccaatcc ccggtcgtgg tcttgaatcg cgcccgcgac gatctccgcc gccgaagccg
     2761 ttccgcggtt caccagtacg accaatggat aatccttgcc tccgttgccg tgggcagctt
     2821 tgtaaacctt ctctgctgaa gctcgcccgt gatgggaaac gatgacctgt cccttcttca
     2881 ggaactggtc cgccacaccc acgccttcgc tcaacagtcc gccgggattc tgacggaggt
     2941 cgaggatgag cccgtgaatg tcgccgagtt catccagcgc ttcgcgcact tctttttccg
     3001 tggtttcctg aaatcccgag acgtggatgt agccgatacc cggacggacc ataaaatgca
     3061 cgtcaacgct gtagcggggg atctcatcgc gcacaaccgt aaagtcgagc ggcttgtcgg
     3121 agccttcgcg cagcatcgta atcttgaccg cggtgccctt gggccctttc agcaactcgg
     3181 caacatcgct ggttgtcatg ttgtcggtag gcttgccgtc cactgcgata atgatatcgc
     3241 cgggcctgat gccggcccga tacgccggcg cgccggtgaa gggcgcgatc acgatgactt
     3301 tattgttgcg gggaccgacc tgcatgccca cgccgtagta cttgccgcgc tgctcctcgc
     3361 gcaaagccgc ataggctttg ggatcgaaaa agttggagtg cggatccagc acgcgcaaca
     3421 tgcccggaat cgccccgttg tagatcgcct tatcggggct gaccggttcg gcatagttct
     3481 gctgcacgac ttcgtaaacc tgggtaaagc tgcgcaggct gtcgcgaatg tcgccgtcgc
     3541 cggaaggagc ggacgaggtg aggcgctggc caaaaaccac gcccacacaa ccgcaagcca
     3601 gaatgatgct gaggataaga acagcggagc gacgcgaact acttgccatg tttgttcctt
     3661 cggctggccg gggatggacc ttcaagggat gcgcaccgga gcgtgacttt aaggcagtat
     3721 accatgcact ctagctcacc ccgagccccc agatcagccg ctagtttgga tgctggttcc
     3781 ccaagaaggg tgcgaaatcc gtttcatccc caccaccgta acagacgaca gagccgggag
     3841 atggtgagca ggcaggaact gggcagtcag cagtagcacg tggcatttgc cagttaacaa
     3901 caacgcttgt atctcgcata tccccaccgt ggcgtcaggc cagatttcag gatgcgacag
     3961 tttcgcagaa cctgcgaaca gcaggtctcc tccactcgaa cgaaccacgc tgtttactat
     4021 ccactatccc ctaaccactg ttgacaatag ggccgggctt cactaggatt cgcgcatcgg
     4081 cttgtaactc gaagtcatcc taagacttac ggaaacggtg cgagtcgttt acaatcgcat
     4141 gagttgccgc agtctgggct gttctgtccc tgcgagcgga tatagcggag ccacttgtga
     4201 tcaaattgga tctgatcaac gaagtcgtca ccaagaccgg tgtcaccaaa accaaggccg
     4261 aaaccgcggt ggaaaccgta tttgagagca tgaagaaggc gctggcgcgc ggcgatcgca
     4321 tcgagttgcg cggcttcggc gtcttcaccg tgcggccgcg caagaccggc attggacgta
     4381 acccgcgcac cggcgccgag gtgaacattc cgcccggcaa agcggttcgc ttcaaacctg
     4441 gcaaggaact gcaatcgatc gagtaaccac gccggccggg ttctcggcga ggccgaatgc
     4501 cgcggcgcgg agcgtgtgtt cctgccgctt cgaccggagg tgcgcgccgg ttactcgttt
     4561 tccgcggcgt tcgtctatca tccaagtaca cgatgtctga tcccacgcct ccgatctcct
     4621 acacgttcga agttcccgcc cgtcctccgg tctatatcgt ggctcctcgt ccgcgtcgcc
     4681 cgtactggct gcacattttg ttgctgctgg tcaccctgtt tacaacgctg acggttggag
     4741 cgcgcctgca atacaacttc ctgcacaacc tgccggcgtt tagcgatgat tccgtggtgc
     4801 tgccgctgtt tcacttgagg tggctccttc agcacccggc aaatctggtg ctgggcattc
     4861 ccttctcgat gacgctgatg ggtattctgc tggcgcatga gttcggccat ttcgtcgtgg
     4921 ccaagagaaa tggggtttac gcgaccctgc cctatttcat tccggcgccg acgttgatcg
     4981 ggacgtttgg cgccgtcatt cgcattaaat ctccgattcg ctcgcggcag gcattgttcg
     5041 acatcggcat tgccggaccg atcgcagggt ttctcgtggc gcttcccgtg ctgttctggg
     5101 gcctcgcgct ttccaagccc atgccgccca cggcggccga ctcggcgcta agcctcggct
     5161 atcctctcgt cttcggtctc gtgcatcgtg ctttggcgcc gttcacgccg cacgcggttt
     5221 cgctgggcag cttgtatctg cacccggtcg gaattgcggc ctgggtcggg atgttcgcta
     5281 ccgcgctgaa ccttttgccc ggtggacaac tcgacggtgg ccatatcatc tacgccgtgg
     5341 cgccgcgggc ccacaagtgg gtgacgcggg cctgtattct cgcgctcata ccgctgggca
     5401 tcttttattg gccgggatgg atggtgtggg ccttgatcct gggcttcacg ggcctgcggc
     5461 accccaacgt tccgcagtgg ccaccgctcg acacgaatcg ccggtggctg gtgctgctgg
     5521 cggtgcttct gctggccctg acttttgcgc tggcgccgat tcgcggcggc tcgctgtttg
     5581 gaagctagag caacgcctaa acaggctgct ggaaaagttg gaaaagcata tctctcgtgg
     5641 gctgaacttt accccctgca atcccccgaa acttacgtgt tggtcctttt gtggcagtcc
     5701 gcgcatttca ccggcgctgc cttaccctga cggttctgtg tcttatggca atcaatgcag
     5761 agtcc
//
LOCUS       DASRKK010000247         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_604117, whole genome shotgun sequence.
ACCESSION   DASRKK010000247 DASRKK010000000
VERSION     DASRKK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604117"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /locus_tag="VFC15_05345"
     CDS             <1..2249
                     /locus_tag="VFC15_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM09618.1"
                     /translation="SLQGACRMCLVEVEKMPKLQTACTTPVAEGMVVTTESDKIKQAR
                     KAMLELVLANHPLDCPVCDAGGECELQDMTFKYGAAESKFMDMKNHRDEQQWSPVVYF
                     DRPRCILCYRCIRVCGEGMDVWALGIQNRGSGSIIAPNKEDHLECEECGMCIDICPVG
                     ALTSGAYRYKTRPWEMKHVGTVCTHCGDGCKTTLGVRRADTGMQIVRGDNRDKSGING
                     DFLCIKGRYAFDFANSGDRIQQPLIKHEGKFVTATWEQALERIASRFKEVLASDGPNA
                     VGVIGSNRTSNEENYLLQKFARLVLGTNNIDHHRTADFPAFASALAGKANATASMRDV
                     FDAPAALLIGNNPTDQHPLLAWQIRTNVRLHRAKLYVVNSYPIKLRRQAAAFAQIAPG
                     SEGSLVQFLAGNDAAADAVIGPQLTRETLSQMREALKNEKDLVVIFGSELRGNDVTAL
                     VNFGLAQGAKFICLGDYANSRGAADMGLYPDMLPGYASVSSTTSFHEDWVDEIPKSPG
                     LNLLQMMDAAKDGKLKALYVVGSNPITRYNVDPFALSKAFVVVQDMFLTETAGIADVV
                     LPIANAYEKGGTFTNTCGDVQMLKKAGEFAGVRNDFECIVRIADRMGYDVHKLVPFGG
                     GGTRADMGQSRGAQSGEADRHAVWLTERNLEPKMSPYDPTAMLDEIQRLVPGYDISRL
                     GLLSGHDEHTQLVQITGATGSGHPELVLPSNDTLFTSGTLGRYSPTLNTVMERYGTTA
                     ADKEIAAD"
BASE COUNT          541 a          791 c          833 g          504 t
ORIGIN      
        1 tgtcgctgca aggcgcctgc cgcatgtgcc tggtggaagt cgagaagatg cccaagctac
       61 agaccgcgtg cacgacgccg gtggccgagg gcatggtggt caccaccgag agcgacaaga
      121 tcaagcaggc gcgcaaggcc atgctcgagc tggtactcgc caatcatccg ctcgactgcc
      181 cggtgtgcga cgccggcggc gaatgcgaac tgcaggacat gaccttcaag tatggcgcgg
      241 ctgaatcgaa gttcatggac atgaagaacc accgcgacga gcagcaatgg tcgccggtgg
      301 tgtacttcga ccgcccgcgt tgcattttgt gctatcgctg cattcgggtt tgcggcgagg
      361 gcatggatgt ctgggcgctg gggattcaga accgcggttc gggctcaatt atcgcgccca
      421 ataaagaaga tcatttggag tgcgaagagt gcggcatgtg catcgacatc tgcccggtcg
      481 gcgcgctcac ctcgggcgct tatcggtaca agacgcgtcc ctgggagatg aagcacgtgg
      541 gcaccgtctg tacccattgc ggcgacggtt gcaaaaccac gctgggcgtt cgccgcgccg
      601 acaccggcat gcagatcgtg cgcggcgaca accgcgataa gagcggcatc aacggtgact
      661 tcctttgtat caaggggcgg tacgcgttcg acttcgccaa cagcggcgac cgcatccagc
      721 agccgctcat caagcacgaa ggcaagttcg ttactgcgac ctgggagcaa gcgctcgaac
      781 gcatcgcttc ccggttcaag gaagtgttgg ccagcgacgg ccccaacgcc gtcggcgtga
      841 tcggctcgaa ccgcaccagc aacgaagaaa attacctgct gcaaaagttc gcgcgtctgg
      901 tgctgggcac caataacatt gaccaccatc gcaccgccga cttccctgca ttcgcttcgg
      961 cgctggctgg caaggccaat gccaccgctt ccatgcgtga cgttttcgac gcgcccgcgg
     1021 ccctgctgat cggcaataat cctactgacc agcatccact gctggcgtgg cagattcgca
     1081 ccaacgtccg cctgcatcgc gccaagctct atgtggtgaa ttcgtatccc atcaagctgc
     1141 gccgccaggc ggctgctttc gcgcagatcg cgcccggctc ggagggcagc ctggtgcagt
     1201 tcctcgccgg aaacgatgct gccgccgacg ccgtcatcgg tccgcaactc acgcgtgaga
     1261 ccctgtcgca gatgcgggag gcgctcaaga acgagaaaga cctggttgtt attttcggat
     1321 cggagctgcg cggcaacgac gtcacggcgc tggtgaactt cggcctggcg cagggtgcaa
     1381 agttcatctg cctgggcgat tacgccaact cgcgcggcgc agccgacatg ggtctttatc
     1441 ccgacatgct gccgggctat gcttccgtct ccagcacgac cagcttccac gaagattggg
     1501 tcgacgaaat tcccaagtcg cccggcttga acctgctgca aatgatggac gcggccaagg
     1561 acggcaagct gaaggcgctg tatgtggtcg gctcgaaccc gatcacacgc tacaacgtcg
     1621 atcctttcgc cctgtcgaag gcgttcgtcg tggtgcagga catgttcctc accgagacag
     1681 ctggcatcgc tgacgtggtc ttgcccatcg cgaatgccta cgagaagggt gggactttta
     1741 ccaacacttg cggcgacgtg cagatgctga agaaagccgg cgaattcgcg ggcgtgcgca
     1801 acgacttcga gtgcatcgtc cgcatcgccg atcgcatggg atacgacgtg cataagctgg
     1861 tgccgttcgg cgggggaggc acgcgcgccg acatgggcca gagccggggc gcgcaatcgg
     1921 gtgaagcaga ccggcatgcg gtgtggctca ccgagcgcaa tctggagccc aagatgagcc
     1981 cctacgatcc caccgcgatg ctggacgaaa ttcagcggct ggtgccgggg tacgacatct
     2041 cgcggctcgg tctgttgagc ggtcacgacg aacacacgca actggtgcag attactggcg
     2101 ccactggttc gggacatccg gaactcgtgc tgccctccaa cgacacgctg ttcacctcgg
     2161 gcacgctggg ccggtattcg cccaccttga acaccgtgat ggagcgctac ggtacgacgg
     2221 cggccgataa agagatcgcg gcggattaga ggaagtggct agtgattagt ggttagtttc
     2281 aaagtttcga gtttcgagtt acaagtttcg agtaggcggc aactgattct gtaaccagag
     2341 caggccttca gagattgcgt cgcgatcttg gtatcggttt gcccaatgtt ggcgccccgc
     2401 tcgggatgac agcgatgttt tgctaagggc taaatgctaa gggccaagag ctaagagccc
     2461 cacaacgaac gtcggctgct gctagccaac agattactga cggctgaaag ctgagtgctg
     2521 atagctacat gggcattgga acgtacatcc tggtttcgct ggtcaagatc gcgatcgcgc
     2581 tccttgtcct gctgacggcc gtggcctaca ccacgttgct ggagcgcaaa gtggttgccc
     2641 acatacagaa ccggtgggga ccgtcgcgg
//
LOCUS       DASRKK010000248         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_18300, whole genome shotgun sequence.
ACCESSION   DASRKK010000248 DASRKK010000000
VERSION     DASRKK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18300"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFC15_05350"
     CDS             <1..181
                     /locus_tag="VFC15_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09619.1"
                     /translation="ALLVESGEPSMDLLEDAVATETMSPQIAGPCMAEILRCGHARET
                     NEKAPKQHLGDVWLC"
     gene            387..1349
                     /locus_tag="VFC15_05355"
     CDS             387..1349
                     /locus_tag="VFC15_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM09620.1"
                     /translation="MRQRTLAMTTGFERYSKKTRRAAFLEEMEQVVPWSALSLLVGPH
                     YPKAGNGRRPVGIDRMLRIYFLQQWFNLSDPAVEEALYDSPLMRQFAQIDLGNEPVPD
                     ETTVCKFRHLLEEHNLGGAMLETVNLHLESRGIKITTGTIVDATIIHAPSSTKNREQK
                     RDPEMHQTKKGNEWYFGMKAHVGVDSKRKIIHSVVVTAANIADSAVLPDLLHGDETRV
                     WGDQAYRGQREAIHEVAPRAQDLTNKRYRYKDRVDEVEREKNRTKSRTRSRVEHVFAV
                     IKLKFGYTKVRYRGIKKNANQVFTLCALSNLFLLRGRLLVTQGA"
     gene            complement(1805..2877)
                     /locus_tag="VFC15_05360"
                     /pseudo
     CDS             complement(1805..2877)
                     /locus_tag="VFC15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611084.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          675 a          910 c          860 g          686 t
ORIGIN      
        1 ggcccttctg gttgagagcg gcgaaccctc gatggattta ttggaggacg ctgtcgccac
       61 ggaaacaatg agcccgcaga tagccggccc gtgcatggct gagattcttc gctgcgggca
      121 tgcccgagaa accaatgaaa aagcaccgaa gcagcatttg ggcgatgtat ggctttgttg
      181 acttgtcttg gaaagtagtg tgggtaacct gtcgtaaaga tcgttccgcg gcggcggact
      241 tcgacttagg gtgcctagaa agctcgaccg ccgtgatgag cgacaggaga agcacccgga
      301 gttcgacaga gttagggaaa ctctgattaa gttctctgct ttccacagtg tcattgtgta
      361 gattgagtcg agagactctg aagacaatga gacagcggac cctagcgatg acaactggat
      421 ttgagcggta cagcaagaag acccgtcgtg cagcatttct ggaagagatg gagcaggtag
      481 ttccatggag cgcgttgtct ttgttggtgg gaccgcacta ccccaaagct ggcaatgggc
      541 gaaggccggt gggcatcgat cgcatgctgc gtatctactt tctgcagcag tggttcaacc
      601 tctctgaccc tgcggtcgaa gaggcactgt acgactcgcc gctgatgcgc cagttcgcgc
      661 agatcgatct tggcaacgag ccggtgccgg acgagacgac ggtctgcaag tttcgtcatc
      721 ttctggaaga gcacaacctc ggtggcgcga tgctggagac agtgaacctt catctggaaa
      781 gtcgtggcat caagatcacc actggcacca ttgtcgatgc gacgatcatt cacgcgccca
      841 gttcgacaaa gaaccgcgaa cagaaacgcg accctgagat gcaccagacg aaaaaaggca
      901 acgaatggta tttcgggatg aaagcgcacg tgggcgtcga tagcaagagg aagatcattc
      961 attcggtggt agtgacggca gccaacatcg cggattcagc ggttctgccg gatctgttgc
     1021 atggtgacga aaccagggtc tggggcgatc aggcctatcg cggacaacgc gaagcaatcc
     1081 acgaagtcgc cccacgagcg caggacttga ccaacaaacg ctatcgttac aaggaccgcg
     1141 tggacgaagt agaacgcgag aagaatcgaa cgaagtcacg cactcgctcc cgagtcgagc
     1201 atgtgtttgc ggtgatcaaa ctgaagttcg gctacacgaa agtgagatac cgggggatca
     1261 agaagaacgc caatcaggtg ttcacactct gtgcgctgag taatctgttt ctgttgcgcg
     1321 gcagactgct ggtcacccaa ggggcgtagt gtctctggac actctccaga ggtgacggca
     1381 acctccaaga gatcaaaaca cgccgtcaaa cacactgaaa tcccagtcac gccaaccccg
     1441 aaaaccctac cctgccgcaa ttcgctaact tgtgcagacc ttccctagaa atatcgaggg
     1501 agttgcggga taatcgagcg gaagttctat ccttctcgaa tagtagcgga agtgggtaga
     1561 gagcgattag ttgtggtaaa ggctgtgatg tgccccccgc ttttgatcca ctcataatga
     1621 gaggtccggg gttgtgaacc gctcccattc tctgtcacag tcggaattag agattctggg
     1681 ccgttgaagt cctcttagcc tgatttaatg caggttcggc gcactccaga gactcgatca
     1741 agaggctctg gagtgcgccc ccaagcgatg tatttgtttc ttgcttctcc tgcctgtgca
     1801 actccgcgaa ctctgccggt gtccggtcag ccaacgcgct gtgcggacgc tgatgattgt
     1861 aatgctggcg gaagatcgcc agtttgttcc gtgcatcttc cagtgatgcg aaccactcta
     1921 cgttcaagca ttcgtcccgt aatcggccat tgaagctttc gatgaatccg ttctccaccg
     1981 gccggccggg ccggataaaa cacaactgaa cttcattggc catggcccat gcttccagtg
     2041 cacgactgca gaactcgctg ccgttgtcca cggtgatggt ttccggcaag cttccacgtt
     2101 cgtccttcgc gcgctccagc gcctgtgcca ctttcatccc tgtcatcgag cgatccgctt
     2161 ccaacaccac acactcgcgt gtgaactgat cgatcatcgt caagattcgg aacgagcgac
     2221 catccgatag cttgtcgctg acgaaatcca tgctccagcg ttgattccgt cgggctgcca
     2281 cggaactcct cgccagcggc cggcggcgcg ccatcttccg ccgctgtttc gtgcgcacga
     2341 tcaactcttc ctcccgatac agccggtaaa tacgtttcgc attcacatgc caaccttctc
     2401 gccgcagcaa taccgtcagg cgtcgatatc cgtagcgcac atgcgttccc gccagctcgc
     2461 gcaaacggcg ccgcaacact tcgtcgaata ttttccggct gcgataccgc aacgtcccaa
     2521 tcgccagcct taccagcctc gccgcgtgcc gctcgctgat ctgataggcc tcctgcgccc
     2581 aacgcgccag cttgcgccgc acccgaggcc tcacagcttt tttgagacga tctcctgcag
     2641 gatctgacgg tccatgctca ggtccgcgac cagccgtttc agcctgccgt tttcttcgcg
     2701 aagctgccgc agttcacgca gctcgctgac tccgaccccc gcatactgtc gcttccacat
     2761 gtagtaggtc gcctggctga tcccaacctt gcgacaaatt tccgccaccc gcgccccggc
     2821 ctccacctgc cgcaataccg ctacgatctg ctcttccgtg tgtcctctct tgggcatagt
     2881 cctcgtctcc tctccaggac tcctgctcca gcttacctct actttcctct gtgtcagttt
     2941 ttgggttttg ggtcaagccg ccggagctgt gggaaagtgg aaaacgcctt gggcgttttc
     3001 caagcgggcg cagcccgcgt ctttgtgtgc cgtgcatgtt cgacagtttg agggtgcaaa
     3061 tcccttatcc aacctgatgg aggtgaagga ttagcgaagc gcaagggcgt cgtcgcgagg
     3121 cggggtctga a
//
LOCUS       DASRKK010000249         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_604161, whole genome shotgun sequence.
ACCESSION   DASRKK010000249 DASRKK010000000
VERSION     DASRKK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604161"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFC15_05365"
     CDS             complement(<1..590)
                     /locus_tag="VFC15_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09621.1"
                     /translation="MRQIDQLTVHPAVKRLICSEFQFFCRTAARDSDLFKLDSYSFWA
                     SCKIALLKRFPAGQYHWELSGFPRSFFFKLPPRAMPRVAYFIAAHMKGFAPCIEPHMP
                     ARSVLILERESNKAWYRMARSVEADLRINGLVAFSWLYSPDTFKASPHLSFINKPFVE
                     SGGLITTMGEADKNSGFLTGSEHRKKLYESGEFKPT"
     gene            complement(906..1772)
                     /locus_tag="VFC15_05370"
     CDS             complement(906..1772)
                     /locus_tag="VFC15_05370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZM09622.1"
                     /translation="MDKLKQVGCNVLGLTEALSRLRAGNLPPRSVALTFDDGWADFYR
                     EAFPILRKHGFPATVYLTTYYCLFNRPIFHFALAYMFWKRQGNVIQNDAHSWLPGELD
                     LRTPESQAALLRQIDAHARQKNLSGKEKDELASQMAALVGFDYQAFLGERLMQLMNPA
                     EVTEVSNGGIDIQMHTHRHRTPLDRNRFIAEIEENRRIINELAASDGAHFCYPSGAVR
                     PDFVSWLQDAGVQSATTCNHGFSSRKTNLLLLPRLLDQCGISDNEFEAWITGFAAFMP
                     KRKLMSPDIAPE"
     gene            2196..2438
                     /locus_tag="VFC15_05375"
     CDS             2196..2438
                     /locus_tag="VFC15_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZM09623.1"
                     /translation="MVSAIDTLVQDALKQRGLPPQPITVDTLLLGSSLGIDSLDLAAI
                     VVQLTEETGKDPFDDGFIDFRTVGELARLYAAFDSQ"
     gene            2459..3760
                     /locus_tag="VFC15_05380"
     CDS             2459..3760
                     /locus_tag="VFC15_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZM09624.1"
                     /translation="MGSTNRRLQFSGSTVRRDGRLIGSEELASAAASTAAALEPFRGR
                     RVALLGQGADQILIALAAAEIAQCQLLLCRTAELAEDLAREWSVAALLDSSMCIRPTN
                     FADAGPPEFSVLIMSSGTTGVPKLAQHSIDSLLGRIRPPKADQPRQKWLLTYHPSTYG
                     GLQVLLSSLIIGAELITVSNPTVANLCAASLKYQPSHISGTPTFWRAFLLTLGPQAST
                     LQLDQITLGGEIADAHILKALRETYSTASISHIYASTEAGALFSVRDGLPGFPAAWLE
                     TGVDGIELRIRESVLQVRSPRGMKRYVARAAAPVLTEDGWLMTGDLVEQVGDRVYFSG
                     RQDTMLNVGGAKVRPEEVEEVLFSFTEIADARVYGVRNPITGVVLAADIVANPGVDVA
                     GLKTTLPANLRARLESYKVPRIFNFVSCIAVSAAGKKKKLG"
BASE COUNT          847 a         1033 c         1098 g          860 t
ORIGIN      
        1 tgagtgggtt tgaattctcc gctctcgtag agcttttttc gatgctcact cccggtcagg
       61 aagccggaat tcttatccgc ttcacccatg gtagtgataa gaccaccgga ctctacgaaa
      121 ggtttattga taaacgacaa atgcggactg gctttgaagg tgtctgggga atataaccaa
      181 gaaaaagcca caaggccgtt tatgcgtaag tccgcctcga ctgaacgcgc cattcgatac
      241 caagccttat ttgattctct ttccaatatc agcacggaac gcgcgggcat gtgcggctcg
      301 atgcacgggg cgaacccctt catgtgggcg gcgatgaaat aggcgacgcg cggcatggcc
      361 cgcggtggga gcttgaagaa aaaagacctc ggaaagccag acagctccca gtgatattgg
      421 cctgctggaa atcgtttcag cagcgcgatc ttgcaagacg cccagaatga atagctgtca
      481 agtttaaaga gatccgagtc gcgagccgcc gttcgacaga agaattggaa ttcactacag
      541 attagacgct taaccgcggg atggactgtc aattggtcga tctgcctcac ggtgctgagg
      601 gcagtctgca cgagaagata tctctgaatg agggcgccgg actccaattc cgatgtcaac
      661 tctggcccca aggcactagt gattgccaac gatcggctgg caccatttcg tatggatatg
      721 accgaccgaa gcacgtccgc ggaggactgc ctattcgcaa gcactcgacg caatcgttcc
      781 ccaatatcag aaaagctggg gtccacgaga gcctggttga gagactcgcg ggtttcatca
      841 atcttgtcgc gaacaggggc tggcatctaa taacttaagt ctcgctacga cgagggacga
      901 tagcttcatt cgggtgctat gtctggtgac attagctttc gcttcggcat gaatgcagcg
      961 aaaccagtta tccatgcctc gaattcatta tcggatatgc cgcactgatc aaggaggcgt
     1021 ggcaataaga ggagattggt cttgcgcgat gagaagccgt ggttgcaggt agtcgccgac
     1081 tgcacaccgg catcctgtag ccacgacaca aaatccgggc gtaccgcacc actgggataa
     1141 caaaaatgcg ctccgtcgct agcggccagc tcgttgatta tgcgccgatt ttcctctatc
     1201 tctgcgataa atcggttgcg gtccagcgga gtccggtgcc ggtgcgtgtg catttgaata
     1261 tcaattccgc cgtttgacac ttcggttacc tcggccgggt tcatcaactg catcagacgc
     1321 tcaccaagaa atgcctggta atcgaatccc accagggcgg ccatttggga ggcaagttca
     1381 tccttctcct ttccggatag gttcttctgt cgcgcatgag cgtcaatttg cctgagtagg
     1441 gcagcctggc tttccggagt tcgcagatcg agttcgccag gtaaccatga gtgggcatcg
     1501 ttttgaatga cattcccctg tcgcttccag aacatatagg ccagcgcgaa gtggaatatc
     1561 ggacggttaa acaagcagta gtatgttgtg aggtagacgg tcgcaggaaa gccgtgcttg
     1621 cgcagaatcg ggaatgcttc tctgtaaaaa tcggcccatc catcgtcgaa agtcaacgct
     1681 acgcttcgtg gcgggagatt gcctgctcgt aaccggctta acgcctccgt caatccgagc
     1741 acattgcagc caacttgctt cagcttgtcc attcgccttt cgaaagtcga cgggagcatg
     1801 aacatttcgg gatgggagcc atgctcgtcg cccatcgaga tgccatgata gcacaatatc
     1861 agcaagcggt ttcgacggaa tgtgctattc gacaagagcg agaaggttcc catgcccttt
     1921 gttgcgagca atgagtcttt tatggccctt agcacactca tggggtttct cttgagattt
     1981 tgggattccg cgcatctgcg agcaatgata cacggtcact tctgccagac gccacccttg
     2041 gcgagcgtcg ataatcacag cattgttgcg atattgctcc ctacctaccc gcaccgctcg
     2101 tgcgctgcag tcaaactctc ggctgggata tactcatctc gaacaaccct tacaggaaag
     2161 gcatcgccga ccaatcgtgc atgaatgtca ctcaagtggt ctcagcaatc gatacattag
     2221 ttcaggatgc actcaagcaa aggggcttgc cgcctcagcc cataaccgtc gacaccttgc
     2281 ttctggggtc ttcgcttgga attgactcgc tggatctcgc tgcgattgtg gtgcaactga
     2341 cggaagaaac tggaaaagac ccgtttgacg acggatttat cgactttcgc acggttggcg
     2401 aactggcgcg gctgtatgct gccttcgaca gccaatgatt gattgcaagc cggacagatt
     2461 gggttcgact aaccgcagac tgcaattcag tggctcgaca gtacggcgcg atgggcggtt
     2521 gatcggcagc gaggaactgg cgtctgcggc ggcctctacc gcagcagcgc ttgagccgtt
     2581 tcgcggacgc cgggtggctc tgctggggca aggcgcggac cagattctga tcgccctagc
     2641 ggcggctgag attgcacaat gccaattgtt actgtgccgg actgcggaat tggcggagga
     2701 cctggctcgg gaatggagtg tcgcggctct gctcgactcc tcgatgtgca tacggccgac
     2761 taactttgcg gatgccggcc cgccggaatt ttccgtgttg attatgagct ctggaacaac
     2821 cggagtccct aagctcgccc aacattcgat cgattcgtta ctgggtcgca tacgaccacc
     2881 gaaggccgac cagccgcgcc agaaatggct gctcacttat cacccttcga cttacggcgg
     2941 actacaagta ctgctgtctt ccctgatcat tggggcagaa ctaatcacgg tctcgaatcc
     3001 gacggtcgcc aacttgtgtg ccgcctccct gaaatatcag ccctcgcaca tcagcggaac
     3061 gccgactttt tggcgcgctt ttctactgac gctcgggccg caggcgtcta ccctgcagct
     3121 tgaccaaatc acgctggggg gagagattgc ggacgcgcat atcctgaaag cgctgcgcga
     3181 gacctattcc accgcttcga taagccacat ttatgcttcg actgaggcgg gcgctttgtt
     3241 ttcggtgcgg gatggccttc cgggtttccc tgcggcatgg ctggaaacgg gggttgacgg
     3301 aattgagtta cgcattcggg aaagcgtgct gcaggtgagg agtccgcggg gcatgaagcg
     3361 atacgtcgcc cgagccgcag cgccggtgct aacggaggac ggctggctca tgactggcga
     3421 tctggtcgag caggtcggag accgcgttta cttttccggc aggcaggaca cgatgctgaa
     3481 cgtgggcggc gcgaaagttc gtccggaaga agtggaagag gttctgttca gctttacgga
     3541 gatcgccgac gccagagtct acggcgtgcg caatccgatt accggcgtcg tgctggcagc
     3601 ggatatcgtt gcgaacccag gcgtagacgt ggcgggtctg aaaacaaccc tgcccgccaa
     3661 tttacgggcc cggctggagt cgtataaagt cccgcggatc ttcaatttcg tcagctgcat
     3721 tgcggtctcg gcggctggca agaaaaagaa actaggctga tatgacaatt caaaagagcg
     3781 accgaacgat cctggtcacc ggtggaagcc gtggtcttgg gctcgcccta gtgcgcac
//
LOCUS       DASRKK010000250         6829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_995633, whole genome shotgun sequence.
ACCESSION   DASRKK010000250 DASRKK010000000
VERSION     DASRKK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995633"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..986
                     /locus_tag="VFC15_05385"
     CDS             534..986
                     /locus_tag="VFC15_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09625.1"
                     /translation="MTSRRMQFGVLLAVCSCLILPACRKEGAEGGKVGGGQQSAVTIT
                     LSTDNNGNCVQSQGGATVTNATVHYDQSVTWCAVDQSGNKLAFDLQFAQGASPFAQNQ
                     FTATAGNCSSPSGTPTNAMERIYPYQQITINGKQCGVGSDGMHIKPGT"
     gene            complement(990..3587)
                     /locus_tag="VFC15_05390"
     CDS             complement(990..3587)
                     /locus_tag="VFC15_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM09626.1"
                     /translation="MAIQAPSSGRVLGHYRLIEQIGAGGMGVVFRARDEQLQRDVAVK
                     ILPAGLFADDATRKQFRKEALAVGKLNHPNIAMAFDFGEENGVDYLVTEYIPGLNLDE
                     MLVQQALPQKTVLELGIQLASGLEAAHRENVIHRDLKPGNLRVNPDGQLKILDFGLAK
                     LVEPIDEVAETANLNTSLSLSGTVPYMAPELLRAETADARSDIWAAGAVLYEMATGKR
                     AFPDRQPSLLIDAILHYDPVRPCLLNPEVSAALETVILKALDREPDLRYQSARELRAD
                     LSRILAGDELSTETLRRTSFFEVERKARHRKAALILAAVLLAGVAVGYGAKRWWRTPS
                     SNQQRIMAVLPFDTVGQDPATSALGLGLTETLTAKLVQASDTDAIQVVSPRDLRDQGV
                     KTAEDARREFGTDFVLESSLQRSGQTIRVNCFLVDSKTHRQIAARSITVDAGDAFGLQ
                     DRVVSETLDMLPAQIKPERRRQLNAQHDTQPAAYEAYIRGRGYMQEPGPDNIEKAIAE
                     FTKAIQIDPNYALGYAALGNSYWVGFQQYAKPNGWVPNASRNCQKALSVNPELVAGHV
                     CLGRLLTGSGKYDQAVEEFQRAVRAEPGSEDALLGLAEAYSHLGDLAAAESTYKKAVA
                     LRPEYWGVYSWLGTFYFGQNRYADAAAAFLKSTQLAPNNYLGYFNLGGAYGLEGRYSE
                     AIDALKRSIELRPSLDAYGNLGYTYTLMHRYPEAIAALEQALKIDDHDWLNWGNLGDA
                     LYWSPSRRAEAEAKYRKAIAIAASKLEVNAGDVPTLAYLADYSAMVGDRQTAITYIEK
                     ALKLAPSNGEALFRAALVYNHFNQTNQTLTYLTKAVEAGYSRTVVRDTPDFGNLQQHP
                     MFRQLAATP"
     gene            complement(3710..4840)
                     /locus_tag="VFC15_05395"
     CDS             complement(3710..4840)
                     /locus_tag="VFC15_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HZM09627.1"
                     /translation="MTATNFEIPIEVAKAPETFARLVSLDVFRGATIAAMVLVNNPGN
                     SAAYWPLTHAKWNGWTPTDLIFPYFLFIVGVSLVLSFRSRLRRGDSTRSLVWHTVKRS
                     LTIFALGLLLNGLPPFHLATWRIPGVLQRIALAYFAAAMITLYCKTCARMLWIASLLV
                     GYWLLMRYVPVPGFGVPGRDIPFLHPNANLAAYLDRKLMMGHLYEGTRDPEGVLSTLP
                     AIATTLCGGLTGEWLGSNRRKTQKMLGMLALGAAGLIAGEVWSFWFPINKNLWTSSYV
                     LFTAGCALLCLAICYWITDIRQYRGWLTQPFIIFGTNAIAAYVLSELVGVPFRWLDYI
                     FQQPFARAVSPAMASLLYSLTIVGLCFLPIWWMYRRRIFLKV"
     gene            5301..6539
                     /locus_tag="VFC15_05400"
     CDS             5301..6539
                     /locus_tag="VFC15_05400"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZM09628.1"
                     /translation="MKRQKRRRQILWGSIAIVVLIGVTVGISRLKPAAPTVDRSTIWT
                     DSVKRGSMLRQVRGLGTLIPSHEDVRQIPAETEATIIRIVKLPGSLVEADTVLVEMSN
                     PQLEQEALDAQLQVKAAEAEYQSLKVKLASDLMTQKAGAATVNADYAEAQRQAQTDKA
                     LYDLGVISGLAAKASAGKAQELVTRNDIEDQRLQINQKAIASQLAVQQAKVEQMRALA
                     DLKQKQLQRLKVQAGVAGVLVDLPLQVGQHVLPGAELARIVQPNHLMAELKIPETQAR
                     DVQIGEPASVDTHNGVIPGEVSRVDPGVQNGTVTVDVKLTGELPKGARPDLSVDGTID
                     LERLENVLYVGRPAFGQETSTISMFKLDPEGKAATRVQVKTGRASVNLIQIESGLHEG
                     DTVILSDMSRWDNTDRVRLE"
BASE COUNT         1530 a         1916 c         1995 g         1388 t
ORIGIN      
        1 cagcgccgtc aagtatcacg accggattaa tcagctcaag aagcagttga ttatcgatgc
       61 ggtcgcgcaa gccaagggaa actacaccga agctgccaat ttgcttggac ttcatcccaa
      121 ctacctgcac aggctcatca ggaacctgga tatcaagggg gagctgaagg gcgactgaac
      181 gcgggcggca ctcggctgtc ggctgttcgc cattcgctct tcacgattcg ctttgtacga
      241 ttcagttttg gccctcactt tcagctccga gctttgtgct ggatcgttta agaattgtca
      301 tgacgagcgc agcgaggcat ctgcttttct gttgggaccg gcagtaatcc cagcggtgaa
      361 aagcaggtcc ctcgctgcgc tcgtgatgac aaccctatcg aatgctccaa ataggtgtgg
      421 cggcggtatg tccgaaaagt ggtgagaccg ccgctggcac cgcacttgca acagtttcac
      481 ttgacagcaa ctcacagcgt ggtagtgttg cacacaaaag aaaggaacct gccatgactt
      541 cacgccgcat gcagtttgga gtattgctgg ctgtttgctc ttgtctgatt ctgccagcgt
      601 gccgcaagga aggtgcagaa ggaggaaaag tgggaggagg ccagcaaagt gccgtgacta
      661 tcaccctgtc taccgacaac aatggcaatt gtgttcagag ccaaggcggg gcaaccgtta
      721 cgaatgcgac cgtacactat gaccagtcgg ttacctggtg cgccgtcgat caatccggga
      781 acaagcttgc ctttgacctg cagttcgcgc aaggtgcgtc tccgttcgcg caaaaccaat
      841 tcactgccac cgccgggaac tgctcgtcgc cctccggtac ccccacgaat gcgatggaaa
      901 ggatctatcc ttaccaacag atcaccatca acggcaaaca atgcggagtc ggttccgacg
      961 gcatgcatat caaaccgggg acgtaagctt tacggagtgg ctgccagctg acgaaacatc
     1021 gggtgctgtt gcaggttgcc gaaatcggga gtatcacgga caaccgtgcg cgaatagccc
     1081 gcttctacgg ccttcgtcag ataagtaagc gtctggttag tctggttgaa gtggttatac
     1141 acaagggccg cgcggaatag cgcctcgcca ttcgaaggcg ccagcttcag cgctttttcg
     1201 atgtaggtga tcgccgtctg gcggtctccg accatagctg aataatcagc caagtaggcc
     1261 aaggtgggca catcgcccgc attgacctcc agcttggaag ctgcgattgc aatcgctttg
     1321 cgatacttgg cttctgcctc agcgcgcctg ctcgggctcc agtagagtgc atcgccaaga
     1381 tttccccaat taagccaatc atgatcgtcg atcttgagcg cctgttccag cgctgcgatc
     1441 gcctcggggt agcggtgcat cagggtatag gtgtagccca ggttgccgta cgcgtctaag
     1501 ctgggccgta gttcaatcga gcgcttcaat gcatctatgg cctccgagta acgtccctcc
     1561 aaaccataag ccccccccag gttgaagtag ccaagatagt tgttgggtgc cagttgcgtg
     1621 gattttagga aggctgccgc tgcgtccgcg tagcggttct gtccgaagta gaatgtgcct
     1681 agccaactgt atacacccca gtactcgggc cggagcgcga ctgccttttt gtaggttgac
     1741 tcggcagcag cgaggtctcc caagtggctg tatgcctcgg ccaaaccgag gagcgcatct
     1801 tcgctgcccg gctcggccct cacagcgcgc tggaattctt cgacggcttg gtcatacttg
     1861 ccagaaccgg tcaggagtct tccaaggcaa acatggcctg ctaccagctc cgggttcacc
     1921 gacagtgcct tttggcaatt gcgggaggca ttagggaccc agccgtttgg ttttgcgtac
     1981 tgctgaaatc ctacccaata gctattacct aacgcggcat agccaagcgc atagttggga
     2041 tcgatttgga tcgccttggt gaactccgca attgcctttt ctatattgtc gggacccggt
     2101 tcctgcatgt aaccccgtcc ccgaatgtag gcttcatacg cggcgggctg ggtgtcgtgc
     2161 tgtgcattca gctgacgccg tcgttctggc ttgatttgtg ccggcagcat gtccagcgtc
     2221 tcgctcacca cacggtcttg cagtccaaag gcgtcgccgg catccacagt tatgctgcgg
     2281 gctgcgattt gccggtgcgt cttggaatcc acgagaaagc agttgacgcg gatggtctgg
     2341 ccggagcgct gcaggctgct ctccagaacg aaatcggtgc caaactcgcg gcgggcgtct
     2401 tccgcagtct tgactccctg gtcgcgcaga tcgcgcggcg agaccacctg gatggcatcc
     2461 gtatcgctgg cttggaccaa cttggcggtg agggtttcgg tcaggcccag accgagagcg
     2521 ctggtcgcgg ggtcttggcc tacggtgtcg aagggcaata cggccatgat tcgttgttgg
     2581 ttggaggacg gtgttcgcca ccaccgcttg gcgccgtatc caactgcgac tcccgccagc
     2641 aataccgctg ccaggatcaa tgcggctttt cgatggcggg ccttgcgctc aacctcgaaa
     2701 aagctggtac ggcgaagagt ttccgtggac agctcatcac ctgcgaggat ccggctaagg
     2761 tccgcccgca actcccgcgc cgactggtag cgcagatcgg gctccctatc caatgccttc
     2821 aggatcacgg tttctaacgc agccgaaact tcgggattga gcaggcaggg gcgcacgggg
     2881 tcgtaatgca gaatggcatc gatcagcagc gacggctgac ggtccggaaa tgcgcgctta
     2941 cccgtcgcca tttcatagag cacggcgccg gctgcccaaa tgtcggagcg ggcatcggcg
     3001 gtttcagcgc gcaacagttc gggcgccatg tagggcaccg ttccgctgag cgagagactg
     3061 gtgttgaggt tcgcagtctc ggccacctcg tcgattggtt caaccagttt ggccaggcca
     3121 aaatcgagaa ttttcaactg tccgtcgggg ttgacccgca gatttcccgg cttgaggtcg
     3181 cgatggatga cattctcgcg gtgcgcggct tccagcccgc tcgccagttg tatgcccaat
     3241 tccagcacgg tcttttgcgg aagcgcctgc tggacgagca tctcgtcgag attgagcccg
     3301 gggatgtatt ccgtgaccag ataatcgacg ccattttcct ccccgaagtc gaaggccatg
     3361 gcgatgttgg gatggttcag cttgccaacc gccagcgcct ccttgcggaa ctgcttgcga
     3421 gtggcatcgt cggcgaacaa tccggcgggc aggatcttga ccgcgacatc gcgctgcagt
     3481 tgctcatcac gggcgcgaaa gaccacgccc atgcctccgg cgccaatctg ttcgatcagc
     3541 cgataatgcc ccaatacccg tccgctgctg ggggcctgaa ttgccattga cccggatatt
     3601 agggcagcgc gcaaaggccg tcaaataggg cgctgtgtcc gcaccggttg cgagcgcagt
     3661 cttgttgact tctagcacgg gcttgggaat cgctaaacga ttgcagcggt caaaccttga
     3721 gaaaaattct tcttcgatac atccaccaga tgggcaaaaa acacagtccg actatggtaa
     3781 gcgaatacag cagcgatgcc atggccggag aaactgcacg ggcgaagggc tgctggaaaa
     3841 tatagtccag ccaacggaac ggcactccca ccagctccga taatacgtac gcggcaatgg
     3901 catttgtgcc gaagataatg aagggttgtg tcaaccaacc tcgatactgc cggatgtcgg
     3961 ttatccaata acagatcgcc agacaaagca gcgcacatcc cgcagtgaac agcacatacg
     4021 aactggtcca caggttcttg ttgattggga accagaagct ccaaacttcc ccagcgatca
     4081 gtccggccgc acccagggcg agcattccta acatcttttg cgttttgcgg cgattcgagc
     4141 ccagccactc tcctgtcaaa ccgccgcaca gcgttgtggc aatcgcgggt agtgtgctga
     4201 gcacgccctc gggatcgcgc gtcccttcgt acagatggcc catcatgagc ttgcggtcga
     4261 ggtacgcggc caggttggcg ttcgggtgaa gaaaaggaat atcgcggcca ggcactccga
     4321 atccgggcac cggcacatat cgcatcagga gccaatagcc caccaagagg gatgcaatcc
     4381 atagcatgcg tgcacaggtc ttgcagtaga gcgtgatcat cgcagcggcg aagtaggcga
     4441 gcgcaattcg ttgcagaacg ccggggattc tccaggtcgc cagatggaag gggggaagtc
     4501 cattcaacaa caggccgaga gcgaagatcg tcaggctgcg ctttaccgtg tgccagacca
     4561 gcgatcgagt ggaatctcca cgtctcagtc gcgatcggaa ggaaagcaca agcgagacgc
     4621 cgacaatgaa gaggaagtag ggaaagatca agtcggttgg ggtccaaccg ttccacttcg
     4681 catgcgtaag cggccaatag gcagcactgt ttccggggtt gttgaccagg accatggccg
     4741 cgatggtggc accgcgaaag acatccagcg agaccagccg cgcaaacgtt tctggcgcct
     4801 tcgcgacctc aatcgggatc tcgaaattag ttgctgtcat acgcagacgc gaccgacttt
     4861 gaaatcttga agtgagcaca aggtttggcg tcgcgcagtg gaacgaaccg cagcaatcca
     4921 gccccgcagg ggcggaattg gttttagccc agggcgtaag ccctgggtaa agtgaaaaaa
     4981 atcaacgagt cccttcaggg acggcacagt tctcacgcag acccttaaaa tcttgaagct
     5041 caaccttatg atagtggact agcttgcgtc gcgatcaatt gcagcttgaa agccgccctt
     5101 ttccgcaact tggcagtagc tgatggatac ccggattgta ctgtccgaaa ttggacatta
     5161 caatccgcag agggacatgg cctttcgtcc agaaaacccg cgattggctt aacctgctga
     5221 gaacacgcta actaacattg cctggaaaat ggttctccag ttgcacccat aaaggcgtaa
     5281 tggatatcgc ccgtccagaa ttgaagcgtc aaaagcgccg ccggcaaatc ctctggggca
     5341 gtatcgcgat tgtggttttg atcggtgtaa ccgttggaat ctcgcggctg aagccagccg
     5401 ccccgactgt cgatcgcagc accatttgga ccgacagcgt gaagcggggc tccatgcttc
     5461 gccaagtgcg cggtctgggt accctgatcc ccagtcatga ggacgtccgt cagattcccg
     5521 ccgaaactga agccacgatt atccgaatcg tcaaacttcc cgggtcgcta gtggaggccg
     5581 acaccgtctt ggtggagatg agcaatcccc aactcgaaca ggaggcgctg gacgcacagt
     5641 tacaagtgaa ggcggccgaa gccgaatacc agagcctgaa ggtaaagctc gcgagcgacc
     5701 tgatgacgca aaaagctgga gctgccacgg tcaacgccga ttatgccgag gcacagcgtc
     5761 aggcgcaaac cgataaagcg ctctatgatc tcggcgtaat ttccgggctc gctgccaagg
     5821 cttccgcggg caaagcccag gaactggtca cacgcaatga catcgaagat cagcgattgc
     5881 agatcaacca gaaagccatc gcctcccagt tggccgtgca acaagccaaa gtggagcaga
     5941 tgcgcgctct ggcggatctc aagcagaagc agttgcagcg cctgaaagtg caagccggag
     6001 ttgcgggtgt cctggtggat ttgccgttgc aggttggcca gcacgtgctc cctggagccg
     6061 agctggcgag gatcgtgcaa ccgaatcacc ttatggcgga gctgaaaatt ccagagacgc
     6121 aggcccgtga cgtccagatc ggcgaacccg cctcggtgga tacccacaac ggcgtcatac
     6181 cgggtgaagt aagccgggtc gatcccgggg tgcagaatgg aacggtaaca gtcgacgtga
     6241 agttgactgg cgaactgccc aaaggcgcgc gccctgatct gagtgtggat ggcaccatcg
     6301 atttggagcg actggagaac gtgctctacg tggggcgtcc agctttcggt caggaaacca
     6361 gcacaatttc catgttcaag cttgatcccg agggaaaggc agcgacgcgt gtgcaggtga
     6421 agaccggacg cgcctcggtc aacctgattc aaatcgagtc tgggcttcac gagggcgata
     6481 ccgtcattct ttccgacatg tcgcggtggg acaacaccga tcgcgtgcgg ttggagtagg
     6541 aaatgccgct gttcgctatt cgctattcgc ttttcgcttc tcgccgtgaa gtgagggctc
     6601 gcctgcaatt ggcgaacggg tgataccgaa aaacaggcca gaataatgcg agactccaat
     6661 agcgagcagc caagagggaa gagcgaatag cgaagagcga aaagcgagag tgaaaacaat
     6721 atgagcgatg caggaacccc tctaattgag attgaaaacc tcaccaaggt tttctacacc
     6781 gatgaagtcg agacgcacgc cctggctggc gttcatctca tggtgcgca
//
LOCUS       DASRKK010000251         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_311228, whole genome shotgun sequence.
ACCESSION   DASRKK010000251 DASRKK010000000
VERSION     DASRKK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311228"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFC15_05405"
     CDS             <1..301
                     /locus_tag="VFC15_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZM09629.1"
                     /translation="RYRVDETDDGFSGEEGTFTICSFWLVSALVEIGELARARGLCEK
                     LLSYASPLQLYAEEIDPATGRHLGNFPQAFTHLALISTATYLDRVLSGKDDSVWR"
     gene            448..1839
                     /locus_tag="VFC15_05410"
     CDS             448..1839
                     /locus_tag="VFC15_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM09630.1"
                     /translation="MLCLILIVGGLLIMMGQHDRSEALFYYFRLEDQVPETHLLRLIE
                     KHISFEFVRQQLKDSYSETGRPSIDPELLLRILLIGYLYGITSERRLVEELRMHLAWR
                     WFTGLGFDQEIPHHSTFSKNRHGRFQESKLFEQLFEQIVRQCVEVGLVQGKHLSVDGS
                     FVEANAAKQSRIPREQLGEAAQVHHTVRQYLREVEEQNPVEEPVHEQDQVSTTDPDST
                     YATKGGTPARLGYYDNYLVDNDSCVIVGVQGTAARMSQETVAAQGMLTRFAEWQGRAP
                     ESVAADTTYGNGEFLQWLADRNITPYMRTRDSIHRKRSPFFGPERFTYEPEHNRYICP
                     AGQPLNYGGRAYRNRAFNYIGTRKRCGACALKAQCTSAPFRSLNIHQQEPARQRAREL
                     ANTPEFARAQRQRKKVEALFAELKNQIGLRRLRLRRLKFVREQFFLAAVAQNIKRLVR
                     FLSQPPASTVLAT"
     gene            complement(1943..>2311)
                     /locus_tag="VFC15_05415"
     CDS             complement(1943..>2311)
                     /locus_tag="VFC15_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09631.1"
                     /translation="MYPGPSRDGRYCIQTDREHHYWLQPLDGAAAREVHGINPGERIL
                     EWHADSNSVFVAHLTGSDVDIYTLDLTNGRRKLWTHFSPAEKTALVGKSAVVITPDGA
                     HYVYQMQRIYSTLFLADGLQ"
BASE COUNT          513 a          643 c          692 g          463 t
ORIGIN      
        1 gcgctatcgg gtcgatgaga ccgacgacgg cttcagcggc gaggagggca cgttcacgat
       61 ctgctcgttc tggttggtct cggcgctcgt ggagatcggg gagctggcgc gggcgcgagg
      121 actgtgcgag aagctgctct cgtatgcgag tccgctccag ctctacgccg aggaaatcga
      181 tcctgcgacc ggccggcacc tcggcaactt tccccaggcg ttcacgcacc tcgcgctgat
      241 cagcaccgca acatatctgg accgggtcct ttccggcaag gacgactcag tgtggcgcta
      301 gcttttagag aatgatccct cggtgaaaac acgtttcgag tggaggcccg tcggaggatc
      361 tggtggaaat gcctgtgggt gttgaaaaag tcacgtcgat tagcgggatg agattcggca
      421 gaagcatgtt agaagagagc atcgcacatg ctttgtttga ttctcattgt cggaggcctg
      481 ctgatcatga tggggcaaca cgaccgctca gaagcgctgt tctattactt ccgtctggaa
      541 gatcaggttc ctgaaaccca cctgttgcgg ctgatcgaga agcacatcag cttcgagttt
      601 gtgcggcagc aactgaaaga cagctacagc gagaccggtc gtccctcgat tgaccccgaa
      661 cttctgttgc gcattctgct gatcgggtat ctctacggca tcaccagcga gcgaaggctg
      721 gtggaggaac tgcgcatgca tctggcgtgg cgctggttta ctggtctggg tttcgatcag
      781 gagatcccgc atcactcgac gttctccaag aaccggcacg ggcggtttca ggagtcgaag
      841 ctatttgagc agttgtttga gcagatcgtg cgccagtgtg tggaagtggg attggtgcag
      901 ggcaagcatc tgtcggtgga tggcagtttc gtggaagcga acgcggccaa gcagagccgc
      961 attccgcgcg agcagttggg ggaggcagcc caggtgcacc acaccgtgcg ccagtatctg
     1021 cgggaagtag aagagcagaa cccggtggag gagccggtgc atgagcagga tcaggtatcg
     1081 accaccgatc ccgactccac ctacgcgacc aaaggcggca cgccagcgcg tctcggttac
     1141 tacgacaact atcttgtgga taacgacagc tgcgtcattg tcggagtaca gggcacggcg
     1201 gcgcgcatga gtcaggagac ggttgccgcg caaggcatgc tgacccgttt cgccgagtgg
     1261 caaggacgag cacccgaatc ggtggcggcc gacacgacct atgggaacgg ggagttctta
     1321 cagtggttgg cggatcggaa catcaccccg tatatgcgga cccgcgacag tatccacaga
     1381 aagaggagtc cattcttcgg tcccgagcgt ttcacttacg aaccggaaca caatcgctat
     1441 atctgtcccg ctggccagcc actcaactac ggaggtcgag cttatcggaa tcgcgccttc
     1501 aactacattg gaacccgcaa acgatgcggc gcgtgtgcgc tcaaagcgca atgcaccagc
     1561 gccccttttc gatctctgaa catccaccag caggaacccg cccggcaacg cgcccgggag
     1621 ttagccaaca cgccagagtt tgctcgcgcc cagcgacaga gaaaaaaggt ggaagcgctt
     1681 ttcgccgaac tgaagaatca aatcgggctg cgtcgcttgc gcctgcggag actgaagttc
     1741 gtgcgggaac agttcttcct ggcggcggtg gcccagaaca tcaagcggct cgtgcgcttc
     1801 ctcagccaac cgccagcctc aacggttctc gccacttaga aaataaagag aggaccactc
     1861 gacaccattc ccacgcacag cacagggtca cttcaaacat agagtttttc aacagccacg
     1921 cctgttatca ctcatcgaca aactattgca aaccgtcggc gaggaagaga gtcgagtaaa
     1981 ttctctgcat ctggtagacg taatgagccc cgtccggcgt aatcacgact gctgattttc
     2041 ctactagcgc ggttttttcc gcaggagaga aatgtgtcca gagtttgcgg cgcccgttgg
     2101 tcaagtccag agtatagata tccacgtccg aaccggtaag gtgtgcgacg aaaacactat
     2161 tggaatcggc atgccattcg agtattctct cgccgggatt tatgccgtga acctcacgcg
     2221 ccgctgctcc gtcgaggggc tgtaaccagt agtgatgctc ccggtcagtc tggatacagt
     2281 agcgaccatc gcgcgaggga ccgggataca t
//
LOCUS       DASRKK010000252         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_604451, whole genome shotgun sequence.
ACCESSION   DASRKK010000252 DASRKK010000000
VERSION     DASRKK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604451"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFC15_05420"
     CDS             complement(<1..866)
                     /locus_tag="VFC15_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HZM09632.1"
                     /translation="MDIYEQIVELRRQGRRGAVATITNVRGSIPSFQTAKMLVRDDGS
                     IMGTIGGGCVEAEVWQVAREVMEEEKPRSLTFNLNNNPKYDTGLVCGGTLEIFVEPVL
                     PPALLYIFGAGHVAYNLYKVASIAGFDVVVVDDREAYANRERFPDAREVIARDFELAT
                     EQLSPPESSYIVIVTRGHRDDMRVLRWALNTRARYLGMIGSRRKTIAIYKELEKEGIP
                     ADKFRDVHAPVGLEIGAVTPEEIAVAIVAEMIAVRRHAPTDVASKMYSYPLRNPAAHD
                     EQDAAVEEGPTH"
     gene            972..2078
                     /locus_tag="VFC15_05425"
     CDS             972..2078
                     /locus_tag="VFC15_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09633.1"
                     /translation="MEAPVVVGLIAGAVSLIVALLGQIFNPIAQRKLENQKSELQGQI
                     EGQKAALQAIIENQKAELQKQIEDAKAESADRNSAKAARRDYEYEALKRLYTQVEPFF
                     LQLYEAVDSCYSRISALARTSRQHHLGTGHDSWIDQPGYFLRSTAYRLIVPAVHFRII
                     QRRITFVDFKLDQTLRLRYLLLRSLFRSYGDAFDFAGLSPKLDYDPVWEGREHLLMNP
                     VSYPQGLVAGDLDNTVEYLIHTQNDSTQPLTFGAFETEVRAIGNPQLADPDTGRKVED
                     LEELISLYCAFSPETRPILARMLLAQAGLCFLVMLTYRGQTSVDILRTGLEGFCADNE
                     MRKDLCWDAGASLGAIDIVRPYLNSCIDHIATAP"
     gene            2105..3127
                     /locus_tag="VFC15_05430"
     CDS             2105..3127
                     /locus_tag="VFC15_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM09634.1"
                     /translation="MKAVRIHEFGGPEVLKYEEIPDPVVRKDQVLVRVRACALNHLDL
                     WVRMGLPGVKLPHILGSDVAGEVVQCGDYISDVKPGTRVLLAPMVFCNHCEQCNNGRH
                     NFCPQFNVLGNGVDGGNCELIAVPRVNVIPIPDSLGFVEAASVPLVFLTAWHMLVGRA
                     QLRAGQTVLVLGAGSGVGSAAIQICKMLSAEVITTAGDEQKLAKARELGADHTIDHYK
                     QKIADEVKRITNRRGVDIVFEHVGQDTWKESVKSLKNGGTLVTCGATTGFQGDLDLRF
                     LFSRQLSLLGSYMGAMGELDEVLKHVFAGKLKPVVDRTFPLSETRAAHEYLAKSEQFG
                     KVVVTP"
BASE COUNT          675 a          941 c          991 g          691 t
ORIGIN      
        1 ttgtgggtgg gcccttcttc gacggcagca tcctgctcat catgcgctgc agggttacgg
       61 agagggtagg aatacatctt gctggcaacg tccgtcggcg cgtgccgccg gacggcaatc
      121 atctcggcca cgatggcaac cgcgatctct tccggcgtga ccgctccgat ctcgagaccc
      181 acgggagcgt gcacatctct gaatttgtcg gccggaatcc cttctttttc cagctccttg
      241 tagatcgcga tggtcttgcg acgtgagccg atcatgccaa gatagcgcgc gcgcgtattg
      301 agcgcccagc gcagcacacg catgtcgtcg cggtgtccgc gagtcacgat gacaatgtac
      361 gacgattcgg gcgggctcag ttgctccgta gccagctcga aatcgcgggc aatcacctcg
      421 cgggcgtctg ggaagcgctc acggttggcg taagcctcgc ggtcgtccac cacgactacg
      481 tcgaaaccgg caatggacgc aactttgtac aggttgtagg cgacgtggcc ggcgccaaag
      541 atgtatagca gcgcgggcgg aagcaccggc tcgacgaaga tttccagcgt gccgccgcag
      601 accaggccgg tgtcgtactt gggattgttg ttgaggttga aggtgagcga gcgcggtttc
      661 tcctcttcca tcacttcgcg cgcgacttgc caaacctcgg cctccacgca gccgccgccg
      721 atggtgccca tgatcgagcc atcgtcgcgc accagcatct tcgccgtctg gaacgacggg
      781 atcgagccgc gcacgttggt gatggtcgcg acagcgccgc ggcgtccctg gcggcgaagc
      841 tcaacgattt gctcgtagat atccatggag acagttcttt cattattgag atgcctgaga
      901 gacaagtcaa agggggggac ccaaagtcct gagcttccat tcgggtttgg tgccggggta
      961 aaattcacgc catggaagca cctgtggtag tcggtctcat cgccggcgca gtcagtctga
     1021 tcgtcgccct tctcggccag atctttaatc ccatcgcgca aaggaagctt gaaaaccaga
     1081 aatcagagtt acagggtcaa attgaaggcc agaaggcggc tctacaggcc ataattgaga
     1141 accaaaaagc cgagttgcag aagcagattg aggacgccaa agcagagtcc gccgaccgca
     1201 atagtgccaa ggccgctcgc cgcgactacg agtatgaggc gctcaagcgc ctctatacac
     1261 aggtcgaacc tttctttctg cagctctatg aggcggttga cagttgctat agccggattt
     1321 cagcgctggc ccgcacgtcg cgccagcacc atctggggac gggacacgat agctggatcg
     1381 atcaaccggg ttacttcctg cgctcgaccg cttaccgcct tattgtacct gcggtgcact
     1441 tccgcatcat tcagcgacgc ataacattcg tcgatttcaa gctcgaccag actttacgct
     1501 tgcggtacct cctgttgaga tccctgttcc gttcctacgg agacgctttc gattttgctg
     1561 gcttatctcc caagctcgat tacgacccgg tttgggaggg tcgcgagcac ctacttatga
     1621 atcctgtcag ctatccccag ggccttgtcg ccggggacct cgacaatacg gttgaatact
     1681 tgattcacac ccagaatgac agcacccagc cgcttacctt tggcgcattc gaaaccgagg
     1741 tgagggcgat cggcaatcct cagttggcag atcccgatac cggacgtaaa gtggaagatc
     1801 tggaggagtt gattagcctt tactgcgcgt tttccccgga aacccgtccg attctggcgc
     1861 gaatgttgtt ggcgcaggca gggctgtgct tcctcgttat gctgacctac cgcgggcaga
     1921 ccagtgtcga catcctccgc actggactcg aagggttctg cgctgacaac gagatgcgca
     1981 aggacctttg ttgggacgca ggcgccagtc tcggagcaat cgatatcgtg agaccttatc
     2041 taaactcctg tatagaccac atcgcaaccg caccgtagag tttagcttcg cgaggaatag
     2101 cacaatgaag gcagtccgca ttcatgagtt cggcggtcca gaggttctca agtacgaaga
     2161 gattcctgat ccagtcgtgc gcaaagatca ggttctggtg cgggtgcgcg cctgtgcgct
     2221 gaaccacctc gatctctggg tccgcatggg cctgccgggc gtgaagctgc cacacatcct
     2281 gggtagcgac gtggccggtg aagtggtgca atgcggcgac tacatcagcg atgtgaagcc
     2341 gggcacacgc gtgctgctgg cgcccatggt tttctgcaac cattgcgagc agtgcaacaa
     2401 cggacgccac aatttctgcc cacagttcaa cgtgctgggc aacggcgtgg atggaggaaa
     2461 ctgcgaactg atcgctgttc cccgtgtgaa tgtcatcccc atccccgatt cgctgggctt
     2521 cgtggaggcg gcgagtgtgc cgctggtctt tctcactgcg tggcacatgc tggtagggcg
     2581 cgctcagttg cgggcagggc agacggtgct ggttttgggc gctggttcgg gagtaggctc
     2641 ggccgcaatc cagatttgca agatgctgtc ggcggaggtg atcaccacgg ctggcgatga
     2701 gcagaagctg gcgaaagccc gcgaactcgg cgccgaccat accatcgatc attacaagca
     2761 gaagatcgcc gacgaagtga agcgcatcac caatcgccgc ggtgtggaca tcgtcttcga
     2821 acacgtagga caggacacat ggaaggagag cgtgaaatcg ctgaaaaacg gtggcacgct
     2881 ggtgacctgc ggagccacaa cgggctttca gggcgacctc gatctccgtt tcctgttttc
     2941 gcggcagctc tcgctgctgg ggtcgtatat gggcgcaatg ggtgagttgg acgaggtcct
     3001 caagcacgtt ttcgcgggca agctgaagcc ggtggtagat cggaccttcc cgctgagtga
     3061 aactcgcgcg gcacatgagt acctggcgaa aagcgaacag ttcggcaagg tggtggtcac
     3121 gccgtagctg gcgggcggct ttgcgtccgg ggctgaagcc ccggagtatt tacagcagtt
     3181 taggcgccgg cctgaaggcc ggctctaccc gaacatcgac ttattcagag ttcccctagg
     3241 tataattagt cgaaatgacc accgagtatc gtcctccaca cttcgataat cctgagcc
//
LOCUS       DASRKK010000253         5697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_800522, whole genome shotgun sequence.
ACCESSION   DASRKK010000253 DASRKK010000000
VERSION     DASRKK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800522"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(660..4202)
                     /locus_tag="VFC15_05435"
     CDS             complement(660..4202)
                     /locus_tag="VFC15_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZM09635.1"
                     /translation="MSLNGTTWTPIGPSPIDEGGITANGQVTAIAVNPNNPNIIYIGT
                     AWGGIWHTRNGGTTWTPIFDRAPALGIGEPGGLAIDPVNTNILYAGTSNRDGSQFSGE
                     ATQPPAGLFKSTDGGFSWVRLGSGYPSSAPSNASIFFSRVINVVIVDPANHQTVYLAS
                     NSGLFVSHDGGFNWTQGVTPTGDVRSLQLDTTSPPGARILYAGITSRGVFRSNDGGQT
                     WTSILSGTTPVLANELTTAGTTGGPTRSVGKFIIALAPPTSPPAPGGIQVLYATIEGR
                     PLNRPRQATDAPDPVGVFRSTDQGTTWTLQTPHNAGIPPGSGMPLNTQGGYSFHMAVD
                     PASPGDGLNDIIYLGAVGQARSTDSGQTFTGLNGLHADTHAWTFVPQPGPFSIAYCGN
                     DGGIFRCTSGINFASLNGNGFQTALFYNLDVKRDATASVTLGALQDNGIVTTKGAVAP
                     TWTRGAGGDGFDVAHDWQLATQAYGRSNATILQSTNDGVSYGGIDPPWLPAENNVYLA
                     AVATDPNNGGGVYASSNQNLWQSTNGGATWPNKVAIPRAATHVDIAPVNSNNVVVAVG
                     KQVFVSTDALVPGGLHLTDITRNLPGRFVGRVAFDPNDPATIYAVLGGLSGFPGGHVF
                     RTSLTATTWTDISPALDLPFNAIALDGSETPTTLYTGTDFGVLRSVDGGANWSVLDDI
                     HFPGAPVFDLAFQNGELRAATFGRGVFSFVKPTGPSIAVRPEHNLAFGIVCDGLHYLT
                     LEIYNVGVKDLLITSVQRLMGSSDFTVLSNPATPLILAPGEDIEFTVAYIPTGTATVE
                     AATIRIITNDPVSPVVDLLATAVHGFPKLATAIADSGNFGNVCLGSFRDEQLTLNNTG
                     ACRLLIGNITSTAIDFLAPGVASYPLAIGSGDSLDVTLRFAPSGLGAHAAAITVFSND
                     PSGAHTLKVSGFAPSPRLSVAVADTGSFGNVCIGSFRDLPLVLDNSGKCTLTVTGISS
                     SSPEFLPPEVISYPLTIGAGDSLEIPIRFQPTSIGAKTATITIDSDDPLGPRTITVSG
                     DVPAGKLAITGSNVFGGVNAHCCADRTISICNVGGCDLHVSSVAFKRKSSHWKLLNNP
                     FPATLHPGSCLGLTIRYKATEKCPRCCELLITSDDPAIPVKTVELLAYTIWNECECEK
                     GCDDCRKEGCEKGHSDRCRQGYPCCCEDDEPEDS"
     gene            complement(4226..4495)
                     /locus_tag="VFC15_05440"
     CDS             complement(4226..4495)
                     /locus_tag="VFC15_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09636.1"
                     /translation="MAKEHDDRPRGSHRGDDRHEHRREEPYDDPFEHREIENRRFQGG
                     LPPTPELYARAREQWYRLPGSVVRPSMDPVVGDSGTGQQDLPGEK"
     gene            5105..5413
                     /locus_tag="VFC15_05445"
     CDS             5105..5413
                     /locus_tag="VFC15_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09637.1"
                     /translation="MGGIRFSSAKEAGQSAMADKFHVSTSAPNRPCSCDVRIILYSLL
                     RCLQTAPASLGVAQYRWLVAGTTMLGDELSLLHRPEQIASSVFASILTSTWNGTAFGP
                     "
BASE COUNT         1141 a         1633 c         1741 g         1182 t
ORIGIN      
        1 tatgcacgaa tggagttagg gtcgtagctg cggccgcgct ctcttgaacg ataccgctga
       61 ccttgccgca agtcacagat tattcaatcc ggttcaacgg tggccccgct ttcttgtgac
      121 ttacggcgtg tcgtccgctc tcagtcggcg gtttgccgac tattcgctca agtggatttc
      181 cgccgcgaaa tgcgcccaat agcattcgta gaccctgggg cgtttagtag tacaaatctg
      241 cctggcgtct gtcattcatg ccgcttggct ggtacggtta cgatcttgtt gggtacgccg
      301 tgcatccgac gccaggcacc gggactgacc ccaacagttc cggtgaaagg aggggtgaag
      361 tggctttggt cggcgaaccc gcagacccgg gcgatttcca tcggttgagc aacgctgaag
      421 caactgcact cagtcgaccg tctgcatcga tccattgcat cgtcgtgatt cagattcgag
      481 gtaacgcttc aacatgggct cgctccatct gaaccagcag caggtatcgc agacacagaa
      541 atgcggaaat tgagcagttt cggaattgcc gaggcttcgg ccagttctct actccaaagt
      601 ctcagtggtt atcgataaat atctcacagt ccaccgatat cctcggtcgc ataatccctt
      661 taactatctt ccggctcgtc atcctcgcag caacaggggt atccctgcct gcagcgatcg
      721 gagtggccct tctcgcagcc ctccttacga cagtcgtcgc agcctttttc gcactcgcat
      781 tcattccaga tcgtgtacgc cagcaattcg accgtcttca ccgggatggc cgggtcgtcg
      841 ctggtgatca acagttcgca gcaccgcggg cacttctcgg tcgccttgta gcgaatggtc
      901 aggcctagac aggagcccgg atgcagcgtc gcagggaatg gattgttgag cagtttccag
      961 tgagagctct ttcgtttgaa cgcaacactg ctcacgtgga ggtcgcagcc gccgacgttg
     1021 cagatcgaga tggtgcggtc ggcacagcag tgagcgttca ctccgccaaa cacattcgat
     1081 ccggtaatcg ccagcttgcc cgccggcaca tcgccggaga ccgtaatggt ccgcggtccc
     1141 agcgggtcgt cactgtcgat cgtgattgtg gcggtcttcg ctccgatgga ggttggctgg
     1201 aagcggatcg gtatctcgag agagtccccc gccccaatcg tcaaaggata ggagatgacc
     1261 tccggaggga ggaactccgg tgacgacgaa ctgatgccgg tgaccgtgag cgtgcacttt
     1321 ccgctgttat ccagaaccag cggcaggtcg cggaacgatc caatgcagac attgccgaaa
     1381 ctgccggtgt cagcgacggc cacgctcagt cgcggcgacg gggcgaagcc ggagactttg
     1441 agcgtgtgag caccgctggg atcgttactg aacacggtga tcgcagcggc gtgggcaccg
     1501 agcccggacg gagcgaaacg cagcgtcacg tcgagcgagt cacccgaacc gatggccaga
     1561 ggatacgacg caactccagg agcgaggaaa tcgatcgctg tggaggttat gttgccgatc
     1621 agcaagcggc aggcgccggt gttattgagg gtgagttgtt catcgcggaa ggagccaagg
     1681 caaacattgc cgaaattgcc gctgtccgct atggcggtgg ccagcttcgg gaacccatga
     1741 acggccgtgg ccagcagatc cacgacggga gagacgggat cattggtgat gatccggatc
     1801 gtcgcggctt caacagtggc tgtgccggta ggtatgtaag cgactgtgaa ctcgatgtct
     1861 tcgccgggag caagaatcaa tggcgtcgcc gggttcgaga gcactgtgaa gtcggaggaa
     1921 cccatgagcc gctgcacgct ggtgatgagc agatctttga caccgacgtt gtaaatctca
     1981 agcgtcagat aatgaaggcc gtcgcagact attccaaacg caaggttgtg ctcaggacga
     2041 acggcgatcg aggggcccgt tggcttgacg aaggagaata cgccgcggcc gaaagtggca
     2101 gctcgcaact ccccattttg aaaagccagg tcaaacaccg gtgcgcctgg gaaatgaata
     2161 tcgtcgagaa cactccagtt cgcgcctccg tcgaccgagc gcagcacgcc gaagtctgtc
     2221 cccgtataga gagttgtagg cgtctcgctc ccatcgagcg cgatggcatt gaacggcagg
     2281 tcaagagccg gagagatgtc cgtccaggtg gttgctgtaa gagaagtccg gaagacatgc
     2341 cctcctggga aaccgctcag cccacccagc acggcgtaga tggttgccgg atcgttggga
     2401 tcgaacgcca cacgtccgac gaatcggccg ggcaggtttc gcgtgatatc ggtgaggtgc
     2461 aagcccccgg gcacgagcgc atccgtggaa acgaaaacct gttttccgac ggcgaccacg
     2521 acgttgttgc tgttgaccgg cgctatgtcc acatgggtgg cggctctagg gattgcaact
     2581 ttattcggcc aggtcgctcc accattggtg ctttgccaga gattctgatt gctgcttgcg
     2641 tacacgccgc cgccgttgtt tggatcggtc gcgacggctg ccaggtagac gttgttttcc
     2701 gccggcagcc agggtggatc aatccctcca taggaaacac cgtcgttcgt cgactgaagg
     2761 atggtggcgt tgcttctgcc gtaggcctgg gtggccaatt gccagtcatg cgcgacgtcg
     2821 aagccgtcgc ctccggcgcc cctcgtccag gtcggggcaa ccgcaccctt cgtggtgaca
     2881 atgccgttat cctgtagcgc gcccaaggtc acgctcgccg tcgcatcccg cttgacgtcg
     2941 aggttgtaga agagagcggt ctgaaagcca ttcccgttga gcgaggcgaa gttgatgcca
     3001 ctggtgcacc tgaagatgcc tccgtcgttg ccgcaatagg cgatggagaa tggaccgggc
     3061 tgcggcacaa aggtccatgc atgcgtgtcg gcgtgcagtc cgttcaaccc cgtgaaggtc
     3121 tggcctgaat cggtggaccg cgcctgaccg actgcgccca aatagataat gtcgttgagc
     3181 ccgtcgcccg gagaggcggg atcgacggcc atgtgaaagc tatacccgcc ctgcgtgttg
     3241 agcggcatgc cggagcccgg cggaataccc gcattgtgcg gcgtctgcag ggtccaggtc
     3301 gttccctgat cggtgcttct gaaaacgccc acgggatcgg gcgcgtcggt cgcttgccga
     3361 ggccggttga gcgggcgtcc ctcgattgtg gcgtacagca cctggatccc gcccggagcg
     3421 ggtggagagg ttggcggcgc cagcgcgata atgaacttcc cgacactgcg ggtgggaccg
     3481 cccgtcgtgc cggcggtagt gagctcgttc gcgagcaccg gcgttgtgcc gctcaggatc
     3541 gatgtccagg tttggccgcc atcgttggat cggaacacgc cacggctggt gatgccggca
     3601 tacaggatgc gcgcgcctgg aggcgacgtc gtatccagct gcagggagcg cacgtctccc
     3661 gtgggggtga caccctgggt ccagttgaag ccgccatcat gggaaacgaa cagtccggaa
     3721 ttggaagcga ggtagacagt ctggtgattg gccggatcga cgatgactac gttgatgacc
     3781 cgactgaaga agatgctggc attgctggga gcgctcgacg gatagcctga gccgagacga
     3841 acccaactga agcctccgtc ggtcgattta aaaagccccg ctggcggttg cgttgcttcg
     3901 cccgagaact gcgagccgtc ccgattgctg gttcctgcgt aaagaatgtt ggtgttaacc
     3961 ggatcaatgg cgagaccacc gggttcgccg atgccgagcg cgggcgctcg atcgaaaatg
     4021 ggtgtccagg tcgtgccccc attccgtgta tgccagattc cgccccatgc cgtgccgatg
     4081 taaatgatgt tgggattgtt cggattgacg gcaatggccg tgacctgacc attggctgtg
     4141 atcccgccct catcgatagg gctgggcccg atgggtgtcc aagtcgttcc gttcaggctc
     4201 atcgttctgt ccctctttta cccggctatt tctctccagg gagatcctgt tgaccggtgc
     4261 cagaatcacc gacgactgga tccatggacg gcctgacgac cgatccgggc agtcgatacc
     4321 actgttcgcg ggcgcgcgcg tagagttccg gggtcggcgg aaggccaccc tggaagcggc
     4381 ggttttcgat ctcgcggtgc tcgaagggat catcgtacgg ctcttctcgc ctgtgttcgt
     4441 ggcggtcatc gccgcgatgg gagccgcggg ggcgatcatc atgttctttt gccatgggtt
     4501 tcctcttcct cgtcgtcttc ttcggctttt tcggcattcg aaagtcacct ttcaatggct
     4561 aagctgatcg aagaagccga acatgttttc tccaagtgcg cccggcaagt ccaaacatca
     4621 ggacagaaat tgcggcccac aagagagcga aacccctctc tgcggagcca tctccggcat
     4681 caggagcgag gccgaacaac agctccatcc agtgaggcga aaggaatgcg agcgccagta
     4741 atgcagagct cgttagtccc agagcaagtt ccacccacac tttcgcgggc aggcggcctc
     4801 gcacgggcgg cgtttctggt cggcagaccg gacccatcag gaagcgattc tgttctctgg
     4861 aaacagcgga agattttttc tgcagaagta gcttcacgtt ggctcctttc gctcttgcag
     4921 gcattcaacg cagggccgat ggatgagcta agagaaacaa gaggccggga ttatacccca
     4981 cggtcaaatc ggattacaag attaaaaaat gggcttacct gatttctgtg tgcagaaccc
     5041 tctctgtcct ccagccgtca gaccccgcgg aagccttagc cggccctacg aaggttgtct
     5101 gatcatgggc ggcatcaggt tcagtagcgc caaagaggcc ggccagtccg ccatggccga
     5161 taagttccac gtttctactt ctgctcctaa tcggccctgt tcttgcgatg tcaggattat
     5221 cctctattcc ctgctccggt gcctgcaaac agcacctgca tcgctgggtg tggctcaata
     5281 ccgctggctg gtagccggaa ccacgatgct cggcgatgag ctgtccctgc tacatcgacc
     5341 agagcagatc gcatcttccg tattcgcttc tatcctgacc tcgacttgga acggaacagc
     5401 tttcggcccc tagcaattct gcagcaaaac agaatttgca acttacgggg gctatctgcg
     5461 atggtcgcct ctgtggccag aattactaca tggcgccaat tactactgct aaggtcggca
     5521 agtgtttacg aacgtgttcg cttcacttgg acgatgaacg cccgacgcgt ctgcgtgcag
     5581 gcataggctc ttggccagcc gcggtggagg cggctggaat gagcagacac gccaagcgga
     5641 aatcctcgaa actaaagcgg ccgaaaacaa agctcggcct cccagatctg gaccaca
//
LOCUS       DASRKK010000254         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_800565, whole genome shotgun sequence.
ACCESSION   DASRKK010000254 DASRKK010000000
VERSION     DASRKK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800565"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFC15_05450"
     CDS             <1..843
                     /locus_tag="VFC15_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM09638.1"
                     /translation="RMSVRYAAQVFRESFLKSAEAGRMGLPSVPLSQLYGTAAEYIEA
                     RGGKVHMRAAVDSVRADADGVQLCIGGEDVRADYAILATPFGGVEKLLPDGSEAEGLR
                     NQLRNLESSPITGIHLWFDREVTPLEHAVLLERTIQWMFQKSKILATRRDSAQSGSYL
                     ELVVSASKTLVEKSRAEIIEMAVRELAEFFPAVREATLTKATVIKEIHATFSPVPGGD
                     AFRPSHVSPWPKLFLAGDWTATGWPSTMEGAVRSGYGAAQALSGRKFLVPDLPAKGLM
                     RLFG"
     gene            1076..1870
                     /locus_tag="VFC15_05455"
     CDS             1076..1870
                     /locus_tag="VFC15_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09639.1"
                     /translation="MWRFYKHVIVSAFTEAWNATDKIIAKTAVFLFIVGLINREAVRW
                     LTTTWDGVSRWWSIAPIVALVLYRLLRKNYERFSTLEAEISESRVKLNQLMWPSNRPQ
                     IVFDRWDQVPANHPSAVPADPRVAGVFLQRGFYLINDGDAAHEVRVETFEVADGIWAS
                     SAMVPRIEGRGGGFALVWLETQKGIGSSLPNNVKWNLLDAMAKAEETNQGTSMYRGDY
                     SVPVSVIYRDGNNVWYRSRSTINYIPSQRRLQFGPITHESLPGGRP"
     gene            complement(1971..>2977)
                     /locus_tag="VFC15_05460"
     CDS             complement(1971..>2977)
                     /locus_tag="VFC15_05460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZM09640.1"
                     /translation="PRWKRCVQYVDRQLGEALGQAYVRKTFPPELKAATLDMTKEIED
                     AMVLRVQQLDWMSPQTKQQALNKLHAIRNKIGYPDKWRDYSSVVIARDDFYGNAERAE
                     EFENHREFNKVGKPVDRAEWEMSPPTVNAYFNPQMNDINFPAGVLQPPLYDAKLDDAP
                     NYGNTGSTIGHELTHGFDDEGRQFDAKGNLKDWWTTQDAEQFVKHADCVVDQYAKYVV
                     VDDIHINSKLTEGEDVADLGGTILAYIAWKNADKDKQLTNRDGLTPDQRFFVGFAQWA
                     CANDRPEDLRARAITDPHSPAKYRINGVVVNVPEFGTAFSCKAGQPMVKPADKVCKVW
                     "
BASE COUNT          595 a          816 c          897 g          669 t
ORIGIN      
        1 cggatgtccg ttcgctacgc tgcgcaagtc tttcgtgaat ctttcctgaa gtcggctgaa
       61 gcgggacgta tgggacttcc ctctgtcccg ctctcccagc tttacgggac ggcggcagag
      121 tacatcgaag cgcgcggtgg caaggtgcat atgcgggccg ccgtggattc ggttcgcgcc
      181 gatgccgatg gcgtgcagct ctgtattggt ggcgaagatg tgcgcgccga ctacgcgatt
      241 ctcgcgacac cgttcggcgg agtcgaaaag ctgcttccgg atgggtcaga ggcagaagga
      301 ttgcgcaatc aactgcgcaa tttggagtct tcccctatta ctggaattca tttgtggttc
      361 gatcgcgaag tcacgccttt agagcacgcg gtgctgctgg agcgcaccat tcaatggatg
      421 tttcagaagt cgaagattct tgcgacccgg cgggattcgg cgcagagcgg cagctacctg
      481 gagctggtgg tgagcgcgtc gaaaacgctg gtcgagaaat cgcgcgctga gatcatcgag
      541 atggcggtca gggaattggc agagtttttc cccgccgtgc gcgaggccac gctgaccaaa
      601 gcgacggtca tcaaggaaat ccacgcaacg ttctctccag tgccgggagg cgacgcgttc
      661 cgtccttcgc acgtcagtcc ctggccaaag ctcttcctcg cgggagattg gacagcaaca
      721 ggatggccgt cgaccatgga aggcgctgtg cgcagcggct atggcgctgc gcaagcgctg
      781 agcgggcgga agttcctcgt gcccgattta ccggcgaagg gattgatgcg gctgttcggg
      841 taacatcgat gagggatgtt atggtagggc cgaaatgaaa cgagtttcca agatacgttc
      901 ccaggctggc tcacaaaaac gccgcgccaa gcgcgccggg cagccttccc gcagcccgct
      961 gcggatcaca cctgcgcgcc acgggagtgg cctgaccgat gtgagcatag agcataatcg
     1021 gtatttcgcg gaacgatgac attacagttg taaaattcag cggaaaatgt acttcatgtg
     1081 gcgattctac aaacacgtca ttgtgtcggc atttacggag gcctggaatg cgaccgacaa
     1141 gatcattgcc aagaccgccg tttttctctt cattgtgggc ctcatcaacc gggaagccgt
     1201 aaggtggctg acgacaactt gggatggagt atcccgttgg tggagtatag ccccgatagt
     1261 tgccttggtc ttgtaccgcc tgctcaggaa aaactatgag agattctcga ccttggaagc
     1321 ggagattagc gagtcacggg taaagcttaa ccagctaatg tggccttcga atcggcctca
     1381 gattgtgttt gataggtggg atcaggttcc agcgaatcat ccctcagccg ttcccgcgga
     1441 tccgagggta gccggagttt tcttgcagag gggtttttat ctaatcaacg atggcgatgc
     1501 agcccacgag gtaagggttg agacgtttga ggttgcggat ggcatctggg ccagtagtgc
     1561 gatggttcca cggatcgagg gtcgcggggg aggttttgcc ctcgtgtggc tggaaacgca
     1621 aaaggggatc ggatcgtcct tgccgaacaa tgtgaagtgg aacttgttgg atgccatggc
     1681 aaaggccgaa gagaccaacc agggtacctc gatgtaccga ggggactaca gcgtaccggt
     1741 ttccgtgatc tacagggatg gcaacaatgt gtggtatcga agcagaagta ccataaatta
     1801 catacctagt cagcgccgtc ttcaattcgg cccgatcact cacgagtcgc tcccgggggg
     1861 aagaccttaa gcgctgccgc caattccaag cgcgacctgt ggttgcggta acctacgaat
     1921 acgttgtcag gtcatattta gcggaaggag acgacctccg agacaatccc tcaccacacc
     1981 ttgcacacct tgtccgcggg cttcaccatc ggctgtccgg ccttgcagga gaatgccgtg
     2041 ccaaattccg gcacattcac tactacgccg ttgatgcggt acttcgccgg agagtgcgga
     2101 tcggtgatgg cgcgcgccct caggtcttcg ggccgatcgt tggcgcacgc ccactgcgcg
     2161 aagcccacga agaaccgctg gtccggagtc aagccgtcgc ggttggtcag ttgcttgtcc
     2221 ttgtctgcat ttttccatgc gatgtaagcc agaatcgtgc cgcccaggtc ggccacgtcc
     2281 tcgccttccg tcagcttgga attgatgtgg atgtcgtcca ccacgacgta cttcgcatac
     2341 tggtccacca cgcagtccgc gtgcttgacg aactgctcgg cgtcctgcgt ggtccaccaa
     2401 tctttcagat tacccttggc gtcgaactga cggccttcat cgtcgaagcc atgggtcagt
     2461 tcatgcccaa tcgtcgagcc ggtgttgcca tagttgggag catcgtccaa tttggcgtcg
     2521 tagagcggcg gttgcagcac gccggcaggg aagttgatgt cgttcatctg cgggttgaaa
     2581 taggcgttca cggtcggcgg gctcatttcc cactccgcac ggtccaccgg cttgcccacc
     2641 ttgttgaact cgcgatggtt ctcgaattct tcggcccgct cggcgttgcc gtagaaatca
     2701 tcgcgcgcga ttacgaccga gctatagtcg cgccatttgt cgggatagcc gatcttgttg
     2761 cgaatggcat gcagcttgtt gagcgcttgc tgcttggtct gcgggctcat ccaatccaat
     2821 tgctgcacgc gcaacaccat ggcatcttca atttccttgg tcatgtcgag cgttgcggcc
     2881 ttcagctcgg gcggaaacgt tttgcgtaca tacgcttgtc ccagagcctc tcccagttgc
     2941 cggtccacat actgcacgca acgcttccag cgaggtt
//
LOCUS       DASRKK010000255         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_311509, whole genome shotgun sequence.
ACCESSION   DASRKK010000255 DASRKK010000000
VERSION     DASRKK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311509"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFC15_05465"
     CDS             complement(<1..915)
                     /locus_tag="VFC15_05465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM09641.1"
                     /translation="MKVLQLGKFYDPVVGGMETALKEICESLTDQVEFQVIVANTRCR
                     TEHENGKVRLTRVASMGKCLSLPVAPSFPLWAQRFDADLIHVHLANPLAELSALLANR
                     DIPVVAHFHSDVVRPLPPVLRNVYGRFLHAFYRRVNCIVVPSPSHIEISKFVPKYRDK
                     CHVVPYGIPLERFELDENGRKKADDLRDGLPTVLFVGRLVHYKGVEFLIRAMENVKAR
                     LWIVGTGPLEDSLRNLAREKGLADRVKFLGQVSEQDLIAHYHACDVFALPSITNQEMF
                     GLVQLEAMACRKPVISTNLPTGVPWVNQH"
     gene            complement(927..2108)
                     /locus_tag="VFC15_05470"
     CDS             complement(927..2108)
                     /locus_tag="VFC15_05470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM09642.1"
                     /translation="MPLPRVCVLCAISGLGGAEFSLLELVTRLRDSYEFHLIVPGEGP
                     LKERAESAGAKVWILPWPEAITSTGETATHAGVTKILHAAASLQPFTRRLSDLLEEIQ
                     PSVFVTNAVKAHIIGALARKPKNVPLIWYMRDGLEERILSRKLLALLSRRCDMAVCIS
                     EYVAAQFREYVSESVPTQVVYNIVDLNRFHPEALPPADLRKGPDEVWFGMVGAITPLK
                     GHDIFLGAAERVLDQQPNTFFVIVGNNPYLTEAGLRYEELLRRRMATSLVDRVKFVGF
                     RNDVPNVLSQLDVLVQPNRGPEGLGRSVLEAMACGVPVIAVDKWGPAELIEHGRSGLL
                     FPPRDAEKLATHMLTLARSKPLRKAMGKHGHAWIQQNLISKKLAGQFDGILACAIASP
                     Q"
     gene            complement(2095..>2671)
                     /locus_tag="VFC15_05475"
     CDS             complement(2095..>2671)
                     /locus_tag="VFC15_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZM09643.1"
                     /translation="EAYLLQRSIRWQQNGSRTLGHRLQENIEPLADDFQKVAALELQF
                     YMRNQLLRDADVFSMANSVELRVPFLDVRLLEAALAISPGYHFAGGSGKRITWRILRE
                     LTGEEIPHRRKMGFTFPWQQWLRRELKETITRTLREQRLYEPLLLDPAYGRQLLEGLE
                     REERLQSWSEVWSLFVLLNWQCRTGVECAVA"
BASE COUNT          601 a          799 c          728 g          543 t
ORIGIN      
        1 gtgctggttc acccagggca ccccagtcgg caggttggtc gagattaccg gcttgcggca
       61 agccatggct tcaagttgca ccaacccaaa catctcctgg ttggtgatcg atggaagcgc
      121 gaacacatcg caagcgtggt aatgggcaat tagatcctgc tcagatacct gtcccaagaa
      181 cttgactcgg tcggcgagtc ctttctcccg ggccaggttt cttagtgaat cttcgagggg
      241 acctgtaccg acgatccaaa ggcgcgcctt aacattttcc attgcccgaa tcaggaattc
      301 cactcccttg tagtggacta accgccccac aaaaagtact gttggcaggc catctcgaag
      361 gtcatcagct ttcttgcggc cgttctcatc cagttcaaag cgctccagcg gaatgccata
      421 aggcacgacg tgacacttgt cccgatactt ggggacgaat ttggagattt caatgtggct
      481 cggcgacggt accacgatgc aattcacgcg ccggtagaac gcatggagaa atcgcccgta
      541 cacgttgcgc aagaccggag gtagaggccg caccacgtcg gagtgaaagt gagcaacgac
      601 tggaatgtcg cggttagcca gcagcgctga caattcagcc aggggattgg ccagatgaac
      661 gtggatcaga tcggcatcga atcgttgcgc ccaaagcgga aatgacgggg caaccggtaa
      721 ggacagacat tttcccatgc tggcgacgcg ggtgagtcga accttcccgt tctcgtgctc
      781 tgtgcgacag cgagtgttgg caacaatcac ctgaaattcg acttggtcag taagactctc
      841 gcagatctct ttgagagcgg tttccattcc gccgacaacg ggatcgtaga attttccgag
      901 ttgcaaaact ttcatattat ggcctcttac tgcggggaag cgatggcgca ggcgaggatt
      961 ccatcaaact gccccgccaa cttctttgaa ataaggttct gctgaatcca ggcatggccg
     1021 tgcttaccca tggccttccg taaaggtttg cttctcgcca acgtgagcat gtgggttgcg
     1081 agcttctccg cgtcccgcgg tgggaacagc agaccgctgc ggccatgctc aatcaactcg
     1141 gccggccccc atttgtccac cgcgatgacg ggcacgccgc aggccatcgc ttccagaacc
     1201 gaacgcccca gcccttcggg gccgcgattc ggttgcacca ggacatcgag ttgggaaagg
     1261 acattgggca cgtcgtttct gaagcccacg aatttcaccc ggtccaccaa cgaagtcgcc
     1321 atcctgcgcc gcagtagttc ttcatagcga agcccggcct ccgtaagata gggattgttc
     1381 ccaacgatca cgaaaaacgt attcggctgt tggtcaagaa ctctctccgc cgctccaaga
     1441 aaaatgtcgt gtccttttaa cggtgtgata gcgccgacca tgccgaacca gacttcatcg
     1501 gggcccttgc gcaaatctgc cggtggcaga gcctcaggat ggaagcgatt caaatcgaca
     1561 atgttgtata ccacttgcgt cggcaccgat tcggaaacgt attcccgaaa ctgagcggcc
     1621 acgtactccg agatacaaac cgccatatcg cagcgccgtg acagcagcgc cagcaacttg
     1681 cgcgaaagaa tacgttcttc taagccatcg cgcatatacc aaattagtgg gacgttcttc
     1741 ggcttgcggg caagagcgcc gatgatatgg gccttgacgg cgttggtaac gaacaccgac
     1801 ggctgaattt cctctagcaa gtccgaaagg cgccgcgtga aaggctgcag gctggccgcg
     1861 gcatgcagga tcttcgtcac tccggcatgc gtggcggttt cgccggtgct ggtaatggct
     1921 tcgggccaag gcaatatcca taccttcgcc ccagccgact cggctctctc cttcagaggc
     1981 ccttcaccag gaacaatcag atgaaattcg taggaatcac gcaggcgggt caccagttcc
     2041 agcaggctga actccgctcc gcccagcccg gaaatcgcgc acagcacgca gaccctaggc
     2101 aacggcacat tccacccccg tcctgcactg ccaattcagc agcacgaaga gtgaccatac
     2161 ctcggaccac gactgcagcc gttcttcacg ctccaagcct tccagcaact gccttccgta
     2221 ggcgggatcg agcaacaggg gctcatagag ccgctgctct cgcaaggtgc gcgtgatcgt
     2281 ctccttcaac tcacggcgaa gccactgctg ccaggggaag gtaaatccca tcttgcgccg
     2341 gtggggaatt tcctcgcccg taagctcgcg cagaatccgc caagtgattc gctttccact
     2401 cccgccggcg aagtgatatc ccggtgaaat ggcaagcgcc gcttcgagca gccgcacatc
     2461 gaggaagggg acgcgcaact ccacggagtt ggccatgctg aacacgtcag catctctcag
     2521 cagctgatta cgcatgtaga attgcagctc cagggcggca accttctgaa aatcgtcggc
     2581 cagaggttcg atattttcct gaagacggtg ccccagtgtc ctcgaaccgt tctgctgcca
     2641 gcgaatgctg cgctggagca ggtaagcctc c
//
LOCUS       DASRKK010000256         8021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1093851, whole genome shotgun sequence.
ACCESSION   DASRKK010000256 DASRKK010000000
VERSION     DASRKK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093851"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFC15_05480"
     CDS             <1..265
                     /locus_tag="VFC15_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09644.1"
                     /translation="NRVADPLALMGVFHFIYTVMIALSAVCGILGLMAGLALLAERRS
                     GRTLALVAGFLSLSNIPLGTTLGIYTLVMLLPIAATQRYGRSD"
     gene            703..876
                     /locus_tag="VFC15_05485"
     CDS             703..876
                     /locus_tag="VFC15_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09645.1"
                     /translation="MSPAARKRISEAMKQRWAKWKGKSSPKQAKATPKKTAVRRPMSA
                     AAKKKLSALMKAR"
     gene            904..1695
                     /locus_tag="VFC15_05490"
     CDS             904..1695
                     /locus_tag="VFC15_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZM09646.1"
                     /translation="MSSSSFGKHASGATSGQSAVPEPSFAERARTLMHSGRIGSLSTL
                     SRKQPGFPFGSLMPYGLDRQGRPIFLISTMAMHTQNLQADPRASLFVTEPDASGDALG
                     SSRVTLIGSAHRIPEPEVTDARAVYLTGYPDSKYWVDFDDFFFYRLDVLDVYYVGGFG
                     VMGWVTASDYSRAQSDPLAGHKKDIMQHMNADHKDALILLAKRFAGIDAQQAEMTSVD
                     RLGFHLRLKTQDGMKGTRIAFLREVSDPTQTREVLVEMVKQARQG"
     gene            1743..3293
                     /locus_tag="VFC15_05495"
     CDS             1743..3293
                     /locus_tag="VFC15_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM09647.1"
                     /translation="MEESAKPDLKAGFPFDKLKDGGMILGHVDGEDVILARRGDELFA
                     VGAACTHYHGPLAQGLLVDETVRCPWHHACFSLRTGEALRAPALDPIACWRVERIGDE
                     VYVREKLAEAHPKATLGTETSHKLPSSIVIIGGGAAGLAAADMLRREGYDGPLTMISA
                     DDSAPYDRPNLSKDFLAGTASDDWIPLRSSDFYTDRRIELVLGSRVSALDLQQKRVQL
                     ENGKTCGFEALLIATGAEPVRLKIEGATDSQVTYLRTFADSKAIVAKAASAKRVAVVG
                     SSFIGLEVAASLRTRGIEVDVVAPDRQPLERVMGPEVGRFIRGVHEAHSVLFHLGQTV
                     KHIDGRRLTLSDGAEFEADFVVLGVGVRPSLAVAEQAGLAIDRGIVVSEYLETSVPGV
                     FAAGDVARWPDPHSGERIRVEHWVVAERQGQVAARNMLGYREPFNAVPFFWSQHYDVV
                     INYVGHAEEWDAIEIEGELDARNCAVAYKKGDRTLAVATISRDLQSLQAEAAMEAALG
                     RQNVESRA"
     gene            complement(3316..4020)
                     /locus_tag="VFC15_05500"
     CDS             complement(3316..4020)
                     /locus_tag="VFC15_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZM09648.1"
                     /translation="MCGRYRLSRRKQLIAEYFETDNEVDWEPRYDIAPSQPVGIVRQD
                     PSRPERHFSVARWGLIPSWATDASIGFKTINARAETVTNKPAFRDAFVSRRCLLPADG
                     FFEWRRCGREKQPFHFGMQDDSLFAFAGLWDRWRDPTGTVIESCSILTTTPNSLLADV
                     HDRMPVILPQESYDLWLDPGFKDLEALAEFLVPFDASQMRSFPVSTRINAVANDDPDC
                     VEPWHRDLPAQSALFG"
     gene            4077..4772
                     /locus_tag="VFC15_05505"
     CDS             4077..4772
                     /locus_tag="VFC15_05505"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZM09649.1"
                     /translation="MTGKRRVALIGDYNPAVIAHQAIPEALRLAGLQTGDDVEGVWLY
                     TASIVDPAAQLRDFAGIWCVPASPYASSDGAVEAIRYAREQGRTFLGTCAGFQHALLE
                     YVRNVCGLREAAHAEDNPEADCRVISPLSCSLVEKSGEILLIEGTQLYQAYGKARITE
                     GYHCNYGLNREYEPLLFGGSLRPTAHDLSGEVRAVELAGHPFFVATLFQPERAALRGE
                     VPPVVASFVAALQ"
     gene            complement(4803..5189)
                     /locus_tag="VFC15_05510"
     CDS             complement(4803..5189)
                     /locus_tag="VFC15_05510"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HZM09650.1"
                     /translation="MQAVVLAPTFYSALAVSVLLLHALYILWVVFGALLTRSRPILRW
                     LHVGSLVWGILTELLPWPCPLTVLENWLEGNAGVEPEKSGFLLHYLDKLVYPNISSTA
                     LTIAAVIICVLNLAFYGRQVWIARVR"
     gene            5282..6343
                     /gene="dinB"
                     /locus_tag="VFC15_05515"
     CDS             5282..6343
                     /gene="dinB"
                     /locus_tag="VFC15_05515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZM09651.1"
                     /translation="MRKIIHVDMDAFYASVEQRDDPQLRGKPVIVAWLGNRSVVCAAS
                     YEARSFGVHSAMPAIRAERLCPDAVFVPPDFTRYREVSRRVREIFKRHTELIEPLSLD
                     EAYLDVTENKTGLATATLVARTIRQQIREELNLTASAGAAPNKFLAKLASDWRKPDGL
                     FAIRPEDIDAFLLPLPVGRLPGVGKVTGEKLAKLGAQTVRDLRALDLPVLQHHFGRCG
                     QRLHELSLGIDHNPVVPDRPTQSISVEDTFEHDVLLPETEPMIRGLAEKLWSASRKEP
                     RTARTVVLKLKTSDFKILTRSHTPGSPPASCDELTDIALKLRERVDLGPKQRYRLVGV
                     GLSNFQEPEGTAAQPVLFE"
     gene            complement(6398..7123)
                     /locus_tag="VFC15_05520"
     CDS             complement(6398..7123)
                     /locus_tag="VFC15_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM09652.1"
                     /translation="MSSSGLSTVRKAKQHQPAVKRLRVLISAASSITRAGLERLLEKQ
                     PSLHLVGAISSVGDLPQAIAENDPDAVLLHLEAHSAETKWEELIAFEIPIVLLADEVD
                     LVSATAALAGGVQAVLLGDATGTELAAAAHSAAAGLLTLSGDLAGLVRQSLLAHSREE
                     ADESGGRPDPIADASPEHLTLREREVLEMMMEGLSNKEIAAHLNISAHTVKFHISSIL
                     GKLGASTRTEAAAIGLRRGLITI"
     gene            complement(7126..>8021)
                     /locus_tag="VFC15_05525"
     CDS             complement(7126..>8021)
                     /locus_tag="VFC15_05525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZM09653.1"
                     /translation="WEAISNHTTTLAEQTGKAIVAVDAGHRVVASGVHWRPGIVVTAS
                     HLVRRADEVSVLLPDGGSVKGTVAGHDSTTDLAAVRIENSSSLATLPLASDAKVGELV
                     LAVARSSRGELSASVGIVARVGGPWHSWRGGQIDRLLRPDVRLYPGQSGSALVNGRGE
                     LLGVNSSVLARESVITLPIETVDRVLKELLERGHIARPYLGIAMQEVPLPQEWRATAG
                     ANQEHGLLVMHVAPDGPAKQAGITLGDLVVSVDAQPVRGFRHFHHTLALKRPGEVVKL
                     RVLRAGNATEAEVSLGDRPRR"
BASE COUNT         1715 a         2349 c         2377 g         1580 t
ORIGIN      
        1 caatcgcgta gctgacccgt tagcactgat gggtgttttc cactttatct acacagtaat
       61 gatagctttg tcggctgtgt gtggaattct ggggctcatg gctggactgg ctttacttgc
      121 agaacggcga tcaggccgca ctctcgccct cgttgcaggg tttttgtcgc tctcaaatat
      181 tccgctgggt acaactctag ggatttacac gctcgtaatg ttgcttccaa tagcagctac
      241 gcagcgttac ggtcgttccg attgacgcct gctgcccggt gccatgcgca tgacgcctgt
      301 aaggctgagg tgttagagat atgtgtttaa cctgggcggc acacgttgga ttgacggagg
      361 cggctggccg tacccggagc tggcagacag ccgccaggac ggctgggcat actcgtacat
      421 ttacccgtcc tcaaaaggca cgagcgatcc gccctgacaa gcccaaagat caccgaggtc
      481 ccagatgagt caagctccgt ctggatctaa agacccgcct gctacaattc tcgccgcacc
      541 attcgagagg aacccttgga catccaaacc atagtttcag atctcaaggc cgaacgtaat
      601 cggattgacc aggccatttc cgcacttgaa ggacttactt caactgcacc ccgacgtgga
      661 cgtccgccaa aggcgacatc gcctggcggg agccgtcgcc gcatgagccc agccgcccgc
      721 aagcgcatct ctgaggccat gaagcagcga tgggcaaaat ggaagggaaa gtcctcgccg
      781 aagcaagcca aggcgacgcc taagaagacc gccgttcgcc gtccgatgag cgcagccgcg
      841 aaaaagaaat tgtcagcact catgaaagcg cgttaggccg agaagaaaaa ggcaaaagca
      901 taagtgtctt cctcatcctt cggaaaacac gcgagcggcg ccacgagtgg ccagtctgca
      961 gttcccgagc cttcgttcgc ggagcgagca cgcacgttga tgcactcggg ccgaatcggg
     1021 agtctctcca ccctatcgcg gaaacaaccg ggttttcctt tcgggtcgtt aatgccttac
     1081 gggctggaca ggcagggccg ccccatcttc ctcataagca cgatggcgat gcatacgcag
     1141 aatctgcagg ccgatccccg cgccagcctg tttgtgaccg aacctgacgc aagtggcgac
     1201 gcactcggct catccagagt cacattgatc ggaagtgccc acaggatacc cgagccagaa
     1261 gtgacggatg cccgtgcggt ctatctgacg ggctacccag atagcaagta ttgggtcgac
     1321 ttcgacgatt tcttctttta ccgattggat gttctggacg tctattacgt gggcggcttt
     1381 ggagtgatgg gatgggtgac tgcttcggat tactcccggg cacaatccga tccactggct
     1441 gggcacaaga aagacatcat gcagcacatg aacgccgacc acaaagatgc gctcatcctc
     1501 ctcgcaaaac ggtttgccgg catcgatgca caacaggcgg agatgacttc ggttgatcgc
     1561 ctaggcttcc acctgcgcct gaaaacccag gatggcatga agggaactcg gattgcgttc
     1621 ttgcgggaag tgagcgatcc cacgcagacc agagaggttc tcgtcgagat ggtaaagcag
     1681 gctcgtcaag ggtaaggcga ggtttgtgtg acggccaatc gtgaaaggag ccttcggaac
     1741 ccatggaaga gtccgccaaa cccgatttga aagctggatt tcccttcgac aagctgaaag
     1801 atggcgggat gattctgggc cacgtggacg gcgaagacgt tatcctggcg cgtcgtggcg
     1861 atgaactctt cgcggtaggt gcggcctgca cccactacca tggcccgctc gcccagggtc
     1921 tgctagtcga tgaaacggtg cgctgtccgt ggcaccacgc gtgtttcagt ctcagaactg
     1981 gcgaagcact gcgcgcgcct gcactagacc cgatcgcgtg ttggcgggtc gagaggatcg
     2041 gcgacgaggt ctatgtacga gagaagctcg cagaagcgca cccgaaagca acgctcggta
     2101 cagagacttc gcacaagttg ccatcctcga tcgtgattat cggcggtggt gctgcagggc
     2161 tggccgctgc ggacatgctc cgtcgtgaag gctacgatgg cccgcttacc atgatcagcg
     2221 cagacgactc tgctccttat gatcggccga acctctccaa agactttctc gccggaaccg
     2281 catcggacga ctggatccct ctgcgatcgt ctgacttcta caccgaccgt cgaattgaac
     2341 tcgtgctcgg ttcgcgagtg tcggcgctgg acctccaaca gaagcgcgtc caacttgaga
     2401 atggcaagac atgcggattt gaggccctac tgatcgcgac gggcgccgaa cccgtcagat
     2461 tgaagatcga gggcgccacg gattctcagg ttacctatct gcgcacgttt gccgacagta
     2521 aagcgattgt agccaaggcg gcatctgcca agcgcgtagc ggttgtcggg tccagcttca
     2581 ttggccttga ggttgcagcg tccttgcgca cacgcggtat tgaagtggat gtggtagctc
     2641 cagatcgcca gcccctggaa cgtgtgatgg gaccagaggt cggtcgcttc attcggggag
     2701 tgcatgaagc ccacagtgtc ctctttcact tgggtcagac cgttaagcac attgacggac
     2761 gtagattaac gttaagcgat ggagcggagt ttgaagcgga cttcgtggtc cttggcgtgg
     2821 gcgtccgtcc atcgctagcc gtcgccgagc aagccggtct ggcgatcgac cgcggcatcg
     2881 tcgtcagcga gtatctggaa accagcgtgc ctggcgtctt cgctgccggc gatgtcgccc
     2941 ggtggcccga tccgcattcc ggggaacgaa tccgcgtcga gcactgggtc gtagccgagc
     3001 ggcagggtca agtcgcggct aggaacatgc tgggctatcg cgagccattc aacgcagtgc
     3061 cgttcttttg gagtcagcac tacgatgtcg ttattaacta tgttggccat gccgaggagt
     3121 gggatgccat cgagattgaa ggagagctcg atgcgcgaaa ctgtgccgtc gcttataaga
     3181 agggcgaccg cactctcgcg gtagcaacga tttcacggga tctgcaaagt ctgcaggctg
     3241 aggcagcgat ggaagccgcg ctggggcggc agaacgtaga gagtcgagcg tagatcgaat
     3301 ggtgccacct gtgacctacc cgaagagcgc actctgagcc ggcaaatctc tgtgccaagg
     3361 ctcaacgcaa tccgggtcgt cgttcgccac cgcgttaatt cgagtgctta ctggaaagct
     3421 ccgcatttgc gaggcatcga acggcacgag aaactccgcg agcgcctcca aatccttgaa
     3481 gccgggatcg agccacagat catagctctc ttgcggcaga attaccggca ttcgatcatg
     3541 tacgtccgcc agcagcgagt tcggagtggt ggtcagaatc gagcaagact caatcacagt
     3601 gccggtcggg tctctccatc gatcccacag accggcaaac gcaaacaggg aatcgtcctg
     3661 catgccgaag tggaagggct gcttctctct cccgcaacgc cgccactcga aaaagccgtc
     3721 ggcaggaagt aagcaccgac ggctcacgaa ggcatcgcgg aacgctggct tatttgtaac
     3781 cgtctccgcg cgcgcgttga tcgtcttgaa gccgatactg gcgtccgtgg cccaggacgg
     3841 aatcaggccc catcgagcga cagaaaagtg tcgttccggt ctcgatggat cctgccgaac
     3901 gatacccact ggctgagatg gagcgatgtc atagcgaggc tcccaatcga cctcattgtc
     3961 ggtctcgaag tattccgcga tcaattgctt gcgtctggac agacgatagc ggccacacat
     4021 tactggctcc tggagtccaa ttctaatgga actgccaacc ttgtattctt ctgtacatga
     4081 ctggcaaacg gcgcgtcgct ctcataggcg attacaaccc cgctgtcatc gcgcaccagg
     4141 ccattcccga ggcactgcgg ttggccggtc tccaaaccgg agacgacgtt gaaggtgtct
     4201 ggctctatac cgcttcgatc gttgacccgg cggcccaact gcgcgatttt gcgggaatct
     4261 ggtgcgtacc tgccagtcct tacgcgagtt ccgacggcgc tgtggaagcg atccgatacg
     4321 cccgggagca ggggcgaacc ttcctcggaa cctgcgccgg ctttcagcac gcgctactgg
     4381 aatatgtccg caacgtgtgc ggcctgcgcg aagccgccca cgccgaggac aatcccgaag
     4441 ctgactgccg cgtgatttca ccgctgtcct gttcactggt ggagaagagt ggcgaaatcc
     4501 tactgatcga aggcacgcag ctctaccaag cctatggaaa ggcgcgaatc accgagggct
     4561 atcactgcaa ctatggcctc aaccgtgaat atgagccgct actgtttggc ggcagccttc
     4621 gacccacggc gcatgacctc tccggcgaag tgcgcgcggt ggaactagcg ggacacccat
     4681 tctttgtagc cacacttttt cagcccgaaa gggcggcgct tcgcggggaa gtcccaccgg
     4741 tggtggcgtc gtttgtcgct gctctccaat agatcgccaa aacctggggg ctggcgcggc
     4801 cgctagcgga ctcgagcaat ccagacttgc cggccataga acgcaagatt cagaacgcag
     4861 atgattaccg cagcaatcgt tagcgcggtt gaagaaatat tcggatagac cagcttgtcc
     4921 aagtaatgga gcaggaaccc actcttttca ggctcgactc cggcgttgcc ttcgagccag
     4981 ttctccagca cagtcaaagg acacggccaa ggaagcagtt ccgtaaggat tccccacaca
     5041 agtgaaccga catggagcca acgaagaatc gggcgcgagc gtgtcagcaa cgcaccgaaa
     5101 acgacccaca aaatgtatag cgcatgcagg aggaggacag aaaccgcgag tgcggaatag
     5161 aaagtcggag caagaactac tgcttgcatc tgtagaattt accgcaagga aagttaacga
     5221 tccgcccgac acgccgttca ccccgttctg agagcccgaa tagtattgtt gccagaagat
     5281 tgtgcgaaaa atcatccatg tcgacatgga cgcgttttac gcttcagtcg agcagcgcga
     5341 tgatccgcag ctgcgtggca agcccgtgat cgttgcttgg ctaggcaatc gctctgttgt
     5401 gtgcgccgct tcgtacgagg ccaggagctt cggcgtgcat tctgccatgc cagcaatacg
     5461 agccgagcgc ttgtgtcccg atgcggtctt cgtgcctccg gatttcacac gctatcgcga
     5521 ggtctcgcgc agggtgcggg agatcttcaa gcgtcataca gagctaatag aaccactgtc
     5581 tctcgacgaa gcctatctcg acgtcaccga gaacaagacc ggcttggcga cagcaacctt
     5641 ggtggcgcgc acgatccggc agcaaatccg cgaggaattg aatctgacag cctctgcggg
     5701 agctgcgccc aacaaattct tggccaagct cgcctcggac tggcgcaagc ctgatgggct
     5761 gtttgcaata cgacctgagg acatagacgc gtttctgctg cctttgcccg ttggacgctt
     5821 gcctggcgtc ggcaaagtta cgggagagaa gctggcgaaa cttggagccc agacggtgcg
     5881 ggacctacga gccctcgact tgcccgtact ccaacaccac ttcggccgat gcggtcagcg
     5941 cctgcacgag ctgtcactcg gtatcgacca caacccggtt gttcctgaca ggccgacaca
     6001 atcgatatcg gtcgaggata cttttgaaca tgacgtgctg ttacccgaaa ccgagccgat
     6061 gattcgggga ctcgcagaga agctttggtc ggcttcgcga aaagaaccgc ggacggcacg
     6121 cacggttgtc ttgaagctga agacaagcga cttcaagatt ctgactcgca gccatacgcc
     6181 gggctccccg cccgcctctt gcgatgaact aacggacatc gccttgaagc tgcgagaacg
     6241 ggttgatctc ggtccgaagc agcgctatcg gctcgtcggc gttggtttga gcaacttcca
     6301 ggaaccagaa ggcactgccg cgcagccggt cctcttcgag taacaccttt gtcaagcgag
     6361 aatgtttgcg ctgaacagcc ccgcccaccc tcagacttta gatggtgatc agtccccggc
     6421 gaagaccaat cgcagccgcc tccgtacggg tggacgcgcc cagcttgccc aaaatggagc
     6481 tgatgtggaa cttcactgta tgggcggaga tgttgagatg ggcggcaatc tctttattcg
     6541 acagaccctc catcatcatc tcgagcactt cccgttcccg aagtgtgagg tgttcaggag
     6601 aagcgtcggc gataggatcg gggcgtcccc cagattcgtc agcctcctcg cgcgaatgag
     6661 cgaggagact ctggcgtacg agaccggcca gatcgcccga aagcgtaagc aatccggccg
     6721 cagcgctgtg ggcggctgca gctaactcag ttccagtggc gtcacccaag agtacggctt
     6781 gcactccgcc agcgagagcg gctgttgcac tcacaagatc gacttcgtcg gcgagcagca
     6841 cgataggaat ttcgaatgcg atcaattctt cccactttgt ctccgcggaa tgcgcttcga
     6901 ggtgcaggag caccgcgtcg ggatcgttct ccgcgattgc ctgtggcagg tcgccgacac
     6961 tactgatcgc acccacaaga tgcaagctgg gctgcttctc gagaaggcgc tccaaccccg
     7021 cgcgggtgat cgacgaggca gcggagatca gcacgcgcaa ccgtttgacc gccggctgat
     7081 gctgctttgc ctttcgcaca gtgctgagcc cgctgcttga catggttatc gccttggccg
     7141 gtcgcccagc gaaacctccg cctcagtggc gtttccagcg cgaagcacac gcagctttac
     7201 cacttctcca gggcgtttca gcgcgagcgt atggtggaag tggcggaatc cgcggaccgg
     7261 ttgcgcgtcc acgctgacga caaggtcgcc cagagtgatg cctgcctgtt tagctggtcc
     7321 gtcgggcgcg acgtgcatga ccagcagacc atgctcctga ttggctccgg cagtggcacg
     7381 ccactcttgt ggcaagggaa cttcctgcat ggcgatgcca aggtagggcc gcgcgatatg
     7441 gccacgctcc agcagttctt tgagcacccg atcgacggtt tcgatgggca aagtgatgac
     7501 cgattcgcgt gcgagcacag aactgttcac gcctaacagt tcgccgcgac cgttcacgag
     7561 tgcgctgcca gactgaccag gatagaggcg gacgtcgggg cgcaagaggc ggtcgatttg
     7621 acccccgcgc cacgaatgcc aaggtcctcc gacacgggcg acgattccga cgctcgcaga
     7681 gagttcgcct cgactagaac gcgcaaccgc cagcaccaac tcgccgacct tcgcgtcgga
     7741 ggcgaggggc aaggtggcga gactcgacga gttttcaatg cgtacagccg caaggtcagt
     7801 cgtgctgtcg tggccggcaa ccgttccctt cacgctgccg ccgtcgggaa ggaggacgct
     7861 gacttcgtcg gcccgtcgca cgagatggct ggcggtgacg acaatgccag gcctccaatg
     7921 aacgccgctg gcgacgacgc gatggccagc atcgacggcg acgatagcct ttcctgtctg
     7981 ctcggcgagc gtggttgtgt ggttcgagat ggcttcccag a
//
LOCUS       DASRKK010000257         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_996230, whole genome shotgun sequence.
ACCESSION   DASRKK010000257 DASRKK010000000
VERSION     DASRKK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996230"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFC15_05530"
     CDS             <1..563
                     /locus_tag="VFC15_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09654.1"
                     /translation="ATETVVLLSPQGQLFSQAVAQELVSRERVVLICGRYEGVDERVS
                     EYLADREISVGDFVLSGGELGAAIIVDTMTRLTPGALGNAASAQQESFTPTVVDAGER
                     ADNAPPSSTCGSGGLLDYPHYTRPAEFRGMSVPEVLMNGNHDEIRRWRRRRALEKTLA
                     YRPDLLLGAELSKEDRKLLEEIEGRE"
     gene            699..1064
                     /gene="rplS"
                     /locus_tag="VFC15_05535"
     CDS             699..1064
                     /gene="rplS"
                     /locus_tag="VFC15_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZM09655.1"
                     /translation="MSNSPIIDRLIAKTRRTDLPEFKAGDTVRVHVKIKEGEKERVQV
                     FEGLVIAKSKGTQPSFIVRKMSFGQGVERIFPLNSKVIEKIEVLRSAHVRRAKLYYLR
                     ALRGKAARLREVDVRTKKA"
     gene            complement(1169..1777)
                     /locus_tag="VFC15_05540"
     CDS             complement(1169..1777)
                     /locus_tag="VFC15_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09656.1"
                     /translation="MGKELQCSVRSGGKRSDGKALLETNEIVFRGDLRLKIPLASIKS
                     VVARNGELHLKWLENSTVFELGEQAEKWAHKILHPKSTAEKLGIKPGSRISALRLSET
                     EVLKDARKAAAAFADDKPLRDSDVVFFGADSASDLAGIKKVIPALAINGALWIVYPKG
                     RKEITELQVLNAGREAGLVDIKVVGYSATHTALKFVRPKSKR"
     gene            complement(1967..2089)
                     /locus_tag="VFC15_05545"
     CDS             complement(1967..2089)
                     /locus_tag="VFC15_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09657.1"
                     /translation="MDAKYNWLIDNVTIRVTLNTVAFALWIATAVGLLEVASKL"
     gene            complement(2291..3193)
                     /locus_tag="VFC15_05550"
     CDS             complement(2291..3193)
                     /locus_tag="VFC15_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZM09658.1"
                     /translation="MTSASQIYVGTAGWSYKDWEGIVYPAQLKKSQHPVEYMAQYFDM
                     LEINTSFYGHVKPEWGKLWCRKAGRVNPQFMFTAKLNRAFTHSPIAIAESTSADTIRV
                     GADDELLAKAGLDSIAEENMLGAVLVQFPISFKNTNQNRDYLDALLEKFRQYPLVIEV
                     RHNSWTNEGTLRYFAQKGVAFCNIDQPLLGKAVGPSQLVTSPIGYVRLHGRNYQQWFD
                     SDSGSDRYNYLYNNEELQAWKKRVDSIAEKTRKTFVVTNNHFEGKAAVNALQLKSMLT
                     GAPVKIPSTLRSRYWELGEIESDE"
     gene            3377..3643
                     /locus_tag="VFC15_05555"
     CDS             3377..3643
                     /locus_tag="VFC15_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09659.1"
                     /translation="MIDIKKKCWREKVTAEITVTHVLEFAQEMGANLNAAEVATFLNQ
                     NGTAPNVWIHMMQAGEQYIKSNLKTKALDLPVVNRPGAHAATVQ"
BASE COUNT          792 a          964 c         1106 g          801 t
ORIGIN      
        1 ttgcaactga gacggtggtg ctgttgtcgc cgcaggggca gttgttctcg caagcggtgg
       61 cgcaggaatt ggtcagccgc gaacgcgtgg tcctgatctg cgggcgctac gaaggcgtgg
      121 atgaacgggt gagcgagtac ctggcagacc gcgagatttc agtgggcgat tttgttctga
      181 gcggcggcga gttgggtgca gcgatcattg tggataccat gacgcgattg actcccggag
      241 cgctggggaa tgcggcgtcg gcacagcagg agtcgttcac gccgacggtg gtggacgcgg
      301 gggagagagc ggataacgcg cctccttcgt caacctgcgg gtcgggcgga ttgctggact
      361 atccgcatta cacgcgtccg gcggagtttc gcgggatgtc ggttcccgag gtgctgatga
      421 acgggaacca cgatgaaata aggcgctggc gacgtcgccg tgcgctggag aaaacgttgg
      481 cctaccgtcc tgatttattg cttggggcgg agttgagcaa ggaagaccgc aagctgctcg
      541 aagaaattga aggacgggag tagttgctcc cacgctgtga ttgttctggg ggcgagaaaa
      601 gacccactca agccgaagcg gcttgaatgg ggcacccgag agagagttta agattcaggc
      661 cgccgcgagg cgtgcgacag agaaggaaga aaaacgaaat gtcgaattca ccgattattg
      721 accgattgat tgccaaaacg cggcgcaccg atcttcccga attcaaggcg ggcgacacgg
      781 tccgcgtgca cgtgaagatt aaagaaggcg agaaggaacg cgtgcaggta tttgaaggcc
      841 tggtcatcgc caagagcaag ggcacacagc cgagtttcat cgtccgcaag atgagcttcg
      901 gacagggcgt cgagcgtatc ttcccgttga actcgaaggt catcgagaag atcgaagtgc
      961 tgcgctcggc gcatgttcgc cgcgccaagc tctactacct gcgcgcattg cgaggcaagg
     1021 ctgcccgcct gcgtgaagtc gatgttcgca ccaagaaggc ttagccaggc gctcgcaatt
     1081 acggcgcccc gcctgtcgtg cctgcgggcg caatgggcgt cttctttacg gtgtacgtct
     1141 gttggcgcag ccagctacct gtgagccgct atcgcttaga ttttggccgg acgaatttca
     1201 gtgcggtatg cgtggccgag tagccgacaa ccttgatgtc aaccagtccg gcttcgcgtc
     1261 cggcatttag aacctgcagt tcggtgatct cttttcgtcc tttgggatag acgatccaca
     1321 gagctccgtt gatcgccagc gccgggatca ccttcttaat gcccgccaag tcagacgcgg
     1381 aatcagcacc aaagaagacc acgtcggaat ctctaagggg tttgtcatcg gcgaacgcgg
     1441 cggcagcttt gcgcgcatcc ttcagtacct cggtttcaga caagcgaagc gcagaaatcc
     1501 tcgacccggg tttgatgcca agtttctctg cagtcgactt ggggtggaga atcttgtgcg
     1561 cccatttttc cgcctgctcc cccaattcaa agacagttga gttctccagc cacttgagat
     1621 gaagctcacc gtttcgcgcg accacggact tgatgcttgc gagcgggatc ttcaggcgga
     1681 gatcgccgcg gaacacgatt tcattggttt ccaacagcgc tttgccgtcg gaacgctttc
     1741 caccggagcg caccgaacac tgcaactctt tacccatcga gcctcccaag cattgggaga
     1801 atcgcgcaca tcatggccga atgtcaactc ggtaacttag gaaattcgag gacgatgagt
     1861 acaaaagaaa ccccgctcga cggagatgaa gagcggggct tctttacgtg gcgtggtttc
     1921 gcgcacgcat actgatccca ggggagggcg ttcttgcgat ccgcggttac agcttggaag
     1981 caacttcgag taacccaacc gctgtggcaa tccagagcgc aaacgccacc gtgttaaggg
     2041 tgacgcggat cgtaacgttg tcgatcagcc agttgtattt cgcgtccatt gtaagtctcc
     2101 ttcgtgagtg gtttgcgtcc ggctcgcctt gggctaccgg cccagcttct atggacatat
     2161 acgggctaca ggcgaaaagg ttccgaccca tgtgtgtgag acattgcgag aagcaggaaa
     2221 tgtggatgag cggctggaag ttgctgacga atggcggatg tactacggtg tgggactgtt
     2281 ttgtaacaat ctattcatct gattcgatct ctcccagttc ccaatagcgg ctgcgcaggg
     2341 tcgacggaat cttaaccggg gcgccggtca gcatgctctt gagttgcagc gcattgacag
     2401 ccgccttacc ttcgaagtgg ttgttggtga cgacgaaagt ctttcgcgtc ttttcggcga
     2461 tggaatccac acgcttcttc catgcttgca attcctcgtt gttataaaga tagttataac
     2521 gatcgctgcc ggagtcgctg tcgaaccact gctggtagtt tcggccatgc agtcgcacgt
     2581 agccgatcgg cgatgtcacc agctggctgg ggccgacggc ttttcccagg agcggctgat
     2641 cgatgttgca gaaggcgacg cctttctgcg cgaagtagcg caacgtacct tcgttggtcc
     2701 agctattgtg gcggacttcg atcaccagag gatactgacg gaacttttcg agcaaagcgt
     2761 cgagataatc gcggttttga tttgtgttct tgaaagagat ggggaactgc accagcacag
     2821 cgcccagcat gttttcttcg gcgatggagt ccagccccgc tttggcgaga agttcatcgt
     2881 cagcgcccac gcgaatcgta tctgccgacg tgctttcagc tattgcgata ggcgagtggg
     2941 tgaacgcgcg attgagtttg gcggtgaaca tgaattgtgg gttcacccgg cccgctttgc
     3001 ggcaccacaa cttgccccat tccggcttga catggccata gaacgacgta ttgatctcga
     3061 gcatgtcgaa gtactgtgcc atgtattcga ccgggtgctg cgacttcttg agctgcgccg
     3121 ggtacacgat accttcccag tccttgtagg accatcccgc cgttccgacg taaatttgcg
     3181 atgccgaagt catcaatgca ggcagggtag cacagaccct tacggatcgc tgtcggcctg
     3241 cgactggcgg ttagccgacc cgtagagacc gattgtgact atcgtcacac gatagcgtga
     3301 taccgcacat aggccgcaca tcttgccgga tatattcctg ttcctgcaaa caaatccatt
     3361 ctgtcgggaa gcagaaatga ttgacatcaa gaaaaaatgc tggcgtgaaa aggtgaccgc
     3421 ggaaatcacc gtgactcacg ttctggagtt tgcccaggaa atgggggcca acttgaacgc
     3481 ggcggaagtc gccacgttcc tcaaccaaaa cgggaccgcc ccgaatgtgt ggattcatat
     3541 gatgcaggcg ggcgaacagt acattaagtc gaacctgaaa acgaaggcgc tggatttgcc
     3601 tgtcgtcaat cggcccggcg cacatgccgc gacggttcag tagcaagttg gcgagaaacg
     3661 atg
//
LOCUS       DASRKK010000258         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_409779, whole genome shotgun sequence.
ACCESSION   DASRKK010000258 DASRKK010000000
VERSION     DASRKK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409779"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFC15_05560"
     CDS             complement(<1..419)
                     /locus_tag="VFC15_05560"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM09660.1"
                     /translation="MSSIGTWMQIVAQGWLIYRLSHSAFLLALDQFLGGIPIFLFSLI
                     GGVVADRIERRKVLLASQYVQMASATILTVLVATGLTHVWPILCLSFMSGLAQAFGGP
                     AYSALIPTLVDREDMPNAIALNSIQFNLAVTIGPALAG"
     gene            complement(866..2860)
                     /locus_tag="VFC15_05565"
     CDS             complement(866..2860)
                     /locus_tag="VFC15_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09661.1"
                     /translation="MRDSFRSSVVCLLAIACLLVPVAVCAADSSATTTADPTNAATSA
                     APTNSGANVGAGAVINPTLVDLLVKKGILTTSEANSLRTMSGSAGMQQLLLLLKAKGI
                     VNDSEAAELKSAADADTLHSLVDTESGPTQYASLTTPGSGQAAAPKEAAGPVVIPAIA
                     PVRVLSLDPPVKDGLAPALKLGAVRMTPYGFIKATAAYDTLDPTGDDFPRPGFTNADT
                     GPNKNPEFHLKARATRFGSKFEWPDISKNITVTGQVEADFEGNFSRADNRNVSSIRSN
                     AFQLRLAFGRIDWTVAPNTDIFFEAGQDWTIFGSSALMNLFETTFYGAYWGNLYERSP
                     QMRLGFVQKLGGSLNWKLSPEFAVMMPSEGNLPADAVTCTVAKLGVATTCTVVDGTAN
                     QLGYGERQGADSSRPELEARTVLQFQLDKAPGVVPAQILWSGFYSTRQATVLGSAVPA
                     PYQADFPRGVDNSSHGYGNQIAISLPTRWATVVASGYMGADLRFFFGGETLSNYNQAA
                     GLTETASGFSVDRSSTVVFGLNAAGHPVVAPQKSVRGYGGFIQAGFPISRWFNADPKG
                     RNAGWQAYIEYGLDAANANDFRYAKDIGAAGGGPIKDTIKAFTVFYKMNPWVQFGFEE
                     SQYSGYALPNTSGLCTTKVAGLPTCTSVDWRSEFGPVFTF"
BASE COUNT          726 a          946 c         1036 g          701 t
ORIGIN      
        1 ccggcgagcg caggaccaat cgttaccgct aggttgaact gaatcgaatt cagcgcaatg
       61 gcgttcggca tgtcctcccg gtctaccaac gtgggaatca gcgccgagta cgctggaccg
      121 ccaaaagcct gcgccaagcc ggacataaac gacagacaga ggatgggcca gacgtgggtc
      181 agtccggtgg ctaccagaac ggtgaggatc gtcgcgctcg ccatctgcac gtactgggag
      241 gcgagcagga ccttgcgtcg ctcaatccgg tcggccacca ccccgccgat cagcgagaag
      301 aggaagatgg gaatgccgcc gagaaactga tcgagcgcga gcaagaaggc ggaatggctc
      361 aggcgataga tgagccagcc ctgcgccacg atctgcatcc aggtgccgat gctagacata
      421 caggcgccga accacatcaa acggaaatca cgatagtgaa atgctttgaa gaccctgtgc
      481 aaggttgacg tttgcatgcg ctccgcgtgt ctctgggact ctatcttcgc acgggcacac
      541 gttccggccc ttcaatgacc ggtcagtcac tccagcgatt tggttggcac gggtgtggat
      601 gtggctgaac ccggtcccgc agcagttgcg cccgagttcg cctgttaccg tgtcgccttc
      661 tccagaaggt acatgtacga acggtagatc tgtcccgtgg agcggctcat gccgatttcg
      721 caggtacggc tgcccctgcg gctctggagc aggcgctggc tatggtagcg gaaggtgtca
      781 cttgcgtttg tgaccaagga caccagtcgc aaaaacggga tggcacgctg ctgcggccac
      841 cccgttcagg ttatacaacc gtttcttaga acgtaaacac cggtccgaac tccgaacgcc
      901 aatcgactga tgtgcaagtt ggcaacccag cgaccttggt ggtgcaaaga ccgctggtat
      961 tcggaagggc gtagccactg tattggctct cttcaaagcc aaactggacc caggggttca
     1021 tcttatagaa cacggtgaag gctttgatcg tgtccttgat cgggccgcca ccggccgcgc
     1081 caatgtcctt ggcgtacctg aagtcattgg cattagccgc atccagaccg tattcgatgt
     1141 acgcctgcca gcccgcattc cggcccttcg ggtcggcatt gaaccagcgc gagattggga
     1201 aaccagcctg gataaatcca ccgtagcctc gtaccgactt ctgcggagca acaacggggt
     1261 gcccggcggc gttaagccca aagaccacgg tcgaagagcg atccaccgag aagccgcttg
     1321 ccgtctcggt cagcccagcc gcctggttgt agttggacaa ggtctcgcca ccaaagaaga
     1381 accgcaggtc agctcccatg taaccgctag ctacgacagt tgcccagcgg gttgggagtg
     1441 aaattgcgat ttgattgcca tacccatggc tgctgttgtc aacgcctctg gggaaatcag
     1501 cttggtaagg tgcgggtacg gctgagccca gcacagtcgc ctggcgcgta ctgtagaatc
     1561 cgctccaaag gatctgcgcc ggaactacac cgggagcttt gtcaagttgg aattgcaata
     1621 cggttctggc ttcgagctcc ggacgagacg agtccgcgcc ctggcgctcg ccgtaaccta
     1681 gctggttggc tgtgccgtcg acgaccgtgc aggtcgtggc gacacccagc ttggcgacgg
     1741 tacaggtaac ggcatctgcc gggaggttac cctcggacgg catcatgacc gcaaactcgg
     1801 gggagagctt ccagttaagc gagccgccca gtttctgcac aaagcccaaa cgcatctgtg
     1861 gagagcgctc gtaaaggttg ccccagtagg cgccatagaa cgtagtctcg aataggttca
     1921 tgagcgcgct cgagccgaat attgtccagt cttgtccggc ttcgaagaag atgtccgtgt
     1981 tgggcgccac ggtccagtcg attcgtccga aggccagacg gagttggaac gcattgctcc
     2041 gaatgctgga gacgttgcgg ttgtcagcac ggctgaagtt gccctcaaaa tcagcctcga
     2101 cttgtccggt gaccgtaatg tttttcgaga tatctggcca ctcgaacttg gaaccgaaac
     2161 gagtggcacg cgccttgaga tggaattccg gattcttatt cgggcctgta tcggcgtttg
     2221 taaagcccgg tctgggaaaa tcatcgcccg taggatcaag tgtgtcgtac gccgcggtgg
     2281 ccttgatgaa gccataaggc gtcatcctga ccgcgcctag cttcagggcg ggcgccaggc
     2341 catccttaac cggcggatcc agcgaaagta cccggaccgg cgcgatagcg ggaatgacta
     2401 ccggacctgc ggcctctttc ggagctgcgg cttggcccga gcccggagtc gtgagacttg
     2461 catactgagt gggacccgat tctgtatcga ccagcgagtg caaggtatct gcgtccgcgg
     2521 cgcttttcag ctccgctgcc tccgaatcat tgacaatacc tttggccttc agcaaaagca
     2581 gcaactgctg catccctgcg gaacctgaca tcgttctcag gctgttggct tccgatgtgg
     2641 tcaggattcc cttttttacc aggagatcga ccaaggttgg attgataacg gctcccgctc
     2701 ctacattggc gccgctgttc gtgggtgcgg cgctggtagc cgcgttggtg ggatccgccg
     2761 tggtcgtggc acttgagtca gcagcacata ccgccactgg aaccaagaga cacgcgatgg
     2821 cgagcagaca cactaccgat gaccggaaag aatctcgcat cttcgcctcc aagcaagtgg
     2881 gcgcaattca ataccagacg ggaagcctgg cgtcatcggg attcctgatc ggtaaatccc
     2941 atctctgaat tggccttgga ggggaactgt aagggacagc atggggaagg ctggcggaca
     3001 tggctcacgc gtgaaggtga cataggtcac actgtgtctg tgagtaagga gatatgttgc
     3061 gcctgatgtg cgactcataa gtggcagact ctgcgctggt tagtggaaag aacaagccga
     3121 cgacgaagac gctggagacc cgcgttgcca cttttgtggg agaacttgtc accgctcgcg
     3181 tcggaagcca aggggcaata tcggtttcag gtgtggaagc gcgatcgcac cggtatgttt
     3241 ctttgctgga atcagctacc gatgaagccc tagtactaca gttgtagata agtgtgctac
     3301 tctgaatccg ctctgcggtt tgattctccg gcatgagtga tggagaaacg tctggcggga
     3361 tggaagcgcg agttgttgcg ggcgcatggg ctgatcgcgg agttatttg
//
LOCUS       DASRKK010000259         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1289292, whole genome shotgun sequence.
ACCESSION   DASRKK010000259 DASRKK010000000
VERSION     DASRKK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289292"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFC15_05570"
     CDS             complement(<1..375)
                     /locus_tag="VFC15_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09662.1"
                     /translation="MKTLYSALLASTVAICCLTVQANANTVTIASQNATDFVVNPTDH
                     AQVTLVGLSGTVFNNITGSNSGIDRSPFENDATPGSGVGTWASNRYDAIQANSSGYVN
                     VALSNTMTMLWGSPDTYNTISFC"
     gene            817..1874
                     /locus_tag="VFC15_05575"
     CDS             join(817..1348,1348..1874)
                     /locus_tag="VFC15_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611416.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM09663.1"
                     /translation="MAVLLRGDFDAARLRGLAKKTKDGPQARRLLALATIYEGGTRTK
                     AAKIGGVGLQIIRDWVLRFNARGPDGLLNGKAPGQPSKLNDSQRQSIARMIENGPIPA
                     IHSVVRWRLIDLAQWIFEEFRITIAKQTLSRELRAMGYRKLSARPRHHAQAEGVMEDF
                     KKASPRAWRQSRVRRASNASRIEIWFQDEARIGQKNKITRRWAKRGTRPSAPRDQRTA
                     STYIFGAVCPKQGKGAALILPACNTEAMNLHLAEIAKIVAPGAHAVLLVDQAGWHMSM
                     RLAVPHNITIIALPPKSPELNPVENVWQFMRDNWLSNRVFTSYDDLVDHCCDAWNKLT
                     DQPWRIMSIGLRHWAHGF"
BASE COUNT          442 a          589 c          565 g          420 t
ORIGIN      
        1 gcagaaagat atggtgttgt aggtatccgg tgaaccccac aacatggtca ttgtattgga
       61 gagcgcaacg ttaacgtatc ccgacgaatt cgcttgtatc gcatcgtacc gattggaggc
      121 ccaagtgcca accccactgc cgggcgtggc atcattttcg aagggcgacc gatcaatgcc
      181 tgagtttgag cccgttatgt tattaaacac agtgcctgac agcccaacaa gcgtaacttg
      241 cgcgtggtcc gtaggattca ccacaaaatc agtagcattc tggctggcaa tcgtgacggt
      301 attggcgttt gcctggaccg tcaaacaaca gattgcaacg gtactcgcta aaagtgcact
      361 atacagcgtt ttcatcgcac aaccccctaa ctcacacatg cagacgccac acagttaaga
      421 aagcatgaaa ggtaatttga gtcaataggc gaccggatag gcggtcgttc ataagtctat
      481 gatttaacag ccgtatttta tccaaaaatt tgagcacgct ttatcaatgg catttttttc
      541 gtgtgctagg cgtttttgcc agctttaatt gcagattttg ggtggcgtga ggccgttagc
      601 ctccgaacag gccaaattgt tcgcccgccg actgcttcgg gcatggcgcg accctttggc
      661 tcccgatggt agacgtgaag gaggggaggc cgttccttcc cagaaatacc gcgtcgaccg
      721 tgagcagtaa tacccaatcc ctgagaaact gactcctttc cgattcccaa atcagcgcgg
      781 ctctgattca tatgtggcga acgatggagg ttcgcgatgg cggtattgct tcggggagat
      841 ttcgatgcgg cgcggctgcg ggggttggcg aagaagacca aagatggacc tcaggcccgg
      901 cggctgttgg cgcttgccac gatctacgag ggtgggacgc gcaccaaggc ggcgaagatc
      961 ggcggtgttg ggcttcagat catccgggac tgggtgttgc gcttcaacgc tcgcgggccc
     1021 gacgggctgc tgaacggcaa ggcgccgggg cagccctcca aactcaatga ctcccagcgc
     1081 cagtcgatcg cccggatgat cgagaacggc ccaattccgg cgatccatag cgtggtgcgc
     1141 tggcggctga tcgatctcgc acaatggata ttcgaggagt tccgtatcac gatcgccaag
     1201 cagacgctca gccgcgaact gcgcgctatg ggctatcgca agctgtcggc ccgcccgcgt
     1261 catcatgccc aagccgaggg cgtgatggag gattttaaaa aagcttcccc acgcgcctgg
     1321 aggcaatcac gcgtgagaag agcctcgatg catccaggat agaaatctgg ttccaggacg
     1381 aggctcgtat tggccagaag aacaagatca cccgccggtg ggcaaagcgc ggcacccgcc
     1441 caagcgctcc gcgcgatcag cgcaccgcct cgacctatat cttcggtgcc gtctgcccga
     1501 aacagggcaa gggcgcagcc ctgatcctgc ccgcctgcaa taccgaggcg atgaacctgc
     1561 atctcgccga gatcgccaag atcgtcgcgc ccggcgcaca cgccgtgctc ttggtcgatc
     1621 aggccggctg gcacatgtcg atgcgcctcg ccgtgccgca caacatcacc atcatcgccc
     1681 tgccgccgaa atcccccgag ctcaatcctg tggaaaacgt ttggcagttc atgcgcgaca
     1741 actggctctc caatcgcgtc ttcacatcct acgacgacct cgtcgaccat tgctgcgatg
     1801 cttggaacaa gctcaccgat cagccctgga ggatcatgtc catcgggctc cgccattggg
     1861 cgcatgggtt ctgatcattg agacttggta taactcgtaa ggaaaccaaa tgctcgatgc
     1921 cagccaattg ttttatacgc agatcgccgc cggcgtttgc ctgctgatct tcgccggcct
     1981 cgcctacaat ctgctgcgcc tgcgccgcca gatgga
//
LOCUS       DASRKK010000260         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1387314, whole genome shotgun sequence.
ACCESSION   DASRKK010000260 DASRKK010000000
VERSION     DASRKK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387314"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFC15_05580"
     CDS             <1..561
                     /locus_tag="VFC15_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09664.1"
                     /translation="RNGGCESLTVRLTNPAVLASFDKDIRGNPQVQVKADSERKYYED
                     LAGSVATALIMLASFVAIVMGIGAVFGAMNTMYAIVSQRTREIGTLRALGFSRFSIMF
                     SFVMESVLLALVGGLLGCLIALPMNGYTAATGTNAFSELAFAFKVTPGLLLIGLSFAV
                     LMGFVGGLLPAFRASRMPITRALREA"
     gene            complement(868..1533)
                     /gene="npdG"
                     /locus_tag="VFC15_05585"
     CDS             complement(868..1533)
                     /gene="npdG"
                     /locus_tag="VFC15_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZM09665.1"
                     /translation="MIAIVGGTGPEGSGLALRWVRAGESVIIGSRDAERAKATAAQIA
                     EKVGPMGSVEGAENATAVKMSDIVVLTVPFAGQAELLKQLKPSFRQGTVFIDATVPLA
                     AAVGGRPTRVLGVWQGSAAEQAAEIAGKNVAVAAAFHSLSAAVLQGDQPVDCDVIVCS
                     DDDRARQVASELATKIPGVRAIDGGKLENARIVEAMTALLITLNIRHKVHGAGWRVTG
                     LGI"
     gene            complement(1530..>2901)
                     /gene="cofH"
                     /locus_tag="VFC15_05590"
     CDS             complement(1530..>2901)
                     /gene="cofH"
                     /locus_tag="VFC15_05590"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZM09666.1"
                     /translation="SPGLANASRSNPMAEPRFVEAIGVLRLGLKSSLRMTSLKVVGRD
                     NTWGTMHDISWANRQILQNATWDDVQQRVGAPVRTALDKVLSSLDGSSLTREECLTLA
                     NCSGDDLFGLVVAANDLRRQLVGDVVSYVVTRNINFTNVCFVGCKFCAFSVGPRDETA
                     YFLSLDEVAQKCSEAEQWGATEVCIQGGLPRNLPPFYYRDILRAVKSATPKMHAHAFS
                     PMEMVYGVELTRMPLREYLLMLKENGLDTLPGTAAEILDDSVRHVLSANKLSTAQWCE
                     VIETAHQCRIHSTSTMMYGHTETHEHWVNQLLLLRQIQSRTGGFTEFVPLGFIHDNTL
                     LFGQGLARAGATLDEHLKVHALARVMLAGSINHVQVSWVKLGREVSQLTLLAGADDYG
                     GTLFEENISRLAGATAGQYISTAEFQERILELGRVPAQRNTLYTKYNYPAQSATAEPV
                     AVGGKA"
BASE COUNT          533 a          939 c          836 g          593 t
ORIGIN      
        1 cgcaatggcg gatgcgagtc gctgaccgtg cggcttacca acccggccgt gctcgcgtcc
       61 ttcgacaaag acatccgggg caatccgcag gtccaggtga aggcggactc ggagcgaaag
      121 tattacgagg accttgctgg aagtgtggcc acggcactga tcatgctggc ttcgttcgtc
      181 gctatcgtga tgggcatcgg cgcggtgttt ggcgcgatga ataccatgta tgccatcgtg
      241 tcgcagcgca cccgcgagat cggcacgctg cgcgccctgg gcttctcgcg cttcagcatc
      301 atgttttcct tcgtgatgga gtcggtgctg ctcgccctcg tcggcggact gctgggctgc
      361 ctgatagcgt tgccgatgaa tggctacacc gccgcaaccg gaaccaatgc cttcagcgaa
      421 ctcgcgttcg ccttcaaggt caccccaggg ttgctgctga tcggccttag cttcgcggtg
      481 ctgatgggat tcgttggcgg attgctgccg gccttccgcg cctcaagaat gccgatcacc
      541 agggcactgc gcgaagcgta ggaagcaagg gccaggggct agtttcaagt ttcaagtttc
      601 aagtttcaag tttccagttt cgagcacgaa cttccacgtt gtcatcctga gcgacgaggt
      661 caccgtggcg accgaggagt cgaaggaccc ctataaacgc aagagttcca cggctgtcgc
      721 acgtcgcttc ctcttcgctc tgtcgtcccg attcgctttt cggctttgtc atcccgagcg
      781 gagccgaggg gcctgtggtt tcggaggtgg tcatcctaag gtccaaagcg cgccacaaaa
      841 ctgacaactg gcaactggca actgtctcta gattcccagc cctgtcactc tccatcccgc
      901 gccatgcacc ttgtgccgga tgttgagcgt gatgagcagc gcggtcatgg cttccacgat
      961 gcgcgcgttc tccagcttgc cgccatcgat ggcacgcacg ccgggaatct tggtcgccag
     1021 ctccgacgca acctgccgcg cgcgatcgtc gtcactgcac acaattacgt cacaatccac
     1081 cggttgatcg ccctgcagca ccgctgctga gaggctgtga aacgctgccg ccacggcgac
     1141 gttctttcct gcgatttccg ctgcctgctc ggctgccgaa ccttgccaca cgcccagcac
     1201 gcgcgtgggt cgtccgccaa cagccgcggc aagcggaacc gtcgcgtcga tgaacactgt
     1261 gccctgccgg aacgacggct tgagttgctt cagcaactcc gcttgtccgg cgaatggcac
     1321 cgtgagtacc acaatgtcag acatcttaac tgcggtggcg ttctccgcgc cttccacgct
     1381 gcccataggt ccgaccttct ccgcgatctg ggctgctgtg gccttggcgc gttccgcatc
     1441 gcgcgagccg atgatcaccg actcgccggc gcgcacccat cgcagcgcca gccccgaccc
     1501 ttctggaccc gtgcctccga cgatggcgat catgccttgc cacctaccgc caccggttcc
     1561 gcagtggccg attgcgccgg atagttgtat ttggtgtaaa gcgtgttgcg ctgcgcgggc
     1621 actcggccca gttcgagaat ccgctcctga aattccgccg tcgagatgta ctgccccgct
     1681 gtggcacccg ccagccgcga gatgttctct tcgaacaacg taccaccgta atcgtcggcg
     1741 ccggccagca acgtgagttg cgaaacctcg cgaccgagct tcacccacga aacttgaacg
     1801 tggttgatag agccagccag cattacgcgg gccagcgcat gcaccttcag gtgctcatcg
     1861 agcgtcgcgc ccgcgcgagc caaaccctgc ccaaacaaca acgtgttgtc atggatgaat
     1921 cccagcggca cgaactcggt gaacccgccg gtgcgcgact ggatctggcg caacagcaac
     1981 aactggttga cccagtgttc atgggtctcc gtgtgaccgt acatcatggt cgaggtgctg
     2041 tggatacggc attggtgcgc ggtctcgatc acttcgcacc attgcgccgt gctcagcttg
     2101 ttggcggaga gtacgtgacg cacgctgtcg tcgaggattt ccgccgcggt gcccggcaag
     2161 gtgtcgagcc cgttctcctt cagcatcagg agatactcgc gcagcggcat gcgcgtgagt
     2221 tcgacgccat aaaccatttc catgggcgag aacgcatgcg catgcatctt cggcgtggcc
     2281 gacttcaccg cgcgcaggat gtcgcggtag tagaacggag gcagattgcg cggcagcccg
     2341 ccctggatgc agacctccgt cgccccccat tgttcggctt cgctacactt ttgcgcgacc
     2401 tcgtcgagcg aaagaaaata ggcagtctcg tcccgcggac ctacgctgaa ggcacaaaac
     2461 ttgcatccca caaaacagac gttggtgaaa ttgatgttgc gggtaacgac gtagctgacc
     2521 acgtcgccca ccaactgccg ccgcagatcg ttggccgcaa ccaccaaccc aaacagatca
     2581 tcgccgctgc aattagcgag ggtgagacat tcctcgcgcg tgagcgagct gccatcgagc
     2641 gacgacagca ctttgtctaa ggctgttcgc acgggcgcgc cgacgcgttg ctgcacgtcg
     2701 tcccacgtcg cgttctgaag tatctgccga ttggcccaac tgatgtcgtg catagttccc
     2761 caagtgttgt cccgaccgac gacctttaag cttgtcatcc tgagcgagga cttcagtccg
     2821 agtcgaagga cccctatcgc ttccacgaat ctcggctcag ccatagggtt gcttcgactc
     2881 gcgttcgcta accccggcga a
//
LOCUS       DASRKK010000261         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_898270, whole genome shotgun sequence.
ACCESSION   DASRKK010000261 DASRKK010000000
VERSION     DASRKK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898270"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFC15_05595"
     CDS             complement(<1..778)
                     /locus_tag="VFC15_05595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZM09667.1"
                     /translation="MQDSRSVTVHPARNILGSVAMPGDKSISHRYAMLAAVAAGRSRF
                     QNFSPGADCASTLGSVRALGCHVERDEQGTVVIDGLGPQLRAPDAPLDCGNSGSTMRM
                     LSGIVAGQPFATELIGDESLSRRPMRRVIEPLTRMGAQIHSTESGCAPLRITGARLHG
                     IEYLVPVASAQVKTSLLFAGLLAEGHTTVTEPVRTRDHGELALRACGAKVDRLRNTVT
                     IEGGQALHPLEAYIPGDISSAAFFLCAAAVFPESNLVIDGV"
     gene            complement(781..1188)
                     /locus_tag="VFC15_05600"
     CDS             complement(781..1188)
                     /locus_tag="VFC15_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09668.1"
                     /translation="MLDAFLVPEKTAVTAKGDSEALDISAASNPVFLVTLSIDEVVEQ
                     ESIEINLFTSADGTTWEAKPVATLPQKFYVGEYPLLVNLTATPNAKFVRVHWDVNRWG
                     RGATTPRFVCGVRIREVTPKALLEAKAEASTRR"
     gene            complement(1327..1644)
                     /locus_tag="VFC15_05605"
     CDS             complement(1327..1644)
                     /locus_tag="VFC15_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09669.1"
                     /translation="MSVIDDLRQLLQDLVTPEFRALSVRVDALEKKVDTLDRKVDTGF
                     IALEKKVDAGFTKADVDLKQAMADTEARAERRHTALIIALQLEERVKRLEQDRIASHE
                     QRT"
     gene            complement(1717..2448)
                     /locus_tag="VFC15_05610"
     CDS             complement(1717..2448)
                     /locus_tag="VFC15_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM09670.1"
                     /translation="MLLHPFPLNRHFWDSVTAQLSTRYRLILPDLRAHGDSELGEGPA
                     TMQKLADDLAGLCRKENIRKAFFVGVSIGGYLLFEFWRRNRDQVAALVLANTRPGAET
                     SESKANRLQLADRVLREGTDGFIEEMLTKLLSQTTRTNRPDIVDAARKMMQSMSAEDV
                     AGVQRGMAERPDSVATLASINVPTLLVAGDEDSIPLSEFELMRQHISGSRLQVISQAG
                     HYAALEKPAEFGALLRTFFDEVLRR"
     gene            complement(2761..3084)
                     /locus_tag="VFC15_05615"
     CDS             complement(2761..3084)
                     /locus_tag="VFC15_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZM09671.1"
                     /translation="MYCNYCGKVIQDDANLCPYCGRRIGAVAGRRKLMRSRAERKIAG
                     VCAGFAEYFDLDVTVVRVVWLIVALFGGGGILAYIIAWIVMPEEPEYVPVAPPATNVQ
                     PYQGS"
     gene            complement(3163..>3634)
                     /locus_tag="VFC15_05620"
     CDS             complement(3163..>3634)
                     /locus_tag="VFC15_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09672.1"
                     /translation="ALFLRREEKYDEALKVVSGMQSDFPRNFLVASEYAHLMNAAGHG
                     PEAIAAYRKVLAGCRSNAYSGCRIEVPAYGLGEALRGQRQYQEAAEAYDLASSHGNDP
                     ELRQRATLAAGEMYDVLQERAAALEKYRTVIAEDSSSGAADLARRYMKQAYKSP"
BASE COUNT          726 a         1112 c         1098 g          698 t
ORIGIN      
        1 gcacgccgtc gatcaccagg ttggactcag ggaagacggc agcggcgcac aggaaaaaag
       61 cggccgagga gatgtctccg ggaatgtagg cttccaacgg atgcagcgcc tggccgcctt
      121 cgatcgtcac cgtgtttcgc aaccgatcaa cctttgcacc gcaggcgcgt agcgcaagct
      181 ctccatgatc gcgcgtgcgg accggctctg ttaccgtcgt atggccctca gcgagcagtc
      241 cggcgaagag caatgaagtc ttgacctggg cactggcgac gggaactaga tactcgatac
      301 cgtgcagccg ggcgccggtg atgcgtagcg gcgcgcagcc gctttcggtc gaatgaatct
      361 gggcgcccat cctagtcaga ggctcgatga ctcggcgcat aggccggcgc gagagcgact
      421 cgtccccaat cagttccgta gcgaacggct ggccggcgac gatgcccgac aacatccgca
      481 tggtcgagcc ggaattgccg caatcgagtg gcgcgtcggg ggcccgcaat tgcggaccga
      541 gtccatcaat caccaccgtt ccctgttcgt ctcgctcgac gtggcatccc agcgcgcgga
      601 cagatccgag agtgctggcg caatcggcgc ccggcgagaa attctgaaac cgcgaacgtc
      661 cggcggctac agcggcaagc atggcgtagc ggtgagagat ggacttgtcg cctggcatgg
      721 cgacacttcc cagaatattg cgcgcgggat gtacggtgac cgatcggctg tcctgcatgg
      781 tcagcgccga gtgctcgcct cagccttggc ttccagcagg gctttcggtg ttacctcgcg
      841 gatgcgcacg ccacagacaa acctgggcgt cgtcgcgccg cgtccccagc gatttacgtc
      901 ccagtgcacg cgaacaaact ttgcgttcgg ggtggcagtc aaattgacca gcagcggata
      961 ttcgccaaca taaaacttct ggggcaacgt cgcgacgggt tttgcttccc aggtcgtgcc
     1021 gtcagcagag gtgaacaggt tgatctcgat cgattcctgc tccaccactt cgtcgatgga
     1081 aagggtcacg agaaaaacgg ggttcgaagc cgcgcttatg tccagcgctt cactgtcacc
     1141 cttagcggtg actgcggtct tttccggtac aagaaaggca tccagcatgg cgaacctatt
     1201 ttaccgaagg gccggattga atagtggtga cagaggtcac aagccctatt gtggcgagag
     1261 cgttcccgct gtcgcgtccg acgatggtat cgacaattac ggggtgtggc ggcagggccg
     1321 gagattctag gtcctttgtt cgtgggaggc gatgcggtcc tgctccagcc gcttcacgcg
     1381 ctcttccaac tgcagagcga tgatcagggc cgtgtgccgg cgctcagccc gggcctcggt
     1441 atcggccatc gcttgtttca gatctacgtc ggccttcgtg aagcctgcat caactttttt
     1501 ctcgagcgcg atgaagccgg tatcgacttt tctgtcgagc gtatcgactt ttttctcgag
     1561 ggcatcgacg cgcacactta gggccctgaa ttccggcgtg accagatcct gcaaaagctg
     1621 gcggaggtca tcaatcaccg acatggtggc ccgattatag cgtccgaaat gtggcgccgc
     1681 aacgccgtgt actcgtgcaa gcggttctgc ggaaggctaa cggcgcagga cttcgtcgaa
     1741 gaaggtccgc agcagggcac cgaactccgc cggcttctcc agggcggcat aatggcccgc
     1801 ctgagatatg acctgcaacc ggctgccgga aatgtgctgc cgcatcagct cgaattcgct
     1861 gagcggaatg ctgtcttcat cgccggcaac gagcagcgtg ggcacgttga tgcttgccag
     1921 cgttgcaacc gaatcggggc gctcggccat tccgcgctgc acgccagcga cgtcctcagc
     1981 agacatgctt tgcatcatct tgcgcgcagc atcgacgatg tccggacggt tcgtgcgggt
     2041 ggtctgcgac aacaacttcg tcagcatttc ttcaatgaag ccgtcggttc cctcgcgcag
     2101 cactctgtcc gcaagttgca accggttcgc tttgctctcg gatgtctcgg ccccaggtcg
     2161 ggtattagcc agcacgagcg ccgccacttg atcgcggttt cggcgccaaa actcgaagag
     2221 cagatagccc ccgatggata cgccgacaaa gaaggccttg cgaatgtttt ccttgcggca
     2281 cagccccgcc aggtcgtcgg cgagcttttg catggtggcc ggaccttcgc cgagttcgga
     2341 gtcgccatgc gcccgcaggt cgggaaggat cagccggtat cgcgtactga gttgtgcagt
     2401 gaccgagtcc cagaagtggc gattcagcgg gaacgggtgc agcagcacga cgtcaggacc
     2461 atcgccgacg acttcgtatg agagctgtgc atcgccgctg aagaaggtgg gcatggtgga
     2521 agaagcaatt agcaattagc aactaacagt tagcaaccag cagtcaacac tcagcattga
     2581 gccgttcaag ccgtctagca tgcttgaaca taggcttgcg gcccacccat gaggatgaaa
     2641 atctaccacg gaggcccgcc gatcgcggag aaaactccaa ctccatagaa tccaaaactc
     2701 cgtgccctcc gtgttctccg tggtgaatgg ttttcgacgg aacgaaatcc gaccgcgtcg
     2761 tcacgatccc tggtacggct ggacgttggt cgcgggcggc gcaaccggga cgtattccgg
     2821 ctcttccggc atcacgatcc acgcaatgat gtaggccagt atgcccccgc ctccgaacag
     2881 agcgacgatc agccaaacca cgcgaaccac ggtcacatcg agatcgaagt actcggcaaa
     2941 gcccgcgcac acgccggcaa tcttgcgttc agcccgcgaa cgcatgagct tgcggcgacc
     3001 ggccaccgcc ccaatgcggc gtccgcagta agggcacaga ttggcgtcgt cctggattac
     3061 ttttccgcaa tagttgcagt acatacagcc tccctacaca ggtaatacgg atagcatgga
     3121 aagaagttcc cgcgtctgca aagccggtgc cgaagcgggc cgtcaagggc tcttgtaggc
     3181 ctgcttcata tagcggcggg ccaagtcggc cgcgccggag ctggaatcct cggcgatcac
     3241 cgtccggtat ttttccagcg cggcagcgcg ttcctgcaga acgtcataca tttcgccggc
     3301 tgcgagggtc gccctctggc gcagctcggg atcgttaccg tggctggagg ccagatcgta
     3361 agcctcggcg gcttcctggt actggcgctg accgcgcagc gcttcgccca atccatacgc
     3421 cggcacctcg atacggcacc ccgaataagc attgctgcga cagcccgcca ggaccttgcg
     3481 ataggccgca attgcctccg gcccatgtcc cgcggcattc atcaggtgcg cgtactcgga
     3541 ggccaccaga aagttgcgcg ggaaatcgga ctgcatgccg ctgacgacct tcagggcctc
     3601 gtcatatttc tcttcccggc gaagaaacag ggcc
//
LOCUS       DASRKK010000262         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1191882, whole genome shotgun sequence.
ACCESSION   DASRKK010000262 DASRKK010000000
VERSION     DASRKK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191882"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFC15_05625"
     CDS             <1..973
                     /locus_tag="VFC15_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09673.1"
                     /translation="DYLTVNDMLAVEQQVPGVQEASPVLNLHEPILVGNGKQRDMLIL
                     GVDPEYVTVRRIVVSSGRFFDRQDSQSHHKVALVTEDFAARQYGGIEFAAGHEIDIQG
                     LPFVVIGAFREGVETFGQSEIQDDTVLIPYSVARYLTDTNAVNQIYFSMSDAGSVPRA
                     SQRILAVLKARHRAESVYNVGDLTQVLGLVAKTANAFTIILLLFATITLVAGGVGIMN
                     IMLATVSARIREIGIRKAVGATRREIQMQFLSEAVMISLIGGTIGTLAGLALPFSLRI
                     FTQYRLPVSALSALIAMLVSCLIGITFGTLPAKNAARLDPVESLRYE"
     gene            complement(1705..>2289)
                     /locus_tag="VFC15_05630"
     CDS             complement(1705..>2289)
                     /locus_tag="VFC15_05630"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09674.1"
                     /translation="HPDTLTARSNLAGTLYAQGDLAGARQQFEQVLEARRRLLGEQHP
                     ATLTARLNLAGTLKAQGDLAGARQQQEQVLEARRRLLGEEHPDTLTARNNLASTLYAQ
                     GDLAGARQHQEQVLEARRRLLGPDHPDTSVSAWWLCATLRNLGEREAARAVLKRDLLW
                     LLDRDPATLGADQRKAREYVAQVVKHYPPEEESG"
BASE COUNT          427 a          676 c          690 g          496 t
ORIGIN      
        1 ggattatcta accgtgaacg acatgttagc tgtggaacag caggtgccag gcgtgcaaga
       61 agcgtcgccg gtgctcaatt tgcatgaacc gatactggtt ggcaacggca agcaacgcga
      121 catgttgatc ctgggtgtgg acccggaata tgtgactgtg cgccgcatcg tcgtgagttc
      181 aggcaggttt ttcgatcgac aagactccca atcccaccac aaggtcgctc tggtcacgga
      241 agactttgcc gcgcggcaat acggcggaat cgagttcgcg gcgggacacg aaatcgacat
      301 ccaaggattg ccgttcgttg tgatcggcgc ctttcgcgag ggcgtggaga cgtttggcca
      361 gtcggagata caggacgaca cggtccttat tccatattcc gtggcgcgtt atttgacgga
      421 tacgaacgcc gtcaaccaga tttatttttc gatgtcggat gccggtagcg ttccccgtgc
      481 ctcgcaacga attctcgcgg tgctaaaggc gcggcatcga gctgaatcgg tttataacgt
      541 tggcgatctc acccaggtgc ttggcctggt ggcgaaaacg gcaaatgcat ttaccatcat
      601 tctcttgctg tttgcaacca tcactctggt tgccggtgga gtagggatca tgaacatcat
      661 gctggccacc gtgagcgccc gtattcgaga aatcggcatc cgtaaagcgg ttggagcgac
      721 gcgccgcgaa atccagatgc aattcctctc tgaagcggtc atgatctccc tcatcggcgg
      781 gaccatcggc accttggccg ggctggcgct cccgttttcc ctccggatct tcacccagta
      841 ccggcttccg gtatcggcgc tttccgcgct gatcgcaatg ctagtctcct gtttgatcgg
      901 cattaccttc ggtaccctgc cggccaagaa tgctgcgaga ttagatcccg tggagtcgtt
      961 aagatacgaa tgagatgaga cgtaagatat tttgagtggc gtgaaggtga ttcgtaaagc
     1021 agtagccagt agtcagtagc cagtagtcag ttgccagtcc taccctttcg ccaaaggccg
     1081 gcgaaaggat ggggcaccct gatatcttat cccgtcccgc gagcgggact tgggcattgc
     1141 gaactcggaa gttgatgttg agactaaggt tgaatctgga acctatctca taaatcgctt
     1201 tgtatcaggg cacgcctttc agccgtgccg cagcccgtta aacaaagaac ttgggcttta
     1261 gagcggtttc agagttagcg tagacctggc cgggtagtgc ggaccttcag gtccgcgtta
     1321 atagcgtttt agaaaaaccg ggctttagcc cttggggtac ttcggcctgg cggccctcgg
     1381 aggcgctttc ggctgattcc aacaccgaac cgcagggcct aaaggccctt ccatccggag
     1441 ccttatgacg cggcgctgaa gcgccgcact acccgagcgt cggtcttgct ccgggttggt
     1501 attgctgcgg aggcgaaact tccgcagttc acgctaactc tgaaactgct ttagcccctg
     1561 atggagcttg tttgcggggg ctgaagcccc ggtcttttct gcgccctgtt cggcacgact
     1621 aaaagcctgc cctgagcgaa gccgaagggt cgtgccctga cacgaaccgt aaagatttgt
     1681 gggacgagct gtagtcgcta tccactaacc actctcttcc tctggcgggt agtgcttgac
     1741 gacctgggcc acgtactcgc gggctttgcg ctggtcggcg ccgagggtgg cgggatcgcg
     1801 gtcgagcagc cacagcaggt cacgcttgag cacggcccgt gccgcctcgc gctctcccag
     1861 gttgcgaagt gtcgcacaca gccaccaggc ggagacggat gtatccgggt ggtcgggccc
     1921 cagcagtcgc cgacgggcct ccagcacctg ttcctggtgc tgccgcgcac ccgccaagtc
     1981 gccctgcgcg tacagcgtac tggccagatt gttacgcgcc gtcagggtgt cgggatgttc
     2041 ctcgcccagc agccgccgac gggcctccag cacctgctcc tgctgctgcc gcgcgcccgc
     2101 caagtcgccc tgcgctttca gcgtaccggc cagattgagc cgcgccgtca gggtggcggg
     2161 atgttgctcg cccagcagcc gccgacgggc ctccagcacc tgctcgaact gctgccgcgc
     2221 gcccgccaag tcgccctgcg cgtacagcgt gcctgccaag ttgctgcgcg ccgtcagggt
     2281 gtcgggatg
//
LOCUS       DASRKK010000263        35369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_19350, whole genome shotgun sequence.
ACCESSION   DASRKK010000263 DASRKK010000000
VERSION     DASRKK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 35369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35369
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19350"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFC15_05635"
     CDS             complement(<1..995)
                     /locus_tag="VFC15_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09675.1"
                     /translation="MYRCLLVLLLSITTAAQTSPAPSATAFDGKSWWNYVKVLADDNM
                     EGRETGSEGLRKAAAYIVEQLKNDGLEPAGSNGYYQPVKLISRQIDESGSSLALVRNG
                     ATEPLTLGEDAMFSMRVDLAPEVDAPLVFVGYGLNVPEKKYNDFAGLDLKGKVAVIIS
                     GSPSDMPAALASHYQSAAERSQALRTAGAIGLISIPNPASMDIPWSRMTLARTRPSMS
                     LAGPNMNESAGIQLAAVFNPADAEKLFQGSGHTFQEIAALAKDRKPIPVFPLTASIRA
                     HAKVIQEESDSANVVAKLTGTDPKLKNEYVVLSAHMDHLGIGEPINGDRIYNGAM"
     gene            complement(1104..2693)
                     /locus_tag="VFC15_05640"
     CDS             complement(1104..2693)
                     /locus_tag="VFC15_05640"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HZM09676.1"
                     /translation="MAIVAGVDFGTLSVRVSLVDSERGVLASAVAEYPLHRKREDPEY
                     ATQSHDDHMRALTKATRDALNDAAVSGDRVEAIALDTTGSSVVPVGKDLKPLDEYYLW
                     CDHRSKGEAAEITDAAHRESLEAINWCGGVYSSEWGFSKLLHWLRHNPEKRSELVTAL
                     EHSDMVAATLAGVTDAKKVKRSACAMGHKWLWNPKLGGLPSEKFLVKVDPLLAGVRER
                     LDGDYATSDQIAGRLSQYWAAQLGLSAGIPIPVGAFDAHWDAIGAGCRTDDMVNVIGT
                     STCIIGITASVNLVPGVCGVVEGSVHPGRTGIEAGLSAVGDIFGAIATRAGTDVNTLS
                     QGLERYRAGQTGLLRMTWDNGDRTVLVNPNLRGITLGWNLQSTAQDELFAAIEGTAFH
                     TRVILDRMSSSGVNIRRVINAGGIPQKNTVLNQVYANVLGRPVLVPTQSVTSLGSAIF
                     AFLAAGTFKTVDQAQDKICPPHKVYEPQAAEKQTYDELYALYSELYFAFGQPAAHDIG
                     SVLPKLMQVAAAVRNSPATVA"
     gene            complement(2807..3505)
                     /gene="araD"
                     /locus_tag="VFC15_05645"
     CDS             complement(2807..3505)
                     /gene="araD"
                     /locus_tag="VFC15_05645"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HZM09677.1"
                     /translation="MLLQTLREEVLDANLELVRRGLVLYTFGNVSGISRADGLIVIKP
                     SGVPYEDMRPEHLVVTDLDGKVVEGTLRPSSDLPTHLVLYQAIPNIGGVAHTHSDYAT
                     AWAQARRPIPCFGTTHADSFHGPVPVTDTLNDDEIAIEYEKNTGRAIVRALGGTEGAH
                     SPGILVANHGPFTWGPDAHTAAQNAAILESIARMAYLTLAIDATAKPIGQGLHDKHFL
                     RKHGANASYGQKKD"
     gene            3717..4112
                     /locus_tag="VFC15_05650"
     CDS             3717..4112
                     /locus_tag="VFC15_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM09678.1"
                     /translation="MRRFDGLYVTLTSRRDRHSHPSSRVRASGLENTCVDEGAYVLIT
                     GRRKSELEAAVKEIGRSVTGVQSDVSKLADIDALYAVVKKQKGKLDIVFANAGTGEFA
                     PLGQITEEHFDKQFDVNVTTLPLSLAQRV"
     gene            4430..5193
                     /locus_tag="VFC15_05655"
     CDS             join(4430..4745,4745..5193)
                     /locus_tag="VFC15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM09679.1"
                     /translation="MEITQAQYRRIEACLPRQRGNVSMTNLSVLNAILYVAEQGCKWR
                     GLPKRFGNWHTIYTRMNRWSKNGTLNRVFEQLQREQIVRIRIESVQLDSTIVKVHPDG
                     TGAFKKNGPQAIGKSRGGWTTKIHMVAADARTAITFALSPGRASDSVQGRELLGGIGP
                     LPAPLHLIMDRAYEDNETRQLALSFGFIPVVPLKGNRLEPWQYDKAIYRKRNEIERLF
                     RRLKGFRRIFSRFDKLDVVFLAFLNFALIVEALRLC"
     gene            5605..6591
                     /locus_tag="VFC15_05660"
     CDS             5605..6591
                     /locus_tag="VFC15_05660"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZM09680.1"
                     /translation="MGKLRQYLRFMTAVQLLPALESGEETLVGGQAVLEGVMMRSPHA
                     WGISVRKPDGTISTHSEPLERLSEKHPWMGWPVVRGIMTLGQAMVLGFRALRYSADVA
                     LDQVPADENGKKAEISGWVAALNIILSVGFFIALYKFVPLVAATKLKNVYPVFGNQIM
                     FNVVDGVIRIAIFLLFIWGVSLMPDIRRVYQYHGAEHKTVFAFESRDPVTIKSAQTYS
                     TFHPRCGTSFLMTVMLISIVVYMLFPVHTFWARFALRVALLPVIAGLSYEMIRFAAKH
                     GSSLFALLTKPGLWLQRITTKQPSDDQVETAITALNEAMELEKVRGGELVIA"
     gene            6743..7822
                     /gene="prfA"
                     /locus_tag="VFC15_05665"
     CDS             6743..7822
                     /gene="prfA"
                     /locus_tag="VFC15_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZM09681.1"
                     /translation="MFERLEQTELKYEELNKALASPDIVSDSQRYQKTAKAHSEIAPI
                     VEKYREYKDLTRGIAESKALVEGETDPEMLAYAHEELTKLDKRAHEVEEELKFLLLPK
                     DPNDEKNVVLEIRAGTGGDEATLFAAEMFRMYNRYAEMNRWKVEVLSSSDSGVGGLKE
                     VIAIIEGQGVYSRLKYESGVHRVQRVPQTETQGRVHTSAVTVAVLPEAEDVDVKIEAK
                     DIRIDTFCSSGPGGQSVNTTYSAVRITHLPTNTVVSCQDEKSQIKNREKGMRVLRARL
                     YEMEQQKQADALAKERKAMVGSGDRSEKIRTYNFPQNRVTDHRVGFTAHQLPEFMDGK
                     LQPMIDALITHFQSEKLKAESTVLA"
     gene            7862..8794
                     /gene="prmC"
                     /locus_tag="VFC15_05670"
     CDS             7862..8794
                     /gene="prmC"
                     /locus_tag="VFC15_05670"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZM09682.1"
                     /translation="MQLKQALTSAVGQLESADVGSPRLNAETLLMFVLGVNRAYLYAH
                     PERELNSEEQARYDEVIAQRSTGMPSQYITGHQEFWGLDFVVSPAVLIPRPETEHLVE
                     TVLELAREAAHSKIVDVGTGSGCIALALASELKTAEVYGVDLSADALEIARANAAHLQ
                     LDQRVRFLRSDVLTSLSENPDLCHPERSESAPADERSRRTPFAGMSFDDFDFVVSNPP
                     YVGKNEADKVQRSVFEFEPRIAVFSGECGLDIIRPLIGQAYRALKPGGWLAMEIGYSM
                     RDMVLELLSPTMWDDVRVVPDLQGIPRVIAAQKR"
     gene            complement(8800..10362)
                     /gene="purH"
                     /locus_tag="VFC15_05675"
     CDS             complement(8800..10362)
                     /gene="purH"
                     /locus_tag="VFC15_05675"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZM09683.1"
                     /translation="MATIHRAILSVTDKTGLADFARQLAGMGVELISTGGTAKLLRDS
                     GIAVKDISELTGFPEMLDGRVKTLHPKVHGGILHIRDNPQHRAAVAEHGIEPIDMVVV
                     NLYGFEKTASKPGVEFHDIIENIDIGGPSMVRSAAKNFQDVAIVTSPADYESIAKEMV
                     AGGGSLSLLTKWRLAQKAFATTAAYDSAIASTLERIAAPETAVFDLSAGEGFPATLRL
                     SFNKALDLRYGENPHQKAALYNDRSGQGVANSKQLQGKELSYNNIVDLQAAWDLAQEF
                     DEPVVAIIKHTNPCGTAIAPTLSEAYKRALECDPVSAFGGVIGVNRPIDAVTATEMAK
                     LFLEVIAAPDFDAAAREIFAAKKNLRLVEVVPMEQKWVLKNVSGGVLLQDNDVRPLTG
                     ADLKVVSERQPAEQEMRDLLFAWKVCKHVKSNAILYARNGQSTGVGAGQMSRVDSARI
                     GAMKSVLGIKGSVAASDAFFPFPDGVEVIAEAGATAIIQPGGSVRDQEVIDTANKLGL
                     AMVLTGVRHFRH"
     gene            complement(10649..10999)
                     /locus_tag="VFC15_05680"
     CDS             complement(10649..10999)
                     /locus_tag="VFC15_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09684.1"
                     /translation="MNIGETIRNYRLQKGMSQGDIEKRTGLLRCYLSRVENGHTIPSL
                     DTLAKIATAMDLPLAQFFHESNGDNGNKSAPLLADDEVRFLTQIRRYSSSLNDSDRKL
                     VIAMVKKMAAPASR"
     gene            11512..13143
                     /locus_tag="VFC15_05685"
     CDS             11512..13143
                     /locus_tag="VFC15_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZM09685.1"
                     /translation="MNPVEPRHAINPWIVTLAVMLATFMEILDTTVVNVAVPHIAGNM
                     GATVEEGTWVVTSYLVSNAIILPMSGWLANRFGRRRILLSCVMGFTVTSLLCGMATSM
                     EALIFFRILQGLTGGGLQPLAQAVLLETFPPKQHGSAMAAFGLGIILAPILGPTLGGW
                     ITDNYSWRWIFYLNLPVGVISVMMISAFVRDPSYIGKHKTGIDLWGIGFLALGFGMLQ
                     VVLDTGQRKDWFSSPQIRLWAALCVFGLVALVVRELMAEHPIVDLRVLKDRTFAAGTF
                     LMTMLGFVLYASLLLLPIFLQTLLGYPALQSGLALSPRGIGSLIMMPITGQLTNRYDP
                     RKLIAVGLIVGGWTMISLSHLNLNAGYWDIFWPQVIQGGALAFLFIPLMATSMSGIAK
                     EKMGNATSIYNLMRNIGGSFGIATMTTFLARRGQVHQNQLIVHVTPYDHGTQMSLRGM
                     QAWFQMHGSNSVDAMKKSMGAIYGMVQQQAALLSFVEAFWIMGVIFFCMLPLLLFLRN
                     ARDLHPHERKSKGGRDGQPTDAKPEPEPELIGAFH"
     gene            13127..14038
                     /gene="gluQRS"
                     /locus_tag="VFC15_05690"
     CDS             13127..14038
                     /gene="gluQRS"
                     /locus_tag="VFC15_05690"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZM09686.1"
                     /translation="MAHSTSDEARSTITPVPAIPATSVTPYRGRVAPSPTGLLHLGHA
                     CTFWTAYQRALDHQGTLIFRNEDLDPQRSQPKYAEAMLEDLRWLGIRWQEGPDVGGPF
                     APYQQSLRRDFYLAAWRKLRDTGAIYPCSCSRKDLALSASAPDDGDDEPMYPGRCREK
                     IDETANYEAPAGVNWRLRVPDGEVIQFVDQCQGPQSYVVGEDFGDFVIWRRDDVPAYQ
                     LAVVVDDAAMQITEVVRGADLLKSTARQLLLIRALTLPTPTYFHCDLVCDEVGMRLAK
                     RHDALSLRALRELGHTPEQVLAMCDRH"
     gene            14105..14530
                     /locus_tag="VFC15_05695"
     CDS             14105..14530
                     /locus_tag="VFC15_05695"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HZM09687.1"
                     /translation="MKVNWSAIVLAAIVHFGFGAVWFTAFSKAWQAGTRIPPEEMQAY
                     MAHPNFWPYIISFLCSLGMALVISWVMGGFCKYNLFRGIVTGLMVGIVAALAMITEMA
                     FEMRSSTFMFISAAYPLIGSVLMGIIIGVWKPKGVLAAS"
     gene            14560..14904
                     /locus_tag="VFC15_05700"
     CDS             14560..14904
                     /locus_tag="VFC15_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09688.1"
                     /translation="MHGHLRVKDGLDRLPGPNGERFVELFNHETLSVELYAPRRTDPQ
                     QPHPRDEVYVIASGTGRFLRGEEEVIFERGDVLFAAAGVFHRFVDFSQDFAAWVFFYG
                     PEGGEKDSHSEK"
     gene            14937..16412
                     /gene="gatB"
                     /locus_tag="VFC15_05705"
     CDS             14937..16412
                     /gene="gatB"
                     /locus_tag="VFC15_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZM09689.1"
                     /translation="MVNTPEAPVAKQPKYEPVIGLEVHVQLLTKTKIFCGCSTQFGNP
                     PNTNVCPVCLGLPGALPVLNRQAVEFAVRASMALNCRMNERSIFARKNYFYPDLPKGY
                     QISQYDKPLAEHGYIEIPTPSGAKRIGITRVHMEEDAGKSLHDGLADSSQWTSIDLNR
                     SGVPLIEIVSEPDISSPDEAWEYLTRLKEIILYTAVSDCNMEEGSLRCDANVSIRPVG
                     QKKFGTKAEVKNVNSFRFIRQALEYEIVRQEQVLESGGSITQETRLYNAGEGKTYSMR
                     SKEQAHDYRYFPEPDLLPLVVDEACRKQIAAQLPELPEDKRKRLVEHYGITEADAATL
                     TRTQSMADQFEEAASAAKNPKRVANLVQAELLGRLKVKGLEIEQSPISMKGIAFSADL
                     VESGAISGKQLKELYDLAFERNQDFPAIYEKEKPQQITDTGAIEKMIDEVIAANPKQL
                     EQYRAGKKTVAGFFVGQIMKASKGQANPALVNELLSKKLDG"
     gene            16461..17555
                     /locus_tag="VFC15_05710"
     CDS             16461..17555
                     /locus_tag="VFC15_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09690.1"
                     /translation="MTQRLSVMAACILVGAALTGCGTPGAPQLPSLQLARPVEDLTAS
                     RKGTKIQLDWTLPRKNIDRTLVKNIPQSLICRHDGTALMSGCTVVATAPNPKPEEKHK
                     HEQPPALHMKYVDTLPAALGEQNPAGFVRYAVEIMNSRERSAGLSNQVLIPVAPTIAP
                     PEQLAVKVEADGVLISWVGATVPTAPTGLTYRYRVMRSPAGANAYIALADIEPASEGF
                     YLDKTFGWETKYDYRITSVTLVHSQTINMSVEGDDSKATEIFTRDIYPPAEPTGLQAV
                     FSSVGQMPFVDLTWAPNMESDLAGYNVFRRLAGGAAVKLNKLLVAVPSFRDDNVVPGK
                     KYVYAVSAVDARGNESPRSAETTEVVPDKQ"
     gene            17566..18369
                     /locus_tag="VFC15_05715"
     CDS             17566..18369
                     /locus_tag="VFC15_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZM09691.1"
                     /translation="MRFCRFESDADSEKVARYGLIDSGGTQDVIRRTLDHLPTGESDF
                     ERGNPVDIPLASVRLLAPSSPSKIVCIGRNYIEHAKELNHPIPTEPLIFLKPPSAVLA
                     PGDTILRPAELSQRVDHEGELGVVIGKRCHALDEGDDVRPFILGYTCVNDVTARDLQN
                     KDGQWSRAKGFDTFCPFGPIVVSGLDPWSGVRVQTRVNGEIRQDGTTADFIFPLDVIL
                     RFISQVMTLEPGDLIATGTPAGVSPLQAGDVVEVTVEGVGSLRNPVGNA"
     gene            18455..18886
                     /locus_tag="VFC15_05720"
     CDS             18455..18886
                     /locus_tag="VFC15_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09692.1"
                     /translation="MSSLGYYPILALGATAAIVGYALLSRKAKPSSDLMEYEHRTELT
                     RGGRIIDGTVIDVHEREETGTQRQMILLVYTYDVAGVSYEAAQDVTHLRQFIDLYSCR
                     LGLPASVKYDPHNPGDSIVISETWSGLRKSPIRARSRPVAS"
     gene            19002..19724
                     /locus_tag="VFC15_05725"
     CDS             19002..19724
                     /locus_tag="VFC15_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZM09693.1"
                     /translation="MHGLPLPLAAFVAGILSFLSPCVLPLVPGYVSLISGATVEDLQA
                     GDRRMLGTVMLHSLTFILGFSVVFIALGAVATGIGQFANQYHNLLAKAAGIVVIVFGL
                     HLTGLLKIKALYADKRLHDVKGGSSALGSFAVGFAFAFGWTPCIGPILATILVFAGAQ
                     ETVFKGVLLLAIYSAGLAVPFLLTSLGVDRFLGFYTRFRRHLLTVEVISGVLLIVVGV
                     LIFMNNLKLLSGYLAFLNRFAL"
     gene            19738..20352
                     /locus_tag="VFC15_05730"
     CDS             19738..20352
                     /locus_tag="VFC15_05730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZM09694.1"
                     /translation="MKRNALVFAAMFIAILAMLATGKYLDRTHRHGAPVLVGDVRGIT
                     APNFELATLDGQRVKLSDYRGKAVLLNFWATWCGPCKVEMPWFVDLQKKYGNDGLVIL
                     GIAMDDSETPKIAQVASEMGVNYPVLLGTDKVSEAYGNVELLPTSFYIDREGKIVGKG
                     EGLLGRNEIEQNVQKALASPVAGMNKAQASPTQAKGVKVPAIAQ"
     gene            20349..20840
                     /locus_tag="VFC15_05735"
     CDS             20349..20840
                     /locus_tag="VFC15_05735"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HZM09695.1"
                     /translation="MSRNLVYLLLAFVVTGVASSQEPGRKVPSVTLAPVGRVAIKAGS
                     TANVELDFRIGSEFHINSNKPHSELLIPTAMKLNAAEPVNVASLKYPAGQDMSFPFAP
                     DEKLSVYSGDFTITAVIKAPPKAANGTYPVTGELRFQACDRSACYPPRLIPVKFEVTV
                     VGR"
     gene            complement(20883..21386)
                     /gene="ispF"
                     /locus_tag="VFC15_05740"
     CDS             complement(20883..21386)
                     /gene="ispF"
                     /locus_tag="VFC15_05740"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZM09696.1"
                     /translation="MSFNTQVERVPRIGFGWDSHEFKAGIPLRIGAVLLPHDSGLAGH
                     SDGDVLLHALTDALLGAVAAGDIGSYFPPSDPQWKGADSAVFLREAMKQIAKKGYRVG
                     NVDCTLILAAPKIGPHAKAIQQSVAQLLGIDPEQVGIKAKTPEGMGTDNAAIAHAVVL
                     LTEIMWP"
     gene            complement(21395..22129)
                     /gene="ispD"
                     /locus_tag="VFC15_05745"
     CDS             complement(21395..22129)
                     /gene="ispD"
                     /locus_tag="VFC15_05745"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZM09697.1"
                     /translation="MKVIVIIPAAGLGTRMSAASAGHKSKQFFELQGTPILIHTLRKF
                     ANHSQVDEIVVALRKNETAGFLQQVEQEGFKKPIRVVEGGEHRQDSVANALAALNAAP
                     DDIVLVHDAVRPFVDDETIRGVIEAVKKYDSAIAGVPAVDTVKQVDRTADGAVVLATV
                     PRERMVMAQTPQGFRFGLLKKAFDDASADGFLGTDEASLIERAGVTVHVVMGSPRNLK
                     ITTPGDLELAEFFLSQEVRRKTIPTR"
     gene            complement(22228..23316)
                     /locus_tag="VFC15_05750"
     CDS             complement(22228..23316)
                     /locus_tag="VFC15_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM09698.1"
                     /translation="MDLVVIRVLFVFITALSSYELRPLGLERWPAAGVGLLFGLAVVL
                     FEIRLRNVSLKRLIGAVIGSILGIVGAYLFSLVIRNSLQPGPTQSFLQLFLMLLMAYI
                     GLVVGAGKGDLLNLAALGGIFGGEKQGKKSYKILDTSVIIDGRIADIAETGFLDGVIV
                     LPQFVLRELQLVADSADSMKRNRGRRGLDILQRIQKIASLTVQIVEDDFPAVREVDLK
                     LIELAKVYEGKIVTNDFNLNKVAQLQGVAVLNINELANSLKPIVLPGEVMKVFILKEG
                     KEYNQGVAYLDDGTMVVVDNARKMIGKNIDISVTSVLQTTAGKMIFGKFDERAPMMPP
                     RPMEPRPELRKPVPVPGPAVVPDIPKAE"
     gene            complement(23664..24413)
                     /locus_tag="VFC15_05755"
     CDS             complement(23664..24413)
                     /locus_tag="VFC15_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZM09699.1"
                     /translation="MTDTIAAVPAEYKPLTEMERVVDTFVAPSKTFTDIRRNASWWVP
                     WLVMSIFGLAMVFVVDKKLGMDTAYENQLRLSPKQMDKYEQAPPDQKATQLRIGGTIT
                     RYFAYGSPLLTIIAAAVFAGVLMATFNFGFGAEVKFKQAIAISMYAFLPSILKTLIAT
                     AVVGFGAAESFTFQNQVASNLGGLVDPTTSHFLYAVLVAIDAFTIWTLILTGIGYSCV
                     TRVKRGTCMGVVFGWWVVVTLAGAGFGALFA"
     gene            24682..25614
                     /locus_tag="VFC15_05760"
     CDS             24682..25614
                     /locus_tag="VFC15_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZM09700.1"
                     /translation="MHVHASPATNAATSKVLRISLVVTLVYIVLLVVAGVRAHSLALL
                     SEAGHNLSDFFALLLSWGAVYLQGKSPDARKTFGYNRAGVLAAFINGTSLVLISFYIW
                     YEAFRRLLNPVDVHAQLMIWVAAAGVIMNGGIALLLMRGRRDINLRSAIVHEIGDALS
                     TAAVIAGGVAIAFTGQRWIDPALSIGIGVMILWSSIGIIRESLNILLEGTPSGMELER
                     IESAIRTIPGVNDVHDLHVWSIGSDTHSLSCHVSIADMRASESETLLRQIRDQLGSRF
                     HIHHTTIQFEYAVCEVAHGCVIPSSPGHEHTHDH"
     gene            25637..26080
                     /locus_tag="VFC15_05765"
     CDS             25637..26080
                     /locus_tag="VFC15_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HZM09701.1"
                     /translation="MIRLSHAVGGVALVVVILAAMGCSKKGPSAPATEQPSSAATTDA
                     SPSGWKLDLKVTPDRPRMGRPATFAVHITDSLGKPVENAQLTGSLNMTLMDMGKTAVK
                     FGPKGKGDYEAIVPSFDMSGPWELIVDAAQGTLRARKVFPITVFD"
     gene            26217..27272
                     /gene="hemE"
                     /locus_tag="VFC15_05770"
     CDS             26217..27272
                     /gene="hemE"
                     /locus_tag="VFC15_05770"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZM09702.1"
                     /translation="MSAPESLFVRACKCLPVETTPVWFMRQAGRYMKEYRDVRKQYSL
                     VEICKKPEVAAEVTITAAEYLGVDAAIIFADLLLPLEVMGMPFHFSPGEGPVIEKPIR
                     SAADVKVLRTDRAADLGYVAEAVGKVVKHFGSKLPVIGFCGAPFTLASYMVEGGGSRH
                     YVEVKKLMYNSPEVWEELVNKLVVVLAEYAAEQVRHGADVIQIFDSWVGCLSVEDYRR
                     YALEPTTRLVNQLQKATGVPIIYFGTDSTTLLPSMSETGAEVVGLDWRVPLDEGWATV
                     GHKHAVQGNLDPVTLFASWSEVKQRAEHILRLADKRPGHIFNLGHGILPLTPVENVKN
                     LADFVHEFSSSAKATRK"
     gene            27272..28207
                     /gene="hemH"
                     /locus_tag="VFC15_05775"
     CDS             27272..28207
                     /gene="hemH"
                     /locus_tag="VFC15_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZM09703.1"
                     /translation="MSKMAVLLIAHGSPERVEDIPEFLQNISRGRPMADSVVREVQHR
                     YSLIGSSPLTRITRQQAQNVAGELGIPVYVGMRNWHPFIADTLKQMASDNVGQAVAIC
                     LAPHNSRTSVGAYKESLFGENAAFPIDFVEEWHDHPLLIEAFAEKLRAGWAKACEEHG
                     SRLPVLFTAHSVPMRTITEGDPYEAQTRETAALVAKQVPEIGEWKFAFQSQGMSGGEW
                     LGPTVEDTITSLQSEGCTGIFLQPIGFVCDHVEILFDIDILFRQFAAERGMKLYRAES
                     LNDSPTFAKAVADLVRTRLNAGPPCAGSLAAEKMQ"
     gene            28214..29641
                     /gene="hemG"
                     /locus_tag="VFC15_05780"
     CDS             28214..29641
                     /gene="hemG"
                     /locus_tag="VFC15_05780"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZM09704.1"
                     /translation="MKRVAIIGGGISGLSAAFYLEKARAAGADITYTLFESGHRLGGS
                     MYSDRVDGCLVEAGPDSFLTEKPWALALCKELGIDDQLIGSNDAQRKTYIVVKGKLVV
                     MPDGLMFMVPTKLVPTALSPLFSWGTKLRMARELMHPPRPMETDETVGELVERHFGAE
                     VVDRLADPLLSGVYGGDANKLSARAVLPRFVEMEEKYGSLSRAMLAAHNKMRAMAGKQ
                     PPRPLFSSLKDGMQQLVDAIVARLDPAAIRMRTRVRGVYPENDGWRVAIDMGGSENFD
                     AIIVATPANVAGALLEAVDRELADDLLGITYSSSVTVTLGYHMDQLKHLPPGFGFLVP
                     RSEGTRMLACTFVHNKFPHRAPADKGILRCFLGGARDEAVLNLTDDDIVDTVRAELKN
                     IVKLDARPMFDRVYRWRGAMAQYESGHIARVERIEKRVKEIPGLALAGNAFHGIGVPD
                     CIREGMNAANAIVQVAGVTAIPQAR"
     gene            complement(29666..30217)
                     /locus_tag="VFC15_05785"
     CDS             complement(29666..30217)
                     /locus_tag="VFC15_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09705.1"
                     /translation="MIPESHSLQALFTELIHHHFDRDIGLRDSEVQDYVVNVLTEFCE
                     VEQMFKLHNAKERPLDDVGEMLLEADPIYGPAPSFDRERQVRKHIGDYTLFLSGMFPE
                     SINHFRLRRQRLENFVDFIKAGKESYFIVSKFDQFEYARLAPLFGKLAHEFESCVYGL
                     NLVKGDLDEMQHPITRRATEIVM"
     gene            30373..30942
                     /locus_tag="VFC15_05790"
     CDS             30373..30942
                     /locus_tag="VFC15_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZM09706.1"
                     /translation="MNQYENHAQLRLIYRRCRSALVVLLAERLVCCQNASAKRECLKF
                     PLVAQFWIREEAVMQRQNISGNSPYEPKVGFSRAVKVGPFVSVAGTVATGADGKIVGG
                     KNMYAQAVQTLKNIERALAQAGASFSDVVRTRTYVTDVSRWEEAGRAHGEVFGDIRTA
                     ATMIQVVAMVSPEMLIEIEADAIIAAELT"
     gene            complement(31007..31303)
                     /locus_tag="VFC15_05795"
     CDS             complement(31007..31303)
                     /locus_tag="VFC15_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZM09707.1"
                     /translation="MAAGMTKTQLVRHIAEKHSLSNKQVAELIEYLADVAIRETKKNG
                     VFVIPGLGRLVKSARKARMGRNPQTGEAIKIAAKTVVKFRVAKAVKDAIAPPKK"
     gene            31654..32430
                     /locus_tag="VFC15_05800"
     CDS             31654..32430
                     /locus_tag="VFC15_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZM09708.1"
                     /translation="MHISHATILFLAGLAAGAINSVAGGGSFVTFPALLFVGIPPVNA
                     NASNAVAVWPGQAVSIGAYRSQFRNLAWSTMTPLIITAVLGGILGGWVLLKTPQTIFM
                     HLVPWLILAATLIFAMSGRVTRWVRQRTASGEHHEFSNVRAVSIQIFIAFYIGYFGAG
                     AGILILAMLALLGMDEIHTMNALKALLTTVSNGVAMVLFVVAGAVYWPETIVMGVGSI
                     LGGYFGAYFAQKTKPEYVRAIVIGIGFTLSIYYFARQILS"
     gene            complement(32427..32738)
                     /locus_tag="VFC15_05805"
     CDS             complement(32427..32738)
                     /locus_tag="VFC15_05805"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZM09709.1"
                     /translation="MNFVPIVGFGAGTLCTLAYLPQALHSFRTKSVRDISLIMLLSLN
                     VGLVLWVAYGFLIRSLPLILPNAITFFLAFPLLIMKIRYRHMTTVPAKSEAGEDSRAS
                     L"
     gene            complement(32784..33269)
                     /locus_tag="VFC15_05810"
     CDS             complement(32784..33269)
                     /locus_tag="VFC15_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZM09710.1"
                     /translation="MDAKPWLMLILFLFICLGAAGLASFLTARGVREWYPSLRKPPGT
                     PPGFVFGPVWTVLYILMATSAWLVWRGYGWRGGGSALVLFFGQLALNMAWSGIFFGAR
                     LPGVAFAEILILWLAILFNIVVFHWLTQVAALLLVPYLLWVTYASYLNFGIWWLNRGR
                     A"
     gene            complement(33332..34312)
                     /locus_tag="VFC15_05815"
     CDS             complement(33332..34312)
                     /locus_tag="VFC15_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZM09711.1"
                     /translation="MKAVVVTRLGGPEVLEVRELPDPIANSGEVIVRVEAVGVNFADT
                     LSTRGAYKSTPPPPFISGREFAGIVEGTQERVMGYAQYGAAAEKVAVSRKLIWPQPKG
                     WTSQQSAAFPVNFFTAYLAYWKAGITSDAIEPVYVEGERRRRVLIHAVGGGVGTAAVQ
                     IGKLLRVETYGTASTTEKLDRVQKLGLDHGINYREVDYETRISELTNGEGVDAVFEML
                     GGEHTAKSLRCCRQFGRVIIYGTATGERQKFDVGAMMARSLSAHGLWLSVLANDHALM
                     NDCLKAMQPWIEQGKLHPEIGHTLPMEEAGEAHRLLLQRANYGKTVLTIG"
     gene            complement(34379..35233)
                     /gene="accD"
                     /locus_tag="VFC15_05820"
     CDS             complement(34379..35233)
                     /gene="accD"
                     /locus_tag="VFC15_05820"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZM09712.1"
                     /translation="MAWFKREQGELDTSGEKRVKTEGLWVKCDDCRQIIWKKDLDQNF
                     HVCPKCDRHFRIDARTRLAYLLDDGKYETEDGNLISNDPLKFTDLKSYADRLKRAKSD
                     TGLNDAIINARGKLNGKPVIVSAMEYAFIGGSMGAVVGETLTRAIERATAEKIPLIIV
                     SASGGARMMEGVVSLMQLAKISAALARMDDAKVPYISVLTDPTTGGTTASFAMLGDLN
                     IAEPGALIGFAGPRVIEQTNRQKLPEGFQRSEFLLQHGMLDAVVHRRELKNYIFRALD
                     FMQVPATS"
BASE COUNT         7196 a        10365 c        10327 g         7481 t
ORIGIN      
        1 tccatggcgc cgttgtaaat ccggtcgcca tttatgggtt cgccgatgcc gagatggtcc
       61 atgtgggccg acagcacgac atactcattc ttcagcttgg ggtcggtgcc cgtcagcttg
      121 gccacgacgt tggccgagtc actttcctcc tggatgacct tggcgtgcgc tctgatgctg
      181 gcggtcagcg gaaagacggg aatcggcttg cgatcttttg ccagagccgc aatctcttga
      241 aaggtgtggc cggaaccttg gaacagcttc tccgcgtcgg caggattgaa gacggcggca
      301 agctggatac ccgccgactc gttcatgttg gggccggcga gggacatgct gggacgtgtt
      361 cgcgccagtg tcatacggct ccacggaatg tccatcgatg cgggattggg gatcgagatc
      421 agaccgatag cgccggccgt tcgcaacgcc tgtgaacgtt cggccgcaga ttgatagtgc
      481 gaggccaagg ccgcaggcat atccgacggg gagccagaga tgattacggc caccttgccc
      541 ttgagatcga gtccggcgaa gtcgttgtac tttttctcgg gaacattcag gccatagccg
      601 acgaaaacca gcggcgcgtc aacttcgggc gccagatcga cgcgcatact gaacatcgcg
      661 tcctcgccca gtgtcaacgg ttccgtcgcg ccattacgga cgagggcaag gctggaaccg
      721 gattcgtcaa tctgccggct gatgagtttc actggctggt aataaccgtt gctaccggcg
      781 ggttccagtc cgtcgttctt tagttgttcg acgatgtagg ccgcagcctt gcgcagacct
      841 tcgcttccgg tttcgcggcc ttccatgttg tcgtcggcca gaaccttgac gtaattccac
      901 cagctcttgc cgtcgaaggc ggtagcggag ggcgcggggg aggtttgggc agcggttgtg
      961 atcgagagca gcaggaccag caagcagcgg tacatgaaaa atcctcggaa atcgaattcg
     1021 tcattgtcgc ataacgacca tcacctggat ttccgtttgt ggatttaaac ttcgcgcaca
     1081 ccgaagtctg cgacattcgc cgctcacgca accgttgcgg gagaattacg cacggcggca
     1141 gccacttgca tcagttttgg caaaactgat ccgatgtcgt gcgccgccgg ttgcccgaag
     1201 gcgaaatata gctcgctgta taaggcgtac agttcgtcgt aggtttgctt ttccgcagcc
     1261 tgcggttcgt agactttgtg aggcgggcaa attttgtcct gggcctggtc gacggtcttg
     1321 aatgttccgg ccgccaggaa cgcgaagatc gcagagccca agctggtgac gctctgggtg
     1381 ggaaccagca ccggccgccc cagcacgttc gcatacacct ggttcagcac cgtgttcttt
     1441 tgcggaatgc cgccggcatt gatcacgcgc ctgatgttta caccgctgct agacatacga
     1501 tcaaggatta cgcgcgtgtg aaaggctgta ccttcgatgg cggcgaacaa ctcatcctgc
     1561 gccgtgctct gcaaattcca gcccagagtg atgccgcgaa ggttggggtt caccagaacc
     1621 gttcgatcgc cgttgtccca ggtcatgcgc agtaatcccg tttgtccggc acgataacgc
     1681 tccaacccct ggctcaacgt gttcacgtca gttccggcgc gggtggcgat cgctccgaag
     1741 atgtcaccca ccgccgataa tccagcttcg atgccagtgc gtcccggatg tacgctgccc
     1801 tctacaactc cgcacacgcc gggcaccagg ttcaccgacg ccgtgatgcc gatgatgcag
     1861 gtcgaggtcc caatcacatt gaccatgtca tcggtgcggc aacccgcgcc tatggcgtcc
     1921 cagtgcgcgt cgaacgcccc caccgggatg gggatgcctg cgctcaaccc cagttgtgcg
     1981 gcccagtact gggaaagccg tccggcaatc tggtccgacg tggcgtagtc tccgtcaagc
     2041 ctttcgcgta ctccggccag caagggatcg actttgacca ggaatttctc agagggcaag
     2101 cccccgagtt tgggattcca cagccacttg tgccccatcg cacatgcact gcgctttacc
     2161 ttcttcgcat cggtcacacc agcgagcgtg gccgccacca tgtcggagtg ttccagcgcg
     2221 gttacgagtt cagagcgctt ctcgggattg tgtcgcagcc agtgcagcaa cttggaaaaa
     2281 ccccactccg aggagtaaac gccgccgcac cagttgatgg cctcgaggct ttcacgatgt
     2341 gcagcgtcgg taatctctgc cgcctcgccc ttggagcgat ggtcgcacca caggtagtat
     2401 tcgtcgagcg gcttcaagtc cttgccgaca ggaaccaccg acgagcctgt cgtatcaagc
     2461 gcgatcgcct cgactcgatc gccggacact gcagcgtcgt tcagtgcgtc gcgcgttgct
     2521 ttggtcagcg cgcgcatgtg atcgtcgtgc gattgtgtcg cgtactctgg gtcctcgcgt
     2581 ttgcgatgca acgggtattc cgcgacggcc gacgccagca cgcctcgttc gctatcgacc
     2641 agggacaccc ggacactcaa cgttccaaaa tcgacgcccg ccacgatagc catggtctct
     2701 ccgttgtctt acttgtttgt tggtcgagca aaccaccaca ccacataacg tggtgaatgt
     2761 ggaactcatg aagccacacg atggccagat gcgccgtcat tcagatttag tccttcttct
     2821 gaccgtagga ggcgttcgca ccgtgttttc tcaagaaatg cttgtcatgg aggccctggc
     2881 cgattggttt cgcggtcgca tcaatcgcaa gggtgaggta agccatccgg gcaatcgact
     2941 ccagaatcgc cgcattttgg gctgccgtgt gcgcgtccgg tccccaggtg aaagggccgt
     3001 gattggccac taggatgccc ggtgagtgcg caccctcggt gcctcccagc gcccgaacga
     3061 tggcacgacc ggtattcttc tcatactcga tggcgatttc gtcgtcgttc agagtgtcgg
     3121 ttacgggaac cgggccgtgg aaggagtccg catgggtcgt accaaagcag ggaattgggc
     3181 ggcgcgcctg tgcccaagct gttgcgtagt cagagtgcgt gtgagccaca ccgccgatgt
     3241 ttgggatggc ctggtacaga acaaggtgag tcggcaggtc ggaggaaggc cgcagagttc
     3301 cttcaaccac ttttccatcc aggtcggtta cgaccagatg ctcgggacgc atatcctcgt
     3361 acggaacccc gctaggtttg attacgatca gaccgtctgc ccgggagatt ccgctgacgt
     3421 tgccgaaggt gtagagaacc agccctcgtc gcactagctc caggttggca tccaagactt
     3481 cttcgcgaag agtttgtagc agcataaagt tactaacttg cgacgccaag caatcgttta
     3541 cttgactgca ccaacctaga cgaccgattg tatctggtgc cgggaggggg acttgaatcc
     3601 ccacaaccct ttcgggtctg cggattttaa gagcgaccaa cacacatcat aaatactata
     3661 aatcaatgat gtacaagcac ttaactaaaa gtgatggttt ggtcttgcaa tgaaaaatgc
     3721 ggcgcttcga cggtctgtac gtcacgttaa cgtcacgtcg cgatcgtcat tctcacccct
     3781 cttcaagagt gcgtgcttcg ggtcttgaga acacgtgcgt cgatgaaggc gcgtacgttt
     3841 taatcactgg acgccggaag agcgaactcg aagccgcggt gaaagagata gggcgtagtg
     3901 tgacgggtgt tcaaagcgat gtctcaaaac tggcagacat cgacgcgttg tatgcggtgg
     3961 tcaaaaaaca gaaaggtaag ctcgacattg tttttgctaa tgccggtacc ggcgagtttg
     4021 ctccgcttgg ccaaattacc gaggagcatt ttgataaaca gttcgacgtg aatgtgacca
     4081 ccctgccgtt atctctggca caaagagtgt gattgataaa accaggttgt acttggcgac
     4141 ggcaccgcga gagtcctgcc atacctcttc gcggatgaga ccttttccga gagcacaccg
     4201 cacttgggtc gtttaatcca cgaccttgcg tagcccccgg gtcttttaag accgaccctc
     4261 ccaaatcgga agatacttta aataaacgat atgaaagcac ttagctaaaa tagactgctt
     4321 ggttttgccg tgcaaaatgc ggctttttga cgttgtgtac atcacgttta cgtcacggcg
     4381 aagtagggcg tgttaacact aactgatatt tccgtggtat tctgtttttg tggagatcac
     4441 gcaagcgcag taccgtcgca tagaggcctg tttgccgcgt cagcgtggca atgtcagcat
     4501 gacgaacctg tcggtgttga acgcgattct gtatgtggcc gagcagggat gcaagtggcg
     4561 tgggctgccc aagcgcttcg gcaattggca cacgatctat acccgcatga accgctggtc
     4621 gaagaatggc acactgaacc gcgtctttga acagttgcag cgggagcaga tcgtgcgcat
     4681 ccggatcgaa tccgtgcaat tggactcgac catcgtgaag gtacatcccg acggcaccgg
     4741 cgcgttaaaa aaaacggacc gcaagccatc ggcaaatccc ggggcggatg gaccaccaag
     4801 attcatatgg ttgccgcaga tgctcgaacg gccataacct tcgccctctc gcccggccga
     4861 gcctccgact cggtccaggg ccgcgagctt ctgggtggca tcgggccgct accggcgccc
     4921 ctgcatctca tcatggatcg ggcttacgag gacaacgaaa cccggcaact ggctctgagc
     4981 tttggcttca tcccggttgt gccactcaag ggcaatcggc tggaaccatg gcagtacgac
     5041 aaagccattt accgcaaacg caacgaaatc gaacggcttt tccggaggct aaaaggcttt
     5101 cgccgcatct tctcgcgctt cgacaaactg gacgtcgtct tcctcgcatt cctcaacttc
     5161 gccctgatcg tcgaggcctt gcgtttgtgt taacacgccc tggtcacgct tttgcgaagg
     5221 tgatgcttca ctgagcttat cggcgggaac ctgcagaact gtagccatgg atttcgcctc
     5281 tccggcgtca ctacaacacc ccgttgattt ggtcgggaag cagctccgcc ctgacagaat
     5341 cgcggctaag tcccacgggc tgaaatggat agcgataact actagtagaa ttaacgagat
     5401 gagagcaatt taagactaag tcgatgaaaa caaagggagc gtgataaagc aaagtcggca
     5461 aaattgagca tagtggctct tcgagaccaa gctgacgaat ccgccctcga tcaggcactt
     5521 cccggtttga caccccctcc gaacctgcta cccttgatac tgccgatgtc tgcgtcgttg
     5581 gcttctgagg aacatccaat cgttatggga aaattgcgcc aataccttcg tttcatgact
     5641 gctgtccaac tcctgcctgc tctggaaagc ggcgaggaga ccctggtcgg cggccaggcc
     5701 gtgctggagg gcgtcatgat gcgctcgccg catgcttggg gtatctccgt gcgcaagccc
     5761 gatggcacga tttcgaccca ctccgagccg ttggagcgcc tgtcggagaa gcatccctgg
     5821 atgggctggc cggtggttcg cggcatcatg acgctggggc aggccatggt gctgggcttc
     5881 cgcgcgctgc gctattcggc cgatgtggcg ctggaccagg ttccggcgga cgagaatggc
     5941 aagaaggccg agattagtgg atgggtcgcg gcactcaaca ttattctgtc ggtgggattt
     6001 ttcatcgcgc tgtacaagtt cgttccgctg gtggcggcca ccaagctgaa gaatgtctat
     6061 cccgtgttcg gcaaccagat catgtttaac gtggtcgatg gcgtgatccg tatcgcgatt
     6121 ttcctgctgt ttatctgggg cgtgtcgctg atgccggaca tccggcgggt gtaccagtat
     6181 cacggggctg agcacaagac ggtgttcgcg ttcgagtcgc gcgatccggt gacgatcaag
     6241 agcgcgcaga cgtactcgac ctttcatccg cgctgcggaa ccagcttcct catgacggtg
     6301 atgctgattt cgattgtggt gtacatgctg ttcccggtgc atacgttctg ggcgcggttc
     6361 gcgctgaggg tggcgctgct gccggtgatc gcggggctgt cgtacgagat gattcgcttc
     6421 gccgccaaac atggctcgtc gctgtttgcg ctgctgacca agccgggctt gtggctgcaa
     6481 cgcatcacga ccaagcagcc atcagacgat caggtggaaa ccgccatcac cgcgctcaac
     6541 gaagcaatgg agctggaaaa agttcgcggt ggcgaactgg tgattgccta ggcggcgccc
     6601 gccgctacga aacgctctcc caatattgaa tgtgcggaac ggctggcctg aaggccagcg
     6661 ctaccacgtt ccgttttcct gttcccgtaa aatgaattgt ttgctggcgc gcaggtttgc
     6721 gaagcggccg gagtaggcat ccatgtttga acgattagaa caaaccgaat tgaagtatga
     6781 agagctgaac aaagctctgg cgtctccgga tatcgtctcc gactcgcagc gctatcagaa
     6841 gacggcgaag gcgcatagcg agatcgctcc catcgtagag aagtatcgcg agtacaagga
     6901 cctgacgcgc gggatcgcgg agagcaaggc gctggtcgag ggcgagaccg atcccgaaat
     6961 gctcgcctat gcgcatgaag agctcacaaa gctggataag cgcgcacatg aggtcgagga
     7021 agagctgaaa tttttgctgt tgcccaagga tcccaacgac gagaagaacg tggtgctgga
     7081 aatccgcgcg ggcactggtg gggacgaggc gacgctgttc gcggcggaga tgttccgcat
     7141 gtacaaccgc tatgccgaaa tgaatcgctg gaaggtggaa gtgctgtcga gttcggactc
     7201 gggcgtcggc gggctgaagg aagtgatcgc catcatcgaa ggccagggcg tgtattcgcg
     7261 gctgaagtac gagagcggcg tgcatcgcgt gcagcgcgtc ccgcagaccg aaacccaggg
     7321 ccgtgtgcat acttcggcgg tgacggtggc cgtcctgcca gaagcggaag acgtggacgt
     7381 gaaaattgag gcgaaagaca ttcgcatcga tacgttctgc tcgtcgggcc cgggcggaca
     7441 gtcggtgaat accacgtatt cggcggtgcg cattacgcac ctgccgacga atacggtggt
     7501 gagttgccag gacgagaagt cgcagatcaa gaaccgcgaa aagggaatgc gcgtactgcg
     7561 cgcccgtctc tatgaaatgg aacagcagaa gcaggcagat gcgctggcca aggaacgcaa
     7621 ggccatggtg ggttcgggcg atcgcagcga gaagatccgg acttacaact ttccgcagaa
     7681 ccgcgtcacc gaccaccggg tcggcttcac cgcacaccag ttacccgagt tcatggacgg
     7741 caagctgcag cccatgatcg acgcgctaat tacgcacttc cagtcagaga agctcaaggc
     7801 ggagtcgacg gtgctggcct aagacttcgc ctgacggggt tgcagccgct agaatctctc
     7861 cgtgcaactc aagcaagcgc tcacgtctgc ggtagggcaa ctggagtctg ctgacgtggg
     7921 ctcgccccgc ctcaacgccg agacgctgct gatgtttgtg ctcggcgtta atcgcgccta
     7981 tctctacgct catcctgagc gcgagttgaa ttccgaagaa caagcccggt acgacgaagt
     8041 catcgcgcaa cgatcgaccg gcatgccttc gcagtacatc accggccatc aggagttttg
     8101 ggggttggat ttcgtggtgt cgccggcggt actgattccg cggccggaaa cagagcacct
     8161 ggtcgagacc gtgcttgaac tggcccgtga agccgcacac tcgaaaatcg ttgacgtggg
     8221 aaccggctca ggctgcattg ccttggcgct ggcgagtgag ttgaagactg cggaagtgta
     8281 cggggtcgat ctctccgccg atgcgctgga gatcgcccgt gctaacgcag cgcacctgca
     8341 attggaccag cgggtaagat tcttgcgcag cgatgtactt acctcgcttt ccgaaaatcc
     8401 tgacctgtgt catcctgagc gaagtgagtc cgcgccagcg gacgaacgca gtcgaaggac
     8461 ccctttcgcc ggaatgagtt ttgacgattt cgactttgtg gtcagcaacc cgccttacgt
     8521 cgggaaaaac gaggccgaca aggtgcagcg atcggtgttc gagttcgagc ccaggatcgc
     8581 ggtgttttca ggcgaatgcg gcttggatat cattcgcccg ctgatcgggc aggcgtacag
     8641 ggcgttgaag cccggcggct ggctggcgat ggaaattggc tactcgatgc gagacatggt
     8701 gttggaactg cttagtccca caatgtggga tgacgttcgg gtcgtgccgg acttgcaagg
     8761 gattccaagg gttatcgccg cacagaagcg ataacgccct cagtgccgga agtgccgcac
     8821 gccggtgagc accatggcta gtcccagctt gtttgccgtg tcgatcactt cctggtcgcg
     8881 caccgagccg cccggctgga tgatcgccgt cgcgccagct tcggcaatga cttccacacc
     8941 atccgggaat gggaagaacg cgtcggaagc ggcgacggag cccttgattc ccagcaccga
     9001 cttcatggcg ccaatgcggg cggagtccac gcggctcatt tgtccggcgc cgactccggt
     9061 gctctgtcca ttacgcgcat aaagtatggc gttggacttg acgtgcttgc agaccttcca
     9121 ggcaaacagc aggtcgcgca tctcctgctc ggcaggttgg cgttcgctaa caaccttcag
     9181 atcggcgcca gtcaaggggc gcacatcgtt gtcctgcagt aacacgccgc cggagacgtt
     9241 tttcaatacc cacttctgct ccatgggaac gacttcgacg aggcgcaggt tcttcttggc
     9301 ggcgaagatt tcacgcgcgg ctgcgtcgaa atcgggcgcg gcaattactt cgaggaacag
     9361 cttcgccatt tcagttgcgg tgacggcgtc gatggggcga ttgacgccga tgacccctcc
     9421 gaacgctgag acgggatcgc actccagcgc gcgtttatac gcctctgaca atgtgggagc
     9481 tatggcggtg ccgcaagggt tggtgtgctt aatgatggca acgacgggct cgtcgaattc
     9541 ctgcgccagg tcccacgcag cctgcaaatc gacgatgttg ttgtagctga gttccttgcc
     9601 ttggagttgc ttgctgttgg ctacaccttg gcccgatctg tcgttataga gcgccgcttt
     9661 ctggtgcggg ttctcgccgt agcgcaaatc taaggccttg ttgaatgaca ggcgcagggt
     9721 tgcgggaaag ccctcacctg cactgagatc aaacactgca gtctcaggcg cggcaatgcg
     9781 ttctagggtc gaggcgattg ccgagtcgta agccgcggtg gtggcgaatg ccttctgcgc
     9841 cagccgccac ttggttagga gcgagagcga tccgccacca gccaccatct ccttcgcaat
     9901 cgattcgtag tcagccggcg aagtcacaat ggcgacgtcc tgaaagtttt tcgccgcgga
     9961 gcgcaccatc gacgggccgc cgatgtcaat gttttcgatg atgtcgtgga actcgacacc
    10021 cggcttggag gccgtttttt cgaaaccata gagattgacc acgaccatgt cgatcggctc
    10081 gattccgtgt tcggcgacgg cagcgcgatg ttgcgggtta tcgcggatgt gaagaatgcc
    10141 gccgtgcacc ttgggatgca gagttttcac cctaccgtcg agcatctcgg ggaatccagt
    10201 gagctcagag atgtccttta cggcgatgcc ggagtcacgc agcaactttg cggttccacc
    10261 cgtggagatc aactccactc ccataccagc gagttgacgc gcgaagtcag ccagaccggt
    10321 tttgtcggtg acactgagaa tcgccctatg aattgttgcc atgattgcct cgtaaagatt
    10381 aagaatgctg cggcagccgc attcgactaa aagccgaagg ggccgactca tcagaatagc
    10441 ggaagcggcg cattccgagg aatgattggc acgatttcag acaagggaga tccaggcggg
    10501 attgaaaata gcaggaagaa cagattcccc gcgggctaat gcccgcgacc aatgacaaaa
    10561 attaaagggc attcacggtg caaaagaggc tgcgcccctt ccatacatca ttgcggggat
    10621 ggtggcgcgg ttgcgcgtgc aacgcgagct agcggctggc gggcgccgcc atcttcttca
    10681 ccattgcgat cacgagtttg cggtcactat cgttcaggct cgacgaatag cggcgaatct
    10741 gggtgagaaa tcgcacttcg tcatcggcca gcaggggcgc gctcttatta ccgttgtcgc
    10801 cgttactttc atggaagaat tgtgcgaggg gaagatccat tgcggtcgcg atcttggcca
    10861 gcgtgtcgag ggaagggatg gtgtgtccgt tctccacgcg cgacagatag cagcgcaata
    10921 gtcccgtgcg cttttcgatg tcaccctgtg acatgccttt ttgcaaacgg tagttgcgga
    10981 tagtctcgcc gatattcatg ccagaatggt ttccttagaa aaagtggtct gaagttgact
    11041 gcatggtaac ggcttgtatc tgcggaagca agcaaaactt atatttccaa gcgatttctt
    11101 tctcggctgc aaaggcaatt gagtagtaag tggttaccta tttatcacta aagtgatgga
    11161 gtctcatgcg acccatgtgg cggacaggtc acgtgaactg gctgggattt acgttctact
    11221 catcagtctg taataaaccg gcggaggcga acgcaaatcc acctgagtat cgctgaacca
    11281 gtcttcaatc tatttctcgg ggctaatgag tccgggtgag aacctgggtc gccgctacgc
    11341 cgctcgggtc aatttcctac tttacccggg cttgcgcccc gggctcactg catcgccgcc
    11401 gctgcgcggc tgatctgctg caggtttcca accaaccaat ctgcgagcaa tgtgtcgcgc
    11461 gtcacttcct ttccctctct gggtttttat aatcacgggt cggactttgt gatgaacccc
    11521 gtcgaacccc ggcacgcgat taatccgtgg atcgtcaccc tcgccgtgat gctggcgacg
    11581 ttcatggaaa tcctcgacac cacggtggta aacgtcgcgg tcccgcacat cgcgggcaac
    11641 atgggcgcga ccgtcgaaga gggcacatgg gtcgtgacct cgtacctggt ctccaacgcc
    11701 atcatcctcc ccatgtcagg atggctcgcc aatcgcttcg gccgccgccg cattctgctc
    11761 tcctgtgtca tgggattcac cgtcacctcg ctgctgtgcg gcatggccac cagcatggag
    11821 gcgctgatct tcttccgcat tctccagggc ctcaccggag gcgggctgca acctctggcg
    11881 caggcggtct tgctggaaac attccctccc aaacaacatg gctcagccat ggccgcattc
    11941 ggattgggca tcatcctcgc gcccattctc ggccccaccc tcggcggttg gatcaccgat
    12001 aactattcct ggcgctggat tttttatctc aaccttcccg tcggtgtgat ctcggtgatg
    12061 atgatcagcg ctttcgtgcg cgacccgtcg tacatcggca aacataagac cggcattgat
    12121 ctctggggca tcggttttct cgccctcggc tttggcatgt tgcaggttgt gcttgacacc
    12181 ggacagcgga aagactggtt cagcagtccg caaatccggc tctgggcagc gttgtgcgtc
    12241 ttcggcctgg tcgcgctcgt ggtgcgcgag ttgatggccg agcatcccat cgtggatctg
    12301 cgcgtcctca aggaccggac attcgccgcc ggcaccttcc tcatgacgat gcttggcttt
    12361 gtgctttacg ccagcctgct gctgctgccg atcttcctgc aaaccttgct tggatatccc
    12421 gcgctgcaat ccggactggc gctgtcgccg cgtggcatcg gatcgctgat catgatgccc
    12481 attaccggac aactcaccaa tcgctacgac ccgcgcaagc tcattgcagt cggcctgatc
    12541 gtggggggct ggaccatgat cagcctgtcg cacttgaacc tcaacgcagg ctactgggac
    12601 atcttctggc cgcaggtaat ccagggcggc gcactggcat tccttttcat tccgctgatg
    12661 gccaccagca tgtcgggcat cgccaaagag aagatgggca acgccaccag catctacaac
    12721 ctgatgcgca atatcggcgg cagcttcggc atcgccacca tgaccacgtt cctggcgcgc
    12781 cgcggccagg tgcaccagaa ccagctcatc gttcatgtca ccccgtacga tcacggaaca
    12841 caaatgtcgc tgcgcggtat gcaggcctgg ttccaaatgc acggttcgaa ctccgtggac
    12901 gccatgaaga aatccatggg cgcgatttac ggcatggtgc agcaacaggc agccctgttg
    12961 tcgttcgtcg aggcgttctg gattatgggc gtgattttct tctgtatgct gccattgctc
    13021 ttgttcctgc gtaatgcccg cgatcttcat ccccatgagc gcaagtcgaa gggtgggcgc
    13081 gatggtcagc ccaccgacgc gaagccagaa cccgagcccg aactgattgg cgcattccac
    13141 tagcgacgaa gcgcgctcta caatcacccc agtgccagcc atcccggcaa cttcggttac
    13201 gccttaccgc ggacgtgtcg ccccatcccc caccggactt ctgcacctcg gacacgcctg
    13261 cacgttttgg acggcttatc agcgggcgct cgatcaccag ggcacgttaa tcttccgcaa
    13321 cgaagacctc gacccgcaac gctctcagcc taagtacgcc gaggcaatgc tggaagacct
    13381 gcgctggctc ggcattcgtt ggcaagaagg ccccgacgtc ggcggtccgt tcgcgccgta
    13441 tcagcagagc ctgcgtcgcg atttctacct cgccgcctgg cgcaagctgc gcgacacggg
    13501 agccatctat ccttgcagtt gctcgcgaaa agacctcgcg ctgtcggcgt ccgctcctga
    13561 cgatggcgac gacgaaccca tgtacccggg ccgttgtcgc gagaagatcg acgaaactgc
    13621 gaattatgaa gctcccgccg gcgtgaactg gcgccttcgc gtgcctgacg gcgaagttat
    13681 tcagtttgtc gatcagtgtc agggcccgca atcgtacgtg gtgggcgagg atttcggaga
    13741 cttcgtcatc tggcggcgcg atgatgttcc ggcctaccaa cttgccgtgg ttgtcgacga
    13801 tgccgccatg cagatcacag aggtggttcg cggcgcagac ttgctgaagt ccactgcccg
    13861 gcaattgttg ctgatccgcg ctctcacgct gccgacaccc acgtactttc actgcgatct
    13921 agtctgcgac gaggtcggaa tgcggctggc gaaacgtcac gatgccctca gtctgcgggc
    13981 cctgcgcgag ttggggcaca cccctgagca ggtgctggcc atgtgcgatc gtcactagtc
    14041 cgcaatctct cattgcactg aatccccaaa ccggatatcg tttcgccgac accggaggag
    14101 agccatgaaa gtcaactgga gcgcaatcgt attggcggct atcgttcatt tcgggtttgg
    14161 agcggtatgg ttcacggcat tctccaaggc atggcaggcg ggcacccgga tccctccgga
    14221 ggagatgcag gcgtacatgg cccatcccaa cttctggccc tacatcattt cctttctctg
    14281 cagcctggga atggcgctgg ttatctcctg ggtcatgggc ggattctgca agtacaacct
    14341 gtttcgcggc attgtcactg gattgatggt gggcatagtg gcagcactcg ccatgatcac
    14401 cgagatggcg ttcgagatgc gctccagcac gttcatgttc atttccgcag cctatccgct
    14461 gatcggttca gtgctgatgg gcatcatcat cggcgtatgg aagccgaaag gcgtgctggc
    14521 agccagctga ttcgcagacg aagggcggga ggttgattca tgcacggaca cctgcgagtg
    14581 aaagatggtc tagatcgcct gcccggccct aacggcgagc gctttgtcga attgtttaac
    14641 catgagaccc tcagcgtcga actctatgca ccgcgccgca ccgatccgca gcagccgcat
    14701 cctcgcgacg aagtctatgt catcgcaagc ggaaccggcc gctttcttcg cggagaagag
    14761 gaggtcatct tcgaacgcgg cgacgtgttg tttgccgctg ccggcgtatt ccatcggttc
    14821 gtggacttct cccaggactt cgcggcgtgg gttttctttt acggtccgga gggcggagaa
    14881 aaggactcgc actctgagaa atgacctctt acagtacgcc cgtataatct gaggatatgg
    14941 tcaacacgcc ggaagctccc gtcgccaagc agcccaaata cgaaccggtc attggactgg
    15001 aagtccacgt ccagctcctg accaagacca agattttttg tggctgctcg acccagttcg
    15061 gcaatccgcc caacaccaac gtctgcccag tatgtctcgg cctgcccggc gcgctgccgg
    15121 tgctcaaccg gcaggcagtg gagttcgctg tgcgggcgtc gatggcgctc aactgccgga
    15181 tgaacgagcg atccatattc gcgcgcaaga actactttta tcccgatttg cccaagggct
    15241 atcagatttc gcagtatgac aagccgctgg cggagcacgg ctatattgag atcccgactc
    15301 cctctggcgc caagcgtatt ggcatcactc gcgtccacat ggaagaagac gccggcaaaa
    15361 gcctgcacga tggcctggcc gacagcagtc agtggacctc gatcgacctc aatcgctctg
    15421 gggtgccgtt gattgagatc gtcagtgagc ctgacatctc ttcgcccgat gaagcgtggg
    15481 agtacctgac gcgcctgaaa gaaatcatcc tgtacaccgc cgtgagcgac tgcaacatgg
    15541 aagaaggctc gctgcgctgc gatgccaacg tcagcattcg cccggtcggg cagaagaagt
    15601 tcggcaccaa ggcggaggtc aagaacgtga actcgttccg cttcatccgc caggctctgg
    15661 agtacgaaat cgtgcgccag gagcaggtgc tggaatcagg tggcagcatc acccaggaga
    15721 cgcggctcta caacgccggc gagggcaaga cttatagcat gcgctccaaa gagcaggccc
    15781 acgactatcg ctatttccct gagccggatc tgctccccct ggtggtcgat gaagcatgcc
    15841 gtaagcaaat agcggcacag cttcctgagc ttcctgagga caagcgcaag cgcctggtcg
    15901 agcattacgg tatcaccgaa gccgatgccg ccacgttgac ccgcacccag tccatggccg
    15961 accagtttga ggaggctgcg tccgctgcca agaatcccaa gcgcgtcgcc aacctggtgc
    16021 aggccgagtt gctcggtcgc ctaaaggtca agggcctgga gatcgagcag tctcctattt
    16081 cgatgaaagg gattgctttc tcggcggact tggtcgaatc gggcgccatc agtggcaagc
    16141 agctcaaaga gctctacgat cttgccttcg agcgcaacca agatttcccc gccatctacg
    16201 aaaaggaaaa gccgcagcag atcaccgaca cgggtgccat cgagaagatg attgacgagg
    16261 tcatcgcggc caatcctaag cagcttgagc agtatcgcgc cggaaagaag accgtcgcgg
    16321 gcttcttcgt tgggcagatc atgaaagcct caaagggaca ggcgaacccc gcgctggtca
    16381 acgaactgct cagcaagaaa ctcgacggct agcgcgcgcc tgtgtcttgc gaaccacgat
    16441 ttgttaggct ggtggttcgc atgactcaac gtctatccgt tatggccgcg tgcattctcg
    16501 tcggagcagc gctgactggc tgtggaactc ccggcgcgcc gcagttgccc tcgctacaac
    16561 tggcgcggcc ggtggaggac cttaccgcat cgcgcaaggg cacgaaaatc caactcgact
    16621 ggacattgcc tcgcaagaac atcgaccgca ccctggtcaa gaacattccc cagagcctga
    16681 tatgccgcca tgacggcacc gccctgatga gcggatgcac cgtggtagct accgcaccga
    16741 atccgaaacc tgaggagaag cacaagcatg agcaaccgcc cgccctgcac atgaaatatg
    16801 tggatacgct tcctgctgcg ttgggcgagc agaatcccgc aggctttgtc cgttatgcag
    16861 tcgaaatcat gaattcgcgc gaacgtagcg cgggactctc caaccaggtg ctgattccgg
    16921 ttgctccgac cattgcgccg cccgaacagc ttgcagtcaa ggtcgaggcg gatggcgtgt
    16981 tgatctcatg ggtgggagca accgtgccga cggcaccgac gggcctgacg tatcgctatc
    17041 gtgtgatgcg aagtcccgcg ggagcgaacg cctatatcgc gcttgccgat atcgagcctg
    17101 cgtccgaggg cttctacctc gacaaaacat ttggatggga aaccaaatac gattaccgca
    17161 tcacgtcggt gacgctggtg cattcccaaa ccatcaacat gtcagtcgaa ggcgacgact
    17221 cgaaagccac tgaaatattc acccgcgaca tttatccacc ggcggagccg acgggactgc
    17281 aagccgtctt ttcgagcgtt ggtcaaatgc ccttcgtcga ccttacctgg gcccccaaca
    17341 tggagagcga tctggcggga tacaatgtct tccgccgcct cgcaggcggt gctgccgtca
    17401 aactgaataa actactcgtg gcggttccgt cgttccgcga cgacaacgtc gtgcccggaa
    17461 agaagtacgt gtacgctgtt tcggccgtgg atgcgcgcgg taacgaaagt ccgcgctcgg
    17521 cggagaccac ggaagtcgta cccgacaagc aataacggag ttgttatgcg attctgccga
    17581 tttgagtctg acgctgactc tgaaaaggtc gcgcgctacg gcctcatcga tagcggcggc
    17641 actcaggatg taatccgccg cactcttgac caccttccga ccggcgagtc cgatttcgag
    17701 cgcggcaacc cggtggatat tccgctcgct tcggttcgcc tgcttgcgcc ctcatcgcct
    17761 tccaagatcg tttgcatcgg ccgcaactat atcgagcacg ccaaagagtt gaaccatcca
    17821 attcccacgg agccgctaat cttcctcaag ccgccgtcgg ctgtccttgc gcccggcgac
    17881 accatcctgc gtcccgcgga gctttcgcaa cgagtcgatc acgaaggcga actcggcgtc
    17941 gtcatcggca agcgctgtca tgccctagac gaaggcgacg acgtgcgacc gttcattctc
    18001 ggctacacct gcgtcaatga cgtcactgcc cgggacttgc aaaacaaaga tggccagtgg
    18061 tcgcgggcca agggattcga cacattttgc ccattcggcc cgatcgtggt ctccggactc
    18121 gatccatgga gcggggtacg cgttcagacc cgggtcaacg gcgagattcg ccaggatgga
    18181 actaccgccg acttcatttt ccctctggat gtaatcctgc gcttcatctc gcaggtgatg
    18241 actctcgaac ctggtgacct gatcgccacc ggcacacctg caggcgtcag cccgctgcag
    18301 gccggagatg tggtcgaagt tacggtcgag ggtgtcgggt cactccgcaa tccggtgggc
    18361 aacgcctgat ggggccatcg gccatctcaa atggcctgcg aaatctccgt gtattagcga
    18421 acgcaaatcc gcgctatcct aatgcctgac gagtatgagt tccctcggct actatcccat
    18481 cctcgcgtta ggcgccaccg cggccatcgt gggctatgcg ctgctgtcgc gcaaggcgaa
    18541 gccatcgagt gatctcatgg agtacgaaca ccgcaccgag cttacccgcg gcggacgaat
    18601 catcgatggt acggtcatcg acgttcacga acgggaagaa actgggactc agcggcaaat
    18661 gattttgttg gtttacacct atgatgtggc tggcgtctct tacgaagctg cgcaggacgt
    18721 cactcatctg cggcagttca tcgacctgta ttcctgccgg ctcgggctcc ccgcgtccgt
    18781 gaaatacgat ccccacaatc ctggcgattc catcgtgatc tcggagacat ggtcggggct
    18841 ccgcaaatcg ccgattcgcg cgcggtcccg gccagttgcc agctaatccg ccactttagt
    18901 gcaccttggt gccctctcgt ggcatctaat tgagtagcgc gcatctatga atcttcgact
    18961 gacgctgtgt tacacctttg tggttgatac aataaggttt tatgcatggt cttcctcttc
    19021 ccctggcagc gtttgtagcg ggaatcctgt cattcctgtc gccctgcgtg ctccctctgg
    19081 tgccgggcta cgtctcgctc atttcgggcg ctaccgtcga agaccttcag gcgggtgatc
    19141 gccgcatgct gggcacggtg atgttgcact cgctcacgtt cattctcgga tttagcgtgg
    19201 tgtttatcgc gctgggcgca gtcgccaccg gcattggtca gttcgccaac cagtaccaca
    19261 acctgctggc caaggccgcg gggatcgttg tcatcgtctt cgggttgcat ctgaccggct
    19321 tgctgaagat caaggcgctc tatgccgaca agcggttgca cgatgtcaag ggcggttcca
    19381 gcgcactggg ctcgtttgcc gtcggcttcg cgtttgcatt cggctggact ccctgcatcg
    19441 ggccaattct ggcaaccata ctggtttttg ccggggccca ggaaaccgtc ttcaagggag
    19501 tgttgctgct ggcaatctat tcggctggac tggccgtacc gttcttattg acgtcgctgg
    19561 gtgtggaccg gtttctcggt ttctataccc gttttcgccg tcacctgctc accgtcgaag
    19621 taatcagcgg agttttgctg atcgttgtcg gggtgctaat tttcatgaat aatctgaaac
    19681 tgctttcggg atatctggcc ttcctgaatc ggttcgcgct ctaaaggtcg tactcctgtg
    19741 aaacgaaacg ccttggtgtt tgccgcgatg ttcattgcga ttctggcaat gctggcgacg
    19801 ggcaagtatc ttgatcgcac ccaccgccat ggtgccccgg tgttggtggg ggatgtccgc
    19861 ggaatcactg ctcccaactt cgagttggcc acgctggacg gacagagggt caagctttct
    19921 gactatcgcg gcaaagccgt gctgctgaac ttctgggcca cttggtgcgg gccatgtaaa
    19981 gtcgagatgc cctggttcgt cgacttgcaa aagaaatacg gcaatgacgg attggtcatc
    20041 ctgggcatag ccatggacga ttcggaaacc ccgaagattg cgcaggtcgc cagcgaaatg
    20101 ggtgtcaact atcccgtgtt gctgggcacc gacaaggttt ctgaagcgta cgggaatgtc
    20161 gagttgttgc ccaccagttt ttatatcgat cgggagggca aaatcgtcgg caaaggcgaa
    20221 ggtctgctgg gcaggaacga aatcgagcaa aatgtccaga aagcgctggc ttctccagta
    20281 gccggcatga acaaagccca ggcgtcacct acccaagcca agggcgtgaa ggtgccggcg
    20341 atagcacaat gagccggaac ctggtttacc tgctgcttgc tttcgtcgtt acaggggttg
    20401 cgtcttcgca ggagccgggc aggaaagtcc cttcagtgac tctggctccg gtgggaagag
    20461 ttgcgatcaa agcgggcagc accgcgaatg tggaactcga ttttcgcatc ggcagcgaat
    20521 tccatatcaa ttccaacaag cctcattctg agctgctgat tcctaccgcc atgaaactca
    20581 acgccgccga accggtgaac gtggctagcc tgaagtatcc agccgggcag gacatgtcgt
    20641 ttccgtttgc tccggacgaa aagctgagcg tgtacagcgg cgatttcacc atcacggccg
    20701 tgatcaaggc cccgccgaag gcagccaatg ggacctatcc ggtaacgggc gaactccgct
    20761 tccaggcgtg cgatcgcagc gcctgctatc ctccgcgctt gattcctgta aagttcgaag
    20821 ttacggttgt gggccgataa gtcacgacac tcggatattc acaactgcca ttcccccacg
    20881 acttacggcc acatgatttc ggtcagcagc accaccgcat gcgcgattgc cgcgttgtcg
    20941 gtgcccatgc cttcgggggt cttggccttg attcccacct gctcagggtc gatgcccaac
    21001 agctgcgcga cgctctgttg aatcgccttg gcgtgtggac cgatcttcgg cgcggcgagg
    21061 atcaaggtgc agtccacatt ccccaccctg tagcccttct tggcgatttg cttcattgcc
    21121 tcgcgcagaa acacggctga gtcggcgccc ttccattgcg gatcactggg cggaaaatag
    21181 cttccgatgt cgcctgcggc cacggcacca agcaatgcgt cggttaaggc gtgcagtagc
    21241 acgtcgccat cggagtgtcc ggccaatccg ctgtcatgcg gcagcaggac agctccgatc
    21301 ctgagcggga tcccggcttt aaactcgtgc gagtcccagc cgaagccgat ccgcggcaca
    21361 cgttcgactt gcgtgttgaa tgacaaggcg cgtcctatct tgtgggaatg gtcttgcggc
    21421 gaacttcctg cgacaaaaag aactccgcca attccaagtc gccgggagtg gtgatcttca
    21481 agttgcgcgg cgatcccatg acgacatgca ccgtgacgcc agcgcgctca atcagcgagg
    21541 cctcgtcggt ccccagaaat ccatcggcgc tggcatcgtc aaacgctttt ttcagcagcc
    21601 caaagcgaaa gccctgcggc gtctgcgcca tgaccatgcg ctcgcgcgga accgtggcca
    21661 gcaccaccgc gccatccgct gtacggtcca cctgctttac ggtgtcaaca gccggcactc
    21721 cagcaatggc ggagtcgtat ttcttcaccg cctcaatcac gccacggatg gtttcgtcgt
    21781 ccacaaacgg acgcaccgca tcgtgcacca gtacgatgtc gtcaggcgca gcgttgagag
    21841 cggccagtgc gttggcgaca gagtcttgcc ggtgctcgcc gccttccacc acgcgtatgg
    21901 gcttcttgaa gccctcttgt tcgacttgct gcagaaaacc tgcggtctcg ttcttacgca
    21961 gcgcaaccac gatctcgtca acctggctgt ggttggcgaa cttccgcagg gtgtggatca
    22021 aaataggtgt tccctgcagt tcgaagaact gcttgctctt gtggccggcg gatgcggcag
    22081 acatgcgagt gcctaagccg gctgctggga tgatgacaat cactttcata ttggggagcg
    22141 cagtataaac gcccggcgtg acttaattca aatcgcaagg cgaaaaacag ggagccctgc
    22201 ctcccggcaa gactcccgga tggaattcta ctccgccttc gggatgtcag gaacaactgc
    22261 gggtccaggt acaggcaccg gtttccgcaa ctcaggccgt ggctccatgg gacgcggcgg
    22321 catcatgggc gcgcgctcgt cgaacttccc gaaaatcatc ttgcctgccg tcgtctgcaa
    22381 caccgaagtc accgagatgt cgatgttctt gccaatcatt ttgcgcgcgt tgtccaccac
    22441 caccatggtt ccgtcgtcga ggtaggcgac accctggttg tactccttgc cttccttcag
    22501 gatgaaaacc ttcatgactt cgccgggcag cacaatcggt ttcagcgagt tggccagctc
    22561 gttgatgttc aacaccgcca ctccctgcaa ctgtgccacc ttgttcaggt tgaagtcgtt
    22621 ggtgacaatc ttgccctcgt acaccttcgc cagttcaatc agcttgaggt cgacttccct
    22681 cactgcggga aagtcatcct caacaatctg caccgtcagg ctggcaattt tctggatgcg
    22741 ttgcaggatg tcgagtccgc gccgcccgcg gttgcgcttc atggagtcgg ccgagtcggc
    22801 gaccagttgc agttcgcgca gcacaaattg aggcagcaca atcacgccgt cgaggaaccc
    22861 ggtttcggcg atgtcggcaa tccgcccgtc aatgatcacg ctggtgtcga ggatcttgta
    22921 gctcttcttc ccctgcttct cgccgccgaa aataccgccc agggcagcca ggttcagcaa
    22981 atcgccctta cctgcgccca ccaccagacc tatgtaggcc atcaatagca tgaggaagag
    23041 ctgcagaaaa ctctgggtgg gtccgggttg caggctgttg cgaatcacca ggctgaagag
    23101 gtaggcgccg acgatgccga gtatgctgcc gatcaccgcc ccaatgagcc gtttcaggct
    23161 cacgttacgc aggcgaatct cgaacagcac caccgccaga ccgaataaca agcccacgcc
    23221 cgctgcgggc cagcgctcca atcccagggg tctgagttcg taactggaaa gtgctgtaat
    23281 gaaaacgaat aaaacacgta taactaccag gtccaaaaaa cacctccccg gggccggtcc
    23341 tggcgtctac atcgggttcg agaaacgccg aaaataatga gaacaaatat gccgacggct
    23401 cactcccgct cgtaagacgc gatgggcctc gtctaaccaa taacccgggc aagattaaaa
    23461 tatgaaaacg cctctcgtcc gccacacacg gctgataaga gcactgtcct gcgccgtggg
    23521 tgaagtatat atcagattgc cagagcccca atgtggcatc cgacgcccga cagtccgcct
    23581 attccaaaat agaacaggac tccccaccgt cttccagcga gtcgatttgc gaatgacaac
    23641 cccggaattg catggctttc caactaggcg aacagggccc cgaatcctgc ccccgccagg
    23701 gtcacgacca cccaccagcc aaacacgacg cccatgcatg tccctcgctt gacccgggtc
    23761 acgcacgagt agccgatgcc ggtaagaatc agcgtccaga tggtgaacgc gtcaatcgca
    23821 accagcacgg cgtaaagaaa gtgggacgtc gtcgggtcaa ccaatcctcc caggttgctg
    23881 gccacctggt tctgaaatgt aaaactttcg gctgcaccga acccaaccac ggcagtcgcg
    23941 atgagcgtct tcagaatcga tggcaggaag gcgtacatac taatcgctat cgcctgtttg
    24001 aatttcactt cggcgccaaa gccaaagtta aacgttgcca tcagcacgcc cgcgaacacc
    24061 gccgcggcaa tgatcgtcag caggggcgac ccgtaggcga aatatctggt gatcgtgccc
    24121 cctatccgca gttgtgtggc cttctggtcg ggaggcgcct gctcatattt gtccatctgc
    24181 ttcggactca gccgaagctg gttttcatac gccgtgtcca tgccgagttt tttgtcgacc
    24241 acgaacacca tcgctaatcc gaatatcgac atcaccagcc agggcaccca ccaacttgca
    24301 ttgcgccgga tgtcggtaaa cgtcttgctc ggagccacaa acgtgtcgac cacgcgctcc
    24361 atctcggtga gaggtttata ttcggctgga acagcagcaa ttgtgtcggt catgaaaccc
    24421 tccattcggc attgtcgaga ttctgatgtt tgaaggggca cggcttcagc cgcgccgtac
    24481 aaagctcctt attttttgtc attccgagtg ggcgtcagcc cacgaggaat ctgctctccc
    24541 gcgttattcc agtagcctgt gaagtcgggc aggattgtag cattttgcag ccgccgaacc
    24601 acccctatta gtctgctacc cggctttaca cacttgtcac ccgggttgta gggatgcccg
    24661 cccccctttt agactgaact catgcacgtc cacgccagcc ctgcgaccaa tgccgcaact
    24721 tccaaggtcc tgcggatttc gctggtggtt acgctggtgt acatcgtcct gctggtggtg
    24781 gccggagtgc gcgcccacag cctggccctg ctgtccgagg ccggacacaa cctgtcggac
    24841 ttctttgccc tgctgctgtc atggggagcg gtctatcttc agggcaagag tcccgacgcc
    24901 cgcaaaacct ttggctacaa ccgtgctggt gttctggcgg cgttcatcaa cggaacctcg
    24961 ctggtcctga tctcgttcta catctggtac gaagccttcc gccgcctgct gaacccggtc
    25021 gacgtccatg cgcaactcat gatctgggtc gccgccgcag gcgtgatcat gaatggcgga
    25081 atcgccctgc tgctcatgcg cggccgtcgc gacatcaatc tgcgcagcgc catcgtgcac
    25141 gaaatcggcg acgcgctctc aaccgccgcg gtcatcgccg gtggcgtcgc catcgccttc
    25201 accggccagc gctggatcga tcccgcgctc tccatcggca tcggcgtgat gatcctgtgg
    25261 tcgtccatcg gaatcatccg cgagagcctg aatatcctgc tcgaaggcac ccccagcggc
    25321 atggaactgg agcgcattga aagcgcgatc cgcaccatcc ccggcgtcaa tgacgttcac
    25381 gacctgcacg tgtggagcat cggcagcgat actcattcgc tgtcctgcca cgttagcatc
    25441 gccgacatgc gcgcctcgga gagcgaaacc ctgcttcgcc aaattcgcga ccaactcgga
    25501 tcgcgcttcc atattcatca caccaccatc cagttcgagt acgccgtctg cgaagtggcg
    25561 cacggctgcg tcattccctc cagtcccgga catgaacaca cgcatgacca ctaactccgc
    25621 ccgcgggaga agttggttga ttcgcttgtc ccacgctgtg ggaggtgtcg cgctcgttgt
    25681 cgtgattctc gcagcgatgg ggtgcagtaa gaaaggcccg tccgcaccgg cgactgaaca
    25741 accttcgtcg gctgcaacca cagatgcgtc gcccagcggc tggaaactcg acttgaaagt
    25801 cacgccggac cgtcctcgca tggggcgtcc agccacattt gccgtgcaca tcaccgacag
    25861 cctgggtaag cctgtggaga acgcccagct caccggctcg ctcaacatga cactcatgga
    25921 catgggcaag actgccgtca agtttgggcc caagggcaag ggcgattacg aggcgatagt
    25981 cccgagcttc gacatgtcgg gcccatggga actcatcgtc gatgcagcgc agggcacact
    26041 ccgcgcccgc aaggtattcc cgatcacggt attcgattag cggcttcgcc tccttcaaaa
    26101 catcgagtga tccataacga ctacggcacc tccaccagga gcggcacgcg ccgctttttc
    26161 cgtcctgtga ctcgtatcac agggcggggt gaggtggaat gctacattaa atgtttatgt
    26221 ccgctcctga atctctcttt gtacgtgcct gcaaatgcct tcctgtggaa actactccgg
    26281 tctggttcat gcgccaggct ggccgttaca tgaaggaata ccgcgacgtc cgcaagcagt
    26341 attcgctggt ggaaatctgc aagaagcccg aagtcgccgc cgaggtgacg atcactgccg
    26401 ctgaatatct cggggtcgac gccgccatca ttttcgctga cctgctgctg ccgctggaag
    26461 tcatgggcat gccctttcat ttctcaccgg gcgaaggacc ggtgattgag aagccgattc
    26521 gtagcgccgc ggacgtgaag gtactacgta ccgatcgcgc cgccgatttg ggctacgtcg
    26581 ccgaagccgt aggcaaagtg gtaaagcatt tcggctcgaa gctacctgtc atcggattct
    26641 gcggggcccc gttcaccctg gccagctaca tggtggaggg cggcggatcg cgccattacg
    26701 tagaagtgaa gaagctgatg tacaactcgc cggaagtctg ggaggagttg gtcaacaagc
    26761 tggtcgttgt gctggcggaa tacgcagccg aacaggttcg acacggcgca gatgtcatcc
    26821 agattttcga tagctgggta gggtgcttga gcgtggaaga ttatcgtcgc tatgcgctcg
    26881 aaccgacaac gcgattggtg aatcaattgc aaaaagcgac tggcgtgccg atcatctatt
    26941 tcggaaccga cagcaccacg ctgctgccgt cgatgtcgga aaccggcgca gaggtcgtcg
    27001 gactcgattg gcgggtgccg ctcgacgaag gctgggcaac ggtgggacac aagcacgcgg
    27061 tgcagggaaa tctcgaccca gtcacgctgt ttgcgtcgtg gagcgaagtg aagcagcgcg
    27121 ccgaacacat tctgcggctg gcggataaac gtcccggcca tattttcaat cttggtcacg
    27181 gcatcttgcc gctgactccg gtggaaaacg tgaagaacct cgccgacttt gtgcacgagt
    27241 tcagttcgtc ggccaaggcc acgaggaagt aatgagcaaa atggcagttc tgctgatcgc
    27301 gcacggcagc cctgagcggg tcgaggacat tcccgagttc ctgcaaaaca tttcgcgcgg
    27361 gcgtcctatg gcggactcgg tggtgcgcga ggtgcagcat cgctattcgc tgattggcag
    27421 ctcgccgctg acccggatca cccgccagca ggcgcagaat gtggctggcg agctgggcat
    27481 accggtgtac gttgggatgc ggaactggca tccgttcatt gccgatactc tgaagcaaat
    27541 ggcatctgac aatgtgggac aagctgtggc aatctgcctg gcgccccaca actcgcgcac
    27601 cagcgtggga gcctataagg agtctctgtt cggggagaac gctgcgtttc cgattgactt
    27661 cgtggaggag tggcacgacc atcccctgct gatcgaagcg tttgccgaga agctgcgagc
    27721 cggttgggct aaagcctgcg aggagcacgg ctcgcgtttg cctgtactat tcaccgcgca
    27781 tagcgtgccc atgcgcacga taacggaagg cgatccttac gaagcccaga cacgcgagac
    27841 ggctgcgctg gtggcgaaac aagtcccgga gatcggcgag tggaaattcg ctttccagag
    27901 ccagggcatg tcgggcggtg agtggctggg gccgacggtt gaggatacaa tcacgtcact
    27961 gcaaagcgaa ggctgcaccg ggattttcct ccagcccatc ggctttgtct gcgatcacgt
    28021 ggaaatcctg ttcgacattg atatcctgtt ccgccagttc gccgccgagc gcggcatgaa
    28081 actgtatcgc gcggagtcgc tgaatgattc gccgacgttc gcgaaggccg tcgccgacct
    28141 cgtccgcact cgactgaatg ccgggccgcc ttgcgccgga tcgctcgcgg ctgagaagat
    28201 gcaatagcaa tctatgaaac gagtggcaat tattggcggc ggaatttcgg gattgagcgc
    28261 cgctttctat ctggagaagg cccgcgccgc cggggccgac atcacgtaca cattatttga
    28321 aagcggacac cgactcggcg ggtcgatgta ctccgatcgc gtggacggct gcctggtcga
    28381 ggctggtcct gattccttcc tcaccgagaa gccctgggcg ctggcgctgt gcaaggagct
    28441 gggcatcgac gatcaactga tcggctccaa cgatgcgcag cgcaagacct acatcgtggt
    28501 gaagggcaag ctggtcgtca tgcccgatgg cctgatgttc atggttccta cgaaactggt
    28561 tcctacggcg ctgtctcctt tgttttcgtg gggaacgaag cttcgcatgg cgcgggaatt
    28621 gatgcatccg ccgcgtccca tggaaactga cgagacggtg ggcgaactgg tggagcggca
    28681 ttttggtgcg gaagtggtcg accgtctggc cgatccactg ttgtcgggcg tgtacggtgg
    28741 cgatgccaac aagctgagcg cgcgcgccgt gcttccgcgt tttgtggaga tggaagagaa
    28801 gtatggcagc ctgagccgcg ccatgcttgc ggcgcacaac aaaatgcgcg ccatggccgg
    28861 caagcagccg ccacgaccgc tgttcagttc attgaaggac ggcatgcagc agttggtcga
    28921 tgccatcgtg gcgcgccttg atcccgcggc gattcgcatg cgcactcggg ttcgcggtgt
    28981 ttatcctgag aacgatggtt ggcgcgtcgc catcgatatg ggtggaagcg agaacttcga
    29041 cgcgatcatt gtggcaacgc ccgctaacgt tgccggcgcg ttgctcgagg ctgtcgatcg
    29101 cgagctggct gacgacctgc tcggtattac ttacagctca tcggtcacgg tcacgcttgg
    29161 ctaccatatg gaccagctca agcacttgcc accgggcttt ggatttctgg tgccgcgcag
    29221 cgaaggcaca cggatgctgg cctgcacctt cgtgcataac aagtttcccc accgcgcccc
    29281 agcggataaa gggatcttgc gctgcttcct gggaggcgcg cgcgacgagg ccgtactgaa
    29341 cctcaccgac gacgacatcg tggatacggt acgggccgag ttgaagaaca tcgtgaagtt
    29401 agacgcgcgt ccgatgttcg accgtgttta ccgctggcgg ggcgccatgg cgcaatacga
    29461 gtctggtcac atcgcgcgcg tcgagcgcat cgaaaagcga gtcaaagaga ttcccggcct
    29521 cgcgctggcg ggcaacgcgt ttcacgggat tggcgtgccg gactgcatcc gcgaagggat
    29581 gaacgcggcg aacgccattg tgcaagtagc gggcgtcacc gcgattccgc aggcgcgata
    29641 agggctcgcc cggggaccgc aatccctaca tcacgatttc tgtagcccgc cgcgtgatcg
    29701 ggtgctgcat ttcgtcgaga tcgcccttca ccaggtttaa tccatagacg caggactcga
    29761 actcgtgcgc cagttttccg aacagtggcg ccagccgcgc atactcgaac tgatcgaact
    29821 tggaaacgat gaagtagctc tccttgccgg ccttgatgaa gtcgacaaaa ttctccaggc
    29881 gctgccgccg caggcgaaag tgattgatgc tctcgggaaa catgcccgac aggaacagcg
    29941 tgtagtcgcc gatgtgcttg cggacctggc gctcgcggtc gaacgacggc gctggaccat
    30001 agatgggatc ggcctcgagc agcatctcgc ccacgtcatc gagcggacgc tccttcgcat
    30061 tgtgcagctt gaacatctgc tcgacttcgc aaaattccgt caatacgttt accacgtagt
    30121 cctgcacctc ggaatcacgc agcccgatgt cgcggtcgaa gtggtggtgg atgagttcgg
    30181 tgaacagcgc ttgcagcgag tgcgattcag gaatcatgct atttgtttcc cccactgaca
    30241 atccggttac aaccggttgc gttgctgttg tgatgcgttg atagctgact tcgtttcatt
    30301 gtgcaagtgc taaccgcaac caccgtgact ttgttggtgc gggctgtggg cgtgtcgctg
    30361 ccgagaatcg ggttgaacca atacgaaaat catgcgcaat tgcgcctcat ttacaggcgt
    30421 tgccggagtg ctttggttgt cctgctagca gagcggctgg tttgttgcca aaatgcatcg
    30481 gcgaagcgcg agtgcctgaa attcccgctt gtggcacaat tttggattcg tgaggaggca
    30541 gtgatgcaac gacaaaacat atcgggcaac tcgccctatg aaccgaaggt cggattttcg
    30601 cgcgcagtga aagtcggccc gtttgtctcg gtcgcaggaa ccgtggcaac tggagctgat
    30661 ggaaagattg tcggcggaaa aaacatgtac gcccaggcag tgcagacgct gaagaacatt
    30721 gaacgcgcgc tggcgcaggc gggggcaagc ttcagcgacg tggtgcgcac ccgaacatat
    30781 gtcaccgacg tcagccgctg ggaagaagcg ggccgggcgc acggtgaagt gtttggcgat
    30841 attcgtaccg cggcgaccat gatccaggtc gtcgcgatgg tctcgcccga aatgctaatc
    30901 gagatcgagg ccgacgccat catcgccgca gagctgacct agttgcgaag cggaaaagca
    30961 aaaggccggc gcgatgccgg cctccgcaac ttcaaaaata caatgcctac ttcttgggcg
    31021 gtgcgatggc gtctttcaca gccttggcca cgcggaactt caccacggtc ttcgcggcaa
    31081 tcttgatggc ttcgccggtc tgcggattgc ggcccatgcg tgccttgcgg gccgatttca
    31141 ccaggcgacc caacccagga attacgaata cgccattctt cttggtctcc ctgatcgcga
    31201 cgtcggctag gtattcgatc aactccgcta cctgcttgtt gctcagtgaa tgcttctcgg
    31261 cgatgtgccg aaccagctgt gtcttggtca tgcctgctgc cattaggtat tcctccttga
    31321 atcattgaac agtgaaatgc tcaatttgac cgtatcttat aacatccgcg cacggctgca
    31381 aaacgtttta accatatttt atgcgggttt tccacagccc ccacctctcg gaccttgtat
    31441 ccatgcacct ctgggagggt tcctagttag gcaacaatcc cattttggta cgagtggagc
    31501 accgcacatt tcggccctcg gagaaggggc catcgtggca ccgacagggg tctccttcgg
    31561 catcctcagg gcagcttctt ccacttcgtt cccaagcgtc accacgctcg gaatgacaaa
    31621 ccgcgtacct gttactatcg gctctttact cgaatgcaca tcagccacgc cacaattctg
    31681 ttcctcgccg gcctggcagc aggcgcgatc aactccgtag ccggcggtgg aagcttcgtt
    31741 acattcccgg cattgctttt cgtgggcatt ccgccggtaa acgcgaatgc ctcgaatgcc
    31801 gtcgcggtct ggcccgggca agccgtcagt atcggggcgt atcgcagcca gttccgcaat
    31861 ctggcgtggt caaccatgac gccgctgatc atcaccgccg ttctgggcgg cattctcggt
    31921 ggctgggttt tgctgaaaac gccccagacc atcttcatgc acctggtgcc atggctgatc
    31981 ctggccgcaa ctcttatctt cgccatgagt ggacgcgtga cgcgttgggt gcgccagcgg
    32041 accgccagcg gggaacacca tgagttctcc aacgtccggg cagtgtcgat ccagattttt
    32101 attgcgttct acatcggata cttcggggcg ggcgccggga ttctgatcct tgccatgctg
    32161 gcgctgctgg gcatggacga aatccacacc atgaacgcgc tgaaggcgct gctgacgacc
    32221 gtttccaacg gtgttgccat ggtgttgttc gtcgttgccg gtgccgtgta ctggccggag
    32281 acgatcgtga tgggcgtggg gtcgatcctg ggagggtatt ttggagctta cttcgcgcaa
    32341 aagaccaagc ccgagtacgt gcgtgcgatt gtgatcggaa tcggcttcac gctgtcgatt
    32401 tactatttcg cccggcagat cttgagctag aggctagcgc gcgagtcctc gcctgcttca
    32461 ctcttagccg gaactgtggt catgtggcga tagcggattt tcatgatcag caacgggaac
    32521 gccaggaaaa atgtgatggc gttgggcagg ataagaggaa gcgagcgaat cagaaaaccg
    32581 taagccaccc acagcacgag gccaacattc aggctgagca gcatgatgag cgagatgtcg
    32641 cggaccgact tcgtgcgaaa cgaatgcagc gcctgcggta agtaggccag cgtgcacagg
    32701 gtgcccgcgc cgaagcccac gataggaacg aaattcatat ttcagcttag cattgatcgc
    32761 cacgtcgaaa ggtcgacttc gggttacgct cgcccacgat tcagccacca aattccgaag
    32821 ttgagataac tggcataggt gacccacagc agatacggga cgagcagcag cgcggcgact
    32881 tgcgtaagcc agtggaacac cacaatgttg aaaaggatgg ccagccagag aatcaatatt
    32941 tccgcgaaag ccacgccggg cagacgcgcg ccgaagaaga ttcctgacca cgccatgttg
    33001 agcgccaact gcccgaaaaa cagcaccagc gccgaaccac caccgcgcca gccgtaaccc
    33061 cgccacacca accacgcaga ggtggccatc aggatgtaga ggaccgtcca cactggcccg
    33121 aagacaaatc ctggtggcgt gccgggcggc ttgcggaggc tgggatacca ctctcgcacg
    33181 ccacgggcgg tgaggaacga cgccagcccg gcagcgccaa ggcagatgaa cagaaacagg
    33241 atgagcatca accacggctt cgcgtccaca gcagcatttc ctttctcgtt aagtgtgtct
    33301 tctcgaaaac gcgcctaccc tggcgtcggc tctagccgat agtaagcacg gtcttgccgt
    33361 agttggcgcg ctgcaatagc aggcggtgcg cttccccggc ctcctccatt ggcaaagtat
    33421 ggccgatctc gggatgcagc ttgccttgct cgatccaggg ctgcatcgcc ttcaggcaat
    33481 cgttcatcaa ggcgtggtca ttcgccagga cgctcagcca caagccgtgc gcgctgaggc
    33541 tgcgtgccat catcgctccc acgtcgaact tctgacgctc accggtggca gtaccgtaaa
    33601 tgatgacccg tccgaattgc cggcaacaac ggagactctt ggcggtgtgt tcgccgccga
    33661 gcatttcgaa gaccgcatcc acaccttcgc cgttggtgag ttcgctgatg cgtgtctcgt
    33721 aatcgacttc gcgatagttg ataccgtgat cgagtcccag cttctgcacg cggtccagtt
    33781 tctcagtggt cgaagcggtg ccgtaggtct cgacgcgaag cagcttgcca atctgaaccg
    33841 cggctgtccc gactccaccg ccaacggcgt gaatcagcac gcgacggcgg cgttcgcctt
    33901 cgacgtacac gggctcgatg gcgtcagaag ttatgccggc tttccaatag gcgagatacg
    33961 cggtgaagaa attcaccggg aacgctgccg actgctgcga ggtccagccc ttgggttggg
    34021 gccagatcaa cttccgggaa acggcaactt tctccgccgc cgcgccgtat tgcgcgtaac
    34081 ccatcacgcg ctcttgcgtt ccctcgacga tgccggcgaa ttcacgtcct gaaatgaagg
    34141 gtggtggcgg cgtgctcttg taggcgccgc gagtggaaag agtatcggcg aaattcaccc
    34201 cgacggcttc gactcgcacg atgacttcgc cagagtttgc gattgggtcg gggagttcgc
    34261 gcacttcaag aacttcagga ccgccgagac gggtgacgac aacagctttc atggctccac
    34321 tcccgcagcg agtgtaaaac attggagaaa tgtaagtcgg tgacgcgctg cacaaatgct
    34381 aggaggtcgc gggaacctgc atgaagtcca gtgcgcggaa aatgtagttc ttcagctcgc
    34441 ggcgatgcac cactgcatcg agcattccgt gctgcaagag gaactcgctg cgctggaagc
    34501 cttcgggcag tttctgccgg ttggtctgct cgatcacgcg cggtccggcg aaaccaatca
    34561 gcgcacccgg ctcggcaatg ttgaggtccc cgagcatggc gaaagacgcg gtcgttccac
    34621 cagtcgtggg atcggtaagg acggaaatgt agggaacctt ggcgtcgtcc atgcgggcca
    34681 gcgcggcgga tattttcgcc agttgcatca ggctgaccac gccttccatc atgcgcgctc
    34741 ccccggatgc agagacgatg atgagcggaa tcttctcggc cgtggcccgc tcaatcgcgc
    34801 gggtgagcgt ctcgccaaca accgcgccca tcgatccgcc gatgaacgcg tactccatgg
    34861 cgctgacgat gaccggcttg ccgttcaact tgccgcgagc attgatgatg gcatcgttca
    34921 gcccggtatc gctcttggcg cgcttgaggc gatcggcata ggacttcagg tcggtgaact
    34981 tcagagggtc gttcgagatc aggttgccgt cttcggtttc atacttgccg tcgtcgagta
    35041 gataggcaag gcgggtgcgg gcgtcgatgc ggaagtggcg gtcgcatttt gggcagacat
    35101 ggaagttctg gtcgaggtcc tttttccaga tgatctgacg gcaatcatcg cacttgaccc
    35161 aaaggccttc cgtcttgacg cgcttctcgc ccgaagtgtc gagctcgccc tgttcccgct
    35221 taaaccatgc catagtcgtt cctcagtgta gccgacggga gcgagggttg tcgccgctca
    35281 gtactcgatc ccgcgctgcg cttcgatgcc cttggtgtag gcgtgcttca cttccttcat
    35341 ctcggtaacc gtgtcggcga tttcgacga
//
LOCUS       DASRKK010000264         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1289641, whole genome shotgun sequence.
ACCESSION   DASRKK010000264 DASRKK010000000
VERSION     DASRKK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289641"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFC15_05825"
     CDS             <1..441
                     /locus_tag="VFC15_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM09713.1"
                     /translation="WSAPAFISIGGGNFGFQAGGQSVDLVILFMNDKGVQGLLSSKFE
                     LSGEASATAGPVGRHASAGTDWKMNTEALSYSRTKGLFAGVAVDGAKIQQDNDSTVAF
                     YGKNVSFRKTLSGDVHAPAGSNPFLAAVRAAVNKANQHEAAEKK"
     gene            866..1963
                     /locus_tag="VFC15_05830"
     CDS             866..1963
                     /locus_tag="VFC15_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM09714.1"
                     /translation="MSIFDQRTAKVLATICAFVAVGAFLYEVRHTLVVILFAVLFAYL
                     LEPVVYRIERSRLARSSRWLAILETYLAIGAILAVFGILFGPKLVEDTRLLSMSLPGL
                     LEKVTTGKIVWQFGNRYGWSYDTQLKIEQFIASHQAEILARSGQAGAAVAKALQSVIW
                     VVLIPILAIFFLRDGRQFSETLLHILGQRNQRRFLRLIMTDLDVMLAHFIRVQLILAG
                     ISLVAYSVVLTALRFPYALALGFAAGAMEFIPVVGPLVAALAIVFVGFLTGYPHLWAL
                     ILFFACWRVVQDYVVSPRVMGGTLELHPLAAIAAVLMGGELGGVLGVYLSIPVVATIR
                     IVWVRWQKYSAVIAMAAAQGDIPESARDAVT"
     gene            2082..2912
                     /locus_tag="VFC15_05835"
     CDS             2082..2912
                     /locus_tag="VFC15_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM09715.1"
                     /translation="MSSVLLIGKETDTVRSIAQALAAVDCHCERASGGAATIRRLRQQ
                     SFDVVITDPESTIDEGLALIEEMRDIRPGMKAILLTDSSTPEEVIAALRARVFVCFSA
                     PFDVDEIASFACRAATDSDWRTEIEVLSAQPDWVSLRANCRILTAERVVTFLGELCSE
                     LPEPPHDNVMLAFREILLNAMEHGGAFNRHKVVEVSAVRTERTIVFYVRDPGMGFRRE
                     ALAHAVAGGAADPAAHLERRAEMGMRPGGFGILLAEGLVDELIYSEAGNEVLMIKHTA
                     "
     gene            complement(3034..3570)
                     /locus_tag="VFC15_05840"
     CDS             complement(3034..3570)
                     /locus_tag="VFC15_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HZM09716.1"
                     /translation="MDRKWKVLLAVGTGVILTKLVLADSKPLLPTVSSVDLTRYVGKW
                     YEIARYPNWFEKEDVGDVTAEYTPRPDGKITVVNSCRRADGTTKTSKGEAKVENKATN
                     AKLKVAFFKPFYGKYWIIDLAADYSYAVVGEPSRKYLWILSRAPQMSGKTYEKITRRV
                     QELGYDPAKLIKTPQAQI"
     gene            complement(3857..>4181)
                     /locus_tag="VFC15_05845"
     CDS             complement(3857..>4181)
                     /locus_tag="VFC15_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZM09717.1"
                     /translation="TLTGDKVVNFQKEDLGKIEHLMIDLESGRIAYAVLSFGGFLGMG
                     DKLFAIPWTSLKVDTVEHRFILNVDKELLKAAPGFDKEHWPDMADRAWGAKVYTYYGA
                     KPYWN"
BASE COUNT          812 a         1161 c         1241 g          967 t
ORIGIN      
        1 tggagcgcgc cggcattcat ttcgattggt ggaggcaatt ttggcttcca ggctggcggt
       61 cagtcagtcg atctcgtcat cttgttcatg aatgacaagg gtgtgcaggg gctgctctcg
      121 agtaagttcg aactcagcgg cgaggcgtca gccaccgcgg gtccggttgg aaggcatgcc
      181 tcggcaggca ccgactggaa gatgaacacg gaggccttga gctactcgcg caccaaaggc
      241 ctatttgctg gagtcgcagt cgatggcgcc aagatccagc aggacaatga ctccacggtt
      301 gcgttttacg gcaagaatgt ttctttccgc aaaacgctgt caggcgatgt acacgccccg
      361 gcgggcagca acccgtttct cgcggcagtg agagctgccg tcaacaaggc gaatcagcac
      421 gaagctgccg aaaagaagta gttagagtta gcccgtacgc ctgccgaatt gtgcacgccg
      481 gcaatcgcac gtcgcggcgt cgctcgggga tggcctcgat cagttttgga ccgggaggga
      541 tgcggtatta caggatagct tcccatttcg gctgctcttt cggcgaatga acggtgcaag
      601 caacgcgtag gaatgtgcca ccctgcaccg atgcagtctg gattggtatc cgaaatcgag
      661 aggatggagc ggtatctaag actctgtctc cgagccaagt cagttccgag aatctcagcg
      721 ttggctagag acgcggcatg ggccttttca ggagccgcga ggaccaagaa gacttcgaga
      781 cgaagcgaag tgagggacgg ttcgacctac tcggattctg ataacggaga tcccggctga
      841 gccgaaatta caatcaaact attcaatgtc tatcttcgat cagcgtacgg ccaaggtgct
      901 ggccacaatc tgcgcctttg tcgccgtcgg ggcctttctc tacgaggtgc gccacaccct
      961 cgtcgtgatc ctcttcgcgg tgctcttcgc ttacttatta gaaccggttg tctaccgaat
     1021 cgaacggtcg aggcttgcga ggagctcgag atggctcgca atcctggaaa cttatttggc
     1081 gattggcgcg attctcgcgg tgttcggaat tctgttcgga ccaaagctgg tggaggacac
     1141 tcggctgcta agcatgtcgc tgcccggatt gctggagaaa gttacaaccg gaaagatcgt
     1201 ttggcaattt ggcaatcggt acggctggag ctacgacact cagctcaaga tcgagcagtt
     1261 tattgcgtcc catcaggcag agattctcgc tcggagtggc caggcaggtg cagcggttgc
     1321 gaaggcgttg cagagtgtga tttgggttgt tctgatcccc atcctcgcta tcttcttctt
     1381 gcgcgacgga aggcagttct ccgaaacctt gctgcatatc cttggccaac gcaaccagcg
     1441 tcgctttctg cgattgatca tgaccgatct cgacgtgatg cttgctcatt tcattcgcgt
     1501 gcagctcata ctggccggca tatcgctagt ggcatacagc gtggtgttaa ccgcgcttcg
     1561 gtttccgtat gcgttggcgc tgggattcgc ggccggcgct atggagttta ttcccgtcgt
     1621 tggtccgctt gtggccgcgc tggcgatcgt cttcgtcgga tttctgacgg gttatcctca
     1681 cttatgggcg ctgatcctgt tttttgcctg ttggcgagtg gtgcaggatt atgtcgtatc
     1741 tccgcgggtg atgggcggaa cactagaatt gcatcctcta gcagcaatcg cggctgtgct
     1801 gatgggtggc gaacttggcg gagtattggg cgtttacctt tcgatcccgg tcgttgcaac
     1861 catccgcatc gtttgggtgc gttggcagaa atattctgcg gtcattgcga tggcagctgc
     1921 gcaaggagac atcccagagt cggcccggga cgctgttact tagagcagca ttgcgaggtt
     1981 ccgacaggag agagaacccg ttcaaagttg cataagtgcg tgacagattc ggtcgcaggc
     2041 gttctccgag agttgtgatc gattgtactc aggagcaaag gatgagtagc gttcttctta
     2101 tcggcaaaga gacggacacc gtccgttcga tcgcgcaagc gctcgccgcc gtggattgcc
     2161 actgtgaacg cgctagtggt ggcgcggcca cgattcgccg tttgcgtcag cagtccttcg
     2221 atgtagtcat caccgaccct gaaagcacga ttgatgaggg cctggcgctg atcgaggaga
     2281 tgcgcgacat tcggcccggc atgaaggcca tccttctcac cgattccagc acgccagagg
     2341 aagtcatcgc tgcgctgcgc gctcgcgtct ttgtctgttt cagcgccccg ttcgacgttg
     2401 acgagattgc cagcttcgcc tgccgcgccg cgaccgactc cgattggcgc accgaaatcg
     2461 aagttctctc cgcgcagcct gactgggttt cgcttcgtgc caactgccgc atactgaccg
     2521 ctgaacgcgt ggtgaccttc ctcggagaat tgtgctcaga attgcccgaa cctccacacg
     2581 acaacgtaat gctggcgttc cgcgagatcc tgctcaacgc tatggagcac ggaggggcct
     2641 tcaatcgcca caaggtggtg gaggtatccg cggtgcgcac cgaacgcacc atcgtgtttt
     2701 acgtgcgcga tccgggtatg ggctttcgcc gcgaggcgct ggcgcacgct gtcgcaggcg
     2761 gggctgcgga ccctgccgca catctcgagc ggcgagcgga aatgggcatg cgccccggcg
     2821 gatttggcat tctgctggcg gagggcttgg tggacgagct gatctacagt gaagccggca
     2881 atgaagtcct catgattaaa catactgcgt gagaaacggg ttcgccgatc ggcggcaaca
     2941 ctctcccttg actccccggt actttgttgc aaagcgctca ttttgcaggc gcatcggtgg
     3001 cgatgcgcgc actctggagc ttaaggggcc tgctcagatc tgcgcttgtg gtgtcttgat
     3061 cagtttcgcg gggtcgtagc cgagttcctg tactcgtcgg gtgatcttct catacgtttt
     3121 cccgctcatt tgtggtgcac ggctcaaaat ccaaagatat ttccggcttg gttcacccac
     3181 gacggcatag ctgtagtcgg ccgccaggtc gatgatccag tacttgccgt aaaacggctt
     3241 gaagaaagcg accttcagct tggcgttggt cgccttattc tcaaccttgg cctcaccctt
     3301 ggacgtcttg gtcgttccgt cggctctgcg gcaggagttc acaaccgtga tcttgccgtc
     3361 cggccgtggg gtgtactcgg cagtgacgtc gccgacatct tccttctcaa accagtttgg
     3421 atatcgcgct atctcgtacc acttgccgac gtaacgggtg aggtccacgc ttgaaacggt
     3481 gggtagcagg ggcttcgaat ccgccagcac cagtttcgtt aggatcacgc ctgtaccgac
     3541 agcgagaagt accttccact tcctgtccac ttgctatacc tctttccaat acgaggctgg
     3601 attccgcgtt ccggctgacg caggaccgtg gaactaccgc gaccacaggg aatacatttg
     3661 ggcagagaat gcgcccatcc aaccagccgt ggctggaacc caagcacccg ctaaacgtcc
     3721 gcagataatg gcccaaggcg atgtcatgat tcagcactca ccttgcagtc ggcaacgccc
     3781 gccgttgcgt cagcgcaacg gccgataacg acggctagtc ttgtcgcgtc tagccgtcgc
     3841 ggttcagcct gcgcgcttag ttccagtagg gcttggcgcc gtaataggtg tagaccttgg
     3901 cgccccaggc gcggtcagcc atgtcgggcc agtgctcctt gtcgaaaccg ggagcggcct
     3961 tgagcaattc cttgtcgacg ttcaaaatga accgatgttc gaccgtgtcg accttcaggg
     4021 aagtccatgg aatggcaaag agcttatctc ccatgccgag gaacccgccg aacgaaagga
     4081 ccgcgtaagc gatgcggcct gactccaggt cgatcatcaa atgctcgatc tttccgaggt
     4141 cttctttttg aaaattcacg actttatcgc cggttagcgt a
//
LOCUS       DASRKK010000265         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_801211, whole genome shotgun sequence.
ACCESSION   DASRKK010000265 DASRKK010000000
VERSION     DASRKK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801211"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1060)
                     /locus_tag="VFC15_05850"
     CDS             complement(143..1060)
                     /locus_tag="VFC15_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZM09718.1"
                     /translation="MESTNQSRLFEVLFDHAEPSLIADDAYAPYGNFGFPPPPTDRPW
                     IYANFVQSLDGITTLLGTHASGGEISQSPEDRWLMDLLRAHADGLLMGMNTLLEEQRL
                     RGPQSRGIVFQVADPELRALRDRLGKGRERNIFVTRAIDLDLSRHKVFDGDVVDAAIL
                     TSPAGAERLRAQGSHPHVAILAAGEGERFDLPRAIGKLREELGVKYLLCEGGPTLYGS
                     LARADLVDEKFMTVSPIEVGQLVPAEQERSASEQATSVLLRPTVFGGPGYTRENLTHW
                     TWLSCRKAGDHQFSRYRRKGRGQGLGVSG"
     gene            complement(1379..2806)
                     /locus_tag="VFC15_05855"
     CDS             complement(1379..2806)
                     /locus_tag="VFC15_05855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM09719.1"
                     /translation="MSYIQFLGAAGVVTGSKHLIHVDSNGSRGLQILVDCGLFQGQKE
                     WRERNWQDLPVPANSIDAVILTHAHLDHSGWIPRLVKQGFHGPIYATPATIDLCSILL
                     PDSGHLQEEDAAFYNKTRTSKHVPALPLYTMEDAQQCMTLFRPVAFGEAKQLTGALSF
                     RFVRAAHILGSAMTEVTLASNGKSSKLLFTGDIGRVRDDQIAPGKVVHSGPTEGEDCD
                     LLVMESTYGNRLHPHTDPRPELAALVRTTLQRGGSVIVPSFAVERTQKFLFLLKELME
                     TGQVPRVPVHADSPMAIKAVQIFLKHTEEFSPETKALIEKYGSPLEWPNFYFDATQDE
                     SKKINESKYPAIIVSSSGMVTGGRVLHHLMQRLPDPKSLVLFIGFQAPGTRGFTIKSG
                     APEVKIFGQQVPIRAQVAALEQFSDHADTPELLEWLHTFRRPPEETYLVHGEPPAAAQ
                     LRDAMVRELGWDVQVAAYREKVPVG"
     gene            complement(2920..3696)
                     /locus_tag="VFC15_05860"
     CDS             complement(2920..3696)
                     /locus_tag="VFC15_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09720.1"
                     /translation="MPEPFASSRSKLAWAKENLLPDLDRRIREFHNLDPYAKVVEPDP
                     QRPGWEIHKIKLVQPFPEAFGNITSDIVVNLRSVLDNAGYAIADAVGKPDARNCAFPF
                     ASDVDNMARSIGRSADLPKEIQSLFCGFQPYRGGDDLLWALNELCNGNKHKFVTPMQA
                     IMWRGRASVKGFGRPFDMPDPHKWDSASNEMVVVRLGPIVAPNATWDYEFDFLPFIAI
                     HEIPVVSGHPVLGVLDELCLKVESVLAAIEAESRRLGFVK"
     gene            complement(3890..>4173)
                     /locus_tag="VFC15_05865"
     CDS             complement(3890..>4173)
                     /locus_tag="VFC15_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09721.1"
                     /translation="VESAYTRPLLSEANKSFLVTATIENRGGAGAEVPVLIQTPSGEK
                     SVRVVVKAGEKGTGRIEVPVAPDKIVVNDRSVPEANLGNNVYEIPGKQP"
BASE COUNT          831 a         1244 c         1186 g          912 t
ORIGIN      
        1 tttgcccttg caaaaacatg tccctcaggg gctgaagccc aaagtcgttg ttgacccgtt
       61 gcggcacggc tgaagccatg ccctgataca aaacgattta tgggatagct cggatagctt
      121 ctaggtattt cccaaacaaa ccctaaccac tgacccctag cccctgacct ctgcctttcc
      181 gcctgtagcg actgaactgg tgatcgccgg ccttgcggca gctcagccag gtccagtgcg
      241 tcaagttctc tcttgtgtat cccgggccgc cgaagacagt cgggcgtagc aaaaccgaag
      301 tggcttgctc gctggcgctg cgctcttgct cggcaggaac cagctgtccc acctcgatgg
      361 gcgagaccgt catgaatttt tcgtccacca gatcggcacg cgcgagactg ccgtacaagg
      421 tcggcccgcc ttcgcatagc agatacttca cgcccaactc ttcgcgcagc ttgccgatgg
      481 ctcgcggcaa gtcaaagcgt tcgccttcgc cggcggcaag gatcgcgaca tgcggatgag
      541 agccttgcgc ccgcaatcgt tcagcgccgg cgggagaagt aagaatcgcg gcatccacca
      601 cgtcgccatc gaagactttg tgccgcgaga gatcgaggtc aatcgcgcgc gtcacgaaga
      661 tattgcgttc gcggccctta cccaggcgat cacgcagcgc ccgcagctcc gggtcggcga
      721 cctggaagac gatgccccgg ctctgcggac cgcgaaggcg ctgttcctca agcagggtgt
      781 tcatccccat cagcagtccg tcggcgtgcg cccgcagcag gtccatcagc catcgatcct
      841 cgggcgactg cgagatctcg cccccggagg catgcgttcc cagcagcgtg gtgatgccgt
      901 cgagcgactg cacgaagttg gcgtagatcc acggccggtc ggtaggcgga ggcgggaacc
      961 cgaagttgcc gtaaggcgcg taagcgtcat cggcaatgag gctcggttcg gcgtggtcga
     1021 atagtacttc gaacagtcgg ctctggttgg tgctttccat cagtgagatt ccagaggcga
     1081 ttctatctga cgtcccgaaa atggtttcct tcgcgtaaac gggttttcac cacagaggca
     1141 caggggagag cgcttggcat ttggttctta gagcaattcc agagtggtcg taccttgggt
     1201 agtgcgggcc ttcagccggg gcccgcgtta agcccgcccg cgcaattcct agcttcagcc
     1261 gctgaggtcg tcgacctcag cggctgaagc gatttttctt cctgctcctg gacgcgggcc
     1321 tgaaggcccg cactacccgt gcaagcggat acgccaatcc tagaagcgtg acagccgctt
     1381 atccaaccgg cactttctcg cgataggctg ccacctgcac atcccatccc aactctctca
     1441 ccattgcgtc gcgcaactgt gccgctgcgg gcggttctcc gtggaccaga tacgtctcct
     1501 ccggtggacg cctgaacgtg tgcagccact ccagcaattc cggcgtatcg gcgtgatcgc
     1561 tgaactgttc cagcgccgcc acctgcgcgc gaatcgggac ttgctggccg aaaatcttca
     1621 cctcgggagc gccgctcttg atggtgaatc cccgcgtgcc cggcgcctgg aagccgatga
     1681 acagcaccag gcttttcggg tcaggaaggc gctgcatcag gtggtgcagc acgcgcccgc
     1741 cggtgaccat gccgctcgac gacacgatga tagccggata cttcgactca ttgatcttct
     1801 tggactcgtc ctgcgtggca tcgaagtaaa agtttggcca ctccagcggt gagccgtatt
     1861 tctcgatcag cgccttcgtc tcgggactga actcttccgt gtgcttcagg aagatctgca
     1921 ccgccttgat cgccatggga ctgtcggcgt gcaccggcac tcgcggcact tgtcctgtct
     1981 ccatcaactc cttgagcagg aacagaaatt tttgtgtccg ctcgacggca aacgacggga
     2041 caatcacgct gccgccgcgc tggagcgtgg tgcggaccaa tgccgccagt tcaggacggg
     2101 gatcggtgtg cggatgcaag cggttgccgt aggtgctttc catgactagc aggtcgcagt
     2161 cttcgccctc ggttggaccc gagtgcacaa ctttgcccgg tgcgatctgg tcgtcgcgga
     2221 cacggccgat gtcaccggta aagagcaact tcgatgattt gccgttcgag gccagtgtga
     2281 cctcagtcat ggctgagccg aggatgtggg ctgctcgaac aaaccggaac gacagtgcgc
     2341 cagtgagttg cttagcttcg ccgaaggcca ccggacggaa gagcgtcata cactgctgag
     2401 cgtcttccat ggtgtaaagg ggcaacgccg gcacgtgctt gctggtccgc gtcttgttgt
     2461 agaacgccgc gtcttcttcc tgcaggtgtc cggagtcggg aagcaggatg ctgcacagat
     2521 cgatggttgc gggcgtcgca tagatggggc cgtgaaaacc ttgcttgacc aggcgcggaa
     2581 tccatcccga atgatcgaga tgcgcgtgcg tcaggatcac ggcatcaatc gagttagcgg
     2641 gcaccggcag gtcttgccag ttccgctccc gccattcttt ttgtccctgg aagaggccac
     2701 aatccacaag tatctgcagc cctcgcgagc cgttgctatc tacatggatc aaatgcttcg
     2761 agccggtaac cactccggcc gcgccgagaa actgaatgta gctcaagagg tcctcaccct
     2821 tacgaattag agagcgttgt ggtcgcgggg atgcgactca agatgataac agtgtctccg
     2881 aaaagatgat tcaccacacg gtactgcgct actttgaatt catttaacaa atccgagacg
     2941 ccgagactcc gcttcgatag ccgcgaggac actctcgact ttcaagcaaa gctcatccag
     3001 gactccaagg actgggtggc ccgaaacaac aggaatttcg tggatcgcta taaacggaag
     3061 gaagtcgaac tcgtaatccc atgtcgcgtt aggagcaacg atgggtccga gcctgaccac
     3121 taccatctca ttgctagcgc tgtcccattt atggggatcg ggcatatcga acggcctacc
     3181 gaatccttta acggaagcgc gtcctcgcca catgatggct tgcatgggcg taacgaattt
     3241 gtgtttattc ccgttacaca gttcgttcaa tgcccacaag aggtcatcgc ctcctctgta
     3301 cggctggaac ccgcaaaaca gcgattgaat ctctttcggg aggtcagcgg aacgcccgat
     3361 agagcgagcc atgttgtcaa cgtcactggc gaacggaaaa gcgcagtttc tagcgtcagg
     3421 ctttccgact gcgtcggcga tggcgtaacc ggcattgtcc aaaacgcttc gcaggttaac
     3481 gacaatgtcg ctggtaatgt tgccaaatgc ttctggaaag ggttggacaa gttttatctt
     3541 gtgaatctcc cagcccggtc tttgcggatc gggttcgacg acctttgcgt atggatcgag
     3601 gttgtgaaac tcccggattc tccggtcgag atcggggagg aggttttcct tagcccatgc
     3661 gagtttgcta cgtgaactgg cgaatggttc tggcacgatt tcaaccccat tatctacgcg
     3721 gctataggct ccgcgctggc ttcgtagctt tcccacaacc caccacagag gcacagagaa
     3781 cacagagaac aagcacaagg atctgatctg tgctcatccg tgaaaatccg tggttatttg
     3841 tattgttttt tctctgtgtc ctctgtgcct ctgtggtaga gctttttctt cacggctgct
     3901 tgcctggaat ttcataaaca ttattgccca ggttggcttc cggaacgctc ctatcattaa
     3961 ccacaatttt gtcgggcgcc accggcactt cgatgcggcc cgtacccttt tctcccgcct
     4021 tcaccactac gcgaacactc ttctccccgg aaggagtctg aatcagcacc ggcacctccg
     4081 ctcctgcgcc gccgcggttc tcgatggtcg cagtcaccag gaagctcttg ttcgcctcgc
     4141 tgagcagggg gcgggtgtac gccgattcca cgt
//
LOCUS       DASRKK010000266         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_19459, whole genome shotgun sequence.
ACCESSION   DASRKK010000266 DASRKK010000000
VERSION     DASRKK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19459"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFC15_05870"
     CDS             <1..255
                     /locus_tag="VFC15_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09722.1"
                     /translation="FEGHNENRMRAVQRSLAASLLHRFESAHRLHTVLGHALPVDEET
                     RKSYAALQQWLIESSVPLFLFLPWTLEDEVEGAQAFFERA"
     gene            268..540
                     /locus_tag="VFC15_05875"
     CDS             268..540
                     /locus_tag="VFC15_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09723.1"
                     /translation="MYSPEDTICGFVAGLSGRPIADVQPGNILLDDLGLKPHDLDTLA
                     RLIENEYGVKPSADEMLGWKRMQDVTIFIRLNVQPASCAPTGHPTR"
     gene            800..1738
                     /locus_tag="VFC15_05880"
     CDS             800..1738
                     /locus_tag="VFC15_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM09724.1"
                     /translation="MIAILGAGGTVANELVKLLAARNQAFRIVGRNPRPTHGATETLA
                     ADLTDKDQTIRAVAGSSIVHLLVGLKYDHKVWQEMWPRVMSNTIEACKRADAKLIFFD
                     NVYMYGKVSGPMTEATPFNPCSKKGEIRANIATTLINGWKSGDLTGMIARAADFYGPD
                     SRNGVPNLLVFEPFSRKQKASWLVNDSVPHSYTYTPDAARSLLLLAERATAWNQTWHV
                     ATTPNPSTGKEFVTMVAKEFGAEPKYRVLSRPMVRLAGWFNPLVSESYEMLYQSDSPY
                     LFDSSKFANEFGFAGTPYADGVRTAAASFTGKSHGA"
     gene            complement(2029..2157)
                     /locus_tag="VFC15_05885"
     CDS             complement(2029..2157)
                     /locus_tag="VFC15_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09725.1"
                     /translation="MSDGREEREEREKRREQEERENREDRINPDHVAKWEQERVDS"
     gene            complement(2294..2572)
                     /locus_tag="VFC15_05890"
     CDS             complement(2294..2572)
                     /locus_tag="VFC15_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09726.1"
                     /translation="MPKKIGADKQRESRTLGEALGAEITRLRVDRGWSQSKLAHDLGY
                     TERYIGQLERGTKSPTLRTLADLAKAFSTELSAIIRAAERRHRNSAKR"
BASE COUNT          649 a          879 c          804 g          629 t
ORIGIN      
        1 tttgagggcc acaacgaaaa caggatgcga gcggttcaaa gaagcctagc agcgtcatta
       61 ctgcaccgat ttgagtccgc acatcggctg cacacggttc ttgggcatgc gctccctgtt
      121 gacgaagaga cgagaaagtc gtatgctgca ttgcagcaat ggctgattga gagttccgtt
      181 cccctattcc ttttccttcc ttggaccttg gaagacgagg tcgagggcgc gcaggcattt
      241 ttcgagcgcg cttgaaagga tcccgccatg tattcacccg aagacaccat ctgcggattc
      301 gttgccggcc tcagcgggcg accaatcgcc gacgtgcagc ccggaaacat actcttggat
      361 gacctcggtc tgaagccgca cgaccttgac acgctcgccc ggttgatcga gaacgagtac
      421 ggtgtgaagc cttcagctga cgagatgttg ggctggaagc gaatgcagga cgtaaccatc
      481 ttcatccgcc taaacgtcca gccggcttct tgcgcgccaa cgggacatcc aactcggtag
      541 tctcggcaga cgaaacactc gtgagggtta aaggcgtcaa ccacccttat tccatgccgt
      601 acgagcactt cgatatctct attgccgcgg cctcaagcgg cccctaaacg agatctggcc
      661 cgaagcgaag aattgggact gagagcgtag cagtccggca cggacgttac gcgtaagatg
      721 aaccgagcgg taagtggctg atcgagaggt aggtgtggag caatggccat acttgtctcc
      781 gcccaaaggc aggatttgaa tgatcgccat tcttggagca ggcggtaccg ttgcaaacga
      841 actcgtcaaa cttctcgccg caaggaatca ggctttccgc atagttggcc gcaatcctcg
      901 gccgacacat ggcgcgaccg aaacactcgc cgccgatctc acggataaag accagaccat
      961 ccgcgccgtt gccggttcca gcattgtgca tctgctcgtt ggtctgaagt acgatcacaa
     1021 ggtctggcag gaaatgtggc cgcgtgtcat gagcaacacg atcgaagcct gcaagcgtgc
     1081 tgatgccaag ctcatcttct ttgacaacgt gtatatgtac ggcaaggtca gtggtcctat
     1141 gaccgaagcg acgcccttca atccctgcag caagaagggc gaaatacgcg ccaatatagc
     1201 cacaacattg attaatgggt ggaagtctgg cgatctcacg ggcatgattg cgcgcgcagc
     1261 tgatttctat ggtcctgact cgcgcaatgg agttcctaat cttctcgtct tcgagccttt
     1321 ctcgcgaaag cagaaagcct catggctggt gaatgattct gttccgcact cctacaccta
     1381 cacgccggat gctgcgcgaa gcctgctact gctcgccgaa cgggcgaccg catggaacca
     1441 gacctggcat gtggccacca cgcccaatcc gtccaccgga aaagagttcg tcaccatggt
     1501 cgcgaaagaa ttcggcgctg agccaaaata ccgtgtcctg agccggccca tggtcaggct
     1561 tgccggatgg tttaatccgc tggtatcgga atcctacgaa atgctctatc agagtgattc
     1621 gccctacctc tttgactcaa gcaagtttgc caacgagttc ggcttcgcag gcacacccta
     1681 cgccgacggc gttcgtaccg cggccgcctc ctttacgggc aaatcgcacg gtgcctagaa
     1741 caagagacgt cacaacgcta tgaccatccg cgatggaatg cccacattgc cacgctgccg
     1801 ttgcgatcgc ccaagcgaac gcggagttgg tgttcaacgt tgcgatcgag tgacgggcaa
     1861 tcctccagta accggatagg gaagagtggc cgaaattgca gaccaccgga tgcaccttct
     1921 ccccgctccg ggctgatcgt taccgaagct ttagctaagt cgatccggat tgcttccgcc
     1981 agccctcgaa gcctttcccg tctgcagcag ttcagcctcc gtagcacttt aagagtcaac
     2041 ccgctcctgc tcccacttag ccacgtgatc ggggttgatc cggtcctctc gattctctcg
     2101 ctcttcctgc tcgcggcgct tctcgcgttc ttcgcgctct tcgcggccat cgctcatacc
     2161 ccctcctctc ttcagtataa aaaccaggtc cgccgcgcgg tcaatcacga catcgtggtc
     2221 gtagctgagc tcatcgatcc gaatagatcc aaaagagaat ccaccgaatc acataacctg
     2281 ctggcggtga acatcagcgc ttcgcgctgt tccggtgtcg tcgttcggca gctctgatta
     2341 tggcggaaag ctccgtggaa aacgctttgg cgagatcggc gagtgttcgt agggttgggc
     2401 tcttggtacc gcgttccagt tggccgatgt agcgttcggt atatcccaga tcgtgcgcta
     2461 attttgattg gctccagcct cgatcgacac gcagtcgggt gatctcagca ccgagcgcct
     2521 cacctaacgt gcgggactca cgctgtttat cagcaccaat ctttttgggc acgctcccat
     2581 agttgtcgtt tgacgctaaa tgattgagga actatagttc gtggtcgatt aaggacgaga
     2641 gttttcgatc tcaaaatgag tcgccggctc ccgccggttg ggcaggaacc cgaagttcaa
     2701 gcccggcgga aatggcgatg acaagttgct tccagcatcg gcgaaatcga ctaaaatagg
     2761 gcggcaggca gctggctccg gaacgacgat cgacgacacg cagccctgca tccccatccc
     2821 aggtactccg gtgtgaagaa atccaaacgc gaattcgtgc gcgactcgac cgcccgcctt
     2881 cagcgggccg cccgccaacg cgccgacgag gaggcccgcc ggattccgtg gcagcggctc
     2941 tacgaagttc gcaatcaata c
//
LOCUS       DASRKK010000267         7084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_312235, whole genome shotgun sequence.
ACCESSION   DASRKK010000267 DASRKK010000000
VERSION     DASRKK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312235"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFC15_05895"
     CDS             <1..1298
                     /locus_tag="VFC15_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZM09727.1"
                     /translation="PQADGNRIEVIAPTDLSISPGKIVGLLGPSGSGKSTLLRMLTGL
                     SKPSSGQVLWHHKPMDGHIPNVSIVFQSFALFPWLTVLENVEAPLIARGAGAIERHKR
                     ALKMIDTVGLDGFETAYPKELSGGMKQRVGFARALVVEPEVLFMDEPFSSLDVLTAEN
                     LRSELLELWLNKKMPTTAIFIVTHNIEEAVLLADRVIVLGRNPGRIRSDFEIDIPQYR
                     DRKSPHFLAYVDYIYKTLTRPEEEAVIPGKTAGQSADGQPRTRTKFQMLPHARPGGIA
                     GFMELLTDRNGSEDLYKLADQLSMDVDDLLPIVDASVMLGFAVLQEGDVTITPEGRAF
                     GEADIQGQKAIFRKAALDHIALLRLIENTLHAKRDHAIGEEFFRDILDEHFSADEVER
                     QFETALNWGRYAEIFDYDSESKRLFHNEEADTVPSLGNR"
     gene            1298..3052
                     /locus_tag="VFC15_05900"
     CDS             1298..3052
                     /locus_tag="VFC15_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZM09728.1"
                     /translation="MARGTWQPIRILGKGPWSLLPAQTWSVLTDFVIFAAGLSVFYVI
                     LLLGRTWLGPFNPQVEISRDPTKLPLYAAYSLLRIAVAYVLSLAFTLVYGYVAAYNPK
                     AERIMIPLLDILQSIPVLSFLPGVMLAMVALFPHHQIGVEMGAILLIFTGQVWNMAFS
                     FYSSLKSIPRDMREAAKIYRFNWYQRFTQFELPYAAIGLVWNSMMSVAGGWFFLMACE
                     MFVLGARDFRLPGLGSYLQTAASAGDTQAIFWGLGVMILVIVLLDQIVWRPVIAWAEK
                     FKMEQVESADVPHSWFLNVIQGSRGLIRLRKRVIRPLQERLAMHFVRQHDSAEDAAPV
                     PWKVWTGRAIAAIVVLAVLYAVTKAIAMLTGLDGSEYRELLTGAGATFLRVVAALVIG
                     AAWTIPAGVAIGFNPKLARIAQPLAQIAASVPATALFPIVLLVLIRIGGGLGLGSIVL
                     LLLGTQWYILFNVIAGAMAIPTDLKEASDLFKFNRAQRWRKLILPGIFPYLITGMVTA
                     SGGAWNASIVAEYFRLKDKTFTTVGLGATISRASDAGNFDLLLASTIVMAIVVVTVNR
                     LVWRRMYRLAATRYTLET"
     gene            3144..3410
                     /locus_tag="VFC15_05905"
     CDS             3144..3410
                     /locus_tag="VFC15_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09729.1"
                     /translation="MGTRPAIFKWRQTEPGLILCAVRWYLRYSLSLRDVEELLEERGL
                     NVDHTTVWRWVQYYGRMVKKFARFEKARLAASTSLFGLPSPPSR"
     gene            4948..6045
                     /locus_tag="VFC15_05910"
     CDS             4948..6045
                     /locus_tag="VFC15_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09730.1"
                     /translation="MERLVNDLIVTLARVKRENPREFDTQLTNELRALRNALQSEPTQ
                     WEFYVPVIGFAPSELPFTVGKVEFYQADGSGTQEYWRRACELNSAYEGRRPEEHILAD
                     FPAQFGNRTIGRVRVSAVDLSAAHNRAMKAMRRTLDCINFFADRNKGLALHVKGDVEQ
                     GQLLAAAIQLSHPARPTGRITRIAPWRGLPIRQISRRPGFGRMSELLEKKTLTKLEDR
                     ILKALQWAGRARVELRTEESFLLFAVALETLLLKDSGNPVDLLYQLKLRAAHLVTGLD
                     FDSRKLTVKQIGQLYNTRSKIVHTGKFDVTDSDLYLIDEYTRIGLFIVLVREPFCSMT
                     AEEDFEAWFEARLLGQAQTPDEASDATAAPG"
BASE COUNT         1472 a         1935 c         2061 g         1616 t
ORIGIN      
        1 atccgcaggc cgacggcaat cgcatcgagg tgatcgcccc cacggacctc agcatctctc
       61 cgggaaagat cgtgggtttg cttggtccat cgggctcggg caaatcgacg ttgctgcgta
      121 tgctgaccgg cctgtctaaa ccgtcttccg ggcaggtgct atggcatcac aagcctatgg
      181 acgggcacat tccgaatgtc tccatcgtct tccagagctt cgccctcttt ccctggctca
      241 ccgttttgga gaacgtggag gctccgctca tcgcgagagg tgcaggtgca atcgagcggc
      301 acaagcgggc gctgaagatg atcgataccg tcggtctcga cggattcgaa actgcctacc
      361 caaaagaact gtcgggagga atgaagcagc gcgtcggctt cgcgcgcgca ttggtggttg
      421 agccggaggt gctgttcatg gacgagccgt tctcgtcgct cgatgttcta accgccgaaa
      481 atcttcgctc cgagttgctt gagctatggc tcaacaagaa gatgccgacc accgccatct
      541 tcatcgtgac ccacaacatc gaagaagccg ttttgctggc ggaccgtgtg atcgtgctcg
      601 gccgaaatcc cgggcgcatc cgttcggact tcgaaattga tattccgcag tatcgcgacc
      661 gtaagtcacc gcactttttg gcttatgtgg attacatcta caagaccctg acacgtccgg
      721 aagaagaagc tgttataccc ggcaagaccg ccgggcaatc cgcagatggc cagccccgta
      781 cgcgcaccaa attccaaatg ctgccccacg cccgtccggg agggattgcc ggcttcatgg
      841 agttgctcac cgatcgcaac ggttccgaag acctctacaa actggccgat caactgtcga
      901 tggacgtgga cgatcttctt cccatcgtgg acgcctccgt catgttggga tttgcggtcc
      961 tgcaggaagg cgatgtcacg attacgccgg agggccgggc cttcggagag gccgatatcc
     1021 agggacaaaa ggctatcttc cgcaaggcgg cactggacca tatcgcgctt ctgcggctca
     1081 tcgagaatac gttgcacgcc aaacgcgacc atgcgatcgg ggaggaattc ttccgcgata
     1141 tactcgacga acacttcagc gccgatgagg tggagcgcca attcgagacc gcactgaact
     1201 ggggccggta cgcggagatc ttcgactacg actctgaatc caagcggctg ttccacaatg
     1261 aagaggcgga tacggttccc tcgctaggaa atcgctgatg gcgcgcggta cctggcagcc
     1321 tatccggata ttgggcaagg gcccgtggtc gttgctgccg gcgcaaacct ggtcggtact
     1381 aactgacttc gtgatctttg ccgccgggtt gtcggttttc tacgtaattc tgttgttggg
     1441 gcgcacctgg cttggcccct tcaatcctca ggtcgagatt tcacgcgatc ccaccaagct
     1501 accactgtac gcggcttact cgctgctgcg gatcgcggtg gcctacgttc tttcgcttgc
     1561 gttcacgctg gtgtacggtt acgtcgcggc gtataacccg aaggccgagc ggatcatgat
     1621 tccgctgctc gacattctgc agtcaattcc tgtgcttagt tttttgccgg gagtgatgtt
     1681 ggccatggtg gctttgtttc cccatcatca gatcggggtc gagatggggg ccatcctgct
     1741 gattttcacc gggcaggtgt ggaacatggc cttcagcttc tattcgtcgc tgaagagcat
     1801 cccgcgcgac atgcgcgagg ccgccaagat ctatcgcttc aattggtatc agcgcttcac
     1861 ccaattcgaa ctgccgtacg ctgccatcgg cctcgtgtgg aattcgatga tgtcggttgc
     1921 gggcggatgg ttctttctga tggcctgcga gatgttcgtg ctaggcgcgc gcgacttccg
     1981 tctgccgggg ctaggttcct acttgcaaac ggcggccagc gcgggcgaca cccaagccat
     2041 tttctggggc ctcggagtca tgatcttggt gattgtgctg ctcgatcaga tcgtgtggcg
     2101 accggtcatc gcttgggccg agaagttcaa gatggaacag gtcgagagcg ccgacgttcc
     2161 acactcctgg ttcctcaatg tcattcaggg ctcgcgagga ttgatccggc tgcgcaaacg
     2221 tgtgattaga ccgttgcaag agcggctggc gatgcatttc gtccggcagc atgacagcgc
     2281 ggaagatgct gcgccagtgc cgtggaaggt gtggaccggt cgcgccatcg ccgcgattgt
     2341 cgtactcgct gtgttgtatg ccgtgaccaa agccatcgcc atgctgaccg gcttggatgg
     2401 ctcggagtac cgcgaactac taaccggcgc gggagcgaca tttctccgcg tagtggcggc
     2461 gctggttatt ggagcggcgt ggacaattcc cgcgggagtg gcgatcggct tcaatcccaa
     2521 actggcgcgc attgcgcagc cgctggcgca aatcgccgca tcggtgccgg cgaccgcgct
     2581 gtttcctatc gtattgctgg tgctgatcag gattggcggc ggcctggggc tggggtcgat
     2641 cgtgctgctg ctgctgggaa cacagtggta catcctgttc aacgtaatcg ccggcgccat
     2701 ggcgattccc actgatctga aggaagcctc tgacctgttc aagttcaatc gcgctcaacg
     2761 ctggcgcaag ctgattctgc ccggcatctt tccctatctg atcacgggta tggtgacggc
     2821 ttccggcggc gcttggaacg ccagcatcgt ggccgaatac tttcggctta aggacaagac
     2881 gttcacgacc gtcggtttgg gcgcgacgat ttcgcgcgcc tcggatgccg gtaacttcga
     2941 tctgctgctg gcttccacca tcgtgatggc gattgtggtg gtgaccgtga accgcctggt
     3001 ctggcggaga atgtatcggc tcgcggctac gcgatacacg ctggaaacgt gaaaaaccag
     3061 tgaatagtgg gcaatggata gtgaatagag ggatgtgttg caactttcag ggcggctggc
     3121 tggtgacgtg aaagaattgc gaaatgggaa cccgacccgc gatcttcaaa tggcggcaga
     3181 ccgaacccgg cctcatactg tgtgcggtcc ggtggtatct tcgctattct ctttcgctgc
     3241 gcgatgttga ggaactgctg gaggagcgcg gtctcaatgt cgatcacaca acggtctggc
     3301 gctgggtgca gtattacggt cgtatggtca agaaattcgc ccggtttgaa aaggcgagac
     3361 ttgcagcctc gacaagtctc ttcggattgc cttctccacc gtctcgataa ggcctacaat
     3421 ccagctttcc cggtagggtc aagaaattcg aaacggttgg aaaggcgagg cttgcactcc
     3481 gagttgtctc ttgcggttga tcgaggctgg aagggtgaaa accatgcccc taccagccag
     3541 gagtccgaat ttgaacgcct atgcggaacg ctgggtgaga tcggtgaaac aggaatgcct
     3601 gtcgaagctc atcttgttcg gggagaggcc gctgcgccga gccctgcagc aatatgtggc
     3661 acattaccac gaagagcgca attcaccagg aggctgcgag acattacagc ggaagacgtg
     3721 ctcggcttcc ggcagtggcg cacaacgggc ggagtgggtc cggcaatcgt gcttggtccg
     3781 cgcagagttt ctactttgag gatgcgctaa gcactgaact tgctgctgag agtttcgctt
     3841 tgttaacgct gcttgtcgac cccgaggatc gccccgctct gagatactgg cttggagggg
     3901 gtcagcaaga ctgccgtcgc aggccgtatg cccgcttgcg agagcattgt gaacagacag
     3961 gacaatctcc cagggccgct ctagagttgc ttagaatcgg cactctgcaa ctgccataca
     4021 cccaatcgtt ggtcgcgagg tacaaccaat tacaacagcg tgtcgccacg cttatgcctt
     4081 tggacatccc gcagctggtt gatgcattgt ttctcgacgg taatgccgac gtggcttccg
     4141 tgcggcaagt tgctctcctt attgcaccga acgtccaaac tcagcgcgaa ctgttggatc
     4201 aattacggac ggatataacg cagccagagc tcccgggaac acaggggcct gccatccgca
     4261 tcatgagctt acacaagtcc aagggtctca cggcccggct tgttgtaatt gctgggtgtg
     4321 tatctggcat cctaccgagc attgattcca gtgcgtctct ggccgagcag aacagacagc
     4381 gacaagaaca gcggcggctt ttttacgtag ggataacgcg aagcactgaa accctgattc
     4441 tcagcagtgc agtccgcatg ccgtatggcc aggcgatgca catgcgaatg ccagtgattc
     4501 ctggtcccta cgggatggcc atcttacaag cgagcccgtt cttgtcagaa ttgggagcag
     4561 atgctccgaa tccgattgac gcagcgactt ggcgggcgac tgttgggttt tgactcttgc
     4621 ggcaagtgac agtctattta caacatcgat cttcgaacga tcgttgttgg tgttgacgcg
     4681 ccgccagagc agcaaataag ttgtgcgacg ggagccaatc gcatgccaga cttgaacggc
     4741 gcacttaggc gacttgccga gtttgttacg aacttagatt tcgctacaga gccacctcca
     4801 gccttcattt cgtctgagcc attcgtgatg ttgtcgaagg atgacgcgac cctattcatt
     4861 cgtggggaag attccgacaa ttacggtgaa tgtgtcagag ggatctttga cgcagtttca
     4921 aaaagagaaa tcattagtcg ggattccgtg gagcgactcg tcaacgatct gatagtaacg
     4981 ctggcacgcg tgaaacgtga gaatccgcgt gagtttgaca ctcaactcac caatgaactt
     5041 cgagctctta gaaatgctct gcaatccgag cctacgcagt gggaatttta tgtgcccgtc
     5101 atcggatttg ccccttctga actgccattc acagtcggca aggttgagtt ctaccaggcg
     5161 gacggcagcg gcacgcaaga atactggcga agagcatgtg agctaaactc agcctatgaa
     5221 ggaaggcggc ccgaggagca catactcgcg gattttcccg ctcaattcgg caacagaaca
     5281 atcggtcgcg ttcgcgtttc agcagttgac ttgagcgccg ctcacaaccg cgctatgaag
     5341 gccatgcgtc gcaccctcga ttgcatcaac ttctttgccg acaggaataa gggactggcg
     5401 ctgcatgtga agggcgatgt ggagcaggga caacttttag ctgctgctat tcagctctcg
     5461 caccctgcaa ggccgactgg ccgcattacc cggattgccc cgtggagggg gctgccgatt
     5521 cggcagattt cgcggcggcc tggctttggc cgaatgtcgg aactgttaga gaaaaagact
     5581 ctcactaagc tggaagacag gatcctaaag gcgctccaat gggctggacg cgcccgagtg
     5641 gaactgcgta ccgaggagtc attcctgctg tttgccgtcg cccttgagac gctgctactc
     5701 aaggacagtg gtaatccagt tgatctcctc tatcagctca aacttcgtgc tgcccacctt
     5761 gtcactgggc tggacttcga ttcccgaaag ctaactgtaa agcagattgg gcagctctac
     5821 aacacgcggt cgaagatcgt ccacactggc aagtttgacg ttactgattc tgacctctat
     5881 ctcatcgacg agtacacgcg tatcgggctc ttcatcgtgt tggtcagaga gccgttctgc
     5941 tcgatgacgg cagaagaaga cttcgaagct tggttcgagg cacgcctact cgggcaagcc
     6001 caaactccag atgaggctag cgatgccacc gccgcacctg ggtaaaggta atcagcgtgt
     6061 gcgacagccg tggaggtagc gcgatgtccg gggattatta accacgtcgc acgaataacc
     6121 gctttgtttt taccgcgagc agagcatgtt aaaactgcac ggtttctgct agtacgtcgg
     6181 tcacagcttt tgcctgctct tgctttgtca aatctggcga agccggatgg catcatcacg
     6241 aagattaatc acctgggcaa ggtttgcggt gcaggctaaa ctgagcccaa gagccgaatt
     6301 gagacagact aatgtcgtga ttcgtccgtc gtggtgcggg cgagttcgtt tcatgcgcgt
     6361 ctgacaagca gctggcattg aaatcgatgg aaccatataa gcttaggcgg tggagatcga
     6421 cttcgttatc acatgtagga caatttttta cttgtgctag gccaggaaga tccaaaggtg
     6481 ctcgcgatca cgtgccagat gatcttgtac atcaatgggt ccgagggctc ccaggcgaca
     6541 cccataccgt gattctctct cttctcgggc gcaaacatgg gcaagacggt gggagtgagt
     6601 tctcgttcta ctccttctgt gggcctttgg atctctccga ggaacgccgt cggcgcccct
     6661 catttcagga atggcttgat cgatggcaca ggaagcggtg tattcaggtc ctggaacacc
     6721 caacatatga cttccgcccc gttccgacgg agacactgga taccgtcgca tctgttatcc
     6781 gctccctatt gtcggagggc cgaaccgtcg ttgtaatgga ctcaggcggg gaaacccgca
     6841 cgaggcaagt ctgccagcac atggggttcg tcgaagacac tcgaatggct tgagtcgcgg
     6901 agcagttcgg caccctgcca cacgtcggcg gtctatcagt tctcgttcta cccgtatggt
     6961 caaacatctc aaacactttg aaaaggcttg gtttccagtc tcataatttc ctcgcccagc
     7021 tgctttctga aatctcaaga gcatgtacaa tccgggttcg acgatcaact ttagggaggc
     7081 tctg
//
LOCUS       DASRKK010000268         6876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1192138, whole genome shotgun sequence.
ACCESSION   DASRKK010000268 DASRKK010000000
VERSION     DASRKK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192138"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFC15_05915"
     CDS             <1..253
                     /locus_tag="VFC15_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09731.1"
                     /translation="GPLDPQLITKQLRAALDNAEAFVTRMPSEKLGLLFLQGSKIVQP
                     DPTRLESYQTHAGQRRGQWPTNSEITAAMLERYTKKPGR"
     gene            397..519
                     /locus_tag="VFC15_05920"
     CDS             397..519
                     /locus_tag="VFC15_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09732.1"
                     /translation="MICADFLAGASIDGGNPEILLQSISRFYKFLPGPQQQAFL"
     gene            538..792
                     /locus_tag="VFC15_05925"
     CDS             538..792
                     /locus_tag="VFC15_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZM09733.1"
                     /translation="MKKVSGKSPRLRLDPTSYRDLHRQVLERDGWRCQACGSMQNLQV
                     HHLKFRSHSGSDEEQNLITLCAECHEQMHREADYRKARLR"
     gene            1151..1363
                     /locus_tag="VFC15_05930"
     CDS             1151..1363
                     /locus_tag="VFC15_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09734.1"
                     /translation="MVVGESSTHGARSLATIHDAVSDLEQQANARLIASVPSMHRAAV
                     KLLCAWGTEDAPEAMEELAVILSQAV"
     gene            complement(1901..2860)
                     /locus_tag="VFC15_05935"
     CDS             complement(1901..2860)
                     /locus_tag="VFC15_05935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM09735.1"
                     /translation="MSTYLITGIAGFIGSSIARELVRRGERVRGIDNFSTGKPENLVL
                     ISSAIDFREVDLLDMRGVSEACQGVDYVIHQAALPSVPRSIADPLSCNRSNVDGTLNL
                     LVAARDARVRRIVSASSSSVYGDTPHLLKSEDLTPNPISPYAVSKLAGEYYMRSFHAV
                     YGVETVSLRYFNVFGPYQDPKSMYTGVMAIFIPKMLRQERPTIYGDGEQSRDFTYIDN
                     VVKANLLACHAPADKVAGRYFNIAGGRRVTINETYVLLQQLLGYANAAFYAPERSGDI
                     KHSLADIRLAKQHLGYRPSVEFEEGLNKTVEWYRSQALLPVTA"
     gene            complement(3359..5260)
                     /locus_tag="VFC15_05940"
     CDS             complement(3359..5260)
                     /locus_tag="VFC15_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZM09736.1"
                     /translation="MLLRLRFWFILAFDTILFAASLIGAWLLRFEFTLPQRDILFAAL
                     PILVLMRLAALGRFKLLRGYWRYTGISDIADIVKATALGSLGFLVIERWLLGIKAFPI
                     SIYCLEALLVVGGLCGARLFSRMLAQSVRGAWRGRSEKTAIIVGAGDAAERLLNDLPR
                     CGYMAVGLVDDEPAKIGGRIHGVSVIGSIDDLPRLVRKRAVEEVLIAIPSATGRQMRR
                     ITALCEESGARFRMIPGMSDLINGKVTAEQLRDVKLDDLLGREPIHFDLQAVRQQLTG
                     KVVMVTGAAGSIGSELCAQLLYYKPSKLVCLDQDESALFFLEQKIGDAAAKVCAEYCV
                     ADVADRQRMKGLISAAGVDVIFHAAAYKHVPMMETNRSEAVRNNVLALMSLLDVAEQC
                     GCADFLMISSDKAVNPTNVMGCTKRVCELIMAAKPNSKMRRVSVRFGNVLGSQGSVIP
                     VFQEQIRKQGIVTVTHPDITRFFMTIPEAVSLVLQAFTIGKHGEILVLEMGKPIKIVD
                     VARTLVRLCGKSEDDVEIVFTGLRKGEKLHEELFYASESAFRTDVEKIFCTSSKLMSW
                     PVLHEHLRELEGMVYASTDTMIREKLKQIVPEYRYVEEAVPIEVLPSHIAGREAKLTL
                     ISAIAVAND"
     gene            complement(5343..6596)
                     /locus_tag="VFC15_05945"
     CDS             complement(5343..6596)
                     /locus_tag="VFC15_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZM09737.1"
                     /translation="MATNSKSVQHQPALKGKASVYKSLFSPWPYFAPDEIEAAVAVLQ
                     SGNVNYWTGEEGRLFEKEFAELNGCKHAILVSNGSVALEGALRALGVGPGDEVITSSR
                     TFIASASCAVAVGAQPVMADVDRHSQNITADSIRKVLSSRTKAIVPVHLAGWPCEMDA
                     ILELAAEHGLKVVEDCAQAHGATYKGRPVGSMGDVAAFSFCQDKIMTTAGEGGMVTTN
                     SHDLWEKMWAYKDHGKSYDAVYRKEHVPGYRWLHESFGTNWRMTEVQSAIGRVVLRKV
                     SHWLSIRRKYARTLSNCFAQLPGLRVTYPPVHVDHSYYKYYVFVRPEQLRPGWSRDRI
                     VEAINAEGVPCFTGSCSEIYLEKAFPADWRPKDPLPVAHELGETSLMFLVHPTLTEEA
                     IHATCATVKNVMRVAAHRKTQPRAS"
     gene            complement(6655..>6876)
                     /locus_tag="VFC15_05950"
     CDS             complement(6655..>6876)
                     /locus_tag="VFC15_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09738.1"
                     /translation="LGGGVTVHSFAHVGIGAIALPGSEIGEGAIIGAGAVILDSVASG
                     TTVVGIPARLLHRAETRKTQFRVTGELSE"
BASE COUNT         1563 a         1977 c         1824 g         1512 t
ORIGIN      
        1 gggaccgctc gatccccaac tgattacaaa gcagctacgt gccgctctcg acaatgcgga
       61 ggccttcgtg acgcggatgc cctcggagaa actgggcttg ctgtttttgc agggcagcaa
      121 gatcgtgcag ccggacccca cacgcttgga aagctaccag acccacgccg gacagaggcg
      181 ggggcaatgg ccgaccaaca gcgaaatcac cgccgccatg ctcgagcgtt acaccaaaaa
      241 gccaggccgc tagaacatat ggcatacggc catgcttgct catagaccga tgaccagacc
      301 gctgccctgc aacttactgc ctggaagcgg gccgcatgca aaaccgtttt ccaggcgatg
      361 ctggggtcgg acaagtctcg agggtactgc ctggaaatga tctgtgcgga cttcctcgcc
      421 ggggcctcga tcgacggcgg caacccggag atcctgctgc agtccatctc ccgcttctac
      481 aagttcctgc ccggtccaca acagcaggct tttctctgag gtcaaccaga acgcttcatg
      541 aagaaggtca gcggaaaaag tccgcgactt agactcgacc cgacgagtta tcgagacctg
      601 catcggcagg tgctggaacg cgacggatgg cgctgccagg cttgcggcag catgcagaat
      661 ttgcaggtgc atcatctcaa attccgcagc cattctggca gcgacgagga acagaacctg
      721 atcacgctat gtgccgaatg ccacgaacag atgcatcgag aagccgacta ccgcaaggcc
      781 cggctgcggt gactcgcctc gttgcatggt aaaaaaatgc ttacttcgct gatcacgaga
      841 atccccaaca gggcttgcgc agcttgcgga ttacatgaaa cgcgacgggg gcccgggcat
      901 gctacagcaa tacgatagcg attacctgct cgcgcggtgc ctggtcaccc gcaacggctg
      961 caatatgggt ccgccgctcg cgtcggagtt cggtacaatc tggtagcatc ccgtcgtggg
     1021 ggaggagccc tcggtgaacc aagatcaatc tgccgatcga aagcaagtag taggaaagct
     1081 acgattaaac cccgtgcgtt acactcctga gccgtggcgt gttttggagc aaacccctgg
     1141 ggacgcttgg atggtagtag gtgaatcttc aacgcatggc gctcgctcgc ttgccaccat
     1201 tcatgacgcc gtctccgact tggagcagca agcgaatgcc cgcctgattg cctcggttcc
     1261 atctatgcac cgcgccgccg taaagctttt gtgtgcctgg ggaacggagg acgctccaga
     1321 ggccatggaa gagctggccg tgatattgag tcaggccgtg tgacgcgaat ctggttggtc
     1381 gctcctgcga caagtggcac cgctaaattt gaggttaaga agactggaag ttcggtatta
     1441 attcacgcgc cgcgaggagt cttgaatgtg agtgcatcag gaaagacttt caccttggcg
     1501 gagggcacga cggtgactct cgatgcggga atcgggaggg ccaaaactgc atcacacagt
     1561 gctgcggccg cgggtctgta tggcgctcag tcatccacat cattctacaa tctcgaaggc
     1621 gttctttctg acccatctga ctcgggtctc ccctggtgta gtaatgtcca cttgtgcatt
     1681 atcccccctt cgacttcggg ttatcatccg tgccgttgcc ggaagctcta ggctcaaatg
     1741 agatttttgc cttgcgcttt taccaaggca ccttactcct gaacgcacga cagcccagct
     1801 aaaatttggc aatttagctg ggctgtggct tttctcttcc gtctgtctag agtttgatcg
     1861 tggcagccag gtcggactta gcttgaccac ctcagaccct tcacgccgtc accggtaata
     1921 gagcctgtga gcgataccac tcgaccgttt tgttcagacc ttcttcgaac tcaaccgatg
     1981 gtcgataacc caggtgctgc ttggctaagc ggatatcggc gagcgagtgc ttgatatcac
     2041 ctgagcgttc cggagcgtaa aaggcggcgt tggcgtagcc tagtagttgc tgcagtaaca
     2101 cgtaggtctc gttgatcgtg acccgccgac ccccagcaat gttaaagtat cgtccggcga
     2161 ccttatcggc aggagcatgg caagccagta ggttcgcctt gacaacattg tcgatatagg
     2221 taaagtcgcg actttgctcg ccgtccccat atatggtggg tcgttcttgc cgcaacatct
     2281 tcggaatgaa gatggccatc acacccgtat acatcgactt tggatcttgg taaggcccga
     2341 agacattgaa atagcgcagg gagactgtct caacaccgta gaccgcatgg aacgatctca
     2401 tgtagtactc acccgccagc tttgacacag catacggtga aatcggattt ggcgtcaggt
     2461 cttcgctctt gagcaaatga ggagtgtcgc cgtacacgga ggacgaggaa gcggatacta
     2521 tccgtctcac tcgggcgtcc cgagcagcca ctagcaggtt caaggtccca tcgacattcg
     2581 agcggttgca ggatagcgga tcggcaatcg agcgcggaac cgaaggcagc gcagcctggt
     2641 ggatgacgta gtcaactccc tggcatgcct cgctgactcc ccgcatatcg aggagatcca
     2701 cttccctgaa gtcgatagcg ctgcttatta aaaccaagtt ttctggtttg ccggtactga
     2761 agttatcgat tccgcggacc ctctctccgc gccgaaccaa ttctcgcgca atggacgagc
     2821 cgatgaatcc ggcaatccca gtgatcagat aggtggacat ggaaatgggg tgattcctcc
     2881 ctactgaaat caatgcactc gatcctgcca ggctagttgg ctgtacggga ttcgcttttg
     2941 gtccggagac gctcagaatg gctgagattc aaattgtaag attgcattgc tcgcttgctt
     3001 tccaaagtct cccaacttct actttggccg gtgaggagtg caacgaccga ccagttggct
     3061 cggaagaacc gctactaaac cagatataca agccagccag cattacaatc aggataaaaa
     3121 acagaatggc tagccgccgc ataacctttt ttctacgctt atggcgtgac ggctggcgct
     3181 taccgctgcg atggctgtgg tgatgatgct ggtgtggaat ctggactgcg gaagtctcca
     3241 tgctgtgctc cataggttgg ccgctcgacg gggactgtcg gtcttgggac aaagtgccta
     3301 ctcactgaac gggctgaaaa ccactggacg ctccgcttgc cccgcatgcc atagctgctc
     3361 agtcattcgc gacagcaatt gcagagatca acgtcagttt cgcctctcgc cctgcaatgt
     3421 gcgatggcaa aacctcaatt gggacggcct cttcaacata gcggtactct ggcacaattt
     3481 gcttcagctt ctcgcgaatc atggtgtccg tactggcata caccataccc tccaattcgc
     3541 gcaggtgctc atggaggaca ggccagctca tcagcttact cgaagtgcag aagatcttct
     3601 cgacgtctgt ccggaatgca ctctcactag cgtaaaaaag ttcctcgtgg agcttctcgc
     3661 ccttacgcaa acctgtgaac acaatttcga cgtcatcttc gcttttcccg cagagtcgaa
     3721 ccagggtacg agctacatcc acaatcttaa tcggtttacc catttcgagg accaggatct
     3781 ctccgtgttt gccaatcgta aacgcctgca gcaccagtga tacagcctca ggaatggtca
     3841 tgaagaagcg ggtgatgtcg ggatgtgtca ctgtcacgat gccttgcttc cggatttgtt
     3901 cctgaaagac cgggattacg ctgccctgag atcctaatac attgccaaat cgaacggaga
     3961 cgcgccgcat cttggagttg ggtttcgccg ccataatcag ctcgcagaca cgcttggtgc
     4021 agcccattac attggtcggg tttaccgcct tgtccgacga gatcatgagg aagtcggcgc
     4081 acccgcattg ctccgccaca tcaagcagag acatcagtgc caagacattg ttccgtactg
     4141 cctcgcttct gttggtctcc atcatcggga catgcttata cgcagccgcg tgaaaaatga
     4201 cgtcgacccc ggcagccgag atgagacctt tcatacgttg ccggtcggcg acatctgcaa
     4261 cacaatattc ggcacatacc tttgcagcgg catcccctat cttctgctca aggaagaaga
     4321 gcgcactctc atcttgatcc agacacacga gtttactggg cttgtagtat aggagctggg
     4381 cgcacaactc agagccgatc gatcccgcag ctcccgtaac cattaccacc ttgccagtca
     4441 attgttggcg aacagcctgt aagtcaaaat gtatgggctc tcgtcccagc aggtcgtcga
     4501 gcttgacatc acgcaactgc tccgcggtaa cttttccgtt gatcaaatcc gacattccgg
     4561 gaatcatgcg gaagcgtgcc cccgactctt cgcacagagc cgtgatacgg cgcatctggc
     4621 gcccggtcgc cgacgggata gcaattagca cctcttccac ggcacgtttt cgtacgagcc
     4681 gtggcagatc atcaatactg ccaataacgg aaacgccgtg tattcgtcct ccaatttttg
     4741 ccggctcgtc gtccaccagt ccgacagcca tatatccgca gcgaggcaag tcattaagca
     4801 gcctttccgc cgcgtccccc gctccgacaa tgatcgcagt cttttctgac ctgcctctcc
     4861 aagcacccct gacggactgc gcgagcattc gcgagaacag gcgagcacca cacaaacccc
     4921 ctacaacaag cagtgcttca agacagtaaa tactaatagg aaatgctttt ataccgagga
     4981 gccatcgctc tatcacgaga aagccgagcg accccaaagc cgtagctttt acgatatctg
     5041 caatatcact gatgccggtg taacgccagt acccgcgaag cagtttaaag cggcccaagg
     5101 ccgccaagcg catcaacaca agtattggca aagcggcaaa cagaatatcg cgctggggca
     5161 gcgtaaactc aaaacgcagg agccaggcgc cgatgagaga tgctgcaaaa agaatcgtgt
     5221 cgaatgcaag tatgaaccaa aaccgtagtc gcaatagcat ggccagattc ctattcttga
     5281 tgggacgtgg ggagcgtgtt gatcagccgt tactgctagc tgatgtgaga aaggaacagc
     5341 ttctaggaag ccctcggctg ggtcttgcgg tgagcagcca ctctcatgac attcttgact
     5401 gtcgcgcaag ttgcgtggat ggcttcctcg gtgagcgtgg gatgcacaag aaacatcaga
     5461 ctagtttcgc ccaattcgtg cgcaaccggt aggggatcct tcggccgcca gtctgctgga
     5521 aatgcctttt cgaggtaaat ctcgctgcag ctgccggtaa aacacggcac tccttctgcg
     5581 tttatggctt cgacgatgcg atcgcggctc cagccaggcc gcaactgttc gggacgcacg
     5641 aaaacatagt atttgtagta agagtggtcg acgtgcacgg gcgggtaggt tacgcgcaaa
     5701 cccggcagct gcgcaaaaca gttactcagc gtccgggcat acttgcgcct gatgcttagc
     5761 cagtgactga ccttgcgaag cactacccgc ccgatcgccg actgcacttc ggtcatccgc
     5821 cagttggttc caaatgactc atgcagccag cggtatccgg gaacatgctc ctttcggtaa
     5881 acggcgtcgt agctcttgcc gtgatctttg taggcccaca tcttctccca aagatcatgc
     5941 gagttggtgg tgaccattcc gccttcgccg gcagtggtca tgatcttgtc ctgacagaat
     6001 gaaaatgctg cgacatcgcc catggagccc accggccgac ccttgtaagt cgccccgtgc
     6061 gcttgggcgc agtcctctac aaccttgaga ccgtgctccg ctgctaattc aaggatcgca
     6121 tccatttcgc agggccagcc cgccaagtgc accggcacga tggccttggt acgcgaggaa
     6181 agcactttcc taatgctatc tgcagtgatg ttctggctgt ggcgatcaac atcggccatg
     6241 acgggttgtg cgcccactgc tactgcgcag ctagctgagg caatgaatgt gcgacttgaa
     6301 gtaatgactt catctcccgg accaacgccc agcgctctta gagcgccttc cagagcaact
     6361 gacccgttgg agactaaaat cgcatgcttg catccgttga gttctgcaaa ttccttctcg
     6421 aacagccgcc cctcttcccc ggtccaatag ttgacgttgc cgctctgcaa cacggcaaca
     6481 gcggcttcaa tctcgtccgg agcgaagtac ggccaaggcg agaagagact tttgtacacc
     6541 gaagccttac ccttcagcgc tggctgatgc tgaacagatt tggaatttgt agccattagg
     6601 acagcagcct tgtttgccac agcttgaaaa gtttactttt tagcaattct ttaatcactc
     6661 cgaaagttct ccggtcactc tgaattgtgt cttgcgagtt tccgctcgat gcaggagtct
     6721 ggccgggatt ccgacaacgg tggtgccgga cgctactgaa tcgaggataa cagcgcccgc
     6781 cccgatgatc gcaccctcgc caatttcaga cccagggagg gcgatcgcgc caatcccgac
     6841 atgtgcaaac gaatgcaccg tcacgccgcc acctag
//
LOCUS       DASRKK010000269         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_996913, whole genome shotgun sequence.
ACCESSION   DASRKK010000269 DASRKK010000000
VERSION     DASRKK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996913"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            671..1216
                     /locus_tag="VFC15_05955"
     CDS             671..1216
                     /locus_tag="VFC15_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09739.1"
                     /translation="MTLRQKEGRAVNAVAELLRKTLSVPNIYLEPRSPKLAVDVLAVD
                     RAGSGDLHGVEIKVRDDLRVKNPSSLKALNQQIKGRSKRNIAEIRRQLMSLPAHYKYL
                     AVSQSDCDLVLTEMEPRLYSPDGIGRIGIISITDQGEEPPRAEIVIAPERFRVDAAKL
                     TKIEKNLINNKKVRPDIEVRI"
     gene            1233..2330
                     /gene="dusB"
                     /locus_tag="VFC15_05960"
     CDS             1233..2330
                     /gene="dusB"
                     /locus_tag="VFC15_05960"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZM09740.1"
                     /translation="MHKYFDNPAERIAPTDACVPASVSLGGVEIRPATVLAPMAGITD
                     TVFRRFIRNLGGCGLMMTEFTSADGIIRHKDKKAKQYLTFYGNEHPISAQLFGSDPAT
                     LAEAARVVEGLGFDLVDLNLGCPAKKVVKCNGGSGLLRDLPLIKNIFESVRAAVKVPF
                     TVKFRAGWSDKEIVHINLAKMAEDCGLAGVALHARTREQGYSGQAQWEWIAAVKDAVK
                     IPVIGNGDVRSPEDAAAMVAQTGCDAVMIGRSAASNPWIFRQIEQFTRTGSYDLPSDA
                     DRYHMIRTYFSMLVEEKVGGAEGKMKQFAAWFTHGVPHGAQLRKAVYESKREIEILDA
                     VDRFFEMLLNGKIPSAAQSVEDEVRTAALPA"
BASE COUNT          590 a          672 c          825 g          466 t
ORIGIN      
        1 ggcaaacaga agctgatgga caccgcgggc cgggtcgagc gcttccgccg caagtacgcc
       61 aaaagcgatg caggcaaagc agaagcagcg aaggccgaag ccgcgaaagt cgaggccgcg
      121 aaagtcgaag ccacgaagaa ctaacgtctg caagtgttta agacagagcc tccgcagcgg
      181 cggaggcttt ttgcgcatac aggtaaaatg ggtcttgacc gttaaccgta tgggtgagat
      241 tagggacagg gtctatcgtg atcgcggatc acaactggca aaagcgatga ggctctgccg
      301 gtacaaccac cctgcttatc cttcggccgc cgccctttta gcggtacaca gcgcgatcgc
      361 gtacaacgac gctgttcaga tcaagttaac gggtgaccgc tggaggagtc aggatcacgg
      421 tcaggcgata gcagcgatta aaagggcgtg cgggacggcc aagattgagc cgcgtggcat
      481 cgcgcatcta gggaaactct tgagcatgaa aacggacatt tcgtacggtg acaaggaagt
      541 ggataaccaa cttgccgagg ctctgtgcgt ggctgcggat cgattcgagg cctgggctga
      601 aggatacgtc ctcaatagga gatgaaaggg gaagcgtatg ccagtgataa gcaagggtaa
      661 gaggcgaacc ttgacgcttc gccagaagga aggacgtgcg gtgaatgccg tggcggaatt
      721 gctgcgaaag acgctgtctg tgcccaatat ctaccttgag ccgcgctccc cgaagctcgc
      781 ggttgacgtg cttgccgtag atcgggcagg ctcgggtgat ttgcacggcg tagagattaa
      841 ggtacgggat gatctcaggg tcaagaaccc ttcgagtttg aaggcattga atcaacagat
      901 aaagggccgg agcaagagaa acatagcgga aatacgaagg cagcttatgt ccttgccagc
      961 gcactataag tatttagccg tgtcgcaaag tgactgcgac ttggtgctaa cggagatgga
     1021 acctcgttta tattctcctg acggaatcgg caggattgga attatctcga ttacggatca
     1081 aggagaagag cctccgaggg ccgagatcgt gatcgcgccc gagcgttttc gggttgacgc
     1141 cgctaaactc accaagatcg agaagaactt gatcaacaac aaaaaggtac gtccggacat
     1201 agaagtacga atctgaatca gctgattagc taatgcacaa gtatttcgac aatccggctg
     1261 agcgcatcgc gccgactgac gcgtgcgtgc ccgcgtcggt gtcgctgggt ggagtcgaaa
     1321 tccggcccgc gaccgtgctg gcgcccatgg ccggcatcac cgacacggtg tttcggcggt
     1381 tcatccgcaa cctgggcggc tgcggcctga tgatgaccga gttcacctcg gcggacggca
     1441 tcatccgcca caaagacaag aaggccaagc agtaccttac gttctacggg aatgagcacc
     1501 cgatttcggc gcaactgttc ggcagcgatc cggcgacgct ggccgaggca gcgcgcgtcg
     1561 tcgagggcct gggcttcgat ctggtcgacc tcaacctcgg ctgtccggcg aagaaagtgg
     1621 tgaagtgcaa tggcggctcg ggactgctgc gcgatctgcc gctcatcaag aacatcttcg
     1681 agtcggtgcg cgcggcggtg aaagtgccat tcacggtaaa gttccgcgcg ggttggagcg
     1741 ataaggaaat cgtacatatc aacctggcga agatggcgga ggactgcgga ctggccgggg
     1801 tcgcgctgca cgctcgcact cgtgagcagg gttacagcgg gcaggcgcag tgggagtgga
     1861 ttgcggcggt gaaggacgca gtcaagattc cggtgatcgg caatggcgac gtgcgctctc
     1921 cggaggacgc cgccgccatg gtggcgcaga ccggatgcga cgcggtgatg atcgggcgca
     1981 gcgcggcgtc gaatccgtgg atcttccgcc agatcgagca attcacccgc accggatcgt
     2041 atgacctgcc tagcgatgcc gaccgctacc acatgatccg cacctacttc agcatgctgg
     2101 tggaagagaa agttggcggc gcagaaggca agatgaagca gttcgcagcg tggttcaccc
     2161 acggcgtgcc gcatggtgcc cagttgcgca aagcggtgta tgagtccaag cgcgagatcg
     2221 agattctcga cgcggtggat cgtttcttcg aaatgctgct caacgggaaa attccgagcg
     2281 cggcgcagag cgttgaggac gaagtacgca ctgcagcgct tccggcgtaa gcggtttggc
     2341 ccaccagcac cagcaccatc ctgtacaatt ccggaacctc atccgacaca cggacgtacc
     2401 acctcgtgat tcctggagga catcctgcgc aataaatgga aagaagtggg cctcggcctg
     2461 ctgaacctgg tggccgtcgt tctggtcata gcgctgttcc agccggtgct gcggaagcac
     2521 atgggctcgg cgggagcggc cgtgctggca gtg
//
LOCUS       DASRKK010000270         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_214661, whole genome shotgun sequence.
ACCESSION   DASRKK010000270 DASRKK010000000
VERSION     DASRKK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214661"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFC15_05965"
     CDS             <1..420
                     /locus_tag="VFC15_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZM09741.1"
                     /translation="EELFIMDMGLSQNPLDVAYATRTIPLGQFWITGGAALPTGRTAI
                     ENALDRLNKQRLLADGKFTDPHKATLVFVRTLLQDGASQHIAYETAENHRRPRPHSKP
                     DGADAPEQRVSAYTPGPNSRCPCGSGKRYKRCCGVTA"
     gene            complement(1083..>2886)
                     /locus_tag="VFC15_05970"
     CDS             complement(1083..>2886)
                     /locus_tag="VFC15_05970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM09742.1"
                     /translation="RDGGSQIWVQDFDSVSGTLTGEPRKVTSISTEASGGLWSPDGKS
                     ILFVSEAYPDCKDDACNKQRDEEQAKSKVKAKVFTQLMFRHWNHYFEGKYSHLFVVSS
                     DGGVARDLTPGAHDVPPFSLGGQDQYQFSPDGQEIAFTSNIDEVQATSTNSDIFLVPT
                     AGGAPKKITTNPGSDDTPMYSPDGKYIVYRSQLRGGYESDRFRLMLYERAAGKIGDLA
                     PNFDRWVDSMAWAPDSKTIYFTAENEGEAPIYAINVTDPKPWPQELVAGFNDSPTPTP
                     DGKTLLFDRMSVAAPNEIYSAPLLFPQCRMAVPPNAYSIPDCLLSKAQAVTNLNGSVL
                     SQVEMQPLENFWFTGAAKEKVQGFMVKPPNFDANKKYPVKFLIHGGPEGAWGDDWSYR
                     WNPELFASNGYVVIMVNFHGSTGYGQKFIDAINGDWGGAPFHDLMMGLDYAEKTYPFV
                     DKDRECALGASYGGYAINWIEGHTTRFKCLVSHDGMFNTESAFGTTEELWFNEWEFKG
                     TPWTKREMYRKWSPHLSATNFKTPILVVHGQLDYRLDVSEGFQLFTTVQQLKIPSKML
                     YFPDEGHWVLKPQNSRLWYQTVTDWVDQWTKPAK"
BASE COUNT          576 a          861 c          812 g          637 t
ORIGIN      
        1 gaggaacttt tcatcatgga catgggtctc agccaaaacc cactcgatgt cgcctacgcc
       61 acccgcacca tccccctagg ccagttctgg atcaccggtg gggctgcact tcccaccggc
      121 cgtacggcca tagagaatgc acttgaccgt ttgaacaaac agcggttgct ggcggacggc
      181 aagttcactg atccgcacaa ggcaacgttg gtcttcgttc ggacgctgct tcaagatggg
      241 gcttcccagc acatcgcgta tgagaccgcg gagaatcatc ggcggccccg gcctcatagc
      301 aaacctgatg gagcggatgc gccggagcag agagtgtcgg cgtacacccc aggtcctaac
      361 tcccgctgcc cctgcggcag cgggaagaga tacaaaaggt gctgcggtgt aacagcctga
      421 acccggccaa gaatttatgc agccttgccg aaaacacacg atagacctgc cgctactgct
      481 cttaaccgat gtggtgatca aatcgaaccc aagttcccga acatcccgtg ctgcgcgggg
      541 cggatggtcg taacctgctg ccttaatctc ctcggtggtt acagcacctt tcttggcgat
      601 ggtgtccagg acgaatctag cgcgcttgtt tctaacggcc tttattttgt ggagcaggtc
      661 tcgcggaagt tttcccacgt ggttcgctct cggaaatgag ttctgccgcc gagactccta
      721 acgcagagga aatatcgagc agagtgttga gcgtcacatt ccgctcgccg cgctcaattg
      781 cgccgatgta agtcctatgc aaattacact ctgctccaaa ttgctcttgc gaccacccac
      841 gtgctgtacg cagccgcttg agattcttcg cgagtatctc attaggattt ctcgatggca
      901 tccagagcag tgtttctgca tgatgactat ttatctacag actagatata gcaataacaa
      961 tcgaccccag cggcacaggt gaacaacccc gatatttcac cagactgtcg gcagatcgtt
     1021 gcacaggtgc gcaaatagtg cggcgccctt ccccgctctt taggcaccgc catcgcccga
     1081 atctacttcg caggcttcgt ccactgatcc acccaatcgg tcaccgtctg gtaccagagc
     1141 ctgctgttct gcggcttcag cacccagtgg ccttcgtcgg ggaagtagag catcttcgac
     1201 ggaatcttca gctgctgcac cgtggtgaac aactggaagc cctccgagac atcgagccgg
     1261 taatcgagct gcccatgcac caccaggatc ggcgtcttga agtttgtcgc cgacagatgc
     1321 ggcgaccact tgcggtacat ctcgcgcttg gtccacggag tgcctttgaa ctcccactcg
     1381 ttgaaccaca gctcctcggt cgtgccgaag gctgactctg tgttaaacat accgtcgtgc
     1441 gagaccaggc atttgaagcg ggtggtgtgg ccttcgatcc agttgatggc gtatccgcca
     1501 tagctggcgc ccagggcgca ctcgcggtcc ttgtcaacga acggataggt tttctctgcg
     1561 tagtcgaggc ccatcattag gtcgtggaag ggagcgccgc cccagtcgcc gttgatcgcg
     1621 tcaatgaact tttgcccgta gccggtcgaa ccgtggaagt tgaccatgat cacgacgtaa
     1681 ccgttggagg cgaacagctc gggattccag cgatacgacc agtcgtcgcc ccacgcgcct
     1741 tctggaccgc cgtggatgag gaacttcacc ggatacttct tgttggcgtc gaagttgggc
     1801 ggcttcacca tgaagccctg gactttctcc ttcgccgcgc cggtgaacca gaaattctcc
     1861 agcggctgca tctcaacctg cgagagtacg gacccattca gatttgttac tgcttgcgcc
     1921 tttgacagca gacaatccgg gatactgtat gcattcggag gcacagccat tcggcactgt
     1981 gggaacagca gcggcgcact ataaatctca ttcggcgcgg caacggacat gcgatcgaag
     2041 agcagagtct tgccgtccgg cgtaggcgtg gggctgtcgt tgaagccagc gaccaactcc
     2101 tgcggccacg gtttcgggtc ggtcacgttg atggcgtaga tcggagcttc cccttcgttt
     2161 tcggcggtga agtagatggt cttcgagtcc ggcgcccacg ccatcgaatc gacccagcgg
     2221 tcgaagtttg gcgccaggtc gccgatcttg ccggcggcgc gctcgtacag catcagccgg
     2281 aagcggtcgc tctcgtagcc gccacgcagt tgcgagcgat acacgatgta cttgccatcc
     2341 ggcgaataca ttggcgtgtc gtcgctgccg ggattggtgg tgatcttctt cggcgctccg
     2401 cccgcggtcg gcactaggaa gatatcgctg ttagtgcttg tggcttgcac ttcatcgatg
     2461 ttgctggtga aggcaatctc ctgcccatcc ggagaaaatt gatactggtc ctgcccgccg
     2521 aggctgaacg gtggcacgtc gtgcgcgccg ggggtcaagt cgcgcgccac gcctccatcg
     2581 gacgagacca cgaacaagtg gctgtacttg ccttcgaagt agtggttcca gtggcgaaac
     2641 atcagttggg taaagacctt ggccttcacc tttgacttgg cttgttcctc gtcgcgctgt
     2701 ttgttacacg cgtcgtcctt gcaatcggga taggcctccg agacgaacag gatgcttttg
     2761 ccgtcgggcg accacagtcc gcctgaagcc tcggtcgaga tactagtcac cttgcgcggt
     2821 tcgccggtca acgtcccgct gacggaatca aagtcctgaa cccaaatctg tgaaccgcca
     2881 tcgcgt
//
LOCUS       DASRKK010000271         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_899466, whole genome shotgun sequence.
ACCESSION   DASRKK010000271 DASRKK010000000
VERSION     DASRKK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899466"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..897
                     /locus_tag="VFC15_05975"
     CDS             157..897
                     /locus_tag="VFC15_05975"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZM09743.1"
                     /translation="MKKPESKLQELVTRLQQACGENLVSIVLYGSAAREDFHEQFSDV
                     NVLVVLQHLQAASFAAISAVLHWWSREEKLRPPMIMTLEELRESADVFAIELLDIQRS
                     HKTLFGQDVVTAIDVPMNLHRVEVEHELRTTLLRLRHHLLLSPDNQDELRAVLAKSIT
                     SVLTLFRHALIAVGENPPQAKPQLLESAGEVFGFEVQPLRSILELRNEGAHSANVHEL
                     YHAYMSAIQRVAHELDARVPKRQLQKLP"
     gene            942..1541
                     /locus_tag="VFC15_05980"
     CDS             942..1541
                     /locus_tag="VFC15_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZM09744.1"
                     /translation="MKKGIIVLVVIVLLVLWIGGTFVGHRNEMVRKRETVNAAWAQVD
                     VVLQRRADLIPNLVETVKGFAIHEEKVFGDIAAARAAMAGARTPQEKIAANGQLDSAL
                     SRLLVVVENYPQLRSNENFLRLQDELAGTENRIAVERRRYNETVQDYNTYISLFPNNI
                     VASLSGFTRNDAYFKTEEGARQAPKVNFGAQQPAPAPAK"
     gene            complement(1749..>2876)
                     /locus_tag="VFC15_05985"
     CDS             complement(1749..>2876)
                     /locus_tag="VFC15_05985"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HZM09745.1"
                     /translation="PIYQTATFEQEEADSFGRYDYSRSGNPTRTVLEDLLARLENADR
                     AFCFSSGLAAISAVARLLSVGDEILAGDDLYGGTYRLFSKILHRTGVTVRDADACNIE
                     TFAAQITPKTRLLYIETPTNPLLRIVDIRKLADLAHQHGALLCVDSSAMSPYLQNPLD
                     LGADIVVHSATKYLCGHSDVTAGVVAVKRPELAEQIYFIQNGEGSALGPFDCYLLLRG
                     VKTLKLRLDCQQANALKVANFLSVHPAVRRVHYPGLASHPGHDVHTKQARGAGAVISF
                     ETGSFEVSKQVVEATQLFRISVSFGSVNSSISLPGCMSHASIPAEVRQARSLATDLVR
                     LSVGIEDVDDLLADLERAFERAACAVAVVKDGAAVREQQFI"
BASE COUNT          607 a          822 c          885 g          562 t
ORIGIN      
        1 gccgtggagg tagctggggc ggcgggtttg gcggcagttc cggaggctac tcgggcggcg
       61 gcggtggctt tggaggcttc ggcggctttg gcggggggat gagcggcggt ggtggcgctg
      121 gcggcagttg gtgagccacg ccgggagtga cgatcaatga agaaaccaga aagtaaactg
      181 caagaactcg ttacccgatt gcagcaggcg tgcggcgaga atctggtgtc tatcgtgctg
      241 tatggttccg cggcccgcga agacttccac gagcagttct ccgacgtgaa cgtgctggtt
      301 gtgttgcagc acttgcaggc ggcttcgttt gcggcgatct cggcggtcct acactggtgg
      361 agtcgggaag agaagctgcg gccaccgatg atcatgacgc tcgaagagct gcgcgagtcc
      421 gccgatgtct tcgccatcga actgctcgat attcagcgct cccacaaaac gctgttcggc
      481 caggatgtag tgacggcaat cgatgtaccg atgaacctgc accgggtcga ggtagaacac
      541 gaattgcgca cgacactgct gcggttgcgc caccacttgc tgctcagtcc tgacaaccaa
      601 gacgaactcc gcgcggtgct ggcgaaatcg atcactagcg ttctgacgct gttccgccat
      661 gcgttaatcg cagtgggaga aaatccgcca caggcgaagc cgcagttgct ggaaagtgcc
      721 ggggaggtct tcggatttga agtgcagccg ctgcggtcaa tcctggagtt gcggaacgaa
      781 ggcgcgcatt cggcgaacgt gcacgaactg taccatgcgt acatgagcgc aattcagcgg
      841 gtcgctcacg aactggatgc gcgcgtaccc aagcgtcagt tgcagaaatt gccctaggga
      901 gacgtcatct gtccatctag aatcggcggg gagagattgg catgaaaaaa ggaatcatcg
      961 tattggtcgt gattgtgttg ctggtgctct ggatcggcgg cacgtttgtt ggccaccgta
     1021 acgagatggt ccgcaaacgc gaaaccgtga atgcagcctg ggcgcaggtg gatgtcgttc
     1081 tgcagcgccg ggccgacctc atcccgaacc tggtcgagac ggtgaagggg tttgccatcc
     1141 acgaggaaaa ggttttcggc gacattgcgg cagcgcgcgc cgcgatggcg ggagcgagga
     1201 cgccgcagga gaagatcgcc gccaatggtc agctcgattc cgctcttagc cgcttgttgg
     1261 tggtcgtgga gaattacccg caactccggt ccaatgaaaa ctttctgcgc ttgcaggacg
     1321 agctagcggg cacggaaaac cggatcgcgg tcgaacgccg ccgttacaac gagaccgtgc
     1381 aggactacaa cacctacatc agcttgttcc cgaacaatat tgtcgcaagt ctttcaggat
     1441 ttacccgcaa cgatgcctat ttcaagacgg aagagggagc acgtcaggcg cccaaggtga
     1501 atttcggcgc gcagcaaccg gctccagcac cggcgaaata gagttagtgg aattcaggaa
     1561 gggcgggcgt tcagctcgtc aatgttgtca tcacgagcgg gctttcgccc gcgagggacc
     1621 tgcttgtgca tggctatgga aaaagcagat tcctcgcgcc aaaatcgggc gctcgtaatg
     1681 acaattcata aatcacgcct ttcgcccacc aatgtgtcac cttaattcgc gatcctcaat
     1741 cacgggcgtt aaatgaattg ctgctcgcga accgccgcgc catcttttac gacggcaact
     1801 gcgcacgcgg cacgttcgaa cgcccgctcc aagtcggcga gaaggtcgtc cacgtcttca
     1861 attcccacgg acaggcgaac caggtctgtc gctaacgagc gcgcctgacg gacctccgcc
     1921 ggaatactcg catgtgacat gcatccgggc aggctgatcg aggagttcac gctgccgaag
     1981 cttacgctga tgcggaacaa ctgcgtcgcc tccacgactt gcttcgacac ttcgaacgag
     2041 ccggtctcaa agctgatgac cgcccccgct ccacgcgcct gcttggtatg cacatcgtga
     2101 ccgggatggc tggccagccc ggggtaatgc acgcgccgca ctgcaggatg cacgctcagg
     2161 aagttcgcca ctttgagcgc attcgcctgc tggcaatcga ggcgcagttt cagagttttc
     2221 actcctcgca gcagcagata acagtcgaag ggaccaagcg cggaaccttc cccgttctga
     2281 ataaaataga tttgctccgc cagttcgggc cgctttactg ccaccactcc cgccgtgacg
     2341 tcgctatggc cgcaaagata cttggtcgcc gagtgcacca cgatgtcggc ccccagatcg
     2401 agcggattct gcaagtacgg cgacatcgcg ctgctatcca cgcacaacag cgcgccatgc
     2461 tgatgcgcca ggtcagccag cttccggata tcaacgattc gcaacagcgg gttggtcggg
     2521 gtctcgatgt aaagcagtcg agttttcgga gtgatctgcg ccgcgaaagt ctcgatgttg
     2581 cacgcatccg catcgcggac ggtcacgcca gtacgatgca ggatcttgga gaagagccgg
     2641 taggttccac cgtacagatc atctccggcg agaatctcgt ctcccaccga gagtagtcgc
     2701 gcgaccgcac tgatcgccgc caaaccgctg gaaaaacaga aagcgcggtc ggcattctcc
     2761 agtcgcgcca agaggtcctc aagcacggtg cgggttggat tcccgctgcg cgagtaatcg
     2821 tagcggccga agctgtccgc ctcttcctgt tcgaaagttg cggtctggta gatcgg
//
LOCUS       DASRKK010000272         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_20361, whole genome shotgun sequence.
ACCESSION   DASRKK010000272 DASRKK010000000
VERSION     DASRKK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20361"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VFC15_05990"
     CDS             <1..1391
                     /locus_tag="VFC15_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZM09746.1"
                     /translation="LYRKAGPNEVLVVYGFRGTRVVKGHGTVIFPMVETYRELSLELM
                     SFDVAPQQDLYTRQGVAVTVEAVAQIKVKSDPESILTASEQFLTKAPQDREALIRLVM
                     EGHLRGIIGQLAVEEIVKQPEMVADRMRSTCADDMNKMGLEVISFTIKEVRDKNEYIA
                     NMGRPDIARIKRDADVATAEAERDTAIRRAIALRESAIAKAQADQERVAAETASQARQ
                     AEAQRDLEVKRATYTELVKRQQAQADKAYEIQTNIMQQQVTAEAVKVQQVEKEQQVKV
                     QEAEILRREKELIATVLKPAEIERQRIETLAGAEKQRLTVEAEGHASAIRAQGEAEAE
                     IIFKKGDAEARAMNVKAAAYQEYNQAAVVDKLFTNMPEIVKALASPLAKVDRITVVST
                     GDGNSAGLHKITGDIAQMAAQVPALFEALSGMKMSELLGKVRTLGDKTNRSDELPPSE
                     GDGKSKAAGTGK"
     gene            1636..>2251
                     /locus_tag="VFC15_05995"
     CDS             1636..>2251
                     /locus_tag="VFC15_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZM09747.1"
                     /translation="MALLDRVATLVRANLNDLVDRAENPEKMLKQVILDMENQFMQVK
                     TQVAIALADLHLLEKRKKEHADKHAEWMRKAELAVDKKDDELARAALERAMSFQQLTD
                     SFEKQIADQEVQVEALKSALKKLELKLSEARGKADLLIAQHRRSRAVNRAADAQTTPT
                     GENRTFERMRSKVAREEALGQAKGEVLGDDIDGRLDALEREQKID"
BASE COUNT          560 a          630 c          721 g          340 t
ORIGIN      
        1 gactctaccg caaggcaggg ccaaacgagg tcctggtggt ctacggattt cgcggcactc
       61 gcgtggtgaa gggacacggc acggtcatct tccccatggt tgagacctac cgcgagctat
      121 cgctggagtt gatgtccttc gacgttgcgc cgcaacaaga cctctacacc aggcagggcg
      181 ttgctgtcac cgtcgaggcg gtggcgcaga tcaaggtgaa atccgatccg gaatccatct
      241 tgaccgcgtc cgagcagttt ctcaccaagg cgccgcagga ccgggaagcg ctgatccggc
      301 tggtgatgga agggcacctg cgcggcatca tcgggcagct cgcggtcgaa gagatcgtga
      361 agcaaccaga gatggtggcc gaccggatgc gctccacctg tgccgatgac atgaacaaga
      421 tggggttgga ggtgatctcg ttcaccatca aagaagtccg cgacaagaac gagtacatcg
      481 ccaacatggg gcgtccggac atcgcacgca tcaagcggga tgccgacgtc gccaccgccg
      541 aagcggaacg cgacaccgcg atcaggcgtg ccatagcgtt acgcgaatcc gccatcgcca
      601 aagcgcaagc cgaccaggag cgcgtagccg ccgagacggc ctcccaggcc cggcaagcgg
      661 aggcacaacg cgatctcgag gtgaagaggg caacctacac cgagttggtg aagcgccagc
      721 aggcgcaggc cgacaaggcg tatgagattc agaccaacat aatgcagcag caggtgaccg
      781 ccgaagccgt caaggtgcag caagtcgaga aggaacagca ggtgaaggtg caagaggccg
      841 agatcctgcg ccgcgagaag gaactgatcg ccaccgtact gaagccggcc gagatcgagc
      901 gccagagaat cgagacgttg gccggcgccg aaaagcagcg gctgacagtc gaagcggaag
      961 gacatgccag cgcgattcgg gcgcagggcg aggcggaagc cgagatcatc ttcaagaagg
     1021 gcgacgccga agcgcgagcg atgaacgtga aggccgcagc gtaccaggag tacaaccagg
     1081 ccgcggtcgt ggacaagctc ttcaccaaca tgcctgaaat tgtgaaggcg ctggccagcc
     1141 cgctcgccaa agtggatcgc attaccgtgg tttccactgg cgacggcaac tcggccggtt
     1201 tgcacaagat caccggggac attgcgcaga tggccgcgca ggtaccggcg ctgttcgagg
     1261 cgctctcagg catgaagatg tcggagttgc tgggcaaggt aaggacgctc ggcgacaaaa
     1321 cgaatcggtc ggacgaactg ccgccaagcg aaggggacgg caagtccaaa gcggcaggga
     1381 caggtaagtg aagtgaagtg tcaaaagaca cccagcctct tgaattgtca ttacgagcga
     1441 agcgaggaat ctgctgttgt gttgtgctga gaagaacagc ttgagaaaca gcagatccct
     1501 cacggcttaa agccgttcgt gatgacaact cagaaatcgc gaagccgggc cttacgtatg
     1561 cagcactctt tagcccatct cgaaaactat cgtgcgcgct gaacgcacat catcacattt
     1621 cagcaaggag cagttatggc attactcgat cgtgtagcaa cactggtgcg ggccaacctc
     1681 aacgatcttg ttgatcgggc ggagaatccc gagaagatgt tgaagcaggt cattctggac
     1741 atggaaaacc agttcatgca ggtgaagacg caagtggcca tcgccctggc cgaccttcat
     1801 ctgctggaaa agcggaagaa agaacacgcc gacaagcacg ccgaatggat gcgcaaggca
     1861 gaacttgcgg tcgacaagaa agacgacgag cttgcccgcg cggcattgga gcgtgctatg
     1921 agcttccagc agctcaccga cagcttcgag aagcagatcg ccgaccagga ggtccaggtc
     1981 gaagctctga agtcggcact caagaagctg gagttgaagc tctccgaggc ccgtggcaag
     2041 gctgatctgc tgatcgcgca gcatcgccgg tcgagggccg tcaatcgcgc cgccgatgcc
     2101 caaaccaccc ccacgggaga gaaccgcacc ttcgaacgca tgcggtcgaa agtcgcccgt
     2161 gaagaggctt tgggccaggc gaaaggggag gtgctgggcg acgacatcga tggccggctt
     2221 gacgccctgg agcgcgagca gaaaatcgac t
//
LOCUS       DASRKK010000273         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_899847, whole genome shotgun sequence.
ACCESSION   DASRKK010000273 DASRKK010000000
VERSION     DASRKK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899847"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VFC15_06000"
     CDS             <1..1524
                     /locus_tag="VFC15_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZM09748.1"
                     /translation="LQDLVNILRTLLDTQRLQQFPSQQAIVVRGTPDQIALAEKLIDD
                     LDKSRPEVVVDVVIMQVQRNKLRNLGITPPQNVSVALVDNTSTTTSTSGSNVGTGNGS
                     VSTTTSNSTGTINLNSLAHLNATNFQVTIPPATATFLYTDSNTRILQQPQIRALDGQK
                     ASLKIGQRVPVATGSFQPGIGGVGINPLVNTQFNYIDVGVNIDVTPHVHGTDQVTLKL
                     SMDISAVDSYQNIGGIQQPVIGQRKIEHEIRMREGEANIMGGILEETESKSINGIPGL
                     ASIPFFRYFFSTESKQRQDNELVFMLIPHIVRAQDVFDSNTRAIDVGTANSISLHRSN
                     APAADQPANQPGRPGTSALPQAANPPADQTASAPPGSALVSFDPPSLSQAVGSTFAVN
                     INLSGGQNVYSVPLQILYNPKVLQLLNVSNGPLLQQDGQAVALVHRDDSMMGILQVTA
                     SRPPGSNGITADGQVFTLTFQAKAPGQATLNINRATLKNGSMQNIPAGGSQAIVTVH"
     gene            1716..2168
                     /locus_tag="VFC15_06005"
     CDS             1716..2168
                     /locus_tag="VFC15_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZM09749.1"
                     /translation="MRGRRRNGFTVVELIVAMTILMILTAAAVPIVRVTIQRNKERDL
                     RHSLWEMRDAIDRYKDAADRGAFQVKVDSQGYPPDLETLVKGVDSNGKKMRFLRRIPV
                     DPMTGKTEWGLRSMQDDPDSDSWGGQNVFDVYTKSEGTALDGSKYKTW"
     gene            2198..2596
                     /locus_tag="VFC15_06010"
     CDS             2198..2596
                     /locus_tag="VFC15_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZM09750.1"
                     /translation="MNRRSRQRGFTLIELMVVISIIIILMSVAIPRYQVSILRARETV
                     LRDDLYTLRSVIDQYTLDKQKAPQSLQEVVDAGYLKQLPKDPFTNSRETWVPVTDDSI
                     MSPDQSQPGIIDVHSGSDQNSSEGTAYSTW"
     gene            complement(2627..3076)
                     /gene="tsaA"
                     /locus_tag="VFC15_06015"
     CDS             complement(2627..3076)
                     /gene="tsaA"
                     /locus_tag="VFC15_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZM09751.1"
                     /translation="MFTPQPIGFVRTPYSETSQVPKGLGAKHEAEGDLEILPEFEAGL
                     TDIEGFSHLIVIWAFDRSEGFELYGKTPTDGLPHGVFATRSPRRPNPIALTIVELRGR
                     NGPRLHVRGVDMLDGTAILDIKPYLSNVPEEKLRRGWLAEAETRKAR"
     gene            complement(3130..5151)
                     /gene="argS"
                     /locus_tag="VFC15_06020"
     CDS             complement(3130..5151)
                     /gene="argS"
                     /locus_tag="VFC15_06020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZM09752.1"
                     /translation="MYLERQKQLANHVREFLRQKYQIDLPNIAIDQPPKIEMGEFAMP
                     FPFELAKQLRKAPRKIADEIVPEMPLPDGFAKFEVAGAGYINARLVRDQAAGALARET
                     AIAVTDHAAEGAANVSGKILVEHTSINPNKAAHIGHLRNAILGDTFVRLLRAAGHTVD
                     IQNYIDNTGVQVADVVVGFVRLAKLSKAEIEALIASTPRFDYYCWDLYASVSHWYGQD
                     KENLKARLEILHAIEQGNNEMAEIAEMVSTAVLRRHLETMDRLDIEYDFLPRESEILR
                     LHFWDLAFQQLKEKGVLYFETEGKNKGCWVMTRPATQTKVNASRGSDAESSDAEAKTG
                     PDEDAKVIVRSNGTVGYVGKDIAYHLWKFGLLGRDFGYQKFFRYPKGHECWISAEDGE
                     ADHPHFGSVAAIYNVIDSRQSDPQNTVVEAIRLLGYPEKAAHYTHFSYEMVALTPRCA
                     MDLGYEVSAEDQAKSYIEVSGRKGFGVKADDLLDALIAAAKTEVDSRHPELDDTERLQ
                     ISKQIAIGALRYFMLKFTKQSVIAFDFKEALAFEGETGPYTQYAVVRASNIFRKAEVE
                     PEQALSADVDYARFFAEDSGTEIWELWLLAGKTSSVIEQCIATTEPAYAAKHAFQLAQ
                     KFNNFYHRHHILNETDEARKKFLLATAAVVRRELIALLAAMGITVPSVM"
     gene            5409..>5871
                     /gene="larC"
                     /locus_tag="VFC15_06025"
     CDS             5409..>5871
                     /gene="larC"
                     /locus_tag="VFC15_06025"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HZM09753.1"
                     /translation="MFLGALVDAGVPLELFQETVAALHLGITLEVSRVDRNGISATKV
                     DVIVNGQKDLPREEFWAQNEHLSSGRARESAADHAHDHGHTHADEHVHAHGRNLNTIL
                     GIIDSAPLSRRAKLTASEIFVALGAAEAKVHNVDIADIHFHEVGAADAIADI"
BASE COUNT         1248 a         1715 c         1654 g         1254 t
ORIGIN      
        1 ctgcaggacc tggtcaacat acttcgcacc ctgctcgaca cccagcgctt gcagcaattc
       61 ccttctcaac aggcaatcgt ggtccgcgga actcctgatc agatcgctct cgcggagaag
      121 ctgatcgacg atctcgacaa gtctcgccca gaagtggtgg ttgatgtcgt catcatgcag
      181 gtgcaacgca acaagttgag aaacctgggg atcacgcctc cgcaaaatgt gtcggtggcg
      241 ctggtggaca acaccagcac gacgacctcc acttctggtt ccaacgtggg taccggcaac
      301 ggctcggtct cgacaaccac gagcaactct acaggcacca tcaacctgaa cagcctggct
      361 catctgaacg cgactaactt ccaggtgacg atacctcctg cgacggcgac gttcctttac
      421 accgacagca ataccagaat tctgcagcag ccgcagattc gcgcgctcga cggacaaaag
      481 gcttcgctga agattggcca gcgggttcct gtggccaccg gttcgtttca gcccggaatt
      541 ggcggcgtag gcatcaatcc cctggtcaac acccagttca attacattga cgtcggcgtg
      601 aacattgatg tcactcccca tgtgcacggc accgaccagg tcaccctgaa attgtcgatg
      661 gacatctcag ccgttgattc ctaccagaat attggcggca tccagcagcc tgtcatcggg
      721 cagcgcaaga tcgagcacga gattcgcatg cgtgaaggcg aagccaacat catgggcggg
      781 atactcgagg agacggaaag caagtcgatc aacggaatac cggggctggc atcgatcccg
      841 tttttccgct acttcttctc cactgagagc aagcagcgtc aagacaacga actggtgttc
      901 atgctgattc cgcacatcgt gcgagcgcag gacgttttcg atagcaatac ccgtgccatc
      961 gatgtcggaa ccgcaaattc gatttcgctg catcgctcaa acgccccggc ggcggatcag
     1021 cccgcgaatc aacccggtag gccgggaacg tcggccctgc ctcaggctgc caatcctccg
     1081 gcagaccaga cagccagcgc accgccgggc agcgcactgg tgagcttcga tcctccctcg
     1141 ttgagccagg cagtcggatc gacgtttgcg gtcaacatca atctgtcggg cggacaaaac
     1201 gtgtattcgg tgccgctgca gattctgtat aacccgaagg tcctgcaatt gctgaacgtg
     1261 tccaacggtc cattgctgca gcaagacggt caggccgtcg ccctggttca ccgtgacgac
     1321 agcatgatgg ggattttgca ggtcacagct tcgcggcctc cgggaagcaa cggcatcacc
     1381 gccgatggtc aagtgttcac gctgacgttc caggcaaagg ctccggggca ggcgacgctg
     1441 aacatcaatc gcgccaccct gaagaacggc agcatgcaga acatcccggc gggaggatca
     1501 caagcgatcg tgacggttca ctagcacggt cgtgcaagtg aaacctggct ctccgtatga
     1561 ctggaagcgc ttcagacctt gctccgcatc aagtcaagca tctgccggga aggattagaa
     1621 ttcgatcagt gggaaccact ctctgcagta tggttgtgac acttcaatgg gatcctcggc
     1681 aagcgatagg acatcctcat cgagggcttc cggcgttgcg aggacgtcgc cgaaatggct
     1741 ttaccgtggt cgagctgatc gtcgccatga cgatcctgat gatcctcact gctgccgcgg
     1801 tcccgatcgt cagggtaacg attcagcgca acaaggagcg cgatcttagg cactctctat
     1861 gggagatgcg cgacgctatt gatcgctaca aagatgcggc tgaccgcggc gcgtttcagg
     1921 tcaaggtcga cagccagggt tatccgccgg atctcgagac cctcgtcaaa ggggtggatt
     1981 ccaatgggaa aaagatgcga tttctacggc ggattcccgt cgatcccatg accggcaaga
     2041 ccgagtgggg actgcgctcg atgcaggacg atcctgattc tgattcttgg ggcgggcaaa
     2101 acgtctttga cgtgtacacc aagtcagagg gaactgctct cgacggttcg aaatataaga
     2161 cgtggtagct ttgttggcag aacgagaaat tgcgatcatg aaccggcgct cacgacaacg
     2221 cggcttcacc ctgatcgaac tgatggtggt gatcagcatc atcatcatcc tgatgtcggt
     2281 tgccattccc cgttaccagg tgtcgatcct gcgggcgcgc gaaaccgtac tgcgtgatga
     2341 cctctacact cttcgctccg ttatcgacca gtacacgctg gataaacaaa aggcccccca
     2401 gtctttgcaa gaagtggttg acgccggtta tttgaagcaa cttcccaagg accctttcac
     2461 caactccaga gaaacctggg tgcccgtaac cgatgacagc atcatgtcgc ctgatcagtc
     2521 gcagcccgga ataatcgacg tccacagcgg cagcgaccaa aactccagcg aaggtacggc
     2581 ttacagcact tggtgacgga acgcgggcgc ccgcgatagg cgcgggtcac ctcgcttttc
     2641 gtgtctcggc ctcagccagc cagccacggc gtaacttctc ctctggaaca ttcgacaaat
     2701 acggcttgat gtccaggatg gccgtgccat cgagcatgtc aacgcctcta acatgcagcc
     2761 gtggtccgtt gcgaccccgc aactcgacaa tcgtcagcgc aatcgggttg ggacgtcgcg
     2821 gagagcgggt ggcaaacacg ccgtgtggca gcccatcggt aggcgttttt ccgtacaact
     2881 cgaatccctc ggaacgatcg aaagcccaga taacgatgag gtgggaaaac ccctcgatgt
     2941 cagtcaatcc agcttcgaac tccggcaaga tctcgaggtc tccttcagct tcatgttttg
     3001 cgcccaggcc tttggggacc tgactggtct cggagtaggg agtgcgcacg aatccaatcg
     3061 gctgcggcgt gaacatagca tacggccctc aggcgccatc ttacgccggc gcatgcgtgg
     3121 aacaaccgct cacatcaccg aagggacagt gatgcccatt gctgcaagta gagcgatcaa
     3181 ttcgcggcga actacggccg ctgtggccag gagaaacttc tttcgcgctt cgtcagtttc
     3241 gttgaggata tgatgacggt gatagaaatt gttgaacttc tgcgccaact gaaatgcgtg
     3301 cttggcagcg tacgccggct cggttgtggc tatgcactgc tcgatcaccg atgacgtctt
     3361 cccggccaat agccagagct cccatatctc ggtgccgctg tcttcggcga agaagcgggc
     3421 gtagtcgaca tcagccgaga gagcctgctc gggttccact tcagctttgc ggaatatgtt
     3481 gctggcacgc accacggcgt attgcgtgta agggccggtt tcgccctcga aagccagcgc
     3541 ttccttaaag tcgaaggcga tcaccgattg cttcgtgaac ttcagcatga agtagcgcag
     3601 cgcgccgatg gctatctgtt tggagatttg taagcgctcg gtatcgtcga gttcgggatg
     3661 gcggctgtcg acttccgtct tggccgcggc aattagggcg tcgagcagat cgtcggcctt
     3721 gactccaaat cccttgcggc ccgacacctc gatatacgac ttcgcctggt cttccgcgct
     3781 cacctcatac ccgaggtcca tcgcgcagcg gggagtgagt gcgaccattt cgtaggagaa
     3841 atgcgtgtag tgcgcagcct tctcgggata gcccagcaat cgtatggcct cgaccaccgt
     3901 attctgagga tcgctctggc gcgagtcgat tacgttgtag attgctgcga cgcttccgaa
     3961 gtgagggtgg tcagcttcgc cgtcttcggc cgagatccag cactcgtggc cttttggata
     4021 gcgaaaaaac ttctgatatc caaagtcgcg tcccagcaat ccaaacttcc acagatggta
     4081 ggcgatgtct ttgccaacat agcccactgt tccgttcgaa cgcacgatga ctttagcgtc
     4141 ttcgtcaggt cccgttttcg cttcggcgtc agacgactct gcatcgctgc cgcgactggc
     4201 gttgaccttt gtctgggtag ccgggcgggt catcacccag cagcccttgt tcttgccttc
     4261 agtctcgaag tacagcacgc ccttttcttt gagctgttgg aaggccaggt cccagaagtg
     4321 caggcgcaaa atctcgctct cgcgggggag aaagtcgtac tcgatgtcga gacgatccat
     4381 cgtctccaaa tggcgtcgta aaacagccgt cgagaccatc tccgcaatct cagccatctc
     4441 attgtttccc tgctcgatgg catgtagtat ttcaagtctt gctttaagat tttccttgtc
     4501 ctgaccgtac cagtgcgaca cgcttgcgta caggtcccag cagtaatagt cgaagcgcgg
     4561 tgtggaggcg atcagcgcct cgatctcggc cttcgacagc ttcgccaggc gaacgaaacc
     4621 gactaccacg tcagccacct ggacgccggt gttgtcgatg tagttctgaa tgtctaccgt
     4681 gtggccggca gcacgtagta gtcggacaaa cgtatccccc aggattgcgt tgcgcagatg
     4741 cccgatatgt gcggccttgt tgggattgat gctggtgtgc tcgacgagaa tcttgcctga
     4801 cacgttcgcc gcgccttcgg cagcgtggtc ggttacagcg atggcggttt ccctcgcaag
     4861 cgctccggca gcttggtcac ggacgaggcg ggcattgatg tatcccgcgc ctgcaacttc
     4921 gaacttggcg aatccgtcgg gcagcggcat ctcaggaacg atttcgtcgg ctatcttacg
     4981 cggagccttc cgcaactgct tggccaactc aaacggaaac ggcatggcga actcgcccat
     5041 ttcgatcttc ggcggctggt cgatggcgat gttcggcagg tcgatctggt acttctggcg
     5101 gaggaattct cggacgtggt tggccagttg tttctgtcgc tctagataca cgattgctct
     5161 cggtctctct cgctgcgctg ggactattcg ctgcgggcat tgcgagacgg aatgtcgact
     5221 cgcaaatagt tgaggaaaaa cgtgattata acaaacgcct gcaatgacgc gggtttgacg
     5281 ctgatcgcac cgttacctat gatgagagtt cccaccaggc gtcttccatc gtgctggtgc
     5341 ggcataccgg cgggtcgatt cccatgcgaa ttgcatatct ggacggattt tccggcatta
     5401 gcggcgacat gttcctcggc gcgctggtgg atgccggagt tccgcttgaa ttgttccagg
     5461 agaccgtcgc ggcgctgcat ttagggatca ccttggaggt ctcgcgcgta gatcgcaacg
     5521 gcatttccgc caccaaggtc gatgtaatcg tgaacggcca gaaggatctg ccgcgtgagg
     5581 aattctgggc tcaaaacgaa cacctgagct cggggcgtgc ccgcgaatcc gcagccgatc
     5641 atgcacacga ccacgggcac actcacgcgg acgaacatgt gcacgcccac ggtcgaaatc
     5701 tcaacacgat tctcgggatt attgattccg cgcccctcag ccgccgcgcc aaactgacag
     5761 cgagcgaaat cttcgtcgcc ctgggagctg ccgaagcaaa agtccacaat gtcgacatag
     5821 ccgacatcca cttccatgaa gttggcgctg ccgacgcaat cgccgatatc g
//
LOCUS       DASRKK010000274         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_117782, whole genome shotgun sequence.
ACCESSION   DASRKK010000274 DASRKK010000000
VERSION     DASRKK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117782"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..307)
                     /locus_tag="VFC15_06030"
     CDS             complement(92..307)
                     /locus_tag="VFC15_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09754.1"
                     /translation="MAILTRKLGLDVRSGNWSVFSPKPFTYARLATSPRRDAVDQKQV
                     ERLKQLAYEAAVAELVQAHSELAMTGH"
     gene            470..2194
                     /locus_tag="VFC15_06035"
     CDS             470..2194
                     /locus_tag="VFC15_06035"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM09755.1"
                     /translation="MQRLEAKSIHGRTYYYLSLWGWKNGKCRRLSQKYLGKPEDIARA
                     VEGTGSAPQYAVVLDWGLPQALWQEADRAQLVQQVDQLCPKRAQGLSTGDYIRLAAIN
                     RACEPVSKQAMWEWVSHTALPRLWPEASAPQLTSQHFWDHMDRIPPETAQAIWQQLIA
                     AVLQREQIQLSQVCYDGTNFYTFIDTFNVRCQVAKRGKNKQGRANLRQVSYALFCTTD
                     GHLPLFYDVYEGNRNDAKEFPQVLEKFQRWLRQRAGSTWATTQPTLIFDKGNNSADNF
                     ALIDALELPYVGSVKLDEHPDLADVSNRDARWQALTAPGLEGTKSWRTQQVVYGRERT
                     LVLTFNPNLYDSQWATVQNDLAQALSQLAVVQQSLQNRAAGLVKGGTAPTLESVRRKC
                     EQILHRQHLRQLVQTTVSLNSAAMPQLSYQARAEAQQQLKDTYLGKTLLITGHNEWTD
                     AQIIRAYRSQFVIEEIFHEMKDRHIGAWWPLHHWTDSKIQVHGLYCTMAVLLRALLWR
                     RAHQAGLHLSMSALLHALSQMRQVVNVFPSKRTGQPKPEQTVLTQRDDTQEKLIEILG
                     LRTVKSAI"
     gene            complement(2229..>2469)
                     /locus_tag="VFC15_06040"
     CDS             complement(2229..>2469)
                     /locus_tag="VFC15_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09756.1"
                     /translation="QAGSEAKPHVFLAVGTFQDLSLNNRLAEFPRQLRTQLIEAYQGS
                     EITLLEREYVNELLQEVRLDLAPVYELIQACFGAS"
BASE COUNT          551 a          750 c          687 g          481 t
ORIGIN      
        1 tgctgatagg gttcctggat aggtacgagt gccacaaatg ggccgtccgc atcttcgagc
       61 gcgaatcgtc cctttgctta aacccgaatt ctcaatgccc cgtcatggcg agttcggagt
      121 gggcttggac caactccgcg acggccgctt cgtaagccaa ttgcttcagg cgctcgactt
      181 gtttctggtc aacggcgtcc cgcctggggg aggtggcgag gcgggcatag gtaaacggct
      241 tggggctgaa gactgaccaa ttgccggagc ggacatctag ccccaattta cgagttagga
      301 tagccatttt cgtgtaagtc acttcgatac agtcgcttgc aacgcaagta gcctatgccc
      361 taagcacttg cctggctaac ggcaatgttt tcttgatata ttgcggcttt tctgttgaca
      421 gtagatccat actatgccct aaatactgcg tcacgacaac tgacgccgta tgcaacgact
      481 cgaagccaaa tccatccacg gccgcaccta ttattatctc tccctctggg gctggaaaaa
      541 cggcaagtgc cggcgcctgt cccaaaaata cctcggcaaa ccggaggata ttgcccgagc
      601 cgttgaaggc accgggtcgg ctccacaata cgccgtggtc ttggattggg gcctgccaca
      661 agctctctgg caggaggccg accgagccca actggtccag caggttgacc agctatgccc
      721 taagcgcgcc cagggcttga gcaccggcga ctacatccgc ctggcggcga tcaaccgcgc
      781 ttgcgagccg gtaagcaaac aagcgatgtg ggagtgggtc tcccataccg ctttgccccg
      841 cctttggccg gaggcctcgg caccccaact gacctctcag catttctggg atcacatgga
      901 ccgcatcccg cccgaaaccg cccaagccat ttggcagcaa ctcatcgccg ccgtcttgca
      961 gcgcgagcag atccaactgt cccaagtctg ctacgacggc accaacttct acaccttcat
     1021 cgacacgttc aacgtcaggt gccaggtggc caaacgcggc aagaacaagc aaggccgggc
     1081 caatctgcgc caagtcagct atgcgctgtt ctgcacaacc gatggacatc tgccgctctt
     1141 ttacgacgtc tatgagggca accgcaatga tgccaaggag tttccccagg tcctggagaa
     1201 gtttcaacgc tggctccgac aacgggcggg ttccacctgg gccacgaccc aaccgacgct
     1261 cattttcgac aagggcaata actccgccga caactttgcc ctgattgacg ctctggagct
     1321 gccgtatgtg ggctccgtca aactcgatga gcatcccgac ctggcagacg tttccaaccg
     1381 ggacgcccgc tggcaggcac tcaccgcacc gggcttggag ggaactaaaa gctggcgcac
     1441 ccagcaagtg gtctatggcc gggagcgtac cttggtgctg accttcaacc caaacttgta
     1501 tgacagtcaa tgggcgaccg tgcagaacga cttggcccag gcgctgagcc aactggcggt
     1561 cgtgcagcaa agtctccaaa accgggcagc cggcctggtt aaggggggaa ccgcgccgac
     1621 gctcgaatcg gtgcggcgca agtgcgagca gattcttcac cggcagcatc tgcgccaact
     1681 ggttcagacc accgtgagcc tcaactcggc ggcgatgccc caattgagct atcaagctag
     1741 ggcggaggcg cagcagcaat tgaaggatac ttacctgggc aaaacgctgc tcattacggg
     1801 ccacaacgaa tggaccgatg cccagatcat ccgcgcctac cggagccagt tcgtgatcga
     1861 agaaatcttt cacgagatga aagaccgtca cattggcgct tggtggccgc tgcaccactg
     1921 gacggacagc aaaatccagg tccacggact gtattgcacg atggccgtgc ttttgcgcgc
     1981 gctgctatgg cggcgagccc accaagcggg cctgcactta tccatgagcg ctttgttgca
     2041 cgcgttgagc cagatgcgtc aggtcgttaa cgtcttcccg agcaagcgca cgggtcagcc
     2101 caagccggaa cagaccgtgc tgacccaacg agatgacacc caagaaaaac tgatcgagat
     2161 actcggattg cggaccgtca aatcggcaat ttaggggata ggggaccgga gtcggatgtt
     2221 gctgattatc agctagctcc gaagcacgct tgaattaact cgtaaactgg ggctaggtcc
     2281 aaacgcacct cctgcagcaa ctcgttgacg tattcccgct ccagcaaggt gatttccgaa
     2341 ccctggtaag cctcaatcaa ctgggtgcgc agttgccgcg gaaattcagc cagccggttg
     2401 ttaagactca ggtcctgaaa cgtgcctacc gccaggaaca catgcggctt ggcttcgctg
     2461 ccggcttga
//
LOCUS       DASRKK010000275         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_20678, whole genome shotgun sequence.
ACCESSION   DASRKK010000275 DASRKK010000000
VERSION     DASRKK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20678"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFC15_06045"
     CDS             <1..877
                     /locus_tag="VFC15_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZM09757.1"
                     /translation="PIKEWKYPYTIGQTVYAWLPWTILILCCAVWGMPTWKAHLNTLL
                     ASVKINTTLLGSKLAGTLSLPTWQMPALHNLVQRMPPVALPNAKPEGAVFNINWFSAA
                     GTGVFVAALLTGLTLKLSAAQWMEATTRTLRRMKIPILVIGQVLGLGFLTRYSGTDAV
                     LGLAFTSAGPFYPFFAAYLGWLGVFLTGSDTASNALFGSLQRITAQQLHLNEVLIVAT
                     NSTGGVMGKMIDAQSIMVACAACYDDPYERSHALGPIFRKLFWHSIVLAGIIGLIVML
                     QAYVFPGMIPHLPGK"
     gene            970..1272
                     /locus_tag="VFC15_06050"
     CDS             970..1272
                     /locus_tag="VFC15_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HZM09758.1"
                     /translation="MKRKASVSHDEAMVRRLRKDPDFAAEYLKAALEDEDEPRVLLLT
                     LRHLAQARGIAKVDKAAGIERESLYRALSARGNPRLSTLVAVTKAIGLRLTVEAAP"
     gene            complement(1303..2859)
                     /locus_tag="VFC15_06055"
     CDS             complement(1303..2859)
                     /locus_tag="VFC15_06055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZM09759.1"
                     /translation="MTTSLTTFSLSYSSTDYLLALPMLLLTVFALGILMIDLLLPKQW
                     KRVNAITALIGVAFSAAAVGKLHLAYHAAEKQGIEIATFTGFMGSLVVDRFAIYFFYL
                     FLMGAGIAILMSMRYLEIERENHGEFYALMLLSVVGMMCMAAGYDIVLIFIGLELMAI
                     STYVLVGFLRRDKRSNEAALKYLLLGAFSSGIFAYGLSLFYGLTGSTNLGQIAQKLQE
                     VIATRPNDPIVIFALLTTTTGLLFKIAAVPFHQWAPDAYEGAPTSITGFMSVAVKSAG
                     WAMLLRIFLFGLYPLRPMYVPLLIFVAVATMTGGNLAAITQDNLKRLLAYSSIAHVGY
                     MLLGLIASDGQTNSTGIMAIMIYLLVYTFMNLGAFAVITSLRRRNIIGDTVDDIAGLY
                     FKAPTEAMLMLIFLLSLAGIPPLAGFWGKYFIFLSLIETGHYTLAAIAVLYAVISMYY
                     YMRIANAMLMRQPIDAEPVHTRPAMRLALTITAAGTVGIGLFPNFFINVANWSLGLVQ
                     GTQHMARMLR"
     gene            complement(2870..>3691)
                     /locus_tag="VFC15_06060"
     CDS             complement(2870..>3691)
                     /locus_tag="VFC15_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZM09760.1"
                     /translation="INHGISTGALFLIVGILYERRHTREISEYGGISNVMPVYATITL
                     IMFMSSMGLPLLNGFVGEFTILQGTFMENKVWAAWAVPGVILAAAYLLWLYQRVFFGP
                     VNNPKNEKLLDLSGRELATFIPLVIVAFWIGLYPKPFFQILSTPVNNLVATVRPDYPG
                     LTKPVLAAQPVNPNPDAATPLPEPPKSPATQTSPAGGTPSQTGVTQTHPPVRVVRRPS
                     TSPGQAFSAASGTNSSVGSRALSPAAPSTKNQAQKPVPVNTAKANLAAAPAALGK"
BASE COUNT          777 a         1117 c         1073 g          724 t
ORIGIN      
        1 gccgattaaa gagtggaaat acccatacac catcggccag acggtatatg cctggttgcc
       61 gtggaccatc ttgatcctct gctgtgccgt ctggggcatg ccaacctgga aggcccacct
      121 caataccctt cttgccagcg tgaagatcaa cacaacgctg ctgggctcga agttagccgg
      181 taccctctcg ctgcccacgt ggcagatgcc cgcattgcat aacctggtgc agcgcatgcc
      241 gcctgtggcg ctgccgaacg ccaagccaga gggtgctgtt tttaacatca actggttttc
      301 cgctgccggt accggcgtct ttgttgccgc cctgctcacc ggcttgaccc tgaagttgag
      361 cgccgcccaa tggatggaag cgaccacccg caccctgcgc cgcatgaaga tccccatcct
      421 ggttatcggg caagtactgg gcctgggctt tttgacccgc tactccggca ccgatgcggt
      481 cctgggactg gccttcacca gcgcgggtcc cttctatcct ttcttcgccg cttatcttgg
      541 gtggctgggt gtcttcctca ccggctccga taccgcttcc aatgcgcttt tcggaagcct
      601 gcaacgcatc acggcgcagc agcttcactt gaatgaggtc ctcatcgtgg ccaccaactc
      661 cacgggcggc gtcatgggta agatgattga cgcccagtcc atcatggtcg cctgtgctgc
      721 ctgttacgat gatccttacg agcgttccca cgctctgggg ccgatcttcc gcaagctgtt
      781 ctggcactct atagtactgg ccggcatcat cggcttgatc gtcatgctcc aggcatacgt
      841 cttcccgggg atgatcccgc acctacctgg caagtaaaag cggccatggg tagtggctca
      901 attgagccac tacccgttga ccgagcatta cgctttctca gggagtacaa agacaaggac
      961 cggaatccaa tgaaacgtaa agccagcgtc tcccacgacg aggccatggt gcgccgcctg
     1021 cggaaagacc cggactttgc cgcggagtac ctcaaggctg cattggaaga tgaagacgaa
     1081 ccacgagttt tgcttctgac cttgcgccat ctcgcccagg cgcggggtat tgccaaagtc
     1141 gataaagctg cgggaattga gcgcgagagt ctttaccggg cattatcggc tcgcggcaac
     1201 ccccgcctat cgacgttagt cgccgtcacc aaggccatcg gtttgagact caccgtggaa
     1261 gccgctccat aggcgcactc tcccggccac tgtttccagt tttcaccgca acattctcgc
     1321 catgtgttgc gtaccctgca ccaatcccag cgaccagttc gcaacattga taaagaagtt
     1381 cgggaacaat ccaatcccca ccgttccagc agcggtgatg gtcaaggcca gccgcatcgc
     1441 cgggcgggtg tgcaccggct ccgcatcgat cggctgccgc atcaacatgg cgttggcgat
     1501 gcgcatgtag tagtacatgc tgatcacggc atacagaacg gcaatggccg ccagcgtgta
     1561 gtggcccgtc tcaatcagac tgaggaagat aaaatacttg ccccagaagc ccgccagtgg
     1621 cggaatccca gccagcgaga gcaggaagat cagcatcagc atggcttcgg tcggcgcctt
     1681 gaagtacaac cctgcgatgt catcgaccgt gtctccgatg atattgcgcc gccgcagcga
     1741 tgtgatcact gcgaacgcgc ccaggttcat gaaggtgtac accagcaggt aaatcatgat
     1801 ggccatgatg ccggtggaat tggtctgccc gtcgctggcg atcaggccca gcagcatgta
     1861 gccgacgtgg gcgatggagg agtacgccag cagccgcttc aggttgtcct gcgtgatggc
     1921 ggccagattg ccgccggtca tggtcgcaac cgcaacgaag atcagcagcg ggacatacat
     1981 cgggcgcagc ggataaagtc cgaataggaa aatgcgcagc agcatcgccc atccggcgga
     2041 tttcacggcc accgacataa atccggtaat gctggtgggc gcgccttcgt atgcatcggg
     2101 agcccactga tggaagggta ccgccgcaat cttgaacaac agtccggtgg tggtggtcag
     2161 tagcgcaaag atgactatgg gatcgttcgg ccgggtggcg atcacttcct gtagcttctg
     2221 cgcaatttgt cccaggttcg tgctgccggt caaaccgtag aacagcgaca gcccataagc
     2281 gaagattccc gatgagaacg cgccgagcag caaatatttc agtgcggctt cattcgaccg
     2341 cttatcgcgc cgcaggaatc cgaccagcac gtaggtcgag atggccatca gctccaggcc
     2401 aatgaaaatc agcacgatgt catagccggc agccatgcac atcatcccga ccaccgagag
     2461 cagcattagc gcgtagaact cgccgtggtt ttcgcgctcg atctcgagat aacgcatcga
     2521 catcaggatg gcaatgcccg cgcccatcag gaacagatag aagaaataga tggcgaagcg
     2581 atcgaccacc aacgagccca tgaatccggt gaaggtggcg atttcgatgc cttgcttttc
     2641 cgcggcgtga tacgcgaggt gcagcttgcc caccgcagcc gccgaaaacg cgaccccgat
     2701 cagcgcggta atggcgttga cccgcttcca ctgctttggc agcagcaagt cgatcatcag
     2761 gatgccgaga gcgaacaccg tcagcagcag catcggcaag gcgagcaggt agtcggtact
     2821 tgagtaagac aaagaaaacg ttgtcagcga ggtggtcact atttcatcct cacttgccga
     2881 gtgcggccgg ggccgccgct aaattggctt tcgccgtatt cacgggaact ggcttctgcg
     2941 cctgattctt cgtcgagggt gccgccgggc tcaaagcccg gctccccact gaagaattcg
     3001 tccccgacgc ggcgctaaaa gcctgccctg ggcttgtcga agggcgccgc actacccgaa
     3061 ccggtggatg cgtttgcgtc acgcccgttt gcgatggcgt gccgcctgcc ggcgacgtct
     3121 gggttgccgg cgacttgggt ggttcgggca gcggagtcgc tgcgtcggga ttcggattca
     3181 ccggctgagc ggcaagcact ggcttagtca gtccggggta gtcggggcgc accgttgcca
     3241 ccagattgtt gaccggggtg gagagaatct ggaagaacgg cttgggatac agcccgatcc
     3301 agaaggcgac gatcaccagt ggaatgaagg tcgccagttc ccttcccgag agatccagca
     3361 gcttctcgtt cttcggattg ttcaccggcc cgaagaacac gcgctggtag agccacagca
     3421 agtaagccgc ggccagaatc actcccggca ccgcccaggc cgcccacacc ttgttctcca
     3481 tgaacgtgcc ctgcaggatg gtgaactcgc cgacgaaccc gttgagcagc ggcagtccca
     3541 tcgaagacat aaacatgatg agcgtgatgg tggcgtaaac cggcatcacg ttggagatgc
     3601 cgccgtactc cgagatctcg cgcgtgtgcc gccgctcgta caggatgccc acaatcaaga
     3661 acagtgcgcc ggtggagatg ccgtggttga t
//
LOCUS       DASRKK010000276         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1389108, whole genome shotgun sequence.
ACCESSION   DASRKK010000276 DASRKK010000000
VERSION     DASRKK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389108"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(879..2000)
                     /locus_tag="VFC15_06065"
     CDS             complement(879..2000)
                     /locus_tag="VFC15_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09761.1"
                     /translation="MTISGKQRGKLKIFMGYAPGVGKTYKMLEESQELKSQGVDIVVG
                     YFESHGRPDTIAKTEGLEIVPRKRIDYRGSQFEEMDTDAILARHPSACVVDEFPHTNV
                     PGLDRAKRWEDVEVMLDAGIDVLTTMNVQHLESLNDHVWNITGIRVRETIPDWVVRQA
                     DEVVMVDLTPRALLHRLERGVVYGREKAQRAMENFFRESSLVALRELTLREAAHEVKD
                     RIGDDATGAPAEERIQSDKRKHQKVLVMVTSDPKTVMVIRRARRVSDFLGAECFAVAV
                     QPSEDLSGVPEPEREAIEKHLSFARNLHIETRILGGEDAAAVLLDFARRNGVTQIFVT
                     RPSGRMWVPLLSRDPVQRIISQAKDMQVVIVSDRAPVPR"
     gene            complement(1997..>2504)
                     /locus_tag="VFC15_06070"
     CDS             complement(1997..>2504)
                     /locus_tag="VFC15_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZM09762.1"
                     /translation="SDAERHRQQLSKLRELALVLAADVVDHQLSGYLDRSGISQQFGA
                     HERILVCVTPRANVQEMFDLARIFAERFHGELIVAYVSQPQISPADQATLDQKLAIAK
                     AAGARIEILDGDDPVDALLEFARSRGITQLFIGHTQRTGIWVRLWGSPFDRLIRLSRG
                     MDVRVFPQ"
BASE COUNT          496 a          748 c          725 g          535 t
ORIGIN      
        1 cgttgccact ggttccgggc caggcgagcg ccggtgcccg tgcccgcgcc ggtgctccgc
       61 cgccaagtga ttcgggatta gccatgaacc agaggtagga gacaaatgac gcgttgcagt
      121 tgtccagggc ggtccaagac gccaaaggat atggcatctc gtgatgtcag ggaccagtga
      181 catcacatcg cggccaaaag cagcaatgaa ggggttgctg ttaaccatcc ggtgggctgc
      241 taaccaacgg gtatcaagaa gtcagtgcct gcaaggcaaa tggctggcga caaggctacg
      301 cacatctcgg gctaaacttc cgcgccgcag ctctgcgatg agcaggcgtc atcttgtcaa
      361 caccttgagg agttgggtcc attgcgccca gccggtcgct ggtaaccacc tggtggtctc
      421 cacggtttgg cttggctccc caataagacg gctttccaac tgtttctcca gagcgttgtt
      481 gggatgtggg tagatttctt cgtgcttgcg ccgaaccaga acagcgggtg ggcgctggtg
      541 aactcttcga ttggtttgag cgtatctgcc ggcaacgcat gtgcgcattt cttcgcgaat
      601 cgaaggacgc gctcgatccc aaggcgtcca agcagcgtgc gcgtaacggg acaaacgagc
      661 caaatgcgag tatccgaaac agggaggaag cgtgcgaggg tgaaagccgc cgttgcttcc
      721 gccgctgtcg tgaacaacac gatgatccgc gttgcgcacg ccgcggaatc tcccaaggcc
      781 caaggctcac cacgctcggc atgcagcaaa gcagcacttc ttcaattgct tgtttggaaa
      841 cgactcttac gtacatcgtt aaggtcgctg ttgtgttctt agcgcggcac cggggcccgg
      901 tcggagacga tgacaacctg catgtccttg gcctggctga tgattctctg taccggatct
      961 ctggacagta gcgggaccca catgcgcccg ctgggccggg tgacaaagat ctgagtgacc
     1021 ccatttcggc gtgcgaaatc aagcagcact gccgcggcgt cctcgccccc gagtatccga
     1081 gtctcgatat gcaagttgcg cgcaaaactc aggtgcttct cgatcgcctc gcgctcgggc
     1141 tctggcacac cactcaagtc ttcagatggc tgcacggcga cggcgaagca ctctgcaccg
     1201 agaaaatcac tcacacgccg ggcacgccgg atcaccatca cggttttcgg atcagaggtt
     1261 accataacga ggaccttctg gtgcttgcgt ttgtcgctct ggattcgttc ttcggctgga
     1321 gcgccggtgg cgtcgtcccc gatccggtct ttcacttcat gagctgcttc gcgcagggtg
     1381 agttcacgca gcgcaaccaa ggacgattcg cgaaagaagt tctccatggc cctttgtgcc
     1441 ttctcccgcc cgtacacaac cccgcgttcc agccgatgca gcagagcccg aggcgtcaga
     1501 tcgaccatta ctacctcgtc ggcctgccga actacccagt ctggaatcgt ttcgcggacc
     1561 cggatgccag tgatgttcca tacatggtcg ttcaggctct ccaggtgctg tacgttcatc
     1621 gtcgtgagaa cgtcgatgcc ggcgtccagc atcacctcca catcttccca ccgcttggct
     1681 cggtctaaac ctgggacatt ggtgtgcgga aattcatcga cgacacacgc gctcggatgt
     1741 ctcgccagaa tagcatcggt gtccatctct tcgaattggg acccgcgata gtcgattctc
     1801 ttcctgggca cgatctcgag cccttcggtc ttagcaatcg tgtctgggcg gccgtgggac
     1861 tcaaagtaac cgacaacaat gtcgacaccc tgcgatttca gctcctggga ctcctcaagc
     1921 atcttgtagg tcttgccgac accgggggcg tagcccatga agatcttgag tttcccgcgc
     1981 tgttttcccg aaatggtcat tggggaaata ctcgaacgtc catgccgcgt gagagccgga
     2041 tcagtctgtc gaacggactg ccccacagac gcacccagat tccggtgcgc tgggtatgtc
     2101 cgatgaagag ttgggtgata ccccgggatc tggcgaactc aaggagtgcg tccactggat
     2161 catcgccgtc cagaatctcg atccgggcgc cagcggcctt ggcgatggcc agcttttggt
     2221 cgagggtagc ttgatcggcg ggtgagattt gcggttggct gacatacgcc acaatgagtt
     2281 ccccgtggaa cctctccgcg aagattcgag ccaaatcaaa catctcctgt acattggcac
     2341 gcggcgttac gcatacaaga atccgctcgt gcgcgccgaa ctgttggctt ataccgctgc
     2401 ggtccaggta gccgctgagc tgatgatcca cgacgtccgc ggcgagtaca agcgccagtt
     2461 cccgcagctt cgatagctgt tgccggtgcc tctccgcgtc agac
//
LOCUS       DASRKK010000277        15014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_411663, whole genome shotgun sequence.
ACCESSION   DASRKK010000277 DASRKK010000000
VERSION     DASRKK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411663"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1301
                     /gene="trxB"
                     /locus_tag="VFC15_06075"
     CDS             342..1301
                     /gene="trxB"
                     /locus_tag="VFC15_06075"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZM09763.1"
                     /translation="MTDNLRNVIIIGSGCSGLTAAIYAARANLRPLVIDGHEPGGQLA
                     ITTLVENFPGFPEGIQGPELIDNMRKQAARFGAEFTTGHVVSVDLSQKTEGLGTKPIE
                     VSLGRETLLTRALIVASGASARWLDLPNEKALIGHGVSSCATCDGFFFSGKEIAVIGG
                     GDSAMEEALFLTRFASKVTLIHRRSEFRASKIMLDRARQHDKIEFLTDTVVDDVYDVS
                     KKEVTGLRLRNLKTGEQWDFPTSAMFLGIGHTPNADFVGDQLARDGEGYLVLSDGHVC
                     TKVPGVFISGDVADRHYRQAVTAAGTGCMAALQVEKYLEEIGK"
     gene            1348..2229
                     /locus_tag="VFC15_06080"
     CDS             1348..2229
                     /locus_tag="VFC15_06080"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZM09764.1"
                     /translation="MPKPPSTESDADEFRRAELTAQFILGRTALRPRVALVLGSGLGG
                     FADELENAVRIPYAEIPSFPRSTALGHAGTLVIGTVSGIAVVAMQGRVHLYEGYSVQQ
                     VAFPVRVFGRMGVRAVVLTNAAGGINPSYGRGALVVIRDHINLQGGNPLVGANEDRFG
                     PRFPDMTDAYNAEFRKFALDEKGRMGDDIYEGIYAAMLGPSYETPAEIRFLRTIGADL
                     VGMSTVPEVVVARHMGLEVLAISCVTNMAAGMSGEKINHEEVLETGERVRGKFLALLR
                     TVLPRIGQYLDARSASF"
     gene            2280..2957
                     /gene="udk"
                     /locus_tag="VFC15_06085"
     CDS             2280..2957
                     /gene="udk"
                     /locus_tag="VFC15_06085"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HZM09765.1"
                     /translation="MSGKSYLIGIAGPSGAGKSYLAHHLSQRLQAPVLPLDHYYRDLS
                     HLPLEERARSNFDDPAALEHELLIEQVAQLCEGIAIEIPTYDFSVHTRTGETQSFQPE
                     PFVIVEGLFTLHWPELRRLLGTRVFVNMTDDICLQRRKDRDVRERGRTPESVVEQFRT
                     TVAPMAERYVRPTQAHAHVVVFGSIPIEDEVERVLEHVRQNTQEPGLPPAARHIRRSP
                     SPSVPSR"
     gene            2966..5776
                     /locus_tag="VFC15_06090"
     CDS             2966..5776
                     /locus_tag="VFC15_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09766.1"
                     /translation="MHRRRIIVVLVVVWLCITSLTAQVVAQKAQKEVPLSTSKTLLEP
                     VPGSPQRTNSLPVTAALSPDGKYLALLNDGFGSAESDYRQSISVLDLSRDQLRDFPDS
                     RLGTLAQQTYFLGLAWSGDGHELFASMASLTDPEGKKPGNTGNGIAVYRFTYGALTSD
                     RFLKLPLAPLGKGKKNVYGSKYVPAGFVSPYPAGIGVIRHDGRGALLVAENLADDAVL
                     IDERDGKVLQRFDLGRGKVVPSTFPYTVIVNREGTRGWCSLWNGSSVAELDLRSGKVM
                     RQIVLLPPKEETDASSHPTALLLSPDERRLYVALANRDRVAIIATTDGKIERYFDTRL
                     PGQTYGGNYPIALAQSIDGMQLYVADSSSDAVAVFDLRETRGGPATANQDRASYFIPT
                     EWYPTALALQDNDLFVATGKGMGTGPNSGTQAVPLLDHKSPHPYIASLIRGSIARVNL
                     KDAERDREALTAEVMQSNRMEGRTNQITFQRGGNPIHHVIYIIKENRTYDQLFGDIKE
                     ANGDASLVMYGDDITPNQHKLARQFGVLDNFYDSGEVSGDGHPWSMAAITTDYNEKAW
                     PVAYRGHEREYDSEGTIGDVVPLNENIPDVNEPATGYLFDNLARHRMSYRHYGEYIET
                     RWCEDLEQNYTPAATGAPAAHAPECERRDIKPGEELPAQLGGGKNPYQHAIPLMAYNI
                     PVKADLRNHFDPDYPDFKVEFPDQFRVDIFLREFTGFVAARKSGKGEQLPSFALVRLP
                     NDHTAGTKVGSPTPNASVADNDLAVGRVVEAISNSPYWDDTAIFVLEDDAQNGADHVD
                     AHRSIGLVISKYSPQSPQPFVDHNFYTTVNLMHTMETLLGLPPMNNNDAHAAVIAPLF
                     TGAGDQPAFKAEYRNRDNGMIYQANTKDAPGAKESAALDFSVADAADTGILNAILWRA
                     AKGDAAMPEPRHTVFPVSPGEK"
     gene            6158..8725
                     /locus_tag="VFC15_06095"
     CDS             6158..8725
                     /locus_tag="VFC15_06095"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZM09767.1"
                     /translation="MRTAEMLREAILRHVRYTLVRPTSDLKAADYLKPVSLAIRDRIV
                     DRMLETESRFRHKDAKRLYYLSMEFLMGRLLGDNLSNLRIEELCREVLAGFGIDLDDV
                     LESESDAGLGNGGLGRLAACFLESLATLGMPGYGYGIDYEFGLFKQEIFNGFQREKPD
                     RWKANGTPFQIEHPEEAISIPMYGRMDSQRDADGNLKQTWINYKIVLGIPTDIPIVGY
                     LGQTVNWLRLFAARASEDFDIDIFNRGDYLRAVEQKIGSENISRVLYPSDTAMAGKEL
                     RLVQEYFLVACAVGDIMRRFRQQHDDIELLPAKAAIQMNDTHPSLAVAELMRVLLDQY
                     GLPFDKAWDITRRTLAYTNHTLLPEALEKWSVPLMEKVLPRHIQIIFSINHRFLREIQ
                     LEAVIDGEKLRKMSIIEEGYEKHVRMAHLCIVGSHSVNGVSELHSNLLVKSLVPEFAE
                     IWPDKFNNKTNGVAPRRWLMKANPGLTELISQTLDEQKWITDLSRLRDLEPFAEDPEF
                     RAAFKEVKRQNKLKLAGAIQAQVGVVVDPDSIFDVQIKRIHEYKRQLLAVMNVIHDYL
                     RIVEHAETLASARTFVFAGKAAPGYWAAKQIIKLIHNVADVVNNDERVKDAIKVTFLP
                     DYRVSLATLIIPGADLSEQISTAGMEASGTGNMKLSMNGALTIGTWDGANIEIAEAVG
                     LNNIFIFGLRAEEILEMQQKGSYNPRERYDNDPDVKEVMDALASDRFCPNEHGLFRWI
                     FDELVHRGDRYYHIADFPSYVETQKQIAQEYRQDEIWYRKSILNVARIGKFSSDRTIL
                     EYARDIWHIGPYEKSTPVTKAAGKSHTPVAPLPSAVNGDAIPVVELTQEVEPNPITE"
     gene            complement(8768..8929)
                     /locus_tag="VFC15_06100"
     CDS             complement(8768..8929)
                     /locus_tag="VFC15_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09768.1"
                     /translation="MALQLRRHGIRNVRPLLGGYHAWKDLGYPVEEVPEGSGAGGSDE
                     AVATEDLTT"
     gene            complement(8954..9760)
                     /locus_tag="VFC15_06105"
     CDS             complement(8954..9760)
                     /locus_tag="VFC15_06105"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZM09769.1"
                     /translation="MAETLQFVVQHGYLLLFFWVLMEQAGLPIPAMPLLLAAGALAGQ
                     HQLNFALVVLVAATGSLFSDTFWYFFGKRKGPIVLNFLCKIALEPDSCVRRTESTFTR
                     FGLQTLLICKFVPGLNTAAPALSATAGIGLPQFLLYDFMGALLWSSAFAGLGLLFSRQ
                     LDRVAHDVAQFGSWTLLLVIAGIGGYVAYKFYERHRFLKQVAGFRITPEELKEKLEAG
                     EPMTIIDLRHPLDLLPDPQTLPGALRISPEELESRQSEITRGGEIVLFCT"
     gene            complement(10142..10978)
                     /locus_tag="VFC15_06110"
     CDS             complement(10142..10978)
                     /locus_tag="VFC15_06110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZM09770.1"
                     /translation="MCVGLLLTGIACSKKPAGKRYELQGRVVAVDSGSREITVAHQDI
                     PGLMPGMTMPFPIARDQDWVFGKIGPGDHIHATLVMTDHAELQDISFTQGSDTGSDGT
                     SNLRIPEIGDDVPDFTLVNQFGKTIHLDQFRGKPLLLTFIYTRCPVPDFCLLMSNNFS
                     AVLKELQKNPAVFAKAQLLSISIDPNYDTPIVLQSYGKRYVGDVDPGFHHWQFASGSP
                     EEVRKAADFFGLSYNEKQGQIVHTLQTVVIGADGKIAKVYTGNQWKPDEVAAEYEAAA
                     SH"
     gene            complement(11355..12701)
                     /locus_tag="VFC15_06115"
     CDS             complement(11355..12701)
                     /locus_tag="VFC15_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM09771.1"
                     /translation="MRPGCGGWREGASHRKLFHDPLRVNLVAMHPEVIVVGSGVSGLV
                     AAVELQRRGLSVQVVEASNDVGGRIRTDEVDGFLLDRGFQVLLTAYPEARRCLNFGAL
                     ELREFEPGAIVRHNGRFHTISDPIRRPRKILPSAFAPLGTFNDKLLIAKLRGEVLDTE
                     IDLVLSAPEVSTIAALRKYGFSGKIIEHFFRPFLGGIFLDPELTTSDRMFRFVYRMFA
                     QGMAAIPNRGMQAIPRQLASHLPQERIRLNAAVEHVWAGGVRLQTGEELFAKAVVVAC
                     DPIHAARLLPQAAIRTTMRGVRCQYFAAADAPIGDRYLVLNGDGEGPINNLCVPSVVA
                     PGYAPPGCHLVSVTVLHTPLSEDHLIRDVREQLIHWFGEQVNGWEYLRTYHIEHALPD
                     QSLEYGGVAMSAVRAESGLYFCGDHCGTASLNGAMLAGRRAADAVCAEFGGPGELF"
     gene            12890..13477
                     /locus_tag="VFC15_06120"
     CDS             12890..13477
                     /locus_tag="VFC15_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09772.1"
                     /translation="MVSKTIEDNLRPYLIGPKLRALRLKKSMGLVELGKHTGLSAALL
                     SKLERDKLYPTLPTLLRIAMVFSVGLDYFFTDERKRHVVAVVRKEERIRLPERPGAND
                     VAYYFESLDFKCNERRTSSYLAEFEPVEAEKLKPHQHPGSEFLHVMKGKLGLKIGLDD
                     FELDAGDSIYFDASVPHTYRRIGKALCQAMIVTAG"
     gene            13608..14789
                     /locus_tag="VFC15_06125"
     CDS             13608..14789
                     /locus_tag="VFC15_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZM09773.1"
                     /translation="MRHISKKLLYAFVLLLLLPSATLLLAQGDSRARDLGIPFDGATG
                     SLNAITDVAGVEVGFTTLISGSGKLVVGQGPVRTGVTAILPRGKQSINDPVFAGWYSL
                     NGNGEMTGTTWVEESGFLEGPVMITNTHSVGVVRDALIKWRFEHGAADLSGYWWSLPV
                     VAETWDGELNDINGFHVKPEDARHALDTAAPGPVAEGNVGGGTGMICNGFKGGTGTAS
                     RKLSAEDGGYTVGVLVQCNYGVQQQLRIAGIAVGRELPPGIPHNDDTGSIIVVVATDA
                     PLLPSQLKRLARRVPLGLGRMGSYSGDGSGDIFIAFSTANPGAWGGGKIKQVNMLPND
                     DMNPLFLATVQSVEESVVNALVAAKTMKGINDFEVDAIPHEELQQALKKYNRLVEPIT
                     K"
BASE COUNT         3205 a         4416 c         4310 g         3083 t
ORIGIN      
        1 cattcttaag cttgacctga gcttcgccca cctcttcgat ttcttctgaa ggtagcagac
       61 gcatcccgta caccaatgtg agctttgcca tgaagtcgat tttaccgccc cttccgaccc
      121 cgtatcgggg aatcgcgcga attgtcactt gttgcagcgg actggttggt gcggttgccg
      181 cgcaaaccgc gagatttccc aattcgacgg cgcttgttag aatccgaagg gcgttagttt
      241 tccgttcaag tgatagcaac gcagccggtt attgttttgc gcgatcttga gtgccacgct
      301 cacgcatctt cctgtattcc cgggaatcta tttctacgtc tatgactgac aaccttcgaa
      361 acgtcattat catcggctcc ggatgctcgg ggctgaccgc cgccatctat gccgcccgcg
      421 ccaatcttcg ccctttggtt atcgacggac atgagcccgg cggccaactc gccatcacta
      481 ctttggtcga gaattttccc ggctttcccg agggtattca ggggccggag ctcatcgaca
      541 atatgcggaa acaggctgcg cgtttcggcg cggagttcac gacggggcat gtggtcagcg
      601 tcgatcttag ccaaaagacc gagggccttg gaacaaaacc catcgaggta agtctgggac
      661 gcgagacctt gctgacccgc gcgctgattg tcgccagcgg tgcctcggcg cgctggctcg
      721 acctgccgaa tgagaaggcc ttgatcggac acggggtctc ttcgtgcgcc acctgtgacg
      781 ggtttttctt ttcgggcaag gagatcgcgg tcatcggtgg tggcgactcc gcgatggaag
      841 aggcgctctt cctgacgcgg tttgcctcaa aggtcacgct cattcatcgt cgcagcgagt
      901 tccgcgcctc aaagatcatg ctcgaccggg ctcgccagca cgacaagatc gagttcctca
      961 ccgacaccgt cgttgacgac gtgtacgacg tcagcaaaaa ggaagtcacc ggacttcggc
     1021 tgcgcaactt gaagactggc gagcaatggg attttcccac cagcgccatg ttcctcggca
     1081 tcgggcatac tccgaatgca gattttgtag gcgatcagct tgctcgcgat ggagaaggct
     1141 acctggttct ttctgatggg catgtctgta ccaaagtccc gggggttttt atttccggag
     1201 acgtggccga ccgtcactat cggcaggcgg tcactgccgc ggggaccggc tgcatggcag
     1261 cgctgcaagt ggagaaatac ctggaagaaa tagggaagtg acaccgatgc accgactctt
     1321 ccggataatg ctgggcggct gacaagcatg ccgaaaccac cttcgaccga atccgacgcc
     1381 gatgaattcc gccgcgccga actaacggcg caattcatcc tgggacgcac cgccttacgc
     1441 ccgcgcgtcg ccctcgtgct gggttcggga cttggcggct tcgccgatga attggagaat
     1501 gcggtccgca ttccgtacgc ggaaattccg tcgttcccgc gcagcactgc gctcggtcat
     1561 gctgggacac tggtcatcgg aactgtttct ggtatcgcag tcgtcgcgat gcagggccgt
     1621 gtacacctgt acgaaggcta ctcggtgcag caggtggcat ttcctgttcg cgtgtttggc
     1681 cggatgggcg tgcgcgctgt cgtgctgacc aatgccgctg gcggcatcaa ccccagctac
     1741 ggacgcgggg cgctggtcgt cattcgcgat cacatcaacc tgcaaggtgg gaatcccctg
     1801 gtgggcgcca acgaggatcg ctttggtccg cggtttcccg acatgacgga tgcctacaat
     1861 gcagagtttc gcaagtttgc actagatgag aaaggtcgca tgggcgacga catttacgag
     1921 ggcatctacg ccgccatgct gggaccgagc tacgaaacgc cggcggagat tcgctttctg
     1981 cgaaccattg gcgccgatct ggtgggcatg tccaccgtgc cggaagtggt tgtcgcccgc
     2041 cacatgggtc ttgaagtctt ggccatttct tgtgtcacca acatggctgc cggaatgagt
     2101 ggcgagaaaa tcaatcacga agaagtgctt gaaaccggcg agcgcgtccg cggtaagttt
     2161 ctggcgctgt tgcgcacggt gttgcccagg atcggtcaat atcttgatgc tcgctccgcc
     2221 tcgttttagt tctagccact acccactagc caccagccac tgatttacac tggacacgca
     2281 tgtccgggaa gtcctatctc attggcattg ccgggccgtc gggagcgggg aagagctatc
     2341 ttgcccacca cctttcccaa cggctgcaag cacccgtgct gccgctcgat cactattacc
     2401 gcgacctctc gcatctgccg ctggaagagc gcgcccgctc caacttcgac gatccggcag
     2461 ctctcgagca cgagttgctg attgagcagg tggcgcaact ctgcgagggg atcgccattg
     2521 aaattccgac ctacgatttt tccgtccata cccggactgg cgagactcag tcgtttcagc
     2581 cggagccctt cgtaatcgtc gaaggtctgt tcaccctgca ctggcctgag ttgcgccgcc
     2641 ttctcggcac gcgagtgttc gttaacatga cagacgacat ttgtctgcag cggcgtaaag
     2701 atcgcgatgt gcgcgagcgc ggacgtaccc cggaatcggt ggtcgaacag tttcgcacca
     2761 cagtcgcccc catggcggaa cgctacgtgc gccccacgca ggctcatgcc cacgtggtcg
     2821 tctttggctc cattcccatt gaggacgaag tcgagcgcgt gctcgaacat gtccggcaaa
     2881 atactcaaga gccaggattg ccaccagctg cgcgtcacat ccggcgttcg ccgtcgcctt
     2941 ccgtaccatc ccgatgagga gtgtcatgca ccgccgccgc atcattgtcg tcttagtggt
     3001 tgtttggctg tgcatcacgt cgcttacggc gcaagttgtc gcgcagaaag cgcagaagga
     3061 agttcccctc tccaccagca agacgcttct ggagccggtt ccgggctcgc cgcagcgcac
     3121 caacagcctc ccagtcacgg ccgctctcag cccggatggt aagtatctcg cactgctcaa
     3181 cgacggattc ggctcggccg agtcggatta tcggcagtcc atctcggtac tggacttgtc
     3241 gagggatcaa ctgcgtgatt tccccgattc acgcctgggg acgctcgctc agcagactta
     3301 cttcctcgga ctcgcctgga gcggcgacgg ccacgaactc ttcgcttcca tggcctccct
     3361 aaccgacccg gaaggcaaga agccgggcaa caccggcaac ggaatcgcgg tgtatcggtt
     3421 cacttatggt gccctgactt ccgacagatt cctcaagctt ccgcttgcgc cgctgggcaa
     3481 gggcaagaag aacgtctacg gatcgaagta tgtgcccgcc ggatttgtgt ctccctatcc
     3541 tgcaggcatt ggcgtgataa gacacgacgg cagaggagcg cttctggtgg ctgagaacct
     3601 ggcggatgac gcggtgctga tcgatgagcg cgatggcaag gtactgcaac gcttcgacct
     3661 cggacgcggc aaggtcgtgc ccagcacatt tccctacacc gtgattgtga atcgcgaggg
     3721 cactcgcggc tggtgctcgc tgtggaatgg ctccagcgta gccgagctcg acctgcgttc
     3781 cggcaaagtc atgcggcaga ttgtcttatt gccaccgaaa gaagagaccg acgcgtcgtc
     3841 gcatcccacg gcattattgc tcagtcctga tgagcgccgg ctctatgtgg cgcttgccaa
     3901 tcgagaccgc gtcgcgatca tcgcgacaac ggacggcaaa atagagcgct acttcgacac
     3961 ccgcctgccg ggacagacgt atggcgggaa ctatccaatc gccctcgcgc agtccattga
     4021 tggcatgcag ctctacgtcg ccgattccag ttctgacgca gtcgctgttt tcgacctgcg
     4081 cgagactcga ggtgggcctg cgaccgccaa tcaggatcgt gcctcgtatt tcatccccac
     4141 cgagtggtat cccacggcgt tggcgctgca agataacgac ctgtttgtcg cgaccggaaa
     4201 gggcatgggc actggtccca actcgggtac tcaggcggtt ccgctgctcg accacaagtc
     4261 gccgcatccc tacattgcct cgttgattcg cggctccatt gcacgcgtca acctgaagga
     4321 cgcggagcgt gatcgcgaag cgctgaccgc agaggtgatg caaagtaatc gcatggaagg
     4381 ccgcactaat cagatcactt ttcagcgcgg cggcaatccc atccaccacg tgatttacat
     4441 catcaaggag aaccgcacgt acgatcagct ctttggcgac atcaaggagg ccaatggcga
     4501 cgcgtcgctg gtaatgtatg gcgatgacat cacgcccaac cagcacaagc tggcgcggca
     4561 attcggggtg ctcgataatt tttacgacag cggagaggtt tccggcgatg ggcacccgtg
     4621 gtcgatggcg gcgatcacga ccgactacaa cgagaaagcg tggcccgtcg catatcgcgg
     4681 tcacgagcgc gaatatgact ccgagggcac catcggcgac gtcgttccgc ttaacgaaaa
     4741 cattcccgac gtaaacgagc cagcgaccgg atacctgttc gacaacctcg cgcgccatcg
     4801 catgagctat cgccactacg gtgagtacat cgaaactcgt tggtgcgagg acctggagca
     4861 gaattacacg ccggccgcaa ccggagcgcc tgccgcgcac gcgccggaat gcgagcgccg
     4921 tgacataaag ccgggcgaag agttgccggc ccagttgggc ggcggcaaga acccgtatca
     4981 gcacgccatt ccattgatgg cctataacat tccggtgaag gcggacctgc gcaatcattt
     5041 cgatccggat tatcccgact tcaaagtcga gttccctgat cagtttcgag tggatatttt
     5101 cctgcgcgag tttacgggat tcgtcgccgc ccgcaagagc ggcaagggag aacagctacc
     5161 cagcttcgcg ctagtgcgcc tgcccaacga ccataccgcg ggcaccaagg ttggctcgcc
     5221 gactccgaat gcgtctgttg ccgacaacga tctggcagtc gggcgcgtgg tcgaggccat
     5281 ctccaacagc ccgtactggg acgacaccgc gatcttcgtg ctggaggacg atgcgcagaa
     5341 cggtgccgac cacgttgacg cccatcgcag cattgggctt gtgatcagca agtattcgcc
     5401 gcaatccccc cagcccttcg tggatcataa cttttacacc acggtgaact tgatgcacac
     5461 catggagacg ctgctcggcc tgcctcccat gaacaacaac gatgctcacg ctgcagtcat
     5521 tgccccactg ttcactggcg caggcgatca gccggcgttc aaggccgagt atcgcaatcg
     5581 cgacaacggc atgatctatc aggccaacac gaaggacgct cccggcgcga aggagtcggc
     5641 ggctctcgat ttctccgtcg ccgatgccgc cgatacggga atactgaacg caatcttgtg
     5701 gcgcgccgcc aaaggtgacg ccgccatgcc cgaaccgcgc catacggtgt ttccagtttc
     5761 tccgggagaa aagtagtggc gtgagtttgc cgttctcacg cgtgttgttg gcaagagaga
     5821 tacttggctc gcgctgtcga acttggacgc ttccgatctc atggattccg tgtaattcga
     5881 cggctgcagc gcacagaagt gccgcttttc gtgaccatct tgggtgatag tgcgaacgac
     5941 ttctgcatta gaatatagag ctgaaggttt gtcagggtgg ggcgccataa gtaacgaaga
     6001 aaaacctaac ggttctggca agcgtgggga acattccagg gacgatgact ccgccgaaaa
     6061 accgggcggc catcgtgccg atcgttcacc ggacaagggc gagcgacagc aagagacgca
     6121 tcctttctcg tctcctactt cgggattcct ggcccaggtg cgaacggctg aaatgctgcg
     6181 cgaagccatt ctgcggcatg tgcgctatac actcgttcgg ccgacttccg accttaaagc
     6241 cgcggattat ctcaagccgg tatcgctagc catacgcgat cgcattgtcg atcgcatgct
     6301 cgaaaccgaa tcccgtttcc gccataagga cgccaagcgt ctgtactacc tgtccatgga
     6361 attcctcatg ggccgtttgc tgggcgacaa cttgtctaac ttgcgcatcg aggagttgtg
     6421 ccgcgaagtg ctggcggggt tcggcatcga tctcgacgat gttctggaga gtgagtctga
     6481 cgccggactt ggcaacggcg gtctcggccg tctcgcggct tgcttcctgg agtcactggc
     6541 cacgctcggc atgccgggtt acggctacgg catcgactat gagttcgggc tgttcaaaca
     6601 ggagatcttc aacggtttcc agcgcgagaa gccggatcgc tggaaagcca acggcacgcc
     6661 cttccagatc gagcaccccg aagaagccat cagcattccc atgtatggcc ggatggactc
     6721 gcagcgtgac gccgacggca atctgaagca gacctggatc aattacaaaa tcgtgctggg
     6781 aattccgacc gacataccca tcgtcggcta tcttggtcag accgttaact ggctgcgctt
     6841 gtttgcggca cgcgcttcgg aagacttcga catcgatatc ttcaatcgcg gcgactatct
     6901 ccgcgccgtg gagcagaaga tcggcagcga gaacatctcc cgcgtgctct atccttccga
     6961 cactgcgatg gcgggcaagg aactgcgcct ggtgcaggag tacttcctgg tggcctgcgc
     7021 cgttggcgat atcatgcgcc gcttccgcca gcagcatgac gatatcgaac tgctgccggc
     7081 caaagccgcc atccagatga acgacacgca tcccagcctt gcggtggcgg aattgatgcg
     7141 cgtcctgctt gaccagtatg gcttgccctt cgacaaggct tgggacatta ctcgccgcac
     7201 cctggcctac accaaccaca cgctcctgcc cgaggccctg gaaaagtggt cggtgccgct
     7261 catggaaaaa gttctgcctc gtcacatcca gatcatcttc agcatcaacc atcggttctt
     7321 gcgcgagatt caactcgagg ctgtcatcga cggggagaag ctgcgcaaga tgtcgatcat
     7381 cgaggaggga tacgagaagc atgttcgcat ggcgcacctg tgcatcgtgg gcagccactc
     7441 ggtgaatgga gtttcagaac tgcatagcaa cctgctggtg aaatcgctgg ttcccgaatt
     7501 cgccgagatt tggcctgaca aattcaataa caagaccaac ggcgtggctc cccgccgctg
     7561 gctgatgaag gccaatccgg ggctcacgga actgatcagc caaaccctcg acgagcagaa
     7621 gtggatcacc gacctgagcc ggttgcgcga tctcgaaccg tttgccgagg acccggaatt
     7681 ccgcgcggcg ttcaaagaag tgaagcggca gaacaaactg aagctggcgg gtgctattca
     7741 agcccaggtc ggggtggtgg tcgatcctga ttccatcttc gatgtgcaga tcaaacgaat
     7801 tcacgaatac aagcgccagc tactggcggt catgaacgtg attcacgact atctgcgtat
     7861 cgtcgaacac gccgagacct tggcatcggc ccggacgttt gtatttgctg gcaaggcagc
     7921 gccaggatac tgggcggcca agcagatcat caagctcatc cataacgtcg cggatgtggt
     7981 gaataacgac gagcgcgtta aggacgccat taaagtgacc ttcctgcccg actaccgcgt
     8041 ttcgctggcg acactgatca ttcccggcgc cgatctgagc gagcagatct ccaccgcggg
     8101 catggaagcc tcgggcacgg gcaatatgaa gctgtccatg aatggcgccc ttaccatcgg
     8161 aacatgggac ggcgccaaca tcgaaatcgc cgaagcggtg ggcctcaaca acatctttat
     8221 cttcgggctg cgtgcggaag agattctgga gatgcagcaa aagggcagct acaatccgcg
     8281 cgagcgttac gacaatgatc ccgacgtgaa ggaagtcatg gacgcgctcg catcagaccg
     8341 cttctgtccc aacgagcatg ggctcttccg ctggatcttt gatgagctgg tccatcgcgg
     8401 cgaccgttac taccacattg ccgacttccc cagttacgtg gagacccaga agcaaatcgc
     8461 ccaggaatat cgccaggacg aaatctggta tcggaaatca attctcaatg tggcgcgcat
     8521 cggaaagttt tccagcgacc gcaccattct cgagtatgcc cgcgatatct ggcatattgg
     8581 gccgtatgaa aaatcgactc ctgttacgaa agccgccggc aaatcccaca cgccggttgc
     8641 gcctctgcct tcggcggtga acggagacgc gatcccagtc gtagagctca ctcaagaagt
     8701 tgagcctaat cccatcaccg agtgagattt agcaagtctt tggaatgacc aatgcggctc
     8761 tcgtgattta tgtcgtgagg tcttcggtgg ccactgcttc gtcggaaccg ccggcaccgc
     8821 tcccctccgg cacttcctcg acgggatagc cgaggtcctt ccaggcatga tatccgccca
     8881 aaagcggccg cacgttgcga ataccgtgtc tccggagttg cagcgccaca cgggcgctgg
     8941 tggcttcgtt gggtcaagta cagaacagca cgatttcccc gccccgggtt atttcgctct
     9001 gcctgctctc cagttcttcc ggcgagatgc gcagggctcc aggcagggtc tgagggtcgg
     9061 gcagcaggtc gagcggatga cgtaaatcaa taatggtcat cggttcaccc gcttccaact
     9121 tctccttgag ctcttcggga gtaatgcgaa accccgcaac ctgtttcagg aagcgatgac
     9181 gttcgtagaa tttgtacgcg acatacccgc cgatcccggc gattaccagc aacagcgtcc
     9241 atgaaccaaa ttgcgctacg tcatgagcga cgcggtcgag ctgccggctg aacagcagtc
     9301 ccagccccgc aaaggcactt gaccacaaca acgctcccat aaagtcgtag aggagaaatt
     9361 gcggcaaccc gatgcctgca gtcgccgaaa gcgcgggagc tgcggtattc agaccgggaa
     9421 cgaacttgca gatcaggagc gtctggagcc cgaaccttgt aaacgtcgac tcggtgcgcc
     9481 gtacgcagga gtcgggctcc aatgcgattt tgcaaaggaa gttgaggacg atgggacctt
     9541 tgcgcttgcc aaaaaagtac caaaacgtgt cgctgaacag cgagccggtc gcggcgacga
     9601 gcaccaccaa ggcgaagttc agttgatgct gaccggccaa ggctccggcc gccagcaaaa
     9661 gtggcatagc tgggatcggc agtcctgcct gttccatcag tacccagaaa aacagcagga
     9721 gatagccgtg ttgcactacg aattgcaggg tttcagccat ggatttgcga ccggaagctt
     9781 aaagacactt cgatgcgcga gaactgcaaa gggttcctgc gaaatgcgtc gatggctggt
     9841 ttgaagaggc gcaactttag ctgcgtcaaa tactgccgct cttctgtttg attcggagtg
     9901 tgagtaagaa ccacgtttgg tttgcgacag tgacacaaat caacaacaaa ccagccccgc
     9961 aggggcgggg acatgatagc ccagcacgta gccgaaggcg gagtgctggg tagcgttgga
    10021 aaaacgatac gagtcccgcg gggacgacag ctttaccact tcagatcgcc agaagcccgc
    10081 cttcttcgca gctactgacg caaactccac cctctcggca tatcgcaaat tccacccctg
    10141 tctaatgcga cgccgcggct tcatactcgg cagccacttc atcaggcttc cattgattgc
    10201 ccgtgtacac tttcgcaatc tttccgtcgg cgccaatcac gacagtttgc agcgtgtgca
    10261 caatctggcc ttgtttctcg ttataggaga gcccaaaaaa gtccgcggcc ttacgaactt
    10321 cctcgggaga tccggaagca aattgccaat ggtggaatcc gggatcgaca tctcccacgt
    10381 acctttttcc gtaactctgc aaaacgatcg gagtgtcgta attaggatcg atgctaatgc
    10441 tcagcaactg ggcctttgcg aatacagcgg ggtttttctg cagctctttt aacacggcgc
    10501 tgaaattgtt gctcatcagc aggcaaaaat caggtactgg gcatcgtgta tagatgaatg
    10561 taagcagcag cggtttaccc cggaactggt cgaggtgaat cgtcttgccg aactgattca
    10621 ccagcgtgaa gtcgggaacg tcgtcgccga tttctgggat ccgcaggtta gaagtaccat
    10681 cgcttccggt gtcgcttccc tgggtgaaag aaatgtcctg cagctctgcg tggtcggtca
    10741 tcaccagggt ggcatgaatg tgatcgccgg ggccgatctt gccgaaaacc cagtcctggt
    10801 cgcgcgcaat cgggaagggc atcgtcatcc ccggcatcag cccgggaatg tcctggtgag
    10861 caacagtgat ttcgcgcgat ccggagtcaa cagccaccac gcgaccttgt aattcgtaac
    10921 gcttgccagc gggcttcttc gagcaggcga tgccggtcag caacaggcca acacacaagc
    10981 acgccacttc caagctccgc cagctatgat ttgctgaaga caggctcgcg attgaaatca
    11041 tgtcaccatg attctatgtg agtcccagtc agcgatgatc ggtatgcacg gaggcgaatt
    11101 gagaaagctc ggaatcgcag ttggtgtcgt gggagttctt tggcttgtct atttctgggt
    11161 ttccgtggct ttatacgcgc acgtcgcaac ggtgatgccg acgttaaaga cggtccaaac
    11221 gttcactcta ggaagtgcgg cagcatgcgc gttttcggga aagactgttt cacgaaaatg
    11281 gtgggccggc gtggccgcgt cgctggtaac gtttgctcta atcatggcga gggttcacta
    11341 gttgccgaaa gcgactagaa caattcgccc ggtccgccaa actccgcaca aaccgcatcc
    11401 gccgctcgcc gccctgccag catggccccg ttcaacgaag ccgtgccgca gtggtccccg
    11461 caaaagtaaa gtccggactc tgctcgcact gccgacatgg caacaccgcc gtattccagc
    11521 gactgatcgg gtagcgcatg ctcgatgtgg tacgtgcgca ggtattccca gccattgacc
    11581 tgctctccaa accaatggat caattgctcg cgcacgtcgc gaatcaggtg gtcttcgctg
    11641 agtggcgtat gcagtaccgt cactgacaca agatggcaac cggggggcgc atacccggga
    11701 gcgactacgc tgggaacgca tagattgttg atcgggcctt cgccatcgcc gttgagcacc
    11761 aggtagcggt cgccgatggg cgcatccgca gccgcaaagt actggcaacg cacaccccgc
    11821 atcgtggtgc gaatagcggc ctgtggcagc agtcgcgccg catggatggg atcacacgcc
    11881 accaccaccg ccttggcaaa cagttcttcg ccagtctgca gtcttacgcc gcctgcccag
    11941 acatgctcga ccgccgcgtt caatcgaatg cgctcctgcg ggaggtgcga agccagttgc
    12001 cgcggaattg cctgcatgcc ccggttcgga atcgctgcca ttccctgggc gaacatgcga
    12061 tagacaaatc gaaacatgcg gtcggaagtt gtcagttcgg ggtccaggaa aattccacca
    12121 agaaaggggc ggaagaaatg ctcgataatc ttcccggaaa atccatactt gcgcagagca
    12181 gcgatggttg aaacttccgg cgcgctgagg acaaggtcaa tctccgtgtc cagaacctct
    12241 ccgcgcaact tcgcgatcag cagtttgtcg ttaaacgtgc cgagtggtgc aaacgcggag
    12301 ggcagaatct ttcgcggccg gcgaatggga tcggatatcg tgtgaaatcg tccgttgtgc
    12361 cgcacgatgg cgcccggctc gaattcgcga agttcgagcg cgccgaagtt gaggcaacgc
    12421 cgagcttcgg gatacgccgt cagcaggacc tggaaaccac ggtccaatag gaacccatcg
    12481 acttcgtcag tgcggatccg tccaccgaca tcgttcgagg cttccacaac ttgaaccgag
    12541 aggccgcgtc gttgtagttc cacggccgct accagtccgg acacaccgga tcccaccacg
    12601 atgacttcgg gatgcatagc aaccaagttt acgcgaagcg gatcatgaaa gagtttgcgg
    12661 tggctggctc cctcgcgcca gccaccgcaa ccaggccgca cgaactttgc gccgaaactt
    12721 gcggcccgcg caatcaagag gccagtgcaa aatcctctag actgcacact tgactataac
    12781 gaaaaactat ttgagtataa agacaagtta aaagtatcga acacttcgtg tgcaatcggc
    12841 gaacaatgct ggcaaatttt ttgccggtaa agaaaaagcg aggttaacca tggttagcaa
    12901 gacgatcgaa gacaatctgc ggccgtacct gatcggcccg aaattgcgtg ccctgcgctt
    12961 gaaaaagagc atggggctgg tggaactagg caagcacacg ggactttccg cagcgcttct
    13021 atccaagctg gagcgcgaca agctgtatcc aactttgcca accctgttgc gaatcgccat
    13081 ggtgttcagc gtcgggctcg actatttctt cactgacgag cgcaagcgtc acgtcgtggc
    13141 cgttgtccgc aaggaagagc gcatcaggtt gcccgaacgt cccggcgcta atgacgtcgc
    13201 atactatttc gagagcctcg acttcaaatg caatgagcgg cgcaccagtt cctatcttgc
    13261 ggagtttgaa ccggtggagg cggagaaatt gaagccgcac cagcaccccg gctcggaatt
    13321 cctgcatgtc atgaagggaa agctgggact gaagatcggg ctcgacgact tcgaactcga
    13381 tgccggcgat tccatttact tcgacgccag cgtgcctcac acctatcgcc gtatcggcaa
    13441 ggcgctctgc caggcaatga tcgttaccgc cggatgagca ttgttgaaca tgccgcaccc
    13501 gagatcgtcg atactcgcgt gcttccttca ggtttcgccg cgaaggaagc tgcccgaaat
    13561 tgctacactt ctgtcgcaca tctaatcaag ttccgaggac gaggtcgatg cgccacatct
    13621 ccaagaaact tctgtacgcg tttgtgcttc tgttgctctt gccctcagca actttgttgt
    13681 tagcgcaggg tgactcgcgc gcccgcgatc ttggcattcc tttcgatggc gcaaccggtt
    13741 cgctgaacgc cattaccgat gttgccggag tcgaggtcgg cttcaccacg ctgatctccg
    13801 gctccggcaa gctcgtcgtg ggccaaggcc cggtgcgcac cggagtgact gccattctcc
    13861 ctcgcggcaa gcagagcatc aacgatcccg tcttcgcagg atggtattcc ctcaacggca
    13921 atggcgagat gaccggaacc acctgggtcg aagagtcggg cttcctcgaa ggcccggtca
    13981 tgatcaccaa cacccacagt gtgggagtag ttcgcgatgc cttgatcaag tggcgcttcg
    14041 agcatggcgc cgccgatctc agtggatact ggtggtcgct gcccgtggtg gcggagacct
    14101 gggatggcga gctcaatgac atcaacggct tccatgtgaa gccggaagac gcgcgccatg
    14161 cgctcgatac tgccgcgcca ggtccggtgg ccgagggcaa tgtcggcggc ggcaccggaa
    14221 tgatctgcaa cggcttcaaa ggcgggaccg gaaccgcgtc gcgcaagctg agcgccgagg
    14281 atggcggcta caccgtcggc gtgctggtgc agtgcaacta cggagtgcag cagcagttgc
    14341 gcattgccgg catcgcggtg ggaagggaat tgcctcctgg cattccgcac aacgacgata
    14401 ccggatcgat catcgtggtg gtggccaccg acgctccgtt gttgccgtcg caattgaagc
    14461 ggctggcgcg gcgcgttccc ctcggtctgg gacgcatggg cagctactcc ggcgacggct
    14521 cgggcgacat cttcatcgcg ttttccaccg ccaatcccgg tgcctgggga ggaggtaaga
    14581 taaagcaggt caatatgtta cccaacgacg acatgaatcc gctgtttctg gcgaccgtgc
    14641 agtcggtgga ggagtcggta gtcaacgccc tagtcgcggc caagaccatg aagggcatca
    14701 acgacttcga agtggacgcc attccgcacg aagaattgca gcaggcgttg aaaaagtaca
    14761 atcgcctggt cgagccgata actaagtagc taagattccc agttgatcat ggcgcgatcc
    14821 ctgaacggcc gggcctctct gcacagtgtc aggaatgccc ccggtctgtt ctgtcaacta
    14881 tcctcccggt ctgtgcctac ccagtgacac cccctcccca cgtatgtttt caatcacttg
    14941 tgaagggttt tccgtgcagc ttcgggcaaa taaatggttt agaggtacct gataggaaac
    15001 attagggtta tggg
//
LOCUS       DASRKK010000278         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_215453, whole genome shotgun sequence.
ACCESSION   DASRKK010000278 DASRKK010000000
VERSION     DASRKK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215453"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFC15_06130"
     CDS             complement(<1..1454)
                     /locus_tag="VFC15_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZM09774.1"
                     /translation="MAPRFVVLLTVSLTLCLPFAVLAQNAKPVPDNPDLIAADQLYKA
                     GKFAEAADKYQALLKIDAKLVAAEAGFIESLLREQKIDEASAEAVKALAAQPNSAALS
                     AAMGDVQLRLAEMPEAEKSYRKALQIDYRELRAYLGLARLYQAYSLYRHSYDSLKAAY
                     QVAPNDPEVQRWWFNRLSRRERIAAIEAYLAGPHPDDPEETEHLRHYLEFLKATVDKP
                     VHACRLVSKVEQTDTKLQPMLRDPRHIVGYGLAVKLNNRNSHLLLDTGASGILVGRKG
                     AEKADLTRISDIRLAGIGDKGLQGGYVAVADHIRVGELEFADCVVTVSDRASVTDEDG
                     LIGADVFSSYLIDIDLPAEKLRLSPLPKRPDETVAPTALNSEGESRSSPDDTAEGAGD
                     QKAESSGDAGKTAKVAPAPRLPKDRYVAPEMKGWTPVFHFGHQLLIPTAVNDSKSMLF
                     LIDTGSSLNLLSTRAGRQVTKVSSDPNVHVKGLSG"
     gene            complement(1575..1856)
                     /locus_tag="VFC15_06135"
     CDS             complement(1575..1856)
                     /locus_tag="VFC15_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09775.1"
                     /translation="MSSRTGFAFARIVLRDGRRANLGAEVIQIYIHGDKVGNEGAIES
                     SGHGKYTWLRAGLGSAVGAGLGGILGGGEGGASAGAASKALSKKQQGEL"
     gene            complement(1925..3706)
                     /locus_tag="VFC15_06140"
     CDS             complement(1925..3706)
                     /locus_tag="VFC15_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM09776.1"
                     /translation="MIRSVIFLLLLSVLSPPAFAQTQPADRSWIATSNRYANMLIDVV
                     FKYRPEAGTQQGLSQFDTKVSQPTLANEDQERHETEAVLAKLKSAAAEKQQQDVAGDL
                     QIMVRRVNLNFKRQDFQRAHEVPFFNASQMVFGGLQVLLDEQTPSDRRAAAVVRIREY
                     AGLEPGYAALTEILKQRVTEQMAKPGVIYPARVEIETEMARNSNYLDGIATLMQKYKL
                     TGWEEPFAKLKTQLTDYDSWVRATVLPKARNDFRLPPQHYALDMENYGIDIPPEQLAA
                     MAHQAFIEIQGEMKSIALKIAKQRNLPSSDYRDVIRELKKQQLVGDAILPFYQERLKQ
                     IEKIIVDQHIVSLPDRAARIRIATAAETAQQPAPHMVPPPFIHNTGQKGEFVLPLNIP
                     AGPGQTSTEKYDDFTYDAAAWTLTAHEARPGHELQFDSMLEHGVSLARVRYAFNSTNV
                     EGWGLYSEYLIKPYMPLEGQLVSLDLRLQRAARAFLDPELQAGKITPADAFRVLEQDV
                     VLSHAFAQEEVERFTYRAPGQANSYFYGFTKLIDLREDTEKALGPKFNEKKFHDFILA
                     QGLLPPDLMRKAVMEDFVPAQKASAKQ"
     gene            complement(4307..>4663)
                     /locus_tag="VFC15_06145"
     CDS             complement(4307..>4663)
                     /locus_tag="VFC15_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09777.1"
                     /translation="GMFELARAYKDTGMTAYSRVQEKEFAREYSHGYSAVKHQRFVGT
                     GYFDEVTQVIASGKSSLSALKHSTEEEQFAEVKVPGQMIEVGQDPSCRPILGECPLTC
                     EHEHEHVRRDNVSAND"
BASE COUNT          884 a         1367 c         1435 g          977 t
ORIGIN      
        1 ccgctgagtc cctttacgtg cacattgggg tcggagctga ccttagtgac ttggcgaccg
       61 gcccgtgtgg agagaaggtt gagcgacgag cctgtatcga ttagaaacag catcgacttt
      121 gagtcattga ccgcggttgg aattaggagt tgatggccga agtggaagac aggagtccag
      181 cctttcatct ccggagcgac gtagcggtct ttcggaaggc gaggcgcggg ggcgaccttg
      241 gcggtcttgc cggcatcgcc gctcgattcc gccttctgat cgccagcgcc ttcggcggta
      301 tcgtcggggc tcgagcggga ctcgccttcg ctgttgaggg ccgtgggtgc gaccgtctca
      361 tccggccgct tgggaagcgg cgacaacctt agcttctccg ccgggagatc gatgtcgatc
      421 agataggagc tgaagacatc cgcgccgatc aggccgtctt cgtcggtgac ggacgcccga
      481 tcgctgacgg tgactacaca gtctgcaaac tccagttcgc cgactcgaat gtgatcggct
      541 actgccacgt atccaccttg cagaccctta tcgccgattc cggcaagacg gatgtcggag
      601 atgcgcgtca ggtcagcttt ctccgcgcct ttacgaccga cgagaattcc gctggctccg
      661 gtatccagca gcaggtggct gttgcgattg ttgagcttga ccgccagacc atagccgacg
      721 atgtgtcgcg gatcgcgtaa catcggttgc agcttggtgt cggtttgctc caccttgctg
      781 accagtcggc aggcatgcac gggtttgtca accgtggcct tgaggaactc gaggtagtgt
      841 cgcaggtgct cggtttcttc cgggtcgtcc ggatgcgggc cggcgagata cgcctcgatg
      901 gctgcgatgc gctcgcggcg cgaaagcctg ttgaaccacc agcgctggac ttcgggatcg
      961 ttcggagcaa cctgatacgc cgccttcaac gaatcgtagc tatggcgata gagcgaatag
     1021 gcttgataga gacgagccag accaagatag gcacggagtt cccgatagtc gatttgcagt
     1081 gctttgcggt acgacttctc cgcttcaggc atttcggcca ggcgaagctg cacgtcaccc
     1141 atggccgccg atagcgccgc ggaattgggt tgagcggcca gcgccttgac ggcctcggcg
     1201 gaagcctcat cgatcttctg ctcgcgcagt aatgactcga tgaatccggc ttctgccgct
     1261 accagcttgg cgtcgatctt cagaagcgct tgatacttgt ctgccgcctc cgcgaacttg
     1321 ccggctttgt aaagctgatc ggcagctatc aggtcaggat tgtcgggaac cggcttggca
     1381 ttctgggcga gcacggcaaa gggaaggcag agcgtgagag agacggtaag taagacgacg
     1441 aagcgtggtg ccatgagggg ccactcctgc ttgaaagcag agtatcaccc atggaaagcg
     1501 aactggccac ggattgttcg ggagacaact gagcgcggat ttattctgga atcggagtga
     1561 ggcgcaaatg ccgctcacaa ttcgccttgc tgctttttgc tgagggcttt ggaagcggct
     1621 cccgcgctgg cgccgccttc gcctccacca aggattccgc ccagcccagc gccaaccgct
     1681 gagcccagcc ccgcgcgcag ccaggtgtac ttaccgtgcc ccgagctttc gatagcgcct
     1741 tcgtttccga ctttgtcgcc atggatgtaa atctgtatga cctcggcgcc aagattggcg
     1801 cggcggccat cacgaagaac gatgcgggcg aaggcgaagc ccgttcggga tgacaaaata
     1861 aggagcttta ggggcgcacc tagaaggtgc gccccttcaa aacaccattg ggacgcaccc
     1921 gcaactattg cttcgcactt gccttctgtg cgggcacaaa atcctccatc accgccttgc
     1981 gcatcaggtc gggtggcagc aggccttgcg ccaggatgaa gtcgtggaac ttcttctcgt
     2041 tgaacttggg ccccagcgct ttctccgtat cctcacgcag atcaatcaac ttggtgaatc
     2101 cgtagaaata gctgttcgcc tgtccgggcg cgcgataggt aaagcgttcg acctcctcct
     2161 gcgcaaaggc gtgcgatagc actacatcct gctccaggac gcggaaggca tccgctggcg
     2221 tgattttgcc ggcctgtagc tcggggtcga gaaacgctcg cgcggcccgt tgcagcctca
     2281 ggtccagcga gaccaactgg ccttccagcg gcatgtacgg cttgatgaga tattccgaat
     2341 acagtcccca gccttcgacg ttagtgctgt tgaacgcgta gcgaacccgg gccagcgaga
     2401 ccccgtgctc cagcatggag tcgaactgca actcatgtcc cggccgtgcc tcgtgcgcgg
     2461 tcagcgtcca ggcggcggca tcgtaggtga agtcgtcata cttctcggtt gaggtctgtc
     2521 ccgggccagc cggtatgttc aggggcagaa cgaactcgcc cttctgcccg gtgttgtgga
     2581 tgaacggtgg aggcaccatg tgcggtgcag gctgctgcgc ggtctccgcc gccgtcgcga
     2641 ttcgaatgcg ggcggcccgg tcgggcagcg acacgatgtg ctggtcgacg atgatcttct
     2701 cgatctgctt caggcgctcc tggtaaaacg gcaggatggc gtcacctacg agttgctgtt
     2761 tcttcaactc gcggatcacg tcgcgataat cgctcgacgg caggttgcgc tgcttggcaa
     2821 tcttcagcgc aatggacttc atctctccct ggatctcgat gaaagcctgg tgcgccatgg
     2881 cagcgagctg ctccggcgga atgtcgatgc cgtagttctc catgtcgagc gcatagtgct
     2941 ggggcggaag acgaaaatcg ttgcgcgcct tgggcagcac cgttgctctc acccacgagt
     3001 catagtcggt aagttgcgtt ttcagcttgg cgaacggctc ttcccatccc gtcagtttgt
     3061 acttctgcat caacgtggca attccatcca gatagttgga attgcgcgcc atctcggttt
     3121 caatctcaac ccgcgcggga tagatcacgc ccggcttggc catctgctcg gtgacgcgct
     3181 gcttcaggat ctcggtgagc gccgcataac cgggctccag tccggcgtat tcgcggatgc
     3241 gcaccaccgc agccgcccgg cggtcggaag gtgtctgctc gtcgagcaaa acctgtagcc
     3301 cgccgaagac catctggctg gcgttgaaga acggcacttc gtgggcgcgc tggaaatcct
     3361 ggcgcttgaa gttgaggttg accctgcgga ccatgatctg gaggtccccc gcgacgtcct
     3421 gctgctgctt ttccgccgcc gcgctcttca gcttggcgag caccgcctcg gtctcgtgcc
     3481 gctcctggtc ctcattggcc agcgtgggtt gcgaaacctt ggtgtcgaac tggctcaacc
     3541 cctgctgcgt gcccgcctca ggacggtact tgaagacgac gtcgatcagc atgttggcgt
     3601 agcggttgct ggtggcgatc caactgcgat cggcaggctg ggtttgcgcg aaagccggcg
     3661 ggctaagaac ggacagtaat aaaaggaaga tcacgctgcg aatcaaatcg tcctccgggc
     3721 gaagcaatct aagtcgtcaa agtatagatg ggattttggg gaggcgaaag gcgcaggccg
     3781 aatcgagacc gaatcgaagg acctggtagg gtgctgtccc tgcgggatac ggacttcctc
     3841 tggaccagcg atgacatctc cggtaaacgt tgcgatgagg aggcaagtgg aaaggaaagc
     3901 ttggccacgc actaatgaga agaaaggacc gcagatcccc tcggcttcgc tcggggcagg
     3961 cacttcgact gcgcgccggc acgacccgca caatcacccc aacgatcgca aaggcggcgc
     4021 tcgttgggga ccccgaaaag aagcgggtcg gaaattcttg gcgggcgttg cgctccgtcg
     4081 aaaatcattc accacggaga cacggagggc acggagtttt agcttcttta gaggtttctc
     4141 agtgacctcc gtgcctccgt ggttaatttt catttctatg ggtgggccgc agacccatgc
     4201 gatactcagg atgacagtct taaggccctc aaggaaaatc aggcacggga ttggataacc
     4261 ccgtgcctgt atgcgtagta cacgctctcc ctgccgagag aaacttctaa tcgttggctg
     4321 agacgttgtc gcgccgaacg tgctcgtgtt cgtgctcgca cgtcagcgga cactcgccca
     4381 ggatcggacg gcaggacgga tcctgtccca cctcgatcat ctgccccggg accttgacct
     4441 ccgcgaattg ctcctcctcg gtcgagtgct tcagcgccga caacgacgac ttgccgctgg
     4501 cgatgacctg ggtgacctcg tcgaagtagc cggtgccaac gaagcgctgg tgcttcaccg
     4561 cggaataacc gtggctgtat tcgcgggcga attccttctc ctgcacgcgc gagtaggcgg
     4621 tcatgcccgt atctttgtag gcgcgcgcca actcgaacat ccc
//
LOCUS       DASRKK010000279         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_215533, whole genome shotgun sequence.
ACCESSION   DASRKK010000279 DASRKK010000000
VERSION     DASRKK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215533"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1549
                     /locus_tag="VFC15_06150"
     CDS             197..1549
                     /locus_tag="VFC15_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM09778.1"
                     /translation="MVFCAAQQNIEPAKKQESIQAAGQGAAAKAAPAITYIRAGKLFD
                     STSDALRENVVIVVEGERIKAVEPAANVQIPAGAKVIDLSKSTVLPGLIDCHTHLGAR
                     ADRYNPINAFKETPYTEGFAAEKNARTTLMAGFTTVRDVGSGPFMAVDLRNSINEGYI
                     VGPRVVASGPGISITGGHGDLNNYSPETSVSLFPAERDYQLADGADQVRHVVRAQVKY
                     GVDVIKIMATGGVLSKGDSAGAPQFTYEELKVAADEAHMAGRKIAAHAHGTQGIKNAI
                     LAGIDSIEHASIADDEDIQLAKQHGTYFDMDIYNDDYLLGKAIEFGLPQENVDKEKMV
                     GRAQRETFQKAVQAGVKMGFGTDAGVYPHGENAKQFVTMVKFGMTAPQAIRTATQNPA
                     DLIGRSKDVGTIEVGKYADIIAVSGDPLQDVSILQNVGFVMKGGVVYKDKLAGTAVAE
                     "
     gene            complement(1833..2384)
                     /locus_tag="VFC15_06155"
     CDS             complement(1833..2384)
                     /locus_tag="VFC15_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZM09779.1"
                     /translation="MTHAWHDISPGEDLPQEFLTVIEIPFGSSVKYELDKKSGLIKLD
                     RVLYSAVYYPANYGFIPQTLAEDDDPLDVLVLCQEAVVPLTIIHARAIGLMTMIDAGK
                     KDHKIIAVATQDPEFNSYREAGEMPEHRLSMLRRFFLDYKQLEGKAVEVDEISPAQHT
                     YPIIEDALARYSQQRRKGFGTSH"
     gene            complement(2912..3253)
                     /locus_tag="VFC15_06160"
     CDS             complement(2912..3253)
                     /locus_tag="VFC15_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09780.1"
                     /translation="MKILSLSSDPELSELRRAILEAAGHQVHSLTSDKEALQAAQGPE
                     PYDVVLLCHRLPTSLARQVFRLLRENHPDTRIIYIVHVYGEWPEVEADRYVVGADGPD
                     ALVRVLEEVQV"
     gene            3593..4471
                     /locus_tag="VFC15_06165"
     CDS             3593..4471
                     /locus_tag="VFC15_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HZM09781.1"
                     /translation="MPQKEKWDPEMAEFQSQKKRFILASDFDQTLSFNDSGFVLSELL
                     GIPSSEYERKITGMAKLNLVQQGGELAYLLLHDPEFRKVRREHLYEAGKRVRLKPNID
                     ALYEFLSNEVEGFHFDFYVISAGPVEIIRSALEGIIPPDHIYGTEFEFNAKGEIERIL
                     HVTAGYGKVAAVDNLLEELKVGPDRLVYVGDGSSDIHVMLHVNRRDGYTIAVSENKYL
                     APIAKRSIISDNALAILIPILEDVAGWDSMAIRSVFEAKGFLIQQWDKVQTDLLTIIP
                     SSNIPAMEPEGVVATE"
     gene            complement(4531..>6269)
                     /locus_tag="VFC15_06170"
     CDS             complement(4531..>6269)
                     /locus_tag="VFC15_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM09782.1"
                     /translation="DTLAQQKPDFEQTMAKRPVRFYDTNLFATTGQMLLPPQVLREAN
                     SKLLYGIKEIMPLPVTAVKVGSEWDVGVFGLRMKLERFEPVGTESCAVFVDTGTRKDT
                     HLQFTFCPESGSLAKLAFEGQYLELDGPIHEKVSLELREVHHHETPPAWMTDPQIQLA
                     ALIAFVAAKTPLPDASGMDAILKAGSPEAQALVLAAYYQRAVAPTPAILQPLLQSNDA
                     EVRRIAGRFNESPPKPASQPCDLPAAHHSREKPGTTLRGMSSPGFAGAPYMIHVPLDY
                     RGDQPFPLVVYLSGGGGLAFDAALTAGEAIKHAGYLVLYPHAGGDLWWEHKRTEMTHA
                     LLLEVLRNYNVDSNRVYLAGFSNGGTGALEFGARWPDRFAAIASLMGAGLDSPRGVKL
                     PMSNLLDVPVLFLHGDQDPRIPSSASVKTYDELRTLKPRVEPELHILKGRAHEVTLAS
                     DDGFTLPFFERFTRNPFPQNISARVWDTRFPRQYWIEVVEGDTAPSEVEAHITADNLI
                     DIKTRDVKKLRLLLRPELFHSTGPVHIRLNGNDHPALELKQDCQLFLRSADAYADHFL
                     AYTDEVVLDVPK"
BASE COUNT         1399 a         1613 c         1960 g         1297 t
ORIGIN      
        1 tttcacggat ctccacggac aatactggag cggaaaacag ggccaccgcg gattgcgcgg
       61 aaaacgcgga tcggattcaa ttcagaagag cgaaaaaagt aaccgggatg gaatcgcgag
      121 actggttttg aattcttgtt atattgaata catagctttc agacgtggga gacaccgatt
      181 tgagacacaa gattctttgg ttttttgcgc ggcacaacaa aacattgagc ccgccaagaa
      241 gcaggaaagc atacaagctg ctggtcaggg agctgctgca aaggccgcgc ctgcgatcac
      301 ctacattcgc gccggcaagc tgttcgattc caccagcgat gctctgcgcg agaacgtagt
      361 gatcgtggtg gagggtgaac ggatcaaggc ggttgaacca gccgcgaacg ttcaaatccc
      421 tgcgggagcg aaggtgatcg atctgtcgaa gtccacggtg ctgccgggcc tgatcgattg
      481 ccacactcac ctgggcgcac gcgcagatcg ctacaatccg atcaacgcct ttaaggaaac
      541 gccgtatacc gaaggtttcg cggcggagaa gaacgcgcgc accacgctga tggcgggctt
      601 tactacagtg cgcgacgtag gatctggtcc tttcatggcc gtggatttgc gcaacagcat
      661 caacgaggga tacattgtcg gcccgagggt ggtggcaagc ggtccgggga tttcgatcac
      721 tggcggacac ggcgatttga acaactattc gccggagacc agtgtgagcc tgtttccggc
      781 ggagcgtgat taccaactcg ccgacggcgc cgaccaggtg cggcatgtgg tgcgcgcaca
      841 ggtgaagtat ggcgtggacg tgatcaagat catggccacc ggcggagtgc tgtcgaaggg
      901 cgacagtgcg ggagcgcctc agttcaccta cgaagaacta aaggttgcgg ccgacgaggc
      961 gcacatggcg ggacgcaaga ttgccgcgca cgcgcacgga acgcaaggca tcaagaacgc
     1021 gattcttgcg ggcatcgatt caatcgagca cgctagcata gccgatgacg aagatatcca
     1081 actggccaag caacatggca cgtacttcga catggacatc tacaacgacg actacctgct
     1141 gggcaaagcg attgagttcg gattgccgca ggaaaatgtc gacaaagaaa agatggttgg
     1201 ccgcgcacaa cgcgagactt ttcagaaggc ggtgcaagcg ggcgtgaaga tggggtttgg
     1261 cacggatgcg ggagtctatc cacacggcga gaacgctaaa cagttcgtga ccatggttaa
     1321 gtttgggatg actgctccac aggcgatccg cacggctacg cagaatcccg ccgatttgat
     1381 tggccgctcg aaggatgtgg gcacgatcga agttgggaag tatgccgaca tcattgcggt
     1441 gtcgggcgat ccgttgcagg acgtgtctat cctgcagaat gtcggattcg tgatgaaggg
     1501 cggagtggtt tacaaagaca agctggcggg tacggcggtc gcggagtaga gcgggttcct
     1561 cgtgtgggtg agaatatctg ccgtccccgg gggactcgat tctctttcca cctttaccca
     1621 gcactccgcc tgcggctgcg tgctgggcta gactatgcct cccctacggg gatggctttt
     1681 ctggtcgatt cgtaccacta tcgcagccca aacgacccag acttcacgcg cacaatcaag
     1741 ctcgcgactg tgacgggtat attggccgcg tacggcgctg gagcgtcgca ctatccggcc
     1801 gggcaccgaa tcacagtact tctaaaacga atttaatgcg acgtcccgaa gcccttgcgg
     1861 cgttgctgac tgtagcgggc gagcgcgtct tcgatgatgg gataggtgtg ttgcgcgggc
     1921 gaaatttcgt caacctcaac ggctttgcct tcgagctgtt tgtagtcgag aaagaaacgc
     1981 cgcaacatgc tcaagcgatg ctcgggcatc tcgccagcct cgcgatacga attgaattcc
     2041 gggtcctggg tggcgacggc gataattttg tggtccttct tgccggcgtc gatcatggtc
     2101 ataaggccaa tagcccgggc atggatgatg gtgagcggta cgacggcttc ctggcacaaa
     2161 accaaaacgt cgagcgggtc atcgtcttcg gcgagagttt gtgggatgaa tccgtagttg
     2221 gcgggatagt agaccgccga gtagagcacg cgatccaact tgatcagccc gctcttcttg
     2281 tcgagttcgt acttaacgct cgatccgaaa gggatttcga ttacagtgag aaactcctgc
     2341 ggcagatctt cgccgggaga gatgtcgtgc caggcatgag tcatgagcgt tcaactcttt
     2401 cgcagagagt aaggcaagcc acaacacatt gtatcggaga ggtgcgggag aaagtttaga
     2461 ggccgatcgg gggacggtgt ccctcgcgag gacgggctgg gcccagtgtt tgcgacgccg
     2521 ggagcagcct gtgaagccat gctccttcaa aaccgactag tgaaaccggt tctaacggta
     2581 ttggtggggc aattcagttt cggtttccac agaactggtc agcgaaccga cagcataagc
     2641 aattacgatt acaaccaata ccgctaccgt tacgacatcc atggcgcacc tcaccccagg
     2701 ccagcaatct gaatcgttca atcagtccga gtcagtcgcg gatcctttgt tcaatacttc
     2761 ctaccccaag cggaaaagaa agttgtggat tgtttgtagc acagtttcga cagcgatggg
     2821 ggtgcgatct tgagtacaag ctgaaatgcg gagccccgga ggctgcccca tcttgatcct
     2881 gcggcagcat ggtgagcgtg gcggaaggcg cttacacctg aacctcttcc agaacgcgaa
     2941 ccagggcgtc gggtccgtcg gcgcctacga cgtagcggtc ggcctcaact tcaggccatt
     3001 cgccatagac gtggacgatg tagatgatgc gggtatcggg atgattctcg cgcagcaggc
     3061 gaaagacctg gcgcgccaag gacgtcggca aacgatggca gaggaggacg acgtcgtacg
     3121 gctcgggacc ctgggctgct tggagcgctt ccttgtctga ggttagggag tggacttgat
     3181 gtccggcggc ttcaaggatg gcccggcgca gctcagagag ctcggggtcg gaactgagcg
     3241 agaggatctt catggtagga caatcctgat gtatggtgtg gagcggcgca gtcgcaggat
     3301 aacagaatcg gaggcaacta cgggggtgag gacatttcgt gcgaggcgtt gcggcgggtg
     3361 tcgacaaaca ggtattgagg ggcagcgata ttcgtattgc attcgctttc gtgtatcagc
     3421 taaaacattg taaagcgctg taaataagcc gtttgctcaa taaacgattt caatcgccgg
     3481 tatccaaaga atctatccga attcatcatc tttcaatcct cggaaccatc caataggtac
     3541 ataatcgttt cacgtcttca taacctaacc aaattattaa ggagggcttt ttatgcctca
     3601 gaaagaaaaa tgggacccag aaatggcaga atttcagtca caaaagaagc gattcatcct
     3661 ggccagcgat tttgatcaga cgctgagttt taatgactcc ggatttgtcc tgagcgagct
     3721 gctgggaatt ccgtcgagcg agtacgagcg caagattacg gggatggcca agctgaacct
     3781 ggtgcaacag ggaggcgagt tggcctatct gctgctacac gatccggaat tccgcaaggt
     3841 gcggcgcgag catttgtacg aagcgggcaa gcgggtgcgg ctcaagccga atatcgatgc
     3901 gttgtacgaa ttcctgtcga acgaggtcga gggctttcat ttcgacttct atgtgatttc
     3961 ggctgggccg gtggaaatca ttcggtcggc gctcgagggc attattcctc ctgaccatat
     4021 ttacgggacg gagtttgagt tcaatgccaa gggcgagatc gagcgcattc tgcacgtaac
     4081 ggcgggctat ggcaaggtgg ccgcggtcga taacctgctg gaagaattga aggtcgggcc
     4141 cgaccgcctg gtgtatgtcg gcgacggcag ctcagacatt cacgtgatgc tgcacgtcaa
     4201 tcggcgtgac ggttacacga tcgcggtgtc ggagaacaag tacctggccc cgatcgcgaa
     4261 aaggtcgatc atcagcgaca acgcgctggc gatcctgatt ccgatactag aagacgtggc
     4321 gggatgggac agcatggcga ttcgcagcgt gtttgaagcc aagggattcc tgatccagca
     4381 gtgggacaag gtgcaaaccg acctgctgac catcattccc agcagcaaca tcccggcgat
     4441 ggagcccgag ggagttgtcg cgacagagta acggaatacc ccgtcgagaa ttgtgcccgc
     4501 tgcccttcgg ggtggcgggc tttttgttgc ttatttgggg acatcgagga cgacttcgtc
     4561 ggtgtaggcg agaaagtgat cggcgtaggc gtcggcactt cgaagaaaaa gctggcaatc
     4621 ctgcttgagt tcgagtgctg ggtgatcatt gccattcagc cgaatgtgga ctgggccggt
     4681 ggagtggaaa agctcaggac gcaacagcaa gcgcagcttc ttcacgtcgc gagtcttgat
     4741 gtcgatcagg ttgtcggcgg tgatgtgggc ttcgacttct gacggcgcgg tatcgccctc
     4801 gactacttcg atccagtatt gccggggaaa tctggtgtcc cagactctag cggaaatatt
     4861 ttgcgggaat gggttgcggg tgaatcgttc gaagaacggc agggtaaatc cgtcatcgga
     4921 cgcaagagtg acctcgtgtg cccgcccttt caagatgtgc aattccggtt cgacacgtgg
     4981 cttgagggtg cgcaactcgt cgtaggtctt gacggatgca gaggacggga tgcgcggatc
     5041 ctgatcgccg tgcagaaaca acaccgggac atcgagcaga ttgctcatgg gcagcttcac
     5101 ccctctggga gagtccaggc ccgcacccat cagagaagcg atggccgcga agcgatcggg
     5161 ccagcgggca ccaaactcta gcgcgccggt gccaccattg ctgaatcctg cgagatacac
     5221 gcggttgctg tcgacgttgt agttccgcag tacttccagc agcagggcgt gcgtcatctc
     5281 ggtgcgcttg tgctcccacc acagatcacc tccggcgtga ggatagagca cgagataacc
     5341 ggcatgtttg attgcttctc ctgcggttag cgcggcatca aaggccagac caccgcctcc
     5401 gctgagatag acaaccagcg ggaagggctg gtcaccgcga tagtcgagcg gaacgtgaat
     5461 catgtagggc gcacccgcaa agccagggct gctcatgcca cggagagtgg tacccggctt
     5521 ctcgcggctg tggtgcgcgg ctggtaggtc acagggctgg ctggcaggct tcggcggcga
     5581 ctcattgaag cgaccggcga tacgccgaac ttcggcgtcg ttgctctgca ggagaggttg
     5641 aagaatcgcc ggagtgggcg cgactgctct ttgatagtaa gcggcgagca cgagagcctg
     5701 ggcctcgggc gatccggctt tgaggatcgc gtccatgccc gaagcgtcgg gcagaggagt
     5761 tttagcggcg acgaaggcga tcaatgctgc cagttgaatc tgcgggtccg tcatccacgc
     5821 cggcggagtc tcatggtgat ggacctcgcg caattcgagg ctgacctttt cgtgaatcgg
     5881 gccgtcgagt tcgagatact ggccttcgaa ggcgagcttc gccagacttc ctgattcagg
     5941 gcaaaaagtg aactgcaggt gagtatcttt gcgcgttccg gtgtcaacaa acacggcgca
     6001 cgattccgtg ccgaccggct cgaagcgctc cagcttcatg cgcagcccaa acactcccac
     6061 gtcccattcg gagcccacct ttactgcagt caccggcaga ggcatgatct ctttgatgcc
     6121 atagagcagc ttgctgttgg cttcgcgcaa gacctgcggc ggcaacagca tttggccggt
     6181 ggtggcgaag aggttcgtat cgtagaagcg gacgggacgc ttggccatcg tctgctcaaa
     6241 gtccggcttc tgttgggcaa gagtgtctt
//
LOCUS       DASRKK010000280         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1291326, whole genome shotgun sequence.
ACCESSION   DASRKK010000280 DASRKK010000000
VERSION     DASRKK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291326"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /gene="glgX"
                     /locus_tag="VFC15_06175"
     CDS             complement(<1..1694)
                     /gene="glgX"
                     /locus_tag="VFC15_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZM09783.1"
                     /translation="MPPKSLTRSQPQKSTTTSVAGYRVNRGVALPLGATLRRTGVNFA
                     IFSKHATSCTLVLLQPGAEHPFVEFPLDPRSNRTGQVWHVFVEGLDAGMQYGYRFDMQ
                     PNPNPNVHRFNPELVLLDPYARVLSNGGAWSRYPPGKRPYRNSVAVENQFDWEDDQPL
                     NIPLVNSIVYEAHVRSFTVHSSSAVAHPGTYAGLIEKIPYFQKLGVTAVELLPVNEFE
                     QGDTDRVNPFTGERLLNLWGYQPTAFCAPNTAYSSQRADGEQVHEFKQLVRTMHQAGI
                     EVILDMVFNHTAEGDETGPTWSFRGIDNSTYYILDPETGKYVNFTGCGNTVNCNNPVV
                     RDFIVDCLRYWVLEMHVDGFRFDLASILGRGMDGRVLKNPPLLESLAYDPVLANTKLI
                     AEAWDAAGLYQVGTLPSWGRWAEWNGIFRDDVRKFVKGDAGMTSALATRLLGSPDLYE
                     TSAREPYHSINFLTCHDGFTLNDLVSYNQKHNLANGDNNTDGSDANYSWNCGVEGPTN
                     DAAVNELRARQRKNFAAILLFSHGVPMILAGDEIARTQQGNNNAYCQDNEVSWLDWR"
     gene            complement(1914..>2109)
                     /locus_tag="VFC15_06180"
     CDS             complement(1914..>2109)
                     /locus_tag="VFC15_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09784.1"
                     /translation="PGIDTMVAAFTTDIPALTNWGKPLLIGPGSIHVAHTEGEYIEKR
                     QLLEAIDLYARIARELLSNQ"
BASE COUNT          417 a          581 c          678 g          433 t
ORIGIN      
        1 cgccagtcca gccagctcac ttcgttgtcc tgacagtagg cgttgttgtt gccctgctgc
       61 gtgcgtgcaa tttcgtcgcc cgcgagaatc atgggaacgc cgtgcgagaa cagcaaaatt
      121 gcggcaaagt tcttgcgttg acgcgcgcgc agttcgttaa ccgcggcatc gttcgtgggg
      181 ccctcgacgc cgcaattcca actgtagttg gcgtcggagc cgtcggtgtt gttgtcgccg
      241 ttggcgagat tgtgcttctg gttgtaggaa accaaatcat tcaacgtgaa gccatcgtgg
      301 caggtcagga agttgatgct gtggtaaggt tcgcgggcgc tggtctcgta aaggtcggga
      361 cttcccagca accgggtcgc gagtgccgag gtcatgccgg catcgccctt cacgaacttg
      421 cgcacatcgt cgcgaaagat gccgttccac tccgcccatc gtccccagga aggaagcgtg
      481 ccgacttggt acagcccggc ggcatcccag gcttcggcga tcagtttggt attagcgagc
      541 accggatcgt aggccagact ttccaacagc ggtggattct tgagcacccg cccatccatt
      601 ccgcggccga gaatggaagc gagatcgaag cggaagccgt ccacgtgcat ttccaggacc
      661 cagtaacgca ggcaatcgac gatgaagtcg cgcaccacgg gattgttgca attcacggtg
      721 ttgccgcaac ccgtgaagtt cacatacttg ccggtctcgg ggtcgagaat gtaataggtg
      781 gagttgtcga tgccgcggaa ggaccacgtc gggccggtct cgtcgccttc ggcggtgtgg
      841 ttgaacacca tgtcgagaat gacctcgatg ccggcttggt gcatagtgcg caccagttgc
      901 ttgaactcgt gcacctgttc gccatcggcg cgctgcgatg agtaagcggt gttgggcgcg
      961 caaaacgccg tcggctgata accccacaga ttcagcagac gctcgccggt aaacgggttg
     1021 acacggtcgg tgtcgccttg ctcgaactcg tttacgggga gcagctcgac cgctgtcact
     1081 cccagtttct ggaagtaggg gatcttttcg atgagtccgg cataggttcc gggatgcgcg
     1141 accgcggaag aagaatgtac ggtgaagctg cggacgtggg cttcgtagac gatgctgttc
     1201 accaggggaa tgttgagcgg ctgatcgtct tcccaatcga attggttttc caccgccaca
     1261 ctgttgcggt aaggccgctt ccccggcgga taccggctcc atgcaccgcc gttggagagc
     1321 acacgcgcgt agggatcgag cagcacgagc tccgggttga agcgatgcac attgggattc
     1381 ggattgggct gcatgtcgaa gcggtaacca tattgcatgc cggcgtcgag cccttccacg
     1441 aaaacatgcc acacttgccc ggtgcggttg gagcgtggat cgagggggaa ctccacaaag
     1501 ggatgctccg ctcccggctg caagagaacc agcgtgcaag acgtcgcgtg cttcgagaag
     1561 atggcaaagt tgaccccagt gcggcgcaag gttgcgccca ggggaagcgc aactccgcgg
     1621 ttgacgcgat aaccagcgac cgaagtggtg gtagatttct gcggctggct tcgggtaagc
     1681 gatttaggcg gcatggcgtc gtgaccccga cgggccaaca ggatggatag cagaaagtag
     1741 gaagaacagc aaggaaaagc tgcagttcta atgcagttct attcgtggca gcttacccag
     1801 cactcacgtg ccgggctagg attgttccgc cctgcaggct ggggttcttg ccatgtacgc
     1861 ccttttagcg aagccagggc ggggcggaat catcggcccg agcaacgagt gtttcactgg
     1921 ttcgaaagca gctcgcgcgc gatgcgcgcg tatagatcaa tcgcctccag caattgccgc
     1981 ttctcgatgt actcgccttc ggtgtgggcc acgtggattg agccgggacc aatgagcagc
     2041 ggcttgcccc agttcgtcag cgcgggaatg tcggtggtga aggcggcaac catggtgtcg
     2101 attccgggc
//
LOCUS       DASRKK010000281         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_20992, whole genome shotgun sequence.
ACCESSION   DASRKK010000281 DASRKK010000000
VERSION     DASRKK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20992"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..950
                     /locus_tag="VFC15_06185"
     CDS             549..950
                     /locus_tag="VFC15_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09785.1"
                     /translation="MPYIQWLFIDIKHMDSGKHKEGTGVPNELILDNIRWIRRTNWPG
                     RMVVRMPVVPGFNDTVANAEATAEFLREIGLSEINLLPFHRLGASKYEQLGMTYQYVE
                     QATVAQESLETLAMVYREKAITCHSGADTPF"
     gene            1767..2420
                     /locus_tag="VFC15_06190"
     CDS             1767..2420
                     /locus_tag="VFC15_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09786.1"
                     /translation="MRKAVQNPAASLISVPLQNNTNFDIGPYDRTQNVLNIQPVIPVH
                     ITKNWNLITRIIQPIVWQPYPDQSTGGELGFGDMNPTFFLSLFKPGKLIWGAGPTASG
                     LRYTQFHTTALLLPHACGAPRWSQPPFQRHRRHHGTRHGLPGLQLADAQGQRNICRSA
                     QAERGSSGVRNRMPCHVETQNLATVMADYEEAVQHSECEADISARCRASSHPFCRRS"
BASE COUNT          563 a          766 c          709 g          549 t
ORIGIN      
        1 atcggcgaga tgcaggagca gccgctcaag acctgcggtt ctgacccact gatatcgaat
       61 cgtcactagg gccgtgatgg gagaagacat gcggagtatc tgtttccgga accgagcctc
      121 tgggttcgct gtttacgccg ctaagaacgt tggagattcc ggtcacggct aggctcatcg
      181 ctgcggtcgc tgccaacgta atgaatgcta ttgctcagaa tatccacaga atcgcttgcg
      241 cgcttctaga atcttgcggg cgcttatcgg aacatggcct gagactctgg tatcgctggc
      301 gcgcgccaag gctttttgct cgtcactgcc gggagaactt tgccatggtg ccgtcctgct
      361 tcaccatgcc gccactgaga taggtaaaaa taatgtcgct accgctgacc ctgtactccc
      421 acgtcattgg cgaatctccg tccacggtaa acgccacctt cggaccgtcc gtgaagtaag
      481 tcccgcccta catcgcagct tgcgtcgaga ccagtgccta cgttcctcgg acggtactgc
      541 aggccgcctt gccgtatatc caatggctgt tcatcgacat taagcacatg gattctggca
      601 agcacaagga aggcacgggt gtgccgaacg aactcattct cgacaacatt cgttggatca
      661 ggcgcacaaa ctggccggga cgcatggtgg tgcggatgcc cgttgttcct ggctttaacg
      721 ataccgtcgc caacgcggag gcaaccgctg agtttttgag agagataggt ctgagcgaga
      781 tcaacctgct gccgttccac cggttaggag cttcaaagta tgagcaactg ggtatgactt
      841 accaatatgt ggagcaggcc actgtggctc aagaaagtct tgagacactg gctatggtgt
      901 atcgtgaaaa ggctataacg tgccactccg gcgcggacac gccgttttag ctgcgcgcct
      961 cagggcgccc ataagagagg gtgcggacgg gacgtcggtg cccctggtct gatcctcgga
     1021 aaaaacccgc gtctgagaca ctgccgaaga tcaatatccg taccgctgtg cttcagtttg
     1081 ggaatctttg gacagaaaag gaacaaggtt cgcggttttc tggagggaca agttcaacga
     1141 attttcacac attaatcttg taatcgatgg tacttggctc caaccgcgac cgctctcttc
     1201 gcggactctt gcgaatcctg tccttcctga aaaatccatc cgactcctaa tagcagccca
     1261 cgcatcgaat ctcaagaccc gcatgtgttc ggattgtaga tcggaatctg cggaagaccg
     1321 cgcacagcac tcactgctcg cgtgcgagtg cggcaacgtg aaagcagacc acgtttggag
     1381 ccggttctat caggctgcgc ggcgttcgta tcggtggtgc agcgcgccga cttctggcaa
     1441 ctccacgact ctccggagtc tcgggcggct gaatagcacg agggatcggc gcgtccttgt
     1501 cgagtgacag atgcgttcgc caatgagcgt agtagtcaaa ttacgatctc agcactcgcc
     1561 tgagacccgg ctcgtgcaag acgattacat gatccagaca ttcgcggcgt atcgacccga
     1621 ccagtcgttc cgcataagcg ttctgccaag gtgaacgcgg agagcacaaa gcacttcctc
     1681 gatgccgagg ccggcgggcc gccgggggcc gccgccgcca agctgacgac aaggccgccg
     1741 ccactccaga ggccgcggca gaagagttgc ggaaggcggt gcagaacccg gcggccagcc
     1801 tgatcagcgt tcccctccag aacaacacca acttcgatat tgggccttat gaccgtaccc
     1861 agaacgtgct caacattcag cccgttatac cggtccacat caccaagaat tggaatctca
     1921 tcacccgcat tattcagccc atcgtatggc agccgtatcc ggaccagtcg accggaggcg
     1981 agcttggctt cggggacatg aatcccacgt tcttcctttc gcttttcaag ccgggcaagt
     2041 tgatctgggg tgctggcccg acagcgagcg gtctgcgtta tacacaattc cacacaacgg
     2101 cgcttctgct cccccacgcg tgcggcgctc ctcgctggtc gcaaccacca ttccagcgcc
     2161 accggcgtca tcatggaact cgccacgggt tacccgggct acaactcgct gatgcccaag
     2221 ggcaacggaa catttgccga agtgctcagg cagaacgggg ttcaagtgga gtcagaaaca
     2281 ggatgccgtg tcacgttgaa acgcagaatc ttgcgaccgt catggccgat tacgaagaag
     2341 cagtacagca ctccgaatgt gaagctgaca tatctgcacg ctgccgggcc tcaagtcatc
     2401 cattttgcag acgttcctaa ttttggtgaa ccacaggctg ccaaaccctc cttgctccgc
     2461 cacctaatct tctgctcgcc agcctttgtg cgccccggta ggccatcttt acaattgcgg
     2521 gccccgatgc tccgctcgga tcgaggtctc gtttgatgga gattcattcc ccgacgcaat
     2581 ttgcgac
//
LOCUS       DASRKK010000282         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_606922, whole genome shotgun sequence.
ACCESSION   DASRKK010000282 DASRKK010000000
VERSION     DASRKK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606922"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFC15_06195"
     CDS             complement(<1..938)
                     /locus_tag="VFC15_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM09787.1"
                     /translation="MNRWFAKLTCLLLLAAALPLAAQDLASYEKRVSVKTLPNGLTVV
                     LWRRPEAPVFSFFTLVDAGSAQDPRNETGLAHMMEHMAFKGTPDIGTTDYAAEKPALE
                     KVEQTYAVYEAERIKRVGQDPQKLAQLKTAWEQAIKDADKYVLQNQFGEIVESHGGVG
                     VNAFTNYDETGYMYSMPSNMIELWAALESDRLMHPVMREFYKERNVVMEERRMRTDSR
                     PTGRLVEQFLGTAFMANPYHRPTIGYASDLQSFSATDAAEFFKRHYVPSNMVIGLVGD
                     LDPAQVFPIVEKYFAQLPTTPKPVELFTDEPAQNSVR"
     gene            1446..2531
                     /gene="ligD"
                     /locus_tag="VFC15_06200"
     CDS             1446..2531
                     /gene="ligD"
                     /locus_tag="VFC15_06200"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZM09788.1"
                     /translation="MAKETVVDIEGKHLKLSNLDKVLYPEAGFTKGQVIDYYVRIAPV
                     LLPHLRGRPLTMKRYPNGVSGSFFYEKNCPEHRPEWVQTARVWSSGNNKWMNYCLAED
                     LPTLVWAANLADLELHTSLSLGKDILRPTFVVFDLDPGPPANIVQCCQVGLWVRDIFA
                     KLQLQSFAKTSGSKGLQIYVPLNTAITYDQTKPFAHELARLLERQHPDLVVSDMKKAL
                     RTGKVLVDWSQNDDHKTTVCVYSLRAKERPTVSTPVKWEEIENCLKKGDPTLLVFESD
                     EVLARAKKYGDLFEPVLKLMQKMPPLEALQRLEAGVTVKSEPATKRVVAIPEPKKKAA
                     APRTKSKASAAKSKASAAKKAPTRKRV"
BASE COUNT          547 a          740 c          782 g          517 t
ORIGIN      
        1 cgcaccgaat tctgcgccgg ctcgtcggtg aacaactcca ccggcttggg cgtggtggga
       61 agctgggcga aatatttttc cacgatggga aagacctgcg cgggatcgag atcgcccact
      121 aggccgatca ccatgttgct aggcacgtaa tgccgcttga agaactccgc cgcgtccgtg
      181 gcggagaagc tttgcagatc ggaagcgtaa ccgatggtcg gccgatggta aggattcgcc
      241 atgaaggccg tgcccagaaa ctgctcgacc agtcgtcccg tcggccggct gtcggtgcgc
      301 atgcggcgct cttccatcac cacgttgcgc tccttgtaga actcgcgcat caccgggtgc
      361 atcaaccggt cggactccag cgccgcccat agctcgatca tgttcgacgg catcgagtac
      421 atatagcccg tttcgtcgta gttggtgaag gcgttgacgc ccacgccgcc gtggctctcc
      481 acgatctcgc cgaattggtt ctgcaggacg tatttgtcgg cgtctttgat cgcttgctcc
      541 caggcggttt tcagttgcgc cagcttctgc ggatcctgtc ccacacgctt gatgcgctcg
      601 gcttcgtaca cagcgtatgt ctgctcaacc ttctccagcg cgggcttctc ggcggcatag
      661 tcggtggtgc caatgtcggg cgtgcccttg aaggccatgt gctccatcat gtgcgccagg
      721 ccggtctcgt tgcggggatc ctgcgcggag ccggcgtcca ccagggtgaa gaacgaaaac
      781 accggagctt cgggccgtcg ccagaggacg accgtcaagc cgttgggcag ggtcttcacc
      841 gagactcgct tttcgtacga cgccaggtcc tgtgccgcta gcggcaaggc ggccgccagc
      901 aggagcaggc aagttaattt ggcaaaccag cggttcatcg tgcctccaga agggcgtgac
      961 tatgcacaag ttagaaagtt acagataagg acgcgcaagt caaacggagg cgagcaggag
     1021 agcaaccgca tgtcttagtt catcgtcctg agaaatgatt cgagaggccg tgccttgaga
     1081 tggctgttca tcagcgcacg taattttgaa gtgtcatccc gagcaacgag cgtagcgagg
     1141 agtcgaggga cccctttggc cgtcaacaat cccaactttg ttcctgtccc aggcggccac
     1201 actgtgattc ttgcagttat aggggtcctt cgactcgctg cgctcgctca ggatgacaga
     1261 gagaaaaacg gggatcctcc gacttcgcgc ccgcgcgacc ctcaaaagcg ggtcgaaaat
     1321 ttttgcggcc gctccgctca tcaggatgac actttcaaac taatgaataa tccaacttcg
     1381 tgcccgcagt tgcatacttc ctcgctcaac ctgcatcatt tctgtgcagc gcgcgcgagg
     1441 caacgatggc caaagagacg gtggttgaca tcgagggaaa gcacctgaag ctttcgaacc
     1501 tcgacaaggt tctgtatccc gaagccggat tcaccaaagg gcaggtgatc gactattacg
     1561 tgcgcatcgc gccagtgctg ctgcctcacc tgcgcggccg gccgttgacc atgaagcgct
     1621 atcccaatgg cgtgagcgga tcgtttttct acgagaagaa ctgtcccgag catcgtcccg
     1681 agtgggtgca aaccgcgcgc gtctggagtt ccggcaataa caagtggatg aactactgcc
     1741 tggccgagga cctgccgacg ctggtgtggg cggccaacct cgctgatctg gaactgcaca
     1801 ctagcttgtc gctaggcaag gacattctgc ggccgacgtt cgtggtcttc gatctcgatc
     1861 ctgggccgcc cgccaacatt gtgcaatgtt gtcaggtcgg cctgtgggtg cgcgacatct
     1921 tcgccaaact gcagttgcaa agtttcgcaa aaacttccgg gtcgaagggc ctgcagattt
     1981 acgtcccgct gaacaccgcc attacttacg accagaccaa acccttcgcg catgagctgg
     2041 cacgattatt ggagcggcag catccagacc tggtcgtctc ggacatgaag aaggctctgc
     2101 gcaccggcaa ggtgctggtg gattggagcc agaacgacga tcacaagacg acggtttgtg
     2161 tgtactcgtt gcgggcgaag gagcggccga cggtttccac gccggtgaag tgggaggaaa
     2221 ttgagaattg cctcaagaaa ggcgatccaa ctttgctggt gttcgaatcg gatgaagtgt
     2281 tggcgcgagc caagaagtat ggcgacctgt tcgagccagt gttgaagctg atgcagaaga
     2341 tgccgccgct ggaggcgctg cagcggttgg aagcgggcgt gactgtgaag tccgagcctg
     2401 cgacgaagcg cgtagtggcg attccggagc ccaagaagaa ggcagccgcc ccgcgcacga
     2461 aaagcaaggc cagcgctgcg aagagcaaag ccagcgctgc caagaaggca cctacgcgca
     2521 agcgcgtgtg atattgtttc cagcgcaatg agtccagcca gcaagaaaaa gaacgatgct
     2581 ccgccc
//
LOCUS       DASRKK010000283         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_802861, whole genome shotgun sequence.
ACCESSION   DASRKK010000283 DASRKK010000000
VERSION     DASRKK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802861"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFC15_06205"
     CDS             <1..1177
                     /locus_tag="VFC15_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09789.1"
                     /translation="YELREHTSAKEKLYITCHYHDLVTGDIDQTIAAYRLLRQTYPHE
                     ESASTNLNSAYTSIGRYEQALVEAQSALQVDPSDVINYTNLAGTYLNLNRFNDMKAVL
                     EQAQARHLENQAMIWDFYAMAFLRGDSEEMARQLAAAMGKPGMEDQMLATQADTDAYF
                     GRLGKARESTRLAQAAAMRAGSQEAAALWQLDGALHEAELGDPQRARQQGLEAMAAGS
                     SKNAQIVAALVLARSGDRKRAQSLADALSRQYASDTLVNNYWLPSIRAAIALDEKNPG
                     AALEALQPAAPYELGSPFPGIAFLYPVYLRGMAYFQAAEGKLAAAEFQKILDHRGIIL
                     NFPTAALAHLQLARAKAMSGDKDGAREAYRDFLTLWKGADADNPVLRQAKAEYEKLK"
     gene            1624..2511
                     /locus_tag="VFC15_06210"
     CDS             1624..2511
                     /locus_tag="VFC15_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM09790.1"
                     /translation="MPDDINEEQKPPEAHPAPTDQSDAQRAQRVPEDAATQEEVAAPA
                     VLRTTGPAGEPVRIVTPAERRRMNRRELLKLVPVLALGAFAVPKLREPLLNEGLHLSD
                     WASEKLFGRQRLAQTFRDSDVVAFERFPYNFYDVADPGVDLERWTLTVEGLVKRPGEY
                     KLSQIQAFQKVTQNTRHVCVEGWDVIGNFGGTRASDFLRSIGADTSARFLEAECVDGY
                     YESIDMATVLHPQTLFCYEMYGKPLDRGHGAPLRLQMPTKLGYKQAKYLDTIRVTNVL
                     RADKRGYWEDQGYDWYGGL"
     gene            2524..>2862
                     /locus_tag="VFC15_06215"
     CDS             2524..>2862
                     /locus_tag="VFC15_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZM09791.1"
                     /translation="MATIANVLKQNVIDSPPVTIAYEHPWVVRFAHWVNAVALLVLVG
                     TGLQIFRAFPSFGPKIPQKNLIDVPKLITLGGWLGGALQWHFTFMWIFVASGLMYLIY
                     QLATGHWRQVL"
BASE COUNT          618 a          831 c          840 g          573 t
ORIGIN      
        1 ctatgaactg cgcgagcaca ccagcgcgaa agagaagttg tacattacct gccactatca
       61 cgatctggtt actggcgaca tcgaccagac catcgccgcc taccggctgc tgcgccagac
      121 ctatcctcat gaagagtctg cttccaccaa tttgaattct gcctacacct ctatcggaag
      181 gtacgaacag gccctggtgg aagcgcagag cgcattgcag gtggatccca gcgacgttat
      241 caactacacg aacctcgcgg gaacctatct caacctgaac cgcttcaatg acatgaaggc
      301 ggtattggaa caagcgcagg cccgccatct ggagaaccag gcgatgatct gggacttcta
      361 cgcgatggct tttctgcgcg gcgattcaga ggaaatggcg cgccagttgg cagcggccat
      421 gggcaaaccg gggatggaag accagatgct cgcaacgcaa gctgacaccg acgcctattt
      481 tggccgtctg gggaaggcac gcgagtccac gcgtttggca caggcggcgg cgatgcgcgc
      541 gggctctcag gaggctgcgg cgttgtggca gttggacgga gccctccacg aagccgaatt
      601 gggcgatcct cagcgcgcgc ggcagcaggg tttggaagcc atggccgccg gatcgagcaa
      661 gaacgcgcaa atcgtggcag cccttgtgct ggcacgtagc ggtgatcgca aacgggcgca
      721 gtcgctggct gatgccctca gcagacaata tgcctcggac actttagtca acaattactg
      781 gctgccctcc atacgagcag cgattgccct ggacgagaaa aatccaggcg ccgccctgga
      841 ggctttgcaa ccggcagcac cttatgaact gggttcgcct tttccgggaa tagcttttct
      901 ttaccctgtt tatttgcggg gaatggccta tttccaggcg gccgaaggca agctggcggc
      961 tgccgagttc caaaaaatcc tcgaccatcg cggcatcata ctgaacttcc ccaccgccgc
     1021 cctggcgcat ctccagttgg cccgagccaa agcgatgagc ggagacaagg acggagcccg
     1081 cgaagcctat cgcgacttcc tgacattgtg gaagggcgcc gatgccgaca atcccgtgct
     1141 gcggcaagcc aaagcggagt acgaaaaact gaagtgaact cctccagcat agcctggagc
     1201 gatttcaacg ttaggtggtg tatccggctt atcggcagag cagtcctaag aagattgtgt
     1261 cgtgaccttg tcgcttcgtc acagtggaag gaagtacaca agaaccagcc ccgaatgggg
     1321 cgaaagagct tagcccagcg cttcagcgct gggtatagtg ggaaataaga ccgagtcccg
     1381 tagggacggc aaagttttca cgcattctct tcagcccgcg aaggatccgt ttttccgact
     1441 ttttcagcag cctgcacagc aggaagctgg ctctatcgca atgaatttcg gagtgcgcac
     1501 ggtaatccgc ccggcgggac acctagggac acctgccttc tcttgcattg gagcgcttct
     1561 ccggtgtaac ttttgcctcg ctcgcccatc tgattagcaa gcctgttcgt atcgaggtaa
     1621 gacatgcccg acgacatcaa cgaagaacag aagccgcctg aggcacaccc ggcaccgacc
     1681 gatcagagcg atgcccaacg ggcacagcgg gttccagaag acgccgcgac ccaagaagaa
     1741 gtcgccgccc ctgccgtgct gcgcaccacc ggtccggcgg gcgagccagt gcggatcgtc
     1801 actcctgccg agcgcagacg catgaatcgt cgcgaactgc tgaagctggt tccggtgctg
     1861 gcgctgggag cgtttgccgt acccaagctt cgtgaacctc tcttgaacga ggggcttcac
     1921 ctgagcgact gggcctctga aaagctcttc ggccgccagc ggctggcgca gacctttcgc
     1981 gatagcgatg tagttgcctt cgaacgattt ccttacaact tctatgacgt cgccgatcca
     2041 ggggtggacc tggagaggtg gacgctgacg gtggagggcc tggtcaaacg tcctggcgaa
     2101 tacaagcttt cccagattca ggcatttcag aaggtcacgc agaacactcg gcacgtgtgc
     2161 gtggaaggtt gggacgtgat cggaaatttc ggtggcacgc gggcctccga ttttctgcga
     2221 tcgatcggcg ccgataccag cgcccgcttt ctcgaagccg aatgcgtgga tggatattac
     2281 gaatcgatcg atatggcgac ggtgctccat ccgcagacat tgttttgtta cgaaatgtat
     2341 ggcaagccgc tcgaccgcgg acatggcgcc ccgctccgtc tgcaaatgcc taccaaactc
     2401 ggctataagc aggcgaaata tctcgacact atccgagtga ccaacgtgct gagagctgac
     2461 aagcgcggct actgggaaga ccagggctat gattggtacg gcggcctctg aggaaaagtg
     2521 ctgatggcaa cgattgcgaa tgtgctaaaa caaaacgtga tcgactctcc tcccgtgacc
     2581 attgcctatg agcacccctg ggtggtgcgt ttcgcccact gggtgaacgc ggtggctttg
     2641 ctggtcctgg tgggcaccgg tctccagatc ttccgcgcct ttcccagctt cggtcccaag
     2701 attcctcaga aaaacttgat tgatgttcct aagctgatca cgctcggcgg atggctcggc
     2761 ggcgccttgc agtggcactt caccttcatg tggatcttcg ttgccagcgg gctgatgtac
     2821 ttgatctacc agcttgccac cggccattgg cggcaggtgc tg
//
LOCUS       DASRKK010000284         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_21091, whole genome shotgun sequence.
ACCESSION   DASRKK010000284 DASRKK010000000
VERSION     DASRKK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21091"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VFC15_06220"
     CDS             complement(<1..1266)
                     /locus_tag="VFC15_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09792.1"
                     /translation="MRSLLLNLSLLWVLLIPAFAQQPDSSQSQPKAEPQTKTNAPSKK
                     AQSQPAKSPETPTATEEPKPEPTKESGDKAKDKEEHFDMTEVAPVVTHHQIGVNGKLL
                     HYTATTGRLPIKRADGKIEAEMFFVAYTLDGQEASKRPLTFAFNGGPGSSSIWLHMGA
                     LGPKRVVLQPDGFMPPAPYRLEDNPYTLLDKSDLVLVDAIGTGFSRAGDAELFKKFWG
                     VQGDIEAFSEFIRLYITRNERWSSPLYLFGESYGTTRSAGIAGYLADKGISFNGITLL
                     SMVLNFETLISNKTNDQAYVFLVPSFTMIAGYHHKLAPDLAQDMNKAREESQHWASTD
                     YALALDKGDALTPEERQKVITQLARYTGLSADVIDQANLRIDVRKFTHYLLIDQKVRV
                     GRLDGRFTGPDPNGLLDTPFYDPSGSAPTP"
     gene            complement(1588..2463)
                     /locus_tag="VFC15_06225"
     CDS             complement(1588..2463)
                     /locus_tag="VFC15_06225"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HZM09793.1"
                     /translation="MPKQSHSSRPLTVSIVGPGNLGTALALTLPSAGYVVKFIAARAK
                     GITSRETTGLARRVNARLVTLGKKPLDSDLVWITVPDDAIAATTLRLARTQDWQGKIV
                     FHSSGALTSDELAPLREQGAKVASVHPGMTFVRGPAPQMLGVAFAVEGDPAATRVAKR
                     IVADFGGTTYAINKRNKVLYHAFGSFASPLVIALMASLERVAKAAGIKPDDVKAMMVP
                     LLRQTLRNYLQHDAASAFSGPLARGDVATVCRHLEELKAVPGARAVYVALASAALKNL
                     PVKNRRAMERELKRG"
     gene            2813..>3373
                     /locus_tag="VFC15_06230"
     CDS             2813..>3373
                     /locus_tag="VFC15_06230"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZM09794.1"
                     /translation="MPSKKLVLGKPDSGRKSKKPSRPAKKVEVLSSKTVFKGPAFSVN
                     CDRVREPSGIVARRDVVRHSGSAVILAVDDSGPEPRVLLERQYRYAAGNYLWELPAGR
                     VDRGEKALAGAKRELLEETGYRAAKWKQALFFYPSPGFLDETMTVYLARGLKPGDAQP
                     EEDEEIECHLTPLSQALAMVRSGEICD"
BASE COUNT          677 a          988 c          983 g          725 t
ORIGIN      
        1 cggggtaggg gccgatcccg atggatcata gaacggcgta tccagcagtc cattggggtc
       61 ggggccggtg aaacggccat ccaggcggcc tacgcgcacc ttctggtcga tcagcaggta
      121 gtgagtgaac ttgcgcacat caatacgcag atttgcctga tcgatcacat cagcgcttaa
      181 acctgtatag cgtgccaact gggtgatcac cttctgccgc tcctccggcg tcagggcgtc
      241 tcccttatcg agcgccaggg catagtcggt cgatgcccag tgctgagatt cttcgcgtgc
      301 cttgttcatg tcctgggcaa gatcgggcgc cagcttgtga tggtatccgg cgatcatggt
      361 gaacgagggg accaggaaaa cataagcctg gtcgttggtc ttgttgctga tcagggtctc
      421 gaaattcagc accatcgaaa gcagcgtgat gccattgaag gagatgccct tgtcggcaag
      481 gtatcctgcg atacccgctg agcgcgtcgt gccatagctt tcaccgaaca ggtagagggg
      541 tgaactccag cgttcatttc gcgtgatgta gagacgaatg aattcgctga atgcttcgat
      601 gtcaccctgc actccccaga atttcttgaa tagctccgcg tcgcccgcac gactgaaccc
      661 tgtgcctatc gcatccacca gcaccaggtc gctcttgtcc agcagcgtgt aggggttgtc
      721 ttcgaggcgg tagggcgcgg gcggcatgaa cccgtcaggc tgcaagacca cccgcttggg
      781 gccaagcgcc cccatgtgca gccaaatcga cgacgaaccc ggcccgccgt tgaaggcgaa
      841 tgtcagggga cgcttgctgg cttcctgtcc atccagcgtg taagcaacga aaaacatctc
      901 ggcttcgatc ttgccgtcag cgcgcttaat cggaaggcgt ccggtggtgg ccgtgtagtg
      961 cagcagtttc ccattcaccc cgatctggtg atgggtcacc accggagcca cttcagtcat
     1021 gtcgaagtgt tcttctttgt ccttggcctt gtcgccggat tccttagtcg gttccggctt
     1081 tggttcttca gtcgccgtcg gggtttctgg ggatttcgcc ggctgtgact gcgctttctt
     1141 cgatggtgcg ttcgtctttg tttgcggttc tgccttgggc tggctctggg acgaatctgg
     1201 ctgttgcgca aacgctggaa tcagcaatac ccataacaaa gataggttca aaagaagaga
     1261 acgcattcga tctcctgcaa actatcggat tgcgacattc taaaccgctt acagaattgg
     1321 tatatccgtc agcacgggaa gtgagatctg ctttcccgac tttttcagca gccccttcag
     1381 ccgtgccgta actactgtca tttatgatgg cttagccact gagggatctc tgggttcaaa
     1441 cgaagtgcga ctcccctcag gggctgaagc ccaactctcc acgcacacct tacggcacgg
     1501 ctgaagccgt gccctttcaa agcttgctgc gtgagttacc gaaacagatg aataatccaa
     1561 gttcagtgct gcgttcaccg cgaaccccta ccctcttttc aactcccgct ccatcgccct
     1621 gcggttcttg accggtaggt tcttcaacgc cgcgctagcc aacgcgacgt acaccgcgcg
     1681 tgcccccgga actgccttca actcttcaag gtggcggcag accgtagcga catctccgcg
     1741 tgccagcgga ccgctgaaag cggaagcggc gtcgtgctgc aagtaatttc gcagggtctg
     1801 cctcagtagc ggaaccatca tcgccttgac gtcgtcaggc ttgatcccag cggccttcgc
     1861 cacgcgctcc agcgaggcca tcagggcgat caccaagggc gaggcaaaac tgccgaaggc
     1921 gtgatacagc accttgttgc gcttattgat cgcatacgtc gtgccgccga agtctgccac
     1981 gattcgctta gccacgcgag tggcggccgg atcgccctcg acagcaaatg ccacccccaa
     2041 catctggggc gcggggcctc gcacaaaggt catgcccgga tgcaccgacg ccaccttggc
     2101 tccttgctca cgcagcggag ccagttcgtc gctggtcagg gcgccgctgg agtggaagac
     2161 gatcttcccc tgccagtcct gggttcgcgc cagtcgcagt gtagttgcgg caatcgcgtc
     2221 atcgggtacg gtgatccaga cgagatcgct gtcgagcggc ttcttgccca gcgtaaccag
     2281 tcgcgcattc actcttcgcg ccaggcctgt cgtttcgcgg ctggtgatgc ccttggcgcg
     2341 cgcagcaatg aacttcacca cgtatcccgc ggatggcaaa gtcaacgcta aagccgtccc
     2401 taaattccct ggaccgacga tgctgaccgt caggggacga gaggagtgag attgtttggg
     2461 cacgccgggc agaatagcag atttcgcgcg gttcaacagg ctgcgggaaa actctgtgga
     2521 agacttttgg tggaaacccc ctgctccagc agggggagat ggacttcagt ccagcggaaa
     2581 agcgttcgat atataagatg ggcttttagc cttgggactt ctcgatcccg gcgctaaagc
     2641 gcaagattga agtcagactt ttcccggagc gctgaagcgc tccttccccc gcataaatgc
     2701 gggggctccc accgagaagt acactccaca ggttgttgca cagacttttt ccgcagcctg
     2761 tcaatgcggc cgttacgaaa actcgctaca actgccttat agtggtgaga ctatgccatc
     2821 aaaaaagctc gtgttgggaa agccagacag cggccgcaaa tcgaaaaagc cgtcccgtcc
     2881 ggcgaaaaaa gttgaggtcc tctcgtcgaa aaccgtattc aaaggacctg cattcagcgt
     2941 gaattgcgac cgcgtgcgcg aaccgagcgg aattgttgcg cgccgtgacg tggtacggca
     3001 ctccgggtcg gccgtaattc ttgcggtgga cgatagcggc cccgaaccgc gcgttctgtt
     3061 ggagcgccaa taccgttacg ccgctggtaa ctatctttgg gaactgcctg ctggccgcgt
     3121 cgatcgtggg gaaaaggccc tggccggtgc gaagcgcgaa ctgctggagg aaaccggata
     3181 tcgcgcggcc aagtggaagc aagctctgtt cttctatccg agcccgggtt tcctggacga
     3241 gaccatgacg gtttacctcg cccgtgggct gaagcccggc gatgcgcagc ccgaggagga
     3301 cgaagaaatc gagtgccacc tgacgccttt gtcccaggcg ttggcgatgg tccggtcggg
     3361 cgagatttgc gat
//
LOCUS       DASRKK010000285         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_118220, whole genome shotgun sequence.
ACCESSION   DASRKK010000285 DASRKK010000000
VERSION     DASRKK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118220"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..916)
                     /locus_tag="VFC15_06235"
     CDS             complement(89..916)
                     /locus_tag="VFC15_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09795.1"
                     /translation="MNQGSNPPSGGQPGGKGRRRRRGRRGPVAQGAAPQQGRGPGQGQ
                     GSAHGQNQGPGQGPGRNQSARRFGKKRPPGRKPGSRPAQREPIGGGIYSAPMDHSYRA
                     AQGNNDGNTHNPTQGRNTQSFGKQFSVPEPEPLAVISDAPARIYAFVDDLFFLAKIQE
                     TARKMNIKVEFVKTDKDVYERMGQNGNDKPTLIIVDLNNVAAKPLTVIPKLKSKLKKE
                     TNIIGFVSHVQGELKMKATEAGCDMVLPRSAFSQNLAQLLRRHAVQEEELPEHNQPS"
     gene            complement(1397..2254)
                     /locus_tag="VFC15_06240"
     CDS             complement(1397..2254)
                     /locus_tag="VFC15_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZM09796.1"
                     /translation="MAEADLYRSALRGFTALVEPSHEDASIDLAVAALAIARTEYPKL
                     DVSYYVGRLDALARRVRGRMRSDPTAREVIALLNRVLFDEEGLRGNRDDYYDPRNSFL
                     NDVLDRKLGIPITLSVIYMDVARRVGFPIAGTGMPGHFLLKHYDPMAGEIIIDPFNRG
                     RIVGNAECQQRLNEIYSGEIELQPEHLRPVTHREILTRILNNLRQIYFTQRNFPKGLT
                     VLDLLLAIPPRSADLLRERGLVRLNLEQFLGAAQDLGAYLQLQPEAPDADDVRETLDM
                     LRQYLARLN"
     gene            complement(2308..3228)
                     /locus_tag="VFC15_06245"
     CDS             complement(2308..3228)
                     /locus_tag="VFC15_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZM09797.1"
                     /translation="MLRTFFIKLSENQSIRHFAENSALGLRFSSRFVAGTQLADVIRV
                     TEKVNQTGASVSIDNLGENVTNTDEAKHSAELYHHMLDEISTRKLNANISLKLTHMGF
                     DVDEDLAFRQVTELVSHAVQINSFVRVDMEGSPYTQRTLDFVRQLHRQPGHAGKVGTV
                     IQTYLNRSEDDVNQLLSERIRIRLCKGAYKEPPEIAFPKKADVDANYLKLAKTLLKSG
                     VYHGLATHDEKIIEEMKQFARAEDIPSTAYEFQMLHGVRRDLQQKLVREGYGLRVYIP
                     FGTEWYPYFMRRLAERPANVFFIARNMFRQ"
BASE COUNT          735 a         1026 c         1121 g          831 t
ORIGIN      
        1 tccagcctgg caattcaaat cgaggactca cacccggtct cagggtccga acaactgcgc
       61 acgcaggtac attgattctg gaacctctct agctcggctg attgtgttcc ggcaactctt
      121 cttcttgaac cgcgtggcgc cgcaacagtt gcgcgaggtt ctgggaaaat gcggaacgcg
      181 gcaggaccat gtcgcagccg gcttccgtgg ccttcatttt cagttcgccc tgcacgtgag
      241 acacaaagcc gatgatgttg gtttccttct tcaacttgct cttgagttta gggatcacgg
      301 tgaggggctt ggcggcgaca ttattcaggt caacgataat caacgtgggc ttgtcgttgc
      361 cgttttgtcc catgcgctca taaacgtctt tgtcggtctt gacgaattcg accttgatgt
      421 tcatcttgcg cgccgtttcc tggatcttgg cgaggaagaa caggtcgtcg acaaacgcat
      481 agatccgcgc gggtgcgtcg ctgattacgg ccagcggctc aggctcggga actgagaact
      541 gcttgccgaa agattgggta ttgcgacctt gggttggatt gtgcgtgttg ccgtcgttgt
      601 tgccttgcgc agcgcgataa ctgtggtcca tgggggcgga gtaaatgcca ccgccgatgg
      661 gttcgcgctg ggctggacgg ctacccggct tgcgtccggg aggacgtttc ttgccgaaac
      721 ggcgggcact ctggttgcgg cccgggcctt gacccggtcc ctgattttgc ccgtgggcgg
      781 aaccctgtcc ctggccgggg cctcgtccct gttgtggggc tgcgccttgc gcaaccggtc
      841 cgcggcggcc gcgccgccgt ctacgtccct tccctccggg ctgtccgccg gaaggaggat
      901 tgcttccttg attcatgttg ctctcttagt tacgttcgtt ttcccgcggg tcgcgggact
      961 cccacatttg caaaccgcgc ttgctggccg tgtgttcggg tcctagcccg caaaatcggc
     1021 aagtctgcga ttggaatcgt ctatgaaatc gaatcagtta cgtccgtctg gcgctcggaa
     1081 cccacggcgc gaagaacgcc agcacgggaa gtgcggcgat tggaggacgt acctatggta
     1141 cccagctttg ctatcgaaac gcaagcggca aagcctgtta ttaatttgat tactcacttt
     1201 cgccagtccg ggaacatcaa caccggacga atttgaaaat cgcaaacaca aaccgacgcg
     1261 gggaatcggc tcgcgattat tgttcaattg ggggccggag taggcgaagg atctcgccgc
     1321 ttcgcacaga gtgcatcaat ttccggaatt gtaccaacaa agtccagcga atggcgaatc
     1381 gtggcaccga gctgcgtcaa ttcagccttg ccaggtattg tcgcaacatg tcgagggtct
     1441 ctcgcacatc gtccgcgtca ggggcctccg gctgcaattg caggtaggcg cctaaatcct
     1501 gagcagcacc cagaaactgc tccaggttga ggcgcaccaa accgcgttcg cgcaacaagt
     1561 cggcgctgcg cgggggaatc gccaacagca aatcgaggac tgtcaagccc ttgggaaaat
     1621 tgcgctgcgt gaaataaatc tgccgcagat tattcaagat gcgggtcagg atttcgcgat
     1681 gggtcaccgg tcgcaggtgc tccggctgca actcgatctc accggaataa atctcattca
     1741 ggcgctgctg gcactcggcg ttgcccacga ttctcccgcg attaaaggga tcgatgataa
     1801 tctcccctgc catcggatcg tagtgcttga gcaggaagtg tccgggcatg ccggtgccgg
     1861 cgatgggaaa gcccacgcgg cgagccacgt ccatgtaaat caccgacagg gtgatgggga
     1921 tgccgagttt gcggtcgagc acgtcgttaa ggaacgaatt gcgaggatcg tagtagtcat
     1981 cgcgattgcc gcgcagtccc tcttcgtcga acagcacgcg gttgagcagc gcaatcacct
     2041 cgcgagcggt gggatcgctg cgcatgcgtc cgcgaacccg acgcgccagg gcatcgaggc
     2101 gtcccacata gtaggacacg tcaagcttgg gatactcggt gcgcgcaatc gccagggccg
     2161 cgaccgccag atcgatggac gcatcctcgt gcgagggttc gaccagggca gtaaagcccc
     2221 gcagggcgct tcggtataaa tcggcttctg ccatcgcgtg ccgattggat gaaccgcacg
     2281 gcaaacgcga ttcgcgtcgt aaggcgttta ctggcgaaac atattcctgg caatgaagaa
     2341 cacattcgcg gggcgctcgg ccagtcgccg catgaaatac ggataccact ccgtgccgaa
     2401 cggaatgtac accctcaggc catagccttc gcgcaccagc ttctgctgca agtcgcgacg
     2461 caccccgtgc agcatctgga attcatacgc cgtcgacgga atgtcttcgg cgcgagcgaa
     2521 ctgtttcatc tcctcgatga tcttttcgtc gtgcgtggcc agcccgtgat acacgccgct
     2581 tttcagcaga gtcttcgcca gcttgaggta gttggcgtcc acgtcggcct tcttggggaa
     2641 ggcgatctcc ggcggctcct tgtaggctcc cttgcacagc ctgatgcgga tgcgttccga
     2701 gagcagctga ttcacatcgt cttcgctgcg attcaggtac gtctggatca cggtgcccac
     2761 cttgcccgcg tgtcccggtt ggcgatgcag ttggcggacg aagtcgaggg tgcgctgggt
     2821 gtagggcgag ccttccatat cgacgcgcac aaagctgttg atctgcaccg cgtgactcac
     2881 cagctcggta acctggcgga aggcaaggtc ttcgtccacg tcgaagccca tgtgggtcag
     2941 ctttaggctg atattggcgt tcagtttgcg cgtcgagatc tcgtcgagca tgtggtgata
     3001 gagctcggcg ctgtgtttgg cttcgtcggt attggtgacg ttctcgccga ggttgtcgat
     3061 gctgacactg gcaccggttt gattgacctt ctcggtaacg cggatgacgt cggcaagttg
     3121 ggttccggcg acgaagcggc tggagaagcg cagcccaagg gccgagtttt cggcgaaatg
     3181 gcgaatggat tggttctcgg aaagcttgat gaagaaggtt ctcagcatgc gacctccgcg
     3241 ggcccacaag gcagaggctc ggcattagaa gggataagag tatggcgcat aagaatgttg
     3301 gggactgcta accaaacctt cgatgatagc gctcgggcgg gtgtggggac aaactgctcg
     3361 cggatcgggg tagtgtctga aaactgcgtt gatgattaac agcgagcgct aagcagtccg
     3421 gttccagccg ggctgtttca cgtttacgcg gatttccccc cgccaatata atcgaattgg
     3481 tgaactaagc atggaaacaa tgtgttacga ggcacattcc ccgaattcag acgtgccaat
     3541 ctgcggattg catcaaaagc cgctgattga aaagagctta ccgaccgccg atcagaatcc
     3601 tcagggctta ggtaaaggtg tattgcgata ccaccttact gtgcttgttt gcccagtaac
     3661 taaggaaact gtaaggcggc tgtgattgct ctgctcgcct ttgcctttgt ttt
//
LOCUS       DASRKK010000286         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_21289, whole genome shotgun sequence.
ACCESSION   DASRKK010000286 DASRKK010000000
VERSION     DASRKK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21289"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2527
                     /locus_tag="VFC15_06250"
     CDS             <1..2527
                     /locus_tag="VFC15_06250"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM09798.1"
                     /translation="TTGAGKYTDDLSLPGMLVGKILHSPYPHARIKRIDTSRAKDLDG
                     VVAVVTGADAPNPYGILPVGHDETALAVEKVRYVGDHVACVAAISEEIAENAIELIDV
                     EYELLPAYFDPVESMKAETDLIHENRAHNIEKDYHHVFGDLDKGFAEADYIAEGRYLA
                     NEVTHAAMEPHSTLANFELDPHTGKMGRLTVWSSTQVPYYLQHKLSLVLEMPMSQIRV
                     IKPLVGGGFGGKSEIIPLEIIAAVAARAARAPVKITYTREEVFWAHRGRPRTIIDLKT
                     GVKNDGRMTAVACKVIQDGGAYCSYGVVTILYSGALLGALYDIPNIRYDGYRVLTNKP
                     ACGAMRGHGTVNVRFAFESQMDEIAAKLKLDPADMRRVNLLKPPTVTVNGLRVLSYGL
                     PECIDQVVTRSGWKERKGKLPKGRGLGVACSHYVSGAANSIIRSDMPHSTVNIKIDRD
                     GGVVVYTGASEIGQGSDTMTAQVVAEVLGCEISRVKVIAADTDLTPIDLGSYSSRVTF
                     MAGNAALRAAEDVKKNIVSAAAKRMDCVPEDVIMRNDRVWNGGTTLSDYDFTASDSLE
                     DKEAARGHVDGQILRGSVLQTQKEDGPKDHMSFEEAIVAAIDFHGALTGTGSYAPPVA
                     ARGGKHKGGGVGPSPAYSYSAQVAEVSVDEDTGEVTVHKVWAAHDCGRALNPVAVEGQ
                     VIGSVWMGLGQALEEEMVWKDGLLMNPGLLEYRSPSSTESPEIECIIVESIDPEGPFG
                     AKEAGEGSLAATIPAISNAIYDAVGVRLREAPFTPERVLAALRAKKGMKRIEMTESVD
                     PTAPDKFREHGGSLWFRGKGPMRHPLDPARQGTNAGSPAGGGD"
BASE COUNT          545 a          727 c          851 g          467 t
ORIGIN      
        1 aaccaccggc gcgggcaagt acacggacga cctcagcctt cctgggatgc tggtcggcaa
       61 gattctccat tcaccctacc cacacgctcg aatcaagcgt atcgatactt cgcgagcgaa
      121 ggacctggat ggagtcgtcg ctgtcgtgac cggggcggac gcgccgaatc catacggcat
      181 tcttccggtg ggccacgatg agaccgcgct ggcggtggag aaggtccggt acgtaggcga
      241 tcatgttgcc tgcgttgcag cgatcagcga ggagattgcg gagaacgcga tcgagttgat
      301 cgacgtggaa tacgagcttc tgcccgccta cttcgatccc gtcgaatcga tgaaggcgga
      361 gacggacctc attcacgaga accgcgcgca caatatcgag aaggactacc accatgtctt
      421 cggcgatctg gacaagggat ttgccgaagc cgactacatc gccgagggac ggtacctcgc
      481 caacgaagtg acgcacgctg cgatggagcc gcacagcacg ctggcgaact tcgaactcga
      541 tccccacacc ggcaagatgg gacggcttac ggtgtggtcg tccacgcagg ttccttacta
      601 tctgcaacac aagctttcgc tggtgctgga gatgccgatg tcgcaaatcc gcgtgatcaa
      661 acccttggta ggcggcggct ttggcggcaa gagcgaaatc atcccgctgg agatcatcgc
      721 ggcggtggcg gcgcgcgcag cgcgagcccc agtgaagatc acttacacgc gcgaggaggt
      781 tttctgggcg catcgcgggc gcccgcgcac tatcatcgat cttaagacgg gcgtgaagaa
      841 cgatggacgc atgaccgctg tggcctgcaa ggtcatccag gacggtggcg cctactgctc
      901 gtatggcgtg gtgacgattc tctattccgg tgcgctgcta ggcgcgctgt acgacatccc
      961 caacatccga tatgacggct atcgcgtgct taccaacaag cccgcttgcg gagctatgcg
     1021 aggccacggc acagtcaatg tgcgtttcgc cttcgagtca cagatggacg agatcgccgc
     1081 gaaactcaaa cttgatccgg cggacatgcg acgcgtcaac ctgttgaagc cgccgaccgt
     1141 aaccgtgaac ggtttacggg tgctgagcta cgggttgccg gagtgcatcg atcaggtggt
     1201 tactcgatcg ggttggaagg agcgcaaagg caaactgccc aagggccgcg gccttggagt
     1261 ggcgtgcagc cactatgtga gcggcgcggc caacagcatt atccgctccg acatgccgca
     1321 ttcgacggtg aacatcaaaa tcgatcgcga tggtggcgtg gtagtttaca ccggagcttc
     1381 ggagatcggg caaggctccg atactatgac cgcgcaagtt gtggcggagg tgctgggttg
     1441 tgaaatctca cgcgtcaaag tcattgccgc cgataccgat ctgaccccga tcgatctcgg
     1501 ttcctactca agccgcgtga cctttatggc tggcaatgcc gctctgcgcg ccgccgaaga
     1561 tgtgaagaag aacatcgtaa gtgccgccgc caagcggatg gattgcgtcc ccgaagacgt
     1621 gatcatgcgc aacgaccggg tgtggaacgg aggtactacg ctgtcagact atgacttcac
     1681 cgcgtcggac agcctggaag acaaagaagc tgcacgtgga catgtggacg gccagatcct
     1741 tcgtggatcg gtgttgcaga cgcaaaaaga ggatggcccg aaggaccata tgagcttcga
     1801 ggaggctatt gtcgcggcga ttgatttcca cggtgcgctg accggcactg gctcgtacgc
     1861 accacctgtg gcggcacgcg gcggcaaaca caaaggcggc ggagtggggc cgtcgccagc
     1921 gtattcgtat tccgcacagg ttgccgaggt cagcgtggat gaagacaccg gcgaagtgac
     1981 ggtgcacaag gtatgggccg cccacgactg cggacgcgcg ctgaacccgg tggccgtcga
     2041 aggccaggtg atcggctcgg tctggatggg acttggccag gcgcttgagg aagagatggt
     2101 ctggaaagac gggctgctaa tgaatcccgg gctgctggaa tacaggagtc cgtcgtcaac
     2161 tgagtcgccc gagattgaat gcatcattgt cgagagtatc gatcccgaag ggcccttcgg
     2221 ggcgaaggaa gcgggcgaag gatcgctggc ggcgacgatt ccggcaatct cgaacgcgat
     2281 ctacgacgct gtgggagttc ggttgcggga ggcgccgttc acgccggagc gcgtgctggc
     2341 agcgctgcgc gcgaagaagg gaatgaagag gatcgaaatg accgagtcgg ttgacccgac
     2401 cgctccggac aagtttcgcg aacatggcgg ctcgctgtgg ttccgcggca agggcccgat
     2461 gcggcatccg ctcgatccgg cgcgacaggg cacgaatgct gggtcgcccg cgggaggtgg
     2521 agattgagcc ttcccagctt ccaactgctg cgccccaaga ctcttgatga agccatggcg
     2581 ctgatggcga
//
LOCUS       DASRKK010000287        11708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1194008, whole genome shotgun sequence.
ACCESSION   DASRKK010000287 DASRKK010000000
VERSION     DASRKK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194008"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFC15_06255"
     CDS             complement(<1..260)
                     /locus_tag="VFC15_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HZM09799.1"
                     /translation="MRISREKVNKLAHAVADTLAETESVDFIEDRNTIRQEARRVLEL
                     LMAEEAKIDAAARQKIESQRRTILEGSQEWDILYRKYYNEEV"
     gene            complement(257..535)
                     /locus_tag="VFC15_06260"
     CDS             complement(257..535)
                     /locus_tag="VFC15_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HZM09800.1"
                     /translation="MHFSKEYVGYLAREVTKKLIDGEFIEAHDPQLVRDRVQTALLEE
                     LALEDRINDEVRIILDTYSDEMRQSGANYQEMFKKIKNELVRKYKAVL"
     gene            complement(569..1066)
                     /locus_tag="VFC15_06265"
     CDS             complement(569..1066)
                     /locus_tag="VFC15_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09801.1"
                     /translation="MSSYGTTASSPTSSHAPLHNYWLLAGGYFSLGFAAFQVSAIGWP
                     ARAVRYFGGPAELRTEKPIQYGLLCLAIGVIVAIFGLYALSGAGKFRRLPLLRTVLLF
                     VAAVYLLRGLLVIPQTPIVLTHPDQVRFVVFSGIALCVGVIHLGGLVQLWRRGRPGEQ
                     PQILC"
     gene            1266..1730
                     /locus_tag="VFC15_06270"
     CDS             1266..1730
                     /locus_tag="VFC15_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09802.1"
                     /translation="MSDDLSSLKDDMVAFILGHGMSRFQGYVTEDVHSVMWDPRDNPE
                     SWKDFVELAKKAGCAFLSMDEEVLERDELDYLVQRLQQATYPSDDDIEEARWLRSFVG
                     KTGFLQLGWPHQGIMFLYEVSTEWYERYQRLLDLVEDFGTLTVDDNDQDEER"
     gene            1731..3497
                     /gene="recJ"
                     /locus_tag="VFC15_06275"
     CDS             1731..3497
                     /gene="recJ"
                     /locus_tag="VFC15_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZM09803.1"
                     /translation="MRWHLRQADPQDIERLTREAGLSPVIARLLAARGITTAAQAQEF
                     LAPSLSHLHSPYLMTGLRTAVDRLRAAIERGETIFIYGDYDVDGTTAIVILKAALEIC
                     GGKVEYHVPHRIREGYGMRDEVIEQAAAAGIRLIISVDTGIRAFAAADAAARCGVDLI
                     VTDHHLPQAEGVPKALAVVNPNQHGCEYPCKSLCGAGVAFKIAQALLEDAGRASLLPS
                     FLKIVAIATIADAVPLVGENRVFAKLGLQGLRNPRNAGLRALLANCQLDGSRPLSAGD
                     IAFRVAPRLNAAGRMDLAERVIELFTEKDEARAAEMAARLNELNGDRQVEEQRILDEI
                     FRQIDSTPEWKDAYSLVVVGDNWHRGVIGICATRVVERYHRPALVFACDSGEAYGSGR
                     SIQAFHLLDALESCPELFSRYGGHSHAVGCTLPVERLPELRSRLEAYARQRLVPEDFV
                     PVLEYDGEVSLDDVNQGFWESLARLEPFGSGNPTPVFVARGVKLVQPPRIMKEKHLKL
                     RVAGADANGNGKLQRAQEVVGWRMAERAASESLLLGDVLDLAFTVDYNTHPDFGGVQL
                     TLTDFVRPVEVPTLAAPESRRA"
     gene            3543..4184
                     /locus_tag="VFC15_06280"
     CDS             3543..4184
                     /locus_tag="VFC15_06280"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZM09804.1"
                     /translation="MAVAIQQVCNSCGNPALPDGKFCLFCGDVLAESPEKAFQIADLS
                     NSNAGSEAASPEPPEYAGFWLRVWAGAIDVCIEAIGALLFSLLVYYAILLVIDPSYGL
                     THATAAYVSGIAAIAFLTIEAWLYCAFTESSSHRATLGKPIVGLQVVTASGDKLSFGQ
                     ATVRHFMKFLSLFTAAVGFMMAGWTKRRQALHDMPSDCVVIRVPPERFSILRR"
     gene            4257..6617
                     /gene="lptC"
                     /locus_tag="VFC15_06285"
     CDS             4257..6617
                     /gene="lptC"
                     /locus_tag="VFC15_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZM09805.1"
                     /translation="MLQSIAQVRKWLVGFAVALLLIVTASYFIAKAQLKSQFHSVPKQ
                     LGIDIQQTSEGFSLSKSEGGRTIYTIRASNAVQFKKGGHADLKNVHIVVYGKAHDRFD
                     QIYGKEFTYDPQSGDVNAIGEVNIDLQGYAEGPSKPDQAPPEELKNPIHITTNSLTFN
                     QKTGIAHTDDMVEFCTLQASGTAKGAYYESTTNQLQLKSDVHIVTTGDNAADIFGSSG
                     TIQKDPRQAVLLDAKIYEPNRTLTTDKLTMFFEPDNSIQHALAEGNVNIEVRGPTIVD
                     ISGPHGDLNMGPQNAVLQAIVSGGAKYNTRGENLSRGSADTFILDFEADNQPKFFHMV
                     KNARMRQDPQPGKPGSPGQPMEVAADQLNFVLENGNELKTGDTVGKAQITIFPSPPGA
                     KPAKSAPKTGQDFGSGNATTVATAGKFHATFGEDNRMQTLHGTPDARIVSTSPGEPEK
                     VSTSQNLDVTFAPDGGAEKLVQTGDFAYHEPSSKPDTGGRAMFADKATYTPADQILVL
                     NGSPRAIDGGMTTTADGIRLNRQSGEAFADGNVKTTYSDLKPQPDGALLAASDPVHVT
                     SLHMTSRNQPSVAHYSGNVRLWQTANVVRAPLIDFDQQNRTLVAHADTAQSVSSLFVQ
                     QGQDGKLTPVDVTADKLTYVDAERRARYTGKVLAKSTDGTISAEQIDVYLKPADSSGA
                     NQTGAQAKASQPAIPGSDGPSKIDHMVAVGKVVITEPNRRGVGDRLVYTADDGKYYLT
                     GKSASIFDAVHGTTWGDSLTFYSHDDRVLVEGKHSSPTVTRARITK"
     gene            6618..7346
                     /gene="lptB"
                     /locus_tag="VFC15_06290"
     CDS             6618..7346
                     /gene="lptB"
                     /locus_tag="VFC15_06290"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZM09806.1"
                     /translation="MQTLSADEIGKSYRGRRVVNGVSLNVKQGEVVGLLGPNGAGKTT
                     SFYIIVGLIPSDTGRVVINDQDITDVPMYLRARNFGISYLPQEPSIFRKLTVEDNILA
                     VLEAQPISWHERRERQERLIDQLGLGVIRKNRGYALSGGERRRVEIARCLCISPSFML
                     LDEPFSGIDPIAVDDLQRIIFDLKESGIGVLVTDHNVRETLSVTDRAYIINEGRIFRA
                     GTPEQLGSDPEVKRVYLGESFTLV"
     gene            7631..9358
                     /gene="rpoN"
                     /locus_tag="VFC15_06295"
     CDS             7631..9358
                     /gene="rpoN"
                     /locus_tag="VFC15_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZM09807.1"
                     /translation="MSLRVSQKQILTPGLVQMVSVLALNKLELADMINAELTENPVLE
                     ELEDSVPLLDEVSAAEEKMDRDSSAAVLEEPAKTAEEKDPFEEIDFGTFFSEYLDPGL
                     RTSIEMEDSEKPSFENFLSRPTTLTDHLMWQLGSLHVKEDVYRAAELVIGNLNEDGYL
                     TANEDELLGLIQDEHGVVELPVAAAAQPHHAPSVTQFEAESPHPNGHEPPLATADISS
                     EELAPEAATGSLAQANAAFAARPALVPPRTAPSRVSFSRDALREAIDLVRQLDPIGVG
                     ARDLRECLLAQLHHLKTIERKNGNGNHLAEKQSIDDAILMVDQHLHALQNKNYKEVAK
                     AMGRALDDVMRAMEFIRTLDPRPGLRYNKAETRLIEPDVAFVKQGEEYIIVMNDEEIP
                     QLRLNPAYRRLLSRDAAEKDVRNYVKERYKSAIQLIKNIEQRKQTILRVCYSILARQR
                     DFLDQGIDQLKPMMIKEVAEEIGVHPSTVSRAVANKYAHTPQGVFELRYFFSESVNGP
                     EGGGTSLLILKRRVKKLIEDEDPANPYTDDQITHILQSQGIQVTRRTVAKYREDMRIP
                     NTHQRRVKS"
     gene            9499..10098
                     /gene="raiA"
                     /locus_tag="VFC15_06300"
     CDS             9499..10098
                     /gene="raiA"
                     /locus_tag="VFC15_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZM09808.1"
                     /translation="MNVEILARQYEITPAVRDEVEDGLTKLQKILGENFTSKVILSNE
                     KKRFIAEITVKRRRGHIVGLAESTDMVAAVNQALEHIETQALKSNGRRRATTRKAKDK
                     SWKEPVEADAETQIAVGASATTAVPVVVHKFPSIARTTEAHLVKSSDAIAMRPMTIEE
                     AVKECEFRDREVFVFRDGKGRVKVLHRRKDGKIELIEVP"
     gene            10258..11391
                     /locus_tag="VFC15_06305"
     CDS             10258..11391
                     /locus_tag="VFC15_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM09809.1"
                     /translation="MGPSVWQKNPNPFFRVALLAAGMICILLLGSSVLLAQAERATSA
                     AAAGEPAGLSTDSTTPASGQPQDFLTIGSIGGTVLDATGAPVSGAQVKLTRLATTPVL
                     DTPTAQDGHFSFPNVAPGPFELSIALAGFKTKKVSGNLPAKTDYLAPAITLELAPVVT
                     DVSVRLTTEQIAEEQIKVQEQQKLLAVIPNYYATYVANAAPMNPKQKFELAWKLVIDP
                     SSFVIAGMIAGGEQANNSFPGYGQGAAGYARRFGAAYGDFFIGTYVSNAILPSILKQD
                     PRYFYKGTGTIKSRIRYALAMSVMTKGDNGKWQPNYSGILGSLAGGAISNLYYPEGSR
                     HGFETTLNNTLIGIATGAGVNILQEFVFRKVTPKKPPVDPPTT"
     gene            complement(11514..>11708)
                     /locus_tag="VFC15_06310"
     CDS             complement(11514..>11708)
                     /locus_tag="VFC15_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09810.1"
                     /translation="VMRRADVLGPRLPRMKSLTDVQVALAEVFSALADKRVPLQRASR
                     ILFNLEKAALTLRVPSLSWE"
BASE COUNT         2531 a         3543 c         3378 g         2256 t
ORIGIN      
        1 ttcacttctt cgttgtagta cttgcggtac aggatgtccc actcctgcga gccttccaga
       61 atggtgcggc gctggctttc gatcttctgc cgggcggcag cgtcgatctt ggcttcttca
      121 gccatcagca actccagtac gcggcgggct tcctggcgaa tggtgttgcg atcctcgatg
      181 aagtccacgc tctccgtctc cgccagcgtg tcggcgacgg cgtgcgccag cttgttgacc
      241 ttctcgcggc tgatcctcat agcacggcct tgtacttgcg caccagctcg ttcttgatct
      301 tcttgaacat ctcctggtag ttggcgccgc tctggcgcat ctcgtcggag taggtgtcga
      361 ggatgatgcg gacctcgtcg ttgatgcggt cttcgagcgc caattcctca agcagcgccg
      421 tctggacgcg gtcgcgcacc agttgcgggt cgtgggcctc gatgaactca ccgtcgatga
      481 gttttttggt gacttcgcgg gccagatagc ccacatattc tttggagaag tgcatgcttg
      541 tagaaatcgg ttgtcggaac agtcgatttt aacacaggat ttgcggctgt tctccagggc
      601 ggccgcgcct ccaaagttgc actaagccac ccagatgaat gacgccgacg cacaacgcaa
      661 tgccggagaa cacgacgaac cgaacctggt cagggtgcgt tagcacgatc ggagtctgag
      721 gaatcaccaa caaaccgcgc aacaggtaga ccgccgctac gaaaagcaaa accgttcgca
      781 gcagtggcaa tcgtcggaac ttgccggcgc ccgacagagc gtaaagaccg aaaatcgcaa
      841 cgatcacccc tatcgcaagg cacagcaggc catattggat cggcttttct gtcctcaatt
      901 ctgcagggcc gccgaaatac cggaccgcac gcgccggcca ccctattgca ctcacctgaa
      961 atgcggcgaa tcccagcgag aaatacccgc ccgcgagcaa ccagtaattg tgcagcggag
     1021 cgtgcgatga cgttggtgag gatgctgtgg ttccgtagct actcataagc ttcctccatc
     1081 accgcttagg aaaaacgaga gtatcgacaa aaaactagca ccacaggtca tcctccgtct
     1141 gtgatgcggc ctcgcgtctt taccggatcc gagccttccg tttgcctcgc cctgcccgct
     1201 tcggagtatg atgaacattc gctgtggcgc ggcgacgcat cgccctgcag caatgggaaa
     1261 ttaccatgtc tgacgatcta tccagcctga aagacgatat ggtggcgttc attcttggcc
     1321 acggcatgag ccgcttccag ggatacgtca ccgaagacgt gcacagcgtg atgtgggacc
     1381 cgcgcgacaa tcctgaaagc tggaaagatt ttgtcgagct ggccaagaag gccggttgcg
     1441 ccttcctctc catggacgag gaagtgcttg aacgcgacga actcgactac ctcgtgcaac
     1501 ggctgcaaca ggccacctat cccagcgatg acgacatcga ggaagcccgc tggctgcgca
     1561 gtttcgtggg caagaccgga ttcctccaac tgggctggcc gcaccaggga atcatgtttc
     1621 tttacgaagt ctccaccgag tggtatgagc gctatcagcg cctgctcgac ttggtggaag
     1681 atttcggcac gctgaccgtc gacgataacg atcaggacga agaacgttaa gtgcgctggc
     1741 acctccgcca agctgatcct caggacatcg agcggctgac tcgcgaggcc ggactttcgc
     1801 cggtcatcgc tcgtttgctc gccgcccgtg gcatcaccac cgcagctcag gcgcaggaat
     1861 tcctggcgcc atcgctttct cacctgcact cgccctacct gatgaccgga ctgcgcaccg
     1921 ctgtcgaccg gctgcgtgcc gccatcgagc gcggcgagac catctttata tatggcgact
     1981 atgacgtaga tggcaccacc gccatcgtga tcctcaaggc cgcgctggaa atttgcggcg
     2041 gcaaggtcga gtatcatgtg ccgcaccgta tccgcgaagg ctacggtatg cgcgacgagg
     2101 tgatcgagca agcggccgcc gctggcattc ggctgattat cagcgtcgac accggcatcc
     2161 gcgcctttgc agccgcggat gctgccgcgc gttgtggggt cgatctcatc gtcaccgacc
     2221 accacctgcc gcaggctgaa ggcgttccca aagcactcgc ggtggtgaat ccgaatcagc
     2281 acggctgcga atatccctgc aaatcgctgt gcggcgcagg cgtcgcgttc aagatcgcgc
     2341 aggcgctttt ggaagacgct gggcgcgcga gtctgttgcc gtcgttcctg aagattgttg
     2401 ccattgcgac tatagcggac gccgtgcccc tggtgggcga gaaccgggtc ttcgccaagt
     2461 tgggattgca gggcctgcgc aatccccgca atgccggact gcgtgccctc ctggctaatt
     2521 gccagctcga tggctcgcgt cccctgagtg ccggggacat cgcatttcgt gtagccccgc
     2581 gcctcaacgc cgccggacgc atggacctgg cagagcgcgt catcgagctg ttcaccgaaa
     2641 aggacgaagc ccgggccgcc gaaatggcag cgcgcctgaa cgaactcaat ggcgaccgtc
     2701 aggtcgagga gcagcgcatc ctcgatgaaa tcttccgcca gatcgactcc acgccggaat
     2761 ggaaagacgc gtactcgctg gtcgtggtcg gcgacaactg gcaccgcggt gtgattggca
     2821 tctgcgctac tcgcgtagtc gagcgctatc atcggccggc gctggtgttc gcctgcgaca
     2881 gcggcgaggc gtacggctcg ggccgctcca tccaggcttt tcatctgctc gacgcgctcg
     2941 agtcttgtcc cgagttgttc agccgctatg gcggacactc gcatgccgtc ggttgcacac
     3001 ttccggtgga acgcctgccc gagctgcgct cgcgtttgga agcctacgcc cggcaacgcc
     3061 tggtgcccga agacttcgtc ccggtactgg agtacgacgg cgaggtttcg cttgatgacg
     3121 tgaatcaagg tttctgggag tcgctggcca ggctggagcc gttcgggtcc ggaaatccga
     3181 cgccggtgtt tgtggcacgg ggagtgaagc tggtccagcc gccccgcata atgaaggaga
     3241 aacatctcaa actgagggtg gccggggcgg acgccaacgg caacgggaaa ttgcaacggg
     3301 cgcaggaagt tgtaggctgg cgcatggccg agcgcgccgc ctcagagtcg ctgctgctgg
     3361 gtgatgtcct cgaccttgcc ttcactgttg actacaacac tcatccggat ttcggcggag
     3421 tgcaactgac gctgaccgac tttgttcgcc ctgtcgaggt gcccacgctc gcggcgcccg
     3481 aaagccgccg agcgtagttt ggctttacat tgaccttact tcggcgatag tattcacgtc
     3541 cgatggcggt tgcgatccag caagtgtgca acagttgcgg caaccccgcg ttgccggatg
     3601 gcaagttctg cctgttttgt ggcgacgtac tggccgagtc accggaaaag gctttccaaa
     3661 tcgccgatct ttccaactcg aacgctggat ccgaggccgc atctccggag ccgccggaat
     3721 acgccggttt ctggttgcgt gtctgggccg gtgccatcga cgtgtgcatc gaggccatcg
     3781 gtgcgctcct gttctcgctc ctggtctatt acgcgattct cctggtgatt gaccccagct
     3841 acggtctgac ccacgccaca gcggcctatg tcagcggtat tgcagccatc gcctttctga
     3901 ccatcgaggc ctggctgtac tgcgctttca ccgagagttc cagccaccga gctaccttgg
     3961 gcaagcccat tgtcgggctc caggtcgtta ccgcctcggg cgacaagctg agcttcggac
     4021 aggctacggt tcgccacttt atgaagtttc tctcgctgtt taccgctgcc gtcggattca
     4081 tgatggcggg ctggaccaaa cgccggcagg cactgcacga catgccttcc gattgcgtcg
     4141 tgatccgcgt acctcccgag cgcttctcca tcctgcgacg ctagccgcct cttctgccgc
     4201 cattttctgg ctccgattgg cctcgctcca accgcaaggg gtaatatcga agccagatgc
     4261 tccaatccat cgcccaggta cgcaaatggc tggtggggtt tgcggtcgct ctcctcctca
     4321 tcgtcacggc ctcgtacttc atcgccaaag cccagttgaa atcacagttt cacagcgttc
     4381 caaagcaact gggaatcgac atccagcaga ccagcgaggg cttctcgctg tccaagtcag
     4441 agggcgggcg caccatttac accattcgcg cctccaacgc cgtgcaattc aagaagggtg
     4501 gacacgccga tctgaagaac gtgcacatcg tggtttacgg caaggcgcac gaccgtttcg
     4561 accagatcta tggcaaggag tttacctacg atccgcaaag tggcgacgtc aacgccatag
     4621 gggaagtgaa tatcgacctc cagggctacg ccgaaggtcc gtcaaagccc gaccaggccc
     4681 cgcccgagga gttaaagaac ccgattcaca tcaccaccaa ttcacttacc ttcaatcaga
     4741 agacaggaat cgcccacacc gacgacatgg tagaattttg cacgctgcag gcctccggca
     4801 ccgcgaaagg ggcctactac gagtccacga ccaatcagct gcagttgaag tctgacgtgc
     4861 atatcgtcac taccggagac aatgctgccg acattttcgg ttccagcggg accatccaga
     4921 aagatccccg gcaggcggtg ctgcttgacg ccaagattta cgagcccaac cgcaccttga
     4981 ccaccgacaa gctcaccatg tttttcgaac ctgacaattc catccagcac gcgctggcag
     5041 aaggtaatgt caacatcgaa gtccgcggac caaccatcgt ggatatcagc ggtccgcacg
     5101 gcgaccttaa catggggccg cagaacgcgg tgcttcaggc cattgtttcc ggcggcgcga
     5161 agtacaacac gcggggagag aacctgagcc gtggttcggc cgacaccttt attctcgact
     5221 tcgaggccga caatcagccc aagttctttc acatggtgaa gaacgcgcgc atgcggcagg
     5281 atcctcaacc cggcaaacct ggttctccgg gacagcccat ggaagttgcc gccgaccaac
     5341 tcaattttgt tttggaaaac ggcaacgaac tcaagaccgg cgacactgtc ggcaaggcgc
     5401 agataacgat tttcccctcg ccgcccggag ccaaacctgc gaagtccgct cccaagaccg
     5461 gacaggattt cggctcgggt aacgcgacca ccgttgccac ggcgggaaag tttcacgcca
     5521 cctttggcga agacaatcgc atgcagacgc tgcacggaac gccggatgcc cgcatcgtct
     5581 ctacgtcgcc cggagaaccg gagaaggtca gcacctcgca aaatctcgat gtcaccttcg
     5641 ctcccgatgg cggcgcagaa aaacttgtac agaccggaga tttcgcctat cacgagccgt
     5701 cctccaaacc cgacacaggt ggacgcgcca tgttcgccga caaagccacc tacacgccgg
     5761 cggaccagat actcgtgctg aacggctctc cgcgcgccat cgatggcggc atgaccacga
     5821 ccgccgatgg catccgcctt aaccgtcagt ctggcgaggc ctttgccgat ggcaacgtca
     5881 agacaaccta cagcgacctc aagccgcaac ctgacggagc cttactcgcg gcctctgacc
     5941 cggtgcatgt gacttcgttg cacatgacct cccgcaacca gccgagtgtc gcccactact
     6001 ctggaaacgt tcgcctgtgg cagaccgcca acgtagtacg cgctcccctg atcgacttcg
     6061 accagcagaa ccggaccctc gtcgcgcatg ccgacacagc ccagtcggtg tccagcttgt
     6121 tcgtacagca aggacaagat ggcaaactca cgccggttga cgtcactgcc gataagctta
     6181 cctacgtgga tgcggaacgg cgggcccgct ataccggcaa agtcctcgcc aagagcaccg
     6241 atggcaccat cagcgccgag cagatcgacg tctatttgaa gccggcggat tcctccggcg
     6301 cgaaccagac tggcgcgcaa gccaaagctt cgcaaccggc gattcccggc agcgacggcc
     6361 ccagcaaaat cgaccacatg gtcgcagtgg gaaaggtggt catcaccgaa cctaaccggc
     6421 gaggagtggg cgaccgcctg gtttatacgg ccgacgacgg caagtattac ctgaccggga
     6481 aatccgccag catttttgat gccgtacacg gcacaacctg gggcgattcg ttgactttct
     6541 atagtcacga tgatagggtt ctggtggagg gcaagcactc ttcgcccacc gtgacacgcg
     6601 cacgtatcac taagtaaatg cagactttat cggcggatga gatcggcaag agctaccgag
     6661 gacgaagggt cgtcaacggc gtcagtctca atgtaaaaca aggtgaagtg gttggtcttt
     6721 tggggcccaa cggagccggg aaaactacca gcttctacat tattgttgga ctcatcccca
     6781 gcgataccgg tcgcgtagtc atcaatgacc aggatattac cgatgttccc atgtaccttc
     6841 gggcacggaa cttcggcatc agttatctgc cgcaggagcc ttcgatcttc cgcaagctga
     6901 cggtcgagga caacattctc gcggtgctgg aagcgcaacc gatttcgtgg cacgaacgtc
     6961 gcgagcgaca ggagcggctg attgaccaac tcggactggg tgtcatccgc aagaaccgcg
     7021 gctacgcatt gtcgggcgga gaacgtcgcc gcgtggaaat tgcgcgctgc ctctgtattt
     7081 cgccgtcgtt catgctgctc gatgaaccat tctcgggaat cgatcccatc gcggtggacg
     7141 atttgcagag aatcattttt gacttgaaag aaagtggcat cggcgtgctg gttacggacc
     7201 ataatgtgcg tgaaactctt tcagtcaccg atcgcgccta cattattaat gagggtagaa
     7261 tattccgcgc cggcacaccc gaacagttgg gcagcgaccc ggaagtgaag agagtttact
     7321 taggcgagag cttcacgttg gtttaagagc gggttcagtt ttgcgatagt gcaacaggcc
     7381 gcttgagtgc gggaccatcg gtgcgaagat gcgagcggcc gctcccgatc tcagccccgg
     7441 aggggcgaaa taccatagtc cagtccgccg cggcgggctg ggtacgggac agaaaaggcc
     7501 ccgagtcccg gtagggacga cagatgtcag cgcggactta agtccgcgca tgcagattta
     7561 ggtttagaat ttcggcagga ggaacaggtc cccccagggt tccgtagtca tggtcttgtt
     7621 gcagcccaag ttgagtctcc gtgtctctca gaagcaaatt ctgactccgg ggctggtgca
     7681 gatggtcagt gtcctcgccc tcaacaagct tgagctggcg gacatgatta atgcggaatt
     7741 aacggagaac ccggtgctcg aggaactgga agattcggtt cctttactcg acgaagtatc
     7801 ggccgcggaa gagaagatgg atcgcgacag ctcggccgcc gtcctggaag agccggccaa
     7861 gaccgccgaa gaaaaagacc ccttcgaaga aatcgatttc ggaacgttct tctcggaata
     7921 ccttgatccc ggccttcgca cctcgatcga gatggaagac agcgagaagc catcgttcga
     7981 gaacttcctg tcccgtccca caactctgac cgaccacctt atgtggcaac tgggatcgct
     8041 gcacgtgaaa gaagacgtgt atcgcgccgc cgaattggtc ataggtaatc tcaacgaaga
     8101 tggctacctg accgctaacg aagacgagtt gctcggtctg atacaggacg agcatggcgt
     8161 cgtggagtta cccgttgccg ctgcagccca accccatcac gctcccagcg tcacgcaatt
     8221 cgaagctgaa tctccccatc ccaacggaca cgagccgccc ctcgcaaccg ccgacatttc
     8281 atcagaagag ttagcgccgg aagctgccac cggatcgcta gcgcaagcca atgctgcctt
     8341 cgccgcgcgt cctgcgctgg tgccgccacg taccgcgcca tcacgcgtgt ccttcagtcg
     8401 cgatgcgctg cgcgaagcca tcgacttagt caggcaactg gatcccatag gagttggagc
     8461 gcgcgatctc cgcgagtgtt tgctggccca gcttcatcac ctgaaaacca tcgaacgcaa
     8521 gaatggcaat ggcaatcatc ttgccgaaaa gcaatcaatc gacgacgcca tcctgatggt
     8581 cgatcagcac ctgcacgcac tgcagaacaa gaattacaag gaagttgcca aggcgatggg
     8641 gcgcgccctt gacgacgtaa tgcgtgcgat ggagttcatt cgcaccctcg atccgcggcc
     8701 cgggctgcgc tacaacaagg ccgagacccg cctgatcgag cccgatgtgg ctttcgttaa
     8761 acagggcgag gaatacatca tcgtcatgaa tgacgaggag attccgcagt tacgcctgaa
     8821 cccagcctac cgtcgcctgt tgagccgcga cgccgccgaa aaagacgtgc gcaactacgt
     8881 gaaagagcgt tacaagtcgg ccatccagct catcaagaac atcgaacagc gcaagcaaac
     8941 catcctgcgc gtgtgctact ccatcctcgc tcgccagcgc gatttcctcg accagggtat
     9001 cgatcagttg aagcccatga tgattaagga agtggcggag gagattggcg tgcacccctc
     9061 gaccgtgagc cgcgccgtgg ccaacaagta tgctcacacc ccgcagggcg tcttcgaact
     9121 acgatacttc ttcagcgaga gcgtgaatgg cccggagggc ggcggcactt cgctgctgat
     9181 cctgaaacgc cgggtcaaga agctcatcga ggacgaggat ccagccaacc cgtacaccga
     9241 cgatcagatc acccacatcc tccagtcgca gggcatccag gtcactcgtc gcacggtcgc
     9301 gaagtacagg gaagacatgc gcatcccgaa cacccaccag cgcagagtga aaagttagca
     9361 gttgtacccg gcgaccgccg gttccgcgtg catcttttgc agcccgcgga tgccagccgg
     9421 caaggaagcc gaagtggtga aacacaccgc ggccccatca agttccaggc aagcgagctg
     9481 cggtgaggag ggagctcaat gaacgtagag atattagcca ggcaatatga aatcactccc
     9541 gccgtacgcg atgaggtgga agacggcctc accaaactgc agaaaatcct gggtgaaaac
     9601 ttcaccagca aagtcatcct gagcaatgag aagaaacgct tcattgctga aatcacggtg
     9661 aagcgccgcc gcggtcacat tgtcggactg gccgagtcta ccgacatggt ggccgccgtc
     9721 aaccaggcgc tcgaacacat cgagacccag gcgctcaaga gcaatggacg ccgtcgcgct
     9781 accacgcgca aagccaagga caagagctgg aaggaaccgg tcgaagccga tgccgaaacg
     9841 caaatcgcgg tcggcgccag cgcgaccacg gccgtgcccg tcgtggtgca taagttcccc
     9901 tccatcgccc gcaccaccga agcgcacctg gtgaaatcca gcgacgcgat tgccatgcgt
     9961 cccatgacca tcgaagaggc ggtgaaagaa tgtgagttcc gcgaccgcga agtcttcgta
    10021 ttccgcgacg gtaagggccg cgtcaaagtg ctgcatcgcc gcaaagacgg caagatcgaa
    10081 ctcatcgaag ttccgtagat tttcaacaca atccagactg tgtaaccggg acaccgcgcg
    10141 gtgtcccgtc tggaccactg caaaccgtcg ttgtgctagt cttgcgaaca acgcgacact
    10201 ttgcggaaca cctcggttct tagtaagtgt tgctttcttg gggagaagac cggacaaatg
    10261 ggcccgagtg tgtggcagaa aaatcccaac cctttctttc gagtcgcttt gctcgccgcc
    10321 ggaatgattt gtatcctttt gctgggttcc tccgtcctgt tagcgcaggc ggagagggcg
    10381 acctctgccg cggcggccgg tgagcctgcg ggtctctcta ccgattcaac cacgcctgcg
    10441 agcgggcagc cgcaggattt cctgaccatt ggaagcatcg gcggaaccgt gctggatgcg
    10501 acgggcgcac cggtttccgg ggcccaggtc aagctcactc gcctggcgac cacgccggtt
    10561 ctggacactc ctaccgcgca ggacggccat ttttcctttc ccaacgttgc gcccggaccc
    10621 ttcgagctaa gcattgcgct ggcgggcttc aagacgaaga aggtgtcggg gaacctgcct
    10681 gccaaaacgg actacctggc gccggcgatc acgcttgagt tggctccggt ggtcaccgat
    10741 gtgagcgtgc ggctgacgac ggagcagatt gcagaagaac agatcaaggt gcaggagcag
    10801 caaaagctgc tggcggtgat tcccaactat tacgcgacct acgtggcaaa tgccgcgccc
    10861 atgaatccga agcagaagtt tgagttggcc tggaagctgg tgatcgatcc gtcgtcgttt
    10921 gtgatcgccg ggatgattgc aggaggagag caggcgaaca actcgtttcc cggctacgga
    10981 cagggcgcgg cgggttatgc gcggcgcttc ggcgcagcct acggcgactt cttcatcggc
    11041 acgtatgtga gcaatgctat cctgccttcg attctgaaac aggacccacg gtatttctat
    11101 aaaggcacgg gcacgatcaa gtcgcgcatt cgttatgccc tggcaatgtc agtgatgacg
    11161 aagggcgata acggcaagtg gcaaccgaac tattcgggga tactgggcag cctggcgggc
    11221 ggggccatct ccaatctgta ttatcccgaa ggcagccgcc acggttttga aacgacgctg
    11281 aacaatacac tgatcgggat tgcgactgga gcaggggtga acatcttgca ggaattcgtg
    11341 ttccgcaagg tgacgccgaa aaagcctccg gtcgatcctc cgaccacgta aacgcgaccc
    11401 ccctcctcct atctgcaaaa tacttgtttt caatgggata gggggatttc cgcgcaaatg
    11461 cctgcgagca gggaagttat aagtaaagtt ctgagccata agaggttgca cggctactcc
    11521 caggacagac ttggcacgcg caaggtcagc gcggccttct ccaggttaaa cagaatgcga
    11581 ctggcgcgct gaagcgggac gcgcttatcg gcgagggcgc tgaagacctc ggcgagcgcg
    11641 acctgaacgt cggtgagaga tttcatgcgc gggaggcggg ggcccagcac gtcggcgcga
    11701 cgcatgac
//
LOCUS       DASRKK010000288         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_118748, whole genome shotgun sequence.
ACCESSION   DASRKK010000288 DASRKK010000000
VERSION     DASRKK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118748"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..2142
                     /locus_tag="VFC15_06315"
     CDS             400..2142
                     /locus_tag="VFC15_06315"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM09811.1"
                     /translation="MTTEGNKVFAQLTDQFVKESLALSPVNASYAGFHQYKDPKTGKV
                     IELDAELDDVSAKAVAEQEAFYRAWRGRFQKETPVASLDSQDAADYRLIDDNIGLSLL
                     EYDHIQSYKHQPQVYVELVGNGLFLPLTQEYASKEVRVGHVISRIGQIPRLLDQAKSV
                     LTDADPIFISTAVEENDGNVSLIDSVAADIPAGSPLKAQYDKVAPAAKQALTDFTAWM
                     QSDLAKRPTNGRDWRLGKDWYGQKFRYVMETSITPEQLLADAEAELKRIRTEMFQTAL
                     PLYKQMYPGQDDYSNLPGRERENKIIRAVIDKISDEHPERDQLIDAVKADLEGIKQFI
                     RDKKIVSLGSRDNLKVIPTPEFERGIYSVAGFHSAPPLEPNAEAQYWVTPIDPKMPAA
                     KAESKLREYNNYTLKWLTIHEALPGHYVQAEHADDVQPPTRRVLRALLGNGAYVEGWA
                     EYIADVMTEEGYLDHSPKFLLIRRKIMLRATANTILDIRMQTMNMTDQQAMDLMLNDT
                     FQTQAEADGKLQRAKLSSTQLPTYFVGSRQWWVLRKKYQAAKGSGFTLEEFHNKALDQ
                     GALPLEYLEKIILP"
     gene            2609..>3204
                     /gene="amrS"
                     /locus_tag="VFC15_06320"
     CDS             2609..>3204
                     /gene="amrS"
                     /locus_tag="VFC15_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZM09812.1"
                     /translation="MKSAVHQARWWEAMDDGRLHCYLCPRHCHIGEGQHGFCFIRKNE
                     HGKLIQLGYGRPAAVALDPIEKKPLNHFFPGTTILSMGTAGCNMGCFFCQNWDISKAK
                     SDQVRSADLSPRDVVELALEHRAPHIAFTYNEPTIWGEYVIDIAREAHEAGINTVMVS
                     NGYITREAFFDIYRYIDAANIDLKAFTEHFYSKVTMTHL"
BASE COUNT          778 a          906 c          866 g          654 t
ORIGIN      
        1 gtggaattgt gaacccacct cgcaaccgaa agggcgagat tggtaaccct tcacctacag
       61 ctagcgcgct cgagttctat cccaacaagt cccaaagcag acttttccac cgcgcttgga
      121 aatcccgcca agtacgcggg attcccactt tcccacagcg acgacggcta gcgtataaga
      181 aaacctaaaa ccggacattt cacttgctac aaaaagcgga cattttaact tgctacgaac
      241 agcacgaacg tgagctgtgt cttggttgtt gtaccaaaac aggatttata cttggcggtt
      301 cttccacaca tgcgaggtcc tatgcgatat gcgtcttttt gcgtaatttt gcttctggta
      361 gtttcttgtg ccgccggagc gcaaggccca aagaaaaccg tgaccaccga aggtaataaa
      421 gtgtttgccc aacttactga ccagtttgtg aaagaatcgc tggccctgtc tccggtcaac
      481 gcttcctacg cgggatttca tcagtacaaa gatcccaaga ctggcaaagt aattgagctc
      541 gatgctgagc tcgacgacgt cagcgccaaa gcggtggccg agcaggaagc gttttatcgc
      601 gcctggcggg ggcgcttcca gaaggaaacc ccagtggcgt cgctcgattc ccaggacgca
      661 gccgactacc ggctgatcga cgataacatc gggctcagcc tgctcgagta tgaccacatc
      721 cagagttaca agcaccagcc gcaggtgtat gtcgagttgg tgggcaacgg gttatttctg
      781 ccgttgacgc aagagtacgc gtcgaaagaa gttcgcgttg ggcacgtgat ctcccgcatt
      841 gggcaaatcc cgcggctgct cgaccaagcc aaatccgtac tgacggatgc agatcccatt
      901 ttcatctcga cggcggtaga ggagaatgat ggcaatgtga gcctgattga ctcggttgcg
      961 gcagacattc cggcaggctc accgctgaaa gcgcaatacg acaaggtggc tccggcggcg
     1021 aagcaggcgt tgaccgattt cacggcatgg atgcagagcg acctggccaa gcgtccgacc
     1081 aatggccgcg actggcggct gggtaaggat tggtacggcc agaaattccg ctatgtcatg
     1141 gagacgtcga tcacgcctga gcagttgctg gccgacgcgg aggccgagct gaagcgaatc
     1201 cggacggaaa tgtttcagac ggcgcttccg ctttacaagc agatgtatcc cggtcaggac
     1261 gactacagca atctgccggg acgcgagcgc gagaacaaga tcatccgcgc ggtgatcgac
     1321 aagatcagcg atgagcatcc cgagcgcgat caattgattg atgccgtgaa ggccgacctc
     1381 gaaggcatca agcagttcat ccgcgacaag aagatcgttt cactgggcag ccgtgacaac
     1441 ctgaaagtaa ttccgactcc tgagttcgag cgtggcatct actcggtggc ggggttccac
     1501 tcggctccgc cgcttgaacc caacgccgaa gcccagtact gggtaacgcc catcgatccc
     1561 aagatgccgg cggccaaggc cgagagcaaa ctgcgcgagt acaacaatta cacgctgaag
     1621 tggctcacca ttcacgaagc cctgccggga cattacgtgc aagccgagca cgctgacgac
     1681 gtccagccgc caacgcgccg tgtgctgcgc gcgttgctcg gcaatggggc ctacgtcgag
     1741 ggctgggccg aatacatcgc cgacgtgatg accgaggaag gctatctcga ccacagtccc
     1801 aaatttctgt tgatccggcg caagatcatg ctgcgggcca cggcgaacac catcctcgac
     1861 attcgcatgc agaccatgaa catgaccgat cagcaggcca tggacctgat gctgaacgat
     1921 accttccaga cccaggcgga agctgacggg aagctgcaac gcgccaagct aagttcgacg
     1981 caattgccaa cctatttcgt cgggtcgagg caatggtggg tactgcggaa gaagtatcag
     2041 gccgcaaaag gcagtggctt cactctggag gagtttcaca acaaggctct cgatcaggga
     2101 gcgctccctc tcgagtattt ggagaagatc attctgccct gattgatgta aaagtcagga
     2161 tggtcattaa atattaatgt tgtgcgtgtg atagagaagt gagtgaggta aacaccttag
     2221 agtttgctaa ttttcggctc cgtttcggtc tggaagcgcc aaaattgtgc acttcccgca
     2281 ccgaaacgga actgattgtg aagaaatgta aaataaatcg actcacccgg cattttgaac
     2341 aactttcttc gcgcaaaagc gtctatagtg gtaacccaaa tcaggtgggc gccgcaaccc
     2401 cgacagcaac cccaataggc gcccaccgcc ccctctaaaa atctccaagt taatcgtggt
     2461 ctgcgatcgc gcatgcgttg aaaagcgtgc aatgaaattc tcgcgcttca tttccaattc
     2521 gcttggttta cgatgcatgc gtcgtagcgg cgttactaca agcttcatgg cggcgtcagc
     2581 gctacaatga acatttaaga tagttgccat gaagtcagcg gttcatcaag cccggtggtg
     2641 ggaggccatg gacgatggcc gtctgcactg ctacctctgc ccgcgtcact gccacatcgg
     2701 agaaggccag cacggcttct gcttcatccg aaagaatgaa cacggcaagt taattcaact
     2761 gggctatggc cgccctgccg cagttgcact cgatcccatt gagaagaaac cgctcaacca
     2821 cttctttccg ggaaccacga ttctttccat gggcaccgcc ggttgcaaca tgggttgctt
     2881 tttctgccag aactgggaca tctccaaagc caagtccgat caagtgcgct ccgccgatct
     2941 ttcgccgcgg gacgtggtcg aactggcgct ggagcatcgc gctccgcaca tcgcgttcac
     3001 ctacaacgag ccgacgatct ggggcgaata cgtcatcgac attgcgcgcg aagcgcacga
     3061 agcgggcatc aacacagtga tggtttccaa cggctacatt actcgcgagg cgttcttcga
     3121 catctaccgc tatatcgacg ccgccaacat cgacctcaaa gccttcaccg agcacttcta
     3181 ctccaaggtc acgatgacgc acct
//
LOCUS       DASRKK010000289         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_412801, whole genome shotgun sequence.
ACCESSION   DASRKK010000289 DASRKK010000000
VERSION     DASRKK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412801"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFC15_06325"
     CDS             <1..943
                     /locus_tag="VFC15_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HZM09813.1"
                     /translation="AVRLDADEFDKALEKLWIHGGAAVDYAENVSRGHDHWRESYLAQ
                     SQQKQQQLNLMLRYTDSNQCRMCALVRHFGDYADSQKPCGICDFCAPEACLGQRFRSA
                     TDFELAAALRLITALRAGGGKSTGKLHAELFPEGHLSRNSFEDVLGAMARLGLARLTD
                     EVFEKDGRSIPYRKAYLGSAAGELDEAAPIALLMKAEVKSGTPSKSKTKRGKKKAAKK
                     QALPQRVPGSKAKAPAAPRAALDSRIEDALRKWRLAEAKRRGVPAFRVFTDQALKAIV
                     ERRPQTAAELLAIPGIGISTVEKYGATLYRLLHQDLR"
     gene            complement(1428..2135)
                     /locus_tag="VFC15_06330"
     CDS             complement(1428..2135)
                     /locus_tag="VFC15_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HZM09814.1"
                     /translation="MNNAATKSFPSINTPLQVVKSAGWVAILATVATVFLLVTLHVLS
                     PEFSPSWRMISEYAFGHYAWVLSLMFLSWGIGSWALAVALWSHVHTTAGKVGLWFLII
                     AGIGEAMASAFDITHPTGHGIAGLLGVIGFPVAALLLSVTLGRNETWRTARRPLLWVA
                     NLSWISVVLLLVTLAIMTMQMTRINGGHLPQHAPKSLPPGVLALDGWADRLIVLSNCA
                     WVLLAARHAIQLRGKQS"
     gene            complement(2187..>2654)
                     /locus_tag="VFC15_06335"
     CDS             complement(2187..>2654)
                     /locus_tag="VFC15_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM09815.1"
                     /translation="TMSKLVLTTEAETHVMVTRRFAAPPEAVYRAHTDPTLLQKWLLG
                     PEGWTMPVCINEAKPGGKIRYEWMDSNGHGFYVTGEYLELEPYSRIVHVERMHLPDPT
                     PDNHVETRFDADGTGTLMPMRMTLPDAQTRAAMLATGMERGMEASYERLERMI"
BASE COUNT          591 a          799 c          769 g          495 t
ORIGIN      
        1 cgccgtgcgg ctggatgcgg atgagttcga taaagcgctg gagaagctct ggattcacgg
       61 cggcgcggca gtggactatg ccgaaaacgt aagccgcggc cacgaccatt ggcgcgaatc
      121 ctaccttgcc caatcccagc aaaagcagca gcaattgaac ctgatgctgc gctacaccga
      181 ttccaaccag tgccgcatgt gcgccctggt gagacatttc ggcgactacg ccgacagcca
      241 gaaaccgtgc gggatatgcg atttctgcgc gcccgaggcc tgcttgggac agcggttccg
      301 gtctgccacc gacttcgaac tggcggctgc gctacgcctg atcacggccc tgcgcgccgg
      361 aggcggcaaa tccaccggca agctgcatgc cgagctgttt ccggagggac acctctcgcg
      421 caattcattc gaggatgtgc tgggggcgat ggcacgcttg ggcctggcgc gcttgaccga
      481 tgaagtgttc gagaaggacg gccgaagcat tccgtaccgc aaggcgtact tggggagtgc
      541 ggccggggag ttggacgaag ccgctcccat tgcgctgctg atgaaggcgg aagtgaaatc
      601 aggaacgccg tcaaagagca agacaaagcg cggcaagaag aaggcagcca agaagcaggc
      661 attgccgcag cgcgtacctg gatcgaaagc caaggccccg gcggcaccac gggcggctct
      721 cgattcgcgg atcgaggacg cgctgcgcaa gtggcggctg gccgaagcca agcgccgcgg
      781 cgtaccggcc tttcgcgtgt tcaccgatca ggcgctgaag gccatcgtgg agcgccgccc
      841 gcaaactgcg gcggaactac tggcgattcc cggcatcggt atcagtacgg tggagaagta
      901 cggcgcgaca ctctaccgcc tgctgcacca agacctccgc tgaaccgctg cgggtgcgcc
      961 ggcccgctga tttcgaatgc ctgctgacga ggcactccgc acgatgagcg tttagggctg
     1021 accgtagcat ccttaacgga cggcgctgac cacgaatctc gactctcacg agaacagcga
     1081 tgtgatcctc gtcacgcgtt gccgtccacc tctgtccggc agatagctga tccgtcaaat
     1141 cgcacggaat catcaacccc attcggggat tgaccgcaaa cagccaattc atgcaaacca
     1201 ttgttttcat caagtgacat tccgcgaaga ccttataccg gcgggagcgg acacattcgt
     1261 agacactggc cgacttgcgg gtactaatct tgcgaaaacg gggtggattg cttccggacc
     1321 gccgaccatc tctccagaat tgctgtgcca actgcacaaa gtcggaatga gggtgtcacc
     1381 ttgaaaatcg aaagtggaac gcccattcat ctgcgactct gcgtacctta ggattgcttg
     1441 ccccgtaact gaatcgcgtg cctggcagca aggagtaccc aggcacagtt cgataagacg
     1501 atcaggcggt ccgcccaccc atctagtgca agcactcccg gtggcagcga tttcggcgca
     1561 tgttggggca aatgacctcc attgatccgc gtcatttgca tcgtcataat tgccagtgtc
     1621 accaggagca ggacgacgct gatccagctt agattcgcga cccaaaggag cggtcttcgt
     1681 gccgtgcgcc aggtttcgtt gcgacccaga gtaacactca gcagcaaggc cgcaacgggg
     1741 aagccgataa ccccaagcag accggcgatt ccatgccctg ttgggtgcgt gatatcaaat
     1801 gcggaagcca tcgcctcgcc aataccagcg atgatcagaa accacaagcc taccttcccg
     1861 gccgtggtgt ggacatggga ccagagggca accgctaacg cccacgagcc gatgccccac
     1921 gacaagaaca tcagcgacag cacccaagca taatgaccga atgcatattc actgatcatg
     1981 cgccaggagg gcgaaaactc aggactcaat acatgcaggg tcacaagcag aaagaccgtc
     2041 gcgacagtcg caagaatggc aacccagccc gctgacttca cgacctgcaa cggagtgttg
     2101 atcgaaggaa atgattttgt cgccgcgttg ttcatctagt cctccagagc atcgctcgca
     2161 catcggacgc aaacgtgatg agtaacctag atcatccttt ccagtcgctc atagctcgct
     2221 tccattccgc gttccatgcc agttgcgagc atggccgcac gcgtttgcgc atcgggcaaa
     2281 gtcatacgca tgggcatcaa tgtccctgtg ccgtccgcat caaacctcgt ctcaacgtgg
     2341 ttgtccggcg taggatcggg caggtgcatc cgctcgacat gaacgattcg gctgtatggc
     2401 tctaactcga ggtattcgcc agtaacgtag aacccatgcc cgttgctatc catccactcg
     2461 taacggatct tgccgcccgg cttagcctcg ttgatgcaga ctggcatggt ccagccctca
     2521 gggccgagca accatttctg cagtaatgtt ggatcggtgt gggcgcggta cacggcttcg
     2581 ggaggcgcgg cgaagcgcct ggtcaccatg acgtgagttt cagcttcggt cgtcagtact
     2641 aacttgctca tcgt
//
LOCUS       DASRKK010000290         9167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_803756, whole genome shotgun sequence.
ACCESSION   DASRKK010000290 DASRKK010000000
VERSION     DASRKK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803756"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFC15_06340"
     CDS             complement(<1..225)
                     /locus_tag="VFC15_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM09816.1"
                     /translation="MENRSSLPFTCEVELSGDTTLVKCHGPVVSETAGELKELVRPMI
                     PKHRRIVVDLTDVNFVDSSGLGTLVGLKVSA"
     gene            complement(314..829)
                     /locus_tag="VFC15_06345"
     CDS             complement(314..829)
                     /locus_tag="VFC15_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09817.1"
                     /translation="MGRKLFVLLAVTVLAASIPAESKTRGPSTPQERARALGYIQSLE
                     QNPLAKDSLDKRMWLTEWIVQVPDFTVAPCCKDLDSLDKVNDTYSNQLRMQAIYSQAA
                     YILRHPNVTSDAEKQAAGLAGALRTYRSIQRFDPSAQYPVLDNLMALEKQGKLLQYVE
                     QQDQQSKCTDN"
     gene            1053..3152
                     /locus_tag="VFC15_06350"
     CDS             1053..3152
                     /locus_tag="VFC15_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM09818.1"
                     /translation="MKRVVHSACPHDCPDACGALITVEDGRATRIQGDPAHPITQGFL
                     CAKVTKYLDRVYSPDRVLYPMRRVGHKGAGGPEAFQRVSWDDALDEIVAKLKRISAEF
                     GPEAVLPYSYGGTIAVLNGGSMDMRFFHRLGASQLLRTICSLTGGDAIVSMYGRKTGT
                     EPEQFRHSKYIIAWGANIHGNNVHLWPFVEEARRNGAKLVVIDPYRTRTARVADWHIP
                     INPGTDVALAMGLMHIIARDGLHDQDYIARYAEGFGELEKRLPRYTPERVSEWTGIPK
                     DDIECLAHEYATMRPAAIRLNYGIQRAQNGGAAVRAVCMLPVITGSWKQVGGGLQLSL
                     SGGFQLNQAAMERTDLMLKSSLGRVARSINMTELGKALTEISDPPVKALVVYNSNPAA
                     VCPEHNLVVRGLRREDLFTVVHEQFFTDTTDYADILLPATTFFEQKDLVKAYGHLYLQ
                     VSQQAIDPVGECKSNVDLFRELALRMGFEDECFRETVDEMIDGALSSGVPQLEGITRE
                     RLEREPQVRLNLHGDGSDKPWLPFANGFPTPTGRARIYDADLIAQGMDPVAEFVAPEE
                     SRHSPLAKQHPLELLARKADNFLNSTFVNLPSLQKMERQHELEINRQDADSRGVKDGD
                     RVRVFNDRGEIFLHARVDGTVSPGVVGAKLGWAKLSSGGVNINVLTSSRLADLSGGAT
                     FYSTLVEVERVPETSST"
     gene            3391..6102
                     /gene="bamA"
                     /locus_tag="VFC15_06355"
     CDS             3391..6102
                     /gene="bamA"
                     /locus_tag="VFC15_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZM09819.1"
                     /translation="MIEEIIIHGNRRIPADTIRARMFTRPGDVYDQAALERDFNSLWN
                     TGYFDDLRIEREQSPKGWRIHVYVKEKPTIREIKYVGLSSVSQSDVLDKFKERKVGLT
                     QESQYDPTKVKRAEVVIKELLAAHGRQFATVRSEVRPIPPAAVGLTFVVKEGPKVKVG
                     KITFEGNKAVKSRELQSAMKNLKPIGIPHSIFLENLFSRTYDSTKLSEDAERVRYYYQ
                     TKGYFKALVSDPKTQIHDTRPPWYWPFSKTPGKAVDITMPVEEGDRYRLKEITFSGYK
                     AVSNTAALRAQFKIKDGDIFDSEAIRKGLDNLRKAYAALGYINFTPVPNTDPDDEKKT
                     ISLKIDLDEGKQFFVRRIEFQGNTTTRDKVIRRELALEEGQVYNGNLWEMSLLRLNQL
                     QYFEVLKPEQDSEIKQNPQDATVDILLKVKEKGKNSIGLTGGVSGLSGGFIGVNYTTN
                     NLFGKGESLSVQVQVGQYQKNETLSFTQPYLRDKPLQFSWTVYHSSYNYNQQQQLAVQ
                     TGQSVNLPASYQALLQNYTQTSTGFTTSLTYPLKRSFKRVGITYAFDVSTLQTYSASS
                     TLYFESLAFRGLSGPNALTGIQTSKVIPNFSYNRIDNPQRPHQGQSFYMATEIAGLGG
                     NTHYFKPVAEWKKFMPMQKGRNTLGFRLQGSWISGYAGDVAPPFNRFYMGGDQDIRGF
                     DIRAISPVVFFPTAVTIPLQNPDGTTVPLDPTNPRRGTYNVTIPVNQIIFPGGDTSIV
                     SNVEYRVPVIGPVTLAPFIDTGMDFITNTSQLRIASTQVLTLNTTNYGCPYLNPNYSC
                     AGTVRIPTSPVLSPIAGTNYQPYMSTGLELQVILPIVNAPFRIYYAYNPLRLDTYVPP
                     VSPITRSMFPLGAAGDYTFANATALYNPAYLLRDPRKTFRFTVATTF"
     gene            6370..7071
                     /locus_tag="VFC15_06360"
     CDS             6370..7071
                     /locus_tag="VFC15_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZM09820.1"
                     /translation="MKRTFASIVLLVLVSMASLAQTSSATPNPAATTTPAVNPNAVKV
                     GVISMQGAIAGSNEGQRDLEAVDKKFEPRRLELQKLNTDIEDLKKQLNTQGDKMNPEA
                     HEALVKSIESKTKTLQRSAEDAQNEYQQQQSEVAQRILQKIAPVITKYVSDNGYGLLI
                     DASAPWPQGPVVVATQAVDITKAVVDAYNVQSGVPAPAKTAGSTTPRPATTPGVTKPA
                     TTPAKPAASGTTKPQ"
     gene            complement(7515..>9167)
                     /gene="lepA"
                     /locus_tag="VFC15_06365"
     CDS             complement(7515..>9167)
                     /gene="lepA"
                     /locus_tag="VFC15_06365"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZM09821.1"
                     /translation="ERGITIKAHSVRMMYPAADGNIYQLNLIDTPGHVDFSYEVSRSL
                     ASCEGALLVVDASQGVEAQTLANAYIAINNGLEIIPVINKIDLPSADIERTKEMIESA
                     IGLDASHALSISAKTGVGVPEVLEAIIQKVPPPKGRADHRLQALIFDSWFDPYRGVVV
                     LARIVEGTIKKGQRIRLMANGNTFDLETLGVLTPKPVEVDELSAGEVGFLIANIKNVA
                     DTGIGDTITDDVHPALEPLPGFEELKPMVFAGLYTVDAHEHTLLREALEKLRLNDASF
                     FFEPESSAALGFGFRCGFLGLLHMEIIQERLEREYNLNLITTAPSVRYKITLTDRSVL
                     EVDNPSRWPDPSEIEKIEEPVIMATILTNEEYVGGILKLVEDKRGKQKAFEYVSSTRV
                     MLTYELPLNEIVMDFYDKLKSVSRGYASLDYHLTGMWESPMLKLDILVGGEPVDALSI
                     IVHKEFAYDRGRALVSKMRQLIPRQMFEVAIQAAIGAKVIARETVAAMRKNVIAKCYG
                     GDISRKRKLLEKQKEGKKRMKRIGRVDIPQEAFLAVLRVDED"
BASE COUNT         1999 a         2608 c         2675 g         1885 t
ORIGIN      
        1 cgcggacact ttcagcccaa ccagcgtacc cagaccggaa ctgtccacaa agttgacatc
       61 ggtgaggtcg accacaatgc ggcggtgttt ggggatcatg ggtctgacca gttccttcag
      121 ttcgcccgcc gtctcgctga ccaccgggcc atgacacttc acaagcgtgg tgtcacctga
      181 cagttccacc tcacacgtga agggaagcga tgatcggttc tccacagttg cctcctcacc
      241 cagcaaatcg gtgacagtat aacaagaaag ggcgcgaatg acgcgccccg ttcctagcaa
      301 gtttcggtga agcttagttg tcggtgcact tcgactgctg gtcctgctgc tcgacgtatt
      361 gcaggagttt gccctgcttc tccagcgcca tcaggttatc gagcaccgga tactgcgccg
      421 agggatcgaa gcgctgaatc gagcggtagg tgcgcagcgc tccggcaagt ccggcggcct
      481 gtttttccgc atcgctggtc acattcggat ggcgcagaat gtaggcggcc tgcgagtaaa
      541 tcgcctgcat acgcaactgg ttcgagtagg tatcgttgac cttgtcaagc gagtcaaggt
      601 ccttgcagca gggcgccact gtgaagtcgg gcacctgcac gatccactcg gtcagccaca
      661 tgcgtttgtc gaggctgtct tttgcgaggg gattctgttc caggctctgg atgtagccga
      721 gcgcgcgcgc ccgctcctgc ggcgtggacg gaccacgcgt tttcgattcg gcgggaatgg
      781 acgccgccag gaccgtcact gcgagtagga caaagagttt acgacccatg gtgccttctc
      841 ccttgtagct tgggcggcca ggctgattgg tgaatgatac tccgactacc ggctatatac
      901 gcaagcagga actaaaaagg tcaaccataa atcgagcggt tcatcccgct tttcgggggc
      961 ttcgtcccag gggattttca atttggagac aaggccgccg gcggcaaggc aaatccggtt
     1021 ctgcgtggga taattacgca aggttgtaac taatgaaacg tgtcgttcac tccgcctgtc
     1081 cgcacgattg tcctgatgct tgcggtgcgt tgattaccgt ggaagacggg cgggccaccc
     1141 gcatccaggg ggaccccgct catcccatca cgcagggttt tctatgcgcg aaggttacga
     1201 aatacctcga ccgggtgtac tcgccggaca gagtccttta tccaatgcga cgcgtcggtc
     1261 acaagggcgc aggtggcccg gaagcctttc agcgcgtttc atgggacgac gctctggatg
     1321 aaattgtcgc caagctgaag cgcatcagcg cagagttcgg tcctgaagcc gtgctgccgt
     1381 actcctacgg cggcaccatt gcagtgctga acggcggctc gatggatatg cgtttcttcc
     1441 atcgtctggg cgcctcgcag ttactgcgga ccatctgctc cctcaccggg ggcgacgcca
     1501 tcgtctccat gtatgggcgc aagaccggca cggagccgga gcagttccgt cactcgaaat
     1561 acatcatcgc gtggggagct aacatccacg gcaataacgt tcatttgtgg ccctttgtcg
     1621 aggaggcgcg gcgcaatggc gcaaagctgg tggtgatcga tccctaccgc acgcgcaccg
     1681 cgagagtcgc cgactggcat attccgatca atccgggcac cgacgtcgcg ctcgctatgg
     1741 gattgatgca catcatcgca cgtgacggat tgcacgacca ggattacatc gcgcgctacg
     1801 ctgaaggatt tggcgagctg gagaagcggc ttcccaggta cacgccggag cgcgtttccg
     1861 agtggacagg cattcccaag gacgacatcg agtgtttggc gcatgagtat gcgactatgc
     1921 ggccggcggc aatccggctg aactacggta tccagcgcgc gcagaacggc ggagccgcgg
     1981 tgcgtgccgt ctgcatgttg ccggtgatca ccggatcgtg gaaacaagtg ggcggcggat
     2041 tgcaactttc gctgagtggc ggctttcagc tcaaccaagc ggcgatggag cgcaccgacc
     2101 tgatgctgaa gagttcgctg ggccgggttg cacgcagcat caatatgacg gagttgggca
     2161 aggcacttac ggagattagc gatcctccgg tgaaggcgct ggtggtttat aactcgaacc
     2221 cggcagccgt ctgtcctgag cacaacctgg tggtgagggg actgcggcgc gaggatctgt
     2281 tcaccgtcgt gcatgagcag ttcttcaccg acaccaccga ctatgctgac atcctgttgc
     2341 cagcgacaac gttctttgag caaaaagacc tggtcaaagc gtacggtcac ctctatttgc
     2401 aggtgtcgca gcaagccatc gatcccgtgg gtgagtgcaa atcgaatgtg gatttgttcc
     2461 gagaactggc attgcgcatg gggttcgagg acgagtgttt ccgcgagacg gtggacgaga
     2521 tgattgatgg cgcgctgtca tccggagtcc cgcaattgga ggggattaca cgcgaacgac
     2581 tggagcgcga gccccaggtg cggttgaact tgcatggtga cgggagcgac aaaccgtggt
     2641 tgccattcgc taacgggttc ccgactccga ccggacgggc gcggatctac gacgcagacc
     2701 tgatcgcgca gggaatggat ccggtcgcgg agttcgtcgc cccggaagaa tcgcggcact
     2761 cgccgctggc gaagcagcat cccctggagt tgttggcgcg caaggccgac aacttcctga
     2821 actcgacctt tgtgaatctg ccgagcttgc agaaaatgga acggcagcac gagctggaga
     2881 tcaaccggca ggacgctgac agtcgtggag taaaggacgg tgatcgtgtc cgggtattca
     2941 acgaccgggg agagattttt ctgcatgcac gggtggacgg cacggtttcg cccggagttg
     3001 tcggagcaaa actgggctgg gccaaactct ccagcggagg cgtcaacatc aatgtcctca
     3061 cctcttcgcg actggccgac ctcagtggtg gtgcaacgtt ctactccacg ctggtcgagg
     3121 tggagcgagt tcccgaaact tccagcacat gaaagggtta gcttgtgcgt ggctgaagaa
     3181 ctgcgggtgg gattcgcttt ctgagcgtcc acccgtaccg tataattacg atttcatcta
     3241 agcacggttg aggttgttcc tatttcttcc caaagccagg taagccctgc ggagaacttc
     3301 cgccgcgggc ggcgcttctg tgccacggcg ctaagtcttc ttttcgcaat catctgtctc
     3361 gcctcaacgt ccgcttgggg tcagcaaggg gtgatcgagg agatcatcat ccatggcaac
     3421 cgccgcattc cggcggacac gattcgcgcg cgcatgttca cccgtccggg cgacgtctac
     3481 gaccaggcgg ccctggaacg ggacttcaac tcgctttgga ataccggcta cttcgatgac
     3541 ctgcggattg agcgcgaaca gtctcccaag ggctggcgca tccacgtata tgtcaaagag
     3601 aagcctacga ttcgcgagat caagtatgtc ggcctcagtt cggtctcaca aagtgacgtg
     3661 ctcgacaagt tcaaggaacg caaggtcggg ctgacgcagg aaagccagta cgatccgacc
     3721 aaggtgaagc gggcggaagt cgtaatcaag gaattgctgg cagcgcatgg acgccagttt
     3781 gcgacggtcc gctcggaagt gcggcccatt ccgccagccg cggtcggcct gacctttgtc
     3841 gtcaaggaag gccctaaggt gaaagttgga aagatcactt tcgagggcaa caaggcagtc
     3901 aagtcgcgcg agttgcagag cgcgatgaag aaccttaagc cgattggtat tccgcactcc
     3961 atcttcctgg agaacctgtt ttcccggacg tacgactcga caaagctgag cgaagacgca
     4021 gaacgcgttc gctactatta ccagaccaag ggctacttca aggcgttggt atccgacccg
     4081 aagacgcaga tccacgacac gcgcccgccg tggtattggc cgttctcgaa gactccgggc
     4141 aaggccgttg acatcaccat gccggtggaa gaaggcgatc gctaccgcct gaaggaaatc
     4201 acgttcagcg gctacaaggc agtgtcgaac acagccgcct tgcgagcgca gttcaagatc
     4261 aaggacggcg acatattcga cagcgaagcg atccgcaagg gattggacaa cctgcgtaag
     4321 gcgtatgcgg cgctgggcta catcaacttc acaccggtgc cgaatactga cccggacgat
     4381 gaaaagaaaa cgatttcgct gaagatcgac ctcgacgaag gtaaacagtt cttcgtgcgc
     4441 cgcatcgaat tccaaggcaa cactacgacc cgcgacaaag tcatccgccg tgaactggcg
     4501 ctcgaagaag gccaggttta caacggaaat ctctgggaaa tgagcttgct ccgcttgaac
     4561 caactgcagt atttcgaagt cctcaagccc gagcaggact ccgagatcaa gcagaacccc
     4621 caggatgcga cggtcgatat cctgctgaag gtgaaagaaa agggcaagaa cagcatcgga
     4681 ctgacgggcg gagtcagcgg cctttcaggc gggtttattg gcgtcaacta tacgaccaac
     4741 aacctcttcg gcaagggcga gtcgttgtcg gtgcaagtcc aggtcgggca gtaccagaag
     4801 aacgagaccc tatcgttcac ccaaccgtac ctgagggaca agcctctcca gttcagttgg
     4861 acggtttacc acagcagtta taactacaac cagcaacagc agttggcggt ccagacgggc
     4921 cagtcggtca accttccagc tagttaccag gcgctactgc agaactacac ccagaccagc
     4981 acaggcttta ccacgtcgct cacctatccg ctgaagcggt cgtttaagcg ggtcgggatc
     5041 acctacgcat tcgacgtctc gacgttgcag acgtacagcg catcatcgac cctgtatttc
     5101 gagagcctgg cgtttcgcgg actgtcgggg ccaaatgcct tgaccggtat tcaaaccagc
     5161 aaagtcatac cgaacttttc ttataaccgg attgataacc ctcaacggcc gcatcaggga
     5221 cagagcttct acatggcgac ggaaattgcc gggttgggcg gcaacactca ctatttcaag
     5281 ccagtcgcgg aatggaagaa gttcatgccc atgcagaagg gccgcaacac gctggggttc
     5341 cgcctgcagg gatcatggat cagcggttat gcgggagatg tggctccgcc ctttaaccgg
     5401 ttctacatgg gcggcgatca ggacattcgt ggtttcgaca tccgcgccat ttctccggtc
     5461 gtgttcttcc cgaccgcggt taccattccg ttgcaaaacc cggacggcac cacggtcccg
     5521 ctggatccga cgaatccgcg gcgcggcacg tataacgtca ctattcctgt gaatcagatc
     5581 atcttccccg gcggtgacac cagcattgtg agcaacgtcg aatatcgcgt accggtgatc
     5641 gggccggtaa ccctcgcccc gttcatcgat accgggatgg atttcattac taacacttcg
     5701 caactccgga ttgccagcac ccaggtgctt acgctgaaca ccacgaatta tgggtgtccg
     5761 tatctcaatc cgaactattc gtgcgctgga accgtgagga ttcccaccag tccggtattg
     5821 agtccgatag cgggaaccaa ctaccagccg tatatgtcga ccggattaga gctgcaggtc
     5881 atcttgccga ttgtgaatgc gcccttccgg atctattacg cctacaatcc gctccgattg
     5941 gacacgtatg tgcctccggt ttctccgatc acgaggtcga tgttcccgtt gggcgcggcc
     6001 ggggattaca ctttcgccaa cgcgacggcg ctgtataacc cggcctacct gttgcgagat
     6061 ccgcggaaga cgttccgatt cacggtggcg acaaccttct aaggcgggaa ctgagacccg
     6121 gctgctgagg cactcacagc cgaagtcttt ggggcaggtg gcgttttatg caatttgacg
     6181 ctgggaagct gtgttcccta taattgaagt atccgcaccg ccgaatcttc cggtcggggc
     6241 tgctacgcag ctgatgctca tgaagtgagt attctcccgg tcgtccagaa ttgtaccgtg
     6301 ctcgtcatcg tgtgcgactg gaacatctga ggaattcccg gagcagttca aggagtcacg
     6361 gtaaatctaa tgaagcgtac gttcgcaagc atagtcctgc tcgttcttgt ctcaatggct
     6421 tcgctggccc agactagttc ggccaccccc aaccccgcgg caaccaccac gcccgccgta
     6481 aatcccaatg cagtcaaagt gggagttatc agcatgcagg gagcgattgc tggtagcaac
     6541 gaaggccagc gcgacctcga ggccgtggac aagaaattcg aaccgcgccg gctcgaactc
     6601 cagaaactca acaccgacat cgaggacctg aaaaagcagc ttaacaccca gggcgacaag
     6661 atgaatcccg aggcccacga agccctggtg aagtccatcg aatccaagac caagacgctg
     6721 cagcgttcgg ctgaagacgc gcagaacgaa taccagcagc agcagagcga agttgcgcag
     6781 cgcatcctcc agaaaattgc gcccgtgatc acgaagtatg tatcggacaa cggctacgga
     6841 cttctgatcg acgcttccgc gccctggccg cagggacccg tggtagttgc aacccaggca
     6901 gtcgatatca ccaaggcagt ggtggatgcc tacaacgtcc agtctggcgt tccggcccca
     6961 gccaagaccg ccgggagcac aacgccccga cccgcgacca cgcccggggt gaccaagccc
     7021 gcaaccacgc cagccaagcc ggcagcgtca ggaacaacca agcctcagta agctgacttt
     7081 ttggaacgca taatgagacc atcctccgag gatggtcttt tttcgtacgt gaacggccag
     7141 gaaaactgtg ctcatcggaa ggttgaaacg gttccggaca gcaagttcag gttgctgatt
     7201 aacccgtatg cggcggttca gggaatcagt ggacgttggc tgtggaaaac tttgtggatt
     7261 acgcactcga ggcctcacca ggattcaaac tcccggtccg ttcacaaaat tgcaacaaaa
     7321 ctagtgcaga gttaacggcg ttctgtttca atgtcttacc cgtcacggta atgtcccctc
     7381 aaattgccgg aaaaagaagt gaatcggatc ggggcagagc agttttgcac aatttccaaa
     7441 ggggctgacc gggagttaca ggcaggagtt gtggccttat ttttcaatgg cggggacaga
     7501 atgcgggcgt tttgttagtc ctcgtcgacc cgcaggaccg ccagaaaggc ctcctgggga
     7561 atgtcgacac ggccaatgcg tttcatgcgt ttcttgcctt ctttttgctt ttccagaagc
     7621 ttacgcttac ggctgatgtc tccaccgtaa cacttggcaa ttacattctt gcgcatagct
     7681 gcaactgttt cacgagcgat gaccttggct ccgatggcgg cctggatggc cacctcaaac
     7741 atctgccggg gaatcaactg cctcatcttc gagaccaggg cgcggccgcg atcgtaggcg
     7801 aattccttgt ggacgatgat cgacagcgcg tccaccggtt cgcctccgac caggatgtcg
     7861 agcttcagca tgggggattc ccacataccg gtgaggtggt aatcgagcga agcataaccc
     7921 cgcgagacgg atttcagctt gtcgtagaag tccatcacga tttcatttaa cggcagctcg
     7981 taggtcagca tgactcgggt ggagctgacg tactcgaacg ccttctgctt gccgcgctta
     8041 tcttcgacca gtttgagaat gccaccgaca tattcttcat tggtgaggat cgtcgccatg
     8101 attaccggct cttcgatctt ttcaatctcg ctgggatcgg gccagcgcga tgggttgtcg
     8161 acttcgagaa cgctgcggtc agtgagcgtg atcttgtagc gcacgctcgg agcggtagtg
     8221 atgaggttga gattgtattc gcgctccagg cgctcctgaa tgatctccat gtgaagcagg
     8281 cccaggaagc cacaacggaa tccgaagccg agcgcggcag aactttccgg ctcgaagaag
     8341 aacgatgcat cgttgaggcg cagcttctcc agtgcttcgc gcagcagcgt gtgctcgtgg
     8401 gcgtccaccg tgtacaggcc ggcgaacacc atcggcttca gttcttcgaa gccgggcaac
     8461 ggttccagcg caggatgcac atcgtcagtg atggtgtcgc cgatgccggt gtcggccacg
     8521 ttcttgatgt tggcgatgag gaagccgact tcgccggcgc tcaactcgtc cacctcgacc
     8581 ggcttgggag tcagcacgcc cagcgtttcc aggtcgaagg tgttgccgtt ggccatcaat
     8641 cggatccgct ggcccttctt gatcgtgccc tcgacgatgc gggccagtac cacgaccccg
     8701 cgatagggat cgaaccagga gtcgaagatc agcgcctgca gcctgtggtc agccctgcct
     8761 ttgggaggag gaactttctg gatgatcgcc tccagcacct cgggaacgcc aacaccggtc
     8821 ttggcgctga tggacagcgc gtgggaagcg tcaagcccga tggcactctc gatcatttct
     8881 ttggtgcgct cgatatcggc gctgggaaga tcgatcttgt taatgacggg aatgatctcc
     8941 agcccgttgt tgatggcgat gtaggcgttc gccagggttt gcgcttccac gccctgcgag
     9001 gcatccacca ccagcaaagc accttcgcag gaggccagcg aacgcgacac ttcatagcta
     9061 aagtccacgt ggccgggcgt gtcgatcaaa ttgagctgat aaatatttcc atcggccgcg
     9121 ggatacatca tgcgcaccga atgcgcctta atggtaatgc cgcgctc
//
LOCUS       DASRKK010000291         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1194669, whole genome shotgun sequence.
ACCESSION   DASRKK010000291 DASRKK010000000
VERSION     DASRKK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194669"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2821
                     /locus_tag="VFC15_06370"
     CDS             <1..2821
                     /locus_tag="VFC15_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09822.1"
                     /translation="AVNAKGFAEASSDVTVQVSNVRDVLVTLRPAAVQQNVNVKAEPS
                     SITTQELDTTDAVNGGVVTGKDLETIPLAARSFANIAYMVPGTEPVEPSDPTKARITA
                     VSTGGSSGLNNQLSVDGADNSDDYIGGFLQNFSPDVIREFAVQTSQANADVGRTTAAA
                     VIISTKSGTNDWHGDFAVYERAAALNARFPIDNPAPEPKQPFSRQNYVGTIGGPIVKD
                     KLWIFTGFEMVNEHASVNYSPASLTQFNALNQLAQEGLIPNTSSIPVPPYVRVPYQSY
                     LGMMRLDWAQSQRSQWFMRYALDNSTTNNALVQQGTLPSTGATTHPNYQNGVIGNTYT
                     FSPSWVGNLTLDTSYLHLTADRNGYLGFALAFPFSSTSKTISGFETYGDNQFVTPITA
                     FPVIRNQEKYQFRYDVSGSVGRHAPKFGIDFIHEPVMSGAFPGQAETTYVLTQNPTFY
                     LTNPEQLTAELNCLPPFDGTNCQTTSTPAGNGNFSQNVQRLGLYAMDSWRVTPQVTVN
                     YGLRYDTTFGLFQASGVSQLLNPGVQTIAGLQLPLPTGAPHDYRKAFAPRLGISYNPD
                     RIEDLVIRAGIGMYYDDLAQNGWATAFQAVNTPSAFQPCTKPGDPGCLPGAANGGQGA
                     VIDPNYHTPYALHVSGGAQYLWHKNWTLSADYVHEQGVHGYRRYEYTAGYTLFSPLFA
                     QDQATQMANVPNLSVFKSDNTSSYNAMMLHLQGNVSRRFNLTANYVLSRAKTWGCVLG
                     ELFDYVNGVCNPLNPFAPGDYGPSGEDVTSRFTLAGVVYVPGGFQISTLIQAETGRPI
                     TLTTPVDVNGLGDPADDRAVINGVQTTLDQFRGTPYIQVDLRVTRPFKVGERWQILPF
                     VEFFNLFNRNNPGANYVTNIAALPTPVNNLYNATAFCPTPACNVPITNPNQLLVPAGA
                     LGDFFGPGTTVGIPFAAQIGVRATF"
BASE COUNT          671 a          988 c          895 g          638 t
ORIGIN      
        1 agcggtcaat gcgaaagggt ttgccgaggc cagttccgat gtgacggtgc aggtaagtaa
       61 cgtgcgcgac gtcctggtga cgctgcggcc ggcggccgtg caacagaatg tcaacgtgaa
      121 ggcggagcct tcctccatca ccacccagga acttgacacc acggacgcgg tgaatggcgg
      181 cgtcgtcact ggcaaggacc tggagaccat tcctctggcg gcccgcagct tcgctaacat
      241 tgcatacatg gtgccgggca cggaaccggt cgagccttcc gaccctacca aggcgcgcat
      301 taccgcggtg tccactggtg gaagctccgg actgaacaac cagctctcgg tggacggcgc
      361 cgacaattcg gacgactaca tcggcggctt cctgcagaac ttttctcccg atgtaattcg
      421 ggaattcgcc gtgcagacct cgcaggcgaa tgcggacgtt ggccgcacta ccgctgccgc
      481 cgtgatcatc agcacgaaga gcggcactaa cgactggcac ggcgacttcg cggtgtatga
      541 gcgggccgcc gcattgaacg cccggttccc catcgataat ccggcacccg agcccaagca
      601 gcccttctca cggcagaatt acgtgggcac gattggcggg ccgattgtga aggacaagct
      661 ctggattttc accgggttcg agatggtgaa cgagcacgcc agcgtcaact acagtccggc
      721 cagcctgacg caattcaacg cgctgaatca actggcgcaa gaggggttga ttccgaacac
      781 gagttcgatt ccagttccgc cgtacgttcg cgtcccgtac cagtcttatc tgggtatgat
      841 gcgactcgac tgggcgcaat cgcagcgttc gcagtggttc atgcgctacg ccctcgacaa
      901 ctccaccacc aacaatgccc tggtgcagca aggcacgctg ccctctaccg gcgcgaccac
      961 gcatcccaat tatcagaacg gcgtcatcgg taacacgtac accttcagtc catcgtgggt
     1021 gggaaatttg acccttgata ccagttatct gcacttgacc gcagaccgta atggatatct
     1081 cggctttgcg ctggcgtttc cattcagctc cacttccaaa accatctccg gcttcgaaac
     1141 ctatggcgac aaccagtttg ttacccccat caccgcgttc cctgtgatcc gcaatcagga
     1201 gaagtaccag tttcgctatg atgtgagcgg ctctgttggc aggcacgctc ccaagttcgg
     1261 catcgacttc attcacgagc cggtaatgag cggcgccttc ccgggtcagg cggagaccac
     1321 ttacgttctc acccagaacc cgacctttta tctgaccaat cctgaacagc tcacggcgga
     1381 gctgaactgc ctgcccccat tcgacggcac gaattgccag accacatcca ctccggccgg
     1441 caacggcaat ttttcccaga atgtgcagcg gctgggcttg tacgccatgg attcctggcg
     1501 agtgacgccg caggtgaccg tcaactacgg gcttcgctac gacacgacct ttggattgtt
     1561 ccaggcttcg ggggtgagcc agttgctcaa tcctggagtg cagaccattg caggactgca
     1621 actgccgctg cccacgggcg cgcctcatga ttaccgcaag gcttttgcgc cgcgtttggg
     1681 aatttcctac aatcccgacc ggattgaaga cctggtcatt cgcgccggca tcggcatgta
     1741 ctacgacgat ctggcgcaga acggatgggc gacggctttc caggcggtga acacgccgtc
     1801 ggcgtttcag ccttgcacca agccgggcga tcccggctgc ctgccaggtg cggcgaacgg
     1861 cggacagggc gcggtaatcg atcccaacta ccacacgcct tacgcgctgc atgtcagcgg
     1921 cggagcgcag tacctatggc acaagaactg gacgctgagc gccgactacg tccacgaaca
     1981 gggcgtccat ggctaccggc gttatgagta caccgcaggc tacacactct tctctccgct
     2041 attcgcgcaa gatcaggcaa cccagatggc taacgtgcct aacctgtccg tcttcaagtc
     2101 ggacaacacg tccagctaca acgccatgat gctgcatctg cagggcaacg tctcccggcg
     2161 cttcaacctc actgccaact acgtgctgtc gagagcgaag acttggggct gcgttttggg
     2221 agagcttttt gactacgtca acggagtctg caatccgctg aaccccttcg ctccaggcga
     2281 ctacggcccg tcgggcgaag atgtcaccag ccgcttcact ctcgcgggtg tcgtgtatgt
     2341 tccgggtggc tttcaaatat cgacgttgat tcaggcggaa actggccgtc cgattactct
     2401 aacgactccg gtggacgtga acgggctggg cgatccggca gatgatcgcg ccgtcatcaa
     2461 cggggtccaa acgacgctcg accagttccg cggaacgcct tatatccagg ttgatctccg
     2521 cgtcacccgg ccgtttaagg tcggagaacg ctggcagatc ctgccgttcg tcgagttctt
     2581 caatctgttc aaccggaaca atccgggcgc caactatgtc accaacatcg cggccctgcc
     2641 gacgccggtg aacaatctat acaatgccac ggcattttgc ccgactccag catgcaacgt
     2701 accaattacg aacccgaatc agttgctggt gcccgccggc gcgctcggcg atttctttgg
     2761 tcccggaacc accgtgggaa ttccgttcgc ggctcagatt ggggtgcgag cgactttcta
     2821 aagcagtttc agaattggtg tagtctggcg agtacgggta gagcggcgct tcagcgccgc
     2881 gttcgctgcg gataaacgaa tcgtttgagc cactgcggtc attgaaaatg accgcagtgg
     2941 ctcaaacctt atggtgcggg tgatcctgga cgccagcctg aaggctggct ctacccggtc
     3001 tacaccaact ctgaaactgg cctaacaggc cgtggtgcaa gtagcggatt tcctgcacgg
     3061 ctggcgacgt agccgggtac gcgaactcac gaagcacttg acaacggacg cggaggtccg
     3121 gcttctgcaa agccgattct gatttatccg accccggaag agtagataaa cgaacaaaac
     3181 aagcatgcca aa
//
LOCUS       DASRKK010000292         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_510899, whole genome shotgun sequence.
ACCESSION   DASRKK010000292 DASRKK010000000
VERSION     DASRKK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510899"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFC15_06375"
     CDS             complement(<1..323)
                     /locus_tag="VFC15_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09823.1"
                     /translation="MGLTIAEGKALLASLQSQIVTEQIQRYVASVKACPECGKAFRTK
                     GYYRSTLRSVYGKVGMRIRRLRKCPCSGSDAESFSTLFTNKSPITPELRYLTAKMAAL
                     LPFRKV"
     gene            636..809
                     /locus_tag="VFC15_06380"
     CDS             636..809
                     /locus_tag="VFC15_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09824.1"
                     /translation="MSLNGLIEAGSADGSFSGWFHILLVVVVAVEMWESVLSISKVCG
                     KGGKRHFRFPGFP"
     gene            complement(850..2082)
                     /locus_tag="VFC15_06385"
     CDS             complement(850..2082)
                     /locus_tag="VFC15_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZM09825.1"
                     /translation="MISVLGAEKLSLLEIEVFLAASQGVRFAGCGRTEIYGWVERLLC
                     HHEYPFQKRAAKGLLRAYIEHMTGFSRAQSTRLIGGYVRLGRIVAKPSGRPRFQRHYT
                     PADIALLATVDEAHERLSGPATRHILKREFEVYGKAEFERLAALSNGHLYNLRQSQGY
                     RLRLCQYEKTRPTAVQIGERKKPAPNGQPGFLRIDTVHQGDSPGGKGIYHINAVDEIT
                     QWEVVLATPRISEAWLMPLLESILQQFPFVILNFHSDNGSEFINKTVAELLEKLLIEQ
                     TKSRPRKSGDNGLVETKNAAIVRKHIGWGHIAPAHAAPINQFYTGFLNPYVNYHRPSA
                     QAEVQIDAKGRKRRLYKLWRTPLETLLSLERPQQFLRPGLSLNALKRVAASMSDTEAA
                     QRMRQAKSSMFEQIRRSA"
     gene            2158..>2440
                     /locus_tag="VFC15_06390"
     CDS             2158..>2440
                     /locus_tag="VFC15_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09826.1"
                     /translation="MRWTRVLAAAEPPSCYPWNVIHGTKTRGFAALALLLAIGVAGCR
                     KEEQAVVNVAQTAVNAEHKAQEAATERDRQRATLAAIPLPTKSLYLDVHE"
BASE COUNT          473 a          698 c          730 g          539 t
ORIGIN      
        1 acctttcgaa acggtaacag tgcagccatc ttggccgtaa gataccgcaa ctctggcgtg
       61 atggggctct tgttcgtgaa cagtgtcgag aagctttccg cgtccgatcc agagcaaggg
      121 cattttctta gtcgccggat acgcatgccg actttcccgt acacagaacg aagagtcgac
      181 cggtaatagc ccttcgtgcg aaatgccttg ccgcattccg ggcaagcctt gacgctggca
      241 acataccgtt gaatctgctc ggttacaatt tgcgactgga gacttgcaag caacgctttg
      301 ccctcagcta tcgtcaggcc cacagtggcc ggagagacct cctccgtccg ctcaatcgag
      361 cccacctcgt gttcgactgg gctgcctgga accacctcca accaccagct tgattgtcca
      421 tttcatccca agagattaac ccaccccaca actttgcaca ctctcggagc tgggagggta
      481 cgcgtctgcg agtggcttca atattcgagg cggtgtacga ctcaaccggt ccggctctgg
      541 ggggaatgcg gcgacgaacg agtcgtattc cgggaccata cactggaagc gcgcgcgctt
      601 gcaatgatct aaaagaagtc ttttccaatc cagagatgag cctgaacggc ctgattgaag
      661 cgggcagcgc ggacggctcc ttttcgggat ggtttcacat attgctcgtg gtcgtcgtgg
      721 cggtggaaat gtgggaatcg gttttatcga tttccaaggt ttgtgggaag ggcggaaaac
      781 ggcactttcg ttttccgggc tttccataaa ccgtcatttc cacggtccac ttccactgcg
      841 gcgcatggtt catgccgatc ttcttatctg ctcgaacatc gagctcttgg cctgtcgcat
      901 gcgttgtgcg gcttcggtat cactcatcga tgcggctacg cgtttgagcg cgttgaggga
      961 aagtccggga cgcaggaatt gctgcgggcg ttcgagcgag agcagcgttt ccagcggtgt
     1021 tcgccacagc ttgtagagac gccgtttacg acctttggca tcaatctgga cttcggcctg
     1081 ggctgagggg cggtgatagt tgacgtaagg gttgaggaag ccggtgtaga actgattgat
     1141 gggcgcggcg tgggcaggtg cgatgtgacc ccagccgatg tgcttgcgta cgatggcggc
     1201 gtttttggtt tccaccaggc cgttgtcgcc actcttgcgt ggcctgcttt tggtctgctc
     1261 gataaggagt ttttccagca gctcggcgac ggtcttgttg atgaactcgc tgccgttgtc
     1321 ggagtggaag ttgagaatga caaaggggaa ctgctggagg atgctttcca gcaagggcat
     1381 caaccaggct tcagaaatgc gcggcgtggc cagaactact tcccactgcg tgatttcatc
     1441 gacagcgttg atgtggtaga ttcccttgcc tccggggctg tcgccctggt gtacggtgtc
     1501 gatgcgcaga aagccgggct gaccgttggg cgcgggcttc ttgcgttcac cgatctggac
     1561 tgcggtgggc cgggtctttt cgtactggca caagcgcagg cggtagccct ggctctggcg
     1621 aaggttatag agatgtccgt tggacagagc tgccagccgc tcaaactccg ccttgccgta
     1681 gacctcgaac tcccgcttga gaatatgccg cgtggccggc ccgctgagcc gctcgtgcgc
     1741 ttcatcgacg gtggccagca gtgcgatgtc cgccggggtg tagtggcgct ggaatcgtgg
     1801 gcgcccggag ggcttggcaa cgatccggcc gaggcgaaca tagccgccaa tcagccgcgt
     1861 ggactgggcc cggctgaacc cggtcatgtg ctcgatgtac gcccttaaca accccttggc
     1921 ggcccgcttc tgaaacgggt actcgtgatg gcagagcagc cgctccaccc agccataaat
     1981 ctccgtgcga ccgcaaccgg cgaaccgcac accctgcgaa gccgctagaa atacctcgat
     2041 ctccaacaaa ctcaacttct ctgctccaag cacgctgatc atccatcctc agcttgcccg
     2101 ctcagactcc ttcaggctca tcttgcgttg gaatcaactc catgcttcag gctcatcttg
     2161 cgttggacaa gagtgttggc agcagcggaa ccgccatcct gctatccttg gaatgtgatt
     2221 cacggcacaa aaacaagagg atttgcggcc ctggcgctgt tgctggccat tggggttgcc
     2281 ggttgccgca aagaagagca ggcggtggtc aatgtggccc agacagccgt caacgccgag
     2341 cacaaggcgc aggaagccgc cactgaacga gaccggcagc gggctacgct ggccgccatc
     2401 ccactgccca ccaagagtct ctacctggat gtgcatgagc
//
LOCUS       DASRKK010000293         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1292460, whole genome shotgun sequence.
ACCESSION   DASRKK010000293 DASRKK010000000
VERSION     DASRKK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292460"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFC15_06395"
     CDS             <1..203
                     /locus_tag="VFC15_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09827.1"
                     /translation="NLDLRLRYETADASNTGLAFQDNLAVIKAIVTKYPEVRDAFSAV
                     IALAVDARGHEYGSVLPMKDIK"
     gene            complement(254..673)
                     /locus_tag="VFC15_06400"
     CDS             complement(254..673)
                     /locus_tag="VFC15_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM09828.1"
                     /translation="MHQLKEDGKQMAKKFSELVARTMSPESQERARVKTRELLAEMAL
                     DELRAALSLTQEHLAETLGVRQSSVSKIIRGQNMYISTLDKVIKAMGGDLEIFVVLPN
                     GKIKLSLEQFRTVANDTSKKRSRDRKKEPDPLAEIAS"
     gene            1149..1397
                     /locus_tag="VFC15_06405"
     CDS             1149..1397
                     /locus_tag="VFC15_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09829.1"
                     /translation="MKSRLRNITVSLEEDVARWARLEAARKETSVSRLLGEILKERML
                     AKDRYERSMRQALARRPFLKTDGQYLSREEAHDRAHLR"
     gene            1375..1818
                     /locus_tag="VFC15_06410"
     CDS             1375..1818
                     /locus_tag="VFC15_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM09830.1"
                     /translation="MTAPIFVDTNVLIYAFDTAALKKQQAAALWRAELWKSRRGRISF
                     QVLQEFYVNVAHKWPAAREEARGEVRNLMAWHPVSMDDALLERSWRIQDRYKLSFWDA
                     AIVAAAKASSCRYLLTEDLQAEQDLDGVVVINPFVRDPSFLSRPQ"
     gene            complement(1850..>3033)
                     /gene="ligD"
                     /locus_tag="VFC15_06415"
     CDS             complement(1850..>3033)
                     /gene="ligD"
                     /locus_tag="VFC15_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZM09831.1"
                     /translation="RRSGGAPSRFEAQARQDHSLHWRLLRRRKKKLLGRDGHGEGLKP
                     EAESIGRKAPMPRVIHPMLATLVDKPFDSQEWLYEVKWDGYRAIAFLESGSLRLVSRN
                     QNDMTAAYPELRSIGDSIAARTAILDGEIVALDEEGRPSFSLMQQRTGVGEGGRRIRR
                     TRDDVPVVYYVFDLLYLDGYNLMQAELEQRKELLASIQAPGGLIRYSDHYIGNGKALF
                     EAASQRELEGIVAKRRSSPYAQKRSREWLKVKIVKRQECVIGGYTDPRGSRENFGSIV
                     LGLYDDQGRLIPVGQAGSGFTEESHAAMWKRLHALETKRSPFLGKVGSTRPVHFVKPE
                     LVAEIKYSEWTHEGQSGGMKMRAPVFQGLRFDKKPEECKFERPASTSVEVKKAEAGDA
                     G"
BASE COUNT          605 a          904 c          823 g          701 t
ORIGIN      
        1 acaatctcga cctgcggctg cgttacgaga ccgccgatgc ctccaacaca ggcctggcgt
       61 tccaggacaa cctggccgtc attaaagcga tcgtgaccaa gtatcccgag gtccgcgatg
      121 cctttagtgc agtgattgcg cttgccgtcg acgcccgcgg gcacgagtac gggtcggtgt
      181 tgccgatgaa ggacatcaag tgagcagcac aaaatggccc cgagcagaat ttggcgcgag
      241 acactattac ttcttaagag gctatctcag ctaacgggtc aggttctttc tttcgatcgc
      301 gggatcgttt cttcgaggtg tcgttagcga cggttcgaaa ctgttcaagg ctcagcttaa
      361 tcttgccgtt cggcaagact acaaaaattt ccagatcgcc gcccattgct ttgatcactt
      421 tgtcgagcgt acttatatac atattctgac cacgaatgat cttggagacc gaagattgcc
      481 ttacgccaag tgtctccgca agatgctctt gcgttagaga cagtgcagcc ctcagttcat
      541 caagggccat ctccgcaagt aactctcggg tcttgactcg agcgcgctcc tgcgactcag
      601 gagacatcgt ccgagctacc aactctgaaa atttctttgc catttgctta ccgtcttcct
      661 tcaactgatg catctacgtg ctaatccaga atcctatagc tggttcattg ctacttttct
      721 aactccgcca agtgcgcatc gtagatgcga tcggcgaccg ggacattttc ttcgtaccat
      781 cgatcatttc ccgttttgtc tcccccaact aagagaattg caactcgtcg gggatcaaaa
      841 gcatagagta tgcgatacgg gcttccttga tgttggatcc gaagctcccg catattcgca
      901 tgcttcgatg tgacgactcc agaggagtac gggaacggca atgtcggacc ccgttcctca
      961 agcaatccga ctacactctt gacatcctct ctctccgcct catctaacga gtcccaccag
     1021 tgctcgaact ccgaactcac ctctatttcc cacagcacgc tgatattata gcctatagtc
     1081 tatatagata caaaagaata tcttttcttt ggatggacac gagactcgtg acacactatc
     1141 tttgtgtcat gaaatctcgt ctgcgaaaca ttaccgtcag cctcgaggaa gacgtcgctc
     1201 gctgggcacg cctcgaagcc gctcgaaaag agacgagcgt ctcgcggctt ctaggggaga
     1261 ttttgaaaga gcgcatgctc gccaaggacc gctatgagcg ctccatgcgg caagcgctcg
     1321 cgcggaggcc cttcctgaag acggatggtc agtatttgtc gcgcgaggag gcccatgacc
     1381 gcgcccatct tcgttgatac caatgttctt atttacgcct tcgacactgc cgccctgaaa
     1441 aagcagcagg cagccgcgct atggcgagcg gaactctgga agagccgccg aggccggatc
     1501 agcttccagg ttctgcagga gttctacgta aacgtcgccc acaagtggcc agccgcccgt
     1561 gaggaggctc ggggcgaggt gaggaacctg atggcgtggc accctgtgtc catggacgat
     1621 gctcttcttg agcgaagctg gaggattcag gaccggtata agctctcatt ctgggacgca
     1681 gccatagtgg cggcggcgaa ggcatcctcc tgccgttacc tgctgacgga ggacttgcag
     1741 gcagaacagg atctggacgg cgttgtcgta attaacccgt tcgttcgcga tcccagcttc
     1801 ctgtctcgtc cgcagtaaag aaatgttctg cggaatgcag attatgtgcc taccctgcat
     1861 cgcccgcttc tgccttcttc acctcgaccg acgtgctggc cggccgctcg aacttgcact
     1921 cctcgggctt cttgtcgaag cgcagtccct gaaatacggg agcgcgcatc ttcatcccgc
     1981 cgctttgacc ttcgtgcgtc cactcggagt acttgatttc ggcaaccagc tcgggcttca
     2041 caaagtggac cggccttgtg cttcccactt tgccgaggaa cggactgcgt ttcgtctcca
     2101 gcgcgtgcag ccgcttccac atggccgcgt gcgactcctc ggtaaagccg ctgcctgcct
     2161 ggcctacggg aatcagccgg ccctgatcgt cgtagaggcc gagcacgatg gagccgaagt
     2221 tttcgcgtga gccgcgcgga tcggtgtaac cgccaattac gcactcctgc cgcttgacga
     2281 ttttgacctt cagccattcc cggctgcgct tttgcgcata aggactgctg cggcgcttgg
     2341 caactatgcc ctccagttcg cgctgtgacg ccgcttcaaa cagcgctttg ccgtttccga
     2401 tgtaatggtc ggagtagcgg atgaggccgc ccggcgcctg aatggaggcc agtagctcct
     2461 tgcgttgttc cagttcggct tgcatcaggt tgtagccgtc aaggtagagc agatcgaaga
     2521 cgtaatacac cacgggaacg tcgtcgcggg tgcgacgaat gcgacggccg ccttcgccaa
     2581 ctcccgtccg ctgctgcatc aggctgaacg aaggccgtcc ctcctcatcg agcgcgacga
     2641 tctcgccatc cagaattgcc gttcgcgctg cgatggaatc tccgatggag cgcagttcgg
     2701 gatacgctgc ggtcatatcg ttctggttgc gcgagaccag gcgcagcgag ccactctcca
     2761 gaaacgcgat ggcgcgatag ccgtcccact tgacctcgta gagccactcc tggctgtcga
     2821 acggcttatc cacgagagtt gcaagcatgg gatggatgac gcggggcatc ggcgctttgc
     2881 ggccaatgct ctctgcttcg ggcttcaaac cttcaccgtg cccgtcgcgg cctaggagtt
     2941 tttttttacg ccgcctgagg aggcgccagt ggagcgagtg gtcttgcctg gcttgcgctt
     3001 cgaagcggct cggcgccccg cccgatcttc ttt
//
LOCUS       DASRKK010000294         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1097363, whole genome shotgun sequence.
ACCESSION   DASRKK010000294 DASRKK010000000
VERSION     DASRKK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097363"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..294)
                     /locus_tag="VFC15_06420"
                     /pseudo
     CDS             complement(1..294)
                     /locus_tag="VFC15_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(376..1914)
                     /gene="ltrA"
                     /locus_tag="VFC15_06425"
     CDS             complement(376..1914)
                     /gene="ltrA"
                     /locus_tag="VFC15_06425"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM09832.1"
                     /translation="MIIGEMQDKLARWSTENKERKFDRLLRLITDRTWLAEAARVTLA
                     SGGARTAGVDGIDKQRMEIDLPAELLRIRNELLAGTYEPLPARRVYIPKASDRKKLRP
                     LGIPSLRDRIVERAMLMAMQPIWESDFHRLSYGFRPERSVHHAIRTVRLQLQDGHGRS
                     TAGRWIIEGDLASYFDTVHHRLLMRAVRKRIADQRFLALLWKLIKAGSVDRGLFRAAS
                     EGVPQGGVLSPLLSNIMLNEFDQWMEANYLSGKVRGDRGAWNLTLKIRRPIAVRENRQ
                     WKPAVAYCRYADDFTLVVKGTKAHAQEVREACRAFLEGKLKLTLNMAKTHIRHVNDGF
                     VFLGHRIIRKRGPRGTMRPVTSIPWTKYRGLAQKLVKELSGNYSVNKIDQVETLNRIL
                     AGWAEFYRFTDYTATVFQRLDRIVFWKFGHWLGRKYQATLKTLCRQWVRAPEPGRAKT
                     WVLPGPDGRGAFREVTLRRLVTSRKGQFKWRLPRGNPYILRDEKPNIFESRYADLVLA
                     MSNT"
     gene            complement(2565..2805)
                     /locus_tag="VFC15_06430"
                     /pseudo
     CDS             complement(2565..2805)
                     /locus_tag="VFC15_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          533 a          886 c          733 g          653 t
ORIGIN      
        1 gcgccacagc cgttcgatga agacgttgtc gatccagcgg cccttgccgt ccatactgat
       61 cgcgacgccg gcgtcctcca ggaccttgat ccactcgtcg ctcgtgaact gcgatccctg
      121 atcggtgttg aagatctccg gccgtccaaa cctcgacatc gcctcctcaa gcgcttcgac
      181 gcagaagtcg gccgtcaacg tgttcgagag cctgaacgtc agcactttgc ggcttgcgac
      241 atccaggatc gcaaccagat acataaagcc gtgtgccatc ggcagtggag tgagagaccg
      301 gacgtagtcg agctattttc cggtctctcc gccccgaacc ggacttgcac gtttccgcgc
      361 atccggctct ccatctcaag tgttgctcat agcaaggacg agatcagcgt aacgcgattc
      421 gaagatattg ggcttctcat cgcgcaggat gtacggattg cctcgcggca gccgccattt
      481 gaactgaccc ttccggctcg tcaccagtcg ccgcagtgtg acttctctga aggctccgcg
      541 accgtcgggc cccggtaaga cccaggtttt ggcgcgcccg ggttctggtg ctcgaaccca
      601 ctgacggcag agggttttga gggtagcttg atatttccgt ccaagccagt gcccgaactt
      661 ccagaacacg atccgatcca atcgctggaa tacagttgcc gtgtagtcgg taaagcggta
      721 aaactctgcc catcccgcga ggatgcgatt cagcgtttcc acctggtcaa tcttattgac
      781 gctgtaattg cccgacagtt ctttaacaag tttctgtgca agacctcggt acttcgtcca
      841 cgggatcgat gttactggac gcatggtgcc gcgtgggcct cgcttgcgga tgatccggtg
      901 tcctagaaag acaaagccgt cgttcacgtg cctgatatgg gttttcgcca tattgagcgt
      961 gagctttagc ttgccttcca gaaaggcccg acaggcttcg cgcacttcct gtgcgtgcgc
     1021 cttggttcct ttcaccacca gcgtaaaatc atccgcgtag cggcagtacg ccaccgctgg
     1081 cttccactgc cggttttccc ttacggcaat gggtcgccgt attttaaggg tgaggttcca
     1141 cgccccccgg tctccacgta ctttcccgct taggtagttc gcttccatcc actgatcaaa
     1201 ctcattgagc atgatgttgg atagcagagg agataggact cccccctgtg gaacgccttc
     1261 gctggcagca cgaaagaggc cacgatcgac actaccggct ttgatgagtt tccacaacaa
     1321 tgccagaaag cgttgatctg caatgcgctt gcgaacggct cgcatcaaaa gccgatgatg
     1381 aacggtgtcg aagtaactgg ccagatcgcc ttctattatc cagcgacccg ctgtacttcg
     1441 tccgtgccca tcctgcaatt gcaacctgac cgttcgtatc gcatggtgga cgctgcgttc
     1501 gggtctaaag ccgtaggata gccgatgaaa atcgctctcc cagatcggct gcatcgccat
     1561 cagcatcgcc cgctccacga ttcgatcacg cagactcggt atgcccaggg gcctcaattt
     1621 cttcctgtca ctcgctttag gaatatacac acggcgcgcc ggtagcggct cataggtgcc
     1681 cgccaacaac tcgttgcgta tcctcaaaag ctctgcgggg agatcgatct ccatgcgttg
     1741 cttgtcgatt ccgtctactc ctgccgttcg cgcgccgcct gaggccaagg tcacgcgcgc
     1801 agcttccgcg agccaagttc tgtcggtaat cagtcgcagg agtcgatcga acttgcgttc
     1861 tttgttctcc gttgaccatc tcgccagctt gtcctgcatt tcaccgatta tcaaaggtct
     1921 tcacctcatt acagtcggct aattcgccgc acaagccact tcagactgct ccccttcgcc
     1981 ctgtgacagg ctttccctgt cgccgactac tacgagagct ccgccaactt ggtcagcctc
     2041 gggggacaca ctcccgtcac atctgttcca agccttccct agttcacatg tctggactca
     2101 atcacacggg cgtggttgcc tatcgcagtc tttgtccttg cttgccgcaa gtcgtcgcgg
     2161 gtggtttggt ctagcaacgc tctctccaaa ccgccgacaa gatcagctaa ctgttgatcc
     2221 tgctttcgtc ggccagtgcg tatttgtcgc tgtccggtac ccgcgtcctg ccggttaagt
     2281 cgtgtcggca ggggtgacat ttcaaccctc agatgcgttt caataggttc gtgttcctca
     2341 accgtcccat gttcagccta gagactcatc ttggcgtaac gacttcgcct caagccccat
     2401 ttcccgcgga cttcgtcacc ttgccagtgt ccggcaagtc accgccgctt cggctcactt
     2461 cctctcgtag cagaagaagg gcatcttcat aactcgatgc attccacaca tgctcaagcg
     2521 ttgaatcccc cacgcggcgg ggaccgacaa cgtctaggcg tacgatacgt gatgtcccaa
     2581 gaaaacagct gatccggtgc gctcgcgcac accgcccgcg gtttgctgcg cttctgggcc
     2641 acgcgttccg ggcgccggtg ggctaactgg ttgtgcgccc gcagcacccg gtagaaggtc
     2701 gattccgagg cgatatagcg ctgctgatcg gccagccgcg gcacgatctg gctcggcggc
     2761 aggtgaccaa attccgccga gttggccacc gccagcagcg tcgcc
//
LOCUS       DASRKK010000295         6169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_706587, whole genome shotgun sequence.
ACCESSION   DASRKK010000295 DASRKK010000000
VERSION     DASRKK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706587"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VFC15_06435"
     CDS             <1..1611
                     /locus_tag="VFC15_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09833.1"
                     /translation="QQQTVSAEGFSGQAFSPFVPHTVRTKETRTCTDCHVSTTGNNNA
                     WLAHVLIQGTGFMNFMGRYIYVANGKGGYHAVAVAEHSEPPAILGSDFQKVAYPANYE
                     EHIKNKSQLKDADHHDGTVLDLQARGEYLYAAMGAGGLRVFDIANIDDKDFSEKMTTA
                     PVSPLGQRFYVKTKYAQAVASPTTLGVDPLRTHRPENEEQSIHLFYGFLYVADKYEGL
                     VIVGNPDLKAKSPGVGTLLDGNPSNNFLKRALAFNPDGTLNGARRIAFAGTYAYVLCD
                     RGLAVVDLDNPLQPKLVAQIGAPDLDDPRSIAVQFRYAFVVDRAGMKVLDITDLAQPV
                     LVPNALVPLVDARNIYVVRTYAYISAGKQGMAIVNVEAPERPQLDQMFDAGGKLNDTN
                     DIKVGMVSSSQFAFVADGHNGLQVVQLFSPQATPNFFGFAPKPVPKLIASYKTKEPAL
                     AISRGIDRDRAVDETGHQLAVFGRRGSRPFNLEEMQRLYLRDGKLFTVTDSPLGPATE
                     PPMQKSAEQSPPPTAGAELQQEGMELRK"
     gene            complement(1669..2982)
                     /locus_tag="VFC15_06440"
     CDS             complement(1669..2982)
                     /locus_tag="VFC15_06440"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM09834.1"
                     /translation="MLSIIVPLVAVGWLLGEKASGNRAIYSSGPVSSAHAVFGQNCNL
                     CHVRGASFSAAVGDKACLACHNAPIHNPQQALTPACSSCHLEHKGKFKMAATTDAACA
                     QCHGDLHVKQGELKFDPHVSDFDNKHPQFLALRRGQYDPGTVNLNHHAHLQPTLRGPD
                     GTVQMQCSDCHRPVGINQPWPYSIAAVPAASQQPVVVPAAETQQQKKRFTETGAGPYM
                     APIRYVNQCAACHTLQFDPLISQPAPHDKPEIVRAFVAEKIKQLVKENPDLVHKPIST
                     GYEDQIEATRNFLRPTRDYLVLPKTPTTPENWIEQRTEIAERLLWRKDCKVCHSQTIG
                     ETDKLPTSVKAVIPARWLLHSEFDHPAHRMMTCESCHSRIAESKQTAEVNLPGIETCR
                     RCHKETGVSQAAAEGRCYECHSYHDWRKEQPLKGKFDLNALRAGK"
     gene            complement(3076..3999)
                     /locus_tag="VFC15_06445"
     CDS             complement(3076..3999)
                     /locus_tag="VFC15_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09835.1"
                     /translation="MLIDSTNRVWIVVTIVALGLGLVVYIPYALVAVQGPRGGSAMGI
                     VYGSIGSALMLFAGLLGARKKFPVWRVGRATSWMRGHLWLGLLSFPFILFHSGFSLGA
                     APLTRTLMVLFIFVIVSGIVGAILQHFLPRVITTRVPMETVYEQIDHVCAQLATEATD
                     AVADLLSALEGDLEHANPQQRAEAASAGTMGGVTFASAIGVDEPFAATLHSFFDNEIK
                     PYLVQHSGRGRVLADKERASAMFQQFRILCPEDSWPRLNDLENICEEKRELDRQRVLH
                     RLLHGWLLVHIPASYALLLLGAVHAVIALRY"
     gene            complement(4004..>6169)
                     /locus_tag="VFC15_06450"
     CDS             complement(4004..>6169)
                     /locus_tag="VFC15_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZM09836.1"
                     /translation="GTARDFPGVKLTIDQREVVVPAGTTIFDAARISGIPIPTLCHQQ
                     NETPVGVCRMCVVDIGQRVYAASCVRQVEPGMVVKTTTEDVAGVRRTLLELLMADHPT
                     PCQRQQLTHDCELEHLASQAGLTASRYPGLPTPRGHDESSASIAVDHDSCILCERCIR
                     GCSEIRHNFVLARRGKGYQAGIAFDENLPMGESTCVSCGECMVSCPTGALVNKKKEIA
                     TDLPGTAVPIEELQLLEMFQNVSGTFLSLNSDAVHKRHFKAGEIICREGDYGSTAFYI
                     LEGTCEVFLSSPMAHVSTKADQGGFFKRLKSSLTGQAHTREARSERAFIPIDAPVDLP
                     YDNPIAELVAGDLFGEMTCMSFYPRSATVRAKTDCTMLEMLRNVLDILQRNKSFRERV
                     ELNYRKRALENHLRSIPLFASLTPEFIDYLRERVELRRYAAGQVLCHEGEHSDSFFLV
                     RLGFVKVSQSHPGGDLVLGYIGRSGFLGEIGLLGAGMYTATCTALDHVEAVRIAAEDF
                     RVMMEQFPAVKQDLQVVAQQRLAENRARLTTIRTAPIDSFLAQGLMEAQSLLVLDLQR
                     CTRCDQCVRACADAHDGVSRLIREGLRLDKYLIATSCRQCKDPLCMVGCPVGSIRRKN
                     SLEMVIEDWCIGCGMCASNCPYGNINMHPFQVQVEDLSRPGRKVAKVQSKATACNLCL
                     DYDEPSCVYACPHDAAHRVNPSQFFAYLARQSDTTLKKG"
BASE COUNT         1315 a         1826 c         1770 g         1258 t
ORIGIN      
        1 cagcagcaaa ccgtctccgc cgagggattc agtggacagg ctttcagtcc gttcgtaccg
       61 catacggtgc gaacaaaaga aacccgtacc tgcaccgatt gccatgtatc gactaccggt
      121 aacaacaacg cctggctggc gcacgtcctc attcaaggga ccggcttcat gaacttcatg
      181 ggccgttaca tctatgtcgc gaacggaaaa gggggatatc atgccgtcgc ggtagcggaa
      241 catagcgagc cccccgccat cctgggcagc gacttccaga aagttgccta tcccgccaac
      301 tacgaagagc acattaagaa taagagccag ctaaaagatg cggaccacca cgacgggact
      361 gtgctcgacc tgcaggcgcg cggagaatat ctctacgctg cgatgggcgc gggcggtctg
      421 cgcgtgttcg acatcgccaa cattgatgac aaagacttct ccgaaaagat gaccactgcc
      481 ccggtttcac cgctgggcca gcgcttctac gtcaagacca agtacgccca ggcggtggca
      541 tcgccgacaa ctctcggggt agatcctctg cgcactcacc gacccgagaa tgaagagcaa
      601 tccattcacc tgttttatgg cttcctctat gtagccgaca aatatgaggg cctggttatc
      661 gtcggcaatc cggacctcaa ggcgaagagt ccgggcgtcg gcactcttct tgacggcaac
      721 ccgagcaata acttcctgaa gagagcctta gcgtttaatc cggacgggac cttgaatggg
      781 gcccgccgca tcgcattcgc cggaacgtac gcctatgtcc tttgcgaccg gggactcgcg
      841 gtggtcgatc tcgacaatcc gttgcagccc aaactcgtgg cacagattgg ggcacccgac
      901 ctggacgatc cccgcagcat cgcagttcaa ttccggtatg ccttcgttgt cgaccgcgcc
      961 gggatgaagg tcctggatat cacggacctg gcgcagcccg tgctggttcc caacgctctc
     1021 gttcccctgg tggatgctcg caacatttac gtggtccgca cctatgcgta catctcggcg
     1081 ggcaagcagg gcatggcgat tgtcaacgtc gaagcgccgg aacggcctca actggaccag
     1141 atgttcgacg caggaggcaa gctgaacgac accaatgaca tcaaggttgg tatggtcagc
     1201 tccagtcagt tcgcgttcgt cgccgatggt cacaatggat tgcaggtagt tcagttgttt
     1261 tcgccccagg cgacgccgaa tttcttcggc ttcgctccca agcccgttcc caaactcatt
     1321 gccagctata aaacgaagga acctgcactg gccatctccc gcggaatcga tcgcgatcgg
     1381 gcggtggatg agaccggaca tcaactcgcc gttttcgggc gcagaggctc ccgccccttc
     1441 aatctcgaag aaatgcagcg gctttatctc cgtgacggca agctcttcac ggtaaccgac
     1501 agtccgctgg ggcctgcgac cgagccgccg atgcagaaaa gcgccgaaca gtctccgccg
     1561 cctaccgcgg gtgcggaatt gcaacaggag gggatggaac tgcgcaaatg agtggcagag
     1621 attgttggca gtttggggcg ctggtgcggc agatgtcgcc gttggacctc acttccctgc
     1681 ccgcaacgcg ttcaagtcga acttgccttt cagcggttgt tccttccgcc aatcgtgata
     1741 actgtggcat tcatagcacc ggccctcggc cgcggcctgc gatactccag tctctttatg
     1801 acatctacgg caggtctcaa ttccgggcag attgacctca gccgtttgtt tgctctccgc
     1861 gatccgggag tggcaagact cacaggtcat catgcgatgg gctgggtgat cgaattcgga
     1921 gtggagcagc cagcgagcag gaattaccgc cttgacggat gttggtagct tgtcagtctc
     1981 gcctatcgtc tggctgtggc acaccttgca atcttttctc cagagcaacc gctcggcaat
     2041 ctcggtcctc tgctctatcc agttttcggg cgttgtcggg gtcttgggaa gaaccaggta
     2101 atcgcgagtg ggacgcagga aattccgtgt cgcctcgatc tggtcctcat atccggtact
     2161 tatgggcttg tgaactaagt ccggattctc tttgaccaac tgctttatct tttccgcgac
     2221 gaaggcgcgc acaatctccg gcttgtcgtg cggcgccggc tgcgatatca gtggatcgaa
     2281 ttgcaaggtg tggcaggcgg cacattgatt cacgtacctg atgggcgcca tgtaaggccc
     2341 cgccccggtt tccgtaaacc tcttcttttg ctgctgcgtc tcagccgcgg gaacgacgac
     2401 tggctgctga ctggcggctg gcaccgcggc gatagagtac ggccaaggtt ggttgatgcc
     2461 gaccggccga tgacaatcgc tgcattgcat ctgcaccgtc ccgtcaggcc cgcgcagcgt
     2521 gggctgcagg tgggcatgat ggttcaggtt gaccgtcccc ggatcatatt ggccgcgccg
     2581 caaagcgaga aactgcggat gtttattatc gaagtccgag acgtgggggt cgaacttcaa
     2641 ctcgccttgc ttaacatgca ggtcgccgtg gcactgcgca caggctgcat cggtggtggc
     2701 cgccatcttg aacttaccct tatgctccag atggcaggag ctacacgcag gagtcaaggc
     2761 ctgttgtggg ttgtgaatcg gcgcgttatg gcaggcgagg cacgccttgt ctcccacggc
     2821 agcgctaaag cttgcgcctc gtacgtggca taggttgcaa ttctggccga acacggcgtg
     2881 tgccgacgac actggaccgc tactataaat cgcgcgatta cccgatgcct tttcccccag
     2941 cagccatccc accgcaacga gcgggacgat tatggagagc acaagccgcc accgccgaaa
     3001 cccatgcagc cgggtgaagt attgcaagtc gattcgtgtc gctaaggttt tggctgttct
     3061 gacgcgtggc acaggtcaat acctcagtgc tattacagcg tgaactgcac ccagaagaag
     3121 caaagcatac gacgcaggta tatgcaccag aagccagccg tgcagcaatc gatgcaatac
     3181 tcgctgccga tccagttcgc gtttttcctc acaaatattc tccagatcgt tcaaccgggg
     3241 ccaggaatcc tcggggcaca ggatccgaaa ctgctggaac atcgcggaag cccgctcctt
     3301 gtcggccaac actcgacccc gcccgctgtg ctggaccaga tacggtttta tctcgttatc
     3361 aaaaaaacta tgcagcgtag cggcaaaggg ttcatcgacc ccgatggcag aagcgaaagt
     3421 aactccgccc atggttccgg cagacgcagc ttctgcacgc tgttgcgggt tggcgtgctc
     3481 caggtcgcct tccagcgccg aaagaagatc ggccacagca tctgttgcct ccgtagccag
     3541 ctgcgcgcag acatgatcaa tttgctcgta aaccgtctcc attggcacgc gagtcgtaat
     3601 cacgcgcgga agaaagtgct gcaagatagc gccgacaatt ccgctgacta tgacgaagat
     3661 gaacagcacc atcagcgtgc gcgtaagcgg agctgcgccc aggctgaacc ctgaatggaa
     3721 gagaatgaat ggaaagctca gcagtcccag ccagagatgc ccgcgcatcc agcttgttgc
     3781 gcgtcctaca cgccataccg gaaacttctt cctggcgccc agaagtcccg cgaacagcat
     3841 cagcgccgag ccgatgctcc catagacaat tcccatcgcg ctgccgccac gaggtccctg
     3901 gacggcaacc agtgcatacg gaatatagac aacgagcccc agcccgaggg ccacgatagt
     3961 aaccacaatc cagacgcgat tcgtggagtc aatgagcaca ctattagcct ttctttagtg
     4021 tggtatcgct ctgccgggca aggtaggcga agaattggct gggattgacg cggtgcgccg
     4081 cgtcgtgcgg acacgcgtac acgcagctcg gctcgtcata gtccaggcag aggttgcagg
     4141 cggtggcttt gctttgcacc tttgcgacct tgcggccagg tcgggaaaga tcttcgacct
     4201 ggacctggaa cggatgcata ttgatatttc cgtagggaca gttgctggcg cacatcccgc
     4261 aaccaataca ccaatcctcg atgaccatct cgaggctgtt cttgcgccgg atcgatccca
     4321 ccggacagcc gaccatgcaa agcgggtcct tacactgccg gcatgaggtc gcaatcagat
     4381 atttgtcgag gcgcaagcct tcgcggatga ggcggctgac tccatcgtgc gcgtcggcgc
     4441 aggcacgcac gcactggtcg catcgggtac agcgttgcaa atcgagcacc agcaggctct
     4501 gtgcctccat caatccctgc gccaggaaag aatcaatcgg cgccgtgcgg atggtagtga
     4561 gccgagcacg attttccgca agtcgctgct gcgccacgac ctgcaagtct tgcttgaccg
     4621 caggaaactg ctccatcatc acccggaagt cttcggccgc aatccggacc gcttctacgt
     4681 gatcaagggc ggtgcaggtc gccgtgtaca tgccggcgcc cagcaacccg atttcaccga
     4741 ggaagcccga ccgcccgatg taacccagaa ccagatctcc acccggatga gactgcgaaa
     4801 ccttgacgaa acccaggcgt acgaggaaga agctgtcgga gtgctcgccc tcgtgacaga
     4861 ggacttgtcc tgctgcatag cgccgcaact cgacccgctc gcgcagatag tcgatgaact
     4921 caggggtaag ggaagcgaac agcggaatgc tccgcaggtg gttttccagt gctcgcttgc
     4981 gatagttgag ttcgaccctt tcgcggaatg acttgttgcg ctgcaagatg tcgagtacat
     5041 tccgcaacat ctccagcatc gtgcaatccg ttttggcgcg gaccgtggcg gagcgcggat
     5101 agaagctcat gcaggtcatc tcgccgaaga ggtcgcccgc aaccaactca gcgatggggt
     5161 tgtcgtaggg cagatcaaca ggagcatcaa tcggtatgaa ggcgcgctca ctcctggctt
     5221 cgcgggtatg cgcttgaccg gtgaggctgg acttcagcct cttgaagaag ccaccctggt
     5281 cggccttcgt cgagacatgg gccattgggc tggagagaaa aacttcacat gtcccctcta
     5341 gtatgtaaaa agcagtggag ccatagtcgc cttcgcggca gatgatttcg ccagccttga
     5401 agtgcctctt atgcaccgcg tcgctgttca ggctgagaaa tgttcccgac acgttctgga
     5461 acatctcgag tagctgtagt tcctcgatgg gtaccgcggt gccggggaga tcggttgcga
     5521 tttccttttt cttgttgacc agcgcgcccg tgggacagga aaccatgcac tccccacaac
     5581 tgacgcaggt cgattcgccc atgggaagat tttcatcaaa agcgatgcca gcctggtagc
     5641 ctttgcccct gcgggcaaga acaaaattgt gccgaatctc gctgcaaccg cgaatgcaac
     5701 gctcgcacag gatgcaggag tcgtgatcga cggcgatcga ggccgaggat tcgtcgtgtc
     5761 cgcgtggtgt gggcaggcca ggatagcggg aggcggtgag gcctgcctgg cttgcaaggt
     5821 gctccaactc gcagtcgtgc gtaagttgct gccgctggca gggcgttgga tggtccgcca
     5881 tcaacaactc caggagggtg cggcgaaccc cagccacatc ttccgtcgtg gtcttcacga
     5941 ccattccagg ttcaacctgt cgaacgcacg atgcggcgta gacacgctgt ccaatatcca
     6001 cgacacacat ccggcaaacg cccaccggag tttcattttg ttgatgacaa agggttggaa
     6061 tgggaatccc ggagatgcgt gcggcgtcga agattgtcgt tccggccgga acaacaacct
     6121 cccgctgatc aatcgtgagc tttacacccg gaaaatccct tgctgtgcc
//
LOCUS       DASRKK010000296         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_999986, whole genome shotgun sequence.
ACCESSION   DASRKK010000296 DASRKK010000000
VERSION     DASRKK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999986"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..1477)
                     /locus_tag="VFC15_06455"
                     /pseudo
     CDS             complement(443..1477)
                     /locus_tag="VFC15_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1861..2901
                     /locus_tag="VFC15_06460"
     CDS             1861..2901
                     /locus_tag="VFC15_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM09837.1"
                     /translation="MHHYVGLDVSLRQTAVCVVDQTGKIMREGMVASDPEVIADFIAK
                     HAPHVARIGLETGATSTWLWTELKKKELPVICIDARHAKAALKMQINKNDRNDAVGIA
                     RIMQCGWYKEVRVKDLDSHAIKALLVSRALLVKMKRDLENQVRGLLKNLGLVIGRAKM
                     TTFWVRASELIADKPALAAAVEPLLKAREALERQIDDLDRKVLAMARNEPDVRRFMTV
                     PGVGPITALCYFATIDDPTRFNKSRNVGAYVGLTTRRYASGEIDRTGRISKCGDAMLR
                     SYLYEAANVLLTRVAKWSALKAWGMRVAKRSSLAKAKVAVARKLAVILHRMWIDGTEF
                     NWSSKEATAQTA"
BASE COUNT          691 a         1047 c          994 g          674 t
ORIGIN      
        1 cggcaccgac tgcggcttcg cccagagccc gttcgcgcag cgcgtccacc ccagcatcat
       61 gtgggcgaag ctgcgttcgt tgtcggaggg cgcaaggctg gccaccgccg agttgtgggg
      121 caagcgcagc gcggcatgat cgtcatcctg atgtccgctt ctctaatcgg ccggttgggg
      181 tcaagcgctt tcagactatc caccgctgca gtgtcgatgt cgcccgcggg ctcgtgcttc
      241 tcttcggaat cggcaccaag gcccttccat catgggattc gaggacgagg cggaacgatc
      301 ttttggtcgg ccttgccatc agacgaacgg caggtccaag cggacatacg atctcacctc
      361 atccatcgtc ccgcgaggga catcattcca ccgctcggtg gagctcgggt tttctcctat
      421 cgtatttgat cctgcatggc tctcatgctg ttacggcttg ttcccgggtc cagcggaact
      481 cggtgccgtc aacccagatg cgatgcatga tcacggccag gcgacgcgcc agggccacga
      541 ccgccttctt cagcccgcgg cgcctggcga tctgcatcgc ccaggccttg agccatgacc
      601 atttcgttgt gcgcaccagc atgatgtggg ccgcttcgta gagcattgcc cgcatcatct
      661 cgtccccgca ctttgatatc gcgccagttc ggtcgatctc gcccgattga tacttggacg
      721 gcgtcagtcc gaacacagcc ccgaccgcct tggagtttct aaagcgggcg ggcacatcga
      781 cggtggcgcg gtaggtcagc gccaccaccg ggccgacacc ggggatcgtc atcagacgcc
      841 ggcaaacatc atcgtcccgc acgatggcca gcaagcggcg gtgcaggatg ccgatctgct
      901 cgcggattgc acgccggaca atgagcagcg gttcgaccag cagagccagg tcagggatgt
      961 tctcgacaag ctctttgatc cgcgcctcga actttaccgt gcccaccacg cccaccttga
     1021 ggccgaagtt gcgcaacgtg gcgcgcaggt cgttctcgat ggcgatggcc ttcgactgca
     1081 gcagcttgcg atgggtcagc agcatgcgca gcttctgact gcgtaacgtc ttcacatgca
     1141 ccggacggta aagcccgact cgcatcatct gcgcgatgcc gcgggcatcg ttgcggtccg
     1201 tcttgttgat ctgcgccttc agcgccgcat gcatgtgccg cgtctcgaca cagatcaccg
     1261 gtagttctgc ttcggcgagc ccactgaata accattgcga cagcggccca gcttccaacc
     1321 ctatccgctc gaagcggtag gcggggttct tcagcaccgc caactatgct tcaggctcgc
     1381 tcgctacctt cacttcccga acgatcctgc ctgtgtcatc cacaatgcag acgctcgtct
     1441 ccttgaccga aacgtccagt ccggcaaaat gctccatgct gcgcttctcc ttctgatgct
     1501 tgaggccgtc accacggacc tcgtttcacc atcagcctga agcgcagcac ccaaattctt
     1561 cagctatcca caagccgccg gccgattacc ccatctttgg gtcttggtcg tgtaaaaact
     1621 ccagcgtccg ccgcgcgcgt agaaacattt tggagaaatt gcacatcgtg agaactgata
     1681 attctgccga cacgcgattc gatgttgagt tggagaattg tattttctac atttttccaa
     1741 tgtatgagtt tttacacatg gggtaattcc ggggctctgc cataaacagg aaccgggaat
     1801 cagctctcca cgtaccgacg aggccaacac acgaaggcct cgcagccatg gaggcttgca
     1861 atgcaccact atgtcggact ggacgtatcg ctgaggcaga ctgccgtgtg tgttgtcgat
     1921 caaacaggca agatcatgcg ggaaggaatg gttgcgtctg atcccgaggt catcgccgat
     1981 tttatcgcca agcacgcgcc gcacgttgcg cgtatcggtc tcgagaccgg cgcaacgtcg
     2041 acctggctgt ggaccgagct taagaagaag gaactgcccg tcatctgcat cgatgcccgg
     2101 catgccaagg cggccttgaa gatgcagatc aacaagaacg accgcaatga tgccgtcggg
     2161 attgcccgca tcatgcagtg cggttggtac aaggaggttc gggtcaagga tctcgacagt
     2221 catgcgatca aagcgcttct ggtcagccgg gcgctgctcg tcaagatgaa gcgcgatctt
     2281 gagaaccaag ttcgcggcct gctcaagaac ctcggcctcg tcattgggcg cgcaaagatg
     2341 accacgtttt gggtgcgcgc ctcggagctg atcgctgaca agccggcgct ggccgccgcc
     2401 gtcgagccgc tgctcaaagc acgtgaggcg ctcgaacggc agattgatga tctcgatcgc
     2461 aaagtcttgg cgatggcacg caatgagccg gacgtgcggc gcttcatgac cgtaccaggt
     2521 gtgggaccga tcacggcact ctgctacttc gcgacaatcg atgatccgac gcggttcaac
     2581 aagtcgcgca atgtcggcgc ctatgtcgga ttgaccaccc gacgatacgc atccggcgag
     2641 atcgaccgga ccggccgcat ctcgaaatgc ggcgacgcca tgctgcgaag ctatctctac
     2701 gaggccgcga atgtcctgct gacgcgggtc gcgaaatggt cggccctcaa ggcgtggggt
     2761 atgcgcgtgg ccaaacgcag cagcctcgcc aaggccaaag tggccgtagc acggaaactc
     2821 gccgtcatcc tgcatcggat gtggatcgat ggcaccgagt tcaactggtc atcgaaggag
     2881 gcaaccgccc agaccgctta aaagagacaa cgagctcccg cccgcaagcg ggaaacgacg
     2941 tccctgccgg gacgtggcga tggtgaggtc gtcccaggct ttgcgatgcc tgcaagggca
     3001 aagcgcgctt tacacattga tccaccaacg tcacccagcg ccatcatgcg gagggcatgc
     3061 ccctaccgcg gagagaacca tggacccggc aaggaacttc aaaaagagaa acttcacgga
     3121 gagcttgacc ccagacccgg aattagagaa caagcctggg aattgccggg cttttgaact
     3181 ggttaattcg ttaacgattg gtaaattacc accagatgcg ggcgctacga ggctgaaggg
     3241 gattgcggta atgaagcgcg ctgcactggt ggctggggtg tcgattgcga cgcttctcgg
     3301 cgccggaccg accgaagttt tggcctggaa cggcaatgcg cggtcctggt ggggctcgca
     3361 agccatctcg ccatccaatc cagccctgcg ggatttcgcc aaatcg
//
LOCUS       DASRKK010000297         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_608285, whole genome shotgun sequence.
ACCESSION   DASRKK010000297 DASRKK010000000
VERSION     DASRKK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608285"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..557
                     /locus_tag="VFC15_06465"
     CDS             282..557
                     /locus_tag="VFC15_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09838.1"
                     /translation="MGLLLARAPVQITTATEHEAGVPGWQDPNAILYRGVRCSRKPVA
                     LMPTRESAESVCAALLGVLTLHEETDDPDGPHTREASKATSERLGAR"
     gene            783..2255
                     /locus_tag="VFC15_06470"
     CDS             783..2255
                     /locus_tag="VFC15_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09839.1"
                     /translation="MTISRRQLLQTACVGPFVARAQTRLDKLLKRDLGRLNFEATTLG
                     LGGQASLQWTPEGVDPVLIILKAFHLGINYFDTSNAYGPSQLNYGKAFRELHLIPGEP
                     GYNQKLRESIFLTSKTGQRWAKGGVPNPKIRNFGNGPAGSHAADDVRRTLSQIFGDGE
                     GNYPKGAYLNLVLCHALSTLEEVEALYTGLDKTDAKAEHIGTLAVLRDLRDGTNLTGL
                     NPKEEKLIRHVGFSGHNSPPVMIEMLQRDTGNLLDGMLVAINANDRLHFNMQYNNIPV
                     AAARKMGLIGMKTFADGSMYGKQPMFSNNPSHVIRTIGNASLPSHPLIQYSLTTPGIG
                     TLIVGTGHIDDDPKKCQLGQNLIAAQIKPTGLSMSDRRAIEKTTATVQDGKTNYFQLP
                     HQDLTPPRDVALSRDAAGGMSIVKLTWQTAYAGDEPISHYEVLRDGQKIGSVNYRPQV
                     DKTAFLFEDKTAAGGSHKYVVATVDAIGRRAEAGELTASA"
     gene            complement(2595..3005)
                     /locus_tag="VFC15_06475"
     CDS             complement(2595..3005)
                     /locus_tag="VFC15_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09840.1"
                     /translation="MVRRLGRCVLTIRLVTPKRPTAAHDKLSTVPETRDPYQTSHTSL
                     RVQCYAGRKADERPVRFQLGDDHHVVDEVVDQWYGLDATFHKVRADDGNLYILKHETT
                     TDEWTLESFRRLQWKRLTPGQSVFESAVAVEDDQ"
     gene            complement(3034..3237)
                     /locus_tag="VFC15_06480"
     CDS             complement(3034..3237)
                     /locus_tag="VFC15_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09841.1"
                     /translation="MSSPRAVEANPALDLLKKAHTQLVERKQNIEAELARLTELTKEL
                     ETVNTQIQALDKTLGVFESRGTA"
BASE COUNT          785 a         1144 c         1137 g          728 t
ORIGIN      
        1 ctgcgcaagg aagtgctgaa agaacgacga tcgcaagcgt taacccacag gccttgcgtt
       61 ttcgtctgga aggagtttgg ctcctcgagc gcgacactac aagaaacgaa aacgcccgcc
      121 tgtgcaagag atgactgtaa gtcattgatt cgaggtatag ttcggcaaac ttcaaggcct
      181 cttttctggc actagaaagc gattgaccgg tttctgcttt gagttcggcg tcggctgccc
      241 aagcgatcct caaagaacgc gcatccactc cgagcggaac cgtgggattg ctacttgcac
      301 gggcacccgt gcagataaca actgcaactg agcatgaagc cggagtgccg ggatggcaag
      361 atcccaacgc gattctgtat cgtggcgtac ggtgctcgcg caagcccgtg gcgctgatgc
      421 cgacgagaga gtcggcagaa tccgtctgcg ccgcgctcct cggtgtcctg acgctgcacg
      481 aagagacgga cgatccggac ggcccgcaca cccgagaagc ttcgaaggcc accagcgagc
      541 gtctcggtgc acggtaggga ttgggattgg gatatccgcc cagcggaaat ccccaaggag
      601 gtatgggctc tcgattcgtc gagagcgccg cagaccgcca aaccaaccca atctatttgg
      661 aggggaaaga cgaattcctg gagtaggcgc gctggccatc ggctccatcg ccagcgctgt
      721 cggccgcatc aaaggccggc aatttcggct cccgaggtat catattcgtt taggagctcc
      781 ccatgacaat ctcccgcaga cagctactcc aaacggcatg tgtcggcccg ttcgtggccc
      841 gcgcgcaaac tcggctcgac aaactgctaa agcgcgactt gggccgcctg aatttcgagg
      901 cgacaaccct gggtctgggc ggtcaggctt ccctgcaatg gacgcccgaa ggcgtggacc
      961 cggtcctcat tattctcaaa gcctttcacc tcggtatcaa ctacttcgac acgtcaaacg
     1021 cttacggtcc cagccagtta aactacggca aggccttccg cgagttgcac ctgatccccg
     1081 gcgaaccggg gtacaaccag aagctgaggg aatccatctt cctgaccagc aagaccggtc
     1141 agcgctgggc caaaggcggc gtccccaacc ccaaaattcg caatttcggc aacggaccgg
     1201 cggggtcgca tgccgccgac gacgtccggc gcacgctcag ccaaatcttc ggtgatggag
     1261 agggcaacta tccaaagggt gcctatctga acctggtgct atgccatgcc ctctccacct
     1321 tggaagaagt agaggctctc tacactgggc tcgacaagac cgacgccaag gccgagcaca
     1381 tcggtacctt ggccgttctg cgcgatctgc gcgatggcac caacctcact ggcctcaatc
     1441 ccaaagagga gaagctgatc cgccacgttg gattctccgg ccacaactcg cctccggtga
     1501 tgatagagat gctgcagcgg gacacgggga acctgctgga cggaatgctg gtggccatca
     1561 acgccaacga tcggctgcat ttcaacatgc agtacaacaa tatcccggtg gctgccgctc
     1621 gcaagatggg tctgatcggc atgaagactt ttgcggatgg gtcgatgtac ggcaagcagc
     1681 ccatgttctc caacaacccc tcgcatgtga tccgcaccat cggcaatgcg tccctcccca
     1741 gccatccgct gatccagtat tcgctcacca cgcctggaat tggcacgctg atcgtgggca
     1801 ccggccacat cgatgatgac cccaagaagt gtcaactggg gcagaatctg atcgccgcgc
     1861 agatcaagcc cactggcctg tcgatgtccg accgccgcgc cattgagaag accactgcca
     1921 cggtccagga cggcaagacc aattatttcc agttgccgca ccaggatttg acgccgccgc
     1981 gggatgtggc cctctcacgg gatgcggccg gcggaatgag catcgtcaag ctgacgtggc
     2041 agaccgccta cgccggcgac gaacccatct cgcactatga ggttttgcgc gacggccaga
     2101 agatcggcag cgtgaactac cgtccacagg tcgacaagac cgccttcctg tttgaagaca
     2161 agactgccgc cgggggcagt cacaaatacg tggttgcgac ggtggacgcg atcggtcggc
     2221 gcgccgaagc gggcgaactg acagcctcgg catgaaggcg atggcgatcg ggcgtaggcg
     2281 cgtctggtgc cgcacgtagc cggacaattc ttctttgcgc cgggaggtgc ctcccttgaa
     2341 tcgacaacat gcccggcgac catcaccaac cagaacgaaa tggagccggt ccgcgcgcct
     2401 ctcgctgcag gtcgggatgg ggggattggg atgtcagtcg gcaggaatcc ctactggtct
     2461 gcggctgatc ggccgcacga tggaagcaca gatggcaact tccatggcga cgatccgacg
     2521 gggaattccc atttactgga agtagcggag gtcggtaatg ctgattactt tccgtggagg
     2581 gatggtatcc ctcctcattg atcgtcttca acggcgacgg cgctctcgaa tactgattgt
     2641 ccgggggtca accgtttcca ttggagtcgc cggaacgatt ccaatgtcca ttcgtccgtg
     2701 gtggtctcgt gtttgaggat gtaaaggttg ccgtcgtccg cacggacctt gtggaacgtg
     2761 gcgtccaggc cataccactg atcgacaact tcgtcgacga cgtgatgatc gtcgccaagt
     2821 tgaaaccgca ccggccgctc gtccgccttc cgtccggcgt agcactggac gcgcaggctc
     2881 gtatgactgg tctgatatgg gtctctcgtt tccggcaccg tgctgagctt atcatgcgct
     2941 gctgtggggc gcttcggggt caccagccgg atggtgagga cgcagcgccc taaccgtcgc
     3001 accaatcgga tgtactcggg aatggtcccc tcgctacgcc gtgccccgtg attcaaacac
     3061 ccccagcgtc ttgtcgaggg cctggatctg cgtattgacc gtctctagct ccttcgtcaa
     3121 ctcggttagg cgtgccagct cggcttcgat gttctgtttg cgctcgacca gttgggtgtg
     3181 cgccttcttg agcagatcga gcgccgggtt tgcctcgact gcccggggag acgacaccag
     3241 ggattcgcac gaggtcaccg ttcgaacagg gccggactac ctgccggtgg ccccgttgcg
     3301 gcggcgcctg gagctattgg cgcggtggtt cccgccgaac cgcggttatc gccagacacc
     3361 cgcggtgccg cgtcgttccg ggccgtcacc gcgtgcctgt taactgctgc tgttctggag
     3421 atcatccggc ttgcgtccgt tgatcgagcg gtggcgttaa ccgcctcggg atttcgcggt
     3481 ggagaccggg agaaacgccc cccagaggac cgttcggagc gcatacttgg ctccccgaga
     3541 ggaacacaga tccgaacacg gttttggaga accagaaatg ctcaagattc tccagcccgt
     3601 taaccaagcg gaagagttcg gagacggacc ttcgttgtgg atagttcagt tgtcaatgat
     3661 cagacagcag gcctaagaat acaccaagcg tcgggttggg gaaacggacg agtttgaagg
     3721 ggagaagctg atcgaggcga ttatcgcgac cttcggtcac cgaggaacga gcatcgaagc
     3781 cgagcccatc ggac
//
LOCUS       DASRKK010000298         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1097737, whole genome shotgun sequence.
ACCESSION   DASRKK010000298 DASRKK010000000
VERSION     DASRKK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097737"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFC15_06485"
     CDS             <1..251
                     /locus_tag="VFC15_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09842.1"
                     /translation="SWMTGINEVAQVVFVRKRMVEVQYLRATITDQQRQEFAIPGRRH
                     RPQYRIGTVPAAQRHPLPPEPLYDLPFYWAMSGAARSD"
     gene            616..1023
                     /locus_tag="VFC15_06490"
     CDS             616..1023
                     /locus_tag="VFC15_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09843.1"
                     /translation="MKGLELAKLARQQGQRFESATWLHKARQMPKAEFQREVEKELTG
                     KDSEPSELIYFKVYKSQIPVIEQAIETAALMLGTDKSRGYCLEMICADFLAGASMDGN
                     NPEILLQSISRYYKFLPGPQQDAFLSEASRKAS"
     gene            1020..1259
                     /locus_tag="VFC15_06495"
     CDS             1020..1259
                     /locus_tag="VFC15_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZM09844.1"
                     /translation="MTKILSRSPRRRLELHRLVLERDGWRCQLCGSMQHLQVHHLKFR
                     SQAGSDEEPNLITLCAECHERTHRSSSRYEYGGRS"
     gene            complement(1320..1637)
                     /locus_tag="VFC15_06500"
     CDS             complement(1320..1637)
                     /locus_tag="VFC15_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09845.1"
                     /translation="MDDKLTIFMAVIAVAIVLQMVFLAALCFILHRLNERLESVTYVT
                     YLVDVMWEMVRARLLPALEETTHILKSSRPKIEVVLDNLVTLASTIHDAALSDSTRNR
                     WPE"
     gene            complement(2052..2477)
                     /locus_tag="VFC15_06505"
     CDS             complement(2052..2477)
                     /locus_tag="VFC15_06505"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HZM09846.1"
                     /translation="MKLLVDECLSDELTKLAQRRGHAEASHIAWVGKSGWKDWELKAV
                     ILDGDWTFVTKNSIDFRGPDDAPGSRGQYADVALHAGLICLNGPVGMDLDLQFELFEA
                     ALDELDCDNDLVNQVLEITLPDPADEIEVLRYKLPADDV"
     gene            complement(2474..3124)
                     /locus_tag="VFC15_06510"
     CDS             complement(2474..3124)
                     /locus_tag="VFC15_06510"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZM09847.1"
                     /translation="MCAVKVQTLTPTEAAVVACVPVRDVNRIIDEKILPEAFYVADRD
                     RARRLYADACAFISFYFRAANRLTSEERMRTIAVASARLREESGSVLEKEWIIREEFL
                     TIDLAPFLRSVHERLARLAAAQALVVEDADIMGGTPVIRDTRIPVYDVAASVAAGLPT
                     ARILAVYPGLTAEAVELASFYAEANPQRGRPRQPASPPAGAVIVSTRRKPRRELAS"
     gene            complement(3238..3363)
                     /locus_tag="VFC15_06515"
     CDS             complement(3238..3363)
                     /locus_tag="VFC15_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09848.1"
                     /translation="MGGYLVHKFVKQTKIDSRLADQASLNQITLVETEPVRHQLL"
     gene            3374..4060
                     /locus_tag="VFC15_06520"
     CDS             3374..4060
                     /locus_tag="VFC15_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09849.1"
                     /translation="MNRRRAQEVLSKIDAILAWEARHENERDTRFVELGKYLCEVRAG
                     QYWRLENLKSFDEFLERRFPESRRKAYYLMSIHEQLPPQVRKELKQVGWTKGLELAKL
                     ARQQGQRFESATWLHKARQMPKAEFQREVERELTGKDSEPSELIYFKVYQSQLPVIER
                     AIEAAALMLGSDKFRGYCLEMICADFLSGASIDGGNPEILLQSISRYYKFLPSPQQEA
                     FLSEVNWKAS"
     gene            4057..4317
                     /locus_tag="VFC15_06525"
     CDS             4057..4317
                     /locus_tag="VFC15_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZM09850.1"
                     /translation="MKKVSRKSPRLRLDANSYLELHRQVLERDGWRCQFCGSMQNLQV
                     HHLKFRSHSGGDEEQNLITMCAECHARMHSKATCRPDNFNGR"
BASE COUNT          968 a         1237 c         1280 g          875 t
ORIGIN      
        1 attcgtggat gaccgggatc aatgaagtcg cccaggtcgt cttcgttcgc aagcgcatgg
       61 tggaagtcca gtatctgcgg gccaccatca ccgaccaaca gcggcaggag ttcgccattc
      121 ctggtcgacg acaccgtccg cagtatcgaa tcgggactgt tcctgccgca cagcggcatc
      181 cgcttccccc agaacccttg tacgacctgc ccttttattg ggctatgtct ggagcagcgc
      241 gatctgattg aggctgggat gattcgcaag ccgggagtcg atcttggtct gtttgacgaa
      301 cttgcttact aagtgtccgc cgatgccgcc caagctgaat cgccgccgcg cccaggcagt
      361 cctgaacagg atcgacgcga tcctggccta ggaggcacgg catgagaacg agcgcgatac
      421 caaggtttgt cgagctcgga aagtacctct gcgaagtgcg ggccggccag tactggcgac
      481 tggagaacct gaagtcattc gatgagttcc tggagcgccg gtttccggag tccagaagga
      541 aagcctacta cctgatgtcg attcacgaac atcttccacc ccaggtgaga aaggagctga
      601 aacaggtggg atgggatgaa ggggctggag ttggccaagc tggcgcgcca gcagggtcag
      661 cgcttcgaaa gtgcaacctg gctgcacaaa gctcgacaga tgcccaaagc ggagttccag
      721 cgggaagtgg agaaggaact gacgggcaag gacagcgaac cttcggagtt gatctatttc
      781 aaggtctata agagtcagat cccggttatt gagcaggcga ttgagactgc agccttgatg
      841 ctggggacgg acaagtcccg gggctactgc ctggagatga tctgtgcgga cttcctcgcc
      901 ggggcctcga tggatggcaa caatccggag atcctgctgc aatccatctc acgttactac
      961 aagttcctgc ccggtcctca acaggacgct tttttgtcgg aggccagccg gaaggcttca
     1021 tgacgaagat cctatccaga agcccgcgac gcagactcga gctgcaccgc ctggtgctgg
     1081 aacgcgacgg ctggcgatgc cagctctgcg gcagcatgca gcacctgcag gtgcaccacc
     1141 tcaaattccg cagccaagcg ggtagcgacg aggaaccgaa cctgatcacg ctatgcgccg
     1201 aatgccacga acggacacat cgaagctcta gccgttacga gtatggcggt cgcagctgat
     1261 ttcactagag tggtgccaac taggtatttc cgcctttacc aatccagcgt aatctcccgt
     1321 cattcaggcc agcggttacg agttgaatcg cttagggcag catcatggat tgtgctcgcc
     1381 aacgtcacca ggttgtcgag gacgacctca attttgggac gcgagctctt gagtatgtgc
     1441 gtcgtctcct ccagtgcagg cagcaatcgc gctcgcacca tctcccacat cacgtcgacc
     1501 agataggtga cataggtgac tgactccagt cgttcgttaa gcctgtgcag gatgaagcac
     1561 agggccgcca gaaaaaccat ctgtaggacg atagcgactg cgatgacggc cataaagatg
     1621 gtgagcttgt catccatata acttccacgg agcgacggtt taccccaggc ggcggctatt
     1681 ccgttttatc tcgcggctga ccgctactgt acacccggtc cttgctgaaa actatgctcc
     1741 agcccatttc cctgtttttc cgcttgctcc cgcttgggtc cggccagcgt gtccctgaac
     1801 ttccgggaag ccccgagctt cgatgatccg tcgcgatcac acgcaagctg ggataatggt
     1861 ctgccgcaat cttcaatagt tccgagggat tctggaggcg atggatttca tcgagcatga
     1921 tcgatcggtt ccgcaagccg tcaaagaagc cctctggatc cgatcatgcg gcgcacgcgc
     1981 ggcagctcac agtcgaacta ctctatctgg ggcaggtcgg ccaagggtag aaatgtagcc
     2041 cgccattcgg attagacgtc atcagcaggc agcttgtaac gcagcacctc aatttcgtcc
     2101 gcagggtccg gcaaagtgat ttccaatacc tgattaacca gatcgttgtc gcaatcgagt
     2161 tcgtcgagcg cagcttcaaa caattcgaac tgaagatcga gatccatgcc gaccgggcca
     2221 ttgaggcata tcagcccggc gtgcagtgcg acatccgcat attgtcccct ggaaccaggc
     2281 gcgtcgtctg ggccacgaaa atcgatgctg ttcttggtga cgaacgtcca atcgccgtcg
     2341 aggataacgg ccttgagttc ccagtccttc cacccgctct tgccaaccca agcgatatgc
     2401 gaagcttctg catgaccccg ccgctgagcg agcttggtca attcgtcgct taggcattcg
     2461 tcaacaagta gtttcacgac gccagctctc gccgcggttt gcggcgagtg gagacgatga
     2521 ccgctccggc gggcggcgac gcaggctggc gtggtctgcc gcgctgcggg ttggcttcgg
     2581 catagaacga ggcaagctca acagcttcag cggtgagtcc gggatagacg gccaaaatgc
     2641 gagccgtggg cagtcctgcc gcgacagagg ccgcgacatc ataaaccgga atgcgtgtgt
     2701 cccgaataac gggagttccg cccataatat cggcatcttc aacgacaagc gcttgtgctg
     2761 ccgccaatct ggccagtcgc tcgtggacgc ttctcaggaa tggagccaga tcaatcgtca
     2821 gaaactcctc gcggatgatc cattccttct ccaacacgct cccggattcc tcgcgtaaac
     2881 gggcgcttgc aacggcaatc gtgcgcatgc gctcctcaga cgtcagtcga ttggctgctc
     2941 tgaaatagaa cgagatgaaa gcgcaggcat ctgcataaag ccggcgcgcc cgatcacggt
     3001 ctgccacgta gaaggcttcc ggaagaatct tctcgtcaat gattctgttc acatcccgca
     3061 cgggtacaca cgccacaaca gcggcttcgg tgggtgtcaa cgtttgcact tttacggcac
     3121 acatgatgtc tccataatta ttccgacata tcggaatata taatagatgc gatctccatt
     3181 gcaaggactc gctgatcggg gtggctgtgg aaaaacccgg atctgaccct agcggaatta
     3241 cagcaactgg tggcgaaccg gctctgtctc gaccagcgtg atctggttga ggctggcttg
     3301 atccgcaagc cgggagtcga tcttggtctg tttgacgaac ttgtgtacta agtatccgcc
     3361 catgccgccc agaatgaatc gccgtcgcgc ccaggaggtg ctcagcaaga tcgacgcgat
     3421 tctggcctgg gaggcacggc atgagaacga gcgcgatacc aggtttgtgg aactgggcaa
     3481 gtacctttgc gaggtgcgcg ccggccagta ctggcggctg gagaacctga agtcgtttga
     3541 tgagttcctg gagcgccggt tcccggagtc cagaaggaaa gcgtactacc tgatgtcgat
     3601 tcacgaacag cttccacctc aggtgaggaa ggaactgaaa caggtgggat ggacgaaggg
     3661 cctggagttg gccaagctgg cgcggcagca gggtcagcgc ttcgaaagtg caacctggct
     3721 gcacaaagcc cgacagatgc ccaaggcaga gttccagcgg gaggtcgaaa gggaactgac
     3781 aggcaaggac agcgagccct cggagctgat ctatttcaag gtctatcaga gccagctgcc
     3841 ggttatcgag cgggcgatcg aggctgcggc cttgatgctg gggtcggaca agttccgggg
     3901 ttactgcctg gagatgatct gtgcggactt cctctccggg gcctcaatcg acggcggcaa
     3961 tccggagatt ctgctgcagt ccatctcgcg atattacaag tttctgccca gcccacaaca
     4021 ggaggccttt ctgtcggagg tcaactggaa ggcctcatga agaaggtcag ccgtaaaagc
     4081 ccgcgactta gactcgatgc gaacagctac ctcgaactgc atcggcaggt gctggagcgc
     4141 gacggctggc gctgccagtt ctgcgggagc atgcaaaacc tgcaggtcca ccacctcaag
     4201 ttccgcagcc actcgggcgg cgacgaggaa cagaacctga tcacgatgtg cgccgaatgc
     4261 cacgcgcgca tgcattcaaa agctacctgc agaccggata acttcaatgg gcgctgatca
     4321 gaactcgatg cgtgctgaaa gggcggatca gcggcggcag
//
LOCUS       DASRKK010000299         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1097786, whole genome shotgun sequence.
ACCESSION   DASRKK010000299 DASRKK010000000
VERSION     DASRKK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097786"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFC15_06530"
     CDS             <1..313
                     /locus_tag="VFC15_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09851.1"
                     /translation="KPLTQYDAIATPILAFDTQPSNGATFNAIKASQDVMCQKVSRLV
                     LAKGNPMRKWAIESAKMNFDIPDSAPSAKLNEVLWKSVKGPNSKMPKIQHGVITATDK
                     D"
     gene            complement(1402..2445)
                     /locus_tag="VFC15_06535"
     CDS             complement(1402..2445)
                     /locus_tag="VFC15_06535"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM09852.1"
                     /translation="MIVWVRHLLGWVVSALRSREDLILENLALRRQLLALHAKRPRRR
                     LTASHKLFWVVLRRLWDRWKEPLILVTPRTVVGWHRAGFRLYWKWISRARPKGGRKPV
                     SKEVRALIFQTVAENPTWGAPRIHGELLKLGFDLSEPTVSRWIRRAPRPPDPMKHWLT
                     FLRNHREAIAAMDFFTVPTLSFGILYCFFIISHNQRRILHNNVTCNPNALWVALQLHE
                     AWECGEQPERFLIFDRDSKFSVDVVSTVKAMGSQPIRTAFRSPWQNGIAERWVGSVRR
                     ELLDHVIVLNRRHLRRLLNDYVRYYHEDRTHLALEKDTPDGRVPASPAGIGAKVVSLS
                     RLGGLHHRYTVAA"
     gene            complement(2689..4752)
                     /locus_tag="VFC15_06540"
     CDS             complement(2689..4752)
                     /locus_tag="VFC15_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZM09853.1"
                     /translation="MNPWIALRNTSGVTVPIAVGAALGMPLGGLAVGTGALPVSYSDI
                     PGPYAQRARRMVAASVMCSLAVVAGGLAVRRPVFAVILVSVWAFVAGLAVCLGPTAES
                     LGVISLVTLIIYAVQPLTPERALVSGLLALGGGLLQTLLSLLLWPVRHYQPERRALSA
                     LYTELSRASVTPALSGGPPASEQITLARKSLSGLGNDTNLEAERYWSLLNQAERIRLS
                     LLTLRRLRKRMQRDNWDLVGFQSTEEFLTIVADVLASVAKSLSGAESANTARNRLPVL
                     VALAERLRQSETTPSPAFLAALTRDARLQMDALTGQLRSVVRTVNETSPVELEAFVLR
                     EDTQPWSRRFTGILAKLRANLTLRSSAFRHAIRLSLCLVIGGLVAHQLPNRRSYWLAM
                     TIVLVLKPEFATTFSRGALRIGGTILGLLLATGLFHFLPAGMEWKVVLVGLFVFLLRW
                     VGPANYGVFTIAISALIVVLVAFTGVSPKQVIFERGEMTCLGGLIALVTYLVWPTWER
                     TQAAHLLGRMLARYRLYFNSVTEAHLAGQSEDEAELGRLRMSARLERSNLEACAGRLR
                     VEPGSTSEQIDLLTAILANSHRFVRATMALEALPSEAELARKEFQLFASDVNKTLESL
                     ELALLGEKAALRHLPDLREDHRRLVNSSDSELSRHALINEETDRMTNSLNTLTEQVLQ
                     WLRTN"
     gene            5041..5448
                     /locus_tag="VFC15_06545"
                     /pseudo
     CDS             5041..5448
                     /locus_tag="VFC15_06545"
                     /inference="COORDINATES: protein motif:HMM:NF018535.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
BASE COUNT         1342 a         1511 c         1469 g         1126 t
ORIGIN      
        1 taaaccgctc acccagtacg acgccatcgc cactccgatc ctggcgttcg acacccaacc
       61 ttcaaacggc gcaacgttta acgccatcaa ggcctcgcaa gatgtaatgt gtcaaaaagt
      121 gtcgcgattg gtactggcca aaggaaaccc catgcgcaag tgggcaatcg aatcggccaa
      181 gatgaacttc gacatcccgg acagcgcacc ctccgcaaag ctgaacgaag tgctttggaa
      241 gtcggttaag ggaccgaact cgaagatgcc caagatacaa cacggggtta tcacggccac
      301 ggacaaagac taagcagtcg gcgcagttaa acggcctcac aggcaacgat actcgcttcg
      361 aggcagacaa gcagtaacat cgaatagaga cgggatccgg cagagctagc cgggtcccgt
      421 tctgtttggc gcaacgagtg gactgcaccc cgaaagtgag acactcggac actcgcaggg
      481 gaaggactgc gagttgttcg ccaaaactag atcgtgtgct ccgcaaagat ctctgcagtc
      541 gcgcggtgca tgagcatcgt gcaaattcaa gggccgttca ctggccagtg tgcttgaatt
      601 tcgtcaggag gatccaagac tattttgacg agggtaaatc gcggagtgta ggcaaatttg
      661 taactgtcaa agcccccgaa aacttcaaaa tccaacagtt ttcaactagc ggcttgcaac
      721 aggttccata tgaatcactt gcacccaaca gatttccaca agtttcagta acctttggaa
      781 tctggttcgg gaccaggggg tcggaggttc aaatcctctc tccccgacca atcgtttcaa
      841 taagttacag acgctttctc gttctgacga attcccgcgg tgtcgatttc gtgactgtga
      901 aagcttcaca aatccacaac gaaaacgcca tcgcggagag tttgttccca actgtcaccg
      961 aggttcgtcg ttagatgtgc ggaagctgcg gcgaagacgt tgccaagcgc ccaaccttgc
     1021 tctcctcgat acttcgccgt aaattcggtt catgccaaat tcttcttctg aacagttcct
     1081 aaataaggta gagaaaatct tccgagccgt tttcgacaga gtgatcgtct cgactgccgc
     1141 ttcgtatgcc acgctgggga tggtcgagag tcgccgctca agtacactat cgccaatcgc
     1201 agcccttgtg gctcgtcaaa actaagcgcc gcgcttttct tgccaggaaa cttgctttct
     1261 atcaatggaa agaactccgc cagtgaggtc ttcgagtcgc agaatcgtcc tggaaacgag
     1321 tagaagagtt cgactcatgg ggacagaaat ggatctctct tctgccgatg gcgcaggagt
     1381 ctagttcaca gctgcagata gttaggcggc gaccgtgtag cggtgatgca acccaccgag
     1441 tcgggatagg gagacaacct tggcgccaat cccagccggc gacgctggaa cacgaccgtc
     1501 gggagtgtcc ttctcaagtg cgagatgagt ccggtcctcg tggtaataac ggacgtaatc
     1561 attcagcaac cgtctcagat gtcttcgatt cagcacgatc acatgatcca ggagctctcg
     1621 acgtacgctg ccaacccagc gctcggcaat tccattctgc caaggacttc ggaatgcggt
     1681 gcgaatcggt tggctgccca tcgccttcac ggtcgagacg acatcgacac taaacttcga
     1741 gtcgcgatcg aagatcagga atcgctccgg ttgttcgccg cattcccagg cttcgtgcag
     1801 ctgcagcgca acccaaagcg cgttgggatt acaagtcaca ttgttgtgaa ggatgcgacg
     1861 ttggttgtgg ctgataatga aaaagcagta cagaatgccg aaactcaacg tgggtacagt
     1921 gaagaagtcc atcgccgcaa tagcttcgcg atggttccgc aaaaaggtca gccagtgctt
     1981 catggggtct ggaggtctcg gcgctcgccg aatccatcgc gaaactgttg gttccgatag
     2041 atcaaagccg agcttgagca actcaccgtg aatgcgtggc gctccccagg ttgggttctc
     2101 ggcaaccgtt tgaaaaatca gggctcgaac ttctttgctt acaggctttc ggcctccctt
     2161 cggtcgggct cgtgaaatcc acttccagta caaccgaaag ccggcacgat gccagccgac
     2221 cacggttctg ggtgtcacca ggatgagagg ttctttccat cgatcccaga gccttcgcaa
     2281 caccacccag aacagtttgt gggacgcagt gagtcgtcgg cgaggtcgtt tggcatggag
     2341 agcgagcaat tgccgacgca aggcgagatt ctcaaggatg agatcctcgc gggaacggag
     2401 cgcactaaca acccagccga gcaagtgccg gacccatacg atcatggcaa gaatcttagc
     2461 gaaatgacgc caaccgagac gattcccaag tcattgatat tacaggtgca cgatgttttg
     2521 atgaaccata ggtacggcga acgcgccgaa ctgcaacgct tgctggattg cttgtcggag
     2581 cgccgcagca gcgtccgtca gctaatcgaa gacttcagga cttccagcag aaaccccttt
     2641 ccgaattgga acatggcagc ttcaacgccc gcaagatgca ctaccgtgtc agttcgttct
     2701 cagccactgc agaacttgtt cggtgagcgt gtttaagctg tttgtcatgc ggtcggtttc
     2761 ctcgttgatc aacgcatgac gagacaactc cgaatcggac gagttcacca gccgacggtg
     2821 atcttctcga agatcgggca agtgcctaag ggccgccttc tctcccagta gtgccaactc
     2881 cagagactcg agagtcttat ttacatctga ggcgaatagc tgaaactcct tgcgagccaa
     2941 ttcggcttca gaaggtagtg cctcgagcgc catggttgct cgcacaaatc gatgggaatt
     3001 tgccaggatt gcagtcagaa gatcaatttg ctcggaagta cttccgggct cgacccgcaa
     3061 tcgtccggcg cacgcttcca agttggagcg ctcgagccgc gccgacatcc gcaaacgacc
     3121 caattcagct tcatcctctg attgccctgc caagtgcgct tctgtcactg aattgaaata
     3181 caggcgatac cttgccagca ttcgtcccag aaggtgcgca gcctgggtcc gctcccaggt
     3241 gggccagaca aggtaggtca cgagcgcgat cagtcctcca agacacgtca tttcacctcg
     3301 ctcaaaaatg acctgcttag gagaaacgcc ggtgaaggca accagcacga caatcagtgc
     3361 gctgattgcg atggtgaaca ctccataatt cgctgggcca acccagcgca gcagaaacac
     3421 aaaaagtccg accaaaacga ctttccattc catgcctgca gggagaaagt gaaacagacc
     3481 agttgctaag agcaatccta aaatcgttcc gccaatccga agcgcacctc gactgaaggt
     3541 cgttgcgaac tcgggcttca acaccaacac gatcgtcatg gccaaccaat aggagcgacg
     3601 gtttgggagt tggtgagcca ccaatcctcc aatgacaaga cataacgaca aacggatggc
     3661 atggcgaaag gcagatgacc gcagggtaag attggcgcgc aacttagcca gaataccggt
     3721 aaaccggcgt gaccaaggct gcgtatcctc gcggagaaca aatgcttcca gttcgactgg
     3781 agaggtttcg ttcaccgtgc gcacaacgct gcgcaattgg ccggtgaggg catccatctg
     3841 gagtcgagca tctcttgtca aggcagcaag aaatgccggg gatggcgttg tctccgactg
     3901 gcgaagacgt tccgcaagcg ccacaagcac tggcagacga ttacgggccg tgttcgcaga
     3961 ctcagcgcct gacagtgatt ttgctacgct cgcaagaaca tcagccacga tagtcagaaa
     4021 ttcttctgtc gactggaatc cgaccaagtc ccagttgtcg cgttgcatcc gctttcgcaa
     4081 tcgacgcagg gtcaatagac ttagtcgaat gcgctctgcc tgattcaaca atgaccaata
     4141 gcgctcggct tccagattgg tatcgttgcc caagccggac aaactcttgc gtgccagcgt
     4201 gatctgttcg ctcgcagggg ggccacctga cagggccggg gttacagatg cccgtgacag
     4261 ctcggtatac aaagcggaca gggctcgccg ttcgggttgg taatggcgca ccggccaaag
     4321 cagcaacgag agcaaggttt gcagtaatcc accgcccaga gccagaagtc cggaaactaa
     4381 ggctcgttct ggcgtcagcg gctgaaccgc ataaataatg agggtcacca agctgatgac
     4441 acccaaactc tcagccgttg gaccaaggca aacagccaac cccgcaacaa acgcccacac
     4501 gctgactaag atgaccgcga atacgggcct gcgtaccgct aatcctccag ccacaaccgc
     4561 aagggaacac atcacggaag cggcaaccat gcgcctggcg cgttgagcgt acggcccagg
     4621 aatgtcggag taggaaactg gcagtgcacc cgtaccaaca gcaaggcctc ccaaaggcat
     4681 gccaagtgcg gcaccgaccg caatcggaac cgttacgcca gacgtatttc gcaacgcgat
     4741 ccacgggttc actcgcgact tgtcgaattg aaagactttg caccaataag cgttccagtt
     4801 gcttcgcggt tgtttatgag tacggattgg tgcggtagca acttcgttca atggttcatc
     4861 ctttgaccca aatcctgaat gctgcgcgaa tgaattatat cgatattaaa ggggtgtttc
     4921 gtttgaatcg ttctcgaaga agaagcgggt gcgattcgtg actcctcgct tacctgaagt
     4981 cgttgcacat ccaacaatct acttggtccg gatccccatg ggcgctttcc ttgtgcgagg
     5041 cgcggcgagg tgggactgga cgaagcatat gctctggctg ctcgggtccg ggaagttatc
     5101 gaggaaggca cgaacgcagt aaagcgtccg atcatcgcga tcgttgacgt gaagagtcag
     5161 gcttatggcc gacgagaaga aacggctggc atctatctgg cgggtgcggc cgctgcggat
     5221 gcttacggat cagcccgtat ggcaggccac cctgtgatcg cgttagtggt gggccacgca
     5281 ctttccggcg gttttctcac gcatggttac caagcgaacc ggattctggc tttcgacgat
     5341 gacggagtca tgatccatgc catgcacaag caagccgccg cgctgattac ccgccgctcg
     5401 gttaagagcc ttgaagagtt cgcaaagacg gttgctcctt tgtcatac
//
LOCUS       DASRKK010000300         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1292916, whole genome shotgun sequence.
ACCESSION   DASRKK010000300 DASRKK010000000
VERSION     DASRKK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292916"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..857)
                     /locus_tag="VFC15_06550"
     CDS             complement(9..857)
                     /locus_tag="VFC15_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZM09854.1"
                     /translation="MRLGQCKRVIVKVASEVNQNHIFSFAAALSYYFVLAFFPALIAL
                     ATIVGYLPIPDLFNSIVAAMVQVMPPQSTGLVQRVAADVTSRNHAALLSLGLLGSLWT
                     CSSGFATMIEALNVAYNVPETRSGLKTRLLAFELTFLIGILVALAFTFIIVGPHFGSF
                     LAAHLGLSWEFALLWPLLRQVLAAIFIVLAVEGLYFLAPNVKQSFASSLPGAVFSVIG
                     WILLSDGLSLYFQKFAHLNKTYGVLGGGIALLVWLYWSSFVILLGAELNSEILQLVPS
                     LRIEES"
     gene            complement(1241..4018)
                     /locus_tag="VFC15_06555"
     CDS             complement(1241..4018)
                     /locus_tag="VFC15_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09855.1"
                     /translation="MRRPGERKWRIPGGKAAARLREFRMERGLTDIPAGGAMSAYFAA
                     MQQKNQQMAALDPKAPQPRWSPMGPFSIPHGQTYGAAQLAVSGRVSCIAVDPKEPAHI
                     LVGSGSGGLWQSVDDGLSWLPVSAANGEFPMSVGAVAFVPGQSQVIYAGTGEGNAMSD
                     YGVGVWKSTDSGATWSALAGSPFIGLGFYKLAIDPLDVRRMFAATTGGLFQSTDSGAT
                     WEFVKMGPAGQPETRCWDISLHPAVPGDANSTKELFAAAPGGLYQSPDGGSNWNAVPL
                     DTAPNQFRRLAVCHDASRGDVVYAFGQDTYGLPALWRRDEPSMPFYWVFCPRGINLTQ
                     AEYDWFAAVSPDSPNIVYLGAISVWKGELRQDFTWNWVNISSRAQGEGIHPDQHAIAF
                     SPVRPNCIYIGNDGGVFKSPDGGVTWQPLNKGLSITQFEYVTQHPELAAWFLGGTQDN
                     GTLRYEGSEAWYQVALGDGGECATNESSPYTAYHCYYDMSLERSITGGGAGSWTDISP
                     PVKAGYASLFYPPMEVNGNLVVMAGESCWVSTDTGATFVEVYLPADTGIVTAIAIAGP
                     FRFFVGTASGDAYRYDWVGNGWAAPRTLHQPRAGQISSIRADTTRPDRLWISYSDMGG
                     PSIYRSDDGGELWIACRNGLPSAPANIVEMDPANTDIVFAGTDVGVWRSDDAGATWTV
                     FSNGLPNAKVGDLLFHPKARLLRAGTQSRGVWEVDLSGQPEPDPQVYIRRTTVDTGRN
                     PCADGVADPFDAGRIAGWQDSPDILVDAMPYRAASMADMDFVAFEDTRRLAPRPAGGP
                     TRVYVQVHQRGSIPAQNAVVRLFFAVASPGPIPPLPAGFWSQFPSDVLPAGSAWVAIG
                     AAASLATLRTGRPQVAGFQWDVPLALAGKKIWLLALVTASNDKLSTAELDVGVLVPQQ
                     TKCALKKVRTD"
BASE COUNT         1157 a         1587 c         1489 g         1011 t
ORIGIN      
        1 tcgaatggtt atgattcctc gattcgcaga ctaggaacca gttgaaggat ctcggagttt
       61 aattctgctc ccagcagaat gacgaagctc gaccagtaga gccagacgag cagcgctata
      121 ccgccgccga gcacgccata ggttttgttc aggtgcgcaa atttctggaa gtacaggctc
      181 agaccatccg acagcaaaat ccagccgatc accgagaaga cggccccagg caacgatgac
      241 gcgaagctct gttttacgtt gggcgcaagg aaatataggc cttcgacagc gagcacgatg
      301 aaaatggctg ccaagacttg gcgcaatagc ggccagagga gcgcgaactc ccaagagagt
      361 ccgaggtgag ctgctagaaa gctgccgaag tgcggcccaa cgattatgaa cgtaaatgca
      421 agagcgacga gaatgccgat gaggaacgtg agttcgaaag ccagcaggcg cgtcttcaac
      481 cccgaccgcg tctccgggac gttataggcg acattcaagg cttcgatcat ggtagcgaag
      541 ccgctagagc atgtccacaa gctcccaagc agtcctaacg aaagcagcgc ggcgtggttc
      601 cgtgaggtca cgtcagccgc cactctttgt accagacccg tgctttgagg tggcatcact
      661 tgcaccattg cagccacgat gctgttaaag agatcaggaa tgggcaagta accgacgatc
      721 gtggccaacg cgattagggc cggaaagaag gccaatacga agtagtacga cagggccgct
      781 gcgaacgaga atatgtggtt ttggttgacc tcagaagcga ccttgacgat gacccgtttg
      841 cattgcccga gtcgcatgga ttttctctag acttggtctg tgcttgacga tgctatggga
      901 tctgaaaggc cggggtcaag ggtgtccctc gtcaaaacct cgtacaccag tgaacccctc
      961 tgacatgcaa gcctcgcctt ttcaaaccgg tcgagtttct tgaccctacg gggtcccatc
     1021 gggcgaatgc aacgcgatca gaaaccgatc cttctgcgaa cgcggacttg acttgtttcg
     1081 aatgatcacg agcagctctg aaatcctccg gacacgtgga aatcctctgc ggggccgatg
     1141 ttccactcag aaccggtttg gtttcccaga agggcgtcta ttggtactga tacaacaatt
     1201 cggtttcgac aattcccgac ggtcattgga tctagcatcg tcaatcggtt cgcaccttct
     1261 tcagcgcgca tttcgtctgc tgcggcacca aaaccccaac gtccagctca gcagtgctca
     1321 gcttgtcatt ggaggcggtc acaagcgcga gcagccagat tttctttccg gcgagcgcca
     1381 gcggaacgtc ccattggaac cccgccacct gtggccggcc ggtccggaga gtggcgagcg
     1441 atgccgcggc tccgatcgcg acccacgcgg acccggcggg cagaacgtcc gacgggaact
     1501 ggctccagaa cccggccggt agcggaggaa tcggacctgg ggacgccaca gcaaagaaca
     1561 gccgcacgac cgcgttctgc gccggaatgc ttccgcgttg gtgaacctgc acgtacacac
     1621 gcgtcggccc accggccggc cggggagcta gtcggcgggt gtcctcaaag gccacgaaat
     1681 ccatgtccgc catcgatgcc gctcgatacg gcatggcgtc gaccagaatg tcaggcgagt
     1741 cttgccatcc cgcgattctc cccgcatcga agggatccgc cacgccgtct gcgcacggat
     1801 tgcggcccgt atccaccgtg gtgcggcgga tgtagacctg aggatcgggc tccggttgtc
     1861 cgctgagatc gacttcccat acaccacggc tctgcgtacc ggcccgcagc aggcgcgcct
     1921 tgggatggaa cagcagatcg cccaccttcg cgttgggcaa accgttgcta aagaccgtcc
     1981 aagtagcgcc agcgtcgtcc gaacgccata ccccaacatc ggtgcccgca aacacgatgt
     2041 cggtgttggc ggggtccatc tccacgatgt tcgcgggcgc ggaaggcagt ccgttccgac
     2101 atgcaatcca tagctcgccg ccatcgtcgg acctgtagat ggaagggccg cccatgtcgg
     2161 agtacgagat ccacagccgg tccggccgtg tggtgtcggc gcggatcgaa ctgatttgtc
     2221 cggctcgggg ttggtgcaga gttcgcgggg cagcccagcc gttccccacc cagtcatagc
     2281 gataggcgtc gcccgacgcg gtgcccacga aaaagcggaa cggacccgcg attgcgatcg
     2341 cagtgacgat gcccgtatcc gccggaagat agacctcaac aaaagtggcg cccgtatcag
     2401 tggaaaccca acagctttcg ccagccatca cgaccaggtt tccgttcacc tccatcggcg
     2461 ggtagaacag agaagcgtag cctgctttga ccggcggaga aatatcggtc cacgatcccg
     2521 cgcctccacc cgtaatcgat cgctcgaggc tcatatcgta atagcaatgg tacgccgtgt
     2581 agggcgacga ttcgttcgtg gcgcactctc cgccgtctcc tagagccacc tggtaccaag
     2641 cttcgctgcc ctcatagcgc aaggtgccat tgtcctgagt gccgcccaag aaccaggccg
     2701 ccaattcggg atgttgcgtt acatattcga actgcgtgat cgagagacct ttattcaggg
     2761 gctgccaagt cacgcctccg tcaggcgact tgaacacacc gccatcgttg ccgatgtaaa
     2821 tgcaattcgg ccgtaccgga ctgaacgcaa ttgcgtgttg atccggatga ataccctcgc
     2881 cctgcgcgcg ggaggaaatg ttcacccaat tccatgtgaa atcctgcctg agttcgcctt
     2941 tccatactga aatggctccc agatagacaa tattcgggct gtcgggcgac accgcggcga
     3001 accaatcgta ctcggcttga gtcaggttga tgccccgggg acagaagacc cagtaaaatg
     3061 gcatgctggg ctcatcgcgg cgccacaaag cgggcagacc atatgtgtct tggccgaacg
     3121 cgtacacgac gtcgccgcgc gaggcgtcat ggcacaccgc aagacggcga aactgatttg
     3181 gcgccgtgtc cagcggtaca gcattccagt tgcttccgcc gtccggcgat tgatacaagc
     3241 caccgggagc agcggcgaac aactctttgg tggaattcgc gtcgcccggc acggcggggt
     3301 gcaaagatat gtcccagcat cgggtctcgg gttgacccgc cggacccatc ttgacgaact
     3361 cccacgtggc gccgctgtcg gtgctctgga aaagaccgcc agtagtcgcg gcaaacatgc
     3421 gccgtacatc cagcggatcg atcgccagct tgtaaaagcc caagccaata aacggactgc
     3481 ccgcgagagc cgaccaagtt gcgccgctat cggtcgactt ccacactccg acgccgtaat
     3541 cgctcatcgc gttgccctcg cccgtgccag cgtagatgac ctggctctgc cccgggacaa
     3601 acgcaaccgc gcccacggac atgggaaact cgccgttagc ggccgagaca gggagccacg
     3661 aaagaccatc atcgacggac tgccataaac cgcccgatcc cgacccaact agaatatggg
     3721 ccggttcctt cggatccacg gcaatgcaac tgacccgccc tgaaaccgcc aactgcgccg
     3781 ccccgtacgt ttgtccgtgg ggaatcgaga atggtcccat cggcgaccat ctcggttgcg
     3841 gcgcctttgg atcgagcgcg gccatctgct ggttcttctg ctgcatggcc gcgaagtatg
     3901 ccgacatggc gccgccagcg ggaatgtccg tcaacccgcg ctccatacgg aactcgcgca
     3961 aacgagcggc ggccttgccg cccggaattc gccactttct ctcgcctggt ctacgcatgt
     4021 tctgaatccc ctttgcttcg attaagcagc gtgcgtcggc ataacacaag ggcccagact
     4081 gctattccga acaacaagcc gagttccgtg agaccgttcc cgccgtcggg atacaggtgt
     4141 agaagcttct caataaagtc cacgaaccct ccttcgcacc ggagaaatgg ggcggagtgc
     4201 atgctgcacg cagggaggaa aagtcgcccc gaagctgact attctgaatt cggttgtgag
     4261 aactttaagg aatacggcgt tgctggaccc gatacgggga ttatcaacga cttttccatc
     4321 tcacttacga acgagaggta atatattcct ccactccgca acggaatatt aacttttgtc
     4381 ctccccaggc caggtggcaa tcgattcgtt tggtcaggag tgaatggagc acgccactgg
     4441 cttgtcgctt cggaggtctt gtctgaagag cttgaccatc tgcggaagcg ggcggggatg
     4501 atccaaaccg ggcgacttca accttgttct ggacgaagaa ccagggaacc gcagtttcca
     4561 gttcggcgcg gttggcagac aaactgaagc tgtccagtct ggttggggga agttgaccgc
     4621 gccgattccg gtcgacgcgc taaccggaaa caaccaatca gccagcgccc gattctctga
     4681 actcggatgg gaaaggcgct ggaaatcacg cccgaattag caagaatcct gcgtttccag
     4741 acgagcgctg ccggagactt attctgcact tcggactgtc cggtgaggaa gcagtcacac
     4801 tgcgaacaac tttgggagtc aaaccggccc tgccatggcg ccccatcgcc caatgatcgc
     4861 taactcttcg ccttcgagtc gccacagaaa gcggactgct ctgccgcgtg gccgagccca
     4921 atctgtttct ccgctatgct cgtttccata atgaaatccg gttaagtcgc tgacacgggg
     4981 tcttagagca cagtctgaaa ccgtaccaga agttcgaatc cagttccctc cgccagacag
     5041 tctgaactgc agagaaactc tgccgcgttg ctcccttaat tggcgagaaa cgcgggcttt
     5101 tcgcagtatt ccctcaacaa accggacgag agaaaatggc ccggtgagct gcgtccctcc
     5161 cgtttgcagc ggttttctcc agagcctcta ttcgtagtcc ggtttgaagg agtcgttcgg
     5221 gcgaatgatt ggcgatcaca aacc
//
LOCUS       DASRKK010000301         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_413613, whole genome shotgun sequence.
ACCESSION   DASRKK010000301 DASRKK010000000
VERSION     DASRKK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413613"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..482)
                     /locus_tag="VFC15_06560"
     CDS             complement(150..482)
                     /locus_tag="VFC15_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09856.1"
                     /translation="MTPAGAGSPAYVAAVLTLYLDLPDTPLRPSPVDQSLASRLHQQA
                     VPLPLVESALLLATLRRLSRPADLPPLPKIRSLAYFLPVIAELQQQSLPAGYLDYLRL
                     KLRKLAQE"
     gene            complement(479..1270)
                     /gene="istB"
                     /locus_tag="VFC15_06565"
     CDS             complement(479..1270)
                     /gene="istB"
                     /locus_tag="VFC15_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM09857.1"
                     /translation="MNSPAPQTTPTTPSNSLSSQLQQIGLCALPTQLDDFIARATKSR
                     WSAHQILEQLVKAEGAERSRRSLERRLRISGIKSFKPMADFDWSWPAKIERDVIERAL
                     SLDFLGEARNLIMVGRNGLGKTMIAQNLCHTAVLAGHSVLFRSAAALLEDLHRQTPEG
                     RRRKLRTYANVGLLAIDEVGYLSFDDKAADLLYEVVNRRYERKPVILTTNRPFKEWNE
                     VFPNATCIVTLLDRLLHHAEVTVIEGDSYRVRESERETAARRRKK"
     gene            complement(1224..2714)
                     /gene="istA"
                     /locus_tag="VFC15_06570"
     CDS             complement(1224..2714)
                     /gene="istA"
                     /locus_tag="VFC15_06570"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM09858.1"
                     /translation="MISQETRAQIRRYFYAEHWKIGTIARELGVHPDTVRNAIESERF
                     RSMQPLRASLVDPYIAFLRQTLEQHPSLRATRLYQMIRDRGYSGSVVQLRRSVARLRP
                     HSREAFLQLHVFAGEQAQVDWAHFGHVMVGRARRALSCFVATLSYSRALYLEFFFDQT
                     TENFLRGHVHAFQAWSGQPRVILYDNLKSAVLERRGNQILFHPRLIELSAHYHFAPRP
                     CQVRAGNQKGRVERAIRYVRDSFWAGRTFTTLAECNRQALLWRDQVAHQRRWPGGDHR
                     TVAEAFDEEQPRLLPPALHPFSTDRIETVRSHKTIYIRFDLNDYSIPPEAVGRQLTLL
                     ASDTCVRIIDGSVEIARHTRTYDRHQLVLDPAHQEAVLKIKRKAFHSTPGGRLEQAVP
                     ESKILLDLAFAHGESAGNQTAQLTRLLEQYGATALRRAIAEALQRNTPRASSVAFLLR
                     RQERPTLLALDLSGHPLAQSIDVRPHDLETYDELARTPDNTDDPEQ"
     gene            2758..2940
                     /locus_tag="VFC15_06575"
     CDS             2758..2940
                     /locus_tag="VFC15_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09859.1"
                     /translation="MKRPKRPQIRQRSHKPDRVSGQCAEKPALPLDSGGGAAATVGAS
                     SFPHSNDAGETIETEP"
     gene            3372..3533
                     /locus_tag="VFC15_06580"
     CDS             3372..3533
                     /locus_tag="VFC15_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09860.1"
                     /translation="MDDRTDDRAFGADDVAPLGPKFRKLRFQMIADTWHPEVNGDVAV
                     VPAIHNPAV"
     gene            complement(3544..>3849)
                     /locus_tag="VFC15_06585"
     CDS             complement(3544..>3849)
                     /locus_tag="VFC15_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09861.1"
                     /translation="GNGSPYQAQLRILRLNVSVVSNGRMARTMRKKTWTVIAVANDTR
                     HLWSCLQKATDAHDAMRQCAAEMGAVDPSNFQIIGAIRGDHDVIASGEDGGPRSGPR"
BASE COUNT          735 a         1094 c         1267 g          753 t
ORIGIN      
        1 cattctggcc gaggccgacc gggccaaagg ctccggcggt gtcggagcgc tactccgccg
       61 aaggtttgta atcttctctg ttgacgacgt ggcgccgcga gtgttggcga tcacgagaaa
      121 acgtagtttt ttgaacatgc ggccggcggt tactcctggg ccagcttgcg aagtttaagt
      181 cgcaagtagt cgaggtatcc agcgggcaat gactgctgct gtagctcggc aatgaccggc
      241 aggaaatagg caagggaacg aatcttcggg agcggcggga ggtcggcagg cctggagagc
      301 cgccgtagcg tggcgagcag caaggcggac tccaccagcg gcaagggcac cgcctgctgg
      361 tggagtctgc tggcgagcga ctgatccacc gggctgggcc gtagcggcgt atcgggcaga
      421 tccagataca gggtcaggac tgcagcgaca taggccggac ttccggcgcc cgcaggcgtc
      481 atttcttcct ccggcgcgcc gcggtttccc gctcactctc gcgcacgcga tagctgtcgc
      541 cttcgatgac tgtcacctcg gcatgatgca gcaaacgatc cagcagcgtg acgatgcagg
      601 tggcgttggg aaagacctcg ttccattcct tgaatgggcg atttgtggtg aggatcaccg
      661 gcttacgttc gtaacgccgg ttgacgactt cgtagagtag atccgccgcc ttatcgtcaa
      721 acgagagata gccgacctcg tcgatggcca aaagaccgac gttggcgtag gtgcgcagtt
      781 tacggcgacg gccttcgggt gtctgccggt gtaagtcttc gagcaacgcc gcggcggaac
      841 gaaacagcac ggagtgaccg gcgagcacgg cggtgtgaca gaggttctgc gcaatcattg
      901 ttttgcccaa accgttgcgg cccaccatga tgaggttgcg ggcttcgcca agaaaatcga
      961 gcgagagagc gcgctcgatg acgtcgcgct cgatcttagc gggccaggac cagtcgaagt
     1021 cggccatggg cttgaagctc ttgatcccgc tgatacgcag acggcgctcc agactgcgcc
     1081 gcgagcgttc ggcgccttcc gctttcacca gttgttcgag gatctgatga gcgctccagc
     1141 gcgactttgt ggcgcgtgcg atgaagtcat ccagttgcgt gggcagagcg cacaggccga
     1201 tctgctggag ttgagaggag aggctattgc tcggggtcgt cggtgttgtc tggggtgcgg
     1261 gcgagttcat cgtaagtctc caggtcatgg gggcggacgt cgatcgattg ggccagcgga
     1321 tggccgctga ggtcgagggc aagcagtgtg gggcgctcct ggcggcgcag caggaaggcg
     1381 acggaggaag cgcgcggcgt gttgcgctga agagcttcgg cgatggcgcg gcgcagggct
     1441 gtggcgccat attgttccaa caacctggtg agttgagccg tctgattgcc ggccgattcg
     1501 ccgtgcgcga aggccaggtc caacagaatc ttgctttccg gtacggcttg ctccaggcgg
     1561 ccaccgggcg tggagtggaa cgccttgcgc ttgatcttga gcaccgcttc ctgatgggcg
     1621 ggatcgagca cgagttggtg gcggtcgtag gtgcgtgtat ggcgagcgat ctcgacggag
     1681 ccatcgatga tgcgcacgca agtatcggag gccagcaaag tcaactgccg gccgaccgct
     1741 tctggcggga tggaatagtc gttgagatcg aagcgaatgt agatagtttt gtgcgaacgc
     1801 accgtctcga tccggtcggt ggagaacgga tgcagagcgg gaggcagcaa acggggctgc
     1861 tcctcatcga aagcctcggc gactgtgcgg tggtcgccgc caggccagcg ccgttgatgc
     1921 gcgacttgat cgcgccagag cagagcctga cggttgcatt cggccagcgt cgtgaaagtg
     1981 cggcccgccc agaaggaatc gcgcacatag cggatggcgc gttccacgcg tcctttctga
     2041 ttgcccgcgc gcacttggca gggccgtggc gcaaagtgat agtgcgcact gagttcgatc
     2101 aggcgagggt gaaacaggat ctgattgccg cgacgttcca gtacggcact tttgagattg
     2161 tcgtacagga tcactcgcgg ctggccggac caggcttgaa acgcatgaac gtgaccgcgc
     2221 aggaagttct ccgtcgtttg atcgaagaag aactccaggt acaacgcccg cgaataggac
     2281 agcgtcgcca cgaagcagga caaggcgcgt ctggcgcggc ccaccatcac gtggccgaag
     2341 tgtgcccaat cgacttgcgc ttgttcacca gcaaacacat ggagttgaag aaacgcctcg
     2401 cggctgtgcg gacgtaagcg cgccacgcta cggcgcaact gcacgacgct gccgctatag
     2461 ccgcggtcgc gaatcatctg gtagaggcgc gtggcgcgta agctgggatg ttgttccagg
     2521 gtctgacgga ggaatgcgat gtagggatcg acgagggacg cccgcagtgg ttgcatgctg
     2581 cggaagcgtt ccgactcgat ggcgtttcgg acggtatcgg gatggacgcc cagttcacgg
     2641 gcaatcgtgc cgattttcca atgctccgcg taaaagtagc ggcgaatctg cgctctggtt
     2701 tcctggctga tcatcgagat gactcacctt ctgggtggaa tgcgtggaaa aggatcgatg
     2761 aagcgtccga agcgcccgca gatccggcag cgcagccaca agcctgaccg tgtttccggc
     2821 cagtgcgcgg aaaaaccagc gctgccgctg gattcgggcg gtggtgcggc ggctacagtt
     2881 ggtgcatcgt ccttcccaca ctcgaatgat gccggggaaa cgatcgaaac ggaaccctga
     2941 tccgtcacgc gaagttgagt tcgccgccgg cggcaccggt actgtcgctg ccggtcgcga
     3001 tgatcgagcc ggccttccac gctctgttgg tgcagggcgt tggccctacg ccgctgtcgg
     3061 gcgcgatttt gttcgcggca ccggacgctg caatagcgat ggccgcggtc gcagtgcgcg
     3121 cagatgaaaa acaccgcgcg acactcttcg tccacgcaaa ctcgttgtcg gagaaccgct
     3181 tcgttgtgaa tcgccgccat gcgttttcgc aggcgggtgg tagacaacgg cagcggcttc
     3241 gtggcaggtt agaaccagct gagtttggtc gacctcacga aggtcgccga acccggaacc
     3301 ctgtcgctct aacggcaggg ttccttcact ttcccgcctt ccctgacacg ataactccgt
     3361 gcagtcggct tgtggatgat cggacagatg accgcgcctt cggcgccgat gatgtcgctc
     3421 ccctcggccc gaagttcaga aaactacgtt ttcagatgat tgccgacacg tggcaccccg
     3481 aggtgaacgg cgatgtcgcg gtagtgccag ccatccacaa tcctgcagtc taacaacgcg
     3541 cggtcatctg gggccagatc tcggcccgcc gtcttcacct gacgcgatta catcatggtc
     3601 gccacgaatc gcgccaataa tttgaaaatt cgatgggtcc acagccccca tctctgccgc
     3661 acactggcgc atcgcgtcgt gcgcgtcggt cgccttttgc agacaagacc aaaggtgtct
     3721 ggtgtcgttt gccaccgcaa ttactgtcca ggtcttctta cgcatagttc tggccatcct
     3781 tccattggaa actacggaca cattcagccg caagattctt agctgcgcct gatacggaga
     3841 cccgtttcc
//
LOCUS       DASRKK010000302         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_902321, whole genome shotgun sequence.
ACCESSION   DASRKK010000302 DASRKK010000000
VERSION     DASRKK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902321"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFC15_06590"
     CDS             complement(<1..1002)
                     /locus_tag="VFC15_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZM09862.1"
                     /translation="MIVGVPRESFPGERRVALIPAVIPSYTKAGIEVIVEAGAGVEAG
                     YPDTQYIDKGAKIVGTREEAFRADVVVQVLCHGSNDKTGAADLPLLHRDQALIGFLRP
                     LGDIQPVQAIAETGVAAFSVELMPRITRAQSMDALSSMATIAGYKAVLIAADTLPRIF
                     PMLTTAAGTITPSRVLVIGAGVAGLQAIATARRLGAVVSAYDLRPAVKEQVQSLGGRF
                     VELPIEAADAQDAGGYAKAQDESFYQRQRELLGKVVAESDVVITTAVVPGKKSPVLVT
                     KQMVEGMAPGSVIVDLAAERGGNCELTRTGEHVVEHGVTIMGTINLASTVPYHASQMY
                     "
     gene            1514..>2110
                     /locus_tag="VFC15_06595"
     CDS             1514..>2110
                     /locus_tag="VFC15_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZM09863.1"
                     /translation="MVYERTTRNPVECSGIGLHSGAPVHLRILPAPTGTGIVFRRSDL
                     DGFEVEAISRNVAKVSYATSLMKKGVLISTTEHLLSAFIGMGVDNAIVELDNLELPIL
                     DGSALPFVEMLTQAGLRQQRRKRVYLKILRPYEMQEGDKFIGVYPAERYSVSYAINFP
                     HPLIGSERFQVELSNGSYVKQIASARTFGFIEQQRAMLN"
BASE COUNT          436 a          632 c          571 g          471 t
ORIGIN      
        1 gtacatctga ctggcgtgat acggaaccgt gctggccagg ttgatggttc ccatgatggt
       61 cacaccgtgc tcgaccacgt gctcgccagt ccgggtgagc tcgcagttgc ctcctcgttc
      121 cgccgccagg tccacgatga cagagccggg agccattcct tccaccattt gtttcgtcac
      181 cagcaccggc gacttctttc ctggaaccac cgccgtggtg atcacaacgt cgctctcggc
      241 gactaccttt ccgagcaatt cgcgctgacg ctgataaaac gattcgtcct gcgccttcgc
      301 gtagcccccg gcatcctgag cgtcggcagc ctcgattggg agctcgacga atcttcctcc
      361 caggctttgg acttgctcct tgactgcggg gcgtagatcg taagctgaga ccaccgcgcc
      421 caatctgcgg gcggttgcta tggcttgcaa gccagcaacg ccggcgccga tcaccagcac
      481 ccgtgacggc gtaatcgtgc cggctgctgt cgtcaacatg ggaaagatcc gaggcagtgt
      541 gtcagcggca atcagcactg ccttgtaacc agcgatggtt gccatcgagg agagcgcatc
      601 catgctttga gcacgcgtga tgcggggcat tagctcgacc gaaaaagcag cgactcccgt
      661 ctccgcaatc gcctggacag gctggatatc gccgagtggc cggagaaatc cgatcaaagc
      721 ctgatcgcgg tggagtaggg gcaaatccgc cgcgccagtc ttgtcgttcg atccgtgaca
      781 gagtacctgt acaaccacat cggcgcggaa cgcttcctcg cgggtgccga caatctttgc
      841 acccttatca atgtactggg tgtcggggta gccggcttcc acacccgctc ccgcttcgac
      901 tatgacctca atacccgctt tggtatagct ggggatcacc gcaggtatca gcgctacgcg
      961 ccgctccccc ggaaaactct cacggggaac accaacaatc atggagtctc ctccctcttt
     1021 ccgcgcttct ggtatcaaca ccagtcgatg cgttaaatta ttgaaattga cggaactgag
     1081 cattctatct agagtcctgc gaatgtccac cttaacctga tcgaatcgaa ccgaaggggt
     1141 ggatttggcc tacagccgcg attcgaggtg gccccaaatg ctgcgatctc ctgaagatta
     1201 ggcgagcttg aaccaagatt ggaggttcca atggaagatc atcggcggtg ccaccctcga
     1261 cagagtcatc tgggactttc ggcgcaagca ggattctttt cagtaaacgc cggactggcg
     1321 ctggctatcc ccgagcatca tacctctcta atacgtctaa gtcatttaat acacgatggt
     1381 tattgtagta ataagcgggc ttcactcttc gcgtagagtg cttattccga cactggctgt
     1441 tgtagaaata caacagccgg tgccttgctc aagcttgtaa tctgttgtct gcgctgctgt
     1501 tagtctttct ctcttggtct acgaaagaac aactcggaat cccgtcgaat gcagtggcat
     1561 cggcctccat agcggagcgc ctgttcatct gcgcatcctg cctgccccca ccggcaccgg
     1621 gattgtcttt cgtcgctccg acctcgacgg ctttgaagtg gaagccatca gtcgcaatgt
     1681 tgcgaaagtc agttacgcca ccagcttgat gaagaagggc gtgctcattt ccaccacgga
     1741 gcacctgctg tcggcattta tcggtatggg tgtggacaat gccatcgtgg agctcgacaa
     1801 cctcgaactc cccatcctgg atggcagcgc gctaccgttc gtagagatgc tcacccaggc
     1861 gggcttgcgg cagcaacggc gcaaacgcgt gtatctcaag attctgcgtc cttacgagat
     1921 gcaggaaggt gacaagttca tcggcgtata tccggcggaa cggtactccg tcagctacgc
     1981 gattaacttc ccgcatcccc tgattggcag cgaacgattc caagtcgagc tttccaacgg
     2041 cagctacgtg aagcagatcg cttcggcgcg gacctttggc ttcatcgagc agcagcgcgc
     2101 gatgctgaac
//
LOCUS       DASRKK010000303         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1098265, whole genome shotgun sequence.
ACCESSION   DASRKK010000303 DASRKK010000000
VERSION     DASRKK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098265"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..680
                     /gene="folK"
                     /locus_tag="VFC15_06600"
     CDS             189..680
                     /gene="folK"
                     /locus_tag="VFC15_06600"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZM09864.1"
                     /translation="MEKTVYLSLGSNMGDCEANLRTAIERLAELGEVVAVSSFYETEP
                     VEFAVQPWFLNCAVALRTELMPKLFLSKTLAIEQQMGRRRSQPKGPRTIDIDILLFGS
                     SVVHTPQLDVPHVAMHERRFVLEPLAEIAPEVRHPIFKRTVHEMLQALPHNVGQVKKL
                     ARS"
     gene            complement(677..1048)
                     /locus_tag="VFC15_06605"
     CDS             complement(677..1048)
                     /locus_tag="VFC15_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM09865.1"
                     /translation="MEKVNVAQKLSLFTDQFSPKIVGEVNDLYVKLVKLQGEFMWHHH
                     DHEDELFFVIKGALRMKMRENGSERAFMVNPGEFIIIPRGVEHFPSADEETHVMLLEP
                     KSTLNTGNVASERTVHELQRI"
     gene            1132..1410
                     /locus_tag="VFC15_06610"
     CDS             1132..1410
                     /locus_tag="VFC15_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM09866.1"
                     /translation="MMSFRMERDMEEEARQRVNQNGRVVIPAAFRKALGIEVGDEVVL
                     RLQGDELRITTQQRRIQRAQRRARQYIKPGKSLVDELLAERREAAKNE"
     gene            1403..1789
                     /locus_tag="VFC15_06615"
     CDS             1403..1789
                     /locus_tag="VFC15_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM09867.1"
                     /translation="MNRIVLDASAILAVINGEPGHEKLTSELLASAVGSTVNLAEVQA
                     KLVSRGWASDEAWEDTTSPIRETVPFNEEHAKIAGSLVALTRHLGLSLGDCACLALGI
                     ALKAPVYTADKTWKGLKLNVRIHVIR"
BASE COUNT          444 a          590 c          593 g          435 t
ORIGIN      
        1 accgccatcg tctcccgcat tctgcgcgat ccgacctacg cggaagaagt caccctggac
       61 gaaacggcgg ttgccgattg gccggccgag ccggtcgccg ccgccgacta gctgacccgt
      121 tttccgcttg cgccccgcgt accttccgag caactatgca gcagattcaa tcgcgttaac
      181 atacatgcgt ggaaaagacc gtctatcttt cgctgggctc caacatggga gactgcgagg
      241 ccaatcttcg tacggcgatt gagaggctcg ccgagctcgg cgaagtggtt gcagtgtcgt
      301 cgttctacga gaccgagccg gtggagttcg cggtacaacc ttggtttctc aactgtgcgg
      361 tggcgctgcg caccgagttg atgccgaaac tttttctgtc gaaaactctg gccatcgagc
      421 agcagatggg ccgccgtcgc agccagccga aaggtccgcg aaccatcgat atcgacatcc
      481 tgctgttcgg gagttccgtg gtccacacgc cgcaattaga tgtgcctcat gtggcgatgc
      541 acgaacggcg attcgtgcta gaaccgctgg ccgagattgc gcccgaggtg cggcatccca
      601 tcttcaagcg cacggtgcac gaaatgctcc aggcgctccc ccacaatgtg ggacaggtga
      661 agaagctggc ccggtcttag attcgctgca actcgtggac cgtgcgctcg ctggcgacgt
      721 tgccggtgtt gagcgtgctc ttcggctcca ggagcatcac gtgggtttcc tcgtcggcgg
      781 aaggaaaatg ctcgacgccg cgaggaatga tgatgaattc gccaggattc accatgaacg
      841 cgcgctcact gccgttctcg cgcatcttca tgcgcagcgc acctttgatc acgaagaata
      901 gctcgtcttc gtggtcgtga tgatgccaca tgaactcccc ttgcagcttc accagcttca
      961 cgtaaaggtc gttcacttcg ccgacaatct tggggctgaa ctggtcggtg aacagcgaca
     1021 gcttctgcgc tacattgacc ttttccatac gaacccccaa ccagccgagt cttgccctct
     1081 gtcagattct actcgaaccg ggatgaactg ttattgtgac agtatgttag aatgatgtca
     1141 tttagaatgg agcgtgacat ggaagaggaa gcccggcagc gtgtaaacca gaacggtcgc
     1201 gtcgttattc ctgcggcatt ccgcaaggcg cttggcattg aggttggaga tgaagttgtg
     1261 ctgcgtctcc agggagacga attgcggatc accacccagc agcggcgcat tcagcgggca
     1321 cagcggcgcg ctcggcaata cataaaacct ggcaagtcgc tggtcgatga actgcttgcc
     1381 gaacgccgcg aggcagccaa gaatgaatag gatcgtgctg gatgcttccg ccattctcgc
     1441 ggtcatcaac ggggagccag gacacgagaa actaacctct gagctgcttg ccagcgccgt
     1501 cggcagcacg gtcaatcttg ccgaagtaca agccaagctg gtgagtcgcg gatgggcttc
     1561 cgacgaagca tgggaagata ccactagtcc cattcgggaa accgtgcctt tcaacgagga
     1621 acatgccaag atcgccggca gcctggttgc tctgacgcgg caccttggcc tctcgctagg
     1681 agactgcgcg tgcctcgcgc tgggaatagc gctcaaggcc cctgtctaca ccgccgacaa
     1741 aacatggaaa gggctcaaat tgaacgtccg catccacgtc atccgctagg ctggtacggt
     1801 ggtttctgca gaagcctgga tctcactata cctttatccg agctcgaatc tcctgtgtct
     1861 ctccatacgt gaaagacgga ttgtcctcct cttgacctgg attattcatg aggacttcac
     1921 gctttgaagg ggcacggctt cagaggctgc ggaaaaagtc gctggtacct gggaaggcac
     1981 gagcctttcc acaggcaaat aggttatgta agtagcatgc gtggagatga gaagcagcaa
     2041 gacgcgatgt tcagctacat ca
//
LOCUS       DASRKK010000304         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_23240, whole genome shotgun sequence.
ACCESSION   DASRKK010000304 DASRKK010000000
VERSION     DASRKK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23240"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFC15_06620"
     CDS             complement(<1..192)
                     /locus_tag="VFC15_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09868.1"
                     /translation="MATVDAKTSQQALNTILAAIGMRESDYKGKVTIQGKDPILASRH
                     RFGELMAASQAAFGMALSEL"
     gene            complement(1169..1480)
                     /locus_tag="VFC15_06625"
     CDS             complement(1169..1480)
                     /locus_tag="VFC15_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM09869.1"
                     /translation="MLWQGVHLRFSWDERKNRINRRKHRVSFETAALVFDDPCHISHL
                     DREVEGELRWQTIGMVKGIHVLLVVHTSLESDDEEGENVRIISARKATPQERWVYAQG
                     E"
     gene            complement(1518..2255)
                     /gene="rph"
                     /locus_tag="VFC15_06630"
     CDS             complement(1518..2255)
                     /gene="rph"
                     /locus_tag="VFC15_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZM09870.1"
                     /translation="MVYRSDNRSPEQMRPVNIVPDFISTAEGSCLIELGNTRVICTAT
                     IEDTVPQFLRNRGKGWVTSEYGMLPRSTLTRTPREANKGRQSGRTHEIQRLIGRSLRA
                     VTNLDKLGERTIFLDCDVIQADGGTRTASITGAFIAMGLAMRRMIESGTLTVAPIRDY
                     IAATSVGIVDGEIMLDLCYEEDARADVDMNLVITGSKKIVEVQATAEHRPFDDAQLAK
                     MMSLARSGIESLIVRQQAVLTGLPLRQ"
     gene            complement(2471..3469)
                     /gene="murI"
                     /locus_tag="VFC15_06635"
     CDS             complement(2471..3469)
                     /gene="murI"
                     /locus_tag="VFC15_06635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZM09871.1"
                     /translation="MTLPRIGVFDSGVGGLTVLKAMAEQLPAHYLYFGDTARLPYGTK
                     SAETVARYAIGAARFLEAQNIDLLVIACNTATALAFPLIQEAAHVPVVGVVEPGAAMA
                     AEVSKSRSAIVIATEATVSSHAYRDALQRHGLAAHEKACPLFVPLVEEGWIEHPVTEQ
                     VARIYLGEALQQAPERADVLVLACTHYPLIAPLLRRIAPEHMQIVDSAESTARVVKKI
                     IDWHPTLSPTAGDQGGAPAQELENPAVILSDSGNNQAARGRDESANQSVILSEVRLPL
                     AAAERSRRTPIDAKIPPAEHRTFRFYVTDSTDKFKRLATRFLGRQVDNVEHVDLGG"
BASE COUNT          679 a         1017 c         1078 g          757 t
ORIGIN      
        1 tagttcactg agggccatac cgaatgcggc ttgactggcg gccatgagtt cgccaaaacg
       61 gtgccgcgag gccagaatgg ggtccttacc ttgaatcgtt accttacctt tgtagtctga
      121 ctcgcgcatc ccgatcgctg caagaatcgt attcagggct tgctgagaag tttttgcgtc
      181 aacggtggcc atgctttcct tcctccagag atttgggcgc gactaagtat agccgattga
      241 accaggcgtt tgacatgcac gaaggcaacc tctcgggacg gttaccagcc gggtcgccga
      301 agacaacagg ccttagggcg ggtggggcac ccacgtttgg tatttctact gctggggcac
      361 taggctacga ttcgtaagga atcgctcaag agcacaggcc gctcggggat gggctgcgat
      421 ggtggagaca tcgagcccca gccgttgggg ttgttgtaga agctccgggc ggcggcagca
      481 gcagtcagaa gcgggcgccc gaatccctgt gtgggagggc gctgccggaa cgtggcccgg
      541 accatcacca cacaccgctt cggattgtgg gcggttgacg cgcaatccgg aagttgagtg
      601 tcagttgacg ataacagccc tcatcacaac caatcaggct agacagcttg ctaaagaggt
      661 cggcaaacat cactgcctga gcggcaagcc cgtcagcacc gactgctggc gcacgatcag
      721 cgattcgatg ccactccttg ccagcgacat catcttggcc aactgggcat catcgaaggg
      781 acgatgttca gcctggtgcc tggtgcccca ggttagcgtc cctcttttgg acgctaacct
      841 gggttgagga aggtccgggc gcgggccgac tcagggcttt cccatgtccg caagcatccg
      901 ctgacgaagg aggtgattcg ctcgggtctg atatcccttg ccgggttttt ttagccaagc
      961 taccacgtcg gcatcgagac gcagcgagac ggcttgcttc actggacgat agaacctgcc
     1021 ccgtactgcg tccttccagg cgcggagagg taattccgga atgtcggatg tgtcgattcg
     1081 gtcatctggc agggcagcca aggccgctag gtcccgcttc tgtttcgtgg tcagcggctg
     1141 cctgcttcca gcactgctgc cggccggcct actcgccctg cgcatagacc caccgctcct
     1201 gcggggttgc ttttcgcgct gagatgatgc ggacattctc tccttcttca tcgtcagact
     1261 ccagacttgt gtgtacgacc agaagcacgt gaattccttt taccatgccg atcgtttgcc
     1321 atctcagttc tccctcgacc tcccggtcga ggtgagagat atggcaggga tcgtcgaaaa
     1381 ccaaggctgc ggtctcaaaa ctgacgcggt gctttcgcct gttgattcga ttctttcgtt
     1441 cgtcccaact gaatcgcaag tggacccctt gccataacaa ttatgtatat acatcgagtg
     1501 aaagtccaga cccataatca ctgtctcagc ggcaagccgg tcagcaccgc ttgctgccgc
     1561 acgatcagcg actcgatgcc actccttgct agcgacatca ttttggccag ttgggcgtcg
     1621 tcgaagggac ggtgttcggc cgtggcctgc acttcgacaa tctttttgct gccggtgatc
     1681 accaggttca tgtccacgtc ggcgcgcgcg tcttcttcgt agcaaagatc gagcatgatc
     1741 tcgccatcca cgatgccgac gctggtggct gcaatgtagt cgcggatcgg cgcgaccgtc
     1801 agcgtacccg actcaatcat gcggcgcatg gccagcccca tcgcgatgaa agcgccggtg
     1861 atggacgccg tccgcgtgcc gccgtcggcc tgaatgacgt cacagtcgag gaaaatggtg
     1921 cgctcgccca acttatccag attggtcaca gcgcgtaagg agcgaccaat gagccgttgg
     1981 atctcgtggg tgcgcccgct ctggcgtcct ttgttggcct cgcgcggcgt gcgggtcagc
     2041 gtggagcgtg gcagcatgcc gtattcgctg gttacccagc ctttgccccg gttgcgcaga
     2101 aattgcggca ccgtgtcttc aatggtcgca gtgcagataa cgcgagtgtt gccgagttcg
     2161 atcaggcagg agccttcggc ggtggagatg aagtcgggga cgatgttgac cggccgcatc
     2221 tgctcaggag aacgattatc ggagcgatag accatacgcg gcgattgtat agcttcggac
     2281 ggccgctttt cgcctttcgc tgttcgctgt tcgctattcg ctattcgctt ttcgctaaca
     2341 aagtggggac gacaggtgcg gcattggccg aacagcgcag ttaggacaaa cggcgcagta
     2401 ggacaaaaca gggtgttgct tccaactcga agttgacttg cgaaaagcga agagcgaaca
     2461 gcgggttctc tcacccgccc aagtccacat gctcgacgtt gtccacctgg cggccaagga
     2521 agcgcgttgc tagacgtttg aatttgtcgg tggaatcggt gacatagaag cggaaggtgc
     2581 gatgctcggc gggagggatt ttcgcgtcaa taggggtcct tcgactccgt tcggctgccg
     2641 caagcggcag cctcacttcg ctcaggatga cactctggtt agcactctcg tctctcccgc
     2701 gggcagcctg attattaccg ctgtcgctca ggatgacagc aggattttca agctcttgcg
     2761 cgggtgcccc accctggtcg cccgcggttg gtgacagggt gggatgccaa tcgataatct
     2821 tcttgaccac gcgcgcggtc gattcagcgg agtccacaat ctgcatgtgc tcgggcgcga
     2881 tgcggcgcag cagaggggcg atgagcggat agtgggtgca ggcgagcacc agaacatctg
     2941 cccgctcggg cgcttgctgc agcgcttcgc cgagatagat cctggcgacc tgctcggtca
     3001 cgggatgctc gatccatcct tcttcaacca acggcacgaa aagcggacaa gctttttcgt
     3061 gggctgccag gccatgtcgc tgcagcgcat cgcggtaagc gtggctgctc acggtggctt
     3121 cggtggcgat cacaatggcc gagcgactct tgcttacttc ggcggccatg gccgcgccgg
     3181 gttcgaccac tcccacgaca ggaacatgtg cggcttcctg gatgagcgga aatgcaaggg
     3241 cagtcgcggt gttgcaggct atcaccagca gatcgatgtt ctgcgcttcg aggaagcggg
     3301 cggctccgat tgcgtaacgc gcgacagttt ccgccgactt ggtgccataa ggtaggcgag
     3361 ccgtgtcgcc gaagtacagg taatgcgcgg gcaactgctc ggccatggcc ttcagcacgg
     3421 tgaggccgcc gacgcccgag tcgaaaacgc caatgcgcgg cagggtcact gcaaatcctt
     3481 tacgacctgg ccaatattgc tggcttcata aaatcgacgc aagtcagcat g
//
LOCUS       DASRKK010000305         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_217997, whole genome shotgun sequence.
ACCESSION   DASRKK010000305 DASRKK010000000
VERSION     DASRKK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217997"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..3175
                     /gene="infB"
                     /locus_tag="VFC15_06640"
     CDS             119..3175
                     /gene="infB"
                     /locus_tag="VFC15_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZM09872.1"
                     /translation="MQKIRINDLARELEVKSKAILDALIKVGVTEKKTHSSSIEVDEA
                     ERVKKYFAGHAEPGTSRASRAPADEFKPKLDLSHISKPGDVLKAVTQRAAQPFAVARP
                     ATPPTHATAVGPRQTVAPPSAVRSAPSVAKPVTSVASATPAAPAEPPKQAPRFITPQS
                     VARPPAAIVIPPKPPVAAPPATPPAHLPSATGTALPSEPVIEDQITALEESEVLPSTS
                     EAIESEIPTAVLREVPPALLEAQPATENRAVTGRPVAPGQSAQGIGRPGLPTRPGQPG
                     IGAIAAHAPRRLIVPQTGPRPVYLAPPVAPPRPGAPIAPRGGMPERGKPIFQRPRPGV
                     PPQGQTRPPVRPGERRGPHPTRSGAPGARPGFGAGAGAAPPPPTGQRPAGRPARPGQR
                     YVPRGVKEGPMKGFVPPPRLSLSSEPLPITRSITIPEGISVKDLAEKLGIRAKDLIAR
                     LLARGVFATVNQTLDSDLATDMARHFGAETAVITFEEQLAKDTVPAEGAGEEGAALGV
                     ARPPVVTIMGHVDHGKTSLLDAIRSTNVAGGEAGGITQHIGAYKVAITDPNSPALGRE
                     IVFLDTPGHEAFTRMRARGSKVTDIVVLVVAADDGVMPQTLEAIDHAKAANVPMIVAV
                     NKIDKPDALPERVKKQLADRGLMPEDWGGSTVFVDVSAKQKTNLNLLMEMICLVADLQ
                     DLKAVPDRLAQGTVLEAKLDRGRGPVATVLVQNGTLKTGDNFVVGNVFGRVRAMFNDR
                     GVAMDAATPATPVEILGLESLPQAGDQLVAVADREKARGISEYREGKAREAQLAKSSR
                     VSLEGLAEQLKSAGQKELDIILKGDVQGSVEVLGDVLTKLSNDRVKIKILLSGVGAIT
                     ETDVLLASASNAVIIGFNVRPERKAQELAEQEKVDIRLHSIIYELADEIKRAMTGLLE
                     PIIKETYLGRADVRDTFRVPKIGTIAGCYVTDGVIKRDAEVRLLRDNVVVFKGKIGSV
                     RRFKEDVSEVRHGMECGISIANYGDIKVGDVIEDFVIERVAAEAFV"
BASE COUNT          706 a         1058 c         1108 g          620 t
ORIGIN      
        1 ctggcgagca gaagtgagga ctaccgccct cagttgctgc tgtgggccgg gaaaatttcg
       61 cagtgaggat gctatgaagg cttaatcggg cgggacaaca cggaggcaat agggcggaat
      121 gcagaagatt cgaattaacg acctggcaag ggagctcgag gtaaagagca aggcgatcct
      181 cgacgctctc atcaaggtcg gtgtaactga aaagaaaacg cactccagtt ccatcgaagt
      241 ggacgaagcg gagcgcgtta agaagtactt tgcagggcac gcagagccgg gaaccagtcg
      301 cgcctcgcgt gcaccggcag acgaattcaa gcccaagctt gacctgtcgc acatctccaa
      361 acctggcgat gtgctgaagg cggttacgca acgggccgcc cagcctttcg cggttgcacg
      421 tccggccacg cctccgaccc atgctacagc cgtcgggccg cggcaaaccg tcgctccgcc
      481 ttcagccgtt cggtcggcgc cttcggttgc gaaacctgtg acatcggtgg cgagcgccac
      541 tccagcagct cctgcggaac cgcccaagca ggcgcctcgc ttcattacgc ctcagtcggt
      601 cgcgcgccca cccgccgcca tcgtcatccc accaaaaccg ccggtagcag cgcctccggc
      661 tacgccgcca gcgcatctgc catccgcgac tgggacagcg ctaccttccg agccagtgat
      721 cgaggaccag ataacggcac tcgaagaaag tgaagttttg ccgtctacgt ccgaagcgat
      781 cgaatccgag attcccactg ccgtactgcg cgaggttccg ccagcgctat tggaagcaca
      841 accagccacg gaaaatcgtg cagttaccgg ccgtccggtt gcgcctgggc agtctgctca
      901 aggtatcggc cgtcctggcc tgccaacgcg tcctggacaa ccgggaatcg gggctatcgc
      961 cgcgcacgca ccacgccgtt taatcgttcc ccagaccgga ccgcgtccgg tgtatctagc
     1021 tccacccgtg gctccgccgc gccccggcgc tcctattgcg ccgcgaggcg gtatgccgga
     1081 acgtggcaag cctatcttcc agcgtccgcg tccgggggtg ccgccgcaag gacagacgcg
     1141 gccaccggtg cgtccgggtg agcgccgcgg tccgcatcca acgcgttccg gggcgccagg
     1201 ggcccgtcca ggcttcggag ctggcgcagg cgccgccccg ccgcctccta cgggacagcg
     1261 ccccgccggt cgcccggcac gtccaggaca gcgctatgtt cctcgaggcg tgaaggaagg
     1321 cccgatgaag ggctttgttc cgccgccgcg gctttcgctt tccagcgagc cgctgcccat
     1381 cacgcggagc atcaccattc ctgaaggcat cagcgtgaaa gacctggcgg agaaattggg
     1441 aattcgagcc aaggacctta tcgctcgctt gctggcgcga ggcgttttcg ccaccgtcaa
     1501 ccagacgctc gattccgacc tggcaaccga tatggctcgt cacttcggag ccgagacggc
     1561 cgttatcacc ttcgaagagc agttggccaa agacacggtg ccggcggaag gcgctggcga
     1621 agaaggtgca gccctggggg ttgcgcgtcc gccggtggtt accatcatgg gccacgtgga
     1681 ccacggcaaa accagcttgc ttgacgctat ccgctcgacc aatgtggccg gaggcgaggc
     1741 gggtggaatt acgcagcaca tcggcgccta caaggtggca atcaccgatc ccaactcgcc
     1801 ggctcttggc cgcgagattg tcttcctcga cacgcctggc cacgaagctt tcacccgcat
     1861 gcgtgcgcgt ggatcgaagg tgactgacat cgtggtgctg gtggtggccg ccgatgacgg
     1921 tgtgatgccg cagactttgg aagccatcga tcacgcgaaa gccgcgaacg tcccaatgat
     1981 cgtcgcggtg aacaagatcg acaagccgga cgctctgcct gagcgggtga agaagcaact
     2041 cgccgatcgt ggcctgatgc cggaagactg gggcggcagc accgtcttcg tggatgtctc
     2101 ggcgaaacaa aagaccaatc tcaatctgct gatggaaatg atctgcctgg ttgccgacct
     2161 gcaggacctg aaggcggtgc ctgaccgtct ggcgcagggc acggtactcg aggccaagct
     2221 cgaccgcggt cgcggtccgg tggctacggt gctggtgcag aacggtactc tgaaaacggg
     2281 cgataacttc gtggtaggga atgtcttcgg aagagttcgc gccatgttca acgatcgcgg
     2341 cgtggccatg gatgccgcga ctcccgccac cccggtggag attctcggcc tggaaagttt
     2401 gccgcaagcc ggcgatcaac tggtggctgt ggcggaccgc gaaaaggcgc gcggcatctc
     2461 cgaatatcgc gagggcaagg cgcgcgaagc tcaacttgcc aagagttcgc gagtctctct
     2521 cgaaggcttg gcggaacaac tgaagtccgc aggccaaaaa gagctggaca tcatcctcaa
     2581 gggcgacgtc caaggctcgg tggaagtgtt gggcgacgtg ctgacgaagc tgtcaaacga
     2641 cagggtcaag atcaagatcc tgctcagcgg tgtgggcgcc attaccgaga ccgatgtgct
     2701 gctcgcctcg gcgtcgaatg ccgtcatcat cggcttcaac gtgcggcccg agcgcaaggc
     2761 gcaggaactg gcggagcagg agaaggtcga tattcgtctg cactccatca tctacgagtt
     2821 ggcggacgaa attaagcgcg ccatgaccgg cctgctcgag ccgatcatca aggaaactta
     2881 tcttggacgc gcggatgtcc gcgacacctt ccgcgtaccc aagatcggta cgattgccgg
     2941 ttgttacgtc accgatggcg tcatcaaacg cgatgcggaa gttcgcctgt tgcgcgacaa
     3001 cgtggtcgtg ttcaagggca agattggctc cgtgcggcgc tttaaggaag atgtcagcga
     3061 agtccgccac ggcatggaat gcggaatctc gatcgcgaac tatggcgaca tcaaagtggg
     3121 cgatgtcatc gaggacttcg tcatcgagcg agtggcggct gaagcgttcg tttagaaacg
     3181 caggggctag gggtcagggg ctagggccta gagcagattc tgggtgggtg tatccgggtg
     3241 gagcactgct tcagcggtgc atatgagcgt tgcgtctgaa ttcggctaga gcctcgctgt
     3301 ttcttgcagg cgtgcatacc caatacggat actgcaactc tggaattgct cgtgaggtca
     3361 cgcatgcggg taaacggcag ctcccggtac ccggccgaga agtagcatga acctaacgac
     3421 gtcccggaac tgaatcctcc gactcggggg cacggtatcc gggacgcttt gcaatagctt
     3481 gcacttggtg tc
//
LOCUS       DASRKK010000306         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1196114, whole genome shotgun sequence.
ACCESSION   DASRKK010000306 DASRKK010000000
VERSION     DASRKK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196114"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFC15_06645"
     CDS             <1..264
                     /locus_tag="VFC15_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09873.1"
                     /translation="LDIMNDALPGLQRSGTRYTLEPDRRKAIGLAIHEAEGGDIVLIA
                     GKGHEKVQVTVAGAIPFDDIETARNALRSIGYATAQPMQSAKS"
     gene            261..1643
                     /gene="murF"
                     /locus_tag="VFC15_06650"
     CDS             261..1643
                     /gene="murF"
                     /locus_tag="VFC15_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZM09874.1"
                     /translation="MKLSLSRAAEFMQATGEFAPAAIASGYSIDSRTLLPGDLFFAVR
                     GERLDGHDFVEAALVKGAIGVVIAKDQRTRFAQKNQLLAVKDPLLALQRLAAAVRRLW
                     GKPLIAVTGSAGKTTTKEAIAHVLGTKHRVLKSQGNLNNHFGMPLQLLKLEPEHEIAV
                     VEMGMSHAGEITGLAKLAQPDCGVVTMVAPVHLEFFESISAIARAKYELIQSLHAGGI
                     AVLNADDEYVSQFGRDFHGKVITFGLHKPADVSARKIESRGPLGSAFEIVADGEQVPA
                     ALPLLGEHSIYNALAAVAVGLQYGVPLRLAAESIATIAPGDKRGEILNYAGATIINDC
                     YNSNPKALDYMVRSLAQIPARRRLVVAGEMLELGPAAEVMHRDCGQHMASYGIDRLLG
                     VRGLAKSMVEAAAGAGLSAEFVNTPQEAADWLRREVKTGDVVLLKASRGVRLERALEA
                     WVGKTPEPTH"
     gene            1885..>2949
                     /gene="mraY"
                     /locus_tag="VFC15_06655"
     CDS             1885..>2949
                     /gene="mraY"
                     /locus_tag="VFC15_06655"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZM09875.1"
                     /translation="MLYWLLYQKGFLVFHVGPFRIFRYLTFRTVFASLTALFIGLIIG
                     PAVVRRLREFQIGQYIREDGPKDHLKKAGTPTMGGLLIVVGMIVPTLLWADLSNRFVW
                     IAVLATLSFGAIGFTDDYLKVVHRRNLGLTARAKLLYQFLAAFVIGAILSALETHGLY
                     STRLIVPFLKHFRPDLVIGRLHHGYLSPLAFLPFLAFVAIIIVGSSNAVNLTDGLDGL
                     AIGCTVIAAGALTVLTYVSGHAVFADYLELSHMPQVSEVTIFCGSMVGSAIGFLWYNA
                     HPAEIFMGDVGSLALGGAIGTVAVIIKQELLLPFIGGIFVIEALSVILQVGSYKLRRK
                     RIFKMAPIHHHFELMGWSESK"
BASE COUNT          553 a          835 c          925 g          636 t
ORIGIN      
        1 ctcgacatca tgaacgacgc gctaccaggg ttacagcgtt ccggcactcg ttacaccttg
       61 gaacctgatc gccgcaaagc cattggcttg gccattcatg aggccgaggg cggtgacatc
      121 gttctcatcg ccggaaaggg ccacgagaag gttcaagtca cggtcgcggg cgccattccg
      181 ttcgacgata tcgagacggc gcggaatgca ttgcggagca tcggttacgc aaccgctcag
      241 ccaatgcagt cagcaaagtc atgaagctaa gcctatcgcg cgctgccgaa ttcatgcagg
      301 ccactggcga gttcgcccca gccgcgatcg ccagcggcta ttccatcgat tcgcgcacct
      361 tgctgccggg cgatctgttc ttcgcggttc gcggcgagcg gcttgacggg catgattttg
      421 tcgaggcggc actcgtcaag ggcgcaatcg gcgttgtcat tgccaaggac cagcgcacgc
      481 ggttcgcgca aaagaaccag cttctggccg tgaaggaccc gcttctcgca ttgcaaaggc
      541 tcgccgccgc cgtccgccgg ttgtggggaa aaccgttgat cgcggtgacc gggtcggcgg
      601 gcaagacgac aaccaaggaa gcgattgcac atgtcctggg cacaaagcat cgggtgctga
      661 aatcgcaggg caatttgaat aatcatttcg gcatgccgct gcaattgctc aaactggagc
      721 cggagcatga aattgcggtg gtcgagatgg ggatgtcgca cgccggcgag atcaccggcc
      781 tcgcgaagct agcgcagccg gattgtggtg tagtgaccat ggtcgctccg gtgcacttgg
      841 agttttttga gtcgatctca gccatcgcgc gcgccaagta cgagctgata cagtctcttc
      901 acgccggtgg catcgctgtg ttgaacgctg acgatgagta cgtttcgcag ttcgggcgag
      961 acttccacgg aaaggtcatc accttcggac tgcacaagcc ggctgacgtg tcggcgcgga
     1021 agattgaatc gcgcggcccg ttgggctcgg cgttcgaaat cgtggcggat ggcgagcagg
     1081 ttccggcggc cctgccattg ttgggcgagc atagtatcta taatgcgctc gcggcagtcg
     1141 ccgtgggatt gcagtatggg gtgccgctgc ggctggctgc cgagtcaatt gcgaccatcg
     1201 cgcccggaga caagcgggga gaaatcctga actacgcggg cgcgactatt attaacgatt
     1261 gctacaattc caatccaaag gcgctcgact acatggtccg ctcgctggcc cagattcccg
     1321 cgcggcgccg gcttgtagtc gcgggcgaga tgctggaact cggtcccgcg gctgaggtca
     1381 tgcatcgcga ttgcggacag cacatggcga gctatggaat cgacaggctg ctgggggttc
     1441 gaggactggc gaagtcgatg gtggaagccg cagccggggc ggggttgagc gcggagttcg
     1501 tcaacacgcc gcaagaggcc gccgattggc tgcggcgcga agtcaaaacc ggcgatgtag
     1561 ttttgctcaa ggcgtcacgc ggagtgcgcc tggagcgtgc gcttgaggct tgggttggta
     1621 aaacgccgga gcctacacat taaggcagtt tcacacttgg cgtactaggg tggagcaccg
     1681 cttcagcggt gcaggtaaga ttcggaaatg agccggcttt agccgctgag gtgacggaga
     1741 cattacctca ggcgctaaag cgcgatccag gaactgacgg cagttgtgca cgcctgaagg
     1801 cgtgctccac ccatcaccta aacgccagag gtggaactgc tctagggttg cagctcccgc
     1861 ataacgttca gaacggggaa gaatttgctc tattggttgc tgtaccagaa aggttttctc
     1921 gtctttcacg tcggcccgtt ccgaatcttc cgctatttga ctttccgtac agtgttcgcc
     1981 agcctgaccg cgctgttcat tggccttatt attggcccgg cggtggtccg ccgcctgcgt
     2041 gaattccaga ttggccagta catccgcgaa gacgggccca aggaccacct gaagaaagcg
     2101 ggcaccccta ccatgggcgg cttgctcatt gtggtgggca tgatcgtccc aacgttgttg
     2161 tgggccgatc tcagtaaccg cttcgtgtgg attgccgtat tggcgacgct gtcgttcggg
     2221 gcgatcggct tcaccgacga ctatttgaag gtggtgcacc gccgcaacct aggtcttacc
     2281 gcgcgggcca agctgctcta ccagtttcta gcggcctttg tgataggcgc gatcttgagt
     2341 gctctggaaa cccatgggct gtactccact cgtctgattg tgcccttcct caagcatttc
     2401 cggccagacc tggtgatcgg ccgattgcac cacggctatc tttcgccact ggcgtttctg
     2461 ccgttcctcg cgttcgtggc catcatcatt gtgggctcca gcaacgccgt gaatctcacc
     2521 gatggtctcg atggattggc gattggctgc actgtaattg ctgctggagc gctcacggtg
     2581 ctcacctacg tcagcggcca cgccgtgttt gccgattacc tcgagctttc ccatatgccc
     2641 caggtctcgg aggtgaccat cttctgcggg tcgatggtcg gctccgccat cgggtttctc
     2701 tggtacaacg cacatcccgc ggagatcttc atgggcgacg tggggtcgct agcgctgggc
     2761 ggcgcgattg ggacagtggc cgttattatc aaacaggaac tgctgctgcc gttcatcggc
     2821 ggcatcttcg tgattgaggc gctctcggtc attcttcagg tgggttccta caagctgcgc
     2881 aggaagcgca ttttcaagat ggcgcccatc caccaccact ttgagctgat gggctggagc
     2941 gagtcgaag
//
LOCUS       DASRKK010000307         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1392000, whole genome shotgun sequence.
ACCESSION   DASRKK010000307 DASRKK010000000
VERSION     DASRKK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392000"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1144)
                     /locus_tag="VFC15_06660"
     CDS             complement(131..1144)
                     /locus_tag="VFC15_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM09876.1"
                     /translation="MKIASPNKLAFRLRHYFTDHLPRVRGTSVHTVHSYRDSLLLFLR
                     FLASQRNCRVHELDLGDIEPDQVIAFLAHLEEVRNNGVTTRNVRLAAIHAFFRYLGTY
                     HPEHLDRVQRILGIPFKRARQRVIEYLERAEIDVILSRINRANRDGRRDYALLATMFN
                     TGARVQEIVDLRARDLQLIRPFQIRLFGKGRKERFCPLWSQTAHVLRDHCEERHMDLR
                     SDSRVFLNHREEPLTRFGVRYILAKHFDRVRDDVPTLTRKRLHPHSMRHSTAVALLTS
                     GVEITAISQWLGHSSPNTTNRYATIDLEMKRRAIAKVKPVAYRRNPAWRRDQSVLKWL
                     EDL"
     gene            complement(1141..2145)
                     /locus_tag="VFC15_06665"
     CDS             complement(1141..2145)
                     /locus_tag="VFC15_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM09877.1"
                     /translation="MKPTFVFKSALRSTITRYLELNFALGRRYKNTITALKSLDRFLS
                     GFPRASQSLTAEVFDFWCQSQSALTPRVRRYRMVIVRKFCLYRRRTVPDCFVPDPTLF
                     PTPGQTVTPYIFSEAEIARLMRAASSLQRSPYSPLRPEVVRLAVVLLYTTGLRIGELL
                     QLVVGDIDSRGGTLLVRASKFHKSRILPLPQDVTRELEHHLQVRRQHNFPVGSATPLI
                     WNRWQGGKAYSAWGLQCSLWALLDACNIRTQSGRRPRVHDFRHSFAVNVLIRWYRSGA
                     DVRTKLPFLTAYLGHVSIISTYRYLHFVEPLRALASSRFSDSYGGLVVPLSVRERSRA
                     "
     gene            complement(2142..2978)
                     /locus_tag="VFC15_06670"
     CDS             complement(2142..2978)
                     /locus_tag="VFC15_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM09878.1"
                     /translation="MASEFLVYLKLEKHPDRLETLTVNDLEAFVKKISPRFSRISLQN
                     AAGKLRSFLRFLAVMGEIPQGLDRQIDTPRVYQEEQLPRALPWETVQTLLNSIDRSSS
                     TGLRDFTMFLLMATYGLRASDVVALTLDDIHWRAGKISISQRKTGAILELPLTDAVGA
                     ALYRYLKKAPPPLPFRQIFLRRKAPIGTIKTAALSRAFRVWARRGGVDIPGKGSCHRI
                     RHSYAVFLLRKGTPIKTIGDLLGHRTLQSTSTYLRLAIEDLRDVALPIPVESKGTKAV
                     CD"
BASE COUNT          941 a         1049 c         1202 g          844 t
ORIGIN      
        1 atcgttgacc agcacgcgcg cgtgctgcgg gttgttctgt aatgttgaga actcagtaat
       61 gtggagctgc tccgcgatgt cccacctatg cggcgtttcc aggcaggcgg tctccacatt
      121 actgaggggc ttatagatct tccaaccatt taagaacgga ctggtctcga cgccaagcag
      181 ggttgcggcg ataagcgacc ggtttcacct tggcgatcgc acgccgcttc atttcgagat
      241 caatggtggc gtaccgattc gttgtgtttg gactggaatg tcccaaccac tgactgatcg
      301 cggtgatttc gacgcctgaa gtcagaagtg caacagccgt gctgtgacgc atgctgtgtg
      361 ggtgaagacg tttgcgagtg agagtaggga catcgtcgcg aacacggtcg aaatgtttcg
      421 ccaagatgta ccgcacccca aagcgagtta gcggctcctc gcgatggttg agaaagaccc
      481 gcgaatcaga gcgaaggtcc atgtggcgct cctcgcaatg atcgcgaagg acatgggcgg
      541 tttgtgacca caaagggcag aagcgttctt tcctgccttt gccgaagagc cggatttgga
      601 acggtcgtat cagttgaaga tcgcgggcgc gcagatcgac gatctcctgc acgcgcgctc
      661 ctgtgttgaa catggtcgcc agcagagcgt agtctcgtcg gccatcccga ttggctcggt
      721 tgattcgaga aaggataacg tcgatttcgg cgcgttccag gtattcgatc acgcgctgac
      781 gagcgcgttt gaagggaatg cccagaatgc gctgaacccg gtcgagatgt tcgggatgat
      841 aggttcccag gtagcgaaaa aaggcgtgga tggcggccaa gcggacgttt cgggtcgtca
      901 cgccattgtt gcggacttcc tccaaatgag ccaggaaggc aatcacctgg tccggttcaa
      961 tgtccccaag atccagttcg tgaacccggc agtttcgttg agaagccagg aaacgaagaa
     1021 agagcagcaa gctgtcacga tagctgtgaa cggtgtggac actcgttcca cgaactcgcg
     1081 gcaggtggtc tgtaaagtag tgccgcaggc gaaaagcgag cttgttcggg gatgcgatct
     1141 tcatgctctg ctcctttccc gaactgatag cgggacgaca agtccgccat acgaatcgct
     1201 gaaacggctg ctggccagag cgcggagagg ttcgacaaag tgtagataac ggtaggtcga
     1261 gattatggag acgtggccca gataggccgt caagaatggc agtttcgttc tcacatcggc
     1321 accagagcgg taccagcgaa tcagcacatt gaccgcaaaa ctgtgccgaa aatcgtgcac
     1381 ccgcggccgc cggccggatt gcgtacgaat gttgcaggcg tccaggagag cccacaaact
     1441 gcactgcaat ccccatgctg aataagcttt tcctccctgc catcgattcc agatgagcgg
     1501 cgtggcggaa ccaacaggaa agttatgctg gcgccgcact tgcaggtgat gctcaagctc
     1561 gcgagtcaca tcctgaggta gtggaaggat acgtgacttg tggaacttgg aggcgcgcac
     1621 caacagcgta cctccgcggg aatcgatgtc gccgaccaca agctggagta actctccaat
     1681 gcgtaacccc gtcgtgtaga gaagcacgac agcaagacgg accacttcgg gacggagagg
     1741 cgaatacggg ctccgctgaa ggcttgatgc ggccctcatc agccgcgcta tctcggcttc
     1801 ggaaaagatg tatggagtta ccgtttggcc aggagtcggg aaaagggttg ggtctggtac
     1861 aaagcagtcc ggcacggttc gacggcgata gaggcaaaac ttccgaacaa tcaccatgcg
     1921 gtagcgtcgc acgcgaggtg ttagcgcgga ctggctttgg caccagaaat caaagacctc
     1981 agcggttagg ctttgtgagg cacgaggaaa tccactcaag aatcgatcca gggatttaag
     2041 agccgtgatt gtgttcttgt atcgacggcc gagcgcgaag ttaagttcga ggtaccgagt
     2101 gatggtcgag cgcagtgctg atttaaagac gaatgtcggt ttcaatcgca caccgccttc
     2161 gttccctttg attccactgg tatcggcaga gcaacatctc gcaaatcctc gattgccagg
     2221 cgcaagtaag tcgaagtgct ttgcagtgtg cgatggccga gaaggtcgcc aatggtcttg
     2281 atcggtgtac ccttccgcag tagaaacaca gcatacgaat gccgaatgcg atggcacgat
     2341 cctttcccgg ggatatccac tccgccccga cgcgcccaaa cccggaatgc cctcgataga
     2401 gcagccgtct tgatcgtccc aatcggagcc ttcctgcgaa ggaaaatctg tcgaaacggc
     2461 agcggtggag gagctttctt gaggtatctg tacaacgctg ctcccaccgc gtcagtcaaa
     2521 ggaagttcga gaatagcgcc ggtctttctt tgagaaatgg aaatcttacc ggctcgccag
     2581 tgaatgtcat ccaaggttaa agccacgaca tcacttgcac gtaacccgta ggttgccatc
     2641 agcaaaaaca tcgtaaaatc gcgcagaccc gtcgaactgg agcggtcgat agagttgagc
     2701 aatgtttgaa ccgtctccca cggcagagcg cggggaagtt gctcttcttg gtaaacgcgc
     2761 ggagtatcaa tctggcgatc aagcccctgg gggatctcgc ccatcactgc caggaaccgc
     2821 aagaagctgc gaagtttccc cgcagcgttc tgcaagctaa tccgactgaa ccgcggactg
     2881 atctttttca cgaaggcttc gagatcattg acagtcaggg tctccagccg atcagggtgc
     2941 ttctccagct tgaggtatac gaggaactcc gatgccatat aggagttctg ccggatcgta
     3001 gacgccgcag ctcctcgaac agtttccaaa tagcgtgcgt acgcagctag ataagttgct
     3061 tcgacaccgg gcaccttctg ttctgtcggc ctcaagaggt tttggtctcg cagatatgtt
     3121 tccagaggcc acgttgtacc ggttagataa gggaaccgtc gcagcaggct tttgcgacag
     3181 gcagataagt cggctcggcc aatctggttc agatggcgga tgccacgccg aatcaggacc
     3241 gcttcgatat aaggcaacaa ccaaatccgc tgccttgtgt acgtatgggt gtagcggtgc
     3301 tccacgagcc aagtggtgaa ctcgttcgca atcgacccga ggatcgccaa tgatgaaaac
     3361 cgccggaagc tgcgggggta gaaagtcgta agcatcagat ctcctttgag cgctggacag
     3421 cgccaatctg atgctacgaa gaaatgtgca gttggaaatc gcagagacca gcactcacgc
     3481 tggttccatc cgttaactcc acattactga gttctcaaca ttacagaact tatgtggagc
     3541 tccacataaa ttggggaatt tgacgacata gtgatgtccg tcggcacagc gcatgagatg
     3601 gctctgcgcg ccgccgcgca tcctgcggac atgttgaacc gcttgtattg gcatgttggg
     3661 tgtccatttc ggcgacgtta tgcaaccgcg gcctgtggtt agagttcttg gggaatgagg
     3721 attcggcccg gttcagaggt tgaggcatgg cgacgggcac acgaaagcgg acggagtaag
     3781 aagactcgtg gagtgcgccc gatgaattgc cttcggctac gagtgacggt taacaggtag
     3841 ctgaagcagt gccaggacgg agagaggttc gaggagtttg cggggacggg cgcgaaacgt
     3901 tctatcggcg ccggcggctt ggtcgaaccg cggccctgcc ttcgcgaatc ggcaagggca
     3961 caagctgtcc gtggtgctag caagacacaa acccccggca ataccggaac tcagacgctt
     4021 gtgcagcgtc ttgagt
//
LOCUS       DASRKK010000308         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_902992, whole genome shotgun sequence.
ACCESSION   DASRKK010000308 DASRKK010000000
VERSION     DASRKK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902992"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFC15_06675"
     CDS             <1..248
                     /locus_tag="VFC15_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09879.1"
                     /translation="YLVQQQGIDPGRIEVRKGTGKDHVADIFWVPQGADTASAPLLQG
                     TTPVDESMVTPSENAYPKPKTAAPMHHHKKAAAAPAQ"
     gene            419..658
                     /locus_tag="VFC15_06680"
     CDS             419..658
                     /locus_tag="VFC15_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09880.1"
                     /translation="MRSSAYDLGDPNHVLLGTSHREYSQTPYGSGFFTERRGYPLGSG
                     LRRILIARNLRRTPEWDRIAQFSSANATLVLQIHG"
     gene            729..>2186
                     /locus_tag="VFC15_06685"
     CDS             729..>2186
                     /locus_tag="VFC15_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09881.1"
                     /translation="MSFCVAAVGTRRALTLIAIACALSSLGTLAPAQNPSPKDEVFGG
                     YSWLAPNGWGDLDYKINDIPKGFDASNTYYFSGAHNIGLLMDGSGHFNGATTPGNPFT
                     SNDKTGVGYALFGIQYKYHTNSLSPFVRGFVGTANISPDCCHDTECSFAAGGGGGLDL
                     RVKPRFSIRLVQADYIYSNYSHIFPSTHPTQWNSVRLAAGVVINFGNYYAPALAATCS
                     AQPTEVIEGEPITVTATGTNFNPKHTVTYAWTTNGGKLDTANAQSAKIDTTGVAAGAY
                     TANATLTDAKMRKMNSAKCSAPFTVKAKPMNPPEVTCSANPTTVRAGTPSTITANATS
                     PDPGVTISSYSYLASAGTISGTGTTATLDTTGAPAGPINVSVTATDSRGLTNAQPCVA
                     TVTVEGKPELPQVSARTPIQFIPRPKQKYIPWRVDNEAKAILDDDASALKNDPNAKLV
                     IVGYADGEPQPKIGMGKKKHAMDLAAQRAVNAKAYL"
BASE COUNT          486 a          680 c          616 g          404 t
ORIGIN      
        1 cttacctggt acagcaacag ggcatcgacc caggccgtat cgaggtacgc aagggcacgg
       61 gcaaggacca cgttgcggat atcttctggg taccgcaggg cgcggacacc gccagtgctc
      121 ccttactgca gggcacaacc ccagtcgacg agtcaatggt gacgccgagc gagaacgcct
      181 atccgaaacc gaagacggcg gctccgatgc accatcacaa gaaggcagcg gcggcgccgg
      241 cccagtaagc gagtaatttg gctggaactc taccccggtg agagcgcaat gctctcaccg
      301 gttttttgtt gtgaagggga cgaagccggg cggaatccaa tgccacgctc caccggccag
      361 caatcggggc attgcttgca ggccgcgcct cgcttgattg gaccccgaag gcggcgacat
      421 gcgcagttcg gcctacgacc ttggcgatcc caaccacgtc ctgctcggca ccagccaccg
      481 cgagtattct caaactccct atggatccgg tttcttcacc gagcgccgtg ggtatccact
      541 gggctccggc ctgcgacgca tcctaatcgc gcgcaacctg cggcgaaccc cagagtggga
      601 ccgcatcgcc cagttctcat ccgcgaatgc aactttggta ttgcagattc acggatgaga
      661 gcccacaatt ggcaatagct gggatgaaac ggaatcccgg atgactgcat caattccgga
      721 ggatcatcat gtccttttgt gtagcggctg tcggaacgcg tcgtgccctg acgctgattg
      781 ccatcgcctg cgcgctgagc tccctgggca cgctcgcgcc agcgcaaaac ccttctccaa
      841 aggatgaagt ctttggcgga tattcctggt tagcgccgaa tggatggggt gatctcgact
      901 ataagatcaa cgacattcca aaaggcttcg acgcttctaa cacttattac ttttctggcg
      961 cgcataatat cggcctcctg atggatggca gcggccattt taatggagcc accacacccg
     1021 ggaatccttt tacttcaaac gacaaaactg gcgtcggcta tgcactgttc gggatccagt
     1081 acaagtacca cacaaactcc ttgtcgccgt ttgtccgtgg tttcgtggga acggcgaata
     1141 tatctcccga ttgctgccat gacacggagt gtagtttcgc agctggcggc ggcggcgggc
     1201 tcgatctgag ggtgaagccg cggttctcga ttcgcctggt tcaggccgac tacatttatt
     1261 caaactacag tcacatattc ccaagcaccc acccgaccca atggaacagt gtccgtttgg
     1321 cggccggcgt ggttatcaac ttcggcaatt actacgcccc ggcgttggcc gcgacctgct
     1381 cggcccagcc taccgaagta atcgaaggcg agccgattac ggtcacagct acgggtacca
     1441 acttcaatcc caagcacacc gtgacctacg cctggacgac caacggtggc aagctggata
     1501 cggcgaatgc gcagtcggcc aagattgaca cgaccggcgt ggccgctggg gcctacaccg
     1561 ccaatgcgac gctcaccgat gccaagatga ggaagatgaa ctcggcgaag tgtagcgcgc
     1621 ccttcaccgt caaggcgaag ccgatgaacc cgccagaagt gacctgctcg gctaacccga
     1681 ccacggtacg cgcgggtact ccgtccacca tcacagccaa cgcgaccagt ccggatcccg
     1741 gcgtgaccat ctccagctac tcgtacctgg cttcggccgg aacgatcagc ggaaccggca
     1801 ccaccgcgac cctggacacg accggcgcac cagctgggcc gatcaatgtc agcgtcacgg
     1861 cgacggattc acgcggattg accaacgctc aaccctgcgt cgctacagtg acggttgaag
     1921 gtaagcccga actgccgcag gtgagtgcgc gtacgccgat tcagttcata ccgcgtccga
     1981 aacagaagta cattccgtgg cgcgttgaca acgaagccaa ggccatactg gacgacgacg
     2041 cctctgcatt gaagaacgat cccaatgcga agctggttat cgtcggttac gccgatggcg
     2101 aaccgcagcc gaagatcggc atgggcaaga agaaacatgc gatggatctg gctgctcagc
     2161 gtgccgtaaa cgccaaggct tacctg
//
LOCUS       DASRKK010000309         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_316649, whole genome shotgun sequence.
ACCESSION   DASRKK010000309 DASRKK010000000
VERSION     DASRKK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316649"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1001
                     /locus_tag="VFC15_06690"
     CDS             234..1001
                     /locus_tag="VFC15_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM09882.1"
                     /translation="MSDSFQSAIEFRRVEYRVPSGNVLLSDLNLGVRRGETLMLLGRS
                     GSGKTTCLKLINRLLEPSAGEVLVENICSLEWDPIRLRRRIGYAIQDVGLFPHYTVWE
                     NVALVPRLELWDQQRIAERVEEVLQLVGLPSRDFAKRYPSQLSGGQRQRVGLARALAA
                     EPPILLMDEPFGALDPITRAEIQTEFKKLQEKLKKTIVFVTHDVGEALMLGDRIALME
                     SGQLRSIHTPEEFLRAQDALVQDYVRVYREMGEMFRN"
     gene            1084..1713
                     /locus_tag="VFC15_06695"
     CDS             1084..1713
                     /locus_tag="VFC15_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM09883.1"
                     /translation="MNFLLTHKHEIAVLTGEHLWLVGVAMLFAVAIGVPLGILLTRRP
                     KLKTLILGSTNIVQTIPSLALFGLLLPLPWLGARADRLAIFALALYALLPVVRNTYVG
                     ITGISEPVREAAVAMGLTSRQLLWQVELPLASPVILAGIRVATVITIGVATIAAAIGA
                     GGLGEFIFRGIAMVDNGVILAGAIPAALMALLADSLLGGIEKLLARRQH"
     gene            1715..2641
                     /locus_tag="VFC15_06700"
     CDS             1715..2641
                     /locus_tag="VFC15_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZM09884.1"
                     /translation="MLDRRAFLSGMMLLAAGCTSRRDDIVVGSKNFTEQLILGELVVQ
                     VLARTCQTIIDGRFYLAGTYICQQAILAGRIDIYPEYTGTALAAVLKEKATGDSHEVY
                     EEVKRQYRSRFGLDVMPPLGFDNSFAMVMRGDDARRMGLTKLSQLEPVAPQLKLGVGY
                     EFLERRDGYAGLVKTYDLEFAEAPRVMDLGLLYRALENRSVDIVAGSNTDGLIAALGL
                     IVLEDDRHYFPPYDAVLIVRPEIVQRCPKARSALESLSGRITAADMRQMNYQVDGQKR
                     DAGEVAAEFLNQRLISTTVPLRRSLLKTPPKG"
     gene            2783..3223
                     /locus_tag="VFC15_06705"
     CDS             2783..3223
                     /locus_tag="VFC15_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09885.1"
                     /translation="MSKLSRHSAIRDLVSNQDVPSQEELRRRLYKRGHRVTQATLSRD
                     IHELSLVKTSEGYKLPQGEVSEAFLPSIERLIHEFVYDVKTAQNQVVVKTSAGSAQPV
                     SAAIDGEEWEEVVGTIGGDDTILVIAPDNKKAEKLASRIRGYFK"
     gene            3227..4270
                     /gene="argC"
                     /locus_tag="VFC15_06710"
     CDS             3227..4270
                     /gene="argC"
                     /locus_tag="VFC15_06710"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZM09886.1"
                     /translation="MSGRIQSAVFGATGYSGFELVRLLARHDSAKKPLLFQRNGDSGY
                     ADLSDAYPQLAGNGGFPFESFSWQRMTDAGVDVLFLATPHEFSRELVPEAISRGFRVI
                     DLSGAWRLKDAGNRAVYGFNDSDAQSAATMDAAAVYGLPELHAEAIKGAMLLANPGCY
                     PTSIILALAPWIQAGYVDVAHGVVCDSKSGVSGAGKSPTAKTHFVEVDGDFAAYSVFG
                     HRHTGEMLEQLGIDSGLLQFTPHLLPIPRGILSTIYVKLSKPVTADQLDQTLCEFYAG
                     KPWVRIFGGDKLPQIKFSRNTNFCDIGFSLSPDGTRAVLVSCLDNLLKGAAGQAVQNM
                     NLMFGFDEKAGLQ"
     gene            4267..5046
                     /gene="argB"
                     /locus_tag="VFC15_06715"
     CDS             4267..5046
                     /gene="argB"
                     /locus_tag="VFC15_06715"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZM09887.1"
                     /translation="MKLVIKIGGAALDSKELVQKFARTIPSLCKAGHQIVIVHGGGAA
                     LSRTLKQLGCETTFIDGLRVTDAQTRDVAMMVLAGQLNKLLVAAIGAAGQPAIGLCGG
                     DLGMFRATRKQSPDLGFVGDIRSVNIEWIERLWTTGIVPVVSSLALGFDGEYYNVNAD
                     QMASACAIACSAHALIYITDVSGVRDAEGVVIRWLDVKAIAQMVQKATVSGGMLPKLE
                     ACTNALKKGVTRVRILPADHAEALPGFFTQPIDWGTEVIAS"
     gene            5159..>5748
                     /locus_tag="VFC15_06720"
     CDS             5159..>5748
                     /locus_tag="VFC15_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09888.1"
                     /translation="MIATTIPAGASPSLYTQRSVSGRDLISIQDFQPDELACALELAT
                     AMKLRPGDYRGTLVGKQIVLFFEKPSLRTRLTFEAGINSLGGASFFVDQTQSRLGARE
                     SLSDVARNLERWIDGVVLRTFAHETVTEMAMHASIPVINALSELEHPCQAMADMQTLQ
                     EHFGDLHNVRLAYVGDGNNVAHSLMLAAASLGASISV"
BASE COUNT         1169 a         1601 c         1757 g         1221 t
ORIGIN      
        1 gcgctagtct ccgctccaga acttcatcaa gggcacctca tggtgccctt gatgtttttg
       61 gacattgtct ctgacttatt ggactgccaa cacactattg aaacaatcgc gttcgcaatc
      121 aaacattcca aggatttgtc cgaatgcagc tccagcgcgg gaccatgcgc agtcgacagt
      181 tctgatacgc tgatgcgaaa tgtctgactc cggcggccag tgttgattga gatatgagcg
      241 actctttcca aagcgcaatc gagtttcgca gagtcgaata tcgggtaccc tccgggaatg
      301 tgctgctttc tgatttgaac cttggcgtac gtcgtggcga aaccctcatg ctgctgggcc
      361 gcagcggctc gggtaaaacc acatgcctca agctcatcaa tcgcttgctc gaaccttcgg
      421 caggcgaggt gctggtcgaa aacatttgca gcctggagtg ggatccaatc cggctgcgcc
      481 gccgcatcgg ctacgccatt caagacgtcg gcctttttcc tcactacacg gtttgggaga
      541 atgtggcgct ggtgccaaga cttgaactgt gggaccagca gagaattgcc gaacgcgtgg
      601 aggaggtcct gcaactggtt gggttgccca gtcgcgattt cgccaagcgc tatcccagcc
      661 aactctcagg cggacagcgg caacgcgtag gactggctcg cgcattggcg gctgaaccgc
      721 cgatccttct catggacgaa ccgtttggcg ctctcgatcc catcacgcgg gccgaaatcc
      781 agacggaatt caagaagctt caggagaaat tgaagaaaac tattgtcttt gtgacccacg
      841 acgtgggaga agccctgatg ctcggcgatc gcatcgcgtt aatggaatca ggccagttgc
      901 ggagtatcca tacgccggag gaatttctcc gggcgcagga cgctctcgtc caggactacg
      961 tgagagtcta tcgggaaatg ggcgagatgt ttcgaaactg aagatcaacg gcctctgcgt
     1021 atattccgga tgcattgcca catcaggacg acccgcgaga tcggtgcctg aaggacttaa
     1081 ggtgtgaact tcctgctcac tcacaaacac gaaatcgcag tgttgactgg cgagcatctt
     1141 tggctggtcg gcgtcgcgat gttgtttgcg gtcgcgatcg gcgttccgct gggaatcctg
     1201 ctgacgcgca ggccgaaact aaaaacgctt atcctcggca gcaccaacat cgttcagacg
     1261 attcccagcc tcgcgctctt cggactcctg ctgccgttgc cctggctggg agcgcgggcc
     1321 gaccgcctcg caatttttgc tcttgcgctc tacgcgctgc ttccggtggt acgaaatacg
     1381 tatgtcggaa ttaccggaat cagcgaaccc gttcgcgagg cagcggtggc catgggactc
     1441 acctcgcgcc aactgctttg gcaggtcgag ctgcccctgg catcgccggt aattctggcc
     1501 ggtattcgcg ttgccactgt gatcacgatc ggggtggcca cgattgccgc ggcgatagga
     1561 gcgggcgggc tgggagagtt catctttcgc ggcatcgcaa tggtggataa tggcgtgatt
     1621 ctggcgggcg caattcccgc cgcgctcatg gccttgctgg cggattctct gctgggtgga
     1681 atagagaagc tcctcgcgcg caggcagcac tgacatgctc gaccgccgag cattcctttc
     1741 cggaatgatg ctgctggctg ctggctgcac atcacggagg gacgacatcg tcgtcggatc
     1801 caaaaacttc accgagcaat tgatcctcgg agagttggtg gtccaagtcc tcgcgagaac
     1861 ttgccaaacc atcattgacg gtcgtttcta cctggccgga acctatattt gccagcaggc
     1921 cattcttgcc ggccgcatcg acatctatcc cgagtacacg ggaacagcgc tcgcggccgt
     1981 gctgaaggaa aaggcgacgg gggattccca cgaggtttac gaagaggtga aacgccagta
     2041 ccgaagccgg tttggtctcg acgtgatgcc gcctctgggt tttgacaaca gctttgccat
     2101 ggtgatgcgg ggcgatgatg cgcgtcgcat gggcctcacg aaattgtcgc aactggagcc
     2161 cgtggctccc caactgaagt tgggagtggg atacgagttt ctggagcgcc gggacggtta
     2221 tgcggggctg gtcaagacgt atgacctgga attcgccgag gctcctcggg taatggattt
     2281 aggcttgctg taccgggcac tggagaaccg gtcggttgac attgtggctg gatcaaacac
     2341 cgatggactg attgccgcac tcggactgat tgtactcgag gacgaccgcc actactttcc
     2401 gccttacgat gccgtcttga tcgtccgacc cgaaatcgtt caacgctgcc cgaaggcgcg
     2461 ctccgcgcta gagagccttt ccgggcgcat taccgccgcg gacatgagac agatgaatta
     2521 ccaggtcgat ggccagaagc gggacgccgg ggaagtggct gccgagttcc tgaatcagcg
     2581 gttgatctcc accaccgtac cattgcgtcg gtcattgctc aaaacacctc cgaagggctg
     2641 aacctccaca ccgcgatcgt tggccaggca accagcccgc ggagcttcaa tcagtccggc
     2701 ccaggaaacc tcgaaaactg cttccgtcgt attgacattc caccacaatg tattattatt
     2761 catatagctg catatttatg ctatgtcgaa gctgtcccgt cattccgcaa tccgcgacct
     2821 ggtcagcaat caggacgttc ccagccagga ggaacttagg cggcggttgt acaagcgcgg
     2881 acaccgggtt acgcaggcga cgttgtcgcg cgacattcat gaattgagtc tggtgaaaac
     2941 cagcgaaggt tacaagctgc cgcagggaga ggtttccgag gcgtttctgc cgtcgattga
     3001 gcgcttgatt cacgagttcg tatacgacgt aaagacggcc cagaaccagg tggtggtgaa
     3061 aaccagtgcg ggcagcgcgc agccggtctc ggcagccatt gatggcgaag agtgggaaga
     3121 ggtcgtgggg accattggcg gagatgacac catcctggtg atcgctcccg ataacaaaaa
     3181 ggcggagaaa ttggccagcc ggatacgtgg ctatttcaaa taagcgatga gcggacggat
     3241 acaatctgcg gtgtttggag cgactggata ttccggtttc gagttggtgc gcctgctcgc
     3301 ccggcacgac agcgccaaga agccacttct gttccaacgc aacggagaca gcggttatgc
     3361 cgatttgagc gacgcgtatc cgcaacttgc gggcaatggc ggattcccct tcgagagctt
     3421 ctcctggcag cgaatgacag atgcgggcgt agatgttctg tttctggcga cgccgcatga
     3481 gttttcccgc gaactggtgc ctgaggcgat cagccggggt tttcgcgtga ttgacctcag
     3541 cggcgcgtgg cggctgaagg acgccgggaa ccgcgccgtg tatggcttca acgacagtga
     3601 cgcgcagtcc gcggcaacca tggacgctgc ggcggtttat ggtctccctg agttgcacgc
     3661 cgaagcgatc aaaggcgcga tgttgctggc caatcccgga tgctatccca cgtcgattat
     3721 tctcgcgctc gcgccctgga ttcaggccgg ctatgtggac gtagcgcacg gcgtcgtctg
     3781 cgattccaag tcaggtgtgt cgggagcagg aaagtcgccg acggccaaga cgcacttcgt
     3841 cgaggtcgac ggagatttcg ccgcgtacag cgtattcgga caccggcaca cgggagaaat
     3901 gctggagcaa cttggcatcg atagcggctt gctgcagttc acgccgcacc tgctaccaat
     3961 tccccgcgga atcctttcca ccatttacgt gaagctgtcg aagccggtga cggccgatca
     4021 gctggaccag acgctgtgcg agttctacgc cggcaagccc tgggtgcgga tattcggggg
     4081 ggacaagctg ccgcaaatca agttttcgcg caacacgaat ttctgcgaca tcggtttctc
     4141 gctttcgccg gatggcacgc gtgcggtgtt ggtgtcatgc ctcgacaacc tcttgaaggg
     4201 cgcggccggg caggcggtac agaacatgaa cctgatgttc ggcttcgacg agaaggcagg
     4261 tttgcaatga agctcgtcat caagattggc ggggctgcgc tcgacagtaa ggagttggtc
     4321 cagaagttcg ccaggacgat tcccagcctg tgcaaagcag gccatcagat cgtcatagtg
     4381 catggcggag gcgccgcctt gagccgcacc ctgaagcaac tcggttgcga aaccacattt
     4441 atcgatgggc tgcgcgtgac cgatgcgcag acgcgcgatg ttgccatgat ggtgctggcc
     4501 gggcagctca ataagctgct ggttgcagcc atcggcgcgg ctggtcagcc ggcaatcggc
     4561 ctctgcggcg gcgacctggg aatgtttcgc gccaccagga agcagtctcc ggacttggga
     4621 ttcgtgggcg acattcggtc cgtgaatatc gagtggatcg agcggctgtg gactaccggc
     4681 atcgtgccgg tggtatccag tctcgcgctc ggtttcgatg gcgagtatta caacgtgaat
     4741 gccgaccaga tggcgtcggc atgcgcgatt gcatgtagcg cacacgcgtt gatttacatc
     4801 accgacgtct cgggtgtaag ggacgccgaa ggggttgtca ttcgttggct cgacgtgaaa
     4861 gccatcgcgc agatggtgca gaaggcaacc gtgagcggcg gcatgctgcc gaagctggaa
     4921 gcttgtacca acgctttgaa aaagggtgta acgcgggttc gcatcctccc tgccgaccat
     4981 gccgaggccc tgcccggatt tttcacccaa cccatcgatt ggggcacgga ggtgattgca
     5041 tcatgaaact gcttgccttc gaccccgctc tcgagcgacg ttgttgacgt tcgatcctga
     5101 agctgccaaa caagacattt tttctttccc atctggtcga accagaagga ggtatgacgt
     5161 gatcgcaacc actattcctg caggtgcaag cccgagcctc tacacccaac gcagcgtgtc
     5221 gggccgcgac ctgatttcga tccaggactt ccagcccgac gaattggcct gcgcccttga
     5281 actggcgacc gccatgaagc tgcgtccggg ggattatcgc ggcaccctgg tcgggaaaca
     5341 gattgtgctg ttttttgaga agccgtcgct gcgcacgcgt ctcactttcg aggccggcat
     5401 caatagtctg ggcggagcgt cgttctttgt ggatcaaacg caatcgcggc tgggagcacg
     5461 cgagagcctg agcgacgtag ctcgcaatct ggagcgctgg atcgacggcg tggtgctgcg
     5521 gacgtttgcg catgagaccg tgactgagat ggccatgcac gcttcgatcc cggtgatcaa
     5581 cgcgctgagc gagttggagc atccctgcca agcgatggcc gatatgcaga cgttgcagga
     5641 acacttcggc gatctgcata atgttcgcct ggcgtatgtg ggtgatggca acaacgtcgc
     5701 tcattcgttg atgctcgcgg ctgcgagcct tggcgcatcg atctcggt
//
LOCUS       DASRKK010000310         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_708023, whole genome shotgun sequence.
ACCESSION   DASRKK010000310 DASRKK010000000
VERSION     DASRKK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708023"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFC15_06725"
     CDS             <1..259
                     /locus_tag="VFC15_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09889.1"
                     /translation="GDEDGFISILVTLTDSAGNNTQFSSSLQPVPAGGDFTDSHLLFL
                     DASYDTPGQINVTMRIEFSGALSDVKFAECSFNVTDGQPSP"
     gene            complement(348..959)
                     /locus_tag="VFC15_06730"
     CDS             complement(348..959)
                     /locus_tag="VFC15_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM09890.1"
                     /translation="MAVTFSGEFTSPRSPDEVYDFLCDPNKFGPLLPEFRSMTLQDAT
                     HFTVTVGVGVGNIRGTAEIKMELSEAVRPTRAQYKGRGYAVGSQITIGAGFDLSPVDG
                     STRIAWRAEAGVFGKLASMAGGMLEPLGKKNIQKLIDGLQRALTVAAKPVAEEATTEE
                     ARATPAKQPAQAIPQQVPGPEPQTSPAEQAPQPAADSTVSRLG"
     gene            complement(962..1180)
                     /locus_tag="VFC15_06735"
     CDS             complement(962..1180)
                     /locus_tag="VFC15_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZM09891.1"
                     /translation="MYPRVFEHYRAKSYPEAVTLLRQLGKEAKLVAGGQSLTPLMKLC
                     LSAPRHLIDPYQSIFKAIQAAATAGPRS"
     gene            complement(1263..>2202)
                     /locus_tag="VFC15_06740"
     CDS             complement(1263..>2202)
                     /locus_tag="VFC15_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZM09892.1"
                     /translation="MAREGGMLRSLFIGLSESKRLRHFAEQSPLGLRFSQRFVAGTHL
                     HDVIRATQVVNEWGAGVSIDNLGENVTNADESKHSAELYHRLLDEIHQRKLNANVSLK
                     LTHMGFDVSEDLAFGLVTELVDHAVRINNFIRVDMEGSPYTQRTLEFVRGLHREPGHA
                     GRVGTVIQSYLYRSEKDVDELLSERIRIRLCKGAYKEPPEIAFPKKADADANYLKLAR
                     TLLKSGVFHGIATHDEKIIAEIKQFARAENIPNSAYEFQMLHGVRRDLQQQLVREGYG
                     LRVYIPFGSEWYPYFMRRLAERPANVIFIARNMFRQ"
BASE COUNT          388 a          674 c          664 g          476 t
ORIGIN      
        1 gggtgacgag gatgggttca tctctattct tgtcacgcta accgattctg ctgggaacaa
       61 cacgcagttt tcttcaagcc ttcaaccggt ccctgccggg ggcgatttca ccgatagtca
      121 cttgcttttc ctcgatgcaa gttatgacac gcctgggcaa atcaatgtaa ccatgcggat
      181 cgagttctcg ggagccctct cggatgtgaa gtttgcggag tgcagtttca atgttaccga
      241 cggccagccc tccccctagc acaaggggcc ggagacaggg gcgcgctcag tttcgtttga
      301 aatgacccct tcaaaacttc ccgtccacag cgcctgacac ccgtgaatca tccaaggcgt
      361 gaaactgtgc tatccgctgc cggctgtggc gcttgctcag ccggcgaggt ttgtggttca
      421 gggcccggta cttgctgcgg aatggcctgc gccggctgtt ttgcgggcgt ggcccgcgct
      481 tcctcggtcg ttgcctcctc cgctacaggc ttcgccgcca ctgtcagcgc ccgttgcagt
      541 ccatcaatca gcttctggat gttcttcttt cccagcggct caagcatgcc gcccgccatg
      601 gaggccagct tgccaaatac accggcctca gctcgccacg cgatcctggt gctgccgtca
      661 acgggagaga gatcgaaccc ggcgccgatg gtgatctggc tgcccacggc atagccccgg
      721 cctttatatt gcgcccgcgt tggccgcacg gcctcgctga gctccatctt gatttctgct
      781 gtccctcgaa tgttgcccac gccgacgccg actgtcaccg tgaagtgggt cgcatcctgg
      841 agcgtcatgc tgcggaattc gggaagcagc gggccgaact tattgggatc gcagagaaag
      901 tcgtagactt cgtccggact gcgcggcgat gtgaactcgc ctgaaaacgt gacggccacg
      961 gttagctcct cggccctgcc gtagcggccg cttgtatcgc tttgaagatg ctctgatacg
     1021 ggtcgatgag atgccgcggc gcggagagac aaagtttcat cagcggggtg aggctctggc
     1081 ctcccgcgac aagcttggct tctttgccca actggcggag caacgttact gcttcgggat
     1141 acgattttgc ccggtaatgt tcaaagactc ttgggtacat tccgcccccg cgacccaccc
     1201 caacaagccc gccacggcgg ggcgcttgtt ggggacccgg cgacgccggt cttccgcagc
     1261 cgctattgcc ggaacatgtt ccgggcgatg aagatcacgt tggcggggcg ctccgccagg
     1321 cgtcgcatga agtacggata ccactccgac ccgaacggaa tatagacgcg caagccgtag
     1381 ccttcgcgca ccagttgctg ctgcagatcg cgacgcacgc catgcagcat ttgaaactca
     1441 tacgcggagt tcgggatgtt ctccgcgcgc gcgaactgct ttatctcggc gatgatcttc
     1501 tcgtcgtgtg tggcaatgcc atggaagacg ccgctcttca acagcgtgcg cgccagcttc
     1561 aggtagttcg catccgcgtc ggccttcttg gggaacgcga tttccggcgg ctctttgtac
     1621 gcgcccttgc acaagcggat gcgaatgcgc tctgaaagca gctcatccac atccttctcg
     1681 ctgcggtaga ggtaggactg gatgaccgtg cccacccgtc cagcgtgccc cggttcgcga
     1741 tgcagtccac gcacaaactc gagcgtgcgc tgggtgtagg gcgagccctc catgtccacg
     1801 cgaatgaaat tgttgatgcg tacggcgtgg tccaccagtt cggtcaccag gccgaacgcg
     1861 aggtcttcgc ttacgtcgaa gcccatgtgc gtgagtttga ggctgacgtt ggcattgagc
     1921 ttgcgctgat ggatctcgtc gagcaggcgg tgatagagct cggcgctgtg tttcgactcg
     1981 tcggcgttgg tgacgttctc gccgaggttg tcaatgctga cgccggcgcc ccactcgttg
     2041 accacttggg tggcgcgaat tacgtcatgg aggtgggtgc ctgcgacaaa gcgctgggaa
     2101 aaacgcagcc ccagcggcga ctgctcggcg aaatggcgta gtcgtttgct ttcggaaagc
     2161 ccgatgaaga gcgaccttaa catgccgccc tcccgggcca tg
//
LOCUS       DASRKK010000311         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1392174, whole genome shotgun sequence.
ACCESSION   DASRKK010000311 DASRKK010000000
VERSION     DASRKK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392174"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /gene="ssrA"
                     /locus_tag="VFC15_06745"
     tmRNA           <1..83
                     /gene="ssrA"
                     /locus_tag="VFC15_06745"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(245..1747)
                     /locus_tag="VFC15_06750"
     CDS             complement(245..1747)
                     /locus_tag="VFC15_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM09893.1"
                     /translation="MRLFLRWLFRLALGAIALAAITVVVVLVVHARQLDRLKRLAGIP
                     PSVYIVEQSPSIVLDHVRVIDGSGGPPREDQSIVIGSGKITYAGPSAGRPNLPTSKVL
                     DLTGRTVFPGLVGMHEHLFMTAAIPSRKSLLAQQSTAFPLMYLASGVTTMRTAGSIAP
                     EQDLLIKQRIDQGEAVGPEMFLTAPYLEGRPAMFPEMHVLEGPEEARRAVDTWADRGM
                     TSFKAYMTITPDELQAAVTEAHARGLKITGHLCTIGFRQATDLGIDNLEHGLLVDTEF
                     YSKKQPDTCPGRRATLDWMAEYNNQLDVESPPVQDMIRYLVSHHVVVTSTLAVMEEAS
                     GYPRHPEDTDRARRAMTWQAWLFSRMKIALAARFAHLDRLLGKEMAFEHDFVKAGGAL
                     LAGSDPTGPGSVLAGFADQREVELLVQAGFTPVEAIHIATQNGAEFLGIGSRVGTISP
                     GKQADLVVVEGDPSRNISDIRNVEMVFRNGIGYNPAKLLAGIDGVVGLKD"
     gene            2310..2651
                     /locus_tag="VFC15_06755"
     CDS             2310..2651
                     /locus_tag="VFC15_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HZM09894.1"
                     /translation="MNVSITDRLAGYVRKKVKSGRYNNASEVVREALRRMEDDDARTV
                     RLAAPSAEDILSDLTGQQLESIRQRVRSGIESIEDGEFKEYKGRQGLKELADEVKARG
                     RRLLAREDPGR"
BASE COUNT          606 a          776 c          738 g          588 t
ORIGIN      
        1 ccggcgcatg aacgtgacta agtatgtagg ctctttgctg acaacatttt cggacgcggg
       61 ttcaactccc gccgcctcca ccaagttgtt tattttaaat agtttacacg tgagatagcc
      121 agctccagcc cgttggattg attccagtca ctcgggagca acgcgctagt ttgcgcgcca
      181 ggaattgacg tgcataacgg caccactttg atgtgtgtag cctgttcagc ggtgggtgag
      241 tccgtcaatc tttaaggcca acaactccat cgatgcctgc gagtagtttc gcgggattgt
      301 aaccaatgcc attgcgaaag accatctcca cgttgcgaat atctgaaata ttccgcgaag
      361 ggtcgccttc gaccactact aaatcggctt gcttaccagg gctgatcgtt cccacccgac
      421 taccgattcc aagaaactct gcgccgtttt gggttgcaat gtggattgcc tcgaccgggg
      481 tgaatccagc ctgcaccaga agctcaacct cgcgttgatc agcaaatccg gctaggacac
      541 tgcctggccc tgtcggatcg cttcccgcga gcaacgcgcc tccggccttc acaaagtcat
      601 gctcgaacgc catctctttc cccagcaaac gatcaagatg agcaaagcga gccgccaagg
      661 cgattttcat acgcgaaaaa agccatgcct gccaggtcat tgcgcgtctt gctctatccg
      721 tgtcttccgg gtgcctcggg tatcccgatg cttcttccat tacagcgaga gttgaggtca
      781 ctactacgtg atgactcacc agatagcgaa tcatgtcctg taccggtgga gattccacgt
      841 cgagttggtt gttgtattcg gccatccaat ccaaagtggc tctccttccc ggacaagtat
      901 cgggctgctt cttcgaatag aactcggtgt caacaagcaa gccatgttcg agattgtcaa
      961 ttccaaggtc ggtcgcttgc cggaagccta tcgtgcagag gtgccccgta atcttgagac
     1021 cacgcgcatg ggcttccgtg acggcagcct gtagctcgtc cggggtgatc gtcatgtacg
     1081 ccttgaaaga tgtcatgccg cggtcggccc atgtgtccac cgcccgacgc gcttcctcgg
     1141 ggccctccag gacatgcatt tcagggaaca tcgctggccg gccttcaaga tatggcgccg
     1201 tcagaaacat ctccggaccc acagcctcgc cttggtcgat gcgctgtttg atgagcaggt
     1261 cctgttccgg cgcaatgctg cctgcagtgc gcatcgtcgt cacgcccgat gcgaggtaca
     1321 tcagcggaaa agctgtggac tgctgcgcca gaagggactt tctactggga atcgccgcag
     1381 tcataaacag atgttcgtgc atgccgacca gacccgggaa caccgtccgt ccagtgaggt
     1441 caagcacttt gctcgtcggc aaattcgggc gtcccgcact ggggcccgcg taggtaatct
     1501 tgccggaccc gatcacgatg ctctggtctt cccgaggtgg cccgccgcta ccgtcgatca
     1561 ctcgaacatg atccagaaca atcgatggag actgttccac gatgtacaca gaaggcggga
     1621 tgccagccag ccgcttgagt cgatcgagtt gccgggcgtg aaccaccaga acgactacca
     1681 ccgtgatcgc agccaacgcg attgcaccca atgcgagccg gaaaagccaa cggaggaaga
     1741 ggcgcatttt tcaccccacg atcaagatgc aaactgtctc acgagaggat aaaccgggca
     1801 acttggccag tctatgggaa tctcgtacca aacactcgga gcgccgagaa cctgcggaaa
     1861 cgattgatcc gttccgggat gacatgccga gtgctggaag cgacgagggt cgagcgtttc
     1921 gtgtggtcaa tacacggtca atgatttgcc cactttcgag tgttctgcgt atcgtgcgcc
     1981 cctaaagatt caataagttc cctgttttca acgcaacgca aaagttcaac tcccgccgcc
     2041 tccaccaatt tcaagtgctt cataatcagc tgatcaacgt tgggcagttg tctccctgcc
     2101 gcattccgct aactgccccg ccctagccaa aatcgggcta gaacggggca cccaaatttg
     2161 attcccggat aaattcgata cctgccggag cttcggtggc ccacccgcct ttccatccct
     2221 tggcgaactg tatatcaaag attgacatcg aagcatctta cggctaggtt tgagaacacg
     2281 aagatgcagg aggggggaca gtggatcaga tgaacgtatc gattacggat cgtctcgcag
     2341 gctacgtgcg taagaaagtg aagagtggcc gctacaacaa cgccagcgag gtggtgcgag
     2401 aggctttgcg gcgcatggag gatgatgacg cgcgaaccgt tcgcctggca gcccctagcg
     2461 ccgaggatat actttccgat ctcaccgggc agcagctcga gagtatccgg cagcgtgtcc
     2521 ggtccggcat cgagtcgatc gaggatggcg aattcaagga atataagggc cgccaaggcc
     2581 tgaaagagtt ggcggacgag gtgaaagcac ggggccgtag actgctcgca cgggaagacc
     2641 ctggccgcta atgcgctatc gtgtctccaa agatgctggt cgcgatctcg atgagatttt
     2701 tctttatt
//
LOCUS       DASRKK010000312         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_316816, whole genome shotgun sequence.
ACCESSION   DASRKK010000312 DASRKK010000000
VERSION     DASRKK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316816"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1533
                     /locus_tag="VFC15_06760"
     CDS             247..1533
                     /locus_tag="VFC15_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZM09895.1"
                     /translation="MALATANAQSIEIGSTGLNRWWRVVGGLSMNLALGTLYAWSLFV
                     PELSKDPEFKMHKLPDFTWTFTIAVVVFALTFIVAGRLQDKFGPFWISLTGGVLVSLG
                     FFLSGYAHSLMALYISFGVIGGLGNGFGYATPIPVMAKWFPDKRGLAVGLAVGGYGAG
                     SAIFGKLALNYLVPAYGWRSTFEILGGIFLVMTLFGAFLLKNPPAGYKPAGWTPAPAT
                     AKASATTYEYTPSEMLRTPTFYFMWVAYALGCSAGLMVISVLIPFFKESLKNTGMSPA
                     LIASYAGTAFIVGAVGNASGRILSGWMSDHLGRINVLRLMIGISMVAMPLLYLVGGNL
                     GLLFAMIFIVYWCYGTQLSVNGAATADFFGTKNAGINYGLLFTAWGVAGVVGPIMGSK
                     LRVAYSSYKAPFYAAAILAAVALLAELMARRPAKQS"
     gene            1631..>2081
                     /locus_tag="VFC15_06765"
     CDS             1631..>2081
                     /locus_tag="VFC15_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZM09896.1"
                     /translation="MATATTSPNLDVTKEAMDQPTAHAATHGLVSPVVQKWIDEGKND
                     PDALWGRAANELHWFRKWDRVLEWTPPTFKWFVGAQTNLAYNALDYHVKRGWGGHAAL
                     VYLNERGERRTYTYAHLLHEVERVAAALRGMGIKKGDRLTVYMPTSPE"
BASE COUNT          395 a          574 c          624 g          488 t
ORIGIN      
        1 tctcctctac cccaagtcgc ctcccgccga cggttttgtg actcggcctg atccagccca
       61 gaccaggcag gggtggtacg cagcacacag gttcttagga ggtcgcgttc gtgatggcca
      121 atgaaccccg ggacagccgg cccatacctc gcaccagttc cctcctaagt cgttcggcgc
      181 agcaattatt gctctcagga aaccacgata gtaactttct tgctccgtag gaagggagaa
      241 attgaaatgg ctttagcaac agcaaatgct cagtcgattg agattggcag caccggcttg
      301 aaccgttggt ggcgtgtggt cggcgggctc tcgatgaatc ttgccttggg cacactgtat
      361 gcctggagct tgttcgttcc ggagcttagc aaagatcccg aattcaaaat gcacaaactt
      421 ccggacttca catggacctt caccatcgcc gttgtggtgt tcgctctgac gttcatcgta
      481 gctgggcgtc tccaggacaa gttcggaccc ttctggatat ctctcaccgg cggcgttctg
      541 gtaagcctgg ggtttttcct gtccggttat gcccatagtc tgatggctct gtacatctct
      601 ttcggcgtga ttggcgggct gggcaatggt ttcgggtacg cgacgccgat tccggtgatg
      661 gccaagtggt ttcctgacaa acgcggattg gccgtgggac tggctgtcgg cggctacggt
      721 gccggttcgg ccatcttcgg caaattggcg ctcaactacc tggttcccgc ttacgggtgg
      781 cggtccacct ttgagatcct gggagggatc tttttggtga tgactttatt cggggcgttc
      841 ctgctgaaga atccgccggc tggttacaaa cccgcgggtt ggacgccagc gccggcaacc
      901 gcgaaagcgt ctgccactac ctacgagtac acgccctcgg agatgctgcg tacccctacg
      961 ttctacttca tgtgggtagc gtacgcactg ggctgctccg cgggtctaat ggtgatcagc
     1021 gttctcatcc ccttcttcaa ggagagcctg aagaacactg gtatgagtcc cgctctgatc
     1081 gccagttacg ctggaacggc atttatcgtt ggcgctgtgg gcaacgcctc cggacgcatt
     1141 ctttcgggat ggatgtccga ccacctgggg cgcatcaacg ttctacggct gatgatcgga
     1201 atttctatgg tcgcgatgcc gctcctttac ctggtgggcg gtaatctcgg attgttgttc
     1261 gcgatgatat tcatcgttta ctggtgctac ggcactcagt tgtcggtgaa cggcgccgcc
     1321 actgccgact tctttggcac caagaacgct ggcattaact acggcctgct gttcacggcg
     1381 tggggtgtag caggcgttgt cggtccaatc atgggttcga agctgcgtgt ggcgtatagc
     1441 agctataagg cgccattcta cgcggcggcg attctggcgg cagtggcgct attggccgaa
     1501 ttaatggcgc gtcgtcctgc taagcaatcg tgagatcgtg gccggactgc taaaacggtc
     1561 cggccaaatt tttggttgat tttgacgggt actcataccg ccgctattga ttgaaaagga
     1621 gtgtcttcct atggctactg caacaacatc accgaacctg gacgtaacca aggaagcgat
     1681 ggaccagccc accgctcatg ccgccacgca tggcctggtc agtccggtgg tgcaaaagtg
     1741 gatcgatgaa ggcaagaatg atccggacgc cctttgggga cgcgcagcaa acgagctgca
     1801 ttggttccgt aaatgggacc gcgtgctgga atggacgccg ccaaccttca agtggtttgt
     1861 cggagcgcag acgaacctgg cgtacaacgc gctggattat cacgtcaagc gcggctgggg
     1921 aggacatgcc gccctggtct acctcaacga acgcggcgag cgtcgcacct atacgtacgc
     1981 acatttgctg catgaggtgg agcgggtggc ggcggctctg cgcggcatgg gcatcaagaa
     2041 gggcgatcgc ctcactgtgt acatgcccac cagtccggag g
//
LOCUS       DASRKK010000313         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_512765, whole genome shotgun sequence.
ACCESSION   DASRKK010000313 DASRKK010000000
VERSION     DASRKK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512765"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFC15_06770"
     CDS             complement(<1..1112)
                     /locus_tag="VFC15_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09897.1"
                     /translation="MITSSQAQTFTVLHNFTGGADGYDPSAGLTIDRAGHLYGTTLFG
                     GHTGGTCAGFGCGTVFRLTQQGSGWLFTPLYSFQGGDDGSVPSAGVVFGPNGRLYGAT
                     QYDGNDQCSAPYGCGTIFDLAPPAHACGRALCGWTETVDYRFNSGSSAESPTGNLIFD
                     GAGNIYGTAAGGGLPNCYAGTTCGVVYELSPSQGGWSETDLYRFTAGNDGYLPASGVV
                     RDQTGTLYGTALYGGNNGCGTVFQLTPSGSGWSETTLRDLQSQTDGCGPNELISDQQG
                     NLYGTDRRNGPNGGGAVFELTKLSGGGFSFRVLYALSGMPGGPMAGLTMDSAGNLYGT
                     TYQNGGNHYGSVFKLTKAGSGWTYSDLYDFTGGSDGA"
     gene            complement(1696..2232)
                     /locus_tag="VFC15_06775"
                     /pseudo
     CDS             complement(1696..2232)
                     /locus_tag="VFC15_06775"
                     /inference="COORDINATES: protein motif:HMM:NF033587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          514 a          670 c          685 g          534 t
ORIGIN      
        1 gccccatcgc tgccgcccgt gaagtcgtag aggtcgctgt aagtccaacc gctacccgcc
       61 ttagttagct tgaaaactga gccgtagtgg ttccctccgt tttgatacgt cgtgccgtaa
      121 agattgccgg ctgagtccat ggtgaggccc gccatgggtc cgcccggcat tccggaaagc
      181 gcatacagca ctctgaagga aaagccgcca ccagaaagtt tcgtcagttc aaaaaccgcg
      241 ccgccgccat ttggtccgtt cctccggtcc gtaccgtaca gattgccctg ctgatcggag
      301 attaattcat tcgggccgca tccgtcggtt tggctttgaa gatcgcgtag agtcgtctcc
      361 gaccagcccg aacccgaggg ggtcaactgg aaaaccgtgc cgcaaccgtt gttgccgcca
      421 tagagagcgg taccgtagag agtgcctgtc tggtcgcgga cgacaccgct cgccgggagg
      481 tagccgtcgt ttcccgctgt aaacctgtac aggtcggtct cactccaacc gccctgggac
      541 ggtgacagtt cgtagacgac accgcaggta gttcccgcat aacagtttgg caaaccgccc
      601 cccgcagcgg tgccgtagat attgcccgct ccgtcgaata tgagattacc ggtgggactt
      661 tcagcgctgc tgccgctatt gaaacgatag tccacagttt ccgtccagcc acacagcgcc
      721 ctgccgcaag catgtgctgg aggagctaga tcgaaaatcg tcccgcagcc gtaaggtgca
      781 gagcattgat cgttcccgtc atactgggtc gcaccataaa gcctcccatt cggaccgaaa
      841 acgacgccag ccgaaggaac agacccgtcg tcccctcctt gaaagctata cagaggagtg
      901 aagagccagc cggaaccctg ttgggtcaac ctgaagacgg tgccgcaccc aaatccggcg
      961 cacgtaccac cggtgtggcc tccgaaaaga gttgtgccgt acaggtgccc cgccctgtca
     1021 atggtcaatc cagcagaagg atcatatccg tccgctccac cggtgaagtt gtgaagcacg
     1081 gtaaaggtct gcgcctgact cgatgtaatc atgcagaccg cgaggacaat cgggcacact
     1141 aaggacagga ctaactgagt acgcgctagc cgtggcatgg tagattcctt gttcgttgtg
     1201 atatcggctg ggagcgattg tttcgtggcc gtttgcagat tctggtcttg ggggtgggtc
     1261 gctgtcaatc cgtttgtcga gtaggcaaca gtctaggtcg caacgaagtt gataactgtt
     1321 gataacaggt cagcatgacg gtgcccgtca caaccaagca tgaaaaggcc ggccagatta
     1381 gtcgcccggt ttcaataagt tgccgctctt ttcaagggag gccatatcgt aagcgatcct
     1441 gaaaccagac ggctcggcgg gtagtgtcct aacagtgcgt tgattcgata aagggagtgg
     1501 cctgggcagc aactctggcc gagactgctg gaggccactc gcgccacgtc gtctgccggt
     1561 cagattactc cacggcagag cgcaagcgca agcggtagcg agtcatggta gtggtgcttt
     1621 agcgtggaga tgaattggta gtgttgcaag tttgaaatgc agacaagttt gtcgccgagc
     1681 gtctgtcgtg cgttcttagg ctgccaagtc gaaaaggctg tcgatgaact gaatctgtcg
     1741 aagaagatca tctcctgcca cccatcgagc ttgccccttc cggatcatgt ggattgcttc
     1801 gtagccttga atcgttcgcc gagccgcctg gaattctcgg aagccctgtt tggcgttcac
     1861 tcgacgtttg atggctcgat gatcctgttc gagaatgttg ttcaagtact gcacaggtcg
     1921 gtgtcgacag cggttgcgca gcgtcccttc cttcttagtg tcggggatcg cggagctgta
     1981 tatcggcgcc agatcagtgt tgatcactcg aggttgggga taggatcgat cgctgagcgc
     2041 cttgcgaaac agtcgcttgg cagcatcggc gtcacgcaac ggtgacagca agaaatcgat
     2101 ggtcgcaccc ttggaatcga tggcccgata caagtagcac cagcgaccct tcacgcgcac
     2161 gtaggtttcg tcgactcgcc aagacttgtt tgtcggcttg agatgctttc gcagtcgctg
     2221 ctccaactca gggtcgtaat actgctgtcg ttcgccaaaa tcagatcgcc agaacgatgt
     2281 tgacttttca acaacctaga gggaacaatc agccgacatg tccgcattcc tactcggttg
     2341 gtttcggttg atttggctgt tcaccagagg ccatcaagcc ctcgttctgg agaacctcgc
     2401 act
//
LOCUS       DASRKK010000314         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_708124, whole genome shotgun sequence.
ACCESSION   DASRKK010000314 DASRKK010000000
VERSION     DASRKK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708124"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFC15_06780"
     CDS             <1..1356
                     /locus_tag="VFC15_06780"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZM09898.1"
                     /translation="ANFFISGSIVFTAAILAALLATIRSKFAMTWLGLLVAIILAASV
                     MTNHAASRMDGRAPLIVLSFLHELATGFWIGGLPFLLLGLFVGKTSSTQWRLTRRFSQ
                     IALVSVAVIVFSGVAMSLSYIGSVRSLFGTAYGVMVSAKAIMLGAMLLLGGINFLLLR
                     KYSGDAVGPRLRRLVEAEVGIGITIILTAASLTSQPPAVDLVNDTVDFAHIASRLKPH
                     WPRLASPELAQLSSPFPGRKIDIPAVAPLPAAYTIDGAPLSRSEVADIGWSEYNHHWM
                     GVLVLAMGLLALLARTGHAKWAEYWPLVLIGIAIFIFARGDPECWPLGPQGFWISWTK
                     TEVFQHRMAALLCIAFAIFELRVRRRPSSGGALALVFPLLCAAGGALLLTHSHSLTNV
                     QEEFLAEMSHVPLGILAVLSGWARWLEIRLPPEDRAIPAWIWPVCFALIGVGLLNYRE
                     I"
     gene            1765..>2225
                     /locus_tag="VFC15_06785"
     CDS             1765..>2225
                     /locus_tag="VFC15_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZM09899.1"
                     /translation="MKKRVVITGMGVVSPNGIGNEAFCRAVLAGSSGVKRIRSFDPSD
                     LPVQIAGEVQDFDELAWVDAHERKHVSRVVPLALAASTEALADAGIDSAKLSLEQQRE
                     IGVVLGSGGGAQEFSEEQVRLWLTGKVKQVSLFCIPSGTMGTLSSEVSMRFG"
BASE COUNT          399 a          653 c          679 g          494 t
ORIGIN      
        1 gcgaacttct tcatttccgg cagcatcgtt tttaccgcgg caatcctggc agcgttgctg
       61 gccaccatac gcagcaagtt cgccatgact tggttagggc tgctggttgc catcatcctc
      121 gcggcatcgg tcatgaccaa ccacgctgcg tcacggatgg acggccgcgc gccgttgatt
      181 gtcctcagct tccttcacga attggccacc ggattttgga ttggcggtct gccgtttctg
      241 ctcttgggtt tgttcgtcgg caagacgagt tccacgcaat ggcgcttaac ccggcggttc
      301 tctcagattg cgctggtaag cgttgcggtc attgtcttca gcggcgtagc catgagtctc
      361 tcctacattg gctccgtgcg ctctctcttc ggaacagcct atggagtgat ggtctcggcc
      421 aaggcgatca tgctgggggc catgctgctg ctcggtggga ttaacttttt gctgttgcgc
      481 aaatacagcg gcgacgcggt cggcccccgg ttgcgccgcc tggtcgaggc agaggttgga
      541 atcggtatca ccatcatcct caccgccgcc tcgcttacgt cgcaaccacc ggcggtcgac
      601 ttggtgaatg ataccgtcga ttttgcccac attgcttccc gcctcaagcc ccactggcca
      661 aggctggcga gtccggaact ggcgcagctt tcgtcccctt ttcccgggag gaagatcgac
      721 attcccgcgg tggccccatt gcctgcggcg tataccatcg atggagcgcc gctctcccgc
      781 agcgaagtgg cagacatcgg gtggtcggaa tacaaccatc actggatggg cgtgctggtg
      841 ctggccatgg gtctgcttgc tctgctggcg agaacgggcc acgcgaagtg ggccgaatat
      901 tggccgctgg tgctgatcgg gatcgccatc ttcattttcg cgcgcggcga tccggaatgt
      961 tggcccctgg gcccgcaagg cttttggata agctggacga aaacagaagt gttccaacat
     1021 cgcatggctg cgctgctctg catcgccttt gccatctttg aattgcgcgt ccgccggcgt
     1081 ccctccagcg ggggcgcgct ggcgctggtt ttcccgctcc tctgcgccgc cggcggagcc
     1141 ttgctgctga cccattccca ctctctcacc aatgttcagg aggaattcct ggcagagatg
     1201 agccatgttc ccttggggat tttggcggtg ctctccggct gggcgcgctg gctggaaatc
     1261 cgcctccccc ccgaagatcg cgctattccc gcctggatct ggccggtctg cttcgcgctg
     1321 atcggcgtcg gcctgctgaa ctaccgggag atataggctg cccggccacg atgcaatcca
     1381 ccgattgaac ttcattgaat aaacttagcg aagttgcaga agctgcacct cgtgcggcag
     1441 aacggcgccg agatgtgaat tccttgtgac aactgattgg cggaaaccaa ggtcgaggta
     1501 atagagtagc aactagccac tagcaatgag ctgttagaga aactccgaac aagtcgatgc
     1561 ttgggtagag ccggccttca ggccggcgtg caagctgtta taaatacttg ggggcttcag
     1621 ccccgggaac gcagaaaact gactttttgg agcttcttag catctagcat gtgacgaatg
     1681 cgaacagagt ccggatagcg cctctggttg ccagcttaag ctttcgtgca actgtgcggc
     1741 aatgcaccat ctcagagcca ctttatgaaa aagcgagtcg tcatcacggg aatgggcgtg
     1801 gtcagcccaa acggtatcgg caacgaggcg ttctgccgcg ctgttctggc cggaagcagc
     1861 ggcgtgaagc gcatccgcag cttcgatccc agcgacctgc cggtgcagat cgccggcgag
     1921 gtgcaggatt tcgacgagtt ggcctgggtt gacgcgcacg agcgcaaaca cgtctcgcgc
     1981 gttgtgccgc tggcattggc cgcgtccacc gaagccttgg ccgatgccgg catcgattcc
     2041 gcgaaactct cgctggagca gcaacgcgaa attggtgtgg tgctgggctc aggcggtggc
     2101 gcgcaggagt tttccgaaga gcaggttcgg ctgtggctga cgggaaaggt gaaacaggtc
     2161 agcctgttct gcattcccag cgggaccatg ggcacactct ccagcgaagt cagcatgcgc
     2221 ttcgg
//
LOCUS       DASRKK010000315         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_708167, whole genome shotgun sequence.
ACCESSION   DASRKK010000315 DASRKK010000000
VERSION     DASRKK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708167"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFC15_06790"
     CDS             complement(<1..1014)
                     /locus_tag="VFC15_06790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZM09900.1"
                     /translation="MNFNVLPSLVALAILVVVFRAILRQGAAERLQLWLTAWILVLIH
                     FVAKFVDVGHGPWDHVASAVNLGALELASIAFLISMSPRASTRRRQLLLGAVLGFPAL
                     AYTTAVVWELTAHSFYYGVVVVALTATSLLAWNWYRETPSYLFGLLLASVVFSSVLIW
                     MVARNRADLGINVILAGLNFVVAVLYWRHFQRATAGVLTTVFGFVAWGAVFPISMFLQ
                     LYSPSTIVESETWNIPKYFVAVGMILTLLEDQIHKNEYLAYHDALTGLPNRRLLEDRL
                     EQALAHAARAGRKVAVLLLDLDRFKEVNDTYGHRIGDLALQQVVLRLSSRMRASDTLA
                     RSGG"
     gene            complement(1444..>2254)
                     /locus_tag="VFC15_06795"
     CDS             complement(1444..>2254)
                     /locus_tag="VFC15_06795"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZM09901.1"
                     /translation="SAQAGVTAAQKQAEAAQAQILEAQANAHKLNTDVERYRQLLGKR
                     EISQQQFDQATAAAAAANATVSARQAALLAAQAQVKQAQSRIEQATAEVRNARATPNM
                     VAATRAKAKSADALAQRSKTALDQAQLNLAYATIVAPVDGVVGKRSVQVGANISSGQD
                     LMAIVPLSDIWVTANFKETQLAHMRPGQPAKIKVDTYGGRKWDGHVTSIGGATGAKYS
                     LLPPENATGNYVKVVQRIPVRIDFDGNDKPDFNKDGLLRPGMSVGPDVKVH"
BASE COUNT          498 a          624 c          667 g          465 t
ORIGIN      
        1 gcctcctgaa cgagcgagcg tgtcggaggc gcgcatccgg ctggacagcc gcaataccac
       61 ctgctgtaag gccaaatctc cgattcggtg tccgtaggtg tcgttcactt ccttgaagcg
      121 gtcgaggtca agcaaaagca cagccacctt gcggccagct cgcgcagcgt gagccagggc
      181 ctgttccaat cgatcttcga gcagtcgccg gttaggcagc ccggtcaatg cgtcgtgata
      241 tgccagatat tcattcttgt gaatctggtc ttcgagaaga gtgagaatca tgccaacggc
      301 cacgaagtac ttgggaatgt tccaggtctc ggattcaacg atcgtcgaag gagagtagag
      361 ctgcagaaac atgctgattg gaaaaactgc accccaggca acgaagccga aaaccgtagt
      421 cagaacgcct gccgtagcgc gctggaaatg gcgccagtac agaacggcaa caacgaagtt
      481 aaggcccgcc aggataacgt tgatacccag gtcagcacga ttgcgcgcaa ccatccagat
      541 caatacggag gagaacacca cggacgcaag cagcaggccg aacagatagg aaggtgtctc
      601 gcgataccag ttccaggcca gcagcgaagt ggcggtgagg gccactacca ccacgccata
      661 ataaaagctg tgcgccgtca actcccaaac caccgcagtc gtgtaagcca gtgcggggaa
      721 accgaggacc gctccaagca ggagctgacg gcgacgggta ctcgcccgtg gcgacatgga
      781 gatcaggaaa gctatgcttg cgagttccaa agctcccagg ttaacggcgg atgccacatg
      841 gtcccaagga ccgtgtccta catctacaaa cttggcgacg aagtgtatga gtacgaggat
      901 ccaggccgtc agccatagct gcagccgctc ggccgcaccc tgacgcagaa tggcccggaa
      961 caccacgacc agaattgcaa gtgcaactag actgggcaga acgttaaagt tcatcacgta
     1021 agctttggcg ctgacctagg gctcttcaga gtatgacagt gattggcgag gaattctaag
     1081 gaaaaatcag gggggtggcc ggaacttaag caaatgcgtt tccaaaaggg aggttcccat
     1141 attagtcgta tatcggattg ctcaaccttc tgctgggcca gtgacgcgag ttttaggtag
     1201 acccgagaag ccgtcatcca cagcgaaagc gaaggacgat tgtttggctg gggcagctct
     1261 gatcctgagt tttacatggg cctgcggcgc acccatagga atgaaaattc accacggagg
     1321 cacggagatc acagagaaaa ctcgatagaa accaaaactc cgtgtctccg tgtttccgtg
     1381 gtaaatgatt ttcgacgagc gaggatgcgt gtccgagtga atccgaaatt gtactccaac
     1441 tgttcagtgc actttgacgt cgggcccgac tgacatgccc ggacgcagca ggccatcctt
     1501 gttgaagtcg ggcttgtcgt tgccatcgaa gtcgattctc actggaatac gctgcacgac
     1561 cttaacgtag ttgccggtcg cgttctccgg tggtaacaag ctgtactttg cacccgtcgc
     1621 tcctccaatg ctggtaacgt gcccgtccca cttgcgaccg ccgtaggtgt caaccttgat
     1681 cttcgcgggt tgccctggcc tcatgtgggc gagctgggtt tccttgaagt tggctgtgac
     1741 ccagatatcg ctaaggggaa cgatggccat caggtcctgg ccgctggata tgttcgctcc
     1801 tacttgcacg gagcgcttgc ctaccacgcc atctaccgga gccacgatgg tcgcgtacgc
     1861 gagattgagc tgcgcttgat cgagcgcggt tttggaccgt tgcgccagtg catcggcgga
     1921 cttcgccttg gctcgggtgg cggccaccat gttcggagtc gcccgcgcgt tacgcacttc
     1981 ggcggtggcc tgttcgatac gggattgtgc ttgctttact tgcgcctggg cggccagtaa
     2041 tgccgcctga cgcgcgctga ccgtagcatt ggcggctgca gctgcggccg tagcttgatc
     2101 gaactgctgc tgcgagatct cgcgcttgcc caacagttgg cgataccgct caacatcggt
     2161 attgagtttg tgggcattcg cttgcgcttc cagtatctgg gcttgtgcag cctctgcctg
     2221 tttctgggcg gcggtcacgc cggcttgcgc ggag
//
LOCUS       DASRKK010000316         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1001543, whole genome shotgun sequence.
ACCESSION   DASRKK010000316 DASRKK010000000
VERSION     DASRKK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001543"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1650
                     /locus_tag="VFC15_06800"
     CDS             292..1650
                     /locus_tag="VFC15_06800"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZM09902.1"
                     /translation="MTYQSTVAQLYALGHELASTPAHKFDLTYVREMLDALGHPERRF
                     ASVLIAGTNGKGSTAATLSSILRVAGYKTALYSSPHLVRINERIRVNGEAISDAEFGE
                     MHDRVERTAQGLLRFGKLPWHPSFFEMLTVMAFEYFASVGVEIAVLEVGMGGRLDATN
                     VVEPMISVIADIALDHQKYLGDTITEIACEKAGIIRQNGIVVTLPQHPQANDVIGHAI
                     LEKNARGVSAVKYVLPVSPGAVAYLAGGAVRVPGPSRYPLIVMGEEIDVDSPLAGRHQ
                     LRNLALAIATAEELAEFGFKVTPKQMERGIRETHWPGRFQVLPPSAATHHREMVLDVA
                     HNPAGAWALRSALSENFPGRELTLVFAAMRDKAVQEMAEILFPIAEQAIVTKVANPRA
                     ATTSELLQAASRTGTRLCEEQTVATALARAREISKPQGVIVVTGSIFLVGEAMSAMGI
                     SA"
     gene            1655..2491
                     /locus_tag="VFC15_06805"
     CDS             1655..2491
                     /locus_tag="VFC15_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM09903.1"
                     /translation="MTNTGPQVASRESGRGSLLSRLRSYLFFAPMVYLYTGAMGTLSL
                     LSSLSDREGRIQHWFARTWARMILKTVMIRVRVEGLDHINPVQTAIYAANHLSALDIP
                     VLYAWLPVQFRIMAKKELFRYPFLGWHLKRSGQIPIDAGDARASLRSLTHASESLRHG
                     TPLAVFPEGGRSATGHLQKFMGGAAYVAIRAQAPIVPIAIVGTNEVLPMNSFHLLPGV
                     VELIIGEPIPTTGLRVRDMDRLTMQVRRAIAEMFNSRSKTASLPETSGEAKPKPLLTP
                     NS"
BASE COUNT          500 a          767 c          798 g          515 t
ORIGIN      
        1 ggcggcggcg atcaagttcg agactgagtt caatcgatca ataaagcaga tccgggagac
       61 tccctggcgc tgggctcctt atctgaggga ttgccgacgg ttcctacttc accagtttcc
      121 attcgcaatt gtctatcaag ataccccaac cgttattcgt gtcgtagccg tcgctcatgg
      181 ccatcggaga cctggctact ggaaagatcg gacctgagcg ccgtcactca atgggttcgt
      241 cttctggaga cgtttctgaa cttccgaatg ttgtaatctg aaactgcccg catgacctac
      301 caaagcaccg tagcgcagct ctacgcgctg ggacacgagc tcgccagcac gcccgcgcac
      361 aagtttgact tgacctatgt gcgcgagatg ctggacgccc tcggccatcc cgagcggcgt
      421 ttcgccagcg tgctcattgc ggggacgaac ggcaaaggtt cgaccgcggc aacgctctcg
      481 tcgattttgc gcgttgccgg atacaagact gcactctaca gttcgccgca cctggtccgc
      541 atcaacgaac ggattcgcgt caacggcgaa gccatctccg acgccgagtt cggcgagatg
      601 cacgaccgtg tcgagcgcac tgcgcaaggg ctgctgcgtt tcggcaagtt accctggcat
      661 cccagcttct tcgagatgct gacggtgatg gcgtttgaat atttcgccag tgtcggcgtg
      721 gagatcgcgg tgctggaagt cggcatgggc ggacgactcg acgcaaccaa cgtcgtcgaa
      781 ccgatgattt cggtcattgc cgatatcgcg ctcgatcatc agaaatatct cggcgacacg
      841 attaccgaaa tcgcgtgcga gaaggcgggc atcatccggc agaatggcat tgtggtcacg
      901 ctgccgcaac atccccaggc caacgatgtg atcggccacg ccatcctgga gaagaatgcc
      961 cgtggcgtga gcgccgtgaa atatgtgcta ccggtgtcgc ccggcgctgt ggcgtatctc
     1021 gccggaggcg cggttcgtgt tcccggcccc agccgttacc cgctcattgt gatgggcgaa
     1081 gagattgacg tggactcgcc gctggccgga cggcatcaac ttcgcaacct ggcgttggcg
     1141 atcgccactg cggaagaatt ggcggagttc gggttcaaag tgacgccgaa gcaaatggag
     1201 cggggaattc gcgagaccca ttggcctggc cgcttccagg tgctgccacc gtcggcagcg
     1261 acccatcacc gcgagatggt gctcgatgtg gcgcacaatc cggcaggcgc ctgggctttg
     1321 cgctctgcgc tttcggagaa ttttcccgga cgcgagttga ccctggtctt cgccgccatg
     1381 cgcgacaagg cggtgcagga gatggcagag atactgtttc ccattgccga gcaggcgatc
     1441 gtgacgaaag ttgccaaccc gcgagcggct actaccagcg aactgctgca agccgcctcg
     1501 cgaactggga cgcggctatg cgaggagcag accgtcgcta ccgcgctggc gcgtgcgcgc
     1561 gagatcagta agccgcaagg cgtgattgtg gtgacggggt ccatcttcct ggtcggtgaa
     1621 gcgatgtccg cgatgggtat ttcggcatga cgcgatgacg aacactggac cacaagtcgc
     1681 aagccgcgaa tcgggccggg gctcgctgct gagccgtctg cgctcgtact tgtttttcgc
     1741 gccgatggtc tatctgtaca cgggagcgat ggggacactt tcgttgctct cgtcgctgtc
     1801 cgaccgcgag gggcgcatac agcactggtt cgcgcgtacc tgggcgcgca tgatcctgaa
     1861 gacggtcatg attcgcgtgc gtgtcgaagg gctcgaccat atcaacccgg tgcagacggc
     1921 catttatgct gccaaccatc tgtcggcgtt ggacattccc gtgctctacg cgtggctccc
     1981 ggtgcagttc cgtatcatgg cgaagaagga gctgttccgt tatccgtttc taggctggca
     2041 cctaaagcgg tcagggcaga tcccgattga tgcgggcgat gcgcgcgctt cactgcgtag
     2101 cctgacccat gcaagcgagt ccttgcgcca cggcaccccg ctggctgtgt ttcccgaagg
     2161 cgggcgctcc gccacgggtc acctccagaa atttatgggc ggcgcggctt atgttgcgat
     2221 cagggcgcaa gcgccgatcg tgccgatcgc aatcgtggga acgaacgaag tgctgcccat
     2281 gaatagcttc catctgttgc caggagtggt ggagttgatc atcggggaac cgatccccac
     2341 caccgggctg cgcgtgcgtg acatggacag gctcacgatg caagtacgac gggcgatcgc
     2401 tgagatgttc aattcgcgct cgaagaccgc gtctttaccg gaaaccagcg gcgaagcaaa
     2461 gcccaagccg ctgctgacgc cgaactcgta gctccagcga aaagaagcga cgatatgaaa
     2521 ccgcgtattg ccattccaac tcccaactcg acgcggccgg actatgtgtc acgcgcgctg
//
LOCUS       DASRKK010000317         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_24020, whole genome shotgun sequence.
ACCESSION   DASRKK010000317 DASRKK010000000
VERSION     DASRKK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24020"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..815)
                     /locus_tag="VFC15_06810"
     CDS             complement(198..815)
                     /locus_tag="VFC15_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZM09904.1"
                     /translation="MPSAYTLAVAGCLSLSLARAQAPALRDAPITTFGVTVYDSSGLR
                     GEVYLIPPESPALPKFEKLKPVGTIYASALNVAPTDYQETLQPLTKRFEWFAIDYTGN
                     FYISDPGKYRFLIASDDGSKLYIDDKLIIDNDGLHILKPEEGKLTLKGGIHRIRVSYF
                     QGPCCGMALILAVARPGEDWRLFSTREFGPPRTPAEWKYGAPDKK"
     gene            complement(1142..2686)
                     /locus_tag="VFC15_06815"
     CDS             complement(1142..2686)
                     /locus_tag="VFC15_06815"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM09905.1"
                     /translation="MADQQILVEIPEQAERKAKPQHGELKLKSVNRQQTLMANIYVED
                     LISADHKARAIEALVMKFDLSQFTESLKTTQGCVGRAAWHPALLVSLWVYAYSEGITS
                     AREIERLMPWEPGMQWLSGMETVNAHTLADFRVEHKSALDELFAELLALLEEGGLVNL
                     EQVMHDGTKIRAQGGGDSFRREKTLRERLADARAAVKEMGEAQGETNAKDRKQAAQQR
                     AARERMKQLESALEELAVLQAEKKEAGEKDKVRVSLSEPQARNMKHGDNAIAPSYNAQ
                     ITTEAEHKIIVGAHVSQCSSDAHSLMPALEEATQNLGKSPVQVVVDGGFTNRNNIIEC
                     ARQQIDLVGSLADPKERSEAAMKAQGIDPDFAPHHFRILEDGQRLQCPAGCSLEAVRQ
                     SVKRGDRYQQYQARGEDCRGCAYQTRCCPKSREQGRTVSIRKEEQAEVAAFRKKMETE
                     ESKAIYRRRGEVAEFPNAWIKEKLGLRKFRVRGLVKAESELLWACLTYNIMQWIRLVW
                     RQPAIV"
BASE COUNT          503 a          917 c          756 g          686 t
ORIGIN      
        1 caccgttgca gcacccgtac gaatacctgc tcggtaatta ccgattcaca caatgcgctc
       61 ctgattcccg aaagtcaatg ccttcactga atcttcatgc tttttagccc taacggtcgc
      121 ggctctgcac gcgcatcgga agctgagacc gtcagggagc ggttggcatg gttatttcgg
      181 aacgggcgct aattcgccta cttcttgtcc ggggcgccgt acttccactc ggccggggtg
      241 cgcggcggac cgaattccct ggtgctgaag agccgccagt cctcgccggg acgcgccacc
      301 gcgagaatca gcgccatgcc gcagcagggt ccctgaaaat aggagacgcg gatgcgatgg
      361 attccgcctt tcagtgtgag tttgccttct tccggtttca ggatgtgcag gccgtcgtta
      421 tcgatgatca gtttgtcgtc gatgtagagt ttagatccat cgtcggaggc aatcaggaaa
      481 cggtacttgc cgggatcgct gatatagaaa ttgccggtgt aatcgatagc gaaccactcg
      541 aagcgcttgg tcaggggctg cagggtttcc tggtaatcgg tgggggcaac gttgagagcg
      601 ctggcgtaaa tcgtgccgac aggtttcagc ttctcgaact tgggaagagc gggactctcc
      661 gggggaatca ggtagacctc gccgcggagc ccggaggagt cgtaaacggt gacgccgaag
      721 gtggtgatgg gcgcgtcccg cagggcgggg gcttgcgcgc gcgcaaggga gagggacagg
      781 cagcccgcga cagctagcgt atacgcactg ggcactctcc tatgatactc acaggtgata
      841 cttagggctc gttctgaaat aaccgtgcca accgctccct ggtcgcggct ccgatgcgcg
      901 ttcagagccg cgaccgttag aagctgagaa atattgtggg cgataacgcc gaatcatgtt
      961 tccagctcga aaaatgattg ttactttaat taagtcgttt cactgcggcc cttactctga
     1021 cccagttccc gaactttggt tttgcaccaa acgtcattaa ctctttgatt ctaagtgtgt
     1081 attctcccgg ccccgtcgag tgtgctcgcc gcggcgcttt ccgccatcct tcgttgtcgc
     1141 cttacacgat cgccggttgg cgccagacca gccggatcca ttgcatgatg ttgtacgtca
     1201 gacacgccca cagcaactcg ctctccgctt tgaccaaccc ccgcacccgg aacttgcgca
     1261 gtcccagttt ctccttgatc catgcgttgg ggaactccgc cacctctcca cggcgacggt
     1321 agatcgcctt gctctcctcg gtctccattt tcttgcgaaa cgccgccacc tctgcctgct
     1381 cttccttccg tatcgaaacc gtccgccctt gctcgcgact cttgggacag caccgagttt
     1441 gataggcaca gcctcggcaa tcttcccctc gtgcctgata ttgttggtac cgatcccctc
     1501 gcttcacact ctgccgcacc gcctccaaac tacatcctgc cgggcattgc agccgctgcc
     1561 cgtcttccag aatgcggaag tggtgtgggg caaagtctgg gtcgatgcct tgcgccttca
     1621 tcgccgcctc acttctctcc ttgggatccg ccagcgatcc caccagatcg atctgttgcc
     1681 tcgcacactc gatgatgttg ttgcgattcg tgaatccgcc gtccaccacg acctgcacgg
     1741 gactcttccc cagattctgc gtcgcttcct ccagcgccgg catcaggctg tgagcatcgc
     1801 tgctgcattg gctcacatgc gccccgacga tgatcttgtg ctctgcttcg gtggtaattt
     1861 gcgcgttata gctcggtgcg atggcgttgt ctccatgttt catgttgcgc gcttgcggtt
     1921 cgctcaggct gactcgtacc ttgtcttttt cgcctgcctc ctttttctcc gcctgcagca
     1981 ccgccaactc ttccagggcc gactccaact gcttcatccg ttcccgcgct gcccgctgct
     2041 gtgccgcctg cttgcggtcc ttcgcgttag tctcgccctg cgcctctccc atctccttca
     2101 cggccgcccg cgcatctgcc aaccgctccc gcaacgtctt ctcccgtcgg aagctatccc
     2161 cacccccctg cgcccggatc ttcgtcccgt catgcatcac ctgctccagg ttcaccagcc
     2221 ctccctcctc cagcagtgcc agcaactcgg caaacaactc atccaaagcg ctcttatgct
     2281 ccacccggaa atccgccagc gtatgcgcgt tgaccgtctc cataccactg agccactgca
     2341 tgcccggctc ccagggcatc aagcgttcga tctcgcgtgc cgaggtgatt ccttcgctgt
     2401 acgcatacac ccacagactg accaatagtg ccgggtgcca ggcggcccga ccgacgcagc
     2461 cttgggtagt ctttaaggac tctgtgaact gcgacagatc gaacttcatc accagggctt
     2521 cgatggcgcg cgccttgtga tccgcagaga tcaagtcttc aacgtagata ttcgccatca
     2581 aggtctgttg gcggttgacc gacttcagct tcaattcgcc atgctggggc ttagcttttc
     2641 gctcagcttg ctcggggatc tccaccagga tttgctggtc tgccatgggc atgcatcaat
     2701 tatcggacaa gtacttgtct aatgaaagta ctttgtattc atatttgaat ttttctcggc
     2761 ttctcaggga gcggttaccc atgctcaagc gtagatgccg cgcaggcgga tggccagggc
     2821 cacccggtcc agcgccagca tgtaggccgc cgtgcgattg tt
//
LOCUS       DASRKK010000318         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_317075, whole genome shotgun sequence.
ACCESSION   DASRKK010000318 DASRKK010000000
VERSION     DASRKK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317075"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(606..1847)
                     /locus_tag="VFC15_06820"
     CDS             complement(606..1847)
                     /locus_tag="VFC15_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZM09906.1"
                     /translation="MGEPAFRLASALAIGLLIGAERERSKGEGPLRSAAGIRTFALAS
                     LLGGVSVVLGGNLLLAVTTGVIAAFCIVAYLRTHEQDPGLTSEAALVLTALLGGLAQR
                     QTATASALAVVVTVVLAARSRLHHFVSRVMSEEELTDALIFAAVILVVLPLMPNRYLG
                     PFGAINPHTIWKIVILMMSISAGGYIAVRLLGPRFGLPIAGLASGFVSSTATIGAMGA
                     LALREPALARSAVAGAVLSTVATILQMTAVLAATNRATLYEMRFPLVTAGIAALVYGA
                     ILTFLSVRQKTAPPAQKGRAFSLKSAVFFAGMIAVMLLLSAALNSQLGKAGVLAAAAV
                     AGFADTHSAAVSVASLVAAGRFAAQDAVIPILAGLTTNTIAKAAVAISTGGRRFAVQI
                     IPGLVIVIAAAWVAAGFALRH"
     gene            complement(2804..3298)
                     /locus_tag="VFC15_06825"
     CDS             complement(2804..3298)
                     /locus_tag="VFC15_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09907.1"
                     /translation="MARRILSQEDAARIIELSDTACIREIAKRFKTTTRTIYLKIYNH
                     HKSAGHSGMIYTVSAITNLLKVTPATVKQWGAEGRLEIQHQQRGSVTAYIVEDDEFER
                     FCRDNLNYLIFMVGGRVAPRERILFLKQFVIAADMPDDHTARSHRREREAYAELTDSG
                     DDLD"
BASE COUNT          779 a         1009 c         1024 g          748 t
ORIGIN      
        1 cgctgtcgag gatcgtggct agcgcactcg tacgggcagc cgtgggcaac tgcaagaaaa
       61 ctgccactcg cggatcgctc agcgacttgt cgatcaagtg atcgagcaaa gaacaatttc
      121 ggccgtcgcg attgcgatga gcgctaggga cggtcgatcc ttgcttcatg caagtagcat
      181 ccgccagtct gcatgccttt gtcagtgatg tggcgtgaag taacaaaaat agcgctcggc
      241 ccctaacgat tgcctcccgc tgttatgtca cgccagagac atgcgctgac agacaagaat
      301 ccttgatcta tggaggcgtt gaacagtaac tcgcgaagag tcttagggct gtatcggaag
      361 cgcccctacg aggaatcctg gatttcattg gagtgtgcgg gcgctttcga tacggcccta
      421 ggacttctgg ccggtgtcaa agggagccgc ccagacctgt aaattcagag tcgggccgtg
      481 aagcgagaat attctctgaa aagccaagat tcccttgcgg gtcgaactct tctcaattat
      541 gcgagctaat gagaggccga tttttcatca attgccacga acgtggacga ggcaaggaat
      601 aggactcaat gtcttagcgc gaacccagca gccacccatg ccgcagcgat tacgatcacc
      661 agtcctggta tgatctgcac ggcgaagcgg cgccccccag tcgaaatcgc gacggctgcc
      721 ttggcaatgg tgttggtggt gagtcccgcg aggatcggta tcactgcatc ctgggcggcg
      781 aacctgccag ccgccactag ggaggcgacc gaaactgccg cagagtgcgt atcggcgaac
      841 cccgcaaccg ccgctgctgc cagtacgcca gccttaccta gctgcgaatt gagcgcagcg
      901 gacaacaaca acatgacagc gatcattcct gcaaaaaaga cggcgctctt taggctgaag
      961 gcccttcctt tttgcgcagg cggagcagtc ttttgccgca cgcttagaaa tgtcagaata
     1021 gcgccgtaga ctagcgctgc aattcccgcc gtgaccagag gaaagcgcat ctcgtaaaga
     1081 gtcgctcggt tggtggccgc cagtacggcg gtcatctgca ggattgtcgc gaccgtagac
     1141 aaaaccgctc ctgccactgc cgaccgcgcc agtgctggct ccctcagagc gagcgctccc
     1201 atcgctccaa tcgttgcggt gctcgatacg aaccccgaag ccagccccgc gatcggcagt
     1261 ccaaaacgcg ggcccaatag tcgcaccgcg atgtatccgc cggcactgat ggacatcatc
     1321 aggatcacga tcttccaaat ggtgtgcggg ttgatggcgc cgaacggccc aagataacgg
     1381 ttgggcatca gtggcaggac caccaaaatt acagccgcga agatcagtgc atcggtcaac
     1441 tcctcttcgc tcatcaccct gctaacaaag tggtgaaggc gactgcgagc cgccaacacc
     1501 acggtcacga cgaccgcgag ggccgaagca gttgccgtct gcctctgtgc tagtccaccc
     1561 aacaacgcgg tcagcaccag cgcagcttcg gaagttagtc ccggatcctg ttcgtgcgtc
     1621 ctcaggtagg ccacgatgca aaacgcggcg ataacccctg tcgttactgc cagcagcagg
     1681 ttgcccccca aaacgacact cacgccgccc agcagtgacg ccagcgcgaa tgtccggatc
     1741 cctgcggcag aacgcaacgg gccttctccc ttgctccgtt cacgctcagc gccaatcaaa
     1801 aggccaatag ccagcgcact ggccaaacgg aatgcgggtt cgcccatgga aaacatgcgt
     1861 ggagatacta tcaccgctgc gtgtccgaac gctgtggcaa gtccgagtct tacccccggt
     1921 atcaagctga atgcgcttaa acacaggaca gttacagttt cctcggaaaa tcacttgatg
     1981 aaaacaagct tcgcgtgagc tgaggtctaa tgacgggtat taccgggacg gtcgttcatg
     2041 caggcacttt ccacatacaa gagggagacc gctgctttat gcggtgcgtt aggccatcgt
     2101 gacaagtcat tgctcgaaga tatcgccagc cgtgcgactg ttttttcgtg gagagaattg
     2161 ttgggtttgc ttcccgggcg accggacgat ccggatgccc aacgagtact caacgagctg
     2221 aaccggcgta tctgaatccg cctgcgttca ctcgcatgac atcaagatga aggcggcttc
     2281 ggttctgcaa tcgatgccag ctcgcgaaga atttggaagg tcttgtgcac aagatgccga
     2341 ctgaggaaga gttcttagcg cgcatatcgg tgaatcgcac ctggcgcatt caagcgtgag
     2401 cgatctcaga agatgcgcga agtccagcca gaagagcgga gaggcggaat ccgagttcga
     2461 cgatctctga cgtcgtgcta tgaccgcgcg ggaacagcaa tgcatgaatt gctcgaaaca
     2521 tccgagaccg cggcaatccg ctatgctggc gtggacagaa acacctccgc gctggaagaa
     2581 gtgtggcggt tagtcgtcat attgtggatg aagcggccga gtacagatga tctgcattcg
     2641 gccgctgaga gtggcggcct gccgttagtt ggtcttgcta aactcggctc aacgcgtgcg
     2701 gggttcacag cacattcgct ggctggcaaa ccttgtcggt ttgccaattt gtctgcaacg
     2761 ttctggcgcg gcagagatct cggatctgtc aggcagatcg catttagtcg aggtcgtccc
     2821 cggaatcggt tagctcggcg taggcctccc gttcacgccg gtggctgcgc gccgtgtgat
     2881 catcgggcat gtcagctgcg atgacaaact gtttcaggaa gaggatgcgc tcgcgcggag
     2941 ccacgcgccc tccaaccatg aagatcaggt aattcagatt gtcccggcag aagcgttcga
     3001 attcatcgtc ctcgacgatg taggcggtga cgctgccccg ttgctgatgt tggatttcca
     3061 gcctgccttc cgcaccccac tgtttcaccg tggctggggt aactttcagc agattggtga
     3121 tggccgacac ggtgtagatc attccactgt gtccggcgct cttgtggtgg ttgtagatct
     3181 tcagataaat cgtgcgcgtc gttgtcttga accgcttggc gatttcccgg atgcaagcgg
     3241 tgtcggacag ttcgatgatt ctggctgcgt cttcctggct caagatccga cgtgccatcg
     3301 gccggcggaa gccaaatcgt tccgccaggc gccagcacgc tttctctgga tacgtcgtta
     3361 atgcatggag ttgctgcaca accaatacgc cgtccttctt gggtattctg gttgaccgga
     3421 cgatgcttaa tgctttctcc gaccagacat aatgctggcg gggcgatcgt gagcgggggt
     3481 tgtcgaatac catgggcctg acttacgaat cagattcgta tcctttcgct gaattgagaa
     3541 acgatgcttc aggcgtgctt
//
LOCUS       DASRKK010000319         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_218809, whole genome shotgun sequence.
ACCESSION   DASRKK010000319 DASRKK010000000
VERSION     DASRKK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218809"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFC15_06830"
     CDS             complement(<1..338)
                     /locus_tag="VFC15_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZM09908.1"
                     /translation="MPSLNLKGLAEGMTPKCESQASVAWAAQSGAMPVHAYDQMETLC
                     GAGSVGLEFEAQAPGIDTLLVACGGGGLIGGIAGWYQGRVRIVGVEPEKAPTLYRALE
                     AGRPVDAEAGG"
     gene            complement(416..2176)
                     /locus_tag="VFC15_06835"
                     /pseudo
     CDS             complement(416..2176)
                     /locus_tag="VFC15_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            complement(2256..2717)
                     /locus_tag="VFC15_06840"
     CDS             complement(2256..2717)
                     /locus_tag="VFC15_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM09909.1"
                     /translation="MSFHSRHLPHWQPEGAPLFITWCLHGSVPRNRFPPPGLTAGHAF
                     VWMDRYLDQATFGPTWLKREDIAQMVFDCLHYAADNLRHFDLHAGVVMPNHVHVLLTP
                     IITASKLLQSVKGFSAREANKILGRTGEPFWQAESYPDFRTSLPLSGCAEA"
     gene            2835..3299
                     /locus_tag="VFC15_06845"
     CDS             2835..3299
                     /locus_tag="VFC15_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZM09910.1"
                     /translation="MTRMENIRDFLAQKRFAFIGVSRQPKDFSRALFREFKTRDYEPV
                     PVHPEAAEIEGARCFAHLRDIQPPVESVLLMTSPTVTNLLVRECVEAGIKRVWFYRGA
                     SQGALTDSALQICEANGIRAIAGECPYMFFDGTPWFHRFHGLVKKIVGAYPR"
     gene            3621..3815
                     /locus_tag="VFC15_06850"
     CDS             3621..3815
                     /locus_tag="VFC15_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09911.1"
                     /translation="MKRRFRKILLLLVLWVGSQMGLPMRPEEIEELMRTMNEPKIVRK
                     FAEEEERGDDPLPTAGSRIS"
BASE COUNT          741 a         1201 c         1114 g          822 t
ORIGIN      
        1 ccgcccgcct cggcatccac cggccgcccg gcctccagcg cgcgatacag cgtcggcgcc
       61 ttctccggct ccacgccgac aatccgcacg cgcccctgat accacccggc gatccccccg
      121 atcaatccgc cgccaccgca ggccaccagc agcgtgtcga ttcccggagc ctgtgcctcg
      181 aactccagcc cgaccgaccc cgcgccgcac agcgtttcca tctggtcgta agcatgtacc
      241 ggcatggcgc cggactgcgc cgcccatgca acactagcct gactttcgca cttcggggtc
      301 atcccttcgg ctaacccctt cagattcagt gagggcactg caaaggtctt caccacaggg
      361 agggctgatc ggcggcctcg ggcggggcca ataataaggc cacctgcgcg gccttgatgc
      421 gtggcggtgg ttgggagggg agcgtgatct ggatgtggtc caacatcgcc tgcacgtctt
      481 tctctggctg ggtgtggcgc ggcaatatca gcgagcggcc gtcaagcatg ggaatccata
      541 cctctaccat ctggatcgtc gccagctttt cgaataccgc cgccggcgtt agccccggcg
      601 cgtgaatcat caggcggttc ttcaaagtaa cttgcaggca gtaggccagg aacgcaatca
      661 ggatgtgcgc gtcggcccga tgttcgagct gatgatagat gggacggaca cccaactcgc
      721 tcttcaacga gcgaaagaca ctctcgatct gcgtcagttg cacatagcgc gtccacaact
      781 ctgccggatc ttcggcggtg agattgctgc gcagcagata atggccgtcg cgctgttcgg
      841 acgctttcag cttggccttg tcgacctgaa aagagaaggt ctcgcgtgtc acctcctgat
      901 ccttggtggg caactgaatc ttcacgaacc cgaacgcccg gccggcttct tttctggcgg
      961 cgccgatacg taacagtaac tggtcgcgtt tgggcaagct cttgcgcatc gcgcgaagct
     1021 tgcgcaacaa gcggaccaat cgcttgcgcc gcatcgcgat ctccttcgcc tgccgcccat
     1081 cgctcttggc gagcacgtag agttcgccct catgctgata cagcttcacc tccaccgagt
     1141 cgcgcacctt ttgccacggc agatccagcc atttcttttc atgctcactg attctgctcc
     1201 tgggcgtgcc caccagatag aacgtctgtc gttcgggctc gcgcatctct ttcagaatcg
     1261 cctcggttgg aactccccga tccatcaccc atacccgccg cgccttgccg tacgtgccct
     1321 cgatcttctt gagaaagtcc ggcagcgtct tgcggtccga ggtgttgccg ttcatcactt
     1381 cataggccaa gggaaatcca tccggcgtga cgaccagggc aatcaccaat tgcagacaat
     1441 ccggcctctt atcccggctg tagccgcgct tggcttttgc attctgctcc atctcccctt
     1501 cgaagtacgt gctggtcaga tcgtaaagca gcacttcgaa atccgcgtgg aacaggtctg
     1561 cccatttctg ctttagccag atgaataact cctgtttgtg tttcagcacc cgatctaggc
     1621 agcggtacag gcgatccttc tcggccacgg aaaagtcggt ttccagcagt tcgtccatgg
     1681 cgctgtccac gaaccactgc cggtgcaggt gatactcgct gcctggatcc aacagccgat
     1741 tcaccaccag tagccgcagc actttttccc aactcaccct ctcgcgcgct tccggcaatc
     1801 gctgctgcca aaactcatcc agcccgagtt gctgccacag ctcgcaagcc agccagcagt
     1861 tgccgaatac ccgcggtcgt cgcagctcca acccgctcag cttcacctgg acgctgtcca
     1921 gcgcgtcggc gggaatctcc cggtcttcgg gaaacaaact catgctggtg tagcattgtt
     1981 cgtcttcatc gaacacatcc agtgtcttgc gccacgcagc ctgttgctgg tcgttgatct
     2041 cgcccaaata cagaaccgtc cgctgcaccg tcttgtcgcc tggcagtcgc cgattctcca
     2101 cgatgctgaa gtagcggtgg ttcttgccgt ccttcctgcg gttggtgctg cgtagaaaca
     2161 tcggaagcag cagcatgcca gaaatccaag cgtatgaaaa gggggtaggt cttcacccag
     2221 gggttttctg aaaatttacg atgtagacaa tagacttacg cctcggcgca ccccgaaagt
     2281 ggcagggaag tgcgaaagtc aggataggat tcggcttgcc agaagggctc gccggttcgc
     2341 ccgaggatct tgttcgcttc cctcgccgaa aatcccttca ccgattgcag caacttgctg
     2401 gctgtaatga tcggcgtcag gagcacatga acgtgattgg gcataaccac gccggcgtgc
     2461 aggtcgaagt gccggagatt gtcggcggcg taatgcaggc aatcgaagac catttgggca
     2521 atgtcctcac gcttgagcca ggtggggccg aaggtagctt gatccagata gcgatccatc
     2581 cacacgaagg cgtggccggc ggtgaggcct gggggcggga agcggttgcg gggaacactg
     2641 ccgtgcaggc accaggtgat gaagagtggg gctccctccg gttgccagtg agggaggtgg
     2701 cgggagtgga aggacatatg agttggtggc agggaggagg ggagattctc aagaaatgca
     2761 agaatgcccc caggaatggg ggcatagcag cctaaaaggc tacgctacaa tggacagtga
     2821 tattgagatc cggtatgacg cgtatggaaa acattcggga cttcctggca caaaagcgct
     2881 tcgcatttat cggcgtctcc cgccaaccga aagacttctc gcgagcgctc ttccgcgaat
     2941 tcaagacacg ggattacgag ccggtgccgg tccaccccga agccgcggag atcgagggcg
     3001 cccgctgttt cgcccatttg cgggacatcc agccacccgt ggaaagcgtc ctgttgatga
     3061 catccccgac cgtgaccaac ctgctggtcc gggagtgcgt ggaagcgggc atcaagcggg
     3121 tctggttcta ccgcggcgcc agtcagggcg cgcttaccga cagcgcgcta cagatctgtg
     3181 aggccaacgg gatccgcgcg atcgcaggcg agtgccccta tatgttcttt gacggcacac
     3241 cctggtttca ccgatttcac ggactggtga aaaagattgt gggcgcgtat ccgcgctgac
     3301 atacgacgct gacatacgct ctcgcggtac tatcaaggca ggactgtaga tgaattcccc
     3361 gcggaaactg ctcgcgctgc tgatggtcgc cgccctgctt tgtgcggtgt tcgcgcccgc
     3421 gtctttcgcg caatccgccg cagttctggt tccgctttgc ctcttctgtg tttgcctgac
     3481 gacggtttcg gttcgccgcg ttttcgaacg gtgtagcctt ccgtcatcgc cgctctgccc
     3541 gctgctcgct tcgcgggctc ctcccacccg gtagtccact gctgttgcac cgtccaccgg
     3601 ttccggaagg agagtctcgc atgaagcggc gtttccgaaa aatattgcta cttttggtgt
     3661 tatgggtggg ttcccagatg gggctgccca tgcgccccga ggaaatcgag gagttgatgc
     3721 gcacgatgaa cgagccgaag atcgtacgca agtttgccga ggaggaagag cggggcgacg
     3781 atccgctgcc taccgctgga tctcgtattt cttgatcttc tcgtagagcg tggagcggtc
     3841 gatccccaga accgaagcag cctctttgat attgccct
//
LOCUS       DASRKK010000320         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_708464, whole genome shotgun sequence.
ACCESSION   DASRKK010000320 DASRKK010000000
VERSION     DASRKK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708464"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFC15_06855"
     CDS             <1..231
                     /locus_tag="VFC15_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09912.1"
                     /translation="NAYRESLNGDGEPRWTEVWSHIQLGKIFDLTGQRERATNEYRQA
                     LQTNDNTQNALDEARKYLQSPFQPPSPDKPGM"
     gene            complement(664..1917)
                     /locus_tag="VFC15_06860"
     CDS             complement(664..1917)
                     /locus_tag="VFC15_06860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZM09913.1"
                     /translation="MTIHPNVLDSGVHRESRLRKRIADEIRRDGPIPFSRYMEMCLYE
                     PDLGYYSRSREQFGKAGDFYTSSDVHAVFGRLLARQFEEMWRVLDSPRHIDVIELGPG
                     RGLFAGDVLDWSAKTFPEFSRALHYALVEQAAHLRDRLQERLAEHIAAERARVFESLE
                     AAASTVGKNIIVFGNEFFDALPVEVVDYRGGLRIGLENGRFVETFVPPTAAEVEFLDR
                     YGIHPEQGERVEATLVSLEWMGRIAEIFRSFGTADPSARTEVLGRDDSEKHVAAENSR
                     RGFVVLIDYGYTREEQLAGRHRDTIMSYRQHTASTSPYEAPGEQDITAHVNFTALRAR
                     GVGRGLDPMALLTQSQFLIGIGEETEFADAFQECKLPQERAKVALQLKHLITPTGMGE
                     TFQVLLMSAGVAKEKAATLSGLKLG"
     gene            complement(1914..2219)
                     /locus_tag="VFC15_06865"
     CDS             complement(1914..2219)
                     /locus_tag="VFC15_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09914.1"
                     /translation="MTSSRLERVQQQLRLYEHPLVEFDARTKGEAVEVIIRPRFTGPP
                     VHTYYFELHPRDLDDLQFEWTFQRQLFDCLHDYLVEMFVRTPQHRKENQQMEREGNL"
BASE COUNT          496 a          723 c          684 g          490 t
ORIGIN      
        1 aacgcctatc gcgaatcgct caacggcgat ggcgagccgc gctggaccga ggtttggagc
       61 cacatccagt taggcaagat cttcgacctg accgggcagc gcgagcgcgc aaccaacgag
      121 taccgccagg ccctgcagac caacgacaat actcagaacg cgctcgatga ggcgcgcaaa
      181 tatctacaat cgccattcca gccgccaagc cccgataagc ccggcatgta gaaggtgtca
      241 gctatctcat aatctggttt gaaagcacgc ggcgtcattc atgtcgctag agctattccg
      301 gatttggtgt acctcatcga acgacgaaga cttggattcg tgtcagggca cgacttcagt
      361 cgtgccgcaa gctttgagaa atgaatcggg ctttagcccc tgtagccgtg caccacaacc
      421 ccaggtgcta aagagcgcgg gtaagaactc cttttgagtt ggcacggtgg aacgattgcg
      481 caaaaatcca gcgccgtagc cggggtcccc aggtcgcgcc gcttttgcgc gaactggggt
      541 ggaggtaggc gcggatcagt ttagcccagc acagagccgc aggcggagtg ctgggtagag
      601 tggaaaatag atatgagtcc cttcagggac ggcacagctc ttccacgcct gtgaagtccg
      661 gcactacccc agtttcaggc cgctcaatgt agcggccttt tctttcgcta ctcccgctga
      721 catcaggaga acctgaaacg tctctcccat gcccgtgggc gtgatcaagt gtttcagttg
      781 caatgccacc ttggcgcgct cctggggaag tttgcactcc tgaaacgcgt ccgcaaattc
      841 cgtctcctct ccaatcccga tcaaaaattg cgattgggtg agcagcgcca tcggatcgag
      901 tccgcgccca acgcctcttg ctcgcaatgc cgtgaagttc acatgggcgg tgatgtcctg
      961 ctcgcctggc gcttcgtagg gcgacgtgct ggcggtatgc tgacggtaac tcattatggt
     1021 gtcgcgatgg cgtcccgcca gttgctcctc gcgtgtgtag ccgtagtcga tcaggaccac
     1081 aaacccgcgt ctgctgtttt ctgcggcgac atgtttctcg ctgtcatccc gaccgaggac
     1141 ttcagtccga gcggagggat ctgcggttcc aaacgagcgg aatatctctg cgatgcgccc
     1201 catccactct aatgacacta gcgtcgcttc cactcgctcg ccctgttccg gatgaatacc
     1261 gtaacgatcg aggaattcga cttccgctgc ggttggcgga acgaacgtct ccacgaacct
     1321 cccgttctcg agcccgatcc gcagcccgcc gcgatagtcc actacctcga cgggaagggc
     1381 atcgaagaac tcattgccga agacgatgat gtttttccct actgttgaag ccgccgcttc
     1441 gagcgactcg aagacgcgtg ctctttctgc cgcgatgtgc tcggctaatc gttcctgcaa
     1501 tcgatcgcgc aaatgcgccg cctgctccac cagcgcgtaa tgcagagcgc gcgaaaattc
     1561 cgggaacgtc ttcgccgacc aatccagcac atcgcccgca aacaggccgc gccccggacc
     1621 cagttcgata acgtcgatgt gccgcggaga atccagcacg cgccacatct cctcgaactg
     1681 ccgcgccagc aggcgcccaa acacggcatg aacgtcgctc gaggtgtaaa agtctccggc
     1741 cttgccgaac tgctcccgcg agcgcgagta gtacccgaga tcgggttcat agagacacat
     1801 ctccatgtag cgcgagaacg ggatggggcc atcgcggcgt atctcgtccg cgatgcgctt
     1861 gcgcaggcgc gattcacgat gaacgccact gtcaagcacg ttagggtgaa ttgtcacaga
     1921 ttgccttcgc gctccatctg ctgattctct ttgcggtgct gtggcgtgcg tacgaacatc
     1981 tcaaccaggt aatcgtgcag gcagtcgaag agttggcgct ggaaggtcca ctcgaattgc
     2041 agatcgtcga gatcgcgggg atgcaactcg aagtagtagg tgtggaccgg cgggccggta
     2101 aagcgtggcc ggatgatcac ctccaccgct tcgcctttgg tgcgcgcgtc gaactccacc
     2161 agcggatgct cgtacagccg caactgctgc tgcacgcgtt ccaagcgaga ggaggtcatc
     2221 ggccgattgt agcgggccgt caggttttca caaagaagtt acaatttcgc gattccacgg
     2281 tgacaactca aggctacgtc ccggctattc tttcgcctga agtgcaaggc ggatatggcg
     2341 gacggctgcc gtgagtaacc aatcacggag gtcgcccgcc atatgttttg ggt
//
LOCUS       DASRKK010000321         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1294545, whole genome shotgun sequence.
ACCESSION   DASRKK010000321 DASRKK010000000
VERSION     DASRKK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294545"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFC15_06870"
     CDS             complement(<1..1304)
                     /locus_tag="VFC15_06870"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZM09915.1"
                     /translation="MSSAAVSYARENQKRFLDELKDLLRIPSISTAEEHKDDVRKAAE
                     VVASDLKNIGFEHVETIATQGHPLVYADWLHASGKPTALCYAHFDVQPAEPLDEWNTP
                     PFEPTERNQNIYARGAVDDKGQLWMQLKAFESLFKTGDGKLPINVRVIFEGEEEVGGE
                     SIEEFVKKNASSGKLKADFALVTDTELFAPDLPTLCVGLRGLVYTEIEAQGAKSDLHS
                     GVYGGAAPNPLFALIEIISKLKDADGKILIPGFYDDVQKPTADELKAWDRLPFNEEDY
                     RKAEVGSSVLTGEPGYSVMYRTWARPTLEVHGMPGGFVAAGAKTVIPARASAKVSMRL
                     VPKQDPDDILKKYTEFVKKHTPKGIQINIKVHSKGPACVVNTDNKFARASVEAMHEIF
                     GKDTVYIRSGGSIPIVTQFEEDLKIPSIMMGMGLPDDNLHAP"
     gene            complement(1811..>2333)
                     /locus_tag="VFC15_06875"
     CDS             complement(1811..>2333)
                     /locus_tag="VFC15_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09916.1"
                     /translation="NNVGRGVVGAIRTGFNQVESGPVLVVMADLSDDLAQVDRMVEYY
                     RQGYQVVVGSRYMPGGRLLDAPPLKGAMSRVAGLTLHWFRGIPTHDATNAFKLYDSKI
                     LKHMTIESKAGFELSLEITVKAFLAGYRIVEVPTTWRGRTQGESRFKIWAWLPQYLKW
                     YFYAFQPKRRSVQ"
BASE COUNT          452 a          699 c          686 g          496 t
ORIGIN      
        1 ttgggggcgt gcaggttgtc gtcgggcaga cccatgccca tcatgatgct gggaatcttc
       61 aggtcttcct cgaactgggt gacgatggga atcgaaccgc ccgagcgaat gtacacggtg
      121 tccttcccga agatctcgtg catggcttcc accgacgctc gcgcgaactt gttgtcggta
      181 ttcaccacgc aggccgggcc cttgctgtgc accttgatgt tgatctgaat gcccttgggc
      241 gtgtgcttct tcacgaactc ggtgtacttc ttcaggatgt cgtccgggtc ctgcttgggc
      301 accagccgca tcgagacttt ggccgacgcg cgcgccggaa tcaccgtctt cgcgccggca
      361 gcgacgaagc cgccgggcat gccgtgcacc tccagcgtcg gacgcgccca ggtgcgatac
      421 atcaccgagt agcccggctc tccggtcagc acgctcgatc ccacttcagc cttgcgataa
      481 tcctcttcat tgaagggcag acggtcccag gctttcagtt cgtcggcggt cggcttctgc
      541 acgtcgtcgt agaagccggg aatgaggatc tttccgtccg catctttcag cttgctgatg
      601 atctcgatca gagcgaacag cgggttgggc gccgctccgc cgtacacgcc ggagtgcaga
      661 tcgctcttcg ctccctgcgc ttcgatctcg gtgtacacca agccgcgcag tccgacgcac
      721 agcgtaggaa gatcgggagc gaacagctcg gtgtcggtga ccagcgcgaa gtcggccttc
      781 agcttgcctg agcttgcgtt cttcttcacg aactcttcaa tgctctcgcc gcccacttct
      841 tcttcgccct caaagatgac gcgcacattg atgggcaact tgccgtcgcc cgtcttgaac
      901 agcgactcaa aagctttgag ctgcatccag agctggccct tgtcgtcaac ggcgccgcgc
      961 gcgtagatgt tctgattgcg ctcggtgggc tcgaacggcg gagtgttcca ctcgtccagc
     1021 ggctcggcgg gctgcacgtc gaagtgcgcg taacacagcg ccgtgggctt gccggaggcg
     1081 tgcagccagt ccgcataaac caagggatgg ccttgcgtgg caatggtctc gacatgctcg
     1141 aagccgatgt tcttcaggtc gctggccacc acttcggcag ctttgcggac atcgtctttg
     1201 tgctcttcgg cagtgctgat gctgggaatg cgaagcaaat ctttcaactc gtcgagaaac
     1261 cgtttctggt tctcgcgcgc gtaggaaact gctgcggatg acatagggaa aagttctcct
     1321 tccagaactg gacagcttgc gtgctactgc tggcaaacat tgataggcaa accagaaagt
     1381 atatcgcaac cgaggcgggg agttgtgacg cagatgcggc gagctgtacg gtcacgctgc
     1441 gctgagaggg cggtcgcaac cccgcgctgg aatatccagt acgcgagtcc cgcaaggcgg
     1501 gacgaaagaa cttagcccag tgcttcagct ggattattca tgaggacttc acgctttgaa
     1561 ggggcacggc ttcagccatg ccgtaagcgc tgtcattatg gtggctttag ccactgaggg
     1621 acttcggggt tgagacgaag tgcgagttcc ctcaggagct aaagcccaac tctccacgca
     1681 cacgtaatgg cacggctgaa gaggctgctg aaaaggtcga aaaagcagat ccctcgcggg
     1741 ctgaagcccg ctcgtgatga caagaaataa taaagggctg gacgcggacc tgaaggtccg
     1801 ctctactcaa tcattgcacg gagcggcgct tgggttggaa ggcgtagaag taccatttca
     1861 aatactgcgg caaccacgcc catatcttga accgcgactc gccctgcgtt ctgccgcgcc
     1921 aggttgtcgg tacctccaca atgcgatagc cggcgaggaa agctttcacc gtaatttcga
     1981 ggctgagttc aaagcccgcc ttgctctcaa tcgtcatgtg cttcaggatt ttgctgtcgt
     2041 agagcttgaa cgcattggta gcgtcgtggg tgggaattcc tcgaaaccag tgcagagtga
     2101 ggcccgcaac ccgcgacatc gcccccttca gcggaggcgc gtccagcagc cgcccgcccg
     2161 gcatgtagcg gctgccaacg actacctggt atccctggcg gtagtactca accatccggt
     2221 ccacttgtgc gaggtcatcg gaaaggtctg ccatgaccac cagcaccgga ccgctttcga
     2281 cctggttgaa gcccgtgcgg atggccccga ctacacctct ccccacattg ttc
//
LOCUS       DASRKK010000322         5022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_903867, whole genome shotgun sequence.
ACCESSION   DASRKK010000322 DASRKK010000000
VERSION     DASRKK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903867"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFC15_06880"
     CDS             complement(<1..462)
                     /locus_tag="VFC15_06880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM09917.1"
                     /translation="MLLWAIVYVPGLFSPPLLDDADSIHAEAARESIVRHDWTTLYID
                     GVRYLEKAPLMYWGMAASYTVFGTTDWAARLPLALGVLALLLATYSLGKRTLGERAGF
                     WAAVVLAVSVGPYIFTRFIIPDLLVGLWLTLGLDFFLRTLDEEPPSIFSCWG"
     gene            727..942
                     /locus_tag="VFC15_06885"
     CDS             727..942
                     /locus_tag="VFC15_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09918.1"
                     /translation="MTIELTDEQKRMIDLAVASGAYHNPDEVIGTALAMLYEDIEDGI
                     ISDARAGEPRFSLDEVEAELRSLGKIK"
     gene            complement(1311..3110)
                     /locus_tag="VFC15_06890"
     CDS             complement(1311..3110)
                     /locus_tag="VFC15_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZM09919.1"
                     /translation="MDQRSFQKLLRRTVLLPVALLLLLAATLVVEILLLNASIRWVDH
                     TDQVIANARQLIRYMVDMESGIRGYFLTGDTFFLDSYIAAKPQVPEQLALLEQLTSDN
                     PDQAQRIGEIRDLDLRWIRYAENLLLHPNPHTLSSQDYAAGKELMDRIHAKQREILDA
                     EDRLHHARYRRAAVLGNIVIGTAVGLSLLTAVLLFTLTRRELFELSSAYDKHLKAEAE
                     KTQQLQESRESYRITLRSLGDAVVATDAAGNVSFLNPVAEQLTGWDYQAARGRPLREV
                     LRIIDERTRTELEDPVALVRRSQPVVNVSSHVALISRSGQEYPLELTGSPILNDRNQL
                     VGVVAVFRDITQRRQTEQTLRSSERLALAGRLSATIAHEIRNPLDTVSNLIYLLRHEE
                     DPSNASSQYLELASDELARIAQITGQLLTFHREAQAPVEVNLADVLQSVLVLFAPQIR
                     KAHVDVEQRFEAVPLVRGFPGELRQVFSNLVGNALDAMPNGGKLILHLRESSLASDVT
                     RLGVRITILDTGAGIPPGVRKNLFAPFFTTKGEKGTGLGLWVSRGLIEKHEGTIHLAS
                     RMRAGKNGTAFSVFLPFEQELGLLNVPGVPPAA"
     gene            complement(3271..>5022)
                     /locus_tag="VFC15_06895"
     CDS             complement(3271..>5022)
                     /locus_tag="VFC15_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM09920.1"
                     /translation="INRVSGLVLPASTKYLVDDIIGKRHFKLLYPLVGVVLLATIIQG
                     LTSFALTQTLSKAAQKLIAELRRRVQEHVGRLSISYYDANKTGTLVARIMSDVEGVRN
                     LVGTGLVDFAGGLLTAVIAFAVLIRISPVMTAIAFGFLGIFGFGLRKAFRTIRPIFRE
                     RAKINAEVTGRLTESLGGVRVVKGYHAEQREHEVFSSGVERLLQNVFRTLTATSVMSL
                     SSSTLMGVIGAIVMFMGTRQILAGKLTLGGFFTYTMFLGYLAAPLFQVVGIGTQITEA
                     LAGLERTREVLDEIPENEDPNRTVTLPEIRGDVAFQNVTFAYEQKKTVLHDISFRARP
                     GTVTALVGSSGSGKSTIIGLIAAFHTPNQGKVLVDSVDLSTVRLESYRTQLGMVLQDS
                     FLFDGSIRENVAFSKPDATEKDLLSACAIARVDEFAEKYELQYDTIIGERGVKLSGGQ
                     RQRVSIARAILANPRILILDEATSSLDSESEALIQEGLSYLMQGRTTFVIAHRLSTIR
                     RADQILVVEEGRIVEHGSHQSLYQFGGRYYELYTKQHGIEQNLFLAPGEGDATEESAA
                     VLPNGVPADPDPTTLLR"
BASE COUNT         1067 a         1440 c         1558 g          957 t
ORIGIN      
        1 tccccagcac gaaaaaatcg agggaggctc ttcgtccaaa gttcgcagga agaaatcaag
       61 accgagcgtg agccacaacc ccaccagcag gtcgggaata ataaaccgag tgaagatgta
      121 aggcccgacc gaaacggcca gcaccactgc cgcccagaaa cccgcgcgtt ctcccagcgt
      181 ccgtttgccc agggagtaag tggccagcag cagcgccagc acgccgagcg cgagcggcaa
      241 ccgggctgcc cagtccgtgg tcccgaaaac cgtgtagctg gccgccatcc cccaatacat
      301 cagcggcgct ttttccaggt agcgcacgcc gtctatgtag agcgtggtcc agtcgtggcg
      361 gacaatcgac tcgcgcgcgg cctcagcgtg gatcgaatcc gcgtcgtcca gcagcggcgg
      421 cgaaaagaga ccagggacgt aaacgatggc ccaaagcagc acgatccaga gccaacggcg
      481 tcgtggccag ggagagtggc tagggacttt gttcgaatcg gaactggcaa ttgctgatcg
      541 gacttgagga gaggacattc ttggttcgac aggaccccat tataagggag ccatgtcagc
      601 caaaaccggg ctagacttgt cctgagcgaa gtcgcaggaa ccgagcaccc caggtttatt
      661 ggaagtacgg ggtggggcgc acggcaaccc cttgattttc gcggagtcgc cagcgagagt
      721 gccgcgatga ccattgaact gacagacgag caaaagcgaa tgatcgatct cgcggtcgct
      781 tccggggcct accataatcc cgatgaagtg atcgggacgg cgctcgccat gctctacgaa
      841 gatattgagg acggcatcat ctcagacgcg cgggccggag agccccgttt cagtctggat
      901 gaagttgagg ccgaactccg ctcgctcggc aagatcaagt gacgttgtac gaaatcacct
      961 tgccgcgtct gcgaaaaggg aatttcgtga cttgacgccg gatgttcatc gaacgcatcc
     1021 ggccaagaaa tacgtaaact cgccaatgac ccgcgacctc cgggatgcaa gaatcttcac
     1081 ggctataaag accgttggag aatccggatt ggcgattaca gggtcgtcta taccatcaac
     1141 gatgccgcga gaactgtcga tgtgacgcgg atcgcgcacc gccgcgacgc gtatgaacga
     1201 tgagtgccgg gggcgctctc gttcccatta acaacgacgg tgcaactgcc ccgccctgga
     1261 acactcccac ccaagctcct gttgctcggg ttgccgagtc ccgccaatca ctacgccgcc
     1321 ggtggcacgc ctggcacgtt gagcaatccc agctcctgct cgaagggcag aaacaccgaa
     1381 aacgcggtcc cgttttttcc ggctcgcatg cggctggcga gatggatcgt tccctcgtgc
     1441 ttttcgatga ggccgcggct cacccaaagg cccaacccgg tacccttttc acctttggtg
     1501 gtgaagaagg gagcgaaaag gtttttgcgg accccgggag ggatgcccgc gccagtatca
     1561 aggatggtga tgcggactcc caggcgcgta acatcggacg ccaggctcga ttcgcgcagg
     1621 tgcagaatca gcttgccccc attgggcata gcgtcgagag cattgccgac caggttggag
     1681 aatacctgcc gcagttcgcc ggggaatcct cgcacgagag gcaccgcttc gaaacgctgc
     1741 tccacgtcga cgtgggcctt gcggatttgc ggcgcaaata ggaccagcac gctttgtaac
     1801 acgtcagcaa gattcacctc aaccggggct tgcgcttcgc ggtggaaggt cagcagctgt
     1861 cccgtgatct gggcgatgcg ggccagttcg tcactggcaa gctccaaata ctgactcgaa
     1921 gcgttgctgg ggtcttcctc atggcgtagc aggtagatga ggttcgaaac ggtgtcgagg
     1981 ggattgcgga tctcgtgggc gatggtggcc gaaaggcgtc cggccagcgc caggcgctcg
     2041 ctcgagcgta aggtctgctc ggtttggcgg cgttgcgtaa tatcgcgaaa gacggccaca
     2101 actccgacga gctggttgcg atcgttcagg atcggcgatc cggtcagctc gagcggatat
     2161 tcctggccgg aacggcttat aagtgcaacg tggctggaaa cgtttaccac cggctgcgag
     2221 cggcgaacca gggcgaccgg atcttcaagt tcggtgcggg ttctttcgtc gatgatgcgc
     2281 aagacttcac gcagtggccg tcccctggcc gcttggtagt cccagccggt aagctgctcg
     2341 gctacggggt tgaggaacga cacattcccc gccgcgtcgg tggcgacgac cgcgtcgccc
     2401 aggctcctaa gggtgatccg atacgactca cggctttctt gtaattgctg cgttttctcc
     2461 gcttcggcct tcagatgctt atcgtaggcg gaggacagct cgaacaactc gcgacgcgtc
     2521 agggtaaaca gcaggacggc agtcagcagc gacaggccaa ccgccgtgcc gatgacaatg
     2581 ttgcccagca ccgcggcacg gcgatagcga gcgtggtgca aacgatcttc cgcgtcaagg
     2641 atttcgcgct gcttggcgtg gattcggtcc atcaattcct tgccggcagc ataatcttgc
     2701 gacgaaagag tgtgcgggtt gggatgcaac agcaggtttt cagcgtaccg aatccagcgg
     2761 agatcaagat cgcggatttc gcctattcgt tgcgcctggt cgggattgtc ggacgtgagt
     2821 tgttccagca acgccagctg ctcgggaacc tggggcttgg ctgcgatata ggaatcgaga
     2881 aagaacgtgt cgccggtcag aaaatagccg cgaatacccg attccatgtc aaccatgtat
     2941 cgaatcaact ggcgggcgtt ggcaatgacc tggtcggtgt ggtccaccca acgaatggaa
     3001 gcgttgagca acagtatctc gaccacgagc gtggccgcga gcagcaacag caatgccacc
     3061 ggtaggagaa ccgtgcggcg cagcagtttc tggaaactgc gttggtccat atcgactcgt
     3121 tagtctgcgg cagttatggc gcgagcgtcc tgatgataca gcatttctgc gggtcgccag
     3181 aggagaggtg cgcgtcagtc gacagtagcg gaagttcctt ggagcggcgg cgttgcctga
     3241 ggtgcgtccg cgctgcgtcg attgtcagtt ctaacgcagc agagtcgtcg gatcgggatc
     3301 cgcgggaact ccgttcggca gcacggcagc ggactcttcg gtcgcgtcac cttctccggg
     3361 cgccaggaag aggttctgtt cgatgccatg ttgcttggtg tagagctcgt agtagcggcc
     3421 accgaactgg taaagcgatt ggtggctacc gtgttcgacg attcgtcctt cctcgaccac
     3481 caggatctgg tccgcccgac gaatggtgga gagccggtgc gcgatgacaa acgtggtgcg
     3541 cccctgcatc agatacgaga gtccttcctg gatcagtgct tccgactcgg agtcgagact
     3601 cgaagtcgcc tcatcgagaa tcagaatgcg cgggttcgcc aggatggcgc gtgcgatgga
     3661 gactcgctgg cgctgcccgc cagagagctt cacgccgcgt tcgccgatga tggtgtcgta
     3721 ctgcagctcg tatttctccg cgaactcgtc cacgcgggca atggcgcaag cgctgaggag
     3781 atccttctcc gttgcatcgg gcttggaaaa ggccacgttc tcgcggatgc ttccgtcgaa
     3841 cagaaacgaa tcctgcaaga ccatgcccag ttgggtgcgg taagactcca gccggaccgt
     3901 ggagagatcg accgaatcca ccagaacttt cccctggttg ggcgtatgga aggcggcaat
     3961 caatccgatg atggtggact tgcccgatcc cgaggacccg accagcgctg tcactgtacc
     4021 gggacgcgca cgaaacgaga tatcgtgcag gaccgttttc ttttgctcgt aggcaaaggt
     4081 gacattctgg aaggccacgt caccgcgtat ctctggaagt gtgacggtcc gattcggatc
     4141 ttcgttttcg gggatctcgt cgagaacttc gcgggtccgt tccaaacccg ccagcgcttc
     4201 cgtgatctgg gtgccgatgc cgacaacctg gaacaatggc gcggcaaggt atccgaggaa
     4261 catggtgtaa gtgaagaagc cgcccagcgt cagcttaccc gcgagaatct ggcgtgttcc
     4321 catgaacata acaatcgctc cgatgacgcc catgagagtg gaagacgaca ggctcatgac
     4381 cgaagtcgcg gtaagggtgc gaaacacatt ttgcagcagg cgctcgacgc ccgagctgaa
     4441 cacctcgtgc tcgcgctgct cggcatgata gcccttgacg acgcgcactc ctccgagcga
     4501 ttccgtcaga cggccggtga cttcggcatt gatctttgcg cgctcgcgga agatggggcg
     4561 gatggtgcga aaggccttgc gcaggccgaa tccaaagata cccagaaagc cgaaggcgat
     4621 agcggtcatt accgggctga tgcgtatcag gacggcgaag gcaatgactg cggtgagcaa
     4681 cccgccggcg aagtccacca gtccggttcc gacgagattg cgcacgccct cgacatcgct
     4741 catgatgcgc gcaaccaacg tgccggtctt gttggcgtcg taataagaaa tagacagtcg
     4801 cccgacgtgc tcctgcacgc gacggcgcaa ctcggcgata agcttctgcg ctgctttgga
     4861 gagcgtttgg gtcagcgcaa acgacgtcag tccctggatg atcgtcgcca gcaacaccac
     4921 tcccaccagc ggatagagca gtttgaaatg ccgcttaccg atgatgtcgt ccaccaggta
     4981 tttggtggaa gctggaagca caagcccaga gacgcggttg at
//
LOCUS       DASRKK010000323         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1294838, whole genome shotgun sequence.
ACCESSION   DASRKK010000323 DASRKK010000000
VERSION     DASRKK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294838"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VFC15_06900"
     CDS             <1..1288
                     /locus_tag="VFC15_06900"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZM09921.1"
                     /translation="GERLQKYVRAQDTVAKVGGDEFVFALINPGHISRAAAAAERIRR
                     AVAGEFEVQGHVLNVTCSIGISLFPYHGKDGETLIKNAAAAMHCAKENGRNNFRFFTR
                     KLNARAVERLALQNSLRVALEKTEFFLVYQPQVEIATGRITGAEALLRWRHPELGLVA
                     PGKFIPVAEDSGLIVPIGEWVLKTACAQARHWQELGLPALPIAVNVSAVQLRQERFLP
                     VIRRVLDETGLAPQYLELEITEGVLLSNAGKTLSMLQQLANMGLPLSIDDFGTGYSSL
                     SYLKDLPVCKLKIDRSFIRAMTVNPRDAAITAAVISMGNSLNLKVIAEGVENEEQMSF
                     LRAHHCDEIQGYYFSKPLEADDFAKKLRSTSFLQWDSACSSSKLFTATESGPWPTTVP
                     GATRYSPFGKLAILTPHAASTNAAAGSKRRHRRVTP"
     gene            complement(1593..>3642)
                     /locus_tag="VFC15_06905"
     CDS             complement(1593..>3642)
                     /locus_tag="VFC15_06905"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZM09922.1"
                     /translation="YVSPDVVGYEDLDNNGSWRSDPTYGNVWMPTSVEAGWAPYHNGH
                     WVWISPWGWTWVDDAPWGYAPFHYGRWVSLQGNWGWVPGPISYQPVYAPALVAFIGGP
                     GFGVSISLGGGGDVGWFPLGPREAYVPGYNTSPGYFNRVNTSNTNISNTTITNIYNNQ
                     VTNKTTNITNVTYVNRTVPGAVTAVPQSAFRNSEPVARAAVAVNAKQMAAAPVVSRAA
                     VVPTRDSVIGRSAATANKVAQPSAAVANRPVVAKTTPPPPPVSFAKQQQELAKHPGQP
                     LAPHEVQSLRPPNVAAAHPLVKQAPPGKPTKPGNAPVAINPGQPAHAQPANTPPNKPV
                     NAPPPNKSANAPANQGQPAHTDRPPSAQSNNQPSPGNKPGTMNPSHPANAQPTPAVNK
                     PANVPPAQSVAPAPAKQAQPARSDRPPMAQPNNQPGSGNKPGTMNPSHPANAQPTPAA
                     NKPANVPPAQSVAPAPAKQAQPARSDRPPMAQPNSQAPPASQQGRTNANRPANAQPAS
                     EPANKPANVPPARPVASSPTSPVPAPENRPVAPANRSTPPPPASPSVSAPHPPAAPAQ
                     QPGRPPAPANRSTPPPPASPSISAPHPPAAPAQQPGRPVAPANRSTPPPPASPSVSAP
                     HPPVAPAQRPDRPPAPQPSQPPKAQPAPPTAAQSNAQKQQAEKAKAEQQKKEQKSPPQ
                     "
BASE COUNT          639 a         1019 c         1211 g          773 t
ORIGIN      
        1 cggagaacgt ttgcagaagt atgtccgcgc acaggacacc gtggcgaagg tgggaggaga
       61 cgagtttgta ttcgcgttga ttaaccccgg tcatatctcc cgcgcggcgg ccgccgcgga
      121 gcggatcagg cgcgcggtgg ccggtgagtt cgaggtccag ggccatgtgc tcaacgttac
      181 ctgcagcatc ggtatcagcc tgttccctta ccatggcaag gacggcgaaa cgctgatcaa
      241 gaatgcagct gcggccatgc actgcgccaa ggaaaacggg cggaacaatt ttcgattttt
      301 cacccggaaa ctgaacgcca gggctgtgga acgcctggct ttgcagaaca gcttgcgagt
      361 tgcactagaa aagacagaat tctttctcgt ttaccaaccg caagtagaga tcgctaccgg
      421 aagaatcacc ggcgcggaag cgcttctccg ttggcgccat ccggagttgg gtctcgtcgc
      481 ccccggcaag ttcataccag ttgcggaaga tagcggcctg atcgttccca ttggggaatg
      541 ggtgctcaag accgcctgcg ctcaggctcg ccactggcaa gaactgggcc ttcccgctct
      601 gcctatagca gtcaacgtgt cggcggtcca gcttcgccag gaacgcttcc tcccggttat
      661 ccgacgggta ctggacgaaa ctggtctcgc gcctcaatac ctcgaactgg aaatcacaga
      721 aggtgtgctg ctctcgaatg cggggaaaac gctttcgatg ctacagcaat tggccaacat
      781 ggggttgccg ttgtcaatcg acgacttcgg tacaggatac tccagcctta gttatttaaa
      841 agaccttccc gtctgcaagt tgaaaatcga tcgttcgttt atacgagcaa tgacggtcaa
      901 cccacgcgac gccgcgatta ccgccgcggt gatcagtatg ggaaacagcc tcaacctgaa
      961 ggtgatcgct gaaggcgtcg agaacgagga acaaatgtct ttcctgcgag cacaccattg
     1021 cgacgaaatt caggggtact actttagcaa gcccttagaa gccgacgatt ttgcgaaaaa
     1081 gctgcgaagc acgtcattct tgcagtggga ttcagcctgc tcttcgtcga agctttttac
     1141 ggccaccgaa tcagggcctt ggcccacgac ggttcccgga gccactcgct actcgccgtt
     1201 cgggaagctg gcgatactta ccccccacgc cgcctcgact aacgctgctg cgggcagcaa
     1261 aaggcgacac cggcgcgtca ctccatagtt agtattgacc caacggatgg tgctgttgaa
     1321 cgccatccgc ggggtctcgc tcaaagcaag aacgctgaac aggagtccat tggccaaccg
     1381 gacgaaaagg cagaaatctg caccaggagt catgcaggtt gcaacctgca gaggttcttg
     1441 cggcggacaa tggctcgagg caatatttcg tcgctgaccc tgctggggat gctggacgag
     1501 atggcatctt ggtgccgcag gaatcggccg gacgtctttt caggacgccc ggccgattca
     1561 atgttcgatg cagcagatca aaaccgcttc cgctactgtg gtggcgactt ctgttccttt
     1621 ttctgctgtt cggccttggc tttctcagcc tgctgcttct gtgcattcga ttgcgccgcc
     1681 gttggcggtg ccggttgcgc cttcggtggt tgcgaaggtt gaggcgccgg cggacggtca
     1741 ggccgctgcg cgggagctac gggaggatgc ggagcgctga cgctcgggct ggccggaggc
     1801 ggtggggtgc ttctgttagc aggcgccacc ggacggccag gctgctgcgc gggagcggcg
     1861 ggaggatgcg gagcgctgat gctcgggctg gccggaggcg gtggggtgct tctgttagca
     1921 ggcgccggcg gacggccagg ctgctgtgcg ggagcggcgg gaggatgcgg agcgctgacg
     1981 ctcgggctgg ccggaggcgg tggggtgctt ctgttagcag gcgccaccgg acgattttca
     2041 ggtgcgggca ccggacttgt tggcgaactg gcgacagggc gcgccggcgg tacgttggcg
     2101 ggcttgtttg caggctcgct tgccggctgt gcattggccg ggcggttagc gttcgtcctc
     2161 ccctgctggc tggcaggcgg cgcttgactg ttcggctgtg ccataggtgg ccgatcgctg
     2221 cgagcaggct gagcctgctt ggccggtgcg ggcgccaccg actgtgcggg cggtacgttg
     2281 gccggcttgt tggcggccgg ggtcggttga gcgttggccg gatggctggg gttcattgtg
     2341 cccggcttgt tgcccgaccc aggttgattg ttcggctgtg ccataggtgg ccgatcgctg
     2401 cgagcaggct gagcctgctt ggctggtgcg ggcgccaccg actgtgcggg cggtacgttg
     2461 gccggcttat taacggccgg ggtcggttga gcgttggccg gatggctggg gttcattgtg
     2521 cccggcttgt tgcccggcga aggttgatta tttgattgcg ccgaaggcgg ccggtcggtg
     2581 tgcgcaggtt gaccctggtt tgcaggtgca ttcgcgctct tgttaggagg cggcgcgttc
     2641 acaggcttgt tcggcggcgt gttcgccggc tgggcgtgcg ccggctgtcc cgggttaata
     2701 gcgactggcg cgtttcccgg ctttgttggt ttacctggcg gcgcctgctt gacgagcggg
     2761 tgcgctgccg caacgtttgg cgggcgcaag ctctgtactt cgtgtggagc gagcggctgg
     2821 ccggggtgct ttgccagctc ttgctgttgc ttggcaaaag acacgggcgg cggaggcggc
     2881 gttgtctttg ccaccacggg acggttggcc actgctgccg atggttgggc caccttattg
     2941 gcggttgctg ccgaccttcc gataacactg tctctggttg gaaccaccgc agctcgggac
     3001 acgacaggcg cagcggccat ctgttttgcg ttcacggcca cggccgctct cgccaccggc
     3061 tctgagttcc taaaagcact ctgcgggacg gcggtcactg cgccgggtac agtcctattc
     3121 acgtaggtga cgttcgtgat gttcgtagtc ttgttggtaa cttgattgtt gtagatattg
     3181 gtgatggtcg tattgctgat gttggtattg ctggtgttga cgcgattgaa gtacccgggg
     3241 ctggtgttgt acccaggcac ataagcttcc cggggaccga gcgggaacca gcccacgtcg
     3301 ccgccgccac cgagcgaaat tgacacgccg aaacccggtc cgccaatgaa ggccactagc
     3361 gcaggcgcat acacaggttg gtagctgatc ggtccaggca cccagcccca gttaccctga
     3421 agagagaccc accggccata gtggaacggt gcatagcccc aaggagcatc atcgacccac
     3481 gtccaacccc acggagagat ccagacccaa tgcccattgt gataaggggc ccatccggct
     3541 tcaactgaag tgggcatcca aacgttgccg taggttggat ccgagcgcca cgagccattg
     3601 ttatccaagt cttcatagcc gaccacgtcg ggtgagacat ac
//
LOCUS       DASRKK010000324         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_317701, whole genome shotgun sequence.
ACCESSION   DASRKK010000324 DASRKK010000000
VERSION     DASRKK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317701"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..446
                     /locus_tag="VFC15_06910"
     CDS             270..446
                     /locus_tag="VFC15_06910"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZM09923.1"
                     /translation="MTSIRLDTDLADEAAKILGVKSRTEAVHVALREIVATKRFKDLM
                     RKHRGKLSFAGHDE"
     gene            439..852
                     /locus_tag="VFC15_06915"
     CDS             439..852
                     /locus_tag="VFC15_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM09924.1"
                     /translation="MSDLILFDTDIFLDDLRTGRHRQRIESVSGLVRTSSVVLAELWR
                     GARKPAEREFLRALQRNHPVLTPTAGNWLESGQLLAKISAEKGFTPDKLRDMHFDVLI
                     ALTARSNGARLITSNRADFEMIYSYCKFKLEIWAA"
     gene            1009..1785
                     /locus_tag="VFC15_06920"
     CDS             1009..1785
                     /locus_tag="VFC15_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09925.1"
                     /translation="MGKKWLIDAGRVLCAAGGAILIIRTALGSHPAFSKWLLTVAIVL
                     CTLGLISFAVSAVAGLGKPLREGFERVAFRSVESPHSRAFAKIGDLPALHIEYTKHFH
                     DDVPSIDLMRSWIERCNTSFVLVYRETQEPGLSKHQELVGSFKFLPLTAEGIRAMQGG
                     QATGSTFKPEHICAKRGRASAYYIGDVFAGDKTARAVVLHELKGDCLEVSSRSVSFYA
                     RPLTKDGRHVMIRRGFFQVSDGRSKPELGKLCRLDLPATG"
     gene            complement(1883..>2480)
                     /locus_tag="VFC15_06925"
     CDS             complement(1883..>2480)
                     /locus_tag="VFC15_06925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZM09926.1"
                     /translation="LEQDWGYSLFLQFYFFCVIGAALVTVAVSFTGLLGVSPRTLTVG
                     ASGGIYGLLLAFGILHGESQVMLFPLPFLIKAKYFVIGIIALAMYGALSSAQGGGQSI
                     AYMAHLGGALFGYIWLKVVPRRGFGYATSERAFGLRNSYYRWKRKRAARNFEVYMRKH
                     DRSDYRHDDYFDEHGNLRDPSTRDVRKKNGDSTPPWVN"
BASE COUNT          583 a          654 c          714 g          529 t
ORIGIN      
        1 tgcgctcgtc gctcaggatg acacccaaaa aagtacgtgc gttcatgaat aatccaggct
       61 aagccttctt tatggcaata cacaataacc gaaaatggtt gagttaggaa caggcagatt
      121 cgcgggaaag ctctggccgc gggctcaggt gggttcggtt acctgggatg gggggccggt
      181 cgagtcgggc gacatcggta agatacgata tgtatatacg actacgataa gatgtatata
      241 caagcgaggc taagctcatg actgtaacaa tgacatcgat cagactggac acggacctgg
      301 ccgacgaggc agcgaagatc ctgggggtga aatcccgcac cgaggcggtc catgtggcgc
      361 tccgggaaat tgtcgcgacg aagcgattca aggacttgat gagaaagcat aggggaaagc
      421 tgtcgttcgc ggggcacgat gagtgacctc atcctcttcg ataccgacat atttcttgac
      481 gacctgcgga cgggacgcca ccggcagcga atcgaatcgg tcagcgggct ggttagaacg
      541 tcttcggtag tgttggcgga gttgtggcgc ggcgccagaa agccggcgga gcgcgaattt
      601 ctgcgcgccc tgcaacgcaa tcatcccgtt ctcactccaa cggcggggaa ttggctggag
      661 tcgggacaac tgctggcgaa gatcagcgca gaaaaaggat ttaccccaga caaacttcgc
      721 gacatgcatt tcgatgtgct gatcgccctc accgcccgct ctaacggcgc tcgcctgatt
      781 acctccaatc gcgccgattt cgagatgatc tatagctatt gcaagttcaa gcttgaaata
      841 tgggccgctt aggcggacga actgaggttg aggaagactt cttagaacag gttccgcaat
      901 cacacccacg ccgtctacaa ggagaagcat gataaatttc ggaacagagc cgtctcttgt
      961 cgtaaaattt atgttcctta cgctacgtga aatcgtggat gatccgttat gggcaagaaa
     1021 tggcttattg atgccggaag ggtcttatgc gccgcgggag gcgctatcct aataatccga
     1081 accgcgttgg gctcgcatcc ggccttttca aaatggttgt taaccgttgc cattgtgttg
     1141 tgcacccttg gattaatctc gtttgccgtt tctgcagttg ctggacttgg aaagccttta
     1201 agagagggct ttgagagggt cgcattccgc agcgtggaat cgccgcattc tcgcgcgttc
     1261 gccaagatcg gggaccttcc ggcactgcac atcgaataca ccaagcactt tcatgatgat
     1321 gtcccttcta tcgaccttat gcggtcgtgg attgaacggt gcaacacttc attcgtcctg
     1381 gtctatcggg aaacgcagga acccggttta tcgaagcacc aagaacttgt tggttcattt
     1441 aaatttcttc cgctcaccgc cgaaggcata agggcgatgc aaggcggcca ggccaccggt
     1501 tcgaccttca agccggagca tatctgtgcc aaacgtggca gggcgtccgc ctattacata
     1561 ggtgacgttt ttgccggcga caaaacagcc cgggcggttg tgttacatga gctaaagggg
     1621 gactgtcttg aggtctccag ccgaagcgtt tctttttatg cgcgtccact gacgaaagat
     1681 gggcgtcacg ttatgattag gcggggattt ttccaggtgt cggatggcag atcgaagcca
     1741 gaactgggaa aactttgtcg attggacctc cccgcaacgg ggtaagcaca cccttaaaat
     1801 cgattcgaac caagaaaacc ttccacaaag gctggccacc tttaccttat cggcgtctca
     1861 cgagtagcaa tagacgcaat tctcagttta cccacggcgg cgtggagtcg ccgttcttct
     1921 tgcgcacgtc gcgggtgctg ggatcgcgga ggttgccgtg ttcgtcgaag tagtcatcgt
     1981 gccggtagtc gctgcgatcg tgcttgcgca tatagacctc aaagttgcgc gccgcgcgtt
     2041 tccgcttcca acggtaatag gagttccgta gtccgaaggc gcgctccgag gtggcgtagc
     2101 caaaaccccg gcggggcacc accttcagcc agatatatcc gaacagcgcg cctcccaggt
     2161 gcgccatgta ggcgatcgac tgcccaccac cctgtgcgga cgacaacgcg ccatacatcg
     2221 cgagggcgat gatgccgatg acgaagtact tggccttgat gaggaaaggc aacgggaaga
     2281 gcatcacttg cgactcgccg tgcagaatgc cgaaggccag cagcaatccg tagattcccc
     2341 ccgacgctcc cacggtgaga gtccgcggag agacaccgag gaggccagtg aaggaaacgg
     2401 cgaccgtaac cagcgcggcg ccaatcacgc agaaaaaata gaactggagg aacaggctgt
     2461 agccccaatc ctgttccagt
//
LOCUS       DASRKK010000325         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1294935, whole genome shotgun sequence.
ACCESSION   DASRKK010000325 DASRKK010000000
VERSION     DASRKK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294935"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1368)
                     /locus_tag="VFC15_06930"
     CDS             complement(277..1368)
                     /locus_tag="VFC15_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM09927.1"
                     /translation="MRSQDIQEVALYSYISPEQRVPADHPLRPIREMVNVVLRKISRE
                     FDKIYSLGGRPSIPPERLLRALLLQVLYTVRSERMVMEQLDYNVLYRWFVGLNMDDAI
                     WDVTVFTKNRERFLNGDIAEKFFGAVLEQARAAGYLSEDHFTVDGTLIEAWASHKSFR
                     PKDAQPLPIGGCIKAKNPEVDFRGRERSNDTHASTTDPDARLYKKSAGSASKLGYLGH
                     ILTENRNGLVVDTALTLATGTAEREAALQMLGNLPDGSGITLGADKAYDTADFVEQAR
                     AMNVTPHVAQNDKNRRSAIDGRTTRHAGYAISQRKRKRVEETFGWGKVVGLIRKARVR
                     GLARVGGMFTFAMAAYNLVRIRNLQNVAG"
     gene            1538..2848
                     /locus_tag="VFC15_06935"
     CDS             1538..2848
                     /locus_tag="VFC15_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043743199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM09928.1"
                     /translation="MNDELAATDADIARLDGYIDRLSGALGHADRIFPFRSYLIGTML
                     PGKRKSIEPMSARLDPQNVPRMHQKLQHFIATSPWHDDAVLARVREEVIPRMQEREAI
                     QAWIVDDSGMPKKGKLSVGVAHQYCGQLGKQANCQVAVSLSIANSFASLPVAYQLYMP
                     QAWFDDEHAEKREKCKVPVDIAFHTKPQIALQQISEALEADVPRGVVLADAAYGNDTA
                     FRTGLTALAAQYSLNIQETTTAWPDGKAPLKPPRRKSKRGRKPIRIRRTLRRHPVRVR
                     DIAHALPESAFKRVSWREGTAKPLTSRFAATRVRPASRDTALSAPREYEWLLIEWPKD
                     ENAPTKYWLSTMSEETSLVDLVTTSMLRWRIERDYQNLKQELGLGQYEGRSWQGFHHH
                     ATVCIAAYGFLLITQGGFSPWCSPRIRFPQPPVPEDFRPRGAPR"
BASE COUNT          699 a          883 c          825 g          672 t
ORIGIN      
        1 cgtaatcggc tatcgtacca cgttcgtgca cgattcgcga agacgaggag tcgcatgcat
       61 attcgcgccg ataacgtcac gaacgcggga ttatcgtcgg ggcgacagat taaactgctg
      121 gcggttctaa caggttgcgg aaaaagggcg cttttgtagc aaagcctctc ctcttccaaa
      181 tgtttacttt tcgcggtcgc gcttgttttt ctcgattcct gcgagtttcg gggttatttt
      241 gttacttttg gacgcagcta tcccgtaatt tactaattag cctgctacgt tctgcaggtt
      301 ccgtatccga accagattgt aagcggccat cgcgaaggtg aacatgccgc ccacgcgtgc
      361 caatccgcgc acacgcgctt tccgaattag gccgacgacc tttccccagc cgaaggtctc
      421 ctcaacgcgt ttgcgcttgc gttggctgat ggcatatcct gcgtggcgcg ttgttcgacc
      481 atcgatcgcg cttcggcgat tcttgtcgtt ttgcgctacg tgtggtgtaa cgttcatcgc
      541 gcgggcctgc tcaacgaagt ccgctgtgtc gtaggctttg tctgcgccaa gcgtaatgcc
      601 tgaaccatca ggcagattac caagcatctg aagtgccgct tcgcgctcgg ccgtccccgt
      661 cgcaagcgtc agcgcggtat caacgaccag accgttgcga ttctcagtca ggatatgccc
      721 caaataaccc agctttgacg cgctaccggc gctcttctta tatagccgcg catctggatc
      781 ggtcgtcgag gcatgggtgt cgttgctgcg ctcgcgacca cgaaaatcca cctcaggatt
      841 cttggccttg atacaacccc cgatcggcaa cggctgcgcg tctttcggcc tgaagctctt
      901 gtggctcgcc caggcttcga tcaatgtgcc gtcgaccgta aaatggtctt cgctcaagta
      961 gcccgccgcg cgcgcctgct cgaggactgc gccaaagaac ttctcggcga tgtcgccgtt
     1021 gaggaagcgc tcgcggttct tggtaaacac cgttacgtcc catatggcgt cgtccatgtt
     1081 gagaccaacg aaccagcggt agagcacatt gtaatcgagt tgctccatca ccatccgctc
     1141 actgcgaacc gtatagagta cctgcagcag cagcgctcgc aggaggcgct ccggcggaat
     1201 cgatggccgg cctccaagag agtagatctt atcgaactcg cgcgagatct tgcggagcac
     1261 gacattgacc atctcgcgga ttggccgcag cggatggtcc gccggcacgc gctgttcggg
     1321 cgaaatgtag ctatagagcg ctacttcctg aatatcttgg gaccgcatcg gccgctacct
     1381 caacagaatc gaggagactt gccaccatta tactcaccaa tcgtcccggc gcaaagcaaa
     1441 actcggggcg tttttccgca acctgttagt accgctgtgt cataaataat tgaaaattcg
     1501 aaggacttcg cgatgaggaa gttcgaatct cccgtagatg aacgacgaac ttgcagcgac
     1561 tgatgctgat attgcacgac ttgacgggta tatcgatcga ctgagcggcg ctctggggca
     1621 tgccgatcgc atattccctt ttcgctcata tttgatcggc acgatgctac ccggcaagcg
     1681 caagagtatc gaacccatga gcgcgcgact ggacccacag aatgtaccaa gaatgcacca
     1741 gaaactgcaa cacttcattg caacatcgcc ctggcacgat gacgcggtct tggcacgcgt
     1801 gcgtgaggaa gtaattccgc gaatgcaaga gcgagaagcg attcaggctt ggattgtcga
     1861 tgacagcggc atgccaaaga agggcaaact ctccgtcggg gtcgcgcatc agtattgcgg
     1921 acaattggga aaacaagcaa attgtcaggt cgcggtgagc ttatcgattg caaattcatt
     1981 cgcgagcttg cccgtagcgt accagctcta tatgccgcaa gcgtggtttg acgatgaaca
     2041 cgccgaaaaa cgcgagaagt gcaaggtgcc cgttgacatt gcgttccaca cgaagcccca
     2101 gattgcgtta cagcagatca gcgaagcact agaagcagac gttcctcgcg gcgtcgtgct
     2161 tgccgatgcc gcatatggaa atgataccgc tttccggacc ggactgaccg cgctagctgc
     2221 tcaatactca ctgaatattc aggaaacaac cacggcctgg cccgatggta aagcaccttt
     2281 gaaaccgccc aggcgaaaaa gcaagcgcgg aagaaagccg atccggattc ggcgcactct
     2341 ccgccggcat ccggtccgcg ttagggatat tgcgcacgca ttacctgaat cagcgttcaa
     2401 aagagtttct tggcgtgaag gaacggcaaa accattaaca tctcgcttcg cggcgacgag
     2461 agtccgtccg gccagtcgag acactgcgct gtctgcgccg cgcgagtacg agtggcttct
     2521 catcgagtgg cccaaagatg aaaacgcgcc aacaaaatac tggctctcca ccatgagcga
     2581 agaaacgtcc ctcgtagatc tggtcacaac ctcgatgttg cgatggcgca ttgaacgcga
     2641 ctatcagaat ctcaaacaag agttaggatt ggggcagtac gaaggacgaa gttggcaagg
     2701 ttttcaccat cacgcaacgg tctgcatcgc agcatacggc ttcctcctga tcactcaagg
     2761 aggtttttcc ccctggtgca gtcctcgtat ccgattcccg caacctcccg tacccgaaga
     2821 tttccgacca cgcggtgctc cccgttagat ttgtacgaca tgtcgcagac tcaatcgcaa
     2881 caatacgagc tcgcgtcagc aatgcgctgc atgcaatctt accattctgc cacgcatgcc
     2941 agcagcgccc ctatgttcgc aattccgcgc cactattatg ttgagtagtt atgacacagc
     3001 aggtgcgcca ggaaaagctg acagttggct aacgggtgca aatcccggac gacaagggcc
     3061 gtagcgggcc ggtcggtat
//
LOCUS       DASRKK010000326        12916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_806370, whole genome shotgun sequence.
ACCESSION   DASRKK010000326 DASRKK010000000
VERSION     DASRKK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806370"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2636)
                     /locus_tag="VFC15_06940"
     CDS             complement(<1..2636)
                     /locus_tag="VFC15_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZM09929.1"
                     /translation="MRTSRYFAARNGRFIAVVTALLLAGVVSAGAQIVPTNDPPVYGP
                     YNVTSAQGGDGVHKKMVEKDTVLRADSPWTMYAWVQVAAPVTGPQLVAGYGDVAAEYS
                     RYLGLDAGKLMLWMGLDNSFSAPATVTPGKWQFVAATFDGQSVFRLYSDGAQVGQGVL
                     ALGRVAAVVQIAPPVPTPPMAAIPAAPALPEFHGQHFGGNVAGFTIVRAALTAEQIKK
                     LAGSPPNFGVIVFEEGAKPWPVQTRGQAGYRAPQDPATYPKSRAPYSAAVAKPVAAGP
                     ALLERGANRWELADGWTMAAAPTVKATAAEMAAAGFDAKGWMAATVPGTALTTMIDRG
                     VYPDSDYGLNNLLIPESLNKQDYWYRNEFTVPKTSARMTLTFEGINYAAEVWLNEKPL
                     GTIKGAFIRGVFDVTDTLKPGQTNVLLVKVSPPPHPGIPHEQSVLGGPGENGGAMVLD
                     GPTFMATEGWDWIPAIRDRDTGIWQPVVLTASGVVKIGDVQVVTKLPLPDTSKADVEI
                     NVPLENVSDKPVKGTVRVAIESVTVTKDVTVAPGKSEVKLAPAEFAQLTLQTPRLWWP
                     NGYGKPDLYAAKVEFDEDGAASDEKQVRFGVREVTYEVGLLDSNGQLRRVEVSPTAAR
                     ARGEQVVNLTHEGIRQIPTPDPFPEFLPKEWKDYWLAWAASLTPAGETSPAVIPANDS
                     AMEHYLVLKVNGVRIAARGGSWGMDDSRKRVSREKLEPYFRLHREANVNIIRNWMGQN
                     TEETFYELADEYGLMVWNDFWATTQDYNTEPLDIPLFLDNTRDTVLRFRNHPSIVMWC
                     GRNEGVPSPILNKGIIEVLDKYDGTRYYSPSSNQVNLQNSGPYKFQDPRLYFTVWNHG
                     FSVETGTVSLSTMESLEAWMPK"
     gene            complement(3348..4400)
                     /locus_tag="VFC15_06945"
     CDS             complement(3348..4400)
                     /locus_tag="VFC15_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZM09930.1"
                     /translation="MNIRAVIIGCLLMVAPLLARDSTDVIVMKNGDHLTGEIKGMSQG
                     VLYMSMNYILGTSDVQWSKVDHIESKQLFLVKTEDGLVYTGTLATAESAGGRPMTIEV
                     VESSSKQTPLERPTVVEMDQTSEHFWQRFNGEINTDITYTKGNQSTQYNLSSDVGYPR
                     ERWLATASFNSTLSSSTGNTATTRNQVNVGAQRLLRWNNWFYAGEGGLLQSSEQGINH
                     QGNVGGGIGRYLLNSNRTKIYLLGGFAWQQTTYAPSVFVSGAQNVATGLLSGGLKFYR
                     FNKTTIEFTGSVFPAISQPGRVYTNVNASYYIKLSGSLSWNVSFYGNWDNQPPPTFSG
                     SDYGTSSGLSITFGNR"
     gene            4590..5327
                     /locus_tag="VFC15_06950"
     CDS             4590..5327
                     /locus_tag="VFC15_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HZM09931.1"
                     /translation="MSRDGAWGVTSGTPFVRVWLPLTLLVLALSPSAAAQTDQFLPEV
                     DAYYKLTSPVRIWLQAKETSEAGTPVTAEFGPSLDFYIKSPLRLADVTSFDLDDSKSR
                     ALVFSVGYRYLPTPGSPPTNRLEPLFTLNYPVPRIGLLLSDRNRADLDWKIGTFTWRY
                     RNRVQLERTVKVRSYHLSPYASAEFYYESLYGKWSDTAIYAGCLFPIGKHFELNPYYE
                     HQNNTGPSPNQQLNQAGLMLNMFFSRR"
     gene            complement(5373..7004)
                     /locus_tag="VFC15_06955"
     CDS             complement(5373..7004)
                     /locus_tag="VFC15_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09932.1"
                     /translation="MAPKSLLAAKLDLALMKRRVAEGFALLDHSESELAHLQPLHQDA
                     ASVVVRVAQWVDLGYRDVDFLRELVQKFPPDVRGRMALRAYLQLRMAEAFCAMGEEDL
                     DRAIELLHFVLQAEDELGDQRLAAIAHFWKGRSHRKKGEYDEAMAHVVKGRSLMQAIP
                     APKLAAVIQIQESWLLFQKGQSAEALQLLEEARAQLAETDDALSLGNIESAKGRMERQ
                     AGKYADALKHFANAVELYARRDPNHRNLARALVNAASVKRLVALQLRKRIDAQAESSQ
                     SARPGTSSRTRSASGALARHTQICREALEELQRAGEIYSLHRYYGGIASVLVSTGYLH
                     LDSGNIELGAQEALKAYEMAHDKNDHIHMARARTLQCVTENARVDDQLGEDADAAVHA
                     NHARLYADEAVHWAELTQNRHLVAEAYVARGMMFANDFFQEWEEAKRCVDHAAALLGP
                     EDRDHLREDLVVLKTRILRASSIDETLRAWSEGILGEKTFQQVTKEFAEIVIPKVWQR
                     EGKKVSKVSSRLSVSPKKVRRILRNVGLLDLGENG"
     gene            7244..8623
                     /gene="aceA"
                     /locus_tag="VFC15_06960"
     CDS             7244..8623
                     /gene="aceA"
                     /locus_tag="VFC15_06960"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZM09933.1"
                     /translation="MNQPPNPNEHDWNSPRWKGITRSYTPADVDRLRGTVHIEHTLAR
                     LGAEKFWKLLRTEPYIPALGALTGNQAVEMAQAGLKAIYLGGWQVAADGNLGGNMYPD
                     QSLYPANSVPAVVRSINNALLRADQISHSEGRPEFDWMLPIVADAEAGFGGNLNAFEL
                     TKAMIESGAAAVHFEDQLSSAKKCGHLGGKVLVPTEEATQKLVAARLAADTMGVPTVI
                     IARTDANSAGLITSDYDARDRNFITGERTVDGFYIYRGGLQAAIARGLAYAPYADLLW
                     CETSEPNLNEAREFANAIHAQFPGKMLAYNCSPSFHWRAKLNEEDIACFQRDLAAMGY
                     RFQFVTLAGFHALNLSMYQLARGYVRSGMTAYSQLQQREIEMAESVGYPAVKHQRFVG
                     TGYFDAVATTISNGQTSTCALPGSTEEDQFEMHTEDYNDGEATERTPMPNLYMLLESM
                     PLPSTAAND"
     gene            8699..10282
                     /gene="aceB"
                     /locus_tag="VFC15_06965"
     CDS             8699..10282
                     /gene="aceB"
                     /locus_tag="VFC15_06965"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZM09934.1"
                     /translation="MTTQTEAIRIAGPHSDAQPGILTPQATEFLLALHTRFEPVRQAL
                     LQERKNRQARLDAGQLPQFLDASLPTREASWKVAPIPPDLLDRRVEITGPPDRKMIIN
                     ALNSGASVFMADFEDSNSPTWTNCIEGQHNLRDAVAGTIEFRSPEGKLYQVGAKPAVL
                     FVRPRGWHLNEKHVLINGTPISGSLFDFGLFFFHNAKTQIASGIGPYFYLPKLESHQE
                     ARLWNDVFVFAQEYVSIPKGTIRATVLIETITAAFEMDEILYELRDHSAGLNCGRWDY
                     IFSFIKKFRNHASHVLPDRGVVTMEQPFLHSYVELLIHTCHRRGIHAMGGMAAQIPIR
                     NNPEANEKAMERVKQDKLREVRAGHDGTWVAHPGLVPVAKTIFDEYMPTANQIAPPAN
                     PRSPITAANLLELPKGAITEAGLRRNIDVGLQYLEAWLRGNGCVPIYDLMEDAATSEI
                     CRSQIWQWIHYGARLDDGRVITAAMFDQMLREVVERLRSAETTPPDHKLDRAAELLTN
                     MSKGEYEEFLTSVAYLDLA"
     gene            10391..10894
                     /locus_tag="VFC15_06970"
     CDS             10391..10894
                     /locus_tag="VFC15_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09935.1"
                     /translation="MSLALTVCAANVYGQTVTTIQRVYIADASGTEILNDPGTVHLVD
                     SHGTDIVPPKITGQKSCDSPRISADKQTAGWQANLENCCTSYDIPLTLVLYRAGNIVQ
                     TIAPGQMIYDWQFWTNDKVAISSGPTHGDFGRHLGLYDVATGKLLKEWMGEQGDTPPA
                     WGRDLRQ"
     gene            11014..11496
                     /locus_tag="VFC15_06975"
     CDS             11014..11496
                     /locus_tag="VFC15_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09936.1"
                     /translation="MKKLEAVEEARVIMTAGTEWGVFKWLMEKRKVRGIADRATAALN
                     EAEDKVKATWSDELKRAYNYLATQDGDATKGRRGAKSKEATKPSDPEILAIAQKVLEA
                     DEEAERQRLDAEDTFAEGERQMSTSMAREGARKALTTYDLHEKAIRKAEAAARAKVSP
                     "
     gene            11673..12146
                     /locus_tag="VFC15_06980"
     CDS             11673..12146
                     /locus_tag="VFC15_06980"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM09937.1"
                     /translation="MLSWLLRDPLPVGSKAPDFTLLTESGKKVKLSALHGKNVVLVFY
                     PGDDTMICRRQLCEFRDAWPAIHGLNVVVFGVNPQSAQSHREFSERHEFPFPLLVDAG
                     QQVAALYNCDGGMVNRTVYLIGPDGLIRYARRGMPQPQEVLAAVAGKKRLATSLR"
     gene            12233..>12916
                     /locus_tag="VFC15_06985"
     CDS             12233..>12916
                     /locus_tag="VFC15_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZM09938.1"
                     /translation="MNRDNDNFDFDFIVIGSGFGGSVTAHRLVEKGYRVAVMEMGRRW
                     TPENLPKTSWSFQRWFWRPGLALRGFFNMRFFRHVTIMHGCAVGGGSITYASTMLRPP
                     DKVWESGSWKGLDDWKSEMPQHYDTASQMLGVTVNKILGPADRLLKTVADSIGVGHTF
                     YRTSVGIFQAPEGVQGGVTVPDPYFGGEGPERTTCQACGGCMMGCRHGAKNTLDMNYL
                     YLAEKRGAQV"
BASE COUNT         2704 a         3983 c         3578 g         2651 t
ORIGIN      
        1 tccttgggca tccacgcctc cagcgactcc atcgtcgaaa gcgacaccgt tcccgtttcc
       61 accgagaacc cgtggttcca aaccgtgaag tacagccgcg ggtcctggaa cttgtacgga
      121 ccgctgttct gtaaattcac ctggttcgag ctgggcgagt aatagcgcgt gccatcgtat
      181 ttgtcgagga cttcaatgat ccccttattc aatatcggag acggcacacc ctcattccgg
      241 ccgcaccaca ttacgatgga cggatgatta cggaaccgca atacggtatc gcgcgtgttg
      301 tcgagaaaca gcgggatgtc gagcggctcg gtattgtagt cttgcgttgt tgcccagaaa
      361 tcgttccaca ccatcagccc gtactcatcg gccagctcgt aaaacgtctc ctccgtgttc
      421 tgccccatcc agttgcggat gatgttgacg ttggcctcgc ggtgcagccg gaagtacggc
      481 tccagcttct cgcgtgacac ccgcttgcgc gaatcgtcca tgccccagct tccgccgcgc
      541 gccgcgatgc gcaccccgtt caccttcagc accaggtagt gctccattgc actatcgttc
      601 gccggaatca ctgcgggcga tgtctcaccc gctggtgtca gcgacgccgc ccacgccagc
      661 cagtagtcct tccactcttt cggcaggaac tccgggaacg ggtccggcgt cggaatctgc
      721 cgtatgccct catgcgtgag gttcacaacc tgctcacccc gcgcccgtgc cgccgtcggc
      781 gaaacctcca cccgccgcaa ttgcccgttg ctgtccagca gacccacttc gtacgtcact
      841 tcgcgcacgc caaagcgcac ttgcttctcg tcgctcgccg caccgtcttc gtcgaactca
      901 accttcgccg cgtacaggtc aggtttgccg tagccattcg gccaccacag tcgcggggtc
      961 tgtagcgtca actgcgcaaa ctccgcggga gccagcttca cctcgctctt ccccggagcg
     1021 accgtgacat ctttcgtgac ggtcacgctc tcaatcgcga cgcgcactgt tcccttcacc
     1081 ggcttgtccg acacattctc cagcggtacg ttaatctcga cgtccgcctt gctggtgtct
     1141 ggaagtggga gcttcgtcac cacctgcaca tcgccgatct ttaccacgcc cgatgccgtc
     1201 agtactaccg gctgccagat gccggtatcg cgatcacgaa tcgccggaat ccaatcccaa
     1261 ccctcggtcg ccatgaacgt cggcccatcc agcaccatcg caccgccgtt ctcgcccggg
     1321 ccgcccagta ccgactgttc atgcgggatc cccggatgcg gcggcggcga taccttcacc
     1381 agcaatacgt tcgtctgtcc cggcttcagc gtgtccgtca cgtcaaacac gccgcgaata
     1441 aacgcaccct tgatcgtccc cagcggcttt tcgttcagcc acacctcggc cgcgtagttg
     1501 atgccctcaa atgtcagcgt catccgcgca ctcgtcttcg gcaccgtaaa ctcgttgcga
     1561 taccaataat cctgcttatt taagctctcg gggatcagca ggttgttcag cccgtagtcc
     1621 gaatccgggt acactccgcg atcgatcatc gtagtcagcg ccgtgcccgg aaccgtcgcc
     1681 gccatccagc ctttcgcgtc gaaacccgcc gcagccatct cggcggccgt cgccttcacc
     1741 gtcggagccg ccgccatcgt ccatccatcc gccagctccc accggttcgc accccgctcg
     1801 agcagcgctg gccccgcggc caccggcttc gccaccgccg ccgaatacgg cgcacggctc
     1861 ttcggatatg tcgccgggtc ttgcggcgcg cgataccccg cctgtccccg cgtttgcacc
     1921 ggccacggct tcgcgccttc ctcgaacaca atcaccccaa aattcggcgg actgcccgcc
     1981 agcttcttga tctgctcggc cgtcagcgcc gcgcgcacaa tggtgaaccc cgccacattc
     2041 cctccgaagt gctgcccatg aaactccggc agtgccggtg cggcaggaat cgccgccatt
     2101 ggtggcgtcg gcaccggcgg cgcaatctgc acgaccgccg caactcttcc cagcgccagc
     2161 accccctgtc ccacctgcgc tccatcactg tacaaacgga acaccgactg tccgtcaaat
     2221 gtcgctgcca caaactgcca cttccctgga gtgacggtcg cgggcgcgct gaagctgttg
     2281 tcgagcccca tccacaacat cagctttccg gcgtcgagtc cgaggtagcg cgagtactcc
     2341 gccgccacat cgccataccc cgccaccaac tgcggtcccg tcaccggcgc cgccacctgc
     2401 acccaggcat acatcgtcca cggcgaatcc gcccgcagca cggtgtcctt ctccaccatc
     2461 tttttatgga caccgtcacc tccctgcgcg ctggtgacgt tatacggccc gtagaccggc
     2521 ggatcgttag tgggtacgat ttgtgcaccc gcggaaacca cacccgcaag caggagagct
     2581 gtgacaactg caatgaagcg gccattacgc gctgcaaaat accgcgaagt acgcatgaat
     2641 cctctttcca gccatgatcg ccgcggaaat cgaatcggac ttcaacaccc attccaggcg
     2701 ccatttttcg aaagcaaatc atactagagc gcgttaggaa atgacgtagg actcggatca
     2761 gagctgccgt caggttcggt cgtcgaacgg ctaaatcgag acggcaatcc gagtattgtt
     2821 tctcaatccg ttcgggggga gcgcgaaagg agttggacta tgacgaaatg aggaaccgtc
     2881 gccaaaattt ttttcacgcc tccttttgtt ttcaacgatt tgggcgtaaa ttgattgcaa
     2941 acgattgatt acaagtgttt tatagctatc catgtctgcc ataaggactt acggctacgc
     3001 tttccggcaa attcacccaa gttgcgtgag gtttcgagat ttcaaagaac ctacgaggcc
     3061 agtgaacggg actgacccga accgttgtcc gctgccggaa tgacagcgtc cgacgcggcg
     3121 agttccgccc ctgaggcaaa acatcccagt ttcatggata gagcaacgta gagtaggagt
     3181 cacgcaaaat ccatacggcc acgaaactaa agtaaccccg aggcgcaggg acagacgacc
     3241 tcacaccgtc gtaccgctgc gtcacggaag cgggatgacg ccagcccccg cggcggacgc
     3301 gcaagctaac cggagctaac tcatcgctga gtccacgtcg cccggggcta tctgtttccg
     3361 aacgttatgc tcaggcccga gctggtgccg tagtcgctgc cggagaacgt cggcgggggc
     3421 tggttgtccc agttgccata aaacgacaca ttccacgaca aactacccga aagcttgatg
     3481 taatacgacg cgttcacgtt ggtgtagacg cgtccgggtt gggagattgc gggaaaaacg
     3541 cttcccgtaa actcgatggt ggtcttgttg aagcgataaa acttcaggcc gcccgacaat
     3601 aatccggttg ccacgttctg ggcgccgctg acgaagacag acggcgcata agtcgtttgc
     3661 tgccacgcga acccgcccag caagtagatt ttcgtgcggt tgctattcag caagtaccgt
     3721 ccaatacccc cgcctacgtt tccctgatgg ttaatgccct gttcggaact ttgcagcagc
     3781 ccgccttctc cggcgtaaaa ccaattgttc cagcgcagca gtcgttgcgc ccccacgttc
     3841 acctgattac gggttgtggc cgtatttcct gtgctgctcg acagtgtcga gttaaacgac
     3901 gcggttgcca gccagcgctc gcgaggatag ccaacatcag agctcaggtt gtattgggtc
     3961 gattgattcc ccttggtata agtgatgtcg gtattgatct cgccattaaa tcgttgccag
     4021 aagtgctctg atgtctggtc catctccacc accgtaggcc gttccagcgg cgtttgcttg
     4081 ctggaagatt ccaccacctc aatcgtcatc ggtcgtcctc ccgcgctctc ggcggtcgcc
     4141 agcgtccccg tatacaccaa gccgtcttcc gtcttcacca ggaagagttg cttgctctcg
     4201 atgtggtcca ccttcgacca ttgcacatcg ctggtgccaa ggatgtagtt catgctcatg
     4261 tacagcacgc cctgcgacat ccctttgatc tcgcccgtca ggtgatcgcc gttcttcatg
     4321 acgataacgt cagtgctgtc ccgcgccagc aacggggcaa ccatgagtaa gcaacctata
     4381 attacggctc ggatgttcat tccggacctg cctgtctcgt aattcggcac tccgccacgc
     4441 gaaataaccc ggcggcgaag gccatcaatt tggtttcgat catctcactg tacccactca
     4501 tttgctaact gtcgaaacca atgattgtta acggacatac aagatccaaa tagctacaca
     4561 aaccgcggaa agtacgattg aatgaactta tgagtcggga tggcgcttgg ggagtgacga
     4621 gtggcacgcc gtttgtgcga gtatggctac cgctgaccct gctggtgctg gcactgagcc
     4681 cttccgcagc cgcgcagaca gatcagttct tgccggaggt cgatgcgtac tacaagctga
     4741 cttcccccgt tcgcatatgg ctgcaggcga aggagacgag cgaagcgggc actccggtca
     4801 cggccgagtt cggccccagc ctcgatttct acatcaagtc gccactgaga ctggcagacg
     4861 tcacctcctt cgatttggac gactcgaagt cccgagcgct ggtattctcc gtcgggtatc
     4921 gttacctgcc caccccgggc agtccgccca caaatcggct ggaaccgttg ttcacgctga
     4981 actacccggt cccgaggatt ggtctcttgc tgtcggatcg taaccgagcg gacttggact
     5041 ggaagattgg caccttcacc tggcgttatc gcaaccgcgt tcaactcgag aggaccgtga
     5101 aggttcgttc gtatcacctg tcaccgtacg ccagcgcgga attctattac gagagcctgt
     5161 atggaaaatg gagcgacacc gcgatctatg ccggctgctt attccccatt ggcaagcact
     5221 tcgaactcaa tccctactac gagcatcaga acaatactgg gccgagccca aaccaacagt
     5281 tgaaccaagc gggactgatg ctcaacatgt tctttagccg gcggtgagca tttccggtgg
     5341 tgaaatctta gcgtcggctg gcgatccgga actcacccgt tttcgcctag atcgagaagt
     5401 cctacgttgc ggagaatgcg ccgcaccttt ttcggtgaga cggacaaccg actcgacacc
     5461 ttcgacactt tcttcccttc ccgctgccac acctttggga tcacgatttc agcgaattct
     5521 ttcgtgactt gctgaaatgt cttttcgccc aggatgccct cagaccaagc tcgcagcgtc
     5581 tcatcgatac ttgaggcccg tagaatgcgg gtcttcagca ccaccaagtc ttccctcaga
     5641 tggtcgcggt cctcagggcc gagcaacgcc gcggcatgat cgacgcatcg cttggcctct
     5701 tcccactcct gaaaaaagtc gttggcaaac atcatgccgc gcgccacgta ggcctccgcc
     5761 accaggtggc ggttctgcgt tagctccgcc caatgcactg cctcgtccgc gtacaagcgc
     5821 gcatggttgg cgtggacggc agcatccgcg tcttctccga gttggtcgtc cacgcgggcg
     5881 ttttcggtca cgcattgcag cgtccgggca cgggccatgt ggatgtgatc gtttttgtca
     5941 tgtgccattt cataggcctt aagcgcctcc tgggctccca gctcgatgtt gccgctatcg
     6001 aggtgcagat atccggtact caccaacacc gaagcgatcc cgccgtaata gcgatgcagc
     6061 gaataaatct ctccggcgcg ttgcagttct tccagcgcct cacgacaaat ttgcgtgtgc
     6121 cgggccaacg cccccgaggc acttcgcgtt cgcgacgaag ttcccggcct tgccgactgc
     6181 gacgattccg cctgggcgtc gatacgcttg cgcagttgca gcgcaaccag tcgcttgacc
     6241 gatgccgcat tgaccagcgc ccgcgccaga ttgcgatgat tgggatcgcg ccgggcatac
     6301 aactcgaccg cattggcaaa atgcttcaac gcgtccgcgt attttcccgc ctggcgttcc
     6361 atgcggccct tggccgactc gatgttgccc agagacagag catcgtcagt ctcagccagt
     6421 tgcgcccttg cctcttccag aagctgcagt gcctcggccg attggccctt ctggaacagc
     6481 agccagctct cctgtatctg gattaccgcc gcgagcttgg gcgccgggat cgcctgcatt
     6541 aagctgcgtc ccttgacgac gtgcgccatg gcctcgtcat actcgccctt cttgcgatgc
     6601 gacctgccct tccagaaatg cgcaatagcg gcgaggcgct gatcgcccag ttcgtcttcg
     6661 gcctgcagca caaagtgcag caattcgatc gcgcggtcga ggtcttcctc ccccatggcg
     6721 caaaaggcct cagccatgcg caactgaaga taggcacgca gagccatgcg gccgcgaaca
     6781 tccggcggaa acttctgcac caattcgcgc agaaaatcga catcgcggta gccaaggtcc
     6841 acccactgcg cgacccgcac cacgacgctc gctgcgtcct gatggagagg ttgcagatga
     6901 gcgagttcgg attcgctgtg atcgagcagg gcaaatccct cggcgacccg acgcttcatg
     6961 agcgcgagat cgagtttcgc cgccaggagt gatttggggg ccatgccgcg attgtaagcc
     7021 gccaatccac ttcggtctag ctgggagtca agcgaccaca agcctggatt gaaacacccc
     7081 tcgcacacga ccgacgtata tgaatgacaa tgaatgacaa tacgcccata tgcaggtcat
     7141 cccatgggtc aactcggcta ctagtccgaa acggacgtgc gcctcttgcg cgcagttgca
     7201 gttcgtcgtc taatcgtcag gcaagcaact ctcggagacg acaatgaatc aaccaccaaa
     7261 cccgaacgag catgactgga attcaccccg ttggaaaggc atcacccgtt cctacacgcc
     7321 cgccgatgtc gatcggctga ggggcacggt ccacatcgaa cacacgctgg ctcgattggg
     7381 ggccgagaaa ttctggaagc tgcttcgcac cgaaccctac atccccgcgt tgggcgctct
     7441 caccggcaac caggccgtgg agatggcgca agccggtctg aaggccatct acctgggcgg
     7501 ctggcaggtc gccgccgatg gcaacctcgg cggaaacatg tatcccgacc agagtttgta
     7561 tccggccaac agcgttcctg ctgtggtgcg gtcgatcaac aacgcgctgc tgcgggcgga
     7621 ccagatttcg cacagcgagg gacgtcccga gttcgattgg atgcttccca tcgtggccga
     7681 cgccgaagct ggttttggcg gcaacctcaa tgcgtttgag ttgaccaagg cgatgatcga
     7741 atccggagcg gcggcagtcc acttcgagga ccagttgtct tcggccaaga agtgcggaca
     7801 cctcggtggc aaggtgctgg ttcccacgga agaagccaca cagaagctgg tggccgcgcg
     7861 tctggccgcc gacaccatgg gtgtgccgac ggtaatcatc gcgcgcaccg acgccaactc
     7921 ggcgggcttg atcaccagcg actacgatgc ccgcgaccgt aacttcatta cgggtgagcg
     7981 cacggtcgat ggcttctaca tttatcgcgg aggtctgcag gcagcgattg cgcgcgggct
     8041 tgcttacgca ccgtacgccg atttgctgtg gtgcgagacg tcggagccga acctcaacga
     8101 ggcacgcgag ttcgccaacg ccattcacgc gcagttcccc ggcaagatgc tggcttacaa
     8161 ctgctctcct tcgtttcatt ggagggcgaa gttgaatgag gaggacattg cctgcttcca
     8221 acgcgacctg gcggccatgg gatatcgctt ccagttcgta acgctggccg gcttccacgc
     8281 gctcaatctg tccatgtacc aattggcacg gggctacgtc cgctccggca tgacggcgta
     8341 ttcgcaactg cagcagcggg aaatcgaaat ggccgagagc gttggttatc cggcggtgaa
     8401 acatcagcgc ttcgtgggca ccggctactt cgatgccgtc gccacaacga tctccaacgg
     8461 acagacgtca acctgcgctc tgcccggttc cacagaagaa gaccagttcg agatgcacac
     8521 cgaggattac aatgacgggg aagcgacaga gcgcactccc atgccgaacc tttacatgtt
     8581 gctcgaatcg atgccgctac ccagcactgc agcaaacgac tgaggcatac atcggacgga
     8641 acggcttggc gacgcgtaat taacactgcc gtcttgttgt tcggtcaaag gacgatgcat
     8701 gactactcag actgaagcga tccgtattgc gggaccccac tcggatgccc aacccggcat
     8761 tctcaccccg caggcaactg agtttctgct ggcattgcac acgcgcttcg aaccggtgcg
     8821 ccaggcactg ttgcaggaac gaaaaaatcg tcaggcgcgc ctagacgccg gccagttgcc
     8881 ccaattcctg gatgcctccc tgccaacccg ggaggcctcc tggaaggtgg ccccgatacc
     8941 cccagacctg ctcgatcgcc gcgtggagat taccggccca cccgatcgga agatgatcat
     9001 caacgcgctc aactcgggtg ccagtgtctt catggccgac ttcgaggact cgaactcgcc
     9061 cacgtggacc aactgcatcg agggacagca taatctccgc gacgcggtgg cgggcactat
     9121 tgaatttcgt tcgcccgaag gcaagcttta tcaggtaggc gcgaagcccg cagtgctgtt
     9181 tgttcggcct cgcggctggc atctcaacga aaagcacgtg ctcattaatg gcactccgat
     9241 ctcaggctcg ctgtttgatt ttggattgtt cttcttccac aacgccaaga cgcagatcgc
     9301 ctctggcatt ggtccttact tttacctgcc gaagttggag agccaccagg aagcccggct
     9361 gtggaacgac gtgttcgtat ttgcgcagga atacgtgagc ataccgaagg gcacgattcg
     9421 cgcaaccgtc ctaatcgaga ccattaccgc ggcctttgag atggacgaga ttctctacga
     9481 actgcgtgac cattcggccg gactcaactg cggacggtgg gattacattt tcagcttcat
     9541 caagaaattc cgtaatcacg ccagccacgt tctccctgat cgcggagttg tcaccatgga
     9601 gcagccgttc ctgcactcct atgtggaact gctgattcac acgtgccatc gccgcggcat
     9661 tcacgccatg ggcggcatgg cggcgcagat ccccatccgc aacaatcctg aggccaacga
     9721 aaaggcgatg gaacgtgtga aacaggacaa gctgcgcgag gtccgtgccg gccacgatgg
     9781 aacctgggtc gcacacccgg gactggttcc ggtggcgaag accatcttcg acgaatacat
     9841 gccgactgcc aaccagattg cgccgccggc caacccgcgt tcacccatca cagctgccaa
     9901 cctgctggag ctgcccaagg gcgcgatcac cgaagcgggc ttgcgccgca acatcgatgt
     9961 ggggctgcag tatctcgagg cgtggctgcg ggggaacgga tgcgtcccga tttatgactt
    10021 gatggaggac gcagcgacgt cggagatttg ccgttcacaa atctggcagt ggatccatta
    10081 cggcgctcgc ttagacgacg ggcgtgtgat cacggctgcg atgttcgacc agatgttgcg
    10141 agaggtggtg gaacgattgc gttccgccga gactacccct cctgaccaca agctcgatcg
    10201 tgcggcggag ttgctcacca atatgtcgaa gggtgaatac gaggaatttt tgaccagcgt
    10261 ggcgtatttg gatctggcgt agggttactg gttgctggtg accggtatga ccagcgacga
    10321 atcaatttca acgcggagag cacgcttaga gccgatgttg cggttcttcg ggcggcttcc
    10381 aatcctactt gtgtcactgg cgctaacggt gtgcgcggcg aacgtctacg gccaaactgt
    10441 cacaacgatc cagcgagtgt acatcgcgga tgcaagcggc actgaaatac tgaatgaccc
    10501 cggcactgtg catctggtgg acagccacgg cacggacatc gttccaccga aaatcacggg
    10561 ccagaaaagc tgcgattcac caagaatttc ggctgacaag caaactgctg gctggcaggc
    10621 gaacttagag aactgctgca cttcatacga catcccgcta acgctcgttc tctatcgggc
    10681 gggaaatatc gtacagacta ttgctccggg ccagatgatt tacgactggc agttctggac
    10741 gaatgacaaa gtcgcaattt ccagcggtcc gacacatggt gatttcggac ggcatctggg
    10801 cctctatgac gtcgccaccg ggaagttgct caaggaatgg atgggggagc agggtgatac
    10861 acctcccgca tgggggagag atttacgcca gtaggcgcac cttcccgaaa tgggaatcat
    10921 ggcgacatcg ttccggcgtc acagagatat tccaacgctg cagcctacaa tgtcacgtta
    10981 gcgtgtgact tcaaatcctg gactgttacg actatgaaga aactggaagc tgtggaagaa
    11041 gctcgggtaa ttatgactgc gggcacggaa tggggcgtgt ttaagtggct catggaaaaa
    11101 cgcaaagtgc gaggaatcgc cgatcgcgcc accgccgccc ttaatgaagc cgaggacaag
    11161 gtcaaagcca cttggagcga cgaactcaag cgggcttaca actacctggc aactcaggac
    11221 ggcgacgcca ccaagggaag acgaggcgcc aagtccaaag aagcgacaaa gccgagcgat
    11281 cctgagatct tggcgattgc ccagaaggtg ctggaggcgg atgaagaggc ggaacgccag
    11341 cgtctcgacg ctgaggatac cttcgcggaa ggcgagcgcc agatgagcac cagcatggcg
    11401 cgcgaaggag ctcgcaaggc cctcacaact tacgatctcc acgaaaaggc tattcgcaag
    11461 gccgaagctg cggcacgtgc aaaagtgagc ccgtaagcag tcggaggcga cgaggtggcg
    11521 aaaaatgaat gtgctaaccc ttgtcgaatg acatggatag ctataactat attttccgtc
    11581 aatcacttcg agacaatttc ttgctaagtg attgattcta aacaacagca tgaaaagtat
    11641 tgttggcaaa tcgtcaagaa atggcctctg aaatgctctc ttggttacta cgtgatccgc
    11701 ttcccgtcgg gtcgaaagcc cccgatttta ctttgctcac cgagtccggt aagaaggtga
    11761 agttgtccgc cctgcatggg aagaacgttg tgctcgtttt ttatcctggt gatgacacta
    11821 tgatctgccg ccgccagttg tgcgaattcc gcgacgcgtg gcctgcgatt cacggtctaa
    11881 atgtcgtcgt cttcggcgtc aatccgcaaa gcgcacagag ccatcgtgaa tttagcgaga
    11941 gacacgagtt tccctttccg ctgctggtgg atgcaggcca gcaggtggct gcgttgtaca
    12001 actgcgatgg cgggatggtg aaccgcacgg tgtacttgat cgggcctgac gggctgatcc
    12061 gttacgcgcg gcgcggcatg ccgcaaccgc aggaagtgtt ggcagcggtg gcgggaaaga
    12121 agcgactagc aacgagcttg cggtgactgg tagccggtaa ctggtaactg gtgattagta
    12181 attagtattt agtagttggc ggcgctgata aggacgcaag tccagacaac ctatgaatcg
    12241 tgacaacgac aattttgatt tcgacttcat cgtcatcggc tccggttttg ggggcagcgt
    12301 caccgcgcat cgtctggtgg aaaagggtta ccgcgtggcc gtcatggaga tgggccgccg
    12361 ctggacgccc gaaaatcttc ccaaaaccag ctggtcattc caacgctggt tctggcggcc
    12421 gggcctggcg ctgcgcggct tcttcaacat gcgctttttc cgtcacgtca ccataatgca
    12481 cggatgcgcc gtcgggggag gatcgatcac ctacgccagc accatgctgc gtcctcctga
    12541 caaagtttgg gaaagcggtt cctggaaagg actggacgac tggaaatcgg aaatgcccca
    12601 gcactatgac accgcatcgc aaatgctggg cgtcaccgtt aataagatcc tggggccggc
    12661 ggaccgcctg ctgaagaccg ttgccgattc cattggagta ggccatacgt tttaccgcac
    12721 gagcgtcggc atcttccagg caccggaggg agtgcagggg ggagtcacgg tccctgaccc
    12781 gtacttcggc ggcgagggac ccgagcgaac cacgtgccaa gcttgcggcg gctgcatgat
    12841 gggttgccgc cacggggcga aaaacaccct cgacatgaat tatctgtatc ttgccgagaa
    12901 acgtggcgcc caggtg
//
LOCUS       DASRKK010000327         6325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_904421, whole genome shotgun sequence.
ACCESSION   DASRKK010000327 DASRKK010000000
VERSION     DASRKK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904421"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..216)
                     /locus_tag="VFC15_06990"
     tRNA            complement(142..216)
                     /locus_tag="VFC15_06990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Asn,seq:gtt)
     gene            complement(534..1418)
                     /locus_tag="VFC15_06995"
     CDS             complement(534..1418)
                     /locus_tag="VFC15_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM09939.1"
                     /translation="MIGISIRRKARQFSTLVGRSAVTPRSGNSRKPVLATREQLGVTF
                     IGHSSFFLQIGGKNILVDPNFARWIFVLKRLRRPGLRMRDLPPIDAVLVTHAHFDHLH
                     RPSLRTIANLTRQKSGKRPIVVVPNNLRDLVGDLGFSRVVELEWWQNFQLGNVEITHT
                     PSRHWGARVVRDTHRGYGGYVLRAGRNSVYHAGDTAYFDGFKEIGERLNPELALLPIG
                     AYHPASFRNVHTSPEDAIQAFQDLGARWMVPMHYGTFRLSYEPVDEPVQRLLAGAKQH
                     GIGTRICVMEEGVTKFFE"
     gene            1539..2414
                     /locus_tag="VFC15_07000"
     CDS             1539..2414
                     /locus_tag="VFC15_07000"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM09940.1"
                     /translation="MRIAFLGLGIMGRPMASNLVKAGHEVSVWNRSARQHIDGARTAI
                     SPADAAQDAEIVWMCVADTAAVERLVFSEDGIEQSLREGMVIADSSTISPSATLRFAQ
                     RVAEKGVQWIDAPVTGSKIGAENGQLIFIVGGPAETVQAMEPLFKAMGKTVIHVGDTG
                     KGQAAKICMNLQIAMIYEGFAEGMTLARKLGVAPEKMVELVQASMIRSGVVDYKAPFV
                     LRRDYSPNFPLRLMHKDIHLLLDTAKEVRVKLPALETVDEIYELSSEEGWDDLDFAAT
                     IQLLEKWAGLGGQTK"
     gene            complement(2432..3340)
                     /locus_tag="VFC15_07005"
     CDS             complement(2432..3340)
                     /locus_tag="VFC15_07005"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZM09941.1"
                     /translation="MPAVKLALGAIWAPHRFIEVLDDWGDDAFDFLRHQVPRIVAVLV
                     IAFVLSRLLKLVSRHLGELSNRQDLPSSVRSQQLRTLSSVIYSVGVIVIIFIAAMQIL
                     PLLGINMGPLLASAGIAGLAIGFGAQALVKDFINGFFILAENQYDIGDTIRIAGVQGA
                     VENVTLRRTVLRDSDGTVHTVPSSEIKVVSNLTRDWTQINLHVSVAYGADSEQVIGLL
                     KQVGEEMASDPNFGDMIVARPEVAGIEKVSGNEVDYLMLIKTKPGAQYAVRRELRRRI
                     KSSFEKNNIDPGDPSRVFVTGIAKSS"
     gene            3453..3839
                     /locus_tag="VFC15_07010"
     CDS             3453..3839
                     /locus_tag="VFC15_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09942.1"
                     /translation="MNWFVVTRKAFKFNLAGALLLFALLGSSALAQVADPTQTPDITT
                     TGRQRQREAEDQWTLDQKREMIKKQNLQRQQDLKKDTDQLLDLATELKQYVDKTNENI
                     ISLDVIKKAEQIEKLAKSVKEKMKGQ"
     gene            complement(3949..>6325)
                     /locus_tag="VFC15_07015"
     CDS             complement(3949..>6325)
                     /locus_tag="VFC15_07015"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZM09943.1"
                     /translation="SAALAQPTETAGEANLQLPDLSSVTFLGMTGHNLLLIGLLFCVF
                     GLVFGLAIYMQLKNAPVHRSMREISELIYETCKTYLITQGKFILVLWVFIAVIITAYF
                     GWLAPVPGKPIAVTLPVILFFSLVGIAGSYGVAWFGIRVNTFANSRTAFAGLRGKPYP
                     IYAIPLKAGMSIGMMLISVELLIMLCILLFVPADYAGQCFIGFAIGESLGAAALRIAG
                     GIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGV
                     ALITFILLGVKDPHVQVQLLVWIFVMRIMMLVSSTVAYFLNEAVAKARYGNADEMNYE
                     SPLTSLVWLTSIISIVLTYIVSKVIIPDLNGDPTQWWKLATIISCGTLAGAVIPELVK
                     VFTSTSSGHVKEVVTSAEEGGASLDILSGLVAGNFSCYYLGFTMVVLMSIGYVVSTYG
                     LGMLMLAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEQVPNIEAE
                     LKRDYNIDVNFERAKHMLEENDGAGNTFKATAKPVLIGTAVVGATTMIFSIIMALTKG
                     LTENVNKLSLLHAPFVLGLISGGAIIYWFTGASMQAVTTGAYRAVEFIKKNIRLEGVE
                     KASVEDSKKVVEICTKYAQKGMFNIFLAVFFVTLSFAFVEPFFFIGYLIAIAISGLYQ
                     AMFMANAGGAWDNAKKIVEVELKQKGTPLHDATVVGDTVGDPFKDTSSVAMNPIIKFT
                     TLFGLLAVELAVQLTATSGTTFAHILAAIFFLISFTFVWRSFYAMRIVKTL"
BASE COUNT         1436 a         1797 c         1807 g         1285 t
ORIGIN      
        1 tttgaattcc acagctgctc ggtgccgtca aaaggcactc caaccgctgt gcctatcttc
       61 caactccctg ttctctcagc tattctcagc taaactccag gagattctgg gagttgcttg
      121 cctgtaagtt attgaaaaga atggctcctc aggtaggact cgaacctaca accctccggt
      181 taacagccgg atgctctgcc attgagctac tgaggagtgc gtggacgctt cggaatgcgt
      241 catttctgtt gttattgtaa cggcgccatg tcgtggcgtc aaaggtttcg ggcacgctct
      301 cgcgtccgaa tctgtaaact gtaacagtct tctttcatgc ctatgtccag atcacactat
      361 gtgttggtca gaggcttgcc tgattccacg gatgacgtct gatcgctcag gcggcacgaa
      421 ttgcgctccg ctcccactac ggatcgagct tcggccaaaa tgttcgcgag tgctccacca
      481 agaatccacc ggccctgccg ttccgactta tcccgcacct gaacatccgc cgactactcg
      541 aagaacttgg tcacgccttc ttccatgacg cagatcctcg taccaatgcc gtgctgttta
      601 gcacccgcca ataaccgctg cacgggttca tccaccggtt cataggacaa tcgaaatgtg
      661 ccgtagtgca tgggcaccat ccagcgcgcg cccagatcct gaaacgcttg aatggcgtct
      721 tccggactgg tatgaacatt tcgaaacgac gccggatgat acgcgccgat gggcagtagc
      781 gccagctcag ggttgaggcg ctccccgatt tccttgaagc catcgaagta ggcggtgtca
      841 cccgcgtggt acaccgagtt ccggccggcc cgcaacacgt atccgccata gccgcgatga
      901 gtgtcgcgaa ccacacgcgc tccccagtgg cgtgacgggg tgtgcgtgat ctcgacgttt
      961 ccgagttgga agttctgcca ccattccagt tcgaccaccc gtgagaatcc cagatcgccc
     1021 acaaggtcac gcaaattgtt gggcactaca acgatcggtc gcttgccgga cttctggcgg
     1081 gtcaggttcg cgatggtccg gagcgatgga cggtgcaagt gatcgaagtg agcatgcgtg
     1141 accagcaccg catcgatggg tggcagatcg cgcatgcgca gtcctggacg tcggagccgc
     1201 ttcagtacga agatccaacg ggcgaagttc gggtcgacca gtatgttctt gccgccgatt
     1261 tgcagaaaaa aactggagtg cccaatgaac gtaactccga gttgttcccg ggtagccaaa
     1321 accggtttac gactgttgcc gctgcgggga gtcacggccg agcggcctac cagcgtgctg
     1381 aattgccggg ccttgcgcct gatgcttata cctatcatga gatgtcaatg tattagacgc
     1441 cgctggaccg cctggcgatg cgcccgcttg gtgaagagct tccttgacct tctccagcca
     1501 gtcagcttaa atcaaagtgc ggtcataagg agagatgcat gcgaatcgcg tttcttggtt
     1561 tggggatcat ggggcggccg atggcctcga acctggtaaa agcggggcac gaagtttcag
     1621 tatggaaccg gtcggctcgg caacacatcg atggagccag gacggcgatt tctccagccg
     1681 atgcggccca ggatgcagag attgtctgga tgtgcgtggc cgatactgcg gctgtcgaac
     1741 gtctggtgtt ctctgaagac ggtatcgagc aatcgctgcg cgaaggaatg gtgatcgccg
     1801 actccagcac catctcgccg tcagccactt tgcgtttcgc ccaacgcgtc gccgaaaagg
     1861 gcgtgcaatg gatcgacgca cccgtgaccg gctccaaaat cggggccgaa aatggacagc
     1921 ttattttcat tgtgggtggt ccggcggaga cggtccaagc tatggaacct ctgttcaagg
     1981 ccatgggcaa gaccgtaatt cacgtcggcg atactgggaa aggccaggca gctaaaatct
     2041 gcatgaactt gcagatagcg atgatctatg aaggattcgc tgaaggtatg acgttggcca
     2101 gaaaacttgg agtcgccccc gagaagatgg ttgaactggt ccaggcatcg atgatccgct
     2161 cgggtgtggt cgattacaaa gcgcctttcg ttttgcggcg cgactactcg cccaacttcc
     2221 ccctgcggtt gatgcacaaa gatattcact tgctgctcga caccgccaaa gaggtgcgcg
     2281 taaaactgcc agcccttgaa accgtggacg agatctacga gttgtcgagc gaagagggat
     2341 gggacgacct cgacttcgcc gcgaccattc agctcctgga gaagtgggcg ggattgggcg
     2401 gccagacgaa atagcgcatc caaccctgtc gttaactgga ttttgcaata ccagtcacga
     2461 acacccggct gggatcgccg gggtcgatat tgtttttctc gaaactcgat ttgatgcggc
     2521 gccgtagttc gcgccggact gcgtactgcg ccccgggctt ggtcttgatc agcatcaggt
     2581 agtcgacttc gttgccactc accttctcga tgccggcgac ttccgggcgg gcgacgatca
     2641 tgtcgccgaa gttagggtca ctggccatct cttcacccac ctgcttcagc agtccgatca
     2701 cctgttcgct atcggcgcca tacgccacgg aaacgtgcag attgatctga gtccaatcgc
     2761 gcgtgagatt gctgaccact ttgatttcgc tactggggac ggtgtggacg gtgccgtcgg
     2821 aatcgcgcag cacggtccgc cgcagcgtca cgttttccac cgcaccctgc acacccgcga
     2881 tgcggatggt gtctccgatg tcgtattgat tctccgccag gatgaagaag ccgttaatga
     2941 aatccttgac cagtgcctgc gcgccgaatc cgatggcgag gccggcaatc ccggcgctgg
     3001 ccagcagtgg tcccatgttg attcccaaca gaggaaggat ctgcatcgca gcaatgaaga
     3061 tgatgacgat gactccgacg ctgtaaatga cgctcgacag cgtacgaagc tgctgggaac
     3121 ggaccgacga cggcaggtcc tggcgattgc tgagttctcc cagatgtcgc gaaaccagtt
     3181 tgagcagacg gctgaggacg aaagcgatga ccagtaccgc aacaattctt ggaacctggt
     3241 gtcgtaggaa atcgaaggca tcgtcgcccc agtcgtcgag cacctcgata aaccggtgcg
     3301 gggcccaaat ggctcccagg gcaagtttga ccgcaggcat tatcatcgtt ggggcgatta
     3361 tagcaatgcg ttatgagtgc agagatgaag ccaacagatc gtgcgggcca cgggagcatg
     3421 ggttcggcgc agcgtatcag ttcggagatg ctatgaattg gtttgtagtg acgcgaaaag
     3481 ctttcaagtt caacctggcg ggagcgctgc tgctcttcgc gcttcttggc tccagcgcgc
     3541 tggcgcaagt cgccgatcct acccagacgc ccgatatcac cacaaccggc cgtcagcgac
     3601 agcgcgaggc cgaggaccag tggacccttg atcagaaacg cgaaatgatc aagaagcaga
     3661 acctgcagcg ccagcaggac ctcaagaagg ataccgacca actgctcgac ctggccaccg
     3721 aactgaagca gtacgtggac aagaccaacg agaacatcat ctcgcttgac gttatcaaaa
     3781 aagccgaaca gatcgagaaa ttggcgaaaa gcgtgaagga gaaaatgaaa gggcagtaac
     3841 cacaattggc gggcgcgcgt tcgagaagga acttaggcgg aaacttttgt gcaaaagcaa
     3901 aacgccatcc cacgcgggat ggcgtttgaa ttgcctgcga aatgcctgtt aaagggtttt
     3961 cacgatgcgc atggcgtaga acgaccgcca cacaaacgtg aacgagatca agaagaagat
     4021 ggccgccaga atgtgagcga aggtcgtgcc actcgtggcc gtcaactgca ccgcgagttc
     4081 caccgccaac aatccgaaga gcgtggtgaa ctttatgatc ggattcatgg ccaccgatga
     4141 agtgtccttg aacggatcgc ccaccgtgtc gcccaccacc gttgcgtcgt gcagcggtgt
     4201 tcccttctgt ttcagttcga cctcaacaat cttcttggcg ttgtcccagg ccccgccggc
     4261 attggccatg aacatcgcct ggtacaggcc cgagatggca atcgcgatca ggtagccgat
     4321 aaagaagaac ggctccacga aggcaaatga cagggtcacg aagaataccg cgaggaagat
     4381 gttgaacatg cctttctgcg cgtacttggt gcagatttcg accaccttct tgctgtcttc
     4441 caccgaagcc ttctcgacgc cttccagacg gatgttcttc ttgataaact ccaccgctcg
     4501 ataagcgccg gtggtgaccg cctgcatgct ggcgccggta aaccagtaga tgatggcacc
     4561 accgctgatg agtccgagta caaacggagc atgcagcaga gagagtttgt tgacgttctc
     4621 ggtgagcccc ttggtgagcg ccatgatgat ggagaaaatc atggtggtcg cgcccaccac
     4681 ggcggttccg atcagcaccg gtttcgccgt agccttaaag gtgttgcccg cgccgtcatt
     4741 ttcttccagc atgtgcttgg cgcgctcgaa gttgacgtcg atgttgtagt cccgcttcag
     4801 ttcggcctcg atgttcggga cctgctcgat caacgacaat tcatacaccg actgcgcgtt
     4861 gtccgtcacc ggaccgtagg aatcgaccgc gatcgtcacc ggccccatgc cgaggaagcc
     4921 gaacgctacc aggccgaagg cgaacacggc tggggccagc attagcatgc cgagtccata
     4981 agtgctcacg acgtagccaa tgctcatcag gacgaccatg gtgaagccca ggtagtagca
     5041 ggagaagttc ccggccacca gtcccgacag gatgtcgagc gatgcgccgc cttcttccgc
     5101 cgacgtgacc acttccttca cgtgtcccga cgacgtcgaa gtgaatacct ttacgagttc
     5161 aggaataacc gcgcctgcga gcgtaccgca agaaattata gtcgccagct tccaccactg
     5221 cgtgggatcg ccgttaaggt cgggaatgat caccttggaa acgatgtagg tcagcacgat
     5281 ggagatgatc gaagtcagcc acaccaggga tgtcagtggc gattcgtagt tcatctcgtc
     5341 ggcattgcca taacgcgcct tagctactgc ctcgttcagg aagtaagcca cggtgctgga
     5401 aaccagcatc atgatgcgca taacgaaaat ccacaccagc aattgcacct ggacatgagg
     5461 atctttgacg ccgagcagga tgaacgtgat cagcgccacg ccggtcacgc cgtaggtttc
     5521 gaagccgtca gcgctgggac caaccgagtc gccggcattg tcaccggtgc agtcggcgat
     5581 gacgcccggg ttgcgggcat cgtcttcctt aatcttgaag acgatcttca tcaggtcgga
     5641 gccgatgtcg gcgatcttgg taaagatgcc gccggcgata cgcagggcgg ccgcacccaa
     5701 tgactcgcca atcgcgaatc cgatgaagca ctgaccggca taatcggccg gcacgaacag
     5761 caggatacat agcatgatca gcaactcgac gctgatcagc atcatgccaa tactcatgcc
     5821 cgctttcagc ggaatcgcat agatgggata gggtttgccg cgcagtccgg cgaacgccgt
     5881 ccgcgagttt gcaaaggtgt tgacccggat gccgaaccag gccacgccat aactgcccgc
     5941 gatgccgacc aagctgaaga acaggatgac cggcagcgta actgcaatcg gcttccctgg
     6001 gacgggagcc agccacccaa aatacgcagt gatgatgacc gcgatgaaga cccacagaac
     6061 gaggatgaat ttcccctgcg tgatcagata cgttttgcag gtttcgtaaa tcaattcgga
     6121 aatctcgcgc atcgaccggt gcaccggagc gttcttgagc tgcatataaa tcgccagccc
     6181 aaacaccagt ccaaacacgc agaacagcag gccgatcagc agcaggttgt gccccgtcat
     6241 gccgagaaag gtgaccgaac tcagatcggg aagctgcagg ttcgcttcgc cagcggtttc
     6301 agtcggctgg gccaaggcgg ctgag
//
LOCUS       DASRKK010000328         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1100420, whole genome shotgun sequence.
ACCESSION   DASRKK010000328 DASRKK010000000
VERSION     DASRKK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100420"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..632
                     /locus_tag="VFC15_07020"
     CDS             15..632
                     /locus_tag="VFC15_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM09944.1"
                     /translation="MARANSLWRAPRIHGELLKLGIKISERTVSRILRTVKRPPSQSW
                     KTFLQNHLGEIIAIDFFTVPTVRMRVLFVFIVLEHHRRKVLHFGVTEHPTSEWVGQQV
                     VEAFAEREAPRYMIRDRDASYGNEFRRRIQSLGIKEVITAPRSPWQNAFAERLIGSIR
                     RECLDHVVVLNERHLRRLLKKYLAYYHGSRTHLALKKDAPEPRRS"
     gene            727..1065
                     /locus_tag="VFC15_07025"
     CDS             727..1065
                     /locus_tag="VFC15_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09945.1"
                     /translation="MLNRSAIVVKPRQPFLDWLHTADPTSHRLTLRDLTREPTIYLIP
                     ECDTEEDVREVLEELCEEIFVAQLAGWFRNEETWPRDLGFDVFCRWFDFQHHSMLVDL
                     CDEPLIDELD"
     gene            complement(1165..2274)
                     /locus_tag="VFC15_07030"
     CDS             complement(1165..2274)
                     /locus_tag="VFC15_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZM09946.1"
                     /translation="MATKKTGFFPLYTITPAIAKGLMRIEAVRQTVEALPITPRVLAS
                     LRESARLFSTHYSTMIEGNLLTQEQVAQVIGEGQHFPGRERDQSEVLGYYAALDEVEK
                     LAKSQTALTETTVRRLHALVMGSGKTRVKPTPYRDGQNVIQDSASRAIVYMPPEAADV
                     PQLMKDLVAWVTAENDLPVPLKAAIAHYQYATVHPYYDGNGRTARLLTTLILHLGGYG
                     LKGLYALEEYYARDLAAYYRALDVGSSHNYYMGRADSDITKWLAYFIEGMATSFEKVR
                     EQAQREADSGSRDQSRLLRNLDSKQRKVLLLFEKSREVSAKDIADLFGFQQRTAAALC
                     QRWVEDKFLIVTNSSNKARRYRLADSYEESFSKDW"
BASE COUNT          568 a          749 c          776 g          598 t
ORIGIN      
        1 atctgatcta gaccatggct cgggcgaatt ctttgtggcg ggcgccgcgc attcacggcg
       61 agcttctcaa gctcggcatc aagatttccg agcgcaccgt gtctcgcatc ctgcggacgg
      121 tcaaacgccc cccgtcacag agctggaaga cctttctaca gaaccacctg ggggagatca
      181 tcgcgattga cttcttcacc gtacccacag tcaggatgcg cgtactattc gtgttcatcg
      241 tcctggagca ccaccggcgg aaggtgcttc attttggagt cacggaacat ccgacctcgg
      301 aatgggtagg gcagcaggta gtggaggcct tcgccgagag ggaggctccg cggtatatga
      361 tccgtgaccg tgacgctagc tacggcaacg agtttcggcg ccgaattcag tcgctgggga
      421 taaaggaggt gatcaccgca ccccgaagtc cgtggcaaaa cgcttttgcc gagcgactga
      481 ttggctcgat tcgacgggag tgtctggacc atgtcgtggt tttgaatgag cgacacctgc
      541 gcaggttgct gaagaagtat ctggcgtact atcacggttc tcggacacac ttggctttaa
      601 agaaggatgc tcctgaaccg cggcggtcat gaagcaaggc gcgatccttg ccattcccca
      661 ggtgggtgga ctgcaccacc gatatgaacg ccgcgccgcg tgagcgcaga gaactctggg
      721 tgagtcatgc tgaatcgatc ggccattgtg gtgaagccaa ggcagccctt tctggactgg
      781 ctgcacacgg ccgatcccac cagccacaga ctcacgctac gtgacttgac tcgggaaccg
      841 acgatctacc tgatcccgga gtgtgacacg gaggaggacg tccgcgaagt cttggaggaa
      901 ttgtgcgagg agattttcgt ggcgcaattg gcgggttggt tccgaaatga agagacctgg
      961 ccgcgagacc tcgggttcga tgtcttctgc cggtggtttg atttccaaca ccattcaatg
     1021 ctggtggatc tctgcgatga accgctgatc gacgaactcg attgactggt tgggtgtgtc
     1081 gtgcagcccg aaaaacgatg cgatctattt ttggcgaacg acagccgtat agaagcgctc
     1141 gacacaggct ctgagttcgc acaactacca gtccttgctg aaagattcct cgtaggaatc
     1201 agccagccga taccggcgcg ctttgttgga agaattcgtg acgatcagga acttgtcttc
     1261 cacccagcgc tggcataggg ccgcggcggt tcgctgctgg aacccgaaca ggtcggcaat
     1321 atctttcgca ctgacttcgc gcgatttctc gaataggagc aggactttcc tctgctttga
     1381 gtcgaggttg cgcaatagcc gcgactgatc gcggcttcca gagtcggcct cgcgctgtgc
     1441 ctgctcgcgt accttttcaa aggatgtagc cattccttcg atgaagtacg ctaaccattt
     1501 tgtgatgtcg gaatccgctc ggcccatata gtagttgtga gaagagccga cgtcgagcgc
     1561 cctgtaatag gctgccaggt cacgagcgta gtactcctcc agagcataca gacccttgag
     1621 accgtatcct cccagatgca ggatgagcgt ggtcagaaga cgcgcggttc tgccgttgcc
     1681 gtcatagtag ggatgcaccg tcgcgtactg ataatgagca atcgccgctt tcagcggcac
     1741 cggcaaatca ttctctgccg tgacccaggc aaccagatcc ttcatcagct gaggaacatc
     1801 agccgcttcg ggtggcatat agacgatggc tcgggaggcg ctgtcctgga tgacattctg
     1861 cccgtcacgg tatggagtgg gtttcaccct ggttttgccg gagcccatca ccagggcatg
     1921 gagtctccgc acggtcgtct cggtcagcgc cgtctggctt ttcgccagtt tctccacctc
     1981 gtccagtgcg gcgtaatatc cgagaacctc cgactgatcg cgctcccggc caggaaagtg
     2041 ctgcccctcg ccaatcactt gggcgacctg ctcctgagta agcagattgc cctcgatcat
     2101 ggttgagtag tgcgtggaga agagtcgcgc gctctcgcgc aggctggcga gtactcgtgg
     2161 agtgatcgga agcgcctcga ccgtctgccg gacggcctcg atccgcatca acccttttgc
     2221 gattgctggt gtgatcgtgt acaaggggaa aaagccagtc tttttggttg ccatgggcca
     2281 ccctgtcacc gggcatctga cgataatgcg cggttattat gcttttatta taccgataat
     2341 atgctgataa atcagccgcg aaccgtccgt cgtggctctc gcaaaaacca tattgcagat
     2401 aagtaacccg aaaaaagtct ggcgcaaggc tgggctgtga gactgcatga agaggcgagc
     2461 gtccaataat tgctaatttc gccgtaaatg ttcccgtgcc ccgttggact cgatcagatc
     2521 tcaaagaaaa ggccggagcc acaagaaatg gcaggttcac ggcaccctcc agaacctccc
     2581 cctgacgcgg gattttacgc ccgagaatcc attctggcga catgggcgtt tctcgttcct
     2641 aatccggcca gatctcccag aagccggctt ctggacaata tcgggaattc a
//
LOCUS       DASRKK010000329        15357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1295539, whole genome shotgun sequence.
ACCESSION   DASRKK010000329 DASRKK010000000
VERSION     DASRKK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295539"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VFC15_07035"
     CDS             complement(<1..1216)
                     /locus_tag="VFC15_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM09947.1"
                     /translation="MHICLVSTFPPAPCGIATYCGYLIDGLLSVNKKLRITVLAEGPV
                     ANDLRARVNVIEAFSVDSDYGPAILHQVRACAPDVVHIQHEYGIFGFDGRFLHFLADL
                     REIRIPIVVTLHTVHTRSSLDVGCCWRTDRRLPTNVDIESEQRQLCNLGDVIVVHQDC
                     PMRTTLIEQGARADRVVSICHGTFKGTGIISALAKEYLGYAADTSLLVAFGYFEPSKN
                     HQRLLEAFSLLRLRKPNAKLWIGGHIRWPSPPAREYKARIVNLIEQMGLKEHVTLWEE
                     LLSETDVRVLLSAADVGCFAYHEDTYSSSGALHRMLGDGKPIVASHIPKFHELLRVAP
                     EILVDPDRPEEIARVLIRVLEDDSFRTLIASKLLRFAEETSWPLVADSHLDVYRRAIA
                     AVATEPAAGVGPS"
     gene            1381..2490
                     /locus_tag="VFC15_07040"
     CDS             1381..2490
                     /locus_tag="VFC15_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09948.1"
                     /translation="METAEQFVRLSLAQRHQPEGSILLHDLQLDYLRAQWPPEDRNAL
                     QLILGAMRLSSHVIAEDPCQFVSQLVGRLLHYQGKAAIDRLTRSAARGAQQPWLRSLR
                     PALHAPGLGLFRTLSAHRNSVYRVALSGDGRIAIVACSDRTTKVWDVGSGQELRTLSG
                     HGGGVKDLALSGDGRIALSASDDKTLTVWDVGSGRELRILSGHSGDAKSVAMSLDGQT
                     AVSASSEYFLGTTPDKNDQMGLAYSTDLVHWTEATKTPVLPRRPGQFDSRVVEPGPSP
                     ILTPDGIVLIYNGGDDKLVYRTAIAIFDRNDPRKLLYRSEQPIFAPEKEWEKVGQVPN
                     VVFVEGVVRRGERYLFYYGGADKYVGVAEAPVVSH"
     gene            2576..3643
                     /locus_tag="VFC15_07045"
     CDS             2576..3643
                     /locus_tag="VFC15_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09949.1"
                     /translation="MGRSHTSAVKWLSVFLLAGCTLLQAQTLSPTNTVSSPQAAKSSG
                     SDTDFAEARKLSQQGKYDEAIAMLRPMAARTPRPAGISKELGTVYYKKGDYLKAADSL
                     KQAAADDPNDKEAVQLLGLSYYLAGRPAEAIPYLEKVQAWYPRANVDAAYVLGVCYIQ
                     AQDYPQARRAFAKMFDVSPDSGASYLITARMLFRQEFIPIAEDYSQKAIAADPKLPLA
                     HQLLGEIHLFTSKIPEAINDFQQELAINPAYPPALYKLADAYSRVQKYDEAERLLQRS
                     IWLDASSTGPYILMGKVLQKKGEPVLAIRTLQRAATMDPNNPITHHLLGQAYRDIGKN
                     DEAEQELKIAEQLRGKQNGSD"
     gene            complement(3640..5313)
                     /locus_tag="VFC15_07050"
     CDS             complement(3640..5313)
                     /locus_tag="VFC15_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZM09950.1"
                     /translation="MTLFRGLSPAALAILLVMTSAGFAAQSAGSQVAKPDANGYVGNE
                     ACAKCHAAIFESYKNTAMAHASGPATENLIAGDFTHHASGVNFRVYSDAGKVWLSFDR
                     PGDPAVHGKRELLYYIGQGRRGRSYLFSDDGFVFESPVNWYTDRHMWDMAPAYGNARE
                     IPMTLPALTSCLDCHVSGIQPPINGTENRYKMPVFAYSGVTCERCHGPGAAHPNGGAI
                     VNPTKLSANRRDQVCMQCHLEGNAAIERVGKHLYEYRPGDDLPDYIRYYLLVDSHSPN
                     LRAASQFEALAQSTCKKKSGDAMSCTSCHDPHRSIPADERVSFYRGKCLVCHGAAFGA
                     KHHVDQPDCAHCHMPAALSSDVAHTEVTDHRIPRRPGTGPQLENAGPPVSPQLVPFPS
                     SKDTEHDVRDLALAWQSIVNSGMKVEQPQAEKLLRQAATQFPDDPVVLSALGYVEQTR
                     GANDRAQDLYRKALALDPNLIDAATNLGVLEAQRGHLAEAVKLWQGAFQRAPGRSSIG
                     MNLARAFCAAGQAKVARSYALRVLQFNPDLSAARKLLNGIDADPPKCAP"
     gene            complement(5434..7212)
                     /locus_tag="VFC15_07055"
     CDS             complement(5434..7212)
                     /locus_tag="VFC15_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM09951.1"
                     /translation="MSMCSGAMLWAQNSTLPKEKATFNRDIAPIVYQYCAACHRPGEA
                     GPFSLLTYQDVKSHGHQIMAVTHTRFMPPWPPEPGGLKLADERRLSDEQIATIKVWVD
                     EGMLEGKAVDLPPKPKFVEGWQLGEPDLILKADKPYIVPASGGDQYWNFILRVPINET
                     RWVRAVEIRPGDKRLVHHANMLVDRNQSARRMERDPGSGFGGMEVRIESEIFDPDSHF
                     LFWKPGTVVSNEPDGMALRLDKGTDLVLNTHLQPSGKPEAIQPSVGLYFTDKPATLHP
                     MLLEMQNDGALDIPAGAKDFVVTDEFKLPIDVDLIGIYPHAHYLGKDILATAELPDGT
                     SKTLIHIKRWDLNWQAVYRYAEPVPLPRGTTITMRYSYDNSEDNAANPNHPPARVIGG
                     NRSSDEMAHLWLQVLPKGDPPDGVDPRMVLQEAWARHEVEKAPTIYEAHYNLGSMLLQ
                     RGALDEAIVQFQAAASIRPSDPVANNALGGALLAKGDLDGAVQRFKSALAAQPDYFDA
                     HYNLASVLASQGNFQDAASQFGEAVRLNPEDANAQANLGTALAQLGRLTEAKSHYEAA
                     LRIDPGNQLARENLQQIEQMTNQAPH"
     gene            complement(7272..7898)
                     /locus_tag="VFC15_07060"
     CDS             complement(7272..7898)
                     /locus_tag="VFC15_07060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM09952.1"
                     /translation="MLRRLMGLAASVLAATLLHAAGSPEAALLAPIWEIAPPASTANS
                     AFAQDLSGNAVDPFQVAAGKPLVLIFVRTDCPISNRYAPTIQALGQKYSGVAVMMLVY
                     PDKAETSANIKKQLQEYGYNLAALQDPQHSLVKLSRVEITPEVAVFNSKQELIYHGRI
                     DNWYKEFGRARSAPTTHELDDAIQAALSGNRAAPTSVGGVGCYISDLK"
     gene            complement(7958..9664)
                     /locus_tag="VFC15_07065"
     CDS             complement(7958..9664)
                     /locus_tag="VFC15_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HZM09953.1"
                     /translation="MRQRFHSLVVLLLSFFVVLPFVSAQSKPVDAEGHQWWQHAVFQE
                     IYPRSYMDSNNDGIGDLNGIASKMEYLKWLGVDAIWIAPCFPSPQVDFGYDVSDYENI
                     DPMYGTLADFDKMEKIGKTNGVGIVFDFVVNHTSDKHKWFIESSSSKTNPYHDWYIWK
                     DPKGTNQPPNNWTSTFGGSAWQWNPKLNQFYYHYFYPQQPDLNWRNPKVANAMYDSTR
                     FWYKRGVVGFRLDAVDTLFEDPNLTDNPALPGKDEYGDQNQERKYNDKQPEVHEVMKG
                     LRKAADQYNAVLIGETWTSDVSELKDYYGTKHDELQMPMDLMFTELKPLSADVFRKHV
                     GAVDASGEWPVYVMSNHDIKRAYSRFADGKHDDDIAKVMAGMYLTLRGSAIMYYGEEI
                     GMANNDPTRKEDVKDPIGQLGWPEQKGRDGERTPMQWNTAPNAGFTTGIPWLPVPMTY
                     ETHNVESEKKDPNSILNFYKKLLALRHSNPALLDGDYVALNQDDANVYSYLRRYKGQA
                     VLVVLNMSETPKNVSFDLSKQGFSNGEARTLLSTINGGAAQKLSRISLPPYTVYIGEV
                     TK"
     gene            9850..11655
                     /locus_tag="VFC15_07070"
     CDS             9850..11655
                     /locus_tag="VFC15_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM09954.1"
                     /translation="MSKSRRRFLESLCRSALVLSFSDVLSLVQPLPVFGQETPAANSG
                     GDLGVAFIDVAKEAGLNAKTIFGGEHKNKYLLETTGCGVAFYDYDNDGWLDVFLVNGW
                     RLEGFPPGEEPSNHLFKNNRDGTFTDVTLKAGLKHTGWGQGVCIGDYDNDGNDDLFIT
                     YFGKNVLYHNNGDGTFTDVSEKSGVAGMKTRWATGCAFLDYDRDGKLDLFVANYIDLD
                     LKTAPVPESGPCLYKAVMVACGPPGLNGDSNILYHNNGDGTFTDVSDSSGIKKVNGTY
                     GLGVLVGDFDNDGWPDIYVADDSTQSLMYQNQKSGRFVEVGLESGCVLSADGKPQAGM
                     GVSAADYDLDGNLDILKTNFAGDTPSLYRNQGHWSFEDATFQAGLGLHTKYLGWGCGF
                     FDFDNDGWPDILICNGHVYPEVEQLTTEAGYPQTKLLYKNQHNGHFQEVTANAGPGIT
                     VPNASRGCAFGDFDNDGDVDFVVNTVNGLPQLVRCDSKLDNNWIKVKCIGVKSNRSAI
                     GARVKCVTHPADEPKPHQQIDEVRSGGGYISQNDLRIHFGLGKAEKVEVLEVRWPSGQ
                     VDTMKDVKANQLVTVREGQGIVKSEQLAGAKKAKH"
     gene            complement(11683..13449)
                     /locus_tag="VFC15_07075"
     CDS             complement(11683..13449)
                     /locus_tag="VFC15_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM09955.1"
                     /translation="MYRLPCQAYFFRAMARVPVISVILMALATFATAQSTKNSSQSIA
                     NFVDIAEKAGLTMKNVFGGVDTKKYIIETTGTGVAIFDYDNDGWPDIFFVNGTTLEDM
                     KGGKSGPTNHLYHNNHDGTFTDVTEKAGLLDATGWGQGVCVGDYDNDGWEDLYVTYYG
                     KNRLYHNDHGVFKLVSEKAGVAGNGKEWGAGCAFVDYDRDGHLDLFVANYVDFDVTTA
                     PAPGDRPSCMWKGAPVMCGPRGLPHHKNILYHNRGDGTFEDVTVKAHIDQTNGHYGLG
                     VSTLDYDNDGWPDIYVACDSTPSILYHNNKDGTFTDVAVVAGAAFNEDGREQGGMGTT
                     VADYDGDGRLDIFKTNFSDDTSSLYHNNPDGTFTDVTTDAGLGLYTKYLGWGTMFFDF
                     DNDSWPDLLLVNGHVYPEVDKFHLGSNYQEPRLLYRNNGNATFSDVSDVAGSGITAMA
                     SSRGLAVGDLWNTGQMSAVISTMNDRPSLLKNEQKYPNHWIAFKTVGTKSNRDGIGAR
                     ITVRSGKRLLVDEVRSGSSYYSQSDMRVHFGLGAANKLDSVEIRWPSGLTERFDNAAI
                     DQINTVKEGTGAAVSTAPAARP"
     gene            complement(13507..14415)
                     /locus_tag="VFC15_07080"
     CDS             complement(13507..14415)
                     /locus_tag="VFC15_07080"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HZM09956.1"
                     /translation="MSLGEAFLRNASDARVSGGPMFLMCPPTLYDVNYVINPWMEGNV
                     HCSSRQNAERQWEQLRAALVGLARVELVEPQAGSPDMVFTANAGLVRKRVVTLSRFLH
                     PERQGEEPHFRKWFSDSGFAVRELPRAVPFEGEGDALFEADNSRLWAAHGMRSREASH
                     PYLEDTWGVEVISLRLVDPRFYHLDTCFCPLFGGYVLYFPEAFDAVSQARIAARYPEE
                     QRIAVSLQDALRFACNAINVGRTILLNEVSPQLSGRLESLGFRVIQVPLSEFLKAGGA
                     AKCLVLRLSEMEATHGKSESCRISAA"
     gene            complement(14546..15121)
                     /locus_tag="VFC15_07085"
     CDS             complement(14546..15121)
                     /locus_tag="VFC15_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZM09957.1"
                     /translation="MKRRARFCAIRFLLVLIVLCAGAQAEKIRVHYDKSVDFSKYKTY
                     GWAPIGAVAHPMLALDVVGAIEGELNARGLKKVPANPDLIVQVYGAVDTEVSMTSNNP
                     IYNATGGIPPFDPSMTSPGNSLYWDGYYGNSTVLVHPGQLIIDLIDAKAKKLVWRGMG
                     SEAISPNNPDKLMEEANSTVTKLFNDYPVKK"
BASE COUNT         3163 a         4460 c         4445 g         3289 t
ORIGIN      
        1 tgctgggtcc tacacccgca gcaggttctg tcgcgaccgc cgcgattgct cgacggtaca
       61 catcgagatg agaatccgct accagaggcc aactcgtctc ttcggcaaac cggaggagct
      121 tgctagcgat cagtgtacga aaggagtcgt cctcgagcac acggatcaga acccgcgcaa
      181 tttcctccgg gcgatctggg tccaccagga tttccggtgc gacccggagt aactcgtgga
      241 acttgggaat gtgcgaagcg acaatcggct tcccgtcccc cagcatgcga tgaagcgcgc
      301 cgctggaaga gtacgtgtcc tcatgatagg cgaagcagcc gacgtcggcg gcggaaagca
      361 gcacccgcac gtcggtttcc gaaaggagct cttcccacag agtgacatgc tctttcagcc
      421 ccatttgttc gatgaggttg acgatccgtg ccttgtactc ccgcgccggt ggagacggcc
      481 agcgaatatg cccaccgatc caaagctttg cgttgggctt ccggagacgt agcagggaga
      541 aagcctcaag aaggcgctga tggttcttgg agggctcgaa gtagccgaaa gccactaaaa
      601 gggaggtgtc ggctgcatag cccagatatt ctttggctag agcgctgata ataccagtcc
      661 ctttgaacgt gccatgacag atggacacga ctcggtctgc ccgcgcaccc tgctcaataa
      721 gcgtggtccg catcgggcaa tcttgatgca ccacgatcac gtcgccgagg ttgcacaact
      781 gccgctgctc gctttcgatg tcaacgttag tcgggagcct ccggtccgtt ctccagcagc
      841 agcccacgtc caggctggat cgcgtatgaa ctgtgtgcag cgtaacgaca atgggaatac
      901 gaatttcgcg aaggtcagct aggaagtgca gaaagcggcc atcaaatccg aagatgccgt
      961 actcgtgctg gatatggacc acgtccggtg cacacgcacg cacttggtgg agaatcgcgg
     1021 ggccgtaatc ggagtctacc gagaacgcct cgatcacgtt cacacgtgcg cggagatcgt
     1081 tggcaaccgg accttcggcc agcacggtaa tgcgtagctt cttgttgaca gacagtaagc
     1141 cgtcaatcag atagccgcaa taggtagcaa tcccacaggg agccggcgga aaagtcgaaa
     1201 ctaggcagat gtgcatagca agatcggtgc ggaagtgatt ataccgcagc ctgctaaggc
     1261 ttcagccctg cagaccttcc gcctcagcag gaattggccg gataagtcgg ttccgtcgtg
     1321 ttatggatct tggggagctc tcatcagcag tgtatctggg gagtggatga aagcgaggca
     1381 gtggaaaccg cggagcaatt cgttcgactc tcgctggcgc agcgtcatca gcccgaaggg
     1441 agcatccttc tgcacgactt gcagctcgat tatttgcgcg cccagtggcc gcccgaggat
     1501 aggaatgccc ttcaactcat actcggagct atgcgcctct cttctcacgt gatcgccgaa
     1561 gatccatgcc agtttgtctc gcaattggtc ggccgactct tgcactatca gggtaaggca
     1621 gcgattgatc ggttgacccg cagtgcagcg agaggtgcgc aacaaccgtg gctccgatcg
     1681 ttgcggcccg cgctacatgc tccgggtttg gggctttttc gcactctctc agcccacagg
     1741 aattcggtct accgcgtggc gttgagtggc gacggacgga ttgctatcgt cgcgtgttcg
     1801 gaccgtacga cgaaggtctg ggatgtgggg agtgggcagg agctgcgtac cctcagcggc
     1861 cacggcggag gagtcaaaga cttggcgttg agtggagatg ggcggattgc gctctctgcg
     1921 tctgatgaca agacgctgac ggtgtgggac gtggggagcg ggcgcgagct gcgcatcctc
     1981 agcggccact ctggtgacgc caagtcggtg gcgatgagtc tagacgggca gactgcggtg
     2041 tccgcgtcta gtgagtattt tctcggtacg actcccgata agaatgacca gatgggattg
     2101 gcatactcga ccgacctcgt gcactggacc gaagcgacga agacgcctgt attgccgcgc
     2161 cgtccgggac aattcgactc ccgcgttgtc gagcctggac catctccgat cctgacgccc
     2221 gacggcattg tgctgatcta caacggcggc gatgacaagc tggtttaccg cactgcgatc
     2281 gcaatcttcg accgcaacga cccgcgcaag ctgctctatc gcagcgagca gccgatcttc
     2341 gcgcccgaga aggaatggga gaaagtcggc caggttccga acgtggtttt cgttgagggc
     2401 gtggtccgac gcggtgagcg ctacctgttt tattacggtg gcgcagacaa atatgttggg
     2461 gtggcggaag cgccggtcgt aagtcactga gcttcaattg gctaaattca atccgcagac
     2521 ctcgtaattg agccgctccc cgtgtcgttg tgttctaatt ttgtcgatgg cctcaatggg
     2581 acgctcgcac acctcagctg ttaagtggct ttctgtattt ctgctggctg gatgcaccct
     2641 gctgcaagcg cagacgctgt ctccgaccaa cacggtctcg tctccgcagg cggcgaagtc
     2701 ttccggctct gacaccgact tcgccgaggc ccgcaaactt tcccaacagg gcaagtatga
     2761 cgaggccatc gcaatgttgc ggccaatggc cgctcggacg cctaggccgg ctggcatttc
     2821 gaaagaattg ggcacggttt attacaagaa gggtgactat ctgaaggctg cggactccct
     2881 caagcaggca gcggctgacg atcccaatga caaggaagcg gtgcagttgt tgggtctgtc
     2941 ctactacctc gctggacgtc ctgcagaagc aattccgtac ctggaaaaag tccaggcatg
     3001 gtacccgcgg gccaatgtcg atgcggctta cgtacttggc gtctgttaca tccaggcgca
     3061 agattatccc caggcacgca gggccttcgc gaagatgttc gacgtttcgc ccgactctgg
     3121 ggccagctac ctgattacgg cgcgcatgtt attccgtcag gaattcattc cgatagcgga
     3181 ggactactca caaaaagcta tcgctgccga tcccaaattg ccccttgccc accagttgct
     3241 gggcgagatt catctattca catcgaagat tccagaagcg atcaacgact ttcagcagga
     3301 gttggcgatc aatccagcat atcctccggc gctctacaag ctggccgatg cgtactctcg
     3361 ggtgcagaaa tatgacgaag ccgagcggtt gcttcagcga tctatttggc tggacgccag
     3421 ttctaccggc ccatacatcc tgatgggcaa agttctgcag aagaaaggcg aacccgtgct
     3481 cgctattcgt acgttgcaac gtgcggcgac gatggaccca aacaacccca tcacgcacca
     3541 tcttctcggg caggcttacc gggatatcgg caagaacgac gaggcggaac aggagctgaa
     3601 aatcgccgaa cagttgcgcg gcaagcagaa cggtagcgat tagggggcgc actttggcgg
     3661 atcggcatct atcccattca acaactttct tgctgcgctt aaatcgggat tgaactgcag
     3721 cacccggagt gcgtagctgc gcgcaacttt tgcttgcccg gcggcacaaa atgcccgcgc
     3781 caggttcatc ccgatgctgc tgcgtcccgg cgcacgctga aatgctccct gccatagctt
     3841 tacggcctcg gctagatgtc cgcgttgcgc ctcaagcaca ccaaggttcg tagccgcgtc
     3901 gatcagattc ggatcgagtg ccagcgcttt tcgatacaga tcctgcgcac gatcgttggc
     3961 tccccgcgtt tgttcgacat aacccaacgc cgacaggacg accgggtcat cgggaaactg
     4021 cgtcgccgcc tggcgcagta acttctccgc ctgtggctgc tccactttca tcccactgtt
     4081 gacaatcgat tgccacgcca acgcaaggtc gcgaacatcg tgttccgtgt ccttcgagct
     4141 ggggaagggc accagttgcg gtgaaacggg cggcccggca ttctccaact gcgggccggt
     4201 gccggggcga cgcggaatgc gatgatcagt cacctcggtg tgcgcgacat cgctgctgag
     4261 ggcggcgggc atgtggcaat gtgcgcagtc cggctgatcg acgtggtgct tcgctccgaa
     4321 cgccgctccg tgacagacca ggcacttacc gcgataaaag gacactcgct cgtcagccgg
     4381 aattgaacga tgcggatcgt gacaactggt gcacgacatc gcgtcgcccg atttcttctt
     4441 gcacgtgctc tgcgcgagcg cttcgaactg gctcgctgcc cgcaaattcg gagagtgtga
     4501 atccaccagc aggtagtagc gaatgtagtc gggaagatcg tcgccgggcc gatactcata
     4561 caggtgcttg ccgacgcgtt cgatcgccgc attaccttcg aggtgacatt gcatgcatac
     4621 ctggtcgcgc cggttggcag acagctttgt cggattgaca atcgctccgc cattcgggtg
     4681 tgcggcacca ggcccatgac agcgctcgca ggtgacgccc gaataggcaa acactggcat
     4741 tttgtagcga ttctcagtgc cgttgattgg gggctgaatt ccgctgacgt ggcagtcgag
     4801 acaactggtc agcgcaggca gcgtcatggg aatttcgcgc gcgttgccgt aggcgggcgc
     4861 catgtcccac atgtgccggt cggtgtacca gttaacgggt gactcgaaga cgaagccgtc
     4921 gtcggagaat agataagacc gtcccctgcg cccctgtccg atgtaataca ggagctcgcg
     4981 ttttccgtgt accgcggggt cgccgggccg gtcgaagctg agccagacct taccggcatc
     5041 gctgtatacg cgaaaattca cacccgaagc atggtgcgtg aaatctccgg cgatcagatt
     5101 ttcggtcgca gggccgctgg catgtgccat ggccgtgttc ttgtatgact cgaagatggc
     5161 cgcgtggcat ttggcacacg cctcgttccc gacgtatccg tttgcatccg gttttgcgac
     5221 ttgcgaacct gcagattggg cagcgaaacc ggcggaggtc atgaccagca atattgccag
     5281 cgccgctggg cttagtcccc tgaacaatgt cacctcgaac tctcccggcc ccatagcgaa
     5341 cgtccagtct tgcgttggta cttcaccaat gtaaaccgag tctgactgag gtggcccacc
     5401 agacgcaggg atttcgctac tgaaccacca cggtcagtgg ggggcttggt tcgtcatctg
     5461 ttcaatctgt tgcaggttct cgcgcgccaa ctgattgccg ggatcgatgc gcagggcggc
     5521 ttcatagtgc gatttcgcct cggttagacg tccaagttgc gccagcgcag ttccgaggtt
     5581 cgcctgggca ttcgcgtcct cgggattcag gcggacggct tcgccgaact ggctggctgc
     5641 gtcctggaaa ttgccttgcg aggcgagcac actggccaag ttgtagtgcg cgtcgaagta
     5701 gtcgggttgc gccgcgagtg cggacttaaa acgttgtacg gctccgtcca ggtcgccctt
     5761 ggccaaaagc gcacctccaa gtgcattatt ggcgacggga tcactcgggc gaatgctcgc
     5821 ggcggcctga aactgcacga ttgcctcatc cagtgcgccg cgctgtagca gcatcgaccc
     5881 caggttgtag tgagcctcat agatagttgg agccttttcc acctcatgcc tcgcccaggc
     5941 ttcctgcagc accatgcgcg ggtctacccc gtcgggaggg tcgcccttgg gcaacacttg
     6001 caaccacagg tgggccatct catcgctcga ccggtttcct ccgatgacac gcgctggcgg
     6061 atgattggga ttggccgcgt tgtcctcaga attgtcgtag ctgtagcgca tcgtgatcgt
     6121 cgtacccctg ggaagcggca ccggctcggc gtaacgatag acggcctgcc agttcaggtc
     6181 ccagcgcttg atgtggatca gcgtcttgct ggttccatcg ggcaactcgg cggtggcaag
     6241 gatgtctttg ccaagatagt gtgcgtgggg gtagatgccg atgaggtcaa catcaatcgg
     6301 gagtttgaac tcgtcagtga ccacaaagtc cttggctccg gctggaatgt cgagcgcgcc
     6361 atcgttttgc atctccagca gcatgggatg cagcgtcgca ggtttgtcgg tgaaatacag
     6421 gccaacgctc ggttggatgg cttcgggttt gccggatggc tggagatgcg tgttgagcac
     6481 caaatcagtg cccttgtcga ggcgtaacgc catgccgtcg ggctcattcg acacgaccgt
     6541 ccccggcttc cagaacaaaa agtggctatc gggatcgaag atttccgatt cgattctgac
     6601 ttccattccg ccgaagccac tgcccggatc tctttccatg cgacgcgcag attgatttcg
     6661 gtcaaccaac atattggcgt gatgcaccag tcgcttgtca cccggacgga tctcgacggc
     6721 gcgcacccat cgcgtttcat tgatcgggac ccgcagtatg aagttccagt attgatcgcc
     6781 gccagacgcg ggcacaatat agggcttgtc cgccttcaga atcaggtcag gctctcccag
     6841 ttgccaacct tcaacaaact ttggcttggg aggaaggtcg acggctttgc cctcgagcat
     6901 tccctcgtcg acccagactt tgatggtcgc aatctgctcg tcggacagcc gccgctcgtc
     6961 cgcgagtttc agccctcccg gctcgggtgg ccaaggaggc atgaaacgcg tatgtgtgac
     7021 cgccatgatt tgatggccat gcgacttgac gtcctgataa gtcagcaggg aaaatgggcc
     7081 ggcttcaccc ggtcgatggc acgcggcgca gtactgatac acgatgggcg cgatgtcgcg
     7141 attgaatgtg gctttttcct tgggaagcgt agagttctgc gcccagagca tcgcgccgct
     7201 gcacatgctc atcgcaatta cagcagatgc aagggcgagg ccgcgtaaca aatggatagg
     7261 acagcggaga ttcatttcag gtccgaaatg tagcaaccca ctccgccgac agaagtaggc
     7321 gcagcgcggt tgccgctgag cgcagcctgg atcgcatcat caagctcgtg cgtggtggga
     7381 gcgctacggg cgcggccgaa ctccttatac caattgtcga tgcggccgtg gtagatgagt
     7441 tcctgcttgc tattgaagac cgcaacttcg ggcgtgattt cgactctact gagtttaacc
     7501 aaggagtgct gcgggtcctg cagcgcggcg agattgtagc catactcctg tagctgcttc
     7561 ttgatgtttg cgctggtctc ggccttatcg ggatagacca gcatcatcac cgcaacgccc
     7621 gaatacttct gcccgagtgc ttggatggtg ggagcgtaac gattggagat cggacagtcc
     7681 gttcgaacaa aaatgaggac cagcggcttc cctgccgcta cctgaaacgg atcgacagca
     7741 ttcccagaaa gatcctgagc gaacgcggag tttgccgtac tggcaggtgg agcgatctcc
     7801 cagataggag ccagcaacgc ggcctcggga ctgcccgcgg catgcagcag agtcgctgcc
     7861 aacacgctgg cggccagtcc catgagtcgc cttaacactt catcgtctcc gaatcatgct
     7921 tgcaggttcg tcccgctact tctcgatttc tcaatcctta cttggtcact tcgccgatgt
     7981 acacggtgta cggcggaagc gaaattctgg aaagcttctg ggccgccccg ccgttgatcg
     8041 tgctgagcag cgtcctggcc tcgccattgc tgaatccctg cttcgaaaga tcgaaactga
     8101 cattcttcgg ggtttccgac atgttcagta ccaccagcac agcttggcct ttatagcgcc
     8161 ggagatacga gtacacattg gcatcatcct ggttgagcgc gacgtaatcg ccgtccagca
     8221 acgccgggtt ggaatgacgc agcgccagca gcttcttgta gaagttcagg atcgagttcg
     8281 ggtccttctt ctcgctctcc acgttgtgtg tctcgtacgt catcggaacc ggcagccacg
     8341 gaatgcctgt cgtgaaaccg gcgttgggtg ccgtattcca ttgcattgga gtgcgctcgc
     8401 cgtcgcggcc tttctgctct ggccagccca actggccgat gggatccttc acgtcctctt
     8461 tgcgcgtggg atcgttgttc gccatcccga tctcttcgcc gtagtacatg atggccgagc
     8521 cgcgcagagt gaggtacatg ccggccatga ccttggcgat gtcgtcatcg tgcttgccat
     8581 cggcgaaacg gctataggct cgtttgatgt cgtgattgct catcacatac actggccatt
     8641 ctcccgatgc gtctaccgca ccgacgtgct tgcggaagac atcagccgat aacggcttca
     8701 actcagtaaa catcaggtcc atcggcatct ggagctcatc gtgcttcgtg ccgtaatagt
     8761 ccttcagttc tgagacgtcg gatgtccagg tctcgccaat caggacggcg ttgtactggt
     8821 ctgcggcctt ccgcaggccc ttcatcacct cgtgcacctc gggctgcttg tcgttgtact
     8881 tgcgttcctg gttctgatcg ccgtactcgt ccttgcccgg aagcgccggg ttgtcagtga
     8941 ggttcgggtc ttcgaaaagc gtatcaaccg catccagacg gaaacccacc acgccccgct
     9001 tgtaccagaa gcgcgtggaa tcgtacatgg cgttcgcaac cttggggttg cgccaattca
     9061 aatccggttg ttgcgggtag aaatagtgat aatagaactg attcaacttc ggattccatt
     9121 gccacgccga tccaccgaat gtcgaagtcc agttattggg tggctggttc gtccccttag
     9181 ggtctttcca gatataccag tcgtgatagg ggttggtctt tgacgacgat gactcgatga
     9241 accacttgtg cttatcggag gtgtggttga ccacgaagtc aaacacaatt ccgaccccat
     9301 tcgtcttgcc aatcttctcc atcttgtcga agtcagcaag cgtgccgtac attggatcga
     9361 tgttctcgta gtccgaaaca tcgtaaccaa aatccacttg cggcgacggg aagcagggcg
     9421 cgatccatat cgcgtccacg cccagccact tcaggtattc cattttcgac gcgataccgt
     9481 tcaggtctcc gatgccatcg ttgttgctgt ccatatagct gcgcggatag atttcctgaa
     9541 acaccgcatg ctgccaccat tggtggcctt cggcgtcgac cggcttgctt tgcgcagaga
     9601 cgaagggaag aacaacgaag aagctgagca gcaggactac cagactatgg aaccgttgac
     9661 gcatcgcacg tgcctccctg gacggtcgtt gatggtatcc gaaaccatac gagtctaatt
     9721 catcgtcttg acggatgcca gaaaccaggg cccttggcac cgttcgcatc agcttgcaat
     9781 tcatgacatc cgtaccggaa aatgagagtt cgttctttgc acgggttaca gcaaggcggg
     9841 tattttcgtt tgtcaaaatc gcgccgtaga ttcctcgagt cactctgccg ctccgctctc
     9901 gtgctctcgt tttccgatgt gctctctctc gtccaaccgc taccagtttt cggacaggaa
     9961 accccagccg caaattctgg cggcgatctc ggcgtcgcct tcatcgacgt agccaaagaa
    10021 gccgggctca acgcaaagac catcttcggc ggtgagcata agaacaagta cctgctggaa
    10081 accaccggct gcggcgttgc cttctacgac tatgacaacg atggctggct cgacgttttc
    10141 ctggtcaacg gctggaggct ggagggcttt ccgccgggcg aagaaccctc caatcacttg
    10201 ttcaagaaca accgcgatgg cacctttacc gacgtcactc tgaaggctgg actcaagcac
    10261 acaggctggg gacagggcgt ctgcatcggc gactacgaca acgacggcaa cgacgacttg
    10321 ttcatcacct acttcggtaa gaacgtgctc taccacaaca atggagacgg cacctttacc
    10381 gacgtgagtg agaagtcggg cgtcgcgggc atgaagacgc ggtgggccac cgggtgcgct
    10441 ttcctcgatt acgaccgcga tggcaaactc gacctcttcg tcgccaacta catcgacctc
    10501 gacctcaaaa ctgcgccggt tcccgagagc ggtccgtgtc tctacaaggc cgtcatggtg
    10561 gcctgcggcc ctccgggtct caacggcgac tcgaacatcc tgtaccacaa caatggagat
    10621 ggcaccttca ctgatgtttc cgatagttcc ggcatcaaga aggtcaacgg aacctacgga
    10681 ctcggcgtac tggtcggcga tttcgacaat gacggctggc ccgatatcta tgtcgccgac
    10741 gattccaccc aatccctgat gtaccagaac cagaagagtg gaagattcgt tgaggtgggg
    10801 ttggaatcag gctgcgtgct cagcgccgac ggcaaaccgc aagcgggcat gggagtctcg
    10861 gctgcggact acgacctcga cggcaatctg gatatcctca agaccaattt tgccggtgac
    10921 actccttcgc tgtatcgcaa ccagggccac tggagtttcg aagacgccac ttttcaagcc
    10981 ggcctgggcc tgcacacgaa gtacctgggt tggggctgcg gtttcttcga tttcgacaac
    11041 gatggctggc ccgacattct catctgcaac ggccatgttt atcctgaagt cgaacaattg
    11101 accacggaag ccggataccc gcagactaag ctgctctaca aaaaccagca caacggtcat
    11161 tttcaggaag tcaccgccaa cgccggccct ggaatcacag tgccgaacgc ttcgcgaggc
    11221 tgcgcctttg gcgatttcga caacgatggc gatgtcgatt tcgtggtgaa taccgtgaac
    11281 gggctgcctc agttggtgcg ctgtgattcg aagctcgaca acaactggat caaagtgaag
    11341 tgcatcggcg taaagtcaaa ccgcagcgcc atcggcgcac gggtgaagtg cgtgacccat
    11401 cctgccgatg agccgaagcc gcaccagcag attgacgagg tccgcagcgg tggaggctac
    11461 atctcgcaaa acgatttgcg gatccacttc ggactcggca aagccgagaa ggtcgaagtg
    11521 ctcgaagtgc gctggcccag cgggcaggtg gacacgatga aagacgtgaa ggcgaatcag
    11581 ctcgtcaccg tcagagaagg ccagggcatc gtgaagagcg agcagttagc cggtgcgaag
    11641 aaggcgaagc actgatggtt gctcagggac tgcggttgac ggctaaggtc tcgctgctgg
    11701 cgcagtactc accgcagcgc ccgtcccttc cttcacggtg ttgatctggt cgatggcagc
    11761 gttgtcgaag cgttcggtca atccgcttgg ccagcggatc tcgaccgaat ccagcttgtt
    11821 ggccgcgccc aacccaaaat gcacgcgcat gtcgctttgc gaatagtagc ttgacccgct
    11881 gcgcacttcg tcaaccagca gccgcttccc tgaccgcacc gtgatacgcg ctccgatgcc
    11941 atcgcgattg gatttggttc ccacggtctt gaaggcaatc caatggttgg gatacttctg
    12001 ctcattctta agcaggctgg gtcgatcgtt catggtgctg atcacggctg acatctggcc
    12061 ggtgttccac aagtctccta cggccagccc acgcgacgaa gccatcgccg tgattcccga
    12121 acccgccacg tcggacacgt cgctaaaggt cgcattgccg ttgttacggt acaaaagccg
    12181 cggctcctgg taattgctgc ccaggtgaaa cttgtcgacc tcaggataga cgtggccatt
    12241 caccaatagc aggtcgggcc aactgtcgtt gtcaaagtcg aagaacatgg tgccccatcc
    12301 cagatacttg gtatagaggc ccagccccgc atcagtcgtc acatctgtaa aagtgccgtc
    12361 cggattgttg tgatacagcg acgacgtgtc gtcggagaaa ttggtcttga agatgtcgag
    12421 ccggccgtct ccgtcatagt ccgcaacggt ggtacccatg ccgccctgct cgcggccgtc
    12481 ctcattgaat gccgcacccg ccaccacggc gacgtcagta aatgttccgt ccttgttgtt
    12541 gtggtagaga atgctgggcg tgctgtcgca ggcaacgtaa atgtcgggcc agccatcgtt
    12601 gtcgtaatcg agcgtggaaa cacctagtcc gtagtgtccg ttggtctggt cgatatgcgc
    12661 cttgacggtc acgtcctcga aggtgccgtc gccccggttg tgatacagga tgttcttgtg
    12721 gtgtggcagt ccgcgcgggc cgcacatcac cggagcaccc ttccacatgc acgacggcct
    12781 gtctcccgga gctggggctg tggtcacgtc gaaatcaacg tagttcgcaa cgaaaagatc
    12841 gaggtggccg tcgcggtcgt agtccacaaa ggcgcagcct gcgccccact ccttgccatt
    12901 gccagcaact ccggctttct cgctcaccag tttgaagaca ccgtgatcgt tgtgatacag
    12961 ccggttcttg ccgtagtacg taacgtagag gtcttcccat ccatcgttgt cgtagtcgcc
    13021 gacgcagacg ccctgtcccc agcccgtcgc gtcgagcagc ccggcttttt ccgtgacgtc
    13081 agtgaacgtt ccgtcgtgat tgttgtggta gagatgattg gtcggtcccg acttcccccc
    13141 cttcatatct tcgagcgtgg tcccgttcac aaagaagatg tcgggccagc cgtcattgtc
    13201 ataatcgaag atggcgacgc ccgtcccggt ggtctcaatg atgtatttct tggtgtcgac
    13261 gccgccgaag acattcttca tcgtgaggcc cgccttctcg gcgatgtcca cgaagttcgc
    13321 gatcgattgg gacgaatttt tggttgactg tgccgtagca aacgtcgcca aggccatcag
    13381 aatcacggaa atgactggga ctcgcgccat cgcgcggaag aaataggcct gacagggaag
    13441 acgatacatc ctagctttcg atactaggga aaggcttctt gcagcgtcaa gcggacgcgc
    13501 cgccagttac gccgcagaga ttctgcagga ttcgcttttt ccgtgggtcg cttccatttc
    13561 gctgagccgc agcaccaggc atttggctgc tccgcccgcc ttaaggaact cgctcagcgg
    13621 cacctggatc acgcgaaacc ccagcgactc caaccggcca gacaattgcg gactgacctc
    13681 attcagcaga atggtcctac ccacattgat cgcgttgcag gcaaatcgaa gagcatcctg
    13741 caaggagacg gcgatgcgct gttcttcggg gtagcgggcc gcaattctag cttgggaaac
    13801 ggcatcgaag gcctcgggaa aatagaggac atagccgccg aagagcgggc agaaacacgt
    13861 gtcgaggtga tagaaacggg gatcgaccag ccgtagtgaa atgacttcga cgccccaggt
    13921 atcttccaga tacgggtggc tggcctctcg gctgcgcatg ccgtgtgcgg cccagagccg
    13981 tgagttgtcg gcttcaaata gggcgtcgcc ttcgccttca aagggcacag cccggggcag
    14041 ttcgcgaact gcaaagccgg aatcactaaa ccacttgcgg aaatgcggct cctccccctg
    14101 gcgctcggga tgaaggaacc ggctgagcgt caccactcgc ttgcgtacca agccggcgtt
    14161 ggcggtgaag accatgtcgg gagagccagc ctgcggttcc accaattcca cccgcgccag
    14221 accgaccagg gcggcacgca actgctccca ttgccgttcg gcattctgac gcgaggagca
    14281 atggacgttg ccttccatcc agggattaat gacgtagttc acgtcgtaga gggtcggggg
    14341 gcacatcaga aacattggac cgccagagac acgcgcgtcc gatgcatttc gcaggaaagc
    14401 ctcacccagg ctcatctttg cctcgctgag ggagagtctg actcagtacc tggagggcgt
    14461 caaatccaaa atttctatga gccttatgtg ttactgccgc aaaatgaacg ttgcgttaca
    14521 acagtggctt cgcgcgagag gttagctact tcttcaccgg atagtcgttg aacaacttgg
    14581 tgaccgtgct gttggcttcc tccatcagtt tgtcggggtt gttggggcta atcgcttccg
    14641 agcccatgcc gcgccacacc aatttcttgg ccttggcgtc aatcaagtca atgatcaact
    14701 ggccgggatg cacgagcacg gtcgaattcc cgtagtagcc gtcccagtac aaggagtttc
    14761 ccggcgacgt catgctggga tcgaacggcg gaatgccgcc ggtcgcgtta tagatggggt
    14821 tgttcgaggt catgctcacc tcggtatcga ccgcgccata gacctgcacg atgaggtcgg
    14881 gattcgcagg gactttcttc agtccgcgcg cgttcagttc gccttcgata gcgccgacaa
    14941 cgtccagggc gagcatggga tgcgcgaccg caccgattgg ggcccatccg tacgtcttgt
    15001 acttggaaaa gtcgaccgac ttgtcgtagt ggacgcggat tttctcggct tgcgcgccgg
    15061 cgcacaagac aatgaggact agcagaaacc gaatagcgca gaagcgcgca cgacgtttca
    15121 tcaaagacat tcgtatccct tcacgcataa acggataatc agaaagtagc ttgctttgcc
    15181 gcgatcaaac caacccggtg cgatcaaaca agtcgcgaac tgccgaggtc ccgatttcga
    15241 ccgaaaaaat gaagaacctg ttcaaggctc gaccaccgat taattaattc tatcgccgaa
    15301 atccgccgcc gccgccgccc ccgccaccgt aactccgtcc gccgccaccc caaccgc
//
LOCUS       DASRKK010000330         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_416115, whole genome shotgun sequence.
ACCESSION   DASRKK010000330 DASRKK010000000
VERSION     DASRKK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416115"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..1121)
                     /locus_tag="VFC15_07090"
     CDS             complement(450..1121)
                     /locus_tag="VFC15_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02453 family protein"
                     /protein_id="HZM09958.1"
                     /translation="MPDDATFSPDLFTLLRQLKRHNNREWFAKNKTRYHDVVVEPALQ
                     FINEFAPRLYKLSPFFLADARPTRGSLFRIYRDIRFSTDKRPFKTHVGIHFHHATGKD
                     AHAPVFYLHFEPDNCFAAAGIWHPDNPALTRVRAAIVREPEKWAAVRKKLELEGDSLA
                     RPPRGFDPGHRFIDDLKMKSYITSIPFTEQQVCGQKFMRDFVAACRKMSPLVEFTTKA
                     LGQKY"
     gene            1320..>2114
                     /locus_tag="VFC15_07095"
     CDS             1320..>2114
                     /locus_tag="VFC15_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HZM09959.1"
                     /translation="MKKLRIIGQIVCAVITLTSVTANACTRFIYETGNKTFVVGRSMD
                     WMEDPKTDLWAFPQGMKRDGGLGQGSMIWTSKYGSVIASFYNVATVEGMNSAGLVANT
                     LYLVETDYGDAKVAGKPLMSVGAWTQYVLDNFATVAEAVDALSQEPFAIVAPALPDGS
                     AAGGHLSLADASGDSAIFEYLNGKLVIHHSPKYTVMTNSPTFDQQLAIATYWKNIDGL
                     SFLPGTINSSDRFVRASWALNAAPKEKDNRLAIATSFSLIRSISVPL"
BASE COUNT          469 a          581 c          596 g          468 t
ORIGIN      
        1 atgaatgcca agcaattagt gcccatccac ggcgctgcat gggacgggga cacgactggg
       61 tttccctcca tcaggcgcct agccgatggt gagtcgatga cactttaaca gcactagggg
      121 cagcccggat ggacaggtgc agcccaaaag ccgcgcgtcc gccgcaaagc ggcttcgtta
      181 aagtcggcca gtgtttacga atgctttcgt ttcacatgga cgtaaaacgc ccatatcgtc
      241 gatgtgcggg cagccatggc cctgaagtcg taatgatcga agcggttgtg ctgatcaaag
      301 cagattagtc ggtttaaggc cctcgtgtcc cggaagacgc cctcggctca ggtgcattcg
      361 gttcggtagc taacagcatc gtgaagctca gcttgtgaaa atggccggtc cgacgcctgg
      421 gttcaaggga tcaagcatgt tgtcctgcat tagtactttt gccccagcgc cttcgtcgtg
      481 aactccacga gtggtgacat tttgcgacaa gcagcaacga agtcgcgcat gaatttctgt
      541 ccacatacct gctgctcagt gaatggaatc gaagttatat agctcttcat cttcaggtca
      601 tcgatgaagc gatgaccggg atcgaatcca cgaggcggcc ttgccaggct gtcaccctcc
      661 agctccagtt tctttcgaac tgctgcccac ttctcgggct cgcgtacgat tgccgcccgc
      721 actcgtgtca gagcgggatt gtcggggtgc cagattccag ccgcagcgaa acagttgtca
      781 ggctcaaagt gtaggtagaa caccggcgca tgcgcatcct tgccggtggc gtgatggaaa
      841 tgaattccaa cgtgggtctt aaagggacgc ttgtcggtgg agaatctgat atctcgataa
      901 atacgaaaca gggaaccgcg tgtgggtcgg gcgtcagcaa gaaagaaggg actcagcttg
      961 tacaggcgag gcgcgaattc attgatgaac tggagtgcgg gttcaaccac cacatcgtga
     1021 tatcgcgttt tattcttggc gaaccattcg cgattgttgt ggcgcttcag ttggcgcaat
     1081 aaggtgaaca gatcagggct gaaagtggca tcgtcaggca tcgagggctc cctctacatc
     1141 actaattctt cgccactggc ccgcatgcct gcaactcccg ccacaccagc agttcacagc
     1201 cagaacgtga cacaagtcac tgcgtgaccg aattgaggag tgctactgtc atgctgcata
     1261 ttttggggca gagaaatatg caaacttagg ctataaactc gcccgggaga ggtcgtcaaa
     1321 tgaagaagct tcgcatcatc ggccagatcg tctgcgccgt gatcaccctg acgagtgtta
     1381 ccgccaatgc ctgcacccgc ttcatttatg agacggggaa caagaccttc gttgtcggcc
     1441 gctcgatgga ttggatggag gaccccaaaa cagacctatg ggcttttccg caaggcatga
     1501 agcgtgatgg cggcctgggg cagggttcca tgatctggac gtcgaaatac ggttcggtga
     1561 tcgcatcctt ctataatgtc gctactgttg aaggcatgaa ttccgccggt ttggtcgcca
     1621 acacgctgta tctcgtcgaa acggattacg gggatgccaa ggttgccggc aagccgctga
     1681 tgtcggtggg cgcctggacg caatatgtgc tcgataactt cgccaccgtg gccgaggccg
     1741 tagacgcgct ctctcaggaa cccttcgcca ttgtcgcgcc ggcacttccc gatggcagtg
     1801 cggcaggtgg acatctttct ctagcggacg cctccggcga tagcgcgatc ttcgaatatc
     1861 tgaacggcaa actcgttatc caccatagcc cgaaatatac ggtgatgacg aactcgccga
     1921 ccttcgacca gcaactggcg atcgcgacct attggaagaa tatcgacggc ctctctttcc
     1981 tgccgggcac aatcaattcc tccgatcgct tcgtgcgcgc aagctgggct ctcaatgccg
     2041 cgccaaagga gaaggacaat cgcctggcga tcgccacatc gttttcgctg atccgtagca
     2101 tctcggtgcc gctc
//
LOCUS       DASRKK010000331         7239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1100528, whole genome shotgun sequence.
ACCESSION   DASRKK010000331 DASRKK010000000
VERSION     DASRKK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100528"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1088
                     /gene="coxB"
                     /locus_tag="VFC15_07100"
     CDS             105..1088
                     /gene="coxB"
                     /locus_tag="VFC15_07100"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZM09960.1"
                     /translation="MWKNLPLFPEQASSMASQVDGLYFLLVAVSAFFTLLIFALVFVF
                     AVKYRHSAHPKAEQIEGSLPLELTWTLIPLGISMIFFAWGALIYFQEARPPKGAMEVY
                     AVGKQWMWKFEHETGQREINTLHLPVNRDVKMIMSSQDTIHSFFVPAFRIKQDVLPGR
                     FTSTWFHTTKVGTYHLFCAEYCGTEHSGMIGEVVVMEPTAYQAWLANGGATGTMAANG
                     KELFTQLGCATCHRFDTQGRGPNLNGVFGKPVQLDDGRTVMADENYIRESILNPQAKV
                     VSGFKPIMPTFQGQVTEESLGALVSYVKSLAQPQQKAAGGTPGPAATSESN"
     gene            1114..2748
                     /gene="ctaD"
                     /locus_tag="VFC15_07105"
     CDS             1114..2748
                     /gene="ctaD"
                     /locus_tag="VFC15_07105"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZM09961.1"
                     /translation="MATAVQTTVETENYLNNGYGLKSWLLTRDHKRIAILYLISITAM
                     FFVGGFFAMLIRLELLTPAGDLVSSDTYNKLFTMHGISMIFFFLIPSVPAVLGNFLIP
                     LMVGAKDLAFPRINLLSWYLYIIGATFALVSMVMGGVDTGWTFYTPFSTTYSNTHVIG
                     TALGIFIAGFSSIFTGLNFIVTIHRMRAPGMTWTRLPLFCWSNYAASIIMVLGTPVVA
                     ITILLVALERLFHIGVFNPALGGDPLLFQHLFWFYSHPAVYIMILPGMGVISEIVTCF
                     SRKRVFGYEFVALASIAIAVLGFLVWAHHMFVAGISVYAALVFSLLSYLVAIPSAVKI
                     FNWTATLYKGSIRYDTPMLYAFGFMGLFTIGGLTGVFLASIGVDVHVTDTYFVVAHFH
                     YVMVGGMVLAYMGGLHFWWPKMTGRMYPEGWAKLSALLVFVGFNLTFFPQFILGYMGM
                     PRRYHAYPPEFQVLNVLSTAGASVLAVGYVLPMIYFVWSLRYGKLAGPNPWNAVGLEW
                     MTPSPPPTQNFDETPVVTWEAYDYEHQPDEEVPVGH"
     gene            2738..3421
                     /locus_tag="VFC15_07110"
     CDS             2738..3421
                     /locus_tag="VFC15_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HZM09962.1"
                     /translation="MATSDIEIAEHSLQHHFADMDQQKDASTFGMWLFLVTEIMFFGG
                     LFCAYLVYRSTYYQAFVEGSQTMNIWLGATNTAVLICSSLSMALGVRCAQIGKTKMMV
                     VLLLITIVFGFAFLGIKGVEYHEHWVNHQFPGPTFQFEGAEAAGVNPQNIQIYFSLYW
                     AMTGLHALHMVIGIGLVTWIVIAGWRGAYSPDYYSPVENVGLYWHFVDLVWIYLFPLL
                     YLISKKHGG"
     gene            3432..3746
                     /locus_tag="VFC15_07115"
     CDS             3432..3746
                     /locus_tag="VFC15_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZM09963.1"
                     /translation="MSVEEHRQHVIPTSIYVGIWAVLMVFTGVTVFASFIELGDWNIV
                     LALTIATIKATLVVLFFMHLYYSSKLTKVTMIAAIFFLFLLLALSMTDYLTRSWLTNP
                     GH"
     gene            complement(3829..4875)
                     /locus_tag="VFC15_07120"
     CDS             complement(3829..4875)
                     /locus_tag="VFC15_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM09964.1"
                     /translation="MRKKALIGGVLILLAVTSMAADQPSDSLSLSDMNAGGTTRNGSP
                     NQVLAGLSSTAGPRRQAQTLAESIFPDFTIRRTVPEVRFQFSVADERGQLVTDLSPSD
                     FRIFDNQSAVQQIRQFSRLDDLSLQLGILLDVSDSVQKTTTREKLAAQIFVQQVLRPQ
                     TDRAFLMAFARDVKLWQPSTGDATALRRSLELIQQAGYATNLYDGVFSACFGRFPQSD
                     GPGVVQRVVVLFSDGEDTGSLHGMPDVIALAQRNEIQIFALSIHARRKPTTGDEVLHR
                     LADETGGRFYVAASDKELLAIFTEMEQQMRRQYAVSFQPQQETPGFHALRVELNSPQN
                     LRVRARQGYYFQAP"
     gene            5109..5804
                     /locus_tag="VFC15_07125"
     CDS             5109..5804
                     /locus_tag="VFC15_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM09965.1"
                     /translation="MKQTIFAVEDDTDISRLVRHHLEAAGFGVRSFAAPAGVITEAEK
                     QSPALFLLDIMLPGGDGLELCRRIRQTPALSMVPVIFLTAKTTESDKVIGLELGADDY
                     IPKPFSPRELVARVRAVLRRFERPLAPAMVKAGDIEIDSGGMTLMVRGVLTQTTATEF
                     RLLEYLARHPGRVFTRDQLLDAVWRDTHYVTPRSVDVYVRRIREKIEANPDEPRYLKT
                     VRGAGYRFEVPKL"
     gene            5801..>7239
                     /locus_tag="VFC15_07130"
     CDS             5801..>7239
                     /locus_tag="VFC15_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZM09966.1"
                     /translation="MKSRIFLKLFAASLVLIAACTLTMNVLIQKAWEGMLRSEIESSL
                     REKTLLFASRVANAPRESLPAIAKQAARDSDERATVIDSNGNVLADSEADPSSMENHA
                     GRPEFASALQGQLGVATRASHTVGVDFLYEAAPFPGGAVRLAHPLSAIRDANRHVRKD
                     LLKSSAVAALIALVLAFIATQSIGRRLARITDFAERVAAGDLSARIEEESTDEIAHVA
                     SALDKTARKLEEGFRTVESSRQTLETLLNSMQEAVIAVAEDGRVLWANHRMERLLPSG
                     IRLGEPLVQSARDPEILRAVQTALDSRDITVARAAKIFSGRIFDVTAAPMPGGGVVAV
                     MHDQTEVERVEKTRRDFIANVSHELRTPLTSIQGYAETVLENEELSESTREFVEIIRK
                     NALRMSRLTEDLLVLARVESGERGLQLESATPQELLEEAVQTFQEIADGRGIELAVMN
                     TANSAVMVDRDAIHQVFGNLIDNAMKYGEG"
BASE COUNT         1434 a         1926 c         2299 g         1580 t
ORIGIN      
        1 ttgggaggag cggccacggt gctgctgctg ggcggattcc tgatcgtgat gtatcgacgt
       61 gaacctcatg attcccgaaa gggagcagga cgggcctgat agatatgtgg aagaacttgc
      121 cactgtttcc cgagcaagcc tcgtcgatgg cgagccaggt ggatggactt tacttcctcc
      181 tggtggcggt gtcggcgttc tttacgctgc tcattttcgc gctggtgttc gtgtttgcgg
      241 tgaagtaccg gcactcggcg cacccaaagg cggagcagat tgaaggctcg cttccgttgg
      301 aattgacgtg gacgctgatt ccgctgggca tttcgatgat cttcttcgcg tggggagcgt
      361 tgatctactt ccaggaagcg cgtccgccga agggcgcgat ggaagtgtat gccgtaggca
      421 aacagtggat gtggaagttc gagcacgaga ccggccagcg cgagatcaac acgctgcatt
      481 tgccggtgaa tcgcgacgtc aagatgatca tgagctcgca ggacacgatc cactccttct
      541 tcgtgccggc attccggatc aagcaggatg tgctgccggg ccgcttcacc tcgacgtggt
      601 tccataccac gaaggtcggt acctatcatc tgttctgcgc ggaatattgc gggaccgagc
      661 actcggggat gatcggtgaa gtggtagtga tggaaccgac tgcgtatcag gcgtggctgg
      721 ccaacggcgg ggctactgga acgatggcag cgaatggcaa ggaactgttc acgcagttgg
      781 gatgcgcgac ctgccatcgc ttcgacaccc agggccgagg tccgaacctg aatggagttt
      841 tcggcaagcc ggtacaactg gatgacggac gcacggtcat ggcggatgaa aactacattc
      901 gcgagtccat cctgaatcca caggccaagg tggtgtcggg attcaaaccc atcatgccaa
      961 cgttccaggg acaggtgacg gaagagagtt taggggcgct ggtgagttat gtgaaatcgc
     1021 tggcacaacc acagcagaaa gcggcgggtg gaactccggg gccggcggcg acgagcgaga
     1081 gcaattaggt ctttaaccct gaaggtgtgt gacatggcaa ctgctgtaca gacgacggtc
     1141 gagacggaaa actacctgaa taacggctac ggtctgaagt cgtggctgtt gacgagagac
     1201 cataagcgga tcgcgattct gtacctcatc tcgatcaccg cgatgttctt cgtgggcggg
     1261 ttctttgcca tgctgatccg gttggaactg ctcacgcccg ccggggacct ggtttcatcc
     1321 gatacatata acaagctgtt caccatgcac gggatctcga tgattttctt tttcctgatt
     1381 ccctcggtgc cggcggtttt gggcaacttt ctgatcccgc tcatggtcgg tgccaaagac
     1441 ctggcatttc ccagaatcaa tctgctgagc tggtacctgt acattatcgg tgcaacgttc
     1501 gcgctggttt cgatggttat gggcggagtg gatacggggt ggaccttcta cacgccgttc
     1561 agcaccacgt attcgaacac ccatgtgatt gggacagccc tgggcatatt tatcgcggga
     1621 ttttcgtcaa tcttcactgg gctgaacttt attgtcacga ttcatcgcat gcgcgcgccg
     1681 ggaatgacct ggacgcggct gccgctgttc tgctggtcga attatgcggc cagcatcatt
     1741 atggtgctcg gcacgccggt ggtggccatc acgatcctgc tggtggcgct ggagcgcttg
     1801 ttccacatcg gcgtgttcaa cccggcgctg ggtggggatc cgctgctgtt ccagcacctg
     1861 ttctggttct actcccaccc ggcggtgtac atcatgattt tgcctggcat gggagtgatc
     1921 agcgagattg tgacctgctt ctcgcgcaag cgggtgttcg ggtatgagtt cgtagcgctg
     1981 gcctcgattg cgattgcggt gctgggcttc ctggtgtggg cccaccacat gtttgtcgca
     2041 ggcatttcgg tttatgccgc actggtcttt tcgctgctga gctacctggt ggcgattcct
     2101 tcggcggtga aaatcttcaa ctggacggcc acgctgtaca agggttcgat ccggtatgac
     2161 acgccgatgc tttacgcgtt cgggttcatg gggctgttca cgatcggcgg gttgacggga
     2221 gttttcctgg cgtcgatagg agtggacgtc cacgtgaccg acacctactt tgtcgtggcg
     2281 cactttcact acgtgatggt gggaggcatg gtgctggcgt acatgggagg cttgcacttc
     2341 tggtggccga agatgacggg ccgtatgtat cccgaaggat gggccaagtt gtcggcgctg
     2401 ctggtttttg tgggattcaa tctgacgttc ttcccgcagt tcatccttgg gtatatgggc
     2461 atgccgcggc gctaccatgc gtatccgccg gaatttcagg tgctcaacgt tttgtcgacg
     2521 gcgggcgctt cggtgctggc cgtaggctat gtactgccca tgatttattt cgtgtggtcg
     2581 ctgcgttacg ggaagttggc gggaccgaat ccgtggaatg cggtgggcct ggagtggatg
     2641 acgccatcgc cgccgcccac tcagaatttt gacgagacac cggtcgtaac ctgggaagcc
     2701 tacgactacg agcatcagcc ggacgaggag gtccccgttg gccactagcg atatagaaat
     2761 cgctgaacac tcgctgcaac accacttcgc ggacatggac cagcagaagg atgcgtccac
     2821 gtttggcatg tggctgttcc tggtcacgga gatcatgttc tttggcggct tgttctgcgc
     2881 ttacctggtc tatcgctcga cgtattacca ggcgtttgtc gaaggtagcc agaccatgaa
     2941 catctggctg ggagcgacca acacggcggt gctgatctgc agttcgctgt cgatggcgct
     3001 gggagtgcgt tgcgcgcaga ttggtaagac caagatgatg gtcgtcctgt tgctgatcac
     3061 cattgtgttc ggtttcgcat tcctggggat caagggcgtg gaataccacg aacactgggt
     3121 caaccatcag ttcccgggac caactttcca gttcgagggc gcggaagctg ccggagtgaa
     3181 tccgcagaac attcagattt acttttcgct gtactgggcg atgaccggat tgcacgctct
     3241 gcacatggtg atcggaatcg ggctggtcac ttggatcgtg attgcgggat ggcgcggagc
     3301 ctacagcccg gactattaca gtccggtgga aaatgttgga ttgtactggc actttgtcga
     3361 tttggtgtgg atttatctct tcccgctgct ctacctgatc agcaagaaac acggcggtta
     3421 agcgaggacg catgtcagtt gaagaacatc gacaacacgt aatacctacc agcatttatg
     3481 ttgggatatg ggctgttctc atggtcttta ccggcgtgac cgtgtttgct tcgttcatcg
     3541 aactgggcga ttggaacatc gtgctggcgc tgaccatcgc taccatcaag gccacgctgg
     3601 tggtgctgtt cttcatgcac ctgtactaca gctcgaagct gacaaaggtt acgatgattg
     3661 cggccatctt cttcctgttc ctgttgctgg cgctgagtat gaccgactac ctgacgcgca
     3721 gctggctgac caatccggga cattgagtga agggtgtggc gaaagcgctc cgcatagtgc
     3781 ggggcgtttt tatttgcgag agagagttcc acactgtggt agtaagaact acggcgcttg
     3841 gaagtaataa ccctggcggg cgcggacacg cagattctgg gggctgttca gttcgacgcg
     3901 cagggcatga aagccgggtg tttcctgctg tggctggaag gagacagcgt attgcctacg
     3961 catttgctgc tccatctcgg tgaagatcgc gagtaactct ttgtcggagg cggccacgta
     4021 gaaacgccca ccagtctcgt ccgccaaccg atgcaacacc tcgtcgcccg tcgtgggttt
     4081 gcgcctggcg tggatcgaga gggcaaatat ctggatttcg ttgcgctgcg ccagggcgat
     4141 gacatccggc attccgtgca ggctaccggt gtcttcgccg tcgctgaata gcacgacgac
     4201 gcgctgcacc acacccggcc cgtcagattg cggaaaccga ccaaaacagg cggagaacac
     4261 gccgtcgtac aaattggtcg catagcccgc ttgttgaatt aactcgagag accggcgcaa
     4321 cgcagttgcg tctccggtcg acggctgcca tagcttcaca tccctggcga acgccatcag
     4381 gaaggcgcga tcggtctgtg gccgcaggac ctgctgaacg aagatttgag ccgccagctt
     4441 ttcgcgagtg gtagttttct ggaccgagtc gctcacgtcg agcagtatgc cgagctgcaa
     4501 cgaaaggtcg tcgaggcgcg agaactggcg gatctgctgc acggcggatt ggttgtcgaa
     4561 aatacggaaa tcgctggggg agagatcggt cacgagttgc ccacgctcgt cggcaacgct
     4621 gaactggaac cgcacctcgg gaacggtgcg gcggatggtg aagtcaggaa aaatggactc
     4681 ggctagtgtc tgggcttggc ggcggggacc ggcagttgat gacagacctg cgaggacctg
     4741 gtttggggaa ccgttgcggg tcgtgccgcc cgcgttcatg tcgctcagac tgagggaatc
     4801 cgaaggctga tcggctgcca tcgaggttac cgccaggaga atcagaactc cgccgatgag
     4861 agccttcttc cgcatagggg aattatcggc gggtcgcggc cttgcttgag tcggcagcag
     4921 agccgtgcaa tcccaaaatc actcgatata gccggatgga cacaggctcc cggaaattaa
     4981 ggagtgatca atgggttagt aaatcgttca tcggactgta atcaccctgt aacaagctgg
     5041 gatgtaacgt aattgctgtg atgctaggac atgagagctc tgccaacacc gtcggctaca
     5101 atacagccgt gaaacaaacc atttttgccg tggaagatga cacggacata tcccggctgg
     5161 tccgccacca tctggaggct gccggcttcg gcgttcgctc cttcgcggct cctgccgggg
     5221 taatcaccga agcggaaaag cagtccccag cgctgtttct gctggacatc atgctgccgg
     5281 ggggcgacgg cctcgagtta tgccgccgca tccgtcagac gccggcacta tcgatggttc
     5341 cggtgatttt tctcaccgca aagaccaccg agtcggacaa agtgatcggg ttggaactgg
     5401 gggctgacga ttacattccc aagcctttca gtccacgcga actggtggca cgagtgcgag
     5461 cggtgctgcg ccgcttcgaa cgtccgctgg cgcccgccat ggtgaaagcc ggcgacatcg
     5521 agatcgacag cgggggcatg accttgatgg tccggggggt gttgactcag accacggcca
     5581 cggaatttcg actgctggaa tatcttgccc ggcatcccgg ccgcgtgttt acccgcgacc
     5641 aactgctcga tgccgtctgg cgggacaccc attacgtgac gccacgctcg gtggacgtgt
     5701 acgtgcgccg cattcgcgag aagatcgagg cgaatccgga cgagccacgc tacttgaaga
     5761 cggtgcgcgg agcaggctat cgcttcgagg tcccgaaact gtgaaaagcc gcatttttct
     5821 taagctgttt gccgcctcgc tggtactgat cgcggcatgc acgctcacga tgaatgtgct
     5881 gatccagaaa gcctgggaag ggatgctgcg cagcgaaatt gaaagttcgc tgcgcgagaa
     5941 aaccctgctg tttgcttcgc gggtcgcgaa cgcaccccgc gaatcgcttc cggcgattgc
     6001 aaaacaagcg gcacgcgatt ctgacgagcg cgcgaccgtc attgattcca acggaaatgt
     6061 gctagctgat tccgaggccg acccttcctc gatggagaat cacgcagggc gtccggagtt
     6121 tgcctccgcc ttgcagggtc aattgggcgt agctaccagg gcatcccaca ctgtgggagt
     6181 cgatttcctc tacgaggctg caccctttcc tgggggcgcg gtccggctgg cacaccccct
     6241 gtcagcgatt cgcgatgcga accggcatgt acgcaaggac ctgctgaaga gttcggcggt
     6301 tgcggcctta atcgcactgg tgctggcgtt catcgcaaca cagtcgattg gacggaggct
     6361 ggcgcgcatt accgattttg cagagcgggt ggcggcgggc gatctctcgg cccgcatcga
     6421 ggaagaatcg acagatgaaa tcgcgcatgt cgcatccgca ctggacaaga cggcgcgcaa
     6481 gctggaggaa ggattccgaa cggtggagtc gagccgccag accctggaga ctctgctgaa
     6541 ctcgatgcag gaagcggtga tcgcggtggc ggaagatggg cgcgtgctgt gggcgaatca
     6601 ccgcatggaa cgcctgctgc ccagcggcat ccggctgggt gagccgttag tgcaatcggc
     6661 gcgcgatccg gagatcctgc gggccgtgca gacggccttg gacagccggg acatcacagt
     6721 ggcgcgagcc gccaagattt tctcgggccg catcttcgat gtgaccgctg cgcccatgcc
     6781 gggcggaggc gtggtcgccg tgatgcacga tcagacagaa gttgagcggg tggagaagac
     6841 gcggcgcgat tttatcgcca atgtgtcgca cgagctgcgc acgccgctga cctcgatcca
     6901 gggatacgcc gagactgtgc tggagaatga ggaactgagc gaaagcacgc gggagttcgt
     6961 ggagatcatc cgcaagaacg cattgcgaat gtcgcggctg accgaagacc tgctggtgct
     7021 ggcgagagtg gagtcgggcg aacgcggctt gcaattggaa tcggcgacgc cccaggaatt
     7081 gctggaagaa gcggtgcaaa ccttccagga gattgccgac ggccggggga tcgagttagc
     7141 ggtaatgaat accgccaact ccgccgtgat ggttgaccgc gatgccattc accaggtgtt
     7201 tggcaacctg atcgacaatg ccatgaagta cggagaagg
//
LOCUS       DASRKK010000332         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_611061, whole genome shotgun sequence.
ACCESSION   DASRKK010000332 DASRKK010000000
VERSION     DASRKK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611061"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFC15_07135"
     CDS             complement(<1..1154)
                     /locus_tag="VFC15_07135"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZM09967.1"
                     /translation="MDHRLILVTVLIRLGVAAAISSVLVRARRFRVLLFREERTLSEK
                     IEIVFIVGTPIALGVLVRGWVHNFQAADIAFEGAILMGVIGGRLAGTAGGILVSLPAL
                     FLGEWLTLPMNVLAGFLAGVLRHLGPDREAIWSFSPLFDLSIYHWIRRSIRKSFIDWQ
                     TSFFFLILGLEFIRIQLHRAFPKRIFALDANTWPINLAIYAGTVMAVAIALKVLNNVR
                     IEMKLEQQERLLLQARMEALQSQINPHFLFNTLNSVASLVRRDPDSAREMIVKLGNIL
                     RRLLRKGDSFVPLREELEFLDDYLDIEVARFGRDKLKVVKELEPASLDLLVPSMILQP
                     LVENAVKHGFSSKVDGGSIYIRSRCADHRVVIEVEDDGVGMSVSGASPSSG"
     gene            complement(1303..2529)
                     /locus_tag="VFC15_07140"
     CDS             complement(1303..2529)
                     /locus_tag="VFC15_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZM09968.1"
                     /translation="MTERLYYTDSFLTEFEARVVAVSREAGNIAVTLDRSAFYPTSGG
                     QVFDTGWLELLGVGDLQRIRVKEVAENEQTGDVLHMVEAGADALQPGALVRGTIDAER
                     RLDHMQQHSGQHVLSAAFEKLYGFPTVSFHMGDESCTIDLDGGAIVAKQLESAEKLAN
                     EVITEDRPVEIRFATPDEARAMGVRKIPPTERDQLRLIEVRDFDLNACGGTHVRSTGQ
                     IGAILLRKTEKVKQGVRVEFVCGLRAVRTARHDFETLTEAAAVFSTHIWEVPQQVRKS
                     LDEIKSAQKAQHRLLEEVADLQAEHMLQVAADRGAYKLVVQFFPDRDLGYLKMLAQKL
                     TRNGKAVALLSCGGSQPSLVFGQTPGLANDMGALMKQTVQQLGTRGGGNRDMAQGAAP
                     DSTSAEQALLQAASAL"
     gene            complement(2607..2906)
                     /locus_tag="VFC15_07145"
     CDS             complement(2607..2906)
                     /locus_tag="VFC15_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZM09969.1"
                     /translation="MIFNPAHPGEVLRDYLGGISVAEAATRLGVTRAHLSRILNGHAG
                     VSASMSLRLSATLNTSPEFWLKMQMQYDLWQAQKAKRPKIRLFPHVPKAKDLALA"
     gene            3003..3632
                     /locus_tag="VFC15_07150"
     CDS             3003..3632
                     /locus_tag="VFC15_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HZM09970.1"
                     /translation="MKTILRVTFFVVLGGLCWMPLAAQDAAMSAKPKAAKPKAQMAMP
                     MPKPAPEMTKMIKMMAGSWTVTEKHDPNPMMPNGGTGKGTAVLTPGPGNMSLMEKYHT
                     SGAMGSSFNGFGTFWWDPKAQVYRGVWCDNMSPNGCDDSGTTKWDGDKLVGMMESEMD
                     GKKMMTRFTYSDFKPDSFVMTMEMGPDASKMQKAMTITYTKAGMAEKKM"
     gene            complement(3882..4223)
                     /gene="secG"
                     /locus_tag="VFC15_07155"
     CDS             complement(3882..4223)
                     /gene="secG"
                     /locus_tag="VFC15_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZM09971.1"
                     /translation="MVVLITVIHIVVCLFLIIVVLLQSGQSADIAAAFGGMGSQTAFG
                     TRTAATVLSRATTWATIMFMVTSITLSVFATRHANRSGSVLEGSRPAPTRSAPMQQKP
                     APAPQPTPPSR"
     gene            complement(4809..>5055)
                     /locus_tag="VFC15_07160"
     CDS             complement(4809..>5055)
                     /locus_tag="VFC15_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM09972.1"
                     /translation="ERVRAGLRNARAKGKRLGRPPSGVNPSQISALRASGSSWRAISR
                     ELGVGVATLYRAVPVASPACEGRTLSSEPAVIASICS"
BASE COUNT         1089 a         1504 c         1486 g          976 t
ORIGIN      
        1 cctgacgaag ggctggcgcc cgagacggac atcccgactc catcgtcttc cacctcgatg
       61 accacgcggt ggtcggcgca gcggctgcga atgtagatgc ttcccccgtc aaccttggaa
      121 gaaaagccat gcttgaccgc attctccacc agcggttgca ggatcatact gggcacgaga
      181 aggtcgagcg aggcaggttc gagttccttg accaccttca gcttgtcacg cccaaagcgc
      241 gccacttcga tatcgagata atcatccaga aactccaact cctcgcgcag ggggacgaaa
      301 gagtctccct tgcgcagcaa ccgccgcaga atgttgccca gcttcacgat catctcgcgg
      361 gcggaatcgg gatcgcggcg caccagcgaa gccaccgaat tcagcgtgtt gaacaggaag
      421 tggggattga tctggctttg cagcgcttcc atgcgcgcct gcaacagcag gcgctcctgc
      481 tgctccagtt tcatctcgat gcgtacgttg ttcagcacct tgagcgcgat ggcgactgcc
      541 atgacggtgc ccgcgtagat cgccaggttg atcggccagg tattcgcatc gagcgcaaaa
      601 atgcgcttgg gaaaggcgcg atgcagttgt atacggatga actccagacc caggatgagg
      661 aagaagaacg aggtctgcca atcgatgaaa gacttgcgga tgctgcgtcg aatccagtga
      721 tagatgctga ggtcgaaaag cggcgaaaac gaccagatcg cctcacgatc ggggccaaga
      781 tggcgcagca cgccagccag aaatcccgcc aggacgttca tcggcaacgt aagccactcg
      841 cccagaaaca gcgccggcag cgaaaccagg atgcctcccg cggtgccagc cagacgaccg
      901 ccgattacgc ccatcaggat ggcgccctcg aaagcgatgt cagcagcctg gaagttatgc
      961 acccaaccgc gcaccagcac accgagcgcg atcggagtgc cgacgatgaa gacaatctcg
     1021 atcttctcgc tgagcgtgcg ttcctcgcga aacagcagaa cgcgaaagcg gcgcgcacgt
     1081 accagaacgc tgctgattgc cgcggcgacg ccgaggcgga tcagcacggt aacgagaatg
     1141 aggcggtggt ccattcagtg gttagtggtt agtgaatagt ggttagaaaa aagggccggc
     1201 tggttgtctg aaaaacccgt ttagaatttc agcgctcagt caaagcagcc gaacgagata
     1261 tttgcaactg agaaccagcc actaaccact aggcactaac cactatagcg cgctggcggc
     1321 ctgaagcaga gcctgttccg cgctggtgct gtcgggcgcg gcgccctgcg ccatgtcacg
     1381 attgccgccg ccgcgcgtgc cgagctgctg cacagtttgc ttcattaaag cgcccatgtc
     1441 attcgcgagc cccggcgttt gcccgaacac cagcgacggc tgcgaaccgc cgcaactcag
     1501 cagcgcgacc gctttaccgt tccgggtgag tttctgtgcc agcatcttga ggtagccgag
     1561 atcgcgatcg ggaaagaact gcactaccag tttgtacgcg ccacggtctg ccgccacttg
     1621 cagcatgtgt tcggcttgaa ggtccgcgac ctcttccagc agccgatgct gcgctttttg
     1681 agctgacttg atctcgtcga gagacttgcg cacctgctgg ggaacttccc agatgtgagt
     1741 cgaaaacacg gcggcagcct cggtgagagt ctcaaaatcg tggcgcgcag tgcgaacagc
     1801 gcgcaggccg cacacgaact cgacgcgaac gccctgctta accttctcag tcttgcgcag
     1861 caggatcgcg ccaatctgtc cggtactgcg cacgtgggta ccgccgcagg cgttcaaatc
     1921 gaagtcgcgc acctcgatca ggcggagctg gtcgcgttct gtaggaggta tcttgcgcac
     1981 gcccatggcg cgggcctcgt ctggagtggc gaagcgaatc tcgaccggac gatcttcggt
     2041 gatcacttcg ttggccagct tctcggccga ttccaattgc ttagcgacga tcgcgccgcc
     2101 gtcgagatcg atggtgcagc tctcgtcgcc catgtggaac gaaacggtag gaaatccgta
     2161 aagcttttcg aaagcggcgg agagcacgtg ctgaccggag tgctgctgca tgtgatcgag
     2221 acggcgttca gcgtcgatgg tgccgcgcac gagtgcgcca ggttgcagag cgtctgcgcc
     2281 agcctcaacc atatgtagca cgtctccggt ctgctcgttt tcggcgacct ccttcacccg
     2341 aatccgctgg aggtcaccga cgcctagcag ttccagccag ccggtgtcga agacctgtcc
     2401 gccactggtg ggatagaacg ccgagcggtc gagagtgacc gcgatgtttc cggcttcgcg
     2461 actgacggcg accacgcgag cttcgaattc agtgagaaag gagtcggtgt agtagaggcg
     2521 ctcggtcaag gtcattactc cggttagcgc ggcatctaga gttccagggt agcgccctcg
     2581 aatgacgatc tgtccccgat ccccgcttag gccagcgcca aatctttggc ctttggcacg
     2641 tgcgggaaca gacggatttt cggccgcttc gctttctgcg cttgccatag gtcatactgc
     2701 atctgcatct taagccagaa ctcaggcgag gtgttgagcg tcgccgacag cctcagcgac
     2761 atcgaggccg acactccggc atgcccgttc agaattcggg acaggtgtgc gcgggtcacg
     2821 cccagacgcg tggccgcctc cgcaacgctt atcccgccca agtagtccct caatacctct
     2881 cccggatgcg ctggattgaa aatcatggcg ttcctcctag ggtgtgcctc tgggctgttg
     2941 cgaaactccc catatcgtat aagattcttg ccgcacgatt ctacttccac aaaggagggg
     3001 ctatgaagac cattctacgg gtaacgtttt ttgtggtact gggcggactt tgctggatgc
     3061 cgctggctgc gcaggacgcg gccatgtcgg caaagcccaa ggcggccaaa ccgaaagcgc
     3121 agatggccat gcccatgccc aagcccgcgc cggaaatgac gaagatgatc aagatgatgg
     3181 cggggagctg gacggtgacc gagaagcacg atcccaatcc gatgatgccc aacggcggca
     3241 cgggcaaagg cacggctgta ctgacgcccg ggccgggcaa catgtcgttg atggagaagt
     3301 accacaccag cggagccatg ggcagcagct tcaacggatt cggaacgttc tggtgggacc
     3361 ccaaggcgca agtgtaccgg ggcgtttggt gcgataacat gtcgccgaat ggctgcgatg
     3421 atagcggcac caccaagtgg gacggagaca agctggttgg catgatggag agcgagatgg
     3481 acgggaagaa aatgatgacc cggttcacct attccgactt caagcctgac tcgttcgtga
     3541 tgaccatgga aatgggcccg gatgccagca aaatgcagaa ggcgatgacc atcacgtata
     3601 ccaaagccgg catggcagag aagaagatgt agcaggccca cgcaaaggga attcaaggcc
     3661 cgcagtcctg cgggctttga acccaactga gggtacttct agggaacctc gaaatcaaca
     3721 ttctgtatct tctgtatctc cggggctgaa gccaagaatc gcgggctttc taacgcgggc
     3781 ctgaaggccc gcactacccg agccacagcc actctggaat cagctcagac gccggcctga
     3841 aggccggcac tacccgaaca tcgaacttgt tcggagtttt cttagcggct cggcggcgtc
     3901 ggctggggtg cgggcgcagg tttctgctgc atcggggccg acctcgtggg cgccggtcgc
     3961 gagccttcca ataccgatcc gctacggttc gcatgacggg tggcgaacac cgaaagcgtg
     4021 atggacgtga ccatgaacat gatagtcgcc caggtggtgg ctctcgagag cacggttgcg
     4081 gcagtgcgag ttccgaaggc agtctggctg cccatgcctc caaaggcggc ggcaatgtca
     4141 gcactctgcc cgctttgcag cagcaccaca atgatgagga acagacaaac gacgatgtga
     4201 atgacggtaa tcaggacaac catggagctc tcaatcctcg catccggcgg ccgcgcaagt
     4261 tacgcgaaac tgcgccggca tcggcaatat cgtaactctt tacgtcgctc ataacttggg
     4321 tgaggcatgg tgcggaaggg gggacttgaa ccgcgccgca tattgacccc acgccgtttc
     4381 gcttattggt gatggtctca ttgtacagaa gttccacctg ttacaccgtt gccttgcaaa
     4441 tccgtacaaa ttggcggatg gtatctgcca tccaattccg ccatgtgcgc atgtacccga
     4501 ttgtatcatt tcgacctcgt attcaatttt cgcttttacc ccatctaata tcgcgtctgt
     4561 ggtattgccg aacctcagct ttatcttcgt tccgttccca tatggctgta cgtcatccaa
     4621 agaaacggta atagctccga gcgacgcatg attctctcca gcctgccctc aaaagcaaag
     4681 ccgcctttcg gcggctcgcg ccccctctcc accaggggga tatcaacatt cttgttatac
     4741 agccttccca ggtcattttc aagggttccg acaattctcg tcatgcactt ccagatgacg
     4801 aagcgcgcct acgagcagat gcttgcgatt acggccggct cggaactgag ggtgcgcccc
     4861 tcgcacgccg gagaggccac aggaaccgcc cggtaaagcg tagccactcc aacccccaac
     4921 tcccgcgaga tcgcccgcca ggatgaaccg gaagcgcgca gagcggaaat ctgtgaaggg
     4981 ttcacgccgg agggcggtcg tccgagtcgc ttccccttgg ctctggcgtt ccgcagtccg
     5041 gcccttactc gttcc
//
LOCUS       DASRKK010000333         8062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_25695, whole genome shotgun sequence.
ACCESSION   DASRKK010000333 DASRKK010000000
VERSION     DASRKK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25695"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFC15_07165"
     CDS             <1..249
                     /locus_tag="VFC15_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09973.1"
                     /translation="PSPPIPLFTLRCAPHDTQRKTRGRVDRYSFLVRIFHSLLHAGLS
                     RRTAMAISRQQSGKSHVSDVKEQGRTSDSDASRREAQK"
     gene            355..684
                     /locus_tag="VFC15_07170"
     CDS             355..684
                     /locus_tag="VFC15_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZM09974.1"
                     /translation="MNGVPELLILRLLQEEEMYGYEIVQAIRSRTDAVIAVGEGVVYP
                     VLHGLERDGALRSRRKTVNGRSRVYYSVTSSGSRRFADLSNTWVNLADAIQKMLIGGH
                     YAEEGIR"
     gene            662..1381
                     /locus_tag="VFC15_07175"
     CDS             662..1381
                     /locus_tag="VFC15_07175"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HZM09975.1"
                     /translation="MPKKAFDKLRERLLVAGIAPRHVRRYLSELSDHFEDLTAEAERE
                     GRSEQDAESEAISRLGNTDQLAEAMLGQRQFKSWCARVPWAMFGIAPLLLLAGGYFLA
                     CLYLWWGWTVFKPGADTPFGSPSGPMYGLANFYFQAGKFYYISAPILAGWTIAFAAAR
                     QRLRSPWPTLAVVLIAWMGATAQIQASRSAVPRGLGHISMTFFVFGPSPQTISSELLS
                     ALIIFSLAILPYFVWRLRSFS"
     gene            1415..2659
                     /locus_tag="VFC15_07180"
     CDS             1415..2659
                     /locus_tag="VFC15_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09976.1"
                     /translation="MNASTKTRRLVRRFFKCVALLAIFGVFAVTILLLSLWIEHRTDV
                     TLPNPTGPFAVGRTIYDWTDTKTEDPLAPMPATQRELLVWIWYPAEAQQSAAGTDYLP
                     AELRTAVEHDRGPFIRFLTRDLTKVYAHSWPNAAVSTQQRSYPVVIMRAGASLEVWNF
                     TTLAEDLASHGYVVVGFDAPYRSRVVVFPDGRVIKRLPQNDPERCLERAGQEQDRCAD
                     KVQNAWTSDTAFVLDRLTALNATDPSGRLTGRLDMSRIGIFGHSFGGATAAQFCHDDA
                     RCKVGVDVDGALHGSVIQAGIDRPFMFLLSDHSREGDPEATQILAKMRSVYARLPPNG
                     RQFLEIRGASHFLFADDGVLKSRIMLRILRTLGIIRIDGRRQLAITAYCLRSFFDEHL
                     KGANATAPKIPSPLYRELQVLE"
     gene            3073..4116
                     /locus_tag="VFC15_07185"
     CDS             3073..4116
                     /locus_tag="VFC15_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09977.1"
                     /translation="MVEFNGIVSQSLAKNGQPVELLVQMSRGVRVVNLGVPPDPKVDS
                     KGDVENYGVTEINNCPSAVDPWYQVFGTFNPLWNVDPPESWVDTVDQVAVFQSILITV
                     VGVETGELVTFNQAIDGGLSVVTANAAGVAVIPALLAVRSVNENAALTRANRTALGNV
                     GTAGAIFQRVAMLQTPGAISHQLSGDSFSATITTTFANGATQVMSVSDLGVVQLSAAQ
                     VGASASAQAAIRPRSPAADASASPSATVDPQWPIQIPGLVKVLPVPGLQNAPVAIAEL
                     SDGTNLVLSREPDGCVRMAGMVPRWLDMPAVSGRWAISSSTGNRVAVFAVRPFGSPKC
                     CCGCHTAEQAARR"
     gene            4149..4346
                     /locus_tag="VFC15_07190"
     CDS             4149..4346
                     /locus_tag="VFC15_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09978.1"
                     /translation="MFNATVVPQGGLDYLTLWPDGEPKPLVSTLNALDGSITNNMAIV
                     PSTNGKVDAYASGITQLILVQ"
     gene            4540..5787
                     /locus_tag="VFC15_07195"
     CDS             4540..5787
                     /locus_tag="VFC15_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM09979.1"
                     /translation="MQNPESESGFLFRLPFAAMAIMFALAVAATQSAQAQTFTDLHSF
                     TNHGDGNSPFAGPTMDRAGNIYGVTLYGGAGHGTAFKLSKRAGNWILDTLHTFTGGSD
                     GDSPFGNVTLGPDGNVYGTTIFGGGGCTSGGCGTVFKLSPPPRICTHTQCPWTETVIY
                     RFSASDTGLHNPWGGVTFDSAGNIYGTTSAGGTGDCSGQGCGAVYKLTPSGGAWTASV
                     IYNFTGFEDGEFPMATMTIDPAGNLYGTTYYGNSVFELVRSGSGWTENTLYTFQDGAD
                     GGNPQAGVIFDSAGNLYGATTTVDGSPAGGVVYELTHSPGGWTYSVLYTYNHSGGLFG
                     NVAIDSAGNIYGTAVTGGIYQGGSVFKMTNSGGVWSLTDLHDFGGIGWPEGNVLLDAS
                     GNLYGTAIGGGQYGWGSVWEIQP"
     gene            6368..6733
                     /locus_tag="VFC15_07200"
     CDS             6368..6733
                     /locus_tag="VFC15_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09980.1"
                     /translation="MMVEPVVQLAPGVDLQTAIATLIKVAENVEIVRWIELPASVLLF
                     LLVPGDPDSGAVYVLDRNKGTWYAVDFEDEQFGGYSASQLEMLLKECNFLDLIERPGL
                     WRSGLRWVVMPGMRPETAV"
     gene            7007..7822
                     /locus_tag="VFC15_07205"
     CDS             7007..7822
                     /locus_tag="VFC15_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09981.1"
                     /translation="MLTGETIRQHLEGKITVGLYAINPSTQRCKWVAIDADYKNSMED
                     LLRLQYHLTQDKLEPALEMSKHGGHLWIFLATPLLAKECRIYIHDLALRLGVPVKGSG
                     LAEGIEVFPKHDAIGAGEFGNAIRGPLGIHRGANRRFWIYGADYTLEDQIAYLNRLRK
                     VTEAELRRFIAGKERPEPDFSPRPERVTSTLRKTGRGQSEFRILEHVGTVRKVGRNYV
                     ARCPSCADCGHDRSGDNLAILIEDPRFYKCWAGCTKEMIRAALGCPIRVRHSA"
     gene            7844..>8062
                     /locus_tag="VFC15_07210"
     CDS             7844..>8062
                     /locus_tag="VFC15_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09982.1"
                     /translation="MNQKRFSDALGLRLTRQALRVLKERGIFAHSPVSLEHQHLAKRY
                     VVRGLESGGAAGELGRYVTFTQEDGQPIE"
BASE COUNT         1713 a         2276 c         2385 g         1688 t
ORIGIN      
        1 cccagcccac ctatacccct gtttacgctt cgctgtgcac ctcacgatac ccaacgcaaa
       61 actcggggcc gagtggatcg ctactccttt ctcgtaagaa ttttccattc tctgcttcat
      121 gccggtttat cccggcgcac cgcgatggcg atttcacgac agcaatcggg taagagccac
      181 gtatccgatg taaaagaaca aggccgcacc agcgattccg acgcctcccg ccgtgaagcg
      241 cagaagtgaa acagattccc gtaacatgtc cgatgggcgt ggatattact ataccttgaa
      301 ccgctaggta taatcccctc acttatgaca gcgcgcgaaa cgaacccgaa tttcatgaac
      361 ggggtaccag aactcctgat tctgagactg ctccaggaag aggaaatgta cggctacgaa
      421 atcgttcaag cgatccggag ccgtacggac gcagtaatcg ctgtcggcga aggcgttgtc
      481 tatccggtgc tccacggttt ggaacgagac ggtgcgctca ggtcgcgacg caagaccgtc
      541 aacgggcgca gccgtgtcta ttactcggtg acgtccagcg gatcgcgacg ctttgctgac
      601 ctgtcgaata cctgggtcaa tctagcggat gcaatccaaa agatgctgat aggagggcac
      661 tatgccgaag aaggcattcg ataagcttcg ggaacgcttg ctggtggccg gcattgcgcc
      721 ccggcatgta cggcgctatc tatccgaact ttctgatcat ttcgaggact tgacggcgga
      781 agcagaacgc gagggacgca gcgagcagga tgcagagtcg gaggcaattt cgagacttgg
      841 caacaccgat cagctggccg aggcgatgct cggtcaacgt cagttcaagt cctggtgtgc
      901 ccgagttcca tgggcgatgt tcggtattgc tccgttgctc ttgctggcgg gcggctattt
      961 cctggcgtgc ctgtatttgt ggtggggttg gaccgtcttc aagccgggag cggatacacc
     1021 gttcggcagt ccctcgggcc cgatgtacgg gttggcaaat ttttattttc aggccggcaa
     1081 gttttactac attagcgcgc cgattcttgc gggctggacc attgcattcg ccgccgcccg
     1141 tcaaagattg agatccccgt ggccgacgct cgctgtggtc ctgatcgcat ggatgggagc
     1201 gaccgcccaa attcaggcga gtcggagcgc cgttccccgc ggactcgggc atatcagcat
     1261 gaccttcttc gtcttcgggc cttctccgca gacgatttcc agcgagttgc tctcggcact
     1321 catcatattc agcctcgcca tcttgccgta tttcgtttgg cgattacgaa gtttcagcta
     1381 gtcatctgta gtcactccgt gtgcgaggat tctgatgaac gcatcgacta agacgcggcg
     1441 actagtacgc cggtttttca aatgcgtggc gctcttagcg atctttggcg tcttcgctgt
     1501 tacgatcttg ctcctgtcac tttggatcga acacaggacc gatgtcacac taccaaatcc
     1561 caccggccca tttgcggttg gccgcacgat ctacgattgg acggacacga aaacagaaga
     1621 tccgctggcg cccatgcccg caacccagcg cgaactcctt gtctggatct ggtaccccgc
     1681 agaggcccaa caatcagccg ccggcactga ctacctgcct gccgaattgc gaacggcagt
     1741 cgaacacgat cgcggcccct tcatcaggtt cctgacgcga gatctgacga aggtgtatgc
     1801 gcacagctgg cccaatgccg cggtttcgac gcagcagcgg tcctatcccg tggtgatcat
     1861 gcgagcggga gcttctctgg aggtatggaa tttcacgacg ctcgcggaag atctggccag
     1921 ccacggctat gtggtcgtcg gcttcgacgc accctatcgc agccgggtgg tcgtctttcc
     1981 tgatggccga gtgataaaac ggctgccgca gaacgatccg gaacgctgcc tggaaagggc
     2041 gggccaggag caggaccgct gtgcggacaa ggtgcagaat gcgtggacct cagatacggc
     2101 cttcgtgctt gatcggttga ctgctctaaa tgctactgac ccgtccggca ggcttaccgg
     2161 ccggctagac atgtcacgca tagggatatt cggccactcc tttggaggcg ctacggccgc
     2221 acagttttgc catgacgatg ctcgttgcaa agttggcgtt gatgtggacg gcgctttgca
     2281 cggcagtgtc attcaagcgg gcatcgaccg tccctttatg ttcctcttga gcgaccatag
     2341 ccgcgagggc gatccggaag ccacgcagat cctggccaag atgcgatcgg tctatgcgcg
     2401 cctcccgccg aacggaagac aattcctgga gattcggggt gcgagccatt ttctattcgc
     2461 cgacgatggc gtcttaaaaa gcaggattat gttgcgaatt cttcgtaccc tgggaatcat
     2521 tcgaatcgat ggtcggcgcc aattagctat caccgcgtac tgtctccgca gcttcttcga
     2581 cgagcatctc aaaggggcaa acgccacagc gccgaaaatt ccatctccgc tctaccgcga
     2641 attacaagtc ctcgaataaa gcacgccgat gcgtcacggc gcacaggaag ttatcgattc
     2701 atcgatagct gttgcgaaca gcccaactcg taacctattt gaacaaggaa ataatgcttc
     2761 gcgcagcatt cgcttgtcac atcgtcccca aaccggcttg ggggagatgc cgaaccgggg
     2821 tgccagcggc attgttggtg gctcagacat tacctttctg cgccatcgcc gattagtaaa
     2881 gagtctagtc tagcgtgcgg acttgcatcc ttcatataga cccacatttc ctttttgaac
     2941 tctgtgtaag tggttgtatg atcgcgccaa cgccagtatt catgcggttt tgacggcgca
     3001 aaggatactt cggactcgtc gtatctttcg atatctcccg cgcccggcag cgcgggcgat
     3061 aacggaacca tcgtggtgga attcaacggg atcgtttcgc agagtctggc gaaaaacggc
     3121 cagccggtgg agttgctggt gcagatgtcg cgcggcgtgc gggtggtgaa tctcggcgtg
     3181 cctcccgatc cgaaggtgga ctcgaaggga gacgtggaaa attacggagt gactgaaatt
     3241 aataattgcc cgagcgccgt cgacccctgg tatcaggtgt tcggcacttt caatccgctg
     3301 tggaatgtgg atccgccgga gtcctgggtg gatacggtcg accaggtagc cgtgttccaa
     3361 agcattctaa tcacggtcgt gggtgtagag accggtgaac tggtcacttt caatcaggcg
     3421 atcgacggcg gcttgagcgt ggtaacggcg aacgccgcgg gcgtggccgt gattcccgcg
     3481 ctgcttgccg tgcgaagcgt gaacgagaac gccgcgctga cgcgcgcgaa tcgcacggcg
     3541 cttgggaacg taggcacagc gggcgcgatc tttcagcgtg tggcgatgct gcagacgccc
     3601 ggcgcgattt cgcatcagct ttcaggcgat tccttcagcg cgacgatcac cacgacgttt
     3661 gcaaacggcg ccacgcaggt gatgagcgtg agcgatcttg gcgtcgtgca attgtccgcg
     3721 gcgcaggtag gggcgagtgc gagtgcgcaa gcagcgatta gaccgaggtc cccggcagct
     3781 gacgcgtctg cgtcaccgtc cgcgacggtg gacccgcaat ggccgattca aattccgggg
     3841 ctggtgaaag tgctgcccgt tccgggacta cagaatgcac cggttgcgat tgcggagctt
     3901 tccgatggga cgaatctcgt cttgtcgcgc gagccggatg gctgcgttcg catggccggg
     3961 atggttccgc gatggttgga tatgcccgcg gtttcaggcc gctgggcgat cagttcatcg
     4021 acggggaatc gcgtggcggt gttcgccgtc cggccattcg gttcgccgaa atgttgttgc
     4081 gggtgccaca ccgcggagca agcggcgcgc cgataacggc agaacggcct agcgttcggc
     4141 gagggcatgt gttcaacgcg acggtggttc cgcagggcgg cctggactac ctgacgctgt
     4201 ggcccgacgg cgaaccgaaa ccgctggtct ccaccttgaa cgccttggat ggatcgatca
     4261 ccaacaacat ggcaatcgtg cccagcacca acggaaaggt cgatgcttat gcgtctggga
     4321 tcacgcaact gatcctcgtt caatgagttg tggctctttt caagggagcc catatcgtaa
     4381 acaatccgaa gcggggggca tcgggccgga tcacttgtga aatggggatt ttacgtaagt
     4441 tatggcaaaa gatcgaacca ctagttcagt tgaccgaggc ctcgtttgcg agttagcttc
     4501 aaaactcgct acccgcttcc aagcaaagga ggtttcatta tgcaaaaccc agaatctgag
     4561 agcggatttc tctttcgtct cccttttgct gcgatggcca tcatgtttgc tctcgccgtt
     4621 gccgcgaccc agtcggcaca agcgcagacc tttaccgact tgcacagctt caccaaccac
     4681 ggcgacggga actcgccatt cgcgggtccg actatggaca gggcgggaaa tatctacggc
     4741 gtcacccttt acggcggtgc cggacacggt acggcgttca agctaagcaa gcgggccggc
     4801 aactggatcc tcgacacgtt gcacaccttc accggtggca gtgacgggga ctcgccattc
     4861 gggaatgtca cccttgggcc ggacggcaat gtctacggta cgaccatctt cggcggcggt
     4921 ggctgtacga gcggcggttg cggcacggta tttaagctgt ccccgccgcc ccgcatttgc
     4981 acccacaccc aatgcccctg gacggaaacc gtgatctatc ggttctccgc gagcgatacc
     5041 ggtcttcaca atccctgggg aggcgtgacg ttcgattcgg ctggcaatat ctatggcacg
     5101 acgtcggcgg gcggaacggg cgattgcagt ggtcagggct gcggcgcggt ctataagcta
     5161 actccttcgg gcggtgcctg gacagcaagt gtgatctaca acttcaccgg tttcgaagat
     5221 ggcgagtttc ccatggcaac catgaccatc gatccagccg gcaaccttta cggaacaacc
     5281 tactacggca acagcgtttt tgagctggtt cggtccggat cgggctggac ggaaaatacc
     5341 ctgtacacct tccaggatgg cgccgacgga ggaaatccgc aggcgggcgt aatcttcgac
     5401 agtgccggaa atctctacgg cgccactact acggtggacg gaagtccggc cggtggcgtt
     5461 gtttatgagc tgacgcattc cccgggtggc tggacgtatt cggtgctgta cacctacaac
     5521 cactccggtg gactgttcgg caatgtcgcc atcgatagcg ccggcaatat ttatggcacg
     5581 gccgtgaccg gcggaatcta tcaaggcgga tcggtcttca agatgaccaa ctcaggcggt
     5641 gtctggagcc tcaccgacct ccacgacttt gggggaattg gatggccgga gggcaacgta
     5701 ctcctcgacg ccagcggcaa cctgtacgga acggcaatcg gaggaggaca gtatggctgg
     5761 gggtcggttt gggagatcca gccctgagcc gaaggacaac ttctaattct gcacacaaac
     5821 agaccgtccc accagcaagc ctgagatgtg gatgtaagcg tgattacttg tgtaatggcg
     5881 atttatcgac agccatgctc agtcaacaac gaaagcggcg ctgataagtt cgacgagttt
     5941 ctggtcatcc gcgacacaac gaggcgtacc ttgtctccag tccgaagcag ttgcgtcgat
     6001 ggtacgaatg gtgcggtgtg caagtgctgg gtgggccaca ataaccgccg tgtgaaccgc
     6061 gcttcgacga ctcccagcgg aacacgttcg catcccgtcc gcagggcgac ggtgtcccaa
     6121 gtgccacgtc ctacatccca tctttgtatc tcgtcactaa cgacaatatc ccatagagat
     6181 cgtcctgcct agcgatcttc tttctgctct accccactct caaccctagt actgccaaca
     6241 aaacaagata cttaacgcac ttccctcctc ggccaactga cgtatgcgtc atcggtggcg
     6301 tcttggcttt caacacaaca cgacatgggc ggactgcccg gaaaggacca ccgcaatgtt
     6361 tggacgaatg atggttgaac ccgttgtgca gctcgccccc ggcgtcgatc tacagacagc
     6421 gatagccaca ctgataaaag ttgctgaaaa cgtcgagatc gttcgttgga tcgagttgcc
     6481 ggcgtcggtc ctgttgttct tgctcgtgcc cggagacccg gattctgggg cggtctatgt
     6541 cctcgaccgc aacaaaggaa cctggtacgc cgtcgacttc gaggacgaac aattcggcgg
     6601 gtacagcgca agccagctcg aaatgctcct gaaggagtgt aacttcctcg atcttattga
     6661 gcggccaggg ctttggcgct ccggtttgcg gtgggtggta atgcccggaa tgcggcctga
     6721 gacggccgtt tgacagaaaa aatattccgg gttatctaac gacacccaca tccgaaaacc
     6781 gtgtgggtga caggcgacca cgagcgcgag gacacgcgaa tctgccagaa ggaggtgacg
     6841 ggtcaaatgg caacgacaat cacggcaacc cggcaggttg ttgaggatta ccaaaggctg
     6901 ttcgtgaacc gtcgcgcgta cacgatgcag tccatgcggc cgcacccgga aacagggcgg
     6961 cattactact tccgggcaag caaaaaggga acagacctac ccccatttgc tgacgggaga
     7021 aaccatccgc cagcacctgg aaggcaaaat cacggtgggc ctgtatgcca tcaacccctc
     7081 gacccaacgc tgtaagtggg tcgccatcga cgccgactac aagaactcca tggaggacct
     7141 actcaggctg cagtaccacc tgacacagga caagttggaa ccggcgctgg aaatgagcaa
     7201 gcacggtggg catctttgga tcttcctggc tacgcctctg ctggccaaag agtgccgaat
     7261 ctacatccat gatcttgccc tgaggttggg agtgccggta aaaggctcag ggttggcgga
     7321 gggcatcgag gtgtttccga agcacgacgc catcggtgcc ggagagtttg gcaacgcgat
     7381 tcgcggaccc ttgggaatcc accgaggcgc caatcgccga ttttggattt atggcgccga
     7441 ttacacgctg gaggaccaga tcgcatacct caaccgtctg cgaaaagtca ccgaggcaga
     7501 gctgcggagg ttcatcgccg gaaaggaaag gccagagccg gatttctcgc cgcggccgga
     7561 gagggtcact tcaacgctac gaaaaactgg gcgcggacaa tcagaattcc gcattttgga
     7621 acacgtgggt accgtgcgga aggtcggccg gaattacgtc gcacgctgtc catcttgtgc
     7681 ggactgtgga cacgaccgga gcggtgacaa cctagccatc ctgatcgaag atccccgatt
     7741 ttacaagtgc tgggctggct gcaccaagga gatgattcgt gctgccctcg gctgcccgat
     7801 tcgtgtacgg cattccgcct gacacaagga gagttttatt gtcatgaacc agaagcgatt
     7861 ttcagatgca ctggggctga ggctaacgcg gcaagcactg cgggtgctga aggaacgcgg
     7921 aatctttgcc cattcgccag taagcctgga acaccagcac cttgcgaaac ggtatgtggt
     7981 gcgcgggttg gagtcaggag gcgcggccgg agaacttggg cgatacgtca ctttcaccca
     8041 ggaagatggt cagccaatcg ag
//
LOCUS       DASRKK010000334         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1296179, whole genome shotgun sequence.
ACCESSION   DASRKK010000334 DASRKK010000000
VERSION     DASRKK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296179"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="hslU"
                     /locus_tag="VFC15_07215"
     CDS             <1..391
                     /gene="hslU"
                     /locus_tag="VFC15_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HZM09983.1"
                     /translation="PSDLIPELQGRFPIRVELQSLTVEDFIKILTEPKSSLTKQYIAL
                     LDTEGLKLEFTPEALAEVANFAFRVNEGTENIGARRLHTIMERVLDEISFEAPDMKEK
                     SVTIDADYVRKTLTDIVKDQDLSRYIL"
     gene            complement(420..872)
                     /locus_tag="VFC15_07220"
     CDS             complement(420..872)
                     /locus_tag="VFC15_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09984.1"
                     /translation="MPVTRILALLLLALASQSTIAQVTQSPTTNAVSNKTSPSKTPSD
                     QTKFVLSGDGKEYHSEQLITPSDTVGITDKIQVQVGRINGKPAYQAIVVKPTNAPKNS
                     KEVDELLRTSPVMAAAQSDMQQLCTGEKVCVETKKLNGHDVCVKWRCK"
     gene            complement(1843..>3235)
                     /locus_tag="VFC15_07225"
     CDS             complement(1843..>3235)
                     /locus_tag="VFC15_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09985.1"
                     /translation="DQQAAKKSDKKSKRGPRAIAVVEFLPGGGTRLVPIALWIDGRFY
                     DASLYGAHPEPMALEPETLYQALAYGEPAGWFTVTTPRDVNGNWIADGQWKPAQPLEV
                     RLAEQAAKQPKPKPKGHDVLSDDQGPPVLRRPGSSDSSSAGGGQGSAPASTSGGNSTS
                     SASGSSTASSNAPPKPDDDSDRPTLKASPSAASSAPSDSKPTLGDNTQQAKAPAVPSN
                     APPASSSNEDDPDRPVLRRGKPVEQPTVDSTNTPGSNATVSKAAKPLASSGATAAPGA
                     AAKATQAPRAYAAISDAGNYETRSLLYGMAAGERQSLGEPMSKMAMDEVRKYASKHNG
                     PPPPKTATITDYDLRGFDLDYSNSATLVLSAKLPVAPAKATSAAEFDYFVTVVARVDV
                     NGTPQKIFSVVTDRNHLDAYPRMQIIDALDADANGRGDLLFRQYSDTGISYALYRVFP
                     YQMEKIFEGGAGM"
BASE COUNT          682 a          914 c          922 g          717 t
ORIGIN      
        1 gcccagcgac ctgatccccg aattgcaggg gcgcttcccc atccgcgtgg agctgcaatc
       61 gctgacagtg gaagacttca tcaagatcct caccgagccg aagtcgtcgc tcaccaagca
      121 gtacatcgcg ctgctcgaca ctgaaggact gaagctggag ttcacgccgg aagcattggc
      181 ggaagtcgcg aacttcgcct tccgcgtgaa cgaagggaca gagaacatcg gcgcacgtcg
      241 cctgcacacc atcatggagc gcgtgctgga cgagatcagc ttcgaggctc cggacatgaa
      301 agagaagagc gtgaccatcg acgccgacta cgtgcgcaag accctcaccg acatcgtgaa
      361 ggaccaggac ttgtcgcgct acatactgta actcgctggc gctcgcatgg ctatttaatt
      421 tacttgcatc tccacttcac gcaaacgtca tggccgttaa gtttcttggt ctcgacgcag
      481 actttttcgc cggtacaaag ttgctgcatg tctgattggg cagcagccat gacgggcgag
      541 gtgcggagaa gttcgtcgac ctctttcgag ttcttaggcg cgttagttgg ttttaccacg
      601 attgcttgat atgcgggttt gccattgatc cttccaacct gaacttgaat cttgtctgtt
      661 attccgacag tgtcggatgg tgtgatcagc tgttcggaat gatattcctt gccgtcgcct
      721 gaaaggacga acttcgtctg atcactcggc gttttggacg ggctggtttt gttgcttacc
      781 gcattggtcg tcggagactg tgttacctgc gctatcgtcg attgacttgc caaggcgagt
      841 aggagcagcg ccagaattcg agttactggc attattttgg gcctccttgc gatacgcaag
      901 ctttagcagg actagaaaaa atagtaaaca aaaaagcgaa agtattgcgt aatcaacgat
      961 cgtcggaaag atgctaacgg atgtcggcac tatcagcgcc cgaagacccc agagcgcggc
     1021 aaaaaaacca attgatttct gaagtaaatc cttcgcatct cctattgcca cgaaagacac
     1081 caagaaaact aacgccagga ctagaaatat cacgctaaca agccgcacga ataatcgtcg
     1141 atgcaacttc agtacgattc caccccctgc agctgaatcc cggttgacta tgagattctg
     1201 atccgcaagc tcgacttcaa cggattcaaa cgaaatttga cctgaaccgc ctaaacaagc
     1261 accagccgga tcattcgcgc aggcccgggg atttattgat acttcatagt aatcgaacgg
     1321 ataccaaatc tcgcgcctgt gacggacgat aggaaggccc aattcgccac agttcagttg
     1381 tagccaagag tgcctcgttc cttttgcatc attcacctca taagtagccg gggctgagca
     1441 gtcgttcgtt gtgtccccct tagtccattt aagccactta gtatcatctg ggtcgtaacg
     1501 tggagtaaag gaaatagtcc ttatgtcacc gcttgccacc gatgtaattt ctaatgtctc
     1561 cgtcgacctg attgaccgcg acaatgtgtc atacttcagc gctcccaaaa gcagacgacc
     1621 tgtaaacttc gcgtttgata cggggtccgg tgctatcttt tgggtatcgc caaactcctt
     1681 ccaacccaag tcatacacga gcagaataat taccaagcaa aagagaagcc aaagaagctt
     1741 tatcacagtc cgcatggaga ttaccgaggc actcactcag gattgcggat tatgagtata
     1801 tctcatgttg tagaggagac attcaggcgc cagtctgccc acctacatcc ccgccccgcc
     1861 ctcgaagatc ttctccatct ggtaggggaa cacgcgatag agagcgtagc tgatgccggt
     1921 atccgagtac tggcggaaga gcaggtcgcc acggccgttg gcgtcggcgt ccaaggcgtc
     1981 gatgatctgc atgcgcggat aggcgtcaag gtggttgcgg tcggtaacca cgctgaagat
     2041 cttttgcggc gttccgttga catcaaccct ggccaccacg gtgacgaagt agtcgaactc
     2101 ggctgcggac gttgcctttg caggcgccac cggcagcttg gcactcagca ccagcgtggc
     2161 ggagttactg tagtcgagat cgaagccacg cagatcgtag tcggtgatgg tggcggtctt
     2221 gggcggcggc ggaccgttat gcttgctggc gtacttgcgg acctcgtcca tcgccatttt
     2281 cgacatcggc tcgcccaggc tttgcctttc cccggccgcc atcccataga gcaaggagcg
     2341 agtttcgtag ttgcccgcat cggagatcgc agcataagcc cgcggcgcct gtgtggcttt
     2401 ggccgccgca cccggagcgg cggttgcacc gctcgatgcg agtggtttgg cggccttact
     2461 tacagtcgca ttcgatcccg gtgtgttggt cgagtcgaca gtcggctgct cgaccggctt
     2521 gccacgccgt agaacgggac ggtcgggatc gtcttcgttg gatgacgacg ctggcggggc
     2581 gttggacggc acagctggcg ctttcgcttg ctgggtgttg tcgccgaggg tcggcttgga
     2641 atccgaaggc gcagaagaag cggcagacgg cgaggcttta agcgtcggac ggtcagaatc
     2701 atcgtccggc ttcggcggag cattgctgct ggcagttgaa ctcccgctgg cggaagaggt
     2761 cgaattccct cccgaggtgg aagctggcgc gctgccctgc ccaccgcccg cagaagacga
     2821 gtcgctcgat ccagggcggc gcagcactgg cgggccctgg tcatcgctga gcacgtcgtg
     2881 ccccttgggc ttcggcttgg gctgcttcgc ggcctgctcg gcaagcctga cttcgagcgg
     2941 ctgtgccggc ttccattgtc catccgcaat ccagttcccg ttgacgtctc gcggcgtggt
     3001 gacggtaaac catcccgcag gctcgccgta agccagcgct tggtacaggg tttcaggctc
     3061 cagcgccata ggctcgggat gggcgccgta aaggctggca tcgtagaagc ggccgtcgat
     3121 ccatagtgca attggcacca gccgcgtgcc gccgccgggc agaaactcaa ccacggcaat
     3181 ggcgcgcggg ccgcgtttgc tcttcttgtc gctcttcttc gccgcctgct ggtcc
//
LOCUS       DASRKK010000335         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_318999, whole genome shotgun sequence.
ACCESSION   DASRKK010000335 DASRKK010000000
VERSION     DASRKK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318999"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFC15_07230"
     CDS             <1..1033
                     /locus_tag="VFC15_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM09986.1"
                     /translation="FLDLLHNPEFRPDKLELAKEQMYTEISRRNDNVDSIVHRQSRII
                     AYGKDNPYARVPEFATVAAVTREDLLNWHQQYVYPNNIIFGITGDFDAKAMEAKLRQA
                     LGSWQKGPDAKAPDIKFTEPKPGLYFVRKTDVNQSSIDMLDLGIERSNPDYFAVTVMN
                     EIFGGSFSSRLFNHLRSGKGLAYGVSGGVGYSWDHPGVTNVEMQTKSASTVEGIQGLD
                     EEIDGLLKNAATPEELKRAKDDILNSFIFQFDTPEKVLREKMAYEFYHYPLDFLERYR
                     SEVEKVAADDVSRVARKYVHKDRMAVLVVGNDTEFGKPLSTLGPVQDVDITIPPPPAS
                     LMQQGPGGQ"
     gene            complement(1121..1387)
                     /locus_tag="VFC15_07235"
     CDS             complement(1121..1387)
                     /locus_tag="VFC15_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09987.1"
                     /translation="MKLDAEEREILASYERGEWRPVKGKRGDRNQYARYAKATFRKDR
                     RLNIRISSKDLEAIQKRALEEGLPYQTLISSLLHKYVSGRLREA"
     gene            complement(1371..1670)
                     /locus_tag="VFC15_07240"
     CDS             complement(1371..1670)
                     /locus_tag="VFC15_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM09988.1"
                     /translation="MKYFTWDDAKNEKLKAERGIGFEDIVFLIERGDLLEIVEHPKQE
                     RYPRQQMFIVKREDYVYLVPFVEEESRFFLKTIIPSRKATREYLRKEAEDDEARR"
     gene            1772..2491
                     /locus_tag="VFC15_07245"
     CDS             1772..2491
                     /locus_tag="VFC15_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM09989.1"
                     /translation="MSPAAQRKIPPGQHLLIDADDTLWENNVYFERAIADFISFLNHH
                     EHTPDQVRQVLYDVERENIRTHGYGMHSFALALVRCFEQLSVESLTPALHETIRGFAY
                     AIAEHPMEILPEVPETLADRAQRHHLFLVTKGNIGEQTGKVERSGLKQHFTAIEVVAE
                     KDAATYRAVVEKYGLLRESTWMIGNSPKSDINPALEAGLHAVFVPHHNTWMLERDKLR
                     PEAGDGRLLQVESFGHLRDLF"
     gene            complement(2789..>3431)
                     /locus_tag="VFC15_07250"
     CDS             complement(2789..>3431)
                     /locus_tag="VFC15_07250"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZM09990.1"
                     /translation="GTARYLHRCERHGQQISMPGETAPALLLKDRSPVLAVAGVVHPR
                     LVISRRVMRGLTAEQIDAALRHERAHRSSGDNLKRFLMLLAPDVLPFMRGFVTLERSW
                     AKAIEWAADDQASAGDPRRALSLADALVRVARMGNKPQLSYLTSSLMADDHDLSERVD
                     RLLRPQPIPEKPAKELIPLVSGAAALIAASLMTVVLWPASLSMVHQALEQLIH"
BASE COUNT          743 a         1012 c          987 g          689 t
ORIGIN      
        1 gtttctcgac ctgctgcaca atcccgaatt ccgtccggac aagctggagc tggcgaagga
       61 acagatgtac acggaaatct cgcgccgtaa cgataacgtg gattccatcg tgcatcgcca
      121 gagccgcatc atcgcctacg gtaaagacaa tccctacgca cgtgttcccg agtttgcgac
      181 ggtcgcagcg gtcacccgcg aggacctgct gaactggcac cagcagtacg tttatcccaa
      241 taacatcatc ttcggcatca ccggcgactt cgatgcgaaa gcgatggaag ccaagctgcg
      301 tcaggcgtta ggttcgtggc agaagggacc agacgcgaag gcccccgaca tcaagttcac
      361 cgagccgaag ccgggattgt atttcgtccg caaaaccgac gtgaaccaga gcagtatcga
      421 catgctcgat ctcggaatcg agcgcagcaa tcccgactac ttcgccgtga cggtgatgaa
      481 cgagatcttt ggaggcagct tctcctcgcg gcttttcaac catctgcggt ccggaaaagg
      541 actcgcctat ggcgtgagcg gaggtgtggg gtatagctgg gaccatccgg gcgtgaccaa
      601 cgtcgagatg caaaccaaga gcgccagcac cgtggagggc attcaggggc tcgatgagga
      661 gatcgatggc ttgctcaaga acgcggctac tccagaggaa ctaaagcgcg ccaaagacga
      721 catcctcaac tcgttcatct tccagttcga cacgccggaa aaagttctgc gcgagaagat
      781 ggcctacgag ttctatcact atccgctcga tttcctggag cgctatcgca gtgaagtgga
      841 aaaggtagcc gccgatgatg tctcgcgggt ggcgcgcaag tacgtccaca aggaccgcat
      901 ggcagtgctg gtggttggca atgacacgga attcggcaag cccctcagca cactcggccc
      961 ggtgcaggac gtggatatta ccatccctcc gccaccggcg agcctgatgc agcagggacc
     1021 gggcgggcag tagggtgatc gacacactcc cactccttca caaacagggc gagtgagtgg
     1081 gcaaccgcac ggccgaggaa gggttaggcg cagagatcga ttaagcttcc cgcagccgtc
     1141 cggagacata cttgtgcagt aggctggaga tcagggtttg atacggaagg ccctcctcga
     1201 gcgcgcgctt ctggatcgcc tccaggtcct tgctcgagat gcggatgttg aggcggcggt
     1261 ccttgcgaaa tgtcgccttg gcgtagcgag cgtactggtt ccggtcgccc ctttttccct
     1321 tcaccgggcg ccactcccct cgctcgtacg atgcgagaat ctctcgctcc tcagcgtcga
     1381 gcttcatcgt cttcggcctc cttcctcaga tactccctgg ttgccttgcg gcttggaatg
     1441 atcgttttca gaaaaaagcg gctctcttcc tcaacgaaag gcaccagata gacgtaatcc
     1501 tctcgcttta cgatgaacat ttgctggcgt gggtaccgct cctgttttgg atgttcaaca
     1561 atctccagta ggtctccacg ttcgatgagg aaaacgatat cctcaaagcc aatgccgcgc
     1621 tccgccttca gcttctcgtt ttttgcgtcg tcccaagtga aatacttcac ttacttgcag
     1681 tgtagcacga cgtgtgcctg ataggcacac attgacatct tctacatcgt tccccttttg
     1741 cggacggttc aataaacgat aagatgcggt catgtctcct gcggcacagc gcaagatccc
     1801 ccccggccag catctcctga tcgacgctga cgacacgctc tgggagaaca acgtttactt
     1861 cgagcgggcg atcgccgact tcatctcatt cctgaatcat catgaacaca cgcccgacca
     1921 ggtgcgccag gtgctgtacg acgtggagcg cgaaaacatc cgcacccacg gctatggtat
     1981 gcacagcttc gcgctggcgc tggtcaggtg cttcgagcag ttgtcggtag agtcacttac
     2041 tcccgcattg cacgaaacca tccgcggctt tgcctatgcc atcgctgagc atcccatgga
     2101 gattctgccg gaggtgcccg aaacgctggc cgaccgcgcc cagcgccatc atctcttcct
     2161 ggtgaccaag ggcaatatcg gcgaacaaac gggtaaggtc gagcgctctg gcctcaagca
     2221 acatttcacc gccatcgaag tcgtggcgga gaaggatgcc gcgacctatc gggcggtggt
     2281 cgagaagtac ggcttgttgc gcgagagcac ctggatgatc ggcaacagcc ccaagtccga
     2341 catcaaccca gcgctggaag ccggactgca cgccgtgttt gttccccacc acaacacctg
     2401 gatgctggag cgcgacaaac ttcgtcccga ggccggcgat ggccgcctgt tacaggtcga
     2461 gagcttcggc cacctgcgcg acctcttctg aggttaggac tgcccaatcg taggtggcta
     2521 aggcacgttc agagttagta tgaacttcag acgtttcgac tctggaattc ttgttccggc
     2581 ctctggaatt gtcattacga gcgaagcgag gaatctgctg tttgtttgcg cctagaacga
     2641 cggccggggg caacagcaga tccctcacgg cctaaaggcc gttcgtgatg acaactcagg
     2701 gatgacacaa gagccggaaa ctcttgtccg ggcaaaaata atgcccagcg ttcaagccgg
     2761 gcattacggt gtggagtagc aaaatttatt agtggataag ctgttcgagg gcctggtgca
     2821 ccatgctcag gcttgccggc cagagaacca cggtcatcag cgatgcggcg atcagggcgg
     2881 cggccccgct aaccagggga atcaattcct tcgccggctt ttcgggaatg ggctgtggac
     2941 gcagcaagcg atccaccctc tcggacagat cgtgatcatc ggccatcaag gagctagtca
     3001 gataagaaag ctgcggcttg ttgcccattc tcgccactct caccagggcg tcggccagtg
     3061 aaagggcacg acggggatcg ccggcgctgg cttgatcgtc agccgcccat tcgatggctt
     3121 ttgcccaact ccgttcgaga gtcacgaaac cgcgcatgaa gggcagaacg tcgggcgcca
     3181 gcaacatgag aaaccgtttg agattgtcgc cggatgagcg atgggcacgc tcatggcgca
     3241 gggcagcgtc aatctgttcc gcggtgagtc cgcgcatgac gcggcgcgat ataaccagcc
     3301 ggggatggac cactcccgcc acggccaaca cgggagacct atctttaagc agcaacgccg
     3361 gcgcggtttc tcccggcatg ctgatttgct gcccgtggcg ctcgcagcga tgcagatagc
     3421 gcgccgtacc g
//
LOCUS       DASRKK010000336         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_710439, whole genome shotgun sequence.
ACCESSION   DASRKK010000336 DASRKK010000000
VERSION     DASRKK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710439"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(604..2625)
                     /locus_tag="VFC15_07255"
     CDS             complement(604..2625)
                     /locus_tag="VFC15_07255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM09991.1"
                     /translation="MKSANRQPLVFWGVAVLLLVIVLQLVLMARDNSATFDEPFHIYA
                     GYLQWKHGYVLLNPPLITSFFTLPLLGMNLPEPPITSDLPGGERTYDNLEFQGGKALV
                     FQNDTNAILFRTRMTAMVFTVVLALLVFAAARDMFGVGAGLIALGLMAFDPTLMGHSA
                     LATLDAGNACLMFWAIYAFYRYVKSPTAWRLIATGVIVGLALAAKHSAILLFPTMGVL
                     AIIEVVWRGKLELELAAPPVRAGRHALRLALALTVILAISMTILWGSYGFRYAPGDAV
                     PFNPPMKTQLDRVPSALEGRLLGEIDGLHLLPAPYTYAFANILRQAKSYTSYLMGVAY
                     PHAVWFYFPITLLIKSSLTFLILLVISAGAVAIGAVRLSRGILYMGISALVFMAFAVT
                     GGMNIGVRHILPVYVFLSVPIAGASWSLMQRNRSWIYAVVALLVFQAGSVLHAYPAYI
                     SYTNEVFGGPANSYKYVSDSSSEWGQQLNAVRRYVDARGIKNCWFAYFNQAMMDFRSF
                     GIPCTPLLTAEYQTPDTPPAIDGTVLISAAVLSGFETGTGPLNPYGQFQKLKPAAVID
                     YGVFVYDGHFEIPLAAALRHVQKAGGLLSGKNAPDALSEARQAEALAPDSAAVNDMLG
                     QAAEASGQPDDAARYYQKALTIAKSMQPGFQEARIADLEKRLGAGKQGQ"
     gene            complement(3085..3417)
                     /locus_tag="VFC15_07260"
     CDS             complement(3085..3417)
                     /locus_tag="VFC15_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09992.1"
                     /translation="MTTGSTMRWYHYVAYFFGGAFLANTLPHLMNGISGHAFQSPFAS
                     PPGEGLSSATVNVLWGLFNLAAGYVLVCCVGRFDLRRTLHVAVLGAGILIMSLMLAHA
                     FGRFYGGL"
BASE COUNT          911 a         1229 c         1292 g          771 t
ORIGIN      
        1 cggcgagccc caggtcggcc aaccaaactt cacctggttg tgggctcatc gagcaggaaa
       61 ggtttccggt tctcggggct ggaaatgatt ggaatgaaag aggaaaagaa agttgaagag
      121 gccgtgatgg acctgcaaac tcaatgcgag ttcactcaat cttgcaggtg gctcgcgtgg
      181 ctggcggacc tggcgagcac gcgcggttca tcctgtggac ggcgtttcac gggaggaatc
      241 tgctcagttt ccaggatgcg gaggggagag cggtgcattc tacgtgcatt ctacgataac
      301 gccgtctttt gtccagaact ctcgtttccc ggcaactgaa tgacagaccg ccatcggcaa
      361 aatgcacgat gacagcctcg cgaagttcgc cgcagcacga aggccgacga ccatcgcgat
      421 atttggcagc ccctccgagt tctttcctgc acatccgtgg ctacgagtcg aaataagggc
      481 actcgtcaga ttcacggagg ttcaaggttt tctgtgcatc gacgcaggcc gattggctgg
      541 cctcccgcgc ctctcggagg ccaccaatgc cgctccaacg cactgctggt ccgccgtctc
      601 gattcattgc ccctgcttgc cggcgccgag ccgcttttcc agatccgcaa ttctggcttc
      661 ctgaaatccc ggttgcatgg acttcgcaat cgtcagcgcc ttttggtaat aacgggccgc
      721 gtcgtcaggt tggccgctcg cctcggccgc ttgtcccagc atgtcgttga cggctgcgga
      781 gtccggcgcc agcgcttcgg cctgccgcgc ttcggaaagc gcgtccggcg cattctttcc
      841 acttaggaga cctcccgctt tttgtacatg tcgcagcgcc gccgccagcg gaatttcgaa
      901 atgtccgtcg tatacgaaca cgccatagtc gatgaccgcg gccggcttca gtttctggaa
      961 ctgtccataa ggattgaggg ggcctgtgcc ggtctcaaac ccggaaagaa cggccgcgct
     1021 gatcaacacg gtgccatcga tcgcaggcgg tgtgtccggg gtttgatact cggccgtaag
     1081 cagcggtgtg caaggaatgc cgaacgagcg gaagtccatc atcgcctggt tgaaataagc
     1141 gaaccagcag ttcttgatcc cccgcgcgtc cacataccgc ctgacggcat tgagctgctg
     1201 gccccattcg gaactggagt cgctgacgta cttgtagctg ttcgccgggc cgccgaagac
     1261 ctcattggtg taggaaatgt aagcggggta agcatgcagc accgagccgg cctgaaagac
     1321 cagcagagca actaccgcgt aaatccagct ccggtttcgc tgcatcagag accaggatgc
     1381 cccggcgatc ggaacggaga ggaagacgta gactggaagg atgtgccgca cgccgatatt
     1441 catccctcca gtcacggcga aagccatgaa gaccaaggcg gatatcccca tatagagaat
     1501 cccccgcgag agccgcacgg cgccgatggc gaccgcaccg gcgctgataa ccagcaggat
     1561 gagaaacgtt aggctcgact tgatgagcag cgtaatgggg aagtagaacc agaccgcgtg
     1621 cgggtaagcc acacccatca ggtagctcgt gtacgacttg gcctgacgaa gaatgttggc
     1681 gaaggcgtag gtatagggcg cgggcaaaag atgcagcccg tcgatctcgc cgagcagtct
     1741 gccctccaag gcggatggca ctcgatcaag ttgcgtcttc atgggaggat tgaacggcac
     1801 agcgtcgccc ggagcgtagc ggaagccgta ggagccccaa aggatcgtca tcgagatcgc
     1861 aagaattact gtcaacgcga gcgccagacg caaggcgtgc ctgccggcgc ggacgggtgg
     1921 agcagccaac tctagctcta gctttccgcg ccaaacaacc tcaatgatcg cgagcactcc
     1981 catcgtcggg aacagcagga ttgcgctgtg tttggcggcg agcgctagcc ccacgatgac
     2041 accggtcgcg atcagccgcc acgccgtcgg tgacttcacg taccggtaga aggcgtaaat
     2101 tgcccagaac atgaggcatg cgttccccgc gtccaaagta gcgagggcgg aatgccccat
     2161 gagtgtgggg tcgaaggcca tgaggccgag cgcgatcaac ccggcgccca cgccgaacat
     2221 atcccgcgcc gccgcaaaca caagcaacgc caacaccact gtgaagacca tagcggtcat
     2281 ccgcgtgcgg aacagaatgg cattcgtgtc gttctggaag acaagagctt tgcccccctg
     2341 aaattccagg ttgtcgtagg tgcgctcccc gcccggcaga tcgctcgtga tgggtggttc
     2401 cggcaagttc atcccgagga ggggcagggt gaaaaaactc gtgatgaggg gcggattcag
     2461 gaggacataa ccatgcttcc attgcagata cccggcgtaa atatgaaacg gctcatcaaa
     2521 ggtagccgag ttgtcgcgcg ccatcagaac gagttgcaga acaatgacca ggagaaggac
     2581 ggcgacgccc cagaaaacga ggggctgacg attcgctgat ttcatgaagc ctccacaagc
     2641 cggcgcaacg gccaatccag ggaaggtcca agcgggacct ggatgattgg aagcgacagt
     2701 gtatcatggc gcttctttgg gcaccggagc gccgaacacc cacaagacga gcgatgaaac
     2761 gcgtgccgga tccggcccct taccgccgct cctctcactg gacggttaaa ggcagcctat
     2821 tgggaggccc gacggcctgg gcggtcggca cgatccccta ccggcaagcc tctcgccaga
     2881 cgttgcgaac cgagaccggg aggccttcaa gacgtccgcc cggactcggg aacgatatgt
     2941 gggcttgtcg gaaacttgac cgggaggaga ggcagcttgc acgcgatacg cgcgagggca
     3001 gtgtactttc gaccctatcg cccgtgcttt ctgcatcttc gccgcagcgc ggcggcggga
     3061 tccgatctta ttgaaaacgc cgggtcacag ccccccgtaa aaccggccaa atgcgtgcgc
     3121 gagcatgagc gacatgatca ggatccccgc ccccaggacg gctacgtgca gggtcctgcg
     3181 caaatcgaag cggccgacac aacagaccag cacgtagcca gcggcgagat tgaacaggcc
     3241 ccataggaca ttgaccgtcg ccgaggacaa gccctccccg ggcggtgacg cgaaggggct
     3301 ctggaaggca tgacctgaga taccattcat gagatgcggc agggtgttgg cgaggaacgc
     3361 gcccccaaag aagtacgcca catagtggta ccagcgcatg gtcgatcctg ttgtcattcg
     3421 ttgctcctgt tgtcattcta tgctgccgct gtgcgattcg ttcggcgaaa acgctgtacc
     3481 cccttcgacc tgctccgtca attttctgcc gaagaagacc tgcacctgat catgtggcgt
     3541 tcgggctctg aagtggcgtt atcgtagatt ggcccgtaaa cccatcggag gccgtgacgc
     3601 atatcagttc cgagagcgag cacgtcagtt gggagtggag tgcgtaacgc ggatggtcga
     3661 ggagtcgggc ccaccgggtc ggcccaagct cgactcgtag gggcgcctcg cgtacaacgc
     3721 caagacgcga aggcatgata ctcgattgcg ctatgtgcga aggctgctac gataccagga
     3781 cgaatggctg atccgatccg gttgaccaag ggtgtccata cgcgcgacat tcgaagctcc
     3841 ttgactccgg gcagaacaaa ccgagtgttt gccgctcttg acggacctcc gctaagggtt
     3901 ctcgaaaagt atccgcttga tgtgctggaa ctcgggcacg gtctccccag tgggtgggac
     3961 ggtggatagt tcccctcgga acgccggatt acggtgcggc cgtcagttcc ccgcgagcac
     4021 tttggacagg cggtttgctc ccggctctgt tcagttcgtt acagacgcgg cgaagtccga
     4081 gacggaggcc atccagcgga acctcgtacg tgagattggg catctactct tggagcagga
     4141 gtcgcgggcg gttaagcgct acgtgaaatc ggtgttccaa cgaaaccgag acgcagcagt
     4201 tag
//
LOCUS       DASRKK010000337        11008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_808034, whole genome shotgun sequence.
ACCESSION   DASRKK010000337 DASRKK010000000
VERSION     DASRKK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808034"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFC15_07265"
     CDS             complement(<1..1539)
                     /locus_tag="VFC15_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZM09993.1"
                     /translation="MHGTLLRSLSCSKAGSVLCLLVLLLSASPAAMGQEKLIAPVAYG
                     TISVVDPATLNINEMITAGAYQGFALVGANPRLGFVGANDYLSVIDFSLGREVNRIYG
                     VCPYQSAAFTSDQKYLLVEDGCGYGYFYGLTMVNATTGRVVRRVNLSTVLGSGTYSLG
                     SVVVAGKKAYVTSQSGDPALPPIAVLDLRTFRLRRVNVPSGYIGYSGQSTPNAAVTPD
                     QKYVVMVEDLNSDGSSHLYVINTANDQVVQDQPLSFDPAGLVITPVNTAGKVYGYLLA
                     YGTSGFSATVIDLNEGSRTFGQLLPQTEVSLRTYFLYPSTAVINSNGSRLVVGGEKTG
                     RDSPNPNVVELDTAQMLIDPSKAILGSATLGGGGTPHGFSIATISTTSPPTAPTVTRV
                     LPATITNNVDNTLTVTGTNFASGATVRIGTFPALPATVNSSTSLQVTVSKNFPAQARL
                     DIVVTNPRINGLPSQQYQSGILPASLTVYPNPQFQPGNQFAALNLSTYSVSVYEPGQQ
                     TMINV"
     gene            complement(1806..2174)
                     /locus_tag="VFC15_07270"
     CDS             complement(1806..2174)
                     /locus_tag="VFC15_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM09994.1"
                     /translation="MRSTGLSLYPGAPCRVIDTRQLGPPFIGTLSPPVDVVDSPCGPP
                     ATAQAYVFNATVVPSGAMGYLTLWPADIGRPLQFSTLNAIDGWITSNMAIVSTTNGKV
                     DAYASGLTQLILDISSYFAP"
     gene            2287..3423
                     /locus_tag="VFC15_07275"
     CDS             2287..3423
                     /locus_tag="VFC15_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM09995.1"
                     /translation="MLPLRSVWQRNFRPILTVLWELLRDGLQFLNVATRSRTAVAAEI
                     LFLRKQLAYYQDHQIRPRRLTEAARLSLVLWSRLFEWKEALMVVTPGTFVRWHRRAFK
                     LYWRWKSRGGRPPLPKDIRQLIARMVRENITWGEERVADELSLKLGILVSPRTVRKYW
                     PKQPDGTGRARTSSQHWRTFVKNHAQGIIACDFLVAVTARFQVLFVFVVMEVGSRRIL
                     HYNVTAHPTADWTLQQFREAIPSYRRYQFLIHDRDSIFSRDLDEELKGSFGLRILRTP
                     VRAPKANAYCERLIGTVRRECLDFMIPLNERHLRRSLQGWITHYNKGRPHSSLGPGFP
                     EKTMDRPLPQPRTHRHRLPRDCEIRVMDVLGGLHHEYWLDKIVA"
     gene            complement(3873..5219)
                     /locus_tag="VFC15_07280"
     CDS             complement(3873..5219)
                     /locus_tag="VFC15_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM09996.1"
                     /translation="MSPNPDAAVLPKPSVKLDPAHEYTLPELIDIAEREHQETRVAWQ
                     LARNAALAAGFAQSTYLPRVTANIVGGYQDSTGNVGTGRFTVSGTGSAAGTVSAISLE
                     WLLFDFGGRANVVSAAKKLAQASNITFTGVHQKVIHGVCIAYYALLAARQRVRTNEQA
                     LANALELQNAAEARYSGGVGTLLESSQARQVRAQIQLATIQAEGMERDMYATLLAAMG
                     LSPLEKIRIAPFEHRRLPPEILTPVEQVVKDALACRPDVLAAYAAEQASQASVKAAEA
                     LYRPKIFLAGTGAYASGQLGLTALPSIGDQLPTLNISGNQWSSAVLVGLTIPVFDGKR
                     REDVIQQAKNNTVKAAATLDRIRIDAIQEIVAAQNALRTSIAANQAAEVLQEAAKTTY
                     DAALDSYKQGVVSITSVIEAETQLLQANLATDDAYNSALSAAATLAFSTGSLGASP"
     gene            complement(5392..7332)
                     /locus_tag="VFC15_07285"
     CDS             complement(5392..7332)
                     /locus_tag="VFC15_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZM09997.1"
                     /translation="MASLADEEGSGIRLWRLLTERSPGRFAFAARLALICALTAVVAE
                     VYQTPEIALTIYIAFFLNKADRVSSMALTIALTIFATIVLSFLFLIASPVLANPSLRV
                     GTMAGVSFCLLFLVSASKLKPVGSDVALVIAYALDVLGSAPFGELATRALLYAWLFVG
                     IPAGVSAVVNLILAPSPLKILLRELALRLRTAGQALQGDTEAISQVRKLTAEGDAELQ
                     FQLKLSVIEHSSRRADLDALQGATDCVVAVLSAVQLMVSERDALPSRATREQMTHKLF
                     ELAQIFERNAYPAQVEPISNKEEESDLARSGIGLFNVGLKHFGKIMPAELPRKKEKSG
                     FLATDAFTNPEHVDFAVKTTAAAMLCYLIYTLLGWPGIHTAFITCYIVSLGTVGETVQ
                     KLTLRILGCLVGVGLGLFVMIGIIPRVTGIGHLALIVFGGVFIAAWVAVGSPQISYAG
                     FQMAFAFLLCVVQGPSPSFDMVIARDRVIGVLLGNFLSYLVATRLWPRSVGPRIDRAI
                     ETATQQLEAIVRSSDQWHRNRLFAETSTLLNGVEDDIQLAAYEPESIRPTRASLSLRL
                     DVVHVAQSLQTPLLAASETFDDRSRQGIIEMFQGKQSEEQRDLSLARRTGRGAHLKEL
                     IVARSNLFREALRRLHQAEEHA"
     gene            complement(7313..8374)
                     /gene="mdtN"
                     /locus_tag="VFC15_07290"
     CDS             complement(7313..8374)
                     /gene="mdtN"
                     /locus_tag="VFC15_07290"
                     /inference="COORDINATES: protein motif:HMM:NF007785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtN"
                     /protein_id="HZM09998.1"
                     /translation="MGSVLMTEAHGIRKRKPLGAVLATVIVGTAIALWFWVESRTTLS
                     TDDASIDAEVVHVASPVGGRIIELPVHENEYVHKGDLLFRIDPTPYENTVTAARAQFD
                     LARAALGSKRRLVSTERSNAEIASDQIRRAQSNLALTLRTEERLKPLTAKGYVPRQQL
                     DQAEVAGQDAGISLSESEKQEQAAHGLIDNIDAADAGVRAAAATLANARRALEDTAVR
                     APHDGRVVGLNISTGEIVVPSQSIFTLINTEEWFVSANFREFDLHLVKPGDCVTAYSM
                     INRRVPMKGIIQSVGFGVLSDDRINLPRFMPYVQPSLNWVHVAQRFPVRIRLENPPQT
                     LVRVGASAIVEVDHGVACR"
     gene            9526..10524
                     /locus_tag="VFC15_07295"
     CDS             9526..10524
                     /locus_tag="VFC15_07295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM09999.1"
                     /translation="MDYVNLGKTGLKVSRICLGCMTYGVPATGALRPGSHAWALNEEE
                     SQPFFRQALDLGINFFDTANVYSGGSSEEVLGKFLKANTRREAVIIATKVHGVMRDEP
                     NGRGLSRKSIFYELDQSLRRLGTDYVDLYQIHRWDYETPIEETLEVLHDVVKSGKVRY
                     IGASSMFAWQFTKALYLADLHGWIRFVSMQNHYNLLYREEEREMMALCRSEDIGVIPW
                     SPLARGRLTRPWQSETTKRYETDQFGKKMYLQTEEADRKVVDQLGQIAERRGVPRAQL
                     ALAWLLSKPGVTAPIVGATKAQHLQDAVAALSLHLTPEEIASLEEPYTPHPVLGFS"
     gene            10564..>11008
                     /locus_tag="VFC15_07300"
     CDS             10564..>11008
                     /locus_tag="VFC15_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM10000.1"
                     /translation="MQLSKRPLGSSGVMITSVGFGSWAIGGGGWSFGWGPQDDEASVA
                     TMRHALESGINWIDTAAVYGLGHSEEVVGRLLKQLPISDRPLVFTKGGLIWDERNRMN
                     PPNRVLKPQSIRLECEASLRRLGVERIDLYQFHWPDETGTRVEDSW"
BASE COUNT         2379 a         3104 c         3190 g         2335 t
ORIGIN      
        1 gacgttgatc atggtttgct gcccgggttc atagacggag acagaatagg tgctcaggtt
       61 aagcgcggca aactggtttc caggttggaa ctgcgggttg ggatagacgg tcagagaggc
      121 gggaagaatt ccgctctggt attgctggct gggcaggccg ttgatccgcg gattggttac
      181 cacgatgtca agcctggcct gtgcgggaaa gttttttgag acggtcactt gcaggctggt
      241 ggaactgttc accgtcgcgg gcagcgccgg aaaggtgccg atccggacag tggcgccact
      301 ggcgaagttg gtgccagtga cagtgagggt gttgtcgaca ttgttggtga tggtggcagg
      361 caacaccctg gtcacggtgg gggcggtagg cggtgaagtc gtgctgatgg tcgcgatgct
      421 gaacccatgc ggcgtgcctc cgccacccag ggttgcgcta cccagaattg ccttgctggg
      481 atcaatcagc atctgggcag tgtcaagctc caccacgttg ggattgggag agtccctacc
      541 agtcttctcc ccaccgacca ccagccggct gccgttgcta ttgatgacgg cggttgacgg
      601 gtataggaaa taggttctca gcgacacctc ggtctggggc agcagttgcc cgaaggttct
      661 ggatccctca ttcaggtcaa ttacagtggc cgagaagcca cttgtgccgt atgccagcaa
      721 atagccgtac accttgccag ccgtgttcac cggagtgatt accagccccg ctggatcgaa
      781 gctgagaggc tggtcctgaa caacctgatc gttggcggtg ttgatgacgt acaggtgaga
      841 gctgccatcc gaattcaaat cctcgaccat cacgacatac ttctgatcgg gtgtcaccgc
      901 agcattcgga gtcgactggc cggaataacc aatgtagccg ctcggcacgt tgacccgcct
      961 gagtctaaaa gttcgaaggt ccagcactgc gatcggggga agggccggat caccagactg
     1021 ggatgtcaca taagcctttt tccccgccac aacaaccgaa cccagggaat aggtgccgga
     1081 gccaagcacc gtggacagat tgactcgccg taccaccctc cccgtggttg cgttgaccat
     1141 ggtcaggccg tagaaatacc cgtaaccgca tccgtcttcc acaaggaggt atttctggtc
     1201 ggaggtgaag gcggcagact ggtaggggca aacaccgtaa atccggttca cctcgcgccc
     1261 gagactgaaa tcaatcacgg ataggtaatc attggctccc acgaagccaa gccgcggatt
     1321 ggcgccaacc agggcaaagc cctgatacgc acccgcagtt atcatttcgt tgatattcag
     1381 ggttgcggga tctaccacgc tgatggtgcc gtacgcgacc ggcgctatga gtttttcctg
     1441 ccccatggcg gcggggctgg ctgacagcag aagcacaagc aagcaaagca cagaaccggc
     1501 tttgctgcag gacaaggacc gaaggagagt tccgtgcata ccgtcacctc caagtatccg
     1561 atcgcgggat ttcaatggcg aaacgacacc ccgacttgcc gccagattgc cgtgagaagc
     1621 tactcatccg cttgggtagc cgcgaaagag caccgattgt gcccgaattc gtcctctggc
     1681 cgcttggacg ggtttagccg agattcccgc agccggaatc ttatatccgt ccactggtgg
     1741 cttgtctgtg atctgtatca cccatcgagt gattccagtt ctggttccca ggcactaaca
     1801 gcaattcacg gcgcgaagta gctggagatg tcgaggatca gttgcgtgag ccccgacgca
     1861 tacgcatcga cctttccgtt agtggtggac acgatcgcca tgttggaggt gatccaaccg
     1921 tcgatcgcgt tcagcgtcga gaattgtaga ggccggccga tgtcggccgg ccatagcgtc
     1981 aggtagccca tcgcgccaga cgggacaacc gtcgcgttga agacatacgc ctgcgctgtc
     2041 gcgggcggtc cacacgggct atccaccaca tccaccggag gactcagggt tccgatgaag
     2101 ggcggaccca gttggcgggt gtcgatgacc cggcagggtg ccccgggata caacgacagt
     2161 cctgtgctcc gcaaaaacct ctgcaggtcg caatctgcat gacttctggt gcagatccgg
     2221 ttgtttcctg cgattgttcc aaagaactct gttcggcatc cgtgctttgt gcgaagctga
     2281 tctcccatgt tgccacttcg aagcgtttgg cagcgtaact tccgccccat ccttactgtc
     2341 ctctgggaac tactgcgcga tgggttgcag ttcctgaacg tcgccacccg ctcgcgaaca
     2401 gccgtagcag cggaaatcct gttcttgcga aagcagttgg cttactacca ggatcaccag
     2461 attcggcctc gccgcctgac cgaggcggca cgattgtcgc tggtactctg gtcccggctt
     2521 ttcgagtgga aagaagcgct catggtcgtt acacctggta cctttgtccg ctggcatcgc
     2581 cgggctttca agctgtattg gcgctggaag tcgcggggcg gtcggccacc gctgcccaaa
     2641 gacatacgcc agctcatcgc tcggatggtg agggagaaca taacttgggg agaagagcgc
     2701 gttgccgacg aactctcgct aaagcttggc attctggttt caccgcggac ggttcgcaag
     2761 tactggccaa agcagccgga cggcaccggg cgcgcccgaa cgtcttcgca gcactggaga
     2821 accttcgtca agaatcatgc acagggcatc atcgcttgtg actttctggt tgcggtcacg
     2881 gccagattcc aggtgctatt tgttttcgtc gtaatggaag ttggcagtcg ccgtattctc
     2941 cactacaacg tgaccgcgca tcccaccgca gactggacgc tgcagcagtt tcgggaagcc
     3001 attcccagct accgacgtta ccagttcttg atccatgatc gcgattcgat tttctcgcgg
     3061 gacctggacg aggagctcaa aggctcgttt gggctgagga tcctgcggac gccggtgcgc
     3121 gcgccaaagg cgaatgcgta ttgcgaacgg ctgatcggaa ccgtgcgtcg tgagtgcctg
     3181 gatttcatga ttccgctgaa cgagcggcat ctgcggcgaa gcctccaagg ctggatcacg
     3241 cactataaca aaggacgccc acactccagt ttggggccag ggtttcccga aaaaactatg
     3301 gatcgacctc tgccacagcc aagaactcac aggcacagac tgccacggga ctgcgagatc
     3361 agagtgatgg acgttctggg cggcttacat cacgaatatt ggctggacaa gatcgttgct
     3421 tgaacgtgca gatgtttctg cggagcacaa cccatttatc gatggcacgc cgtgctttgc
     3481 gcgtatcgac acagccaatc acgagatcga gctctgcgtt cttggcgatc tgctgtattt
     3541 gtgcctgcat gccctgccag ctcagacccc agaagaggtt gatccggtgt gccagggcga
     3601 tggctttcgg aaaaccgatc tccgttcggc agacggcggc ttacagaatc ttgcgggcat
     3661 ccgcagctgg gcttcggcgg tcggtggaca gtcttgtact tcgagtggcg gagatatggt
     3721 gcacgccgga atgttgaagc ctgtacgaac ggttgtgcag tggatttcga gctcacattg
     3781 tagattgctc aattgtcgcg taattgtgct gcggcgaaag taggcgtttt ccaccggagg
     3841 agacaggaag gtagctttgg tcgtttcagc tattagggag aagcaccaag gctgccggta
     3901 gaaaaagcga gggtggcggc agcggatagc gcagagttgt aggcatcatc ggtcgcgaga
     3961 tttgcctgca agagttgagt ctctgcttcg atcacggaag tgatcgaaac aactccttgt
     4021 ttgtaggagt ctagcgctgc atcgtaggtg gtcttggcag cctcctgcaa cacttccgcg
     4081 gcttgattcg cggcaatact ggtacgcagt gcgttctgtg ctgcaacgat ctcctggatg
     4141 gcgtcaatac gaatgcggtc cagagttgcg gcggctttca ccgtgttgtt cttcgcttgt
     4201 tgtattacgt cttctcgacg tttgccgtcg aaaactggga tcgtcaaacc cacgagaacg
     4261 gccgaactcc attgatttcc tgagatgttt agagtcggca gttgatctcc gattgagggg
     4321 agcgcggtta gcccaagttg tccacttgcg tatgcgcccg ttccagcgag gaaaattttg
     4381 gggcggtata gagcctctgc agccttcacc gaagcttggc tggcttgttc tgctgcataa
     4441 gcggcgagaa catcggggcg gcacgccagg gcatctttta ccacctgctc aacaggagtc
     4501 aggatttcgg gaggtaaccg gcgatgttca aacggagcaa ttcggatctt ctccagcggt
     4561 gagagcccca ttgcagcgag cagagtcgca tacatatctc gctccattcc ctcggcctgt
     4621 attgtggcaa gctggatctg cgccctgacc tgacgggcct gggatgactc gagtaaagtt
     4681 ccaacgccac cgctgtaacg agcttcggcc gcattctgaa gctccagcgc atttgcgagc
     4741 gcttgttcgt tggtgcggac acgttgtcgg gcggcgagaa gggcgtaata cgcaatacaa
     4801 acaccatgaa tgactttttg atgcaccccc gtgaatgtga tattcgatgc ctgcgccagc
     4861 ttcttagcag cgctcaccac attggccctg ccgccgaaat cgaacagcag ccattccaac
     4921 gaaattgctg acacagtgcc ggccgcagat ccggtaccgc ttaccgtgaa tctaccggtg
     4981 ccgacgtttc cagttgagtc ctggtatccg ccgacaatgt tcgcggtaac gcgggggaga
     5041 taagtgctct gagcaaaacc cgcggcgagt gcggcatttc gggcaagctg ccaggcaact
     5101 cgggtctcct gatgttcgcg ttcagcgata tcaatcaatt ctggaagcgt gtattcgtgg
     5161 gcgggatcta gtttgaccga cggcttaggt agaacggctg cgtcggggtt gggcgacaac
     5221 aggtagccgt tcttagcgtt cgcgggtgga gtcgatggct gctcgagtac agtcgcgccg
     5281 gacccgtggg acaacccctt gtctggcggt gccgggggcg cggttggctc ttgtgcgcag
     5341 atcggggtaa cgctgatcaa aagcagcagc aggctacgat acatcgagct cttaagcatg
     5401 ttcctccgct tgatgaagcc tgcgcaatgc ctctcgaaac aggttgctcc gagctacaat
     5461 caactccttc aagtgcgccc ctcttcccgt ccgtcgagca agagaaaggt cgcgctgttc
     5521 ctctgattgt ttcccctgga acatctcgat gataccttgc cggcttcggt catcgaatgt
     5581 ctccgaagct gcgagcaggg gcgtctgaag gctctgtgct acatgtacca catcgagtcg
     5641 caagctaagc gacgcgcgtg tgggccgaat cgattccggc tcgtaggcag caagctgaat
     5701 gtcgtcttcg acgccgttga gcaacgtact cgtctccgca aacagtcggt tccgatgcca
     5761 ttggtcagac gatcggacaa ttgcttctag ctgctgagtg gctgtctcaa tagcgcgatc
     5821 gatgcgcggc cccacgctcc tgggccagag ccgagttgcg acaagataag acaagaagtt
     5881 tcccagaagg acgccgatca cgcgatcgcg agcaatcacc atatcgaacg aaggtgacgg
     5941 cccttgcacc acgcagagca ggaaagcgaa ggccatctga aaacccgcat acgagatttg
     6001 cggacttcct actgcgaccc acgctgcaat gaacaccccg ccgaagacaa tcagcgccaa
     6061 atggccaatg cccgttaccc tgggaatgat tcctatcatc acgaagaggc cgaggcctac
     6121 cccgaccagg caaccaagaa tgcgaagggt aagtttttgg acggtttccc caactgtacc
     6181 aagcgacacg atgtagcagg tgatgaatgc agtgtggatt ccgggccagc ccaggagcgt
     6241 ataaatcaga taacacaaca tggcggctgc tgtggttttc accgcaaaat ccacatgttc
     6301 ggggttagtg aaagcatcgg tagcgaggaa ccctgacttt tccttctttc ggggtagttc
     6361 agccggcata attttcccga agtgcttcag cccgacattg aagagcccga tgccagaacg
     6421 cgccaaatcg gattcttcct ctttattgct gatcggttcg acctgggcgg ggtaagcatt
     6481 tcgctcgaag atttgagcca attcgaaaag cttgtgcgtc atctgctctc gtgtcgccct
     6541 tgacggtaaa gcgtcccgct cgctcaccat caactgaacc gcggacagta cggcaacaac
     6601 acaatcggtg gccccctgca atgcatccag atcggcccgt ctgctgctgt gctcgatgac
     6661 agagagctta agctggaact gcaattccgc atccccctcc gcggttagtt tccgtacttg
     6721 ggatatagct tctgtatcgc cctgtagcgc ctggcctgct gtacgcaacc gcagtgccaa
     6781 ctcgcgcaag aggatcttca gaggcgaagg ggcgagaatc aaattgacca cagccgaaac
     6841 gccggcgggg attcccacga agagccaggc gtaaagtaat gcccttgtgg ccagttcccc
     6901 aaacggagca gagccaagca cgtcgagtgc gtacgctatg accaatgcaa catcagaccc
     6961 aacgggcttc aacttgcttg ccgagactag aaataataag caaaacgaca cccctgccat
     7021 cgtcccaacc cggagagagg gattcgcaag aactggcgac gcaatgagaa acaggaaaga
     7081 caaaacgatg gtggcaaaga tcgtcagggc gatcgtaaga gccatactgg atacccggtc
     7141 ggctttgttc aggaagaagg ctatatagat ggtcagagcg atttccgggg tctggtagac
     7201 ttccgcgacg acagcggtga gcgcacagat gagggccaac cgagccgcga aggcaaatct
     7261 ccccggcgat cgttccgtca gaagccgcca gaggcggatt cctgaacctt cctcatcggc
     7321 aagcgacgcc atgatccacc tccacgatgg cgctggctcc aacgcgcacg agggtctgcg
     7381 gggggttttc aagcctgatt cgcactggga atcgctgggc gacgtggacc caattcagcg
     7441 aaggctgaac gtatggcatg aagcgcggca ggttaatgcg atcgtcggac aaaacgccaa
     7501 agcccacgct ctgtatgatt cccttcatcg gaacccgacg gttgatcatg gagtaagccg
     7561 ttacgcagtc ccctggtttg acaaggtgca gatcaaactc gcggaaattg gcggagacaa
     7621 accactcctc agtattaatc aacgtgaaga tcgattgaga aggcacaacg atctcgccgg
     7681 tcgatatgtt caagccaaca acgcgtccat cgtgcggggc tcgtaccgcc gtgtcttcca
     7741 atgctctgcg agcattggcc agcgttgcgg ctgctgcgcg tacgcctgca tccgccgcat
     7801 caatattgtc gatcaagccg tgggcggcct gttcctgctt ctctgactcg ctaagggaaa
     7861 tacccgcatc ctgcccggca acctctgctt gatcgagctg ctgcctgggc acataaccct
     7921 ttgcggtaag aggcttcaga cgctcttccg tgcggagtgt cagagcaaga ttggattgcg
     7981 cacggcgaat ttggtcgctg gcgatctccg cattggaacg ctccgtcgaa acgagtcgcc
     8041 gcttggatcc gagtgcagca cgtgcaagat caaactgtgc cctggctgcc gtgacagtgt
     8101 tttcataagg ggtgggatca atcctgaaca gaaggtcgcc cttgtgcaca tattcgttct
     8161 cgtgtacggg cagttcaatg atccgtccac cgaccggcga cgccacgtgc accacttccg
     8221 catcgatcga ggcatcgtcc gtggacagtg tggttcgcga ttcaacccaa aaccaaagcg
     8281 cgattgctgt accaacgatt actgtggcta gaactgcacc gagcggcttc cgtttgcgaa
     8341 tcccgtgggc ttccgtcatt aagacactac ccatgtgaac caaacgataa gcccggcaat
     8401 tgtcccaatt gaggcgcaaa ctgcgagctg gaggggaatg agctgcgaga ggcgggtagc
     8461 gaccatgatt acgcgcgcta cgcacgccac gaggaccgcc agagtcgcgc aggcaagcca
     8521 tgccggaaag tatgcgccgg ccaccgttaa tgaaggtgct ccgcacccgc aaagcaacag
     8581 tgtgtaccca aacaacagca cggatggcac ggtaagcctc gcgccagggt gacatgaatc
     8641 tgagagcatg tatcgaattg acctgaaagt gctaacaggg gcaggctcga atgaagttct
     8701 cccgcatgct tccagcttct tttgcatggg taatgcccat gagtttcgtg tagaaatgtt
     8761 tctcaactta tgacccaatt gcgtcaaaaa tcgcgtcgtc aaattgttta acccgtttcg
     8821 ctttggtcgg gtcgaatctt gaggctacga gacagttcgt ctcacaagtg acgcgctcag
     8881 agaaacgagc atttgtcttt gctttcgctc agttagacga tctcatgccg caggcaggaa
     8941 agcgtccctg ttatagcggc atgcactgta gtgtctttgc cgtcatactc gcaattgagc
     9001 cacagtgcct gcccctacga ctgtgactgc tatcaccccg tcatgtgctg tgaatacctc
     9061 tcggttatgg cagcgttgcc ctacagccgc agtggcgtga tatcgcgccc atcattccga
     9121 tctctggacg gttcaccttt gcttggacca ccggcgggac gatcggtcgt ggcagaagtc
     9181 gatccatcgc tctggaaccg gggcttcgcg ccggagaatt ggaacgaaca ttagcagggc
     9241 gcattttccg ttgccgaatg gatgcgccgc tctgaccttg acggcacact cgcggatttc
     9301 ttggctccct ctctcgtgcc tgcagagcgc gaaacagcgc acattgaggg ctggatactc
     9361 ggaattaaat gaacgccgct acgctattcc gctattgctg ggagaatgcg gcgctgaatc
     9421 cttcgcggcg gcagtatcct tattactggc ggaggcaccg atggttgaag tgcacatgca
     9481 ctggtcacaa gcggtttacg ccgttcagcc ctcataggag aaacaatgga ctacgtcaat
     9541 ctcggcaaga ccggccttaa ggtttcccgc atctgtctcg gctgtatgac ttacggcgtg
     9601 cctgctacgg gcgccctcag acccggcagc cacgcttggg cccttaacga agaggaaagc
     9661 cagccttttt ttcgccaggc gcttgatctc ggaatcaact tcttcgacac cgccaacgtc
     9721 tactcaggcg gatccagcga ggaggtgctg gggaaattcc tgaaggcaaa tacgcgtcgg
     9781 gaagccgtca ttatcgccac caaggtacac ggcgtcatgc gcgacgaacc caatggccgg
     9841 ggcctctccc ggaaatccat tttctacgag ctggaccaga gcctccgccg gctcggaacc
     9901 gactacgttg acctctatca gattcatcgc tgggattacg aaacgccgat cgaggagacc
     9961 ctggaggtct tgcacgacgt ggtgaaatca ggcaaggtgc gctacatcgg cgcctcgtcc
    10021 atgtttgcgt ggcagttcac caaggcgctc tatcttgccg accttcacgg atggattcgt
    10081 tttgtctcca tgcagaatca ctacaatctg ctctatcgcg aagaagagcg cgagatgatg
    10141 gccctttgcc ggtcagagga catcggcgtc atcccttgga gccctctcgc gcgcggtcgc
    10201 ctgacccgcc cctggcaaag cgaaaccacc aaacgctatg aaaccgacca gttcggcaag
    10261 aagatgtacc tgcagacgga agaggccgac cgtaaagttg tggaccaatt gggacagatt
    10321 gccgaacggc gtggcgttcc ccgcgcccag ctcgcgctgg cgtggctgct cagcaagccg
    10381 ggggtcaccg caccgattgt cggagccact aaagcgcaac atctccagga cgcagtagca
    10441 gcgctctcgc tgcatctgac gcccgaagag attgcttctc tcgaggaacc gtacacgcca
    10501 catcccgtcc ttggcttcag ttgagtcggc gtgatttgtt ttgccttcag aaggaggaaa
    10561 cacatgcaat tatccaagcg gccgctcggt tctagcggcg tcatgatcac cagcgtcggt
    10621 ttcggctcat gggcgattgg cggaggcggg tggtccttcg gatggggtcc gcaggacgat
    10681 gaagcgtccg tcgcaaccat gcgacatgct ctcgaatcgg gtatcaactg gatcgacacg
    10741 gccgcagtct acggcttagg ccactccgaa gaagtcgttg gcagactgct aaagcagctt
    10801 cccatctcag accggccgct ggtgttcacc aaaggcgggc tgatctggga cgagcgaaac
    10861 cgtatgaacc cgccgaatcg tgtgctgaaa ccgcaatcga tccgcctgga atgcgaggcg
    10921 tcgctgcgcc ggcttggcgt cgagcgcatc gacctctacc aattccactg gcccgatgaa
    10981 accggcaccc gcgtcgagga ctcatggg
//
LOCUS       DASRKK010000338        23289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1101490, whole genome shotgun sequence.
ACCESSION   DASRKK010000338 DASRKK010000000
VERSION     DASRKK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 23289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101490"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="pth"
                     /locus_tag="VFC15_07305"
     CDS             complement(<1..419)
                     /gene="pth"
                     /locus_tag="VFC15_07305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZM10001.1"
                     /translation="MKLIVGLGNPGVEYQFTPHNMGFLAVDRIAEQCGVRVNNRNCRA
                     QTARARIGETEVVLAKPETFMNLSGLSVRELVAEYQVQPEQDLVLLYDELDLPFGTMR
                     IRQRGRSAGHNGVESVIGALDSQEFVRIRMGVGPDHPV"
     gene            complement(425..1171)
                     /locus_tag="VFC15_07310"
     CDS             complement(425..1171)
                     /locus_tag="VFC15_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZM10002.1"
                     /translation="MATTTPEQNVETKPRQSGTKNDARRIRKSGLVPAVLYGAGLAPL
                     NLSVDPKQMKRILNSESGHNTIFDLSAGGDSGKAMIVDWQFEPIKGALLHVDLKRIAM
                     DKLLRVSVPVTLKGEAHGVKVQGGILEQMVREVEIECLPADIPGRIEADVSKLEFGQT
                     IRVADLPRNEKIKFITDENQAIAHVTAVKEVVVETPVEGVEAAATTAEPEVIKKGKAE
                     TEGDAAGGEKAEKGAKSDKGDKAEKSEKKK"
     gene            complement(1185..2261)
                     /locus_tag="VFC15_07315"
     CDS             complement(1185..2261)
                     /locus_tag="VFC15_07315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZM10003.1"
                     /translation="MEAAGVKPGVAEQAAASETATAVAQQSDKKQQTAADPKAKPVRA
                     RIEDKWKIFSGTANDALAKEVCDKLGLPLGQTRITKFSDGETYVQILENVRGSDVFLV
                     QPTCDPVNEHLLQLLLLIDALKRASARRITVVVPYFGYARQDRKDRPRVPISSKLVAD
                     LLTTAGADRALVVDLHAPQIQGFFNIPVDHLFASPVLVGFFRDLQLPDLTVVSPDAGG
                     VERARFFAKKMDSALAIVDKRRVDLDVTEVMHVIGDVKDRTCLILDDIIDTAGTLVKT
                     CQALLDAGARQVYACATHPVLSGPAIERISKSGLVEVVVTNTIPLTEAGRNEPKIRQL
                     SIAGLIASAIQSIHEETSVSRLFL"
     gene            complement(2449..2522)
                     /locus_tag="VFC15_07320"
     tRNA            complement(2449..2522)
                     /locus_tag="VFC15_07320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2488..2490),aa:Gln,seq:ttg)
     gene            complement(2591..3610)
                     /locus_tag="VFC15_07325"
     CDS             complement(2591..3610)
                     /locus_tag="VFC15_07325"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10004.1"
                     /translation="MSTSISSFAKINLGLKIGAARADGFHELRTIYQTISLHDVVRVE
                     VQPGDGIEIRCEDSRVPCDATNTCYKVAERVMRAVEASGRVVITIDKRLPVQGGMGAA
                     SSNAVATMIALERELQTDIAPQEKFRIAAEVGSDLPLFLIGGTVLGVDRGQEVYALQD
                     LPPMHLVVVTPPVGVSTPNAFAQWDALPAHESLLNAAGEPALTTLSAAGTITAFSQSV
                     FAWLEKVFPLQAQPASGVPASGGDRAETPLLDLVRTGIENDFERVVFPEYPELREVKR
                     VLEREGAQYTSLSGSGSTLYGVFASAAEAESAAGRMSDAGHAAVATTTLDRAEYWEQL
                     VTGNW"
     gene            complement(3759..3887)
                     /locus_tag="VFC15_07330"
     CDS             complement(3759..3887)
                     /locus_tag="VFC15_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10005.1"
                     /translation="MGALATSPDSSHSWGLALGMTAALVYMPFAQRSLVLMYARNR"
     gene            4192..5334
                     /locus_tag="VFC15_07335"
     CDS             4192..5334
                     /locus_tag="VFC15_07335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM10006.1"
                     /translation="MPFEPGHILGDYEVLGPLGSGGIGEVYKVRHVISQRTEAMKLLR
                     ADRTGGELPQRFLREIRVLASLSHPSIARLHTAFNVDDQIAMVMEFIEGEDLHWKLHR
                     QWPGRAVEGIEYICQVLSALEYAHARDVIHRDIKPSNIMITPERQAKLLDFGMAFKAA
                     DLSVTRPGNILGSIHYMSPEQVRGERVDARSDLYSTGVTLYEIITGYRPFDGKTEYEV
                     MTGHLREQAKWPGGIDPAIPYGLSTIVMKALAKNPDDRYQNAADFLVALTHISLDDAA
                     TLHTLAIQPSASSSPSDGVKPPSGSPVSKPGSATPKPSNNALDPSILDSVSRELAAFI
                     GPIAKVVVKRAAGRCSSVDELYSAVAAEIDSDKDRSSFMASKKRRV"
     gene            complement(5349..6224)
                     /locus_tag="VFC15_07340"
     CDS             complement(5349..6224)
                     /locus_tag="VFC15_07340"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HZM10007.1"
                     /translation="MKLLTKFNLVLIVFFGAGGFIIAHLAYSFLTQNAREQVLQQAEL
                     MMASARATRDYTSEQIKPLLLKNPEHETNFLPQTVPAFSATTIFNSIRKTYPNYAYRE
                     ATLNPTNLVDRADDWETDIIRYFADHPGDKKLVGERDTPAGRQLYLAKPIAVAASCLE
                     CHSTPDVAPAAMIRKYGSANGFGWTPNQIVTAQIVSVPMSVPLGVADDAYRRLLFFLI
                     ATFVVTIVALDAALYLLVIRPLHKVSSAANQISKGDTDLPELTVKGKDEIADVTASFN
                     RMHVSLAKALKMLDQ"
     gene            6378..7139
                     /locus_tag="VFC15_07345"
     CDS             6378..7139
                     /locus_tag="VFC15_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10008.1"
                     /translation="MKYLITPGRFFFAIAITAFGIQHFIYAITGGGLGPPWTPANHLL
                     AYLVGAALIAAGIGLATGIQVRCAGIMLTSVVVIRALLCYAPKLAANVRDPGPWTSAF
                     EILAMGGASLVLAATFTPRRSRTDRTGARPGALFQAGRFLFAISLIVFGIQHFLYATF
                     IATLITPWIPGHLFWAYFVGVAFIASALAIASGIVAPLAATLLGTMFFLWVVVLHAPR
                     VAAAVQNANEWTSLLVALAMSGGAFIVAGALADRD"
     gene            complement(7284..8048)
                     /locus_tag="VFC15_07350"
     CDS             complement(7284..8048)
                     /locus_tag="VFC15_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZM10009.1"
                     /translation="MPQLMRTSNPALGQDTFRTVESTLGEGMTISGAVNKTGILVLLC
                     VASAAFTWNRFFAATSPEEAMAGMAPLVAIGGIGGFIVAMVTIFKKQWSPITAPIYAL
                     LEGLLLGGVSAMFEIRYHGIAIQAVSLTFGTLIVMLLAYRSGLIKVTDNLRLGIVAAT
                     GGIAVFYLIQFVLGFFGFRFNAVNGSGPIGIIFSLVVVGVAAMNLVLDFDLIEQGARF
                     NAPKYMEWYSGFALMITLVWLYFEILRLLTKFRSRN"
     gene            complement(8144..9235)
                     /gene="ruvB"
                     /locus_tag="VFC15_07355"
     CDS             complement(8144..9235)
                     /gene="ruvB"
                     /locus_tag="VFC15_07355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZM10010.1"
                     /translation="MRPPPLSLYHRPIVVFPKDSLDRERIVSAAPVDDDASFELKLRP
                     QRLREFIGQDKVKNNLEIAIQAAKSRGEALDHVLLYGPPGLGKTTLANIIANELGAAF
                     QQTSGPTLQIKGDLTAVLTNLRDKQVLFIDEVHRIQPVLEELLYSALEDYKLDIIIGQ
                     GPSARTHTIEVRPFTFIGATTRAGLLSAPLRSRFGIVLRLEFYTHADLKIILERSAEI
                     LGVEIDEPAAIEIATRCRGTPRIANRLLRRVRDYAQVRGNGKIDKVTAQSALEMLEVD
                     RYGFDEIDRKLLLTIIEKYQGGPVGVNTLAASLAEEADAIEEIYEPFLIQIGFLDRTP
                     RGRVATHLAYQHFGIKRPKTNAAGQETLF"
     gene            9655..11052
                     /locus_tag="VFC15_07360"
     CDS             9655..11052
                     /locus_tag="VFC15_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM10011.1"
                     /translation="MAEQLITTDARPVRLRRELGLGSAAAAVAGEAIAVGIFLTPAGM
                     AKLLGSPFWLLFVWLLMGAISISGALCFGELAARYPEAGGLYVYLREAFGERVAFLYG
                     WMSMLVMDPGVSAALAVGMATYASFIFGWSTTVAKLFAIASIAAICGLNMINTRASAG
                     FLRYITWLKFAILALLVIWALVFRLGSWSHFVPFVAQRSGSTPLLPALGGALVAAFYS
                     FGGWWDVSKIAGEVKDPARTLPRALVLGVVGVVAAYVLVSGVFLYLVPLGSVTNDQTF
                     VAQAGAILFGRTGANLLAGAVIVCVLGSLAALFMLCPRVYYAMARHGVFIHSVAELHP
                     VFATPMRAIGIQGIMASLLVALGAFDQIIAYFIFVAVLFIGLTVSTVFVFRRRERGAA
                     PTIRTPGYPFTPLAFLGMVLLLLTLMMLRSPRQALLGCAVVLLGIPVYALIQRRREAM
                     SATPEAILNHALDRQ"
     gene            11049..11378
                     /locus_tag="VFC15_07365"
     CDS             11049..11378
                     /locus_tag="VFC15_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10012.1"
                     /translation="MSDETKLAAQTNEPVIRPAVAGEALPPTKIRITEDAEAIGDTLA
                     AYDYWRAGSGRTQVPGIIKCFGSRPDFLRQVVDFSNTVHFSEGHLVRRHKEMIASYVS
                     YLNRCPY"
     gene            11493..11696
                     /locus_tag="VFC15_07370"
     CDS             11493..11696
                     /locus_tag="VFC15_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10013.1"
                     /translation="MDYAKIVTEAAYRSTPQDVQKLRDHGWSEDQISEAVYIIAMFAF
                     FNRVADAFGVPAQHYLELGKMTP"
     gene            11727..12344
                     /gene="purN"
                     /locus_tag="VFC15_07375"
     CDS             11727..12344
                     /gene="purN"
                     /locus_tag="VFC15_07375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZM10014.1"
                     /translation="MIAPSMKNLGILLSGRGSNFEAIAGNVASGKLDANLAVVISNRA
                     DATGIESARRRGLKALVIPSKGKPREEHDREMVTTLRDHHVDLVCLAGYMRLLSPWFV
                     QQFPNRILNIHPSLLPAFPGLDAQKQAFDYGVKVSGCTVHFVDADLDHGAIILQKVVP
                     VLDTDDDHTLAARILEQEHIAYSEAIRIVLGGQYTITERRVVMLT"
     gene            12367..12675
                     /locus_tag="VFC15_07380"
     CDS             12367..12675
                     /locus_tag="VFC15_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZM10015.1"
                     /translation="MSKGVVVKRTKKKVARRPPGQRQMAAGEFKAKCLGVLDEVQDTG
                     RHIVITKRGKPVAKVVPITDGKRKDNFLGRLEGIMKINGDPDDLVNPIFPLEDWDMLK
                     "
     gene            12672..13055
                     /locus_tag="VFC15_07385"
     CDS             12672..13055
                     /locus_tag="VFC15_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM10016.1"
                     /translation="MILLDTHVLVWANLATRKLSRPAESAIRRARGSVAISAISLLEL
                     ARLISANRIDRRGSVRETIDSLTQGIEIRPITAEIAALTAYLPADFPSDPSDRAIAAT
                     AQAEGIPLVTADERIQACSLIRIIW"
     gene            complement(13074..13616)
                     /locus_tag="VFC15_07390"
     CDS             complement(13074..13616)
                     /locus_tag="VFC15_07390"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZM10017.1"
                     /translation="MSAKPQNCDDLKTLIREVPDFPKPGILFYDITTLLKDRLGFARL
                     IDSLTENYIDKDIDLVLGIEARGFIFGPALAYRLNAGFVPVRKPKKLPAETLKWTYDL
                     EYGSDTLEIHKDAITPGQRVLICDDLLATGGTARAAAEMVKSMGGDILGLSFVVELDG
                     LKGRDKLKEYDVFSLLHYEM"
     gene            complement(13621..13905)
                     /locus_tag="VFC15_07395"
     CDS             complement(13621..13905)
                     /locus_tag="VFC15_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZM10018.1"
                     /translation="MPDIETRRFVVTGRVQGVGFRWFVEREAAQLNITGWVRNRENGS
                     VEVMATGTRDQLVALHGRLREGPRAARVDDVAASPAPFLDAKSFGIEGVW"
     gene            complement(14152..14838)
                     /locus_tag="VFC15_07400"
     CDS             complement(14152..14838)
                     /locus_tag="VFC15_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM10019.1"
                     /translation="MKRWFAVVLCTCLALPLIAQEKETSRLKESYDVLKAVAGMPDKG
                     IPRDLLNKAECVIVVPAVKKAAFIVGASYGRGVMTCRSGPEFRGLWTAPAFYAIEGGS
                     FGLQAGGQATDYVLLIMNEQGAKSVLTSKVKLGGDASVAAGPVGRATSAETDIVMKAE
                     ILSWSRNRGVFAGISLAGSTLRSDDDANKNLYGKELNATQIVRENAVKTPAAGKPLVS
                     YLDKISPKHM"
     gene            complement(15119..15793)
                     /locus_tag="VFC15_07405"
     CDS             complement(15119..15793)
                     /locus_tag="VFC15_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM10020.1"
                     /translation="MKKLLLVVLCLIVTLPALAQSKVDKRLSESTAVLNTLLSKPDGI
                     NKTLLEKAECILVYPAVRKVAVGIGGTYGRGVLVCRTGKAMNGHWGSPIMYTLDTGSL
                     GVQLGTSSTDYVMLVMTQRGADKILSGKLKLGADAQAAAGPSAAQANGWNDPNVDVLT
                     YSEAKGLFAGAALGSASMAADDDANEKLYGKSIDAMEIVRGDAAVPAPGKPLVDLLNK
                     TAEKHM"
     gene            complement(15978..16538)
                     /gene="efp"
                     /locus_tag="VFC15_07410"
     CDS             complement(15978..16538)
                     /gene="efp"
                     /locus_tag="VFC15_07410"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZM10021.1"
                     /translation="MAIPATQMRPGMVIKHNNDLHTVFSVEHRTPGNLRAFIQAKLRN
                     LRTGAMFEHRFRSGDAIDKITVDQVQMEYLYQDGENHVFMNTENYEQLHLGKDVLGDA
                     VEYLTANLQISVEFFDDKPVGVELPQTVELTIVETEPGLKSATASSVTKPAKTETGLV
                     VYVPPFINEGEKIRVDTSDGAYLSRA"
     gene            16701..17024
                     /locus_tag="VFC15_07415"
     CDS             16701..17024
                     /locus_tag="VFC15_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZM10022.1"
                     /translation="MSDEIEDVVSEGEYRPSGSNTLVIGLALLVVGFAAGAIAAALMT
                     PKTGKQMRRELKRKADGLRDMVDDLSDQASDWVDKGSDLAGKASDWADAAKEKVAPLA
                     KKFRS"
     gene            17429..20257
                     /locus_tag="VFC15_07420"
     CDS             17429..20257
                     /locus_tag="VFC15_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HZM10023.1"
                     /translation="MRKKAPFLPGRAAFICAVMFLSLTIAGQAQQPLQVLHNHVRPAV
                     SSGQAVPVGLLPSSQRLNLAIMLPVRDQGELTRFLDRLYDPSSPDYHQFLTVAQFTEQ
                     FGPTHQDYQAVIAFAKANGLTVTDTPANRLLVDVNGSVAQIEKTFHVGMKVYLHPTEN
                     RTFYSPDREPSLDLSVPVSHIAGLNNFSIPRSMLKKGSAAQQNLTSNVGSGPGGAYLG
                     SDMRAAYYGGTALTGTGQAVAMVEFDGYNMSDVTGTFDGHTYTVPINNVLIDGASGGS
                     DGDDGEQVLDIVQAISMAPGLSQLRVYIAPITTSIGVGDVDMFNKIATENIAKSISCS
                     WGWNPADTAANDPIFQEYAAQGQNLFVASGDSGAYTGSNATDSSFPAEDVYVVAVGAT
                     DLTTNGAGGPWQSEVAWADSGGGPADNGFAIPSWQIGVADTANGGSNTIRNVPDVAAE
                     GNFDNYLCDQGSCQGGWGGTSFAAPRWAGFLALVNQQAVTNTGSTLGFVNPAIYAIGK
                     STNYNSDFHDITSGNNNNGKGKSFNAVVGYDLVTGWGSPNGPSLINALAGATSPSFTL
                     SDSPSSLTITQGAAGGTTTVTVTDLNGFNGSVSLSASGLPSGVTAAFNPTSTTTTSVL
                     TLTASRTANLGTVTVTITGTSGSLVASTTLTLTVQAQAVPNFTIGASPNSLTVAPGGN
                     GTSTITITSQNGFSSATTLTATGLPSGVTAAFAPNPVTPPANGPITSVLTFTATSTAT
                     VGPATVTVTGTSGSLVHSTTIALTVGTSTAQTAVYNATLKAPGCATVGISCDSGPSLL
                     LGRDHLSGGAEPNQPNTINNSCADGTTGTFHSDESNDRILVASTSGSALTHGNTATVT
                     ATVWAWSGFSSDSVDLYYAANANSPSWVLIKTIKPTKAGAQSVSATFTLSTGALQAVR
                     AQIRYQGTASACTLGSYNDHDDLIFAVQ"
     gene            complement(20437..20757)
                     /locus_tag="VFC15_07425"
     CDS             complement(20437..20757)
                     /locus_tag="VFC15_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HZM10024.1"
                     /translation="MLQRKISLVLALGLLALPVMAQDTKQPSAAQAAQQQRMKDCNAQ
                     AKAKNLAGDERKTFMSSCLSAAGVPQTQQDKMKSCNKEATAKNLKGDDRKSFMSTCLS
                     GSTK"
     gene            complement(20883..21779)
                     /locus_tag="VFC15_07430"
     CDS             complement(20883..21779)
                     /locus_tag="VFC15_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZM10025.1"
                     /translation="MSTPVLTKAETEKPPTVEQKHDILPTLFGEGEGLYPQRKDTFVY
                     SFILHMIAAGLLIWSGHWVVTHQDEIKQQVVGLVTDISPYLPLPVSKTRAGGGGGGGD
                     RDKLQAPKGALPKLSKDQIVPPAIVVRNENPKLVVTPSVVVPPEIKLPTVGALGDPLS
                     KVLGPPSNGTGYGGGIGSGSGGGVGSGTGPGVGPGHGGGYGGGAYRVGGGVSAPKALY
                     APDPEYSEEARKAKYQGTVVLWLVVSADGRPQQIRIQRALGMGLDEKAIEAVKLWKFE
                     PARKDGQAVPVMINVEVNFRLY"
     gene            complement(22243..23055)
                     /locus_tag="VFC15_07435"
     CDS             complement(22243..23055)
                     /locus_tag="VFC15_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10026.1"
                     /translation="MEYRTCTYIHDDGGICNSAAVTDRNLCLYHLRHRARLMRMAQYR
                     ARGEHFDLILPPLESTFGVQSALTQLSEALAADMIELKRADRLIRVLNIASRNLLKSD
                     KWPASPYHSDQPAPAVDLTADFGLPNDLDLNLTPELAFPPPPGIAFSDSSSRAQAAAS
                     ADGVEGPASSLSPMPTVAYCKHGPGCSEHTIRADYPETAELAELREIQRTQGDEAMGL
                     RHKQQQRNRRRRHIVTNRKRYAAIALEKNMRLAAERLAEQKLAGKGTCRRQP"
BASE COUNT         4597 a         7030 c         6806 g         4856 t
ORIGIN      
        1 accggatggt ccggcccaac tcccatgcgg atgcgcacaa actcctgcga atcgagggcg
       61 ccgatcaccg actccactcc gttatgtccc gccgaccggc cccgctgccg aatccgcatc
      121 gtgccgaacg gcagatcgag ctcgtcgtag agcagtacca ggtcctgttc gggttgcacc
      181 tggtactccg ctaccagttc gcgcaccgac aggccgctca agttcatgaa ggtttccggc
      241 ttggccagca ccacttcggt ttcgccgatg cgggcccgcg cggtctgcgc gcgacagttg
      301 cggttgttca cccgcacgcc gcactgctcg gctatacggt ccaccgccag aaaccccatg
      361 ttgtgcggtg tgaactggta ttcgacgccc ggattgccga gcccgacgat cagtttcacg
      421 ccgcctactt cttcttctcg gacttctctg ccttgtcgcc cttgtctgac ttggcgccct
      481 tttcggcttt ctcgccgccg gcagcatcgc cttcggtctc cgctttgccc ttcttgatga
      541 cctcgggctc ggcagtcgtc gctgcggcct cgactccctc gaccggagtc tccaccacaa
      601 cttccttcac cgcggtcacg tgggcaatgg cctggttctc gtcggtgatg aacttgatct
      661 tttcgttgcg cggcaaatcg gccacgcgaa tggtctgacc gaattccagc ttgctgacat
      721 cggcctcgat gcgtcccgga atgtcggcgg gcaagcactc gatttcgact tcgcgcacca
      781 tctgctcgag gatgccgccc tgcaccttca cgccatgcgc ctcgcccttc agcgtgaccg
      841 gcacactgac gcgcagcagt ttgtccatgg caatgcgctt gaggtcgacg tgcagcagcg
      901 cgcccttgat gggctcgaac tgccagtcca cgatcatcgc tttgcccgag tcgccgcccg
      961 ccgagagatc gaaaatcgtg ttgtgacccg actccgaatt caggatgcgc ttcatctgtt
     1021 tcggatcgac ggagaggttc agcggtgcca gtccggcgcc gtacagcacg gccgggacga
     1081 gcccgctctt gcggatgcgg cgcgcgtcat tcttggtgcc gctctgccgc ggcttcgttt
     1141 ccacattctg ttcaggagtt gtcgtagcca taatttcttg tcctttacaa aaacagtctg
     1201 ctgacggatg tctcttcgtg aatcgactga atggcgctgg cgatcaagcc ggcaatcgac
     1261 agttgccgaa tcttgggttc gttgcgcccc gcttccgtca gcgggatggt gttggtcaca
     1321 accacttcga ccaaccccga ttttgaaatc cgctcgatgg ccggacccga aagcacagga
     1381 tgcgtcgcgc aggcatacac ctgtctggca cccgcgtcga gcaacgcctg gcaagtcttc
     1441 accagggttc cggcggtatc gataatgtcg tcgaggatga gacacgtgcg atctttcacg
     1501 tcaccgatga cgtgcatgac ctcggtcaca tccaggtcca ccctgcgctt gtcgacgata
     1561 gccagcgccg aatccatttt cttggcgaag aaacgcgcgc gctccacgcc gccagcatcc
     1621 ggagaaacca cggtcaggtc cggaagctgc aagtccctga aaaaacctac cagcaccggc
     1681 gatgcgaaca agtggtccac cggaatattg aagaaccctt ggatctgcgg tgcgtgcaga
     1741 tcaaccacca gcgcccggtc cgcccctgcc gtcgtcagca ggtcagccac cagcttcgaa
     1801 ctgatcggta cacgaggccg gtccttgcgg tcctgccggg cgtagccgaa atacggaacc
     1861 accaccgtga tgcggcgcgc cgaagcgcgc ttcaaggcat cgatcaacag caatagttgc
     1921 agcaagtgct cgttcaccgg atcgcacgtc ggctgaacca ggaacacgtc gctaccacgc
     1981 acgttctcca ggatctgcac atacgtctcg ccgtccgaga atttggtgat gcgcgtctgt
     2041 cctagtggca ggccaagctt gtcgcagact tccttggcca gcgcgtcatt cgctgtgccc
     2101 gagaagatct tccacttgtc ctcgatgcgc gcgcgcactg gcttagcctt cgggtccgca
     2161 gccgtttgct gtttcttgtc ggactgctgc gccacggccg ttgcagtttc gctggccgct
     2221 gcctgttccg ctacgccggg ttttacgcct gctgcttcca taagtttgta ccccgccaaa
     2281 cctggtttga ttacaagctg ggtcgaaact acgaactaat cggtgactgg tcactggtaa
     2341 ttagcaagaa acgctgatcg gatcactcgt tgctaactac gccttcactt ccgaacaact
     2401 gattacacct tgctgacaac tgactactgg taatcggcaa ctgaatcttg gctgggcggc
     2461 taggattcga acctagacaa gtgcctccaa agggcactgt cctaccatta gacgaccgcc
     2521 caatcggtaa ctggtcactg gtaattggta gctggtaaaa caaccggagc gacttcgtgc
     2581 gctgggccag ttaccagtta ccagttacca gttgctccca atactccgcc ctgtccagcg
     2641 tcgtcgtcgc cactgccgca tgtcccgcgt cgctcatgcg ccctgcggct gactctgctt
     2701 ccgcggccga cgcgaacact ccatacagcg tcgatccgga accagacaac gacgtatact
     2761 gcgcgccttc gcgctcaagc acacgtttga cctctcgaag ttcgggatac tctgggaaga
     2821 cgactcgttc aaagtcgttt tcgatcccgg ttcggacgag gtcgagaagc ggcgtctcgg
     2881 cccggtcccc gccactcgcg ggaacaccgg atgcaggctg ggcctgcaag gggaaaactt
     2941 tctccagcca ggcaaacaca ctttgactga atgctgtaat tgtacctgcc gcgctgagag
     3001 tggtcaatgc aggttcgccc gctgcattta gaagcgattc gtgtgcaggc agcgcgtccc
     3061 attgcgcgaa agcattgggc gtcgaaaccc ccaccggcgg cgtcaccacc acgaggtgca
     3121 tcggcggaag gtcttgcagc gcatacactt cctgaccgcg atccacaccc agcactgtcc
     3181 cgccaatgag gaatagcggc agatcgctgc cgacttcggc agcgatgcga aatttctcct
     3241 ggggagcgat gtcagtctga agctctcgct ctaacgcgat catcgtcgcg accgcattgg
     3301 acgaagccgc gcccatccca ccttgcaccg gcaaccgctt gtcgatcgtg atcaccacgc
     3361 gaccgctggc ttccaccgcg cgcatcacgc gctccgccac cttgtagcaa gtattggtcg
     3421 cgtcgcacgg cacgcgagaa tcttcgcagc gaatctcgat cccatcgccg ggttgcactt
     3481 cgacgcgcac cacgtcatgt agcgagatgg tctggtagat agtacgcagc tcgtgaaagc
     3541 cgtcagcgcg cgccgcgccg atcttcagcc cgagattgat cttcgcgaat gagctaatcg
     3601 aagtggacat gggggaccag tggctagtgg tcagtggcta gtgtaaagca ggggtcagtt
     3661 tcaagtttcg agtcgccatt ttaccctgag ggcggggacg gtagcacaga aagcaaccct
     3721 ctgcaagaaa aactagatag aaaatctggg caagtagctt accggttccg cgcgtacatc
     3781 aagaccagcg aacgctgcgc gaacggcatg tacaccagcg cggcggtcat ccccagcgcc
     3841 aagccccagg aatgtgatga atcaggagaa gtggcgagag cgcccacaac aaatccatcc
     3901 agagcagcac gggtaaagat gcgtttgaac ttttcttgga gttactgaaa gtcgaacact
     3961 accgcggtga aaagaaacag ccggaccggg atcagcaagc aggtagcctc acgatggcct
     4021 aatgcgcatt gtgcaccccg tccgtgactc tggcaaccag gtatcgccag tcagccacct
     4081 gggggcctac atcgctcgct tgcaatccgt gacctcgcca cgcaaaatgg agcgggatgt
     4141 ttctgacaat ttccaatccg accgcggcag cgagccgttc ttgggacagc tttgccattt
     4201 gaaccgggcc acatcctcgg tgattacgaa gtgctcggcc cgcttggttc tggcggcatc
     4261 ggtgaggtgt acaaggtgcg gcacgtcatc tcgcagcgca ccgaagcgat gaagttactg
     4321 cgcgccgacc gcaccggagg cgagctgcca caacgttttc tgcgcgagat ccgcgtgttg
     4381 gccagcctgt cgcatccttc catcgcgcgc ctgcataccg cctttaacgt cgatgaccag
     4441 attgccatgg tgatggagtt catcgaagga gaggacctgc actggaagct gcatcgacag
     4501 tggccgggcc gggcggtcga gggaattgaa tacatttgcc aggtattgtc ggcgctggaa
     4561 tatgcccacg cgcgcgatgt gattcaccgc gacatcaagc catcgaacat catgatcacg
     4621 cccgaacgcc aggcaaaact gctcgacttc ggcatggcgt tcaaggcggc tgacctcagc
     4681 gtcacccggc cgggaaatat cctggggtcc atacactaca tgtcgccgga acaggtgcgc
     4741 ggcgagcgcg tggacgcgcg ctccgacctc tactcgaccg gcgtaacgct ttacgaaatt
     4801 attaccggat accggccgtt cgatggtaag acggaatacg aggtcatgac cggacacctg
     4861 cgcgagcagg cgaagtggcc gggcggtatc gatcccgcga tcccctatgg actttcgacg
     4921 atcgtgatga aggcgctcgc caagaaccct gacgatcgct accagaacgc cgccgatttt
     4981 ctggtggcgc tgacccacat ttccctggac gacgcggcga ccttgcacac actcgccatc
     5041 cagccaagtg catcatcttc accttcagac ggtgtcaagc caccatccgg atctccggtc
     5101 agtaagccgg gatcagcgac gccgaagccg tcgaacaacg cgctcgatcc gtccatcctc
     5161 gactcggtca gccgcgagct tgctgcgttc atcggaccga tcgcgaaagt ggtggtgaag
     5221 cgtgctgctg gacggtgttc ctcggttgac gagttgtaca gcgccgtcgc cgcggaaatc
     5281 gactcggaca aagaccgctc ctctttcatg gccagcaaga agcggcgggt gtaaagcccg
     5341 agtcggggtt actgatctaa catcttcaat gctttcgcta gactgacgtg catacggttg
     5401 aacgacgcgg tcacgtcggc gatctcgtct ttgcctttca ccgtcaactc cggtaaatcc
     5461 gtatctccct tgctgatctg gttggcagcg ctggacacct tgtgcagcgg gcggatcacg
     5521 agcagataaa gcgcagcatc cagcgcgacg atggtgacca cgaaggtcgc gatcaggaag
     5581 aagagcagcc ggcgataggc atcatcggcg acgcccaggg gcacggacat gggcaccgaa
     5641 acaatctgcg ccgtgacgat ctggttaggc gtccagccaa acccattggc gctgccgtat
     5701 ttcctgatca tcgctgccgg cgcgacgtcg ggcgtgctgt ggcattccag gcaactcgca
     5761 gctaccgcga tgggtttggc caagtagagc tggcggccag ccggggtgtc gcgctcaccc
     5821 accagcttct tatcgccggg gtggtcggca aagtagcgga tgatgtcggt ttcccaatcg
     5881 tcggcgcggt ccaccaaatt ggtggggttc agcgtcgcct cgcggtacgc gtagttgggg
     5941 taggtcttgc ggatgctgtt gaagatggtc gtcgccgaaa acgcgggcac ggtctgcggc
     6001 aggaagttgg tttcgtgttc gggattcttc agcagcagcg gcttgatctg ctcggaggtg
     6061 tagtcgcggg tggcgcgggc gctggccatc atcaactcgg cctgctgcag cacctgttcg
     6121 cgggcattct gcgtgagaaa gctgtaggcc agatgcgcga tgatgaatcc tccggcgcca
     6181 aagaacacga tcaggacgag attgaacttc gtgagtagct tcatggattt gtagcagggt
     6241 acaccgtgcg caatctacct gcaaagggga cagtgagtcc cggaggaagc gtcctcgcgt
     6301 gatataaaat cttgcgcgat ttccaggttc gccaaccatc gttgtgacct ggttcgaccc
     6361 aatccgtcga taacgctatg aaatacctga tcacgcccgg tcgattcttt tttgcgattg
     6421 ccatcacggc gttcggcatc cagcacttca tttacgccat caccggtggt ggtctcggtc
     6481 ctccctggac gcccgccaac caccttctcg cgtacctcgt cggcgcagct ctcatcgctg
     6541 ccggcatcgg cctggcgaca ggcattcagg tgcgatgcgc gggaatcatg cttacgagtg
     6601 tggtcgtgat tcgagccttg ctttgctacg cgcccaagct ggccgccaat gtccgcgacc
     6661 ccggaccatg gacgagcgcg tttgaaatcc tcgcgatggg cggtgcgagc ctggtcttgg
     6721 ccgcgacctt cacaccccgg cgttcccgca ctgaccgaac cggcgctcgg cccggtgcat
     6781 tgttccaggc gggacggttt ctgttcgcca tttcactgat cgttttcggc atacagcact
     6841 tcttgtacgc caccttcatc gccacgctca tcacgccgtg gatccccggc cacctgttct
     6901 gggcgtattt cgttggcgtg gcttttattg cgtctgcgct cgcgatcgcg agcggcatcg
     6961 tcgcgccatt ggcggcaact ttgctgggca caatgttctt cctgtgggtg gttgtgcttc
     7021 acgcccccag ggtcgcggct gccgttcaga acgccaacga atggaccagc ctgctagtcg
     7081 cgcttgcgat gagtggcggc gcttttattg tcgctggagc ccttgcagac agggattaga
     7141 agtcggttct cctgcgggac gacccggtgt actttgctgc gtaccttatt cgcgcgatcg
     7201 aaatccgcgg ctgccaattg cgctttcctg cagcgagaaa aagccccgca tcgcaacggg
     7261 gctgtgttca gaaatcaggt agtttagttc cggcttctga acttcgtcag caaccgcaga
     7321 atctcgaagt acagccacac cagcgtgatc atcaacgcga acccgctgta ccactccata
     7381 tatttgggag cgttgaagcg cgcgccctgc tcgatcaggt caaagtcgag caccaggttc
     7441 atcgcggcca cgcctaccac caccaggctg aagatgatgc cgatgggccc tgagccattg
     7501 accgcattga agcggaagcc aaagaagccc aggacgaact ggataaggta gaacacggcg
     7561 ataccgccgg tcgctgcgac aattcccaat cgcaaattgt cggtcacctt gataaggccg
     7621 gagcgatacg ccagcaacat gacgatcagc gtgccaaagg tcagcgagac tgcctgaatg
     7681 gcgatgccgt ggtagcgaat ctcgaacatg gccgagacgc cgccgagcag cagtccttcc
     7741 agcaacgcgt agattggagc ggtgatcggc gaccactgct tcttgaagat ggtgaccatg
     7801 gcgacgatga atccgccgat accgccaatc gcgaccagag gggccatgcc cgccatcgct
     7861 tcttcaggtg aagtcgcggc aaagaaacgg ttccaggtga acgcagcgct ggccacgcat
     7921 agcagcacca ggatgcccgt cttgttcacg gcaccggaaa tcgtcatccc ttcgccgagg
     7981 gtgctttcca cggtacgaaa tgtgtcctgg cccagcgcgg gattcgatgt tctcatcaac
     8041 tgcggcattc caatttctcc ttgggtcgtc ttattggatg ttgcggatgg gttgttggat
     8101 gttcgtgctt attgtaaacg gtgctcaagg ccgccgttgg cgactaaaac agagtctcct
     8161 gccccgccgc gttcgtcttc ggacgcttga tcccgaaatg ctgatatgcc agatgcgtgg
     8221 caactcttcc gcgcggggtg cggtcgagaa agccaatctg gatgaggaat ggctcgtaga
     8281 tttcctcgat ggcgtcggct tcctcggcga gcgaagccgc cagcgtgttc acacccacgg
     8341 gaccgccctg gtatttctcg atgatggtca gcaggagctt gcgatcgatc tcatcgaaac
     8401 cgtagcgatc gacttcaagc atttcgagag cggactgcgc ggtgaccttg tcaatctttc
     8461 cgttaccgcg cacctgagcg tagtcgcgca cgcggcgcag taggcgattg gcgatgcgcg
     8521 gtgtaccgcg gcagcgggtc gcgatctcga tcgcggcggg ctcgtcgatt tcgacgccga
     8581 gaatttccgc cgagcgctcg aggatgatct tcaggtcggc gtgggtgtag aactccagcc
     8641 gcagcacgat gccgaagcgc gagcgcagcg gcgccgacag cagcccggcg cgcgtggtgg
     8701 ccccgataaa ggtaaacggc cttacctcga tggtatgggt gcgcgccgac ggaccctggc
     8761 caatgatgat gtcgagcttg taatcttcca gcgccgagta aagcaactct tccagcaccg
     8821 gctgaatgcg gtggacttcg tcgatgaaca acacctgctt gtcgcgcagg ttggtgagca
     8881 ctgccgtcag gtctcccttg atctgcaagg tcgggcccga ggtctgctga aacgccgcgc
     8941 ccagctcgtt ggcgatgatg ttggccagcg tcgtcttgcc cagtccggga ggcccataaa
     9001 gcagcacgtg atcaagcgct tcgccgcgtg acttggccgc ctggatggcg atctcgaggt
     9061 tgttctttac cttgtcctga ccgatgaact cgcgcagccg ctgtggacgc agctttaatt
     9121 cgaatgacgc gtcgtcatcg accggcgcgg ccgacacaat gcgctcgcga tccagactgt
     9181 ccttgggaaa aaccacgatg gggcgatggt aaagcgaaag gggaggcggg cgcaatgacc
     9241 gatcaacgtc ttcgacgtcc ttatctcgcg acggatcatg atccggtcga tcgcttacag
     9301 ttcagcaaca cttagtggtt ttcgattatt gtgccgcgcc gccctctccc atagcttcat
     9361 tccaatcgtt tatcggtggt tcaggaagcg gctgtcttgg gggagacggc cgcgaccctg
     9421 ttccgctgat ggggtccgta tttctttcta cgtggttttg caatttcatg atgacacgaa
     9481 ttctgtggcg ccagttcggg ctacgccaac gtactggtgc gcgacagtaa cacgccgggc
     9541 acggcaggat acctcacctc gtcgtcattc ggcgggccgg tcactacggc aggcggcgtg
     9601 ttcggtgcgg gaggaccacg agctttccaa cttgcgttac gatttagttc ctaaatggca
     9661 gagcaactca ttacgacgga cgcgcggccg gtccgcttgc gacgtgaact tggtctggga
     9721 tcggcagcgg cggcggtggc gggagaagcc atcgccgttg gcatctttct gacgcccgcg
     9781 ggcatggcca aacttctggg atcgccgttc tggctgctgt ttgtgtggct gctgatgggc
     9841 gccatcagca taagcggtgc gctgtgcttc ggcgaattgg cggcgcgtta tcccgaagcc
     9901 ggcggactct atgtgtacct gcgtgaggca ttcggcgaac gcgtcgcgtt tctctacgga
     9961 tggatgagca tgctggtcat ggaccccggc gtctccgccg cactcgccgt gggcatggcc
    10021 acctatgctt cttttatttt tggatggagt acgacagttg caaaactgtt tgccattgcg
    10081 tccatcgcag ccatttgcgg gctgaacatg atcaacaccc gcgcgagtgc tggatttctg
    10141 cgctacatca cttggctcaa gttcgccatc ctcgcgctgc tggtgatctg ggcgctggta
    10201 ttccggctgg gatcgtggtc acactttgtt ccgttcgtcg cccagcgcag cgggtcgact
    10261 cctctactgc cggcactggg aggcgcgctg gtagcggcgt tctactcctt cggcggatgg
    10321 tgggacgtga gcaaaatcgc gggcgaggtc aaagacccgg cgcgcactct gcctcgcgct
    10381 ctggtactgg gcgttgtcgg cgtcgtcgcc gcttacgtgc tggtgagcgg agtctttctt
    10441 tacctggtcc cgctgggaag cgtcaccaac gatcagacct tcgtagcgca ggcgggcgca
    10501 attctctttg gccgcacagg agccaatctg ctggcgggcg cggtgatcgt atgcgtactc
    10561 ggcagtctcg cggcactgtt catgctttgt cctcgcgtct attacgccat ggcgcgccat
    10621 ggagtattta ttcactcagt ggccgaactt catccggtgt ttgcaacccc tatgcgcgcg
    10681 atcgggatcc agggaatcat ggcttcgctg ctggtcgcct tgggcgcctt tgaccagatc
    10741 atcgcgtatt tcatcttcgt cgccgtgctg ttcatcggtc ttaccgtgag cacagttttc
    10801 gtatttcgcc ggcgcgaacg cggtgcagcg ccgacgatcc ggactccggg ctatcccttt
    10861 acgccgctgg cattcctggg catggtgctc ctgctgctga cgctcatgat gctgcgttcg
    10921 ccgcggcagg cgctgctggg gtgtgccgtg gtacttttgg gaatcccggt ctacgccttg
    10981 attcagcgca gacgcgaagc catgtcggca acgccagaag caattctcaa tcatgcactg
    11041 gatagacaat gagtgacgaa acgaaactcg ccgcacaaac caacgaacca gtgattcgcc
    11101 ctgcggtggc gggcgaggct ttgccgccaa cgaaaatacg catcacagaa gacgcggaag
    11161 ccataggtga cacgcttgcg gcttacgact actggcgcgc gggttccgga cgcacgcagg
    11221 ttccgggcat catcaaatgt ttcggctcgc gtcccgactt cctgcgccag gtggtggatt
    11281 tctccaacac cgttcatttc tctgaaggtc atctcgtgcg gcggcacaag gaaatgatcg
    11341 ccagctacgt ctcctacctg aaccgttgcc cgtattgact ggacagccac gcttacttcc
    11401 tgcgggttca gggagccaac gatgaaacgg ttgctgcaat tctggaaggc aaactcgacg
    11461 aggccagcct ggctccggcg gagcgcgcct taatggatta cgcgaagatc gtgaccgaag
    11521 cggcctaccg ctcgactccc caagatgtgc aaaagctacg cgaccacggc tggagcgaag
    11581 accaaatctc cgaagcggtc tacataatcg ctatgttcgc cttcttcaac cgcgtcgccg
    11641 atgccttcgg cgttccggca caacattacc tcgaactcgg aaaaatgacg ccgtaacggt
    11701 ctgggtttca gtgagtcccg gaatgtatga ttgcaccgtc catgaagaac ctcggcatac
    11761 tcctttccgg gcggggatcg aacttcgagg ccatcgctgg caacgtcgcc tcgggtaagc
    11821 tcgacgcgaa cctcgctgtc gtcatctcga acagggcgga tgcaacgggc atcgagtcgg
    11881 cgcggcggcg cgggctgaag gcgctggtca tcccctcgaa gggcaagccg cgcgaggagc
    11941 acgatcgcga gatggtcacc acgctgcgcg atcaccacgt cgatctggtc tgtctcgccg
    12001 gatacatgcg tctgctgtcg ccgtggtttg tgcagcagtt tcccaaccgc atcctgaaca
    12061 ttcacccgtc gctactgccg gcgtttcccg gactcgacgc ccagaagcag gccttcgatt
    12121 acggggtgaa ggtatcgggt tgcaccgtgc atttcgtgga cgccgacctc gaccatggcg
    12181 cgatcatcct gcagaaggtc gtacccgtgc tcgacactga cgacgatcac acgctggcgg
    12241 cccgcatcct ggagcaggaa cacatcgcct attccgaggc catccggatc gtgctgggag
    12301 gacagtacac catcaccgag cggcgtgtgg tcatgttaac atgaccatgg tcatggggag
    12361 ctggtcatga gcaagggtgt ggtagtcaaa aggacgaaaa agaaggtggc gaggcgcccg
    12421 cccggtcaga ggcagatggc agccggcgag ttcaaggcga agtgcctcgg ggtgctggac
    12481 gaggtgcagg ataccgggcg gcatattgtg attaccaagc gggggaaacc agttgccaaa
    12541 gtcgtgccga taaccgatgg aaaaaggaaa gacaacttcc tcggacggct ggaaggcatc
    12601 atgaagatca acggtgatcc tgacgacttg gtcaacccaa tattcccgct ggaagactgg
    12661 gacatgctga aatgattttg ttggataccc acgtcctggt ctgggcaaac ctggcgactc
    12721 ggaagttatc gcgaccggca gaatctgcca tccgacgagc gcgcggcagc gtggccattt
    12781 cagccatcag ccttctggag ttggcacggc tcatttccgc caatcggatt gaccggcgag
    12841 gaagtgtacg cgagacaatc gattcactca ctcagggaat cgagatcagg ccgatcaccg
    12901 cggaaatcgc tgccttgaca gcttatttgc cagccgactt tccatccgat ccctcagacc
    12961 gggcgattgc cgcaactgca caagcggaag gcattccgct ggtcaccgcc gacgagcgga
    13021 tacaagcttg ctcgctgatt agaatcatct ggtaattttc agtaagtcgc gctttacatc
    13081 tcgtagtgca gcagcgagaa cacgtcgtat tcctttaact tgtcgcggcc cttcaggccg
    13141 tcgagttcca ccacaaagct gagtccgagg atgtcgccac ccatggactt caccatttcg
    13201 gcggccgcgc gggctgtgcc gcccgtggcc agcagatcgt cgcagataag gacccgctgc
    13261 ccgggtgtga tggcgtcttt gtggatttcg agagtgtcgc tgccgtactc caggtcgtag
    13321 gtccacttca gcgtttccgc cggaagcttc ttgggcttcc gcaccggaac gaatcccgcg
    13381 ttgaggcgat aggccagcgc ggggccgaag ataaagccgc gggcctcgat gccgagcacc
    13441 aggtcgatgt ctttgtcgat gtagttctca gtaagcgagt cgatgaggcg ggcaaatccc
    13501 aacctgtcct taagcagggt ggtgatgtcg taaaacagaa tgccgggctt gggaaagtca
    13561 ggaacttcgc ggatgagcgt tttaagatcg tcgcagttct gcggcttggc ggacatgggc
    13621 ttaccacact ccttcgatgc caaacgattt cgcgtccaga aacggcgcag gcgacgccgc
    13681 gacatcgtcg acccgggcgg cgcgcggacc ctcccgcagc cggccatgca gggcgaccaa
    13741 ttgatcgcgg gtgcccgtgg ccatcacctc gacgctgccg ttttcgcgat tgcgtaccca
    13801 tcctgtgatg ttcaactggg ccgcttctcg ctccacaaac cagcggaagc cgacgccttg
    13861 tacgcgtccg gtaacaacaa agcggcgggt ttcgatgtca ggcatggaag gtgactggta
    13921 tctagcaaaa aaaagactta gcgatcgaca attcgcgtgg actccaattg taattgcgcg
    13981 aaagaagatt gtaatggaga gcgcgatccc acgggtgcga gtcgatccat tgcgcttcca
    14041 agcgcccaga caggcaccag gaatggtcgc agagcccatc cggaaaataa caagggcgcg
    14101 accgcagccg cgcccagttg aaccagttcc cgatcacgcc catcgcaacc actacatgtg
    14161 tttgggcgag atcttgtcga ggtacgagac caacggcttg cctgcggccg gcgttttcac
    14221 cgcgttctcc cgcacgatct gcgtagcgtt gagttccttg ccgtaaaggt tcttgttggc
    14281 gtcgtcgtcg gaacgcaggg tcgagcccgc cagggaaatt ccggcgaaca cgccgcgatt
    14341 ccgcgaccag gaaagaatct cagccttcat cacgatatcg gtctcagccg aggtggcacg
    14401 tccaaccgga ccagccgcga ctgacgcgtc tccgccgagc ttcactttgc tggtcaatac
    14461 cgattttgcg ccctgctcat tcatgatcag cagcacgtaa tcggtcgcct ggccgccggc
    14521 ctgcagaccg aagctcccgc cttcaatcgc atagaacgcc ggggctgtcc agagaccccg
    14581 gaactccggg ccggagcggc aggtcatcac gccgcggccg tagctggctc cgacaatgaa
    14641 cgcagccttc ttcaccgcgg gaaccacgat cacacattcc gccttgttca gcagatcgcg
    14701 gggaatgccc ttgtccggca taccggctac cgccttcagg acgtcatacg actctttcag
    14761 ccgtgaggtt tctttctctt gcgcgataag gggtaacgca agacaggtgc atagtacgac
    14821 agcaaaccac cgtttcatta tggttcctcc atgatgtccc ggcgatgcag tcgcacgggg
    14881 tacttaagat gggaaggccg cagtcagccg ttgcaggacc gctttggggc ggttggaacc
    14941 gcgtcccaag ccctgcgccc ggccagagaa aaacacagcg cggaaatccc ctgcagagta
    15001 tataggggcg cgacggaaag gcaaaaccaa aattctgccg taaaatttgg tccctgcgtc
    15061 gaacgcaggg accatggttc actcgcttag tctggagctc tcgcagcgac aatcgcgatt
    15121 acatgtgctt ttcggccgtc ttattcagca gatcgaccaa aggtttcccc ggagccggaa
    15181 cggcggcgtc gccccgtacg atctccatgg cgtcaatcga tttaccataa agtttttcat
    15241 tggcgtcatc gtcagctgcc atcgacgcgc tacccagagc cgcgcccgcg aacaagccct
    15301 tggcctccga atacgttagg acatcgacgt tcggatcgtt ccaaccgttg gcttgcgcgg
    15361 cgctggggcc tgctgcggct tgggcatctg cgcccagctt cagcttgccc gaaaggatct
    15421 tatcggcccc tctctgggtc atgaccagca ttacgtagtc ggtcgatgag gtgcctagct
    15481 gcactcccag gctacccgtg tccagggtgt acatgatggg agagccccag tggccattca
    15541 tcgctttgcc ggtgcggcag accaggacac ctcgcccgta agtcccaccg atgccgactg
    15601 ctacctttct cactgccgga taaaccagga tgcactccgc cttctcaaga agggttttgt
    15661 ttatcccgtc gggcttgctg agaagagtgt taagaaccgc ggttgactcg gatagacgct
    15721 tgtccacttt gctctgggca agggccggaa gcgtcacgat aaggcacagt acaacgagca
    15781 gtagcttctt catgtaggta acctttctta cacagtgcgt acacggacgt caatccggcg
    15841 ccgacgtacg caatagatgg gaacttggtt gtgttcggcc agtgaactca gcatctgaat
    15901 tgtagcgcga cggccccaaa acgagaaatc cggcaccctg cgccgcgcct ttgcaagcgc
    15961 cgtacagaac tgttaactca cgcgcgcgac aggtaggcgc cgtcagaggt gtccacacga
    16021 atcttttcgc cttcgttgat aaacggcgga acatatacga ccagtccggt ttcagtcttg
    16081 gccggcttgg tcacgctgct ggcggtggct gacttcaatc ccggctcggt ctcaacgatg
    16141 gtgagctcga ccgtctgcgg caactccact cccaccggct tgtcgtcgaa aaactcgacc
    16201 gagatctgca ggttagcggt caggtactcg accgcgtcgc ccagtacgtc cttaccgagg
    16261 tggagttgct cgtaattctc cgtgttcatg aacacgtgat tttcgccgtc ctggtagagg
    16321 tactccatct ggacctggtc aaccgtgatc ttgtcgatgg cgtcgccgga gcggaaacgg
    16381 tgctcgaaca tggctccggt gcgcagatta cgcaacttgg cctggatgaa agcgcgaaga
    16441 tttcccgggg tgcggtgctc aacgctgaac accgtgtgca ggtcattatt gtgcttgatg
    16501 accatgccgg gacgcatctg tgtggctgga atcgccattc gtaaaactcc tttatgactt
    16561 gggagagctc ctgcgcgaag cgcgggccca tcaggtaaac acttgatttt acatgggtgg
    16621 ggcgtaattt gtgaaatcgc agcaggctcg atgccacgcg cccgcgattc agggtaacat
    16681 ctttaccaga ggaattttgc atgagcgacg aaatcgaaga tgtagtgagc gagggcgaat
    16741 accgcccatc cggtagcaac acattggtca tcggcctggc tttgctagtg gtgggctttg
    16801 cggcgggagc gatcgctgcc gcgctgatga ccccgaaaac cggcaagcag atgcgccgcg
    16861 aactaaagcg caaagccgac ggcctgcgcg atatggtcga cgacttaagt gaccaggcca
    16921 gcgactgggt tgacaagggt tccgacctgg ccggcaaagc gtcggactgg gcggacgcgg
    16981 cgaaagaaaa agtcgcacct ctggccaaga agttccgcag ctagggtcgt ctcgccgcgc
    17041 attgctcatc actgcgcggc atcaatcaaa gcgcgagggt tctactcgcg ctctttcttt
    17101 accgcggcga tggctctgcg atcctgccaa ggcaggacac cgtgcggtca gccttttcgg
    17161 aaattgccct tggacctgcc tgtccccttc ccagactcta gacccagccg cgaggctgcg
    17221 tccgggcaaa gaatttccgc ctaaccacct aaagataagt gccattttgg cacttaagga
    17281 ctatatagtg cgcgctttag tatgcaatag aatgaagttc ccgaatacgc cactcgaggt
    17341 gccttgctgt tccgcgacta ttggccggta ggagcgattc aattcccgaa cgattcacac
    17401 gctaaagaat aatcagagga ttaaaacgat gcgaaagaaa gctccttttc tgccaggacg
    17461 ggctgccttc atctgcgcgg tcatgttctt gtcgttaacc atcgcgggcc aagcccagca
    17521 accgctccag gttttgcata accatgttcg ccccgccgtt tccagcggtc aagccgtacc
    17581 ggtgggtttg cttccgtctt cgcagcgcct gaatctcgcc atcatgctgc cggtccgcga
    17641 ccagggcgaa ctaacccgtt tcctcgatcg gctctacgat ccttccagcc cggattacca
    17701 ccagtttctt accgtggccc agttcaccga acaatttggc cccacccatc aggattacca
    17761 ggctgtgatc gctttcgcca aggccaatgg tcttacggtg accgatacac ctgccaatcg
    17821 tttgctcgtg gatgtcaatg gctcggttgc gcaaatcgaa aagaccttcc atgttgggat
    17881 gaaggtctat ctgcatccca cggaaaaccg cacattttat tcgccggacc gcgagccctc
    17941 cctcgatctc agcgtgccgg tgtcgcacat cgccggtctc aataacttct ccataccgcg
    18001 atccatgtta aagaaagggt cggccgcgca gcagaacctg accagcaacg taggttcagg
    18061 ccccggtggc gcgtacctgg gcagcgacat gcgcgcagcc tactacggcg gcaccgctct
    18121 caccggaacc ggtcaggcag tcgcgatggt tgagtttgac ggctacaaca tgagcgacgt
    18181 gaccgggacc tttgacggac acacctatac cgtacccatc aacaacgtgc tgattgacgg
    18241 cgccagcggc gggtcggacg gcgacgatgg cgaacaggtc ctggacatcg tgcaggcaat
    18301 ctcgatggcc ccaggcctaa gccagctccg cgtctatatt gcgcccatca ctacgtcgat
    18361 tggcgtgggc gatgtggaca tgttcaacaa gattgcgacg gagaatatcg ccaagtcgat
    18421 cagttgctca tggggctgga atccggccga cacggcggcc aacgatccca tcttccagga
    18481 gtacgcggcg cagggccaga atcttttcgt tgcctcggga gactccgggg cctacaccgg
    18541 ttcgaacgct accgattcca gcttcccggc ggaagacgtt tatgtcgtag ccgtgggcgc
    18601 aaccgatttg accaccaatg gagcgggcgg cccctggcag tcggaagtcg cctgggccga
    18661 cagcggcggc ggaccggctg acaatggctt cgccattccg agttggcaga taggggtagc
    18721 cgataccgcc aatggtggct ctaacaccat acgcaatgtg ccagacgtgg cagccgaagg
    18781 caatttcgac aactacctat gcgaccaggg ctcttgccag ggaggctggg gaggaaccag
    18841 cttcgctgcg ccgcgctggg caggattcct cgcgctggtc aatcagcaag ccgtgactaa
    18901 cactggctcg acgctcggct ttgtcaaccc ggcgatctac gcgatcggca agagtaccaa
    18961 ctacaacagc gacttccacg acatcaccag cggcaataac aacaacggca aaggcaaatc
    19021 gttcaatgcc gtggttggct acgacttggt gaccggttgg ggaagcccca acggacccag
    19081 cctgatcaac gccctggctg gagctactag tccaagcttt acgctctccg attcgcccag
    19141 cagtctgact atcacccaag gcgccgccgg gggcaccacc accgtcacgg tgaccgacct
    19201 caacggcttt aacggaagcg tgagtctttc agcttccggt ctgcccagcg gtgtaaccgc
    19261 ggcgttcaat ccgacgagca caaccacaac cagcgtactg accctgacgg ccagccgtac
    19321 tgccaacctc ggaaccgtca cggtgaccat caccgggacg tccggcagcc tggttgcctc
    19381 cacaactctt acgctcacgg tgcaggcaca ggcggtacca aacttcacca ttggcgcctc
    19441 gcccaattcc ttgaccgtag cacccggcgg caatggaacc agcacgatca ccatcaccag
    19501 tcagaacggt ttcagttctg cgaccaccct gacggctacc ggcctgccca gtggcgtgac
    19561 ggcggcgttc gcgccgaacc ccgtgactcc gccggcgaat ggccccataa cctccgtcct
    19621 caccttcacc gctacctcga cggcaactgt tggacccgcc acggtgacgg tgaccggaac
    19681 gtctggctca ctcgttcaca gcacgacgat tgccctaacg gtaggcacgt ctacagccca
    19741 aaccgcggtt tacaatgcca cgctcaaggc tccagggtgc gcaaccgtcg gtatttcgtg
    19801 tgactcgggt ccgtccctgc tgctgggacg cgatcatttg tccggcggcg cggaacccaa
    19861 tcaacccaac accattaaca attcctgcgc cgacggcacc accgggacgt tccactccga
    19921 tgagtccaac gatcgtatcc tggtggcttc caccagcggt agcgcgttga cccacggcaa
    19981 cacggcaacg gtcaccgcaa ccgtctgggc ttggagcggg ttctcctcgg attccgttga
    20041 cctttactat gcggccaacg ccaacagccc ctcatgggtc ctgatcaaga cgatcaaacc
    20101 caccaaggcg ggagcacaga gcgtgtcagc cacgtttacc ctctccaccg gcgcactgca
    20161 ggcggttcgc gcgcagattc gctaccaggg aaccgcgtcg gcctgcacgt tgggttccta
    20221 taacgatcac gatgacctga tcttcgcagt gcagtaatgc attgaatgga tgtaggtccc
    20281 gttgtctgac aaccattcga gccggctccc aaccagggcc ggctcgattt tttggacggc
    20341 gaacaatcgc ttgtggcggg caaaggaaaa gcccaggcca gcgccaactc aatccggcgc
    20401 gaacctgggc agcaattctc acatctggag cgagagttac ttagtcgacc cgctcagaca
    20461 cgtggacatg aaactcttgc ggtcatcgcc cttgaggttc ttcgcagtcg cttctttatt
    20521 gcaggacttc attttgtcct gctgagtctg cggcactcca gcggcactga ggcacgacga
    20581 catgaacgtc ttgcgttcgt cgcccgccag attcttggcc ttggcctgcg cgttgcagtc
    20641 cttcatcctc tgttgctgtg cagcctgcgc cgcgctcggc tgctttgtat cctgggccat
    20701 cacgggcaga gccaatagtc ccagagcaag taccaacgaa attttgcgtt gcagcatgac
    20761 acgtcccctt ttcacattgg attgaattgg gtcccgaggg ggagaccacc aagcgcgcag
    20821 cgattgtaca actccacgcg ccgggtttcg agtgagaaat cggataccgc gcgggagcgc
    20881 gatcagtaca gccggaagtt gacttccacg ttgatcatga ccgggacggc ctgtccatcc
    20941 ttgcgggcgg gctcgaactt ccacagcttg acggcctcga tggccttctc gtccagtccc
    21001 atgccaagcg cgcgctgaat gcgaatctgc tgcggccggc catccgcgct gaccaccagc
    21061 cagagcacca cggtcccctg gtatttggcc ttgcgggctt cttccgaata ctccggatcg
    21121 ggcgcataca gcgccttcgg ggcgctcact ccaccgccta cgcgataggc tccgccgcca
    21181 tagccgccac cgtgtcctgg accaacaccc ggtcccgttc ctgaaccaac accgccgccg
    21241 ctgccgctac caattccgcc gccatagcct gtgccgttcg agggcggtcc cagcactttg
    21301 gaaagcgggt cgcccagagc gcccactgtc ggaagcttga tttcaggcgg caccaccacg
    21361 cttggcgtca caaccagctt gggattctcg ttgcgcacca caatcgcggg cggcacgatc
    21421 tggtctttgg agagcttggg tagcgcgccc ttgggggcct gaagtttgtc gcgatcgcca
    21481 ccaccgccac caccacctgc gcgcgtcttt gataccggca acggcaggta cgggctgatg
    21541 tcggtcacca gtcccaccac ctgctgcttg atctcgtcct gatgggtcac aacccagtgg
    21601 cccgaccaga tcagcagtcc cgccgcgatc atgtgcagga taaacgaata gacgaatgtg
    21661 tccttccgtt gcggatagag accctctccc tcgccaaaaa gcgtgggcag gatatcgtgt
    21721 ttctgctcga cggtcggagg cttttcagtc tccgccttgg tcaaaaccgg tgtcgacatt
    21781 tctacctatt agtatagacc cagcctgaaa cccgaagatg cacaaaaagt agtacgtgca
    21841 ccgcggctct ccggattacg ttagacgttt tcgcgacgac aaatgtcaac gagttgtgaa
    21901 agcgagtgac gaatcgatct gagctgccgg gtgcgcctcg atattgaaaa gcgttttgat
    21961 gtttctgcat acgcagtgtg gcctcacctg ttagagctct gatggaacgt ggctgcccct
    22021 cgcctcctag gctgctcgcc agccaagatt gtcagaaaca attcgctctt gctcgtcatc
    22081 ctgtcgcagt tgccagattt gtttttttat gcctcgcttt gttttcttca gcttgggctg
    22141 aaattgttcg catctggtta attctattgg tggttatagc tatccatgtc tgccgtaagg
    22201 acttaggcga tgttttcggc atcctcggta gccggttgag cgtcagggct ggcggcgaca
    22261 ggtgcctttt ccggcgagtt tctgttcagc caatctctca gcagcgaggc gcatgttttt
    22321 ttccagggcg atggcggcgt agcgcttgcg gttggtgacg atgtggcggc gacgacggtt
    22381 gcgctgctgt tgcttgtggc ggaggcccat ggcttcgtcg ccttgggttc gctggatttc
    22441 gcggagctcg gcgagttccg ccgtctcggg gtagtcggcg cggatggtgt gctcggagca
    22501 gccagggccg tgcttgcagt aggcgacggt gggcatggga gagagggaag aagcaggtcc
    22561 ctcgactccg tccgccgaag cggcggcctg cgctcgggat gacgaatcag aaaaagcgat
    22621 tccgggcggc ggggggaagg cgagttcggg cgtgaggttg agatcaagat cgttgggcag
    22681 gccgaagtcg gcagtgagat cgacggcggg tgcgggctgg tcggagtggt aagggctggc
    22741 gggccacttg tcagacttga gcaggttgcg ggacgcgatg ttcagcacgc gaatgaggcg
    22801 gtcggcgcgc ttgagttcga tcatgtcggc ggcgagggcc tcggagagtt gggtgagcgc
    22861 cgattgcacg ccgaacgtgg attcgagcgg cgggagtatc aagtcgaagt gctcaccgcg
    22921 ggcgcggtac tgggccatgc gcatgagccg ggcacggtgc cggaggtggt agaggcaaag
    22981 gttacggtcg gtgacggcgg cagagttgca gatgccgcca tcgtcgtgga tgtaggtgca
    23041 agtacggtat tccatggcgc tccttaaaag cagtggtcag tggctagtgg ccagcagaga
    23101 cagtttcgag tttcaagttt cgagtttcca gtgaagccgc cgacaagccg gcaaccgtcc
    23161 gggcataggt ctccagtctt tagtacctat atcaacaggt ctttgccgaa tctggcaaaa
    23221 ctttcaggat gcagccgctg ccaatgcggg aatgatgtag aggtttttga gtttgagtcc
    23281 gagagcgat
//
LOCUS       DASRKK010000339         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_905800, whole genome shotgun sequence.
ACCESSION   DASRKK010000339 DASRKK010000000
VERSION     DASRKK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905800"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFC15_07440"
     CDS             <1..265
                     /locus_tag="VFC15_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10027.1"
                     /translation="FLVCQKEESVWRAFEAVYKQAESDRKFAKLIAGKARQVLAAKKK
                     SRALKSRVAAAPTQKTVDKLRRNIWEFSEEVRVHTMAAGEVAG"
     gene            513..1667
                     /locus_tag="VFC15_07445"
     CDS             513..1667
                     /locus_tag="VFC15_07445"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZM10028.1"
                     /translation="MIVAGVMSGTSADGINVALVRIQGRGFRSRFELLAHYEFPYPDN
                     VRRVILATMNAGSASVPDLSRLHFLLGELYADAVRAAQRRARLECELVGCHGQTLYHQ
                     GSPALFLGRRIACTWQAGEAAIIAAKIGVPVVSDFRPADMAAGGKGAPLVPFLDYALY
                     RHRRYGRIVQNLGGIANLTAIPPRALPQDVVAFDTGPGNMVIDAVTEHLFERPFDRNG
                     RIAARGDPIERVLQQLLRNPFFRQKPPKTAGREQFGREFVREFLRLCRRADNRDIVAT
                     ATALTAHSIGIAVRKFVLPLVEPPLRFRELVVSGGGTNNLTLMRMIREELAPLKMRVR
                     TSDDFGLPSEAKEAVAFAMLAYQTWRHLPSNIPSATGAERPAILGKVTYA"
     gene            1873..>2390
                     /locus_tag="VFC15_07450"
     CDS             1873..>2390
                     /locus_tag="VFC15_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM10029.1"
                     /translation="MLLNTGCSSKPDPNTLVMVIESSPTNLDPRVGVDAQSERIDELL
                     FDSLVRRDDHFNLKPWLADSWEMPNEQTYIFHLHHGVHFHNGQLLTARDVKWTFDSLL
                     SGKLRSAKTSTYAPVEDIDAPDDYTLVFHLKEPFAPMLWNLSDGAIGIVPYGSGDDFN
                     RKPIGSGPFRFVR"
BASE COUNT          489 a          724 c          687 g          490 t
ORIGIN      
        1 gttcctcgtc tgccagaagg aagaaagcgt gtggcgagcg ttcgaggcgg tttacaagca
       61 agccgagagt gacaggaaat ttgccaagct catcgccgga aaggcgcgac aggtattggc
      121 ggcgaagaag aagtctcgcg ccttgaagtc acgcgtggct gccgctccga cgcaaaaaac
      181 cgtggacaag ctccgccgca acatctggga attcagcgaa gaagtgcggg tgcataccat
      241 ggctgccggc gaggtagctg ggtgatcgtc gagccacgat ctccttgatc agacttgaaa
      301 gggcgcagct ttagctgcgc cgtattccac aaaaaggaaa gggcttcagc ccctgaggga
      361 cttcggttta tgaagtctgg ttgcacatcg acgacctcgt taacgtacat gaaaggcgaa
      421 tgccctcagc ggctgaagcc gattatcttg gcgaacgcct accgcacggc tgaagccgtg
      481 ccccttcaaa acggttcttc ggagcttgcg ccttgatcgt cgcgggagtg atgagcggca
      541 cctcggccga tggaatcaac gtcgcgctgg tgcgcattca ggggcgcggt ttccgctctc
      601 gcttcgagct gctggcgcat tacgagtttc cttatccgga caatgttcga cgagtgattc
      661 tcgcaaccat gaacgcgggc tccgccagcg tccccgatct ttcgcggctg cacttccttc
      721 ttggcgaact ctatgcggac gcggtccgcg ccgcgcagcg acgtgcacgt ctggagtgcg
      781 aactagtggg ctgccacggg cagacgctct atcaccaggg aagtccagcg ctcttcctgg
      841 gccgccgcat tgcttgtacc tggcaggccg gcgaagcggc cattatcgcg gcgaaaattg
      901 gggtgccggt ggtttccgat tttcgtcccg ccgacatggc tgccggcggc aagggcgcac
      961 cgttggtgcc ttttctcgat tacgcgctct accgccatcg ccgttacgga cgcatcgtgc
     1021 aaaacctcgg cggcatcgcc aacctcacgg cgattccgcc gcgcgcgctt ccccaggatg
     1081 tggttgcctt cgacaccggt cccggcaaca tggtgatcga cgctgtcacc gagcacttgt
     1141 tcgagcgccc cttcgatcgc aacggacgaa tcgccgcccg cggcgatccc atcgagcgcg
     1201 tgctgcaaca gttgctgcgg aatccattct tccggcagaa gccgccgaag accgccggcc
     1261 gcgagcaatt cggccgcgag ttcgtgcgcg agtttctgcg actctgccgc cgcgctgaca
     1321 accgcgatat agtcgccacg gcaacggcac ttaccgcaca ttccattggc atcgccgtgc
     1381 gcaagtttgt gctgccgctg gtagagcctc cgctgcgttt tcgcgaactc gtggtctccg
     1441 gtgggggaac gaacaatctg acactgatgc ggatgattcg cgaagaactg gcgcctctga
     1501 aaatgcgagt ccgcaccagc gacgactttg gacttccttc cgaagcaaag gaggccgttg
     1561 ccttcgcgat gctggcctac cagacttggc gtcatctgcc gtccaacatc ccatccgcca
     1621 ccggagccga gcgtcccgcg attctgggta aggtcaccta tgcataggtt ccttttcatc
     1681 acggagaaca cggagggcac ggagaaattc cagaagtcat ttaccacaga gacacagaga
     1741 cacagagaac gacaagaaga aaagatcaaa aagcccaacc aacttggacg acacgaccgc
     1801 agtgggtact gcccaagtcc cagatccaga ttttctctgt gcctctgtgt ctctgtggtt
     1861 ctactctgtc tcttgctctt gaacaccggc tgttccagca aacccgatcc caacacgctg
     1921 gtgatggtca ttgagagcag tccgacgaat cttgacccgc gggtcggcgt ggatgcgcag
     1981 tcggagcgaa tcgacgaatt gctcttcgat tcgctggtcc gacgggacga ccacttcaac
     2041 ctcaagccgt ggctggctga cagttgggaa atgcccaatg agcaaaccta tattttccat
     2101 ctgcatcatg gggtccactt ccacaacggg caactcctga ctgcgcgcga cgtcaagtgg
     2161 acgttcgatt cgctccttag cggtaaactg cgcagcgcta aaacaagtac ctacgcaccc
     2221 gttgaagaca ttgacgcgcc cgacgactac actctggttt ttcatttgaa ggaaccgttt
     2281 gcgccgatgc tgtggaatct gtccgacggc gcgatcggca tcgtgcctta cggcagtggc
     2341 gatgacttta atcgcaaacc catcggctcc gggccatttc gctttgtgcg
//
LOCUS       DASRKK010000340         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_612022, whole genome shotgun sequence.
ACCESSION   DASRKK010000340 DASRKK010000000
VERSION     DASRKK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612022"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFC15_07455"
     CDS             complement(<1..1241)
                     /locus_tag="VFC15_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10030.1"
                     /translation="MNARAAQRANNKQVTARRHSHAHPPSREELQRQVKQQQREIERL
                     RQQVVERDQRIADVQKQIADAEKQMLDAEKQIADLERQLATRKKNSTNSSKPPSSDGL
                     AGTQRRRCSPRKKSGRKPGGQPGHVGQERQRVEKPDRTEEVLPLQCKHCETALPQAPE
                     ERQTTGDVLCRQIVDLPEVILPVVTEFQYPKLVCPCCQKGTRAEVRSEHAHEIGERLT
                     AVVSYLISARKMTRRDVRATLQDLFGVEISVGSVQKAWEETADAVEAPYTELEEALAT
                     EPVLNSDETGSRTNGDKRWVWALCSSWFVFYHIACSRGVEVLVELLGEAFAGILCSDR
                     CPTYLSYHRGVAQFCWAHLQRTLKGIGEFASTADAVHFARDMLSAVKRLFGLWYQFCG
                     EAGIGKRLLTRTELIQQSIPI"
     gene            1852..>2104
                     /locus_tag="VFC15_07460"
     CDS             1852..>2104
                     /locus_tag="VFC15_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HZM10031.1"
                     /translation="MTRLRSLIGILGLSALAGPGLCRAADNNALKLWYRQPAGAWTQA
                     LPVGNGRLAAMVFGGAGKEHLQLNEDTVWSGEKRDRSNPE"
BASE COUNT          383 a          658 c          639 g          424 t
ORIGIN      
        1 tgaatcggga tcgattgttg aatcagttcg gtgcgcgtca acaaacgctt gccaatcccc
       61 gcctcgccgc aaaactggta ccacaaacca aacagccgtt tgacggcgga aagcatgtcg
      121 cgggcaaaat gcacggcgtc cgccgtggaa gcgaactccc cgattccctt gagggtgcgc
      181 tgcaagtgag cccagcagaa ctgggccacg ccacggtggt aactcaagta ggtcggacaa
      241 cggtccgagc agaggatgcc ggcgaatgcc tcccccagca actccaccag cacctccacg
      301 ccccgactgc aggcgatgtg gtagaacaca aaccacgagg agcacaacgc ccaaacccag
      361 cgtttgtcgc cgttggtacg gctgccggtt tcgtcgctgt ttaacaccgg ctcggtagcc
      421 agggcctcct ccaattccgt gtagggcgct tccaccgcat cggccgtctc ctcccacgcc
      481 ttctgcacgc tgcccacgct gatctccacg ccgaagaggt cctgcagggt ggcccgcacg
      541 tcgcgccgcg tcatcttccg cgccgagatc agatagctga ccaccgccgt caggcgctcc
      601 ccgatctcgt gggcgtgttc ggaacgcacc tcggcgcggg tccctttctg gcagcaggga
      661 cacaccagct tcggatactg gaattcggtg accacgggca ggatcacttc cggcaaatcc
      721 acaatctgac ggcagaggac atccccggtt gtctgccgct cctccggggc ctgtggcaac
      781 gcggtttcgc agtgcttgca ttgcaacggc aagacctctt cggttcgatc cggcttctcc
      841 acgcgttgcc gttcctgtcc cacgtgcccg ggttgcccgc ccggtttgcg cccactcttc
      901 ttcctcggcg agcaacggcg gcgttgtgtg ccggccaggc cgtcggagga aggaggcttg
      961 gaagaattgg tggaattctt cttgcgcgtg gccagttgcc gttccagatc ggcgatctgc
     1021 ttttcggcat cgagcatttg cttttccgca tcggcgattt gcttttggac gtcggcgatt
     1081 cgctgatccc gttcgaccac ctgctggcgg agtctctcaa tctcacgctg ctgctgcttg
     1141 acttgccgct gcagttcttc ccgactgggc ggatgcgcgt gcgaatgccg acgcgccgtt
     1201 acctgcttgt tgtttgcgcg ctgagcggct ctcgcattca tccacaatca agttaaacga
     1261 ttacgcgagg tttgtccaga gaaatcggaa gggacactgg gtgaactgcc gcaaaaccag
     1321 gggcgtgaac agttactcat gctgtctatc gcgctgcgtt ctctccagat tggaagcgtt
     1381 tacgccgatc gcctgtcgac ggttgcccga cccgcgcggg gcttttggca tgagagacgc
     1441 gggcattgtg ggggttaacg cgcggcaaca ttcccggtta accgttagct ggagcagccg
     1501 gcgggcgcca ccgttccata taagctacag attctgatga tattatgctc cggacacagt
     1561 accaaaccta gtctgcgggg gcactgcgcg ttacgattcc aatctaggct tatcagcgag
     1621 gctgggcgga ggaatgcgcc cctaatagtc gtgtaggggc ttgcaacgcc agcccccgaa
     1681 agtggaaatc ttgttccaac gtggcagcca atccaagttc tcccggaccg cgaggttcgg
     1741 agtccccgac tctcgctggt ccgttggatg ggagccgccc ggcctggagg gttcaagggg
     1801 gggaagtccg gtttgcctta acctttggaa cttgtactcg aaagccgaaa catgacgcga
     1861 ctccgatctc taatcggcat cttgggactt tcggcgctgg cgggcccggg cctgtgccgc
     1921 gctgcggaca acaacgccct gaagctctgg tacaggcagc cggccggcgc gtggacgcag
     1981 gctctgccgg taggcaatgg gcggctggcc gcgatggtct ttggcggcgc cggcaaggag
     2041 catctgcaac tcaacgagga caccgtctgg tcgggggaga aacgcgaccg gagcaacccc
     2101 gagg
//
LOCUS       DASRKK010000341         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_26336, whole genome shotgun sequence.
ACCESSION   DASRKK010000341 DASRKK010000000
VERSION     DASRKK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26336"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1284..1565
                     /locus_tag="VFC15_07465"
     CDS             1284..1565
                     /locus_tag="VFC15_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM10032.1"
                     /translation="MVPVVLTELLSDPELPADVSETLSELPLLEVEPGYWQRAGVLRA
                     KAIAKHRKARLGDALIAQSCIDRGAPLLTRDRDFRAFAEAADLDLVLKF"
     gene            complement(1628..2350)
                     /locus_tag="VFC15_07470"
     CDS             complement(1628..2350)
                     /locus_tag="VFC15_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10033.1"
                     /translation="MRIAGKFALSLSVALLCATPGLWSEDIGVSGRVVAEGGGNLSPA
                     SSTVVWLAPIAAAPGDSAVVPPMHAVLQQKNKAFEPHLIVVTKGSTVDFPNRDPWFHN
                     VFSLFNGKRFDLGLYEAGTSRTVHFDREGVSYIFCNIHPEMSAVVVVLSSQYFAVPNK
                     QGEFSIASVPPGRYMLHVWNENALPATLQALSREVMVSETSRSLGLIHVRVTLATSAP
                     HKNKYGQDYQPPSPNNPIYVQP"
BASE COUNT          526 a          659 c          709 g          536 t
ORIGIN      
        1 cacgggtact cttcaggcaa taacttccag tctggtatga acggataggg aaaggtcaaa
       61 tgcttgaagg gcagagttaa cggcacgaac ccaagaggcg gagatgtaga atgccccggc
      121 tgttcgtaga acttgcctaa aatgaagtcg aggtgacggg ccggattgtc ggtcacttcg
      181 catttgaaca gggccgaaaa cttgcgtaaa gcgtggcatc tgtggcggcc tgtcaagcaa
      241 cgtagctgtg ttggaagagt ttttgcaatt gattgcagag acttcgacgg ctgtctgctc
      301 cagttcagaa acaccgggta agaatgtcgg cggctgcggc tgcgggtctg tcagtgacct
      361 gtgccgcaac gggaagtgat tgattatttt cggcaagatt ctgacagcgg catcaagatt
      421 tgtggaatac cccacgctcc tcaacgcact agccttaggg tggtggattc catgcatgga
      481 cgactcccgc tgattctgct gttgtgcctg ttgttcgccc caacttcctc actgctccaa
      541 actcacgctc cccctcctag ctgcgagtct gcctcggcat ttgtcagcgc ggcccggtca
      601 caggtgcgtt tggtctatag caaatctggg gcggagtcct tagctgctct gttggcacac
      661 cgcagtgggc tttgatcgcg ggaacatgtg agagagttta tgaaatctat attcgcattt
      721 gccatagtag caatgggtgc acgaacgacc ttcttgccct tcttgccgcg agaggcaaaa
      781 tccaaaacct ccgccccgag agcacgcaga cggttgtaaa cggtcccccg tgagcaacgc
      841 cagcgttgcg caagttctgc gatcgagaag taatcctgct gtgcctctcc aggatgattc
      901 atttgcactt gccctagtag ttgaggcaag ccgataatga agtctgtgga ctgacaatac
      961 cggcttgccg ggttgttggt ttggatcctc tgctagttga cgctagtcgc gggtctattc
     1021 acgtaagtgc atcacgaatc ctgtcgccgt ttaattccgt tatctgaggg tattctccag
     1081 ccccgaacga cgaacgacgc gacaaattta gaggagatcg actgccgacg tggaacgtct
     1141 atgcggcggt cttgacgttg tttcgggtgt gacgcacctg tgacgcaaat cctgccaaca
     1201 gtgacattgc tgacatgttt gacaactcaa cagccctata aaaccgtaag ctgttgaatg
     1261 tgcgtggtgc gcggatctta ctcatggtgc ccgtcgtgct tacggaactc ctaagcgacc
     1321 ctgaacttcc ggcagacgtc tccgaaacgc tctctgaact gccgctgctg gaggtagaac
     1381 cagggtattg gcaacgggcg ggagtactgc gcgcaaaagc aattgccaag caccgaaaag
     1441 cgcgcctcgg agacgccttg attgcccaaa gctgcattga tcggggtgct ccccttctca
     1501 cgcgtgaccg ggacttccgc gccttcgcag aggctgcgga tttggatctg gtgctgaagt
     1561 tttgagcgac tatcagccac cgccgtaccg cgaatctggg gcacaagcgt acattcaaac
     1621 cctcaaatca cggctggaca tagatcggat tgttgggaga gggcggctgg taatcctgcc
     1681 catatttgtt cttgtgcggc gcagacgtgg ccagcgtcac ccgcacgtgg atcaaaccca
     1741 gcgagcgaga agtctcgctt accatcacct cgcgcgagag cgcctgcagc gtggcgggca
     1801 gcgcattctc attccacacg tgcagcatgt aacggccggg cggtacggag gcaatcgaga
     1861 actcgccttg tttgttgggg accgcaaagt attggctgga caacacgacc accaccgcac
     1921 tcatctcggg atgaatgttg cagaagatgt acgacactcc ttcgcggtcg aagtgaacgg
     1981 tgcggctggt tccagcctca tagagtccga gatcgaaacg cttaccgtta aacaaggaaa
     2041 acacgttgtg aaaccaggga tcgcggttcg ggaaatctac ggttgagccc ttggtgacga
     2101 cgatcaggtg cggctcgaat gctttgttct tctgttgcag cacggcatgc atcggaggaa
     2161 cgacggcgga gtcgcccggc gcggcagcaa tcggggctag ccagacgacg gtggagctgg
     2221 caggggacag attgccgccg ccttcggcca ccacgcggcc ggatacgccg atgtcttccg
     2281 accacaagcc tggcgtcgcg cacagtaacg cgacacttaa cgacagcgcg aattttcccg
     2341 caatacgcat aggtccgtcg gtgccggttc caatgttggt gtgcccgggt agagccggcc
     2401 ttcaggccgg cgtcgaggag cactcacttt
//
LOCUS       DASRKK010000342         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_515268, whole genome shotgun sequence.
ACCESSION   DASRKK010000342 DASRKK010000000
VERSION     DASRKK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515268"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFC15_07475"
     CDS             complement(<1..748)
                     /locus_tag="VFC15_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZM10034.1"
                     /translation="MHRVYLDNNATTPLLPEVFEAMRPYFFEKFGNASSIHQQGQQAR
                     SAVEHAREQVAELLNCRPAELVFTSGGTEGDNLALFGLMKPGDHIITSAVEHHAVLNA
                     CKRLEEMGCAVTYLPVDGRALVDPDDVRRALRPQTKLISIILANNETGVLQPISEIGK
                     IATEADVYFHTDAVQAAGKVPLDVKEIGCDLLTITGHKMHGPQGAGALFVRRGTLLQP
                     MMYGGRHERSRRAGTENLPGIVGLGKAAEIA"
     gene            868..1464
                     /locus_tag="VFC15_07480"
     CDS             868..1464
                     /locus_tag="VFC15_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZM10035.1"
                     /translation="MKITFFRSASVFRDWLASNHHKVQELCVGFYKKKSGKDGISYPE
                     AVDEALCFGWIDGIKKAVDDVSYTHRFTPRKPKSIWSAVNIERVGELERLGRMMPPGL
                     KAFAGRDEQKAMQYSYERSTCKLEGAYEKQFRGNAKAGEFFQAQPPGYRRTATWWVIS
                     AKREETRGKRLATLMETSERGHRLDMLGPQTKKTSRPK"
     gene            complement(1578..>2373)
                     /locus_tag="VFC15_07485"
     CDS             complement(1578..>2373)
                     /locus_tag="VFC15_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZM10036.1"
                     /translation="VNDHEANMRKAVRLTAQIAYVVAAYDRIRKGQPLIEPDRSLSHA
                     GNFLYMLNGEIPSPTAERAFDIALILHADHELNASTFAARVVAATLSDMHSAVTAAIG
                     ALKGPLHGGANEAVFRILEAVDASGADPVDYVKGMLAQKKKVPGFGHRVYHTEDPRAT
                     HLRTMSRDLGQSSGNTKWFEISRKIEEFIVADRKLNANVDFYSASTYHTLGIDIDLFT
                     PVFAVSRVAGWTAHVIEQLNDNRLIRPRADYQGPAYPNKYVPMEKR"
BASE COUNT          489 a          686 c          714 g          484 t
ORIGIN      
        1 gagcaatctc cgccgccttg ccaagtccaa caatgccggg caggttctcc gttccggccc
       61 gccgagaacg ctcgtgacgc cccccataca tcatcggctg cagcaaggtc cccctgcgta
      121 cgaacagtgc cccagcgcct tgcgggccgt gcatcttatg gcccgtgatg gtcagcagat
      181 cgcagccaat ctccttgacg tcgagcggca ccttgccggc ggcctgcact gcgtcggtat
      241 ggaagtagac gtcagcttcc gtcgcgatct tgccaatctc gctaatcggc tgcagcacgc
      301 cagtttcgtt gttcgccagg atgatgctga tgagcttggt ctggggacgc agcgctcgcc
      361 gcacatcgtc gggatcgacc agcgcccgcc catccacagg gaggtaggtc acagcgcaac
      421 ccatctcctc aagccgtttg catgcgttta gcaccgcgtg atgctcgacg gccgaggtga
      481 tgatgtgatc gcccggcttc atcagcccaa acagcgccag gttgtcgccc tcggtgccac
      541 cgctggtgaa gacgagctcc gccggacggc agttcagcaa ctccgcaacc tgctcgcgcg
      601 cgtgttccac cgcggagcgt gcttgctgtc cctgctggtg aatggacgac gcgttgccga
      661 acttctcgaa gaagtaggga cgcatggcct caaacacttc gggaagaagc ggagtggtgg
      721 cgttgttgtc gaggtagacc cggtgcattt ctggctctat tctggtttct attctaacgt
      781 cttagttaga ttcggggcgg ggaagcgggg attcggagaa gagcctgtcg ctcctcggtt
      841 gtactgctcg gccaaatgga gcttgccatg aagattacgt tcttccgatc agcgtctgtt
      901 tttcgagatt ggttggcgtc gaatcaccac aaagttcaag aactttgcgt tgggttctac
      961 aagaaaaagt cgggcaaaga cggtatcagc tatccagaag ccgtggatga agcactctgt
     1021 ttcggctgga tcgacggaat aaaaaaggcc gtggatgatg tcagctacac ccaccgcttc
     1081 accccacgaa aaccgaagag tatttggagc gcggtgaata tcgaacgcgt cggcgagttg
     1141 gagcggcttg gacgcatgat gcccccggga ctcaaggcat tcgcaggacg tgacgagcag
     1201 aaggcaatgc agtattcgta cgagagaagc acgtgcaaac tggagggcgc gtatgagaaa
     1261 cagtttcgag ggaacgcgaa ggcgggggag ttcttccagg cccaacctcc agggtatcgg
     1321 cgaacggcaa cttggtgggt gattagcgcc aagagggaag aaacgcgagg caaacggctc
     1381 gctacactga tggaaacctc agagcggggg cacaggctgg acatgctggg gccccaaacc
     1441 aagaaaacgt cccgcccgaa gtagttgatg aagggtgatg gcaactccat caaatcatct
     1501 ttgtccgcca ttccctcttc gcaaaacctg gcttggcaaa tggcgaaaag cgaaaagcga
     1561 atagcgaaca gcgactccta ccgcttttcc atgggtacgt atttattcgg atatgccggc
     1621 ccttggtaat ccgcgcgtgg ccgaatcagt cggttgtcgt tgagctgctc gatcacgtgc
     1681 gccgtccacc ctgccacacg cgacacggca aacaccgggg tgaaaaggtc aatgtcgatg
     1741 cccagcgtgt gataggtgga cgcggaatag aagtccacgt tcgcgttcag cttgcgatcg
     1801 gcgacgatga actcctcgat cttgcgcgag atttcgaacc acttggtgtt gccgctggac
     1861 tgtccgagat cgcgcgacat cgtccgcaga tgcgtggctc gcggatcttc cgtgtgatac
     1921 acgcgatgcc cgaatccggg caccttcttc ttctgcgcca gcatcccttt cacgtaatcg
     1981 accggatctg ccccagatgc gtccacggcc tcgaggatgc ggaacaccgc ttcgttagcg
     2041 ccgccgtgta gcggcccttt cagcgctcca atggccgcgg tgacggctga gtgcatgtcg
     2101 ctgagagtcg ccgccactac ccgcgccgcg aaggtcgagg cattcagctc atgatcggcg
     2161 tgcaggataa gcgcgatgtc gaaagcacgt tctgccgtcg ggctcgggat ctcgccgttc
     2221 agcatgtaca ggaaattgcc tgcatgcgac agcgagcggt cgggctcgat cagcggctgg
     2281 cctttgcgga tacgatcata ggcggcgacc acgtatgcga tttgcgccgt cagtcgaaca
     2341 gccttccgca tgttcgcttc gtggtcgttc acc
//
LOCUS       DASRKK010000343         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_808112, whole genome shotgun sequence.
ACCESSION   DASRKK010000343 DASRKK010000000
VERSION     DASRKK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808112"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFC15_07490"
     CDS             <1..359
                     /locus_tag="VFC15_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10037.1"
                     /translation="LSSRHLLFLRLSTSRYSGTNNVFFDPASPITTYAESNNGSEDVK
                     TESLAASWTSAWTHRLSTNLRAQFSRDVQQSIANSDDPKIKIYGLVDGMGRSNILPRD
                     TREHKLLTPSATTPGE"
     gene            356..2047
                     /locus_tag="VFC15_07495"
     CDS             356..2047
                     /locus_tag="VFC15_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM10038.1"
                     /translation="MNWKFGGDFLQAWIYNYDPAMFGGEYYFDNVKVNPWFFTPQKNG
                     EPLTPLSAHAHGVPRYYMQDFGNSVSHPNSRSYSAFVQDSIRITRSFTVNAGVRYDLQ
                     TFEVPGLESNPLYAPAGKVPTDTNNFSPRVGFTYALHERHPIVLRGGFGRFYSQVPNI
                     YASQVENDNGLTRSQLFLDIMNPANAAIFPKYPNTLVNCPAGATICTPPASVASQLTS
                     QVSAFSPNFQTPYTEQASLTLEYGLGANLTASASYLYVHGEHLIRSLDVNLPKPKITE
                     YPVYNDQSVFTGDYYSVASFATWQTTRSVDCPYPPWINDVHRPDPRLGTINSFESAAS
                     SVYNGLTLLVKGKIGKQVFVHVGYTFAKAVDDGTDALVVDRPGNVQNAYATQLERGLS
                     VTDQRNRFIASSVYEPQSFHYEQPVLNALFNNWKVSTVFTVGSGRPINATMAGDANRD
                     DNTYNDRLPGVVRNAYIGPGYFTTDVRISKGFQLSERVRLRLLAESFNVTNRVNQRVD
                     ISDDGFLNSAGQFIAYSTSIGKTSYPGEFVKNSKFLIPTNSYAPRQVQFSLKASF"
     gene            2166..2390
                     /gene="rpsU"
                     /locus_tag="VFC15_07500"
     CDS             2166..2390
                     /gene="rpsU"
                     /locus_tag="VFC15_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZM10039.1"
                     /translation="MDEFGGVFPLAEVRIQEGESLENALRRFKRKVQQEDIIKEVKRH
                     SFYLKPGEKKRVKEALARKRSRKKARKEQD"
     gene            2645..2950
                     /locus_tag="VFC15_07505"
     CDS             2645..2950
                     /locus_tag="VFC15_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HZM10040.1"
                     /translation="MEFQDKVLKCIDCGSEFVFTAGEQLFFHDKQFKNEPKRCKPCKA
                     KRAANVGPTSAGNYSKVETRTNCSGCGKETTVPFKPTQGRPVFCRECFQQKRTAATA"
     gene            3062..>4003
                     /locus_tag="VFC15_07510"
     CDS             3062..>4003
                     /locus_tag="VFC15_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM10041.1"
                     /translation="MAQSGARSIGLRFSHPYNDEVGTYFVENFGCRATQADGAAIERQ
                     LLDRGLARAVSTSEAEVVVLNTCTVTASADQDVRSTIRRIHRENPATRILVTGCYAQR
                     APEEISALPGVSLVVGNSHKHEVADYALPLTTAAGFVPVTQIGPARDATVIIGDIFAH
                     TELLAAPVFDGEDSAEKTRPNLKVQDGCNNRCSFCIIPFVRGRSRSLQLEHVIRETNA
                     LVTRGYREIVLSGINLGRWGRELTPRMNFTGMLRAILENTAIEKLRISSVEPMDWSNE
                     LIELVASSPRIAKHAHVPLQSGSDKILRRMHRKYRPWH"
BASE COUNT          857 a         1195 c         1112 g          839 t
ORIGIN      
        1 atctttcatc gcggcacttg ctgttcctgc ggctcagcac gtcgcgttac tcgggaacca
       61 acaatgtgtt ctttgatccc gccagcccca tcacgaccta cgccgagagc aataacggta
      121 gcgaggacgt gaagaccgaa agcctggcgg catcatggac cagcgcgtgg acccatcggc
      181 tgtccactaa cctgcgggcg caattctcgc gcgacgtgca gcaatccatc gccaactcag
      241 acgaccccaa gatcaagatt tacggcctgg tcgatggcat gggccgctcc aacatcctgc
      301 ctcgcgacac ccgcgagcac aaactgctga caccgtcagc tacaacaccg ggcgaatgaa
      361 ctggaagttt ggcggcgatt tcctgcaggc ctggatctac aactacgatc ccgcgatgtt
      421 tggcggcgaa tattactttg acaacgttaa ggtcaacccg tggttcttca cgccgcagaa
      481 gaatggtgaa ccgctgacgc cgctgagcgc ccacgctcac ggcgtgccgc gctactatat
      541 gcaggacttc gggaactcgg tttcgcatcc caactcgcgg tcctattcgg ccttcgtgca
      601 ggatagcatc cgcataaccc gcagcttcac cgtgaatgcg ggtgtgcgct atgacctgca
      661 aacctttgag gtccccggcc tcgaaagtaa tccgctttat gcgccggcgg gaaaggtgcc
      721 taccgatacc aacaattttt ctccgcgagt gggattcaca tatgccctcc acgagcgcca
      781 tcccatcgtt ttgcgcggcg gctttggacg cttctatagc caggttccga atatctatgc
      841 gtctcaggtt gagaatgaca acggattgac gcggagccaa ttgtttctcg acatcatgaa
      901 cccggcgaac gcggccatct ttcccaagta tcccaatacg ctggtgaatt gccccgcagg
      961 cgcgacaatc tgcacgcctc cggcgtcggt cgccagccaa ctgacctcgc aggtgtccgc
     1021 cttctcgccc aacttccaga ctccctacac cgaacaggcg agcctgacgc tggagtacgg
     1081 cctcggggcc aatttgactg cctccgcgag ttatctctat gtccacggcg aacatctcat
     1141 ccgttcgctc gatgtgaatc tacccaagcc aaagattacg gagtatccgg tgtacaacga
     1201 ccagtcggtg ttcaccggcg actattattc cgtcgcgtcg ttcgccacgt ggcagaccac
     1261 gcggtcggta gactgcccgt atcctccgtg gattaacgac gtgcatcgtc ccgatccgcg
     1321 actgggcacc atcaactctt tcgagagcgc tgccagcagc gtatacaacg gacttacgtt
     1381 gttggtgaaa ggaaagatcg ggaagcaggt attcgttcac gtgggttaca ccttcgccaa
     1441 ggctgtcgac gacggcacgg acgcgctggt ggttgaccgt cccggcaatg ttcagaacgc
     1501 ctatgctacg caactcgagc gcggactcag tgtcaccgat cagcgcaatc gcttcatcgc
     1561 gtcatcggtc tatgagccgc agtcctttca ctacgagcag cctgtactaa atgcgctgtt
     1621 caataattgg aaggtctcga ctgtgtttac cgtcggctcg ggacggccca tcaatgcgac
     1681 catggcgggg gacgccaatc gggatgacaa cacgtacaac gatcgcctgc ccggagtagt
     1741 gcgaaacgcc tacattggtc ccggatattt caccaccgat gtccgcatca gcaagggttt
     1801 ccagttaagc gaacgggtgc gtctgaggct gctggcggaa tcgttcaatg tcaccaatcg
     1861 ggtgaaccag cgcgtggata tctccgacga cggattcttg aattccgcag gtcaattcat
     1921 cgcctattcc acgagtatcg gaaagacgtc gtatccgggt gaattcgtga agaattccaa
     1981 gtttctgatt cccacgaatt cgtatgcgcc aaggcaggtg cagttttcgt taaaggcaag
     2041 cttttgagcc tcttgggacg tcaacagaca cctttgtgca aacccggtac cgcttaaatg
     2101 gggcatcgca gcgggactct taacttgact tccccggtga acattgggat aactgatatg
     2161 tccgtttgga cgagtttggg ggtgttttcc cgttggccga agttcgcatt caagagggtg
     2221 aatctttaga gaacgccctg cgccgcttca agcgcaaagt gcagcaagag gacattatta
     2281 aggaagtgaa gcgtcactcc ttttacctca agccgggcga gaagaagcga gtgaaggaag
     2341 cgttggcgcg caagcgcagc cgcaagaagg ctcgcaaaga gcaggactaa ttcagccgca
     2401 gggtcgctgt cacaaacgcg gccctgcgct tttacttttg agccgttgtt gggaaccttt
     2461 atatctggaa ttaccgcagt tttaactgca gtgaccatgc cgtccgcatg gtgggccgaa
     2521 cccaggggta gtagctgagc gatgggcttg cgatgccgtc ttggaccctc agtccggtcc
     2581 atgcgggcac gtgcaatcga tcccctcgat cgctcggatt atggactgaa gggagcaact
     2641 ctccatggaa ttccaggaca aggttctaaa gtgtatcgat tgtggcagcg agttcgtgtt
     2701 cactgctggg gaacagcttt tcttccacga taaacaattc aagaacgagc ccaagcggtg
     2761 caagccctgc aaggcgaaac gggcggcaaa cgtgggcccg acttcggccg gcaactactc
     2821 caaggtcgag actcgcacca attgttcagg atgcggcaag gaaacgaccg tccccttcaa
     2881 gccaacacaa ggcaggccgg tgttttgccg cgagtgcttc cagcaaaagc ggactgcggc
     2941 cacggcctga aagccggctg caacatcttc tgcatatctc acgactctcg cgagacgtag
     3001 gcgacagttc acagcctcgc tcgtttacct cgactgcccc gcgagtcccc agctatgagt
     3061 cttggcgcag agcggtgcgc ggtccatcgg actgcggttc agccacccgt ataatgacga
     3121 ggtggggacg tacttcgtag agaacttcgg atgccgcgcg acccaggcgg atggcgcggc
     3181 cattgagcgc caattgctcg atcgcggact ggcgcgggcg gtctcgacga gcgaggccga
     3241 agtcgtcgtc ctgaatactt gtaccgttac agcttcggcc gaccaggacg tgcgctctac
     3301 catccggcgg attcatcgcg agaatcccgc cacgcgaatt ctggtcacgg gttgttatgc
     3361 ccagcgcgct cccgaagaaa tttctgcctt gccgggtgtg agcctggttg tgggaaattc
     3421 gcacaagcac gaagtcgccg actacgcgct cccactgaca accgccgccg gattcgtacc
     3481 agttacgcag atcggcccgg cccgggacgc gaccgttatt atcggcgaca tcttcgcgca
     3541 caccgagttg ctcgccgcgc ccgtcttcga cggcgaagac tccgccgaaa agacgcggcc
     3601 caacctcaag gtgcaggacg gctgcaataa ccgctgttcg ttttgcatca tcccattcgt
     3661 tcgtggccgc agccgatcgc tgcagcttga gcacgtaatt cgcgagacca acgcgctggt
     3721 tacgcgtggc taccgcgaaa tcgttctttc cggtatcaat ctcggccgct ggggacgcga
     3781 actcacaccg cggatgaatt tcactggcat gttgcgcgct atccttgaga ataccgccat
     3841 cgaaaagctg cggattagtt cggtcgagcc catggattgg agcaacgagc tgattgagtt
     3901 ggttgcctcg tcgccgcgca ttgccaagca cgctcatgtc ccgctacagt cggggagcga
     3961 caagattctc cgccgcatgc accgcaagta tcgcccctgg cac
//
LOCUS       DASRKK010000344         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_319463, whole genome shotgun sequence.
ACCESSION   DASRKK010000344 DASRKK010000000
VERSION     DASRKK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319463"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFC15_07515"
     CDS             complement(<1..640)
                     /locus_tag="VFC15_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10042.1"
                     /translation="MDTQNDTTPFTAQTTNSSGQIIPGVPVTISVGTSLTFEVVSPGL
                     ATTLVYDQSGNVLDFVDNATEAVSGSVTLPDFAQMALFSPDASKVYAPVRNIPIAGLR
                     PGAVEVIDVASFAITANLSVPSVRYIALSPSGQYLLAFADDSDSVFLINTTASSVSAV
                     EIPGFARPVNAFFSADSNTAYVISCGVECGSTSGASVAQFDIPSQTIKATLPV"
     gene            953..2806
                     /locus_tag="VFC15_07520"
     CDS             953..2806
                     /locus_tag="VFC15_07520"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HZM10043.1"
                     /translation="MTADLISRLQKGAVLCDGAMGTLLYSKGIFINRCYDELNVSQPD
                     LIRGIHQEYLQAGADIIETNTFGANSFRLSRHGCVDKLADVNRQGARLAREAAKSFDA
                     LVAGAVGPLGVRIEPLGKVSLDEAREAFHEQIAALNEEGVDLLILETFGYLEELHQAI
                     LAAREVNPKLPIVAQVTIDEDGNCLDGSAPEHYGPQIEAWGADVVGCNCSVGPVAMLD
                     AIERVRAVTTAPLSAQPNAGMPRSVEGRNIYLCSPEYMAEFARRFVDVGVRLIGGCCG
                     TTPDHIHVMKAFLRAVSGGTLARKPAALAKAQPVEVPPLRERSQLGRKIADGEFVTMV
                     EIVPPRGTDGSREIEGARFLKSVGVDGVNIPDSPRASARMSNQALCLLVQQQVGIEPI
                     LHYTCRDRNVLGIQSDLLGASAVGIRNLICITGDPPKMGDYPDATAVFDVDAIGLVNI
                     VHNLNRGLDIGGNPIGGSTKFVIAVGANPGSPNIDEEIRRFQFKVEAGAELAVTQPVF
                     DIRLLEEFLRKIEHCRIPVVAGIWPLTSTRNAEFMKNELRVSIPDEIFTRMAKATTVD
                     AARAEGVAIARDMLAAVRHMVQGAQIAAPFGRYSCAVDVLEALGTGKRTAV"
     gene            complement(2910..3521)
                     /locus_tag="VFC15_07525"
     CDS             complement(2910..3521)
                     /locus_tag="VFC15_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10044.1"
                     /translation="MIPEAPVHIAEIDFPQLYAEVFGSLDSSTKFYETIRSLPDSKKD
                     AKIVFHQAGRMVWLADKIDEVARGRPAFQILFYLIAAELVAKIAAGYEGEGQSRSHVQ
                     KFFTEICSSESKQRLSRAFRHAGGVHLSLTSAVDLLYDVRCDVVHRGLYYTFSLQRSG
                     SSMPMLSNVHEYSPIAEISLLELRQIVLEGAALAAKRIAEIGE"
     gene            3707..3946
                     /gene="xseB"
                     /locus_tag="VFC15_07530"
     CDS             3707..3946
                     /gene="xseB"
                     /locus_tag="VFC15_07530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZM10045.1"
                     /translation="MPKFEESLQRLEKIVTELEKGDVSLDHALELFDEGMKLSSSCRK
                     ELEEAEGKVEILLKRNGKLHPEAFEPASQPGDTAK"
     gene            4028..>4354
                     /locus_tag="VFC15_07535"
     CDS             4028..>4354
                     /locus_tag="VFC15_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10046.1"
                     /translation="MATAILAPLEKGFAMRPINVVLAHHDAVAADRLAASLGKQFRNL
                     AVAKSAGEIAPAVARLRAPFAVVDLELVGFSELKALCSKFPSTAFVCIHRLADDRMWS
                     DALAMGA"
BASE COUNT          945 a         1257 c         1270 g          882 t
ORIGIN      
        1 cgaccggcaa ggtcgccttg atggtttgcg acggaatgtc gaactgcgct acggatgcac
       61 ccgaagtcga gccgcactca actccgcaac tgatcacata ggcggtattg ctgtcggcgc
      121 tgaagaacgc attgaccggc cgggcgaaac caggaatctc cactgcgctg accgaactgg
      181 cggtggtatt gatgaggaaa accgagtccg aatcgtccgc gaacgcgagc agatattgcc
      241 cgctgggact gagcgcaatg taccggactg agggcacgct taagttcgcc gtgattgcga
      301 aagaggcgac atcgatgacc tccacggcgc cgggacgtaa accggcgatg ggtatgttcc
      361 gcactggtgc gtacacttta ctagcgtccg gggaaaaaag cgccatctgc gcaaaatcag
      421 gaagagtcac gctgccactt accgcctcag tagcgttatc cacgaagtcg agtacatttc
      481 cactctggtc ataaaccagc gtggtggcca ggccggggct taccacttcg aacgtcaacg
      541 aggttccgac cgatatggtt actggaacac cgggtatgat ctggccagaa gagttcgtcg
      601 tctgcgcggt gaagggcgta gtgtcgtttt gcgtatccat tatttgcaag ttacccgaat
      661 aggtattgct gatgaaagcg cgatgcgaca ccttgctggg cggcgcagtt gaactcccgc
      721 tcccgccgca agacgccatg aaaaaaacga ttagcaaggt gaggaggaga actggaaccc
      781 gcttcaagcg tttaccccgg acatcatgat gaattgagat gatatgttgg aatccgctga
      841 attatagcag agcgcggcgg aacactcggg tgcgcgggcg atggcgatgg cgaatttccg
      901 cggtttgcga taaagttcaa tcaagcttgg catttcagcc tccaacatat ccatgactgc
      961 ggacctcatt tcgcgattgc agaagggcgc tgtcttatgc gatggcgcca tgggtacgtt
     1021 gctgtactcc aagggtattt tcatcaaccg ctgctacgac gagctcaacg tctcgcagcc
     1081 cgacctgatc cgcggcatcc atcaggaata cctccaggcc ggggcagaca tcattgaaac
     1141 aaacaccttt ggcgcaaatt cgtttcgttt gtcccggcac ggatgtgtgg acaagttggc
     1201 cgatgtcaat cgccaaggcg cccgtctggc acgggaggcg gcgaagagtt tcgatgccct
     1261 ggtcgctggc gccgtcggac ctctgggagt gcgtatcgag ccgctgggca aggtttcgct
     1321 cgacgaggca cgggaggcct ttcacgagca gatcgcagcc ctcaacgaag aaggcgtgga
     1381 cttgctcatc ctggagacct tcggctacct ggaggaactg caccaggcga ttctagccgc
     1441 tcgtgaagtg aatcccaaat tgcccatcgt ggcccaggtc acgatcgacg aagatggcaa
     1501 ttgcctggat ggatcggccc ccgagcacta tggtccgcaa atcgaagcct ggggcgccga
     1561 tgtcgtggga tgcaactgta gcgtcggtcc ggtggctatg ctggacgcga tcgagcgggt
     1621 acgcgccgtc accaccgcgc cactctcggc ccaacccaac gccggcatgc cgcgctcagt
     1681 ggaaggccgc aacatctatc tctgctcgcc ggagtacatg gcggagtttg cccgaaggtt
     1741 tgtggatgtc ggtgtgcgcc tcatcggtgg ttgctgcggc accacgcccg accacatcca
     1801 tgtcatgaag gcgttcctgc gcgcggtctc cggcggcacc ctggcgcgca agccggctgc
     1861 gcttgccaag gcccagccgg ttgaagtgcc gccgctccga gaacgttcgc aactaggacg
     1921 caagatcgcc gacggcgagt ttgtcaccat ggtggagatt gttccgccga gaggtaccga
     1981 tggaagccgg gagatcgaag gagcgcgctt cctgaaatcg gttggcgtgg acggcgtcaa
     2041 cattcctgac agcccgcgcg cctcggcgcg tatgagcaac caggcgctct gcttgctggt
     2101 gcagcagcag gtcgggatcg agccgattct gcactacacc tgccgtgacc gcaacgtgct
     2161 ggggattcag tcggacttgc tgggcgcaag cgcagtcggc atccgcaacc tgatctgcat
     2221 caccggcgat cccccgaaga tgggcgacta ccccgacgcg acggcggtct tcgatgtgga
     2281 cgccatcggc ttggtcaaca ttgtccacaa cctgaatcgc gggctggaca tcggcggcaa
     2341 tcccatcggc ggctcgacca agttcgtaat tgccgtgggc gccaatcccg gcagccccaa
     2401 cattgacgag gaaatccgcc gcttccaatt caaggttgag gccggcgcgg agttggccgt
     2461 cacccagccc gtgttcgata ttcggctgct ggaagagttc ctgcgcaaaa tcgagcactg
     2521 ccgcataccg gtggtggcgg gcatctggcc gctcaccagc acccgcaatg cggagttcat
     2581 gaagaatgag ctgcgggttt ccatcccgga cgagatcttc acccgcatgg cgaaggcgac
     2641 caccgtcgac gccgcccgcg ccgaaggcgt ggccattgcc cgcgacatgc tcgccgccgt
     2701 ccgccacatg gtgcagggcg cacagattgc cgcgcctttc ggacgctact cctgtgccgt
     2761 ggacgtgctg gaggcactgg gaacgggaaa acgcaccgcg gtgtaacggg cgagcgatct
     2821 cgctctctca tgtctgcccg aatccaaccc cgcaggaggg cggtcatcct cagtcagcgc
     2881 ggaggagcat tggggttgta atctacgccc tactctccga tttccgcaat ccgcttcgct
     2941 gcaagcgctg ccccttccaa cacgatttgg cgaagctcaa gtaaacttat ctcggcaata
     3001 ggggaatatt cgtgaacgtt ggagagcatc ggcattgaag accctgaacg ctgaagactg
     3061 aacgtgtaat acagtcccct gtgcacaaca tcacaccgca cgtcgtataa aagatcgacg
     3121 gcggaggtca gagatagatg gacaccgcca gcatgtcgga aagcccgcga aagccgctgt
     3181 ttactctcgg acgagcaaat ctccgtgaag aatttctgaa catgactcct ggactgaccc
     3241 tcaccttcgt aacccgccgc aatcttggcc accaactctg ccgcaataag gtaaaagagt
     3301 atttggaatg caggtctgcc gcgcgctact tcatcgatct tgtcggcaag ccataccatt
     3361 cgacccgctt gatgaaaaac aattttggca tctttcttcg agtcggggag gcttcgaata
     3421 gtctcgtaga acttggtact ggaatccaac gaaccgaaca cctcagcgta gagttgcgga
     3481 aaatcaatct cggcaatgtg gactggcgct tcaggaatca tgaaaacagg atacctcttc
     3541 cactccgttg acccttgtga aatacaaggc acccccggcc gtcctgcgcc gcggggacga
     3601 gatggcgttt ctactgggaa aatatttccc gccggagttc gattgactcc aaatttttac
     3661 attcaccgaa accgttctgc actagaatta gtaacggagt ttgagcttgc ctaaattcga
     3721 agagtctctc caacggctgg aaaaaatcgt gactgaactt gagaaggggg acgtctctct
     3781 cgatcacgcg ctggagcttt ttgatgaggg catgaagctt tccagttcct gccgcaagga
     3841 actggaggaa gccgaaggca aagtggaaat cctgctgaag cgaaatggaa agctacatcc
     3901 ggaagccttc gaacctgcga gccaaccggg cgacactgca aaatagctgc cgcccacgat
     3961 tgcctgagtt atgtaccgcg ccgcggagtg cggggtggtc gtttgttcac acaagatggt
     4021 tcattggatg gcaacagcta tccttgcgcc actcgaaaaa gggtttgcca tgcggcctat
     4081 taatgttgtt ctcgctcacc acgatgcagt tgcggccgac cgcctggctg catctctggg
     4141 taaacagttc cgcaacctag ccgtcgcaaa atctgccgga gaaattgcac ctgcggtggc
     4201 gcgtcttcgc gccccattcg cggtcgtaga cctggaactg gttggcttct ccgaactgaa
     4261 ggccctctgc tcgaaatttc cttccaccgc tttcgtatgc atccatcgtc tagcggacga
     4321 tcgcatgtgg tccgatgctt tggctatggg cgcg
//
LOCUS       DASRKK010000345         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1004002, whole genome shotgun sequence.
ACCESSION   DASRKK010000345 DASRKK010000000
VERSION     DASRKK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004002"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..2034
                     /locus_tag="VFC15_07540"
     CDS             343..2034
                     /locus_tag="VFC15_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM10047.1"
                     /translation="MFVRTQTNGSRTYLLLVDNVWADGKVKQRVLARLGRLDELLASG
                     RFDALVQSLARFSDKLCLLGAQARADSLATRSTKIGPALIFQRLWQSCSIDQALTALL
                     QGRRFEFAVERAVFLAVLHRLFAPGSDRAAEKWKADYAIEGADELDLHHLYRAMAWLG
                     EVLPQDQQGGATPFAPRTNKDLIEEALFAQRRDLFSQLDLVFFDTTSIYFHGQGGETI
                     GHRGHSKDHRPDLKQMVVGMVLDHQGNPVCSELWPGNTADVKSLVPLVERLRTRFGIG
                     SVCIVADRGMISAATQAEVEKRGWKYILGVRMRSSTEAKAVVARAGRYAQVHPKSDDP
                     KDPSPLKVKEVWVEDQRRYVVCVNEAQATKDRYDREAVVASLRDALRHGDKSLVGNKG
                     YRKFLQAGRKQFTVDEDKIQEEARYDGKWVLTTNTDLAASEIALKYKQLWMVEEVFRS
                     MKSLLDTRPIFHQRDETIRGHVFCSFLALLLRKDLQDRLTAKGCDAEWADVLRDLDNL
                     VEMEVAINGKGYVFRGQTTGVAGRVFQACGVALPPLMRPSSPAKPLITPEEKACH"
     gene            complement(2132..>2320)
                     /locus_tag="VFC15_07545"
     CDS             complement(2132..>2320)
                     /locus_tag="VFC15_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10048.1"
                     /translation="VTKGMDFKDEDRVDPEDFNRILWKGLMGRKPYPEATAGTDLREN
                     REELLARYRRSLKEKAAR"
BASE COUNT          471 a          688 c          682 g          479 t
ORIGIN      
        1 tgcgcaagca cgccagaagc tgggagtgcg gaatcgaatc gaaatagccc ttcaagtccg
       61 cgtcatacac gacctgatac ccggcctgca aatgaccgcg tatttccgcc agcgcctggt
      121 gggctgaccg ccccggtcga aacccgtacg agcaatccag gaaatctgcc tcaaactcat
      181 cttctacact ttgcgattca aagtcgctgt aactgcctga ttccacacat tgcgcggtcg
      241 ttctgcgcga aaacctagtg tcacgtgttc caaactaact ctgattctat ccttgtttct
      301 tgcgttataa ggtgctaatc atagtgacac aggagcgtaa ctttgttcgt ccgcacgcaa
      361 accaatgggt cccgaaccta tctactcctc gtcgataacg tctgggctga cggcaaggtg
      421 aaacagcgcg tcctggcccg cctcggccgc ctcgacgaac tcctcgccag cggccggttc
      481 gacgccctgg tacagtctct cgcgcgcttc tccgataagc tgtgcctcct cggcgcgcag
      541 gcccgcgccg acagcctcgc cacgcgcagc accaagattg gaccggcgct catctttcag
      601 aggctctggc agtcctgttc catcgaccag gccttgacgg ctttgttgca aggccgccgc
      661 ttcgaatttg ccgtagaacg ggctgttttc ctcgccgtac tgcatcgctt gttcgccccc
      721 ggcagcgacc gtgccgccga gaagtggaag gcagactacg ccatcgaggg cgccgacgag
      781 ttggacttac atcacctgta ccgggccatg gcttggctgg gagaggtgct gccacaagac
      841 cagcaaggtg gcgctacgcc gtttgctccc cgcaccaaca aggacctcat cgaagaggct
      901 ctattcgccc aacgtcgcga tctgttctcg caactggatc tggtgttctt cgacactact
      961 tcgatctact tccacggaca agggggcgag accatcggtc accgcggcca ctccaaagac
     1021 catcgtccgg atctcaagca gatggtggtg ggtatggtgc tggatcacca gggcaacccg
     1081 gtgtgcagtg agctctggcc ggggaacacg gccgacgtca agagcctggt gccgctggtg
     1141 gagcggctcc gcacgcggtt tggcatcggc tcggtttgta tcgtggcgga tcgggggatg
     1201 atcagcgccg ccacccaggc ggaagtcgag aagcggggct ggaagtatat cttgggtgtg
     1261 cgcatgcgga gttccacgga agccaaggcg gtggtagcgc gcgccggacg ctacgcccaa
     1321 gtgcatccca agagtgacga ccccaaggat ccctcaccgc tcaaggtgaa agaggtgtgg
     1381 gtggaagacc agcggcggta tgtggtctgt gtgaatgagg cgcaggccac caaggatcgc
     1441 tatgatcggg aggccgtggt ggcctcgctg cgggatgcgt tgcgtcacgg cgataaatcg
     1501 ctggtgggca acaaggggta tcgcaagttc ctgcaagcgg gccgcaaaca gttcaccgtg
     1561 gatgaggaca agatccagga ggaggcgcgc tacgacggca agtgggtgct caccaccaac
     1621 accgatctgg ccgccagtga gatcgccctg aaatacaagc agttgtggat ggtggaggaa
     1681 gtgttccgct ccatgaaatc gctgctggac acccggccca tttttcacca acgcgatgag
     1741 accatacgcg ggcacgtctt ctgctcgttc ctggcgctgc tgttacgcaa ggacctgcaa
     1801 gatcgcctca cggccaaggg ctgtgatgcg gaatgggcag atgtcctccg cgacctggat
     1861 aacttggtgg aaatggaagt tgccatcaat ggtaaaggct acgtgttccg cggccaaacc
     1921 acaggcgtag ccggcagagt gttccaggct tgcggcgtcg ctctgcctcc actgatgcgt
     1981 ccatcctcgc ctgcgaaacc gctgatcacg ccggaggaaa aagcgtgtca ctaggtcgtt
     2041 tcaaaaattg caaatccttg aaatcaaagt tcttgcgatt tcatggtgtt gaagatgagt
     2101 taaacggtaa atttcttatt tccggactcc gctaccgcgc agctttctcc ttcagagacc
     2161 gccggtatcg cgcgaggagt tcctcacggt tctcgcgcag gtccgttccg gccgtcgcct
     2221 cgggataagg cttgcggccc atcagccctt tccagagaat gcggttgaag tcttccggat
     2281 cgacgcggtc ttcatccttg aaatccattc cctttgtgac
//
LOCUS       DASRKK010000346         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1199211, whole genome shotgun sequence.
ACCESSION   DASRKK010000346 DASRKK010000000
VERSION     DASRKK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199211"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFC15_07550"
     CDS             complement(<1..307)
                     /locus_tag="VFC15_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10049.1"
                     /translation="MWYGSTKNLLESIVRGLNTEPPQNDAEWQTQTDLVQACLAEMAE
                     LAEPTVNPANGFTSRYVHHPVADKLNRAMPHVRLMLTAMRDRDRTTALAHGEVTLRRL
                     "
     gene            complement(481..711)
                     /locus_tag="VFC15_07555"
     CDS             complement(481..711)
                     /locus_tag="VFC15_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10050.1"
                     /translation="MGGKEGTRKGGEESWRVGIATEGGGEGFRSHSDRRSVEAGLQVG
                     RSGEYQAGGESTQKSWDGAGTAGPVRQSGSGR"
     gene            complement(747..1256)
                     /locus_tag="VFC15_07560"
     CDS             complement(747..1256)
                     /locus_tag="VFC15_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10051.1"
                     /translation="MPTHKLTPEIITAAIVGFEAQKTRIDDQIAELRAMLSGGTKPTA
                     ATTPGPPTSQRRKFSAAARRRMKEAQQRRWAKIRGESEPPALVKSEASKPKRKLSKAG
                     RAAIVGAIKARWDRVRAEAGKAAPAVAKKAAARKSVARRKLSPAGKAALVANLAKARA
                     AKAAKKGTK"
     gene            2020..2466
                     /locus_tag="VFC15_07565"
     CDS             2020..2466
                     /locus_tag="VFC15_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZM10052.1"
                     /translation="MNDTNFGLLKATLETKLATIATGSDKRADIVIQQSADDLDQTQF
                     ATERDLTVSLLNRDSQMSRRVKGALRRMEDGTYGTCLACEEPISVKRLQAVPWAELCI
                     HCQERSDRAAAGAPGAGHAEGVELGVRWEQFPAQEKVSENGGADRM"
     gene            complement(2873..3256)
                     /locus_tag="VFC15_07570"
     CDS             complement(2873..3256)
                     /locus_tag="VFC15_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10053.1"
                     /translation="MTITYPDGTVLEAIVLAHEENEIRAIATGCNDALAFTRIHGTWI
                     SEEIEPVAIEFAWQRRGTSPTPSLAACICPKTLAARLIQSLFRGCEPVVAEPETLCVF
                     SPEGKRVPISISELPQEVGRGSRCH"
     gene            complement(3414..3722)
                     /locus_tag="VFC15_07575"
     CDS             complement(3414..3722)
                     /locus_tag="VFC15_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10054.1"
                     /translation="MWYGNTKNLLESIVRGLKTEAPQNDAEWQAQTELVQVCLTEMVE
                     MSEPAANPSMGATSRYVHRPVADKLNRAMPHVRSMLTAMRDRDRTTALAHGEVTLRRL
                     "
     gene            complement(4300..4659)
                     /locus_tag="VFC15_07580"
     CDS             complement(4300..4659)
                     /locus_tag="VFC15_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10055.1"
                     /translation="MTITYPNGTVLEAIVLSHEENEIRAIVAGCDDVLAFTRIHGTWI
                     SEEIEPVAIEFAWQRRGTSPSTSEDDCVCSKKLAARLIESLLSGCERGEAGEDTLYVF
                     SPEGSRVAIHRTELRPL"
BASE COUNT          963 a         1574 c         1532 g         1141 t
ORIGIN      
        1 agagccgccg gagggtcact tcgccgtgcg ccagggccgt agtacggtct cgatcccgca
       61 tggctgtcaa catcaaccga acgtgcggca tcgctcgatt cagcttatcc gcaaccggat
      121 ggtgaacata tcgtgacgtg aacccgttcg ccggattgac agttggctcc gccagttccg
      181 ccatctccgc aaggcaggct tgaacaaggt cggtttgagt ttgccactcg gcgtcgtttt
      241 gaggaggttc ggtgttcaac ccccggacga tgctttccag taggttcttc gtgctgccgt
      301 accacattgg agaattctct gccttcctga caacgtcgcc ggattggaac cgtacatccg
      361 atatgcaatc gccgtgatgc acacgtgccg gaccaccgcc ccagcctccg cattgggctg
      421 aatgccggtc aattccaggt atgatccgtc cgggaagctg accaggggag cgcaagagca
      481 tcatcgcccg gaaccgctct ggcgaacagg tccagcggta cccgctccgt cccaggactt
      541 ctgggtcgat tcaccgcccg cctggtactc gcccgaccgt ccgacttgca gtccagcctc
      601 tactgagcgg cggtcgctgt ggcttcggaa gccttcgccg ccaccttccg ttgcgatccc
      661 aacccgccag ctttcttcgc cgcccttgcg ggtaccctct ttgccgccca acgtttcttc
      721 gtggagcgac gattcccgcc tcgctgttac ttcgtacctt tcttcgcagc tttggccgcg
      781 cgtgccttcg ccagatttgc caccagtgcg gccttccctg cgggggacag tttccgcctt
      841 gctacggact tcctcgcagc ggctttcttg gcgacggctg gtgccgcctt cccagcttcg
      901 gcccggaccc gatcccaacg agctttgatt gcgccgacga ttgcggctct gccagctttg
      961 ctcagttttc gtttcggctt cgacgcttcc gacttgacca gcgctggcgg ctcagattcg
     1021 ccccggatct tggcccatcg gcgctgttgg gcttccttca tccgccgtcg agccgcagcg
     1081 gagaatttcc tgcgctggct ggtgggcggt cccggcgtgg tggcggcggt tggtttagtg
     1141 ccacccgaca gcatggcgcg cagttcggcg atctgatcgt ctatgcgtgt cttctgcgct
     1201 tcaaaaccta cgatggcggc agtgatgatt tcgggagtta gtttgtgggt gggcataaag
     1261 cattagtatg ccacccttca ccccacgtgt tggcaatccg actccgcaac agcctggtca
     1321 tcgccagcgc ccgccctgcg cagcagtcgg acggcccgcc cgcgcaaacc cgagccactg
     1381 caggggggct tgcggatggt gggcgggtag cttcgttgcg ctggcagcat gcatcgctga
     1441 tccggcatga gacccggagc ggagcctacc cggcatccga gtacctgata ccaatgcggt
     1501 gcaggaattt tcggtcatac gccgtatcga ctgtcggctt tgctttcggc agattcgctg
     1561 atgccgcagt gagacaatct cgccaacaat ccgaaagagg tttcacagaa cagatgcttc
     1621 tgcggcaccg tgggcatcgc cagtgaatca cgagttcatg ctctgacgag aggccgaccg
     1681 ctgatatctg cttcggcact ccaccacact cgcacgacaa cggcaatgtg cggtattcca
     1741 tttcagacac tccgttttcc gtctatcagt ttcgcaacca gcgctcctga ccgacgtggg
     1801 cggtgaggtt ggtgctcact tgaatctttt cagcagtccg acgccacgac aatagcagca
     1861 atggttagtg gtagccagat atccgatcaa aacaggaagc ggggatggca aaagtgatcc
     1921 gagatcgcct tccctcgggt agactcccca tatctaaaga taaccttggt tccggagggc
     1981 ctcctggatc atagtgcatt aacagtaggg ggtggtttca tgaacgacac gaactttggc
     2041 ctgctgaaag ctaccctgga aaccaaactc gcgacaattg cgaccggctc ggacaagcgc
     2101 gcggacattg tgatccagca aagtgcggac gatctggatc agactcagtt cgccaccgag
     2161 cgagacctca cggtgtcgct gctcaatcgc gatagccaga tgtcacgccg cgtgaagggc
     2221 gcgcttcggc gcatggaaga cggcacatat gggacctgtc tcgcctgcga ggagccgatc
     2281 agcgtgaagc gattgcaggc tgtgccatgg gcggagttgt gcatacactg ccaggagaga
     2341 tcggaccgag cggcggcagg agcccctggc gctggtcatg cagaaggcgt ggagttgggg
     2401 gtccgatggg aacagtttcc cgcgcaagag aaggtcagcg agaacggtgg cgctgacagg
     2461 atgtgagggc tgcgctcagc cccacccacg gcgcgttcct tttggcctcg gttacgtaga
     2521 ggtgcgccgc gtcgaaggtg cggtggcctg gacatccgag gctcccaaat ggcaatctag
     2581 ctgagacctt tgaccctggt gtagtagtca gggtcgccat accgtccctc gaaccaagta
     2641 tcgtcgtccc acttgggatt cccgctttcc cgatggcggg cgggaggtgt gcttttgggc
     2701 ggagcaacag gctttttcaa cgaatgttca agaagagatt cttgcgttgg cgtaagcagc
     2761 agcagttctt gggtttgcgc tcgctcgccc ccaggacgtt ccggccctcg cagatccaca
     2821 gggtctttcc gtttcttcaa ccagtggtta agaaacgagc tttgcgctga cgttagtggc
     2881 atcggctccc gcgtcctact tcctggggca gctcagaaat gctgattgga acgcgctttc
     2941 cctccgggct gaatacacag agcgtctccg gctccgccac cacgggctca cagccacgga
     3001 acagcgactg gatcaggcgg gcggccagtg tcttgggaca aatgcaggcc gccagggagg
     3061 gcgtgggaga ggtccctcgg cgctgccacg caaactcaat ggcaactggc tcgatctctt
     3121 ccgaaatcca cgtgccgtga atgcgagtga atgccagcgc gtcgttgcaa ccggtggcaa
     3181 tggcgcggat ttcgttttct tcgtgggcga gcacgattgc ttcgaggaca gttccgtctg
     3241 gataagtaat ggtcacaatt gcattgtgcg ccactgggta agcgcaaacc agtccttggc
     3301 gctccaggca gggcacagga ccataccaaa cgttaacttt tctgactgaa attaacgaga
     3361 ccggcgtcgt gcggttgccc aacgtggcga tcccggcgtt gcaccgatcc ggctcagagc
     3421 cgccggaggg tcacttcgcc gtgcgccaga gccgttgtac ggtcacggtc ccgcatggct
     3481 gtcaacatgg atcgaacatg cggcatcgct cgattcaact tgtccgcaac cggacggtga
     3541 acgtatcgtg acgtggcccc catcgaggga ttggcggcag gttccgacat ctccaccatc
     3601 tccgtcagac agacttgaac gagttcagtt tgagcttgcc actcggcatc gttttgagga
     3661 gcttcggtct tcaacccccg aacgatgctt tcgagtaagt ttttcgtgtt gccataccac
     3721 attggaggat tctttgcttt ctgttctagg ctctttgcaa tcagccgtac tgccgctctt
     3781 tgttaaaccc ccacaggggc aggatcatga ccgacgacaa gcctatcaaa cgaatcccaa
     3841 tcaaggatat catctccgag acggcgcaca tgaactgaca acgtcgccgg atatggaacc
     3901 gtgcatccga tttgcaacgg cggtgctgca cccgtgactc tgtgcggtcc cgcctccgtt
     3961 cgctgatgaa ccagagcgtt gctcttgagc acctgaactc gggaccgcaa gaacatcatc
     4021 gtccggaacc gctctgctga acaggtccag cggtacccgc tccgtcctag gacttttgcg
     4081 tcgattcacc gaggcctggt actcgcccga ccgcgatcac caacggaaca gttccgagcg
     4141 attctgcacg cggcgaggct ccgatacccg gccccgtcgc cgcccggcaa aaattttcgg
     4201 cctccagtag cggatcggtt cgatgaataa gacgttggtt tcggcgtgga catcattggt
     4261 cctgccccgt ctaccgtagc tcttcggcga ttgcggtctt tacaaaggtc gcagttcagt
     4321 tcggtggatg gcgacccggc tgccttccgg gctgaaaaca tagagcgtgt cttcacccgc
     4381 ctcaccccgc tcacagccac tgagcagcga ctcgattagg cgggctgcca gtttcttgga
     4441 gcaaacgcaa tcgtcctcgg aggtagaggg agatgtgccg cgacgctgcc aggcgaactc
     4501 gatggcgacc ggctcgatct cttccgaaat ccaggtgccg tgaatgcgag taaaagccag
     4561 cacgtcgtcg caaccggcga caatggcgcg gatttcgttt tcttcatgcg agagcacgat
     4621 tgcttcgagg acagttccgt tggggtaggt gatggtcaca attgcattgt gcgcccaaga
     4681 ccaagcgcga accagtcctt ggcgctccat agagggcaca ggaccgtact aacgttagct
     4741 ttatctctga agaggcgggg aaccggcctt ggtacgacgg cgacggttgg cgtctagctc
     4801 gcaggcggcg tagcaggatg atgtggcgcg ggcgtgatcg ctgattgagc gggattgggt
     4861 caccgctccg gctgcgcttg tgacagttgc cggttaaaaa gcctttagtt ctcctacttc
     4921 ttagattttt accctcgatc tgtcacaacc aagctgcgcc aagtgatgca catgatttgg
     4981 cagtgctact cggtcgccgt cggaacagca ggcgcggcgg ttttctgcgg tcgctcggcg
     5041 gcaaccttct tcgcggcctt ccgtgaagcc ttcaccggcg ctttcttcgc aacggtttct
     5101 tggcggctgg tgtctgctgg gccttggccg ccgccttcgc tgccgccttc ttcgcggccc
     5161 accgtttctt caaggcggcg acgattgcgg ctctacctgc cttgctcagt
//
LOCUS       DASRKK010000347         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_26532, whole genome shotgun sequence.
ACCESSION   DASRKK010000347 DASRKK010000000
VERSION     DASRKK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26532"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1201)
                     /gene="istB"
                     /locus_tag="VFC15_07585"
     CDS             complement(386..1201)
                     /gene="istB"
                     /locus_tag="VFC15_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM10056.1"
                     /translation="MKPATEALEHASVKQYCKALRVPVIGANFVPLAEQAVKEKRNHI
                     GYLEALLAMETEERDRHAVQTRLHDARLPRMKTLEEFDFNQAPHISAAKIRELAEGGY
                     IERAEPVVLMGECGTGKTHLATGLCVEACRQKRRVRFTTAANLVNELVEAQQQGGVRR
                     VLARWMRYDLIALDEVGYVPLAEIGAEFLFQVIAERAERASLIVTTNLPFSEWTQVFP
                     NPRLCKALLDRITDRAHIIETGTDSYRFRRTLEVRKKKGTRGGDAVVTASATT"
     gene            complement(1198..2721)
                     /gene="istA"
                     /locus_tag="VFC15_07590"
     CDS             complement(1198..2721)
                     /gene="istA"
                     /locus_tag="VFC15_07590"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM10057.1"
                     /translation="MELFEAIRREYVHGTGTIQGVSRKLGVHRRMVRQALVSAVPPER
                     KQPVRSHPSLGPVMDFIDGILIADERAPKKQRHTARRIWQRIRQERPEASVGEATVRG
                     YVQRRKQELGQVRRETFIPQVYDWGVEAQVDWYEAMAEIGGEMRKVYGFSMRSMASGG
                     AFHVAYYHATQQAFLEAHELAFSYFGGVFRLLRYDNLKSAVKKILRGHQREETERLIC
                     FRSHWGFQTDFCNPARGNEKGGVEGEVGYFRRNHLVPVPRVQDLAELNALLLQGCQQD
                     QQRSIAGKMMKVGEAMEVERQHLLPQATEGFELAETSFPKVDGKSCVKVRTNWYSTPV
                     KPGIHVQARLLPAYVEVWQERKLVARHERSFARYEQVLDLEHYLDVLLRKPGALAGSR
                     PLQQCRERGHWPESFDQLWQSLQRREGKQAGTRSMIELLQQGSQHGWEKLKQAVQQAL
                     DIGCTDAAAVRHLLVFGELAHAPVERLALGGLERYERPLPVLSDYDLLLDEVAEVAR"
     gene            complement(2837..>3173)
                     /locus_tag="VFC15_07595"
     CDS             complement(2837..>3173)
                     /locus_tag="VFC15_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZM10058.1"
                     /translation="LDPMQRARRIILIDATLDNEPPGTVRRLTPRFSSDYPRTLTAHD
                     IGLKDLLDTFYLLGHPVDVVLFAISIASLQEIGLDLCPEIAERVPEIAQLVLHELMSD
                     LHLEGQGAL"
BASE COUNT          598 a         1049 c          867 g          659 t
ORIGIN      
        1 ggactgaaaa gcaggggtta ggggctagta gctgtcagcc atcagctctc agcattcagc
       61 ccctcgatac ttagcaatag cattcattcg cgagatttcg atcgtcatcc tgacttcggt
      121 agtgcggacc ttgcccgcgt gtcgcgaccc cgtctcggtt tgctacagca ggaaggaagc
      181 acgcaaagat cagccccgac tggggcgaaa gagcttagcc cagcgcttta cctgaattat
      241 tcatgaggat gcttttgaca acctgacttg gcccacctgt gctcatctca tttggcccac
      301 ccgcggttaa tagttctcta ctcggcaacg ggtgagcgtc gttgctgccg gagcggagac
      361 ctgcgctgtc cgcaggacag cgcgtttagg tcgtagcgga ggcagtaacg acggcgtcgc
      421 cgcctcgggt ccctttcttc ttgcgcacct ccagggttcg tcggaatcga tacgagtcag
      481 tgccagtctc aatgatgtgg gcgcgatcgg tgattcggtc cagcagcgcc ttgcacagtc
      541 ttgggttcgg aaacacctgt gtccactccg agaacggcag gttcgtggtc acgatcagcg
      601 aggcgcgttc tgcccgttcg gcgatgacct ggaacaggaa ctcagcccca atctcggcca
      661 gcggaacata gccgacctca tccagagcga tcaggtcgta gcgcatccat cgtgccagca
      721 cccggcgcac cccgccctgt tgttgggctt cgaccagctc gttcaccaag ttggcagcgg
      781 tggtgaaacg cacgcgccgt ttttggcggc aggcctcgac acacaacccc gtggccaagt
      841 gggtcttgcc cgtgccacat tcgcccatca gcaccaccgg ttcggcgcgt tcgatgtagc
      901 cgccctcggc taactcgcgg atcttggcag ctgagatgtg tggggcctgg ttgaagtcga
      961 actcttccag cgtcttcatg cgtggcagcc gtgcatcgtg aaggcgcgtc tgcaccgcat
     1021 gccggtcgcg ttcttcggtt tccatcgcca gcagtgcttc cagatagccg atatggttgc
     1081 gcttctcctt caccgcctgt tccgccaggg ggacgaagtt agcgccgatc accggaaccc
     1141 gtagggcctt gcaatactgc tttacgctgg catgttccag agcctccgtg gcgggcttca
     1201 tcgcgccacc tccgccactt cgtctagcag aaggtcgtag tcgctcagca ccggcagcgg
     1261 gcgctcgtag cgttccagtc cgcctagagc aaggcgctcc accggagcgt gagccagttc
     1321 tccaaagacc agaagatgcc gtaccgctgc cgcgtccgta cagccgatat ccagggcttg
     1381 ctggaccgct tgcttcagct tctcccaccc atgttggctg ccttgctgta acaactcgat
     1441 catgcttcgg gtcccggcct gcttgccctc gcggcgttgc agactctgcc acaactgatc
     1501 gaaactctcc ggccagtgcc cgcgttcgcg gcactgctgc aagggccgcg atcctgccag
     1561 cgcacccggc ttgcgtagca acacgtcgag atagtgctcc agatccagca cctgctcata
     1621 acgagcgaag ctgcgctcat gtcgcgccac cagcttccgc tcctgccaga cctcaacata
     1681 ggctggaagc agtcgcgctt gcacatgtat ccccggcttc accggcgtcg agtaccagtt
     1741 cgtgcgcacc ttgacgcagc tcttgccatc cactttcggg aaactagtct ccgccagctc
     1801 gaagccttcc gtcgcctgcg gcagcaggtg ttggcgctcg acttccatcg cctcgcctac
     1861 cttcatcatc ttgcctgcga tactccgctg ctggtcttgc tggcagccct gcagcaacaa
     1921 cgcgttcaac tccgccaagt cctgcacccg aggaaccggt accaggtggt tgcgccgaaa
     1981 ataacccact tcgccttcga ccccaccctt ctcgttgccc cgcgccgggt tgcagaagtc
     2041 ggtctgaaag ccccagtgcg aacgaaaaca gatcagtcgc tccgtctctt cccgctggtg
     2101 accgcgcagg atcttcttca ccgcgctctt caggttgtcg tagcgcagca accggaagac
     2161 accgccgaag tagctaaaag ccaactcgtg ggcttccaga aaggcctgct gtgtggcgtg
     2221 gtaataagcc acatgaaacg cgcccccgct ggccatcgac cgcatcgaga agccatacac
     2281 tttgcgcatc tcaccgccaa tctcggccat cgcctcgtac caatccacct gtgcttccac
     2341 gccccagtcg tagacctggg gtatgaaagt ctcccgccgc acctgcccca gttcctgttt
     2401 gcgacgctgc acgtaaccgc gcaccgtggc ctccccgaca ctcgcctccg ggcgctcctg
     2461 cctaatccgc tgccagatcc gccgtgccgt gtgccgctgc ttcttcgggg cccgctcatc
     2521 ggcgatcaaa atcccgtcga tgaaatccat caccggccct aaactcggat ggctccgcac
     2581 tggctgcttc cgctctggcg gaaccgcact taccaaggcc tgccgcacca tccgccgatg
     2641 aacacccagc ttcctggata ctccctggat cgtcccggtt ccgtggacat actcccgtct
     2701 gatcgcctca aataactcca ccttcgttct cctgtccatc ggcctccctg tcggctccgc
     2761 cgacagagta gccaattagt gggtgagggt gggccaaatc agacgcgcac aagtgggcca
     2821 gatcagagta gcgaaatcaa agggcgccct ggccttccaa atggaggtca gacattaact
     2881 cgtgcaatac cagttgcgct atctcgggaa ctcgttctgc gatttccgga cacaagtcca
     2941 ggccaatttc ctggagagaa gcgattgaga ttgcaaacag gaccacatct acaggatggc
     3001 ctagaagata gaacgtatcg agcaggtctt taagacctat gtcatgggcg gtgagagtgc
     3061 gggggtagtc ggaagaaaac ctgggcgtca atcgacgtac ggtgccgggc ggctcgttgt
     3121 ccagagtcgc atcaatgagg atgatcctgc gcgccctttg catcggatcc agg
//
LOCUS       DASRKK010000348         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1395257, whole genome shotgun sequence.
ACCESSION   DASRKK010000348 DASRKK010000000
VERSION     DASRKK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395257"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFC15_07600"
     CDS             <1..432
                     /locus_tag="VFC15_07600"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZM10059.1"
                     /translation="VTLVVDDFVQPNGLCFSPDEKKLYVIDSGITHGGPAHIRVFDVD
                     IATGKVINGKVFAEGFAPGFTDGMRCDVEGNVWCSMGWGDPKEDGVRCYSPEGDLLGK
                     IHLPETCANLVFGGPLHNRLYMCASTSVYACYVGVQGAAKP"
     gene            complement(530..1162)
                     /locus_tag="VFC15_07605"
     CDS             complement(530..1162)
                     /locus_tag="VFC15_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10060.1"
                     /translation="MIELSHIYGRLALAFGALLIPMALVCAPTHADGTPVRVRGSVVS
                     LDGSKLVVHAKDGNDVTVSLADNFAALAVVKSSMADIKEGTFIGTATVTQPDSTLRSV
                     EVVVFPDSLRGTAEGHYPWDLGSSSMMTNATVANAVKGVDGQTVTVTYKGGEKKIDIP
                     ANVPVVAVVPASKDDIAPGAIVFVPTERQSDGSLHGGAVLFGKDGVIPPM"
     gene            complement(1464..1610)
                     /locus_tag="VFC15_07610"
     CDS             complement(1464..1610)
                     /locus_tag="VFC15_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10061.1"
                     /translation="MENRSLHCASVRPTTQDSMKANSPMDSNAKMMKKKHKIKKGVMK
                     SDEA"
     gene            complement(1815..>2309)
                     /locus_tag="VFC15_07615"
     CDS             complement(1815..>2309)
                     /locus_tag="VFC15_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZM10062.1"
                     /translation="YELTLRSRQPDGKQATSKQSVVVALAEVESSSGAVRSRAEVPFN
                     VPGTVGANRSVLDQAVGSSQAHQPSQPPLQIAKRQDIAVSQLPHATAATPLSDGGSPS
                     AVVVPKIATTVVSRGDSLWRISRVTYGAGMRYAVVYKANQDQIRNPNRIYPGQIFVLP
                     MKER"
BASE COUNT          477 a          673 c          664 g          495 t
ORIGIN      
        1 gtcacactcg tcgtcgacga tttcgtgcag ccgaatggtc tctgcttttc gccggacgaa
       61 aagaagctat acgtcattga cagcggcatc acccatggtg gtccggctca tatccgcgtc
      121 ttcgacgtcg acatcgccac gggcaaagtg atcaacggaa aagttttcgc cgagggcttc
      181 gcacccggat ttaccgatgg catgcgctgc gacgtcgaag gtaacgtctg gtgcagcatg
      241 ggttggggcg acccaaagga ggacggggtt cgctgctata gccccgaggg cgacctcctc
      301 ggcaaaatcc atctgcccga aacctgcgct aacttggtct tcggtggacc gctgcacaat
      361 cgattataca tgtgcgcgag cacctccgtc tatgcgtgct acgtgggcgt tcagggtgcg
      421 gcgaagcctt aatctgggtc gggccggacg gcctcagttc ctggctgagg ccgtctcgcg
      481 cgattgaagg ccgtcgccgt tgagttgggc ttgcggagaa tctcagcgct tacatgggcg
      541 ggatcacgcc gtccttgccg aaaagaacag caccgccgtg caaggagcca tccgattggc
      601 gttcggttgg cacgaacacg atggcaccgg gcgcaatatc gtccttgctc gccggaacaa
      661 ccgcaacgac cggcacattg gcggggatgt caattttttt ctcgccgcct ttataggtca
      721 ccgtcactgt ttgaccatca acacccttaa cggcattagc gaccgtcgca tttgtcatca
      781 tgcttgatga gccgaggtcc caaggatagt ggccttcggc ggtcccgcgc aaactatcag
      841 ggaacacgac gacttccact gatcgcaagg tcgaatcggg ttgtgtcacg gtcgcggtgc
      901 caatgaacgt gccttcttta atatccgcca tcgacgactt cacgacggcc agcgcggcaa
      961 aattgtcggc caagctgacg gtgacgtcat taccgtcctt ggcgtgaacg acgagtttgg
     1021 acccgtcaag gctcacaacg gagccgcgca cgcgtacggg agtgccgtca gcatgagttg
     1081 gcgcgcagac tagcgccatc ggaatgagaa gcgcgccaaa agcgagcgcc agacgtccgt
     1141 aaatatgcga aagctcgatc atcgagtcct cccatgtccg cgcgacaacg atgcgcttag
     1201 agcgccacga acaaacgtat cacctatttc agctgctcga aacgggatac tggcgtctgt
     1261 ctaacaaaaa gccccgtata tcgcatagaa gcaggcctcc cgcgatcaac gtggcgataa
     1321 ggaacggaaa aatctcgcgt tggtctttag aacgcctcaa gataaaaagc cgcgccagcc
     1381 gaaggaaacg cggcatttcc acctagaaag ttacttggac attccgtcct tcttcatccc
     1441 gtcctttgac atgcatccct tcgtcaggcc tcgtcggact tcatcacgcc cttctttatc
     1501 ttatgctttt tcttcatcat cttggcattg gagtccatcg ggctattggc cttcatggaa
     1561 tcctgggtcg tcggcctgac cgaggcgcag tggagagatc gattttccat aacattcgat
     1621 ccccgtaatg tttggggcgt ctaaaccaac gtccgttctt ggggcaagag cgggacatct
     1681 gctcgtgctg agtgcttctc agtttgaccc aatgctgact tctgtggatt ctcaggcgag
     1741 ccgcgcatgg tcgagcgcga gcaaaaaagg cgcgcaacga ttcctcgtgc aggcgctgga
     1801 attgccgcca cttttcagcg ctctttcata ggaaggacaa agatctggcc gggataaatt
     1861 cgatttggat ttcgaatttg gtcctggttc gctttgtaaa cgactgcgta tcgcatgccc
     1921 gcgccgtagg tgacacggct gatgcgccag aggctgtccc cacgggatac gaccgtggtc
     1981 gcgatcttgg gcacgaccac ggcggacggt gagcctccgt ctgacagagg ggtggcggca
     2041 gtcgcgtgcg gcagctgtga aacggcaatg tcctgccgct tcgcaatttg caaaggaggc
     2101 tgggagggct ggtgcgcttg agaagaccca acggcctgat ccagcacgga acggtttgcc
     2161 cccaccgtcc ccggaacatt gaacggcacc tcggcgcgcg atcggaccgc gccggagctg
     2221 gactccacct cggctagcgc gaccaccacg ctctgtttgg atgttgcctg cttgccgtcc
     2281 ggctgtctgg acctcaacgt cagctcgta
//
LOCUS       DASRKK010000349         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_26893, whole genome shotgun sequence.
ACCESSION   DASRKK010000349 DASRKK010000000
VERSION     DASRKK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26893"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..402)
                     /locus_tag="VFC15_07620"
     CDS             complement(169..402)
                     /locus_tag="VFC15_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZM10063.1"
                     /translation="MKQVPAEKFKAHCLAVMDEVQATGEPVVVTKNGKPVVKLVPAKM
                     KDENIFGYMAGKAKIAGDIVSPVTPLEDWKALK"
     gene            complement(673..1479)
                     /locus_tag="VFC15_07625"
     CDS             complement(673..1479)
                     /locus_tag="VFC15_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZM10064.1"
                     /translation="MSDTRIVTFTTDFGLNDPFVGIMHGVVLNIHPVTTLVDVCHAVT
                     SYDVFDGAWTIAQAYRFFPPRTVHVVVVDPGVGGTRRPIIVETDDYVFVAPDNGVLSL
                     VEMREPKFTVRHITAERYFLQPVSQTFHGRDVFSPVAGWLSKGVAPAEFGPEISDYAR
                     LTLPAVERIGENSLRGVVLKVDKFGNLITNISEQEAPALFTTTPSAVSILVAGQTITR
                     VCHSYAEGGEDEFFAIVGSSGYLEIAAKQASAAEKLASGLGTPVGVMFGA"
     gene            complement(1538..3019)
                     /locus_tag="VFC15_07630"
     CDS             complement(1538..3019)
                     /locus_tag="VFC15_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10065.1"
                     /translation="MLTRARFVLAVCLALLYFSSAAYVRAQAVLAIPRDPSEVSLPAE
                     RSSSSLNVPADSGSAATASATGIVAVPASPKRETGIDWLHLAEGSFAFLTVEHVFRYA
                     TEYGTRDAFDTPFWPGYLNSVSNLHGWADGDPFLVNYVGHPMQGAVAGFIWQHNDRAY
                     RTVEFGKNSRYWKGKLRGAAFAYLYSVQFEIGPISEASIGHVQAFFPQQGFVDHVITP
                     TVGLGWTLAEDSLDRYVITALEAHNRNPYVRLLVRSGLNPSRSMANVMNGMVPWHRDN
                     RPGVFKPYPEAAAAKAALERDTARVPVNPPPGVAPFEFTMTPNFRTYLGSGAKGSCIG
                     GGSSIAFRVVPEWQIVVDVNGCKLLDLPTNLSGDSLSYMIGPRWTPSTAGRWNAHAQF
                     LVGGTKLTQERLFPEEKATVIAAAPPADQGYPLHHLYTKDWDTNGFAIAAGTGVDYKI
                     NNALAFRVASLEYSHSWTQDLNGMNYQNGLQVTTGLVLRMGTW"
BASE COUNT          685 a          945 c          901 g          682 t
ORIGIN      
        1 cgctggagaa ttgaaaccta cactggcatc aagatgcaga gcatcggctt cctcgaattt
       61 tttgggttca cctggcgcga gcgtgtggag ctgtttcgct tcttgaagtg gacgggcgaa
      121 atggaacgct atgcccgggt caaaccgaag aacccgtgaa caaccgaatc acttcagcgc
      181 tttccaatct tcgagcggtg tcactggcga gacaatgtcc ccggcgatct tcgccttccc
      241 agccatgtag ccgaaaatgt tttcgtcctt cattttggca ggcacgagtt taaccaccgg
      301 cttgccgttt ttggtgacga cgacaggttc acccgtagct tgtacttcat ccatgacagc
      361 gagacaatgc gccttgaatt tctctgcggg gacctgcttc atggtatttc tcctaatact
      421 acagtagagc aatttcagag tggttgtagc acgggtagtg cgggcctttc agtcccccgt
      481 tgagaccgcc cgcatcgctc ttgggttcag ccgctgaggt cttcgacctc agcggctgaa
      541 cccctttttc tcttcctgtc cttggacgcg ggcctgaagg cccgcactac ccgtacaagc
      601 ggatacccca gtcctggacc actctcgaat tcctctgttg caggctgtag gtgtcagcca
      661 atagaagcca ggttaagccc cgaacatcac ccccaccggc gttcctagtc ctgacgcaag
      721 cttctccgct gccgacgctt gcttcgccgc gatttccaaa tagcccgagc tgccgacgat
      781 ggcgaagaac tcatcctctc caccttccgc gtaggaatgg cacacacgcg tgatcgtctg
      841 acccgcaacc aggatgctca ccgcggatgg cgtagtcgtg aacagcgccg gcgcctcctg
      901 ctcactgatg ttggtgatca ggttgccgaa cttgtccacc ttgagcacca cgccgcgcag
      961 gctgttctcg ccgatgcgct ccacggcggg cagcgtgagg cgcgcatagt cggaaatctc
     1021 cggcccgaac tcggccggcg caacaccctt gctcaaccat cctgcgaccg gcgaaaagac
     1081 gtcccggcca tgaaaggtct ggctcaccgg ctggaggaaa tagcgctcgg cggtgatgtg
     1141 acgcacggtg aattttggct cccgcatctc gaccaacgag agcactccgt tgtccggcgc
     1201 tacgaagacg tagtcgtcgg tctcgacgat gatgggccgg cgtgtgccgc caacgccggg
     1261 atcgaccacc accacgtgca ctgtgcgcgg gggaaagaat cggtacgcct gcgcgatcgt
     1321 ccatgcgcca tcgaacacgt cgtaggaagt tacggcgtgg caaacgtcaa ccagggttgt
     1381 tactggatgg atgttgagca ccacgccatg catgatgccc acgaaaggat cgttcagccc
     1441 gaagtcggtg gtgaaggtga caatgcgcgt gtcagacatg cactcgcaaa gtattcgcag
     1501 cgggcgccgg agtcaagccc gccaacgttg aaaccgccta ccacgtcccc atgcgcagga
     1561 ccaacccggt tgtaacctgc agcccattct gataattcat gccgttcaga tcttgcgtcc
     1621 aggaatgtga atactccagg ctggcgaccc ggaaggccag tgcgttgttg atcttgtagt
     1681 ccactcctgt tcctgccgca atggcaaagc cgttcgtgtc ccaatccttc gtgtaaagat
     1741 ggtgaagagg atatccctgg tcggctggag gggcggcggc gattactgtt gccttttctt
     1801 cagggaacag cctttcctgc gtgagcttgg tgccgccgac caggaactgt gcgtgggcgt
     1861 tccagcggcc agccgtcgag ggcgtccagc ggggtccgat catataactc aacgaatcgc
     1921 cgctgagatt cgtcggaagg tccagcagct tgcaaccgtt aacgtccacc acgatctgcc
     1981 attcggggac aactcggaac gctatcgagc tcccaccgcc tatacacgat cccttggcgc
     2041 cactaccgag ataggttcgg aagttgggag tcatagtaaa ttcaaagggt gctacaccgg
     2101 gcggcgggtt gaccggaacc ctggccgtgt ctcgctccag ggcggccttc gccgcggcgg
     2161 cttcgggata gggcttaaat acgcccggcc ggttgtcacg gtgccaggga accataccat
     2221 tcatcacgtt ggccatcgaa cgcgagggat tcaaccctga acggaccagc aaccgaacat
     2281 acggattgcg gttatgagct tccagagccg ttatgacata acggtccaac gagtcttcgg
     2341 caagcgtcca tcctaggccg actgtcggcg tgatgacatg gtccacaaat ccctgctgtg
     2401 gaaagaacgc ctggacgtgt ccgatggaag cctcgctaat cggaccgatt tcgaactgca
     2461 cactgtaaag gtatgcgaac gcggctcccc ttagctttcc cttccagtat cggctgttct
     2521 tgccgaattc caccgtccgg taggcccgat cattgtgctg ccagatgaat cccgccacag
     2581 cgccttgcat tgggtgaccg acgtagttga ctaagaatgg gtctccgtcg gcccatccgt
     2641 ggaggttgct aaccgaattc agatacccag gccaaaaggg cgtgtcgaaa gcatcacggg
     2701 ttccatattc ggtggcgtac cggaacacgt gctcgacggt caggaatgca aacgatcctt
     2761 ccgccaagtg caaccagtca attccagttt ctcgcttggg actggccggc actgcgacga
     2821 tgcctgttgc ggacgcagta gccgcactgc ccgaatcagc cgggacgttc agggaactgg
     2881 atgaacgttc agccggcagg gacacctcgg acgggtcgcg gggaatcgct agtaccgcct
     2941 gtgcgcgaac ataagcagca ctgctgaagt acaacaaggc caggcaaacg gcaaggacga
     3001 atcgcgctct ggtcaacatc gtcatatcgc acagccaatt aatagatttc agggtcaatc
     3061 gtgtgtcaat agaatgcatc atttgtccac gtttgggaac taggaaaatt atcgtctgat
     3121 cagtcgattg accttagatt tcgcgtgggt ttcggagcaa agaaaagcca cgacgaaagg
     3181 tggctctgcg attaatcctt tagtcttata atc
//
LOCUS       DASRKK010000350         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1004622, whole genome shotgun sequence.
ACCESSION   DASRKK010000350 DASRKK010000000
VERSION     DASRKK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004622"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFC15_07635"
     CDS             <1..312
                     /locus_tag="VFC15_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HZM10066.1"
                     /translation="ENGAGTNPEELIAAAHAGCFSMALSGQLGEAGMRAESIDTKATL
                     TMEKTDAGFTITTVHLDVSVKIPGGDQAKFDQAAQNAKKGCPVSRVLNANITMDAKLI
                     S"
     gene            complement(865..1965)
                     /locus_tag="VFC15_07640"
     CDS             complement(865..1965)
                     /locus_tag="VFC15_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10067.1"
                     /translation="MSETSAHTPTPTPGLLGLFQKRFEGDDALLALAGLRFRQAGLGT
                     ELYARTPEDLEWQWPYRPTPEGLATVHLDRNLNLLEERSHNLIEEFAARCRNRVFGMI
                     VHDQRELQSQPEAYLAAVQRVAAALDKIEGAPRLYIEYAVGLEPAEFAAFFDRTRELE
                     SVSACIDVGHVGIREVRSTYSRKHPGDDVCGLRFDDPLVVPLADEIQEAVRSCLPVVL
                     ELIRTLARHRKPLHFHLHDGHPLSKYSPYSVSDHLSFLAQIPVPFEYDGRCSLPTLYG
                     PEGLFQIVTTALAAIEPGSLSFNLEIHPTEGRLALRDAAHLFQHWQVRSNAERMNYWL
                     SILAENRQLLTAGLRTNSSHGNSGGKLERFQS"
     gene            complement(1962..>4747)
                     /locus_tag="VFC15_07645"
     CDS             complement(1962..>4747)
                     /locus_tag="VFC15_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZM10068.1"
                     /translation="RNFSGSVILEQWPSPPSLLIDARDRLLQLMNDGQPAEAPALAST
                     VVAAETAASPERSSASDGFLPQLAEGNVHARSWREKLELVRSLVAGCGGEITLEELID
                     LSVYLRFLGTGEVACVEDGRHFRPSHAAKASIEIQDALSAVTGPAKTLVLRKIYCWLP
                     STDRAFTRAEPLTRIRDIAHRNDIPSDLKREIKNALQNKLHRCVGPEDLVTSARILDH
                     ITAPGAQYSRDFVEQFQIFHEELKEFFSARSLDEQLRGLAAKVSPENADLIQRFLQAK
                     EQLGEDIPQQVATLRLLTGLRRAFLASVRNNPGSGGHELRLADIALEDLAFPLLSRLL
                     ASLDQLNGELPWPELLEALTLTVSNVGLSGFSAAECDALEAELGAWQQASDPASREHI
                     LRVKATIERSRRLADDYSGWVMGLFPNRVSRLGRALEVAGASIAMFVEGDIRGSLVFQ
                     ISELATRLTRNIRNRLNLSPWEVLVSGSAVGELVAAESLAGLRNPPQGDVIALLRGAE
                     GDEEISGAVRGIVLAHQIPHLSHLGVRARQRRVPFVTCEDTEEFERLEGLRGSVVAME
                     AGAGTFRLTPSAQLPPAQSKTFSAPGAAVRVPEVSLRAQSVCIPLDQATSDTGGGKAS
                     GLRRLAALARRDSAGFLTAPGLVVPFGVMESSLESAPAIRDQYRQLVGQVNGLPPSDF
                     AATAGRLRGLIQQLEVPAGVVQAAVGSFDGASRIMVRSSANCEDLAQMAGAGLYDSIA
                     NVAPGDVADAVRRVWASLWTERAAMSRRENGIAHEQAHMAVVLQEMIAPDYSFIVHTV
                     NPVDQRRDEAYVEIAVGLGEVLASATLPGTPYRMVCDKISGKSRILAFANFSCALRPD
                     AAGGTSMARVRYSEMPLSRDLQVAAALGKRLAALARVIEEEFGSPQDIEGAIVGDQVY
                     LVQSRPQQGLGTA"
BASE COUNT          954 a         1469 c         1481 g          843 t
ORIGIN      
        1 gaaaatggcg ccggtacaaa tcctgaagaa ctgattgcgg cggcgcacgc cggttgtttt
       61 tccatggctc tttctgggca gctgggagaa gccggcatga gggctgaaag catcgacacc
      121 aaagcgaccc tgacgatgga aaaaactgat gccggcttca ccatcacgac cgtgcatctt
      181 gacgtcagcg taaagatccc gggcggcgat caggcgaaat tcgaccaagc cgcacagaat
      241 gccaagaaag ggtgccctgt ctcacgcgtc ctgaacgcca atatcaccat ggacgcgaag
      301 ctcatttctt gatcccctcc catggctgcc cggctcaaac tgggtagcca acattatggt
      361 tcagcctgat ttctcgaggc agcccctgcc aagccggact ctggtggcgt ggatgaaaca
      421 gtttgagttg agcgaccctc tgtcggttca gtgaccttcg catcagtacc ccctggagat
      481 agcaagcaac gatgctcagc ttctagcaca acgtcacttt caggaagcag cgtgccttga
      541 agcagtgact tcagatccag caatgcttta caactgactg tgggcagcta gagcagtttc
      601 agagttagtg tgaactgcgg aagtttcgcc tccgcagcaa taccaacccg gagcaagacc
      661 gacgctcggg tagtgcggcg cttcagcgcc gcggcataag gccccggatg gaaggggcct
      721 ttaggccctg aggttcggtg tttgaatcag ccgaaagcgc ctccgagggc cgccagaccg
      781 aggtacccca ggggctaaag cccggttttt ctaaaacgct attaacgcgg acctgaaggt
      841 ccgcactacc cggccaggtc tacgctaact ctgaaaccgc tctagtttcc cgccggaatt
      901 cccgtgagaa gagttcgtcc gcagccccgc cgtcagcagt tgacgatttt cggccagaat
      961 cgagagccag tagttcatgc gctcggcatt gctgcgaact tgccaatgtt gaaagaggtg
     1021 cgcggcatcg cgcagggcaa gccttccttc cgtgggatga atctccaggt taaacgacag
     1081 cgatcccggc tcgatcgccg ccagagccgt ggtcacgatc tggaagagac cctcggggcc
     1141 atacagcgtc ggcagcgaac agcgtccgtc gtattcgaac ggaacgggaa tctgtgcgag
     1201 aaagctcagg tgatcggaga cgctataagg actgtacttc gagagcgggt gtccgtcatg
     1261 caaatggaag tggaggggct tgcggtgacg ggccaaggtc cgaatgagtt cgagcacgac
     1321 tggcaggcat gagcggactg cctcctgaat ctcatcggca agcgggacaa ccagcgggtc
     1381 gtcgaatcta agaccgcaca cgtcgtcgcc gggatgcttc cgcgagtaag tactgcgtac
     1441 ctcgcgaatg ccgacatgtc cgacatcgat gcaagcgctc acagattcca gttcccgggt
     1501 gcggtcgaag aacgccgcga actcggcggg ttcgagccct acagcgtact cgatatacag
     1561 gcggggcgct ccctcgatct tgtccaacgc cgcggcgact cgctggacgg cggcaagata
     1621 ggcttctggc tgcgactgga gttcgcgctg atcgtgcacg atcattccga agacgcggtt
     1681 gcgacaacgg gcggcgaatt cttcaatcag gttgtgcgat cgttcctcga gcaggttgag
     1741 attgcggtcg aggtgcaccg tcgccagccc ctcaggcgtg ggccggtacg gccattgcca
     1801 ttcgaggtcc tcgggcgtcc tggcgtaaag ctcggttccc aacccggctt gccggaagcg
     1861 cagacccgcc agcgcaagca gcgcatcgtc accctcgaag cgcttctgaa acaggccgag
     1921 gagtcccggc gttggcgtgg gggtatgagc gctggtctcg ctcatgctgt gcccaggccc
     1981 tgctgaggcc tcgattgcac gagatacact tgatcgccca cgattgcccc ttcgatgtcc
     2041 tgcggagagc caaactcttc ctcaatgaca cgcgcgagcg cggccaagcg cttgcccaat
     2101 gccgccgcaa cctgcaggtc gcgagagagc ggcatctcgg agtatcgcac ccgggccatg
     2161 gaagttcctc ccgcggcatc aggacgcaga gcgcaactga aattggcaaa ggccagaatt
     2221 cgactcttgc ctgagatttt atcgcagacc atcctatacg gagttcccgg caacgtggcc
     2281 gatgccagca cttccccgag gccgacggca atctcgacat aggcttcgtc gcgtcgttgg
     2341 tcgacgggat tcacggtatg cacgatgaag gagtagtccg gtgcgatcat ctcctgcagg
     2401 accacggcca tgtgcgcctg ctcgtgggca atgccgtttt ccctgcggct catcgcggcg
     2461 cgctcggtcc agagcgaggc ccagacccgg cggacggcgt cggcgacatc gcccggcgcg
     2521 acattggcga tggagtcata gagcccggct cccgccatct gcgcgaggtc ttcacagttg
     2581 gcgctggaac gcaccatgat ccgcgatgcg ccatcgaagc tccccacggc cgcttgcacg
     2641 actccggcag gcacctcgag ctgctggatc aacccgcgca gccgtcccgc cgtcgccgca
     2701 aagtcggaag gcggcaatcc gttgacttgc ccgacaagct gtcggtattg gtcgcgaatc
     2761 gccggtgcgg attccagtga gctctccatg accccgaagg gaacgaccag ccccggggcg
     2821 gttagaaagc cggccgagtc gcgacgcgcc agagccgcca gacggcgaag tccggaagcc
     2881 ttgccgccgc cggtgtcgga agtggcctga tcgagcggaa tgcaaacgga ctgggcgcgc
     2941 agcgaaactt ccggaactcg tactgccgcg ccgggggcgg agaacgtctt gctctgcgcc
     3001 gggggcaatt gcgcagaggg cgtcaatcgg aacgttcccg caccagcctc catcgcgacg
     3061 acgctaccgc gcagaccttc caggcgctcg aactcttccg tatcttcgca ggtcacgaaa
     3121 ggaacgcgtc gctggcgggc tctcacgcca agatgcgaga gatgcggaat ctggtgcgcg
     3181 agaacgattc cccgcaccgc accggaaatc tcctcatcgc cctcggcgcc cctgaggagc
     3241 gcgattacgt cgccctgcgg tgggttgcgc agaccggcga gcgactcggc tgcaaccagc
     3301 tctccaacag cgcttccgct gaccaggact tcccacggcg atagattcag acgattccga
     3361 atattccgcg tcagtcgcgt ggcaagctcg gaaatctgga agacgaggct gccgcgaatg
     3421 tcaccctcca cgaacatcgc aatggaggcg ccggctacct cgagggcccg tcccaacctg
     3481 gaaacgcgat tcgggaacag ccccatcacc catcccgaat agtcgtcggc cagacgccgg
     3541 ctgcgttcga tcgtggcctt gacgcgcagt atgtgctcgc ggctggctgg atcggacgct
     3601 tgctgccaag ccccgagttc ggcttccagc gcgtcacact ccgctgcaga aaagccgctc
     3661 agccctacgt tgctgaccgt aagcgtcaag gcttctagta gctccggcca cggcaactca
     3721 ccgttcagct ggtcgaggga cgcgagcagg cggctgagca gggggaaagc gaggtcctcc
     3781 agcgcgatat cggcgagacg gagctcgtgg ccgccgctgc cgggattgtt ccgtacgctg
     3841 gccaggaaag cacggcgtaa cccggtcagc agtcgcaagg tggcgacctg ctgcgggatg
     3901 tcctctccca actgctcctt ggcctgaaga aacctctgga tgagatcggc gttctcgggc
     3961 gaaaccttgg cggcgaggcc gcgaagctgc tcgtcgagag agcgggcgct gaagaactcc
     4021 ttcagttctt cgtgaaagat ctggaactgt tcgacgaagt cgcgcgaata ctgcgccccc
     4081 ggcgcggtga tgtgatcgag aatgcgggcc gaggtgacca ggtcttccgg accgacgcaa
     4141 cgatgcagct tgttctgcaa cgcgtttttg atctcgcgct tcaggtcgga ggggatatcg
     4201 ttgcgatgcg ctatgtcgcg aatgcgggtg agcggctccg cccgcgtaaa ggcacgatcg
     4261 gtcgagggca gccagcaata gatcttgcgc aagacgaggg tcttggcggg accggtcacg
     4321 gcggagagcg cgtcctggat ttcgatggac gccttggccg catggctagg gcggaaatgt
     4381 cgcccgtctt ccacgcaagc cacctcgcct gtacccagaa agcgcaagta gaccgagagg
     4441 tcgatcaact cctcaagcgt gatttcgccg ccgcaaccgg cgaccagcga gcgcaccagc
     4501 tccagcttct ctcgccagct ccgagcatgg acattgcctt ccgcgagctg aggaaggaag
     4561 ccatcactcg cgctcgaccg ctcgggcgat gcggcagttt cagcggcgac cacggtcgag
     4621 gcaagcgcag gcgcttcggc aggctggccg tcattcatca actgaagcag ccggtcgcga
     4681 gcgtcgatca gcagcgaggg cggagaaggc cattgttcca ggatgacgct gccggagaag
     4741 ttgcgct
//
LOCUS       DASRKK010000351         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_27093, whole genome shotgun sequence.
ACCESSION   DASRKK010000351 DASRKK010000000
VERSION     DASRKK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27093"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFC15_07650"
     CDS             complement(<1..223)
                     /locus_tag="VFC15_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10069.1"
                     /translation="MRIAHAVCTFLAVVPSNWSADWAWGLPLIVLTVLIHVSGLGLIS
                     QKVVHGNLGRMIQHRHPKIAQVVVLGATTL"
     gene            complement(247..1476)
                     /locus_tag="VFC15_07655"
     CDS             complement(247..1476)
                     /locus_tag="VFC15_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM10070.1"
                     /translation="MMPLAFFVSLLFVYALLSRRAAQTPITAPIFFTAAGMVMSPVWM
                     HVAEAGVTANVFLRLAELGLVMLLFTDASRTDLTVLRRIGTLPARLLSTGLLLTILLG
                     GIVARLVFPGLSIWEAGILAAILAPTDAGLGQIVVSSLRVPMRIRQALNVEAGLNDGL
                     SVPFLLFFMALAAAKIEGGAASLMQFIGEQLGLGVLVGMAVGLIGGWLLRTATRRGWL
                     AESFQQIAVVTLPLLCLVLAEMVEASMFIASFVAGLAIQIPFKEAGKHSVEFAEEWGQ
                     ILNLAVFFMFGMVVVRDWAQFTPASWVYAILSLTVVRMLPVAVALLGTRLSLPSVFFM
                     GWFGPRGLASIVLGLVYLEQELHLPSESAIRATVMLTVLLSIFAHGLSAMPGISIYAR
                     KVAALNSSAPECQPGSQ"
     gene            complement(1473..1622)
                     /locus_tag="VFC15_07660"
     CDS             complement(1473..1622)
                     /locus_tag="VFC15_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10071.1"
                     /translation="MRHRVRYTATTRKVHRRAVHMQKVEAEPEDWCSDGTAKLATVVY
                     IGGLQ"
     gene            complement(2240..3790)
                     /locus_tag="VFC15_07665"
     CDS             complement(2240..3790)
                     /locus_tag="VFC15_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM10072.1"
                     /translation="MPLHIPRPFTSAITVALAIGALVGTRAVAQKSSLSTDQRIQRVE
                     ANLVSIPSPLQFNLQQLMNALNVPGLTVTVIADFKPAWTKTYGVTEAGTSTPVTPQTL
                     FQAGSISKPVAATGALYLVEHGKLSLDGNVNQYLKTWKVPDNEFTTTQKVTLRRLLSH
                     SAGLTVHGFPGYAVGQPIPTTVQIFNGEKPANTDPIRVDFVPGSKVRYSGGGITIEQQ
                     MIVDVSGKPFPEFMHDTVLGPIGMKNSSYQQPLPTSLASLAASGTRADGKTIPGKWHI
                     YPEMAAAGLWTTSGDLATFAINIALSKNGKANNVLSESMTRQMLTAQLDDAGLGFFLN
                     MGGNPNAFGHDGADEGFQAILIMISDTGQGAAIMANSDNGLEIGNYLVNSIAREYGWK
                     YAPPKTDAAGALSLVETTEGAKAAIQEYRYLHENAASQFDFNEGTLNQLGYNLLDKGD
                     TQDAIQLFQMNVQEYPKSWNCYDSLGEAYMKAGQKELAIENYAKSIELNPDNKNGVEM
                     LKKLKEQR"
     gene            complement(4299..4835)
                     /locus_tag="VFC15_07670"
     CDS             complement(4299..4835)
                     /locus_tag="VFC15_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10073.1"
                     /translation="MTDTVALFAGHRVDEPGRTPPRFPPECETKARLAIRSVMRSIAA
                     QGSVLGIAGAADGGDILFHEVCAELGIESQVCLALPSTEYLRTSVQASWVGRFHRLLQ
                     THPVVTLEPGSGDVWLRDHEWQLRWARERHPRRTVLVALLEDIEPEIQGGTSGMVRIA
                     RDSGVEVIRLDARDICGS"
     gene            complement(4832..>5456)
                     /locus_tag="VFC15_07675"
     CDS             complement(4832..>5456)
                     /locus_tag="VFC15_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZM10074.1"
                     /translation="RMVRGEAGAPLPWIVRFGPGGYEEDIVYWTNPVSLSVQVEPRVR
                     AMFRLSRAKRDGKDIVYLTSKRYRDFLVFVSHNLKPPDDLLVRRICDLLRKHNVAAFE
                     YNDRNRAGIEWRPAMDESLRKATHFVALVSPTYDQSPTCTEEIEAALAPGRAVEILPF
                     KVLGRQTPHPRLTGLHNEPLRSEDPEANAEEVVRNVVERLDAALQGG"
BASE COUNT         1130 a         1604 c         1576 g         1146 t
ORIGIN      
        1 acaacgtcgt ggcacccagc accaccacct gtgcaatttt aggatgacgg tgctgaatca
       61 tgcggcctaa attgccgtga acgacttttt ggctaatgag cccgagtcca gaaacatgaa
      121 tgagcacggt cagcacgatc aatggtaagc cccaggccca gtcggccgac cagttcgacg
      181 ggacgaccgc gaggaaggtg caaacggcgt gggcaattct catcgagatc tccttttcac
      241 ggtcggctat tgtgaaccag gctggcactc cggcgcggag gaattgagag cggccacttt
      301 gcgtgcgtag atgctaatgc cgggcatggc actcagcccg tgggcaaaga tgctgagcag
      361 taccgtcagc atcacggtgg ctcgaattgc agattcgctg gggagatgaa gttcctgctc
      421 tagatacacc aatcccagaa caatcgacgc aagcccacgc gggccaaacc atcccataaa
      481 gaaaaccgag ggtagactaa ggcgtgtccc aaggagcgca actgcgactg gcaacatcct
      541 cactaccgtc agactcagga tggcatagac ccaactggca ggcgtgaatt gcgcccaatc
      601 gcgcactacg accatgccga acataaagaa caccgcaaga ttaagaatct gtccccactc
      661 ttccgcgaac tcaacgctgt gtttccctgc ctccttgaac ggtatctgta tggccagacc
      721 cgcgacgaac gaagcaatga acatgctggc ttcaaccatt tccgccagca ccaggcaaag
      781 cagaggcaac gtgacgacgg cgatctgttg aaacgattca gccaaccagc ctcggcgagt
      841 agcggtgcgc agcaaccagc ccccgatcag gccgaccgcc attccgacaa gcactcctaa
      901 acctaactgc tccccaatga actgcattaa gcttgccgcg ccgccttcga tctttgccgc
      961 cgcaagcgcc atgaagaaca gcagaaaggg caccgacagt ccgtcattga gcccggcttc
     1021 aacgttaagt gcctgccgaa tccgcatggg cacgcgcaga ctgctcacga caatttggcc
     1081 tagtccggcg tcggtcgggg caagaatggc ggccagaatg cctgcttccc aaatcgacaa
     1141 acctgggaat accagtcggg caacaatccc gcccagaagt atggtcaaga gcagtccggt
     1201 gctcagcaat cgagcaggca gcgttccgat tctccgcaac actgtgaggt cggttcggct
     1261 ggcgtcggtg aatagcagca tcaccaaacc taactcggcg agccggagaa acacattcgc
     1321 ggtcacgcca gcctcggcaa catgcatcca gactggagac atcaccatcc ctgcagcagt
     1381 gaagaaaata ggggcggtga tcggcgtctg cgctgccctt cttgatagga gggcatacac
     1441 aaagagaagt gagacgaaga aggctagagg catcattgca gtccgcctat gtaaaccact
     1501 gtggcgagct tcgcggttcc gtcggaacac cagtcttcgg gttctgcctc gaccttctgc
     1561 atgtggaccg cccttctgtg cactttgcgg gtggtagccg tgtagcgtac acgatgtctc
     1621 aagattttct caggtgtagt caggcccatc tgtgatgcct gtcacatccc ggcaatgaac
     1681 cgactgcaca acctgccgaa gtctcgtttg attcttaagc aaagcgagga ccgatttggg
     1741 caactcccga gtcgtcgaca actcggagac taggagaatt acttacgttg ttcatttgat
     1801 gaaaaatcgc cttcgcatca gtggggagat gacagctcct caggacgctt ataggggcat
     1861 tccagactta ttacgatagc gagccataac aggccattgc gtaggtaatc cccgctctgg
     1921 gcctgcaaaa gggagtgcag ttgtttggct ggccctcgtt gcacaaagga ccctttgtga
     1981 gacggtgaac cttttcgata atactggggt ttactttccg tgcaaggctc tccagaatcg
     2041 ttaatatcga catactcttg gagggtgtat ttgtcggtgc cggagaagtt gagttctacg
     2101 tgagtggggc cgcacctctc atagggtttg gggattacga tgcttgaaat gtattcagta
     2161 ggagctaacg cgcaccgcac ggtgcattgg ccagccgggt gcccacgtat ttcgccgtcc
     2221 tcgaccgatg ggtatgcggt cacctctgct ccttcagctt cttgagcatc tccactccgt
     2281 tcttgttgtc gggattcagc tcgatcgact tggcgtagtt ctcgatggct agctccttct
     2341 gtccggcttt catatacgct tcgcccaggc tgtcgtagca attccaggac ttggggtatt
     2401 cctgcacatt catttggaag agctggatgg catcctgcgt gtcgcctttg tccagaaggt
     2461 tatacccgag ctggtttagc gtgccctcgt tgaagtcaaa ctgagaagca gcgttctcgt
     2521 gcaggtagcg atattcctgg atcgccgcct tcgcaccttc ggtcgtttcg accagcgaca
     2581 gcgctcccgc cgcgtcagtc ttcggcggag cgtacttcca gccgtactcg cgcgctatgc
     2641 tgttgaccag gtaattgccg atctcgagac cgttatcgga gttggccatg atcgccgctc
     2701 cctggccggt atcgctgatc atgatgagaa tggcctggaa gccctcgtcg gccccatcgt
     2761 gtccgaaggc gttgggattt ccacccatgt tcaggaagaa accaaggccg gcatcgtcga
     2821 gctgcgccgt caacatctgg cgtgtcatcg actcggaaag tacgttgttc gctttcccgt
     2881 tcttcgacaa ggcgatgttg atcgcaaacg tggcgaggtc gcccgaggtg gtccataaac
     2941 ccgctgctgc catctcgggg tagatgtgcc atttgccggg aatggtctta ccatccgcgc
     3001 gcgttccgct ggcggcgagc gacgccagag atgttggcag cggctgttga tacgagctgt
     3061 tcttcatgcc gatgggcccg agcacggtgt cgtgcatgaa ctccggaaac ggctttccgc
     3121 tcacgtcgac gatcatctgc tgctcaatcg tgatgcctcc accggaataa cggactttgc
     3181 tgcccggaac gaagtctacg cggattggat cggtgttggc cggcttctcg ccgttgaaga
     3241 tctgcaccgt tgttggtatg ggctgtccca cggcgtatcc aggaaacccg tgcactgtga
     3301 ggccggcact gtgactgaga aggcggcgca gcgtaacctt ttgcgtggtg gtgaactcgt
     3361 tgtcgggcac cttccacgtc ttcagatatt gattgacgtt accatcgagc gacagtttgc
     3421 catgctccac caggtaaagg gctccggtcg cagccacggg cttgctgatt gagcctgcct
     3481 ggaagagtgt ttgcggcgtg accggtgtcg aggtaccggc ttcggtgaca ccgtaggtct
     3541 ttgtccaggc tggtttgaag tcggcgatga cggtaaccgt cagtcccggg acgttcagcg
     3601 cattcatcag ctgctgcaaa ttgaattgca agggcgaggg aatggacacc aagttggctt
     3661 ccacgcgctg aatgcgctgg tcggtggaca gagagctctt ctgcgcgaca gcgcgcgtgc
     3721 cgacgagcgc accgatagca agcgcaactg ttatagcgga agtgaaaggc cgtggaatgt
     3781 gcaggggcat gacgatcctt cctccgagtc atccagatgg tttacgctgc accgaccatc
     3841 aaagttccgg aagagcgcct catcggtcaa ccccatcgca ggatagctgt tgtgatcagg
     3901 ccattgcgta agtaatcccc gctctgggcc tgcaaaaggg gctgcaattg tttggctggc
     3961 ggtcgttgca caaaggaccc tttgtgagac atcccgaggc tcctccaagc tccgatattc
     4021 cgacatcgcc tggcagttgg ccgtataacg cccgacccgg tgaagtgacg cgaggcaggc
     4081 gatgtcagct tcggggaagt gtggaacatc ctcgcctccc tagtggtaag gcggcgccgg
     4141 ttaatgcaag cgagcggttg ccgcagattc tttatctcgc cgtccatttt agggaatgat
     4201 agcgtccttt atcgttcact acctgtatcg ttcactacct gtgctggtaa gcccgcgagt
     4261 gcaatgtgtg gactatcgca accaatccat tagggagttc atgagccgca aatgtctcgc
     4321 gcgtccagcc ggatcacttc cacgccgctg tcccgcgcga tgcgcaccat ccccgacgtt
     4381 ccgccctgta tctccggctc tatgtcttcc agcagcgcca ccagtaccgt gcgacgcgga
     4441 tgccgttcgc gcgcccaccg cagctgccac tcgtgatccc gcagccagac gtcgccagaa
     4501 ccgggttcga gagtcacgac cgggtgtgtc tggagtaggc ggtgaaagcg cccgacccag
     4561 gaggcttgaa cggaagtgcg gaggtattcg gtcgagggca gggcgaggca gacctgcgat
     4621 tcgataccca gttccgcgca gacctcgtga aacaaaatat caccgccgtc ggccgcgccc
     4681 gcaatgccca gcaccgatcc ctgggcggca atgctccgca tcacggatct gatagccagc
     4741 cgcgctttcg tctcgcattc cggcggaaag cgtggcggag ttcggcccgg ttcgtccacc
     4801 cgatgccccg cgaacagagc cacggtgtcc gtcatccgcc ctgtaacgcg gcgtcgagcc
     4861 gctccacgac atttctgacc acttcctccg cattggcttc cgggtcttcc gagcgcagcg
     4921 gttcgttgtg cagcccggtt agcctgggat gcggagtctg tcggcccaac actttgaacg
     4981 gcaggatctc gactgcgcgg ccgggggcca gcgccgcctc gatctcctcg gtgcacgtcg
     5041 gcgactgatc gtaggtggga ctgaccagtg ccacgaagtg cgtagccttg cgcagcgatt
     5101 cgtccatagc cggacgccac tcgatcccag cacggttgcg gtcgttgtac tcgaaggcgg
     5161 cgacattatg cttccgcagc aggtcacaga tgcggcgcac cagcaggtca tccggcggct
     5221 tcaggttgtg gctcacgaag accaggaaat cgcgataacg cttggaggtg agatagacga
     5281 tatccttgcc gtcccgcttg gcgcgactca aacggaacat ggctcggacg cgcggttcca
     5341 cctgcacgga caggctgacg ggattagtcc agtagactat gtcctcctcg taaccgcccg
     5401 gaccaaagcg gacgatccat ggcagcgggg cgcccgcttc gccgcgcacc atccgg
//
LOCUS       DASRKK010000352         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_612750, whole genome shotgun sequence.
ACCESSION   DASRKK010000352 DASRKK010000000
VERSION     DASRKK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612750"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFC15_07680"
     CDS             <1..242
                     /locus_tag="VFC15_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10075.1"
                     /translation="QVTCGPGDQQPFSLEARKDDNCCKQPISHLIAKLGPQRGLRVRW
                     RFHNTYLCRPQIAILNGMHRHKAGGAFAPLQPITI"
     gene            645..2120
                     /locus_tag="VFC15_07685"
     CDS             645..2120
                     /locus_tag="VFC15_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZM10076.1"
                     /translation="MTPSVVAMTIIFGIILVGAITGVYAGFRRQMSLEQWTVGGRGFG
                     LVLIWLLMAGEVYTTFAFLGASGWAYSRGGPTLYIVAYLTLAYVVSFFILPRIWEAGR
                     KFSLHTQADFFQQRYGSKYLAALVAVVGFLFIIPYLQLQLTGLGLIVQIASFDAISRP
                     LAITIAFIVVAGFVLSSGIRAVAWVSVLKDTLMLVAAFSIGIGVPYHYFGGIKPMFQA
                     LVQAKVQHLTMPGSTATMGHTWYISTVLLTSMGFYMWPHAFCSVFTAKSGNTLRRNAV
                     IMPLYTITLPLLFFVGYTAILVTPGLKNGDLALLSTVRKTFSPWFLGLVGGAGALTAM
                     VPAAILILAAGTLFAKNFFRPLFAPSMSDAGVAKLAKVMVVVITALAMYFAIYSSTTL
                     VGLLLFAYSGIAQFFPGVVLGLFWKRVTALGVFAGLVVGVGCDMFLIFTKHDPFHGLN
                     AGFIALCLNLIVTTVVSATTAAEPDRFEEQVETAVAAEAVM"
     gene            3718..4104
                     /locus_tag="VFC15_07690"
     CDS             3718..4104
                     /locus_tag="VFC15_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10077.1"
                     /translation="MLSDPAFSGHASELVEALVKAGGTGVGPDLDNLLDQELAFWKST
                     APSLRQGWWNVDANIHAPLREQYGKTLNLVQGIAEIHYSPAMPRVIELRNFWRSQPQL
                     NDPSGTDQMAEEAEECDKSIKQLQAN"
     gene            4295..5248
                     /locus_tag="VFC15_07695"
     CDS             4295..5248
                     /locus_tag="VFC15_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZM10078.1"
                     /translation="MAKLQQREDQVFLVLALVIGALTGLTVVAFILLTEREGLRLYPA
                     GGAPWRRVLFPVAGSLGIGYLLFRYFPNARGSGVPQTKAALFAREGRITLRTVLGKFF
                     CTSATLASGIPLGREGPSVQVGAGIASVLGRRLGLRPEKVKALLPVGAAAAIAAAFNT
                     PLAAVLFSLEEIMGDLHAPVLGSVVLASATSWLVLRLLLGNNPLFKVPQYQLIGPLKQ
                     QVEEWQAMQLSTASAKLLIYQAFIEDELGFPKHLARRTFSPCMRSSRREPCGASRMLS
                     PVLSRSWIPFHSTRQRPSWRAFCNRSALHSAQRKDRRTVLE"
     gene            complement(5415..5651)
                     /locus_tag="VFC15_07700"
     CDS             complement(5415..5651)
                     /locus_tag="VFC15_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10079.1"
                     /translation="MPYAEIGIRYDLVVIRKGLLADSAFSVLLNNFPVQQFPHLCRRP
                     KFAISSWVVWIFDALDPKPKSAFFPNVLPTAAEE"
     gene            complement(5735..6724)
                     /locus_tag="VFC15_07705"
     CDS             complement(5735..6724)
                     /locus_tag="VFC15_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10080.1"
                     /translation="MSALAPTLQAFFTDRLIRQRQASNHTLASYRDTLRLLVRFAAER
                     RKVEPVKLEISDLDSALIGAFLDHRERECGNSVRTRNTRLTAIRSLFRYAALRHPEHA
                     EVIQHVLAIPPKRFARRLVSFLTDPEVDALLSAPDRTTWVGRRDHTLFATAVQTGLRA
                     SELIGLRITDVHLGVGPHVSCLGKGRKQRITPLTTSVVALLRTWLCERREQVSDLVFT
                     TRTGRMLSRDALEHRLAECVAKASNNCPSLREKRISLHVLRHTAAMRLLHAGNDLSVI
                     ALWLGHEQMETTQIYLHVDLALKEKALARTTPVNTSPGRYRPPDTLLAFLEAL"
     gene            complement(6721..7647)
                     /locus_tag="VFC15_07710"
     CDS             complement(6721..7647)
                     /locus_tag="VFC15_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10081.1"
                     /translation="MSPLRQALFDYLAVRRSLGFKLGRAAGLLAQFITYAEERGEQHL
                     RISTMLAWATLPVNRHRNWLAKRLSIVRGFASHLHTIDPATEVPPADLLPWQRCRATP
                     YLYFDDEVAALVTAATRLQTPYRVLTYQTLVGLLAVTGMRVGEALALDRDDIDMTHGV
                     LTVRLTKFGKSRELPVHPSTIQALGRYLCHHDRPRAAANVPAVFVSNTGTRLSYPNVQ
                     WTFQRLLRRAGITRRSASCRPRIHDLRHRFAIQTMLDAYRSGKDAEARLAILSTYLGH
                     VNPKATYWYLSAAPELLQLAADRLQRHLGGVS"
     gene            complement(7644..>8342)
                     /locus_tag="VFC15_07715"
     CDS             complement(7644..>8342)
                     /locus_tag="VFC15_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10082.1"
                     /translation="QSRSVASTTVTALRSFLGYLYANGLIKQQLTAAVPAVARWRLAA
                     LPKALQPREVQALLAACDRRTRMGRRDFAVLTMLVRLGLRASELAGLRLDDIDWRAGS
                     ILIRGKGNQVEPLPCPQDVGDAVVAYLRRGRPASAADRSVFVRIRAPHRALSGTGVSG
                     IVVAAARRAGLGEIRAHRLRHTVATQLLRAGSSLPEIGQLLRHRHISTTAVYAKVDRD
                     ALRSIARPWLGGAA"
BASE COUNT         1699 a         2323 c         2475 g         1845 t
ORIGIN      
        1 atcaggttac ctgtggaccg ggtgaccaac aaccgttttc tttagaagca cgtaaggatg
       61 ataactgttg taaacagccc atctcgcacc taatcgcgaa gctggggcct cagcgcggat
      121 tgcgtgtgag atggcgattt cacaacacct atctgtgccg accgcagatc gcaatattga
      181 atggaatgca ccgccacaaa gcgggcggcg ctttcgcgcc attgcagccc atcacgattt
      241 agcctcttgt gctgtggact gctcattggt tatggttcac tatcccgtgt cacttccgta
      301 gctctaaagc agcttgctca gtcgttgagt tgtgagccgc aatgggaagc agaacctgtg
      361 cgcggcgctg tctttggttg gttgccaagg aacatttagt caagaggaat aatgagtcga
      421 aacggtgttt tgtctctgct attggcattc gtgcctttcg tggccatgtg cttttcggtg
      481 gcgctttggg accgtgtcta tcccctagtt ttcggtctgc ctttcaacct cttctggctc
      541 gtcgcttgga tcccgcttac cagcctaagc atgtggggtg tctatcacct ttggaatcgc
      601 catgaagatt cccgcacgtc cccggccggc aaggagcccc cgaaatgacg cccagtgtag
      661 tcgccatgac gatcatcttc ggaatcattc tggtgggagc gatcaccggc gtctacgccg
      721 gattccggcg gcagatgagc ctggagcaat ggacagtggg cgggcggggc ttcggtctcg
      781 tcctgatctg gctgctgatg gcaggcgagg tctatacgac gttcgcgttt ctcggcgcca
      841 gcgggtgggc ctattcgcgg ggagggccga cactttacat tgttgcatac cttacgctgg
      901 cctacgttgt ctcgttcttt attctgccca gaatctggga ggccggacgc aaattctcgc
      961 tccacaccca ggccgacttc ttccagcaac gctacgggag caagtatctg gcagccttgg
     1021 ttgcggtcgt cggcttcctc ttcattattc cgtacctgca actgcagctt accggtctgg
     1081 gcctcatcgt ccagatcgcc agcttcgacg ccataagtcg tccgctggcc atcacgatcg
     1141 catttatcgt cgtggcagga ttcgtactgt ccagcggcat tcgcgctgta gcgtgggtca
     1201 gcgtgctgaa agacaccctg atgctggtag cggcattcag catcggcatc ggagttccct
     1261 accattattt cggcggaatc aagccgatgt tccaagctct ggtgcaggcc aaggtacaac
     1321 atctgacgat gccgggaagt acggcgacca tgggacacac ctggtatatc tccacggttc
     1381 tgcttacctc catgggcttc tatatgtggc cgcacgcctt ttgctcggtg tttacggcga
     1441 agagtggcaa tacgctgcgg cgcaacgccg ttattatgcc gctctacacc attacgcttc
     1501 cgctactatt cttcgtcggc tatacggcta ttctcgtcac gccgggactc aagaacggcg
     1561 acctggcact gctctcaacg gtccggaaga cgttctcgcc ctggttcctg ggcctggtgg
     1621 gcggagccgg tgcgctgacc gcgatggtgc ctgccgccat cctgatcctc gctgcgggca
     1681 cattgttcgc aaagaacttc ttccgtcctc tcttcgctcc gtcgatgagc gatgcgggcg
     1741 tagctaagct ggctaaagtc atggttgtgg tgataacggc gctggccatg tattttgcca
     1801 tctatagctc cacgacgctg gttggcctgc tgctgttcgc ttacagcgga attgcgcagt
     1861 tctttcccgg cgtggtgctt ggtctgttct ggaaacgcgt gactgctctg ggggtgttcg
     1921 ctggcctggt ggtaggagtg ggctgcgaca tgttcctgat attcaccaag cacgatccct
     1981 tccacggatt gaacgccgga ttcatcgcac tctgcttaaa cctcatcgtc acaaccgtcg
     2041 ttagcgccac gactgctgca gagccagacc gattcgaaga acaggtcgag acagcggtcg
     2101 ctgctgaagc cgtcatgtag gaatctttgc gacaagagtc tgctcagaac gaggctccag
     2161 agctgtgttc ggatgacggt caatcctaag gttggacatt tcaggaatag ctgggggtaa
     2221 caccccaagt cgtaaccaat cccgaacgag acgctcatcc aggattactt acgacacggc
     2281 gatttcacga caacgatgca ggggcgcaca atggcggagt acgcgaccat ttgtgggccg
     2341 ttgcacccta taatcaatcg caatgcaaga accagcaaaa gggcgtgatc tcgggtggcg
     2401 ttcagtttgg aagatgttcc ttccgtggac agtgtccgtg tatgcgctat cagccctcac
     2461 gattgatttg gtgcttccat cgcagggctt gttgggagaa cgggggtttc acctaacatt
     2521 ccttatcccc gcagtatgcg tggggagcat cggcaccgtg gtgttaactg atcgggcacc
     2581 gcagacgagg ttgggcgggg cactcctggg agctgtcacg ggcatgattt cgtcgttgct
     2641 gggtggatat gtggcagaca cagtcctcac ccagccacac tctgagggga ggagttggtt
     2701 tggccttctc atgttccctg tagtcctttt ttcaaccctc attggcgggt tcatgggcgt
     2761 ctatgcccac cggctcggca tcggcagtac ttcttccgac agagctagct ctcaatcctg
     2821 aagcatcacg gccgaaaagc gacgaagtgg taggcggtac cggtcgagga cgttccggac
     2881 gttcagcggg tagcgagtga catggcgatt tcacataaac tgaccagacc cgcaccgcat
     2941 tgtggcgatt cccaccgact ggaatccacc gtgacgaatt gcggtcgttt tggtagggct
     3001 atgcgcacat ccccttggtg cctaatactg gcgctgttgt tggttctgcc ttatccagca
     3061 ggagctgcta ttcgcccgtc gttcgtgctc gactattcat ccttccatgc aactgatatt
     3121 gtgctggtcc gaaccgtgac cgatggggcc tcgtttgaag ttcttgaatc atggaagggc
     3181 gcttgtggtg tgggcgaaag catcgtgctc ccggagcttc gaccgagtgc aaacgctctt
     3241 cccattactg tctatcccca atcgtggcca aacggcaatg ggtgggaaac catccccaag
     3301 cagcctcctg gctcacgcat gattctgttc ctgcgtaaga gtgcgtcgca acccaatccc
     3361 cgacacagtt ctcaatcaga tgggcgtatt gggtggcagc cttcgaattt aatgaattcg
     3421 tggaaggctt ctgttgtgtg ggtcgacggg cttcagatgt atggcttcag acagaacatc
     3481 aacccgggac cgagcaccct cgacaccttg ccatattcgt tggagcaatt gcggaagcga
     3541 gtgtcagagg tcgtcggtat tcagcgagac atggcgacgg cgctggtcgt tcgggacggc
     3601 caccagaggg ctgaggctct caagccatat gttcgttcgg agatctttcc tgtctagcag
     3661 tttgccctag accatttgag tagttgtggg gcctctgcgg taccgacgat tcgaggaatg
     3721 ctatccgatc cggccttttc cggtcatgcc tcggaattgg ttgaagcgct cgtgaaggca
     3781 ggtggcacgg gagtcggacc agatctcgat aacctcctgg atcaagaatt ggctttttgg
     3841 aagtccactg ccccttcgct tcgccaaggt tggtggaacg ttgatgcgaa catccacgcg
     3901 ccacttcgcg aacagtacgg caagacctta aatcttgtac aaggcatcgc ggaaatacac
     3961 tactcacctg cgatgccaag agttattgaa ctgcgaaatt tttggcgttc tcaaccacaa
     4021 ctcaatgacc catccggtac agatcaaatg gccgaggagg ccgaggaatg tgacaaatcg
     4081 ataaagcagt tgcaggccaa ctagataacg agtgtgaaca ggccatagcg tacgttaatc
     4141 ggacttctac gggtgcgcat cgcgcctgct accctcgctt ccgccttcca aaggcagtcc
     4201 actgaggtat gctcttctgg gacatttctc agagatgagt gatagcggcc agacgacgag
     4261 cggaagttgg tggagaaccc cgcggtcgta cttcatggcc aagttgcaac agcgtgagga
     4321 ccaggtattt ctggttctgg cgctggtgat cggcgcgttg accggcctga cggttgtcgc
     4381 tttcattctg ctgacggaac gagaagggct gcggctgtat cccgcaggcg gcgctccgtg
     4441 gcggcgtgtg ctgttcccgg tcgcaggctc gctgggcatc ggctatctgc tgttccgcta
     4501 ttttcccaac gcgcgcggca gcggagtgcc gcaaaccaag gctgcgctgt ttgcccgcga
     4561 aggccggata acgctgcgca ccgttctggg aaagttcttt tgcacatcgg caacgctggc
     4621 cagcggaatc ccgctggggc gcgaagggcc ttcggtgcaa gtgggcgcgg gcatcgcttc
     4681 cgtgctggga cgccgcttgg gattgcggcc tgaaaaagtg aaggccctgc tgccggtcgg
     4741 tgcggcggca gccattgcgg cagcgtttaa tacgcctctg gcagcagtat tgttttcgct
     4801 ggaggagatt atgggcgacc tccacgcgcc ggtgctggga tcggtggtgc tggcctccgc
     4861 tacctcctgg ttggtactcc gccttctgct cggcaacaac cctctgttca aagtgccgca
     4921 ataccaactg atcgggccgc tgaagcagca agtcgaggaa tggcaggcga tgcagttgtc
     4981 gacggcatca gcaaagctgc tgatctacca ggcgttcatc gaggacgaac tggggtttcc
     5041 gaagcacctg gcgcggcgta ctttcagccc gtgcatgagg agttccaggc gcgaaccatg
     5101 tggagcctct cgaatgcttt caccagtgct ttcaaggagc tggatcccat tccacagtac
     5161 aaggcaacgg ccaagctggc gggctttctg caatcggtcc gcccttcata gtgcccagcg
     5221 gaaagatcgg cgtacggtcc tggagtagtg ccgacgatcg cagccaattg aagaagctgc
     5281 ggtggcggaa ggaccgtacg tcgcgggcgc ttctaacagt gccacatttc cagccaagga
     5341 gccggggtca atccaaatca cactggagca tatcaatgaa actccgtcgg aatgaatact
     5401 gtccactgca cagatcactc ttctgctgcg gtcgggagca cgttcggaaa gaacgcagat
     5461 ttcggcttgg ggtccagcgc atcgaagatc cacaccaccc aagaggatat cgcgaacttc
     5521 ggtcgccggc agagatgcgg aaactgctga accggaaaat tgttgagcaa gacggaaaat
     5581 gcgctatctg ccaacaagcc tttacggatt acaacgagat cgtaccttat gccgatttcg
     5641 gcataaggca cggaatgccg actttcagaa tatgccgatc cggcaggcac acaggcgcta
     5701 aacgggggtc cgcggcagaa cgatgtcggc atattcagag cgcctcaaga aacgcgagga
     5761 gcgtatcggg tggccggtaa cggccgggac tggtatttac gggtgtggtc ctcgcgagtg
     5821 ccttttcttt gagagcgagg tcgacatgaa ggtagatctg cgtagtttcc atctgctcgt
     5881 ggccgagcca gagagcgatg accgagagat cgttcccggc atgtaagagt ctcattgctg
     5941 ccgtgtgccg cagcacatga aggctgatcc tcttttctcg taaggacgga cagttattac
     6001 tggcctttgc aacgcattct gcaagtcgat gttcgagtgc gtcgcggctg agcatccttc
     6061 cagtacgcgt agtaaaaacc aaatcgctaa cttgttcacg gcgttcacag agccaggtcc
     6121 ggagcagcgc caccactgac gtggtgagcg gcgtgatccg ttgctttcgt ccttttccga
     6181 ggcaactgac gtgcggacct acgcctagat gaacgtcagt gatcctcaac ccaatcagtt
     6241 ccgatgcacg aagtcctgtc tggactgccg tcgcgaacag cgtgtggtcg cgtcggccaa
     6301 cccaggtcgt tcggtctggt gccgaaagaa gtgcgtcgac ttccggatct gtgagaaagc
     6361 tcacaaggcg acgcgcgaaa cgcttcggtg gaatcgcaag tacgtgctgg atgacttccg
     6421 catgctccgg atgacgaagg gctgcgtagc ggaacagtga ccgaatcgcc gtaagccggg
     6481 tgttgcgtgt gcgtacgcta tttccacact cgcgttcacg atgatcaagg aaggcgccga
     6541 tcagtgcgga atccagatcg ctgatttcca gtttcaccgg ttctactttt cggcgttcgg
     6601 ctgcgaaacg cacgagcaat ctcaaggtgt cgcggtacga cgctaacgtg tgattgcttg
     6661 cttgccgctg gcgaatgagc cggtcggtaa aaaacgcctg caacgtcggg gcgagcgcac
     6721 tcatgagaca cctccgaggt ggcgctggag gcgatcagcg gctagttgta atagttcggg
     6781 tgcagcagac aaataccaat aagtggcctt cgggttcacg tggccaagat aagtcgataa
     6841 gatcgcgagc cgcgcttctg catctttgcc ggaccgataa gcgtcaagca tggtttggat
     6901 tgcgaatcga tggcgaagat catggattct ggggcggcac gacgccgatc gccgcgtgat
     6961 tccggcacgg cgtaggagcc gttggaaagt ccattgaaca ttggggtacg acaatcgagt
     7021 tccggtattc gaaacaaaca ccgcagggac atttgcggcc gcacgtgggc ggtcgtgatg
     7081 gcaaagatag cgacctagcg cctggatagt gctcggatga accggcagtt cgcgcgactt
     7141 tccgaacttg gtcagccgaa cggtcagtac accgtgggtc atatctatgt cgtcacggtc
     7201 gagcgcaagg gcctcgccga cgcgcatccc ggtgaccgcc agtaatccga ccaaggtctg
     7261 atacgtcagc acccgatagg gcgtttgtaa tctcgtcgct gcagtaacca gcgcggccac
     7321 ctcgtcgtcg aagtagaggt aaggcgtagc gcgacatcgt tgccagggca acagatcggc
     7381 tggcggcact tcggtcgcag gatcgatcgt gtgcaggtga gaagcaaagc cacgcacgat
     7441 tgaaagcctc ttcgccaacc agtttcgatg cctgttcacc ggcagggtgg cccaagcaag
     7501 catggtggag atgcgcagat gttgctcccc cctctcctcg gcataagtga taaattgcgc
     7561 gagcagtccc gcggccctgc ccaacttgaa cccgagggaa cggcggacag cgaggtaatc
     7621 gaagagagct tgtcgtagcg ggctcatgct gcacctccta gccacgggcg cgcgatgctc
     7681 cgtagtgcgt cgcgatcgac cttggcatag accgctgtag ttgaaatatg gcggtggcgc
     7741 agcagctggc caatctcagg caatgaggac ccggcacgaa ggagctgggt agccaccgtg
     7801 tggcgcagtc gatgtgcacg gatctcgccg aggcctgcgc gacgggccgc cgcgacaaca
     7861 atcccgctca ccccggtgcc gctaagagcg cggtgaggtg ctctgatgcg gacgaataca
     7921 ctacgatccg ccgcgcttgc gggccgtcct cggcgcagat aggcgaccac tgcgtcgccg
     7981 acatcttgcg gacacggtaa cggctcaacc tgattgccct tccctcgaat aaggatggag
     8041 ccagcacgcc aatcgatatc atcgaggcga agtcccgcga gttcactcgc acgcagtccg
     8101 aggcgaacga gcatcgtcag gacggcaaaa tcccgacgtc ccattcgcgt gcgccgatca
     8161 cacgctgcca aaagcgcctg aacctcgcga ggttgcaggg ccttcgggag cgcagccagg
     8221 cgccatcgag ctaccgcagg aacagccgct gtcaactgct gtttgatcaa gccgttggcg
     8281 taaaggtagc caaggaatga ccgcaatgca gtcaccgtcg tgcttgcgac gcttcgcgat
     8341 tg
//
LOCUS       DASRKK010000353         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_418284, whole genome shotgun sequence.
ACCESSION   DASRKK010000353 DASRKK010000000
VERSION     DASRKK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418284"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFC15_07720"
     CDS             complement(<1..467)
                     /locus_tag="VFC15_07720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM10083.1"
                     /translation="METAALGMIGQIVSHYRIVGKLGGGGMGVVYDAVDTRLGRHVAL
                     KFIPDSLLNDPKSLDRFVLEARAASQLNHPGICTIHDIEHLDGHPFIVMEKLEGTSLK
                     DRMKGGPMEVDEILDIGIQVADALAACHAKGIIHRDIKPANIFLTESGQAKIL"
     gene            907..1200
                     /locus_tag="VFC15_07725"
     CDS             907..1200
                     /locus_tag="VFC15_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZM10084.1"
                     /translation="MMTLDWTRGELRKGLDCFDSGAFFEAHEHWELVWLVAGEPEKTF
                     LQALIQVAASFYHSQRGNQVGTVSLLRSALRRLERYPENFGGIAIAPLRVSIQ"
     gene            1166..>2238
                     /locus_tag="VFC15_07730"
     CDS             1166..>2238
                     /locus_tag="VFC15_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZM10085.1"
                     /translation="MPSRRFVFPSSKHIDKKELIAVSREAVQTAEDAREIAVKKLITE
                     HHSNELQASAEAVDNATRQKELAQAETAKAKSDMERSQANAANQVAAAQADTEQARLA
                     ETQAQRQLQVADNDKAAMRERLSLQLNTILQTRDSARGLIVNMSDVLFDTGKYSLKPG
                     AREKLSKVAGILIAYPGLNIAVGGYTDNVGTDRAIFIDGEAGPRLSRIRACFAASGGA
                     PIRKASSMPVGNDERHRTKRIGWLRAAVLGANDGILSTSSLVLGVAAAHANHRSVLVA
                     GVAGLVAGSMSMAAGEYVSVHSQADTEQADLKLERAELKADDKGEHKELQAIYVERGL
                     DRSLAKQVAEQLMAHDALGAHAR"
BASE COUNT          495 a          641 c          654 g          448 t
ORIGIN      
        1 tcgagaatct tggcctgtcc gctctccgtc agaaagatgt tggcaggctt gatgtcgcga
       61 tggatgatcc ctttggcgtg gcaagcggcc aacgcatccg ccacttggat gccgatgtcg
      121 aggatctcat ccacctccat cggccccccc ttcatccggt ctttcaggct ggttccttcc
      181 agcttctcca tgacgatgaa gggatgcccg tccaaatgct cgatgtcatg gatggtgcaa
      241 atgccgggat ggttcaactg cgaagcggcg cgagcttcca gtacaaagcg atcgagggac
      301 ttagggtcgt tgagcaggct gtcgggaatg aacttcaagg caacatggcg gcccaggcgc
      361 gtatcgaccg cgtcatagac cacgcccatg ccaccaccac caagcttccc cacgatacga
      421 tagtgagaaa ctatctggcc gatcatcccc agggccgcgg tttccatttg gcggttatgt
      481 taggctgcgt acgaaatccg gtcaactcgc ggcacccgcg gcatccgtcc cggccgccag
      541 cctccacttg gtgcatggtt tccacagatt tggtgccggg ggggacttga accccacaac
      601 cctttcgggt cggcggattt taagaccgaa ccacacaaat caaaagatac tctaaatgaa
      661 tgagttacag gcacttagct aaaagtgaca gtttggtctt gcagtgcaaa atgcggctgt
      721 tggacggtct gtacgtcacg tttacgtcac gccgcgctcg tcatctacct ccatgcggaa
      781 gcgcgagggc accgacgatt ataggaactc cctcggccta tcctatccct cgtgtacaag
      841 agggtctggc tacaattaac tcgtgcatga ttatcttcca gcagacgaga ttgcgatatc
      901 cgcctcatga tgacactgga ttggacgcgg ggcgagttac ggaagggtct ggactgcttt
      961 gattccggtg ctttcttcga ggcacacgaa cactgggaat tggtttggct cgtggcaggc
     1021 gagcctgaaa agacgtttct gcaggcgctg attcaagtgg ccgcctcctt ctaccactct
     1081 cagcgaggca accaggtggg tacggtttcg ctgctgcgat cggctttgcg ccgtttggag
     1141 cgatatccgg agaactttgg cggcattgcc atcgcgccgc ttcgtgtttc catccagtaa
     1201 gcacatcgac aaaaaagagc tgatcgccgt ttcgcgcgaa gccgtacaga cggcggaaga
     1261 cgcccgcgag attgcagtca agaaattgat cacggaacac cactcgaacg aactgcaggc
     1321 gtcggccgaa gctgtagaca acgcgacccg gcagaaggaa ctagcacagg cggagactgc
     1381 caaggcgaag tcggacatgg agcgcagcca agccaatgca gccaatcaag tggcggcggc
     1441 gcaggcagat actgagcagg cgcggcttgc ggaaacgcag gcccagcggc aactgcaagt
     1501 tgccgacaat gacaaggcag ccatgcgcga gcggctgtca ctgcaattga acacaatcct
     1561 gcaaacgcgc gactcggcac gtgggttgat tgttaatatg tctgatgtct tgttcgacac
     1621 cggcaagtac agcttgaagc cgggtgcacg tgagaaactc tccaaggtcg ccggaatcct
     1681 gatcgcttat cctggactca acattgccgt aggtggatac accgacaatg tgggcacgga
     1741 tcgcgctatc tttatagatg gcgaagccgg tccacgtctt tcccgaatcc gagcgtgttt
     1801 cgcagcttcc ggcggcgccc cgatcaggaa ggcctcaagc atgcccgtag gcaatgacga
     1861 acgacaccgc acaaaacgta ttggctggct gcgcgcggcg gtactgggcg cgaacgacgg
     1921 catcctttcc acctcgagtc tggtgctcgg tgtcgcagcc gcgcatgcga atcaccgcag
     1981 cgtattggtc gccggagtcg ccggcctggt agccgggtcc atgtcgatgg ctgccggtga
     2041 gtacgtgtct gttcactcac aggccgacac cgaacaggcc gacctcaagc ttgagcgcgc
     2101 ggaactcaag gcagacgaca agggcgaaca taaggaattg caagcaatct atgtcgaacg
     2161 cgggctcgat cgttcgctcg cgaaacaggt tgccgaacag ttgatggccc atgacgcgtt
     2221 aggcgcgcat gcccgcga
//
LOCUS       DASRKK010000354         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_222059, whole genome shotgun sequence.
ACCESSION   DASRKK010000354 DASRKK010000000
VERSION     DASRKK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222059"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFC15_07735"
     CDS             <1..214
                     /locus_tag="VFC15_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10086.1"
                     /translation="FGVITGLRHMNSREIHLVEDGSEHREDAEVMLARADEVIESERD
                     LLQCMSLFLARTGPPVMSAVRSRSGE"
     gene            624..1796
                     /locus_tag="VFC15_07740"
     CDS             624..1796
                     /locus_tag="VFC15_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HZM10087.1"
                     /translation="MRATPICLVLMAAALSLGSPVGRLDARAAEARVCGPVGAWVIPG
                     SRKKQDGVLKSLAKHDVVLLGESHSEAEHHRWQLHTIAALFNYRPDMVLGFEMFPRSV
                     QSVLDRWTKGELDETAFLREVDWQQIWGFDPELYMPLFHFARMHRLPMLALNVDRETN
                     RRVAQRGPAALDSEREGVGVPAAASSSYRGRLFELFKTHPAGGTQANADSEQFSHFVQ
                     AQLFWDRAMAEAIAGALRKGQRPLVVGIMGSGHVEYGDGVAHQLAALKVNDVVTALPW
                     NSGTDCPPAVLRIANLIFGLAPSAATRTPPPRLGVVVSKAETGVKIDEVVTQSVAETT
                     SLQAGDVIETAAGVSVRRPADLIAIVQRQAPGTWLPLTVRRDEARLEMVARFPAER"
     gene            1793..>2153
                     /locus_tag="VFC15_07745"
     CDS             1793..>2153
                     /locus_tag="VFC15_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10088.1"
                     /translation="MMLLQRLLLAIGLASSLAPASAAGEPHYIVQLSLQPALRELHVR
                     MRVSLPAGAERRFQLGRGFAVKTMAIDGQTVDPVRQTWPLPTGRSVEIVYTATLPGQG
                     AVQAIGELAPFADPEGSY"
BASE COUNT          408 a          614 c          700 g          431 t
ORIGIN      
        1 ctttggggtc ataaccggac ttcggcatat gaactcccgg gaaatacacc ttgtcgaaga
       61 cggctccgag caccgtgagg acgccgaggt tatgctcgca cgcgcagatg aggtgatcga
      121 atcggaacgt gatttgctgc agtgcatgag tctcttcctg gcacgaaccg gaccaccagt
      181 gatgtccgca gttcggtcgc gatcagggga atagcggaca tggcacggac ccgccaattt
      241 ggttgagaat gacccaaatg cgacatcgtt gcactgcacc gcccccacac gggatgatcc
      301 ccctggacgg cctgagaaga tgaacattgt gttgcttgac tggttgcagt ccgtgcgtgc
      361 gcggaatgcc gatatcatcg tgcgatcgcg accgtcggat cccaccgcga gatcggatca
      421 gcgcattccg gatagggcag cgcgcaattc aagcaacgcg gcaatggcag tgcagcgatg
      481 gcacccctaa acaattcacc cgcaaccacc gtcatcaagt gacgtcgaat gacaaaagca
      541 acaaaacgag tgatttcagc cctggttttc gctcatactc aatcaaacca gtcctttcag
      601 tcgcgcggca agggatgaga tcgatgcgag ccaccccgat ttgcttggtc ctgatggcgg
      661 cggccctgtc gttgggaagc cctgtcggac gacttgatgc ccgcgcggcc gaggcccgcg
      721 tttgcgggcc ggtcggtgcc tgggtcattc ccggaagcag gaaaaagcag gatggcgtgt
      781 tgaagtcgct ggcaaagcac gatgtcgtgc tgctcggcga gtcccacagt gaggcggagc
      841 accatcgctg gcagcttcac acgatcgcgg cgctgtttaa ttaccggcct gatatggtgc
      901 tgggtttcga gatgttcccg cgaagcgttc agtcggtgct tgatcgttgg acgaaaggcg
      961 aactggatga gaccgccttt ctacgcgagg tcgattggca gcagatttgg ggtttcgacc
     1021 cagaactcta tatgccgctg tttcattttg cccggatgca tcgtttgccg atgttggcgc
     1081 tcaatgtcga tcgggagacc aaccggcgag ttgcgcaacg aggaccagcc gctttagaca
     1141 gcgagcgcga gggcgttgga gttccggcgg ccgcctcgtc gtcatatcgc ggtcggctgt
     1201 tcgagttgtt caagacgcat ccggctgggg gcacgcaggc gaatgctgac tcggaacagt
     1261 tctcgcattt tgtccaggcg caattatttt gggatcgcgc tatggctgag gcaatcgccg
     1321 gcgcactgcg caaagggcag cggccgctgg tcgtcgggat catggggagt ggccatgtcg
     1381 agtacggcga cggcgtcgcc caccaactcg ctgccctcaa ggtgaatgat gtcgttacag
     1441 cgctgccctg gaattcgggt acggattgtc cgcccgctgt cctcaggatt gcgaacctga
     1501 ttttcggttt ggcaccctcc gcagcgacgc ggacgccgcc accgcgattg ggtgtggtgg
     1561 tgagcaaggc cgagaccggc gtgaagatcg atgaggtggt cacccagagc gttgccgaaa
     1621 cgacctccct tcaggcgggc gacgtgatcg agaccgcggc tggcgtgagc gtccgtcggc
     1681 cagctgacct gatcgccatt gttcagcggc aggcgccggg cacctggctg ccgctcactg
     1741 tacgcaggga tgaggcaaga ttggaaatgg tggcacgctt tccggcagaa cgatgatgct
     1801 gttgcagcga ctattgctgg cgattgggct ggcctcctcg ctcgcgccgg cctcggcggc
     1861 aggagagcct cattatatcg tccagctatc cctccaaccg gcgctgcggg agttgcatgt
     1921 tcggatgagg gtgagcttgc cggccggcgc cgaacggcgc ttccaactcg gccgcgggtt
     1981 tgcagtgaag acgatggcga ttgatgggca gacggtggac cccgtgaggc agacctggcc
     2041 cctgccgacg gggcgctctg tcgaaatcgt gtataccgcc acgctgccgg gccagggcgc
     2101 agttcaagcg attggcgagc tcgcgccgtt cgctgatccc gaaggctctt atg
//
LOCUS       DASRKK010000355         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1005048, whole genome shotgun sequence.
ACCESSION   DASRKK010000355 DASRKK010000000
VERSION     DASRKK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005048"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFC15_07750"
     CDS             <1..462
                     /locus_tag="VFC15_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM10089.1"
                     /translation="TMEEALSQSDFISIHVPLLREGESDTPTYHLINERTLRTMKPTA
                     YLVNTSRGPVVDEKALAKALKEKWIAGAALDVYETEPLPPDSPLRDPDIEDRCRLYPH
                     FASAARITRLSVDPERGMAGRCVQGLIDVLEQNYGGDITKMPYVVNKEAFR"
     gene            complement(641..1336)
                     /gene="purQ"
                     /locus_tag="VFC15_07755"
     CDS             complement(641..1336)
                     /gene="purQ"
                     /locus_tag="VFC15_07755"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZM10090.1"
                     /translation="MKFGVIIFPGSNCDHDAYWAISEVARQPVTFLWHDSPDLQGCDA
                     IIVPGGFAYGDYLRTGAIAKFSPVMESVRKFAADGGLVLGICNGFQILCESGLLPGAL
                     LRNAGLKYVCKPVHIRVESTNTLYTQQLTKGEVLEIPIGHMEGNYFCDEPTLAELKDQ
                     DRIIFRYTTPSGEVTAAANPNGSLENIAGICNAGRNVLGMMPHPERAAEAELGMTDGA
                     KIFHSLVAAMAAK"
     gene            complement(1399..1629)
                     /locus_tag="VFC15_07760"
     CDS             complement(1399..1629)
                     /locus_tag="VFC15_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10091.1"
                     /translation="MSNAQFFTALCLPLVVFIIGLYHQNTGIRDLRSELIQFRSEVNA
                     RFLSIDAKFDALTGNVIDIDKRMVRVEERLKL"
     gene            1721..2284
                     /locus_tag="VFC15_07765"
     CDS             1721..2284
                     /locus_tag="VFC15_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM10092.1"
                     /translation="MQFSRHMNITGGALRHWFIAQCYDSAAVGALWWFGLDYLHVPWA
                     PFWAMLATGLQFIPHFGPVLAFMGPALVALFAGGFDQFLYVLILYGVIIVIDGLFLQP
                     VIMRRTARVPIWASLIVPILLSLIFGFWGLLLAAPLLAVIYAYRAYGKRAEPTVPRQT
                     PSGQTITGGRLISPEGAIGRTPPDDMQ"
     gene            complement(2296..>2582)
                     /locus_tag="VFC15_07770"
     CDS             complement(2296..>2582)
                     /locus_tag="VFC15_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZM10093.1"
                     /translation="AALLGFNLRRDAFVATATAIALLVDLFRMPVYALLQFHQIVSQW
                     QLAAIATLGVIAGTVSGKWMLRRIPENTFRLIVSGIILVLGFWMLIHPAA"
BASE COUNT          546 a          739 c          761 g          536 t
ORIGIN      
        1 acgatggaag aggcgctcag ccagagcgac ttcatcagca ttcacgtgcc cctgttgcgt
       61 gagggtgaga gcgacacgcc cacgtatcat ctgatcaacg agcgcaccct gcgaacgatg
      121 aagccgacgg cttacttggt gaacacctcg cgcgggccgg tggtggacga gaaggcgctc
      181 gctaaagctc tcaaggagaa atggattgcc ggcgcggcgc tcgatgtgta tgaaaccgag
      241 ccgttgccgc cggattcacc tttgcgcgat cccgacattg aggaccgctg ccgcctgtac
      301 ccgcacttcg ccagcgccgc cagaatcacg cgcctgtccg tcgatcccga gcgcggcatg
      361 gccggacgtt gcgtgcaggg cctgatcgat gtactggagc aaaactacgg cggcgacata
      421 accaagatgc cgtacgtggt gaataaggaa gcgtttcgat gaggcagcgg ttagcaatta
      481 gcaattagca attagcaatt agcacttagc aattagtttt ccgtcaagga cacgcccatg
      541 cctctcgctg cccgatggga cacctcggtg atgcatggct tggtgatgca tcggcggtct
      601 atgagactag gcacccaagc taagtgctaa atgccaaatg ctacttcgcc gccattgccg
      661 cgaccagcga atggaagatc ttcgccccgt cggtcatgcc cagttccgct tcggcggcgc
      721 gctccggatg cggcatcatg cccaacacgt tgcgccctgc gttgcagatc ccggcaatgt
      781 tctccagcga tccattggga ttcgcggcgg cggtgacctc gcccgacggc gtcgtgtagc
      841 gaaagatgat gcgatcttgg tctttcaatt cggccagggt gggctcgtcg cagaagtagt
      901 tgccttccat gtggccgatg ggaatctcca gcacctcacc tttggtcaac tgctgcgtgt
      961 aaagcgtgtt ggtgctctcc acgcggatgt gcacgggctt gcaaacgtat ttcaatcccg
     1021 cattgcgcag cagtgcaccg ggcaatagcc ccgactcaca cagaatctga aatccgttac
     1081 agatgcccag caccagtccg ccatcggctg cgaacttgcg cacagactcc atgaccgggg
     1141 aaaacttagc gatggcgccg gtgcggaggt agtccccata agcgaatcca ccgggcacga
     1201 taatggcgtc gcaaccctgc aggtcgggcg agtcgtgcca gaggaaggtc acgggctgcc
     1261 gggccacttc cgaaattgcc caataggcat cgtggtcgca attggaaccg ggaaagatga
     1321 tcacgccaaa tttcattgcc gtaagtttct ttcgggaaag ctaaaatata gtacggttgt
     1381 gggggataac ggctcgcttt acagcttcag acgctcctcg acccgcacca tacgtttgtc
     1441 gatgtctatt acgttgccgg taagcgcgtc aaacttggcg tcaatcgaca ggaaccgcgc
     1501 attcacttcc gagcggaact gaatcagctc gctacgaagg tctcggattc cggtattctg
     1561 gtgatagaga ccgataatga acaccacgag cggcaaacac agcgcggtga agaactgagc
     1621 gttgctcacg gttcattata cctcgggctt cactcgggct gcggtaggtg cgaggtgggt
     1681 cgcgcgatgg gcctctgcac aaggcggcat caaagtagtc atgcaatttt cgcggcacat
     1741 gaacatcacc ggcggcgcgc tgcgccactg gttcatcgca caatgctatg actcggcagc
     1801 agtgggcgcg ctgtggtggt ttggactcga ttacttgcat gtgccgtggg cgccgttctg
     1861 ggcgatgctc gcgaccggcc tgcagttcat tccgcacttt ggaccagtgc tggcatttat
     1921 ggggcccgcg ttggtcgcat tgttcgctgg cggcttcgac cagtttctgt acgtcctcat
     1981 tctctacggg gtcatcattg tgattgacgg gttgttcttg cagccggtga tcatgcgacg
     2041 cacggcccgg gtgccgatct gggcatcgtt gatcgttccg attttgctca gcttgatatt
     2101 tggcttttgg ggattgctgc tggctgcccc gctgctggcg gtcatctacg cttaccgggc
     2161 gtacggcaaa cgagcggaac cgacggtccc ccgacaaact ccttccggac agacaatcac
     2221 cgggggccgc ctgatctctc ccgaaggtgc aatcggacgc acacctcctg acgacatgca
     2281 ataattcgcc ttccgctagg cagcgggatg gatcaacatc cagaagccca gaaccagaat
     2341 gatgcccgag acgatcagcc ggaaagtatt ctccggaatg cggcgtaaca tccactttcc
     2401 ggagaccgtg ccggcgatca ctcctaatgt ggcgatggcc gcaagctgcc attgggaaac
     2461 gatctgatga aattgcaaca gggcataaac gggcatgcgg aagaggtcaa cgagtaaagc
     2521 gatcgcagta gcggtcgcaa caaatgcatc gcgtcgcaga ttgaatccca gcagggccgc
     2581 cg
//
LOCUS       DASRKK010000356         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_906999, whole genome shotgun sequence.
ACCESSION   DASRKK010000356 DASRKK010000000
VERSION     DASRKK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906999"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1849)
                     /locus_tag="VFC15_07775"
     CDS             complement(194..1849)
                     /locus_tag="VFC15_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM10094.1"
                     /translation="MYFRVKRTGSYAYLQIVESFREKGQVRQRVLSTVGRLDTLQASG
                     QLDTLMRSGLRFCEKLTVIDAHAAGQTEPVQVQRVGPDLVFSRLWEALELGTILKRAL
                     QSRRYEFDVERAVYLTVVHRLFASGSDRAAERWRQAYRLPGTETLELHHLYRAMAFLG
                     EPLKDQPGARVLGTPRCTKDWIEEELFEQRRDLFSQIDLVFFDTTSIYFEGEGGQEIG
                     RHGKSKDHRPDLKQMVVGLALDLHGWPLCCELWPGNTADVTTLVPVVNRLRQRFRVRR
                     VCMVADRGMISAGTIRALESEELDCDYILGARMRSVKEVSDRVLADRGRYQEITPERE
                     TAKDPSPLKVKEVLVEDRRYVVCLNEEQRRKDAADRKAIVEHLREQLKQGDKELVGNK
                     GYRKYLLAGAGERFSIDEAKIKEEARYDGKWVLQTNLADEPEIIALAYKELWMVEDLF
                     RTMKSILETRPIYHKRDETIRGHVFCSFLALLLRRALEQRLEEKGETWEWAEIVRGLD
                     NLQEVEALFQGKRFVLRSQVLGQAHKALMAAGVALPPTLREKT"
     gene            complement(1922..>2177)
                     /locus_tag="VFC15_07780"
     CDS             complement(1922..>2177)
                     /locus_tag="VFC15_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10095.1"
                     /translation="YYLIGVRISTGKTAVQHSLWADTVEDEEKIWREFLAILETVEKP
                     VLIHYGSSSTVRTLPEDEFSRNAGFIMHVSRPTSKCSGKF"
BASE COUNT          467 a          690 c          541 g          479 t
ORIGIN      
        1 gttgaatcct agaacttcac tctgtagaaa gtcagcgtag gccccggcgt gacgcggtaa
       61 taaggcgcag tagcgtcagg aaccgggtta tactcatctt cgacagtttg aaaagctagc
      121 tccctgattt tgaaggactt acaaagacgc caaatagtct tggcactaca tttctggttc
      181 cctaagtttt gattcaggtc ttctcccgca aggtcggtgg cagggcgact cccgctgcca
      241 tcaacgcctt gtgcgcctgc cccaacacct gactgcgtaa cacaaagcgc ttgccctgaa
      301 acagggcctc gacttcttgg agattatcca aaccgcgcac gatctccgcc cactcccagg
      361 tttcgccttt ttcctccagg cgctgctcca gcgcgcgcct gagcaacaac gccaggaaac
      421 tgcagaacac gtggccacga atcgtctcgt cgcgcttgtg ataaatggga cgtgtttcca
      481 aaatcgattt catcgtgcgg aacaggtcct ccaccatcca cagttcttta taggccaaag
      541 cgatgatctc gggttcgtcg gccaggttgg tctgcaaaac ccatttaccg tcgtagcggg
      601 cttcttcctt gatcttcgcc tcgtcaatgg aaaagcgctc ccccgccccc gccaggagat
      661 acttccgata ccctttgttg ccgaccaact ccttgtcccc ctgcttcaac tgttcccgca
      721 agtgctcgac aatggccttc cggtcggcgg cgtccttgcg ccgttgttct tcattcaggc
      781 agaccacata gcggcggtct tccacgagca cttctttgac tttcaacggc gagggatctt
      841 tggccgtttc ccgctcggga gtgatctcct gatagcgacc gcggtcggcc aagacgcgat
      901 cgctcacctc ctttaccgag cgcatccgtg cccctaaaat gtaatcacag tccaactcct
      961 cagattctaa cgccctaatc gtacccgcac tgatcatgcc ccggtccgcc accatgcaaa
     1021 cccggcgcac ccgaaaccgt tgtcgcaatc gattgaccac cggcacgagc gtagtgacat
     1081 cggccgtatt gccgggccac aattcacagc acaagggcca accgtgcaga tccagcgcta
     1141 accctaccac catctgcttg agatccggcc gatggtcttt gctcttgccg tgccgtccaa
     1201 tctcctggcc gccctcgcct tcgaaataaa tggacgtggt gtcgaagaag accagatcaa
     1261 tctggctgaa taaatcccgc cgttgctcaa agagttcctc ctcgatccaa tctttggtgc
     1321 agcgcggcgt gcccaaaact cgagcgccgg gctggtcttt gagcggttcg cccaggaacg
     1381 ccatggcgcg atagagatgg tgtaactcca gagtctcagt gcccggcaac cgatacgctt
     1441 ggcgccatcg ctccgcggct cgatcactac ccgacgcaaa caagcggtgc accacggtca
     1501 aatagacagc ccgttcaacg tcgaactcgt agcggcgcga ttgcagcgca cgtttgagga
     1561 tcgttcccag ctccaaggct tcccacaagc gactaaagac caggtccgga ccgacccgct
     1621 gcacctgcac cggttcggtc tggccggcgg catgcgcatc gatgaccgtc aatttctcgc
     1681 agaagcgaag acccgaacgc atcagcgtat ccaactgtcc cgaggcttgg agcgtgtcca
     1741 accgcccaac cgtgctcaac acgcgctggc gcacctggcc cttctcgcga aagctttcga
     1801 cgatttgtag gtaggcgtag gagccagtgc gtttcactcg aaaatacata gggcggtttc
     1861 ttttcctcta ctggcatgaa ctcagtgctc ctcagttgca atataaaacc gaagaacgcc
     1921 ctcagaattt gccactacat tttgaagtcg gacgactcac gtgcatgata aacccagcat
     1981 tcctgctgaa ttcatcttca ggcagcgtcc ggactgtaga agacgagcca tagtgaatca
     2041 gcaccggctt ctcaacggtt tcgaggatgg caaggaattc tcgccaaatc ttttcctcgt
     2101 cctcgacggt gtccgcccaa aggctgtgtt gaacggcggt tttgcccgtg ctgatgcgca
     2161 cgccgatgag gtagtag
//
LOCUS       DASRKK010000357         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1297794, whole genome shotgun sequence.
ACCESSION   DASRKK010000357 DASRKK010000000
VERSION     DASRKK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297794"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFC15_07785"
     CDS             complement(<1..313)
                     /locus_tag="VFC15_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10096.1"
                     /translation="MTDSSIEAKLQTIDDELANAGVPLRYRPLECFKRLYGAAEEGRH
                     EQLFDPLVQWYLTRYGDEARWNGVIGRCPVLIRGKVFLGVARFSAASETVVNWRDHVE
                     EL"
     gene            complement(394..618)
                     /locus_tag="VFC15_07790"
     CDS             complement(394..618)
                     /locus_tag="VFC15_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10097.1"
                     /translation="MRPEYDLARLAGGVHGKYYRQAVAGTNLILIEPELARVFRDSES
                     VNRALRLLLETAAASATAARSRHRAPKKRS"
     gene            complement(623..913)
                     /locus_tag="VFC15_07795"
     CDS             complement(623..913)
                     /locus_tag="VFC15_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM10098.1"
                     /translation="MVYEWNAAKAKANAHKPGVSFEEASTVFLDPLAWTFSDPDHSAA
                     EAREITIGYTVRHRVVFVAHCQRGGRTRLIGARKATRKERKQYEEGIGKQEK"
     gene            978..1502
                     /locus_tag="VFC15_07800"
     CDS             978..1502
                     /locus_tag="VFC15_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10099.1"
                     /translation="MSTRNVSGLVAIALWASLCMGQEVHVTRGKGVVVLSVSAQKPYQ
                     LLSGEGKMPMLSVECAHKGKKAGHLVVFSPGGSLVEENFEVDAKPGQLTFNMTIGGTK
                     QITAWVPYGDTVTFTYFGKSEPERMKFIQSLLSSGSVSIEFKPFLTGVPTTSVFDLSK
                     LGAEMDKYPECATK"
     gene            2140..2802
                     /locus_tag="VFC15_07805"
     CDS             2140..2802
                     /locus_tag="VFC15_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZM10100.1"
                     /translation="MTSLVPALKNGLRSLVAIRQLNLSRKAALLTFDDGPHPEVTQKV
                     LNLLRKYSARAVFFVVGSRIPCAPHLLQEILAEGHIIGNHTFHHRARGLSYVACLQDT
                     RQCQSEVLRLTGFAPTLFRPAEGVITPANLLACRRLRMRTILWSYDTNDWRLSSDAEV
                     REQTERALGALATGARNPIILMHDDNRYSPGLLEPLLECLSSRGYDLKTGAGSLDSRA
                     SV"
     gene            2861..4189
                     /locus_tag="VFC15_07810"
     CDS             2861..4189
                     /locus_tag="VFC15_07810"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM10101.1"
                     /translation="MKPDAADNSVPGEIGALLSSERVILRETQRAVTPLGGVAVFVVF
                     LHKLGLVEKLRQHMPIQWKSPNYIDPTTTFTAFLMAVLAGAKRFAHAHWLRNDRALHA
                     LLGLSRFPTDDTIRNLFRRFGLGDVHRLFDPLAEWQMERLPQRSDGYSLDLDSTVFER
                     YGQQQGSLQGHNPRKHGRPSHHPLLAVLAEAHFLLHGWLRSGNCGAARGVVEFLQEAL
                     ALWGQRQKIRLVRADSGFFEDNLLSFLEQRSLSYIVVARLTKWIKREAQRVQVWQALD
                     ENYSVGEFRVQLLDWERERRFVVVHELVREGRNNVGRKLLDVPGYTFRLFVTSRSDTP
                     EEIWRDYNRRADMENRIAQLKHDLGADGFCLQEFFATEAAFQAVLLLFNLLAEFQRAA
                     GLPVYRQPATLRAQVFTCGAILGRAGRRLVLHLSQSWADCKHATHCWTKS"
     gene            complement(4331..5428)
                     /locus_tag="VFC15_07815"
     CDS             complement(4331..5428)
                     /locus_tag="VFC15_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10102.1"
                     /translation="MRGDAAVQVRPATVEDAKGIAAVRARNGMPSFDIEDWRQSWESH
                     PFRDEFAETPFGWVLENGREGIVGTLSNIPMMYDLDGKPVRASASGAWAVDSPHRRSS
                     LLLMSALLRQKGVDLHIIGSANTVASPIMTGLKLPRIPAPDFNVSLLWVTGYRGFATS
                     VLRKKNIPAASWLGMPAGAALGAYDCLKGRVGRRRVEVRQYDSFTEDFDIFWNELRRR
                     PAGRLRAARTSAALRWRFRQSLRTGKLAILGLTDSQGALSGYCIALEQQREHIGLKQY
                     LVADLQSLDDAPGTLLELLEAAIDLSRRRGIHLVEWQGGNRAKRYVALSLRPRPYLYP
                     SWPAFYRLFRADLGAALENEATWDLLPIDVY"
     gene            complement(5638..6099)
                     /locus_tag="VFC15_07820"
     CDS             complement(5638..6099)
                     /locus_tag="VFC15_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZM10103.1"
                     /translation="MASLDPKFHEETVSIHKEHEELVAQLDQMDAALEQIVCYSEIFT
                     DLATANQALSHGKWMAEWLPRHYEHEETTVLATIAKMSPELASFAREMKRQHNEMRVR
                     LENFRDRIEHLSETSDLEDAVNQIKKDGTNLTRMMRLHMAAEEKKFASVEN"
BASE COUNT         1244 a         1906 c         1814 g         1279 t
ORIGIN      
        1 gcagttcctc gacgtgatcc cgccagttca ctaccgtttc tgaggccgcg ctgaaccgcg
       61 ccacacccaa gaacaccttc ccgcgaatta aaacggggca gcgaccgatg acaccattcc
      121 agcgggcttc gtcgccatac ctcgtcagat accactgcac tagcggatca aaaagttgtt
      181 cgtggcgtcc ctcctccgcc gcaccataca gccttttgaa gcattccaaa ggtcggtatc
      241 gaagaggtac accggcgttt gccagctcat catcgatcgt ctgcaacttg gcctctatcg
      301 aactgtcagt catcgcatca tccactagaa ttatctgcgc caaacggcag ggtataaatg
      361 ggagtgcgct ggtggcccag cctactagcg tggttacgag cgtttcttcg gtgcgcgatg
      421 ccgcgaacgg gcggcagtag cgctcgcggc tgcagtttcc agcaggagcc gcagggcgcg
      481 gtttaccgat tccgaatcgc gaaacacgcg agcgagctcc ggctcgatga gaatcagatt
      541 cgtccctgcg acggcctggc ggtagtactt gccgtggacc cctcccgcga gtcgggcaag
      601 atcgtactcc ggacgcaagt cgtcatttct cctgtttgcc gatgccttct tcatactgtt
      661 tgcgctcttt cctggtcgcc tttcgggcac cgataagtcg ggtgcggccg ccacgctggc
      721 aatgcgctac aaagacgacc cgatggcgga cggtataccc gattgtgatc tccctcgcct
      781 cagcagcaga atgatccgga tcggaaaacg tccaggccaa agggtcgagg aagaccgtcg
      841 atgcctcttc aaaggataca ccgggcttgt gggcattggc ttttgccttc gccgcattcc
      901 actcgtagac cacccgctca gtttagctca acctgggcgg tggcgcgggg cgggtgccga
      961 gtagtatgtt ggtgagcatg tccacaagaa acgtatccgg gttggtcgcg atcgcgttgt
     1021 gggcgtcgct atgcatgggg caagaggtgc atgtcacccg gggcaagggc gttgttgttc
     1081 tgagcgtttc ggcgcagaag ccctaccaat tgctctcggg tgagggaaag atgcccatgt
     1141 tgagcgtgga gtgcgcgcac aagggcaaaa aggccggaca ccttgtggta ttttcgcctg
     1201 gcggttccct ggttgaagaa aatttcgaag tcgacgcgaa acctggacag ctgactttca
     1261 atatgacgat aggcgggacc aagcaaatta cggcgtgggt cccctacggc gacaccgtaa
     1321 cctttaccta tttcggcaag agtgaacctg agcgtatgaa attcatccag tcgttgctca
     1381 gctccggatc ggtttccatc gagttcaagc ccttcctcac gggtgtgcca accaccagcg
     1441 tttttgacct aagcaaactc ggcgctgaaa tggacaaata tcccgagtgc gctacgaaat
     1501 agttctatgc cggcaacgcc ccgccaaatc caagaaggtc gcataacgcg ccttggtggg
     1561 cggcccggcc aagcacactc cagtcgtcga aagaccttta gatctggggt gccccgttct
     1621 agcccggttt tggctagggc gggcagtcca ggaattgcga gacttttgta gactgcggtc
     1681 gcgctcgcag atgccactca tcactgacaa tcttcgcccc cactgcctac tgtcgaacag
     1741 gacatgtgga gaccgagtct ccctggacac gcagaaaacg tgagcggaca ggaacgattc
     1801 cgaaggcatc aatgagcaaa ccgtcaactg ccccgcccca gccaaaaccg ggctagaacg
     1861 gggcaccccg gtttgtggag gagcactcgg atggggcacc cggcagaagc gccattcggg
     1921 cgtcggaaag ttctgcagcg ataaactatc ctgcgcgcac ggttgacgca cttattcagc
     1981 agctactgag aagatcggat aaagtgaatt gacgcgtcca tacggaactc cctggagatg
     2041 ggttcctccg accctgcgaa gttgagaagc ccggtcgggg agtctccgcc ggaggttcag
     2101 cagctacggt acgatgaatg tttgttccca gaggggctca tgacgtcgct cgttcccgct
     2161 ctcaagaatg gtctacgcag tctcgtcgcg atccgccaat tgaacttgtc ccggaaagca
     2221 gcgctgctca ctttcgacga tggccctcac cctgaggtca ctcagaaggt gctgaacctg
     2281 ctccgcaagt attctgcacg ggcagttttt ttcgtggtcg ggtcgcgaat tccgtgtgct
     2341 ccgcatctgc tgcaggagat cctggccgag ggccacatca tcgggaacca caccttccac
     2401 catcgggcga gaggcctctc ctacgtagcg tgtctgcagg atactcgcca gtgccagagc
     2461 gaggttctgc gtctcactgg attcgcgccg acgttatttc gcccggcgga aggcgtgatt
     2521 acgccagcca accttcttgc ctgccgacgg cttcgcatgc ggacgattct gtggagttac
     2581 gataccaacg actggcggct atcctcggac gctgaggtgc gggaacaaac ggaaagagcc
     2641 ctcggtgcac ttgcaaccgg cgcgcgcaac cccatcatcc tgatgcacga cgacaaccgc
     2701 tattcccctg gcttactcga gccgcttcta gagtgcctct cgagcagggg ctacgatctc
     2761 aaaacgggcg cagggtcgct ggattcacgg gcgagcgtat aagcctgaat tccgaagctc
     2821 cgcgttttgt tttttggtag acatctgaga caacagggag atgaaaccag atgcagcgga
     2881 caattccgtc ccgggcgaga tcggcgccct gctgtcgagt gagcgggtga ttctgcgcga
     2941 gacgcagcgt gccgtgaccc cgctgggcgg ggttgcggtt tttgtggttt tccttcacaa
     3001 gctggggttg gtcgagaagc ttcggcagca catgccgatc cagtggaagt ccccgaacta
     3061 catcgatccc accacgacct tcaccgcgtt tctcatggcc gtgttggcgg gagccaaacg
     3121 ttttgctcac gcccactggt tacgcaacga tcgcgccttg catgccttgc tgggcctgtc
     3181 ccgctttccc actgacgaca ccatccgcaa tctgttccgc cgttttggcc tgggcgacgt
     3241 gcatcgtctc ttcgacccgc tggccgagtg gcagatggag cgcttgccgc aacgcagcga
     3301 cggttacagc ctggacttgg attccaccgt cttcgaacgc tatggccagc agcaaggctc
     3361 gttgcaaggg cacaatccgc gcaagcacgg acgccccagt catcatccct tgctggcggt
     3421 gctggccgaa gcccacttcc tgctccacgg ctggctgcgc agcggcaact gcggagccgc
     3481 gcgtggggtg gtggagtttc tccaagaagc cctggcgctg tggggacagc gccagaagat
     3541 ccgcctggtg cgagcggatt ccggcttctt cgaggacaac ctgctgagct ttttagaaca
     3601 gcgctcgctg tcctacattg tggtagcccg gctgaccaaa tggatcaagc gcgaagccca
     3661 gcgggtccag gtttggcagg cgctggacga aaactattcc gtcggcgagt tccgcgtgca
     3721 gctgctggac tgggagcgcg aacggcgctt tgtggtagtg cacgaactgg tgcgcgaagg
     3781 ccgtaacaac gtaggccgca aactgctgga cgtgcccggc tacacctttc gcctcttcgt
     3841 caccagccgc agcgataccc cagaagaaat ctggcgcgac tacaatcggc gggccgacat
     3901 ggaaaaccgt atcgcccagc tcaagcatga cctgggcgcc gacggtttct gtctgcaaga
     3961 attcttcgcc accgaagccg ccttccaggc cgtcttgctg ctgttcaacc tgcttgcgga
     4021 gtttcagcgc gccgccgggc tgccggtcta ccgccaaccc gcgacgctgc gcgcccaagt
     4081 gttcacctgt ggcgccatcc tgggccgggc ggggcggcga ttggttctcc acttgtcgca
     4141 aagttgggcg gattgcaaac acgcaaccca ttgctggaca aaatcttaag ctgggaaatc
     4201 ccaacttcgc cgaagttgga tgttgctatc gccacctgat tcagaaatca ccactcaccg
     4261 gcgatcacac tcgcggaaaa agagggactt aagctcaact tcgggattca ggataagaac
     4321 atccagcggc tcagtatacg tcgatcggca gcaagtccca agtagcttcg ttctccagag
     4381 cagcgccaag gtccgcgcgg aacagcctgt aaaaagccgg ccaggaagga taaaggtagg
     4441 ggcgcggccg gagagaaagc gctacataac gtttggcacg attgccacct tgccactcca
     4501 ccagatggat gccacgcctt cggctgagat cgatggctgc ttcgagtaac tccagcaagg
     4561 taccgggcgc gtcgtccaga ctctggaggt cggctaccag gtactgtttc aggccgatgt
     4621 gctcccgttg ttgctccagc gcgatgcagt agccggaaag ggctccctgc gaatccgtca
     4681 acccgagtat ggcgagtttg cccgttcgca gcgattgccg gaagcgccac cgcagcgcgg
     4741 ccgaagtccg ggcggctcgg agccgcccgg ccgggcggcg gcggagctcg ttccaaaaga
     4801 tgtcgaaatc ctcggtgaac gagtcgtact ggcgcacctc aacgcgcctc cgcccaaccc
     4861 ggcccttgag acagtcgtag gcgcccagcg ccgcgcccgc cggcattccc agccaggatg
     4921 ccgccggtat gttcttcttg cgcagcacgg aagtcgcgaa gccgcggtag ccggtgaccc
     4981 agagcaatga cacgttaaag tcgggcgcgg ggatgcgcgg tagctttagg ccggtcatga
     5041 tcggagaggc aaccgtattg gcgctcccaa tgatgtgcag gtcgacgccc ttctgccgca
     5101 gcaatgccga catgagcagc agcgaactgc ggcggtgggg cgaatccaca gcccaggctc
     5161 cggaggcgct ggctctaacg ggcttgccgt ccaagtcgta catcatgggg atgttggaca
     5221 atgtgcccac gataccttcc cgaccgttct ccagcaccca tccgaacgga gtttcggcaa
     5281 actcatcgcg aaaggggtgg gactcccacg attgccgcca gtcttcgata tcgaagctgg
     5341 gcatgccatt acgagcccgc accgcggcta tccccttcgc atcttccaca gttgccggac
     5401 gaacctgcac cgccgcgtct cctctcatcg gctacatcgg accgcacgac gccaaccgcc
     5461 gactcaagac caattctaag ggatgacggc gaaatgcacg tgacgacaga attcctgaag
     5521 aagatcgtag ccgtcccaga agcgatgaaa ctggcgggtc cacccaagcg ctcataaagt
     5581 tgacggtgcg gcagtttgat cgtggccgcc tttttgtttc gtaactcaac ttcgccgtca
     5641 gttctccacg ctggcgaact ttttctcttc cgctgccatg tgcagccgca tcatgcgagt
     5701 gaggttcgta ccgtctttct ttatctggtt gaccgcatct tccaggtcgc tggtctcgct
     5761 caggtgctcg atcctatcgc ggaaattctc cagccgcact cgcatttcgt tgtgctgccg
     5821 cttcatctcg cgagcgaacg aggccagctc cggactcatc ttcgcgatgg ttgccagcac
     5881 cgtggtctct tcatgctcgt agtgccgggg aagccactcc gccatccact tgccgtggct
     5941 cagggcctgg ttggcggtgg caagatcggt gaaaatctcc gagtagcaga caatctgttc
     6001 cagagcggca tccatctggt ccaactgcgc caccagttcc tcgtgttctt tatgaatgct
     6061 gactgtctct tcgtggaatt tgggatccaa gcttgccata tcacacctcg ccagcttgat
     6121 ggtcgtcccg cccctgcgcc cgcggcagtg accgaaatca cttactggcg tgacgttcgg
     6181 cggtcatccc gcaataaaca agaccggagc tggcagcccc ggccggttgt ttttgcgctt
     6241 att
//
LOCUS       DASRKK010000358         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_809462, whole genome shotgun sequence.
ACCESSION   DASRKK010000358 DASRKK010000000
VERSION     DASRKK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809462"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(784..1299)
                     /locus_tag="VFC15_07825"
     CDS             complement(784..1299)
                     /locus_tag="VFC15_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM10104.1"
                     /translation="MKTTFHTARWAALAAGLLISVSAVHSQDKSKIPSRDLKETKTMT
                     GYVGPIEKQTMENEYFRQVLFTGPHSQLVVMCLQPGEDIGDEVHPHVDQFFRIEKGQA
                     KFVLNEKEEHMVHDGDAVVVPAGTYHNVINTSKTLELKLYTIYSPPNHPDGTVHKTKA
                     DAVAAEALKHK"
     gene            complement(1371..1700)
                     /locus_tag="VFC15_07830"
     CDS             complement(1371..1700)
                     /locus_tag="VFC15_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10105.1"
                     /translation="MPKLVEQGIAVLGMKSMGNGNLLKSATVTPVECLHYALNLPVSV
                     VITECESMERLDQALEAARTFRPLDKARISALLTKMRSAALTGKYGPFKTTAKFDGTA
                     NNPHWMS"
     gene            complement(1862..>2120)
                     /locus_tag="VFC15_07835"
     CDS             complement(1862..>2120)
                     /locus_tag="VFC15_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HZM10106.1"
                     /translation="VNLLVNYGWEWDLAGLKNGGLRSGEVSRLDLIVRWGGRRRLSGF
                     LPVQSIYADFYVRDEYWPDFDLQHFDHALAWFRNQDPTLGG"
BASE COUNT          416 a          640 c          573 g          491 t
ORIGIN      
        1 catgatggtc gaaaagctct cgtgaatgtc gaccggcatg taactcgaag gtattttgct
       61 gttgtcctgt gagtgcaccg cagaaacgct tatcagcaag ccggcggcta gtgccgccca
      121 tcttgatgtg cggaatgtcg ttttcattgt ttgaacgagg acctccaact gcccacatcg
      181 cacgtttctg gccattcctg gccactcaag gaacaactca aacgatccac ccgggattat
      241 cctgaatttc gaagttggaa cctcagccca catccactgg gtgacccgtc gcttacgctc
      301 cgggttccga acaaaccaca gcggccgctc cagcaggctc ggagcccgga gcgcaagcga
      361 cgggtcagcg tcgcaaatgc aacgaaccct tctgccggtt gcgtgcccgc cgcaacctcg
      421 ctgctgccat cggctcgacg ccgtctgcca cactgaactt cggaattcag gactagtggc
      481 cggcaccgtc aaacttcgct gtcgtcttga agggttcgca ctttcccgtc aggcggtcgt
      541 cccgtgagca aagcggatac tcgggcctgc atccgttggc tggaatgtgc gcgcggcttc
      601 cattgccggg gctttggagc gggagcgggc aaccgctccc gctcggcgcg cgggatgctg
      661 ctgctcccgt gggccatctc cggatcgtgt gccgactgac ggaggcttcc acacgcagca
      721 ctgttcgctc gtggccgatg agtgaagccg aatccgagcg ccgcgttgac cggatgttgc
      781 gccttacttg tgttttagtg cttcggcggc taccgcgtcg gccttggtct tgtggaccgt
      841 gccatccggg tggttcggcg gagagtagat cgtatatagc ttcaactcga gcgtcttcga
      901 cgtattgatc acgttatggt acgtccccgc cggaaccacc acagcatcgc cgtcgtgcac
      961 catatgttct tccttctcgt tcaggacgaa tttcgcctgc cccttttcga tgcggaagaa
     1021 ctggtcgaca tgcgggtgca cctcgtcgcc aatgtcttcg cccggttgca ggcacatcac
     1081 aacaagctgc gaatgcggac cggtgaatag cacctgccgg aaatactcat tctccatggt
     1141 ctgcttttcg atcggcccaa cgtatccagt catagttttg gtctccttca ggtccctcga
     1201 aggtattttg ctcttgtcct gtgagtgcac cgcagaaacg ctgatcagca agccggcggc
     1261 tagtgcggcc catcttgctg tgtggaatgt cgttttcatt gtttgatcga gaacctccaa
     1321 ctgcccacag tgcacgtttc tggccattcc tggccactca acgaacaacc tcaactcatc
     1381 caatggggat tattggcggt gccgtcaaac ttcgcagtgg tcttgaaggg tccgtacttt
     1441 cccgtcaagg cggcagacct catcttcgtg agcagagcgg atattcgggc cttatccagc
     1501 ggccggaatg tgcgcgcggc ttccagcgcc tgatcgagcc gttccatgct ctcgcattcg
     1561 gtgatcacca cggaaaccgg cagattcagc gcgtagtgca ggcactcgac gggtgtgacc
     1621 gttgcgctct tgagcaggtt tccgttcccc atcgatttca tgccgagcac ggcgattccc
     1681 tgctcgacga gtttgggcat tacctgttgc gcaaagctgc ggaaggggta atccctgcag
     1741 ttgtcccctg aaggtgatgc cgcggtcgat cgccgtgaag gtcaaaaggc aatgcgatag
     1801 gattagcttt ttccaacgtt tccggctact gccttaaacc agcatacgct ataccactcc
     1861 atcacccgcc gagtgtcggg tcctggtttc taaaccaggc gagtgcgtgg tcaaaatgct
     1921 gtagatcgaa atctggccaa tattcatccc ggacatagaa atccgcataa atcgattgta
     1981 ccggaagaaa accgcttaat cggcgtcttc ctccccagcg tacgatcaaa tccagacgcg
     2041 aaacctcacc tgaacgcagg ccgccgtttt taagaccggc cagatcccat tcccagccat
     2101 agttgaccag tagattcacc
//
LOCUS       DASRKK010000359        19196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1297932, whole genome shotgun sequence.
ACCESSION   DASRKK010000359 DASRKK010000000
VERSION     DASRKK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19196
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297932"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFC15_07840"
     CDS             <1..255
                     /locus_tag="VFC15_07840"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZM10107.1"
                     /translation="EEHGMVGRRYAGKVQQIITGSIGFDDWEWGVDLFAPDPLVFKKL
                     IYEMRFDHVSAVYAAFGQFFVGLRVPVAKLSEWTSGELPK"
     gene            444..1019
                     /locus_tag="VFC15_07845"
     CDS             444..1019
                     /locus_tag="VFC15_07845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZM10108.1"
                     /translation="MAIESACIPLPSEVIMPFSGALIVPAVASLYHRDPFSLFWVATF
                     GAIGCNLGSVLAYEIGYFGGRPLVERFGRYVFLNKRELDIADRFFERRGAIAVLIARL
                     LPVIRTFIALPAGIARMPRLKFHVYTFLGSWPWCFALAYVGMRLGEQWDKDPRIKMWF
                     HRMDGVILAVLIAGIAYFVWSHVRDRVREAA"
     gene            complement(1151..2005)
                     /locus_tag="VFC15_07850"
     CDS             complement(1151..2005)
                     /locus_tag="VFC15_07850"
                     /inference="COORDINATES: protein motif:HMM:NF005372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM10109.1"
                     /translation="MSIQKIAVVTGSSSGIGLLTTIEFARNGYQVVATMRDLGRAGRL
                     EETAQKAGVRDRLDLRRLDVTEFDSLPGAIDDIVRAHGRIDVLVNNAGFSVAGFGEDL
                     TLGEIRLQFETNFFGNVAMTKAVLPVMRQQNSGHIVQVASVAGRLGQPLLGAYSSSKF
                     ALEGFSESLRIETHSLGIRVVLVEPGAFDTDIWTRNVVIGQGAIDPNSPNKERSQRFT
                     EFVKQSAKNRRDAREIAQLILRIANDPNPKLRYLIGTDAKIQVWLRRILPWKSYERMI
                     AKAVKIDQ"
     gene            2140..3492
                     /locus_tag="VFC15_07855"
     CDS             2140..3492
                     /locus_tag="VFC15_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZM10110.1"
                     /translation="MPTGKTQDKSKTVKGAVAPAKGKLGIMIPGMGAVATTFVAGVEA
                     VRKGIASPIGSLTQMGTIRLGKRTDGRSPKVKDFVPLAGLNDLVFTGWDIFEDDMYAA
                     AGKAGVLERSLLEQVKPFLSSIKPRKAVFDKNYVKKLDGPNVKKGKNKKELCEQLRED
                     IRDFKQSSKTERQVMIWCGSTESFIQAGPVHQTVKSFEKGLLANDENIPPSMIYAYAA
                     MQEGVPFANGAPNLTVDVPVIQQLSRQNEAPICGKDYKTGQTLLKTILAPGFKARMLG
                     LSGWFSTNILGNRDGEVLEDPGSFKTKEESKLSVLEHILQPELYPELYGHIYHKVRIN
                     YYPPRGDNKEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLVLLLDLAKRSSE
                     LRGIGIQEWLSFYFKSPMTAPGLYPEHDLFIQSMKLKNTMRHLMGEEMITHLGLEYYD
                     "
     gene            3833..4132
                     /locus_tag="VFC15_07860"
     CDS             3833..4132
                     /locus_tag="VFC15_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM10111.1"
                     /translation="MAARRVEYHEGAVADVKSAVLWYQERSPKAAMDFIGELHRAVDT
                     IREAPNRWPIGRNNTRRFLLLRFPFLVIYSEVESVITIWAVAHGNRRPEYWARRL"
     gene            complement(4204..4866)
                     /locus_tag="VFC15_07865"
     CDS             complement(4204..4866)
                     /locus_tag="VFC15_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HZM10112.1"
                     /translation="MIRVITIEREYGSGGAEIAKRIADRLGWKLWDQLLTNEIARLME
                     CECRVVEQHEEKRDPLYHRLLMAFMRGSFEGSLNAPRLKMADTDCVREVAERVVKRAA
                     EEGQSVIVGRGSAHYLQNRRDAFHVFVYAPLSEKVRRLKAGGKSESEAVQLAETVDRD
                     RADFIRQYFGIEWPGRHRFDLMINSKVGEETAVETILDCIKLFEKSRTKSATTALQPT
                     GV"
     gene            complement(4944..6587)
                     /locus_tag="VFC15_07870"
     CDS             complement(4944..6587)
                     /locus_tag="VFC15_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZM10113.1"
                     /translation="MTDSAANLEHPPPGATTTANAPVSEASATWRPKFNPWLIGVVVA
                     VAAFMEVLDTSIANVALPYIGGNLGASTDQSTWVLTSYLVSNAVVLPISGWLASVLGR
                     KRFFMTCLAIFTVSSLLCGFAPSLGAIILFRILQGAGGGGLQPMAQAILADTFPPEKR
                     GLAFALYGVTVIVAPTIGPTLGGWITDNYTWRWIFFINLPIGILALFLVYRLIDDPPW
                     AKRKAGAASRIDYVGMSLLVLGVGALQIMLDKGQEDDWFGSHFILTLAVLAVVGLVSL
                     VVWEWFYKNPVIEVRLFRNLNFLAANGMMFILGLMLFASLVMMPLFLQSLLGYTAESA
                     GLVLSGGGLLLLFMMPMIGVLSSKVQARYLIAFGWFALSIGMYYSSRELNLGISFRSA
                     SMIRLVQVFGLGFLFVPINLASYVGMPTEKSSSVAGLVNFMRNIGSSVGTSMVTTVIA
                     RRAQFHQVHLVANVAPGQLSFSQAAEALTARIFASGVDAAGATRQAYAIIYRTVIVQA
                     TTLAYVDTYLVLAVMAATMFVLSFALRKNDPGARRVVVD"
     gene            6764..8230
                     /locus_tag="VFC15_07875"
     CDS             6764..8230
                     /locus_tag="VFC15_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM10114.1"
                     /translation="MSTRNRLCLVALAIVLIAGLGHSQNSPVMLEALKPDSPLQENLR
                     KLTDEIGGRVPGTPAFTAAVLWGIAGFKAAGADSVHVEEFTIAQSWAEGATQVEVVSP
                     VKWRLLAHSIAWGPALKPTTARVVDVGMGKPEDFAKAGDISGAIVLAHSVVLKTWDDL
                     FDEYFRAPGIIQQAVKGKAIAIAFESSREHDILYRHLNSQTGHIDVIPQVLLAREDAE
                     RVARLIAHGEKIEMSVSMPNVIGPTIATSNVIADIKGTELPDEVVMLGAHLDSWELGT
                     GALDNGCNAALVIDTLRAIKAAGVRPRRTIRFVLYSGEEQGLLGSWAHVRAHQKDLDN
                     IVAELVLDEGSGPITGFSTGGRKDIDAAVKPMVQPFAQWKAAELTNDAMTGTDHYDFM
                     IEGVPTLVANQAEANYLVNYHATSDTYDKVDFAQLKKNEAMAAELMVQMANAPQRIGP
                     RWTRAQIEASFAETHLDDQMKGFGMWDDWQSGTRGRKQ"
     gene            complement(8309..9073)
                     /locus_tag="VFC15_07880"
     CDS             complement(8309..9073)
                     /locus_tag="VFC15_07880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM10115.1"
                     /translation="MRNFVTAILCLGLAGFAIAQSSSSATPKSTAAKPAATKPATPAA
                     ATSTAPVQAVFETTMGNITCTLFPDKAPVTVDNFIGLATGKKDWKNPASGVTMHNKPL
                     YDGTIFHRVIPNFMIQGGDPLGNGSGGPGYDFKDEFTDLTFDQPGRLAMANSGPHTNG
                     SQFFITEVPTPHLNGKHTIFGQCDNPELVKQITRLATDPRNDRPFNPPKITHVKIIDP
                     RHPAAAPAKKTTITHAHKSTTGTAAPKKSTPAPPPQ"
     gene            complement(9183..10694)
                     /locus_tag="VFC15_07885"
     CDS             complement(9183..10694)
                     /locus_tag="VFC15_07885"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZM10116.1"
                     /translation="MRASVNGGALFTVRLNLVSDVPYRATLFGAATGARRSVWLFPII
                     YNHRETISSESSNFMPMIRVVASILLLSTFSSAQTATPTTPNPSKSHSHAITISPNGL
                     ATGWQAIHNSALIIDTHADTPGRFVDENFDLAQNAGKGYMDFNKIKAGNLGAEFWSIW
                     VDPKTYKGQEIKRALDMIDSVYEQARRHPDKIMMAYSTQDILAAHRQHKLASLMGVEG
                     GHALQGDIRVLRDYYRLGVRYMTLTWSNTNELGDSSGDLDDKTVEHHKGLTDFGRQAV
                     REMNRMGMMVDISHVADRTFFQALTTSRAPVIASHSSSRAITNVPRNMTDDMLVALAR
                     NGGVAQVNFYCGFISQQWADQAKKLAADKDPDYEKVQSLVMSQHTPESMKQLYEAMAV
                     LEKKMPRPPLSDLIDHIDHMVKIAGVDHVGLGSDFDGIDCAPQGIDSAADLPKITEAL
                     YNRGYKADDLQKILGGDLMRVFAEVEKTAKQIQAEEAHQDTRREVAPDAQPKQ"
     gene            complement(10707..11129)
                     /locus_tag="VFC15_07890"
     CDS             complement(10707..11129)
                     /locus_tag="VFC15_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM10117.1"
                     /translation="MNARFFLDTNIFVYSFDRDSASNARRATQLIHQAVATRKGMVSY
                     QVVQEFFNVALRRFAQPMTVAEAEQYLGTVFRPLLAVQSSQALYSAALRLRDKHRLSW
                     YDALIVAAAMEGGCSLLYSEDLQSGRKFGDLRIENPFA"
     gene            complement(11119..11337)
                     /locus_tag="VFC15_07895"
     CDS             complement(11119..11337)
                     /locus_tag="VFC15_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10118.1"
                     /translation="MKNVTLSADEDLIEQARLVARSQRRTLNAAFREWLLQFTAQAGN
                     GQEVDALMQRLRHVSPGRRVSRDEMNER"
     gene            11545..11853
                     /locus_tag="VFC15_07900"
     CDS             11545..11853
                     /locus_tag="VFC15_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZM10119.1"
                     /translation="MNRLIEDKRRELELICRNRKVQQIELFGSATGPTFDPASSDLDF
                     LVTFDDLPVSSYADAYFGLLEDLQRLFQRNVDLVIESSIQNPYFRQSIDATRTLVYAA
                     "
     gene            11843..12184
                     /locus_tag="VFC15_07905"
     CDS             11843..12184
                     /locus_tag="VFC15_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HZM10120.1"
                     /translation="MRLEAKKYLHDITRSAGLALQFVAAKAYLDYVSDPLLRSAVERQ
                     MQTVGEALAQLAKIDPDTAARITEHQKIIAFRNILVHGYANIDDRVVWGLLELKLPQL
                     WREAEALLAEG"
     gene            complement(12265..13017)
                     /gene="sdhB"
                     /locus_tag="VFC15_07910"
     CDS             complement(12265..13017)
                     /gene="sdhB"
                     /locus_tag="VFC15_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZM10121.1"
                     /translation="MANSKTVIIKIKRQDTPTSNPYWEEFELPYRPGMNVTSSLMDIA
                     ANPVTKQGKATAPITYDSNCLEEICGSCAMLINGKARMACSALIDKLEQPISLEPFSK
                     FPVIRDLSVDRSVLFENLKKVKAWVPVDGTYDLGEGPRQSAEQQEEAYPLSRCISCCC
                     CMEACPQFNDHTGFVGAATISQVRLFNTHPTGKALKVERLKALMGDGGIQECGYAQNC
                     VEVCPKDIPLTKSIADVGGQVMWQAVKDLFRT"
     gene            complement(13004..13234)
                     /locus_tag="VFC15_07915"
     CDS             complement(13004..13234)
                     /locus_tag="VFC15_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10122.1"
                     /translation="MNLTGDIGRPQRVVYFLIGLALIACSWIAHDLLSWFQIVVLLLA
                     GIFLLWLAQAGRCEACEALPATGIQKNENGKF"
     gene            complement(13356..15143)
                     /gene="sdhA"
                     /locus_tag="VFC15_07920"
     CDS             complement(13356..15143)
                     /gene="sdhA"
                     /locus_tag="VFC15_07920"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZM10123.1"
                     /translation="MAHPKIIVVGGGLAGLSAAIKIAEMGGHVDLFSIVPVKRSHSVC
                     AQGGINAAKNLKGEGDSTWQHFDDTVYGGDFLACQTPVKAMCDQAPAIIDLLDRMGVP
                     FNRTPEGLLDFRRFGGTLYNRTAFAGATTGQQLLYALDEQVRRYEAEGKVTKYEGWEF
                     LSAVIDEQRVCRGICAMDLRGMEVVTFPAEAVIIATGGVGAIFGKSTNSVVCTGSAQS
                     ALYQQGAYYANGEFIQVHPTCIPGEDKLRLMSESARGEGGRVWVPRELGDKRDPTSIP
                     DSDRWYFLEEWYPKYGNLVPRDIATRAIHKVVYEHNLGIDGSPMVYLDLTHIDRATLD
                     RKLEGILEIYEKFVGDDPRDVPMKIFPGVHYTMGGLWVDFDQKTNIEGLYACGEADYS
                     IHGANRLGANSLLSCITGGFVAGPGAVQYARNVIKSGYTPGQHVFDSEKKRQQDINNR
                     LMNNDGTENPFLLWRELGDTMTKNATVIRYNKGLKETDAKLVELLERYRHVNLSDRTM
                     WANTSFVFARQLYNMLQLARVITQGAALRDESRGAHYKPDFPERDDANFLKTTKAYFA
                     ADADEPRFEYEAVDTSLIVPRPRRYDVAT"
     gene            complement(15201..15956)
                     /locus_tag="VFC15_07925"
     CDS             complement(15201..15956)
                     /locus_tag="VFC15_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10124.1"
                     /translation="MASSASPTVIDKGTVQIPQGVPSLRAGEGSSFVLRRLHSLTGII
                     PIGAFLIEHFISNSEATKGALAYNDQVKFLTGLPFVSVLEWGFIFLPLLYHSIYGFYI
                     WYRGESNVGEYPWTGNWLYTSQRWTGMIAFFYIAYHLYYMRFSGVHLMNGGYNSAFWK
                     VQHEFQNPWAIAAYVIGITAASWHFSYGLWLFCAKWGITVGDKARKRFGYVCLGLAVA
                     LLAIGLITIRAFVSAPLVPEPSPVTTLETTSLH"
     gene            complement(16178..16825)
                     /gene="rdgB"
                     /locus_tag="VFC15_07930"
     CDS             complement(16178..16825)
                     /gene="rdgB"
                     /locus_tag="VFC15_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZM10125.1"
                     /translation="MKRVLVATSNEGKIRDLVGAAAAHDIQIATLPDFASLPSVIEDG
                     LTFEANARKKAEEYSHFSDGELVIADDSGLEVDALQGAPGVRSARYAADEHNSLDDAT
                     NSNDEANNARLLRELHDVPAEYRTGRFVCVIAVARNGHTLATFHGKAEGVILHAPRGS
                     NGFGYDPLFFFPQIEKTFAELTAEEKAQYSHRGAAFRQFLEWADHLGDLHPHNAM"
     gene            complement(16859..17584)
                     /locus_tag="VFC15_07935"
     CDS             complement(16859..17584)
                     /locus_tag="VFC15_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10126.1"
                     /translation="MRVTEKAVAIIILTFSAWAIVMWLATSFQGNEPNRILRVELGED
                     AAQLNQAVAGPDNDAIAHNIEMVVRNTYMDFVFILLYWLTFVGLAMLAGRMGKRVLGA
                     CAALLITGAAVNDLLENGAILTAMRVKPFTDAVAVDISEYSQWKWVFFFVASILLGLA
                     IALNDRVSNVRRLSGGLFIASGVFGLLGIMRYRVSLEYSLWMIDIGILLIAAALILTL
                     WKIYHSIREIDRVEHMDRVHAHA"
     gene            complement(17787..18956)
                     /locus_tag="VFC15_07940"
     CDS             complement(17787..18956)
                     /locus_tag="VFC15_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM10127.1"
                     /translation="MPDDPTPSHALSPVHDQPGDPKQPTDGIALCLSGGGYRAMLFHL
                     GTLWRLNELHYLPKLNRVSSVSGGSITSGVLAMNWTKLGFDANGFAANFKQQVVQPIR
                     RMASDSIDVSSILEGIFGGVSKHVVAHYNEYLYNHAKLKDLPNDDEGPRFVFNATSLQ
                     TGVLWRFSKPFMGDWKVGLVRDPDIELAVAVAASSAFPPLLSPCVVPLDPDSFDKDIP
                     PAPDVTLPEFRQQAVLADGGVYDNLGLETAWKQNKTVLVSNGGGALGAEPDPKHDWVS
                     QTNRVLLTIYAQVVTLRTRSIIELYNLGVRDGSYWGIGSNILNYDLPDALVCPFDVTI
                     KLAQTATRLTAMDDPLQEQLIDWGYAICDAAMRKHVLPPGATAPESSPYGTFRKP"
BASE COUNT         4003 a         5352 c         5888 g         3953 t
ORIGIN      
        1 gaagaacacg gcatggtcgg acgccgctac gcaggcaagg tgcagcaaat tattacaggc
       61 tccatcggct tcgacgattg ggagtggggg gtggacctgt tcgctcccga tccgctggtg
      121 ttcaagaagc tgatttacga aatgcgcttt gaccacgtca gcgcagtgta tgccgccttt
      181 ggacagttct tcgtgggact gcgggttcca gtggcgaagc tgagtgagtg gactagcggg
      241 gaattgccga aatagtaata agtaataagt aataagtaat aagcgttcgc caacactaga
      301 accgaagacc tgttgcgcgt cgctctacat ttttagatcc ggggctctct tcctaaatca
      361 tgatcgctca cattattgag attcttgccg gttggattat tggcgtcatc tctgcactcg
      421 gatacccagg cattgtgctt ttgatggcga tcgagtcagc gtgtattcca ctgccatcgg
      481 aagttatcat gccgttctcg ggcgcactca tcgttcccgc ggtggcgagt ctgtatcacc
      541 gcgacccatt ctcgttgttc tgggttgcaa ccttcggagc gattggatgc aacttgggct
      601 cggtgctggc gtacgagatc ggatacttcg ggggacgccc gctggtcgag cgattcggcc
      661 ggtatgtgtt cctgaataag cgggaactgg atattgccga tcgcttcttc gaacgccgcg
      721 gtgcgattgc ggtattgatt gcgcgcctgc tgcccgtcat tcggacgttc atcgccttgc
      781 cggcgggaat tgcgcgcatg ccgcggctga agttccatgt ctatacgttc cttggatcgt
      841 ggccgtggtg cttcgcgctg gcgtacgtcg gcatgcggct gggcgagcag tgggacaagg
      901 acccacgcat caagatgtgg tttcaccgca tggatggcgt gatcctcgcg gtgttgattg
      961 ccgggatcgc gtatttcgtg tggtcgcacg tcagagaccg ggtgcgggaa gccgcctagt
     1021 ttgtttctgt gccgtcccaa cgggactcga atcatacccg ggctaaagcc cgtcgaaatt
     1081 gagatacccg cttcaccacg ctaaagcgtg gtgcttccac ccaatagctc gaggtttggc
     1141 gatattcatt tcactggtcg attttgacgg ctttggcgat catgcgctcg tagctcttcc
     1201 agggcagaat acgtctcagc cagacttgga tcttggcgtc tgtgccgatg aggtaacgga
     1261 gcttgggatt gggatcgttg gcgatgcgca agatgagttg ggcgatctct cgcgcgtcgc
     1321 gacggttttt ggcgctttgc ttgacgaact cggtgaagcg ctgactgcgt tctttgttgg
     1381 gtgagttggg atcgatcgct ccctggccga tcacgacatt gcgggtccag atgtcggtat
     1441 cgaaggcgcc gggttcgacc agcacaacgc ggatgccgag cgagtgtgtt tcaatgcgca
     1501 gtgattcgct gaacccctcg agagcgaact tggaactgct ataggcgccc agcaggggct
     1561 ggcccaggcg tccagctacg gacgcgacct gaacgatgtg gcccgaattc tgttggcgca
     1621 tcaccggcag cacagccttg gtcatggcga cattgccgaa gaagttggtt tcaaactgaa
     1681 gacgtatttc gccgagcgtc aggtcttcgc caaatccagc gacgctgaag ccggcattgt
     1741 tcaccagcac atcgatgcgg ccgtgagcgc gcacaatgtc gtcgattgct ccgggaaggg
     1801 aatcgaactc agtaacatcg aggcggcgga gatcgagccg gtcgcggact ccggcctttt
     1861 gcgcagtctc ctccagccgg ccggcgcggc ccagatcgcg catggtggcg acaacctggt
     1921 agccgtttcg ggcgaattca atcgtcgtca gcaacccgat gccgctggag cttcccgtaa
     1981 ccactgcgat cttctgaata ctcatttttc tccaccaaat ccttcatatc gccaacgaag
     2041 cgcaaaatgg gtccattcgc gtcgctaagt ttgcaaagta cactctggat acgagaagat
     2101 aacaggtttg tagatgcttc aagaaacgga ctgatacgta tgccaactgg aaagacgcag
     2161 gacaagagca agactgttaa gggcgcagtt gcgccagcca agggcaaact cggaatcatg
     2221 attcccggca tgggcgcggt tgccaccacg tttgtcgcgg gtgtggaagc cgtgcgcaaa
     2281 gggattgcaa gccccatcgg gtcgctgacc cagatgggca ccattcgact cggcaagcgt
     2341 accgacggcc gctcccccaa agtaaaagac ttcgtgccgc tggctggcct gaacgacctg
     2401 gtcttcaccg gctgggacat cttcgaggac gacatgtacg cggcggcggg taaggcggga
     2461 gtactggaac gttcgctgct ggagcaggta aaaccgttcc tgtcgtcgat caagccgcgc
     2521 aaggctgtgt tcgacaagaa ctacgtcaag aaactcgacg gccccaatgt caaaaagggc
     2581 aagaacaaga aggaactgtg cgagcagttg cgcgaggaca tccgcgactt caaacagtcc
     2641 tcgaaaaccg aacggcaggt gatgatctgg tgcggatcga ccgagagctt tattcaagct
     2701 ggtccggtgc atcagacggt caagtcgttc gaaaaggggc tgctcgccaa cgacgagaac
     2761 attccgcctt cgatgattta cgcttacgcc gccatgcagg agggcgttcc tttcgccaac
     2821 ggcgcgccca atctcacggt cgatgtgcca gtgatccagc aactgtcgag acagaacgaa
     2881 gcgcccatct gcggcaagga ttacaagacg ggacagacgc tgcttaagac cattctcgcg
     2941 cccgggttca aggcgcgcat gctgggactt agcggatggt tttccaccaa catcctgggc
     3001 aaccgggatg gcgaagtgct ggaagatccg ggctccttca agactaagga agagtcaaaa
     3061 ctgtcagtgc tggagcacat tctgcagccc gagttatacc ccgaactcta cggccatatt
     3121 tatcacaagg tgcgcattaa ctattaccct ccacgtggtg ataacaaaga aggttgggac
     3181 aacatcgata tcttcggatg gctgggctat ccgatgcaga tcaaggttga tttcctgtgc
     3241 cgcgactcga ttctcgcggc tcctctcgtg cttgacctgg tgctgctgct cgacctggcg
     3301 aaacgttcgt ccgagttgcg cggcatcggc atccaggagt ggctgagctt ctacttcaag
     3361 tctccgatga ctgcgccggg actttacccg gagcatgatc ttttcatcca gtcgatgaag
     3421 ctgaaaaaca ccatgcgcca cctgatgggc gaagagatga tcacgcacct ggggctggag
     3481 tattacgact aagtcgacgt ggtcagttta acccgaggag aacgacaaaa gacccactca
     3541 agcaaaaccg gcttgcgtgg ggcacccggc acccggcatc cgatgttcgg atgagggtag
     3601 aatagggcga tgagtccgga aatatctgag ttgctaaaac gggcgcttgc ccttccggcg
     3661 gacgaaagag ccgcattggc caatagcctt ttggacagcc ttgagattac gaatgaatcc
     3721 gtccaagagg catgggacga cgaagttgcg cgcagaatcg atgatctgaa ggctggcaga
     3781 gcagtgaccg tcccttggga agaactgcat cgtgagctac tggctatggt gaatggccgc
     3841 cagacgcgtt gagtaccacg agggcgctgt cgccgatgtg aagagcgcag ttttgtggta
     3901 ccaagagcgc agtcccaagg ctgcgatgga cttcatcggc gaattgcatc gagccgttga
     3961 caccatccgc gaggcaccga accgctggcc aatcggaagg aacaatacca ggcgattcct
     4021 gctcttgcgg ttccccttct tggttatcta ctcagaagtt gagtccgtaa tcacgatctg
     4081 ggccgttgcc cacggcaaca ggcgaccgga gtattgggca cgccgccttt agacgctgaa
     4141 gaacaccatg cgccaccaaa accggcttga gtggcgcacc cgcaagatgt ggaatgcccg
     4201 ggttcagacc ccagttggct gcaacgccgt ggtggcagat ttcgtcctcg acttttcgaa
     4261 taatttgatg cagtccagaa tcgtctccac tgccgtttcc tcgcccacct tggagttgat
     4321 catcaggtca aagcggtgcc ggcccggcca ttcgatgccg aaatattgcc tgataaaatc
     4381 ggcccggtcg cggtccacag tttcggcaag ctgaacggct tcactctcgc tctttccgcc
     4441 ggccttcagc cgccggacct tctcactcaa gggcgcgtaa acaaatacgt gaaaggcgtc
     4501 gcgacggttt tggagatagt gggccgaccc acgtcccaca atgacggact gaccttcttc
     4561 ggcggcgcgt ttcacgacac gctcggcgac ctcccgtacg caatcggtat cggccatttt
     4621 cagccgcggc gcgttcaagc ttccctcgaa gctgccgcgc atgaaggcca tcaggagccg
     4681 gtggtacaac gggtcccgtt tctcttcgtg ttgctccacc acgcggcact cgcactccat
     4741 caggcgggcg atctcgttgg ttaagagctg gtcccacaac ttccagccca gacgatcggc
     4801 gattcgtttg gcgatctctg cgccgccgct tccgtattcg cgttcgatgg taatgactcg
     4861 gatcatggtt cgctctctgg aaattcgccc aggtgctgca agaacccctg cagtggctct
     4921 gggattcggg cccacgctta atgctaatcc accaccaccc tgcgagcgcc aggatcattc
     4981 ttccgcaatg cgaaggaaag tacaaacatg gtcgccgcca tcaccgccag gacgagatag
     5041 gtatcgacgt aggccagggt ggtcgcctgg acgatgactg tccggtaaat aatggcgtag
     5101 gcctgcctgg tggctccggc ggcatcgacg cccgaggcaa atatacgcgc cgttaaggcc
     5161 tcggcagcct gggaaaaaga aagctgtccc ggtgcgacat tcgccaccag atgcacttga
     5221 tgaaattgtg cgcggcgggc aatcaccgtg gtgaccatgg aggtgcccac gctgctgccg
     5281 atgttgcgca tgaagttgac caggcccgcg acgctgctgc tcttctcggt cggcattcca
     5341 acgtaggatg cgaggttgat gggaacgaac aggaaaccca ggccgaacac ctgcaccagg
     5401 cgaatcatgc tggcgctgcg gaagctgatt ccgagattga gctcacggct ggagtaatac
     5461 atcccaatgg acaacgcgaa ccagccaaac gcaatgagat agcgggcctg caccttcgac
     5521 gagagcaccc cgatcatcgg catcatgaac agcagtagaa ggccgccacc cgaaagcacc
     5581 agacccgccg actccgcggt gtaccccagg agagactgca ggaacagagg catcatgacg
     5641 aggctggcga acagcatcaa tcccaggatg aacatcatgc cgttggctgc caggaagttc
     5701 aggtttctga acaggcgcac ctcgatgacc gggttcttgt agaaccactc ccaaacgacc
     5761 agtgaaacca ggccgaccac ggccagcaca gccagcgtga gaatgaagtg cgatccgaac
     5821 caatcgtcct cctgcccctt atcgagcatg atttgcagcg cgccaactcc gaggacgagc
     5881 aacgacatgc caacatagtc gatcctggag gcggcgccgg ctttgcgctt cgcccacggc
     5941 gggtcgtcga tcaaacgata aaccaggaac agtgccagta tcccgatcgg caggttgatg
     6001 aagaagatcc agcgccaggt gtagttgtcg gtgatccaac cgcctagggt cggcccaatg
     6061 gtaggtgcga cgatgacggt gacgccgtag agtgcgaagg ccaggccgcg cttttcagga
     6121 gggaacgtgt cggccaggat tgcctgggcc atcggttgca gcccgcctcc gccggcacct
     6181 tgcaggatgc ggaacaggat gatcgctccc aggctcggcg cgaacccgca caagagggag
     6241 ctcacggtga agatggcgag gcacgtcatg aaaaagcgct tgcggccgag cacgctggcc
     6301 agccatccgc tgatgggcaa caccacggcg ttggagacga ggtacgaagt gagcacccat
     6361 gtgctctggt cggtgctcgc tcccaggttg cctccgatgt agggaagcgc aacgttggcg
     6421 atgctggtgt cgagcacctc catgaacgcg gcgacggcca ctaccacccc gatcagccag
     6481 ggattgaact tgggccgcca agtcgcggac gcttcggaaa ccggagcgtt ggcggttgta
     6541 gtggctccgg gcggtgggtg ttccagattg gccgcgctgt ctgtcatagg ttcgagttat
     6601 gagagcctag cctggaatcg caacatctat ttcgaagatt cgattcgcca cgcatccgaa
     6661 aagctcttaa aaaattaccg ggcgatttgc cgtgtcggta cctggaggcg gagtttcggg
     6721 gaacccggtt cgccaccgtg catcccttat aattcgattc cttatgtcta ctcgtaaccg
     6781 gttgtgcctt gtcgcgctgg cgatcgtcct catcgccggc cttggtcatt cgcagaattc
     6841 acccgtcatg ctggaagctc tgaagccgga ttcgccgctg caggaaaact tgcgcaagct
     6901 gaccgacgag atcggcgggc gggttcccgg tacgccagcg tttacggcgg cggtgctgtg
     6961 gggcattgcc ggattcaaag cggccggcgc cgactcggtg catgtcgaag agttcaccat
     7021 cgcgcagtcg tgggcggaag gcgcaacgca agtcgaggtg gtctcgccgg ttaagtggag
     7081 actgctggcg cactccattg catggggccc ggcgctgaaa ccgacgacgg ctcgggtggt
     7141 ggatgtgggc atgggcaagc ctgaggactt cgccaaggca ggcgacatct cgggagcgat
     7201 cgtgttggct cactccgtgg tgctgaagac ctgggacgac ctgttcgatg aatactttcg
     7261 cgcacccggc atcattcagc aggcagtgaa ggggaaagcg atcgcgattg cattcgaatc
     7321 cagccgcgag cacgacatcc tctatcggca tctcaactca cagacgggcc acatcgatgt
     7381 gattccacaa gtgctgttgg cgcgggaaga cgccgagcgg gtggcgaggt tgattgcgca
     7441 tggcgagaag attgagatgt cggtctcgat gccgaacgtc atcggaccga ccattgcgac
     7501 tagcaacgtg attgctgaca tcaagggaac ggagctaccc gatgaagtgg tgatgctggg
     7561 cgcgcatctg gattcgtggg aactcggcac cggcgcgctg gacaacggct gcaacgcggc
     7621 gctggtgatt gacaccttgc gggcgatcaa agcggcgggt gtgcgaccgc gcagaacgat
     7681 tcggtttgtg ctttactccg gcgaagagca ggggctgctg ggctcgtggg cccatgtgcg
     7741 cgctcatcag aaggacctcg acaacatcgt ggccgaactg gtgctcgacg aaggcagtgg
     7801 cccgatcacg ggattttcga caggcggacg gaaggacatc gatgccgcgg tgaaacccat
     7861 ggtccaaccg tttgcgcagt ggaaagctgc cgagctgacc aacgacgcca tgaccggcac
     7921 ggatcattac gacttcatga tcgaaggcgt tcccacgctg gtggccaacc aggcggaagc
     7981 gaattacctg gtgaattacc atgcgacgtc cgacacgtat gacaaggttg atttcgcgca
     8041 gttgaagaag aacgaagcga tggcggcgga gttaatggtg cagatggcga acgcgccaca
     8101 gcgcatcggg ccgcgttgga cgcgggcgca aatcgaagcc agcttcgccg agactcatct
     8161 cgatgaccag atgaagggct tcggcatgtg ggacgactgg caaagcggaa cgcggggacg
     8221 gaagcagtaa cccgcgcttc accggggttg acagtggagc aagaaaaagc cgggcgcgtg
     8281 gcccggcatt ttctatccca gtcgaaagct attggggagg tggcgccgga gtcgacttct
     8341 taggagcggc agtgccggtc gtgctcttgt gtgcatgcgt gatggtcgtc ttcttcgccg
     8401 gagcagccgc cggatgacgc gggtcgatga tcttcacgtg cgtgatcttc ggcggattga
     8461 atggccggtc gttgcgtgga tcggtagcga ggcgggtgat ctgcttcacc agttccgggt
     8521 tgtcgcactg gccgaagatg gtgtgcttgc cgttcaagtg cggcgtcggg acctcggtga
     8581 tgaaaaactg ggagccgttg gtgtgggggc cgctgttggc catcgcgaga cgtcccggct
     8641 ggtcgaaggt gaggtcggtg aattcgtcct tgaagtcgta accagggccg ccgctgccgt
     8701 tacccagcgg atcgccgccc tggatcatga agttgggaat cacgcgatga aaaatcgtgc
     8761 catcgtacag cggcttgttg tgcatggtaa cgccgctggc cgggttcttc cagtctttct
     8821 tgccggttgc gagcccgatg aagttgtcca cggtgaccgg cgccttgtcg ggaaacagcg
     8881 tgcaggtgat gttgcccatg gtggtttcga agacagcctg caccggtgcg gtgctggtcg
     8941 cggctgcagg agtggccggt ttggttgcgg ctggcttggc cgctgtggat ttcggcgtgg
     9001 cgctgctgct ggattgcgcg atggcgaatc ctgcaagacc caggcaaagt attgcggtca
     9061 caaagttacg catggttcct cccgaaaaga cctacctcag cggctaaagc cgcgtccttg
     9121 gttatcaatt taatgcaccg ctaaagcggc gctccaccca ctacagctaa ttcaaaaggc
     9181 atttactgct tcggttgcgc gtcaggagcg acctcgcggc gcgtgtcctg atgggcttct
     9241 tccgcttgaa tttgtttggc agtcttctcc acctcggcga agacgcgcat caggtcgccg
     9301 ccgagaatct tctgcaaatc gtcagccttg tagccgcggt tgtacaacgc ctcggtgatt
     9361 ttcggcaggt cggcggccga gtcgatgccc tggggcgcgc agtcgatgcc atcgaagtcg
     9421 gagccgagtc ccacgtgatc gacaccagca atcttcacca tatgatcaat gtggtcgatc
     9481 aggtcgctca gcggcggacg cggcattttt ttctccagca ccgccatggc ttcgtataac
     9541 tgcttcatcg attccggcgt gtgttggctc atgaccagcg actgcacttt ttcgtagtcg
     9601 ggatctttat cggccgccag cttcttcgcc tggtcggccc attgttgcga gatgaatccg
     9661 caataaaaat ttacttgcgc aacgccaccg ttgcgggcca gtgctaccag catgtcgtca
     9721 gtcatattgc gcggcacgtt ggtgatggcg cgcgacgagg agtgcgaggc gatcaccgga
     9781 gcgcggctgg tggtcaaagc ctggaagaat gtgcggtcgg ccacgtggga gatgtcaacc
     9841 atcatgccca tgcgattcat ctcgcggacc gcctggcgtc cgaaatcggt gaggcccttg
     9901 tggtgttcga cggtcttgtc gtcgaggtcg ccgctggagt ctcccaactc gttggtgttc
     9961 gaccaggtga gcgtcatgta gcgcacgccc agccggtagt aatcgcgcaa cacgcgaatg
    10021 tcaccttgaa gagcgtgtcc gccttcgacg cccatcagcg acgcgagttt gtgctgacga
    10081 tgcgcggcca ggatgtcctg ggtgctgtag gccatcatga ttttgtcggg atggcggcgc
    10141 gcctgctcat acactgaatc gatcatgtcg agcgcccgct tgatctcctg tcccttgtac
    10201 gtcttgggat caacccagat cgaccagaac tccgcaccca ggttgccggc cttgattttg
    10261 ttgaagtcca tgtagccctt gccggcattc tgcgcgagat cgaagttctc gtccacgaag
    10321 cggcctggcg tgtcggcgtg ggtgtcgatg atgagggcgg agttgtggat ggcctgccag
    10381 cccgtggcca gtccattagg cgagatcgtg atggcgtggg aatgcgactt ggagggattc
    10441 ggcgttgtgg gagtggcagt ttgagcggat gaaaaagttg agagcagtag gatcgacgca
    10501 accacccgga tcatgggcat gaagttagag gactccgatg agattgtctc gcgatgattg
    10561 taaatgatgg ggaacagcca gactgatcga cgtgcacccg tggcagcgcc gaaaagcgta
    10621 gcgcgatacg gaacatcgga caccaggttt aagcgcactg taaagagggc cccaccattt
    10681 acggatgctc tcattcggca gcggcattag gcgaaggggt tctcgatgcg cagatcgcca
    10741 aacttgcgtc cggattgcag gtcttcgctg tagagtaggc tgcagccgcc ttccatcgcg
    10801 gcggcaacga tcaaagcgtc ataccaggac aatcggtgct tgtctcgcaa gcgcagggct
    10861 gcggaataca gggcctgcga agactgcacc gccaggagcg gccggaacac agtccccagg
    10921 tactgctcgg cctcggcgac ggtcatgggc tgggcaaatc gccgcagggc aacgttgaag
    10981 aactcctgca ccacctggta gctgaccatg ccctttcgcg tggccacggc ctggtgaatg
    11041 agctgcgtgg cgcgccgggc gttgctggcg gaatcgcggt cgaaactgta aacgaagatg
    11101 ttggtgtcga ggaagaacct agcgttcatt catttcatcc cggctgacac ggcggccagg
    11161 ggacacatgg cgcagccgtt gcatgagggc atcgacctcc tgcccattac cggcctgagc
    11221 ggtgaattgc agcagccatt cgcgaaaagc cgcgttcagc gtacgccgct gggagcgagc
    11281 gactagccgg gcctgctcga tgaggtcttc atcagcactg agggtaacgt ttttcatgcg
    11341 aatccagtgt acacgaaaat cgtgcgattt cggggtcaaa aactcccgac aaagcgtgcc
    11401 aacagatggg tgcccaatcg ccaagagagt cagtcgtgta cgggggctgc aacgctcatc
    11461 agcgaggcca gtttgtacga tctctgcgcg gccaggatcc aatgggtgct tgagttacta
    11521 tcgagtagat aggagaatgg ctcaatgaac cgcctgattg aggacaagcg tcgcgaactg
    11581 gaactcatct gccgcaaccg caaggtacag cagattgagt tgtttgggtc tgcaaccggg
    11641 ccaacatttg accctgccag cagcgacctc gacttcctgg ttacgtttga cgatcttcct
    11701 gtcagcagtt acgccgatgc gtactttggg ttgctggaag acctgcagcg attgtttcag
    11761 cgcaacgtgg acttggtaat cgagtcctca attcagaatc cctactttcg gcagtccatt
    11821 gatgcaaccc ggacccttgt ctatgcggct tgaggcgaag aagtacctgc atgacattac
    11881 gcgctctgcg ggacttgcac tgcaattcgt tgccgccaag gcctatttgg actatgtttc
    11941 agatccgttg ctgcggtctg cggtggagcg gcagatgcag accgtgggcg aagctcttgc
    12001 gcaattggcc aagatcgatc cggacacagc cgcgcgcatc acggaacacc agaagatcat
    12061 cgcttttcgc aacattcttg ttcacggata cgcgaatatc gatgatcgcg ttgtctgggg
    12121 tttgctcgag ctcaaattgc cgcagctatg gcgggaggca gaggccctgc ttgccgaagg
    12181 ctgacagtgc ggtcgaggcc cagaaacgaa aagccccgct ctcgccgcta tcgggcagaa
    12241 gcggggcaac gtcgttattg actcctacgt cctgaacaaa tccttcaccg cctgccacat
    12301 gacctggccg ccgacgtcgg cgatggactt ggtgagcgga atatctttag ggcagacctc
    12361 gacgcaattc tgcgcgtagc cacattcctg gataccgcca tcgcccatca acgctttcag
    12421 gcgctcgacc tttaaggcct tgccggtggg gtgagtgttg aacaagcgca cttgcgaaat
    12481 ggtggccgca cccacgaagc cggtgtggtc gttgaactga ggacaagcct ccatgcagca
    12541 gcagcatgag atgcagcgcg acagaggata ggcctcttcc tgctgctcgg cggactgacg
    12601 ggggccctcg ccaaggtcgt aggttccgtc caccggtacc caggccttca ccttcttcag
    12661 gttttcgaag agcacactgc ggtccacgga caaatcgcgg atgaccggga atttcgagaa
    12721 gggctcgagc gagatcggct gctccagttt gtcaatcagc gcggaacagg ccatgcgggc
    12781 cttgccgttg atcagcatgg cgcaagagcc gcagatttct tccaggcaat tggagtcgta
    12841 ggttatcggc gcggtcgcct tgccttgttt cgtaaccgga ttcgctgcga tatccatcag
    12901 cgacgaggtg acgttcatgc cggggcggta gggcaactcg aactcttccc agtagggatt
    12961 ggaggtcggg gtatcctggc gcttgatctt gatgattacg gttttagaat ttgccatttt
    13021 cgtttttctg tatgccggtc gccgggagcg cctcacaggc ttcgcagcgg ccggcttgag
    13081 caagccaaag taaaaatatt cctgctaaca acagcaccac aatctgaaac cacgaaagca
    13141 aatcgtgggc aatccacgag caggcaatca aggcgagacc gataagaaag taaacaaccc
    13201 gttgcggtct tccgatgtcg cctgtcagat tcatgcagcc ttcgtcaaat tcttccgggg
    13261 ctaaagcccc tcactatttc tagccgcctg gacgctggcc taaaggccag cactacccaa
    13321 aactagccac cagccactga ccactagcca ctgctttacg ttgccacgtc ataacgccgc
    13381 ggacgcggca caattagcga cgtgtcaacc gcctcgtatt caaagcgggg ttcgtcggca
    13441 tcggccgcga agtacgcctt ggtcgtcttt aggaaattgg cgtcgtcgcg ttcggggaaa
    13501 tccggtttgt aatgcgcgcc tcgcgactcg tcgcgcagag ccgcgccctg ggtgatcacg
    13561 cgcgccagtt gcagcatgtt ataaagctgc cgggcaaaca cgaatgatgt attcgcccac
    13621 atagtgcggt cgctgaggtt gacgtgacgg tagcgctcca gcaactcgac cagtttggcg
    13681 tccgtttcct tcaagccctt gttgtagcgg atgaccgtgg cgttcttggt catggtgtcg
    13741 cccaactcgc gccacagcag gaacggattt tccgtaccgt cgttgttcat cagacggtta
    13801 ttgatgtcct gttgccgttt cttttccgag tcaaacacgt gctggccggg ggtgtacccg
    13861 ctcttgatca cgttgcgggc gtattgcacc gcgcccggac cggcgacgaa tccaccggta
    13921 atgcacgaca acaacgagtt cgcacccaac cggtttgcgc cgtgaatcga gtagtcagct
    13981 tcgccgcagg catataagcc ttcgatgttg gttttctggt cgaagtcaac ccacagcccg
    14041 cccatggtgt agtgcacgcc gggaaagatc ttcatcggca cgtcgcgcgg atcgtcgccc
    14101 acgaactttt cgtagatttc gaggatgcct tccagcttgc gatccagcgt ggcgcggtcg
    14161 atatgggtga ggtcgaggta aaccatcggc gagccgtcga tcccgaggtt gtgctcgtac
    14221 acgactttat ggatggcacg ggtcgcgatg tcacgcggca ccaggttgcc gtacttggga
    14281 taccactctt ccaggaagta ccagcgatcg ctgtcgggaa tgctcgtggg atcgcgcttg
    14341 tcgcccaact cccgcgggac ccagacccgt ccgccttcgc cgcgtgccga ttccgacatc
    14401 aggcgcaatt tgtcttcgcc cggaatgcag gtgggatgca cctggatgaa ttctccgttg
    14461 gcgtaataag ccccctgctg gtagagcgcc gactgcgccg atccggtaca gacgaccgag
    14521 ttggtggact tgccgaagat cgctccaacg ccgccggtgg cgatgatgac agcttccgcc
    14581 gggaaggtga ccacttccat gccgcgcagg tccatggcgc aaattccgcg gcagacacgc
    14641 tgctcgtcga tcaccgcaga gaggaattcc cagccttcgt acttggtgac cttgccttcc
    14701 gcctcgtagc ggcgtacctg ctcgtcgagc gcgtacaaca attgctgccc agtggtggcg
    14761 ccggcgaagg cggtgcggtt gtacagcgtt ccgccgaagc ggcggaagtc gagcaggcct
    14821 tcgggcgtgc ggttgaaggg gacgcccatg cggtcgagca gatcgatgat cgccggggcc
    14881 tggtcgcaca ttgccttgac cggcgtctgg cacgccagaa agtcgccgcc gtacacggtg
    14941 tcgtcaaagt gctgccaggt ggagtcaccc tcgcccttca ggttcttggc ggcgttgatg
    15001 ccgccctggg cgcagaccga gtgagaccgt ttgaccggga cgatggagaa gaggtcaacg
    15061 tgacctccca tctcggcaat cttgatggcg gctgaaagtc ctgccaaccc gccacccaca
    15121 acgatgatct taggatgagc catgattatc tttatgcagc ttaaataagg cggacgatcc
    15181 gcccggatta atgcgtcgat ctaatgcaac gacgtagttt caagtgtcgt aactggactt
    15241 ggctccggaa ccaggggagc gcttacgaaa gcgcggatcg tgatcaaacc gatcgctagc
    15301 agcgcaaccg caagcccgag acacacatag ccgaaacgct tgcgcgcttt gtctcccacc
    15361 gtgatgcccc acttcgcaca aaacagccag aggccatagg agaagtgcca cgatgccgcg
    15421 gtgatgccga tgacgtaggc cgcgatcgcc cacggattct ggaattcatg ttgcaccttc
    15481 cagaacgcgg agttgtaacc gccgttcatc aggtgcacgc cgctgaagcg catgtaataa
    15541 aggtggtaag cgatgtagaa gaaggcgatc atgccggtcc agcgctgcga ggtgtagagc
    15601 cagttgccgg tccagggata ttcgccaacg ttggactcgc cgcgatacca gatgtagaac
    15661 ccgtagatcg agtgatagag caggggaaga aagatgaatc cccactccag taccgagacg
    15721 aagggcaggc cggtcagaaa cttgacctga tcgttatagg ccagcgcgcc cttggtcgcc
    15781 tccgagttgg aaatgaagtg ctcgatcagg aatgcgccaa tgggaataat gccggtgagc
    15841 gagtgcagcc tgcgcagcac gaacgagctg ccttcgccgg cacggagcga gggaactccc
    15901 tgaggaatct ggacggtacc tttatcaata acagtaggac tggcggatga agccacgacg
    15961 ccgactcctt atgattcgcg gagtgatgac gcgaaccttg cattatcaat ttcgccaact
    16021 gcgcaagtca agaagctcgc cgacttgctt tgactgcgaa gcaatgggtg cacacaagcc
    16081 tctttcgtcc ctgcgggact gcataatcct gagcaacaca atttcgaacg ctcgttcagg
    16141 atagcggaga gggctggcga gtacgtggtg acgggcgtca catagcgttg tgaggatgga
    16201 ggtcgccgag atggtccgcc cactccagaa actggcggaa agcggcgccg cgatggctgt
    16261 attgggcttt ttcttcggcg gtgagctcgg cgaaggtctt ctcgatttgg ggaaagaaga
    16321 acagcgggtc gtagccgaag ccgttggatc cgcgcggggc gtgcaggatg acgccttcgg
    16381 ctttgccatg gaaggtcgcc agcgtgtgtc cattgcgcgc cacggcgatc acgcagacaa
    16441 agcggccggt gcggtattcg gcggggacat cgtgcaactc gcgcagcagc cgcgcgttgt
    16501 tggcctcgtc gtttgagttg gtagcgtcgt cgagcgagtt gtgctcgtcc gcggcgtagc
    16561 gtgctgaacg cacgcctggt gcgccttgca aggcgtcgac ctccagcccg gagtcgtcag
    16621 caatcaccaa ttcaccatcg ctgaagtggc tgtattcctc ggccttttta cgggcgttgg
    16681 cttcgaaggt tagtccgtct tcgatcacac tggggagcga cgcaaagtcg ggcagggtcg
    16741 cgatctgaat gtcgtgggcg gcagccgctc ccaccaggtc gcggattttg ccttcattgg
    16801 atgtggcgac gagcacgcgt ttcatgactt tagaagttct gcttgacgca tcgccgattt
    16861 acgcgtgcgc gtgcacacga tccatgtgct cgacgcgatc aatctccctg atcgagtggt
    16921 agatcttcca caaagtgagt atcaacgcgg cggcaatcag cagtatgccg atgtcgatca
    16981 tccacaggga gtattcgagc gagacccggt agcgcatgat gccaagcagg ccgaacacgc
    17041 cactggctat gaacagtccg cccgacagac ggcgaacgtt ggacacgcgg tcgttcaagg
    17101 cgatggccaa tcccaggaga atcgaggcca cgaaaaagaa gacccacttc cactgggagt
    17161 actccgaaat atcgacggca acggcgtcgg tgaacggctt cactcgcatc gcggtgagga
    17221 tcgcaccatt ctccagcaag tcgttgacgg cagcgccagt gatcagcagc gcagcgcagg
    17281 cgccgagcac gcgcttgccc atgcgtcccg ccagcatggc gaggcccacg aacgtcagcc
    17341 agtagagaag gatgaagaca aagtccatgt aggtgttgcg caccaccatc tcaatattgt
    17401 gcgcaattgc gtcgttgtca gggccggcca cggcttggtt gagttgggcg gcatcttccc
    17461 caagttccac ccgcagaatg cgattcggct cgtttccctg gaagctggtc gccagccaca
    17521 tgacgattgc ccatgccgaa aacgtgagaa tgatgatggc gacagctttt tcagtcacgc
    17581 gcattgctat gtctccagac gagggcgttg gtggatttga ccggggagaa atggccacat
    17641 tccgttgtag agtgggcgat cccctccgcg gccagatggt tgtaatctaa cacggccaac
    17701 atttgaatga agcagtgtcc caagagctgg ggcaccttgt tcgtaacggt gcccacaatc
    17761 gcccgacaac cttcttgatg gtccgctcag ggtttcctga acgtgccata gggtgacgac
    17821 tctggagcgg ttgcaccagg cggcagcaca tgtttccgca tggcggcgtc gcagattgca
    17881 tagccccagt cgatcaactg ctcctgcaat ggatcgtcca tcgcagtgag ccgggtcgcg
    17941 gtctgggcca gcttgatggt gacatcgaac gggcagacca acgcgtccgg caaatcatag
    18001 ttcaggatat tcgacccaat gccccagtag gagccgtcgc gaaccccgag attgtagagt
    18061 tcaatgatcg agcgcgtgcg cagcgttacg acctgggcat agatcgtgag caacacgcga
    18121 ttcgtctggc tgacccaatc gtgtttggga tcgggttcgg cacccaaggc cccgccgcca
    18181 ttactgacca gaacggtctt gttctgcttc cacgcggttt ccaggccgag attgtcatac
    18241 acacctccat cggcgaggac cgcctgctgg cggaattcgg gtagcgtgac atcgggagcc
    18301 ggcggaatat ccttatcgaa gctgtcgggg tccagcggca ctacgcaagg cgagagcaga
    18361 ggcgggaagg ccgatgacgc cgcgaccgcg acggcgagct cgatgtcagg gtcccttacc
    18421 aggccaactt tccagtcgcc catgaagggc ttggagaaac gccacagcac tccggtctgc
    18481 aggctggtgg cattaaacac aaagcgcggg ccctcgtcat cgttcggtaa atccttcagc
    18541 ttggcgtggt tgtacagata ttcgttgtag tgggcgacga cgtgcttcga tacgccgccg
    18601 aaaatgccct ccaggatcga actgacatcg atcgaatccg aggccatcct gcggataggt
    18661 tgtacgacct gctgcttgaa gttggctgca aagccgttgg cgtcgaagcc cagcttggtc
    18721 caattcatcg ccagaactcc actggtaatg gacccgccgg agacgctgga aacgcggtta
    18781 agcttaggta gatagtgcaa ttcgttgagc cgccacagcg tgcccagatg aaacaacatg
    18841 gcgcgatagc cgccgccgga aagacacagg gcaatgccgt cggtcggctg cttcggatcg
    18901 ccgggttggt cgtgaacggg agacagagca tgggatggtg tgggatcgtc gggcataagg
    18961 cctccggtga actaaaactg aatgagactg gtttggagct gcaacgctga caggcgcact
    19021 ttagcactgt tttggccttg cggaaagcac gaacgtgagc tgtgtcggta gcacatgata
    19081 tgtccggttg agatagcacg tgatctgtcc gctttgcggg cggcgagagt aggagaccat
    19141 gcatagcatg gtggtttact cgaaggccgc gatggagcgg gcgatgaaag tgcagg
//
LOCUS       DASRKK010000360         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_907247, whole genome shotgun sequence.
ACCESSION   DASRKK010000360 DASRKK010000000
VERSION     DASRKK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907247"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFC15_07945"
     CDS             complement(<1..374)
                     /locus_tag="VFC15_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10128.1"
                     /translation="MAIGETTYMNSVREQLLAGRTRLADVLADNESEQMLRLLEEVDS
                     ALHRIDHGAFGVCEVCQGTVPAGDLLENPLLRVCLDCLTPKQQRALEYDLELAAQIQK
                     GLLPPTDLAIAGWEISYHYQPAG"
     gene            complement(705..1058)
                     /locus_tag="VFC15_07950"
     CDS             complement(705..1058)
                     /locus_tag="VFC15_07950"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HZM10129.1"
                     /translation="MYKTLMLTLVLLISAVWLQAQDAGQMGGKKSGVTTIEGCLQSSG
                     GKYTLTKADGTTVTLSSHANEMIHHVGHQVQVTGMPAVKTIDTTQTGTESSVKEIPVF
                     RVQSIKHVADTCTTK"
     gene            complement(1211..1558)
                     /locus_tag="VFC15_07955"
     CDS             complement(1211..1558)
                     /locus_tag="VFC15_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10130.1"
                     /translation="MRKTLVLTLVLLVSAVWVQAQDAGKASGKTADLDTIEGCLQHSE
                     GQYTLIDSTNTIHHLTGGASKLSHHIGHQVEITGKPGVRTLDTTAQGTASTAVVQPVI
                     EVKSVKHVADTCK"
     gene            complement(1591..>4336)
                     /locus_tag="VFC15_07960"
     CDS             complement(1591..>4336)
                     /locus_tag="VFC15_07960"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZM10131.1"
                     /translation="GIEKRDYSRATSSQKCVRAGGKHNDLENVGFTNRHHTFFEMLGN
                     FSFGDYFKKDAIAFAWELVTSPEWFGIEKDKLFATIFKGEQGIARDDEAYEHWIATGV
                     APERIYELGMKDNFWMMGDTGPCGPCSEIHYDMGPAASDAGHADCQFGCECGRYVEIW
                     NLVFMQFDKQPDGTMRPLPKPSIDTGAGLERVTAVMQGVISNYDTDLFVPLIKRAAEL
                     TGAPLDQELKKEDEEERHHQAASLRVIADHARASTFLISDGVVPSNEGRGYVLRKIIR
                     RAIRHGRLLGQDKPFLYEMAFAVRDLMKDAYPELIETTERVSETIKGEETRFAHTLDI
                     GLQRLQELLVGAQAPNPSSQLATQGEERLKESIERAQEGWHFSGTTDDGIRAATRELG
                     DKEGTPEDVAQFRVLDRRIPGAEAFKLYDTFGLPRDFVEDACRDAGIQFDAAGFDAAM
                     EEQRKRAQASWKGGSKASASPAYQSLQPTVFEGYHKTLSTGCEVLAIIKPHNGTGVGA
                     QELKPGERGEIVLDHTPFYADAGGQVGDVGWLYGEDHNTIIAEVDGVTYPVQGVRAHR
                     VIAKQPIHVGDKVDAMVNDEVRRAAMRNHTGTHLLHAALREVLGKHVKQAGSLVDPAH
                     LRFDFSHFASLDDEELQDIEDLANREVLRNDRVEVIEDVPIDVAVNEYHAMALFGEKY
                     GDRVRVIRIGDFSTELCGGTHTSATGEIGLIKVLKEGSVSSGVRRLEAITGEGSLRHF
                     RKDHQLENVVSTLVRGGDDQSPADALKQELERREEELKKLRKELDQVRMKSASTSVQS
                     AAENVREVKGVKVLATRADNLERAQLRVLIDNLRNKLGSGVVVLGSVSDGKVALIVGV
                     TKDLTSRVQAGRIIAEVAKKVGGSGGGRPDMAEAGGKDPAALDSALAESYSVVERLLP
                     "
BASE COUNT          778 a         1387 c         1228 g          943 t
ORIGIN      
        1 cccgcaggct gatagtgata ggaaatttcc cagcccgcga tggcaagatc ggtgggcggc
       61 aacaatcctt tctggatctg cgcggccagt tccaggtcgt actccagcgc gcgctgctgc
      121 ttaggcgtga ggcaatccag gcagacccta agcagcggat tttccagcag atctcctgcc
      181 gggacggtac cctggcacac ttcgcatacc ccgaaggcgc cgtggtcaat gcgatgcagc
      241 gcgctgtcca cttcctccag caggcgcagc atctgttcgc tctcgttgtc ggcgagcacg
      301 tcggcaagcc gcgtccgtcc ggccaataat tgctcccgga cgctgttcat gtaagtcgtt
      361 tcaccaattg ccatggctaa ccaccttata ccgtttagag ggttacggac aacggcagga
      421 tgcagtcttt ttcgcctgaa agttacctat cttgcctgtc attccgagcg agcccgcgcc
      481 tcgcgggcga gccgaagtgc ctgccccggt cgaagccgag ggaatctgcg gttccgtcct
      541 ttagcccccg tcccgattcg aactctaggc ttgattcctg gaggtgtcat cccgcatctt
      601 gcggtcaaag cactgggagg acaaaaaaag gcgggcgcga cgagagcccg cgacgcaagc
      661 cttcgtcgcg ccctgtagct tgtctgttat ccggtgcaca cccgtcactt ggtcgtgcag
      721 gtgtcagcta cgtgctttat gctctgtact cgaaaaactg gtatctcttt tacgctggac
      781 tcagtgcccg tctgggtggt gtctatggtt ttcaccgcag gcataccggt aacctggact
      841 tggtgtccaa catggtggat catctcgttg gcatggctgg aaagcgtaac cgtcgtccca
      901 tcggctttgg tgagggtata cttcccgccg gagctctgca ggcagccttc aatcgtcgtc
      961 acgccggact tcttgccgcc catctggccg gcatcttgcg cttgcaacca aacggcagag
     1021 atcagtagca ctagagtaag catcaatgtc ttgtacacaa acagcctcct tatcggcttg
     1081 cgacttagtt gcggtttaag tttgactcga taggcgacgc cgcagcatcg cccattggtt
     1141 cgatcgtgga gctacatccg taccaataca agggacacga ctcgaaggcg atcagtcgat
     1201 cagttgcccg ctatttgcac gtgtccgcta catgcttcac gctctttact tcaataaccg
     1261 gttgcaccac tgcagtggat gcagtgccct gggccgtcgt gtctagggtc cgcaccccag
     1321 gcttaccggt gatttcgacc tggtggccga tatggtggct gagctttgag gcgccgcccg
     1381 taaggtgatg gatcgtgttt gtgctgtcga tgagcgtgta ttgaccctca gagtgctgca
     1441 ggcagccttc aatcgtgtcc aagtcggctg tcttgccact cgccttgccc gcatcttgtg
     1501 cttgcaccca aacggcagag accagtagca ccagggtaag caccaatgtc ttgcgcacga
     1561 aatctccttt gctgctgctt gcggcctatt ctacggcaag aggcgttcca ccacgctata
     1621 actttcggcc agggctgaat cgagtgcggc gggatctttg ccaccggcct cagccatgtc
     1681 aggtcgcccg ccgccggagc cgccgacctt cttcgcgact tccgcaatga ttctccccgc
     1741 ctgcacgcgc gatgtcaggt ccttggtgac gcccacgatg agcgcgacct tgccgtcgct
     1801 gaccgagccg agcacgacca cacccgagcc cagcttgtta cgcaggttgt cgatcagcac
     1861 ccgcagttgc gcgcgttcca gattgtcggc ccgcgtggcc agcaccttca cgcctttcac
     1921 ctcgcgcacg ttctccgccg ccgactgcac cgaggtggaa gccgacttca tgcgcacctg
     1981 gtcgagttct ttgcgcaact ttttcagctc ttcttcgcgg cgctccagtt cctgcttcag
     2041 agcgtcggca ggagattgat cgtcgccgcc acgcaccaac gtcgagacga cattttccaa
     2101 ctgatgatct ttgcggaagt ggcgcagcga gccttcgccg gtaatggcct caagccgccg
     2161 cacgccgctg gagacgctgc cttccttcag gaccttgatc agcccgatct cgccggttgc
     2221 agaggtgtgc gtgccgccgc aaagctcggt ggaaaaatcg ccaatgcgaa tgacgcgcac
     2281 ccgatcgcca tacttttcgc cgaagagcgc catcgcgtgg tactcgttga cggcaacgtc
     2341 gatgggcacg tcctcgatca cctcgacgcg atcattgcgc agcacttccc ggttggcgag
     2401 atcttcaata tcctgcaatt cttcgtcatc cagcgaagcg aagtgcgaga agtcgaagcg
     2461 caaatgcgcg ggatcgacca gcgaacccgc ctgcttcacg tgcttgccca gtacctcgcg
     2521 cagcgcggcg tgcagcagat gggtgccagt gtggttgcgc atggcggcgc gacggacttc
     2581 gtcgttcacc atcgcatcca ctttgtcgcc cacatggatt ggctgcttgg cgatgacgcg
     2641 gtgcgcgcgc acgccctgca ccggataggt gacgccgtca acttcggcga tgatggtatt
     2701 gtgatcttcg ccgtaaagcc agccaacatc gccgacctgc ccgcccgcat cggcgtaaaa
     2761 aggggtgtga tcgagcacga tctcgccacg ctcgccgggc ttcaactcct gcgcgcccac
     2821 gcccgtgcca ttgtgcggct tgatgatcgc cagcacctcg catccggtgg agagcgtctt
     2881 gtggtagccc tcgaaaaccg tcggctgcag cgattggtaa gcaggactcg cgctcgcctt
     2941 cgaaccgccc ttccaggaag cctgcgcccg tttgcgctgc tcttccatgg cggcgtcgaa
     3001 accagcagcg tcgaattgaa tgccagcatc tcggcaggcg tcttctacga agtcgcgcgg
     3061 tagaccaaat gtgtcataca gcttgaacgc ttctgcacct ggaatgcgac ggtccagcac
     3121 ccggaactgg gcgacgtctt ccggtgttcc ctctttgtca cccagctctc gcgtcgccgc
     3181 gcgaatcccg tcgtctgttg tgccgctgaa atgccaccct tcctgcgctc gctcgatgga
     3241 ttccttcagc cgttcctccc cttgcgtcgc gagttgactc gaagggtttg gagcctgagc
     3301 cccaacgagc agttcttgaa gccgctgtaa cccgatatcc agcgtgtgcg caaagcgcgt
     3361 ctcttcaccc ttaatcgtct ccgacacgcg ctccgtagtt tcgattagct cgggatacgc
     3421 gtccttcata agatcgcgca cggcaaacgc catctcgtac aggaacggct tgtcctggcc
     3481 caacaagcgc ccgtgccgaa tcgcgcgacg aatgatcttc cgcagcacgt agccgcggcc
     3541 ttcattcgac ggcaccacgc cgtcggagat gagaaacgtt gacgcgcgcg catgatccgc
     3601 gatgacgcgc agcgaggccg cctgatggtg ccgctcttcc tcgtcttctt tcttcaattc
     3661 ctggtccagc ggagcgccgg ttagttccgc cgcccgctta atcaggggca caaacaaatc
     3721 cgtgtcgtaa ttcgagatca cgccttgcat caccgccgtt acgcgctcca gcccggcgcc
     3781 ggtgtcaatc gacggcttgg gcagcggcct catcgtgccg tcgggctgct tgtcgaactg
     3841 catgaacacg aggttccaga tttcgacata gcggccgcac tcgcagccga actggcaatc
     3901 ggcgtgtccg gcgtcgctgg cggccgggcc catgtcgtaa tggatctcac tgcacgggcc
     3961 gcaggggccg gtgtcgccca tcatccagaa gttgtctttc atgccgagct cgtaaatgcg
     4021 ctcgggcgcg acgccggttg cgatccagtg ctcgtaggcc tcgtcgtcac gggcaatgcc
     4081 ctgctcgccc ttgaagatgg tggcgaacag cttgtccttc tcgatgccga accactccgg
     4141 cgaggtgacc agttcccacg caaaggcgat ggcgtccttc ttgaagtagt cgccaaagct
     4201 gaaattgccc agcatctcga agaaagtgtg gtgacgattg gtgaagccga cgttctccag
     4261 atcgttgtgc ttgccgccgg cgcgcacgca tttctgcgac gaggtggccc gcgagtaatc
     4321 gcgcttctca atgccg
//
LOCUS       DASRKK010000361         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1298005, whole genome shotgun sequence.
ACCESSION   DASRKK010000361 DASRKK010000000
VERSION     DASRKK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298005"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1032
                     /locus_tag="VFC15_07965"
     CDS             346..1032
                     /locus_tag="VFC15_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZM10132.1"
                     /translation="MITVSKEDYLKAILEAEGEGEQVISARLAQWLSVSPPAVTMALR
                     RLKRDGLVSVRSDGRVQLTAEGRKIARRLAVRHHLIERMLHEIFGMEWYKVHDEAERL
                     EHAVSSDFEAMLTRKLGRGGPCPHGNSSTPEPPRIKRKRGLLLLSESQPGGRYAVQSV
                     YERDRRLGEFLEKLGIRPGAEIAVHLRNYDDTLSLVTTAGKVSLGRSAAEKVWVAPAN
                     GDSSSHPKKN"
     gene            1198..2550
                     /locus_tag="VFC15_07970"
     CDS             1198..2550
                     /locus_tag="VFC15_07970"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZM10133.1"
                     /translation="MSLEGMHSTVAVPHHEAGFWQQWRAFVGPAILVSVGYMDPGNWG
                     TDLQGGAQFKYGLLWVVGLASLMAIFMQVISARLGVVTGKDLAQCCRDWYPKWTRWPN
                     WLMSEVAIGACDLAEVLGSAVALNLMFHIPLLWAVIITGLDVLLLLAMQHFGMRTIEA
                     IVVLLIVTIGVCYFIEIFVLPQTQPSFLEMGRALISPRLRQAGMLYVAVGMIGATVMP
                     HNLYLHSALVQSRKLQKDELSMRRAVRFNTIDSTVALTIAFFVNAAILVLAAMVFFGK
                     ESVTVTGGQLIKFRPDSDWIRVAYLTLAPLLGTAFASTLFAVALLASGQSSTITGTLA
                     GQVVMEGFMHWRIKPWVRRLITRTLAILPAVFIIGLRGDSSVTDLLILSQVVLALQLP
                     FAMFPLLQFTSSRKRMGNWRNGWFLLLAGWGSAILITAMDVYSLPESLKAAWQVIAGT
                     "
     gene            2578..2994
                     /locus_tag="VFC15_07975"
     CDS             2578..2994
                     /locus_tag="VFC15_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZM10134.1"
                     /translation="MYDTILVTLDGTPTDRAIIEHIKQLAKLAHSHLVLLHVADGWAA
                     RTYGPDAISAEIAEDTAYLEKVRAEFQAIGIPTEAELAYGEPVKEIIKWVQRKGCDLV
                     AMSTHGHRFLADLFLGTTATQVRHSVSVPVLLLKAK"
     gene            3347..>3745
                     /locus_tag="VFC15_07980"
     CDS             3347..>3745
                     /locus_tag="VFC15_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZM10135.1"
                     /translation="MNSFGSRSILRVGKREFEIYRLDALEKQGCAVGHLPYSLRILLE
                     NLLRREDGKAVKADDVRALASWTGKTVPVQEIAFMPSRVLLQDFTGVPAVVDLAAMRD
                     AMKALGGDPTLINPLQPAELVIDHSVQVDEY"
BASE COUNT          721 a         1099 c         1144 g          781 t
ORIGIN      
        1 agcttcttga cccgcggcga ctgccgcagc ttccatacga tcgcatgttc gcgaccaccg
       61 ctaccgagga ccaggatctt catgggacca agttccacac gacttttctg ctggcgagaa
      121 ttagacgcga agcgaaagaa aagagtaggg gtgcaatgcg cccgctgtca aacttgcagt
      181 gcagctgctt ttccatctgt catcctgagc gaggtcgccg cggcgaccga gtcgaaggac
      241 ccctataagc gcgggatcgc tctgtcgccg tcttgcccag caacaaatct gggattgcct
      301 tgcaatcctc tattaactgt cgttaactaa cacttaacaa gtcctatgat tacggtttcc
      361 aaagaagatt acctcaaggc catcctggaa gcggagggcg aaggcgagca ggtcatctca
      421 gcccgcctgg cgcaatggct ttcggtttcc ccgccggcgg tcaccatggc gttgcggcgg
      481 ctcaagcgcg acggcctggt ttccgtgcga agcgacgggc gagtgcaact cacggcagag
      541 ggacggaaga tcgcccggcg actggccgta cggcatcacc tgatcgaacg catgctacac
      601 gagatcttcg gcatggagtg gtacaaggtg catgacgaag cggagcgcct ggagcacgcc
      661 gtctccagcg acttcgaggc catgctcacg cgcaaattgg gacgcggcgg gccttgtccg
      721 catggcaatt cgtcgacccc tgagcctccg cgcatcaagc gtaagcgtgg cctgctgctg
      781 ctttccgaat cgcagcctgg gggccgttat gcggtgcaaa gcgtttatga gcgcgaccgc
      841 aggctgggag agttcctgga gaagcttggt attcgccccg gagcagagat tgcggtccat
      901 ctccggaact acgacgacac cctgagcctg gtcaccaccg cgggcaaggt gtcgctaggc
      961 cgcagcgccg cggaaaaagt gtgggttgca cctgccaatg gcgacagttc ctctcatcca
     1021 aaaaagaatt aaccatggtt taataaatcc tgtatacttc ggctaaggat gtggaattcg
     1081 tggcgacgcc gtgtcgtcgt ctcctttttt cggtgagctt tgaacacgaa accccaggcg
     1141 ccgcctagcc cgcagaacgc gcgaccggat gaactccggg ccgccgacct cggtcacgtg
     1201 tcgttggagg ggatgcacag taccgtcgcg gtgccgcacc atgaggccgg cttctggcag
     1261 cagtggcggg ccttcgtcgg gccggcgatt ctggtcagcg tcggctatat ggatcccggc
     1321 aattggggga cggacctgca aggcggcgct cagttcaagt acgggctttt gtgggtggtc
     1381 ggtctggcca gcctgatggc catctttatg caggtcatct ccgcacggct gggcgtggtg
     1441 acagggaaag acctcgcgca atgctgccgc gactggtatc ccaagtggac gcgctggccc
     1501 aattggctga tgagtgaagt cgccatcggc gcttgtgact tggcggaggt gctggggagc
     1561 gcggtcgccc tcaacctgat gttccacatc ccgctgcttt gggcggtcat catcaccggc
     1621 ttggacgtgc tcctgctgct ggccatgcag cactttggga tgcgcaccat cgaggccatt
     1681 gtcgtcttgc tgatagtcac catcggcgtg tgctacttca tcgaaatttt cgttctgccg
     1741 cagacccagc ccagtttcct ggaaatggga cgggcgctga tttcgccgcg ccttcgccaa
     1801 gcggggatgt tgtacgtcgc cgtcggcatg attggcgcga ccgtgatgcc ccacaacctg
     1861 tacttgcact cggccctggt gcagagccgc aagctgcaaa aggacgaact gtccatgcgg
     1921 cgcgcggtcc gcttcaacac catcgactca acggtggcgc tgaccatcgc tttcttcgtc
     1981 aacgcggcca tcctggtgtt ggcggcgatg gtgttttttg gcaaggaaag cgtgaccgta
     2041 accggcggcc agttgatcaa gttcaggccc gacagcgatt ggattcgagt cgcgtacttg
     2101 acgctggcgc cgttgttggg gacggctttt gcgagcacgt tgtttgcggt ggccctgctg
     2161 gctagcggcc agagcagcac catcacgggc acgctcgccg ggcaagtcgt gatggaaggc
     2221 ttcatgcatt ggcgaatcaa accgtgggtg cggcggctca tcacccgcac gctcgccatt
     2281 ctgcccgcag tcttcattat tggcctgcga ggcgacagca gcgttaccga cctgctgatc
     2341 ctcagtcaag tggtgctggc gttgcagctt ccgttcgcga tgtttccgct cctgcaattc
     2401 accagttccc gcaagcgaat ggggaattgg aggaacggtt ggtttctact gctggcgggt
     2461 tggggcagtg ctatcctgat caccgcgatg gacgtttaca gcttgcccga atcgctgaaa
     2521 gctgcgtggc aggtcatcgc cggcacgtaa ctcggcgacg agacaaacgg gacgaccatg
     2581 tacgacacca ttctcgtgac gttggacggt actccgaccg accgcgcgat catcgagcac
     2641 atcaaacagc tcgcgaagct ggcacacagc catttggtgt tgctgcatgt cgccgacgga
     2701 tgggcggcca gaacctatgg cccggacgcg atcagtgcgg aaatcgccga agatactgcg
     2761 tacttggaga aagttcgggc ggagtttcaa gccatcggca ttcctacaga agcggagctc
     2821 gcatacggcg agccggtcaa ggaaatcatc aaatgggtgc agcgcaaggg ctgcgatctg
     2881 gtggcgatga gcacccacgg acaccgtttc ctggccgacc tgtttctcgg caccaccgcc
     2941 actcaagtcc ggcacagcgt cagcgtgcct gtgctgcttc tcaaggcgaa gtgagggatt
     3001 tcgttcgagc gagaacgaat tcacgctgca ccaacccgca aggtcccggc ttatcctctg
     3061 cccactatcc actaacctct cccacggttt aagataatta gtttcgcttt ctagagcgtg
     3121 tttcacaaat cggtttgtat cagggatggc ttcagccgtg ccgcaggggt ctttataact
     3181 atgtgggctt tagcccctgt cctcagcggc taaagctgtt atcttcgacc gccccggcgg
     3241 cacgactaaa aagcctgccc tgagcgcccg acctgcgaca atttttgaaa cacgttctaa
     3301 gaagctgcac ccagtcatcg ctcctctaat aggaagggat tcatccatga acagtttcgg
     3361 cagccgttcc atcctccgcg tgggcaagcg ggagtttgaa atctaccgtc ttgacgcgct
     3421 ggaaaagcag ggctgcgcgg tcggccacct gccttactcg cttcgcatcc tgctggagaa
     3481 cctgctccgc cgcgaagacg gcaaagcagt gaaggctgac gacgtgcgcg cgctcgcgtc
     3541 gtggacgggc aagaccgtcc cggtgcagga gattgcgttc atgcctagcc gcgtgctttt
     3601 gcaggatttc accggtgtgc ccgcggtggt tgacctggcc gcgatgcgcg atgctatgaa
     3661 ggcgctgggc ggcgacccga cgctgattaa cccgttgcag ccggcggagt tggtgattga
     3721 ccactcggtg caggtggacg agtac
//
LOCUS       DASRKK010000362         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_907351, whole genome shotgun sequence.
ACCESSION   DASRKK010000362 DASRKK010000000
VERSION     DASRKK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907351"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFC15_07985"
     CDS             <1..1138
                     /locus_tag="VFC15_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM10136.1"
                     /translation="TVQQRALDLTNKTLSDNQKQVSAGTLAPLAVVQSQSAVATAKQN
                     LITAQVNLQLQQLLMKNAITRNMADPMLAVAPVIPTDTLSTTEEYEVRPVEDLIDEAL
                     KQRPEIASARLTLTNNEISRKSIRNQLLPTVDVYAFYGAAALAGPQNPLCVNPNGCPP
                     PGTIPTTGYGDAFGNLFNSSAPDKGVGININIPLRNRQIQADQVRSVLEYRQAQMSLQ
                     QTENTIRLQVRQAQFAMQQNYVGLQATIAARDYAAQSLDAEQKKLRMGASTSTLVLQS
                     SSNLTQAESNVLIAATNYEKSKVQLDLSTAETLAKLGIDMSDAETAQVKHSPKVPGTV
                     PANTNDLTQPKLTPIQVPGVTPALQPGTPPQTPDQAQPPTPPQR"
     gene            1464..>2548
                     /locus_tag="VFC15_07990"
     CDS             1464..>2548
                     /locus_tag="VFC15_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZM10137.1"
                     /translation="MSDSRKPDGKVLAELPVTDLFESGRHPSESEKQWAEKTLAPSLE
                     KGPEKPIGSPTGVNADEHGHARYTTISGLPIRRLYTPADLPADWKYEDHLNYPGQPPF
                     TRGIHASGYRGKMWTMRQFSGFASPEETNERYKYLLAHGGGGLSVAFDLPTLMGYDSD
                     HPFSEGEVGKCGVAIDSLEDMEILFDGIDLEKTTVSMTINSPASILWAMYLAVAEKQG
                     ADWKKISGTIQNDILKEYIAQKEYIYPPAQSMRLVIDTFEFGSIFTPRFNTISISGYH
                     IREAGSTALQELAFTVYDGVEYVEWARRRGLDVDEFGPRLSFFFNAHNDFFEELAKYR
                     AARKIWYRLMKDRFEAKNQRTSLMRFHT"
BASE COUNT          565 a          802 c          710 g          471 t
ORIGIN      
        1 aaccgtgcag cagcgcgccc tggatctcac caacaagacc ctttccgaca atcagaagca
       61 ggtgtccgcc gggacgctgg ctccacttgc cgtggtgcag tcgcagagtg ccgtggccac
      121 ggccaagcaa aacctgatca ctgcgcaggt gaatctccag ttgcaacaac tgctcatgaa
      181 aaatgcgatc acccgcaaca tggctgaccc catgctcgca gtcgcgcccg tgattccgac
      241 cgacacgctg agtaccaccg aggagtacga ggtgcggcca gttgaggacc tgatcgacga
      301 agccttgaag caacgtcccg agatcgccag tgcacgcctt actctcacca acaacgagat
      361 atctcggaag tcaattcgca accagttgct gcccacagtg gacgtatacg cgttctacgg
      421 cgccgccgcg ctggcgggac cgcagaaccc tctgtgcgtg aatcccaatg gctgcccgcc
      481 gccgggaacc attcccacca caggctacgg cgatgcgttc ggcaatctgt tcaacagcag
      541 cgccccggat aaaggggtcg gaattaacat caacattcca ctgcgtaatc gtcagatcca
      601 ggccgaccag gtgcgctcgg tattggaata tcgccaggcg cagatgagtc tgcagcaaac
      661 cgaaaacacc atccggctgc aagtgcggca ggcacagttc gccatgcaac agaactacgt
      721 gggactgcag gcgaccatag cggcacgcga ctacgccgca cagagcttgg atgccgagca
      781 gaaaaaactg cgcatgggcg cttccaccag cacgctcgta ttgcagtcgt cctctaacct
      841 gactcaggcg gagtcgaatg tcctcattgc cgccaccaac tatgagaagt cgaaggtgca
      901 gctcgattta tccaccgccg agactttggc caaactcggc attgacatgt cagacgcgga
      961 aaccgcccag gtgaaacatt cgccaaaggt gcccggcact gtgccagcga atactaacga
     1021 cctgacacaa cccaagttga cacccatcca ggttccggga gtgacgcccg cgcttcagcc
     1081 gggtacgcca ccgcaaactc cggaccaggc acagccgccg acaccgcctc aacggtagtc
     1141 tcttccgccc gccgagtggt gagtttcgct cggcgggagt tgacagggcc aaagtgacga
     1201 cttagtcttt tattagtttt gcagacgtgt tcaacaccgt cagattggcc ccgagcattc
     1261 ggggcctttc gcgtttaggc cgcgaagagc tgtcgctcca cgtctatgat gcgaactatc
     1321 tgagccgaaa atatgtcaag gattggaagc ttccaatcgc aaataggttt ttcctcgacg
     1381 gcatcgaacg ggacactgct gcttcttgct ccggttcccc acatctacta gcatgtaggg
     1441 ttcgcgttca agtggagtag ctcatgtctg atagccgcaa acctgacggg aaagttctgg
     1501 ccgaattacc ggtcaccgat ttgttcgaga gtggccgcca tccctccgag tcggagaagc
     1561 agtgggccga gaagacgctc gctccgagcc tggagaaggg gccggagaag ccgatcggct
     1621 cacccacggg cgtcaacgcc gacgagcacg gccacgcgcg ctacaccacc atctcgggcc
     1681 tgccgattcg ccgcctctat acccccgccg accttcctgc cgactggaag tacgaagatc
     1741 acctgaacta tcccggccag ccgcccttta cgcgcggcat tcacgcttcc ggataccgcg
     1801 gcaagatgtg gaccatgcgc cagttctcgg gctttgcttc cccggaggag accaacgagc
     1861 gctacaaata tctgctggcc cacggcggcg gcggcctatc cgtcgcgttc gatttgccca
     1921 cgctgatggg ttacgacagc gatcatccct tcagcgaagg cgaggtcggc aagtgcggcg
     1981 tggccatcga ttcgctggaa gacatggaga tcctctttga cggcattgac ctggagaaga
     2041 cgacggtctc catgaccatc aactcgccgg cgtcgatact gtgggccatg tatctggcgg
     2101 ttgccgagaa gcagggcgcc gattggaaga aaatctctgg caccatccag aacgacatcc
     2161 tcaaggagta catcgcgcag aaggaataca tctatccgcc ggcgcaatcg atgcggctgg
     2221 tgattgacac cttcgagttc ggctccatct tcacgccgcg cttcaacacc atctcgatca
     2281 gcggttatca cattcgcgaa gccggctcga ccgcgcttca ggagctggcg tttacggtct
     2341 acgacggcgt ggagtacgtc gaatgggcgc gccgacgcgg gctcgacgta gacgagttcg
     2401 gcccgcggct cagcttcttc ttcaacgcgc acaacgactt cttcgaggag cttgcgaaat
     2461 accgcgcggc ccggaagatc tggtatcggt tgatgaagga ccgcttcgag gccaagaacc
     2521 agcgcacctc gctgatgcgc ttccatac
//
LOCUS       DASRKK010000363         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_125213, whole genome shotgun sequence.
ACCESSION   DASRKK010000363 DASRKK010000000
VERSION     DASRKK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125213"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFC15_07995"
     CDS             complement(<1..519)
                     /locus_tag="VFC15_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10138.1"
                     /translation="MKQKRFSDALGLRLTRQALRVLKERGILAHSPVSLEHQYLAKRY
                     VVRGLESGGAAGELGRYVTFTQEDGQPIEWLHPVEAIGVNGLHAIVVAPVLVRVEMLR
                     TGCTYELLITQHRPGATNDGKRPPLETRLLFRGIHGRLDLDLSGKDKDKAGTALPTFY
                     SRAGEEVRIPKRF"
     gene            complement(541..1515)
                     /locus_tag="VFC15_08000"
     CDS             complement(541..1515)
                     /locus_tag="VFC15_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10139.1"
                     /translation="MATTIMATRQVVEDYQRLFVNRRAYTMQSLRPHPETGRHYYFRP
                     SKKGTDIPLILTDETIRQHLEGKITVGLYAINPSTQRCKWVAIDADYKNSMEDLLKLQ
                     YRLTQDKVEPALEMSKRGGHLWIFLATPLLAKECRIYIHDLALRLGVPVKGAGLAEGI
                     EVFPKHDAIGEGEFGNAIRGPLGIHRGADRRFWFYGADYTLEDQIPYLNRLRKVTEEE
                     LRRFIAGKERPEPDFSPRPKRGTSTLRKTWNGGSEFRILEHVGKVRKAGRNYVARCPA
                     CADSGHDRSGDNLAILIDDPRFYKCWAGCTKGMIRAALGCPIRVRHSA"
     gene            complement(1629..1994)
                     /locus_tag="VFC15_08005"
     CDS             complement(1629..1994)
                     /locus_tag="VFC15_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10140.1"
                     /translation="MMIEPVVQIGPAVDLQTAIAELIKVAENVEIVRWIELPASVLLF
                     LLVPGDPDSGAVYVLDRKKGTWYAVDFEDEQFGGYSVSQLEMLLRDCNFLDLIERPGL
                     WRAGLQWVVLPGMRPETAV"
     gene            complement(2678..>3016)
                     /locus_tag="VFC15_08010"
     CDS             complement(2678..>3016)
                     /locus_tag="VFC15_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10141.1"
                     /translation="LLQYAVNYNMKHGWYVTSSPILTANWRASSGNIWTVPFGGGVGR
                     IMQIGFQPVNISAQFFGNAAYPTGGSPWGMRVQLAFLFPKFTKAQEKEMLEQKLKQMD
                     QEQQQTQPQK"
BASE COUNT          597 a          895 c          830 g          694 t
ORIGIN      
        1 aaaccgcttc ggtatcctga cttcctcccc ggcgcgcgaa tagaacgttg gcagagccgt
       61 gccagctttg tccttgtcct ttccggacaa atccagatcc aagcgaccat gaatgccccg
      121 aaacaggagt cttgtttcca gtggcggccg ttttccatcg ttggtcgcgc ctgggcgatg
      181 ctgcgtaatc agcagttcgt acgtgcatcc cgtgcgcagc atctccacac gcaccagcac
      241 cggcgcaacc acgatggcgt gtaagccatt tactccgatg gcctccaccg gatgcagcca
      301 ctcgatcggc tgaccatctt cctgggtgaa agtgacgtat cgcccaagtt ctccggccgc
      361 gccgcccgac tccaacccgc gcacaacata ccgtttcgct aggtattggt gttctaggct
      421 gaccggcgaa tgggcaagaa tgcctcgttc tttcaagacc cgcagcgctt gccgcgttag
      481 cctcagtccc agtgcatctg aaaatcgctt ctgcttcatg acaacaacac tctccttctc
      541 tcaggcggaa tgccgtacgc gaatcgggca gccgagtgcg gcccgaatca tccctttggt
      601 gcagccagcc cagcatttgt aaaagcgggg atcgtcgatc aggatggcca ggttgtcacc
      661 gctccggtcg tgcccggagt ccgcgcaggc aggacagcgc gcgacatagt tgcgcccagc
      721 ctttcgtact ttcccgacgt gttccagaat gcggaattca gaacctccgt tccaagtttt
      781 tcgcagcgtt gaagtgcccc tcttcggacg cggcgagaaa tccggctctg gcctttcctt
      841 gccggcgatg aacctccgca attcttcctc ggtgactttt cgcaggcggt tgaggtatgg
      901 gatctggtcc tccagcgtgt aatcagctcc gtagaaccag aagcgacgat cggcgccacg
      961 gtggattccc aatggtccac gaatggcgtt gccaaactct ccttcgccga tggcatcgtg
     1021 cttgggaaac acctcgatgc cctccgccag gcccgcgccc ttcaccggca ctcccaacct
     1081 cagggcaaga tcatggatgt agattcggca ctctttggcc agcaaaggcg ttgcgaggaa
     1141 gatccaaaga tgcccaccgc gcttgctcat ctccagcgcc ggctccacct tgtcctgtgt
     1201 caggcggtac tgcagtttca gcaagtcctc catcgagttc ttgtagtcgg cgtcgatggc
     1261 gacccattta cagcgctggg ttgacgggtt gatggcatac aggcccaccg tgatcttgcc
     1321 ttccaggtgc tggcggatgg tttcatccgt cagtatcagg gggatgtctg tccctttctt
     1381 gcttggccgg aagtagtagt gccgccccgt ttccgggtgc ggtcgcaggg actgcatcgt
     1441 gtaggcgcgg cggttcacga acagcctttg gtaatcctcg acgacctgcc gggttgccat
     1501 gattgtcgtt gccatttgac tcgtcacctc cttctggcaa attcgcgaat ccgctcgctc
     1561 gtgttcgcca tttccccaca cagatttcgg atgtgggtgt cgttagataa cccggaatat
     1621 tctttctgtc aaacggccgt ctcaggcctc attccgggta gtaccaccca ctgcaaccct
     1681 gcgcgccaaa gtcctggccg ctcaatgaga tcgaggaagt tacagtccct caggagcatt
     1741 tcgagctggc tcacgctgta cccgccgaat tgttcgtcct cgaagtcgac ggcgtaccag
     1801 gttcctttct tgcgatctaa gacatagacc gccccagaat ccgggtctcc gggcacgagc
     1861 aagaacagca ggaccgacgc cggcaactcg atccaacgga cgatctcgac gttttcggca
     1921 acctttatca gttcggctat cgctgtctgc agatcgacag cggggccgat ctgcacaacg
     1981 ggttcaatca tcattcgtcc aaacattgca gtgatccttc ccgggcagtc cgcccaattc
     2041 gtgttgtgtt gaaggcaagc cgagaccaat ggcgcacacc tcggctcgcg cagtgagggg
     2101 gtaggtgtat tcaaggtgtt gttttgttgg ggcgcgctag ggtagggagt taggtgagag
     2161 caggacgaag atcgttaagc agggacgatc tctatatata aggatggaat tcgtacgctc
     2221 agattgatca ttactcggcg atgacgctaa atcgtaaatt cacagtcgcc gaccattcag
     2281 tagcgacccg ccagacgccc gggggtggga cagcccagtt cattagaagc gctgttttcg
     2341 tcaagtgacg tccaaggaac cgtatagcgg agggagcgca caaaattcgt ggacgctggc
     2401 tgacttagcg ggagtaatcg cccaacaggc cggtttcaag ccaacatcgg cattgccgac
     2461 cacatctacg tcagatccgg aactcctctc atcttgaccg acaccggtgc aattcatgcg
     2521 atgcagggtt ttcgacaatt ggggggcaat caggatagcc ggcgattcgc ttgtcttgcc
     2581 ccgtcatttc tcgtcgagag cacagaacag atacagagcc agcccaaccg cgttttccgg
     2641 atggttcttg aatcgagttt gattggttgg tcagggatca cttctgtggc tgtgtctgct
     2701 gttgctcctg gtccatctgt ttcagcttct gttccaacat ctccttctct tgcgccttgg
     2761 tgaacttggg gaacagaaag gcgagctgta cgcgcatgcc ccaaggcgag cctcccgtgg
     2821 gatacgcggc gttgccaaaa aactgtgcgg agatgttcac cggctgaaat ccgatctgca
     2881 tgattcttcc cacgccacca ccgaacggca cggtccatat gttgccgctg ctcgctctcc
     2941 agttggcggt gaggatgggc gacgaggtga cgtaccaacc atgcttcatg ttgtagttaa
     3001 cggcgtactg cagcag
//
LOCUS       DASRKK010000364        10447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_517182, whole genome shotgun sequence.
ACCESSION   DASRKK010000364 DASRKK010000000
VERSION     DASRKK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517182"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFC15_08015"
     CDS             <1..197
                     /locus_tag="VFC15_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10142.1"
                     /translation="DLDEFCRKLMQKGFYITQMPRMMPWGWRHAYLNDPDGHEISLYW
                     AGENRMKKTVMQAARAAAKG"
     gene            complement(287..514)
                     /locus_tag="VFC15_08020"
     CDS             complement(287..514)
                     /locus_tag="VFC15_08020"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZM10143.1"
                     /translation="MAKKSAPSESVYKLVEVIGTSSKSWEDAANTAVETAAKTLRDLR
                     IAEVVKMDMKVENGKVTEYRTRVLLSFKYQS"
     gene            complement(716..1240)
                     /locus_tag="VFC15_08025"
     CDS             complement(716..1240)
                     /locus_tag="VFC15_08025"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM10144.1"
                     /translation="MATATKANTMIEPMLGEFREEAGVTRRILERVPADKLTWRPHPK
                     SMSLGQLAIHIATVPGAIATLAQGDGLDVSQAAFEPPQPKSAQEIQAAFEESVRTAET
                     VLNGMTDETARGSWTLKRGDKEIFTQPRIGVMRSIMLNHWYHHRGQLSVYLRLLDVSV
                     PVVYGPTADENPFM"
     gene            complement(1387..1848)
                     /locus_tag="VFC15_08030"
     CDS             complement(1387..1848)
                     /locus_tag="VFC15_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM10145.1"
                     /translation="MSTITTAPGIDSLTLLVTQEIHVQASVEDTFEALLEQVGPGGTR
                     PDGTSMQMKIEPWPGGRWYRDLGDNNGHLWGHVQAIKRPTLLEITGPLFMSYAVVSNV
                     QYRLTEVDGGTLIQFHHKALGVIQDDHRQGVTGGWAHILEGIRQRAEAGSR"
     gene            complement(1873..2214)
                     /locus_tag="VFC15_08035"
     CDS             complement(1873..2214)
                     /locus_tag="VFC15_08035"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM10146.1"
                     /translation="MARAATTSDAFNAVAESRRRQILVHLMHAERSVGDIVVALGLEQ
                     PSVSKHLRVLRNVGLVWVRRNGKHMMYRTNAEAIRPLHEWTKTFERMWAHQLNSIKQR
                     AEAKNKERKSD"
     gene            2601..2690
                     /locus_tag="VFC15_08040"
     tRNA            2601..2690
                     /locus_tag="VFC15_08040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2635..2637,aa:Ser,seq:tga)
     gene            2760..4292
                     /locus_tag="VFC15_08045"
     CDS             2760..4292
                     /locus_tag="VFC15_08045"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZM10147.1"
                     /translation="MKTTAKIAVFDTTLRDGEQAAGTRLGSRDKIRLARQLARLKVDI
                     IEAGFPASSPEDFDAVRKIAQEIKGPTICALSRAVAADIESCGKALAKAARPRIHTGI
                     GVSDIHVMGKFRDERYGKTLEAKRAKLVVQAVDAVKRARNCVGDVQFYAEDAGRAEPL
                     YLFEMLEAVIDAGATVVNIPDTTGYTVPEQYGQLIRGIRDNVPNIARATISVHCHDDL
                     GLAVANSLAGIRNGARQVEGTINGIGERAGNAALEEIVMGIRTRADYYGVSTGVEPRE
                     FFRTSRMVADLLGMPVPANKAIVGLNAFAHSSGIHVDGFLKDRQTYEIMRPADVGVTG
                     SRVVLTARTGRAGLRSRLEKLGYQLSRQELDTMYQRFLVVADKKQEVLDEDLAALVHD
                     ALPPAEQNLKLEYLHSYSGTTAIPTATVRLLVNGKLKEGASIGDGPVDAVYKAIAGLT
                     ATHAKLLRYDIRAVTEGTEAMGEVTVHLEIGNRRAMGRGASTDIIEASAKAYVDGLNR
                     LG"
     gene            complement(4449..5912)
                     /locus_tag="VFC15_08050"
     CDS             complement(4449..5912)
                     /locus_tag="VFC15_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZM10148.1"
                     /translation="MNRLILAAGILASGLLAGCTVGPNYHRPPMPAPPAYKTEGPWRV
                     AAPKDAIPKGAWWEIYSDAELNNYEQQLLKANQSLSAAQDRLSQSRSLARVTSAGYFP
                     TLNTDPNASRTRYSGNRPEVVTIGRPLTQNTFEIPFLLNYEPDLFGKVRRTLEASNAS
                     LQSTAADYYNVILVLTAELAADYFSLRELDAETQVVQEGVTIQQEGLRLVDARHTGGV
                     ASGLDLAQQQTLLDSTQTQLYLVQQQRAQFEHAIAVLTGNAASTFSIPVLPLNATPPA
                     IPLGVPSDLLERRPDVATAERQMAYQNAQVGIATAAFYPQLTLFGGGGVQSTDITTLF
                     SAPSAIWAVGGDLLQPIFQGGRNRANLAAAKSAYDEAEANYRESVLTAFQQVEDGLSG
                     LNTLSQAVNTQSAAVADARHALELANNRYVGGVTSYLDVITAQSALLTNQRLATQLLG
                     QQMVTSVYLVKALGGGWDASQIQNEQVHPKAIQTIQQ"
     gene            complement(5909..7135)
                     /locus_tag="VFC15_08055"
     CDS             complement(5909..7135)
                     /locus_tag="VFC15_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM10149.1"
                     /translation="MDSHSSQTEASDDERQAPEITPPPKLPPAPPRKALLLIGLVLLL
                     LFVGGLITMLMRMRSSHVLADETERESIPTVAVIHPTAEKPDEELVLPGSLQAYEESP
                     VYARTNGYLLRWYKDMGSHVKQGELLADIDTPEVDQELMQVRANRQQIVAAMDLAKIN
                     ADRYVSLRKTDSVSQQESDQQSSGYQQAKANLDAADATVRRLEQLESFKHVYAPFSGV
                     LTKRNVDPGALINSGGTGKELFDIAKVDPLRVFVSVPQNYAPAIKNGMEAWVSLPELS
                     GEKFKGMVVRTAESIDPMTRTLLTEVDVPNRDGRLLPGSFGEVHFRIGINVQKLTIPV
                     NAMLFRQEGPRVAVVGSDGKVHLRPIVIGKDYGATLEILGGVEAGDRVIVNPADSIEE
                     GQQVNVAPENQGGQQS"
     gene            complement(7167..10325)
                     /locus_tag="VFC15_08060"
     CDS             complement(7167..10325)
                     /locus_tag="VFC15_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM10150.1"
                     /translation="MWIVALALRRPYTFIVMALVLLILTPIVILRTPVDIFPDINIPV
                     ISVVWNYSGFAPSEMADRIVTNSERGITITVNDIEHIESQSVNGVGVIKIFFRPQANI
                     QTALSQVTAMVQTVVRGLPPGTTPPLVISYSASSTPIVQLGMSSKTLPEQQLFDLGQN
                     FLRTQLATVHGAATPYPYGGKIRQVQVDLDIPRLQSNGLSPADIVNAINSQNLITPSG
                     SAKIGSLEYQVEMNSSPQTIAELNDLPVKTVNGSTIYMRDVAHVRDGFAPQTNIVRQD
                     GVRGTLMSIYKIGSASTLTIVAAVRSILPIAAQSLPKDVTIKPLFDQSLFVRASINGV
                     VREALIAAALTATMILLFLGDWRPTIVISISIPLSIFVSVIVLSALGETINIMTLGGL
                     ALAVGILVDDATVEIENIERNLAQGKEMKQAILDGAAQIAVPAFVSTLCICIVFVPMF
                     FLTGVARYLFVPLAEAVVFAMLASYFLSRTLIPTLVMYIMRGHEHRAAGPKTIFGRYQ
                     RGFEAGFEKFRRGYYQLLETTLGHGKVFAACFLAFCVLSMGLVFFLGEDFFPSVDAGT
                     MRLHVRARAGLRVEETARLCDEIEKYLHQQIPKDELVTILDNIGLPYSGLNLSYSNSG
                     VIGTSDAELLVGLNPEYHHPTAGYIRTLREELPKRFPGVEFFFQPADIVTQILNFGLP
                     APIDIQISGADMQGNYFIAQKIANRVKQIPGTADVHVQQMLTLPTLHLEIDRTKVTQV
                     GLNTRDVAQSVLVSLSGSFQTAPNFWLNPKNGVTYQVAVQSPQYRVTSLQDMMEMPIN
                     DPGAANSQMLSNLAQVTPTARPAVVSHYNVQPVIDVFVSTQDRDLGAVAADTMKVLKP
                     FEQNLPRGTRIVVRGQIETMQSSFLGLGIGLVMAIVLIYFLIVVNFQSWLDPFIIITA
                     LPGALAGICWILLLTRTRLSVPSLTGAVMCMGVATANSILMISFARDRMHAGIPAAQA
                     ALEAGYTRIRPVLMTALAMIIGMFPMALGLGEGGEQNAPLGRAVIGGLIFATVATLFF
                     VPTIYAMIHGHIEAKQAERKQS"
BASE COUNT         2156 a         3059 c         3097 g         2135 t
ORIGIN      
        1 gcgatctcga cgagttctgt cgcaagctta tgcaaaaagg cttctacatc acgcagatgc
       61 cgcgcatgat gccctggggc tggcgacatg cctacctcaa cgatcctgac ggacacgaga
      121 tcagcctgta ttgggcaggc gaaaaccgca tgaagaagac ggtgatgcag gccgcgcgcg
      181 cggcggcgaa agggtagaga tatccacctg cggaattccc aggcgagcgc cgaaaagcgc
      241 ggcgcgagcc tggggagccg gttgatggga acgcctgggc ggcgtctcag ctttggtact
      301 tgaaagacag cagcacgcgc gtgcgatact ccgtgacttt gccgttctcg accttcatgt
      361 ccatcttgac aacctcggca atgcgcaggt cgcggagggt ttttgcggcg gtttccacag
      421 ccgtgttcgc cgcatcttcc caggacttgg agctggtgcc gatgacctcc accagtttgt
      481 aaacgctttc gcttggtgca gatttcttag ccataatgcc tcgtattttc cgcggaacgt
      541 cagccggtgc gaccctgttg tcgatccggc atttccataa tgtcccggcg attacgaatt
      601 gtgggcgcat tgagcgggga aagcaactgg gcatctggag agtgagcgcg ggattcccaa
      661 gttggcgccg aggaaacgac acgaacctgg gaatccgggc aggcttgcat gttgcttaca
      721 tgaacgggtt ttcgtctgcg gtgggtccat agacaaccgg gaccgataca tccaacagcc
      781 ggaggtaaac cgacagttgt ccgcgatggt ggtaccagtg gttcagcatg attgaccgca
      841 tgacaccaat gcggggctga gtgaaaatct ccttgtcgcc acgtttgagg gtccaacttc
      901 cccgggccgt ctcatctgtc attccgttca ggaccgtctc agcggtgcgg acactctcct
      961 cgaacgcggc ttgaatctct tgcgcgctct tcggttgcgg cggctcgaac gcggcctgcg
     1021 agacatcgag cccgtctcct tgcgccagtg tcgcgatcgc gcccggcacc gtcgcgatgt
     1081 gaatcgccaa ctggccaagc gacattgact tgggatgcgg tcgccacgtc agcttgtcgg
     1141 cgggaactcg ttccagaatc cgcctggtca cgccagcttc ctcgcggaac tcgccaagca
     1201 tcggctcgat catagtgttt gccttcgttg ctgtagccat cgtcaatact cctttgctgc
     1261 aagttagttg ttgtcttcct ggcgcgaaat gccttcacca tcagagcgct cgaaccgcca
     1321 gtcgaaatcg cgccgcttac gatccatgcc gcggttgccg gacataccgg atggatcgcc
     1381 cgctcctcat cgcgaaccgg cttccgcccg ctggcgtatg ccctccagaa tgtgcgccca
     1441 accgccggtg acaccctggc gatggtcgtc ctgaattacc cccagcgcct tgtgatgaaa
     1501 ctggatcagg gtgccgccat ctacttcggt cagccggtac tgcacattcg aaaccacggc
     1561 gtaggacata aataacgggc cggtgatctc gagcagcgtc ggccgcttga tcgcctgcac
     1621 gtgtccccag agatgcccat tgttgtcgcc caggtcgcgg taccatcgac cgcccggcca
     1681 gggctcgatc ttcatctgca tcgacgtgcc atcgggtcgg gtgccgccgg ggccaacctg
     1741 ttccagcagc gcctcaaagg tgtcttccac cgaagcctgc acgtgaattt cctgggtcac
     1801 gagcagggtc aaactgtcga tccccggggc cgttgtaatc gtgctcatac ttcctcctct
     1861 tgtgtcttca attcaatcgc tcttccgttc cttgttcttg gcctcggcac gttgcttgat
     1921 gctgttcaac tgatgcgccc acatccgttc gaaggtcttg gtccactcgt gcagcggccg
     1981 aatcgcctcg gcattggttc gatacatcat gtgcttcccg ttcctgcgaa cccacaccag
     2041 ccccacattc cgcagcaccc gcagatgctt cgagacggac ggctgttcca accccagggc
     2101 gacgacgata tcgccaaccg accgttccgc gtgcatgagg tgaacgagga tctggcggcg
     2161 ccgggactcc gccaccgcgt tgaaggcgtc ggaagttgtc gccgctcgtg ccatgagggg
     2221 aactatatat tcctatatag gaatatgtca agtggtattt caaggtggga cattttttcg
     2281 cagatccccg agcgaagggt gtgcggtctg tttgaaattg ccgaagaatc cagcatgagt
     2341 accgatgctg gaacattcgc agactggttg gttgaagtgc gcaaacccct cgactcgatc
     2401 aacatgtcaa tggaaacgtg gcacgccgtt tggccaacgt ttctacgacg atgaacttgt
     2461 acgtgaaggc cgtcggagcg gatgctgtgg ccgcgatgca gtccttagat cgggttttat
     2521 gcgacggtta tgcaacggat ccggtttcgg gcatgaaagc agctgtcgta gaatgatgaa
     2581 aacacacatg ttcttcatgc ggagggatgt gtgagcggtt gaaacaggcg gtcttgaaaa
     2641 ccgttatacc cgaaagggta tcgggggttc gaatccctct ccctccgcca acaagtctaa
     2701 tgcgatcaca aaccgttctg ttccgataac aaatccgaaa catcgttgag gatgcgggaa
     2761 tgaaaactac tgcaaagatt gcggtgtttg ataccactct gagagatggc gaacaggcag
     2821 cgggaacccg cctcgggagc cgcgacaaga tcaggctggc acgccaactc gcccggctga
     2881 aggtggatat tatcgaggct ggcttcccgg catcgtctcc cgaggacttt gacgccgtcc
     2941 gcaagatcgc gcaggagatc aaagggccta cgatctgtgc gctgtctcgc gcggttgccg
     3001 ccgacatcga gtcctgcggc aaggcgctcg ccaaggccgc gcgtccccgc atccacaccg
     3061 gcattggcgt ttccgatatt cacgtcatgg gcaagttccg cgacgagcgc tatggcaaga
     3121 cgctcgaagc taagcgcgca aagctggtgg tgcaggccgt ggatgccgta aagcgcgctc
     3181 gtaactgcgt tggcgacgtt cagttttatg ccgaagacgc cggccgcgca gaacccttat
     3241 atcttttcga gatgttggag gcggttatcg atgcgggcgc aacggtggtc aacattcccg
     3301 acaccacggg ctacaccgtt cccgagcagt acggtcaatt gattcgcggc attcgcgaca
     3361 acgtgccgaa cattgcgcgc gcaaccatca gcgtccactg ccacgacgac cttggtctgg
     3421 cggtggccaa ctcgctggca ggcattcgca acggtgcacg ccaggtagaa gggacaatca
     3481 acggaatcgg cgagagggcc ggtaacgccg cgctggaaga aatcgtgatg ggcatccgta
     3541 cccgcgccga ctactacgga gtgagcaccg gagtcgaacc gcgcgagttc ttccgcacca
     3601 gccgcatggt cgccgacctg ctcggaatgc cggtccccgc caataaggcc atcgtcggat
     3661 tgaacgcgtt cgcgcatagt tccggcatcc acgtggacgg gttcctgaaa gaccggcaga
     3721 cctacgaaat catgcgtccc gccgacgttg gagttaccgg cagccgcgtt gtcctgacag
     3781 cacgtacggg ccgggccggg cttcgctcac ggctggagaa actcggctac caactctcca
     3841 ggcaggagct tgacaccatg taccagcgct tccttgtagt ggcggacaag aagcaggaag
     3901 tgctcgacga agacctggcc gcgctggttc acgatgcctt gcctccggcc gagcagaacc
     3961 tgaagctgga gtacctgcac agctacagcg gcacgacggc cattccgaca gcgactgtgc
     4021 gcctgctggt aaacggcaag ctaaaggaag gagcttcgat cggcgacggg ccggtcgatg
     4081 ccgtgtacaa ggcgattgcg ggactcaccg cgacccacgc gaaactcctg cgctatgaca
     4141 ttcgagccgt aaccgaaggc accgaagcaa tgggagaggt gacggtacac cttgagatcg
     4201 gcaaccggcg cgccatgggt cgcggtgctt ccaccgacat cattgaggcc agcgcaaagg
     4261 cgtatgtgga cggcctgaac cggctgggct gatttcgagc gggctaacaa caatatttcg
     4321 atgaacttca aaatcggacg gccgaatgcg gatacatttc gcgcgcagcc ttgaaaaggt
     4381 ttcctgcaat aagagccaaa tgattcaagt tgaatccgga aaacggacac aaaggtgatg
     4441 cggggtactt attgctggat ggtctggatc gccttgggat ggacctgttc attctgaatt
     4501 tgcgatgcat cccaaccccc accaagtgct ttgacaagat agaccgaggt gaccatctgc
     4561 tggcccagga gttgcgttgc caggcgctgg ttggtcagca gcgccgattg cgcggtgatg
     4621 acgtcgaggt agctggtgac tccgccgaca tagcggttgt tggcaagttc caacgcgtga
     4681 cgggcatcag ccacggctgc gctttgcgtg ttgacggcct gcgacaacgt gttgagaccg
     4741 gagagtccgt cttccacctg ctggaatgcc gtcagcaccg actcgcgata gtttgcttct
     4801 gcctcgtcat aagcagattt cgccgccgcg aggttggcgc ggttgcgccc tccctggaaa
     4861 atcggctgca acaaatcgcc tcccaccgcc cagattgcgc tgggagccga gaagagggtg
     4921 gtgatgtcgg tgctttgaac tccacctcct ccgaagaggg tgagctgcgg atagaaagca
     4981 gcagtggcga tgcctacctg cgcattttgg taagccatct ggcgctcggc ggtagcgaca
     5041 tccggacgcc gctccagcag atcggacggc acgcccagtg gaatcgcggg cggagttgca
     5101 ttcagcggaa gcacaggaat gctgaaggtc gaggccgcat tcccggtaag tacggcgatg
     5161 gcgtgttcaa actgtgcgcg ttgctgttgc accaggtaga gctgcgtctg ggtggaatcg
     5221 agcagcgtct gctgttgcgc caaatcgagt ccggaggcca ctccgccggt gtgtcgagcg
     5281 tccaccagtc gcaatccctc ttgctggatc gtgacccctt cctgcacgac ttgggtttcg
     5341 gcatcgagct ctcgcaggct gaagtagtca gcggccaatt cggctgtgag caccaggatc
     5401 acgttgtagt agtccgccgc cgtcgattgc agcgatgcgt tggatgcctc gagagtgcgc
     5461 cgcactttcc cgaacagatc aggctcgtaa ttcagcagaa atggaatctc gaaagtattc
     5521 tgcgtgagtg gcctgccaat agttacgact tccgggcggt tccctgaata cctggtacgc
     5581 gaagcattgg gatcggtgtt cagcgtggga aagtatcctg ccgatgtgac ccgcgcgagc
     5641 gagcgagact gactgagacg gtcttgggct gcgctgagtg actgattggc tttgagtagc
     5701 tgctgttcgt agttgttcag ctcggcgtcg ctatagatct cccaccatgc gcctttggga
     5761 atcgcgtcct tgggcgcggc gacgcgccat ggtccttccg tcttgtaggc tggcggagcc
     5821 ggcatgggcg gacgatggta gtttggtccc acggtgcatc ctgccaacaa gccgctggca
     5881 agaatgcccg ccgccaggat caaacggttc acgactgctg tcctccctgg ttctcgggag
     5941 cgacattcac ttgctggcct tcctcaatgg agtcggcagg attgacaatc acgcggtccc
     6001 ccgcctccac acctccgagg atttccaggg tcgcgccgta gtctttgcca atcacgatcg
     6061 gccgcaggtg gaccttgcca tcgctgccga ctacggccac acgcgggcct tcctggcgaa
     6121 acagcatcgc attgaccggg atcgtcaatt tctgcacatt aatgccgatt ctaaaatgca
     6181 cctcgccgaa cgagccgggc agcaagcgcc cgtcgcgatt gggcacgtcc acctccgtga
     6241 gcaaagtccg cgtcatggga tcgatcgact ccgccgtgcg cacgaccata cccttgaatt
     6301 tctcgccaga caactctggc aaactgaccc atgcttccat cccgttctta atggccggcg
     6361 cataattttg cgggacactc acgaacaccc gcagcggatc gactttcgca atatcgaaaa
     6421 gctccttgcc cgtgcctccc gagttgatca gagcgccggg atctacattg cgcttggtca
     6481 gtacaccgga gaacggcgcg taaacatgtt taaaggattc caattgctcg aggcgccgga
     6541 ctgtcgcgtc cgccgcatca aggttggctt tggcctgctg atacccgctg gattgctggt
     6601 ccgactcctg ttgcgaaact gagtccgtct tgcgcaagct gacatagcga tcggcattga
     6661 tcttggccaa atccatcgcc gccacaatct gctgccggtt ggcgcggacc tgcatcagtt
     6721 cctgatccac ttcaggagta tcgatatcgg ccagcagttc gccctgtttg acgtggctgc
     6781 ccatgtcctt gtaccagcgc agcagatagc cattggtgcg cgcgtaaacc ggcgactctt
     6841 cataggcctg caacgagccc ggcaacacca gttcctcgtc gggcttttcg gcagtgggat
     6901 gaatgacagc caccgtggga atcgattccc gctcggtctc gtccgccagc acatggctgc
     6961 tgcgcattcg catgagcatg gtgatcaaac caccaacaaa cagcagcagc aacacaaggc
     7021 caatcaacag cagcgccttg cgcggaggcg ccggcggaag tttgggcgga ggggtgatct
     7081 ccggcgcctg gcgttcgtcg tccgatgctt cggtctggct tgaatgcgaa tccatacgaa
     7141 ttggttccac ttcagttctt atcattctat gactgtttcc gctcagcttg cttggcttcg
     7201 atgtggccgt gaatcatggc ataaatcgtg ggcacaaaga acaaagtcgc cacggttgca
     7261 aagatcagac cgccgatcac ggcgcgcccg agtggggcat tctgctcgcc gccttcaccc
     7321 agtccgagcg ccatcgggaa cataccgatg atcatcgcca gcgccgtcat caggaccgga
     7381 cgaatacgcg tgtaaccagc ctcaagtgca gcctgagcgg cgggaatccc cgcgtgcatc
     7441 cggtcacggg caaaagaaat catcagaatg gagttggccg tggccacccc catgcacatc
     7501 accgctcccg tgagcgacgg gacactcaag cgggtgcgcg tcaagagcag gatccagcag
     7561 ataccggcca gcgccccggg cagcgccgtg atgataatga acggatcaag ccaggactgg
     7621 aagttcacca cgatcaggaa gtagatgagg acgatggcca tcaccagtcc aattcccagt
     7681 ccgaggaatg acgactgcat ggtctcgatc tgtccacgaa ccacgatccg cgtaccgcgc
     7741 ggcagattct gctcgaaggg tttaaggacc ttcatcgtgt ccgcggccac ggcccccaga
     7801 tcacggtcct gggtgctgac gaaaacgtcg atcaccggct gcacattgta atgggaaacc
     7861 accgcgggtc gcgccgtcgg cgtcacctgc gccaggttgc tgagcatctg agaattggcc
     7921 gcgcctgggt cattgatcgg catctccatc atgtcctgca acgaggtcac gcggtactgc
     7981 ggagattgca ccgcaacctg ataggtcacg ccgttcttgg gattcagcca aaaattcggc
     8041 gcggtctgga agctgccgct cagcgacacc agcacactct gcgccacgtc gcgagtgttc
     8101 aggccaactt gcgtgacctt agtgcggtca atctcgagat ggagcgtcgg caaggtcaac
     8161 atctgctgca cgtggacgtc ggcggtaccc ggaatctgct tcacccggtt cgcaatcttt
     8221 tgcgcgatga agtaattgcc ctgcatgtcc gcgcctgata tttgaatgtc aatgggcgcc
     8281 ggcaatccga agttcaggat ctgcgtcacg atgtcggcag gctgaaaaaa gaattccaca
     8341 cccgggaagc gcttgggcag ttcctcgcgc agggtgcgga tatatcccgc ggtggggtgg
     8401 tgatactccg gattcaatcc caccaagagt tccgcgtcgc tggtaccaat cacgccggag
     8461 ttgctatagg aaaggttgag gcccgaatag ggcagcccaa tattgtccag gatcgttacc
     8521 agttcgtctt tggggatctg ctggtgcagg tacttctcga tctcgtcgca gagccgggcc
     8581 gtctcttcca cgcgcaggcc ggcacgtgcc cgcacatgaa gccgcatcgt gccggcatcc
     8641 accgacggga agaaatcttc acccaggaaa aacaccagtc ccatcgatag cacgcaaaac
     8701 gcgaggaagc acgcagcgaa cactttgccg tgtccgagag tggtttccag aagctggtaa
     8761 tagcctcggc gaaacttctc gaaaccagcc tcgaaccctc gctggtagcg tccgaagatc
     8821 gtcttagggc cggcggcccg gtgctcatgg ccgcgcatga tgtacatcac cagggtcgga
     8881 atcagcgtgc gcgacaggaa atacgaggcc agcatcgcga acaccacggc ttcggccagc
     8941 gggacgaaaa gataacgggc gacgccggtg agaaagaaca tcggcacaaa cacgatgcat
     9001 atgcacagcg tcgaaacgaa cgccgggact gcaatctgtg ccgcgccatc aagaatcgcc
     9061 tgtttcatct ccttgccttg ggcaaggttt cgctcgatgt tctcgatctc aacggtcgcg
     9121 tcgtcaacca atatgccaac cgcgagagcc aggcctccga gcgtcatgat gttgatggtt
     9181 tcgcccagag cgctcagcac gatcaccgac acgaatattg acagcgggat cgagatggag
     9241 atcacgatgg tgggacgcca gtcgcccaga aacagaagga tcatggtcgc cgtgagcgct
     9301 gccgcgatca aggcctcacg gacgacgccg ttgatggaag ctcgcacaaa cagcgactgg
     9361 tcgaacagtg gcttgatggt gacgtctttg ggaagcgact gcgcagcgat cggtaatata
     9421 cttctcaccg cggccacaat ggtgagcgtg gacgcgctgc cgatcttgta gatcgacatc
     9481 agggtgccgc ggaccccgtc ttggcgcacg atgttcgttt gtggtgcgaa tccgtcgcgc
     9541 acgtgcgcga catcgcgcat gtaaatggtc gatccgttca ccgttttcac aggcagatcg
     9601 ttcagctccg caatggtctg cggcgagctg ttcatttcca cctggtattc cagcgaccct
     9661 atcttcgcgg atccggacgg cgtaatcagg ttctgcgagt tgatggcgtt gacgatgtca
     9721 gccggggaca gtccattgga ctgcagcctc gggatatcca agtccacttg cacctgtcgt
     9781 attttgccgc catagggata gggcgtggct gcgccgtgca cggttgccag ctgggttcgc
     9841 aggaaattct ggcccaagtc gaacagttgc tgctccggca gggtcttgct gctcatacca
     9901 agttgcacaa tcggggtgga cgatgcagag taggagatga ccagcggcgg cgtggttccc
     9961 ggtgggaggc cgcgcaccac ggtctgtacc atcgccgtga cctgagacag cgctgtctgg
    10021 atgttggcct gcggacggaa gaaaatcttg atcactccca caccgttcac cgactgcgac
    10081 tcaatgtgct cgatgtcgtt gactgtgatc gtaatgccgc gttcggagtt ggtcacgatg
    10141 cggtcggcca tttcgctggg cgcgaagccg ctgtagttcc acaccacgct gatcaccggg
    10201 atgttgatat cggggaagat atcgacgggg gtacgcagga tcacgatggg cgtcagaata
    10261 agcagcacca gcgccatgac gatgaaggtg tagggacgcc tcagcgcaag tgcaacaatc
    10321 cacatcggtc gggcaaaact cccatcgagg ccggggtgat gatacggcgg tcaacgccgg
    10381 cgcagctacg gtacaaattt acgattcgat acgatacgta gtggattcaa tatatgctat
    10441 cttggct
//
LOCUS       DASRKK010000365         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_125486, whole genome shotgun sequence.
ACCESSION   DASRKK010000365 DASRKK010000000
VERSION     DASRKK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125486"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFC15_08065"
     CDS             <1..518
                     /locus_tag="VFC15_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10151.1"
                     /translation="WARTIAAAQHGDVEATRSNAQKFDAMEEETRKTKYAYTLDGPDF
                     PRGEVHAWLAFAEKKSDDALRQMREVADLQDKVGKREVDIPAREMLADMLLELNRPQE
                     ALAEYESALKIDPNRFNGLAGAARAAEVAHQTGKANSYYAQLLKNCNNGKNSDRPELG
                     RAKTMLSKNGM"
     gene            complement(751..2076)
                     /gene="ltrA"
                     /locus_tag="VFC15_08070"
     CDS             complement(751..2076)
                     /gene="ltrA"
                     /locus_tag="VFC15_08070"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM10152.1"
                     /translation="MSLTTPFSVQKLQTALHAKAKESPNFRFHALYDKVDRNDVLVYA
                     YERCQANGGAAGVDNQTFENIEAYGRERWLDELTQELKSRTYQPQPVRRVYIPKPDGK
                     QRPLGIPTIRDRVVQTSAMLVLEPIFEADLPTEQYAYRADRSALDAVQHVHKRINTGH
                     DQIVDADLSSYFDSVPHAELMRSVARRVVDGAMLHLIKMWLEAPVEETDERGNKRRST
                     RNRDEGRGTPQGSPISPLFSNLYMRRFVLGWKKLGHEKRWQAYIVNYADDLVICCRVG
                     AEKALTEMRMMMSKLKLTVNESKTRVCYLPEEKFDFLGYTFGRCYSMRRGRVYLGTVP
                     SKKRVQRICRAIRDETGRDTVWQDAKTIVERLNRMMIGWANYFCLGPVSKAYSAVDMH
                     AHSRLRRWLCDKHKEPRPGYKRFPEEALNSVYGLVQLPHRTANLPWAKA"
BASE COUNT          578 a          920 c          792 g          668 t
ORIGIN      
        1 actgggcgcg gaccatcgcc gctgctcaac acggtgacgt ggaagccacc cgcagcaacg
       61 cgcagaagtt cgatgccatg gaagaagaga cgcgcaagac gaagtacgcc tacaccctcg
      121 acggaccaga cttcccgcgc ggagaggtcc atgcctggct ggcgttcgcc gagaagaaga
      181 gcgacgacgc gctgcgccag atgcgcgagg tggcggatct gcaggacaaa gtggggaagc
      241 gcgaggtaga cattcccgcg cgcgagatgc tggccgacat gctgctcgaa ctcaaccggc
      301 cgcaggaagc actggccgaa tacgagagcg ccctgaagat cgatcccaat cgctttaacg
      361 gactcgcggg cgccgcccgg gccgccgaag tggcgcacca aactggcaag gcgaactcct
      421 actacgccca gttgctcaag aactgtaaca acggaaagaa ctcggatcgc cccgaactcg
      481 gccgcgccaa aaccatgctg tcaaagaacg gaatgtagcc gtggcgcact acacactcga
      541 aaagcggcga tcgaggaaaa gaatgccgca cagaattacc gacgtatggc gagtagagtc
      601 gagaacaggc gcagtgactg tagtctcccg gtggatattc ggcgtggctt tcgccgcgcc
      661 acctcagtcc ggtttggctt ggtcctgttt cctgttcccg ctcatcgaac cggactggca
      721 gatttcccgc atccggctct cggaaaagac tcacgctttc gcccacggaa ggttcgcggt
      781 ccggtgtggt aactggacca aaccatacac agagttcaga gcctcttcgg ggaagcgctt
      841 atatcccggc cgcggctctt tgtgtttgtc gcataaccac cgacgcagcc tgctgtgggc
      901 atgcatatcg accgcactgt aggctttgct gactggcccc agacagaagt agttggccca
      961 accaatcatc atccgattga gcctttccac gattgtcttc gcatcctgcc agacggtgtc
     1021 tcgtccggtc tcgtcgcgga tcgcgcgaca gatgcgctgc actcgtttct tcgagggtac
     1081 cgtgcctaaa tatactcggc ccctcctcat cgagtaacac cgcccgaacg tgtatcccag
     1141 aaagtcaaac ttctcttccg gcagataaca gacccgcgtt ttgctctcgt tcaccgtcag
     1201 tttcagcttc gacatcatca tccgcatctc ggtcagagct ttctccgctc caactcgaca
     1261 gcagatgacg aggtcatcgg catagttcac gatgtacgct tgccaacgct tttcgtgccc
     1321 gagtttcttc caccccagca cgaaccgtcg catgtacaga ttgctgaaca gaggactgat
     1381 cggagaccct tgtggagtac ctcgaccttc gtcccgattg cgcgtactcc gacgtttgtt
     1441 cccacgctcg tcggtttcct cgaccggggc ttccagccac atcttgatca gatgcagcat
     1501 cgctccgtcg acgacccttc gagccaccga cctcataagc tcggcatggg gaacactatc
     1561 aaagtagctg ctgagatccg catcaacgat ctgatcatgc cccgtgttga tccgcttgtg
     1621 gacgtgttgt actgcgtcca acgcgctgcg gtctgcccga taggcatatt gctccgtcgg
     1681 caagtcggcc tcgaagattg gttcgaggac cagcatggcc gaggtttgca caacccgatc
     1741 ccggattgtc ggtatgccta acggtctctg cttcccatcc ggcttgggta tgtaaacccg
     1801 ccgcacaggt tgcggttgat aggttcgact tttcagctct tgcgtcagtt cgtccaacca
     1861 ccgttccctg ccgtatgctt cgatgttctc gaatgtctgg ttgtccactc ccgctgcccc
     1921 gccattggct tggcagcgtt cgtaggcata gaccagtaca tcgttccgat ccaccttgtc
     1981 gtacagcgca tggaaacgaa agttgggcga ttccttcgct ttggcgtgca acgccgtctg
     2041 caacttctga acactgaatg gagttgttag gctcatcgcc aatccctcag tcctttgtcg
     2101 cttcttgcgt tcgtctttaa ctaaggcccc ttccctccac cgacattacc ggcttcagcg
     2161 gtactgcgag cctttccgtc accccatacg gcctggcctg tctctcgcga gttgccagtt
     2221 gatcgatccc atgatcaccg ctggggcttc ccgtgttgcg tctggtctcc tatgcgtgca
     2281 tgccgtcgct attaccccgg caggttcaat ggagcttgtt cgctcgtctg tctccatcga
     2341 ctgcggcctt ccccctgaaa cagtaaggtc ggctcctgca attgtttttt cggggccttc
     2401 tcagcgttca ctcccgttac ggcctgtacg ctcgcggagt cgcctagcga ccctttacac
     2461 cgagagctcc gacagctttg ttgcctccgc tgccgtctcg attgcttccg ggtggagcga
     2521 accagttccc gggcgggagt ttcagccgct ggagtccagc gcctttcacg gcgcactgtt
     2581 acgccaacta tcgcgaatac ttcctctccg agcggagcaa attcgtagta tgctcatcgc
     2641 gttcgcagcc caaatcgcta ggaggactgt tggccgtcaa atcgagtcgc ccgacctacg
     2701 cgatcatact ggtggcggtt tctacccttt ggatgtctac catttttctc ccgcttttgt
     2761 attcggttgt gcttccgaac agtgatctcc aagggattgt ctccgctttg gaagggcacg
     2821 gaacagcagc cccagaggcg gctggcatgg ttcaggagac cacagccgca gtcgggccgt
     2881 taacaagggc tataaaagtg ggatactatt ccggggttac agctaccgtt cgagtcagcg
     2941 gatcgaatac gtccgaga
//
LOCUS       DASRKK010000366         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_712841, whole genome shotgun sequence.
ACCESSION   DASRKK010000366 DASRKK010000000
VERSION     DASRKK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712841"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1658
                     /locus_tag="VFC15_08075"
     CDS             282..1658
                     /locus_tag="VFC15_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM10153.1"
                     /translation="MTDSIATECLLFPDIFAKPVVLEFDQRQGSSDGGAVLLKAAETR
                     YGLIASMSGCLCDPRQPGKVDHSLPDLFAQRVFSIGCGYADANDSARLAADPMHKMLL
                     GRDPITGLDMASQPTLSRFENAVGPRQLYKIGAALAESVIGRHAQRLHHRARLVTIDL
                     DPTDDPTHGAQQLSFFNSHYDNACYLPMMGFVTFNDEAEQYLCAAVLRPGNVTAAAGA
                     VGILRRLTTMIRTSFPKTRIRIRLDGGFANPELLAFLDSEPGVEYVVAMASNAVLNRK
                     AEEDMEVARLLAGLTDQTEHVYGETHYAAGSWKRERRVIIKAEVVRAEDKAPKENPRF
                     VITNMKQTPQWLYEEVYCQRGEIENRIKELHAMEIDRTSCSGFWANQFRVLLTAAAYV
                     LMQELRLSAAGTNCARAQVWTLRERFLKLGARVMISVRRMVVHLPQSFPFLATFRHIA
                     LELGASPG"
     gene            1962..2357
                     /locus_tag="VFC15_08080"
     CDS             1962..2357
                     /locus_tag="VFC15_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10154.1"
                     /translation="MDSKITSPIPEPIVQLQRQLDQFRSTRPHRTKLPEPLWEAAVEL
                     ARQHGLYSVAHPLRLDYVQLKKRLGGVPSLRKKAAPAFVELITAPPTLPECLIEFESS
                     IGGKMRIQWKGSAAPDWASLLRAWREAER"
BASE COUNT          519 a          661 c          673 g          511 t
ORIGIN      
        1 ttgaggccgg ttgctatcag gacgagtaca acccgattca gtatggctag ttcgcaagga
       61 tcagatgaat actggccttt ccactatcct cagggcaaca ggctcgtttt cagaagagaa
      121 atccgggatt acgagctccg aggattttct gaagcgcgtc gtcgttgcgc aaatggcaga
      181 gcgctagccc ggataattca tgaaggttga gttagcgaaa gccttttgtg ctacaaacat
      241 gttgacaaga cagtgtttaa ggcacattga gaggctttcg cttgaccgat tctatcgcga
      301 cagagtgttt gctgtttcca gatattttcg ccaaacccgt ggtgcttgaa tttgaccagc
      361 ggcagggcag ttccgatggg ggtgctgtgc tgctaaaggc agccgagacc cgctacggat
      421 tgatcgccag catgtctggc tgtttatgtg atcctcggca gccgggcaag gttgaccatt
      481 cattaccgga tctttttgcc cagcgcgttt tctccattgg ctgtggttat gccgacgcca
      541 acgactcggc tcgattggca gccgatccta tgcacaagat gttgctcggt cgcgatccga
      601 ttaccggact tgatatggca tcgcagccga cactatcgcg ttttgaaaat gcagttggtc
      661 cacgtcagct ttacaagatt ggggcagcgc tcgccgagag cgtgatcgga cgccatgcgc
      721 aacggctgca ccatcgcgcc cggctcgtca ctatcgatct tgatccgacc gacgatccca
      781 cccatggggc gcaacagttg tccttcttca acagccacta cgacaacgca tgttatctgc
      841 cgatgatggg cttcgtcacc ttcaacgacg aggccgaaca gtatctgtgc gccgccgtgt
      901 tgcgtcccgg caatgtaaca gcggcggcag gcgcggtggg catcctgcgt cggttgacga
      961 ccatgattcg cacctcattc cccaagactc gcattcgcat tcgtctcgac ggcggcttcg
     1021 ctaatcccga actgttggcg tttttggata gcgagcccgg cgtcgaatat gtagtggcga
     1081 tggccagcaa tgcggtattg aatcgcaagg cggaagaaga catggaggtt gcccgcctat
     1141 tggccggtct caccgatcag acggagcacg tctacggcga aacccactat gcggccggta
     1201 gttggaaacg agaacgccgc gtcatcatca aggccgaagt tgttcgcgcc gaagataaag
     1261 cgccgaagga aaacccgcgt ttcgtgatta cgaatatgaa gcagactcca cagtggctct
     1321 acgaagaggt ttactgccag cgcggcgaaa tcgagaaccg gatcaaggaa ttacacgcca
     1381 tggagataga tcgcaccagt tgcagcggct tttgggccaa tcagttccgt gtgttgctga
     1441 cggccgcagc ctatgtgctg atgcaggagc tgcgcctgag cgctgctggc accaattgcg
     1501 caagggctca agtctggaca ctgcgggagc gcttcctgaa actgggagcg cgtgtgatga
     1561 tctcggtacg gcgcatggtt gtgcacctgc cgcaatcgtt tccgtttctg gcgaccttcc
     1621 ggcacatagc gctggaactg ggagcatcgc caggctagaa tcgcaccaca ccaccgaaag
     1681 gcacctcaat aggcttgacg caaccgacaa cagaggaaag ctctgtcccg aaaccaccgc
     1741 cgcgcacccg ccagaagcca atcagtctcg tcgccacatc caacagcggc aatcacgcag
     1801 ccgtcgccat cttcagcaac ctcgtcccgc ccaaaagaca gttcactctg tgttcatgaa
     1861 taatgcaggc taagactcca ataggtgact tttcggcaag cgtgcgtgac gccatctgag
     1921 tcgcgttgct aagttcaatc agccgtcgag gtcggctgag attggacagc aaaatcacat
     1981 cccccattcc cgaaccaatc gttcagcttc aacgccaatt ggatcagttt cgcagcacgc
     2041 ggccgcatcg gacgaagctg ccggagccgc tatgggaggc tgctgtagaa ctggcgcggc
     2101 agcacggttt gtattcggtt gcccacccgc tgcgactgga ttatgtgcag ttgaagaagc
     2161 gactcggtgg agttccgagc ctccggaaga aggccgcgcc tgccttcgtc gaacttatca
     2221 ccgcgccgcc gacgctgccg gagtgcttga tcgagttcga gtcttcgata ggtggcaaga
     2281 tgaggattca gtggaaaggt tccgctgcac ccgattgggc aagccttctt cgtgcttggc
     2341 gggaagcgga acgatgatcc atgt
//
LOCUS       DASRKK010000367        10192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_614197, whole genome shotgun sequence.
ACCESSION   DASRKK010000367 DASRKK010000000
VERSION     DASRKK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614197"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..631
                     /locus_tag="VFC15_08085"
     CDS             371..631
                     /locus_tag="VFC15_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10155.1"
                     /translation="MDGLERRRPKSLGYRSEAASVGGLFRYFDYFISAVRQVECHREH
                     LHPGYALIGGPRAFFRQFLNDCLNEMVAWTTTTATTAAVLLA"
     gene            1077..2309
                     /locus_tag="VFC15_08090"
     CDS             1077..2309
                     /locus_tag="VFC15_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10156.1"
                     /translation="MELLHRRCCGLDVHKETVVACLRLVSNGKVTTEVRTFQTTTADL
                     LHLSEWLAANDCMHVAMEATGVYWKPVWHILDDGEFELVLANAAHVKNVPGRKTDVND
                     AMWLAELLAHGLIRASFVPDTQTQEMRNLMRTRKQLVREQSSHVLRVQKTLEDANIKL
                     DSVLTDLMGKSGRAMIEALIAGETNPAKLASLADRRAKASHEEFREALRGRVTKPHRF
                     LLRLHLNQIDALDAAIATLDAQVEANLGPFRTAVDLIISIPGIKNLGAHVIVSEIGID
                     MSRFPSHAHLISWAGICPRNDESAGKRRSNRLRKGAPWLKTTLVQCAWAAVKKKNSYL
                     QAQFYRIKARRGPKKAIMAIAASILTAIYHMLKDGTMYKDLGCNHFDRRSTDQQKQRL
                     VKRLADLGYAVEIKPLAA"
     gene            2396..3376
                     /locus_tag="VFC15_08095"
     CDS             2396..3376
                     /locus_tag="VFC15_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10157.1"
                     /translation="MVDDTGRIVREVKVASEPEALLPVLKNPAYRFKRIGLEAGPLSQ
                     WLFSALAEAGLPVICVETRHMRAVLKAQINKTDRNDARGMAQMMRAGLYRPVHVKTLR
                     SQKLRMLLTHRKLLQSKAIAIDNDLRGTLRNFGLKVGVVGTVKFEARIRELVENLPDL
                     AGLVEPLLKVRRVLREQFSILHRRLLAIVRDDEVCRRLMTVPGVGPVVALTYRATVDV
                     PARFRNSKAVGAVFGLTPSRYQSGEINRAGAISKCGDEMMRVMLYEAAQVMLTRTNKW
                     SWLKAWAMQIARRRGMKKAIVALARRLAVIMHRIWVDGTEFRWTREAAVA"
     gene            complement(3804..4571)
                     /locus_tag="VFC15_08100"
     CDS             complement(3804..4571)
                     /locus_tag="VFC15_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10158.1"
                     /translation="MPTINSARYIDIILSYYRDMNIPVTVFVDSKTTDETAAIAARYA
                     HEVITFKNPATRVGEMIEGMSRLCRTRWILRIDDDELPSIRMMEFVRGVISCKKYHVV
                     GFNRYQCIFNCSGDLYQNLVHDPHTHRQWRLYRPDKVTFHGRGHSPGFDFEYPRGLNA
                     PLESFLIHLDWVVHSREERMEKLTRYDAHTPGHGMPFKSYYLADESAGFREELRPLPS
                     LEFDQIGRQLVARFPESAVLMKALNAADAACALRRDH"
     gene            4930..5565
                     /locus_tag="VFC15_08105"
     CDS             4930..5565
                     /locus_tag="VFC15_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10159.1"
                     /translation="MQDNANFELPTLKRIPWNKGKLTGAKPPLRQKHVWAIRTKLQID
                     QRPRDLAMFNLAIDSKLRGCDVVAIKVEDVAPNGYAVDRATVRQKKTGRPVRFELTEQ
                     TRQAVDDYIRTAGKKPGGFLFTARRGQEDRMTTRQYARLLSEWIAGIGLDPHLFGTHS
                     LRRTKATLIYRRTGNLRAVQLLLGHTKIESTVRYLGIEVDDALSIAEQVDL"
     gene            6004..7704
                     /locus_tag="VFC15_08110"
     CDS             6004..7704
                     /locus_tag="VFC15_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10160.1"
                     /translation="MTRFTPSTSKSEAEAIVRQWIDACVWRQEAHLAETGGIAFLETD
                     EIEKLGREDAAELDALLWFADRKHADDEKAQIARALGPVGPGLEAFGQVIDAAGRTMG
                     VPVGRQTVDGRLSARTFLRGYQSLLDELRETVAAIPRQVQAVVAKPVLPDFDFLTYWD
                     EFSATKQSNKDWKADTAAGADATPRLFRSLVGDLPINKIDGGVAGEFRLEYLKLPFDY
                     YHNKRWKKLAPSEVVAKVAELGDAEKKKLKLASPVTANKHLVNLVEYWEHLALHTKIP
                     PGFDNPFRGHLTARPRGRAARDEHPMWPADLDRALFTSTLYSGCQSIFRRGKPGKEIH
                     RDALFWVTMIGRITGAREDEICGRLVGDIDFIDTEIGRVAYLKIRNSKTPSSARDIPF
                     PELVLDAGFLEYRFYGRAPDEPIFPELIAQGAEPRRSAPFSGRFTEFRKKVRVYREGV
                     DFRAFRGNVETSLRNVEGVPAGWIDELIGHDSPIRRSEGARYTKGIFMVHLKRTIDRI
                     TIGIDPYSPQQDRPAVDFSHLRYNGPRGVQAPGAAEEIKRYVALAEREMRKKSTATQH
                     "
     gene            7772..7838
                     /locus_tag="VFC15_08115"
     tRNA            7772..7838
                     /locus_tag="VFC15_08115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7798..7800,aa:Arg,seq:tct)
     gene            complement(8672..8962)
                     /locus_tag="VFC15_08120"
     CDS             complement(8672..8962)
                     /locus_tag="VFC15_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZM10161.1"
                     /translation="MQTEILKRSIVIARHKTSVSLEDNFWNGLKEIADARDQSISGLV
                     TTIKMAHEQGNLSSAIRQFVLAYYRERPLIGAVAGRPGTSQPAREGASVASA"
     gene            complement(9484..>10192)
                     /locus_tag="VFC15_08125"
     CDS             complement(9484..>10192)
                     /locus_tag="VFC15_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZM10162.1"
                     /translation="DFGTGYSSLSYLMSFPFHKIKIDRCFIAALSANHESHVIVRAIT
                     DLARSLKLQVTAEGVETEQQLQQVRLLGCTEMQGYLLSPARPAAEILQFFTPCAEGAD
                     NLIAVNASAERKNGKMCGIRPDLVCSHRLTGRECDVLIGMMAGETSKESARHLNISPR
                     TIELHRGRIKNKFDVKNPADLVRMMLTTGCSPSRPNSAFSYSQSPEIKVAVSPLGGSV
                     EDTESDPSTYRRARAVT"
BASE COUNT         2296 a         2906 c         2909 g         2081 t
ORIGIN      
        1 acccatccct caagcgcgtt gcaacgtacc ggtcgtcagg cggtgaaaag ccgaaggcaa
       61 tcctgtgcgc ttcgtcagag gagatctgcg gccgcatcaa tcggactcaa caaccgcact
      121 ctcacgaata ggacgggcta agcaaatccc ttgccggcgc ggtaataggc gcgggcatct
      181 tggctttctc agcggtgagc gcttcggcag gtattgcctg tgtaggcctc gtttgctggc
      241 acgttcaaga cacctacgaa tatccatcag agtcccgcgt ggtcgttcat gaggacacct
      301 ggaaatgggg cccgagtgaa aagtacagtt tccgcgagca cgagggccgc ggttattgga
      361 agggcgacag ttggacggac tggaaagacg gcgccccaag tcgttagggt atcgttccga
      421 ggccgcctca gttggcggcc tctttcgtta tttcgattat ttcatcagcg ccgtacgcca
      481 agtcgaatgc catcgcgagc atcttcatcc aggttacgcg ctcatcggtg ggccaagggc
      541 ctttttccgg cagtttttga atgactgctt gaatgagatg gtcgcttgga ccaccaccac
      601 cgctaccacc gccgccgttc tcctcgcgta gaggaacatg ccctttatcg cggagagtgg
      661 cttagtcaga ggcagcgggg caacagatgg ggtaatcggc cggccggcgt gttgatcgct
      721 ggcgaatgtg ggtgctgcgc ttcaggctga tggtggaacg aggtccgcat tcgcggcctc
      781 aagcatcaga aggagaagcg tgagccatgg accattttgc tggactagac gtttcagtca
      841 agtttggcac cgggctttgg ccgacctgca gcgcatgaaa ctttgctact ctgcggccgc
      901 gtcgtcggca ccgaggtcgg tatttccttg gtgcgattaa gcccgaagtc aagttggacc
      961 cggggttcct tgagcacctc agactgtcgt cacgggccgt catcggcccc ggagcacgct
     1021 tcctagagtt cttatttacg ggaccccacg ccgacgacgc aacccaggag aaagtgatgg
     1081 aactgttgca tcgacgatgt tgcggtctcg acgttcacaa agagacggtg gtggcctgcc
     1141 tgcgccttgt atccaatggc aaggtcacga ctgaagttcg taccttccag acgaccacgg
     1201 cggaccttct gcacttgtcg gagtggttgg cggcaaacga ttgcatgcat gtggcgatgg
     1261 aggcgaccgg cgtctactgg aagccggtgt ggcacatctt ggatgatggc gagttcgagt
     1321 tggtgttggc aaacgccgcg cacgtgaaga acgtgccggg ccgcaagacc gacgtcaacg
     1381 atgcgatgtg gctggccgaa ctgttggcgc acggtctgat ccgggcgagc ttcgtgccgg
     1441 acacacagac gcaggagatg cgcaacctga tgcgcacgcg taagcaattg gttcgtgagc
     1501 agtcgagcca cgtgctgcgg gtgcaaaaga cactcgaaga cgccaacatc aaattggact
     1561 cagtgctcac cgatttgatg ggcaagagcg gtcgcgcaat gatcgaggca ctgattgccg
     1621 gcgaaaccaa ccccgccaaa ctggccagcc ttgccgatcg gagggcaaag gcgtcacatg
     1681 aagagttccg cgaggcgctt cgcggccgcg tgacgaagcc ccatcgcttc ctgctgcgcc
     1741 ttcatctcaa tcagatcgat gctctcgatg cagctatcgc cacgctcgac gcgcaagtgg
     1801 aggcgaacct tgggcccttt cgcaccgcag tcgacctcat aatatcgatc ccgggcatca
     1861 aaaacctcgg cgctcacgtc atcgtctccg agatcggcat cgacatgagc cggttccctt
     1921 cgcacgcaca tctcatatcg tgggctggca tatgtccgcg caacgacgag agtgccggca
     1981 agcgtcgatc caatcgcctg cgcaagggtg ctccctggct caaaaccacg ctggtgcagt
     2041 gcgcctgggc agcggtaaaa aagaaaaata gctacctgca ggcccaattc taccgcatca
     2101 aagcccggcg cggccccaag aaggcgatca tggccatcgc agcctccatc cttaccgcca
     2161 tctaccacat gctcaaggac gggacgatgt acaaggacct cggctgcaat cacttcgatc
     2221 gccgttccac cgaccagcaa aaacaacgct tggttaaacg cctagccgac cttggctacg
     2281 ctgtggagat caagccgctc gccgcctaga gagccgtgtt tgtggcaatg ccagcaaggg
     2341 gagtttgtgg cacggtccgt gtttgttagt ttcctcctag gagaccagcg tctgcattgt
     2401 ggatgacacg ggcaggatcg ttcgggaagt aaaggtggca agcgaacctg aagctctgct
     2461 gccggtgctg aagaatcccg cctaccgctt caagcggatc ggactggaag ccgggccatt
     2521 atcgcaatgg ctgttcagtg cactcgcgga agcaggcttg ccggtgatct gtgtcgagac
     2581 gcggcacatg cgggcggtgt taaaggcgca gatcaacaag accgaccgca acgatgcgcg
     2641 cggcatggcc cagatgatgc gtgcgggact ttatcgccca gtacatgtga agacgctacg
     2701 cagtcagaaa ctacgaatgt tgctgaccca tcgcaaactg ctgcagtcca aggccatcgc
     2761 catcgataac gacctgcgcg ggactttgcg taacttcggc ctcaaggtgg gcgtggtggg
     2821 aacggtgaag ttcgaggcgc gcatcaggga gcttgtcgag aacctgcccg atctagccgg
     2881 tctggtcgaa ccgctgctca aggtacggcg ggtactgcgc gagcagttca gcatcctgca
     2941 ccgccgtttg ctggccatcg tgcgagacga cgaggtgtgc cggcgcctga tgacggtgcc
     3001 tggtgtcggc cctgtggtag cgctgaccta tcgtgctacc gtcgacgtac ccgcacgctt
     3061 ccgaaactcc aaggcagtcg gggcggtgtt tggactgacg ccttccaggt atcaatcagg
     3121 cgagatcaac cgagccggcg ctatatcaaa gtgcggggac gagatgatgc gggtgatgct
     3181 ctacgaagcg gcccaggtaa tgctgacccg caccaacaag tggtcctggc ttaaggcctg
     3241 ggcgatgcag atcgccaggc gccgcggcat gaaaaaggca atcgtcgcgc tggcgcgccg
     3301 attggccgtg atcatgcacc gcatatgggt tgatggcacc gagttccgct ggacccggga
     3361 ggccgcagtg gcatgaaagc ctcacaagat caaatgcgat aggagaaaac ccgagctcca
     3421 ccgagcggtg gaatgatgtc cctcgcggga cgatggatga gatgagttcg catgtccgct
     3481 tgacctatcg tcaatctgac ggcaaggccg cgtaacagat tgttccgcct cgtcctcgaa
     3541 tcccataatg gaagggcctt ggtgccgatt ccgaagagaa gcacgagccc gtgagcgaca
     3601 tcgacactgc agtggtggat agtctgaaag gtagtgattg atggcttgaa agtgcttgac
     3661 cccgaacggc cgattagaga agcggacaaa ccattcggcg cgagtgccga cttttgtcgg
     3721 ctgtgccccc caatagcgga cattcggcgg gcgcggttgg attgtccgct atgtgccaaa
     3781 cgctgccatc agccaagcct tactcaatga tctcgtcgga gtgcgcaagc agcgtcggca
     3841 gcattaagcg ccttcatcaa gacggccgat tcagggaatc tcgccactaa ttgtctcccg
     3901 atctggtcaa attcgagcga cggtagcggt cgaagttctt cccggaagcc ggcgctttca
     3961 tcggccaaat aataactctt aaacggcatt ccgtgccccg gtgtatgagc atcataacgg
     4021 gtaagcttct ccatccgctc ttcgcgagaa tgaacgaccc aatcaaggtg gatcaagaac
     4081 gattcgagcg gagcattcag gccgcgtggg tattcgaaat caaatcccgg cgaatgaccc
     4141 cgtccgtgaa acgtcacttt atccggccgg tacagcctcc actggcggtg tgtgtgaggg
     4201 tcgtgtacca aattttgata aaggtcaccg ctgcaattga aaatgcactg atacctgttg
     4261 aagccaacga catgatactt cttgcacgaa atgacaccgc gaacaaattc catcatgcga
     4321 attgaaggca gttcgtcgtc atcgatccgc aatatccaac gagttctgca cagcctggac
     4381 atgccttcga tcatttcacc gacacgcgtt gccgggttct tgaacgttat gacttcgtga
     4441 gcatatcgcg ccgctatagc agccgtttca tctgtcgtct tgctgtcgac aaacacggtg
     4501 actgggatat tcatatcgcg atagtaggac aggatgatgt cgatatagcg tgcggaatta
     4561 atagttggca tgatgatctt aataaccccg gctgataact gccgatgcca aaactttgtt
     4621 tggggctgcc gtcgcaaccg ctcgaaaaga cctattagca attttcgtgc gccaaaattc
     4681 actgatgagg accgcgccat gtgcaattct aaagcctagc aattctgcga ccgaccagcg
     4741 agtgagaaat ggcacacaac cggcctcaaa cgctgattag gcctaattgt cgctgatggg
     4801 tcaatcgcga ccgcgcgaac tcgcacgaaa atccaccaat gtccgctatt gcccgaaagc
     4861 gacacgattt cagcgctcct cgggactgca aggcaagctc gtcctcctct gtaaccgagg
     4921 agaacgacca tgcaagacaa tgccaatttc gagctgccga cactcaagcg gataccgtgg
     4981 aacaaaggga aactgacagg cgccaagcca ccgttacggc aaaaacacgt ctgggcgatc
     5041 cgtacaaagc tacaaatcga ccaacgaccc cgcgatctgg cgatgttcaa tctggccatc
     5101 gacagcaaat tgcgaggctg cgatgtcgtc gctatcaagg tcgaggacgt tgctccgaat
     5161 ggctatgctg tcgatcgggc gaccgtgcgc cagaaaaaga ccggtcgtcc cgtccggttt
     5221 gaattgacgg aacagacccg ccaggcggtg gacgattaca tcagaacggc cggcaagaaa
     5281 cctggcgggt tcctgttcac agcccgccgc gggcaggaag accgtatgac gacgcggcag
     5341 tatgctcggc tgctatctga gtggatcgcc ggcatcggat tagacccaca cctgttcggc
     5401 acccattccc tgagacgaac aaaggcaacg ctgatctatc gccggaccgg caacctgcga
     5461 gccgtacagc tattgctcgg ccacaccaag attgagagca ccgttaggta tctcggcatt
     5521 gaggtcgatg acgccctttc catcgcggaa caggtcgacc tctgatctac ccgggcggag
     5581 cgggtccgct ccgcccgaac accctgcccc cgaagcgaac cgtgctgcct aggcggcgaa
     5641 gcgcaactcc cggatatcct ccaaaatctg ccgaaaatcg ggttccggga cgcggcacat
     5701 agttcgatat gtatccagga agggcagcca gactcgaaaa atccttcgca gatctaggca
     5761 cttccaaccc gcagccttcg aaactgccgc aatggtccac aacgatggtc cacagcgaga
     5821 gcggcgatgg cgttcggtcg gtacctgcgg cggcgaggtt gtacatggtt ttttcgctgg
     5881 cgctggccgg cagcgcttgc tgcttgccgg ttcgtcggcg agcttaatgt gagcctgcgg
     5941 actggtgact accgcctcgc gctacatcgc gcccgcgtcc tcaggcttgg cctggagacg
     6001 ctcatgaccc gtttcacgcc gtcgacatcg aaatcggagg ccgaggctat cgtccggcaa
     6061 tggatcgacg cctgcgtgtg gcgccaggaa gctcaccttg ccgaaaccgg cggaatagcc
     6121 ttccttgaga cggacgagat cgaaaagctc ggccgggagg atgccgccga gcttgatgcg
     6181 ctcttgtggt tcgctgatcg caagcacgcc gatgacgaga aggcacaaat cgcccgggcg
     6241 cttggccccg tcggcccggg gctcgaagct ttcggccagg tcatcgacgc ggccggccgg
     6301 acgatgggcg tcccggtagg ccggcaaacc gtcgacgggc gtctctcagc acgaacgttc
     6361 ctgcgcggct accaaagcct gcttgacgag ctgcgggaga ccgtagccgc catcccccgc
     6421 caggtacagg cggtggtggc caaaccggtg ctgccggatt ttgacttcct cacttattgg
     6481 gatgaatttt ccgccaccaa gcagagcaac aaggactgga aggccgacac cgcggccggc
     6541 gccgacgcaa cgccaagatt gttcagatcg ctcgtcggcg acctgcctat aaacaaaatc
     6601 gacggcggcg tcgcagggga atttcgcctc gaatatctca agctgccgtt cgactactac
     6661 cacaacaaga gatggaagaa attggcgccg agcgaggtgg tcgcgaaggt cgccgagctc
     6721 ggcgacgccg agaagaagaa gttgaagctg gccagccctg tcactgccaa taagcacttg
     6781 gtgaatttgg tcgagtactg ggagcacctg gctctccata caaaaatccc cccaggtttc
     6841 gacaatccat tccgcggcca tctcacggct cgcccgcgcg gccgcgccgc acgcgacgaa
     6901 catccgatgt ggccggccga tctcgacagg gcgcttttca cctcgaccct ttacagcggc
     6961 tgccagtcga ttttccggcg cggcaaacca ggcaaggaaa tccatcgcga cgctttattc
     7021 tgggtaacga tgatcggccg catcacgggc gcacgcgaag acgaaatctg cggccggctg
     7081 gtcggcgata tcgattttat cgatacggaa atcggccgcg tggcgtacct gaaaattcgc
     7141 aattcgaaga cgccctcctc ggcgcgcgac atccccttcc ccgaattggt cttggacgcc
     7201 gggttcctcg aataccgctt ctacggccgc gcgccggatg aaccgatttt tccagaactt
     7261 atcgcgcagg gcgccgagcc gcgccgcagc gcgccattca gcggacgatt taccgagttc
     7321 cgcaagaagg tccgcgtata tcgggaaggg gtcgattttc gggcgttccg aggaaatgtg
     7381 gaaacttcgc ttcgcaacgt tgagggcgtc cccgccggtt ggatcgacga attgatcggg
     7441 cacgattccc cgatccgtcg ttccgaaggg gcgcgctata ccaaggggat attcatggtc
     7501 catctcaagc gaaccatcga tcggatcaca atcggcatcg atccctatag tccgcaacaa
     7561 gaccgccctg cggtggattt ctcgcatctg cgctacaacg ggccccgagg cgtgcaggcc
     7621 cccggcgccg ccgaagaaat taagcgctac gttgcactgg cggagcgcga aatgcgaaaa
     7681 aaaagcaccg cgacgcagca ctaatcggcc ctgagtaccc aatgctgctg gatctcaccc
     7741 tgccgatgga tagccactcg aaacatttgc tcgagagtgt tcttagactc tcagccttct
     7801 aagctgaagg tcgctggttc gaatccagcc ggggtccaat gaattcagct gtttgtccgc
     7861 tttgcgccgt tagcgcccat tcaaggcacg agccggaaat gacgctaata ccaaatcgcg
     7921 ttacccgcga cccatctaag gcgttgcggc gggtttgtga cgtccgtcca ggtgtttgat
     7981 ttgactcacg tttacgatgc ggccatcccc gcacaaagtc gggcaacatg agtcaagatt
     8041 tctgagtttt ggtataagtg ccaataaccg acatcgcgcg cttggcttga aatgaaaaaa
     8101 agccgccaac tgaggcggcc tcagtcaaag agacttgggc gcggccgagc gacttgatta
     8161 tgcgggctcg ccttctgctc aattgagcgc atgtcgccgc atgcgaaaca tcgaaagatg
     8221 tgcaattcag taaatgacgc gacccttggc tcaatgaaca caagtgccat cgatatcccg
     8281 caaatcgggc atggcagtgc agggtgaact tgcggagcgc ccatattgtt gggtaatcgg
     8341 aacttgaaga ttcgttcaac tgcggatgtt tgcctaatgt gtgggccatc aggccgccag
     8401 ccgatgtggc ctagtcgacg cggatcccaa tacgccgcgc ggaacgccac gcaacgcggc
     8461 acggtcgctt cacgccgtct ttgctgataa ccaagataaa attgtcggat atgccgatcg
     8521 ggctttcgat ttcgagcaat gcacccgttt cggatacgtt gcacacagtg cagtcgattc
     8581 cagcggctag ttcaaatcag atcgtcatgg ctctcaggat tcactggcgt gggcatcttc
     8641 tattttcatt catggcaaca aggcccggcg gttaagccga cgcaacactc gcaccttctc
     8701 ttgcgggttg agaagtgcca ggtcgacccg ccaccgcgcc aatcaacggt cgctcacgat
     8761 agtaagcaag aacgaattgg cgaatagctg aagacagatt gccttgctcg tgtgccattt
     8821 ttatcgtggt cacaaggcca gagatcgact ggtctcgagc gtccgcgatt tccttcagtc
     8881 cgttccagaa attatcctca agactcacac tagtcttgtg acgagcaatc acgattgaac
     8941 gcttgagaat ttccgtttgc ataagcgatc ctcctatccg tccattagct gcaaaaacgg
     9001 tataactgaa tacctttttc atatgtacaa acacatatga acttgttttc gggggcatag
     9061 gtaaaaatac taatgcgctt accctaaaga tgaccgcctg atctgctcgc gctaatattg
     9121 ccaactaatc gtaccataaa cttgggcagt caggcctcaa ccaaacgatt acggccgccg
     9181 gctttcgcag cataaagcgc ccgatcggcg gcacaaataa tatcctgtgc cgtatcacca
     9241 ataccgtcgg agaatgctac cctgaggctt gctgtgattc ttttgatctc agtaccaaga
     9301 ctgaaaggta aagtggcgat cgtctgtgtt cctcgtccct aagaggacat ctagagttat
     9361 agagttactt tgcgaacgag tgagagaagt cgagagatcg ttcgttcctg accagtaacc
     9421 cacggctatc gtgcgcatgg cgaccggatc cgcccacaaa ctctatgacc ccgtgataga
     9481 cagtcacgtg acagcgcgag cgcgccgata agtacttgga tcagattcag tatcttcaac
     9541 ggaaccgcca agaggactga cggcgacttt gatctcaggc gattgagaat agctgaaagc
     9601 agaattaggg cgcgaaggtg aacagcccgt cgttagcatc attcgaacga gatcggctgg
     9661 gttcttgacg tcaaacttat tcttgatgcg tccgcgatga agttcgatgg ttcgcggact
     9721 tatgttgaga tggcgagcag attccttgct ggtctcacca gccatcatac cgatcagaac
     9781 gtcacattca cgccccgtca gccgatggct gcagacaagg tccggtctga tcccacacat
     9841 ttttccattt tttcgttccg ccgacgcgtt gaccgcgatc aaattgtccg cgccttcagc
     9901 gcagggggtg aaaaattgca atatctccgc ggcgggtcgc gcagggctaa gaagatagcc
     9961 ctgcatttcc gtgcagccga gtagtctgac ttgctgcaat tgctgctctg tttcaacgcc
    10021 ctcggcggtg acctgcagtt tgagacttcg agcaagatcg gtgatggcgc gaacaatgac
    10081 gtgagattca tggttggccg atagagcggc aataaagcat cgatcgatct tgatcttatg
    10141 gaacggaaaa ctcatcagat agctgagcga tgaatagccg gtgccgaaat ca
//
LOCUS       DASRKK010000368         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1006309, whole genome shotgun sequence.
ACCESSION   DASRKK010000368 DASRKK010000000
VERSION     DASRKK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006309"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFC15_08130"
     CDS             complement(<1..196)
                     /locus_tag="VFC15_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZM10163.1"
                     /translation="MDRAPSATVDSFSFDVVVVGAGPTGLACGIDAKRAGFTAANIDK
                     GCLVNSLYNYPSNMTFFTTPE"
     gene            complement(610..2058)
                     /locus_tag="VFC15_08135"
     CDS             complement(610..2058)
                     /locus_tag="VFC15_08135"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM10164.1"
                     /translation="MFQSNWFLKLGASGSCAIAALLLAVSLQAQVDDTTRQLSRDIFK
                     QLIEINTTDSVGNVSTAAEAMAQRLRDAGFPDSDIQVLGPNDRKKNLVARLHGTGAKR
                     PVLLIGHLDVVEARRQDWTTDPFQFVEKDGYFYGRGTQDMKSADAIDVATLIRFKQEG
                     YKPDRDIVLALTADEEGGKSNGVDWLLRNHRDLIDAEFVLNADGGGVDTVKGKPLDVS
                     VDAAEKLYGDYQLEVTNSGGHSSLPVPDNAIYRLADGLTRLQAYLFPFELNAVTHTYF
                     QRMATVETGQTAADMRAIVKSPPEQVAIRRLSANPIYNATMRTTCVATRLDAGHANNA
                     LPQRATANVNCRILPGHSREEIRQTLVKILADPKITARYVSNDGTVLDKAPEQTSLPP
                     VVLRPEVMKPLEEVAASMWPGLPVVPSMSTGASDGVYTNAAGMPTYGISGIAIDTNDV
                     RAHGKDERLRVESFYQGLDFYYRYLKVLTSEP"
     gene            2702..>2956
                     /locus_tag="VFC15_08140"
     CDS             2702..>2956
                     /locus_tag="VFC15_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10165.1"
                     /translation="MLQDLRYGLRQLRKSPGFTLVAVLTLALGIGANTAVFSVVDAVL
                     LRALPYRQPERLMLVSETESVSPNEELGVAAQEYLDYREQN"
BASE COUNT          564 a          875 c          901 g          616 t
ORIGIN      
        1 attcaggcgt ggtgaagaac gtcatgttgc tgggatagtt gtagagcgag ttcaccaggc
       61 agcctttgtc aatgttggcc gccgtgaagc cggcgcgctt cgcgtcaatg ccgcaggcca
      121 ggccggtcgg gccggcgccg accaccacca cgtcaaagga aaaactatcc accgtcgctg
      181 aaggtgcgcg gtccatatag aaagcatacc ctgtcccgcg cgttacaggt gtccctggag
      241 cagtttcacc tttgatctat tggcaacggg tggagcacgc cttcaggcgt gcattgggag
      301 cggcaatttc tgatcggcgc tttagcgcct gaggtaaagg ggttcaatta tctcagcggc
      361 tcaaacaggc tgcggaaaaa gtcggaaaag cagatccctc gcgggctgaa gcccgctcgt
      421 gatgacaaaa aacaagcgca cctaaaggtg cgccccttca aaacactggc tgaaaccggc
      481 tttttcagca gcctctcaag tcggtttccc gagcgatcgc cgaatgcagc gctgaagcgc
      541 tgctccaccc tatacaccaa aagtgaaact tctctgggga ggcccaccgt agttccatct
      601 ggcccgaggc tacggttccg acgtgagcac cttcaagtag cggtagtaga aatccaatcc
      661 ttgatagaac gactcgaccc gcaggcgttc gtccttgccg tgggcgcgca cgtcattggt
      721 gtcaatcgcg atcccggaga ttccgtaggt tggcatccct gctgcgttgg tatacacgcc
      781 gtcggacgcc ccagttgaca tggagggcac aaccggcagg ccgggccaca tgctggcggc
      841 gacttcctcc agcggcttca tcacctcggg acgcagcacg acgggcggca gcgacgtctg
      901 ctcaggggct ttgtccaaca ccgtcccatc gttgctcacg tagcgcgccg taatcttcgg
      961 atcggccaga atcttgacca gcgtctggcg tatctcctcg cgggaatgtc ccgggaggat
     1021 gcggcagttg acgttggccg tcgcccgctg cgggagcgcg ttgttggcgt gccccgcgtc
     1081 gagccgcgtg gccacgcagg tggtgcgcat ggtcgcgttg tagatgggat tagctgagag
     1141 ccggcggatt gccacctgct ccggcggact cttcacgatg gccctcatgt ctgccgcggt
     1201 ctggcccgtc tcgaccgtgg ccatccgctg gaagtaggta tgggtcactg catttaactc
     1261 gaagggaaac aggtaagcct gcaaacgcgt gaggccgtcg gccaagcgat agatcgcatt
     1321 gtcaggaacc ggcagcgagc tgtggccgcc ggagttggtc acctcgagct ggtaatcgcc
     1381 atagagtttt tcggcggcgt cgacggacac atcaagcggc ttgcccttga ctgtatcaac
     1441 gccgccgccg tcggcgttca gtacgaactc cgcatcgatc aagtcgcgat ggttgcgcag
     1501 cagccagtcg acgccgttcg acttgccgcc ctcttcgtca gcggtcagcg cgaggacgat
     1561 gtcgcggtcg ggcttgtagc cttcctgctt gaatcggatc agcgtagcca catcgatggc
     1621 gtcggccgat ttcatgtcct gcgttccgcg accgtagaag tagccgtctt tttcgacaaa
     1681 ctggaaagga tcggtggtcc agtcctgtcg ccgcgcttcc acgacgtcga gatggccaat
     1741 cagcaggact ggccgctttg cgcctgtgcc gtggagccgc gccaccaggt tcttcttgcg
     1801 atcgttcggc cccagcacct ggatgtcgct gtccgggaaa ccggcatcgc ggagccgctg
     1861 ggccatggct tcggccgcgg tggaaacgtt gcctaccgag tcggtggtat tgatctcgat
     1921 aagctgcttg aaaatgtcgc gggagagctg gcgggtggtg tcgtcaactt gcgcttggag
     1981 cgacacggcc aacaacaacg ctgcaatcgc gcaggagccc gacgccccga gtttcaaaaa
     2041 ccaatttgat tggaacattt gccccactct gaatgagtcg agaaagagat taccaaactg
     2101 gtgactgccc ggtcattaag ggcggttcca gagtagcttg tggctcgggt agtgcgggcc
     2161 tttagagcgg tttcagagtt agcgtagacc tgaccgggta gtgcggacct tcaggtccgc
     2221 gttaatagcc ttttagaaga accgggcttt agccactggg gtattcggtc tggcggcctt
     2281 taggctgatt caaacaccga acctcacggc ctaagggccc ttccatccgg agccttatga
     2341 cgcggcgctg aagcgccgca ctacccgagc gtcggtcttg ctccgggttg gtattgctgc
     2401 ggaggcgaaa cttccgcagt tcacactaac tctgaaactg ctttagaggt ctccatcaga
     2461 tcatccaccc ccagcttcgg cagggagttc gcgtttttgt ggggctggcg cccggcccta
     2521 ctgaacccgt ctttctgtcc ggttgcgaac atcggcgttt cgctggcgga cattccgagt
     2581 accacgccat ccctaagtta ctgaaaacta ggctcccgac atggccagca ccttgcattg
     2641 tcattaccga accatccctg cgaatcaggg cggcacggaa agtggaggcg cggagaacga
     2701 aatgcttcaa gaccttcggt acggactgcg gcagctgcgc aagtcgcccg ggttcactct
     2761 ggtcgcggtg ctcacgctgg cgctcggcat tggggccaat accgccgtat tcagtgtcgt
     2821 ggacgcagtg ctgctgcgtg cgctccctta tcgccagccc gagcgcctca tgctggtttc
     2881 cgaaaccgag tcggtttctc cgaacgaaga gcttggcgtg gcggcccagg agtacctcga
     2941 ttatcgggag cagaac
//
LOCUS       DASRKK010000369         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1201518, whole genome shotgun sequence.
ACCESSION   DASRKK010000369 DASRKK010000000
VERSION     DASRKK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201518"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="rpsM"
                     /locus_tag="VFC15_08145"
     CDS             complement(<1..269)
                     /gene="rpsM"
                     /locus_tag="VFC15_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZM10166.1"
                     /translation="MARIAGVDLPRNKHVSIALTYIFGIGDSRSKSILASASVEGNKK
                     VSDLNEDEVNRIRQVIEQEGNVEGDLRKDTSMHIKRLIEIGSYRG"
     gene            complement(324..437)
                     /gene="rpmJ"
                     /locus_tag="VFC15_08150"
     CDS             complement(324..437)
                     /gene="rpmJ"
                     /locus_tag="VFC15_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZM10167.1"
                     /translation="MKVRASVKKICDKCKVIRRHGVVRVICENAKHKQRQG"
     gene            complement(459..677)
                     /gene="infA"
                     /locus_tag="VFC15_08155"
     CDS             complement(459..677)
                     /gene="infA"
                     /locus_tag="VFC15_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZM10168.1"
                     /translation="MSKEDAIEVMAVVLEPLPNAMFKVELENKHQVLAHISGKMRKNF
                     IKILPGDRVAVELSPYDLTRGRIVYRYK"
     gene            complement(709..1461)
                     /gene="map"
                     /locus_tag="VFC15_08160"
     CDS             complement(709..1461)
                     /gene="map"
                     /locus_tag="VFC15_08160"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZM10169.1"
                     /translation="MSIVCKSVSEIEKMRRSGHIVRQVLDSVKEVVAPGVSTMDLERV
                     AERKIKELGAKPAFKGYYDYPCVLCTSINDEIVHGIPSERRVLKTGDIVSIDTGVVLD
                     GYYGDAAITVPVNGGLSPELQTLLDVTRESLYRGIEAARVGNTIGDVGSAVQRLVESN
                     GFSVVREFVGHGIGTKLHEDPQVPNYGAPGSGPRLRDGMVLAIEPMVNVGRSETRLLD
                     DKWTAVTQDGSWSAHFEHCVAVTKNGPVILTE"
     gene            complement(1445..>2075)
                     /locus_tag="VFC15_08165"
     CDS             complement(1445..>2075)
                     /locus_tag="VFC15_08165"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HZM10170.1"
                     /translation="LISSAYGIPQISTGDLLRDNVARETELGSKVKALLGSGALVSDD
                     LVCDMVAERLRNPDCERGYILDGFPRTVVQAEWLDRYLKSRQTGNGTTPSTGMKPVVI
                     RLVVEYNRLLQRITGRRSCPTCGRIYNVYFQPPKVEGICDVDGGRFVVRPDDTEEVVV
                     ERLKAYEKLTLPLVDYYHTKGRLVEINGDQSVEVVAAQAFNAIENVNRL"
BASE COUNT          420 a          676 c          503 g          476 t
ORIGIN      
        1 tacccacgat acgatccgat ttcgatcagc cgcttgatgt gcatcgaggt gtctttgcgg
       61 agatcgcctt ccacattgcc ttcctgctcg atgacctggc ggatgcggtt gacctcatcc
      121 tcgttgagat cgctgacctt cttgttgcct tccacgctcg ccgaggcgag aatgctcttg
      181 gaacgggaat ccccgattcc gaagatatag gtcaatgcga tgctcacatg cttgttgcgc
      241 ggaagatcca cgcctgcgat acgtgccata gttgctccag aacccttcag ctagttacct
      301 gttacgggta actaggaact tatttatccc tgccgctgct tgtgcttggc gttctcgcag
      361 atcacgcgga ccactccgtg acggcggatg accttgcact tgtcgcaaat tttcttgacc
      421 gatgcccgga ccttcatgat gacctaaatt cccttcggct acttgtaacg ataaacaatg
      481 cgtccacggg ttaagtcgta gggcgaaagc tccacggcca cccggtcgcc cggaagaatc
      541 ttgatgaagt tcttgcgcat cttgcccgat atatgggcca ggacctggtg cttgttctcc
      601 agttccacct tgaacatggc attgggcagt ggttccagca ccaccgccat cacttcaatc
      661 gcgtcttcct tactcattca gctcctgaaa ctaaactttt tcgtcactct actcggttaa
      721 gatcactggc ccattcttcg tcaccgccac gcagtgttcg aagtgggcgc tccaactgcc
      781 gtcttgcgtg acagccgtcc acttgtcatc cagcaaccgc gtttccgaac ggcccacatt
      841 gaccatcggc tcgatcgcca gcaccatgcc atcgcgcagt ctcggaccgc ttcccggagc
      901 tccgtaattc gggacctgcg ggtcttcgtg cagcttggtg ccgatcccgt ggcctacaaa
      961 ctcgcgaacc acgctgaaac cgttggactc aaccagcctc tgcaccgccg agcctacatc
     1021 tccgatcgtg ttgccgactc gcgccgcttc gattccccga tacaacgact cgcgggtcac
     1081 atctagcagc gtctgcagtt cgggactcag accaccattc accgggaccg tgatcgccgc
     1141 atcgccgtaa tacccgtcca gcaccacgcc cgtatcgatg gagacgatgt cgccggtctt
     1201 caacaccctg cgttccgacg ggatgccatg cacaatctcg tcgttgatcg acgtacaaag
     1261 aacgcaaggg tagtcgtaat aacccttgaa cgccggcttg gcgcccagtt ccttgatctt
     1321 ccgttccgcc acccgttcca agtccatcgt gctgacgccg ggagcgacta cttctttgac
     1381 gctatccagc acctggcgga cgatgtgccc gctacggcgc atcttctcga tctcggaaac
     1441 cgacttacaa acgattgaca ttctctatcg cgttaaatgc ctgcgctgca accacttcca
     1501 ccgactggtc gccattgatc tcaaccaacc gtcccttggt gtgatagtaa tccaccaggg
     1561 gcagtgtcag cttctcgtag gctttcaggc gttcgacaac aacttcctcg gtgtcgtcgg
     1621 gacgcacaac aaatcgtcct ccgtcgacat cacagatgcc ttcaactttg ggaggctgga
     1681 aatagacgtt gtaaatacgc ccacaggtgg gacaagaacg acgcccggtg attcgctgca
     1741 gtagacgatt atactccacc accaagcgga tcacaaccgg cttcatgcca gtcgagggtg
     1801 tcgttccgtt gcccgtttgc ctgcttttca aatatctgtc cagccactcg gcctgcacca
     1861 cggtccgtgg aaagccgtcc aaaatgtacc cgcgctcaca atccggattc cgcagccgct
     1921 ccgccaccat gtcgcagacc aggtcatcgg aaaccagcgc gccgctgccc agcagcgcct
     1981 tgaccttcga acccaactcc gtctcgcgag ccacgttgtc tcgcaacagg tcgccagtcg
     2041 aaatctgcgg gatgccgtag gcgctggaaa tcagc
//
LOCUS       DASRKK010000370         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_810393, whole genome shotgun sequence.
ACCESSION   DASRKK010000370 DASRKK010000000
VERSION     DASRKK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810393"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFC15_08170"
     CDS             <1..315
                     /locus_tag="VFC15_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10171.1"
                     /translation="EKPLILNVGPHLLYMQAHIPGSEYIGAGSDSQGIESLRRRVKPL
                     PRKTFIVLYCGCCPWGHCPNVRPAYNELHKAGFTNVKVLYIADNFGADWVYKGYPTIK
                     GQ"
     gene            321..848
                     /locus_tag="VFC15_08175"
     CDS             321..848
                     /locus_tag="VFC15_08175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM10172.1"
                     /translation="MRKAVLIVVAILLVFAIYEAGRRNSAHSKPGRPSAAVHSVAPDF
                     SLRDLNHQTLELANYRGKVVLLDFWATWCTPCRDEIPRFVEFQNKSRERGLQVIGISM
                     DDDAKPVREFYQQFKMNYPVAFGNEKVAEAYGGVLGLPITFLIGRDGRVAAKHIGEVD
                     MSVLEQEIETLLQAK"
     gene            874..1401
                     /locus_tag="VFC15_08180"
     CDS             874..1401
                     /locus_tag="VFC15_08180"
                     /inference="COORDINATES: protein motif:HMM:NF039354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HZM10173.1"
                     /translation="MSNNSKTAFDSMGREDLLRALEMFAKNWLAHDGCWFLAAEERFG
                     MEIAIELDTRSWEGFAAAEARRIMTVFHVPQQGGLDALEKALALRMYSVINAQRVEWS
                     EDRKRLRFFMDVCRVQETRRRKGLPDFPCKSVGTVEFETFARTIDSRVRTTCLHCPPE
                     AAEGQYCGWEFSLEE"
     gene            complement(1695..>2478)
                     /locus_tag="VFC15_08185"
     CDS             complement(1695..>2478)
                     /locus_tag="VFC15_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM10174.1"
                     /translation="PNTKLVIVNHGSNVIGTVQPVREIGRICRERGLTFVLDTAQTAG
                     IVPINMQEMNIDVVAFTGHKALMGSMGIGGLCVRKHVVVKQVRSGGTGVRSIHPFHLD
                     EYPWRLEYGTPNMVGVASLWAGQDWLDENGIASVHAREMKLAQRLVDGFRRIEGVQLY
                     CCDSLENHLSTILMNLEGVDPGDIGVMLDVDHNIATRTGLHCAPLVHTQLGTVQRDGG
                     VRFSIGAFNTEQEVDAAIEGIADIAQWAHERAAKPRGALAHA"
BASE COUNT          498 a          722 c          735 g          523 t
ORIGIN      
        1 gagaagccgc tgatcctgaa cgttggtcct catctcctgt atatgcaggc gcatattccc
       61 ggttccgagt acataggggc cggttcggat tcgcagggaa tcgaatctct gcgcaggcgg
      121 gtaaagccgc tgccccgcaa aacgttcatc gttctttatt gcgggtgctg cccgtggggc
      181 cattgtccca acgtgcggcc cgcgtacaac gagttgcaca aagcgggctt cacaaacgtg
      241 aaggtactct acatcgcgga taacttcggg gccgactggg tgtacaaggg ctaccccacc
      301 atcaaagggc aatagcttcg atgagaaaag ccgtcctgat tgttgtcgcg atcctactcg
      361 tgttcgctat ctacgaagcg ggccggcgga attccgcaca ctccaagccc ggccgcccgt
      421 cggctgccgt tcattcggtc gcgcccgatt tctcgctccg cgatttgaac caccaaacgc
      481 tggaactcgc caactaccga ggtaaagtgg tcctgctcga cttctgggcg acgtggtgta
      541 cgccctgccg agatgaaatc ccccgctttg tggagttcca gaacaagtct cgcgagcggg
      601 gcttgcaagt gattggtatt tccatggacg atgatgcgaa gccggtccgc gagttctacc
      661 agcaattcaa gatgaactac cccgtcgcgt ttgggaatga aaaggttgct gaggcgtacg
      721 gaggcgtttt gggattgccg atcacatttc tgattggtcg cgacggccga gttgcggcaa
      781 agcatattgg cgaggtggac atgtcagttc tcgaacagga gatcgaaact ctgttgcagg
      841 cgaagtgagg ccatgggccg gggtgaaacg aacatgagca ataactctaa gacagctttc
      901 gattcgatgg gccgcgagga ccttttgcgc gcgctcgaga tgtttgccaa gaactggctg
      961 gcgcacgacg gatgctggtt cctggcagcc gaggagcgct tcggcatgga gatcgcaatc
     1021 gaactggaca cacgttcctg ggaaggattt gctgcggctg aggcacgccg catcatgacc
     1081 gtgtttcatg tcccgcagca gggcggtctc gacgcgctgg aaaaggcgct ggcattgcgc
     1141 atgtacagtg tcatcaatgc gcagcgggtg gaatggtcgg aggacagaaa acgcttgcgc
     1201 ttcttcatgg atgtttgcag ggtccaggaa acccggcggc gcaaggggct tccggatttt
     1261 ccctgtaaga gcgtaggcac ggtggaattc gaaacgtttg cccgaaccat cgactcccgt
     1321 gtccgcacga cctgcctgca ttgtcctccg gaagcggcgg agggccaata ctgcgggtgg
     1381 gaattcagcc tggaggaata gcgctttcag ccgttcctct gccaccaagc gcgtttgacg
     1441 ctcgtgctca gctgcgaaag catctgcttt tctgtctgtc atcctgagcg agcgcagcga
     1501 gtcgaaggac ccctcttgct gcaagatcgc tgtgtgcccg cgcggcacag caacaagtct
     1561 ggatcgctgg cggccaaagg ggtccctcga ctcctcgctg cgctcgtcgc tcaggatgac
     1621 gtccaaaaaa catatgtggg gtcatgaata atccagaagc gccgctctac ccttgtacct
     1681 aaggtgaaac cgctctaagc atgggctaga gctccacgcg gctttgccgc ccgctcgtgc
     1741 gcccactggg cgatgtcggc aatcccctca atggccgcat ccacttcttg ctctgtattg
     1801 aaggcgccga tcgagaagcg cacgccacca tctctctgaa cggtcccaag ttgggtgtgc
     1861 accagcggtg cgcaatgcag gcccgtgcga gttgcaatgt tgtggtccac gtcgagcatg
     1921 accccgatgt cgccgggatc gacgccctcc aggttcataa ggatggtcga gaggtggttt
     1981 tccaggctgt cgcagcagta caactggacg ccttcgattc gcctgaagcc atccaccagt
     2041 ctctgtgcca acttcatctc gcgcgcatgg acactcgcga tgccgttctc gtccaaccag
     2101 tcctgccccg cccacaacga cgccacaccg accatgttcg gagtgccgta ctcgagtcgc
     2161 cagggatact cgtcgaggtg gaacggatgg atggaccgaa cgccggtccc gccgctgcgc
     2221 acttgcttga ctactacatg cttccgaaca cagaggccgc cgatgcccat gctccccatg
     2281 agcgctttgt ggccggtgaa tgcgacgacg tcgatgttca tctcctgcat gttgatggga
     2341 acgatgccgg cggtctgagc agtatccaag acgaaggtca gaccgcgctc gcgacagatt
     2401 cttccgatct ccctgacagg ttgcaccgtg ccaatcacgt tcgaaccgtg gttgacgatc
     2461 accagcttgg tgttaggc
//
LOCUS       DASRKK010000371         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_908077, whole genome shotgun sequence.
ACCESSION   DASRKK010000371 DASRKK010000000
VERSION     DASRKK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908077"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..544)
                     /locus_tag="VFC15_08190"
     CDS             complement(119..544)
                     /locus_tag="VFC15_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10175.1"
                     /translation="MGEPHVISALSNKRAELAGIVSQLERQLGQQQTNLAHLDATMRL
                     FDPDIRPNKIRPRHQRARSVWFRPGECLRLIHDELRDAVQPMTTRELAERIMRVKAMP
                     MADDRQRELVQKTILGSLNRAKQTIARVEAAGIVSWRLA"
     gene            1006..2793
                     /locus_tag="VFC15_08195"
     CDS             1006..2793
                     /locus_tag="VFC15_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10176.1"
                     /translation="MGEQTSNEISVVETLAVDPTTVPEISPGGQRKNIPLAANSIQVN
                     GCKNPRCQNFCIPPSEYQQKRGRGNLSEDGYKVSGASSTGLICRHCDKSSRIKSNQAI
                     AEEYERQSAYLQPQARLCCPNPSCANHIPTNQVGDTGFRRFGKTHSGSARWQCKLCKK
                     TFSVGSSTRYHKKPHLNMQAFELITNKVSISRMTEILGISYTSVYGKIDFLYNQCLTF
                     SASRERLLSSLTYDRLYLSTDRQDYIVNWGDRKQRKTIQLTSIGTADRYSGYVFGLTP
                     NFDAAVNPEQLERDWAACGDGAKAPAMRQFARLWTLDDYDASVAKAANAGDRQDIATR
                     DDLDAPELLSVGQQLPTSGAQVHAEYIMHGHYWLLRSLFPKAEKLRFFIDRDAGLLHA
                     CMGAFGDRVKARTADVVIVDYLKAITVDERKKLFTAARIWFDGQKCLFPGLSDGEVRT
                     AIFAGEIRAARRLYDNNISGLWFDHRFPDMGEPSKRFKFVTDLADYDDEHVANLLLLA
                     TLWPIDTVFNRIRRRMAAFERPVASVSRARRLWHLYAPYEPAMVEKLLVIYRTWHNFV
                     WVSPKTDRTAAELLGLTSGKIRVQDIVYF"
BASE COUNT          719 a          928 c          939 g          698 t
ORIGIN      
        1 ggcgcgagga cttccgccgc gtcagcaacg gcgagcaggt gtatagcgtc gtcgggctgg
       61 cgatgcggtg caagccgtcg gtcgatttct gcggctactg gcaacgcgcg cgtgccgctt
      121 aggccagccg ccaactgacg atccccgcgg cctcgacgcg cgcgatcgtc tgcttggccc
      181 ggttgagcga accgaggatc gttttctgaa ccaactcacg ttggcggtca tcagccatcg
      241 gcatggcctt gacgcgcatg atccgctcgg cgagctcacg cgtcgtcatc ggctggaccg
      301 catcgcgcag ctcgtcgtgg atcagccgaa ggcactcacc aggacggaac cagacactac
      361 gcgcccgctg atgcctcggc cggatcttgt tcggccggat gtccggatcg aacaaccgca
      421 tcgtcgcatc gagatgcgcc agattggttt gctgctgccc aagctgtcgt tcaagttggc
      481 tcactattcc agccagttcc gctcgcttgt tgcttaatgc gctgatgaca tgcggttcgc
      541 ccattaccga ctgctcctgg aatactacag gccaggatag tagacgacat gttctccacc
      601 gcaaatatac tggtggtgga ggctgctaca taataacgca taatgccggt gatgcgcgca
      661 tcgcgaccag atcagagagc gctccgggga gaaaggccgt ggccggtcgg caagagtatc
      721 cgcgctgcac gctttgagcc gatgaatgtg cagtttgcca gccgccaggg cggcgcggac
      781 atggctaccc gatccccacc cggctgatgc ggagaaatcg gctccgtccg gggtcccgtg
      841 acctctcctt ctgggtcttg catggtctca gtcccaccta tggcgatggc gggaggaccc
      901 ccccagcccc tacacaaagg tgttgacgcc tgataccgcc tccgcaatac ttcgccgccc
      961 gttcagccgg gtatcggatg gcggtcagat ggggcgattc gaggaatggg agaacagacg
     1021 tcgaatgaga tctcggttgt tgagactttg gctgttgacc cgacaactgt cccggagatt
     1081 tctccgggag gtcaaaggaa gaacatcccg cttgccgcca attccattca ggtaaatgga
     1141 tgtaagaacc cgcgctgcca gaacttttgc attccaccaa gcgagtatca gcaaaaacga
     1201 ggtcgcggta atctctcaga agacggctac aaggtctcag gagccagttc gaccggcctc
     1261 atttgtcgtc attgcgataa atccagccga atcaaaagca accaagccat agccgaggaa
     1321 tacgaacggc aaagtgccta ccttcaacca caagcacgcc tttgctgccc gaatccatct
     1381 tgcgcgaacc atataccgac aaaccaggtt ggcgataccg ggttccgtcg attcggaaaa
     1441 actcattccg ggtcagcacg gtggcaatgc aagctctgca agaaaacctt ctcagttgga
     1501 agttcaacgc ggtatcacaa gaagccacat ctgaatatgc aggccttcga gcttattaca
     1561 aacaaggtct ccattagcag gatgacagaa atacttggca tcagctacac gagcgtttat
     1621 ggcaaaatcg attttctcta caaccagtgc ctaacttttt ctgccagcag agagcgactg
     1681 ctatcgtcct tgacatatga taggctttac ctctccaccg acaggcagga ttacatcgtc
     1741 aattggggcg accggaagca gcggaaaacg atccaactca cgtccatcgg cactgccgac
     1801 cgctatagcg gttacgtgtt tgggctgact ccgaatttcg atgccgccgt gaacccggag
     1861 cagcttgaac gggattgggc tgcctgtggt gacggtgcga aggcaccagc catgcgtcag
     1921 ttcgctcggc tatggacctt ggacgactac gacgcctcgg tagcgaaagc cgccaacgcc
     1981 ggggacaggc aggatatcgc cacacgggac gacctcgatg cacctgagtt gctatccgtc
     2041 ggacagcaat taccgacgtc cggtgctcag gttcacgctg aatatatcat gcatggacac
     2101 tactggttgc tccggtcctt gttcccgaag gcggagaagc tccggttctt tatcgaccga
     2161 gatgccggtc tcctgcacgc ctgcatgggt gcattcggcg accgggtgaa agctcggacg
     2221 gctgatgtcg tgatcgtaga ttacctcaag gcgatcaccg tcgatgagcg gaagaaattg
     2281 ttcacggcag cccgtatctg gttcgatggg cagaaatgtc tatttccggg gttgtctgat
     2341 ggagaggtgc gaacggcaat ttttgctggg gaaatccggg ctgccaggcg gctatatgat
     2401 aataacatat cgggattgtg gtttgaccat aggtttcccg acatggggga gccgagtaag
     2461 cggttcaaat tcgtgaccga tctcgctgac tacgatgatg agcacgtagc caatctgttg
     2521 ttgctcgcaa ccctgtggcc tatcgacacg gtgttcaatc gaatcaggag gcggatggct
     2581 gcctttgaac gtccggtggc atcggttagt cgtgctcggc gcttgtggca cctgtatgcg
     2641 ccgtatgagc cagcgatggt tgagaagttg ttggtgatct accggacttg gcacaatttt
     2701 gtttgggtca gtccgaagac ggacaggaca gctgctgagt tacttggact gaccagcgga
     2761 aaaatccgag ttcaggatat cgtctacttt taactccggt ggttcgcaca ctggtagata
     2821 tgcctgttgg ctcaccagtg gtcaccacgt cctccggagc ttcggcgagg aaaacacatg
     2881 ccctctggat agacaatgcg acggactaac taacgaaacc tcatgaggtt cagcgccgga
     2941 aactcattcg agcagcgata tcacgcgagc cgcgatggct tcgtcatctt ctatccaagt
     3001 atcaacacct ttgtgtaggg gctggccgtc ggccccgctc tccttcttaa aaagcgaacg
     3061 acctgctgag cctgagcaga caaattaatg cagtcggcca gtcaaactga gcacggttcg
     3121 cgatgggcgc cctcatttat cgtggcgacg agaagttggc atgtagcttg tctaacaagc
     3181 catcatcggg cgtcaaatat cccgggggtt ccgggggcag cgcccccggg agatgatgag
     3241 ccgcgccgaa gccgctccgt gcgccccacc cgcttcccca gaat
//
LOCUS       DASRKK010000372         6743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_223254, whole genome shotgun sequence.
ACCESSION   DASRKK010000372 DASRKK010000000
VERSION     DASRKK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223254"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFC15_08200"
     CDS             complement(<1..363)
                     /locus_tag="VFC15_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZM10177.1"
                     /translation="MKIVQFCQAGPSSVLEYIDVPIPSLNDGEVLVRAEYIGVGIPDV
                     LIRSGLYGWMPPLPAVPGTEMSGIIEKVGRGVTSLKNGQRVVVSARERKHRGGCYAEY
                     NATPAESVFVLPDDADLEQ"
     gene            929..1900
                     /locus_tag="VFC15_08205"
     CDS             929..1900
                     /locus_tag="VFC15_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZM10178.1"
                     /translation="MTLPRRRLLQLAAGAAALPAVSRLAWAQTYPSRPVRIVVGYPAG
                     IAPDITARLIAQSLSERFGQQVIVDNRPGAGSNIAADAVVRAAPDGYTLLAMTVTNTV
                     NSTLYDNLSFNLIRDIAPVAGTFRSPQVLEVNPSVPAKSLPEFIAYAKANPGKINYAS
                     DGNGSAPHVVGELFKAMAGVDLVHIPYRGSYLPDLLSGQVQAAFSSIATPIAYIRAGQ
                     LRALAVTTTTRSDALPDIPTVGEFVPGFEAYLWHGIGVPKKTPIEIIDKLNKEINAVL
                     SDPNMKARLVGLGGEPMSMAPAEFEKFIADESEKWAKVIKFAGIKPR"
     gene            2830..3594
                     /locus_tag="VFC15_08210"
     CDS             2830..3594
                     /locus_tag="VFC15_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM10179.1"
                     /translation="MQQSHSRRTSFVTGASYGVGAATALALAQEGYDVAISATRAENL
                     KATLVQLERTKSKVIPLVLDLGSQSSIERAVADVVSELGELDLLVNNAAVTLRKLAVD
                     VSWAEWNSVLATNVTGTFFLTQQVGRHLIERGQPGCIVNIASTHGIVGASQQSTYGIS
                     KAALIHMTKALAIEWGQHGIRVNAIAPGRMDTPSPARAIRTADPDYMRAMLARIPLHR
                     FATAEEVAAAVCYLASAEAASITGQTLVLDGALTVA"
     gene            4002..5216
                     /locus_tag="VFC15_08215"
     CDS             4002..5216
                     /locus_tag="VFC15_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM10180.1"
                     /translation="MFDIRLNDEQRAFQRLAREFAEKEIKPIALDLDSRPNWEDRIPW
                     DVLKKGSQLGFRTFVLQEENGGAGASDHLTACLIAEELAAADIGTAYYFMLTARRARD
                     WFELRMTQEQRDYFIPRFLEDDTYFTTVAIHEPDTDLGFDYYTETPADVAFRTRAVEQ
                     PDGSWVINGAKNFQTVGYLAKLLLVIAQTDDGPRAFLVEGDSPGLVRHPMSKIGRRVG
                     DNAEIFFDEVKVPKGRILGPPPPGRTDMGTHVTIAALTLGLGRAALEETIKYTQERVA
                     GGKPIAQHQAVGLDLADMATNLEAARRLIWTAAWAKDHPEAFEDGSAEYQPYEHMATV
                     FTGNAVQRLTEQGMALFGGMGVISGMPIEKYVRDAHVQKHISFQVPLKFRITETLTGY
                     KRKVNPLLIGGN"
     gene            complement(5548..>6743)
                     /locus_tag="VFC15_08220"
     CDS             complement(5548..>6743)
                     /locus_tag="VFC15_08220"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HZM10181.1"
                     /translation="HGGILSQFQETWAKHQVVNIQYGKGEHAKKNPNTGESVAGPVTF
                     SEEELARNRINTYHALKQHPLDDEWHRVQTTDLSKVEVPFLSCANWGGQGIHPRGNFN
                     GFIEGASKQKWLEVHGDSHWSLFSSGYGLALQKRFFDHFLKGIDNGWEKTPPVTLNIR
                     HPGEKFVLRYENEWPLARTQWTKFYLDPANKTLSRTPLAAEAKIEYEALGDGVTFWTP
                     PLEHDTEITGPMAAKLFVSSSSTDADLFLIVRVYDPQGKELTFMGSTDPNTPIANGWL
                     RVSHRALDPTKSTPYRPYHPHDKAVPLTPGEVYEADVEIVSSCIVVPKGWRLALSVRG
                     RDYEYTGELSDFAKKFHYATRGTGGMTHNDPDNRPMDVFGRKVSLHAGELHQSYLLVP
                     IIPTK"
BASE COUNT         1446 a         1951 c         1912 g         1434 t
ORIGIN      
        1 ctgttcgaga tcagcgtcgt ccggcaagac gaacacagat tccgcaggtg tcgcattgta
       61 ctcagcgtag cagccgcccc gatgcttgcg ctcgcgcgca gagacgacga cacgctggcc
      121 atttttcaat gacgttacgc cgcggccaac tttctcgatg atgccgctca tttcggtacc
      181 gggtaccgcc ggtagaggcg gcatccagcc atacagacca gagcggatga gcacgtcggg
      241 gataccaact ccgatgtatt ccgctcgcac caacacctcg ccatcgttca aggacggaat
      301 cggtacatcg atgtactcga gcacgctgga aggaccagcc tggcaaaatt gcacgatctt
      361 cattcgccgt cattcctgtt cactgcggcc agcgccactg ccgacggact cgcttgggtt
      421 cctgatctat aaggcggaac aaaaacgacc accgaggctg cctatctctt tgcacgtcac
      481 gaaccttggg ttgacgtggt cttaatcggc accggcgatc cggatcactt gcggacgaat
      541 atagcatctc ttctcaagct ggcgctcccg gacaccaatc gccagcaagt cacgacgctc
      601 ttcggccact taactggaat tgtactggat ctgcctcctc ataaagtcgc gcacaaaatt
      661 gagcctatca tatcgagaat tatatgatga gatttcgagg cgcatggtag cggcagccga
      721 ccagcgaagt gataatcaga taccaaggag gctcgactcg atttcgtcgt tggcccacag
      781 ttgttccggc tggccggaat tgaccactta ctctataagc agacattcgc tagcaccgtt
      841 tgaatgctcg gcaaccatta gcggaaatcg ggctcccttt cagagctact ggaagtggaa
      901 tagccttagt atcgtcaggg gcatcgtcat gacacttcca cgccgtcgat tactgcaact
      961 ggcagcgggc gccgccgctc tcccggccgt atcgcggctc gcgtgggcgc aaacctatcc
     1021 atcgcgaccg gtacgcatcg tcgtcggcta ccctgccggt atcgcgcccg atatcaccgc
     1081 gcgcctgatt gctcaatcgt tgtcggagcg gttcgggcag caagtcatcg ttgacaaccg
     1141 accgggggct ggcagcaata ttgctgccga cgcggtcgtt agggcggccc ccgatggcta
     1201 cacgctcctc gcgatgactg tgactaatac ggtcaactcg accctctacg acaaccttag
     1261 cttcaacctc atccgcgaca ttgcgccggt tgcaggtacc ttccggtcgc ctcaggtctt
     1321 ggaggtgaat ccgtcggttc cggcaaagtc gcttcctgaa ttcatcgcct atgccaaggc
     1381 caatcccggg aaaatcaatt atgcgtcaga cggcaacggg agcgcgcccc atgtggtcgg
     1441 cgagctattc aaggcgatgg caggcgttga cctagttcac atcccgtatc gcggcagtta
     1501 cctgcccgat ctgctcagcg gacaggtgca agccgcgttt agttcgatag ctacgccgat
     1561 cgcgtacatc agggcgggcc agctgcgcgc cctcgcggtg accactacga cgcgctcgga
     1621 cgctcttccg gacatcccca cagtgggaga atttgtgccg ggcttcgagg cgtacctatg
     1681 gcacgggatt ggtgtaccca agaaaacgcc gatagagatc atcgacaagc tcaacaaaga
     1741 gatcaacgcc gttctctctg atcccaacat gaaggcgcgg cttgtcggct taggcggtga
     1801 gccgatgtcg atggcacccg ctgaattcga aaaattcatc gccgatgaat ccgagaagtg
     1861 ggccaaagta atcaaatttg ccggtattaa gccgaggtga tcctggaaag ccgcgctcga
     1921 tatttcatag tttccgttac gcgaattcgg caagcacgta ttggtgatgc agccccgcca
     1981 agtttgtaat agcgacaatg gcatcgctaa atccgcgaaa ggaacagata acttttccaa
     2041 ttgaggagat taggagcaac aacggagaag gtgccgcggt gtgactatcg cgacatgggg
     2101 agtcttgtcg agaattgttg tgaccgtcct gcaggcggca cggatcctca tcgtcaactc
     2161 ctgatagcgg gtaatatagt ggtaggtatt tcagctcctg gctgtgacat gaaactttct
     2221 cgtcgtcgaa ttctgcagct ggcagcgggt accgtcctgc ttccgacctt atcgcgaatc
     2281 gcaagggcgt aagcttatcc aacgcggtcg gcgcgcatta tctccgtcta cccgctcggg
     2341 attgcacccg atgtaaccgc gcgcctagtt gctcaatcgt tgtcagaacg gctagcccac
     2401 taacgctcgg aacattcacc gacgccatgg ttcagcgacc gccagtgcaa gcattgatgc
     2461 aaaaggtgca ccgcgttcga attcccgatg acaaggtcta ctcaggcatc gcaggtcata
     2521 acgacatcgc cgtcacgacg ccacgcggat gcttcaagct ccgagcagag cgcgtgccag
     2581 gctccccggc gtggccgatg acggcgcggg atctgattac caaattcaga gattgcgctg
     2641 agcccgttct cggtccagca ggcgctgcgc aattacttga tcgtctgcaa cgctgccttg
     2701 agcttgcaga cataagcgaa atgactattg tgacggtccc ggctcacaat agcctcgagc
     2761 gatcgaagct cggcaacgtt cctgccggat caagtcgttg gtgaagagca gcacaactcg
     2821 gatacaccta tgcagcaaag tcactcaagg cgaacctctt tcgtcactgg agcttcctac
     2881 ggggtcggcg ctgcgacggc gctggcactt gcgcaagaag gctatgacgt tgctatttcg
     2941 gcgacgcggg ctgaaaacct aaaagcaacg ctagtccaac ttgaacggac caagtcgaaa
     3001 gtgattccct tggtgctcga cctaggctcg cagtcaagca ttgagcgtgc cgttgccgac
     3061 gtcgtgtcgg agctgggcga acttgacctt cttgtcaaca acgccgccgt cactttacgc
     3121 aagcttgcgg tggatgtgag ttgggcggag tggaactcgg tcttagccac gaatgtcacc
     3181 ggcaccttct tcctcacaca gcaggtaggc cgccatctga tcgaacgcgg ccagccggga
     3241 tgcatcgtca atatcgcttc cacccacgga atcgttggtg cgtcgcagca gtcgacttat
     3301 ggcatctcca aggccgcgct cattcacatg acgaaggcgc tggcgatcga gtggggacag
     3361 cacggtatcc gggtcaatgc catcgcgccg gggcgaatgg atacgccgtc gccggcgcgc
     3421 gccatacgga cggcagaccc ggactacatg cgtgcgatgc tcgcgcgcat tccactacat
     3481 cggtttgcaa cggccgaaga agtcgctgct gcagtatgct atctcgctag cgcagaggcc
     3541 gcttcgatca cgggtcagac actggttctc gacggcgccc tgactgtggc gtgaagatcg
     3601 acctaaacgt tgaagcggtg aagcgggtat tgcaggcaaa acagcgttag gattgcactc
     3661 ggcacaagca gccagagatg gacgtggtgt ctttgtcggg cgcgttcatg ttccgtaacc
     3721 tgtcttctgc gaatcacgcc cgcacgcgta cgattcgggc catagtggca ctctttaatc
     3781 gcagccgagg cggcttcaga gatatttgaa atattaggct gtacgcgcga gaataagctc
     3841 gattcgccga cgaagtgaca tattcgcaag ggttccgggc tcatacggta gacactcctt
     3901 ttcgcgcatc ctgaaaactc aggaccatgc ctcagcgatt ttctcggttg agaagatcgc
     3961 gtaagttagt ggtgtccttt tatcgaaata cggagttgat aatgttcgat atccggctta
     4021 acgatgagca gcgggcgttt caacgactgg cacgcgaatt cgccgagaag gagatcaagc
     4081 ccatcgcgct ggatctcgac agccggccga actgggaaga ccgtatcccg tgggacgtcc
     4141 taaaaaaggg ctcccaactt ggttttagga ccttcgtgct gcaggaggaa aatggtggcg
     4201 ctggcgcctc cgatcacctt accgcctgtc tgatcgccga agaactggcc gccgccgaca
     4261 tcggcaccgc ctattacttt atgctgacgg ccaggcgcgc gcgtgactgg tttgaactgc
     4321 gcatgacgca agagcagcgg gactatttca tcccaagatt cctcgaagac gacacctatt
     4381 tcacgacggt cgccattcac gagcctgata cggacctggg cttcgattac tacaccgaaa
     4441 cacccgcaga cgttgccttc cgcacacgcg cggtcgagca gcccgacggc tcctgggtca
     4501 tcaacggcgc taaaaacttt cagaccgttg gctatcttgc gaagctgctc ctcgtcattg
     4561 cgcagaccga cgacggcccc cgggcctttc tcgtcgaggg cgattcaccc ggcctcgtac
     4621 gccatcccat gtcaaagatc ggccggcgcg ttggtgacaa tgcggagatc ttcttcgacg
     4681 aggtgaaagt gccaaaaggc cgcatcttgg ggcctcctcc tccagggcgc accgatatgg
     4741 gcacccacgt cacaatcgcc gcgttgacgc tcgggcttgg ccgtgccgcg cttgaggaaa
     4801 caattaaata cacgcaggag cgggtcgccg gcggcaagcc gattgcccag caccaagccg
     4861 tcggcctgga tcttgccgat atggcgacga acctcgaggc ggcgcgacgc ctgatctgga
     4921 cggcagcgtg ggccaaggac caccccgaag cgtttgaaga cggcagcgct gagtaccagc
     4981 cttacgagca tatggcgacg gtgtttaccg gcaatgccgt gcagcggctg accgagcaag
     5041 gcatggcgct attcggcggc atgggcgtca tctcgggcat gccgatcgag aaatacgtgc
     5101 gcgatgcgca cgtccagaag cacatctcat tccaggttcc gttaaagttc aggatcaccg
     5161 aaacgctgac gggatataaa cgcaaggtca atcccttgct cattggcggc aactagggtc
     5221 ggttgaattt ttccggatgt gagggcgatg aaggtgagat tgatttctcg gccctatgaa
     5281 ctttttaaag ctggaatgtc gccttatggg gtaaagcaga cacgccaatt gaggcagacc
     5341 gccgcagcca ataaatttcc taactgatct acgtctatat ccggacgcgg cctaaaagac
     5401 tcttggatta ttctttgatt ttccgccgcg tctattcttc ccagcagcct tagttggcac
     5461 cctagagtga tcacaggcaa cgtcatccaa gcggacgaac gcgtattcga attttgtctc
     5521 aatacgcccg agttatctct tttgcaccta tttcgtcggg atgatcggca cgagcagata
     5581 agactggtgc aattctccgg catgaagcga cactttcctg ccgaaaacgt ccatcggtcg
     5641 gttgtcggga tcgttatggg tcatgccacc agtgccgcgg gtcgcgtagt gaaacttctt
     5701 ggcgaagtcc gacaattcgc cagtgtactc atagtccctg ccgcgcacgc taagcgccag
     5761 ccgccatccc ttaggcacca cgatgcagct cgacacgatc tcgacgtcgg cttcgtagac
     5821 ttcgccgggc gtcagcggta ccgccttgtc gtggggatga tagggcctgt acggcgtact
     5881 cttggtcgga tcaagggccc ggtgagagac cctgagccag ccgttggcga tcggcgtatt
     5941 cggatcggtg gagcccatga aggtgagctc cttgccctgc gggtcgtaca cgcggacaat
     6001 taagaacagg tcggcgtccg tggatgacga agagacgaac agtttggccg ccatcggtcc
     6061 ggtaatctcg gtatcgtgct ccagcggtgg ggtccagaag gtcacgccat caccgagcgc
     6121 ttcgtattcg atcttagcct ccgccgcgag cggcgtacgg ctcagcgtct tgttcgccgg
     6181 atcgagataa aacttggtcc attgcgtgcg cgcgagcggc cactcgttct cgtatcgcag
     6241 gacgaacttt tcccccggat ggcggatgtt gagggtcacg ggcggagtct tctcccaacc
     6301 attgtcgata cctttaagaa agtgatcgaa gaagcgtttc tgcagcgcta ggccgtagcc
     6361 ggacgagaac agcgaccagt gagagtcgcc gtggacttct agccacttct gcttcgacgc
     6421 accttcgata aagccattga agtttccgcg cgggtggatg ccctggccgc cccaattagc
     6481 gcaggacaga aacggcacct ctaccttgga caaatcggtg gtctgcacgc gatgccattc
     6541 atcgtcgagc gggtgctgct tgagggcgtg gtaggtattg atccgattgc gggcgagttc
     6601 ctcttcagag aacgtgacag gaccagctac tgactcgccc gtattggggt ttttcttggc
     6661 gtgctcgccc ttgccgtact gaatattgac cacttggtgc ttcgcccaag tttcctggaa
     6721 ctgactgaga ataccaccgt ggt
//
LOCUS       DASRKK010000373         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_28781, whole genome shotgun sequence.
ACCESSION   DASRKK010000373 DASRKK010000000
VERSION     DASRKK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28781"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2656
                     /locus_tag="VFC15_08225"
     CDS             <1..2656
                     /locus_tag="VFC15_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM10182.1"
                     /translation="GGGGMGEVYRARDTRLDRTVAIKVLPTHLSANPEAKDRFEREAR
                     AISSLNHPRVCTLYDVGHQDGIDFLVMEYLEGESLGERLKKGALSLKESLKVGIDVCE
                     ALEVAHRQGIVHRDLKPGNIMLTQSGAKLMDFGLAKPLAMATAGPGSGAPPSFTAAAV
                     TAAGPSPLSPLTMAGTIVGTVQYMSPEQIEGKEADPRSDIFAFGAVLYEMFAGKRPFE
                     GKSQISLASSILEKDPEPIGALKPSTPPAFEHTVTTCLQKDPEERFQTAHDVKLELQW
                     IANTSSSSAMAALPASATASTSRKRERLGWAIAFVIALALGGISGTFMDRSAPAAQVT
                     RTLMNPPENTTLNLAGDSAGPPVLSPDGSSVAFTATKPDGSTAIWVRPMDSLEARLVP
                     ATENASFPFWSPDSRSVGFFADGKLKTVDLNGNSAQVLADAPFGRGGAWGPGGVILFS
                     PATQAALMRVSLSGGAAPVTATTVDRDKHTSHRWPFFLPDGKHFLYLAIHHEPSKSAN
                     NALYYASLDGRENRALFTCQSNAVYAGGFLLFARGDQLMAQSFDPASGTLSGEPVSIA
                     KGVMNDVTTWHTDASAFEDGLLLVGNGGSGDWQLVWVDRNGKQVSVAADKLTNLQTAS
                     ISPQGDRIVLQLDSGQSDIWVIDVARGVRTRLTFGPVQNAFPVWSPDGKWIAYNSDRN
                     GHANIYRKLSDGSGPEELLLSDEQIVIPTHWTRDGKYLICDRGPAGGSEIWALPLEGE
                     RKPSLVVPHTPNSFATMGHVSPNGHWLTYTSSESGAPEVYVAGFRGPGKWQVSATGGV
                     QSKWSSDGKELYYMDSRFNLLSVPVKEVDGALQFGSPQQLVSNWSAPQVFYDVSPDGK
                     KILFNHVSQHVSQSVTVITNFTAGLKKE"
     gene            2721..3435
                     /locus_tag="VFC15_08230"
                     /pseudo
     CDS             2721..3435
                     /locus_tag="VFC15_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          799 a         1102 c         1132 g          755 t
ORIGIN      
        1 cggggggggc gggatgggcg aggtgtatcg agccagagac acacgcctgg accgtaccgt
       61 agccatcaag gttctgccca cccacctttc ggctaatcct gaagccaaag atcgtttcga
      121 gcgcgaagcc cgcgctatct cttctttgaa ccatccgcgt gtttgcacgc tttatgacgt
      181 gggccaccag gacggcatcg actttcttgt aatggagtat ctggagggag agtcgctggg
      241 cgagcggctg aaaaaaggtg cgctatcgct gaaggagtcg ttgaaggttg gaattgatgt
      301 ttgcgaggcg ctggaagtgg cgcatcgcca gggaatcgtt caccgcgatt tgaaacccgg
      361 caacatcatg ctgacccaga gtggcgccaa gctgatggac ttcggcctgg cgaaaccgct
      421 ggccatggcg accgccggcc caggctcagg agcgcctcca tcgttcaccg cggcggcagt
      481 gacagcggcc gggccgagcc ctttaagccc actcactatg gcgggcacca tcgtgggcac
      541 agtgcaatac atgtcgccgg agcagatcga gggcaaagag gccgatcccc ggtccgacat
      601 ttttgccttt ggcgctgtgc tgtacgaaat gttcgcggga aagcggccgt tcgagggtaa
      661 gagtcaaatc tcgctggcat cgtccattct ggagaaggac ccggagccga tcggcgcgtt
      721 gaagccttcc accccgcccg cgtttgaaca tactgtgaca acctgcttgc agaaggaccc
      781 cgaggagcgt ttccagacgg cgcacgacgt aaagttggag ttgcagtgga tcgcgaacac
      841 cagttcctcg agcgcgatgg ctgcattgcc ggcatcggca acggcatcga cgtcgcgaaa
      901 acgcgagcgg ctgggctggg cgattgcttt tgtcatcgcg ctggcgcttg ggggcatcag
      961 cgggactttc atggaccgtt ccgctccggc cgctcaggtc acgcggacgt tgatgaaccc
     1021 gccggagaac acgacactga atctggcagg cgattccgcc gggccaccgg tgctgtcgcc
     1081 cgacggctcc agtgtggcat tcactgcgac caaaccggat ggcagcactg ccatctgggt
     1141 gcgccccatg gattcgctgg aagcgcgcct cgtgcctgcc acggaaaacg caagctttcc
     1201 cttctggtct cccgatagcc gctcggtagg attttttgcc gatggcaagt tgaaaacggt
     1261 cgatctgaat ggcaactcgg cgcaggtgct tgccgatgcc ccattcggcc gtggcggcgc
     1321 ctggggaccc gggggagtga tcctgttttc ccctgccacg caagcggccc tgatgcgtgt
     1381 cagcctcagt ggcggcgcag ccccggtcac cgctaccacg gtcgatcgcg acaaacatac
     1441 ctcacatcgc tggccatttt tcctgcccga cggcaagcac tttctctacc tcgccataca
     1501 ccacgaacct tccaagtccg caaataatgc gctttattac gcctcgctgg atggacgcga
     1561 gaatcgcgcg ctgttcacct gtcaatcgaa cgcggtttat gcgggcggat ttctgctatt
     1621 tgctcgcggc gaccagttga tggcgcaatc tttcgaccca gcctcgggaa cgctcagcgg
     1681 cgagcccgta agcatcgcca aaggcgtgat gaacgacgtt accacctggc acacggacgc
     1741 ttcggccttt gaggatgggc tgctgctcgt cggcaacggc ggcagtggcg actggcagtt
     1801 ggtgtgggtg gatcgcaacg gcaagcaggt cagtgtcgct gccgacaagc tcaccaactt
     1861 gcagaccgca tccatttctc cccagggcga tcggatcgtt ctccagctcg acagcggtca
     1921 gagcgacatc tgggtgattg atgtggcgcg cggggtccgt acccggttga ccttcggtcc
     1981 cgttcaaaat gcttttccag tctggtcacc cgatgggaag tggatcgcct acaactccga
     2041 tcgcaatggt catgccaaca tttaccgcaa gctctccgat ggtagcgggc cggaggagct
     2101 gctgctcagc gatgaacaga tcgttattcc tacccactgg acgcgcgacg gcaagtacct
     2161 gatatgtgac cgtggtcctg cgggaggctc ggagatctgg gccttgccgc tggaaggaga
     2221 gcgcaaaccg tcgctggtgg taccgcacac ccccaattcg ttcgccacga tgggtcacgt
     2281 ttctcccaac ggccattggc tcacctacac gtccagtgaa tccggcgccc ccgaggtcta
     2341 tgtggcaggt ttccgcgggc caggaaaatg gcaggtttcg gccaccggcg gagtgcaatc
     2401 aaaatggagc agtgacggca aagagctcta ctacatggat tccaggttca acttgctctc
     2461 ggtgccggta aaagaggttg acggagcgct gcaattcggc tcgccgcagc agctggtcag
     2521 caactggtca gcgccgcagg tcttctacga tgtcagcccc gatggaaaga aaatcctttt
     2581 caaccacgtc tcacagcacg tcagccaatc cgtgactgtg atcacaaact ttacggcggg
     2641 gttgaagaag gagtaggcac aatacgagga tgtgttgcaa ctttcggggt tgctggctgg
     2701 tggcgtgaaa gaatcgcgaa atgggaaccc gacccgcgat cttcaaatgg cggcagaccg
     2761 aacccggcct catactctgt gcggtccggt ggtatcttcg ctattctctt tcgctgcgcg
     2821 atgttgagga gctgctggag gagcgcggtc tcgacgtcga ccacacaacg gtctggcgct
     2881 gggtgcaata ctacagcccc gagttggagc agcgactgcg aaggcatctc aagccgacaa
     2941 acaagtcttg gcgagtggac gaaacctacg ttcgcgtgaa gggtcgttgg tgctacttgt
     3001 accgggccat cgattccagg ggagccacca tcgatttcct gctgtcagcg tttcgcgacg
     3061 ccgatgctgc caagcgcctg tttcgcaagg cactcagcga tcgctcccat ccccagcctc
     3121 gagtgatcaa caccgacctg gcaccgattt acagctccgc gatcccccga cagtaagaag
     3181 gaagggacgc tgcgcaaccg ctgtcgacac cgacctgtgc agtacttgaa caacattctc
     3241 gaacaggatc atcgagccat caaacgtcga gttaacgcta agcagggctt ccgagaattt
     3301 caggcggctc ggcgaacgat tcaaggctac gaagcaatcc acatgatccg gaaggggcaa
     3361 gctcgatggg tggcaggaga tgaccttctt cgacagattc agttcatcga cagccttttc
     3421 gacttggcag cctaagaacg caccacagac gctcggcgac aaacttgtct gcatttgaaa
     3481 cttgcaacac taccaacgag cgaaacgatg ggagacaatc gccgagcatt cacctgacta
     3541 ctgtcgcagt ggtggctggc ttcaagcacg catgatgagg actgccatcg caccgggaca
     3601 gaaattgagg gctgatggcg attttgtggg agcccgaaat aatttcgaat tcggtgatcg
     3661 aggacggttt atagtctttt tgcacgctcc gggagactac gatgacatcg ccatatcacc
     3721 ggagtttcag attgacgagc accgaaccaa gcgcgtgaag tctcaatcga actggaaaga
     3781 gccgacga
//
LOCUS       DASRKK010000374        29155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_810569, whole genome shotgun sequence.
ACCESSION   DASRKK010000374 DASRKK010000000
VERSION     DASRKK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 29155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29155
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810569"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..748
                     /locus_tag="VFC15_08235"
     CDS             209..748
                     /locus_tag="VFC15_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10183.1"
                     /translation="MPSGLRRYQETRALHFLTFSCYRRQPFLASALSRELFELALELS
                     RVRYEFFVPGYVVMPEHVHLLMSEPERGTLASAVQAMKQSVSRKLIAGREHFWQARYY
                     DFNVWTAKKRIEKRKYIHRNPVVRGLVERPQDWAWSSLRHYMNGEAGTVEIESPWTAR
                     RREKMGLSPRVLKVQIKAR"
     gene            complement(986..1519)
                     /locus_tag="VFC15_08240"
     CDS             complement(986..1519)
                     /locus_tag="VFC15_08240"
                     /inference="COORDINATES: protein motif:HMM:NF025226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZM10184.1"
                     /translation="MALLVDDPDRISRKDAQDCYKGETPLHWASQHGHLNVAELLVAK
                     KAEVNAKDDKGDTPLHSAAKYDHKDVAEFLLANKADVNAQNNSGNMPLHAAAGFKDIV
                     ELLLSNKAEVSARGNGGTTPLHTAVEAVAKTWQTCFLPTTPTLMLRTTRVPPLCISQR
                     NVEITISQNCWWPTTLR"
     gene            complement(1651..1884)
                     /locus_tag="VFC15_08245"
     CDS             complement(1651..1884)
                     /locus_tag="VFC15_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10185.1"
                     /translation="MVDKLPATLPELLERNIGIATALIVMAMFLGFINTVVNAESEKA
                     RAIGANLTLISASGQGQMAVVREMIDAKADVNA"
     gene            complement(2998..3486)
                     /locus_tag="VFC15_08250"
     CDS             complement(2998..3486)
                     /locus_tag="VFC15_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZM10186.1"
                     /translation="MAKSVNKVILLGNLGKDPELKSTPGGIAIATFSLATSSSVKDKD
                     GNWQEKTEWHNVKAFQRTAEIAAQYLKKGNKVYIEGRLHSESWDDKETGQKKYKTEVI
                     VNELVLLGGPREGGEGGGGYSGSKSGGAKASGGFDQRVPEHEEAPAHSGAGEITDEDI
                     PF"
     gene            complement(3556..3990)
                     /locus_tag="VFC15_08255"
     CDS             complement(3556..3990)
                     /locus_tag="VFC15_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZM10187.1"
                     /translation="MQISPTFFPALNAFLNGTSAVLIGTGYYFIKTGRREAHKRMMIA
                     AVITSTLFLISYLYYHVVLRAGVTHFKGEGLWRPVYFTILISHTFLAVVVVPLVLITL
                     SRALKGNFARHKAIARYTFPVWMYVSVTGVVIFVMLYKIFVR"
     gene            complement(4145..4975)
                     /locus_tag="VFC15_08260"
     CDS             complement(4145..4975)
                     /locus_tag="VFC15_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10188.1"
                     /translation="MATRTTALPATLAPPANVGIVMPSVALWWREIVRFYRQRSRVVG
                     VIASPLLFWLVIGSGFGTSFRSSGAGGQNYLGYFFPGALIMIVLFTAIFTMMTVIEDR
                     KEGFLLSVLVAPVSRSVIVLGKVLGGATLATLQGLIFLVFAPALGIHFTLVSFALTLL
                     VIFLVAFSLTALGFIIAWPMDSTGGFHAVINLFLIPLWLLSGSMFPLSGASIWIRALM
                     RINPLTYGTDALRMLLYPGQLATNLPLGINLVVLAGFTLVMFSFCFFVANRRSTKPGL
                     "
     gene            complement(5096..6103)
                     /locus_tag="VFC15_08265"
     CDS             complement(5096..6103)
                     /locus_tag="VFC15_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM10189.1"
                     /translation="MNSRTAVPEVENHQATHDVAGASPVIQLQDLRHQYGDRVALAGV
                     SFDVRPAEIFGLLGPNGSGKTTTFRILSTLMIPSGGRALIMGRDVAKDPSGVRKQIGV
                     VFQEQSIDIKLSAEENLMLIGHVYGLHGTALKARVAEMLGRVGLADRAKEKAEAFSGG
                     MKRRLELAKGLLHHPSVLLLDEPTTGLDPGARRDLWQYLQILRDDENVSVLVTTHLME
                     EAERCDRLAIYAEGNVVALGTPSELKSEIGGDVILLEATEPDALAQHIEQRFGLPATV
                     LDNRVRLEIENGHRFVTDVVEAFPGEIEGVSVHKPSLEDVFIRRTGHRFWTEQEEETK
                     G"
     gene            complement(6116..7048)
                     /gene="cyoE"
                     /locus_tag="VFC15_08270"
     CDS             complement(6116..7048)
                     /gene="cyoE"
                     /locus_tag="VFC15_08270"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZM10190.1"
                     /translation="MSTATQPLAVPRFGFAALARDYSVLVKARVTTLIILTTWCGAYF
                     AAAKSGASSLSWTVFNALLGIGLVSAGTAAINEVMERDLDARMRRTAMRPLVTKSMGL
                     VHASIVAFGMTIGGIVYLWMTTNWQTALLTGLTSASYLLVYTPMKQVHPICTFLGAFP
                     GAMPPLLGWVAVRGRIDPEAVVLFAIMFFWQFPHFHSIAWLYREDYENAGVRMLPVVE
                     SDGRSTIRAILLYSVALIPVTLAPTLMGMTGWTYFAGALLLGLGLLWFSLRMATMKLT
                     PIAPASKLRARHLLQASVIYLPLLFALMMLNRTA"
     gene            complement(7045..8016)
                     /locus_tag="VFC15_08275"
     CDS             complement(7045..8016)
                     /locus_tag="VFC15_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZM10191.1"
                     /translation="MRTQNQQELVLTSRTIPYNQAYHRFAVFLASATLALIVLGALVT
                     SNDAGLSVPDWPTSFGSLYKIPPMVGGVKYEHGHRMFAEFVGILIIVMAVWTQRVEQR
                     KWMKVLGWIALAAVIGQGVLGGLTVLLFLPWAISTAHATLAQTIFCVVTAMALFTSRS
                     WLQDSEPIVEHRISPSTPALTAITAACVWVQLILGAAFRHTGIKLLPHLIGACVVSAM
                     LCWAVVRVLTRYSNLPELRKPAQLMLALLMVQLGLGFASYLARLQWMMNAPQPTTGIV
                     ISTVSHVAGGALLLATSVILAIQTRRMITVHAPESVMTTSSHKAVTA"
     gene            complement(8178..8645)
                     /locus_tag="VFC15_08280"
     CDS             complement(8178..8645)
                     /locus_tag="VFC15_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10192.1"
                     /translation="MTLPDAPIGEEGFVVTYFLTVADQARSKEFYVGILGGKVVSDAD
                     PSYIKLANSWIILNSGGGPTPDKPEVLLETPRDLNHVRSFLNLRVADIWACYAEWKAK
                     GAVFLTEPLDNLGEELRCYMRDPDGYLIEVGQYSQKSIDDFRQYLGETEVPSK"
     gene            complement(8657..8980)
                     /locus_tag="VFC15_08285"
     CDS             complement(8657..8980)
                     /locus_tag="VFC15_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10193.1"
                     /translation="MWVRRQPKSEWNQSPMPNGGQFARSLTAAEIMIPVKCNQHPWMK
                     MYLNVLGNPFSAVTGKDGSFELKGLPPGTYTIASVHEKYGEQEMKITVGPKDSKQDVT
                     FNFRP"
     gene            complement(9103..10146)
                     /locus_tag="VFC15_08290"
     CDS             complement(9103..10146)
                     /locus_tag="VFC15_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZM10194.1"
                     /translation="MAYTVTLIPGDGIGPEVVKATVRILEATGVKFEWESFAAGAEAY
                     AKYHEYIPKELTESIERTHVGLKGPVTTPVGGGFSSINVALRRQFELFANFRPIINLP
                     GVPTKYPDVDLIVVRENTEGLYSGIEHEVVPGVVESLKIITEKASTRISRFAFEYARK
                     FKRKKIHAIHKANIMKMSDGLFIRCSRNVSKEYPEIFYGEHIVDNTCMQLVMNPYQYD
                     VLLLENLYGDIISDLCAAFVGGLGLVAGANFGTTCAIFEAVHGSAPDIAGKDMANPTA
                     VVRSAVLMLHHLGEHDAAHHINAALAHVYTEKQHVTRDVGGKAGTTEFADAVIEALRT
                     VPVPTPLAREVEL"
     gene            complement(10169..10657)
                     /gene="nrdR"
                     /locus_tag="VFC15_08295"
     CDS             complement(10169..10657)
                     /gene="nrdR"
                     /locus_tag="VFC15_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZM10195.1"
                     /translation="MKCPFCGFENDKVVDSRESKEGESIRRRRECLKCEKRFTTYERI
                     DEIPYMVIKKDGRREKFERQKVLNGLLRACEKRPVSMSKLEQIVNDAEGLVVDSPERE
                     RKASEVGELIMNRLKNIDKVAYVRFASVYLDFKDVREFMEELKELLDTKENTAAKSKI
                     VN"
     gene            complement(10752..11804)
                     /locus_tag="VFC15_08300"
     CDS             complement(10752..11804)
                     /locus_tag="VFC15_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM10196.1"
                     /translation="MNKKRILGTAIVLAVLCLLIYLQVHTWKKFDWSTFWNNTSHVNW
                     AFVLLATAVTYYTYVLRATRWRIFLKPSCSTTTARLLGPQFIGFTGLALLGRPGEMIR
                     PYLIARKENQTFSSQVAVWLVERIFDMGAVALMFVVAGFVGDPLWSTLPNHNLASEVR
                     WSAGLFLCGIVVLAAVAVVLRRSGYAIAAYLEKRFAGHKHKFAHGLSSKIVSFTDGLQ
                     VISDTSSFLQLFSLSVAMWALIAVAYWLLTHAYGGELAQLGPASIMLLMVSAMFGSLI
                     QLPGVGGGSQLATIAVMNKIFGVDNEVAVSCGMLIWVATFMSVIPIGLLLARREHLSL
                     RAVAKAEEKAEAALAE"
     gene            complement(11894..12652)
                     /locus_tag="VFC15_08305"
     CDS             complement(11894..12652)
                     /locus_tag="VFC15_08305"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM10197.1"
                     /translation="MCRVFQLALLCVLTVPQLPAAEIANLRNGYSITNEHHETLGETT
                     RLYVGGDGASSYVEVATSQIESFEPAPSEPQQLSPASPTTQDLNGIISSASARSQIDA
                     DFIASVIRAESGNNPRAVSHKGAQGLMQLMPQTASNLGVKNSLDPAQNVDGGVRYLRA
                     LLLQYNGDAAKALAAYNAGPQRVRQYKGVPPYPETHAYVARVITDYNRKKLAEQKQQP
                     KSAAYKTSASRRPTGNGSKTETEVAPGGSDPGSE"
     gene            complement(12645..13505)
                     /locus_tag="VFC15_08310"
     CDS             complement(12645..13505)
                     /locus_tag="VFC15_08310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM10198.1"
                     /translation="MKRPIRLIAIDIDGTLLNSKVELSQENHDALRRAHDAGIEIMLG
                     TGRRHDFAMPIAKSLGFDLWMMSSNGAITRSTQGETFHRDLLPKETAIKLAQSMRQYR
                     NYMVLTFDRSSMGAIVCENHDQLYGVIQRWMEKNAPFIEYVSPIENALTDDPIQAMFC
                     GPVELMDRAQEDLAACGFSQEFTALRTQYDFRNLCIVDLLNAGCSKGHALERWANHRD
                     IDRSEVMAIGDNYNDVDMLTFAGHPVIMGNASDDLKQNGWTVTLHHDDHGVAAMVDHV
                     LESNSAILNV"
     gene            13659..14648
                     /locus_tag="VFC15_08315"
     CDS             13659..14648
                     /locus_tag="VFC15_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10199.1"
                     /translation="MELRKDPITRSWVITGDEPENDHGSAYLCPFCPESGQQLQVVAS
                     MPTVNGSPWSARAVVHPHPIYRVEGEPGRAGNGLYDTMQPVGAHEVIIENARHDRQLW
                     NADDREIEEYLALCAQRIHDLKKDGRFKYVSVIKNFGGHSGQEFDHPTSEVVASTFVP
                     RRVLYELRAAREHYKLKERCVFCDIFAQEQKQEKRVIEVRGDFLACCPYASRTAYETW
                     IAPRIHEAYFERLATVPGALRDLAVLLRRTLYRIRTISDGFHMVLHTSPNTLHKSETL
                     GYWKTIQDDYHWHIEILPILGPKAKSYTFKEVYSSQVSPESAAARLRERPTEP"
     gene            14689..15051
                     /locus_tag="VFC15_08320"
     CDS             14689..15051
                     /locus_tag="VFC15_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10200.1"
                     /translation="MNVRRLKTYTAQTGYVYDYYFVGKRPALPGDPLAPSVEYIFDIS
                     SDRKTTCAVSVFLSPQVVASYAAGHGRTLSEPEQYAAVKMRLLQALDEIPDMLHDGRR
                     LTLDVETLSALLESVGID"
     gene            15200..15856
                     /locus_tag="VFC15_08325"
     CDS             15200..15856
                     /locus_tag="VFC15_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZM10201.1"
                     /translation="MFAELTPTSRKRWDVSQVTSFVVHAVVLFVLLYHSRPIFVMPSD
                     VALGRPGSSGSVIYMARTGPEKVVTAPQKPNLTVRASVPRTPSIPRPLPTVHDDNKDA
                     TETSKLDETARGGSLYGSRIPGAPLTGHEIIPALPQVFPDPAVSRADLPPGIQGDVVV
                     EVTIDEQGNVIETKLIRGIGYGIEDRVLATLRQWHFRPASKDGVTIASQHIVTFHYPS
                     "
     gene            16003..16818
                     /gene="ccsA"
                     /locus_tag="VFC15_08330"
     CDS             16003..16818
                     /gene="ccsA"
                     /locus_tag="VFC15_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZM10202.1"
                     /translation="MPLIWLRVAVAFYAVGLVYSLLLLGKRGALLAKVVEPAVGFGLI
                     FHFVSLVEYTVLVGRLSWASINYSESLLAFVAMVVFFIFFVGYRSTSLGIVIFPIVFL
                     LTFASAVGQHPVTFASPALRSSWIFVHVVMIFTGYAGLFLSFGASLLYLMQERALKAK
                     SSDGLLSKLPSLQTIDDIGYNSLLFGFPFMTFGLIAGSVLAIEKFGPLFFADPKILLS
                     FIMWIVYMLLLYTRWNSGWRGRRAALLATFAFVIALGVWAANSLSRVHRFPPL"
     gene            16815..18131
                     /gene="hemA"
                     /locus_tag="VFC15_08335"
     CDS             16815..18131
                     /gene="hemA"
                     /locus_tag="VFC15_08335"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZM10203.1"
                     /translation="MNLIVIGVNHQTAPVGVREQFAISEARLPEAVKCLASQPGIEEA
                     MIVSTCNRVELVARANGSNVDLQACLQKLYGFDPQPFRKYLYEYRERDAIRHVFRVTS
                     SLDSMVVGEPQILGQVKEAYATARAVGAVNSQLDALLSRAFAVAKRVRTETAIAMSAV
                     SIASVAVDLAKKIFGNLEGKSVYLVGAGKMCELAARHLAAHGAKKIYVGNRTYERAAA
                     LAAKFNGEAIVFDQLYDTVDRADIVITSTGAPHTIFRKEHGEKFLSRRRNRPMFFIDI
                     AVPRDVDPEMNKLDGIFVYDIDDLQQVVASHMSDRRVEADRAEAIVQLEVDKFLARLQ
                     TLDVVPTIVSLQEHLETVRQAEIDRVRGRLGALTPEQEMAVEALSRGIINKIMHTPIT
                     TLKSAARESSESTTVIDLVRKLFNLRDVRKAEADEETVSEKTGAQR"
     gene            18131..19066
                     /gene="hemC"
                     /locus_tag="VFC15_08340"
     CDS             18131..19066
                     /gene="hemC"
                     /locus_tag="VFC15_08340"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZM10204.1"
                     /translation="MALLRIGSRGSQLALWQANHISVLLRARGHEVELEIIKTTGDKI
                     TDVALAQVGTKGMFTKEIEDALAEGRVDLAVHSLKDLPTDLPPGFELVAITERVNPRD
                     VLLSVHYDGLDALPKGAKVGTSSLRRQAQLKAFRSDLVVHPLRGNVDTRVRKLEEGEY
                     DAIILAAAGLTRLGKTQLVKEYLPESFMCPAAGQGALGIEIREGDVEMRKHLEFLNDA
                     DARAATICERTLLSKLGGGCQVPIGAFAAMKDGKLQLTGVVARPDGSEVLREQQTGAD
                     PVALGELVGEMLLRRGAVKILEDVYGAAVAAPQQP"
     gene            19070..19909
                     /locus_tag="VFC15_08345"
     CDS             19070..19909
                     /locus_tag="VFC15_08345"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZM10205.1"
                     /translation="MSPDKPLSGCRVLVSRARKQAGALSSTLKALGCQVIEIPFIEIR
                     KPSSFAALDTVLHNLANYDWLILTSVNGVEALFERMARFHLDNSAFSHLKIAAIGPAT
                     KSAIEKRGLTVTVTPKEYVAESVVASLQRRVKGKRVLLVRAKVARDVIPRELQKAGAI
                     VDVIEAYETVVPEASKRRLKAALNGKRKPHAITFTSSSTVKNFVALLGLRGARAALKK
                     SAPNRGVHSASIGPVTSATLREFGLPVDIEAKEFDIPGLVAAIVAAKGRIVSAKKWTD
                     LYS"
     gene            complement(19900..21873)
                     /locus_tag="VFC15_08350"
     CDS             complement(19900..21873)
                     /locus_tag="VFC15_08350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM10206.1"
                     /translation="MRTLRDASGVLFCALLLLAAVPLFSQQPITDPAKLQSKTVENMQ
                     NFSIEKLYTTRNIGGTTWSPDGKQIAFVSNISGRNNLWLVPATGGWPTQLTVSDQRQL
                     SPAWSPDGLWIAYISDHDGDEMWDVFLVSPNGGDVVNLTTSKEIAEQEPTWAPDGKSI
                     AYTAKPRTGSSSEIELMEISTRHVRHLTQNTPKDRRNSSPIFSRDGKFVVYSQANAGG
                     KDADIFLIELAGGKTTKLTPHEGEQTYFASDLSPDGKTVLITSNAHNGYDNIGLLDIA
                     TQKIEWLTSDKWEINAGSFSPDGSTLTWAANVDGNTAIYVYDLAGKHAEALPLNNGLN
                     SLGGNPTPYSRDGSKLLYYHNGPTAPNDAWTYDLKSKSTQQVTHALPGGIRSADMVEP
                     YLVHFPSRDGKWTISAFAYIPNNIPRNGKFPAIVYIHGGPTSQFVNSFNRSIQYLVNQ
                     GYLVIAPNFRGSTGYGKEFMNANYRDEGGGDLNDIVDAAEWISKSGFVDKKKLVSMGG
                     SYGGYLTMMAVTKYPDMWAAGVSIVPYVNWFTELATEDPAIREYDISIMGDPVQNKAL
                     YEDRSPINFIDRITAPLLLIAGGNDVRDPRGEAEQVAAAIKKKGGVVQLKIYENEGHQ
                     FSRVENQIDAYQRVADFLKVRVPSPGCGCSIYE"
     gene            complement(22418..24037)
                     /locus_tag="VFC15_08355"
     CDS             complement(22418..24037)
                     /locus_tag="VFC15_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10207.1"
                     /translation="MARIEKTVFISYRRTNAPWVLAISQNLTHNGYDVFFDYTGLASG
                     NFETAILDNIHARAHFLVLLTPSALERCGDPGDWLRREIEAALDSKRNIIPLMLEGFD
                     FGTPTIANQLTGKLASLRKFNAMTLSAEYFDAAMEKLRNKFLNVALETLVQPASPEAS
                     QVATDERSAVASAPAVAEEELTAQQWFERGISATDRDEAIRFYSEAIRLQPDFAGAFN
                     NRGAARHIKGDLDSALADYDEAIRLEPDLEKAFYNRGIARKANGDLDGALEDYNEAIR
                     LKPDYSDALGNRGIVSQAKEDLEGALADYDEAIRLKPGNPINFNNRGVARYAKGDSDG
                     ALADFDEGIRLQPDYAIAFNNRGEAHYNKGDLEGALADYDKAIRLQPDNGGFYFNRGI
                     ARMAKGDLDDALADYDEAFRLQPDVADAFYNRGNARDDKGDLDSALRDYNEAIRLKPD
                     FAIGFNNRAITHLRKGDLGPALHDCNEAIRLKPDFAEAFDSRGEARQANGDLEGALAD
                     FDQAIRLQPGHAAALRNRELVLKAIADRSKS"
     gene            complement(24051..24353)
                     /locus_tag="VFC15_08360"
     CDS             complement(24051..24353)
                     /locus_tag="VFC15_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10208.1"
                     /translation="MSETLIVYNKKNIFNSFLSNTRILLTDPGRKNLLFTFLSDLSPF
                     LVLCRFILRPQLERFHSCSIPVEAPGFSPVKRLLLSVLGFSPGACAFARDVRIHQL"
     gene            24369..26423
                     /locus_tag="VFC15_08365"
     CDS             24369..26423
                     /locus_tag="VFC15_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10209.1"
                     /translation="MEPKKVTVQLQLRYEWKEPNASRLRKPLEDLIAALPPANWVNAN
                     VTQAASAAGEFEQQTDFSNVPEWLDVLGEAAKSYNLSLSMGPNSLAVSIAASGLIVGI
                     RGEAERAKEIRVQVDRFAATVGLKAIQMTPPDVALRTSGFRRSGSYRLLEDVTASDWL
                     GAVELFRTWIGETQMFTGTIVMKADPGTTQELRSVEKWKEIVTARWDDLTTVGMFLFR
                     TGRAADLRVQFADRTVNLNLTAEDSQAVQEAFTGFESKLKVAPRVAIASDAEFKGERR
                     RYYTAAPITAEWVEKALMPLLARNPAKRTGFGGTFRVADQEYTVQDFNAWMKEVELRW
                     KDMQAAGCWLTTADSRQSVDVDFEREQVTVELRNRPGGSGPAVTTFVDYESELKLTPA
                     PARPYQYYRFARTYRKLNDWDSGSDQALADAIDKAVVATFGDRRYAFMNGSMTEGEGA
                     EMQTPNSSKDEFLTRLRNGVPYVNGRIYLQGPHGYDLGVQLQRNKKKVVLRSSIPDAE
                     LFKKVALPFNKIHDLEEFEAENRNAAGEEQKSPSRIGTWMPLISGVPGFVLAALALLA
                     STLPKQTSTLEIGLPRDASEISGRSCRVEWTVTRKTILHGETWETPRAEVLVTKEGDP
                     DYRDDKRDASSGVKIDFPSDGVYDVTVTAPSVPQRSLRVTAKTPAPAPSPTPTAKTSK
                     KN"
     gene            complement(26447..27001)
                     /locus_tag="VFC15_08370"
     CDS             complement(26447..27001)
                     /locus_tag="VFC15_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM10210.1"
                     /translation="MASLTELNLADTIAVLSRTPATLDALLRGLPNFWVRSNEGNDTW
                     SPFDIVGHLIAGERNDWMPRLRTILENGESRTFDPFDRFAQAKETQDKSLEQLLDEFA
                     RLRRQNLTALQSLNLQPEDLTRRGRHPSLGVVTLAELLATWAAHDLTHLHQLSRVMAY
                     QYRDAVGPWSVYLGVLQCTGHSAG"
     gene            complement(27126..27986)
                     /gene="nth"
                     /locus_tag="VFC15_08375"
     CDS             complement(27126..27986)
                     /gene="nth"
                     /locus_tag="VFC15_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZM10211.1"
                     /translation="MPRGTIPRQPSKAGRHDKAKTSRIAAKPLKITPPKNAKPQPANL
                     APPKPPAAKSAASAPKAYNPIAPSRVREILRRLDARYPDVQCALHHNSAWELLVATIL
                     SAQCTDVRVNMVTPTLFKKYPTLEHFAALQPEELEPDIRSTGFFRNKSKSVVGAARKI
                     LSDFGGEVPRTMEELLTIPGAARKTANVVLGSWFGIAAGVVVDTHVQRISRRLELTKN
                     EDPRKIEQDLMKVIPRDKWIDFSHQIIHHGRAICIARKPKCAECPLEDLCHAADKTWS
                     TVEIHKNAKL"
     gene            28323..>29155
                     /gene="hemQ"
                     /locus_tag="VFC15_08380"
     CDS             28323..>29155
                     /gene="hemQ"
                     /locus_tag="VFC15_08380"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HZM10212.1"
                     /translation="MASKADVQAVATELPAMPLTLEGYSVLHQMFRFRWIAWRKLDEA
                     RRAEIAREVAEAFAASEEAGRFAAYSMLGHKGDLMLVHFRDSFDDLNQAELLLSRTGL
                     QEFLEPTHSYLSVIELGLYDSSVKLFRQLTERGLVPLTEEWNKEIEETIERQKQAMRP
                     RLFPKIPVSRYLCFYPMDRRRGEDKNWYTLPIEERGRQMEEHGMVGRRYAGKVQQIIT
                     GSIGFDDWEWGVDLFAPDPLVFKKLIYEMRFDHVSAVYALFGQFFVGMRVPVAKLGEW
                     AG"
BASE COUNT         6046 a         8256 c         8752 g         6101 t
ORIGIN      
        1 ggatggacca acgggacatt cctggcgctg tgtcacgcgc ttccggctga ccagcagaag
       61 caggtgctgg aagggaagtg aagcgctgaa tcgaactgag cccgggttct cccaggcact
      121 gggtgccccg ttctaccgaa ggtagggcgg ggcattttac tgtacccaca tcactgacac
      181 gcttccgcac tttcggtaat gttgccacat gccttcgggt ctgaggcggt accaggaaac
      241 tcgtgcccta cacttcctga cgttcagttg ctaccgtcgc cagccgtttc tggccagcgc
      301 tctctcgcgc gagctgttcg aattggcgtt ggagctgtcg cgagtccgct acgagttctt
      361 cgtccccgga tacgtggtga tgcccgagca tgttcacctt ctgatgagtg agcccgaacg
      421 tggcacactc gccagcgctg tgcaggcgat gaagcagtcg gtgtcccgaa aactgattgc
      481 tggccgagag cacttctggc aggcccgcta ttacgacttc aatgtctgga cagcgaagaa
      541 gcgaatcgaa aaacgaaagt acatacaccg caacccagta gtgcggggac tggtcgagcg
      601 gccccaggat tgggcatgga gcagcctccg gcattatatg aatggagaag cgggcacggt
      661 ggaaatcgag tctccatgga cggcgcgccg ccgcgagaag atgggactgt cgcctcgcgt
      721 gcttaaggtg cagattaaag ctcgatgagt gaagaaaagc cccgccctac ctgcggtaga
      781 acggggcaca caagttagag tttgggttgg ccgggccacc cgccttgaca agcccttcga
      841 ccttatcgag ggccacccgc cagagagatt taaggtactc gaataaacgt gtgtccgccg
      901 tgctggcata acatcttgac cacgtcatag tgaagttgaa tcgctgcccg gtccgcgggc
      961 gtcaagccgg cggcgtcctt ggcgtctacc tcagcgttgt tggccaccag cagttctgcg
     1021 atatcgttat ctccacgttc cgctgcgaaa tgcaaagggg tggtactctt gtcgtcctta
     1081 gcattaacgt cggcgttgtt ggcaagaagc aggtctgcca cgtctttgcg accgcctcga
     1141 ccgcagtgtg caacggcgtt gtgccgccgt tgcccctagc actgacctca gccttattgg
     1201 atagcagcaa ttccactatg tctttgaagc cagccgccgc gtgcaaaggc atattgccac
     1261 tgttgttctg ggcattgacg tcagccttgt tggctagcaa aaattccgct acgtccttgt
     1321 ggtcatattt cgccgcggaa tgcagaggag tgtctccctt gtcatccttg gcattgacct
     1381 cagctttctt agccaccagc agttccgcca cgtttaggtg gccatgctgc gacgcccagt
     1441 gcaaaggtgt ctcgcccttg tagcagtcct gggcgtcttt tctagaaatc cggtcaggat
     1501 cgtcaacgag gagtgccatg gcccttcaac atcaccacga gcggccacgt catggagatt
     1561 gaactcggct ttgttggcca gcaggaattc agccacctcc aggtggccat gctgcgaggc
     1621 caacatcaga gcggtttggc catcagcatc ctaggcgttc acatcggcct ttgcgtcgat
     1681 catttctcgt accaccgcca tctggccttg ccccgatgct gagattagag ttaggttagc
     1741 gcctatagct ctcgctttct cactttcggc attgacaacc gtattaataa agcccagaaa
     1801 catggccatc acgattaggg ccgtagctat gccaatattc cgctcgagca gttccggcaa
     1861 agtagcgggg agtttgtcga ccatagaaac ctcatgattc atgaccaaag gaattacgga
     1921 tattgaaaca ccccgttgta ttaggtacag ttttagacca tactcctcgc cttcccttac
     1981 gtaatcgcgc tgcgatttag gccagacaag ataataggta taagccgaga cgccgcgttc
     2041 tcggaaaaca taccattgtt tgtcgtgtga ggttacatag tcgtaggggc tatcttcgcc
     2101 tttgaatagg aagaagagag cgaaaacact gcaggccaat atactgactg cacaccaccg
     2161 tctgattcgc caacggccac ggattttcga cggaagcacc tgaaaccctc cgggaggaaa
     2221 gcgtcgaaag gatatcagaa atcaaacggg ttcaccgtga cacgctcgcc cggcacccca
     2281 tcgggccgat cggtctgccc gcaatacagc tcttcttaat actgggtgcc ctgttctacc
     2341 gaagagcctg ccctgagcgg agtcgaaggg tagggcgggg catttttctg cacccacatc
     2401 actgacatgc ttccgcactt tcggtagtgt ttccacatgc cttcgggtct gaggcggtag
     2461 caggaaactc gtggcgtcct ttcgtctggg cgctaacctg gaattcgaaa tcgttcccca
     2521 tccgaccttg gttactttgg tttctttcat cctccgattt ctcttgcctg ctgcttccac
     2581 ggtgcttaca aacgaattgt gggctaggga taagtgtctc tacccgcacc gggcctcgac
     2641 aagtgggcac ttggctcttt gacaaatttc catttcggaa ctcgcggtac ctaaagcgcc
     2701 gcagacagcg ccggtaagct ctttgtttgt gcgacttttc ctgtaagtag ctttgcttgg
     2761 aataacttac gcacaattgt gccgtaagtc ctttgttttc aagatggctg gggggagggg
     2821 gtgccagggg gtacccctcc catatccggg cgcactagtg gtcagcatca tgaacagggt
     2881 caagtcgagg tgcgattcag gggtcgtgta gggcgaaaca cttccgacaa aatgccccgc
     2941 caagttgtca accgggcggg gcaagttcca tctgatcgct gagtgctagt cgctgtgtta
     3001 gaacggaata tcttcgtccg taatctcgcc cgcgccgctg tgggcgggag cttcttcgtg
     3061 ctcggggacg cgctggtcga agccgccgga tgcctttgcg ccgccggatt tcgagccgct
     3121 gtagccgccg ccgccttcgc cgccctcgcg cggtccgccg agcagcacca gttcattgac
     3181 gatgacttcc gtcttgtact tcttctggcc ggtttccttg tcgtcccagg attcgctgtg
     3241 cagacgaccc tcgatataca ccttattgcc tttcttcagg tactgggcgg caatctcggc
     3301 ggtgcgctgg aaggccttga cgttgtgcca ttcggtcttc tcctgccagt tgccgtcctt
     3361 gtctttcacc gacgaactgg tggccaggga aaatgtggca atcgctatgc cgccgggggt
     3421 ggatttaagt tccggatctt tgccgaggtt gccaagaagg atgactttgt taacgctctt
     3481 tgccatgaga acagctcctg tctgcggtgc ggaatgtgct gtgaactgca aaggatatca
     3541 gaaagcggcg ggcggctaac ggacgaaaat cttgtacaac attacgaaaa taaccactcc
     3601 ggtaaccgac acgtacatcc agacgggaaa cgtgtagcgc gcaatcgcct tgtggcgggc
     3661 gaaattgccc ttcagcgcgc gggaaagggt gatcagcacc aacggaacca ctactaccgc
     3721 caaaaacgtg tgtgaaatca ggatcgtgaa gtacacgggc cgccataagc cctcgccttt
     3781 gaaatgggtt acgcccgccc gcaacacaac gtggtagtac aggtacgaga tcaggaacag
     3841 cgttgaggtg atcacggccg cgatcatcat gcgcttgtgc gcttcgcggc ggccagtttt
     3901 gatgaagtaa tagccggtgc cgatcaggac ggcactcgtg ccgttgagaa aggcgttaag
     3961 cgcggggaag aatgtggggg agatttgcat aagctgcgaa gagcagatcc cccgcccgcc
     4021 gtggcgggcg agggatgaca aacataaagg attcttgcgg cacgactgaa gtcgtgccct
     4081 gatacaaagc gatccagagt ccagcctttc agcgggcttc agcccgcaag gctctatatc
     4141 gcgctcacaa gcccggcttg gtgctgcggc ggttagccac gaagaagcag aagctgaaca
     4201 ttaccagcgt gaatccagcc agcaccacga ggttgatgcc aagtggcagg ttggtggcga
     4261 gctgtccggg atagagcagc atacgcagag cgtcggtgcc gtaggtcagg ggattgatgc
     4321 gcatcagagc gcgaatccag atggacgccc ccgacaatgg gaacatcgat cccgaaagca
     4381 gccacagcgg aatcaggaac agattgatta cggcgtggaa gccgccagtc gagtccatcg
     4441 gccacgcgat aataaagccc agagcagtca gcgaaaacgc cacgagaaaa atcaccagca
     4501 gggtcagcgc aaagctaacg agggtgaagt gaatgccgag cgcgggagcg aagacgagga
     4561 aaataagtcc ttgcagtgtc gccagcgtgg ccccgccgag caccttgccc agcacgatca
     4621 cggagcggct gacgggcgcg accagcaccg acagcaggaa gccttccttg cggtcttcga
     4681 tcaccgtcat catggtgaag atggcggtga acagcacgat catgatcagc gcgccaggga
     4741 agaagtagcc gagatagttc tggcctccgg ctccgctgga gcggaaggag gtgccgaagc
     4801 ccgagccaat caccagccag aaaagcaacg gggaagcgat gacgccgacc acgcggctgc
     4861 gctggcgata gaatcggacg atctcgcgcc accacagggc gaccgacggc ataacgatgc
     4921 cgacgttcgc gggaggagcg agtgttgcgg gtaatgctgt tgttcgagta gccataagat
     4981 ttctactacc tccccacgga ggcacggagg gcacggagaa ttaggattta aaaagcaaga
     5041 ccgattgcgg agctttcaaa aactcagctt gcccgtgcct gtctggcttt gtggttcagc
     5101 ctttcgtttc ctcttcctgc tcggtccaga accgatgccc ggttcgccgg atgaacacgt
     5161 cttccaggct gggcttgtgc accgacacac cttcgatttc gccggggaac gcttccacca
     5221 cgtcagtgac aaagcgatgg ccgttttcga tttccagacg cacgcgatta tcgaggactg
     5281 tggccggcaa tccgaagcgc tgttcaatgt gttgcgccag cgcgtcaggc tcggtggcct
     5341 caagcaaaat gacgtcgcca ccgatctcgc tcttcaactc cgacggagtg cccagcgcca
     5401 ccacgttgcc ttcggcataa atcgccaagc ggtcgcagcg ttccgcctct tccatcaggt
     5461 gtgtggtgac gagcaccgag acgttttcat cgtcgcgcaa gatctgcagg tactgccaga
     5521 ggtcgcgccg tgcgcccgga tcgagtccag tggtcggctc atcgagcagc agcaccgatg
     5581 gatgatgcag cagtcctttg gcgagttcca gccggcgctt catgccgccg gaaaacgcct
     5641 cagctttttc tttggcgcga tcggcaagtc cgacgcggcc cagcatctcc gccacgcggg
     5701 ccttcagcgc ggtgccgtgc aatccataaa cgtggccaat cagcatcagg ttttcttccg
     5761 cagagagctt gatgtcgatg ctctgttcct ggaagaccac accgatctgc ttgcgaaccc
     5821 cgctggggtc tttggccaca tcgcgtccca tgatgagcgc gcggcctccc gacggaatca
     5881 tcagcgtcga gagaatgcga aaggtcgtgg tcttgccgct gccgttaggg ccgagcagtc
     5941 caaagatctc cgcaggacga acatcgaacg acacgccagc cagggcgacg cggtcgccat
     6001 attggtggcg aagatcctgc agttgaatta cgggcgaggc tcccgcgacg tcgtgtgtgg
     6061 cttgatgatt ttcgacttcc ggtactgcag ttctggaatt caagatagct ctattctatg
     6121 ccgtgcgatt gagcatcatc agcgcgaaca gcagcggcag gtagatcacc gaagcctgca
     6181 acagatgacg cgctcgcaac ttggacgcgg gtgcgatggg agtgagtttc atggtggcca
     6241 tgcgcaggct aaaccacaac agtccgaggc ccagcagcag cgcgccggca aaatacgtcc
     6301 agccggtcat gcccatcagc gtgggagcca gagtcaccgg gatcagcgcc accgagtaca
     6361 acaggatcgc gcgaatggtg gagcgaccgt cactctccac caccggcagc atacgaactc
     6421 cagcgttctc gtaatcttcg cggtacaacc atgcaatgga gtggaaatgc gggaactgcc
     6481 agaagaacat gatggcgaac agcaccacgg cttcgggatc gatgcggccg cgcactgcta
     6541 cccatccgag cagtggaggc atggctcccg gaaacgcgcc gaggaaggtg cagatcgggt
     6601 ggacctgctt catcggcgtg tacaccagca ggtagctcgc cgaggtcaat ccggtgagca
     6661 gcgcggtctg ccagttggtg gtcatccaca ggtaaacaat tccgccgatg gtcatgccga
     6721 atgccactat cgacgcgtgc accagcccca tgctcttggt caccagcgga cgcattgccg
     6781 tgcgacgcat acgcgcgtca aggtcgcgct ccatcacttc gttgatggcg gcggtgcccg
     6841 cactgaccag gccgatgccg agcagggcgt tgaataccgt ccacgaaaga gaagaggctc
     6901 ccgacttggc ggcggcgaag tacgcaccgc accacgttgt caggatgatc aaggtcgtca
     6961 cgcgcgcctt caccagtacg gagtaatcgc gggccagagc ggcgaagccg aagcgcggaa
     7021 cagccaaggg ctgggttgca gtgctcatgc ggtgacggcc ttgtgagatg aggtggtcat
     7081 gaccgattct ggcgcgtgca cagtgatcat gcgccgtgtc tgaatcgcaa ggatcacgct
     7141 ggtggccagc agtagcgcac ctcccgcgac atgcgagacc gtgctgatca cgatgcccgt
     7201 ggtcggctgc ggagcattca tcatccattg cagccgcgcc aggtacgagg cgaatccgag
     7261 accaagttgg accatcagta gcgccaacat aagttgtgca ggcttgcgca actcaggcag
     7321 gttggaataa cgcgttagca cgcggacgac cgcccagcac aacatggcag agacgacaca
     7381 tgcgccgatg agatgaggca gcagtttgat tccggtgtgc cggaatgcag cgcccaaaat
     7441 cagctgcacc cagacacagg ctgcagtgat cgcagtcaga gctggtgtgc ttggcgagat
     7501 tctatgttca acaatcggct cggaatcctg cagccagctt cgactggtga agagcgccat
     7561 cgccgtcacc acacagaaaa ttgtttgtgc cagggtagcg tgtgcggttg agatggccca
     7621 tggcaggaat aagagaactg tcaggccgcc cagaactccc tgaccgatga ccgccgccaa
     7681 ggcgatccag cccagtactt tcatccattt gcgctgctcg acgcgttgcg tccacaccgc
     7741 catcacgatg atcaggatgc cgacgaactc ggcgaacatg cggtggccgt gctcatattt
     7801 cactccgcca accataggcg gaatcttgta cagcgagccg aacgaggtcg gccagtccgg
     7861 gaccgacagc ccagcatcat tacttgtaac cagggcgccg agcacgatca gcgccagcgt
     7921 tgcactcgcg agaaataccg cgaagcggtg atatgcctgg ttgtaaggaa tcgtccgaga
     7981 agtcagaaca agctcctgtt gattctgggt tcgcaccagc taccgcccga gtgccgaacc
     8041 aaagcctatt gaatggttgc cgggggaaaa ccaagatgct cgaatcgcac tgcatccgat
     8101 actcgaatgg gcgcagtagc cgtattgtat caaacgagat acatacctct ggaaagcccc
     8161 atcgaggcag cggagtgcta tttcgatggc acttccgttt cacccaggta ctgcctgaaa
     8221 tcatcgatcg acttctggct gtactgccca acctcgatga ggtagccgtc cggatcgcgc
     8281 atgtagcacc gcagctcctc gccgagatta tcgagcggtt cggtcaggaa cacggcgccc
     8341 ttcgccttcc attcggcgta gcaagcccag atatcggcaa ctcgcaggtt cagaaaactc
     8401 ctgacgtggt tgagatcgcg gggcgtctcc agcaatactt caggcttgtc gggcgtcggc
     8461 ccgccaccgc tattgagaat gatccacgag tttgccagct tgatatagct ggggtccgcg
     8521 tcgctcacca ctttcccgcc aaggatgccg acatagaact ccttggacct cgcttggtcg
     8581 gcgaccgtca aaaaataggt gacgacgaac ccttcttcgc cgattggggc gtcgggtaga
     8641 gtcatgcgac ctcctgttac ggccggaaat taaaagttac gtcttgcttg ctgtccttgg
     8701 gcccaaccgt gattttcatc tcctgctctc cgtacttctc atgcaccgaa gcgatcgtgt
     8761 atgtcccggg aggcaggcct ttgagctcaa agctcccgtc cttgccggtc accgcagaaa
     8821 acggattgcc gagcacgttc aagtacatct tcatccatgg atgctggttg cacttgaccg
     8881 gaatcattat ttcggctgcg gttaagctgc gcgcaaactg gccgccgttg ggcatcggcg
     8941 actggttcca ttccgacttt ggttgtcgtc gaacccacaa ttttactcgg gagcgctgaa
     9001 tgggatgcac ttcgagcaag tctggtctct tctcggtcgt agccaatgca gcgactgcgt
     9061 tccatggatt ggtatgcccg atcagagctg acgcctacga ctctacagtt cgacttccct
     9121 cgccaacggc gtcggtaccg gaaccgtgcg cagcgcttca atcacggcgt cggcaaactc
     9181 ggtcgtcccc gccttgccgc cgacgtcacg cgtcacgtgc tgcttctcgg tatagacgtg
     9241 cgccagcgcg gcattgatat ggtgcgccgc atcgtgctca cccagatgat gaagcatcag
     9301 caccgccgaa cgaaccaccg ccgtcgggtt ggccatgtct ttgccggcga tgtcaggcgc
     9361 cgagccatgc acggcttcga atatggcgca cgtggtgccg aagttcgcac ccgctaccag
     9421 tccaagaccg ccgacaaacg cagcgcacaa atccgagatg atgtcgccgt acaggttctc
     9481 cagcaacagc acatcgtact ggtagggatt catcaccaac tgcatgcagg tgttgtccac
     9541 gatgtgctcg ccgtagaaga tctcgggata ttctttcgag acgttgcgcg agcagcggat
     9601 gaacagtccg tccgacatct tcatgatgtt ggctttgtgg atggcgtgga tcttcttacg
     9661 cttgaacttg cgcgcgtact caaacgcgaa tcgcgaaatg cgcgtcgaag ccttttctgt
     9721 gatgatcttc aggctctcga ccacgccggg gacgacctca tgctcgatgc cggagtacag
     9781 cccttccgta ttctcccgca ccacgatgag atcaacgtcg ggatacttgg tcggaacgcc
     9841 cggcagatta atgatcgggc ggaagttggc gaacagttcg aactgcctgc gcagggccac
     9901 gttgatgctg gaaaagccgc cacccacagg agtggtgact gggcccttca agccgacgtg
     9961 cgtgcgctca atcgactcag tcaattcctt gggaatgtat tcgtgatact tggcgtaggc
    10021 ttcggcgccc gcggcaaaac tttcccactc gaacttcacg ccggtggctt cgagaatccg
    10081 aaccgtcgct ttgacgacct cgggtccgat tccatcaccc ggaattaacg taacggtata
    10141 tgccatagac cttcgtatgc cagagaggtt agttaactat ctttgatttc gcggcagtat
    10201 tttctttagt atccagcaac tctttcaact cttccatgaa ctctcggacg tccttgaaat
    10261 ccaagtacac cgaggcgaaa cgaacatagg ccaccttgtc gatgttcttc aatcggttca
    10321 tgatcaactc gcccacttcg ctggccttgc gttctcgctc cggggagtcg accaccaggc
    10381 cttcggcatc gttgacgatc tgctccagct tgctcatcga aaccggccgc ttttcgcacg
    10441 cgcgcagcag cccgttcaag accttctggc gctcgaattt ctcccggcga ccgtctttct
    10501 tgatcaccat gtacggaatc tcgtcgatcc gctcataggt ggtgaagcgc ttctcgcact
    10561 tcaggcattc gcgtcgccgg cggatggact cgccttcctt gctctcccgc gagtcgacta
    10621 ccttgtcgtt ttcaaaaccg cagaacggac acttcatgga tgactgaaaa ggcgggctga
    10681 ttgcgcccgc atgtcgattg taacagggga aaactggcag accttgacgt gccgcgagcc
    10741 aaagcgccgg tctactccgc caatgccgct tctgcttttt cctcggcttt cgccactgcc
    10801 cgcaaggaga ggtgttcgcg tcgagccagc agcagtccaa tgggaatcac cgacatgaac
    10861 gtagcgaccc agatcaacat gccgcaactg accgcgactt cgttatcgac cccgaagatc
    10921 ttgttcatca cggcgatggt ggcgagttgc gatccgccgc ccacgcccgg aagctgaatc
    10981 aaagatccaa acatcgcaga taccatcagc agcatgatgc tggccggtcc aagctgggcg
    11041 agctcgccgc cgtaagcgtg ggtgagaagc caataagcca cggcaatcag cgcccacatc
    11101 gccaccgaca agctgaacaa ctgaaggaac gaagacgtat cgctaataac ttgtaaccca
    11161 tccgtgaacg acacgatctt ggaactgagg ccgtgggcaa atttgtgttt gtggcctgcg
    11221 aatcgctttt cgaggtacgc ggcgatcgcg taaccggaac ggcgcagcac cactgctact
    11281 gcagctaaca ccacgatgcc gcacaggaac agacccgccg accagcgtac ttcggatgcc
    11341 agattgtgat tgggcagcgt gctccacagc ggatcgccca caaatcctgc cacgacgaac
    11401 atgagcgcca ccgcccccat gtcgaaaatg cgctccacca gccacaccgc cacctgggaa
    11461 gagaacgtct ggttctcttt gcgagcgatc aggtaggggc ggatcatctc acccggccgc
    11521 cctagtagtg ccagccccgt gaatccgatg aactgcgggc cgagaaggcg tgcggtagtg
    11581 gtcgaacacg aaggtttcag gaagatgcgc cagcgcgtcg cacgcagtac gtaggtgtaa
    11641 tacgtaaccg cggtggcgag cagaacaaac gcccaattga cgtgactggt gttgttccag
    11701 aaggtcgacc agtcgaactt cttccaggta tgcacctgca ggtagatcaa caggcagagg
    11761 actgccagga cgatcgccgt tcccaggatg cgcttcttgt tcatttgtga aaatcagcag
    11821 gcacgcgata cagcgcccaa catgaatata cagagtcaaa cggcaaacaa cgcgagggat
    11881 gggtcaaacg ctgctactcg ctgcccggat cagagccgcc cggcgccact tctgtttcag
    11941 tcttggagcc gttcccggtc ggtcgacgac tggcagatgt cttatacgcc gccgacttcg
    12001 gttgctgttt ctgctcggcc agtttcttcc gattgtaatc ggtgatgacc cgcgccacgt
    12061 aagcgtgagt ctcaggatac ggtgggacgc ctttgtattg ccgcacccgc tgcggacctg
    12121 cgttgtaggc ggccagtgcc ttggcggcat ctccgttgta ttgcaggagc aacgctcgca
    12181 gatagcgaac accgccatca acgttttggg ccggatcaag gctgttcttg actcccagat
    12241 tgcttgcggt ttgcggcatg agttgcatca gaccttgcgc gcccttgtgc gagacggcgc
    12301 gagggttgtt gccgctctcc gcacgaatga cgctggcgat gaagtcggca tcgatctggc
    12361 tgcgtgcgct ggcggagctg atgatcccat ttagatcttg cgtcgtgggc gatgcgggac
    12421 tgagttgctg cggctcagat ggtgccggct cgaagctctc gatctgcgag gtggcgactt
    12481 cgacgtagct gcttgcccca tcgccgccca catagagccg cgtggtttct cccaacgtct
    12541 catgatgttc atttgtaatg ctatatccat tgcgcagatt ggcgatctcg gccgcaggta
    12601 gctgcgggac cgtcaacaca cagagcaacg cgagttggaa aactctacac attcaggatc
    12661 gccgaattcg actccagcac atggtcgacc atggccgcca caccatggtc gtcatggtgc
    12721 aacgtgaccg tccagccatt ctgcttcaga tcgtcagacg cgttacccat gatcaccgga
    12781 tgtccggcaa aggtcaacat gtcgacatca ttgtaattgt cgccaatcgc catgacctcg
    12841 ctgcgatcga tgtcccggtg attggcccag cgctccaaag cgtggccctt cgagcatccg
    12901 gcgttcagca ggtcaacgat acacaggttg cggaagtcgt actgagtacg taaagccgtg
    12961 aattcctggc tgaagccaca ggcggcgagg tcttcctgcg cgcggtccat cagttcgacg
    13021 ggtccgcaga acatggcctg gatcgggtcg tctgtcagcg cattctcgat cgggctgacg
    13081 tactcgatga agggcgcatt cttctccatc cagcgctgaa tcactccgta cagttggtca
    13141 tggttttcgc agacgattgc tcccatgctg gaacggtcaa aggtcaggac catgtagttg
    13201 cggtactgcc gcatcgattg cgccaacttg atcgctgtct ctttgggcaa caagtcgcga
    13261 tgaaacgttt ccccctgggt ggagcgggtg atcgcgccat tcgaactcat catccacagg
    13321 tcgaaaccga gcgacttggc gatcggcatc gcgaaatcat ggcgtcgccc cgttcccaac
    13381 atgatctcga tgccagcgtc gtgggcgcga cgcagagcgt catgattttc ttgcgaaagc
    13441 tcgaccttgg aattcagtag ggtgccatca atgtcaatgg cgataagccg gatggggcgc
    13501 ttcacgtctc cattctacca agcgacactg ggttgccgcc ggattgcaaa cgattcgtcg
    13561 gatggctgcg acactttaga actccgcaag aatcaaatca gtgcaaacca aaaagcgaat
    13621 tgctcaccta gcatccagca acgtaaagta actacacgat ggaactccgc aaggacccaa
    13681 tcacgcggtc gtgggtcatc accggcgacg agccggagaa cgaccacggc agcgcttatc
    13741 tctgtccctt ctgcccagaa tcgggccagc agttgcaagt cgtcgcctcc atgccaaccg
    13801 tgaatggcag tccatggtcg gcgcgcgccg tggtgcatcc ccatcccatc tatcgggttg
    13861 agggcgagcc gggtcgcgct ggcaacggcc tgtatgacac catgcagccg gttggcgcgc
    13921 acgaggtcat catcgagaat gcccgccacg accgccaact gtggaatgcc gacgatcgcg
    13981 agatcgaaga gtacctggcg ctttgcgccc aacgcatcca cgacctaaag aaggatggcc
    14041 gattcaagta cgtaagtgtc atcaagaatt tcggcggcca ctcgggacag gaattcgacc
    14101 atcccacgtc agaagttgtg gccagtactt ttgttccgcg gcgcgtgttg tatgagttgc
    14161 gtgcggcgcg cgagcattac aagctaaaag agcgctgcgt cttctgcgat atcttcgccc
    14221 aggaacagaa gcaggaaaag cgggtgatcg aggttcgagg agacttcctt gcttgctgcc
    14281 cctacgcctc gcgcactgcc tacgaaactt ggattgcgcc gcgtatccac gaagcctatt
    14341 tcgagcgtct cgccaccgtt ccgggagctt tgcgggacct ggccgttcta ctgcgtcgca
    14401 ctctgtatcg cattcgcacc atctccgacg ggttccacat ggtgttgcat acctcgccca
    14461 acacgctgca taagtccgag accctgggct attggaagac cattcaagac gactatcact
    14521 ggcacatcga aatacttccg atccttggtc ccaaggcgaa gtcgtacacc ttcaaagagg
    14581 tatattcatc gcaagtcagt ccggaatcgg cggccgcgcg gttacgcgaa cggccgactg
    14641 agccttagtt agggccataa ggagtccaac cccgctgggg aattcgcgat gaacgttcgc
    14701 cgactgaaaa cctataccgc gcagacaggt tatgtttacg actactactt tgtcgggaaa
    14761 cggccagcgc ttccaggcga tccgcttgcc ccctcggtgg agtacatatt cgacatcagc
    14821 tccgaccgca agacaacctg tgcggtgagt gtgtttctct cgccgcaagt agtggcgtcc
    14881 tacgccgccg gccacggacg aaccctcagc gagcccgaac aatacgccgc ggtcaagatg
    14941 aggcttttac aagccctcga tgaaatccca gatatgctgc acgatggccg tcgcctgaca
    15001 ctcgatgtcg aaacgctgtc agcgttgctt gaatctgttg gcatcgatta accgcgattt
    15061 cccgatggcg acgagtgtgc cgccagccct ggatgggcgc attaatatac ccgcgtagcg
    15121 accacaatgc tcacatcctg gcttacacta agtctaattt ggagattcag gcgaaaccgc
    15181 caatgggtga ggggaccggg tgttcgcaga gctgacgccg acatcacgca aacgatggga
    15241 cgtgtcccag gtcacatcgt tcgttgtgca cgccgtggtc ctgttcgtgc ttttgtacca
    15301 ctcgcgcccc atattcgtta tgccgtcaga cgtcgcgctc ggcaggcccg gatcatcggg
    15361 tagcgtcatc tacatggctc gtactggtcc agaaaaagtc gtaacagcac ctcaaaagcc
    15421 caacctcaca gtgcgcgcgt cggtgccgag aacaccgagt attcccagac ctttgccgac
    15481 ggtccacgac gataacaagg acgccactga gacctccaag ctggatgaga ctgcccgcgg
    15541 aggttcgctg tacggctcgc gcattcccgg agcgccgctc actggccacg aaatcattcc
    15601 agcgctgcca caggtatttc ccgatccggc ggtttcgcgt gccgacttgc cacccggcat
    15661 acaaggtgat gtagtcgtcg aagtcactat tgacgaacag ggaaacgtca ttgagaccaa
    15721 acttatccgc ggaatcggat acggcatcga ggaccgcgtg ctggccacct tgcgccagtg
    15781 gcacttccgt cccgcctcga aggatggcgt gaccattgca tcgcaacaca tcgtgacctt
    15841 ccactatccc agctaacgaa ggcttcattg caggattctc cggtcccttg tgacgcaaat
    15901 cacacccggg tgtgacaagc actctgggcc ctgtcgctcc cgtgtttata ctgtttatta
    15961 cacgaacgac ataggactta tcttgtcagg tctgtggggt ttatgccttt aatctggctc
    16021 cgagttgcgg tggcgttcta tgcggtcggg ctggtctatt ccttgctttt gctgggtaag
    16081 agaggcgccc tcctggccaa ggtggtggaa cccgcggtcg gtttcgggct gatctttcat
    16141 ttcgtttccc tcgtggaata tacggtcctg gtagggcggc tctcctgggc gtcaattaat
    16201 tactccgagt ccctgctcgc gtttgtcgcc atggtagtgt tcttcatttt cttcgttgga
    16261 tatcgttcga cctcccttgg catcgtcatc ttccctatcg ttttcctact gacgttcgca
    16321 tcagctgtgg gacaacaccc ggtaacattt gcgtcgccgg ccctgcgcag cagctggatt
    16381 ttcgtccacg tagtcatgat ctttaccggc tacgccgggc tcttcctgag cttcggtgcg
    16441 agcctgctgt acttgatgca ggaacgtgcg ttgaaggcca agagttccga tgggttgcta
    16501 tcgaagttgc cgtcactgca gaccatcgac gacatcggct ataactctct gctcttcggc
    16561 ttccccttca tgactttcgg actcatcgcc ggttcggtgc tggcgattga gaaattcggc
    16621 ccgctgttct tcgccgaccc caagattctg ctctcgttca tcatgtggat cgtgtacatg
    16681 ctcttgctgt acacgcgttg gaattcaggc tggcgcggac gccgcgccgc gctgctcgct
    16741 accttcgcat ttgtcattgc gctgggcgtg tgggccgcca acagccttag ccgggtgcac
    16801 aggttcccgc ccttatgaat ctgatcgtca tcggcgtgaa ccaccagacc gctcccgtgg
    16861 gggtgcgcga gcagttcgcc atttccgagg cgcggctgcc cgaagcggtg aagtgcctgg
    16921 catcgcagcc cggcatcgag gaggcgatga tcgtctccac ctgcaatcgg gttgaattag
    16981 tggctcgtgc caacggcagc aacgttgatc tgcaggcctg cttgcagaag ctctacggat
    17041 tcgatcccca gcctttccgc aagtatctct acgaatatcg cgagcgcgat gccatccgcc
    17101 acgtctttcg cgtgacttcg agtttggatt cgatggtggt tggcgagccg cagattcttg
    17161 gacaggtgaa agaagcctac gccaccgcgc gcgctgtcgg ggccgtcaac tcgcaactgg
    17221 atgcgctgtt gtcgcgtgca tttgccgtgg ccaagcgggt gcgcaccgag accgccatcg
    17281 ccatgtccgc cgtctccatc gcttcggtcg cggtggacct cgccaagaag atcttcggca
    17341 acctggaagg taagagcgtt tacctggtcg gcgcgggcaa gatgtgcgaa ctcgcagccc
    17401 ggcacttggc cgcgcacggc gcgaagaaaa tctacgttgg caaccgcacc tacgagcgag
    17461 cggcggcctt ggccgcgaag ttcaacggcg aagccatcgt cttcgatcag ctttacgaca
    17521 ccgtcgaccg cgccgacatt gtcatcactt ccacgggagc gccccacacc atcttccgca
    17581 aggagcacgg cgagaagttc ctgagccgcc gccgcaatcg cccgatgttc ttcatcgaca
    17641 ttgcggtgcc gcgtgacgtt gatccggaga tgaacaagct tgacggaatc ttcgtgtacg
    17701 acatcgacga tctgcagcaa gtcgtggcct cgcacatgtc tgaccgccgc gttgaagccg
    17761 atcgcgccga agccattgtg caactcgagg tcgataagtt cctggcgcgt ctgcagacgc
    17821 tcgacgtcgt tccgaccatc gtgtcactgc aggaacatct ggaaacggtt cggcaggcag
    17881 agattgaccg cgtgcgcgga cggctgggcg ccttgactcc cgagcaggag atggccgtcg
    17941 aagcgctctc ccgcggtatc atcaacaaaa ttatgcatac accgattacc actcttaaaa
    18001 gcgcagcgcg cgaatcttct gagtccacga cggtcatcga tctggtccgt aagctcttca
    18061 acctgcgcga cgtgcgaaaa gccgaggccg acgaagaaac ggtgagcgaa aagacgggag
    18121 cgcaacgctg atggctctcc tgagaattgg ctctcgcgga tcgcagctcg ctctctggca
    18181 agccaaccac atctcggtgc tattgcgcgc acgcgggcac gaagtggagt tggagatcat
    18241 caagaccact ggcgacaaga tcaccgacgt tgctctcgcc caggtcggca ccaagggcat
    18301 gttcaccaag gagatcgaag acgctctcgc cgaaggccgc gtcgacctcg cagtgcatag
    18361 cttgaaagac ttgccgaccg atcttcctcc gggattcgaa ctcgtcgcca tcaccgagcg
    18421 cgtcaatccg cgcgacgtgc ttttgtcggt ccattacgac ggtctcgatg ctctgcccaa
    18481 aggcgcaaag gtcgggacca gcagcttgcg gcgacaggcg cagttgaagg catttcgctc
    18541 cgatctcgtg gtccatccgc tgcggggcaa tgttgacacg cgtgtgcgta agctcgaaga
    18601 aggagagtat gacgccatca ttctggcggc tgcggggctg acacgcctgg gcaaaacgca
    18661 actggtgaaa gagtatttgc cggaatcctt tatgtgtccg gctgcgggac aaggagcgct
    18721 cggcattgag attcgcgaag gcgatgtcga gatgcgcaag cacctggagt tcctgaacga
    18781 cgccgatgcg cgcgccgcga ctatctgcga gcgcacgctg ctgagcaaac tcggcggagg
    18841 ttgccaggtt ccgataggcg cgttcgccgc aatgaaggat ggcaagttgc aactcaccgg
    18901 agtcgtcgct cgtcccgatg gatctgaggt gctgcgcgag cagcagacgg gagccgatcc
    18961 agtagcgctg ggcgaactcg ttggcgaaat gctgttgcgc cgtggagccg tcaagattct
    19021 ggaagacgtt tacggtgcag ccgtcgcagc gccgcagcaa ccttagctta tgagccccga
    19081 caaacctctc tcgggttgcc gcgtgctggt ttcgcgcgcc aggaaacagg caggcgctct
    19141 ttcctcgacg ctgaaggcgc ttggctgtca ggtcatcgag attcccttca tcgagatccg
    19201 caagccctcg tcgttcgcag cccttgatac ggtgctgcat aatctggcga attatgactg
    19261 gctgatcctc accagcgtga atggcgtgga ggcgctgttc gaacgcatgg cgagattcca
    19321 tctcgacaac tcggcattct cgcatctgaa gattgccgct atcggtcctg cgacgaagag
    19381 cgccatcgag aagcgtggct tgacggttac cgtgacgccg aaggaatacg ttgccgaatc
    19441 ggttgtcgca tcgctgcaga gacgcgtgaa agggaagcgc gtgttgttgg tgcgagcgaa
    19501 agtcgcacgc gatgttattc cgcgggaatt gcagaaagcc ggagcgattg tcgatgtgat
    19561 cgaagcttac gaaaccgtgg ttcctgaagc gtcgaaacgg cgactgaagg ctgcgctaaa
    19621 tggaaagcgc aagccgcacg cgattacttt taccagttct tcgacggtga aaaactttgt
    19681 ggcgctgctg ggactgcgcg gggcgcgagc ggcgctgaaa aagtccgcgc cgaatcgcgg
    19741 cgtgcacagc gcatcgattg ggccggtgac ttcggcaacg cttcgcgagt ttggtttgcc
    19801 cgtcgatatc gaagcaaaag aattcgacat tccggggctc gttgccgcga tcgttgcggc
    19861 gaaggggcgc atcgtgagcg ccaagaagtg gaccgacctc tactcataaa ttgagcaccc
    19921 acatcccggc gacggcactc gcaccttcag aaaatctgcg acacgctgat aggcgtcgat
    19981 ctggttttcc acccgcgaga actggtgacc ttcgttctcg taaatcttga gctgcactac
    20041 accgcctttt ttcttgattg ccgcagctac ctgttcggct tctccgcggg gatcgcgcac
    20101 gtcgttgcca ccggcaatca gcagaagcgg cgccgtgatg cggtcaatga aattgatcgg
    20161 cgagcggtct tcgtaaagcg ccttgttctg gacgggatcg cccataatcg aaatgtcgta
    20221 ctcgcgaatg gccgggtctt cggtcgccag ctcggtaaac cagttcacgt agggcacgat
    20281 cgaaacgccc gcagcccaca tgtcgggata cttggtgact gccatcatgg tcagatagcc
    20341 gccgtaactg ccgcccattg agaccagctt tttcttgtca acgaagccac tcttcgagat
    20401 ccactccgcc gcgtcaacga tgtcgttcag gtcaccacca ccctcgtcgc ggtaattcgc
    20461 gttcatgaat tccttgccgt acccggtaga tccgcgaaag ttgggtgcga taacgagata
    20521 gccctggttg accagatatt ggatcgaccg gttgaaggag ttcacgaact ggctggtggg
    20581 tccgccgtgg atgtacacga tagcggggaa ctttccgtta cgcggaatat tgtttggtat
    20641 gtaggcgaag gccgagattg tccatttgcc atcgcggctg gggaagtgga cgagataggg
    20701 ctcaaccatg tcggcgctgc ggattccgcc gggcagggcg tgcgttactt gttgcgtgga
    20761 cttgctcttg agatcgtaag tccacgcatc attgggagcg gtgggaccgt tgtggtagta
    20821 aagcagcttc gatccatcgc gtgaataagg agtgggatta ccgcccaacg aattcagacc
    20881 gttgttgagc ggcaacgctt ccgcatgttt gccggcgagg tcgtacacgt agatagcggt
    20941 gttgccgtcc acgttggcgg cccaagtcag cgtgcttccg tcgggagaaa atgatccggc
    21001 gttgatttcc cacttgtcgc tggtgagcca ctcgatcttc tgcgtcgcga tatcgagcag
    21061 tccgatgttg tcgtaaccgt tgtgcgcgtt ggaggtgatc aggacagtct tcccgtcagg
    21121 tgagagatcg ctggcgaagt aggtctgctc gccttcatgg ggcgtgagct tcgtggtctt
    21181 gccgccggcg agttcaatca gaaaaatgtc agcgtctttg ccgccggcgt tggcctgcga
    21241 gtacaccacg aacttgccgt cacgggagaa aatcggagac gagttgcggc gatctttcgg
    21301 agtgttctgg gtcaggtgac gcacgtggcg ggtggagatt tccatcaact cgatttcaga
    21361 gctggaacca gttctgggct tggcggtgta ggcgatcgac tttccatcag gggcccacgt
    21421 gggttcctgc tcggcaatct cctttgatgt agtcaggttc accacgtctc cgccgttggg
    21481 agagaccagg aacacgtccc acatctcgtc gccatcgtgg tcggagatat aggcaatcca
    21541 caatccgtca ggcgaccacg cgggcgaaag ttggcgctga tcgctgacgg tgagctgggt
    21601 gggccatccg ccggtggcgg gtacgagcca gaggttgttg cggccgctga tgttggagac
    21661 gaaggcgatc tgtttgccat ctggcgacca cgttgtgcca ccaatgttgc gcgtggtgta
    21721 gagcttctcg atcgagaagt tctgcatatt ctcgacggtc ttgctctgga gcttggcggg
    21781 atcggtgatg ggctgctggg aaaagagcgg aacggcggcg aggagcagaa gagcgcagaa
    21841 aagcacaccc gatgcgtcgc gaagtgtacg cactcccacc cttccccaag agcggggaaa
    21901 ggatggggca cccacattct tagtgaggga agagaaaaga cccacccggg caacaccggc
    21961 ccggatgggg cacccggctt ctgtagggtt gatggggtac ccccctcccc ctcggatcct
    22021 tgttttcatg gagttacgaa cagtttccgc gcaaagtatt gagcgcgtgg gtgttatatg
    22081 gaaatgattc cggaataagg acttagccga ggacatgaca gcttgaagat atgcggaagg
    22141 gcgggaggtt ggcaagagcg gccgttgcgt cggtgtctgc atgcggtctc gttggcgagg
    22201 gacacaaatc cctcgtcctc tatgcctgtg attttaggag aatagtgggg gcggcgatag
    22261 gacccaggtc acacgtcgca gtgataaggg gcggcacgga aacagcccat ggctaaagcc
    22321 cctcaatgaa gggtgcatgg tttcgccggg ctgaaagccc agcgcttcca cccaagatgg
    22381 gacattgcat ttgcaagagt cgataatagg gaccgcgtca ggattttgag cggtcggcga
    22441 tagctttaag cacaagttcg cggttgcgca gagccgcggc atggccgggc tggaggcgga
    22501 tggcctggtc gaagtctgcg agcgcgccct ccaaatcgcc attggcctgt cgcgcttccc
    22561 cccggctgtc gaaggcctcg gcaaaatcag gcttgaggcg gatggcctcg ttgcagtcgt
    22621 gcaatgccgg ccccagatcg ccctttcgca agtgcgtaat tgcgcggttg ttgaagccaa
    22681 ttgcgaagtc gggcttgagg cggatagcct cgttgtaatc ccgcagtgcg ctgtccaaat
    22741 cgcctttatc gtcgcgcgca ttcccacggt tgtagaaggc gtcggcgacg tcgggctgga
    22801 gtcggaaggc ctcatcgtaa tccgcgagtg cgtcgtccaa gtcgcctttg gccatgcgcg
    22861 cgatgccccg gttgaagtag aaaccgccgt tgtcgggctg gagtctgatg gccttatcgt
    22921 aatccgcgag tgcgccttcc aagtcgcctt tgttgtagtg cgcttcgccc cgattgttga
    22981 aggcgatggc gtagtcgggc tggaggcgga taccttcatc aaaatccgcg agcgcgccgt
    23041 ccgagtcgcc tttggcgtag cgcgcaacgc cccggttgtt gaaattgatg ggattgccgg
    23101 gcttgagtcg gatggcctca tcgtaatccg cgagcgcgcc ctctaggtcc tccttggcct
    23161 ggctcacaat accccggttg ccaagggcgt cgctgtagtc gggcttgagt cggatggcct
    23221 cgttgtagtc ttcgagcgcg ccgtccaagt cgccgttggc cttgcgcgcg atgccacggt
    23281 tgtagaaggc cttttcgagg tcaggctcga ggcggatggc ctcatcgtag tctgcgagag
    23341 cactatccag gtcccctttg atatgtcgcg cagcgcctcg gttattgaat gccccggcga
    23401 agtcgggctg aaggcggatc gcttcgctat agaaccggat tgcctcatcc cggtcggttg
    23461 cgctgatccc tcgctcgaac cattgctggg cggttagttc ttcttccgcg accgccggag
    23521 ccgaagcgac ggctgatctt tcatccgttg cgacctgaga cgcctctggc gaagcaggct
    23581 gtacaagggt ctcaagcgcc acgttcagaa acttgtttcg caatttctcc atggcagcat
    23641 cgaagtactc cgccgatagg gtcatagcgt tgaacttccg aagggaggcc agttttccgg
    23701 tgagctggtt ggcgatggtg ggcgtgccga aatcgaagcc ttccagcatc aaaggaatga
    23761 tattgcgctt gctatcgagg gcggcttcga tctctcggcg cagccagtcg cccggatcgc
    23821 cacagcgctc caacgccgac ggggtgagaa ggacaaggaa atgcgcccgg gcatggatgt
    23881 tatccaggat cgcagtctcg aagttaccgc tggcaagacc ggtgtagtcg aagaagacgt
    23941 cataaccgtt gtgggtcaag ttctgtgaaa tcgcgagcac ccaaggagcg ttggtgcggc
    24001 gatagctgat gaagaccgtc ttctcgatgc gtgccaaagc taagctgctt tcagagttga
    24061 tgtatccgaa catctctggc aaaggcgcaa gcgccagggc taaagcccag gacgctcaaa
    24121 agcaaccttt tcaccgggct aaagcccggt gcttccaccg gaatactgca actgtggaac
    24181 cgctctagct gcgggcgaag gatgaaacga cacaatacca gaaacgggct caggtcactc
    24241 aggaaagtga acagcagatt cttgcgaccc gggtcggtca gcagaatgcg agtattgctg
    24301 agaaatgaat tgaaaatgtt tttcttgtta taaacaatga gagtttcact catattccgg
    24361 ggtgtgtcat ggaaccgaag aaggtcaccg tccaattgca attgcggtat gagtggaagg
    24421 aacccaacgc ttcgcggctg cgcaagccgc tcgaagactt gattgcggcg ctgcctccag
    24481 ccaactgggt gaacgcaaac gtgacgcagg cggcgagcgc ggcaggcgaa ttcgagcagc
    24541 agacggattt cagcaacgtt ccggaatggc tcgatgtact gggtgaggcc gcgaagtcgt
    24601 acaacctttc cctgtccatg ggcccgaatt ccctggccgt gtccattgcc gcaagcggtt
    24661 tgattgtggg gattcgcggg gaagccgagc gcgccaagga gatacgggtc caggtggatc
    24721 ggttcgcggc gacggtgggg ctgaaggcaa ttcaaatgac tccgccggac gttgcgctgc
    24781 ggacatcggg tttcaggaga agcggcagct atcggctgct ggaagatgtt acggcaagtg
    24841 actggcttgg ggcggtggag ctgttccgca cgtggatagg agaaacgcag atgttcaccg
    24901 ggacgatcgt gatgaaggcc gacccgggca ccacccagga gttacgctcg gttgagaaat
    24961 ggaaggagat cgtgacggcg cgctgggacg atctgacaac ggttggaatg ttcctcttcc
    25021 ggaccggccg cgccgccgat ctgcgggtac agtttgcgga ccgaacggtg aacctgaacc
    25081 tgacggcgga agactcgcag gcggtgcaag aggcgtttac ggggtttgag agcaagttga
    25141 aggtggcgcc gcgcgtagcg attgcgtcgg atgccgagtt caaaggagag aggaggcgat
    25201 actataccgc cgcaccgatc acggcggagt gggtggaaaa agccttgatg ccgttactgg
    25261 cgcgaaatcc ggcaaaacgg acgggattcg gcgggacctt tcgggtggcg gaccaggaat
    25321 acacggtgca ggatttcaac gcgtggatga aagaggtgga gctgcggtgg aaggacatgc
    25381 aggcggccgg atgctggcta acgactgccg attccaggca atcggtggac gttgatttcg
    25441 agcgcgaaca ggtgacggtg gagcttcgca accggcccgg cggaagcgga cctgccgtga
    25501 cgacgttcgt ggactacgaa tcggagctga agctgactcc tgcgccggcg aggccctacc
    25561 agtattaccg ttttgcgcgc acctaccgga agctcaatga ctgggacagc ggcagcgacc
    25621 aggcactggc agacgcgatc gacaaagcgg tggtggcgac gtttggagat cgccggtatg
    25681 cgttcatgaa cgggtcgatg acggagggcg agggcgcaga gatgcaaacc cctaactcca
    25741 gcaaggacga atttttgaca cggctgcgca acggcgtgcc ttatgttaat ggccggatct
    25801 atctgcaagg gccgcacggg tacgacctgg gggtccagtt gcaacgcaac aagaagaagg
    25861 tggtgctgcg gagttcgatt ccggatgccg agctcttcaa gaaagtagcc ctgccgttca
    25921 acaagatcca cgatctggag gagttcgagg cggagaaccg gaacgcagcg ggcgaagagc
    25981 agaagagtcc ttcgcgcatc gggacttgga tgccactcat atctggtgtc ccaggcttcg
    26041 tgctggcggc gttggctttg ttggccagta ctctgccgaa gcaaacgagc acgctggaga
    26101 ttgggttgcc gagggacgcg tcggaaattt ccgggaggtc atgccgagtc gaatggacag
    26161 taacgcgaaa gacgatcctc catggcgaga cttgggagac gccgagggcc gaggtgctgg
    26221 tgacgaaaga aggcgacccg gattacaggg acgataagcg ggacgccagt tcgggggtga
    26281 agatcgattt ccccagcgac ggcgtgtatg acgtgacggt aaccgcgccc tcagtgccgc
    26341 agcgaagcct gcgggtgacg gcgaagactc ccgcacctgc accttcacca acgccaacgg
    26401 cgaagacgtc gaagaagaac taagggcggc agcggatctg cgttggctac ccagcgctat
    26461 gaccagtaca ctgcaggacg cccagataaa cgctccacgg accgacggcg tcgcgatact
    26521 ggtaggccat cacgcgcgag agctgatgca gatgagtgag gtcgtgcgcg gcccatgttg
    26581 ccagaagctc cgccagcgtg accactccca gcgaggggtg gcgtcctcgg cgggtcaagt
    26641 cttcgggctg taagttcagt gactgcagcg cagtcagatt ctgccttcgc aggcgggcga
    26701 actcgtccaa gagctgctcc agcgacttgt cttgcgtctc tttcgcctgc gcaaagcggt
    26761 caaagggatc aaatgtccgg gactcgccgt tctccagtat ggttcgcagc cgcggcatcc
    26821 agtcgttgcg ctctccggcg atgagatggc ccacgatgtc gaacgggctc caggtatcgt
    26881 tgccttcgtt gctgcgcacc cagaagttgg ggagtccgcg caacagcgca tcgagggttg
    26941 cgggagtgcg gctgagaacg gcgatggtgt cggccaggtt gagttccgtc aaacttgcca
    27001 tggctggtag atgcgtgcct gacgttttgg actcaagtcc caggcgtatt cagtcgcgaa
    27061 acgtcggcga tacgtttagg ggcgggccgc gtccaaatgg tttcggaaat ctgcaacggc
    27121 catactcaaa gcttggcgtt cttgtgaatc tccaccgtgc tccaggtttt gtcagcggcg
    27181 tggcacaggt cttccagcgg acactcggcg cacttgggct tgcgcgcgat gcagatggcg
    27241 cggccgtgat ggatgatctg gtgggagaag tcgatccact tgtcgcgcgg gatgaccttc
    27301 attaggtcct gctcgatctt gcgcgggtct tcattcttcg tcagctccag ccgccgcgag
    27361 atgcgctgaa cgtgggtgtc aacgacgaca cccgcggcga tcccgaacca tgatcccagc
    27421 actacgttgg ccgtcttgcg ggcggcgccc gggatggtca gtagctcttc catggtgcgg
    27481 ggaacttctc caccaaagtc gctgaggatc ttgcgggccg ctccgacgac cgacttcgat
    27541 ttgttgcgga agaagccagt ggagcgaatg tcgggctcga gttcctccgg ctgcaatgcg
    27601 gcaaaatgtt cgagcgtggg atatttcttg aagagggtgg gcgtgaccat gttcacgcgg
    27661 acgtcggtac actgggccga caggatggtc gcgaccagca actcccatgc gctgttgtga
    27721 tgcagcgcgc actgcacgtc gggatagcgg gcgtcgaggc ggcgcaggat ttcacgtacg
    27781 cgcgatgggg cgatggggtt gtaggccttg ggcgcggacg cagcggattt cgcggctggc
    27841 ggtttgggcg gagcaaggtt cgccggttgc ggcttcgcat tcttcggtgg agtgatcttc
    27901 agaggtttgg ccgcaatgcg cgaggtcttg gctttgtcgt ggcgtccggc tttcgagggt
    27961 tggcgaggaa tggtgccgcg tggcatgagg tcgtctccag gatgagtata acgggctgga
    28021 agattgagtt tacttgactc gggaaagtaa cgaccaaagg cacagtaccg caatcagtga
    28081 gcctgctact cccacgaggc gccaaaaaac taatgatctt cctcgccatc cagagtgctt
    28141 gtcggagtcg ttatgcgcct taactcgtgc agggaataga aggatgtaca gaaaaccggc
    28201 gagtgccagt ccgaccagga cgaagcctgc ggctatgtta gccatgaaga gcttgcttcc
    28261 actccgacag attacaatca agtgagcgtc gtcatcaatt ttgttcgtta tggagtttct
    28321 gaatggcttc gaaggccgat gtacaggcgg ttgcgactga actgccggca atgccgctga
    28381 cgctggaagg ctacagcgtg ttgcaccaga tgttccgctt tcgctggata gcgtggcgca
    28441 agctcgacga ggcgcgccgg gcggaaattg cgcgcgaagt ggcggaagcg tttgcggcca
    28501 gcgaagaagc cgggcggttt gccgcgtact cgatgctggg gcacaagggc gatttgatgc
    28561 tggtgcattt tcgcgattca ttcgacgacc tcaatcaggc ggagttgctg ctgagtcgca
    28621 ccgggttgca ggaatttctg gagccgacac actcgtacct gtcggtgatc gagttggggc
    28681 tgtatgactc cagcgtgaag ttgtttcggc agcttacgga gcgcgggctg gttccgctga
    28741 cggaagaatg gaacaaggaa attgaggaga ccatcgagcg acagaagcag gcgatgcggc
    28801 cgcggctgtt tccgaagatc cccgtgagcc gctacctgtg cttctatccc atggaccggc
    28861 gtcgcggcga ggacaagaac tggtacacgc tgcccatcga agagcgcgga cgccagatgg
    28921 aagaacacgg catggtggga cggcgctacg cgggcaaggt gcagcagatt attaccggct
    28981 ccatcggctt cgacgattgg gagtggggcg ttgacctgtt tgctcccgat cctctggtgt
    29041 tcaagaagtt gatttacgaa atgcgcttcg atcacgtcag cgccgtgtat gcgctctttg
    29101 gtcagttctt cgtaggcatg cgagtgccgg tggcgaagct gggagagtgg gcggg
//
LOCUS       DASRKK010000375         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_908255, whole genome shotgun sequence.
ACCESSION   DASRKK010000375 DASRKK010000000
VERSION     DASRKK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908255"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFC15_08385"
     CDS             <1..283
                     /locus_tag="VFC15_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM10213.1"
                     /translation="GIRDHPTMPRSPWQNGYVERVIGSIRRECLDHLLVRNEAHLRRI
                     LRAYASYYNDIRTHRSLAKDAPVSRPVQRTGIISSRPILGGLHHHYVRV"
     gene            357..1511
                     /locus_tag="VFC15_08390"
     CDS             357..1511
                     /locus_tag="VFC15_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10214.1"
                     /translation="MQTVADRPEAPTAIRTDLGAIFVSLELSRSTWLVTSLSPGGGEK
                     MSKHVVRGGDVAGLLERFAQLREKTRARTGKVFSFVVIQEAGLDGFWIDRVLQNEGIE
                     SHVVDPASIATSRRRRRAKTDRIDGEALVRALLAYKRGEPRVCAMVKAPTPEEEDRRR
                     LCRERKVLIAERVRHVNRVKGLLFSQGISGYEPLRRDRRERLVTLQTGDGRLLPDHLK
                     AQVCRELDRLELLLEQIKAVEAERDVLLAAQQAPAAMLLDIKGIGPEFAAILWSEGLF
                     RHFDNRRQVASYAGLAPTPWQSGSVDREQGVSKAGNPRLRTTLIQLAWLWLRHQPQSA
                     LALWFEERVRRNGNRLKKTTIVALARKLLVALWKYVTAGVVIEGAVMKVA"
BASE COUNT          439 a          605 c          667 g          443 t
ORIGIN      
        1 gggcattcgc gatcatccca ctatgcctcg atcgccctgg cagaatggat atgtcgagcg
       61 cgtgatcggc tcgatccggc gcgaatgtct ggaccacctg ctcgtacgca acgaggcgca
      121 tctgcggcgc atcctgcggg cctatgccag ctattacaat gacatcagaa cgcatcggtc
      181 attagctaaa gatgcgccag tctctcgccc ggttcaacgg accggcatca tcagttcacg
      241 cccgatcctt ggcggccttc atcaccacta cgtgcgggtt tagatcccat ggggaagtgg
      301 atagcgaccg gcactctggc agagtgaggt tgctaccacc cactctggag gcgaccatgc
      361 aaaccgtagc tgaccgaccc gaagcaccga ctgctatccg tactgatctt ggcgcgattt
      421 tcgtctcatt ggaactcagc cgatcgacat ggctggtcac gtcgctgtcg ccaggcggcg
      481 gagagaagat gtcgaagcac gtggtgcgtg gtggcgatgt cgccgggcta ttggagcgct
      541 ttgcgcaact tcgggagaaa acgcgggcga ggacggggaa ggtcttctcg ttcgtcgtca
      601 ttcaggaggc tggcctcgac ggattctgga tcgatcgcgt gctgcagaat gaagggatcg
      661 aaagccatgt cgtcgatccg gcctcgattg cgacatcgcg tcggcgtcgg cgggcgaaga
      721 ctgacaggat cgacggcgag gcgttggttc gcgcgctgct ggcgtacaag cggggcgaac
      781 cacgggtgtg cgcgatggtc aaggcaccga ctcccgagga agaggaccgc cgccgcctct
      841 gccgcgagcg caaggtacta atcgccgagc gggtccggca cgtcaatcgc gtgaaggggc
      901 tgctgttctc acaggggata tctggctacg agccgctgcg ccgcgaccgg cgggagcggc
      961 tggttacgct ccagaccggc gatggtcgtc tgctgccgga tcacctgaag gcgcaggttt
     1021 gccgtgaact cgatcggctg gaactgctcc tcgagcaaat caaggcagtg gaggccgagc
     1081 gggacgtgct gcttgccgcg caacaagcgc cggcggcaat gttactcgac atcaagggca
     1141 tcgggccaga gttcgccgcc atcctctggt cggaagggct cttccgacac ttcgacaatc
     1201 gacgccaggt cgcttcttat gcgggcctgg cgccaacacc ctggcagagc ggatcggtcg
     1261 atcgcgaaca gggcgtgtct aaagcaggca atccgcgatt gagaacaacg ctcatccagc
     1321 tcgcctggct atggctgcgc catcagccgc aatcggcgct ggccctgtgg tttgaagaac
     1381 gggtcaggcg taatggcaac cgtcttaaga agacgacaat cgtggcgctg gcgcgaaagc
     1441 tgctggtagc gctctggaaa tatgtgaccg caggcgtcgt catcgaggga gccgtgatga
     1501 aagtcgcctg atgtgaacgc taaccaaatt ttctaatctt ccaggaccgt tagtcctggc
     1561 ggatccaggt ggacgaaccg ccgaaaacca tggcctgaaa agccgcagtt tagaatggtc
     1621 tcgtccttct gagccccgcc cgccgcaagc gggatattgg tgcagtcgct ttgcgcggcg
     1681 accggatgtg aggttgatct ggcgaggaca accagatcac ggaatcgggc ttggaccttg
     1741 gataccgaaa taggcaccgg agtcttaact catgcactaa tgattttttg cctcttgact
     1801 cggaaatccc catgtgaggt tttcggtaca cacagccgcc attgaccctg taggcatgat
     1861 tgagactggc tgagttgatt tcagcccgtg ttgctgctgt tggcgtctgc gccattgcct
     1921 gccggcgact aaggcggctg cgatttctcc aactacgaag aaatttggta ttcattgtat
     1981 tgctccaaca aatgcctgat catgaatctg aagagccgtg caagcgcagg aatggcagcg
     2041 atactggcag gcctcgttcg ccgctgattc actacactct cgatggttcc aacgccgcag
     2101 atatatcttc gggtggataa ttcgttatct gattcaaagt gactcgttgc gatg
//
LOCUS       DASRKK010000376         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_713246, whole genome shotgun sequence.
ACCESSION   DASRKK010000376 DASRKK010000000
VERSION     DASRKK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713246"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /gene="lptC"
                     /locus_tag="VFC15_08395"
     CDS             complement(<1..1308)
                     /gene="lptC"
                     /locus_tag="VFC15_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZM10215.1"
                     /translation="MFQSIAQVRKWLVVSVVVLVATVAVSYWIARSRVGPTLHNVPAQ
                     LGIDIQQTSEGFSLSKSEGGRTLYTIRASKAVQFKAGGHADLRNVHIVVYGREHDRYD
                     QIYGNQFTYDPQTGDINAVGEVHIDLQGNAEGPQKPDQAPPEELKNPIHLLTHSLTFN
                     QKTGVAHTDEVVEFRTSQANGSAKGAYYDSKSNELLLKSDVHIVTTSARPAIITGTSG
                     TIQKTPRQATLGNARIEQPDRTLTADKGTMLFEADNTVQHVLAEGNVHIQSRGPSIVD
                     ITGPRGDVNMGPNNSVAQTILSGGAKFESHGASVAHGSADRFVLDFDDQNQPLRLHMV
                     KDARMMQDPKPGKSGSSGQPGGQPMEIAADQLDFLLENGNQLKTGDTVGKATITIFPA
                     PPSSRKQKSASTGDKETGGNSTTVATAGKFHATFDSNNRMQALH"
     gene            complement(1654..2181)
                     /locus_tag="VFC15_08400"
     CDS             complement(1654..2181)
                     /locus_tag="VFC15_08400"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZM10216.1"
                     /translation="MSSQPTKPLAPSAPTDLALPEYAGFWLRAWAGAIDVALEASGAL
                     LLTFAIDLGLRRFGRLLGISLFVSKVATGMAFILILAVGSWLYCAFTESSSWRATVGK
                     RILGLQVVTAEGDRIGFGQATVRHFMKFLSLFFLTIGFMMAGWTKRRQALHDIPSDCL
                     VIRVPPEKNFSLLRG"
     gene            2547..>2804
                     /locus_tag="VFC15_08405"
     CDS             2547..>2804
                     /locus_tag="VFC15_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10217.1"
                     /translation="MIRKVSALLCLVVLASMAALAGDIPFSGSGSSGTVNPGQPFSYN
                     FDGALFEPDWGIPGVNNGLAVWSGPTIDQFTITFDLAAGIAI"
BASE COUNT          571 a          782 c          845 g          606 t
ORIGIN      
        1 gtgcagcgct tgcatgcgat tgttgctatc gaaagtagcg tgaaacttgc cagcagtagc
       61 gaccgtggtg gagttccctc cagtttcctt atcgccagtg ctggcgcttt tctgctttcg
      121 tgagctcggt ggcgcaggga agatggtgat ggtggctttg ccgacggtgt cgccagtctt
      181 gagctggttt ccattctcca gcaggaaatc gagctggtcg gcagcaattt ccatgggctg
      241 gccaccgggc tgcccgctgc taccggactt acccggcttg gggtcctgca tcatgcgcgc
      301 gtccttcacc atgtgaagcc gcaacggctg gttctgatcg tcgaaatcaa gaacaaacct
      361 gtccgccgaa ccgtgcgcaa cgctcgcgcc gtgcgactcg aatttagcgc cgcccgagag
      421 gatagtctgc gcgacagaat tgttcggtcc catgtttaca tccccgcgag ggcccgtaat
      481 gtcgacgatg gatggcccgc ggctctggat gtgaacattg ccttccgcca gcacgtgctg
      541 caccgtgttg tccgcttcga acagcatggt gcctttgtcg gcggtcagtg tccggtccgg
      601 ctgttcgatg cgcgcgttcc caagagtggc ctgcctgggt gtcttctgaa tcgtgccact
      661 ggtgccggtg atgatcgctg gacgcgcgct ggtggtgacg atgtgaacat cggacttcaa
      721 gagaagttca ttgctcttgg aatcgtagta cgcgcctttg gcagatccat tggcttggga
      781 cgtgcgaaac tccacaactt cgtcggtgtg cgcgaccccg gtcttttggt tgaaggtgag
      841 ggagtgggtc aataggtgaa tcggattctt cagttcctcg ggcggggcct ggtcaggctt
      901 ctgcggaccc tcggcgtttc cctggaggtc gatatgcacc tcacccacgg cgttgatgtc
      961 gcccgtctgc ggatcgtagg tgaactggtt gccataaatc tggtcataac ggtcgtgctc
     1021 gcgcccgtac acgacaatat gaacattccg cagatcggcg tggccgccgg ccttaaactg
     1081 caccgccttg gaagctcgaa tggtgtagag agtgcggcca ccttccgact tggaaaggga
     1141 gaacccctcg ctggtctgct gaatgtcgat gcccaattgt gcgggcacat tgtgcaacgt
     1201 gggaccaacg cgggagcggg cgatccagta cgacacagcg accgtcgcca ccagcaccac
     1261 cacactcacg accagccact tgcgtacctg agctatggat tggaacatct atgatcgata
     1321 ttacgctagc gggcgcggtc gacgaaaacg cggaaccgga gggcgaaaga ggctgctgaa
     1381 gcaagcgacg ttcgggtagt gcggaccttc aggtccgcgt ccacgccttt atttttgcca
     1441 tcccgagcgg gcttcacagg ctgcggaaag agtcggtttt cagccggtgt tttgaagggg
     1501 cgcaccttta ggtgcgcccc taaacttttg cttttgtcac cacgagcggg cgaaccgggg
     1561 tccccaacga gcgccgtttt tgcgcttgtt ggggtaaggt ccagcccgcg agggatttgc
     1621 ttttccggct ttctcagcag cctcgaaccc gcctcagccg cgcagcaggg agaaattctt
     1681 ttcgggcggt acccggatga ccagacagtc ggaaggtatg tcgtgcagcg cctggcggcg
     1741 cttggtccag cccgccatca tgaaccctat cgtcaagaag aacagggaga gaaatttcat
     1801 gaagtggcga accgtagctt gtccgaaccc gattcggtcg ccctcagcgg tgacgacctg
     1861 cagacccaaa atgcgcttac cgacggtggc gcgccacgat gaactttcgg tgaacgcaca
     1921 atacaaccac gaaccgacgg ccaggatcag gatgaaagcc atccccgtgg cgaccttgga
     1981 cacgaagagg gaaattccca gcaggcggcc aaagcggcgc agcccaagat cgatggcgaa
     2041 tgtaagcagt agcgctccac tggcctccaa ggctacgtcg atcgcacccg cccaggcacg
     2101 aagccagaat ccggcgtact ccggaagagc caagtcggtt ggcgctgaag gtgctagcgg
     2161 cttggtaggt tgagaagaca ctataccctt ggagttggac tccgaaagca agtctccgca
     2221 gaagaggcag aaccgtccgt ccggcaatcg cgcattgccg cagttggaac aaacctggtc
     2281 cagcactatc tccattcgga tgggaatact acgctggact tgttgtccct gtaaagagtg
     2341 ggcctagttc ttcggaggga ttggtgctgc gcgaaacccg gctgtgggca atttattgca
     2401 tatgatgtgc aaagttttgc actcatgccg tgcccccagg cctcaccacg cataaatttg
     2461 aaggacttat acagcaaaac agtggtaatc tacttgcaat agtgtaactg ggacacaagt
     2521 tcccacagtc tgagaggaga aagctcgtga tacgcaaagt ttctgcgttg ctgtgcttgg
     2581 ttgtgctagc ctccatggca gccttggccg gcgacattcc gtttagcgga tcgggatcca
     2641 gcggaacggt aaatccgggg caacctttca gctacaactt cgatggtgcg ctattcgaac
     2701 cggactgggg aattccgggt gtgaacaacg gtctcgcggt ttggagtggg ccgaccatcg
     2761 atcaattcac gatcacgttc gacctggccg caggaattgc catc
//
LOCUS       DASRKK010000377         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1397481, whole genome shotgun sequence.
ACCESSION   DASRKK010000377 DASRKK010000000
VERSION     DASRKK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397481"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFC15_08410"
     CDS             <1..444
                     /locus_tag="VFC15_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Involved in disulfide oxidoreductase activity and
                     electron transport; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HZM10218.1"
                     /translation="PAFTHISYDDFRIIRTNLIEKGSATTDGRLVPYTVFIDPQLGRI
                     GVTENEARKQKRNIRVAKLPMTSVARALEVDETRGFMKAIVDGETNQILGAAVLGIEG
                     GEVMSALEIAMMGKLPYTALRDGVFAHPTLAESLNNLFMAMDSGK"
     gene            818..1315
                     /locus_tag="VFC15_08415"
     CDS             818..1315
                     /locus_tag="VFC15_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZM10219.1"
                     /translation="MLQKRIDDQPTISREIVREAEAIFHGHLKRVGLKQTGQRGVILR
                     TFLDTRDHLSTDELYRLVKKHDERIGFTTVYRTLKLLAECGLASEIAFNDGVTRYEQQ
                     YNRRNHHHMVCTECGSSVEFFSRDIERIEQEVGRKNKYLPTRHTFQIYGVCERCQKKQ
                     KRSLA"
     gene            1527..2162
                     /locus_tag="VFC15_08420"
     CDS             1527..2162
                     /locus_tag="VFC15_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM10220.1"
                     /translation="MTNPKQHPTLIRGTSLRGPGFALALICVLTMVAMPRAQAQTFTV
                     LHPFTGASDGAIPYGGLTLDQAGNIYGTASAGGFTGNNCTAAGCGTVFKLTHRGSGWT
                     FAPLYIFQGDTDGVAPYAGVTIGPNGTLYGTTLLGGYGTGTLFNLSPPAHATGNVLGG
                     WTETVLYLFDNGASYPVYGKVIFDSPGTSMAQPTREVWNAPMAATVGRCSS"
     gene            2240..2788
                     /locus_tag="VFC15_08425"
     CDS             2240..2788
                     /locus_tag="VFC15_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM10221.1"
                     /translation="MVMDGSRNLYGTTSAASVTPVAYELIPSASGWTEIVLHTFGYFD
                     DPRGGVIFDGTGGLFGTTVNGAVYQLSPSGGQWIYNLLYNFSGPSGPWSDVVRDASGN
                     LYGTTCADGRFSHGSVFKLTPSQGGWTETDLYDFTGGSDGSCPVGAVAINAAGDVYGT
                     TAAGGSGCSGNGGCGVVWQITP"
     gene            2941..>3327
                     /locus_tag="VFC15_08430"
     CDS             2941..>3327
                     /locus_tag="VFC15_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10222.1"
                     /translation="MFEDSLVESSGTLSKRNPWTAALSFAMQGVLAGVLVLLPIIYTE
                     ALPGRQLTSILTEPSPPPGPAPVSTQARGVQPHPMPEDKVLRVPNGIPPRVAILHDQL
                     ASADAAGAPDLDGIPGGTGTVPNSVIS"
BASE COUNT          707 a          949 c          988 g          683 t
ORIGIN      
        1 ccggcgttta cgcatatctc gtacgacgac tttcgcatca tccgcacgaa cctcatcgag
       61 aagggaagcg ccaccaccga cgggcggctc gtgccttaca ccgtgtttat cgatccgcaa
      121 ctcggccgca ttggcgtgac cgaaaatgaa gctcgcaagc agaagcggaa cattcgtgtg
      181 gccaagctac cgatgacctc cgtcgcacgc gcccttgagg tcgacgagac ccgcggtttc
      241 atgaaggcca tcgtggatgg cgaaacgaac cagatcctgg gcgcggcggt gctgggcatc
      301 gagggtggcg aggttatgtc tgcgctcgag attgcaatga tgggaaagct gccctacacc
      361 gccttgcgcg atggcgtctt tgcccaccct actctggcgg aatcgctgaa caacctgttc
      421 atggcaatgg atagcggcaa gtagaaggcg gtaatgtgaa tcggccaggc tacggccgcc
      481 cgctgccttg aaagggcacg gcttcagccg tgccgttatg agggcgtgga attgggcttc
      541 agcccctgag ggaagtcgta cttcgcctca accccgaagt ccctcagtag ctaaagccaa
      601 tgacaacata ttacggcacg gctgaagtcg tgccccttca aagcctgaga tcctcatgaa
      661 taaatccggc cagagcgccg cactactcgg actgccggat acactattct gaatcggtct
      721 aagcttgtcc ctcgccccgc atttacagtg atatgctgat aacaaatcgt ggtactcctg
      781 caatggatag gccccgggac ggggagtagc aaatcccatg ctccaaaaac gaattgacga
      841 tcagccaacg atctcgcgcg agatagtgcg cgaggctgag gcgattttcc atgggcatct
      901 caagcgcgtg ggcctcaaac agacggggca gcggggtgtc atcctgcgca cctttctcga
      961 cacacgtgac catctctcca ccgacgaact gtaccggctg gtcaagaaac acgacgagcg
     1021 catcggattt accactgtct atcggacctt gaagctgctg gccgagtgcg ggctagccag
     1081 cgagattgct ttcaatgacg gcgtgacccg ttatgaacag cagtacaatc gccgcaacca
     1141 ccatcacatg gtctgcaccg aatgcggcag ctcggtggag ttcttttcgc gcgacatcga
     1201 acgcattgag caggaggttg gccgcaagaa caagtacctg ccgacgcgcc acactttcca
     1261 gatttacggc gtatgcgagc gctgtcagaa gaagcagaag cgcagcttgg cttgaggtca
     1321 caaatggggc atggaagagg gtcttttgtg catggagcgc cgaccgcgca catgcgaccc
     1381 tttgcaggcc caagcgtcga ttgcttgtga taaggcctga ttgcaacagt aatccccttt
     1441 tgctggaacc ccgccaagag tcgggtgtaa agtgcgaacg tcgacaacgg caaccatcga
     1501 cggtcgcata agtgaaggag accatcatga cgaacccgaa acaacatccc acattgattc
     1561 gcggcacaag cctgcgggga ccaggcttcg ccctggcact catttgtgta ctcacaatgg
     1621 ttgcaatgcc acgggcacag gcgcagacgt tcactgtgct tcaccccttt accggcgcaa
     1681 gtgatggcgc gatcccctat ggcggtctga ctctagatca ggcgggaaat atctatggga
     1741 cggcctcggc gggaggcttt acaggaaaca attgcacggc cgcgggatgt ggcactgtct
     1801 tcaagctgac gcaccgaggc tccgggtgga cctttgcccc cttgtacatc ttccaggggg
     1861 acaccgatgg cgtggctccc tatgccggag tcaccatcgg tccgaacggc acgctttacg
     1921 ggacaactct acttgggggc tatggaacgg gaacactttt caatctgagc ccgccagcac
     1981 acgccactgg gaatgtgctt ggggggtgga cggagacggt gctctacctc ttcgacaatg
     2041 gtgcatccta tcccgtttac ggaaaagtga tttttgactc gccgggaacg tctatggcac
     2101 aacctacgag ggaggtctgg aatgcaccga tggcggcaac tgtgggacgg tgttcaagtt
     2161 gaccccgtcg tccggaggct ggacgaaaac cgggtacgct tttcccggag gtagcagcgg
     2221 aggaaacccg ttgagcggcg tggtcatgga tggctcccgc aatctttacg gcaccaccag
     2281 cgccgctagc gttacccctg tggcgtacga attgattcca tcggcgtcag gctggactga
     2341 aatcgtattg cacacctttg gctacttcga cgacccccga ggcggcgtca tttttgatgg
     2401 gacgggtggt ctcttcggca ctacggttaa cggcgctgtc tatcagctct cgccatcggg
     2461 cgggcaatgg atctacaacc tgctttacaa cttctccggc cctagtgggc cttggagcga
     2521 tgtggtgcgc gatgccagcg gcaatctcta tggtacgacc tgcgccgacg gcagattttc
     2581 gcatggctct gtatttaagc tgacgccttc ccaaggcggc tggacggaga cagatctgta
     2641 cgattttacc ggtggaagtg acggatcctg tcctgtcggt gcggtggcca tcaatgcagc
     2701 cggggatgtc tacggcacaa ccgcggccgg tggaagtggc tgctcgggca acggaggctg
     2761 cggcgtcgtc tggcagatca cgccctaact gaaagcctcg gcacgctgcg tttgttgtaa
     2821 catccccata tcgcaactaa tccatcagtg cgctctaaat ctaccgcagc gcagtcgtga
     2881 ccaatcgcgg gagctaattt cgagttgccg gtgtctgtag tgaatacaag gagcgccggt
     2941 atgttcgaag acagtctggt tgagtcgagc ggaactttga gcaaacgaaa tccatggaca
     3001 gccgcgttgt cgttcgccat gcagggagtg ctggctggtg tgttggtgct cttacctatc
     3061 atctatacgg aagcgttgcc cgggcgtcag ctgacgagca ttttgacgga accttctcca
     3121 cccccagggc cggcgccggt ttccacccag gcgcgcggtg ttcagccgca ccccatgccc
     3181 gaggataaag tgttgcgggt tccgaacggg ataccgcccc gggttgcgat tttgcacgat
     3241 cagcttgcga gtgcagacgc agccggagcg cccgatttgg acgggattcc tggcggaact
     3301 gggactgtgc cgaacagcgt catcagc
//
LOCUS       DASRKK010000378         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_908594, whole genome shotgun sequence.
ACCESSION   DASRKK010000378 DASRKK010000000
VERSION     DASRKK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908594"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFC15_08435"
     CDS             complement(<1..195)
                     /locus_tag="VFC15_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10223.1"
                     /translation="MKKPFQLTSINSLRMTVLVLSGVAFLAVGFPAIAGQASTTTATP
                     SHSNVKKQASATPVKLSAIFV"
     gene            complement(250..597)
                     /locus_tag="VFC15_08440"
     CDS             complement(250..597)
                     /locus_tag="VFC15_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10224.1"
                     /translation="MENFSSKPYAGKAPLSESAQRLGSDIKSEAGGLADRAGEAAKTF
                     GDHASEAFDDLKSAGQGVVDDYATKVARGKRQIREAAGRISEYSDQNTALVVGGALLV
                     GVLLGHILTRKGE"
     gene            747..1262
                     /locus_tag="VFC15_08445"
     CDS             747..1262
                     /locus_tag="VFC15_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10225.1"
                     /translation="MKDRMDGDTKKVERAAGANPDPITGEPGSHPVGTGVGSAGGAAA
                     GVAVGAVVGGPVGALVGGVAGAVIGGGAGHAAGEAANPTVEKQYWENVYKTRPYYKSG
                     RTFDYYEPAYRYGWENATKPEFATKSFDETESVLHQRWTAQPGSATRPSWVDSKEPAR
                     DAWNRTRGRKA"
     gene            complement(1604..>2030)
                     /locus_tag="VFC15_08450"
     CDS             complement(1604..>2030)
                     /locus_tag="VFC15_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10226.1"
                     /translation="ETGEPELDALAREIRRTVKVEALHVDHRKIVTIDGRVAYCGSAN
                     FGAQYQYHVPFDPAKNAHDEASIAREAGQPEPWWKWHDGLVRFEGPIVCDLDRVFRER
                     WRLGGGANFRSLDAEIPAAPRGLRSRGPRTLMMIAAREL"
BASE COUNT          377 a          610 c          621 g          422 t
ORIGIN      
        1 gacgaatatg gcagatagct tgaccggggt tgcggacgct tgctttttca cgttcgagtg
       61 agatggagtc gcggtagtgg ttgacgcctg gccagcgatc gccgggaaac ccactgcgag
      121 aaaagcgacg ccggaaagaa cgaggacagt cattctaaga ctgttgatcg acgtcagctg
      181 aaagggtttc ttcatgatga tggcgctcct ttttcgctct tacgcatcct tcgtgcagga
      241 atggacgcgc tattcgccct tacgagtcaa gatgtgtccg agcagaacgc caaccaggag
      301 cgcgcctccg accacgagcg cagtgttctg gtcggaatat tcgctgatgc gcccggcagc
      361 ttccctaatc tgtctttttc ctcgggctac tttcgtggcg tagtcgtcta cgactccttg
      421 accggccgac ttgaggtcgt cgaatgcctc tgaggcatgg tcaccgaagg tcttcgcggc
      481 ctcgccggca cggtcggcga gacctcccgc ttcgctcttg atgtctgagc caagcctctg
      541 agcgctttcc gagagaggag ctttcccggc atacggtttc gatgaaaagt tttccataac
      601 aactcctttc cgtctctacg ccgcaacatt gctgccatcg cagcgtgcgc ctgcgcagcg
      661 tttcgttcca gcgcgcgcga aagtgcgcga agtgaaaaca gttcttaagc ggcaccgatc
      721 ttgcgcctcc gtccctggag gcgctcatga aagaccgaat ggatggcgac acgaagaaag
      781 ttgaacgtgc cgcgggggca aaccccgacc cgattacagg tgagccaggc tctcatcctg
      841 tcggcactgg tgttggatcg gctgggggcg cggccgcagg agtcgcggtg ggggccgtcg
      901 ttggaggtcc ggtcggtgcc ctagtcggcg gagtggccgg cgcggttatc gggggaggcg
      961 cgggtcatgc cgcaggggaa gctgcgaatc cgactgtcga gaaacagtac tgggagaacg
     1021 tctataaaac tcgcccttat tacaagtcag gtagaacctt tgattattac gagcctgcct
     1081 atcggtacgg gtgggagaac gcgacgaaac cagaattcgc gacgaagagc ttcgatgaaa
     1141 ctgaatctgt cctccatcag cgatggactg cccagcccgg ctcggctact cgcccgtcct
     1201 gggtcgattc caaagaacca gctcgagacg cttggaaccg gacgcgcggc cgcaaggctt
     1261 agcgccgaga aagagggagg tagaaaccgt tcgtcgttct cgcggtcatt cttgcggccg
     1321 cctggttcct cggatttacg atgtttcacg ctgtcggttt cgcgatccac ctactcttgg
     1381 tcgccgccgt cgtctcggtg attgttcact tcatgactgg agggcggact cggagtgtct
     1441 agctaaccac ccgcctagtc gccgatcaac gacagacgtg gtttgaatgc ccgggctgag
     1501 atgaaagccc ggggattcct tttgttgcac ccggaccgtg gcgttcgcca ccatcatgcc
     1561 cgctagcttg gtccgattgc agatgggcag cccggaaacg ctcttacagc tctcgcgctg
     1621 caatcatcat cagcgtgcga gggccccgcg agcgtaggcc gcgcggcgcg gccggaatct
     1681 cggcatcgag cgaccggaag tttgctccgc cgccaagccg ccaacgttcg cggaaaaccc
     1741 tgtccaggtc gcagacgatc ggtccctcga accgaacgag gccgtcgtgc cacttccacc
     1801 aaggctccgg ttgacccgct tcccgcgcga tgctcgcctc gtcgtgcgcg ttcttcgccg
     1861 gatcaaaggg cacgtgatac tggtactgag ccccgaagtt cgcggagccg cagtacgcga
     1921 cgcggccgtc gatggtcaca atcttccggt gatcgacgtg gagcgcttcg accttcacgg
     1981 tcctcctgat ctcgcgcgcg agcgcgtcga gctcgggctc tcccgtctcg
//
LOCUS       DASRKK010000379         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_713572, whole genome shotgun sequence.
ACCESSION   DASRKK010000379 DASRKK010000000
VERSION     DASRKK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713572"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1058)
                     /locus_tag="VFC15_08455"
     CDS             complement(78..1058)
                     /locus_tag="VFC15_08455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM10227.1"
                     /translation="MDYTRLGRTGMEVSRICLGCMTYGDPNRGAHTWTLPEEQARPLL
                     RRALELGINFFDTANTYSDGTSEEFLGRAIRDFANRDEVVIATKVYFPVRKGLNARGL
                     SRKAIMTGIDASLRRLGMDYVDLYQIHRLDHDTPIEVTLEALHDVVKAGKARYIGASS
                     MYAWQFCKALYLADLHGWTRFATMQDHYNLLHREEEREMLPLCAEEKIAVLPWSPLAR
                     GRLARAWDESTNRLETDEYGKKLYVGANDADRRVVERVGEIAQKRSVPRAQVALAWML
                     QKPVVTAPIVGASKQQHLEDAIAALSLRLSPEEISALEEPYVPHPVVGFA"
     gene            complement(1377..>2247)
                     /locus_tag="VFC15_08460"
     CDS             complement(1377..>2247)
                     /locus_tag="VFC15_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10228.1"
                     /translation="IDPKYRNPKIGGQAPIRVVNEVMSAGDGNHGVQTAAYNLPNDER
                     VVVEKGSKRVMLKNVQQAKFDKTLIPISRLVLGGDEQDRVEFEAFFTHILTHELCHGL
                     GPHQITVNGKPTTPREQLKELYSAIEEAKADVTGLFALQYMLDHAQAMGLAGVLKPNR
                     ASEKRLYATYLASSFRTLRFGLNDAHGKGMAIQVNYLMDKGAFVMRPDETFAVDYAKI
                     KDAVRDLTHDLLTLEAEGNYAGAKKMLDTLGVLRPEFKRALDKLGDIPVDIEPIFVTA
                     EKVAPHTTAPHRQ"
BASE COUNT          405 a          692 c          668 g          482 t
ORIGIN      
        1 ggcccaaaga gcaaatcgcc aggttcaagc agtggatgga cgacggcatg gcgccgtaaa
       61 cagcttgccg gactgaatca agcgaacccc actaccggat gcggaacgta gggttcttcc
      121 aaggcagaaa tttcctccgg gcttaaacgc aacgagagcg cggcgatggc gtcttcgagg
      181 tgctgctgtt tggacgcccc cacgatgggc gcggtgacca ccggcttctg cagcatccag
      241 gccagcgcga cctgtgcgcg cgggacgctt cgcttctgcg cgatctcgcc cacacgctcc
      301 accacgcgcc ggtccgcatc gttcgcacca acgtaaagct tcttcccgta ctcatcggtt
      361 tccagccggt tggtgctttc atcccaggcg cgtgccaagc gtcctcgcgc cagcgggctc
      421 cacggcagca cggcaatctt ttcttccgcg cacagcggca acatctcacg ctcttcttct
      481 cgatgtagca ggttgtagtg gtcttgcatg gtggcgaagc gggtccagcc gtgtagatcg
      541 gccaggtaga gcgccttgca gaactgccaa gcgtacatgg acgatgcgcc gatgtagcgt
      601 gccttgccag ccttgaccac gtcgtgcagc gcttccagcg tcacctcgat gggcgtgtcg
      661 tggtccaagc ggtggatctg gtagagatca acgtaatcca tgcccagccg ccgcaggctg
      721 gcgtcgatgc ccgtcatgat cgctttgcgc gagaggccgc gcgcattgag gcccttgcgg
      781 actgggaagt aaaccttggt ggcgatgacc acttcgtcgc gattggcgaa gtcgcgaatc
      841 gcgcggccga gaaactcctc gctggtgcca tcggagtacg tgttggccgt atcgaagaag
      901 ttgatgccca gctcaagcgc gcggcgaagg agcgggcgcg cctgttcctc cggcagcgtc
      961 caggtgtgcg cgccgcggtt gggatcgccg taggtcatgc atcccaggca aatgcgcgat
     1021 acttccattc cagttcggcc cagtcgtgtg tagtccatgt gctctgttcc tcacgaaatg
     1081 tcattgaaag ggcggtttcg caggctgaaa aagtcttttc gaacggaagg ttcgggtagt
     1141 gcggcgcttc ggcgccgcgt caacgagctc atgagaaagc ggcctttagg ccctgaggta
     1201 catgccccga tgtcgctagg atgctcgtat ttcgcggggt ttgaaacgaa gtacctcagc
     1261 ggctaaagcc ggctttcatc gattccgctc aacgcgggcc tgaaggcccg cactacccga
     1321 gcagtattga aaattcttcc gcagtctgtt cagccgtatt ctttcaaaca acttgttcac
     1381 tgccggtgcg gcgccgtggt gtgcggcgcg accttctccg cggtcacgaa gatgggctcg
     1441 atgtccaccg gaatgtcccc caacttgtcg agcgcccgct tgaactcggg acgcagcact
     1501 cccaaggtgt cgagcatctt cttagcgccg gcgtaatttc cttccgcctc cagcgtcagc
     1561 aggtcatgcg tcaggtcgcg caccgcgtct ttgatcttcg cgtaatccac ggcgaaggtt
     1621 tcatcgggac gcatcacgaa cgcgcctttg tccatcaggt aattcacctg gatagccatg
     1681 ccctttccgt gcgcgtcgtt cagtccaaag cgcaaggtgc gaaacgacga tgcaagatac
     1741 gtggcataca agcgcttctc ggaagcgcgg ttcggcttca gaacaccagc cagtcccatg
     1801 gcctgcgcgt gatcgagcat gtactgcaac gcgaacagtc ccgtaacgtc ggccttggct
     1861 tcctcgatgg cgctgtagag ttccttcaac tgctcgcgcg gcgtagtcgg cttgccattc
     1921 accgtgatct ggtgcggccc cagtccatgg caaagctcgt gggtcaggat gtgggtgaag
     1981 aaggcctcga actcgactct gtcctgctcg tcgccgccca gcaccagcct ggagatcggg
     2041 ataagcgtct tgtcgaactt tgcctgctgc acattcttca gcatgacccg cttgctgccc
     2101 ttctccacga ccacgcgctc gtcgttgggc aggttgtagg cggcggtctg cacgccgtgg
     2161 ttgccatcgc ctgcgctcat cacttcattg accacgcgga tgggcgcttg tccgccgatc
     2221 ttcggattgc gatatttcgg atcgatc
//
LOCUS       DASRKK010000380         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1299578, whole genome shotgun sequence.
ACCESSION   DASRKK010000380 DASRKK010000000
VERSION     DASRKK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299578"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFC15_08465"
     CDS             <1..544
                     /locus_tag="VFC15_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZM10229.1"
                     /translation="LVAADLIIMSKVDLIDEAQLGGVRELVREIAPRAKVVEAIRGDL
                     PADLLLGIGETRVAASSPFEVQHGHDPPATRQEHDDLFARWSFVSGRPFDRERLLTTV
                     ESLPPSVLRAKGLLYVAGDPAHSAVLQLVGRRWTLEVGSPWSGADRRTRLVVIGPSGS
                     LDTAQLTEDFSFALADDLRS"
     gene            1515..2822
                     /locus_tag="VFC15_08470"
     CDS             1515..2822
                     /locus_tag="VFC15_08470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZM10230.1"
                     /translation="MRTVILLLIVLVIVLPIAFVLARSATPVVELASPVTVLGQATLI
                     TAHVRDPHGVRRLAAFVEQNGARYPVWEAAQPSTEADSTWNFTAGVKITPQLRDGKAK
                     LILEATSNDLLRKTGRWEREMAVVTQPPSVTADSDQHYLYLGMADLATFTVSGNWTEA
                     GVRVGDEKFRAWPMPGGKPGLFSLYAFAWNMPSGTVPLVYASNGAGSDVTSPLVVEFP
                     KKEQPKHNTHDLQVSDAFLQKVVGELDPNGSSDPVVRFVRINSEMRRANNQTLSDLRF
                     KTADRFLWSRPFIRQPHSQAEATFADVRNYIYHGKKIDQQVHLGYDLAVTQHVGVQAS
                     NDGKVVYAAPLGIYGNCIVVDHGYGLQTIYGHLSKIDVHEGDMVNQGEVMGQSGQTGM
                     AGGDHIHFAMQLDGVQIDPKEWWDPHWIKDHIAKRVELHGFSK"
BASE COUNT          665 a          855 c          930 g          607 t
ORIGIN      
        1 gctggtcgcc gcggacctga tcataatgag caaggtcgac ctgatcgacg aagctcaact
       61 ggggggagtg cgcgagttag tccgcgagat agcgccacga gcgaaagtcg tcgaggccat
      121 tcgcggggat ctccccgcag atttgctgct cggtataggc gaaacgcgag tggcggcatc
      181 gtcgccattc gaggtgcagc acgggcacga tccccctgct acacgccagg aacacgatga
      241 tctgtttgcc aggtggagct tcgtgagcgg gcggccgttc gatcgggagc gcctgctgac
      301 cacagttgaa tcgctgcctc catccgtact gcgggccaag ggcctgctct acgtggccgg
      361 ggatcccgct cattctgccg tcttgcagtt agtcggccga cgctggacac tggaagtggg
      421 aagcccctgg agcggagcgg atcgacgcac gcgcctggtc gtgatcggcc cgtctgggag
      481 tctcgacacg gcgcaactca cggaagactt cagtttcgca ttagcggacg atcttcggag
      541 ctaagcaacc cggagctgtg cgctacatta ttgtcgagag agcagcctcc agagttagga
      601 agagtggtgg atgtgcaagg ttttgacgac ccacaggtgg tagtcaaccc ctggctgaga
      661 agtatttgga ggccacccag caggaaccca aaaacagcgg ctgcggttgt cggccgccga
      721 tcatctcctc tgggtctgac tgtcccgtat ttgggcggaa tggagatcga cccttcgtat
      781 cgtgcagcgc gagacggtca tcgccctagc atcgcaaagg ctttcggttg tactggtctt
      841 ggaagagtcg cgctgatcaa ggtcgcccgc cagtctcgat ggacgtgcgg gaactgattc
      901 gacgacgtgc tgggggcggg gttggcggcg gcagaaacct ggaaaccgtt gtttccgata
      961 gatcaaagcc cagcatgagc aactcaccgt gaatgcgtgg cgctccccac gttggattct
     1021 cagcaaccat ttgaaaaatc aaggctcgaa tttctttgct cacaggcttg cgccctcccc
     1081 tcgaccggcc cttgaaagcc atttccaata cagccgaaag ccggcacgat gccagccgag
     1141 aaccgtttcc gggcgtgacc aggatgagag gttctttcca tcgagcccat agcctacgca
     1201 gtacgaccca gaacagcgtc tgcgaagtgg tgagtcgcgg gcgaggccgt tgggcgtgga
     1261 gagccagcaa ctgctgacgc agagcagagt tttcgaggat gagatcctgg cgggcacaaa
     1321 aggcactgcg aagccagccg aggaagtgcc ggagccacac gatcctggct agaatcttat
     1381 ccgacgttgc caatccggcc atcgcataag tcattgctat tggcgatgta cgatgttttg
     1441 ctgaatgaca ggggttactg aactcggtaa caaacaccag atcgccagcc tcctgacatt
     1501 cgctacaatg atttgtgcga acagtcatcc ttctcctcat tgtgctcgtc atcgtgttgc
     1561 cgatcgcgtt tgtgctggcg cgatcagcaa cgcccgttgt agagctggca tctccagtca
     1621 ctgtcctcgg ccaagctact ctcattaccg cacatgtacg cgatccgcat ggtgtccgca
     1681 ggctcgctgc atttgtcgag cagaacggcg cccgttatcc cgtttgggaa gcagcacaac
     1741 cctccaccga ggcggacagt acatggaact tcacggcagg agtgaagatc acaccgcagc
     1801 tgcgggacgg caaggccaaa ttgatccttg aagcaacctc caacgacttg cttcggaaaa
     1861 ccggccgctg ggaacgcgaa atggcggtgg tgacgcaacc gccaagtgta accgccgatt
     1921 cagaccagca ctacctgtac ttgggcatgg ctgacctggc gacgttcact gtctcaggca
     1981 actggacaga ggctggtgtg cgtgtcggcg acgagaagtt tcgcgcgtgg cctatgccgg
     2041 gcggcaagcc gggcctgttt tccctctacg cctttgcctg gaacatgcca tcgggaaccg
     2101 tgccgctggt ttacgcctcc aacggagccg gaagcgacgt gaccagcccg ctagtcgtcg
     2161 agtttccaaa gaaagaacag ccgaaacaca acacgcatga cttgcaggtg agcgacgcgt
     2221 ttctgcagaa ggtggttggc gagcttgacc cgaatggctc cagtgatccg gtggtgcggt
     2281 tcgtaaggat caatagcgaa atgcgtcgag ctaataacca gacactttcc gatctgcgct
     2341 tcaaaaccgc ggacaggttc ttgtggtcgc ggcctttcat caggcagccg cattcccagg
     2401 ccgaagcgac ctttgcggac gtgagaaact acatttatca cggaaagaag atcgatcagc
     2461 aggttcacct cggatacgat ctggcggtga cgcagcacgt cggcgtgcag gcgtccaacg
     2521 atggaaaggt cgtctatgcg gctccgctcg gcatctacgg caactgcatt gtcgtcgacc
     2581 atggctacgg cctgcagact atctacggcc acctcagcaa aatcgatgtg cacgaaggcg
     2641 acatggtgaa tcaaggcgag gtcatgggac agagcggcca gaccggcatg gccggcggcg
     2701 accatattca cttcgccatg caactcgacg gcgtccagat tgacccgaag gaatggtggg
     2761 acccgcactg gattaaggat cacatcgcga aacgagtcga actccacggg ttcagcaaat
     2821 gatagataag cgggtgtgct ggccaggtga cccatgagga gtttgttccg ctgcgtccag
     2881 caggttttgt tcgttgtgct gttccgtgcg gttggcctag cagcccgtgg tgcaagtctg
     2941 gattcgtaag caaaaacatc tgaggtatcc acagacattg tttgtagaat cgccctatgg
     3001 cgatgggcac gcgcaagcag cgggagaagc aagaggggtt gtggatcgcg cacacag
//
LOCUS       DASRKK010000381         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1202279, whole genome shotgun sequence.
ACCESSION   DASRKK010000381 DASRKK010000000
VERSION     DASRKK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202279"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFC15_08475"
     CDS             <1..466
                     /locus_tag="VFC15_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM10231.1"
                     /translation="IGVLTSLSRDGEYIARVIRRFGFVPVRGSSSRGGQRGLLEMETM
                     VKAGGAVAFTIDGPRGPRYVAKKGPVTLARLTGIPITAFYVAVEKPWVLKTWDAMLIP
                     KPFSRAYVRVAKNTFVPPDADDAALERYHAEMQASLERITSFAESQFSARHD"
     regulatory      534..728
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            800..>2001
                     /locus_tag="VFC15_08480"
     CDS             800..>2001
                     /locus_tag="VFC15_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZM10232.1"
                     /translation="MSSVRLLTIVCGFLIALLCCTAALASDFKVSVESPAGGRVSGVQ
                     VSLFRIGDNAGVGVQTTAGDGTATFPHLAAGQYRLVVLAPGFAEQSLQVSIPQTETLS
                     VQLKLATTPQTVVVSATATPATPEQTGTSVGVLTGEQLNAINPVSAPDALLYIPGAIV
                     SSTGRRGNLASLFVRGGESNYNKVLVDGVPVNDPGGIFDFGVVPMNNMDRLEVERGAE
                     STVYGSDAMTSVVQLWTATGSTRTPEIQFGADGGNFSTANGYASLAGARGIFDYNVFA
                     NQFNTQGQGVNDAYSNSLQGGNVGVRLSDRVAFRLRARHYNSWTGIQSNWWFNGNPEL
                     PPDPNQYAHQNNFLASGDLTISGPGAWQHQFSGFEYHHVALNVNPLDDLDRPFDSAFN
                     SLAKYNLAG"
BASE COUNT          383 a          636 c          595 g          387 t
ORIGIN      
        1 tatcggagta ctgaccagcc tcagccgcga cggcgaatat attgcgcgcg tgattcgccg
       61 cttcgggttt gtgccagttc gcggctccag ttcgcgcgga ggccagcgcg gcctgctgga
      121 aatggagacc atggtaaaag cggggggcgc cgtagctttt accattgatg gtccccgcgg
      181 tccgcgctac gtcgccaaaa aaggcccggt gacgctggcg cgcctaaccg gcatccccat
      241 cactgcgttc tacgtcgccg tggagaagcc ctgggtcttg aagacgtggg acgccatgct
      301 cattcccaaa ccgttttcgc gcgcctatgt gcgggtggcg aaaaacacct tcgtaccgcc
      361 cgatgccgat gatgcggcgt tggagcgcta tcacgccgag atgcaagcct cgctcgagcg
      421 catcaccagc ttcgcagaat cacagttttc cgcacgtcac gattgacact tttctcccgc
      481 gtcagtacag tcgaatctca tcctgtgaac caactcggtc cgggattgtt ccttgccaat
      541 tcgtgttcgc gcgttccgtc gtctcaccgg cggaactggg aagcgggtgt gaagcccgcg
      601 ctgccgcgca actgtaagcg agtctttggt ggatcggcca ctgttccaac gggaaggccc
      661 ccgccgtcgt ccgcgatgca aatcgcaact cgcaagccag gagaccggcg cgaactacgg
      721 ggtcccggcg aacgccgact ttgcgtgcga tgggggtgcc ctgatcacca acccctttcg
      781 cgtgtaaagg aagagtgtca tgtccagcgt tcgtctcttg accattgttt gtggatttct
      841 catagcgctg ctgtgttgca cagccgccct cgcatccgat ttcaaagtgt cagtggaaag
      901 ccctgccggc gggcgcgtct ccggtgtgca ggtcagcttg tttcgcatcg gcgacaacgc
      961 cggcgtagga gtgcaaacca cagccggcga tggtacagca acgtttccgc atctcgccgc
     1021 cgggcagtat cggctggtag tgctcgcacc gggattcgcc gagcaatcgc tgcaagtttc
     1081 tattccgcag acggaaaccc tcagcgttca actgaagctt gcgaccaccc cgcaaaccgt
     1141 ggtcgtctcc gccaccgcaa ctcccgctac gccggagcag acgggcactt cggtcggggt
     1201 gctgaccggt gagcaactga acgcgatcaa tcccgtctcc gccccggatg ccttgctcta
     1261 tattcccgga gccatcgtca gcagcactgg gcggcgcggc aatctcgcca gcttgttcgt
     1321 tcgcggcggg gaatccaatt acaacaaggt gctggtggat ggcgtgccgg tgaacgatcc
     1381 cggcggcatc ttcgatttcg gcgtggttcc catgaacaac atggaccggc tcgaagtgga
     1441 acgcggcgcc gaaagcaccg tctacggatc ggacgccatg accagcgtgg tgcagctttg
     1501 gaccgcgacc ggcagcacgc gcacgccgga gattcaattc ggcgccgacg gcggaaattt
     1561 ctccaccgcc aacggctatg cgtcgctggc gggagcgcgc ggaatcttcg actacaacgt
     1621 cttcgccaac cagttcaaca cgcaaggcca gggagtgaac gacgcgtatt cgaactcgct
     1681 gcaaggcgga aatgtgggcg tgcgactttc tgaccgcgtg gcatttcgcc tgcgcgcacg
     1741 tcactacaac agttggacag gcattcagtc gaactggtgg ttcaacggga atcccgagct
     1801 gccgccggac ccgaaccaat acgcgcacca gaataatttc ctggccagcg gcgatcttac
     1861 gatcagcggg ccgggcgcgt ggcagcacca gttcagcggc ttcgagtacc accacgtcgc
     1921 gcttaacgtg aatcccctcg acgatcttga ccggccattc gacagcgcct tcaacagcct
     1981 ggccaagtac aacctcgccg g
//
LOCUS       DASRKK010000382         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1299732, whole genome shotgun sequence.
ACCESSION   DASRKK010000382 DASRKK010000000
VERSION     DASRKK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299732"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..899)
                     /locus_tag="VFC15_08485"
     CDS             complement(450..899)
                     /locus_tag="VFC15_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10233.1"
                     /translation="MNPLRLHHIGYVVASIDVAMPGFVRSLGASWNGQVFHDPNQKVK
                     VAFLTTRAEDARVELVEPVGDDSPVLRFLREKGGGLHHACYEVADLENQLSEFRSRGA
                     VIAKRPKPAVAFDGRRIAWIITAERFLIELLEQSASSVSPPGGIVRG"
     gene            complement(902..1144)
                     /locus_tag="VFC15_08490"
     CDS             complement(902..1144)
                     /locus_tag="VFC15_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZM10234.1"
                     /translation="MDDTRKRLTSCFQVVFPDLTEAEIPVASQERVAAWDSVAGITLV
                     SVIEDEFGLEMDLDDLDNLDSFEKVYAYLQKRLKVA"
     gene            complement(1137..2729)
                     /locus_tag="VFC15_08495"
     CDS             complement(1137..2729)
                     /locus_tag="VFC15_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HZM10235.1"
                     /translation="MVTPGLYGSLAETLEGTSDSAIDGVAIALEWSDLDARLDFRSAG
                     SWGRSAATDIVANAQAMLDRVAAAISRVPSGVRVALALPTLPLPPLFHAPGWQASEHE
                     LHLESEIFRFAAAMTQSARATVINVRRLLETSPPGSSFDLKSHLHTGLPYTTPHADRV
                     ASQLALALVPPVPKKGIISDLDDTLWSGIAGEIGPENVSWDIASHRHLHGLYQKLLAS
                     LSEHGTLVGIASKNDPKVVARAFEREDLLLRLEQVFPVEVHWNAKSGSVSRILETWNI
                     AADSVIFVDDSPMELAEVAAAHPGIECVLFPKDDYAAGLAMLRRLRDACGKERVSSDD
                     ALRLESIRQGAAFRVQAAAAATPESFLAQAEAVLTFDFGCAAEPRALELVNKTNQFNL
                     NGRRYTEADWRNLTSQPDAVVVSVSYEDRFGPLGTIAVVAGVVHGKQLAIETWVMSCR
                     AFARRIEQQTLKMLFESTGAEEVDFDFVPTAKNSPLQEFFAAVTGEQPTAEFRMRRAQ
                     FDESCPALYHAVRELRRAEAHG"
     gene            3610..4224
                     /locus_tag="VFC15_08500"
     CDS             3610..4224
                     /locus_tag="VFC15_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM10236.1"
                     /translation="MNTALYARVSTSSKGQDPEMQLTELREFASKRGWQIVDEFVDIG
                     VSGSKDHRPQLDAMMRLAQARKVDVIVCWKLDRFGRSLRHLVDALAELQAVGCAFVSL
                     RDNLDLTTPAGRMMFHVIGAMAEFERELIREQVKAGLAHARSKGRIGGRPKVKRERDK
                     DAKIIREMRADGDSYGEIAEELGRSKGDIYRVCMTLGCSPGMGA"
     gene            4221..4439
                     /locus_tag="VFC15_08505"
     CDS             4221..4439
                     /locus_tag="VFC15_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10237.1"
                     /translation="MTGKKSRMLMVRSVVLAMEDFARLQAGEPVQGKRRSSRKAGEVL
                     LLSISYAKQQTKDETGIGRATSRKRAAL"
BASE COUNT         1069 a         1334 c         1401 g         1024 t
ORIGIN      
        1 cgttggtttg cccatatacg acctgatcgt aattgacatc gtcaggaagc ggaagggcct
       61 ccctaaacgc agatttgagc ttatctaaag agttttgcac cgtcgtaaca gaaggccttt
      121 cggaggattt tatctgttgt ctcaaaaaag actggtgaac tttggctacc agttcgtccg
      181 ctgaagccag aataccatct tctcaagagt ttgccatctc cggtaaccaa tcggtaggct
      241 gggcggggcc agcaagtctt gtttgggaac gtagcgacgg aaggttcttg acatgatcgg
      301 caaactatac tgagcagcaa atccgaacct cagcagtggg ttgaaaaatc tgggtagagg
      361 cagagtactc gacgcgaagc gacgagcggt atcgcgcagc caacgataga aaggggtctc
      421 gccccgccgg attttgagca tgaccgaact catccgcgga cgattcctcc gggtggcgaa
      481 acggagctgg ccgattgctc gagtaattcg atcagaaacc tttcggctgt gatgatccag
      541 gcgatgcgtc gtccgtcgaa ggcgaccgcc ggcttgggcc gcttcgcgat aacagcgccg
      601 cgcgagcgaa actccgacaa ctggttctct aggtccgcta cttcgtagca ggcatggtga
      661 aggccgccac ccttttcgcg cagaaaccgc agcactggcg aatcgtcgcc caccggttcc
      721 accaattcca cgcgcgcatc ttccgcccgg gtcgtgagaa aagccacttt gaccttctgg
      781 ttgggatcgt ggaatacctg tccattccag ctagccccta gcgaacgcac gaagccgggc
      841 atggcgacat cgatgctggc gaccacatag ccgatatggt gcaggcgcaa cggattcaaa
      901 atcaggccac cttcagccgt ttctgaagat aggcgtagac cttctcgaag gagtccaaat
      961 tgtccaaatc gtctaaatcc atttccaagc cgaactcgtc ctcaatcacg ctcaccaggg
     1021 taatcccggc aaccgagtcc caggcggcga ccctctcctg cgaggctacc ggaatttcgg
     1081 cttccgtcaa atccggaaac acgacttgga agcaactggt cagtcgcttt ctcgtatcat
     1141 ccatgggctt ctgctctcct caactcgcgc actgcgtgat agagcgcggg acacgactca
     1201 tcgaattgtg cgcgcctcat gcggaactcc gccgtaggtt gctcgccggt aaccgctgca
     1261 aaaaactcct gtagagggct gttcttggcc gtgggcacaa agtcgaaatc aacttcctcg
     1321 gcgcctgtgc tctcaaacaa catcttaagc gtttgctgtt cgatacgacg cgcaaaagcg
     1381 cgacaactca tcacccaagt ttcgatcgca agttgttttc cgtggaccac tccggcgact
     1441 acggcgatgg ttcccagagg cccgaatctg tcttcgtagc ttaccgagac gaccaccgcg
     1501 tcaggctgag acgtcaagtt gcgccagtcc gcctcggtgt accgtcttcc attcagattg
     1561 aattggtttg tcttgtttac taattccaga gcccgaggct cggcagcaca gccaaaatca
     1621 aaggtcaata ccgcctccgc ctgggccagg aagctttccg gagtggccgc tgcggcggcc
     1681 tgtacccgga acgcagcgcc ttggcggata ctctcaagtc ggagggcatc gtcggaggaa
     1741 acccgctcct tgccacatgc atcgcgcaac cgccgcaaca tcgccaagcc ggccgcgtag
     1801 tcgtccttgg gaaacagaac acattcaatg ccggggtggg cagccgctac ctcggccagt
     1861 tccatcgggc tgtcatccac aaagatgacg ctgtcggccg cgatgttcca cgtctccaat
     1921 atccgcgata cgctccccga ctttgcgttc cagtgcactt cgacagggaa tacttgttct
     1981 aagcgaagca gaagatcttc cctctcgaat gcccgggcca ctaccttggg atcgtttttg
     2041 ctggcaatac caaccagggt gccatgttca gagagcgaag ccagcagttt ctggtacaac
     2101 ccatgtagat ggcggtggct ggcgatgtcc cacgacacat tttccgggcc tatttccccg
     2161 gcgattccgc tccaaagagt gtcgtctagg tcggaaatga tgcctttctt aggaacaggg
     2221 ggaaccagcg ccaatgccag ttgagacgcg actcgatcgg catgcggcgt ggtgtagggc
     2281 aagccagtgt gcaggtgact tttcagatca aagctagaac ccggtggcga cgtttcaagt
     2341 aaccgccgca cattaattac agttgcgcgc gcagattggg tcatagccgc tgcaaagcgg
     2401 aatatctcac tttcgaggtg cagttcgtgt tccgaagcct gccatcccgg cgcgtggaac
     2461 agcggcggca acgggagagt tggaagcgcc agcgcgaccc gtacgccgga agggacgcga
     2521 gagatcgcgg cggcaacgcg gtccagcatg gcctgcgcat tggctacgat gtcggtggct
     2581 gcgctccgtc cccaactgcc ggcgctgcgg aagtcgagtc tcgcgtccaa atcggaccat
     2641 tccagcgcga tcgcgacacc gtcaatggcc gaatcgctgg ttccctccaa cgtttcggca
     2701 aggcttccgt ataggcctgg cgtgaccacc accttgcgat tggccagcga ctgctgcagg
     2761 tgggccgaca gaaacgtttg caggtgaaga ggcgtaaagc ctcaggccag aaccacggaa
     2821 aacgggcgcg cattcgctgg cgcgctctgg gtgattttga gtgcctcggt aatcttcaca
     2881 aaatctttac cttgccggac aaaatggcgg ttgcagactc atggtggcaa gctaaccgcc
     2941 agaaacttat ccaaatgact atatgcaact gactaaggcc cacgcaaggc gcgcaggtgg
     3001 ttggtatcgt cttctctcaa atcaatctgt acggtccctt tctcacggcg gttagaccgt
     3061 ggcctggttt caatcgcacg gaccggatca ctgaatggcc cggattatag caggtgtcca
     3121 ttgtgtccat tttgtagggc gggctcgctc gctcgcgcgc gtactggttg aaaaactccc
     3181 gatttgaata gcgtcctgta cgcgccgggt ggccgctgtt ggcgtcttgg cgtctcggtg
     3241 atgctaggga gacggcggaa cgctctcagg ggcgcgtagg gcgttctctg agcttcttgg
     3301 ttagacgaaa atcggcggtg ggtgtgcata accagaggaa gtctgctctt tttttaggcg
     3361 tccatttcgt acatttgttt gcggggtcat gtcggttggt tgtgggtgtt cacgagattt
     3421 ctcggcgctg gagcttgccg gccccagaat cgttccaaaa aaacccagca aaagcgggag
     3481 ccgccatggg cgtgttttca gcaacttgga ctaacgcaat tactgttcca gtaatcaatt
     3541 gttttctgta cgattgctct tgacttgcga gattagaggg gtaggatgcc tttagtagag
     3601 ggaaacgcaa tgaatacagc actttatgcc cgagtttcca ccagcagtaa gggacaagat
     3661 cccgagatgc agctaaccga actgcgggag ttcgccagca agcgtggctg gcagatcgtg
     3721 gacgagtttg tggatattgg cgttagtggg tccaaggatc accgcccgca actcgacgcc
     3781 atgatgcgtc ttgctcaggc gcgcaaggtg gatgtgattg tttgctggaa actagaccgc
     3841 tttggccgca gtctgcgcca ccttgtggac gccctggccg aactgcaggc ggtaggctgc
     3901 gctttcgtgt cgctgcgtga caacctagac ctgaccacgc cggcggggcg catgatgttc
     3961 catgtcatcg gcgctatggc agagttcgag cgggaactaa ttcgcgaaca ggtgaaggcg
     4021 ggtttggccc atgcgcgcag caagggccgc atcggaggaa gacccaaggt gaaacgcgag
     4081 cgcgacaagg acgccaagat catccgcgag atgcgcgccg atggcgactc ttacggcgag
     4141 attgccgaag agctagggcg ctcgaagggt gacatctacc gggtgtgtat gacgctaggc
     4201 tgctcgccgg ggatgggagc atgacgggga agaaatctag gatgctgatg gtgagaagcg
     4261 ttgttttggc tatggaggac ttcgcaaggc tgcaggccgg tgaacccgtg cagggcaaga
     4321 gacgcagcag ccgcaaggct ggtgaagtac tgctcctctc gataagctat gcgaagcagc
     4381 agacgaaaga tgaaacaggg atcggcagag caactagcag aaaacgagcg gcgctgtaag
     4441 tggagtgcca cactcgccgc actcgcacgg ctcggcttcg aagggcgaat gaacacagac
     4501 aagacctgcg ggagcataga acaattcatc gattcgcact acatacgcaa tcccgcctcg
     4561 tttcccggtc ccggtaaatc gggccgaagt ctattttttc cctactgccc tgtgatttca
     4621 ggtagtgcag ggggcaggag gtggcgtctg gcagaagtga ccttgtatca ttgctcgcag
     4681 atgcgcggaa tcccaaaatc tcaagagaaa ccatgagtgt gctaagggcc ataaaaaact
     4741 cattgctcgc aacaaagggc atgggaacct tctccctctt gtcgaatagc acattccgtc
     4801 gaaaccgctt gctgatattg tgctatca
//
LOCUS       DASRKK010000383        12106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_29544, whole genome shotgun sequence.
ACCESSION   DASRKK010000383 DASRKK010000000
VERSION     DASRKK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29544"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..749
                     /locus_tag="VFC15_08510"
                     /pseudo
     CDS             162..749
                     /locus_tag="VFC15_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(867..1481)
                     /locus_tag="VFC15_08515"
     CDS             complement(867..1481)
                     /locus_tag="VFC15_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM10238.1"
                     /translation="MSSTPDTETLDPRIRRTRQSLQDALEKLLSEKGFEKISVQDVAE
                     AATLNRATFYDHYPDKFALLECLVATRFRQLLEERHIRFDGCKGAVRTMAIGVCSYLA
                     GMPRATLNSQTHSGHHLETAIVAVMRGMILEGLRTHRGRQRVSPGLLASTVAWAIFGA
                     AKEWLRTPNRVPVDRIATTIEAMVNPIFSSAETQSSASKSRLNT"
     gene            1620..1997
                     /locus_tag="VFC15_08520"
     CDS             1620..1997
                     /locus_tag="VFC15_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10239.1"
                     /translation="MKIIATIARYLLGFVFTVFGLNGFLHFLPQGTVPPLAAQFAGAL
                     IQSHYMAVVFALQLICGLMLLANRYVPLALTILGPILFNILLFHIFMAPSGLPLAALV
                     TVLWAVLAYRARSVFAVLFQNSY"
     gene            complement(2008..2424)
                     /locus_tag="VFC15_08525"
     CDS             complement(2008..2424)
                     /locus_tag="VFC15_08525"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZM10240.1"
                     /translation="MKKSDASQVQPASELISKRIAELGDWRGETLSRMRQLIQQADPG
                     VVEEWKLGIPVWSHDGILCTGESYKKVVKLTFAKGASLTDSARLFNSSLDGNVRRAID
                     IHEGEEVDESAFKALVRQAVALNRSGKSKPPKKAKF"
     gene            complement(2943..4085)
                     /locus_tag="VFC15_08530"
     CDS             complement(2943..4085)
                     /locus_tag="VFC15_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10241.1"
                     /translation="MGNHENPFIFGEIIDDENFVNRTAELRQLVRDLADGQKVFLLSP
                     RRFGKSSLVAMALLKLKKRHIRTVSLTVSSYSSYAQFLEKFAEKVLRAAGPWDRVKDW
                     ITRFGRQVKPDVNYNITTGEVSLSLGKGAGFDPAPIAPEIFALPGELTRNGGFRMAIC
                     LDEFQQITQFNGGSVENVIRNQVQEQRGVGYVFAGSQPSLMQEMLTASRPFHKAGPQM
                     FLDKIAAADWKEFITAQFHKRGRTLDEKAIDALLTAADLIPYDVQRIAHELWDTAELQ
                     KKPRLTVADVDSVIGALVSGQSTYYELLWEQLAARQRAALQAMAARGPSEIYSQAVRE
                     EFRLGPASTVQKALQSLDSRDILDRYHGNYFFLDPLLPLWIKKKGA"
     gene            complement(4207..4410)
                     /locus_tag="VFC15_08535"
     CDS             complement(4207..4410)
                     /locus_tag="VFC15_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10242.1"
                     /translation="MKEKAPQTERNLLQVRLSDNEKRNIKMMAAGQGLTLREATLAAF
                     TAWAEKLRAQAAAAKAAKAAGDR"
     gene            complement(5009..5377)
                     /locus_tag="VFC15_08540"
     CDS             complement(5009..5377)
                     /locus_tag="VFC15_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZM10243.1"
                     /translation="MTATMERTPAVLSATTLTGDKVVNFQKEDLGKIEHLMIDLESGR
                     IAYAVLSFGGFLGMGDKLFAIPWSALSVDTVEHRFILNVDKGLLKAAPGFDKEHWPDM
                     ADRVWGAKVYTYYGAKPYWN"
     gene            5738..6067
                     /locus_tag="VFC15_08545"
     CDS             5738..6067
                     /locus_tag="VFC15_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM10244.1"
                     /translation="MKSEKLEVVRGSGNAFRDLGHKSADAEQFKAILAAEIIKALDRE
                     RLTVRAAHARTGIAAADFSRIRSADLGRFTVDRLISVLNRLGSRVEVKVRVHRATGSV
                     QEARVGA"
     gene            6167..6754
                     /locus_tag="VFC15_08550"
     CDS             6167..6754
                     /locus_tag="VFC15_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10245.1"
                     /translation="MNWKNPRSVEATITRLTREIAKIDEVFYMVNETDDRVLYAGMLE
                     RKRDDVVRAAVLQMHTAIEDLLNSQIICRILNVKPQDRARKMRSKLAQALRSMLVGPR
                     SLGFEMKLNFAVVLGIVRTGTKDRLTELNTLRNKCSHNWLLKAPVRRGKRPKQKKSPL
                     LLYRGRDLYNVDVLAEFLAEYGPIYYKLFGRYLDL"
     gene            6788..7288
                     /locus_tag="VFC15_08555"
     CDS             6788..7288
                     /locus_tag="VFC15_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10246.1"
                     /translation="MGHPLKDFSRVYERGAGHPPRNESFNTALAKEAQMAASRLLIVE
                     SINDDLTKRRIASKQAKFAADDLSVEYCYALESTRRIDKQSLLDYIDRKINSFRPDLM
                     IVHAGFVFSQHPETFLSVLTELSSKHPLLRIGSQMSEIEPGWKSRFALVFEDTKEIRD
                     LAMRLV"
     gene            complement(7316..7900)
                     /locus_tag="VFC15_08560"
     CDS             complement(7316..7900)
                     /locus_tag="VFC15_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZM10247.1"
                     /translation="MSEEELERAIVSVINSDDLVRVQFMEIFHEKRLRKMLTFDDYKR
                     FCHASLIAASIKSSRVFISYAAKDQKIAEELREALTRRGIIAFMASRDVKGGDLWEPS
                     IREAIVACSEMLVVITPNSKDRPWIMIEVGAGWALGKHVTPCLAFVDVSEVPEALSRS
                     QARSILTKKDRDALIKELSNRLGISKKSQVASRS"
     gene            complement(8486..9883)
                     /gene="gdhA"
                     /locus_tag="VFC15_08565"
     CDS             complement(8486..9883)
                     /gene="gdhA"
                     /locus_tag="VFC15_08565"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HZM10248.1"
                     /translation="MTVGIMQPGIKTPGDYINEVMGLVKAKNPAEPEFHQAVQEVMES
                     LRLVLERHPEYISERILERITEPERVLMFRVPWFDDHGNIQVNRGFRIEMNSAIGPYK
                     GGLRFHPSVNLGILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSF
                     MTELSRHIGPDTDVPAGDIGVGGREIGFLFGQYKRLRNEFAGVLTGKGLAWGGSLIRP
                     EATGYGCVYFADEMLKSRKDTLEGKLCLVSGSGNVSQYTVEKLLDFGAKPVTVSDSDG
                     VVYDPDGIDREKLAFVMDLKNVRRGRIRDYTDQYKKAIFMPSDVKLGYNPLWNIKADC
                     AFPSATQNEINVKDATNLINNGCKLVAEGANMPSTLEATKLFLEAGLLYGPAKAANAG
                     GVATSGLEMSQNSMRLTWTREEVDDRLHKIMIAIHKNCYETAEMYGTPGNLVNGANIG
                     GFLKVANAMLDQGLV"
     gene            10505..11992
                     /locus_tag="VFC15_08570"
     CDS             10505..11992
                     /locus_tag="VFC15_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZM10249.1"
                     /translation="MASAPLQALHRGLFEGYDTGAFFDEMFVAPGVPRPHYAKLFQML
                     GSIPQTQFEERRNLADIAFLLQGITFTVYNDGGTGTERLFPFDLIPRILPHSEWIHIE
                     RGLSQRVVALNLFLQDIYGQQRILKERKIPRSLIYSCHHFHREMIGVEVAHGIYTHIC
                     GIDLVRDSKTGDFLVLEDNVRTPSGVSYVLENRQVMTRTFPDAFQSYEVLPVHDYPTE
                     LCRILRTLSPRGEDNAQIVLLTPGIYNSAYFEHSFLAQQMGIELVEGRDLIVDAGVVY
                     MKTIHGLQRVDVIYRRVDDEYLDPLVFRPESLMGVPGLMGAYRAGNVALANAVGNGVA
                     DDKAVCAYVPDFIRFYLGEEPILGTVETYLCSRPDDLRYTLAHLPELVVKAVGESGGY
                     GMLIGPASDEETIEDFRKKVEADPRNYIAQPVIGLSRVPSYDVESKRMAGRHVDLRPY
                     CLYDGQGVTIIPGGLTRVALKPGSLVVNSSQGGGSKDTWVLREDS"
BASE COUNT         2542 a         3284 c         3580 g         2700 t
ORIGIN      
        1 aaacgcaatg aggtcgagag gatgtttcgt cgactgaagg gcttccgtcg catcttctcg
       61 cgcttcgaga aactcgatgt gatcttcgtg ggcttcatcc acttcgcgct gatcgtggaa
      121 gcactccgat tcctgtttag tgttaacacg ctctagacca gggaatagca ttcgtcgaga
      181 agcacaccgg cgtgcgtcca gcgaatggcg gtgtgcatcc gggacgaggc acgcgcaacg
      241 cgctgctcgg actggggcag gggcgttatc tcgaagtgat tgcgcccgat ccggcgcaga
      301 aagaagttcc taaagtaagg gctgagttgc cggcaatgtt gaagcaactc gcgattccca
      361 gacttgtcgg ctgggcggtg cgcaccttga atatcgaagc ctcggccgag cggctgcgga
      421 aatatggtgt tgcgtttcag ggaccgacgc cggggtcacg cgcgcgtccc gacggtcgtg
      481 tgctgcattg gaagaccttg aacttcgacg acgatcgcga cggggtactt cccttcctga
      541 tcgagtgggg cgcggactca ggacacccgt ccgctgatgc gccggcgggt tgcaatctgg
      601 agggcttcgc cgtggtcagc accgatagca gtgcattgtc cgcggagttt caggagttcg
      661 gcatcgacgt tgtggtccag agcggcaagg cggcacacct gtacgcaaag atcgttggcc
      721 cgaaggggac gatggtcctg ggcgagtgat gcgaaacccc gcatcgtgac ggtcccctcc
      781 ccaaaaagct ccttggattc atgggtttaa cgggattttt tcggcaacga atagggggag
      841 gaggtgagcc catcggccgt ctgatatcag gtattcagtc ggctcttgct ggcgctggac
      901 tgggtctcgg cggagctgaa aatgggattg accatggcct caatggtggt tgcaattcga
      961 tcaaccggca ctcgatttgg cgtgcgtagc cattctttgg ctgctccaaa gatggcccat
     1021 gcgaccgtag atgccaggag tcccggcgaa actcgttggc ggccgcgatg agttctaagt
     1081 ccttccagga tcatgccgcg catgaccgcg acgatcgctg tttctaaatg gtggcctgag
     1141 tgtgtttgcg aattgagcgt ggcccgaggc atcccggcga ggtagctgca aaccccgatg
     1201 gccatcgtcc gtactgcgcc tttacagcca tcaaaccgta tgtggcgctc ctccagcaat
     1261 tggcggaaac gggtggcaac caggcattcc agcaatgcga acttgtcggg gtaatggtcg
     1321 taaaaggtgg ccctattcag ggttgcagcc tcggcgacgt cctggaccga aatcttttcg
     1381 aagccctttt cggagagcag cttctcaagg gcgtcctgca gcgattggcg ggtgcgcctg
     1441 atgcggggat ccagtgtttc tgtgtctgga gtagaggaca taagcccttt cctgcacgat
     1501 agcgattatt ttgcatcgac gacagctgtc gttcatctat tcgacagagg ttgttttaac
     1561 atcatctgtt gacaaaagca aaatcgcatg gctcccagtc cacttttgag gagaatccaa
     1621 tgaagatcat cgcgaccatt gcccgctacc tgttggggtt cgttttcacc gtattcgggt
     1681 tgaacggttt tctgcacttc cttccgcaag ggacggttcc gcccctggcg gcgcaattcg
     1741 caggcgcctt aattcaatct cactacatgg cagtggtgtt tgcgctgcaa ctcatctgcg
     1801 ggctgatgct gttggcgaac cgctatgtgc cgttagcgct aacgatcctt gggccgatcc
     1861 ttttcaacat ccttttgttt cacatcttca tggctcctag cggattgccg ctggccgctc
     1921 ttgtcaccgt gctgtgggcc gtgctcgcct accgtgcacg ctcggtgttt gccgtgctct
     1981 ttcagaactc ttactaaacg gagtccctta gaacttcgct ttcttcggag gtttcgactt
     2041 gccagaacgg ttgagggcga ccgcttggcg aacgagtgcc ttgaaggcgg actcgtcaac
     2101 ttcttctcct tcgtggatgt cgatcgcgcg gcgtacgttt ccgtcgagac tcgagttgaa
     2161 gagtcgggcc gaatccgtca gagacgcgcc cttggcgaag gtcagcttca cgaccttctt
     2221 gtaggattcg cccgtgcaga ggattccgtc gtgcgaccaa actggaatgc ccaacttcca
     2281 ctcctcgacg acgcccgggt ctgcttgctg gatgagctgg cgcattcggc tgagggtttc
     2341 cccgcgccag tctccgagtt cggcgattct tttcgagatg agctccgatg cgggctggac
     2401 ttggctcgcg tccgactttt tcatcctctc ctccagaagt cccagatata tcacctgccc
     2461 taggttctta aaatgaaaat gcggagcggg ccacccgccg acctctttaa tttttgtgtt
     2521 ctacgtaact acaacacaaa ctcacaagta tgaagttatt taaactaaag caattacaaa
     2581 attagcatgg gtaattgttt acagttatac ataactataa cactatatca cacggcgaaa
     2641 gcggtcgaaa tcagggagcc agtgcaacgc cgacctaggc gagttgtcaa agagccgcga
     2701 gcagcggcgg cttgggccgc aaggtgaggg acacgaatcc ctccccgtca tgacctataa
     2761 cgcggcggcc gaagtagaag acatagaaat cggcttcgag acgaaactaa agtaaccagc
     2821 ggttcccgga atgggaagag ggcatcgcgg aaaagacccc gggcaacgga aggcgggata
     2881 gggcacacgc ctgtagattt ctagaggcgt aggggacgcg cgcgggccag tcgcccgtgg
     2941 ccctaagcac ccttcttctt gatccaaagc ggaagaagtg gatcgaggaa gaaatagttg
     3001 ccgtggtagc gatcaaggat gtccctggaa tcgagggact gcagagcttt ctgcaccgtc
     3061 gaggccgggc ccagtcgaaa ctcttcccgc actgcctgag agtagatttc cgaagggccg
     3121 cgcgcggcca ttgcctgtag agctgcgcgc tggcgggctg caagttgttc ccacaggagt
     3181 tcgtagtacg tggattgtcc gctgaccagg gcaccaatga ccgaatcgac gtcggcgacc
     3241 gtgagtcgtg gctttttctg caactcagca gtgtcccaca gttcgtgcgc aatgcgctgc
     3301 acgtcgtagg ggatgaggtc ggcagcggtg agaagagcgt cgatcgcctt ctcgtcgagg
     3361 gtgcgaccgc gcttgtggaa ctgggcggta atgaactcct tccagtcggc cgcggcgatc
     3421 ttgtcgagga acatctgcgg gcccgcctta tgaaaagggc ggctggcggt gagcatttcc
     3481 tgcatcagcg agggctggga accggcaaag acgtagccga cgccgcgctg ctcctgcacc
     3541 tgattgcgga tgacgttttc gacggagcct ccgttaaact gcgtaatctg ctggaactca
     3601 tccaggcaga tcgccatacg aaagccgccg ttgcgagtga gttctccggg gagggcgaag
     3661 atttccggcg cgatgggtgc gggatcaaag cccgcccctt tgcccagcga caggctgact
     3721 tcgccggtcg taatgttgta gttgacgtcg ggtttcacct ggcggccaaa gcgggtgatc
     3781 cagtccttga cgcggtccca gggaccggcg gcgcgcagga ccttttcggc aaacttttcc
     3841 aggaactggg cgtagctgga gtagctgctc acggtgaggc tgacggtacg gatgtgccgc
     3901 ttcttcagct tgagcagtgc catggctacc agcgagcttt tgccaaagcg gcgcggggac
     3961 aaaaggaaca ctttctggcc gtcggcgagg tcgcggacga gttgccgaag ttcggcggtg
     4021 cgattgacga aattctcgtc gtcgatgatt tcgccgaaga tgaagggatt ttcgtggttg
     4081 cccatattac gctatgtagc gtaacgctac atggcgtaat gtgcaagcga tttgttacgc
     4141 tagttggcgt aacgctgaat ggggtaatgg agtatcggga gttgggaacg aaaatcccca
     4201 ccccgcctat ctgtccccag cagctttggc cgctttggca gccgcggcct gcgcccgaag
     4261 cttttccgcc caggcggtaa atgccgccag cgtggcttcc ctcagggtca agccctggcc
     4321 cgcggccatc atcttgatgt tgcgcttttc gttgtcggaa aggcgaacct gaagcagatt
     4381 tcgttcggtc tgaggtgctt tttctttcat tttgttttca tgcctattta gaatcagctg
     4441 gttagccaga aattcttcgc aagcaattga tttcagcgga cttatacgta tccatatgaa
     4501 ccataatggt ttaggtgcga tttcgagcgt tttttggggc ccaagttgtc atataacaca
     4561 tacaagtcat acacgaaatc gtcatacatt gcatacaagt atgacaagtt tttgtcatac
     4621 attccataca aatatggcaa agcctcaacc gcagctgtgg gggcacgccg ggatttcaaa
     4681 gaacggcttc aggtccgcgg actgggcgcg aaccgagtcg agacacacca atccctctcc
     4741 tcgcagcaat ggttcactca agggaggcgg atgcgaaagg gcaccggtca cggtagggtg
     4801 tgataaccgg cagttggcag cggtcgggtg tcggttgtga agagaacccc acatatccca
     4861 aaatcaggga tatgtggacc accggcgaac ccgggggaac ccgccacgcg gcgcgtcctt
     4921 gaacggggca gcctcgaggt ttcatacgag aagtggataa cgatggctag cttggttgcg
     4981 gctagccatc gcggatcggc cttcgctttt agttccaata aggcttcgcg ccgtaatacg
     5041 tgtaaacttt ggcgccccac acacggtcag ccatgtcggg ccaatgctcc ttgtcgaaac
     5101 cgggagcggc cttaagcaat cccttgtcga cgttcagaat gaaccgatgt tcaaccgtgt
     5161 ccaccgaaag cgcgctccat ggaatggcaa acagcttatc gcccataccc aggaaaccgc
     5221 cgaacgaaag aaccgcgtaa gcgatgcggc ccgactccag gtcgatcatc aaatgttcga
     5281 tcttcccgag atcttccttt tggaaattta cgactttgtc gccggttaga gtggttgcgg
     5341 acagtactgc cggtgttctt tccattgttg ctgtcatgat gtctcctttg tctcgccgta
     5401 ttcaatttga tgaattccgg gtaatacctg cttacatacc gagatatagg ccttcgagag
     5461 tcgcgagtcc ggtcaagatt gaccgtccgg cctgcggcgt gttccagaat tcctgttgtg
     5521 gttgttttct tagcgtgggc ggcatttcca accgtaggtt tttcagaggg gtggagacgt
     5581 gagaggccgc ccgccacgag cagatcccag gttagcccaa aggaagggcg aacccttcga
     5641 caagctcagg gcaggctctg gaacccggct gtatgattga cagtcgttgt cagatatgac
     5701 aacatcgaga ggggttactt acgaggggaa tgccgcgatg aagagcgaga agctggaagt
     5761 ggtacggggg agcgggaatg cgttccggga ccttgggcac aagagcgcgg acgcggagca
     5821 gttcaaagcc atcttggccg ccgagatcat caaggcgctc gaccgcgagc ggcttaccgt
     5881 gcgcgcggcg catgctcgaa cgggtatcgc agccgccgat ttctcgcgta ttcgcagcgc
     5941 cgacttgggg cgcttcacgg tggaccgcct gatttctgtg ttgaaccggc tgggctcgcg
     6001 cgtggaggtg aaggttcgcg tgcatcgcgc tacgggcagc gtgcaagagg cgcgcgtggg
     6061 agcgtaggac ttgggaaaag acccaccccg ccaacggcag gcgggattgg gcacccgcgt
     6121 atgataatgt ccctttgaag tagcgcacac ttacaatcgg ccagcaatga attggaagaa
     6181 tccccggtca gtagaagcaa cgatcacccg actgacgcga gaaatcgcga agatcgacga
     6241 agtcttctac atggtcaatg aaaccgatga tcgtgtccta tatgccggca tgctcgaacg
     6301 caagcgcgat gacgtcgttc gggccgcagt tttgcaaatg catactgcca tcgaagacct
     6361 tctgaactcg cagattattt gtcgcattct gaacgtgaaa ccgcaggatc gcgcccgaaa
     6421 gatgcgtagt aaattagcgc aggcccttcg cagcatgctc gttggtccga gaagcctcgg
     6481 cttcgagatg aagctcaact ttgccgtcgt gctcggaatt gttaggacag gaacgaaaga
     6541 cagactcacc gaattgaata ccctgcgaaa caagtgcagt cataattggc tcctgaaggc
     6601 gccggtgaga cgcggaaaac gaccgaagca aaagaagtcc cctttgctcc tgtacagagg
     6661 acgagatctc tacaatgtcg atgtcctcgc agaatttcta gccgagtatg gccctattta
     6721 ttacaagctg ttcggcagat atttggattt gtagtgccga ggacccaccc cgccaacaga
     6781 aggcgggatg gggcaccctc tcaaagattt ttcaagagta tacgagcgcg gggcgggcca
     6841 cccgcctcga aatgaatcat tcaataccgc tctggccaag gaggctcaaa tggcggcttc
     6901 cagacttctg atcgtagaat caattaacga cgatctcaca aagcgacgaa ttgcgagtaa
     6961 acaagccaaa tttgcagcgg atgacctttc ggttgagtat tgctatgcct tggaatcgac
     7021 acgtcgtatc gacaagcaaa gtcttctcga ttatattgac cggaaaatta attcgttccg
     7081 tcctgacttg atgatcgtcc atgctggatt cgtattctca caacatccgg aaactttctt
     7141 atcagtgctg acagagctga gctctaagca tccgttgtta cgaatcggat cccagatgtc
     7201 tgagattgaa cccggctgga aatcccgttt tgcgctggtc tttgaagaca ctaaggagat
     7261 cagagatttg gcgatgcgtc tcgtctaggt attacggcga ctgtcccgcc gctgttcaac
     7321 ttcgggacgc aacctgcgac ttcttggata ttccgagccg attcgacagc tctttgatca
     7381 gagcgtcgcg atccttcttc gttagaatgg atcgtgcttg ggatctggaa agggcctcag
     7441 gaacttcgct gacatccaca aaagccaagc acggggtgac atgttttccc aatgcccagc
     7501 ccgcaccgac ctcgatcatg atccaaggcc tgtcctttga gttaggtgtg ataacaacca
     7561 gcatttcgct acacgcgacg atcgcctctc ggatgcttgg ctcccataga tccccaccct
     7621 tcacatctcg tgaggccatg aaggcaatga tccctcgcct agttagggct tctcggagtt
     7681 cttcggcaat cttttggtct ttagccgcat agctgatgaa gaccctactc gatttaattg
     7741 aggcagcgat gaggcttgcg tgacagaaac gtttgtagtc atcaaacgta agcatcttgc
     7801 gtaaccgctt ttcgtggaag atctccatga actgcactct tactaaatcg tccgagttga
     7861 ttacggacac tattgcgcgt tcgagttctt cttcggacaa gtatcttggg gggtatcgac
     7921 gatagacgat atcttcgatg cgactcggta cacatcgctt tcgggatgaa tccgaccaga
     7981 gaagagggtc gacagagcgt cgggatctgc tggagtcctg ctcgtcagaa ctatctgcgt
     8041 gctcaattta tctcggtgac ggagctcaga aagcgtaagc gctgcacacg gaggaagaga
     8101 cagatgcagc aaaatcgccg agtaatcgct cctgagaata tccagcagcg gtcccatggc
     8161 tgaaacctgt ttccaaggaa agaaaacaag ctcccatgtg ggttcggcac ttcgtatctc
     8221 atgttcgatt atggatcgtt cattctcgtc gttccaaagg catgcgagtt tcatgatgcg
     8281 caccggcctc gccgctaagt aatgagctta gatgatcaaa gccatgctac cgataccatt
     8341 agtcatgctg caatgaaaaa ctactcttcg gcacctgaag attcggggtg ggcttgcgcc
     8401 caccccgctc aaggccgggg ctgaagcccc ggggttgatc gttaacgtcc agcggcacgg
     8461 ctaaaggcgt gccctttcaa agcaactaca cgagcccctg gtccagcatc gcattcgcta
     8521 ctttcaggaa gccgccgatg ttggcgccgt tgacgaggtt gccgggcgtg ccgtacatct
     8581 cggccgtctc gtagcagttt ttgtggatgg cgatcatgat cttgtgcaaa cgatcgtcca
     8641 cttcctcgcg ggtccaggtc aggcgcatgc tgttctgcga catttccaga cctgaggttg
     8701 cgacaccacc ggcgttggcc gccttggccg ggccgtagag cagtccggct tcgaggaaca
     8761 gtttggtggc ttcgagcgtg ctgggcatgt tcgcgccctc ggcaaccagc ttgcaaccgt
     8821 tattgatgag gttggtggca tccttcacgt tgatttcgtt ctgcgtagcg ctcgggaagg
     8881 cgcagtccgc cttgatgttc cacagcgggt tgtagccgag tttcacgtcg gacggcatga
     8941 agatggcctt cttgtactgg tccgtatagt cgcggatgcg gccacggcgg acgttcttca
     9001 gatccatgac aaatgccagc ttctcgcggt cgatgccatc gggatcgtac accacgccgt
     9061 cggagtcgga gaccgtgact ggcttggcgc cgaaatcaag cagcttctcg acggtgtact
     9121 gcgagacgtt gccgctgccg gagaccaggc agagcttgcc ttcgagggtg tccttgcggg
     9181 acttcagcat ttcgtcggcg aaatacacgc agccgtagcc ggtggcttcg ggccgaatga
     9241 gcgagccgcc ccaggcaagg cccttgccgg tgaggacgcc ggcaaattcg ttgcgcaggc
     9301 gcttgtactg gccgaacagg aagccgatct cgcgtccgcc cacgccgatg tcgccggcgg
     9361 gcacgtcggt gtcgggaccg atgtggcggg agagttcggt catgaagctc tggcagaagc
     9421 gcatgacttc gttgtcactc ttgcccttgg gatcaaaatc ggaaccgccc ttgccgccgc
     9481 ccatggggag cgtggtgagc gagttcttga agacctgctc gaaggcgagg aacttcagga
     9541 tgccgaggtt gaccgaggga tggaagcgca ggcctccctt gtaggggccg atggcgctgt
     9601 tcatttcgat gcggaatccg cgattgacct ggatgttgcc atggtcgtca aaccagggaa
     9661 cgcggaacat gaggacgcgc tcgggttcgg tgatgcgctc gagaatccgc tccgagatgt
     9721 actcggggtg tctctccaac acaagacgca acgactccat gacttcctgt acggcctgat
     9781 ggaattcggg ctcggccggg ttcttggctt tgaccagccc cataacttcg ttgatgtagt
     9841 cgcccggggt cttaatcccg ggttgcatta ttcctactgt cataagaggt cctcctacga
     9901 accttacttc tgattgcact gcttaaccaa acacactcgg gggccaagac tctcggtaca
     9961 ggactgcctt aaccgatcat caggcgcaca gaatattgga gtcccgatcg ggcggctctg
    10021 ccgtgaccgc tatcacccga gaacgtgaca gactggccaa agagtaacgt ttgtaacagc
    10081 cgcatcggaa ggaaagtaca ccaattcagg gcaggaaaca atggtggatt ggggtgggtg
    10141 tcggaagagg catcttcttc gggaaacaat cgttgatgag gccatgggcc agtggcagcg
    10201 gcgtggcaaa ggtgccgaag agcaaatccc tcgggggctt aacttcaccc cacgaagcgc
    10261 aagtacggcg cttcgcgggg accccggctc gccccctcgt gatgacaaga atgaagggct
    10321 tgaacggcac ggctgaagcc gtgcccgttc aaaacaggtc gaaggtcgac ttttcagcag
    10381 ccgctgaagc ggtgctccac cctttcttcc atatacccca ttctgacccg cggaaatcgt
    10441 gataggccct atcaattgtt cttattagca cagggtatgc ctcacctgct aggttagagg
    10501 actcatggcc agcgcaccgc tccaggctct ccatcgcgga ctctttgagg gctacgatac
    10561 gggtgcgttt ttcgacgaga tgttcgtggc gccgggcgtg ccccggccgc attacgccaa
    10621 gctgttccag atgctgggat cgatacccca gacgcagttc gaagagcggc gcaacctggc
    10681 cgatatcgct tttctgctgc agggcatcac gttcaccgtg tacaacgatg gcggcaccgg
    10741 caccgagcgg ctgttcccgt tcgacctgat cccgcgcata ctgccgcatt cggaatggat
    10801 ccacatcgaa cgcggcctgt cgcagcgagt ggtggcgctc aacctttttc tgcaggacat
    10861 ctatggacaa cagcgcatcc tgaaagagcg caagattccg cgctcgctga tttactcgtg
    10921 ccaccatttt caccgcgaga tgattggcgt ggaagtggcg cacggaattt acacgcacat
    10981 ctgcggaatc gatctggtgc gggactcgaa gaccggcgac ttcctggtgc tggaagacaa
    11041 cgtgcggact cccagcggcg tttcctatgt gctggaaaac cggcaggtga tgacgcgcac
    11101 tttccccgac gcgtttcagt cctacgaagt gttgccggtg catgactacc cgacggagtt
    11161 gtgccgcatc ctgcgaaccc tgtctccgcg cggcgaagac aacgcgcaga tcgtgcttct
    11221 gaccccgggt atttacaaca gcgcctattt cgaacacagc ttcctggcgc agcagatggg
    11281 catcgagctg gtggaaggcc gcgacctcat cgtcgatgcc ggcgtggtgt acatgaagac
    11341 catccacgga ttgcagcgag tggatgtgat ctatcgccgg gtggacgacg agtatctcga
    11401 tccgctggtc ttccgtcccg aatcgctcat gggagtgccc ggcttgatgg gcgcataccg
    11461 cgcgggaaac gttgcgctgg ccaacgccgt gggcaacgga gtggccgacg acaaggcggt
    11521 gtgcgcatac gtgcctgact tcatccgctt ctatctgggc gaggaaccga tcctggggac
    11581 ggtggaaacc tatctgtgct cgcggccaga tgacctgcgc tacacgctgg cgcacctgcc
    11641 ggaacttgtg gtgaaggcag tcggtgagtc ggggggatat ggcatgttga tcggtcccgc
    11701 gtcggacgag gagaccatcg aggacttccg caagaaggtc gaggccgacc cgcggaatta
    11761 cattgcgcag cccgtcatcg gtttgtcgcg cgtgccttcg tacgatgtgg agtccaagcg
    11821 catggcagga cggcacgttg acctgcgtcc ctactgcttg tatgacgggc agggtgtgac
    11881 gattatccca ggcggtctga cgcgcgtggc gctgaagccg ggttcgctgg tagtgaattc
    11941 ttcccagggc ggtggcagca aggatacctg ggtcctgagg gaagactcct gagcaccgta
    12001 atgttgtcgc gcatcgcgga ttcgatgttc tggctggcgc gttatatgga gcgcgccgag
    12061 gacaccgcgc gcattctcga cgtcaactac catatgctgc tggagc
//
LOCUS       DASRKK010000384         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1104806, whole genome shotgun sequence.
ACCESSION   DASRKK010000384 DASRKK010000000
VERSION     DASRKK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104806"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFC15_08575"
     CDS             complement(<1..463)
                     /locus_tag="VFC15_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10250.1"
                     /translation="MLPQTVTTVWRTKLGTTLLIGFNNSTGLSAYFRYLGISQSPPKR
                     RITPSSSPVWPRGTRALASQELAATREMMRRNDRLTPLERVKQEQEEERDRILPVPEL
                     QAGTNGAITLTMVNQSPKVLFETVGKYAGMNVLFDPEYQPGKNFSLTLDSVT"
     gene            540..2000
                     /locus_tag="VFC15_08580"
     CDS             540..2000
                     /locus_tag="VFC15_08580"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10251.1"
                     /translation="MSKPKKQPPKSAEEKAQASKGKASKSKRGRRRKGMCLEDRPLLE
                     PNAGGIDIGAREIFVAVPPDRDENPVRVFSTFTEDLEAMARWLVSCGITTVAMESTGV
                     YWIPPYDVLEQHGIKACLVDARGMKNVPGRRTDWHECQWIQFLHSVGLLRAAFRPDGE
                     VCAVRSLMRHRGDLVQMASQHIQHMQKALTQMNVQIHHVISDITGLTGLAIVDAIVGG
                     QRDPLELAKLRDPHIKASEETIRKSLVGNWRGEHLFTLKQSRQMYQHYQEQLAACDEE
                     IEKLLVAFQPWVDPEEKPLPPDRKKKQRGRKKKNVNPKTGFDLRTESYKLFGVDLTQV
                     PGLMGMILTLFGEVGRDMSRWPTSGNFVSWLALCPDNDITGGRVVWKGMRTVHNRAGD
                     LFRLAAYSLHHDQSQLGDYFRRMKSKMDAPEATTATAHKIAIIFYTMVKKQVEYDATI
                     WQQRDAEREKRFEAKLRRQAQQRGYKLVPIEEKPAA"
BASE COUNT          496 a          568 c          732 g          406 t
ORIGIN      
        1 gggttacgct gtcgagagtc agcgagaaat tcttgccggg ttgatattcg ggatcgaaaa
       61 ggacgttcat gccggcatat ttgccgacgg tttcaaagag cacctttggc gattggttga
      121 ccatggtcag ggtgattgcg ccgttcgtgc ccgcctggag ttcgggcaca ggcaggatcc
      181 ggtcgcgctc ctcctcctgc tcttgcttca cgcgctccag cggcgtgaga cggtcgttgc
      241 gccgcatcat ctcccgggtg gcggcgagtt cctgtgaagc tagtgcccgc gtgccacggg
      301 gccatactgg cgaggaactt ggcgtgatgc ggcgcttcgg gggagactgg gagataccca
      361 ggtagcggaa gtaggccgat aagccagtgg agttgttaaa cccgatgaga agcgtggtcc
      421 caagtttcgt gcgccataca gtggttacag tctgaggcag catcccaggc tgattccgat
      481 taaaagagtg cttccctttg cgcgacttgg ccgttgcctg agcagaaagg atcaggtgta
      541 tgtccaaacc gaagaagcag ccgccaaagt ccgctgagga aaaggcccag gcgagcaaag
      601 gcaaagcgag caaaagcaaa cggggacgaa gacgaaaagg gatgtgcctg gaggaccgcc
      661 cgcttttgga gccgaatgca ggcgggatcg atatcggtgc gagggagatc tttgtagccg
      721 tgccgccgga ccgggacgag aatccggtgc gggtgttttc tacgttcacc gaagatttgg
      781 aggcgatggc ccggtggctg gtgagttgtg gcatcacgac ggtagccatg gagagcacgg
      841 gggtgtactg gattccgccc tacgatgtgc tggagcaaca cgggatcaag gcgtgcctgg
      901 tggacgcccg tggcatgaaa aacgtaccgg ggcggcgcac ggactggcac gaatgccagt
      961 ggatccaatt tctgcattcg gtaggactgc tgcgcgcggc atttcgaccg gacggcgagg
     1021 tatgcgcggt gcggtcattg atgcggcacc gcggcgatct ggtgcagatg gccagccaac
     1081 atatccagca catgcagaaa gcgctgacgc agatgaatgt gcagatccat cacgtgatca
     1141 gcgacatcac cgggttgacc ggtctggcca tcgtggatgc catcgtcggc gggcaacgcg
     1201 acccactgga gttggcgaaa ctgcgggacc cgcatatcaa agccagcgaa gagacgattc
     1261 gcaaatcgct ggtgggcaat tggcgggggg agcatctttt cacgctgaag cagtcgcggc
     1321 agatgtacca gcattaccag gagcagctcg ccgcttgcga tgaagagatc gagaagttgt
     1381 tggtagcgtt ccagccctgg gtggaccccg aggagaagcc gctgccaccg gaccggaaga
     1441 aaaaacagcg tgggcgcaag aagaaaaacg taaatccgaa gacgggtttc gatcttcgaa
     1501 cggagtccta caagctattc ggtgtggacc tgacgcaggt tcccgggctg atggggatga
     1561 ttttgacatt attcggcgaa gtggggcggg acatgtcgag atggccgacc tcggggaact
     1621 tcgtgtcctg gctggcattg tgcccggata acgacatcac tggagggcgg gtggtgtgga
     1681 agggtatgcg cacggtgcac aaccgggcgg gggatctatt ccgactggcg gcatattctc
     1741 tgcatcacga ccaatcgcaa ctgggggact atttccgccg gatgaagagc aaaatggacg
     1801 ccccggaggc caccacagcg accgcccaca agatcgcgat catcttctac acgatggtga
     1861 agaagcaagt cgaatacgac gccacgattt ggcaacagcg cgacgccgag cgcgagaaac
     1921 gattcgaagc gaagctcaga cggcaggctc agcaacgagg ctacaaactg gtgcccatcg
     1981 aagaaaaacc tgctgcgtaa ctggctgaat tgaaaggctc ggggaaggag ttccttaaaa
     2041 ggcttgcgta gatcgcctgg cgcctcacgc gcaaatgccg agagcgctat ttgggccggc
     2101 tccagacggt gtgaagtact atttgtgagc gcgggcgcca caccgccgcg ccggcgtgcg
     2161 aaagccgaac gactctgccc attcgcttcg attgattatc aa
//
LOCUS       DASRKK010000385         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_29720, whole genome shotgun sequence.
ACCESSION   DASRKK010000385 DASRKK010000000
VERSION     DASRKK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29720"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFC15_08585"
     CDS             <1..186
                     /locus_tag="VFC15_08585"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HZM10252.1"
                     /translation="YYQLIERFGQATGVPAILNTSFNLKGEPIVNTPANAYSTFTKSE
                     MDTLVLGNFVVEKSDQP"
     gene            complement(373..573)
                     /locus_tag="VFC15_08590"
     CDS             complement(373..573)
                     /locus_tag="VFC15_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZM10253.1"
                     /translation="MNSTTAVKDPVCGMDVEPATAAGRTEHKGQTYYFCGSKCKEKFD
                     FNPEQYLGKSAGTPKSSHGCCG"
     gene            1499..1963
                     /locus_tag="VFC15_08595"
     CDS             1499..1963
                     /locus_tag="VFC15_08595"
                     /inference="COORDINATES: protein motif:HMM:NF042673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6629 family protein"
                     /protein_id="HZM10254.1"
                     /translation="MANVRAVRHGVMEAVRWRKKAIFAFEAAGVAVGLYLLYFIVTRP
                     VVAEVVGKHIVYVSPHFYLIPVMVSYLAATCVSCFFSSHGLVKLFGVLALLSFIAAYL
                     FHAMAFFSIWCFFAAILSLLIYLRLRFRDLGGFPKEAALPPAIARGAVTEPE"
     gene            2178..2258
                     /locus_tag="VFC15_08600"
     tRNA            2178..2258
                     /locus_tag="VFC15_08600"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT          505 a          709 c          693 g          624 t
ORIGIN      
        1 tactaccagt tgatcgagcg cttcggccaa gcgactggcg tgccggcgat actcaacacc
       61 tcgttcaatc tcaaaggcga gcccatcgta aacactcctg ccaacgctta cagtaccttc
      121 accaagagcg agatggacac cttggttctg ggcaatttcg tcgtcgagaa gtccgatcag
      181 ccctaggtga tcacggagtt ttataccagg gaccagagcg gcattcccac cgcatgggtc
      241 aagcgtatgc gggaaagcat ggcgcgcttg acgccccgct tctctgcgag ccgcgcagtg
      301 cgtgaatgag ccacacatag cggaagctag ggtcaacggt gggaatggct tttacctgcg
      361 gcgttgttgg atttaaccgc agcagccatg gctgctcttc ggcgttcccg cagacttgcc
      421 caagtattgc tccggattaa aatcgaactt ctctttgcat ttggagccgc agaagtaata
      481 cgtctgccct ttgtgctcag ttcgtccagc ggcggtagcc ggttctacgt ccatgccgca
      541 cacaggatct ttcacagctg ttgtgctatt cattatgatt tctccttcat tcgtgaattt
      601 tggctcgatg gtagttcaaa tgctgatcgg ccggtttcag caactcatca tggactgtcc
      661 ttatctcgtg tcgttcttgg gcaggccata acttccagcc gtggccactg gcgcccaaac
      721 cctgacagga actttctcat gtcggggatg atgaggtctg tgcattcaag ctcacttcaa
      781 gcatgcggcc gatcaatttc atgtgaatgg aaagagggtg aatcaggaag agatctacag
      841 attgccagcg gcaaagttgg aagccaactc gacgaaagat tcgctgcatt atccacctga
      901 agtgactcgc cggtaaaggc tgattcgcgc gcatgcaggt ctctcaacac gagcagtcgg
      961 gcgtcggatg ggatgaggtc tccagcgttt gaccggatga cgtcccccga aaccagctct
     1021 gcgatcggaa gttcctgttc gtgcccgtca tgaataacag ttgccacttg tggttgccaa
     1081 tgcgaggcag gcgaaactga tcagcgacag cactcgggaa agatgatcgt ttggatgcac
     1141 taactctaca cctacgaaga ctggcgccag agttccgccg tcggccgcat ggcccagcga
     1201 tagtctcact cgcagccccg ttgaaagtag gatggacggt cgtatgtaca aggaaaggat
     1261 cgtgtccaat gtgtttttca gcctcggcca gtttcatcac gggcagttca ctttccgctg
     1321 tcggcgtggc gaccctcaga agaacggaga ggacggctgg gctacctttt gccatgattc
     1381 cgttgctgtt cgggatccag cagctgaccg aaggcgtcat ctggctcaca ttccgccata
     1441 acgcaccgct cctcaagcag acgatgactt atctgtactc gggattctcg cacgttctat
     1501 ggccaatgta cgtgccgttc gccatggcgt catggaagcc gtacgctggc gcaagaaggc
     1561 gatcttcgcg ttcgaggcgg cgggggtcgc ggtcggtctc tacctgctct atttcatcgt
     1621 gactcgcccg gtggtcgctg aagtcgtcgg caagcacatc gtctacgtgt cgccacattt
     1681 ctacctgatt cccgtgatgg tgtcctacct tgcggcgacg tgtgtcagtt gcttcttctc
     1741 gagtcacggg ctcgtgaagc tgtttggcgt gttggcattg ttgtccttca tcgcggccta
     1801 cctctttcat gccatggctt ttttctcgat ttggtgtttc ttcgcagcga tcttgagtct
     1861 tctcatttat cttcgcctca gatttagaga tctcgggggc ttcccgaagg aagccgcgtt
     1921 gccgccggcc atcgcgcgcg gcgccgtaac ggaaccggaa taggatgacg ttgagcgcag
     1981 gcgtcatcgc gtggtcgata tcccgaacct ggaggtccgg tgtgcagccc aaccaatttc
     2041 ggttgccaca ttcgttcccg cgttgagtcg gcgccggtac gcggcgcacg gattcatagt
     2101 gatagcattg tcgtgcaaat tgtcgagcaa aacccctcag gtatagcggt tatgaccccc
     2161 ttgaaccgct agaaaagcct ttagaatcaa tcagcagctt gcgtacttcc cggctgttaa
     2221 ccggagggtt gtaggtttga gtcctacctg aggagccatt ctttctacac tgagcggggc
     2281 gacgattgat ccttgccctc ttggacaaga cgaagctatg caagtgcttg gcctggtgct
     2341 cgacgaggaa gtttttttca gtccttcaag cgttcgcccc caacatggtt ccgcaaacac
     2401 gcgatcgtct cggaatgcgg cttgcccgcc ccgcttaagc ctttgggatt aggctggcgg
     2461 cctcgccgct gtttgcaaaa ctccaacttc accagcagcg tcctctcgac gttctttcac
     2521 agtcctgcag a
//
LOCUS       DASRKK010000386         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_811449, whole genome shotgun sequence.
ACCESSION   DASRKK010000386 DASRKK010000000
VERSION     DASRKK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811449"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFC15_08605"
     CDS             <1..240
                     /locus_tag="VFC15_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM10255.1"
                     /translation="AYIWLLKHVSTQKVATYAYVNPIVAVFLGWLVLHERFDGYIFAG
                     SVVIIAAVALVTTAKVHSREPGEQSGGLPELESEV"
     gene            314..565
                     /locus_tag="VFC15_08610"
     CDS             314..565
                     /locus_tag="VFC15_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10256.1"
                     /translation="MEVHFTPETEKKLKDLSTQSGRRTDDLVEDAMAGYLDEVLQARE
                     MLNSRYDDLKSGRVKPISRDQVIAHFREKSAARRSPPDS"
     gene            complement(879..1052)
                     /locus_tag="VFC15_08615"
     CDS             complement(879..1052)
                     /locus_tag="VFC15_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10257.1"
                     /translation="MQKQRQQIPRIKPLDQLDAYSHLIDVLRKIGEQLEAINTQLEAL
                     NLGIKTLSNSRQT"
     gene            complement(1164..1544)
                     /locus_tag="VFC15_08620"
     CDS             complement(1164..1544)
                     /locus_tag="VFC15_08620"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HZM10258.1"
                     /translation="MVNSRMKQVLFTLLLCLPATAQEATLPDLGKSGNDYLHSCEALT
                     HNRDFSVQDVQCGTFTKGVVEGLTAYQIQSRGKLFNVPPKITVPQIEKIVVKYMNDHP
                     KNLDLATSQIILWALIDAYPPPKK"
     gene            complement(1650..2312)
                     /locus_tag="VFC15_08625"
     CDS             complement(1650..2312)
                     /locus_tag="VFC15_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HZM10259.1"
                     /translation="MMAMFTAYFDASGNAIDQPFIIVSGYIANLFQWREFEKQWAAVH
                     KQYSVNLPFHMAEFMAALNNPQYKTQKNARADYVDIAKDPDIAKGFLGQLSILQAVMV
                     NCGVSCVVTMDIYNDVSPLLDLRKVVPPYALGARMCIERIHRWEEQFASIEPVECIFE
                     EGDFEQGKFTDLMVDEGRDPPIYKKKEILLDSRPPITTHGSRHLFSRNMHTAAPRNLG
                     QF"
     gene            complement(2553..>4044)
                     /gene="cysS"
                     /locus_tag="VFC15_08630"
     CDS             complement(2553..>4044)
                     /gene="cysS"
                     /locus_tag="VFC15_08630"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZM10260.1"
                     /translation="SMPLQLFNTLSGKVEEFHPLEDNLVRMYACGPTVYDYGHIGNFR
                     TFVVVDTLRRFLKQSGYKLKHVMNITDVDDKIIRNAAASGVSVQDYTQKYKQAFLEDA
                     DSLNIQHPELLVNATDHIQEMAHFIKGLEEKGFAYRTDDGSYYFRIAKFPEYGKLSKR
                     DFAGMNDGARVDVDEYDKDNARDFALWKAPKPGEAKWETEIGPGRPGWHIECSQMSMK
                     YLGESFDIHAGGEDLIFPHHENEIAQSEALTGKPFVHVWVHARFLLVEGEKMAKSAGN
                     FFTVRDLILMGHKPSSIRFLLMSVPYRKQLNFTFDGLTQAANSVERLRNFKLRLETSP
                     FPDDGNVIIAEIAERTSAEMRAALDDDLNTARALGAVFDMVRDVNAAADAGEVRKGDI
                     PQLLRVLEEFEEIFAVLTDDDAAKVRATVEWAKTEGLEDKISPQTAELAKAAALSDAD
                     VEQLVAEHSQARKTRDFARSDAIRAQLAENGVILENTKDGVRWRRK"
BASE COUNT          856 a         1097 c         1117 g          974 t
ORIGIN      
        1 gcgtacatct ggttgctgaa gcatgtgtcc acgcagaagg tggcgacgta cgcctacgtg
       61 aatcccattg tcgcggtttt cctgggatgg ctggtgctgc atgagcgctt cgacggatac
      121 atatttgccg gaagcgtagt catcatcgcc gccgtggcat tggtcaccac ggcaaaagtt
      181 cacagccgtg aaccgggcga gcaatcgggc ggattgcctg aactcgaatc ggaagtgtag
      241 gggtaggcgc ctggtctggc cctacgtccc tcgaccgttg tcaggcagcg gtttttctcg
      301 tactctgaag gcgatggaag tacacttcac ccccgagacg gaaaagaaac tcaaggacct
      361 gtccacgcag agcggacgca ggactgacga cttggttgag gacgcaatgg cgggatacct
      421 tgacgaagtg ctgcaagcgc gggagatgct caacagccgc tatgacgacc tgaaaagtgg
      481 cagggtgaag cccatttccc gcgaccaggt gattgcccac ttccgcgaaa agagcgcggc
      541 ccgccgctcc ccgccggatt catgagcgga tacgatttcc acccggaagc gtggtaatca
      601 tcgccgctgt ggccttcgtc accactgcga aagttcaccc ccgcgaaccg ggcgagcaat
      661 cggtgggagt gcccgaactc gaatcggaag tgtagaccga ttccatagaa gtgtgatttt
      721 tgtggtacag atgtggtatg aaagccacag cgcaaggggg gactaagaag gtagtggagc
      781 tggccaagac ggtattcaat gtcataggcg gctctgacgt agatacagca atcgccgcct
      841 tgaggatagc ccgcaatctt ctggatcacc gcgaagggct acgtctgacg agaattactc
      901 agcgttttga tccccaggtt aagagcctct agctgtgtgt taatggcctc tagctgctcg
      961 ccgatcttgc gcaacacatc aatcaagtga gagtaggcgt caagttgatc gagcggcttg
     1021 attcgcggta tctgctgtcg ttgtttctgc atctgggaca catctccttt gcgctggcat
     1081 tgtgatctcg cgatttgggt tgcgattata cgcctcacca gatcgtatta ttgcagcctc
     1141 ggtgttacgg ggctggagcg aggctacttc ttcggcggcg ggtaagcgtc gatgagcgcc
     1201 cagagaatga tctgtgacgt tgctaggtcg aggtttttgg ggtggtcatt catgtacttc
     1261 actacgatct tctcaatctg cggcacggtt atctttggcg gaacattgaa cagcttgcca
     1321 cgggactgga tttggtaggc cgtcaagccc tcgacaacgc cctttgtgaa ggttccgcat
     1381 tgcacgtctt gcacggagaa atctctgttg tgggtcaatg cttcgcaaga atgtaaatag
     1441 tcgtttccag acttgcccaa gtccggcaaa gtcgcctctt gagctgtcgc cggaaggcag
     1501 aggagtagcg taaacaacac ctgtttcatg cgactattta ccatgcttta ccccggtcct
     1561 tgggtctata cccttcgcct gacaaagtct gattagcgtg gccgtggtcg gttcaaggtg
     1621 caattttgga attgcatgaa gcacaagttc taaaattggc ccaggtttcg tggcgctgct
     1681 gtatgcatat ttcgtgagaa aagatgcctg ctcccatgcg tagtgatcgg cggcctggag
     1741 tccagcaaaa tctctttttt tttgtaaatc gggggatctc tcccctcgtc taccatcaaa
     1801 tcggtgaact tgccttgctc aaaatcccct tcttcaaaaa tgcattccac tggctctatg
     1861 gaagcaaact gttcttccca acggtggata cgctcgatgc acatgcgggc tccaagggca
     1921 tagggaggga caacctttct taaatccaaa agcggactaa catcgttata aatatccatt
     1981 gtgaccacgc acgacactcc gcaattaacc atcacggcct gtaggataga cagttgcccg
     2041 aggaatccct ttgcaatgtc tgggtctttt gcgatgtcaa cataatcagc gcgggcgttt
     2101 ttttgtgtct tgtattgagg gttattgaga gcggccatga actctgccat atgaaacggc
     2161 agattcaccg aatactgctt atgtacggct gcccattgtt tctcaaattc tctccactgg
     2221 aagagattgg cgatgtaacc cgaaacgata ataaacggtt ggtcgattgc gtttcctgat
     2281 gcgtcgaaat aagccgtgaa catcgccatc aatctagctc ctggatgggc gcaatttaca
     2341 aatatggacg ctgccaatct gtcgagcgga cgaggattgt ccagcgagaa gcagtttacc
     2401 taatttctca gcgccgcact tggggcatcg cagatacggc cccatgatga agtcacgagg
     2461 gtctatcctt atcgtgcctg acatcgacaa aacctgttct tcttgccctc tctgcgcttc
     2521 tcaaagggaa aggccttaga gtttgcgtgg gcctacttcc ttctccaccg caccccgtct
     2581 ttggtgtttt ccaggatgac gccattctcc gccaactgtg ctcggattgc gtctgagcgc
     2641 gcgaaatcgc gggtcttacg cgcttgcgag tgctccgcga cgagttgctc aacatcggcg
     2701 tcagagaggg ccgcagcttt ggcaagctcg gccgtctgcg ggctgatctt gtcctccagg
     2761 ccttctgtct ttgcccactc gacggttgcg cgcaccttgg cagcatcgtc gtccgtcagc
     2821 actgcgaaga tctcttcaaa ttcttcgaga accctgagca gttgagggat gtcgcctttc
     2881 cgtacttctc cggcatcggc ggcagcattc acgtccctca ccatgtcaaa aacagcgccc
     2941 aacgcgcggg ccgtattcaa atcgtcgtcc aacgcggcgc gcatttctgc tgaggtcctc
     3001 tccgcgattt cggcaatgat cacattgccg tcatcgggga acggcgaagt ctccagccgc
     3061 agcttgaagt tgcgtagccg ctcaaccgaa ttggcggctt gagtcaggcc gtcgaaggtg
     3121 aagttgagct gtttgcggta aggaaccgac atcagcagaa atcggatgga cgatggctta
     3181 tgtcccatca gaatcagatc gcggacagta aaaaagttgc cggcgctctt ggccatcttc
     3241 tcgccttcca ccaacaggaa ccgcgcatgt acccaaacgt gcacgaatgg tttgccggtc
     3301 aacgcctccg actgcgcaat ctcgttttcg tgatgcggaa agatgagatc ctcgccgccg
     3361 gcatgaatgt cgaagctctc gccgaggtac ttcatcgaca tttgcgagca ctcgatgtgc
     3421 cagcccggac gaccgggacc gatctcggtc tcccatttcg cctcgcccgg tttcggcgcc
     3481 ttccacagcg cgaagtcgcg ggcgttgtcc ttgtcgtatt cgtccacgtc cacccgcgca
     3541 ccatcgttca tgcccgcgaa gtccctcttt gacagcttgc cgtactcggg aaactttgcg
     3601 attcggaagt agtaggagcc gtcgtcggtg cggtaggcaa agcccttctc ctcaagtccc
     3661 ttgataaagt gggccatctc ctgaatgtgg tcggtggcgt tcaccaggag ttcgggatgc
     3721 tggatgttca gcgagtcggc atcttccaga aacgcctgct tgtacttttg ggtgtagtcc
     3781 tgaacgctga cgcccgacgc ggcggcgttg cggataatct tgtcgtccac gtcggtgatg
     3841 ttcatgacgt gcttcagctt gtagccactc tgcttcagaa aacgccgcag cgtgtccacg
     3901 acgacaaagg tgcggaaatt gccaatgtgg ccgtagtcgt aaaccgtcgg cccgcaggcg
     3961 tacatgcgga ccaggttgtc ttccagtgga tggaactcct cgacttttcc ggacagagtg
     4021 ttaaagagct gaagcggcat ggac
//
LOCUS       DASRKK010000387        19437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_420576, whole genome shotgun sequence.
ACCESSION   DASRKK010000387 DASRKK010000000
VERSION     DASRKK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420576"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFC15_08635"
     CDS             <1..271
                     /locus_tag="VFC15_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10261.1"
                     /translation="ATGAGAFFGGGVGDLDAPLNSTVCVNAMFFLSLSTNVSVKVAEA
                     APNFFIDVASKLKLICVSVGTGNKASSMDAVTPFALNVRLLIVER"
     gene            complement(528..1930)
                     /locus_tag="VFC15_08640"
     CDS             complement(join(528..1568,1571..1930))
                     /locus_tag="VFC15_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558223.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM10262.1"
                     /translation="MPVGSIPDPEVAAKPARRRFTADYKRSIVEQSDACQDAGAIGAL
                     LRREGLYSSHLSTWRRQARQGELAALTPKKRGRKTTVSPLVEENRKLIAANARLTKRL
                     GNAELIIDVQKKLAALLGTDSRYPDRRGERMSAIQQLAVGVGQAAACRALGVPRASWY
                     RSRQPVALPAQRPTPARALPPTERQAVLDQLHAERFRDKAPAEVFTTLLDEGVFLCSL
                     STMYRILAENCEVRERRNQLRHPAYQKPELLATAPNQVWSWDITKLLGPVKWTYFYLY
                     VILDIFSRYVVGWMVADGESAQLAQRLIGNTCDKQLIVAGQLNLHADRGTAMTSKPVA
                     FLLADLGVTKTHSRPHVSDDNPYSESQFKTLKYRPEFPDRFGSIQDARAFCQHFFRWY
                     NGEHHHSGIAMLTPEMLHYGRAQEVIQQRQEVLNRAYIRNPERFVRACPTPQSPPTAA
                     WINPPTLTPTSENNAH"
     gene            2837..4303
                     /locus_tag="VFC15_08645"
     CDS             2837..4303
                     /locus_tag="VFC15_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM10263.1"
                     /translation="MNSVLAQLDPYIRSSLATTKVPGVSIAVIYNDQVVFLRGYGIRK
                     VGESAQVDPDTVFEIASFSKPITSTILASLVGEGKISWDDRIADIDPGFQLSSPDTTR
                     QITIRDFLSHRSGLATESGDLLEDLGYSRPIILYRMRYLPLPGQFRKTYAYSNFGYTT
                     GAIAAATKIGKPWETIAEEQLYSRLGMTSTSSRFSDYEDRANKAALHIFINGEPVNRF
                     VREADAEAPAGGVSSSARDLAEWVRLQLSGGKWNSKQIIDANALAETHKAQICRNPAD
                     PAKPNVCPGDQYYGLGWDVGTNAEGHTQVSHSGAFFLGAATAVYLVPEEHIGVLALSN
                     SLPVGLPESICLHFLDIVHYGKPQRDYLSLAAKLFSQMIAQTQDASPDYAAMTQPKTS
                     SPAQPLAAYAGKYTNEYFGTLEVSVENNQLVLRLPPRGSYYELAHWDGDTFTYYFASE
                     NTGAGRRGAIFSPEKNQVLIEMLSPEHNAVFARSQPEH"
     gene            complement(4360..7224)
                     /locus_tag="VFC15_08650"
     CDS             complement(4360..7224)
                     /locus_tag="VFC15_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZM10264.1"
                     /translation="MAVAVVTSAPVSSDEIKSSLASQIDGDRILVRPIDLIAFASDAS
                     FYRLIPKAVVLTKSLEEIKTLFEFSQHSGIPMTFRAAGSSLSGQSISDGLLVEVARHW
                     RGLNVEDGGKKIRVQPGVIGARANQLLAPYGAKIGPDPASIATCTLGGILSNNSSGMC
                     CGVEQNAYHTLHSLKFMLPSGTVIDAAQPDAEQQFHAREPELARGILDLKAHLESDSY
                     LRERVRRKYRTKNTTGYSLNAFLDFDSAVDIFQHVLIGAEGTLAFIAEAVLDTVPDLP
                     VKYTGLLLFPDLYAAADSIVPLRQAGAKALEVMDRASLRSVETQAGIPASIRTLPAGA
                     AGLLVEFQSAQEAERKQLEQLARDAVGGLKLQEAATFTHVAAEQALLWKIRAGMFPSV
                     GSVRKTGTTVIIEDVAFPIERLADAALDLNKLFVKHEYDNAIIFGHAKDGNLHFVITQ
                     GFNDQAAIDQYARFIDDVVEVVVHRYDGALKAEHGTGRNMAPFVETEWGSEGYEIMRR
                     LKQLADPHNLLNPGVIINSDPRAHLEDLKRIPTIEPEVDKCIECGYCESKCPSRELTL
                     TPRQRIVVRREMARLQDGKESSELYTALDREFPYAALDTCATDGLCATACPVSIDTGQ
                     LVKRFRRMRQTPAAQEWGVRIANHFKLTESVLRWALHGGHLMQSLIGADGMRSLTRAM
                     KRFVGDNMAEWMPDTPYAAGAVPSTARTGAQAVYFPACISRVMGRLPREPEDRSLMEV
                     TVELARRAGIPVYIPSDVAGTCCGTPFSSKGLSRAHEIAINAAVDRCWRWSEQGRLPI
                     VIDTSPCTYGFRTARGYLTEENQQRFDKMKFLDSIEFVHDELLPRLTITAKADSVALH
                     PVCSVTKLGLTPKLEAIAKACSEKVVVPLDAGCCAFAGDRGFLVPELTASATRLEAAE
                     VKAENPDACYSSSRTCEIGMTRATGQVYRSYMYLLERATR"
     gene            complement(7334..9172)
                     /locus_tag="VFC15_08655"
     CDS             complement(7334..9172)
                     /locus_tag="VFC15_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZM10265.1"
                     /translation="MTGPSATSKPSPPAARHAAPSASVGWAGYLLILLAIGVSIVVLR
                     LPELTSGWRQGYDPLGHWLLSTVIASLPVVVLLGALAFGHTKAHYAALAGLVTALLTA
                     IVGFHMPARMAAATTVYGLGYGLFPIGWIVLNVIFLYQLTVEAGRFEVLKHSLTGITQ
                     DRRLQLLLIAFCFGAFFEGAAGFGTPVAVTAALLIGLGFRPLQASGLSLIANTAPVAF
                     GALGTPIIALAGVTGFSEVTLGAMVGRILALFALMVPFWLIWAFTGFSEMLEVWPAVL
                     VAGASFATTEFLVSNYHGPWLAGVISAVVSMICLIAFLQVWHPKRVWTFEGEEQKTDH
                     RAAHGFSRGEVARAWLPWLILSVIVFCWGTVTVKKMLNTPEKVFTSMATWSTPPSKIT
                     SPAFPVHGLNKLVERTPPVVIKNTKEPAVFNFNWLSATGTGIFIAAILSGLIMGFSVG
                     QIFKIYLKTIVRVRFSLVTISAMMAVGFLTRYAGLDATMGLAFARTGHLYPFFGTLLG
                     WMGVALTGSDTSSNVLFGSLQKISAQQVGVSPVLMASANSAGGVMGKMIDAQSIVVAS
                     TATEWYGHEGDILRYVFFHSIALACLVGIVVFLMAYVAPFTHFVLQ"
     gene            9660..10169
                     /locus_tag="VFC15_08660"
     CDS             9660..10169
                     /locus_tag="VFC15_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZM10266.1"
                     /translation="MKTKLMLVASVLALLLCGAAMIAYAQGPGPEAMGGPHGMGGPGH
                     GMGFMARELNLTAAQKAQVKTIMQANHANMKPVMQQLEQNRQALLAATANGAYDPAKI
                     QLLANQKAQLEAAMTVQHEALQHQIYTQVLTSEQQAKAEQLRTQEINRITEHLQKMAN
                     GTDAPPPSE"
     gene            10363..12174
                     /locus_tag="VFC15_08665"
     CDS             10363..12174
                     /locus_tag="VFC15_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HZM10267.1"
                     /translation="MVSISSLDLITPHPKGTRARILLSSVFGPYAQDDQFGSRAINPM
                     ELYHNQVTREQGSFSLRMFHRSWGLMMIQKNISAPCTLLDFPTLDAFERELTTNHYDI
                     VGISGIIPNFAKVREMCRRVRQLSPKSTIIVGGHVAGIPGVEDMLDADHIVRGEGIAW
                     MRHYLGEDTCAPFVHPEILSGFGFRILGMSVPDNTRDTAATIVPSVGCPMGCNFCTTS
                     AFFGGKGKSFHFYETGDQLFDVMQEMERKLGVQSFFVMDENFLLNRPRAMRLLERMKA
                     ANKAWALYVFSSINAIRKYTIEELVQLGVSWIWVGLESPKSSYAKLKDADTRAIAAEL
                     RQHGIKLLGSTIVGLEHHTPENIEDEIKYAVSHCTDFHQFMLYTPVPGTPLFFQMEAE
                     GRMLDGIDLADIHGQFKFNFQHAAISRDDSKRYLDWAFRYDFEKNGPSLYRICETLFN
                     GWKRYHDHPDARIRARFAHEARKLRTTYNAALWAMEKRLRRSNPAIAARMRALRREIE
                     SAFGLSTRLMRMVVGPVLFLTSKREDRRLAKGLTFEPPSFVERSNWVETEWQAHAWRA
                     DAKNIACLKPRVSVAAVDTIAAQVGLPEEAALTVSGD"
     gene            complement(12243..13745)
                     /locus_tag="VFC15_08670"
     CDS             complement(12243..13745)
                     /locus_tag="VFC15_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM10268.1"
                     /translation="MATATSTATMHSRVEDFLSKPRPMFINNQFVNSASGKTFPTYNP
                     ATGEVLAKVAEGDREDIDRAVKAARKAFEGDAWSYMSASQRGRLIWKLADLLEQRAEE
                     FAQLESLDNGKPLAVARVADLPLAVDLFRYMAGWATKIEGNTIPISSGSKKVQFLAYT
                     RREPVGVVGQIIPWNFPLLMAAWKLGPALATGCTVVLKPAEQTPLSALLLAELIAEAG
                     FPEGVVNVVPGYGETAGAALAAHPDVDKVAFTGSTEVGKLILQAAAGNLKKVSLELGG
                     KSPNIVFADANMRSTIAGAASAIFFNHGQCCCAGSRLYVEKDMFDQVVEGVAEQAKKI
                     KVGPGFEPDTSMGPLVSLEQFNRVCGYLESGLSEGAEAVVGGKKKEGPGYFVEPTVMV
                     NTNPNMKVVREEIFGPVVVASPFSDIDQIAAEANNSEYGLAAGVWTNDINKAHRVAHK
                     LRAGTVWINCYNIFDAALPFGGYKQSGWGREMGHEVLEMYTEVKAICAAI"
     gene            complement(13962..14996)
                     /locus_tag="VFC15_08675"
     CDS             complement(13962..14996)
                     /locus_tag="VFC15_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HZM10269.1"
                     /translation="MERNPADSGEQAAFDVVVIGAGPTGLACGIDAKRAGFTVTNIDK
                     GCLVNSLYNYPSNMTFFTTPELLEIGDIPFSSPNQKPTRQEALEYYRKVTEHYQLDVR
                     QYEPLRKIEGSDNSFVVHTTDLHGRNCAYRARKLILATGYYDRPNLMNVPGEELPKVL
                     HYYREPHPFFGLDVLIVGGKNSAAIAALELWRHGARVTMVHRHEGIGTSVKYWIKPDI
                     ENRIKAGQVKAYFSSTVKEIASDHVVLATPEGEKTLANDWVFAMTGYHPDFDFLRGLG
                     IQIRGDADKKPVVNSKTLESNVPGIYLAGVLIAGMRTSEIFIENGRFHGQAIAADLKE
                     KLGPEAVTVS"
     gene            15120..16637
                     /locus_tag="VFC15_08680"
     CDS             15120..16637
                     /locus_tag="VFC15_08680"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZM10270.1"
                     /translation="MTIIKQDDLIQSVADALQFISYYHPVDFIQALTRAYEKEQSPAA
                     RDALAQLLINSRMCAEGHRPICQDTGIVSVFLKVGMDVQWDATMSVEDMVNEGVRRAY
                     LHDDNKLRASVLADPAGARKNTKDNTPAVVNMTIVPGDKLDVIVAAKGGGSEAKSKFV
                     MLNPSDSIVEWVLKTVPIMGAGWCPPGILGIGIGGTAEKAMLLAKEALMEPIDIQELI
                     ERGPSNRAEELRLEIYQKVNALGIGAQGLGGLTTVLDVKVKDYPTHAANLPIAMIPNC
                     AATRHAHFVLDGSGPVALEPPSLDDWPQLTYDPVGSGARRINLDTVTRAEVATWKLGE
                     VLLLNGKLLTGRDAAHQRMTEMLNRGETLPVDFTDRFIYYVGPVDPVRDEVVGPAGPT
                     TATRMDKFTRQMLKETGLLGMVGKSERGPEGIAAIRDHKAVYLMAVGGAAYLVSKAIK
                     KSRVLGFADLGMEAIYEFEVQDMPVTVAVDVNGNSVHDSGPREWQARIAELVEIS"
     gene            16734..16985
                     /locus_tag="VFC15_08685"
     CDS             16734..16985
                     /locus_tag="VFC15_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM10271.1"
                     /translation="MAEETRMRVSQNGRIVIPASYRKALGIDVGDEVVLRLQDNELRI
                     TTQQRRIQRAQKRARRYLKPGESLVDELLAERREAAKRE"
     gene            16978..17364
                     /locus_tag="VFC15_08690"
     CDS             16978..17364
                     /locus_tag="VFC15_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM10272.1"
                     /translation="MSRIVLDASAVLAVIQAEPGHERLTPELLSDAVCSTVNLAEVHA
                     KLVSRGLTPEEAWEDATSPAAESVPFDSEQARISGDLITQSKPLGLSLGDRACLALAI
                     ALDAPVYTAERAWKKLKTGVPIHLIR"
     gene            17496..18935
                     /locus_tag="VFC15_08695"
     CDS             17496..18935
                     /locus_tag="VFC15_08695"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZM10273.1"
                     /translation="MSKPSTRTEKDSLGTREIPADVHYGIQTARAVENFPISGLHAHP
                     SLIRAIAMVKEAAAEANRELGLVDAKTADAIITAAREVQQGKWDHAFVVDVFQAGAGV
                     SFHMNTNEVIANRASQILGGELGEYKHAHPNDHVNYGQSTNDVFPTAMRLATLLELQK
                     LYAALDGLVAAFNDKGKEFHHILKSGRTHMMDAVPMRLGQEFTAYSGAIARAKKAIEQ
                     QSELLRELGLGGSAVGTGINTHPDYRKKAVANLARISGQKLTPVDDMRYAMQSNLAMA
                     SVSSALRNLALEVVRISNDLRLLSSGPNTGLAEIILPALQPGSSIMPGKINPVMAELA
                     AMVSFQVIGYDTAVALAVQAGQLELNVMMPTMAHSVLTSITIMTNMLRQLTEFCIKGI
                     TANQKRCEFYAQSTVSLATALNPYIGYARAAEIAKESVSTGKTIIEIARSKGYLSDEQ
                     IKEILDPARMTEPQRPLDAAKDRDKLKAV"
     gene            complement(18936..19340)
                     /locus_tag="VFC15_08700"
     CDS             complement(18936..19340)
                     /locus_tag="VFC15_08700"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM10274.1"
                     /translation="MEVSVEHLGAVQFEIKARQHTVICDQPLESKGFDEGMTPPELLL
                     ASLGSCAAYYAVDYLKRNDLRQEGCRVRVTADKVKAPSRLDNFQIRVEVSDELNAGQL
                     KGVEEAVHRCLIHNTLLHPPTMNVEITTAVAV"
BASE COUNT         4149 a         5951 c         5440 g         3897 t
ORIGIN      
        1 ggctacaggc gcgggcgctt tcttcggcgg cggcgttggt gatttagacg ctcctttgaa
       61 ctcgaccgtc tgcgtcaatg ctatgttctt tttgtctttg tcaaccaatg tcagcgttaa
      121 ggttgcagag gcggcgccga acttcttcat cgatgtggcg tcgaagttaa agctgatctg
      181 cgtatcagtc ggaactggta acaaggcttc gtctatggac gcggtgacac cattcgcact
      241 taatgtaagg ctcttaattg tcgaaaggtg attccccgtc acagtcacag tctgagaggc
      301 ttgagaattc aggtcgaccg aagatggcga cactccgctc acggagaggc caatgtccag
      361 aagcacagat tgcgatgtct tctgctcaac tccgtcactt gtgactgcaa agacagtgta
      421 tgcaggactg ttaatgtagc gcaacggtgg tgaagtctac cgttgccttt gttggatctc
      481 cgctggccgg agcgtgtcaa cgactttgag acacctgccg gcgtaaatta gtgagcattg
      541 ttctcactcg tcggcgtcag agttggcgga ttgatccagg ccgccgttgg cggcgactgg
      601 ggtgtggggc aggcccgcac gaagcgttcc gggttgcgga tataggctcg gttgagaacc
      661 tcttgccgtt gctggattac ctcctgcgcc cgtccatagt gcagcatttc tggggttagc
      721 atggcgatgc cgctgtgatg gtgctcgccg ttataccagc gaaaaaagtg ctgacagaag
      781 gcgcgtgcgt cctggatcga gccgaaacga tcgggaaact ccggccggta cttgagggtt
      841 ttgaattgac tttccgagta gggattgtca tccgagacat gcggccgact gtgcgtcttg
      901 gtgactccca gatctgccag cagaaaagcc accggcttgg aggtcatcgc ggttccgcga
      961 tcggcgtgga ggttgagctg gccggccacg atgagctgct tgtcgcaggt gttgccgatc
     1021 aagcgctggg cgagttgggc gctttcgcca tcggctacca tccagccgac gacatagcgg
     1081 ctgaagatgt ccaggatcac gtagagatag aaataggtcc acttcaccgg ccccagcagc
     1141 ttggtgatgt cccaactcca gacttggttg ggagccgtgg ccagcaactc aggcttctga
     1201 taggcgggat gacggagctg gttgcgacgt tcccgcactt cgcagttttc ggccaggatc
     1261 cggtacatcg tgctgagcga acacagaaaa acgccttcat ccagcagggt ggtaaagacc
     1321 tccgccggag ccttgtcccg aaaacgttcg gcatgcagtt gatccagcac cgcctgccgc
     1381 tcggtcggcg gcagagcgcg agccggggtg ggccgctggg ccggcaaggc gaccggttgc
     1441 cggctgcgat accaggatgc ccggggcacc cccagagcac gacaggcagc cgcctgacca
     1501 acaccgaccg ccaactgctg gatggcgctc attcgttctc ctcgtcgatc ggggtatctg
     1561 gaatcggtct ccccagcagg gctgcaagtt ttttttggac atcgatgatg agttcggcgt
     1621 ttcccaggcg tttggtcaga cgagcgttgg ccgctatcag cttccgattc tcctccacca
     1681 gcgggctcac ggtggtcttg cggccccgct tcttgggggt cagggcggcc agctccccct
     1741 gccgcgcctg gcgccgccaa gtggacagat gggaagaata caatccctcc cggcgcaaca
     1801 gtgcgccgat agcgccggca tcctggcaag cgtcggattg ctcgacaatg gagcgcttgt
     1861 aatccgccgt gaaccggcgc cgtgcgggct tggcagccac ctcgggatcc ggaatggagc
     1921 cgacaggcac cccttgctgg gcgcccccgg agttagaatc tcctccgcta cgagagggct
     1981 cgctacgctc accatctctc cgctccggag atcccaactc cgcagcattc cactccaatc
     2041 gtttaacgct agtcttgctc atgaaggcga gaattctttc tccgccccta cagtaattgt
     2101 tgatggcgta gatgtctcac tattgttgac acagaggggg cggccccatt cagcgtaagg
     2161 atcccgttcg gaatatccgg gaaactgttc gtattaacgc gcatatggaa actctccaat
     2221 agcatgtacc ccgtgattgc aaacgtcccg atcagcacta ccctgcccgc gatttcggcg
     2281 ggattgcttg tttcgttggc gataactcga ggaaaagcga aacgtataag tagcacaaca
     2341 gcaatgatgt acaaggtggt gaaggtgagg tcgaatattg gattgatcat tattatttag
     2401 ctcctcaatc gaacgtctgc ttcgattgtc gccggcatac aagataaccc gcttcggacg
     2461 tttcagcgca caaatattcc cttggttggg accaaccaac tcaccatcaa acgtaaccga
     2521 gcccatcggg gagctttccc ggaaggtggc ccggccaacc atcttcacgc gaacaacctc
     2581 cttcaacatg gggtgccccg ttctagcccg ggtttggcta gggcggggcc cttcttgtcg
     2641 tggtcaccat ccatcgttgc tagcccaacg ccgctcttca ctcacccatc gacgattctc
     2701 tcggttcatg ctctaatgca ctgcgcgaga tttcgtgccg agattcccga tgcgccacaa
     2761 tatcgccgcg ttcgttatct gctgcagcac tcttttctgc gcagcccaag aaaaacccgc
     2821 cgtctccccc gagaccgtga actcagtcct cgcccaactt gatccctaca tccgctccag
     2881 cctcgctact acgaaagtcc ccggagtgtc catcgccgtc atttacaacg atcaagtcgt
     2941 attccttcgc ggctacggta ttcgaaaggt cggggaatcc gcgcaggtcg atcccgacac
     3001 cgtttttgag atcgcttcgt tctccaaacc catcacgtcg accatccttg cttctttagt
     3061 aggcgaaggc aaaatcagct gggacgaccg catcgcggac atcgatcccg gcttccagct
     3121 ttccagcccc gacaccaccc gccagatcac catccgcgac ttcctctccc accgcagcgg
     3181 cctcgccacc gaatccggcg acctgctgga agacctcggc tattcccgcc ccatcatcct
     3241 ttatcgcatg cgttatctgc cgctgccggg acaattccgc aaaacctatg cctacagcaa
     3301 tttcggatac accactggag ccatcgccgc tgccaccaag atcggcaagc cctgggagac
     3361 gattgccgaa gagcagctct actctcgcct cggtatgacc tcgaccagtt cgcgcttctc
     3421 cgactacgaa gaccgcgcca acaaagccgc actgcacatt ttcatcaacg gcgagcccgt
     3481 caaccgtttc gtgcgcgagg ccgacgccga agctccagcc ggaggagtct cgtccagcgc
     3541 ccgcgatctc gccgagtggg tgcggttgca actcagtggc ggcaaatgga acagcaagca
     3601 gatcatcgac gccaacgccc tcgccgaaac ccacaaagcc cagatctgcc gcaatcctgc
     3661 tgacccggca aagcccaatg tctgtcccgg cgaccaatac tacggcctcg gatgggacgt
     3721 cggaaccaat gccgagggcc acacccaggt cagccatagc ggcgcgttct tcctcggcgc
     3781 tgccactgcc gtctacctgg tccccgaaga gcacatcggc gtgctcgccc tcagtaacag
     3841 cctgcccgtc ggtttgccgg agtcaatctg cctgcacttc ctcgatatcg tgcactacgg
     3901 aaaaccgcag cgtgactacc tttcgctcgc cgccaagctc ttctcccaaa tgatcgccca
     3961 gactcaggat gcatctcccg actacgctgc gatgacgcag cccaagacct cctctccggc
     4021 ccagccgctc gccgcgtatg ccggcaagta caccaatgaa tacttcggaa ccctggaagt
     4081 ctcggtcgag aacaatcaac tggtcctgcg acttcccccg cgcggctcct actacgaact
     4141 tgctcactgg gacggagaca cgttcaccta ctacttcgcc agcgaaaaca ccggcgccgg
     4201 tcgccgcggg gcaatattct cgccagaaaa gaaccaggtg ctgatcgaaa tgctctcgcc
     4261 ggagcacaac gcggtgtttg ccaggtcgca gcctgagcat taaaagacca ggcctggctg
     4321 gggtcagatt attcgaactt gaaacttgaa actgacttgc taccgcgtcg cccgttccag
     4381 caggtacatg tacgaccgat agacttgccc cgtcgcccgc gtcattccga tctcgcaggt
     4441 tcggctgctg gaatagcatg catcgggatt ctcggccttc acttccgccg cttccagcct
     4501 cgtggccgaa gcagtcagct caggcaccag aaatccgcgg tctccggcaa atgcgcaaca
     4561 gccggcatct agcggaacga ccaccttctc actgcaggct ttggcaatgg cttccagctt
     4621 cggagtcagc cccagcttgg tgaccgagca taccggatgc agcgccaccg agtccgcttt
     4681 cgccgtgatg gtcagcctag gcagcagctc gtcgtgaacg aactcgatgg aatccaggaa
     4741 cttcatcttg tcgaatcgct gctggttctc ttccgtcaga tatccgcgtg ccgttctgaa
     4801 cccatacgtg caaggactgg tgtcgatcac aatgggcagt cttccctgct ccgaccacct
     4861 ccagcaacgg tcgaccgcgg cgttgatcgc aatctcgtgc gcccggctga gtcccttgga
     4921 ggagaacggc gtcccgcaac acgttcccgc aacgtcgctg ggaatgtaca ccggaattcc
     4981 agcgcgccgc gccagctcca cggtcacttc catgagcgag cggtcttcag gctctctggg
     5041 taaccggccc atcactcgcg aaatgcacgc cggaaaataa actgcctgcg ctcccgtgcg
     5101 cgcagtcgaa ggcacggcac cagcggcgta cggagtgtcg ggcatccatt ccgccatgtt
     5161 gtcgccgaca aaacgtttca tggcacgcgt caaagagcgc attccgtccg cgccgatcaa
     5221 actctgcatg agatggccgc cgtgcagcgc ccaccgtaaa acggattccg tcagcttgaa
     5281 gtgattggca attcgcacgc cccattcctg cgcggccggc gtttgccgca tgcgacggaa
     5341 gcgtttcacc agttgcccag tatcgatgct taccggacaa gctgttgcgc acaatccatc
     5401 cgtcgcgcaa gtgtcgagcg cagcgtacgg gaattcgcgg tcgagcgccg tgtacaactc
     5461 tgatgattct ttcccgtcct gcaatcgcgc catctcgcgg cgcacgacaa tccgctgccg
     5521 tggcgtcagc gttaattcgc ggctcggaca cttcgactcg caatagccgc actcgatgca
     5581 cttatcgact tcgggctcaa tcgtgggaat gcgcttcaga tcttcaaggt gcgcgcgcgg
     5641 atcggagttg atgatcacgc cgggattaag caggttgtgc ggatcggcca attgcttcag
     5701 ccgccgcatg atttcgtagc cttcgcttcc ccactcagtc tcgacaaacg gagccatgtt
     5761 gcggcccgtc ccgtgctcag ccttcaacgc gccgtcgtag cgatgcacca cgacctccac
     5821 cacgtcgtcg ataaaacgcg cgtactgatc gatagcagcc tggtcgttga aaccttgtgt
     5881 aatcacgaag tgcaggttgc cgtctttggc gtgtccgaag atgatggcat tgtcgtattc
     5941 gtgcttgacg aaaagcttat tcaggtcgag cgcagcatca gccagccgct caataggaaa
     6001 tgcgacatcc tcgatgatga cggtggttcc agttttgcgt accgatccca cggaaggaaa
     6061 catgccggcg cgaatcttcc acagcagcgc ctgctctgcg gcgacgtgag taaacgttgc
     6121 ggcttcctgc agcttcaaac caccaaccgc atcgcgcgcg agttgttcga gttgcttgcg
     6181 ttcggcttcc tgggctgatt gaaactccac cagcaatccc gcagcgcctg cgggcaacgt
     6241 gcgaatcgat gccggaattc ccgcctgggt ttcgaccgag cgcagcgacg cgcgatccat
     6301 cacttcaagc gccttcgcac ccgcctggcg cagaggaacg atggagtccg ctgccgcata
     6361 caaatcaggg aacaacagca acccggtata tttcacggga agatcaggca ccgtatcgag
     6421 caccgcctcc gcgataaagg cgagcgtgcc ttcggcgccg atgaggacgt gctggaagat
     6481 gtccacggcc gagtcgaaat cgagaaaagc gttgagcgaa tagcccgtgg tgttcttggt
     6541 gcggtacttg cgacgcacgc gctcgcgcag atacgaatcg ctctcgagat gcgccttcaa
     6601 atcgaggatg ccacgcgcca actccggttc gcgagcatga aattgctgtt ccgcatccgg
     6661 ttgcgccgcg tcgatgacgg tcccagacgg cagcatgaac ttcagagagt gcagcgtgtg
     6721 ataggcgttc tgctccacac cgcaacacat gccgctggag ttgttggaga gaatccctcc
     6781 caacgtacaa gtggcgatag aagcgggatc gggaccaatc ttggcgccat aaggagcaag
     6841 aagctggttg gcgcgcgcgc caatcactcc cggctgcacg cgaatcttct tgccgccatc
     6901 ttcgacgttc agcccacgcc aatggcgtgc gacctcaacc agcagaccat ccgagatcga
     6961 ttggcccgac aggctcgagc ccgcagcgcg gaaggtcatg gggattcccg agtgctggct
     7021 gaattcgaag agcgtcttga tttcctcaag gctcttcgtc agcacgactg ctttggggat
     7081 gaggcgatag aagctggcgt cggaggcgaa ggcgatgagg tcgatgggac gaactagaat
     7141 tcgatcgcca tcgatttgcg aggcgagcga cgatttaatt tcgtcggaag aaaccggagc
     7201 agatgtgacg acagctacag ccatgatgat cgattcccta cccctaaatc tcccagtgag
     7261 catcagaatc gatgcccacc gggaattcaa tcattgtctt cgaacgactt tcagagcgta
     7321 ccctgccccc gctctattgc aatacgaagt gcgtgaacgg agcaacataa gccatgagaa
     7381 acaccacaat ccccaccaga cacgccaacg cgatgctgtg gaaaaagaca tagcgcaaaa
     7441 tgtcgccctc gtgcccatac cactcggtag cggtactggc gacgacaatg ctttgcgcgt
     7501 caatcatctt gcccatgacg ccgccggcac tgttggcgga cgccatgaga acaggcgaaa
     7561 cgcccacctg ctgggccgaa atcttctgca gactgccgaa gagcacgttg gacgaggtat
     7621 cagatccggt cagtgcgact cccatccatc ccagcaaggt tccgaagaac ggatagaggt
     7681 ggccggtgcg ggcgaaggcc agtcccatgg tcgcgtcgag ccctgcgtaa cgcgtcagga
     7741 agccgacagc catcatggcg gaaatcgtca ccaacgaaaa tctcacgcgc acgatggtct
     7801 tgagatagat cttgaatatc tgcccgacgc tgaaacccat gatcagtcca gagaggatcg
     7861 cggcgataaa gatgcccgtt ccagtggcac tgagccaatt gaagttaaat accgccggct
     7921 cttttgtgtt cttaataacc accggaggcg tgcgctccac cagtttgttt aagccatgaa
     7981 ccgggaatgc cgggctggtg atcttactgg ggggcgtact ccaagtggcc atcgaggtga
     8041 aaaccttttc cggggtattc agcattttct tgacggtaac agtcccccag caaaaaacga
     8101 tgacgctgag aatgagccag ggcagccagg cgcgagcgac ttctccgcgg ctaaagccgt
     8161 gggcggcgcg atggtcggtc ttctgttctt cgccttcgaa ggtccacaca cgcttggggt
     8221 gccacacctg tagaaacgca atcagacaga tcatggaaac cacggcggaa atcacgcctg
     8281 cgagccacgg accgtgataa ttggaaacca ggaactcggt ggttgcaaag gaagcgcctg
     8341 cgaccaacac ggcgggccag acttcgagca tctccgagaa cccggtgaag gcccagatca
     8401 gccagaatgg aaccatcaac gcgaacagtg ccagaatgcg acccaccatc gcgcccaggg
     8461 tgacctcgct aaatccggtg acgccggcca gcgcgatgat gggcgttccc agtgcgccga
     8521 aggcgaccgg cgcggtgttt gcgatcagcg ataacccgga agcctgcagg ggacgaaagc
     8581 ccaaaccaat gagaagagcg gcagtgaccg cgacaggggt gccgaacccc gcagcgcctt
     8641 cgaaaaatgc gccgaaacag aaagcgatga gaagaagttg caggcggcgg tcctgagtga
     8701 tgccggtcag gctatgcttc aacacttcga agcgcccggc ttcgacagtt aactgataca
     8761 ggaagatgac gttcagcacg atccagccga tgggaaacag tccgtatccc aagccgtata
     8821 ccgtagtggc ggccgccatc cgtgccggca tgtggaatcc cacaatcgcg gtcagcagcg
     8881 cagtcacaag tccggcgaga gctgcatagt gggccttggt atggccaaac gcgagagcgc
     8941 cgagcagcac aaccaccggc agcgaagcga tgacggtgga caacaaccag tgtcccaagg
     9001 gatcgtatcc ctgccgccag cccgaagtga gctcgggcaa acgcagcacc acgatggaaa
     9061 ccccaatcgc caacagaata agaaggtaac ccgcccagcc taccgatgca gacggagcgg
     9121 cgtgacgcgc tgcgggtggg gacggctttg aggttgcgga aggacctgtc atgcgtgcgc
     9181 ctcctggaag aacgctcaat tcaatgcgag tttgtggagc tgaaatgtcg tccgaatctt
     9241 actcgcgaaa ctttaagata gccaacccta agtgctgcat gacttggctc actggccgtt
     9301 gtgacgtacg gcacaatttc gagcaaagca gcccaatgtg cagcctgctc cgcaaacgtg
     9361 ggacgaaccg cagtcaagac cgtagagtgc gccccagcga aacgatgcac cagacgtaag
     9421 agtccgcacg gatgcctttg agattctgga actttcccct cgcgcggcgg gaaacccata
     9481 cgaagaacaa gacaccaagc gtcaggaaat gcgcggacct gtggcctcca aaaccggtta
     9541 accgctcgtg gagtgctaca agtctttacc tcgcgttacc agactttacc gtcgaagctg
     9601 tgcgcctagt gttcctagaa gtgacagaca aactgtcgaa accaagagga gttgaaggca
     9661 tgaagacgaa gttgatgtta gttgcaagcg tattggcgtt gctgttatgt ggcgccgcga
     9721 tgatcgccta cgcccaggga ccagggcctg aagcgatggg tggtccgcac ggcatgggcg
     9781 gtcccggcca cggcatggga tttatggctc gcgagctgaa cctcaccgct gctcagaagg
     9841 cgcaggtcaa gacgatcatg caggcaaatc acgccaacat gaagcctgtg atgcagcaat
     9901 tagaacagaa tcgccaggca cttctggccg cgacagccaa tggggcatac gatccggcga
     9961 aaattcagct actggccaac cagaaggcac agttagaggc ggcgatgacc gtccagcatg
    10021 aggcgctgca gcaccagatt tacacccagg tgctgacctc tgagcagcag gcgaaagccg
    10081 agcagcttcg cacgcaggaa atcaatcgca tcactgagca cctgcagaag atggccaacg
    10141 gaaccgacgc tcccccgccg tctgaatagc gattcgcaaa aagaatgtcc gggcaggatt
    10201 tcggtcctgc cctttttgtt gctcgtgcgt tgatgacgtc gcagccagaa acaattgttg
    10261 cgagtttctt cccggcagag tgctgtgtaa tagttttgtc atatcagttg gtttgcgtca
    10321 tttgtcacgg acatcgtgct gccatcttcc tacgatggaa tcatggtttc catatcaagc
    10381 ctcgatttaa ttactccgca tcccaaggga actcgggcac gcattctgct gagttccgtg
    10441 ttcggcccct acgcgcaaga cgatcagttc ggcagccgcg ccatcaatcc gatggagctc
    10501 taccacaacc aggttacccg cgagcaaggc agcttttctt tgcgcatgtt ccaccgctct
    10561 tggggcttga tgatgatcca gaaaaacatc tcggctccat gcactctgct cgacttcccc
    10621 acccttgacg cttttgaacg cgaactcacc accaaccact acgacatcgt cgggatctcc
    10681 ggcatcattc ccaacttcgc caaggtacgc gagatgtgcc gtcgcgtgcg gcaactctcg
    10741 cccaagtcca ccatcatcgt gggaggccac gtggccggca tccccggagt ggaagacatg
    10801 ctcgatgccg atcacatcgt gcgcggcgaa ggcatcgcat ggatgcggca ctacctcggc
    10861 gaggatactt gcgcgccgtt tgttcatcct gaaattctgt ctggcttcgg cttccgcatt
    10921 ctgggcatga gcgtgccaga caacacgcgc gataccgccg ccaccattgt tccctcggtc
    10981 ggatgtccga tgggctgcaa cttctgcacc acgtccgcct tcttcggagg caagggcaaa
    11041 agcttccact tttatgaaac tggcgatcag ctcttcgacg tcatgcaaga gatggaacgc
    11101 aaactgggcg tgcagtcgtt ctttgtcatg gacgagaact tcctgctcaa tcgtccacgc
    11161 gccatgcgcc tgttggagcg catgaaagcc gccaataaag cctgggcgct ctatgtcttc
    11221 tcgtcaatca acgccattcg gaaatacacc atcgaggaat tggtgcagct tggagtgtcg
    11281 tggatctggg tcggactgga gtcgcccaaa tcgagttatg cgaagttgaa agacgccgac
    11341 acccgcgcga tcgccgcgga actgcggcag cacggcatca agctcctggg ctctacgatc
    11401 gtgggcctgg agcatcacac tcccgaaaat atcgaagacg aaatcaagta cgcggtttcg
    11461 cactgtaccg acttccatca gttcatgctg tatacgccgg taccgggcac gccgctgttc
    11521 ttccagatgg aagcggaagg gcgcatgctc gacggtatcg atctcgccga cattcacggc
    11581 cagttcaagt tcaactttca gcacgccgcc atctcgcgcg acgattcgaa gcgctacctg
    11641 gactgggcct tccgctacga cttcgagaag aacggtccga gcctgtaccg catttgcgag
    11701 accctgttta acggatggaa gcggtatcac gaccatcctg acgcacgcat acgcgcacgc
    11761 ttcgcgcacg aagcacgcaa gcttcgcacc acttacaacg cagccttatg ggcgatggag
    11821 aagcggctac ggcgcagcaa tcctgcaatc gccgcacgga tgcgcgcatt gcggcgcgag
    11881 atcgaatccg cgtttggcct ctccacgcgc ctgatgcgca tggtcgttgg cccagtgttg
    11941 ttcctgacat cgaagcgcga agatcgtcgt ctcgccaagg gcctgacctt cgagccgcca
    12001 tcattcgtcg agcgcagtaa ttgggttgaa acggaatggc aggctcacgc ttggcgggct
    12061 gatgcgaaaa acattgcgtg cctgaagccg cgtgtgtcag tggctgcggt cgacacgatt
    12121 gcggcgcagg ttgggttacc cgaagaagct gcgttaactg taagtggtga ttaattagcc
    12181 gagcagaaaa atgcggtact gtcgcttcgc gcatagtcaa tagcgacagt accgcagtgt
    12241 tgttagatcg cggcgcagat ggcctttact tcggtgtaca tctccaatac ttcgtggccc
    12301 atttcgcggc cccaaccgga ctgcttgtat ccaccgaacg gcagcgctgc gtcaaatatg
    12361 ttgtagcagt tgatccaaac agtcccggcg cgcaacttgt gcgccacgcg gtgcgcctta
    12421 ttgatgtcgt tcgtccagac tccggcggcc aggccgtatt cgctgttgtt agcttcggcc
    12481 gcgatctggt cgatgtcgct gaacggcgac gccaccacca ccggcccaaa aatctcctcg
    12541 cggacgacct tcatattcgg gttcgtattg accatcacag tgggctcgac gaaataaccc
    12601 ggtccttctt tcttcttgcc gccgaccaca gcttccgcgc cctcgctgag tccggactcg
    12661 agatagccgc aaacccggtt gaactgttcg agcgacacca gcggacccat gctcgtatcg
    12721 ggctcaaatc ccggaccgac tttgatcttc ttggcctgct ctgcaacccc ttcaaccacc
    12781 tggtcgaaca tgtccttctc aacgtagaga cgcgaacccg cgcagcagca ctgtccgtga
    12841 ttgaagaaga tcgcgctcgc cgcaccggca atcgtggaac gcatgttggc gtcggcgaat
    12901 acgatgttgg gcgacttccc gcccagctcc agcgatacct ttttcaggtt gcccgccgcg
    12961 gcttgcagga tcagcttgcc cacttccgtc gatccggtga atgccacctt atcgacatcg
    13021 ggatgcgcag ccaatgcagc gcccgccgtc tcgccatagc ccggaacgac gttgaccacg
    13081 ccctccggga atccggcttc ggcaatcaac tcggccagca acagcgccga tagcggagtc
    13141 tgctccgcgg gcttcagcac cacggtgcat cctgtcgcca gcgctgggcc caacttccag
    13201 gcggccatca agagcgggaa attccacggg ataatctgcc ccaccacgcc taccggttcg
    13261 cggcgagtgt aggcaagaaa ctgcaccttt ttcgagccgg acgaaatcgg gatcgtgttg
    13321 ccttcaatct tggtggccca tcccgccatg tagcggaaca ggtcaaccgc aagcggcaga
    13381 tcggcaacgc gtgccaccgc cagaggctta ccgttgtcga ggctctccag ttgtgcgaac
    13441 tcttcggcac gctgctccag caggtcggct aacttccaga tcaagcggcc gcgctgcgag
    13501 gccgacatgt aagaccatgc gtcgccctca aaggctttcc gcgctgcctt cactgcgcga
    13561 tcgatgtcct cgcggtctcc ctctgcaacc ttcgccagca cttctcccgt tgccgggtta
    13621 taggtcggga aagtcttgcc ggaagcggaa ttcacgaact ggttgttgat gaacatcggc
    13681 cgcggcttgc tgagaaaatc ttccacgcgg ctgtgcatgg ttgcggtgct ggttgcggta
    13741 gccattgcca tcctcccacg gcgaagtgcg tcttctgcat caccccgccg gtactgaatt
    13801 ggaattctat gagtggtcaa gcggtcgctt cggcatctcg tccgaaacgt ccgagcctca
    13861 tcccgcgcaa gagtttatca gagacttggc agccgtgggt atcggaacta cggggcagat
    13921 ctcatcaaga acgaagcact cgcagcgaac agtattaggt cctaagaaac cgtgactgct
    13981 tccgggccca gcttctcttt caagtccgcc gcaatcgctt ggccgtgaaa gcgtccgttt
    14041 tcgatgaaga tctcactggt ccgcataccg gcaatcagca cgcccgccag gtaaatgcct
    14101 ggaacattgc tttccagcgt tttggaattg accaccggct tcttgtcggc atcgccgcga
    14161 atttgaatgc ccagcccgcg cagaaaatcg aaatcgggat ggtagccagt catggcaaac
    14221 acccagtcgt tggccagcgt cttttcgcct tcaggagtcg ccagcaccac gtgatcggaa
    14281 gcgatctcct tcaccgtgct gctgaagtag gccttcactt gtccggcctt gatgcggttt
    14341 tcgatgtccg gcttgatcca gtacttcacc gaggttccga ttccctcgtg ccggtgcacc
    14401 atcgtcaccc gcgcgccgtg acgccacagt tccaacgccg caatggccgc cgagttcttc
    14461 cccccgacga tcaagacgtc gagtccaaag aaaggatgtg gctcgcggta ataatgcagg
    14521 acttttggca gttcctcgcc ggggacattc atcaggtttg ggcgatcgta atatccagtg
    14581 gccaaaatca atttgcgcgc ccgatacgcg caattgcgcc catgcagatc ggtggtgtgc
    14641 acgacaaaac tgttgtcgct gccttcgatc ttccgcagcg gctcgtactg ccggacgtcg
    14701 agctgatagt gctcggtcac cttgcggtag tactccagcg cttcctgccg cgtaggtttc
    14761 tggtttgggc tggagaacgg aatatcacca atttccagca gttcgggcgt ggtgaagaac
    14821 gtcatgttac tgggatagtt gtagagcgag ttgaccaggc aacccttgtc gatattggtc
    14881 accgtgaacc cagcgcgctt ggcatcgatg ccacaggcca ggccagttgg gccagcgcca
    14941 attaccacca cgtcaaaagc tgcctgttcc ccggaatctg caggattgcg ttccatgtct
    15001 ctaggatacc gtgagtgaca gcacccgatc gccgcggtga cctacgtcac tggttatggg
    15061 gcctacactt ttgtatgctg ttgagtacgc caatcctgct tttttcccga gaggcggtca
    15121 tgaccatcat caagcaagac gatctcatcc agagcgtagc cgacgccctg caatttatct
    15181 cctactacca cccggtcgat ttcattcagg cgcttacgcg tgcctatgag aaagaacagt
    15241 ctccagcggc acgcgacgcc ctcgctcagt tgttgatcaa ctcgcgcatg tgcgccgagg
    15301 gccaccgccc catctgccag gacactggca tcgtcagcgt tttcctcaag gtcggaatgg
    15361 acgtccaatg ggacgcgacc atgagtgtcg aagatatggt caacgaaggc gttcgccgcg
    15421 cctatcttca tgacgacaac aagctgcggg ccagtgtgct ggccgatccc gctggcgccc
    15481 gcaagaatac caaagacaac acgcccgcag tcgtcaacat gacgatcgtt cccggcgaca
    15541 aactggacgt gatcgtggcc gccaaaggcg gaggctccga agccaagtcg aagttcgtca
    15601 tgctcaatcc ctccgactcc attgtcgaat gggtgctgaa gactgttcct atcatgggcg
    15661 cgggatggtg tccgcctggg attttgggca tcggcatcgg cggcactgcc gagaaagcca
    15721 tgctgttggc caaggaggct ctgatggagc cgatcgacat tcaggagttg attgagcgcg
    15781 ggccgtcgaa tcgcgccgag gagttgcgcc ttgagatcta ccagaaggtc aatgccctcg
    15841 gcatcggggc gcaaggactc ggcggactga ccaccgttct cgacgtcaag gtgaaagact
    15901 accccaccca cgccgcaaat ctccccatcg cgatgattcc caattgcgcc gccacgcgcc
    15961 atgctcactt cgtactcgac ggctcaggcc cggtcgcctt ggagccaccc tctttggacg
    16021 actggccgca gctcacttac gatcccgtag gatcgggcgc gcgccgcatt aatctcgaca
    16081 ccgtgacacg agccgaagtt gccacctgga aactcggcga agtactgctg ctgaatggca
    16141 agttgctcac cggccgcgat gccgcccatc agcgcatgac cgaaatgctc aaccgcggcg
    16201 agacgctgcc cgttgatttc actgatcgtt tcatctacta cgtaggcccg gtcgatcccg
    16261 tgcgggatga agtcgttggt cctgcgggtc ccaccacggc aacgcgcatg gataagttca
    16321 cccgccagat gctcaaggag actggcctgc ttggcatggt gggcaagtcg gaacgtggac
    16381 cggaaggcat cgcagccatt cgcgatcaca aggcggtgta cttgatggcg gttggcggcg
    16441 ctgcgtatct cgtctccaaa gcgatcaaga agtcgcgcgt gctgggcttc gccgatcttg
    16501 gcatggaagc catctacgaa ttcgaggtgc aggacatgcc agtcacagtc gccgtcgatg
    16561 tgaacggcaa ctcggtgcac gacagcgggc cgagagagtg gcaggccagg atcgcggagt
    16621 tggtggagat ttcataaact gaagacaggc gcagtgggca caactccatt ttctgccaat
    16681 cggcatagga ttggcaatat gatagaatat tggcagaaat acaggagttt gccatggcag
    16741 aagagacccg aatgcgtgtc agccagaacg gtagaatcgt gattcccgct tcctaccgta
    16801 aggcccttgg tatagatgtt ggagacgagg ttgtattgag gttgcaagac aacgagttgc
    16861 gtatcaccac ccagcagcgc cgcattcagc gcgcccagaa gcgtgcccgc cgctacctga
    16921 aacctggcga atcgctggtt gacgagttgc tcgccgaacg ccgcgaggcg gccaagcgtg
    16981 agtagaatcg tgctcgacgc ctccgcggtg ctggccgtga ttcaggctga gccgggacac
    17041 gaaagactca ctccggaact gctctccgac gcagtctgca gcaccgtgaa tcttgccgaa
    17101 gtccatgcga agctggtgag ccggggcctg acgcccgagg aagcgtggga agacgctacc
    17161 agccccgctg cggaatcggt gcccttcgat agcgaacaag cgagaatctc cggcgacctg
    17221 atcacccaaa gcaagcccct cggcctctcg ctcggagacc gcgcctgcct cgcgctggcg
    17281 attgcgctcg atgccccggt ttacactgcc gagcgggcct ggaaaaaact caaaaccggt
    17341 gtccccatcc atctgatccg ctaggctcgt gggggctggg agtcaacgat tccgggcgcc
    17401 gcatcctgtc gcccgccgtt cgcgacaggg agggatttgg cgacgcctga accgcagccg
    17461 cctccgctat catctctctg ctggaggcaa cgcccatgtc caagccatcc acccgaactg
    17521 aaaaagattc tctcggcacg agggaaatcc cggccgacgt tcactacggc attcaaacgg
    17581 cgcgcgcggt cgagaacttc cccatcagcg gactacacgc ccatccttcc ctgatacgag
    17641 ccattgccat ggtgaaagaa gctgcagccg aggccaaccg cgagttggga ttggttgatg
    17701 cgaaaaccgc cgacgcgatt attacagccg ctagagaggt tcagcagggc aagtgggacc
    17761 atgcgttcgt ggtcgatgtc ttccaggctg gagccggtgt tagcttccac atgaacacca
    17821 acgaggtcat tgccaaccgc gcttcgcaga ttcttggcgg cgaacttggc gagtacaagc
    17881 acgcccatcc caacgaccac gtcaactacg gacagtccac caacgacgtc ttccccaccg
    17941 ccatgcgact ggcgaccctg ctcgaattgc agaagcttta cgctgccctc gatggcctcg
    18001 tggccgcctt caatgacaag ggcaaagagt ttcaccacat cctgaagtca ggacgcaccc
    18061 acatgatgga tgccgtcccc atgcgcctgg ggcaggaatt caccgcctac tccggtgcga
    18121 tcgcgcgggc gaaaaaagcg atcgagcagc aatccgagct gctgcgtgaa ctaggcctcg
    18181 gaggctcggc cgtcggcacc ggcatcaaca cccatcccga ctatcgcaag aaggccgtcg
    18241 ccaacctcgc gcgcatctct ggccaaaagc tcacgccggt ggacgacatg cgctacgcta
    18301 tgcagtccaa tctcgcgatg gcctcagtca gttcggcgct gcgcaatctt gcgctggagg
    18361 tcgtccgcat ctccaacgac ctgcgcttgc tctcctcggg accgaacacc ggcctcgccg
    18421 aaatcattct gccggcgctg cagccgggat cgtccatcat gcccggcaag attaatcccg
    18481 tgatggccga gctagctgcg atggtttcgt tccaggtcat cggctacgac accgcagtcg
    18541 cgctcgccgt ccaggctggg cagctcgaac tcaacgtcat gatgccgacc atggcgcaca
    18601 gcgtcctcac gtcaatcacc atcatgacca acatgttgcg ccagttaacg gagttctgca
    18661 ttaagggcat cacggccaac cagaagcgtt gcgagttcta cgcccagagc acggtgtcgc
    18721 tggcgacggc gctgaatccc tatatcggtt atgcaagagc agcggagatc gcgaaagagt
    18781 cagtttcgac cggaaagacg atcatcgaga tcgctcgctc gaagggctat ctgagcgacg
    18841 agcagatcaa ggagattctc gatcccgcgc gcatgaccga accgcagcgt ccgctggacg
    18901 ccgcgaagga tcgggacaag ctgaaggccg tgtgactaaa ccgccacggc cgtcgtaatc
    18961 tccacgttca tggttggcgg atgcagcagc gtgttatgga tgaggcaacg gtgcaccgcc
    19021 tcctcaacac ctttcagctg accggcattg agttcgtcgg aaacctcgac acgaatctga
    19081 aagttgtcga gccgagaggg cgccttgact ttgtcggcgg tgacacggac cctgcagcct
    19141 tcctggcgca gatcgtttcg cttcaggtaa tccaccgcat aatacgccgc gcacgagccc
    19201 agcgacgcca gcagcaattc cggcggcgtc atcccttcgt cgaagccttt gctttctaag
    19261 ggctgatcgc aaatcaccgt atgttgtcgg gccttgattt caaattgcac tgctccaaga
    19321 tgttctaccg agacttccat gattgctcct cacttaataa aggtctctct cgcatttatc
    19381 tgggggccat cccaggtgga accagcgcct cgtccaggta tcgccgaagg tttgtcc
//
LOCUS       DASRKK010000388        26211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1398370, whole genome shotgun sequence.
ACCESSION   DASRKK010000388 DASRKK010000000
VERSION     DASRKK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 26211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26211
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398370"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            901..2508
                     /locus_tag="VFC15_08705"
     CDS             901..2508
                     /locus_tag="VFC15_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10275.1"
                     /translation="MAFCDGVPKRLVATSIALAFAVCFLSSFAAAQITLQPRAEVFGG
                     YSWYHPNGYVDWGKVPDIAHGWNVSSTFYFPNARNLGVVIDGSGHYNGTYSNSGFGLA
                     GLQYKFRNNQFSPFVRVLVGATKISPAALNSEWRATVGGGGGFDLTLSNLISLRLAQA
                     DYLYTSYNTQTFIDHNSTWNSVRLSAGVVFNLGNYYTAPLAAACSASPTTPVFAGEPV
                     TVTTTGTNFSPKHTLAYAWTATGGKLAAPSAASSAVDTTGLAAGTYTANATITDPKWK
                     KNNSASCSTSFTIKPFTPPSVTCSASVTTVKSGETATITANATSQDGAQISNYAYTTS
                     AGKITGNGTSATLDTTGLPGSTVSVTVTATDSHGLSASCTASVGVVEEIKCVSIEDWG
                     ECTFEKNPKKPWRVDNDCKDTLDKLSLRLQQMPSGKVEIVGYTDEKESVDVKQLGAQR
                     SVNVKYYLTTDGPTKSDATRIEPRDGGTKGKATHFFYLPDGALCSGQAVEGTPVDETQ
                     VQPQSRNAPAPAKKHKAKAKPAAPPAQ"
     gene            2837..4966
                     /locus_tag="VFC15_08710"
     CDS             2837..4966
                     /locus_tag="VFC15_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10276.1"
                     /translation="MRNLVGSVAKLVVGFALLFTICTVTAWASDWQLIGPDGGNVRCL
                     AYDPSDPNRILLGTSAGQLFSSRDGGNSWVPFAHLGSGDDLVIDHIVFDPTNPQTIYA
                     AAWGLYHDDEGDVFRSDDGGATWRTLAGVHGKSIRALAMAPSDHNTLVIGALDGVFRS
                     QNGGATWEKMTPENPDVLENHASMRNFVSVAVDPQNPDVVYAGTRHLPWKTSDGGKSW
                     HNLKDGILDDSDVFSIIVDPKTPSRVYASACSGIYKSDNGAELFHRVQGLPHSAIRTR
                     VLKQDPQRSSIVYAGTTGGMWKTVDGGTKWTLVTSADVVINDVLIDPRNPQRVLIATD
                     RGGVLASNDGFAKYQSSNRGFSHRMVGSVIADRNNSNRLYVGIVNDKTDGGLFITDDA
                     GKSWQQVSKGLGDRDVLSLQQAENGAIFAGTNHGIFSLATLSGAWLPATMIHGPVPEW
                     QPMAEAVPEAAAQKTLKSKTAKGTAKKPAVTAKKKEPAEVPIPIAIAPRVRSIQITNK
                     AWYAATNDGLFISVDGGKKWYGTPIDGESDFLAANDYPDGTLTLASIKRAFISRDAGR
                     TWSEIVAPTYVTGLYNVTMTQDSSLWLGAREGAIRSNDGGKSWSHVMNGLPPSYVLGV
                     KYDAKAQRLLATAMNSRGVFESVDGGKTWHQTPEASFSIRTAMGYQDLLLAISWHNGL
                     LLQRGDSTASVSNAAVTVGSAAVKSQQ"
     gene            5348..5842
                     /locus_tag="VFC15_08715"
     CDS             5348..5842
                     /locus_tag="VFC15_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZM10277.1"
                     /translation="MNAFRSADCGEKVGTNPVAAIDCNVDLCGRTRAGIPEDGCNDCG
                     AILHGLNNALAAMLLNAQVLEWKLPSYSRSRRYLHEIERNAQRGGALVKQLLGRMEAR
                     CEIGLSGCRIDGEGLTLAGDDGVASARELATVGQVEEPPLGQTARPAGVLLAHRKKVP
                     HTMV"
     gene            5954..7363
                     /locus_tag="VFC15_08720"
     CDS             5954..7363
                     /locus_tag="VFC15_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM10278.1"
                     /translation="MLTVEPVSAVEQTADTNLLNLLIVDDERSVRESCREVAVALGFN
                     TQIADSPEHCYHILDTTNVDVVLLDLRLPGTNGVEVLREIKRHRPETVVVIMTGFATV
                     QSAVQAMKAGAYDYVTKPFSFDELRLVLDRVTAHVRLTTENRLLREQVKSKHGFGAMV
                     GQAPEMDKLYRIIAKATHSTHPVLILGESGTGKELVARSIHYSGPFRDKPFIPVDCGS
                     LVATLIESELFGYVRGAFTGAVRAKDGLMAAAEGGTIFLDEIGELPLDLQSKLLRALQ
                     EKEIRPVGGTRAIPINVRILAATNRDLEAAVQDGTFRRDLYFRLNVLTLRVPPLRERK
                     QDIPLLAGHILERVARTTGVQRNISDDALKLMLNYEWPGNVRELENCLERACALTSGP
                     TMHIADLPTALQNFHTQTPMVPVSLESTGIAPLADLEKRAILHAITILNGDKLEAARQ
                     LGIGKTTLYRKLKEYGSVV"
     gene            7450..8007
                     /locus_tag="VFC15_08725"
     CDS             7450..8007
                     /locus_tag="VFC15_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10279.1"
                     /translation="MVILVTTSPRAKECATAIEQTTHQKTQIATSLARAVECLQTHDY
                     DALVLDESFNHAEIGGANLLLNHAGSAMPIYVNLALHGTERVTREVQNGMLRFVREKL
                     AAMRSAENVLRSLLRGEVTAILLNSELALREPSLSASIAEKIHIMHDLAEGMRLKLEG
                     APADGMGEHTHIPAAIAHEPRPRPH"
     gene            complement(8069..10573)
                     /locus_tag="VFC15_08730"
     CDS             complement(8069..10573)
                     /locus_tag="VFC15_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10280.1"
                     /translation="MGTILHEIRLGLRQMRKAPGFTIVAVLTLALGIGANTAVFTLLD
                     QALLRSLPVSHPEQLVRLHWTGDAPGHFNSYGGDDTDYFSYPMYRDLRDTNQVMQAVI
                     ANDQQNVAVRWNNKPDMASCELVSGNYFEGLGIRPALGRLFVPSDEALNGNLVAVLSF
                     NYWKTHFASDPTVINQTLFINTQPFTIVGVAPPGFHSIVAGSVEDVFVPVTAKGVITP
                     RWQDLEDRRSQWLTLSGRLKPGVSRQQAEAAMAPLWHSLRTEEFKTFNHKDRWRKRFL
                     DDSRLQLIDGARGFSPLRDQISTPLMVLMGMVGLLVLMACVNISSLLLVRAAGRAREI
                     SVRYALGAGRWQIVRQLLVEGVLLGLLGGLLGVVLAPAVSQLLIRRLLGGTSTELPFN
                     PNPDLRVLFFAFGLAFLVSLAFSLAPAVHFLKPDLVSALKQQTLTASGARLRLRRVSV
                     GVQIGLSLLLLIGAGLFVRTLRNLQSVDLGFTADHLLGFNVNPRLAGYQPDQVNALNR
                     RILESLAALPGIRAVAATDDPDLSHNDDSGSVKIVGYNAHEDEDMQAEQPWVTPQYFT
                     TMQVPLQAGRLFTDEDDVGKASVTIVNASFAQHYFGSPQASLGHVINFGTTEEPINST
                     IVGVVGDTKHQGVRAPVRRTVYRSLLQSTEPNFITYVVRTWQPPAAAESTLRTAMQQL
                     DSKLALSQLRTMDEMIADDLSSERLVALLSVSFGVLAMLLAAIGLYGVLAYSTVQRTR
                     EIGIRMAMGAQRISVVRMVLADVMWLAGVSIAVTLPLALLFARMIRNQLYGVSSFDPV
                     TLLAGTLLIVLVVVLSALLPARRAASVEPMKALRTE"
     gene            complement(10600..11082)
                     /locus_tag="VFC15_08735"
     CDS             complement(10600..11082)
                     /locus_tag="VFC15_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10281.1"
                     /translation="MHSIAHEVRYAIRQLAKSPGFAIVSVLTLAESVVLTVAGLVLGF
                     VVAYAVAPLLLRGLPPMLADHLQLSFRGPVLLFVVVIAVLCSLLCGVVPAWHRTQPGW
                     FNALSESGRSGTSGRVSMLHLVPWLAATAVLIATVLLASYLPARRAASIEPMKALRAE
                     "
     gene            11254..11598
                     /locus_tag="VFC15_08740"
     CDS             11254..11598
                     /locus_tag="VFC15_08740"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZM10282.1"
                     /translation="MSASGQEPDTGRLRYNEGTALRAEDLETKLMAQVKITVRRDGPF
                     RVEAPEGSVELVDADGTPYDLTGKPAFSLCRCGGSVNKPFCDGTHSKIGFQAAEAAVK
                     KIDEALPELPKS"
     gene            complement(11595..12836)
                     /locus_tag="VFC15_08745"
     CDS             complement(11595..12836)
                     /locus_tag="VFC15_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM10283.1"
                     /translation="MKFVKYKKYVPEPASEMSMEDLLAALSDHLLQSGFQRQYMDYYD
                     QNAAEQTLEDLRNAIAEALMNSDMLDQQLREQIESMSAEQFEQLIEQLIDRMEQEDYI
                     SIDQPHDPSRPSTVDGKAGESQAQTRFEVTDKGLDFLGFRALRDLLGSLGKSSFGRHD
                     TRDTATGIEASGASKIYEFGDTLNLDITATLSNAIQREGLHLPLNIEHSDLQVNQCEY
                     QSSCATVLMLDCSHSMILYGEDRFTPAKKVAMALSHLIRTQYPGDSLSLVLFHDSAEE
                     VPLSQLARVQVGPYYTNTREGLRMAQRILQRQRKDMKQIVMITDGKPSALTLEDGRIY
                     KNAFGLDPMVVSETLQEVSKCKRAGILINTFMLASDYGLVQFVQKVTEMCRGKAYFTT
                     PYTLGQYLLMDYMSRKTKTVH"
     gene            complement(12864..14369)
                     /locus_tag="VFC15_08750"
     CDS             complement(12864..14369)
                     /locus_tag="VFC15_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10284.1"
                     /translation="MSNLPQTLGELRRHPQFSEDHLRARRVKDELRDNLMVRLRAEGS
                     VFPGIVGYDDTVVPQIVNAILSRHNFILLGLRGQAKSRILRALTTLLDPQVPYLAGCE
                     IHDNPYAPLCPRCKKLVAEDPEGARIAYLTPDERYVEKLATPDVTIADLIGDIDPIKA
                     AKGGHELGSEMTVHYGLLPRANRGIFAVNELPDLAGKIQVGLFNIMQEGDVQIKGYPV
                     RLPLDVALVFTANPEDYTARGKIVTPLKDRIGSEIRTHYPATVEEGVAITDQEAWIER
                     NGHQLQIPDFIREVIEHTAFVAREDKRVDKRSGVSQRLPISALENAISNAERRAIRSG
                     EKLVVPRVGDIYAAMPSITGKLELEYEGELKGADSVARDIIRAAVVRTYDHYFHGNNN
                     MNQVVQWFDLGGEIQLRDSASAQEMLHKLRGIQGLLDKLSALGLSGKEPSEVVVSAAE
                     FILEGLYAHKRIGRSEERLFTAGEKQPRREKQNVREEDEERDFRRQRRPYN"
     gene            complement(14489..17095)
                     /locus_tag="VFC15_08755"
     CDS             complement(14489..17095)
                     /locus_tag="VFC15_08755"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZM10285.1"
                     /translation="MLLLIVAMGPSVGLAQQGSSGKVAPKASITAKPANPATNPLASV
                     LKGKPVVPIAPQELPAAIPEPAVPSVIPLPEVAARSQELSQTLRDLSSKLPSREQLES
                     IRTSLADLAPELQSKQKEVDALLKSTPNSLEVREQETYWRGMQTYTATWQDQLLTWAN
                     DAQAAIQKLDEQEPIWAATLRENQGSKDLDPVVALISDNLNDIRKLRAQAKDELQLIV
                     TLQIQASAGVDMAGNVLDRLAQARKQLTGHLIDRDSLPLWKVATRRQLGENSQNFGSI
                     NNRWIAIAAFFHENAGALFFVGVLLIVSQGLAYRLHLMVRELKPEQDLEPTVRRILHH
                     WFALGLLPPLMFGYLLVPTAPMSLIGIVILISFIPILLLLPPFLERRFRLMLYFLVGF
                     SAFNAAVNWIPLDPTLKRELHFLANAALFVVLAYVLQPFGVARAKGESLVRRLVLLGL
                     YIALAALGISIVANLFGYVRLAQFLAAACLYSAFIAISAFTALRVFTVLFLAGIATPA
                     AERIAAVRLHRPAITRWVPRLVSWAGIFIWLTATLDLLSLSESFTDAVSRFLDFRIAG
                     SASGITLGSVLGFFLIVLVGYFAAAAIRFGLREEILKRFQLSRGLPELISATLYYVVL
                     LMVFLSAVNVAGVELNKFTVLTGAIGVGVGFGLQNIINNFVSGLILQIERPIHIDDVL
                     EIDGTTGKVTRIGVRSSTVQTFQGAEVIIPNANFISSKVINWTLTESQRRLDLPVGVA
                     YGSNAKIVMRLLLEAATKHEYVLTKPEPVVLFKAFGDSSLDFELQFWVMQENNGVKIK
                     SEVALAVMQLLDDAGIEIPFPQRDLHLRSVDQNAAGLLQSNEPQKLDSTVENQLEALS
                     PKLGGIRRPSGD"
     gene            complement(17285..17611)
                     /locus_tag="VFC15_08760"
     CDS             complement(17285..17611)
                     /locus_tag="VFC15_08760"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10286.1"
                     /translation="MADESRVAALQEILRESPNDAFARYALGLEYSGAGETDAALAEF
                     AKLLEAHPDYTNGYFMSAQALARAERIDEARVMLLKGIESARRTNNRHALSEMEAMLD
                     ELEHGN"
     gene            17845..18351
                     /locus_tag="VFC15_08765"
     CDS             17845..18351
                     /locus_tag="VFC15_08765"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZM10287.1"
                     /translation="MESSETVLQPRPVSESKSEMIELVLPNDTNPLGALLGGRLMHWI
                     DLAGALAAHRHSHSYVVTASMDHIDFLVPVHVGDMVLFQSSVNRVFKTSMEVGVRVSV
                     ENYISSSRQKVAKAYLTFVSVDRHGRRLPVNPVIPETEDEERRYEGALRRREASKAER
                     DQRNAEKS"
     gene            18434..20008
                     /locus_tag="VFC15_08770"
     CDS             18434..20008
                     /locus_tag="VFC15_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10288.1"
                     /translation="MRYICATAICLMLLVCAIGLAAQQKEFPEPAKPSEKGAQTSEKS
                     GKLPADAERVAPKEEPPIVTHHEIHIGGRVLHYTATTGYMPIRNEEGTEVDANIFFVA
                     YTLDNPSPKRPLMFSFNGGPGSASIWLHLGAIGPRRVKMLPDGGMPQPPFELVDNEDT
                     WLDQADLVFIDPVGTGYSRATKKELAKKFFSVKGDISSVAEFIRLYLSRYQRWNAPLF
                     MVGESYGTTRAAGLSGYLIEHGIALNGVILVSSVLNFQTLEFTRGNDLPYILYLPSYA
                     STAWYHKKLSSDLQQRDLASVLTDAQQFAAGAYASGLGKGDSLSPQERQTLVDQVSRF
                     TGIDRSVVDENDMRLTQGVFCRELLRSQRLMVGRLDSRFTGDELTRVRGEGQQFDPSM
                     SVIRPPFTATFNNYVRSDLGYKTDLEYYVLGGGVTDWDWGSAGEGFPDTSEALRLAFV
                     KNPYMKLFVASGYYDLATPYFATQYTLSHMNLNTQQHAKITLGYYDAGHMMYIKSDAL
                     ARLKQEVSGFLNGALQ"
     gene            complement(20024..20809)
                     /locus_tag="VFC15_08775"
     CDS             complement(20024..20809)
                     /locus_tag="VFC15_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM10289.1"
                     /translation="MSDSNPVVSALAVPDPVVGQPGQRKLLSARLVRSVFLSDQTKHL
                     EFAVEGVNEFRFVPGQFVSIQQPKPDGKTHTRAYSLASTPRAGAQFDLCLNRVEQGFL
                     SNWLCDLEEGTAVEFHGPHGMFTLREPRHDAVFVATGTGIAPFRGIVHWLFEHPERNQ
                     GHEFWLVYGTRHEDGLYYREEFLAIEKAHPNFHYLPTLSRGGDEWQGLRGYVQDHVRE
                     IATGRSDMRAYICGLHQMVDANRALLKDELGWDRKQIVFERYD"
     gene            complement(20931..22799)
                     /gene="glmS"
                     /locus_tag="VFC15_08780"
     CDS             complement(20931..22799)
                     /gene="glmS"
                     /locus_tag="VFC15_08780"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZM10290.1"
                     /translation="MCGIVGYVGKKRVVPVIIDGLRRLEYRGYDSAGIAVAGNGEGLQ
                     VRRAEGKLRNLEEVIQLKPLDGTYGIGHTRWATHGRPTEENAHPHRDCTGKIVVVHNG
                     IVENYMSLKKKLIEEGHKFRTETDTEVIAHLIEKSLADADHKIPLEEAVRRTVKQLTG
                     VFAIAVISSDEPNKIVAGRNGPPAVIGLGDDEYFVASDVPAILYHTRDLFFLGDGDLA
                     VITADGVQLTDFDGHPITRQVQHITWDPIMAEKGGFKHFMLKEIFEQPRAVRDTTMGR
                     VSLDTGKVFLDEMDISESDLRKLQRVYIAACGTSWHAGLAAKFMIERLAKVTVEVDYA
                     SEFRYRDPLTGPEDLTILITQSGETADTIAAMRESRAKGSKTLSVCNVVGSMIARESN
                     GTIYTHAGPEIGVASTKAFTAQLTALFLFAVYLGEVRGTISPEEAKKLLTELTLIPGK
                     LEALLGSDDETEELAKRYHRAHDFLYLGRGIHYPIALEGALKMKEISYIHAEGYPAGE
                     MKHGPNALIDEDLPVVIMATRDLDDPGSVLRYEKTISNLKEVKARSGTVIAVATEGDE
                     EIKESADHVLYVPAAPELLVPILEVVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(22997..23242)
                     /gene="purS"
                     /locus_tag="VFC15_08785"
     CDS             complement(22997..23242)
                     /gene="purS"
                     /locus_tag="VFC15_08785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZM10291.1"
                     /translation="MKAYVYVSLKKSVLDPQGKTIHGALKKMGYRGVNDLRQGKYFEI
                     ALDDSIGREDAQAEVERIAREVLTNPVIEEYRFTLET"
     gene            complement(23301..23705)
                     /locus_tag="VFC15_08790"
     CDS             complement(23301..23705)
                     /locus_tag="VFC15_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM10292.1"
                     /translation="MATYGLVEYAEAGPEVRAVYDDIMATRKVDWINNFWKAIAHDPV
                     LLKRTWQSIKQIMAPGAIDPLVKEMIYVAVSVTNGCDYCIASHTASARKAGMTPAVFH
                     ELMSVVGMANETNRLVKGYQVEIDDAFKTPSS"
     gene            complement(23720..25225)
                     /locus_tag="VFC15_08795"
     CDS             complement(23720..25225)
                     /locus_tag="VFC15_08795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM10293.1"
                     /translation="MPTSRVESYKRGGSQSRDQRTKEASLSYIQFLGAAGVVTGSKHL
                     INTGDDSGKRGIQVLVDCGLFQGQKEWRERNWQDLPIPAREIDAVILTHAHLDHSGWI
                     PRLVKQGFHGPIYATPATIDLCGILLPDSGHLQEEDAAFHNRFKTSKHDPALPLYTME
                     EAQQCLSLFKAVKFNEPKRLSDEISFQFVRAAHILGSSMAEITLTSQGKTSRLLFTGD
                     IGRVRNSEISPGRVLYAGPTEHERCDYLVMESTYGNREHPHDDPRPELAALIRSTVQR
                     GGTVVIPAFAVERTQKLLFMLKELMETQQIPRVPIHADSPMAIKAVQIFLKYTDEFSP
                     ETKALIDKYGSPLDWANFYFDLTQDDSKKINQSTYPSIIVSSSGMATGGRVLHHLMQR
                     LPDPKNMILFIGFQAQGTRGYDIKNNATEVKIFGQMVPVRAQVAALEQFSDHADTPEL
                     LEWLRTFQAAPKQTDLVHGEPTAALALRTTMEKDLAWNVEVAAYKQKVQIV"
     gene            complement(25237..>26211)
                     /locus_tag="VFC15_08800"
     CDS             complement(25237..>26211)
                     /locus_tag="VFC15_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10294.1"
                     /translation="EHSGATLFHTPEKISLARDYAAAAEANESVLHEWLNPAVQVPTV
                     IELTDPNTSPYQNGAVLFTPLRQSETATLSLLLLPVQVAARFPSPHRWIEDGLQRFLQ
                     VVSVERRSRRKGALQFLDEYLAPLVKAEESASAEPGSAAKSTDSHAADDTLLNTSDEI
                     LLRGKGSFVFWMLRDMMGDEALQHALATYRPGADLNPAYFQNLLQEGKKRDLEWFFDD
                     WVYRNRGLPDFRVENAYVRPLLGDPSKIVLVTVTVENRGNAGAEVPVVIQTPSGERVV
                     RVLVRAHQKASGRSQVPAPPTKVVVNDGSVPEINSGDNTFEIPASPAP"
BASE COUNT         5560 a         7491 c         7713 g         5447 t
ORIGIN      
        1 acgtgacttt ttgcaggccc tcgacgccat cgttggcggt gtcggtttcg tagccccagg
       61 cctgaatcaa ctcggcaagc ccagtgcgct cattttcctc gtcttcgacg atcagtactt
      121 tttcttgatt cattccaaac agcagcctct cggaagtaac ggacccggct agtcacgtag
      181 caaataatac tcaccagcct ctttaatcgt gaaccgtgcg atccggcaac tccgcgttcg
      241 tttcgtgccc gacggtcctg cagggagatg ggcgaccaag cggcttccca taaaggtaac
      301 acttcttggc gccgggttgg ggccattagg ctttaccggg attcgggcgc tttcgcgatt
      361 gcccagcgta gctggactcc ttgcgcgaag cgaattcagg tcacgagaga accaattgtc
      421 tgcgggcttt gggatttccc tgggaaccat attcggaatc gcccccttgg cgctcaggga
      481 acggaatggc gtttgcgcga ggatcgttca ggggggggca ggcaaaatcg tggcaccgag
      541 aggaaattcg cgcccttcct caggaacgat ttttctgcaa aattcccctt acgaagccga
      601 tggccgatgt tgtacgataa aagaagctgc agctcagcct tacgtcgggg cgtgtgttcc
      661 tcgaaaaaga accgagggac gcgatggtga ggtgcaaatg atctggcgtg gacagtgcaa
      721 gagacccggt tataaagtca gtaaacatcg gaaaacagta gttttgtata tctcgtcggt
      781 tgctgtacaa tcgggagctg ttacatagca gttagatctt tattgtgcct gataagttca
      841 aaattgaacc cgagtgggcc actttgctga cggtactgct agagttccgg aggattgaga
      901 atggcgtttt gtgatggagt tccgaaacgg cttgtagcga cttcgatcgc gcttgcgttt
      961 gcggtttgct ttttgagttc atttgccgca gcgcaaatta ctttgcagcc gagagcggaa
     1021 gtgttcgggg gctattcgtg gtaccacccg aatggctacg ttgactgggg caaagtccct
     1081 gacatcgctc acggctggaa tgtgtccagc acattctatt tccccaacgc acgtaatttg
     1141 ggcgtggtaa ttgatggcag tggccactac aacggcacct attccaatag cggctttggg
     1201 ttggcaggat tgcagtataa gttccgcaac aatcaatttt caccattcgt gcgcgtgttg
     1261 gttggcgcca cgaagatcag ccccgcggcc ctcaactcag agtggcgcgc gacagttggg
     1321 ggcggcggcg gtttcgacct gaccctcagc aatttgatct ccctgcgact ggcgcaggcg
     1381 gattatctat acaccagcta caatacccag accttcattg accacaattc aacgtggaac
     1441 agcgttcgcc tgtcggctgg tgtggtgttc aacctgggca attactacac tgccccgctg
     1501 gcagcggctt gctcggcgag cccgacaaca cctgtttttg ccggcgaacc ggtaacggtc
     1561 accacgaccg gaacgaactt cagtcccaag cacactttgg cgtatgcctg gaccgccacg
     1621 ggtggcaaac ttgctgcccc gagtgcggca agctcggccg tcgatacgac gggcctggcg
     1681 gctggcacct acaccgcgaa cgcgactatc accgatccga agtggaagaa gaacaactcg
     1741 gcgagctgct cgacgagttt cacgatcaaa ccgttcactc cgccttcggt aacgtgctcg
     1801 gcgagtgtga caactgtgaa gtcgggtgag acagccacca tcacggcgaa tgcaaccagc
     1861 caggatggcg cccagatcag caactatgct tatacaactt cggctggcaa gatcactggc
     1921 aacgggacct cggcgactct cgacacgacg ggtctgcctg gttcgacggt cagtgtgacg
     1981 gtcaccgcaa ccgattcgca tggtctgtcc gctagctgca ccgcttcggt gggtgtggtt
     2041 gaggaaatta agtgcgtcag catcgaggat tggggcgagt gtaccttcga gaagaacccc
     2101 aagaaaccgt ggcgtgttga caacgattgc aaggacacgc tcgacaaact gtctctccgt
     2161 ctgcaacaga tgccctccgg taaggtggaa atcgttggtt acacggacga gaaggaatct
     2221 gtcgacgtta agcaactcgg tgctcagcgc tccgtcaacg tgaagtacta cctgactacg
     2281 gatggcccaa ccaagtcgga cgccacccgt atcgagcctc gcgatggtgg gacaaagggt
     2341 aaggcgactc acttcttcta tctgcccgac ggagccctgt gctctggcca ggcggtcgaa
     2401 ggaacgccgg ttgacgaaac ccaagtgcag ccgcagtcgc gtaacgcgcc tgctcctgcg
     2461 aagaagcaca aggcgaaagc caagcctgcg gcgcccccag cgcaatagct gaagtgccgt
     2521 agttcgactc aagccggtga gagggaaccc tctcaccggc tttttcctga agcacgaaag
     2581 aactggagct ttcggaaatg gggatcgtgg attcccggta attcccagcg gaccgagatt
     2641 gccgggcatt ggttggccac attgcgcgga ccatctttct caagcgagtt gtgtccgcgg
     2701 ttggactttc catatcccac ccctgcgagc cggcaggcat gtgatttggc actgtgcgcc
     2761 gtgccctgtg agcgcataag atttagatgt ggtgtcttca gtcaataagt tacgaagggg
     2821 agatgtagtc gtgggtatgc ggaatctggt cggttcagtc gcgaagctgg ttgttggctt
     2881 tgcgttgctt tttacaattt gcaccgtaac tgcatgggct tcggattggc agttgatcgg
     2941 tcccgatggt ggcaacgtgc gctgcctggc ctacgatccc agcgatccga accggattct
     3001 tcttggaacc agcgcggggc agttgtttag ttcgcgcgat ggcgggaatt cctgggtccc
     3061 gtttgcgcat ctgggttccg gagatgacct ggtaatcgat cacatcgttt tcgatccgac
     3121 caacccccag accatctatg cggcagcatg gggcctgtac cacgacgacg aaggagatgt
     3181 cttccgctcc gatgacggtg gggccacctg gcgcacgctg gcgggagtac acggcaaatc
     3241 catccgcgcc ctggcgatgg cgccgagcga tcacaacacc ctggtgatcg gggcactgga
     3301 tggagtgttc cgttcgcaga atggtggggc gacttgggag aagatgacgc ctgagaaccc
     3361 tgacgtcctc gagaaccacg cttcgatgag gaatttcgta tcggtggcgg tggatcccca
     3421 gaatccagac gtggtttacg ctggcacccg ccacttgcca tggaagacct ccgatggcgg
     3481 aaagagttgg cataacttaa aagacggcat cctggacgat tcggacgtat tcagtattat
     3541 cgtcgatccc aaaacaccgt cgcgggtgta cgcaagcgcc tgctcgggga tttacaagag
     3601 cgacaatggt gccgagttat ttcatcgcgt ccagggactg ccccattccg cgatccgcac
     3661 cagggtgctg aagcaggatc cgcaacggtc ctccatcgtt tacgctggca ccacaggcgg
     3721 gatgtggaag acagtggatg gcggcaccaa gtggacgctg gtgacgtccg ccgacgtagt
     3781 gatcaatgac gtcttgatcg atcctcggaa tccgcagcgc gtactcatcg ccacggaccg
     3841 gggaggagtt ctggcaagca acgatggctt cgccaagtac cagtcgtcaa accggggatt
     3901 ttcgcaccgc atggtcggat cggtgatagc cgaccgcaac aactcgaacc gcctctatgt
     3961 gggtatcgtc aatgacaaga ccgacggtgg attattcatt accgacgatg cgggaaaaag
     4021 ctggcagcag gtcagcaagg gattgggcga ccgcgatgtg ctaagcctgc aacaagcgga
     4081 gaatggcgcc atcttcgccg gcacaaacca tggcatattc tcgctcgcca cgttgagtgg
     4141 tgcgtggctg ccggccacca tgattcatgg cccggtcccc gaatggcaac ccatggcgga
     4201 agcagttcca gaagcggcag ctcagaagac gctgaagtca aagacggcga agggcaccgc
     4261 caaaaagccg gcagtgacgg cgaagaagaa agagccagcc gaggtgccga tcccaatcgc
     4321 aatcgcaccg cgggtgcgct cgatccagat caccaacaaa gcatggtatg ccgctaccaa
     4381 cgatggattg ttcatcagcg tggatggcgg caagaaatgg tatgggactc cgatcgatgg
     4441 tgaaagcgac ttcctggcag cgaacgacta tcctgacggg acccttaccc tggccagcat
     4501 taagcgtgcg ttcatttctc gggacgcggg acgaacctgg tccgagattg tggctccgac
     4561 ctacgtgact ggtctctaca acgtcaccat gactcaggat tcgtcgcttt ggctgggagc
     4621 cagggagggc gccatacgtt ccaatgacgg cggcaagagc tggtctcatg tcatgaacgg
     4681 actgccaccg agttatgttc ttggagtcaa gtacgacgcc aaagctcagc ggttgcttgc
     4741 cacagccatg aactcgcgcg gggtctttga aagcgtggat ggcggcaaga cgtggcatca
     4801 gacccccgag gccagctttt ctattcggac agccatgggt tatcaggacc tgctgctggc
     4861 gatttcctgg cacaacggac ttctactgca gcgcggcgac agtacggctt cggtatcgaa
     4921 tgccgcggtt actgtgggca gtgcagcggt caagagccag cagtaaagca gcgtgggcgc
     4981 gataacccaa ccgggaggca accagcctcc cggtttttgc agcttagaaa agcagcggag
     5041 tgagtcgatt tcgtgttgag cacatcccgc cggggccctc acttcgctcc cgatctggga
     5101 atcgagcgca ttttgctccc attcccgaaa tcggaattca agacgtgcat ttcagggtag
     5161 ttacaagcat ctgtcgaaac gtgctctccc gttttgaaac tcatttcgaa tcggcgctga
     5221 ggcagagtgt gacggatttt cttgccgttt gttgataaca catggcttgt cgatcgcgtt
     5281 tggtttggca tcgaccgtgc tttagtagtg tgcgtacgag cggaaaatct gaagcgaggg
     5341 acgggtcatg aatgcatttc gaagtgcgga ttgcggcgaa aaggtcggga cgaatccggt
     5401 agctgccatt gattgcaacg tggacctgtg tgggcgcact cgcgctggaa ttcccgagga
     5461 tggctgcaac gattgcggcg cgatacttca tggattgaac aacgctttgg ctgcgatgct
     5521 gctaaatgcg caggtattgg aatggaagct accctcctac agccgatcca gacgctatct
     5581 ccatgaaatt gagcgaaatg cgcaacgcgg tggggccttg gtgaagcaac tgctgggccg
     5641 tatggaagca aggtgcgaga ttggtctgag cggttgtcga attgacggag aggggctgac
     5701 cttggctggc gacgatggcg tcgcgtcggc tcgggaactc gccactgtgg gccaagtgga
     5761 ggagccaccg ctgggacaaa ccgcgcgtcc cgccggtgtt ttgctggcac atcggaaaaa
     5821 ggtacctcac acgatggtgt gatccctgca ctagcatcaa tttcccaaaa agggacaatg
     5881 gttatgcaga cggcgccaca acctcaatcg cgactgcttt ggaaatccaa gccgtttgga
     5941 agaaggcctg accgtgctta cagtagagcc agtttcagca gtggaacaga ccgccgatac
     6001 gaacctcctc aatctcctca tcgtggacga cgagcgctca gtgcgtgagt cctgtcgaga
     6061 agtggccgtg gcgctgggct ttaacaccca gattgctgac tctccggagc actgctacca
     6121 cattctggat acgacgaacg tggacgtcgt gttactggat cttcggctgc cggggacgaa
     6181 tggcgtggaa gtgctccgcg aaattaagcg gcaccggcca gagacggtgg tcgtgatcat
     6241 gaccggattt gccacggtcc agtcggcagt acaggccatg aaggccggcg cctatgacta
     6301 tgtcaccaag cctttcagct tcgacgaact ccggctggtg ctggatcgcg tgaccgcgca
     6361 tgtgaggctg acgacggaga atcggctgct gcgcgagcag gtgaaatcga agcatgggtt
     6421 tggagcgatg gtcgggcagg caccggagat ggacaagctg taccgcatca ttgccaaggc
     6481 cacacacagc acccatccgg tgcttattct gggcgagagc gggactggca aggaattggt
     6541 ggcccgttcg atccattatt cgggtccatt ccgtgacaag ccgttcatcc ccgtcgattg
     6601 cggttccctg gtcgcgacac tgatcgagag cgagctgttc ggatacgtga gaggcgcgtt
     6661 taccggtgcg gtgcgggcaa aagatggtct tatggccgca gcagagggcg gaacgatatt
     6721 tctcgacgaa atcggcgagc tgccgctgga tttgcaatcg aagctgctgc gggcgttgca
     6781 ggagaaagaa attcggccag tcggcggcac gcgggcgatt cccatcaacg tgcgaattct
     6841 ggccgccacc aaccgcgacc tggaagcggc ggtgcaggat ggcacgttcc gccgcgattt
     6901 gtacttccgg ctcaatgtac tcacgctcag ggtgccgccg ctgcgcgagc gcaaacagga
     6961 cataccactt ctggcgggac acatcctgga acgcgtggcc cgcaccacag gggtgcagcg
     7021 caacataagc gacgacgccc tcaaactgat gctcaactac gaatggcccg ggaacgtgcg
     7081 cgaattggag aactgcctgg aacgcgcgtg cgcactgact tcgggtccca ccatgcacat
     7141 tgctgatctg cccacggcgc tgcagaactt tcacacccag acgccgatgg tgccggtcag
     7201 cctcgagtcg accggcattg cgcctctggc ggatttggag aagcgggcga ttctgcacgc
     7261 tatcaccatt ctgaatggag acaagctgga ggcggcccgc cagcttggaa ttggcaagac
     7321 gaccctctat cgcaaactga aggaatacgg ctcagtggtg taatccacgg cgcgcattct
     7381 tttcccaatc aacctgcatt cacgcgggac cggtgaagtg tgtccggatc cgatgacggg
     7441 agaggtttca tggttatcct ggtaacgacc tcgccgcgcg ccaaagaatg cgctacggcg
     7501 attgaacaga cgacacatca aaaaacccag atcgcaacgt cgctggcgag ggctgtcgag
     7561 tgcctgcaga cccacgacta cgatgccctg gtactcgacg agtcgttcaa ccatgcggag
     7621 attggcgggg ccaatttgtt gctgaaccat gcaggttcgg cgatgccgat ttacgttaat
     7681 ctggcactgc acggcacgga gagggtaacc cgcgaggtgc aaaacggcat gctgcgattt
     7741 gtgcgggaga agctggccgc catgcggtcg gcggagaacg tgctgcgaag tttgttgcga
     7801 ggcgaagtca cagccatcct gctgaattcc gagttggcct tgcgcgagcc atcactctcc
     7861 gcaagcatcg cagagaagat tcatattatg cacgatctgg ccgaggggat gcgtctgaag
     7921 ctggaaggcg cgccggccga tgggatgggg gaacacacac atatccccgc cgccatcgct
     7981 cacgaaccca ggccccgacc ccactagact acaaagcgac catggctctg acgttggcca
     8041 atcagttggc agcgacggac agcaacactc actccgtgcg cagtgctttc ataggctcaa
     8101 ccgaggcggc ccgtctcgca ggcagcagag cggagagcac caccacgagc acaattagaa
     8161 gcgtgccagc cagcagggta accggatcga acgaactcac tccataaagc tggttgcgaa
     8221 tcatgcgagc gaacaacagc gcgagcggta gcgtgaccgc aatgctaact cccgccagcc
     8281 acataacgtc tgcaagcacc atgcgaacga cgctgatgcg ctgagctccc attgccatgc
     8341 gtataccgat ctcgcgcgtc cgctggacgg tggagtatgc cagcacgccg taaaggccga
     8401 tggcggccag cagcatcgcc aatactccga agctgacgga caacagggca accagccgtt
     8461 cactggagag gtcgtcggcg atcatctcgt ccatggtgcg tagttggctt agcgccagct
     8521 ttgagtccaa ctgctgcatg gcggtgcgta gagtactctc ggccgctgcg ggaggctgcc
     8581 aggtgcgcac gacgtaagtg atgaagttag gctctgtcga ttgcaagagc gaacggtaca
     8641 ccgtgcggcg aaccggggcg cgtactccct ggtgtttcgt gtcgcccacc acgcccacga
     8701 tcgtactgtt gattggttcc tctgtggttc cgaagttgat cacatgcccg agggaagctt
     8761 gcgggctgcc aaaatagtgc tgcgcgaagc tggcattgac gatggtaaca ctcgccttgc
     8821 cgacgtcgtc ttcgtccgta aacaggcgtc cggcctgcag tggcacctgc atagtggtga
     8881 agtattgcgg cgtcacccag ggctgctccg cctgcatgtc ctcatcctcg tgtgcgttgt
     8941 agcccacgat tttgacactg ccgctgtcgt cgttgtgaga aagatcagga tcgtcggtgg
     9001 cggcgacggc acgaatgcca ggcaatgcgg ccagtgattc aagaattcgc cggttcaagg
     9061 cattgacctg gtcaggctgg taccccgcca gacgaggatt gacgttaaat ccgagcaggt
     9121 ggtcggcggt aaatcccaag tctacggatt gcaggttgcg cagagttcgc acgaaaagcc
     9181 ctgctccgat cagcaacagc aggctgagtc cgatctgtac acccaccgaa acccgtcgca
     9241 ggcgcagccg cgcaccggac gcagtcagag tctgctgctt gagcgcactg accagatcag
     9301 gcttcaggaa atgcaccgcc ggagcgaggc tgaaagccag agaaaccagg aacgcaagcc
     9361 cgaaagcgaa gaacaatacc cgaagatcgg ggttcggatt gaacggaagt tccgtgctgg
     9421 tgcctcccag caacctgcga atcaggagct gcgacaccgc gggggcaaga acgacgccaa
     9481 gcaaaccacc gagcaatccg agcagaacac cttcgaccag caactggcga acgatttgcc
     9541 agcggccagc acccagcgcg tagcgcactg agatctctcg cgcccggccg gcagcgcgca
     9601 ccaacagcag gctggagata ttgacgcacg ccatcaacac cagcaggccg accatgccca
     9661 tcagcaccat gagcggtgtg ctgatctggt cgcgcaaagg tgaaaagccg cgggccccgt
     9721 cgatcaactg caatcgcgag tcgtcaagaa acctttttcg ccagcggtcc ttatggttaa
     9781 aggtcttgaa ttcctcggtc cgcaacgaat gccagagcgg cgccatcgcc gcttcggcct
     9841 gctgtcggct gaccccgggc ttcaggcgtc ccgacaacgt cagccattgc gaccgacggt
     9901 cctccaggtc ttgccatcgt ggcgtgatga ctcccttcgc cgtgaccggg acaaatacgt
     9961 cttctaccga ccctgcgacg atgctgtgga agccgggcgg agcgacaccc acgatggtga
    10021 atggctgggt gttaatgaag agcgtctggt tgatcaccgt gggatcggaa gcgaagtgcg
    10081 tcttccaata gttgaagctg agcaccgcca cgagattccc attcaacgcc tcgtcggacg
    10141 gcacgaacaa acggccgagc gcgggacgga tccccagccc ttcgaaataa ttgccggaga
    10201 cgagttcgca gctggccatg tccggtttat tattccagcg aacggcaaca ttttgctggt
    10261 cgtttgcgat taccgcctgc atgacttgat ttgtgtcgcg caggtcgcgg tacatcggat
    10321 acgaaaaata atcggtatca tcgccgccgt acgagttgaa gtggccgggt gcgtcaccgg
    10381 tccaatgcag acggacgagt tgttcgggat ggctcacagg caacgaacgc aagagggcct
    10441 gatcgagaag cgtgaagact gcggtgttag cgcctatgcc caacgccagt gtgagcaccg
    10501 cgacaatcgt gaatcctggc gccttgcgca tctgccgcaa gcccagtcga atttcatgca
    10561 ggatcgttcc cattatttcc tcttctcgtg accgttcagt tactcggctc gaagcgcttt
    10621 catcggttca atggatgccg cgcgacgtgc tgggagataa cttgccagca acaccgtggc
    10681 gatcaataca gcggtagcgg ccaaccaagg gaccaggtgc agcatgctca ctcgtccaga
    10741 ggttccggac cgtcccgatt ccgacaatgc attgaaccat ccgggttgcg tgcggtgcca
    10801 ggcgggaacc acgccgcaca acagagagca cagcacggca atcacgacga cgaacaacag
    10861 tacgggcccc ctgaacgaga gctgcaggtg atcagccagc ataggtggca ggccgcgcag
    10921 caacaggggc gcgacggcat aggccacgac gaatcccagc accaggcctg cgacggtcag
    10981 gaccacgctt tccgcgagcg tgagcaccga tacgatcgcg aatcccggcg acttggcgag
    11041 ctgtcgaatt gcgtaacgaa cttcgtgtgc gatcgagtgc acgtcgagtc tcctccaact
    11101 tccgcgaatc accgtctcga atcttgcccg gtgcgatgag cacgagagca atatccgaga
    11161 acgataggtg caccccggca gccactctga ggggcaaatt cttcagtgac ttacaagctg
    11221 ttgaatcaag actctgtccg cagatgttac ccaatgtccg cttccggaca ggaacctgac
    11281 accggcagac tccgctacaa tgaagggacg gcgctgcgcg ccgaggattt ggagaccaag
    11341 ctaatggcgc aagttaagat tacggttcgt agagatggcc cgttccgcgt ggaagctccc
    11401 gaaggttcgg ttgagctcgt tgatgccgat ggcaccccgt atgacctgac tgggaagccc
    11461 gcattttcgc tgtgtcgctg cggcggatcg gtgaataagc cgttttgcga cggaacccac
    11521 tcgaagattg gattccaagc ggccgaggcc gcggtgaaga agatcgacga ggcgctgccc
    11581 gagctaccta agagttagtg cacggttttc gtcttgcgtg acatgtagtc catcagcaag
    11641 tactgaccaa gcgtgtaggg cgtcgtgaag taggccttgc cgcggcacat ttccgtgacc
    11701 ttctggacga actgcaccag tccgtaatca gatgccagca taaacgtgtt gatcaggatc
    11761 cccgcacgct tgcacttcga gacttcctgc agtgtctcgc taaccaccat gggatcgagg
    11821 ccgaaggcgt tcttgtagat gcgcccatcc tcgagagtaa gcgccgaagg tttgccatcg
    11881 gtgatcatga cgatctgttt catgtccttg cgctggcgct gcaggatgcg ctgcgccatg
    11941 cggagtccct cgcgagtatt ggtgtaataa ggccctacct ggacccgcgc taattgcgac
    12001 aacggcactt cctcggcgga atcgtggaac agaaccagcg aaagcgaatc ccccggatac
    12061 tgtgtgcgga tcaggtgcga cagtgccatc gcaactttct tggcgggcgt aaaccggtct
    12121 tcgccgtaca aaatcatgga gtgggagcag tccagcatca gcacggtcgc acacgacgac
    12181 tgatattcgc actgattaac ttgcaagtcg ctgtgctcga tgttcagcgg aagatgcagc
    12241 ccttcacgct ggatggcatt cgacagcgtc gcggtgatgt cgaggttgag cgtgtcaccg
    12301 aattcgtaaa tcttcgaagc cccgcttgcc tcgattcccg tagcggtgtc gcgggtgtcg
    12361 tggcgtccaa agctggactt gcccagcgag cccaacaagt cgcgcagtgc gcggaatccc
    12421 aaaaagtcca gccctttgtc ggtgacttca aagcgggtct gagcctggga ttcgccagcc
    12481 ttcccatcaa ccgtagacgg acgcgaagga tcgtgcggct gatcgatgct gatgtagtct
    12541 tcctgctcca tgcgatcgat gagctgctcg atgagttgct cgaactgctc ggcgctcatc
    12601 gactcgattt gctcgcgcag ttgctgatcg agcatgtcgc tgttcatcag ggcttcggcg
    12661 atggcgttgc gaagatcttc cagcgtctgt tcggcggcgt tctggtcgta gtaatccatg
    12721 tactgccgct gaaacccgct ctgcagcaga tggtcggaga gggccgcgag caggtcttcc
    12781 atgctcatct ccgacgcggg ttcgggaacg tatttcttgt acttaacgaa tttcatggga
    12841 attgcagttc gcgattccgt tctttagttg tacggcctgc gttgccggcg gaaatcccgt
    12901 tcctcgtctt cctcgcgcac attctgcttt tcgcgacgcg gctgcttctc gcccgcggta
    12961 aacagccgct cctcgctgcg gccaatgcgc ttgtgcgcgt acagtccctc gaggatgaac
    13021 tcagccgccg ataccaccac ttcggacggt tccttgccac tgagtcccag ggcgctgagc
    13081 ttgtcgagta gcccctgaat tccgcgcagc ttgtgcagca tttcttgtgc gctggcgctg
    13141 tcgcgcaact ggatctcgcc acccagatcg aaccactgca ccacctggtt catgttgttg
    13201 ttgccgtgga agtagtgatc gtaggtgcgg acaaccgcag cgcgaatgat atctcgtgct
    13261 acgctgtcgg cccccttcaa ctcaccttca tactccagtt cgagcttgcc ggtgatggaa
    13321 ggcatagcgg cataaatgtc acccacacga ggcaccacca gcttttcgcc cgagcgaatg
    13381 gcgcgccgct cagcattcga aatcgcattc tccagggcgc tgatgggcag acgctggctc
    13441 actccggaac gcttatccac acgtttgtct tcacgcgcga cgaacgcagt gtgctcgata
    13501 acctcgcgaa tgaaatcggg aatctgcaat tgatgtccgt tgcgctcgat ccaggcttcc
    13561 tggtcggtga tcgcaacgcc ttcctccacg gtcgcagggt agtgggttcg aatctcggag
    13621 ccgatgcggt ccttcagcgg agtcacgatc ttgccgcgcg cggtgtagtc ttccgggttg
    13681 gcggtgaata ccagggcaac atcgagtggc aatcgcactg gatagccctt aatctgcaca
    13741 tcgccttcct gcatgatgtt gaagagtccg acttggattt ttccagcgag gtcaggcagt
    13801 tcgttcaccg cgaaaatccc gcggttagca cgcggaagca gtccgtagtg cacggtcatt
    13861 tcactgccca gttcgtgacc acccttggca gccttaatcg gatcgatgtc tccaatgaga
    13921 tcggcgatgg tcacgtcagg cgtggcaagc ttctcgacgt agcgctcgtc cggcgtgagg
    13981 taggcgatgc gcgctccttc aggatcctcc gcgaccaact tcttgcaccg aggacagaga
    14041 ggcgcgtacg ggttgtcgtg gatttcgcac ccggcaagat acggaacctg cggatcaaga
    14101 agagtggtca gcgcccgcaa gatacggctc ttggcctgtc cacgcaatcc cagcagtatg
    14161 aaattgtggc gcgaaagaat cgcattcacg atctgcggaa ccaccgtatc gtcgtagcca
    14221 acgatgcccg gaaacacgga gccctccgcc cgcaggcgga ccatgagatt gtcgcgcagt
    14281 tcgtctttga cgcgacgagc ccgcaagtgg tcttcggaaa attgaggatg acgacgaagt
    14341 tcaccaagag tctgaggaag attgctcaaa gctgggatac ctccagggcc acgaccgcca
    14401 acccacttgg cggaacgatt cttcgtattc tacgccagcg gcggagaaga cagttcacgc
    14461 gcaaaatcga gagatggtgg atggctcttc agtctccact cggtcgtcga atgcctccca
    14521 gcttcggcga gagcgcttcg agttgatttt cgacggtcga atccaacttt tgcggttcgt
    14581 tagattgcag caggccagcc gcgttctggt cgactgagcg caggtgcagg tcgcgctgcg
    14641 ggaacggaat ttcgatgcct gcatcgtcca atagctgcat aacggccagc gccacttcgc
    14701 tcttgatctt gactccgttg ttctcctgca tcacccagaa ctgtaattca aaatccagcg
    14761 aactgtcgcc aaaggctttg aagaggacca ccggctccgg cttggtcagg acatactcat
    14821 gtttcgtggc ggcttccaag aggagtcgca ttacgatttt cgcattcgag ccgtaggcaa
    14881 ctccgaccgg aagatccagg cgtcgctggg actcggtaag cgtccaattg atgaccttgc
    14941 tggaaatgaa gttagcgttg gggatgatga cctctgctcc ctgaaacgtc tgaacggtgc
    15001 tggaccggac gccaatccgc gtgacttttc ccgtggtgcc gtcgatctca agaacgtcgt
    15061 cgatatggat tggcctctca atctgcagga tcaggccaga aacgaaattg ttgatgatgt
    15121 tctgcaggcc aaagccaacg ccaacgccaa tcgcgccggt gaggacagtg aatttattca
    15181 gttccacgcc agccacgttc acggcggaca ggaacaccat caacagaacg acgtagtaga
    15241 gcgtggctga gatcagctcc ggcaggccgc gggacaactg aaatcgtttg aggatttcct
    15301 ctcgaagccc gaaacgaatt gcggctgcgg cgaaatagcc taccagcacg atcagaaaga
    15361 aaccgagaac gctgccgagc gtgattcccg atgcgcttcc cgcaatgcga aagtcgagga
    15421 aacggctgac cgcgtcggtg aaggattcgc tgagactgag caaatcaagc gtcgccgtca
    15481 gccagatgaa gattccagcc catgacacca ggcgaggcac ccatcgagtg atcgccgggc
    15541 gatgcaggcg gaccgctgcg attcgctcgg cagcgggagt agcgatgccc gccaggaaca
    15601 gcaccgtgaa aactcgcaac gcggtgaacg cgctgatggc gatgaacgca ctgtaaaggc
    15661 atgccgctgc cagaaactgg gccagcctca cgtaaccaaa aaggttggcg acaatcgata
    15721 ttcccagcgc cgccaacgcg atatacaaac ccagaagcac cagcctgcgt accaggcttt
    15781 cgcctttcgc gcgagcgaca ccgaaaggct gcaggacgta cgcgaggaca acgaacaacg
    15841 ctgcgttcgc taggaaatgc agctcccgct tgagcgttgg atccagagga atccaattga
    15901 cagccgcatt aaaagcactg aatccgacca ggaagtaaag cattaaccgg aatcggcgtt
    15961 ccagaaaggg cggcagcagg agcagaatcg ggataaagga aatcaggatc acgatcccaa
    16021 tcagcgacat tggagcagtt gggaccagca gatagccaaa catcagggga ggaagcaagc
    16081 cgagcgcaaa ccagtgatga aggatgcgac gtacggttgg ctccagatcc tgctcaggct
    16141 tgagttctcg caccatgagg tgcagacggt aggcgaggcc ctgggagacg atcagcagga
    16201 ctccaacgaa gaacaaagcg ccagcgtttt cgtggaaaaa tgccgcaatc gcgatccagc
    16261 ggttgttgat cgagccgaag ttctgagaat tttccccaag ctgtcgcctg gtagcgacct
    16321 tccacagtgg caagctgtcg cgatcgataa ggtgtccggt gagttgcttt cgcgcttgcg
    16381 ccaggcgatc gagtacattg ccagccatgt cgacaccggc gctagcttga atctgcaagg
    16441 tcacgatcaa ttgcagctcg tcttttgcct gggcgcgcag tttgcggatg tcgtttagat
    16501 tgtcgctgat caatgcgact acgggatcga gatcctttga cccctggttc tcacgcagcg
    16561 tggcggccca gatgggttcc tgctcgtcga gcttttgaat cgccgcctgc gcatcgttgg
    16621 cccacgtcag caactggtcc tgccaggtgg cggtataggt ctgcatgccc cgccagtacg
    16681 tctcttgctc cctgacctca agcgaattcg gagtggactt cagtagggcg tccacctcct
    16741 tctgcttcga ttgcagttcc ggcgccagat ctgcgagaga cgtgcgtatg gattccaact
    16801 gctcacgcga gggcaacttg ctggatagat cgcgcagcgt ctggctcagt tcctgcgaac
    16861 gtgctgcaac ctcaggaagt gggataacgg acggaaccgc tggctcggga attgctgcgg
    16921 gcagttcctg aggtgcgatg ggaaccaccg gctttccctt caagaccgat gcgagaggat
    16981 tcgtcgcggg gttggccggc ttcgcggtaa tggaggcttt tggtgctacc ttcccgctgg
    17041 aaccttgctg cgcgagaccg accgacggtc ccatcgcaac aatcagcaac agcattcgca
    17101 gcaaatgcga gaacgaggaa cttcctgtat aaaccggaag ggtcccttcg gaattcgcga
    17161 aactggccgg actttctaac ctgagcatgc tggcgtaatt atcgcctatg agcgcgcctg
    17221 gaccagcgca aatcttcatc gaagctgatg gtggcttatg gccccgcggc accgtgttgc
    17281 ggattcagtt tccatgctcc agttcatcga gcatggcctc catctccgag agggcgtggc
    17341 gattgttggt gcgccttgcc gattcgatgc ccttcagcaa catgacgcgg gcttcatcga
    17401 tgcgctcggc gcgagccagc gcctgggccg acatgaagta tccattggtg tagtcaggat
    17461 gggcttcgag caacttcgcg aactctgcca gcgcagcatc cgtttcgccc gcgccggaat
    17521 attccagccc cagcgcatag cgggcaaaag cgtcattcgg gctttctctt aggatttcct
    17581 gcagtgcggc tacgcggctc tcatcggcca tcgatcgctt ctcctgggtt tcgaaccaac
    17641 ggggttcgaa cgaatggtac aggagtgcgt gcgccaatct caacattcgg ctcctccgct
    17701 gcgccagcgc ggtgcggccg gtagcgcgtg accattacaa ttacacaaca gccttcactc
    17761 tgttgagtga gaagttgaag cctgttggtt gtcgaaatca agccactaag ccggatcacc
    17821 gagatcaggc caggaaggga tgacatggaa tcgagcgaaa cagttctgca gccgaggccg
    17881 gtgagtgagt cgaagtccga gatgatcgaa ctggtgttgc cgaatgacac caacccgctg
    17941 ggcgcgttgc tgggcggccg cctgatgcat tggatcgatt tggcgggcgc tcttgccgcc
    18001 catcgccatt cgcattctta cgttgtcact gcctcgatgg atcacattga ctttctggtg
    18061 ccagtgcacg ttggcgatat ggttttgttc cagtcgtcgg tgaaccgggt attcaagacc
    18121 tcgatggagg tgggggtcag ggtttcggtc gagaactaca tctccagttc ccgccagaag
    18181 gtggcgaagg catacctcac atttgtctcc gtggaccgtc atggccgccg tttgccggtg
    18241 aacccagtca ttccggaaac ggaagacgag gaacgcagat acgaaggcgc actgcggcgc
    18301 cgtgaagcct caaaggccga gcgtgatcag cgcaatgcag agaagtccta gtccggcatt
    18361 tcccatgata gagttgcacg gatcgcccgt ccgctgaagg gtcgcaattt acttgctatc
    18421 gatggagaac tggatgcgct acatttgcgc cacggctatt tgtcttatgc tgctcgtttg
    18481 cgccattgga cttgctgccc agcagaaaga gttccccgaa ccggcaaagc cgtctgagaa
    18541 gggtgctcag actagcgaga aatccggcaa acttccagcc gatgcggaga gggtcgcgcc
    18601 aaaagaagag ccgccgattg tcacgcacca tgaaatccac atcggtggcc gggtgctgca
    18661 ttacactgcg acgactggct acatgccgat tcgcaacgaa gaaggcacgg aagtcgatgc
    18721 caacatattc ttcgttgcct acacgctgga taatccctca cccaagcgtc cgctgatgtt
    18781 ttcgttcaac ggcggtcccg gatcggcgtc gatctggctg catctcggag ccatcggtcc
    18841 gcgccgcgtg aagatgctgc ccgacggcgg catgccgcaa cctcccttcg aactggtgga
    18901 caacgaggac acatggctcg accaggcgga cctcgttttt atcgatcccg taggaaccgg
    18961 gtacagccgg gcaaccaaga aggagctagc taagaagttc ttcagcgtga agggcgatat
    19021 ctcgtcggtt gcggagttca ttcgcctcta tctcagccgc tatcagcgtt ggaatgcgcc
    19081 gctcttcatg gtgggtgaaa gttatggcac cacgcgcgcg gcgggcctca gcggatacct
    19141 tatcgagcac gggattgccc tcaacggcgt catcctcgta tcgtcggtgc tgaatttcca
    19201 gacgttggag ttcacccgcg gcaacgactt gccctacatt ctctatctgc ccagctacgc
    19261 ctcgaccgcc tggtatcaca aaaagctgtc gtcggatttg cagcagcgcg atctcgccag
    19321 cgtactgacc gacgcgcaac agtttgccgc cggagcctac gcaagcggac tgggaaaggg
    19381 cgacagcctg tcgccacaag agcgccagac tctggttgac caagtgtcgc gattcacggg
    19441 gatcgatcgc agcgtcgtgg acgagaacga catgcgcctg acgcagggcg tattctgccg
    19501 ggaattgctg cgcagccagc gtctcatggt gggacggctc gatagccgct tcaccggcga
    19561 tgaactcacg cgcgtccgcg gggaaggcca gcagttcgac ccgtcgatgt cggtgatacg
    19621 ccccccgttt actgcgacct tcaataacta tgtccgcagc gatcttggat acaagaccga
    19681 ccttgaatac tatgtgctgg gcggtggcgt tactgactgg gattggggat ccgccggaga
    19741 agggtttccc gataccagcg aggcgctgcg cctggccttc gtgaaaaatc cctacatgaa
    19801 attgttcgtg gcttccggat actacgacct cgcgacgcca tacttcgcga ctcagtacac
    19861 gctgagccac atgaacctca acacgcagca gcacgccaag atcacgctgg gttattacga
    19921 cgccgggcac atgatgtaca tcaagagcga cgctctggcg cggctgaagc aggaggtgtc
    19981 agggtttctc aacggtgctc tgcaatagcg tggttcgaaa agcttagtcg taccgctcga
    20041 agacgatttg cttgcggtcc cagccgagtt cgtccttgag tagggcgcgg ttggcgtcaa
    20101 ccatctggtg cagcccgcag atgtaggccc gcatgtcaga gcgaccggtc gcaatctcac
    20161 gcacatggtc ttgaacgtat ccgcgcagtc cctgccactc atcgccacca cggctgagcg
    20221 ttgggagata gtggaagttg gggtgcgcct tttcaatggc gagaaactct tcgcgataat
    20281 agagtccatc ttcgtggcgc gtgccataga ccagccagaa ttcgtgaccc tggttgcgct
    20341 cggggtgctc aaagagccag tgtacgattc cgcgaaacgg tgcgatcccc gtgccggtgg
    20401 cgacgaacac cgcatcgtgg cggggctcgc gtagcgtgaa catcccgtgc ggcccgtgaa
    20461 actcgacggc cgtaccttct tccagatcgc acagccaatt cgacaggaat ccctgttcca
    20521 cgcgattcag gcaaaggtca aattgcgcgc ccgcgcgcgg cgtggaagcg agcgaatagg
    20581 cgcgggtatg cgtcttgccg tccggcttcg gctgctggat cgatacgaac tggccgggaa
    20641 cgaagcgaaa ttcgttcacg ccctccaccg cgaactcaag atgcttggtc tggtccgaga
    20701 ggaagacgga ccgcacgagg cgcgcggaca aaagcttgcg ctggccgggt tggccgacga
    20761 cagggtcggg cactgccagt gcggacacta ctggattgga atcgctcaca gtccttagat
    20821 gcccccgtcg tgcgattggc atcacgacca atcgctgatc gccgaaaatt ggatggtgaa
    20881 ccagagaagg cagccaagtt agccgccttt gcgacttcgt tcttgcggat ctactccact
    20941 gtgaccgact tcgccaaatt gcgaggttgg tcaacgtcgc atccccgacg gaccgcgatg
    21001 tggtacgcca gcaattgcag cggcacgact tccagaatcg ggactagcaa ctcgggcgcg
    21061 gccggcacat agagaacatg gtccgccgat tccttgatct cctcatcgcc ttcggttgct
    21121 acagcaatca ccgttcccga ccgcgccttc acttccttca ggttggaaat cgtcttctcg
    21181 tagcgcagca ccgagccagg gtcatcgaga tcgcgcgtgg ccatgatgac caccggaaga
    21241 tcttcatcga tcaacgcatt gggcccgtgt ttcatttcgc ccgccgggta gccttcggca
    21301 tggatatagg aaatttcctt catcttcagc gcgccctcga gggcgatcgg atagtgtatc
    21361 ccgcgtccca ggtagaggaa atcatgcgca cggtggtacc gcttggccaa ctcttcggtt
    21421 tcgtcatcgc tgcccaggag agcttcgagt ttgccgggaa tgagcgtgag ttcggtcagc
    21481 agtttcttgg cctcttctgg gctgattgtt ccccggacct cgccgagata caccgcgaaa
    21541 aggaacaagg cagtcagttg cgcggtgaaa gccttcgtgg aagcgacacc gatctcgggt
    21601 ccggcatggg tgtaaatcgt gccattggat tcgcgcgcga tcatcgatcc aacgacgtta
    21661 cagacgctca gggttttcga acccttggcc cgcgactctc gcatggccgc gatggtgtcg
    21721 gcggtctcgc ccgactgggt gatcagaatg gtgagatctt caggaccagt cagtggatcg
    21781 cgatagcgaa actcgctggc ataatcgacc tcaaccgtga cttttgccag gcgctcaatc
    21841 atgaacttgg cggccaatcc ggcgtgccaa ctggtgccgc aggcggcgat atagacacgt
    21901 tgcagtttgc gtaggtccga ctcggagatg tccatctcgt cgagaaagac cttgccggta
    21961 tcgagcgaga cgcgtcccat cgtggtgtcg cgcacggcgc gaggctgctc aaagatttcc
    22021 ttgagcatga agtgcttgaa gccgcccttc tcggccatga tcgggtccca cgtgatgtgc
    22081 tgcacctggc gcgtaatggg atggccatcg aagtcagtca actgcacgcc gtcggctgtg
    22141 atcactgcca aatcgccgtc gcccagaaag aacaggtcac gcgtgtgata aaggattgca
    22201 ggcacatcgc tggctacaaa gtattcgtca tcccccagcc caatcacggc gggcggcccg
    22261 ttacgcccgg cgacaatctt gttcggctcg tccgacgaga tcacggcgat cgcgaaaacg
    22321 ccagtcagtt gcttgaccgt tcgccgcacc gcttcttcca gcggaatctt gtgatcggcg
    22381 tctgccagcg atttctcgat gagatgggcg atgacttcgg tatcggtctc ggtgcggaac
    22441 ttgtggcctt cttcgatgag cttcttcttc aagctcatgt agttttccac gatgccgttg
    22501 tgcaccacga cgatcttgcc cgtgcagtcg cgatgagggt gcgcgttctc ttccgtagga
    22561 cgtccgtgcg tggcccagcg ggtgtggccg atgccgtagg ttccgtcgag tggcttgagc
    22621 tggatgacct cttccaggtt gcgcagcttg ccttccgcgc ggcgcacctg cagcccttcg
    22681 ccattaccag ccacagcgat gccggctgag tcatagccgc gatattccaa acgacgcagg
    22741 ccgtcgatga tgaccggaac gacccgcttc ttcccaacgt aaccaacaat gccacacatc
    22801 ttttgtgctc caggaaactg gctatgtccg catttcgcgg tccagtgaat gccattgccc
    22861 gcgttctcga gcggatcgcg agtgacaatt aaatcttatc cgatctcggc gattgatggg
    22921 aggacaggct agttacttta gtacccacga gctgctgcca caacggccca atccgattgg
    22981 atgcagattc cgctaactag gtctcaagcg tgaagcgata ttcctcgatc accggattgg
    23041 tgagcacctc gcgggcaatg cgctcgactt cagcctgggc gtcctcgcgc ccaatgctgt
    23101 cgtccaacgc gatctcgaag tatttacctt ggcgcaagtc gttgacgccg cgataaccca
    23161 tctttttcag ggcgccatgg atggttttgc cttgaggatc gagcacactt ttcttgagcg
    23221 aaacgtaaac gtaggctttc atagattagt ccttattata acgacactgt cgcggttatt
    23281 tcagactacg tttcggtggc tcacgacgac ggcgtcttaa acgcgtcgtc gatctcgacc
    23341 tggtatccct tgaccagacg attggtctcg ttggccattc cgacaacgga catcaactcg
    23401 tgaaacacgg caggagtcat cccggccttg cgggctgacg ccgtgtggga agcaatgcag
    23461 tagtcacatc cgttggtcac gctcactgcg acgtagatca tttccttgac cagcggatcg
    23521 attgcacctg gggccatgat ttgcttgatg ctctgccagg ttcgcttgag caacaccgga
    23581 tcgtgggcaa tcgccttcca gaagttgttg atccaatcga ccttgcgggt agccatgatg
    23641 tcgtcataca cggcgcggac ttccggtccg gcttccgcgt actcaactaa gccataggtt
    23701 gccatcggac tctcctcgct cagacgattt gtactttttg tttgtaggcc gcgacttcca
    23761 cattccacgc aaggtccttc tccatagtgg tgcgcaacgc caaagccgca gtgggttcgc
    23821 catgcaccag gtcggtctgc ttgggcgcag cctgaaatgt gcgcagccat tccagcagtt
    23881 cgggagtgtc agcgtggtcg ctgaactgtt ctaacgccgc cacctgggcg cgcacgggaa
    23941 ccatctgccc aaaaatcttg acctcagttg cattgttctt gatgtcgtac ccacgcgtcc
    24001 cctgtgcctg aaaaccgata aacagaatca tgtttttcgg atccggcagc cgctgcatca
    24061 aatggtgcaa gactcgtccc ccggtcgcca tgccgctgga cgatacgatg atcgacgggt
    24121 acgtcgactg gttgattttc ttggaatcgt cctgggtgag gtcaaagtag aaattggccc
    24181 agtccagtgg agacccgtat ttgtcgatga gcgccttcgt ctccgggctg aactcatccg
    24241 tgtatttcag gaagatttgg accgccttga tcgccatcgg gctgtcggcg tggatcggaa
    24301 cacgcggaat ctgctgggtc tccatcaatt ctttgagcat aaagagcagc ttctgcgtgc
    24361 gctcaaccgc aaaagcagga atcacaactg tgcctccccg ctgtacggtg gaacgaatta
    24421 gtgccgccag ttccggtctg ggatcgtcgt gtgggtgttc gcgattcccg tacgtgcttt
    24481 ccatcaccag gtaatcgcag cgctcgtgct ccgtcgggcc cgcatacaaa acccgtccag
    24541 gagagatttc gctgttgcga acgcgtccga tgtctccggt gaacagaaga cgcgaagtct
    24601 ttccctgaga ggtcagcgta atttccgcca tcgacgaacc tagaatgtgc gccgcgcgaa
    24661 cgaactgaaa cgagatctcg tcagaaaggc gcttcggctc attgaatttc accgccttga
    24721 atagagaaag acactgctgc gcctcctcca tcgtgtagag cggcaacgcc gggtcatgct
    24781 tcgacgtttt gaaccggtta tgaaaggccg cgtcttcttc ttgcaagtgt ccggagtcag
    24841 gcagcaggat gccgcacaaa tcgatggtgg ctggcgtcgc gtaaatcggg ccatggaagc
    24901 cttgcttcac caggcgaggt atccagccgg agtgatcgag gtgcgcgtga gtcaggatga
    24961 cggcgtcaat ttcgcgggcg ggaattggca ggtcctgcca attgcgttcg cgccattcct
    25021 tttgcccttg aaagagccca cagtccacca gcacctgaat gccacgtttg ccgctgtcat
    25081 cgccggtatt gatcaggtgc ttcgatccgg taacaactcc cgctgctccc agaaactgga
    25141 tgtaactcaa gctggcctct ttcgtgcgct ggtcgcgcga ctgcgatcca ccacgtttgt
    25201 agctttcaac cctgctggtt ggcaagtacc cggcgtctaa ggagccgggc tggccggaat
    25261 ttcgaaagta ttgtcgcccg agttgatctc cgggacacta ccatcgttca ccacgacctt
    25321 ggtcggaggg gcggggactt gtgaccttcc agaggccttc tggtgcgccc tgaccaacac
    25381 tctcacgact ctttcacccg aaggagtctg gatcacgacc gggacttcag cgccagcatt
    25441 tccgcggttt tcgacggtca cagtcaccag gacaatcttg ctgggatcgc cgagcaacgg
    25501 ccgcacatac gcgttctcca cgcggaaatc aggaagccca cgatttcgat acacccaatc
    25561 gtcgaagaac cactccaggt cgcgcttctt cccctcctgc aacaggttct gaaaatatgc
    25621 tgggttcaag tcagcccccg gacggtaggt ggccagcgcg tgttgcaatg cctcatcgcc
    25681 catcatgtca cgcagcatcc agaagacgaa gctgcccttg ccgcgcagca gaatctcgtc
    25741 ggaggtattc aatagtgtgt cgtccgccgc gtgactgtca gtggacttcg ccgcggagcc
    25801 cggttcagcg ctcgccgatt cttcagcctt caccagcgga gccagatact cgtcgagaaa
    25861 ctgtaacgcc cctttgcgtc gcgaacggcg ctccacgctc actacctgca gaaagcgttg
    25921 cagcccgtcc tcgatccagc gatggggaga agggaagcgt gctgcgacct gaaccggcag
    25981 cagcaggagc gaaagcgtgg cggtctcgga ctggcgcagc ggcgtgaaca acacggcgcc
    26041 gttttggtag gggctggtgt tcggatccgt cagttcgatc accgtgggca cttgcaccgc
    26101 cgggttcagc cattcatgca acacggattc gttggcttcg gcagcggcag cgtaatcccg
    26161 agccagtgat attttttcgg gagtgtgaaa gagcgttgcg ccggaatgtt c
//
LOCUS       DASRKK010000389         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_420756, whole genome shotgun sequence.
ACCESSION   DASRKK010000389 DASRKK010000000
VERSION     DASRKK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420756"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFC15_08805"
     CDS             <1..797
                     /locus_tag="VFC15_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM10295.1"
                     /translation="HNKHDGTFEEIAVDAGVAYGQNGESTSAMGPVFADIDNDGKLDL
                     WVTDSKYNRLMRNTGKMQFDDIGAASGIAPVTGQYVSWGTGIYDFDNDGILDILTFHG
                     GLIHLIPEEHSIFRGLGNGKFVDVSSQAGSVFDVKTVARGACFGDYDNDGKIDAFLVN
                     LGAPATLFHNVSANAGHWITIHLKGTKSNRDGIGARLEVTADGKKQIAERVSSSGYLS
                     QDDGRVHFGLGAATKIEKLTVRWPSGREQVLQNLSVDRILTVEEPK"
     gene            794..2689
                     /locus_tag="VFC15_08810"
     CDS             794..2689
                     /locus_tag="VFC15_08810"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZM10296.1"
                     /translation="MRLRPIRVVPGSAAIVAVMAVAVVFAFLAFTLSAKAKPDSDEAR
                     YLNPREMVLSPDGHRLYVMCEGSDEVRIVDASSGKVLQVVAVGHMPRGISLSPDGRQI
                     YVANSWDDTLSVIDASSLKVVRTLATGFEPNSVVSDREGKTLYVANRLSSDISVIDLE
                     SGQEIKRLLAGRGASYLALSADGKLLYCTHVYPRIGAHRTPPESEVTVIDTDRQRVIE
                     RKPLHNVGGVFHIALSADGKLGVAPQLRPKNLVPLAHVEHGWAFGDSLALFGEDVGGG
                     VVQVPIDELDRYFAMPYAVAITPDKSKLYVSSSGADSITVIDIPKMLSYVHAASGPIA
                     NDLSASANYVLTRIPVGRNPKGLLLSGDGKRLYVANRLDDSISVIDTNNDVVVSSIDL
                     GGKQDMTSLRRGERIFYTARFAFQGQFSCANCHIDATFDGLQWDLEPDGFGVDIVDNR
                     SIEDLSGTEPFKWNGGNPNMATECGPRTEKYFYRSQSYNSAELADLVSYVMALPLRPN
                     RYRLPAGELTPAQERGKAIFERKTNKFGKAIAESNQCAYCHSGPKYTNQKLADVGTGK
                     KTDRSPLVDVPQLTNVAYSGPYLHDGSARSMEEIWTIFNPKDTHGFTNDLTKDELNDL
                     IEYLRTL"
     gene            2686..3747
                     /locus_tag="VFC15_08815"
     CDS             2686..3747
                     /locus_tag="VFC15_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10297.1"
                     /translation="MKRAWALLLFVLMSVAAYAATPDMPRFRFENFTTANGLPDNHVF
                     TVLVDGDRVWAGTENGLALYEAGKWKVFTTADGLAHRAVLSLALDKSTGDLWVGTMGG
                     LSHISAGRIDTYTQLNSGLSNDVVYGVGVQGDYVWTATAAGASRLNTRTGEWSLYNER
                     NAPMGEIWAYAVSTGADRVYYAVWGGGVLEYTLATGRWKDYTDPDGENELVLFKDQGL
                     IHKITTSVSYVDKVLWVATYFGDSHYDGRSWHNFMVKDSGLPSNFTNQVKGLDATRAW
                     FCTDKGLAYYDGVNWAVYRPSLTNGKPEMYVRDAAGAITPIVVETAPAHNYVLAVDLQ
                     GDDIWVATAKGLSHGIRQK"
     gene            3776..4258
                     /locus_tag="VFC15_08820"
     CDS             3776..4258
                     /locus_tag="VFC15_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZM10298.1"
                     /translation="MSVQELLSEVATAAVEKRAITNLEVLNEAYDYAKPSSFVRGMRL
                     DLNGLVILLISGTASIDDKGETVHVGDFRAQCRRTFKNITGLLEAEGATWHDIVRTTC
                     YLRDIDRDYAAFNEERTAFYNEQQLNPVPASTGIQAKLCRPELLVEIEAIAMFKKLQS
                     "
     gene            4306..6342
                     /locus_tag="VFC15_08825"
     CDS             4306..6342
                     /locus_tag="VFC15_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM10299.1"
                     /translation="MKTELRAVHVVVRVGLVAAMLFPLVVTSVLAQTGASAQEPNLCA
                     CGSHPPKPRPQRTMAPYANEPKDLQPYSKFAEPYDHHYIQTNVYSGAARDVPDPDMKD
                     LTEIRIGFLGPIDTNPDQVFGQRMLNGAQLAVDQANARGGYCGKPFKLMPRNDYDNWQ
                     AKAVYGDNRPTSQDIWGSASNQTVKMVYDDKDWAIFGSISSESTHILLRVALRAEIPI
                     VNSASTDPTIPETYIPWYFTDLQDDRVQANTLARYIYDELKLKRVALLRINNRYGRFG
                     SPKFKDASQRLGHPVVIEQRYPAGTTDFTRELRAIQASRADGIVLWNDQEQTAMILKQ
                     MHELSMKQLVFGSYRTIGDDLIAQAGDQAEGFEAVFPYDASRTDPRWQKFNSDYAATY
                     REKPEQFASLAYDAMNVLLGSVCEAGLNKGRIMDSLAQVYEYDGVTGHMVFDTNSKNI
                     APMYLARIHNGRIEYRPASMEPIARQAEPKPAPPSAAPASESTPPKIAPVPYARVGED
                     GVSFSGPSEADSTTGVVRIAVFGPHADQLVRSPEVLETLTRLNAGHQQWSLIAIPSDV
                     QWGKATSELVRAVFDQDVLGIVALDRDSSHLAEQFGVKTFVPVIAISSDHALTSTNIP
                     WIFRMSEGTSLDQALQALVAAEKQAGPSRARLREVLASGTQVAGVRFQSTGEPR"
BASE COUNT         1444 a         1836 c         1998 g         1312 t
ORIGIN      
        1 tccataacaa gcacgatgga accttcgagg agatcgcggt ggacgccggc gtggcatatg
       61 gacagaatgg cgagtcaacc tcggccatgg ggccggtatt cgccgatatc gacaacgacg
      121 gcaaactcga tctatgggta accgactcga aatacaaccg cctgatgcgc aataccggaa
      181 agatgcagtt cgacgatatc ggcgccgcgt cgggcatcgc gccggtgacc gggcagtatg
      241 tgagctgggg tacggggatc tacgacttcg acaatgacgg catcctcgac atcttgacct
      301 tccacggagg cctgattcat ctgataccgg aggaacattc gatcttccgc gggctgggga
      361 atggcaagtt tgtcgatgtc tccagccagg ccggttcagt gttcgacgtg aagacggtgg
      421 cgcgcggcgc gtgtttcggc gattacgaca atgacggcaa gattgacgcc ttcctggtga
      481 acctgggagc acccgcgacg ttattccata acgtctcagc caatgctggc cactggatca
      541 cgatccatct gaaaggtaca aagagcaatc gcgacggcat tggggcgcgt ctcgaggtga
      601 ctgcggacgg gaagaaacag atcgcggagc gggtctccag ctccgggtac ctatcgcaag
      661 acgatggccg ggtgcatttc ggattaggtg cggcaaccaa gatcgagaag ttaacggtgc
      721 gttggcccag cggacgcgag caggtgctgc aaaacttatc agtcgatcgc atcctaacgg
      781 tggaggagcc gaagtgaggc tgcgccccat tcgcgtcgtt cccggatctg cggcaatcgt
      841 cgcggtcatg gccgtcgcag tcgtgtttgc atttcttgcg ttcaccctgt ctgcgaaagc
      901 gaagccagac tccgacgaag ctcgttacct caatccacgg gaaatggttc tctcgccgga
      961 tggacatcgg ctgtacgtga tgtgcgaggg cagcgatgag gtgcgcatag tcgatgcaag
     1021 ttcaggaaaa gtcttgcagg ttgtcgccgt gggacacatg ccgcgcggaa tttcgctgtc
     1081 tcccgacggc cggcagattt atgtggcgaa ctcctgggat gacacgctgt cagtgatcga
     1141 cgcctcatct ctgaaagtgg tacgcacgct ggcaacgggg ttcgagccca acagcgtggt
     1201 gtctgatcgc gaaggaaaga cgctgtacgt ggccaaccgg ctcagcagcg atatctcggt
     1261 aatcgatctc gaaagcgggc aagagattaa gcgccttctg gctggccggg gagcaagtta
     1321 cctggcgctg tcggccgacg gaaagctcct ttattgcact catgtctatc ctcgtatcgg
     1381 cgcccaccga actccgcctg agtctgaagt cacggtcatc gacactgatc gacaaagagt
     1441 catcgagcgc aagccgcttc acaatgtggg tggggttttt catatcgcac tctctgcaga
     1501 cggcaagctt ggcgtagcgc cgcagcttcg tcccaagaac ctggtaccgc tggcgcacgt
     1561 cgagcacgga tgggcattcg gcgattcgct ggcactgttt ggcgaagacg tgggcggcgg
     1621 tgtggtgcag gtgccaatcg atgagcttga ccgctacttc gccatgccct atgcggtggc
     1681 gatcacacct gacaagtcga agctgtatgt gagcagctcg ggtgccgatt cgattacggt
     1741 gatcgatatc ccgaagatgc tcagttacgt gcatgcggcg agcgggccta tcgcaaatga
     1801 tttgtcggct tcggcgaatt atgtgctcac aagaattccg gtgggccgca atcccaaagg
     1861 ccttctgctg tcgggcgatg gcaagcgcct ctacgtggcg aaccgcctgg acgacagcat
     1921 cagtgtaatc gacaccaaca acgacgttgt cgtgtcctcg attgacttgg gcgggaagca
     1981 ggacatgacg tccctgcggc ggggcgagcg cattttctac accgctcgct tcgcctttca
     2041 ggggcaattc agttgcgcca attgtcacat tgacgccacg tttgacggtc tgcaatggga
     2101 cctcgagccc gacggcttcg gcgtggacat tgtcgataac cgctcgatcg aagacctgtc
     2161 ggggacggag ccgttcaagt ggaacggcgg caatcccaac atggcaactg agtgtggccc
     2221 gcgcactgaa aagtatttct atcgctcgca aagttataac agcgcggaac tcgctgactt
     2281 ggtgtcttac gttatggcgc tacccttgcg gccaaaccgt tatcgattgc cagccggcga
     2341 gctgacgccg gcgcaggagc gcggcaaggc gatctttgag cgcaagacga acaagtttgg
     2401 caaggccatc gccgagagca accagtgcgc atactgccac agcgggccga agtacaccaa
     2461 ccagaagctc gcggatgtgg gcaccggcaa gaagacagac cgctcgccgc tggttgacgt
     2521 gccacagctc accaatgttg cctactctgg accatacctt cacgacgggt cggcgcgatc
     2581 gatggaagag atttggacga ttttcaatcc caaagacacg cacggattca ctaacgattt
     2641 aaccaaggac gaactcaacg acttgatcga gtatttgagg acattatgaa acgggcctgg
     2701 gcactcctgc tctttgtgtt gatgtcagtc gcggcatacg cagccacgcc agacatgcct
     2761 cgcttcaggt ttgagaactt caccacagcc aatggccttc ctgacaacca tgtattcacc
     2821 gtgctggtgg atggcgaccg ggtgtgggcg ggcacggaaa acggactggc tttgtatgag
     2881 gccggcaagt ggaaggtgtt cacaacggcc gacggattgg cgcatcgcgc agttttgtcg
     2941 ctggccctgg acaagagcac gggtgatttg tgggtgggta cgatgggcgg cctgagccat
     3001 atttccgctg ggcgaatcga cacctacacg cagctcaact cagggctgtc caatgatgtg
     3061 gtgtatgggg tgggagtaca gggcgattat gtatggacgg cgacggccgc gggcgcaagc
     3121 cggctgaata cacgcacggg cgagtggtct ctctacaacg agcgcaatgc tccgatgggc
     3181 gagatctggg cttacgcggt gagcactggc gcggacaggg tgtattacgc ggtttggggc
     3241 ggcggcgtgt tggagtacac gctcgcgacc gggcgctgga aggactacac cgaccccgac
     3301 ggtgaaaacg aactggtgtt gtttaaggac caggggttga tacataagat cacgacctcg
     3361 gttagctatg tggacaaagt cctttgggtg gccacctatt ttggagacag ccactacgac
     3421 ggacgaagct ggcacaactt tatggtcaag gacagcgggt tgccgtcaaa cttcaccaac
     3481 caagtgaagg ggctcgatgc cacgcgggct tggttctgca ccgacaaggg tctggcctac
     3541 tacgatggcg tgaattgggc ggtgtatcgg ccgtcgctca ccaacggcaa gcccgagatg
     3601 tatgtccgcg acgcggcggg agccatcacg ccgatagtgg tggagacggc tccagcacac
     3661 aattacgtcc tagcggttga tttgcaaggg gacgacatct gggtagcgac cgccaagggt
     3721 ttgagtcatg ggattagaca gaaataagga agaccatttt caaggagaga atgtcatgag
     3781 tgtccaagag cttttatcgg aagtggcgac agcggctgtg gagaagcgcg ccataaccaa
     3841 cctcgaagtg ttaaacgaag cttacgacta cgccaagccc agctcttttg tacgcggcat
     3901 gcgcctggat ctcaatgggc tggtgattct gctgatctct gggacagcca gcatcgacga
     3961 caaaggcgag accgtacatg ttggcgattt tcgggcgcag tgccgccgca cattcaagaa
     4021 catcacgggg ctgctcgaag ccgagggcgc gacctggcac gacatcgtta ggacaacctg
     4081 ctatctgcgt gacatcgacc gcgattatgc cgcgttcaac gaagagcgca ccgcgttcta
     4141 caacgagcaa cagttgaatc cggtgccggc atccacgggc atccaggcca agctgtgccg
     4201 tccggaattg ctggtcgaga ttgaagccat cgccatgttc aagaagcttc agagctagcg
     4261 ctgtgggaat cagcaagacg gccgcgaggc cgtgaggact tggatatgaa gacagagtta
     4321 cgggctgtcc acgttgtggt gcgtgtggga ctggtagccg ctatgctttt tccgctagtg
     4381 gtcacctcag ttctggcgca gaccggcgcg tccgcacagg aaccgaatct ttgcgcctgt
     4441 ggcagtcatc cgcccaagcc gcggcctcag cgcacgatgg cgccgtacgc caacgaaccg
     4501 aaagacctgc agccgtactc gaagttcgcc gaaccctacg accatcacta tatccaaacc
     4561 aacgtctatt ccggggctgc acgagacgtc cccgatccgg acatgaaaga cctcacggaa
     4621 atacgcattg gctttttggg accgatcgac accaaccccg accaggtgtt tggtcaacga
     4681 atgctgaacg gcgcacagct tgcggtggat caggcgaatg cgcgtggcgg atattgcggc
     4741 aagcctttca agcttatgcc gcggaacgac tatgacaact ggcaagcgaa agcagtctac
     4801 ggagacaacc ggccgaccag ccaggatatc tggggatccg cgtcaaacca gaccgtgaag
     4861 atggtgtacg acgacaagga ctgggcgatt ttcggctcga tcagttcgga gtcaacccac
     4921 attctgctgc gcgtggcgct gcgggctgag atacccatcg tcaactcagc ctccaccgat
     4981 ccgacgatcc cagaaactta cattccctgg tacttcaccg atctgcagga tgaccgggtg
     5041 caggcgaata cgctggcccg ttacatctat gacgagttga agctgaagcg agtggcgctg
     5101 ttgcgcatta acaatcgcta cgggagattt ggctcaccga aatttaagga cgcgtcgcag
     5161 cgattggggc atccggtcgt tattgagcag cgttaccccg ccgggaccac ggactttacc
     5221 cgcgaattac gtgcaattca agcctctcgc gcggacggca tcgtgctgtg gaacgaccag
     5281 gaacagacgg cgatgatcct gaagcagatg catgaattga gcatgaagca gctcgttttt
     5341 gggagctacc gcacgattgg cgacgatctt atcgcgcaag ccggagatca agctgaagga
     5401 ttcgaagcag tctttcctta tgatgcctca cgaaccgacc cgcgttggca gaaattcaac
     5461 agcgactatg cggcaacata ccgcgagaag cctgaacagt ttgcttcact ggcctacgac
     5521 gcgatgaatg tgctgttggg ctccgtctgc gaggccggcc tgaacaaggg ccgcatcatg
     5581 gactcactgg cacaggtcta tgagtacgac ggcgtgaccg ggcacatggt cttcgacacc
     5641 aacagcaaga acattgcgcc gatgtatctg gcgcgaatcc ataacgggag gatcgagtat
     5701 cgtccagcct cgatggagcc catcgcccgg caggcggaac cgaagccagc gcctccgtct
     5761 gctgctcctg cttcagaatc tacaccccca aagattgcgc ctgtgcccta tgcccgagtc
     5821 ggcgaagatg gtgtttcgtt ctcgggtcca tccgaagctg attcgactac aggtgtggtg
     5881 cgcatcgcgg tcttcggccc gcacgctgat cagctggtgc ggtcgccaga ggtgctggag
     5941 acgcttacga ggctcaatgc cggacaccaa caatggtcac ttatcgccat cccgtctgac
     6001 gtgcagtggg gaaaggctac cagtgagttg gtacgtgcag tcttcgacca ggatgtgctc
     6061 gggattgtcg cacttgaccg cgattccagc catctggccg agcagttcgg ggtaaagacc
     6121 ttcgttccgg ttattgccat ttcgtcggac catgccctga cttccacaaa catcccatgg
     6181 attttccgca tgtccgaggg cacatcgctc gatcaagcgc tgcaggccct ggtcgcagcc
     6241 gaaaaacaag cgggcccaag ccgcgccagg ctccgtgagg tacttgcgtc gggcacccag
     6301 gtcgccggtg tgcgctttca gtcgacgggc gagcctcggt aattgcaggg cgagtgctcg
     6361 cctgggcttt cacttgcggg gcctgggccc agaaatctcg cagcttttct cctccaccct
     6421 catctactat ctgcacctcc gtgacggtga ggagcgagta tggggacaga cgcagtgcca
     6481 actacatcac cagaacttcc cgaactgccg ctccaagcat ggcaagccac ctgcgatacg
     6541 ctgcacatgt ggacgcagat cgtgggcaaa gtccgcaagg ccctcagccc
//
LOCUS       DASRKK010000390         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_224563, whole genome shotgun sequence.
ACCESSION   DASRKK010000390 DASRKK010000000
VERSION     DASRKK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224563"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFC15_08830"
     CDS             complement(<1..444)
                     /locus_tag="VFC15_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZM10300.1"
                     /translation="MIRLIRDLVRPYRGTLLIILLAMMVETAMSLAGPWPLKIIIDNV
                     IGSHKLPHWLDQAVRPMLEGGGKLKVAALAALATEIIALLGALASYIDNYYTESVGQW
                     VANDLRLRTYHHLQRLSLAYYDTHQTGTLLSTITTDIQTIQGFASS"
     gene            complement(640..1116)
                     /locus_tag="VFC15_08835"
     CDS             complement(640..1116)
                     /locus_tag="VFC15_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10301.1"
                     /translation="MTKTYFITASAYMHQNLFQRTETADLLLTTLFRYRDTGEFLVHE
                     FVVMPNHMHLLLSVDDDHAIGRGVQLIKGGFSHAMGKAGLKLKAVWQPSYYEHRVRDA
                     EEYGRMRAYIHDNPVRRALVRTAEEYPYSSARTTWHIDEVPERLKPENQETVLTRR"
     gene            complement(1304..1891)
                     /locus_tag="VFC15_08840"
     CDS             complement(1304..1891)
                     /locus_tag="VFC15_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZM10302.1"
                     /translation="MSLRNPAASCSRGFQVFFLVLLVLLVSLGAQAQKINVSYDKSLD
                     FSKFKTYAWEPHGAVAHPMLAADIVGAIEDEMKAKGLQLTTDKPDLVIQVYGSIDQDS
                     TLYSNDPLYMATGGIPPFDPSAFGPALAGTWGNTTVTIHKGQLVVDLLDVSTKKLVWR
                     GMATQNLAAHDPKKLLSQVNGSIAKMFKQYPAGKG"
     gene            complement(2114..>3055)
                     /locus_tag="VFC15_08845"
     CDS             complement(2114..>3055)
                     /locus_tag="VFC15_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZM10303.1"
                     /translation="KERKIMSTDRIVKFAFVVSLSLAALALLCGVAGAQSAQLQGVIN
                     GRSGATMTVQSQDLGNVVVLLTDNTQVEDVSGLFHARKKQMGMTALVPGLQVQVKGSY
                     NAQNQLVADTVKFNGKNLQTATDIQAGVTPVEQQEQAQKAQAAKQEAQIQKQEQAMTQ
                     QQQEMAAAQAKIAANKAAIAAVNKRFGELDDYNIWDEVTVLFGNGQVAVDPQYKPKLL
                     ALCQKAKTVTGYVIQVKGYASAVGSAALNQKLSQERAANVTDFLDQQCHIPLTNVLAP
                     GAMGTSKQVAPDNTAEGQAENRRVVVRVLQNKGIAGT"
BASE COUNT          568 a          906 c          880 g          701 t
ORIGIN      
        1 agacgaggca aaaccctgga tcgtctggat gtccgtggtg atggtactga gaagagtgcc
       61 ggtctgatgg gtgtcgtagt aggcgagcga gaggcgctgc agatgatggt aggtgcgcag
      121 gcggagatcg ttcgccaccc attgcccgac actctcggtg tagtagttgt cgatgtagga
      181 ggcgagcgct cccagtagcg caatgatttc ggtagccagc gccgccagag cggcaacctt
      241 caacttgccg ccgccctcca gcatgggccg gactgcttga tcgagccagt gcggcagttt
      301 gtggctgcca atcacgttgt cgatgatgat cttcagcggc cacggccccg ccaggctcat
      361 cgctgtttcg accatcatgg cgagcaggat gatcaggagg gtaccgcggt aggggcggac
      421 taggtcgcgg atcaggcgaa tcatgcggtg gcctcgcgct caaagataca tcagccgcgg
      481 cgcggcgcgg tatgcaaacg acatgctcgc gtgtcgctaa atcaggagtg tttcatttgc
      541 gccggggcag aggaaccagg gacgctctgc gaacagcatc gtgtatttcc cgggctgggc
      601 gagattgcca aaaaaatgac gttcgggtag tgcggcgctt tagcgccgcg ttaacacagt
      661 ctcttgattt tcaggcttta gcctctcggg tacctcgtct atatgccacg ttgtcctggc
      721 cgacgaatat gggtattcct ccgccgtcct taccagcgct cgccgcactg gattgtcgtg
      781 aatgtacgcc cgcatgcggc cgtattcctc cgcgtcccgc accctatgtt cgtaatagct
      841 cggctgccat actgccttca atttcagccc agccttcccc atcgcgtgcg agaagcctcc
      901 tttgatcaat tgcacgcccc ggccgattgc atggtcgtca tcgaccgaca acagcaggtg
      961 catatgattc ggcatgacga cgaattcatg aaccaggaat tcaccggtgt cacgatatcg
     1021 aaagagtgtg gtcagcagca gatcggccgt ttccgttcgt tggaataggt tctggtgcat
     1081 ataagccgac gcggtgatga aataggtctt ggtcatgaag taccttgggg ctaaagccct
     1141 tccgtaacaa tcccgcttac gcggcgctaa agcgccgcac tacccaagct gccctcccct
     1201 cgccacacta cacgggtcgc acttccctcg cggcaccacc cgagctgcct tcccaccgca
     1261 ctacccgccc taaacgcctg gctctacccg catccttgct tccctacccc ttgccggcgg
     1321 ggtactgctt aaacatcttg gcgattgaac cattgacctg gctcaacagc ttcttaggat
     1381 catgggcggc caggttctgc gttgccatgc cgcgccatac cagcttcttg gtgctcacat
     1441 cgagaagatc gaccaccaac tgccctttat ggatggtcac ggtcgtgttg ccccatgtcc
     1501 ctgccagcgc cgggccaaat gcgctgggat cgaagggtgg aatgccgccg gtcgccatgt
     1561 agagcggatc gttcgagtac agggtgctgt cctgatcgat ggagccgtac acctgaatga
     1621 caaggtcggg cttgtctgta gttagttgca gtcccttggc cttcatttca tcttcgatgg
     1681 cccccacgat atcggcagcc agcatgggat gcgccaccgc accgtgcggc tcccaggcgt
     1741 aagtcttgaa cttggaaaag tcgagagact tgtcgtagct gacgttgatt ttttgtgcct
     1801 gcgcgccgag ggagacgagc agcacaagta agacaaggaa gaatacctgg aagcctcgag
     1861 agcaactcgc cgctggattt cgtaaactca tgcactcctc cgtcgacctg agcttgaaca
     1921 ctttctgtcg atcaaggcca taagccccaa aaaaacgacc ggttgacctg cgctggccaa
     1981 ctctcggtgg tgagagcctg ggccctgtgg cggacagcgt cgcgcggcgt cggtcttgcg
     2041 ggttccggca tttggcctcg ggtgtaagaa acggggccga atgttcgggc cccggtttct
     2101 gtaacgacta tgtttatgtt ccggcgatgc ccttgttttg cagcacgcgc actacgacgc
     2161 gccggttctc agcttgtccc tcagccgtgt tgtcaggcgc cacctgtttg ctagtgccca
     2221 tcgcgccggg agccaggaca ttagtcagag gaatgtggca ctgctggtcc aggaagtcgg
     2281 ttacgttggc cgcccgctcc tggctcagct tctggttcaa ggcggcagag ccgacggctg
     2341 atgcatagcc ttttacctgg atcacgtagc cggtaactgt tttggccttt tggcacagcg
     2401 ccagcaattt aggcttgtac tgcgggtcaa cggcaacctg gccgtttccg aagagtaccg
     2461 tgacctcatc ccagatgttg tagtcgtcca gctcgccaaa acgcttgttg accgcggcaa
     2521 tggccgcctt gttggcggca atcttggcct gcgccgcagc catttcctgc tgttgctgcg
     2581 tcatggcttg ctcttgcttc tggatctgcg cttcctgctt ggccgcctgc gccttctgcg
     2641 cctgttcctg ctgctccacc ggagttacgc cagcttgaat gtcggtagcg gtttgcaggt
     2701 tcttgccatt gaacttgacg gtgtcggcca ccagttggtt ctgggcgttg taggagcctt
     2761 tcacctggac ctggaggccg ggcaccagag cggtcatgcc catctgcttc ttgcgagcat
     2821 ggaacaggcc cgatacatct tccacctgcg tgttgtccgt gagaaggacg accacgttgc
     2881 ccaagtcctg actttgaacg gtcatggtgg cgccactccg tccgttgatg actccctgga
     2941 gttgggcgct ttgcgcgcca gcaacgccac aaagcagcgc cagtgccgcc aaggacagac
     3001 ttacgacgaa tgcaaactta acaatcctat ctgtgctcat gatctttctc tcctt
//
LOCUS       DASRKK010000391         9338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_518839, whole genome shotgun sequence.
ACCESSION   DASRKK010000391 DASRKK010000000
VERSION     DASRKK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518839"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="VFC15_08850"
     CDS             complement(<1..1861)
                     /locus_tag="VFC15_08850"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HZM10304.1"
                     /translation="MATAASDLRPLPSSLMGGRTQSTADEITVLGLAARGFRLFPVEK
                     RGKRALIKKWTERATCDAESLRAWMQAHPGCNWGVACGIDSRVFVLDIDGTEGQAAIR
                     ALSERHGHDWMKTLRVKTSRGAHLYFCYPDGPTIRNSTSKLAPGLDIRGTGGYAVVPP
                     SVHSSGHRYDWENENVPIARAPEWLLELVRARAQTSATLAARVGAGLIAEGQRNATLL
                     SDAGAMRRRGMTPKAIEAALLAENAGRCQPPLPESEVREIVRSVSRYAPAPEPTNDEH
                     ETAALLEDVRSFIQRFLVVSVAQAVTLCLFVLYTYSSEQFECAPYLQITSAEKRSGKT
                     RLLELLELLVRRPWMTARTSAAALVRKLAASHPTLLLDESDAAFSGDKEYSEALRGVL
                     NAGHRKGGRASLCVGRGCDIKVVDFDVFGPKVIAGIGRLPDTVADRAIPVQFLRKRPG
                     ETVERFRPRLVAQDATDLRDRLTGWATEDRLEILRRSFPELPEALSDRQQDVAEPLLA
                     VADLAGTEWASRARKALIELFGSRAAADESLGAKLLSDIREAFNGRDRLGSNELVTLL
                     VEMEGSPWPECNKGRELTANTLARLLRRFDIAPRTIRGETDTFKGYLRAFFEDA"
     gene            2190..2810
                     /locus_tag="VFC15_08855"
     CDS             2190..2810
                     /locus_tag="VFC15_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10305.1"
                     /translation="MALDYSKLVDRSFKYIGPKNPLRGLSGPDRTLLFSLLFIYRFEQ
                     TMPHRSKAVEAAEEMSKIAADSTALAQRIRSVMIEGKFALPLDPFLPGFRYLPSHLDL
                     FGKRLGTLMTHLAGKQGQKHKTTANQLLIMASELVRLKTGEPHDEHLAELLQAISPDA
                     EPGPDTDISGDSIKKKRRYMSKTYPAMYSSTISRLRRMASRSSRSD"
     gene            complement(2846..4051)
                     /locus_tag="VFC15_08860"
     CDS             complement(2846..4051)
                     /locus_tag="VFC15_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZM10306.1"
                     /translation="MALTDMRIKALKPKAKRYLVTDGRGLSVEVLPSGAMAWRYRYRL
                     HGKLEKVALGRYPDVSLKDARDKRDVMAGQVARGQSPAREKQLAKLALGTDSSVREFA
                     ERYYTDVVLRSLKDPSSVRRYLDKEIFPAFGNKALKDVTAADVQTLVFRKRDNGLEAA
                     AAAIRNLIKRLFDYAIVRGAAQANPALALPMRFITKARSRTRTLSPDEIRVYLRSLYQ
                     SNIRRQFKLALHLILLTLVRKSELLFAKWVDVDLESGEWQIPAANSKTGKPHVVYLST
                     QAADLFRELKPLAGESQWVLPSRSSPAKPFAHNAMNQALASMTFPMEPFTIHDLRRTG
                     STLLHEKGFSSDVVEKSLNHTIGGVRGVYNKAEYSDQRKEMLQFWADYVEGLASEKKV
                     LIARFGVAS"
     gene            complement(4238..5620)
                     /gene="mnmE"
                     /locus_tag="VFC15_08865"
     CDS             complement(4238..5620)
                     /gene="mnmE"
                     /locus_tag="VFC15_08865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZM10307.1"
                     /translation="MHLDDTIVATASPPGRGGIGIVRLSGAEARAIARPMLRLAHGHD
                     FEPGRAHFAELIEPSTGERIDEVVVTFFAKPHSYTTDDVVEISCHGSPVVLRHVVQMA
                     MAQGARLAEPGEFTMRAFLNGRIDLTQAEAVRDLIESQTLYQAQVAARQLEGALSKRL
                     QPAKQKLIELIAIMEAGVDFAEDDVAVIPDAQVLSRVAAIRAPLAELLASFAFGKVVH
                     EGLTLAIVGRPNVGKSSLFNRLVERERAIVTATPGTTRDLVSETVAIGGIPVRLVDTA
                     GIRPALDEAESIGVRKSMEALAEADMVLVVLDASHPAADDEARERDAELIALASQRLA
                     VVAQNKIDVANATAPLPAALTLPVVQTSAITGQGIAELRTAILRLLGGDSGAQQESGF
                     LTNVRQQKLVEDAIAALDQANAAVPLKIPHEMLLLDLYGALRPLDDITGATTADDILN
                     LIFSSFCIGK"
     gene            complement(5705..6121)
                     /locus_tag="VFC15_08870"
     CDS             complement(5705..6121)
                     /locus_tag="VFC15_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10308.1"
                     /translation="MNKTKLTAVSILLLTALAVGATPERSKGISMHLLPKRVADLSGS
                     KWGLTVSAADYLTPDAGSTTLQTGAEFRSFVRKQSSSVKQNGVWIVTTNLEAYSKSEN
                     TFLEQVMSICAADKIPLFICRASNLPDGWKRYDRLD"
     gene            complement(6167..6709)
                     /locus_tag="VFC15_08875"
     CDS             complement(6167..6709)
                     /locus_tag="VFC15_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZM10309.1"
                     /translation="MPIHDKIAAAKKISELLTTTFANGDFRLRYKITVDPQLGSDRDW
                     ERPEILVEISGADSPLVLENGAELLRAFENVALEMLRLAPNEHEKVSFDCQNFRSARL
                     QELRLAADVAAERVRKSGLPYAFAPMSSRERRIVHLALRDFDDLRTDSDGEALQRHVV
                     VYPKDYQGKPPAPPPLSRRR"
     gene            complement(6925..8709)
                     /gene="yidC"
                     /locus_tag="VFC15_08880"
     CDS             complement(6925..8709)
                     /gene="yidC"
                     /locus_tag="VFC15_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZM10310.1"
                     /translation="MAEYKNPQQEPGGDRRMLMVFAVTFALIIISQIFLFKDKSKPQP
                     KSAPDQTSQSNAPQSQSEAAGAPVEAVSKGRAPAVTKPTGKSAVTKSAPSEVETVVEN
                     DVFRIVFTNRGAQAKSWVLKKYKDEDGHPLDLVNPLAVKFGLPLSLYAYDEDLRNQVN
                     SALYVGSASGAITAPGELTYEYAQGDTTVRKTFQFDDTYVVSIETTVTQNGKPVQAYP
                     MWPAAFGDQSTGPSYASAKIEYMANDKVERLAPKKVSSGNTLRGPFNWAGSQDQYFAA
                     IFLPDNPDSAAMVTLHNSISVPKDLKNPDPNNLNHYDALGAAVGDVSGITRERLFVGP
                     KALHVLESVRSSTAPGQINGPDLRNVVDFGFFSLIARPLFLWLLWTHEHMASNWGVCI
                     MILTVIINLVLLPLRITSMKSALKMQKLQPQMKAIQEKYKKYPLRDPRRSEMNVEIGA
                     LYKREGANPAGGCVPLIIQMPFLFAFYSMLGVGIELRQAPFLWLHDLSSPDKIFILPV
                     LIVITTFLVQKMTPNAGMDPKQQQMMTLMMPLMIGFFSYSLPAGLSVYWTVGNFIAIA
                     QQYIMNRTGLGREMREEMEKRARKKASK"
     gene            complement(8712..8993)
                     /gene="yidD"
                     /locus_tag="VFC15_08885"
     CDS             complement(8712..8993)
                     /gene="yidD"
                     /locus_tag="VFC15_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZM10311.1"
                     /translation="MKQAVQFVLRCYQRVISPMLPHACRFVPTCSQYAIEAVEAHGVI
                     RGSLLAAARLLRCHPFARAGFDPVPPAIVKPGYGSPDNSLGNSHPLNGT"
     gene            complement(8990..>9338)
                     /gene="rnpA"
                     /locus_tag="VFC15_08890"
     CDS             complement(8990..>9338)
                     /gene="rnpA"
                     /locus_tag="VFC15_08890"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZM10312.1"
                     /translation="ADFQRVYQSGRRQFTGNMTVFFLRRGSGPQATGAGNTPRVGFTV
                     GKVLGGAVERNRIKRRMREAVRLSAQACEGPIDVVFNPRKSVSNMPFAELANEVTRAL
                     RLAAQRARTGEQK"
BASE COUNT         1884 a         2788 c         2705 g         1961 t
ORIGIN      
        1 aggcgtcctc gaagaaggca cggagatagc ccttgaacgt atccgtctcg ccgcggatcg
       61 ttcgcggtgc gatgtcgaac cgccgcaaaa gacgcgcgag tgtgttggcg gtaagctccc
      121 gccctttgtt gcattcaggc cacgggctcc cctccatctc tacgagaagg gtcacgagtt
      181 cgtttgaacc caaacggtcg cgtccattga aggcttcgcg gatgtcagac aacagcttgg
      241 caccgaggct ctcatcggct gcagcccgac tgccgaaaag ctcgattaag gctttacggg
      301 cgcggctggc ccactcagtc ccggcgagat cggcaacggc cagcagcggc tccgcgacgt
      361 cctgttggcg gtcggacaac gcctcgggca gttcgggaaa agaacgccgc agaatttcta
      421 agcggtcttc ggtcgcccac cctgttaatc ggtctcgaag atcagtggcg tcctgggcga
      481 ccagccgcgg ccggaaccgc tccactgtct cgccggggcg ctttcggaga aattgaaccg
      541 ggatcgcacg gtccgcaacc gtatcgggca gcctacctat accggcgatg acttttgggc
      601 caaacacatc gaagtcgaca accttgatgt cgcaacccct acccacgcag agcgaagccc
      661 gccctccttt tcgatgcccg gcgttcaaca ctccgcgaag cgcctcggag tattccttat
      721 caccgctaaa cgcggcgtcg gactcatcca gcaacaacgt gggatggctg gcagccaact
      781 tgcggacaag tgccgcagcg gaagtcctgg cggtcatcca aggccgcctg accaaaagct
      841 cgagcaattc caggaggcgg gttttccccg atcgcttctc tgctgatgtg atctgtaggt
      901 acggcgcaca ctcaaactgt tcactgctgt acgtgtaaag aacgaacaaa cacaatgtca
      961 cggcctgagc aacggagacc acgaggaaac gttggataaa actccgcaca tcctccagaa
     1021 gggcagccgt ctcgtgctcg tcgttcgtcg gctcgggagc aggcgcgtat cgggagacgg
     1081 accggacaat ttcccgaacc tcggattccg gcaagggcgg ctggcaccta ccagcgtttt
     1141 ccgccagtag tgcggcctcg atggcttttg gagtcatacc ccgccgccgc attgcgccag
     1201 catccgaaag tagcgttgcg ttgcgctggc cttcggcgat gaggccggca ccaactctgg
     1261 ccgccagagt tgcgcttgtc tgcgcgcgcg cccgcaccag ctcaagaagc cattctggag
     1321 ctcgcgcgat gggaacattt tcattctccc aatcgtagcg atgaccgctg gaatggacgc
     1381 tgggcggaac aactgcgtag ccgcccgtgc cgcgaatatc caggcccggt gccagtttgc
     1441 ttgtgctgtt gcgaattgtt ggtccatcgg gatagcagaa gtagaggtgc gccccgcgtg
     1501 aggttttcac tctaagggtt ttcatccagt catggccatg acgctcgctg agtgcgcgaa
     1561 tggctgcttg accctcggtg ccatcgatgt cgagaacgaa gacgcgagag tcgataccgc
     1621 acgccacgcc ccagttgcag ccggggtgcg cctgcatcca ggcgcgcaga ctctcggcgt
     1681 cacacgtcgc cctttccgtc cacttcttga ttagcgcacg cttgcccctt ttttcgacgg
     1741 gaaagaggcg aaagccgcgc gcagccagtc ctagcacggt gatctcgtcc gcggtcgatt
     1801 gcgtccgtcc tcccattagc gacgaaggca agggcctcag atccgacgcg gcggttgcca
     1861 tcagttcgcc tcgcacttcg cagttgccga actaacgccc cggctctgcc ggattcgatc
     1921 ctcaatccac gcttgaatgt cttcctcaag ccatccgact gcccgagcac cgagtgagat
     1981 ggaatttggg aacgtgccct cggcgatttt caggtagatg gaacttctgg gaagcccggt
     2041 ttgtgccttt actaaaggca gtttcaggat tactcttgcc atccctgtac ctcactggat
     2101 ctgacttaca acccagtcat atcagggctt agaaggacaa aagtagggcc ctacttttgt
     2161 ccggttttct tatccggaga cgattagcga tggctctcga ttacagcaag ttggttgaca
     2221 gatccttcaa gtacataggt cccaagaacc ctttaagagg attgagcggt cccgacagaa
     2281 cgctcctgtt ttctttactc ttcatttatc gctttgagca gactatgcct caccgatcaa
     2341 aagcagttga ggccgcagag gagatgtcaa aaatagccgc cgactccacc gcactggcac
     2401 aacgaattcg ctccgtgatg atcgaaggca agttcgcatt gcctttggac ccattccttc
     2461 cagggtttcg gtatctcccg tcgcatctgg acctatttgg taaacgattg ggcacgctga
     2521 tgacacatct ggcgggaaag cagggtcaga aacacaagac gactgcaaat cagcttctca
     2581 tcatggcgtc agaattggtg cggctgaaaa ctggcgagcc ccacgatgaa cacttggctg
     2641 aactacttca ggcgattagt ccagacgccg aacccgggcc tgatacggac atctctggag
     2701 actcaattaa gaagaaaaga cggtacatga gcaagacgta tcccgcgatg tacagctcaa
     2761 ccatcagcag attgcgcagg atggcatcaa gaagttctcg aagcgactag cgggacatcc
     2821 tggcaataga agccggccgg tttggctacg acgcgactcc aaatctggca atcagaactt
     2881 tcttctcgct cgctaatccc tcgacgtaat cggcccagaa ttgcagcatc tctttgcgct
     2941 ggtcagagta ttccgctttg ttatagactc cccgcacccc gccaatcgta tgatttagcg
     3001 acttttctac aacgtcagag gagaaaccct tttcatgcag caaagtggag cctgttcgtc
     3061 gtagatcgtg aattgtgaac ggttccatcg ggaatgtcat gctggccagt gcctggttca
     3121 tcgcattgtg cgcgaatggt tttgcggggc tactgcggct cggcaacacc cactgagact
     3181 cacctgccaa aggcttgagt tcgcgaaaca aatcagcggc ctgagttgat agatagacaa
     3241 cgtgcggctt gcccgttttc gagttcgccg ccggtatttg ccattcgccg ctctccaggt
     3301 ccacatcaac ccacttggca aacaacagct cagacttgcg caccagcgta agcagtataa
     3361 ggtggagcgc aagcttgaat tgcctcctga tgttcgactg atagagggag cgcaagtaca
     3421 cgcggatttc atcgggcgac aacgtgcgcg ttcttgacct agctttcgtg ataaatcgca
     3481 tcggcaatgc cagcgcggga ttcgcctgtg ccgctccgcg cactatcgcg tagtcgaaca
     3541 accgcttgat gagattgcgt atggcggctg ccgcggcctc cagcccgttg tcccgtttac
     3601 ggaaaaccag tgtttgcaca tcggcggctg tcacgtcctt gagcgctttg ttgccaaaag
     3661 ccgggaaaat ctctttgtcg aggtagcgac ggacgctgct tgggtccttc aatgacctaa
     3721 gcaccacgtc agtgtaatag cgctctgcaa actcgcgcac gctactgtcg gtcccaagag
     3781 ccagcttcgc aagctgtttc tcccgcgccg gcgactggcc tcgcgccacc tgcccggcca
     3841 tcacgtcccg cttgtccctc gcatccttga gactcacgtc tggatatcgt ccgagcgcta
     3901 ccttctcgag tttgccgtgc aagcggtagc ggtagcgcca agccattgct ccactgggaa
     3961 ggacttccac gcttaggccg cgtccgtccg tgaccaagta acgcttcgcc ttgggcttca
     4021 gcgctttgat tctcatatcc gtaagtgcca tacgaacccc tctcgtgtgt ccaccacagc
     4081 caaatttggg attctgtccc catggcggac acgccggtgg acataaaatc tgtcggcttt
     4141 ctgtgtctac cacgggacag tataggaaca taatcctatg atagcaagga gattgtagag
     4201 ggttattgga ttccacggga cgctagcgga tatgatttta ctttccaatg cagaatgagc
     4261 tgaagatcag gttcagaatg tcgtcggcgg tggtggcgcc ggtgatgtcg tccaagggac
     4321 gcagcgcgcc gtagaggtcc agcagcagca tctcgtgcgg tattttcaac gggaccgccg
     4381 cgtttgcttg gtcgagcgca gcaattgcgt cctcgaccag cttctgttgg cgcacgttgg
     4441 tgagaaagcc gctctcctgc tgcgcgccgg aatcgccgcc cagcagccgc agaattgccg
     4501 tgcgcaactc cgcgatgcct tggccagtga tcgccgaagt ctggacaaca ggcagcgtga
     4561 gcgcggcggg aagaggcgcg gtggcatttg cgacatcaat tttgttttga gcgaccacgg
     4621 ccaggcgctg cgaagccagc gcgatcagtt cggcgtcgcg ctcgcgcgct tcgtcatcgg
     4681 cagcaggatg cgatgcatcc agcaccacca gtaccatgtc cgcctcagcc agggcctcca
     4741 tggatttgcg cacgccaatg ctctcggctt catcgagcgc tgggcgaatg ccggctgtgt
     4801 ccaccagccg caccggaatg cctccgatgg caaccgtttc gctcaccagg tcgcgcgtgg
     4861 tgccgggcgt cgcagtgacg atggcgcgtt cacgctccac caagcgattg aacaagctcg
     4921 acttgccgac attgggacgc cccacgatag ccaaggtcag tccttcgtgc acaacctttc
     4981 caaacgcgaa gctagccagc agctccgcca atggcgcgcg aatcgcggcg acgcgtgaga
     5041 ggacctgcgc atcgggaatc accgcgacat cgtcctcggc gaaatccacg cccgcttcca
     5101 tgatggcaat cagctcaatc agcttctgct tcgccggctg taatcgcttc gacaaggcgc
     5161 cttcgagttg ccgggccgct acctgcgcct gatacagcgt ttgcgactcg atcaaatcgc
     5221 gtaccgcttc ggcctgcgtc aggtcaatgc gcccgttgag aaaggcgcgc atggtgaact
     5281 cgccgggttc ggccagccgc gcaccctgcg ccatcgccat ctgcacaaca tgccgcagaa
     5341 ccaccggcga gccgtggcaa gagatttcga ctacatcgtc ggtggtatac gagtgcggct
     5401 tggcgaagaa cgtaacgacg acttcgtcga tgcgctcgcc ggtagaaggc tcgatcagtt
     5461 cagcgaaatg agcgcggccc ggctcgaaat cgtgtccatg ggccagtcgc agcatgggac
     5521 gcgcgatggc gcgcgcctcg gcgccagaga ggcgcacaat gccgatgccg ccgcgtcccg
     5581 ggggcgaagc agtggcaacg atggtgtcgt caagatgcat ggaacgattg tagcgggccc
     5641 gaaagaagac ggttcaccac ggagacacgg agaacacgga ggaaattaca agctagcgaa
     5701 agaactaatc cagtcggtcg taccgtttcc agccgtccgg caggttggac gcgcggcaaa
     5761 tgaacagtgg aattttgtcc gccgcgcaga tagacattac ttgctcgaga aacgtatttt
     5821 ctgactttga gtacgcttca agattcgttg ttacaatcca cactccattc tgcttgacag
     5881 agctgctttg cttcctgaca aaggaacgaa attctgctcc ggtctgaaga gttgtggatc
     5941 cggcgtcggg agtgagatag tcggccgcgc ttacggtcag tccccacttg ctgccggaca
     6001 aatcagccac acgcttgggc aacaggtgca tggaaatacc ctttgagcgc tccggcgtgg
     6061 ccccgacggc tagcgccgta agcaacagaa tcgagacggc ggtgagtttt gttttgttca
     6121 acttcttgat tctccgtgcc ctccgtgtct ccgtggtgaa cccgccttat cttcgacggg
     6181 aaagcggcgg cggcgcgggt ggtttgcctt ggtagtcctt gggatagacc acaacgtgcc
     6241 gctgaagggc ttcgccatcg ctgtcggtgc gcaggtcgtc gaaatcgcgc agggccagat
     6301 gaacgatgcg gcgctcccgc gacgacatcg gcgcgaaggc atacggcaag ccgctcttgc
     6361 gaacccgctc agccgctacg tccgcagcca gtctaagctc ctgtaggcgg gcggagcgga
     6421 aattctggca gtcgaagctg accttctcgt gttcgttggg agcaagacga agcatctcga
     6481 gcgccacatt ctcaaaggcg cgcaacaact ccgcgccgtt ctccagcacc agcggactgt
     6541 cggcgccgga aatctccacc aggatttcgg ggcgctccca gtcgcggtcg ctgccgagct
     6601 gaggatcgac ggtgatcttg tagcgcagac ggaagtcgcc gttggcgaag gtcgtggtca
     6661 gcagttcgct gatcttcttg gcggccgcga ttttgtcatg tatcggcata agaagttact
     6721 gggactgaag tccctttacg atctgtcgtc cctgaaggga ctcggatcta cttactacgc
     6781 ttacccagcg ctgaagcgct gggctatcat ttttcgtccc gccttgcggg actagagtct
     6841 cgccacatca taatctggcg ggcccgttga tattcaaata agccaggcgc agcacatggt
     6901 agtcgctctt agaaacgaat tccgctactt actcgccttc ttgcgggcgc gcttttccat
     6961 ctcttcgcgc atctcccggc ccaggccggt acggttcatg atgtactgct gagcgatcgc
     7021 aatgaagttt cccaccgtcc agtacacgct caagcccgca ggcagggaat agctgaagaa
     7081 gccgatcatc agcggcatca tcaacgtcat catctgctgt tgtttcggat ccatgccggc
     7141 gttgggggtc atcttttgca ccaggaaggt cgtgatcacg atcaagaccg gcagaataaa
     7201 tatcttgtcg ggtgacgaca gatcgtgcag ccagaggaag ggcgcctgcc gcagttcgat
     7261 cccgactccc agcatggaat agaacgcgaa caggaacggc atctggataa tcaggggcac
     7321 gcatccgccc gcgggattgg cgccttcgcg tttgtacaac gcaccgatct cgacgttcat
     7381 ctccgagcgc cgcggatcgc gcaatgggta cttcttgtac ttttcctgga tggccttcat
     7441 ctggggctgc agcttttgca tcttcagcgc cgacttcatg ctggtgatgc gcaacggcag
     7501 cagcaccagg ttgatgatga cggtcaggat catgatgcac acaccccaat tgctggccat
     7561 gtgctcgtgg gtccaaagca gccacaggaa aagcgggcgg gcgatcaggc tgaagaagcc
     7621 gaagtccacc acgttgcgga ggtcgggacc gttgatttgc cccggcgcgg tgctggaacg
     7681 taccgactcc agaacatgga gcgctttcgg cccaacaaac aaccgctctc gcgtaatgcc
     7741 gctgacatcg cctacggcag cgcccagcgc atcgtaatgg tttaagttgt tgggatccgg
     7801 attcttcaga tctttaggta cgctgatcga attgtgcagc gtgaccatgg ctgcggagtc
     7861 gggattgtcc ggcaaaaaga tggccgcgaa atactggtcc tgggacccgg cccagttaaa
     7921 gggtccgcgc aaggtgttgc cgctgctcac cttcttggga gccaggcgct ccaccttgtc
     7981 gttagccatg tactcgatct tggccgaagc gtaggacggt ccggtgctct gatcgccaaa
     8041 ggccgccggc cacatgggat acgcctgcac aggcttgcca ttctgcgtga ctgtcgtttc
     8101 tatcgagacc acgtacgtgt cgtcgaactg gaaggtcttg cggaccgtag tatcgccttg
     8161 cgcgtactcg taggtcagct cgcccggggc cgtgatggcg ccgctggcgc tgcctacgta
     8221 gagcgccgaa ttgacttggt tccgcaggtc ttcgtcgtac gcgtagaggc tgagtggaag
     8281 cccgaacttc acggccagcg ggttcaccag gtcgagcgga tgtccgtctt cgtccttgta
     8341 tttcttcagc acccacgact tcgcctgcgc gccacggttg gtaaaaacga tgcggaacac
     8401 gtcgttctcg acgacggttt cgacttcgct gggggcagat ttcgtaactg cggatttccc
     8461 ggtcggcttg gtgacggctg gagcccgacc cttggagaca gcctccaccg gtgcgcccgc
     8521 ggcttcggac tgggactggg gcgcgttaga ctgcgaggtt tggtcgggag ccgactttgg
     8581 ctgcggcttg gacttgtctt tgaagagaaa gatctggctg atgatgatca gcgcaaaggt
     8641 gacggcgaac accatcaaca tgcggcgatc gccgccgggc tcttgctgtg gatttttata
     8701 ctcggccaag tttaagttcc gttcaagggg tgagaattcc ccagcgaatt atccggagat
     8761 ccgtaccctg gtttcacgat cgctggcggc accggatcga atcccgcgcg cgcgaaagga
     8821 tggcagcgca gcagccgcgc ggccgccagc aggcttccgc gaatgacgcc gtgggcctcg
     8881 acggcctcga tcgcatattg cgagcaggtg ggaacgaagc ggcaggcatg cggcagcatg
     8941 ggcgagatga ctcgctggta gcaccgcagc acgaattgca ccgcctgctt catttttgtt
     9001 cccctgtccg ggcgcgttgt gcggccagcc gcagggcgcg cgtcacttcg tttgccaatt
     9061 ccgcgaaggg catgttgcta acgctctttc gcggattaaa caccacgtca atggggccct
     9121 cgcaggcctg ggccgacagc cgtaccgctt cgcgcatccg ccgcttgatg cggttgcgct
     9181 cgactgctcc tcccagcacc ttgcccacgg tgaatcctac acggggcgtg ttgcccgccc
     9241 cagtcgcttg cgggccgcta ccacgccgca gaaagaacac cgtcatgttt cccgtgaact
     9301 gccggcgacc gctctggtac acccgctgaa aatccgcg
//
LOCUS       DASRKK010000392         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_811823, whole genome shotgun sequence.
ACCESSION   DASRKK010000392 DASRKK010000000
VERSION     DASRKK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811823"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFC15_08895"
     CDS             complement(<1..580)
                     /locus_tag="VFC15_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM10313.1"
                     /translation="MKSAVASSKAERRKQGKALREKCPRTAQAEWKPRSKSQDIIKLL
                     EESDADRIAGLIPVKYQRMSVSPFTFFRGTAIIQARDLASAPVSGISVQACGDCHLAN
                     FGGFASPERVLVFDINDFDETFPGPWEWDVKRLGASLILAARDRGFSKTVADRAVRAA
                     AASYREHMAEFAEMKVLDTWYAQVSIDAIRDYF"
     gene            complement(653..1843)
                     /locus_tag="VFC15_08900"
     CDS             complement(653..1843)
                     /locus_tag="VFC15_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10314.1"
                     /translation="MTIPSLTEEVPDLSLVLGGPIFQLFRRSHLSGDALELLHRRIVA
                     IVLVAWLPLLLLSILGGHLLAGTVRLPFLHDIEAHVRLLVALPVLIAAELIVHSRIRP
                     VVKAFLARRIVRQQDVPRFLAAIDSATRLRNLVPVELGLVILVFTVGQWLWRSQIALG
                     ASTWYAIPQAGHMHLTTAGYWYEIVSIPIFQFVLLRWYLRFFIWFRFLWQVSKLDLHL
                     IPTHPDRAGGLAFLGKSSYAFSPILFAQGALLAGVIASRVLYGGESLLSFKLEIVGFI
                     GLFLVLILAPLAMFTPQMARAKRKGLAEYGLLANRYVDGFEQKWVVGNAVDGDELLGT
                     RDIQSLADLGNSYAAVRDMRAVPFGLEDMGRLAAATAVPLLPLGLTVFSLEELVMRIV
                     KIIF"
     gene            complement(1887..2759)
                     /locus_tag="VFC15_08905"
     CDS             complement(1887..2759)
                     /locus_tag="VFC15_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10315.1"
                     /translation="MFKQVLRAILFLLLCSVVLSGDRAFAQDPQGKPVPSDEAKPAAA
                     GDTEALAKAAQNPVASLISVPLQNNTAFGVGPYDRTQNVLNIQPVIPISISENWNLIA
                     RIIQPIVWQPNVSQTSQGWFGLGDMNPTFFFSPAKPSKLIWGVGPTFVLPTATADQLG
                     QGKFSMGPGVVLLTTPGKWVIGTLINNVWSVAGSGSRPPVNQMLLQWFVNYNMKKGWY
                     LASSPIVTANWRASSGNVLTLPFGGGVGRIMKLGNQPVNILAQLYGNAVHPEGAAPWS
                     MRLQIQFLYPKKPK"
     gene            complement(2834..3247)
                     /locus_tag="VFC15_08910"
     CDS             complement(2834..3247)
                     /locus_tag="VFC15_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HZM10316.1"
                     /translation="MSNESREDAEQSVDPRVDLAVLRSELAWDRTLLAWVRTVIGLIG
                     AGVGFDKLAQYLHQARLEAGTAIVRNGHLVGLTLTGVSTVMLMIACLQYWQSRRELAR
                     IKGSRPPRIPPAMLASILVIVLGCAVFVVLLGDKP"
     gene            complement(3334..>3672)
                     /locus_tag="VFC15_08915"
     CDS             complement(3334..>3672)
                     /locus_tag="VFC15_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10317.1"
                     /translation="SKDKLTPGPHTIVYDFKYDGPGLGKGGVGTIQVDGKTVATGRIE
                     RTLAFRLSLDETLDCGEDTGTPITEDYQVPFKFTGEIKKVVIDLTPEQLSAEDKAKMK
                     EAEAAIATAD"
BASE COUNT          814 a         1094 c         1006 g          758 t
ORIGIN      
        1 ggaaataatc ccttatggcg tcgatgctga cttgcgcgta ccaggtgtcg agcactttca
       61 tttcggcgaa ttctgccatg tgctcccgat aagacgccgc agccgctcgc accgcccgat
      121 cagcgacagt cttggagaaa ccccggtccc tggctgccaa tatgagactg gcccccagtc
      181 gtttcacgtc ccattcccat ggaccgggga aggtctcgtc gaagtcattg atgtcgaaaa
      241 ccaacactcg ttcgggggat gcgaagccgc caaagttcgc caggtggcaa tcgccgcagg
      301 cctgaacgga aatccccgac acaggcgcac tggcgagatc ccgtgcctgg atgatggccg
      361 tgccgcggaa gaatgtgaaa ggcgacacgg acattctctg atacttgacc gggattagac
      421 ccgcaattcg atcggcgtcc gattcctcaa gtagtttgat tatgtcttgt gacttcgagc
      481 gaggcttcca ctcggcctgg gcggtacgcg ggcatttctc gcgaagcgct tttccttgct
      541 tgcgtctttc agccttggat gaagcgacag cagatttcat tcttattctc cttgagcgcg
      601 tggtcccgac tgctacctcg gcgccggcgt taatcgtgta tatttcgttc cgtcagaaga
      661 tgatcttaac gatccgcatc accagctctt cgagggagaa cacggtaagg ccaaggggca
      721 gaagcggcac cgctgtagcc gcagcgagcc ggcccatgtc ttcgagaccg aagggcactg
      781 ctcgcatgtc acgcacggca gcataactgt tccctaaatc ggccagcgac tgaatgtcac
      841 gcgtgcccag aagctcatcg ccgtcgaccg cgtttcccac cacccatttc tgctcaaatc
      901 catcgacata gcgattcgcc agcagcccgt actccgccaa tcccttccgc ttcgcgcgcg
      961 ccatttgcgg cgtaaacatg gcgagtggcg caaggatcaa tactaagaag aggccaataa
     1021 agccgacgat ctccagcttg aacgacaaca agctttcgcc cccgtacaag acgcgactgg
     1081 ctatcacgcc tgccagcaac gcaccttggg cgaacagaat tggactgaaa gcgtaggagc
     1141 tcttccccag gaaagccaaa cctcccgctc gatccggatg ggtgggaatt aggtgcaggt
     1201 ccaatttaga aacctgccac aggaaccgga accagatgaa aaaccgcaga taccagcgca
     1261 gcagaacgaa ctgaaaaatt ggaatgctaa cgatctcgta ccaatagccc gcggtcgtca
     1321 ggtgcatgtg accagcctgt ggaatcgcat accaggtcga agcacccaga gcgatttggc
     1381 ttctccatag ccactgtccg acggtgaaga ctaagatcac aagtccaagc tccaccggaa
     1441 ccaagttgcg caaacgtgtc gcagagtcga tggcagcgag gaatctcggc acgtcctgct
     1501 gccggacgat gcggcgcgcc agaaaagctt tcactaccgg acgaatgcgt gaatgcacaa
     1561 tcagttcggc agcgatcagc acgggcaatg ccaccagcaa tcgaacgtgc gcctcgatgt
     1621 cgtgcaggaa aggcagcctc accgtgccag ccagcaagtg tccgccgagt atcgacaaca
     1681 gcagcaaggg cagccatgcg accagcacga tcgccacgat ccgccgatgc agtagctcca
     1741 gcgcatctcc agaaagatgg gatcgccgga acagctggaa gataggtccg cccaggacca
     1801 gcgacaaatc cggcacttct tcagtcagtg atggaatggt catggttcta gtcataggtt
     1861 aaccttggaa ccggcgaatc cagccattac ttaggcttct tgggatacaa gaactgaatc
     1921 tgcagccgca tgctccacgg ggcagctccc tcagggtgca ctgcattgcc gtacagttga
     1981 gccaggatgt taactggctg gtttcccagc ttcatgattc ttcccacgcc gccaccgaag
     2041 ggcagcgtca atacgttgcc gctgcttgcc cgccagttgg cggtgacaat cggcgacgag
     2101 gcgagatacc agcccttctt catgttgtag ttgacgaacc attgcagcag catctggttg
     2161 actgggggac ggctccccga acccgcaacc gaccagacgt tattgatcaa ggtgccaatg
     2221 acccatttcc ccggcgtggt caacaaaacg actcccggcc ccatgctgaa ctttccctgg
     2281 ccaagctgat cggcggtagc cgtggggaga acgaaggttg gcccgacccc ccaaatcaat
     2341 ttgcttggct tcgctggcga gaagaagaac gtgggattca tgtcgccgag tccaaaccag
     2401 ccttgagaag tctgactgac gttcggctgc cagacgattg gctgaatgat gcgagcaatc
     2461 aggttccagt tctcgctgat gctaatcgga atgacgggct ggatattcaa tacgttttga
     2521 gtgcggtcat acggtccaac tccaaaagcc gtgttgttct ggagcggaac gctgataagt
     2581 gaggccaccg gattctgcgc ggccttcgcc agcgcttcgg tgtccccggc tgccgcaggt
     2641 ttcgcctcgt cagacggaac aggtttgcct tgtggatcct gcgcgaaggc tcggtctccc
     2701 gacagaacga cagagcagag cagtaagaac aggattgcgc gtaacacttg cttgaacatt
     2761 gttagtctct ttctgctcgg tttggagggt tcctcgtgtt tggaaacctc acgtttagaa
     2821 ggaactgcgt ctctcagggc ttatcgccga ggagcaccac aaagaccgca cagcccaaca
     2881 caattacgag aatggaagcc aacatcgctg gtggaattcg tggaggtcgc gagcctttga
     2941 tccgggccag ctcacgcctg ctttgccagt actgcaaaca agcaatcatg agcataaccg
     3001 tgctgacgcc ggtcagagtc agcccaacca gatgcccatt ccgcactatc gcagtgccgg
     3061 cttccagtct cgcctgatgc aagtactggg ccaatttgtc gaagccaacg ccagcgccaa
     3121 tcagtccgat tacggttcgc acccacgcca gcaacgtacg gtcccaagcc agctcagagc
     3181 gcaacacagc cagatcgacg cgcgggtcaa ccgattgttc ggcatcttca cgagactcgt
     3241 tggacatctc tgcttcctct tgttgttcac ttcaccaact ggatgtcgcc gggctcccaa
     3301 cgcttgcaac gccggaggca gttctgtcac caatcagtct gctgtggcga tggcggcttc
     3361 ggcttctttc atctttgcct tgtcctccgc gctcaattgc tccggcgtca ggtcaatcac
     3421 aaccttcttg atctcgccgg tgaacttgaa cgggacttgg taatcctcgg tgatgggtgt
     3481 tccagtgtct tcgccgcaat ccagcgtctc gtcgagcgag aggcggaacg ccagtgtgcg
     3541 ttcgatgcgg cccgtggcca cggtcttgcc gtccacttgg atcgtgccga cgccgccctt
     3601 gccgagacct gggccgtcat atttgaagtc gtaaacgatg gtgtgcgggc ccggcgtgag
     3661 tttgtccttg ct
//
LOCUS       DASRKK010000393         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_127381, whole genome shotgun sequence.
ACCESSION   DASRKK010000393 DASRKK010000000
VERSION     DASRKK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127381"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFC15_08920"
     CDS             complement(<1..254)
                     /locus_tag="VFC15_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZM10318.1"
                     /translation="MAPLRISAISFLNTAPLMWDFEHGDAARDFAIEYTIPSACAAAL
                     AANQADIGIIPAFTYAEIPGLVILPNIAIAAKDFVRSILLV"
     gene            410..1180
                     /locus_tag="VFC15_08925"
     CDS             410..1180
                     /locus_tag="VFC15_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HZM10319.1"
                     /translation="MDTPMGAVIVIGTSMLLSIAGLLVVRRYANVDWLKRHHEIASYF
                     FLMIGTLYAVLIAFAIYVVWSASKEAGTNLEHEATEVADLSRLSMAMPDPLRRKITTA
                     LMEYLNAVVKDEFPGMEQGRDSQRTWTAVQNLWDVYGAIQPETPRLQIYFAESLKHLT
                     QLSDYRRTRLLASRGTVPSTLWYLLLSGAVLLVLFTYFVGHESAWAQAAMTAALAGVL
                     AFSLFLVWSLDSPYSGVASVTPQPFSLELVHVASRMPK"
     gene            1491..>2086
                     /locus_tag="VFC15_08930"
     CDS             1491..>2086
                     /locus_tag="VFC15_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZM10320.1"
                     /translation="MHAEAPLRAHDYAVTRKLQWLALSLTPGVGAGRGRKLVEMYGGI
                     ERLFTASLTELEAAGLPIAAAQSLALGKSLELANAEFDHARELGAAVIVPGDAEFPRR
                     LLEIYDPPLVLYLRGSAEIIDKPGVAVVGTRHPTPYGTGMAERLACDLAARGLIIFSG
                     MARGVDTAAHRGALQAHGHTVAIWGTGIDVVYPAENRK"
BASE COUNT          423 a          594 c          627 g          442 t
ORIGIN      
        1 accagcagga tcgatcgcac aaagtccttg gccgcaatcg caatgttcgg caggatcacc
       61 agccccggta tctctgcata ggtgaacgcc gggatgatgc cgatgtcagc ttggttcgcc
      121 gccagggcgg ccgcgcatgc ggagggaatg gtgtattcga tggcaaaatc gcgggccgcg
      181 tctccgtgct cgaagtccca catcagtggc gcggtattca gaaaggaaat agcggagatg
      241 cgcagcggag ccataaccaa tcgagtctag caaagctagc gtggtcgcgt cggtgccgtg
      301 agtcgcttaa ttgggtatta atctgcgcgg cggcgcaatc acgtcgatgt gtgacagctg
      361 gtctgttcat tgcccattcc aggccccatc attacgacag gagactggga tggacactcc
      421 gatgggcgct gtgattgtca ttggcacctc aatgctgctg tctatcgcag gattgctagt
      481 ggtgcgtcgc tacgccaacg tcgactggct gaagcggcat cacgaaattg ccagctattt
      541 cttcctgatg attggaacgc tctatgccgt gctgatcgcc tttgccatct acgtagtctg
      601 gagcgcgtcc aaggaggcgg gcaccaacct ggaacacgaa gcgaccgagg tagccgacct
      661 gtctcgtctg tcgatggcaa tgcctgaccc actgcgcaga aagatcacca cagccctcat
      721 ggagtatctc aacgccgtag tgaaagatga gtttccgggt atggagcaag ggcgcgacag
      781 ccagcgcacc tggaccgcgg tgcaaaacct gtgggatgtg tatggggcga tccagcccga
      841 aacaccacgg ttgcaaatct acttcgcgga gtcgctgaag cacttgactc aactctcgga
      901 ttatcgccgg acccggctgt tagccagccg tggcacggtc ccctccacgc tatggtacct
      961 gttgctttct ggcgcggtgc tgctcgttct cttcacctat tttgtcgggc acgagagcgc
     1021 ctgggcacag gccgccatga cggcggcgct ggcgggggtt ctggccttca gcctgtttct
     1081 agtttggtcg ctggatagtc cctattcggg ggtggcaagc gttacgccgc aaccattcag
     1141 tctggaactg gtgcatgtag cctcgaggat gccaaagtaa tagcacctct cccataggat
     1201 ccagcgccca ttgatccccg gttttaagat caacacgacc ctcacatgca caggtgacag
     1261 actcccttga cggtaaccac ccaactggtt actgtccaac tgtgcaaaga cattcgccgc
     1321 tccgtcgaaa atcaattcac cacccttcgg cttcgctgag gacaggctcc gacacagagg
     1381 gcacgaagtt ttggttctta tcgagtcttc tccgtgatct ccgtgcctcc gtggttgatt
     1441 ttgattccta cggtgggcgc aggcccatgt cgcattcagg ggaaattgat atgcacgctg
     1501 aagcgccgtt aagagctcac gattacgcag taacccgcaa actgcaatgg ctggctttgt
     1561 cgctgacacc gggcgtgggc gccggacgcg gccgcaagtt ggtggagatg tacgggggca
     1621 tcgagcgcct gttcaccgcg tcgctgaccg aactcgaagc ggccgggctg cccatagcgg
     1681 cggcgcagag tctggcgtta ggcaagtcgt tggaactggc caatgccgaa tttgaccatg
     1741 ccagggaact gggcgctgcc gtcatcgttc cgggggacgc ggaatttccc cggcggctgc
     1801 tggaaattta tgatccgccg ctcgtgcttt accttagagg aagtgcagaa attatagata
     1861 aaccaggggt tgctgtggta ggcacgaggc atccaacccc ctacggaacg ggcatggcgg
     1921 agcgcctcgc ctgcgacctt gcggcccgtg gactcatcat attcagtggg atggcgcgag
     1981 gtgtggatac cgccgcccat cgtggggcct tgcaagccca tggtcacacc gtcgcgatct
     2041 ggggaaccgg aatcgacgtg gtttaccccg cggagaatcg caaaat
//
LOCUS       DASRKK010000394         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1300383, whole genome shotgun sequence.
ACCESSION   DASRKK010000394 DASRKK010000000
VERSION     DASRKK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300383"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFC15_08935"
     CDS             complement(<1..228)
                     /locus_tag="VFC15_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZM10321.1"
                     /translation="MALEEYKRKRDFKQTPEPPAKVERGERRRFMVQKHRATSLHYDF
                     RLEMEGVLKSWAVPKGPSLDPADKRLAMQVED"
     gene            337..411
                     /locus_tag="VFC15_08940"
     tRNA            337..411
                     /locus_tag="VFC15_08940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:369..371,aa:Val,seq:cac)
     gene            complement(497..1597)
                     /locus_tag="VFC15_08945"
     CDS             complement(497..1597)
                     /locus_tag="VFC15_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10322.1"
                     /translation="MVENAVRLGLVADIGDDETSAWLKVGELGLVEKYINNPLSGKPT
                     FGEFCAAYVKDGLPFRKKDGRRKTKGTIETYEYHINNHILPRWKDAVAEEMKPLAIRN
                     WLVDLHDGEDYVWETCSKIAGIMSLVFSFVDHNEICSIRNPLDKVTIPASEEDQDEVK
                     VLLPEQVVALLERLPYPVKIAVLLVASTGVRISECLGLTWKHVEWANNRIFIHQTFRR
                     GEMQNRTKTKASNAPVPMCTALAAFLTDWRQQTPYDKDEDFIFASPKLKGKQPLWGQT
                     MNADFVKPAVVALGLVAKGESFGWHRFRHSLSTWANETTKDITVSQTLLRHSKPEMTA
                     VYTHGNFPKALDAQRQYMEQLLAAKLASEAAQ"
     gene            complement(2380..>2948)
                     /locus_tag="VFC15_08950"
     CDS             complement(2380..>2948)
                     /locus_tag="VFC15_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10323.1"
                     /translation="TALPSRLEVKLPPDFQLQIESAKTAYHRFGQYSRALDQIRLCLE
                     HRVVERAELQRMCSELHVPGDFDVAQISWRPDYDPFFYRQLSRRARRIYLFREEYIFD
                     VEKAVVVETPQLGHATYVFAKPRSMDTFLALYTKITKDDIRRNRNNAAERLGFLGRVI
                     HGTNPRAWLKEARQRIGEGIDLAAAMAE"
BASE COUNT          604 a          859 c          812 g          673 t
ORIGIN      
        1 atcttccacc tgcatggcca gccgcttgtc ggcaggatcg agcgacggcc ctttgggaac
       61 ggcccacgat ttcagcacgc cttccatttc gagccggaaa tcatagtgca ggctggtggc
      121 gcggtgcttc tggaccatga agcggcggcg ctcgccgcgt tcaaccttcg cgggaggttc
      181 gggtgtctgc ttgaaatcgc gtttccgctt gtactcctcg agtgccataa gccacttaga
      241 tgaacatatt caagaggagt gctgcaatgt ttttggcaaa gcgccgggcg atgggcgtcc
      301 cagcttgatt tgctacaatc gacataccgc aactgtgggc gcttaactca gcggtagagt
      361 gccatcttca cacggtggaa gtcacaggtt cgaatcctgt agcgcccacc aacatatctg
      421 cttaccgtcc agccacttac gagtggctgt tgttttttgc cgtcaaaaac ccagcccgac
      481 gccaacccga tggaggtcac tgagcggcct ccgacgccaa cttggctgcc agcaactgct
      541 ccatgtactg ccgctgggca tccaacgcct tggggaaatt cccgtgggtg tagacggcgg
      601 tcatctcggg cttgctgtga cgaagtaggg tttgggagac cgtgatgtcc ttggtggtct
      661 cgttcgccca cgtgctcaag ctgtggcgaa accggtgcca accaaaactc tcacccttgg
      721 ccaccaaccc cagagccacg acggccggct taacgaagtc ggcgttcatg gtctgacccc
      781 acagcggctg cttgcccttc agctttggcg aggcaaaaat gaagtcctcg tccttgtcgt
      841 acggggtttg ctgcctccaa tcggtgagga acgctgcgag ggccgtacac atcgggacgg
      901 gtgcgttgct ggccttggtc ttcgtccgat tctgcatctc gccccgcctg aacgtctggt
      961 gaatgaagat gcgattgttc gcccactcca cgtgcttcca cgtcaagcca agacactcgg
     1021 agatgcggac gcccgtggaa gcgaccaaaa gcacggcaat cttcaccggg taggggagtc
     1081 gctccagcag agcgacgacc tgctcgggca agagcacctt cacttcatct tgatcctcct
     1141 cgctggccgg aatggtgacc ttatccagag gattgcggat ggagcaaatc tcgttgtggt
     1201 caacgaagct gaagaccaac gacatgattc ccgcaatctt tgagcaggtt tcccacacat
     1261 agtcttcgcc gtcgtggagg tcaacgagcc agttgcggat ggcgaggggc ttcatctcct
     1321 cggcgacggc gtctttccag cgagggagga tatggttgtt tatgtggtac tcatacgttt
     1381 cgatggtgcc tttcgtcttg cggcgaccgt ccttcttgcg gaatggcaag ccgtccttga
     1441 cgtaggcggc acaaaattcg ccaaaagtcg gcttaccaga gagggggttg ttgatgtact
     1501 tttcgaccag gccaagctca ccaactttga gccaagcgga cgtttcatca tctccgatgt
     1561 cagcgaccag gccgagcctg acggcgtttt ctaccatctc accgtccctc tctctctgcc
     1621 tttggtaaca ccagagccaa atcatcccat ccgcccgttc acgctgcctg agccagccgc
     1681 gtgctttaac ttccttgtta gccattggat ttgctcctcc ggctcgtcgg ggctgctgcc
     1741 gagactgtac tctgggcagg cttcgtcaag gccgttatgt cttcagccac ctcactcagc
     1801 cgaaagcgcc actgattacg aatgctacca attggatacg cacgaataac ccgcctccgt
     1861 gcaagctcca tgaccttgcg gatgggcagg gacaaatact gggcaacaac ctcctttggt
     1921 acgaactgtt cgttattcat cgtcatggtg actcacttcc gcagctgtgt agccccagag
     1981 agttggtcgc tgagagccag ctttagtcag tttgttgcgt gtcgtatggc ttcttttcga
     2041 tgcccgagcg ccagacgcaa cagtggcctt tcaaccgctg ttgttagcct caaatcgact
     2101 cttcgtcaaa cggcctcatc atgtgagcag acccggagcc cgggtgttat ggactgatct
     2161 gctggtgact gtttaagagg tagcaagaag acctcgctca aactgttcgg caacaatgtc
     2221 cttcgtggcc tattggtcga accaccgacg cgcgatggag ccgcaattct cggctaagaa
     2281 tatttcaccg attacacttt gactgggcaa agcagcaggt acgcagaaat cctccgctac
     2341 atcgaatgcc acaagagtgg gcgcgaattg ttccatgatt cactccgcca tggccgctgc
     2401 aaggtcaatt ccttccccga tacgttgcct tgcttccttg agccacgctc gtggattggt
     2461 gccatgaatc acgcgcccca gaaagcccag ccgctcggcc gcattgttgc ggttccgccg
     2521 gatgtcgtcc ttcgtgatct tggtgtacag agcgagaaag gtatccatgc tccggggctt
     2581 ggcgaagacg taagtcgcgt ggccaagctg aggggtttcg acgacgacgg ctttctcaac
     2641 gtcgaaaatg tattcctccc gaaacagata gatcctgcgc gcgcggcgtg agagctggcg
     2701 atagaagaat ggatcataat cagggcgcca gctgatctgg gcgacatcaa agtcaccggg
     2761 aacatgcagc tcggaacaca ttcgctgcag ctccgctcgc tcgaccaccc gatgttccaa
     2821 gcacagccgg atctgatcca gagcccgcga atactggccg aaacgatgat aggcagtttt
     2881 ggcagattcg atttgcagct ggaaatcagg tggcagtttg acctccaacc ggctcggcaa
     2941 cgcagttt
//
LOCUS       DASRKK010000395         7689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_812150, whole genome shotgun sequence.
ACCESSION   DASRKK010000395 DASRKK010000000
VERSION     DASRKK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812150"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..320
                     /locus_tag="VFC15_08955"
     tRNA            224..320
                     /locus_tag="VFC15_08955"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Sec,seq:tca)
     gene            747..1421
                     /locus_tag="VFC15_08960"
     CDS             747..1421
                     /locus_tag="VFC15_08960"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HZM10324.1"
                     /translation="MFDSIKAASGHDETRRKLLDAAGEVFADVGFQAATVREICSRAG
                     VNLAAVNYHFGDKLGLYTELLKTTVANDGATAEHALRALPPEAALRQFLLVMLRQMHQ
                     ADRPSWHTKVMTHELAHPTPALEAVVEHMIKPKARVLYGIVGRIIGRPPLDPRTRMCA
                     HSVIGQVVHYVHARPVIALLWPELEMTPRTVEEIARHITEFSLEALKGIKRNSKRTAP
                     KARRLK"
     gene            1418..2704
                     /locus_tag="VFC15_08965"
     CDS             1418..2704
                     /locus_tag="VFC15_08965"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZM10325.1"
                     /translation="MTEQQTQELKSSGTPATAPSTPTALANGDAEKTGDRKKRLVRIG
                     VVLALLVAAFILWRVLRGPDIPEGIVALSGRIEGDDSAIASKTSGRILEVKFREGDLV
                     KQGDVIATLDDEQIRARENQAQATVAQAEARVTAAEQQIAVLNQQLLQSRLQATQSKE
                     DAAGRVSQAEADLAAAQSDLAQQESNLKLAEFDREAYTRLAKTGAVSERQGEEAVSKA
                     DAQAAAVAASKRRVEAARGALTLAKSSLANLAIRNSQTAGVESQIAQQNTEVASAQSQ
                     LNQAKAQLVEAQANRQDLTIRAPFEGSIVTRTAEPGEVIAAGTPVVTMLDLTKVYLRG
                     FIPEGQIGKVRLAQQARVFLDSDPNKPLEAYVARIDPQATFTPENTYFRNDRVKQVVG
                     VKLQLKSGAGYAKPGMPADGEVLVQGDSWPRHAKRP"
     gene            2753..4723
                     /locus_tag="VFC15_08970"
     CDS             2753..4723
                     /locus_tag="VFC15_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM10326.1"
                     /translation="MSTALEQTSAESSHHGDGSIAIRIENLWKKYGDLEAVRGVSLEV
                     RQGEIFGLIGPDGAGKTSTFQILAGVMRGTSGVTEIYGKASREARSLVGYLTQAFSLY
                     PDLSVAENIRYVGDLRRVPPEEIKRRSRQYLSMFDMDRFADRLAGRLSGGMKQKLSLV
                     AALVAQPRVLLLDEPTTGVDPVSRREFWDTLAHLADAGLTIVIATPYLDEAERCHRVA
                     LMHQGQIHDTGTPAELRRSMGLKRLEVRTENLGEAELLLTTHTGQQQIFDVQRFGDRL
                     DVMVHDVDRGREAVEGRLHGVNQVDIRVAEPTLENTFVTTLRALGQEIYTPPFPGSHP
                     HRDLRGQKAIDAKHLTKRFGDFTAVKGINIQVEYGEIYGLLGANGAGKTTAIKMLCGL
                     LEPTHGEMMLAGESGSLRSQAVREQIGYMSQKFSLYDDLTIEENLDFFGGVYGVPASE
                     MADKKSWVMSFSGLEGKQRMVTGSLPGGWKQRVAFGAAIMHEPSVLFLDEPTSGVDPL
                     ARRAFWRMINKLADMGTAILVTTHYLEEAEQCNRLGFMVAGELVAEGTPSGIKHQQGG
                     HLIEFVVDQPQRAADLLKQSMDRWRVSLFGYRLHVVTDEDAAAGTRSVTERLRQAGIA
                     VQDVHEEPFSLEDVFIAVVEKARAHGKFAEED"
     gene            4724..5833
                     /locus_tag="VFC15_08975"
     CDS             4724..5833
                     /locus_tag="VFC15_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10327.1"
                     /translation="MFRRIIAQARKELTQIMRDRLALALALVLPLFLLLLLSNAISLT
                     VNDLPLIVQDLDNSAASRSYIDAFRASVTFRVVSWPANRQPESALQAGAARAVLIIPE
                     HFSRDMARGQTANVQILIDATDANTAKLVSGYAGQITRAYVEQNTGAPSVAMVNPQVR
                     LWFNPGRVSQKFYAPGIFVLGISMFPPLLASLAMAKEGDQKTILQVYVSSISAHEFVL
                     GKILAFTVIALAEGLLTMTTLLTVFGLRLTGDPTPLIVATILYAFCVAAFGTMVGAAI
                     PNQAAAIQAVALGGFLLVFLLSGLIFPVENIPVGLRWISNLIWGRYYIVIVRDALLQG
                     GGWPAMWFNVLMIAIIGAVFYVLAWRVLRPMQVKA"
     gene            5830..6999
                     /locus_tag="VFC15_08980"
     CDS             5830..6999
                     /locus_tag="VFC15_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10328.1"
                     /translation="MNSNLSTPSFWQRLRALMQKEFNQIRRDRRLAISLIVPPTLQIL
                     LFGFALDSNVSNLRLGVLDESKTPESRELVATLTESKSFRLGGYFFEDRTLEKAISQG
                     KVDAGVVIPYDFARDLHRGRPSDIQFILNAVNANTAAIGQGYAEGVLANYNRSLASQG
                     IHPQIRIVAADNSRKGSVNLVPAFLFNPGLVTAWFIVTGTFGTLLVLNGSLVASAAMV
                     KEREAGTVEQLLMTPAGTTEIIVAKITPMFVLLLGMVFLALTLIRFVFGVPIRGSLVL
                     VFGGAMLCLLCGIGIGTFIATFTRSAQQAQLLAFFVNPPLATLSGSLSPVEAMPKWLQ
                     PITMLNPIRHFGIITRGAMVKGSTFAELWPNFLALIAFAIVLMGFSIWRFRKQLG"
     gene            7023..>7689
                     /locus_tag="VFC15_08985"
     CDS             7023..>7689
                     /locus_tag="VFC15_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10329.1"
                     /translation="MNQQWIKQIWTAMCFCLLCCGVCFAQQQGGSSTQITLANQVALS
                     ARTAQSGSVTSQQAPVPGTTQSVNTLNSTVLTTGSYSQSVLSGKPLTGPLSLKEAVGR
                     GLDYNLGAVGLSNASMAAHGQMRVARSILLPNLNGNLREAVQQTNLATLGLRSNFIPQ
                     IVGPYNYFDLRATLTQNVADLTALNNYRSAQENLKAAQMAVKDAHDLVVLAVGGAYLQ
                     VIAA"
BASE COUNT         1564 a         2324 c         2324 g         1477 t
ORIGIN      
        1 tgagtccgcg tgcgtcggga aacaccgcca gttggttgcc ctcgagcggc cgtgtggtaa
       61 agacgtcgag ctgaactact tcgaatgtgc gcttcgccat aagaagactc tacggacaat
      121 ttatcaggat tcccgtgcgg atcggtctcc accaccgggg actgtttcga gagacgtttg
      181 ctcccagttg tttacaatga cggcagcagt agtgccggcc tgcggaagcg aagggggtcc
      241 ggtgactctc ccggtcttca aaactggcga ttggcatctt cgatgtcaat ggtgcgttcg
      301 actcgcacac gcttccgcca attgttttca ataatttgtc cggtttctca gctgaccggt
      361 gattcgcggt tttgcgcggc ggttgcatat ttgaaaataa cgcgcagcag gcagaggcag
      421 aaaaggccgc gatctcctga tcgcttgtgc catgttctgt aggaagcacc gagcacgcca
      481 gaaacgtcac ttctctcatc agcgaaagga cacgaatctg attcgtaact caggcccatg
      541 gtattcggca acccccgctc acggtttccc cgccggcatg atgtctggtc ggagaaagca
      601 ttaagcatcg tccggtcaac cagaatactc aggaaggacc gcgcattggt tatgcagcaa
      661 ctccatgcat taacgaagta tccagagaaa ccatgccacc tcttgacaga attaaaacag
      721 ttgttttaac tgttagtttg aaactaatgt ttgattcgat caaggcagcc agcggtcacg
      781 acgagacacg ccgcaagctc ttggatgcgg cgggtgaggt cttcgcggac gtcggcttcc
      841 aggcggccac ggtgcgggaa atttgttcgc gcgcgggggt caacctggcg gcggtcaact
      901 atcacttcgg ggacaagctg ggcctctaca cggaactgct gaaaactacg gtcgcgaacg
      961 acggggccac cgctgaacat gctttgcgcg ccctgccgcc cgaggcggca ctgcgccagt
     1021 tcctgctcgt catgttgcga caaatgcacc aggcggaccg gccgtcgtgg cataccaagg
     1081 tgatgacgca cgagctcgct caccccactc ccgctttgga agccgtggtc gagcacatga
     1141 tcaagccgaa ggcccgggtg ctttacggca tcgtgggacg catcatcggc cgtcctcccc
     1201 tggacccccg gacgcggatg tgcgcccaca gcgtcatcgg acaggtggtg cattacgtgc
     1261 acgcgcggcc ggtcatcgcc ctgctctggc ccgaactcga gatgacccca agaacggttg
     1321 aggagattgc ccgccacatc acggaatttt ccctcgaggc gctgaaaggt atcaagcgca
     1381 attccaaacg caccgctccg aaggcgagga gattgaagtg accgaacaac agactcaaga
     1441 attgaagtcg agtggcacgc ccgcaacggc tccgtctact ccaacggccc ttgcaaatgg
     1501 ggacgcagaa aagaccggcg accgaaaaaa acgattggtc cgcattggag tcgtgctggc
     1561 tctgttggtg gcggcgttca tcctgtggcg ggtactgcgc ggcccagaca ttcccgaggg
     1621 gatagtggca ctgagcggcc gcatcgaagg cgacgactcg gcgatcgcca gcaagaccag
     1681 cggacgcata ctcgaggtga aattccgcga aggcgacctg gtgaagcagg gcgacgtgat
     1741 tgccacgctg gatgacgaac agattcgggc ccgcgagaac caggcccaag cgaccgtggc
     1801 acaggcggag gcgcgggtga ccgcggccga gcagcagatc gccgttctca atcagcaact
     1861 gttgcaaagc cgactgcagg cgacgcaatc caaagaagat gccgccggcc gggtgagcca
     1921 ggcggaagcc gaccttgccg ctgcgcaatc ggacctggcg cagcaggagt ccaacctgaa
     1981 gctcgccgag ttcgaccgcg aggcttatac gcgcctggcc aagacgggag cggtttccga
     2041 gcggcaaggc gaagaagcgg tctcgaaggc cgacgcgcag gcggccgccg tcgccgcttc
     2101 caagcgaagg gtcgaagcgg cgcgcggtgc cttgactctg gccaagtcaa gtctggccaa
     2161 tctcgcgatc cgcaactcgc agaccgccgg agtcgagagc cagattgcgc agcagaatac
     2221 ggaagtggcc agcgcacagt ctcagttgaa tcaggcgaag gcgcagttgg tagaggcaca
     2281 ggccaatcgc caggacctga caattcgcgc gccgttcgaa ggctctatcg tcacccgtac
     2341 cgcagagccg ggagaggtga tcgctgcggg gacgcctgtg gttaccatgc ttgacctaac
     2401 caaagtgtat cttcgcggct ttattcccga gggccagatc ggcaaagtca ggctggcgca
     2461 gcaggcgcgt gtattcctcg actccgatcc caacaagccg ctcgaggctt acgttgcacg
     2521 cattgatcca caggcaacct ttacaccgga aaacacctac ttccgtaacg accgcgtgaa
     2581 acaggtggtg ggcgtgaagc tccagttgaa gagcggcgcg ggctatgcca aaccgggcat
     2641 gcccgccgac ggagaagtat tagtgcaggg cgacagttgg ccgcgtcatg ccaagcggcc
     2701 gtaagactgt caccgaataa caaagaactc gaccagaaat gagccgacga acatgagcac
     2761 cgccctggaa cagacttccg ctgagtcctc acaccatggg gatggttcga ttgccatacg
     2821 aatcgagaat ctgtggaaga agtacggcga cctcgaggcg gtgcgagggg tctcgctgga
     2881 agtacggcag ggggagattt tcgggctgat cgggccggac ggagcaggca agacttcgac
     2941 cttccagatt ctcgcaggcg taatgcgagg aacgtctggg gtgaccgaga tctatggaaa
     3001 ggcctcacga gaggcgcgtt cgctcgtcgg ttacctgacg caagccttca gtctgtatcc
     3061 cgacttgagc gtggccgaga acatccgcta cgtcggcgat ctgcgccgcg ttccgccaga
     3121 ggaaatcaag cgtcgcagcc ggcagtatct ttccatgttc gatatggacc ggttcgccga
     3181 tcgcctggcg ggaaggctga gcggcggcat gaagcagaag ctgtcgctgg tggcggctct
     3241 ggtagcacag ccgcgagtgc tgctactcga tgaacccacc accggcgtcg atccggtttc
     3301 acgccgcgag ttctgggaca cgctggccca cctggctgac gcaggcttga cgattgtgat
     3361 tgcgactccg tatctcgatg aagccgagcg ctgccatcgg gtcgcgctca tgcaccaggg
     3421 acagattcac gacaccggca ctcccgccga gttacgccgc agcatgggtc tgaagcgact
     3481 ggaagtccgc accgaaaacc tgggcgaagc ggaactgttg ctcaccaccc acaccgggca
     3541 gcagcaaatc ttcgacgtgc agcgctttgg cgaccgcctc gatgtgatgg tgcacgatgt
     3601 ggaccgcggc cgggaagcgg tcgaaggccg gttgcacggc gtaaaccagg tcgatatccg
     3661 agtagcagag ccgacgctgg aaaacacgtt cgttaccacc ttgcgcgcgc tgggtcagga
     3721 gatttacaca ccgccttttc cggggagcca tccgcatcgc gacctgcgcg ggcagaaggc
     3781 gatcgatgcg aagcacctaa ccaaacgctt cggcgatttc accgccgtga aagggatcaa
     3841 tatccaggtc gagtacggcg agatttacgg actgctgggc gccaacggcg ccggcaaaac
     3901 aacggcgatc aagatgctct gcggcctgct ggagccgacc cacggcgaga tgatgctggc
     3961 tggggaaagt ggcagcctgc ggtcgcaggc cgtgcgcgag cagattggct acatgtcgca
     4021 gaagttctcg ctctatgacg atctgacgat tgaggaaaac cttgacttct tcggcggcgt
     4081 ctatggcgtt cctgcctcgg aaatggccga caagaaaagt tgggtcatgt cgttctccgg
     4141 cctggaaggc aaacagcgga tggttaccgg cagccttcct ggcggatgga agcagcgtgt
     4201 ggccttcggg gcggcgatta tgcacgagcc cagcgtgctt tttctcgacg agcccacctc
     4261 gggcgtggac ccactggcgc ggcgcgcctt ctggcgaatg atcaacaaac tggcggacat
     4321 gggaaccgcg attctagtaa ccacccacta cctcgaagag gccgagcagt gcaaccgcct
     4381 cggattcatg gtggccggag aactggtcgc cgagggtacg ccgagcggaa tcaagcacca
     4441 gcagggcgga cacctgatcg agttcgtcgt cgatcagccg cagcgcgccg ccgacctgct
     4501 caaacaatcc atggaccgct ggcgcgtgtc cctcttcgga taccgcctgc acgtggtgac
     4561 cgacgaagat gcggcagcag gaacgcgcag cgtgaccgag cggttgcggc aggcgggcat
     4621 cgcggtacag gacgtgcacg aagagccgtt ttccctagag gacgttttca tcgccgtggt
     4681 cgagaaagcc cgtgctcacg gcaagtttgc ggaggaggat taaatgttcc ggcgaatcat
     4741 tgcccaagcc cgcaaggaat tgacccagat catgcgcgac cggctggcgc tcgctctggc
     4801 cctggtattg ccgctgtttc tcctgctttt gctcagcaac gcgatttcgc tgacggtcaa
     4861 cgacctgccg ctgatcgttc aggacctgga caactccgcc gcttcgcgca gctacatcga
     4921 tgccttccgc gcctcggtga ccttccgagt ggtttcctgg ccggccaacc gccaacctga
     4981 aagcgcgctg caagccggag cggcacgcgc cgtcctcatc atcccggaac acttctcgcg
     5041 cgatatggcg cgcggccaga cggccaatgt gcagatactg atcgatgcca ccgatgcaaa
     5101 caccgccaaa ctggtcagcg gctacgccgg acagattacc cgggcttacg tcgagcagaa
     5161 taccggcgcc ccctcggttg cgatggtcaa tccgcaggtc cgcctctggt tcaatcccgg
     5221 acgggtctcg caaaagttct acgcgccggg gatcttcgtg ctgggcattt ccatgttccc
     5281 accgctgttg gcgtcgctcg ccatggccaa agagggcgat cagaaaacaa ttctccaggt
     5341 ctatgtttcc agcatctcgg cgcatgagtt tgtgctcggc aagatcctgg ccttcaccgt
     5401 catcgccctg gctgagggcc tgctaaccat gacgaccttg ctgacggttt tcggtttgcg
     5461 actgaccggc gatcccacgc cgctcatcgt ggccaccatt ctgtatgcct tctgcgtggc
     5521 ggcatttggc acgatggttg gggcggccat ccccaaccag gcagcagcca ttcaagcggt
     5581 ggcgctgggc ggcttcttgc tcgtgttcct gctctcgggg ctcatcttcc ccgtggaaaa
     5641 cattcccgtc ggcctgcgct ggatctcgaa cctgatctgg ggccgctact acatcgtgat
     5701 cgtgcgggat gccttactgc aaggcggcgg ctggccggca atgtggttca acgtactcat
     5761 gatcgccatc atcggcgcgg tcttctacgt tctggcgtgg cgggtcttgc gcccgatgca
     5821 ggtgaaggca tgaactccaa cctctccact ccgagcttct ggcagcgcct gcgggcgctg
     5881 atgcagaaag agttcaacca gattcgccgc gaccggcggc tggccatctc gctgatcgtt
     5941 ccacccacgc tgcaaatcct gctgttcggc tttgccctgg actccaacgt ctcgaacctg
     6001 cggctgggag tactggacga gagcaagacg cccgaaagcc gtgagctggt cgccacgctg
     6061 accgaaagta agagcttccg tctgggcggt tacttcttcg aagaccgcac tctggagaag
     6121 gccatcagcc agggcaaggt ggatgccggc gtcgtgattc cctacgattt cgcgcgcgac
     6181 ctgcatcgcg ggcggccgtc cgatatccaa ttcatcctca atgcggtgaa cgcgaatacc
     6241 gcagccatcg ggcagggcta tgccgaaggt gtcctggcca actacaaccg gtcgcttgcg
     6301 tcacagggga tccacccgca aatccgtatc gtggctgctg ataacagccg taaggggtcg
     6361 gtgaatctcg ttccggcatt tctgttcaat ccaggactgg taacggcgtg gttcattgtt
     6421 accggcacgt ttggcacgct actagtgctg aatggctcgc tggtggcttc cgcggccatg
     6481 gtcaaagagc gcgaagccgg cacggttgag cagttgctga tgacgcccgc cgggactacc
     6541 gagatcatcg ttgcgaaaat caccccgatg tttgtcttgc tcctcggcat ggtgtttctc
     6601 gcgctcacgt tgattcgttt cgtgtttggc gtgcctatcc gcgggagctt ggtgctggtc
     6661 tttggcggcg cgatgctgtg cctactatgt ggcatcggta tcggaacctt catcgcgacg
     6721 ttcacccgct ccgcgcaaca ggcacaactc ctggcctttt ttgtaaatcc gcccttggcg
     6781 actctttccg gatcgctgtc gcccgtagaa gccatgccga aatggctgca accgatcacc
     6841 atgttgaacc ccattcgtca cttcgggatc attactcgcg gagccatggt caagggcagc
     6901 accttcgcgg agctatggcc taattttctc gccctgattg ccttcgccat cgtgctgatg
     6961 ggattcagca tttggagatt ccgcaagcag cttggttaag caaacgtcgg agattacttc
     7021 tcatgaacca gcagtggatt aaacagattt ggaccgctat gtgcttctgc ctactttgtt
     7081 gcggagtgtg cttcgcgcag cagcagggag gctccagtac ccagatcacc ctggcgaacc
     7141 aagtggcact ctcggcgcgc accgcgcaga gcggctcggt cacctcgcag caggcgcctg
     7201 tgcccggcac cacgcagagc gtgaacacgc tcaattccac cgttctgacg acgggatcgt
     7261 actcccaaag cgtgctgtcg ggcaaaccgc tgacaggccc actctcgctc aaggaagccg
     7321 taggccgcgg tctcgactac aacctcgggg ccgtcggcct ctccaatgct tccatggcag
     7381 cccatggcca aatgcgagtc gctcgtagca tcctgctgcc caatttgaac ggcaacttgc
     7441 gcgaagccgt gcaacaaacc aacctcgcga ccctgggact gcgctccaat ttcattccgc
     7501 agatcgttgg cccctacaac tacttcgatc tgcgcgccac cctgacccag aacgtagccg
     7561 accttaccgc gttgaacaac taccgttcgg cgcaggaaaa cctgaaagcc gcgcagatgg
     7621 ccgttaagga cgcgcacgat ctggtggtcc tggcggtcgg cggagcgtac ttgcaggtga
     7681 tcgcggccc
//
LOCUS       DASRKK010000396         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_127722, whole genome shotgun sequence.
ACCESSION   DASRKK010000396 DASRKK010000000
VERSION     DASRKK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127722"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..460
                     /locus_tag="VFC15_08990"
     CDS             248..460
                     /locus_tag="VFC15_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10330.1"
                     /translation="MRTLKMLTSGTKLGPYEILAPIGAGGMGEVYRARDIRLDRTVAI
                     KVLPESFASDAERLQRFEHEARPQRA"
     gene            435..1457
                     /locus_tag="VFC15_08995"
     CDS             435..1457
                     /locus_tag="VFC15_08995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM10331.1"
                     /translation="MRRVLSALNHPSLLSIYDVGEQQGLHYLVSEFLEGQILGERIGG
                     RPLSQRRSINYALQIASGLSAAHDKGIIHRDLKPDNIFITRDERIKILDFGLAKQIQA
                     AATAGEGATVTGPTPTIAGMVMRAAGYMSPEQVRAQTLDHRSDIFSFGAILYEMVSGR
                     RAFKGESGIETMNAIVKEEPPELTDSGLPVNPGMERIIRRCLEKSPERRFQSASDLAF
                     AIEALSGVSGSTAAKTVAATSKLGKRITWLTAALVAIVAAVAFAVGMKFAAEPAPTFR
                     QIVSGPGFVSTARFTPDGANAIYGASWNGKPIQIFSTRLDSFESRNLGLPPATCSSDC
                     SHTFMD"
     gene            1486..1719
                     /locus_tag="VFC15_09000"
     CDS             1486..1719
                     /locus_tag="VFC15_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10332.1"
                     /translation="MLTARSASEIAIRRPGQASDRFVDFASNMGDIAGLSADGKLMPV
                     EYAGVGSGADYLSYVANTDTADQKRHRIPSERA"
     gene            complement(1806..1991)
                     /locus_tag="VFC15_09005"
     CDS             complement(1806..1991)
                     /locus_tag="VFC15_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10333.1"
                     /translation="MTITTLSIMAFGMWLPYFPIAWALGFTHLPRLYWPILMLTLLCY
                     LGLTQIIKVWWLGKQWI"
     gene            2215..>3045
                     /locus_tag="VFC15_09010"
     CDS             2215..>3045
                     /locus_tag="VFC15_09010"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZM10334.1"
                     /translation="MNVFVLNSGSSSLKFQVIATDLDRIQQHQDQRVCRGEVERIGGE
                     AIVTFQKGADAKHKFTAPLRDISAALDYLVRYIASDKSGVAEIKSSGDIHAVGHRVVH
                     GGEMFSESALIDGKVLQDIEDCIDLAPLHNPNNIKGIVAARELFGKDVPQVAVFDTSF
                     HHSIPEQAFLYALPYHLYRRHRIRRYGFHGTSHRYVAFRYRALRELTRDQTNIITLHL
                     GNGCSATAIRRGNSVDTSMGMTPLEGLVMGTRSGDLDPAIVNLVATKEGMSPSEVDTL
                     L"
BASE COUNT          648 a          891 c          857 g          649 t
ORIGIN      
        1 cccagcccac ctatcccctg tttacgcttc gcagtacacc tcacggtgca ttacgcaaaa
       61 ctcggggccg agtggttcgc tactccttcc tcgtaggact ctttcattcc ctacttcatg
      121 ccggtttagc ccggcgcacc gcgaaggcga ttttccaaca attgagattg gcgacacggc
      181 caactttcgg tttgccgact atgcatcaga atcgtccgtc tcttcggatg cgctgcacag
      241 gctataattg cgcacactca aaatgctcac ctctggtact aagcttggac cgtatgagat
      301 tcttgcgccc attggtgccg gcgggatggg cgaggtttat cgcgcccggg atattcgact
      361 tgatcgcacc gtcgcaatca aagttctgcc ggagtccttc gccagcgatg ctgagcgcct
      421 gcagcgcttt gagcatgagg cgcgtcctca gcgcgcttaa ccaccccagc cttctatcca
      481 tttacgacgt aggtgagcag cagggacttc attacttggt ctcggagttc ctcgaagggc
      541 agatcctggg cgagcggatt ggaggccgcc cgctctccca gcgccggtcg atcaactatg
      601 ctttgcagat cgcgagtggc ctgtcggcag cccacgacaa gggcatcatt caccgtgacc
      661 tgaagccgga caacatcttc ataactcgcg acgagcggat caagattctg gacttcggcc
      721 tggccaagca gatccaagcc gctgctaccg ccggcgaagg cgctacggtg actggcccta
      781 caccaacgat tgccggcatg gtgatgaggg cggcaggata catgtcgccc gaacaggtgc
      841 gcgcgcaaac gctggaccac cgctctgaca tctttagttt cggcgccatc ctttacgaaa
      901 tggtatccgg ccggcgcgcc ttcaagggtg agtcgggcat agagacgatg aacgccatcg
      961 tgaaggagga gccgccggag ttgaccgaca gtggccttcc tgttaacccg ggcatggagc
     1021 gcattattcg tcgctgcctg gagaaatcac ctgagcgacg gttccagtct gcaagcgatc
     1081 tggcttttgc catcgaagcg ctttctggtg tgtccggcag taccgccgcc aagacggttg
     1141 cggcgacctc aaagctgggc aaacgaatta catggcttac cgcggcactg gtagcgatag
     1201 tggcggcggt cgcgtttgcg gttggtatga agttcgccgc tgaaccagcc ccaaccttcc
     1261 ggcagattgt ttccggtccc ggattcgtca gtaccgcgcg gttcactccg gatggagcca
     1321 acgctatcta tggcgcgtcg tggaatggca agccgatcca aatcttctcc acacgcctcg
     1381 acagtttcga gtcgcgcaat ctcgggcttc caccggcaac gtgttcttct gactgctccc
     1441 acacatttat ggattagcga cattaacagc cgcggacaag tgctgatgct cacggcacgc
     1501 tctgccagcg agattgctat tcggcggccc ggacaggctt cggaccggtt cgtcgatttc
     1561 gccagcaata tgggcgacat tgcaggactg tcagctgacg gtaagctaat gcccgtggaa
     1621 tacgcaggtg tgggctcggg cgctgactac ctctcttacg tcgccaacac ggatacggct
     1681 gaccagaaac gccatcggat tcccagcgag cgggcttaac cgcaacccaa accctgttca
     1741 ggctgccttg gatataccgg ccttcgggta ctggcgaata ccgccgtgtc gccggaaatg
     1801 ccggttcaga tccattgctt tcccagccac cacactttga tgatctgggt aaggcccagg
     1861 tagcagagca gcgtgagcat caggattggc cagtacagcc gcggcaagtg cgtgaagccc
     1921 agtgcccagg ctatggggaa atacggcaac cacatcccaa aagccatgat ggataaggtt
     1981 gtgatggtca acgaccggct ggctcagctc tgaaggaagg gaattttttg tacgaatgac
     2041 atggatgatg agcgtctgcg tcatcaacga ctcgacgaac cagccggtct gaaatagcga
     2101 cgctcgggat ggattccaac acgccgaaga gctcatcacg gttgccaccc agtccaaaaa
     2161 agcgtaggcg cagagacttg ctggtacgat atttcccaag agagaaagcc cggcatgaac
     2221 gtttttgtac tcaattccgg gagttccagc ctgaagtttc aggtcatcgc caccgatctg
     2281 gatcgtatcc agcagcatca ggatcaacgc gtctgccgcg gcgaggtgga acgcatcggt
     2341 ggggaggcga ttgtcacgtt ccagaagggc gccgacgcaa aacacaaatt cacagcacct
     2401 ctgcgagaca tttcggcggc gctggactac ttggttcgct acattgcctc cgacaaatcg
     2461 ggagtcgctg aaatcaagag ttcgggtgac attcacgcgg ttgggcatcg cgttgtgcac
     2521 ggcggcgaga tgttctcgga gtcggccctt atcgacggca aggtattaca ggatattgaa
     2581 gactgcatag atcttgcgcc gcttcacaat ccgaacaaca tcaagggcat cgtggctgcg
     2641 cgagaactct tcggcaagga tgttccccaa gtggccgtat ttgatacttc gtttcatcat
     2701 tcgatccccg agcaagcttt cctgtacgcc ctcccatatc acctgtatcg gcgtcaccgc
     2761 atccgccgat acggcttcca tggaacctcg catcgttacg ttgcgtttcg ctaccgtgcg
     2821 ttgcgcgaac tgacgcggga tcagaccaac atcatcaccc ttcatctcgg gaacggctgc
     2881 tcagcaaccg caattcgccg aggaaattcg gtggatacct ccatgggcat gaccccgctc
     2941 gagggtctgg tgatgggcac gcgttcggga gatctcgacc ctgcgatcgt gaatctcgta
     3001 gcgacaaaag aagggatgtc gccgtcagag gtcgatacgc ttctg
//
LOCUS       DASRKK010000397         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_519371, whole genome shotgun sequence.
ACCESSION   DASRKK010000397 DASRKK010000000
VERSION     DASRKK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519371"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1491
                     /locus_tag="VFC15_09015"
     CDS             136..1491
                     /locus_tag="VFC15_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM10335.1"
                     /translation="MTECNQEVFSFASHFSRRVEASFTAGQVSTDGGALLLRQADGKI
                     GMLKRVASCFTDRRSPLLVEHQLGEMLSQRIYGLALGYEDLNDHEQLRHDPLMAVLSG
                     RRKLEDPLAGKSTLNRLELPGGSQRYHKIDYVPEKIDALLCDLFIESQAEAPDQIVLD
                     LDATDIPLHGHQPERFFHGYYDSYCYLPLYIFAGDQLLCARLRPSNVDGAAGALDEVK
                     RIVAQLRSRWPKVRIVLRGDSGFCREELMAWCEAAENQVDFLFGLARNSRLQKIIGKQ
                     MHEATLLHAETGKAARVFTEFDYQTHKSWARTRRVVAKAEYLDKGENPRFVVTSLSTD
                     EWAARELYEKFYCLRGEMENRIKEQMCLFAYRLSTEEMKGNQLRLYLSALAYTLVEAL
                     RRLALKGTEWAEAQVDTIRLRLFKIGALVRISARRVWLELSSTYPWKQIYARAFDALR
                     C"
BASE COUNT          434 a          597 c          617 g          442 t
ORIGIN      
        1 tagcctgcat atctcacatg ccgactgtta aaggggagag tctgcgggca aaaatatagt
       61 tggaaaggcc gtcttttctg tgtcataact acgttgtcga agcgtgttat ggctgcggaa
      121 agggcggcct ttctcatgac agagtgtaat caagaagtct tctcgtttgc atctcatttt
      181 tcgcgccgcg tagaggccag tttcacagcg ggtcaggtat cgaccgatgg cggagcgctg
      241 ttgttgcgtc aggccgatgg caagatcggt atgctgaagc gggtggcgtc ttgttttacc
      301 gaccgtcggt cgccgctgct ggtcgaacac caacttggtg aaatgctctc gcagcgcatc
      361 tacgggctgg cgctgggcta cgaggacctg aacgatcatg agcagttgcg ccacgatccg
      421 ctgatggccg tgctgtcggg ccgtcgcaag ctggaagatc ccttggccgg caagagcacg
      481 ttgaaccggc tggaacttcc cggcggctcc cagcgttacc acaagatcga ttacgtgccg
      541 gagaagatcg acgcgctgct ttgcgatctg ttcatcgaat cgcaggccga agcgcccgac
      601 cagatcgtgc tcgacctgga tgcaaccgac atcccgctcc acggccatca gccggagcga
      661 ttttttcatg gttactacga cagctactgc tacttgccgc tctatatctt cgctggcgat
      721 caactgttgt gtgctcggct tcgccccagc aatgtagatg gcgcggccgg tgcgctggat
      781 gaagtcaagc gcatcgtggc ccagttgcgc tccaggtggc ccaaggtgcg catagtgctg
      841 cgcggcgact cgggcttctg tcgcgaggaa ctgatggctt ggtgcgaggc cgcagaaaac
      901 caagtggact tcctgttcgg tctggcgcgc aacagccggc ttcagaagat catcggcaag
      961 cagatgcacg aagccacgct gctgcacgcc gagaccggca aggcagcgcg tgtctttacc
     1021 gaattcgact atcaaacgca caagagctgg gcacggacga gacgagtagt tgcaaaggct
     1081 gagtatctgg acaagggcga aaacccacgc ttcgtcgtta cctcactcag caccgatgag
     1141 tgggcggcgc gcgaactgta cgagaagttc tactgcctgc gcggcgagat ggagaaccgc
     1201 atcaaggagc agatgtgcct gttcgcctat cgcctctcga cggaagaaat gaagggcaat
     1261 cagctgcggc tgtacctgtc ggcgctggct tatacgctgg ttgaagcgct gcggcggctg
     1321 gcgctgaagg gcaccgagtg ggccgaagcg caggtggata cgatccggct caggctgttc
     1381 aaaatcggcg ccctggtacg catcagcgcc cggcgcgtgt ggctggaact cagttccacc
     1441 tatccgtgga agcagattta cgctcgggcc ttcgacgcgc tgcgatgctg acctatcctg
     1501 ccgccctcaa tcgttttttc acgccggcca gcaaacaagc ccggcggagc tatgcggaaa
     1561 atcctgcttc gagcctgaaa aagacctcca agcaagcgcc aaacacagac tgaagactgc
     1621 tccgagaccg aatgaccact cttctgccct accagaccac ccagctgaag gatgaaatcg
     1681 gtccgtgtga gaaatcgcgg ctagcggtcc tcgctatgca catagccagc caccttttgt
     1741 atgggtccgt cactgcaaat tcgctcgcaa ttattgcggc aagtctaatc ctcgcgtttg
     1801 ctggttttgt tgccacgttc atccctgcat accgtgcggc cttcgccgat ccgttggaat
     1861 cgctccgtag tgaatgagcg ccgattgatt gctgattaac aggccaatgc cctcattgag
     1921 gaaacgtgac gatctttttg aactccgacg ggcactttgc taaggacgct cagtaaatgc
     1981 aggaagggct ggggtcgcct ggcggctccc aacccatctc agacggtcca tgcgcttgtg
     2041 gcgaacgtcg ccgtcagcga ctcattacgg atgcttcgcc cacgtaagac
//
LOCUS       DASRKK010000398         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_127792, whole genome shotgun sequence.
ACCESSION   DASRKK010000398 DASRKK010000000
VERSION     DASRKK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127792"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFC15_09020"
     CDS             <1..192
                     /locus_tag="VFC15_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10336.1"
                     /translation="PSPVMGGGENSGLDPAMLHAFFWNLLAFTCVGIVLMAVRYRLQM
                     LEEEVEAAHAAAALQGGAQ"
     gene            189..320
                     /locus_tag="VFC15_09025"
     CDS             189..320
                     /locus_tag="VFC15_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZM10337.1"
                     /translation="MTGPVALKAAYIVTWVILLGYLGSLLRRFRRVREEMKDLKRGG"
     gene            470..982
                     /locus_tag="VFC15_09030"
     CDS             470..982
                     /locus_tag="VFC15_09030"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZM10338.1"
                     /translation="MNSEKHGVLVFLGVANFILCTLFVILSVPFFWLQYQVLQTWPET
                     EAQVIRSEVVMQPAPPHEQLYAAELQVLYTVDGRPITAELISFQSQNYQQTADRAAQF
                     PVGSRHPIRYDPHVPTQARIGAGWNLRFFLVPLVMLGMGAIFGAIAAVFLVAAKFAVA
                     PVILPPPSLS"
     gene            complement(1056..1469)
                     /locus_tag="VFC15_09035"
     CDS             complement(1056..1469)
                     /locus_tag="VFC15_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM10339.1"
                     /translation="MSARSFFDTNVLVYSDDKAAPAKQRRALDLLAEHRRGGTGVVSL
                     QVLQEYFVTITWKLHVDARIARRKVELLAEFDVAVPNVADILAAIDLHRLHGFSFWDA
                     LVLRAAKQAGCSVLLSEDLQEAREIDGIRIVNPFR"
     gene            complement(1456..1677)
                     /locus_tag="VFC15_09040"
     CDS             complement(1456..1677)
                     /locus_tag="VFC15_09040"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HZM10340.1"
                     /translation="MNVTLSIDEQLVTRARKKADALGKSLNQLIRDYLQKLAGGDDPE
                     QSIQEFKHLSGRGHSRGWRFDRNEIHERP"
     gene            2083..2685
                     /locus_tag="VFC15_09045"
     CDS             2083..2685
                     /locus_tag="VFC15_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZM10341.1"
                     /translation="MLTDLTDEQLLAQALAGDEDAFTALYRRRQGPVFRFALHMSGSS
                     SIAEEVTQDVFMFLIQRGRDFDPSRAALGAYLFGVARNYVRRALERNYAETALSSPVD
                     DEAQSLTVNSDPGEGIARQQASTAVWKAVLSLPEHYREVVVLCDLQELTYADAAEAVG
                     CALGTIRSRLHRAHDMLSRKLQHSAAAAPGISSVTARCLS"
     gene            complement(2736..>2976)
                     /locus_tag="VFC15_09050"
     CDS             complement(2736..>2976)
                     /locus_tag="VFC15_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10342.1"
                     /translation="AEPHGDWSETVRHLRPHSLGGSLPECPEQRPFHIGASRAAGGSR
                     LQRLHRGREHSLLLQPGCAFLAPARMAQDLLILGS"
BASE COUNT          607 a          853 c          883 g          633 t
ORIGIN      
        1 ccttcgccgg tcatgggagg cggcgaaaac tcgggtctcg atccagcgat gctgcacgcc
       61 ttcttctgga acctgctggc gttcacctgc gtgggaattg tcctgatggc ggttcgttac
      121 cgcttgcaga tgctggaaga agaagtagaa gccgcgcacg cagcggcagc cttgcaagga
      181 ggagcacaat gaccggccct gtcgcattga aagccgccta catcgttacc tgggtgatac
      241 tgctgggcta cctcgggtcg ctgcttcgac gtttccgccg ggtccgcgaa gagatgaagg
      301 acctgaagcg tgggggataa gacgaatcac cacagagaca cagaggcaca gagaagaaaa
      361 gaattccttt tttaccacgg aggacacgga gatcacggag tttcaattgt tatttcttct
      421 ccgcgccctc cgtgtcctct gtggtgaacc cgatttctag gatgcgcgct tgaacagcga
      481 aaaacacggc gttctggtgt tcctcggggt tgccaatttc atcctgtgca ctctgttcgt
      541 gattttgagc gtgccctttt tctggctgca gtaccaggtg ttgcaaacct ggcctgagac
      601 cgaagcgcaa gtcatccgca gcgaggtggt gatgcaaccg gcgcccccgc acgagcagtt
      661 atacgctgcc gagttgcagg tcctctatac cgttgacggc agaccgatca cggcagaact
      721 gatcagcttc caaagccaga actaccagca aaccgccgac cgggcggcac agttccctgt
      781 cggcagccgc catcccatcc gctacgatcc gcatgtcccc acacaagcgc gaattggagc
      841 cggctggaac ctgcgctttt tccttgtgcc actggtgatg ctggggatgg gcgcaatctt
      901 tggcgcaatc gcagccgtct tcctggtggc ggcaaagttc gctgtcgctc cagtgatcct
      961 gccgcctccg tcgttgtcat agagtggtga cgaaacgaag ggactcaccc tgaagggttc
     1021 gttccttcgg tagtgatgcc cgcctgaagc aaatcctatc ggaacgggtt tacgatgcgg
     1081 atcccgtcga tctctcgcgc ttcttgcaag tcctccgaca aaagtacact acatccagcc
     1141 tgtttcgcgg cccgtaggac cagagcatcc cagaaggaaa acccgtgaag gcgatgaagg
     1201 tcgatggcgg caaggatgtc ggcaacgttg gggacagcaa catcgaactc ggcgagcaat
     1261 tcgactttcc tgcgagcaat gcgcgcatca acgtgcagtt tccacgtgat ggtgacgaaa
     1321 tactcttgca gcacctggag cgacacaacg ccggttcccc cacgccggtg ctctgccagg
     1381 agatcgaggg cgcgtcgctg cttcgctggt gcagccttgt cgtccgagta aacgagaaca
     1441 ttggtgtcga agaagctacg ggcgctcatg aatctcgttt cgatcgaagc gccagcctcg
     1501 agaatgaccc ctacctgaga gatgtttgaa ctcctggatg ctctgctcgg gatcatcgcc
     1561 gccggccagc ttttgcaggt aatcacggat aagttgattg aggctcttgc cgagggcgtc
     1621 ggccttcttt cgagcgcgtg tcacaagctg ctcatcaata gacaatgtca cgttcattaa
     1681 cacagtatat gtgcgcatgt atcatgtgtc aacttgacgc aaccgggaaa tgaatcggcc
     1741 attacttacg cagcacctga acagtccgag gagcgagagc tcacccaatc atctccgctc
     1801 gcgtcacatc gagagggtga tcgatcagtg gcgccacgcg ttcgatgaat cgcaccgtat
     1861 gcggcaatct ggcgtgattc ttagcagcaa tcaacccgaa gcgtcacttt tttcagccgc
     1921 ctgtccaagc gctgtcccaa cagcgccttt tcccatttca cggtgttcct ttattggtag
     1981 gcccgaggga atacgataaa gaagtctaac tttacggaac tttccgtttc ggctcgggga
     2041 aatactgacg gaaacgagat cgggttcacc actgaggtgg agttgctgac cgacctaacc
     2101 gacgaacagt tgcttgcgca agcgctggcc ggagatgaag atgccttcac tgccctctac
     2161 cgcaggcggc agggtcccgt ctttcgcttc gccttgcaca tgagtggctc gtcgtcgatc
     2221 gcggaagagg tgacccagga tgtttttatg ttcctgattc agcggggccg cgacttcgac
     2281 ccgtcccggg ccgcgctggg agcttacctg ttcggcgtag cccgcaacta tgtgcggcgg
     2341 gcgctggagc gcaattatgc ggagacggcg ttgagcagtc cggtggatga cgaagcgcag
     2401 tcgctgaccg tcaacagcga tcccggagaa ggaattgccc ggcaacaggc ttcgactgcg
     2461 gtgtggaagg ctgtcctttc tctgccggaa cattatcgcg aggtggtggt gctctgcgat
     2521 ctccaggagt tgacttatgc agatgcagcg gaagcggttg gctgtgcgct gggcaccatc
     2581 cgttcgcggc tgcaccgggc gcacgacatg ctcagtcgga agctgcaaca ttcggcggcg
     2641 gctgcacccg gaatatcgag cgttacggcg aggtgtttgt catgacgtgc cgtgaatttg
     2701 aacatcgtgc tacctctctg actttgtggg agctgtcacg agcccaggat cagcagatcc
     2761 tgggccatgc gggcgggtgc cagaaatgcg cagcctggtt gcaacagcag cgaatgctcg
     2821 cggccacgat gcaaacgttg caagcgcgaa ccgccggctg cgcggctggc cccgatgtgg
     2881 aacgggcgtt gctccgggca ttccggcagg gaccctccca ggctgtgcgg ccggagatgg
     2941 cgcaccgttt cactccaatc gccatgcggc tcagcc
//
LOCUS       DASRKK010000399         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_30687, whole genome shotgun sequence.
ACCESSION   DASRKK010000399 DASRKK010000000
VERSION     DASRKK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30687"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1159
                     /gene="prmC"
                     /locus_tag="VFC15_09055"
     CDS             191..1159
                     /gene="prmC"
                     /locus_tag="VFC15_09055"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZM10343.1"
                     /translation="MQLKQALASAVERLDAADVGSPRMNAEVLLMFVLGADRAYLYAH
                     PERELTPEEAARYEEVLAQRATGMPSQYITGHQEFWGLDFVVSPAVLIPRPETEHLVE
                     AVLELARGIERPKLVDVGTGSGCIALALAHELQDAEVFAVDLSAEALEIARANAARLQ
                     LDKRVRFLQCNVLDPMLDQNPPVIPSDAFSPSLEPAQSLSKGSCFSEFCDFDFVVSNP
                     PYVAFSEADKVQRSVQEFEPTMAVFAGLHGTDVIVPLIEQAHRALKPGGWLALEIGYS
                     MRNDVVNLLSPTMWDDVRVMPDLQGIPRVVAAKKKAISPSHGSAVL"
     gene            complement(1125..1535)
                     /locus_tag="VFC15_09060"
     CDS             complement(1125..1535)
                     /locus_tag="VFC15_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM10344.1"
                     /translation="MNYLLDSSICIALLRDRSIPVKQRFREAITGRAMIWISSVVLHE
                     LWYGVHKSSRPVENAEALRRFLHGPVSVLRFDGEDAQVAGEIRAELERTGKTIGSYDT
                     LIAAQCLRNDLIVVTSNLSEFRRVRGLRCHDWAR"
     gene            complement(1532..1774)
                     /gene="vapB"
                     /locus_tag="VFC15_09065"
     CDS             complement(1532..1774)
                     /gene="vapB"
                     /locus_tag="VFC15_09065"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZM10345.1"
                     /translation="MTKIAKLFRNGNSQAVRLPKEMRFKGDRVRVRKIGDTVVLEPVI
                     ANVEEWFAEMDRHPLSEDFMAGGRKQPKAPKRKLFE"
     gene            complement(1861..>3346)
                     /gene="purH"
                     /locus_tag="VFC15_09070"
     CDS             complement(1861..>3346)
                     /gene="purH"
                     /locus_tag="VFC15_09070"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZM10346.1"
                     /translation="VELISTGGTAKLLRDSGIAVKDISELTGFPEMLDGRVKTLHPKV
                     HGGILHIRKNEKHVAAVNQHGIQPIDMVVVNLYAFEKTAAKPGVEFEDVIENIDIGGP
                     SMVRSAAKNFQDVAIVTSPVDYEAIAQEMESSEGSLSLKTKWRLAQKAFATTAAYDSA
                     IASTLERIGAPETDGEKFELGKDSGFPETLRLSLHKAFDLRYGENPHQKAALYSDGSG
                     KGIANGKPLQGKELSYNNIVDLQAAWDLAQEFEEPVCAIIKHTNPCGTAVGATLAEAY
                     KRALECDPVSAFGGVIGVNRPVDRETASEMAKLFVEAIAAEGFDPAAREIFAAKKNLR
                     LVEVVPMEQKWVLKNVSGGVLLQDNDMRPLTEADLKVVSERKPTEPEMRDLLFAWKVC
                     KHVKSNAILYARNGQTTGVGAGQMSRVDSARIGAMKSVLGIKGSVAASDAFFPFPDGV
                     EEIAKAGATAIIQPGGSVRDQEVIDAANRLGLAMVLTGVRHFRH"
BASE COUNT          647 a         1021 c          956 g          722 t
ORIGIN      
        1 cacggagccg caggcggagt gctgggtaaa gtggaaaata gatccgagtc ccgtagggac
       61 ggtagaggtt gcttctctcg attcttggaa tcccgtcgag gcagcacgga agaatgcgga
      121 tgtgccgtgc ctacgacgct cgggttcttt gaggcgactt gaacgcggcc caaggtgcgt
      181 tgcgaatccc gtgcaactaa aacaagcgct agcgtccgcg gtggaacgtc tcgacgctgc
      241 cgacgtgggc tccccccgca tgaacgccga ggtgctactt atgtttgtac tcggcgccga
      301 tcgtgcctac ctgtacgcgc atcccgagcg cgaactcact ccagaagaag ctgcacgcta
      361 cgaagaagtg ctcgcccagc gcgcaaccgg catgccatcg cagtacatca ccgggcacca
      421 ggagttctgg ggcttggatt tcgtggtctc gccggcggtg ctgattccgc gtcccgagac
      481 cgagcatctg gttgaggctg tactggagct ggcgcgcgga atcgagcgtc cgaagctggt
      541 ggatgtgggt acaggttcgg gatgcatcgc actggcactc gcgcacgaat tgcaggacgc
      601 cgaagtcttt gccgtcgatc tctcggccga agcgctggaa atagcgcgcg ccaatgccgc
      661 ccggctgcaa ctcgataagc gtgtgcggtt cctgcaatgc aatgttctcg atcccatgct
      721 tgaccagaac cctcccgtca tcccgagcga tgccttcagt ccgagcctag agcctgccca
      781 gagcttgtcg aaggggagct gcttttccga attttgcgat tttgattttg tagtcagcaa
      841 tccgccgtac gtcgctttca gcgaagctga caaagtgcag aggtcggtgc aggagtttga
      901 accgacgatg gcggtcttcg ccggcttgca tgggaccgat gtgattgtcc cgctgattga
      961 gcaggcgcac cgcgcgctga aacctggcgg gtggttagcg ctggagatcg gctactcaat
     1021 gcgaaacgac gtcgtcaacc tgctgagtcc cacaatgtgg gatgacgtcc gtgtcatgcc
     1081 tgacctgcaa gggattccac gggtggtggc ggcgaagaag aaggctatct cgcccagtca
     1141 tggcagcgca gtcctctgac tcggcgaaat tcagagaggt tcgatgtcac gacaatcaaa
     1201 tcgttgcgca agcactgggc tgcgatcaga gtatcgtacg agccaatcgt tttgccagtt
     1261 cgctccagtt ctgcccgaat ctcgccggcc acttgcgcat cttctccatc aaatcgcaag
     1321 acgctcaccg gaccatgcag aaatctccgc aaggcctcag cattttcaac cgggcgcgag
     1381 ctcttgtgta ctccgtacca tagttcgtga aggactactg acgaaatcca aatcatagcg
     1441 cggccggtga tggcctctcg aaaacgctgc ttgaccggaa ttgatctgtc acgcagcagc
     1501 gcgatgcaaa tgctgctatc gagcagatag ttcattcaaa caacttccgt ttcggtgcct
     1561 taggctgttt acgaccgccc gccatgaagt cctcggacag aggatgccgg tccatttccg
     1621 caaaccattc ctcaacgttg gcgatgacgg gttccaaaac cacggtatcg ccaatctttc
     1681 gaacgcgcac acgatcgcct ttgaaccgca tctccttggg cagacgtacg gcctggctgt
     1741 ttccgttacg aaacaacttg gcgattttgg tcatggacct gttctccaat cgcggtatat
     1801 attatcagtc tatactacgc caagggttcc aaccctatac ccagtcggcg agcgaagcct
     1861 tcaatgccgg aagtgtctta ccccggtgag caccatcgcc aggcccaacc tgttcgccgc
     1921 gtcaatcact tcctgatcgc gcacagagcc gccgggctga atgatggccg tcgcgcccgc
     1981 cttggcaatc tcctcgacgc cgtcagggaa cgggaagaac gcgtccgatg cggcaaccga
     2041 tcccttaatt ccgagcaccg acttcatggc gccgatcctt gccgagtcca cgcggctcat
     2101 ctggcccgcg ccgacgccgg tggtctgacc gttgcgcgca tagagaatcg cgttcgactt
     2161 cacatgcttg catactttcc acgcgaacag caggtcgcgc atctccggtt ccgtgggttt
     2221 gcgttcgctc accactttga ggtccgcttc ggtcaagggg cgcatgtcgt tgtcctgtag
     2281 caggacgccg ccggagacgt tcttgagcac ccacttctgc tccatgggaa ccacctcgac
     2341 caggcggaga ttcttcttcg cggcgaagat ctcgcgcgca gcaggatcga acccttcagc
     2401 agcaatcgcc tccacaaaca gcttcgccat ctcactcgcc gtctcgcgat cgaccggccg
     2461 gttcacgcca atcaccccgc cgaacgcgga caccggatcg cactccagcg cgcgcttata
     2521 agcctccgcc agagtggctc ccactgcggt cccgcaggga ttggtgtgct tgatgatggc
     2581 gcacaccggc tcctcgaatt cctgcgccag gtcccacgct gcctgcaggt caacgatgtt
     2641 gttgtagctg agttccttgc cctgcaacgg cttgccattg gcaatgccct tgcccgagcc
     2701 gtcggagtac agcgctgcct tctgatgcgg gttttcgccg tagcgcagat cgaaggcttt
     2761 gtggaggctg agtcggagag tttcgggaaa gccgctgtcc ttaccgagtt caaacttttc
     2821 gccatcggtc tcgggagcgc caatgcgctc gagggttgac gcgatcgcag agtcgtaggc
     2881 cgcagtcgtg gcaaatgctt tttgggccag gcgccacttc gttttcagcg agagcgatcc
     2941 ctcactgctc tccatttctt gcgcaatcgc ttcgtagtca accggcgagg tgacgatggc
     3001 gacgtcctgg aaattcttgg ctgccgaccg caccatcgac gggcccccga tgtcgatgtt
     3061 ttcgatgacg tcctcgaact cgacccccgg tttcgcggca gtcttctcga acgcgtagag
     3121 attcaccacc accatgtcga tgggttgaat gccgtgctgg ttcactgccg cgacgtgctt
     3181 ttcattctta cggatgtgga ggatgccgcc gtgcaccttg ggatgaagcg tcttgacgcg
     3241 gccatcgagc atctccggga agccggtaag ctccgaaatg tccttcactg cgataccgga
     3301 atcgcgcagc agcttggccg tacctccggt tgaaatcagt tcgacg
//
LOCUS       DASRKK010000400         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_127880, whole genome shotgun sequence.
ACCESSION   DASRKK010000400 DASRKK010000000
VERSION     DASRKK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127880"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFC15_09075"
     CDS             complement(<1..1326)
                     /locus_tag="VFC15_09075"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZM10347.1"
                     /translation="MSSLTSDTETMTEKKTAVKHKQLAAWVKEVTQICKPDQVHWCDG
                     SHEEYQAMLQRMVLTGSAIWLAGDKRPNSILVRSSPGDVARVEDRTFICSRTQEEAGP
                     TNNWEDPAKMKQKLQGLLAGAMTGRTMYVIPYSMGPIGSPIANIGVELTDSPYVVANM
                     HIMARVGTRVLEVLGSDGDFVRALHSVGAPLAANEADSAWPNNAKEKYICHFPETREI
                     WSYGSGYGGNALLGKKCHALRIASVQARDEGWMAEHMLILKMTDPSGRVKYFTGAFPS
                     ACGKTNLAMVVPTIPGWKVETVGDDLAWMKFGADGRLYAINPEYGFFGVAPGTSMQSN
                     PNAMLAATKNSIFTNCAMTPDGDIWWEQMTDEKPSELIDWLRRPWTPNCSRKAAHPNS
                     RFAVPARQCPVIAPEWEDPEGVPIDAILFGGRRAGVVPLVTESCSWQHGT"
     gene            complement(1624..2583)
                     /locus_tag="VFC15_09080"
     CDS             complement(1624..2583)
                     /locus_tag="VFC15_09080"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZM10348.1"
                     /translation="MTNGNSHFDSVCIKSTGSYVPSKILTNEEVLANLPTSPAWVVET
                     LGIRQRHIAAPDEFTSDLAARAGLEAIASAGLEPNDIELIIVSTATPDRKMPSSACSA
                     QVKMGIKNRCAAFDVAAVCAGFVYGITIGGQFIQNGMYEHVLVIGADTFSKVTDWNHR
                     DCVVFGDAAGAVVLERKDREKGLFSSILLAEGEGKDHFTVYPGDAYFKMNGKAVYQAA
                     TTVVPECILEILRINGLGLEDVSVIVPHQATVGVLKRIAEVLNIPFSRIHTNLDSYAN
                     TAGATVPLLLDQVNRRGLIHEGDLLLLVSIGAGWTWGASLCRW"
BASE COUNT          630 a          905 c          880 g          656 t
ORIGIN      
        1 cgttccgtgc tgccaactgc acgactctgt cacgagtgga acaaccccag ctcggcggcc
       61 accgaaaaga attgcgtcga tcggcacacc ctccgggtcc tcccactccg gagcaatcac
      121 agggcactgc cttgccggaa cggcgaagcg gctattggga tgagctgctt tgcgagaaca
      181 attcggagtc cacgggcgac gcaaccagtc gatcaattcg gacggctttt cgtcggtcat
      241 ctgctcccac catatgtccc cgtcgggagt catcgcgcaa ttggtgaaga tggaattctt
      301 cgtcgcggcg agcatggcgt tcgggttcga ctgcatgctc gttccaggag ccacgccgaa
      361 gaatccgtac tccgggttga tggcgtagag gcggccatcg gcgccgaact tcatccaagc
      421 gaggtcgtcg ccgacggttt ccactttcca ccccggtatc gtcgggacca ccatcgcaag
      481 gttcgtcttg ccgcacgccg aagggaacgc gcccgtgaag tatttgacgc ggccggaggg
      541 gtcggtcatc ttcagaatga gcatgtgctc cgccatccag ccttcgtcac gggcttgcac
      601 cgaagcgatg cgcagcgcgt gacacttctt gccgagcagc gcgttgcccc cgtagccgga
      661 gccgtaagac caaatttcgc gcgtctccgg gaagtggcaa atatatttct ctttcgcatt
      721 gttaggccaa gccgaatccg cctcgttcgc cgccagcgga gcgcccaccg agtgcagggc
      781 gcgcacaaaa tcgccgtcgc ttcccaggac ttcgagcacg cgcgtcccga cgcgcgccat
      841 gatgtgcata ttagccacca cgtaaggcga gtccgtaagc tcgacgccga tattcgcaat
      901 cggcgagcca atcgggccca tgctgtacgg gatcacatac atggtcctgc ccgtcatcgc
      961 acccgccagc aacccctgca gcttctgctt catcttcgcg ggatcttccc agttgttggt
     1021 gggaccagct tcctcttggg tccgcgaaca aataaaggtg cggtcttcga cgcgagcgac
     1081 gtcgcccgga cttgaccgga ccaggatgct gttcggccgc ttgtcgccgg caagccagat
     1141 ggcgctgccc gtcaggacca tccgctgtag catggcctga tattcctcgt gcgaaccgtc
     1201 gcaccagtga acttgatcgg gcttgcagat ctgggtgact tctttcaccc aggcggccag
     1261 ctgcttatgc ttgactgcgg tctttttttc ggtcatggtt tccgtatcgg aggttaggct
     1321 ggacataaga atgctctcct cgaacagcaa actgcacgat cccctatcgc gccatgaaac
     1381 tggcattccc gggcagaaaa gcgaggctgc gcgaagagat tctgcccgtg ccacagaaca
     1441 gtgctaactg atgtccatga ttgcctcaat tgaagttacg tgcgaaaacc attgctgttt
     1501 ccacgattga tatgggaagt gcgtgcacga gaccggccga aactcgattt ccgcgtacta
     1561 gcccaaaacc cgccaaagcg atcagttgcg aaagcacctc ctcggaggtc gtcaagaccg
     1621 ggcttaccat cgacataggc ttgcgcccca ggtccagccc gcgccgatgg acacgagcaa
     1681 gagaagatcg ccttcgtgga tgaggccgcg tcggtttacc tgatccagga gtagcggaac
     1741 tgtggctcct gcggtgttcg cgtacgagtc caggtttgtg tggattcggg agaaagggat
     1801 gttgagtacc tcggcgatgc gcttgaggac gccaactgtg gcctggtgcg gaacgatcac
     1861 agagacgtct tctagtccca acccatttat gcggagaatt tccagaatgc actcgggcac
     1921 cacagttgta gccgcctggt aaaccgcctt tccgttcatt ttgaagtatg cgtcgccagg
     1981 gtagactgtg aaatgatcct ttccctcgcc ctcggcgagg aggatgcttg aaaagagccc
     2041 tttctcccga tccttcctct ccagtaccac ggccccggca gcgtctccga aaacgacgca
     2101 gtcgcggtgg ttccagtccg tgaccttgga gaaagtatcg gcgccgatca ccagaacgtg
     2161 ctcgtacata ccattctgga tgaattgccc gccaatcgtg atgccgtaaa caaacccggc
     2221 acacaccgcc gccacatcga acgcggcgca acggttctta atgcccatct tgacctgggc
     2281 actacatgcc gaggagggca tcttgcgatc gggcgtcgcc gtcgacacga tgatcaattc
     2341 gatgtcgttt ggttcgagtc ccgccgaagc gattgcctcc agcccggccc gggcggcaag
     2401 atccgaagtg aattcgtccg gtgcagcgat gtgccgctgg cgaatcccca aggtttcgac
     2461 aacccatgcc ggagaggttg ggaggtttgc cagtacttct tcgttggtca gaatcttgga
     2521 tggaacgtaa gaacctgtgg attttatgca gacactatca aagtggctgt ttccatttgt
     2581 cattttcccc tccagtcccg cgaacagaac gcgatcgcgg aggtgagttc acagcaaggg
     2641 cacttctacc gttacattgc ttgtttgaag cgcgccgatg gggcgcaaac ctatgccgta
     2701 agagttggtg atcctaccgg ctgctatcgt ctcccttggc taagagcgtt tccgtgcatt
     2761 gagtcaccat cctgtgtgat ctttgaccgg aggcagcacc cgggggacgc ctgtgatcgt
     2821 tgtaacgtac gtatgggtag cgtatcccgc ctgtcacttg gcggataatc ggcccaacgg
     2881 aaactgactc ttcaccacgc ctcggatagt tgccgtgaaa tcgccatctg ggtgcgccgg
     2941 gataaaccgg catgaagcag agaatggaaa attcttacga gaaaggagta gcgatccact
     3001 cggccccgag ttttgcgttg ggtatcgtga ggtgcacagc gaagcgtaaa caggggtata
     3061 ggtgggctgg g
//
LOCUS       DASRKK010000401         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_616439, whole genome shotgun sequence.
ACCESSION   DASRKK010000401 DASRKK010000000
VERSION     DASRKK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616439"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VFC15_09085"
     CDS             complement(<1..1930)
                     /locus_tag="VFC15_09085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HZM10349.1"
                     /translation="MNNFGEKVDFIWPVADLLRGPYRPNQYKDVMLPLTVLRRLDCVL
                     EPTKDQVLARANKLEGGKLKNVEPVLCRITGVPFCNTSRYTFEKLKGDPNNIAANLTN
                     YIKGFSIRAREIIERFGFEEHIARLDKADRLYLIVSRFCQVDLHPDRVSNIEMGYIFE
                     ELIRRFNEASNEEAGDHFTPREVIRLMVNLLFMPDSDVLTTRGIVKTLYDPACGTGGM
                     LSVAEDYVRELNPDAQLEVFGQDYNAQANAICGSDMMIKDQNIDHIAFGDSFTDDRFP
                     QHKFDYMLANPPFGVEWKPEADFIKQEREEQGFGGRFGAGLPRINDGSLLFLQHMISK
                     MEDPADGDSGGGTRLGIVFNGSPLFTGAAGSGESEIRRWIIENDWLEAIVALPDQLFY
                     NTGIYTYLWIVTNRKEPNRKDKIQLVDGTSFFMKMRKSLGNKRNEVCDNQRDEITRLY
                     GQLDDASGADHVRIFDNTDFGYQRITVERPLRLSFAVDAERLARLQEAKPFAKLPTSR
                     KRKDTKAAQAEIEAGRKQQDDILAALRTLAGQGVVKDREAFTGQMKTAFKKADLKVPA
                     ALFKAILMALAERDETAEICTDKKGNPEADSDLRDYENVPLKETIKEYMKREVLPHVP
                     DAWVDESKTKIGYEINFNR"
     gene            complement(1968..2255)
                     /locus_tag="VFC15_09090"
     CDS             complement(1968..2255)
                     /locus_tag="VFC15_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZM10350.1"
                     /translation="MIPENRIPTHPGVILSQEFLAPLGISQVAFAAHLRVSVQRINEL
                     IRGKRGITPETAWLLSQALNTTPEFWINLQTAYDLARSRPEEPIERLATVG"
     gene            complement(2267..2560)
                     /locus_tag="VFC15_09095"
     CDS             complement(2267..2560)
                     /locus_tag="VFC15_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM10351.1"
                     /translation="MIVSFGDSATEDLYHKRPTSRALRFPQDIVDTALVKMDLLNGAA
                     AVLDLRSPPGNRLEALKGDLKGLHSIRVNDQWRLVFRWEGSNAYELQLMDYHK"
BASE COUNT          509 a          821 c          953 g          628 t
ORIGIN      
        1 agcggttgaa gttgatctcg tagccgatct tggtcttgga ctcgtctacc caggcatcgg
       61 ggacatgggg gaggacctcg cgcttcatgt actctttgat ggtctctttg agtgggacgt
      121 tctcgtaatc cctcaggtcc gaatcagctt ccgggttgcc cttcttgtcg gtgcagatct
      181 cggccgtctc gtcgcgttcg gccagggcca tgaggatcgc cttgaacagc gcggcgggca
      241 ccttcaggtc ggccttcttg aacgcggtct tcatctgccc ggtgaacgct tcgcggtcct
      301 tgacgacgcc ttgcccggcg agcgtgcgca gcgcggcgag aatgtcatcc tgctgcttgc
      361 gtcccgcttc gatctctgcc tgagcggcct tggtgtcctt gcgcttgcgg ctggtgggga
      421 gcttggcgaa cggcttggcc tcctggaggc gggcgaggcg ctcggcgtcc acggcgaagc
      481 tcagccgtaa cggacgctcg acggtgatgc gctggtagcc gaagtcggtg ttgtcgaaga
      541 tgcggacgtg gtcggccccg gaagcgtcgt cgagttggcc gtatagccgg gtgatctcgt
      601 cgcgctgatt gtcgcagact tcgtttcgct tgttgccgag gctcttgcgc atcttcatga
      661 agaagcttgt gccatccacc agttgaatct tgtccttgcg gttcggctct ttgcggttgg
      721 tgacgatcca caggtaggtg tagatgccgg tgttgtagaa gagctgatcg ggcagggcga
      781 cgatggcctc gagccagtcg ttctcgatga tccagcggcg gatttcgctt tcgcccgacc
      841 cggctgcgcc tgtgaaaagc ggtgagccgt tgaagacgat ccccagacgc gtgccgccac
      901 cggaatcgcc gtcggcgggg tcttccatct tgctgatcat gtgctggagg aacagcagcg
      961 acccgtcgtt gatgcgcggc agacccgcac cgaagcgacc gccgaagccc tgttcctcgc
     1021 gctcttgctt gatgaagtcg gcctcaggct tccattccac accaaagggc ggattggcaa
     1081 gcatgtagtc gaacttgtgc tgcgggaagc ggtcgtcggt gaagctgtcg ccgaaggcga
     1141 tgtggtcgat gttctggtcc ttgatcatca tgtccgagcc gcagatggcg ttggcttggg
     1201 cgttgtagtc ctggccgaag acttcaagct gggcgtcggg gttcagttcg cggacgtagt
     1261 cctcggccac ggagagcatg ccgccggtgc cgcaggcggg gtcgtagagc gtcttgacga
     1321 tgcccctggt ggtcagcacg tcgctgtcgg gcatgaacag caggttgacc atcaggcgga
     1381 tgacctcgcg cggcgtgaag tggtcgccgg cttcctcgtt ggaggcctcg ttgaaccgcc
     1441 ggatgagctc ctcgaagatg tagcccatct cgatgttcga gacgcggtcg gggtgcagat
     1501 cgacctggca gaagcgggag acgatcaggt agaggcggtc ggccttgtcg aggcgggcga
     1561 tgtgctcctc gaagccgaag cgctcgatga tctcgcgggc gcggatggag aagcccttga
     1621 tgtagttggt gaggttggcg gcgatgttat tcgggtcgcc cttgagcttc tcgaaggtgt
     1681 agcggctggt gttgcagaac ggcacgccgg tgatgcggca gaggaccggc tcgacgttct
     1741 tgagcttgcc gccctcgagc ttattggcgc gcgccagtac ctggtctttc gtgggctcca
     1801 gcacgcagtc gagccgacgc aggacggtca gtggaagcat gacatcctta tactgattcg
     1861 ggcggtatgg cccgcggagc aggtccgcga ccggccagat gaagtcgacc ttctcgccga
     1921 aattgttcac aagctatcgc tcctctcgaa tgtccgctcc gcgacgctca gccgactgtg
     1981 gctagccgct caattggctc ctcaggccga ctccgggcca gatcgtatgc ggtctgcaag
     2041 ttgatccaaa attccggtgt ggtgttgaga gcctgcgaaa gtagccaagc cgtctccggc
     2101 gtgataccac gcttgccgcg gatgagttcg ttgattcgct ggaccgatac gcggagatgt
     2161 gctgcgaagg ccacctggga gatgccgagc ggggcgagaa actcctggct taggatcact
     2221 cccggatgcg tggggatgcg attctcaggg atcatggttg acctccctat ttgtgatagt
     2281 ccatcagttg aagctcgtaa gcgttgctgc cctcccaacg gaagacgagt cgccactgat
     2341 cgttgacccg aatcgagtgg agacccttta gatctccttt cagggcctcg agtcgattgc
     2401 ccggtgggga cctcagatcg agcacagcgg cagcaccgtt gagaaggtcc atcttcacca
     2461 acgcggtgtc gacgatgtct tgcgggaacc ggagtgcccg gctggttggc cgtttgtggt
     2521 acagatcctc ggtcgcgctg tccccgaatg agacgatcat caagcctccc tgctcaccgc
     2581 tagactatat cactatcccg gataccgtgt aatttttccg ccgaggcgga ccaggcaccc
     2641 gccactcttc cgttctatca aagctttgag tccctcgcgg accagaccgg ttttctttga
     2701 gtgtgtcaac ccgaaccagg atgggaaccg taaaagctac cgggtaaggc ataccgggta
     2761 aggcaagaag aaggcaagaa gaccacagga agagggtaat catgactgat cgtgcatctg
     2821 aggaaggaaa gaccctgacc accgggcacg gcaggcccgt cgacgatgac caaaactcgc
     2881 aaaccgccgg agggcgcggg ccggttctga t
//
LOCUS       DASRKK010000402         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1399527, whole genome shotgun sequence.
ACCESSION   DASRKK010000402 DASRKK010000000
VERSION     DASRKK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399527"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFC15_09100"
     CDS             <1..591
                     /locus_tag="VFC15_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10352.1"
                     /translation="ADWFPLACVTPFSKLTAALRGTGVPTISSHPHCSLGTYFFVDEK
                     SKKAVPITQFVDVPNMLQDMEELSRSAGKARFKLYSKVKAWSSLQKHFKPQLAPPGLT
                     FTKFLQTLQGMTNKKLGRDGKDGTFTYRTLLVAGMHFQDAYNYDIERVKRCVIHYATP
                     DGKLYPFCTYNSGPCYREKIERQFSIPWDPKAGVRA"
     gene            complement(717..1058)
                     /locus_tag="VFC15_09105"
     CDS             complement(717..1058)
                     /locus_tag="VFC15_09105"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZM10353.1"
                     /translation="MDDCLFCKIIAGKIPAKKVYEDDRVFVFEDINPKAPTHVLIIPK
                     RHVVGLKEATPEDAEILGYCQLIAAKIGRERGIEDGYRTVYNVGPRSGQSVFHLHLHL
                     LGGRDLTWPPG"
     gene            1613..1933
                     /locus_tag="VFC15_09110"
     CDS             1613..1933
                     /locus_tag="VFC15_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZM10354.1"
                     /translation="MPARNEPTHITRGNVFLDLGFSPEKALALKFKAKILIAILDEVK
                     RKKYRQAQLVKVLDEHQPVVSNLLHGKITQMSIEKLLIYADRLGLSLDVQKPRPPARR
                     RRAA"
     gene            2129..2386
                     /locus_tag="VFC15_09115"
     CDS             2129..2386
                     /locus_tag="VFC15_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM10355.1"
                     /translation="MQTKISTKGQVVLPGAIRRRLGLQAGDPLDAKVEGERIVLTPQR
                     KRKTKGKIVIDPLTGFPALTLGPGAPILTSKEVEEILSDFP"
     gene            2383..2793
                     /locus_tag="VFC15_09120"
     CDS             2383..2793
                     /locus_tag="VFC15_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM10356.1"
                     /translation="MKYLLDVSALLAFGIIDHEFHERVAAWIRGLTSNAVLELATCSI
                     TELGFVRVVAQTPRYGFTAPEARALLLQIKARQAAIFMFIPDNHDISHIPGWVKFPKQ
                     ITDGHLAELARANGAVLATLDRGIPGAFLIPEDK"
     gene            complement(2843..>3307)
                     /locus_tag="VFC15_09125"
     CDS             complement(2843..>3307)
                     /locus_tag="VFC15_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HZM10357.1"
                     /translation="ASVYVSPEIDETIEININPDDIRTDTYRSGGKGGQHVNTTDSAV
                     RITHIATGIVVACQNERSQHKNRERAMSILRSRLYEYELEKKRAVTKGIEDSKLDIDF
                     GSQIRSYVLQPYRMVKDLRTRIEIGDVDRVLDGDLNELIRAYLQLRRKEQRE"
BASE COUNT          723 a          931 c          911 g          742 t
ORIGIN      
        1 gcggactggt tcccgctggc ctgcgtcaca ccgttctcca agctgaccgc cgcgctgcgc
       61 ggcaccggcg tgcccaccat ttcgtcgcac cctcattgct cgctcggaac ttactttttc
      121 gtggacgaaa agtcgaagaa agccgtacct atcacccaat ttgttgacgt gccgaacatg
      181 ctgcaggaca tggaagagtt gtcacgttca gcgggcaagg cgcgcttcaa gctctacagc
      241 aaggtgaagg cgtggagctc gctgcagaag cacttcaagc cgcagcttgc tccgccggga
      301 ttgacgttca cgaaattcct gcagacgttg caggggatga caaacaagaa actcggccgc
      361 gacggaaaag atggaacgtt cacttatcgc acgctgctgg tggccggcat gcacttccag
      421 gacgcttaca actacgacat cgaacgggtg aagcgctgcg tgattcacta cgcgacgccc
      481 gatggcaagc tgtatccgtt ctgcacttac aactcgggcc catgttatcg cgagaagatc
      541 gagcgccagt tctctatccc gtgggacccg aaagcagggg ttagggccta ggggttaggg
      601 attagtggct agttagatcg tcgccaaatc caagctcatg ggcagccgga gaggcaacaa
      661 tgcgaaaccg atacggtttg ccaatcccta atccctaatc cctagcccct gcttttctac
      721 cccggtggcc aggtcaggtc gcggccaccc agcaggtgca gatgcagatg gaacaccgac
      781 tgccctgacc gtggccccac gttgtacacg gtgcgatatc cgtcctcgat accgcgctca
      841 cggccgatct tcgcggcaat gagctggcaa tagccaagga tctcggcgtc ttccggcgtg
      901 gcttctttca atccgacaac gtgccgcttg ggaatgataa gcacatgtgt cggcgctttg
      961 gggttgatgt cttcgaagac gaacaccctg tcgtcctcgt acaccttctt cgcgggaatc
     1021 ttccccgcga tgatcttgca gaagaggcaa tcgtccatgg ctcgatgtta tccgacgaaa
     1081 aacatttcgg ccacggattt cgcggaggga cacgaatcga acaatccaaa gtcttgctgc
     1141 tccgggcgga ggcttacttt tgttcccgct gctgcggctt ttccagcagt tggtgtccta
     1201 taataaatct tttataatcc ttttaatcca tgcgaacctg cacaggtgaa ccagggaata
     1261 gacgggccag atctcgcggc aattcactgg gaaggagatt cccgcgaggt tttggccgga
     1321 tttccggagg aggttcgcgg tagcctcggc ttcgccttgt atgaactgca gcgtggcagg
     1381 ccgtcatcag ttgcaacgcg gcggatggaa tccatcggtt ctggcgttta tgaattgaag
     1441 gaagccgacg aaagaacctg gtaccgggtg atctacctct cgaaaattga cgacgtgatc
     1501 tacgttttac actctttcga gaaacagagc cggaaaactg accggcggga cctcaatatc
     1561 gcgaaggaac ggttgtccct cgtgatacaa cgaatacgcg accggaggaa acatgccagc
     1621 gcgcaatgaa cccacccaca ttacaagggg aaacgtcttt ctcgacttgg gtttctcgcc
     1681 cgaaaaggcg ctggccctga aattcaaagc gaagattctg attgcgattc tcgatgaagt
     1741 gaaacgcaag aaataccgcc aggcgcaatt ggtgaaggtc ctagatgagc accagccggt
     1801 tgtgagcaat ctgctgcacg gcaaaatcac ccagatgagt atcgagaagc tgttgatcta
     1861 tgccgatcgc ctgggcctgt ccttggacgt tcaaaagccg cgccctccgg cacgccgacg
     1921 gcgggccgcg tagcggatac aattgggact cccgggcgct ttggggttga tgtcttcgaa
     1981 ggcgaacacc ttgtcgtctt cgtacacctt cttcgaggga atcttccccg caacgattgc
     2041 agaagagttt agtctagtta atgacaagtc gcccgattcg ccaggacatg gaccgggagg
     2101 atggtttgca tggtaagatt gtcttaccat gcaaacgaag atctccacca aaggccaggt
     2161 tgtccttccc ggtgccatcc gccgccgact tggactccaa gcgggcgacc ctctcgatgc
     2221 taaggtcgag ggtgaacgta tcgttttaac acctcaaagg aaacgcaaaa ccaaaggaaa
     2281 aatcgtgatc gatccgctta ctgggttccc cgcgctgact ctgggcccgg gtgccccgat
     2341 cctgaccagt aaggaagtgg aagagatact ctccgatttc ccatgaaata tctcctcgat
     2401 gtgagtgcgc ttctggcgtt tggcattatt gatcacgagt ttcacgagcg agtcgccgcc
     2461 tggatacgcg gcctaacctc aaatgcggtt cttgagttgg ccacgtgttc gatcacggag
     2521 cttggctttg tacgcgtcgt ggcgcaaact ccacggtacg gattcactgc tcctgaggcg
     2581 cgtgccttac tactgcagat aaaggcacga caagcagcaa tattcatgtt cattcccgac
     2641 aatcacgata tttctcacat ccccggctgg gttaagtttc cgaagcaaat tacggacggt
     2701 cacttggccg aactcgccag agcgaacggt gccgttctgg caactctgga tcgcggaata
     2761 ccgggggcat tcctcatccc tgaagacaag tgaatccaga cacgtgtgtg gatcggtgtc
     2821 gattccatgg ttgcggactc ggctactccc tctgctcttt cctccttaac tggagatacg
     2881 cccggatcag ttcgttcaag tcgccgtcca gcacgcgatc cacatcgccg atctcgattc
     2941 tcgtccgcag gtccttcacc atgcgatagg gctgcagcac gtaggaccgg atctgcgagc
     3001 cgaagtcaat gtcgagcttg gaatcctcga tgcctttggt taccgcgcgc ttcttctcca
     3061 gttcgtactc gtagagccgc gaacgcagga tgctcatggc gcgttcgcgg ttcttgtgct
     3121 gcgaacgctc gttctggcag gcgaccacga ttcccgtcgc gatgtgtgta atccgcaccg
     3181 ccgaatccgt ggtgttaacg tgctgtccac ccttgccccc ggagcgatag gtgtcggtgc
     3241 gaatgtcatc cggattgatg ttgatttcga tggtttcgtc aatttccggc gagacataga
     3301 cgctggc
//
LOCUS       DASRKK010000403         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1106186, whole genome shotgun sequence.
ACCESSION   DASRKK010000403 DASRKK010000000
VERSION     DASRKK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106186"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFC15_09130"
     CDS             <1..235
                     /locus_tag="VFC15_09130"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM10358.1"
                     /translation="ETDIVMNAEILSWSRNKGLFAGISLAGSTLRSDDDANKNLYGKE
                     LSAKQIILEKAVKTPPAGKSLVNLLNKMSPKHM"
     gene            444..728
                     /locus_tag="VFC15_09135"
     CDS             444..728
                     /locus_tag="VFC15_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZM10359.1"
                     /translation="MVDLQTRRYVISGRVQGVGFRWFVEREAARIGVTGWVRNREDSE
                     VEVMATGTREQHSALRASLQEGPRASRVDHVSESAAPLLEAPSFRIEGVW"
     gene            732..1277
                     /locus_tag="VFC15_09140"
     CDS             732..1277
                     /locus_tag="VFC15_09140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZM10360.1"
                     /translation="MQPHKSHNCDDLKKLIREVPDFPKPGILFYDITTLLKDKLGFAR
                     LIDALTEHYIDKHIDLVMGIEARGFIFGPALAYRLNAGFVPVRKPKKLPAEVLQWKYE
                     LEYGYDTLEVHKDSIEPGQRIVICDDLLATGGTAKATAEMAKSLGAEICGLGFVVELD
                     GLKGRDKLKGYDVFSLLHYEM"
     gene            complement(1332..1721)
                     /locus_tag="VFC15_09145"
     CDS             complement(1332..1721)
                     /locus_tag="VFC15_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM10361.1"
                     /translation="MILVDTHVLVWLELQPGKLSRAATSAIRRAQVSGGVAISAISLV
                     ELAGLIYRGKVRLPGSFESSLQRFIRDVTVKPITLEIAVLTSHFPPDFPSDPMDRIIA
                     ATARAENLPLVTADERILRCPLLKTIW"
     gene            complement(1718..2044)
                     /locus_tag="VFC15_09150"
     CDS             complement(1718..2044)
                     /locus_tag="VFC15_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZM10362.1"
                     /translation="MASTTVTQKGKTVPRRKRAAKPNRAGEAREIAAGEFKAKCLSLI
                     DQVNETGQEIVITKRGKPCAKLIPLKPWRMESLIGRLVGIGEIVGDPDDLIKPVFPLE
                     DYDMLK"
     gene            complement(2168..2773)
                     /gene="purN"
                     /locus_tag="VFC15_09155"
     CDS             complement(2168..2773)
                     /gene="purN"
                     /locus_tag="VFC15_09155"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZM10363.1"
                     /translation="MNNLGILLSGRGSNFEAIADNVASGKLDANIAVVISNRADAGGI
                     ESARRRGLKALVIPSKGKPREEHDREVVAALREHRVDLVCLAGYMRLLSPWFVQQFPN
                     RILNIHPALLPAFPGLEAQKQAFDYGVKVSGCTVHFVDADLDHGAILVQKVVPVLDSD
                     DEHTLAARILEQEHLAYSEAIRIVLEGGHHIVGRRVIRTRE"
     gene            complement(3124..3312)
                     /locus_tag="VFC15_09160"
     CDS             complement(3124..3312)
                     /locus_tag="VFC15_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10364.1"
                     /translation="MAKVNKFELEDPVPVLDDEDAETLAAIDEGIRDAEAGRTVPREE
                     VRKLLPKWISASSTRKER"
     gene            3400..>3588
                     /locus_tag="VFC15_09165"
     CDS             3400..>3588
                     /locus_tag="VFC15_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10365.1"
                     /translation="MRLPPPSLYYRPIVVLPPNKEHPDRIVSAVPVDDDSSFELKLRP
                     QRLREFIGQNKVKDNLEVA"
BASE COUNT          731 a         1055 c         1038 g          764 t
ORIGIN      
        1 ggagaccgac atcgtcatga acgctgagat tctgtcgtgg tcgcgcaata aagggctttt
       61 tgccggaatt tcgctggcag gctcgactct gcgttccgat gacgatgcca acaagaacct
      121 ttacggaaaa gagctaagcg ctaaacagat catcctggag aaggcggtaa agactcctcc
      181 ggccggcaag tcgctggtca acttgctcaa caaaatgtca cccaagcaca tgtaaggcac
      241 tgctgagaat agttaccgta attggtcgac acgcggttct ggtacatccc ggagccgcgt
      301 ttccttcagc aaggaggacc ctcaatcggg caagtccgct cccaaaccaa ttacaatcgc
      361 ttttcgcagc aaagcggaca attgccgccg ttcatcgata gttgttgcct tactaattac
      421 taattactaa ttgcttcccg ctaatggttg acttgcaaac ccgccgctac gtcatctccg
      481 gacgcgttca gggcgtcggc ttccgctggt tcgtggaacg cgaagcggcg cggatcggcg
      541 tcaccggatg ggtgcgcaac cgcgaagata gcgaggtgga agtgatggca accggcacgc
      601 gcgagcagca cagcgctttg cgggccagtt tgcaggaggg gccgcgcgcc tcgcgggttg
      661 accacgtcag cgaatccgcc gcgccgctgc tggaagcgcc atcgttccgt atcgaaggag
      721 tctggtaacc catgcagccg cataagtcgc acaattgcga cgacctcaag aagctgattc
      781 gcgaagttcc cgacttcccc aaacccggca ttctcttcta cgacatcact acactgctca
      841 aagacaagct cggctttgcc cgcctcatcg atgcgctcac ggagcactac atcgacaagc
      901 atattgactt ggttatgggt atcgaggcgc gcggattcat cttcgggccg gcgctggcgt
      961 accgcctgaa cgcgggcttc gttccggtgc gcaagccgaa gaagctgcca gccgaagtcc
     1021 tgcagtggaa atacgagctg gaatacggct acgacacgct ggaggtccac aaggattcga
     1081 tcgagccggg ccagcgcatc gtcatctgcg acgatttgct ggccaccggc ggcaccgcaa
     1141 aggcgacggc ggaaatggcg aagtcgctgg gcgccgaaat ctgtggtctg ggtttcgtgg
     1201 tcgaactgga cggcctcaaa ggccgcgaca agctaaaggg ctacgacgtg ttctcgctac
     1261 tgcactacga gatgtagggg cggttctcag tggccagttg tcagctgtcc atcgagcgtc
     1321 gaccgccttc tttaccagat agttttgagc agcgggcagc gcaaaattcg ttcatcagcg
     1381 gttactagtg gcaggttttc ggcgcgggcg gttgcggcga tgatacggtc catgggatca
     1441 gagggaaagt ctggcgggaa gtgtgacgtc aggactgcaa tctccagtgt gatgggcttc
     1501 actgtcacat cgcgaatgaa ccgctgaagg ctcgattcaa agctgcccgg caggcgcact
     1561 ttgccgcgat agatgagtcc ggccagttca accagcgaaa ttgccgagat ggccacccca
     1621 ccggatactt gcgcgcggcg tatggcggat gttgccgcgc gcgacagttt ccccggctgg
     1681 agttccaacc agaccagcac atgcgtgtcg acgaggatca tttaagcata tcgtaatctt
     1741 ctaacggaaa cacgggcttg atgaggtcgt cgggatcccc gacgatttcc ccgatcccga
     1801 ctaatctgcc gatcaaggat tccatcctcc agggtttgag cggaatcagc ttggcgcatg
     1861 gcttcccacg cttggtaatt acgatctcct ggccggtttc attgacctgg tcaatgaggc
     1921 tgaggcactt ggccttgaat tctccggcag cgatttctct cgcctcacct gcacggttag
     1981 gcttcgctgc ccttttcctc cggggaacgg tctttccctt ttgagtgact gttgtcgaag
     2041 ccatattggt cgcctccaga tgaccatggt cattttaaca tggtcatctc agttttttta
     2101 gtttattgtc atcccgagca acgagtgcag cgagaaacca tgggactgcg gttctccaaa
     2161 cgcgacttca ctccctagtc ctaatcaccc tacgtcccac gatgtgatgc cccccctcca
     2221 gaactatgcg gatggcctcg ctgtacgcga ggtgctcttg ctccaggatg cgcgcggcca
     2281 gcgtgtgctc gtcatcgctg tcgagcacag gcaccacttt ctgcaccaga atggcgccgt
     2341 ggtcgaggtc cgcgtccaca aaatgcaccg tgcagcccga gaccttcaca ccgtagtcga
     2401 aggcctgctt ttgcgcctcc agtccgggaa acgccggcag cagcgccgga tgaatgttga
     2461 ggatgcggtt gggaaactgc tgcacgaacc acggcgacag caggcgcatg tatcccgcta
     2521 ggcagaccag atcgacccgg tgttcgcgca gcgcagcaac cacttcgcgg tcgtgctcct
     2581 cgcgcggctt gcccttcgac ggaatcacca gcgcctttag cccgcgccgc cgcgccgact
     2641 cgataccgcc cgcgtccgcc cggttggaga tgacgaccgc gatgttcgcg tcgagcttgc
     2701 ccgaggcgac gttgtcggcg atggcctcaa agttcgaccc gcgcccggag agcaggatgc
     2761 caaggttgtt catgagcgat attcggaatg atactgaggg tcactgtaat tgtcagtgag
     2821 ggggatattc gttcgctcca cgacaacccg gtgcctaaca tatctcacgt gaggtttgtg
     2881 gcggtttccg agctccatgc cggaagtgga gcacttcgat aagacgcttg tcttcgcgca
     2941 ctcggtaata gaccagaacg gggctgtgca gcaatttgtg tatggtagag cgttttccca
     3001 gagcgttgcc catgcgtgga aagcgagcga gcaagtctac gtggtcgagt agagaattgc
     3061 caaagcgaga cgctgcctcg gcgtcgtcct cggcaatatg gccgatgatt ccagccaaat
     3121 cgtctagcgc tctttgcgtg tactggaggc ggaaatccac ttgggcagga gctttcgcac
     3181 ttcctccctg ggcacggttc gcccggcctc agcgtcgcgg atgccttcgt cgatggcggc
     3241 gagggtttcg gcatcctcat cgtcgaggac gggaacggga tcttctagct cgaacttgtt
     3301 caccttcgcc ataggctgat gttagctccg atgagtccgc agatgcaaac ggatcgtgag
     3361 caaccagcac cgtacccttc gtgaccggag cgcatttgtt tgcgcctccc cccgccttcg
     3421 ctttactatc gccccatcgt ggttttaccc ccgaacaaag agcatcccga ccgcatcgtc
     3481 tcggccgtgc cggtggacga cgactcgtcc ttcgagctga agctgcgtcc gcagcggctg
     3541 cgcgagttta tcggacagaa caaagtcaag gacaacctcg aggtcgcc
//
LOCUS       DASRKK010000404         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_616718, whole genome shotgun sequence.
ACCESSION   DASRKK010000404 DASRKK010000000
VERSION     DASRKK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616718"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VFC15_09170"
     CDS             complement(<1..1824)
                     /locus_tag="VFC15_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM10366.1"
                     /translation="MVDTTISHYRIVEKLGGGGMGVVYKAEDTRLGRFVALKLLPDDV
                     ARDPQALERFRREGRAASALNHPNICTVHDIGEEDGRAFIAMEFMDGATLKHLVGDRP
                     LALEQLLRIAIDVADGLDAAHAKGIVHRDIKPANIFVTKRGHAKILDFGLVKVTEKAT
                     SESDAVDTLSVDSDAKHLTSAGALLGTVAYMSPEQVRTKELDARTDLFSFGSVLYEMA
                     TGRMPFDGESTGVVYAEILHHQPPPPSSLNSATPPILDDIIADCLQKDRDRRYQLASE
                     IRADLKQLKHEMIVQGVIRDEDEETFESSNTGTGEHSSRTRSRSRRASAIAQTVPANS
                     ARHFPWKVAIPAGLILLALVAGGLYYRSHQGKRLTEKDTIVLADFANSTGDPVFDDTL
                     KTALSVALNQSPFLNVLSDNKVLATLQLMARPTTTKLTPEVARELCLRAGSKAYIAGA
                     VASLGSEFVLGIKAVNCQSGDLLAQEQVTAAAKEKVLDALGNAASKLRGELGESLGTV
                     EKFNKPLVEATTSSLEALQALTQAQRIAAEKGDASSLPHYKRAVELDPDFAMAYADLG
                     IGYSNLGQTSLAIENSKKAFALRDRASQRERFYIEAMYYSDV"
     gene            2489..>2780
                     /locus_tag="VFC15_09175"
     CDS             2489..>2780
                     /locus_tag="VFC15_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10367.1"
                     /translation="MSVFLLAGSNAQQSGSSSGKTTPSKAPVRVNPRTTSPPQTIYYV
                     GKVVVDTGTEPPAPVAVLRVCNGTGRREAYTTSDGSFSFVVGDRTNSILPDAS"
BASE COUNT          544 a          798 c          807 g          631 t
ORIGIN      
        1 gacatcgctg tagtacatgg cttcgatgta gaagcgctca cgctggctgg cgcggtcgcg
       61 cagtgcaaat gccttcttgg aattctcgat tgccaggctg gtttgtccca ggttgctata
      121 gccaattccg aggtcggcgt aagccatggc gaagtcagga tccaactcga cggcgcgctt
      181 gtaatgcgga agggacgagg catcgccttt ttctgcagcg atcctttgcg cctgtgtcag
      241 cgcttgcaga gcttccaacg aggatgtcgt ggcctcaacg agcggtttgt tgaacttctc
      301 gaccgtgccc agcgactcgc ccagttcgcc gcgcaacttg gacgccgcat tccccagcgc
      361 gtccagcacc ttctccttgg ccgctgctgt aacctgctcc tgcgccagca ggtctccgct
      421 ctggcaatta actgccttga tccccagcac gaattcgctg cccaggctcg cgacggctcc
      481 ggcgatgtag gccttactgc ccgcccgcag gcatagctca cgagctacct cgggcgtgag
      541 tttcgtagtg gtcggacgag ccatcagttg caacgtagca agaactttgt tgtcggaaag
      601 cacattgagg aatggcgact ggttcaaggc aacactgagc gccgttttca atgtgtcgtc
      661 gaacaccgga tcccccgtgc tgttggcaaa atccgcgaga acaatggtgt ccttctcagt
      721 caggcgcttg ccctgatgcg agcgatagta aagaccacca gcgaccagtg cgagcagaat
      781 gagtccggca ggtatcgcga ctttccaggg gaaatgccgc gccgagttgg ccggaactgt
      841 ttgggcaatg gcggaggcac gccgactgcg tgatctggtc cgactggaat gctcgcctgt
      901 tccagtgttc gacgactcaa aggtctcctc atcttcgtct ctaatgacgc cctgcactat
      961 catctcgtgc ttcagttgct tgaggtcagc gcggatctcc gaggccaact ggtaacggcg
     1021 atcgcggtcc ttttgaagac aatcggcgat gatatcgtcc aatatcgggg gggtggcgga
     1081 attcaggcta gatggcggcg gtggctgatg gtgcaggatt tctgcgtaaa caacccccgt
     1141 actctcgccg tcgaacggca ttctgcccgt tgccatctca tacagcaccg atccaaagga
     1201 gaaaagatct gtgcgcgcat ccaactcctt cgtccgcacc tgctcgggtg acatgtaggc
     1261 caccgtgccc agcagtgccc ccgcactcgt caggtgcttc gcatccgaat cgacgctgag
     1321 tgtatcgacg gcatcgctct cggaagtggc tttttccgtc accttgacca gaccgaagtc
     1381 gagaatcttc gcatgaccac gcttggtgac gaagatgttt gcaggcttga tgtcgcggtg
     1441 gacgatgcct ttggcgtgcg ccgcatcgag tccgtcggcg acgtcgatgg caatgcgaag
     1501 cagttgttcc aatgcaagcg gacggtcgcc gaccaggtgc tttagggttg cgccatccat
     1561 gaactccatg gcgatgaagg cgcgaccgtc ctcctcgccg atgtcgtgga cggtgcagat
     1621 gttgggatga ttgagcgcgg aggcggcccg accttcgcgg cgaaaacgct ccagcgcttg
     1681 cggatcgcgg gcgacgtcat cgggaagcaa cttgagggca acgaatcggc ccaagcgggt
     1741 gtcttcggcc ttgtacacga ctcccattcc gccaccgccc agcttctcta cgatgcggta
     1801 gtgcgagata gtcgtatcga ccatgttccg gctgaaagtt ggaaaccgat gcttatcgta
     1861 aggctgatcg cgaccgtacg tcaatttaca agctgcttgc ggcctgcgaa gtcctggatc
     1921 gtaggcctac tagaagttgg cccctaatcc ggcttgtggg atgtgtagga ctaaggttcc
     1981 gaccggttcg tcagcgagcg ggaagtaatt tactcttcgt ttacccccac tccatggtgc
     2041 cgatgtgtct gttgggcttt aggtttcgat gtgaaggttc gagccgtccg acgccgtcag
     2101 cgccagttgc gtccaaccca agtttcaacc caatctggtt tcggcgacca cgtgctttca
     2161 agtacttccg ggcaaatggc aggtccccga gctattgtaa gttgaaaata cttaatccat
     2221 ccgctatggc atggcagagg tcgccggttc gatcccggcc aggtccacca gtttcgctgc
     2281 caggttgccg gttttgccga aagactgtta tctatagtaa agttctattt ccttacagga
     2341 aaattgattc cgcggtgagg gtctgcgcga tcacaacttc cgtgtcgttt gggagtaaga
     2401 ttttgccact cgtaatgttg gggcgccgag tcttacttgg gggtgcacaa tgcggaggga
     2461 cgtagaattc gtcgcgacag gtcttctcat gtctgtcttc ctgctggccg ggtcgaatgc
     2521 gcaacagagc ggctcaagca gcggcaagac aactccatcc aaggcacccg tgcgcgtaaa
     2581 ccctcgcacc actagtccac cccagacgat ttattacgtt ggaaaggtcg tagtggatac
     2641 cggcaccgag ccccctgcgc cggttgcagt tctgagagtc tgcaatggta ccggccgcag
     2701 ggaggcatac actaccagcg acggctcttt ctcattcgtg gtcggtgatc gaacaaacag
     2761 catcttgccc gacgcctccg
//
LOCUS       DASRKK010000405         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1203924, whole genome shotgun sequence.
ACCESSION   DASRKK010000405 DASRKK010000000
VERSION     DASRKK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203924"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VFC15_09180"
     CDS             complement(<1..1388)
                     /locus_tag="VFC15_09180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM10368.1"
                     /translation="MKKIILGGLLLVICVMMVITLVPGGLFGDYFGGSVTTAGVLAKV
                     GTEDISLQQVAQRARMIGRQQFRGNIPPSIMPFLLQRAADGMITQGALAYEADRMGLG
                     ASDKEVADYLHQGQFGQLFFPGGNFIGQQQYEDFVQNQFGLTVVQFESELKNQLAQQK
                     LLAAVSAAATVSDKDVAEQVKKQDTKVKFDYAVLTLDDVKKQIKVTDVELKAFYDQNK
                     QQYVNSIPEKRKSRYILIDTAKLAASVPVTQADLQAFYNQHQDEFRIPETVTVRHILI
                     KTPTPGPDGKVDPKGVEAAKAKAEDVQKQLKSGASFDELAKKYSDDPGSAPNGGLLPP
                     ITKGRTVPEFEQAAFTTPKGQTTDIIRTSYGFHIIRVEDKQNARVKPLDEVKAQIEPA
                     VKQQKSAATAQSLANSVQSLARSSGMEKAAADKGLQVTTTELLAQTEPLPGVGTAQEL
                     SNGLFAAKKNDPP"
     gene            1654..2679
                     /locus_tag="VFC15_09185"
     CDS             1654..2679
                     /locus_tag="VFC15_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM10369.1"
                     /translation="MSITLKQVRRRVLAAKRRVREITMIVKGVASTDHPVMAHIIPIR
                     RCNLACKYCNEYDDFSKPVPLETMKRRIDDLARLGTTIVTLSGGEPLLHPELDEIIAH
                     MRKYPIIVGMITNGYLLTAERIQRLNRAGLDHLQISIDNVMPDDVSKKSLKVLDKKLQ
                     LLAEHADFHVNINSVVGGGTRNPHDALVVGRRAVKLGLTSTIGIIHDGGGQLQPLSDE
                     ERDVFLKMKNLEKKNYSRLNWFQNNIAYGKPNQWRCRAGARYVYICEDGLVHYCSQQR
                     GYPGVPLEQYTVEDIRREFLTEKSCAPHCTVSCVHQVSYFDFWRAPQTLQAPQTPVGA
                     GQLVQID"
     gene            2966..3952
                     /locus_tag="VFC15_09190"
     CDS             2966..3952
                     /locus_tag="VFC15_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10370.1"
                     /translation="MAKKDKDKDAKNKKKKDKGKKDKSKKAAEPVVQQTYTPGQSYAD
                     GTPMDSVTYLEAKLILKPDRFTSVQAFRDFGKLVQKTAKRVGVGFIPDVEAGLRPQVR
                     EITFGDTADFRLYNNAFILRRRIAYVDGFPVGDPEIVFKFRHPDEKQATSLDVRPNIA
                     GKYRIKFKAEALPLKNEVGGYRILYSHNCQFGLSQMHDADKMSMATLVKVFPALGKLQ
                     KQSDEKLSLVNGGIVEEVLLPLGQLDFGKGLVGKCDIGLWRTRGEHKSLVGEFAFQVQ
                     FDKRESVGDKQKKLAAQFYISLQNDVADWLALGVTKTGMVYRLNGNEPQSHE"
     gene            3945..4556
                     /locus_tag="VFC15_09195"
     CDS             3945..4556
                     /locus_tag="VFC15_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM10371.1"
                     /translation="MSKAGAKPAMQTTAGATTAPVEVSVFQIFLEFLIIGATSFGGVV
                     PYLRESLVTKRHWVDDKEFVEMLSISQSLPGLNATNMAVLVGEKLAGVMGSIAGVIGM
                     CLPGAVLMFVVGIFYRIHGDHIWVTAALKGVAAAAVGLILSTVVSLSKKSLSHKFDFV
                     FIAITVIAVNRLHLGVPRTLIGVGILAILFHYPRKEQKASAKL"
     gene            4553..5080
                     /locus_tag="VFC15_09200"
     CDS             4553..5080
                     /locus_tag="VFC15_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM10372.1"
                     /translation="MNQIPALIRVFSYLSLLTVGGGMAAFPEMKILTVDVHQWLDFKQ
                     LIHLYSVGQMAPGPNMMMIVSIGQWAGGTLGAIAVLIAFFGPTAILAFLVAKGWRKLE
                     KWPWRTSIQQGLAPVSIGLLLAGCFSMAKGAITGTETAAISVAVLLILLQYKINPALL
                     VVASAIVGVLTFMPK"
     gene            5191..5883
                     /locus_tag="VFC15_09205"
     CDS             5191..5883
                     /locus_tag="VFC15_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZM10373.1"
                     /translation="MFALLKMVDPDIPVKVPLEVLGFGSVMLLIIVLIHGAGLDRILD
                     HYERRSEVYRLRFRHPYLATSIFAVTILLMLFLHVFEICVWGVALNRTGLITSVRDSM
                     YFSANTYTTIGYGLMILPYNWRELSPLMAISGLFTFAWTTGELFGIVDRQRRLVKDLA
                     AQRKTRTTAMNRVPTQATGQAQATNGHQEQPEVGLTRDERRALREEIETKLKQLHDAE
                     RAEVEALRRGES"
     gene            5936..6421
                     /locus_tag="VFC15_09210"
     CDS             5936..6421
                     /locus_tag="VFC15_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10374.1"
                     /translation="MKRTLTLQNVVFALVVLVLLAAVPFSLRYAYQHGGFYLFSRQFF
                     VDLPKRLKGPGRMRFIFQPAVAIFFGIRAGINDARTGRQPYMLGVLFDGQQRRALLGE
                     AFERLSVLIAMSILLDALSQFLILHAVFPGAALVVGPVLIALPYSLARALTNRIARRT
                     S"
     gene            complement(6496..>7163)
                     /locus_tag="VFC15_09215"
     CDS             complement(6496..>7163)
                     /locus_tag="VFC15_09215"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HZM10375.1"
                     /translation="YGAMNRWRKLPDGSELLLVGTDNQAFPIPLKKNASGQWYFDTAA
                     GKDEILARRIGRDEIVAIDVCAALADAQAEYLAQKHDGVKQYAQKFISDPGKQNGLYW
                     ESAEGQPRSPVGPLVAFATEEGYSIKSSAYQAFHGYFFRRLDKQGPDAKGGAKNYIVN
                     GKMTSGFAYVAYPATYNDTGVMTFLIDQDRVVYQKDLGKDTADQAKAMTEFNPDKSWT
                     AVQ"
BASE COUNT         1481 a         2039 c         2060 g         1583 t
ORIGIN      
        1 ggaggatcgt tcttcttggc agcgaacaga ccattcgaca attcctgcgc ggtcccgact
       61 ccgggaagcg gttcggtctg cgccagaagt tcggtggtgg tcacctgcag gcccttgtcg
      121 gcagcagcct tctccatacc agacgaacgc gccaagctct gcacggaatt ggcgagactc
      181 tgtgcggtcg ccgcggattt ctgctgcttg accgcgggct cgatttgcgc cttcacttcg
      241 tcaagcggct tgacgcgcgc gttctgcttg tcttcgacgc ggataatgtg gaacccgtag
      301 ctggtgcgga tgatgtcggt cgtctggcct ttgggcgtgg tgaaagccgc ttgctcgaat
      361 tccgggacgg tgcgtccctt ggtgataggc gggagcaggc ctccgttggg ggcgctgccc
      421 ggatcgtccg aatacttctt cgccagttcg tcgaaactcg caccagactt caactgcttt
      481 tggacatcct cggccttcgc cttcgcggct tccacgccct tgggatcgac cttgccgtct
      541 ggtcctggcg tcggagtctt gattagaatg tggcggacgg taaccgtttc ggggatgcga
      601 aattcgtcct gatgctggtt gtagaacgcc tgcaggtcgg cctgtgtcac cggaaccgaa
      661 gcggccagtt tggcagtatc aataaggatg taacgtgact tgcgcttctc cggaatggag
      721 ttcacatact gctgcttgtt ctggtcgtaa aacgctttga gctcgacgtc ggtgaccttg
      781 atctgcttct ttacgtcgtc gagggtcagg acggcgtaat caaacttgac cttggtatct
      841 tgtttcttaa cctgctctgc aacatctttg tcgcttacgg tcgcagccgc actcaccgcc
      901 gcgagcagtt tctgctgggc gagttggttc ttcagttctg attcgaactg aactacggtc
      961 agcccgaact ggttctgcac gaaatcttcg tattgctgct gtccgatgaa gttaccgccg
     1021 gggaaaaaca actggccgaa ctggccctgg tggaggtagt cggcaacttc cttgtcgctc
     1081 gcaccaagtc ccatgcggtc ggcttcgtac gccagggcac cctgggtgat catgccgtca
     1141 gctgcgcgct gcagcagaaa cggcataatg ctcggcggaa tgttgccccg gaactgctgg
     1201 cgcccgatca tgcgggcacg ttgggcaacc tgttgcaggc tgatgtcctc ggtgccgacc
     1261 ttggccagca cgccggcggt ggtcacactg ccgccaaaat agtcgccgaa aagtccgccg
     1321 ggaacgaggg taatgaccat catcacgcag atgacaagta ggaggccgcc caggatgatt
     1381 ttcttcattg ggccaggggt ttgcagaaat cgcagcatgc ttgaaagaaa ctccaggatt
     1441 ggttgatcga atgcttgatt ataaaccagt actggctagc gtgagatgtt ccgctatgca
     1501 agttccgcta caccatgaag ttaggtccga gggcgcgatt tcgcgaagcc atcgggtggc
     1561 ctagcggcac cactcggggg cgattattcc ccgaacaaag gcgggggctt ccctgagtcg
     1621 tagggcaatg tgttgtaatc aaaaggcaaa ggtatgtcga ttacgcttaa acaggttcgc
     1681 cggcgcgttc ttgccgcaaa acgccgggtc cgcgagatca caatgatcgt caagggagtg
     1741 gcctccaccg accaccccgt gatggcgcac atcatcccta ttcgacgctg taacctggcg
     1801 tgcaagtact gcaacgaata cgacgatttc tccaagccag tccccctcga aaccatgaag
     1861 cgccgcattg acgatctggc gcgattggga accaccatcg ttacgcttag cggtggcgag
     1921 cccttgttgc atcctgagtt ggacgagatc atcgctcaca tgcggaagta tccgatcatc
     1981 gtgggcatga tcacgaatgg atatttgctg acggcggagc ggatccagag gctgaaccgc
     2041 gcgggactcg accacctgca aatcagcatc gacaacgtga tgcctgacga tgtttccaaa
     2101 aagtcgctga aggtgctcga caagaaactg cagttgctgg cggagcatgc cgacttccac
     2161 gtgaacatta actccgtggt tgggggcggt acccgcaacc cgcacgatgc cctggtggtg
     2221 ggacggcgcg cggtgaaatt aggtcttacc tcgaccatcg gcattatcca cgatggtgga
     2281 ggccagttgc agcctctttc cgacgaagaa cgcgacgtct ttctcaagat gaagaatctt
     2341 gaaaagaaga attactcacg actgaactgg ttccagaaca acattgcgta cggcaagcct
     2401 aatcagtggc gatgccgcgc cggcgcccgt tacgtttaca tctgcgagga cgggctggtg
     2461 cactactgct cacaacagcg cggatatccc ggggtccctt tggagcagta cacggtggaa
     2521 gatattcggc gtgagtttct gacggagaag agctgcgcgc cacactgcac cgtatcgtgt
     2581 gttcaccaag tctcgtactt cgatttttgg cgggccccgc aaaccctgca ggcgccccaa
     2641 actccagtcg gcgccggcca actggtgcag atcgactagt tccgacgatt cagccggtta
     2701 gacctcgcaa gacccaactt ccgcggtcat cgccgtcctg taagcagcta ttctttgatg
     2761 gctattttcc tagtgacgta cccccctgcg aaagagtaca aagttaaagc tacttcacaa
     2821 atctgatgga acctgacaca ttgcccggcg acggttgggc cctggatgtt gatggtcgcc
     2881 tagagtttat gagagtccct cacggcagtg gtcatcctgc agcgcgatct tccatccgac
     2941 gacattgagc attttaggag atccgatggc aaagaaagac aaagataaag acgctaagaa
     3001 caagaagaag aaagacaagg gcaagaagga caagagcaaa aaagccgcgg agcccgtagt
     3061 ccagcagacc tatacgccgg gccagtccta cgccgacggc acgcccatgg atagcgtgac
     3121 ctacctcgaa gccaagctga tcctgaagcc cgaccggttc acgtcggtgc aagcgttccg
     3181 cgatttcggc aagctggtgc agaagacggc gaagagggtt ggcgtcggtt tcatccctga
     3241 cgtggaagcc gggctgaggc cgcaagtccg ggagatcact ttcggcgaca ccgctgattt
     3301 tcgcctgtac aacaatgcgt tcatcctgcg gcggcgcatc gcctatgtgg acggctttcc
     3361 cgtgggcgat cctgagatcg tgttcaagtt ccgccatccg gatgaaaagc aggcaacgtc
     3421 gctggacgtc aggccaaata ttgccggtaa ataccgcatc aaattcaaag ccgaagccct
     3481 tcccctgaag aacgaggtgg gcgggtaccg catcctttat tcgcacaact gccagttcgg
     3541 attgagccag atgcacgacg ccgacaagat gtccatggcc accctggtga aggtgttccc
     3601 cgcgctcggc aaattacaaa agcaatctga cgagaaacta tctctggtga atgggggcat
     3661 cgtggaagag gtgctgcttc cacttggaca actggacttc ggcaaagggc tggtcggcaa
     3721 gtgtgacatc gggttgtggc gcacccgcgg cgagcacaag tcgctggtgg gcgagttcgc
     3781 cttccaagtg cagttcgata agagagaaag cgtcggcgac aagcagaaga agctggccgc
     3841 gcagttctac atctccctgc aaaacgatgt ggcggattgg ctggcgctgg gcgtcaccaa
     3901 aaccggcatg gtgtaccgcc tcaacggtaa cgagccccaa agccatgagt aaggctggcg
     3961 cgaaacccgc gatgcaaacc acagccggtg ctacgacagc gccggttgaa gtctcggtct
     4021 tccagatctt cctcgagttc ttgatcatcg gagcgaccag cttcggtggg gtcgtgcctt
     4081 acctgcgcga aagccttgtc accaagcgac actgggttga cgacaaggaa ttcgtcgaga
     4141 tgctttccat cagccagagc cttccgggct tgaatgcgac caacatggcg gtgctggtgg
     4201 gggaaaaact ggctggcgtg atgggctcga tcgcaggtgt catcggcatg tgcctgccag
     4261 gggcggtgct catgtttgtg gtgggaatct tctaccgcat ccatggcgat cacatctggg
     4321 tcacggccgc gctgaaaggc gtggcagccg ccgcggttgg cctgatcctg tcaacggttg
     4381 tgagcctcag caagaagtca ctctcgcata agtttgactt cgtgttcatc gcgataacag
     4441 taattgccgt gaaccgttta catctgggag tgccacgtac tctgatcggt gtcgggattc
     4501 tggcgattct gttccactat ccgcgcaaag agcagaaagc gagtgcgaag ctatgaacca
     4561 gattccagcg ctgattcgcg tgttctctta cctgtcgctg ttgaccgtcg gcggcggcat
     4621 ggctgcgttt ccagagatga agatcctgac ggtcgatgtc caccagtggc tcgacttcaa
     4681 gcaactcatc catctttata gcgtggggca aatggccccc ggccccaaca tgatgatgat
     4741 cgtctccatc ggacaatggg cgggcgggac gctgggcgca atcgctgtac tgatcgcctt
     4801 tttcgggccg accgccatac tggccttcct cgtcgccaag ggatggagaa agctggagaa
     4861 atggccgtgg aggacttcca ttcagcaggg gctggcaccg gtgtcgatcg ggttactgct
     4921 ggccggctgt ttcagcatgg ccaaaggcgc catcactgga acggaaacgg cggcaatctc
     4981 ggttgctgtt ctgttgatcc tgctgcagta caagatcaac cctgctcttt tggtggtggc
     5041 cagcgccata gtcggggtgc ttaccttcat gccgaaataa tggtggataa tccgtgcgga
     5101 atacggccca cactcctcgc aaaccagcga agacctgggg gccgaagcaa ttggaactcc
     5161 gccgcatcac caacacggga taagcggtcc atgttcgctc tcctgaaaat ggtcgacccg
     5221 gacataccgg taaaggtccc gctcgaggta ttaggcttcg gcagcgtgat gctgttgatc
     5281 attgtcctga tacatggcgc cggactcgat cgtatcctcg accattacga acggagatct
     5341 gaggtctatc gccttcgatt tcggcatccg tacctggcga cgtcgatctt cgcagtgacg
     5401 atccttctca tgcttttcct gcacgtattc gagatttgcg tatggggagt tgcgctcaac
     5461 cgcacaggac tcattaccag cgtccgcgac tccatgtatt tcagcgcgaa cacctatacg
     5521 actatcggat acggtctgat gattctgcct tacaactggc gggaactcag ccccctcatg
     5581 gcaatctcgg gcctcttcac cttcgcctgg acgaccggcg agttgttcgg cattgtcgac
     5641 agacaacgcc ggctggtcaa agaccttgca gcgcagcgca aaactagaac aacagcgatg
     5701 aaccgcgtcc ccacgcaggc gactgggcaa gcgcaggcaa caaatggaca ccaggagcag
     5761 ccggaagttg gcctcacgcg agacgagcgc cgcgcacttc gggaagagat cgaaacgaaa
     5821 ctcaaacaac ttcacgacgc ggagcgggcc gaagtggaag cgcttcgccg tggcgagagt
     5881 tgactactat caacggccga gccgaaagag ttcgtgatgg gcaaaggtcc gtggcgtgaa
     5941 acgcactctt acactccaaa atgtagtttt cgctttggtc gtcttagtcc tgctcgccgc
     6001 agttccgttt tctttacgat atgcgtacca gcatgggggc ttttatctct tctcgcgcca
     6061 gtttttcgtg gaccttccta aacggctcaa gggcccgggc cgcatgcgat ttatcttcca
     6121 gcctgcagtc gctatctttt tcggcattcg agcgggaatc aacgacgcgc gcaccggacg
     6181 ccaaccgtat atgttggggg ttctcttcga tggtcaacaa cgacgagcat tgctcggaga
     6241 agcgttcgag cggctctcgg tgctgatcgc gatgtcgatc ctactggatg ctctatccca
     6301 attcctgatt ttgcatgcag tgttccccgg tgccgcattg gttgtcggcc cggttcttat
     6361 cgcgctgccc tattctctgg ctcgcgcgct gaccaaccga attgcgcgcc gcaccagttg
     6421 aggcaatccc tgttccgtaa gacgttcgcc ggagttcgag ctaagtcact gcgagagtcc
     6481 gcgcattagt tgcctctatt gcactgcagt ccaactcttg tcaggattaa actccgtcat
     6541 tgccttcgcc tgatcggcgg tgtctttgcc gaggtccttc tggtacacca cacggtcctg
     6601 gtcgatgagg aaggtcatca cgcccgtgtc gttgtaggtc gcgggatatg ccacataagc
     6661 gaaaccgctc gtcatctttc cgttgacgat gtagttcttt gcgccacctt tggcatccgg
     6721 gccctgctta tccagtcgcc ggaagaagta gccgtgaaac gcctggtacg cgctagactt
     6781 gatgctgtat ccctcttctg tcgcaaatgc caccagcggg ccgaccggac tgcgaggctg
     6841 cccctctgct gactcccagt acaagccgtt ctgtttgcct ggatcgctga taaacttctg
     6901 cgcgtactgt ttcacgccgt catgcttttg cgcgaggtac tcggcctggg cgtcggccag
     6961 cgccgcgcac acgtcaatcg ccacgatctc gtcccttccg atccgtcgcg ccaggatctc
     7021 gtctttgccc gccgccgtgt cgaagtacca ctggcccgac gcgttcttct tcaggggaat
     7081 aggaaacgcc tggttgtcgg tgcccaccag caacaactcg ctgccatcgg gcagcttgcg
     7141 ccagcggttc attgccccat acg
//
LOCUS       DASRKK010000406        22382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1008836, whole genome shotgun sequence.
ACCESSION   DASRKK010000406 DASRKK010000000
VERSION     DASRKK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22382
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008836"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2822)
                     /gene="nifJ"
                     /locus_tag="VFC15_09220"
     CDS             complement(<1..2822)
                     /gene="nifJ"
                     /locus_tag="VFC15_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZM10376.1"
                     /translation="MKTTETEEANSAASIEDANESQSRGGINTEEPAPIGDEAGKGKA
                     ARNKVTLDGNEAVAHVAYHVSEVAAIYPITPSSPMGESSDAWASIGEKNIWGTVPKVV
                     EMQSEGGAAGALHGALQAGALGTTFTSSQGLLLMIPNMYKIAGELTSTVIHVAARAVA
                     AQALSIFGDHQDVMATRQTGFALLSSNSVQEAMDLALVAHAASLESRIPFLHFFDGFR
                     TSHEVSKIEELSLDDMRAMIDDDLVRAHRSRALSPDHPVLRGSAQNPDVYFQGREAVN
                     PYYIACATIVQNAMDKFAAIVGRQYHLFEYVGAADAENVIIIMGSGAEAVHEAVDYMT
                     AQGKKVGVLKVRLVRPFSVEHFLDALPKTTKSIAVLDRTKEPGSIGEPLYQDIVTALT
                     EGFTAGKCKLAAFPKVVGGRYGLSSKEFTPAMVVRIYDELTKATPQNHFTIGIVDDVT
                     HTSLDYDSTFSTESPDTVRAMFFGLGADGTVGANKNTIKIIGEDTNLFVQGYFVYDSK
                     KSGSVTTSHLRFSKKPIHSTYLISSANFVGCHQFSFMERLDVLRAADPGSVFLLNSPF
                     GPDDVWDNLPRSAQKTIIDKKLKFYVIDAVEVARAAGMGGRINTVMQTCFFAISGVLP
                     RDEAVASIKHSIEKTYGKRGESVVRKNFAAVDASLENLHEVKVPAAISSKFDLRPTVP
                     ANAPEFVRSVEGLMIAGDGDLIPVSAMPIDGTFPTGTTQWEKRNIALEIPVWDEALCI
                     QCGKCVMVCPHAVIRAKVYDPAAINHAPEGFQTAAPKWREFSDLKYTLQVAPEDCTGC
                     GLCVEVCPAKSKSEAKHRAINMEAQPPLREKYNDYWSYFLSLPELSREKLAHGQVKDA
                     QLLEPLFEFSGACSGCGETPYIKVMTQMFGDRALIANATGCSSIYGGNLPTTPYTKNK
                     EGRGPAWSNSLFEDNAEFGMGMRIAV"
     gene            3066..3761
                     /locus_tag="VFC15_09225"
     CDS             3066..3761
                     /locus_tag="VFC15_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM10377.1"
                     /translation="MGKGAPLRWIENKLLARHLRGTGIEIGALWRKFPVRPSATVWYV
                     DRVHPDALQQDYPEVGQKLIFPDVVADAVQLPFPDASLDFVLASHVLEHMPFPLATLR
                     HWYGALAPGGILLLKVPDKRYTFDARRQRTPLQHLIAEDQDPSLFDKRAHFEDWVEYV
                     VGLARGTEEFDRETDRLMQIDYSIHYHVWTDEDVRALIDYSRGSMKLSWRPILFFNAH
                     FYRKECIVLVQRN"
     gene            complement(3770..4807)
                     /locus_tag="VFC15_09230"
     CDS             complement(3770..4807)
                     /locus_tag="VFC15_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZM10378.1"
                     /translation="MKYYAILLVSFGGPECNADVLPFLENVLRGRNVPRERMLQVAEH
                     YYLFGGKSPINDQNRALIAALSAELKGHGPDLPIYWGNRNWHPMLADTLRQMKADGVK
                     RAIAFATSAYSSYSSCRQYRENIAAAQAAAGDGAPQVDKLRVFYNHPLFVEANVDHVH
                     EALNRIPEDRRASARIAYTAHSIPLGMSQSCDYQRQLLEVGRLVSEALERTPDPLVFQ
                     SRSGPASQPWLEPDILDHLREIKQQGVTDVVIAPIGFVSDHMEVLYDLDTEARLLCAE
                     LGLNMERAATAGTHPKFVKMIRELIVERMDESSAKQVVGIYPANPDVCLVDCCPVPVR
                     PATPTVATTAV"
     gene            complement(4818..5363)
                     /locus_tag="VFC15_09235"
     CDS             complement(4818..5363)
                     /locus_tag="VFC15_09235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM10379.1"
                     /translation="MMKQIGLVGAVGLLTLMIVSTAFDGSVPSVGSAAPNFTLNSQDG
                     NPITLSQFRGKWVVLYFYPRDMTKGCTIEAHNFQRDQPKFQAANAVVLGVSVDDTKSH
                     QEFCTKEGLTFKLLSDTDHKVSEEYGSLTNLGLVKYSKRYTFIINPEGKIVQVYTDVD
                     ADINQHSEQVLAALAQLQKKG"
     gene            5674..6075
                     /locus_tag="VFC15_09240"
     CDS             5674..6075
                     /locus_tag="VFC15_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM10380.1"
                     /translation="MKIARLLPAFSVLFLLIAFCSAYAQGPPQGSILLKSPSMGAVKF
                     PHSAHLKVAGRCEVCHHASKPAKSLKSPQEACTDCHTKPATPPVTIGLQAAFHNPTAT
                     AGLCVTCHQTENSHGKAAPVKCADCHKKENS"
     gene            complement(6119..6802)
                     /locus_tag="VFC15_09245"
     CDS             complement(6119..6802)
                     /locus_tag="VFC15_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZM10381.1"
                     /translation="MYAIVRNILLVYAALLPIVNPLGMAPIFAGMLESYDLSVRRTVA
                     MRVAVNGIVLLMASLFIGSYVLDFFGVSIGAVQLGGGLVVVVAGWRILNQQSNLSERH
                     VPEQPSSETILSQAFYPLTMPLTVGPGSIAVALTLGSNAHEESHMQLLESALISTIGI
                     VLIGITIYLCYRFSDALERFLGANGTSIVVRLSAFILVCIGIQIMFNGADEFYTEMVK
                     NAKIALEPH"
     gene            6918..8291
                     /gene="nhaA"
                     /locus_tag="VFC15_09250"
     CDS             6918..8291
                     /gene="nhaA"
                     /locus_tag="VFC15_09250"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZM10382.1"
                     /translation="MTDKKGIPARTYGALQIQLRESLFTRTLVLPIQEFIHIQGMSSI
                     LLLGAAIAALIWANSPWHDSYHHVWELQLTLSSIQLPVHAWINDALMAIFFFLVGMEI
                     KHEIVHGELSDIRKAALPIVGAVGGMVLPALLFVAVNFRGPGQHGWGVPMATDIAFSL
                     GVLGMVKGIPSELKVFLLTLAIADDIGAIIVIAFFYTDTLHVQALFVGLFFLLVMFGL
                     RRIGISRAIVFVVLGVAFWVAILQSGVHATIAGVILGFMVPTTPRLSLKEFQEIGVDM
                     IHRFRAARAEGDLATANRLLGSFEQLVNATEADSERLTRKLNDWVSFLVLPLFALSNA
                     GVTFAGGAVKELTASRIVWGVLLGLVIGKPLGIVGFCKLAVKLGLARLPNGVTWLQMA
                     AVGTLAGIGFTVSIFISSLAFDEPSHLMEAKTAVLAASLIAGLAGFFALRHEAKLEGA
                     AEVRVED"
     gene            8508..9299
                     /locus_tag="VFC15_09255"
     CDS             8508..9299
                     /locus_tag="VFC15_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10383.1"
                     /translation="MRSRFLTVMLGAILAMGLAACSKKEQPAQTPPATTDQSAQPTPA
                     ANPQTQAAPGQSAAAPAASPTGAPGEAPAAAPAAAPVAEQAPPPPPPEPIVIPAGKTI
                     TVRMGEGLSSKTAQDGQTFTGTLANGIAVNGKVVIPAGSGVTGTVSDAKSAGRFKGEA
                     IIAIRLTSINVKGAPHNVGTDEYCVTQKGKGKRTAVMVGGGAGGGALIGGIAGGGKGA
                     LIGGLVGAGAGTAGAAFTGNKELTIPAESAVSFKTTTAITIQPQS"
     gene            9413..10741
                     /locus_tag="VFC15_09260"
     CDS             9413..10741
                     /locus_tag="VFC15_09260"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZM10384.1"
                     /translation="MSKSLPLTIRHAMYNLRGGFLIRPLTIALALGCAGGFLSWLEEE
                     FPAVSSWVPSTLFPSHADPQVAQIILADIAGSIMTVVSIVFAILLMTLTLASMQFSPR
                     IIVSFSRDRVTQWTLGIFLGTFSYCMAALPAARSLPHPFAPVATVLGAMVLALVCVGL
                     LLFFIHHISQAISVNHIVDRIAEETEAMIDEMMPRPHHPHRMNDTEPLRANSTEIAVP
                     SHESGYIRFIDTRRLVAFAKHHHVSVRVLRRVGQFVPAGIPLMMVSKGNRLPAGASDE
                     LLAAFDIGPTRTLQQDVEFGVLQIVDVALKAISPAVNDPTTAITCVDQLSRILIRFAS
                     RETPDELLYDPPGVVRASIRWMHFERLLEAAFEQIRMYSKADVAVSLRLLRALSDIAT
                     STLDPEYRRVLIEQGIRIVTGCEEKLGEEELSPLRARQATLENLLSIEQA"
     gene            complement(10780..12237)
                     /gene="glmU"
                     /locus_tag="VFC15_09265"
     CDS             complement(10780..12237)
                     /gene="glmU"
                     /locus_tag="VFC15_09265"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZM10385.1"
                     /translation="MMNPQSGSAHSAGQSKFDFAIAILAAGKGTRLKSRHPKVLHQIA
                     GKTLLGHVVAAASKIVPPENIFAIIGHEAELVREALQCTGIKFVLQREQRGTGHALMA
                     AREALQGFGHVLVLSGDVPLIHPETIQRVRDFHIANQAAMTVLTAEPADPTGYGRVFR
                     KQKGGEPTDEVDRIVEQKALRNNEGEQREINSGIYAFAIAPLYTHIDELTTDNPAGEF
                     YLTDMAGVLGKSGNEVVALRAKDSNEVLGVNTRMELAELDAKLRAKKARQLMESGTTI
                     FRPETCDIDADVEIGTDTVIEPFVQIIGQTKIGSDCRIRSYSVITNCEIGDKVLIRPG
                     CIMESSVVRDGAQIGPYSHLRSGSDVGEGAHVGNFVETKKTKLGKRSKANHLTYLGDA
                     VIGEGVNVGAGTITCNYDGVNKHETVIEDGVFVGSDSTLVAPVKIGKGAYIGAGSCIT
                     EDVPSDALALSRSRQVNKSNWAAEKRAKIKSAKQT"
     gene            complement(12263..13369)
                     /gene="yvcK"
                     /locus_tag="VFC15_09270"
     CDS             complement(12263..13369)
                     /gene="yvcK"
                     /locus_tag="VFC15_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZM10386.1"
                     /translation="MAFLAISDLAMQSSNHSSEVRPLKVVAMGGGTGLSTLLHGLKQH
                     VILPGEEAFDRLLPFISDLSAIVTVSDDGGSSGRLRKELNVPPPGDIRNCIVALAEKE
                     ALLSRLFQYRFKAGEGLEGHSFGNLFLSAMTDITGDFSEAVRYTASILATRGHIYPAT
                     TSNAQLYAIMDDGSVVRGETNITASRRRITELRMAPANAKPARAALEAIESADLITIG
                     PGSLFTSLVPNLLVRGLSAAIKNSGATKVYVCNLMTQANESLNLTASQHIQAIYNHAR
                     HEFFDYALVNHTAASPTLAAKYAEKQQSQIVVDAEQIEALGVKPILGDYLTEDFDPNE
                     GLIARHDTHHVAHDLLRLLLDTREAPVAATKHKS"
     gene            complement(13357..14007)
                     /locus_tag="VFC15_09275"
     CDS             complement(13357..14007)
                     /locus_tag="VFC15_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10387.1"
                     /translation="MKKKEAENVQETKGKTKKQPDLLASSTGSEIRARSAKPLKVADD
                     PRFSQAVQNYEAGLKALQSHKYDKAKACFEKLVGGSSPELADRALVHLNTCNQQLNRD
                     PAKFKTPEEQYDYAVSLMNMGDYIRAREVFSDLSQQHPKLDFVWYGAAALNCLMGHFP
                     DAIAGLNEAIRLNPANRFQARNDSDFKSLSDDPRFTELLYPDTSAEVPSDSPKWHF"
     gene            complement(14151..14558)
                     /locus_tag="VFC15_09280"
     CDS             complement(14151..14558)
                     /locus_tag="VFC15_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10388.1"
                     /translation="MIVVLAVMAFAQGDSVPAYNLRPPLAGELLPILPADQRVGEVFR
                     YPYQVHAYDLAAKIPGVIHQLPCYCYCERIGHKSLHTCFESNHGAHCGICMKEVYYAF
                     QETKLKKTPAQIRAGIIKGEWKSIDLEAAANMN"
     gene            14731..15327
                     /gene="lepB"
                     /locus_tag="VFC15_09285"
     CDS             14731..15327
                     /gene="lepB"
                     /locus_tag="VFC15_09285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZM10389.1"
                     /translation="MPTDSNMPSSIGTHPQPLGLDERTARGRIPPVFARWVRDILISL
                     AVSAFFIIFLYQPVKVEGTSMMPTLEDQERVFINKFVYRLEAIERGDVVVFRYPRDTS
                     KSYIKRVIAVAGDRVKIDDGLVYVNDRQIAEGYVPQVYEDVRSYPETVVPPHSYFVMG
                     DHRNLSNDSRDFGSVDESYIYGKAVFGYWPFAKLGKLR"
     gene            15473..15925
                     /locus_tag="VFC15_09290"
     CDS             15473..15925
                     /locus_tag="VFC15_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM10390.1"
                     /translation="MRATLQLLLSIFVLSAMSFAADRDPIVLMQAERDFAKATADRGV
                     DGWMEFMAPNAVELSAEPLVGLDQIRAGMTKQFKLPGFKITWEPTHAEFLGQGDIGYA
                     VGRYEVRFTSEDGKPQFEKGSYLTTWQKQKDGSWKVVSDIGSPDPPAH"
     gene            15998..17131
                     /gene="carA"
                     /locus_tag="VFC15_09295"
     CDS             15998..17131
                     /gene="carA"
                     /locus_tag="VFC15_09295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZM10391.1"
                     /translation="MQAILALEDGRIFHGKGFGAKGECYGEVVFNTSITGYQEIFTDP
                     SYAGQIVVLTNPQIGNYGTNSEDNEATRPYIEGLIVREFSRVSSNWRSQEVAEEYLER
                     YKIPVLAEIDTRALVRHLRTYGVMRGVISTIETDTDKLVAKARSIPKMDGTDLAKEVT
                     TPRVYQWEEGVRSFEPTEVVGVKDDAPKYKVVAYDYGIKHNILRRLVGESCSVTVVPA
                     TTSAEDVLAMKPDGVFLSNGPGDPEPVTYAQASIRRMMGKVPIFGICLGHQLIGLALG
                     GKTYKLRFGHHGGNHPVKQMATGKIEITAHNHNFAVDPDSLKQSEVDLTHIDLNDNTL
                     EGLRHRSLPLFSVQYHPEAAPGPHDSQYLFKDFVKMMEEWKGK"
     gene            17163..18338
                     /locus_tag="VFC15_09300"
     CDS             17163..18338
                     /locus_tag="VFC15_09300"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZM10392.1"
                     /translation="MPRKKSSVSGIFLSHSSRDKEFVSRLASDLVNRGFPVWFDSWEM
                     EMGDSLYSKIYEGIDSSSVLILVMSKNAVKSDWVKKELHGALLKEEELKNRFLLPIKP
                     EECKVPFSIRDRIYADFSTDYLMQLERLEKVLRARGADKIDVAFQKELVPLRISRGLY
                     LDEISFQSRISALVPRIKAGNQIEVSQLVLYPEPEYGDARRLATRRINELDKSQNHTP
                     EAEVDLRSTYESIRRLEDALLVGVAGIMNGLVKMNEWAFVGLACHWYVRIIRSMLLFR
                     IWRLQSELSGATEFGKDALADPVNNDTAARFFGIDDVICCDVWKPPSNEYYSFFVDAD
                     SEVGRWFQEHPQCPDLLRAFWSPTLMYKYAIPQMVYKSYTNPAPFGWDFADCKIGRH"
     gene            18345..21635
                     /gene="carB"
                     /locus_tag="VFC15_09305"
     CDS             18345..21635
                     /gene="carB"
                     /locus_tag="VFC15_09305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZM10393.1"
                     /translation="MPKRNDIAKILILGSGPIVIGQSAEFDYSGAQACKALKFEGYEV
                     VLVNSNPATIMTDPEMADRTYIEPLNRTYLEEIIRVEAEMLAESGRPGIFAVLPTVGG
                     QTALNLAVELADTGVLEKYGAQLIGAQLDAIKKGEDRLLFKDAMTKIGLEVPKSSLVN
                     NLKDGMDFTSKIGFPVILRPSFTLGGTGGGIAYNREELLELLARGLDLSPVHEVLLEE
                     SVLGWKEYELELMRDLADNVIVICSIENFDPMGVHTGDSITVAPAQTLTDREYQAMRD
                     AAIAVIREIGVETGGSNIQFAVDPHNGRMVVIEMNPRVSRSSALASKATGFPIAKIAA
                     KLAVGYTLDEITNDITRKTPACFEPTLDYVVVKIPKWQFEKFHGADESLGPQMKSVGE
                     VMAIGRTFKQALLKAVRSLETGKRIGSEKIEPRILTKRLVTPHPERLQYIRYALSQGM
                     TVREIAQMTNIDPWFLHQLKEISDEQAKLSSLPLDTIGTAALRHLKRVGFSDARLGNS
                     LQTNEGKVSAEKIRQLRKQHGVRPVYKRVDTCAAEFESFTPYMYSTYEDEDEATPTDR
                     KKVIILGSGPNRIGQGIEFDYCCCHAAFALRDDGYETIMVNCNPETVSTDYDTSDRLY
                     FEPLTYEDVMAVYEHEATGGAPVGVIVQFGGQTPLNLALPLKGAGVPIIGTSPESIDL
                     AEDRKRFGKLLEELDIPQPPGATAASVEEALANANRVGYPVLVRPSYVLGGRAMVIAY
                     DDESVHRYMKEAVEYSQERPVLIDHFLESATEVDVDALSDGEDVVIGGIMQHIEEAGI
                     HSGDSSCVLPSVDLSPDVLATIRDYTFRLARALQVIGLMNIQYAIQRDKVFVIEVNPR
                     ASRTVPYVSKATGVPLAKIAARLMTGRKLREFLPENVERGTDLDTGSFYYVKSPVFPW
                     GKFPGVDTVLGPEMKSTGEVMGVADSFGEAFAKAQAAAGQVLPTSGRVFISANDRDKP
                     FVAEVAQRFVELGFSLVATHGTAKILEDAGLQVERVYKVKEGRPNVVDLIKGERLHLI
                     VNTPHGIDPWFDEKAIRRAAVTARIPTITTLSAARAACEGIAALQRGDIKVYALQQLH
                     AERAAAAK"
     gene            21648..>22382
                     /locus_tag="VFC15_09310"
     CDS             21648..>22382
                     /locus_tag="VFC15_09310"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZM10394.1"
                     /translation="MFLHGISPPITTPFYTDGNIYFRKLEQNVEHYSRTPTAGIVVLG
                     STGEAILLSDIERREVLKTARDAAANDKVLIAGTGVESAIETLRLTEFAYDVGYDVAM
                     VRTPHYYKGQMANPQNILAFYRFVCDRSPLPVIIYNFPQATGYDIPAELVIELAEHPN
                     LIGIKESSGSIEKVHKMVEGTRHIQCKATVTETFAAVTGRMLQAAATSPSTEGGALVS
                     IGAPASSAASDKTPAPKPSSSAVKVVG"
BASE COUNT         4595 a         6665 c         6334 g         4788 t
ORIGIN      
        1 tcaaccgcga tacgcatgcc catgccaaac tcggcgttgt cttcaaacaa cgagttcgac
       61 caggcaggcc cacggccttc tttgttcttg gtatacggcg ttgtcggcag gttgccgccg
      121 tagatcgacg agcaaccggt agcgttggca atcaacgcgc ggtcgccgaa catctgcgtc
      181 atcaccttga tgtacggggt ctcgccgcaa cctgagcagg cgccggagaa ctcaaacagt
      241 ggctccagca actgtgcatc tttcacctgc ccgtgtgcca gtttctcgcg tgacagttca
      301 ggcagcgaca gaaagtagct ccagtaatcg ttgtactttt cgcgcagcgg aggctgggct
      361 tccatgttga tcgcccgatg cttggcttcg ctcttgctct tggccgggca aacctcaacg
      421 cagagaccgc aaccggtgca atcttccgga gccacctgca gtgtgtactt caggtcggag
      481 aactcgcgcc acttgggggc tgccgtctgg aagccttcag gagcgtgatt gatcgccgcc
      541 ggatcgtaaa ccttggcccg gatcacagca tgcgggcaaa ccatgacgca cttgccgcac
      601 tgaatgcata gtgcttcgtc ccacaccgga atttccagtg cgatgttgcg cttctcccac
      661 tgcgtggtgc cggtggggaa ggtgccatcg atcggcatcg cgctgaccgg aatgaggtcg
      721 ccgtcgccgg cgatcatcag gccttcgacg ctgcgcacaa actctggggc attcgcagga
      781 accgtcgggc gaaggtcgaa ctttgatgaa atggcggccg ggacctttac ctcatgcagg
      841 ttttccagcg aggcatcgac tgccgcaaag ttcttgcgga ccaccgactc tccgcgcttg
      901 ccatacgtct tctcgatcga atgcttgatg ctggcaacgg cctcatcccg cggcagcacg
      961 ccgctaatgg cgaagaaaca ggtctgcatg acggtgttga tgcgtccgcc catgcccgca
     1021 gcacgcgcga cttccacggc atcgatcacg taaaacttca gctttttgtc gatgatggtc
     1081 ttctgcgcgg aacggggaag attgtcccac acatcgtcag gtccgaacgg gctattcaag
     1141 agaaataccg aacccggatc ggcagctcta agtacgtcga gccgctccat gaagctgaat
     1201 tgatggcagc ccacaaagtt tgccgacgag atgagatagg tcgagtggat tggcttcttg
     1261 ctgaagcgca ggtgcgaggt cgtgaccgag cccgatttct tcgagtcgta aacgaagtag
     1321 ccctgcacga agaggttggt gtcttcgccg atgatcttaa tggtgttctt gttggcgcca
     1381 accgtgccgt ccgcgcccag tccgaagaac atggcgcgca ccgtgtccgg cgattcggtg
     1441 gagaacgtcg aatcgtagtc caggctggtg tgggtcacat cgtcaacgat gccaatggtg
     1501 aagtggttct gcggagtcgc tttggtcagc tcgtcgtaaa ttcgtacgac catcgcggga
     1561 gtaaattcct tcgacgacag cccgtatcgc ccgccgacaa ccttggggaa cgcagccagt
     1621 ttgcatttgc cggccgtaaa gccttccgtc agcgcggtga cgatgtcctg atacagcggc
     1681 tctccgatgc tgcccggctc cttggtgcga tccagaaccg caatgctctt cgtggtcttg
     1741 ggcagcgcgt caaggaaatg ctcgaccgag aacggacgca ccaggcgaac tttcagcacg
     1801 cccactttct tgccttgcgc ggtcatataa tccactgctt catggaccgc ttccgcaccc
     1861 gaacccatga tgatgatcac gttttcggca tcggccgcgc ctacgtactc gaacaggtgg
     1921 tattgacggc cgacgattgc agcgaacttg tccatcgcgt tctgcacgat cgtggcacag
     1981 gcgatgtagt agggattgac cgcttcgcgg ccctggaagt aaacgtcagg gttttgcgcc
     2041 gatccgcgca ataccggatg gtcgggcgac aaggcgcggc tgcggtgcgc gcgcaccagg
     2101 tcgtcgtcga tcatggcgcg catgtcgtcg aggctcagct cctcgatctt ggaaacttca
     2161 tgcgaagtgc ggaagccatc aaagaagtgc aggaagggaa tacgcgattc cagcgaagca
     2221 gcgtgcgcga ccagcgccag gtccatggct tcctggaccg agttcgacga cagcagcgcg
     2281 aagccggtct ggcgggtggc catcacgtcc tggtgatcgc cgaagatcga cagtgcctgt
     2341 gcggccacgg cgcgagcagc cacgtgaatc accgtgctag tgagctcgcc ggcgatcttg
     2401 tacatgttgg ggatcatcag cagcagaccc tgcgatgatg taaaggtcgt gcccagcgcg
     2461 ccagcctgca gcgctccatg cagtgcgccg gcggctccgc cttcgctctg catctcgacg
     2521 accttcggaa ccgtgcccca aatgttcttc tcgccgatcg atgcccacgc gtcggaagat
     2581 tctcccatgg gagacgaggg cgtaatcggg tagatggcgg cgacttcgct gacgtggtac
     2641 gccacgtgcg cgacggcttc attgccatca agggtcactt tgtttcttgc ggccttgcct
     2701 ttgcctgctt cgtcaccgat cggcgcgggc tcttcggtgt tgattccacc gcgcgattgg
     2761 gactcattcg cgtcctcgat cgacgcagcg gaatttgctt cttcagtttc agttgttttc
     2821 acgtctgttg tcccccagat cgagcttggt gcgcggtctg tcattggtta gaaaaccgtt
     2881 aatgttcaag atatcgggcg actgcccagc tagctgtgac cggggtacta actagcctgt
     2941 gacgcaggtc acatacttat gggcgacgag catgggcgac gcggctcggt gcgggtcatg
     3001 acgatctatc cgagccgggg actcgccgag cgcaactaac gcattccaaa ggctatagtg
     3061 ttggtatggg gaagggcgct ccgctccgct ggattgagaa caagctgctg gctcgacacc
     3121 tgcgaggaac aggcatcgaa atcggggccc tttggcggaa gttcccggtg cggccgtccg
     3181 ccaccgtctg gtacgttgac cgcgtccacc ctgatgcgct gcagcaagac tacccagagg
     3241 ttggacagaa gctcatcttc cccgatgtcg ttgcggacgc cgttcagttg ccgttcccag
     3301 acgccagtct tgattttgtt ctcgccagcc atgttctcga gcacatgccg tttcccttgg
     3361 cgacactgcg gcactggtac ggcgcactcg ctcccggcgg cattctactg ctgaaggttc
     3421 ccgacaagag gtacaccttt gatgcgcggc gacagcgcac cccgttgcaa cacctcatcg
     3481 ccgaagatca ggacccctcg ctgttcgata agcgcgcgca cttcgaggac tgggtcgagt
     3541 atgttgtggg actcgcgcgt ggcaccgagg agtttgatcg cgagacggat cgactcatgc
     3601 agatcgatta cagcattcac tatcacgtgt ggacggatga agatgtccgt gcgctgatcg
     3661 actactcccg tgggagcatg aagttgagtt ggcggccgat actattcttc aacgcgcatt
     3721 tctaccgcaa ggaatgcatc gtcttagtgc agagaaattg agaggggtgt tacaccgcgg
     3781 tggttgctac cgtaggagtt gcgggcctca ccggcaccgg acagcagtca accaaacaca
     3841 catccggatt cgcggggtaa atgcctacca cctgctttgc cgagctctcg tccatcctct
     3901 ccacgatcaa ctcgcggatc atcttcacga attttggatg ggtgccggcc gtcgccgcgc
     3961 gttccatgtt caggcccaac tccgcgcaga gaagtcgcgc ttcggtatcc aaatcgtaga
     4021 gcacttccat gtgatcggaa acgaatccaa tcggcgcgat caccacgtca gtcacaccct
     4081 gctgtttgat ctcgcgcagg tgatcgagga tgtccggttc cagccacggc tgcgacgctg
     4141 ggccgctgcg actctggaaa accagtgggt ccggcgtgcg ttccagcgcc tcggagacca
     4201 gtcgtccaac ttccagaagc tggcgctgat agtcgcaact ctgcgacatg ccgagcggaa
     4261 tgctgtgtgc tgtatacgcg atgcgggccg aggcgcggcg atcttctggg atgcggttga
     4321 gtgcctcgtg cacatgatca acattggctt cgacaaacag cggatggttg tagaacacgc
     4381 gcagcttatc gacttgcgga gcgccgtctc cggccgcagc ctgtgccgcc gcaatgtttt
     4441 cgcgatactg tctgcaactg gagtacgaac tataagctga cgtcgcgaag gcgatggcgc
     4501 gtttcacccc gtcggccttc atctgccgca gagtgtcggc cagcatggga tgccagttgc
     4561 ggttgcccca atagatcggc aggtcagggc cgtgtccctt gagttcggcg ctgagagccg
     4621 caattaacgc cctgttctga tcgttgatgg ggctctttcc gccaaagagg tagtagtgtt
     4681 cggcgacctg caacattcgt tcgcgaggaa cgtttcttcc tcgcagcacg ttctccagaa
     4741 atgggagcac gtccgcattg cactccggtc cgccgaacga gaccagcagg atagcgtagt
     4801 atttcatccg cgccggatca acccttcttc tgcagttgcg ccagtgcggc gagcacttgt
     4861 tcgctgtgct ggttgatgtc ggcatccacg tcggtataga cctgcacgat ctttccctcg
     4921 ggattgatga tgaaggtata ccgtttggaa tacttgacca gacccaggtt ggtcagcgaa
     4981 ccatactcct cggaaacctt gtgatcggtg tcagacagca gcttgaaggt gagtccttct
     5041 ttcgtgcaga actcctggtg cgatttggtg tcgtccacgc tgacgcccag cacgacggcg
     5101 ttcgcggcct ggaacttggg ctggtcgcgc tgaaaattgt gggcctcgat ggtgcatccc
     5161 ttggtcatgt ccctggggta gaagtacagc accacccact tgccgcggaa ctgcgaaagc
     5221 gtaatgggat tgccgtcctg cgagttgagg gtgaagttcg gtgccgcgct gccaaccgac
     5281 ggaacggacc cgtcgaatgc ggtcgataca atcatcaaag tgagcaggcc gactgcacca
     5341 accaatccga tctgtttcat catgtgcgta tttctcctga agggacaaat gaagtgcgag
     5401 tgacaatccc cgtcgaactc acttggatgc actatctggg cggacgattc gagttcaggc
     5461 aacagtcgga gttctggtat gaggcgctcg accggcgcca agaccgctcg catcgtcgcc
     5521 cggaacaagc gaggttcttc gccgtgggcc tccgtcacac ggtggtgcgt gtttggtcac
     5581 agaactgttg gtcgcgaaag cttaaaatcc gtttacccgc gagttacgct gatcgggcgc
     5641 agccgtcgat aggcccagtt tcggaggtac atcttgaaaa tcgctcgctt actgccggcg
     5701 ttctcggttc tttttctact gatcgcgttt tgcagcgcgt acgcgcaagg acctccgcag
     5761 ggctccatcc tgttgaagag cccctcgatg ggggcagtaa agtttccgca ctccgctcat
     5821 ctcaaggtgg cgggtcgatg cgaggtctgc catcacgcgt cgaaacctgc taagtctctg
     5881 aaatcgccgc aggaagcctg caccgactgc cacaccaagc cggctacccc gccggtgacg
     5941 atcggccttc aggcggcatt ccataacccc acggccaccg ccgggctctg cgtcacctgt
     6001 catcagacag aaaacagcca cggcaaagcc gctcccgtaa aatgcgccga ctgccacaag
     6061 aaagagaatt cctagaactg gtttgcaagg gtacggctcc gcggtctaac acaccgtctc
     6121 agtgcggctc gagggctatt ttcgcgttct tcaccatctc ggtgtagaac tcatcggccc
     6181 cgttgaacat gatctggatc ccgatgcaga ccaggatgaa cgcagacaac cgcaccacga
     6241 tgctggttcc gttcgcaccg agaaaacgtt cgagtgcgtc agagaatcga tagcagagat
     6301 agatcgtgat tccgatcagc acgatcccaa tggttgaaat cagtgcactc tccaggagtt
     6361 gcatgtggct ctcttcgtgg gcattgctac ccagcgtgag cgctacagcg atcgaacccg
     6421 ggcctaccgt aagcggcatg gtcaaaggat aaaacgcctg cgacaggatg gtctcgctgc
     6481 tgggctgttc cggcacgtga cgttccgaca agttgctctg ctggttaagg attcgccagc
     6541 ccgcgaccac cacgaccaag cctccgccca actgcaccgc gccgatgctc acaccgaaga
     6601 aatccaggac gtacgaccca atgaagagcg aggccatgag cagaacaata ccgttcaccg
     6661 cgacccgcat ggcgacggtg cgccggacgc tcaaatcgta gctttccagc attccggcaa
     6721 atatcggcgc cattcccaac ggattgacga tgggtagaag ggcagcgtac accaacagga
     6781 tgttacggac gatcgcgtac atgggagaaa cagcatatcc gaaattacgc atcgatgcgt
     6841 aacggaatgc ggcccgttga ccaactctgg atttgcggtt tcaatgggag aaccagtacc
     6901 ggagacagcc gcaatcgatg acagacaaga agggaatccc ggcgcgcacc tatggagcgt
     6961 tgcagattca actgcgcgaa tctttattta ctcgcacgct cgtgctgccg attcaggaat
     7021 tcattcacat ccagggcatg agcagcattt tgctgctggg agcggctatc gccgcgctga
     7081 tctgggcaaa ctctccctgg cacgactctt accatcatgt ctgggaactg caactcacgc
     7141 tttcaagcat tcagcttccc gtgcacgcct ggatcaacga tgctctgatg gcgatcttct
     7201 tcttcctggt gggaatggaa atcaagcacg agatcgtgca cggtgaactg tccgacatcc
     7261 gcaaggctgc tttgcccatc gtaggtgcgg tgggcggaat ggtcctgccg gcgctgttgt
     7321 tcgtagccgt caatttccgc gggcctggac aacacgggtg gggagtcccc atggccactg
     7381 acattgcctt ttcgcttggc gtccttggca tggtgaaagg catcccatcc gaactaaaag
     7441 tcttcctgct gaccctggcc atcgccgacg acatcggcgc tatcatcgtc atcgcgtttt
     7501 tctataccga tacgctgcat gtgcaggcgc tgttcgttgg actcttcttc cttctggtga
     7561 tgttcggttt gcgccgcatc ggcatttcgc gggctattgt atttgtcgtc ctcggtgtcg
     7621 cgttctgggt cgcgattctt cagtccggcg tccacgcaac catcgcgggt gtgatcttgg
     7681 gattcatggt tcccacgacg ccccggcttt cactcaagga gttccaggaa atcggcgttg
     7741 acatgatcca ccgttttcgc gcggctcggg cggaaggcga ccttgcaact gcgaatcgcc
     7801 tgctgggaag cttcgaacaa ctggtgaacg ccaccgaggc cgactctgag cgcttgaccc
     7861 gcaagttgaa cgattgggtt tcgtttctcg tgctgccgtt gtttgcattg tcgaacgcgg
     7921 gcgtgacctt tgccggaggc gcggtcaagg aactcactgc cagtcgaatt gtgtggggcg
     7981 ttttgctcgg cctggtgatt gggaagccgc ttggaattgt cggattctgc aaattggcgg
     8041 tgaagttagg cttggcgcgg cttcccaacg gcgtgacgtg gctgcagatg gccgcagtcg
     8101 gcacgctggc tggaatcggg tttacggttt ccatctttat cagctcgctg gcattcgacg
     8161 aacccagcca tttgatggaa gccaaaactg ccgtgctcgc ggcctcgcta atagctggat
     8221 tggcgggatt tttcgccttg agacatgagg caaaactgga aggcgctgcc gaagttcgtg
     8281 tcgaggacta agttgaatcg cgctttccgc aagcgagata caatgaccct ggtattcggc
     8341 atgctcaaag tgcgtctgcg tgacgcgatt tttcactttt ggagcaggta atccgctata
     8401 aggctattga gagcggttat gcactttgtt aacgtgctca ttcacccgca gtagctggat
     8461 tgaatcccgt ccggcattcc aaagagaaag agaaaaagga gacattcatg cgttctaggt
     8521 ttttgacagt catgctgggc gcaattctgg ccatgggcct ggcggcctgc agcaagaaag
     8581 agcagcccgc tcagactcca ccggccacga ccgatcaatc tgcgcagccg actccagcag
     8641 caaaccctca gacccaggcc gcacccggac aatccgccgc ggcccccgcg gcttcaccga
     8701 caggggctcc tggcgaggcc ccagcggccg cgccggcagc agctccggtt gcggaacagg
     8761 cgcctccacc cccgccgcct gagcctatcg tcattccggc tgggaaaact atcacggttc
     8821 ggatgggcga agggctctcg tcgaagacgg cccaggacgg tcaaactttc actggtacgc
     8881 tggccaacgg aatcgctgtt aacggcaaag tcgtcattcc ggcgggctca ggagttaccg
     8941 gaaccgtgtc cgatgccaaa tccgcaggca ggtttaaggg cgaagcgatc attgccatcc
     9001 ggctcacctc catcaacgtc aaaggtgcgc cgcataatgt tggcaccgat gaatactgcg
     9061 tgacccaaaa aggcaagggc aagcgcacgg cggtcatggt tggcggcggt gcgggtggtg
     9121 gtgcccttat cggcggcatc gccggcggtg gcaagggtgc cctgatcggc ggtctggtcg
     9181 gcgcaggcgc tggaacggcg ggcgcggctt tcaccggcaa caaggaactc accatccccg
     9241 cggagtcggc cgtcagcttc aagacaacca cggcgatcac cattcaaccg cagtcgtaat
     9301 ttgtcggggc ccgattaacg gctcgagtcg tggcactgag gggaggcttt cgagcatccc
     9361 tgtcttcttt gtacgcgcag gtgtgagcct ctacactgaa acggagagag ccgtgagcaa
     9421 gagcctgccg ctgacgattc gccacgccat gtacaacctg cgtggcggct ttttgattcg
     9481 cccgctgacc atcgcactgg ctttgggttg cgcgggcgga ttcctgtcgt ggttggaaga
     9541 ggagtttccc gccgtcagtt cctgggtgcc ctcaactctg ttcccctcgc acgccgaccc
     9601 ccaagttgcc cagatcattc tggcggacat tgccggctcc atcatgacgg tggtgtccat
     9661 cgtgttcgcc atcctgctca tgacgcttac cctggcgtcg atgcagttct ccccgcgcat
     9721 catcgtcagt ttttcccgag accgggtgac gcaatggaca ctcgggattt tcctcggcac
     9781 gttttcgtac tgcatggcag cgcttccggc ggcgcgctcg ctccctcacc ccttcgcccc
     9841 cgtggccacc gttcttggag cgatggtgtt ggcgctcgtc tgcgtcgggt tgctgctctt
     9901 tttcattcac cacatctcgc aggccatcag tgtcaatcac atcgttgacc ggatcgcgga
     9961 agagaccgag gccatgatcg acgagatgat gccccggccg caccacccgc accgcatgaa
    10021 cgatacggaa ccgctgcggg ccaattcgac cgagattgcc gttccgagcc atgagtccgg
    10081 ctacatccgc ttcatcgata cgcggcgtct ggtggccttt gccaagcacc accacgtcag
    10141 cgtccgggtg cttcgacggg ttggacaatt tgtaccggcg ggaattccgc tgatgatggt
    10201 gtcgaagggt aaccgcctgc ccgccggcgc gagcgacgag ctgcttgcag cgttcgacat
    10261 cggcccgacc agaactctcc agcaggacgt ggagtttggt gttttgcaga ttgtcgatgt
    10321 tgcgttgaag gcgatctcgc ctgccgtgaa cgaccccacg accgccatca cctgcgtaga
    10381 tcaattgagc cgcattctga tccgcttcgc gtcccgtgaa actccagacg aactgctcta
    10441 cgatccgccg ggcgtggttc gcgccagtat tcgatggatg cacttcgagc gcctgcttga
    10501 ggcggccttc gaacagatcc gtatgtactc gaaagcggac gtggccgtca gtttgcgatt
    10561 gctgcgcgcc ctcagtgaca tcgctacctc gacgctcgat ccggagtacc gccgtgtact
    10621 cattgaacag ggaatacgga tcgtgacagg ctgcgaagag aagctcggcg aagaagagct
    10681 gagcccgttg cgcgctcgcc aggccacgtt ggagaatctc ctctcgatcg agcaggcgta
    10741 aatgggaaca gcgttgttga cacccacgac cctgcgcagt taggtctgtt tggcactctt
    10801 tatctttgcg cgcttttccg cagcccaatt cgatttgtta acctgccggc tgcggctcag
    10861 ggctagagcg tcggacggta cgtcctcggt gatgcacgat cccgccccga tatacgcgcc
    10921 cttgccaatt tttacgggag ccaccagcgt cgagtcgctc ccgacgaaca ctccgtcttc
    10981 gataaccgtc tcgtgcttgt tcacgccatc gtagttgcag gtgattgttc cggcgccaac
    11041 gttcacacct tcgccgatca cagcgtcacc cagataggta agatggttcg ccttcgagcg
    11101 cttgcccagt tttgtcttct tggtctcgac gaaattcccc acatgcgctc cctcgccgac
    11161 atcgcttccg gaacgcagat gtgagtacgg gccaatttgc gcaccgtcgc gcaccacgga
    11221 actctccatg atgcagcccg gacgtatgag caccttgtcg ccaatctcgc aattggtaat
    11281 taccgagtaa gagcgaatgc ggcaatccga accaatcttc gtttgtccaa tgatctgcac
    11341 gaacggctca atcacggtat ccgtgccaat ctcgacgtcg gcatcgatgt cgcaagtctc
    11401 ggggcggaag atggtagtcc ctgactccat cagttggcgc gctttcttcg cgcgcaactt
    11461 ggcgtcgagt tccgccaact ccatgcgcgt gttaacgccc agcacttcat ttgaatcttt
    11521 agcgcgaagc gcgaccactt catttcccga cttgccgagt actcccgcca tgtcggtgag
    11581 gtaaaactct cccgcaggat tgtcggtcgt gagctcatcg atgtgcgtgt atagcggcgc
    11641 aatcgcgaaa gcatagatac cggagttgat ttctcgctgc tcaccttcgt tattgcgcag
    11701 ggccttctgt tcaacgatgc gatccacttc gtcggtgggc tcaccgccct tttgtttacg
    11761 gaagactcga ccatatcctg tggggtcggc tggctcagcg gtcaggaccg tcatcgccgc
    11821 ctggttggca atgtgaaaat cacgtacccg ctgaatcgtc tcgggatgga tgagcggcac
    11881 gtctcccgac agcaccagca cgtgtccgaa cccttggagc gcttctcgtg cggccatcag
    11941 ggcgtgtcct gttccgcgtt gctcgcgctg gagaacgaac ttaattccgg tgcactgcag
    12001 tgcctcgcgc accagttccg cctcgtgacc aatgatggcg aagatgttct ccggcggaac
    12061 gatcttgctg gctgccgcga ccacgtgtcc taacaaggtc ttcccggcaa tctggtgcag
    12121 caccttggga tgccgcgact tcagtcgcgt gccttttccc gcggcgagaa ttgcaatggc
    12181 gaagtcaaac ttgctttggc ctgcggagtg ggcggagccg gactgcggat tcatcatgac
    12241 cttacagggt atcaagagaa gcttaggatt tgtgttttgt ggcggcgacc ggggcttcgc
    12301 gcgtgtcaag cagcaggcgc aaaagatcat gcgccacgtg gtgggtgtcg tgccgggcga
    12361 tcagaccttc attgggatcg aagtcttcgg tgagatagtc gccaagaatc ggctttacac
    12421 ccaaagcctc gatctgctcg gcatccacca cgatttgcga ctgctgcttc tcggcgtact
    12481 tcgccgccag ggtaggagac gcagccgtgt ggttgaccag ggcatagtcg aagaactcat
    12541 gccgcgcgtg attgtaaatc gcttggatat gctgcgatgc cgtgaggttc aggctttcat
    12601 tggcctgtgt catcaggttg caaacgtaga ccttggtggc tccagagttc ttgatcgccg
    12661 cggaaaggcc gcgcaccagt agattaggca ccaggctggt gaacagcgat cccgggccga
    12721 tggtgatcaa gtccgcgctt tcgatggctt ctagggctgc ccgagcgggt ttcgcattcg
    12781 ccggggccat ccgcaattct gtgatgcgtc gccggcttgc ggttatgttg gtttccccgc
    12841 gcaccaccga gccgtcatcc atgatcgcgt agagttgagc gttactcgtg gtcgccggat
    12901 agatatggcc acgggttgcg aggatggagg cggtatatct caccgcttca ctgaaatctc
    12961 ccgtgatgtc ggtcattgcg gagagaaata gattgccgaa gctatggcct tccagtcctt
    13021 cccccgcctt gaagcggtat tggaacagcc tcgacagcag ggcttctttt tccgccagcg
    13081 caacaatgca gttgcgaatg tcacccggag gcggaacgtt cagctccttg cgtagtctcc
    13141 cgctggaacc gccgtcgtcg cttactgtca caatggccga cagatcggag atgaacggaa
    13201 gtaaccggtc aaacgcctct tcgcccggca ggattacgtg ctgcttcaat ccgtggagca
    13261 gcgtcgagag tcctgtcccc ccgcccatgg caacaacctt cagggggcgc acttcggagg
    13321 aatggttgga actctgcatg gccagatcag aaattgctag aaatgccact ttggtgaatc
    13381 ggatggcact tctgcgctgg tatcaggata tagcagttct gtgaaacgcg ggtcgtcaga
    13441 aagactttta aaatcggaat cgtttcgcgc ctgaaaacgg ttggccggat tcaaccggat
    13501 cgcctcattc agccccgcga tcgcatctgg aaaatgtccc atcaggcagt tcagcgcggc
    13561 ggccccgtac cacacgaagt ccaacttggg atgctgctgg gagagatcac tgaagacctc
    13621 tcgcgccctg atgtagtcac ccatgttcat gagtgacacc gcataatcgt attgctcttc
    13681 gggggtcttg aacttggccg ggtcgcgatt cagttgctgg ttgcaggtgt tgagatggac
    13741 gagtgcgcgg tccgccaatt cgggactcga tccaccaacc agtttctcaa aacaagcctt
    13801 cgctttatcg tacttgtgag actgcagcgc cttaagtcca gcctcatagt tttgcacagc
    13861 ctgtgaaaat cgcggatcgt ccgccacctt caagggcttg gcgcttcgag cgcggatttc
    13921 tgatcccgtc gaggatgcga gtaagtctgg ttgtttcttt gttttacctt tggtttcctg
    13981 aacgttttct gcctcttttt tcttcatcca gttacatcct ttgaatcgta aacggcaggc
    14041 aacccgtcag ggttgtggaa aacccaaata ttacaggctg tgtgtgctga gttatacccg
    14101 caacgtgcgg tggcgtcaac tgtacctagt gactaggccc gctgcggctc ctagttcatg
    14161 ttcgctgcag cctcaagatc gatcgatttc cattcgccct tgatgattcc ggcacgaatt
    14221 tgggcaggcg tcttcttcag cttggtctcc tgaaatgcgt agtacacctc tttcatgcag
    14281 atgccgcaat gcgcaccgtg attggattca aagcaggtgt ggaggctctt gtgtccgatg
    14341 cgctcgcaat agcaatagca gggaagctga tggatcacgc ccggaatctt cgccgccaga
    14401 tcgtaggcgt gtacctggta gggataccta aagacctctc ccactcgttg gtccgccggg
    14461 aggatcggta gcaactctcc cgccagcgga ggacgcaggt tgtaggcggg aacgctgtcc
    14521 ccctgagcga atgccatcac cgctaacaca acaatcaatg cgagtatccc agacttcctc
    14581 attgccacaa cctcggcggt tattctagct gagaatcccc caggccccca aacgacgtgg
    14641 aggggttgaa gtccgcgcca gcgcaactcg ctgccttccg acggtgttct ctataatgga
    14701 ccaagcacct gaaccccatc catgagcttc atgccaactg attccaacat gccctcgtcc
    14761 atcgggactc atccgcaacc actcgggcta gacgagcgaa ccgcacgcgg ccgcattccc
    14821 ccggtgttcg caagatgggt cagggacatc ctgatctcgc tggccgtgtc agcattcttc
    14881 atcatctttc tctaccaacc cgtcaaggtc gagggaacca gcatgatgcc gacgctcgaa
    14941 gaccaggaac gcgtcttcat caacaaattt gtgtatcggc tggaagcaat cgagcggggc
    15001 gatgtggtgg tcttccgcta tccgcgtgat acctcgaaga gctacatcaa gcgcgtgatt
    15061 gccgtcgccg gcgatcgcgt aaagatcgac gacggcctgg tctacgtcaa cgaccgccag
    15121 atcgccgagg gctacgttcc gcaggtgtac gaagatgtgc gctcgtatcc cgagactgtg
    15181 gttcctcctc attcgtactt cgtgatgggc gaccatcgca atctgtcgaa cgacagccgt
    15241 gacttcggtt cggtcgacga aagttacatc tacggcaaag ccgtttttgg ctattggccc
    15301 ttcgccaagc tgggcaaact gcgttaggta gccttgatgc agtccgcggg gtagtgccgg
    15361 ccttcaggcc ggcatcccag tgagtgtaga aattcgggtt tcggcgccga tgtacccgaa
    15421 ctggagttgt tttagaatcg ccttggccaa aactgcactt aacggaaggt ctatgagagc
    15481 aaccctgcaa ctcctgttgt ccatcttcgt gctctcagcc atgtccttcg ccgccgaccg
    15541 cgatcccatc gttctgatgc aggccgagcg cgacttcgcc aaggccactg ccgatcgtgg
    15601 cgtcgatggc tggatggagt tcatggcccc caatgcagtc gagttatccg cggagccgct
    15661 ggttggtctt gatcagatcc gcgccggcat gaccaagcag ttcaaattgc cgggcttcaa
    15721 gatcacgtgg gagcccaccc atgcggagtt cctcggacag ggcgacatag gctacgcggt
    15781 cggacgctac gaggttcgct tcaccagcga agatggcaag ccccagttcg agaagggcag
    15841 ctatctcacc acctggcaga agcaaaaaga cggctcgtgg aaggtcgtgt cagacatcgg
    15901 cagccccgat cctccggcac attgagaaaa aatccctcct cttatgctcc ggcgcgatcc
    15961 ctgataaaat cgataaaatc cactcctcgg agatgcaatg caggcaatcc ttgcgctgga
    16021 agacggacgc atcttccacg gcaaaggctt cggcgccaaa ggcgaatgtt acggcgaagt
    16081 tgtttttaat acttccatca ccggctatca ggaaattttc accgatcctt cttatgccgg
    16141 gcagatcgta gttctcacca atccccagat cggcaattac ggcaccaact ctgaagacaa
    16201 tgaagcgacg cgtccttaca tcgagggcct gatcgtccgc gaattctccc gcgtcagttc
    16261 caactggcgg tcgcaggaag tcgccgaaga gtatttggag cgctacaaga ttccagtgct
    16321 cgccgaaatc gacactcgcg ccctcgtgcg gcacttgcgg acttatggcg tcatgcgcgg
    16381 cgtcatttcc acaattgaaa ccgacaccga caaactcgtc gccaaggccc gctccattcc
    16441 caagatggac ggcaccgacc tcgcgaaaga agtcaccact ccccgcgtgt atcagtggga
    16501 ggaaggcgtg cgttccttcg agccgacgga agtggtcgga gtcaaggacg acgcgccgaa
    16561 gtacaaagtc gtggcctacg actacggcat caaacacaac atcctgcgtc gcctggttgg
    16621 tgaatcatgc agcgtgaccg tggttcccgc gaccaccagc gctgaagacg tgctcgccat
    16681 gaagccggat ggagtgtttc tgtccaacgg tcccggcgat cccgaacccg tcacctacgc
    16741 gcaggcaagc attcgccgca tgatgggcaa agtccctatc tttggcatct gcctcggcca
    16801 tcagctgatt ggcctcgctc tcggcggcaa aacctataag ctacgcttcg gacatcacgg
    16861 cggcaatcac ccggtcaaac agatggcaac cggcaagatc gagatcaccg cgcacaatca
    16921 caacttcgcc gtcgaccccg actcgctgaa acagagcgaa gttgacctga cgcacatcga
    16981 cctgaacgac aacacgctgg aaggcctgcg ccatcgttca ctgccgctgt tcagcgtgca
    17041 ataccatccg gaagcagctc ccggccctca cgactcgcaa tacctattca aagactttgt
    17101 gaagatgatg gaggagtgga aggggaagtg aggacgagat tttttcttaa agtgaggtgg
    17161 aagtgccacg aaaaaagtct tctgtaagcg gaatcttttt aagccactct tcccgcgaca
    17221 aggagtttgt tagtaggctg gcgagcgatt tggtgaaccg cgggtttccg gtttggtttg
    17281 acagctggga aatggagatg ggagattcac tttatagcaa aatctatgaa ggaattgatt
    17341 ctagcagcgt actcattctg gttatgtcaa agaatgcggt caagtcggat tgggtcaaga
    17401 aagaattgca tggggcactt ctgaaggaag aggaactgaa gaacagattc ttgcttccga
    17461 taaagccaga ggaatgcaag gtaccgttct cgatccgcga tcgtatttat gcggacttca
    17521 gtaccgatta tctgatgcaa ctggagaggc ttgaaaaggt tcttcgggct aggggcgcag
    17581 ataagattga tgtagcgttt cagaaagagc tagtcccttt aaggatctct aggggcttat
    17641 acctcgacga aatctcgttc caatctcgga tttcagcatt ggtgccgcga ataaaagcgg
    17701 ggaaccaaat cgaggtttca caactcgtgc tttacccaga acctgaatac ggtgatgctc
    17761 gacgattggc tacgcggagg attaacgagc tggataaaag ccaaaaccac acccccgagg
    17821 ctgaagtgga ccttcgctca acttacgaat cgattaggcg acttgaagat gcgcttctcg
    17881 tgggtgtcgc gggaatcatg aacggattgg tgaagatgaa cgagtgggca tttgtgggac
    17941 tcgcttgtca ttggtatgtg cgaatcattc ggtcgatgtt gttgttcaga atttggcggc
    18001 tgcagtcaga gttatccggc gccactgaat tcggcaagga cgcattggca gacccagtca
    18061 ataacgacac ggctgctcgg ttctttggga ttgacgatgt catttgctgc gacgtctgga
    18121 agccaccttc aaatgagtac tactcgtttt tcgttgatgc agactctgag gttggacgat
    18181 ggttccagga gcatcctcaa tgtcccgacc tgctaagagc attttggtca ccaaccctta
    18241 tgtacaagta cgcgataccc caaatggtct acaagtccta tacaaatcca gcaccgttcg
    18301 gctgggattt cgcggattgc aaaatcggac gtcattgaga accgatgcct aaacgcaacg
    18361 acatagcgaa gatcctcatc ctcggctccg ggcccatcgt catcggccaa tccgccgagt
    18421 tcgactactc cggcgcgcag gcctgcaagg cgctgaaatt cgaaggctat gaggtagtgc
    18481 tggtgaactc caacccggcc accatcatga ccgatcccga gatggctgac cgcacctaca
    18541 tcgagccgct caatcgcacc tatctcgaag agatcattcg cgtcgaagcc gaaatgctcg
    18601 ccgagagcgg ccgccccggg atcttcgccg tgctgcccac ggtcggcgga cagaccgccc
    18661 tcaacttggc cgtcgaactc gccgacactg gcgtcctcga gaagtacggc gcacaactca
    18721 tcggtgctca actcgacgcc atcaaaaagg gtgaagaccg cttgctcttc aaagacgcga
    18781 tgaccaagat cggcctcgaa gtgcccaagt cctccctggt taacaacctg aaggacggga
    18841 tggacttcac cagcaaaatt ggcttcccgg ttatccttcg cccttcgttc actctcggcg
    18901 gcaccggcgg gggcatcgcc tacaaccgcg aagagctgct cgaactcctg gctcgtggcc
    18961 tcgacctctc gcctgttcac gaagtgttgc tggaagagtc tgtcctcggt tggaaggagt
    19021 acgaactcga gttgatgcgc gacttggccg acaacgtcat cgtcatctgc tcgatcgaga
    19081 atttcgatcc catgggcgta cacaccggcg actccatcac agtcgcgccc gcgcagaccc
    19141 tcaccgatcg cgagtaccag gccatgcgcg acgctgctat cgccgtgatc cgcgaaatcg
    19201 gcgtcgagac tggcggctcc aacatccagt tcgccgtcga tccccacaac ggtcgcatgg
    19261 tcgtcatcga gatgaacccg cgcgtgtcgc gttcctccgc gctggcctcg aaggcgacag
    19321 gattccccat cgcgaagatc gccgccaaac tcgctgtggg ctatacgctc gacgagatca
    19381 ccaacgacat cacgcgcaaa actccagcct gcttcgaacc cacactcgac tacgtcgtgg
    19441 tgaagattcc caagtggcag ttcgagaaat tccacggcgc tgatgaatcc ctcggcccgc
    19501 aaatgaagtc cgtcggcgaa gtcatggcca ttggccgcac cttcaaacaa gcgttgctta
    19561 aagccgtacg ctcgctggaa acgggcaaac gcatcggctc tgagaaaatc gagccgcgca
    19621 ttctcaccaa acgccttgtc actcctcacc ctgagcgcct gcaatacatc cgttacgcgc
    19681 tctcgcaagg aatgaccgtg cgcgagatcg cgcagatgac caacatcgat ccttggtttc
    19741 tgcatcagct caaggaaatc tccgacgaac aagccaagct gagcagcctt ccgctcgaca
    19801 ccatcggcac agccgcgttg cgccacctca agcgggtcgg cttttctgac gcccgcttgg
    19861 gcaactcgct tcagaccaac gaaggcaaag tttccgccga gaagattcgt cagcttcgca
    19921 agcaacatgg tgttcgcccg gtgtacaagc gcgtcgacac ttgcgccgcc gagttcgaga
    19981 gtttcacgcc atacatgtac tcgacctacg aggacgaaga cgaggccacg cccaccgatc
    20041 gcaagaaggt cattatcctg ggtagcggtc ccaatcgcat cgggcaggga atcgagttcg
    20101 actactgctg ctgccatgcg gccttcgctc tgcgcgacga cggctacgag acgatcatgg
    20161 tcaactgcaa tcctgagacc gtctcgactg actacgacac cagcgatcgc ctctacttcg
    20221 agccgctcac ctacgaagac gtcatggcgg tctatgagca cgaagccacg ggtggcgccc
    20281 cagtcggcgt catcgtacag ttcggcggtc aaacgccgct caacctcgcg cttcctttga
    20341 aaggtgcagg cgtgccgatt attggaacct cgcccgagag tatcgatctc gccgaagacc
    20401 gcaagcgctt cggcaagttg ctcgaagagc tagatattcc ccaacctccc ggagctactg
    20461 ccgccagtgt ggaagaggcg ctcgccaacg cgaaccgtgt cggctatccc gtactggtgc
    20521 ggccatctta tgtactcggc ggacgcgcca tggtgatcgc ctacgacgac gagtcggtcc
    20581 atcgctacat gaaagaagcg gtcgagtatt cgcaggaacg gcctgtcctg atcgaccact
    20641 tcctcgagtc ggccaccgag gttgatgttg atgccctatc cgacggagaa gatgtcgtga
    20701 ttggcggcat catgcagcac atcgaagagg ccggcattca ctccggcgat tcttcctgcg
    20761 ttctgccgtc agtggacctc tcgcccgatg tgctggccac gattcgcgac tacaccttcc
    20821 gcctcgcgcg cgccttgcaa gtcatcggcc tgatgaacat ccagtacgcg attcagcgcg
    20881 acaaggtgtt cgtcatcgaa gtcaacccgc gtgcctcgcg caccgtgccc tacgtctcga
    20941 aggccacagg agtaccgctc gccaaaatcg ccgcccgcct catgaccgga cgcaagctgc
    21001 gcgaattcct tccagaaaac gtcgaacgcg gcactgatct agacaccggc agcttctatt
    21061 atgtgaagtc gccggttttt ccctggggca agttccccgg cgttgacacc gtgctcggcc
    21121 ccgagatgaa atccactggc gaagtcatgg gagtggccga cagcttcggc gaagcattcg
    21181 ccaaggcgca agccgctgcc ggacaagttc tgccgacctc gggtcgtgtt tttatcagcg
    21241 ccaacgatcg cgacaagccc ttcgtcgcgg aagtcgcgca gcgcttcgtc gaactaggct
    21301 tcagcctggt tgcgacccac ggcaccgcaa agattctcga agacgcgggc ctgcaagtcg
    21361 agcgcgtgta caaggtgaaa gaaggacgcc ccaacgtcgt tgacctcatc aagggcgaac
    21421 ggcttcatct aatcgtcaac acgcctcacg gcatcgatcc ctggttcgac gagaaggcga
    21481 ttcgccgggc ggccgtgacc gcccgcatcc caacgatcac gactctgtca gcggcccggg
    21541 ctgcgtgcga aggaatcgca gcgctgcaac gcggagacat caaggtgtac gcgttacagc
    21601 aactgcacgc cgagcgcgca gccgctgcaa agtagaagga aactgtcatg ttcttacacg
    21661 gaatctcccc cccgatcacc accccgttct acacggacgg caatatctat ttccgcaagc
    21721 tcgagcagaa cgtcgagcac tattcgcgca cgcccaccgc gggcatcgtg gtcctcggct
    21781 ccaccggaga ggccatcctg ctcagcgaca tcgagcgccg cgaagtcctg aagaccgccc
    21841 gcgatgccgc tgccaacgac aaggtgctca tcgccggaac cggcgtcgag tccgccatcg
    21901 agaccctgcg cctcaccgag ttcgcctacg acgtcggata cgatgtcgcc atggtgcgca
    21961 ccccgcacta ctacaaagga cagatggcca acccgcaaaa catcctggcc ttctaccgtt
    22021 tcgtgtgcga ccgctcgccc ctgcctgtca tcatctacaa cttcccccag gctaccggat
    22081 acgacatccc cgccgagctg gtcatcgagc tcgccgagca ccccaacctc atcggcatca
    22141 aggagtcatc gggctccatc gagaaagtgc ataagatggt cgagggcacg cgtcacattc
    22201 aatgtaaggc caccgtcacc gaaacgttcg cggccgtcac cggacgcatg ctgcaagcgg
    22261 ccgccacttc ccccagcact gagggtggcg cgctggtttc catcggagcc cccgcctcca
    22321 gcgccgcttc tgacaagact cctgcgccca agccctcgtc gagcgcggtt aaggttgtgg
    22381 gc
//
LOCUS       DASRKK010000407         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1399729, whole genome shotgun sequence.
ACCESSION   DASRKK010000407 DASRKK010000000
VERSION     DASRKK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399729"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1077)
                     /locus_tag="VFC15_09315"
     CDS             complement(25..1077)
                     /locus_tag="VFC15_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM10395.1"
                     /translation="MPTSSGMLRVGVVGAGAFGRNHARVYRDLQADPSQNVEFAGIVD
                     TDFPRAQTVASEFGVHAFRSLDELIAAGMQACSLAVPTAAHLEVARQLMQNGIDVLIE
                     KPLATTVAEADELIAIAKQHGRIAQAGHLERLNPAVRATFPLLTQPMFFEVHRLSVFS
                     PRSLDVDVVLDLMIHDLDIVLAFVQSPVKEVRAVGLPIRSQKVDIANVRMEFESGCVA
                     NFTASRVSTERVRKLRFFQPRQYVSLDYSRQDVLVLTVDDPSLDPAGLDPFNPASVAA
                     DALPGITPFRPAIGQQEPLRAELDSFLRAVRDRSVPEVTLEDGRRVLALAMQVVDAIA
                     EHSRRVHLAELTRTPS"
     gene            complement(1264..2151)
                     /gene="lpxI"
                     /locus_tag="VFC15_09320"
     CDS             complement(1264..2151)
                     /gene="lpxI"
                     /locus_tag="VFC15_09320"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HZM10396.1"
                     /translation="MTDPSQKIGLIAGNGKFPLLVLDAARSQGMQVVVAAIKEEASPE
                     IEQRGAAAVYWLSLGELSRLIDTFKQEGVTRAIMAGQVKHKQIFSSIKPDWKLAKLLL
                     SLTTRNTDSLIGAIGKVLGEEGITLMNSTALLEPMLASEGVLTRRGPTDSEQTNITYG
                     RAVAHHLSRFDIGQTVVIAEAACVAVEAMEGTDATILRAGEIMQSPSLGKEPSTLSRA
                     LTVVKVAKPNQDMRFDVPVVGLKTIATMKQAGATCLALDAERCLLLDREALLSAANDA
                     EICVVAETPVSHIPHHYVP"
BASE COUNT          482 a          699 c          712 g          430 t
ORIGIN      
        1 catcggggtt tcgagaagcg taggctagct cggcgtacga gtcaactccg ctaaatgcac
       61 gcggcggctg tgttccgcaa tggcgtcgac cacctgcatc gccagggcga ggactcggcg
      121 gccatcttcc agcgttacct cgggcacgga gcgatcccgc acggcacgca ggaagctgtc
      181 caactccgca cgcaacggtt cctgctgccc aatcgccggc ctgaacggtg taatgcccgg
      241 aagggcgtcg gcagccacgc ttgccggatt aaatgggtcc aaaccggccg ggtcgagcga
      301 tgggtcatcc accgtcagca ccaatacatc ctgcctcgag taatcgagcg agacgtattg
      361 gcgcggctgg aagaagcgca gcttgcggac ccgctcggtg ctgacgcggc tggcggtgaa
      421 gttcgcgacg caaccggact caaattccat gcgcacattg gcgatgtcaa ctttctgcga
      481 gcggatcggc aaaccaaccg cgcggacctc cttgaccggc gactgcacga acgcgagaac
      541 gatatcgaga tcgtgaatca tcaggtcgag gactacgtcc acatcgagcg agcgcgggct
      601 gaaaacgcta aggcggtgca cttcaaaaaa catgggctgc gtgagcaagg ggaaggtggc
      661 gcgcaccgcg ggatttaagc gctcgaggtg gccggcttgt gcaatccggc catgctgctt
      721 ggcgatggca atcagctcat ccgcctcggc caccgtagtc gccagcggct tttcaatcag
      781 gacgtcaatg ccgttctgca tcaactgccg cgcaacctcg aggtgggcag cggtgggcac
      841 cgcgagcgag cacgcctgca taccagcggc aatcaattcg tcgagcgaac gaaacgcatg
      901 gacgccgaat tcgctggcca ctgtttgtgc gcgggggaag tcagtatcca cgatcccggc
      961 aaattcgaca ttctgcgacg ggtccgcctg aaggtcacga tagacgcgcg cgtgattgcg
     1021 gccgaaggcc ccggcgccga cgactcccac gcgcagcatc ccgctgctag ttggcattct
     1081 gcgattgtcg cgcaaagcgt ggcaggaggc aaaaaagcaa gttaaccgcg gattgacgca
     1141 gatgaacacg gatcaagaaa atattctggt cgattgcgcg aaccagtgct tcaagattca
     1201 aagaggtatc cgcgagatcc gcgtgaatcc gcggttcaac acccgcaaat ccttgggcag
     1261 aaactagggc acgtagtggt gagggatgtg gctgaccggg gtctcggcga caacgcaaat
     1321 ttccgcgtca ttcgccgccg acagcagggc ttctcgatcc agcaacaggc atcgctcggc
     1381 atcgagcgcc aggcaggttg cgccagcctg cttcatggtc gcaatcgtct tcaagccgac
     1441 gactggtaca tcgaagcgca tgtcctggtt cggcttggcg accttcacca cggtcagcgc
     1501 gcggctcaac gtggatggct ctttgcccag cgagggcgac tgcatgattt ctccggcgcg
     1561 aaggatggtg gcgtccgtgc cctccatggc ttccacggca acgcaggcgg cttcggcgat
     1621 gaccaccgtc tggcctatgt cgaagcgcga gagatggtgc gccaccgccc gtccgtaggt
     1681 gatgttggtt tgctcggagt cggtcggccc ccgccgcgtc aaaactcctt cgctggccag
     1741 catgggttca agcagcgcgg tggaattcat cagcgtgatc ccttcttccc cgagaacttt
     1801 gccgatcgct ccgatcagcg aatcggtgtt tcgggtggtc agcgaaagca gaagcttggc
     1861 cagcttccag tccggcttga tgctgctgaa gatctgctta tgcttgacct gcccggccat
     1921 gatcgcgcgc gtaacgccct cttgtttgaa ggtgtcgatg agccgcgaca gctcgcctaa
     1981 cgacagccag taaacggcgg ccgcaccgcg ctgctcgatt tcgggcgaag cctcttcctt
     2041 gatggccgcg acgacgactt gcatgccctg cgagcgagca gcatccagca ccagcaaggg
     2101 aaacttgcca ttgcctgcga tgagtccgat tttttgagaa ggatcagtca tgtcagagcc
     2161 gatagcctga ggagcgaaaa ctaattagcc cagcgcggaa gagtctgtgt gagaacatcc
     2221 tccgtccctc caccactctc caaccacaaa tgcggcggtt gtcagggaaa ccggctacgt
     2281 cgtgtttgga gccgatagcc cggagggcga aattaattag ccc
//
LOCUS       DASRKK010000408         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1204173, whole genome shotgun sequence.
ACCESSION   DASRKK010000408 DASRKK010000000
VERSION     DASRKK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204173"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFC15_09325"
     CDS             complement(<1..431)
                     /locus_tag="VFC15_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10397.1"
                     /translation="MVERTGALGYFGDARRAASGTELFERVTATGSLVIRKLGETRAG
                     ELAIHRFLSAPSVTCKEMLGTLAGRTVTATAGRRIVVAQDTTEINFAGREANRRGLGP
                     AGDGVSAGFFIHPLVAIDSETEAVLGLLDAHIWTRDDAIEA"
     gene            complement(547..936)
                     /locus_tag="VFC15_09330"
     CDS             complement(547..936)
                     /locus_tag="VFC15_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10398.1"
                     /translation="MQRKSIPRGSGKRVVQGRDLRLADEVRYIQSRAADRDARIVTIG
                     PLVLFSTQTGDAWLLDPADHLALPLTRDGDPQPAQIEDTDTAFAIGWTGHYHIDGPAF
                     IYIDQRNGRMATILGYPTQKLTQKPRN"
     gene            935..2599
                     /locus_tag="VFC15_09335"
     CDS             935..2599
                     /locus_tag="VFC15_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10399.1"
                     /translation="MLHLAEGAMDLFVTLFGPMLAFVYHCFDRIVIHGYLSSLSRPQQ
                     VVYFFRQVVGIPVVSKEALSERTGIYQKWVDGYARNHKIPIEWAEKKVRKEDYVLPHL
                     RRMTKADAYGAYFIFKSMEQGASFRITVPKYATKDPNHRILARQKSRYTHYYFYIRDY
                     FYIRDEVLGPVVMRVGSFFPFQTTYYLNGHSFIEQELKRAQIGFRKTDNAFLGVDDVA
                     ALQAAADRLSPEIIRERLDYWTFVLGPKFSDKERVAMNLSRFYAINQIEYCRNFIFKR
                     NFPIHKLFERSCEIGLWRLTANRIAEIFGTRLNRRMRGKLATVIDQIEHRHHVFRAYF
                     KNAFVKQYEKFSTFLRNELVSNNLKDFGLKKSLDQLDAVRQKFQTITARFAGFQAQWL
                     NVHADFPLLQHLAMPVIIGRVRYPGIKIHDSRAIRLLEVLLHGSTQVGGWTVREIHQA
                     VLTSFELSDHVYGINQLRYDLRKLKGHGLIERDTSRYAYRLTHQKGTETALLFLVFHK
                     RLCGPLANSRFHHRPDPGDRPDTRLEAAYHRADKAIDNIVQLLEAA"
BASE COUNT          546 a          840 c          732 g          557 t
ORIGIN      
        1 tccgcctcga tcgcgtcgtc gcgggtccag atgtgcgcgt cgagaagacc gagaacagcc
       61 tccgtctcac tgtcgatggc gaccagggga tggatgaaga aaccggccga aacgccatcc
      121 ccggccggtc cgaggccacg gcgattggcc tctcggccag cgaaattgat ctcggtggta
      181 tcttgcgcaa cgacaatccg ccgtcccgca gtcgccgtga cggtgcgtcc cgccagggtt
      241 cccagcatct ccttgcaggt caccgatggc gccgacagaa aacggtggat cgcgagttcg
      301 ccggcacgcg tctcaccaag cttgcggatc accagcgatc cggtcgccgt cacccgctca
      361 aacagttccg tgccactcgc cgcccgccgc gcatcgccaa aatacccgag cgcccccgtc
      421 cgttccacca tgatgacccg cccttcaaga caggatcaga gaatcgacga atcaaccgat
      481 tcacaagtgg tgtttttcga gtgaaatgtg tgaatgccgt agggctagcc aaaaatattt
      541 gaaatttcag tttcttggct tctgtgtgag cttctgcgtc ggatatccga ggatggttgc
      601 catccggccg ttccgctgat cgatgtagat gaaggcaggt ccgtcgatgt gataatggcc
      661 tgtccagccg atcgcaaagg cggtgtccgt gtcctcgatt tgggcaggtt gcggatcgcc
      721 atcgcgtgtg agtggcaggg cgaggtgatc ggcggggtcg agcagccagg catcgccggt
      781 ttgggtggag aagaggacga gcgggccgat tgtgacgatg cgggcgtcgc ggtcggcggc
      841 ccggctctgg atgtagcgaa cttcgtcggc caggcggagg tccctgcctt gtacgacacg
      901 cttaccagat cctcgtggga tggatttacg ttgcatgctt cacctcgcgg agggtgcgat
      961 ggatctgttc gtcacgctgt tcggccccat gctggctttt gtctaccact gtttcgatcg
     1021 cattgtcatt catggctatc tgagcagctt gtcgcggccc cagcaggttg tgtacttctt
     1081 tcgccaggtt gtcggtattc cggtggtgag caaggaggcc ttgagcgaac ggaccggcat
     1141 ctatcagaag tgggtggacg gctacgcccg caatcacaag ataccgatcg agtgggccga
     1201 gaagaaagtg cgcaaggagg attatgtcct gccgcatctt cgccgcatga cgaaggcgga
     1261 tgcctatggc gcctatttca tcttcaagag catggagcaa ggagcaagct tccggatcac
     1321 cgtgccgaaa tacgccacga aggacccgaa ccaccgcatt ctcgcccgcc agaagagtcg
     1381 ctatacccac tattacttct acatacgcga ctacttctac atacgcgacg aggtccttgg
     1441 tccggtggtg atgcgggtgg gctcgttctt cccgttccag accacctact acctcaacgg
     1501 ccacagcttc atcgagcagg agctgaaacg cgcgcaaatc ggtttccgca agaccgacaa
     1561 cgcgtttctc ggcgtcgacg atgtcgccgc actgcaggct gccgccgacc ggttgagccc
     1621 cgagatcatt cgcgagcggc tcgactactg gaccttcgtc ctggggccga aattctccga
     1681 caaggaacgc gtggcgatga acctgtcgcg cttctatgcc atcaaccaga tcgagtattg
     1741 tcgcaatttc atcttcaagc gtaatttccc catccacaaa ctcttcgagc gcagctgcga
     1801 gattggccta tggcggctta ccgccaaccg gatcgccgag atcttcggca cacgtctcaa
     1861 tcgcaggatg agaggcaagc ttgctaccgt catcgaccag atcgagcacc gccaccacgt
     1921 cttccgggcc tacttcaaga atgccttcgt gaagcagtac gagaaattct ctacgtttct
     1981 tcgcaatgaa ttggtttcaa acaatctcaa ggacttcggt ctcaagaaaa gcctggatca
     2041 gctcgatgcc gtgcgccaga agttccaaac catcaccgcc cgcttcgccg gcttccaagc
     2101 gcaatggctc aacgtccatg ccgattttcc tctcctccag cacctcgcca tgcccgtgat
     2161 catcggccga gttcgctatc ccgggatcaa gatccacgac tcgcgagcca tccgtctcct
     2221 ggaggttctc ctgcatggca gcacacaggt cggaggctgg accgtccggg aaatacacca
     2281 agccgtcctc acctcgttcg agctttccga ccatgtctac ggcatcaacc agctccgcta
     2341 cgatttgcgc aagctcaagg ggcacggtct gatcgagcgc gacacttccc gttatgccta
     2401 tcgcctcacc caccaaaagg gcaccgagac cgccttgctc ttcctcgtct tccataagcg
     2461 gctctgcggc cctctcgcca acagccgatt ccaccaccgc ccggaccctg gcgaccgccc
     2521 cgataccagg ctcgaagccg cctatcaccg cgccgacaag gccattgaca acattgtaca
     2581 actccttgag gcagcataat tttctcagaa tgtcgactcg ccttgtcaag gattcgacgc
     2641 gatagaatct acggtttgcc gtcagactcc cagca
//
LOCUS       DASRKK010000409         9862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_422184, whole genome shotgun sequence.
ACCESSION   DASRKK010000409 DASRKK010000000
VERSION     DASRKK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422184"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1993
                     /locus_tag="VFC15_09340"
     CDS             482..1993
                     /locus_tag="VFC15_09340"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZM10400.1"
                     /translation="MTVIKQDDLIQSVADALQYISYYHPVDFIQSLARAYEKEQSPAA
                     RDAIAQLLINSRMCAEGHRPICQDTGIVSVFLKVGMKVQWDATMSVEDMVNEGVRRAY
                     LHPDNKLRASVLADPAGERKNTKDNTPAVVNMTLVPGDKVDVIVAAKGGGSEAKSKFV
                     MLNPSDSIVEWVLKTVPTMGSGWCPPGIIGIGIGGTAEKAMLLAKEALMEPIDIQELI
                     ARGPKNRAEELRVELYEKINALGIGAQGLGGLTTVLDVKVKDYPTHAANLPVAMIPNC
                     AATRHAHFVLDGSGAVALHPPSLKDWPELTYNPAGARRVNLDTVTRDEVATWKTGEVI
                     LLSGKLLTGRDAAHKRMTEMLKRGEQLPVDFTNRFIYYVGPVDPVRNEVVGPAGPTTA
                     SRMDKFTRQMLKETGLLGMVGKSERGPEGIAAIRDHKAVYLMAVGGAAYLVSKAIKKS
                     RVLGFADLGMEAIYEFEVEDMPVTVAVDVNGNSVHESGPKEWQAKIAELVEIG"
     gene            2115..2996
                     /locus_tag="VFC15_09345"
     CDS             2115..2996
                     /locus_tag="VFC15_09345"
                     /inference="COORDINATES: protein motif:HMM:NF018400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HZM10401.1"
                     /translation="MELQEILDSLDRDTGRYPRAAVTVAIARRDEIVPALLATLEDVA
                     RDPVPYTSEMRWIHVFAMYLLAQFREVRAYPLLVRIFSVPGEFAFDLVGDTVTEDLGQ
                     ILASVSGGDISGMAALVENEAANEYVRAAGLTGLLTLCACGTLSRNEVMSYFKSLFQK
                     LRRTPDGVWSWLADSCADLCPAEVMEELRKAYEENLPNPGVIGWDDVEEALALGQEAA
                     MIKLSRSRHLVSDVHKELSWWYCFNKDQPAAVSDRPLPSPKFDVIDPIHRTEPKVGRN
                     DPCPCGSGKKYKKCCSR"
     gene            3046..4488
                     /locus_tag="VFC15_09350"
     CDS             3046..4488
                     /locus_tag="VFC15_09350"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZM10402.1"
                     /translation="MPAKPALRTEKDSLGYKEIPADVYYGIQTARAVENFPISGLRTH
                     PSLIRAIAMVKEAAALANRELGLVDEKTANAIIEAAKEVQQGKWDQEFVVDVFQAGAG
                     VSFHMNSNEVIANRASELLGGNLGEYKHVHPNDHVNYGQSTNDVFPTAMRLSTLLELE
                     KLYAVLDGLVAALDEKGKEFHHVLKSGRTHMMDAVPMRLGQEFHAYAGAIKRAKRSIG
                     QQSELLREVGLGGSAVGTGINTHPDYREKAIANLARISGQKLHVVDDMRYAMQSNLAM
                     SSVSSALRNLALEVIRVSNDLRLLSSGPNTGLAEINLPALQPGSSIMPGKINPVMAEL
                     AAMVSFQVVGYDTTVALAVQAGQLELNVMMPTMAYSVLTSITIMTNMLRQLTEFCIKG
                     ITANEKRTDYYVQSTVSLATALNPYIGYAKAAEIAKESVKTGKSIIEIARERGELTDA
                     QIKEILDPARMTEPVRPLEAAKEREKIKAK"
     gene            complement(4735..5139)
                     /locus_tag="VFC15_09355"
     CDS             complement(4735..5139)
                     /locus_tag="VFC15_09355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM10403.1"
                     /translation="MEVLIEHLGAVQFEIKARQHTLICDQPLDSKGDDEGMTPPELLL
                     ASLGSCAAYYAVDYLNRNQLSSEGTTVRVTAEKVKAPFRLDNFKIEVDVTAELNDQHL
                     KGIEEAVHRCLIHNTLLHPPQMKVEIKAPVAV"
     gene            complement(5334..6146)
                     /locus_tag="VFC15_09360"
     CDS             complement(5334..6146)
                     /locus_tag="VFC15_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10404.1"
                     /translation="MLFRGDSFDYDLRVDAIETRNGTTVYIGAAVANREAVEPPGLVG
                     PRPSFEISGSSITLYPSPGWTAELPVYRFADAAPREPLTAENVSKYIGEPPKRAVRSV
                     SVTIGETLGSVLHVSDVFRFENHIGGGFFYYLVRNGALVLAAGTLHGRIELETDFAVA
                     PEYEPDPDEPLPKEFMDLGGWRIPIGRIRTRKRYVTAQIKEQRFELVDGQAAVWNSCC
                     VFLARSNNALELVMGGPSSIAVFAAARLNDQVTEEIVSRAAHHMLQPASRLL"
     gene            complement(6195..7193)
                     /locus_tag="VFC15_09365"
     CDS             complement(6195..7193)
                     /locus_tag="VFC15_09365"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZM10405.1"
                     /translation="MPNSRRAKITALGTYVPPRVLTNKELEKMVDTTDQWILDRTGIR
                     ERHLVDKGVAASDLAVEAVKKLVQSHPFDLQEVDLIVVGTVTPDMMYPSTACLVQHKL
                     GIARTWGFDVSAGCSGFVYALNAGTQFIESGRYKKVLVIGADVNSSMTDFTDRAVCII
                     FGDGAGAVLLEPAEEGEEGIIDYEAQIEGMGGQYLYMPGGGSLNPTSHETVDQKMHYI
                     HQDGQQVFKYAVKKMSEMTERVLRKNGLTGQDVDCFIAHQANMRIITATADRLKMPIE
                     KVIINIDRYGNTTAGTIPLAMQTAIDEGKVKKGDLVLLAAVGAGFTSGATLLKWAY"
     gene            7350..7586
                     /locus_tag="VFC15_09370"
     CDS             7350..7586
                     /locus_tag="VFC15_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10406.1"
                     /translation="MVSNIQLLIAIGLPMLALAFGLYLNDRRFEGLNTRIDDLRTDLG
                     GRITRVGTSIDNLTSKLVDLDNRVVKIETKLGIG"
     gene            complement(7704..7801)
                     /gene="ffs"
                     /locus_tag="VFC15_09375"
     ncRNA           complement(7704..7801)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFC15_09375"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7919..9454
                     /gene="rimO"
                     /locus_tag="VFC15_09380"
     CDS             7919..9454
                     /gene="rimO"
                     /locus_tag="VFC15_09380"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZM10407.1"
                     /translation="MQLKTDSVALLDIPCTPTRANASRVDESGPDSSPMAAPKKIGFV
                     SLGCPKNLVDSEVMMGLLAAGGAQITTRAEDADVIVVNTCSFIESAKQESVDAILEMA
                     QHKTAGRAQKLIVAGCLVERYRNEIQKDIPEVDAVVGTGELQQILSAAGIASPSPAGS
                     PFVILNSRSEGDAREAAGRFSKGDWDGAIADLPNYLYDENTPRLLATPRYSAYIKIAE
                     GCDHPCSFCIIPQLRGKFRSRRFESVIAEAERLAEQGVREITLIGQDTTCYGEDLGLK
                     DGLALLLERLAQIEKLRWVRFLYAYPNKITGKLLQTIAAHEKICSYIDVPLQHASPDV
                     LKRMKRGAGAEIFLRSVAKMRREIPGVTLRTSFIVGFPGESAEDFEQLCQFVHEAQFD
                     WLGVFGYSDEEGAGAFALADKVPRREIERRRKKLMSMQKQISRKKKRALVGQQFDLLL
                     SGPSAETELLWEGRTAMHAPEIDGTVYVNDFGDREDVREGEFYRCEITEAHDYDLVAR
                     IVE"
BASE COUNT         2148 a         2778 c         2905 g         2031 t
ORIGIN      
        1 cgacgtccgc aatccgcgcg aatggggaca gcgcgccgtg agcctgcacg tttactcacg
       61 cccgttcgac tcctgcattg tctattcggt agaacaaggc agctgcgggg agattggcct
      121 gaactatacg tcgatgtacg gaaagctggt ttgaaagcag cactcagcac tcagcactta
      181 gcattcagca ctcagcactc agaatagcca agaaggaact gcaacaaagg acaaagacgc
      241 agggtctgta ggggtctcgt tttcgcggaa tatcctgttg gcccactgct gacggctgaa
      301 tgctgagtgc tgactgctga ctgctgagtg ctagatgcag ttcaacgcca tctcccacgt
      361 ttggggaatg ccgattcctc cgcgcacatt gaattctcta ggtatcatgc atccggcttt
      421 ctataattaa catgtgggcc tttgcccgga ctcttacgtt ctttcctaca gcgaggcagt
      481 aatgacagtc atcaagcaag acgatcttat ccagagcgtt gccgacgctc tgcaatacat
      541 ttcctactac catccggtcg atttcattca aagcctggcg cgcgcgtacg agaaagaaca
      601 gtcgcccgcc gctcgcgatg ccattgctca gttgctgatc aactcccgca tgtgcgccga
      661 aggccaccgg cccatctgtc aggacaccgg aatcgtgtcc gttttcctga aggtcggcat
      721 gaaagtgcag tgggacgcga ccatgagcgt ggaggacatg gtcaacgagg gcgtacgccg
      781 cgcgtatctc caccccgaca acaagctgcg cgccagcgtg ctggccgatc cggccggcga
      841 acgcaagaac accaaggaca atacgccggc ggtcgtgaat atgacgcttg ttcccggcga
      901 caaggttgac gttatcgtgg ccgcaaaggg cggcggctcg gaagctaagt cgaagttcgt
      961 catgctgaat ccttccgact ccatcgtcga atgggtgttg aagacagtcc ccacgatggg
     1021 ctcaggctgg tgtcctccgg gaattatcgg catcggcatc ggcggcacgg ccgagaaagc
     1081 catgctgctg gccaaggaag ccctgatgga gccgattgac atccaggagc tgattgcacg
     1141 cgggccgaag aaccgcgccg aggagttgcg cgtcgagctg tatgaaaaga tcaatgcgct
     1201 gggcatcggc gcgcagggac tgggcggcct gactacggtg cttgacgtaa aggtgaagga
     1261 ctatcccacg cacgccgcca acctgcccgt agcgatgatc ccgaattgcg cggccacacg
     1321 ccatgctcac tttgttctcg acggctcggg cgcggtggcg ttgcatccgc cgtcgctgaa
     1381 ggattggccg gagttgacct acaatccggc gggcgcgcga cgcgtgaacc tggacacggt
     1441 cacgcgcgac gaagtggcga cgtggaagac tggcgaggtg attctcctta gcggcaagtt
     1501 gctcaccggt cgcgatgccg ctcacaagcg catgacggag atgctcaagc gcggcgaaca
     1561 gcttccggtg gatttcacta atcgcttcat ctactatgtt ggcccggtcg atccggtgag
     1621 gaatgaagtg gttggcccag caggtccgac aaccgcctca cgtatggaca aattcacgcg
     1681 ccagatgctg aaggagactg gcctgctcgg catggtgggc aagtcggaac gcggcccgga
     1741 aggcatcgca gccatccgcg accacaaagc ggtttacctg atggcggttg gcggcgccgc
     1801 ttacctggtc tcgaaggcca tcaaaaagtc gcgggtgctg ggcttcgccg atcttggcat
     1861 ggaagccatc tacgaattcg aggtcgagga catgccggtc acggttgcgg tggatgtgaa
     1921 cggaaattcc gtccacgagt ccggtccgaa agaatggcag gcgaagatcg cggagttggt
     1981 ggagatcgga taacgctctg tcttcgactg taagagccgc tggccaccac cccagcggct
     2041 tttgatttcg gaccacatga gcgggccggg gacgtaaacg aacggctacg agcgcgtgct
     2101 aacctgacac tgcgatggaa ctgcaagaaa tcctggattc cctagatcgt gacaccggcc
     2161 gctatcctag agcggctgtt acggtggcca ttgcccgccg cgacgaaatc gtccccgcac
     2221 ttctggcgac tctcgaagac gtcgcgcgtg accccgtgcc gtataccagc gagatgcgtt
     2281 ggattcacgt ttttgccatg tacctcctgg cacagtttcg cgaggttcgc gcctacccgc
     2341 tgctggtgag aatcttctcc gtgcccggcg aattcgcgtt tgacctcgtt ggcgacacgg
     2401 ttacggagga cttgggccag attctagcct ctgtgtctgg cggcgatatc agcggcatgg
     2461 cggcactggt ggaaaacgag gccgcgaacg aatacgtgag agcagcgggt ctgacgggac
     2521 tgttgacgct gtgcgcttgc ggcacactca gccgcaacga agtcatgagc tacttcaaga
     2581 gcctctttca aaaactgaga cgcaccccag atggcgtatg gagttggttg gccgacagtt
     2641 gcgccgatct ttgtcccgcg gaggtcatgg aggagcttcg gaaggcgtat gaagaaaatc
     2701 ttcccaatcc cggtgtgatt ggctgggatg atgtggaaga ggcgttggcg ctgggtcagg
     2761 aagcagcgat gatcaagctt agccgtagcc gccacctggt ctcagatgtg cacaaagagc
     2821 tgtcgtggtg gtattgcttc aacaaggacc aacctgcggc agtctccgac agacccctcc
     2881 cgtctccgaa gtttgatgtc atcgatccga tccatagaac ggaacccaag gtcggacgca
     2941 acgatccctg tccgtgcggc agcggcaaga aatacaagaa gtgttgcagc aggtagatga
     3001 tgctcatcag gtctcgcgag catctcttcc gtagaggatg aattcatgcc agccaagcca
     3061 gcgcttcgca ccgaaaaaga ctctctcggc tataaagaaa tccctgccga cgtttactac
     3121 ggcattcaaa ccgcccgcgc ggtggagaac tttcccatca gcggattgcg cacccatcct
     3181 tcgcttatcc gtgctatcgc gatggtgaag gaagccgcag ccctcgctaa tcgcgagctc
     3241 ggtctggtag acgagaagac ggccaatgcc atcatcgagg cggccaagga agttcagcag
     3301 ggcaagtggg accaggaatt cgtcgtcgac gttttccagg cgggcgcggg cgtcagcttc
     3361 cacatgaaca gcaacgaggt catcgccaac cgcgcgagcg agctgctggg cggcaacctg
     3421 ggcgagtaca agcacgttca tcccaacgat cacgtcaact acgggcaatc gacgaacgac
     3481 gtcttcccca ccgcaatgcg cctctccact ctgctggaac tggaaaagct gtatgcggtg
     3541 ctcgatggct tggttgccgc gctcgacgaa aaaggcaaag agttccacca tgtcctgaag
     3601 tcgggccgga cccacatgat ggatgcggtg cccatgcggc tgggccagga gtttcacgcc
     3661 tacgcgggcg cgatcaagcg cgcgaagagg tcaatcgggc aacaatcgga gctattgcgc
     3721 gaggttggcc tcggcggctc agccgtgggc accggcatca acacccatcc cgattatcgc
     3781 gagaaggcga tcgccaacct ggcgcgcatc tcgggacaaa agctgcacgt ggtggacgac
     3841 atgcgctatg ccatgcaatc caacctggcg atgtcgtcgg tcagttcggc actgcgcaac
     3901 ctggcactgg aggtcatccg cgtctccaac gatctgcggc tgctctcgtc gggcccgaac
     3961 acgggactgg cggaaatcaa cctgcccgcg ctacagccag gctcttccat catgccgggc
     4021 aagatcaatc cggtaatggc ggaactggct gcgatggttt cgtttcaggt tgtcggctat
     4081 gacaccacgg ttgcgctggc ggttcaggcc ggccagctcg aactcaacgt catgatgcca
     4141 accatggcct acagcgtgct cacttcgatc accatcatga ccaacatgct gcggcagctc
     4201 accgagttct gcatcaaggg catcacggcc aatgagaaac gcactgacta ctacgtgcag
     4261 agcacggtgt cgctggcaac ggcgctgaac ccatacatcg gttatgccaa agcggcggag
     4321 attgcgaagg agtcggtgaa aaccggcaag tccatcatcg agattgcacg tgagcgcggc
     4381 gagctgaccg acgcgcagat caaggagatt ctcgaccccg cgcgcatgac ggagccggtg
     4441 cgtccgctgg aggcggcgaa ggaaagggag aagatcaagg cgaagtaggt gacggattcg
     4501 attgtctgcg gccaggtgga aacgtgaggg gacgagaacg attccagcga tatgaagagc
     4561 aaacgccccg ccctagccaa aaccaggcta gaacggggca tccaagcgcc gagcggctca
     4621 cggacaactt cgcgggtcac attgggacgt gacaacggca ggagcaaaac tcccacccaa
     4681 gcaaaggaag cttgggtggg gcacccgcac ccggcgacac atttgcgaaa caagttacac
     4741 agccactggc gctttgatct ccactttcat ctgcggcgga tgcagcaagg tgttgtggat
     4801 caggcaacga tgcacagctt cctcgatacc ttttaagtgc tggtcgttga gttcagccgt
     4861 cacatcgact tcgatcttga agttgtctag ccggaacggc gctttgactt tttcagccgt
     4921 gacccgaacg gtcgtccctt ctgatgaaag ttggttgcgg ttcagatagt ccacggcgta
     4981 gtacgcggcg cacgatccca gagatgcgag cagcaactcc ggaggcgtca taccttcgtc
     5041 atcgcccttg ctgtccagcg gctgatcgca aatcagtgtg tgctgccgcg ccttgatctc
     5101 gaactgcact gccccgagat gttcaataag aacttccatg atgcctcctg ctctaagatt
     5161 gctaagattg ctgtgaatcc gtaatcgtat ttgccggcgg aaggagtgcc gcatccatgt
     5221 accggcgcat gttggcggag acaagagttg agtaagaact tgaattccgt ggtgacagca
     5281 aagaaatgag ctcgaccctg cctgccgcaa cgacaatcgt tgcccagtca ttctcagagg
     5341 agtcgcgacg cgggttgcaa catgtgatga gccgcgcggg aaacaatttc ttctgttacc
     5401 tggtcattta atcgtgccgc tgcaaaaact gcgatggaag agggcccccc catcaccaac
     5461 tccaaagcgt tattggaacg ggcaaggaaa acacaacagg aattccaaac agctgcttgt
     5521 ccatcaacca actcgaatct ttgctccttg atttgcgctg ttacgtagcg cttccgtgtc
     5581 cgtattcggc caataggtat gcgccagcca cctaagtcca taaattcttt cgggagcggc
     5641 tcatccgggt cgggctcata ctctggcgcc actgcaaaat ccgtttcgag ctctatccgg
     5701 ccatggagtg ttcctgccgc caacacaagc gcgccgttgc gaacgaggta atagaaaaag
     5761 cctccgccga tatgattctc gaagcgaaag acgtcgctca catggaggac agaaccgagt
     5821 gtttcgccaa tggtcacaga gaccgaccga acggcgcgct ttggaggctc tccaatgtat
     5881 ttacttacat tttcagcggt gagcggctcg cgtggagcag cgtcggcgaa ccggtaaaca
     5941 ggaagttccg cagtccatcc tggcgatgga taaagggtga tagagcttcc ggaaatttcg
     6001 aaggatggcc tcggcccgac cagaccggga ggctccacag cctcacggtt cgcaactgcc
     6061 gcgccgatgt agaccgttgt cccattcctg gtctcgatcg catcgacccg cagatcgtag
     6121 tcgaaactgt cgccccggaa gagcatgatg cggtcattat gtcacgcgta ctctttgaag
     6181 attacttccg cacgtcagta tgcccatttg agcagcgtcg ctccagaagt gaatccggcg
     6241 cctacggctg ccaataaaac caagtcgccc ttcttcacct tgccttcgtc aatcgcggtc
     6301 tgcatggcga gcgggatggt tcctgccgtg gtgttgccgt agcggtcaat gttaatgatg
     6361 accttctcga tcggcatctt aagccggtcc gcagtcgctg tgatgatgcg catgtttgcc
     6421 tggtgggcga tgaagcaatc cacgtcctgt ccggtgaggc cgttcttccg caagacgcgc
     6481 tctgtcatct ccgacatttt cttcaccgca tacttgaata cctgttgtcc atcctgatga
     6541 atataatgca ttttctggtc gacagtttca tgtgacgtag ggttcaaact cccaccgccc
     6601 ggcatgtaca ggtactggcc acccatgcct tcgatctggg cctcgtagtc gataattccc
     6661 tcctcaccct cttccgctgg ttccagcagc acggcgcccg ctccgtcgcc gaagatgatg
     6721 cacaccgcgc gatcggtgaa gtcagtcatg gaagagttca cgtccgcgcc gatcaccagc
     6781 accttcttgt accggccgga ctcgatgaac tgggtacccg cgttcagcgc gtacacgaac
     6841 cccgagcatc ccgcggacac gtcgaagccc caggtacgcg cgataccaag tttgtgttgc
     6901 accaggcacg ccgtcgaggg gtacatcatg tcaggggtga ccgtccccac cacgatcaag
     6961 tccacttctt gaagatcgaa agggtgcgat tggacgagct tcttgaccgc ttcgaccgcc
     7021 aagtcgctgg ccgctacgcc tttgtccacc aagtggcgtt cgcggattcc ggtacggtcc
     7081 aggatccact ggtcggtggt gtccaccatt ttttccagtt ccttgttggt gagaacgcga
     7141 ggcgggacgt aggtacccag cgcggtaatt ttggcgcgac gcgagtttgg caatcgagtt
     7201 ctccgggaca gcagttacga atgaaccttc attttcaatg atacaaaggc gaaagtctat
     7261 gtggagcaaa gtggtgccaa tcgagcaatt tattttggcg gttcgctttg ctgagaggcg
     7321 gtagcgctgg gcgagaggag tagaatcgtt tggtgagtaa catccaactg ctcatcgcca
     7381 tcggattgcc catgctggcc ctcgccttcg gtttgtacct aaacgatcgg cgtttcgagg
     7441 gactcaacac ccgcattgac gatctgcgca ccgatcttgg cggacgcatc actcgcgtcg
     7501 gaacttcgat agacaatctc actagcaagc tcgtggattt ggacaaccgc gtggtcaaga
     7561 ttgaaacgaa acttggcatc gggtaagggt ggctattagc ttctagctcg tggcttttag
     7621 cttttacgcc tttagcaagc cgaattcgct gctggctgct aagagctaat ggctaatggc
     7681 taagagccaa tagccagaaa agccaggcgc cagttgaccg ctgcacccgc actagcctgc
     7741 taccgttgct tccttccgga cctggcggga ttcacaagat tgcgttgcgc gggaccagcg
     7801 cccgaccatt taatataaca tccccctcgc gacggcgtgc tggacgcggg aagggcgcga
     7861 gttgggacct cgcctgctct gggagccgcg aaaaacaatg gtaaaatagg ggaattgcat
     7921 gcagctaaaa accgattctg tagccctgct ggacattcct tgcactccaa cccgcgccaa
     7981 tgcgtcgcgc gttgacgagt ccggacctga ttccagcccc atggctgctc ccaagaaaat
     8041 tggattcgtg agcctgggct gtcccaagaa cctggtggac agcgaggtga tgatgggcct
     8101 tctcgccgcg ggcggggctc agatcaccac ccgcgcggaa gatgccgacg tcatcgtcgt
     8161 gaatacttgc tccttcattg agtcggccaa gcaggaatcg gtggatgcga tcctggaaat
     8221 ggcgcagcac aagaccgcag gacgggcgca aaagctcatc gtggctggct gcctggtaga
     8281 acgctatcgc aacgagatcc agaaggacat tcccgaagtt gacgccgtcg tgggtactgg
     8341 cgagctacag cagattctca gcgcggcggg aattgcatcg ccgtcgcccg ccggttcccc
     8401 gtttgtgatc ttgaactcgc gttccgaggg tgatgcccgt gaggctgcgg gcaggttctc
     8461 gaaaggagat tgggacggcg cgattgccga cttgcccaac tatttatacg acgagaacac
     8521 gccgcgcctg ctggcgactc ccaggtattc ggcttacatc aagatcgccg aaggctgcga
     8581 tcatccctgc tcgttttgca tcatcccgca attgcgcggg aagttccgtt cgcggcgatt
     8641 cgaatcggtg atcgccgagg cggagcgtct tgcggagcaa ggggtgcgtg agattacgtt
     8701 gatcgggcag gataccacct gctacggaga agatctgggc ctgaaagatg gtctggcgct
     8761 gctgctggag cggctggctc aaatcgagaa attgcgctgg gtgcgcttcc tctacgccta
     8821 tcccaacaag atcaccggca agctgctgca aacgattgca gcgcatgaaa agatttgctc
     8881 ttatattgac gtgccgttgc agcacgcctc gcctgatgtc ttgaagcgca tgaagcgcgg
     8941 cgctggcgcg gagatatttt tgcgctcggt ggcaaagatg cgacgcgaga ttcctggtgt
     9001 gaccctgcgt acctcgttca tcgtgggctt ccctggcgaa agtgcggagg atttcgaaca
     9061 gctctgccag ttcgtgcacg aggcgcaatt cgactggctg ggagtgtttg gctactccga
     9121 cgaggagggg gcaggggcgt ttgccctggc agacaaagtc ccccggagag agatcgagag
     9181 gcgccgcaag aagcttatgt ccatgcagaa gcagatcagc cgcaagaaga agcgtgctct
     9241 ggtgggacaa cagttcgatt tgcttctctc gggtccctca gcagaaacag agttgctgtg
     9301 ggaaggccgg acagcgatgc atgctcccga gattgatggc acggtttacg tgaacgattt
     9361 cggcgatcgc gaagacgttc gcgagggcga gttctaccgt tgcgagatca cggaagcgca
     9421 cgactacgat ctggtggcgc gcattgtgga atgaggaatg ccgtcagcaa tcagcattca
     9481 gccaagccgc ggttgcgact gccacttcgt tatacagatt tcgctgctat gttctgggta
     9541 gagaagagac atttgttaga tcgttcgcaa aacggcaaaa cagcagctcg catcaaatcc
     9601 gtctggccaa atgccaagtg ctaactgcca ggggagtggc tgagtgctga acgctgagtg
     9661 ctgaacgctg agtgctgaac gctgaatgct gattgctagc catgacgaag aaacttgttc
     9721 acagcttacg ttgtccaact tgcagaaaga tcgtgctggc cagcgatctt gagtatcctt
     9781 tctgcagcga acggtgccgt gcgattgacc tcggcaagtg ggcgagcggc ggctatgtca
     9841 tctccaccgc gttgaacgat cc
//
LOCUS       DASRKK010000410         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1301857, whole genome shotgun sequence.
ACCESSION   DASRKK010000410 DASRKK010000000
VERSION     DASRKK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301857"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..617
                     /locus_tag="VFC15_09385"
     CDS             273..617
                     /locus_tag="VFC15_09385"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM10408.1"
                     /translation="MPTDPELDLRGRLIRSLGALREMLPGSFVERTRKCGKPNCRCAD
                     GKQLHREFLLSVVFEGKPQTVHLPAALVEEARLKVEMRERFDEAAGKIAHLNLRRFLR
                     QKEKHRRGSPPV"
     gene            598..1851
                     /locus_tag="VFC15_09390"
     CDS             598..1851
                     /locus_tag="VFC15_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10409.1"
                     /translation="MVRRQFESYLDKVFGFADLVAALPEGRTYPLHPWRKVFDTVFLG
                     SACQLATAHRIEFECRAGVLSKRIGPLSEDTLGYAMQLQSPQSIFELGCTVAKRLKRN
                     GVLGSEWARGRVVAAVDGIEICNSYVRCCETCLERKVERKVDGEILECIQYYHRVSAV
                     TLVSTPFPVFLGIRFQKPGETEVAASLDLLQQLVNQLGRRFIDVLVADAIYLQTPFVR
                     AIELLGLEWVITLKDNQPTLLAEAERLTAVPADCQWSAGKDDFSLWHLPEVDWPVAGR
                     MVRVVKTVRVQRVKRVKIEKIGGLRAKRQEEVSVDSTNFYATNVNLGAIPPQFIQQLG
                     RSRWTIDAAAFQTLSTQCHLKQPSVHQDVALVVLTMIRVLAYTLMMVFYHRQIRSHCR
                     KAPPSFCELARSLALALFTPGADTS"
     gene            2137..>2325
                     /locus_tag="VFC15_09395"
     CDS             2137..>2325
                     /locus_tag="VFC15_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM10410.1"
                     /translation="METISVKVEKSGRILIPAAIRRQLKLEAGTEVILRVDETGIQMG
                     TREQALARIRKELRRYIPE"
BASE COUNT          472 a          711 c          628 g          514 t
ORIGIN      
        1 ccggtgttgg tcccaagggc gatctttcgg actgcattcg gagaggtatt ccggctcggc
       61 ggtcgccggt tcatcagatt gatcgaccgc cggaactacc gtcgcacacc caaacccgca
      121 gcgttttcgt aacgttactt ctatcgtgat tccaacccat tgaattggaa gaagtacgtg
      181 cccgtaagtg cccgctgaac tcccccggtc attcttgcaa atcttcgaag ggccttgact
      241 tccgtcgatt ctgacactat tattagtggc agatgccgac cgaccctgaa ctcgacctcc
      301 gcggcaggct cattcgtagt ttgggcgcct tgcgcgagat gttgcccggt tcctttgtcg
      361 agcgcacccg taagtgcggc aagcccaact gccgctgcgc cgatggcaaa cagctccatc
      421 gcgagtttct cctctcggta gtgttcgagg gtaagccgca aaccgttcat ctgcccgccg
      481 ctctggtcga ggaagcgcgt ctcaaggtcg aaatgcgcga gcgctttgat gaggccgcag
      541 gcaagatcgc ccatttgaac ctccgccggt ttctccgcca gaaggagaag caccgccgtg
      601 gttcgccgcc agtttgagtc ctatctcgac aaggtgtttg gtttcgccga tctggtagcc
      661 gccctacccg agggcagaac ctaccccctc catccttgga gaaaagtctt cgacaccgtg
      721 ttcctgggat cggcctgcca gttagcgaca gcgcatcgca ttgaattcga gtgccgtgcc
      781 ggcgtccttt ccaagcgcat cggaccattg agcgaagaca ctctcggcta cgccatgcag
      841 cttcagtccc ctcagtcgat atttgagttg ggttgcacgg tagccaaacg cttgaagagg
      901 aacggcgtcc tcggttcgga gtgggcacgg gggcgcgtgg tcgccgccgt cgatggcata
      961 gagatctgca actcctacgt gcgctgttgt gaaacttgcc tggagcggaa ggtcgaaagg
     1021 aaggtagacg gcgaaatcct cgagtgcatt cagtattacc accgcgtctc ggccgtcacg
     1081 ctggtcagca cgccttttcc ggtattcctg ggaatccgct ttcagaaacc cggcgaaacc
     1141 gaagtggctg cctctctgga cttgctgcaa caactggtta accaactcgg gcgccggttc
     1201 atcgacgtgc tagtcgctga cgccatctat ctgcagacgc cgttcgtccg tgcgatcgaa
     1261 ttgttgggct tggaatgggt cattacgctg aaggacaatc aaccgacttt gctggccgag
     1321 gcggagcgcc tcaccgcggt ccccgccgat tgccagtggt ctgccggtaa ggacgacttt
     1381 tctctttggc atttgccgga agttgactgg ccggtggccg gccgcatggt gcgtgtcgtc
     1441 aaaaccgtcc gggttcaaag agtcaagcgc gtcaagattg agaaaatcgg cggacttcgc
     1501 gcgaagagac aagaagaggt ctcggtagac agcaccaact tctatgctac caacgtgaac
     1561 ctcggcgcca tccctccgca gttcattcag cagttgggcc gaagccgctg gaccattgac
     1621 gccgccgcat tccaaaccct ctctacccag tgccatctca agcagccctc cgtccatcaa
     1681 gacgtcgcac tggtagtgct gacgatgatt cgggttctgg cctatacctt gatgatggtc
     1741 ttttatcatc gccagattcg aagccactgc cggaaggcgc cgcccagttt ttgtgagcta
     1801 gccagatcct tggcgctggc gttgttcacg cctggcgccg acaccagtta atctgcgcat
     1861 gaccgtccgg ccgtaactgc ctgctcctcc attccgcgcc cgatcgccag gtgccatgct
     1921 ctccattgcc cccagccaag gtatccctgc ctccgtcaac ctctccgcgc ctggaattgc
     1981 ctctgccgac cctcaaaacc ccaattcaaa aatggtccgt ccttcactca acccgtaaac
     2041 tccacaaaag cagtcggatc gcccctcctc cgaactcacg ttacgaaatc gctggttaac
     2101 agccctggac tactcagatg atgataaaat tgccgtatgg agacaatttc ggttaaagtg
     2161 gagaaatctg gcagaatctt gattcctgcc gcgatccgtc ggcaactcaa actggaagca
     2221 ggcactgagg taattcttcg ggtagatgag actggcatcc aaatgggcac gcgtgaacag
     2281 gcgctcgctc gcattcgcaa ggaactgcgc cgctatattc cagag
//
LOCUS       DASRKK010000411         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_911190, whole genome shotgun sequence.
ACCESSION   DASRKK010000411 DASRKK010000000
VERSION     DASRKK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911190"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..529)
                     /locus_tag="VFC15_09400"
     CDS             complement(62..529)
                     /locus_tag="VFC15_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10411.1"
                     /translation="MGLRDKKRHPGWLQPRWERQKRDTAARVEAAITALQKDHAEVTY
                     ASICKRVSILFGLSISPNTIKRNELSYRIYVASRRPPRRKQLRESVLMQLVNSASGPE
                     KRSMWSKTARLRRESKDSLIARLISLERRVRQQQSAENLLREEILRLAQAGSR"
     gene            complement(513..2885)
                     /locus_tag="VFC15_09405"
     CDS             complement(513..2885)
                     /locus_tag="VFC15_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10412.1"
                     /translation="MTSNDIALRIQADLGLTWPRNRSLPNEVVAEICEALWQEGISPN
                     RRIVQQRMPVWNDHAFGPGIVAWRRKKGLPEQGTHVRCALPSNLRELAKIISPAMASA
                     PLTSYDPAGDGRWQVPSVKVLSYLGRIENPSVRDAMALFALLRSDRQQKSIYNQIANF
                     VRVLRRVMIEQSIEDITSVDANDLLFRIHVGEIGKGLSEHQRRTFLGQWSAVRNAFDE
                     YAERLTPKQLEVMKNFFIGLVTDRRRFMRHKTYSAYLREQQERVKIKTDAVQSKFYQI
                     RHVAGIRCNQLRRLYEATRQAVAAVEANDLRCPYEFSYEETAQTMGRRAVRQRVELTL
                     WDSNSLREHAVALGYHEAPATTLQRRWQEASFSPKNRAYFVEYNATTSLTQGCPAEPF
                     WFLDLFRHRVFRHLDVRSDSDLAERREAFHAKWGYESKSAWADGGLLAFGPTDFRPID
                     FLQQREGHEFLPYEGIYAACMFGGLVIRMGTITGARIGEIMQIAQSPDCFKQLENVGP
                     KAATRWMLRLVPKGRQKREDYFIDEDTKRHLVELIRFQCEKWGGPLIPIVKIEDDKRA
                     PDRYVLQWNGRGLRLHVLNTFMRFLLHGLILRSLDGNVVQLSSHLLRHSFATEMADLN
                     TPVEVIAKLLHQRDLTVTKYYARPTPTKVMSAAEMIFVDRIDLGAEGRRSPDEIGRML
                     KEAEGKVGALTEVFGGTCVVANMCPAKFVCVGCAGNAPDPAKRYQIDQKQAWAEQQLL
                     YARREKLVAEERQMKQLIADCKLIKEELDLIETVRKDAAQIVSVEHGSPR"
     gene            complement(2882..>3418)
                     /locus_tag="VFC15_09410"
     CDS             complement(2882..>3418)
                     /locus_tag="VFC15_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10413.1"
                     /translation="RYFDDDANGRRAHDRQGLCLADLGKMDAADLAKVRLFLTTRGGP
                     VSPRMFRSDYWNPALRAAGIAAPPHIARHWFVTNALRTIERTATDQNEMVRRKAELVQ
                     YMAWKTAERTLRAYEHVVRDSDFIDTTLSSIHKAMKQREQQVKKDPGLLAQFATVQSL
                     DVSKALDEDIAILTGALA"
BASE COUNT          657 a         1079 c          922 g          760 t
ORIGIN      
        1 gaaacttccc gtccggcggc aaaccatccg ctggggtgct tcagctttcc ggctggtgcg
       61 gtcaccgcga accagcctgc gccagacgca gaatctcttc gcgtagcaga ttctccgcgc
      121 tctgctgctg cctcacccta cgttccaggg agatgagtct ggcgatgagc gagtctttgg
      181 attcccggcg taaccgcgca gtcttggacc acatggatcg cttctccggg ccagatgcgc
      241 tgttgactag ctgcatgagg accgattccc gcagttgctt acgcctgggc ggccgccgac
      301 tcgccacata aatccgatag gagagttcgt ttctcttgat cgtgttggga gagatcgaaa
      361 gaccgaacag gatactcacg cgtttgcaaa tactcgcgta cgttacctcg gcatggtcct
      421 tctgcagtgc ggtaatggct gcttcaacgc gtgcggcggt atcgcgtttc tggcgttccc
      481 atcgcggctg cagccagccg ggatgccttt tcttatctcg gagacccatg ctcgacgctc
      541 acaatctgcg ctgcgtcttt ccgcaccgtt tcgatcagat ccagctcctc cttgatcagt
      601 ttgcagtcgg cgatcaactg cttcatctgc cgttcttccg ctacaagctt ttccctgcgc
      661 gcatacagta gctgttgttc cgcccaagcc tgcttttggt cgatctgata tcgcttcgct
      721 ggatccggcg cgttgccagc acagcctaca cagacgaact tggccgggca catgttggcc
      781 actacgcagg ttccgccaaa cacctccgtc agagctccga ccttgccttc agcctctttc
      841 aacattcttc cgatctcgtc ggggctgcgc cgcccctcgg cacccagatc gatgcgatcc
      901 acaaagatca tttctgctgc gctcatcacc ttcgtcggag tcggtcgcgc atagtacttc
      961 gtgacggtta ggtcgcgctg gtgaagcagt ttggcaatga cctcaaccgg cgtattcaag
     1021 tcagccattt cggtggcaaa cgaatgccgc agcaggtgcg aactaagctg aaccacattg
     1081 ccgtcgagtg agcgcaggat aaggccatgg agaaggaacc gcatgaacgt attcagaacg
     1141 tgcaagcgaa ggccgcggcc attccactgc agcacgtacc gatcgggcgc tcgcttgtcg
     1201 tcttctattt tcacgatggg aatcagggga ccaccccact tctcacattg aaaacgaatc
     1261 agctccacca ggtggcgttt ggtatcctcg tcgatgaaat agtcctcccg cttctgcctt
     1321 cctttcggca ccagccggag catccaccgc gtcgccgctt tggggcccac gttctcaagc
     1381 tgcttgaagc aatccgggct ttgcgcaatc tgcatgatct cgccgatgcg cgccccggta
     1441 atcgttccca tgcgaatcac cagcccgcca aacatgcagg ctgcgtagat accctcataa
     1501 ggaaggaact cgtgtccttc gcgctgttgc aggaagtcga tcggccggaa gtcggtcggg
     1561 ccgaacgcca gcagcccgcc gtccgcccag gcgctcttgc tttcgtatcc ccacttagca
     1621 tggaaggctt cgcgtctttc ggccagatcg ctgtcgctgc gcacatccag gtggcgaaac
     1681 acccgatgcc ggaaaagatc aaggaaccag aaaggctctg ctggacagcc ttgcgtaaga
     1741 cttgtggtgg cgttgtactc gacgaaatag gcgcgattct tcggcgagaa actggcttcc
     1801 tgccagcgcc tttgaagcgt ggtggcgggc gcctcgtgat aaccaagcgc caccgcgtgc
     1861 tcccgcaacg agttggagtc ccacagcgtc aactcaacgc gctgccgcac tgcccgccgg
     1921 cccatcgttt gcgccgtttc ttcatagctg aactcatacg ggcagcgaag atcgttggct
     1981 tcaacggctg cgacggcttg ccgagttgcc tcgtacaggc gacgcagctg gttgcagcgg
     2041 atccccgcta catgtcgtat ctgataaaac ttcgactgca ctgcgtcggt cttaatcttg
     2101 acccgctctt gctgttcgcg aagatacgcg ctgtaggtct tgtggcgcat gaaccttcgc
     2161 cgatcggtca ctagcccgat gaagaagttc ttcatcactt ccagttgctt cggcgttagt
     2221 cgctccgcat attcatcgaa cgcgttgcgc accgcactcc attgacccag aaacgtacgc
     2281 cgctggtgct ccgacaagcc cttcccgatc tcacctacat gaatacgaaa caacagatca
     2341 ttcgcatcga cgctggtaat gtcctcgatg gactgttcga tcatcacacg acgcagcacc
     2401 cggacgaagt tcgcaatctg gttgtagatg gacttctgct gccggtcaga tcgcaagagc
     2461 gcaaacagcg ccattgcatc ccgcacggac ggattttcaa tccggcccag gtaggacagg
     2521 acttttaccg aagggacttg ccagcgccca tcgcctgccg gatcgtagga ggtcaacggc
     2581 gcgctcgcca ttgcgggact gatgatcttt gccagttcgc gaagattgct aggtaaagcg
     2641 cagcgcacat gagttccttg ctcgggaagt ccttttttcc tcctccacgc cacgatgccg
     2701 gggccgaatg cgtggtcgtt ccacaccggc attcgctgtt gcacaatgcg acggttcgga
     2761 ctgatcccct cctgccacag cgcctcgcag atctccgcta caacttcgtt cggaagggac
     2821 ctgttcctgg gccacgttag tcccaagtcg gcctgaatgc ggagcgcgat gtcgttggag
     2881 gtcacgcaag tgctcccgtc agaatcgcga tgtcctcatc caacgctttg ctcacgtcaa
     2941 gggactggac agtcgcaaac tgcgccaaca ggcctggatc cttcttgacc tgttgctcac
     3001 gctgtttcat cgccttgtgg atggagctga gagtggtatc aatgaagtcg ctatcccgca
     3061 cgacgtgttc gtaggccctg agagtccgct cggccgtctt ccaggccatg tactgaacca
     3121 actccgcctt gcggcggacc atttcattct gatccgtcgc cgttcgctcg atggtccgaa
     3181 gcgcattggt gacgaaccag tgccgcgcga tatggggcgg agcagcgatc ccggccgccc
     3241 gcaacgcagg attccagtaa tcgcttcgaa acatccgcgg actcacggga cctccgcgcg
     3301 tggtcagaaa caatcggacc ttcgccagat cggcagcgtc cattttcccc agatccgcaa
     3361 ggcaaagccc ttgccggtcg tgcgcacgcc gcccgttcgc atcgtcatcg aagtatct
//
LOCUS       DASRKK010000412         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_520719, whole genome shotgun sequence.
ACCESSION   DASRKK010000412 DASRKK010000000
VERSION     DASRKK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520719"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFC15_09415"
     CDS             <1..358
                     /locus_tag="VFC15_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZM10414.1"
                     /translation="AQWLGWQAALTHWTIVLGDQPHLRPQTLGQVLDFSASHPTKVCQ
                     PARRGHGRHPVLLPRTVFRRLTRTKAATLKEFLVGRSQEAALCEVDDPGMEVDIDRPE
                     DYAKAVDLAAKRRAEK"
     gene            622..1755
                     /locus_tag="VFC15_09420"
     CDS             622..1755
                     /locus_tag="VFC15_09420"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZM10415.1"
                     /translation="MQDLNAADESWEILQQFLPDNLEESARERGAFQRARGEIQSAEV
                     LLRLLLLHVAGGLSLEQAVVRAREHKLVNISAVGLFKRLRSSGPWLVWLTEQLVKTLA
                     ADWDDALWKGRPVRVLDATDIEEPGPTGTDWRLHYSLRLPQLSCDFLELTDVHGGESL
                     KRLPVAKGDIVLCDRGYSHREGVAQVLGAGADVVVRLNTGAFPLTDGQDQGLDLVKLV
                     AKLKVGQLGEWPVWFKHAGQRHPLRLCVLRKSKVATQQAQRKAEENAHDKGQQVRPET
                     LKLAAFVLVLTSLPATWGTALVLELYRARWQVELAFKRLKQLLAAGHVPKTTDASSRA
                     WLQAKILTALLIEHMIHAGRFFSPWGYRLQPGAAVAGLQGSAG"
     gene            complement(1896..>2160)
                     /locus_tag="VFC15_09425"
     CDS             complement(1896..>2160)
                     /locus_tag="VFC15_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10416.1"
                     /translation="DTFQRLVAFLLDSPQRYHWFGRRFVVMPDHIHLIAHMGYDAVRL
                     GQWIKALKAVVGGLEPRQERPVGAPGLQDEASSDGVGRVPHKH"
BASE COUNT          407 a          594 c          729 g          430 t
ORIGIN      
        1 ggcgcaatgg ctgggctggc aggcggctct cactcactgg acgattgtgc tgggcgatca
       61 gccccatttg cggccccaga cattggggca ggttctcgac ttcagcgcct cccaccccac
      121 caaggtctgc cagcccgcgc ggcgcggtca cgggcgccat cccgtcctgc tgccccgaac
      181 cgtgttcagg cgactgacgc gcacaaaagc cgcgaccttg aaggagtttc tcgttggccg
      241 gtcccaggaa gccgccctct gcgaagtgga cgatcccggg atggaggtgg acatcgaccg
      301 gcccgaggat tacgccaagg cggttgacct ggccgccaaa cgccgcgccg agaagtgaga
      361 tgccgctccc ttgtcctggc ccaggaggat ggcgaatcgc ttgaagagcg aggcgggctt
      421 cgtggtgagg tctccggttt caatggggcg tcccggcgtc ggcagatgat ttcagggtaa
      481 agctgttttg cgtcgtggga ttttaggcgg cgcagttggg cgatatgatt cgtgaggtgt
      541 gagcatgtgc gcgccggggt tcccccataa gcattaacct aataattgac tgattgcggc
      601 ggctgcccta tccttggggg catgcaagac ctgaatgctg ccgatgagtc ctgggaaatt
      661 ctgcaacagt ttttgccgga taacctcgaa gagagtgcgc gcgagcgcgg tgcctttcag
      721 cgcgcccgcg gggagatcca gagcgccgag gtgctgctgc gactcctgtt gttgcatgtg
      781 gcgggtgggc tgtcgctgga acaggcggtc gtgcgggcac gggagcacaa gttggtgaat
      841 atcagcgcgg tggggttgtt caagcggctg cgcagttcgg ggccttggct ggtttggttg
      901 actgaacaat tggtgaagac gctcgcggcg gactgggatg atgcgctgtg gaaggggcgc
      961 ccggttcgcg tgctcgacgc tacagacatc gaagagcccg ggccgaccgg aacggattgg
     1021 cggttgcatt attcgctgcg gctgccgcag ttaagttgtg attttctgga actgacggac
     1081 gtgcatggag gtgagagttt gaagcggctg ccggtggcca agggcgacat tgtgctctgt
     1141 gatcgcggtt acagtcaccg ggagggcgtc gcccaagtgc tgggggcggg agccgatgtg
     1201 gtggtgcggc tcaacacggg ggcttttccg ctgaccgatg ggcaagatca gggcctcgat
     1261 ttggtgaagc tggtggcgaa attgaaagtc ggacagttgg gggaatggcc ggtctggttt
     1321 aagcacgcgg gtcagcgtca tccgctacgg ctgtgcgtgc tgcgcaagag taaggtggcg
     1381 acccagcaag cccaacgcaa ggctgaggag aatgcccacg acaagggcca gcaagtccga
     1441 cctgagacgc tcaaactggc cgcctttgtc ttggtgttaa cttccttgcc ggccacttgg
     1501 ggaaccgcgc tggtcttgga actctaccgt gcccgctggc aggtggagtt ggcgttcaaa
     1561 cggctcaaac aactcttagc cgccggccat gtgcccaaga cgaccgatgc atccagccgc
     1621 gcctggctgc aagccaagat tctgacggct ttgctcatcg agcacatgat tcatgctggg
     1681 cgcttttttt ccccctgggg ttaccggcta cagccaggag cggcggtggc aggtcttcag
     1741 ggaagtgcgg gatagtgtga tcagtgtgct gcaaatgccg ctcagcttgc gacacctgtt
     1801 gcgacacggg agcagcatcg caaaggcact gcaggaaaat catccgaggc gaccacgcca
     1861 attacagcga ctcaaaaaaa tcatcgcctg ttaggttaat gcttgtgggg cacacggcct
     1921 acaccatcgg acgatgcctc gtcttgtagg cccggtgccc ccaccgggcg ttcctggcgc
     1981 ggttccaatc caccaaccac cgccttcaat gccttgatcc attggccgag gcgcacggca
     2041 tcataaccca tgtgcgcaat caggtgtatg tggtcgggca tcacaacgaa acgccggccg
     2101 aaccaatggt aacgttgcgg gctgtcgagc aggaaagcaa cgagacgttg gaaggtgtcc
//
LOCUS       DASRKK010000413         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_813634, whole genome shotgun sequence.
ACCESSION   DASRKK010000413 DASRKK010000000
VERSION     DASRKK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813634"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFC15_09430"
     CDS             <1..533
                     /locus_tag="VFC15_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10417.1"
                     /translation="TGARIAEALNVRRSDIRLDSPAQVRVCGKGRKERISPLWPETVE
                     LLDAFLKRNPVAPDEVIFRNRYGNPLGASGVRFRLKRYLQAAAQRTPRLSEKRVSPHT
                     LRHTAAVHLLASGVDVTIIRDWMGHVSLDTTNLYAQANLETKRQALEKADGVLRPTPT
                     PRWKRDADLLAWLDSL"
     gene            1261..>2255
                     /locus_tag="VFC15_09435"
     CDS             1261..>2255
                     /locus_tag="VFC15_09435"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HZM10418.1"
                     /translation="MAGTNISGKKKNRWAAGVTPYAEMGYYNADYEPKDTDILCAFRL
                     VPHDGVEPIEAAAAVAGESSTATWTVVWTDRLTPYEHYQAKCYRVDPVPGTDQYIAYI
                     AYDIDLFEEGSIANLTSSIIGNVFAFKALKSLRLEDMRIPPHYVKTFQGPPHGIVMER
                     EYLNKFGRPLLGATIKPKLGLSARNYGRVVYEALRGGLDFSKDDENINSQPFMRWRDR
                     YLFCMEAVNRAVAVTGEIKGHYLNVTAGTMEEMYERSEFARELGSVIVMIDLTIGYTA
                     IQSMAKWARKNGVILHLHRAGHGTYTRQKTHGVNFRVIAKWMRLAGVDHIHAGTVV"
BASE COUNT          506 a          651 c          652 g          446 t
ORIGIN      
        1 acaccggtgc tcgtatcgcg gaggcactca acgttcgccg gagcgacatt cgcttggact
       61 ctcccgcgca ggtgcgtgtt tgtggaaaag gacgtaaaga gcgcatctcg cctttgtggc
      121 cggaaacggt tgagcttttg gacgcgtttt tgaagcgaaa ccccgttgcc cccgacgaag
      181 taatattccg caaccgctac ggcaaccccc tgggtgcttc cggtgtgcgc tttcggctca
      241 agcggtacct tcaggcggcc gcacaaagaa ctcctcgatt gagcgagaaa cgggtttctc
      301 cccataccct gcggcacacg gctgccgttc acctgcttgc ctcgggcgtt gatgtcacca
      361 tcatcaggga ctggatgggt cacgtcagct tggacacgac aaacctttac gcacaagcca
      421 acctggagac gaaacgacag gctctcgaaa aagctgatgg ggtgttacgg ccgacgccca
      481 caccccgttg gaaacgggac gctgatctgc tggcatggct cgactccctc tgagccagtt
      541 gcccatcgaa acggcgacat cgccccaacc tgccggtggc cggtgaggcg aaattatgga
      601 gagcaacagc gatcctgagc ccctgttaca agcatctctg ccggtctgct gctcataaat
      661 atatgctgcg cataactacg catcttcgta tgtgaaaatc ttgcgtttca cggggaatac
      721 acacccgcga caatgtgaca gaaacgggat cgcgcggagc ccttcgtgtt tcgaccagag
      781 actccacgta gcgcacatag aacgtcatag cacatcgtca atcgagtttt tggtaggggc
      841 agggattgtt gccactcttc aataatcggg ttgtggaaga gtcgctcgta atccaagcgc
      901 aatctcgaga gtcggcgtaa ctggccgtga atcagcctct ttgatgtgaa tccgtccgcc
      961 cagaaagtgg cttcgagata cgccgttccg tatgcgcgcg gttagcactc cccgatgagt
     1021 cacagatcac tgacaaatgt ctgccgcaag tggaagaatt acagcgatgt cgcggaagaa
     1081 attcccggct tcccagtgcg ggcgcggagg gctccgagcg aaccataagg gaatgcttga
     1141 cgttggcgac ggttcgagct tgccagggcg aacggcaggt tgaccgccag gattatcggc
     1201 ctgcgacgag gcgtcaaagt gcggacggca ggtgaagtcg gttgtttcaa ggagatcaaa
     1261 atggcaggta ctaatatatc gggcaaaaag aagaaccgct gggctgctgg ggtcacaccc
     1321 tatgccgaga tgggatatta caacgcggat tacgagccca aggacactga catcctgtgc
     1381 gcgttccggc tggtgccgca cgatggtgta gaacccatcg aagctgcggc tgcggtggcc
     1441 ggcgaatctt ccaccgccac ctggacggtg gtctggaccg atcgcctgac tccttacgag
     1501 cactaccagg cgaaatgcta tcgagtcgat cccgtacccg gcaccgacca atacattgct
     1561 tacatcgcct acgacatcga tctgttcgag gagggctcga ttgccaacct cacctcgtcg
     1621 atcatcggca atgtcttcgc cttcaaggcc ctcaaatcgt tgcgcctgga agacatgcga
     1681 attcctcccc actatgtcaa gaccttccag ggaccgcccc acggcatcgt aatggagcgc
     1741 gagtatctga acaagttcgg gcggccgctg cttggcgcca ccataaagcc gaagctcggt
     1801 ctatcggccc gcaactacgg ccgcgtagtg tatgaagccc tgcgcggcgg gttggacttc
     1861 agcaaggacg acgagaacat caatagccag cccttcatgc gctggcgcga tcgctatctg
     1921 ttctgcatgg aagcggtgaa ccgcgccgtg gctgtgactg gcgagatcaa ggggcactac
     1981 ctgaacgtca ccgccgggac catggaggag atgtacgaac gatccgaatt cgcccgcgag
     2041 ttgggcagcg tgatcgtaat gatcgacctg acgatcggct acaccgcaat ccagtcgatg
     2101 gccaagtggg ctcgcaagaa cggcgtcatc ctgcacctac accgggctgg gcacggcact
     2161 tatacacgac aaaagacgca tggagtcaac ttccgggtga tcgccaagtg gatgcgcctg
     2221 gctggagtcg accatattca tgccggcacg gtagt
//
LOCUS       DASRKK010000414         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_716386, whole genome shotgun sequence.
ACCESSION   DASRKK010000414 DASRKK010000000
VERSION     DASRKK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716386"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFC15_09440"
     CDS             complement(<1..876)
                     /locus_tag="VFC15_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HZM10419.1"
                     /translation="MHENRETSLPTARASGSPAGEGESRTSGMYGNEESDRAVVPMKP
                     SNQVTGQSPEAAEDPEGTPEGRARTEENVQKARAVPAQDGAAASQGLKGVRKAARERK
                     QEPFTTLLHHLTVDLLRDSYYALKRKAAPGVDGVRWAEYEDGLEPRLRDLHERVHRGS
                     YRAQPSRRIYLQKPDGRQRPIGIAALDDKIVQQAVVTILNAIYEEDFRGFSYGFRPGR
                     SPHNALDALSVGIERKGVNWIVDADIRGFFDSMSHEWTMRFIQHRVADKRIHRLIQKW
                     LKAGVSEDGEWSETTI"
     gene            1313..1645
                     /locus_tag="VFC15_09445"
     CDS             1313..1645
                     /locus_tag="VFC15_09445"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM10420.1"
                     /translation="MPGDHDLDLRGRLIRSLGALREMLPGSFVERHRKCGKPNCRCAD
                     GKQLHSEFQVSVLLEGKLKTFHIPAELAEEARSKVEMHKRFQEAAATIGHINLRRFLQ
                     RKGEKEKR"
     gene            1648..2976
                     /locus_tag="VFC15_09450"
     CDS             1648..2976
                     /locus_tag="VFC15_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10421.1"
                     /translation="MHRHQFEAYLDKVFDWRRLTAALTEGRQYPLYPWSEAFDAVFLG
                     SACQFGPLHRIETECRRGALHKRIGPLSEDMLAYAMQRQNPAEIFDLGCQVAQQLKRN
                     GVLQSDWARGRVVAAVDGIEICSSYCRCCDACMSREVERKVEGQLQKSTQYYHRLSAV
                     TVVSTPFPIFLGVRFQQNGETEVACSLALLKDLSEKLGRRFIDILVADAIYLQTPFVR
                     EVESLALDWVINLKDNQPDLLAEAGRLTAGPPQHHETTPQQDLQLWHVPEVDWPVADR
                     VVRVVKTVRVQNAKCVKVEERPVEVPQIEEPKAEKTEGRNSKGKKPKRSRVKTKRPAP
                     IVSTNFYASNVDLGAIPPYFIHQLGRSRWMIDAQAFQTITTDCHLKQPSAHQDCALIV
                     LTMIRVLAYTLTMVFYHRQVRSHCRGVPPGFCDMARQLAYCFLTPCSDTS"
     gene            complement(3279..>3483)
                     /locus_tag="VFC15_09455"
     CDS             complement(3279..>3483)
                     /locus_tag="VFC15_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10422.1"
                     /translation="HGALIVFDVGSVCHFVPTFEPVGGPFGDTDGGPCDSAGDEPIVK
                     PIGVRCGNQSSSGTTSAGYNQYG"
BASE COUNT          663 a         1136 c          923 g          761 t
ORIGIN      
        1 tatcgtcgtc tccgaccact cgccgtcctc gcttaccccc gctttcagcc atttctggat
       61 caggcgatga attcgcttgt cggcgactcg gtgttggatg aaacgcatcg tccattcgtg
      121 actcatgctg tcgaagaagc cccgaatgtc cgcatccacg atccagttca ctcccttccg
      181 ttcgatcccc acgctcaacg catccaatgc gttgtggggg ctccgtccgg gccgaaatcc
      241 gtaactgaaa ccccggaaat cctcctcgta tatggcgttg aggatcgtca ccactgcctg
      301 ctgtacgatt ttgtcatcca gcgccgcgat gccgatcggc ctctgccgac cgtccggttt
      361 ctgcagataa atccgtcgtg acggctgtgc ccggtagctt ccccggtgca cccgttcgtg
      421 cagatctctc agccgtggtt ccagtccgtc ttcgtattcc gcccacctca ccccgtccac
      481 tccaggcgcg gccttacgtt tcagcgcgta atagctgtcc cgcagcagat ccacggtcag
      541 atggtgcagc aacgtagtga acggttcctg cttcctttcc ctggcggctt tccgcacgcc
      601 cttcagaccc tgtgacgcgg ctgctccgtc ctgtgccgga accgcgcgtg ccttctggac
      661 gttctcctcg gttcgcgccc ttccctccgg ggtaccctct gggtcctccg ctgcctcggg
      721 tgattggccc gttacttggt tcgacggctt catcggtact acagcgcgat ccgactcctc
      781 attgccgtac atgccggacg tgcggctttc gccttctccg gccggactgc cgctggcgcg
      841 cgcagtcggt aatgaggtct cccggttctc gtgcatacaa tttcgcggcg tgccagggtt
      901 ctcagactac gtgggtccgc cgcccggctc gcgatctagc gccgcgacag cgtgttgcct
      961 tccccttcag acgacagggc cggcgaccgc agttcgtggt tttcgaagct ccataccctg
     1021 cccgtcgctg cccctgtcta cgcttcggac cgcgcctcat gatgcgcccc gcaagactcg
     1081 gggtcaggat ggattcgctg ctcctttcct gtaggactct ttcattcccg attgcacgcc
     1141 ggtttatccc ggcgcaccgc ctgtcccagc gatttcgcaa cgtgcctgag cttgccccac
     1201 taatccgctg aaactgcggc gagttcccag tctgatctgc cccaagtcat ttttgcaaat
     1261 gttgcaatac ccttgacttc cgctttgtct accattagta ttactggtag atatgccagg
     1321 cgaccacgac ctcgatctgc gcggcaggct catccgcagt ctgggagcgt tacgggagat
     1381 gctccccggc tcctttgtcg aacgccaccg caaatgcgga aaacccaatt gtcgttgcgc
     1441 tgatggcaaa caactgcact ccgagtttca ggtttcggtg ctgctggaag gcaagttgaa
     1501 gaccttccac atccctgccg agttggccga agaggcccgc agcaaggtgg agatgcacaa
     1561 gcgttttcag gaagcggcgg ccaccatcgg ccacatcaac ctgcgccgct ttctacaacg
     1621 taaaggggag aaagagaagc gctgactttg catcggcacc agtttgaagc ctatctcgac
     1681 aaggtgttcg attggaggcg cttgacggca gcgctgaccg aaggtagaca gtatccactt
     1741 tatccctggt cagaagcttt tgacgccgtc ttccttggat cagcttgcca gtttggtccg
     1801 cttcaccgta ttgagacgga gtgccgccgc ggcgcactgc acaaacggat tggacccctc
     1861 agcgaggaca tgctcgctta cgcgatgcag cgccaaaatc ccgccgagat cttcgatctg
     1921 ggttgccagg tggcccagca actgaagaga aatggcgtcc tgcaatccga ctgggcccgc
     1981 gggcgagtgg tcgccgccgt cgatggaatc gaaatctgca gctcctattg tcgctgctgt
     2041 gacgcctgca tgagcaggga agtggaacgg aaagtcgagg gccagcttca gaagagcacg
     2101 caatactacc atcgcctctc ggccgtcaca gtggtcagca cgccgttccc tatcttcttg
     2161 ggcgtccggt ttcagcagaa cggtgagacg gaggtggctt gctcgctggc gctgctgaag
     2221 gatctctccg aaaagttggg gcgccggttc atcgatattc tcgtcgccga tgccatctat
     2281 ctccagactc ccttcgttcg cgaggtggaa tcactcgccc tggactgggt catcaacctc
     2341 aaggacaacc aacccgacct gctggcggaa gccggacggc tcaccgcggg tccgccgcag
     2401 caccacgaaa cgactcccca acaagaccta caactctggc acgtgccgga ggtggactgg
     2461 cccgtggccg accgtgtcgt gcgagtcgtg aagacggttc gggtgcaaaa cgccaagtgc
     2521 gtcaaggtcg aggaacgccc agtcgaggtg ccccagatcg aagagccgaa agcggaaaag
     2581 actgagggca gaaattccaa gggcaagaag cccaagcgct ctcgggtaaa aaccaaacga
     2641 cccgctccca tagtcagcac caacttttat gcgtctaacg tggatcttgg cgccattccg
     2701 ccgtacttca tccaccaact cggacgcagt cgctggatga tcgacgccca agcctttcag
     2761 accatcacca cggattgtca cctgaagcaa ccctccgccc atcaagactg tgctctcatc
     2821 gtgttgacca tgatccgtgt ccttgcctac acactgacga tggtcttcta ccaccgtcag
     2881 gtccgcagcc attgccgggg cgttccacca ggtttttgcg acatggcgcg gcaacttgcc
     2941 tactgctttc tcaccccctg ctccgatacc agctgatatc cgctggctaa ccccaggccg
     3001 tcgtcttctc catcctctcc cgatgggctg gtcctctcgc gggaaccccg aggtcctcct
     3061 ttttctccat ttcaaaaatg atccggtgat gctcgcctgt cagggattct cgtgcccaaa
     3121 gtgcctaaaa aagcaagcgc aaaaatgcgc tccttggcgt ttcacgcaaa agtcgcgctc
     3181 ccacagcgac ttaacccgcc tccgcacacc acgttgcgaa atcgctggac cttccgttgt
     3241 atcccttgca ccaagaagag tttgcggcta cgctggtttt atccatactg gttataacca
     3301 gcggatgttg ttccgctgga actctggttt ccgcagcgaa ccccgattgg cttcactatc
     3361 ggctcgtcgc cagcggaatc gcatggaccg ccatctgtat caccaaacgg gccgccaaca
     3421 ggctcaaagg taggcacgaa atggcacacc gaacccacgt cgaagacgat aagggctcca
     3481 tga
//
LOCUS       DASRKK010000415        42762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_911549, whole genome shotgun sequence.
ACCESSION   DASRKK010000415 DASRKK010000000
VERSION     DASRKK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 42762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42762
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911549"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1698
                     /locus_tag="VFC15_09460"
     CDS             151..1698
                     /locus_tag="VFC15_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10423.1"
                     /translation="MDATSDPGSIVRFGVFEVDLRAGELRKNGGKVKVQDLPFRALKL
                     FLSRPNQVLSREEFRQALWPDGVFVDFDRGISSAINRLRESLSDTASNPRFIETVARS
                     GYRWIAPIQVVSASDERVADQGIGARRPAQVEQVAVAPAARNFSLKKIWVLAAGVAVL
                     AMAVVLVMKWRPSSVTSARNNSGVTAVTTVSAPSPEVEELYLKGRYYWNKRTPEALNH
                     AVDYFSQAILRDPNYAKAYVGLADSYNLLREFASMPESEAYARAFVAASKAAELDPSS
                     AEAHATLGFLYFWSKRDLAASSREFERAIALNPNYVDAYHWYGNVLASAGRSEESLTY
                     LNRAQELDPASPSIRADKGIGLVAAGHRDDGVALLKQMEATDPNFISPHSYLAAIYLG
                     EMDCSGYLTEARAHARLQRDPDKLAMVQAAEKGFAAGGCPVMLRSILEVQKKRYAQHR
                     VTAYELAETEALLGDKPATLQYLHLSLAQNEAALANLRACSYFRGLYTEPEFRQLVLD
                     TGLPPLT"
     gene            complement(1749..2189)
                     /locus_tag="VFC15_09465"
     CDS             complement(1749..2189)
                     /locus_tag="VFC15_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10424.1"
                     /translation="MTKTAIPESPAKPVNPVQLDHILTSAIFLNWEALTASPTGGKVR
                     IEYHIGPGGSVEYLTIWGSAREYWSLICNYSVNPGWSDGPSFSNGYHSRTLGRLLQSI
                     MMNQNLFFHGCKPNTNGMLQIGAPSLQDTDNARLRVNETFQRAS"
     gene            complement(2296..2838)
                     /locus_tag="VFC15_09470"
     CDS             complement(2296..2838)
                     /locus_tag="VFC15_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM10425.1"
                     /translation="MNQGRQQPVTKTLAPKLSILLIDANSERRALRKKIMATHGVEVI
                     GASDVTEAGAIWQRDRYDMVLIDIRADHRGCLAFRDEIKKDSAGQIVAFLVGRPKFID
                     LEPLLGSYIAEAHGSQWGDSMRKAVRESCDSLLQRNGLVEATWRIAAGRKMNGTPHNV
                     LESSVPAISPLDFIADSLDA"
     gene            3203..5572
                     /locus_tag="VFC15_09475"
     CDS             3203..5572
                     /locus_tag="VFC15_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10426.1"
                     /translation="MRAKNNPKKPVPMLAITVAVLVAAVCAVVAWRWSTRVHGQETKK
                     ASSSTSAAYVGQERCARCHAEQEQAWRTSHHAQAMQVANDSTVLGNFNDARFAKDGVT
                     SSFLKKDGKFYVRTDGPDGKPQDYDLPYTFGVSPLQQYLVPFPGGRMQSFVVAWDSRS
                     KDHDGQRWFHLYPDQKIGSTDPLHWTGRNQTWNYMCADCHSTNLRKNYDLAKDSYDTK
                     WSEIDISCESCHGPGSNHVAWAQTHKKGSYNPGEGSNGLVVDLRPASGSWASLEPDTG
                     TMHWKGQARSRNEIDTCAPCHARRHPITSDNEPGQPFLDGYVPSLLEEGVYYPDGQIL
                     EEDYEWGSFIQSKMYHEAVTCSDCHDAHTAKLAPVSLNAVCGKCHSLQKFGGEQHHHH
                     KAESAGALCVSCHMATKTYMVVDVRRDHSFRVPRPDFSVAYGTPNACTQCHKDKSNSW
                     AVDTVVKWYGPNRRQEAQFVTAIEAGRRGLPNAAKALTALITDTAKPGIARATALSLL
                     PAYLTPSALPAVQSALVDNDALVRSQAVRSLEPLSPKERVQLAAPLLTDPVRYVRIEA
                     ARLLAGTPAEQLQDAQKTALDHAIAELIASEMASAERPESHMNLALLYAQMGRPNEAE
                     SELQTALRLDPKFVPAMVNLADLYRTQQREQEGQQWLERAIAVEPNAAEPVYALGLLK
                     IRQKNYQDALMLLAKAASLQPNNTQYSYVYAVALNSAGHADGAISVLEQAHQKRPADV
                     QVLDGLIAFERDKGNLPAAITYAQQLVQLTPDDPSAKAMLAQLQQQVRQ"
     gene            complement(5597..5749)
                     /locus_tag="VFC15_09480"
     CDS             complement(5597..5749)
                     /locus_tag="VFC15_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10427.1"
                     /translation="MQTKVKQQSIAIERLNGALQEKEAALASMYKSKSWRITSWFRKI
                     AAVGKK"
     gene            complement(5942..7099)
                     /locus_tag="VFC15_09485"
     CDS             complement(5942..7099)
                     /locus_tag="VFC15_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10428.1"
                     /translation="MVRIRIPNGLLLSHQLRTIADVTEKYARGSADITVRQNIQLHWV
                     TIGDLPNVLDALWRAGLTTMGSCGDDVRNIIGCPVAGVDANELYDASSLVNQANRQFV
                     GNSEFYNLPRKFKICITGCHAWCSYPEINDVGLTAVRRNGREVGFSLRVAGGLSTDPH
                     LAKRVDAFVLPNQVLPVLKGIAPIFRDSEVLRENRDRARLKFLFLKHGWTADHFLSEL
                     KGCLGFELPPAVPEEIPDDIYRDHVGIRRQKQHGYCYVGASVLRGRISAAQLHAAADL
                     SERYAAGELRTTNMQNLLIVNVPERNAPILAGALQLVGLPTEGSPFRRGIIACSGTEF
                     CKLAITETKSFGRWLAEELDERLPGFEQHLQDGSRWIALWTRRPGDDSNRP"
     gene            7850..9826
                     /locus_tag="VFC15_09490"
     CDS             7850..9826
                     /locus_tag="VFC15_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZM10429.1"
                     /translation="MTIPSRAIRYCLLFLATFLASSLLLNAGTDWAPVDPAELQMKDL
                     PEQPGAPAFVLFHEEIDNDQQGSHSVYMRIKVLSEAGRKYADVQIPYYREAFTISDVH
                     GRTIHPDGSIVEFQGKPFDKVVVKSKTLKVREKAFTLPDVQTGSILEFKYILRSENGL
                     LLPPRWTVQNDLFQRKEHFKFVPYAGRIQMAHGEVGTGLAYTWMLPKGAEVKNTRDNY
                     ELDLANMPAFIEEEHMPPPQQYKYYVRFYYKRLSNTDQYWKEEGKYWNSDVEKFLGKK
                     HGIPEAVAGIVSASDTPEQKVKKIYAYVAALDNLSFKPHRTEQEQRVLGVKDNRGVED
                     VLRQRSGDRQEMTLLFIAMVRAAGVPAYAMYVSDRSEDVFEKTYLSFDQLNAFVAIVQ
                     IDGKDVFLDPGTKFCPYGLVDWKYTGTRGIKQLPGGGTEIAETPPPDYMKAIIKRVAR
                     LQLNEQGQVEGSLAIGFFGQEALTRRVEASKTDEVGRNKILEDEVKSWLPSNAQVTLT
                     KPPEWEAVETPLVAEFKISSPMLMSGGKRVLLPANVFEFNRPPMFAHAEHTHPIYFEY
                     PSREVDDVHIKLPVNLQVESLPANQDVKMEYALYRAGRKQEKNEIDMTRDLAIGAFVF
                     SPTEYKNLKSFFDKVKEGDDEQVLLKQVAHVAQN"
     gene            9810..11768
                     /locus_tag="VFC15_09495"
     CDS             9810..11768
                     /locus_tag="VFC15_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZM10430.1"
                     /translation="MSRKTRFAVTVLVALACSAPLLGSSPSAPDWLHQAAAKAPGKYA
                     PETNAVVLLDDTTLTVTGPGQADETRRRIVLILRPQGREEAKLHVFVGPGDKVQAIHA
                     WTIDSAGVQYEVKDKEFIEVSPYEGALYTDIRFRTAEAPAGNPGSLIGWEYTTRRHIW
                     MDQWHWFVQENIPVEETRLTLQLPASWEYKASWANQAAQQPAQTGPNRWQWICLNLPG
                     IPEEHLRPASEALEGHLELAFYEPATASNLGSWKAIGDWYYKLVDGRRNPSPEMSARV
                     QQLTAGATTFDGKVRALTAFLQSDVRYVEISIGIGGYQPHPAADVYRSRYGDCKDKVT
                     LLSSMLKEAGIDSEYVLVDSERGVVKPDVPSTLFNHAILAIDLPKDVPPNAYRSVSTT
                     TSGTRYLIFDPTDEYTPVGDLRTALQGSYGLLVTKSGGELIKLPVLLPDTNRFDREGK
                     FTLQADGSLSGTVTERFTGTHAARQRAWLGTQNESERAKSLDHRLGESLKNVSVQQLK
                     VQDLAAHDKELTLTYQLTAQSYAQKTGPLVLVRGRVLGEKAVEINWAKRKFPIQLSGA
                     TSEKDSFEIQIPPGFAVDDLPEPKQIDVGFASYKSKVEANGSTLHYTREYVVRDPYIG
                     SDHFSDLRKLEGTIGDDEFASAVLKKTP"
     gene            complement(11819..12226)
                     /locus_tag="VFC15_09500"
     CDS             complement(11819..12226)
                     /locus_tag="VFC15_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10431.1"
                     /translation="MRRSSSYWKIVFAVIATCLTGSVAMYSQQPSANPPKPPAKANPS
                     QVQSIVLPQYPPQIANGPNLETYRKNCLLCHTARYVSTQPRFSKSVWQNEVKKMVDAY
                     GAPISEADQALIVEYLVAVRGAEVPVTPLAPPK"
     gene            complement(12223..13473)
                     /locus_tag="VFC15_09505"
     CDS             complement(12223..13473)
                     /locus_tag="VFC15_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM10432.1"
                     /translation="MGTMLTTHSSRIDRRRFVSLLAWLGGFAAGRRALGFPAEASSGP
                     GFETLPTEPAARQLVRYPQKTDLILLTDRPPQLETPLRHFHNDLTPNDAFFVRWHLAG
                     IPTTVDLRSFRLEVGGHVSKPLNLSITDLKSKFEPVSLVALCQCAGNSRSLFEPRVPG
                     GQWANGAMGNARWKGVRLKDVLGAAGVLPGTIQVGLHGLDVPLMPKTPRFEKSLPIDR
                     ARNSDVLIAYEMNGEPLPMLNGFPLRLVVPGWYATYWVKSLSSITALEQPLKTFWMDK
                     AYRIPDNSNANEDPLRLSPATIPINRMSVHSIFVRPAPDEQLHAGISYELAGVANDGG
                     DGIRRVEISFDGGQTWSDAILGIDLGKYSWRRWQARWTPPAKGSYRLLVRATNSAGQT
                     QPAAQWNRSGYQRGVIEHVDVIVV"
     gene            complement(13627..14337)
                     /locus_tag="VFC15_09510"
     CDS             complement(13627..14337)
                     /locus_tag="VFC15_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM10433.1"
                     /translation="MQSPYGMELIENCLSCNLCSEGFFCRLPKPEMEAFQKIKFTLAY
                     PAGATLFVEGQACRGIYILCRGRVKLSATSSQGQTLIFKIAKPGEVLGLNATVSGSPH
                     DTTAETGQACQLNFVKQADFLRFLTENRGASMHAAMHLSRECQQAYQQLRSFVMRSAP
                     ERIARLMLEWSLAGAGMSTSHGIKVALTHDEIGQIIGMSRETVTRTLADFRKHHIAVL
                     HGSTLLIENMSALEHLAC"
     gene            complement(14728..15438)
                     /locus_tag="VFC15_09515"
     CDS             complement(14728..15438)
                     /locus_tag="VFC15_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM10434.1"
                     /translation="MQSPYGMELVENCLSCNLCSEGFFCRLPKEEMEAFQKIKFTLAY
                     PAGATLFVEGQTCRGVYILCRGRVKLSATSSQGQTLIFKIAKPGEVLGLTATVSGTPH
                     NTTAETGQACQLNFVKQADFLRFLTEHSVACMHAAIHLSRGCQQAYQQLRSFVMRSAP
                     ERIARLMLEWSIGGEGTAATLGIKVALTHDEIGQIIGMSRETVTRTLADFRKHHIAVL
                     HGSTLLIENMPALQNLAT"
     gene            complement(15730..16386)
                     /locus_tag="VFC15_09520"
     CDS             complement(15730..16386)
                     /locus_tag="VFC15_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM10435.1"
                     /translation="MRAKNRRKFEPRAFLATIGTGRKILAFEKGQNIFAQGGAADAVF
                     YVQKGKIKLTVVSQAGKEATVGILGEGSFFGEGALAGQAFRMGSASAMTECTILRIDK
                     KSMMQALHREQSFSDLFVSHLLARNIRYEEDLVDQLFNSSEKRLARILLLLAHFGKEG
                     KPEIVIPKISQESLAEMVGTTRSRVSFFMNRFRKLGFVEYGGGGLQVHSSLLNVVLHD
                     "
     gene            17591..17812
                     /locus_tag="VFC15_09525"
     CDS             17591..17812
                     /locus_tag="VFC15_09525"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZM10436.1"
                     /translation="MKWEQVENKWEQLIGSAKENWGRIGDDDFRLISGKREQLALKIQ
                     EAYGITRREAEKQVWDWGKTVENVAKKIA"
     gene            18672..20483
                     /locus_tag="VFC15_09530"
     CDS             18672..20483
                     /locus_tag="VFC15_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM10437.1"
                     /translation="MAENTPVEMKATLGLTGLTMNAMALIAPGAFLWLTFFIQATTGV
                     TAPAMWLGIVLALALCLATAVCYAELAKLYPGTGSSYYFAEQSFLNHDKAWRYARLSK
                     FIVGWASHLYYWIYPGVMVAVMGILCGYLVGTIWPSFMSASNPGVAFMMVVAIVFSFA
                     VAYIAHRGVTGSTAVSIAINVIQIAALVLFAVLALGYRMNHPPGTVAYQFDSVSGEAY
                     TYEFATTTTLVDGKPTDTIVRDSAGVVPQPKLDTAGNPVPFKISYPETDSSGAFLSHP
                     NAKSVIRAHNWGWVFIQATVAILILVGFESVTSMGGEARNAKRDIPIAVIVSLLVQGL
                     FCYLFEYFAANYFLNSGYSMANASGSAAPIGDMMVVVGDALLGPGHGRTFMLCEAFTV
                     FLALIGTTLSCMNTGARVTYAMGKDKEAPEHFGMLHSENLSPHRAIWTLAVISAVVAC
                     LSLIFVFGDAGAPTDAAIQALPHGFWSSFGYISHDREAAMPNTLLTMTLASNFGTFLL
                     YGLSCVICLVGYHNHPKFKLVRHLVVPVFGLVANLACMAFYLIGPFMGYGTKKEPLLA
                     LGVALVWFIYGGIYFIRSSKSTGRNTLIERGVSASVV"
     gene            20630..21427
                     /locus_tag="VFC15_09535"
     CDS             20630..21427
                     /locus_tag="VFC15_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZM10438.1"
                     /translation="MLDVEFTQITDPGRVREHNEDYLGYVLPHVAAQLRSHGCLFALA
                     DGVGGQQKGEVASRLAVESVLDGFVRAREGEPHKALLTRLVQAANTQVYEMGGSSGSR
                     TGMATTLVACALRFDHAVVAHVGDSRCYLIRSGKPKLITNDHTVVNEQARLGILSARE
                     AAESTNKHLLSRSVGNDMFVAVEINDVQVEKGDVLVLCSDGLHGPVPEPDIASIVTEN
                     QYLSTAAERLIALANQRDGSDNVSVQLIRVHDVERTGMYRGRPYKIR"
     gene            21631..22599
                     /locus_tag="VFC15_09540"
     CDS             21631..22599
                     /locus_tag="VFC15_09540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM10439.1"
                     /translation="MTPVRTDEQLDHYRIDQLIARSGMASIYRATDLRNERQVAIKIP
                     HPEMESDPLLYDRFRREEEIGKNMDHPGVMKVFTDDHRSQVYMVMEWVDGRLLREILN
                     EQKKLPQERAIKITTRILDALQYIHDHGIVHRDLKPENVMVDADDNIKLIDFGIASDL
                     HGRRLTFANLSQTMGTPDYISPEQVKGKRGDARSDLYAVGVMLYEMLAGKVPFTGTNP
                     FLIMNDRLLNNPVPPRELDPAISPQLQEVIYRALERDWKNRYARASEFAWDLEHLDQV
                     GVAERAEMRDWKTRRSPWPRKIAFYVAMALIPVVVFGLLLWVARHS"
     gene            complement(22645..23961)
                     /locus_tag="VFC15_09545"
     CDS             complement(22645..23961)
                     /locus_tag="VFC15_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10440.1"
                     /translation="MIPASAPAFSDTSAGAVCLVSILLIPLAMAGLAVMNSGLGRSRS
                     AAHVMLASLCAISVAAIVYVVCGFSWDGFAGGPAHVWLLRGKPWNWIAAEPFFFRGIE
                     LDGSPASLAALLQLFGVALAALIPLGAAMDRWRLGAICASTAILAGCTYPIFAHWVWG
                     GGWLAQLGVNYGLGQGFLDCGGAATIQVVGGLTALSMVWILGPRFSKYTPEGMPTGIP
                     GHSLVFVVFGCFLTWLGWLGLNSAGALLFNHIPAGRVVLIAVNTTLSAAAAGLMAVTI
                     TRIRFGRPDASISANGWVGGLVASSAASPFLKPAAALLIGFIAGVLVTFAVEWFEMWL
                     KVDDPGGAIPVHAVAGLWGLLAAGLFARFPGRNSADGQWLAQVIGIATLLGFVLPLTY
                     SLNWLLNRFYRQRVDPEGERQGMDLYELGTGAYPEFVFHPDEFIQR"
     gene            24351..24713
                     /locus_tag="VFC15_09550"
     CDS             24351..24713
                     /locus_tag="VFC15_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10441.1"
                     /translation="MKDILNQIVATRTGTFVVLLLAAFLEAYGDSCFQTGLYRSSGAA
                     RYAAFAGGAITLSLYGLVVNTPPWEFGKLLGLYVVLFFIVAQVLAKVRFNQAPTPPIL
                     VGGSLILLGGMIISFWRK"
     gene            complement(24728..27811)
                     /locus_tag="VFC15_09555"
     CDS             complement(24728..27811)
                     /locus_tag="VFC15_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZM10442.1"
                     /translation="MIHRIVQASLRQRFLVLMLTGFIMIAGIISFRRMPVDAYPDLSP
                     PQVELITQWPGHAAEEIERLVTLPIELEMNGVPHMVVMRSISLYGLSDVILTFEDNTN
                     DYFARQIVFERIAQAALPAGVTPNMAPLFSPSGLVYRYVLESPDRSPQELKTIEDWVV
                     ERAYRSVRGVADDSGFGGTTMQYQVLLDPARIYGYHLTVPQVMTALGANNSNAGGGFY
                     SQGGQFYYVRGIGLVRNTSDIGNIVVGSSNGVPIRIKDIGDVVIGYAPRLGQFGYQKN
                     DDAVEGVILMLRGEQTQNVLKGVEAKTEELNRNVLPPDVKIHPYYDRSELVKLTTDTV
                     EGNLLRGMILVLVVLIFFLVSFRAAIIVALTIPLSLLFAFIALHAHDGAANLLSIGAI
                     DFGIIIDGTVVMVENIYRELALREGQTYKLNEVILAAAKDVDRPIFYSVAVILAGYLP
                     IYALTGPSAKLFHPMAETMSFALVGALILTLTLVPVLASYWFKNGVREKSNRAYEWMK
                     DRYATQLDWALARPKLTMLIAVLIFGATLLLVPFIGGEFMPHLDEGALWVRATMPYTI
                     SFEEASRIAPQIRAILTSYPQVTVVASELGRPDDGTDPTGFFNCEFYVGLKPYKDAAW
                     SGDLNTKAKLIEAINQKLSAFPGIVFNYTQPAEDAVDEALTGLKSSLAVKIYGEDLGV
                     LQDKAIQIKNTLAKIPGFTDLTVVRELGQPSLLIDVDRDKIARYGINVADVEAVISAA
                     VGGQAVTQVIQGEKLFDLVVRMQPQFRSSAHDIGELLVGTPDGKQIPLTQLAEIKVGN
                     GASFIYRENNSRYIGVQYSIEGRDLERAVHEGQKAVDQAVALPQGYTLNWGGEYSQFL
                     EAKAQLVLIGPLAVVLIFMILFALYGNFKFPVTIALGVILTEPVGALIALKLTHTPFS
                     VSSVLGLLALLGVSVETAVILVSYINKLRLEGMDIRTATREASLLRLRPIMMTALVAC
                     LGLLPAALATGIGSDTQKPFAIVIVAGLISRLFIGFFVNPVLYQMVAREGDVLQV"
     gene            complement(27871..29037)
                     /locus_tag="VFC15_09560"
     CDS             complement(27871..29037)
                     /locus_tag="VFC15_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM10443.1"
                     /translation="MTIYNHPMGRRVALTVLFAIVLAFAGCSSAGPDNAAKNHANNSN
                     NAELFTIPSEQMAHVQVLTVQPANLTRSLRLSGAVAYNSFHTTPVITQVSGPVSRIVV
                     VPGQHVQRGEPLLYVASPDYSQLRTNYLKAKDAYSLAQKAYARAQDLYQHHALAEQNL
                     EQAESAQTQAQGDLTSAEAALKVLGITNPETLVKAPPSFEALVKAPISGEVVEQDVAA
                     GQLLQTGATQTFVISDTSTVWVLVNIYQKDLPYVHVGDQVTIQTESYPDVFRGRISYV
                     AASLDPNTRTLQARIETSNPGLKLKKDMYVVATVDAGTIQNAIAVPDSAVLRDSENQP
                     FVYVESAGNQFGRRTVTIGDSIKGQTQILTGLNPGNRVIGDGSLFLQFANSLQR"
     gene            complement(29034..30269)
                     /locus_tag="VFC15_09565"
     CDS             complement(29034..30269)
                     /locus_tag="VFC15_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM10444.1"
                     /translation="MTCLLLLVPALLGQNPTLITLDQAINLALAHNHSLKATRTLILQ
                     NQAQEITANLRPNPTLNFDSQFLPIFQPSDFSADALSEIQQFDVGIGYLFERGRKRQH
                     RLQAARDQTAVQIAQVSDAERTLTFNVAQQFVGVLLAESTLRFALLDLKSFEDTVDIA
                     QTQYKSGYISEGDYLKIKLQLLQFQTDVSSARLARVQALVALRELLGYDAVPSNYDVV
                     GDLSYEPVQAKLEDLQAEALQQRPDFRAAELGVTAAQSQIQLAKANAKQDVSANVDYT
                     HTADQNSASFFVNFGLPIFNRNQGEIARTGYALTQAQEMQSSASDTVLSDVSDAYESL
                     RSSEEVVQLYTSGYLKQAQDSRDISEYAYKRGATGLLDFLDAERSNRAIQLAYRQALS
                     TYMTALEQLKEAVGTRNLP"
     gene            complement(30536..32647)
                     /locus_tag="VFC15_09570"
     CDS             complement(30536..32647)
                     /locus_tag="VFC15_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM10445.1"
                     /translation="MLRPFIELRRKLTALVAMVGLLLPTALLAQQSQTGYTFKAQTEL
                     VLVNVTVRDKNGNPVRDLKPEDFIVLEDNKPQKVATFDIENTQNVQQVPMQQANLLST
                     PVTKTKTSATAPAASAQESAIKDRRLIILFFDLSAMQPDEIERSSNAAQDYLAKQMQP
                     ADLVSVVSLGNTITVNQDFTSDREKLKAVLQSFNVGANAGFELGSTGTTEGTPDSGQS
                     FTVDDTEYNIFNTDRRLQALRAIADQVANVPQKKSLIYFSSGMQRTGIENQSELRAAI
                     NSAVLANLSIYTLDIRGLQALPPGGEAQNASLRGTSAYSGQAQLNALNSNFQTQETLV
                     TLAADTGGKAFLDSNDFGRVFKGVQDDTSMYYLLGYHSTNTARDGKYRHITVRVNRPG
                     VKVDARRGYYAEADFRHSNKDDRERQLREELASELPSTDLPVYLATAYFRVSDNRFYV
                     PLSLIVPGSEIPFSRSRDQDKATLDILALVTDEKKFPVSEIRETVKLVVNASNEVQRK
                     NVQYDTGLTLIPGKYHLKVVVRENEAGRMGSFETDFTIPDLKSSPLKMSSIVMASQLQ
                     TAPKRDTGNPLVQNGQEIVPSVTRVFSANQHLYIYYEVYDPAKETAPEISPAPTDKNS
                     KPGVRVMSNVAFFQGDTKAYETPLVRVHQLTVPNRKAAVFQLDVPLSELKPGFYTCQV
                     NVIDDAAGRFVFPRLAMLVRQ"
     gene            complement(32656..36072)
                     /locus_tag="VFC15_09575"
     CDS             complement(32656..36072)
                     /locus_tag="VFC15_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM10446.1"
                     /translation="MASQVRRQWRMIGLLFSVLCCVSLVAQNPATPAPPPSDSSTNTA
                     QTGSANPEAITKTSQSPAPSAPPAPAGTLTGRVKSGNTLVPGVTVSATNSATGQKTVG
                     WTQADGSYTLALTSPGEYAVRVQMAGFAVATERVNVSTTNLHPRLDLQITLLSRAQNA
                     GPSAYARGGGAGNRGFQTLSVMAAEAGNNSNSSGSSDSVATAGMPVPGIPPSMATESV
                     AVSGSSASGNIFDMNSDEMRTRAQEFRNQQGGPGGPGGGFGPGGGPGGGPGGGGFGPL
                     GGGGMGGRRGGFNFNQPHGTLYYSANTSTLDATPYALSGQPSEQPGYLQQHFGASVGG
                     PLNIPHIYHGGSKTFYFFNYNGTYGDTPYNFLTTVPTALERNGDFSQTLLNGKPVQIF
                     DPATGLPFTNATVPQNMISSASLGLLKYIPTPNLPGTTQNFQYLTAATNNTTDLNFRL
                     NQAFGGSSVSPGRGRARGPQNNLSIGFHYHNVDQTLINSFPTVGGHTTTNGYDIPIGY
                     VRSFGKLVNNFRFDFNRSTIYTNNLYAFNTDITGQLGITGVSQNPFAWGLPNLSFTHF
                     GGITDTNPVNDRNQTWTFTDNMIWSHGKHTVRWGGDFRRIQLNTQTDSNPRGSFVFTG
                     ALTSQFVNGVAVPGTGYDFADFLLGTAQQTSVQYGYDRYYFRGNSWDMFVQDEWKARG
                     NLTFNVGLRYEYVSPLSESNDRIVNLNTNSSFTEVAPVLPGQVGPVTGSVYPITLVNP
                     DRNNFAPRVGVAWKAAKNTVVRAGYGINYNTAAYQNIVQNMAFQPPFSNTQTNVASPS
                     NILTLQNGFPTPTSLTNNYGIQQNYGLGYVQIWNLDIQQEITRTLLLNLDYTGTKGTR
                     LDILSAPNSTATGVLVPGVQPYYYESSNGDSTANAGSVRVRKHLSQGVSLGGTFTWSK
                     SLDDASTIGAGSSIVSANGKITGVTVVAQNAFDLSAERGLSSFNQEFRFTSDYLWELP
                     LGKGRRWLSTAGLAQDLLGGWQWSGDWTIASGLPFTPRILGSYSNVNSGVNGTLRADV
                     TGLPVSVPNPSIGEWFNTAAFVIPPTGQYGDARRNSIIGPGQVLFDMAMTKLIPLKES
                     RSLELRLSSTNVFNHPIYSSIDTVLNSPTFGRVISVSAMRAVLLTARFRF"
     gene            complement(36397..37554)
                     /locus_tag="VFC15_09580"
     CDS             complement(36397..37554)
                     /locus_tag="VFC15_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZM10447.1"
                     /translation="MRLVLVFGTVLVTVVAASSQSNMVDQMTTPERLRQQVWWPTKLS
                     SAPDKFVGNEVCAACHAAIVKSQATSEMAQTLMPAEQSPFLTSRYGKQVTVDGFVYRI
                     FRGPEGPAFQVVSSTSPITKPLTWAFGSGKISQVYSTPEEDHFNESHFSYFESIDGFD
                     LTPAQPSVRVASTIGEQHSDAMKRAGGRTVNMVEARRCFSCHAADVPASGPIANVIPG
                     VSCEACHGPGKDHTVAMQAGLPQGTALIMNPARLRPVDQVDFCGACHATSMDIQLDGG
                     LGLPTVRFPAYRLQNSRCWRNDSRIQCTACHDPHQPIVHEVSSYDQKCLACHVTAAQS
                     KSAADRPGHACPVNAKDCAGCHMPKYDFPDVHHKFTDHDIRVVRAGAPFPG"
     gene            complement(37612..38709)
                     /locus_tag="VFC15_09585"
     CDS             complement(37612..38709)
                     /locus_tag="VFC15_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM10448.1"
                     /translation="MFRRRVLMVAGCVAFAPLPCAWAQSQTVISPKPAEQVYKNIQVL
                     KGTPADQLIPAMQFITQSLGVRCEHCHVEHAFDSDDKKPKQTARKMIQMVLALNQNNF
                     AGERDVTCYSCHRGNLRPANTPAVASETTAPKRTPAAISASRSVDQVLTKYLQAIGGD
                     SALQAAKTQGEKGVVNLGNGVQFPVEIYTKSPGMRCMTMHLPTGDSLEVVSGPSGWIS
                     VPGRPVHEMNSSDLLAAHLDADLQFAADIKSLFPTIESRPDTEIDGHKVNVIFAISPG
                     LPPVELYFDAESGLLVRMVRYVESPLGRTPTQIDFSDYRVVAGTKVPYQRTISQPQGR
                     FLVQLQEIQRNLPIEDSRFAEPVTGATTAAQ"
     gene            39151..39603
                     /locus_tag="VFC15_09590"
     CDS             39151..39603
                     /locus_tag="VFC15_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10449.1"
                     /translation="MKKNLLRAAVIFTLLAGSTAIYAQAPQEQSGGQWGHGQGQPMTV
                     DQRLQNMTKQLNLSDAQQQQIKPILENEAKQMQSVREDTSLAQQDRRTKMMQIRQETS
                     SQIKPILNADQQKQYDETMMRMNRGRGAGGPGQPLPPLPPSASQPPQQ"
     gene            complement(39764..40477)
                     /locus_tag="VFC15_09595"
     CDS             complement(39764..40477)
                     /locus_tag="VFC15_09595"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HZM10450.1"
                     /translation="MLSWLMRQSTSGQRLERDNGAKRQPGRPRSEHARHAILRSTLKL
                     LQRSGFPDLSIEAIAADARVGKTTVYRWWPDKGALVVDAFASSTETKLHFPDTGSVYR
                     DMSLQMNQFLGILRTRRGRIVAELLGAGQADPKLLEAFRERFLRPRRQEAYKTLQRGI
                     DRGELPKTLDLDIVLDVLYGAIYMRFLIRHDELSESYVKEVCHLVLGNVAANSNAVGQ
                     NHGRKKLEIPIIIARESRS"
     gene            40586..>42762
                     /locus_tag="VFC15_09600"
     CDS             40586..>42762
                     /locus_tag="VFC15_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM10451.1"
                     /translation="MWIVALALRRPYTFVVMALVLLILTPIVILRTPVDIFPDINIPV
                     ISVVWNYGGFAPSEMADRIVTNSERGLTITVNDIEHIESQSVNGVGVIKIFFRPQANI
                     QLALSQVTAMVQTTVRGLPPGTTPPLVITYSASSTPIVQLGLSSKTLPEQQLFDLGQN
                     FLRSQLATGHGAATPYPYGGKIRQVQVDLDIPRLQANGLSPNDIVSAINSQNLITPSG
                     SAKIGSLEYQVEMNSSPQTIAELNDLPVKTVNGSTIYLRDVAHVRDGFAPQTNIVRQD
                     GVRGTVMAIYKIGSASTLSIVAAVKGVLPIAAQSLPKDITIKPLFDQSLFVRASINGV
                     IREALIAAALTAMMILLFLGDWRPTIVISISIPLSIFVSVIVLSALGETINIMTLGGL
                     ALAVGILVDDATVEIENIERNLAQGKEMKQAILDGAAQIAVPAFVSTLCICIVFVPMF
                     FLTGVARYLFVPLAEAVVFAMLASYFLSRTLIPTLVMYIMRGHEHRADGPKTIFGRYQ
                     RGFEASFEKFRRGYYQLLETTLGHSKVFAACFLAFCVLSMGLVFFLGEDFFPSVDAGL
                     MRLHVRARAGMRVEETARLCDEIEKYLHQQIPKDELVTILDNIGLPYSGLNLSYSNSG
                     VIGTSDAENLVGLNPEHHHPTGEYIRTLREELPRRFPGVEFFFQPADIVTQILNFGLP
                     APIDIQVTGADMQGNYFIAQKIANRVKQIPGTADVHVQQMLTLP"
BASE COUNT         9205 a        11892 c        12676 g         8989 t
ORIGIN      
        1 cgcctgccgc gggataattg tcaattcacc ttgcgtccaa atctagatca ccccgtaact
       61 tgctgctgaa taagagctta taggcatgta tcacgccgta acatgagtcg ctatgatgtt
      121 atagtagtat cccgccacgt attaattagt atggatgcta cgagtgatcc gggttcgata
      181 gtccgctttg gggtcttcga ggtggacttg cgagccggcg aattgcgcaa gaacggcggc
      241 aaggtcaaag tgcaggacct gcctttccgc gcgttgaaac ttttcctgtc gcggccgaac
      301 caggtgctta gccgagagga attccgccag gcgttgtggc ccgacggagt gtttgtcgat
      361 ttcgaccgcg gtatcagtag tgcgatcaat cggctgcgcg agtccctgtc cgacacggcc
      421 agtaacccgc ggtttatcga gacggtggcg cgttcgggat accgttggat cgcgccaatt
      481 caggttgtca gtgcgtcgga tgagcgcgtt gccgatcagg gcattggcgc gcgacgtccc
      541 gcgcaagtgg agcaggtcgc ggtagctccc gccgcgcgaa acttctcctt aaagaagatc
      601 tgggtgctgg cggctggggt cgcagtgctg gcgatggctg tagttctggt gatgaagtgg
      661 cgtcccagtt ctgtcacgtc tgcgagaaac aactccggag ttactgcggt tactacggtg
      721 agtgccccta gtccggaagt cgaggaactg tacctgaagg gacggtatta ctggaacaag
      781 cgcacacccg aggcgctcaa ccatgcggtg gactactttt cgcaggcgat cttgcgcgat
      841 ccgaactatg ccaaggcgta cgtgggattg gcggacagct acaacttgct gcgtgagttt
      901 gcttcgatgc ccgagtccga agcgtatgcg cgggcctttg tggctgcatc gaaggcggcg
      961 gaactggatc catcatctgc cgaagcccat gccacgctgg gatttctcta cttctggtcg
     1021 aagcgggacc tggctgcttc cagccgtgaa tttgagcgcg ccattgcgct gaatccaaac
     1081 tacgtcgatg cctaccattg gtatgggaac gtactggctt cggcgggccg aagtgaggag
     1141 agcttgactt atctgaaccg ggcacaagaa ctcgacccgg cttcgccgtc gatccgtgcg
     1201 gacaaaggta tcgggctggt tgccgcggga caccgggatg acggggttgc gttgctgaaa
     1261 cagatggagg cgaccgatcc caacttcatt tcgccgcact cttacctggc cgcgatttat
     1321 ctgggtgaaa tggactgttc cgggtacctt actgaggcgc gcgcgcacgc tcgactgcag
     1381 cgagatcctg ataagttggc aatggtgcaa gccgcggaga agggatttgc tgccggagga
     1441 tgtccggtca tgctgcggag cattctcgag gtgcagaaga agcgctatgc gcagcatcgg
     1501 gtaacagcct acgagctggc tgagacagag gcgttgctcg gcgataaacc tgcaacgttg
     1561 cagtacctgc atttgtctct ggcccaaaac gaggccgcat tggccaacct gcgcgcgtgt
     1621 tcctattttc gcggcctcta taccgagccg gagttccggc agttggttct cgacacgggt
     1681 ctgccgccgc tcacctgatc gcggatttgt cgccgcgaat accctcgtcg taatgtgatt
     1741 atcgatggtc aggaggcgcg ctggaacgtt tcattcacgc gcagccgggc gttgtcggtg
     1801 tcctgaaggg agggagcgcc gatctggagc attccattgg tattgggctt acagccgtgg
     1861 aagaacaggt tctgattcat catgatggat tgcaacaggc ggcccagtgt gcgggagtga
     1921 tagccattgg agaacgatgg tccgtcagac cagccgggat tgaccgagta gttgcaaatc
     1981 aggctccagt attcacgggc ggaaccccag atggtcaagt attcgacgga gccgccggga
     2041 ccgatgtgat attcgatcct gaccttcccg cctgtcggtg acgcggtcag cgcctcccaa
     2101 ttgagaaata tggcgcttgt caggatgtgg tcaagctgga cagggttgac gggcttcgcg
     2161 ggggactcag gtatcgctgt ttttgtcatt gagatttcaa tcttccttgt gagcatgatt
     2221 ggcaggagtt ctcctgctgc gagctaagtt cagaattccg attcaactag taagaggcgc
     2281 aagagacagt tcaaactaag cgtccagcga atcagcgatg aaatcgagtg ggctgatcgc
     2341 cggtacactg gattccaaga cgttgtgtgg ggtgccgttc atttttcttc ccgccgcaat
     2401 ccgccaggtg gcttcgacga ggccattgcg ctggagcagc gagtcacaac tttcccgaac
     2461 tgccttacgc atcgaatctc cccactgcga gccatgagct tcagcaatgt aggagcctag
     2521 caacggctcc aagtcgatga atttcggcct gccgaccagg aaggcgacga tctggcccgc
     2581 gctatctttc ttaatctcat cgcgaaaggc caaacatccg cggtgatcgg cgcgaatgtc
     2641 gatgagcacc atgtcataac ggtcacgctg ccagatggcg ccggcctcgg ttacatcgct
     2701 ggctccgatg acttcgaccc cgtgggtcgc catgatcttt ttgcgcaagg cgcggcgctc
     2761 ggagttggcg tcgatcagga ggatgctgag cttgggtgca agagttttcg ttacaggctg
     2821 ctgtcgcccc tgattcatga cgggtaacct cgcgcgagaa acgggtggct gacaaaaatc
     2881 tgccgctgcg aatgcaagct cggcaggctg ccagaagaaa actggtgagg agggctgaag
     2941 gtttcgcctg ttactgggag ccaggaaaag acgaagtcat ctttgctttg taacgatacc
     3001 tttattatac cccacattct gaaacggccc ggcggagacg attgcttcag gaaaggtggg
     3061 agcccgagga tggggacaga ttgccggagc ggtcttcgaa gggaggtcca agaaatggag
     3121 tttgcataaa ttgtgaggtc acacggcgag gcggagcact atacaaggtt ctttccagaa
     3181 tagataattg cgcaacgagt ccatgcgcgc aaaaaacaac cccaaaaagc ctgttccgat
     3241 gctcgccatt acggtggccg ttctcgtcgc cgcggtatgc gctgttgtag cgtggcgctg
     3301 gtccaccaga gtgcatggcc aggaaaccaa gaaggcctcg tcgtcaacgt cagctgcata
     3361 tgttggccag gagcgatgcg ctcggtgtca tgcggagcaa gagcaggcgt ggcgaacgtc
     3421 tcatcacgcc caggcgatgc aggtggcgaa cgattccacc gtactgggaa atttcaacga
     3481 cgcccgcttc gcgaaagacg gtgtgacatc cagcttcctc aagaaagatg gcaagttcta
     3541 cgtccgcacc gacggccccg acggcaagcc acaggattac gatctgccct atacgtttgg
     3601 cgtatctcct ctgcagcagt acctggttcc attccccggc ggcaggatgc agagcttcgt
     3661 ggtggcgtgg gattcgcgaa gcaaagatca cgatggtcaa cgctggttcc atctctatcc
     3721 cgaccagaag attggatcca cggatccctt gcactggacg gggcgtaacc agacctggaa
     3781 ttacatgtgc gccgattgtc actccaccaa tttgcgcaag aactacgacc tcgccaaaga
     3841 cagctacgac acgaagtggt cggagatcga tatatcgtgc gaatcgtgtc acgggccggg
     3901 atcgaatcac gtggcatggg cgcagacgca caagaaagga tcgtacaatc cgggcgaagg
     3961 atcgaacggc ctcgtggtcg atctgagacc ggccagcgga tcgtgggcga gtttggaacc
     4021 cgacaccgga accatgcact ggaaggggca ggcgcgctcg cgcaacgaga tcgacacatg
     4081 tgccccgtgc cacgcgcggc ggcatcccat caccagtgac aacgagcccg gacagccatt
     4141 cctcgatggg tatgtgccga gcttgctgga agaaggtgtc tattacccgg atggccagat
     4201 tcttgaagaa gactatgagt ggggctcatt cattcagagc aagatgtatc acgaagcggt
     4261 aacgtgctcc gactgtcatg acgctcacac tgccaaactt gctccggtga gcctaaacgc
     4321 cgtctgtggc aagtgccatt cgctacagaa attcggcggc gagcagcatc atcatcacaa
     4381 ggcagagagc gcgggcgcgc tgtgcgtcag ctgccacatg gcgaccaaga cctacatggt
     4441 ggttgacgtg aggcgcgacc acagcttccg cgtgccacgc ccggattttt cggtcgctta
     4501 cggaacgccg aacgcttgca cccagtgcca caaagataag tcgaacagtt gggcggtcga
     4561 tacggttgtg aagtggtacg gccccaatcg caggcaagaa gcgcaattcg tgacagcgat
     4621 cgaggcgggg cgtcgcggtt tgccgaacgc tgcgaaagcg ttgactgcct tgattacgga
     4681 cacggcaaag cctggcattg cccgcgccac cgcgttgagc cttctgccgg cgtatctcac
     4741 tccgtctgcg ctgcctgcag tgcaatccgc actcgtggat aacgatgcac tggtgcgcag
     4801 tcaggcggtg cggtccctgg agccgctgtc gccgaaggaa cgggtgcagc tggctgcgcc
     4861 gttgctgacc gacccggtcc gttatgttcg catcgaagcg gcgcggctgc tggctggaac
     4921 gcctgcagag cagttgcagg acgcgcagaa gacggcgctt gaccacgcga ttgcggagtt
     4981 gatagccagc gagatggcct cggcggaacg tcccgagagc catatgaacc tggcgctgtt
     5041 gtatgcgcag atgggccgcc cgaatgaggc agaaagcgaa ttgcagaccg cgttgcggct
     5101 tgatcccaag ttcgtgccgg ccatggtcaa cctggccgat ctgtatcgca cccaacagcg
     5161 cgagcaggaa ggacagcaat ggttggaaag ggcaatcgct gtcgaaccta atgcggcgga
     5221 gcccgtctat gctctgggcc tgctcaaaat caggcagaag aattaccagg atgcactgat
     5281 gctcttggcg aaagcggcca gcttgcagcc caacaacacg cagtatagct acgtgtatgc
     5341 ggtggccctc aattcagcag ggcacgcgga cggagccatc agcgtattgg agcaagcgca
     5401 tcaaaagcgt ccggcggatg ttcaggttct ggacggttta attgccttcg agcgtgacaa
     5461 ggggaatctt ccggcggcaa tcacttacgc gcaacagtta gttcaactga ctcccgacga
     5521 tcccagtgcc aaggcgatgc tggcgcagtt gcaacaacaa gtccgacagt agaacgtcgc
     5581 gggaaccccc ggccaattat ttcttgccga cagcggctat tttccggaac caactggtga
     5641 ttctccatga cttggactta tacatggagg ccagggctgc ttctttttcc tgcaaagccc
     5701 cgttcaggcg ctcgattgca atggattgct gctttacctt tgtctgcaat cccgtcaact
     5761 gcgatgccgc gaattgttcg ccatcgctgt ccggcctgaa tggcttgatc tcggtcgaaa
     5821 ctatggtcgt gtggcgatcg tctagagtga tcgcaagcgg tgctcgctct gcagcatcat
     5881 acgggttgca agcagggatt tgtaattaac cctatcttct tgggagtggt ggctaccggt
     5941 gtcagggacg atttgaatcg tcgcctggcc ttctggtcca aagggcaatc catcttgatc
     6001 catcctgaag gtgttgctcg aagcccggca gtcgctcatc gagttcttcg gccagccagc
     6061 ggccgaatga cttggtttcg gtgatggcaa gcttgcagaa ctcggtcccg gagcaggcga
     6121 tgatgccgcg ccggaacggc gagccttcgg tgggcagtcc cacgagttgc agcgcgcctg
     6181 cgaggatagg cgcgttgcgt tcgggcacgt tgacgatcag caggttctgc atgttggtgg
     6241 tgcgtaattc gcccgcggcg tagcgctcgg aaagatcggc tgccgcgtgc agttgggccg
     6301 ccgaaatccg gccgcgcaga accgaagcgc ccacgtagca gtagccatgc tgtttctggc
     6361 ggcgtattcc cacgtgatcg cggtagatgt cgtcgggaat ttcttcggga acggccggag
     6421 gcagttcgaa tccaaggcac cctttcagtt cgctgagaaa gtggtcggct gtccagccgt
     6481 gcttgaggaa gaggaatttg agacgggcgc ggtcacggtt ttcgcgcaat acctcgctgt
     6541 cgcgaaagat gggagcgatg cccttcagta ccggcaatac ctgattcggc aggacaaatg
     6601 cgtcgactcg tttagcgaga tgagggtcgg tcgataagcc tccggcgacg cgcaaggaaa
     6661 atccgacctc gcggccattg cgacgcaccg cggtcaggcc gacgtcgttg atctcgggat
     6721 aggagcacca ggcgtggcat ccggtgatgc aaatcttgaa tttgcgcggc aggttgtaga
     6781 actcggagtt gccgacaaat tgccggttgg cctggttgac cagcgacgac gcgtcgtaga
     6841 gttcgttcgc gtcgacaccc gcgacaggac atccgataat gttgcgcacg tcgtcgccgc
     6901 acgagcccat ggtggtaagc ccagcccgcc atagcgcatc gagtacgttc ggcaggtccc
     6961 caatcgtgac ccagtggagc tgaatgttct ggcgaaccgt gatatcggcg ctgccgcggg
     7021 cgtacttctc ggttacatcc gcgatggtgc gaagctgatg cgaaagcaaa aggccattgg
     7081 gaatgcgaat gcgcaccatt gcgaatgcgc accatgaagt aagggacggc cttaccttca
     7141 ccgcccgcgc caccgagggc tccgaggcca tcgccctggg tgtaaacgcc ccagaaacga
     7201 aagtacgtgc ccaaccattc cggaggaatg gattcatgtc cgctgcgagc gaacgcacgg
     7261 atttcgtcga ggcactccca ggcgttcttc tcccgcttga gccgctccat tcgttgcgcc
     7321 ttagtttcct tcagtgcttc cggcatcgac aacctcttta cgccaaaaag aaaaacccac
     7381 gtgtcagata ggtctgacgc gtgggttagg gaaatcctgc ttaagtaaga aagctagctg
     7441 gtatttccat cggcgtcaga acatgtgcac ccgcaacaac agcaggccat gcaacaacaa
     7501 ttctgacgac cactcaacat gatcgtatga cactacacaa gttgggagag caggtcaagc
     7561 ggttgcggga tgaaggttgt gtcgcctgtg accaacgcat gacggagagg tacgatccag
     7621 ccgagtcgtg cgccggaccg atgccgactg cagcgtacga caggctacgc gggaaccccc
     7681 tgtcgcgctt tatagacaca ggccggacaa aataagctcc taacgtccca ctatggaacg
     7741 tatttgcagg tattcgtgaa cttggtcgaa tatttaggct aaagtccttg ctattagcaa
     7801 ctcttctgag ctataaaccg cctttgtgtc gccattttca ggaggtccgg tgaccattcc
     7861 ctctcgtgcc attcgttatt gcctgctgtt tcttgcaaca tttttggcta gctccctgct
     7921 tctgaacgcc ggtacggatt gggcgccggt ggatccagca gagctgcaga tgaaggacct
     7981 gcctgagcag ccgggggccc cggcattcgt tctatttcat gaagagatcg acaacgatca
     8041 gcagggatct cactcggtgt atatgcgaat caaagtgctt tccgaggcgg gacgcaagta
     8101 cgccgatgtg cagattcctt attaccggga agcattcact atttccgatg tgcatggacg
     8161 aactattcat cctgacggga gcatcgtcga gtttcaggga aagcccttcg acaaagtcgt
     8221 agtgaaaagc aagacgctga aggtgcggga gaaggccttc actctgccgg acgttcagac
     8281 tgggagcatc ctggagttca agtacatact gcggtcggag aacggcttgc tattgccgcc
     8341 gcgctggacg gtacaaaacg atttgttcca gcgcaaggag catttcaagt ttgtcccata
     8401 cgcaggaagg atacagatgg cccatggcga ggtcggcacg ggactggcct acacctggat
     8461 gctgccgaaa ggcgctgagg tgaaaaacac cagggacaac tatgaacttg atctcgcgaa
     8521 catgccggcg ttcatcgagg aggagcacat gccgcctccg cagcagtata agtactacgt
     8581 tcgcttttac tacaagcggt tgagcaatac cgatcagtat tggaaagaag agggcaaata
     8641 ctggaactcg gacgtggaga aatttctggg caagaaacac ggcatcccgg aggcggttgc
     8701 ggggattgtg tccgccagcg atacgccaga gcagaaagtg aaaaagatct acgcctacgt
     8761 cgcggcactc gacaatctca gctttaagcc gcaccgcacg gagcaggagc agagagtcct
     8821 gggcgtgaag gacaatcgag gagtggaaga tgtgctccgg cagaggtcgg gcgaccggca
     8881 ggagatgaca ctgttgttta ttgcgatggt acgggcggcc ggagttccgg cgtatgcgat
     8941 gtatgtcagc gaccgaagtg aggatgtatt cgagaaaact tacctcagct tcgatcaact
     9001 gaacgccttc gtggccatcg tgcagatcga tgggaaagac gtgttcctcg atcccggtac
     9061 gaagttctgt ccctacggcc tggttgattg gaaatataca ggaacgcgtg ggatcaagca
     9121 gttgccgggc ggaggcacgg agatcgcgga gactccgccg cctgactaca tgaaggctat
     9181 tatcaagcga gttgcccgct tgcaattgaa cgaacagggc caggtggaag gatcgctggc
     9241 gattggattc tttggacagg aggcgctgac ccgaagagtt gaggcctcca aaacggatga
     9301 ggttggacgg aacaagatcc tggaagacga ggtcaagagt tggctgcctt cgaacgccca
     9361 ggtcaccctg accaagccgc cggagtggga ggcggttgag accccgttgg tggctgagtt
     9421 caagatttct tcgcccatgc tcatgtcggg cgggaagcgt gtactgttgc cggcaaacgt
     9481 atttgagttc aaccggcctc cgatgtttgc tcacgctgaa cacacgcacc cgatctactt
     9541 tgagtatccc agccgcgaag ttgacgatgt gcatatcaag ttgccagtca acttgcaggt
     9601 ggaaagcctg ccagccaacc aggacgtcaa aatggagtac gcgctctatc gggcaggtcg
     9661 caagcaggaa aagaacgaga tcgacatgac acgcgacctg gcgatcgggg cttttgtatt
     9721 tagccccacc gaatacaaga acctgaaatc ctttttcgac aaggtgaagg aaggggacga
     9781 cgaacaggtg cttctgaaac aggtggccca tgtcgcgcaa aactaggttc gcggttaccg
     9841 ttctggttgc gctggcttgc tcggcgccac tcctgggctc gtctcccagc gcgcctgact
     9901 ggcttcatca agccgcagcg aaggcaccgg ggaaatatgc acccgagaca aacgccgtcg
     9961 tcctgctcga cgacacaacg ctgactgtaa ccgggcctgg ccaagctgac gagacacggc
    10021 ggcgcattgt gctaatcctg cgtccgcagg ggcgcgagga agccaagctg catgtctttg
    10081 ttggaccggg tgacaaggtg caggccatcc atgcgtggac gattgacagt gccggtgtgc
    10141 aatacgaggt gaaagataag gaattcatcg aggtcagtcc ctacgaaggc gccctgtata
    10201 cggatattcg ttttcggacg gcggaggctc cggcgggcaa tccgggcagc ttgattggat
    10261 gggagtacac cactcgcagg cacatctgga tggaccagtg gcactggttc gtgcaggaaa
    10321 atattccggt ggaagagacg cgactgacgc tgcagctacc tgcgtcgtgg gagtacaagg
    10381 ccagttgggc caatcaagcg gcccagcagc ccgcacagac gggaccgaac cgctggcagt
    10441 ggatatgcct gaatttgccg gggattcctg aagagcatct gcgtcccgct tcagaagcac
    10501 tagagggcca cctggagctg gcattctatg aaccagccac cgcctcgaac ctgggttcgt
    10561 ggaaggcgat cggtgactgg tactacaagc tggtcgatgg ccggcggaat ccgtcgccgg
    10621 aaatgtcagc gcgggtgcaa cagctaaccg caggggccac cacttttgac gggaaggtcc
    10681 gcgctctgac tgcgttcctg cagtcggatg tccggtacgt cgaaatctcg atcggaattg
    10741 gggggtatca gccgcatccc gctgcggacg tatatcggtc gcgctacggc gactgcaaag
    10801 ataaggtgac gttgctgagc agcatgctga aagaagctgg tatcgactcg gagtatgtgc
    10861 tggttgatag cgagcgcggc gtggtcaagc ccgacgtgcc ttcgacgctg tttaatcacg
    10921 cgatccttgc gatcgatctg ccgaaagacg tccctcctaa cgcctatagg agcgtcagca
    10981 caaccacctc tggcacgcgc tacttgattt ttgatcccac cgacgaatac acgccagttg
    11041 gcgatctgcg caccgcgttg caggggagct atggactgct ggtgacgaag tcgggcggcg
    11101 aactgatcaa gttgccggtg ctgctgccgg acaccaatcg cttcgaccgg gaagggaaat
    11161 ttacgttgca ggctgacggg tcgctttcgg gaacggttac cgaacggttc acgggcacgc
    11221 atgccgctcg tcaacgcgca tggctgggca cgcagaatga gagtgaacga gcgaagtcgc
    11281 tcgaccatcg tctaggcgaa tcgctcaaga acgtcagcgt gcaacaactc aaggtgcaag
    11341 atcttgcggc tcatgacaaa gaactgacac tcacctacca gctcacggcg cagagctatg
    11401 cccagaagac cggaccgctg gtactggtgc gggggcgggt gttgggtgag aaggcggtcg
    11461 agatcaactg ggcaaagcgc aagtttccga ttcaactctc gggtgcgacc tcggagaaag
    11521 acagcttcga aatccagatt cctcccggat tcgcggttga tgatctacct gaaccaaagc
    11581 agatcgacgt cggttttgcg agttacaaga gcaaggtcga ggccaatgga tccacgctgc
    11641 actatacgcg cgagtacgtg gttcgcgatc cctacatagg atcggaccac ttctccgacc
    11701 ttcgcaaact ggagggaact attggcgacg acgagttcgc cagcgcagtg ctgaagaaga
    11761 cgccgtagcg cggccgacgg acacttgcac tgcgacgatt aacagtgaac tcgcgacact
    11821 attttggagg agccaatggg gtgaccggca cttccgcacc cctcaccgca accagatatt
    11881 ccacaatcag tgcctggtcc gcttccgaga tgggagcacc ataggcatcg accattttct
    11941 tgacctcgtt ttgccaaact gatttggaaa accgaggctg cgtcgaaaca tagcgcgccg
    12001 tatggcaaag caggcagttc ttgcgatagg tctccaagtt gggtccattc gcgatctgcg
    12061 gcgggtattg tggaagcaca atactctgta cctggctggg gttggccttc gcgggtggtt
    12121 tcggtggatt agccgagggc tgttgcgagt acatcgcgac ggaacccgtc aggcacgttg
    12181 caatgacggc gaacacgatc ttccagtagc tactcgatcg cctcatacga ctatcacatc
    12241 gacgtgctcg atcacgccac gttgataccc gctacgattc cactgggcgg cgggttgcgt
    12301 ttgtccggca ctattggttg cgcgtaccaa caagcgatac gaccctttgg caggcggggt
    12361 ccagcgcgct tgccatctgc gccaggagta ctttcccaag tcgatgccga ggattgcgtc
    12421 gctccaggtt tgtccaccat cgaacgatat ttccacgcgg cgaatcccat cacccccgtc
    12481 gttggcgact cccgcaagtt catagctgat tcctgcgtga agttgctcat cgggcgcggg
    12541 gcgcacgaaa atcgaatgca ctgacatgcg attgatggga atcgttgcag gtgaaagacg
    12601 caggggatct tcgttggcat tagagttgtc gggaatccgg tacgccttgt ccatccagaa
    12661 tgtcttcaac ggctgctcca gagcagtgat ggacgacaag gatttcaccc agtaggtggc
    12721 ataccagccc ggaacgacca aacgaagcgg gaatccgttc aacatcggca ggggttcgcc
    12781 gttcatttcg tatgcgatca aaacgtcact gtttcgggcg cggtcaatcg gcaacgattt
    12841 ttcgaaacgt ggtgtctttg gcattagggg aacgtcgagt ccgtgtagcc ccacttgaat
    12901 ggtgccggga agcacgcctg cagcacccag aacgtccttc agccgcacgc ctttccagcg
    12961 agcattgccc atcgcgccgt tggcccactg gcctccgggc actctgggct cgaagaggct
    13021 ccgtgaattg ccggcgcatt ggcacagtgc cactagcgag accggctcga acttcgactt
    13081 caagtccgtg atggacaggt tcaggggctt gctgacatgt ccgcccacct ccaaccggaa
    13141 agaccgcaag tcaaccgtgg tgggaattcc cgcgagatgc cagcgcacga agaaggcatc
    13201 gttgggcgtc agatcgttat ggaagtggcg cagcggagtt tccaactgcg gcggacgatc
    13261 agtgagcaaa atcaggtccg tcttctgagg atatcgaacc agttggcggg cggcaggttc
    13321 agtgggaaga gtttcaaatc ctggaccaga cgatgcttca gcaggaaacc ccaaagcgcg
    13381 gcgccctgca gcgaacccgc caagccaagc cagcagcgac acgaagcgac gccgatcgat
    13441 gcggctagag tgagtggtca gcatcgttcc catggcttct ctcaaaagaa agcataaacg
    13501 ggagacgaag gcgctagcga gatctttcca caggtcgggg agcgaattca aggcacaacc
    13561 ggaaagaagg cctgcgacat ttagctggtc cgccgcaaca ttcaactgga tgaagcgcgc
    13621 gggaatctag cacgccaaat gctcaagcgc ggacatattc tcgataagca gggtcgaacc
    13681 gtggagcacg gcgatgtggt gcttgcgaaa atcggcaagt gtccgagtga ccgtctcgcg
    13741 cgacatgccg ataatctgcc cgatctcatc gtgggtcaaa gccaccttga ttccgtggga
    13801 ggtggacatg ccagccccag cgagggacca ttccagcatc aggcgggcga ttcgctccgg
    13861 ggcagaacgc ataacaaagg agcgaagttg ctgatacgcc tgctggcatt cgcggcttag
    13921 atgcatggct gcatgcattg aagctcccct gttctcggtc aggaaccgca ggaagtcggc
    13981 ctgtttcaca aagttaagct ggcacgcctg gccagtctct gcagtcgtgt cgtgcgggga
    14041 tccagaaacg gtggcgttta gacccagcac ttccccgggc ttggcaatct tgaagatgag
    14101 ggtttggccc tggctggaag ttgccgacag tttcacgcga ccgcgacaca agatgtaaat
    14161 accccgacac gcttgtcctt cgacgaacag agtcgcgcca gccgggtacg cgagggtgaa
    14221 ctttatcttt tgaaaggcct ccatctccgg tttcggcaga cgacagaaga agccttcgga
    14281 acaaaggttg caggacagac agttttctat cagttccatt ccgtatggtg attgcattgt
    14341 cgccctcgct gtcggaggcc ccaaaagaac ggtagaaacc ctggcagtgg aattggggga
    14401 gtgtctgtgc tctgagtggc cgactgccag ggaatctaca cttatgattg ctgagaacaa
    14461 taaagtgttc gaacggcgct aatctgttca attttgctca cttttagaat tattgttcgg
    14521 agggacactg caggtcctcc gggacaattg gacttggggc ggcctccaaa attgcccatg
    14581 gcattcggca atccttgcag ataacatgca gaaaaataaa tacttggact cgagaattca
    14641 gcattagtgc gcaaggtgcc gtgccgacca gcagcgcgat ttgagcgaga tgggcgcccg
    14701 gacttacagg cttgcccgat ctatgcgcta ggtggccaga ttttgaagag cgggcatgtt
    14761 ctcaatcagc aaggtcgaac cgtgcagcac tgcgatgtga tgcttgcgaa aatcggcgag
    14821 cgtacgcgta accgtctcgc gcgacatgcc gataatttgc ccgatctcat cgtgggtgag
    14881 agccactttg attcccagag tggcggccgt tccctcgccg ccgatcgacc attccagcat
    14941 gaggcgggcg attcgttccg gtgccgagcg catgacaaaa gaacggagct gttgatacgc
    15001 ctgctggcac ccgcggctta gatggatggc ggcatgcata caggcgacgc tgtgttcggt
    15061 caggaaccgc aggaagtcgg cctgtttcac aaagttcagc tggcatgcct ggccagtctc
    15121 cgcagttgtg ttgtgggggg ttccggagac ggttgcggtc agtcccagca cctcgcccgg
    15181 cttggcaatc ttgaagatga gtgtctggcc ctggcttgaa gtcgcagaca atttcacgcg
    15241 gcctcggcac aagatgtaaa caccccggca cgtctgtcct tcgacgaaca gggtcgcgcc
    15301 agccggatac gcgagcgtga acttaatctt ctggaacgct tccatttcct ctttgggcaa
    15361 ccgacagaag aagccttcag aacaaaggtt gcaggacaaa caattttcta ccagttccat
    15421 gccgtacggt gactgcattg tcaccctcct cgcctaaggc cctggttgtt gcagaatccc
    15481 tgatggtggc tgcgaagaca aagatgttcg tggtaagtca aggctgcagc agggattttg
    15541 ctgtggtcac gattaaggat acgctcttaa cgggcgcaca ctgggcaata ccgctcactt
    15601 ctcttaatta atatcgtgcg ggagcaatcg ggaccgctgg cgacttcgca accctcgccg
    15661 cctgggcgcg gggcaaggca gagattcccg gcaagagggg cataaagaaa ggcttcaacc
    15721 cctgggacat caatcgtgga gcaccacgtt caaaagcgaa ctgtggactt gcaatccgcc
    15781 gccgccgtat tcaacgaatc ctagctttcg gaaccggttc atgaagaagc tgacgcggga
    15841 ccgggtggta ccgaccattt cggcaagcga ctcctggcta atcttgggga tcacgatttc
    15901 aggtttgcct tccttgccga agtgcgccag aagtagaagt atcctggcca gccttttctc
    15961 gctggaatta aaaagttgat cgacaaggtc ttcttcgtag cggatattgc gggccaacag
    16021 gtgagagacg aacaagtctg aaaatgactg ctcacgatga agcgcctgca tcatggactt
    16081 cttatcgatt cgaagaatcg tgcactccgt catggccgag gccgagccca ttcgaaatgc
    16141 ctggccggcc agggcgcctt ctccgaaaaa actgccttcc cccaggatgc ctacggtggc
    16201 ttctttcccg gcctgggaga cgacggtcag cttgatcttg cctttctgta cgtagaagac
    16261 ggcatcggct gcaccgccct gggcgaagat gttctgacct ttctcaaagg cgagaatctt
    16321 tcgacctgtc ccgatcgtcg caagaaatgc tctgggctca aacttgcgcc tgttcttcgc
    16381 tctcatcgtg gaagcacccg cccaggctcg gcagtgattt ggaatgggag attatcgcag
    16441 gtgcgggata tttggcaacc ccattgagtc gcgagttcca agccgggcgt tacgctgccg
    16501 aagatgcctg atccgccgac ccgggggccg cacgatttgt cggattgcgc cacggttgca
    16561 acaccaggag acgtcacacc cgaacggcgg cgagccagtc gtcaatttgt aaagtggggt
    16621 agttatcgga tccgcggaag tgcacagtgt ggagccgcgc tatttgtgct tcggtccgtg
    16681 ttttggatcg ggctgcaact ccgctcgaac gaatgtgcct ccacacacgc tgcagaccca
    16741 ctgtgcctgg gcagcactgg gaaccttgct ggagctgctg gtccgccact ttctggaaac
    16801 ttcgctttta cagactgggc acttcaattt aagggggttt ccttccctgg caacgacggt
    16861 aagaactgct cgacagggct tggtggttgg gccgtttctg acggggagcc gcggttcaga
    16921 ttggagctcc gtgcctgctg acggcgacaa ttttaacttt tgagatcaaa tcattgtcgt
    16981 cggccaagcc aagaactcgt ctggcgtgct ttctggcctt ttcctcggta agaaatcttt
    17041 tggccggccg aagaccgggc agcactggca ggaattcgac tcggtagtaa gtcacgcgat
    17101 tctcccttct gttgcgatca cgcggaaacg acaaaccgca tgttggttcc aatgtctcat
    17161 cgccacgagt ttgggacggc tgtccatcgt tgaaaagtcg gcaggtaccg gcgcttgtgg
    17221 cagacaatgc acgttcccgg gcacaaaaag aggcggggca gaacgactct ttcgacccat
    17281 ccctcgggag gcaaggccct gaactggtct cccttacata cgcggcaatg ggcggacaac
    17341 aagttcatgg cagtggagta acggtgtgtt cagtgccgaa cgtctgcagc ggccgccccg
    17401 gtatgtgtcc ggagccatgc cgcccgatat ttcgattttc actcagacca actggagaag
    17461 gctgagcaac attgctcagt gagcaataag gctcagacga gtgcgatgct ggcacgatag
    17521 gctcactcgg tggtccatcc ttaagaagcg cacgcgactg cctagcggag attcgtgcaa
    17581 ggagaaacaa atgaagtggg aacaagtgga aaacaagtgg gaacaattga tcggctcagc
    17641 aaaggaaaac tggggcagga taggagacga tgacttccgc ctaatatcgg gcaagcgcga
    17701 gcaactggca ctcaagattc aggaagccta cggaattacc aggcgagagg ccgaaaagca
    17761 agtctgggac tggggaaaga ccgtggagaa tgtcgcaaag aagatcgcgt gaggtgagtt
    17821 agcgatcgcc agcatgtata accagctcgt cacaggagat aaaagatgac cgccccagag
    17881 acagaaatcc cagaattaac tgcatcgccc agtaaaacag caacgccaaa gaaacagaat
    17941 gcctccgcca ataagcgcgc cgaggacctg ctagcaaaaa agaagaaaaa acgcgctgcg
    18001 caccgggtgg ccctgagaag gccacacgct aacggctaat tatttttcgg agtggattca
    18061 cgtccaggtt ctgagcgacg ccgaaagagc agccgcaggc agacggctgc agttgcccgt
    18121 gaccggttag cgggagcagg attggcggtc caccaacgag gtggccgtcc ctgcagttat
    18181 gctctacgag tttctttcgt ccttaagccc tcgcgcgaaa tcagcttaac tggagtggcg
    18241 cgggcgagta cgtctatggg ccctccgcga ctgccatcag cgcaaaagcg gggctacctg
    18301 taaaacccgg ctcgcaaatc gaatcgcttc ccgcaatgcc catcacttcg cctcacccga
    18361 cccgcgcaaa gacgttgcgt ctcggaggtt acatctgcgg gctttgattt caagttggtt
    18421 tgtaccgccg tccctgactc agctaaaccc tgttcttacc taaacatgta aagccggccc
    18481 acgcaaatga tctaccggcg cataagccct acttatcgcc tccataaata gaacttgtcg
    18541 cgatctttct gatcgtcccc atagaaaata tgagttggac gattcagact ccgtccgcta
    18601 gtttttagtc cactggcgaa tctgccggga aattttcgag cgatgcaatt cagcaagtaa
    18661 aaggagatcg tatggcggag aataccccag ttgagatgaa agcaactctg ggacttacag
    18721 ggttgacgat gaacgccatg gcgctcatcg cgccgggcgc gtttctgtgg ctcaccttct
    18781 tcatccaggc aaccaccgga gtgacggcgc cagcgatgtg gctcggcatc gtcctcgcgc
    18841 tggcgctttg cctggctacc gcggtctgct acgcggagtt ggcgaaactg tatcccggga
    18901 ccggcagctc ctactatttc gccgaacaat cgtttctcaa ccacgacaag gcgtggcgct
    18961 atgcgcggct ctcgaagttc attgtgggat gggcctccca cctctactac tggatctacc
    19021 ccggagtgat ggtggctgtt atgggcatcc tctgcggata tctggtcgga accatctggc
    19081 cgagcttcat gagcgcctcg aatccgggag tcgcgttcat gatggtcgtt gcgatcgtat
    19141 tctcctttgc ggtggcctat atcgctcacc gcggagtcac cggttctacg gcggtcagca
    19201 tcgcgatcaa cgtcattcag atcgccgcat tggtactgtt cgcagtgctg gcgctcggct
    19261 accgcatgaa ccatccgccg ggcaccgtcg cttaccagtt cgattcggtg tctggtgagg
    19321 cctacaccta cgagttcgca accaccacga ccttggtcga cggtaaaccg accgatacca
    19381 tcgttcgcga ttccgccggc gtggtaccgc agcccaagct ggatacggcc ggtaatcctg
    19441 tgccctttaa gatcagctac cccgagacgg atagctcagg cgcgtttctg tcgcatccca
    19501 acgccaagtc ggtcatcagg gcgcacaact ggggctgggt atttattcag gccacagtcg
    19561 cgatcctgat cctggtgggt ttcgaatcgg tgacgtcgat gggcggcgaa gccaggaacg
    19621 ccaagcgtga cattcccatc gcggtcattg tctcgcttct ggtgcagggg ttgttctgtt
    19681 atctgttcga gtattttgcc gccaattact ttcttaatag cgggtactcg atggcgaatg
    19741 cgtcaggttc agcggcgccc ataggcgaca tgatggtcgt agtgggcgac gcgttgctcg
    19801 gacccgggca cggccggacc ttcatgctgt gtgaggcgtt cacggttttc cttgcgctga
    19861 ttggcaccac gctatcgtgt atgaacacgg gagcgcgcgt gacctacgcc atgggcaagg
    19921 acaaggaagc gcccgagcac ttcggcatgc tgcactcgga aaacctcagc ccgcaccgcg
    19981 ctatctggac actggcggtc atttcagcgg tggtagcctg cctctctctt attttcgtct
    20041 ttggcgatgc cggtgcgccg acggatgccg cgattcaagc cctgccgcac ggtttctggt
    20101 ccagcttcgg atacatcagt cacgaccggg aagcagccat gcctaacaca ctgctcacga
    20161 tgacgctggc ctcgaacttc ggaacgttcc tgttgtacgg cctcagttgc gtcatctgcc
    20221 tggttggcta tcataatcat ccgaagttca agctggtgcg ccacctggtg gttccggtat
    20281 ttggactggt cgccaacctg gcctgcatgg ccttctatct gattggtccc ttcatgggtt
    20341 acggaaccaa gaaagaacct ctcctggctt tgggggtcgc gttggtttgg ttcatctatg
    20401 gcgggatcta ctttatccgc tccagtaagt cgactgggcg gaacacgctg attgaacgag
    20461 gagttagcgc gagcgtggtg taagagcaac gaatcaacgg acgtacatgg gcgggcatgg
    20521 gcccgccctt tgcctgtgcg agtactaaaa tagaaccctc cccgggattg cacactgctt
    20581 actccggaga gtcgggcgca gtactgcgac gtgtattttt cagggcccca tgcttgacgt
    20641 cgaattcact cagattacgg atccgggacg ggtcagggag cacaacgaag actacctcgg
    20701 ctatgtgctt ccccacgttg cagcgcagtt gcgttcccat ggctgtttgt tcgcgctggc
    20761 agacggagtt ggcgggcaac agaaagggga agtcgcctca cgcctcgcgg tggaaagtgt
    20821 gctggatggc ttcgtgagag cgcgggaagg ggagccacac aaggccctac taactcggct
    20881 ggtgcaggcc gccaacaccc aggtgtatga gatgggtgga tcgtcgggtt ctcgcaccgg
    20941 aatggccact acgttggtcg cgtgtgcgct ccggttcgat catgccgtgg tggcgcatgt
    21001 cggggactcg cgctgttatc tgattcgttc tgggaagccc aagctgatca ccaacgatca
    21061 cacggtcgtc aacgaacagg cgcgcctggg gatactttct gcgcgcgaag ccgcggagtc
    21121 gaccaataag cacttactga gccgatcggt aggcaatgac atgttcgtgg cggtcgagat
    21181 caacgatgta caggtggaga agggggacgt gctggtgctg tgctctgacg gacttcatgg
    21241 ccctgtcccc gagccagaca tcgcgagcat agtaacggaa aatcaatatc tttccacagc
    21301 ggcggagaga ctcatcgctc tcgccaatca acgagacggc agcgacaacg tcagcgtgca
    21361 attgatccgg gtccacgacg tagagcgcac agggatgtat cgcggccggc cgtacaagat
    21421 tcggtgagca gctctttcgc tcacacccgc ggattgccag cgcccgtgtg acttgtaacg
    21481 cgccattatt cgaacaggtg cctacgcatt ccttatggaa tctttacgcc gaatgcccgt
    21541 cttttctggc attattgagt tgcgctttcg atcgaccaac aagtttgatc cgagcctcgc
    21601 attgcgaagg aattccgcaa tccgggaacc atgactccag tccgaaccga cgaacaactc
    21661 gatcactatc gcattgacca gctgattgcg cgcagcggga tggcgtcgat ttaccgcgct
    21721 accgatctcc gcaacgaacg tcaggttgcc atcaaaattc cccatccgga aatggagagc
    21781 gatccactgc tgtacgaccg ctttcgtcgc gaggaggaga tcggcaagaa catggaccac
    21841 ccgggggtga tgaaggtctt caccgacgat caccgcagcc aggtttacat ggtcatggag
    21901 tgggtggacg ggcggctgtt gcgggaaatt ctaaacgagc aaaagaaatt accgcaggaa
    21961 cgcgccatta aaatcacgac gcgcattctg gatgctttgc agtacatcca cgatcacggc
    22021 atcgtgcacc gcgatttgaa gccggaaaac gtgatggtcg atgccgacga caatatcaag
    22081 ctcatcgact ttggcatcgc gtccgacctg cacggccgcc ggttgacgtt tgccaacctg
    22141 tcgcaaacca tgggaacgcc ggactacatc tcgccggagc aggtcaaagg taaacgcggc
    22201 gatgcgcgca gtgacctgta cgcggttggc gtcatgctct atgaaatgct ggcgggcaag
    22261 gtgccgttca cgggaacaaa tccttttctc atcatgaacg accggctgct gaataatcct
    22321 gttcccccgc gagagcttga tccagcaatt tcgccacagc tgcaggaagt gatctatcgc
    22381 gccttggagc gcgattggaa gaaccgctac gcgcgggcca gcgaattcgc ctgggacctt
    22441 gagcacctgg accaggttgg cgtggccgag cgcgccgaga tgcgcgactg gaaaacgcgg
    22501 cgcagcccct ggcccagaaa aatcgccttc tacgttgcca tggccctgat cccagttgtg
    22561 gtgtttggcc tgctgctttg ggtggcacgg catagttgat cgggccgccc ccaagggtct
    22621 ttgactgctc cctcgaagtt agctctaccg ttggatgaat tcgtccggat gaaatacgaa
    22681 ttcgggatag gccccggtgc ccaactcgta gaggtccatg ccttgccgct cgccctcggg
    22741 atcgacccgc tgccgataga agcggttgag cagccaattc agagaataag tcagcggtaa
    22801 cacgaaaccc agaagggttg cgattccaat cacctgggcc agccattgac cgtcggccga
    22861 gttccggcca ggaaaacggg cgaacaatcc ggccgctaac aaaccccaga ggccggccac
    22921 tgcgtgtacc gggatggctc ctccgggatc gtcaaccttg agccacattt caaaccactc
    22981 caccgcgaac gtcaccagga cgcccgcgat gaatccaatc agcagggcag ccgccggttt
    23041 gaggaatggc gaggccgcgc tgctcgccac caaaccgcca acccagccat tggcgctgat
    23101 ggaggcatcg ggtctgccga atcgaatgcg ggttatggtg acggccatca atcccgccgc
    23161 agcggctgac aaagtcgtat tgacggcaat cagtacgacg cgtccggctg gaatgtgatt
    23221 gaagagcagt gctcccgcgg aattcagacc caaccagccg agccacgtga gaaagcaacc
    23281 gaacaccacg aagacaaggc tgtggccggg aatgcccgtt ggcatgcctt cgggcgtgta
    23341 cttgctgaag cgcggaccca gtatccagac catggaaagc gcggtcaatc cgcccaccac
    23401 ctggatcgta gctgcgcctc cgcaatcgag gaagccttgt ccaaggccgt agttgacgcc
    23461 cagttgcgca agccagcctc cgccccaaac ccaatgcgcg aatatcggat aggtgcagcc
    23521 ggcaaggatt gccgtggatg cgcaaatagc tcccaaccgc cagcgatcca tcgccgcgcc
    23581 caaaggaatc aaagcggcga gagcgactcc gaacaactgc agcaatgccg cgagcgatgc
    23641 gggcgagcca tcgagttcga tgccacgaaa aaagaagggt tccgcggcaa tccagttcca
    23701 gggttttccg cgcagcagcc atacgtgggc tggtccgccg gcaaaaccgt cccacgaaaa
    23761 accgcagacg acatagacga tggcagcgac cgaaattgcg cacagcgatg ccagcatgac
    23821 gtgggccgca ctgcgcgagc ggccgaggcc gctattcatt acggcgaggc cggccatggc
    23881 cagaggaatc agaagaattg agacgaggca gaccgccccg gctgaggtgt cggaaaacgc
    23941 gggtgcggaa gcgggaatca tcgagcttcc tcgctctggg gtaaatggga gcgaaacagc
    24001 aggagcagtc ccatagattc cacggcgata cccgccagga caaatgcaac cagcatctgc
    24061 gttgaccgga gaaggtccag cgcagacagg acgatccccc agcccgccag cagaaataga
    24121 atgcccgcaa gtctcatgat tgattgaaca acggaacgaa tttcgtgaca ggctgacacg
    24181 cagacgcccg cgttactctg cgagcgacag ctggccaaaa attaagctta tggatcgcac
    24241 gcaggtgcgt ccaatcgatt ttttcgatgc cagccaaaag cagaaccatg tcagactcgg
    24301 gaagttgttg ctactataaa tccagagacg ctgtaccacc cgaaaatttc atgaaagata
    24361 ttctgaacca gatcgtcgcc acccgtaccg gcacgtttgt cgtgctgctg ctcgctgcct
    24421 tcctcgaagc ctatggagat tcgtgcttcc agaccggact ctaccggtcg tcaggagcgg
    24481 cgcgctatgc cgcctttgct ggcggcgcga tcacgctgtc gctctacggg ttggtcgtga
    24541 atacccctcc gtgggagttc ggcaagttac tggggttgta tgtcgtgctc ttctttatcg
    24601 ttgcccaggt gctggcgaag gtgagattca accaggctcc gactcctcca atcctggtgg
    24661 gagggtcgct gatcctgctg ggcgggatga tcatttcgtt ctggcggaaa tgatcattcg
    24721 gtagtcgcta tacctgaagg acatctcctt cgcgggcaac catctggtac agcaccgggt
    24781 tgacgaagaa tccgatgaac aaccgtgaaa ttagtcctgc cacgatgacg atggcaaatg
    24841 gtttctgggt gtcggagccg attcccgtgg cgagtgcagc gggaagcagc cccaggcagg
    24901 cgacgagcgc cgtcatcatg atgggacgaa gtcgcaggag cgacgcttca cgagttgccg
    24961 tgcggatgtc catgccttcc aggcgaagct tgttgatgta ggaaaccagg atgaccgctg
    25021 tttccaccga aacgcccaga agcgccagta atccgagaac tgacgagacg ctgaacggag
    25081 tatgcgtcag cttcagcgct atcaatgcgc cgacaggctc agtgagaatg acgcccagcg
    25141 caatcgttac cgggaacttg aagtttccgt agagtgcaaa caggatcatg aagataagga
    25201 cgaccgcgag tggtccgatc agcaccagtt gcgctttcgc ttccagaaac tggctgtatt
    25261 caccgcccca attcagtgtg tagccttggg gcagagcgac agcttgatcg accgccttct
    25321 ggccttcatg cacggcgcgc tccaggtcgc gtccttcaat gctgtactgc actccgatgt
    25381 agcgggagtt gttctcgcgg tagataaacg atgcaccgtt tcccaccttg atttcagcca
    25441 gttgggttag tggaatctgt tttccatccg gagtgccgac cagcagctcg ccgatgtcgt
    25501 gggcgctgct gcggaactga ggttgcatgc gaaccacaag gtcaaacaac ttctcaccct
    25561 ggatgacctg ggtcaccgcc tgtccgccta ccgccgccga aatcacggct tccacgtcgg
    25621 cgacattgat tccgtagcgc gcaatcttgt cacgatccac gtcgatcagc aaacttggtt
    25681 ggccaagttc gcgcaccacg gtcaagtcag tgaaacccgg aattttggcc agggtgtttt
    25741 tgatctgaat cgctttgtcc tggagtacgc ctaaatcctc gccatagatt ttgacagcca
    25801 gtgaactctt caagccggtc agcgcctcgt ccacggcatc ttcggcgggc tgcgtgtagt
    25861 taaaaacgat tccaggaaac gcgctcagct tctggttgat agcttcgatc agcttggcct
    25921 tggtattgag gtcaccactc caagccgcgt ctttgtaggg tttcaggccg acatagaatt
    25981 cgcaattgaa aaagccggtg ggatcggtgc catcgtccgg gcgccccagt tctgaggcga
    26041 ccaccgtgac ttgtggatag gacgtcagga tggcgcgtat ctgaggagcg atgcggctcg
    26101 cctcttcgaa cgaaattgtg taaggcatgg tggcgcgaac ccacagcgcg ccctcgtcga
    26161 ggtgcggcat gaattcgccg ccgataaagg gaacgagcag cagggttgcg ccgaagataa
    26221 ggaccgcgat cagcatggta agtttgggcc gcgctaacgc ccaatccaac tgcgtagcgt
    26281 acctgtcctt catccattcg tatgcacggt ttgatttttc acgcacgccg ttcttgaacc
    26341 agtacgaggc caggacgggg accagggtga gcgtgaggat cagcgcgcca accaatgcga
    26401 acgacatggt ctctgccatg ggatgaaaca gcttcgccga aggtccggtg agcgcgtaga
    26461 tagggagata tccggcgagg atcacggcaa ccgagtagaa gattgggcga tccacgtctt
    26521 tcgcggccgc cagtatcact tcattcagct tgtaggtctg cccttctcgc agcgcgagtt
    26581 cgcgatagat gttttccacc atgacgaccg taccgtcgat gatgattccg aaatcgatgg
    26641 cgccgatgga gagcaggttg gcggcgccat cgtgggcatg taaggcaatg aacgcgaaca
    26701 agagagaaag cggaatagtg agcgcaacga tgatggctgc gcgaaaactg accaggaaga
    26761 agatcagcac gaccagcact agtatcattc cgcggagcaa gttgccttcc accgtgtcgg
    26821 tggtcagctt tacgagctca ctgcggtcat aatatggatg gattttgaca tccggcggta
    26881 agacgttccg attcagttcc tcggtcttgg cttcgacgcc tttaaggaca ttctgcgttt
    26941 gctccccgcg gagcatgaga atgacgcctt caacggcatc atcgttcttc tgatagccga
    27001 attgaccgag tcgaggagcg tagccaatca cgacgtcgcc aatgtccttg atccgaatgg
    27061 gcacgccgtt gctactgccg acgacgatgt tcccgatgtc gctcgtatta cgcaccaacc
    27121 cgataccgcg cacgtagtaa aactgtccac cttgagaata gaatccgccg cccgcattgg
    27181 agttgttggc gcccaaagct gtcatcactt gcggaacggt caggtgatat ccataaatcc
    27241 tggcgggatc gagcagcact tgatattgca tggtggtgcc gccgaaaccg gagtcatcgg
    27301 cgacaccacg gacggaacgg tacgcccgtt cgaccaccca atcctcgatg gtcttcagtt
    27361 cctgcggact gcgatcggga ctctccagca catagcgata caccagtccg ctggggctaa
    27421 ataacggcgc catgttcggc gtcactcccg caggcagcgc ggcttgagcg attcgctcga
    27481 atacaatctg ccgcgcaaaa tagtcgttgg tattgtcttc aaaggtcagg atcacatcgg
    27541 acaggccgta aagcgagatg gaccgcatca cgaccatgtg cggaacgccg ttcatctcta
    27601 gttcgatggg aagcgtgacc aggcgctcga tttcctcggc agcatggccc ggccattgcg
    27661 tgatgagttc gacttgcggt ggcgaaagat ctggataggc gtccaccggc atgcgccgga
    27721 acgaaatgat ccctgcgatc atgatgaagc ccgtcagcat caacaccaag aagcgctgac
    27781 gaagcgacgc ttgtacgatt cgatggatca ttcaggtgtg gacggcacgg tttttatcat
    27841 gatttgttgg tcgagttcgg ttgtttcggt ttaacgctgc agcgagttcg caaactgcag
    27901 aaacaagctg ccatctccga tgacgcgatt accggggttt aatccggtca gaatctgggt
    27961 ttgtcccttg atgctgtctc ctatagtgac ggtgcgtctt ccaaattgat tgccagccga
    28021 ttcgacgtac acaaaaggct gattttcact atcccgcagc acagcggaat ccggtacggc
    28081 gatggcgttc tggatcgtgc ccgcgtccac cgtcgccact acgtacatgt ctttcttcag
    28141 tttcagtcct gggttgctgg tttcaatgcg cgcctgcagc gttcgagtgt tcgggtcgag
    28201 ggacgcagcc acgtaggaaa tacgcccacg aaacacgtcg ggataggact cggtctggat
    28261 cgtgacctga tcgccgacgt gcacgtaggg cagatccttc tgataaatat tgaccagcac
    28321 ccaaactgtg ctggtgtctg aaatcacgaa agtctgggtg gcgcccgtct gaagcagttg
    28381 tccagccgcc acgtcctgct cgaccacttc accactgatg ggggccttta ccagcgcctc
    28441 gaacgaaggt ggcgccttta ccagtgtttc gggattggtg attcccaaga ccttcaaggc
    28501 ggcttccgcc gatgtcaaat ccccttgtgc ttgcgtctgg gcggattcag cctgctcgag
    28561 attctgttcg gcgagggcat gatgctgata caagtcctgc gcgcgcgcgt aagccttttg
    28621 tgctagagag tacgcgtcct tggccttcag atagttggtg cgcagctgcg aatagtcggg
    28681 actcgccacg tacagcaggg gctcgccccg ttgtacatgc tggccgggga ccactacgat
    28741 gcgactcacc ggaccgctga cctgcgtgat caccggagtg gtgtggaagc tgttgtaggc
    28801 caccgctcca ctcagccgga ggctgcgcgt caggttggcg ggctggaccg tgagcacctg
    28861 cacatgggcc atctgctccg aagggatggt gaacagttca gcattgtttg agttgttggc
    28921 atggttcttg gccgcgttat ccggtccggc gctgctgcag ccggcgaacg ccagcacaat
    28981 cgcgaataga accgttagcg cgacccgtcg tcccatggga tggttgtata tggtcacgga
    29041 aggttccttg ttcccacagc ttctttgagc tgctcgagtg cggtcatgta tgtcgaaaga
    29101 gcctggcgat aggccagttg gatcgcccga ttgctgcgtt cggcgtcgag aaaatccaac
    29161 aggcctgtgg cgccgcgttt gtaagcatac tcactgatgt cgcgcgagtc ctgcgcctgc
    29221 ttcaagtatc cggaagtata gagttgtacc acttcttcgc tgctgcgaag cgattcatag
    29281 gcatcgctga catcagacag cacggtgtcg ctcgccgagg actgcatctc ctgggcctgt
    29341 gttagtgcat agcccgtacg cgcaatttcg ccttgattgc ggttgaagat gggcaacccg
    29401 aaattcacaa agaacgaagc gctgttctgg tcggcggtgt gggtgtagtc aacgttagcg
    29461 ctcacgtcct gtttggcgtt cgccttcgcc aactggatct ggctctgcgc agcggtcacc
    29521 cctagttcgg ccgcacgaaa atcgggacgc tgctgcagcg cctcggcctg caagtcctcc
    29581 agcttcgcct ggaccggctc atacgataga tcgccaacca cgtcgtaatt ggatgggacc
    29641 gcatcgtagc ccagcaattc ccgtaatgcg accagcgcct gcaccctcgc caagcgggcg
    29701 gaagaaacat cggtttgaaa ctgcagcagt tgcagcttga tcttgaggta atcgccttcg
    29761 ctgatgtatc ccgacttgta ttgcgtctgc gcgatatcga ccgtgtcctc aaagctctta
    29821 agatcgagca gcgcaaaccg cagagtggat tcggccagca acacgccgac gaactgctgc
    29881 gccacattga aggtgagcgt gcgttccgca tccgaaactt gggcaatttg cacagccgtc
    29941 tggtcgcgag cggcttgcaa gcggtgctgt cgttttcggc ctcgctcaaa cagatatcca
    30001 attccgacat cgaattgctg gatttcgctc agagcgtcag ccgaaaagtc tgatggctga
    30061 aaaatcggta aaaattggga atcaaagttc agcgtcgggt tgggacgcag gttggcagtg
    30121 atttcctgcg cctggttctg cagaatgaga gtgcgcgtcg ccttcagcga atgattgtga
    30181 gcgagcgcca gattgattgc ctgatccaaa gtgatcagcg ttggattttg tcccaggagc
    30241 gcgggcacca gcaacagcag acaagtcaaa gctgccaggc cgatcttcct gaagtgtgat
    30301 ggacgcataa gatcagatct aaaatgcaag accccgaggt gaatagtcag ggccatcaaa
    30361 tgcactgccc cagactctca attccgtgcc gttagcctgg gtcaatcgtg cccgtggctg
    30421 gcaagggcct attgggcaga acgtaaaact atctaaacag agaacacatt ctaaatgaag
    30481 aagttgcagc aggcgggggc gccggatagc gtggtgggtc ttcgcggagg aacccttact
    30541 ggcgcaccag catggcgagt cgcgggaaga caaaacgccc ggccgcatcg tcaatcacgt
    30601 tgacctggca ggtgtagaag cccggcttca gctccgatag cggcacatca agctggaaca
    30661 ccgcggcctt acggtttggc accgtgagct ggtgcactct taccagcgga gtttcatagg
    30721 ctttggtgtc gccttggaaa aacgcgacat tgctcatcac ccgcacgccg ggcttagagt
    30781 tcttgtccgt cggggcgggg ctgatttcgg gcgcggtttc tttcgccgga tcgtacacct
    30841 cgtaataaat atagagatgc tggttggccg agaacacccg ggtaacgctg ggcacaattt
    30901 cttgtccatt ctgcaccagt gggttgccag tatctctctt gggtgcagtc tgcaactggc
    30961 tggccatcac gatcgagctc atcttaaggg gcgaagactt gagatcgggg atggtgaagt
    31021 cagtctcgaa cgatcccatg cgtccggctt cgttctcccg aacgacaacc ttcaggtgat
    31081 acttgccggg aatcagcgtc aggccggtat cgtactgcac attcttgcgc tgcacttcat
    31141 tggacgcatt caccaccagc ttcacggttt cgcggatctc gctgactgga aatttcttct
    31201 catcggtaac cagcgcgagg atgtcgagtg tggccttgtc ctggtcgcga ctgcggctga
    31261 acggaatctc agaacctggc acaattagcg acaatggaac atagaaccgg ttatcggata
    31321 ctcggaaata ggctgtcgcc aggtaaaccg gcaagtcggt actcggtagt tctgatgcca
    31381 gctcttctcg cagttgccgc tcgcgatcgt ctttgttgga gtgccggaag tctgcctcag
    31441 catagtatcc gcgacgggcg tcgaccttga cgccgggacg gttcacgcgc actgtgatgt
    31501 gccgatactt gccatcgcga gcggtgttgg tactgtgata gcccagcagg tagtacatgg
    31561 acgtgtcgtc ctgcacgccc ttgaagacgc ggccaaaatc gttagaatcg aggaacgctt
    31621 ttccgccggt gtcggcagcc agtgtaacta gcgtttcctg cgtctggaaa ttcgaattca
    31681 acgcgttcaa ctgcgcctgt cctgaatacg ccgaggttcc gcgcaggctg gcgttctgtg
    31741 cctctccgcc aggcggcagc gcctgcagtc cgcgaatgtc caaggtgtag attgacaggt
    31801 tggctagaac ggctgaattg atggcggcac gcaactcgga ttggttttcg atgcccgtgc
    31861 gctgcattcc actggagaag tagatcaacg attttttctg gggaacgttc gccacctgat
    31921 cggcaatggc gcgcaaggcc tgcaatcgcc ggtcggtgtt gaagatgttg tactcggtat
    31981 cgtcaaccgt gaaggactgt ccggagtcag gcgttccttc ggtagttccg gtcgagccca
    32041 gttcgaatcc ggcgttcgct cccacgttga acgactgcag tacggccttc aatttttcgc
    32101 gatccgaagt gaagtcctgg ttgacggtga tggtatttcc cagcgagacc accgaaacga
    32161 gatcggcagg ctgcatttgc ttagcgaggt agtcctgcgc ggcgttagaa gagcgctcga
    32221 tttcgtcggg ttgcatggcc gacaaatcga agaacaggat gatcaacctg cgatctttaa
    32281 tcgccgactc ctgggcgctg gctgcaggag ctgttgcact tgtcttcgtc tttgtcaccg
    32341 gcgtgctgag caggttcgcc tgctgcatcg gaacctgttg cacattttgt gtgttctcga
    32401 tatcgaaggt cgccaccttt tgcggcttgt tgtcttcgag gacgatgaag tcttccggtt
    32461 tcaagtcacg caccggattg ccattcttgt cacgcaccgt gacgttcacc agcaccagct
    32521 ccgtctgcgc cttgaaggtg tagccggtct gagactgctg cgccagtagc gcggtcggaa
    32581 gaagtaaccc aaccatcgca accagtgcgg tcagcttgcg tctcaactcg atgaatggcc
    32641 gcaacatggg aattctcaaa accggaacct cgctgtcagc aacaccgccc gcatggcact
    32701 gaccgaaatc acgcggccga acgttggaga gttcagcacc gtgtcgatcg acgagtagat
    32761 cgggtggttg aagacgttgg ttgacgacag ccgcagttcc aggctgcgcg attctttcaa
    32821 cgggatgagc ttggtcattg ccatgtcgaa cagtacctgg cctgggccga tgatgctgtt
    32881 gcgacgggcg tccccatact ggcctgtcgg cgggataacg aacgccgcgg tgttaaacca
    32941 ctcaccgatg ctggggttcg gaacgctaac tggaagccca gtaacgtcgg cccgcaacgt
    33001 gccattcacg ccgctgttga cgttggagta cgagccaagt atgcgcgggg tgaacggcag
    33061 gccggacgcg atggtccaat cgccgctcca ttgccatccg ccgagaagat cctgcgccaa
    33121 tcccgcggtc gaaagccaac gtctgccctt ccccaacggc aactcccata ggtagtcact
    33181 ggtaaagcgg aactcctgat tgaagctcga caacccccgt tcagcagaga ggtcgaaagc
    33241 attttgcgcc acaacggtga cgccggtgat cttgccgttg gcgctgacga tcgacgatcc
    33301 cgccccgatg gtcgatgcat cgtcgagcga cttcgaccag gtaaacgttc cgcccaggga
    33361 aaccccttga ctcagatgct tgcgcacgcg caccgagccg gcgttggccg tcgagtcgcc
    33421 gtttgacgac tcgtaatagt acggctgaac gccgggtacg aggactcccg tcgccgtcga
    33481 attcggcgca ctgaggatgt cgagccgcgt acctttggtt ccggtgtagt cgaggttcaa
    33541 aagcaatgtg cgagtgattt cctgctggat gtcgaggttc cagatctgca catagccaag
    33601 tccgtaattt tgctggatcc cgtaattgtt ggtgagcgag gtgggcgtgg ggaagccatt
    33661 ctgcaaagtc agaatgtttg acggggaagc gacgttggtc tgggtattgg agaacggcgg
    33721 ctggaacgcc atgttctgca cgatgttctg atacgcggca gtgttgtagt tgatcccata
    33781 tccagcgcgc acgacggtgt tcttcgccgc cttccatgcc acgccgacgc gcggcgcgaa
    33841 attgttgcgg tccggattca ccagggtgat gggatacacc gatcccgtta ccgggcccac
    33901 ttgtccagga agcaccggag cgacttcggt aaagctcgaa ttggtgttga ggttgacgat
    33961 gcggtcattc gactcgctca atggcgacac gtattcatag cgcagaccga cgttgaaggt
    34021 cagattgccg cgcgccttcc attcgtcctg cacaaacatg tcccaggagt tcccgcgaaa
    34081 gtaatagcgg tcgtatccat attgcaccga ggtctgctgc gccgtgccca gcaggaaatc
    34141 cgcgaaatcg tatcctgtcc ccggcacggc aacgccgttt acgaattgcg atgtcagcgc
    34201 gccggtgaac acaaagctgc cgcgcggatt gctgtcggtc tgagtgttga gttggatgcg
    34261 ccggaagtct cctccccaac gcacggtgtg tttgccgtgc gaccagatca tattgtcggt
    34321 gaaggtccag gtctggttgc gatcattcac cggattggtg tcagtgatgc cgccgaaatg
    34381 agtaaacgac agattgggca gcccccatgc gaaggggttc tgggaaacac cagtgatgcc
    34441 gagctgtccg gttatgtcag tgttgaaagc gtacaggttg ttcgtgtaga tagtgctgcg
    34501 gttgaagtcg aaacgaaagt tgttaaccaa cttgccgaag ctacgaacgt aaccgatggg
    34561 gatgtcataa ccgttggtgg tggtgtgccc gccgaccgtc ggaaatgagt tgatcaacgt
    34621 ctggtcgaca ttgtgatagt gaaacccgat actcagatta ttttgcggtc cacgtgcccg
    34681 tcctcgcccc ggcgaaaccg acgaccctcc gaaagcctgg ttcagccgga agttgaggtc
    34741 agtagtgtta tttgtggctg ccgtgaggta ctggaagttc tgggtggtcc ccggtaggtt
    34801 gggcgtgggt atgtatttca ggagccccag tgacgccgag ctgatcatgt tctgcgggac
    34861 ggtcgcgttg gtaaacggca atcccgtcgc cggatcgaag atctgaacag gcttgccgtt
    34921 gagcagagtc tgcgaaaaat cgccgttgcg ttccagggcg gtcggtactg tcgtcaggaa
    34981 gttgtagggc gtatcaccat aggtgccgtt gtaattgaag aagtaaaacg tcttcgagcc
    35041 gccgtgatag atatgcggaa tattcagcgg accgccgacg gatgctccga agtgctgctg
    35101 caggtacccg ggttgttccg acggttggcc ggacagagca tacggcgttg cgtccagggt
    35161 cgaggtattc gccgagtagt acaacgtgcc gtggggctgg ttaaagttga agccgccacg
    35221 gcggccaccc atcccgccgc ctccaagagg accgaaccct ccgccaccag gaccgccccc
    35281 cggtccacct cctggtccga atccaccacc gggaccaccc ggtccgccct gttggttgcg
    35341 aaattcctga gcgcgtgtcc gcatctcgtc gctgttcatg tcgaagatgt ttccggatgc
    35401 tgacgaccca gagacggcaa ccgactcggt cgccatggaa ggtggaatgc ccggaaccgg
    35461 catgcccgca gtcgcaactg aatcgctgct tcctgaactg ttggaattgt tgcccgcttc
    35521 cgccgccatc acggaaagcg tctggaaacc gcgattgccc gcgccgcctc cacgcgcata
    35581 tgcgctcggg cccgcgttct gagctcggga aagcagagta atctgcaagt cgagccgggg
    35641 atgcaagttc gtcgtgctca cattcacgcg ttcagtcgca actgcaaagc ccgccatctg
    35701 gacgcggacg gcatattcac cagggcttgt cagcgcgagt gtgtagctac cgtcagcctg
    35761 cgtccagcca acggttttct gtccggtggc ggaattggtc gcgctcactg tcactcccgg
    35821 caccagcgtg tttccggatt taactctgcc tgtgagagtg ccggcaggcg cggggggtgc
    35881 agatggggca ggcgactggc ttgtcttcgt gatcgcctcc ggattcgccg agccggtctg
    35941 ggctgtgttg gtcgaactgt ccgacggtgg aggggcgggc gtcgcagggt tctgtgcgac
    36001 gaggctgaca cagcagagta ctgaaaacag aagtccgatc atccgccatt gacggcgaac
    36061 ttgtgatgcc atgattcggt cagtcgcggc ggacggctcg gtatcgctga gcccgacaag
    36121 acactcgtga agagaagata gagagaccga tcggagtgct cgaattttag acgtgtgagt
    36181 aatggcgacg ggtacctcag aggctcaatg caatccgcgg gcaatccgac ctgagctatc
    36241 agctttaacc ctgcatcctt agtagatgtt cgctgaaatg gcagcgaggg ttccggagat
    36301 ttgccgtgcg tctgactcga tgccagccac caaaacaggc cactggatcc gatgttacaa
    36361 gagacgcagc caccctgccg tggtcgagcc tctgggctat cccgggaacg gtgcgcccgc
    36421 ccgcacgacg cggatatcat gatcggtaaa tttgtggtgg acgtcgggaa agtcgtactt
    36481 tggcatgtga cacccggcgc agtctttagc gttcaccgga catgcgtgcc ccggtcgatc
    36541 agcagcggac ttggattgtg cagccgtgac atggcacgcc aggcatttct gatcgtagga
    36601 cgatacttcg tgcacaatcg gctggtgagg atcgtggcat gcggtgcact ggatgcgtga
    36661 atcattcctc cagcaacggc tgttctgcag gcgataggcg ggaaatcgga ccgtcggaag
    36721 tccaaggcca ccgtcaagct gaatgtccat ggacgttgcg tggcaggcgc cacagaagtc
    36781 cacctggtcg actggcctga ggcgagcggg gttcatgatg agcgccgtgc cctgcggcaa
    36841 gccagcctgc atggcgacgg tgtgatcctt tcccggaccg tggcaagctt cgcaactgac
    36901 ccccggaata acgttggcga tcgggccgct cgccggcaca tcagcggcat gacaactgaa
    36961 gcagcgccgg gcttccacca tgttcacggt gcgcccgcca gcccgcttca tggcatcgga
    37021 gtgttgttcc ccgatcgtag aagctacgcg caccgatggt tgcgcaggag tcagatcgaa
    37081 gccgtcgatg gactcgaagt agctgaagtg gctttcgttg aaatggtctt cttcgggagt
    37141 tgaatagacc tggctgatct tgcccgagcc gaatgcccaa gtcaatggct tagtgatagg
    37201 agaagtgctg gaaaccactt ggaaagcagg gccctctggg ccacgaaaaa tccggtacac
    37261 aaaaccgtcc accgtcacct gctttccata acggctggtc agaaaggggg actgctcggc
    37321 gggcatcagg gtctgggcca tctccgacgt cgcctgcgac ttcacgatgg cagcgtgaca
    37381 ggcggcacag acctcattcc ccacgaattt atcgggcgcg ctagagagtt tagtcggcca
    37441 ccacacttgc tgtcgcagac gctccggcgt agtcatctgg tcgaccatgt tgctttgcga
    37501 cgaggctgcc accacagtca cgagcacagt gccgaaaacc aggacgagac gcataccggg
    37561 catcattgtt ctcacgccaa cgttggtgat tcgcctacac agcacgagga ttcactgcgc
    37621 cgctgtggtt gcgccggtga cgggttccgc gaacctggaa tcttctattg gcaggtttcg
    37681 ctgaatttcc tgcagctgca ccaggaaccg gccctggggc tgcgagatcg tacgttgata
    37741 gggaaccttg gtccccgcca caacgcgata gtcggaaaag tcgatctgcg tcggcgtgcg
    37801 gcccaagggt gattcgacgt aacgaaccat gcgaaccagg agcccagact ctgcatcgaa
    37861 atacagctcg actggcggca gtcctgggct gatagcaaaa ataacgttga ccttgtgacc
    37921 gtcaatctct gtgtcagggc gggactcgat ggtggggaag agagacttga tatctgccgc
    37981 gaactggaga tccgcgtcca ggtgggctgc cagaagatcg ctggaattca tttcgtgcac
    38041 cggccttccg ggaaccgaga tccatcctga aggcccgctc actacctcga ggctgtcacc
    38101 ggttggaaga tgcatcgtca tgcagcgcat tccggggctc ttagtgtaaa tctccacggg
    38161 aaactgcacc ccgttgccga gattgactac acccttctcc ccctgtgttt tggctgcctg
    38221 gagggcactg tcacccccga tagcctgcaa gtatttcgtg aggacttgat ctacggaacg
    38281 cgatgccgaa attgctgccg gggtcctctt gggagccgtc gtttcgcttg cgaccgcagg
    38341 ggtattcgct gggcgtaaat ttccgcgatg gcaggagtag caagtgacgt cgcgttctcc
    38401 ggcaaagtta ttctggttta aagccagcac catttggatc atcttccgag ctgtctgttt
    38461 tggcttcttg tcatcgctgt cgaatgcgtg ttcgacgtga cagtgctcgc agcgcacgcc
    38521 gagcgactgc gtgatgaact gcatggcagg aatgagttga tcggcgggag tacctttcag
    38581 gacctggata tttttgtaga cttgctctgc cggtttggga ctgatgactg tttgcgactg
    38641 tgcccatgcg cacggaagcg gtgcgaacgc aacgcagcct gcaaccatca gaacgcgacg
    38701 acgaaacatc tagaagccca accttacttt ttgcagaaac actcgcattc cggcagattg
    38761 cgcgcttatc ccccaatgct agtccgccac ggccaccgct ttcccggaga ttacgagtcc
    38821 gtaaaggaag cccatcggac agcaaagtcc gtctagcgcc cgatgatgct agccaacagt
    38881 ggacttagat attaaacata ttcggaagat tgcaggccct ggttccacta attttgtaaa
    38941 gaaaactgcg ggaaacaaat tcccgacaat cgggtttcag cccacgtagt tagattgaca
    39001 ctcgattcgc aatggaatga atgtggcacg cgtcctacga aaactcggaa gtctctggat
    39061 gacgatgttg acatcgaacg cgcctctcac ggcggcctcg gcatacgtgg ctgcctccat
    39121 cctgcatcga gtgtccagga gcgttggaca atgaagaaga accttttacg agcagccgtg
    39181 attttcacgt tattggcggg cagcaccgcg atctatgctc aggcgcctca ggaacagagc
    39241 ggtggccagt ggggccacgg ccaggggcaa cccatgacgg tcgatcagcg gttgcagaac
    39301 atgaccaagc agttaaacct gagcgacgcc cagcagcagc agatcaaacc catcctggag
    39361 aacgaagcca aacagatgca gagcgtgcgt gaggacacgt cgcttgcaca gcaggatcgc
    39421 aggaccaaga tgatgcagat ccggcaggag acctccagcc agatcaaacc catcttgaat
    39481 gccgatcagc agaaacaata tgacgagacg atgatgcgga tgaaccgcgg acgcggcgcg
    39541 ggcgggccgg ggcagccgct gccgccgctg ccgcccagcg caagccagcc accgcagcaa
    39601 tagccaacct gcaaggcaga tcgaagactc ataaaccacg atcggcgtgc gggcgaatgc
    39661 cgtcgatcgt ggtttatgtg cggaggacga gaaggcttca accagcaaac gtccgtctgg
    39721 gggaattctt gcgtgccgct atcgcacgaa aacgacaggc ttttcaggat cgcgactctc
    39781 gagcaatgat gattggaatc tcgagcttct tgcgtccgtg gttttgacct accgcgttcg
    39841 aattggccgc gacgttgccc agcaccaagt ggcacacttc tttcacatag ctctcgctga
    39901 gttcgtcatg acggatgaga aagcgcatgt agatcgcgcc atagagcacg tccagcacaa
    39961 tatccaagtc caatgtctta ggcagttcgc cccggtcgat gccgcgttgc agagtcttgt
    40021 aggcctcctg tcggcgaggc cgcaggaaac gctcgcggaa tgcctccagc agtttgggat
    40081 cggcctgccc ggcgcccaat agctccgcga caattcgtcc ccggcgagta cgcagaattc
    40141 ccaggaattg gttcatctgc aggctcatgt cgcggtatac ggacccggtg tcgggaaagt
    40201 gaagcttggt ttcggtgctg gaagcgaagg cgtccaccac cagagcgccc ttgtctggcc
    40261 accagcgata cacggtggtc ttgcccaccc tggcgtcagc ggcgatcgct tcaatcgaaa
    40321 ggtcggggaa gccggagcgt tgcagtagct tcagcgtact gcgaagaatc gcgtggcggg
    40381 catgttcgct ccgcggccgg ccgggctgac gtttggcccc gttgtcgcgc tccagccttt
    40441 gaccagaagt cgattgccgc atgagccaag atagcatata ttgaatccac tacgtaccgt
    40501 atcgaatcgt aaatttgtac cgtagctgcg ccggcgttga ccgccgtatc atcaccccgg
    40561 actcgatggg agttttgccc gaccgatgtg gattgttgca cttgcgctga ggcgtcccta
    40621 caccttcgtc gtcatggcgc tggtgctgct catcctcacc cccatcgtca ttttgcgcac
    40681 gcccgtcgat atcttcccag acatcaacat tcccgtaatc agcgtggtgt ggaattacgg
    40741 tggtttcgcg cccagcgaga tggccgaccg catcgtcact aactccgaac gcggcctgac
    40801 catcacggtc aacgatatcg agcacatcga gtcgcagtcg gtgaacggcg tgggcgtgat
    40861 caagattttc ttccgtcccc aagccaatat ccagttggca ctttcgcagg tgacggcgat
    40921 ggtgcagacc acggtgcgcg gattgcctcc cggaaccact ccgccgctgg tcattacgta
    40981 ttccgcgtcg tcgaccccga tcgtgcaact gggcctcagc agcaagacgc taccggaaca
    41041 gcaactgttc gaccttggcc agaactttct tcgctcgcaa ctggcgaccg ggcacggagc
    41101 cgccacgccc tatccctacg gcggcaagat tcggcaggtg caagtcgatc tcgacatccc
    41161 gcggctgcaa gccaacggat tgtctcccaa tgacattgtt agcgccatca attcgcagaa
    41221 cctgattaca ccgtcgggct cggccaaaat tggctccctc gaatatcagg tggaaatgaa
    41281 cagctcgccg caaaccatcg cggagctgaa cgaccttccg gtaaaaaccg tgaacggctc
    41341 gaccatctac ctgcgcgatg tcgcgcacgt gcgtgacgga ttcgcgccgc aaaccaacat
    41401 cgtgcgccag gatggggttc gtggcactgt gatggcgatc tacaagatcg gcagcgcctc
    41461 gacgctgagc atcgtcgccg ctgtgaaggg cgtgttgccg attgccgcac agtcactgcc
    41521 taaagacatc actatcaagc cgctgttcga ccagtcgctg ttcgtgcggg cttccatcaa
    41581 cggcgtgata cgcgaagcct tgattgccgc tgcgctgacg gcgatgatga ttctcttgtt
    41641 cctgggcgac tggcgtccca ccatcgtgat ctccatctcg atcccgctct cgatcttcgt
    41701 gtcggtgatc gtgctgagcg cgctgggaga gaccatcaac atcatgacgc tcggaggcct
    41761 ggccctcgcc gtcggcatac tcgtcgacga cgcgaccgtg gaaatcgaga acatcgagcg
    41821 caaccttgcc cagggcaagg aaatgaagca ggcaattctc gacggcgcgg cgcagattgc
    41881 agttccggcg ttcgtctcca cgctgtgcat ctgcatcgtg tttgtgccga tgttctttct
    41941 caccggcgta gcgcgttatc ttttcgtgcc gctggccgaa gccgtcgttt tcgccatgct
    42001 ggcctcgtat tttctgtcgc ggacgctgat cccgacgttg gtgatgtaca tcatgcgcgg
    42061 ccatgagcac agggccgatg gaccgaagac gatcttcgga cgctaccagc gcgggtttga
    42121 agccagtttc gagaagttcc gccgcggtta ttaccagctt ctcgaaacca cgctcggaca
    42181 cagcaaggtt ttcgctgcct gcttcctcgc attttgcgtt ttatcgatgg gactggtgtt
    42241 cttcctggga gaagacttct ttccgtcagt ggatgccggc ctgatgcggc tgcacgtgcg
    42301 cgctcgcgcc ggcatgaggg tggaagagac cgcccggctc tgcgacgaga tcgagaagta
    42361 cctgcaccag cagattccca aagacgagtt ggtaacgatc ctcgacaata ttgggctgcc
    42421 gtattcgggc ttgaatcttt cctatagtaa ttccggcgtg atcggcacca gcgacgcgga
    42481 gaacctggta ggactgaacc ccgaacatca tcaccccacg ggggaatata tccgcacgct
    42541 gcgcgaagaa ttgcctcgtc gcttccccgg cgtggagttc ttcttccagc ccgccgatat
    42601 cgttacccag attctgaact tcggattgcc ggctccgatt gatattcaag tcaccggcgc
    42661 cgacatgcag ggcaactatt tcatcgcgca gaagattgca aaccgggtga aacagattcc
    42721 cggcaccgct gacgtgcacg tgcagcagat gttgacattg cc
//
LOCUS       DASRKK010000416        19111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_423158, whole genome shotgun sequence.
ACCESSION   DASRKK010000416 DASRKK010000000
VERSION     DASRKK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19111
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423158"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFC15_09605"
     CDS             <1..715
                     /locus_tag="VFC15_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM10452.1"
                     /translation="ARNSAIRQAKGQWLAFLDQDDVWLNGKLQQQMALADDHAGIIYG
                     RAIQFYPSGEQRDYDYAHEFQPLPEGAIFSQLFTDACFIAMSSAVLRRSAVEEVGPVP
                     ETIQSVPDYYLYVAVARRYPARAVQEAVCRYRMHAGSMSRTNRQRLHEEPLLIIDQWA
                     DCLDPGIVAHRRMTYSTALALEEMRSRKSARKGLVRMLTDGSVIWLMSRPLVRAFRAV
                     RRRLCRPYWLRTDFASDRD"
     gene            complement(676..1716)
                     /locus_tag="VFC15_09610"
     CDS             complement(676..1716)
                     /locus_tag="VFC15_09610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM10453.1"
                     /translation="MTEPFLDLTVIIPSYNTRELLRNCLDSIYRYTDGIAFEVICVDG
                     NSPDGSADMVAEIFPDVILIRNAVNESYAKSANQGIRRSHGRYVCLLDSDTLMIENAL
                     EPLVRFMDEHPQAAVCGPKLLNPDGSIQHHIRSFASLAVFFLQTLNWHKLFPGSKLMN
                     RYYNTDFDYSKAQPVESIGTSAYVIRRSTWETYGLLDERFRWAMPDLAYNYTLHRKGC
                     KVFYTPCAEVVHFGGKTASQDVLRALREQRQGLIDFSEAYDYFGKSRVTKALVRFGVR
                     ARYYTKVLGYYLSSDKRVIKGPGAPKKEIAAQAALLLEPGARGAKGKGLEEATCASFS
                     QSRSDAKSVRSQ"
     gene            complement(1713..2273)
                     /locus_tag="VFC15_09615"
     CDS             complement(1713..2273)
                     /locus_tag="VFC15_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZM10454.1"
                     /translation="MARLKELLYSDLARQYELEGRSEIRPNFVRFLGRLLHFRFLPNV
                     LCRTSRAAMLAGIPILPKLLTYLNLVLFGLEVTPKCEIGAGIFFAHPVGSVIGAWQVG
                     SNVTIFQGVTLGSKRPDMKFDPALRPQIGNNVMLGTGCKVLGGIQIGDNVTVGANSVI
                     LNSVEPNQTVFGVPARAAPSSAGNPE"
     gene            complement(2337..3443)
                     /locus_tag="VFC15_09620"
     CDS             complement(2337..3443)
                     /locus_tag="VFC15_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZM10455.1"
                     /translation="MRVLYDHQVFSLQNAGGASRYFYELMNFLSTVPDVQTELLLGIS
                     GTVYPFRSLPPAKARVTQLPESLPPGMLRYVANEILGNCAAFVRGTMDIYHLTLFMRM
                     PMVRARRLVATHHDCTHERFPHLFPDVKKVLWARKKLFPEMDAIICVSESCRQDLLQF
                     YAVDPAKTRVIHHGLNPLPRSAEAAASLRQKLRRDYVLYVGMRVAFKNFAGLLKAFHE
                     TRLHDSFDLLVLGGGPLTVQEKTLIASLEMSDCLTALPIVSDELLAEAYAGAHLFVYP
                     SLNEGFGFPPLEAMSVGCPVLASQVSSIPEVCQDAPFYFDPMDQGSFNRALLQAVNDE
                     MARQLAVERGKEVVAKYSWDKCGRETLALYRECQ"
     gene            complement(4056..4232)
                     /locus_tag="VFC15_09625"
     CDS             complement(4056..4232)
                     /locus_tag="VFC15_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10456.1"
                     /translation="MTQDKFRDPSSISLVKTMLEDSILIPIVIVGFGRLLAAACSESG
                     NGVILVTSPQAEGA"
     gene            complement(4219..5730)
                     /locus_tag="VFC15_09630"
     CDS             complement(4219..5730)
                     /locus_tag="VFC15_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10457.1"
                     /translation="MTQTSSQSRAAFLVILIATLLHLSLIWLPGINLEWVFADGAKYF
                     ECYDPVLLKHYFLLQANTLGMPFLASVFHRLLPFLNQNAVLRVLSTSSFLLLGYSLLK
                     LCSLLERDYNPAVLLAVVFLNPLIWTFGGRGTADLFPAALTLFAITLFWQAKSPPLRL
                     AIAITAYGIAITLKYHAALLLPLVGLETLTRPGANIRKAFLRYSFIVALILVIPAAYI
                     ILAKQLYGFWFLPPVFRAIHGANLNLISIVMNMIGYAGYLSLLLVPYSLSAVWERVNT
                     TRKALITLVATIAIFIAGVYGVRLGEEMRFGPLDAYINPRIYSGTFLVFAAIFILLSK
                     DILLNTNTPSARRYILCMVLGIFIFVAILSLTRPAQRYLLFVLPLAYFFVMTKSRSGK
                     YITGTTILVYVILILFITLSQVATGAVAQNMVQQITARGLLDDTEAGDLMGNAGNRFP
                     DQVNGAAHKHYTVIAGNSPKAIVTVESHPAPLVLKVLSLVRYEDAPVTTHDSR"
     gene            complement(5795..6022)
                     /locus_tag="VFC15_09635"
     CDS             complement(5795..6022)
                     /locus_tag="VFC15_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10458.1"
                     /translation="MTNLETPSRAVVRFHNYISSRELQQQKLKYRVGLLGAKTEIPVY
                     TLAGSVVLDANSNVYGTTFCGAGVVFEIRPS"
     gene            complement(6264..7580)
                     /locus_tag="VFC15_09640"
     CDS             complement(6264..7580)
                     /locus_tag="VFC15_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10459.1"
                     /translation="MDALLLAIAVAIPCWYGYRRSVSHGLVEINHVTTLTFGFLYYWI
                     TPLAVRILAPQVDFPLDRVWLGLFREHLIAPYAVSGIALYVCFMVGDSLGARWWRRQR
                     TELARPIPTLALSLSTVFGCVLLVYSAYVTQADLFRPSVPGAIEVAMALGAVTAGVVL
                     LSCVALMFTLQQPETPWRKLLLSRYFLPPILGGSMMLLLGSRLYVASLVVMFAIYQTN
                     FRARFKLKRMIAVGLLFVVFFGLVGTWREGSSPTGVFSNVFVEPMLISLSLVHHLRYK
                     GIAWINQPTQLISDLENLIPTVLMPQKYKLLKQPDAYRPLGGLHSFVSFNLYFGLLGT
                     AAFWFLMPLGFRYLRSRLSDTLFATMYVMCSGWLTFSFFRNAFSLSLVKNIFEQSIVI
                     PFLIACFGWLLVAACSPASAGRIYAPPPQPEGARGETPLSPSASPP"
     gene            complement(7595..8884)
                     /locus_tag="VFC15_09645"
     CDS             complement(7595..8884)
                     /locus_tag="VFC15_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZM10460.1"
                     /translation="MSSSLRQLARNVVQLGSGEIPARLCGIAVLLLLARRSGVVMVGV
                     YALAQSMVQYSYPFIDFGLRHVGARLIAKHPEAGQEIVERVQRRRLLMASALLPFLLA
                     YALLANLPVKFRIFLFVFSAITCLYAVSLEWAAWGKEHLRLVGFARMIVPASILLFLL
                     LGGKSQRLLWWMIAGNAVGYGVQGVIFWEWWRRHRPAGERSISPAVVRESLAWARTSV
                     MGLAWFCNMAFNTIDMLMLGVMSNAHEVGLYSAAYRIMNQVLITYYLLTQTLYPKLAK
                     QQAEQRVRALRPGILVALGGVGLVLAFVLAVSSKAVLSVVFGQKFLPATLLLSLLAWA
                     IPLDFLTSYLSNAYIAWTMEKKVLTCTAISAASNVILNLIWIPTYGAEAAAVNTLVSY
                     VIFLASLAVAGWYSTELGRARTQTVGAGRTVINGMQE"
     gene            complement(9046..9339)
                     /locus_tag="VFC15_09650"
     CDS             complement(9046..9339)
                     /locus_tag="VFC15_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10461.1"
                     /translation="MPAAIAVVVVALCLNAADALRSAPDYLAYFNIFVKPENAWRLLT
                     DSNLDWGQGLIALRAYQQQHPQEPLYLAYFGPVAPRLYGIRATALPPTNGSKA"
     gene            complement(9463..10461)
                     /locus_tag="VFC15_09655"
     CDS             complement(9463..10461)
                     /locus_tag="VFC15_09655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM10462.1"
                     /translation="MQSFWFIGTQSLAYDEPGHIFAGLEAWQHGRLQMWIDHPPLGRY
                     WLTLPLARAHVQIGEESPYDRYPVTGMQPGPEWVAWRTRPMNTLLGVALGIALWFATR
                     RLFSEGAANVALALFAFTPSLIAHFSVATTDGIGALFIFLTACQLVRWRRNPGRKQTV
                     LMGLVLGGLLLAKLHTPPEMLLALALMLVLGCESILRRPRDWNWKPTLAALGIALLIL
                     WAGYLFHVSHVKVGNGQIVMTFPNRAARTLAIPSQTQLNLLVSAGEYAEGLRAVAISN
                     RSGRLAWFFGKIYPNGGPKLCYPAAIALKWPTVLLLLFFASCCSEFARRTALPAIC"
     gene            complement(10592..11470)
                     /locus_tag="VFC15_09660"
     CDS             complement(10592..11470)
                     /locus_tag="VFC15_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM10463.1"
                     /translation="MISLIVTTRNRVAELDRLLASLEAQTCQSFEVIVVDQNGDDRIL
                     EVLARHKGLRVRHLRSGMGASRGRNAGLRAFEGDLVAFPDDDCWYPSELLASVVQWFE
                     KNPDFDGLLTGIRNPDGKRMAPKFPPSEGPCSKHSILRCAIAWNIFLRARAAKSVGFF
                     REEMGPGASSPYQSGEDLDYPIRVLQRGFRLWYAPSLTVYHPELNSRDRRTTHKYAMS
                     VGRVWRLHDFSWGYCLSEIAFRSLGGAAFHLCRGDLRASSLYLERAAGQFRGYFREPE
                     HLTNSRESRAMGSRDA"
     gene            complement(11534..12808)
                     /locus_tag="VFC15_09665"
     CDS             complement(11534..12808)
                     /locus_tag="VFC15_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZM10464.1"
                     /translation="MESSRVSEAHTASGTGVYDRDVMSKLQPAVESPREVDAIAVLQV
                     LANNKMRILKVTLAAALLVLIVSLLLPKMYTATTTILPPQQNQSAATALVGQIGVLSG
                     LSGADLGLKNPSDLFVALLKCRSVQDGIVNRFDLRRVYWVKQFQDARKKLNSRSDIMA
                     GDEGLISISVSDRDPQRAAAMANAYVDQLRSLNQNLAVSEAAQRRLFYQEKLDAEREE
                     LAQSELALKEAQEKSGLIQPDAQSRAIIDAVAGIRAQVGIKEVQLQVMRTYATADNPD
                     LKRAEQELAGLRGQLAQLERSTGALGNGNLEIPTRRLPEVELDYIRRARELKYHEALY
                     EFLSKQLEAARIDEAKEAVVVQVVDKAVVPEKKSGPRRMLIVLVTTAAASFLSCLWVL
                     LAEALRRKQRDPQERERMALLRQSFKFSLKNS"
     gene            complement(12875..15193)
                     /locus_tag="VFC15_09670"
     CDS             complement(12875..15193)
                     /locus_tag="VFC15_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZM10465.1"
                     /translation="MKALPKENANLRNFWKLFAAVVVLSVSALTAAPGALAQNRPNAA
                     GMQTSPPTQMPVEEQTPAGVQTSPPSPTIVLDDTQTLPTANPPKAAPAAAMNSTKAVE
                     LTDFQRMVLSSLGKALPIYGLSLFENVPTTFAPVDRVPVTANYVIGPGDELLIRAWGQ
                     IDLDVHSRVDRNGNIFLPKVGNLNVAGLKYAQIEEFLKSHVGRIYQNFDLNVTMGELR
                     SIDVFVVGQARRPGRYTVSALSTLANAVFASGGPSPTGSMRHIQLKRGSTVVTDFDLY
                     DLLLNGDKTKDVLLLPEDVIYVAPIGPQVAMGGQVNVPAIYELKGGTDLAEALRLAGG
                     LSTTASGGKVFVEHIKDRQMRSVERVALDAAGLAHPIKDGDVVNVTLVSPRFANSVTL
                     RGNVAQPGRYPWHEGMRISDLIPNREFLITREYWKQQNAVALQAEESKGRVSSATNEV
                     RRNAPEINWDYAVVQRISSQDLSTQLLPFNLGKAILNNEDASNLVLQPSDIVTIFSQA
                     DLRVPEEKQTKLVRLDGEFEAAGIYRAKSGQRLRDLVMQAGGLTSAAYLYGAAFTRES
                     ARVEQQERLDMMIAQTEQQVARQGAQMAQNARTSDEVIAARSTLEAQRISLEKLRQLR
                     ADGRIVLNLHPNDQAVDTIPDITLEDGDQFYVPSRPIVVSVVGDVYNQGSFLQQPGKT
                     VTTYLRSAGGPTRDADKGRIFVVRANGAVVSKDAASHFWAGGFDSMVLMPGDSIVVPE
                     QTNKGSLLRGVKDWSQILANFGLAAAAINVLK"
     gene            complement(15307..15942)
                     /locus_tag="VFC15_09675"
     CDS             complement(15307..15942)
                     /locus_tag="VFC15_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM10466.1"
                     /translation="MKKTAGRSVTEVRIVEAAVQLFSRRGYKGTSTREIAHLAEVNEA
                     TLFRCFVRKADLFWAAAESRLNRLKLGRELQHGLATDLDPAIVVPMLATFVVENMSQH
                     PELMRLLYVAGFELPGADRMFREHLGPIFDAVNDYFGRCAARGVICDLEPTIATLGLA
                     GAVTAHQNLHHLFTGRDLPWDARDAAPAYAQFWLNALRQPVAASRVADSSD"
     gene            complement(17051..>19111)
                     /locus_tag="VFC15_09680"
     CDS             complement(17051..>19111)
                     /locus_tag="VFC15_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM10467.1"
                     /translation="INGPVQPLGTAPVTPGLFSTGSVPALPGTNNQYIFPGYVQNSTA
                     NAIPFGGPQNLYELYEDLSWTKGRHQFKFGGSYIQTRDNRVFGAYENAVQILGSNFTN
                     GLANLVAGQTYQFQGALYPQGKFPCSKNSNGVLQQTPDCTLNLPATEPAFNRNNRYND
                     GSMYVNDSWKVTPRLTLNLGLRWEYYGVQHNANPSLDSNFYLGSGSTFQEQIRNGSVQ
                     IADKSPVGGLWTQDKDNFAPRVGFAWDIFGDGSTSLRGGYGWGYERNFGNVTFNVIQN
                     PPNYAVISITSGIDVPTQPIYLNNIGAFGGTGTAPIPNVTLRTVRQDIPTAYTQFWSM
                     AVDRQLMRNSVLSVAYSGAKGSLLYDIGNINTQYFGSTYLGDTHLSNRLNYQYGNINY
                     RGNEGASLYNAVNVKFSTSNLFNKGLQLVANYTWAHSIDNQSSTFTDGYNGNYGLGYT
                     NYFNPNYDRGNSDFDVRQRFVVSAVWDLPWMKNASSAFARQALGGWSLSPIISVHSGY
                     PYNIYDGTNGLTINPRWIPGGPVPASGHNNGPVAPNQFNYLPMPVDPATGLAAGVGNA
                     LQVPTSGLVFCQNYAGASGSGAFSCNGAGITERNQTFGPGYWNADFLVTKQFKLTERF
                     NLQFRSEFYNIFNHSNLYINSNLLDLEYGATFIQAQKGGIYGNPGQPTDERRNIQFAL
                     KLLF"
BASE COUNT         4310 a         5553 c         5496 g         3752 t
ORIGIN      
        1 ggccaggaat agcgccattc gccaagccaa gggacagtgg ctggcctttc tcgatcaaga
       61 cgacgtctgg ctgaatggaa aattgcaaca gcagatggcc ttagcggacg accacgccgg
      121 catcatctac gggcgcgcca ttcagttcta tcccagcggt gaacagcggg actacgatta
      181 cgcgcatgaa tttcagccct tgcccgaagg cgccatcttc tcccaactct tcaccgacgc
      241 ttgcttcatc gccatgagtt cggcagtgct tcgccgctca gccgtggaag aggttggccc
      301 tgttcccgaa acgattcaaa gcgttccgga ttactatctc tatgtcgccg tggcgcgaag
      361 gtatccggcg cgggccgtgc aggaagcagt gtgtcgttat cgcatgcacg ccggcagcat
      421 gtcgcgaacg aaccggcaaa ggctgcacga ggaaccgtta ttgatcatcg atcaatgggc
      481 ggattgcctt gatccgggaa ttgttgcgca ccgtcgaatg acgtacagca ccgctttggc
      541 gctagaggaa atgcggagcc gcaaatctgc tcgcaaagga ctggtgcgaa tgctcacgga
      601 cggctctgta atttggctaa tgtcgcggcc gttggtgcgt gccttccgcg ctgtccggcg
      661 gagattgtgc cgcccctatt ggctgcgcac cgacttcgcg tctgatcggg actgagaaaa
      721 agaagcgcac gtggcctctt cgaggccctt accctttgct ccgcgtgcgc ccggttcgag
      781 caggagtgcg gcctgtgcgg ctatttcttt tttgggagcg ccgggtcctt tgataacccg
      841 cttatcggag ctcaagtaat atcccaatac tttggtgtag taacgggccc gaactccgaa
      901 gcgcaccagc gcttttgtaa ccctgctctt gccgaagtaa tcgtaggcct cactgaaatc
      961 gatcaatccc tgacgctgct cgcgaagtgc ccggagcaca tcctggctgg ctgtcttacc
     1021 gccgaagtgg accacctccg cacaaggcgt gtagaacacc ttgcatcctt tccggtgcaa
     1081 ggtgtagttg taggccagat caggcatggc ccagcgaaag cgttcatcca gcaaaccgta
     1141 ggtttcccaa gtggaccgac gaatgacata cgccgatgtg ccgatgctct ccactggctg
     1201 cgccttcgaa taatcgaagt cggtattgta gtaccggttc atcaacttgc ttccgggaaa
     1261 taacttgtgc caattgaggg tttgaagaaa gaagacagcc agactcgcga agctgcgaat
     1321 gtgatgctgg atgctgccat caggattaag cagcttggga ccgcagaccg ccgcctgggg
     1381 atgttcgtcc atgaagcgca ctaacggttc cagagcgttc tcaatcatta atgtgtcgct
     1441 gtccagcagg catacgtagc gcccgtgcga tctgcgaatg ccctgatttg cacttttcgc
     1501 atacgactcg ttgactgcat tcctgatcaa aatcacatca ggaaaaatct cggccaccat
     1561 atcggcgctg ccatccggag agttgccgtc aacacatatg acttcaaaag caattccgtc
     1621 ggtgtagcgg tagatcgaat cgagacagtt tcgcagcagc tctcgggtgt tgtagctggg
     1681 gatgatgacc gtaagatcga gaaatggctc tgtcattctg ggttccctgc cgatgatggt
     1741 gctgcccgcg ccggaacacc aaaaaccgtc tggttcggtt ctactgagtt gaggataacg
     1801 gaattcgcgc caacggtcac gttgtcgccg atttggatgc cccccaggac tttgcaaccg
     1861 gttcccagca tgacattgtt gccaatttgg ggccgcagcg cggggtcgaa cttcatatcg
     1921 gggcgcttgc tgcccagcgt gaccccttga aagatggtca cgttactgcc tacttgccag
     1981 gcgccgatca cgcttcccac cggatgggcg aagaagatgc ctgcaccaat ctcgcacttg
     2041 ggcgtaactt ccagcccgaa aagcacgagg ttcagatacg tcaggagctt cggcaagatc
     2101 ggaatgccgg cgagcattgc tgcccgactc gtccggcaca acacgttggg aaggaaacgg
     2161 aaatgcagca ggcgtccgag aaaacggacg aaatttggcc gaatttccga cctgccttcc
     2221 aattcgtatt gtcgagccag atcgctatac agcaattcct tgagacgagc catgttttcg
     2281 caaaatgccc agctttgaga gtaacagttt cgcttggccc cagacttgcc gcagccttat
     2341 tggcactcgc gatacaaggc cagcgtttcc cggccgcact tgtcccaact gtactttgcc
     2401 accacttcct tgcctcgctc caccgccagt tggcgtgcca tctcgtcgtt gaccgcctgc
     2461 agcagggcac ggttgaatga cccttgatcc atgggatcaa agtagaacgg tgcgtcctga
     2521 cagacctcgg gaatggatga cacctggctg gctaatacgg gacagccgac tgacattgct
     2581 tccaacggag gaaatccgaa gccttcattc aatgatggat acacgaacag atgggctccc
     2641 gcataagctt ccgccagcaa ctcgtcggaa acaatgggca gggcggtgag gcagtcgctc
     2701 atctcaagac tggcaatcag cgtcttttcc tgcactgtca gaggcccgcc gccgagcacc
     2761 agcaagtcga acgaatcatg cagccgggtt tcgtggaacg ccttcaacag tccagcgaaa
     2821 ttcttgaagg cgaccctcat cccaacatac agcacataat cgcgtcgcaa cttctgacgc
     2881 agactggcag cggcttccgc acttcgtgga agaggattga gaccatgatg aataacgcga
     2941 gtcttggccg gatcgactgc gtagaactgc aggagatcct gccgacagga ctcggacaca
     3001 cagatgatcg cgtccatttc ggggaaaagc ttctttcgcg cccagagcac tttcttgaca
     3061 tctggaaaga gatgcgggaa gcgctcgtga gtgcagtcat gatgggtggc gaccaggcgg
     3121 cgcgcgcgaa ccatcggcat gcgcatgaac aacgtgaggt gatagatgtc catggtgccg
     3181 cgaacgaaag ccgcgcaatt acccagaatt tcgttggcta cataacgcag cattccaggt
     3241 ggcagcgatt cgggaagctg cgtgactcgc gccttcgcgg gcggcaaact tcgaaacgga
     3301 tacaccgtgc cgctgattcc caacagcaat tccgtttgca catccggcac ggtgctcagg
     3361 aagttcatca gctcataaaa gtatcgggag gcgccacccg cgttctgtag cgagaaaact
     3421 tgatgatcgt agagaacgcg catgcctggg gccgctattg ccggtattcg cttcgccggg
     3481 gcgtggctcg atgagacgct cacagctcag gcgaccaatt gaccgaaggc gaagatggtg
     3541 agcccccaag cgcgtggcgc gaaccgctga tgcaggactc ccagcgcata tgccaatctg
     3601 gaaaacgtga gatagagcgg ttcggaggtg tgaccgtaca cggctgcccg aaaaccgtgc
     3661 gcgctcattt ggcggcggat cgaccggatc gtgttgcagc gtaaaaagtc gggaagacgt
     3721 cttccacgct ccgtgacttc tgcagccgcg acagcagcgc cgaatgcacg tggtgcgaat
     3781 gcaaagataa ccgccgtcga caagcactct gcgcgcctgc ttgaaaaaga actccggcgc
     3841 agcctgatgc tccagcaccg aatcgcagag gacaaggttg gcagatcatc acctccttcg
     3901 atccgagtgg ggcaagcctg cattctgcag agaaaagacc tgatgatcgt aaaggatcct
     3961 cattgcccgt cagcggaccc cgcagcgtgt ccggcgacat ccagtccgat gaaactgttt
     4021 gccgcggtgg caattgcgga cccgtgcctg cgttgtcacg cgccctcggc ctgaggagac
     4081 gtaaccaaaa tgacgccatt accactctcc gaacacgcgg ccgccaggag ccgcccgaag
     4141 cccacgatca cgattggaat gaggatggag tcttccagca tggttttgac cagcgagatg
     4201 gaagacggat cgcgaaactt atcttgagtc atgggtcgtg acgggcgcat cttcataccg
     4261 gaccaacgag agcaccttaa gaaccaaagg cgccggatgg ctctcaaccg tgacaattgc
     4321 cttgggacta ttgccagcaa tcacggtgta atgtttgtgt gccgctccat tcacctgatc
     4381 cggaaagcgg tttccggcat ttcccatgag atcaccggcc tcggtatcat cgagcaaccc
     4441 gcgcgcggtg atttgttgca ccatgttttg cgcaaccgca ccggttgcca cctgactcag
     4501 cgtgatgaac aggattagga ttacatagac caagattgtc gttccggtaa tatatttccc
     4561 gcttctgctc ttggtcatta caaagaagta ggccagcggc aaaacaaata acaggtatct
     4621 ttgcgcaggg cgcgtgaggg aaagtatggc tacaaatatg aaaatgccca gtaccatgca
     4681 taagatatag cgccgcgcgg aaggcgtgtt cgtattgagc aatatgtctt tcgacaggag
     4741 gataaaaatc gcggcaaaca ctaaaaaggt gccgctgtaa atccgcgggt tgatatatgc
     4801 gtccagtgga ccaaaccgca tttcttcccc aagccttacc ccatagactc ctgcgatgaa
     4861 aatagcaatg gttgcgacaa gggtgatcag cgctttccgg gtcgtattta cccgttccca
     4921 tacggcgctg agggaatacg ggaccagcag gagagacaga tatcctgcat acccaatcat
     4981 attcattacg atgctgatga gatttaggtt ggccccatga atcgcacgaa acaccggggg
     5041 caaaaaccag aatccgtaca attgctttgc gagaattata taggccgcag gaatcaccaa
     5101 tatcagggca acaataaaac tgtaacgcag aaatgctttt ctgatgttgg cgccgggtcg
     5161 ggttagggtc tccaaaccga cgagcggcaa caatagagct gcatgatatt taagcgtgat
     5221 ggcgatacca taggccgtaa tcgcgatggc gagccgcagg ggggggcttt ttgcttgcca
     5281 aaaaagtgtg atcgcaaaca gcgtcaacgc ggccggaaag agatcggcgg taccgcggcc
     5341 gccaaacgtc cagataagag gattgagaaa taccacagcc aatagaactg ccggattata
     5401 gtctcgctcg agaagagagc acagctttag cagtgagtac ccaaggagta gaaagcttga
     5461 cgtcgagaga acacgcagta ccgcattttg gtttagaaat ggcaagagac ggtgaaagac
     5521 agaggccagg aatggcatac ctaacgtatt tgcttgcagc aagaagtaat gcttgagaag
     5581 aactggatca tagcattcga agtatttcgc cccgtcggcg aatacccatt ccagattaat
     5641 tcccggcaac cagattaacg acaggtgcag cagtgttgcg atcagtatga ccaggaatgc
     5701 ggcacggctc tgagaggatg tctgggtcat gcgttattgt tgaaaccctg gatggaaatc
     5761 gcgtcctata atggttcttt ccgtcgttga aaagtcagct aggcctgatt tcaaagacga
     5821 cgcccgcgcc gcagaatgtc gtcccatata cattgctatt ggcgtcaagt acgacgctgc
     5881 ctgctagagt gtagaccgga atttccgttt tggctcccaa cagccccaca cgatacttga
     5941 gtttttgttg ttgaagctca cgggaggata tgtagttgtg aaaccgcacc accgcccgac
     6001 tcggcgtctc caggttggtc acgatgaatc cgactcgtgg cagcggaaga caaggaaaga
     6061 agccaaagct tacgattctc ggtgtgctcc gcgctggaga tccagtccgc tatcgcgctc
     6121 caagcgtcgt ggcaaattac tggcagtcgc gatacacggc cagcgtcttg cgcccgcact
     6181 tttccctgat gatcgtagag aatcctcatg ggtctgcgga gaaagccgca acgcctgcag
     6241 cagcatccct gtgtcgagag tgcctagggt ggtgatgcgg agggcgaaag cggcgtttcg
     6301 cctcgggcac cttctggctg gggcggcgga gcgtaaatgc gacccgcact agctggggag
     6361 caagctgcaa ccaggagcca gccaaaacac gcgatcagaa acgggatgac aatggactgt
     6421 tcaaagatgt ttttcaccag tgagagggag aatgcattgc ggaagaagct aaacgtcagc
     6481 cagcccgagc acatcacata catggtcgcg aataacgtat cggacaggcg gctcctcaga
     6541 taccggaaac ctagcggcat aagaaaccaa aaggcagctg ttccgagtag cccgaagtat
     6601 agattaaaag aaacgaacga gtgaagcccg cccaggggac ggtaggcatc gggctgttta
     6661 agcaatttat atttttgcgg catcagaact gtcggaatca ggttttccaa atcgctgatc
     6721 aactgagtag gttgattgat ccaggcaata cccttgtatc ggaggtgatg gacaagcgaa
     6781 aggcttatca gcataggttc cacgaatacg ttggaaaaca cgcccgtcgg gctgctgcct
     6841 tcgcgccatg tacccaccaa tccaaagaaa acaacaaaaa gaaggcccac agctatcatc
     6901 ctcttgagct tgaatctggc gcgaaaattg gtctggtaga tggcgaacat caccaccagg
     6961 gacgcgacat aaagcctgct gcccaggaga agcatcatgc tgccccccag gatcggcggc
     7021 aggaagtagc ggctgagcag gagcttgcgc catggcgtct cgggctgctg cagggtgaac
     7081 atcagggcca cgcagctcaa cagcacaaca ccggcggtaa ccgccccgag tgccatagcg
     7141 acctcaatag cgccgggcac cgagggtcgg aagaggtcgg cttgtgttac atacgccgaa
     7201 tagaccagga gcacgcatcc gaaaaccgta gaaagtgaaa gcgcgagcgt gggaatcggc
     7261 ctggccaatt cggtccgctg gcgacgccac caccgggcgc cgagcgagtc tcctaccatg
     7321 aagcagacat aaagggcgat gcccgacacc gcataaggag cgatcagatg ctcacgaaat
     7381 aatcccaacc acacccggtc caaggggaaa tctacttgcg gcgccagaat tctgaccgcc
     7441 aacggcgtga tccagtaata caaaaaaccg aaggtgagcg tggtgacgtg attgatctcg
     7501 accaacccgt gcgataccga tcgccggtag ccgtaccaac agggaatcgc caccgctatc
     7561 gcaagcaaca aagcgtccat cggccggctc cgaattactc ctgcataccg ttgataacgg
     7621 tgcgacccgc gcccaccgtc tgcgtccggg cacggcccaa ctcggttgag taccagcccg
     7681 caaccgccag gctagccagg aagatcacgt aggagacaag ggtgtttacc gccgccgcct
     7741 ccgccccata ggtcggtatc cagatcaaat tcaggatgac gttgctggcg gcggaaatgg
     7801 cggtgcaggt taacaccttc ttctccatcg tccaggcgat ataggcatta cttaggtacg
     7861 aggtcaggaa gtccagcggt atcgcccagg ccagcagcga aagcagcaag gtcgcaggca
     7921 agaacttctg tccgaagacg acggaaagca cggccttgct ggagactgca aggacgaagg
     7981 caagaaccag gccgacaccg ccgagcgcga ccagaatgcc gggacggaga gctcggacgc
     8041 gctgttcggc ctgctgcttg gcaagtttgg gatagagagt ctgggtcagc agatagtagg
     8101 taatcaacac ctggttcatg atgcgatagg cggcgctgta gagcccaacc tcgtgcgcat
     8161 tggacatgac gcccagcatc agcatgtcga tggtgttgaa ggccatgttg cagaaccacg
     8221 ccagccccat gacgctggta cgcgcccagg ccaacgattc ccgaactacc gcgggagaga
     8281 tgcttcgttc gcccgcgggc cggtgcctcc gccaccattc ccagaagatc acgccctgaa
     8341 cgccgtaacc gacggcattg cctgcgatca tccaccacaa gaggcgttgg ctcttgcctc
     8401 ccagcagcag aaacagaaga atgcttgcgg gaacgatcat ccgggcgaag ccgaccaggc
     8461 gaaggtgctc tttgccccag gctgcccact ccagcgacac ggcgtacaga caagtgatcg
     8521 cagagaagac gaagagaaaa atcctgaact ttaccggcag attggccagg agcgcatagg
     8581 ccagcagaaa cggaagaagg gcagaggcca tcagcagcct tcgcctctgc accctctcga
     8641 ctatctcctg gcctgcttcc ggatgcttcg cgatcagccg tgcgcctaca tgtcgcaaac
     8701 cgaaatcgat gaacgggtag gaatactgca ccatgctctg ggccagtgca tagacaccca
     8761 ccatcaccac cccggagcgg cgagccagca gcagcaggac ggcgatgccg cacaggcgcg
     8821 ccggtatctc gcccgatccg agttggacta cattgcgagc gagctgccgc agagaggaac
     8881 tcatcgacta ccccttctga ctccgcccat ccttctcatt tcgtcgccgt gtcgaacacc
     8941 cacagagagc gatccagcac gcgctgcggt cgataaaact gcaaccagga tagctgccga
     9001 cctggtctaa tacctgaccg gtaaggcaac tcgcaccggc gacaattatg cctttgaccc
     9061 gttcgttggg gggagagcag ttgctcgaat gccgtacaac cggggagcga ccgggccgaa
     9121 gtaggcaaga tacagaggtt cttgcggatg ttgctgctgg taggcgcgca gcgcgatcaa
     9181 tccctgtccc caatccaggt tgctgtcggt gagcagccgc caggcgtttt cgggcttcac
     9241 gaagatattg aagtacgcca gataatcggg cgcggagcga agagcgtccg cagcattcag
     9301 gcaaagcgca actaccacga ccgcgatggc ggcgggcatg ctcccagatg ccgcccgcaa
     9361 tgagcaacgc aaacggatac agcggcaaaa gatgccgctc gccgatcgtg atgcgcgact
     9421 gcaacgcgaa ggcgaggaac accagcccga acagcgacat aatcagcaga tcgctgggag
     9481 cgcggtacgt cttgcgaact ccgaacagca actagcgaag aacagcagca gcaaaaccgt
     9541 cggccacttc agcgcaatcg cggcgggata acaaagtttg gggccgccat tggggtatat
     9601 cttcccaaag aaccatgcga gacgcccgct acggttgctg atcgcaaccg cccgcaggcc
     9661 ctcggcatac tcgccggccg agaccagcaa attcagttgc gtctgcgatg ggatggccag
     9721 cgtcctcgcc gcacgattgg ggaatgtcat cactatctgc ccgtttccaa ccttcacatg
     9781 cgagacgtga aacagatacc ctgcccacag gatgagcaag gcgatgccga gcgcagcgag
     9841 ggttggcttc caattccaat cccgaggccg ccgcaaaata ctttcgcacc ccagtaccag
     9901 catcagcgcc agggccagca gcatttccgg aggcgtgtgc agcttggcca gcagcaaccc
     9961 accaagcacc agccccatca gcacggtttg cttgcgcccc ggatttcttc tccagcgcac
    10021 gagctggcag gcggtgagaa agatgaacag tgcgccgatg ccatcggtag tcgcgacgga
    10081 gaaatgcgcg atgagcgatg gtgtaaaagc aaacagcgcg agcgcgacgt tggccgcgcc
    10141 ctcggaaaac aagcggcgcg tggcgaacca cagcgcgatg cccagcgcca cgcccagcag
    10201 cgtgttcatc gggcgggtgc gccaggccac ccattccggg ccgggctgca tgccggtcac
    10261 gggatagcga tcgtatggcg actcctcccc gatctggacg tgcgctcgcg ccagcggcaa
    10321 cgtcagccaa tagcgcccca ggggcgggtg gtcgatccac atctgcaagc ggccgtgctg
    10381 ccatgcttcg agtccggcga agatgtgacc gggttcgtcg taggcgagcg actgcgtgcc
    10441 gataaaccag aacgattgca ccgcaaaaac ctcagcagca gtgccggaat ccagcgcgca
    10501 gggcgccttg cagcagggac ggtgctgcca accggctcta gttcgctgaa accgtcactg
    10561 gagttggtct ccagcggctg ggaacgagag cttaggcatc acggcttccc atggcccgcg
    10621 actcccgcga atttgtcagg tgctcgggct cgcgaaaata tccgcgaaac tggcccgcgg
    10681 cccgttccag atagagagaa cttgcacgca agtcgccgcg gcatagatgg aacgccgctc
    10741 cgccgagaga acggaaggcg atctcgctca gacaataccc ccaggaaaaa tcgtggagac
    10801 gccacacccg cccaacgctc attgcgtatt tgtgggtggt tctgcggtcc ctcgagttca
    10861 gttcgggatg atagaccgta aggcttgggg cataccacag ccggaagccg cgctgcaaaa
    10921 cgcgaatggg ataatcgaga tcttctcccg attggtaggg ggaggacgcg cccggaccca
    10981 tttcctctcg aaaaaagcca acgcttttgg ctgcccgggc ccggagaaag atattccaag
    11041 ctatggcaca gcgtaagatg ctgtgtttgc tgcagggacc ctcagagggc ggaaatttag
    11101 gcgccatccg cttcccgtct ggattacgga tcccggtcag caggccgtca aagtcgggat
    11161 tcttctcgaa ccattgaacg acgctggcca ggagttcgct gggataccag cagtcgtcgt
    11221 cgggaaaggc gacgaggtcg ccctcgaaag cgcgaagccc ggcgttacgc cccctcgaag
    11281 cccccattcc gctgcgcaga tgtcgcaccc tcaggccctt atgtctcgcc agcacctcga
    11341 ggatgcgatc gtcgccattc tggtcgacaa caatcacctc aaacgattgg caggtctgcg
    11401 cctccaggct ggcgagcaac cgatccagct ctgccacgcg gttgcgggta gtgacgatca
    11461 acgaaatcat gatgaatccc gaggcgaccg gagaagaaac cgggcctcga cggggccctc
    11521 actgccggga gagttaggaa ttcttcaacg agaacttaaa cgactgacgc agcaaggcca
    11581 ttcgctcccg ctcctgtgga tcgcgctgct tgcgccgcag cgcttccgca agcaataccc
    11641 ataggcagga caggaaggaa gccgcggccg tcgtcaccag tacgatcagc atccggcgcg
    11701 gccccgactt cttttccgga accaccgcct tgtccaccac ctgcaccacc acggcttcct
    11761 tggcctcatc gatgcgggcg gcctccagtt gcttgctgag gaactcgtac agcgcctcgt
    11821 gatatttcag ctcgcgtgcc cgccggatgt agtcgagctc aacctcgggc agacgccggg
    11881 tgggaatctc cagattgcca ttacccagcg cgcccgtgct gcgctccagt tgggccaatt
    11941 gtccgcgcaa gcccgccaac tcctgctcgg cgcgcttaag atcggggttg tcggcggtgg
    12001 cgtaggtgcg catgacctgc aactgcacct ctttgatccc cacctgcgcg cggatgccgg
    12061 caacagcatc gatgatggcg cggctttgcg catccggctg gatcaatccg ctcttctcct
    12121 gcgcctcttt caaagcgagc tctgactgcg ccagctcttc gcgctcggca tccagctttt
    12181 cctgatagaa cagccgccgc tgcgccgctt ccgataccgc caggttctga ttgagcgagc
    12241 gcaattggtc cacgtaggca ttcgccatcg ccgccgcccg ctgggggtcg cgatcgctca
    12301 cgctgatcga aatcagtccc tcgtcgccgg ccataatatc gctgcggctg ttcagcttct
    12361 tgcgcgcatc ctgaaactgc ttcacccaat acacccgccg cagatcgaag cggttgacga
    12421 tgccgtcttg caccgaccgg cacttcagca aggccacgaa caggtccgat gggttcttca
    12481 agcccaggtc cgccccggac aacccgctca aaacgccaat ttgtccgacc agcgctgtgg
    12541 ccgccgactg gttctgctgc ggcggcagaa ttgtggtggt cgcggtgtac atcttgggaa
    12601 gcagcaggga aacgatcaag accagcaatg cagcagccag cgtgaccttc aagatccgca
    12661 ttttgttgtt tgcgagcacc tgcaacactg cgatcgcatc gacttctctc gggctctcga
    12721 ctgcaggctg cagcttcgac atgacgtcgc ggtcgtaaac tccggtgccg ctagcggtat
    12781 gcgcttccga tactctcgag ctttccataa tagggaccta cttccctggt cgtgattcgc
    12841 gccaaatttt tctgcgacgc tagtgtttcg gcaatcactt caagacgttg atggcagccg
    12901 ctgccaatcc aaagttggcc aggatctgcg accagtcctt gaccccgcgc agcagactgc
    12961 ctttgttggt ctgctccgga accacgatgc tgtcgcctgg catcagtacc atgctgtcga
    13021 agcctcccgc ccagaagtgc gaagcggcat ctttgctgac gaccgcgccg ttggcgcgaa
    13081 ccacgaagat cctgcccttg tccgcatccc gcgtggggcc gccggcgctc cgcaaatacg
    13141 tcgtaacggt cttgccgggt tgttggagga aggacccctg gttgtagacg tcgccgacga
    13201 cactcaccac gattggccgg ctgggtacat aaaactgatc gccatcctca agcgtgatgt
    13261 ccgggatggt atccacagcc tggtcattag ggtgaaggtt caaaacaatg cggccgtcgg
    13321 cccgcagttg gcgaagcttt tcgagactga tgcgttgcgc ctccagggtg gacctggcgg
    13381 caatcacctc atcggaagtc ctcgcattct gcgccatctg cgccccctga cgggcgacct
    13441 gttgctccgt ctgggcaatc atcatgtcca gccgttcttg ttgctccacg cgcgccgatt
    13501 cacgggtaaa cgcggcgcca taaagataag ctgccgaggt tagccctccc gcctgcatca
    13561 ccaaatcgcg aagcctctgg cccgatttcg cccggtagat tccggcggct tcaaactccc
    13621 catccagcct gaccagcttg gtttgttttt cttcgggaac gcgcaaatcg gcctgcgaga
    13681 aaatggtgac gatgtcgctt ggctggagta cgagattgct ggcatcttca ttgttcagaa
    13741 tggccttgcc aaggttgaag ggcaaaagct gtgtcgaaag gtcctgcgaa ctgatgcgct
    13801 gcacaaccgc ataatcccag ttgatctccg gggcattgcg cctcacctcg ttggtggcgc
    13861 tgctcacccg gcctttactt tcctccgcct gcagagccac agcattctgc tgcttccagt
    13921 attcccgggt aatgagaaac tcgcggttgg ggatcaaatc gctgattcgc atgccttcgt
    13981 gccacggata gcggccgggt tgggcgacgt tgccgcgcaa ggtcacggaa tttgcaaagc
    14041 gaggagacac cagggtgaca ttcaccacat cgccatcttt gatgggatgg gccaagccag
    14101 cggcatccaa cgccactcgc tccacgctgc gcatctgccg gtccttaatg tgttccacaa
    14161 agactttgcc gccagacgcc gttgtggaca gcccgcctgc caggcgaaga gcttccgcta
    14221 agtcggtccc gcctttgagc tcataaatcg cgggcacatt cacctgtcca cccatggcaa
    14281 cctgaggccc gataggggcc acgtagatca catcttcggg caacagaagg acgtctttgg
    14341 tcttgtcgcc gttgagcagc aaatcgtaga gatcaaaatc ggtaaccacc gtggaaccgc
    14401 gcttcagctg gatatgtcgc atggaaccgg tcggtgacgg tccgcctgag gcaaagactg
    14461 cattcgccag cgtgctcaaa gcgctgaccg tataacggcc cgggcgccgt gcttgaccta
    14521 ccacgaagac gtcaatcgag cgcagctcgc ccatagtcac gttcagatcg aaattctggt
    14581 agatgcgccc cacgtgtgac ttaagaaact cctcgatctg ggcgtacttc agcccggcca
    14641 cgtttaggtt cccaactttg gggagaaaga tgttgccgtt gcggtcaact cggctgtgaa
    14701 cgtccaagtc aatctgtccc caggcccgaa tcagcaactc gtcgccggga ccgatgacgt
    14761 aattcgccgt gacgggcacg cgatccacgg gagcaaacgt tgttggcaca ttctcaaaga
    14821 ggctcaggcc gtagatgggg agcgccttgc ccagagaaga aaggaccatc cgttgaaagt
    14881 cggtgagctc gacagctttg gtagagttca tcgccgcggc cggcgcagcc ttggggggat
    14941 tcgcggttgg cagggtctgg gtgtcgtcca gcacgatagt gggcgaaggc ggagaggtct
    15001 gcacgccggc tggggtttgt tcttccactg gcatctgggt tggcggcgaa gtctgcatcc
    15061 cggctgcatt cggccggttc tgcgcgagag cgcccggtgc agccgtcaga gcacttacgg
    15121 acagcaccac cactgcggca aaaagcttcc agaagtttcg tagatttgca ttctctttcg
    15181 gaagagcttt caatctcggt ctccccaaaa cccaagttgg gtcctgtacg aagtcgttac
    15241 ttgacgaggc aaaccagcag ccccgaggaa tctcttgctt ggagcaggta gaaattctgc
    15301 ggcgatctag tcgctactat cggcaacccg gctcgccgca accggctggc ggagagcgtt
    15361 cagccagaac tgagcgtagg ctggggccgc atcgcgagcg tcccacggta ggtcgcgccc
    15421 ggtaaagagg tggtgcaggt tttggtgagc ggtcaccgcg cctgccagtc cgagcgtggc
    15481 gatcgtgggt tcgagatcgc aaattacgcc gcgcgccgcg cacctgccga agtagtcatt
    15541 caccgcgtcg aagatcggtc ccagatgttc gcgaaacatc cggtccgcgc ccggcagttc
    15601 aaacccggca acatacaaaa gccgcatcag ttcaggatgc tgcgacatat tctccaccac
    15661 gaatgtcgcc agcatgggga caacgatggc tggatccaaa tccgtcgcca ggccatgttg
    15721 cagctctcgc cccagcttca accggttcag ccgcgattcg gccgccgccc agaagaggtc
    15781 agctttccgc acgaagcagc ggaagagcgt ggcttcattc acctcggcta gatgggcgat
    15841 ctcccgtgtg ctggtaccct tgtatccccg acgggagaag agctgcacag cggcctcgac
    15901 gatacgcact tccgtgacac ttcttcccgc tgtttttttc acgatcttca atccttaagc
    15961 tgtgtatcga aaggtacgat gggtggctgt cgtttgacgg gcccgatctc gccgctgcca
    16021 acttcgacag cgacgctgcg catgatcacg tccaaggtga gcacaactcg gagctgttct
    16081 tcttgctgac cagcacaccg accatgccca ctagcgagcc agaattgatg cgaaccttgt
    16141 tcccggcaac caggtatggg tggggctcga atttacgcag agccagggct tcacggagcg
    16201 attcaatctc agaagtgggc aaggccgacg ggtcgcctct tgaaccacaa tcgactgcac
    16261 gccggaaggc tctccccata atgggcaagg ctctgccatc ctcagttaga tgaggtgttt
    16321 tccaaacgtc ccgcgaggta ctgccaggaa ccacttcgcg aacatttcca accaactcga
    16381 gccccggtga actgtgccgc tccccccgcg agtaaaagcg gagagcaagc acatacttac
    16441 tacgaccggg cgcacttgct gccaagttgt gactctactc gacgctcgca agcataggga
    16501 aagctgctca atcggtcaag taaaaacggc tcaaatcaat caatcggcgc cggccaaaga
    16561 tctttgagtg gcgagaccat gaataagacg atggcgtgga accgggaaac tacggcgagc
    16621 gcccacttcg accgtcaacg gagcgagcgg tccccggccc tccgtggggg aacggcccaa
    16681 tcgccagacc caagggctcc gcgcatgacc tcacccgaac acacgaccac gcgtcgcgcc
    16741 gctttggcgg cagacgggtt gacggaatga caacaagagg gacaacaccg cggcgaatgt
    16801 ctctttggcg tagaaaccga actgctcccc tcatgacgta gttggatgca agttgcaatg
    16861 gcgggtttgc gcctggttcc gagttgctcc gcgggagacc gcctgtcgcg cagcctgacc
    16921 gaatccgggg aagggtaagt cgttccgcgc tcaagcatca tccaaaagta ttgcgacggc
    16981 cagtaccgta catagcgaca aaaatgggga gcccgaaagc tccccgagta ctgcaagttc
    17041 gatcagccgg ctagaacaac agcttcagag cgaactgaat gttgcgccgc tcgtcggtcg
    17101 gctggccagg gttgccgtag ataccgccct tctgggcctg aatgaatgtt gcgccatatt
    17161 cgaggtctag aaggttggaa ttgatgtaaa ggttactgtg gttgaagata ttgtagaact
    17221 cgctgcggaa ttggaggttg aaccgctccg tcaacttgaa ctgcttggtg acgaggaagt
    17281 ccgcgttcca gtatcccggt ccgaaggtct ggttgcgttc cgttatgcca gcgccattgc
    17341 agctaaaagc tccgctcccc gaggccccgg cgtaattctg acagaaaacg agccctgatg
    17401 taggcacctg cagcgcgttg cccacacccg cagcgaggcc tgtagcagga tccaccggca
    17461 tcgggagata gttaaactgg ttcggggcta ccggaccatt gttgtggcct gaggctggaa
    17521 ccggcccgcc ggggatccaa cgcgggttga tggtaagacc gttggtgccg tcgtaaatgt
    17581 tgtagggata ccctgaatgc accgagatga tgggcgacag gctccaaccg cccagcgcct
    17641 gacgcgcgaa agcgctactg gcattcttca tccatggcaa atcccacacg gcactcacaa
    17701 cgaagcgctg acggacgtcg aaatcggaat tccccctgtc ataattcgga ttaaagtagt
    17761 tggtgtagcc caacccgtag ttgccgttgt agccgtcggt aaacgtggac gactggttgt
    17821 caatggaatg cgcccaagtg tagttagcga ccaattgcag tcccttgttg aagagattgc
    17881 tggtagagaa cttcacattc acagcattgt agaggctggc cccctcattg ccacggtagt
    17941 tgatgttgcc gtactggtaa ttcaaccgat tgctgaggtg tgtatctccc aggtaggtcg
    18001 agccgaaata ctgtgtgttg atgttgccaa tgtcgtacag aaggctcccc ttggcaccgg
    18061 agtaagccac ggacagtacc gagttcctca tcagctggcg atcaactgcc attgaccaga
    18121 actgcgtgta tgcggtcggg atgtcttgcc ggacggtacg cagggtgacg ttgggaatag
    18181 gcgccgtacc ggtacccccg aaggcgccga tattgttgag gtagatgggc tgggtgggaa
    18241 catcgatacc cgacgtgatg ctgatcaccg catagttcgg cgggttctga atgacgttga
    18301 aggtcacgtt gccaaagtta cgttcgtagc cccagccgta gccgccgcga agactggtgc
    18361 ttccgtcgcc gaagatgtcc caggcgaagc ccacgcgcgg cgcgaagttg tccttatcct
    18421 gcgtccacaa cccgccaact ggactcttgt cggcaatctg aacgctgccg ttgcgtatct
    18481 gttcctggaa ggtgctgccc gaacccagat agaagttgga atcgagactc ggattggcgt
    18541 tgtgctgcac gccgtaatac tcccagcgca ggcccaggtt caaggtcagg cgcggggtga
    18601 ctttccagga gtcgttaacg tacatcgatc cgtcgttgta tcggttgttg cggttgaacg
    18661 ccggctcggt cgcaggcagg tttagcgtgc aatcgggagt ttgctgtaaa acgccgtttg
    18721 agttcttaga gcacgggaat ttgccctgag ggtagagagc gccctggaac tggtaggtct
    18781 ggccggcgac caggttagca agtccattcg tgaagttgct tccgagaatc tgcacggcat
    18841 tctcgtaagc gccaaacacc cggttgtcgc gcgtctggat gtaactgcca ccaaacttga
    18901 actggtgcct gcctttggtc caagagaggt cctcgtacag ttcatacagg ttctggggac
    18961 cgccaaacgg aatggcgtta gctgtcgaat tctgtacgta gcccgggaaa atatattggt
    19021 tgttcgtccc tggcagcgcg ggaactgagc cggtgctgaa caggccaggc gttacaggag
    19081 cagtgcccaa cggctgcacc ggaccgttga t
//
LOCUS       DASRKK010000417         7856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1010031, whole genome shotgun sequence.
ACCESSION   DASRKK010000417 DASRKK010000000
VERSION     DASRKK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010031"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            890..1153
                     /locus_tag="VFC15_09685"
     CDS             890..1153
                     /locus_tag="VFC15_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10468.1"
                     /translation="MASIEALPYWSIVVEMCACRMSFFRWDRPERKEIVVALDRFELR
                     EQCQIRAISGQGFNETGDFLAGGVENTVVAFRVIKAISECPIG"
     gene            complement(1250..1639)
                     /locus_tag="VFC15_09690"
     CDS             complement(1250..1639)
                     /locus_tag="VFC15_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10469.1"
                     /translation="MAATTQPRPGLQAYRESLSLAFSDLVASLANSIGRKLTAHVAGV
                     KDVRALDRWMEGKEPYGNAEPRLRLTFQVVRTLLDHDPPNVVQAWLTGVNPELGDRVP
                     LRLLREGELDVIAPEVLAAARAFISGG"
     gene            complement(2007..2795)
                     /locus_tag="VFC15_09695"
     CDS             complement(2007..2795)
                     /locus_tag="VFC15_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10470.1"
                     /translation="MSFGSSALTELAQTLVEIDQANESDWIAAGKTPSTLVEHVLRRY
                     LADRGQSTIAEHFELTLTLGEAIVDSIYGEAEPTTSGQLFFVLNTESSFALGVGEAIT
                     ELEGAQAGMGRAFYDGLRQALYRWIRVYDDLDARERIEQMTEWAEGEDDPESYEIPKL
                     EQDLPPSLKDERRSNTARSLTSFPVPDVPGLRELVEATIELHRVSHSMERPRLDDEWL
                     ERERHYHSLYRCPLSCFTSGRATPSWRASTTNANTGARRRRNPT"
     gene            complement(2967..4031)
                     /locus_tag="VFC15_09700"
     CDS             complement(2967..4031)
                     /locus_tag="VFC15_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10471.1"
                     /translation="MILIAPHIAAFFQQRLPIERRASANTCDSYAHAFRLFLEYASGC
                     LNVSPSQLQLEHLDAALVLNFLNHLETTRMNSAGSRNTRLAAIKSFMRFMEYRMPAAL
                     DQIRRILAIPVKKTDARLVRHLTAQELRAILDVPNPTVRDGIRDRAMLHLCFAGGLRV
                     SELIGLHVNDVTLQPEANVLVHGKGRKERCLPLWKETVSALRAWLAVRGGALVPELFL
                     NARNQCMTRSGFEYILRKHVRTAQESCPSLSSKRVSPHVLRHTCAMTILQATKDLRKV
                     SLWLGHANMQTTEIYTRADPTVKLETLESIIAPKLRTGRFKATDQLIAQLKNATIMQS
                     KRVSQSAGAGGGRKPPPHKN"
     gene            complement(4028..4936)
                     /locus_tag="VFC15_09705"
     CDS             complement(4028..4936)
                     /locus_tag="VFC15_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10472.1"
                     /translation="MLVDAVESYLAVRRATGFALKCTGSLLHSFAVFSEALGKDYVCA
                     QTAIQWAGLAQSLPTRAHRLGQVIRFARYIRAEDQRNELPPAVFGSDTRPRPIPYIFS
                     TENIRRLVQAASRLGSYSFRRGTYSTLFALLACTGLRVSEAIGLRYDDITADGLVIRR
                     SKFRKSRIVPLHHTARMALQEYLQRRRPYAPFDDHVFVSLRRRLLLRTDVETAFQSAV
                     KQLGLPSGPRLPRPTVHSLRHTFAVRALETCPDGRDRITKHMLALSTYLGHGKVANTY
                     WYLQTTPQLMKTIADSTQEFFSGGRP"
     gene            complement(4930..5916)
                     /locus_tag="VFC15_09710"
     CDS             complement(4930..5916)
                     /locus_tag="VFC15_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10473.1"
                     /translation="MRRRSLPHAARLFLDYLRRSGVLTAAHIEPVTHDPILVVSFCEW
                     MRQQRGTCDDTVKNYCLDLRALLKKLGEDPAKFDAQNLRKFVLERSQRHGWSPAKRCT
                     TAVRMFVRFLIAEGKCSAGLDAAIPTWAHWRCSSLPRYLQSDEVERVIASCAPNTHVG
                     VRDRAILLLLARLALRAGDIIQLRLGDIDWKEAWICVCGKGRRQTRLPMTQEVGDALA
                     GYISQARHPADTDALFIRSRAPMRPFQNQNAVSAIVGRAMRRAGVSSPSRGAAHVLRH
                     SAATSMLRHGASLQEIGVILRHRSIAATEIYAKVDFAVLQQIAQPWPEVTTC"
     gene            6496..7377
                     /locus_tag="VFC15_09715"
     CDS             6496..7377
                     /locus_tag="VFC15_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10474.1"
                     /translation="MTSLRLRMLEDMQIRNLAVNTQESYVQQVSLFARYFHQSPERLG
                     PEQIRAYQLYLTKEKKLATGSILIAISALRFLYKVTLKKGWTFEDIIPAPKKPQTLPM
                     VLSPEEVMQFLTCVASRKHRVILTTCYAAGLRVSESIALTPPAIDSKRMVLRVEQGKG
                     MKDRYVMLSPKLLQILREWWRVERPEHWLFSGDMPGQHISRGAVELECQKARRLCKIS
                     KPITPHSMRHAFAVHLLEQGTDVRTIQLLLGHRSLATTAKYLRIATSKVCSTSSPLDL
                     LPHPIPQEVKPTPPPLF"
     gene            7390..7856
                     /locus_tag="VFC15_09720"
                     /pseudo
     CDS             7390..7856
                     /locus_tag="VFC15_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612315.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT         1742 a         2253 c         2194 g         1667 t
ORIGIN      
        1 gcacgagcaa ggggcggatc agcactgcga agttgccgct cctggttatc gggattcttg
       61 tatctacaac tggcttgacg aaatactggc gtgagagccg gatggaaact gggttctcgt
      121 tcgttcctct gggcagtcga atgcccgata agtcgcgttt acatctaggc ccgactcaac
      181 ttggagtgaa ataagtcccc gtaaacgccc cagggtctac gaatgctatt gggcgcattt
      241 cgcggcggaa atccacttga cgcatagtcg cctatgcgcg gaagtgccag ctggtgtcaa
      301 ctcccgagat accggcatgt gggaagtgtc ggatcaggct tccgtacgga tggttagcaa
      361 tctgaagtga tgccaatctc gaaccgtcgc ttgatgaaaa atcgccgagt cgtttattgc
      421 ccggtccagt tcaattaaac ccctgtcgca gtcaaccgct catttcgcca gcccacttcg
      481 ccactttgcg caaggcatct tatcggcgtc ggcaatccac ctgaatggcg gcatagaaca
      541 gtgccaagta accgtccctc gaagtcggcg cgcccaaaag ggcaatacga gggcaattgc
      601 aattccgaag gctccccaat gaggacgtta cgttactctt tgcgccgcgt aagtccatta
      661 gatttcgtga acagtagcac tcataaccca aaggtcggtg gttcaaatcc accccccgca
      721 accaacggaa tcatagggtt acgggcaatt gggcacttgt agcgactgtc cataactgtc
      781 caataaggga aactggctca cagtttccct tatttattga acgaaaactg tctcgttggc
      841 agccgttctt ttcaagagtc gttgcacgac aggccgattg gctttgcgct tggcttccat
      901 cgaagcattg ccatactggt ccatcgtggt ggaaatgtgt gcgtgtcgca tgagcttctt
      961 ccgctgggac cgtcccgaga ggaaagaaat cgttgtcgcc ctcgatcgct tcgagctccg
     1021 cgagcagtgt cagatccggg cgatatctgg ccagggtttc aatgaaacag gagacttcct
     1081 ggctggaggc gtagagaaca cggtagtagc tttccgggtc atcaaagcga tttccgaatg
     1141 tcccatcggc tgacgccctc gaccaatccg gcgggcgcca ggcatcgggc tgccgtccaa
     1201 tgcggtagat cggttgcgga ggggttttcg tttccagcgc catggctcgc taacccccgg
     1261 agatgaatgc cctcgcagcg gcaaggacct ccggagcgat tacgtcgagt tctccctcac
     1321 gtaagagtct taacggcacc cggtctccca actccgggtt gacgccggtc agccaagcct
     1381 gcaccacgtt cggtgggtca tggtcgagca atgtgcgaac gacctgaaac gtcagccgca
     1441 gccgcggttc cgcattccca tagggttctt tcccctccat ccacctgtcc aaagcacgta
     1501 cgtctttcac gcccgccaca tgcgcggtca gcttgcggcc gatcgaattc gccaaggacg
     1561 caaccaggtc cgaaaaagcc agactcaacg attcacggta tgcctgtaga ccgggacgag
     1621 gctgtgtagt ggcggccatt gcctacctcc aacaaaatca tggcataatc actcaagata
     1681 gtcaatataa taacgtaaaa atagcacatg aaaaccaaat ccaactaatt gactgtgagt
     1741 ggatgcccag ggcggggtct cttcctctcc gggcgtggcg cttcgaccgc aaagcacgtc
     1801 gaggtcacac accgcaccgc tgccctttct ttagtaggac agcagtttgt ggcgaatctc
     1861 agggcgcctc agagtcgcgt gtgtgattca ccacacctca acaatggact tgatccgaac
     1921 cgtgagcacc agcacctgca tgagcgtctc gatgaccgcg agtgcatgtc gaacgctgga
     1981 ggggtcatcc ggccacacgg gaatgatcag gttgggttcc ggcgtctcct ggccccagta
     2041 ttcgcattcg tcgtcgaagc acgccatgac ggcgtcgccc ggccggaagt aaagcaagac
     2101 agcgggcagc ggtagagcga atggtaatga cgctcgcgtt ccagccactc gtcgtcgagc
     2161 ctgggccgtt ccatcgagtg ggatactctg tgtagctcga ttgtggcttc gaccaactcc
     2221 ctcaaacctg ggacatcagg aactggaaac gaggtcagcg accgagccgt gttagaacgg
     2281 cgttcgtcct tcagggacgg tggcaggtcc tgctcgagct ttgggatctc gtaagactca
     2341 ggatcgtcct ctccctcggc ccactcagtc atttgctcga tgcgttcccg ggcgtccaag
     2401 tcgtcataga cacggatcca gcggtataag gcctggcgca gcccatcgta aaacgcccgt
     2461 cccatgccgg cttgggcgcc ttccagttct gtgatggcct cacccacgcc caaggcaaag
     2521 gatgactccg tattgagaac gaaaaacagt tggccggagg tggttggctc cgcctcgccg
     2581 tagatgctgt cgacgattgc ttccccgagc gtcagagtaa gttcaaagtg ttcagcaatg
     2641 gtagattggc cgcggtcggc cagataacgg cggagcacat gctctaccaa ggttgaggga
     2701 gttttcccgg cggcgatcca gtccgattca ttggcttggt cgatttccac cagagtctgg
     2761 gccaactccg taagggcgga cgaaccgaag ctcatcttat tgccggcatg aactggttcg
     2821 agccgcggaa ttccgatcag gctggcggga ttggccagat accgggtccg gggaagacgg
     2881 gatctccact tccttgctgt gagagagccc gtgcgagggc actgcttgct tcttgcgagt
     2941 ctgggcattc gagccataat gcgcagttag tttttatgcg gaggcggctt tcgaccgcct
     3001 ccggctccag ccgattgaga gactcttttg ctctgcataa tcgtggcatt tttcaactga
     3061 gctatgagtt ggtctgttgc cttgaagcga cccgtgcgca gctttggggc aatgatcgat
     3121 tcaagagttt caagcttcac ggtaggatcg gcgcgcgtgt agatttcggt tgtttgcatg
     3181 ttggcgtgtc cgagccagag agaaaccttc cgcagatcct tggtcgcctg aaggatcgtc
     3241 atcgcgcagg tgtgccgtag cacatgcgga gagactcgct tgctcgaaag cgaagggcag
     3301 ctttcttgag ccgtgcggac atgcttgcgc aagatgtatt caaagccaga acgcgtcatg
     3361 cactggttcc gtgcgttaag aaatagttct gggaccaggg cgccacccct gaccgccagc
     3421 caagctcgca acgcagagac agtctccttc caaagcggaa ggcagcgttc cttccgacct
     3481 ttgccgtgaa ccagaacatt agcttccggt tggagagtta catcattgac gtggaggccg
     3541 ataagttcgg agactcgtaa gcctccggca aagcaaaggt gcagcatcgc acgatcccgg
     3601 atgccgtccc ggacggtggg gttaggaacg tccagaatgg ctcgcaattc ctgagccgtg
     3661 aggtgcctga cgaggcgggc atctgttttc tttaccggta tggcgagaat gcgccggatc
     3721 tggtccagag ccgccggcat ccgatactcc atgaaccgca tgaaggactt gatcgccgcg
     3781 aggcgagtgt ttctcgaccc agcgctgttc atgcgggtcg tttcgaggtg atttaagaag
     3841 ttgagaacca aagcagcatc caggtgctca agttgcagtt gcgatggtga aacgttcaga
     3901 caaccgctgg catattccaa aaacagcctg aaagcgtggg cataagaatc gcaggtatta
     3961 gcactggcgc gccgctcaat gggtaaacgt tgctggaaga aggccgcgat gtgcggtgca
     4021 atgagaatca tggccgtcct ccgctgaaga actcttgggt ggaatcggca atggtcttca
     4081 ttaactgtgg cgtcgtctgc aagtaccagt aggtgttggc gaccttgccg tggccgagat
     4141 acgttgaaag tgccaacata tgttttgtga ttcggtcacg gccatcaggg caggtttcca
     4201 aagctctcac tgcaaaggtg tggcgcaagg agtggaccgt gggacgtgga agcctcggcc
     4261 cagacggcag gccgagctgc ttcactgcgc tttggaatgc cgtttcgaca tcggtgcgaa
     4321 gcaggagtct tcgccgtaac gagacgaata catgatcatc gaacggcgca tacggacgac
     4381 gtctctgaag atattcctga agcgccatcc gggctgtgtg gtgcaatgga actatgcgac
     4441 tcttgcggaa ctttgaacgt cgaatgacca gtccgtctgc ggtgatgtcg tcgtatcgaa
     4501 gaccgattgc ctcggacaca cgcagtcccg tacacgccag cagtgcaaag agcgtgctat
     4561 aggtcccacg tcggaaagaa taggaaccca gccgagatgc tgcctgcacg aggcgcctaa
     4621 tgttttcggt tgaaaagatg taagggatgg ggcgcggtcg tgtatcagag ccaaaaacgg
     4681 cgggtgggag ttcgttgcgc tggtcctcgg cccggatgta acgcgcaaag cgaattacct
     4741 ggcccaaccg atgagctcgc gtggggagcg actgcgccaa tccagcccat tggatggccg
     4801 tctgagcaca aacgtagtct ttgccgagtg cttcagagaa aactgcgaaa ctgtgtaaaa
     4861 gactgcctgt gcacttgaga gcgaatcctg tggcgcgacg cactgccaga tacgattcca
     4921 ctgcgtccac tagcatgtcg tcacctccgg ccaaggctgg gcgatctgtt gcaggacagc
     4981 aaagtccact ttggcgtaga tctccgtggc agcgatcgaa cggtgccgga gaatgacacc
     5041 gatctcctgc agagacgctc cgtgtcgcag catggaagtc gctgcggaat gtcgtagaac
     5101 atgagctgca cctcggctgg gagaactgac cccagcacga cgcatcgcgc gaccaacaat
     5161 cgccgagact gcattctgat tttgaaatgg gcgcatagga gcgcgcgagc ggataaaaag
     5221 cgcatcggta tcagcgggat ggcgagcttg gctgatgtag ccggcgagcg catcgcccac
     5281 ttcctgagtc atgggtagtc gcgtctgccg ccgccccttc ccgcagacgc agatccaggc
     5341 ttccttccag tcgatatcgc ccagccgaag ttggatgatg tcccccgctc gaagagctaa
     5401 tcgcgcaagc aacagcagaa tcgctcgatc tctcactcca acgtgagtgt tcggagcaca
     5461 cgacgcaata acccgctcga cttcgtccga ttgcaagtac cgcggtaagg aagagcaacg
     5521 ccaatgtgcc cacgtgggaa tcgccgcgtc caagccggcg gagcacttgc cctccgcgat
     5581 caaaaagcgc acgaacatcc ggacagctgt ggtacacctt ttcgctggcg accatccgtg
     5641 ccgttggctt ctttctagaa cgaacttgcg cagattctga gcatcgaact tggctggatc
     5701 ctctcccagc ttcttcagca acgcgcggag atctaggcag tagttcttaa ctgtgtcatc
     5761 gcaggtgcct cgttgctgac gcatccattc gcagaacgag acgaccagaa tcggatcgtg
     5821 ggtgaccggc tcgatgtgtg cagctgtcag cacgccggag cgtcggagat agtcgagaaa
     5881 caaacgtgcc gcatgcggca aggaccttct acgcatgtgc tcaaatccag ggcatcggca
     5941 tcgttcgata tggcgagcga acttctcaac caacggctgg tcaaacgctc ttatcggtgt
     6001 accttctcga tctgcccaat aaatgaggtg ttcgcccgct cgtatgtgtc cgcgagccgt
     6061 ttcctccgcg taaccagcta ggcaaatctc gtcagcataa cgctccagca ggcggccctc
     6121 gggttgcttc cgaatgtcct cattccgtaa ccaagattca caaaaatgtg caatcatgac
     6181 gcctctttcc atactgagtt ttggtgttgc aagacgtcac gattatgcgg tgttccaaga
     6241 agaggagatc aatcaaacgt gcctgaaaac actgaggccc ctacatgcca agaaactcaa
     6301 aacaacataa aaacgaactg cgcattatcg ctcttggact aaaccgctac gcggtaagaa
     6361 tcaggccgac gttcagttcg tagcagcggg agatccatct gggtttgact tcacgtcttg
     6421 ttccctaacg taagcctttt tgcgggacca tggagtcgct gcttgcagcg aatccatccg
     6481 acaaaggagc ttcccatgac ttctcttcgt ttgcgcatgc tcgaggacat gcagatccgc
     6541 aatttggcgg tgaacaccca agagtcgtac gtccagcagg tctcactctt cgcacgctac
     6601 ttccatcagt cacccgagcg gttggggcca gagcagattc gtgcctatca gctctacctg
     6661 acgaaagaaa agaagttggc taccggttcg atcctcattg ccatttccgc actccgcttc
     6721 ctgtataaag tcacactcaa gaagggttgg actttcgaag acatcattcc cgctccgaaa
     6781 aaacctcaga ccctgcctat ggtcctcagt cccgaggaag tcatgcaatt tctcacctgt
     6841 gtggctagca ggaaacaccg tgtcatcttg accacgtgct acgcagcagg cctgcgcgtc
     6901 tccgaatcca tcgcacttac accccccgcc atcgacagca agcgcatggt cttgcgggtg
     6961 gagcaaggca aaggaatgaa ggatcgctac gtcatgctct cccccaagct gttgcagatc
     7021 ctgcgagaat ggtggcgagt ggaaaggcct gagcactggc tcttttccgg cgatatgccg
     7081 ggccagcaca tcagccgggg cgcggttgaa ctggagtgcc agaaagcccg ccgcttgtgc
     7141 aaaatttcca aacccatcac cccgcattcc atgaggcacg ccttcgccgt gcacctcctg
     7201 gagcaaggca ccgacgtccg caccattcaa ctgctcctgg gacatcgcag cctggccacc
     7261 acagcaaaat atctccgtat tgcaaccagc aaagtgtgct ccacctcgag tccgctcgac
     7321 ttgttaccac accctattcc ccaggaggtc aagcctactc cgccaccgct cttctagccg
     7381 ctgggcccca tggatcgccc aaagctggaa gtggcggaca tcttccgccg ctatggtgaa
     7441 acctatcgac agaagcatgg ggcgtcgctc tccacgtcac agcggcgcgt catgaccgcg
     7501 atcgaggtgc gccgcacggc ggcccttggc ggtcatctcg agcgctgcga cagctgtcct
     7561 ttcgagcggc cctgctacga tagctgccgc gatcgacatt gccccaaatg ccaatcgttg
     7621 gcgcgggcgg aatggctcga aaaacggaaa gcggaaaccc tccagacaca ctattttcac
     7681 gtcgtcttca cgctccccga acaagtcgcg gctattgctt accagaacta aagatctcgt
     7741 ctacggcatt ctgttccgag ccacggccga gaccttgtcc accattgctg ccgatcccaa
     7801 acatctgggc gccgagatcg gcttcttcgc tgtactgcac agttggggac agaacc
//
LOCUS       DASRKK010000418        13518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_129774, whole genome shotgun sequence.
ACCESSION   DASRKK010000418 DASRKK010000000
VERSION     DASRKK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129774"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..552
                     /locus_tag="VFC15_09725"
     CDS             166..552
                     /locus_tag="VFC15_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10475.1"
                     /translation="MRSDRAVESYLRYGRSLANSGAAGMRSGRDAYLNGKPLSTVLTQ
                     KARASLGLAAIGACAGLLQCLVSGRDRRISKSIVLGAVGSAVGFIAGFSWKTRDLTGS
                     MVQGAAKNMGSVRDGHWLERHPIDYA"
     gene            1119..3254
                     /locus_tag="VFC15_09730"
     CDS             1119..3254
                     /locus_tag="VFC15_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZM10476.1"
                     /translation="MLDGIPSAAQATYWSIPGPVLFSVIAFLGLACFAYIVAKRLTPL
                     LRAERDVRFDRPLLRLERVFKYWLGQWKHPRFRTAGTIHILIFASFLILAFQAFSLLI
                     VGISGDAVIGRFSEAAGHAYGVIKDYAATVVFLCMVIAATRRIVFKPARYEVPAKVGK
                     AHKADAIFLLALIATLMASEGLIEASKAALQAHHGLPVGFLAVFSLPWMMNHALASTS
                     PATLWNVYRGAYLVDVLTFYFLLCYRPFGIQFHVETSLFNVYFAKLDKGTVKPVKWGL
                     TQDELDNVKSVGVKTFEDFTWKHMLDFYSCADCGRCSDQCPANAVGRPLSPRFLTTKA
                     RDYSFQHYPIFGATGNGTPLIGSIYSEDEIWSCTTCGACEEECPLLIEYIDKIVDLRR
                     GMVDDGNVPKSLQKPLKALESRGNPYGKMEKKRAEWTKSADFQASCNVPVLNGSTSAD
                     TLYFVDSITSYDDRMQSIGRATAKILDHVGTKFGILGAAEKDSGHEVRRFGEETLFMA
                     LRDDNAEAIKASGVQKIVTADPHAFNALKHDYKDMPPVEHISQLIAREVKSGKIKFNP
                     VENGEQVYTYHDPCYLGRHNHVYDEPREVLDAIPGLKRVEMERTRDRALCCGGGGLML
                     FYEPKEEQRIGVLRAKMAAEAGANVIVTACPFCMVNMEDAIKVAGLEGKLTAIDLTEL
                     VDQQIVREIPNIVATAEQSAEIKQEDALV"
     gene            3321..4112
                     /locus_tag="VFC15_09735"
     CDS             3321..4112
                     /locus_tag="VFC15_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZM10477.1"
                     /translation="MEILVCVRRVPDTAENEIALNRAGNDIERDDLVYSINEWDNYAV
                     EEALQIVAKAGGNVTVVTVGSEDDEEVLRREMAMGANHGVLITDDAFNGSDGKGIATI
                     LKAYVQKGKFDLILTGVQAADGGAQVGGMLASLLDYPFASLVNSVEVLDGKKLKISRE
                     IAGGNREMNEIDLPCVLSIQTGINEPRYVGMRGIRQVASVEIPTFGTSELGVDASSVG
                     DAAAKVKRVDYFVPALGKGAEMLQGSREENIDNLIGLLKAKGGLK"
     gene            4112..5080
                     /locus_tag="VFC15_09740"
     CDS             4112..5080
                     /locus_tag="VFC15_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZM10478.1"
                     /translation="MARIFAYIEHKGGVADDSAAELVAAAKKIDPAASPTAVVTGWGA
                     DLDAVCKSLTTSYAEVWKVANEALAYPNAELVRKALAKVVPHDSLVLVPHDHFGVDLA
                     PGLSIQMNSTFVSDVVAIEGLDGPFLKVVRQEYGGQVSTHVRCDVSSGAIVNLRPGSF
                     KPLETAPVNGTVIDKSADVGTLTSGRRYLNTVVAEAGDVDISKEAVLVSIGRGIQEKD
                     NVGIAQELADAMGAAVSCSRPVVDAKWLEKSRQVGSSGQTVKPKVYMACGISGSFQHL
                     AGLKGNPYIIAINKNPKAPIFQVADVGIVDDILEFLPELTTKIREL"
     gene            5132..6826
                     /locus_tag="VFC15_09745"
     CDS             5132..6826
                     /locus_tag="VFC15_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM10479.1"
                     /translation="MITNKTLKLSLKSLHDFAKKRLPDQELIDLDARDECPVEVVKYM
                     CNPDKLGIQLLFVPEEYGGMGGSTFDVYRICEEMARIDLGVGTSLFATFLGSDPITVG
                     ATAEQKKKWLSRIAEDGALFAYGATEPEAGSDLGSLKTTADRVMQDSKVVGYKINGSK
                     QWISNGGIADLYTVLANTPSGPSWFVVEKDAPGFTHDKPEDKHGIRLSNTAALAFNNV
                     YVDADRLIGLVEGQGLLQAQAVFGYTRLMVAAFGLGAGWSALDRAIPYSTKRIQAGSP
                     LSEKQGYTHKLMIPHITRLEAGRAFIEETAERIDAGEGSLNTEGAIAKYMSTEAGNAA
                     ADASIQALGGYGYTHEYMVEKISRDVRITTIYEGTSEIMEMTISRDRWQLHLKTRGQH
                     YHEQARLMETLHAQHSDVGADMAALALHALAEVMEKARFARLTRFQHILLRLGELIAF
                     AESAACLARRAARSAENRLNEKANNRFDATALAALARIFAREASLKVAEEGLRWVTGA
                     GGVSDSEMTAFETSLGLPAIHRAQAGLISDMDYLADVIFDRTAKKEGAPALQYANV"
     gene            7213..>13518
                     /locus_tag="VFC15_09750"
     CDS             7213..>13518
                     /locus_tag="VFC15_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZM10480.1"
                     /translation="MEDTAYRAIAIVGAGAVLPDAANVEAFWQNVKQGRYSITDVSPE
                     RWDPALYFDTDHNAPDKTYSKIGGWVREWKWEPAKWRLPIPPRVVDAMDEAQRWAIAC
                     TREALEDYGYPDRPLNTERTAVILGNAMAGEKHYFTALRIYFPEYASQLSHSASFAAL
                     PEASRRDITRELHDRIAQWIPDITEDTMPGELSNCIAGRIANVFNLRGPNYVVDAACA
                     SAMAAMSSAAEGLVANDFDVAISGGIDRNMGASTFVKFSKIGALSATGTRPYAEGADG
                     FVMGEGAVIFVLKRLADAERDGDKIYAVLRGIGGASDGKGKGITAPNPVGQKLAISRA
                     WENAGISPATVTLMEGHGTSTSVGDRVEVQSMDDVLGSFHLPVGSVALGSVKSNIGHL
                     KGAAGAAGMLKAALALRDSVLPPSVHCEHPNPNVDFAHSPLYVNTELKPWTMPADGVR
                     RAGVSAFGFGGTNFHLAMEEFIPHKLNGNGKRQVAVGDVPAAVAQGAAMYSKNVTPGV
                     TGETSRKAPLRGALVVGAATEAELVQRLSSIQQAAAAAKVPLTAAPADSDLLATERIA
                     IDYADAAELADKSAKALKAIKANQAPIWKALRPQGIFRGHGPAGKVAFLYTGQGSQYV
                     NMLAPLRAAEPIVAELFDEADRIMTPLLGKPLTDYIFVDQSNADAVAKAEEDLRQTEI
                     TQPTVLTIDLALTRMLAAYGIYPDMTMGHSLGEYGALVASGSLTFKDALEAVSARGRG
                     MTQVAVDDTGKMAAVFAPLAEVERILKTIDDYVVIANINSGQQSVIGGASKGVEQATE
                     IFLQAGYNVVPLPVSHAFHTTIVAPASGPLREMLSHLHVQSPQVPVVANVNGEFYPTG
                     PNVVPQMLDLLARQVACPVQFVKGLQTLYDAGARIFVEVGPKKALQGFAEEVLGDRGD
                     VVTFFTNHPKVGDVVSFNQALCGMYAAGLGSAEAEAKHDISVKTSASATEVNTAGPAP
                     ELAAPVMPVVVAPVVSNDAGVIQQGGAINRGQRDNSTQHPVVITGAAIGLPGTEHIFD
                     DGNIARILRGDQFIKAIPAERRKDMLDKRITRLVKSESGEARFDTIEDVADVIKLAGR
                     GGAFDLEKEFGISAERVAALDRVTRLAIAVGIDAMRDAGIPLVMRYKTTSKGTQLPDR
                     WGLPDAMRDDTGVIMGSAFPGYDSFADEMARYYADHARREQLASLENMVAAVAGTNGH
                     SALAQEIVSKIEELRAEIEKNPYVFERRFLLKTLSMGHSQFAEFIGARGPNTQINSAC
                     ATTTQAVGMAGDWIRAGRCSRVIIITADDVTSDHMIGWFGAGFLASGAAATDEKVEEA
                     ATPFDRRRHGLIMGMGAASLVVESADAARERGIQPICEVLSAETANSAFHGTRLDVNH
                     IGQIMENLVAHAEAHSGIQRQQIAPHTVFVSHETYTPARGGSASAEINALRRVFGSTA
                     DQVVIANTKGLTGHAMATGIEDVLAVKTLETGTVPPVANFKEIDPELGSLNLSKGGTY
                     PIEYSLRLGAGFGSQISMSLMHWVPTKDGVHHSANDLGYAYRIVNKPAWDAWLTRMAG
                     RPAADLEVVNRTLRVKDQGLSARVAEVAKEVKLVAAPTPTIQAPPPAPKPAVVAAKPA
                     PTPPPSVPAKTSPQVQAVAVASATKPKAAVTNDSVKERVLALVAEKTGYPIDMLDLDL
                     DLEADLGVDTVKQAEVFATIREAYGIARNDQIKLRDYPTIAHVIRFVNEHRPDAAVAV
                     QPTAETPTEKTAVKTTPSAPAAVVAPVSAGDAVKERLLDLAVEKTGYPRDMLDLDLDL
                     EADLGVDTVKQAEMFAAIREIYSIPRDENRKLRDYPTLAHVMRFVYESRPDLAPGVSP
                     APVTVAVAAATKPTVTATVAAPAPVTAVQAVADGEAVTERILTLMAEKTGYPKDMLDL
                     ELDLEADLGVDTVKQAEMFAAVREIYNIPRDENRKLRDYPTLAHVIRFVYESRPDLAP
                     GVAPKPATVAAAAAPTPTVPATVAAPVTAVQAVAEGESVTERILTLMAEKTGYPKDML
                     DLELDLEADLGVDTVKQAEMFAAVREIYNIPRDENRKLRDYPTLAHVIRFVYESRPDL
                     APGVTPKAATVEVAAATAPTVAATVAAP"
BASE COUNT         2915 a         3901 c         4100 g         2602 t
ORIGIN      
        1 accggcggtt gtcaaactgg gactccagca ggatgtggcg taaaattaaa ttttggcgaa
       61 aatcacccga gtgggtgcgg tctgtcacat cgtcgatgta caaatcggat gtaagaagaa
      121 actaaggcga agctttcagg atggagcttc taaggagcac ttcgtatgcg atcggataga
      181 gcggtcgagt cctatttgcg ttatggtcga tctctggcca actcaggcgc agccggaatg
      241 cgcagcggcc gcgatgccta tctcaacggc aagcctctca gcacggtcct gacgcaaaag
      301 gcgcgtgcat ctctgggact ggccgccatc ggagcctgcg ccggcttgct gcaatgtctc
      361 gtgtctggcc gcgatcgacg tatttcgaag agcattgtgc tgggagccgt gggcagcgca
      421 gttggattca tcgcgggatt ttcctggaag actcgcgacc tcacgggaag catggtacag
      481 ggcgcggcca aaaacatggg ttcggttcgc gacggacatt ggctcgaacg tcatccaatc
      541 gactacgcct agttctggaa ttaacagaca accaccgagg cagcgctccc gcgctgcttt
      601 gttatttcaa acatcgtttc ggccttgacg cagccatcca atcggagcgt caaaggttca
      661 ttgccttccg ttcagcttgc cgcgagtcgt cgttgtcgct agcggtttgt gcgcttttga
      721 cgggtgcact gtgatctgcc tcacagactg tgcgcccgta gcgacgaaat aatcaaccat
      781 gtcagcgggg cagcgatagc gacgtggcat cgatggtcct cctgttgtca gcgatcatct
      841 tcgatgcccg gtagatgtgg ccgcaaatac ctgagcaacc catctgactg ggacccgaca
      901 gtaaacagcg ttcgacgcag gcagcaatcc ataccaaact catgggagtt tcgctgtgaa
      961 ctgagggggc accgtgcagc ctgcattcgc ggctgaccgg ctgccgaaag tgcttgtccg
     1021 cttccgtcat ccggagtgaa gcgaatggct ggcagtattt ttctgcagcc tacgacttgg
     1081 cagtgaattg agtttgtgtt tcagaaaaga gtatccgtat gttggacggt atcccctcag
     1141 cggcgcaagc gacctactgg tcgatccccg ggccagtttt gttttcagta attgcattcc
     1201 tgggcctcgc atgctttgcc tatattgtgg cgaagcggtt gactccgctc cttcgtgccg
     1261 agcgcgatgt tcgtttcgat cgcccactcc tccggctgga aagagttttc aagtattggc
     1321 tgggacagtg gaagcatccg cgtttcagga ccgcggggac cattcacatt ttgatttttg
     1381 cgagtttcct gatcctggcg ttccaagctt tttcgctcct tatagtaggg atctccggcg
     1441 atgccgtgat cgggcgcttc tcggaagctg cgggccatgc gtacggcgtc atcaaggact
     1501 acgccgccac ggttgtcttc ctgtgcatgg tgatcgccgc gactcgtcgc atcgtcttca
     1561 agcccgcgcg ctatgaagtg ccggcaaagg tcggcaaggc ccataaggcc gacgccattt
     1621 tcctgttggc gctgatcgcg acgctgatgg cgtctgaggg cctaattgaa gccagcaaag
     1681 cggcgctgca agcccaccac ggactacccg tgggattcct ggctgtcttc tcgctgccat
     1741 ggatgatgaa ccatgctctg gcatcgacct cccccgcgac cctctggaat gtttatcgcg
     1801 gcgcctatct ggtcgatgtg ctgacgttct atttcctgct ctgctaccgt ccgtttggaa
     1861 ttcagttcca cgtcgagact tcgctgttca acgtttactt cgcgaaactg gataaaggca
     1921 cggtcaagcc ggtcaagtgg ggcctcaccc aggacgagtt ggacaacgtg aaatcggtcg
     1981 gcgtgaagac gtttgaagac ttcacctgga agcacatgct cgatttctat tcctgcgccg
     2041 actgcggccg gtgctcggac cagtgtccag ccaacgccgt gggaaggccg ctgtcgcccc
     2101 ggttcctcac tacgaaggcg agagattaca gcttccagca ttatccgatt tttggcgcca
     2161 ccggcaacgg tacgccactc attggcagca tttattccga ggacgaaatc tggtcgtgca
     2221 ccacctgcgg cgcttgcgaa gaggagtgcc cgctgctgat cgaatacatc gacaaaattg
     2281 ttgacttgcg gcgcggcatg gtcgacgacg gaaacgtgcc gaagtccctg caaaaaccgc
     2341 tcaaggcact ggagagccga ggtaatcctt acggaaagat ggagaagaaa cgggcggagt
     2401 ggaccaagtc cgcagacttc caggcgagct gtaacgtgcc tgttctgaac ggcagcacga
     2461 gcgccgacac cctgtatttc gtcgacagca tcacttctta cgacgacaga atgcagtcga
     2521 ttggacgtgc caccgccaag attctcgatc atgtcggaac gaaatttggg atcctcggcg
     2581 cggcagaaaa agacagcggg catgaagtcc gccgcttcgg cgaagagacc ttgttcatgg
     2641 cgctgcgcga cgataacgcg gaagccatca aagcctcggg tgtgcagaag atcgtcactg
     2701 ccgatccgca cgctttcaac gcgctgaagc acgactataa agatatgccg ccggtggaac
     2761 acatcagcca attgatcgcc cgggaagtga aatccggcaa gatcaagttc aatccggtag
     2821 aaaatggcga gcaggtttac acctaccacg acccgtgcta tctggggcgc cacaatcacg
     2881 tgtacgacga accgcgcgaa gtgctcgacg caattccggg cctgaagcgc gtcgagatgg
     2941 agcgcacgcg ggaccgcgct ctgtgctgcg gtggcggcgg gctgatgttg ttctacgagc
     3001 caaaagaaga gcagcgaatc ggtgtgctgc gggccaagat ggccgccgaa gccggcgcca
     3061 atgtgatcgt gaccgcctgc ccgttctgca tggtgaatat ggaagatgcg atcaaggtcg
     3121 caggcttgga aggcaagttg accgcgatcg atctaacgga gttggtggac cagcaaatcg
     3181 tacgtgaaat tccgaatatc gtcgcgacag cagaacagag cgccgaaatc aaacaagaag
     3241 atgcactggt ctagttcgac agatggaatg atttcgcatt ggaagtggat ttcaggactg
     3301 agaactcttg ggagatgaat gtggaaattc tagtttgcgt aagaagagta cccgacacgg
     3361 cggaaaatga gattgccttg aaccgtgccg gaaacgacat tgaacgcgac gacctcgtgt
     3421 attcgatcaa tgagtgggac aactacgccg tcgaagaagc cctgcagatc gtcgcgaagg
     3481 ctggtggcaa cgttacggtg gtcacggttg gcagcgaaga tgacgaagaa gttctgcgcc
     3541 gggagatggc catgggcgcc aaccatggcg tgctcatcac cgatgacgcg ttcaacggat
     3601 cggacggcaa gggaatcgca accatcctga aagcctacgt gcagaagggc aagttcgacc
     3661 tgatcttgac cggagtgcag gctgcggacg gaggcgcgca ggtcggtggc atgctggcct
     3721 ccctgctcga ttatcccttc gcttcgctgg tgaactccgt cgaagttctg gatggcaaga
     3781 agttgaagat cagccgcgag atcgcaggcg gcaacagaga aatgaacgag atcgatctcc
     3841 cctgcgtgct ctcgatccag actggcatca acgaaccgcg ttacgtcgga atgcgaggca
     3901 tccggcaagt ggcctcggtg gagattccga ctttcggtac atcggagttg ggagttgacg
     3961 cgagttccgt cggcgacgca gccgcgaaag tgaaacgagt ggactacttc gtgcccgctc
     4021 tgggtaaggg cgctgagatg ctgcagggca gccgggaaga aaatatcgac aacctgattg
     4081 ggttactgaa agccaaggga ggacttaaat aatggctcga atttttgcct acattgaaca
     4141 caaaggcggc gtggccgacg attccgcggc cgagttggtg gctgcggcaa aaaagatcga
     4201 tcccgcagca tctcccacag cggttgtcac cggttggggt gcggacctcg atgcggtttg
     4261 caagagcctc accacttcct acgcagaagt atggaaggtt gccaacgaag cactggccta
     4321 ccccaacgcc gaactggttc gcaaggcgct cgccaaagtg gtgccgcacg acagtctcgt
     4381 gctggttccc catgaccatt tcggagtcga tcttgctccc ggcctttcga tccagatgaa
     4441 ctcaacattc gtgtccgacg tagtggcgat cgaagggctg gatggaccgt ttctcaaagt
     4501 cgtgcgccag gaatacggcg gacaggtgag cacgcacgtg cgctgcgacg tttcctcggg
     4561 tgcgatcgtc aaccttcggc ccggctcatt caagcccctg gagactgcgc cggtcaacgg
     4621 gacggtgatc gacaagtcag cggatgtcgg aactcttacc tccggtcgcc gttatctcaa
     4681 tacggttgtg gccgaggcgg gagatgttga catcagtaag gaggccgttc tggtttcgat
     4741 tggccgcggc atccaggaaa aagacaacgt tggtatcgcg caggaactgg ccgatgccat
     4801 gggtgcagcg gtgagttgct cgaggcccgt ggttgacgcc aagtggctgg agaaatcccg
     4861 gcaagttggt tcatccggac aaaccgtgaa gcccaaggtt tacatggcat gcggcatcag
     4921 cggttccttc cagcacctgg ccggactcaa ggggaatccc tacattatcg ccatcaacaa
     4981 gaaccccaag gccccaatat tccaggtggc cgacgtcgga atcgtggacg acatcctgga
     5041 gtttttaccg gaactaacca ccaagattcg tgagctgtga caccggaaag ttattcaatt
     5101 ctcggagatg agtgaaagcg aggcaccgcc aatgatcact aataagaccc tgaaattaag
     5161 cttgaagtcg ttacacgatt tcgccaagaa acgcttgccg gaccaggaac taatcgatct
     5221 ggacgcgcgc gatgaatgcc cggttgaagt tgttaagtac atgtgcaacc cggataaatt
     5281 gggcatccaa ctgctgttcg ttcccgagga atacggcggg atgggcggaa gtaccttcga
     5341 tgtgtaccgt atctgcgaag aaatggcccg catcgatctg ggcgtaggca cgtccttatt
     5401 tgcgacattt cttggcagcg acccgattac ggtcggcgcc acggcggagc agaagaagaa
     5461 gtggcttagc cgcattgccg aggacggcgc gctgtttgcc tacggcgcta ctgagccgga
     5521 agccggcagc gacctgggtt cactaaagac caccgcagac cgcgtcatgc aggacagcaa
     5581 agtggtcggg tacaagatca atggcagcaa gcagtggatc agcaatggcg gaatcgccga
     5641 cctttacacg gtcctggcga ataccccgag cgggccgagt tggttcgtcg ttgaaaaaga
     5701 cgcgccggga ttcacccatg acaagccgga agacaagcac ggcatccgcc tcagcaacac
     5761 ggccgcgctc gcgttcaaca atgtttacgt cgatgccgac cggctgatcg gactcgtgga
     5821 agggcaggga ctgctgcagg cacaggcagt gttcggctac acgcggctca tggtggcggc
     5881 gtttggtctt ggggccggat ggtccgcgct ggatcgggcg attccgtatt caaccaagcg
     5941 catccaggct ggttcgccgt tgtcggaaaa gcagggctac acccacaagc tgatgatccc
     6001 gcacattacc cgtctggaag ccggtcgcgc tttcatcgaa gaaaccgcag agcgaattga
     6061 cgcaggtgag ggaagcttga acaccgaagg cgccatcgcg aagtatatgt cgacagaagc
     6121 gggcaacgca gctgccgacg ccagtatcca ggcgcttggt ggctatggct acacgcatga
     6181 gtacatggtg gagaaaatca gccgcgacgt tcgcatcacc accatctacg aaggcacctc
     6241 cgagatcatg gagatgacga tcagtcgcga tcgctggcaa ttgcacctga agacgcgcgg
     6301 acagcactat cacgagcagg cgcgcctgat ggaaactttg cacgcccagc actccgatgt
     6361 cggtgccgac atggcggcgc ttgccctgca cgcactggct gaggtgatgg agaaagcacg
     6421 cttcgctcgg ctgacgcgtt ttcagcacat cctgcttcgc ctgggcgagt tgatcgcttt
     6481 cgcggagtct gcggcctgtt tagcccgtcg tgctgcgcgc tcggcggaaa accggctcaa
     6541 cgaaaaagcc aacaatcgct tcgacgcgac cgcgctggcg gcgctggccc gtatcttcgc
     6601 gcgcgaggcc tcgcttaaag ttgcggaaga aggtctgcgc tgggtaaccg gtgcgggcgg
     6661 tgtgagcgac tcggaaatga ccgcgttcga aaccagcttg ggactgcccg cgatccatcg
     6721 cgcgcaggcc ggtttgattt cggacatgga ttacctcgcc gatgtgatct tcgaccgcac
     6781 ggcgaaaaaa gaaggggctc ccgccttaca gtacgcgaac gtttaagcga atgacggcag
     6841 gcatccggct atgcactttt gcgtgccgga tgccggtccc gttaatgccc ggatgtccgg
     6901 gtgattttgg ggtcgaggtg acaatgggct ggcctgggaa agaccgctgc cgtctcgaac
     6961 gtggtggtcg ctaccccagt ttgtgcgctg cgtcacaggg caagggctgc gatatcactg
     7021 acactgacac cttgctcccc tcaaagttgt attactctcg gccgcccagg atttgcttgg
     7081 ccttgggagt gaaacactcc ccctcgtttc ggcgaaggaa acaagacctg gcataaccaa
     7141 tgtccttttc ctgtgcgctt ggagccacgc cccgagtggg gtccgaatac gcttcaaagg
     7201 aagtgactca ccatggaaga taccgcctat cgcgcgatcg caattgtggg agcaggagcc
     7261 gtcctgcccg atgccgccaa cgtagaagca ttttggcaaa acgtgaagca agggcgctac
     7321 agcatcaccg atgtttcgcc cgagcgttgg gacccggcgc tgtacttcga taccgaccat
     7381 aacgcgcccg acaagacgta ttcgaaaatt ggcggctggg tgcgcgagtg gaaatgggag
     7441 cctgcgaaat ggcggctgcc catcccgccc cgcgtggtcg atgccatgga tgaggcgcag
     7501 cgctgggcca ttgcctgcac ccgcgaagcg ctggaagatt acggctaccc tgaccgaccg
     7561 ctgaacaccg aacgcacggc ggtaattctt ggcaacgcca tggccgggga aaagcattat
     7621 ttcaccgcgc tgcgcatcta ctttccggaa tacgccagcc aactgtcgca ctctgccagt
     7681 tttgctgcac tgcccgaggc gtcacgccgc gacatcacgc gcgaactgca cgatcgcatc
     7741 gcccaatgga ttcccgacat cacggaagac accatgccgg gcgaattgtc gaactgcatt
     7801 gccgggcgca tcgccaacgt ttttaactta cgcggaccca actacgtcgt agatgcggcg
     7861 tgcgcatccg cgatggcggc aatgagctcc gcagccgagg gactcgtagc caacgatttt
     7921 gacgtggcga tctccggcgg catcgatcgc aacatgggcg cctcgacctt cgtcaagttc
     7981 agcaagatcg gagcgctgtc ggcgaccggc actcgcccct acgccgaagg agccgatggc
     8041 ttcgtcatgg gcgaaggcgc ggtgattttt gtgctgaaac ggctggctga cgcggagcgc
     8101 gacggcgata agatctacgc cgtgttgcgc ggaatcggcg gggccagcga cggcaagggc
     8161 aagggcatta cggcgcctaa tccggtcggc cagaaattgg ctatttcgcg cgcctgggag
     8221 aatgccggta tatcgcccgc gactgtgaca ttaatggaag gccacggtac atccaccagt
     8281 gtgggcgacc gggtcgaagt gcagagcatg gatgatgtgc tgggaagttt ccaccttccc
     8341 gtcggttcgg tagcgctcgg ctcggttaaa tcgaatatcg gccacctcaa aggggctgcc
     8401 ggggccgccg gaatgttgaa ggccgccctg gcgctgcgcg acagcgtgct gccacccagc
     8461 gtgcattgcg agcatcccaa cccgaatgtt gattttgctc actcgccgct ctacgtcaac
     8521 accgaactga aaccgtggac catgcccgct gatggcgtcc gacgcgccgg agtaagcgcg
     8581 tttggcttcg gtggcaccaa cttccatctt gcgatggaag aattcattcc ccacaagctc
     8641 aatggcaacg gcaagcgaca ggttgcggtc ggtgatgttc cggcggcggt cgcacaggga
     8701 gcggcaatgt attcgaagaa tgtcacgcct ggcgttacgg gtgaaacttc acgcaaagca
     8761 cctttgcgtg gcgcgttagt ggttggcgcc gccaccgaag ccgaactggt ccagcgtctg
     8821 agttcgatcc agcaagccgc tgcggcggcc aaagtacctt taacggctgc gccggctgat
     8881 tccgatctgc tagcgaccga acgcatcgcc atcgactacg ccgatgccgc cgaattggcg
     8941 gacaagtccg ccaaggcgct gaaagcgata aaagccaacc aggctccgat ctggaaggcg
     9001 ttgcgtcccc aaggcatctt ccgtggacac ggccctgccg gcaaagtggc gttcctgtac
     9061 accgggcaag gctcgcagta cgtcaacatg cttgcgccac tgcgcgccgc cgaaccaatc
     9121 gtggccgaac tgttcgacga agcggaccgc atcatgactc cgctgctcgg caagccgctg
     9181 accgactata tcttcgttga tcaaagcaac gcggacgccg tcgccaaggc ggaagaagat
     9241 ctgcgccaga ccgagatcac tcagcccacc gtgctgacca tcgacctggc tctcacccgg
     9301 atgttggccg cctacggcat ctatcccgac atgacgatgg gacacagcct gggcgaatac
     9361 ggcgcgctgg ttgcgtccgg ctccctcacc ttcaaggacg cattggaagc agttagtgcc
     9421 cgtggccgcg gcatgaccca ggtcgcagtg gacgataccg gcaaaatggc agcagtgttt
     9481 gcgccactcg ccgaagtcga gagaatcctg aagaccatcg acgactatgt tgtgattgcc
     9541 aacatcaaca gcgggcagca gtcggtcatc ggcggcgcca gcaaaggcgt tgaacaagcc
     9601 acggagatct tcctgcaagc cggctacaac gtagtgccgt tgccggtcag ccacgctttc
     9661 catacgacga tagtggcccc agccagcggg cccctgcgtg agatgctgtc acacctgcat
     9721 gtgcagtcgc cccaagtacc ggtggtcgcc aatgtgaacg gcgagtttta tcccacggga
     9781 cccaatgttg ttccgcagat gctggacctg ctggcgcggc aagtcgcctg tcccgtgcag
     9841 ttcgtgaagg gactgcagac gctctacgat gcgggcgcgc gcattttcgt cgaagtcggg
     9901 ccgaagaagg cgttgcaggg atttgccgag gaagtgctcg gcgatcgcgg cgacgtggtt
     9961 acgttcttca ccaatcatcc gaaagttggc gatgttgtga gtttcaatca agcgctgtgc
    10021 ggaatgtatg cggcgggatt gggaagtgca gaggcggaag ccaagcacga catctccgtg
    10081 aagacttctg catcagcgac tgaagtcaac acggcaggac ctgcgccaga acttgcagct
    10141 cccgtaatgc cagttgttgt tgcacctgtg gtatcgaacg atgccggtgt aatccaacaa
    10201 ggcggagcga ttaaccgtgg tcagagagac aactctaccc agcatccggt ggtgattacc
    10261 ggcgcggcga ttggtcttcc gggaaccgag cacatcttcg acgatggcaa catcgcacgc
    10321 attctgcgcg gcgatcagtt catcaaggcg atccccgctg agcgtcgcaa agacatgctc
    10381 gacaaacgca tcacgcggct ggtgaagagc gagagcggag aggcgaggtt tgacaccata
    10441 gaggatgtcg ccgacgtcat caagctggcg ggccgcggtg gtgccttcga tctcgaaaaa
    10501 gaattcggca tttccgccga gcgcgtggcc gcgctggatc gggtcacccg cctggcaatc
    10561 gccgtgggca tcgacgcgat gcgcgacgct ggaattcctc tggtcatgcg ctacaagacc
    10621 acaagcaagg gcacgcagtt gccggaccgt tggggcttgc ccgacgcgat gcgcgacgac
    10681 accggcgtga ttatgggctc ggccttcccg ggatacgatt ccttcgccga cgagatggcg
    10741 cgctattatg ccgaccatgc ccgccgcgag caattagctt cgctggagaa catggtcgca
    10801 gcggtggcgg ggacgaacgg tcattccgcc ctcgctcagg aaattgtcag taagatcgaa
    10861 gaattgcgcg cggagattga gaagaacccg tacgtcttcg agcggcgctt cctgctcaag
    10921 accttgtcga tgggacattc gcaattcgcc gaatttatcg gcgctcgcgg tcccaacacg
    10981 caaatcaact ccgcttgcgc gaccaccacg caggcggttg gaatggcggg ggactggatc
    11041 cgcgccgggc gttgcagccg ggtgatcatc atcacggcag acgacgtcac atccgaccat
    11101 atgatcggat ggtttggcgc cggcttcctg gccagcggtg cggccgctac agatgaaaaa
    11161 gtagaagagg ccgccacacc ctttgatcgc agacgtcacg gcctgatcat gggcatgggc
    11221 gcagcgtcgc tggtagtcga gagcgcagac gccgcgcgcg aacgcggcat ccagcccatc
    11281 tgtgaagtgc tgagcgcgga aaccgctaat agcgctttcc atggtacgcg tctcgatgtc
    11341 aaccacatcg gccagatcat ggagaacctg gtcgcgcacg ccgaagcgca cagcggcatt
    11401 caacgtcagc agatcgcgcc gcacaccgtc ttcgtctccc acgagacgta cacgcccgcg
    11461 cgcggcggaa gcgcttccgc agagatcaac gctctgcgtc gtgtcttcgg cagcaccgcc
    11521 gaccaagtcg tcatcgccaa caccaagggt ctcaccggcc acgccatggc tacgggaatc
    11581 gaggacgtgc tggcggttaa gacactggaa acgggaaccg tcccgccagt ggccaacttc
    11641 aaggaaatcg atcctgaact gggctcattg aacctctcga agggtggcac ctatcctatc
    11701 gagtactcgt tgcggctcgg agccggattc ggttcgcaga tcagtatgtc gctgatgcat
    11761 tgggtcccga ccaaggacgg agttcatcac agcgcgaacg atctgggtta cgcctatcgc
    11821 atcgtcaata agcctgcgtg ggacgcatgg ctgaccagga tggcgggacg tccagccgcc
    11881 gacttggaag tggtgaaccg caccctgcgg gtgaaagatc aaggtctgtc cgcgcgggtt
    11941 gccgaggtcg caaaggaagt caaactagtg gcggctccga cgcccacgat ccaggcaccg
    12001 ccgcctgcgc cgaaaccggc agtcgtcgct gccaagccgg caccaactcc gccgccgagt
    12061 gtgcccgcca agacgagccc gcaagtccag gcagttgcgg tcgcgtcggc tacgaagcca
    12121 aaagctgccg taaccaacga ttcagtgaaa gagagagttc tggcgctggt cgccgagaaa
    12181 accggctatc ccatcgacat gctcgatctc gaccttgatc tcgaagccga tttgggtgtc
    12241 gatacggtca agcaggcgga ggtatttgcg acgatccgcg aagcctacgg catcgcccgt
    12301 aacgatcaga tcaaattgcg cgactacccc accattgctc acgtaatccg cttcgtgaac
    12361 gagcaccgcc cggatgccgc cgtcgcagtg cagcccaccg cggaaacgcc cacagagaag
    12421 acagccgtca aaactactcc atcggcacct gcagcagtcg ttgcgccagt gagtgccggc
    12481 gatgctgtga aagagcggct tttggacctg gcagtcgaaa agaccggcta tcccagagac
    12541 atgctggacc tggatctcga cctcgaagcc gaccttggcg tcgatactgt caagcaggcg
    12601 gaaatgtttg cagctatccg cgagatttac agcataccgc gggatgagaa ccgcaaactg
    12661 cgtgattacc cgacgctggc tcatgtgatg cggttcgtgt acgagagtcg tcccgacctg
    12721 gcgccgggcg tttcgcccgc gccggtaacg gttgcggtgg ccgctgccac caagccaact
    12781 gtcaccgcaa ccgtggccgc acccgcaccc gtcacagcgg ttcaggctgt cgccgacggc
    12841 gaggcagtca cggaacgcat cctgacgttg atggcggaga agaccggcta tccgaaagac
    12901 atgctggact tggaactgga tctcgaagcc gacctcggcg tggatacggt caaacaggcg
    12961 gagatgttcg ccgccgtccg cgagatctac aacattccgc gggacgagaa ccgcaagctg
    13021 cgggactatc cgactctggc tcatgtaatc cggtttgtgt acgaaagccg tcccgacctt
    13081 gcgccgggcg ttgcgcctaa gcccgcaacc gttgcggcag ccgctgcacc tacgccaacc
    13141 gtgcccgcaa ctgtggccgc acccgtcaca gcggttcagg ctgtcgccga gggcgagtca
    13201 gtcaccgaac gcattctgac cttgatggcg gagaagaccg ggtatccgaa agacatgctg
    13261 gacttggaac tggatctgga agccgacctc ggcgtggata cggtcaaaca ggcggagatg
    13321 ttcgcggctg tccgcgagat ctataacatt ccgcgcgatg agaaccgcaa actgcgggac
    13381 tatccgactc tggctcatgt aatccggttt gtgtatgaaa gccgtcccga ccttgcgccg
    13441 ggcgtcacgc ctaaggccgc aaccgttgag gtggccgctg ctactgcgcc aactgtcgcc
    13501 gcaacagtgg ccgcgcct
//
LOCUS       DASRKK010000419         8330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1010326, whole genome shotgun sequence.
ACCESSION   DASRKK010000419 DASRKK010000000
VERSION     DASRKK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010326"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFC15_09755"
     CDS             <1..989
                     /locus_tag="VFC15_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZM10481.1"
                     /translation="VAENVVRIEAEALRALADRIAGPMARDFERAIELLYRCAGRVVV
                     TGMGKSGLIARKVAATLSSTGTPALYLHPAEALHGDLGMIVRGDAVVAMSASGETGEI
                     LQLLATVKRLGVPLITLTCDDLYSPAAIDRRRSTLVQAADVALDCSVANEACSFGLAP
                     TASTTTMLALGDALAVALSQKRGFKEEDFANLHPGGKLGKRLTRVSALMHGGDALPGV
                     TPSTAITQVIYEMSRKGLGMTTVIEDGKLVGVISDGDLRRLLERHGKEVFDLTASQCM
                     TREPKTIGPDAFAIAALDIMEQKKITSLPVVDDTGYLLGVLHLHDLWGTQMV"
     gene            complement(1021..2259)
                     /locus_tag="VFC15_09760"
     CDS             complement(1021..2259)
                     /locus_tag="VFC15_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM10482.1"
                     /translation="MDLARIFGVPAALLVIAVIANRLSRWTRVPDIIVLLLIGVGLGP
                     ILHWVDPSNFSVVIRILGMLALILILFEGGLELRLKETVRYSPGAILLAFVSYSCTVG
                     LIASVARVLLHLTWMDCALLGAVLGSTSAAVVLPAIQQIAAPEAIKITLTLEASLGEI
                     IAVLTVGTLIGLDGNQPIFQGLITGFGHHVLVDVVLGVAAGVAWSRVWPQFASQQFSN
                     ALNLGMVLGVFAAGRYAGGSGLLAELVFGLTLANMPRTPHTTRQGARMMAFHSELAFL
                     VRSFFFVLLGIMAQFVSRAYVVPILGILVALVLARYVAVRATRWSIRDVQPADSELLF
                     LMMPRGLITAVLALQVLAARGQVFFFLPAMAFTVVLFTNIFVVVAAVTAKKAEAGVAI
                     PVVEASDVGAVPSVEPEALT"
     gene            complement(2377..2778)
                     /locus_tag="VFC15_09765"
     CDS             complement(2377..2778)
                     /locus_tag="VFC15_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZM10483.1"
                     /translation="MHRLTIKTNRKREILDITDRVETLLSDHYGGQLGVCHLVVLHTT
                     AALATADLDPGTDTDMLDAFERIIPKLKYRHPHDPEHVPDHILSAIIGTSVGLLAEKG
                     SLVLGTWQRVILIELDGPRQREIAVGFFVGV"
     gene            2920..4593
                     /locus_tag="VFC15_09770"
     CDS             2920..4593
                     /locus_tag="VFC15_09770"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM10484.1"
                     /translation="MPAENPDHELAANSRRGFLKASGVALAGTAFLPGLLSSQPNLAY
                     PANDAVPHFELDETDLATLQQKLKSGEYSSKKLVELYRSRIETIDRHGPALNAVIEIN
                     SDATAIAQQMDAERKAKGPRGPLHGIPILIKDNIATADKMQTTAGSLALVGSVPPHDS
                     AVAAKLRAAGAVILGKSNLSEWANLRSSHSTSGWSGRGGLTKNPYALDRNPSGSSSGS
                     GTAVASSLCAVAVGSETDGSIVSPSNNCGIVGIKPTVGLISRTGIIPISHTQDTAGPM
                     TRTVADAAALLSVLAGVDSQDPATAAAGEHIQLDYTKFLDKNGLRGARIGVVRKYAGF
                     NTDVDSLFDEAIEAMKHAGAEVVDPVEIPTIGKFDDAELLVLLYEFKDDLNRYLAWLG
                     ASTQVHSMKDVIKFNDDHRDQEMPYFGQDLMLQSEAKGPLTSPEYLKALNDCRRQSRT
                     EGIDAAMDKFKLDALIAPTGGPAWTTDLLNGDHTVGGSSTLAAVAGYPNVNVPMGFTF
                     GMPVGVSFFGRAWSEPVLIKLAYAYEQLTNARKLPNFSPSADLSLKSQK"
     gene            complement(4653..5726)
                     /locus_tag="VFC15_09775"
     CDS             complement(4653..5726)
                     /locus_tag="VFC15_09775"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HZM10485.1"
                     /translation="MIDLSKIREVPHRRFNPLTREWVLVSPHRTQRPWQGQTERQPEE
                     REFRFDPHCYLCPGNERAGGVRNPQYDSTFVFDNDFAALKPDVPNDDVSDGAQGLLVA
                     QTETGVCRVLCFSPRHDMTLAQMDVSSIRRVVDLWTDQYRELGAMREINHVQIFENRG
                     EMMGCSNPHPHGQVWADQTIPNVPRLEQESLTEYRHRNGSCLLCDYVELERAAGERIV
                     CENEHFLALVPFWAVWPFEILLCSKSHVADLCGLDDRARTALADLLKRITTRYDNLFE
                     APFPYTMGFHQQPTDGVEHPEWHLHAHFFPPLLRSATVRKFMVGYELLATPQRDITAE
                     SAAERLRNLPEQHYRNGNAHAGD"
     gene            complement(5723..6919)
                     /locus_tag="VFC15_09780"
     CDS             complement(5723..6919)
                     /locus_tag="VFC15_09780"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZM10486.1"
                     /translation="MEQAEVADNSRPTMRLAAQTFKKLYGEPIGIFRAPGRVNLIGEH
                     TDYNDGFVMPAALEFYTYVAAGPRIDRTLSVYSIDFDETRNFDLGHLAGGPTGHWSDY
                     VRGVAAVLQASGNSIRGANLVIKGEVPIGAGLSSSAAIEVASALALMTNSELEWSRVQ
                     VAKACQRAEHEYAGTKCGIMDQFISCCGRANHALLLDCRSLDYDLLRIDDRVRIVVCN
                     TMVKHELAGGEYNRRRADCEAGVSYLRRQLPEIQTLRDVSLPQLKEYGAGLSDVTYRR
                     CRHVISENARVLEAAQALRDGKLARFGALMGESHVSLREDYEVSCKELNVMVDLARKC
                     HGVYGARMTGGGFGGCTVNLVDVGAVEEFKATIAREYERAMGLHPEIYVCTAADGAGE
                     ITNLMA"
     gene            complement(7120..>8330)
                     /locus_tag="VFC15_09785"
     CDS             complement(7120..>8330)
                     /locus_tag="VFC15_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10487.1"
                     /translation="RGKLGCIMRKGFQLIEAVLVIVVFLLGSASAQQGGAAGSKTVQP
                     KAPTRVTPTPTVPPQTFYYSGKVVMDTGMAPPSPVAILRICNSSSRRETFTSGDGGFS
                     FVVGDRAPSMMPDASEDTRSFGSDSQTSRNDPMTTGQNNFSSPTADCELRADLSGYSS
                     SSIRLDASMNNTNVGVIVLHSRGKKVEGMVSVASMQVPAKARKEYEKGSEALEKGSLA
                     DAEKLLRKAIEEYPKFAEAWLRLGDLEQRRKNMEGATSDFQEAINADPNFALPYLRMA
                     FLSAVASNWEQTHKLTERLLALDPINFPLGYYYNAVAEFNLNHLPQAETNALRAESLD
                     KLHSEPRVELLLASIYTAKGAPAAAADHYRAYLKLVPDGPLNERVKADLAKTEAMANS
                     RGATASPVNK"
BASE COUNT         1790 a         2539 c         2314 g         1687 t
ORIGIN      
        1 atgtcgcaga aaacgttgtg cgcatcgagg ctgaagcgct gcgtgctctc gccgatagaa
       61 tcgccggccc catggcgcgt gacttcgagc gcgccatcga gttgctgtat cgctgcgctg
      121 gtcgggtggt tgtcaccggt atgggcaaga gtggactcat cgcccgaaaa gtagccgcaa
      181 ctctcagttc caccggcact cctgcgcttt acctgcatcc cgccgaagcc ctgcatggcg
      241 acctcggaat gatcgtgcgc ggcgacgccg tggtagccat gtcggccagc ggtgaaactg
      301 gcgagatact gcaattgctg gccacggtga aacgactggg agtcccgctg atcacgctca
      361 cttgcgacga cctatactca cccgcggcta ttgaccgcag acgctccacg ctggtccagg
      421 ccgcagacgt ggcgctcgat tgctccgtag cgaatgaagc ctgttcgttc ggattggccc
      481 ccacggcatc gaccacgacc atgctcgctc taggcgatgc cctggcggtc gcactctcgc
      541 aaaaacgcgg attcaaagaa gaagacttcg ccaaccttca tcccggcggc aaactgggca
      601 agcgactcac tcgggtatcc gctctaatgc acgggggcga tgcattgcct ggggtcactc
      661 cttcgactgc gatcacccag gtcatttacg aaatgtcacg caaaggcctg gggatgacga
      721 cggttataga agatggaaag ctggttgggg tcattagcga tggagacttg cgacgcctgc
      781 tcgagcgcca cggcaaggaa gtcttcgacc tcaccgccag ccagtgcatg acgcgggagc
      841 ccaaaactat cggccctgac gcatttgcca tcgccgccct cgacatcatg gaacaaaaga
      901 agattacttc gctgccggtg gtcgacgaca ctggttatct gctcggcgta ttgcacctgc
      961 atgacctttg gggaacgcag atggtgtgaa ctgcccggcc gcgggcattt gtcagccgga
     1021 ctatgtgaga gcctccggct ctaccgacgg cacggcccca acgtcagacg cttcaacaac
     1081 cggtatcgcg acgcctgcct cagctttctt cgcggtcacc gcggctacca cgacaaaaat
     1141 attggtgaac agcaccaccg tgaacgccat ggccgggaga aagaaaaata cctgtcctcg
     1201 ggccgccaat acctgcaacg ccagcacggc agtgatcagc ccccgcggca tcatcaggaa
     1261 tagcaactct gagtcggcgg gttgcacatc gcgtatcgac caccgcgtcg cccgcaccgc
     1321 aacatagcgc gccagcacca gggcgaccag gatgcccaga atcgggacca cgtacgcccg
     1381 gcttacgaat tgcgccatga ttccgagaag cacgaaaaag aaggagcgta ccaggaaggc
     1441 caactcggag tggaaggcca tcatccgcgc accttggcgt gtggtgtgcg gagtgcgcgg
     1501 catgttagcc aaagttaggc cgaagaccaa ctccgccagc agtccgctac cgccggcata
     1561 tcgacccgcc gcaaaaacac cgagcaccat gcccaggttc agcgcattgc taaactgctg
     1621 actcgcgaac tggggccata ctcgcgacca cgcaacaccg gcagcaacac ccaacaccac
     1681 atcaaccaag acgtggtgcc cgaatccggt aatcaggcct tggaagatgg gttggttgcc
     1741 atccagaccg atcagcgtac caaccgtgag caccgcgatg atctcgccca gcgatgcttc
     1801 cagggtcagc gttatcttga tggcttcggg cgcagcgatc tgctgaatgg cagggagcac
     1861 aaccgctgca ctggtcgatc ccagcaccgc gcccaacagc gcgcaatcca tccacgtcag
     1921 atgcagcaac accctggcca cggatgcaat cagtccaacg gtgcagctat agctgacgaa
     1981 tgccaacaat atcgcacccg gagagtagcg aaccgtctcc ttcaggcgca gttcgagtcc
     2041 accctcaaac aaaataagga tcagcgccag cattccgagg atacgaatca cgacgctgaa
     2101 gttgctgggg tcaacccaat gcaggatggg accaagtccc acaccgatga gcaacaatac
     2161 gatgatgtcc ggaacgcgtg tccagcgcga aagccggttc gcaatcacgg cgatcaccag
     2221 aagcgcggcg ggcaccccaa atatgcgagc cagatccatg atgccagatt caccgggtgt
     2281 agcagttcaa gtatgttgcc ggattttgcg acaaaaaacc agcgtttctt gcggtgtcgc
     2341 caccacggtt gaagcccctt gaaccgcgcc gaatcttcag acgccaacga aaaacccgac
     2401 ggcgatctca cgctggcgtg gaccgtcgag ttcaatcaga atcacgcgct gccatgttcc
     2461 taagaccagc gaacctttct ccgccagaag tccaacggac gtgccgatga ttgcagacaa
     2521 aatgtgatcg ggcacatgct ctggatcgtg cggatggcga tacttcagtt tgggaatgat
     2581 tcgctcaaaa gcatccagca tgtcggtatc ggtgccggga tcgaggtcgg ctgtggccag
     2641 cgccgctgtg gtatgcagca caacgaggtg gcacactccc agttggccgc catagtgatc
     2701 gctcagcaac gtctccacgc ggtcggtgat gtcaagaatc tcgcgtttgc ggttggtctt
     2761 gatcgtaagt cggtgcatgt gtccacattc taaagcgtcc tcaatcgccc aggctcgatc
     2821 tgaggctgtc gcctcgggtg gccagcgggt gtacgtctct gctagacttt cgcccgctgt
     2881 cacaaatccc gcctggactt gctgtagtaa ggagacgctt tgcctgccga gaatcccgat
     2941 cacgaacttg ccgccaacag ccgtagaggt tttctcaaag ctagcggcgt tgccctcgca
     3001 ggtactgcat tcctgcccgg actgctatct tcacagccca atcttgcata tccggcaaat
     3061 gacgctgtac cacatttcga attggacgaa acagacctgg cgacattgca gcaaaagctg
     3121 aaatccggtg agtatagttc caagaagttg gttgagttgt acaggtcacg catcgaaacc
     3181 atcgatcgcc acggccccgc cttaaatgca gtgatcgaga tcaattcgga tgcgaccgct
     3241 atcgcccaac aaatggatgc ggaacgaaag gcgaaagggc cacgcggccc cctgcacggt
     3301 atcccaatct tgatcaagga caacatcgcc actgcagaca agatgcaaac caccgccgga
     3361 tcgctcgcat tagtcgggtc cgttcccccg cacgactcag ccgtcgcagc caaactgcgc
     3421 gccgcgggcg ctgtcatcct cggtaaatca aacttgagcg agtgggcaaa ccttcgctcc
     3481 agtcattcaa ccagcgggtg gagtggacgc ggcggcttga ccaagaaccc gtacgcattg
     3541 gatcgcaatc cgtcgggatc gagttcgggt tccggcacgg cagtcgcatc gagtctctgt
     3601 gcggtcgccg tgggtagcga gaccgatggc tccatcgtct ccccgtccaa caactgcggg
     3661 atcgtgggca tcaagcctac agtggggctg atttcgcgga ctggcattat tcccatctcg
     3721 catacgcagg acactgccgg gccaatgacg cgcaccgtcg ccgatgccgc cgctctgctc
     3781 tccgtactgg ctggtgtgga ctcgcaggat ccggccaccg cagcggcagg cgaacacatc
     3841 cagttggact acacaaagtt cctcgacaag aatggattgc gtggtgcacg tatcggtgtc
     3901 gtccgcaagt acgctggctt taatactgat gtggatagcc tgtttgatga agccatcgaa
     3961 gccatgaagc atgcaggcgc tgaagttgtt gacccggtcg agattcccac catcggtaag
     4021 ttcgatgacg cggagttgct tgtgctttta tacgagttca aggacgactt gaatcgctac
     4081 ctcgcctggc tcggcgcgag tacgcaggtg cactcgatga aggacgtcat caagttcaac
     4141 gatgaccatc gcgatcagga gatgccatat ttcggacagg acctgatgct gcaatccgag
     4201 gccaaagggc cgctaacttc gccggagtac ctcaaggctc tcaatgactg ccgccgccaa
     4261 tcgcgcaccg agggcataga tgcggcaatg gacaagttca aactcgatgc tctgatcgcc
     4321 ccgactggtg gtccggcatg gacaactgac ttgctgaacg gtgatcacac cgtgggtggc
     4381 agctcgacgt tggccgcggt agctggctat ccgaacgtca acgtcccgat ggggttcacg
     4441 tttggaatgc ccgtcggggt ctcgttcttc ggtcgcgcct ggagtgagcc agtcctgatc
     4501 aagctcgcgt acgcctatga gcagttaacc aacgcacgca agctaccgaa tttttctccg
     4561 tccgctgatc tgtcgctgaa gagtcagaaa tagtgccgag caggaacaat caaggttcga
     4621 actgaggttc cgctcatgca agagagctaa aactagtcgc cggcatgcgc gtttccgttt
     4681 cggtagtgtt gttccggaag gttgcgaagt cgttctgccg cggattcagc cgtgatatcg
     4741 cgctgtggcg tggcaagcag ttcatatccc accatgaatt tcctcacggt tgccgagcgt
     4801 agcaatggtg ggaagaaatg cgcgtgcaag tgccactccg gatgctccac tccatccgtg
     4861 ggttgttggt gaaatcccat ggtgtacggg aatggagcct cgaaaagatt gtcgtagcgg
     4921 gtggtaatcc gcttcaatag gtcggccagc gccgtgcgag cgcggtcatc gagaccgcac
     4981 aaatcagcga cgtggctctt gctgcacagc agtatttcaa acggccaaac cgcccagaat
     5041 ggcaccagcg cgagaaagtg ctcgttctca cacacgatgc gttcccccgc cgcgcgctct
     5101 aactcgacat agtcgcacag caaacagctc ccgttgcggt ggcgatattc agtgagcgac
     5161 tcctgctcaa ggcgcggcac gttcggaatg gtttgatcgg cccagacctg cccgtgggga
     5221 tgcggattgc tgcatcccat catctcgcca cgattctcaa agatctgcac atggttgatc
     5281 tcccgcatcg cgcccagttc gcggtactgg tccgtccaga ggtccaccac tctgcgaatc
     5341 gaactcacgt ccatctgcgc cagcgtcata tcgtggcgcg gcgagaaaca gagcacccgg
     5401 cagactccgg tctcggtctg ggcgacgagc aacccctgcg ctccgtcact cacatcgtca
     5461 tttggcacat cgggcttcag cgcagcaaaa tcgttgtcga acacaaatgt tgaatcgtac
     5521 tgcgggtttc gaacgccgcc ggcgcgctca ttccccgggc acagatagca gtgcggatcg
     5581 aaacgaaact cgcgttcttc gggctgtctc tcggtctgcc cctgccacgg ccgctgggtg
     5641 cgatgcggcg aaaccagcac ccattcgcga gtcagcggat tgaaacgccg gtgcggaact
     5701 tctcggattt ttgagaggtc gatcatgcca ttaagtttgt gatttcgcct gcgccgtccg
     5761 ccgccgtaca cacgtatatc tcaggatgca gtcccatcgc gcgttcatac tcccgcgcaa
     5821 tcgtagcttt gaattcttcc acggcgccta cgtccaccag gttcaccgta caaccaccga
     5881 agccgccgcc ggtcatgcgt gctccataaa cgccgtggca tttcctcgcg aggtccacca
     5941 tcacgttgag ttccttgcaa ctcacttcgt aatcttcgcg taagctgaca tgcgactcgc
     6001 ccatgagggc tccgaatcgc gcgagtttgc cgtccctcag cgcctgcgcc gcttccagca
     6061 cccgcgcatt ctcgctaatc acatggcggc agcgccgata cgtgacgtcg gacaagccag
     6121 caccatactc tttcagttgt ggcaggctca catcgcgaag ggtctgaatt tccggcaatt
     6181 gccgcctcaa ataactgact ccagcctcgc aatccgcgcg acggcggttg tactccccgc
     6241 ctgcgagttc gtgcttcacc attgtgttgc acaccacgat gcggacgcgg tcgtcgatcc
     6301 tcaacaaatc gtaatcaagg ctgcggcaat ccagcagcaa tgcatggttg gctcgtccgc
     6361 agcatgagat gaactggtcc atgatgccgc acttcgtccc ggcgtactcg tgctcggcac
     6421 gctggcatgc tttcgccacc tggacgcggc tccactcaag ttccgagttc gtcatcaagg
     6481 ccagggcact cgcaacctcg atcgccgccg acgaactcag ccctgctcca atcggcactt
     6541 cacccttaat caccaggttt gcgccgcgaa tcgagttacc gctcgcctgc aataccgccg
     6601 cgactcctcg aacgtagtcg ctccagtggc ccgttggacc gcctgcgaga tggccgagat
     6661 cgaagttccg ggtctcgtca aagtcgattg aatagacact caatgtacgg tcgatacgcg
     6721 gcccagccgc gacgtaggta taaaactcaa gcgcggcagg catgacgaac ccatcgttgt
     6781 agtcggtatg ttccccaatg aggttcactc gtcccggtgc gcgaaagatg ccgattggct
     6841 ccccgtaaag tttcttgaaa gtctgagccg ccaggcgcat cgtaggtcgc gaattgtccg
     6901 ccacttccgc ttgttccata cgctgggaat ccggatcgcc tcccaccgag aatttctccg
     6961 ctcggaggcc agcctgccgg acaccaaagg tgtgccgcga aacatcatat tagacgcgca
     7021 tggatgtgta agggcgaatg tgccgggagg gacacgaaga gttacattgt gcgcaaatcg
     7081 ctcttcatca atagagccgc actcttggtc gccgaggcgt cacttgttga cgggcgaagc
     7141 tgtggcgcct cgcgaatttg ccattgcttc cgtcttggcc aaatcggcct tcacgcgttc
     7201 gttcagcggt ccgtcaggca ccagcttcaa ataggcgcga taatgatcgg ccgcagcagc
     7261 gggtgcgcct ttggcagtgt agattgacgc cagcaacagc tcgacgcgcg gctcggaatg
     7321 aagcttgtca aggctctcgg cccgcagcgc gttggtttca gcctgtggca ggtggttgag
     7381 attgaattca gccaccgcat tgtagtaata gcctagagga aaatttatgg gatcaagcgc
     7441 gagcagccgc tcggtgagtt tatgcgtctg ttcccagtta gaggccactg cggataaaaa
     7501 cgccatccga agatagggca aagcaaaatt cggatcggcg ttgatggctt cctgaaaatc
     7561 tgacgttgct ccttccatgt tctttcggcg ctgctccaga tcgccgagcc ttagccacgc
     7621 ttctgcgaac ttcggatatt cttcaatcgc tttgcgcagc aacttttcag cgtcagccag
     7681 acttcctttc tccagtgctt cgcttccctt ctcgtattcc tttcgagctt tcgcgggcac
     7741 ctgcatactt gctacgctga ccatcccctc aactttctta ccccggctat gcagcacgat
     7801 gactccaacg ttggtgttgt tcatcgaagc atccagacgt atggacgacg acgaatatcc
     7861 agagaggtct gcccgcagtt cgcaatccgc ggtgggggat gaaaaattat tctgccccgt
     7921 cgtcatgggg tcatttcttg aggtctggct gtcggagcca aacgaacggg tgtcctcgga
     7981 ggcgtccggc atcatactgg gggctcgatc tccaaccaca aaggagaagc cgccgtcacc
     8041 ggacgtgaat gtctcacgcc gactcgaact gttgcagatc cgcaggatcg cgaccggcga
     8101 aggcggtgcc ataccggtgt ccatcactac cttgccggag taatagaagg tctgaggcgg
     8161 aacagttggc gtaggagtga cgcgcgtcgg cgccttgggt tgcaccgtct tgctcccggc
     8221 cgcgcctccc tgctgggcgc ttgcactgcc cagtaagaac acaactatga cgagaactgc
     8281 ctcaatcagt tggaagcctt ttcgcatgat gcaccccaat ttcccccggt
//
LOCUS       DASRKK010000420         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_521692, whole genome shotgun sequence.
ACCESSION   DASRKK010000420 DASRKK010000000
VERSION     DASRKK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521692"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1869
                     /locus_tag="VFC15_09790"
     CDS             502..1869
                     /locus_tag="VFC15_09790"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HZM10488.1"
                     /translation="MSKHIRTHESNGTIDLAAVQIQPLTTSKDKSRARRLLGKHHYLG
                     DVRAVGEQLWYAITAGGIWLGVLVFCAASRRLRTRDQWIGWSEEQRRRRLPLIANNCR
                     FLLLPDKTFPNLASRSLSLASHRLSADWQIRYGHPVVVVETFVDPEQFCGTVYTASGW
                     QELGQTDGFGRVRRDFYVEHNKPKRLFARELCKNARRSLQADKLKPALALVEAKTRPR
                     CTQTAAEIRSLIHSLKRLPDYRRRIGIYPLWTLVAIALLAHLCGAPRGQKDLTKFAKG
                     MSQAQRRALGVRQQSDGYPAPSQPTFCRMMKHIDDAALERVILQTQEQIRGPAPPHEL
                     IVLDGKQPKHGGGQSVLTAVSVPSQHYLGSAMVDTKTNEIPVARELFKQLNLDGRKVS
                     LDALHTQDHTARDLVLEHGADYLLTVKDNQPTVRANIEKLVPALPAHFSPSSGDSQSG
                     GPHRV"
     gene            1895..2329
                     /locus_tag="VFC15_09795"
     CDS             1895..2329
                     /locus_tag="VFC15_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10489.1"
                     /translation="MRSRVATPEAVDFPCVEQVALLRRRLRQHKPETVALVTSLPPNQ
                     LNAAQWLADNRSAWGIENGLHLRLDVSHLDDQCRVRLPKSMRVMGMLRRFSNSLFMHW
                     RSRQTKPKYKTTTDFFTAMNAEHHRYAIRCLHACQPSFQTAS"
BASE COUNT          540 a          836 c          716 g          518 t
ORIGIN      
        1 cagcctctcc gcggaatatg ccgaccgtgt cgtcacgctc gaagccgccg gggagatcca
       61 atacgacacc tggggtggcc gcgaccatta tcgtccggtt cctcctgtca aagtgatctt
      121 caccggcacc ctctggcgtg cggaaaagca gcggcctgtc actcatccga cagattgacc
      181 gggcagtctt ccgcttttgc attctgcatt ctacatactg ccttccctgt gaggtggctt
      241 tgttggcgct ttgttggcgc gttagggcaa agggggaacg acctccggac aacgatgcac
      301 gcattgcaat gcaccccggc aggggtgcca gacgccgctc agacacagct caacttgttt
      361 ccttagcggc tctgaagtcc gtattctgtt cgggttgatc gagaatcacg gaaaattcct
      421 ctggcgaggt aagtagcgga aaaggatgga gttagccctt ggggctggac atgtcgcccg
      481 aagtgtttca cactttgggc catgtccaag catatccgca cccatgagag caacggcacg
      541 atcgacttgg cggccgtcca aatccaacca ttgaccacga gcaaagacaa gtcccgcgcc
      601 cgccgtttgc tgggcaaaca tcattacctg ggcgatgtcc gtgcggtcgg cgagcagctc
      661 tggtatgcga tcaccgctgg gggcatttgg ctgggcgtgc tggttttttg tgccgcttcc
      721 cgccgcttgc gcacccgcga tcaatggatc ggctggtccg aagaacaacg ccgccgccgc
      781 ctcccgctca ttgccaataa ctgccgtttc ttgctcctgc ccgacaaaac cttccccaac
      841 ttggcctctc gttccttgag cttggcctcg caccgtctgt cggctgactg gcaaattcgt
      901 tatggccatc cggtcgtagt ggtcgaaacc tttgttgatc ccgagcagtt ctgcggcacg
      961 gtctataccg ccagcggctg gcaggagttg gggcaaaccg acggctttgg ccgcgtgcgc
     1021 cgcgatttct acgtcgagca caacaagccc aagcgtctct tcgctcgcga actctgcaaa
     1081 aacgcccgcc gcagcctgca agccgacaaa ctcaagccgg cgctggccct ggtcgaagcc
     1141 aaaacccgtc cccgttgcac ccaaactgcc gccgaaattc gttccctcat ccactccttg
     1201 aagcgcctgc ccgattaccg ccgccgcatt ggcatctatc cgctttggac tctggtggcc
     1261 atcgccctgt tggcccacct gtgcggtgcc cctcgggggc aaaaggattt aaccaagttc
     1321 gccaaaggca tgagccaggc ccagcgccgc gccttgggcg tgcgccagca atccgacggc
     1381 tacccggctc ccagccagcc taccttttgc cgcatgatga aacacattga tgatgcggcc
     1441 ctcgaaagag tcatcctcca aacccaggaa caaatccgcg gcccggctcc gccccacgag
     1501 ctgattgtct tagacggcaa acagcccaag cacgggggcg gccaaagcgt gctcaccgcc
     1561 gtgagcgtgc ccagtcagca ctatttgggc agcgccatgg tggacaccaa gaccaacgaa
     1621 atccccgtgg cgcgcgaact ctttaagcag ctcaacctgg acggacgcaa agttagtttg
     1681 gacgcccttc atacccagga ccacaccgcc cgcgatctgg tgctcgaaca cggcgccgac
     1741 tatctgctga cggtcaaaga caaccaaccg accgtgcgag ccaacattga aaaattagtc
     1801 cccgcgttgc cagcgcattt ttccccctcg agcggcgact cccaatctgg cgggccgcat
     1861 cgagtttaac aaaggcgagc tggaagtccg ggtcttgcgt tcgcgcgtgg ccacccctga
     1921 agcagtcgac tttccctgtg tcgaacaagt ggccttgttg cggcggcgct tgcgtcagca
     1981 caagccggag accgtcgcgc tggtgaccag cttgccccca aaccaactca acgccgccca
     2041 gtggttggcc gacaaccgct ccgcttgggg cattgagaac ggtctgcatc tacggctgga
     2101 cgtcagtcat ctggatgacc agtgccgcgt gcgcctcccc aagtccatgc gggtgatggg
     2161 gatgttgcgc cggttcagca acagtctctt catgcactgg cgcagtcgcc agactaagcc
     2221 aaagtacaaa accaccacgg atttctttac cgccatgaac gccgaacatc atcgctatgc
     2281 tatccgttgc ctccacgcct gccagccaag cttccaaacc gcttcatgaa ttagccctgc
     2341 tgggcgagga atagtgaaga attcaactcg acaagacggt tcccgaaatg ggaacatctc
     2401 gcttgcctcg cgacaattcc tgtgagaatc tatgcgcgta atatcgagaa agtggttgaa
     2461 tgagctggcg agaacacatc cggatgcgaa gcctgggctg gacgcctggt tccatgaggc
     2521 cgagggcgcg gattgggcca acccggcgca agtcaaagag caatacgcca cagcgagtat
     2581 tttgaagaat ggccgggtcg tgttcaacat
//
LOCUS       DASRKK010000421         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_326137, whole genome shotgun sequence.
ACCESSION   DASRKK010000421 DASRKK010000000
VERSION     DASRKK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326137"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFC15_09800"
     CDS             complement(<1..1002)
                     /locus_tag="VFC15_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZM10490.1"
                     /translation="MNWLNAWFGFDRHRTSVGREVVAGLTTFTTMSYIVVVNPAILSM
                     AGIPAGPSFVATVLVAAFGCYLMAFYANRPFAIAPYMGENAFIAFTVCKLLGFRWQTA
                     LAAIFIAGAVFILMTILRLRQWVVEGVPTSLRYSFAVGIGLFLTFIGLNQTGLVTLGV
                     PGAPVQAGHLTSPPALVAMLGFVLIAILVIRKVPGAILLGIVITAIVAFIAKTAPPPH
                     GLVSIPPSIAPILWQLDLRGAFTWRAFPVVLTIFIMAFVDTMGTLIGLSARAGFLDDN
                     GQLPHIERPMLVDAITTCIAPVLGTTTAGAYLESATGIEAGGRTGFTVFVTGSCFLLT
                     "
     gene            complement(1135..>3138)
                     /locus_tag="VFC15_09805"
     CDS             complement(1135..>3138)
                     /locus_tag="VFC15_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM10491.1"
                     /translation="TTFDVSMLSKKVEKQEITVNASRLDMPLAANPAATSIVVPETLS
                     QMPRGVAPEEALEGIPSVKCDNQADGERVHVSIRGQGILSEHGIRGIEVLLDGIPLSD
                     PTGFVPDLFDVDWNGLQDVQVMRGPVAVLYGGGSAGGVIDLHTRLPQDTTNVSFWSTG
                     GSYGFYKARGEVSGTAKGMGYDLALSRAAGDGYRQHTSFWGDILSGRLYFKPKPSFRL
                     NVFGIGTGYFNQNAEGLNLTWLAENRRQANPDALKYNEYQKTTRFSGGVTGDWTASDN
                     QHLAFNFFSRPTNYTESVPSSVLHRSLTPLEGSVQYNFDHVGTTIRNYLGVGFDLGGQ
                     FIDATAYPNLGNAVEGPNLVANGNITQSHVGAYVMDRVSFGPKWTLFLSGRWDRITNR
                     YADNLKFNGLDLSGSRVFTKPTGRVGVSWTPRQEFTAYTSWGSGFLPPATEELLANPD
                     SIGGFNTHLVPATSQGPDVGVRGNLGFHLYYDVTFYYLQTKNDFERYRITDRPLETFY
                     ANGGNSNRYGLETEARWLPTRWLTISGAYTFSHMTYYSYLSHTYPGNLNGHYLPNIPM
                     NQLYADALLQLPMKFSISARTISFSRAYIDPTNATWIGSYTLLGARVAKGWQYKNFWG
                     SVFLTGRNLTNTQYIAFTEPDPDGNSYQPGPDREIFGGLEFHF"
BASE COUNT          720 a          873 c          925 g          620 t
ORIGIN      
        1 cgtcagcagg aaacaacttc cagtaacgaa gacggtaaac ccggtgcgcc cgcccgcctc
       61 tattccggtg gccgactcga ggtaagcgcc ggcggtcgtg gtgcccagga cgggagcgat
      121 gcaagtcgtg atcgcgtcta ccagcatggg acgctcgatg tgaggcagtt gtccattgtc
      181 gtcgaggaaa ccagcgcgtg ccgaaagccc aatcagggtg cccatggtgt ctacaaacgc
      241 catgatgaag atggtgagca ccactgggaa tgcacgccag gtgaaggcgc cgcgaagatc
      301 aagctgccac agaatcggcg cgatactcgg cggtatgctg accaggccgt gtggcggtgg
      361 ggcggttttg gcaatgaacg ccacaatcgc ggtgattacg atgcccaaca ggatcgctcc
      421 ggggaccttt cggatcacca gaatcgcaat caacacgaag cccaacatgg ctactagcgc
      481 gggaggcgag gtcagatgcc ctgcctgcac cggagcgccc ggaacaccga gggtgaccaa
      541 tccagtctgg ttcagtccga tgaaggtgag aaacagtccg attcccaccg cgaagctgta
      601 gcgcagcgag gtaggcacgc cttccaccac ccactgccgc agccgcagga tagtcatcag
      661 gataaacacc gcgccggcga tgaagatcgc cgccagcgcc gtctgccagc ggaagcccaa
      721 aagcttgcaa accgtgaagg ctatgaacgc gttttcgccc atgtaaggcg ctatggcgaa
      781 aggacgattg gcgtaaaacg ccatcaggta acaaccgaac gccgccacta gcactgtcgc
      841 cacaaacgat gggcctgccg gaattccagc catggaaaga atggcgggat tgactaccac
      901 aatgtaggac atcgtggtga acgtcgtcag cccggcgacc acctcgcgtc ccaccgaggt
      961 gcgatgacga tcaaaaccga accaggcgtt cagccagttc aaagggcgct ccggaataca
     1021 gaggcatggt cgaacggcgc ggcatgcttg tcaatagcag gccggcgata aaaatcggtg
     1081 gatccaaagt gtcggaccca ccggaggtgc gtacgagagg tcgcaatagg agatctaaaa
     1141 atggaactcc aagccgccga agatttcgcg gtccggtcca ggttgatagg agtttccatc
     1201 cggatcgggc tcggtgaagg cgatgtactg cgtgttagtc aggttccttc cggtaaggaa
     1261 cacactgccc cagaagttct tatactgcca gccttttgcg accctggcgc ccagcaaggt
     1321 gtaactgcca atccaagttg cgttggtcgg gtcaatgtag gcccttgaaa agctgatcgt
     1381 ccgcgcggaa atcgaaaact tcatgggaag ctgaagcaga gcatccgcat acagttggtt
     1441 cattgggatg ttcggcaggt agtggccgtt cagattgccg ggataggtat ggcttaggta
     1501 gctgtagtaa gtcatgtgcg agaacgtgta ggcaccggaa atggtaagcc aacgtgttgg
     1561 cagccatcgc gcctcggttt ccaggccata gcgattgctg ttgcctccat tggcgtagaa
     1621 ggtctctaga gggcgatccg taatccgata acgttcaaag tcgttcttgg tctggagata
     1681 atagaatgtg acgtcgtagt acagatggaa gcctaggttg ccgcggaccc cgacgtctgg
     1741 cccctgagaa gtggctggaa ccaggtgagt attgaaaccc ccgatggaat ccgggttggc
     1801 caaaagttcc tcggtcgctg gaggcagaaa tccggatccc caggatgtgt atgccgtgaa
     1861 ttcctggcga ggcgtccagc ttaccccgac gcgtcctgtc ggtttggtga atactcggct
     1921 gcccgacagg tcgaggccgt tgaatttcag gttgtcagcg tatctattgg tgatgcggtc
     1981 ccaacgtcca ctcaagaaca acgtccactt cggtccaaag ctgacgcgat ccataacata
     2041 ggcgccaacg tgactctggg tgatgttgcc attggcaaca aggttaggcc cctcgaccgc
     2101 attgccgagg tttgggtaag cagtggcatc gatgaactgc ccacccagat cgaatcccac
     2161 cccgagatag ttcctgatcg tggtgcctac gtgatcaaaa ttgtattgca ccgagccttc
     2221 caggggtgtg agcgaccgat gaagaacgga cgagggcact gactcggtgt agtttgtcgg
     2281 tctggagaag aaattgaagg cgagatgttg gttatcggaa gcggtccaat ctccagtaac
     2341 gcccccgcta aagcgcgtcg tcttctgata ctcgttgtac ttgagggcgt ccgggtttgc
     2401 ctgtcggcga ttttctgcga gccaagtgag gttgagacct tccgcgttct ggttgaagta
     2461 tccggtgcca atgccaaaca catttaagcg aaacgacggc ttcggtttga agtacaaacg
     2521 cccggacaaa atatctcccc agaaggaggt atgctgccga tagccatccc cagcggcacg
     2581 cgacagggcc aggtcatagc ccatgccctt ggccgttccg gacacttcac cgcgcgcctt
     2641 gtaaaaaccg taggagcccc cggtcgacca gaagctgacg ttcgtcgtat cctgcggcag
     2701 gcgcgtatgc aagtcgatta cgcctccggc cgaaccgccg ccatacaaaa cagctaccgg
     2761 ccctctcata acttgcacgt cctgaagtcc attccagtcg acgtcaaaaa gatcgggaac
     2821 gaagcccgtc ggatcgctca gcggtattcc gtcgagcaac acctcgatac ccctgatacc
     2881 gtgctcgctg agaatgccct gtccacggat cgagacgtgt acacgttctc cgtcggcctg
     2941 gttgtcacat ttcacgctgg gtatgccctc caacgcctct tccggtgcga cgccccgagg
     3001 catttgcgaa agcgtctcgg gcaccacgat ggaagttgcg gcgggattcg ccgccagcgg
     3061 catgtcgagc cgggatgcat tgaccgtgat ttcctgcttc tcaactttct tcgaaagcat
     3121 ggaaacatcg aatgtggt
//
LOCUS       DASRKK010000422        14821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1205908, whole genome shotgun sequence.
ACCESSION   DASRKK010000422 DASRKK010000000
VERSION     DASRKK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205908"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1695
                     /locus_tag="VFC15_09810"
     CDS             163..1695
                     /locus_tag="VFC15_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM10492.1"
                     /translation="MATVEQAVTKTAAGKATVYKNFINGEWVESHTGRTFENLNPADT
                     RDVVGVFQRSDADDVNRAIDAASEAYKKWRLVPAPRRAEMLYKASEILIGRKEEYAQL
                     MTREMGKVIKETRGDVQEAIDCGYYTAGEGRRLFGPTVPSELPNKFAMAIRQPLGVCA
                     MITPWNFPMAIPSWKLFPALVAGNTAVIKPAQDTPLSTFNFVQALTDGGIPAGVVNVV
                     SGYGTEVGTPMTQHAEVRAVSLTGSTAVGRVVGQEAAKTFKHCSLELGGKNPMIVLDD
                     ANLELALEGALWGAFGTTGQRCTATSRIIVQKGVYKKFVDELVARAQKLKVGNGLDES
                     VDMGPAVNKSQMETDLKYIEIGKGEGAKLMCGGQRLEGGAWEHGWFVAPTVFADVNPK
                     MRIAQEEIFGPVVSIIPCDGLENAIEIANDIEYGLSSSIYTKDVNKAFTAMRDLYAGI
                     TYINAPTIGAEVHLPFGGTKATGNGHREGGIGAIDFYTEWKSIYIDYSDKLQKAQIDR
                     PE"
     gene            1872..3086
                     /locus_tag="VFC15_09815"
     CDS             1872..3086
                     /locus_tag="VFC15_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM10493.1"
                     /translation="MPTKTATEVLAASRMANVRYAIRDLAVVADEVAHEGHKILYLNI
                     GDPCKFDFPTPPHMIEAVHKAMRDGHNSYGESLGIKPAVDAIRRQAEANGFKDIQSIF
                     VGYGSGEVIDSCLTALLNPGDNVLTPSPEYPLYGAVLAKLDAVSNAYDLDESNGWEPD
                     LADLERKINPRTRAILLINPSNPTGAVMSRRTLERVLELARRHNLLILADEIYDKLIF
                     DPQEKHISIATLAQDVPIITFNGLSKAYLVPGWRIGWAIATGPRESLHHYLEAVHRLL
                     RARLSAPHPFQHAISHALEGPQDHIPVMLEKLRRRAQITVDWAKRTPRVSLVAPKGAF
                     YAHPSLDIPEDDLTFVTDLLKQKHVLVVHGSGFGQKPGTRHMRIVFLPQDEVLNAAYE
                     KITDFMQERYKD"
     gene            3197..3925
                     /locus_tag="VFC15_09820"
     CDS             3197..3925
                     /locus_tag="VFC15_09820"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZM10494.1"
                     /translation="MVDIHCHILPAIDDGPDSWEMTAKMCRLAALDGITHIVATPHCD
                     AQFDYDREHFTDMLATLSEVANGRLTFSIGCDFHISPHNVADAMADPQRFAIGDSQYL
                     LVEFDHHGIPSNASELLMALISRGMVPIITHPERNAFLIKHPETVLRFVESGCLVQVT
                     ANAFTGFWGPKSRKAAEWLLHRDAIHVVASDAHDLNLRPPVLSEAHDRVAALAGVEAA
                     DALVTHNPAAIVAGQHVPFSPARR"
     gene            complement(3968..4885)
                     /gene="nikB"
                     /locus_tag="VFC15_09825"
     CDS             complement(3968..4885)
                     /gene="nikB"
                     /locus_tag="VFC15_09825"
                     /inference="COORDINATES: protein motif:HMM:NF045470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HZM10495.1"
                     /translation="MLRYIFSRLLYMLPVIWLVVSVVFLLIHIVPGDPIQQMLGEGAP
                     AADIAAARHAYGLDVPLGTQYVNYWRGLLHGDMGRSFRFNKNVGQIIAQAYPWTLQLT
                     LAALTVALCLAIPAGVRSALHRNRWDDRALSFVSLLGLSFPNFALGPILILLFAIELG
                     WLPVSGSGSWQNLVLPAITMGGALAAILTRMVRTSMLEELGQDYIRTARAKGLTERAV
                     VYRHALRNAMIPVLTVVGLQFGAMLAGAIVTETIFSWPGIGRLTVQAISSRDYYLVQG
                     CILVIGLTYVLVNFLTDLMYSALNPRIRQ"
     gene            complement(5293..6663)
                     /locus_tag="VFC15_09830"
     CDS             complement(5293..6663)
                     /locus_tag="VFC15_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZM10496.1"
                     /translation="MVTFVVLRIVAVVLLVAANAFFVAAEFALVSVRETRLQQLIAAH
                     RIGARTVQKLHQKLDEFIAAVQFGVTVSSLALGWIGEAAMARVLEPLFVRLPYSHIYA
                     HAFSIGFAFILITYMHVILGEIVPKSLALQRTESVALAVAGPMDVFMTLAAPLLVVMT
                     ASSRVVLKAFGARQVREGGVHSPEELKLIVSASRRIGILEAPQEQMIHRALDLENVFV
                     REIMVPRMDIFSLPGDMPLDEAFQKVADETHSRVPVYDPERGPEHIIGVLYAKDLLRW
                     LRYRLGQLMTGQPQSRSSDLKVRHVMREVLVVPETKPLPDLLMEFKQRKRHLAVVVDE
                     FGSTAGVVTVEDILEQLVGEIEDEFDISDPVFAPGDSSIVLNGAVNIRDLESQYKINL
                     PRDEGFETLAGFVLARLQRIPALGDSFEYEGRRYSVVSMDGLRVDTVKIEAPKPQTPA
                     AKSAVQ"
     gene            6904..8115
                     /locus_tag="VFC15_09835"
     CDS             6904..8115
                     /locus_tag="VFC15_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM10497.1"
                     /translation="MANGKNHSKKKRLVIISIVAVAVVALVIVAVAATRGGTKIDPTK
                     LAKVEKGDLAKSVVATGKVTPITKVEIKSKASGIVKKLYVDAGDTVKKGQVLADLDRD
                     EIEAQVNSSRAQLLSSEANQKGAEADMTRAEVDAQGVDIPTLQRAYERAQEMAKGGIV
                     STSALDDAQRMYILAVNKRDVARAQLVVNKAKVAQAKADVQKSDATLKQLEEQLSYTT
                     IVAPIDGIILSRDVEMGDAVSSILVMGSAATLVMTIGDTSEVYVKGKVDESDIGKVYL
                     GQPARIRVETFKDKTFTGHVTKISPMGAEKDNVTTFEVRVSINNPGGELKAEMTANAE
                     IILEEHKNVLMIPEGAILYDKDKKASTEVPDPKGKEGKRKVALNIGISNGAKTEVVSG
                     LKEGDQVVLQQ"
     gene            8198..9109
                     /locus_tag="VFC15_09840"
     CDS             8198..9109
                     /locus_tag="VFC15_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM10498.1"
                     /translation="MKKHFIATLPVILLVPVLLLARVEDQFDRTLPVSGSVNLDLTTG
                     SGDVIIKTGGSNQVIVHGRVRSNNEWFGDNADRLVHSVASNPPIEQNGGTIRIGYNLP
                     EDAKHHISISYEVTVPADTTVNAGSGSGEISVQGVRSPVKLHTGSGDIRVQDLGPQSH
                     VDTGSGSIHAESVAAPFSAGTGSGEIQADLTGSGDVDVHTGSGGIRIRGIKGGLSART
                     GSGSVGADGDVKGPWQIHSGSGNIKMALSSGGGFNLDVHTSSGSIHSDLPVTMQGTLG
                     RHELKGAVRGGGPEVEVSTGSGDVDIR"
     gene            complement(9136..9780)
                     /locus_tag="VFC15_09845"
     CDS             complement(9136..9780)
                     /locus_tag="VFC15_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZM10499.1"
                     /translation="MHVAELWRCPVKSMRGEQLQQAEVLQTGVRGDREIVVVSTSRRR
                     MITARTHHRLLGLQGGISPETHAATINGYSWDSPEARALVEEAVGEKAELVHVPGTER
                     FDVLPLLVATDGALDALGVDRRRFRFNILIGGVEGLAERGWEDKQLRIGEVEIYAAQL
                     RARCVMTTYDPDTQKQDRNVLFRIVKEFDGTMALDCSVVKPGTIRVGDPVTIVE"
     gene            9899..10705
                     /locus_tag="VFC15_09850"
     CDS             9899..10705
                     /locus_tag="VFC15_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZM10500.1"
                     /translation="MAEPRVVTFTSDFGLDDPFVGIMHGVVLSIHPETRMVDICHAIP
                     SFNVLDAAWTIAQAYRFFPPRSVHVVVVDPGVGSARRPILAETDDYIFVAPDNGVLSL
                     VEAREPKLTVRHITADRYFLQPVSQTFHGRDIFSPVAAWLSKGVAPAEFGPEVTDYVR
                     LPLPAVEHIGENSLRGAVLKVDKFGNLITNLSEQEAPALFAATAPSVQLLIAGQTITR
                     VCRSYAEGGDDEFFAILGSSGYLEIAARQASAAERLSAGVGAPVGVILQE"
     gene            complement(10712..10954)
                     /locus_tag="VFC15_09855"
     CDS             complement(10712..10954)
                     /locus_tag="VFC15_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10501.1"
                     /translation="MKHIGYILRFLWNATRGHHLTPWRSSYLRWRIETYTGLKMQSIG
                     FLEFWGFSWRERRELIRFLTWTGEMERYARVKPKNP"
     gene            11068..11433
                     /locus_tag="VFC15_09860"
     CDS             11068..11433
                     /locus_tag="VFC15_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10502.1"
                     /translation="MTNDTSRQAAEDRYYAALDHYAEGRLTEAIADYEAAIATDPTFL
                     DAMHGLARAYQDAEQFDQAITVANRITELDPDDVLAHTSLSILYQRKGMVPEAEAEAN
                     KARILGWKQQLKQGKTSQP"
     gene            11430..12323
                     /locus_tag="VFC15_09865"
     CDS             11430..12323
                     /locus_tag="VFC15_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10503.1"
                     /translation="MTSRAHSARKTADKLRHVSSPAQTVRAIYRKLSRAWGPQHWWPA
                     ETPFEVITGAILTQNTSWTNVERALSKLREAGMLSVEGLSQLPLDGLEQLVRSSGYFR
                     QKALRLKSFIAFLDENHGGSLDSLLATPTEQLREELLAQKGIGPETADSILLYAGHHP
                     IFVVDAYTRRVLERHDAVAADAKYDEIRALVEHALQSEKPSPAVAPGSLDQQRPAVHP
                     PSAMSTAPRALSAQVFNEMHGLFVQLGKHYCHKQQPDCDVCPLGAMLSHPVIPTLTRN
                     SQQRSSGNKRRASKKPAKSRL"
     gene            12403..14676
                     /locus_tag="VFC15_09870"
     CDS             12403..14676
                     /locus_tag="VFC15_09870"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM10504.1"
                     /translation="MSPVTPPNRPDHKRSRTPAIVGLSIAVFLLFAIIFSQATFNLTF
                     LQPDTSEETLIFAALSALIFLLFVALTFVLLRILLRLYAERKTGVMGSKLRSRMVMGA
                     LLLSLGPVIFLFLFSYGLINRSIEKWFSRPVEDVQYRTGVVAALLTDYAADNARVEAE
                     RIASSPAAKKSFETDNFTLVMEEFRRSEVSLQGGFAFAVANDRAEASFHAPQPWPLLH
                     DRLPMKESAENKPKSFDILGKTYVLGRAVVGPGAQILVAMPLPANYSLALRDIEQAQQ
                     QYQELRKQRRRLRRTYLGYILMLTVGTLFASTWLALYLSKMVTRPLVALAEATQEISR
                     GHLDYRVDVQVGSEIGQLVNSFNQMAADLEASRASIEASRADLADVNVQIEQRNRHIE
                     TILESIPTGVLSLDASRHVVHMNGAVERLLHLDPSPAQAARTLEELLPDDVTEDLEHL
                     MRKADRMGTTTSQMEIVTSRVNLNVAVTVASLDSPARVQEPLAQRMGYVVVLEDMTDL
                     LRAQKQTAWSEVARRVAHEIKNPLTPIALSAERIRRHLERGAPPDAASLKIIRNCVDT
                     IGSSVQTVRTLVDEFATLARFPASRPMPSDINSIVESALLMFDGRLSGIRVQKILASG
                     LPPVHADPDAIKRAIANLVDNAAEAMQGSMVKEIVISTALAEERDSVEIVLSDTGHGV
                     TPALKEKLFLPYFSTKKRGTGLGLAIVSRIVEDHHGSIRVEENTPIGTRFIVELPVAS
                     AMAVEPSEQHAHHSDRG"
BASE COUNT         3116 a         4476 c         4344 g         2885 t
ORIGIN      
        1 ggtagggagg ggggtacttc acgtcgaaac aatggcggtg ccccaggttc gcgtccttcc
       61 tttggacgcc aacctgggtg ataaaggcgc tgcccttatt tgccacccgc tcgctgcgcc
      121 cgataccata gagggtttgc acttaaagat ttggagataa ttatggcaac cgtggaacag
      181 gcagtgacca aaacggcggc gggcaaagct accgtgtaca agaacttcat caacggcgag
      241 tgggtggaga gccacaccgg acgcaccttt gagaacctga accccgccga cacgcgcgat
      301 gtggtcggcg tgtttcagcg ctcggacgcc gacgacgtga accgcgccat cgatgccgcc
      361 agcgaagcct ataagaagtg gcggctggtg ccggctccgc ggcgggccga gatgctctac
      421 aaagcctccg agatcctgat cggccgcaag gaagaatatg cgcaactgat gacccgcgag
      481 atgggcaagg tcattaagga gacgcgcggc gacgtgcagg aagccatcga ctgcggctac
      541 tacaccgccg gagaaggccg ccgcctgttc ggcccgaccg tgccctcgga actgcccaac
      601 aagtttgcca tggccatccg gcagccgctg ggcgtctgcg ccatgatcac gccgtggaat
      661 ttccccatgg caattccctc atggaagttg ttccccgcgc tggtcgcggg caacaccgct
      721 gtaatcaagc cggcacagga cacgccgctc tccaccttca attttgtgca ggcgctgacg
      781 gatggcggca ttcccgcggg cgtggtgaac gtcgtcagtg gatatgggac tgaagtcgga
      841 acgccgatga cgcagcacgc cgaggtgcgc gcggtgtcac tgacgggttc gacggccgtg
      901 ggacgcgtgg tggggcagga ggcggccaag acgttcaagc actgctcgct ggagttgggc
      961 gggaagaatc cgatgatcgt gcttgacgac gcgaacctcg agctggctct ggagggcgcg
     1021 ttatgggggg cgtttgggac gaccgggcag cggtgcacgg cgacgagccg catcatcgtg
     1081 cagaagggcg tgtacaagaa atttgtggat gagctggtgg cgcgcgcgca gaagctgaag
     1141 gtcggcaacg ggctggatga gtcggtcgac atgggtccgg cggtcaacaa gtcgcagatg
     1201 gaaaccgacc tgaagtacat cgagataggc aagggcgaag gcgcgaagct gatgtgcggc
     1261 gggcagcggc tggaaggcgg cgcgtgggag catggctggt tcgtggcgcc gacggtgttt
     1321 gcggacgtca atccgaagat gcggattgcg caggaagaaa tctttgggcc ggtggtttcg
     1381 atcattccgt gcgatggcct ggagaacgcc atcgagatcg ccaacgacat cgagtatggc
     1441 ctgtcgtcgt ccatctacac caaagatgtg aacaaggctt tcaccgcgat gcgcgatctg
     1501 tatgcgggga ttacgtacat taacgctcct acgattggcg cggaagtgca tctgcccttc
     1561 ggcggcacca aggccacggg caacggtcac cgcgaaggcg ggatcggtgc catcgatttc
     1621 tataccgagt ggaagtcgat ttatatcgat tattccgaca agctgcagaa ggcacagatc
     1681 gatcggccgg agtagaggaa gcacttagca attagcgatt agctaagtac cgcagttatc
     1741 ggccgcggat ttttacgaat caacacggat ttgtttttag catgcttaag acctaatctg
     1801 atccgtgaac ctccgcgtga atccgcggct gatttaacat cgcaatccgg caaactgtaa
     1861 gggtcttcat catgcctacg aaaactgcaa ctgaagttct cgctgccagc cgcatggcca
     1921 atgtgcgcta tgccattcgc gatctcgcgg tggtggccga cgaggtcgcg cacgaaggcc
     1981 acaagatcct ctacctcaat attggcgacc cttgtaagtt cgactttccc acgccgccgc
     2041 acatgatcga ggcggtgcat aaggcgatgc gcgatggcca caacagctat ggcgaatcgc
     2101 tgggcattaa accggcggtt gacgccattc gccgccaggc cgaggccaat ggcttcaaag
     2161 acattcagag catcttcgtc ggctacggca gcggagaggt catcgacagt tgcctgacgg
     2221 cgctgcttaa tcccggcgac aatgtgctga cgccgagccc cgagtatccg ctctacggag
     2281 cagtgctggc caagctcgac gccgtttcca atgcctacga cctcgatgag tcgaatggct
     2341 gggagcccga ccttgccgat ctcgagcgca aaatcaatcc ccgtacccgg gcgatcctgc
     2401 tgatcaatcc cagcaatcct acgggcgcgg tgatgtcgcg gcgaaccctg gagcgggtac
     2461 tggagttggc gcgccgacac aatctgctca tcctcgcgga tgagatttat gacaagctga
     2521 tcttcgatcc gcaggagaag cacatctcga tcgccacgct ggcgcaggac gtgcccatca
     2581 tcacgtttaa cggcctgtcg aaagcgtacc tggttccggg atggcgcatt ggctgggcca
     2641 ttgctaccgg gccgcgcgag tcgctgcatc actatctgga agccgtgcac cgcctgctgc
     2701 gtgcaaggct ttcggcgccg catccgtttc aacacgccat cagccacgcg ctcgaaggtc
     2761 cgcaggacca cattccggtg atgctcgaga aactgcgtcg ccgcgctcag atcaccgtcg
     2821 attgggcgaa gcgtacgccg cgcgtcagcc tggtcgcgcc gaagggagcg ttctacgcgc
     2881 atcccagcct cgacattccc gaggacgacc tgacttttgt caccgacctg ctgaagcaga
     2941 agcacgtgct ggtggttcac ggctccggct tcggtcagaa accgggcaca agacacatgc
     3001 ggatcgtatt cctgccgcag gacgaggtac tgaatgcggc gtacgagaag atcacagatt
     3061 tcatgcagga gcggtacaaa gattagcaac tagcaattag cagctagctg ttcagcgggt
     3121 gactggtaac tggtgactgg tagtcggctt aacttctagc ttcggcccgc agccagctac
     3181 gcgatgacaa agacagatgg tcgatattca ctgccacata cttcccgcca tcgatgacgg
     3241 tcctgactcg tgggagatga ctgccaagat gtgtcgcctg gcagccttgg acggcattac
     3301 gcacatcgtg gccacgccgc attgcgacgc ccaattcgac tacgaccgcg aacacttcac
     3361 cgacatgctg gcgacgttga gcgaggtcgc taacggccgt ctcactttca gcatcggttg
     3421 tgactttcac atttccccac acaacgtcgc agacgccatg gctgatccgc aacgcttcgc
     3481 catcggcgat tcccaatact tgctggtgga gttcgatcac cacggcattc cttccaacgc
     3541 gagcgagtta ctgatggcgc tgatcagtcg cggcatggtt ccgatcatca cccatcccga
     3601 gcgcaatgcc ttcctgatca agcaccccga aaccgtgttg cgatttgtcg aatcgggatg
     3661 cctcgtacag gtcacggcga atgcgttcac cggattctgg ggcccgaagt cgaggaaggc
     3721 agcggagtgg ttgcttcacc gcgatgcaat ccatgtggtc gccagcgatg ctcacgattt
     3781 gaatctgcgc ccaccggtgt tatccgaggc gcacgaccgg gtcgctgcgc tcgccggagt
     3841 cgaggcggcc gacgcgctgg tcacgcacaa tcccgctgcg atcgtcgcgg gacagcatgt
     3901 tccattctcc ccagcacgtc gttgatccct gagtttgccg ggcgccgacg ctgggtaggg
     3961 agatcggcta ttgtctgatc cgcggattca gcgctgaata catcaggtcg gtaaggaagt
     4021 tcaccagcac gtaggtcagg ccgatcacca ggatgcagcc ctgcaccagg taataatcgc
     4081 ggctggagat cgcctgcacc gtaagtcgcc cgatgcccgg ccaggaaaaa atcgtctcag
     4141 tcacgatggc tccggcgagc atggcgccaa actgcaagcc caccacggtc agcacaggaa
     4201 tcattgcatt gcgcaatgcg tggcgataga cgaccgctcg ctccgtcagt cccttggccc
     4261 gggccgttcg gatgtagtcc tgcccgagtt cctcgagcat cgaggtgcgt accatgcgcg
     4321 tcaggatcgc cgccaatgct cctcccatgg taatcgccgg aagcacaagg ttctgccatg
     4381 agcctgatcc cgacaccggc agccagccca gttcaattgc gaataacaag atgaggatcg
     4441 gtcccagcgc gaagtttggg aacgacaatc ccagcagcga gacgaagctg agcgcgcggt
     4501 cgtcccatcg attgcgatgc aaggccgaac gtacgcccgc tggaatcgcc aggcatagcg
     4561 ccacggtcag cgccgccagt gtcagttgca acgtccatgg atacgcctgc gcaatgatct
     4621 gtccaacatt tttgttgaat cggaacgaac gtcccatgtc gccgtgcagc aggccgcgcc
     4681 agtagttcac atattgagta cctaacggca catccagccc atacgcgtgg cgcgcggcgg
     4741 cgatgtcggc tgcgggtgcg ccttcgccca gcatttgctg aatgggatcg ccgggcacaa
     4801 tgtgaatcag caggaagacc accgacacta ccagccagat taccggcagc atgtacagca
     4861 ggcgactgaa gatgtagcgg agcatcggaa tccgtatcct aaatggtggg cgtgatggaa
     4921 ttcgaattcc ctcaggggct aaagagtctg tgtgagaacc tcgatcgtcc ccgggggact
     4981 caaatctttt tcccacctta cccagcactc cgccctccca ccccaacccg cgcaaaagcg
     5041 gcgcggctcg gggaccccgg ttcggctgcg tgctgggcta tcatgtcacc gcccccgtgg
     5101 ggctggactt ctggcagctt tcaccaccgt cgcaagccga gcattagttc tcacacagac
     5161 tctaaagccc aatgttcaac gagccaggag cggcacgact gaagtcgtgg cctgatacaa
     5221 agccgtcgcg aatcgagttt ttcagcagcc tctaaagcag taccctttca aatcgtatct
     5281 atgaagccct tcctactgca ccgccgactt tgccgccggc gtttgcggct tcggcgcttc
     5341 gatcttcacc gtatccacgc gcaggccgtc catcgaaacc acggagtagc ggcgtccttc
     5401 atattcgaaa ctgtcgccca gcgcgggaat ccgctgcagt ctcgcgagca cgaagccggc
     5461 cagggtctcg aagccttcat cgcgcggaag gttgattttg tactgcgact caaggtcgcg
     5521 gatattgacg gcgccattca gcacgatgga ggagtcgccg ggtgcgaaca ccggatcgct
     5581 gatgtcgaac tcgtcctcga tttcgcccac cagttgctcg aggatgtctt cgactgtgac
     5641 aacgccagca gtcgagccga attcgtcaac cactaccgcc agatgccgct tgcgctgctt
     5701 gaattccatc aacagatcgg ggagcggctt ggtctcggga accaccagca cctcgcgcat
     5761 cacgtgccgc actttgagat cgctggatcg gctctgcggt tggccagtca tcagctgccc
     5821 cagacgataa cgcaaccagc gcaaaaggtc cttggcataa agcactccga tgatgtgttc
     5881 gggtccccgc tcgggatcgt aaaccggcac ccgcgagtgg gtctcatccg cgactttttg
     5941 aaatgcttcg tccagcggca tgtcgcccgg caacgaaaag atgtccatcc gagggaccat
     6001 gatctcgcgt acgaacacgt tctccagatc gagtgcgcga tggatcatct gctcttgcgg
     6061 cgcttccagg atcccgatac gtcggctggc cgagacgatc agcttcaact cctcgggaga
     6121 atgcaccccg ccttcccgca cctggcgcgc gccgaaggcc ttgagaacca cgcgcgagga
     6181 ggcggtcatc accacaagga gcggggcggc cagcgtcatg aatacatcca tcggaccggc
     6241 aactgccaga gcgactgatt ccgtccgctg caacgctagc gacttgggca cgatctcacc
     6301 caggatcacg tgcatgtagg tgatcaggat gaacgcaaat ccgatgctga aggcgtgggc
     6361 ataaatgtgg gagtagggta gacggacgaa cagaggctcg agcacacgag ccattgccgc
     6421 ctcgccgatc cagcccaggg cgaggctgga gaccgtcact ccgaactgca ctgccgcaat
     6481 aaactcgtcg agcttctggt gcagcttctg gacggtacgg gcgccgatgc ggtgggcagc
     6541 aatgagctgc tgcagccggg tttccctgac gctgaccagc gcaaactcag ctgccacaaa
     6601 gaatgcgttt gccgcgacca gcaaaaccac cgccacgatc cgcagcacaa cgaaagtaac
     6661 catgaagctg aaatcttaac atcgggcggc tgggggtccc gacgcgacat gatgtaagca
     6721 tttctcgccc agcaacttac acccattgcg aaagcttgct accgtttccc atcccaattc
     6781 ggtctaacgc gatggaaaag ggcgatccgg ggcgggtttt tcatcatcgg gcacggaact
     6841 tctctccata ctggtacgta cacaccatca gaacggaatg ctacctagcg tcgggaggcg
     6901 gcagtggcca acggcaaaaa tcatagtaag aaaaagcgcc tagtcattat ttcaatagtg
     6961 gctgtggcag ttgtggcgct cgtcatcgtg gctgtagcgg caacccgcgg cggcaccaag
     7021 atcgatccca ccaaactggc gaaagtggaa aagggcgatc tcgccaagag tgtggtggcg
     7081 accggcaagg tcacgcctat cactaaggtc gagatcaagt cgaaggccag cggcattgtg
     7141 aagaagctct atgtcgacgc cggcgacacc gtgaagaagg gccaggtcct tgccgatctc
     7201 gaccgcgacg aaatcgaagc gcaggtgaac tcttcgcgcg cccagttgct ctcgtccgag
     7261 gccaaccaga agggcgccga ggccgatatg acgcgcgccg aggttgacgc ccagggcgtg
     7321 gatattccca ctctgcagcg cgcctacgaa cgcgcccagg agatggccaa aggtggcatc
     7381 gtgtccactt ccgctcttga tgatgcacag cgcatgtaca tcctggcggt caacaagcgc
     7441 gacgtggcga gggcgcaatt ggtggtcaac aaagccaagg ttgcccaggc aaaggccgat
     7501 gtacagaaga gcgacgccac cctcaagcag ttggaagagc agttgagcta caccaccatc
     7561 gtcgctccca tcgacggcat cattttgtcg cgcgacgtgg aaatgggcga cgcagtcagc
     7621 tccatcctgg tgatgggctc ggcggccacg ctggttatga ctattggcga taccagcgag
     7681 gtttatgtca agggtaaggt tgacgaaagc gatatcggca aggtgtatct gggacagccc
     7741 gcgcgcattc gggttgagac gttcaaggac aagactttca ctggccacgt gaccaagatt
     7801 tcaccgatgg gcgcggagaa ggacaatgtc accacctttg aagttcgcgt gtcgatcaac
     7861 aatccgggcg gcgagttgaa ggctgagatg acggccaacg cagagatcat cctcgaagag
     7921 cataagaacg tgctgatgat ccccgaaggc gcaattttgt acgacaagga caagaaggcg
     7981 tccaccgaag tgcccgatcc gaaaggcaaa gaaggcaagc gcaaagtggc gctgaatatt
     8041 ggcatctcca acggcgccaa gacggaagtg gtatccggcc tgaaggaagg cgatcaggtc
     8101 gtgttgcagc agtaacgttc catcctcctc caagggctgc cgccgacggc agcccacctt
     8161 tttgttcaac gtggttgcaa ttatccgagg cgaaatcatg aaaaaacatt tcatcgcgac
     8221 tcttcctgtc attctgctgg ttcctgttct tctgttggcg agagttgagg accagttcga
     8281 tcgcactctc ccggtttcag gctcggtgaa cctcgatctg accaccggtt caggcgacgt
     8341 gattatcaaa actggcgggt ccaaccaagt gatagttcat ggacgggtcc gcagcaacaa
     8401 cgagtggttt ggcgacaatg ctgatcgcct cgtccatagc gtcgcatcca atcccccgat
     8461 cgagcagaat ggcggcacga ttcgcatcgg ctacaacctg cccgaagacg ccaagcacca
     8521 tatctccatc agctacgaag ttaccgttcc ggccgacacc accgtgaatg ctggctctgg
     8581 atcgggcgag atttccgtgc aaggtgtgcg gtcgcccgtg aaactccata ccggctcggg
     8641 tgacatccgg gtgcaggacc tcggtcccca gtcccacgtc gatactggca gtggcagcat
     8701 ccacgctgag tcggttgcag ccccgttttc cgccgggacg ggcagcggag agattcaggc
     8761 tgacctgacc ggaagcggcg atgtggatgt gcacaccggc tcgggtggaa ttcgcattcg
     8821 cggcatcaaa ggcggcctga gcgcgcgcac tggcagcggc agtgtcgggg ccgacggaga
     8881 cgtaaaaggg ccgtggcaaa tacacagtgg ctcagggaac atcaagatgg cactgtcgtc
     8941 cggcggaggc ttcaatctcg atgtgcatac cagctcgggc tcgattcact cggaccttcc
     9001 tgtcaccatg cagggcacgc tgggtcggca cgaactgaaa ggcgcagtgc gcggcggcgg
     9061 tcccgaagtc gaagtttcga cgggttcggg ggatgttgat atccgctagg tttcgagtgc
     9121 tttccgtggc ggtcgctatt cgacgatcgt caccggatcg cccacccgga tcgtccccgg
     9181 cttaaccact gagcaatcca gcgccatggt tccgtcgaat tccttaacga tgcgaaacaa
     9241 cacattgcgg tcctgcttct gggtatcggg atcgtaggtg gtcatcacgc aacgagcgcg
     9301 aagctgagcc gcgtaaatct cgacctcgcc gatgcggagc tgcttgtctt cccacccccg
     9361 ctctgcgagt ccttcaaccc caccgatgag aatgttaaat cgaaatcgcc gcctatctac
     9421 cccgagtgca tcgagggccc cgtcggttgc aaccagcaat ggcaatacat cgaagcgctc
     9481 ggtgccggga acgtgcacca gttcggcttt ttcgccgacg gcctcctcga ccagcgcacg
     9541 cgcttcagga ctgtcccacg aatagccgtt gatggtggct gcgtgggtct cgggcgaaat
     9601 gccgccttgc aaaccgagca ggcggtgatg ggtccgcgcg gtgatcatcc ggcgacgcga
     9661 cgttgatacc acgacgatct cgcgatcgcc gcgaacgcca gtctgcagga cttcggcttg
     9721 ctgtaactgc tcgccccgca tgctcttcac cggacaccgc cacagttccg ctacgtgcat
     9781 gaatttccct cgccacaaac ctgcaaggct gcgttcatcg gatgccgccc acgtggaaaa
     9841 caatgcagga gttttgcgtc cgcttgactc ccgctctcca tccgactagc ttgcggtcat
     9901 ggctgaaccg cgcgttgtta ccttcaccag cgacttcgga ctcgacgatc ccttcgtcgg
     9961 aatcatgcat ggtgttgtgc tcagcatcca tcctgagacg cgcatggtgg atatctgcca
    10021 cgccatccca tcgttcaacg tgctcgatgc cgcgtggacg atcgcgcaag cctatcgttt
    10081 ctttccgccg cgcagtgtgc atgtggtcgt ggttgatccg ggagtgggta gcgcgcgaag
    10141 gccgattctc gcggagaccg acgactacat cttcgtggct cccgacaatg gagtgctgtc
    10201 gctcgtcgag gcgcgcgaac ccaagctgac ggttcggcac atcaccgccg accgctactt
    10261 tctccagccg gtcagccaga cctttcatgg gcgcgacatt ttttctcccg tcgccgcctg
    10321 gctcagcaag ggagtagcac ctgccgagtt tggccccgag gtcaccgact acgtgcgcct
    10381 gcccttgccc gcggttgagc atatcggcga aaacagtttg cgcggtgcgg ttctcaaggt
    10441 cgacaaattc ggcaacctca tcaccaacct cagtgaacaa gaggcgccgg cgttgtttgc
    10501 tgcaaccgcg ccaagtgtcc agttgctgat tgcgggccag accatcacgc gtgtatgccg
    10561 gtcttatgcg gaaggaggag atgacgaatt cttcgccatc cttggcagtt ctggctattt
    10621 agaaattgcc gcccggcaag cgtcggcggc ggagagactt tcggctggcg tgggcgctcc
    10681 ggtgggcgtg attctgcagg agtagctatg gctaagggtt cttcggtttc acgcgcgcat
    10741 aacgctccat ctcgccagtc caggtgagga aacgaattaa ctcccgtcgt tcgcgccaac
    10801 tgaatcccca gaactcgagg aagccgatac tctgcatttt gagcccggta taagtttcaa
    10861 tgcgccagcg caagtaggaa cttcgccacg gcgtcaggtg atgtccgcgg gtcgcgttcc
    10921 acagaaaacg aaggatgtag ccaatgtgct tcaatgcttt acgaagctgg cgtcagccgc
    10981 tgattgccgc cttcgcatga ctagtataac ggcacctcac cgcggcgttt gcgatgaacc
    11041 cacatgctaa actcggctcg gagttccatg accaacgaca cgtcgcgaca ggccgccgaa
    11101 gaccgctact acgccgcgct cgaccattac gctgaaggca gactgaccga ggcgattgcc
    11161 gattacgagg cagccatcgc taccgatccc acattcctcg atgccatgca cggactggcg
    11221 cgcgcctatc aggatgccga gcagttcgac caagccatca cggtcgccaa tcgcatcact
    11281 gaactcgatc ccgacgacgt acttgcccac accagcctgt cgattcttta tcagcgcaag
    11341 ggaatggttc ctgaggcgga agctgaggcc aacaaggccc gcattctcgg ctggaagcag
    11401 caactcaaac agggcaaaac gtcgcagcct tgacttctcg ggcccactcc gcgcgcaaga
    11461 ccgcagacaa acttcggcat gtctcgtcac cggcgcaaac cgttcgcgcg atttatcgca
    11521 aactatcgcg cgcgtgggga ccgcagcatt ggtggccggc ggaaacgccc tttgaagtga
    11581 tcaccggcgc catcctgacc cagaacactt cgtggacgaa cgtggaacga gccctgtcga
    11641 agcttcgtga ggctggcatg ttgagcgtgg agggcctcag tcaattgcca ttggacggac
    11701 tcgagcaact ggttcgctca tcgggctact ttcgccaaaa ggcgctacga ctgaaaagtt
    11761 tcattgcgtt tttggacgaa aaccatggcg gatcgcttga ctccttactt gcgactccca
    11821 ccgagcagtt gcgggaggaa ctgctagcac agaaaggcat cggccccgag acagctgact
    11881 ccattcttct ttacgcggga caccacccga tcttcgtggt cgacgcctac acccgccgcg
    11941 tactggagcg tcacgacgcg gtagccgccg atgctaagta cgatgaaatt cgcgctttgg
    12001 tcgagcacgc attgcagagc gaaaaaccgt cgccagcggt tgcgcccggc tcgctcgacc
    12061 agcaacgtcc tgcagtccat ccgccgtcag ctatgagcac cgcgccgcgt gccctctccg
    12121 ctcaggtctt caacgaaatg catggcctat tcgtgcagtt ggggaagcac tattgtcaca
    12181 agcaacagcc cgactgtgat gtatgtcctc tgggcgcgat gctttctcat ccagtcatcc
    12241 cgactctgac acgcaactcg cagcaacgat cgtccggcaa taaacggcga gcctcaaaaa
    12301 agccagccaa atcgcggtta taatcagact cggccctgaa accggcaatt tcctggtcta
    12361 tgaagttagg tgcgctcagc aatcggcgtt acctaagccg tcatgtcgcc cgtcacacct
    12421 ccaaaccggc cggaccacaa gcgcagccgc actcccgcga ttgtcggact gtcgatcgca
    12481 gttttcctgt tgttcgcaat tatcttttcg caagccacct tcaacctgac gttcctgcag
    12541 cccgatacca gcgaagagac gctgatcttt gccgcgcttt ccgcactcat ctttctcctg
    12601 ttcgtggccc tgacgtttgt gctgctgcgc attcttctgc ggctttacgc cgaacgcaag
    12661 acaggcgtca tgggatcgaa gctgcgcagc cgcatggtga tgggcgcgtt gctgctgtcc
    12721 ctgggtccgg tcatcttctt gttcctgttt tcctacggtc tgatcaaccg ctcgatcgag
    12781 aaatggttct cgcgtcccgt ggaagacgtg caataccgca ctggcgtggt ggctgcgctc
    12841 ctgaccgact acgcggccga caatgcccgg gtcgaagccg aacgaattgc gtcatcgccg
    12901 gccgccaaaa agtcgtttga gaccgacaac ttcacgctgg tgatggaaga gtttcgccgc
    12961 agcgaggtca gcctgcaggg cggattcgcc tttgcggttg ccaatgaccg cgccgaggcc
    13021 agctttcacg ctccccagcc gtggccgctc ttgcatgacc gcttgcccat gaaggaatcg
    13081 gcggagaaca agcccaagtc cttcgatatt ttgggaaaga cctacgtgct gggccgggcc
    13141 gttgtcggac ccggagcgca gatcctcgtc gccatgccac tgcccgccaa ctactccctg
    13201 gcattgcgtg acattgagca agcccagcag cagtaccagg aactgcgcaa gcagcgccgg
    13261 cgtctgcgcc gcacctatct cggctacata ttgatgctca ctgtcggcac tctgtttgct
    13321 tcgacgtggc tggcgttgta cctgtcgaaa atggtgaccc gcccgctggt tgccctggcc
    13381 gaggccacac aggagatttc acggggccac ctcgattacc gcgtggacgt gcaagtcggc
    13441 agtgaaatcg gacaactggt gaattcgttt aaccagatgg cggctgacct ggaagccagt
    13501 cgcgccagca tcgaggcctc gcgcgccgat ctggcggatg tcaacgttca gatcgagcag
    13561 cgcaaccgcc atatcgagac catcctggaa agcattccga ccggtgtgct gtcgctcgac
    13621 gcatcccgcc acgtggttca catgaatggt gccgtcgagc ggttgctcca tctcgatccc
    13681 tcgccggccc aggcggccag gacgctggag gaactgctcc ctgatgacgt gactgaggac
    13741 cttgagcatc tcatgcgcaa agcggaccgc atgggtacga cgaccagcca gatggagatt
    13801 gttacgtccc gcgtcaatct caacgtcgcg gtcaccgtgg cgtcactcga ttcacccgct
    13861 cgcgtgcagg aaccattagc gcagcgcatg ggatatgtgg ttgtgctgga agacatgacc
    13921 gacctgctcc gcgcccagaa gcaaactgcg tggagcgaag tggcacgccg cgttgcccac
    13981 gaaatcaaga acccgctcac tcccatcgca ctgtcggcag agcgcattcg ccgccatctc
    14041 gaaagaggag cgcctcccga tgcggcttcg cttaaaatca ttcgcaactg tgtcgatacc
    14101 atcggttcgt cggtgcagac ggtgcggacc ctggtggacg aatttgccac cctggcgcgc
    14161 ttcccggcgt cgcggcccat gccctccgac atcaacagca ttgtcgagag tgctttgctc
    14221 atgtttgacg gccgcctcag tggcatccgg gtgcagaaga ttcttgcctc aggactgcct
    14281 ccagttcacg ctgatcccga cgccatcaaa cgcgcgattg ccaatctcgt cgacaacgcc
    14341 gcggaggcga tgcagggatc gatggtcaag gagatcgtga tatcgactgc gctcgcagaa
    14401 gaacgtgatt cggtcgaaat cgtattgtct gacaccgggc acggcgtgac tcctgcgctg
    14461 aaagagaagc tcttcctgcc ctatttctcg accaagaagc gtggcacggg cctggggctg
    14521 gcaatcgtca gccgcattgt cgaggaccac cacggctcca ttcgcgtgga ggagaacact
    14581 cccatcggga cccgctttat cgtagagtta cctgtggcgt cggcgatggc ggtggagccg
    14641 tccgaacaac atgcacacca ttctgatcgt ggatgacgaa tcggaaatcc gcagttcgct
    14701 gcaaggcgtg ctggaggatg agggctacaa gaccgtgctg gcggagagcg ccgaggaatg
    14761 tcttgccacg ctcgcgcgca gggcggtcga tgtggtcctg ctcgacatct ggctacccgg
    14821 c
//
LOCUS       DASRKK010000423        13003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1401640, whole genome shotgun sequence.
ACCESSION   DASRKK010000423 DASRKK010000000
VERSION     DASRKK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401640"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="VFC15_09875"
     CDS             complement(<1..1967)
                     /locus_tag="VFC15_09875"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZM10505.1"
                     /translation="MNKELYVSSTPHETKVALVEDDQLAEVFFERDNEYTLAGSIYKG
                     RVTRVLPGMQSAFVEIGLERDAFLYVSDFLELSDEEDSEEFGDIPAPHTTVNLSPQSP
                     NPGMQSAIADLPVDEEAEFLAAVDGVSPVETDDETESGESSATSAEDEKSGEGARRWR
                     GRRRRGGRRGGRDERPERTEPKVEAAKAEEQPIEAVEPAAEVPAEHRPEPRSDSRYDS
                     RQDSRSSSRGENRSRSDFGPPSGYKPMVMPGESISKYRNLPQQPAAETVVVTEELDAA
                     IDPVENVAAAPVEDFVAPVEPQHVEEVAFEETEEVVEPRVEPHVSAATEEDEVEEETE
                     SATPEQTHTTSFVVSDPEVQPIDVPNVDVQNVDAQSSAAHEEAAVVSVSDAPQPHSHI
                     EPSEYGTIDIVPDATEGEQLPSDSQTESQFTSGFQQHSAPSEAPEGRLLEHETLEDDE
                     MEFHPMPENIEALSEVAGDLELEMEEETLDSSDGYSDRWIPKVADVDEDEVVAAEERG
                     ETRFAAGGEEIEEDEEEEPGDETNGHAELRAAPPTAGYQQRQTERQGFNRRNDRGRRG
                     GGGGGRRNNVRTGPHSHGPQPLISDLLKEGQEILVQIAKEPIGKKGARITSHIALPGR
                     FLVYMPTVNHTGVSRKINSEEERPRLKRIVT"
     gene            complement(2390..3475)
                     /gene="rodA"
                     /locus_tag="VFC15_09880"
     CDS             complement(2390..3475)
                     /gene="rodA"
                     /locus_tag="VFC15_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZM10506.1"
                     /translation="MSRYVSYRDFDWVLLALVLLICSIGVVEIYSATLNTKFMGVHIK
                     QIYWVLGGVALMFAMSIVSYQALIERVPVFYIVSIASLLAVEIFGKKYLGARRWVQLG
                     SFHFQPSEWVKLVLILAMAKYFADNKGECDTQDIVKAGALVGIPMLMVLAQPDLGTAL
                     TYLPIAIMGLFLGGMKAKHGLVIVLIATLLAPVAWHVLKPYQRDRLTTFIEPEADPQH
                     KGYQLIQSLVAVGSGGIFGKGTAQGSQTQGQFLPVPHTDFIFAAYAEEHGFIGAMGLL
                     LLYFIVLMRLIQNAQTAPDRAGAFVVMGVVAVLAFHILVNVGMVVGFMPVTGIPLPLM
                     SYGGSSVLFMFLALGIVMNVRMRRFVN"
     gene            complement(3472..5670)
                     /gene="mrdA"
                     /locus_tag="VFC15_09885"
     CDS             complement(3472..5670)
                     /gene="mrdA"
                     /locus_tag="VFC15_09885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZM10507.1"
                     /translation="MFGREEKVSPFRLTAVQYVLLAIFLFLAFGLWRLQVARSDYYSS
                     LAEQNRIKQVPILAPRGKLLDREGRIIVDNYPSFSVLLLRDQNRNLEIDAEKIAEGLH
                     IPPDELRTRLKRMAAVPGYQPMFLKDDITPDELAFIESHRADLPELDIITVHRRLYPK
                     GGFMAHVIGYVGQVSEEMLTQPQWELYNPGDVVGMSGVEQYYNDVLMGKNGSRQVLVN
                     SRGKEVGTLSDVPAIPGKQLKLTIDLDLQIAAEEALEGKPGAIVAMDPRTGEILAMVS
                     RPAFDPNAFAVRVSRDEWNRLMTDPGKPLLNKAIQAQLAPGSVFKIIMATAGMQEGIA
                     QDLVVSCGGGKSFYGRYFKCWIAARHSTHGSVNITKAIYQSCDSYFYTLAEKLGISRI
                     AKYATMLGLGQKTGVDLPQEVSGVMPSEEWKIKNYKQKWYAGETISVGIGQGAVATTP
                     IQLARAAGAITTGGALRRPHIAFPEQFPPEFKQVAEYNDEIRIPIDEKNWVTITDAMA
                     QVVSGIGTAGRAAVPGIDIAGKTGSAQTVSNDLKKKMGASEKDLYKDNGWFVGVSPRR
                     NPEIVVACLFEGGEHGALAAAVATKVIKAYVEKQRNRQTQVAKAAAGNQAEVAAVWHD
                     GDAKPGDNKLQGGHFTVPLDAKVKPVAAAPGMSQNGGSAPASDLQATESHPEMASPEP
                     ETPPQAQPTQPPLRQEAAPDGQPATEPNPKKPAAKPAIAPAATAVRGAQP"
     gene            complement(6155..6697)
                     /gene="mreD"
                     /locus_tag="VFC15_09890"
     CDS             complement(6155..6697)
                     /gene="mreD"
                     /locus_tag="VFC15_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZM10508.1"
                     /translation="MSTRSMPFRGSFTSREQVSVYEFSWLATVLIPLIAIFIQAFLPT
                     KIRWLPIIDLPMMVVIFFAVARRNPISGCVTGAIIGLLQDLLGGPAHPIGMYGITNTI
                     IGYMASSLGVKIDIENPGSRFLIIYGFFVIHQALYYGVGHGMLRQPLEWSWSHVAISA
                     LANAVVGVLVFNLLDRFKQR"
     gene            complement(6694..7980)
                     /gene="mreC"
                     /locus_tag="VFC15_09895"
     CDS             complement(6694..7980)
                     /gene="mreC"
                     /locus_tag="VFC15_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZM10509.1"
                     /translation="MDTLLGRYKNLIVLAAILFAQIIALAMQVRRPAAEGETRLIRVW
                     ATAAVTPFEEAIVHTQKWAHDKWSGYVYLRHVDQENQQLRAEINRMKLEQARLAEDAN
                     MARRVQTLLAFKEQYLESTVAAQVIGTSGSEQSRVLYIDKGSSDGIKLDMAVITPTGI
                     VGKIVQVFPGSSQVLPINDQLSGVGAALKALRLQGILKGAANGTTTLQYIMSDEPVKL
                     GEEVITSGGDRIFPKGLPVGTVASVEPGKDLFLNIRVIPSARLDRLEEVLVVTKIQDK
                     LPDAKDLGPIRASDILSERLPGVPVTQTVDAAGNAAGTASGAGNPTQPNGAPSATAAK
                     PGAATNSGQQNAAGSSGTAKPGAAGSSVPKATATPASNPANGGANSAPKPNLGAGTTP
                     GASAPKPVRKAPATVSPDANPTQPATPPSAQENPPR"
     gene            complement(8005..8484)
                     /locus_tag="VFC15_09900"
     CDS             complement(8005..8484)
                     /locus_tag="VFC15_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10510.1"
                     /translation="MSSISGRPKAFDTILLGGLIAGALDGLDAVVYYGWAFAVTPTAL
                     FQHIASGLLGSRSFGGGWATTVLGVACHFTIAIGAAAVFYAASRAIPFLGQKPWISGP
                     AFGICVYVVMHYIVVPLSAVAPRKVPMAGLELVDQLFSHMFFVGLPIALMARRSARR"
     gene            complement(8532..9533)
                     /locus_tag="VFC15_09905"
     CDS             complement(8532..9533)
                     /locus_tag="VFC15_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZM10511.1"
                     /translation="MFSSDLAIDLGTANTLVYARGKGIVVNEPSIVAINKNTGEVEAV
                     GKEAKEMLGRTPGNIVAIKPMKDGVIADFKVTEKMLNYFIQKAHNRKMLVHPRIVIGV
                     PSEITQVEKRAVMDSAYRAKASEVHLVEQAMVAAIGAGLPITEPSGNMVVDIGGGTTD
                     IAVISLSGIVYSRSVRMAGNQMDEAIMNFMKRKYNLLIGERTAEQIKLEIGSAYPLEK
                     PLTMEIKGRNLIEGVPKTITIDDSEIREAVSECVSTIMNAIRVALERTPPELSADISD
                     RGIVLTGGGALLKNLDKRIREETGLPVSIADDPLCSVVLGTGKMLSDFKLLRKISID"
     gene            10136..12844
                     /locus_tag="VFC15_09910"
     CDS             10136..12844
                     /locus_tag="VFC15_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HZM10512.1"
                     /translation="MFAKICSFISLFAVACLGSVSLAATLQKQTVSLNSAWEFRQVPA
                     TTPTQAPAAATNDATQWRPAVVPGSVHLDLLRNKLIPDPFYRDNEAKLQWIENADWEY
                     RTTVQAAPALLAQKNIDLVFEGLDAYSKVYLNDKLILTADNMFRQWRVNVKADLKPGA
                     NTLRVVFPSPIKAASEIAANDPWRERTHTEPKTYIRKAAYEFGWDWGPRFVTSGIWRP
                     VRLEAWDTARISNVHIRQLDVSNAVAHLLAEVEVTASEAGNATISLEYGVTVKHSDAT
                     RTVELQPGVNHVTLPIDISNPQLWYPAGYGAQPIYKFQVQLKSAKQTLDEATAKTGLR
                     SIVLRREPDQWGRSFEFVVNGIPVFGKGADVIPFDSFPTRVTTQQYRQVLQSAVDANM
                     NMIRHWGGGYYETDEFYDLCDELGIMIWQDFMFGNDWQPGTYDWKLNVAQEAEQQLKR
                     LRNHPSIVIWCGNNETEAALAWKDRDKLSPDVRFQMWQDYVSTFSGILAAAVNRLAPE
                     TPYWPSSPSADYEELSDKYQSGDMHDWSVWHGRVPFADYEKHNPRFMTEYGFQSFPEM
                     RTVETFTIPEDRLNIFTPVMLAHQKNAAGNAIIRDYMLRDYPQPKDFASFLYASQVLQ
                     AEGIKIGAEHLRRNRPRTMGSIYWQLNDCWPVASWSSLDYYGRWKALHYYARRFYAPV
                     LVSPHQEEGNVAVYVVSDKIAPMVATLRVRILGFDGAVLSDKSQTIQVAPLASKVYLT
                     IPMLDITNLPNADLGKIFAATDLLVDNKVISTNTLFFVPTKDMQLPQAKIESKLTAAA
                     PSNVGAPPSRPAQRDDRVGSNPVATESNYTLHLSSPVLAESAYISFGNLDAKPSDNYF
                     DLLPGQPVDLTITSAASLDELKTNMRVISLTDAFAPHEATVSAGTN"
BASE COUNT         2558 a         4141 c         3563 g         2741 t
ORIGIN      
        1 ctggtgacga tgcgcttcag gcgcgggcgc tcttcttcgg agttgatttt gcgcgaaacg
       61 ccggtatggt tcaccgtcgg catgtacacc aggaaccgtc ccggcagagc gatgtgactg
      121 gtgatgcgag cgcccttctt accaatcggc tccttggcga tctgcaccag gatctcctgc
      181 ccttctttca gcaggtcgga gatcagcggc tgaggaccgt gcgagtgcgg gccggtacgg
      241 acattgttgc ggcgtcctcc gcctccacct ccccggcgac ctcgatcgtt gcggcgattg
      301 aatccctgac gttccgtctg gcgttgctgg tacccggccg tcggcggcgc tgcccgcagt
      361 tctgcgtggc cattcgtttc gtcgcccggc tcttcctctt cgtcctcctc aatttcctcg
      421 ccgccagctg cgaaccgggt ttcgccgcgt tcttcggcgg cgacgacttc atcttcgtcc
      481 acgtcagcaa ccttgggaat ccaccggtcg gagtagccgt cgctgctgtc gagtgtctcc
      541 tcctccattt caagctccag gtcgccggcg acttcgctca gcgcctcgat gttctccggc
      601 atgggatgga attccatctc gtcgtcttcc agcgtttcgt gctccaacag gcgtccttcc
      661 ggggcctcgg aaggcgcgct gtgttgctgg aatccggaag tgaattgcga ctcggtctgg
      721 gaatcggaag gcaactgctc tccctcggtc gcgtccggca cgatatcgat cgtcccgtat
      781 tcggagggtt cgatgtgcga atgaggttgc ggagcatcgc tgaccgaaac cactgcagct
      841 tcttcgtgcg ccgcactgct ctgcgcatcg acgttctgta cgtcgacgtt cggcacatcg
      901 attggctgga cctcaggatc ggagaccacg aacgacgtgg tatgcgtctg ctccggtgtg
      961 gccgactcag tctcttcttc gacttcgtct tcttctgtgg cggcagatac gtgtggctcg
     1021 acgcgcggct cgaccacttc ctcggtctct tcgaaagcca cttcttccac gtgctgcggt
     1081 tcgacgggtg cgacgaaatc ttcgaccggc gcagccgcga cgttctccac cggatcgatc
     1141 gccgcgtcca gttcttcagt cacgacaacg gtttcggcag cgggctgctg gggcagattg
     1201 cggtacttgg agatcgactc tcccggcatc accatcggct tgtatccgga aggagggccg
     1261 aaatcggaac gcgatcggtt ttccccgcgc gaactggagc gagaatcctg gcgcgagtcg
     1321 tagcgcgagt ccgaacgcgg ctccggacga tgttctgccg gaacttccgc cgctggctca
     1381 accgcttcaa taggctgctc ttcggcctta gcggcttcaa ccttcggctc cgtgcgttca
     1441 ggacgctcgt cgcgaccacc gcgacgtcca cctcggcgac gacggccacg ccagcggcgg
     1501 gcgccttccc cggacttctc gtcttcggcg gaagtcgccg agctctctcc cgattcagtt
     1561 tcgtcatccg tttccacggg tgagacaccg tccacggccg ccaggaattc ggcttcctcg
     1621 tcgactggca gatctgcaat tgcagactgc attcccggat tcggtgactg cgggctcagg
     1681 ttgacagtgg tgtggggcgc gggaatgtct ccgaattcct cggaatcttc ctcatcgctt
     1741 aactcgagaa agtcggatac gtaaaggaag gcatcgcgct ccagcccgat ttccacgaat
     1801 gcggactgca ttcccggaag tacgcgggtg acacggcctt tgtagatgga tcctgcgagg
     1861 gtgtattcgt tgtcccgctc gaaaaatact tcggcgagct gatcgtcctc caccaaggcc
     1921 actttggttt cgtggggtgt cgaggacacg taaagttctt tgttcatgga cgctccggtg
     1981 gaccggcgcg cgcgctcagg aggcgaacaa gaaagtaatc aacggtgatc aggccgcgga
     2041 cgcgcacggg atctcgcccg gccgtgttcc cagcctgatg ttggctacag caatctgtgg
     2101 aatcgtctgc accgttatta ggaaagtcgt cggaactttg tgaccacacc ttccgaccgc
     2161 ggaaggggtc gttacccgcc tctgacgact taacctgcaa tcctgtgcgg cctttttcac
     2221 ttgcggccga tacctgccct cgtacgtgag ggcgctcgta aaatttttgg tcactcttcg
     2281 aagacccgaa tccttgtttc ggaaagtctt cccggcgagt gaaaccggtt gctgccgacc
     2341 cgcgtcgcat tggcgcgtaa tctgacgaac agcaaaaact ttggttacct cagttcacaa
     2401 accggcgcat tcggacgttc attacgatcc ccagcgccag gaacataaac agtaccgacg
     2461 atcccccgta actcattaac ggcaagggga ttcccgtgac cggcatgaac ccaaccacca
     2521 tgcctacgtt gactaggatg tggaaggcca aaaccgccac cactcccata accacgaagg
     2581 caccagcccg gtcaggggcc gtctgagcgt tctgaatcag acgcatcagc acgataaagt
     2641 atagcagcag taggcccatc gcgcctatga atccatgttc ctcggcatag gcagcgaaaa
     2701 tgaagtcggt atggggaacc ggcaggaact ggccctgcgt ctggctgcct tgggcggtcc
     2761 ccttgccgaa aatcccgcct gaccctaccg ccaccagcga ctgaatcaac tggtatccct
     2821 tgtgctgggg atcggcttcc ggctcgataa acgtggtcaa acggtcgcgc tggtaaggct
     2881 taaggacgtg ccacgcgacc ggggccaaca gggtcgcgat cagcacaatg accaggccgt
     2941 gcttggcctt catcccaccc aggaacaggc ccatgatcgc gatgggcaga taggtcaagg
     3001 ccgtccccag gtcgggttgc gccagtacca tcagcattgg gatacccacc aaggctccgg
     3061 ctttcacaat gtcctgcgtg tcgcactcgc ccttgttgtc ggcgaaatat ttggccatcg
     3121 ccaggatcag caccagcttc acccattccg atggctgaaa gtgaaacgag ccgagttgca
     3181 cccatcgccg ggcgcccaga tactttttgc cgaaaatctc taccgccagc aacgatgcga
     3241 tcgacacgat gtagaacacg ggcacccgct caattaacgc ctggtagctg acgatgctca
     3301 tggcaaacat cagggccacg ccacccagca cccaatagat ctgcttgatg tgcacgccca
     3361 tgaacttggt gttgagggtt gcgctataaa tctcaaccac gccgattgag caaatcagca
     3421 gcaccagcgc cagcaagacc cagtcgaagt cacgatacga gacgtaacgg ctcatggctg
     3481 cgcccctcga accgcagtcg cagcaggcgc aatcgcaggc ttggctgccg gtttcttggg
     3541 attcggctcg gtcgcaggct gcccatcagg cgcggcctct tgccgcagcg gaggctgcgt
     3601 cggctgcgct tgcggaggag tctccggttc aggactggcc atctcagggt gcgattccgt
     3661 cgcctgtaaa tccgacgccg gcgcggaccc accgttctgc gacatgcctg gcgctgccgc
     3721 taccggcttc accttcgcat ccagtggcac cgtgaagtgt ccgccttgca acttattatc
     3781 gcccggcttg gcgtcgccgt catgccagac ggccgccacc tcagcttgat taccggccgc
     3841 agctttcgca acctgcgttt gccggttgcg ctgcttctcc acgtaagcct tgattacctt
     3901 ggtcgctacc gctgccgcca acgctccgtg ctcgccgccc tcaaacaaac aagccaccac
     3961 gatctcggga ttgcgtcgcg gactcactcc aacgaaccat ccgttgtcct tgtagaggtc
     4021 tttctcgctg gctcccatct tcttcttcag atcgttggaa accgtctgcg cgcttcccgt
     4081 cttaccagca atatcaatcc ccgggactgc tgcccgtcct gccgtcccta ttcccgacac
     4141 cacctgcgcc atcgcgtccg tgatggtcac ccaattcttc tcgtcgatcg gaatcctgat
     4201 ttcgtcgttg tattcggcca cctgcttaaa ttccggcgga aactgttcgg ggaacgcgat
     4261 gtgcggacgc cgcagcgctc caccggtggt gattgcgcca gccgcccgtg cgagttgaat
     4321 tggtgtggtc gccactgcgc cctgtccgat gcccactgat atagtttcgc cggcgtacca
     4381 cttttgcttg tagttcttga tcttccactc ttccgacggc atcacgccgg acacctcttg
     4441 cggcagatcg actcccgttt tctgtcccag gccgagcatg gtggcgtact ttgcaatacg
     4501 gctgatacca agcttttctg ccagcgtgta gaagtacgaa tcgcaggact ggtagatcgc
     4561 tttggtgata ttcacgctgc catgcgtgct gtgcctcgcg gcaatccagc acttgaaata
     4621 gcgcccgtaa aagctcttgc cgccgccgca actcaccacc aggtcctgcg caatcccttc
     4681 ttgcatgccg gccgtcgcca tgatgatctt gaacaccgaa cccggcgcca actgcgcctg
     4741 gatggctttg ttcaacagcg gtttgccggg atcggtcatc agccggttcc actcgtcgcg
     4801 gctgactcgc accgcaaacg cgttgggatc gaacgccgga cggctcacca tcgctagaat
     4861 ttctccggtg cgtggatcca tggccacaat cgctcccggc ttgccttcca gtgcctcttc
     4921 ggccgcgatc tgcagatcga gatcaatcgt tagcttgagc tgcttgcccg gaatcgccgg
     4981 tacgtcgctt agcgtgccga cttccttgcc tcggctgttg accagcacct ggcgcgagcc
     5041 gttcttgccc atcaacacat cgttgtagta ttgctcgact cccgacatgc caaccacgtc
     5101 gcccgggtta tagagctccc attgcggctg ggtcagcatc tcttcgctca cctgcccgac
     5161 atagccgatt acgtgcgcca tgaagccgcc cttgggatac aagcggcgat gcacggtgat
     5221 gatgtccagt tccggtaaat cagcacggtg cgattcgatg aaggccagtt cgtcgggagt
     5281 gatgtcgtcc ttcagaaaca tcggctggta gcccgggacc gccgccatgc gcttcagtcg
     5341 cgtccgcagt tcgtcgggag gaatgtgcag cccctcggca atcttctccg catcgatctc
     5401 gagattgcgg ttctgatcgc gcagcaggag cactgaaaac gaagggtaat tgtcaacgat
     5461 gatgcggcct tcgcggtcca gcagcttgcc gcgcggcgcc aggatgggta cttgcttgat
     5521 gcggttctgt tcggccagcg aactgtagta atcgctgcgc gcaacctgca gccgccacaa
     5581 gccgaatgcg agaaacaaga aaatcgccag cagcacatac tgcactgccg tcagccggaa
     5641 tggggaaacc ttttcctcgc gaccaaacat ctgttactgc atctctcgat cgaggctcgg
     5701 caacatttgc cgaatcctga aactaagccg gtccgcgcgc gccgctaaac tcacggttgc
     5761 gccgacgcgc atggtcagtc caaattgtgg ttcggcaata ctcacgcagg tgggggaatg
     5821 gcgcaagtcc ctctgcctat tctaatccga gtcgccgccg cgcgggattc aaaaaaagtc
     5881 gtgtaactcg agtccagcgc ctcttagccg tttgactgtg ggtgagttgg gtggagcacc
     5941 gcttcagcgg tgcattgccg cttgcttaag ggctggcttt agccgctgag gtaagaaaag
     6001 actttacctc aggcgctaag gcgctttcgc acaccacctc gtcatgatgg acccctgaaa
     6061 gcgtaatcca accacccgcg atataccgtg tgtgaagttc gtctagttgc ggtatgacga
     6121 gcgcccagcc tatgcgtcgc tgtgaagtca acgctcatcg ttgtttgaaa cggtccagca
     6181 ggttgaacac cagtacccca actacggcat tggccaatgc ggaaatcgcg acgtggctcc
     6241 aactccattc cagcggctgc cgcagcatcc catgtcccac tccgtagtac agcgcctggt
     6301 ggatgacaaa gaacccatag atgatcagga atcgcgatcc cggattctcg atgtcgatct
     6361 tcacccccag cgacgaggcc atatatccga tgattgtgtt ggtgatgccg tacatcccga
     6421 tgggatgcgc cggaccgccc agcaggtctt gcagcaggcc gatgatggcg cccgtcacgc
     6481 agccgctgat cgggttgcgc cgtgccaccg caaagaagat caccaccatc atcggcagat
     6541 cgatgatggg cagccagcgg atcttggtcg gcagaaacgc ctgaatgaat atggcgatca
     6601 gcggaatcag gacagtggcg agccagctga actcatagac cgaaacttgc tcgcgcgagg
     6661 tgaagcttcc gcgaaatggc atcgatcgtg tgctcatcgc ggcgggttct cctgggcgct
     6721 gggtggagtt gccggttgcg tcggattggc gtccggagaa accgtagctg gcgccttgcg
     6781 caccggcttc ggtgccgagg caccgggcgt tgtgccggcg ccaagatttg gtttgggagc
     6841 tgagttcgcg ccgccgttgg cggggttgct tgccggcgta gcagtagcct taggcacact
     6901 tgaacctgcc gccccaggtt tcgccgtgcc cgaagaccct gccgcatttt gctggccgga
     6961 attcgtcgcc gcgccaggct tcgctgccgt cgcacttggc gctccattcg gctgcgttgg
     7021 attgccagcc ccactcgcag tccccgctgc attccccgcc gcgtcgacag tctgcgtgac
     7081 cggcactccc ggcagccgct cactcaaaat gtccgaggct cgaatcggtc ccagatcttt
     7141 cgcgtcaggc agtttgtcct gaatcttggt gaccaccagc acctcttcca gccggtcgag
     7201 gcgggctgac gggatcacgc gaatgttcag gaacaagtct ttccccggct ccacggaagc
     7261 caccgtgccc accggaagtc ccttgggaaa aatgcgatcg ccgccgctgg tgatgacttc
     7321 ttcacccagc ttcaccggct catcgctcat gatgtactgc aacgtggtgg tgccgttcgc
     7381 cgcgcccttc aggattcctt gcagccgcaa tgccttcagc gctgcaccga ctccgctcaa
     7441 ttgatcgtta atcggcaaca cctgcgacga tcccggaaac acctgcacaa tcttgcctac
     7501 gatgccggta ggcgtaatca ccgccatgtc gagtttgatg ccgtcgctgg aacccttgtc
     7561 gatgtacagg acgcgtgact gttcgctgcc gctggtgccg atcacctgcg ccgcgacggt
     7621 gctttctagg tattgttcct tgaacgccag cagcgtctgg acgcggcgcg ccatgttggc
     7681 atcttccgcc agccgcgcct gctccagctt catgcggttg atttcggccc gcagttgctg
     7741 attttcctga tcgacatgcc gcaagtaaac gtagccactc cacttgtcgt gcgcccactt
     7801 ctgagtgtgc acgatggctt cctcaaaggg agtcacagcg gctgtggccc atacgcggat
     7861 caggcgggtt tcgccctctg ccgccggacg ccgcacctgc atcgcgagcg caataatctg
     7921 ggcgaacaag atggccgcca gcacgatcag gttcttatag cggccgagga gagtgtccat
     7981 aacgttgcca gtcttagaag tgcgttacct gcgcgccgat cgtcgcgcca tgagtgcgat
     8041 gggaaggcca acaaaaaaca tatgcgaaaa aagttggtcg acaagctcca gacctgccat
     8101 ggggaccttc cggggcgcca ccgccgacaa cggcaccacg atgtagtgca tcacgacata
     8161 cacgcagatt ccgaatgccg gcccggaaat ccatggtttc tgccccagga acggaattgc
     8221 ccggctggca gcataaaaaa cagccgccgc tccaattgca atcgtgaagt gacatgcgac
     8281 gccaagtacc gtcgtagccc agcctccgcc gaatgaccgg gatcccagaa gtccgctggc
     8341 gatatgttga aatagcgctg tcggtgtgac cgcgaaggcc caaccgtagt acaccaccgc
     8401 atcgaggccg tccaaggccc cggcgatcag gccgcccagc agtatcgtgt cgaaagcttt
     8461 gggccgtccg ctgatcgagg acattaccgc aaacatcctt attgaccgat tttctctggc
     8521 agccgatcca gctagtcgat cgaaatcttg cgcagcagct tgaagtcgct cagcatcttt
     8581 cccgtgccca gcactacgct gcacagcggg tcgtcggcga tcgacaccgg cagtcccgtc
     8641 tcttcgcgaa ttcgcttgtc gaggttcttc aacaacgcgc caccaccggt cagcacgatg
     8701 ccgcgatcgc tgatgtcagc cgacaactcc ggaggtgtcc gctccagcgc aactcgaatc
     8761 gcattcatga tggtcgaaac gcattcgctc acggcctcgc ggatttcgct gtcgtcaatg
     8821 gtgatcgtct tgggcacgcc ttcaatcaaa ttgcggccct tgatttccat ggtcagtggt
     8881 ttctccagcg ggtacgcgga gccgatctcc aacttgatct gctcggccgt gcgttcgcca
     8941 atcagcaggt tgtatttgcg cttcatgaaa ttcatgatgg cttcgtccat ctgattgccc
     9001 gccatgcgga cggaccgcga gtacacgatg ccgctcagtg aaatcaccgc gatatcggtg
     9061 gtgccgccgc caatatcgac caccatgttg ccgctaggct cagtgattgg cagacctgcg
     9121 ccgatggccg ctaccatcgc ctgctctacg agatgcactt cgctggcctt ggcgcgatag
     9181 gcagagtcca tgactgcgcg cttttccacc tgcgtaatct ctgacggaac gccgatcacg
     9241 atgcgtggat gcaccagcat cttgcggttg tgcgccttct ggatgaagta gttcaacatc
     9301 ttctcggtga ctttgaagtc cgcgatcacg ccatctttca tcggcttgat ggcgacgatg
     9361 ttgccgggag tgcgaccaag catttccttg gcctccttgc ctaccgcttc cacctctcca
     9421 gtatttttgt tgatcgcgac aatcgaaggc tcgttgacca cgatgccctt gccgcgggca
     9481 tacaccaggg tattggccgt tcccagatcg atggccagat cgctcgaaaa catgctgaat
     9541 agcgaacgca agtttccgaa gcgtgaggga cgattatgaa aaccgttgga tgacattacg
     9601 actgcttctt tctatgagga ccgtataaat atatgaatgc tgccacagtt atttcacact
     9661 tagtacgcgc atgctggcaa cccgatgcct tcatccacaa acataaagga tgacgaaagt
     9721 attgcaactc cagagtattg tcgttccgag gacaccgccg gcaggaccgg tcggcccgct
     9781 tgctccggac cgctacatca tcgcgactac acggcatatc gagtttgccc gctccgccca
     9841 tcaccgggga ccggttcgtc tcgaagcaca attgtacggg aaatctgtga ccactccgcg
     9901 tgaaaagaga gtaccacttg tgacgaaaac attgaaatag cggccgcctg ctactttggc
     9961 cgtaatcgcg ccgcgcaggc cgtctggcac gcacttcgcc gcattccgtc ccccgtcagc
    10021 acccgaaatt ccgcaatagt tccggcgaaa tcacagccct cgccacgctt ccttgctact
    10081 atttggcgac tcgagctgca ccgctttttg ctcggatcaa ataggagcca tccctttgtt
    10141 tgcaaagatc tgttctttta tttcgctgtt cgccgtcgcc tgcctcggtt ctgtttctct
    10201 ggccgcgacc ctccagaagc aaaccgtgtc gctgaactcc gcatgggaat tccgccaggt
    10261 cccggccacg actcccacgc aggcgcccgc cgcggctaca aacgacgcta cgcagtggcg
    10321 tccggcagtg gttccgggca gtgtgcacct cgatctgttg cgcaacaaac taattcccga
    10381 cccgttctat cgcgacaacg aagccaagct gcaatggatc gagaacgccg actgggaata
    10441 ccgcactacg gtgcaagcgg cgccggcgtt gctcgcgcag aaaaacattg acctggtatt
    10501 cgaaggactc gacgcctact ccaaggtcta tctcaacgac aagctcatcc tcactgccga
    10561 caacatgttt cgccagtggc gcgtcaacgt gaaggccgac ctcaagcccg gcgcgaatac
    10621 attgcgcgtg gttttccctt cgccgatcaa ggccgcatcc gagattgctg ccaacgaccc
    10681 ctggcgcgaa cgtactcata ccgagcccaa gacttacatt cgcaaggccg cctatgagtt
    10741 tggttgggat tggggaccgc gtttcgtcac cagcggtatc tggcgtcccg tgcgccttga
    10801 agcctgggac acggcccgca tctccaatgt ccacattcgc cagctcgatg tcagtaacgc
    10861 agtcgctcat ctgctcgcag aggtcgaagt caccgcctcg gaggccggca acgcgacgat
    10921 ctcactggag tacggagtca ccgtaaagca ttccgacgcg actcgcaccg tcgagcttca
    10981 gccgggagtg aatcacgtca cgctgcccat cgacatcagc aatccgcagc tctggtatcc
    11041 agccggatac ggcgctcagc ccatctacaa attccaagtc caactaaagt cggccaaaca
    11101 gacgttagac gaagctaccg ccaaaactgg actgcgttcc atcgtattgc ggcgcgaacc
    11161 cgaccagtgg ggacgctcct ttgagttcgt cgtgaacgga atccccgtat tcggcaaagg
    11221 tgccgacgtg attcccttcg acagcttccc cacccgcgtc accacgcagc aataccgcca
    11281 ggttttgcaa tcggcggtgg acgccaacat gaacatgatt cgccattggg gtggaggcta
    11341 ctacgaaacc gacgagttct acgacctctg cgacgaactc ggaatcatga tctggcagga
    11401 cttcatgttc ggcaacgact ggcagcccgg cacctacgac tggaagctca acgtcgccca
    11461 ggaagctgag cagcaactca aacggttacg caaccatccc agcatcgtca tctggtgcgg
    11521 caacaacgag accgaggccg cgctcgcctg gaaagatcgt gacaagctaa gtccagacgt
    11581 ccgctttcag atgtggcagg actacgtttc caccttcagc ggcattctgg ccgctgcggt
    11641 caatcgcctc gcgcccgaaa cgccctactg gcccagttcg cctagcgccg actacgaaga
    11701 actctccgac aagtaccagt ccggagacat gcacgactgg tcggtctggc acggccgcgt
    11761 ccctttcgcc gactacgaga agcacaatcc gcgcttcatg accgagtacg gattccagtc
    11821 gttccccgaa atgcgcaccg tcgagacctt caccattccc gaggatcgcc tcaacatctt
    11881 cactcccgtc atgctcgcgc atcagaagaa cgcagccggc aacgccatca tccgcgacta
    11941 catgttgcgc gactatcccc agcccaagga cttcgcctcg ttcctttacg ccagccaggt
    12001 gttgcaggcc gaaggcatca agatcggagc cgagcatctg aggcgcaatc gtccccgcac
    12061 catgggatcc atctactggc aactcaacga ctgctggccg gtggcctcgt ggtcgagcct
    12121 cgattactac ggacgctgga aggcactgca ctactacgcc cgccgcttct acgcgccggt
    12181 gctggtctcg cctcaccagg aggaaggcaa tgtagcggtg tatgtggtct ccgacaagat
    12241 cgcgccgatg gttgcaacat tgcgcgtgcg catcctaggc ttcgatggcg cggtgctgag
    12301 cgacaagtcg cagaccatcc aggtcgcgcc actcgccagc aaggtatacc tcaccatccc
    12361 tatgctcgac atcaccaacc tgccgaatgc tgacctcggc aagattttcg ccgccaccga
    12421 tctgctggta gacaacaagg taatttcgac caacacattg ttcttcgtcc ccacgaaaga
    12481 catgcaactg ccgcaggcaa agatcgaaag caagctcacc gccgctgccc catcgaatgt
    12541 gggtgcccca ccctcgcggc ccgcgcagcg ggacgatagg gtgggatcca accccgtcgc
    12601 gaccgagtca aactacacgc tgcatctctc ctcgcccgtc ttggcggaga gcgcttacat
    12661 cagtttcggc aacttggatg cgaagccatc ggacaattac ttcgacctgc tgcccggcca
    12721 accagtcgat ctgaccatca ccagcgcggc gtcgcttgac gaactaaaga cgaacatgcg
    12781 cgtgatctcg ctgaccgacg ccttcgctcc gcatgaagcg acggtttcgg caggaacgaa
    12841 ctgacagatc cgtaccgtat gtctgccgag agtaactcaa cgattcggca gaggtgatcg
    12901 caggacgatt atcgaattcg tccggcagat tgccgcagcc aaagttgacg acattgagtc
    12961 cgccgttcgc tctacgaatg ccccttggta ggaacacata gcg
//
LOCUS       DASRKK010000424         6363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_717633, whole genome shotgun sequence.
ACCESSION   DASRKK010000424 DASRKK010000000
VERSION     DASRKK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717633"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFC15_09915"
     CDS             <1..283
                     /locus_tag="VFC15_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10513.1"
                     /translation="ANVAFTLGVGEISGPLQGGATSGVVLAVLEKQEPSPEELKQNWD
                     RAKETLLDQKRQTLEGLYVQNLRDKLEKEGKIKVNKKEMERMSHLSEGS"
     gene            418..1977
                     /gene="malQ"
                     /locus_tag="VFC15_09920"
     CDS             418..1977
                     /gene="malQ"
                     /locus_tag="VFC15_09920"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZM10514.1"
                     /translation="MFTKRSSGILMHPTSLRSRGGIGDLGPAAYEFVNWLAAAQQTLW
                     QILPFGPAGYGNSPYSCTSAFAGNVLMISLERLVERGYLDRSRLSELPDGEVCVDFEN
                     VRALKLPLLREAARNFLQSAHGVARERYDAFCSANQWWLGDYALFSVLREHFGDNPWN
                     SWPEEIAKRKPKALSQLGTDLKDQLALERFLQFAFFEQWKALRAYSADRGIRIIGDVA
                     IFVSYDSADVWTHPDIFRLHEDLSPEFVAGVPPDAFSETGQRWGNPLYNWEALKSRGY
                     DWWIQRMRWAVETCDIVRLDHFRGYEACWEIPADEPTAINGHWTAGPNDDLFKALRNE
                     LGKLPFIAEDLGYITPEVRVLRRKLDIPGMKVMQFGFGDRGAHIYLPHLFTIDSVVYT
                     GTHDNNTTVGWWDSDASENEKRWALEYLGKVDDGIHWAFIRAAFASVAILAVIPVQDV
                     LGLPADARMNIPSKPGGNWSWRLRDGALTPELANKLATLTTVTDRDACVKVPRSPEDT
                     RRAAMGADFAA"
     gene            2284..2469
                     /locus_tag="VFC15_09925"
     CDS             2284..2469
                     /locus_tag="VFC15_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10515.1"
                     /translation="MGASGELIRMMNYVDDIAATLRRVSGSLHLLADDEKKRLAEYMR
                     KSDPNFVSVLAELEPKA"
     gene            2474..3364
                     /locus_tag="VFC15_09930"
     CDS             2474..3364
                     /locus_tag="VFC15_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZM10516.1"
                     /translation="MAEIHTIAVIGAGIMGRGIAHASALGGYRTILEDILPTTLRKAE
                     TEIRANLDKAIELGKVTPDGAEQAFKRLEYASSVEQAAREADLVIEAVPEEMESKLEI
                     FTLLDKVCRPTTIIASNTSSLSITEISSVTYRAPKCVGMHFFNPVQKMKLLEIVRALE
                     TDDETLAAAVGVGRHMGKEVVVIRESPGFITSRINALIGNEAFYMLQEGVASAEDIDR
                     AIKLGLNHPMGPFEMVDLVGLDTRLHILEYLHKTLGEKFRPAPLMVQYVKAGRLGRKA
                     GRGVYAYPGHEPGRPDSSGD"
     gene            3681..4658
                     /locus_tag="VFC15_09935"
     CDS             3681..4658
                     /locus_tag="VFC15_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10517.1"
                     /translation="MCSFLARKLIAAEIFSLLLLSGMALAQANESDEIRESLSQSIQK
                     DRVSAHLPEAERLLRQAASLDEDTYGRNSRRVAEDLDMLSDVFSEDGKFAEAETTLKA
                     ALSIYDVIDGWRVASNSYYLGRLANLASHQQRFAEAELIYKQLLADQSTEQGFESPAT
                     LRDLAELHHLAKDYPNSEAAYRKVIESKSLEPGSGQILGAIEGLCAVYEGEGKFEQVE
                     VLYRDTIQTNQTILPRGDLVTIAELNDLGLFYERRERWQDAEAYYKRALAQFDGLAPD
                     ASLMDSNLARVIDNYARLLRAEGRFTESEQYENRAKAIRDKLGGNRLTK"
     gene            4911..5267
                     /locus_tag="VFC15_09940"
     CDS             4911..5267
                     /locus_tag="VFC15_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10518.1"
                     /translation="MSEETVIRVRRPRRGAAAIQQLVGEFVDSGLKQSEFCRSRGIAL
                     NTLKRYLKRHGEAGNTGGNFSGLVAVELAAVNPTRRPAGESGLAVVVGSGRRIEVDAG
                     FDAPTLARLVAVLERL"
     gene            5271..5657
                     /gene="tnpB"
                     /locus_tag="VFC15_09945"
     CDS             5271..5657
                     /gene="tnpB"
                     /locus_tag="VFC15_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZM10519.1"
                     /translation="MFGVGAATRIYLAAGTTDMRKGFNGLYGIVRDQLECDPLSGHVF
                     LFVNAQRNRLKVLFWDGSGLWVCAKRLEKGRFHWPESSDGCGKIVLSQEELAMLLGGI
                     DLVQAQRRRWYRKPVTEEPHKLRSPA"
     gene            5713..>6363
                     /locus_tag="VFC15_09950"
     CDS             5713..>6363
                     /locus_tag="VFC15_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HZM10520.1"
                     /translation="MSATSQPTTKSTAGATSSHTDLLEELKKQLAGPLLTAISDQFNS
                     FQKTVISYQNQLEYAELKIQVLEERLRQQRIAKYGPGSEKLSNAQLELLELEPGVSPA
                     EVEAESQRESPTSGKKPRRKHPGRQSLPGDLPRVEKIVGCTPEQCQCGGCGGATTVIG
                     YEESEQLDVEPAKYFVLVTKREKRACKQCEERGVVAAPLPERIIEKSLVSDQVVIDT"
BASE COUNT         1440 a         1654 c         1919 g         1350 t
ORIGIN      
        1 agccaacgtg gcgttcactc tcggtgtggg cgagatcagt ggcccgttac agggcggtgc
       61 gaccagcggc gtggtgttag ctgtgttgga gaagcaggag ccgtcgcccg aagagctaaa
      121 gcaaaactgg gatcgcgcca aggaaactct gctcgatcaa aagcgacaaa cgctcgaggg
      181 actctacgtc cagaacctgc gtgacaaact cgagaaggaa ggcaagatca aggttaacaa
      241 gaaagaaatg gagcgcatga gccatttgag tgagggctcc taggcagctt tcgctttgag
      301 atgtgtggcc agcgaagcaa gtcgctggcc tggtctttct ggcgcccaca agtgagcctg
      361 tgaagatccg gctctgcgtg cttcttactc aagccattta cgatcgaatc gatatccatg
      421 ttcacgaaac gctcatccgg cattctgatg catcccacat ccctgcgctc ccgcggcggg
      481 attggcgatc tcggccctgc cgcctatgag ttcgtgaact ggctggccgc cgcccagcag
      541 actctttggc aaatccttcc gttcggtccg gcaggttacg gcaactcacc gtattcctgc
      601 acttcggcat ttgccgggaa tgtgctgatg atcagccttg agcgcttggt ggagcgtggg
      661 tacctcgatc gttctcggct ttcggaactt cctgacggcg aagtctgcgt ggattttgaa
      721 aatgttcggg cgctcaaact tccccttctg cgcgaggcgg cccgcaactt tctgcaaagc
      781 gcgcacggcg tagcgcgcga gcgttacgat gcattctgct cggcgaatca atggtggctg
      841 ggtgactacg cgctcttcag cgtgttacgt gaacacttcg gcgacaatcc ctggaactca
      901 tggcctgaag aaatcgccaa gcgaaagcca aaagcactat cgcaattagg cacggacctg
      961 aaagaccaac tggcgctgga gcgcttcctg caattcgcat tcttcgagca gtggaaagcg
     1021 ctgcgagcat actccgctga ccgtggtatc cgaatcatcg gcgacgtcgc gatcttcgtc
     1081 agctacgaca gcgccgatgt ctggactcat cccgacatct ttcgcttgca cgaagacctc
     1141 tcgccggagt ttgtggcggg cgttccgccc gatgctttca gcgagaccgg tcagcgctgg
     1201 ggcaatcctc tttacaactg ggaggccctg aagtcgcggg gatatgactg gtggatacag
     1261 cgtatgcggt gggccgtgga aacctgcgac atcgtgcggc tcgatcactt tcgcggatac
     1321 gaagcctgtt gggagatccc ggccgatgag ccaaccgcca tcaacgggca ctggacagcc
     1381 ggtcccaatg acgacctctt caaggctctt cgcaatgaac tcgggaagct gcccttcatc
     1441 gcggaggacc ttggttacat cacgcccgag gtccgcgtac ttcgcaggaa gctcgacatc
     1501 cctggcatga aagtcatgca gtttggtttt ggcgaccgcg gcgcacatat ctatctgcca
     1561 cacttgttca ccatcgattc cgtggtctat accgggaccc acgataacaa caccacagtc
     1621 ggatggtggg acagcgacgc cagcgagaat gagaagcgtt gggcgttgga gtatctaggg
     1681 aaagtagatg acggaattca ttgggccttc attcgtgctg cattcgcctc ggtagcgatt
     1741 cttgcagtca ttcccgtgca ggatgtcttg ggactgcccg ccgacgcccg catgaatatt
     1801 cccagcaagc cgggaggcaa ctggtcatgg cgtctccgtg acggtgcatt aaccccggag
     1861 ttggccaata agttggcgac cctgacgacg gtgaccgacc gggacgcatg cgtgaaggtc
     1921 cctcggtcgc ccgaggatac gcgacgcgca gcaatgggcg cagatttcgc agcttgagtc
     1981 atcgacgctt cgtggcttct ttacttaatt gccactggcg cggagaatga aagtcgggcc
     2041 attcgttcga cttctcgcac ctgcacagcg acttggtgcg ccagggttaa tagagcatat
     2101 ttcgtaactg gttatttgaa atggcacggc ttcacagctt gcggaagaac tcgattctga
     2161 acggttttgt atcggggcac gactgaagtc gtgccgtaaa gcgctttact tttgtcatcg
     2221 cgagggggct tcagcccccg agggatctca tcttcaatgg gttttatatc tgaaaggact
     2281 cctatgggtg ccagcggtga actaatccgc atgatgaatt acgtggacga catagcggcg
     2341 acgctccggc gtgtcagtgg aagcttgcat ctcctggccg acgacgaaaa aaagcgcctc
     2401 gccgagtaca tgcgcaagtc cgatcccaat ttcgtctccg tcctggcgga attggagccc
     2461 aaggcttagt tggatggcag agatccacac cattgccgtc attggcgcgg gaatcatggg
     2521 gcgcggtatc gcccacgcat ctgcgttggg cggataccgc accatcctcg aagacattct
     2581 gccgacaact ttgcggaagg ccgagactga gattcgtgcc aacctcgata aggctattga
     2641 actcggcaag gtgacgccag acggtgccga gcaggcattc aagcgcctag aatatgcctc
     2701 ttctgtggag caggctgccc gcgaggccga ccttgtgatc gaggccgtac ccgaagagat
     2761 ggagtccaaa cttgagatct ttactctctt ggacaaggtc tgccggccta ccaccatcat
     2821 cgcgtccaac acttcgtcgc tcagcatcac cgagatttca tccgtcacct atcgcgcacc
     2881 caagtgcgtt ggcatgcact tcttcaatcc agttcaaaag atgaaattgc tggagatcgt
     2941 gcgcgcgctc gagaccgacg acgaaacgct ggctgctgct gtcggtgttg gccggcacat
     3001 gggcaaggaa gtggtggtga tccgcgagtc tcccggcttc atcaccagcc gcatcaatgc
     3061 gctgatcggc aatgaagcgt tctatatgtt gcaggaaggc gtcgcctccg ccgaagacat
     3121 cgatagggcg atcaagctcg gcctgaatca tcccatgggc ccgtttgaaa tggttgacct
     3181 cgtgggcttg gatacccgcc tgcacattct cgaatacctc cacaaaaccc taggcgaaaa
     3241 attccgtccc gcgccactga tggtgcagta cgtaaaagcc ggacgcctgg gccgcaaagc
     3301 cggccgcggc gtgtacgcct atcccggaca cgaaccgggc cgaccagatt cttccggtga
     3361 ttgagaaata gcttatcgcc atcactcagt gcgaatagct tacgacatgg agatgtcaca
     3421 ttcgctactg tttcagcagc ctgaacgccg taagtgtcgt ccctgtaccg tcttgactgt
     3481 agcgagcaga aaatgcgaat cggtggcagt aaagtcatcc ctgcgaaggc tgggaacgat
     3541 ggggctccaa gccctttggt gcacgaggta ctggcaaatc ctaaccagat actctggatt
     3601 gtgccatttg tgtcagtaat tgagtttctt gctatgctta agcggccaag gtctgctact
     3661 acgctgcaag tgagagccat atgtgttcat ttttggcgcg taaactaata gcagcagaga
     3721 ttttctccct gctgctcctt tccggaatgg ctctcgcgca agcaaatgag tcagatgaaa
     3781 ttcgggaatc tctttctcaa tcaattcaga aggatcgagt atctgcccat ctgccagagg
     3841 cagagcgttt actaaggcag gcagcgagtc tggatgaaga tacgtacggg cggaacagtc
     3901 gaagggtcgc cgaagaccta gacatgctta gcgatgtttt tagcgaagac ggaaagtttg
     3961 ccgaagccga gacgacactt aaggccgcgc tgtccatata tgacgtgatc gacgggtggc
     4021 gagtcgcctc caactcctat tatctgggta gattggccaa tcttgccagt caccagcaaa
     4081 ggttcgcaga agcagagctg atctacaaac aattgctcgc agatcaaagt acggagcaag
     4141 gattcgagag ccctgcgaca ttgcgggatc ttgctgaact tcatcatctt gcgaaggact
     4201 atccgaacag cgaggcggct tataggaagg taatcgaatc gaagtcactt gaacctggaa
     4261 gtggccaaat tctgggcgcg attgaagggc tatgtgctgt ctatgagggg gaggggaagt
     4321 ttgagcaagt agaggttctt taccgagata caatccagac gaatcaaacg atcctgccgc
     4381 gtggcgatct cgtaactatc gcagagttaa atgatctcgg attgttttat gaacgacggg
     4441 aaagatggca ggatgctgag gcatactaca agcgtgccct tgcccagttt gatggcctgg
     4501 cacctgatgc tagcctgatg gattctaatc tggccagagt gattgataac tacgctcgtc
     4561 tcctgcgcgc cgaggggaga tttactgaat cggagcaata tgagaatcgc gcgaaggcga
     4621 tcagagacaa actcggcggc aatcgtttaa caaaatagtg catcagcaca agatcgaccg
     4681 caggcgtctc aggacaaaaa cgggattcga aatctcgatg gggatctaag tgtccacgta
     4741 tttttcttaa tggacactta agcccgcgcg agatgaggcg gaagaacctc ggcgagattc
     4801 caaggcagga gttcctgtag tcggttgaca ggatggcggc gatgcattcc aacgtaagcg
     4861 tgagagcaac gcacctgttt ctgactggct ttatgtgatg aagtcttggg atgagcgaag
     4921 aaacagtcat tcgggtacga cggccacgtc gtggtgcagc agcgatccag cagttggtag
     4981 gagagtttgt tgacagcggt ttgaagcaga gcgaattctg ccgcagtcgc gggatagcgc
     5041 tgaacacact gaagcgttat ctgaagaggc atggcgaagc tgggaacacg ggcggcaatt
     5101 tcagcggtct ggttgcagtg gaattggcgg cagtgaatcc gacacgacgg ccagcggggg
     5161 aaagtggctt ggcggtggtt gtcggatcgg gtcgcagaat cgaagttgac gccggctttg
     5221 atgcgccgac gctggcgcga ctggtagcag tgctggagag gttgtaagcc gtgttcggtg
     5281 tgggggcggc gacgcgcatc tatctggcgg cgggaaccac agacatgcgc aagggcttca
     5341 acgggttgta tgggatagta cgggatcaat tggagtgcga cccgctgagc ggccatgtgt
     5401 ttctgttcgt gaatgcgcag cgcaaccggc tgaaggtttt gttctgggac ggcagcggtc
     5461 tgtgggtgtg cgcgaagcgt ctggagaaag gacgctttca ctggccggag tcgagtgacg
     5521 ggtgcggcaa gatcgtgttg agtcaggagg aattggcgat gctgctgggt gggatcgatc
     5581 tggtgcaagc gcagcgtcgg cgatggtatc gcaagccggt tacggaggaa ccacacaagt
     5641 taaggagccc cgcataaaca ctcggcaaaa ccgttgtact tccacaggcg tgtctgttat
     5701 tccatagcca tcgtgagtgc gacttcccag cctacgacga agtccactgc gggggcaacg
     5761 tcttcccaca ccgatcttct ggaagaactg aaaaaacaac tggctgggcc gctgctgact
     5821 gcgatcagcg atcagttcaa cagttttcag aagaccgtta tctcttatca aaaccagctg
     5881 gagtacgcgg agttgaagat ccaggtgctg gaagagcgcc tgcgtcaaca gcggattgcg
     5941 aagtatggtc cgggcagcga aaagctctcc aacgcgcaac tggagttact agagctggag
     6001 cctggcgtca gcccagccga ggtcgaggcc gagagccaac gcgagtcgcc aacctctggc
     6061 aaaaaaccaa ggcggaagca tcctggccgc cagagtctac cgggggatct tccgcgggtg
     6121 gaaaagatcg tggggtgcac gccggagcag tgccagtgcg gcggctgcgg cggggcgacg
     6181 acggtaatcg gctacgagga gagcgagcag ttggatgtcg agccggcgaa atactttgtg
     6241 ttggtgacca agcgcgagaa gcgcgcctgc aagcagtgcg aggagcgagg cgtggtagcg
     6301 gcgcccctgc cggagcggat catcgagaag tctctggtta gcgaccaggt ggtgatcgat
     6361 acg
//
LOCUS       DASRKK010000425         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1303544, whole genome shotgun sequence.
ACCESSION   DASRKK010000425 DASRKK010000000
VERSION     DASRKK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303544"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            862..2115
                     /locus_tag="VFC15_09955"
     CDS             862..2115
                     /locus_tag="VFC15_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10521.1"
                     /translation="MDHFASLRPTPKAWLLNSQLAPHIDAFAEHLRRGRYAGNTTRQY
                     GAGLAHFAHWMTQCGLPVQVLNEQTVEQFLTNHLPCCDCPMPVQRVHSDLRAALGHLL
                     VVLRVQGVIAKPPQPVGDIADELRRYDEHMDKARGLSATTRSDRVRTVGRLLLHKFAD
                     RPVVIGALEPADVRQFITGQLERCGTVSNAAALTSALRAYFRYRTTCGDRVHGLLGVI
                     SSPARWSLASLPRSLEPAEVERLLKSFTAAVPSHRRSYAIVRCALDMGLRSGEIAKLQ
                     LADIDWRAGTVTLKRTKSLREDILPLPAVTGRALADYVRHERPTTTNPAVFVRCLAPR
                     DQPIGVDAVHRVIRDAYQRIGLTHGRSHALRHTLACRLLDHGSSLKEVADVLRHRSLN
                     TSLIYAKIDTPRLTAVALPWPGSAT"
     gene            2112..3086
                     /locus_tag="VFC15_09960"
     CDS             2112..3086
                     /locus_tag="VFC15_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10522.1"
                     /translation="MSARFDLKAKVDDYLAERHRLGFKLENMQRFLASFTRYVTSVHH
                     QGPLTVDLMANWARQDRAQSHKPGTGARRLKILRPFTRWLRQFEPRTEVPDESVFGPV
                     PGRVTPHIYRETEIVDLLAAARGLNPLGGLRPATYETLFGLIASTGLRVSEALALLDG
                     DVELAKGTLTVRQTKFAKSRLLPLHPSTVEALTRYRQLRSRHMRTATDSPFFISTRGQ
                     LLGQPLGNRQVHRVFIELRDQLGWIDRGAHGGPRIHDMRHGFAVRRVMLWHEQGVDID
                     QSMLALSTYLGHAKISNTYWYLTGVPELMALAGGKFERFAQAPEAGDE"
     gene            3079..4095
                     /locus_tag="VFC15_09965"
     CDS             3079..4095
                     /locus_tag="VFC15_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10523.1"
                     /translation="MNKPKHPASSPSFPSLVQTFFAEHLTQQRAMSPCTVAAYRDTFM
                     LFLNFSQTRLGRSPAAMTMTDITPELILAFLDHLEQERHNAVRSRNARLAALRAFLKF
                     AAHRDVSSLHIIERALGVPMKRFERPMLGFLSLDEMRAVIGPPGSTWLSQRDHLLFGL
                     IYNTGARVSEIIGVRVADVVLDAAACVHLRGKGRKQRSLPLWQSTIKEIRRWLKLNPQ
                     FTPTSPLLPSRNGEAMTRDNVAKRLALAVEKAAKNDPELTKRHISPHSIRHTTAMHML
                     QSGAGTDVIALWLGHESPTTTHHYVEADLRMKERTLARLQEPNSKITRYHAPDSLLEF
                     LKTL"
BASE COUNT          977 a         1344 c         1307 g          946 t
ORIGIN      
        1 ttcttgaatt gcaggagaag ccagtgcaat ggaacgcaat agagtagtcg tcatcctgat
       61 ggccaacacc aaaaacaata gcccaaatca gagaaccgga aaatataccg agaaagacca
      121 cgattgagag caagcgcccc aagatattga tcgcatctag gcgccaaccg ttcaggacta
      181 gatacgggaa ctgcggcagg cgctatcgct gattcctgac gggtcgttta cgtctgaatt
      241 gtttagtcta tctagaacac taaatgcagc gatcactgcg atactgacgg caatgaccaa
      301 atggacacac agcttggcaa cctcaacgta acttacgccg gggccctcaa tttcccggga
      361 aacgaggatc tcggcttcat atttttcgcg aacgattcgt gtcatcaata gatttctgtc
      421 aggtcgtctt aggattaaga gtatagcgga acgcaattga ttcacgattc ggccggtgac
      481 gcggcaattt acttccggaa tggataacgt gtgtaaattc gtcgatgggg gaactgacgt
      541 tcagttgcat tgcattgctg ccgtagggcg ttgcactaac ggccgcacgg gcgaagcttc
      601 acaaatgggc tgtctggcgg gtttcggagt gaagcagtca tcagaacgac gtctcaaatg
      661 agtcgatggc ggactgcttt tggccgttat gcagagctct gcataacggg cattatgtag
      721 agtgcacagt tatgaatagt actcggtcag gcattgctgg caagccttgt aaacactggt
      781 tgagcggggc ttggtgggcc ggaatggtag acataattta agagattgtt agaacaggtg
      841 gctccacaac cggaggccat catggaccat tttgcttcgc ttcgacccac gcccaaggcg
      901 tggcttctca atagtcagct cgcaccccac atcgatgcat ttgctgagca tcttcgacgt
      961 ggtcgttatg ccggcaacac aacgcgccag tacggtgctg gcctcgccca cttcgctcat
     1021 tggatgacgc aatgcggctt gccggtgcaa gtgctgaatg aacagacggt cgagcagttc
     1081 ctgaccaacc acctaccgtg ctgtgactgc ccgatgccag tgcagcgagt tcatagcgat
     1141 ctgcgcgctg cgctcggtca tctgctggtc gtgctgcgcg tacaaggtgt aatcgccaag
     1201 ccgccgcaac ctgtcggcga cattgccgac gaactgcgcc ggtacgacga gcacatggac
     1261 aaggcacgcg gcttgagcgc caccacacga agcgaccgag tacgtacggt aggacgtctg
     1321 ctgctgcaca agttcgcaga ccgtcctgtt gtgatcggcg cgctggagcc cgccgacgtc
     1381 cgccagttca ttaccggaca gcttgaacgt tgtggcacgg tctccaacgc cgccgctctg
     1441 acttcggcgt tgcgcgccta cttccgctac cgaacaacct gcggcgacag agtgcacggt
     1501 ctactcggcg tgatctcttc acccgctcgc tggagcctcg catcactgcc acgcagcctc
     1561 gaacctgctg aggtcgagcg cctgctcaaa tcgttcactg ccgcggtacc gtcgcatcgg
     1621 cgcagctacg cgatcgtgcg ttgcgctctg gatatgggtc tgcgcagcgg ggaaatagcc
     1681 aaactccaac ttgccgacat tgattggcgt gccggtaccg tcacgctcaa gcgcaccaag
     1741 tcgctgcgcg aagacatctt gccactgccg gcggtcaccg gacgcgcact agccgactat
     1801 gttcgccatg aacggccgac gaccaccaac ccagcggtct tcgttcggtg cctggcgccc
     1861 cgcgaccagc cgatcggcgt cgatgctgtg catcgtgtga ttcgcgatgc ctaccaacgc
     1921 atcggtctga cgcacgggcg cagccatgcc ctacgccata cgctggcgtg ccggttgctg
     1981 gatcacggga gctcgcttaa ggaggtggcc gatgtactgc gccatcgatc gctgaacacg
     2041 tcgctcatct acgccaagat tgatacgccg agactgaccg cagtcgcgtt gccttggccg
     2101 gggagcgcga catgagcgcg cgctttgatc tgaaggcaaa ggttgacgat taccttgccg
     2161 agcgtcaccg tctgggcttc aaactggaga atatgcaacg gtttctggca agcttcaccc
     2221 gctacgtaac cagcgtgcat caccaaggtc cgttgaccgt agacctcatg gccaactggg
     2281 cgcggcagga tagagcgcag agccacaagc cgggcaccgg cgccaggcgt ctcaagatcc
     2341 tgaggccatt cacccgttgg ctgcgacagt ttgagccgcg tacagaggtc cccgacgaat
     2401 ccgtcttcgg gccggtgccg gggcgggtga cgccacacat ataccgcgaa accgagatcg
     2461 tcgacctgct ggctgctgcg cgcggtttga acccgctagg cgggctgcga ccagcaacct
     2521 acgagacgct gtttgggctg atcgcctcga cgggcctgcg ggtgtccgaa gcgctcgctc
     2581 tactcgatgg tgatgtcgag ttggcgaagg gcacgctgac cgtacggcag accaagttcg
     2641 ccaaatcacg tttgttgccg ctgcacccga gcacggtgga ggccctgaca cgctaccggc
     2701 aattgcgcag ccgccacatg cgtaccgcca ccgatagccc attcttcatc agcacgaggg
     2761 gccagttgct gggccaaccg ctgggcaatc gccaagtcca tcgggtgttc atcgaactgc
     2821 gggatcagtt gggttggatt gaccgcggcg cccatggcgg cccacgcatt cacgatatgc
     2881 gccacggctt tgccgtccgc cgcgtcatgc tgtggcacga gcaaggcgtc gacatcgatc
     2941 agtcgatgct ggcgctctcg acttacttgg gtcacgcaaa aatatccaac acctactggt
     3001 acctcactgg cgtgcctgag ttgatggctt tggcgggcgg taagttcgag cgatttgccc
     3061 aggcgccgga ggctggtgat gaataagccc aaacatccgg cctcgtcgcc gtccttcccg
     3121 tcgcttgtac agacgttctt tgccgagcac ctgacacaac agcgggcgat gagcccgtgc
     3181 acagtggcgg cgtatcggga tacgttcatg ctgttcctga acttctcgca gacacgtctg
     3241 ggccgatcac ccgcggcgat gacaatgact gacatcacgc ccgaactgat cttggcattc
     3301 ctcgaccacc tggagcaaga gcgccataac gcggtgcgca gccgcaatgc ccgactggct
     3361 gccttgcggg cattcctgaa gttcgcggca catcgcgatg tctcttctct acatatcatc
     3421 gagcgcgcac tcggggtgcc aatgaaacgc tttgagcgcc cgatgctcgg gtttctctcg
     3481 ctcgacgaga tgcgggcagt catcggcccc ccaggcagca cttggcttag ccagcgcgat
     3541 catctgctat ttggactgat ctacaacacc ggggcacggg tatccgagat tatcggcgtg
     3601 cgcgtcgccg acgtggtgct cgatgctgcc gcctgcgtac atttgcgcgg caaggggcgc
     3661 aagcagcgct cgcttccgct gtggcagtcg accatcaagg agatcaggcg ttggttgaaa
     3721 ctgaacccgc aattcacgcc cacttcgccg ctgctgccca gccgcaacgg tgaggcgatg
     3781 acgcgtgaca atgtggcgaa gcgtctggca ctcgcggtgg aaaaggctgc caagaacgac
     3841 ccagagctaa cgaagcggca tatatcaccg cattccattc gtcatacaac cgcgatgcat
     3901 atgctgcagt caggcgctgg caccgatgtg attgcgctct ggttgggtca cgagagccca
     3961 acgacgaccc accactacgt ggaagcagac ttgcgaatga aggaacgcac acttgctcgg
     4021 ctacaggagc ccaactccaa gatcacgcgc tatcacgctc cggactcact attggagttc
     4081 ctgaaaacgc tgtaattatg cagagtctac gagcatggct tcggctacaa aacctagccg
     4141 tagccatgct tgcccaaagg cgctattcat aattgagcac tctacataat ggccgttatg
     4201 cagagctctg cataagttcc attagcggtc cctcgaggaa atctctcaaa gcagccattg
     4261 aatcgttcag agtgtatgct aacgccctag gttttgaggt gctgcttttc acagtttatt
     4321 tagtactgta tgaaatcggt tctcgaacac cgaaaaccct gtcgtgaaga ggcttacaga
     4381 gatattttcc gcgccaagtt agggagcagg cgccggagtt atgcagcaaa actgcgccct
     4441 ataacaaagt tgaactaaac cgctatcgcg gtggttgaga gatcgaccac cgcgattttt
     4501 ttcgtgcctg gcgcaaagaa gcgccgccgt tgctcaaggc ttatgctcgt cgtccgattc
     4561 tcgtgatggg actg
//
LOCUS       DASRKK010000426         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_619475, whole genome shotgun sequence.
ACCESSION   DASRKK010000426 DASRKK010000000
VERSION     DASRKK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619475"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFC15_09970"
     CDS             <1..486
                     /locus_tag="VFC15_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZM10524.1"
                     /translation="FDHMLLRVDLEEPWLVDVGFGDSFVDPIVFRAGGADQVNGHRYL
                     VSPLGEEWQLLREDDKGAVPLYVFRDVPRQLGEYEEMSALHQTSPESGFTKRWICSKA
                     APDGRITVANRRLIITAADRREESVLSTDDEVRRCLRDHFGIEFDDTADLSRLDSFPN
                     G"
     gene            complement(585..1121)
                     /locus_tag="VFC15_09975"
     CDS             complement(585..1121)
                     /locus_tag="VFC15_09975"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZM10525.1"
                     /translation="MRKSRLGMFAMLVIAMCTTACVAQEKKPLSPPGQASVKFDDGKT
                     VTIQYSRPSMRGRKIFGGLVPYGQVWRTGANAATSLKTDVDLTIGGTTVPAGSYTLYT
                     LPRMNSWKLIINKQTGQWGTEYSEGQDLARVDMKVAQLPSGMEMFTISLGKTSATSAM
                     LKLDWENTIASVEVKEKK"
     gene            1205..1471
                     /locus_tag="VFC15_09980"
     CDS             1205..1471
                     /locus_tag="VFC15_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10526.1"
                     /translation="MSILDERREELDQYEFMMGVPRGRLAVALDVLTDALTMVGQHGV
                     YCQSARQPGMPAMDIQIVMKGITDAKELISSVMEEIRKQKETEA"
     gene            complement(1697..1996)
                     /locus_tag="VFC15_09985"
     CDS             complement(1697..1996)
                     /locus_tag="VFC15_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZM10527.1"
                     /translation="MANGKLGIDQPARRKDLKIATKRRGTLGKSRSVRTYTAVIHRED
                     HWYVAACPEVGVVSQGKSIDEAIANLKEATELYLEEFPPPSSERSLITTFDVTHA"
     gene            complement(2149..>3160)
                     /locus_tag="VFC15_09990"
     CDS             complement(2149..>3160)
                     /locus_tag="VFC15_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM10528.1"
                     /translation="IIAFNILNVGRFKLGASCVGGARTSLQHSIAYAKERKAFGKSIA
                     EFGLIREKLAQMAAGIYASESMVYRTVGMMDTALSAIEHNPETVRKAIEEYAVECSII
                     KVSASEMLDMVVDETVQVYGGYGFVEEYPAEQAYRDSRVNRIFEGTNEINRLIITGWL
                     LKRAMSGQLALLPAIKKTMDEVMAGPSTDELEGTLAAERKLVGNGKKLAMLVAGAASQ
                     KYMQKLVDHQEVMGAIADMVIETFAMDSCLLRARKYVEANGEGKGALVVAMTQVYLEG
                     AFAKLEAAAKKVIAAVAEGDMLRTQMAIVRRLAKHDPVNVIALQESIASRVLETGKYV
                     TA"
BASE COUNT          619 a          946 c          893 g          702 t
ORIGIN      
        1 ttcgatcaca tgctgctacg ggttgacttg gaagagccat ggttggtgga tgtcggattc
       61 ggagactcgt ttgtcgatcc gatcgtgttt cgcgccggtg gcgccgacca ggtgaatggg
      121 catcgctatc tggtgtcgcc acttggagag gagtggcagt tgctgcgtga agatgacaaa
      181 ggcgcggtac cgctttacgt ttttcgcgac gtgccgcgcc agctcggcga gtacgaggaa
      241 atgtcggcgc tccatcaaac gtcaccagag tccggcttca ccaaacgctg gatctgtagc
      301 aaggccgcac cggatggccg catcaccgtg gccaaccggc ggctgatcat cacggccgca
      361 gaccgtcgcg aggagagcgt cctcagcacc gatgacgaag tgcgccgatg cctgcgcgat
      421 catttcggaa tcgagttcga cgacacggcg gacttgtcgc gactggacag ctttccgaat
      481 ggctaggccc gcagcgggcg gaaaaagatt caccacagag acacagagaa atagaatcag
      541 gatcggcccc ggatctccat tcggctccgg cttcgtcccg ggcgctactt cttctctttc
      601 acctccacgc tggcgatggt gttttcccaa tccagcttca acatggccga ggtcgcgctc
      661 gttttgccca gagagatggt gaacatctcc atgcccgatg gcagttgagc taccttcatg
      721 tccacccgcg ccaggtcctg gccttcgctg tattccgtac cccattggcc ggtctgcttg
      781 ttgatgatca gcttccagct attcatgcgg ggcagggtgt aaagggtata gctgcccgca
      841 gggacggtag tgccaccgat ggtcaggtca acgtcggtct tgaggctggt tgccgcgttc
      901 gctccggtgc gccagacttg gccatacggg acaagtccgc cgaatatctt ccgtccgcgc
      961 atggatggac gactgtactg gatggtgact gtctttccat catcgaactt gaccgacgct
     1021 tggcccggag ggctgagcgg ttttttctcc tgcgccacgc aggctgtcgt gcacatagcg
     1081 atgacgagca tggcgaacat tcctagacgg cttttccgca ttgcactctc ctgttgattg
     1141 caaattctca tttcctgttg ccgctctatt attcacgccg gggcggaacg atacaattca
     1201 acccatgagc attctggacg aacggcgtga agaactggac caatacgagt tcatgatggg
     1261 tgtgccgcgg ggacgtctgg cggtggcgct cgatgtcttg acggatgcgc tgaccatggt
     1321 gggccagcac ggcgtctatt gccagagcgc gcgccagccc ggcatgccgg ccatggatat
     1381 ccagatcgtg atgaagggca ttaccgacgc aaaggagctg atctcgtcag tgatggaaga
     1441 gatcagaaaa cagaaagaaa ccgaggcgta acaaactcag atcatcttcg tgaacgtttc
     1501 cggatcaacc tgcgcctgac tcaaaatgcc acggagtgtg cctgtcgcaa gctcagagtg
     1561 caggggcacg acgcagccta ttgcaccggt atcagttaca cgttttaaca caacgtgact
     1621 gccacgctgt cgcacccgca caaacccaag cttcccaaga gcgcggatcg ctgcagctcc
     1681 tgacattcgg ggcagcttag gcatgcgtca cgtcgaatgt tgtaatcagt gatcgctcag
     1741 acgacggcgg cgggaattct tccaagtaaa gttctgtcgc ttccttcagg ttggcaattg
     1801 cttcgtcaat actcttgcct tggctgacga cgccgacttc gggacatgcg gctacgtacc
     1861 agtgatcttc ccggtggatg acagctgtgt aggttcgcac agatctggat ttgcctagtg
     1921 tgccgcgacg ctttgtcgcg atcttgagat cttttcggcg tgccggctga tcgataccaa
     1981 gcttcccgtt tgccatgctg ttcctctctg agcgcagaat tcagtcccta cgattggcgg
     2041 ctgcgtccaa aaactccctt accggacata aatatacagc agaaacgtag agcggagact
     2101 atgcgctccg ccctgcgatg tgtaacccgc tctgctgccg atttcgtttt acgccgtcac
     2161 gtacttcccg gtctccagca cgcggctggc gatggactcc tgcaaggcga tcacgttgac
     2221 tgggtcgtgt ttcgctagcc ggcgcacaat tgccatctgg gtgcgcagca tgtcgccttc
     2281 agcgacggca gcgatcactt tcttggccgc agcctccagc ttcgcaaatg cgccctccag
     2341 atagacctgc gtcatggcga caaccagcgc gcccttgcct tcgccattgg cctcgacata
     2401 cttgcgggcg cgcagcaggc aggaatccat ggcaaaggtc tcgatcacca tgtcggcgat
     2461 ggcgcccatg acctcctgat gatcgaccag cttctgcatg tacttctgcg aggctgctcc
     2521 tgccaccagc atggccagtt tcttgccgtt ccccaccagc ttacgctcgg ctgcgagcgt
     2581 gccttcgagt tcgtccgtcg acgggcccgc catcacctcg tccatggtct tcttgatggc
     2641 cggcagcaac gcaagctgtc cgctcatggc ccgtttcagc agccatccgg tgataatgag
     2701 ccgattaatt tcgttggtgc cttcgaagat gcggttgacg cgcgagtcgc gatacgcctg
     2761 ctccgccgga tactcctcga cgaagccata gccaccgtaa acctgcaccg tttcgtccac
     2821 caccatgtcg agcatctcgg atgccgacac tttgatgatg gaacattcga ccgcatactc
     2881 ctcaatcgcc ttgcgcacgg tctcgggatt gtgctcaatc gcggagagtg cggtgtccat
     2941 catgccgacg gtgcgataca ccatcgattc gctagcgtag atcccggccg ccatctgcgc
     3001 cagcttctca cgaatcaagc cgaactccgc gatgctcttg ccgaaggcct tgcgctcctt
     3061 ggcataggcg atggaatgct gcaacgaagt gcgagcgccc ccgacgcagc ttgcgcccag
     3121 cttgaagcgg ccaacattca ggatgttgaa cgcgatgata
//
LOCUS       DASRKK010000427         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_228636, whole genome shotgun sequence.
ACCESSION   DASRKK010000427 DASRKK010000000
VERSION     DASRKK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228636"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFC15_09995"
     CDS             <1..211
                     /locus_tag="VFC15_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10529.1"
                     /translation="VMKLFRRERVLTQICGNNFMVLKSAPPLVAKEQHIEEFVESLGR
                     VVAEVHNSTAFWSEALGLARRAMNV"
     gene            278..955
                     /locus_tag="VFC15_10000"
     CDS             278..955
                     /locus_tag="VFC15_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM10530.1"
                     /translation="MRLLVVEDDSAIREFLNRGLTEAGYRVDAAPDAKSALALAGEEV
                     YDGFIVDLGLPDMDGLDVIGRCRAQGHTAPVLILSARRSVDERVRGLEQGGDDYLTKP
                     FALAELLARVRNLLRHNTPTQSEPTCLRVADLELDLIRRDARRGQESLQLTAQEFSLL
                     EYLVRNAGRVVTRTMILDRVWRMRIDPATNVVDVQIYRLRNKVDGEGKRPLIHTIRGV
                     GYVLKEA"
     gene            936..2372
                     /locus_tag="VFC15_10005"
     CDS             936..2372
                     /locus_tag="VFC15_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM10531.1"
                     /translation="MFLRKRKSQGRSAAWRIAIWPTVAFALGSAVAFAIMYLLIARDI
                     RERSDAWLSGEAEVLADVSENTPRDALYDRLVQEVAELASREVPDTSDAYGKHQSSVF
                     FLKTEPRQEPIWVGPTPKELFISAVTSMPLVLGAPANVRVQGWKKAFRVVYHAQKDGG
                     GLYLGFADLGGAQMLDRLTERCLLVWGATVALGFLITWLAAYRTLIRVERISETVSRI
                     GSEDLSSRLPEGPHSDEISLLSRTFNRMLDRVQSSVHQMRILTDSIAHDLKSPVTSVR
                     GSLEVALSDGHDGRWQECVAEAIDGLDRLSQLLNTTLDLAEASAGALQTRKEPVELSA
                     LVQQLVDLYLPAMAERGHEVVTHLQPVVIEADSSLLNRTIANLLDNEIAHLPPGCRIE
                     ISVSAKQGEAELTVQDNGPGFPPELRSRAFERFVKGPHSAGHGLGLAFVDAVVRAHGG
                     QVEIADHDAGGAVIMLTLGEAVSERVSR"
     gene            complement(2398..2973)
                     /locus_tag="VFC15_10010"
     CDS             complement(2398..2973)
                     /locus_tag="VFC15_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZM10532.1"
                     /translation="MLFAWLLLLPSMAAAQQIAVNLDPAQTKIQWTLADVLHTVHGTF
                     KMRSGSVTFDPKSGAASGEIIVDATSGESGNHSRDQKMHKEVLESQRYPEIIFSPQRV
                     MGKLADQGTSNLQVQGVFHIHGANHDVTLSLPVEKSGDTVRASTSFVVPYQDWGMKNP
                     STFLLKVENKVSISISSVGRIRIDRAASPAH"
     gene            complement(3055..4173)
                     /locus_tag="VFC15_10015"
     CDS             complement(3055..4173)
                     /locus_tag="VFC15_10015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM10533.1"
                     /translation="MYKSIRHAKVLVAGGGPAGLAAAIAARQAGFAVTVVDGAHPPID
                     KACGEGIMPDGLAALRQLGVRIGPEQGAPFRGIRFLNDDAQVEADFPHGAGYGIRRTV
                     LHQLLVDRATEAGISLHWGARITGLSAAGVVIGGQHVGYRWLICADGHNSLLRRSAGL
                     DVFRSARRRFGFRRHYRVAPWSNFVEVHWSNCGQMYVTPVSDSEICVALITRSRGLAW
                     EEALPHFPLLRSHLKDAEAGDRHLGALSMTRRLAAVHLGNIALVGEASGSVDAVTGEG
                     LSMAFRQAVALAEAMRAGNLPQYEMAHRRIARLPRMMSTLMLAMDKHPGFRRRVFRAL
                     TSEPDYFRRLLALHTGAISPLNFGLRETVSLGWHLLAA"
     gene            complement(4179..5231)
                     /locus_tag="VFC15_10020"
     CDS             complement(4179..5231)
                     /locus_tag="VFC15_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZM10534.1"
                     /translation="MTRIAGVASAFPRQYYRQQEIANVLKRHWDDGLGNPKVLDRLLT
                     RVGVDGRYLALPIEAYDDLTTWGKSNNAWIEVAEELAEQSICRALTRAGFCEADLDAI
                     FFVSVTGVASPSIDARLMNRMNLRPDIKRNPIFGLGCVAGAAGLARAADYVRAFPEQC
                     AVLVSVELCSLTWQRKDLSVANLISSGLFGDGSAAVIVAGSERSAAGPQVLGSRSSFY
                     RGTEEVMGWNISEQGFRIVLSPEVPEMVRQHLARDVDRFLADYGLDRGDIDTWIMHTG
                     GPKVLEATAQSLDLPDGALDVSWQTLRRVGNLSSASVLLVLEEFMNRRRPEAGSYSIL
                     AAMGPGFCSELVLLRW"
BASE COUNT         1172 a         1635 c         1726 g         1116 t
ORIGIN      
        1 ggtgatgaaa ctcttccgcc gcgagagggt tcttacgcag atatgtggga acaacttcat
       61 ggtgctgaag tcggcccccc cgttggtcgc gaaagaacag cacatcgagg agttcgtgga
      121 atcgctcgga cgagtcgtgg cggaagttca taactctacc gcattctgga gcgaggctct
      181 gggacttgcg cgccgtgcga tgaacgtgtg atgcagtaga aaactggtct ctgcgctatc
      241 cttaagagtt tccaaagccg atggcgagag gagagatgtg cgactgttag tcgtggagga
      301 tgattcggcg attcgagagt tcttgaatcg cggcctgaca gaagctggct atcgtgtgga
      361 tgctgcaccc gacgcgaaat cggcgttagc cctcgcgggc gaagaagttt acgacggctt
      421 cattgtcgac ctcggattgc ccgacatgga tggcctggat gtgatcgggc gctgtcgcgc
      481 ccagggccac accgctccag tgctgatttt gtcagcgcgc cgctccgtcg atgagcgggt
      541 ccggggactg gaacagggcg gtgatgacta tctgaccaag cccttcgcgc tggctgaact
      601 gctggcccga gttcgcaatc tgctgcggca caatacgcca actcagtcgg aaccgacttg
      661 tttgcgggtc gccgatctgg agttggacct gatccggcgc gatgcccgca gagggcaaga
      721 gtcccttcaa ctgacagcgc aggagttctc tttactggag tacctggttc gtaatgctgg
      781 acgggtggta acccgcacta tgattttgga ccgcgtgtgg cgcatgcgca tcgatccggc
      841 gaccaatgtg gtggatgtgc agatctaccg tttgcgcaac aaagtagacg gcgaaggcaa
      901 gcgcccacta atccatacca tccgcggtgt tggctatgtt cttaaggaag cgtaaatcgc
      961 aggggcggtc agcggcctgg cgcatcgcca tctggccgac cgtggctttc gcattgggaa
     1021 gcgcggtggc cttcgcgatc atgtatctac tgattgcgag agatattcgc gagcgcagcg
     1081 acgcctggct gagcggagag gccgaagtgc tggccgatgt ttcggagaat accccgcgtg
     1141 atgcgctgta tgaccgcttg gtgcaggagg tggcagagct tgcttcccgg gaagtgccgg
     1201 atacgagcga tgcctatggc aagcaccaat cttctgtttt ctttctgaag acggaaccgc
     1261 ggcaggaacc catctgggtg gggccgactc caaaggaact gtttatctcg gcggtgacga
     1321 gcatgccgct ggtcttggga gccccagcca atgttcgcgt gcagggatgg aaaaaggctt
     1381 tccgggttgt gtaccatgcc cagaaggacg gcggcgggct gtatctggga tttgccgacc
     1441 tcggcggcgc gcagatgctg gatcgcctga cggagcgctg ccttttggta tggggtgcaa
     1501 ccgttgccct gggatttctg atcacctggt tggcagcata ccgcaccctg atcagagtgg
     1561 aacgcatcag cgaaacggta tcgcgtatcg gcagcgaaga cctaagcagc cggttgccgg
     1621 aaggcccaca ttcggatgag atctccctct tgtcgcgcac cttcaaccgc atgttggacc
     1681 gggtgcagtc atcggtacac cagatgcgca ttctcaccga ctcgatagcg cacgatctga
     1741 agagcccggt cacctccgtc cgtgggagcc tggaggtcgc gctttctgac gggcacgatg
     1801 gacgatggca ggagtgtgtc gccgaagcca tcgacggtct cgaccggctt tcgcaattgt
     1861 tgaacacgac gctggatctc gccgaagcca gcgccggagc gttgcaaacc cgcaaggaac
     1921 cggtcgagct ctcggcactg gtgcaacagt tggtggacct ctacctgcct gccatggcgg
     1981 agcgtggaca tgaagtcgta acgcatctgc agccggtggt gattgaggca gattcgtcgc
     2041 tgttgaaccg gaccatcgcc aacctgctgg ataatgaaat agcccatctt ccgcccggct
     2101 gccggatcga gatctcagta agcgcgaagc agggcgaagc cgaacttacg gtgcaagaca
     2161 acggtccggg atttcccccc gagcttcgca gccgcgcctt cgagcgtttt gtgaagggac
     2221 cgcattccgc tggacacggt ctgggactgg catttgttga cgcggtggtc agggctcacg
     2281 gaggacaagt tgagatcgcg gaccacgatg ccggcggagc ggtaattatg ctcacgttgg
     2341 gcgaagccgt aagtgagagg gtcagccgct aaagccgcgt tgccaataca tcaaactcta
     2401 gtgtgccggg gatgctgcgc ggtcgatccg gatgcgacct acgctggaga tactgatgct
     2461 taccttgttc tcgactttca acagaaaggt gcttgggttc ttcatgcccc aatcttgata
     2521 gggcacaaca aaactggtgc tcgccctgac cgtgtcaccg ctcttttcaa ccggcaagga
     2581 cagtgtgacg tcatggttgg cgccatggat atgaaacacg ccttgcacct gtaggttcga
     2641 tgtcccttgg tcggcgagct ttcccattac acgttgggga gaaaaaatga tctccggata
     2701 acgctggctc tccagtacct ctttgtgcat tttctggtcg cgagagtgat tgccgctctc
     2761 gccgctggtg gcatccacaa tgatttcgcc gctggcggcg ccggatttcg ggtcgaaggt
     2821 aacgctgcca ctcctcatct tgaaagtgcc atggacggtg tgcagcacat ctgccagcgt
     2881 ccactggatt ttggtttggg cgggatcgag attaaccgcg atctgctgtg ccgccgccat
     2941 cgacggcaat agcaacaacc aggcgaacag cacgatacaa cccgtacgaa acgttcggcc
     3001 tgaatgaatt ggagacccga gcctcaaatt gatgctcctc tgattcgccc cgatttatgc
     3061 agccagcaaa tgccatccca gcgacacggt ctctcgcaag ccaaagttca agggcgagat
     3121 cgctccggtg tgaagcgcca acaaccggcg gaagtagtcg ggctccgatg tcagtgcacg
     3181 aaacactcga cgacgaaaac cgggatgctt gtccatggcc agcataagcg tggacatcat
     3241 gcgcggaaga cgtgcaatgc ggcggtgcgc catttcatat tgcgggagat tgccggcccg
     3301 catcgcctcg gcgagagcga cggcctgacg gaatgccatc gacagccctt cgcccgtcac
     3361 cgcgtcaacg gagcccgatg cttctcccac gagggcgatg ttgccaaggt gaacggccgc
     3421 gaggcgacga gtcattgaca aagctcccaa gtgacggtcg ccggcctccg cgtctttcag
     3481 gtgagaccgc aataagggaa agtgaggaag cgcctcctcc catgcaagcc cacggctgcg
     3541 cgtaatcaag gccacgcaga tctcgctgtc cgacaccgga gtgacgtaca tctgaccgca
     3601 attgctccag tgtacctcca caaaatttga ccagggcgcc actctgtagt gcctgcggaa
     3661 tccgaatcgc cgtcgtgcgg agcggaagac gtcgaggccg gcacttctgc ggagcaaaga
     3721 gttgtgtccg tcagcgcaga tcaaccaacg atagcccacg tgctgcccgc cgatgacaac
     3781 tcctgcggcg gatagaccgg tgatgcgtgc gccccagtgc aaggaaatgc ctgcctccgt
     3841 cgcccggtcg accagcagtt gatgcaaaac cgtgcgccga attccatagc cagccccgtg
     3901 agggaaatcc gcctccacct gtgcgtcgtc attaaggaaa cgaatgccgc gaaatggcgc
     3961 tccctgctcg gggcctatac ggacgccgag ttggcgaagt gccgccaagc catccggcat
     4021 gatgccctcg ccgcacgctt tgtcgatggg agggtgcgcg ccgtcaacca cggtgacggc
     4081 aaaaccagct tgccgggctg caatcgcagc cgccagtcca gcggggcctc cgccggcaac
     4141 cagcaccttg gcatgcctaa tggatttgta catggcctct accagcgcag cagcacgagt
     4201 tccgagcaaa agcccgggcc catggctgca agaatgctgt aactgcctgc ctccggacgg
     4261 cgccggttca tgaactcttc cagcaccagc agcacagacg cagatgaaag gttgccgacg
     4321 cgccgcagag tctgccatga gacgtcgagc gcaccatcgg gaaggtccaa ggactgcgca
     4381 gtagcctcca gcaccttggg gcctccggtg tgcatgatcc acgtgtcgat atcgccgcga
     4441 tcgagcccgt agtcggcgag aaagcgatca acatctcgcg ccaggtgctg gcgcaccatc
     4501 tccggaacct ccggcgaaag tacgatccta aaaccctgtt ccgagatgtt ccaacccatc
     4561 acctcctcgg tgccgcgata gaacgacgac ctgctgccga gcacctgagg gccggccgca
     4621 ctgcgctcgc tcccggcaac gatcaccgcg gcggaaccgt cgccgaaaag cccagacgaa
     4681 atgagattgg cgaccgagag gtctttcctt tgccacgtga gcgagcagag ttccaccgaa
     4741 accagcacag cgcactgctc gggaaatgcg cggacatagt ctgccgcacg agccagaccg
     4801 gccgcccctg cgacacatcc gaggccgaag attggattgc gcttgatgtc cgggcgcagg
     4861 ttcatacggt tcatcaggcg cgcgtcaatc gaggggctgg ccacaccggt gaccgagacg
     4921 aaaaaaatcg cgtccaggtc agcttcgcaa aagccggcgc gcgtaagcgc acgacagatg
     4981 gactgctccg ccagctcttc ggcgacctcg atccacgcgt tgttcgactt tccccacgtg
     5041 gttaggtcgt cataggcttc gatgggcaaa gccaaatagc gtccatcgac accgacccgg
     5101 gtcagcagac gatcgaggac cttagggttt cccaacccgt cgtcccagtg ccgtttgagg
     5161 acgtttgcta tttcttgctg gcgatagtac tggcgtggaa atgcactggc tacgcctgcg
     5221 attcgcgtca tcttcatcat ccgcaccgcg cgccctgcgg ctcgtgtacg ggcgcggcgc
     5281 gtgccttctt tcttggtcca atccgtttgt aggaggaaaa taccccgttg ctgccagact
     5341 agcggctaga catgaagagc tcatttcctc cgcatgacaa gtcattcatc tgctagtcga
     5401 tgggaatgct ttgactccgt cgcacggtca tgttctggat gggagtgtca gctattcgga
     5461 agttggggga gctacttgca ttggtcattt gatggaaaat agccttctcg gtgcgccggg
     5521 ctaaaccggc atgaagtagg gaatgaaaga gtcctacgag gaaggagtag cgaaccactc
     5581 ggccccgagt tttgcgtaat gcaccgtgag gtgtactgcg aagcgtaaac aggggatagg
     5641 tgggctggg
//
LOCUS       DASRKK010000428         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1304467, whole genome shotgun sequence.
ACCESSION   DASRKK010000428 DASRKK010000000
VERSION     DASRKK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304467"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="murF"
                     /locus_tag="VFC15_10025"
     CDS             <1..1431
                     /gene="murF"
                     /locus_tag="VFC15_10025"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZM10535.1"
                     /translation="AEHWLLNRATDAVCNVMKLSLSRAAEFMQATGEFAPAAVASGYS
                     IDSRTLLPGELFFAVRGDRLDGHDFVEAALARGAIGAVIAMDQRTRFAQKNQILVVDD
                     PLLALQRLAAAVRRLWGKPLIAVTGSAGKTTTKEAIAHVLATRHRVLKSQGNLNNHFG
                     MPLQLLKLEPEHEIAVVEMGMSHAGEITGLAKLAQPDCGVVTMVAPVHLEFFESIAAI
                     ARAKYELIESLPAGGIAVLNVDDEYVLQFGRDFHGKVVTFGLQKPADVSARKIESHGP
                     LGSVFEIVADGEHVPAALPLLGEHSIYNALAAVAVGLQYGVPLRMAAGSLATLAPGDK
                     RGEILNYAGATIINDCYNSNPKALDSMVRSLAQIPARRRIVVAGEMLELGPAAEVMHR
                     DCGRHMATYGIDRLLGVRGLAKSMVEAAAGAGMNAEFVNTPQEAADWLRREVKTGDVV
                     LLKASRGVRLERALEAWVGKTPEPTH"
     gene            1673..>2737
                     /gene="mraY"
                     /locus_tag="VFC15_10030"
     CDS             1673..>2737
                     /gene="mraY"
                     /locus_tag="VFC15_10030"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZM10536.1"
                     /translation="MLYWLLYQRGFLVFHVGPFRIFRYLTFRTVFASLTALFIGLIIG
                     PAVVRRLREFQIGQYIREDGPKDHLKKAGTPTMGGLLIVVGMIVPTLLWADLSNRFVW
                     IAVLATLSFGAIGFADDYLKVVHRRNLGLTARAKLLYQFLAAFVIGAILSALETHGLY
                     STRLIVPFLKHFRPDLVIGRLHHGYLSPLAFLPFLAFVAIIIVGSSNAVNLTDGLDGL
                     AIGCTVIAAGALTVLTYVSGHAVFADYLELSKMPQVSEVTIFCGSMVGSAIGFLWYNA
                     HPAEIFMGDVGSLALGGAIGTVAVIIKQELLLPFIGGIFVIEALSVILQVGSYKLRRK
                     RIFKMAPIHHHFELMGWSESK"
BASE COUNT          509 a          776 c          859 g          593 t
ORIGIN      
        1 gcggagcact ggctactcaa ccgcgcaacc gacgcagtct gcaacgtcat gaagctaagc
       61 ctatcgcgcg ctgccgaatt catgcaggcc actggcgagt tcgccccagc cgcggttgcc
      121 agcggctatt ccatcgactc gcgcaccttg ctgccgggcg agctgttctt cgcggttcgc
      181 ggcgatcggc tggacgggca tgatttcgtc gaggcggcgc tcgccagagg cgcgatcggc
      241 gctgtcattg ccatggacca gcgcacgcgg ttcgcacaga agaatcagat attagtggtg
      301 gacgatccgc ttctggcatt gcaacgactc gctgctgccg tccgccgatt gtggggaaag
      361 cccctcatcg ccgtgactgg gtcggcgggc aagacgacaa ccaaggaagc catcgcgcat
      421 gtattggcta caagacatcg ggtactgaag tcgcagggca acctgaacaa ccatttcggc
      481 atgccgctgc aattgctcaa actggagccg gagcatgaga tcgcggtagt cgagatggga
      541 atgtcgcacg ccggcgagat cactggactc gcgaagctgg cacagccgga ttgcggcgta
      601 gtgaccatgg tcgctccggt gcacttggag ttttttgagt cgatcgcggc aatcgcgcgt
      661 gccaagtacg aactgataga gtcgcttcct gccggcggta tcgctgtgct gaatgtggac
      721 gatgagtacg tcttgcagtt cgggcgagac ttccatggga aagtcgttac cttcggacta
      781 cagaagccgg ctgacgtgtc ggcgcggaag attgaatcgc acggcccgtt gggctcggtg
      841 ttcgaaattg tggcggatgg cgagcatgtc ccggcggccc tgccgttgtt gggcgagcac
      901 agtatctata acgcgctggc ggccgtcgcc gtcggattgc agtatggggt gccgctgcgg
      961 atggccgccg ggtcgcttgc gactctggcg cccggagaca agcggggaga aattctgaac
     1021 tacgcgggcg cgactattat taacgattgc tacaattcca atccaaaggc cctcgacagc
     1081 atggtccggt cactggccca gattcccgcg cggcgccgga ttgttgtcgc cggcgagatg
     1141 ctggaactcg gtcccgcggc tgaggtcatg caccgcgatt gcggacggca catggcgacc
     1201 tatggaatcg acaggttgct gggggtccga ggactggcga agtcgatggt ggaagccgca
     1261 gccggggcgg ggatgaacgc ggagttcgtc aacacgccgc aagaggccgc cgattggctg
     1321 cggcgcgaag tcaaaaccgg cgatgtagtt ctgctcaagg cgtcacgcgg agtgcgtctg
     1381 gagcgtgcgc tcgaggcttg ggttggtaaa acgccggagc ctacacatta aggcagtttc
     1441 acacttggcg tactagggtg gagcaccgct tcagcggtgc aggtaagatt cggaaatgag
     1501 acggctttag ccgctgaggt gacggagaca ttacctcagg cgctaaagcg tgatccagga
     1561 actgacggca gttgtgcacg cctgaaggcg tgctccaccc atcacctaaa cgccagaggt
     1621 ggaagtgctc taggattgca gctcctgtat agctttcaga acggggaaga atttgctcta
     1681 ttggttgctt taccagagag gttttctcgt atttcacgtc ggcccgttcc gaatcttccg
     1741 ctatttgact ttccgtaccg tgttcgccag cctgaccgcg ctgttcattg gccttattat
     1801 tggcccggcg gtggtccgcc gcctgcgcga attccagatt ggccagtaca tccgcgaaga
     1861 cgggcccaag gaccacctga agaaagccgg cacccctacc atgggcggct tgctcattgt
     1921 ggtgggcatg atcgtcccaa cgttgttgtg ggcagatctc agtaaccgct tcgtgtggat
     1981 tgccgtattg gcgacgctgt cgttcggggc gatcggtttc gccgacgact atctgaaagt
     2041 ggtgcaccgc cgcaatctcg gtctaaccgc gcgagccaaa ctgctttacc agttcttagc
     2101 ggcttttgtg ataggcgcga tcttgagtgc cctggaaacc catgggctgt actccactcg
     2161 tctgattgtg cccttcctca agcatttccg gccagacctg gtgatcggcc gattacacca
     2221 tggctaccta tcgccactgg cgtttctgcc gttcctcgcg ttcgtggcca tcatcattgt
     2281 gggctccagc aatgccgtga atctcaccga cggtctcgat ggattggcga ttggctgcac
     2341 tgttattgct gcgggagcgc tcaccgtgct cacctacgtc agcggccacg ccgtatttgc
     2401 cgattacctc gaactttcca agatgcccca ggtctcggag gtgaccatct tctgcggctc
     2461 gatggtcggc tccgccatcg gtttcctctg gtataacgca catcccgcgg agatcttcat
     2521 gggcgatgtg ggatcgctgg cgctgggcgg cgcgatcggg accgtggccg tcatcatcaa
     2581 gcaggaactg ctgctaccgt tcatcggcgg catcttcgtg attgaggcgc tctcggtcat
     2641 tcttcaggtg ggttcctaca agctgcgcag gaagcgcatt ttcaagatgg cgcccatcca
     2701 ccaccacttt gagctgatgg gctggagcga gtcgaag
//
LOCUS       DASRKK010000429         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_815988, whole genome shotgun sequence.
ACCESSION   DASRKK010000429 DASRKK010000000
VERSION     DASRKK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815988"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFC15_10035"
     CDS             <1..699
                     /locus_tag="VFC15_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10537.1"
                     /translation="QSRSVASTTVTALRSFLGYLYANGLIKQQLTAAVPAVARWRLAA
                     LPKTLQPREVQALLAACDRRTRMGRRDFAVLTMLVRLGLRASELAGLRLDDIDWRAGS
                     ILIRGKGNQIEPLPCPQDVGDAVVAYLRRGRPASAADRSVFVRIRAPHRALSGIGVSG
                     IVVAAARRAGLGEIRAHRLRHTVATQLLRAGSSLPEIGQLLRHRHISTTAVYAKVDRD
                     ALRSIARPWPGGAA"
     gene            696..1622
                     /locus_tag="VFC15_10040"
     CDS             696..1622
                     /locus_tag="VFC15_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10538.1"
                     /translation="MSPLRQALFDYLAVRRSLGFKLGRAAGLLAQFITYAEERGEQHL
                     RISTMLAWATLPVNTHRNWLAKRLSIVRGFASHLHTIDPATEVPPADLLPWQRCRATP
                     YLYSDDEVAALVTAATSLQTPYRVLTYQTLIGLLAVTGMRVGEALALDRDDIDMTHGV
                     LTVRLTKFGKSRELPVLASTVEALGRYLGHRDRPRAAASIAAAFISTSGTRLVYANVL
                     WTFQRLLRRAGITRRSAACRPRLHDLRHRFAVQTMLDAYRQDENAEARIAILSTYLGH
                     VNPKATYWYLSAAPELMQLAADRLERHLGGVS"
     gene            1619..2608
                     /locus_tag="VFC15_10045"
     CDS             1619..2608
                     /locus_tag="VFC15_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10539.1"
                     /translation="MSALAPTLQAFFTDRLIRQRQASDNTLAAYRDTLRLLVRFAAER
                     RKVEPVELEIGDLDSALISAFLDHRERECGNSVRTRNTRLTAIRSLFRYAALRHPEHA
                     EVIQRVLAIPPKRFARRLVSFLTDPEVDALLSAPDRTTWVGRRDQTLFATAVQTGLRV
                     SELIGLRITDVHLGVGPHVSCLGKGRKQRITPLTKPVVALLRTWLCERREQVSDLVFT
                     TRTGKMLSRDALEHRIAECVAKASNNCPSLREKKISLHVLRHTAAMRLLHAGIDLSVI
                     ALWLGHEQMETTQIYLHADLALKEKALARTTPVNTSPGRYRPPDTLLAFLEAL"
BASE COUNT          531 a          822 c          766 g          572 t
ORIGIN      
        1 caatcgcgaa gcgtcgcaag cacgacggtg actgcattgc ggtcattcct tggctacctt
       61 tacgccaacg gcttgatcaa acagcagttg acagcggctg ttcctgcggt agctcgatgg
      121 cgcctggctg cgctcccgaa gaccctgcaa cctcgcgagg ttcaggcgct tttggcagcg
      181 tgtgatcggc gcacacgaat gggacgtcgg gattttgccg tcctgacgat gctcgttcgc
      241 ctcggactgc gcgcgagtga actcgcggga cttcgcctcg atgatatcga ttggcgtgct
      301 ggctcgatcc ttattcgagg gaagggcaat cagattgagc cgctaccgtg tccgcaagat
      361 gtcggcgacg cagtggtcgc ctatctgcgc cgaggacggc ccgcaagcgc ggcggatcgt
      421 agtgtattcg tccgcatcag agcacctcac cgcgctctta gcggcatcgg ggtgagcggc
      481 attgttgtcg cggcggcccg tcgcgcaggc ctcggcgaga tccgcgcaca tcgactgcgc
      541 cacacagtgg ctacccaact ccttcgtgcc gggtcctcat tgcctgagat tggccagctg
      601 ctgcgccacc gccatatttc aactaccgcg gtctatgcca aggtcgatcg agacgcacta
      661 cgaagcatcg cgcgcccgtg gccaggaggt gcagcatgag cccgctacga caagctctct
      721 tcgattacct cgctgtccgc cgttcgctcg ggttcaagtt gggcagggcc gcgggactgc
      781 tcgctcaatt catcacttac gccgaggaac ggggagagca acatttgcgt atctccacga
      841 tgctcgcatg ggcgaccctg ccggttaaca ctcatcgaaa ctggttggca aaaaggcttt
      901 caatcgtgcg tggcttcgct tctcacctgc acacgatcga tcctgcgacc gaagtgccgc
      961 cagccgatct gttgccctgg caacgatgtc gcgctacgcc ttacctctac tccgacgacg
     1021 aggtggccgc gctggttact gctgcgacga gcttacaaac gccctatcgg gtgctgacgt
     1081 atcagacctt gatcgggttg ctggcggtca ccgggatgcg cgtcggcgag gcccttgcgc
     1141 tcgaccgtga cgacatagat atgacccacg gtgtactgac cgttcggctg accaagttcg
     1201 gcaaatcgcg cgaattaccg gtacttgcga gtacggtcga ggctctgggt cgctatctcg
     1261 ggcatcgcga ccgtcctcgt gcagcggcaa gtatcgccgc agctttcatt tcgacctcag
     1321 gaactcggtt ggtgtacgcc aatgtgctgt ggacatttca acggctccta cgccgcgccg
     1381 gaatcacgcg gcgatcggca gcgtgccgtc ccaggctcca tgatcttcgt catcggtttg
     1441 cagtccagac aatgcttgac gcctaccgcc aggacgaaaa tgcagaagca cggattgcga
     1501 ttctatcgac atacctaggc cacgtgaacc cgaaggccac ctattggtat ttgtctgctg
     1561 cacccgaact aatgcaacta gccgctgatc gcctcgagcg ccacctcgga ggtgtctcat
     1621 gagtgcgctc gccccgacgt tgcaggcgtt tttcaccgac cggctcattc gccagcggca
     1681 agcaagcgac aacacgttgg cggcgtaccg cgacaccttg agattgctcg tgcgtttcgc
     1741 agccgaacgc cgaaaagtag aaccggtgga gctggaaatt ggtgatctgg attccgcact
     1801 gatcagcgcc ttccttgatc atcgtgaacg cgagtgtgga aatagcgtac gcacacgcaa
     1861 cacccggctt acagcgattc ggtcactgtt tcgctacgca gcccttcgtc atccggagca
     1921 tgcggaagtc atccagcgcg tacttgcgat tccaccgaag cgtttcgcgc gtcgccttgt
     1981 gagctttctc acagatccgg aagtcgacgc acttctttcg gcgccagacc gaacgacctg
     2041 ggttggccga cgcgaccaga cgctgttcgc gacggcagtc cagacaggac ttcgtgtatc
     2101 ggaactgatt gggttgcgga tcactgacgt tcatctaggc gttggtccgc acgtcagttg
     2161 cctcggaaaa ggacgaaaac aacggatcac gccgctcacc aagccagtgg tggcgttgct
     2221 ccggacctgg ctctgtgaac gccgtgaaca agttagcgat ttggtcttta ctacgcgtac
     2281 tggaaagatg ctcagccgtg acgcactcga acatcgaatt gcagaatgcg ttgcaaaggc
     2341 cagtaataac tgtccgtcct tacgagaaaa gaaaatcagc cttcatgtgc tgcggcacac
     2401 ggcagcaatg agacttttac atgccgggat cgatctctcg gtcatcgccc tctggctcgg
     2461 tcacgagcag atggaaacta cgcagatcta ccttcatgcc gacctcgctc tcaaagaaaa
     2521 agcactcgct aggactacgc ccgtaaatac cagtcccggc cgttaccggc cacccgatac
     2581 gctccttgcg tttcttgagg cgctctgaat atgccgacat cgttctgtcg cggaccacct
     2641 gtttagggca tgtgtgcctg ccggatcggc atattctgaa agtcggcatt c
//
LOCUS       DASRKK010000430         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_131416, whole genome shotgun sequence.
ACCESSION   DASRKK010000430 DASRKK010000000
VERSION     DASRKK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131416"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFC15_10050"
     CDS             <1..433
                     /locus_tag="VFC15_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10540.1"
                     /translation="GFKAVSRRHEASNPVAEVVHQARATAASVHATYEVSSMREPQAA
                     PEKSAPARSEAVKPSELESARAAERPRTIPHVPDNLDDIDSEIVRHAEPMDEAQVQAR
                     AAAAAGRDEPPTIETVSSSVNINYDADDLEIPAFLRKRGEG"
     gene            complement(668..1489)
                     /locus_tag="VFC15_10055"
     CDS             complement(668..1489)
                     /locus_tag="VFC15_10055"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZM10541.1"
                     /translation="MSRCLRLLLACLVFSLLSLPMVAQAPPSADTYVTRANPATNYGS
                     GPILAVQDGTTTYLQFDLSALPEGASIRKATLRLYVNSVLAAGSFDVYQVNDNWSENT
                     LNYSNAPPLGASATAVNPINVSAASVNHFVMVDITPLVRDWLTGATENNGIALALAES
                     PTANGGSFSFDSKENFQTGHEPELEIVLSASAAPICSESLGQGATSAATGCSSESLRT
                     CCQCNNSYNGFNYCVNDLATRSQCVNFCAGKGGIHDYLNNFKCNVSNPGPNFVGP"
     gene            1936..>2687
                     /locus_tag="VFC15_10060"
     CDS             1936..>2687
                     /locus_tag="VFC15_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM10542.1"
                     /translation="MISVSNVSMRYGAKVLFDEVSTAFTPGKRYGLTGPNGAGKSTFM
                     NLLTGELEPQKGTVVRPRKLGVLRQDQFAFDAYRVLDTVIMGNKRLWAAMEERDRIYE
                     KHEITNDDGMKLGELEGIVGEEDGYSAETDAALLLQGLDIPDEMHQRSMSELQGGQKV
                     RVLLAQALFGHPQALLLDEPTNHLDLDSIHWLQSFLRNYDGVVIVISHDRHFLNSVCT
                     HIADIDYETIITYTGSYDEMVMAKTQIRSQIEG"
BASE COUNT          606 a          754 c          767 g          560 t
ORIGIN      
        1 cggcttcaag gctgtttccc gccggcacga ggcatcgaat ccggtggctg aggtggtgca
       61 ccaggcgcgt gcgacggcag ccagtgtcca cgcgacctac gaagtgtcga gcatgcgcga
      121 gccgcaagcg gcgcctgaaa agtctgcgcc ggcccgctcc gaagcggtca agccgtcaga
      181 acttgaatcc gcgcgggccg cggaacgacc gcggacgatt ccgcacgtcc ccgacaacct
      241 cgacgatatc gattccgaga tcgtgcgcca tgccgagccg atggacgagg cacaagtcca
      301 agcgcgggct gccgccgctg ccggtcgaga cgagccgccc acgattgaaa ccgtgagtag
      361 cagcgtcaac atcaactatg acgcggatga cctggagatt ccggccttcc tcaggaagcg
      421 cggagaaggc tgagcgacgt cagggtgtcg gtcgttcaag aagagccccc acccaaactt
      481 cgcttgatgg gcgacccggc ctgccgagta cagtggctcc caggttagcg tccaaaaaac
      541 ggacgcgaac ctggggcacc aggcacccgg ctcgttctca gagaaccatg tgttctccag
      601 cgaaggacga aatattttct cctggacatt ccccctaata gaacccgact tcgcacctgg
      661 aaactgccta tggtcccaca aagtttggcc ccggattact tacattgcat ttaaagttgt
      721 tgaggtagtc gtgtatgcct cctttgcctg cgcagaaatt cacgcactgg ctgcgtgtag
      781 cgaggtcatt cacacagtag ttaaacccgt tgtagctgtt gttacattgg cagcaagtgc
      841 gtaaactttc gctgctgcat cctgtagctg ccgatgtggc tccttgtccc agagattcag
      901 agcatatagg ggccgcggag gcgctgagta cgatttccag ctccggttcg tgcccggtct
      961 gaaaattctc tttactatcg aaagagaaac taccaccgtt agcagtggga ctttccgcca
     1021 aagccagtgc aataccgttg ttctcagtag ccccggttag ccaatcgcgt actaaaggcg
     1081 taatgtccac cattacgaag tggttgacgc ttgcggcgga gacattgatc ggattcacgg
     1141 ccgttgcgga tgcacctaag ggaggcgcat tgctatagtt cagcgtgttc tccgaccagt
     1201 tgtcgttaac ctgatacacg tcgaacgagc ccgcggcgag gacgctgttt acatagagcc
     1261 gcaaagtcgc ttttcttatg ctggcgccct cgggcaaagc agacagatca aactgcaaat
     1321 atgtagttgt gccgtcctgc accgccagga tggggcccga accataattt gtggcggggt
     1381 tggcgcgagt tacgtaggta tcggcactcg gcggcgcttg ggccaccatt gggagcgaaa
     1441 gtagtgaaaa gacgaggcaa gcgagtaaga gacgcaagca acgagacatg gcgcactcct
     1501 taaacgaaga gttggtgtta cgtggccggg gcaatttgcc cgataagtgc attagatggg
     1561 gccgaaacta ctatgaaaac tgcgccttac tatgggttcg gaaccgcagc ctatttcggg
     1621 ggatcaacag ttcactgttg tttcgcgcac aatgcaccaa gtccattcca aggtcaagcc
     1681 cacttaagtg actatttagc gagaaagcga caactatttc gttgatagga tcactactgt
     1741 atagcttgct gtctctgcgc cgtcagctcc gcgaagcgct catcctcgcg caagctgaag
     1801 ccattccgat ggccgtgtgc gcccaaaccg caagccacct ctgtttcgct gcgctctcgc
     1861 gcgttcatga tattcttata tctacggctg cgaccctctt tgtaggcaat ttcccgcagt
     1921 tcccttaaga aaatcatgat ctcagtcagc aatgtatcca tgcgctacgg cgctaaagtt
     1981 ctgttcgacg aagtgtccac cgcgtttaca cccggcaaac gctacggact caccggtccc
     2041 aacggggcgg ggaagtccac cttcatgaat ctattgaccg gcgaattaga gccgcagaag
     2101 ggcaccgtgg tgcgtccgcg gaagcttggt gtgttgcgcc aggaccagtt cgcctttgac
     2161 gcctatcgcg tgctcgacac cgtcattatg ggcaacaagc ggctgtgggc ggcgatggaa
     2221 gagcgtgacc gcatctacga gaagcacgag atcaccaacg acgacggaat gaagctgggc
     2281 gaactggagg gcatcgtcgg cgaagaggat ggatactccg ccgagacgga cgccgcgcta
     2341 ctgctgcaag gcctggatat tcccgacgaa atgcaccagc gttcgatgtc ggagttgcag
     2401 ggcgggcaaa aggtccgtgt gctattggcc caggcgctct ttgggcatcc gcaggcgctg
     2461 ttgctggatg agccgacgaa ccacctcgac ttggactcga tccactggct gcaaagcttt
     2521 ttgaggaact atgacggagt tgtgatcgtc atctcgcatg atcgccattt tctgaacagc
     2581 gtttgcaccc acatcgccga tatcgattac gaaaccatca tcacctacac cggcagctac
     2641 gatgagatgg tgatggccaa gacgcagatt cggtcgcaga tcgaagg
//
LOCUS       DASRKK010000431         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1403003, whole genome shotgun sequence.
ACCESSION   DASRKK010000431 DASRKK010000000
VERSION     DASRKK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403003"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..787)
                     /locus_tag="VFC15_10065"
     CDS             complement(440..787)
                     /locus_tag="VFC15_10065"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM10543.1"
                     /translation="MRHPQLYRFHQWQANLRILEVTQIKSIPYVPLSHPFAERLIGTI
                     RRECLDHMLFWTTVDLENKLLDFRTYFNHHRTHTSREERTPDSPASRPVANLRSFRWQ
                     PHCRALYQTPVAA"
     gene            complement(1009..1551)
                     /locus_tag="VFC15_10070"
     CDS             complement(1009..1551)
                     /locus_tag="VFC15_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM10544.1"
                     /translation="MKQTASMDFFTVPTATFRVLSVFLVLSHDRRRIVHFNVTEHSTE
                     DWTAQQIREAFPWDEAPRYLIRDRDAIFGKDMVATTKAMGIEEVVIAPRSPWQNPYVE
                     RLIGSIRRECLDHMIVWNERSLCRILQSYFHYCEGSRTHLALAKDAPVPRAVDQPENG
                     RIEAISQVGGLHHRYQRRAA"
     gene            complement(1681..2112)
                     /locus_tag="VFC15_10075"
     CDS             complement(1681..2112)
                     /locus_tag="VFC15_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10545.1"
                     /translation="MDTENLVRTDNAYVTIVCEIIIAFKMSPVFIALVALVGSTFQTR
                     AAMQAEIMALRHQLAVLQRSAPRRLRLKQSDRVLWMLLSWLWPNWRHELRILKPATAV
                     RWHRRGFARYWTWKSRRRPGRPRVALAIRDLIQQMCQANFL"
     gene            2347..2853
                     /locus_tag="VFC15_10080"
     CDS             2347..2853
                     /locus_tag="VFC15_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10546.1"
                     /translation="MLYKILWGCALTAFAVAATSAQTKSSISGTCAKADVEQSVPAGD
                     QPGHLFMVAQGKCTTKGEVGGQTSKSGAYSEHRDVTANRIKAAGVYVETYDSGDKIIY
                     NYQMSMGLKDGALQAGKGTYQVTGGTGKFKGIKGTGTCAYTGGANGTNDYSCTGDYTL
                     AGGAAAPK"
     gene            complement(3835..4173)
                     /locus_tag="VFC15_10085"
     CDS             complement(3835..4173)
                     /locus_tag="VFC15_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10547.1"
                     /translation="MLNRSAIVVKPRQPFLDWLHTADPTSHRLTLRELSREPTVYLIP
                     GCDTEEDVRAVLRDLSEEIFIEQLAGWLTDESTWPRDRSFDVFCCWFDFQHHSVLVDL
                     CDEPLIDESD"
     gene            complement(4197..>4823)
                     /locus_tag="VFC15_10090"
     CDS             complement(4197..>4823)
                     /locus_tag="VFC15_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM10548.1"
                     /translation="VSETTVAKYMVRHRHPQSQTWRTFLSNHVKELVSADFFVVPTAT
                     FRLLFVFVILSHDRRRPVHFAVTSHPTAEWTAQQLLQAFPWDTAPRYLLRDRDGNYGK
                     VFRETALGLGIKEVLCAPRSPWQNAYAERLIGSIRRECLDHVIVLHETGLRRVLRSYF
                     EYYAHSRTHLSLEKDTPIPRAAQPPEFGKVVELREVGGLHHRYERRAA"
BASE COUNT         1103 a         1351 c         1363 g         1006 t
ORIGIN      
        1 gttaaatact cctcacccac ccaacaatcg ccgccactac tatgaccccg gggcgcctat
       61 ttcgcggtgg atgcgcggtg atcgatggtg cgacggacgt caccctgccg gaaagctccg
      121 ccacactgcg cagagtgctt cttgtcgaca ccaggagccc tcgacactcc tactgctcgg
      181 ctccggactc ctcgtcacgg gggtgttatt cgacgacgaa ccgtaagcta tgaagttgtg
      241 tctggcgaat tgttacgggg agccgatttt gccggtaatc gcgctgcgaa agtgggctga
      301 gtctcccttt ggggacgcac ccaatcgcag tgaaacgacc ggacctgcga agcacgctcc
      361 gatggagaaa caccgaatga tctcttggaa gttttgccga ggttgaccga atatcgcagc
      421 gagcgtgagt ctttgaaaat caggcagcca ccggtgtctg atagagggcc cgacagtggg
      481 gttgccatcg aaacgagcgg agattggcga ctggtcgtga cgctggcgaa tcgggcgttc
      541 gctcttcccg tgaggtatgc gtgcgatggt ggttgaagta ggtcctgaag tcgagcagtt
      601 tgttttcgag atccaccgtc gtccagaaca acatgtgatc taaacactcg cgtcgaatgg
      661 tgccgattag tctttccgcg aacggatggg acaggggaac gtaagggatg gacttgattt
      721 gtgtcacctc cagaatccgc agattggctt gccactggtg gaaccgatag agctgtggat
      781 gccgcaaact ccatccgggt gttcggcggt tggactttgc agattcagat gggcccattt
      841 caggtatgag acatcgcaaa ttggagatca cgccgcggta gcaaggcgca caaggacgag
      901 aagcaccccg gacttgaata gagtgcgctt gctatcgcaa attccaggca tgctcagaaa
      961 acagaagaag ggaattgttc ctggacacag ccaacgacat tcacgaggct aagcggcgcg
     1021 ccgctgatat cgatgatgga gtcctccaac ctgagaaata gcctcgatac gcccgttctc
     1081 tggctggtcc accgccctgg gcaccggcgc atctttggcc aatgctaaat gcgtacgcga
     1141 gccctcacaa taatgaaaat aactttgcag aatccgacac aacgatctct cgttccatac
     1201 gatcatgtga tccaggcact cccggcgaat cgatccgatt agccgctcca catacggatt
     1261 ttgccaaggt gaccgcggcg cgatcaccac ttcttctatg cccatcgcct tcgtggttgc
     1321 gaccatgtct tttccgaaga tcgcatcgcg atcgcggatc aggtaccgcg gtgcttcgtc
     1381 ccacggaaac gcttctcgaa tctgctgtgc ggtccagtct tcggttgagt gttccgtaac
     1441 gttgaagtgc acgatgcgcc ggcggtcgtg cgataacacg aggaagacgg acagcacccg
     1501 gaaggttgcc gttggcaccg tgaagaagtc catcgaggcc gtctgcttca tgtggttctt
     1561 caggaaggtc cgccaggtct gcgaaggcgg cttccggtgt cggcgcagat acttgcccac
     1621 cgttgacggg gcgacctcga tgcccagctt gagtatctcc ccgtgaatgc ggggtgctcc
     1681 tcacaagaag ttggcttggc acatttgttg aatcaagtcg cgaatcgcta aggccacacg
     1741 cggtcttccc ggacgacggc gggacttcca cgtccagtaa cgggcgaacc ccctccgatg
     1801 ccagcggacg gccgtggccg gcttcaagat gcgcagctca tgtcgccagt tcggccagag
     1861 ccacgacaac agcatccaca gcactcgatc tgactgcttc agacgcagac ggcgcggtgc
     1921 gctgcgttgg agaacagcca gctggtgacg cagcgccata atctcggcct gcatagcagc
     1981 gcgggtctga aacgtcgagc cgaccagggc aacaagtgcg atgaaaacag gactcatctt
     2041 gaaagcgatg ataatctcac aaacgatcgt aacgtatgcg ttatcagtcc ggaccaagtt
     2101 ttcggtatcc acaggcgtag tttcaccact attttcacca ctttcgccaa agtttcacca
     2161 ccttttgact ccagcagtac cagcaagatg gggcttcctg cagttgtttg aggcgctgct
     2221 cgggtgccct acccgcaagt gctgccgaca aaaatcgctt tatagtatct ggggaccagt
     2281 gagacggtgt actatggcgc agctttcgaa gccaaagaaa gaatctgttc gaactggaga
     2341 gaaaacatgc tctacaagat tctgtggggg tgtgcgttaa ccgcttttgc ggttgcagcg
     2401 acctcggcgc aaacgaaaag ttccatttcc ggcacgtgtg ccaaggccga cgtcgagcaa
     2461 agtgtccctg ccggtgacca gccgggccac ctgttcatgg tcgcgcaagg aaaatgcacc
     2521 accaagggag aagtcggagg ccagacgagc aagtcgggcg cgtattccga gcaccgggac
     2581 gttacggcca accgcatcaa ggccgcgggc gtctacgtgg aaacctatga cagcggcgac
     2641 aagattatct acaactatca aatgtctatg ggcctgaaag atggcgcatt gcaggcaggt
     2701 aaaggcacat atcaggtgac aggcggcacc ggcaagttta aaggcatcaa agggacgggc
     2761 acctgcgcgt acaccggcgg cgccaatggc acaaatgact actcctgcac cggggactac
     2821 acactggcag gaggcgctgc ggctccgaag taagggacgg aaaaacagat cgcaactgaa
     2881 aggtgagggc tgtcttgttg tcagcgcttc actttaattc gtgattccga ccaggaactg
     2941 acatcccgat ctcgccgtaa catttccagc gtggcggaca ctccgtcgag ttcgagttcg
     3001 cttttgagtt tgccgagatc aaagaacgcg tcgtcgtaat cggcatcgat tgtgatccat
     3061 ttgcaacgct gcaattgagg cggctaaccg attgaaaaca aatattgtat tgccgaacgc
     3121 caagcaaccg cttcaccaat tcgatctctt gtcctcagcc acttcagggg gaatcggcta
     3181 tcccaccgct acggattcct tccagttgat gttgcaccct aggctcggac gctgctgctg
     3241 agagacaggc ttgccggcga gcaacgcatc cattgcgcta cgcagatcgc agccagtcac
     3301 cgggacacag tttccaggtc tgctgtcatc aaattgtcct cggtagacca actgcagctt
     3361 ttcgtcgaag agaaagaaat cgggagtgca ggcagcatgg tacgccctgt ggttaccgaa
     3421 aactccgtcc gatctgaaaa cacaggggtt ccgatgattt gtaagatcat ggtggctacc
     3481 acgatgagtt ctctgttcgt cgcccttctt gctctgctag cctccagttt tcggactcgt
     3541 gcgatcctgc aggcggagat cctcgcggcg ccaggagcaa cccgaataac tgtgcctgct
     3601 cgttgaggag ctcagtttca atcggcctgt caccgtgaaa taggcttgat ttctgggttg
     3661 attttgaacc cacgattcca acccacgatt cgcatgtcta tcacgacttc tcacgtactt
     3721 aggcggcctg tccctaacga aaactctgtg cggccgatag cggatgtcac tgcccatgtc
     3781 gttcgcccaa aagagatcgc ttcgttatcg ggcggaagtg caggcgccgc gacgtcaatc
     3841 agattcgtcg atcaacggtt catcgcagag atctaccagc accgaatggt gttggaaatc
     3901 aaaccaacag cagaagacat cgaaactgcg gtcacgaggc cacgtgcttt catcggtgag
     3961 ccaacccgcc aattgctcga tgaaaatctc ctcgctaagg tcccgcaaaa cagcgcggac
     4021 gtcctcttcc gtgtcacatc ccgggatcag gtagaccgtc ggctcccgag ataattcacg
     4081 tagcgtgagt ctgtggctgg ttggatcggc cgtgtgcagc cagtccagaa aaggttgcct
     4141 tggcttgacc acgatggcgg agcgattcag cataaatcac cggaagtccg ctactctcac
     4201 gcggcacggc gttcataacg gtggtgcagc ccaccgacct cgcgcaactc taccaccttc
     4261 ccgaactcag gtggctgagc agcgcgagga atcggtgtgt ccttttcgag tgataggtga
     4321 gttcgcgagt gggcgtaata ctcaaagtac gatctcaata cacgcctgag acctgtctcg
     4381 tgcaagacga tcacgtgatc cagacattcc cggcgtatcg acccgattag ccgctccgca
     4441 taggcattct gccagggtga gcgcggagca caaagcactt ccttgatccc aagcccaagg
     4501 gcggtttcac ggaatacctt gccgtaattg ccgtcacgat cgcgcaacaa ataacgcgga
     4561 gccgtgtccc aggggaaggc ctgcaagagc tgctgggctg tccattccgc ggtcggatgg
     4621 ctggtcaccg cgaagtgcac tggtcgtcga cggtcatgag aaaggatgac gaacacaaac
     4681 agcaagcgaa acgtagcggt cggaacgacg aagaaatcgg cggagaccag ctccttgacg
     4741 tgattgctca agaaggtacg ccacgtctgg gattgcgggt gccggtgccg caccatgtac
     4801 ttggcgaccg ttgtctccga gac
//
LOCUS       DASRKK010000432         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1012058, whole genome shotgun sequence.
ACCESSION   DASRKK010000432 DASRKK010000000
VERSION     DASRKK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012058"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(530..1111)
                     /locus_tag="VFC15_10095"
     CDS             complement(530..1111)
                     /locus_tag="VFC15_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10549.1"
                     /translation="MSKVATKVSLALIMASMAVLLAACSIHVDDKDKKNEKVDIQTPF
                     ANLKVDTGDKAADNGIPVYPGAHLRPEENGDNHSANINIGAAGFGLKVIAAEYETDDS
                     PEKVKSFYLAKLKSFGDVLVCSGHSGGSDVHVGSHGDDQDQKLTCKDTHGDGWELKTG
                     TSDNEHLVSIEPRGTGTRFGTVLVRTHGKEGTL"
     gene            complement(1108..>2081)
                     /locus_tag="VFC15_10100"
     CDS             complement(1108..>2081)
                     /locus_tag="VFC15_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZM10550.1"
                     /translation="ITDALDMGGLRQSFPQEGSEGAGRAAVESVKAGSDVLLLPFDLD
                     GSYNGLLRAVRRGEISEARIDQSVLKILLAKASVGLHKAKLVDIHAVNQIVAKPANLE
                     AAQEVADRAVTLVRDNHRALPLKAAGKGTKPPPNPYQPVAETRNSTVLLIFTDDSRSD
                     SGRTLDHELRARIPDARVYYIDPRDAAGMTQPVLTAVQESQAVIAAVYEFPVAGRVVR
                     AQDGAAVNAIAVQESSATLLQQVLRSAAGKTIVVAVGSPYIAAQLPEVQTYICTFSSG
                     HGSELSAVKAMFGEIPMTGHLPVTIPNIAGRGAGLTGAAQDSSGGPQ"
BASE COUNT          400 a          647 c          593 g          441 t
ORIGIN      
        1 tgggtggaag cggcggtggt ggtggcaaac tgtcttcagg aggaaggact cgaatctggg
       61 gagcgccgca gtgcggacaa aacgtagtgc cctcgtcaat cgaggcttgg caacgatagc
      121 agggatgttc cactagccga aagttacagc agatgcgccc cgcacgcaac cggagcgctt
      181 caaagcctca gccagcggta tccacaatcg gcaaggttcc ggcaggcagc tttcttgtgt
      241 cgtccctgaa gggactcggg gcaattctcc actctaccca gcactccgcc tgcggctccg
      301 tgctgggcta aactgagccg cgcctagggc gctggatttt cggcaggtgg acgctgctgg
      361 caaatctgaa agtgtgggag cgggcaatcg caagcccgaa gggcgtcaca atcgtagccc
      421 agcacgtaag tcctgggtaa gcgtcgcaaa cgaaccgagt ccttttaggg acggcacctc
      481 atccgaaaat gtctgcctag ttgaactcga tgcctaattt cgccgcagct tacagcgttc
      541 cttctttacc gtgcgtgcgg accagcaccg tgccaaagcg cgttcccgta ccacgcggtt
      601 cgatcgagac caggtgttcg ttgtcgctgg tgccggtctt gagttcccat ccatcgccgt
      661 gcgtgtcctt acaggtcagc ttctggtctt gatcatcgcc atgcgatcct acgtgaacat
      721 ccgagccacc gctgtggccg ctgcatacca gcacatcgcc gaagctcttg agtttggcga
      781 gataaaaaga tttgaccttt tccggcgagt cgtcggtctc gtactccgct gcgatcacct
      841 tcaggccaaa tcccgcggcg ccgatattga tgttggcgga atggttgtcc ccgttttctt
      901 cgggacgcaa gtgtgcgccc gggtaaaccg gaatgccgtt gtccgccgcc ttatcgccgg
      961 tatccacctt caggttggca aaaggagtct gaatatcgac cttctcgttc ttcttgtcct
     1021 tatcgtccac atgaatcgag caggcagcca gcagcaccgc catgctcgcc attataagag
     1081 ctaatgaaac cttcgtagcg accttactca ttgtggtcct ccgctggaat cttgtgccgc
     1141 gccggtcagt cccgcaccac ggccggcgat gttcggaatg gttacaggca agtgaccggt
     1201 catcgggatt tcgccaaaca ttgccttcac cgcactcaac tcagaaccgt gaccgctgga
     1261 gaaggtgcaa atgtaggttt gcacctcggg caattgtgcg gcgatgtagg gactgcccac
     1321 ggccacaacg atcgtcttcc ccgccgcgct tcgcaaaacc tgctgtagca atgtcgcgga
     1381 cgactcctgc acggctatcg cgttgacggc tgcgccgtcc tgcgctcgca ccaccctgcc
     1441 cgccacgggg aactcgtaca ctgcggcgat caccgcttgc gactcctgca ccgcagtcag
     1501 caccggctgg gtcatccctg ccgcgtcgcg gggatcgatg taatagaccc gggcgtcagg
     1561 aattctcgcc ctgagctcat ggtccagcgt tctccccgaa tcggagcggc tgtcgtccgt
     1621 aaaaatcaac aagacagtgc tattgcgcgt ctccgctact ggctgatacg gattaggcgg
     1681 aggcttggtt ccctttccgg ctgcttttaa gggaagcgcc cgatgattgt cccggaccag
     1741 cgtaaccgct cggtcagcga cctcttgcgc agcctccagg tttgcgggtt tggccacgat
     1801 ctgattcacg gcatggatat cgaccagttt ggccttgtgc agcccaaccg aggccttggc
     1861 gagcaggatc ttcaacaccg attggtcgat gcgcgcttcg gaaatctccc cacggcggac
     1921 ggcccgcagc aatccgttgt atgagccgtc gagatcgaaa ggcagcagca gcacatcgct
     1981 acccgccttc acggattcga ccgcggcccg gcccgcacct tcggaccctt cctgcggaaa
     2041 cgattgccgc agcccaccca tgtcgagtgc gtcggtgatg a
//
LOCUS       DASRKK010000433         6749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_816168, whole genome shotgun sequence.
ACCESSION   DASRKK010000433 DASRKK010000000
VERSION     DASRKK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816168"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            749..1318
                     /locus_tag="VFC15_10105"
     CDS             749..1318
                     /locus_tag="VFC15_10105"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZM10551.1"
                     /translation="MIGFWTRLSYAVRCFFAILLHAEIPQDITRELIKPARDIASLGR
                     TESPSTAVSTKELDTSGTESVDRAVQMLSLLQRDGRLIDFLAEDVAAYPDAQLGAAVR
                     SIHTSCRQVLERYVKLDPVIDSEEDQPVTVPAGFDPAAIKLIGNVTGEPPIRGVLRHR
                     GWRVKEVTLPSLPQGAGRAIVAPAEVEIP"
     gene            1385..3217
                     /locus_tag="VFC15_10110"
     CDS             1385..3217
                     /locus_tag="VFC15_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM10552.1"
                     /translation="MDSQFIIGIDLGTTNCAMARSSISAAEAGSGIELENIPQLVNPN
                     EVADRTLLPSFLYIPGEMDFPKGSLKLPWDAEPKFVIGELARKRGAENPVRLVASAKS
                     WLSYTAVNRTAPILPWQAPEEVSKLSPLEASSQYLRYLRTVWDSSHPEQSLSEQDVLL
                     TVPASFDEEARELTLRAAEQAGLQHVTLLEEPQAAFYAWLETQGDSWRKRIKVGDLIL
                     VCDVGGGTTDFSLITVSEESGDLTLKRVAVGDHILLGGDNMDLALARLLQQRLETEGH
                     RVDTHHLLGLWHQSRLAKEFLFENPQKQKHDVTLLGKGRKLVGGTIKTELLREDVDRV
                     LLEGFFPKVGSDEMPLRQRRIGFQELGLPYAADAAVTKHLARFLAQQVQNSPEAANIR
                     RGRSGLACPTHVLFNGGVMKAAALRSRLVEVLNLWLAGEGFEALGRAHILDAPDLDHA
                     VARGAAYYGRARRGQGVRIRSGASRTYYIGIESAMPAVPGMEAPLKALCVVPFGMEEG
                     TEAQVPDREFGLVVGEPAEFRFLSSTIRKQDHVGDLLENWGSEIEELSPLEVTLNVDG
                     EQGAVLPVRLESRVTEVGTLEVWCVSRDGKQRWKLELNIRDKSD"
     gene            3217..6147
                     /locus_tag="VFC15_10115"
     CDS             3217..6147
                     /locus_tag="VFC15_10115"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM10553.1"
                     /translation="MDEGAKGSGAVIDRASRYLIGVDLGTTNSVVAYIDTHGPPIEGS
                     SGIHVFEVPQLVGPGELRPLPHLPSFLYFPTEAEIASGTLVLPWEERPSSIAGAMARE
                     QGALLPGRQVSSAKSWLCQGAVDRTADILPVEAEPSAPMISPVEASARYLMHLRNAWN
                     HAMAADEASNPEQLFEKQEIVLTVPASFDEEARELTVEAARRAGLENLTLLEEPLATF
                     YAWIAEHQHAIRDQVQDGDLVLICDIGGGTSDFSLVRARSRGDEIEFERTAIGDHLLL
                     GGENLDLALAHRVEQKLHAKLSFRQRRGLQITCSAAKERLLTEPRIDRLPINILGSGR
                     AVVGQMLTSELTRDEVIELLTNGFLPLTAPDELPLRGRPSGLREIGLPYEGDPAITKH
                     LAAFLRQAALAMRPGASALSPTGDHPEMAIPDAVLFNGGFCVPEIARERITEAISRWF
                     GQKDHDWRPKVLRNDSMSSAVAAGAAYYGRVRRGTGLRVKAGSARSYYIGMRSESGGK
                     AVCVLPAGTDEGTTLPLAGREFSVLANRPVSFNLYSSTVRHDAHGDLADLDLGDMHRH
                     APLVTLLRYGKKLQELELAVRLAVSFTEVGTLELWCESVNSPHRWRLQFELRGKVDSP
                     QIGDSEPARTATTTLSSSSHVPEQALQSATQLLQTAFAKAPDGAATDPAPLVSELESA
                     IELRKESWPIAILRVLADALFEVADGRTLGPRHEARWLNLVGYCLRPGFGDAQDSRRI
                     NQARKIYQAGLTFPENLQSQVDWLVLWRRIAPGLSAAHQHELRNYLGGLGIGRKKPSA
                     RLNSQLEREGWRLLASLEHLSGATRAALGSELLRKLKKEPADGSWLWSLGRFGARIPL
                     YGSLNCVIPAATAADWITALLDLRELTHETASAILQLGRRTDDRARDVSDEVREFAIA
                     RLVKAGLADEMMVQRLKNFVGPDRDDVAHTYGEPLPKGLHLESTANCLSPVSALGAEP
                     GKLT"
     gene            complement(6206..6490)
                     /locus_tag="VFC15_10120"
     CDS             complement(6206..6490)
                     /locus_tag="VFC15_10120"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HZM10554.1"
                     /translation="MMKPVHTFTVVPSLPLAIENLREVAYNLSWCWSHDSISLFRRLD
                     SDLWETCGHNPVLLLGTIELCQQLLDLQDKRPRRRLTAVHKVFWVALRRF"
BASE COUNT         1418 a         1918 c         2040 g         1373 t
ORIGIN      
        1 tactgctccg gagcctcggg gccgtctttc gcgccgaagc cagcacgcat ccgatctcgc
       61 aagagaattg aatgaagctg ttcttaacgg ctccagcgca ggcgcgcaat cgcatagcca
      121 gactgtgcac ttgaatgtct cctggttatc tctacctccc agcacgaaac agtgagacta
      181 tcagttcacc actgcaaaca ggaatataat gaaccaggcc aatggactcg gcactcagga
      241 atgggagttg gttagcgtcg tcaaggtgca agtaacgaca aacgcctagc gagcgtttct
      301 taataggcca aagaacaact tctcagacga cttgcttcaa ccggttatct tcgtcacctt
      361 ggaactgatg tacattacat caccttgggt gaaccgaccg cgaatacgtg ctgagccgcc
      421 gtatcgagcc tgccttgcgg atgctgcgag ccgaggatgc tgaattggcg aacattcgtc
      481 atcagctagg agtttccagc gccaaccaat atgacgaaac attttgccgg gtaggcgttc
      541 ccgccattcg tttaccttca gcaagtctgg gccctctcac aaagtcgtgg atcgcggccg
      601 atctggcggg acagtgaagt tggcgtaagc cactgaaaac aagggttttg gaggggggct
      661 tcgatccagc ttgaggaagc cttccaaggt atgttctcct aagataatcg ctcggctagt
      721 gttgccagta gctggggagc catattatat gattggattt tggacacgac tctcttacgc
      781 agttcgttgt tttttcgcca tcctgttgca tgctgagatc ccgcaggaca tcacgcgcga
      841 gctaatcaag ccagcgcgcg atattgcgtc gcttggccga accgaatcgc ccagtacagc
      901 ggtaagtacg aaagagttgg acacttctgg caccgaatca gttgatcgag cggtgcagat
      961 gctcagcttg ctacaacgag atggacgcct tatcgatttt ctcgccgaag acgtagcagc
     1021 ctacccggac gcgcagctcg gagcggcagt tcgcagcatt cacacctcct gccgccaagt
     1081 ccttgaacga tatgtaaaac tcgatcctgt gatcgattcg gaagaggacc agccggtcac
     1141 cgtgccagcg ggcttcgatc ccgctgccat caagctgatt ggaaacgtca ctggcgaacc
     1201 acccattcgg ggtgtcctgc gacatcgcgg gtggcgggtg aaggaagtca cattgccatc
     1261 gctgcctcaa ggcgcaggac gagccatcgt cgctccagcc gaggttgaga ttccgtagca
     1321 aaatgcacac agtctcggat catgttcatt cagacagcac gtgatcctct tcatggagat
     1381 ttgattggat tcacaattca tcattggtat cgacctgggc accaccaatt gtgcgatggc
     1441 gcgctccagc atctcggctg cagaagcagg cagtggaatc gagctcgaga acatcccgca
     1501 actggttaac ccgaacgaag tcgctgaccg aacgctgctg ccatctttcc tttacatccc
     1561 gggcgaaatg gattttccca agggcagctt gaaactgcct tgggatgcgg agccgaaatt
     1621 tgtcatcggc gagttagctc gaaaacgagg cgccgaaaat cccgtgaggc tggtagcctc
     1681 agccaagtct tggctctcgt ataccgccgt caaccggacg gcgccgattc tgccctggca
     1741 ggcaccggag gaagtatcca agctgtcgcc gctggaagcc tcttcgcagt acctgcgata
     1801 tttgcgcact gtctgggata gcagccatcc cgagcaatcc ttgagcgagc aggacgtgct
     1861 gcttaccgtg cccgcttcgt tcgatgaaga agctcgtgaa ctgaccctgc gcgccgcaga
     1921 acaagcaggt ctgcaacacg tgacccttct ggaggaaccg caggctgcgt tttacgcttg
     1981 gctcgagacc caaggcgata gttggcgcaa gcgaatcaag gtcggcgatc tgattttggt
     2041 atgcgacgtt ggtggaggca cgacggattt cagcctgatt acggtctcgg aagagagcgg
     2101 cgatctgacg ctgaagcgcg tggccgtcgg cgatcacatt ctgctgggtg gcgacaatat
     2161 ggacctggcg ctcgcgcgat tgctgcagca gcgattggaa accgagggcc atcgggttga
     2221 tacgcaccat ctcctcggtc tgtggcacca gtctcggctg gcaaaagaat ttctgttcga
     2281 aaatccccag aagcagaagc atgacgtcac cttgttaggt aaaggacgaa agttagtcgg
     2341 cggcaccatc aagaccgaat tgctgcgtga agatgtggac cgtgtgctgc ttgagggatt
     2401 tttccctaag gtggggagcg acgagatgcc cctgcgccag cgccgcatcg gcttccagga
     2461 attgggactg ccatacgccg ccgatgcagc ggtgaccaag cacctggcgc gcttcctcgc
     2521 gcagcaagtg cagaacagtc cagaagccgc gaacattcgg cgtggccgca gcgggctggc
     2581 ttgtcccacg cacgttctgt tcaacggcgg tgtcatgaaa gcagcggcgc tgcgaagccg
     2641 gttggtggaa gtactgaatc tctggctggc cggggaaggg ttcgaggcgc ttggccgggc
     2701 gcacatcctg gatgcgcccg atctcgatca cgcagtagcg cgaggagcgg cttactatgg
     2761 ccgggcccgg cgtggacagg gagtcagaat ccgaagtggc gcctcgcgca cgtattacat
     2821 cgggatcgaa agcgctatgc cggctgtgcc gggcatggaa gccccgctga aggcgctttg
     2881 cgttgtgcca ttcggcatgg aagaaggaac tgaggcgcaa gtccccgatc gagagtttgg
     2941 cctggtagtt ggcgaacccg ccgagttccg ctttctcagt tcgacgattc gcaagcagga
     3001 ccacgtcggc gaccttcttg agaactgggg cagtgagatc gaggagctaa gcccgctcga
     3061 ggtaacgctc aacgtcgacg gagaacaggg ggccgttttg cctgtgcgtc tggagtcgcg
     3121 cgtaacggaa gtcggcacgc tcgaggtctg gtgcgtctcc cgtgacggga aacagcgctg
     3181 gaagctcgaa ctcaacattc gcgacaagag cgactgatgg acgaaggcgc caagggttct
     3241 ggggcagtga tcgaccgtgc ttcccgctac ctgattggtg ttgatctcgg caccacaaac
     3301 tcggtcgtcg cttatataga cacccacggg ccgccgattg agggatcatc cggcatccac
     3361 gtattcgagg tgccccaact tgtaggccca ggtgagctgc ggccgctccc gcatcttcct
     3421 tcctttctct atttcccgac ggaagctgaa atcgcatcag ggacgctcgt acttccctgg
     3481 gaagaacggc catcctccat cgcgggagcg atggcacggg aacaaggcgc gctgctgccg
     3541 ggacgccagg tttcatcggc gaagtcgtgg ctatgccaag gcgctgtaga tcgaaccgct
     3601 gacattttgc ccgtcgaggc ggagccgtcg gctcccatga tttccccggt cgaggcttcg
     3661 gcgcgttatc tcatgcatct tcggaatgcg tggaaccacg cgatggctgc cgacgaggcg
     3721 agcaatccgg aacagctttt cgagaagcag gaaattgttc tgacggttcc agcgtcgttc
     3781 gacgaagaag ctcgtgagct aaccgtggaa gcggcacgcc gcgccgggct cgaaaacctt
     3841 acccttctgg aagagccgct cgctaccttc tatgcttgga tagcggagca ccaacacgca
     3901 atccgggacc aagtacagga cggcgacctg gttctgattt gcgacatcgg cggtggcact
     3961 tctgatttca gcctggttcg cgcgcgctcc cggggcgatg agatcgagtt tgagcggacc
     4021 gccatcggcg atcatttgct gctgggggga gagaatcttg atctcgcgct ggcacatcgt
     4081 gttgaacaaa agctccatgc gaagctcagc ttccgccagc gccgtggctt gcaaattacc
     4141 tgttctgccg ccaaggagcg cctgctcact gaaccgagga ttgatcgtct tccgatcaac
     4201 attctaggga gcgggcgagc agtagttggt cagatgctca ccagcgagct gacccgcgac
     4261 gaagttatcg agctactgac aaacggcttc ttgcctttga ctgctcccga cgaattacca
     4321 ctacgcggcc ggccgagcgg gctgcgggaa attggcctgc cttacgaggg tgatcctgca
     4381 atcaccaaac atcttgccgc gtttctgagg caagcggcgc tggcgatgcg ccctggggca
     4441 agtgcgctct ctccgactgg cgaccaccct gaaatggcca tacccgacgc tgttcttttc
     4501 aacggagggt tttgcgtacc ggaaatcgcg cgcgagcgaa tcaccgaggc catatcgcgt
     4561 tggtttggcc agaaggacca cgattggcgc ccgaaagtcc tacgtaacga ctcgatgagc
     4621 agcgcagtgg cagcgggggc cgcgtactac ggtcgcgtgc ggcgcggaac tggtttgcgt
     4681 gtcaaggcgg gcagcgccag gagctactac attgggatgc gctcggaatc cggtggcaag
     4741 gctgtttgcg tgctaccggc gggaacggac gagggcacaa ccctcccgct cgcgggacgt
     4801 gaattttcag tcctggccaa ccggccagtg tcatttaacc tctatagctc gaccgtccgg
     4861 cacgatgcac acggagactt ggccgacttg gacctcggcg acatgcatcg gcatgcgccg
     4921 ctggtaaccc tgttacggta cggcaagaaa ctacaggaac tcgaactcgc ggtgcgcttg
     4981 gcggtgagct tcacggaggt cgggacgctt gagctctggt gcgaatcggt gaactcgccg
     5041 catcgttggc gactgcagtt tgaactacgc ggaaaagttg attcgcccca gatcggcgac
     5101 tctgaaccgg cacggaccgc gacgacaact ctgtcatcgt cttcgcacgt tcccgaacaa
     5161 gcgctgcaat ctgccacgca actgttgcag acggcattcg ccaaggctcc cgatggcgca
     5221 gccaccgacc ctgcgccgct ggtgagcgaa ctggaatccg ccattgagct cagaaaggaa
     5281 tcctggccca tcgccattct tcgagtgtta gcagacgcgc tcttcgaagt tgccgatggc
     5341 cgaacgctgg gcccacgtca cgaagcgcgc tggcttaacc tcgttggcta ttgcttacgg
     5401 ccgggcttcg gagatgcgca ggacagccgg agaatcaacc aggcaagaaa gatctatcaa
     5461 gctgggctga cgtttccgga aaatcttcag tcccaagtcg attggctggt gttgtggcgg
     5521 cggatcgccc cgggcctgag tgccgcgcat cagcatgaac ttcggaacta cctcggcggg
     5581 cttggcattg gacgcaagaa gcccagtgcg cggttgaact cgcaactgga gcgcgaagga
     5641 tggcgtctgt tggccagtct cgagcacctc tccggcgcta cgcgtgccgc gctggggagc
     5701 gaattgctgc gcaagctcaa gaaggagccg gccgacggca gctggctgtg gtcgttgggc
     5761 agatttgggg cacgcattcc actctatggc tctctcaatt gcgttatacc ggcagcaacg
     5821 gcggcggact ggattacagc gctcctggat ttgcgcgagt tgacgcacga gactgcatcc
     5881 gcaattctcc agcttggcag gcgtaccgat gaccgcgcgc gcgacgtgag cgacgaggtg
     5941 cgtgagtttg cgattgcgcg gctggtcaag gccggcctcg cagacgaaat gatggttcaa
     6001 cgcctgaaga atttcgtggg ccccgatcgc gacgatgtgg cgcatactta tggtgagccg
     6061 ctgcccaagg gcttacacct ggaaagtacg gcaaattgcc tgtcgcccgt ctcagcactg
     6121 ggcgccgagc cagggaagtt aacgtagtcc cgtagttcgc cagaactgaa gacagccatt
     6181 aagacgggag gcttctccac ccggatcaga accttctcaa cgcgacccag aacaccttgt
     6241 gcacggcagt caatcgacgg cgaggtcgtt tgtcttgcag atctagtaat tgctgacaga
     6301 gttcgatggt gccgagcagc agcacagggt tgtgcccgca ggtttcccat aggtcgctat
     6361 ccagccggcg gaagagtgag atcgaatcgt ggctccagca ccaactcagg ttgtaggcaa
     6421 cctcgcgcag gttctcgatc gccagcggca acgagggtac gacggtgaac gtatgaacag
     6481 gtttcatcat ggccgaacct acgatcttcg ttgccggcca tcgatcaggc gggaagcttg
     6541 cgtccctcgc cttgatgaac tgcggcgagc gtcattatct tccatgcttg attgagctgt
     6601 cgcgggaccc gtcggcatca gacggagacg agattagaac catgccttgg cccggccgta
     6661 agtcatagga aaaccgttct gtggagatat aatccagtgc gtactccggg gacacatctc
     6721 taagcggtgc tcccgatttc atcagtgcg
//
LOCUS       DASRKK010000434         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_229127, whole genome shotgun sequence.
ACCESSION   DASRKK010000434 DASRKK010000000
VERSION     DASRKK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229127"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1298)
                     /gene="nikB"
                     /locus_tag="VFC15_10125"
     CDS             complement(378..1298)
                     /gene="nikB"
                     /locus_tag="VFC15_10125"
                     /inference="COORDINATES: protein motif:HMM:NF045470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HZM10555.1"
                     /translation="MMPRYLLSRSLYMLPVVWLVVSVVFLLIHLVPGDPIQQMLGEGA
                     AATDIAAARHAYGLDAPLGQQYVNYWRGLVHGDMGRSLRLNQNVGHVIAQAYPATMEL
                     TLAALLVALCLSIPAGVRSALRRNRWDDRALSFVSLLGLSFPNFALGPILILFFAIEL
                     GWLPVSGSGSLAHLVLPAITMGGALAAILTRMIRTSMLEELGQDYIRTARAKGLSERT
                     VVYRHALRNAMVPILTVVGLQFGALLAGAIVTETIFSWPGIGRLTIQAISNRDYYLVQ
                     GCILMIGLTYVLVNFITDLLYSAFNPRIRQ"
     gene            complement(1295..2680)
                     /locus_tag="VFC15_10130"
     CDS             complement(1295..2680)
                     /locus_tag="VFC15_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZM10556.1"
                     /translation="MVTFVVLRIVVVVLLVAANAFFVAAEFALVNVRETRLQQMIAAH
                     RLGARTVQKLHQKLDHVLNTVQFGVTVASLALGWIGEASMARILEPLFLPLPHSRLYA
                     HGVSVVVAFALITYLVVILGEVVPKTIALQRTERVALAVAGPMDFFMTVAAPFLAFMT
                     ASTRVALRAFGMRQVREGGVHSSEELKLIVSASRRLGMLAPTQEELIHRALELENISV
                     REIMVPRRDIFSLPGDISLEEALQRVVDEQHSRVPVYDVQRGPEHIIGVLYAKDLMRW
                     LRYRVNRTASGRPVTRPSGLQVRHIMREVLVVPETKPLADLLIEFKIRKRHLAVVVDE
                     FGSTAGLVTVEDVLEQLVGEIEDEFDITEPRLAPGAASMALDGSVNIRDLESQYHIAL
                     PRDEGFETLAGFMLAQLQKIPEVGDSFEYQGRRYTVAAMDGLRVVSVNIEGTEPQSQV
                     QSSPATPQAQG"
     gene            complement(2787..3119)
                     /locus_tag="VFC15_10135"
     CDS             complement(2787..3119)
                     /locus_tag="VFC15_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10557.1"
                     /translation="MPPRKLVQIADTPNLPTIKDYFGMMAEEQAEDIRRLKQEVTGKN
                     SGQPEKQPADEAVPDPWLLHPIRPAKGGAKRGAKGTTSSATKKAPKKAAKKAAKKAAK
                     KTSKKARR"
     gene            3311..3931
                     /locus_tag="VFC15_10140"
     CDS             3311..3931
                     /locus_tag="VFC15_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10558.1"
                     /translation="MFITQFVVIRKSKELAVLQGQDSQDKDVKIAAAHERAANLEKEA
                     AALLKQLIEQGPRSHLLYGERRERLIAQLKPFSGQKAEIRFCNSSFNLYSVDNDTTSV
                     AMLLKYILEQANWSVNPLVIDNCSGTGLSVSIHPIAPTSAHQAADALLSALLSLPLTV
                     VGNKVFVEESPRPPQPRVLDHDNNGKEIGLNPLGHDTVVVRVLAHP"
     gene            complement(3993..>4188)
                     /locus_tag="VFC15_10145"
     CDS             complement(3993..>4188)
                     /locus_tag="VFC15_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10559.1"
                     /translation="PYRCPIAPETRKMKRKLWHLLYGKKPNVYRVIYEVDERRHTVWV
                     LTIRHGARRKVEGSELGSRS"
BASE COUNT          915 a         1237 c         1212 g          824 t
ORIGIN      
        1 cgattcgaat tggttgcccg ggccgccggc caaaaatcat ggtcagccgg ctccgtccat
       61 gccgaagcaa tacggtttcg gcggatgtcc gttcgcggta attgatgttc cccagcgaac
      121 actggggcag cgtgattcta gagcagtttc agacttggtg tatagggtgg agcagcgctt
      181 cagcgctgca gtttgagatt cggaaatgag ctggctttag ccaccggggg gacggaaaca
      241 tcacctcagg cgctaaagcg cgatccagga actggcggcc gttatgcacg cctgaaaggc
      301 gtgctccacc catcgcctat acaccagaca tgaaactgct atagcgctgg gttggtctga
      361 gtctggtggc agctccgcta ctgccgaatc ctcgggttaa acgccgaata caacagatcg
      421 gtaatgaagt tcaccagcac ataggtcagc ccgatcatca gaatgcagcc ttgcaccagg
      481 taatagtcgc ggttggagat ggcctgtatc gtgagccgcc caatgccagg ccacgagaaa
      541 atcgtctccg tgacgatggc tccggcaagc agcgctccaa attgcaatcc gacgacggtg
      601 agaatcggga ccatggcatt gcgcagtgcg tgccggtaaa ccacggtgcg ttcggacaaa
      661 cccttagcgc gcgcggtgcg gatgtaatcc tgtcccaact cttccagcat ggaggtgcgt
      721 atcatccgcg taagaatggc cgccagtgct cctcccatcg tgatcgcggg aagcaccagg
      781 tgggccagcg agcccgagcc tgagaccggc agccagccca attcaatcgc gaaaaacaag
      841 atgaggatcg gccccagcgc aaagttcggg aatgacaatc ccagcaggga gacgaagctg
      901 agcgcccgat cgtcccaacg attgcggcgc agcgccgaac gtacgccggc aggaatcgac
      961 aggcagagcg ccaccagcaa tgccgcgaga gtgagttcca tggtggcagg ataggcctgt
     1021 gcgatcacgt ggccgacatt ctgattgagg cgcagcgagc gtcccatgtc gccgtgcacc
     1081 aggccgcgcc agtagttcac atactgttgc cccagcggag catccagccc gtaggcatga
     1141 cgcgccgcgg cgatgtcggt ggcagccgct ccttcgccca gcatctgctg gatgggatcg
     1201 cccggcacta gatgaatcag caggaagacc accgacacca ccagccagac gaccggcagc
     1261 atgtacagcg atcgcgagag cagatagcgc ggcatcatcc ttgggcctgg ggcgttgccg
     1321 gggacgattg tacctgggac tgcggttctg tcccttcgat gttcacgctg accacgcgca
     1381 gtccgtccat ggccgcgacg gtgtagcggc ggccctggta ttcaaaactg tcgcccacct
     1441 cgggaatctt ctgcagttgc gccagcatga agccggcgag cgtttcgaag ccttcgtcgc
     1501 gcggcaacgc aatgtgatac tgcgattcca gatcgcgaat gttgaccgag ccatcgaggg
     1561 ccatcgaggc agcgcccggc gcaagccggg gctcggtgat atcgaactca tcctcgattt
     1621 cgccgaccag ttgttccagc acatcttcca ccgtcaccag gcccgcagtc gagccgaact
     1681 cgtccactac cactgccagg tgccgcttgc gaatcttgaa ctcgatcagc agatcggcaa
     1741 gcggcttggt ttcgggcacc accagcacct cgcgcatgat gtggcgcact tgcaggccac
     1801 tgggccgggt gactggtctg ccggacgcgg tgcgattcac ccggtagcgc aaccagcgca
     1861 tcaggtcctt ggcatacaac accccgatga tgtgctcggg gccgcgctgc acatcgtaaa
     1921 ccggcacgcg cgagtgttgc tcgtccacca cgcgctgtaa cgcttcttcc agcgaaatgt
     1981 cgccaggcaa cgaaaagatg tcgcggcgcg gcaccatgat ttcgcgcaca gaaatgtttt
     2041 ccagctccag cgcgcgatgg atcaattcct cctgcgtggg cgccagcatt cccagccgtc
     2101 ggctggcgga gacaatcagc ttcaactcct cggacgagtg cacgccgcct tcgcgcacct
     2161 gtcgcatgcc aaacgctctc aaagcgaccc gggtggaagc ggtcatgaaa gcgagaaacg
     2221 gcgcggcaac ggtcatgaag aaatccatcg gtccggcaac tgccagcgcc acgcgctccg
     2281 tacgctgcaa ggcaatcgtc ttgggaacca cctcaccgag gatgacaacc agataggtga
     2341 tcaacgcgaa cgcaaccacg acggagacgc cgtgcgcata gagccgggag tggggcaatg
     2401 gaaggaacag cggttccaga atgcgcgcca tcgaggcctc gccgatccag cctagggcta
     2461 ggctggccac cgtgacgccg aactgcacgg tgttgaggac atggtcgagc ttctgatgga
     2521 gcttctggac ggtgcgcgcg cctaggcggt gcgccgcgat catttgttgc agccgcgtct
     2581 cgcgcacgtt caccagagcg aattcggcgg ccacgaagaa ggcgtttgcc gcgacgagca
     2641 gcaccaccac cacgatgcgc agcacaacga aagtaaccat gaagctgaaa tcttaccatc
     2701 ggcgggaagg cgggagcggc tcgcaagcgc cggaggaaaa gctggcgcta gcgctcacac
     2761 cgggcaatct tgcttgcgga ggagatctac cgccgcgctt tcttcgacgt cttctttgcc
     2821 gctttcttgg cggccttttt tgctgctttc ttgggagcct tcttggtcgc cgagcttgta
     2881 gtgccctttg cgccgcgctt tgccccaccc ttggcggggc gtatgggatg caatagccat
     2941 ggatctggta ccgcttcgtc ggcgggctgc ttctccggct gccccgagtt ctttccggta
     3001 acctcttgtt tcagcctgcg aatgtcttct gcctgctctt cggccatcat gccgaagtaa
     3061 tccttgatgg tgggcaggtt gggagtgtcg gcgatctgca caagctttcg tggtggcatg
     3121 ctcagctaag ataccgcgat tgcattagga tgtcactgct tggtagtgtc gagcaagcaa
     3181 aagaaagggg aaagacatgt cgtcaatcca gcccctcagc gatcatatcc aagacttagg
     3241 gcggtgaatc gacaagtgga actcttggta tatcttctta gtcgctgcca ccgtaatctt
     3301 ggcggctttc gtgtttatta cgcaattcgt tgtcatccga aaaagcaaag aattagcggt
     3361 gttgcagggg caggattccc aagacaaaga tgtgaagata gctgccgccc acgagcgggc
     3421 ggcaaatctt gaaaaggaag ccgccgccct attaaagcaa ctcattgaac aggggccaag
     3481 gtcgcacctc ttatatggcg aacgacggga acggcttatt gcgcagttga agcccttttc
     3541 gggccagaag gcagagatta ggttctgtaa ttcctcgttc aatctgtatt ccgtcgataa
     3601 cgacacgacg agcgtagcaa tgttactgaa gtacattctg gaacaagcca actggtcagt
     3661 aaaccctttg gttatagata attgcagcgg aactggattg tcagtgtcaa tacatccgat
     3721 agccccaacc tccgcccacc aagccgcaga tgccctactt tccgcattgc tatcactacc
     3781 actcactgtc gttgggaaca aggtttttgt tgaggaatct ccccgcccac cgcagcctcg
     3841 ggtgttggac catgacaata acggtaaaga gattggcctt aatccgctgg gccatgacac
     3901 tgtggtcgta agagtgctcg cccacccatg aacgcccagc gctagtgcaa gaaggcaaat
     3961 taggacacta cccactgccc tgcaagggga cattatgaac gcgatccaag ctcggacccc
     4021 tcgacttttc gccttgcgcc atggcgaatc gtgagcaccc acaccgtgtg ccgccgttca
     4081 tcgacttcat aaatgactcg ataaacgttc ggcttcttgc cgtaaagcaa atgccacaac
     4141 tttcgcttca tcttccgagt ttccggggca atagggcagc gataagga
//
LOCUS       DASRKK010000435         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1403391, whole genome shotgun sequence.
ACCESSION   DASRKK010000435 DASRKK010000000
VERSION     DASRKK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403391"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFC15_10150"
     CDS             <1..256
                     /locus_tag="VFC15_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10560.1"
                     /translation="CGAAIVVPIAAGGLTEQANRLAASSCQFCHTPWEFTPNSNEHKL
                     LRDFASALEQIAAQLKGRNLKLNLEIKCPEQVGTYAKSKE"
     gene            724..1848
                     /locus_tag="VFC15_10155"
     CDS             724..1848
                     /locus_tag="VFC15_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10561.1"
                     /translation="MVSLLPYIEADLIRCGVRRLDDVSQTHLAACWKSLKKRFPSRAS
                     TARVLGVYLRTLGLVKPEERRVLGAGEEVLTSYVRHLEGVRGAARTTIQHHLRTAAAF
                     LVHSKIAKHNGRLSTPTVSDVEGFVKKSGQRLNRASLKQTVAKLRGFLRFLATRGDAP
                     PGLDRQIDSPRVYREEQLPRTLPWDTVQTLLRSIDCSSLLGLRDFTIFLLMATYGLRT
                     SDIVALTLDDIHWRAGKISISQRKTGTVLELPLTDAVGTALLKYLKRVPPQPPFRQIF
                     LRMRAPVGTLKTPTISQAFYGWVRRSGLQIPLQGPSCIRHSYAVFLLRSGTPLKTIGD
                     LLGHRSFESTCTYLRLAIEDLRDVALPVPSVRKGNREVRI"
     gene            2115..2852
                     /locus_tag="VFC15_10160"
     CDS             2115..2852
                     /locus_tag="VFC15_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10562.1"
                     /translation="MPNCFVPDPIFFPSSHQAAAPYVFSESQIAQLLTASSGLRRPPD
                     SPLRPEMIRLAIVLLYSTGLRRGELLRLTIGDFEIRREGTLLVRASKFHKSRILPLPQ
                     DVIGEIDFYLRMRRQHNLPVLPNTPLIWNRRNGGRAYTGFGFQTSLMPLFDVCSIHTT
                     AGRPPRIHDFRHSFAVNALIRWYRAGCDVQTKLPFLAAYMGHVSIVSTYHYLHFIEPL
                     RTLASNRFCDCYGGLVTPVSMQNGGRA"
     gene            2849..3862
                     /locus_tag="VFC15_10165"
     CDS             2849..3862
                     /locus_tag="VFC15_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10563.1"
                     /translation="MKITVPNNLAFRLRAYFTDHLPRLRGVSAHTVHSYRDSLLLLLR
                     FLASRRHCHVADLDLGDIEPDHVIAFLAHLEEDRKNGVPTRNVRLAAIHAFFRYIGAC
                     HPEHLEQVQRIVGIPFKRARQRVIEYLERSEIEVILSRIDRANRDGRRDYALLATMFN
                     TGARVQEIVDLRARDLQLIRPFQIRLFGKGRKERFCPLWPQTARVLRDHCEERHMDLR
                     SDSRVFLNHREEPLTRFGVRYILAKHLDRVRDDVPTLARKRLHPHSMRHSTAVALLKS
                     GVDLSSISQWLGHASLNTTNRYASVDLEMKRRAIARVKPIANGGTAKWRRSQSILEWL
                     ETL"
     gene            4252..4620
                     /locus_tag="VFC15_10170"
     CDS             4252..4620
                     /locus_tag="VFC15_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM10564.1"
                     /translation="MTGRTVEIGDEFEPEFNELHEDVRTEVLALSRVLQQFGPQLGRP
                     RVDTLNGSRHANMKELRFSATDGEWRVAFAFDPKRKAILLVGGDKSGVSEKKFYRQLI
                     AKADDRFDAHLARQRKKERT"
BASE COUNT         1026 a         1328 c         1237 g         1065 t
ORIGIN      
        1 gtgtggggcg gcaatagttg ttcctattgc tgctggcggt cttacagagc aagcaaatag
       61 acttgccgca tcttcgtgtc aattctgtca tacgccttgg gaattcacgc ctaatagcaa
      121 tgaacacaaa ctcctccgag attttgcctc tgcacttgag cagatcgccg cacaattgaa
      181 ggggcgcaac ttgaaactaa acctagagat aaagtgtccg gagcaagttg gaacatatgc
      241 caaaagcaaa gaataagaag tcaccacctc gaccagcatc caagacaggt ccgaaaccgg
      301 acatgctaaa actctctggc aactggaagc aggcggtcaa gaaatcgttt cagaagaaga
      361 aaccagcgga gggatggccg aagtagagtc gccattttac gcgactcacc gccgcttacc
      421 gcgactcgcc gccatcgcga gttgcgtaaa gtacatactt ccgttgatta atgttgagaa
      481 ctcagtaatg tggagttctc ccttgtaatc gccgtggttg ctggtctccc aaggaaactg
      541 ctgcacattt cctcgtagca tcggatcggc gctgtctagc gccgaaaagg agatctgatg
      601 cttacaactc tcttcccaca gagcttccga aaatattctt ccttggcgat actcgggtcc
      661 gttgcggacg ggtttgccag ttggctcgtg gagcacaagt acacccgcag aacgaggcgg
      721 cagatggtta gcctgcttcc ctatatcgag gcggacctca ttcggtgtgg cgtccgccgc
      781 ctcgacgacg tcagccaaac ccacctggcc gcctgctgga aaagcctcaa gaagcggttc
      841 cccagtcgag ccagcacggc tcgggtgctg ggagtgtatc tacgtactct cggtctcgtg
      901 aaacctgaag aacggagggt gcttggtgca ggcgaggagg tgttgacttc ctacgtgcgc
      961 catctggaag gcgttcgagg agccgctcga actaccattc agcaccatct ccggacggca
     1021 gcggccttct tggttcactc caaaatcgcg aagcacaatg gccggctttc gaccccgaca
     1081 gttagtgatg tcgaaggttt tgtgaagaaa agcggtcaaa ggttaaatcg agcgagcctg
     1141 aagcaaacgg tcgctaagct gcgtggtttc ctccgattcc tggcgacaag aggcgacgca
     1201 ccaccggggc tcgaccgtca gattgattca cctcgtgtct accgcgagga acaactgccc
     1261 cgcaccctgc cgtgggatac ggtgcaaaca ctcctcagat caattgattg ctccagcctg
     1321 ttggggctcc gtgatttcac gatatttctc ctgatggcaa cctacggact acgcaccagt
     1381 gacatcgttg cgttgacctt ggatgatatt cactggcgag ccggcaaaat ctccatttcc
     1441 cagagaaaga ccggcactgt tctcgaactt cccttgaccg atgccgtggg cactgcgctg
     1501 cttaaatacc ttaagagagt tccaccgcag ccgccgttta gacagatctt ccttcgcatg
     1561 cgtgctccag ttggaaccct caagacacct actatctcgc aagcgtttta cggctgggtg
     1621 cggcgaagcg gattacagat tcccttgcag gggccaagct gcattcgtca ttcgtatgct
     1681 gtgtttctgc tgcggagcgg cactcccctg aaaaccattg gcgatcttct tggccatcgc
     1741 tcttttgaaa gcacgtgcac atatttgcgc ttggcgatcg aagacctgcg tgatgtggct
     1801 ttgcctgtac cgtcggtccg aaagggcaat cgagaggtga gaatatgacg cagatatcgg
     1861 cgtttacgtc cgcgctgcgc tcgcccatca agtcctacct tgagctgaag ttcgcccaag
     1921 ggtaccggtt cgaccgcggg ctcgctgttc tgagatccgt cgatcgatta ttgagcgggc
     1981 ttcccagggc ctgccaagat ctgaccgaag agacctttcg ttcctggtgt acagcacaat
     2041 ctgagatatc acctggcgag cgacggcgcc gaatgaacat aattcgcaac ttctgtctct
     2101 atcgtcgtcg aactatgccg aactgctttg tcccagatcc tatctttttc ccgtcttctc
     2161 accaagccgc ggcaccgtat gttttttcgg aatcccagat tgcacaacta ctgaccgcct
     2221 cgtcagggct tcggcggccg cccgattcgc cgcttcgccc ggagatgatc cgtctcgcca
     2281 tcgtgcttct gtactccacg ggtttgcgcc gtggtgagtt gcttcggctt acgatcggtg
     2341 acttcgagat ccgccgcgaa ggcacgctcc tcgttcgtgc ctcgaagttc cacaagtcac
     2401 gcattcttcc gctgccccag gatgtgatcg gcgagattga tttctacttg cgaatgcgac
     2461 gccagcataa cctgcctgtt cttcccaaca cgccgctaat ctggaatcga cgaaatggcg
     2521 ggcgagccta tacaggattc ggattccaga ccagtctgat gcccctcttc gatgtctgca
     2581 gcattcacac gacagctggc cgcccgccgc gaattcacga ttttcggcac agttttgcgg
     2641 tcaacgcgct gatccgctgg tatcgcgccg gctgcgacgt gcagacgaaa ctgccattcc
     2701 tggcggccta tatggggcat gtctccatcg tgtcgacata ccattacttg cacttcatcg
     2761 agcctctccg cacccttgcc agtaaccggt tctgtgattg ctatggtgga ctcgtcactc
     2821 cagtgtcgat gcagaacgga ggaagagcat gaaaatcaca gtaccgaaca atctcgcctt
     2881 ccgtctgcgg gcttacttta cagatcacct gccccgactt cgcggcgtga gcgcccacac
     2941 tgtgcacagc tatcgtgata gcttgctact gcttcttcgg ttcttggctt ctcgaagaca
     3001 ctgccatgtc gcggatttgg atcttgggga cattgaaccg gaccatgtta ttgccttcct
     3061 ggctcatttg gaggaggacc gcaaaaacgg tgtgccaacc cgaaatgtcc gcttggctgc
     3121 tatccacgct ttcttccgct acatcggagc ttgccatcct gagcacctcg agcaagtcca
     3181 gaggattgtc ggtattccgt tcaaacgggc ccgccagcgc gtgatcgaat acctggaacg
     3241 ctccgaaatc gaagtcatcc tttctcgaat cgaccgcgca aatcgggatg gccgacgaga
     3301 ctacgctcta ctggcgacca tgttcaacac gggagcacgc gtgcaggaga tcgtcgatct
     3361 gcgtgctcgc gatcttcaac tgatacgacc gttccaaatc cggctctttg gcaaaggcag
     3421 gaaagagcgc ttctgtcctt tgtggccaca gaccgcccgt gtccttcgcg atcattgcga
     3481 ggagcgccac atggacctgc gctccgattc gcgggtcttt ctcaaccatc gcgaagagcc
     3541 gctaacccgg tttggggtgc ggtacatctt ggcgaaacat ctcgaccgtg ttcgcgacga
     3601 tgtccctact cttgcgcgca aacgccttca cccacacagc atgcgtcaca gtacggctgt
     3661 agcccttctg aagtcaggtg tggacctttc atcgatcagt cagtggttgg ggcatgccag
     3721 cctgaacacg acaaaccgtt acgccagcgt tgatttggaa atgaaacgcc gggccattgc
     3781 tcgagtcaaa cccatcgcca atggtggaac cgccaaatgg cgcaggagcc aatcaatcct
     3841 ggaatggttg gagacgctct gaactgctca gtaatgtgga ggtctaagcc ctgaaaccgc
     3901 ggctgtagcc agaaactttt aactcactcc acattactga gctctcaaca ttaatcaccg
     3961 gtgaccaggg cgaattgttc aaggcccttc cgatgcccat tcagccagcg attgatgcca
     4021 atgctactga tccggttgga gcgctcggag aacttatcca gatgggagat gcgcccgcct
     4081 gcaacatctg cggttcgctc atggtacgta gcggaacctg ctaccgctgc atgacctgcg
     4141 gcagtacaag cggttgctcg tagccgcaca tccgcaatcg ttttcgttca ccaatctaga
     4201 ataatgcact tgacggcatt atgcctcaca gggcatagta ggaatattcg tatgacggga
     4261 cggactgttg aaatcggcga cgagtttgag ccggaattca acgaactgca cgaggacgtg
     4321 cgtaccgaag tcctcgctct gtcgcgggtt ttgcaacagt tcggaccgca attgggaagg
     4381 cctcgcgtcg acacgctcaa tggctcgcgc cacgccaata tgaaggagct gcggtttagt
     4441 gcgactgacg gcgagtggcg agtcgctttc gccttcgatc caaaacgcaa agccatcctg
     4501 ttggtggggg gagataagtc tggcgtgagc gaaaagaagt tttatagaca gctcattgcc
     4561 aaagccgatg atcgatttga tgcgcatctt gcccgtcaaa gaaagaagga aaggacgtag
     4621 ttcatggcca ggaacgtgaa cgacattatt gagagg
//
LOCUS       DASRKK010000436         5736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_328124, whole genome shotgun sequence.
ACCESSION   DASRKK010000436 DASRKK010000000
VERSION     DASRKK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328124"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            725..1051
                     /locus_tag="VFC15_10175"
     CDS             725..1051
                     /locus_tag="VFC15_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM10565.1"
                     /translation="MKTARLTIAVCSLFFALTVVSAQTPLSDTSLTANPVFQKTCAKC
                     HGKTAEGRHFGGPSLVSEKTTAASPDDVRNPISNGKGRMPKYAGKLTSEEIDTLVQQI
                     KALNKK"
     gene            1215..1385
                     /locus_tag="VFC15_10180"
     CDS             1215..1385
                     /locus_tag="VFC15_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10566.1"
                     /translation="MNCAFVANYTRVFTPTLLNRFSFGENNFINITNPVVSSPQIDLP
                     SLQEGASFRVPQ"
     gene            1439..1777
                     /locus_tag="VFC15_10185"
     CDS             1439..1777
                     /locus_tag="VFC15_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10567.1"
                     /translation="MLHSKLHELWSRRKGTQVREAESGFRYTPTSTFETFPFPWPPGH
                     EPKDSPLAEAIAQAAGQLVEKRDAWLNPPGASADDLKTRTLTNLYNARPAWLEDLHRA
                     LDNAVFAAYG"
     gene            complement(1862..2890)
                     /locus_tag="VFC15_10190"
     CDS             complement(1862..2890)
                     /locus_tag="VFC15_10190"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZM10568.1"
                     /translation="MNNIFISYRRTDSQDATGRIHDHLDMYFGPEVAFRDIDRLHAGT
                     DFPEELNKALAQCSLVIGVIGDQWLSVTYKDGTERLKDPKDLVRIELATALFRGVPVI
                     PVLTGNAGMPSAEQLPPDLERLAYKQAIHIPSSRGFVRGVRQLVEEIHQLTALPFEDF
                     PSVITDCQRAGLVLVKDNFREDTSVLDEMKTTRELLVVMNDGRGWLDQNRDIIYGRLR
                     DPDKVTRVVLLHPASPFLATLIRKNRKTLATQIEEIKRSYESLQNYEKSQGQLYIRGH
                     MLFNGYSLTLGDRYAFVSPYFFNESGALPLLKFASRVGQGLYQQLRADAMRLFDTAVP
                     LTQEDFRS"
     gene            3166..3687
                     /locus_tag="VFC15_10195"
     CDS             3166..3687
                     /locus_tag="VFC15_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM10569.1"
                     /translation="MQTYHRVHVQLGKRDRQQIVGMLNKGRESARVLRRALILRQLNQ
                     GQTAAQVAGNVGVAPKTVRAIARRYAEEGLDSALYEKPRPGKQRRLEAGQSQRIIAMV
                     CSEPPAGQARWSVRLIAQEAVRRKLVPKVGRECIRILLKSHDLKPWREKKLVRGGTES
                     GIPAAHGRGARPV"
     gene            3629..4297
                     /locus_tag="VFC15_10200"
     CDS             3629..4297
                     /locus_tag="VFC15_10200"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM10570.1"
                     /translation="MAELNQEYLRRMEEVLALYEKPLSEKQPVVCIDEKPVVLHQDVR
                     PPLPMRPGRIARRDCEYQRCGTANAFCGVEPKAGRHFTRATPDRSSPRFADYLLAIAE
                     YYAAADTIHLVMDNLSSHTRKAVVERFGEKAGGWLWDRFTVHYTPKHGSWLNQAEIEI
                     SLFSRQCLGQRRIPSLRALRREARAWNRRINRQRVTINWRFTPTKARAKFGYKRNQIT
                     RSET"
     gene            4399..5619
                     /locus_tag="VFC15_10205"
     CDS             4399..5619
                     /locus_tag="VFC15_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10571.1"
                     /translation="MGVFAATDWLVAGLNYRHYGIFETNETRSGSFLRAYGALSRIQH
                     NQWHRYISFPKDVRLHAYAASPAARELETSLEGPTGDKWLQVTCSLSPVKITPCDEVQ
                     AGWSMWEFREAVASAGHYRSGREAMRFYDTLANQINSACERGTIQCLPRRSTLSPPFR
                     REYLGETVEAGKVEAKVMFTMGDGQVGSIASIGPPEGIATFADTVGGVDLPDERVRVV
                     QGWAAAASATPTVRLLAHTPVPVQSSISITPAPDVLAVYPDLKAIRFELTTDCPVADC
                     DLVLDIAGGDQPPVPLARLVHGAVIDTPQLRVYVDSAAVGEGTKLTDSRRAVQLKIAR
                     LIASAYAKAFPILAIFGAGGLLFATFFRRWCPIPTGLLALGLGSAAAVLSRIVLLSYL
                     AATSFPGANRPWCK"
BASE COUNT         1243 a         1643 c         1659 g         1191 t
ORIGIN      
        1 aattgtacgg tagcgttggt tgtggagaat ggtttctggt cggtgaagcg gaggcaggct
       61 ctgccggagg gcaacctgcc aaggaatagc cgactcgaaa ctcatcgaag ccgatgagca
      121 ccgggagcga cgataatgcc agttgctccc ggtcttgccg agttcctaca tcggatcgat
      181 agatttcctc atgcctaaga aaactccctt gcacacgcgc tgatccggcg tgcagggcag
      241 gatccgctct acgcgaggaa gattcgcagg caactcctgg cggcccggat gcttgcagga
      301 tctgttcgcc gagcgccgca ccagcgcgcg ctgcgggagt ctttggagac acttggagga
      361 gtttgcggac gtgggcgagt ttgtagtact cgccccagcc ccgcaggagc ggattaggcc
      421 tcgcaatcag ttgctcggtt cgcagaggtt gtcgtccgtt tggtgcgttg ccgtacctgg
      481 tccatgaaac gacgaatcga cttctccttg ggatacgcat atagcgcacc ctgtcgggcc
      541 tgactacgaa tcttgctctc gggcagagaa agcttcttcc agccggactt gattttgtag
      601 ccgaggaact cgaagccgta tcggacgcga acaatccgcg tgttctgtcc ctgctcacag
      661 ataccccgag atctttcaat gcatctaagg tgcctaacgc aaatcaatcc cgacgaggtt
      721 cgacttgaaa acagcccgac ttaccatcgc cgtgtgttct ttattctttg cactcactgt
      781 agtctctgcc caaaccccgc tgtccgacac gagcctgacg gcgaatcccg ttttccagaa
      841 aacctgcgcg aagtgtcacg gtaaaaccgc ggagggccgc cacttcggtg ggccgtcact
      901 cgtctccgaa aaaaccactg ccgcatcgcc cgacgacgtg cgcaacccca ttagcaacgg
      961 caaaggccgc atgcccaagt acgcgggcaa actaacatcg gaagaaatcg acacgctggt
     1021 ccagcaaatc aaagccctaa ataagaaatg aatttccgat agggttcgtt gttcctggcg
     1081 ttggcatcgg tcggaaagga accctgccgg ataagaaatc agtgccgcca ccaaattgaa
     1141 cccggcataa atcaggatgg tcagttcaac cgaagcgcca atgctctttg tttgcagaat
     1201 cagaaacaag gtacttgaat tgcgcgttcg tcgccaacta tacgcgcgtc ttcactccaa
     1261 ctctgctcaa ccggttcagc ttcggcgaaa acaatttcat caacatcacc aacccagtgg
     1321 tgagctcgcc gcagatcgat ctccccagcc tgcaggaagg cgcatcgttc cgcgtgccgc
     1381 agtagaccaa gcagaaccgg ctgccgccat agcacgtgac gatgattact ttattggcgt
     1441 gttgcattcg aaattgcacg aactttggtc aaggaggaaa ggaacacaag ttcgtgaagc
     1501 ggaaagcggt ttccgttata cacccacctc aacctttgaa acattcccat ttccgtggcc
     1561 gcccggccat gagccgaaag actcaccgct ggcggaagcg atcgcacagg ccgccggcca
     1621 gcttgttgaa aagcgtgacg catggctgaa tccgcccggc gcaagcgcgg atgatttgaa
     1681 aacgcgcacg ctcacgaatc tatacaacgc ccgcccggca tggcttgagg atttgcaccg
     1741 cgctttggat aatgcggtgt ttgccgctta cggttgagaa acctcaaggt actgtgacgg
     1801 cttgctccgt tgcaggtgtg taccggatgc aatcacagat ccgacgggac cagtgttcct
     1861 ctcatgacct gaagtcctct tgcgtgaggg gaaccgccgt gtcgaacagt ctcatggcat
     1921 ccgcgcgtaa ttgttggtag agtccctgcc caacgcggct cgcaaacttt aacaatggaa
     1981 gagcaccgct ctcgttaaag aaatatggtg aaacgaatgc ataacggtcc cccaacgtta
     2041 gcgagtaacc attaaacagc atgtgccctc gaatatagag ctgtccttgg gacttctcat
     2101 agttctgcag cgattcgtaa gatcgtttga tctcttcgat ctgagtcgct agagttttgc
     2161 gattttttcg aattaacgtc gccagaaatg gagacgccgg atgaagtagc acaacccgag
     2221 taaccttgtc ggggtcgcgc agacgtccat agataatatc gcggttctga tccagccagc
     2281 cccgtccgtc gttcataacc accagcagtt cccgagtcgt cttcatctca tcgagaacgc
     2341 tcgtatcctc cctgaaatta tctttcacca gcaccagtcc ggccctctgg caatccgtaa
     2401 tcacacttgg aaaatcctca aaaggcaacg cagtaagctg atggatttct tccaccagtt
     2461 gccggactcc gcgcacaaac ccccgcgaag agggaatgtg gatcgcctgt ttgtacgcta
     2521 gccgttcaag atccggaggc aactgttcgg cgctcggcat tccagcgttc cctgtcaaaa
     2581 ccggaatcac cggcacgcct cggaacagtg ccgtcgccaa ttcaatgcgg accaagtcct
     2641 tcggatcctt cagacgttct gttccgtcct tataggtcac gctcagccat tgatcgccga
     2701 tgacaccgat taccagcgag cactgagcaa gagccttatt cagctcttcc ggaaaatcag
     2761 taccggcatg aagccggtcg atgtcccgaa acgcaacttc gggaccaaaa tacatatcga
     2821 ggtgatcgtg aatacgccct gtcgcgtcct ggctgtcggt acgtcggtat gagatgaaga
     2881 tgttattcat gatttcgtcg gaccgacgcg agacgtccgt tcggagttgc cgcaaattcc
     2941 ctactgcttg aggctggatc ctatcagttt gtaagccttc tggtctaagt ttagtcgtcc
     3001 tggaaccggc cacttgaaga ctgcgcgacc tttgctcgcc ttcatcctgc cataactaat
     3061 ttcggaagac gacggaccat cccattaccc ctaggtctcc gaccgcattg agatgtacct
     3121 tagtaggcac ttggggagtg aggcaaatgg actgatagtg tagacatgca aacctaccat
     3181 cgggtgcatg tccagcttgg gaagagggac cggcagcaga ttgtcggtat gttgaacaag
     3241 ggacgggaat cggcgcgggt actgcggcgg gctttgatcc tgcgacaact caaccaggga
     3301 cagaccgcgg cgcaggtggc gggcaatgtg ggtgtggccc cgaaaaccgt gcgggcgatt
     3361 gcccggcgtt acgcggagga ggggctggat tcggcgctgt atgagaagcc gcgtccgggc
     3421 aagcagcggc ggctggaggc gggccagagc cagcggatta tcgccatggt ctgtagcgag
     3481 ccacccgcgg ggcaggcacg ctggagcgtt cggctcattg cccaggaggc ggtgcggcga
     3541 aagctggtgc ccaaggtggg gcgggagtgc atccgcatcc tcctcaagag ccacgatctg
     3601 aagccgtggc gggaaaaaaa gctggtgcgt ggcggaactg aatcaggaat acctgcggcg
     3661 catggaagag gtgctcgccc tgtatgaaaa gccgctgtcg gaaaagcaac ccgtggtctg
     3721 tatcgacgag aagccggtgg tcctgcacca ggatgtgcgg cctccgcttc ccatgcgtcc
     3781 gggacggatt gcccggcgcg attgtgaata ccagcgatgc ggcacagcca acgcgttttg
     3841 cggggtggag ccgaaggcgg gacggcactt tacgcgggcg actccggatc gctcctcgcc
     3901 ccggtttgcc gactatctgc tggcgattgc ggaatactat gcggcggccg acaccatcca
     3961 tctggtcatg gataatctga gttcgcacac ccgcaaggcg gtggtggagc ggttcggcga
     4021 gaaagcggga ggctggttat gggaccggtt cactgttcac tacacgccca agcacggaag
     4081 ctggctaaat caggcggaga ttgaaatcag cttgttctcc cgacagtgtc tgggccagcg
     4141 aagaatcccc tcgctccgcg ccctgcggcg ggaagccagg gcctggaacc gcaggatcaa
     4201 ccgccagcgg gtcaccatca actggcggtt tacgcccaca aaggcacgcg cgaagtttgg
     4261 ctacaaaaga aaccagatta cgcggtcaga gacctagtag aaatactgtg gccgcaaggg
     4321 gctctggctt ctgaaggcgg ggcctttgcg cgacgctctg cccacctgaa ggccatcgcc
     4381 ctgccactgg cactggcttt gggcgtattc gcggcgacag actggctggt ggcagggttg
     4441 aattaccggc attatggcat attcgaaacc aacgaaacca gatcgggcag ttttctgcgt
     4501 gcctacgggg ccctgtcgcg cattcagcac aaccaatggc accgttacat ttcgttccca
     4561 aaggatgttc ggctccacgc ctatgccgca agccctgcgg cccgcgaact cgaaacctcg
     4621 ctcgagggtc ccacaggaga caaatggctt caggtgacgt gctcgctatc gccggtgaag
     4681 atcactcctt gtgatgaggt ccaagcggga tggagtatgt gggagttccg cgaagcagtc
     4741 gccagcgccg gtcactatcg ctctgggcgg gaagcgatga ggttctacga cacgttggcc
     4801 aaccagatca attccgcctg cgagcgcggc accatccaat gcctgcctcg gaggtcgacg
     4861 ttgtcacctc catttcgacg cgagtacctg ggagaaactg tagaggcagg caaagttgag
     4921 gccaaggtga tgttcacgat gggcgacgga caagttggct cgatcgcgag tatcggcccg
     4981 cctgagggga ttgcgacctt cgccgacaca gtgggcggtg tggatttgcc cgacgaaagg
     5041 gtgcgggtcg tccagggttg ggctgccgcc gcatcggcga cgccaaccgt gcgcctactc
     5101 gcccacaccc cggtaccggt ccaatcctca atcagcatca cgccggcacc ggacgtgctc
     5161 gcggtttatc cggacctcaa ggcgatccgc tttgagctga caaccgattg cccggtcgct
     5221 gattgtgatt tggtgctgga catagcagga ggcgaccaac ctccggtccc cttggcgcga
     5281 ctggtccatg gagctgtgat cgatactccg cagttgcggg tttatgtgga tagtgcagcg
     5341 gtcggggagg gtacgaagct cacggactct cgtcgcgccg tccaactgaa gatcgctcgt
     5401 ctcattgcct ccgcatacgc aaaggcattt ccgatcctgg ctatcttcgg ggcaggtggt
     5461 ttgctgtttg ccaccttctt ccggcgctgg tgtccaatac ccactggtct tctagcgtta
     5521 ggtctgggta gcgctgctgc ggtcttgagc agaatcgtac tattgtccta tctcgctgca
     5581 acgtccttcc ccggcgctaa caggccgtgg tgcaagtagc ggattttctg cacggctggc
     5641 gacgtagccg ggtacgcgaa ctcacgaagc acttgacaac ggacgcggag gtccggcttc
     5701 tgcaaagccg attctgattt atccgacccc ggaaga
//
LOCUS       DASRKK010000437         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_35073, whole genome shotgun sequence.
ACCESSION   DASRKK010000437 DASRKK010000000
VERSION     DASRKK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35073"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFC15_10210"
     CDS             <1..1141
                     /locus_tag="VFC15_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM10572.1"
                     /translation="TVQQRALDLTNKTLSDNQKQVSAGTLAPLAVVQSQSAVATAKQN
                     LITAQVNLQLQQLLMKNAITRNMADPMLAVAPVIPTDTLSTTEEYEVRPVEDLIDEAL
                     KQRPEIASARLTLTNNEISRKSIRNQLLPTVDLYAFYGASALAGPQNPLCTSTSRGCP
                     PPGSIPTTGYSDAFSNLFNSSAPDKGVGININIPLRNRQIQADQVRSVLEYRQAQMSL
                     QQTENTIRLQVRQAQFAMQQNFVGLQATIAARDYAAQSLDAEQKKLRMGASTSTLVLQ
                     ASSNLTQAESNVLIAATNYEKSKVQLDLSTAETLTKLGIDMSDAETAQVKHSPKVPGT
                     VPANTNDLTQPKLTPIQVPGVTPAPQPGTPRQTPNEAQPPQPPQR"
     gene            1416..2570
                     /locus_tag="VFC15_10215"
     CDS             1416..2570
                     /locus_tag="VFC15_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10573.1"
                     /translation="MLGLDSSTDAPYDGTGFADVFVPSREAPSNRGLSYLGKHEMTRM
                     NHFTSVALVMLSAVVAASQSNREPQWIPPLVEGGTPDCPRNTGLRETRSAVAVSAHGK
                     TSAYIAASARCTIDHSCRRDATLILTESGKHQEIKLQSVAQTKTEAQKNDLDAPNPMR
                     SYSVIDFSPDDRLILLQRVGTDSWRNDTYRDVDIAVLDATATGQPKWINTWDLMHWTD
                     CHATVETQGFDKTGQPVLRVRPSVWHQHEKHDCVAYPELWAVDLRRNSATQLPDETPL
                     SRAGKTTGADWVTCENDPDIVAACFTVYGRLSVYNGGPSLRIWRVGTSRILGVTTETA
                     PENVSRLWKSDPFNTEIYGDFEVCPFTKDKAGEMQMVCIESASRLLAKQR"
BASE COUNT          626 a          832 c          760 g          543 t
ORIGIN      
        1 aaccgtgcag cagcgcgccc tggatctcac caacaagacc ctttccgaca atcagaagca
       61 ggtgtccgcc gggacgctgg ctccacttgc cgtggtgcag tcgcagagtg ccgtggccac
      121 ggccaagcaa aacctgatca ccgcgcaggt gaatctccag ttgcaacaac tgctcatgaa
      181 gaacgcgatc actcgcaaca tggcagaccc catgctcgca gtcgcgcccg tgattccgac
      241 cgacacgctg agtaccaccg aggagtacga ggtgcggccg gtcgaagacc tgatcgacga
      301 agccttgaag cagcgtcccg agatcgccag cgcgcgcctt actctcacca acaacgagat
      361 atcccggaag tcaattcgca accagttgct gcccacggtc gacttatacg cgttctacgg
      421 cgcctccgcg ctggcgggac cgcagaaccc tctgtgtacc agtaccagtc ggggctgccc
      481 gcctccagga tcgattccca ccactggcta cagcgatgcg ttcagcaatc tgttcaacag
      541 cagcgccccg gataaagggg taggtattaa catcaacatt ccactgcgta atcgtcagat
      601 ccaggctgat caggtgcgct cggtattgga atatcgccag gcgcagatga gtttgcagca
      661 aaccgaaaac accatccggc tgcaagtgcg gcaggcgcag ttcgccatgc aacagaactt
      721 tgtgggactg caggcgacca tagcggcgcg cgactacgcc gcacagagcc tggatgccga
      781 gcagaaaaaa ctgcgcatgg gcgcttccac cagcacgctc gtattgcagg cgtcctctaa
      841 cttgactcag gcggagtcga atgtcctcat tgccgccacc aactacgaga agtcgaaggt
      901 gcagctcgat ttatccacgg ccgagacttt gaccaagctc ggcattgaca tgtcagacgc
      961 ggaaaccgcc caggtgaaac attcgccaaa ggtgcccggc actgtgccag cgaataccaa
     1021 cgacctgaca caacccaagt tgacacccat ccaagttccg ggagtgacgc ccgcccctca
     1081 gccgggtacg ccacggcaaa ctccgaacga ggcccagccg ccgcagcccc ctcaacggta
     1141 gtctcttccg cccgccgagt ggtggcgttt cgttcggcgg gtggcaaggg gtctcgctct
     1201 ctaggattct cattttggcc gagtcccaca gtgacgtgat gatagtcaca tacgtaatcg
     1261 cgctgcgagg gtagcttcat tcatgcgtaa acgtgtttgg ggaaggtgct ttcagtgggg
     1321 gaactgaaca cgttctgcga aacgctggaa ggcttgacag ctcgaccagt tcgagtccgg
     1381 ctccttcctt tgggcctcca acgtgttcat gcattttgtt aggtctcgac agcagtacag
     1441 atgcgcccta cgatggaact ggtttcgcag acgtgtttgt gccgtccaga gaggctccga
     1501 gcaatcgggg cctctcgtat cttgggaagc atgaaatgac tcgaatgaac cactttactt
     1561 ccgttgcact cgtgatgctg tctgccgtcg tggccgcctc ccaaagcaac cgcgaaccac
     1621 agtggatccc gccgctggtc gagggtggca cgcctgactg tccgcgcaac accggactgc
     1681 gggaaactcg cagcgcagta gcggtatctg cccatggaaa aaccagcgcc tacattgccg
     1741 catcagcccg atgcacgatt gatcactctt gccgccgcga tgcgaccttg atcctcaccg
     1801 agtcgggcaa gcaccaggag attaagctac aaagcgttgc gcaaacgaaa actgaagcgc
     1861 agaaaaatga tctcgatgct cccaacccga tgcgctccta ctccgtgatc gatttttctc
     1921 cggacgaccg gctaatcctc ttgcagcgcg tcggcacgga cagctggcga aatgacacat
     1981 accgcgacgt tgatattgcc gtgttggatg caaccgctac cggacaaccg aaatggatca
     2041 atacttggga tctcatgcat tggaccgatt gccatgcaac cgttgaaact cagggctttg
     2101 acaagaccgg gcagccggtc ctccgcgtac gtccgtccgt ctggcaccag cacgagaagc
     2161 atgattgcgt tgcgtaccct gagctctggg cggtcgacct aaggcgaaat agtgcgactc
     2221 aactgcccga tgaaacccct ttgtcgcgtg ccggcaaaac aacgggagcg gattgggtca
     2281 catgcgagaa cgaccctgat attgttgccg cctgttttac ggtctatgga agactgtcgg
     2341 tctacaacgg cggtccatcc ttgcggattt ggcgggtcgg aacatcccgc atactgggtg
     2401 tgacgactga gacggctccg gaaaatgtgt caaggctctg gaagtccgat ccgttcaaca
     2461 ccgaaatcta cggtgacttt gaagtatgcc ccttcacgaa ggacaaggct ggtgaaatgc
     2521 aaatggtgtg tattgaatcc gcttcgcgcc tacttgcaaa gcagcgctga ataaagacga
     2581 cgttggcaag tctcccaaag cttcttgccc cccttcctcg catctactag catgtagggt
     2641 tcgcgttcaa gtggagtagc tcatgtccga tagccgcaag cctgacggga aagttctggc
     2701 cgaatcgccg gtcaccgatt tgttcgagaa tggccgccat ccctccgagt cggagaagca
     2761 g
//
LOCUS       DASRKK010000438         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_719448, whole genome shotgun sequence.
ACCESSION   DASRKK010000438 DASRKK010000000
VERSION     DASRKK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719448"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..615)
                     /locus_tag="VFC15_10220"
     CDS             complement(142..615)
                     /locus_tag="VFC15_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10574.1"
                     /translation="MDAPSKLRAWIKTNIIGDSSTSDFRAPQSPKPQSKEEQLQAEMD
                     DLKFMPPDPFPAEGGINKKLVEFWVEKLLDLALSGIGALFVWIVLKVTGKRLFPEQPH
                     GAEAIETIGPAIIWVVCYVRLCILRARQKSFYGVANMPDGCIMVPLSRSKRKFRI"
     gene            complement(702..1439)
                     /locus_tag="VFC15_10225"
     CDS             complement(702..1439)
                     /locus_tag="VFC15_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10575.1"
                     /translation="MKIGLSLLLPFLASAVMLSQTPEAPRSSSNNSGEGTASAASPPQ
                     TSILTELEKLEAAASQVSLDIGRLRVDKWKTGSIAKSAAQTNADSVQRNLTSALPGLI
                     GAVRSAPEDLNAEFKLYRNLNALCDVVSSLTDATRAFGPNSDYEALAQQSQVLDSIRR
                     KLGDSLEQQTVTTQQELNHLRVQTKAQQEQLAAAAAAPPKQIVVAQTEVPKKPAPKKK
                     TVAKKPATSGSAHSPGQPPAGVTAPKS"
     gene            complement(2427..4157)
                     /locus_tag="VFC15_10230"
     CDS             complement(2427..4157)
                     /locus_tag="VFC15_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZM10576.1"
                     /translation="MSTQTTPPSGPDHPKAGHRTATHGSWPPLFSGIRPLNRAGAARD
                     AMAGFQLAAMNIPQALGYTKIAGTPLVTGFYTLLLPLLAFAVFGSSRYLVVAADSATA
                     AILAGGLAGMAPLASARYLALAGMVALLTAGFLLLARILKLGFLADFLSQTVLVGFLA
                     GVGLQVGIAVLGEMVGVEVHSRRTVVQLLEVFRSLPQIHLPTVVISALVVAGVFALSR
                     FAPKVPGPLIAVAGAIAASAAWDFAGHGITIVGPVVGGLPHLGLPDVRWKEVMPLVGV
                     AGSCFVMIVAQSAATARFYAVRHHQQLDEDADLVGLSAANTVAALSGTFVVDGSPTQT
                     AMVESSGGQSQIAQVATATIVALVLLFLTKPVQYLPRCVLGAIVFFIAIRLIDVRALR
                     DIQRESPGEYGLALLTAVIVVLVGVEQGILLAMALSLLRVVRHSYRPHTGVFVGAEEE
                     AWHLVPALPGAVTQPGLVVYRFGAALFYANASLFAEEVRGLVGPVSSPVRWLVVDAEA
                     ITNLDYTAAQMVRQLHQELVPRGFVLALARVSPSLKADLKRHRLMEVIGPAQIFDRLH
                     DALAAFTELR"
     gene            complement(4220..>4419)
                     /locus_tag="VFC15_10235"
     CDS             complement(4220..>4419)
                     /locus_tag="VFC15_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10577.1"
                     /translation="KEKTRTQTARASFFQRRGMWKEEKKRVQELRMAKAAEIEELRRK
                     EREEEKLGPGMPPAGSEGKKQ"
BASE COUNT          885 a         1336 c         1260 g          938 t
ORIGIN      
        1 gcctcaacta ctactggaca ataaaaatgc acgaccacga caaaagggga catttctaat
       61 gtcttaaaca aggggacatt tctaatgtcc gttgacaaac aaatacaacc ggactttccg
      121 ttttgctccc aatgtccgac actatatccg gaatttcctt tttgatctcg acaacggcac
      181 cataatacac ccgtctggca tgttcgcgac accgtaaaac gatttttgcc gcgctcgcag
      241 aatacagagc cggacatagc acaccaccca aatgatggca ggcccgatcg tttctatcgc
      301 ttcggccccg tgcggctgtt ctgggaagag acgctttccg gtaaccttaa gcacgatcca
      361 aacgaacagc gcccctattc cactcagtgc caggtcgagc agcttttcca cccaaaactc
      421 gacaagtttc ttgtttatgc ccccttcggc gggaaaaggg tccggaggca tgaacttcaa
      481 gtcgtccatt tcggcctgga gttgctcctc tttcgactga ggtttcggtg actgcggcgc
      541 gcggaaatcg ctagtgctgc tatccccaat aatgttggtc ttgatccacg cgcgaagctt
      601 gctcggagcg tccaccgagt catgatctca cggcgggccc accccgcgct tatagggttt
      661 ccaattagta agcgtgacgg tgctcgacta agaaggacct cttacgactt gggtgcagta
      721 acgcctgctg gaggttgacc aggagagtgc gcactgccgg acgtagcggg tttcttggcc
      781 acggttttct tctttggagc aggtttcttc ggcacctcgg tctgggccac aacgatttgc
      841 tttggcggtg ccgccgctgc cgctgccaat tgctcctgct gggccttggt ctggactcgc
      901 agatggttga gttcttgttg tgtcgtcacg gtttgctgtt ccagcgagtc tcccaactta
      961 cgtcgaatgg aatcgagcac ctgcgattgc tgcgccagtg cctcgtagtc actgttcggt
     1021 ccgaaagcgc gggtggcatc tgtcagcgaa ctgacgacgt cgcatagcgc atttagattg
     1081 cgatagagct tgaactccgc gttcaagtcc tcaggtgcag atcgaactgc gccgatcaat
     1141 cccggcagcg ccgaggtgag gttgcgctgc acggaatcgg cgttcgtttg tgccgcgctc
     1201 tttgcgatgc tccccgtctt ccacttgtca acgcgcaatc tcccaatatc gagggaaacc
     1261 tgcgaggcgg cggcttccag cttctccaat tcagtcagga ttgaggtctg tggcgggctg
     1321 gctgcgctcg cagtgccttc accgctgttg ttggagcttg aacgcggggc ctccggcgtt
     1381 tgcgacaaca tcactgcaga ggcaagaaaa ggcagcagta gcgacaaacc gattttcatt
     1441 ttccattccg cggcgggagt tgtagacgag agtcgcggac gaggtgggcc aaacgttcac
     1501 tgatacttat agccacttcg atgccgttcg acaacacaaa gttccgtccg ggcgagcggt
     1561 gccgagtggc ttcttatttt gctaccgcac tggtgaaccc ctgtgcctcg agccagtagt
     1621 agaattcctc gagccagtgg tcgctgcggt gtcttgcttt tcatcccgaa gccgtggtcg
     1681 ccggcgctga tcgaatctga ggtaatggcg ctcggtggtc ttagttctct attgcgccag
     1741 tgtgtcagca agcccgtcag gcggagcgcg gaaaggcttg gaggctgcag acagggccgt
     1801 gacgcgttta cgtgcgcagc ttccggcctt cgctgctgct ttcctgctgt gggcgttctg
     1861 aggcggtgca aaaccctatt ttgagaccgt tttggtgctg ctgtgggcgt tctgtgggga
     1921 gtgttgttct agtcgtcaga gatatctgcc tgacacagtg ggacgctccc gtgcaactca
     1981 tcggtcaaag gcccctgggt ttctctgcga acccgtcacc attggattgg atcgtttggg
     2041 tacgaaccgc gggagtgaag gacagcggtt tttcgcagca aagttgctct gagtccgcgt
     2101 gtctaacttc ttagacagat atatgtcaga accgcatgaa aacggctgag cgccacgccg
     2161 gtccgcacag ccagctccca ggttccagat gctcagcccg acgtgccccc gccgattaat
     2221 aaacgaaaac tttcggatgg cgaccaccat caccatcgac aaccgcttgg gctgccaaac
     2281 catccttgct ctgtcactta acctcgtgct ctccagccgt ctgctccgcc gatcagctac
     2341 ccctacaatt gcgggccccg aggctctgta ctgatcgagg cctcgtttga tgcagattgc
     2401 ctctccgatg taacttgcga ggtctattac ctgagctctg tgaatgctgc cagcgcatcg
     2461 tgcaggcggt cgaagatctg cgctgggcct atgacctcca tcaagcggtg ccgctttaga
     2521 tctgctttca gcgagggcga cacgcgtgcc aacgccaaca cgaaaccgcg aggaaccagc
     2581 tcctggtgaa gttggcgcac catctgcgcg gccgtgtagt ccaggttcgt gatcgcctca
     2641 gcatcaacca ccagccagcg cacgggcgaa gaaactggcc caactaggcc gcgaacctcc
     2701 tccgcaaaca ggctggcatt ggcgtagaac agcgccgctc cgaagcgata gaccaccaga
     2761 ccgggttggg tgacagcacc gggcaaggcc ggcaccaaat gccatgcttc ttcctcggct
     2821 ccgacgaata ctccggtgtg aggacggtag ctatgccgca caacccggag cagggacagc
     2881 gccattgcca gcaaaatgcc ttgttcgacg ccgaccagca ccacgatgac agccgttaac
     2941 agggccagac cgtattctcc ggggctctcg cgctgaatgt cccgcaaggc gcgcacatcg
     3001 atgaggcgga tagcaatgaa gaatacaatc gcgcccagca cgcagcgcgg aaggtactgc
     3061 acaggcttgg tcagaaagag cagcacgagt gccactatcg tcgccgtggc cacctgcgcg
     3121 atttggctct gaccgccaga gctttcgacc atcgcggttt gggtgggact tccgtccaca
     3181 acgaacgtcc cgctcaaggc cgccacagtg ttggccgccg ataggccgac caggtccgca
     3241 tcctcgtcga gctgctggtg gtggcgcacc gcatagaaac gggccgttgc cgcgctttgg
     3301 gccacaatca tcacaaaaca cgaaccggct acgccaacca acggcatgac ctccttccag
     3361 cgcacgtcgg gcagacccaa atgtggaagc ccaccaacga cgggcccgac tattgtgata
     3421 ccgtgccccg cgaaatccca agctgcgctc gcggctattg cgccagcgac agcgatcagc
     3481 ggaccgggca ccttgggagc aaaccggctt aaggcaaaga cgccagccac gaccaaagcc
     3541 gagataacta ccgtgggcaa gtggatttgt ggcaggctgc gaaagacctc aagaagttgc
     3601 acgacggtcc tgcgggagtg aacttccacg ccgaccatct cgccgagcac ggcaatgccg
     3661 acctgcaatc ccactccggc gagaaatccc accaacacag tttgcgagag aaagtcagcc
     3721 aggaagccca atttcaggat tcgtgccagg agcagaaacc cagcggttag gagtgccacc
     3781 attccggcca gcgccaggta tcgagcgctg gcgagtggcg ccattcctgc aagcccgccg
     3841 gcaaggattg cggccgtggc ggagtcggca gcgaccacga ggtatcgcga ggaaccaaaa
     3901 accgcaaacg ccagcagcgg cagcagcaat gtatagaacc ccgtaaccag gggcgtgccg
     3961 gcgatcttgg tgtagcccaa ggcctgcggg atgttcatcg cggccagctg gaatccggcc
     4021 atcgcatcgc gggccgcgcc cgcgcgattc aatggccgta tgcccgagaa caggggcggc
     4081 cagctgccgt gagtcgccgt gcggtgccca gctttcgggt ggtctggtcc ggacggtggt
     4141 gtggtttgtg tgctcatggt cgctgcacga ttgtactttc cgtccggcac agacttaggc
     4201 gacgatttgg tgggtgccct tactgcttct tgccctcgga gcctgctggc ggcattcctg
     4261 gtcccagctt ctcctcctcc cgctctttcc ggcgaagttc ctcaatctct gctgctttcg
     4321 ccatgcggag ctcctgtact ctcttctttt cttccttcca cattccgcgc cgctggaaga
     4381 atgaggctcg cgcggtttgg gtcctggtct tctcctttt
//
LOCUS       DASRKK010000439         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_719458, whole genome shotgun sequence.
ACCESSION   DASRKK010000439 DASRKK010000000
VERSION     DASRKK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719458"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFC15_10240"
     CDS             complement(<1..363)
                     /locus_tag="VFC15_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZM10578.1"
                     /translation="MKRAPLASGQSHPVQEPNGPPAIAGGDPIRARDRRLIFGAPVIG
                     EAEIASVTECLRSNWIGLGGRVESFEQEFARYKGAPYAVGVSSGTAAIHLALLALGIG
                     PGDEVIAPAMTFCSTIHSI"
     gene            859..1635
                     /locus_tag="VFC15_10245"
     CDS             859..1635
                     /locus_tag="VFC15_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HZM10579.1"
                     /translation="MPFTVSHVAAVLPLRRLNLVWSALVVGSMAPDLPYIVGTTDYRS
                     FGHQFPGVIEFTLPASIFALWVFHTAIKRPAATLLPSGVQQRLRGQLGDFKFGGPARF
                     VAILFSLALGIATHLVWDAFTHPFSWPWRRWVWLQRWVKVPVVGPRPMYMVLQYASTV
                     IGLVALGIWVVLWYRNTAPVPAAGAATAKSGPRIALTMVAVATVAALIRAWLVIGMPK
                     SLDMADSFLLVFGVTGIALVFWEVLIYCVMISSHQTWTIS"
     gene            1798..>2502
                     /locus_tag="VFC15_10250"
     CDS             1798..>2502
                     /locus_tag="VFC15_10250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM10580.1"
                     /translation="MPSVSVIMNVRNGASTLREALESVMAQTFHDWELIVWDDRSTDD
                     SARIIAEYKDPRIRYFQSPEETSLGKARDSAIRQSSGEWVAFLDQDDIWLPRKLEKQM
                     ALADDTTGLIYGRTVRFYPSGLERDYDQTHEFTLLPEGDIFDQLFTDSCFIAMSSAVF
                     RRAAVEAIGGIPDAIQIVPDYWLYVAVARLYRVRAVQEVVCRYRMHSANTSRLAAVKM
                     NQEVLWLIDQWADQLDP"
BASE COUNT          477 a          745 c          726 g          554 t
ORIGIN      
        1 aatggagtga atggtggagc aaaacgtcat ggctggagcg atcacttcat cgccaggccc
       61 gattcccagt gcgagcaacg cgaggtgaat ggcagccgtg ccgctgctta ccccgacggc
      121 gtagggtgcg cctttgtagc gtgcaaactc ctgttcgaag ctctcgactc ttccgcccag
      181 gccgatccaa ttgctgcgca gacactcggt gaccgacgcg atctcggcct cgccaatcac
      241 cggcgccccg aagatcagcc tgcggtcacg cgcacggatg ggatcgccac ccgctattgc
      301 cggagggccg ttgggttctt gaactggatg cgattgtccg ctagccagcg gagcgcgctt
      361 cataagattc agtcttgaag ttccacgtgt gagttgattg taacctattc agaaatcatc
      421 gcgactatag cgattcccga gcgactgtgg cttgagtagt gcgaaccctc agggactgtg
      481 tcgcaacctc tgtcgtccct acgggactcg gtcttatttt ccactctacc cagcgctgaa
      541 gcgctgggct aagctctttc gcccctttcg gggctggttc attcgtactt ccttccgctg
      601 cggcaaaccg atgcgatgtc gcaacacagt ctctaaaaga cctgcactcc ccgcacgagc
      661 ggacacacca atcctggaat cgctctaggc ggtggcagcg ggcttgaatc aaccgtgcaa
      721 cgggggctgt cggctatact gaaaccgcgg gctgaagtac ttgttagaac gatccgcgct
      781 agcggccgcc ccttggagtt tcctgaccgg ctgtttcgga agtctgcaaa cttggccaca
      841 taaagcctgt tccactctat gcccttcact gtatcccacg tagccgcggt actgcccttg
      901 cgcagactaa acctggtgtg gtcggcgctc gtggtaggca gcatggcccc agatttaccg
      961 tacattgttg gaaccaccga ctatcggagc ttcggccacc agttcccggg tgttatcgag
     1021 ttcacgcttc cagcctccat cttcgcgctc tgggtgtttc ataccgccat caagcggccg
     1081 gccgcaacgt tattgcccag cggcgtgcag cagagattgc gcggccaact cggagacttc
     1141 aagttcggcg ggcctgcccg tttcgtggct atcttgtttt ccctggctct gggcatcgcc
     1201 acgcacctgg tctgggatgc gtttacccac ccgttctcct ggccatggcg gcgatgggtt
     1261 tggctgcaga ggtgggtcaa agtcccggtg gtcgggccaa ggccgatgta catggttctg
     1321 cagtacgcga gtacagtgat tggactggtt gcactcggga tctgggtcgt tctttggtat
     1381 cgcaacacag cgcccgtgcc cgcggctggc gcggcgaccg ccaagtccgg cccacggatt
     1441 gctctcacga tggttgcggt ggccaccgtc gccgcactca tacgtgcttg gctggtgatc
     1501 ggtatgccga agagtctgga catggcggat tccttcctgc tcgtcttcgg ggttacgggt
     1561 atcgcccttg ttttttggga agtacttatt tactgcgtga tgatttcgtc ccaccaaact
     1621 tggacgatca gctaagctcg gggtgtagcg gctgcggaaa aagtccggga cgcaaggctg
     1681 tcgtcggctg aagccgacac agaactgata aaaataaaca gcttgaacgc gggcctgaag
     1741 gcccgcacta cccgaacctc gcttttttta gcagcagttg tagcccgcga gattgtcatg
     1801 ccttccgtca gcgtcatcat gaacgttcgc aatggagctt ccaccctccg cgaagccctg
     1861 gaaagcgtga tggcgcagac cttccatgac tgggaactaa ttgtttggga tgaccgatcc
     1921 accgacgaca gtgcgcgaat catagccgag tacaaggacc caagaatccg ctacttccaa
     1981 tcgccggagg agacctcgct aggcaaggcc agggacagtg ccattcgcca gtcgagtgga
     2041 gagtgggtgg cttttctcga tcaagacgac atttggctgc cccgcaaatt ggaaaagcag
     2101 atggccctgg cggacgatac caccggcctg atctatgggc ggaccgtcag gttctatccc
     2161 agcggcttgg agcgcgatta cgaccaaacc cacgagttca cactcctgcc cgaaggcgac
     2221 atcttcgacc agctcttcac cgattcctgt ttcatcgcca tgagctcggc tgtgtttcgg
     2281 cgtgccgccg tggaagccat tggcggaata cccgacgcaa ttcagatcgt cccggattac
     2341 tggctctacg ttgcggttgc ccgcctctat cgggtgcggg ccgtgcagga agtggtctgc
     2401 cgctatcgca tgcacagcgc caatacgtcg cggctggccg cggtcaagat gaatcaggaa
     2461 gtgctctggc tgatagacca gtgggccgat cagctcgatc cg
//
LOCUS       DASRKK010000440         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_132113, whole genome shotgun sequence.
ACCESSION   DASRKK010000440 DASRKK010000000
VERSION     DASRKK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132113"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2443)
                     /locus_tag="VFC15_10255"
     CDS             complement(95..2443)
                     /locus_tag="VFC15_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10581.1"
                     /translation="MRSLVAMATIRSEAWLESIGLQELTHLSVDDLPFAAGDATAGSE
                     DELQAVVAGRPSDCDLPLTIRESRFFRNIARRHASGEAPRRTLLELEAFLNDSRGVWE
                     NSWIRFPESRLSAYALQVFQTDLKAVRNGSIGERSDAGRFRFTQNGEPWLRFPISYAM
                     KLSLADLAGTQPHMPEPMRAEASRLMWHFLSDNTSPETTSFHIVTANSKHSLGERVAR
                     EAARRFLFTSLLISWANRRFGLVESGQRALVYHAPVPSVHQEELSSCISDSFYRELFM
                     SPCLSGWSDGEAKYEYMHLCHQVLSRSQLNAVAKLREAGIIANELIVLPSLSNVSLAN
                     NGVHVSMGSRALDRRLRCSSGFLPGNEKRLGDLAIKIYEHFLPLFVGTYTAAPYRIGF
                     TQFHPERLLSFLPHEFDFTHLCLLWREWKEKTNLSAFGHPLTPYGPRWLDRMIARVFR
                     LRGDCVPDARLLTYPVAWLSTEHASALDGTQGNIQRLSSELDELGIVDQKMSFYMPLR
                     LREHERDGYSGFEARYYSLFPSYDRDMAPAVRLQQFLLGVAYKLALQGEWTHEQIPDD
                     PTSESERRQPFFFSAAGLPAFYVHRGSRNQLLRTILLNCKKTRPSRRHPGYVRISIRD
                     YRRAALAFLQQTGAELADSMGMRSTLVDLASRCDDRRQEASGRLMNSVMGGSTERAMN
                     VEAREFNRMAEDFYRDGLRREHLREAFSHLREDLATLEKSDEARHRIRHGVRVQDPAR
                     FLQDVEPRVPADDLSLQEVAALLNLLLLLTEQDAHRTAEELA"
BASE COUNT          525 a          766 c          751 g          472 t
ORIGIN      
        1 ggcgaataca gcgcgccaat cacagcgtca gcgactaaat gctcgcgtac cacatttccg
       61 gtagcgcggt cgatgtaccg gtgctccaca cgattcatgc caactcctcc gcagtacggt
      121 gcgcatcctg ttccgtcagc agcaggagca aattcagcag cgccgcaacc tcctgtagcg
      181 agaggtcgtc tgccggcacg cgtggctcga cgtcttgcag gaatcgcgcg gggtcctgca
      241 cgcgcacacc atggcgaatc cggtgccgcg cctcgtccga tttttcgagc gtcgcaaggt
      301 cttcgcgcaa atgtgagaag gcctcgcgca gatgctcacg tcgtagcccg tcccggtaga
      361 aatcctcggc catacgatta aactcgcggg cctcgacgtt catcgccctt tcggtggaac
      421 cgcccatcac actattcatc aagcgaccac tggcctcctg ccgcctgtca tcgcagcgtg
      481 acgcgagatc gacgagcgtc gatcgcatgc ccatggaatc agcaagctcg gcgcctgtct
      541 gctgcaggaa agccagcgcc gctcggcggt agtctcgaat cgaaatgcgg acgtatcccg
      601 gatgtcttcg actcggtcgt gtcttcttgc aattcagcag gatcgtgcgc aggagctgat
      661 tgcgcgagcc ccgatgcacg tagaaggctg gcagtccagc ggcggagaag aagaatggct
      721 ggcggcgctc gctctccgag gtgggatcgt ccggaatctg ctcgtgcgtc cactcgcctt
      781 gcagcgccag cttgtaggcg acgcccagga ggaattgctg cagtctcacg gccggcgcca
      841 tgtcgcgatc ataagagggg aataggctgt aatagcgcgc ttcaaagccc gagtagccat
      901 cgcgctcatg ctcgcgcaat cgcagcggca tgtagaacga catcttctgg tcgacgatgc
      961 cgagctcatc cagctccgac gacaagcgct ggatgttacc ttgcgttcca tccagggccg
     1021 acgcgtgctc ggtcgacagc cacgccacgg gataagtcag cagccgcgca tcgggaacgc
     1081 aatcgccgcg aagacgaaac accctggcaa tcatgcggtc aagccagcgc gggccgtacg
     1141 gcgtcagcgg atgtccgaaa gcgctgaggt ttgtcttttc cttccactca cgccatagca
     1201 ggcacaggtg cgtgaagtcg aattcatgcg gcaagaagga aagcaggcgc tcaggatgga
     1261 attgcgtgaa tccgatgcgg taaggagctg cggtataggt tccgacaaac agcggcagaa
     1321 aatgttcgta gatcttgatg gcgagatcgc cgaggcgttt ctcgtttcca ggcaggaaac
     1381 ctgagctaca tcgcaggcgg cgatccagag cacggctccc catactgacg tgaacaccgt
     1441 tgttcgcgag acttacgttt gacaggctcg gcagcacgat cagctcgttg gcgatgatgc
     1501 cggcctcgcg cagcttggct actgcgttga gctggctgcg gctcagcacc tgatggcaca
     1561 gatgcatgta ctcatacttc gcttcgccgt cgctccatcc cgagaggcat ggactcatga
     1621 acaactcgcg gtagaacgaa tccgagatac acgacgacag ctcttcctga tgtaccgatg
     1681 gaactggggc gtgatagacc agcgcacgct gtccactttc caccagcccg aagcgccggt
     1741 tcgcccagga aattagcagc gaggtgaaca ggaatcgccg tgccgcttcg cgcgcgactc
     1801 gctccccgag cgaatgtttc gagttcgccg tcacgatgtg aaaggaagtt gtctctggcg
     1861 aagtgttgtc gctcaggaag tgccacatca gccgagaagc ttccgcgcgc atgggctcag
     1921 gcatgtgcgg ctgcgtgccc gccaagtcgg ccagtgacaa cttcatcgcg tagctgatgg
     1981 gaaagcgcag ccacggctcg ccattctggg tgaagcgaaa cctgccggca tcggaacgtt
     2041 ccccaatgga gccattcctc accgccttga gatcggtctg gaaaacctga agcgcgtaag
     2101 cgctgagccg cgactcggga aaacggatcc aactgttctc ccaaactccg cggctgtcat
     2161 tgaggaaggc ctccagctcc agcagagtgc ggcgcggcgc ttcgccactg gcatggcggc
     2221 gcgcaatatt ccggaagaag cgcgattcgc ggatcgtcaa agggaggtcg caatcgctcg
     2281 ggcggcccgc caccacggct tgcagttcat cttcgctgcc ggcagtcgcg tcgcctgcag
     2341 caaagggaag atcatccacc gaaaggtgtg taagctcttg caggccgatg gattcgagcc
     2401 acgcttcact gcgaatcgtg gccatcgcta ccaagctgcg cacaaatgac gtccatgcta
     2461 atcgctgccc ctgcctcaat tttggtgcaa gaggttgacg ggcaagtcaa tgca
//
LOCUS       DASRKK010000441         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_229890, whole genome shotgun sequence.
ACCESSION   DASRKK010000441 DASRKK010000000
VERSION     DASRKK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229890"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFC15_10260"
     CDS             complement(<1..715)
                     /locus_tag="VFC15_10260"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10582.1"
                     /translation="MVFHLVGMNLRGEVVVRKKLSRRQLLQFTANLHVDLIGMEACGG
                     AHFLGRALREQGHEVRLMPAQYVKPYVKTNKSDFIDAEAIAKAVARPTMRFVPIKTDD
                     QLDMQSLHRVRERWVMRRTAIVNQIRGLLLERGITLRKGRTHVDAALPGILEDADSKL
                     SGALCVLLAQLKLELDQMAIRIDEADEVLNKTARENEACQRLTSIPGIGPITATALIA
                     AIGNGGAFRKGREFAAWMGV"
     gene            complement(955..1764)
                     /gene="istB"
                     /locus_tag="VFC15_10265"
     CDS             complement(955..1764)
                     /gene="istB"
                     /locus_tag="VFC15_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM10583.1"
                     /translation="MNGIEVPVINTAAHTSDTPQLLLEHHLKELRLPTILREYDKAAR
                     QCAVEQVDYQHYLLRITELELLDRERRATERRIRQAKFPVVKTMDSFDFLAIPSLNKT
                     LVLELARCEFLARRENVLLLGNSGTGKTHIALALGLAACQRGHRVRFTTTAALVSELI
                     EARDEKKLLRFQKQIASYELLIVDELGFVPLSKTGAELLFEMLSQRYERGSTLVTSNL
                     PFQEWTEVLGSERLTGALLDRLTHHVHILEMNGDSYRLKQSRRKQAPSSER"
     gene            complement(1761..3260)
                     /gene="istA"
                     /locus_tag="VFC15_10270"
     CDS             complement(1761..3260)
                     /gene="istA"
                     /locus_tag="VFC15_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM10584.1"
                     /translation="MFQVEIYGRVRRAVRVEGQSQRAVAKEFGLSRETVRKMLQYAVP
                     PGYQRQQPINRPKLGPWLGVIDAIVEDDKQRPVKQRHTAKRILERLKEEHGFTGGYTI
                     VKDYVRGEQLHTREMFVPLTHAPGEAQVDFGEALVVIAGVEQKAHYLAMDLPHSDDCF
                     VVAFPAETTEAFLEGHVRAFAYFGGVPTRILYDNTKIAVAKILGGEERKRTRSFSELQ
                     SYYLFADKFGRPAKGNDKGKVEGLVGYARRNFMVPIPRVSSWEELNTRLEAESRKRRP
                     RRLRGHAETIGERFERDHRAMLPLPAAPYEACEKIAGRVSSLSLVRYRSNDYSVPTQY
                     GHRQVWVKGYVHQVVIACGSEVIARHERSYERETVVFDPLHYLALLEQKTRALDQAAP
                     LAGWQLPECFAQLRRLLEARLKKHGSREYVQVLRLMETFAINEVTHAIEDTLKLGTIS
                     FDAVRHLLLCRIERRPPRLDMENYPHLPMAQVHTTRAADYMTLLVEVCA"
BASE COUNT          654 a         1071 c         1011 g          739 t
ORIGIN      
        1 ccacccccat ccaggccgcg aactcgcgcc ccttgcggaa ggcccctccg ttaccgatgg
       61 cggcgatgag tgcggttgcg gtaatcggcc caatgccagg aatcgacgtg agtcgttggc
      121 aggcttcatt ttcacgagcc gttttgttga gtacttcatc ggcctcgtcg atccgtattg
      181 ccatctgatc gagctccagc ttcagctgcg cgagtaaaac acacaatgcg ccagatagct
      241 tagaatcagc atcttccaga atgccgggca aagctgcatc gacatgggtc cgtcccttcc
      301 gcagagtgat gccacgttcg agcagcaagc cacgaatctg gttgacgatg gccgtgcgac
      361 gcatgaccca gcgttcacgc acccggtgca gcgactgcat atccagctgg tcatcggtct
      421 tgatcggcac gaagcgcatc gttggccttg cgacggcctt agcgattgct tcggcatcaa
      481 tgaaatcgct cttgttcgtc ttaacgtacg gcttcacata ctgagcaggc atcaagcgta
      541 cctcgtgccc ctgctcgcgc agtgctcggc caaggaaatg cgcgccgccg caagcttcca
      601 tgccaatcaa atcgacgtgc aggtttgcgg tgaactgcag cagttgtctg cgcgacaact
      661 tcttgcgcac aacaacctcg ccgcgcagat tcatgccgac aagatgaaag accatcttgc
      721 caagatcgat tccgattgtc cgtagttcca tgggatgcct cctccgtacg ttccttatac
      781 ctcaactgct agctgggtaa aggcggcgga ccatcccatt aactaggcca ctgcggcgga
      841 gtaaaagttg gccagtggat agttgagtag ttgttgtggg attgcggggt agaagggcct
      901 ctggagcgta gcggaagagg cccttctacc ccgaggcgcg ttcggggaca ggattcagcg
      961 ttcggaagat ggggcttgtt tgcggcggct ttgcttgagg cggtagctgt cgccgttcat
     1021 ctccaggatg tgtacatgat gtgtgagccg atcgagcagg gcgccggtca gccgttccga
     1081 gccgaggact tcggtccatt cctggaaggg taagttgctg gtcaccagcg ttgatccgcg
     1141 ctcgtatcgc tggctgagca tctcgaagag caactcggcg ccggtcttag acagaggtac
     1201 gaaccccagc tcatcgacga tcagaagttc atagctggca atctgcttct ggaagcggag
     1261 cagcttcttc tcgtcgcgag cctcgatcag ttcgctgacc agagcagcgg tggtagtgaa
     1321 gcgtacgcgg tggccgcgtt ggcaggcggc gaggcccaga gccaaggcga tgtgcgtctt
     1381 ccccgtaccg ctgttgccca gcagaaggac attctccctg cgggcgagga actcgcatcg
     1441 cgccagttcc agcaccaggg tcttgttcag cgaggggatg gcgaggaagt cgaagctgtc
     1501 catggtcttg acgaccggaa acttcgcttg ccggatgcgg cgttctgttg cccggcgctc
     1561 gcggtccagt aactccagtt cggtgatgcg cagcagatag tgttgatagt ccacctgctc
     1621 gacagcgcac tgccgtgccg ccttgtcgta ctcgcgcagg atggtcggca gccgcagttc
     1681 cttcaggtga tgttccagca gaagctgcgg cgtgtcggat gtatgagcgg cggtgttgat
     1741 aacaggaacc tcgatgccgt tcatgcgcac acctccacga gcagcgtcat gtagtcggcg
     1801 gcccgggtgg tgtgcacctg ggccatgggc aggtgcggat agttctccat atccagccgt
     1861 ggcggtcttc gttcgatgcg gcacaacagc agatggcgca cagcgtcgaa gctgatcgtg
     1921 cccaacttca aggtgtcttc gatggcatgg gtcacttcgt tgatggcgaa cgtctccatc
     1981 agacgaagta cctgcacata ctctcggctg ccgtgcttct tgagccgcgc ttcaagcaga
     2041 cgccgtagct gcgcgaagca ctcgggcaac tgccagccgg ccagtggcgc ggcctggtcc
     2101 agtgctctgg tcttctgttc cagcagagcc aggtagtgca gcggatcgaa gaccaccgtc
     2161 tcgcgctcat aactgcgttc gtgccgagcg atcacctcgc tgccacaggc gatcaccacc
     2221 tgatgcacgt agcccttcac ccatacctgg cgatgaccgt actgggtggg cacggagtag
     2281 tcgttcgacc gatagcgaac cagcgacagc gaactgacgc ggccggcgat cttctcgcag
     2341 gcttcgtaag gcgccgccgg cagaggcagc attgctcgat ggtcgcgctc gaagcgctcg
     2401 ccgatggtct ctgcgtgccc gcgcagccgt cgaggtcgcc gcttccggct ctcggcttcc
     2461 aaacgcgtgt tcagctcttc ccaactcgac acacgcggaa tcggaaccat gaagttccgc
     2521 cttgcatagc cgaccaggcc ttcgaccttg cccttgtcgt tgcccttggc ggggcgtccg
     2581 aacttgtcgg cgaacagata gtaactctgt agctcggaga aagaccgagt gcgtttccgc
     2641 tcctcgccgc ccaggatctt ggctacagcg atcttggtgt tgtcgtagag aatccgcgta
     2701 ggcacgccgc cgaagtatgc gaaggcccgt acatggcctt cgagaaacgc ctcggtggtc
     2761 tcggccggga atgccacgac aaagcagtcg tccgaatgag gcagatccat ggccaggtaa
     2821 tgcgccttct gctccactcc ggcaatcacc accagcgctt caccgaagtc cacctgcgct
     2881 tcacccggcg catgggtcaa gggaacgaac atctcccggg tgtgcagttg ttcgccgcgc
     2941 acatagtcct tcacgatcgt gtagccgccc gtgaatccgt gctcttcctt cagccgctcc
     3001 aagatccgct tggctgtgtg ccgctgcttc actggtctct gcttgtcatc ttccacaatg
     3061 gcgtcgatca ctcccagcca cggacccagc ttgggccgat tgatcggctg ctgtcgctgg
     3121 tagcctggcg gcaccgcata ctgcagcatc ttccgtaccg tctctcgcga aagcccaaac
     3181 tccttggcta ccgctcgctg actctggccc tcaacacgaa ccgcccgccg tacacgtcca
     3241 taaatctcca cctggaacat ccctccggtg ttccatgaac catgccatca gcgcaatagc
     3301 tcacgaaaac aagatccggc agtggcttgc ttttcctccg ccgcagagca ccgctcacgc
     3361 ggcgcactgt ggcctagttt tgcaccggcg cgcatactca tcagccgagt ctctctctcg
     3421 gttttctggg ccagaagaac tgatttttgc gaactgtcag ccgattgttg agcta
//
LOCUS       DASRKK010000442         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_229897, whole genome shotgun sequence.
ACCESSION   DASRKK010000442 DASRKK010000000
VERSION     DASRKK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229897"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFC15_10275"
     CDS             <1..243
                     /locus_tag="VFC15_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM10585.1"
                     /translation="VSETAGELKELVRPMIPKHRRIVVDLTDVNFVDSSGLGTLVGLK
                     VSATSAAYCSLEFINFSPLVKELLDITKLGQLFGAS"
     gene            complement(280..804)
                     /locus_tag="VFC15_10280"
     CDS             complement(280..804)
                     /locus_tag="VFC15_10280"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZM10586.1"
                     /translation="MLLDLGDLSVRSWRKDDLKALLHHANNPNIAANLRDQFPHPYTR
                     HDGIDFLDYSRSQEPECSFAIEFGGEVVGGVGFRLGRDIARISTEMGYWLGEEFWGRG
                     LATRAVTAASEWAFDNYKLSRVYAFVFSHNVASIRVLEKSGFEREGLLRRSAIKNGVV
                     IDQLLYAKVRELGR"
     gene            complement(925..1512)
                     /gene="frr"
                     /locus_tag="VFC15_10285"
     CDS             complement(925..1512)
                     /gene="frr"
                     /locus_tag="VFC15_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZM10587.1"
                     /translation="MAQISMAALPALKDGYVQLKTRMDKAVEDFRKEMTSTRTGRASV
                     HMLDGVMVEAYGSQMPLNQLANVSAPEPQLITVQPWDVSQIGAIEKAIRSGDLGLNPM
                     NDGKLVRVPVPALTEDRRKEMVKHLNRALEEHRTAVRNIRRDGNDQIKKAMKDKKITE
                     DEERGTMEHLQKLTDDEIKKMEEMAKGKEKEVMSI"
     gene            complement(1784..2566)
                     /gene="pyrH"
                     /locus_tag="VFC15_10290"
     CDS             complement(1784..2566)
                     /gene="pyrH"
                     /locus_tag="VFC15_10290"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZM10588.1"
                     /translation="MIRSPCYTDEYRSTGHLLTMTTPAYKRILLKISGEALAAGQGFG
                     VDNTRVHEIAAEIGEVHRLGVQIAIVVGGGNFFRGVAEQAKTMDRVSADHMGMLATVI
                     NALALQDALEKQGVWTRVMSAIEMNQVAEPFIRRRAMRHMEKGRVVVFAGGTGNPYFS
                     TDTAASLRAMEIKADAILKATKVDGIYTADPVLDKEATKFEEISYMDILRLGLRVMDA
                     TAIAMCRENKLPIVIFDLNKHGNIQRVVMGEKVGSLVHEGQG"
     gene            2671..>3751
                     /locus_tag="VFC15_10295"
     CDS             2671..>3751
                     /locus_tag="VFC15_10295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HZM10589.1"
                     /translation="MNTTHMEDIDDLLQRVFRLAQFRPHQREIISDVLAGHDVVCVMP
                     TGAGKSLCFQLPAVVLRGLTLVVSPLIALMADQVQQLRALNIPVLFLNSSQSGADQRA
                     ALAQLHRGFSGLLYVAPERFSAPSFQRLLPTLQPRLFVVDEAHCLSFWGHDFRPEYLR
                     LAAARKDLGSPITIALTATATTHVRKDIVDLLGLRTPKIHVTGFDRPNLAYKAQRFER
                     DADKDPSLLRLLSSQGGTGIIYCSTRRTVEQLAAYLEQQFPKRSVVAYHAGMNAAERK
                     ISQEKFMADRGSIVVATNAFGMGINKPDIRFVVHYNLPGTVEAYYQEAGRAGRDGEAA
                     ECVLYYSGRDIRTQKFFIDNIGENNP"
BASE COUNT          734 a         1163 c         1019 g          835 t
ORIGIN      
        1 gtcagcgaga cggcgggcga actgaaggaa ctggtcagac ccatgatccc caaacaccgc
       61 cgcattgtgg tcgacctcac cgatgtcaac tttgtggaca gttccggtct gggtacgctg
      121 gttgggctga aagtgtccgc gacttcggca gcttattgca gtttggagtt catcaacttc
      181 tcgcccctcg tcaaagagct gcttgacatt accaagttgg gccagttgtt cggggcttcg
      241 tgaaagggat tgactctaca ggtcgctgaa gaagtcgctt cagcgtccga gctcccgaac
      301 cttcgcatac aaaagctgat caatgacgac cccattctta atcgcactgc gccgcaacaa
      361 tccttcgcgc tcgaatccgg acttctccag cacccggatc gatgccacat tgtgggagaa
      421 cacgaacgcg taaacccggc ttagcttgta attgtcgaag gcccactccg acgcagcggt
      481 gaccgcgcgc gtggcgagtc cccgccccca gaattcctcg cccagccagt agcccatttc
      541 cgtgctgatg cgggcaatgt cgcgtccaag acgaaacccc acgcctccga cgacctcgcc
      601 gccgaactct atggcgaagg aacactcagg ctcttgcgag cgtgagtagt cgagaaagtc
      661 gatgccatcg tgccgcgtat aaggatgagg aaactggtcg cgaaggttgg ctgcgatatt
      721 cggattgttg gcgtggtgca acaacgcctt aagatcgtcc ttgcgccagg agcgcacgct
      781 tagatcgccc agatcgagca gcatgtcatc atgttgctgg acgcgatgcc cggatgcaag
      841 tggcgcctcg attccgtccg cgcccaaaat gtaaaacgga gcagcgctgg ctgctccgat
      901 attggcgcat gatgaaaccc gcagctaaat cgacatcact tctttctctt tccccttggc
      961 catctcttcc atcttcttga tctcatcatc cgtgagtttc tgcagatgct ccatcgtacc
     1021 gcgctcttcg tcttcggtga tcttcttgtc tttcatggcc ttcttgatct ggtcgttgcc
     1081 gtcgcggcga atattgcgca cggccgtgcg gtgttcttcc agcgctcggt tgaggtgctt
     1141 gaccatctcc tttcggcggt cttcggtaag cgccggcacc ggcacacgca ccagcttgcc
     1201 gtcattcatg ggattcaatc ccaggtcgcc actgcgaatg gccttctcga ttgcgccgat
     1261 ctggctcacg tcccagggct gcaccgtgat caactgcggc tcaggggcgc tcacattcgc
     1321 gagctggttc aacggcatct gcgagccata cgcctcgacc atcacgccat cgagcatgtg
     1381 cacagaagcc cggccggtgc gggtggaagt catctcctta cgaaagtctt ccaccgcctt
     1441 gtccatacgg gtcttgagct gaacgtaacc gtccttcaac gcgggaagcg ccgccatcga
     1501 aatttgtgcc atgtcgagtc caccttgcaa gtgtgaagat gatgtcgcaa acgcttgatt
     1561 ataaacccgc gtgtcgagtg cgaggttgcc agggatgtgt ctcgaactgg tctcatgatt
     1621 gtgccttgaa agggcacggc ttcagccgtg ccgcttccgg gctttgtaaa cattgggctt
     1681 tagcccctga ggtggttcga attccctcac ccccactgta gatgagagga cttctagtgg
     1741 tcagccgctt tggcgactcg ctggttgcgc gcgacccgga atgtcaccct tgtccttcat
     1801 gcaccagcga tccaaccttc tcgcccatca ccacgcgctg aatattgccg tgcttgttga
     1861 gatcgaagat cacgatgggc agcttgttct cgcggcacat cgcgatagcg gtagcgtcca
     1921 tcactctcaa gcccagccgc aggatatcca tgtagctgat ctcttcgaac ttggtcgctt
     1981 ccttgtccag caccggatcg gcggtgtaga ttccgtccac cttggtggcc ttcaggatgg
     2041 cgtcggcctt gatttccatg gcgcgcagcg aggccgccgt gtcggtcgaa aagtagggat
     2101 tccccgtgcc tccggcaaag accaccacgc ggcccttctc catgtggcgc atggcgcggc
     2161 gccgaataaa cggttcggcc acctggttca tctcgatcgc gctcatcacc ctcgtccaca
     2221 cgccctgctt ttccagcgcg tcctgcagcg ccagagcatt gattaccgtg gccagcattc
     2281 ccatatggtc ggcggacacg cggtccatgg tcttcgcctg ttcggccacg ccgcgaaaga
     2341 aattgccgcc tcccaccacg atggcgatct gcaccccgag gcggtggacc tcgcctatct
     2401 cggccgcgat ttcgtgcacg cgcgtattgt ccacaccaaa tccctggcca gccgccagtg
     2461 cttcgccgga aatcttcagc aggatgcgtt tgtaagcagg agtagtcatg gtcagcaggt
     2521 gtccagtgga gcggtactca tcagtgtagc aggggcttcg aatcacgacc gagcaactgc
     2581 cgtgatgaaa ccaattcgaa atagttacga tagtaatacc caccgtggca tatcgcttcg
     2641 cattttcttc gcagtgcaat acagtaaaac ttgaacacaa cgcacatgga agacatcgac
     2701 gatctgctgc agcgcgtgtt tcggctcgcc cagtttagac ctcatcagcg cgaaattatt
     2761 tcggacgttc tcgcaggcca tgacgtggtt tgcgtgatgc ccaccggcgc gggcaagagc
     2821 ctgtgctttc agcttcccgc ggtggtgctg cgcggactga cgttggtggt ctctcccctg
     2881 atcgccctga tggccgacca agtgcagcaa ctgcgcgcgc tcaacattcc ggtgctgttt
     2941 ctcaacagct cccaatcggg tgccgatcag cgggccgcgc tggcgcagtt gcatcgtggg
     3001 ttttccggac tgctctacgt cgcgccggaa cgcttttcag cgccgtcgtt tcaacgcctg
     3061 ctgcccactc tgcaacctcg gctcttcgtg gtagatgaag cccactgcct cagcttctgg
     3121 ggacacgatt tccggcccga gtaccttcgt ctcgccgccg cgcgcaagga tctgggatcg
     3181 cccatcacca tagcgctcac cgcgacggct acaacacacg tgcgcaaaga catcgtggac
     3241 ctgcttggtc tgcgtacgcc gaagattcac gtcactggat tcgatcgccc caacctggca
     3301 tacaaagcac agcgcttcga gcgcgacgcc gacaaagatc cctcgctctt gcgcttgctg
     3361 tcgtcgcagg gaggcactgg aatcatctac tgctccactc gccgcacagt cgaacaactt
     3421 gcagcctacc tggagcagca atttccaaaa cgttccgttg tcgcctatca cgcgggcatg
     3481 aacgccgcag aacgcaaaat cagccaggaa aaattcatgg ctgatcgcgg ctcgattgtt
     3541 gtggcgacga acgccttcgg catgggcatt aacaagccag acattcgctt cgtggtgcat
     3601 tacaacctgc ccgggacggt cgaggcctac taccaggagg ccggccgtgc tggtcgcgat
     3661 ggcgaagctg cggagtgcgt gctctattac agcggacgcg atattcgcac tcagaagttc
     3721 ttcatcgata acattggcga gaataatccc a
//
LOCUS       DASRKK010000443         8085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_914720, whole genome shotgun sequence.
ACCESSION   DASRKK010000443 DASRKK010000000
VERSION     DASRKK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914720"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..2910)
                     /locus_tag="VFC15_10300"
     CDS             complement(247..2910)
                     /locus_tag="VFC15_10300"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZM10590.1"
                     /translation="MDAFVFSLECPYPRCAREEWRDRFVKIKQAGFNTIDTYVFWNYH
                     EREKGQFDFSELEEFLSFAREFGFFVIARPGPYVDAEFERGGFPAYVIAQRFPVRSMH
                     PESLKTSKHWYDNVLPVIRRHQITEGGPIILMQIENEIDFTDVPEPEQREYIRFLARL
                     AWDAGIEVPLISNVSSVVRDRSDPDMARILDVCDFYPRWSFLTDNELPTNTTGLTMEE
                     KVGLSDRAVLASLRKMRKDEPNVPLSVAELGTGYYSKIGGKLSEDEEGADAAQINALT
                     KTIIEHGVTYINYYLGWGGSNRSWAAKGVTSTYDFAAPIREWGGLWDKYYNVKAIGDF
                     LQMFGKSLTRSQVMEKAASSTHPDMTVSERASGCSAFVFVRANTDAEHHFKLTFRDPV
                     DGNSVTVPQHGQLTMGPHAMKILPVQVPIAGGHLRYSTAELLAYGSCGERDFLLVYDA
                     PGTLVELSVRANQPPTIAGDAIYTSWDEAKPSASIGLLVGNKESYFLLNESLIIAVLP
                     RELALHTWVGKFKSGKTEDRMDIPFITDAYLLASTGSDATSVWAEVDYRPGDHVLTVI
                     LPAQPAQCMIDGKENRFQYDQHLHTATLTHTIPTPPAKSIEITQVSAWVERLETRSGK
                     WFSSSGRVLEDIGPVPFGYVKYRTHINFKDEPRAYLQSFTNNDKKVFINGKLVPGASK
                     PDRFLEFPTKGYFQPGDNTVEISYELFGSTEFGETARMAELNGVDSIRLGTDPNQWTM
                     VNTWDIQTFPAPMNGRQLDPAFIFSGKKATTLGTPSTNGKLVPAFTWCEAKFTLPDPG
                     QGWSIPWKLNFEADRDALFYLNGAFIGRYVVVGPQTSFYLSESSLRTKGQNNTLTVVL
                     AYADTVESIKTLRVEPYEDYAVRRTHVEIRW"
     gene            3983..6502
                     /locus_tag="VFC15_10305"
     CDS             3983..6502
                     /locus_tag="VFC15_10305"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM10591.1"
                     /translation="MYSAAPIRKLAFGALAISSFLAAGNAASAQVVTTGTPGSPDATT
                     TVDSRYLPPPPPKFQGEISLNADQSKTAWPARIVPPKGAPNILLILTDDVGFGAPSTF
                     GGVIPTPSLDRIAANGLRYTNFHSTSLCSPSRAALITGRNHHSVGFGVISEIATGFPG
                     YNSVIGRDSATIGRILLENGYKTAWEGKNHNTPVYQTSAAGPFDQWPTGMGFSHFYGF
                     MGGDADQWTPGSLFRDTTHITPFTGNPKWNLITAMADDAIQWLNELNDVNPDMPFLLY
                     YAPGGTHSPHHPTPEWVQKIGDMHLFDKGWNSVREQIFANQKRLGVIPQDAKLTPWPK
                     DLIKEWASLTPAEQKLFIRQADVYGAYLAYTDHEIGRVIDEVEREGKLDNTLIIYISG
                     DNGASAEGSPTGTFNEITAFNGVELTAEQQMPFYDAWGTSQTYPHYSIGWAWALDTPY
                     KWTKQIPSYFGGTKQGMAISWPAKITDKGGIRWQFHHFIDIAPTLLEVTGIPAPVMVD
                     GIAQKPIEGVSMAYTFDKANADAPSRHHTQYFEMLGVQGLYNDGWMLSADPIRAPWQL
                     QTAAVTDPASAFKFELFQLSKDWTQYTDVSAANPKKVQEMKDLMFGEFAKYQVLPLDA
                     SASPRFVAPRPNQAAGRKIFNYSGATVSIPDGNQPSVMNTSYTITADIDVPQGGAEGV
                     IVGEGGRFYGWAMYLVDSKPVFTYNLLDLKRTRFAGTEALAPGKHMIVYDFKYNGLGE
                     ATLAYNVLSGVGQGGTGTLKVDGKVVSTQPLEHTLPLVKALDQTFNIGSAGASPVDDN
                     DYKVPFKFTGTINKVTIALDPPVLTPADIKTLESANRSASD"
     gene            6873..7907
                     /gene="ada"
                     /locus_tag="VFC15_10310"
     CDS             6873..7907
                     /gene="ada"
                     /locus_tag="VFC15_10310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HZM10592.1"
                     /translation="MQSETQWRQVMERDARQDGRFVFAVRTTGVYCRPSCPSRRPRRD
                     SVEFFSDPKQAERAGYRACLRCKPTEISAQAQFVMRACQLIDEAEGVLTLAELSKRVG
                     VSPFHLQRLFKRATGLSPREYQSARRMQHVKHGLRKGDDVTTALYDAGFGSPSRLYEK
                     ASQQMGMTPGAYRRGGAGATIQYAIVPSPLGRLLVAATARGLCAVRFGEGASELEREL
                     RDEFSAADVRRDDAALQPYLEPLLASLRGENTTIDLPLDVRATAFQKKVWDALREIPA
                     GETRSYSEVARAIGDPKAVRAVATACASNPVALAVPCHRVVRSDGELAGYRWGIERKK
                     KLLEQERRGA"
BASE COUNT         1711 a         2348 c         2345 g         1681 t
ORIGIN      
        1 atcgctaccg ccgccgtcac ctcgcgcttg cttgccatgc tcagttgcct ccttctgttg
       61 cccgttgctt ccataccaaa cgggtaacat tttcatgagg caacagctac gcacgggtag
      121 caaaagtcat gagtcaatgc gccggaacaa gtagttgacg ccagacagag cttggtacat
      181 atcgaaaatg agacgttagc ggtgggccgg aagagttgag aagtttggct ttgccgatga
      241 atccgcttac cacctgattt ccacgtgggt tcgccgaaca gcgtagtctt catacggttc
      301 gactcgcaga gttttgatgg actctaccgt atctgcgtag gctaagacga cggtcaaggt
      361 attgttctgg cccttggttc gcagtgacga ttccgaaaga tagaacgaag tctgcgggcc
      421 caccacgaca tatcttccga tgaaagcccc attcagatag aagagcgcgt cccgatcggc
      481 ctcaaagtta agcttccatg gaattgacca gccctgaccc ggatccggca aagtgaattt
      541 cgcctcacac caggtaaatg cagggaccaa tttcccgttt gtcgaaggcg taccgagcgt
      601 cgtcgccttc ttgccgctga agatgaatgc cggatccagt tgacgtccgt tcatcggggc
      661 cggaaatgtc tggatgtccc aggtgttcac catcgtccat tgattgggat ccgtccctag
      721 ccgaattgag tcgacgccat tgagttccgc catgcgagcg gtttcgccaa actcggtgct
      781 gccgaagagc tcgtaggaga tttccaccgt attgtcaccc ggttggaagt atcccttcgt
      841 cggaaactca agaaagcgat cgggctttga ggcaccaggc accagctttc catttatgaa
      901 gaccttcttg tcgttgttgg tgaaggattg aagataggcc ctgggttcat ccttgaaatt
      961 gatgtgtgtt ctgtatttca cgtatccgaa gggaaccgga ccgatatctt caagcacgcg
     1021 gccgctgctg ctgaaccatt tgcccgatct tgtctccagg cgttcgaccc aggcactgac
     1081 ttgcgtaatt tcgattgact tggcgggcgg agttgggatg gtgtgggtta gcgtcgcggt
     1141 gtgcaaatgc tggtcatact ggaaacgatt ttcctttccg tcgatcatgc actgcgccgg
     1201 ttgtgcgggc aagatgacgg tgaggacatg gtctccgggg cggtaatcca cttccgccca
     1261 cacactggtc gcgtccgatc ccgtggacgc caacagatac gcatcagtga taaagggaat
     1321 gtccatcctg tcttcagtct ttcctgactt aaacttgccg acccaggtgt gcagagccag
     1381 ttcacggggc aatacagcga tgatcaggga ttcattgagc agaaagtagc tttctttatt
     1441 gccgaccagc aatccaatgg acgcagaggg tttggcttca tcccagcttg tgtagattgc
     1501 atctccggcg atcgtcggtg gctggtttgc ccgtactgag agttctacca gggtgcctgg
     1561 agcgtcatag acgagcagaa agtcacgctc cccacagctt ccgtatgcga gtagctcggc
     1621 agtcgagtag cgaagatgtc ccccggcaat gggaacctgc acgggaagga tcttcatcgc
     1681 atgtggtccc atggtgagtt gcccatgttg cggcacagtc acgctgtttc catcgaccgg
     1741 atcgcgaaaa gtcagcttga agtgatgctc agcatcggtg tttgctctga cgaagacgaa
     1801 tgcgctgcag ccactggctc gctcggagac ggtcatgtcc gggtgcgtgg agctggccgc
     1861 cttttccatc acctgagatc gggtgagcga ttttccgaac atctgaagaa aatcgccgat
     1921 cgccttaacg ttgtagtact tgtcccacaa cccaccccac tcgcgaatcg gcgccgcgaa
     1981 gtcgtaggtc gaagtgacgc cttttgccgc ccaggagcga tttgatcctc cccaacccag
     2041 gtaatagttg atgtaggtga caccgtgctc aatgatcgtc ttggtcaggg cgttgatctg
     2101 ggctgcgtct gctccttcct catcctcgga gagttttccc ccaatcttgc tgtaatatcc
     2161 tgttcctaac tcggcaacag agagcgggac attcggctcg tccttgcgca tcttgcgaag
     2221 actcgcgaga accgcccggt cggagagacc gactttttct tccatcgtca atcctgttgt
     2281 gttcgtgggc agctcattgt ctgtgaggaa gctccaccgg gggtagaagt cgcacacatc
     2341 gaggatccga gccatgtccg gatcagaacg gtctcgcaca acgctgctca cgttgctaat
     2401 gagcggcacc tctatgcccg cgtcccaggc gagcctggcg agaaagcgta tgtactcgcg
     2461 ctgttcgggc tcaggcacat cggtgaagtc gatttcgttt tcaatctgca tgaggatgat
     2521 cggcccaccc tcggtaatct ggtggcggcg aatgaccggc aagacattgt cataccaatg
     2581 cttcgatgtc ttcaggcttt cagggtgcat gctgcggacc gggaaacgct gcgcgatcac
     2641 ataggctgga aaaccgccgc gttcgaactc cgcatcgacg tacgggcccg gcctcgcgat
     2701 gacaaagaag ccaaactccc gggcgaaact tagaaactct tccagctcgg agaagtcgaa
     2761 ctggcccttc tcccgctcgt gatagttcca gaagacgtaa gtgtcgatcg tattgaaacc
     2821 agcctgcttg atcttgacaa agcggtcacg ccactcttcc cgggcgcaac gtggataggg
     2881 acactccaga ctgaagacaa aagcgtccac accgcgtatg gtgtagcatt ccgagtcata
     2941 ccggatgagt tctggccttg ggaatagttt cctctcgtaa agctccgagg gaccgttcgt
     3001 tccccaagat cgccatggcg agattgcaac tacagcaggt aaggctaaaa cgttcgcgag
     3061 gaatctgcgg cgcgacaccg acatctgacg cttccccaag cagggttgat agttccggtt
     3121 agtgtatcgg aagacgtccg tttaggacct cagggctggg attcgaggtg ttgtagtcgt
     3181 gtataacacc aaaattattc tttcacgctg ttgtttagaa tcagtcggtt aggctgaaac
     3241 tgttcgcatg tcattgataa atatacatgt tatacgtatc catttctgcc cgtgggagtt
     3301 agatggcatg gttttcgtca tttgttcgcc ttttggcttc ctttagcggc aatcgccgtg
     3361 tgccaccggg ccggagtttg ctccgctgga gcggtagtcc gcccgatgga acgtcccaag
     3421 tccagttccg accaagggtg cgattcgggt tttcaaagat tggttttcgt ccgccgcctg
     3481 ggcgcggaag tgggcagaga cacctctcct ctgacccaca catacgttgt aaagtgcgag
     3541 aagccgaagc gccagagaaa tcacgcggtg gaagaaacta aagtaaccag ggagttcgga
     3601 atccggaacg gcgggagtcg tcgatgcctt cggatggcgc gggccataat gcggactcgt
     3661 ctaacttgag cgctaccgtt ttgggataac acacggtttg gcccgctatc aacagctcgc
     3721 cgtgcaacca cccacattga ccgtccgacg cccggtaaga ctccttgctt catcgccaat
     3781 cggcgcctca tcttaaagga ccgacttgaa cttgattttg tgtcggtgcc gggataaagt
     3841 ctccgcaacg ttttctacaa tgtggggtac accttcgata catcggggcc aagtggcctc
     3901 gtgcgcgtga tcgcgggacc aacccaccgc attcctccag cgaatggagg ctaactctgt
     3961 aggacttgcg aggagatatc gcgtgtactc tgcagcccct attcgaaaac tagccttcgg
     4021 cgcgctcgcc atttcgagct tcttggccgc tgggaatgcg gcttccgcgc aggtcgtcac
     4081 taccggcacg ccgggttcac ccgacgccac cactacggtc gacagccgct acctcccgcc
     4141 gccgccgcca aaatttcagg gcgagatcag tctcaacgcc gaccagtcga aaactgcgtg
     4201 gccggcgcgg atcgtccccc cgaagggcgc gccgaacatt ttgctgatcc tcaccgacga
     4261 cgtcggcttt ggtgcgccgt ccaccttcgg cggcgtcatt ccaacgccct cgctggaccg
     4321 catcgctgcc aacgggctgc gctacaccaa tttccactcg acctcgctgt gctcgccgag
     4381 ccgggccgca ctgatcaccg ggcgcaacca tcactcggtc gggttcggtg tgatctccga
     4441 gattgcgacc ggcttccccg gttacaacag cgtgatcggc agggatagcg ccaccattgg
     4501 tcgcattctc ctcgagaacg gctacaaaac ggcgtgggag ggcaagaacc acaacacgcc
     4561 ggtctatcag acgagcgcgg cgggtccgtt cgaccagtgg cccaccggca tggggttctc
     4621 gcacttctac ggtttcatgg gcggcgatgc cgaccagtgg acgccgggct cgctgtttcg
     4681 ggataccacg catatcacgc ccttcaccgg gaatccgaag tggaacctga tcacggcgat
     4741 ggcggatgac gccatccaat ggttgaacga gctcaacgat gttaatccgg atatgccgtt
     4801 cctgttgtac tacgccccgg gcggcacgca ctcgccgcac catccgacgc ctgagtgggt
     4861 gcagaaaatc ggtgacatgc atctgtttga taagggctgg aactccgtcc gcgagcagat
     4921 cttcgccaac cagaagcggc tcggcgtcat cccgcaggac gccaagttaa cgccctggcc
     4981 gaaggacctg ataaaggagt gggcgtcgct cacgcctgcc gagcagaagc tcttcatccg
     5041 ccaggctgac gtatatggcg cgtatctcgc ctataccgat cacgagatcg gccgcgtgat
     5101 tgacgaagtc gagagggaag gcaagctcga caacacgctc atcatctaca tcagcggcga
     5161 caatggtgct tcggccgaag gctcgcccac cggtacattc aacgaaatca ctgcgttcaa
     5221 cggcgtcgag ttgacggccg aacagcagat gcctttttac gacgcttggg gtacttccca
     5281 gacttacccc cactattcga tcgggtgggc gtgggcattg gacacgccat acaagtggac
     5341 caagcagatc ccgtcgtact tcggcggcac gaaacagggc atggcgatct cctggccggc
     5401 gaagatcacg gacaagggcg gcatacgctg gcagttccac cattttattg acatcgcgcc
     5461 gaccctgctc gaagtgaccg gcattccggc gcccgtcatg gtcgacggta tcgcgcaaaa
     5521 accgatcgaa ggcgtgagca tggcctacac gttcgacaag gccaacgctg acgcgccatc
     5581 gcgccaccac acccagtatt tcgagatgct tggagtacag gggctctaca acgacggctg
     5641 gatgctgagc gcggatccga tccgcgcacc ctggcaactg cagacggcag cagtcaccga
     5701 tcccgccagc gccttcaagt tcgagctgtt ccaactcagt aaggactgga cgcaatatac
     5761 cgatgtgtcc gcggcaaatc caaagaaggt gcaggagatg aaggacctga tgttcggcga
     5821 atttgccaag tatcaggtgc tgccgctgga tgcttcggcg tcaccgcggt tcgtcgcgcc
     5881 gcgaccgaac caggctgcgg gccgaaagat cttcaactat tcgggtgcca ccgtcagtat
     5941 cccggacggc aaccagccga gcgttatgaa cacttcctac accatcactg ctgacattga
     6001 cgtgccacag ggcggggccg aaggcgtgat cgttggcgag ggtggacgtt tttacggatg
     6061 ggcgatgtat ctggtggaca gcaagccggt attcacgtac aacctgctcg acctcaagcg
     6121 cacccgcttt gcgggaacgg aggccctggc acccggcaag catatgatcg tatacgactt
     6181 caaatacaac ggactcggcg aggcgacgct ggcgtacaac gttctaagcg gtgtcggcca
     6241 gggcggcacg ggaaccctga aggtggacgg caaggtggtc tcaacccagc cactggagca
     6301 tacccttccc ctggtcaagg cgctcgacca gaccttcaac atcggcagcg ccggcgcatc
     6361 gccggtggat gataacgact acaaggtgcc gttcaaattc actggcacca tcaacaaggt
     6421 gaccatcgcg ctcgatccgc cggtactgac tccggccgac ataaagacgc tggagagtgc
     6481 caaccgctct gcgtcggact agcagtaacc ggtacgcagc atgcgaaatg gccgaggggc
     6541 ttgcgtccct cggcaccatc aaagcaatcc gagcccgtta gaattccttt gtatttcatc
     6601 cagtcgcgat ataagctagc gcacaccaaa gtccattgaa taatgctctt ggcaattcaa
     6661 accgctgggg gcattttcat tgaggctcct caatctgcac cgtcatgttc ccgccagcgc
     6721 gtgtgaaatc gccaactccc caataaaccc tctgcatggc cgtttggtga cgatggtcgg
     6781 cggccagcgg cagtaaaatc ccaagtacaa gaacttagtt tgcccgcaag ccccggatag
     6841 cgccgggaga tcaagagcgc tacctttgca acatgcaatc cgagacacag tggcgacaag
     6901 tgatggagcg ggacgcgcga caggacggca ggttcgtctt cgccgtgcgt accaccggag
     6961 tttactgcag gccgtcgtgc ccgagccggc gtccgcggcg ggattcggtg gagttcttct
     7021 cggacccgaa gcaggcggaa cgcgctggat atcgcgcctg cctgcgctgc aagccaacgg
     7081 aaatctccgc acaggcacag ttcgtgatgc gagcgtgcca actaatcgat gaagcggaag
     7141 gtgtgctgac gctggcggag ctgagcaagc gggttggggt cagcccattc cacttgcaac
     7201 ggttgtttaa gcgagccacc gggctcagcc cgcgcgagta tcagtcggcg cgtcgcatgc
     7261 agcacgtgaa acatggattg cgcaaaggag acgacgtgac tacagcactt tatgatgcag
     7321 gattcggttc tcccagccgt ctgtacgaga aagccagcca gcagatgggc atgacgcccg
     7381 gagcgtaccg gcgcggcggt gcgggagcga ccattcaata tgcgattgtt ccgtcgccgc
     7441 ttggacgatt gctggtggcc gcgactgcgc gcgggttatg cgcggtccgc ttcggtgagg
     7501 gcgccagtga gttggagcgc gaactgcgcg acgagttcag cgctgccgat gtccgacgcg
     7561 acgatgcggc gctgcagcct tatctggagc ccttgcttgc gagcttgcgg ggtgagaaca
     7621 ccaccattga tttgccgctc gacgtccgcg ccacggcctt ccagaagaaa gtctgggacg
     7681 cgctgcgcga gattccggca ggtgaaacac gaagctacag cgaagtggcg cgtgcgatcg
     7741 gcgatcccaa agcagtccgc gctgttgcga cggcgtgcgc gagcaatcct gtggcgctgg
     7801 ccgttccgtg ccatcgcgtg gtgcgcagcg atggggaact cgcggggtat cgctggggca
     7861 tcgagcgcaa gaagaagttg ctggagcagg aacggcgtgg ggcataaggc gggaaacggc
     7921 atcgcgtaat accgaatcgc acccaggcta tcgtcgctgg cgcggcgcga acctggggcg
     7981 ttacgaaacg ctgccgtgcc aacggcactc ccatgaattc tcccactcta cccagcactc
     8041 cgcctaccta ccccaatccg cgcaaaagcg gcgcgggtcg gggac
//
LOCUS       DASRKK010000444         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_719917, whole genome shotgun sequence.
ACCESSION   DASRKK010000444 DASRKK010000000
VERSION     DASRKK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719917"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..660
                     /locus_tag="VFC15_10315"
     CDS             226..660
                     /locus_tag="VFC15_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10593.1"
                     /translation="MTFEELDNELPNRFHDAELHSLKIDYVGGSAVLRMALDFGSPDG
                     PKREDYRIGELRVSGLYFCVIDPPDPTYRYVPHGSALNVSGDPAKPDTFPALEKLSRT
                     LPPGVSCYRFFVHEWNSFINIAAKDVQVSRVEEGAATATATA"
     gene            969..2450
                     /gene="trpE"
                     /locus_tag="VFC15_10320"
     CDS             969..2450
                     /gene="trpE"
                     /locus_tag="VFC15_10320"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZM10594.1"
                     /translation="MVIPDYKSFLQLSRDASLIPVAKTLSADLLTPVGAFLSLAAKQK
                     YAFLLESVEGGEKIGRYTFLGAQPRMVITARGSSVEIRRGSSVELHSIDVIELLRTCL
                     RQYRPATLPNLPPFTSGCVGYFAYDMVRQFERLPELAKDDVGLPDCVFTFYDRLLAFD
                     HLRHQLHIIAAADVSAESPRKAYDRALADIAAIEAKLTRGVHAHNFDWSTNDRKKTIK
                     VHNATSHDKFVASVRRAKEYIAAGDVFQVVLSQRLDFKPPAEPFQIYRALRTVNPSPY
                     MFYLKLDDMQVVGASPEMLVRVSGRKLEYRPIAGTHKRGATPAEDEQLIAQLRNDEKE
                     RAEHVMLVDLGRNDLGRVSEYGSVKVRDLMYVEKYSHVMHLVSALEGELRGDLDALDA
                     LAACFPAGTLSGAPKVRAMEIIEELEPVRRGVYGGSVLYADYAGNLDSCIAIRTMVLH
                     RDRAYVQAGAGIVADSDPESEYQECMNKASALLRACEASGARG"
     gene            2528..2884
                     /locus_tag="VFC15_10325"
     CDS             2528..2884
                     /locus_tag="VFC15_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZM10595.1"
                     /translation="MSHSYKDLIAWQKSMSLVKNVYLHIRGFPKEEMYGLAAQLRRAA
                     VSVPSNIAEGQGRLSKKEFKHFLSLARGSLLEVETQVLIAADLHYLSSDIAHDLETQT
                     HEVLRIINALMDSLVC"
     gene            2952..3527
                     /locus_tag="VFC15_10330"
     CDS             2952..3527
                     /locus_tag="VFC15_10330"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZM10596.1"
                     /translation="MVFVLDNYDSFTYNLVQYIGELGVEVTVRRNDQITVEEIDDLHP
                     DRIVISPGPCTPQDAGISIELIRHFAGRVPLLGVCLGHQAIGAAFGGKVVRAANLMHG
                     KTSSIEHDGKSIFRGIPSPMTATRYHSLIVQEKDLPDELEISATCVDRDGQRVIMGLR
                     HRKFPVEGVQFHPESVLTDHGRQIVRNFMDL"
     gene            3632..>3803
                     /locus_tag="VFC15_10335"
     CDS             3632..>3803
                     /locus_tag="VFC15_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM10597.1"
                     /translation="MANAVELRGIRKTYDSFVAVDDLSFSIRQGGVYGLLGPNGAGKT
                     SSIRMMIGITMPD"
BASE COUNT          825 a         1075 c         1094 g          809 t
ORIGIN      
        1 aatggagtag cttccggaca tatgcctgtg gcgaagaggg cttggtaaag gtcaactatc
       61 aggagtggcc ggtgaggata acgtattccg cctcgcggtc ccactcattc gaaaaccgcg
      121 aatgagtggg gcacccggca ataggggcaa caattttgac aggtggcgcg gcagcgccag
      181 cgctggcctt cgttttctag ggccacaagg acaggagtgt ttcaaatgac gttcgaggag
      241 ttagacaacg agctgccaaa taggttccac gatgcggaac tccacagttt gaagatcgac
      301 tacgtaggcg ggtctgcggt cctgcgtatg gcgttggact tcggaagtcc ggacggccca
      361 aaacgagagg attacagaat cggagagctg cgggtttctg gactgtactt ctgcgtgatt
      421 gatccgcctg atcctaccta tcggtacgtg ccgcatgggt ctgctttgaa cgtctcggga
      481 gaccctgcca agcccgatac attcccggcg cttgaaaaac tctcccggac ccttccgccc
      541 ggagtttcgt gttatcgatt cttcgtgcat gagtggaact cgttcatcaa catagctgcg
      601 aaggacgttc aggtttcccg ggtcgaagag ggggcagcga cagcaaccgc aacggcctag
      661 ccaagcagca taaggcacgt caggtgtctt tcctgactat ctctgggaca ttccgggcga
      721 gtagcttctc gaatgcctcc cgctcggcgg tgggaaagac gcgcttcgga atcacagtaa
      781 acgaccgaga gccgtagtag agcacgaaag agtgggcatc aacgagcgcg tgcgaataca
      841 tgctccactg aagctgtgaa tcgatgtgcc gggtgcccca cccaagcttc ttttgcttgg
      901 gtgggatagt ttggacgctg cggtgctccc gtataatcct tataaaccga gcaactctcg
      961 catatcccat ggtgattccc gactacaaaa gcttcttgca gctcagccgt gacgcctcgc
     1021 tcatcccggt ggcgaaaacg ttgagcgccg atttgctgac gccggtcggg gctttcttga
     1081 gcctggctgc gaagcagaaa tatgcgttcc tgctggagtc ggtcgaaggc ggcgagaaga
     1141 tcggccgcta tacttttttg ggtgcgcagc cgcgaatggt catcaccgcg cgcggcagtt
     1201 ctgtggagat ccggcgaggc tcttcggtgg aactccattc gatcgacgta atcgaattgt
     1261 tgcgcacctg cttgcggcaa tatcgtcccg cgacgctgcc caacctgccg ccattcactt
     1321 ccggctgcgt cggatacttt gcctacgaca tggttcggca attcgagcgc ctccccgaac
     1381 ttgccaagga cgatgttggc ttgccggatt gtgtgtttac tttttacgac cggctgctgg
     1441 cgttcgatca cctgcggcat caattgcaca tcatcgccgc agccgacgtg agcgctgaat
     1501 cgccgcggaa agcctacgat cgcgccctcg ccgacattgc cgccatcgag gcgaaactca
     1561 cccgcggagt acacgcgcac aattttgact ggtcaaccaa cgacagaaag aaaacgatca
     1621 aggtccacaa cgccacgtcg cacgacaagt tcgtggccag cgttcgccgc gccaaggagt
     1681 acattgccgc cggcgatgtc ttccaggtcg tgctttccca gcgcctcgac ttcaagccgc
     1741 ccgccgagcc gttccagatt tatcgcgcct tgcgcacggt gaatccttcg ccgtacatgt
     1801 tctacctgaa gctcgacgac atgcaggtcg tgggcgcgtc gcccgagatg ctggtgcgcg
     1861 tgtccggacg aaagctggaa tatcgtccta tcgctggcac tcacaagcga ggcgcaaccc
     1921 cggccgaaga cgaacaactg attgcgcagt tgcgcaacga tgaaaaagaa cgcgccgagc
     1981 acgtcatgct ggtggacctg ggacgcaacg acttgggacg cgtcagcgaa tacggcagcg
     2041 tgaaggtgcg cgacctgatg tatgtggaga agtactcgca cgtcatgcac ctggtctccg
     2101 cgctggaggg agagttacgt ggcgatctcg acgcactgga cgccttggcg gcttgcttcc
     2161 ccgcgggcac gctgagcggc gcgcctaaag ttcgcgccat ggagatcatc gaagagttgg
     2221 agccggtgcg ccgcggcgtc tatggagggt ccgttctcta tgccgattac gcgggcaatc
     2281 tggattcctg catcgccatt cgcaccatgg tcctgcatcg tgaccgcgcc tacgttcagg
     2341 cgggcgcggg catcgtcgcc gactccgatc ccgaaagcga ataccaggag tgcatgaaca
     2401 aggcgagtgc gttgctgcgc gcgtgtgagg catcaggggc taggggctag gagatagttt
     2461 cgagtttcaa gtttcaagtt tcaagtttcg agtttcggag tgcagagttt cgaggtcgag
     2521 caaaagcgtg tctcattcct acaaagatct gattgcgtgg cagaagtcga tgtcgctggt
     2581 gaaaaacgtc tatttacaca ttcgtggatt cccgaaggaa gagatgtacg gattggctgc
     2641 gcagttacgc cgcgcagcag tgtccgtgcc gagtaacata gcagaaggac aaggacgact
     2701 atcgaagaag gaattcaagc acttcctgtc gttggcccga gggtcgcttc tcgaagtcga
     2761 gacacaagtg ctgatcgctg cggacctcca ttacctgagc tccgacatcg cccacgatct
     2821 cgagactcaa acgcacgagg tgttacggat catcaatgca ttgatggatt cactggtctg
     2881 ctgagaggtg taggtgtgag agtcttcact tgaaacttga aactcgaaac tcgaaactcg
     2941 aaactgaagt tatggtcttt gtcttagata actacgattc tttcacctat aacctcgtgc
     3001 aatacatcgg ggaattagga gtagaggtaa ccgtgcgccg taatgaccag atcactgtcg
     3061 aagaaatcga cgaccttcat cccgatcgca tcgtgatctc tcccggcccg tgcacaccgc
     3121 aggacgccgg gatcagcatc gaactgatcc gccatttcgc aggacgcgta cctctgctcg
     3181 gcgtatgtct cggccatcag gcgattggcg cagccttcgg cggcaaggtc gttcgcgccg
     3241 ccaacctcat gcacggcaag accagttcca tcgaacatga tggcaaatca attttccgcg
     3301 gcattccatc gccgatgacg gccactcgct atcactcact gatcgtccag gaaaaagacc
     3361 tgcccgacga actggaaatt tcagccacct gcgtggaccg cgacggtcaa cgcgtgatca
     3421 tgggactgcg ccaccgcaag ttcccggtgg aaggcgtgca gtttcacccg gaaagcgtgc
     3481 ttaccgacca tggccggcaa attgttcgca acttcatgga cctctgaact gtctcgacag
     3541 gcaacagcgg ccgccagtat agtgccgttc agcgatcggt gcagcccatc tgcgtaactt
     3601 ttccggagtt ttcgccgtat caggaccaaa catggccaat gctgtagaac ttcgtggaat
     3661 ccgcaaaacc tacgacagct tcgtggccgt cgatgatctt tctttcagca ttcgccaagg
     3721 cggcgtctat gggctgctag gccccaatgg tgcgggcaag acttcctcca ttcgcatgat
     3781 gatcggcatc accatgcccg acg
//
LOCUS       DASRKK010000445        18669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1208363, whole genome shotgun sequence.
ACCESSION   DASRKK010000445 DASRKK010000000
VERSION     DASRKK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208363"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFC15_10340"
     CDS             complement(<1..200)
                     /locus_tag="VFC15_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10598.1"
                     /translation="MAATAETLNAVTVSRPQTSPWMIALAVMLATFMEVLDTSIAAVA
                     LPYIAGSVSATNDEATWVLTSYL"
     gene            complement(329..1258)
                     /locus_tag="VFC15_10345"
     CDS             complement(329..1258)
                     /locus_tag="VFC15_10345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZM10599.1"
                     /translation="MHKGSRTTGIALLAGYVAVYAATLYAMVRFGRFEAGDAIGVFAV
                     LGIGFSIAAWLLTIGIKPLPCRVLEPGRELATLLVYLLPLTAFIAFGFSAIHRHVTLE
                     PADSLAILAAKLAVFVVVPAWIMVVRFGYRLTELAPMSTQTSHTLVFLGMSLVLVVFQ
                     SVAGRGSRDIAEAHVSGNTLLFGMPLVFLWLVLEVGVVEEFFFRVLLQSRLSAALRSE
                     LGGIVLMSLIFGLVHAPGLYLRTSMTQEGLAAHPSFLMAVGYSIVITSVAGFFLGVLW
                     ARTRNFLVLILVHAATDLLPNTLPTLRAFHIVK"
     gene            1368..1580
                     /gene="thiS"
                     /locus_tag="VFC15_10350"
     CDS             1368..1580
                     /gene="thiS"
                     /locus_tag="VFC15_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZM10600.1"
                     /translation="MELLVNGEPKTCAEPASLTQFIQQLGMKGDRVAVELNREIVPRA
                     QWDAIQLHDGDHLEIVHFVGGGANSL"
     gene            1757..2149
                     /gene="ndhC"
                     /locus_tag="VFC15_10355"
     CDS             1757..2149
                     /gene="ndhC"
                     /locus_tag="VFC15_10355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZM10601.1"
                     /translation="MPDFYFARYLPLLIHVLLAGGLATAMVVLSWLVGHHKFNKAKMS
                     QYECGMQPVGDARQRFSVKFYLVAMLFILFDVEAVFLYPWAIIFKDLKMFGFWEMLVY
                     IGIVLAGYWYIWKKGVIDWNKQGKAERI"
     gene            2152..2649
                     /locus_tag="VFC15_10360"
     CDS             2152..2649
                     /locus_tag="VFC15_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZM10602.1"
                     /translation="MALAPAITDLEQLKDRPELARLLSWKTDAVQGAKFDRNELSIYI
                     ERTFIREACTTLKNDPELNFDSLADLTCLDWEPSDPRFEVIYHLFSTVTKKRLRLKVR
                     LSGDDARVDSLTPIWPGADFFEREVFDLFGVRFQEHPNLKRIMMPENWEGHPLRKDYP
                     VEGYR"
     gene            2653..3858
                     /gene="nuoD"
                     /locus_tag="VFC15_10365"
     CDS             2653..3858
                     /gene="nuoD"
                     /locus_tag="VFC15_10365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZM10603.1"
                     /translation="MAEMTPTPVLDSATDQTVVLNMGPQHPSTHGVLRLLLEVDGETI
                     VRIMPDIGFLHTGIEKTCEAKFYQQVVPLTDRIDYLAPMTNNLAYCLAVEKLLGLEIP
                     PRAQWLRVMLNELTRINSHLVWLGTHAMDIGAMTVFLYCFREREEILRLFEMVSGQRM
                     MTSYFRIGGVSIEPPLDFFDRVKRFITPFPERIDQYENLLTSNPIWVMRTKGVAYLSA
                     EDALALAATGPTLRASGIDVDLRRDNPYTGYENFKFNVPVSKDGDVYARYLVRVAELR
                     ESQKMVVQALEGIPDGPVKADAPKVVLPDREKMKTQMESLIYHFKIVTEGFTVPEGEV
                     YMPIESPRGEMGYYVVSDGTAKPYRVHMRSPSFANLQSLRKMCEGRMIADVVAAIGSI
                     DIVLGDCDR"
     gene            3909..4391
                     /locus_tag="VFC15_10370"
     CDS             3909..4391
                     /locus_tag="VFC15_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZM10604.1"
                     /translation="MKFSDQLEQRFAEMSTHYPTKRSVLVPMLLYIQDEVGCLTDEAI
                     HEIAHRTELSDLEVRNVVSYYSMLRTHPIGKYNLQVCTNVSCLLRGGEEILEHCKKKL
                     GVGNKQSTADGLFYLEEVECIGACSWAPAMQVNYDYHENLTNETIDELIDGIRKKEQQ
                     "
     gene            4402..5709
                     /gene="nuoF"
                     /locus_tag="VFC15_10375"
     CDS             4402..5709
                     /gene="nuoF"
                     /locus_tag="VFC15_10375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZM10605.1"
                     /translation="MAYLVSHPDEVKVVSKRFGMGATNLDRYLELDGYKALQKAIGMG
                     PDAIINEMKVSNLRGRGGAGFPTGMKWSFVPKQSAKPKYVLCNGDESEPGTCKDRLIF
                     EQDPHSVIEGVMIAALAVGSKTGYIYLRGEYRYLIQIMEKAVADAYARGFIGKNIFGS
                     GVDLDIFVHSGAGAYEVGEESALMESLEGKRGIPRIRPPFPAVVGLWGGPTVINNAET
                     LASVPHIILGGGQWFADIGTPKNGGTHLVSISGNVARPGVYEMALGYNLKKMIYEVAG
                     GIPNGRKLKGVVPGGSSVPILLPEEIDILADYDGLAKAGSMLGSGAVIVLDDSMCIVR
                     FALRVAKFYQHESCGWCIPCREGTDWLKKTLTRFHAGGGVAKDIDNLKYLAENMLGRT
                     FCPLGDAAAMPIISYVQKYRKEFEEHLDGKPCPFEKAAEPALV"
     gene            5740..8109
                     /gene="nuoG"
                     /locus_tag="VFC15_10380"
     CDS             5740..8109
                     /gene="nuoG"
                     /locus_tag="VFC15_10380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZM10606.1"
                     /translation="MPDVTLTVDGKKLTVPAGTLLIEACKTKGIEVPAFCYYPGLSLQ
                     GACRMCLVEIEKMPKLQTACTTPVAEGMIVTTESDKIKQARKSMIELLLANHPLDCPV
                     CDAGGECELQDMTFKYGAAESKFMDMKNHREEQQWSPVVYFDRPRCILCYRCIRVCGE
                     GMDVWALGIQNRGSSSIIAPNKEDHLECEECGMCIDICPVGALTSGAYRYKTRPWEMK
                     HVGTVCTHCGDGCKTTLGVRRADTGMEIVRGDNRDKSGINGDFLCIKGRYAFDFANNS
                     ERLSTPLIKKDGKFAPAKWDEAFNLIASKFKEALASSGLSSVGVIGSNRTSNEENYLL
                     QKFARLVLGTNNIDHHRTADFPAFASALAGKPTATASMRDVATAPAILLIGNNPTEQH
                     PLLAWQIRTNVRLHRAKLYVVNFSPIKLRRQATAFAQIAPGSEGGFVQFLTGNDSAAD
                     SVTSTHVTREALNELREKLKVEQGLVIIFGSELRGADVKTLVDFGLAQGARFVCLGDY
                     ANSRGASDMGLYPDLLPGYAPVDSATKFHEKWLDAIPKAAGLNLLQMADAAKDGRLKA
                     LYVVGSNPVARYNIDPFALSNAFVVVQDMFLTETAMIADVVLPVANAYEKSGTYTNTA
                     GDLQLLKKAGDFAGTRTDFECIVRIAQTMGYDVSKLVPFGNTGTRADMGQTRGAQSGE
                     SDQNAVWLTARNLESKMSPYDPVAMLDEIQRLVPGYDVSRLGVLAGQDEHTQLVQITG
                     ATGTGHPELILPSGDTLFTSGTLGRYSATLNSVMERNQTSPADKEVAAD"
     gene            8206..9240
                     /gene="nuoH"
                     /locus_tag="VFC15_10385"
     CDS             8206..9240
                     /gene="nuoH"
                     /locus_tag="VFC15_10385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZM10607.1"
                     /translation="MTIGTFILVSLIKIVIAMFVLLTGVAYTTLAERKVVAHIQNRWG
                     PSRVGPFGIWQPLADGLKFILKEDITPPFANKPLFLLAPILSLTVALTAIAMVPIGPW
                     VTIGGIHTPLQIADVNIGLLIILGLTSIGVYGVALAGWSSNSKYSMMGSLRATAQMVS
                     YEIALGLSLVGVLIYAGSFSLADIVNAQSGTFLGFIPKWNIFTGQFVAFFIYVMAAYA
                     ETNRIPFDLAEAETELVAGYHTEYSSMKFAMFFMAEYANMFTVACLATVLFLGGWSGP
                     IFGPPILQAFLPIVWFVVKVLAFIFVYIWVRGTLPRFRYDQLMAFGWKFLLPLAIANI
                     LVTSLVVALK"
     gene            9298..9816
                     /locus_tag="VFC15_10390"
     CDS             9298..9816
                     /locus_tag="VFC15_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZM10608.1"
                     /translation="MHLVLFLIFGAVCVGAAINLLVQKHPINSALSLIVVMGSLALLY
                     LLLGAEFVAAVQVIVYAGAVMVLFVFVIMLLNAGEEERTPHGSRVATLLGVPGLIVGL
                     SIGIWVVTHYAGLDRIAIGGPAILNGTEGFGNPRAVAQLLFRDYLLPFEVTSVLILIA
                     IMGAVVLARREE"
     gene            9817..10119
                     /gene="nuoK"
                     /locus_tag="VFC15_10395"
     CDS             9817..10119
                     /gene="nuoK"
                     /locus_tag="VFC15_10395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZM10609.1"
                     /translation="MVPLSYYLILSAILFACGVTGFLIKRNIITIFMSIELMLNAVNL
                     TFVAFAAELRMLSGHVFVFFVMVVAAAEAAVGLAIIISVFRTRETLNVDRVNLLKL"
     gene            10116..12119
                     /gene="nuoL"
                     /locus_tag="VFC15_10400"
     CDS             10116..12119
                     /gene="nuoL"
                     /locus_tag="VFC15_10400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZM10610.1"
                     /translation="MSLHLWIIPLLPLTGAAINGLLGKRFPKAIVNAIALGATASAFA
                     YALYVAVQFMGLPADQIPHIERYATWMTAGLFTAEYGIYLDQLSLVMLLIVTGVGFLI
                     HIYSVGYMEHEGGYYRYFAYLNIFMFFMLTLILANNYLLMFVGWEGVGLASYLLIGFW
                     FLKDSAANAGKKAFITNRVGDFGFLIALFLLIKHFGSLQYETVFTALSGYPVEASAGL
                     LTAIGLLMMVGASGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSNAV
                     FNRAPIALTVVAVIGTLTAIFAASIGIVQTDIKKVLAYSTISQLGYMFMGCGVAAYGA
                     GIFHLMTHAFFKALLFLSAGAIIHGLGGQQDMRHMGGLRKYMPKTFWCMTIATFTIAG
                     FPPLAAFFSKDEILWATWASGNHVLWLIGLIAAAMTSFYMFRLWFMTFFGEYRGATAG
                     ESQHGHDSHDAHAHGTPHESPMVMILPLMILAVLSFVGGWVGVPAALGGSDRFDHFLD
                     PVFAAHSPVETKLVAPTNEELAAHHEETGTELLFMGISVSAAALGFLGAWFLYYKRTD
                     LPAKMAAAAGGLYTFVLDKYKVDEFYGAAVIRPIVLASTNILWKGIDATLIDGAVDES
                     AVGARDISDGLRHMQSGNIRSYAGWVAFGAAVVIAFMVWKGVR"
     gene            12116..13669
                     /locus_tag="VFC15_10405"
     CDS             12116..13669
                     /locus_tag="VFC15_10405"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZM10611.1"
                     /translation="MNYIDNNIITIITFLPFAGALLLCIFPRRDRDIRWFALGISLVT
                     LLASLHLPWHYVRGLDGFQFEQNVPWISHPNIHYHLGIDGISMWLVVLTTFLTPLCVL
                     ISWKSVNSQVKEFFILMLILETAMIGVFVALDLFLFYFFWEATLIPMALIIGIWGHGR
                     RIYAAVKFFLYTMIASVFMLAAIIWLYTHTGSFDFVTIRDAIQSGQVEGFSTAAKWLF
                     LGFFVAFAVKVPLFPLHTWLPDAHVEAPTAGSVLLAGVLLKMGTYGLLRFAVGLFPDQ
                     ARSNAPWIIVLAIISIISGALVAMVQPNVKKLIAYTSVSHLGFVVLGIFSFTQIGMDG
                     AVYQMLGHGISTGALFMLLGMIYERRHTYEISEYGGIATPMPVYATFFTFIMLSSVGL
                     PLLNGFVGEFLILSGAFQARQIYGILAATGVIWSACYLLWMYQRVFYGNITHPVNKNL
                     QDMDARERVAMWPLAVAALVMGVAPNLWLHSIDPSVSFALKQFSSGVQAAAPSASHVT
                     QALMQVIGR"
     gene            13666..15102
                     /locus_tag="VFC15_10410"
     CDS             13666..15102
                     /locus_tag="VFC15_10410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZM10612.1"
                     /translation="MIPTVDYIRILPEVVLTIFGILVMLVDPVIPTQDSKKPLGVIAL
                     IGVLAGLAATLYQSRFYGDAFYHMVRVDAFSIFFHLVILLIALVVILSSYDYLEVQQI
                     KAGEYYGLVLFATVGMMLMSSAIELVLIFIALEISSISTYILAGFRRRAAESAEASLK
                     YFLLGSFATAFFLYGVAMVFGSTGSTNIYLIAEILKTHVSVLTYLAVALMFVGLGFKV
                     ASAPFHVWTPDVYEGSPAPVVGFMSTGPKAAAFAVLLRVLFATGAPGWFWLVWISAVL
                     SMSIGNIGALVQNNVKRLLAYSSIAHAGYVLVAFAAAQTMGISAAMFYAASYAAMNVG
                     AFAIVAHLSGKGEKYVTLEDYAGLGRRSPLIASILTIFLLSLIGIPITGGFFAKFYVF
                     SAAMHSNLVVLTIIGVINSAIAAYYYLRIIVYMYMRDERAVEPPVAPIPFSLGAGLLI
                     SLIAVIYLGVLPNDVLDYAIRGAQDLLK"
     gene            complement(15185..15496)
                     /locus_tag="VFC15_10415"
     CDS             complement(15185..15496)
                     /locus_tag="VFC15_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZM10613.1"
                     /translation="MRKLSIGMFSLLAIMMMTMPAYAQGGAAAAPTTNWVAIASGFGM
                     AIAAGLAALGQGKVGAAACEGMARNPGARPGIQLALILGLAFIESLVLFTLVIIFAKV
                     V"
     gene            complement(15579..16310)
                     /gene="atpB"
                     /locus_tag="VFC15_10420"
     CDS             complement(15579..16310)
                     /gene="atpB"
                     /locus_tag="VFC15_10420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZM10614.1"
                     /translation="MPEQLWVTAFLNKYLAGVTNAILGVFHFHSSHPQAPITNYVAMQ
                     LVVFVLLVLIFALVRSRLSVENPGNLQHVFESIDAMISQQGLEVIGHGYERFTNYLIT
                     LGIFILTCNLIGLIPGFEAPTAVPSVPLGCAVVTWIYYQMHGIRANGPGYIKHFLGPV
                     WWLAPLMFVIEVASHLARILSLTVRLFANMFAGDMVTLVFFSLFPLAIPVLFMLLHVG
                     VSFIQTYIFVLLATVYIGEAVAHEH"
     gene            complement(16409..16843)
                     /locus_tag="VFC15_10425"
     CDS             complement(16409..16843)
                     /locus_tag="VFC15_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZM10615.1"
                     /translation="MSATEPSARDSPAEKFYGAALGRIRRFIVILGGAGVLVCLLSFG
                     RVVAAGFLVGAVISYVNHVWLERMIEALGERITSGESRERGGILVVRAVLRYLFIALG
                     AYVIFRVSLAGLYGFLGGVCLTIAAIACEAVVEVYVGLRRGF"
     gene            complement(16894..17685)
                     /locus_tag="VFC15_10430"
     CDS             complement(16894..17685)
                     /locus_tag="VFC15_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10616.1"
                     /translation="MYRIAAWIRLILRGFLLLLIAALTVSCFSQEILSTYADLSISGQ
                     WQSGKKYATVETGSDIFYDPGTGAVIEISQQAGLHKAGEIAKFFTGINASSKDAAGVM
                     SAGAFPLPFLYAEKASKDLAKGTKPPRISDMKDGEGNPLWFYVSQLFDAYQMHDSGGS
                     SEVREDFLPVRVTKAEQRSVPGGDALLFEVENEKPISETVIKHFHMPAAYKDQRMRFG
                     WVQFAPGGIASGQGVLSVAFATAANSTLNIEEVVKQVSAAKIKPL"
     gene            complement(17813..18040)
                     /locus_tag="VFC15_10435"
     CDS             complement(17813..18040)
                     /locus_tag="VFC15_10435"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZM10617.1"
                     /translation="MSDERSNKGSDNPAVIWAKYSEIAFIIPAAVVVGLLIGKLLDYW
                     LHTRWIFLGGIIVGAVVGFAQMIVMVTRAKD"
     gene            18321..18397
                     /locus_tag="VFC15_10440"
     tRNA            18321..18397
                     /locus_tag="VFC15_10440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18355..18357,aa:Met,seq:cat)
BASE COUNT         3865 a         5447 c         5229 g         4128 t
ORIGIN      
        1 aggtagctgg taagcaccca ggtggcctcg tcattcgtgg ccgatacgct gcccgcgata
       61 tagggcagcg ccaccgccgc gatggaagtg tcgagcacct ccatgaacgt cgccagcatg
      121 accgccaggg cgatcatcca cgggctggtt tgcggacggg agacagtcac cgcgttgagc
      181 gtttctgcag tcgctgccaa agtccctctg tattaacgaa gaatgtaatt tgtgagcgcc
      241 gttttggcta ctgtggccag cttccccaag ctggaatatt gatcggatgc gcaagagcgc
      301 atttggaagc gagaaattcc gcggtggttt acttcacgat atggaaagca cgcagtgtgg
      361 gtagcgtatt tggtagcagg tcggtggcgg catggaccag aatgagtaca agaaagttgc
      421 gcgttcgcgc ccacaatacg cccaggaaga acccggcaac ggacgtgatc acaattgaat
      481 atccgactgc catcaagaaa gacggatgcg ccgccagccc ttcctgcgtc atgctggtgc
      541 gcaagtagag tcccggggcg tgcaccagtc cgaagatcag cgacatcagg acgatgccac
      601 ccagttccga acgcagtgct gccgacagac gcgactgcaa cagcacgcga aagaaaaact
      661 cctccaccac gccgacctcg agcaccagcc agaggaatac gaggggcatc ccgaacagca
      721 gtgtattgcc ggagacgtgg gcctcggcga tgtctcgcga cccccgcccc gcgaccgact
      781 ggaagaccac caagaccagc gacattccca ggaacacaag cgtgtggctg gtttgcgtcg
      841 acatcggcgc cagctcagtc aatcggtagc caaatcgaac caccatgatc cacgccggaa
      901 ctaccacgaa gactgccagt tttgccgcaa gtattgccag cgagtcagcc ggttccaaag
      961 tgacgtggcg gtggatcgct gagaaaccga acgcgataaa ggcggtcagc ggcagcaaat
     1021 atactaatag agtcgccagt tccctccccg gctctagaac gcgacagggt agcggcttga
     1081 tcccgatcgt cagcagccat gcggcgatcg aaaaaccgat tcccaggaca gcgaatacgc
     1141 cgatagcgtc gcccgcctcg aagcggccga agcggaccat ggcatagagc gtcgccgcat
     1201 agaccgcaac atatcctgct agcagcgcta ttcccgtcgt cctgctgcct ttatgcaacc
     1261 gtcggttctc caaaacgtgg gtcgccatgt tgactggtta cgcgcccgct gcacgatttg
     1321 ttccccgggc cgtgaaatcc catcgcacat tgggatacat ttcttgcatg gaattattgg
     1381 tgaatggcga accgaaaacc tgtgccgaac cagcctcgct gactcagttc atccaacaac
     1441 ttgggatgaa gggagaccgt gtagccgtcg aactgaaccg cgaaatcgtg ccgcgtgctc
     1501 aatgggacgc gatccaatta cacgatggcg accatctgga gatcgtccat tttgtcggcg
     1561 gcggtgcaaa ttctctctga atttgccgtg ttttgttttc gcggaacgca acttctcagc
     1621 gtcttctctg ctgctttgtg aaacccgcgt gaattgctca ccacgattgg ttgacatgag
     1681 cgctaacgca aaataaactc aggaagttac acgtgtttcc tggtagtgcg gcaactctta
     1741 agtttcacct tcaatcatgc cggattttta tttcgcacga tatctgccgc tgctgatcca
     1801 cgtccttctg gccggtggac tggccacggc catggtggtt ctttcgtggt tggtcggcca
     1861 ccacaagttc aataaagcga aaatgtcgca gtacgagtgc ggcatgcagc ccgtgggcga
     1921 tgcgcgtcag cgcttctcgg tcaagttcta tctggtcgcc atgctgttca tcctgttcga
     1981 cgtcgaagcc gttttcctct atccctgggc gatcattttc aaggacctga agatgtttgg
     2041 tttctgggag atgctggtgt acatcggcat cgtgctcgca gggtactggt acatctggaa
     2101 aaaaggcgtg atcgactgga acaagcaggg caaggcggag aggatctgag catggctctg
     2161 gcaccggcaa tcaccgacct tgaacagtta aaagaccggc ctgaactggc tcgcctactt
     2221 tcctggaaga ccgacgccgt ccaaggtgct aagttcgatc gcaacgagtt gtccatctac
     2281 atcgaacgca cttttattcg tgaagcctgt acgacgctca agaacgatcc ggaactcaac
     2341 ttcgactcgc tggccgacct cacctgccta gattgggagc ccagcgatcc ccgcttcgaa
     2401 gtcatctatc acctgttttc gactgtgacc aagaaacgtc tccggctgaa agtgcggctt
     2461 agcggcgacg atgcccgagt ggattccctc actccgatct ggcctggggc ggatttcttt
     2521 gagcgcgaag tcttcgacct gtttggcgtc cgcttccagg agcaccccaa cctgaagcgc
     2581 atcatgatgc cggaaaattg ggagggacat ccgttgcgca aggattaccc cgtcgaggga
     2641 taccggtaaa tcatggcaga aatgacacca actccggtcc tggattcggc gaccgaccag
     2701 acagtggtac tcaacatggg tccgcagcac ccctcgaccc atggagtgtt gcgcctgttg
     2761 ctcgaggttg atggcgaaac cattgtgcgc atcatgcccg atatcggctt cctgcacacc
     2821 ggcatcgaga agacctgcga agccaagttt taccagcagg tcgtgcccct gaccgaccgc
     2881 atcgactatc ttgccccgat gaccaacaat cttgcgtact gtctcgcggt cgagaagttg
     2941 ctggggctgg agattcctcc gcgcgcgcaa tggctgcggg tgatgctgaa tgagttgacg
     3001 cgcatcaatt cgcacctcgt ctggctggga acccacgcaa tggacatcgg tgcgatgacg
     3061 gtgtttctgt attgcttccg cgagcgtgaa gagatcttgc ggctgtttga aatggtttcc
     3121 ggacagcgca tgatgacttc gtatttccgc attggcggag tctcgattga accgcctctc
     3181 gacttcttcg accgggtgaa gcgcttcatc acgccattcc ccgagcgcat cgatcagtac
     3241 gagaacctgc tgacttcgaa cccgatctgg gtgatgcgca ccaagggcgt cgcgtatctc
     3301 agtgccgaag atgccctggc gcttgctgcc accggtccta cgctgcgagc cagcggaatc
     3361 gacgtcgatc tgcggcgcga caatccctac accggatacg agaacttcaa attcaatgtg
     3421 ccggtctcga aagacggcga tgtttacgcc cggtacctgg tgcgggttgc ggagttgcgc
     3481 gagtcgcaga agatggttgt gcaggcgctc gaaggcattc ccgacggacc ggtgaaggcg
     3541 gatgcaccca aggtcgttct tcccgatcgc gagaagatga agacgcaaat ggaatccctc
     3601 atctatcact ttaagattgt gacagaaggt ttcacggtgc cggaaggcga agtttatatg
     3661 ccgatcgagt ctccgcgagg cgagatgggc tactacgtag tgagcgacgg aacggcgaag
     3721 ccgtatcgtg tgcacatgcg gtcgccatcg tttgccaacc tgcaatcgct gcggaagatg
     3781 tgcgagggac gcatgatcgc cgacgttgtg gcagcaatcg gcagtatcga tatcgtgttg
     3841 ggcgattgcg atcggtagaa cagtggccgg tggctagtgg ctggtgacta gttttgtgag
     3901 cgagcggaat gaaattctct gaccaactgg aacagcgatt cgcggagatg tcgacgcact
     3961 atccgaccaa gcgctcggtg ctggtgccga tgctgcttta catccaggat gaagtcggct
     4021 gcctgacgga tgaggccatt cacgaaatcg cgcatcgcac cgagttatcg gacctggagg
     4081 tgcgcaacgt tgtcagctat tactcgatgc tgcgcaccca tcccatcggc aagtacaacc
     4141 tgcaggtctg caccaacgtg agttgcctgc tgcgtggcgg cgaggaaatt ctcgagcact
     4201 gcaagaaaaa actcggcgtt ggcaacaagc agtccacggc tgacggtctc ttctatctcg
     4261 aagaggtcga gtgtattggc gcctgctcct gggccccggc gatgcaagtg aattacgact
     4321 atcacgagaa cctcaccaac gaaacgattg atgagttgat cgacggcatc aggaagaaag
     4381 aacaacagta acaaagcaac catggcttac ctggtctcac atcccgacga agtgaaggtt
     4441 gtctccaagc gttttggtat gggagcaacc aacctcgacc gctacctaga gctcgacggt
     4501 tacaaggcgt tgcagaaagc catcggaatg ggcccggacg cgatcatcaa tgagatgaag
     4561 gtctcgaact tgcgcggacg tgggggcgcc ggcttcccta ctggcatgaa gtggtcgttc
     4621 gttcccaagc agtcggcaaa acccaagtac gtattgtgca acggtgacga aagcgaaccg
     4681 ggcacatgca aagaccggct tatcttcgag caagatcctc actccgtgat cgaaggcgtg
     4741 atgattgcgg cactcgccgt gggttccaag accggctaca tctacctgcg cggcgagtat
     4801 cgctacctca tccagatcat ggagaaggcg gtcgccgatg cttacgcccg gggcttcatc
     4861 gggaagaaca tcttcggctc gggcgtcgat ctcgacatct ttgtgcattc cggcgcaggg
     4921 gcctacgaag tcggtgaaga gtcggctttg atggagtcgc ttgaaggcaa gcgcggtatc
     4981 ccgcgcatcc gccctccttt ccccgcagtt gtgggactct ggggcggccc cacggtgatc
     5041 aataacgctg agactctcgc tagcgtcccg cacattattc tgggcggcgg gcaatggttt
     5101 gccgatatcg gcactccgaa gaacggcggc acacacctgg tcagcatcag tggcaacgtc
     5161 gcaagacccg gcgtgtatga aatggccctg ggctacaacc tgaagaagat gatttacgag
     5221 gtggccggcg gcatcccgaa cggacgcaaa cttaagggag tggtacccgg cggatcttcc
     5281 gtacccatct tattgccgga agagatcgac atccttgcgg actacgatgg gctggcgaag
     5341 gcgggctcca tgctgggatc gggcgctgtc atcgtgctcg acgactcgat gtgcatcgtg
     5401 cgcttcgcgt tacgcgtggc aaagttctac caacacgaaa gctgcggatg gtgcatcccc
     5461 tgccgcgaag gtacggactg gctgaagaag accctgacgc gcttccatgc gggcggcggc
     5521 gtggccaagg acattgacaa cttgaagtac ctggcagaaa acatgctggg ccggacgttt
     5581 tgtccgctgg gcgatgcagc ggcgatgccc atcatttctt acgtgcagaa gtatcgcaaa
     5641 gagtttgagg aacacctcga cggcaagccg tgtccatttg agaaggcggc cgaaccggcg
     5701 ctggtttgat gcgcggattt tgtttgatca aggacaattg tgccagacgt aacactcact
     5761 gttgacggca agaagctgac ggttccagca ggaacgctgc tcatcgaagc ctgcaagacc
     5821 aagggtatcg aggtgccagc attctgctac taccccggcc tttcgttgca gggcgcgtgc
     5881 cgcatgtgcc tggtggaaat cgagaaaatg cccaagctgc agaccgcctg caccacgccg
     5941 gtcgcggaag gcatgatcgt caccaccgag agcgacaaga tcaagcaggc gcgcaaatcg
     6001 atgatcgagc tgttgcttgc caaccatcca ctcgattgcc cggtatgcga cgcgggcggc
     6061 gagtgcgaac tgcaggacat gaccttcaag tacggcgcag ccgaatcgaa gttcatggac
     6121 atgaagaacc accgcgagga gcagcaatgg tctcccgtgg tgtactttga ccgcccgcgc
     6181 tgcatccttt gttaccgctg catccgcgtg tgcggcgagg gtatggacgt ctgggccctg
     6241 ggaattcaga atcgcggatc gagttccatc attgcgccca ataaagaaga ccatctggag
     6301 tgcgaagaat gcggcatgtg catcgacatc tgtcctgtcg gggcgctgac ctctggcgct
     6361 taccgctaca agacgcgccc ttgggagatg aaacacgtcg gcaccgtctg cacgcattgc
     6421 ggcgacggct gcaagacgac tctcggcgtg cgtcgcgccg ataccggcat ggagatcgtg
     6481 cgcggcgaca atcgcgacaa gagcggcatc aatggcgatt tcctctgcat caaaggccgt
     6541 tacgctttcg actttgccaa caacagcgag cgcctcagca cgccgctgat taagaaggac
     6601 ggaaagttcg ctcccgcgaa gtgggacgag gctttcaacc tgatcgcctc gaagttcaaa
     6661 gaagcgctgg ctagctccgg cctaagttca gtcggggtga taggctccaa tcgcaccagt
     6721 aacgaggaga actacctgtt gcagaagttt gcgcggctgg tgctgggcac taacaacata
     6781 gaccaccatc gcaccgccga cttccctgct ttcgcctcgg cgctggctgg aaaaccgact
     6841 gccactgcgt cgatgcgcga cgtcgcgact gctccggcga ttctgctgat tggaaacaat
     6901 ccgacagaac agcatccgct gctggcatgg cagattcgta ccaacgtgcg attgcatcgc
     6961 gccaaactct acgtggtgaa tttttcgccc atcaaattgc gccgccaagc gacggcattc
     7021 gcgcagatcg cgcccggctc cgaaggcggc ttcgtacagt tcctgacggg caatgattcc
     7081 gccgccgatt ccgtcacttc cacgcatgtc acgcgcgaag ccctcaatga actgcgcgag
     7141 aagctgaagg tcgagcaggg tctggtcatt atctttggct cggaattacg cggcgccgat
     7201 gtaaagacgc tggtggattt cggcctggct cagggtgcca ggtttgtctg tctcggcgac
     7261 tatgccaatt cgcggggcgc gtccgacatg ggactctatc ccgacctgct gcccggttat
     7321 gctcccgtag acagcgccac caaattccac gagaagtggc tggacgccat ccccaaggct
     7381 gcaggcctca acctgctaca aatggccgac gctgcaaaag acggcagact caaggcgcta
     7441 tatgtagtcg gctcgaatcc ggtcgcacgg tacaacatcg atcccttcgc gttgtcgaac
     7501 gcgtttgtcg tggtacaaga catgttcctg actgagaccg cgatgatcgc tgatgttgtc
     7561 cttccggtcg ccaatgcgta cgagaagtcc gggacctata ccaacacggc cggcgatctt
     7621 caactgctga agaaggctgg cgactttgcc ggcacccgca ccgactttga gtgcatagtg
     7681 cgcatagcgc agaccatggg ctacgacgtg agcaaactgg ttcccttcgg caacaccggc
     7741 acgcgcgcgg acatgggaca gacgcgcgga gcgcaatcgg gcgagtccga tcagaacgcc
     7801 gtatggctca cggcccgtaa ccttgaatcc aagatgagcc cctacgatcc tgtcgcaatg
     7861 ctggacgaaa ttcaaaggct ggttccgggc tatgacgttt cgcgccttgg cgtgctggcg
     7921 ggacaagatg agcacacgca actggtgcag atcacgggtg cgactgggac ggggcatccc
     7981 gaactgattc tgccgtctgg tgacacatta tttacgtcgg gcacacttgg acggtattcc
     8041 gcgacgctca actctgtgat ggagcgcaac cagacttcac cggctgacaa ggaagtcgcc
     8101 gctgactagc agtgggcgcg cggtcttgcg cctgtccttt gtaggtagcc gtaggcaacg
     8161 cggcaggggc cgcgcgaagc gaagcaacga aaacgtattg actcaatgac cataggaacc
     8221 ttcattctcg tctccttgat caagattgtg atcgccatgt tcgtgctgct caccggcgtg
     8281 gcctacacga ccttggcgga acgcaaggtg gtcgcgcaca tacagaaccg ctggggacct
     8341 tcgcgggtgg gcccgttcgg catctggcag ccgctggcgg acggcttgaa atttatcctg
     8401 aaagaagaca ttactccgcc ctttgcaaat aagcccttat tcctgctggc gccaatccta
     8461 tctttgaccg tcgcactgac ggcgatcgcc atggtcccga ttggcccctg ggtcacgatt
     8521 ggcggaatcc acacgccgtt gcagattgcc gacgtcaaca tcggtctgtt gatcattctg
     8581 ggcctcactt cgatcggcgt atacggagtg gcgctggccg gatggtcgtc caatagcaag
     8641 tactcgatga tgggcagcct gcgcgccacc gcccagatgg tgagctacga aattgcgctg
     8701 ggtctgtcgc tcgtaggggt gctgatttac gctggcagtt tcagcctggc cgatatcgtc
     8761 aatgcgcagt cggggacgtt tctaggattt attcccaagt ggaatatttt taccggccag
     8821 ttcgtggcct tcttcattta tgtcatggcg gcttatgccg agaccaaccg cattccgttc
     8881 gatctggcgg aagcggaaac agaactagtg gccgggtatc acaccgagta cagctcgatg
     8941 aagttcgcca tgtttttcat ggccgagtac gccaacatgt tcaccgtcgc gtgtctggct
     9001 accgtgttgt tcctcggggg ctggtcgggg ccgatttttg gtccgccgat tctgcaggct
     9061 ttcttgccga tcgtctggtt tgtggtgaag gtgctggcct tcatctttgt ttatatctgg
     9121 gtgcggggca cgcttccccg cttccgttat gaccagttga tggcgtttgg gtggaagttt
     9181 cttttgccgc tggcgattgc aaatattctt gtcaccagcc tggtggtggc gctgaagtag
     9241 tgcacagttg cagtgagttg atcccggtgg gcagttggcg ccgggttagt gaaaatcatg
     9301 catcttgtct tgttccttat tttcggcgcg gtatgcgtcg gagcggccat caatctgctg
     9361 gtgcagaagc atcccatcaa cagtgcgctg tcgttgatcg tggtgatggg ttcgctggca
     9421 ctgctttacc tgctgctggg cgcggagttc gtcgcggccg tgcaagtcat tgtgtacgcc
     9481 ggcgcagtaa tggtgctgtt cgtcttcgtc atcatgctgc tgaacgcagg ggaggaagag
     9541 cgcactccgc atggcagccg cgtagcaacc ctgctcggtg tgccgggact catcgtcgga
     9601 ctgagcatcg gcatatgggt ggtcactcac tacgcaggat tggatcgcat cgccataggc
     9661 ggaccggcaa tactcaatgg taccgagggc tttggcaatc cgcgagcggt cgcgcaactg
     9721 ctcttccgcg actacctgct tcccttcgaa gtcacgtcgg tattgattct catcgcgatc
     9781 atgggcgccg tggtcctggc gcggagggag gaataaatgg tccctctgtc ctactacctt
     9841 attcttagcg cgattctgtt tgcctgcggc gtcacaggat tcctaatcaa gcgcaacatc
     9901 attaccatct tcatgtcgat tgagctgatg ctcaacgctg tcaacctgac gttcgtcgcg
     9961 ttcgccgcgg agttgagaat gctcagcggg catgtgttcg tgttcttcgt aatggtggtg
    10021 gccgctgccg aagctgctgt gggccttgca atcataattt ccgtattccg cacgcgcgaa
    10081 acgctgaacg tcgatcgcgt gaatctcctc aaactatgag ccttcatctc tggatcattc
    10141 cgttgttgcc gcttaccggc gctgctatta atgggctgct gggcaagcgc tttcccaagg
    10201 caattgtgaa tgccatcgct ctcggggcaa cggcctctgc cttcgcctat gcgctctacg
    10261 tcgcagttca gttcatgggc ctccccgccg accagatacc acatatcgag cgctatgcca
    10321 cgtggatgac cgctggcttg ttcaccgccg agtacggtat ttacctcgac caactctcgc
    10381 tggtcatgct gctgatcgtc accggtgtcg gcttcctgat ccacatttat tccgtgggct
    10441 acatggagca tgaaggcggc tactaccgct acttcgccta cctcaacatc ttcatgttct
    10501 tcatgctcac cctcatcctg gcgaacaatt acctgttgat gtttgtcgga tgggaaggcg
    10561 tgggcctggc ctcgtacctg ttaatcggat tctggttcct caaggattcg gcggccaacg
    10621 ccggcaagaa ggctttcatc actaaccggg ttggcgattt cggattcctg atcgcactct
    10681 ttctgctgat taaacatttt ggaagcctgc aatacgaaac cgttttcacg gctctgtcgg
    10741 ggtatccagt cgaagcctcc gccggcctgc tgaccgcaat cggactgctg atgatggtgg
    10801 gagcgagcgg aaagtcggca cagattccgc tctacgtctg gctgcccgac gcgatggaag
    10861 gccccactcc cgtttccgcc ctgatccacg cagcgacgat ggtgacggct ggcgtttaca
    10921 tggtggcgcg ctccaatgca gtcttcaatc gcgcgcccat tgcgttgacg gtggttgccg
    10981 tcatcggcac actgaccgcg atcttcgccg cgtcgatcgg catcgtgcag accgacatca
    11041 agaaggtgct ggcctactcc acgatttcgc aacttggtta tatgttcatg ggatgcggcg
    11101 ttgcggccta cggggccggc atctttcact tgatgactca cgccttcttc aaggcactcc
    11161 tctttctgtc ggccggagcc atcattcacg gactcggcgg ccagcaagac atgcgccaca
    11221 tgggcggcct gcgcaagtac atgcccaaga cgttctggtg catgacgatc gcgacattca
    11281 ccatcgcagg cttccctccc ctcgcggcgt tcttctccaa ggacgaaatt ctctgggcga
    11341 cctgggctag cggaaaccac gtgctgtggc tgattggtct cattgccgca gccatgacgt
    11401 cgttctacat gttccgactg tggttcatga cattcttcgg cgagtaccga ggtgctacgg
    11461 cgggagaatc ccagcacggc cacgacagcc acgatgcgca cgcacacggc acaccgcacg
    11521 agagccccat ggtcatgatt cttccgctca tgattttggc tgtgctgtcg ttcgtcggcg
    11581 gatgggtcgg tgttccagca gcgctcggtg gctccgatcg tttcgatcac ttcctcgacc
    11641 ccgtcttcgc agcccactcg ccagtggaga ctaaattggt ggccccgacc aacgaagagt
    11701 tggctgcgca tcacgaggaa accggtactg aactgctctt catgggaatc tcagtgagtg
    11761 cggctgccct tggcttcctg ggcgcgtggt tcctgtacta caagcgtact gatcttcccg
    11821 ccaaaatggc agcggccgcc ggtggcctct acaccttcgt actcgacaag tacaaggtgg
    11881 acgaattcta cggcgcggcc gtcatccggc cgattgtgct ggcctccacc aacattctct
    11941 ggaaaggcat tgacgccacg ctgatcgatg gagccgttga tgaaagtgcc gtcggcgcac
    12001 gtgacatctc cgacgggtta cgtcacatgc aatccgggaa catacgctct tacgccggat
    12061 gggtcgcctt cggcgcggct gtagtcatcg ccttcatggt gtggaagggt gtgcgatgaa
    12121 ctacatcgac aacaacatca tcaccatcat caccttcctg ccgtttgccg gtgccctgtt
    12181 gctttgcatc tttccccggc gcgatcgcga tatccgctgg ttcgccctgg gcatctcgct
    12241 ggtaacactg ttggcctcgc tgcatcttcc ctggcactac gtgcgcgggc tcgatggctt
    12301 tcagttcgaa caaaacgttc cctggatttc gcatcccaac atccactacc accttgggat
    12361 cgatggcatc tccatgtggc tggtggtgct gaccaccttc ctcacgccgc tatgcgtgct
    12421 gatttcatgg aagtcggtga acagccaggt caaagagttc ttcatcctga tgctgattct
    12481 cgagacggcg atgattggcg ttttcgtcgc gctcgacctg ttcctcttct acttcttctg
    12541 ggaagccacg ctaatcccga tggcgctgat catcgggatc tggggccatg gccggcgcat
    12601 ctacgccgcg gtcaagttct tcctgtacac catgatcgcc tcggtcttca tgttggccgc
    12661 gatcatctgg ctctacaccc acactggcag cttcgatttc gtcaccatcc gcgatgccat
    12721 tcaaagtggg caggtggaag gcttctccac tgccgcgaag tggctgttcc tgggcttctt
    12781 cgttgcgttt gccgtcaagg tacctctctt tccgcttcac acctggttgc ctgacgcgca
    12841 cgttgaggct cccacggccg gctccgtcct gctggctggt gtgctgctga agatgggaac
    12901 gtacggcctg ctgcgtttcg ccgtcggcct cttccctgat caggcgcgca gcaacgctcc
    12961 gtggatcatc gtactggcca tcatcagcat catctccggc gcactggtag ccatggtgca
    13021 gcccaatgtg aagaagttga tcgcctacac ctcggtcagt cacctgggat tcgtcgtgct
    13081 cggcattttc agtttcaccc agattggcat ggacggtgcg gtgtaccaga tgctcggtca
    13141 cggcatctcc actggcgcgc tgttcatgtt gctgggcatg atctacgagc gtcgccacac
    13201 ctatgagatt tccgagtatg gcggcatcgc tactccgatg ccggtgtatg cgaccttctt
    13261 cacgttcatc atgctgtcgt cggttggatt gccgctgctg aacggattcg tgggcgagtt
    13321 ccttatcctg agcggcgcat tccaggcccg ccagatttac ggcatcctgg cggcaaccgg
    13381 cgtgatttgg agcgcatgct atttgctgtg gatgtaccag cgcgttttct acggcaacat
    13441 cacccatcca gtgaacaaga atctgcagga tatggatgcg cgcgagcggg tcgcgatgtg
    13501 gccgctggca gtcgccgcgc tcgtcatggg agtggctccc aatctatggc tgcactcgat
    13561 tgacccgtcg gtatcgttcg cgctcaagca attttctagt ggagtacagg ccgctgcgcc
    13621 ctcggcctcc cacgttaccc aagctcttat gcaggtgatc ggacgatgat tccgaccgtt
    13681 gactatatcc ggatccttcc tgaagtggtg ctaaccatct tcggcatcct ggtcatgctg
    13741 gtggatccgg tcattcccac acaggactcc aagaagccgc taggcgttat agcgctaatc
    13801 ggcgtgctgg ccggtctggc tgcgacgctc tatcagtcgc gcttctatgg cgacgcgttc
    13861 taccacatgg tgcgcgtcga tgccttcagc atcttttttc atcttgtcat tcttcttatc
    13921 gcgctggtcg tcattcttag ctcgtacgat tatctcgaag ttcagcagat caaggccgga
    13981 gaatattacg ggctcgtgct gttcgccacc gttggcatga tgctgatgtc gtcggcgatt
    14041 gagttggttc tcatcttcat cgccctggaa atctcttcga tatcgactta catccttgcg
    14101 ggcttccggc ggcgcgccgc tgaaagcgcc gaagcttccc tgaagtattt cctgttggga
    14161 tcgttcgcta cggcgttctt cctctatggc gtcgccatgg tgtttggttc gactggatcg
    14221 accaacatct atctgattgc cgaaatcctg aagactcacg tttcggttct gacctatctt
    14281 gcggttgccc tgatgtttgt cggactgggc ttcaaagtcg cctccgctcc ctttcacgtt
    14341 tggactcctg atgtatacga aggctcgccc gcgccagttg tgggattcat gtccaccggc
    14401 ccgaaagcgg ctgcgttcgc ggttctgttg agggtgcttt tcgccaccgg cgctccgggt
    14461 tggttctggc tggtctggat ctcggctgtg ctatccatgt cgatcggcaa cattggcgcg
    14521 ctggtgcaaa acaacgtcaa gcgactgctg gcgtactcct cgattgcgca cgccggatat
    14581 gttctcgtcg cctttgccgc cgcgcagacc atgggtatct ccgcagcgat gttttacgct
    14641 gcctcctacg ctgcaatgaa cgtcggagcg ttcgccattg tcgcccatct ttcgggcaag
    14701 ggcgagaagt acgtgaccct cgaggactat gccggactgg gccgccgttc cccgctgatc
    14761 gcgtcgatac tcaccatctt tttgttgtcg ctgattggca tccccattac cggtggattc
    14821 ttcgccaagt tctacgtttt ctccgcggcc atgcattcca acctcgtagt tctcaccatc
    14881 atcggcgtca tcaacagcgc cattgccgcc tattactatc tgcgcatcat tgtgtacatg
    14941 tacatgcgtg acgagcgcgc cgtcgaaccg ccggtcgccc cgattccctt cagcctggga
    15001 gcgggactgc tcatcagcct gattgcagtc atctacctcg gcgtgcttcc caacgacgtg
    15061 ctggattacg ccatccgcgg cgcgcaggac ctgctgaagt agttcttggc gagtcacaac
    15121 ttattccgca aaagcaaatg ccctgtccga agacagggcg tcaacgattt gccgaagccg
    15181 aaaactagac gaccttggcg aagatgatga ccagcgtgaa cagcaccagg gactcgatga
    15241 acgccaggcc gagaataagc gcgagctgaa ttccaggacg ggcgcccggg ttacgcgcca
    15301 tgccttcgca ggcggcagcg cctactttgc cctgacccag agcggccagg ccggcggcga
    15361 ttgccatgcc gaagcccgag gcaatcgcga cccagttggt agttggtgca gcggcggctc
    15421 cgccctgcgc gtaggctggc atggtcatca tcatgatggc cagcagcgag aacattccaa
    15481 tcgacagttt ccgcattgtg tttctcctta tgaaatgctg ccagtgggtg gttgacgtca
    15541 tcctcgagca gacgccctca cgctcgcagc aaacttgttt agtgttcgtg cgccaccgct
    15601 tcgccgatat acaccgtcgc cagcagcaca aaaatatagg tctgaataaa cgagacgcca
    15661 acgtgcagca gcatgaaaag taccggaatc gcgagcggga acagtgagaa gaacaccaga
    15721 gtcaccatgt cgcccgcgaa catgttggca aaaagacgga cggtgagcga caaaattcgc
    15781 gccaggtgac ttgcgacttc gatgacgaac atcaggggcg ccagccacca cacgggtccg
    15841 agaaaatgct tgatgtagcc gggcccgttc gcgcggatac cgtgcatctg gtagtaaatc
    15901 cacgtcacaa ccgcacatcc gagcggcacg gacggcaccg ccgtaggcgc ttcaaaaccc
    15961 ggaatcaatc cgatcaaatt gcacgttaga atgaaaatcc ccagcgtgat caggtagttg
    16021 gtgaatcgct catagccatg tccgatgact tcaagaccct gctggctgat cattgcgtcg
    16081 atgctctcga atacgtgctg cagattgccg ggattctcca ccgacaaccg cgaacgcacc
    16141 aaagcaaaga tgaggaccag cagcacgaac accaccaact gcatggctac gtagttggtg
    16201 atcggcgctt ggggatggct ggaatggaaa tgaaaaacgc ccagaatcgc gttggtcact
    16261 ccggcgaggt acttatttag aaatgcagta acccaaagct gctcaggcat aaatctcgcg
    16321 accgtaaatt gtattaatcc ctgctgttgc gtatctcgac gcgcgcccgt atgaactcaa
    16381 gcgcatgtcg ttaagtaagt tcgggaaatc agaatccgcg ccgcagcccg acatagactt
    16441 ctaccacggc ctcgcaggcg attgcggcga tggtgaggca aacgcccccc aggaagccgt
    16501 acagtcctgc cagagaaact ctaaatataa catatgctcc cagcgcgatg aagaggtatc
    16561 ggagcacggc gcgaaccacg aggatcccgc ccctttcgcg gctctcgcca ctggtaatgc
    16621 gctcgccaag ggcctcaatc atccgctcca accacacatg gttcacgtag gagatgaccg
    16681 ccccaaccag aaatcccgcc gccaccaccc ggccgaaact gagcaggcaa accagcactc
    16741 cggcgccacc gagtatcacg atgaagcggc ggatccgacc taacgctgca ccgtagaatt
    16801 tctcggctgg actatcccga gcgctgggtt ccgttgctga cataccgcag ataactgtac
    16861 cgcacgacgt cccggttgac agtcattcgt ccgctaaagc ggctttatct tcgcggctga
    16921 cacctgtttc acaacttcct cgatgttcaa agtcgaattc gctgccgtgg cgaacgcgac
    16981 cgagaggact ccctggcccg acgcaattcc accgggagca aactgtaccc acccgaaacg
    17041 catccgctgg tccttgtagg ccgccggcat gtgaaagtgc ttgatcacgg tttcagaaat
    17101 gggcttctcg ttctccactt cgaacaacag agcgtcgcca cccggaactg aacgttgctc
    17161 tgctttggtg acgcgtaccg gaagaaaatc ctccctcacc tcggaagaac caccggaatc
    17221 atgcatttga tacgcatcga acaattgcga gacgtaaaac cacagggggt tcccttcgcc
    17281 atctttcatg tccgaaatcc tgggaggctt cgttcccttt gccagatctt tcgaagcctt
    17341 ttcggcgtac agaaacggaa gggggaatgc ccccgccgac atcactccag ccgcgtcctt
    17401 gctgctagcg ttgatgccgg tgaagaactt tgcgatctcc ccggccttat gcaggcccgc
    17461 ctgttggctg atttcgatta cagcgccggt accaggatcg taaaagatgt ctgaccccgt
    17521 ctcgacggtt gcatacttct tccctgactg ccactgtccg gaaatcgaca aatccgcata
    17581 ggtgctgagt atttcctgag aaaaacaact gaccgtgaga gcggcgatca gcaacagaag
    17641 gaagccgcga aggataagac gaatccacgc cgcaattcta tacacaagaa cctccactat
    17701 catgcataag accaaacact cgaagtcgca aggtaatgtg caccctgcga gggtgacatt
    17761 cccgaatcgg gaagtaacag gcggctgcac agaacggttg actgatggcc cgtcagtcct
    17821 tagcgcgtgt aaccatgaca atcatctgcg cgaaccctac cacggcgccg acgatgattc
    17881 cgccgagaaa aatccagcgc gtgtgcaacc aatagtcgag cagtttgcca atcaaaagtc
    17941 cgacgaccac cgcggcaggg atgataaacg cgatctcgga gtactttgcc caaatcaccg
    18001 ccgggttgtc tgaaccctta ttgctgcgct cgtccgacat ggagccagcc taacaccgcc
    18061 cgccagaaaa tctacaatcc tatttctcgt ccctctccga tgaggtaccg cagttccctt
    18121 cacggcgaga tccaccagcc gccttgacac tgtttgacat caacagaagc gtcgaccgga
    18181 cgacacattc acagctgccg aacgccatag ctattacaga cgacgaacat tcgtgcgcgt
    18241 cttcggcaca cacacgcaat ttcgatcgac acaacagctc gttggtttga cctgatcttt
    18301 ctatctcggt atgatcgaag tgcggggtgg agcagtctgg tagctcgttg ggctcataac
    18361 ccaaaggtcg gtggttcaaa tccacccccc gcaaccaaac aagttcctga ttacaaacgt
    18421 cttccgtcgc agccggaaga cgtttttctt taacttgctc acaatttgct cacagaattc
    18481 cagccgcgaa tttcgcatct acatgatcct cccttccgtt tggttcgccg gcaaaggaca
    18541 aaacaagtcg tcgagttgct ccaccaccct gcggtcgtcg tctccgacca ggtgcgtgta
    18601 gcccatagtt gtcctcgagc tgacgtgtcc gagacgctcc tgccttactt tcatcggagt
    18661 gtgcaggcg
//
LOCUS       DASRKK010000446        16517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_914810, whole genome shotgun sequence.
ACCESSION   DASRKK010000446 DASRKK010000000
VERSION     DASRKK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914810"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFC15_10445"
     CDS             <1..231
                     /locus_tag="VFC15_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10618.1"
                     /translation="VLHRKNALFYRTLNGAQVGDLFMSLIHTCQLCGVNSFDYLVELQ
                     RHAQELAARPAEWMPWNYRETLARTVNLPDPA"
     gene            297..953
                     /locus_tag="VFC15_10450"
     CDS             297..953
                     /locus_tag="VFC15_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM10619.1"
                     /translation="MAGKRASSKPDSPRVFGQGAGRRETPCRVGLYARVSTHDQQTLP
                     LQSRAMRNYVARRGWSIVVQVQEVASGTSQRELREKVLDAARRREIDVVLVWRLDRWG
                     RSVADLVATLQELSHLGVGFVSLTEALDLTTPIGRAMAGLLAVFAEFEREVLRERVRA
                     GLANARQNGKRLGRPPTAARKGGEVRELYRHGVSQAAIARRLQIGRTSVRRILKSKRV
                     "
     gene            955..1563
                     /locus_tag="VFC15_10455"
     CDS             955..1563
                     /locus_tag="VFC15_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HZM10620.1"
                     /translation="MPTKKTASPQEIYQIKVTLLGTRPPIWRRLLVPANLTLEQLHDV
                     LQLAMGWEDCHMHEFRIGQKRFGTPDPNERLMGLPSTASERTARLFNVLGKVGAKAVY
                     TYDFGDGWEHGIAVEKVLLPEAGHAYPICVDGKRHGPPEDCGGVPGFYNLLEAISNPE
                     HDEHEEMRDWLGDDFDPEVFSVDEVNRQLAPLLRRRSKVEPT"
     gene            complement(1581..1835)
                     /locus_tag="VFC15_10460"
     CDS             complement(1581..1835)
                     /locus_tag="VFC15_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10621.1"
                     /translation="MASSEVNGEPSAAGIGGFLAAAAVLVATWAMLGTAVAQREATPK
                     PQDKLALGEAEVKQLLLLMDTDKNGKISVSFRQACIAEAR"
     gene            complement(2104..4542)
                     /locus_tag="VFC15_10465"
     CDS             complement(2104..4542)
                     /locus_tag="VFC15_10465"
                     /inference="COORDINATES: protein motif:HMM:NF003617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZM10622.1"
                     /translation="MSSIPVSSATTNASPFELTPLTSDELRRMDAYWRACNYLCAGML
                     YLRENPLLREPLKPEHIKNRLLGHWGSDPGQTFTWVHLNRLIKKYDLDLIYVSGPGHG
                     APATLANSYLEGVYSEVYPEMGEDAVGMQKFVKQFSFPGGIGSHCTPETPGSIHEGGE
                     LGYSLSHGFGAAFDNPDLIVTVVVGDGEAETGPLATSWHSNKFLNPIYDGAVLPVLHL
                     NGYKIANPTILARISSQELESLFIGYGWTPYVVEGSDPALMHQEMASVMEHCVLEIRA
                     IQEKARSAAAGVATERPRWPMIILRTPKGWTGPKELDGHKVEGSWRAHQIPILDPVTN
                     PAHLKLVESWLRSYRPEELFDANGTLLAELKDTVPRGARRVSANPHANGGTLRKPLDL
                     PDFREYAVKVAQPGQIEVSSTDTVAHFLRDVMRKNMKSFRVFGPDETASNKLQVIYEA
                     TGKTWMAEMMPEDADGGDLSTDGRVMEMLSEHTLEGWFEGYVLTGRHGFFSSYEAFVH
                     IIDSMFNQHAKWLEKSKLELRWRAPISSINLLITSLVWRQDHNGFTHQDPGFLDLVTN
                     KSPEVTRIYLPPDANCLLSVTDHCLRSTNYVNVIVADKALHLQYLDMDEAVKHCTKGI
                     GIWNFASNDAGQEPDVVMACAGDIPTSESLAAVAILRERCPDVKIRFVNVVDLFRLMP
                     EREHPHGLSDRAFDSLFTTDKPVIFNFHAYASLIHKLTYRRTNHENIHVRGYKEKGNI
                     NTPLELAILNQVDRFNLAIDVIDRVPKLQATASHTKEWLNDQIVDCVNYAHENGIDRP
                     AIREWKWPGGVSQP"
     gene            4950..5150
                     /locus_tag="VFC15_10470"
     CDS             4950..5150
                     /locus_tag="VFC15_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10623.1"
                     /translation="MPQSPHDRAAELHNLAAHAHAAAATAHGKGDHLTAHELSKKAHE
                     HSMNAFKHANEAFPAAAGGKTP"
     gene            5199..6443
                     /locus_tag="VFC15_10475"
     CDS             5199..6443
                     /locus_tag="VFC15_10475"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZM10624.1"
                     /translation="MNVFVLNSGSSSLKFQIIATDLDRIRQHQDERICRGEVERIGGE
                     AIVTFQKGGDAKQKFTAPLRDIAAALDYLVRYIASDKSGVTEIKSSGDIHAVGHRVVH
                     GGEMFSESALIDSKVLQDIEDCIDLAPLHNPNNIKGIVAARELFGKDVPQVAVFDTSF
                     HHSIPEQAFLYALPYHLYRRHRIRRYGFHGTSHRYVAFRYRALRELPREQTNIITLHL
                     GNGCSATAIRKGNSVDTSMGMTPLEGLVMGTRSGDLDPAIVNLIATKEGMSPSEVDAL
                     LNTQSGLLGISGLSNDMRMLQAELQENHDRRVQLAIEVFCYRARKYIGAFLAAMGGAD
                     AVVFTGGIGENSPEIRAHICEGMEWAGLTIDPVKNQQTVGREGLISTEDSKLVAYAIP
                     TDEELLIARDTVRVILGEPHPS"
     gene            6586..9129
                     /gene="mgtA"
                     /locus_tag="VFC15_10480"
     CDS             6586..9129
                     /gene="mgtA"
                     /locus_tag="VFC15_10480"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZM10625.1"
                     /translation="MGKPATSDESFWDRPLHELFELLPATPAGLTTEEARRRLRLYGP
                     NSFVHESRFAALLSFFGFFRNPLVVILVIASGVSLALGEHVGGLIILAIVLFSVILNF
                     LMEFQARHAVEDIQKQIALTAAVVRDGREQELPTAELVPGDIVRLNAGDLVPADARLL
                     EVKDLHVRESVLTGESLPVEKTVADLPKAKHSVADASNSVFLGTAVQTGIGTAVIVCT
                     GKDTACGEIANRLAMRPPETEFGRGIRHFGMMLTWVTMLLVLFVLLVNIIFHRPVLES
                     FLFSVALAVGMTPEMMPMIITVTLAQGAKRMTKKKVLVKQLSAIEDFGSIDILCTDKT
                     GTLTEGEIVLDRHVDFQGKDNDNVLQLIYLNSHFEAGIKSPLDDAILKHEAPSIEGYD
                     KVDEIPFDFNRKRLSVVVRHTDEDLLITKGEAESVFAICETVTVDGVAQPFDEKRRAE
                     AEDTLKKLSADGFRVLGVATLKAQKQDAYAVAAEHAMTLVGFAAFLDPPKEGVLAVLQ
                     ALKQNGISVVVMTGDNQYVTQKIAHDVGLDTDHILVGNQLDTMDDAAVAHQAENGAIF
                     ARVSPEQKNRVILALKARGHVVGYMGDGINDAPSLHTADVGISVMNGVNVAKDAAKII
                     LLEKDLAVINEGVLEGRRSFANIMKYIIMGTSSNFGNMFSMAAASLFLPFLPMLPSQI
                     LLNNFLYDVSQVSIPTDNVDPAMVQKPKRWQIGFIRQFMAIIGPISSIYDFLTFGVLL
                     WAFHASTNESLFHTGWFVESLATQTLVVFVIRTAANPLKSRPSRPLVISVLAIVTVAT
                     VLPYTPLGTLLRFTPLPVSLLVAIAFLAVTYLFVVQAVKSWFYRRHALL"
     gene            complement(9182..9877)
                     /locus_tag="VFC15_10485"
     CDS             complement(9182..9877)
                     /locus_tag="VFC15_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZM10626.1"
                     /translation="MAAGNVERHRTQRIGWLRAAVLGANDGILSTSSLVLGVAAAHAN
                     HRNVLVAGVAGLVAGAMSMAAGEYVSVHSQADTEQADLKLERAELKADDNGEHKELQA
                     IYVKRGLKPALAKQVAEQLMDHDALGAHARDELGINETLRARPIQAALASAGSFAIGG
                     VLPLLVTAVAPEAALIPLVAGTSLLFLALLGGLAAHAGGARVMTGAIRVTFWGALAMA
                     ITSGIGWVFGTVA"
     gene            complement(9977..10801)
                     /locus_tag="VFC15_10490"
     CDS             complement(9977..10801)
                     /locus_tag="VFC15_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM10627.1"
                     /translation="MTNAIAQRNFNPRTFLTSIGSGRRMMPFQKNHVIFAQGEPSDGL
                     FFIQEGKVQLSVVSEAGKEATLGILGDDDFFGEGGLAGQLVRMSSATAMTDCVLLHVE
                     KKAMMLAMSREPKLSATFVKYLLKRNVRYQDDLIDQLFNSSEKRLARILLLMAHFGKE
                     GVVTEIAVPKLSQETLAEMVGTTRSRVSFFMNKFRKLGFINYDTGHHLRVHSSLLSVV
                     LNDDGGTAIPGAAKLTANGKKALEPPAEPGEPPEKERAHRPPLSKPSRALELNAPK"
     gene            complement(11475..11654)
                     /locus_tag="VFC15_10495"
     CDS             complement(11475..11654)
                     /locus_tag="VFC15_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZM10628.1"
                     /translation="MKPSTEDKTTGKLHEAKGTIKEKAGELINNPDLEADGRAEKNAG
                     KAQNWVGKVENLVGE"
     gene            complement(11726..12301)
                     /locus_tag="VFC15_10500"
     CDS             complement(11726..12301)
                     /locus_tag="VFC15_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10629.1"
                     /translation="MNLNLMDPKLIAFAVVLAVVIVVAVALYMRKRSNTTTGLKNRFG
                     PEYDRAVKQHGSERKAEAKLADRETRVERLNIRDLDLTERERYLALWQTVQSRFVDFP
                     KGAVTEADELVCSLMQTRGYPVTDFDQRAADISVDHPRVVENYRSAHNIALRLGAGDA
                     STEELRTAMIHYRSLFDELVQVQTPGDKKVA"
     gene            complement(12609..12782)
                     /locus_tag="VFC15_10505"
     CDS             complement(12609..12782)
                     /locus_tag="VFC15_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10630.1"
                     /translation="MSFGLYSIGFAIVIAGLIYGAHLMHIPTHWIAVGAVVLLGVGIL
                     SGVKATRQKDPAE"
     gene            13160..14716
                     /locus_tag="VFC15_10510"
     CDS             13160..14716
                     /locus_tag="VFC15_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZM10631.1"
                     /translation="MKSTAMFLPCLMVLGASVLAMPPPTQAQSSDPGTKQTTQVVTTT
                     TRTTTFHITVVSRTVQAVNYRHRGGATKLDFAGTNLMPAATGEAKVESKKGYIEIEVE
                     FARLARPTTFGNEYLTYVMWAISPEGRAVNLGEVLVGDNERSKLDVTTDLQAFALIVT
                     AEPYYAVRQPSNVVVLENIVREDTKGTSEVVKAKYELLERGGYIPTGYTLDPVVLNAN
                     LPLEFFEARNALRIAQSVGADRYAVASYQHAVSLMNKADAYATSKHIDKKELISVSRE
                     AVQTAEDAREIAVKKLIADNQSNELQASARAANDATRQRDLATAQAAKAKADMERGQA
                     DSANQVAAAHAETERARLATAQANVQVQAAENDKVAMRERLSLQLNTILQTRDTARGL
                     IVNMSDVLFDTGKYTLLPGAREKLSKVAGILIAYPGLNIAVGGYTDNVGSDQFNQGLS
                     EHRASSVRDYLVQQGVLANSITSNGFGNTMPVASNDNSAGRQENRRVELVVSGESIGT
                     DVNVTTGSLQ"
     gene            complement(14863..>16517)
                     /locus_tag="VFC15_10515"
     CDS             complement(14863..>16517)
                     /locus_tag="VFC15_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZM10632.1"
                     /translation="AADGGRDYDSSYIPEELMWNVYLPPFKAAVDAGVGSLMSAYMDL
                     NDVPASGNKWLLTDVLRRAWSFEGFVVSDAVAVHSLVTHGYARDSQDAAYKAFSAGLD
                     MDMASGTYWKFLLEEVQQGRISMQQIDNAVRSILEVKIRMGLFEHPYSDESRSAQVLN
                     DPQHRQLARIAAQRSVVLLRNNKNLLPLDRSNLKSIAAIGPLADAEDDLLSMWGHMTK
                     PGPTVSVLQGIRDKVGTAVRVDFAHGPNLRRDFPSPFETIPISPMKEQPQQASEEARK
                     AIDEAVALAKQSDITVVVLGELDLMSAESASRSSLRLSNGQQEMLEAASALGKPVVLV
                     LVNGRPLDISWAAEHVPAILEAWYPGSQGGNAIADVLFGDASPAGHLPVSWPRSTGQV
                     PIYYNHNLTQLHETSPDFKSRYWDVLSTPLYPFGYGLSYTTFAFDNLKVSRVEAKVGA
                     NVEVSADVRNTGSRAGDTVVQIYIHQRAGSASRPVRQLKGFERVTLEPGAKRTVHFQL
                     GPEELQFWSPTKKQWVVEPEQFDVWIGEDSTAALHGAFRLTQ"
BASE COUNT         3497 a         4857 c         4638 g         3525 t
ORIGIN      
        1 gttctccatc gcaagaatgc gctgttttat cgcaccctga acggagcgca ggtcggcgat
       61 ctgttcatga gcctgatcca cacctgccag ttatgcggcg tgaactcctt cgactacctg
      121 gtcgagctgc agcgccacgc ccaggaactg gccgcccgac ccgcggaatg gatgccatgg
      181 aattatcgcg agactctggc gcggactgtg aatcttcccg atcccgcata gcattatgat
      241 gaatcccaca tggccggaaa ggattgcttt cggccatacg caggagcccc tcggaaatgg
      301 ccggaaaacg ggcctctagc aaacctgatt cgcccagggt ttttggccag ggggcgggcc
      361 ggagggaaac tccctgccga gttggcctgt acgcccgcgt ctccacccac gaccagcaaa
      421 ccctaccctt gcagagccgt gccatgcgga actacgttgc ccggcgagga tggagcattg
      481 tcgtgcaggt gcaggaggtc gcctccggca catcccagcg tgagctgcgc gagaaagtgt
      541 tggacgcggc gcgccgccgc gaaatcgacg tggtactggt atggcgcctg gatcggtggg
      601 gacgatcggt ggccgacctg gtggcgacgc tgcaagaact cagtcatctc ggagtcggat
      661 tcgtatcgct gaccgaagcg ctggatctaa cgacccccat cgggcgagcc atggccggtt
      721 tgctggccgt attcgccgag ttcgagcgcg aggtcttgcg cgagcgggtt cgcgccggct
      781 tggccaatgc ccgacaaaac ggaaagcgcc tgggtcgtcc gccaacggcg gcgcggaagg
      841 gcggcgaggt acgggagctt taccgccacg gggtcagcca agccgcgatc gcccgacgct
      901 tgcagatcgg acgcacctcg gtgcgccgta tcttaaaatc gaagagggtt tagtatgcca
      961 accaaaaaga ccgcgagtcc acaggagatt tatcagatca aggtcactct gctcggcacg
     1021 cgtccgccga tctggcgccg gctgctggtg ccggcgaatc tgactctgga acagttgcat
     1081 gacgtgctgc agctggccat ggggtgggaa gactgccaca tgcatgagtt tcgcatcggt
     1141 cagaagcggt tcggaacacc cgatccgaat gagaggctca tgggactacc gagtaccgcc
     1201 agcgaacgca ccgcacggct tttcaacgta ctgggcaaag tcggcgcaaa ggccgtgtac
     1261 acctacgact tcggcgacgg ctgggagcat ggcattgccg tcgagaaggt actgctaccc
     1321 gaagcaggcc atgcatatcc catctgtgta gatggcaaac gtcatggtcc gcccgaagac
     1381 tgtggtggtg tgccaggctt ctacaacctg ctggaagcga tcagcaaccc ggagcacgac
     1441 gaacacgagg agatgcgcga ttggctgggc gacgattttg acccggaagt tttttcggtc
     1501 gacgaggtca accgccagct tgcgccctta cttcgccgac ggagcaaggt tgaacccacc
     1561 tgaagcatcg gcccaggtgg ctaccgtgcc tcggctatgc acgcttgccg gaaggacacg
     1621 gagatcttgc cgttcttgtc agtatccatc aggagcagca attgcttgac ctcagcctct
     1681 ccgagtgcca gtttatcctg cggtttcgga gttgcttccc tttgtgcgac tgcagttcct
     1741 agcatggccc aggtcgccac cagcacggcc gcggccgcca gaaagccgcc gattcccgcc
     1801 gccgaaggct ccccgttgac ctccgaggag gccaaaataa ccgcctaagt ccattcggca
     1861 gacatggata gctataacta gtaatagaat gaatgagata tgagcaattt cagcctaagt
     1921 cactgaaaac aaaaggagcg tgtaaaagga aattagatga atcgagcaca gcacttcatc
     1981 gcacctcacg gaagtacatg aagcactgaa acagcgattt tgcgcctggt ccttggcgac
     2041 cttagggcct tgaggcggtc tgcaatcgtt gaccggtatc tcagagataa ccgaagcgtc
     2101 agctcagggt tgagaaaccc cgcccggcca cttccactct cgtatcgccg ggcgatcgat
     2161 gccattctcg tgcgcgtaat tcacgcagtc gacaatctga tcgttcagcc attccttggt
     2221 gtgcgaggcc gttgcctgca acttcggtac ccggtcaatc acgtcgatgg cgagattgaa
     2281 ccggtccact tgattcaaga tcgccaactc cagaggcgta ttgatgttgc ctttctcctt
     2341 gtacccgcgc acgtggatgt tctcgtgatt cgttcttcga taggttagct tatggataag
     2401 cgaagcgtaa gcatggaagt tgaagatcac gggcttgtcc gtagtgaaca ggctgtcgaa
     2461 ggcgcggtcc gaaagtccat gaggatgttc cctttccggc atcaatcgga acagatcgac
     2521 gacgttgacg aatcgaatct tcacgtccgg gcaccgctct cgcaagatcg caaccgcggc
     2581 cagcgattcc gaggtcggaa tgtcgcccgc acacgccatt accacatccg gctcctggcc
     2641 ggcatcgttg ctggcgaagt tccagatccc gatacccttg gtgcaatgtt tcacggcctc
     2701 gtccatgtcc agatactgca gatgcagcgc tttgtctgcc acgatgacgt tcacgtagtt
     2761 ggtgctgcgc aggcagtggt cggtcacgct caacaggcaa ttcgcatccg gcggcagata
     2821 gattctcgtg acctccggac tcttgttggt caccaggtcg aggaagcccg ggtcctggtg
     2881 cgtgaatccg ttgtggtcct gccgccacac cagagatgtg atcagcaggt tgatggacga
     2941 gatgggcgct ctccacctca gctccagctt gctcttctcc agccacttcg catgctggtt
     3001 gaacatcgaa tcgatgatat ggacgaacgc ttcgtagctc gaaaagaatc cgtgccgccc
     3061 tgtgagcaca tacccttcga accagccttc cagagtgtgc tcgctcagca tctccatcac
     3121 gcgcccatcc gtcgacaggt ctccgccatc ggcatcctcg ggcatcatct cagccatcca
     3181 cgttttgcca gtcgcctcat agatcacctg aagcttgttc gacgcggtct cgtccggccc
     3241 gaacacccgg aagctcttca tgttctttcg catcacatcg cgcagaaaat gtgcaaccgt
     3301 atcggttgag gagacctcaa tctgacccgg ctgcgcgacc ttcactgcgt attcacggaa
     3361 atcgggcaga tcgagcggct ttctcagcgt tcctccgttc gcgtgcggat tggcgcttac
     3421 acgtctcgct cctcgtggca ccgtatcctt taattcggcg agtagagtgc cgttggcgtc
     3481 gaacaattct tccggccggt aactccgcag ccacgattcc accagtttca aatgcgctgg
     3541 attggtgacc ggatcgagaa tcggtatctg gtgcgcccgc cacgatcctt cgaccttgtg
     3601 accgtccagc tccttcggac ctgtccatcc cttcggcgtg cgtaagatga tcatcggcca
     3661 gcgtggccgc tctgtggcca ctccggccgc cgcgctcctc gccttctcct ggatcgctcg
     3721 aatctcgagc acgcagtgct ccatgacact tgccatctcc tggtgcatca gcgccggatc
     3781 actgccttcc accacgtagg gcgtccagcc atacccgatg aagagactct ccagttcctg
     3841 cgatgaaatt ctagccagaa tcgttggatt cgcgatcttg taaccattca aatgcaacac
     3901 tggcaatact gcgccgtcgt agatgggatt caggaacttg ttcgagtgcc acgacgtcgc
     3961 cagcgggcca gtctccgctt cgccatctcc cacgactacc gtcacgatga gatcggggtt
     4021 gtcgaacgcc gcgccgaatc catgtgaaag gctatacccc agctcccctc cctcgtggat
     4081 cgaacccggc gtctcgggtg tgcaatggct tccgattccg cccgggaacg agaattgctt
     4141 gacgaacttc tgcatgccga cagcatcctc gcccatctcc ggataaacct ccgaatacac
     4201 tccctcgagg tagctgttgg cgagcgtcgc tggtgctcca tggcccggcc ctgaaacata
     4261 gatgaggtcg agatcgtact tcttgataag ccggttcaga tgcacccacg tgaaggtctg
     4321 tccagggtcg gaaccccagt gtccgagcag cctatttttg atgtgctcgg gcttcagggg
     4381 ttcgcgcagc aacgggtttt cccgcaggta aagcatgccc gcgcacagat agttacaggc
     4441 acgccagtag gcgtccatcc tccgaagttc atcggaagtc aatggcgtaa gttcgaaagg
     4501 agaggcgttg gtcgtagcgg aagaaacggg gatgctactc aagcggtcac ctcgtgcaag
     4561 ggaatgggtt ggtcctcgat cgcgttcatc ccttgatgga tcgagttgca gagtgcgatt
     4621 gcatgccaat tatgagatgt tcctgttaag cgacggtgaa cacagccttc cccgacttgg
     4681 ttgagccaat tcggcggcgc ctagatcgtg gcttcctcat ggatgtcacg ctttgccctc
     4741 gggatgcacg atacgacatt tgccggactc catcgcaggc atccgcgggt cacgttttgc
     4801 gtcaagagac atcctgacac agcgcctcgc cgattgctgt accgaattgc acagcgtgtc
     4861 atcttatgag gtgggacact gcaaacgcag gcgctcttag cagcccgctc agccctatat
     4921 gaatattaag ttcgacacag gagaattcca tgccacagag cccgcacgat cgcgccgcag
     4981 aactacacaa ccttgcagcc cacgcccatg ccgcagccgc taccgcccac ggcaaaggag
     5041 atcacctgac cgctcacgag ctaagcaaaa aggcgcacga gcactccatg aacgcgttca
     5101 aacacgctaa cgaggcattc ccggctgccg ccgggggcaa aacaccatag ggcgcagagg
     5161 cttgctggta cgatacttcc taaaagagaa agcccggcat gaacgtcttt gtactcaatt
     5221 ccggtagttc cagcctgaag tttcagatca tcgccactga tctggatcgt atccggcagc
     5281 atcaggatga acgaatctgc cgcggcgagg tggaacgcat cggcggcgaa gcgattgtca
     5341 cgttccagaa gggcggtgac gcaaaacaga agttcactgc gccgttgcga gacatagcgg
     5401 cggcgctgga ttacctggtc cgttacattg cctccgacaa atcgggagtc actgagatca
     5461 agagttcagg tgatattcac gcggttggac atcgcgttgt gcacggcggc gagatgttct
     5521 cggagtcggc ccttatcgat agcaaagtgt tacaggatat tgaagactgt atcgatcttg
     5581 ctccgcttca caatccgaac aacatcaagg gcatcgtggc tgcgcgagag ctcttcggca
     5641 aggacgttcc gcaggtggcc gtgttcgata cttcatttca tcattcgatc ccggagcaag
     5701 cattcctgta cgccctgccg tatcacctct atcggcgtca ccgcatccgc cgctacggat
     5761 tccatggaac ctcgcatcgg tacgttgcgt ttcgctaccg cgcgttgcgc gaattgccgc
     5821 gcgagcagac caacatcatc accctgcatc ttggaaacgg ctgctcggca accgcgatcc
     5881 gaaaaggaaa ttcggtggac acctccatgg gcatgacgcc tcttgaaggc ctggtcatgg
     5941 gtacgcgctc gggagatctc gatccggcga ttgtgaatct catagcaacg aaagaaggca
     6001 tgtcaccgtc agaggtcgat gcgcttctga acacgcaatc gggtttgctg ggtatttccg
     6061 gtctgtccaa cgacatgcgg atgctgcagg ctgagctcca agaaaaccac gaccgtcgcg
     6121 tgcaactcgc gattgaagtc ttctgctatc gcgcacgaaa gtacatcggc gcattcctcg
     6181 ctgccatggg tggtgccgac gcggtggtat tcaccggcgg catcggagaa aactcgcccg
     6241 agattcgcgc gcacatttgc gaaggcatgg aatgggccgg ccttacgatc gatccagtga
     6301 agaaccagca gacggttggc cgcgagggcc tcatcagcac cgaggattcc aagctggtcg
     6361 cctatgccat tcccacggat gaggaattgc tgatcgctcg cgataccgtc cgggtcatac
     6421 tcggcgagcc acatccctcc tgatgcgctt cgatttcgcg ggggccaagc tctagttctt
     6481 tgataggcgg gatcaacggc tgtcgcaccg ccgccgatgg ccgggcaatc tactcctgac
     6541 gcacgctatc gcgggatagc ccgacagtta cgagaaggga cagggatggg aaaaccagca
     6601 acctctgacg agagcttttg ggaccggccg ctgcacgaac tgttcgaact attgccagcc
     6661 actccagcag gactaacgac cgaagaggcc cggcggcgat tgcgtcttta cggcccaaac
     6721 agctttgtcc atgagtcccg cttcgctgcg ctcctcagtt tttttggttt cttccgtaat
     6781 ccgctcgtcg tcattcttgt tatcgccagc ggcgtatctc ttgcgctcgg agagcatgtc
     6841 ggcgggttaa tcatccttgc gatcgtatta ttcagcgtaa tcctcaattt cctgatggaa
     6901 ttccaggccc ggcatgccgt ggaggacatt cagaaacaga ttgcgctgac tgcagcggtg
     6961 gtgcgcgatg ggcgagagca ggaactgccg accgccgagc tggtcccggg agacatcgtc
     7021 cgcctcaatg caggagacct ggtaccagcc gacgcccgtt tgctggaggt gaaggacctg
     7081 catgtgcgcg aatcagtcct cacaggggag tctctgccag tcgaaaagac ggtggctgac
     7141 cttcccaagg cgaagcacag cgttgcggat gccagcaaca gcgttttcct gggcaccgcg
     7201 gttcagacgg ggattggcac cgcggtcatc gtctgcactg ggaaagacac cgcctgcggt
     7261 gagattgcca atcgtctcgc tatgcggccg ccggagaccg agttcgggcg cggcatacgt
     7321 catttcggga tgatgctcac atgggtgacc atgttgctcg ttcttttcgt cctgttggtg
     7381 aacatcattt tccatcgtcc ggtgcttgaa tctttcctct tctccgtcgc gctcgcggtc
     7441 gggatgacgc ctgaaatgat gcccatgatc atcacggtaa cgctggccca gggcgcgaag
     7501 cggatgacga agaagaaggt tctggtcaag caactgtcgg caattgagga tttcggcagc
     7561 atcgatatcc tatgtactga caaaacgggc acgctcactg agggcgagat cgtgcttgac
     7621 cggcacgtgg atttccaggg taaggacaac gataacgtgc tgcagctcat ctacctgaac
     7681 agccactttg aggcgggcat caagagccct cttgacgacg cgatcttgaa gcacgaggcg
     7741 ccttccatcg agggatatga caaggtggac gagattccat tcgacttcaa ccgcaagcgc
     7801 ctttccgtgg tggtgcggca cacggacgaa gaccttctga tcaccaaggg tgaggcggaa
     7861 agcgtatttg ccatttgcga gacggtgacg gtcgacggcg tcgctcagcc tttcgacgag
     7921 aaacggcggg cagaggcgga agacacgcta aagaagctga gtgcggatgg attccgcgtg
     7981 cttggagtgg caactctcaa ggctcaaaaa caggatgcat acgccgtggc cgccgaacac
     8041 gcgatgacac tggtcggttt cgcggcattt ctggatccac cgaaagaggg agtgcttgcc
     8101 gtgctgcagg ccctgaaaca gaacggcatt tctgtggttg tcatgacggg cgacaaccag
     8161 tacgtaaccc agaaaatagc ccacgacgtg gggttggata cggaccacat tcttgtcggc
     8221 aatcagctcg atacgatgga cgatgccgcc gtggcccacc aggcggaaaa cggcgccatc
     8281 ttcgcccggg tgtcccccga acaaaagaac cgggtaatac tcgctttgaa ggcccgcggc
     8341 cacgttgtcg gatacatggg cgatggcatt aacgatgctc cgtcactgca cactgcggat
     8401 gttgggatct cagtcatgaa tggcgtgaac gtagccaaag acgcggccaa gatcattctg
     8461 ttggaaaagg atcttgcggt cataaacgag ggagtgctgg aggggcgtcg ctcctttgcc
     8521 aacatcatga agtacatcat catgggcacg agctcgaact tcggaaacat gttcagcatg
     8581 gctgcggcat cgctcttcct gcccttcctg ccgatgctcc ccagccagat cctgctgaac
     8641 aattttctct acgatgtttc gcaggtcagc atcccaaccg acaacgttga tcccgccatg
     8701 gtgcaaaagc ccaagcgatg gcagattggc ttcatccgcc agttcatggc gatcatcggt
     8761 ccaatcagtt caatctatga cttcctcact ttcggagtgc tgctctgggc gttccatgct
     8821 tccacgaacg agtcgctctt ccacaccgga tggttcgtag agtccctcgc tacacagacg
     8881 ctcgtggtgt ttgtgatccg gacagccgcc aaccccctca agagccgacc cagccggccg
     8941 ttggtgatta gcgtattggc cattgtgact gttgcgaccg tgctgccata cactccgctg
     9001 gggaccttgc tgcggtttac gccattgcca gtatcgttgt tggtggcaat tgcgtttctt
     9061 gcggtaacgt atctgttcgt cgtgcaggcc gtgaagtcct ggttctatcg ccggcacgcg
     9121 ctgctgtgat ggcgccagtt gaggaagaca cagtaccggg ctgactcccg actgtacggg
     9181 atcatgcaac tgttccgaat acccatccga tgcccgaggt gatcgccatg gcgagcgcac
     9241 cccagaaggt gacgcggata gcgccggtca tcactctcgc gccgcccgca tgagccgcca
     9301 gcccgcctag aagcgcgagg aacagcagtg aggtcccggc cacaagaggg atcagggccg
     9361 cctcgggagc cacggcagta accaggaggg gcagaactcc tccgatggcg aagctgcctg
     9421 ccgatgccaa tgccgcctgt atgggacgcg cgcggagagt ctcattgatc ccgagttcgt
     9481 cgcgggcatg cgcgcccagc gcatcgtggt ccatcaactg ctcggccacc tgcttcgcga
     9541 gcgcaggttt gagtccgcgc ttgacataga ttgcttgcag ttccttgtgc tcgccgttgt
     9601 cgtctgcctt gagttctgcg cgttcgagct tgaggtcggc ctgttcggtg tcggcctggg
     9661 aatggacaga cacatactca ccggcagcca ttgacatagc tcccgctacc aagccggcaa
     9721 ctcctgcgac cagtacattg cggtgattcg catgcgcggc ggcgacacct agcaccagac
     9781 tcgaggtgga aaggatgcca tcgttcgcac ccagtaccgc cgcgcgcagc cagccgatac
     9841 gttgtgtgcg atgtcgttcg acgttgcctg cggccatgct tgaggctttc ctgagttgga
     9901 aactgtctaa tgcgaaacga aatatgattg aggcaccctg tttcgacgac acccggaatc
     9961 gaactcccgc catgctctac ttgggcgcgt tcaattccag tgcccttgag ggcttggaga
    10021 gaggcggcct gtgggcacgc tctttctcag gcggttcccc gggctcggca ggtggttcta
    10081 gggccttctt gccgttggcg gtcagtttcg cggctcctgg aatggctgtc ccaccgtcat
    10141 cattcagcac aacgctcagc agtgaactgt ggacacggag gtgatgtcca gtgtcatagt
    10201 tgatgaagcc gagctttctg aatttgttca tgaagaagct aacccgtgag cgggtcgtcc
    10261 ccaccatctc ggccagagtt tcctggctca gcttgggaac cgctatttcg gtcaccactc
    10321 cctctttgcc aaagtgcgcc atcaacaaga gaatgcgcgc cagccgtttt tcgctggagt
    10381 tgaatagctg atcgatcaga tcatcctgat agcgaacgtt tcgcttcagg agatacttga
    10441 cgaaggtggc cgataacttc ggttcgcgac tcatggccag catcattgcc ttcttctcga
    10501 cgtgcagaag gacgcaatcc gtcatggcgg ttgccgacga catgcgcacc aactggccag
    10561 caagaccacc ttctccaaag aaatcgtcgt ctcctaaaat gccaagcgtc gcttccttcc
    10621 cggcttccga tacaacgctg agctgcacct tcccttcctg gataaaaaac agcccgtcgg
    10681 aaggctcgcc ttgcgcaaag atcacgtggt tcttttgaaa cggcatcata cgtctgccgc
    10741 tcccaatgga tgtcaggaag gtacgcgggt tgaaatttcg ttgtgctatc gcgttcgtca
    10801 tttgcttcac cgagattccg tctaagcgag gggaagtgtc ctcaagctct attatcggaa
    10861 gacagaggaa gctccgctgc tcaatagcgc tcacaaacaa gcactcgtga aaaagccgca
    10921 tctgcgttcg tttccaaact aaatagcgac cctgagacgc tcagttgcct cattcacggc
    10981 cgcctctctg gatgcgacga ttcgaatcca gcgaattagt gttgtcggac cttaagctgc
    11041 cgtcacgggt ttcgacgatt gcgtggcatg aatcagggcc atctcgacca ggtgctcgct
    11101 cagaaaccag ggcccagcgc actgggccct tctctcaagt attcgattgg ttgttacagc
    11161 tacctccgct tcaccatcca gccaatcaac attccgacgg ctacgcctgc ggtgaatgtc
    11221 actgcaactg tctcaatggg atgtcgctgc aggcgtttgg ttgtgtcgtc catgaactcc
    11281 tcagcagcat cgccgctttg ctttgcggca cgcttggctg cttctacgcc gtcttcgaac
    11341 gcatctgcaa cggcggatgt cgcccgagac gccttgcgcg ctgtttctgc aatttgctcg
    11401 cccgttcgtt ccaatactga ttgtgtcatt gttgtattgc cctctctcgt gtgctggttc
    11461 gatgttggtc ctgcttattc cccgacaagg ttttcaacct tgcctaccca gttctgggct
    11521 ttgccagcat tcttctcggc tctgccgtca gcttccaggt ctgggttgtt gataagttct
    11581 ccggcctttt ccttgattgt tcctttggct tcgtggagtt tgcctgttgt cttgtcttcc
    11641 gtactcggtt tcataacgta tatctcctca gtttggtagt aagggtgaat caccactcat
    11701 cactcgaagg tcacagcctg ccgcgttatg ccactttctt atcgccaggt gtctgcacct
    11761 gaaccagttc gtcgaagagc gagcgatagt ggatcatcgc ggtgcgcaac tcttccgtgc
    11821 tggcatcgcc agcgcccaaa cgcaaagcga tgttgtgtgc tgagcggtaa ttctccacca
    11881 cgcgaggatg gtcaacggaa atatccgccg ctcgctgatc gaaatcagtg acaggataac
    11941 cgcgagtctg catcagcgaa cacaccaact catcggcttc agtcaccgca ccctttggaa
    12001 aatcgacgaa gcgggattgc accgtctgcc acagtgccag gtagcgctca cgctccgtca
    12061 ggtcgagatc gcggatattc aaccgctcaa cccgcgtttc gcggtccgcc agcttcgctt
    12121 cagctttacg ctcagaacca tgttgcttga cggcgcgatc gtattccggc ccgaaacggt
    12181 ttttcagccc ggtcgtagta ttgctgcgtt ttcgcatata cagtgccacg gcgacaacga
    12241 ttaccactgc caatacgact gcgaaggcaa tcagtttggg atccattaga ttgagattca
    12301 ttcgggaact ccttaaatca ttcgcgtctg cgaacagttg ggatcaggat tggaaaatct
    12361 tcttgcctga ggcagagtat ccaacgatcg atctgccgaa agctggtcag tattgatcgt
    12421 ccagccgctt agagtacaga gtgggcatgt tgctcgcccg gcgaagtaac ggcgaatcat
    12481 gaaagtcggc gctgcccaaa gcatgatccg acattatctt gagcacgccg atcctgtgcg
    12541 tcactgaatg tggcggaccg cgtccgctgg cgctcctatt ccgatggagg tttctgttcc
    12601 ttcttttgct actccgcagg gtctttctga cgcgtcgctt tgacccccga aagaattccc
    12661 actcccaaca gcacgaccgc gcccaccgca atccagtgag tcggtatgtg catcaaatgt
    12721 gcgccataaa tcagcccggc gataacaatc gcgaacccga tcgaatacag tccgaatgac
    12781 ataacaactc cttctcgatc ctgtgctatc tgttttcgct agtgaaagcg gccaactcat
    12841 tccccagcga gcttccggaa ccgcggccgc tctgcgtcat ctgcgcactg tcgaatccac
    12901 aaatcctggc gccgggaata aggtcgagag taactccggg ggccgcaact caagaatcgg
    12961 gcgaaccctg tagtcatccg gcgaaatgtc cttactgacg agtcacgtca catggtagcc
    13021 accggaaagc ttcagttctg tgcagaaacg accacattgc gatcgaccag cgacacccca
    13081 tcaacggtca aatgtgcaca gcattagtta cgagcatcct tgaggatgac agttagaaga
    13141 tccttaaagg agattcatcg tgaaaagtac ggcaatgttt ctaccatgcc tcatggtatt
    13201 aggcgcgtcg gtgctggcaa tgccaccccc gacgcaggcg caatcatccg atccaggcac
    13261 gaagcagaca acccaggtag ttacgaccac aacccggaca acaaccttcc acatcaccgt
    13321 ggtgagccgg acggtgcagg cagtcaatta tcgccatcgc ggtggagcga ccaagttgga
    13381 tttcgcagga accaacctga tgcctgctgc cacgggcgag gccaaggtgg agagcaagaa
    13441 aggctacatc gaaatcgaag tcgaatttgc caggctggca agacccacta cctttggcaa
    13501 tgagtatctt acttatgtaa tgtgggcgat tagtcctgag ggccgcgccg tcaacctcgg
    13561 agaggtattg gttggagaca acgagcgaag caaattggac gtcaccaccg acctgcaggc
    13621 cttcgcgctg atcgttacag cggagccgta ttatgcagta cgccagccca gcaatgtcgt
    13681 cgtgcttgag aatatcgttc gtgaagacac caagggaact tccgaagtcg tgaaggcaaa
    13741 gtacgaactt ctcgagcgtg gtgggtacat tcccaccggc tacacactag acccggtcgt
    13801 actgaacgcc aacctgccgt tagagttctt cgaggcgcgt aatgctctgc gcatcgcgca
    13861 gtccgtcggt gcggatagat acgctgtcgc cagttaccaa catgctgtca gcctgatgaa
    13921 caaggcggac gcatacgcga ccagcaagca catcgacaag aaagaactga tttccgtctc
    13981 acgcgaagcc gtacagacgg ccgaagacgc gagagagatc gcagtcaaga agctgattgc
    14041 cgacaaccaa tccaacgaat tgcaggcgtc agcccgggct gccaacgacg caacccggca
    14101 gagagatctc gcgacggcgc aggcagccaa agcgaaggcg gacatggagc gcggccaagc
    14161 cgattcagcc aatcaagtgg cggcggcgca tgccgaaacg gagcgcgctc ggctggctac
    14221 tgcccaagcc aacgtgcagg tacaagcagc tgaaaacgac aaagtcgcga tgcgtgaaag
    14281 actgtcgcta cagttgaaca ccatcctgca aactcgcgac accgcacgcg ggttgatcgt
    14341 gaatatgtcc gatgtcctgt ttgacactgg caagtacacg ttgctgccgg gcgcccggga
    14401 gaaactctcc aaggtcgctg gcatcctgat cgcatatccc ggcctcaata ttgcggttgg
    14461 gggatacacc gacaatgtag gcagcgatca gttcaaccaa ggtctgtctg agcatcgcgc
    14521 ctcgtcggtt cgcgactatc tcgtgcagca aggcgtattg gcgaattcca ttacatcaaa
    14581 tggctttggc aatacgatgc cagtggcttc gaacgataac tccgccggac ggcaggagaa
    14641 ccgacgggtt gaactcgtcg tatcgggcga gtcgattggg accgatgtca acgtcacgac
    14701 tggaagcttg cagtaaccag caactgtcgg cttgctcata gcgcgattcc aaccaggatc
    14761 gcgctatctt ttgctggccc ggaaccaagt ctgctctcgg ccgtgtgctt tgcgcgcaga
    14821 gttgtgcggg ctaactaaag cttgcgaaaa gggctcacgc catcactgag tcagccgaaa
    14881 tgctccgtgg agagccgcag tcgagtcttc gcctatccac acgtcaaact gctccggctc
    14941 aaccacccac tgtttctttg tgggactcca gaactgtaat tcttcgggac cgagttggaa
    15001 gtgcacggtt cgtttcgcgc cgggctcgag ggtaacgcgc tcgaatcctt tcaattgccg
    15061 caccggacgt gaagcgctgc ccgcccgctg gtgaatataa atctggacga ccgtgtcgcc
    15121 tgcccgactg cccgtgttcc tcacatctgc tgagacctca acatttgcgc cgactttcgc
    15181 ctcgacccgg gaaactttaa ggttatcgaa cgcaaatgtg gtgtaactga ggccgtaacc
    15241 gaagggatag agtggcgtgc tgagtacgtc ccagtagcgc gatttgaaat ccggggatgt
    15301 ttcgtgcagt tgcgtcagat tgtggttgta gtagatcgga acctgtccgg tactgcgcgg
    15361 ccaactgact ggcaagtgtc cggcgggaga agcgtcaccg aacagcacat cggcaattgc
    15421 gttgccgccc tggctgccgg gataccacgc ctccagaatc gcaggcacat gttcggctgc
    15481 ccacgagata tccaatggcc gcccattcac cagcaccagc acgacgggct tgcccagcgc
    15541 cgaggccgct tccagcatct cctgttgtcc gttcgacagc cgaagcgaag agcgtgaagc
    15601 cgactcggcg ctcatcagat caagctctcc cagcaccacc accgtaatgt cgctttgctt
    15661 ggcgagggca actgcttcgt cgatcgcctt gcgagcctcc tccgaggctt gctgcggctg
    15721 ctccttcatc ggggaaatcg gaatggtttc gaatggcgac gggaaatcac gccggaggtt
    15781 gggcccgtgc gcaaagtcca cgcgtaccgc agtgccaacc ttgtcccgaa tgccctgcag
    15841 gacgctgacg gtcggacccg gcttcgtcat atggccccac atgctcagca agtcgtcctc
    15901 agcatcggca agaggaccga tcgccgcaat agacttaaga ttgctacggt cgagcgggag
    15961 caggttcttg ttattgcgta gcagcaccac ggaacgctgc gccgcgatgc gcgccagttg
    16021 gcggtgttgc ggatcgttca gtacctgagc gctgcgcgat tcgtcagaat aagggtgctc
    16081 gaacaacccc atgcggatct tgacttcgag aatcgagcgc acagcgttgt cgatctgctg
    16141 catagagatt cttccctgct ggacttcctc gagcagaaac ttccagtagg ttccgctggc
    16201 catgtccatg tctagcccgg ccgaaaaggc cttgtaggcg gcgtcctggg aatcgcgggc
    16261 atagccatgc gtgaccaggc tgtgaacggc gactgcgtcg ctcacgacaa agccctcgaa
    16321 actccacgcc cggcgcagga cgtcagtgag caaccacttg ttaccgctcg cgggcacgtc
    16381 gttcaggtcc atgtaggcgc tcatcaggct gccgacgccg gcatcgaccg cggccttgaa
    16441 cggcggcaga tagacgttcc acatcaactc ctcaggaatg taggaggagt cgtagtcgcg
    16501 gccgccgtcg gctgcac
//
LOCUS       DASRKK010000447         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_621300, whole genome shotgun sequence.
ACCESSION   DASRKK010000447 DASRKK010000000
VERSION     DASRKK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621300"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFC15_10520"
     CDS             <1..321
                     /locus_tag="VFC15_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10633.1"
                     /translation="QRANQCFKYREGIFFREQRSGRGISRFVSAKRVLGSGDLLVEGY
                     AVPGVPKHAERVPRSKDRRAAGAVDHLLFEFRAMASRSSDLMATALGLVIARELQSKK
                     KGNR"
     gene            321..1259
                     /locus_tag="VFC15_10525"
     CDS             321..1259
                     /locus_tag="VFC15_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZM10634.1"
                     /translation="MPATFRRAGVAIFVVAVLPLPVAAQNLTAAEAILRIQQRYSTTL
                     PPGTVDTVKAGDPSTPVTGIATTFLDTMDVLREASRRGANLVITHEPTFYNHLDDTAF
                     FADDPVYREKLAFIQQHHMVVFRLHDGIHASSPDHIAIGLVKSLGWQGYTDAGDPFTL
                     TIPRTTLAKLTTELAGKLKIQTLRVVGDPNLAITRVAILPGASGLKKQVLALRRDDVE
                     VLIAGESSEWEAVEYARDASAQGRHKALILLGHESSEEPGMEQCTEDLRALFPKLLVI
                     HISAGQPLWSPEHPPTSASAKASDKYGHLLRQKGSP"
     gene            1586..2002
                     /locus_tag="VFC15_10530"
     CDS             1586..2002
                     /locus_tag="VFC15_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM10635.1"
                     /translation="MKRYLLDTNVVSELRKHKPHGAVVNWLTNLQEDQIFLSAVTLGE
                     LQAGIELTRRQDPIKAREIEHWADQLEASYQVLPMDSATFREWGRMMHGKSDHLIEDG
                     MIAATARTQGLIVATRNERDFKQLNVAVFNPFHVKA"
     gene            complement(2051..2127)
                     /locus_tag="VFC15_10535"
     tRNA            complement(2051..2127)
                     /locus_tag="VFC15_10535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2091..2093),aa:Arg,seq:tct)
     gene            complement(2185..2754)
                     /locus_tag="VFC15_10540"
     CDS             complement(2185..2754)
                     /locus_tag="VFC15_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10636.1"
                     /translation="MDADWSVELGHDDPSLEFPWASPDGTQRFVDLSRHLEAICEIPE
                     AAQYPELARFLLELNGKFSPWLTAKCDVWIDNELGEAEDIYDAKLKMCSYIDLVRRNE
                     SERFSFEQHEQWVKSTAMTLRNIGPHPIGCELIVRRCWYPTGSRADDDPVPGFYITLY
                     VSGYGDNEAEALARWAGGLTRVSSIVLGV"
     gene            complement(2754..>3527)
                     /locus_tag="VFC15_10545"
     CDS             complement(2754..>3527)
                     /locus_tag="VFC15_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM10637.1"
                     /translation="TGSKAWRGGMNVVRMNMRNCGGTEQLGPTLYNSGMSDDVGLVAE
                     TLIREEVLQKVAFAGFSMGGNLVLKLLGEWGAQAPPQVKAGIGVSPAMDLAASADALH
                     DRANRLYEWRFLRGLKKRIERKAALYPGRYDTRYLQGLGSIRDFDDQITARYCGFRDA
                     QDYYTRAAAAQVLDRIAVPTLVLHAEDDPFIRVLPDTLEKLQKNSHITYLETEHGGHC
                     AFLAQPNGYDGRWAERQAIAFVQRVERGEPVTAPAWAQP"
BASE COUNT          732 a         1050 c         1016 g          729 t
ORIGIN      
        1 cagcgcgcaa accaatgctt caagtatcga gaaggaattt tcttccgcga acaacgctcc
       61 ggcaggggca tttcgcgttt cgtgtccgca aaacgagttc tcggcagtgg agatttgctt
      121 gtcgaaggat atgcagtacc aggcgtgccc aagcacgctg aaagagtgcc acgctccaaa
      181 gatcgacgtg cggccggtgc cgtagatcat ttgctgttcg agtttcgcgc aatggcgtca
      241 cgctcttcag acttgatggc gactgcgttg ggacttgtca tcgcccgaga attgcagtcc
      301 aaaaagaagg gaaaccgttg atgcccgcca ccttccgtcg cgccggtgtc gccattttcg
      361 tagtcgcagt tcttccgctc cctgttgcag cccaaaacct caccgctgcc gaggcgatcc
      421 tgcgcatcca gcagcggtac tccactacgc tgccgcccgg cacggttgat accgtcaagg
      481 caggcgatcc ttcaaccccg gttacgggaa tcgccaccac cttcctcgat accatggatg
      541 ttttacgaga agcttcccgc cgcggcgcca atctcgtgat cacgcacgag cccacttttt
      601 acaaccatct tgacgacaca gcctttttcg ccgatgaccc cgtttaccgt gagaagctcg
      661 cgttcattca acagcaccac atggtcgtat tccgcctgca cgacggaatt cacgcctcct
      721 cgccggacca catcgccatt gggcttgtta agtccctcgg atggcagggg tacacagatg
      781 cgggcgatcc attcacgttg acgattccgc ggacgacgct tgccaaactg accactgaac
      841 ttgccgggaa acttaagatt cagactctcc gcgtggtggg agatccgaat ctcgcgatca
      901 cgcgcgtagc catcctcccc ggcgcctctg gtctgaagaa gcaggttctc gccctccgcc
      961 gcgatgacgt cgaggtactc atcgcgggcg aatcctccga gtgggaggcg gttgagtacg
     1021 cgcgcgatgc cagcgcgcag ggccgccaca aagccttaat acttttggga cacgagtcct
     1081 ctgaggaacc tggcatggag cagtgtactg aagatcttcg cgccctcttt cccaagttgc
     1141 tggtcataca catctcagcc ggccaacccc tatggagccc ggaacacccg cccacttctg
     1201 cttcggccaa ggcgtcggat aagtatggac atctgctgcg gcaaaagggg agcccctagg
     1261 agctgttcaa ctgatacaat tgactagtct tctgactagt tcgttctctt gttgagaatt
     1321 ggaggtatcc cgtggccgca tggcaagtac aagatgcaaa ggcccgcttc agcgagttgc
     1381 tggacacgac gctcaagaag ggaccacaag tcgttacccg aaggggcgta gaggccgcag
     1441 tattggtgcc gatcgaagaa tggcatcggc tgcagaggtc gtcgcgccct ggcctgaagg
     1501 cactacttct cggttctggt ccgcgctttg agaggctggt tcctgaacga ggcggacgac
     1561 gcaggcggga gcctgtgaag ttctgatgaa gcgctatcta ttagatacca acgtagtttc
     1621 ggagttgcga aagcataagc ctcacggtgc ggttgtcaac tggctaacga atttgcagga
     1681 agaccagata ttcctgtcag cagtgaccct gggtgaattg caggcaggaa tcgaattgac
     1741 gcggcgccag gacccgatca aggcccgcga gatcgagcac tgggctgatc aactggaagc
     1801 ctcgtaccag gttctcccca tggattcagc tacctttcgc gaatggggcc gaatgatgca
     1861 tggcaagtca gatcacctga tcgaggatgg aatgattgcc gccacggccc gcacgcaagg
     1921 cctgatcgtg gcgacgagga atgagcgcga tttcaagcaa ctgaatgttg cggtgttcaa
     1981 tcccttccat gtgaaggctt gagctaaccg gcagccggta acgcggcagt cgtgctgatt
     2041 cggggagtga tggtagggcc ggctgggatc gaaccagcaa ccctcagctt agaaggctga
     2101 tgctctatcc aattgagcta cggccccatc gtggcaggac gcaagaaatc ggcctgccga
     2161 attcattatc gcaaatcagt cccgctacac gccaagtact atggaagaga cccgcgtgag
     2221 gcctccggcc cacctcgcca gcgcctcggc ttcgttgtcg ccgtacccag acacatacag
     2281 cgtgatgtag aagcccggta caggatcgtc atcggcgcgc gaacctgtgg gataccagca
     2341 gcgtcgcacg atcaactcgc aaccgatcgg gtgcggaccg atgttacgca acgtcattgc
     2401 ggtcgatttc acccactgct cgtgctgctc aaaagaaaag cgctctgatt cgttgcggcg
     2461 gaccaagtcg atatacgagc acatttttag cttagcgtcg tagatatcct ccgcttcgcc
     2521 caactcattg tcgatccata cgtcgcactt cgcggtcagc catggggaga atttgccgtt
     2581 gagttcgagc agaaatcttg caagctcagg gtattgtgcc gcctccggaa tttcacagat
     2641 cgcttcgaga tgccgcgaca aatccacgaa gcgttgagtg ccatcgggcg acgcccaggg
     2701 aaattccagc gagggatcgt catgacccag ttcgaccgac cagtcggcgt ccatcagggc
     2761 tgcgcccacg cgggagcggt cactggctct ccccgctcaa ccctctgtac gaacgcgatg
     2821 gcctggcgtt cggcccaacg gccatcgtag ccgttaggct gggcgaggaa cgcgcagtgc
     2881 ccgccatgct cggtctcgag gtaagtgatg tgtgaattct tctgtagctt ttcgagtgtg
     2941 tcgggcaaca cgcgaatgaa cgggtcgtcc tcggcgtgca ataccagtgt gggtaccgcg
     3001 atgcgatcga gcacctgcgc cgcggctgcg cgggtgtagt agtcctgggc atctcgaaag
     3061 ccgcagtagc gcgccgtgat ctggtcgtcg aaatcgcgta tgctgcccag gccttgcaga
     3121 tagcgagtgt cgtagcgtcc tggatacaga gctgccttgc gctcgatacg tttcttcagg
     3181 ccgcgcagaa acctccactc gtagagtcgg ttggcccgat catgcagcgc gtcggccgag
     3241 gccgcaagat ccatcgcggg cgagaccccg atgccagcct tgacttgcgg aggggcctga
     3301 gcgccccatt cgccgagcag tttcagtacc aggttgccgc ccatagagaa tcccgcgaag
     3361 gccaccttct gcaagacttc ttctcgaatc agggtctcgg ctacgagtcc cacatcgtca
     3421 gacatgccgg agttgtacag cgtggggccg agttgctcgg tgcctccgca attgcgcatg
     3481 ttcatgcgca ccacgttcat gccgccgcgc caggccttgc tgccggt
//
LOCUS       DASRKK010000448         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1404422, whole genome shotgun sequence.
ACCESSION   DASRKK010000448 DASRKK010000000
VERSION     DASRKK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404422"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2360)
                     /locus_tag="VFC15_10550"
     CDS             complement(<1..>2360)
                     /locus_tag="VFC15_10550"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM10638.1"
                     /translation="EMAFQYKLVSAVVVVVFCLAVCAISGSAQTTGNSSLSGVVADPS
                     GAVVPGATVEIHNPVSQFDRSTTTDGAGRFSFPNVPFNPYHLRVNVTGFAAYSQDIDV
                     RSAVPLSLKITLQIAGSAESVTVQGEASDLLENDPTFHTDVDRSLFDKLPLESSSSEL
                     SSLVTLASPGVAADSNGLFHGMGDHAENSFSLDGQPITDQQSKVFSNQIPLDAVQSMT
                     VIGGAPPAEFGDKTSLVIDVSTRSGLGSTTPHGGITASYGTFGSSNLDLNFSYGGKSW
                     GNFIAANGLNSGRFLDPPEFVVFHAHGNEENVFDRFDYQFSPADALHFNFGFTRSWFQ
                     NPNSYDNLNIGVVGPNGLPVGPTDQRSKILTFNIAPTWTHTLSPNAVLNVTGYVRRDA
                     FNYYPSNDPFADLGPPSLQRETVAQQRTLLNAGVLANVTYTKGIHNVKAGVMYEQTLL
                     NENDQFGIVDPTLNAPCLNGNAVPVNGFTDPSQCAGAGYQPNVASNPNAPNSALYPLF
                     NGVLLPYDLTRGGGLYPFIGHTDVKELALYVQDNINVRNWSFNLGIRGDLYNGLTTAS
                     QAEPRVGIAYNIQKTNTILRASYARTLETPFNENLVLSSIGCNSPVLNPLLGCTSSRL
                     TPLAPGFRNEFHAGLQQAFGRFLVVDGEYIWKYTHTGYDFSVFGNTPIFFPIGWHNSK
                     IPGWALRASVPVYHGFSALVVMSSVAARFFTPQISGAGANPLAPQGVFRIDHDEAYNM
                     TAHLQYQTPWKRGPWLGFNWRYDSGLVAGAVPCYGIGPTNDCPQST"
BASE COUNT          503 a          640 c          741 g          476 t
ORIGIN      
        1 tggtcgattg cgggcaatcg ttggttgggc cgattccgta acatggtacg gccccagcga
       61 cgagtccgct gtcgtagcgc cagttgaagc ccaaccacgg gccgcgcttc cagggggtct
      121 ggtattgcag gtgtgcggtc atgttgtagg cttcgtcgtg gtcaatgcgg aaaaccccct
      181 ggggcgccaa gggattggct ccggctccag aaatctgggg agtgaagaac cgggcagcaa
      241 cgctggacat caccaccagc gcgctgaagc catggtacac cggtacgctg gcgcgcaacg
      301 cccaaccggg aatctttgag ttatgccacc cgatcgggaa gaaaatgggg gtgttgccga
      361 atacactgaa atcgtacccg gtgtgagtgt atttccaaat gtattcgcca tcgaccacaa
      421 ggaatcgccc aaatgcctgc tgaaggccag cgtggaactc attgcgaaag ccgggagcca
      481 atggcgtcaa cctgcttgat gtgcatccta gaagaggatt caggacgggt gagttgcatc
      541 cgatgctgga gagcaccagg ttctcgttga aaggggtctc caatgtgcgc gcataggaag
      601 cgcggagaat cgtattcgtc ttctggatgt tgtaagcaat tcccacgcgc ggctccgcct
      661 ggctggcagt ggtaaggccg ttgtaaaggt caccgcgaat accgagattg aagctccagt
      721 tgcgcacatt gatgttgtcc tgcacgtaca gcgcgagctc tttcacgtcg gtgtggccga
      781 taaatgggta gagacctcct ccgcgcgtca ggtcataggg caacaggaca ccgttgaaca
      841 agggatacag ggcagagttg ggcgcgttcg ggttggaagc gacgtttggc tgatatccgg
      901 ctcctgcgca ctgcgatgga tcggtaaaac cattcaccgg gacggcattt ccatttaagc
      961 agggtgcatt gagcgttgga tcgacgatcc cgaactggtc attctcgttc aggagggtct
     1021 gctcatacat aaccccggcc ttcacgttgt gaatgccctt cgtataggtg acgttcgcga
     1081 gcacgccggc attgagaagc gtacgctgct gggcgacggt ttccctctgc agactcggtg
     1141 ggccaaggtc cgcgaacggg tcgttgctgg ggtagtagtt gaatgcgtcg cgccgcacgt
     1201 agcccgtaac attcaggacg gcgttcgggc ttaatgtgtg tgtccacgta ggcgcgatgt
     1261 tgaaggtgag gatcttggag cgctgatcgg tcggcccgac gggaagccca ttcgggccga
     1321 cgacgccgat gttcaggttg tcgtaggaat tgggattctg gaaccaggag cgcgtaaaac
     1381 caaagttgaa gtgcagcgcg tccgccggcg aaaactgata gtcgaagcgg tcgaacacgt
     1441 tctcttcgtt cccgtgcgcg tggaaaacga caaactccgg cggatcgagg aagcggccgc
     1501 tgttgagccc gttcgccgcg atgaaatttc cccagctctt gccgccgtaa gagaagttca
     1561 agtcgaggtt ggagctgcca aacgtgccat aggaggccgt gatgccgcca tgtggcgtcg
     1621 tggagccaag gcccgaacgc gtgcttacgt cgatcacgag gctggtcttg tctccgaact
     1681 cggcgggcgg cgctccgccg atgacagtca tcgactgcac ggcgtcgaga ggaatctggt
     1741 tagaaaacac tttgctctgc tgatcggtaa tcggctggcc atcgagagaa aacgagttct
     1801 cagcatggtc gcccataccg tgaaagagtc cattggaatc cgccgcgacg cccggcgagg
     1861 ccagagtgac cagcgaactc agctccgagg aagagctctc cagcggaagc ttgtcgaaca
     1921 gactgcggtc cacgtcggta tgaaacgtcg gatcgttttc caaaaggtcg ctcgcttcgc
     1981 cttgcaccgt gacgctttcg gcagacccgg cgatctgcag agtgattttg aggctaagcg
     2041 gcacagcgga acgcacatca atgtcctgcg aataagcagc gaacccggtc acgttgaccc
     2101 tgaggtggta aggattgaag ggaacgttcg ggaagctgaa tctccccgcg ccgtcggtgg
     2161 tggttgaccg gtcgaattgg ctaactgggt tatgaatctc cacggtcgca cccggcacca
     2221 ccgcgccgga cgggtcggca accacaccgc tcagcgatga gtttcccgtg gtctgcgcgc
     2281 ttccgctgat ggcgcagacc gccaaacaga acaccactac cacaaccgca ctgaccaatt
     2341 tgtactggaa agccatctcg
//
LOCUS       DASRKK010000449         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_230443, whole genome shotgun sequence.
ACCESSION   DASRKK010000449 DASRKK010000000
VERSION     DASRKK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230443"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFC15_10555"
     CDS             <1..680
                     /locus_tag="VFC15_10555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZM10639.1"
                     /translation="KTVLAVPEGFSQEKVTLMEALGAEVIRTPQADGMEGAIRYVKER
                     AAKTANSFVAGQFENQANPDFHYETTARELYEQMGGQIDAVVIGSGTGGTFTGVARYL
                     KERNPRALAIVIETQGSVLGGGPKGEHEVEGIGASFIPKTCDLSLADEVIAVSDRDAF
                     DMVKKLAAIEGVLAGSSAGAAVFGAIEIARRLGPGKRVVTIIPDSAERYLSKDIFHFN
                     EKAPSED"
     gene            870..2021
                     /locus_tag="VFC15_10560"
     CDS             870..2021
                     /locus_tag="VFC15_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZM10640.1"
                     /translation="MSKKNTAGFATRAIHVGNDPDPSTGAVAPPIFATSTYAQEELGK
                     TKGYDYSRAGNPTRTRLEQNLASLEGGIAAPVFASGMAAVGALCQMMKSGDHVVASNN
                     LYGGVPRLFDQVMVNFGLAFTYVDTTDPRNVERAIRKNTRYVYIETPTNPLMGLTDIA
                     AVSKVAHKRGCEVVVDNTFMSPYFQQPIKLGADMVLHSTTKFLNGHSDGLGGVLVCTT
                     KEQAEKLAFIQKAAGAIMSPFECWLVLRGVKTLAVRMKQHDESGRVMADYLSGHRKVK
                     QVFYPGLKSHPQYELACRQMSGFGSMITFETGSLGNAKRLLKRVRVCTMGESLGGVES
                     LISHPASMTHAAIGEKGRKAIGLTDGMVRISVGIEDIEDLIADLEQALG"
     gene            complement(2207..2485)
                     /locus_tag="VFC15_10565"
     CDS             complement(2207..2485)
                     /locus_tag="VFC15_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10641.1"
                     /translation="MRKIAFTLLMTLFLSCVAVGTDTYQTAKVVKWENCTYQQKKNKV
                     GQWVVYSILTDATTTYEVARKKETKPKMQPGDTVQLEVKGNKATVINA"
BASE COUNT          563 a          746 c          775 g          552 t
ORIGIN      
        1 ataagaccgt gctcgccgtg cccgaaggct tttcccagga gaaggtcaca ctcatggagg
       61 cgctcggcgc ggaagtgatt cgcacccctc aggcagacgg tatggagggc gccatccgct
      121 acgtgaaaga gcgcgccgcc aagacggcca actcgtttgt cgcgggccaa ttcgagaacc
      181 aggccaatcc tgacttccac tacgagacca cggcacgcga attgtacgag cagatgggtg
      241 ggcagattga cgccgtggtg atcggctccg gcaccggagg cacctttacc ggggtagcgc
      301 gttacctgaa ggaacgcaat ccgcgggcct tggcgatcgt gattgagacc cagggttcgg
      361 tattaggcgg agggccaaag ggcgagcacg aggtggaagg tattggcgcc agcttcattc
      421 ccaagacctg cgatctttca ctcgcggacg aggtgatcgc cgtcagcgat cgtgacgcgt
      481 tcgacatggt gaagaagctg gccgcgatcg agggtgttct ggcgggatcg agcgccggcg
      541 cagccgtgtt tggggccatc gaaatcgcgc gacgactagg cccgggcaag cgcgtggtaa
      601 cgatcattcc cgattccgca gagcggtatt tgtcgaaaga cattttccac ttcaacgaga
      661 aagctccgtc cgaggattaa cgcggattgt tcttgaacca cagagacaca gagacacagg
      721 gaagaagaag gttgtcacca cggaggacac ggagggcacg gagaagaaat aacaattgaa
      781 actccgtgat ctccgtgtcc tccgtggtga aaaaagaatt ctgttcttct ctgtgcctct
      841 gtgtctctgt ggtgattctt taggttctta tgtccaagaa aaacactgcc ggttttgcta
      901 ctcgcgccat tcatgtcggt aacgatcccg acccgtccac cggggctgtc gctcctccca
      961 tatttgccac ctccacttac gctcaagaag agttaggcaa gaccaagggc tacgattact
     1021 cgcgagccgg taatcccacc cgcacacgat tggagcaaaa cctcgcttcg ctcgagggcg
     1081 gcatcgccgc gccagtcttt gccagcggaa tggctgcggt cggcgcgctg tgccaaatga
     1141 tgaaatcggg tgaccacgtc gtcgcctcca acaatcttta cggtggcgtg ccccggctgt
     1201 tcgaccaggt aatggtgaac tttggtctcg catttaccta cgtggatacg actgacccgc
     1261 gcaatgtgga gcgcgccatc cgcaagaaca cgcgctacgt gtacatcgag acaccaacca
     1321 atccgctgat gggtctcacc gacatcgcgg cggtcagcaa ggtcgcgcac aagcgcggct
     1381 gcgaggtggt cgtggacaac accttcatgt cgccgtactt tcagcagccg atcaagctgg
     1441 gcgcggacat ggtactgcac tcgaccacga agttcctcaa cgggcacagc gacgggctgg
     1501 gcggcgtcct ggtgtgcacc acaaaggaac aggcggaaaa gctggcgttc attcaaaagg
     1561 ctgcaggcgc catcatgtcg ccgttcgagt gttggctggt gctgcgcggg gtgaagacgc
     1621 tggccgtccg catgaaacag cacgacgaaa gcgggcgcgt gatggccgat tatcttagcg
     1681 gccatcgcaa ggtgaagcag gtcttttatc cggggctgaa gagccatcca cagtacgagc
     1741 tggcatgccg gcagatgtca ggttttggct ccatgatcac ctttgagacg ggttcgctgg
     1801 gcaatgccaa gcggctgctg aagcgagtgc gcgtgtgcac catgggcgaa tcgctgggcg
     1861 gggtcgaatc gctgatctcg catcccgcga gcatgacgca cgcagcgatc ggggagaagg
     1921 gaagaaaggc gatcggactg accgatggaa tggttcggat ttcggtggga attgaggata
     1981 tagaagatct gattgccgat ttagagcagg cgctgggtta gtgctcgctc ttcgctcttc
     2041 gcttttcgct attcgccttt cgctcttcgc taaaaccttc acgctgtcat cccgagcgag
     2101 gacttcagtc cgagtcgagg gacctgctct ttccttcctc ggccgcagaa tctcactgcc
     2161 ccggtccctg tgcctgtccg acgacctgat attgctcctt gtggccctac gcgttaatca
     2221 ccgtcgcttt gtttcccttc acctcaagtt gaacagtgtc gccgggctgc atcttcggct
     2281 tggtttcctt cttgcgggcg acctcatacg tggtggttgc gtcggtcagg atggagtaga
     2341 cgacccactg cccaacttta ttcttcttct gctgataggt gcagttctcc cacttcacga
     2401 ccttggcagt ctgataagtg tcagtaccca cagccacgca cgacagaaac aaggtcatca
     2461 acagtgtaaa agcaattttt ctcacaggct ctcctcggaa tttaccgtgg ttgagaaacc
     2521 gggattcgtt acaactataa gggtccttcg actacggcgg cgaagcgccg cctccaatca
     2581 ggatgacaca accggggccg tttgctaaac gccaaccgcc aaccactact caaaga
//
LOCUS       DASRKK010000450         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_524935, whole genome shotgun sequence.
ACCESSION   DASRKK010000450 DASRKK010000000
VERSION     DASRKK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524935"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            900..1703
                     /locus_tag="VFC15_10570"
     CDS             900..1703
                     /locus_tag="VFC15_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZM10642.1"
                     /translation="MKSLGNKADVAQRQRIARRQLSTDFLDHAREWRRIFAEIWGTFL
                     LVVVAAGGEVVASRSGGAVTPGMRAVAPGLMVMAIIYFMGAVSGAHLNPAVTLAFAVR
                     QNFPWSRVPGYVLGQVVGGVLAALFLRVMFGTVAALGATVPGSGIGNGKALAMEVLLT
                     TGLVNTILGTASGARNIGTNGAIAVGGYIALAGLWAAPITGASMNPARSFAPDLVRGD
                     FGTTWIYLVGPAIGALIGVIFEWILKGRPTAAGAIAAQGARDGDDSTNA"
     gene            1657..2898
                     /locus_tag="VFC15_10575"
     CDS             1657..2898
                     /locus_tag="VFC15_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM10643.1"
                     /translation="MRRKVRETAMIRRTREGGGEMNIHQATKSYENWMRSCMPVVETH
                     LRLKHKQMRKSLFLFFRGTFYRWAQLWPELCANLRDAPKVLAVGDLHVGSFGTWRDTE
                     GRLSWGVDDFDESYPLPYTNDLVRLAASVKIVIDSKKLTIGFKEGCSAILEGYQKALK
                     VGGCPFVLAEHEKTLERLGVEAFEAPDGFWEKLDHLPVVNHGLPRDAKRVLRKTLPTP
                     NLNYKVVRRQAGMGSLGQQRFVAIASWEGGHIAREAKAILPSACVWLDGHIGSRQSCY
                     EKAIQSAVRSHDPFQKIMGTWLIRRLSPESNPIEIADLPEERDEETLLYAMGSEAANV
                     HLGSKRQVANILRDLRRRKSNWLRSAARDMAKAMEGDWKKYRDSRAQFALTRQTHAHP
                     SSISLRQALSPTLLYRKTPRR"
BASE COUNT          686 a          761 c          879 g          628 t
ORIGIN      
        1 cgccaacgtt cagaggccga ggttacgtcg cgcatgagaa actgagatcc cgatagaggg
       61 aaatagggca gacagccgtt ggagaatgat gatgttcgtg agagcatcag gcaagctgaa
      121 ggacagtcgg gaccgggatg aggaatgaac gacgacatcg catcgtgaaa ccgtcatgat
      181 ggccggacgg ttaaagggca tgggccgtga ggcagaatgc gccgttaatg actctccggg
      241 agtaccacga ctggcttggt ccgaaccgtc gaggagcagg ttactacctg tagtcgattt
      301 tgcgtcggtg agagcaccca aaagcaacaa gcttcactac tgtgcaacag ggaccgtgca
      361 aatgattcaa tcaacaacag ttgcgaacta cagaattcaa taggtctcaa taattgcctg
      421 aatccggttc gggaccaggg ggtcggaggt tcaaatcctc tctccccgac cattaaaatc
      481 aacaacttgc aggcgggtag gcccgaccct ctggtgttgg cccaggtgct ctagtcctgg
      541 aaggcgcgtc agtattgggt cttccactca atccaggatg caccgccatc tacaaaagca
      601 tctacagtca gaagggattt tgctcgttga gttgtggttc cctgtggttc ttgaggactc
      661 tgccgagtgc aaattgaccg gccctgaatt tcactagaag ctaacattca gtccggaagc
      721 gatcgcattc catctggtac aggtttcgcc gggcactcca cccagccgtt tcccgttccg
      781 ggaagttcga tacgaagcga acgccctcgg cgtgatggaa ttcaattaaa cttctcttca
      841 atataggaga aggtggatgc ctaccactgg acgacccctt taggacggga accgctaaaa
      901 tgaaatcgct tggaaacaag gcagacgtag cgcagcggca gcgaatcgcc cggcggcagt
      961 tgtcgaccga cttcctggac cacgcccgtg agtggcggcg cattttcgca gaaatctggg
     1021 gcacgttcct gttggttgtg gttgctgctg gcggtgaagt tgtcgcttct agaagcggcg
     1081 gggccgtaac tccgggtatg agggcggttg cgcccggact tatggtaatg gccatcattt
     1141 atttcatggg cgcagtgagc ggagctcatt taaacccagc ggttaccttg gccttcgcgg
     1201 ttcggcagaa ctttccgtgg agccgagtgc cgggctacgt cctgggacaa gtagtcggtg
     1261 gtgtattggc ggcgcttttc ctccgcgtca tgttcggcac ggtcgctgcc cttggggcga
     1321 cagtgccagg cagtggcata ggcaacggaa aagcgttggc gatggaagtg ctcctgacaa
     1381 ctgggttggt gaacacaatc ctcggcacgg cttcaggagc acgcaacatc ggcaccaacg
     1441 gtgcaatcgc ggtgggcgga tatattgccc tggcaggact ctgggcagcg ccgatcacgg
     1501 gcgcatcgat gaacccggcg agatcgtttg cgccggactt ggtccgcggg gacttcggca
     1561 cgacgtggat ctacctggtc ggcccggcca taggcgcgct aatcggggtg attttcgagt
     1621 ggattctcaa gggcaggccc acagctgcag gcgcgattgc ggcgcaaggt gcgcgagacg
     1681 gcgatgattc gacgaacgcg tgaaggcggt ggagaaatga atattcatca ggcgacaaag
     1741 agctacgaaa actggatgcg cagttgtatg cccgtcgtcg aaactcatct gcgattgaag
     1801 cacaagcaaa tgagaaaaag cctcttcctc tttttccgcg ggacgtttta ccggtgggct
     1861 caactttggc cggagctatg cgccaacctg cgtgatgctc ccaaagtatt ggcagtggga
     1921 gatctccatg taggcagttt tggaacttgg cgtgacaccg agggtcggct gtcctggggt
     1981 gtcgatgatt tcgacgaatc gtatcctttg ccttatacaa acgatctggt gcgcctggcg
     2041 gccagcgtaa aaattgttat cgactcgaaa aagctgacaa tcggattcaa agaaggttgc
     2101 agtgccattt tggaggggta tcagaaggcg ctgaaagttg gaggttgtcc atttgttctt
     2161 gctgagcatg aaaaaaccct ggagcgacta ggagttgagg cattcgaggc ccctgatggt
     2221 ttttgggaga agctagatca tctcccggtt gtgaatcacg gcctgcctcg cgacgcgaag
     2281 agggtcttac ggaaaacgct tccaactccg aatctgaatt acaaagtggt gcgccggcaa
     2341 gctggcatgg gaagcctcgg gcaacaacga ttcgtcgcta ttgctagttg ggaaggaggc
     2401 cacatcgcgc gagaagcgaa agccatcttg ccctcggcat gcgtatggct ggatggtcac
     2461 atcggaagtc gccagtcctg ctatgagaag gctatccagt ccgcggtgcg gtcgcacgat
     2521 ccatttcaga aaatcatggg gacgtggtta attcgccgcc tctcgccgga atcaaatccc
     2581 attgagattg ccgacttgcc cgaagagcgc gacgaagaaa cgcttctcta tgccatgggc
     2641 tctgaagccg cgaatgttca cttgggaagc aaacgccaag tcgccaacat acttagggac
     2701 ctgcggcgca gaaaatcaaa ttggttgcgt tctgctgcta gggatatggc caaagccatg
     2761 gaaggcgatt ggaagaagta cagggactct agagcccagt tcgctctgac gagacaaaca
     2821 cacgctcacc cttcgtcaat ctccctgcgg caagcccttt ctcccacatt gctctatcgg
     2881 aaaaccccaa ggagataaca caccaggagg attaccaata ccgtgcctag ccctatccca
     2941 gcgccacccc cggc
//
LOCUS       DASRKK010000451         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_329417, whole genome shotgun sequence.
ACCESSION   DASRKK010000451 DASRKK010000000
VERSION     DASRKK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329417"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..668
                     /locus_tag="VFC15_10580"
     CDS             195..668
                     /locus_tag="VFC15_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10644.1"
                     /translation="MTDGLGARNDDGLSRSNHALNAALTGYFPNLPAVKGWRDFVIAM
                     CNKLPMAMRDSASPAILRRSVMGSTADRIKDIGPQPQSFDIERATKENTNYRSVAWSG
                     RYLQVTLMSIPVGGDIGLEAHPETDQFLRLEAGKGRAQMGAAKDKLTLGYPFNRS"
     gene            complement(772..1709)
                     /locus_tag="VFC15_10585"
                     /pseudo
     CDS             complement(772..1709)
                     /locus_tag="VFC15_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904945.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          489 a          726 c          706 g          444 t
ORIGIN      
        1 ggctcagcat gctcaccaac tttacgatta acaatgatcg gcttagctcg cgacggtttt
       61 ctgttcacac gtgaaagaac actcatcccc cgttctcgac tatcagaata attttttatc
      121 tgcccagggg tttgtttgcc attgccacca aggcactccg caccctgtga gcgctttaga
      181 tagagccaaa ccgcatgacg gatggcctcg gggcgagaaa cgatgacggg ttatcccgaa
      241 gcaatcacgc cctgaatgca gcattgactg ggtattttcc gaaccttccc gctgtcaaag
      301 gatggcgcga ttttgtcatc gcgatgtgca acaaattgcc gatggcaatg cgagacagtg
      361 catcgcccgc aatcttgagg agatcagtca tgggcagcac tgccgacagg atcaaagaca
      421 tcgggccaca gccgcaatcg ttcgacattg aacgcgcgac gaaggaaaac acaaactacc
      481 gttcagtcgc ctggagtggg cgctatctgc aagtgacgct gatgtcgatt cccgtgggcg
      541 gcgacatcgg ccttgaagcg catccggaga ccgaccagtt cctgcgcctc gaggctggca
      601 agggccgagc gcagatgggc gccgcgaaag ataaactgac tctggggtat ccatttaacc
      661 ggagttaggg atttgcctcc acgggcggcc cggcgaaggt cgctccaagc tgggcggatg
      721 tccgcgttgt ctcggcggct ttggcggaac agcctttggg ctctggctca taacgaggtc
      781 ttggtaacga actggtccat gcgcgacagc aagctccagg acccggtaga acagcatccc
      841 tctgctacgt gagcggcggc ggttgaagcg gaacacgaac tcgtccaggt aactgggcaa
      901 gtgtgcggaa tccaccgctc cctgatgcgt gcccagcagc catcgttttg caagggacgc
      961 tatccgatgc cctcctggca gaagttctcc ggggtcctcg cctcgcgttg aagcggcccg
     1021 ctggctacgt cggtcgtgca tgtagccatt cttctccaat ccccggtaac cctgccaggc
     1081 atcagtgatg actgtcgccc ccggttcgac gttatccaac acgaaggagt gaagcgattc
     1141 tgaggaggcg tccgccacag gcgccattcg gcagcgcccg aatcccttcg gctccaggat
     1201 ctcgatcgcg atacacgtca ggaccttctt ccctttggcg cggcctcccg aaagtccgga
     1261 ttctttgcct ccgatgtagg tttcgtccac ctccaccttc ccgcaaaggc gctcccgctc
     1321 ggggcgaatc aatacagacc gcaggcgatg aaggatcgcc cacactgtct ggtaggagcc
     1381 gatctccagg gtgcgcttca aactcagcgc cgagaccccg tctttgccca ctgcaaaatg
     1441 ccaacagacc gaaaaccaaa ccgtaagagg agtacgggtc cggtcaaaga tagtgcccgc
     1501 tatcactgat gtgcggttac cgcatcagta cacatgaacc tgccgtcccc aagccgccat
     1561 cctcccggat gaccacagga cgggcagacg aagccgcaag gccatcgtag ccattccagg
     1621 tagtccaagc agtctgcatc agtcgggaac caagcttgaa attctccaag cgacttaggg
     1681 tagtgcgtgc tggcacgggg gcgttccatc gccacagcct atgcacaact ccggttaaat
     1741 ggatacccca gagcgtgcat atgagacggg gcggttgggc ccacttcgag ggagcagagg
     1801 ggctcggaag cgtcaaacac ccccgccttc atcgaggtga ggaccttggt cggcgacgag
     1861 ataacgagac gcctgatctg ttcagtaagt ctgtctcgca gtgaccagca tctttgtcga
     1921 tgcgctccat gcggtcgctc acgcgcgggg gagggaaggc cccctttccg cgtcatgcgc
     1981 gggcttgacc cgcgcatcca gggatgggac tcgccgggct aaccgcaacc gcctccacct
     2041 gggcgcccgg atcaagtccg ggcgtgacgg aagaggaggg acggtcaggc cttggcgacg
     2101 gttgctcagg cgccctcgct gcgcgaggac gcctcaggac taggcccgcg taaagccaaa
     2161 tgcagcgtgc tctggccttc tccccgcgtg cggggagaag ggacgccgtc accgcatatt
     2221 gccggtgtgc ccgagcgagt agcggccggg ctgcggccag acggtgaggc cgtgcggcat
     2281 gccgccgacc gggatcgtct tcaccgtgcc gtcgtcggtg ttgaaggcgt agacgacgcc
     2341 gtcgtagcgg cccgacagcc acaga
//
LOCUS       DASRKK010000452        21801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_817725, whole genome shotgun sequence.
ACCESSION   DASRKK010000452 DASRKK010000000
VERSION     DASRKK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21801
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817725"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFC15_10590"
     CDS             <1..326
                     /locus_tag="VFC15_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HZM10645.1"
                     /translation="RVMFRLVGTTSNRAAIGARVTITAGGVTQLREVKGGNSYISQSD
                     LRPHFGLGKEDRIAKIEVRWPNGKTEELKDVAADAIYTIVEGSGIKDTVQLPPVAEPT
                     TTKAK"
     gene            complement(335..2056)
                     /locus_tag="VFC15_10595"
     CDS             complement(335..2056)
                     /locus_tag="VFC15_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM10646.1"
                     /translation="MASNAFSQAHAVAPPPPPGAKNVVCKDRPVPQLTDITLKTGIKF
                     QHQADPEKKYIVESMGGGVLVLDYDRDGWPDIYFTNAPNVAMALKGQTARSALYHNNH
                     DGTFTDVTDKAGVATPCFAMGGAVGDFNNDGWPDMVITCLGHTILYQNNGNGTFTDVT
                     KKAGIDDPRWSTGAAFGDYDNDGFVDLMITRYVDFQLSDLPAFGSSPTCKYRGIDVQC
                     GPRGLRGAGDSLYHNNGDGTFTEVSKQAGVDDSRGYYGLGVVWSDFNNAGRPDIYVAD
                     DSTPKYLYRNDGNGKFTDVGLESGTAVSADGSEQGSMGIAVGDYTHNGRPSLYVTNFA
                     DEYNILYLNQGDYDFRDVSYEAGVAVASLPWVKWGDAFVDLDNDGWLDLISVNGQVYP
                     QVDQLPSGARYRQPKNLFMNQGNGNFCDAATQAGPALQIPRVGRGLAVADLDNDGNVD
                     IVVEDLDGEPMILHNQGLPGKHWVSFELAGTKSNRLALGARLKLVAGGMTQAEEIRSG
                     GSYLSNHDFRVHFGLGDATKIDSLEIRWPSGKVETIKDLKSDQFYAVLEGSGVVDREK
                     ILPPKKK"
     gene            complement(2253..3656)
                     /locus_tag="VFC15_10600"
     CDS             complement(2253..3656)
                     /locus_tag="VFC15_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM10647.1"
                     /translation="MKPVTQQSASDLLGLLRTNKISALELADEYIRQIERLNPQLNAL
                     VDFDTERVRVQARASDRPGSLKGPLAGLPMTVKSSISTAGYHCEIGSLVNRGNIPTKD
                     ATVVARLRKAGATILGTTNCPEYLMAYVTDNLLHGRTNNPWNLDYTPGGSSGGESAAI
                     AAGMSAGGLGSDSGGSVRVPAHFTGICSLKPTPGRIPGDGHMPPCIGPFSFLGAIGPM
                     ARTIDDVALLFHALAGQDPSDPVSPPIALRSPSLDEIKRLPIGYFEDDGLTPVTPETR
                     QAVRDAAQALRAQGFDVRPFRPNGLEQARKLWETFFVRCGAMFEEPLVRGHEDQLSPV
                     FRDFLARAHAAGPLTADELLNAWAESDIIRGKLLSQMQEFLLLLCPVCAVPAFKHEER
                     SWTIDGQTVEYLDAMRYSQWFNLLATPAAVVPVGRSPEGLPIGVQIVGRPFEDEIVLK
                     VASVIDHEFGYVAPPIS"
     gene            complement(3653..5377)
                     /locus_tag="VFC15_10605"
     CDS             complement(3653..5377)
                     /locus_tag="VFC15_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZM10648.1"
                     /translation="MKLIRKSSLLMLLCATLATAGAAQVTFRDITRQAGINFVHNNGA
                     AGNKWLPETMGVGCAFIDYDNDGYPDILLVNGKDWTTPGKPSTLKLYHNNRNGTFTDV
                     TAKSGLGISLFGMGAAVGDYDNDGFDDIYVSALGQSHLFHNNGNGTFTDVTKTAGMLG
                     PNEFSTSAAWVDYDRDGKLDLIVVNYVQWSQQGDIYCTLDGTHKSYCTPESYKGASVR
                     LWHNLGNGKFEDATAKAGLFDNSSKSLGVAVLDVNGDGWPDLVIANDTQPNKLYINNT
                     KGGFTESAVSAGIAFSEDGVARAGMGIDAADYDHSGRPSIVIGNFANQMLALYHNEGN
                     GLFVDEAPRSEVGRRSLLTLGFATLFFDYDNDGWLDIFVANGHIENEIERIQKRVKYA
                     ESPHLFHNQGKGQFVETTQSVGPDLGVPRVGRGAAYADIDNDGDLDLLLTTNGGPAVL
                     FRNDGGNANHSLRIKLVGTKSNRDGIGTVAIVKVGGDRQTAMLRSGSGYLSSSELVLT
                     FGLGKSAQADEIELHWPSGQVDKLSKVAAGQTITVKEGSGIVQQRAFGAQTQPATPPA
                     TATRKATQ"
     gene            complement(5387..8197)
                     /locus_tag="VFC15_10610"
     CDS             complement(5387..8197)
                     /locus_tag="VFC15_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10649.1"
                     /translation="MRKLALLSISALTISAIYLYAWPSANLFYAAVVLFHVGLGVLFC
                     VGGLRLLPAAMRPPAIVRLGTLVLATGAVLGLVLIYTGASRPYTRLLLSHGAISALAV
                     ALLAGWWLSQREGKARSPWIPALGMVAVAVVISFGAKYSRDSWSKRFVIRNPQIAPLS
                     MDNEGDGAGGPFFPSSSQVAGKRKIPSKFFMESDACQRCHQDIFKEWQSSAHHFSSFN
                     NQWYRKSIEYMQDVQGTKPSKWCAGCHDPAVLYSGKMDIPIKQIVNTPEAQAGMGCMM
                     CHSIVSVKSTMGQGDFMLEYPELHELAATKNPVLRWVHDFSIKMNPEPHRRVFLKPFM
                     QKQTPEFCSSCHKVHLDVPVNNYRWFRGFNEYDNWQASGVSGMGARSFYYPPKPQGCQ
                     DCHMPDTKSNDMGNVAGKIHSHSFPAANTALPMANQDQHQMAMEQNFLKSGALTIDIF
                     AVSNAAPQTSGKGGPQTELQTTFAVGEEAEMKTTGESGGVVAPVTAPLDKASGSVRRG
                     ESARVDVVVRTRKVGHFFPGGTVDAPETWVELKAVDDKGQTLFWSGKAGDDGKGPVEP
                     GAHFYRSLQIDEHGNPINKRNAWSTRAVVYVRLVPPGAADTIHYRLNVPQNAGSKITL
                     TAKLCYRKFSWWNTHFAYAGVSTQDAVGPQTGKGYDDRQFTFDGDLTKVSGKLKDIPE
                     LPIVVLAEDTVELRVLPKGAPAEAPKTVVEKADWQRWNDYGIGLFLQGDFKGAAAAFQ
                     KVTEADPNNPDGWVNLGRVAVQEGDMERARTVLQKALALSPNLARAHYFYARVLRSDG
                     DYDGAAKELQKVIEQYPKDRVVRNDLGRVLFLSRKYPEAVVQLQQVIAIDPEDLQANY
                     NLMLCYKGMGDTAKAAEYEKLYMRFKADESAQVITGPYRRLHPEDNNERQLIHEHVSV
                     PLTPAKKTSPVAGNHVVNTAGK"
     gene            complement(8221..11679)
                     /locus_tag="VFC15_10615"
     CDS             complement(8221..11679)
                     /locus_tag="VFC15_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZM10650.1"
                     /translation="MFARLRRWMPGLVTACLLVSTGSADTPQVRAEAAKLNNLGVAMM
                     NQQLMEKAVVNFDAAYKLDPSLATAALNKGIALLNQQKLPEAEAALQLAAVKEPDNPR
                     VWYNLGLVHRGDGKTAEAITDFLRVLKIDPTDPDAYYFLGTFYSQQQDYPKAIDAFQQ
                     ALKINPLHASAEFGLARALQRSGQTDEARVHLKKFEHLTRDKISSPITLTYGEQGRYS
                     VAQDVITTDPAVGPMIPVTFVPQALAGAMKGSATASGGGVCMIDVDGDGRLDVLSIAD
                     GDPVVHVFRSLGEGKFKEEPSSQYGLALKGKGVSCAVGDYDNDGKNDLAVTLEDRVVL
                     FHNDGGGKFSDVTAKVGITPMNEPAGLTFVDYDHDGDLDLFVTGKRLNGVTDSTDNLL
                     WRNNGNNTFTNWTKEAGVGGTGATTAVVLSDINNDRAVDLIVTGSTNAPTVYLNQRES
                     LFKAMPLYDAAGLSPTVGVYIFDFNKDGWMDVALTHAGAPGISLWRNVDGKRFERVPL
                     PIADATRGWGITAVDFDNDGWIDLAVVLETSKGTELRVLRNKGAQGFEDVSAALGLNK
                     VKIASPRAVVAADLDNDLAADLLITQLGGDPVVLHNDGGNKNHALRIDLKGLADNKTG
                     LGTKIEVFANGTWQKFEVAGGAGYLSQGPPEVLAGLGKNTNADIVRMLWPTGVLQDEI
                     DVAVNKPVSFLELDRRGSSCPTLFAWNGEKYEFITDVIGAGVVGHWISPTAKNTPDPD
                     EWVKIDGSKLRAKDGYLSVRFGEPMEEVNYIDQLRMVAVDHPANIDVYPDERFLDNPP
                     FASGKAVAASTPHVPVGAWDDHGRDVLQLLSARDHLYVRDFTNLPYAGFANTHTLTLD
                     LGTWTSHNPLRLFLSGFIEYFSATSMYAAWQAGLQPMSPYIEAQMPDGSWKRVVDDMG
                     FPAGLPRTITVDLTDKLPAGVRNIRITTNLQIYWDQVLVDNGPAREHTVRTTELPLAA
                     ATLGFRGYPRQVDGETPGDLTYYYEQASTTGPFSRFRGSYTQYGDVTALLTSIDNHFA
                     VFGTGEDIDAEFDTAALPSLPSGWKRDYFFYANGFVKDMDFFEAIPFTVDEMPFHGMS
                     AYPYPASQHYPDDSATNGYRLNSNDRFESGANRATEYRFEFAPRRIDPVPLTPSRASH
                     ASAIEQ"
     gene            12082..13608
                     /locus_tag="VFC15_10620"
     CDS             12082..13608
                     /locus_tag="VFC15_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10651.1"
                     /translation="MWFPDRLRKTVWQCRSIVWIASWLVPRDRRTAWRSEQNRRFWHW
                     CHYLSESGNLTSQNRLLVAQQCWATFPDAFWIRFDRERFYARWRSLRCSPATFLASLA
                     LGLSALVLASGFVTAARTTFSSPIPHPSQVVIVTLDGTGMNGKFSRTRSDTLLDLSSI
                     WSKSKLVSGLTPFSWAPGNLLLQRRDLPVATARVGPEFFAALEVKAALGRIFAPADVL
                     DCPNCVLLSDAVWQHEFNRDANIVGKTVTLDGTPRTVIGILPRDYRMISSGIAVWSLV
                     DPAMLFTNFQRRVGAVARLRGDATAARVRLDLTDLTESAGYVHPSSQLQVTTVEAQTR
                     RNLLNTIWLVLLATGCAMFVVILRRPANNFGRLPQGLRRRTTWIAFFLAKSALLLAIS
                     ALASWCLVHWISYWLVGPVYPMVDDFSIWVFLPLAIAAMSWSVRDQQRRCRTCLRRLE
                     LPVEIGRIGSVLLNWAGIEMVCPQGHGVLYLPDSPANSLDRDRWSNLDESWSSLFRAD
                     "
     gene            complement(13673..15925)
                     /locus_tag="VFC15_10625"
     CDS             complement(13673..15925)
                     /locus_tag="VFC15_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM10652.1"
                     /translation="MTRTDPSRSARSIVTQRAEFFPSLWPVSAAAIIGFVAGWAYFAR
                     LRLANNSVVAPWNELLRAGPLLIAAVLVSAIAFSIVKLHGSELHNLQLTQSNAMLHAK
                     AESADESDERFQLMFHGNPFPTYIYDCGSLRLLDLNEAAADKYGYTLDEFLELSVPDI
                     RPDIDTSALAQELQGRGAGFNHAGVWRQCRKDGSVFFAEATVVRFVRDGREQELVLAT
                     DATQRIEAEEALRESRERLRSLVDGAPLGICWGGYMAERFETVNPAFCEMLGYSEEEA
                     LNLSLATQVYANPGERANFMEILRRNGRLKGHELTFLKKDGSPIRLRVSAVLTTHDDV
                     NLDRVEAYVEDLTEQGALEQQVRAVQKLEAVGRLAGGVAHDFNNILVVIKLSTEMMLG
                     QITPESSLSKPLLQVSNAADRAAALTKQLLAFSRRQMLQVRVVNINSIVSDTSHMLRR
                     IIGEDVQLGTHLGENLSNAKLDPDQLSQVIMNLGVNARDAMPGGGVLHIETANVELDE
                     AYAKTHPPARPGSYVMLTVSDTGTGIAKADMPRIFDPFFTTKEAGKGTGLGLAIVYGI
                     VKQSGGYIWVYSEPGQGTTFKLYFPTTNSPLEVVPWRADMAGHTTRQTILVVEDEPAI
                     RGNVRDCLHQLGYTVLEADSGEAALELCEQNQGKIELVLTDLIMPGMGGREMARQLAE
                     RFPDIEILYTTGYTEDNVARQELLQGGQSFLEKPFSVSELSQAVHRILALQTRRMEAR
                     NTKPATPGVA"
     gene            complement(16175..16675)
                     /locus_tag="VFC15_10630"
     CDS             complement(16175..16675)
                     /locus_tag="VFC15_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10653.1"
                     /translation="MTARTSAILMSLVVLIMVAPGMAFATDGGDRVQFFQSITVGPDE
                     HVGDAVCIFCSIRIAGSTGDAVAIFGNIVVDGVVNGDAVAVGGGIKLGEDASVGGDAV
                     AIGQGVYRHPNAVVKGQTVSQAGPIILLGLIIVPLLPIILVVALVVWLLRRNRYVPPA
                     QAAYRR"
     gene            16931..17887
                     /gene="msrP"
                     /locus_tag="VFC15_10635"
     CDS             16931..17887
                     /gene="msrP"
                     /locus_tag="VFC15_10635"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZM10654.1"
                     /translation="MYFKKSSDIPSSEVTPQGVYMNRRSFIAGAGALAGAAFIVPKLD
                     SVVAPSDTVIAGTKLDFSKSSYSTTEKETPLNDITHYNNYYEFSTDKYEPADLAKNFN
                     PRPWTIKVDGLVNKPRTFDIDTLLKMHLEERIYRMRCVEGWSMVIPWIGFPLSSLLSQ
                     AEPNSQAKFVQFTSLDDPKRFPGQQRAVLDWPYVEGLRMDEAMHPLTILAVGLYGDTL
                     PNQDGAPVRLVVPWKYGFKGIKAIVNIKLVEKQPVSTWTKAGPNEYGFYSNVNPNVDH
                     PRWSQAKERRIGEFLKRPTLMYNGYGDQVASMYNGMDLKKNF"
     gene            17899..18543
                     /locus_tag="VFC15_10640"
     CDS             17899..18543
                     /locus_tag="VFC15_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZM10655.1"
                     /translation="MKAKYLKPAVFLASLVPLGRLAWKAYNSALGANPIQVITWSTGT
                     WTLVFLMVTLSVTPLRKLTKQYWLIQYRRMFGLFAFFYGCLHFLTYIWLDQFFDLHSV
                     AKDVVKRKFITVGFTAFVLMIPLALTSTQRAIRWLGKRWQKLHRFIYLIATLGVVHYI
                     WLVKKDIRKPVIYAAILAVLLLYRVIVWAIEHRPVQPRMKEPATGIDRGTVAAD"
     gene            18808..20607
                     /locus_tag="VFC15_10645"
     CDS             18808..20607
                     /locus_tag="VFC15_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZM10656.1"
                     /translation="MDERSFHRLLRRAIAIPVALLVLLAIVLVIEILTLTGSLRLVDH
                     TDQVITNARQAMRYMIDMESSARGFELTGDEKFLEPFQSAKSQLPSSIDALTQLTSDN
                     SLQQARVRDIRNLDEHWIHWAELEIAAHAKKRPTEDELLQGDALMEEIRNRQREIVSE
                     EERLLHLRTRQATYLSRVVLVTAVVLSVLVAGLLFTMTRRELRELSNSYERHLNAEEE
                     KSRLLAESRGRFQITLNSLGDAVIATDANGRVRYINPVAQQLTGWDDYLQARGRPLGE
                     VARLVDERTRHELTDPVNIVRRAEKVVGLSNHVLLLSRSGQEYPIEMNGAPIMNDRGQ
                     LMGVVLVFRDVTQRRQTEQTLRASDRLTQVGRLAATIAHEIRNPLDTVSNLLFLLRHE
                     SYPNPETKNYLDLASEELARITQITGQLLTFHREAQSPVQVDLAKVLDSVLTLYAPQI
                     TMTGITVTSRFDTLCPVRGFPGELRQVFANLVVNAIHSMPDGGKIFLHIYESSLASDP
                     ERKGVRVTVLDNGAGIPPGVRKNLFAPFYTTKGEGGTGLGLWVTRGIIEKHEGTVHFI
                     STVRPGRSGTAFSVFIPFEQLLGKLDLPKPPLA"
     gene            complement(20672..>21801)
                     /locus_tag="VFC15_10650"
     CDS             complement(20672..>21801)
                     /locus_tag="VFC15_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZM10657.1"
                     /translation="KGARITSHIALPGRFLVYMPTVNHVGVSRKINSEEERQRLKRIV
                     TSERENGLGGFIVRTAAANIKEDELRADIRFLKHLWAEIKNRADTGKSPALIYHDLNL
                     VERVLRDQVNADFSQIWVDTEDEYERVVRFAQRFQPSLVRRIKLYTKAAPIYEHFGIQ
                     DEINNALKSKVWLKSGGSIVINQTEALVAIDVNTGKFVGKTQRLEDTIVKTNVDAIKE
                     IVRQIRLRDLGGIIVIDFIDMDERRNRQKVMQALEEALRSDRAPSKVLQFNDFGLVAI
                     TRKRVKQSLERTLGESCPYCVGAGFVKSVPTVCNEIYIEMRKMVHEHERTDITLRVNP
                     EVAKALKAGNGKLITEMEDLTGKTILVKSDPLLHQENFDMQ"
BASE COUNT         4331 a         6727 c         6096 g         4647 t
ORIGIN      
        1 atcgcgtgat gttcaggctg gtgggaacga cgagcaatcg cgccgccatc ggagcccgcg
       61 tgacgatcac cgcaggcggc gttacccagt tgcgcgaggt caagggcgga aatagctaca
      121 tctcgcagag cgatctgcgc ccgcatttcg ggctgggaaa agaagaccgg atcgcgaaaa
      181 tcgaggtccg ctggccgaat ggaaaaaccg aagagttgaa agacgtcgcg gccgatgcca
      241 tctacacgat cgtcgagggc tcggggatca aagataccgt gcaactcccg cccgtcgcag
      301 aaccaaccac gaccaaggcg aaatgagccg acagctactt cttcttgggc ggcaggatct
      361 tctcccgatc cacaactccg cttccttcca gcacggcata gaattggtcc gacttgagat
      421 ccttgatggt ttcgactttc cctgagggcc atcgaatttc gagcgagtcg atcttggtcg
      481 cgtcccccag cccaaagtgc acccgaaaat cgtggtttga caggtaactt ccgccgctgc
      541 ggatctcctc cgcttgcgtc atgccgccgg ccacgagttt caggcgcgct cccagcgcca
      601 gccgattgct tttcgttcca gccagttcaa agctcaccca gtgcttaccg ggtaatccct
      661 ggttatgcag gatcatgggc tcgccatcca gatcctcgac cacgatgtcc acgttgccat
      721 cgttgtcgag gtcggcgacc gccaatcccc ggcctacccg cggaatctgc agcgccgggc
      781 cagcctgcgt tgcagcatcg cagaaattac cgttcccctg gttcatgaag aggttcttgg
      841 gctgccggta gcgcgcgcca gagggcaatt ggtccacttg cggatacacc tggccattga
      901 ccgagatcag gtcgagccag ccgtcattgt cgagatcgac gaaggcgtcg ccccacttca
      961 cccacggcaa cgatgcgaca gccactcctg cctcgtagga aacatcacga aaatcgtagt
     1021 ccccctggtt gagatagagg atgttgtatt cgtcagcgaa gttggtgaca tagagcgacg
     1081 gtcgcccatt gtgtgtatag tcgccaaccg caattcccat cgagccctgt tcggagccgt
     1141 cggcgctcac cgccgttccc gattccagac cgacgtcggt gaacttgccg ttgccatcgt
     1201 tgcggtaaag atatttggga gtcgagtcgt cggccacgta tatatccgga cgtcccgcgt
     1261 tgttgaagtc agaccacacc acacccaaac cgtagtagcc gcgcgaatcg tccactccag
     1321 cctgcttgga tacttcggta aacgttccgt cgccattgtt gtgatacagc gaatctcccg
     1381 ctcctcgcag cccgcgggga ccgcattgca catcaatccc tcgatatttg cacgtgggac
     1441 tgctgccgaa ggctggcaga tcgctcagct gaaaatccac gtaacgcgtg atcatcagat
     1501 cgacaaaacc atcgttgtcg tagtcgccaa acgccgcgcc ggtggaccaa cgcgggtcgt
     1561 cgattcccgc cttcttggtg acatcggtaa acgtaccgtt gccgttgttc tggtaaagga
     1621 tggtgtgacc caggcaggtg atgaccatat cgggccatcc gtcgttgttg aagtcgccga
     1681 cagccccgcc catggcgaag catggcgtcg cgacgcccgc tttgtcagtg acgtcggtaa
     1741 aggtgccgtc gtgattgttg tggtaaagcg cgctgcgggc ggtctgtccc ttgagcgcca
     1801 tggcaacgtt gggcgcgttt gtaaagtaaa tgtcgggcca cccgtcgcga tcgtaatcga
     1861 ggaccagcac tccgccgccc atcgactcca cgatgtattt tttttcagga tcggcctggt
     1921 gctgaaactt gatgccggtc ttcagtgtga tgtcggtcaa ctggggaacg ggacggtcct
     1981 tgcaaactac gttcttcgct cccggcggag gtggcggcgc gacagcgtgg gcttgggaga
     2041 aagcgttgct tgccatgagc accgtcaata aaagagccag accggcgcgg agtcgtacga
     2101 ggattgccat aagttgatgg tccaattttc tattatcgca ggtgagtttg cgtggactct
     2161 gacgacccgc caccagcagg aagcgacctt tttctgaggt catatcttgg acgttttaca
     2221 gccgcgttgg taattcgaac ctgcgcaggt aactacgaaa ttggcggcgc aacgtacccg
     2281 aattcgtgat cgatcaccga cgctaccttc aacacaattt catcctcaaa tggacgcccc
     2341 acgatctgca cgccaatggg caatccctcg ggcgaccggc ccacgggtac gaccgccgcg
     2401 ggcgttgcca gcaaattaaa ccactgcgag taacgcatag cgtcgagata ctcgacagtt
     2461 tgcccgtcga tcgtccaact tcgctcctcg tgcttgaacg ccgggaccgc acatacagga
     2521 catagcagca ggaggaactc ctgcatctgc gaaagcagtt tcccgcggat gatatcggac
     2581 tccgcccacg cattcagcaa ctcatctgca gtcagcggac cagccgcatg cgcccttgcg
     2641 agaaaatcac gaaacactgg actcaactga tcttcgtgcc cgcgcaccag tggctcttca
     2701 aacatggctc cgcagcgcac aaagaacgtc tcccacaact tgcgcgcttg ctccaagcca
     2761 ttgggacgaa atggacgaac atcgaatccc tgcgcgcgca acgcttgcgc cgcatcccga
     2821 acagcttggc gcgtctcagg cgtgacgggc gtcagaccat cgtcttcaaa atatccgatt
     2881 ggaagccgct tgatctcgtc aaggctaggc gaacgcaacg cgatcggcgg gcttacggga
     2941 tcagaggggt cctgtcccgc cagcgcgtga aacagcagtg caacgtcgtc gatggtgcgc
     3001 gccatgggtc cgatcgcgcc aagaaacgag aacggaccaa tgcaaggtgg catgtgcccg
     3061 tcaccgggaa tgcgtcccgg cgtcggcttc agcgagcaga tgcctgtgaa gtgcgcaggc
     3121 acgcgcaccg atccgccgct gtcactgccc aagccaccgg cagacattcc ggcagcaatg
     3181 gccgccgatt ccccaccact agagccgccg ggtgtgtagt cgagattcca gggattgttg
     3241 gtgcgaccgt gcagcagatt atcggtcaca taggccatga ggtattccgg gcagttggtg
     3301 gtgccgagaa tcgtcgcgcc cgccttgcgc aagcgcgcca ccaccgtcgc atctttggtt
     3361 gggatgttcc cccggttgac caggctcccg atttcgcagt ggtaccccgc cgtcgagatc
     3421 gacgacttca cggtcatcgg cagtccggcc agggggcctt tcagactccc cgggcgatca
     3481 ctcgcacgcg cctgcacgcg aacccgctcg gtgtcgaagt ctaccagcgc attgagttgc
     3541 ggattcagtc gctcgatttg ccggatatac tcgtccgcca actcaagcgc cgagatctta
     3601 ttcgtgcgca ggaggcccag cagatcactg gccgactgct gtgtgacggg cttcattgcg
     3661 tggctttgcg agttgcagtc gctggcggcg tcgctggttg cgtctgggct ccaaacgcgc
     3721 gctgctgcac gattcccgag ccttctttca ccgtgatggt ctgtcccgcc gcaaccttcg
     3781 acaatttgtc cacctgccca ctgggccagt gaagttcgat ttcgtcggcc tgcgccgatt
     3841 tgccgagtcc gaaggtcaac accagttcgc tcgacgaaag atatcccgaa ccactacgca
     3901 gcattgcagt ctgccgatcc ccacctactt tcacgatggc gacagttccg atcccgtcgc
     3961 ggttcgactt cgtgcccacc aacttgatgc gcaagctgtg attcgcgtta ccgccgtcgt
     4021 tgcggaacaa cactgcaggg ccgccgttgg tggtcagcag gagatcgagg tcgccgtcgt
     4081 tgtcgatatc ggcatacgcg gctccgcgtc ccacgcgggg aacgccgagg tctggaccga
     4141 ccgattgagt cgtctcgaca aactggccct tgccctggtt atgaaagagg tggggagact
     4201 cggcgtactt gacgcgcttc tgaatgcgct cgatctcgtt ctcgatatgg ccattggcca
     4261 caaaaatatc gagccagcca tcgttgtcgt aatcgaagaa gagcgtcgcg aagcccagtg
     4321 tcagcaagct gcgacgcccg acttcggaac gcggcgcttc atccacgaac aagccattgc
     4381 cttcgttgtg gtagagcgcc agcatctggt tggcgaagtt gccgatcacg atgctgggtc
     4441 gtcccgagtg atcgtagtcc gccgcatcga tgcccatgcc ggctcgggcc actccatctt
     4501 cactgaaggc gatgccagcc gacaccgccg actcggtaaa gcctcccttg gtgttgttga
     4561 tgtagagctt gttaggctgg gtgtcgttgg caatcaccag gtcgggccag ccgtccccgt
     4621 tcacgtcgag caccgccact ccgagagatt tcgacgagtt gtcaaacagc ccagctttgg
     4681 ccgtggcgtc ttcaaacttc ccgttgccga ggttgtgcca caggcgcacc gacgcgccct
     4741 tgtacgactc gggagtgcaa tacgacttat gggtgccgtc aagcgtgcaa taaatgtcac
     4801 cctgctggct ccactgcacg taatttacga cgatcaggtc cagcttgccg tcgcggtcgt
     4861 aatccaccca cgccgcgctg gtgctgaact cgttcggacc cagcatcccc gcagtcttgg
     4921 taacgtcggt gaatgttccg ttaccgttgt tgtggaacag atggctctgg ccgagcgccg
     4981 agacgtaaat gtcgtcgaag ccgtcattgt cgtaatcgcc gacggccgct cccatgccga
     5041 acagcgaaat accgagcccc gacttcgcgg tcacatccgt gaacgtaccg ttgcggttgt
     5101 tgtgatagag cttaagggtt gagggcttgc ccggagtggt ccagtccttg ccattgacca
     5161 gcaggatgtc gggataccca tcgttgtcat agtcgatgaa ggcacaaccc acgcccatgg
     5221 tttccggcag ccacttgttg ccggcagctc cattgttgtg cacgaagttg atgcccgcct
     5281 gcctggtgat atcgcggaac gtgacctgcg ccgcgcccgc ggtcgcgaga gtggcacaca
     5341 gcaacatcag taaggatgac tttcgtatga gtttcatggg atccggttat tttcccgctg
     5401 tatttacgac gtgatttcca gccaccggcg aagtcttctt cgccggagta agcggaactg
     5461 aaacatgctc gtgaattaac tggcgttcgt tgttgtcctc gggatgcagc ctgcgatacg
     5521 gcccagtgat cacctgcgcc gattcgtccg ccttgaaacg catgtagagc ttctcgtact
     5581 ctgcggcctt ggcagtatcg cccatccctt tgtaacagag catcaagttg taattcgcct
     5641 gcaagtcctc gggatcgatg gcgatgacct gctgcagttg cacgaccgcc tccgggtact
     5701 tgcgcgacag aaacagcacg cgtcccagat cgtttcgcac cacgcgatct ttcgggtact
     5761 gctcgataac cttctggagt tctttggcag cgccatcgta atcgccatcg gagcgcagca
     5821 cgcgagcgta gaagtaatgc gctcgcgcca agttagggct cagcgccaga gctttctgca
     5881 gcacggttcg cgcccgctcc atatcgcctt cctgcaccgc aacgcgaccc aggttgaccc
     5941 agccgtcggg attgttggga tcggcctccg tcaccttttg gaacgccgcc gccgcgccct
     6001 tgaagtctcc ctgcaggaac aggccgatgc cgtaatcgtt ccagcgctgc cagtcggctt
     6061 tctcgacaac cgtcttcggt gcttcggccg gcgcaccttt gggaagcacg cgcaattcaa
     6121 ccgtatcttc ggcgagcacc acgatgggca actcaggaat atctttgagc ttgcccgaca
     6181 ccttggtcag atcgccatcg aacgtgaatt gccggtcgtc gtagcccttc ccggtttgtg
     6241 gaccgaccgc gtcctgcgtg gacactcctg catacgcgaa gtgcgtgttc caccacgaga
     6301 acttgcgata gcacagcttc gcagtaagcg ttatcttgct gcctgcattc tgcggcacgt
     6361 tcaaccgata gtgaatggta tcggcagctc ccggtgggac cagccgcacg taaacaactg
     6421 cccgcgtgga ccacgcattg cgcttgttga tgggattacc atgctcgtcg atctgcagcg
     6481 atcgatagaa atgcgcgccc ggctcgacgg gccccttgcc atcgtcgccc gctttgccgc
     6541 tccaaaacag cgtctgccct ttgtcgtcca ctgctttcaa ctcgacccat gtctcaggag
     6601 catcgaccgt accgccgggg aagaagtgcc caactttccg agtgcgaacc accacatcaa
     6661 cgcgcgcgct ctcgccacgg cggaccgaac cgctcgcctt gtccaacggc gccgttaccg
     6721 gagcgacgac tcctccgctc tcgccggtag tcttcatctc cgcttcttcg ccaacggcga
     6781 acgtcgtttg taactcggtc tgcgggcctc ccttcccgct ggtctgcggt gccgcattcg
     6841 acaccgcgaa gatgtcgatg gtcagtgctc cgctcttcag gaaattctgc tccatggcca
     6901 tctggtgctg gtcttgattg gccatgggca gggctgtatt agcggccgga aacgaatgcg
     6961 aatggatttt gcctgcgacg ttgcccatgt cattcgactt ggtatcgggc atgtggcaat
     7021 cctggcaacc ctgcggcttg ggcgggtaat aaaaggagcg tgcgcccatt cctgacaccc
     7081 cgctggcctg ccagttgtca tactcgttga agccgcggaa ccagcggtag ttgttcaccg
     7141 gaacgtcgag gtgcaccttg tggcacgacg aacaaaactc cggcgtctgc ttctgcatga
     7201 agggcttcag gaacacccgc cgatgcggct cgggattcat cttgatgctg aagtcatgca
     7261 cccatctcag caccgggttt ttcgtggccg caagctcatg caactcggga tactccagca
     7321 tgaaatcgcc ctgtcccatc gtgcttttca cgctgacgat ggaatgacac atcatgcaac
     7381 ccatgccagc ttgcgcttcg ggggtattca cgatctgctt tatgggaatg tccatcttgc
     7441 cgctgtacaa aacggcgggg tcgtgacagc ccgcgcacca cttcgaaggc ttggtgccct
     7501 gcacgtcctg catgtactca atgctcttgc ggtaccactg attgttgaat gacgagaagt
     7561 gatgagccga actttgccac tctttgaaga tgtcctgatg acagcgctgg caggcatccg
     7621 actccatgaa gaacttcgag ggaatcttac gcttccctgc cacctgcgat gagctcggga
     7681 agaacgggcc gccagcaccg tcaccttcgt tatccatcga gagcggtgcg atctgcggat
     7741 tccggatcac gaagcgcttg ctccaggaat cgcgcgaata cttcgcgccg aacgagatga
     7801 caactgcgac agcgaccata cccagcgccg gaatccacgg gctgcgagcc ttgccttcac
     7861 gctgactcaa ccaccaaccc gccagcaacg cgaccgcgag cgcagaaatc gcgccgtgcg
     7921 aaagcagcag cctggtatag ggtcgcgagg ccccggtata gatcaacacg agtccgagaa
     7981 ccgcgcctgt cgccagcacc agcgtcccta acctcacgat ggcgggcggg cgcatggcgg
     8041 ctggtagaag gcgcagtccc ccgacgcaga acagcacgcc taacccgacg tgaaacagga
     8101 cgacagctgc gtagaacaag ttggcagaag gccacgcata caagtagatg gccgaaatcg
     8161 tcagcgcaga aatcgaaagt aaggcaagtt ttctcatcgt ctattgctaa taacgctcgc
     8221 tcactgctca atcgcggacg catgcgacgc cctgctgggc gtcagcggca ccggatcaat
     8281 cctgcgcggc gcaaattcga atcgatattc tgtcgcgcga tttgctcctg actcgaagcg
     8341 atcattcgag ttgagccgat acccgttggt agccgaatcg tcgggatagt gctggctcgc
     8401 agggtaggga tatgcactca tcccgtgaaa cggcatttcg tcaacggtga acggaatcgc
     8461 ctcgaagaaa tccatgtctt tcacgaatcc gttggcgtag aagaagtagt cgcgcttcca
     8521 gccggaggga agcgacggca gcgcagccgt gtcaaattct gcgtcaatat cctcgcccgt
     8581 cccgaacacc gcgaagtggt tatcgatgga cgtgagcagc gcagtcacgt caccgtattg
     8641 cgtgtagctg ccgcggaagc gcgagaacgg tccggtggtg ctcgcctgtt cgtagtaata
     8701 cgtcaggtcg cccggagtct cgccgtccac ctgccgggga tagccgcgga accccagcgt
     8761 cgccgcagcc agaggcagct cggtggtccg cacggtgtgc tcgcgcgccg ggccgttatc
     8821 gaccagcacc tggtcccagt agatctgcag attggtggtg atgcgaatgt tgcggacgcc
     8881 tgctggcaat ttgtccgtca gatcgacggt gatggtgcgc ggcagcccag cgggaaatcc
     8941 catgtcgtcc actactcgtt tccacgagcc atcgggcatc tgcgcctcaa tatagggcga
     9001 catcggctgc agtcccgcct gccacgctgc gtacatggag gtcgcgctga agtactcgat
     9061 gaatccgctg aggaacagcc gcagtggatt gtgcgaagtc cacgtgccca ggtcgagcgt
     9121 cagcgtgtgc gtgttggcaa acccggcgta gggcaagttc gtgaagtcgc gaacatacag
     9181 atgatcgcgc gcgctaagca actgcaggac gtcgcgtcca tgatcgtccc atgcgcccac
     9241 cggaacatgc ggcgtagagg ccgcaaccgc cttgccgctc gcgaatggag ggttgtcgag
     9301 gaagcgctca tctggataaa catcgatatt cgccggatga tcgaccgcga ccatccgcag
     9361 ttgatcgatg tagttcactt cctccatcgg ctcgccaaaa cgcacgctga ggtaaccatc
     9421 tttggcgcgg agtttcgatc cgtcgatctt cacccactcg tcaggatcgg gagtgttctt
     9481 cgcggtcggc gaaatccagt gacccaccac gccagctcca ataacgtcgg taatgaactc
     9541 atacttctcg ccgttccacg caaacaatgt cgggcaggaa ctgccgcgcc ggtcgagttc
     9601 tagaaacgat accggcttat tcaccgcgac atcgatttcg tcctgcagca cgccagtcgg
     9661 ccatagcatg cgcacaatgt cggcattcgt gttcttgccc aacccggcga gaacttcggg
     9721 cggtccctgg ctgagatagc ccgctcctcc ggcgacttcg aacttctgcc acgttccatt
     9781 ggcgaacact tcgatcttgg ttcccaatcc cgtcttgttg tcggccaagc ctttcaggtc
     9841 gatgcgtagc gcatggtttt tgttgccacc gtcgttgtgc aacacaacag gatcgccacc
     9901 caactgcgtg atgagcaagt cggctgcgag atcgttatca aggtctgcgg caaccaccgc
     9961 gcgcggactc gctatcttga ccttgttgag cccgagcgcc gcgctgacat cttcaaatcc
    10021 ctgcgcgccc ttgttgcgca gcacgcgcag ttcagttccc ttagaggttt cgagtaccac
    10081 ggccagatcg atccatccat cgttgtcgaa atcgacggcg gtgatgcccc agccccgagt
    10141 cgcgtccgca atgggaagtg gcacacgctc gaaacgcttg ccgtcgacat tgcgccacag
    10201 gctgattccg ggagccccgg cgtgggtgag cgcaacatcc atccagccat ctttgttgaa
    10261 gtcgaaaatg tacacgccga cggtcggcga caggccagcc gcgtcgtaca acggcatcgc
    10321 tttgaacagg ctctcgcgct gattcaggta cacggtcggc gcattagtcg atccagttac
    10381 tatcagatcc accgcgcgat cgttattgat gtccgacaac acgacggccg tggtcgcccc
    10441 agtgcccccc actccggctt ctttcgtcca gttggtaaat gtgttgttgc cgttgttgcg
    10501 ccaaagcagg ttgtcagtcg agtcggtaac gccgttcaac cgcttcccgg tgacgaacaa
    10561 atcgagatcg ccatcgtggt cgtaatcgac aaaggtcagc cctgcgggtt cgttcatcgg
    10621 cgtaatgcca accttcgctg tgacatccga gaacttgccg ccgccgtcgt tatgaaacag
    10681 gaccactcga tcctcgagcg taaccgccag gtcattcttg ccgtcgttgt cgtagtcacc
    10741 gacggcgcag gagactccct tgcccttgag cgcaagtccg tactggctcg atggctcttc
    10801 cttgaacttc ccttcgccga ggctgcggaa cacatgcacc acgggatccc cgtccgcgat
    10861 cgacaacacg tccaggcggc catcgccatc tacgtcgatc atgcacacac cgcccccgga
    10921 tgcggtcgcg ctgcctttca tcgcgccggc caatgcctgc ggtacaaaag tcacgggaat
    10981 catcgggcct accgctggat cggtggtgat cacgtcctgc gcgaccgagt aacgaccctg
    11041 ctcgccgtaa gtgagcgtga tgggagagga aatcttgtcg cgcgtcaggt gctcgaactt
    11101 cttcaggtgc acgcgcgctt cgtcggtttg gccggagcgc tgcaacgcgc gcgccagccc
    11161 aaattccgcc gatgcgtgaa gtgggttgat cttcagcgct tgctgaaacg cgtcaatcgc
    11221 cttcggatag tcctgctgct ggctgtagaa cgtgccgaga aagtaatacg cgtcgggatc
    11281 tgtgggatcg attttcagaa ctcgcaggaa atcagtgatc gcctctgccg tcttgccgtc
    11341 cccgcgatgc accagcccga ggttgtacca gaccctagga ttatctggct ccttgacggc
    11401 tgccaactgc aaggcggctt ccgcctccgg aagcttctgc tggttcagca gcgcgatacc
    11461 cttgttcaaa gcggcggttg ccagcgacgg atccagcttg taggcggcat caaaattcac
    11521 gaccgctttc tccatgagtt gctggttcat catcgcgacg cccaggttat tcagcttggc
    11581 tgcttcagca cgcacctgtg gcgtatccgc cgaaccggtg cttacgagca gacaagctgt
    11641 taccaggccc ggcatccacc ggcgcagacg tgcaaacatt caggctgtcc cttcggattt
    11701 ggtgacagaa gttcacttga ttataaagct tgccaccgct tccgacttgc cttcgctgtg
    11761 ccgtcgatca tggtcccaac ccggctgttt tcgcgccagt aaaacccatg cttgatcccg
    11821 gttaccccga gaaaagtccg cccgccggtg tgtagcgcgc ttggccgctc atcctcagat
    11881 cgcaggtatt gcgattacaa cgcggccaag acgcccgaac aataatcgaa aaccactaag
    11941 aacccgcctt tggtgccatt tgcgttgatc atttgttttg cagaaccttt gtccactttt
    12001 ggggtgtcta aagacaagaa atctcggact agaatggcag gcaacgcttt ccagtgaggg
    12061 cgttgcttga ggaacggcgt gatgtggttc ccagaccgct tgcggaagac ggtgtggcaa
    12121 tgccggtcga tcgtctggat cgccagttgg ctggtgcctc gcgatcggcg tactgcctgg
    12181 cgttccgaac aaaaccgaag gttctggcac tggtgccact acctctctga gtccggaaat
    12241 ctcacctcgc agaaccgatt gctggtcgcg cagcaatgct gggcgacgtt tcccgatgca
    12301 ttctggatac gattcgatcg tgaacggttt tacgcgcgct ggcgtagcct gcgctgttct
    12361 ccagcaacct tcctggcttc gctggcgctg ggactatcgg ccctcgttct cgccagcggt
    12421 ttcgtgacgg cagctcgaac cacgttttcc tcgccgattc cccatccctc tcaggtcgtc
    12481 attgtcaccc tcgacggcac tggaatgaac gggaagttca gccggacgcg ctccgacaca
    12541 ttgctcgacc tgtcatcgat ctggagcaag tcgaagctgg tgagtgggct gacgccattt
    12601 tcctgggcac ccggcaacct gctgttgcag cggcgggact tgccggtcgc aacagcccgg
    12661 gttggacccg aattcttcgc ggcgctcgaa gtgaaggctg cgctcggtcg catcttcgcg
    12721 ccggcggatg tgctcgactg cccgaattgc gttctgctca gtgacgctgt ctggcaacac
    12781 gaatttaatc gggacgcgaa catagtcgga aagacggtga ccctcgacgg cacgcccagg
    12841 accgtgatcg gaatcctgcc tcgtgattac cggatgattt cgtcaggcat cgccgtttgg
    12901 agcctggtcg atcccgcaat gttgtttacc aatttccagc gccgggttgg ggccgttgcc
    12961 cgcttgcgag gcgacgcgac cgccgcgcgc gtccgcctcg acttgaccga cctcaccgag
    13021 agtgcgggat atgtccatcc ctcctcgcag ttgcaggtca ccacggttga ggcacaaacg
    13081 cggcgcaacc ttctgaacac gatctggctg gtgctgctgg caacgggctg cgccatgttc
    13141 gtggtcatcc tccgtcgccc cgccaacaac ttcggacgcc tcccacaagg gctccgcaga
    13201 cggacaacgt ggattgcgtt cttcctggcg aagtccgctt tgctgctggc aatttcagct
    13261 ctggcatcct ggtgtctggt gcattggatc tcttactggc tggtggggcc ggtttatccg
    13321 atggtggacg atttctccat ctgggttttt ctgccgctgg caatcgccgc catgtcgtgg
    13381 tcggtccggg accagcagag gcgctgccgc acctgtttgc ggcgcctgga attgcccgtc
    13441 gagattgggc gcattggatc cgtgttactc aattgggcgg gaatcgaaat ggtctgcccc
    13501 cagggacacg gcgtgttgta tctgcccgat tctccggcca attcgctcga tcgcgatcgc
    13561 tggagcaatc tggatgaatc ctggtcgagc ctgtttcgcg ccgattgatt cttacgagag
    13621 cacgggaccc aacgaccgat gtcgagtacc ccgtattgca ccggggggga cttcaagcga
    13681 cgcctggggt tgcgggtttc gtattacgcg cttccatccg gcgggtctgc agcgccagta
    13741 tccgatggac cgcctgggac agttcggaaa cactgaatgg tttctccagg aacgattgtc
    13801 ccccttgcaa caattcctgc cgcgcgacgt tgtcttccgt gtatcccgtt gtatagagaa
    13861 tttcaatgtc tgggaaacgt tccgccagtt gccttgccat ctcccgtcca cccatgccag
    13921 gcatgatgag gtcagtaagg actagttcga tctttccctg gttctgctcg cacagttcca
    13981 gcgcggcttc accactgtcc gcttccagca cggtgtaacc cagttgatgc aggcagtccc
    14041 ggacgttgcc tcgaattgca ggttcatctt ccaccaccag aatggtctgt ctggttgtgt
    14101 ggcccgccat gtcagcacgc cacggcacca cttctagagg gctgttggtc gtggggaaat
    14161 acaatttgaa cgtggtgccc tgccccggct cgctgtaaac ccagatgtat cccccgctct
    14221 gctttactat tccatacaca atcgccaatc ccagtcccgt tcccttgccg gcttccttgg
    14281 tcgtaaagaa gggatcgaag atccgcggca tgtcggcctt ggcaatgccc gttccggtat
    14341 ccgagacggt cagcatgacg tagcttcccg gccgcgccgg aggatgtgtc ttggcgtacg
    14401 cttcatccag ctcaacgttt gcggtctcga tgtgcaggac tccgcctccg ggcatcgcgt
    14461 cccgcgcgtt caccccgagg ttcatgatca cctggctaag ctgatcggga tccagcttcg
    14521 cattcgacag gttttcgccg agatgggttc cgagttgaac gtcttctcca atgatgcggc
    14581 gtaacatgtg cgacgtgtcg ctcacgatcg aattgatgtt caccactcgc acctgtaaca
    14641 tctgccgccg gctgaacgcc agcaattgct tggtgagtgc ggccgcccgg tcggccgcat
    14701 tggaaacctg caacagaggt ttgctcaacg aactttccgg cgtgatctgg cccagcatca
    14761 tctcggtcga cagcttgatg accaccagaa tgttattgaa gtcgtgcgcg acgccgcccg
    14821 cgaggcggcc aacagcttcc agcttctgta ccgcccgcac ttgctgctcg agcgcgccct
    14881 gctcggtcaa gtcttcaacg taggcttcca cccggtccaa attcacgtcg tcatgcgtgg
    14941 tgaggaccgc cgacacgcgc agccgaatcg gacttccatc cttcttcaaa aacgtcagct
    15001 cgtggccttt cagtctcccg ttgcgccgca ggatctccat gaaatttgcc cgctcgcctg
    15061 ggttggcgta cacctgcgtc gcgagtgaca gattgagcgc ctcttcctcg gagtagccca
    15121 acatctcgca gaacgccgga tttaccgtct cgaatcgttc tgccatgtac ccgccccagc
    15181 agatgccaag aggtgccccg tccaccaggg aacgcagtct ttccctggat tcccgcagcg
    15241 cttcctccgc ttcaatcctc tgcgttgcgt cggttgccag caccagttcc tgctcgcgcc
    15301 cgtctctcac aaacctgacg accgtagcct cggcaaaaaa taccgatccg tcttttcgac
    15361 attgacgcca gactccggca tgattaaatc ccgcgccccg gccctgcagc tcttgcgcca
    15421 gggcggaggt gtcgatgtcc ggtctgatgt cgggaacgct caattccaga aactcgtcga
    15481 gcgtgtatcc gtatttgtcg gcggccgctt cattgaggtc cagcaagcgc aggctcccgc
    15541 agtcgtagat ataggtagga aaaggattgc catggaacat caactggaag cgctcgtcgg
    15601 attcatcggc gctttcggct ttcgcatgca gcatcgcgtt ggattgtgtc agttggaggt
    15661 tgtgaagttc gcttccgtgc agcttaacaa tggagaaagc gatggcactc acaagaacgg
    15721 cggcgatcag cagcggaccg gctcgcagta actcattcca gggcgcaacc acagagttgt
    15781 ttgccaggcg cagtctggcg aagtacgccc agccggcaac aaatccgatg atcgctgccg
    15841 cgctcaccgg ccacaggctg gggaaaaatt ctgctcgttg cgttacgatc gatcttgcgg
    15901 aacgactcgg gtcggttcgc gtcatccaac gcacccccct aggtccaacg tcgggacctg
    15961 tcatccatcc gcaagcgacg aagctggcgc ggactgggac ttaatggcgg ttagacacta
    16021 gcactaatag ggaggtactt gctgtgatgc agctcacatc tcgacgtaac taattgcaaa
    16081 gtcaagcaac tttgctattc cgaccacccg caagactccc cgatagccga actctataaa
    16141 ctccagactc cggatacccg ccgcgctacc ccgcttagcg gcggtaggca gcttgtgcgg
    16201 gagggacata ccggtttcgt cgcagcaacc agaccaccag cgccaccacg agaattatgg
    16261 gcaacaacgg aacaatgatt agaccgagaa ggatgatggg accggcttgg ctgacggttt
    16321 ggcctttcac caccgcgttg gggtgccggt aaaccccttg tccaatggcc accgcatcgc
    16381 cgcccacgct ggcgtcttcc ccgagcttaa ttcccccgcc cacggccacc gcatctccat
    16441 tcaccacacc gtccaccacg atattgccga agatcgccac cgcgtcaccg gtagaaccgg
    16501 ctattcggat ggagcagaaa atgcagaccg cgtcaccaac atgttcgtcg ggacctactg
    16561 taatgctctg gaagaattgc acgcggtcgc ctccatccgt ggcaaacgcc atgccggggg
    16621 caaccatgat cagcactaca agggacatca agatcgcaga cgtgcgggcg gtcataagtt
    16681 ccatctcctg ccggatacac tactggattg atgaaatata cggaagtcgg tgggatttag
    16741 ttcccgggat tgctatgtgg attccaaggc gtcgcctggc tcttttgtgc catacgctgt
    16801 cctaccagcc tgaatacgtc acccacccca ggaaaaccag aatcatccac gttgtatcta
    16861 aatccaagct ggcgaatcaa tacgaatacc agatagccac caaggttagt ggatcgacca
    16921 ggagtcgtgg atgtatttca agaagtcgtc cgacataccg tcatcggaag tcacccccca
    16981 gggcgtttat atgaatcgac gcagtttcat cgccggagca ggggctttgg ctggggctgc
    17041 gttcattgtt cccaagctgg acagcgtagt tgcgccgtcc gacacggtta ttgcgggcac
    17101 gaagctggac ttcagtaaga gctcctacag cacaaccgag aaggaaaccc cgctcaacga
    17161 cattacgcac tacaacaatt attacgagtt cagcaccgac aaatacgaac ctgcagatct
    17221 ggccaagaac ttcaatccgc gtccctggac gatcaaggtc gatggcctgg tcaacaaacc
    17281 caggacattc gacatcgaca cgctgctcaa gatgcatctg gaagagcgca tctatcgcat
    17341 gcgctgcgtc gaaggctggt cgatggtgat tccctggatc ggatttcccc tgagttcttt
    17401 gctgagccag gcggagccga actcccaggc caagttcgta caattcacct cgctcgacga
    17461 tccgaagcgg tttcccggtc agcagcgcgc cgtgctcgac tggccttatg tcgaaggttt
    17521 gcggatggac gaagccatgc acccgctcac gatacttgcc gtcgggcttt acggcgacac
    17581 ccttcccaac caggacggcg caccggtccg actggttgtc ccctggaagt atggcttcaa
    17641 gggcatcaag gcgatcgtga acatcaagct ggttgagaag cagccggttt cgacgtggac
    17701 gaaggccggc cccaacgagt acgggttcta ctccaacgtc aatcccaatg tcgatcatcc
    17761 ccgctggagc caggccaagg agcgccgcat cggggagttc ctgaaacggc caaccctgat
    17821 gtacaacggc tacggtgacc aggtcgcatc catgtacaac gggatggacc tgaagaagaa
    17881 cttctgaaag ccggcccagt gaaagcgaaa tatctcaagc ccgcggtttt cttggccagt
    17941 ctcgttcccc tgggacggct ggcctggaag gcctacaact ccgcattggg cgccaacccc
    18001 attcaagtca ttacctggtc gacgggcacg tggacgctgg tgttcctgat ggtgacactg
    18061 tccgtcacgc cgcttcgcaa gctgacgaag cagtattggc tcatccaata ccggcggatg
    18121 tttggtttgt tcgcgttttt ctacggctgc ctgcactttc tcacctacat ctggctcgac
    18181 cagtttttcg acttgcacag cgtcgccaaa gacgtggtga aacgcaagtt tattaccgtc
    18241 ggattcaccg ccttcgtcct gatgatcccc ctcgcgctga cttccaccca gcgtgccatc
    18301 cgctggctgg gcaagcgctg gcagaagctt caccgtttca tttacctgat tgcgacgctc
    18361 ggcgttgtgc attacatctg gctggtgaag aaagacattc gcaagcccgt tatctacgcc
    18421 gctattctcg ccgttctctt gctctatcgc gtgattgtct gggcaataga acatagaccg
    18481 gtccagcccc gcatgaaaga gccagccacc ggcatcgatc gcggtaccgt agccgctgac
    18541 tagagcggat taggattttg tgtagccgga tttgtcggct atcgggagtt gatagctcaa
    18601 cgggattcat caccaccacg gagacaccga gttcacggag acgctgaaag aaataaaaac
    18661 tccgtgtcct ccgtgcctcc gtggtgaagt atctcgaacg gatacagaaa tttctaattt
    18721 ggtctagcgt cacttcgacg cgagggcaaa tcgaattgcg atatcatcgt gatggtccac
    18781 aagccagctc tgtagaccgc ctccaccatg gatgaacgca gtttccaccg actgctgcga
    18841 cgcgcgatag ccattcccgt ggctctactt gtgctgcttg cgatcgtact ggtaatcgag
    18901 atcctcaccc tgaccgggtc gctgcgcctg gttgaccaca ccgaccaggt catcaccaac
    18961 gcccgccagg ccatgcgcta catgattgac atggagtcca gcgcccgggg cttcgagttg
    19021 accggcgatg agaagtttct ggaacccttt cagagcgcca agtcgcaact tccctccagc
    19081 atcgatgccc tgacccaatt gaccagcgat aactccctgc agcaggcgcg cgttcgcgac
    19141 attcgcaacc tcgatgaaca ctggattcac tgggcggaac ttgaaatcgc tgcccacgcc
    19201 aagaagcggc ccacggaaga cgagctgctg caaggcgacg ccctcatgga agaaatccgc
    19261 aaccgccaga gggagatcgt ctctgaagaa gaacgactcc tccatttgcg gacccgccaa
    19321 gccacctact tgagtcgggt ggtgctggtt accgcggtcg tgctgtctgt gctggttgca
    19381 ggtctgttat tcaccatgac gcgccgcgaa ttgcgagaac tttccaactc ctacgaaagg
    19441 cacctgaacg ccgaggagga aaagagccgg ctgttggcgg aaagccgcgg acgtttccag
    19501 atcacgctca acagcttggg cgatgcggtc attgccacgg atgcgaacgg cagagtgcga
    19561 tatatcaatc ccgtggcgca gcagctcacc ggatgggacg attacttgca ggcccgaggc
    19621 cgtcctttgg gtgaggtggc gcgtctcgtc gacgagcgca ctcgccatga actgaccgac
    19681 cctgtcaaca tcgtgcgccg agcggaaaag gtggtcggcc tttccaatca cgtgctgctc
    19741 ctcagccgct ctgggcaaga gtatcccatc gagatgaacg gcgcccccat catgaacgat
    19801 cgcggccaac tgatgggagt ggtgctggtt ttccgcgacg ttacccagcg ccgccagacc
    19861 gagcagacgc tccgcgccag cgatcgcctc acccaggtgg gaagactggc ggcaacgatc
    19921 gcgcacgaaa ttcgcaaccc actggacacc gtctccaacc tgctgtttct attgcggcat
    19981 gaatcctacc cgaaccccga aaccaagaac tatctcgacc tggctagcga ggaactggcg
    20041 cgcatcaccc agatcacagg acaactgctg acctttcacc gtgaggccca gtctcccgtg
    20101 caggtggacc tcgccaaagt cctggacagc gtgctcactt tgtatgcccc gcagatcacg
    20161 atgactggca ttaccgtgac atcccggttc gacacgctct gcccagtgcg tggctttccc
    20221 ggcgagttgc gccaggtttt cgccaacctc gtcgtcaatg cgatccacag catgccggac
    20281 ggcgggaaga ttttcctgca catttatgaa tcgagcctgg cgtcagaccc ggagcgcaag
    20341 ggagtcaggg tgaccgtgct cgacaacggt gccggtattc ctccaggcgt gcgcaaaaac
    20401 ttgttcgcgc cgttctacac caccaagggc gaaggtggca ccggacttgg tttgtgggtg
    20461 actcgcggaa tcatcgagaa gcacgaaggc accgttcact tcatcagcac cgtgcggccc
    20521 ggcagaagtg ggaccgcgtt ttcggtcttc atcccctttg agcaattgct tggcaaactg
    20581 gacttgccca agccgcctct cgcctaaccg tcaacagcgc agtaaaaagc cgggcgcgat
    20641 ggcccggcaa ttcattgcgc gtaagtccgc gctactgcat atcgaagttt tcctggtgca
    20701 gcagaggatc gctcttcacc aggatcgtct taccggtcag gtcttccatc tcggtaatga
    20761 gcttgccatt gccggccttc agggctttcg cgacttcggg attcacccgc agagtgatgt
    20821 cggtgcgctc gtgctcgtgc accatcttgc gcatctcgat gtaaatctcg ttgcacaccg
    20881 tcgggaccga tttcacaaag ccggctccga cgcaataggg gcaggactcg cccagcgtcc
    20941 gctccagcga ctgcttgacg cgcttacggg tgatcgccac caatccaaaa tcgttgaact
    21001 ggagcacctt cgacggcgca cggtcggagc gcagcgcctc ttccagagcc tgcatcacct
    21061 tttgccggtt ccggcgttcg tccatgtcga tgaagtcgat cacgatgatg ccgcccaggt
    21121 cgcgcagccg gatctgccgc acgatctcct tgatggcatc cacgttcgtc ttcacgatgg
    21181 tgtcttccag ccgctgtgtc ttaccgacaa atttgccggt attgacgtca atcgccacca
    21241 gcgcttcggt ctggttgatc acgatcgacc cgccgctctt cagccacacc ttcgacttca
    21301 gcgcattgtt gatttcgtcc tggatgccaa agtgctcata gatcggggcg gctttcgtgt
    21361 acagcttgat gcgcctcacc agcgatggct gaaagcgttg cgcgaagcgc accacgcgct
    21421 cgtactcgtc ttccgtgtcc acccagattt gcgagaagtc ggcgttcacc tggtcgcgca
    21481 gcacgcgctc caccaggttg aggtcgtgat agatcaacgc gggagacttg ccggtatcgg
    21541 cgcggttctt gatctccgcc cacaggtgtt tcaggaagcg gatgtcggcg cgcagctcgt
    21601 cttccttgat gttggctgca gccgtgcgca caatgaagcc gcccagtccg ttctcgcgtt
    21661 cgctggtgac gatgcgctta aggcgctgac gctcttcttc ggaattgatt ttgcgcgaca
    21721 cgcccacgtg gttcaccgtc ggcatgtaca ccaggaaccg ccctggcagc gcaatgtgac
    21781 tggtgatgcg agcgcctttc t
//
LOCUS       DASRKK010000453         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_817828, whole genome shotgun sequence.
ACCESSION   DASRKK010000453 DASRKK010000000
VERSION     DASRKK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817828"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..762)
                     /locus_tag="VFC15_10655"
     CDS             complement(1..762)
                     /locus_tag="VFC15_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10658.1"
                     /translation="MKNLIAVSMVYGGLLVTLLGTVSAVWPLRLLAIRSRVTGAIVLF
                     CGVLIFVIGAILPAGETRIPSAQTKLDEFAPVYQFSESHSIQIHSSRERVYEAIRQVT
                     AGEIKLLRTLTWIRRLGRETPPGVLNAPQNEPVLDVALRSGFLLLADEPGHEIVIGAL
                     VRAPAGWRPSKKPTPDGFKSLSAPGFALASMNFLIEEAGPNRCVVTTETRVFTTDPSS
                     RRKFGEYWRVIYPGSALIRRMWLQAIKKRAESSLG"
     gene            complement(779..1261)
                     /locus_tag="VFC15_10660"
     CDS             complement(779..1261)
                     /locus_tag="VFC15_10660"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZM10659.1"
                     /translation="MPEKETLERAREDAREGKAPSTQAGEFVKEEMDHIREGKHGAAS
                     TKQAIAIGLSKARRAGVKLPPPGKGKTSEATRKKAEGDLRKGQSSAKTKTSAKRSRAI
                     KRVLKREGHQAASHAALSKQARQSARTRGSAARKASAKKASQTKGPAGRKKAARRIVG
                     "
     gene            1312..>2931
                     /locus_tag="VFC15_10665"
     CDS             1312..>2931
                     /locus_tag="VFC15_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM10660.1"
                     /translation="MDYTNPARSRADMPAAVAQSVLATPAMRSHKYRSALFAAALLLA
                     LTAIVYSAAWMYYVRLKPTVEIGIDTNYLAQAIEITNVYRDSPAEKAGLKPHDRIVAI
                     NGSSADSASAWDALLFHVWLSSHPGDTVALTVQRPGQSQPLVIRPWFRALQGAGDTKT
                     LARTVADQILESYPLLFLIVGLAVLFLRVEDRNAWLLALVFATFITAADMPNEFAAAT
                     PNFRFLLLAYRTLMGSVLTGLFYFFFAVFPTRSPIDRKVPWLKWALPVIGACLCLGGY
                     RHGNSRALPFILAVVPDPVAQTARQITSYGALLLGVLSLLLNVLSPSNVAERRKLKVI
                     LWGTAVGITPIVIVKAAEDFVGFRTPFWLNFVDVLLLFLFPLSFAYAVVKHRVMEIPV
                     LLKRSARYFVVERGFVFLILAISVGATFWLAQAFSRHFSAGSKAAIPVGATFGVLLIS
                     GATQVHRRVRTRLDRAFFRSSYDAQQILENLAAKTLTVSSREGLAALLHDQIQDALHP
                     ISMYVYLEAGNGQLQAYAGNPPAEAMTLSTSG"
BASE COUNT          518 a          923 c          832 g          658 t
ORIGIN      
        1 tcatcccagg ctgctttcgg cgcgtttctt gatcgcctgc aaccacattc gccggatcaa
       61 ggcgcttccg ggatagatca cccgccagta ctccccaaac ttgcggcgcg acgatgggtc
      121 ggtagtaaac acacgtgtct ccgttgtaac cacgcatcgg ttcggtcccg cttcttcgat
      181 caggaagttc atagacgcca gcgcaaaacc gggcgcgctg agcgatttga agccgtcggg
      241 ggttggcttc ttgcttggcc gccagcctgc gggggcgcgc accagcgcac cgataacgat
      301 ctcgtggccc ggctcatcag caagcaaaag aaagcccgac cgcaaagcga catccagaac
      361 tggctcattt tgcggcgcgt tcaaaacgcc gggtggggtt tcgcgcccga gacggcgaat
      421 ccaggtgagc gtccgcaaaa gcttgatctc tccggcagtc acctgacgga ttgcctcgta
      481 gacgcgctcc ctggacgagt ggatctgaat cgaatgggat tcgctgaact gatagaccgg
      541 tgcgaactca tccagcttcg tctgtgcaga cgggatccgc gtttcgcccg cgggaagaat
      601 ggcgccgatt acgaaaatta gaactccaca gaagagaaca atggcgccgg tgacacgact
      661 tcgaattgcc agcagccgca aaggccagac cgcagagact gttcccagca acgtcaccag
      721 cagcccgccg taaaccatcg aaacagcaat taggtttttc atggactgag atttccggtc
      781 agccaacaat tctgcgcgcc gccttcttgc gccctgccgg gcctttggtc tgactggcct
      841 tcttcgccga cgccttccgc gcagcgcttc cccgtgtccg ggccgactgc cgggcctgct
      901 tcgacagcgc cgcgtgcgac gccgcctgat ggccttcgcg tttcaacacg cgtttgatcg
      961 cccgcgaacg tttggcggat gttttcgtct ttgccgacga ctggccttta cgcaaatcgc
     1021 cttcggcctt ttttcgcgtc gcctccgagg tcttcccctt ccctggcggg ggcagtttca
     1081 ctccggcacg gcgcgccttc gacaagccaa tagctatggc ctgtttggtc gaggccgcgc
     1141 cgtgcttgcc ttcgcggatg tggtccatct cctctttcac aaattcgccg gcctgggtgc
     1201 tcggcgcttt tccctcgcgt gcatcttcgc gggcgcgctc caatgtttct ttctctggca
     1261 tcgaattccc tcctcgtacc ttcaacagag atgcagaatc cgggctgcgg gatggactac
     1321 actaaccctg cgcgcagccg cgctgacatg cctgcagccg tagctcaatc cgtcctggca
     1381 acccccgcca tgcgatcgca caaataccgg tcggcgcttt ttgcggcggc gctcttgctt
     1441 gcgctgaccg ccatcgtgta cagcgcggcc tggatgtatt acgttcgcct caaaccaacg
     1501 gtggagatcg gcattgatac caattacctc gcgcaggcga ttgagatcac caatgtttat
     1561 cgggacagcc cggcggagaa ggccggcctg aagccgcacg accgcattgt tgcgattaat
     1621 ggaagcagcg cagattcggc atcggcgtgg gacgcattgt tgtttcacgt gtggctaagc
     1681 tcacatcccg gagacacagt ggccctgacc gtgcagcggc ccggacaatc gcagcctttg
     1741 gttatcaggc cttggtttcg cgccctacaa ggcgctggcg ataccaagac tcttgcccgc
     1801 accgtagcgg accagatcct ggagtcgtac ccgctgctat tcctcatcgt gggattggct
     1861 gtcctgtttc tgcgggtaga ggaccgcaat gcgtggctgc tggcgctggt attcgcgacc
     1921 ttcatcacgg cggcggacat gccaaacgaa tttgccgctg cgacgccaaa tttccggttc
     1981 ttgctactcg cctaccggac cctcatgggc agcgtcctca ccggattgtt ctacttcttc
     2041 tttgcggttt tccccacgcg gtctccgatt gaccgcaaag ttccctggct gaagtgggca
     2101 ctgccggtaa tcggcgcctg cctatgcctt ggaggttacc ggcacggcaa ctccagagcc
     2161 ttacccttta tcctcgcggt agtgcccgat cccgtcgccc agactgcgag acagatcacc
     2221 tcttacggag cccttttgct tggcgtgttg tccctactct taaacgtact gagcccttcc
     2281 aacgtcgccg agcgccgcaa gctcaaagtc attctctggg gaaccgccgt cgggattaca
     2341 cctattgtaa ttgtgaaggc ggcggaggac ttcgtcgggt tccgcactcc cttctggttg
     2401 aacttcgtcg atgtcttgct gctcttctta ttccccttgt cattcgctta tgcagtggtg
     2461 aaacaccggg tgatggagat tcccgttctg ctgaagcgca gcgcccgcta ttttgtggtg
     2521 gaacgcggat tcgttttcct catcctggcg atttcagtgg gggcgacctt ctggttggcg
     2581 caggcgttct cccgccattt ctccgctgga tcaaaggcgg ccattccagt gggcgcgacc
     2641 ttcggggtgc tgctgatctc gggcgcgacc caagtgcatc ggcgggtgcg aacgcggttg
     2701 gaccgcgcct tcttccgtag ctcttatgac gcacaacaga ttctggagaa cctggccgcg
     2761 aagacactga ctgtcagcag ccgcgaagga ctggcggcgc tgttgcatga ccagattcaa
     2821 gatgcgctgc atccgatctc gatgtatgtg tacctcgagg ccggcaacgg acaattgcag
     2881 gcgtacgccg ggaatcctcc tgccgaggcg atgacgctct cgaccagcgg g
//
LOCUS       DASRKK010000454         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1209309, whole genome shotgun sequence.
ACCESSION   DASRKK010000454 DASRKK010000000
VERSION     DASRKK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209309"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFC15_10670"
     CDS             complement(<1..774)
                     /locus_tag="VFC15_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10661.1"
                     /translation="MKRREFIKTTAALTGASALARSINKEPANADRSIQRLFVSEVED
                     KPWFYDREMWPHYLAMLAENRFTRFNLSFGIGYDFLREVTDAYFLFAYPFFLSVPGYQ
                     VRAVNLPDAERDRNLETLRFVSDQAASHGLQFQLGIWTHGYQWEKSPNANYTIQGLNG
                     ENHGSYCRDALTALLRACPAITGITLRVHGESGVAEGSYAFWGAVFDGVKRCGRKAEI
                     DLHAKGIDQKTIDLALATGMPVKVSPKFCAEHMGMPYHQA"
     gene            complement(819..2426)
                     /locus_tag="VFC15_10675"
     CDS             complement(819..2426)
                     /locus_tag="VFC15_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10662.1"
                     /translation="MSIPRTVAEVIREHVCLEVEGIDRMYLNVYVPTLQREGGVASFF
                     RFHRGYQFASSALMDPISKAFVAEMEQFAKQGNIPVVRFGKGQRKDDVAAEYRKKFQG
                     QEGVLFIGKAQEKTPVFRTERRRNEKTGATYPWLVRSTAMVNHFYVYCMDRDFGPFFL
                     KFCTYFPYTAKLCLNGHEYAKQQLNNRGIGYEALDNGILSCEDPRRVQTICDGLSAEK
                     IDGLLRKWFRKLPHPFTAKDRQAGYRYRISILQAEFSLTQVLDRPVTGRVFFEEVIRE
                     NLDIGRPSQVQLIFDRRVNRRTPGRFRTRVITEGVVPSLHVDYKNSRIKQYHKEGRAL
                     RTETTINNTRDFGIGKLLTNLPALRQVGFQANRRLLDVQTVSHDCSIGEDAFEKVVRP
                     ITVDGQRASALRFGDSQVQALFAVLVLFSLQLRGFTNREMRALLAQLLGLNPAQYPIG
                     RMTYDLRRLRLHGLIERIPHSHRYQTTADGLKIALFFSRTYARLLRPKLAEVLCQSPP
                     SDSSLRAAFDRLQTEIDRCCQEQKLAA"
BASE COUNT          609 a          765 c          798 g          607 t
ORIGIN      
        1 ggcctgatgg tacggcatcc ccatgtgctc agcacagaat tttggcgata ccttgacggg
       61 catccccgta gctaacgcga gatcgatcgt tttctgatcg atgccctttg cgtgcagatc
      121 gatctccgcc ttgcggccgc aacgcttcac gccatcgaac acggcgcccc agaaagcgta
      181 gctgccttcg gccacgccgc tttcgccgtg gacgcgcaga gtaatcccgg ttatcgctgg
      241 acaggcgcgc agaagtgctg tgagggcatc gcggcagtac gagccatgat tttcaccatt
      301 gagtccctgg atagtgtaat tcgcattcgg acttttctcc cattggtagc catgggtcca
      361 aattcctaac tggaactgaa gaccatgact ggcagcttgg tcgctcacga agcgcagtgt
      421 ctcgagattg cggtcgcgtt cggcatccgg caggttgacc gcgcgtacct ggtagcctgg
      481 caccgaaaga aagaagggat aggcgaacag aaagtaagca tcggtgacct cgcgcaggaa
      541 atcgtaccca atgccgaagc tcagattgaa gcgggtgaag cgattctccg cgagcatcgc
      601 caggtagtgc ggccacatct cgcggtcgta gaaccagggt ttgtcttcga cctcgctgac
      661 gaacaggcgt tggatgctgc ggtctgcgtt agcgggctct ttattaatag atcgcgccag
      721 ggcagaagcg ccggttagtg cggcagtcgt cttgataaat tcgcggcgtt tcactagagt
      781 ccttgcccaa gagattctaa ggtgaatgag tcaagttttc aggcggcgag cttttgctcc
      841 tgacaacagc gatctatttc ggtttgaagg cgatcaaaag cggcgcgcag agaggaatcc
      901 gatggtggac tttgacaaag aacctcggcg agtttggggc gtaacaaacg ggcataggtt
      961 cgggagaaga agagcgcgat tttcagtccg tccgccgtgg tctgataacg atgactgtgt
     1021 gggatgcgct cgatgagccc gtgcaggcga agccggcgga ggtcgtaggt cattctgcca
     1081 atggggtatt gggctgggtt gaggcccagc agttgagcca gtaaggctct catctcgcga
     1141 ttcgtgaaac cccgcagttg gagactgaac agtaccaaca cggcgaacag agcctgaacc
     1201 tgtgagtcgc caaaacgtaa tgcggacgcc ctttgtccgt cgacggtgat gggacgaacc
     1261 accttctcaa acgcgtcttc gccgatcgag caatcgtggg aaacagtttg gacgtccaga
     1321 agacgccgat tggcctgaaa gccgacctgc cgcagagcgg gcagatttgt gagcagtttg
     1381 ccgatgccga agtcccgcgt gttgttgata gtggtttctg tgcgcaacgc gcgcccttcc
     1441 ttgtgatact gcttgatccg ggagttcttg tagtcgacgt gcagggaagg gactacgcct
     1501 tctgtgatca cccgcgtgcg aaaccgaccg ggagtgcggc ggttaacccg acgatcaaag
     1561 atcaactgta cctggctcgg ccgcccgata tccagatttt cccgaatcac ttcctcgaaa
     1621 aagacccgtc cggttaccgg ccggtccagc acctgggtga gagaaaactc cgcctggagg
     1681 atggaaatcc ggtaccggta gccggcctgc cggtctttgg cggtgaaggg atggggcagt
     1741 ttgcggaacc atttgcgcag cagtccatcg atcttctccg ccgacaagcc gtcacaaatc
     1801 gtctgcaccc gccgcggatc ttcgcacgac aggatgccgt tatccagagc ctcataaccg
     1861 atgccccgat tgttcagttg ttgtttcgca tactcatggc cgttcaggca aagcttggct
     1921 gtgtagggaa agtatgtgca gaatttgagg aagaatggac cgaagtcccg atccatgcag
     1981 tacacgtaga agtgattgac catcgcggtg gatcgcacca gccagggata cgtggccccc
     2041 gttttttcat tacgccgccg ctccgtgcgg aacaccggtg tcttctcctg ggccttgccg
     2101 atgaacaaga cgccttcctg tccctgaaac ttcttccggt attcggctgc cacatcgtcc
     2161 ttgcgctgac ccttcccgaa ccggaccacg ggaatgttcc cctgtttggc gaactgttcc
     2221 atttccgcga cgaaggcctt gctgattgga tccatcagcg cactggacgc aaactggtaa
     2281 ccgcgatgga atcggaaaaa gctggctact cccccttccc gctgcaacgt cggcacatat
     2341 acgttcaggt acatccggtc aatgccctca acctccaggc agacatgctc tcgaatcact
     2401 tcggcgacag ttcgtgggat actcattggc ggctccggtt gacaccggcc cttcgggacc
     2461 aaccaccgct tagcggtcca atgaacggaa ttgctcgtca gttcccagct cgaccggagc
     2521 caatcttcgg attatctgat atcgctcaac cgcccgacat acatctcgtc ggtctgaggc
     2581 ggagcctcgt tagctcttac agcaagagga atgctcagta gatacaaaca aaggcatagg
     2641 acgtgcagtt gtgtactctt catcactact gtccaaatta gctgagtaat tcagaatgga
     2701 acaaagtgga gcggtgcttg tagaaggctg agcccctggt aggttgaatt gggttctgga
     2761 agaaccaaaa caaccacag
//
LOCUS       DASRKK010000455         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_133383, whole genome shotgun sequence.
ACCESSION   DASRKK010000455 DASRKK010000000
VERSION     DASRKK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133383"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFC15_10680"
     CDS             <1..394
                     /locus_tag="VFC15_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZM10663.1"
                     /translation="RNTTMSTVNIENPVQAPAPAAKGIQVTDKALARIRLAMVKENIS
                     PTEGGLRLGVQGGGCSGLSYNIRFDTQPRERDRVFQFDDVRVFVDPKSFIYLHGMVLD
                     YEESLMHQGFSFQNPNAKKACGCGTSFS"
     gene            799..1590
                     /gene="hscB"
                     /locus_tag="VFC15_10685"
     CDS             799..1590
                     /gene="hscB"
                     /locus_tag="VFC15_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HZM10664.1"
                     /translation="MALTDHKKPEQLDSGILVVNQSRPTKANCWSCGAMRAAHFCLEC
                     GKVQPPAPVDYFTFFGLPFRLNLEPEKLEREFYALSRHLHPDINAINSDREQQWSLEK
                     SSQLNDAYRTLKDPITRTEYLLRLQGVQLEEQSKTATEEARRTGKIKKQVVPPDLLEQ
                     VFELNMQLEEMRMNKKMGEPDRTLERELQDTKKQLQGKYNDLVEELRTAWNEWDALID
                     RAEEGGGVLDEDRMAVRNKMVDVLNRRTYLRNLLRDIDEVLESRT"
     gene            1794..>2180
                     /locus_tag="VFC15_10690"
     CDS             1794..>2180
                     /locus_tag="VFC15_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM10665.1"
                     /translation="MSTERIVGIDLGTTNSLVAFMEGDRPVVIPGEDGLNLVPSVVAL
                     DENSQIVVGSAARKYLIETPERAVYSVKRLMGRDVEDVRDELKLFPFRLADDLAAGEV
                     LRIKLGDKTFTPPEISAFILRQLKRNA"
BASE COUNT          505 a          582 c          660 g          433 t
ORIGIN      
        1 ccggaacaca accatgagca cagtcaacat tgaaaatccg gtccaggcgc ccgcccccgc
       61 tgccaaaggc atccaggtga cggacaaggc gctggcccgc attcgcttgg cgatggtgaa
      121 ggaaaacatc tcgccaaccg agggcggact acgtctgggt gtgcagggtg gtggttgttc
      181 gggactgagt tacaacatcc gtttcgacac gcagccgcgc gaacgcgatc gcgtctttca
      241 gtttgacgac gttcgcgtct ttgtcgatcc gaagtccttc atttacttgc acggcatggt
      301 tctcgactac gaagaaagcc tgatgcacca gggcttctcc ttccagaacc cgaatgcgaa
      361 gaaggcttgc ggctgcggca cttcgttttc ctaagaaatg gtaacggcgc ccgaatgcga
      421 ccggctgaac gatggggatt ccgcgattgt ggaattccgg ggtaattggt tcgtccgttg
      481 cgatgaggat tatttgtcga gagcggtttc ggagttggtg taaacggcag ttgcggtgcg
      541 gtcttgagga attgtctcgc ggccttgtta gcgttgccac agataaaggt ggtacagaaa
      601 aaccagcccc gcaggggcga aagagcttag cccagcgctt cagcgctggg tagagtggaa
      661 aaaaataacg agtcccttca gggacggcac aaggttcttg gcaggaatag gggtccttcg
      721 actcctcgcg gcgctcgccg ctcaggatga cacagcggaa gaaacattac gctagcgttt
      781 tcagaaagta aggatgcaat ggccctgact gaccacaaga aacccgagca actggacagc
      841 ggcattttag ttgtcaacca aagccggccg accaaggcga attgctggtc gtgcggcgcc
      901 atgcgcgcag cacatttctg tctagagtgc ggcaaagtcc agccgccagc tccggtggac
      961 tatttcactt tcttcgggct tcccttcagg ctcaacctgg agccggaaaa actggaacgc
     1021 gagttctacg ccttgagccg gcacttgcat cccgacatca acgccatcaa cagcgatcgc
     1081 gaacagcagt ggagcctgga gaagagttcg cagcttaacg acgcgtatcg cactctgaaa
     1141 gatcccatca cccggaccga gtacctgttg cgattgcagg gcgtgcaatt ggaagaacaa
     1201 tccaagaccg caaccgaaga ggcgcgccgt accggcaaga tcaaaaagca ggtagttcct
     1261 cccgatctgc tggagcaggt ttttgaattg aatatgcagc tcgaagagat gcggatgaac
     1321 aagaaaatgg gagagcctga ccgcaccctc gagcgtgagc tacaggacac taagaagcag
     1381 ttgcagggca agtacaacga cttggtggaa gagctgcgca cagcatggaa cgagtgggat
     1441 gccttaattg accgggctga ggagggcgga ggagttctgg acgaagatcg catggcagtg
     1501 cgcaacaaga tggtggacgt gctcaatcgc cgcacctacc tgcgcaacct gctgcgcgac
     1561 atcgatgaag tgttggaaag tcgaacttga ctgttcgggt agtgcgggcc ttcaggcccg
     1621 cgtttaggcc gcttattttt tgttgatttg gagtcggctt cagccgacga cagacacagg
     1681 tttgttggcg ggtatagggg tccttcgact cctcgctgcg ctcgtcgctc aggatgacac
     1741 aacacacgga gcgatgcggc taaatgcgaa aggctaaaag cgaaaagcga agcatgtcaa
     1801 ccgaacgcat cgtcggaatc gatctgggca ccaccaactc gctcgtggct ttcatggagg
     1861 gcgaccgtcc cgtcgtgatt cccggcgagg acggtttgaa cctggtgcct tcggtcgtcg
     1921 cgctggacga gaacagccag attgttgtgg gcagcgccgc gcgcaagtat ctgatcgaga
     1981 cgccggagcg cgcggtgtac agcgtgaaac gcctcatggg ccgcgacgtc gaggacgttc
     2041 gcgacgagtt gaagctcttc cctttccgac tggcggacga tctcgcggcc ggcgaagtgc
     2101 tgcgcatcaa gcttggcgac aagactttta ctccgccgga aatctcagct ttcatcctgc
     2161 gccaactgaa gcgcaatgcc
//
LOCUS       DASRKK010000456         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1306788, whole genome shotgun sequence.
ACCESSION   DASRKK010000456 DASRKK010000000
VERSION     DASRKK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306788"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFC15_10695"
     CDS             complement(<1..1676)
                     /locus_tag="VFC15_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZM10666.1"
                     /translation="MRYAFGMPDGEISESSERTNESWWKIPLLYLKGRLQQREDQVFL
                     VLALVIGALTGLAVVAFILLTERAGMRLYPVGGARWRRLLLPVGGSLGIGYLLYRYFP
                     DARGSGVPQTKAALYARGGVITMRTVIGKFFCTSATLASGIPLGREGPSVQVGAGIAS
                     VIGRRLGLRPEKVKALLPVGAAAAIAAAFNTPLAAVLFALEEIVGDLHAPVLGSVVLA
                     SATSWMVLRLLLGNHPLFKVPEYQLVNPLEFGIYAVLGIAGGLVSVSFTKLLLGMRAR
                     FLRFPKKTVWFQPVAGGLLVGMIGLFVPQVLGVGYGYVGDVLNGRMALNLMVLLLVLK
                     LITVTTSYASGNAGGIFGPALFIGAMLGGTVGTAAHHLLPAYTATAGAYALVGMGAVF
                     AGIVRAPMTSVLMIFEMTQDYAVIVPLMIANLVSLFIASRLQPEPIYEALAVQDGIHL
                     PSAETRQRQGKRHIIRIMRALTESLPSEITVGEALQRANSSKFQTWLVLDSNGVVGVI
                     NRATLEREFTQGANHELRKIVGILNFPHVHSDQGLDLALERMGANQIDVLP"
     gene            1891..2337
                     /locus_tag="VFC15_10700"
     CDS             1891..2337
                     /locus_tag="VFC15_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10667.1"
                     /translation="MVVQTRSQVGIIAVGIFLFFGAAMALLAGTTLIWRGTVLDRTWS
                     INAAAYARLAPFGKRIGIPLPLLSAMLAIAGIGWFGRRLWAWRLAVFIIATQVLGDLV
                     NVFMGDVVRGGVGFVIAGTLLFYLLSPEMRSAFERGNASPSVDEFE"
BASE COUNT          507 a          727 c          663 g          493 t
ORIGIN      
        1 ggcaggacgt caatctggtt ggcccccatc cgttccagtg ccaggtcaag gccttgatcg
       61 gagtgaacat gcggaaaatt gagaatgcct acaatcttcc gtagctcatg gttggcacct
      121 tgcgtgaatt ctcgttccag cgtggcacgg ttgatcactc cgacgacgcc gttgctatct
      181 agcacgagcc aggtttggaa tttgctcgag ttggcccgct gcagcgcttc tccaaccgtg
      241 atctcgctcg gcagcgactc cgtcagagca cgcatgattc ggattatgtg acgcttgccc
      301 tgtcgttggc gggtttccgc cgacggcaag tgtatgccat cctgtactgc caaggcctcg
      361 tagatgggct caggctgcag ccgtgaagca atgaagaggc tcaccaaatt cgcaatcatc
      421 agcgggacga tgaccgcgta gtcctgcgtc atctcgaaga tcatcagtac cgacgtcatc
      481 ggagcgcgca cgatacccgc aaagaccgcg cccatcccga ctagcgcgta ggcgccggcc
      541 gtcgcggtat aggcgggcaa tagatgatgg gccgccgtgc ctacagtacc tccgagcatc
      601 gcgccaatga acagcgcagg cccgaatata cctcccgcat tacccgatgc ataacttgtg
      661 gtcacggtta taagcttcag aaccagcaac agaaccatca ggttcagcgc catccgacca
      721 ttcaagacat cgcccacgta cccataaccc acgcccaaga cctgcggcac aaacaatccg
      781 atcatcccaa ctaacaagcc gcccgccacc ggttggaacc aaacggtctt cttggggaag
      841 cgtaggaagc gcgcccgcat gcccagcagg agtttggtga agctcacgga aaccaggccg
      901 ccggcaatgc caagcaccgc gtaaattcca aactccagcg gattgacaag ttggtattcc
      961 ggaactttga agagcgggtg attgccgagc aggagccgaa gcaccatcca tgaagtcgcc
     1021 gaggccaaca ctaccgagcc cagcactggt gcatggaggt cgcccacaat ctcttccagc
     1081 gcaaacagca ctgccgccag cggggtatta aacgcggccg cgatggccgc cgccgcgccc
     1141 accggcagaa gagccttcac tttttcgggc cgcaatccca gacggcggcc gatcacggaa
     1201 gcaatgcccg cgccaacttg aacggacggt ccctcgcggc ccagtgggat tccgctggcc
     1261 agcgttgccg atgtgcagaa aaactttccg atcacggtgc gcatcgtgat gacgccaccg
     1321 cgggcgtaca gcgcagcctt ggtttgcggc acgccgctgc ctcgcgcgtc gggaaaatag
     1381 cggtacagca ggtagccgat tcccagcgaa cctccgacgg ggagcagcag gcgtcgccaa
     1441 cgggcaccgc ccaccggata cagacgcatc cccgctcgtt ctgtcagcag gatgaaagcg
     1501 acaactgcca acccagtcaa cgcacctatt accagcgcca gcaccaggaa tacctggtcc
     1561 tcacgctgct gcagcctgcc tttgagatat aacagcggga ttttccacca actttcgttc
     1621 gtcctttcgc tgctttcgct tatctctccg tccggcatcc caaaagcata ccgcactgcc
     1681 caccccgcaa aaagtctcgg ggcgcatcgt cctccgcccg gaatagacgc ttgggattaa
     1741 tagcgctttc cccagcggac ctcgaatacg tcaaatgtgt ttcaattgtc cgactgacca
     1801 gtccatcaag caggattgag acacgatggt cgcgtggttg tgttgccgga tatagtcatt
     1861 ccttcaggat cgtttggctt cgagttcgaa atggttgttc agacgagatc gcaagtcgga
     1921 atcatcgcgg tgggcatctt cctgtttttc ggcgcagcca tggcgctact cgctggaacc
     1981 acgctgattt ggcggggcac cgttctggac cgaacctggt ccattaatgc ggctgcttat
     2041 gcgcgacttg cacctttcgg aaagaggatt gggattcctc ttccgctgct cagcgcgatg
     2101 ctggccattg ccggcatcgg ttggttcgga cgtcgcctgt gggcttggcg attagcggtg
     2161 ttcatcattg caacccaggt cttgggagat ctcgtgaatg tcttcatggg tgatgttgtc
     2221 agaggaggag tcggcttcgt aatcgccggg accctgctct tttatcttct aagcccagag
     2281 atgagatctg cctttgagcg tggcaatgcg tcgccgtcag ttgacgaatt cgagtagtcg
     2341 aggggcgttt ctgagatgac tccgacctca ggcatagaat ttctggtttg
//
LOCUS       DASRKK010000457        17885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_329757, whole genome shotgun sequence.
ACCESSION   DASRKK010000457 DASRKK010000000
VERSION     DASRKK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17885
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329757"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFC15_10705"
     CDS             complement(<1..257)
                     /locus_tag="VFC15_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HZM10668.1"
                     /translation="MFPIVAARPLARGIKLFEIKAPRIAKKQKPGQFVIVRLDDCGER
                     IPLTIADADPEAGTVTIVVQAIGKTTMLLSMLEAGDSILDVV"
     gene            503..1450
                     /locus_tag="VFC15_10710"
     CDS             503..1450
                     /locus_tag="VFC15_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZM10669.1"
                     /translation="MMPNSSISPVLTTPRRLSPLPYAHWAVASHRVFLGLILLCAHAL
                     TASAQDVSTNPVPSNGFNQTLPDAPSSSRAPESKSFADGVGTVVKTIGQDEWHILKAP
                     FQKKALIWDALVVGATGVLIANDESVAQQVPVSWHQTGINISNACTYGTAAAAGGIYL
                     TGLITHDEHAERTGVLAAEASIDSFLLYGSMKLIFARQRPYTDNADGKFFAGNFSSGS
                     FPSGHAALSWTIASVVAHEYPKLPVQILMYGLATAVATTRVTGGQHFPSDVFVGSTLG
                     YLVGTYVANKDKHVHDPPPHSGNRTKRLPNAILEHVAFQ"
     gene            complement(1571..2125)
                     /gene="nuoI"
                     /locus_tag="VFC15_10715"
     CDS             complement(1571..2125)
                     /gene="nuoI"
                     /locus_tag="VFC15_10715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZM10670.1"
                     /translation="MGIVKNIAAIAKGMSITFGQMFQPTEVENYPDGPGPNRGAMFQE
                     RFRGMHVLQRDENGLEKCVACFLCAAACPSNCIYIEAAENTETNRISGAERYAKVYNI
                     DYNRCIFCGYCVEACPTDAITHGHGFELATFNASSLVYRKEQLLAGTPAHEGSNAIYN
                     IEGTGRLMPKPAQVSSGADVPPGT"
     gene            complement(2191..2787)
                     /locus_tag="VFC15_10720"
     CDS             complement(2191..2787)
                     /locus_tag="VFC15_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM10671.1"
                     /translation="MAQEVFPIPAMTLAIFVKAPLRWRSMLSRPDHTEYADFYANYVS
                     LVPEGSVEEFLLKQWEELVAFMQGVSEDDASVPQAPGKWSIKQVLGHLCDTERVMSYR
                     MLRFARGDQKELHGFEQDDYVAAGEFNTRVRHDLMVEFKNIRGATIALVGSISPEIES
                     RAGAANGNRVSVRALAYIIAGHAQRHLELMHERLNTRT"
     gene            complement(3105..4673)
                     /locus_tag="VFC15_10725"
     CDS             complement(3105..4673)
                     /locus_tag="VFC15_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZM10672.1"
                     /translation="MAYNDLREWISALDKAGELKRIKAEVDPILEIAEITDRVSKWGP
                     RGKHGAGGPALLFENIKGHPGARVLINQFGSKRRMEMALEVDSLDEISGRIKMFMEVK
                     SPQGFLEKLKMLPLLSELGNLFPKVVPTGPCKEVIKKGDFSLLDFPILQCWPMDGGRF
                     ITLPSVITRDPKSGKRNVGTYRMHVYDERNTGMHWQRQKQGAEHYRNRMRKLVAEGAE
                     NPVTAALDLMARSGGGSRVPKGKQVAGRMDVAVAIGTDPAMMFSSIVPAPPDVEEFII
                     AGFLRQKSVELVKCETVDLEVPANSEIVLEGYVELDELKMEGPFGDHTGFYTMEDLYP
                     VFHITCITHRKDPIYSTTIVGKPPMEDAWMGKAVERIFLPLMKLTIPEIVDINLPIEG
                     VFHNLMIVSVKKSYPGQARKVMNAIWGLGQAMSTKCILVVDEDVNVQDLAEVTLKVLN
                     NIDPERDIQFTLGPVDSLDHASRLPDYGSKMGVDATRKWPSEGFTRPWPEEITMDAET
                     KRRVTERWKEFGIE"
     gene            complement(4919..6667)
                     /locus_tag="VFC15_10730"
     CDS             complement(4919..6667)
                     /locus_tag="VFC15_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10673.1"
                     /translation="MADYIYSMESRLTPEQQRGVNLVQEIARHAGLNLYLTGGTIRDL
                     ITGFPIRDIDLSIQGNPLKLQKELEKAGISVQGVDEDLKTLHLLMPGNVRAEINLTRS
                     ETYDKPGRPPVISQATINEDLRRRDFTVNAMALSLNPGSRGLLLDPFNGIADIEAKVL
                     RVLHNYAFLEDPVRLLRATRLSARFHWPLEERTQARYDSAKENNYIEYVGKRSIGAEL
                     EQLAHEDDPISIMRAMEKDGWLKVLHPHWTVAKAEASDLGHAIKLRQTLTDLGYSIES
                     GPMVMHFLTRKMSDKDISDIQRMVPRRDFVEKWKHLEHNAHDLAKKLTGKEAATNSGA
                     WKILSAAQPENILFLSLTTKQQAVDQKLKNFLTKWRQVREKLPYAEMAEMRITAQLPE
                     YQKILDEAFLLLLDGKLRSHNEIVNFLKPYEPPPPPPPPPPKRGRGKAAAVEAAAGVP
                     ADVVAAPAKRGRKPKGAAAVAQVAPVTASAVPAEQAKTTAGAKPVAPAKPAPEAKKAA
                     PVKGTSNPAALKAAPAKTAKKLDKLAKKAVPAKKKSAVTKTPTPAKKKVAAKKVPVKK
                     PASKKPAAKKAAKKKH"
     gene            6921..7445
                     /locus_tag="VFC15_10735"
     CDS             6921..7445
                     /locus_tag="VFC15_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10674.1"
                     /translation="MRQSDVPSALTAYLINMATTHKKVVVRKMDRDMVSGYVSAAFIS
                     DGKIEVLNTAGNVVAIDLREVKGVYFVREFGDTESLARKTFTSRPRTEGLWVRLRFRD
                     NEIIEGMMINDLVQDGTEGFSIIPPDTRSNTQRIFVPRSALAEMTVLAVIGEKKSQRR
                     GRHPDDTLQVPMFN"
     gene            7551..8582
                     /gene="lpxD"
                     /locus_tag="VFC15_10740"
     CDS             7551..8582
                     /gene="lpxD"
                     /locus_tag="VFC15_10740"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZM10675.1"
                     /translation="MKLGQIASTLGATLENGDPETEITGVSGIEEAVAGQITFVANPK
                     YFAAAKNTSASAIIVSEDFPSVACVLLRSQNPYLCFARAIDLFYQSPRYKSGIHPTAV
                     IAPTARIGSNAHIGAYVVIDDGVELGDDSVLLPHVVIYRGAKIGSNFLAHAHSIVRED
                     CRIGDNVILQNGAIVGCDGFGFARDGDHWEKISQSGPTVIESDVEVQANACIDRASVG
                     ETRIARGVKIDNLTQVGHGAAVGEHTLLCAQVGLAGSTVVGKNVIIAGQAGVAGHCNV
                     GDGVVITAQSGTHGDIPAGSMVSGSPAFEHKQWLRAVNAFSKLPEMAKAVRNLTQKQS
                     NAAAGKNEE"
     gene            8678..9484
                     /locus_tag="VFC15_10745"
     CDS             8678..9484
                     /locus_tag="VFC15_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM10676.1"
                     /translation="MSEDIPIPVSNPVLEPHMVPVRPIRVLLLDDREENLVLRSAILR
                     QKGYQVVTTRSIEQAQARLHEIDIAVLDYHLGAGKFGTAVAKTLRRDRPQVPIIVLSA
                     TLDRKFGGIADMHLLKGHSSVEDLLGALRSFEAKKRGRPVVVDARDFYYSRISMAMGD
                     DIVLEILDRDGNWQFVNDYFAAMRGKKRGWFAGKNLFDHFPELGEEWRDIVRTVVDTR
                     ETYIDRRGFGGTAHLPAKNAHWMWNVLILPIKLHDNRDGAVLSARMIEKK"
     gene            9545..11242
                     /locus_tag="VFC15_10750"
     CDS             9545..11242
                     /locus_tag="VFC15_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10677.1"
                     /translation="MNNPFANRTTAERLELEQRYARQRRDGWFTLALGIVSIAALLWY
                     YAHQQVLLYGDAVAHVNIARHVLDNRSWLSSFFQLGTVWLPLPHILMLPFVWNKALWM
                     SGIAGSIASMVAYVAGALGIFRLVSARAPQAAAYVAAAIYALNPNLLYMQTTAMNEPL
                     FLAFFIWAIVYFDEWLRALSTTPPAMASDHSPERALEGCGIALAGGAATRYDGWFFGA
                     IMGALVLWAFARWWRNTPDAARRRGMSKSLAEFLLLNALVPVFWLAYNHRVSGRAMDW
                     ANGPYSARAIAERTTTRGAAPYPGKDHVVTAGMYFLKAAKLNVGPGHWGDVLFLLAVL
                     GTAVALWRFRQCRTWLLLWVPLTFYALSIAYGSVPIFIPTWWPFSYYNVRYGLELLPV
                     FAVFPALLAVFLAEFIANPKKKLVVWSLLALLVTASYLSIYADEPITLKEAQVNSRTR
                     MILEDELAKFLVALPPSSTLLMYQGEHVGALQRAGIPLRHVISEVSHPDWEWALLDPA
                     HRADFIIAFKGDPVWMAAHEHRQELTELISITVPGQAKCTVYRPSRRDGGPALPAAQ"
     gene            11317..12099
                     /gene="dapF"
                     /locus_tag="VFC15_10755"
     CDS             11317..12099
                     /gene="dapF"
                     /locus_tag="VFC15_10755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZM10678.1"
                     /translation="MSSIPFVKATACGNDFLIIGIEHAPADIAGFSRLICDRHNGVGA
                     DGVEWVVPASDADVAIRLLNADGSEAEISGNGTRCVAARYCAEHHRTSVVIRTGAGLK
                     TCDLTRRDGHNFEFRTAMGQPEVSREMEVRLTNEIVRGIPVSMGNPHYVMFVREFQPE
                     WQTWAAQIATHPDFPYGTNVEVVRVLNDHEIEVRFYERGVGETMSSGTGSSAAAVASI
                     HAGKVTSPVLVHAPGGIQTVEWDREVILQGSATILCRGDFFV"
     gene            12114..13088
                     /locus_tag="VFC15_10760"
     CDS             12114..13088
                     /locus_tag="VFC15_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZM10679.1"
                     /translation="MATFTQSERLRPAALRPGDTIGIIAPAGPIEPDAMEAGCAWLRD
                     RGYQPFYLPSILDRDSYFAGSTARRLDEFHQMFARPEVKAIICARGGYGCNYLLPHID
                     LNVVQANPKIFCGCSDVTTLLTYLCDAARMVTFHGPMLNIDVRPDGVDEESWAAAMTS
                     GEAYQMEFGNDEVQTLVPGSAEGMLYGGCLSLLCASLGTPYEIETRGTILFLEDLAEP
                     AFRIDRMLMHLKLAGKFDGVRGVIFGEMLNCGPRDPQDYSLQQVVQRVLGDLGVPMAY
                     GLKSGHVSRGNITLPLGVATQFTAGANVSLRFEAAVKREFAKVPVSRP"
     gene            13085..14509
                     /gene="mpl"
                     /locus_tag="VFC15_10765"
     CDS             13085..14509
                     /gene="mpl"
                     /locus_tag="VFC15_10765"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZM10680.1"
                     /translation="MKQKHIHLIGICGTAMASLAGMLKQRGHHVTGSDVAAYPPMSDF
                     LASLGIPVSQPFSEKNLEPRPDLVIVGNAISRGNVELEYALDQRLAYQSMAHVVHEEF
                     LVDRERLVVAGTHGKTTTSSLLAWQFTVALRAPGFLIGGIPENFGSSFALGNGPEFII
                     EGDEYDTAFFDKGPKFLHYFPDAVILTSVEFDHADIYKDLDEVKTAFKRLVNLVPRRG
                     LLIAWDGHPNVDECVSRALCRVERYGFGEGSAWRISEANYGDSLTRWKVARDGAPWAE
                     FESQLAGEYNILNATAAAAMAANYGIPVEVIAKALRSFRSVKRRLEVKAEIHGITIID
                     DFAHHPTAIAGTLKALRERYRGRRLWVVLEPRSNTLRRKVFENELAGSLAFADQVVLA
                     GVFKSDAIPQAERLDPQKVITKLNLAGTPARLLASADAIVDAIALELCEGDVVAILSN
                     GGFGGIYEKLPAKLKSLHEASTPA"
     gene            14509..16380
                     /locus_tag="VFC15_10770"
     CDS             14509..16380
                     /locus_tag="VFC15_10770"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM10681.1"
                     /translation="MHPGKTALAILRLGFFVAVASAAQLWGATPVTCSSPVDSRPQAT
                     EYFVSLADNAHHLAHVSIRFPQTSGAIALKMPVWDALYQVRDFAANVQKVRAADVSGR
                     PVSVVNSKTSEWKVTAPGPCVVVDYDIRLDNDGPFGAQLNAEHGFFNWAMVLMYSPAT
                     RSQSMSLQLLDVPAAWGLRDVHILGGAPAGQVEQAVGVATGYDELVDSPAEVGSFQQF
                     EFQKDGVTYHIVVHGDPRDYDGGKLQDIVRKITHAAVEWMADRPFDDYTFLYHFPRSP
                     GHGGMEHAYGTAIDINAERLHNSMTPVASVSAHEFFHLWNVKRIRPQSLDPIDYQRAM
                     DTRALWFSEGVTSTVGDMLLARAGLLSDRQYIDRVASEINELQVRPAHRWQSVEESSL
                     DAWFEGDAFYRSPERSISYYNKGEVLGVLLDLRMRELTQGGKSLRDLFHWMNENYAKK
                     NKPFPDSDGVQQAAETITGQSFAEFFHNYVAGVLELPYNEYFQFVGLQVAETTVRTST
                     PGFTTSTNPGGQPEVASVDANSDAQRAGVAIGDHVVEVDGRPATGSLDDQLSRMRAGV
                     TVKLNFSNRRGKRTIKLKLTSRQEQMYILQDVPPVTPEQLAHRVAWIHGDDESAGGQ"
     gene            16377..17084
                     /locus_tag="VFC15_10775"
     CDS             16377..17084
                     /locus_tag="VFC15_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM10682.1"
                     /translation="MIRTSFMLTFWGLSLLITGPFLLLYTFLSGNIMPLYRIANVLAI
                     TGVRMAGVKIEVRGLENLKSGCNYIFMSNHVSNLDPPVLLPSIPCRCSVLVKKEVFRI
                     PILGTAMKMAELVPVDRSNREAAVESVRAGADVMRHGLSMLIFPEGTRSPDGRLLPFK
                     KGPFHLAMESSVPVIPVTLLGTSYCWPKSTWAMRQGNVTLIFHPPIDPSGFTDREDLM
                     AAVRQSIASALPEDRRD"
     gene            complement(17099..>17885)
                     /locus_tag="VFC15_10780"
     CDS             complement(17099..>17885)
                     /locus_tag="VFC15_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZM10683.1"
                     /translation="VVGKDFTAEHEAVMNKRGVDTPGIQHADGKTFFWAGDYGDNVNE
                     AQTLQTDLNVFASFQPQIPAEYQNSDFLFLANIDPVLQAQVRDAMPNVKLVGGDTMNY
                     WIKDHRQNLGEMLKRVDVLLINDTEAKMLAGDSSLPRAARKVLALGPKALVIKHGEYG
                     ATIFFRDGEFAGGHHPFRAPALPLDEVRDPTGAGDSFAGGFMGYIASQGEVTRNVLKH
                     AMFYGSVMGSFSVEQFGTERLQSLTREEIDQRFSVFRELTHLD"
BASE COUNT         3655 a         5189 c         5149 g         3892 t
ORIGIN      
        1 accacatcca ggatggaatc tccggcttcc aacatgctaa gcagcatcgt ggtcttgccg
       61 atcgcctgga cgacgatggt gacggttccg gcttcagggt cagcgtcggc gatggtaagt
      121 ggaatgcgct cgccacagtc atcgagccgc acaatcacga attggccagg tttctgcttc
      181 ttcgcgatcc gcggcgcctt gatttcaaac agtttgatgc ctcgcgccag ggggcgagct
      241 gcgacaattg ggaacacagg aaatcctccc acctttgatt ctgcgggggt taagcacttg
      301 agtctgtggc atacgtcaca ataagggcgg acatagcgca caacaaaacg ccggcactgc
      361 cgattatcgg caaaacgccg tattgtcgcg gtttgagcga accttttctg acacaattgg
      421 gttacgaatt tgcagagtta gaggcctcat gaccaccacc gccatcccgc aagagacaag
      481 atcggggcca gcaagaacgc ttatgatgcc aaactcatct atctcgccag ttttgacgac
      541 gcctcgtcgg ctctctccgc taccttacgc gcactgggcc gttgcctcgc atcgggtttt
      601 cctcggcttg atcttgctgt gcgcccatgc tctgaccgct tcagcgcagg atgtctcaac
      661 caatccagta ccgtcaaatg gtttcaatca gaccctcccg gacgcgccgt catcttcacg
      721 cgcacccgag tcgaagtcgt tcgccgacgg cgtcgggacg gtggtcaaga cgattggcca
      781 ggatgaatgg cacatcctca aggcgccctt ccagaagaag gccctcattt gggacgcgct
      841 cgtggtgggc gcgactggcg tgctgatcgc caacgacgag tcggtggcgc agcaggttcc
      901 ggtcagttgg caccagacgg gtatcaacat ctccaacgcg tgcacctacg ggacggcggc
      961 ggcagccggc ggcatctacc tcaccgggct aatcacccac gatgagcacg cggagcgcac
     1021 tggcgtgctg gccgccgagg cgtcaatcga cagttttctt ttgtacggat cgatgaagtt
     1081 gatcttcgcg aggcaacggc cttacacgga caacgccgat ggaaagttct ttgccggcaa
     1141 cttcagtagt ggttcattcc cttccgggca cgccgcgttg tcatggacca tcgcctctgt
     1201 ggtggcccat gagtatccaa agctgccggt gcagatcctc atgtacggac tggcgaccgc
     1261 tgttgcgacg actcgcgtta caggcggaca gcacttcccg tccgatgtgt tcgtgggatc
     1321 gacgctgggc tacctcgtcg gcacgtacgt ggccaataaa gacaaacatg tccatgaccc
     1381 gcctccacac tcgggaaacc gaacaaagcg gctgcccaat gccatcctgg agcacgtcgc
     1441 gtttcagtag cgacacactc tacgcaacaa aggatttcgc cgcggagtgg tacaccacga
     1501 tcactgacgc gttctgctcg gggtggggca ggaagccggt cgcgagttgc agcccgatgg
     1561 tttggtcgcg ctacgttccg ggcggaacat cggcgcctga actcacttgc gcgggcttcg
     1621 gcatcaacct gccggtgcct tcgatgttgt agatggcgtt cgagccttcg tgcgccggag
     1681 tccccgccag caactgttcc ttgcggtaaa ccaagctgct cgcattgaac gtcgccaatt
     1741 cgaagccatg cccgtgagta atcgcgtcgg tcgggcaggc ttccacgcaa tagccgcaga
     1801 agatgcagcg gttgtaatcg atgttgtaca ccttagcgta gcgctcggcg ccgctgatcc
     1861 ggttagtttc ggtgttctcc gccgcttcaa tgtaaatgca gttcgagggg caggcagccg
     1921 cacagaggaa gcaagcaaca catttctcca acccgttctc gtcgcgctgc aggacatgca
     1981 tgccgcgaaa acgctcctgg aacatggcgc cacgattggg ccccggaccg tcgggatagt
     2041 tttctacctc cgtgggttga aacatctgtc caaaggtgat gctcatgcct ttggcgatcg
     2101 cggcgatatt tttgacgatt cccatacgag aagacagtat aacaatccgg ctcgggtgtt
     2161 gggtacagcc tgaagtggcc gccgcgaaga ttacgttcga gtattcaatc gctcgtgcat
     2221 cagctccaga tgacgctgag catggcccgc gatgatgtac gccagggccc gcacggatac
     2281 acgattgccg ttggcggcgc ctgctcggct ttctatctcg ggtgaaatgg agcccaccag
     2341 cgcgatggtc gctccccgga tgtttttgaa ctcgaccatg agatcgtgcc gcacccgagt
     2401 gttgaactcg ccggcggcga cgtaatcatc ctgctcgaaa ccgtgcagtt ctttctgatc
     2461 gccacgggca aagcgcagca tccggtagga catgacgcgt tcggtgtcgc acagatgacc
     2521 aagcacctgc ttgatactcc atttgcccgg agcctgcggc acgctggcgt cgtcttcaga
     2581 gacgccctgc atgaaggcaa ccaactcttc ccactgcttc agcaggaact cctcgaccga
     2641 tccttcagga acgagggaaa cataattggc atagaaatcg gcgtattcag tgtggtcggg
     2701 acgtgaaagc attgatctcc acctcaaagg tgctttaaca aagatggcta gcgtcatcgc
     2761 gggtatagga aagacctctt gcgccacaac ttccgcatcg cgagcgataa gccagactta
     2821 tcgggattgc aagggacaaa aatttatgga tcttctggtt gaaaggcatg ccgcaattag
     2881 gacagcgcca gttcgcgctg aggtatcccg aaaaaacaaa aatgccgacg taaaaccagg
     2941 ccagaacgag ccataggcgc ggacccaaga gcctggctcc caatccgact attggcagaa
     3001 aaaccaaaaa caaaactatc ggcaaagaca agatgatccg caaattgcgc cagcgctttc
     3061 tcacatcttc ggaaccttgc gaaatcattc gagaactctc cagcttactc tatcccgaat
     3121 tccttccacc gctccgtgac tcggcgcttg gtttcggcat ccatcgtaat ttcttcgggc
     3181 cacggacgcg tgaatccctc actgggccat ttgcgggtag cgtccacgcc catctttgag
     3241 ccgtagtccg gcaggcggga cgcatgatcg agcgaatcga ccggccccag cgtgaactgg
     3301 atatcgcgct ctgggtcgat attgttcagc accttcagcg tgacctcggc tagatcctgc
     3361 acgttcacgt cttcatcgac gaccagaatg cacttagtgg acatggcctg ccccagtccc
     3421 cagatcgcat tcatcacttt gcgagcctgc ccgggatacg atttcttgac ggagacgatc
     3481 atcaggttgt gaaacacgcc ttcgatgggc aggtttatat cgactatctc ggggatggtg
     3541 agcttcatca gcggcaggaa aatacgctcg acagctttgc ccatccacgc gtcttccatg
     3601 ggaggcttgc ccacgatggt ggtcgagtag atcgggtcct tgcggtgcgt aatgcaagtg
     3661 atgtgaaata ccgggtaaag gtcttccatc gtgtagaagc cagtgtgatc gccgaagggg
     3721 ccttccatct tcagttcgtc caactcgacg tagccttcga gaacgatctc cgagttcgcg
     3781 ggaacttcga ggtccaccgt ttcgcatttc accagttcga ccgacttctg ccgcaggaac
     3841 cccgcgatga tgaactcttc gacatcgggc ggagcgggca cgatggacga gaacatcatc
     3901 gcgggatcgg tgccgatcgc gaccgcaaca tccatacgtc cagcaacctg cttgcctttg
     3961 ggaacgcgcg atccaccacc ggagcgcgcc atcaaatcga gcgccgcagt cacgggattc
     4021 tccgcgccct cggccaccag cttgcgcatg cggttgcgat aatgctcggc accctgcttc
     4081 tgccgttgcc agtgcatgcc cgtgttgcgt tcgtcgtaaa cgtgcatgcg atacgtaccc
     4141 acgttgcgct tacccgactt gggatctcgg gtgattaccg atggcagcgt gatgaaccgt
     4201 ccgccgtcca ttggccagca ttgcaggatg ggaaaatcga gtagcgagaa gtcgcccttc
     4261 ttgatcactt ccttgcacgg gccagtgggg actaccttgg gaaacagatt tcccaactca
     4321 gagagcaagg gcagcatctt tagcttttcg agaaagcctt gcggcgactt cacctccatg
     4381 aacatcttga tgcgcccaga aatctcatcc agcgagtcga cttccagcgc catctccatg
     4441 cgtcgtttgg atccgaactg gttgatcaag actcttgcgc cgggatgtcc cttgatgttc
     4501 tcgaacagaa gcgccggacc gcctgcgcca tgtttaccac gcggccccca cttcgatacg
     4561 cggtcggtga tctcagcgat ttccaggatg ggatcgactt ccgctttgat gcgctttagc
     4621 tctcccgcct tgtccagcgc ggaaatccat tccctcaagt cgttataggc cactagccag
     4681 cctctcggca gcggcaggca ccgctgcaac cctcttcaat atgagtatgc aacaaccaca
     4741 attatagaag cggcagttat tgaccgggtc aacgctccat cctcatgcgg caatgagttt
     4801 gcgattgccg caaaaaatca gatcggtgcg ttggtccggc gtgcaataag aaaggcgcac
     4861 cagattacgg cacgcccatc gaaatcaact gcgacctgcg accgccaagt cagcccggtc
     4921 aatgcttctt cttcgctgcc tttttcgccg ccggtttctt ggacgccggt ttcttcaccg
     4981 gaactttctt cgctgccacc ttctttttcg cgggcgtcgg tgtctttgtc acggcagact
     5041 ttttctttgc cggcacagcc ttcttggcca gtttgtccag cttcttcgcg gtcttggctg
     5101 gggcagcttt aagcgcggcc gggttagacg tgcctttcac cggagcagcc tttttcgctt
     5161 ccggcgcagg tttcgccgga gccacaggtt tcgctcccgc ggtggtctta gcttgttccg
     5221 cagggaccgc cgacgccgtt acgggagcca cttgcgccac ggcagcagca cccttcggct
     5281 tgcgtccacg cttagctgga gcagcgacca catccgcagg aacacccgcg gcagcttcaa
     5341 cagcagcggc cttgccccgt cctcgcttcg ggggcggtgg tggtggcggt gggggaggct
     5401 cgtaaggctt caggaaattg acgatctcgt tgtgcgacct tagttttccg tcgagcaaga
     5461 gcagaaaggc ctcatccaga atcttctggt actccggaag ctgagcggta atccgcattt
     5521 cggccatctc tgcgtacggc agtttttccc tcacttgccg ccacttggta agaaagttct
     5581 tgagcttctg atcaaccgcc tgttgcttgg tggtcaggct cagaaacagt atgttctcag
     5641 gttgcgcagc actcagtatc ttccacgctc cagaattcgt ggcagcctct tttccagtca
     5701 acttcttggc gagatcatga gcgttgtgct cgaggtgctt ccatttttcc acgaaatcgc
     5761 gacgcggaac catacgttgg atatccgaaa tatccttgtc gctcatcttg cgagtgagga
     5821 agtgcatcac catcggcccg gattcaatcg agtatcccag gtccgtaaga gtctgccgca
     5881 acttgatggc gtgtcccaga tcgctggcct cagccttggc cacggtccag tgcggatgca
     5941 gaaccttcag ccaaccatcc ttctccatgg cacgcatgat gctgatgggg tcgtcttcat
     6001 gtgccaactg ctccaactca gcaccgattg agcgcttacc aacgtattcg atgtagttgt
     6061 tctcttttgc cgagtcgtaa cgggcctggg tccgttcttc caacggccaa tggaaccgtg
     6121 cgctcaatcg agtggcgcgc agcaggcgga ccggatcttc cagaaacgcg taattgtgca
     6181 gaacccgcag aactttagcc tcgatgtcgg caatgccatt gaagggatcg agcagaagtc
     6241 cccgcgatcc tggatttaac gaaagtgcca tggcgttaac ggtaaagtcg cgtcgccgaa
     6301 ggtcttcgtt gatggtcgcc tgactgatga ccggaggcct gccgggttta tcgtaagtct
     6361 ctgagcgggt gagatttatc tcggctcgca cattgcccgg catgagcaag tgcagggtct
     6421 tcaagtcttc gtcgacaccc tggacggaga tcccagcttt ctccaattct ttctgtaact
     6481 taaggggatt accctggatg ctcagatcga tgtcgcggat gggaaatccg gtgatcaggt
     6541 cccgaatcgt gccgccggtg aggtaaagat tcaatcccgc gtgccgtgcg atttcctgta
     6601 ccagattcac accgcgctgc tgctcagggg tgagcctgga ttccatgctg taaatgtagt
     6661 ctgccatcta tatgtttgac tctaaaggtg atagccgaag atgatcagga agcgcgcgga
     6721 gtatgcctca cggcgaaatc cctgtttcac gggtcgctcg gccgccgcac tcgccgtcct
     6781 taccgccaga cggagcctgc aacttgctct tagggcgaca cttacagcac aatgcaccaa
     6841 gtttactgca tagcgacaat aacacaagga taacgatttg gctacaaact atgttactta
     6901 gcggcgcttt ttcctcaaaa gtgcgacaat cggacgtgcc gtcagcactg acggcttacc
     6961 tcatcaacat ggccaccacg cacaaaaaag tagttgtccg gaagatggac cgcgacatgg
     7021 tcagcggcta cgtgtcggct gctttcatct ccgatggcaa gatcgaggtg ctgaacaccg
     7081 ccggcaacgt ggtggccatc gatctgcgtg aggtcaaagg tgtgtatttt gtgcgcgagt
     7141 ttggggacac cgagtcgctc gctcgcaaga cgttcacttc ccgtccgcga acggaggggt
     7201 tgtgggttcg tctgcgcttt cgcgataacg aaatcatcga aggcatgatg attaacgacc
     7261 tggttcagga tggcactgag ggattctcca ttatcccgcc cgatacgcgg tccaatacgc
     7321 aacgcatttt cgtaccccga tcggctctgg ccgagatgac tgtgctggcc gtaatcggcg
     7381 agaagaaatc gcagcgacgc ggacggcacc ccgacgacac gcttcaggtg ccgatgttca
     7441 actgatggtg tcgctcggac ccagcgagcg tcgaggcgta tccgggagcg cgaggttcac
     7501 ctctggccaa aaatagaccg agccgagctc gggtttcctg accgaagatt atgaaacttg
     7561 gccaaatcgc aagcacgctt ggcgccacac tggagaacgg cgatcctgaa accgaaatta
     7621 caggtgtgtc tgggatcgaa gaagccgttg ccggccagat taccttcgta gctaatccga
     7681 aatacttcgc cgcagctaag aacaccagcg cttcggcgat cattgtttcc gaggacttcc
     7741 cttccgtagc atgcgttttg ttgcgaagcc agaatcctta cctctgcttt gcgcgtgcca
     7801 tcgacctgtt ttaccagtct ccacggtata aatcaggaat ccatcccacg gcagtgatcg
     7861 cgccgacggc caggatcggg tccaatgcgc acattggagc ctatgtggta atcgatgacg
     7921 gcgtagaact cggcgatgat tcggttcttt tgccgcatgt cgtcatctat cgtggagcga
     7981 agatcggcag caacttcctg gcccacgctc actccatcgt gcgtgaagac tgccgaatcg
     8041 gagacaacgt tattcttcaa aacggagcga tcgtaggttg cgacggattc ggttttgctc
     8101 gcgatggcga tcactgggaa aagatttcgc aatcgggtcc aaccgttatt gagagcgatg
     8161 tcgaagttca agccaacgct tgcatcgatc gtgccagcgt cggcgagacc cgcatcgcac
     8221 gtggcgtgaa gatcgataac ctgacccagg tcggacacgg cgctgcagtc ggcgaacaca
     8281 cgttgctgtg cgcccaggta ggtctggccg gctcgaccgt ggtgggcaag aacgtcatca
     8341 tcgctggaca agcgggtgtt gccgggcact gtaacgtcgg ggacggcgtg gtcattaccg
     8401 cgcaaagcgg gacccacggc gacattccgg cggggagcat ggttagcgga tctcccgctt
     8461 ttgagcacaa gcagtggttg cgggccgtca atgcgttcag caaactcccg gaaatggcga
     8521 aggcggtccg caatctcaca caaaagcaat cgaacgcggc ggccggaaag aacgaagagt
     8581 aacctggcgc cgctcgtcta acattttgta gtagtttgca tttttcgaga tgcttgaggt
     8641 ccaccgcagt ttgagtgcgt tctccaccca ctaaaacatg agcgaagata ttcccattcc
     8701 cgtctcgaac ccggtactgg aaccgcacat ggttccggtt cggccgattc gcgttttgct
     8761 gctcgacgac cgcgaggaga atctggtctt gcgttccgca attcttcgcc agaagggcta
     8821 ccaggtagtt acgaccagat ccatcgagca ggcccaagcc aggttgcacg agatcgatat
     8881 cgctgtcctc gattatcatc tcggcgcagg aaagtttggg actgctgtcg cgaaaacgtt
     8941 acgccgagac cgcccgcagg ttccgatcat cgtgttgtcg gccactctcg atcgcaagtt
     9001 cggcggtatc gccgatatgc acctgctcaa ggggcacagt tcggttgaag atctgctcgg
     9061 cgcgctacgt tcgttcgagg ccaagaagcg tggccgtccg gtggtggtcg atgcgcgtga
     9121 tttttattat tcacgcatct cgatggccat gggcgacgac attgtgctcg aaattctcga
     9181 ccgcgacggc aattggcagt ttgtgaatga ttactttgcc gcgatgagag gcaagaagcg
     9241 cggctggttt gccggcaaga acctgtttga tcattttccg gagctgggcg aggagtggcg
     9301 cgatatcgtt cgcaccgtgg tcgatactcg cgaaacctac atcgaccgtc gcggcttcgg
     9361 cgggaccgcg catcttcccg ccaagaatgc ccactggatg tggaacgtgc tgattctccc
     9421 catcaagctg cacgacaacc gagatggagc cgtcctcagc gctcgcatga tcgagaaaaa
     9481 ataggtccag aacaggatcg tgcacacctc tttttctcaa cctctcatgt cgtagactga
     9541 attcttgaac aatccatttg caaataggac caccgcggag cgcttggaac ttgagcagcg
     9601 ttatgcgcgc cagcgcaggg acggctggtt cactctcgcg ctcgggatag tctcgatagc
     9661 ggcgctgctg tggtactacg ctcatcagca ggtcctgctg tatggcgacg cggtggcgca
     9721 cgtcaacatc gcgcgccacg ttctcgacaa ccgctcctgg ctctcgtcgt tctttcagct
     9781 gggcacggtc tggctgccgc tgccgcacat cctgatgcta cctttcgttt ggaacaaggc
     9841 gctgtggatg agcggcattg caggttcgat cgcgagcatg gtggcgtatg tggctggcgc
     9901 gctcggaatc ttccgcctgg tcagcgctcg agcaccgcag gcggcggcat acgttgcggc
     9961 ggccatttac gcactcaacc ctaacctgct gtacatgcaa accacggcga tgaatgagcc
    10021 gctgttcctc gcgttcttca tctgggccat cgtctatttc gatgaatggc tgcgcgcttt
    10081 atccacgaca ccgcctgcga tggcttcgga ccacagtccc gaacgtgcgc tcgaaggctg
    10141 cggcattgcc ctggccggag gggcggcgac gcgatacgac ggctggtttt tcggcgcaat
    10201 catgggcgca ctcgtgctgt gggcgtttgc acggtggtgg aggaacacac ccgacgcggc
    10261 gcgccgccgc gggatgagca aatctctggc ggaatttctg ctgctcaatg cactggtgcc
    10321 ggtattctgg ctcgcgtaca accatcgcgt ttcaggacgc gccatggact gggccaacgg
    10381 cccgtattcg gccagagcta tcgccgaacg caccaccacc cgcggcgctg ctccgtatcc
    10441 cggcaaggac cacgttgtga cggccgggat gtacttcctg aaagcggcca agctcaacgt
    10501 cggccccgga cactggggtg acgtgctttt cttgctggct gttcttggaa ctgcggttgc
    10561 actttggcga ttccgtcaat gccgcacatg gctgctgctc tgggttccac ttacgttcta
    10621 tgcgttgtcg attgcatacg gcagcgtgcc tatcttcatt ccaacctggt ggccgttttc
    10681 gtactacaac gtgcggtatg gcctggaact cctgccggtt tttgcagtgt ttcctgcgct
    10741 gctagccgtg tttctggcgg agttcatcgc aaaccccaaa aaaaagctcg ttgtctggag
    10801 tttgctggcg ctgctggtga cggccagtta tctgtcaatc tatgccgacg aacccatcac
    10861 gctgaaggag gcgcaagtta actcccgcac aaggatgatc ctcgaagatg aactagcaaa
    10921 gttcctggtg gcgctgccac cctcgtccac gctgctcatg taccagggtg aacacgtagg
    10981 cgcgctgcaa cgggccggca ttccgttgcg gcatgtgatc agcgaagtga gtcatccgga
    11041 ttgggagtgg gcattgctcg acccagcaca ccgagctgat tttattatcg ccttcaaggg
    11101 ggacccggtg tggatggcag cgcacgagca tcgccaggaa ctgacagagc tgatttcgat
    11161 caccgtacct ggtcaggcca agtgcaccgt ttatcgaccc agccggcgag atggtggacc
    11221 ggcattgcca gcggcacaat agcggcgttc cgtcagggac gctagtttcg acgctttgct
    11281 acaatccgcg cagtgacgat gcatgcttca aaccccatgt cttccattcc ttttgtaaaa
    11341 gctacagcct gtggcaatga ttttctaatc attgggatcg agcacgcgcc tgcggacatc
    11401 gctggattca gtcgactcat ctgcgatcgc cataacggcg tcggtgcaga cggagtcgaa
    11461 tgggtcgtgc ctgcctccga tgctgacgtt gcgattcgct tgctaaatgc cgatggctcc
    11521 gaagcggaga tctccggcaa cggtacccga tgtgtggccg cgcggtattg cgccgagcat
    11581 caccgcacct ccgtagtcat aaggacgggg gcgggactca agacctgcga tctcacccgt
    11641 cgcgacggac acaattttga gtttcgaacg gcgatgggcc agcctgaggt cagccgcgaa
    11701 atggaggtcc ggcttaccaa cgaaatcgtt cgcggcatcc cggtgtcgat gggcaatccg
    11761 cactacgtga tgtttgtgcg ggaatttcag cccgagtggc agacctgggc tgcgcagatt
    11821 gccacgcatc cagacttccc gtatggaacg aacgtcgagg tggtcagggt gctcaatgat
    11881 cacgaaatcg aagtgcggtt ctacgaacgc ggggtcggcg agacgatgtc gtcgggaaca
    11941 ggttcgtcgg ctgccgccgt ggcttcgatt catgccggca aggtgacgtc tccggtgctg
    12001 gtgcacgccc ccggaggcat acagaccgtg gaatgggacc gcgaagtaat actgcaaggg
    12061 tcagcaacga tactctgccg cggtgatttt ttcgtttaag atagtccttc cgaatggcaa
    12121 ccttcaccca atctgagcgc ctgcgacctg ctgccctgcg gcccggcgac accatcggca
    12181 tcattgcgcc ggcaggtccg attgagcccg acgccatgga ggccggttgc gcctggctgc
    12241 gtgaccgggg atatcagcct ttctatctgc cttcaattct cgaccgcgat tcatatttcg
    12301 ccggaagcac agcgcggcgt ctcgatgagt tccatcagat gtttgcgcgt ccggaggtga
    12361 aggcgatcat ctgcgctcgc ggcggatacg gttgcaatta cttactgccg cacatcgacc
    12421 ttaatgtggt acaggcgaat cccaagatat tttgcggctg cagtgacgta accacgctgc
    12481 tgacctacct gtgcgatgcc gcgcgtatgg tgaccttcca tggaccgatg cttaacatcg
    12541 acgtgcgtcc tgacggcgtg gatgaagagt catgggcggc ggcaatgacg tcgggcgaag
    12601 cgtatcagat ggaattcggc aacgacgaag tacaaacgct cgttccgggc agcgcggaag
    12661 gcatgctgta tggaggttgc ctgtcgttgc tatgcgcgtc gctgggcacg ccctacgaga
    12721 tcgagacgcg cggcaccatt ctttttctcg aggacctggc ggagccggcg ttccgcatcg
    12781 atcgcatgct gatgcacttg aagctggcgg gaaaatttga cggcgtccgt ggagtgatct
    12841 tcggagagat gctgaattgc ggtccacgtg acccgcaaga ttacagcctt caacaggttg
    12901 tccagcgcgt gttgggtgac cttggtgtcc ccatggctta cggtctgaaa tctggccacg
    12961 tgagccgagg caacatcacg ctgccgttgg gcgtcgcgac ccagttcacc gctggcgcga
    13021 acgtgtcttt gcggtttgag gctgccgtga aacgtgagtt cgctaaggtt ccagtctcca
    13081 gaccatgaaa cagaagcaca ttcatctaat cgggatatgc ggtaccgcga tggcttcgct
    13141 ggccgggatg ctgaaacagc gtggtcacca cgttaccggt tccgatgttg ccgcatatcc
    13201 gccgatgtca gattttctcg cttcgctggg cattccggtc tcgcagccat tcagcgagaa
    13261 gaaccttgag ccgcgtcccg atctggtgat tgttggtaat gccatttccc gcggtaacgt
    13321 cgaactggaa tacgccctcg accaacggct ggcataccag tccatggcgc acgtcgtgca
    13381 cgaagagttc ctggttgacc gcgagcgttt agtagtagcg ggcacgcacg gcaaaactac
    13441 cacgtcctcc ctgttggcgt ggcagtttac ggtagccctg cgtgcccctg gtttccttat
    13501 cggcggaatt ccggaaaact tcggtagttc gttcgccctc ggaaacggcc ccgagttcat
    13561 tattgaaggc gacgagtacg acaccgcatt cttcgacaag ggcccgaaat tcctgcacta
    13621 tttcccggac gcggtgattc tgacctcggt tgagtttgac cacgcggaca tctacaagga
    13681 tctcgatgaa gtgaaaaccg cgtttaagcg gttggtaaac ctggtgccgc gtcgaggtct
    13741 gctcatcgca tgggacgggc atcccaatgt ggacgagtgc gtgagccgtg ccctttgccg
    13801 ggtcgaacgc tatggctttg gcgagggatc ggcttggcgg atttcagaag caaattacgg
    13861 cgacagtctc acgcgttgga aagtcgcgcg agacggcgct ccctgggcgg agttcgagtc
    13921 gcaactggcg ggcgagtaca acatcctgaa tgctaccgcc gcagctgcga tggcagccaa
    13981 ctatggcatt ccggtggaag tgatagcgaa agcgttgcgg agctttcgca gcgtcaaacg
    14041 gcggctcgaa gtgaaggcgg aaattcacgg aatcaccatc atcgacgact tcgcgcacca
    14101 ccccactgcc atcgccggaa cgctgaaagc actgcgcgag cgataccgcg gacggcgctt
    14161 atgggttgtt cttgaaccgc gttccaacac tctgcgccgc aaagtatttg aaaatgaatt
    14221 ggcggggagc cttgcctttg ccgaccaggt cgtgctggct ggtgtgttca aatcggatgc
    14281 gattccgcag gcagaacggc ttgatcccca gaaggtcatc accaaactga acctcgccgg
    14341 aacgccagca cgcctgctgg catcggccga tgccatcgtg gacgcaattg cattggagtt
    14401 gtgcgaaggc gatgtggttg ccatcctctc caacggcgga ttcggaggca tctatgagaa
    14461 actgcctgcc aagctgaaat cgctgcacga ggcttcgacg ccggcgtgat gcacccgggc
    14521 aaaaccgcac ttgccatctt gcgtctgggc ttcttcgtcg cggtcgcaag cgccgctcag
    14581 ctgtggggag caaccccggt cacgtgttcg tctcctgtgg actcacgacc gcaagcaact
    14641 gagtactttg tttcgctggc cgacaacgcg catcacctgg cgcatgtcag cattcgattt
    14701 ccccagacca gcggcgcaat tgcgctcaag atgccggtgt gggacgctct ctatcaggtg
    14761 cgcgactttg cggcgaacgt gcaaaaggtt cgcgcagctg acgtttcggg gagaccggtt
    14821 tccgttgtca acagcaagac cagcgagtgg aaggttaccg ctccgggccc ctgtgtagtg
    14881 gttgactacg atattcgact cgacaacgac ggtccgtttg gcgcccaact caacgctgaa
    14941 cacggattct tcaattgggc catggtactg atgtactccc ccgccacccg atcgcaaagc
    15001 atgagcttgc agttgctgga cgtccccgcc gcgtggggcc tgcgagacgt tcacatattg
    15061 ggtggcgcgc ccgccgggca agtggagcag gcagtcggag ttgccaccgg ctacgacgaa
    15121 ttggtggaca gtcccgccga ggttggcagc ttccagcagt tcgaatttca gaaggacgga
    15181 gtgacctacc acattgtggt acatggcgat ccccgcgatt atgacggcgg caagctgcag
    15241 gacatcgtcc gcaagatcac ccacgctgca gtggagtgga tggcggatcg gccgttcgac
    15301 gactacacct ttctctatca ctttccgcga agccccggcc atggcggcat ggagcatgct
    15361 tacggaacgg cgattgacat caacgccgaa cgattgcaca acagcatgac gccggttgcc
    15421 agcgtcagcg cccatgaatt cttccacctc tggaacgtca aacgcatccg gcctcagtcg
    15481 ctcgatccga tcgattatca aagggcgatg gacactcgcg cactgtggtt tagcgaaggt
    15541 gtaaccagca cggtcggcga catgctgctg gcgcgagcgg gcctgttgag cgaccggcaa
    15601 tacattgacc gggtcgccag cgagatcaac gaactgcagg tacgcccggc gcaccgctgg
    15661 cagtcggtag aggagtcgag cctcgatgcc tggttcgaag gcgatgcatt ctaccgttcg
    15721 cccgagcgca gcatcagcta ctacaacaaa ggcgaggtgc tcggggtttt gctcgacctg
    15781 cggatgcgtg aacttaccca gggaggaaag tcactgcgcg atctcttcca ttggatgaac
    15841 gagaattacg cgaagaagaa caaacccttc cccgattccg atggagtgca gcaggcggcg
    15901 gaaaccataa ccggacagag tttcgccgag ttctttcaca attatgtggc cggagttctg
    15961 gagttgccct ataacgaata tttccagttc gtgggactcc aggtggcgga aacaacggtg
    16021 cgcacttcga ctcctgggtt tacgaccagc acgaatcctg gcggccagcc ggaagtggct
    16081 tcggtggacg ccaatagcga tgctcaacgg gctggcgttg caatcggcga ccatgtcgtc
    16141 gaggtggatg gaaggcccgc caccgggtca ctcgacgacc aactttcacg aatgcgtgcg
    16201 ggagtgacgg tgaagctcaa cttctctaac cgacggggaa agcgcaccat caaactcaag
    16261 ctcacgtcgc gccaggaaca gatgtacatt ctccaggatg tccccccggt cacgcccgaa
    16321 caactcgcgc atcgcgtggc ctggatacac ggtgacgacg aaagtgctgg tggacagtga
    16381 tccgcacatc attcatgctg acgttctggg gactctcgct cttgatcacg ggaccgttct
    16441 tgctgctcta cacattcctt tccggtaaca tcatgccgct gtatcgcata gcgaacgtcc
    16501 tggcaatcac cggggttcga atggccggcg tgaagattga ggttcgcgga cttgagaact
    16561 taaagtctgg gtgcaattac atttttatgt cgaaccacgt ttcgaacttg gacccgccgg
    16621 ttctgttacc gtcaattccc tgccgatgtt ccgtactggt aaagaaagag gtcttccgca
    16681 ttccgattct cggcaccgcg atgaagatgg cggagttggt cccggtcgat cgcagcaatc
    16741 gcgaggccgc agtggagagc gtccgcgccg gggcggatgt aatgcggcac ggcctcagca
    16801 tgctgatctt tcccgagggg acgcgctcgc ccgatggacg cctcctgccc ttcaagaaag
    16861 gtccgtttca tctggccatg gaatcgagcg tgccagtcat cccggtgacc ctgctcggga
    16921 catcctattg ctggccgaaa agcacatggg cgatgcgtca gggaaacgtg acgctgattt
    16981 ttcatccgcc gatcgacccc tcaggattta cggatcgcga ggatttgatg gcagcggttc
    17041 gccaatccat cgccagcgcg cttcccgaag atcggcggga ctgacaggcg acggacggtc
    17101 aatccaagtg agtcagttcg cgaaacacac tgaagcgctg atcgatttcc tctcgcgtca
    17161 gcgattgcaa tcgctccgtt ccaaactgct ccactgaaaa cgatcccatt acgctgccgt
    17221 aaaacatcgc gtgcttaagg acattgcgag tgacctcgcc ttgcgacgcg atgtatccca
    17281 taaagccgcc ggcaaagcta tcgccagctc ctgtgggatc gcgcacttca tccagaggga
    17341 gcgcgggggc acggaaggga tgatggccac cggcaaattc tccgtcgcgg aagaagatgg
    17401 tcgcgccata ttcgccatgc ttgatgacca gcgccttcgg ccctagtgcg agcaccttgc
    17461 gtgcggcgcg cggcaggctg ctgtcgcccg ccagcatctt agcctcggtg tcgttgatca
    17521 gcaggacgtc aacgcgcttg agcatctcgc cgaggttctg gcgatggtcc ttgatccagt
    17581 aattcatggt gtcgccgccc accagcttca cattgggcat ggcgtcgcgg acctgcgcct
    17641 gcagcaccgg atcaatgttg gcgagaaaca ggaaatccga attttgatat tccgccggaa
    17701 tctgcggctg gaaacttgca aacacgttca ggtcggtctg cagtgtctgc gcctcattca
    17761 cgttgtcgcc gtagtcgcct gcccagaaaa atgttttgcc atcggcgtgc tgaatgccgg
    17821 gcgtatccac gccgcgcttg ttcatcacgg cttcgtgctc ggcggtgaaa tccttgccca
    17881 ccacg
//
LOCUS       DASRKK010000458         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_721093, whole genome shotgun sequence.
ACCESSION   DASRKK010000458 DASRKK010000000
VERSION     DASRKK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721093"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1047
                     /locus_tag="VFC15_10785"
     CDS             409..1047
                     /locus_tag="VFC15_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10684.1"
                     /translation="MRYTALHLIMNNQHGFTPAELRKLGSLKDPYGIQRFLDDMPYHL
                     TDTAWSPRQVLRENTAHCFEGAIFAAAALRANGYPPLIIDFEAEHDTDHVLAIYRVKG
                     HWGAVAKSNYTGCRYREPVYRSLRELAMSYFNIYFNLRRERTLRTFSRPVNLARFDDQ
                     AWMTTEEPIWFIIYYLLEIHHYKLLTPGMVKRLHRIDERLFQGETLGKAYKS"
     gene            1192..2244
                     /locus_tag="VFC15_10790"
     CDS             1192..2244
                     /locus_tag="VFC15_10790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZM10685.1"
                     /translation="MDPETDHLPSDHATLTPTEIAPATQLAALRKIFIGKDGLRAGWS
                     LLVYIVLVAAILFCATAIGHKLHPPAAKSAKTAADMSDPTRAIIGGSIPFLVVVLVTW
                     IMSKIERRRNSVYGLGGSRKLSHFLAGLAWGVTCLSLLVVTLLKTGLLVIDSRLLSGS
                     AILRYGAIWLLGFLLLGLYEEYFTRGYLQYTLTRGLAGFYQWAFKTRHSLALGFWTSA
                     VIFSVLFGLGHKGNPGESPIGLLSAGLAAMVFCFSLWRTGSLWWAIGFHASWDWAQSF
                     LYGVADSGTMVQHHLLATHPVGKPILSGGATGPEGSIFIIAIMALISVIIFFTLPRAH
                     HGYAADGFTQLRPREA"
BASE COUNT          444 a          766 c          597 g          488 t
ORIGIN      
        1 aacaactggc gatggtctgg cagtagttgg acagcagccc gttcccaagg actagacctt
       61 ggccaaattg gcagtttcag gaggcgcaat ctagacagca tccatcaggg gaaccgattg
      121 tcgaggggcc aacctgcttt gcgcgaaggc aggagaggat gattatgtcc cgcattttca
      181 gcagcgccga gccggatcct cagctaactt ggacagcaat gatgggcttc attgacctca
      241 gcggctgaag ccggtttccc gagcgatcgc caaatgcagc gctgaatgaa gcgctgctcc
      301 accctgtttt tccaaatttt tccgcagcct ctcgaagaag cttcaatcgt agattgcgtc
      361 tggccggccc ctgtaacgag gctgtacgca agacgcgagc gaggggcgat gcggtacact
      421 gctctccact taatcatgaa caaccaacac ggcttcactc cggcggaact gcgcaagctg
      481 ggcagtttga aagacccata tggcatacag cgattcctgg acgacatgcc gtaccacctg
      541 accgacaccg cgtggtcgcc tcggcaagtg ttgcgcgaaa acactgccca ttgcttcgaa
      601 ggggcaatct tcgctgcggc tgccctgcgg gccaacggct atccgccgct gattatcgac
      661 tttgaagctg aacacgacac cgaccacgta cttgccattt accgggtgaa gggacattgg
      721 ggcgcggtgg ccaagtcgaa ctacaccggc tgccgctatc gcgagccggt gtaccgttcc
      781 ttgcgcgagc tggccatgag ctacttcaat atctatttca acttgcggcg cgagcgcacg
      841 ctccggacct tttcgcgtcc cgtcaatctt gcgcggttcg acgatcaggc atggatgacc
      901 acggaggagc cgatctggtt catcatttac tacctgctgg aaattcatca ctacaagctg
      961 ctgacgccgg gcatggtcaa gcgcctgcat cgcatcgacg agcgattgtt tcaaggcgag
     1021 acgctgggca aggcgtacaa atcctaaggc aactctgaac gatctgcccg ctgtccgcga
     1081 tggtcaacag gaaaccgatg ccacgctttt tctatgcgca ccgggaacca ccgaaccctc
     1141 ccgtgcgtat aatccttgtg gcatgaattc ttttcacctt aaggaagctc cttggatcct
     1201 gagaccgacc acctgccctc cgatcacgca acgctgacac ccaccgaaat agccccggcc
     1261 acgcagttgg cggctctccg taagattttc attggcaaag acggcctccg cgccggctgg
     1321 agcctgctcg tctacatcgt tctcgtcgcc gccattctct tctgtgcaac cgccatcggc
     1381 cacaagctcc acccgcctgc tgcaaagtcc gcgaagaccg ctgcggacat gtccgatccc
     1441 acgcgcgcca tcatcggcgg gtccattccc ttcctcgtcg tagtcctcgt cacctggatc
     1501 atgtcgaaga tcgagcgacg ccgcaattcc gtctacggat tgggcggcag ccgcaagctc
     1561 tcgcacttcc tcgccggcct tgcctgggga gtcacctgtc tttccctgct cgtcgtgact
     1621 ctcttgaaga ccggactcct cgtcatcgac agccgcctgc tctcgggcag cgccattctc
     1681 cgttacggag cgatctggct gctcggcttc ctcctgttgg gcctatacga ggagtacttc
     1741 acgcgcggct acctccagta cacccttacc cgcggcctcg ccggtttcta ccagtgggcc
     1801 ttcaagaccc gacacagcct agccttgggc ttctggacct cggccgtgat cttctccgtc
     1861 ctcttcggcc tcggccacaa gggcaatccc ggcgagtccc ccattgggct gctctctgcc
     1921 ggcctcgctg ctatggtctt ctgcttcagc ctctggcgca ccggctcgct ctggtgggcc
     1981 atcggcttcc acgcctcatg ggactgggcg caatccttcc tttacggcgt cgccgacagc
     2041 ggaactatgg tccagcacca tctcctcgcc acccaccccg taggcaagcc cattctcagt
     2101 ggcggagcca ccggcccgga aggcagcatc tttatcatcg ccatcatggc cctcatcagc
     2161 gtaatcattt tcttcacctt gccccgcgct catcatggct acgcggcgga tggtttcact
     2221 caactccgtc cacgcgaagc ctaaacattg gcactcccgc ggccaccttg ccaattcgaa
     2281 ccaaaacgga cagaa
//
LOCUS       DASRKK010000459         6771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_525391, whole genome shotgun sequence.
ACCESSION   DASRKK010000459 DASRKK010000000
VERSION     DASRKK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525391"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..515)
                     /locus_tag="VFC15_10795"
     CDS             complement(279..515)
                     /locus_tag="VFC15_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10686.1"
                     /translation="MSSQLKPDLNLCARDLRAIVQKENRIKQALAFRLGLARGLTINA
                     LSAVAGCSQSHVTRMLLLVELPMGDAIAIANGAA"
     gene            618..1685
                     /locus_tag="VFC15_10800"
     CDS             618..1685
                     /locus_tag="VFC15_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM10687.1"
                     /translation="MPKTAPVPECSREDRQVLEEWAHSRTIEARLVERARIVLGCLEG
                     QPVSTVARSWKVRPNTVIDWRRRFAAEGIAGLRDRPRSGKPPRYTGEFRKQVLATLEE
                     PPPKGQAVWDGPALAEYLKTSVHAVWRVLRKEGVCLARQRSWCVSTDPEFTAKGADVV
                     GLYLNPPQNALVLSVDEKPSIQALERKTGFVETDSGKIVRGFKSTYKRHGTLNLFAAL
                     EVATGAVHTQTTGKKRRIEFLEFMDRVVADLPVGQEIHAILDNYCIHKKNDAWLAAHP
                     NVFFHFTPTSASWLNQVEVWFGILSRKALRGASFRDIEQLRKAIEDFVAAYGPKAKPF
                     VWRKREVKGSQLRNTIVNLRN"
     gene            1909..4506
                     /locus_tag="VFC15_10805"
     CDS             1909..4506
                     /locus_tag="VFC15_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM10688.1"
                     /translation="MALTPGTILGQYEIRSQLGAGGMGEVYRAHDTRLDREVAIKVLP
                     ESLTSDLDRLRRFEQEARAAAALNHPNILAVHQMATHEGMSYMVTELLDGETLRERLR
                     RGPIPLRKAIDYGVQIANGLAAAHESGIVHRDLKPENLFLNKDGRAKILDFGLAKLVP
                     APVASPDGRTVTLQEETEPGVVVGTVGYMSPEQVCGGTADQRSDIFALGTILYEMVTG
                     KRAFRKPTSPETMVAILNEDPPPISQLAPDAPAGVQRLVQRCLEKNPEERFYSAHDLA
                     FALEALSDWGISPVPSGPAQESTRPNRMRIATAGVSLLLVLGAAVLGYLWMRPAPVPK
                     VSNYVQLTHDGRHKWLAGTDGSRLYLSYGAYASRSIAEISISGGNLNRIPTPSPNMEV
                     ADISPDGAELLVVDAQGNPDKGPLWSLPVLGGSPRRLGDIVGIDGTWSPDGKMLAYCN
                     GNELSLAKADGTEPRKLVTMKDSDLIYHASWSPDGRHLRFDVQNPADGSRFLWEIAVD
                     GTDLHRLHPGWNKPPDSECCGRWTADGKYFVFVSGGQIWALPQKGGLLHSGPQPVQLT
                     SSPLWLTSPLPSKDGKKLFVVGRTFSGELTRFDVKSGQFAPFLEGISSEYVAFSRDGQ
                     WVAYVSYPGGTLWRSKADGSERMQLTYPPNYAMLPRWSPDGKEIVFSENIADKPSRIY
                     EVSPEGGIPRQLMPDDPQPQRDPNWSPDGSKIVFGGAPYNAASAVRIFDLASRKVSTL
                     PGSQGLFQPRWSPDGRYIAAVSSDSKTLLLFDFQTQKWLEVATGTLDWLNWSKNGEYM
                     QILDSSGAGAVLKIRLSDHKTERVVDLKNFSYTGHLWTGLAIAPDDSPLLLRDVGDQG
                     VYALDWEAP"
     gene            5066..5455
                     /locus_tag="VFC15_10810"
     CDS             5066..5455
                     /locus_tag="VFC15_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10689.1"
                     /translation="MAFDVKELMIDITKACEQPIGCHPSKIVITPTLGCGYTCLPTMF
                     VCHPSVVTTTLLVCKLGTYTCFAGSILTCGGTIYCAGTEEPTVLYQGIDQETLKAVKA
                     QLHRALEEVERHEERSAETAKKPAKRK"
     gene            5600..>6771
                     /locus_tag="VFC15_10815"
     CDS             5600..>6771
                     /locus_tag="VFC15_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10690.1"
                     /translation="MKKGWFVNVLESIFAANPKYELVLFDRLPAEQREMLKDLLKDPN
                     FYGVLRPLEKSGLGVKSVSRDTALLYLTLQQPGRLPAYVQATLGDRYNQEITRLVLDG
                     VLALEREGKFLSGPEAAELICADKKEPAATSAVEGFLERLSLDALRYGQSLGLADPMV
                     LSSRLYGYNREPLSPFWSRILADADSVADYLGIREGSLRVLLDREWSRVPNPAANDGW
                     LAWQSRRVGTRAAFGDRTKIQCKLYVSPKTDHMPQVFPLILAALNRTWVRHVKVGKDA
                     PGLLRPDKMVAYFDSYNDLIDASELLRKELLGCPAHGVPFTAELDGEGLLSWGVDPTA
                     DEHALPWIGRESWRLWVTNRLAVALIAASMDNTSNLEPWQFALRRLQFEGVDTHSWT"
BASE COUNT         1420 a         1878 c         2021 g         1452 t
ORIGIN      
        1 aattaggtca gcagttcggc cgcgttttga tttgccatca gcatcgagtt tcagaatgcg
       61 cgtgcagaag gtcggatgga atcggtgaaa ccacttatct ccggcacggc gaccggaaga
      121 accgattcgc cggcaatcac ggatcgagac tcgaaagtca acggaagatc aggaggaagg
      181 cgtaacgacc ggttgcctcc cagggtgaaa ccagccaaac ccgactacgc ggacgtgcgg
      241 gacttcctga tggatcgcgc cagggcgagg taagggccct acgcagctcc attcgcgata
      301 gcgatggcgt cgcccatagg tagctcaacg agcagcaaca tacgagttac gtgactctgg
      361 ctacagccgg ccacggcgga cagcgcgttg atcgtcaggc cgcgcgccag gccgagtcgg
      421 aacgccaatg cttgcttaat ccggttttct ttctgaacga ttgcgcgcag atctcgcgcg
      481 cacaggttga ggtcaggttt gagttgggaa ctcatgaggt gctccagcgg cgaagatatc
      541 gcccttacac ccctatctag cgcttatttg cattagtgat ggatattacg taatgttgat
      601 tggcaaaggg ggtgcctttg ccaaaaacag cgccggtacc ggaatgttcg cgagaagatc
      661 gccaggtttt agaagaatgg gcgcacagcc gcacgataga agcgcgattg gtggaacgag
      721 cgagaatcgt tttgggatgt ctggaagggc agccggtcag cacggtggcc cggagttgga
      781 aggtgcggcc gaacacggtg atcgattggc gtcggcgctt tgcggcggag ggaatagcgg
      841 gattgcggga ccgtccgcga tcgggcaagc cgccgcgata tacaggggag tttcgcaagc
      901 aagtgctggc cacactggaa gaaccgccgc cgaagggaca agcggtatgg gatgggccgg
      961 cactggcgga gtatttgaaa acatcggtgc atgcggtctg gcgcgtgttg cggaaagaag
     1021 gcgtatgttt ggcacgccag cgcagttggt gcgtcagcac ggacccggag ttcacggcca
     1081 agggcgccga tgtcgtgggt ttgtacttga atcccccaca gaatgcgttg gttctttccg
     1141 tggatgaaaa gccgagtatt caggcgttgg agcgaaagac gggattcgtg gaaacggaca
     1201 gtgggaaaat cgtccgcggt ttcaagagca cctataagcg ccacggcacc ttaaatctgt
     1261 ttgccgccct ggaagtcgcc actggcgccg ttcatacgca gacgaccggc aagaagcggc
     1321 ggattgagtt tctcgaattc atggatcgcg tggtcgccga tctcccggtg ggacaagaaa
     1381 tccacgcgat cttggacaat tactgcatcc acaagaaaaa cgatgcctgg ctggcggctc
     1441 atcccaacgt gttctttcac ttcacgccaa cctcggccag ttggttgaat caagttgaag
     1501 tctggtttgg tattctgagc cgcaaagcac tgcggggagc gtcttttcgc gacatcgagc
     1561 aattgcgcaa agcgattgaa gacttcgtgg cagcctacgg acccaaagcc aagccgttcg
     1621 tctggcgtaa acgcgaagtc aaaggttcgc agttgcgcaa tactatcgtt aacttacgca
     1681 attaagtact ggcatcgctg cagcccacaa caagacttgc caatcttcgg gaaggcggcc
     1741 tcgccgatcg gcggctgttc aggattactt tcggcttggc gatgtcacga cacgttgtct
     1801 gtgcagcccc acttcacaat aactcccgac taatcaacaa ctcatccgtt atagcccatt
     1861 taagcgtaga ggcccggtga ctcgggggta taatccaggc tacctcacat ggcgctgact
     1921 cccggaacga tcctcgggca gtatgaaatt cgttcgcaac tcggcgcggg cggcatgggc
     1981 gaagtgtatc gcgcccacga cacccggctg gaccgcgaag tcgcgatcaa ggtcctaccc
     2041 gaatcgctga catccgatct cgatcgctta cgccgcttcg agcaggaagc gcgtgccgca
     2101 gcggcgctga atcatcccaa tattcttgcc gttcaccaga tggcgacgca cgaaggcatg
     2161 tcgtatatgg tgacggaact gctggacggc gagacgctgc gcgagcgcct ccggcgcggg
     2221 ccgataccgc tgcgcaaggc aatcgactac ggcgtgcaga tcgcgaacgg tttggcggcg
     2281 gcgcacgaaa gcggcatcgt ccatcgcgac ctcaaaccgg agaacctgtt cctaaacaag
     2341 gatggacggg ccaagatcct cgatttcggc cttgccaagc tcgtccccgc tccagttgcg
     2401 tctcccgatg gtcgaaccgt aacgctccaa gaggaaactg aacctggagt ggtggtggga
     2461 accgtgggct acatgtcgcc tgagcaggtg tgcggaggga ctgccgacca gcgctccgac
     2521 atctttgcgt taggcacaat tctctacgag atggtcacag ggaaacgggc atttcgcaag
     2581 ccaacttcgc cggaaaccat ggtcgcaatt ctgaacgaag acccgccgcc gatctcgcag
     2641 cttgcccccg acgctcctgc gggggtgcag agactcgtgc aacgctgcct cgagaagaat
     2701 cctgaggaga ggttttactc ggcacacgat ttggctttcg cgctggaagc gctatcggat
     2761 tggggcatct cgccagtccc ttccggtcct gcgcaggaga gcacgaggcc caatcgcatg
     2821 cggattgcaa ccgcgggagt gtcgctgctg cttgtgctcg gcgctgcggt attaggttac
     2881 ttgtggatgc ggcccgcacc agtgcctaag gtgtccaatt acgtccagct tacgcatgac
     2941 ggccggcata aatggttggc cggaacggac ggttcgaggc tttacctgag ttatggtgca
     3001 tacgcttcac gcagtattgc cgaaatttcg atctcgggag gaaatctcaa caggattccg
     3061 acgccttctc ccaatatgga ggttgctgac atctcaccgg acggggcgga acttctcgtg
     3121 gtggatgcgc agggcaatcc tgacaagggg ccgctctgga gtctgcctgt tctgggtggc
     3181 tccccgcgcc gattaggcga cattgtagga attgacggca cttggtcgcc cgacggcaag
     3241 atgctggctt actgcaacgg gaacgaattg tccctggcga aggcggatgg caccgaaccc
     3301 cgcaagttgg tcacgatgaa ggactctgac cttatctatc atgcctcctg gtcgcccgac
     3361 gggaggcatt tgcgattcga cgttcagaac cctgcagacg gctcacgctt tctttgggag
     3421 attgcggtgg acggtacaga cttgcatcgc ttgcatcccg gttggaacaa gccgcccgac
     3481 tcggaatgct gcggcaggtg gacggccgac ggaaagtact tcgtcttcgt gtcgggcgga
     3541 caaatttggg ccctgccgca aaagggtggt ttgcttcact ccggacccca gcccgttcaa
     3601 ctgacttcca gtcccctgtg gttgacctcg ccgcttccca gcaaagacgg caagaagctc
     3661 ttcgtggtcg gccggacgtt ctcgggcgag ttgacgcgct tcgacgtgaa gtcgggccag
     3721 ttcgcgccct ttctcgaggg gatttcatcc gaatacgtcg ccttctcgcg ggatggccaa
     3781 tgggtggcat acgtttccta tcccggaggc accctgtggc gcagcaaagc ggacgggagc
     3841 gagcgaatgc aactcaccta tccgccgaac tatgccatgc tgccccgctg gtcgccggac
     3901 ggcaaggaga tcgtgttctc tgaaaacatt gctgacaagc cttccagaat ctatgaggtt
     3961 tcacccgagg gaggaattcc ccggcagcta atgccggatg atccacagcc gcaaagggac
     4021 ccgaactggt cgccggacgg gagcaagatc gtcttcggtg gcgcgcccta taatgctgca
     4081 tcggctgttc gaatcttcga tttggcgagc cgcaaagtct ccacgctgcc cggatcacag
     4141 ggtctcttcc agccccggtg gtctcctgac ggaagataca ttgccgccgt atcgtctgac
     4201 tcgaagaccc ttttgttgtt cgattttcag acccagaaat ggcttgaggt ggccacgggc
     4261 acgttggatt ggctgaactg gtcgaagaac ggagagtaca tgcagattct cgatagcagc
     4321 ggagcgggtg cagttctcaa gattcggctc agcgaccata agacggaacg cgtagtagac
     4381 ctgaaaaatt tctcctatac cggccactta tggaccgggc tggcgatcgc gcccgacgat
     4441 tcgccattgc tgctccgtga cgtcggagac cagggcgtct atgccctcga ctgggaagca
     4501 ccataaacgg cgcggttcgc cttgcgggaa tgggtttcac tcatggcacc cattcccgct
     4561 gctgccatcc gtggcgcttc ttgtagtgct tccgatctac cggttttcgc gcaggttgtt
     4621 tcatggtgtg cgtgaattcc cagactacgc aggaccgttc actggcgatt gccgtggctg
     4681 ttgtattgcc ttcctacatt ccgtcgaaaa tcaccacaat tacatctgga ttgccgtgtt
     4741 ggctgtaact ggccctacgg gggttattgc gaccttgtga ccgagcttgt gaaggtctcg
     4801 cgcaacaatg cagccgcggt gcttgcataa ctcgggattg gattctcggc tgtttcgtgt
     4861 ctcgcttttt aatcttgtac aactcgccgc ttcttccagg cgcactcagg tcggtgcata
     4921 attcaggatg aattcggaat tgacatcggt cgacattcgg cctccgacgg ccctgcaaaa
     4981 tcttttgcat gcagaggcaa tcggcgctat agttgcaccg ttcggcaagg ctgagtcttg
     5041 tctaacggca aaggaggtaa aatgtatggc ctttgacgtg aaagaactga tgatcgacat
     5101 taccaaggct tgcgagcagc cgataggctg ccaccccagc aaaattgtaa ttaccccgac
     5161 tttgggctgc gggtacacct gtcttcctac gatgttcgtc tgccacccca gtgtggtaac
     5221 gacaacgttg ttggtgtgca agctcggcac ctacacatgt tttgccggat cgattctgac
     5281 ctgcggtgga acgatttatt gtgctggtac agaagaaccg accgtcctat accagggcat
     5341 tgaccaggaa accctaaagg cggtaaaggc ccagttgcac cgagcgctgg aagaagtgga
     5401 gcggcacgaa gagcgttcag ccgagactgc taagaagccg gcaaagcgaa aatagagggt
     5461 tgcaacagtg gcctccgcac cgtgacggcg ttgcggcatg gctgatggcg cgagtttgtg
     5521 tacctcgctg agcggaagcg cttgttcagg tactctccgc tcagtgtttt gctgttcagc
     5581 ctattcaaga ctatcttcca tgaagaaggg ttggttcgtt aacgtcctgg aatcgatttt
     5641 cgctgcgaat cccaagtacg aattagtact gttcgaccgc ctgcccgccg agcagcgtga
     5701 gatgctgaaa gatctcctga aggatcccaa tttctacggt gttcttcgcc cgcttgagaa
     5761 gtccggcctt ggcgtgaaat cagtttcgcg agacaccgcc ttgctttacc tgacactcca
     5821 gcaaccgggt cggctccccg cgtacgtgca agcgaccctc ggagaccggt acaaccaaga
     5881 gatcacgcgg cttgttctgg atggggtgct ggcgctcgaa agggagggca agtttctctc
     5941 cgggcccgag gccgccgagc tgatttgcgc cgacaagaag gaaccggccg ccacctccgc
     6001 ggtcgaagga ttcctggagc gcctttcatt ggacgctctt cgctacggcc agagtttagg
     6061 cctggctgat ccaatggtgc tttcgtcccg tctctacggg tacaatcgtg agccactgtc
     6121 ccctttttgg agccgaattc ttgccgatgc tgacagcgtc gccgattatc tgggtattcg
     6181 ggagggcagc ctccgggtat tgcttgaccg cgagtggtcg agggtaccca atcccgccgc
     6241 taacgacggc tggcttgcct ggcagtcacg ccgtgttggc acacgggccg ctttcggcga
     6301 tcgtaccaag attcaatgca agctatatgt aagtccgaaa accgaccaca tgcctcaggt
     6361 ctttcccttg attcttgctg cgctgaaccg aacttgggta cgccacgtca aagtcggtaa
     6421 ggatgcgccc ggattactgc gtccggacaa gatggtagcc tacttcgaca gctacaatga
     6481 cttgatagat gcctcagaac tgctgcgaaa ggaactgctg ggctgtccgg cacacggcgt
     6541 cccgtttacg gcggaactgg acggtgaagg cttgctttcg tggggtgtcg atccgacagc
     6601 agacgagcat gcgctgccct ggataggccg agaaagctgg cggctgtggg tgactaaccg
     6661 tctcgctgta gccttgatcg cagccagtat ggataacacc tcaaatctcg agccgtggca
     6721 attcgccctg cgacgactcc agttcgaggg tgtcgataca cactcctgga c
//
LOCUS       DASRKK010000460         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_622471, whole genome shotgun sequence.
ACCESSION   DASRKK010000460 DASRKK010000000
VERSION     DASRKK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622471"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFC15_10820"
     CDS             complement(<1..573)
                     /locus_tag="VFC15_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10691.1"
                     /translation="MLQKDFLSIRDFSPLEIRHFLDVACQLKAYPGAYTGALKGKTLA
                     MIFEKPSLRTRVTFDVGIQQLGGHPLYLSPAEISLGKRESVYDVAKNLERMVQGIMVR
                     TFAHDIVVDMAHHASIPVINGLTDYSHPCQAMADYLTMIEVKGRIAGLKVAYIGDGNN
                     VAHSLLFAGAQLGAEVWVATPPGYEPDSQAV"
     gene            complement(575..1918)
                     /locus_tag="VFC15_10825"
     CDS             complement(575..1918)
                     /locus_tag="VFC15_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HZM10692.1"
                     /translation="MKRVLILGAAGRDFHNFNVVFRNNPEFHVVAFTATQIPDIAGRR
                     YPVQLAGDFYPDGIPILEESKMEDIIHDELVDVVVFSYSDVAYPTLMHLASRAVAAGA
                     DFWLLGVERTQLVAKVPVISICAVRTGCGKSPVSRRIATELRKLGWHPVVVRHPMPYG
                     DLAAQRAQRFGSLADLDKNHCTIEEREEYEPHIMEGTTVYAGVDYEEILRQAEKEGDV
                     VLWDGGNNDTSFYRPDLDIVVLDPHRPGHELAYYPGEVNFRSAHVLIINKVDTADPDG
                     LKTVRQNIALFNPKAQVIETACKVTVADPEAVKGKRVLVVEDGPTLTHGEMPYGAGVV
                     AANRYNAAALVDPRPYAVGSIQKTFEKFPHLWRVLPAMGYSDIQRHELEETIKRVPCD
                     LVLVATPIDLARTIKLDKPSVRVSYEVEEIGKPAITTMLEQFTQAHKPALAEAGR"
     gene            2263..2553
                     /locus_tag="VFC15_10830"
     CDS             2263..2553
                     /locus_tag="VFC15_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZM10693.1"
                     /translation="MAKGSVQIVVERCKACGFCVEFCPTHVLALSSAFNAKGYHTPYM
                     VHPEKCSGCDLCGMYCPDFAIFGHKQPAPPKENAEAAAPPPAAEAKEDQDAR"
BASE COUNT          527 a          763 c          793 g          533 t
ORIGIN      
        1 gacggcctgg ctgtcaggct cgtatcccgg tggcgtggcc acccacacct ccgcgcctag
       61 ttgcgcgccc gcaaatagca gcgagtgcgc cacgttgttg ccgtcgccga tgtaggccac
      121 cttcagtccg gcaattcggc ccttgacctc gatcatggtc aggtagtccg ccattgcctg
      181 gcaggggtgg ctgtaatcgg tgaggccgtt gattaccggg atgctggcgt ggtgggccat
      241 gtccacgacg atgtcgtggg caaaggtccg aaccatgatc ccctgcacca tacgctccag
      301 gttcttggca acatcgtaga ccgactcccg cttgccgaga ctgatctcgg ccggtgaaag
      361 atacagcggg tgcccgccca gttgctggat gcccacgtcg aatgtcactc gcgtgcgcag
      421 cgatggtttt tcgaagatca tggccagcgt cttgcccttc agagcgccgg tatatgcgcc
      481 cggataggcc ttgagctggc aggcgacatc aagaaaatgc ctgatctcca gcggcgagaa
      541 gtcgcgaatc gagaggaaat ctttctgcaa catgctacct cccggcttcc gccaacgccg
      601 gcttgtgcgc ctgggtgaac tgctccagca tcgtggtgat tgcaggcttg ccgatctcct
      661 caacttcgta gctcacccgc acgctgggtt tatccagctt gatggtgcgc gccaggtcga
      721 tcggcgtcgc caccaacact aaatcgcagg ggacgcgctt gatcgtctcc tcgagttcgt
      781 gccgctggat atcgctgtaa cccatggcgg gcaagactct ccaaagatgg ggaaacttct
      841 cgaaggtctt ctggatggag cccacggcat agggacgagg atcgaccaac gcagccgcat
      901 tgtagcgatt ggctgcgacc acgccggctc cgtaaggcat ctctccgtgc gtgagcgtcg
      961 ggccgtcttc gaccacgagc acccgcttgc ccttaactgc ttcgggatcg gcaacggtca
     1021 ccttgcaggc ggtctcgatg acttgcgcct tggggttaaa caacgcgatg ttctgccgca
     1081 ctgtcttaag cccgtcggga tcggcggtgt caaccttgtt gatgatgagc acatgggcgc
     1141 tgcggaagtt gacttcgccg gggtagtagg ccaactcgtg tcccggccga tgcggatcga
     1201 ggacgacgat atcgagatcg ggacgataga acgaagtgtc gttgttaccg ccgtcccaca
     1261 ggacaacatc accctccttc tccgcctgcc gcaggatttc ctcgtaatcc actccagcat
     1321 agacggtggt gccttccatg atgtgaggtt cgtattcctc acgctcttcg atagtgcagt
     1381 gatttttgtc gaggtcggcc agcgaaccga agcgttgcgc acgctgcgcg gcaagatcgc
     1441 catagggcat aggatgacgg accacaacgg ggtgccagcc gagcttgcga agctccgtgg
     1501 cgatacggcg cgagaccggg ctcttgccgc atccggtgcg gacggcgcag atcgagatga
     1561 ccggcacttt cgcaaccaat tgcgtgcgtt cgacgcccag cagccagaaa tcggctccag
     1621 cggcgacggc gcgcgaggcc aggtgcatca gcgtcggata ggcgacgtcg ctgtaggaaa
     1681 agaccaccac gtccacgagt tcgtcgtgga tgatgtcttc cattttgctt tcttcgagga
     1741 tgggaatgcc atccgggtag aagtcgcccg ccaattgcac gggataacgg cggccggcga
     1801 tgtcgggaat ttgtgttgcc gtgaaggcca caacgtggaa ttcaggattg tttcggaaaa
     1861 ctacgttgaa gttgtggaag tctcgtccag cggctcccag gataagcact cttttcatag
     1921 tgcgtaaaaa gcgtcattag acccatgagg tggggacact tttctcctta cagacatggt
     1981 gaaccaagcg ggaggaagtt gctgtgagac cggtcacata gcagtgagac gaagggtgcc
     2041 gaggcgaggc tgaccgagtg ttccgcctca catgggggtg tgaccgctgt cacttcatct
     2101 tgggcatcaa ctagtgaaaa atcgaaccat cgttcggtgg tgggtgagcc tccaaccgca
     2161 acgtaattcc aggtgcgatg ccaagctcaa taagaaggaa gatgcggccg ccaaggcggc
     2221 cactccctcg gcgcagcgcc aagaagataa gaaagggcag caatggccaa gggttcggtg
     2281 cagatcgtag tggagcgatg taaagcctgc ggattctgcg tggagttttg tcccacgcac
     2341 gtcctggcgc tctcctccgc ttttaacgcc aagggttatc acactcctta catggtccat
     2401 ccagagaaat gcagcggctg cgatttgtgc ggcatgtatt gccccgactt cgccatcttc
     2461 ggccacaagc agccagcgcc gcccaaagaa aacgccgagg ccgcggcccc tccaccggct
     2521 gccgaggcca aggaggacca agatgcacgc tgaagccgcc ggtgtcatga ccggcactca
     2581 ttttcttgac ggcgaccact cttgctgcga aggcgc
//
LOCUS       DASRKK010000461         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_36782, whole genome shotgun sequence.
ACCESSION   DASRKK010000461 DASRKK010000000
VERSION     DASRKK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36782"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..836
                     /locus_tag="VFC15_10835"
     CDS             399..836
                     /locus_tag="VFC15_10835"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HZM10694.1"
                     /translation="MTALLGMLALAGYQNRDKISEMLRGATASGSAPGTASQSSPLGG
                     LLGNLGGMLGGAGAGGLLSGGLGELLTKFQANGHGDTARSWIDNGPNQEISPPDLKQA
                     IGPDVLAALEQKTGLSQEELLARLSRELPNAVDKYTPDGRLPH"
     gene            870..1130
                     /locus_tag="VFC15_10840"
     CDS             870..1130
                     /locus_tag="VFC15_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZM10695.1"
                     /translation="MGILWTIIIGFVAGVIAKFIMPGDNEPSGFILTAVLGIIGAFVA
                     TYLGQALGWYGPGQGAGLIGAVVGAVIVLLVYGMVAGRSRAI"
     gene            1157..1591
                     /locus_tag="VFC15_10845"
     CDS             1157..1591
                     /locus_tag="VFC15_10845"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HZM10696.1"
                     /translation="MGIFGKIMGAIFGSHAEAATPSGGTSSTATGGAPPTSSTSGTAK
                     ATPMGAGGASPGPGTVDVAAILDKAVAGKGEKLAWKTSIVDLMKALDIDSSLTARKDL
                     AKELGYTGDTSDSATMNIWLHKKMMEELAANGGKLPADIKKS"
     gene            1940..3190
                     /locus_tag="VFC15_10850"
     CDS             1940..3190
                     /locus_tag="VFC15_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZM10697.1"
                     /translation="MSDTNTDAWVSSSHRPMGFPEFVVMIASIMALNPLAMDMMLPAL
                     PNIASAFHITIANEPQAVLSTFLIGFGVGQFIMGPLSDHFGRRPVLIDGMALYCIASV
                     LAITASSFETLLLARVLQGLGTAATRVIATSIVRDCYAGRRMASVMSLAMMVFIAVPV
                     IAPSLGQAVMLLTQWRGIFIVLMLYGMLAMLWSGLRMPETLPVSERRSLAIRDVLGAF
                     RQTVTNRQTLGYALAGGGVLGVLFAFVFSSQQVFTEIYALGHYFPLAFAAIAIGTAVA
                     GFLNARFVGRVGMRIISHGALVGFVAVAGIMLLAAKVQMLPLPLFVTLAALMMFAFGL
                     MFANFTSLAMEPQGHIAGTASSLYGSITTLLGIGIGTTIGQGYDGTLVPFATGFFLCT
                     LASLAVVLVVEKGRLFKPATRPVT"
     gene            complement(3457..>3770)
                     /locus_tag="VFC15_10855"
     CDS             complement(3457..>3770)
                     /locus_tag="VFC15_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10698.1"
                     /translation="LGVDAMDLKNVLGDIQTDRGNLHLDGSPHVMRLQRSLYGTSMPG
                     AGAVHHIKSGKPQTEHMFSALPPGADICCERQLCARSGRSAKRVEYAWFSSSTVSPSC
                     R"
BASE COUNT          747 a         1056 c         1145 g          822 t
ORIGIN      
        1 gcaaggctgc caagcctctg ctcgacaaag ccggcgtcgc acccgatgac ggtgtgactg
       61 gtctcggcaa ggatttcatc gaagctgcgc ggatgagatt ctgggatcgc gagccttctc
      121 tgcgcaagct cgcctgaacc ataaggcgca ctacaaggcg ggctattatt ttgggccgcc
      181 gacgctaaag ttgaatgatg ctccggcgcg ctgtgcatgg aagtggttgg cgcgtcgcgg
      241 ggggagcagt cgccctgaag acgcggcgtg gattttgcgc atcgtgtcca cggtaccata
      301 tgctcagaaa agggaacaaa atcgccggac ggagcttgga atgtcctgag gaacacgaaa
      361 gaacgcaagg atttgcaatg gccaacggaa tgccttccat gaccgcgctt ctgggcatgc
      421 ttgctctggc cggctaccaa aatcgcgaca agatttcaga gatgctgcga ggcgctacgg
      481 cgtcgggaag cgctcccggg actgcatccc aatcgtcgcc cctcggtggg ttgcttggaa
      541 atctgggggg catgctgggc ggcgccggcg cgggcggtct tctcagtggc ggacttggcg
      601 agttgctcac gaagtttcag gcaaacggcc atggggacac cgctcgctcg tggatcgaca
      661 acggcccaaa tcaggaaatc tcgccgcccg atctcaaaca ggccatcggc cccgatgtgt
      721 tggcggcttt agagcaaaag acaggacttt cgcaagagga gctgttggcg cgtctttccc
      781 gagaattgcc aaacgccgtg gacaagtaca cgcccgacgg ccgacttccc cactaacaga
      841 aatttactca caaacggagg agacacgaga tgggcattct ttggacgatc atcattggtt
      901 tcgtcgcagg tgtaatcgcg aagttcatca tgccgggcga caatgagccg tccggcttca
      961 ttttgaccgc tgtgctcggc atcatcggtg cgttcgttgc gacttatctc gggcaagccc
     1021 ttggctggta tggaccggga caaggcgcgg gtctgatcgg cgccgtcgtc ggcgctgtga
     1081 tcgttctcct tgtttacgga atggtcgcgg gccgaagcag agcgatttaa cactcttcaa
     1141 tcaaaggatt tttcacatgg gtatcttcgg aaaaattatg ggcgcgatct tcggctcgca
     1201 cgcggaagcg gccacgccct ctggcggcac aagcagcact gcaactggtg gagcaccccc
     1261 gacgtcttcg acgagtggca ctgcgaaggc cactcccatg ggcgcgggcg gcgcttcgcc
     1321 gggtccgggg accgtggacg tggcggcgat cctcgataag gccgtcgcag gaaaaggcga
     1381 aaagctggct tggaaaacgt cgatcgtcga cctcatgaag gcgctcgata tcgactccag
     1441 cctaacggct cgcaaggacc tcgcgaaaga actgggatat accggcgaca cgtcggactc
     1501 ggcgacgatg aacatctggc tccacaaaaa aatgatggaa gaactggctg ccaatggcgg
     1561 caagttgcca gcagacatca agaagtcctg aaggttggcg gtgggctcgt gtcttgaggg
     1621 acgcgagcct caaggtagtt aactcgagcg ccggaccatg ccgttcgcgg gaccctcggg
     1681 gcctcgacaa tcgagacgca gaaacttacc gcccgtgaga acaacgatgg tcgagtgtaa
     1741 gaccgttcta ccgtcgtccc ttgctaaagc catttgaatg cagtccgttt gttctggccg
     1801 caaggcttag agtgcgacgc gccggattga cgtccgctaa aatggcctag ggcccgagca
     1861 gcgcaccagc catcccggaa aatgggttgt tcgacgcagc acagcctata actcttcttg
     1921 ttcccgcgag gtgcgctgcg tgagtgatac gaataccgac gcttgggttt catcgagcca
     1981 ccggccgatg ggctttccag aatttgttgt catgatcgct tcgattatgg cgctgaaccc
     2041 gctcgccatg gatatgatgc tgccggcgct accgaacatc gcctcggcct tccacatcac
     2101 tattgccaac gagcctcagg cggttctgtc gacgtttctg atcggtttcg gggtcggcca
     2161 gttcatcatg ggaccacttt cggaccattt cggccgacgt ccggtcctga tcgacggcat
     2221 ggcgctatat tgcatcgcca gcgtgcttgc gattacggcg tcgtctttcg agacattgct
     2281 gctggcgcga gtgctgcagg ggcttggtac cgccgccacc cgtgtgatcg cgacttcgat
     2341 tgttcgcgac tgctacgccg gccgccgcat ggccagcgtc atgtcgctgg cgatgatggt
     2401 cttcatcgca gtgccagtaa ttgcgccgtc attaggtcag gcggtgatgt tactgacgca
     2461 gtggcgtggc atcttcatcg tgttgatgct gtacggaatg cttgcgatgc tctggagcgg
     2521 gctgcggatg ccggaaaccc tcccagtatc cgagcgccgg tcattagcaa tccgcgacgt
     2581 gctcggtgca tttcgccaga ccgtaactaa tcggcagacg ctgggttatg cgttggcggg
     2641 cggcggcgtg ctgggtgtac tcttcgcttt cgtgttctcg tcgcagcagg tgtttaccga
     2701 aatctatgcg ctcgggcatt actttccgtt ggcattcgca gccattgcca tcggcaccgc
     2761 cgtcgctggc tttctcaacg cccgtttcgt tggccgggtc gggatgcgga ttatttcgca
     2821 tggcgcactg gtcggctttg tggcggtggc gggtatcatg cttctggcag cgaaagtgca
     2881 aatgctaccg ctaccattgt tcgttacatt ggcagcgctg atgatgttcg cattcgggct
     2941 gatgttcgcc aatttcacat cgctcgcgat ggagccgcag ggccatatcg ccggcaccgc
     3001 ttcgtcgttg tacgggtcga tcactacgct cctcggaatt ggtatcggca ccacaatcgg
     3061 ccaaggttat gacggcacgc ttgtgccgtt tgcgaccggg tttttcctct gcacgctggc
     3121 gtctttggca gtcgtgttgg tggtagaaaa gggtcggctg ttcaagccgg ccactaggcc
     3181 agttacgtga gccgatggta gaaccttggc accaccctca gtcgtcgcat caccgggtta
     3241 cagtcatgac ccagaaaact catcacgctg ccgaccaagt cgtcgcgtgg cgataaatcc
     3301 gagcgttggc gaggagggcg ttctatcttt gctgaactct ccagcaacag ctggctgccg
     3361 aaccagacga gtttaaccgg atcgtttcaa gcttcattgc acaggtcgat tgtgggcgtt
     3421 ggcccattcg cgatccccgc gcaatcaaga agtcgatcac cggcatgaag gactgacggt
     3481 tgacgaacta aaccatgcat actctacgcg cttggctgaa cggccgctcc tggcgcaaag
     3541 ctgccgttcg cagcaaatgt ccgctcctgg gggcagggcg gaaaacatat gctcagtttg
     3601 aggttttccg cttttgatgt ggtggacggc gcccgctccc ggcatcgaag tgccatagag
     3661 tgatcgttgc agacgcatca catgagggga accgtccaaa tgcaaattac cacgatcggt
     3721 ttggatatcg ccaagaacgt ttttcaaatc catggcatcg acgccaagga
//
LOCUS       DASRKK010000462         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_916063, whole genome shotgun sequence.
ACCESSION   DASRKK010000462 DASRKK010000000
VERSION     DASRKK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916063"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1128)
                     /locus_tag="VFC15_10860"
     CDS             complement(379..1128)
                     /locus_tag="VFC15_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10699.1"
                     /translation="MATVKEIPANLPKHLPGRKYDRQFFFAITLLLVVVVAIGFGPTY
                     YLAGVFRAPLPSRIVHIHGAVFSTWMILLVVQTGLISTRRVEWHRKLGVAGFVLACGM
                     VLTVLLTAADFAMRAKAKPNSEALLTLLVVPFTDAAAFAVLAGLAYALRKNAAAHKRL
                     IIIATAGLTRAALVRWHVPILFQHQHAAYTATYVFLVLLATYDLWSTRKIHRATIWGG
                     VFLILMGQIGGFIGPTAPWHAFAHWVQSLGV"
     gene            complement(1496..2467)
                     /locus_tag="VFC15_10865"
     CDS             complement(1496..2467)
                     /locus_tag="VFC15_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM10700.1"
                     /translation="MLGISHGSAGGVSILKRRDMRKDIEFKTTDGTTLRGWHYLPDGS
                     GKHATIVMAHGFSAVKEMYLDKFADAFAKAGFASVVYDNRNFGASDGQPRQEIDPWLQ
                     VRDYSDAITFALSLDQTDAARIGVWGSSYSGGHVLVVAAIDRRVKCVVSQVPFISGSQ
                     GTRRLVRADFLAGLFAQCAEDRRDRAAGKPPAMIPVVDENPLAVSALPTADSYKWFSE
                     THKSRAPAWRNEVTLKTIEYGFGYEPGAYIGNISPTPLLMIVGVTDHLTVADLAIKAY
                     EQAYEPKKLVLLPGGHFDAYVEEFDQACQPACEWFAAHLLPKDRRRQ"
     gene            2906..>3310
                     /locus_tag="VFC15_10870"
     CDS             2906..>3310
                     /locus_tag="VFC15_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM10701.1"
                     /translation="MTLGELLHSRALENPAKTVLFCEDRTMSLEELDKSTSRLARWFL
                     DEGLQPGDRVAIHWSNRIETVQLFFGLWKAGLIAVPVNIRLKAPEISYILEHSDARMC
                     FSEPTLAQLAAEAGAGSVLSGLPRLEAGDATLP"
BASE COUNT          754 a          962 c          929 g          665 t
ORIGIN      
        1 tggcatgcag atgacgcctg tcgaacgaca ggcttggaaa tcgctcaagc gattgccata
       61 tccacaaacc aactgctgcg gctgactgat ctgatttcta aagttctagc cagttttgct
      121 gacgaaccat gagatggcac aacggtcgat cccggcgctc tcgaaacctg tacccatcaa
      181 aagccttcaa aggctgtgta actaaatagg acgagggcgc agcgcattcc atctaggcca
      241 ggaggtcatc ctccaatcaa aggccgtata catgtgcgca gaccattccg ccaacctcat
      301 tcttcgcttg caaaacggcg gcggaccata catgacgagt gggctaccat caggccggaa
      361 tcccatccag aactgatgtc acacgcccaa actctgaacc caatgtgcga aggcatgcca
      421 aggtgcagtt ggaccgatga agccgccgat ctgtcccata agaataagaa atacgccgcc
      481 ccaaatcgta gcgcgatgaa tcttgcgcgt agaccacaga tcgtatgtgg ccagtaaaac
      541 tagaaagacg tatgtcgcgg tataggctgc gtgctgatgt tgaaacagaa tcggaacgtg
      601 ccagcgaacc agcgccgccc tggtcagtcc ggcagtggca atgataatca gtcgcttgtg
      661 tgccgctgca ttcttgcgca gcgcgtaggc aagccccgca agcacggcga aagcggcagc
      721 gtcggtaaac ggcacaacga gaagagtaag tagcgcctcg gagttgggtt tggccttcgc
      781 ccgcatcgca aaatccgcgg cagtaagaag cacggttagg accattccgc aagccaacac
      841 aaaccccgcc acgccgagct tgcggtgcca ctcaacccgc ctggttgaaa tcaggcccgt
      901 ctgcactaca agaagaatca tccaagtact gaagaccgct ccatgaatgt gcacaatccg
      961 cgacggcagc ggagctcgaa aaacacccgc caggtagtag gtcggcccaa acccaatcgc
     1021 gaccaccacc acaagcagca aggtaatcgc aaagaaaaac tgccggtcat acttacgtcc
     1081 cggcaggtgc ttcggcagat ttgcaggaat ctctttgact gtagccacgt cttcctctca
     1141 atgtgctggt tcagcgtgaa atggagaact ggcgttcctc gttagcgagt ataccgccgt
     1201 gttcggcgga ccagttggga aaacatcgtg cgacgcaagt ggcgactggt gaactgggcg
     1261 gcgggtgaaa ctgcagctcc ccagtctgga cacgtttcga acttttgcga tccaatcggc
     1321 agtgtgatcg aacccatgga cgattttgtt tttcatcatc attgacgcgg agggtcaagc
     1381 tgtatccacg ctggggaccc agtctggaca cgtctcgaac tcgccaaggg aacccggaat
     1441 tagttccgga tgcgcggtca gccaggcgct aaccgtttcc tgatcggcct cgctcctatt
     1501 ggcgacgccg gtccttcggc aagaggtggg cagcgaacca ttcgcaagct ggctgacacg
     1561 cctgatcgaa ctcctccaca taggcgtcga aatgcccacc cggcaacaag acgagcttct
     1621 tgggttcgta ggcctgctcg tacgccttga tggccaggtc cgcgacagtg aggtggtctg
     1681 tgactcccac aatcatcagc aagggcgtcg ggctgatatt gccgatataa gcgccgggtt
     1741 cgtagccgaa gccgtattcg atcgttttga gcgtgacttc gttccgccaa gccggggcgc
     1801 gcgacttgtg cgtttccgaa aaccatttgt acgagtctgc agtcggcaac gccgacaccg
     1861 cgagcggatt ctcgtcaacc accggaatca tggcaggcgg cttgccagcc gcacgatcgc
     1921 ggcggtcctc ggcgcactgg gcgaacaagc cagcgaggaa atcggcccgc acaagcctgc
     1981 gggtgccttg agatccgctg atgaatggca cctgcgagac gacgcatttc acacgccggt
     2041 cgattgcggc gacgaccagc acatggccgc cgctgtagct cgacccccac accccgatgc
     2101 gcgccgcgtc ggtctgatcg aggctcagcg cgaatgtgat ggcgtccgaa tagtccctga
     2161 cttgcagcca cggatcgatt tcctgtcgcg gctggccgtc gctcgcaccg aagttgcggt
     2221 tgtcgtagac gacagaagcg aagcccgcct tagcgaaggc atcggcgaat ttgtcgagat
     2281 acatttcctt cacggccgag aagccatgcg ccatgacgat agttgcatgc ttgccggagc
     2341 cgtccggcag atagtgccaa ccgcgcagtg tcgtgccgtc cgtggtctta aattcgatat
     2401 ctttacgcat gtcccgcctt ttcaaaatcg atacgccgcc cgccgagccg tgactgatgc
     2461 cgagcactct ttagtagctt gcaggtcagg ttaagatacc acatcccaag ggacccgtga
     2521 atctcgaaac caccatcgtg gaagcggcac ggaggatgac aacagaccag tatgagttct
     2581 tccggtgatg ttccatgacc cagtcaggac tcgaacctaa gaacaaagga cttaaccctt
     2641 atgacctctc tgcaggccaa tgttttcaaa tggttggact ttggctcccc agtctggaca
     2701 cgtttcgaac ttttgcggcc agttgaccct ctgatcgaac cagcagacgt ctattgtttt
     2761 cggagagatt cgagcacacc gcggacgcct gtccagggtg ggcacccagt ctggacacgt
     2821 ctcaaactcg ccagggggaa ccatgtaacg tccgcctatc ccgttacttc ttgctcacga
     2881 gaagcatgcg acctacactt gatccatgac tttaggcgaa ctcctgcact ccagagccct
     2941 ggaaaaccca gccaaaacag ttctgttttg cgaagatcgg acgatgtcgc tggaggaact
     3001 ggacaagtcc acgtccagac tggcccgatg gttcctcgac gagggattgc agcccggcga
     3061 ccgtgtcgcc atccattggt cgaatcgtat cgaaacggtg cagttgtttt ttggcttatg
     3121 gaaggccgga ttgatcgcgg ttccggtgaa tatacgtttg aaggcgccgg agatctcgta
     3181 tattctggag cactcggatg ctcgaatgtg cttcagcgaa ccgacactgg cgcagttggc
     3241 ggcggaagcg ggcgcgggaa gcgtcttgtc cgggttgccg cggctggagg cgggcgatgc
     3301 gactctgccg
//
LOCUS       DASRKK010000463         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_427595, whole genome shotgun sequence.
ACCESSION   DASRKK010000463 DASRKK010000000
VERSION     DASRKK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427595"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(663..968)
                     /gene="nuoK"
                     /locus_tag="VFC15_10875"
     CDS             complement(663..968)
                     /gene="nuoK"
                     /locus_tag="VFC15_10875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZM10702.1"
                     /translation="MAEITTMHYLVVAAALFIIGTIGVLTRRNVVIILMSIELILNAV
                     NLNLVAFSRLWGLHGQVFAIFVITDAAAEAAVGLGIIIAFFRNKETVNVDEVDLLKW"
     gene            complement(970..1488)
                     /locus_tag="VFC15_10880"
     CDS             complement(970..1488)
                     /locus_tag="VFC15_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZM10703.1"
                     /translation="MNPVATPFFFYFLAIMAIVSAIAVITRRNPVHAALSLIVTLLSL
                     AGFYLMLYAPFVAAVQIVLYAGGIMVLFLFVIMLVNLEQNIREYQFNKQWIVATIASL
                     ALGGLLLFVYRTGRGSFPSAFYQSYRGLEGDNTQQIGIWLYGNYMLPFEIASLLLLVA
                     IVGAVVMAKKRI"
     gene            complement(1644..>2042)
                     /locus_tag="VFC15_10885"
     CDS             complement(1644..>2042)
                     /locus_tag="VFC15_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZM10704.1"
                     /translation="TFHYQPPSEAITEQYPLERPVIFERFRGQPRLNSNPDTGESLCI
                     VCNLCALACPEQLIVVKGERNPATKRKEVVSWTYDLSRCMFCGLCEEACSTDALELTQ
                     DYEMALYSREGMVLDQAALERGQEPERYAK"
BASE COUNT          473 a          652 c          529 g          388 t
ORIGIN      
        1 cctgcttcga cagcttgcgt ccaaagaaga acatgcaaac cgcgccgatg gcgggaaaca
       61 gcggaatcaa ccagatgtgg tcaagaaaga acatagcaat tagccgttag caattagcaa
      121 ctagcaatta gcaattagca attagcaaaa cccaaaagcc tgtcatcctg agcggagcgc
      181 ccgcttggtc tttccgaccc gctttcgggt cgggcgggcg cgcagccgaa ggatctgcgg
      241 ttgtgccgtc cccgagggac tcggttcatt tatccacgcc cacccaccgc tgaagcggtg
      301 ggctatcttc ttccgtcccg cttcgcggga ctggggcaat cgtcagtgca aagatcgtca
      361 caacctgcgg aattcatacc gcattcactc gcgaggctaa agccccgatc aatatcgagt
      421 cccgttgacg cggccctaaa gggctgcact acctgaccgc tgcctacgcg gccctgaagg
      481 gccgcactac ccattcgtcg tctcttccgt cccgcttcgc gggactgaat catcgtcact
      541 cgaaccaaat ttccaaagaa cagttaatgg cgcatcggcc aaaaccaatc tcgaagaaca
      601 aatcgccgcc gcatcggcca aaaaaactga cccctagccc gtagccccaa gcccctgctt
      661 ccctaccact tcagcaagtc cacttcgtcc acgttcaccg tttccttgtt acggaagaag
      721 gcgatgatga tgcccagccc gactgccgct tccgcggctg catcggtgat cacgaagatc
      781 gcgaacacct gcccgtgcaa tccccacaac cgcgagaacg ccaccaggtt caggttcacg
      841 gcattcagga tcaactcaat cgacatcagg atgatgacca cgttgcgccg cgtcagcacc
      901 ccgatagtgc caatgatgaa cagcgccgcc gcaaccacga ggtaatgcat tgtggtgatc
      961 tctgccatgt caaatcctct tcttcgccat gaccaccgcg cctacgatgg ccaccagcaa
     1021 caacagcgac gcaatctcga acgggagcat gtaattgccg tacagccaga ttccaatctg
     1081 ctgcgtgttg tcgccctcga gcccgcgata actctggtag aaagccgacg gaaacgagcc
     1141 tcgaccggtg cgatacacga acagcagcaa tccaccgagc gccagcgagg caatcgtcgc
     1201 cacgatccac tgcttgttga actggtactc gcggatgttc tgctccaggt tcaccagcat
     1261 gatgacgaac aggaagagca ccatgattcc gccggcgtac agcacgatct gcacggcagc
     1321 cacgaacggc gcgtacagca tcaggtaaaa accggccagc gagagcaggg tcacgatcag
     1381 cgacagcgcc gcgtgcaccg gattccgccg cgtaatcacc gcgatggcgg aaacgatggc
     1441 catgatcgcg agaaagtaga agaagaacgg tgtcgcgaca ggattcatat tgtgtgtttc
     1501 acattcattg gtgacaacct tgggcttcgt ctttcgccgg ctgaaaacca gatcttcttt
     1561 gcaaatcgtc tttgacgctg gcctaaaggc cagctctacc aaactggcaa ctgcctactg
     1621 actgctggct actgacaact gcctcatttc gcgtacctct ccggttcctg cccgcgctcc
     1681 agcgcggcct ggtccagcac catgccctcg cggctgtaga gcgccatctc gtaatcctgg
     1741 gtcagttcca gcgcatcggt cgagcacgcc tcctcacaca ggccgcagaa catgcagcgg
     1801 ctcaggtcgt aggtccacga gacgacctct ttgcgcttgg tcgcgggatt gcgctcgccc
     1861 ttcaccacga tcaattgttc gggacaggcc agcgcgcaca ggttgcaaac gatgcacagc
     1921 gactcgccgg tatcgggatt tgagttcaag cgcggctgcc cgcggaaacg ctcgaagatc
     1981 accgggcgct ccagcggata ctgctcagta attgcctcac tgggcggttg atagtggaac
     2041 gt
//
LOCUS       DASRKK010000464         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_818430, whole genome shotgun sequence.
ACCESSION   DASRKK010000464 DASRKK010000000
VERSION     DASRKK010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818430"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..410)
                     /gene="flgM"
                     /locus_tag="VFC15_10890"
     CDS             complement(192..410)
                     /gene="flgM"
                     /locus_tag="VFC15_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZM10705.1"
                     /translation="MTHRLSASAEEVAHLSTRSAAVQVLKAQLDKVPETRQQKVDSLK
                     QAIAEGRHRRDPRQIAAAMFADGILSLG"
     gene            complement(1009..1395)
                     /locus_tag="VFC15_10895"
     CDS             complement(1009..1395)
                     /locus_tag="VFC15_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM10706.1"
                     /translation="MPGLRTLIVDDSSLMRKVAVRALQQAGLAIAEVLEAGNGSEALD
                     ILRHQRVDLVLSDMNMPVMDGLEFMRQLGSVENACGVPVLMLTTEDSESQVMEAISCS
                     AAGYIRKPFTPDQMKQHVLPLLAKTV"
     gene            complement(1399..>2302)
                     /locus_tag="VFC15_10900"
     CDS             complement(1399..>2302)
                     /locus_tag="VFC15_10900"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZM10707.1"
                     /translation="AARPATLDHRSSYANGLTLLRGPDCESSGLPLLCLPILTRSASL
                     LNLLLQDGAVDLELTSSVVALDPGLAFTTLQLANRERREEDELIWQFPLAVVAGGQHR
                     LLQAVNQAPKIESYSSTKIRAELRQLWVRAVVRACLARALSQQVGGGNPRQAFLAGLL
                     LELPALVELTFPSCPSSQLGLQAAWRESLPPEIAAAIGQARDCDHVHAPSKDGPQTSL
                     AASIRLAELLLGCTMHESSPRLGKGRELASNSVWQCWQETSLRQHHLLLDRCCELARW
                     AAAHAPGMNPWEFTARLERSKGWE"
BASE COUNT          425 a          741 c          685 g          451 t
ORIGIN      
        1 actaccctgc tcctccgcca gatcttctgc ctccgccagc tcactctcat gcttggaccg
       61 cgcggtgcac gccctttgcg ctctcccgct ggcgtgctga accggcgctc cggggtccgc
      121 gatgctgctt ccaggcggcc aatgctcagc tccattgcca tccatccttc tgcatgggga
      181 agctagtggc ttcagccgag actgagtatt ccatcggcaa acatcgcagc cgcgatctgt
      241 cgcgggtccc ggcggtgcct tccctcagca atggcttgct tcaacgaatc caccttctgc
      301 tgccgggtct ccgggacctt gtccagttgt gccttcagaa cttggaccgc agcgctcctg
      361 gtggacagat gcgcaacctc ttccgcgctt gccgacagcc ggtgcgtcat gtcgcggatc
      421 ttccatcgga agtggggctc ttgctgcggt ccatctccgc catggctgac ggatctaagc
      481 caattctcat cgcagtacct cgtgctatgt atgtccatgc aattaagtaa tcggcaatag
      541 ttaagaaagc tttaatcttt ctgcggtttt ttcccgttgc ttgcggattc ctccacgttc
      601 tgccagcgct gaactgggca gttgcgcctt ggaaccgttg ttatcgacgt tggctaaagc
      661 ggtcccagga ttgctgtatc cgctcgttcg ggtagtgcgg gccttcaggc ccgcgttcaa
      721 gggcgagagg aaaatcgctt cagccgctga ggtcgatgac ctcagcggct gaagccatga
      781 attgcgaggg cgtagctaac gcgggcttga ccaccccaac ccgcgcaaag acggcgcggg
      841 ttgggggccc cggctgaagg cccgcactac ccgagctaca gccactctgg tgctgctcta
      901 gaaagctagg cgacgggaaa ctcacgtctg aatgacggtt gccgctcccg agtgaacgga
      961 cgcggccagt cgccgtgcag catgcaggga gcacctttga accggcggtc ataccgtctt
     1021 cgccagcaag gggagcacat gctgtttcat ctggtcgggc gtgaaaggct tgcggatgta
     1081 gccggcagcg ctgcaagaga tcgcttccat cacctgcgac tcgctgtctt cggtggtgag
     1141 catgagcacc ggcacgccgc acgcgttctc taccgacccc agttggcgca taaactccaa
     1201 accatccatg accggcatgt tcatgtcgct caataccaga tcgaccctct ggtgacggag
     1261 aatatcgagg gcctcggaac cattgcccgc ctccagcacc tctgcaatcg caaggcctgc
     1321 ctgctgcagc gcgcgcaccg cgactttgcg catcaaggaa gaatcatcga cgatcaacgt
     1381 gcgtagccct ggcatggctt actcccagcc tttgcttctt tccagcctgg cggtaaattc
     1441 ccagggattc attcccggcg cgtgggcggc tgcccacctg gccagctcgc aacaacgatc
     1501 cagcagcaga tggtgctggc gcaggctggt ttcctgccaa cattgccaca cggagttcga
     1561 ggccagctct ctgcctttcc cgagccgtgg cgatgactcg tgcatcgtgc atcccaatag
     1621 caactctgcc agccggatgc tcgcggccag cgaagtttgc ggcccgtctt tagacggagc
     1681 gtgaacgtga tcgcagtcgc gtgcttggcc gatggccgcg gcaatctccg gcggcagact
     1741 ttcgcgccac gcggcttgca gcccaagctg ggacgatggg caggacggga aggtcaattc
     1801 gaccagcgcc ggcaactcca acagcagtcc tgccaggaag gcttgccgcg gattcccgcc
     1861 gcccacctgt tggctcaggg cccgcgcaag gcaagcccgg accacggccc gcacccagag
     1921 ctggcgcaac tcggcccgga ttttggtaga ggaataggac tcgatcttcg gcgcctgatt
     1981 gaccgcttgc agcagacggt gctgcccacc cgccactacg gccagcggaa attgccagat
     2041 aagctcatcc tcctcgcggc gttcgcgatt cgccaactgc agcgtggtga aggccagccc
     2101 cggatccagg gccaccaccg agctcgtcaa ctccaggtcg acggccccat cctgcagcag
     2161 aagattgagc agcgaggcag agcgggtcag aatgggcagg cagagaagag gcaggccgga
     2221 cgattcgcaa tcgggaccac gcagtaaggt gaggccattg gcgtacgacg agcggtgatc
     2281 gagtgtggct ggccgggctg cg
//
LOCUS       DASRKK010000465         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_622813, whole genome shotgun sequence.
ACCESSION   DASRKK010000465 DASRKK010000000
VERSION     DASRKK010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622813"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFC15_10905"
     CDS             complement(<1..1004)
                     /locus_tag="VFC15_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZM10708.1"
                     /translation="MTVIKQDDLIQSVADALQYISYYHPVDFIQGLARAYEKEQSPAA
                     RDAIAQLLINSRMCAEGHRPICQDTGIVSVFLKVGMKVQWDATMSVEDMVNEGVRRAY
                     LHPDNKLRASVLADPAGERKNTKDNTPAVVNMTLVPGDKVDVIVAAKGGGSEAKSKFV
                     MLNPSDSIVEWVLKTVPTMGSGWCPPGIIGIGIGGTAEKAMLLAKEALMEPIDIQELM
                     TRGPKNRAEELRVELYKKINELGIGAQGLGGLTTVLDVKVKDYPTHAANLPVAMIPNC
                     AATRHAHFVLDGSGAVALQPPSLKDWPELTYNPAGARRVNLDTVTRDEVATWKTGEVI
                     L"
     gene            1078..2088
                     /locus_tag="VFC15_10910"
     CDS             1078..2088
                     /locus_tag="VFC15_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM10709.1"
                     /translation="MTPREFNVRGVIGIPQTWDIALNFPRSFSVIMPSSSGEWMRLTI
                     DNVSKTYRSGVRALHNFSLELGSGVLGLLGPNGAGKSTLMSILATITQPSAGRVVWED
                     GSSSFDIVQHPNALRQVLGYLPQEFGFYPKLTALEFLEYLAAVKGIPSAAAKRRIQEL
                     LQLVNLVDACKRPLGGFSGGMRQRVGIAQALLNDPKLLIVDEPTAGLDPEERVRFRNL
                     ISELAADRIVILSTHIVSDVESTANLIALLNKGELLRHVSPEDLLAAVEGRVWECVVS
                     SADFPLFKQHHRVSGVVRRADGFHVRVVSEDRPWGNAVLQTAQLEDAYLYCLSARGEV
                     YA"
     gene            2085..3731
                     /locus_tag="VFC15_10915"
     CDS             2085..3731
                     /locus_tag="VFC15_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10710.1"
                     /translation="MNAIASELHIVWQVALADYRERTRRYPFMVAFLFCAWLSSTIYT
                     DKTQVTLGANFGYFNSAWSTAVVAVLANMVLSVMGFFLVKNTVERDEQTRVGPLLAAT
                     PLASRDYLFGKCLSNFLYLMSLGLVFMVSVPFLQHHRGPQYPIEPVKIVSEFLLLVTP
                     LAAFIAALAVLWESVPLLKRGLGNVLYVVVWMAVLLNSAGRESSPTDLFGFVQFMRST
                     HGAEVAQGLKASRDFELNIGHMESDHIGRFLWNGFSWNASTVLWRAEWFLVAIILCLV
                     ALYWFKRFDPDYARSFVESRWQSMGARFRRRRVDSGTAQENKWTAAVLPQMFRDRYSQ
                     VGRYSEQFQFVGILRAELRLMLRGVPKPAYALIALINFIALVTPRSEKMNLLPLLWLL
                     PVLLWSQMGTREQRNNTAALLFSAPHSFLRQLPALWCAGVLVSLVSATGMVMRAILIG
                     DHHLVASCIAGAVFIPSLALACGVWSNTPRLFEALYVGFWYLALNGAPFADYMGLTPQ
                     APAMRFLVMGCCLSAAALLRRWWDVERGPAQRLLAVGNRA"
BASE COUNT          730 a         1185 c         1239 g          916 t
ORIGIN      
        1 agaatcacct cgccagtctt ccacgtcgcc acttcgtcgc gcgtgaccgt gtccaggttc
       61 acgcgtcgcg cacccgccgg attgtaggtc aattccggcc agtccttcag cgacggcggc
      121 tgcaacgcga ccgcgcccga gccgtcgaga acaaagtgag catggcgtgt ggccgcgcaa
      181 ttcgggatca tcgctacggg caggttggcg gcgtgcgtcg gatagtcctt caccttcaca
      241 tcaagcacgg tggtcaaccc gcccaatccc tgcgcaccga tgccaagctc gttgatcttt
      301 ttgtataact cgacccgcaa ctcctcggcg cggttcttcg gcccgcgggt catcagctcc
      361 tggatgtcaa tcggctccat cagggcttcc ttggccagca gcatggcttt ctcggccgtg
      421 ccgccgatgc cgatgccgat aattcccgga ggacaccagc ccgagcccat cgtggggact
      481 gtcttcaaca cccactcgac gatggagtcg gagggattca gcatgacgaa cttcgactta
      541 gcttccgagc cgccaccctt tgcggcgacg atgacgtcaa ccttgtcgcc gggaacaagc
      601 gtcatgttca cgacggcggg ggtattgtcc ttggtgttct tgcgttcgcc ggccggatcg
      661 gccagcacac tggcgcgcag cttgttgtcg gggtggaggt acgcgcggcg cacgccctcg
      721 ttgaccatgt cctccacact catggtcgcg tcccactgca ccttcatacc gaccttcagg
      781 aagacggaca cgatcccggt gtcctgacag atgggccgat ggccttcggc gcacatgcgg
      841 gagttgatca gcaattgagc gatggcatcg cgagcggcgg gcgactgttc tttctcgtac
      901 gcgcgcgcca ggccctgaat gaaatcgacc gggtggtagt aggaaatgta ttgcagagcg
      961 tcggcaacgc tctggataag atcgtcttgc ttgatgactg tcattaccgc ctcgctataa
     1021 gaaagagcgt aagaatccgg acacacgccc acagattgat tataggaagt cagatgcatg
     1081 acacctagag aattcaatgt gcgcggagta atcggcattc cccaaacgtg ggacatagca
     1141 ttgaactttc cgcggtcgtt ttccgtaatc atgcccagca gctcaggaga atggatgcgc
     1201 ctcacaatcg acaacgtaag caaaacctat cgctcgggcg tgcgtgcact gcacaacttc
     1261 tcactcgaac tcggctcggg tgtgcttggt ctcctgggcc cgaacggagc agggaagagc
     1321 acgctaatga gcatcctggc caccatcacc cagcccagcg ctgggcgcgt ggtttgggag
     1381 gatgggagct ccagtttcga tatcgtgcag catcccaatg ccttgcgaca ggttctcggt
     1441 tatctgccgc aggaatttgg gttttacccc aagctgaccg cgctggagtt tcttgagtac
     1501 ctcgcggcgg tcaagggcat tccgtcggcc gcagccaagc ggcgtatcca ggagctattg
     1561 caactggtca acctggtgga cgcgtgcaag cggccattgg gcggcttctc cggcggcatg
     1621 cggcaacgcg tcggaattgc acaggcgttg ctgaacgatc cgaagctgct catcgttgac
     1681 gaaccgaccg cgggcttgga tccggaggag cgcgtccgct tccgcaactt gatctcggaa
     1741 ctcgccgccg accgcatcgt cattctctcc acgcacatcg tgtcggacgt cgagtccacg
     1801 gccaatctaa tcgcgctgct gaacaaaggt gagctgctga ggcatgtctc tcctgaggac
     1861 ctgctggccg ctgtagaagg ccgggtgtgg gagtgcgtgg tgtcgtctgc tgactttccg
     1921 ttattcaagc agcaccatcg ggttagcggt gtggtgcgcc gcgccgacgg atttcacgtt
     1981 cgcgtggtct ccgaagacag gccgtgggga aacgctgtct tgcaaaccgc acagctggag
     2041 gacgcctatc tgtactgcct gagcgcccgg ggcgaggttt acgcatgaac gcgattgcga
     2101 gcgaacttca cattgtttgg caggtggcgc tggccgatta ccgggagcgc acccggcgat
     2161 atccattcat ggtcgcgttc ctgttctgcg cgtggctttc gtccaccatc tacaccgaca
     2221 agacgcaggt aacgctgggg gccaatttcg gctatttcaa ttcggcatgg agtacggctg
     2281 tcgtcgccgt tctcgccaac atggtgctta gtgtgatggg attcttcctg gtgaagaata
     2341 ccgtcgagcg cgacgaacag actcgtgtgg ggccactcct ggctgccaca cccctggcca
     2401 gtcgtgacta tctatttggg aagtgcctca gcaatttctt gtatctcatg tcgctgggat
     2461 tggtcttcat ggtttccgtg ccatttctac agcaccatcg cggacctcaa tatcccatcg
     2521 aaccggtgaa gatcgtcagc gaatttctgc tgctggtgac gccgctggcg gcatttattg
     2581 ccgcattggc ggtgctgtgg gagtcggtcc cgttgctgaa gcgcgggctg ggaaacgtgc
     2641 tgtacgtggt cgtctggatg gccgttctgt tgaactcggc aggccgggag agttcgccta
     2701 ccgacctgtt cggtttcgta caattcatgc gaagcacgca tggggccgaa gtcgcacaag
     2761 ggctcaaggc ttcgcgtgat ttcgaactca atatcggaca catggagtcg gaccacattg
     2821 ggcgattcct gtggaatgga ttttcctgga acgcgagcac ggtgctgtgg cgcgccgaat
     2881 ggtttctcgt cgcgataatc ctatgcctcg tggctctcta ctggttcaag cgcttcgatc
     2941 ccgactacgc tcgatcgttc gtcgagagcc gctggcaatc catgggtgcg cgcttccgcc
     3001 gcaggagagt cgactcgggg accgcgcagg aaaacaagtg gacggctgcg gtgctgccgc
     3061 agatgtttcg cgaccgctat tcgcaagtgg ggcgttactc ggagcagttt cagttcgtgg
     3121 gcatcctacg cgcggagctt cgcctgatgc tgcgcggtgt tcccaagccg gcgtatgcgc
     3181 tgatagccct gattaatttc atcgcgctgg tcacccccag gagcgagaag atgaacctgc
     3241 tgcctctgtt gtggctgctt ccggtcttgc tgtggtcgca aatgggaacg cgcgaacagc
     3301 gtaacaacac ggccgctctt ctgttttcgg ccccacattc cttcctacgg caacttccgg
     3361 cgctgtggtg cgctggagtg ctggtatcgc tggtctccgc gacaggcatg gttatgaggg
     3421 cgatcctaat cggcgatcat catctcgtcg catcctgtat cgccggcgct gtgttcatcc
     3481 cgtcgctggc gttggcgtgc ggagtttggt cgaacacgcc gcgcctcttt gaagcgttgt
     3541 acgttggttt ttggtacctg gcgttgaatg gcgcgccctt cgccgactac atgggcttga
     3601 cgccgcaagc gcctgccatg cgtttcctgg ttatgggatg ttgcctgtcc gcggcagctc
     3661 ttctgcggcg gtggtgggat gtcgagcgcg gccccgcgca gcggttattg gctgtcggga
     3721 atcgcgcttg agttaggcgg cttgctctac gggtcaagtc ctgatcggaa tggctgacga
     3781 gagaagctgc tcatcgtcca gttgctgagc ggactgaaca ctggcggctt atggcagttt
     3841 cacatctggt atatagacga tgggtggagc acgccttcgg gcgtgcataa ctgccgtcat
     3901 ctaggctggc agcttctcac accagcttcc cgtacatcga cgtatagttc aggccaatct
     3961 ccccgcagct gccttgttct accgaataga caatgcagga gtcgaacggg cgtgagtaaa
     4021 cgtgcaggct cacggcgcgc tgtccccatt cgcgcggatt gcggacgtcg
//
LOCUS       DASRKK010000466        10321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1014650, whole genome shotgun sequence.
ACCESSION   DASRKK010000466 DASRKK010000000
VERSION     DASRKK010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014650"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFC15_10920"
     CDS             complement(<1..222)
                     /locus_tag="VFC15_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10711.1"
                     /translation="MADKTEEHLRHNRAPLAYENKKFLNSPEGRILRILAEYQEPLSR
                     FRRERIQDTIVFFGSARFHSRSQAEETLQL"
     gene            complement(432..776)
                     /locus_tag="VFC15_10925"
     CDS             complement(432..776)
                     /locus_tag="VFC15_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10712.1"
                     /translation="MSAFAPERPQEGNGLEPSGPVLLMPPPLPPKKPHLWLQRIWLVV
                     FVLFCLEVGIILTALPWTGIWTENSLLLGYPQVREFLMQNFVRGLVSGIGLVDIWMGV
                     AEAVRYRESPDA"
     gene            997..1605
                     /locus_tag="VFC15_10930"
     CDS             997..1605
                     /locus_tag="VFC15_10930"
                     /inference="COORDINATES: protein motif:HMM:NF037410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10713.1"
                     /translation="MMQQQQGPKLYSCVVQHDNGQAPNPYFGVCTLCLCKFRECPTQR
                     RSIVEKAKKGDWIFGTGGADRGKSAGHGKLIYAMQVDEKVTRWEYFTDDRFKEKKPLK
                     TRNYKQTRGDNKRPRNAFEKHEQFALISRHFYYFGRDAISIPKDFNLEKKFQGYRYNF
                     PPTDMRRFVEWIEKRRKPGRRGEPCYREFDEPKGNNKCKLSC"
     gene            1587..2309
                     /locus_tag="VFC15_10935"
     CDS             1587..2309
                     /locus_tag="VFC15_10935"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10714.1"
                     /translation="MQVVLLRVGIDTGSGGIHGPLFSDGCFEYIPIPDSFRGKGVGQR
                     TYGNTTGRRGPRLVDYFPKTRRETVCNQSIHFDPEFETFTYGDPTRPKASLRQLSEGS
                     LLVFYAGLKGWDYDCPPALYIIGCFEVERAGLASSFSQSELQGMFQSNFHVMHGDVFE
                     DQKDRLVLVRGNANSRLLKKAVKISSVGTDRRGRALQRLSPEMQEVFGGFGGNTSIQR
                     SPPRWVTPEFTDRAAQFVLALR"
     gene            2388..2528
                     /locus_tag="VFC15_10940"
     CDS             2388..2528
                     /locus_tag="VFC15_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10715.1"
                     /translation="MSNPEMRKKLAALSFSEKIKILEKLRRRSLAIAAAGLRPHKTKS
                     AE"
     gene            complement(2598..4073)
                     /locus_tag="VFC15_10945"
     CDS             complement(2598..4073)
                     /locus_tag="VFC15_10945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZM10716.1"
                     /translation="MPTDVIMPQMGESIFEGTITKWLKQPGEKVQRDEPLFEISTDKV
                     DAEIPAPASGTLQEIKVQAGATVQVNTVVGTIAGEGEAAGAAPSAPAAGTAPPAAPSA
                     PAATTAPPATAGGDGSRSTQTEAAQPPSGEEPKKPSASQTGKESAQPAQEKTGVEPAQ
                     QGAVLEYPEAGEQDRVRSSPLVRRIAKDNKVDMAQVPGTGLGGRITKEDIESFIAKHG
                     AGGPQPAAPAQPAAVAAPSAAAPSIPATVAPLQPVGGVTPGEVVPMSVMRKKIAEHMV
                     LSKRTSAHVHGVFEVDVTRIVKLREKMKTNFEQATGNKLTFTPFFARAVVHGIRQWPI
                     INSSVEGENIHYHASINLGIAVALDWGLIVPVVKNAEERSFVGLQRAITDLGERARTK
                     KLKPDEVQGGTFTITNPGIFGAKFGMPIINQPQVAIMGVGGIMKKPVVVTDKDGNDSI
                     AIRSMMHLSIGYDHRIVDGAVADQFMAEVKQFLENWNEELL"
     gene            complement(4165..5175)
                     /locus_tag="VFC15_10950"
     CDS             complement(4165..5175)
                     /locus_tag="VFC15_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZM10717.1"
                     /translation="MKAAVVQKPQSPIVIEDRPMHEPKSGEVLIRVHACGVCHSDLMV
                     QQGLFPFAKYPMVPGHEVAGVVEKVGPGVTWPQVGDRVGMPWLFSSCGHCKLCVQGDE
                     VMCPFAEVTGVTKDGGYQEYMIAPAAYVAPIPDGLDFAEAGPLMCAGLTVFNGLRNAR
                     FQPGQKVAVIGLGGLGHLGVLYAKAMGARVAVISSSPDKEAEARGLGAEQFINTKSKK
                     SGDALKEWDGGADIILATAPSTEAATDAFPGLAPDGTLVVLGVGPGNITASPMDLVGG
                     RRHLMGSPSGSRKDIRATLDFSLHNRVLPHITKFPLEDAGKALDLMHQGKLSGRAVLI
                     LR"
     gene            5698..6909
                     /locus_tag="VFC15_10955"
     CDS             5698..6909
                     /locus_tag="VFC15_10955"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM10718.1"
                     /translation="MRLLIDQAGLRSSLFAVATFSPVQILCFAAFLAAGGAIVYVLFG
                     YPLLLGWIAKRHNHPVKKDDQLRSVSIIIAVRNGEKFIRRKLESVLALNYPRELVEII
                     VVSDGSDDGTDEIAREFEAQRVRLLRVPRGGKAVALNAAVPCASGEILIFTDVRQQVD
                     PQALRNLIANFGDPAIGAVSGELAIVNDQTQEESDTSLYWKYELWLRKQMCRIDSSFG
                     CTGAFYGVRRSLFQPFPPDILLDDAYLPLTIFLKGYRVVYEPTAQMYDLPTALDSEFR
                     RKVRTQAGLYQFLRIMPELLSAKNRMCLHFLSGKFGRMIIPYCLILMAVATIGMSPGW
                     RMAAAIAQLGFYLLALLDYVLPASFPLKHLTSPIRTFVVLMTASLLGVKVLFVSSASL
                     WKETKVRHPAA"
     gene            7006..8823
                     /gene="ggt"
                     /locus_tag="VFC15_10960"
     CDS             7006..8823
                     /gene="ggt"
                     /locus_tag="VFC15_10960"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZM10719.1"
                     /translation="MSGHDSGSPAFIFMQLRRQLLQVVLALALCAQALAGNGMHPVHA
                     PKAMVATLQPEASKAGVAIMRQGGNAVDAAVAVGFALAVVLPQAGNIGGGGFLLFRRA
                     NGEVHFLDFREKAPAKATANMYLDAQGNVVPGMSTLGYKSIGVPGSVAGLVFAQKHWG
                     KLTLKQVMEPAIRLAREGYVLDYDAARSLHDPNLAKFPESHRIFQRDGNFYQTGDVFK
                     QPALAKTLEHIAENPDDFYHGAMARELAAAIEKGGGLITAEDLAQYEVKERQPIRGTY
                     RGYQIISAPPPSSGGVTLVESLNILEGYDLGKQGEGSAQNIHLTAEAYQRAFFDRAEF
                     LGDPDFSKVPVAQLLDKRYANAWRETIPLRRATPGNELRRPSVFSQLDSYASSHPQPK
                     AFREPVHTTHYSVVDPEGNAVSVTTTLNESFGSHVTAEGLGFLLNDEMDDFASKPGAP
                     NMFGLIQGPANAIGPGKRPLSAMAPTIVLKDGKLFLVLGAEGGPTIITTVANVLMGVV
                     DYGLNIQQAVNAPRFHEQWLPDEIKLETVGFSPDTVRILEHMGHKVSADASIGSPPGQ
                     RHGPKQEYWGDSQCIEIDPDTGERHGASDGRNNGKAVGF"
     gene            8835..9647
                     /locus_tag="VFC15_10965"
     CDS             8835..9647
                     /locus_tag="VFC15_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZM10720.1"
                     /translation="MSTLIYARRERLHPGLLDQMVAGGAEAIEIFAARGHFNYTDRQH
                     IRQIANWFRSTGVTFHSMHSPMHPDEDWGRGGATHINIASVEKLQRIAATDEIKRAIE
                     VAEQAPFEFLVQHVGIGHEEADEHKLDAAMSSLEHLHAFAKPLGVQLLVENITNELST
                     PETLLDLLHAGRFDDIGVCFDVGHAHIMSSVPQAFEVLKDRIRSTHVHDNKQERDSHL
                     WPGAGTIDWDETMELLRTAPHVLPVLLEIEGVEGERVSEKMAEAFGKLEATS"
     gene            complement(9690..10007)
                     /locus_tag="VFC15_10970"
     CDS             complement(9690..10007)
                     /locus_tag="VFC15_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10721.1"
                     /translation="MLFDHGTPRGLARLLVGHTIRVARAQGWDTLSNGELLAQAEAAG
                     FDVLVTPDKNIRYQQNLEGRNIAIVVLGNPLWPIVRLHGELVLSAVNAAMPGSYTEVD
                     IPG"
     gene            complement(10015..10248)
                     /locus_tag="VFC15_10975"
     CDS             complement(10015..10248)
                     /locus_tag="VFC15_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZM10722.1"
                     /translation="MPLDWSQCPAVESIPGKVSGAWVLRGTRMPVSIIFENLDSGMTV
                     EEVMEQFDVTREQVHAVLEFVTRSLHRDPMYTP"
BASE COUNT         2140 a         3025 c         3017 g         2139 t
ORIGIN      
        1 cagttgcagg gtttcttccg cttgcgagcg gctatgaaag cgcgccgagc caaagaacac
       61 aatcgtgtcc tggatgcgtt cgcggcgaaa gcgcgacagc ggctcctggt attcggcaag
      121 gatgcgcagg atgcggccct cggggctgtt gagaaatttc ttgttctcgt aggccagcgg
      181 cgcgcgattg tggcgaagat gttcttcggt cttgtcagcc atggaattgc tgcctcagtc
      241 catcgagagt ttccaggaga tgcatgttgg atgcgaacag cgtgtctgca agtgagagcg
      301 gcacgttaag gaaactctga acaagtcgat gttcgggtag cgccggcctt caggctggcg
      361 tctacgctgc ttcaaatatc ccgggggctt cagccccgaa aatacggaat gtcgattttt
      421 tcagggcctc ctcaggcgtc cggggattcg cgatagcgga ccgcctcggc gactcccatc
      481 caaatgtcca ccaggccgat cccgctgacc aagccgcgca cgaaattctg catgaggaat
      541 tcccgcactt gcgggtatcc cagcagaagc gagttctctg tccagatgcc ggtccagggc
      601 agcgcggtga ggatgattcc cacttccaga cagaacaaca cgaataccac cagccaaatc
      661 cgctgtagcc agagatgcgg cttcttcgga ggaagcggag gtggcatcag cagcacggga
      721 ccgctgggct ccagcccatt accttcctgc ggccgctctg gcgcaaaagc gctcatggtc
      781 cgcgcttggt ccagtgatcg tcctccgaat ggcgtcgcct ctaccacaat cgttcctact
      841 aagatgccca atttgttcgt ccggcgttcc aatgctcagg acatgaagtc cacatagcag
      901 attctatcag tgcgcgcgaa aagggatcct tctgaaacgc ggcggggctt cggaaacatc
      961 caagcgcgac ctgaagctgg tgtgttgtag actcggatga tgcaacaaca gcaagggccg
     1021 aagctttact cctgtgttgt tcaacatgat aatgggcaag cgcccaaccc gtattttggc
     1081 gtgtgtacgc tttgcctgtg caaattccgc gaatgtccta cacagcgcag atcaatcgtt
     1141 gaaaaggcaa aaaaaggtga ttggatcttc ggtacgggcg gggctgaccg agggaagagc
     1201 gccggccacg ggaagctcat ttacgctatg caggtcgatg aaaaagttac gcgatgggaa
     1261 tatttcactg acgacagatt caaggagaag aaacccctga agacgcgcaa ctataaacag
     1321 acacggggtg acaacaaacg gcctcgcaat gcttttgaga agcatgagca gtttgctctc
     1381 atctcacgcc acttttacta cttcgggcgg gacgcaatta gtatccccaa agattttaac
     1441 ctcgaaaaaa aattccaggg gtaccgctac aattttcccc caacagatat gcgccggttc
     1501 gttgagtgga tcgagaaacg tcgcaaaccc ggaagacgag gggagccgtg ttacagggaa
     1561 ttcgatgaac caaaaggaaa caataaatgc aagttgtctt gctgagagtc ggtatagaca
     1621 ctggttccgg gggaattcac ggcccgctgt tcagcgatgg ttgttttgag tacatcccaa
     1681 ttccggacag ctttagaggt aagggagttg gccagaggac gtacgggaac accactggca
     1741 gacgaggtcc caggctagtc gactacttcc ctaagactcg ccgagaaacg gtctgcaacc
     1801 aatccatcca cttcgatcct gaatttgaaa cattcacata cggcgacccc acgcgcccca
     1861 aagcctcact acgtcagctc agcgaaggca gcctactggt gttctacgcg ggcctcaagg
     1921 ggtgggacta tgactgcccg ccagctctgt acatcatcgg ctgctttgaa gtagagcgag
     1981 cgggtttggc cagttccttc agccaatcgg aactgcaagg gatgttccaa agtaactttc
     2041 acgtcatgca cggtgatgtt ttcgaagacc agaaggaccg actggttctg gtaagaggca
     2101 acgcgaacag ccggttgctg aaaaaagcgg tcaaaatcag ttcggtggga acggacagac
     2161 gtggccgcgc ccttcagagg ctctcgccag aaatgcagga ggtattcggg ggattcgggg
     2221 gcaacaccag catccagcgg agtccgccga gatgggtaac accggagttc accgaccgcg
     2281 cagctcagtt tgtcttggct cttcggtgag tcgggtttac gttgtcttcc gatggcgccc
     2341 agaattattc cgcacttcaa ccgaatggaa gacactgaag acactgaatg agtaatccag
     2401 aaatgcggaa aaagctcgca gcgctcagtt tttccgagaa gatcaaaatt ctggaaaagc
     2461 tgcggcgccg cagtctggcc attgctgccg ccggtttgcg accgcataaa accaagtccg
     2521 cagagtaaaa cggcagggct cactgaccct gccgttcttt ttcgctgaca actgacaact
     2581 ggctgctgac aactgaccta cagcagctcc tcgttccagt tctccaggaa ctgcttcacc
     2641 tcggccatga actgatcggc cacagctccg tcaacgatgc gatggtcgta gccgatgctg
     2701 aggtgcatca tcgaacggat tgcgatggag tcgttgccgt ccttgtctgt aactaccacc
     2761 ggcttcttca tgattccacc cacgcccatg atggcgacct gcggctgatt gatgatgggc
     2821 atcccgaatt tcgcgccgaa gatgccgggg ttggtgatgg tgaacgtccc accctgcact
     2881 tcatcgggct tcaacttctt ggtgcgggcg cgctcgccga ggtcggtgat cgcccgctgc
     2941 aagcccacga agctgcgctc ctcggcgttc ttcacaacgg gcacgattag gccccaatcc
     3001 agcgcgaccg cgataccaag attgatggaa gcgtgatagt ggatgttctc gccttccacg
     3061 gacgagttga tgatgggcca ttggcgaatg ccgtgcacca cggcgcgcgc aaagaacggc
     3121 gtaaaggtga gtttgttacc ggtggcctgc tcgaagttcg tcttcatctt ctcgcgcaat
     3181 ttcacgatgc gggtcacgtc cacttcgaag acgccgtgca cgtgcgcgct ggtgcgcttc
     3241 gacagcacca tgtgctcggc aattttcttg cgcatgaccg acatgggcac aacctcgccg
     3301 ggagtaacgc cgccgacagg ttgcagaggc gcgacagtcg caggtatcga aggtgcggca
     3361 gcggagggcg cggcaaccgc agccggttgc gccggagcag cgggctgagg cccgccagcg
     3421 ccgtgcttgg cgatgaacga ctcgatgtct tccttggtga tgcggccgcc gaggccagtt
     3481 cccggaacct gcgccatgtc aaccttgttg tccttggcga tgcggcgcac cagcggactg
     3541 gaacgcacgc ggtcctgctc cccagcttcg ggatactcga gcactgcacc ctgctgtgcg
     3601 ggttccacac ccgtcttctc ctgggcgggc tgtgctgact ccttgccggt ctgggacgca
     3661 gaaggcttct tgggctcctc gccactcgga ggctgcgccg cttcggtctg ggtcgagcgg
     3721 ctgccgtctc ctccggcagt ggccggtggt gcggtggtgg cagccggagc ggatggcgcg
     3781 gcaggcggtg cggtgccggc agccggagcg gatggtgcgg ctccggcagc ttcgccttcg
     3841 cccgcgatag tccccaccac ggtgttcacc tggaccgtgg cgccggcttg gactttaatc
     3901 tcctgcaaag tgccggaagc aggcgcggga atctcggcgt caaccttgtc ggtcgagatt
     3961 tcaaacagcg gctcgtcgcg ttgaactttt tcacccggct gcttcagcca cttggtgatg
     4021 gttccttcaa aaatcgattc acccatctgg ggcatgatca cgtctgttgg catgttgtcc
     4081 ttccgcggga tacatagctg gaactgcgag cttgcagcaa acgaacatta tatcgcgtaa
     4141 aacggggcga tcatctacgg cgcttcagcg caatatcagt acggcccgtc cgctcagctt
     4201 gccttgatgc atcagatcaa gcgccttgcc cgcatcttcc agtgggaatt tggtgatatg
     4261 cggcagcacg cgattgtgga gtgagaagtc cagcgtcgca cgaatgtctt ttcggctgcc
     4321 cgaaggcgag cccatcaggt ggcgacgccc gccaaccaga tccatgggag acgccgtgat
     4381 attgcccggt ccaaccccga gcacgaccag cgttccatcc ggagcgaggc cggggaaggc
     4441 atcagttgcg gcttccgtgc tgggcgcagt ggccagaatg atgtcggctc cgccatccca
     4501 ttccttcagc gcatcacccg acttcttcga tttcgtgtta atgaattgct cggcgcctag
     4561 tccgcgagcc tcggcttcct tgtcgggcga actcgatatg acggccacgc gtgcgcccat
     4621 cgctttggcg taaagcacgc cgaggtgtcc gaggccgccg agtccgatga cggctacctt
     4681 ttgcccgggc tgaaatctcg cgttgcgcag tccattgaat acggtcaatc cggcgcacat
     4741 gagcggaccg gcctcggcaa aatccaaccc gtcgggaatc ggcgcgacgt acgccgcggg
     4801 cgcgatcatg tactcctggt agccgccgtc tttggtcacg ccggtgacct cggcgaaggg
     4861 gcacatcacc tcgtcgccct gcacgcacag tttgcagtgg ccgcaggagg agaacagcca
     4921 tggcattccg acgcgatcgc caacctgagg ccaggtgacc cccggcccaa ctttctccac
     4981 cacgccggcc acttcatgtc cgggaaccat gggatacttc gcaaaaggaa agaggccttg
     5041 ctgcaccatg agatcgctgt ggcatacacc gcaggcatga acgcgaatca gcacttcgcc
     5101 ggatttcggc tcatgcatgg gacgatcttc gatgacgatc ggggattgcg gtttctgaac
     5161 gacggctgct ttcatagtct cctgctctca gttgaacaaa tggggtttga ataacgagag
     5221 aatcgcactg aagtttattc gatgagacaa tttaccgaaa cgatacatcg caaagtgagg
     5281 ttcgctgtgg tggtttgact cagtggaacg cgtggattac tctcgacgca gaaaagcctt
     5341 cgctgttagt gcgttctttg aaagggccca gctttagctg tgccgtatac cgttgacaat
     5401 cgaagaccgc cccatacgcc ggcccaaggc cgcagctttt aagcgacagt aactttcatg
     5461 gatgccctct ctctttcgtt aactcgggtg tgatgaaatg tggagtttcg agacctactg
     5521 agtgctgccg aaatacattt gatgatatgg ctcgacggta tgatccagcc agccatggac
     5581 cattcagttt ctgtgaccgg aagttgcggc agcagcctca gctacaatcg attgtgactc
     5641 gcggagctgc accggggaac gaagtccggc acccacgaga tctgcggctg cgccaacatg
     5701 agactattga ttgatcaggc cgggttgagg tcgagcttgt tcgccgtcgc gacgttctca
     5761 cctgtccaga ttctttgctt cgccgcgttt ctggccgccg gaggcgccat cgtctacgtg
     5821 ctgttcggct accccttgct gctgggatgg atagcgaaga ggcacaacca tccggtcaaa
     5881 aaggacgacc agttgcgttc ggtgtccatc atcatcgccg tccgcaatgg ggagaagttc
     5941 attcgccgaa agctggaatc ggtgttggct ctgaattacc cccgcgaact ggtggagatc
     6001 atcgtcgtat ccgacggttc tgacgacggc accgacgaga tcgcacgcga gttcgaggcc
     6061 cagcgagtcc gactgctgcg agttccgcga ggaggcaagg cggtcgcgct caatgcagca
     6121 gtgccttgcg cctcaggcga gatcctcatt ttcaccgatg tccgccagca agtggatcct
     6181 caagcgcttc ggaatctgat cgcgaacttc ggcgacccgg cgatcggcgc cgtcagcggc
     6241 gagcttgcta tcgtaaacga ccagacacag gaagagtccg acactagtct ctactggaag
     6301 tacgaacttt ggctgcgcaa gcagatgtgc cgcatcgact ccagcttcgg ctgtaccggc
     6361 gcgttttatg gagtacggcg ttcgctgttc cagcccttcc cgccggatat cctccttgac
     6421 gatgcgtatt tgccgctgac catctttctt aagggctacc gggtggttta cgagccgacg
     6481 gcgcagatgt atgaccttcc caccgctctg gattcggagt ttcgtcgcaa ggtgcgcacc
     6541 caggccggcc tatatcagtt cctgcgcatc atgcccgagt tgctgagtgc gaagaaccgc
     6601 atgtgtttgc atttcctctc cggcaagttc ggacgcatga tcattccgta ctgcctcatc
     6661 ctcatggccg tggctacaat cggaatgtcg cccggttgga gaatggccgc agcaatcgcc
     6721 cagttagggt tctacctgct tgcactgctc gactatgttt tgcctgcaag tttcccctta
     6781 aagcatctga cttcgcccat ccgcaccttc gtcgtgctga tgaccgcgtc tttgctgggc
     6841 gtaaaggtct tgttcgtgtc gtcggcaagt ttgtggaaag aaaccaaggt tcgccacccc
     6901 gcggcgtgat tccacgctcg ggaacatatt tgactcccct cgtcgcgctg ggctagcctc
     6961 aatgttttgt acttgcatgg ccgtaacttc ttgcgccgaa cgcatttgag cggccatgat
     7021 tcgggttctc ccgccttcat ctttatgcaa ctgcgacgcc aactgctgca ggtggtactt
     7081 gcgctggctc tttgcgccca ggctctcgcc ggcaatggga tgcatccagt gcatgctccc
     7141 aaagccatgg tggccacgct gcaacccgag gcatccaagg cgggcgtcgc gatcatgcgc
     7201 cagggcggca acgcagtgga cgccgcggtt gcggtcggct tcgccctcgc cgtggtgctt
     7261 ccccaggcag gcaacatcgg cggaggaggc ttcctgttat tccgccgggc caacggcgag
     7321 gtccacttcc tcgacttccg cgagaaagcc ccggccaaag ccactgccaa catgtatctg
     7381 gatgctcagg gcaatgtggt tccaggcatg agcacgctcg gctacaaatc gatcggcgtc
     7441 cccggttcgg tggccggcct ggtattcgcg cagaagcatt ggggtaagct cacgctgaag
     7501 caggtaatgg agcctgcaat ccgtctggcg cgcgaaggct acgtgctcga ctacgacgcg
     7561 gcgaggtcgc tccacgatcc caacctggcg aagtttcccg aatcgcatcg catctttcag
     7621 cgggacggca acttctacca aacgggcgac gtcttcaagc agccagcgct ggcgaagact
     7681 ctggagcaca tcgccgaaaa tcctgacgac ttctaccatg gcgcgatggc gcgtgagctg
     7741 gctgcggcca tcgagaaggg cggcggcctc atcaccgccg aggaccttgc ccagtacgag
     7801 gtgaaggagc ggcagccaat tcgtggcacc tatcgagggt accagatcat cagcgctccc
     7861 ccgccgtctt ctggaggggt taccctggtc gaaagcctca acatcctcga aggatacgac
     7921 ctaggtaagc agggtgaagg ctcggcgcag aacattcatc tgaccgccga ggcttatcag
     7981 cgcgcatttt tcgaccgcgc cgagtttctt ggagaccctg atttctccaa ggtcccggtt
     8041 gcgcaactgc tggacaagcg atacgccaat gcctggcgcg aaaccattcc gctgcgccgc
     8101 gccacgcccg gcaatgaact gcggcgtcct tctgtcttct cgcaactgga cagctatgcc
     8161 agttctcatc cgcagccaaa ggcgtttcgc gagccggttc acaccaccca ctattcggta
     8221 gtcgatcctg agggcaacgc ggtatcggtc acaaccacgc tgaacgagag ctttggctcg
     8281 catgtcactg ccgagggctt gggcttcctg ctcaacgacg aaatggacga tttcgcctcc
     8341 aagccaggcg cgcccaatat gttcggactg attcagggcc ccgcgaacgc aataggccca
     8401 ggcaagcggc cgctgtccgc catggcgccg accatcgtgc taaaggacgg caagctcttc
     8461 ctggttctcg gcgctgaagg gggtcccacg atcatcacta cggtcgccaa tgtactgatg
     8521 ggagtggtgg actacggatt gaacattcag caggcggtca acgcgccccg ctttcacgag
     8581 cagtggctgc ccgacgaaat caagctggag acggttggct tctcgcccga cacggttcgc
     8641 atcctcgagc acatgggaca caaagtgtcc gccgacgcga gcatcggctc tcccccggga
     8701 cagcggcatg gcccgaagca ggaatattgg ggcgactccc agtgcatcga gatcgacccc
     8761 gacaccggcg agcgccacgg cgccagtgat ggacgcaata acggaaaggc ggtcggattc
     8821 tgatgcgcag ggcgatgtcc actctgatct atgcgaggcg ggaacggctg catcctggcc
     8881 tgctcgacca gatggtcgcg ggcggggcgg aagccatcga aatcttcgcc gcgcgcgggc
     8941 acttcaacta cactgaccgg cagcacatcc ggcagatcgc caactggttt cgcagcaccg
     9001 gagtgacgtt ccactcgatg cactcgccca tgcatcccga cgaagattgg ggacgcggcg
     9061 gcgcgacgca catcaacatt gcgtcggttg agaagcttca gcgaatcgcg gccacggatg
     9121 aaatcaagcg cgccatcgag gtggccgagc aggcgccatt tgagtttctg gtgcagcatg
     9181 tcggcattgg ccacgaagag gcggacgagc acaaactgga tgcggcaatg agctccctcg
     9241 aacacctgca cgctttcgcc aagcccctgg gcgtgcaact gctggtcgag aacattacca
     9301 acgaactttc gacgccggag acgttgctgg acctgctgca tgccgggcgc ttcgacgata
     9361 tcggcgtgtg cttcgacgtc ggccatgcgc acatcatgtc gagcgtgccg caagcgttcg
     9421 aggtgctcaa agaccgcatc cgctcaacgc acgtgcacga caacaaacag gagcgcgatt
     9481 cgcacctctg gcccggtgcg ggcactattg attgggacga gacgatggag ctattgcgga
     9541 ctgcgccgca cgtgctgccg gtgttactcg agatcgaagg cgtggaaggc gagagggttt
     9601 cggagaagat ggcggaggcg ttcggtaagc tggaagccac gtcgtaaggg ctgccaaaga
     9661 ttactcgaag aaaacctacg acgtgacctt caacccggta tgtccacttc ggtgtagctt
     9721 cccggcatag ccgcatttac cgcggacaga actagttcgc cgtgcagtcg cactatgggc
     9781 cacaacggat ttcctagaac aacgattgca atgtttcggc cttcaaggtt ttgctgatag
     9841 cggatgtttt tatcaggagt aaccagcacg tcgaatccgg ctgcttccgc ttgtgcgagt
     9901 aactccccgt tgcttaaggt gtcccatccc tgggctctcg ctactctgat cgtatggcca
     9961 accagcaagc gcgccagccc tcgcggagtt ccgtggtcga agagcacaag catcttaggg
    10021 ggtgtacatg gggtccctgt gcaggctgcg tgtgacaaac tccagcactg cgtggacctg
    10081 ttcgcgggtg acgtcgaact gttccattac ttcctcgacc gtcataccgg aatcgaggtt
    10141 ctcgaaaata atggatacag gcatgcgcgt tccgcgtagt acccatgccc cactcacctt
    10201 gccgggtatg ctttcgacgg cgggacattg tgaccagtca agcggcatag taagctccta
    10261 gagtcattct aacgctgtcg gaatcaggac tcatggacac atcgacaaaa atggaaatca
    10321 c
//
LOCUS       DASRKK010000467         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1405728, whole genome shotgun sequence.
ACCESSION   DASRKK010000467 DASRKK010000000
VERSION     DASRKK010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405728"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..2194
                     /locus_tag="VFC15_10980"
     CDS             248..2194
                     /locus_tag="VFC15_10980"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10723.1"
                     /translation="MPYSFFRRRTIAALFTAISIALTTAVYAQSPAGPQPVPLPPPIA
                     APADMPYPGTISLSVDITNITDHVLNVHETVPVKGREITLLYPEWLPGTHSPSNPVEK
                     LAGLIVTANGKRVDWVRDRVNMYAFHVSVPQGVTTLEISFQCLAPLDPKHGRISSNFA
                     DVTWNSALLYPAGYFSRRILFDPAIRLPEGWKFATALELGSQDGNLIHFKPTTLNTLV
                     DSPLYAGVNYKRVDLSTGADNPVFLDLFADKPADLEITPEELQYHKNLVIEAQKLFNS
                     HHYDHYDFLFSLSDTVGGEGLEHHQSSEDGTRANYFTDWAAGVGGRALLAHEYAHSWN
                     GKFRRPADLWTPNFNVPMQNDLLWVYEGLTDYYGNVLAARSGMRIAEQARDVIAQIAA
                     YFDISLGRTWRPLLDTTNQPITSSHGADRETWPSWQRSYDYYPESDLIWLDADTKLRE
                     LSNGSKSLDDFARLFFSIDNGSYITRTYTFDDLVAALNKVQPFDWAGFLRARVYDLAT
                     QTPKDGITRGGYRLVYNDDQPEWLKHQDSSRGTSFATSLGFSVDKDNGLENVWWDSLA
                     FKAGITPDMHPEAVNDQKFTVAGLREAILAAEKSKQPIKLLLKRGDDFVTVTLDYYGG
                     LRYPHLERVEGTPALLDAILAPSK"
     gene            complement(2757..3044)
                     /locus_tag="VFC15_10985"
     CDS             complement(2757..3044)
                     /locus_tag="VFC15_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10724.1"
                     /translation="MFFGIRAGISDARSRREPFVFGVLFDSQRRRALLKEAFERLSVL
                     IAMSILLGALSQFLILHEVFPGPALIVGPVLIAMPYSLTRALTNRIVRHSR"
     gene            complement(3857..4168)
                     /locus_tag="VFC15_10990"
     CDS             complement(3857..4168)
                     /locus_tag="VFC15_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM10725.1"
                     /translation="MPAEVSIGQIFLEFLIIGATSFGGVVPYLRGSLWRTSIQQGLAP
                     VTIGLLLAGCFTMAKGAIFGAETAAIAVGVLLILLQYKVNPALLVLGSAVVGVLSFMP
                     K"
     gene            complement(4215..>4703)
                     /locus_tag="VFC15_10995"
     CDS             complement(4215..>4703)
                     /locus_tag="VFC15_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10726.1"
                     /translation="FKAEVLPLKNEVGGYRILYSHNCQFGVSQMHDADKTSMATLVKV
                     FPALAALQKSPDERVSLVNEGIVEEAPLPLGQFDFGKGLVGKCDMGLWRTRGEHKSLV
                     GEFAFQVKFDRKEDVAEKQKKLVAQFYVTLQQEIQDWLALGVTKTGMVYRLKGNEPQS
                     HE"
BASE COUNT         1035 a         1379 c         1307 g          982 t
ORIGIN      
        1 gcaccagtgg gaagtcggcc ttagcttgcc gaacgtgatc cttgtgacaa ggtgccactc
       61 ccggcagctc catacaagtt tctggtacca gtgatcgttc cgaaggcacg atcgaatgct
      121 tttataggac ttctggggaa cctaccgtct atcggaacca tcaataaatt cggttcgtcc
      181 atgaaagatt gcgtgtcatc tgtgtgcgat cggctgtaca gtgttgtctg gattttggag
      241 gatcggcgtg ccatattcgt tcttccgtcg tcgcaccatt gcggcgcttt tcaccgctat
      301 ttccattgct ttgaccaccg cggtgtatgc ccagagcccg gcgggcccgc agcctgtacc
      361 gctgcctccg cccattgccg cgccggcaga catgccttat cccggcacca tctctctgag
      421 cgtcgatatc accaacatca ccgatcatgt gttgaatgtg catgagacgg taccggtaaa
      481 aggccgcgag attacgctgc tctacccaga gtggcttccc gggacgcact ccccatcgaa
      541 cccggtggag aagttggctg ggctcatagt cacggcgaat ggcaagcgcg tcgactgggt
      601 tcgcgatcgc gtcaatatgt acgccttcca cgtctccgta ccgcagggcg taaccaccct
      661 ggaaatcagt tttcagtgtt tggctcccct ggacccaaag catggtcgta tctcgtccaa
      721 cttcgccgac gttacctgga acagcgcgct gctttatccg gcgggctact tctcgcggcg
      781 catcctgttt gatcccgcca tccgcctgcc ggagggctgg aaattcgcca ctgcgctgga
      841 gctgggatcg caggatggaa acctgattca cttcaaaccg actacgctga acactctggt
      901 ggactcgccg ctctatgccg gcgtcaatta caagcgcgtc gacctgtcca ccggcgcgga
      961 caacccggta ttcctcgatc tcttcgccga caagcctgcc gatctcgaaa tcacgccgga
     1021 ggagctgcaa taccacaaga accttgtgat cgaagcgcag aaactcttca actcccatca
     1081 ctacgatcac tatgattttc ttttttcgct cagcgatacg gttggcggcg agggcctgga
     1141 acaccatcag tccagtgagg acggtacccg tgccaactac tttaccgact gggctgcggg
     1201 ggttggcggt cgtgctctcc tcgcccacga gtacgcccat tcgtggaacg gcaagtttcg
     1261 gcggcccgcc gacctgtgga cgcccaactt caacgtgccg atgcagaacg acctgctatg
     1321 ggtttatgaa gggctgaccg attactacgg caacgtcctg gcagctcgtt ccggaatgcg
     1381 tatcgcggag caggcccgcg acgtgatcgc gcagatcgcc gcctacttcg acatcagcct
     1441 tggacgtacc tggcgcccct tgttggatac caccaatcag cccatcactt cgtctcacgg
     1501 cgccgaccgg gagacctggc caagctggca gcgttcctac gactactatc cggaatcaga
     1561 tcttatctgg ctcgatgctg acacgaaact ccgcgaattg agcaacggca gcaagtctct
     1621 cgatgatttc gccaggctct tcttcagcat cgacaacggt agctacatca ccaggactta
     1681 taccttcgac gatctggtcg cggcattgaa taaggtgcag ccatttgatt gggcagggtt
     1741 cctgcgcgcg cgggtctacg acctggcgac gcagactccg aaggatggaa ttacccgagg
     1801 cggttatcgg ctcgtctaca atgacgacca gccggagtgg ctgaaacacc aggacagctc
     1861 ccgcggaacg agctttgcca cgtcactggg gttctcggtg gacaaagaca atggccttga
     1921 aaacgtgtgg tgggatagtc tggcgtttaa ggcaggcatc acgccggata tgcacccgga
     1981 agcagttaat gatcaaaaat tcactgttgc gggcctgcgc gaagccatcc tcgccgcaga
     2041 gaagagtaag cagccgataa aacttctgct caagcgcggc gatgattttg tcaccgtgac
     2101 tctggattac tatggcgggc tgcggtaccc gcacttggaa agagtagaag gaacgcccgc
     2161 tctcctcgat gcgatcttag cgccgtcgaa atagaagcaa aggcggccaa ccaccaattg
     2221 ctccggtatc ctcagccaat tttctggttt catcccgaaa acagtttcgc gcagcggtgc
     2281 cttaatcact ggtttgtcag caaatcggag ttggtcagaa aacgggcttc ggggacctca
     2341 gagcgcagct ggctgcaccg agcggacgtt tcctccaaga agacttcggg ggccttcgcg
     2401 tctcgtcggc cgcgaagtac cgatgcaatt cagttgccgc tttcgcatag gctgtccgtg
     2461 atccaagctc aatctcaaga gacgccagta ggcactcaag caactccaaa gttctaccgg
     2521 cccgcagggg ctgattcggg gccgatctgc gatgcacggg acgttagagg gacgcacgga
     2581 ctgctacgca attgatcccg gcgttacgat atctgtgtgg gaccaagcga cgaacgatat
     2641 gtgttcaccg ggaacaagtc gctcgccctt cgattggagg ctgggccgga aagggtacga
     2701 ttctcgggcc tgtaaggccc tcatgttgaa tcccactcat gatttgcgga aagaaactac
     2761 cgactgtggc gcacaatccg attggtcagc gcacgagtca acgaataagg catggctata
     2821 agaaccgggc cgacaatcag tgcggggccg gggaatactt catgcaagat cagaaattgg
     2881 gataacgcac ccagcaggat cgacatggcg atcaacacag agagtctctc gaaggcttct
     2941 ttcagcaacg ctcgccggcg ttggctgtcg aagagaacgc caaaaacgaa cggctcgcgg
     3001 cgggagcgcg catcggagat tcccgcccgt atgccgaaga acatggcgat ggcgggctgg
     3061 aagatgaacc ggaagcggcc agggcccttg agccgcttgg gaagtcttcc agaaactgac
     3121 gtgagaagag atagaagcca ccatgctgat acgcataccg taacgaaaaa gggatcgctg
     3181 cgaccaggac caagacgacc agagcgaaaa ccacgtttcg gattgtcagg gagcgtttca
     3241 cgtaacgaac ttagtccatc cgagagccat ccggctcgac cgttccacga tgttgaaaat
     3301 ctcgccggtc gtccaggcga aagcgaaggg ggccccagat tgccattaga gggctgagtc
     3361 cctccagttg taaggcagag ccatctcgcc ataaccgatg gtggtatagg tattggcgct
     3421 gaagcacatg gagtcccgaa tgttctcgac aaggccagta cccctgaggg cgacacccca
     3481 cacgcaaatc tcgagcacgt gcaggagcga gcatgaagag aatcacaccg gcgaagatgt
     3541 atgtcgccgg ttcggatgcc acgacctcca gcgcaatatc tcagactttc tctgatgacg
     3601 tgcgacgata tgatcgagcc cagggccatg aatcaggata atggtcacca gtatcacgcc
     3661 gccgaagccc aagacctcga tcggtacctg gaccggaata gccggctgga ccattttcat
     3721 gagaacgagc attgatggtt tattccgtgt ttgtcaccct acacgcttcg tagccgctgc
     3781 gcgggggtgc tccatccgtc acgcggtttc agcgcgagag ggtgggagcg aagcacgcgg
     3841 ttcctatcat ccatggtcat ttcggcatga acgagagcac accgacaacc gcgctgccca
     3901 gtaccagcag agctgggttg actttgtact ggagcaagat caatagaacg ccaaccgcaa
     3961 tcgctgccgt ttctgcaccg aagatcgccc ctttcgccat ggtgaagcag cccgccagca
     4021 gaagaccgat cgtgaccggc gcgagtccct gttgaatgga agttctccac aggctgccgc
     4081 ggagatatgg caccacgccg ccgaagctgg tggcgccgat gatcaggaac tcgaggaata
     4141 tctgaccaat tgagacttca gccggcactc tagtcgcgcc ggatcctgct tgcaactcgg
     4201 gttttgccgc agcgtcactc atggctttgg ggctcgttcc ccttgagacg gtaaaccatg
     4261 cctgtcttgg tcacgccgag cgcaagccaa tcctggatct cttgctgcaa cgttacgtag
     4321 aactgcgcta ccagcttctt ctgcttttcc gccacgtcct ctttgcgatc gaacttcacc
     4381 tggaaggcaa actctcccac cagcgacttg tgttcgccgc gcgtgcgcca caaccccatg
     4441 tcgcacttgc ctaccagtcc tttgccgaag tcgaactggc ccagcggaag cggtgcctct
     4501 tccacgatgc cttcattcac caggcttact ctctcgtcag gggacttctg cagggcggca
     4561 agcgccggaa atactttcac gagagtggcc atcgaggtct tgtctgcgtc gtgcatttgg
     4621 ctcactccaa attggcagtt gtgcgagtac agaatgcgat atccgccaac ttcgtttttc
     4681 agaggaagga cttcggcctt gaa
//
LOCUS       DASRKK010000468         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_330345, whole genome shotgun sequence.
ACCESSION   DASRKK010000468 DASRKK010000000
VERSION     DASRKK010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330345"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /locus_tag="VFC15_11000"
     CDS             <1..1963
                     /locus_tag="VFC15_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10727.1"
                     /translation="LLRRGSAVSAGIAIIAVPLIAWRTTPRADMFTVVLFAAFLSLLW
                     ENYQTGRARLWLLPLLMVAWVNLHFGFAAGLALLLAYVGVELSETIFGDVRRRAAMQR
                     LRHASGRLVCTALVTLVNPWGWGIYRALIRQQRAAGQQQLNIGEWSSVPVTWTAFSTA
                     LSLRQTQGAIYLLLAIAVVAAVLALLRRQLGAAILLLASTYPPVHHVRMGAVFACVVV
                     IVGGPVLAAAVVEFGSKIRQAPMRSTAAWAAVCLLAALALLRSFDLVTNRHYFRTVDE
                     STFGAGLGWWFPERAAEFIEREDLRGEIFNTYDEGSYLTWKLGPKRRDYIDGRDTLFG
                     VPRMQRAGQLLQISPDLEMWQQEADRYNINTIILPLARFDGVQLVQLKGFCDSREWRP
                     VYLDEISAVFVRRKPETEDLIQRLQVDCATATLPAAPLDYSAAGSFNQRANAADLLAA
                     LGRQTEALSATDSALAVFPDSPFVHWLRGNIFYAMGSRSDAEQEYIAAIALEPSEVAW
                     TALANLYQREGRILETIHAWQQASRLSSRPDLVQLKLAQFYLHIQQPKLTLQGLDEAV
                     RSAPADALAATGGSSLRFNVAQGRAAAWGALGDIKQATSFQEEAVKLAPDAADAWSHL
                     AKLYQRQGRFEDKYRAEERAAKLAGNPGR"
BASE COUNT          383 a          614 c          629 g          425 t
ORIGIN      
        1 gctactgcgc cgaggctccg ctgtcagcgc cgggatcgcc ataatcgcgg tacccctcat
       61 agcatggcgc accacgccgc gcgccgacat gtttaccgtc gtgctcttcg cggcgtttct
      121 gtcgctgctt tgggagaact accaaactgg ccgcgcgcgc ctgtggttgt tgccactgct
      181 gatggtggcg tgggtcaatc tgcacttcgg cttcgccgcg ggcctcgctc tgctattggc
      241 ctacgtgggc gtggaactgt cggaaacaat tttcggtgac gtgcgccgcc gcgcggccat
      301 gcaaaggctc cggcatgcct caggacggct ggtgtgtacg gcgctcgtga cgctggtaaa
      361 tccctggggc tgggggatct atcgggcact tattcggcaa cagcgagctg cggggcagca
      421 gcagctaaat atcggcgaat ggagcagtgt gccagtgact tggaccgcct tcagcacagc
      481 actttcactg aggcagaccc aaggcgcgat ctatctgctg ctggccatcg cggtggtcgc
      541 cgcggtgctg gctttgctga ggcgtcaatt gggagcggca atcctgctgc tcgcctccac
      601 ctacccgccg gtgcaccatg ttcgcatggg agctgtattt gcgtgcgtgg tggtgatagt
      661 cggcggacca gttctggctg cggcagttgt cgaatttggc tcgaagattc ggcaggcacc
      721 aatgcggtcc accgctgctt gggctgcggt ttgtttactg gcagcgcttg ctttgctgcg
      781 ctctttcgac ctggttacca accgtcacta ctttcggacc gtggatgaat cgacatttgg
      841 ggccggattg ggctggtggt tcccggaacg cgcggcagag ttcatcgaac gtgaggacct
      901 tcgcggagaa atctttaaca cctatgacga aggcagctat ctcacatgga aactcggccc
      961 gaaacgccgc gattatatcg acggacggga cacgctcttc ggagtaccac ggatgcagcg
     1021 agcgggtcag ttgctccaga tctcaccgga tttggagatg tggcagcagg aagcagaccg
     1081 ctacaacatc aacaccatca tccttccgct ggcgcgcttc gatggcgtcc agttggtgca
     1141 gctcaagggt ttttgcgaca gtcgtgaatg gagaccggtt tatctggacg agatatccgc
     1201 agtctttgtg cgtcgtaagc cagagacgga agatctgatc cagcgcttgc aagttgactg
     1261 cgcaaccgca actctgccag ccgctcccct cgactattcg gctgccgggt ccttcaacca
     1321 acgggccaac gccgcagatc tgctcgccgc tcttggccgc caaaccgaag cgctttcggc
     1381 gaccgacagc gcgcttgcgg tttttcccga tagccccttt gtgcactggc ttcgcggcaa
     1441 cattttctac gccatgggga gtcgctccga cgccgaacag gagtatatcg cggccatcgc
     1501 tctagaaccc agcgaggtcg cctggaccgc actcgccaac ttgtaccagc gagagggacg
     1561 aatcctcgag accatccacg cttggcaaca agcgagccgg ctgtcgtcca gaccagacct
     1621 cgtgcagctc aagctggcgc aattttatct gcatattcag caacccaagt tgaccttgca
     1681 aggactcgat gaggcggtcc gcagcgctcc tgccgacgcc ctcgcggcga caggcggaag
     1741 ctccctaagg ttcaatgtgg cacagggtcg cgcggccgct tggggagcgc ttggcgacat
     1801 caaacaagcc acatccttcc aggaagaggc ggtcaagctt gcgccggatg ccgccgacgc
     1861 ctggtcgcat ctcgcgaaac tgtaccagcg gcaaggaaga tttgaggata agtatcgggc
     1921 tgaggagcgc gctgcaaaac tggcgggaaa ccccggtcgc tgatgacgga ggagcttgtt
     1981 ctggatagag ccagccttca ggctggcgtc caagatcgcc ctcaccctaa ggctcaagcg
     2041 gttggtttgc c
//
LOCUS       DASRKK010000469         9183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_916446, whole genome shotgun sequence.
ACCESSION   DASRKK010000469 DASRKK010000000
VERSION     DASRKK010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916446"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..483
                     /locus_tag="VFC15_11005"
     CDS             133..483
                     /locus_tag="VFC15_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10728.1"
                     /translation="MDQPDHNFQMDYIEFPATDIAATKRFYSAAFGWSFTDYGPNYTS
                     FHDGRLGGGFTMSAQPVKGGALVVLYAKALEDTRRKVIEAGGKIVIEPFEFPGGRRFH
                     FADPNGNELAVWSE"
     gene            589..1929
                     /locus_tag="VFC15_11010"
     CDS             589..1929
                     /locus_tag="VFC15_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HZM10729.1"
                     /translation="MAGKLNFYLVKSIIVGALGGLLFGFDTAVIAGTTHQLTQVFNLS
                     AGQLGFTVSIALVGTIIGAMCSGVLGDLLGGRESLRILALFYVISALGCAFAWNWPSL
                     LIFRFIGGLGIGGSSVLGPVYIAEIAPAKWRGMLVGSFQINIVIGILVAYLSNFIIGR
                     FELGMSEWRWMLGVAALPAVLFLIMLFGIPRSARWLVTKNRIPEAREVLDRLGSPDGA
                     SELDDIIASIHVDGQVHSEPLFQRKYRLPIYLAFTVAAFNQLAGINAILYYLNYIFAM
                     AGFNKVSGDLQAVAVGAMNLVATLLGMSLIDKLGRKTLLMIGGFGMTFCLSGVAAVFY
                     TQQHQGLLVWLLVAYIGFFAISQGAVIWVYISEVFPNRVRSKGQALGSSTHWIMNALI
                     AFIFPVFVQRYGGAFPFVFFAVMMAVMFFVVMFTFPETKGVTLEEMQHKLGIEY"
     gene            1988..5386
                     /locus_tag="VFC15_11015"
     CDS             1988..5386
                     /locus_tag="VFC15_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HZM10730.1"
                     /translation="MKLSCRRVIFLVLLSSVIATAQSEPAADPYKPVLDQLQAITTVP
                     LANWQIVKADMPHGENPPASLVAAQSVTLKQDLQLPIWLYQSVEVPPTLNGYSLMGSH
                     VSLDLQISSNQGIQISVFVNGNMVARGDGDSQVPLTLLESAQPGQKLVVAVRVVSSGT
                     VGCCGGPAATSIYAASLQIQPANPRPDPAIFRQEVLAAELLIAAYPDGKAERQQQLDA
                     AVKAIDLGALDKGDQTAFDASLKAAQAKLDALKPYMSQFTIKSVGNSHIDMAWLWPET
                     ETVEVVRNTFGTALQLMREYPDFKFAGSAAQAYVWMEEKYPAIFHEIEQRVKEGRWEI
                     VGGMWVEPDLNMPDGESLVRQILYGKRYFKQKFGVDVNIGWNPDSFGYNAQLPQIYKR
                     SGIDYFVTQKLLWASEFTKFPYRLFWWQAPDGSRLMTYFPSTYSGQIDPVTTAQDSSV
                     YGPAMWKYNGGTNSAPDGALQMMYLYGVGDHGGGPTRVDLDTALRWQKPDVVFPKLEF
                     STAASFLNNLSKNESELNLPTWTGELYFQYHRGVQTTQSETKRGNRKGEVALLDAEKL
                     ASIGTLFGQAYPQADFDSSWKKVLFNQFHDVLPGSGIAINYVDAARKYAEVQRFSSDT
                     IHDALDGIASNISTVTWFRPPTPQERSAALKERQPIRENEWVSGEAIVIFNPLSWARS
                     GEVEQDLQLQFGADTYMMEHQSNAFVLEDEHGSVPFQILYGDPKKSKVRLRIFAREVP
                     ANGYTLIKVLPTTHNQNPIAWKSTLSATNTSLENEFLQVQVDPKTGCITSLFDKRSNT
                     EALAPAVAGVGAPANLPDGKPCGNLLQAFVDKPKKWDAWNVDADFIEHNTDLMQADDV
                     KLLESSPLRAVIRVRHRFQNSSFIQDITMYPSIPRVDVRTQVEWHEKHILLKVAFPLS
                     ARNDKATFEIPYGTVERPTTRNTPAEKAQFEVPALRWADLSDATHGFSLLNDSKYGYD
                     AKDNVLRLSLLRAPEYPDPNADQGHHEFTYSLYPHSGTWREAMTVRQGYEVNVPLLAV
                     TTTRHQGTLQASQSFFSTQEYNIVITAIKKDADDNSLIVRFYEWAGKKGDVHLTLPEK
                     ASAANATNLMEAVECQLSLDASGKVVTVPTNPYEIKTVKVQFAK"
     gene            5901..7811
                     /locus_tag="VFC15_11020"
     CDS             5901..7811
                     /locus_tag="VFC15_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZM10731.1"
                     /translation="MSFTLLDWSAIVGYLFITLVLGLWFRGRSGKSVDDYFVSGRNVN
                     WWLAGTSMVATTFAADTPLVVTGLVYTQGISGNWLWWGFLFSGMMTVFLFARLWRRSG
                     LLTDVQFAEIRYSGKPAAFLRGFRAIYLGLLMNCVILGWVTKAMTSIVSTTLANTPML
                     NNISGFMGGHFGSVWSGNDGAALAICVLFLIPFTGLYVALGGLWGVLWTDLFQFVLKM
                     SIVIAVAYYAIAACGGMSAVIASLQQMQARAGGNAESPLNFLPNFSRSVTSEPMWMVP
                     LANFFVFIGLQWWAFWYPGAEPGGGGYIAQRIFSARDEKQGLLSVLWFNIAHYAIRPW
                     PWILTGVAVIVLYPGLAHPETGYMLVLNNYLPHQYRGIAIAGFLAAFMSTIATQLNWG
                     ASYLVSDFYRRFVNKSATENHYVMVSRLCTVLLVIVAAWVSVQLGSIASGWEIVLQVG
                     AGTGAVYILRWYWWRINAWSEISAMASSLAISLVLSRWHPFRGNSSLVFAKGALATTV
                     VTSIVWVTVTLLTKPEPEEVLTRFYRNVRPDVRGWKPVAKLLPAIPANRDLGPNLGAW
                     LLGCAMVYTCLFGTGKVLLHQPAIGVALLVVSAVCAFLLYHRVVRNFAVESQEPEGSV
                     PDWVETPATTGH"
     gene            7883..8314
                     /locus_tag="VFC15_11025"
     CDS             7883..8314
                     /locus_tag="VFC15_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10732.1"
                     /translation="MAANVQIEANLLQVVPFLGVADMDRSVRFYIDNLGFTIKHQWTP
                     EGKLRWCWLTRGGASLMLQEYLAGRRPEGVLGQGVSLCFQCEDAVALYHEFRSRGLEA
                     AEPFVGNSMWVTVPSDPDGYKLDFESVTDTPEDTKLSELEG"
     gene            complement(8343..8936)
                     /locus_tag="VFC15_11030"
     CDS             complement(8343..8936)
                     /locus_tag="VFC15_11030"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZM10733.1"
                     /translation="MGTDRIETIKEYFGATMTAMICLLRGVNVVGSNQIKMDALRDLC
                     VSLKLCRAQTYIQSGNVVFQTDERDHAKLAARIESAIEKNHGFRPAAVFRTAAEMREV
                     IARNPFDSRKRIEPGKLIVTFLATEPAAETQKALLALEPNPEEMHVKGREIYVYFPDG
                     MGRSKFAGLLARKLKNTGTARNWNTVMKLLEMAEKLE"
     gene            complement(8964..>9183)
                     /locus_tag="VFC15_11035"
     CDS             complement(8964..>9183)
                     /locus_tag="VFC15_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZM10734.1"
                     /translation="FGNVVDLLEPDIIVVGGGLSSLMARWFDRIASQLPFWSVNARCG
                     EIPFAEAKYRADSGIAGAAALCVARALL"
BASE COUNT         1824 a         2668 c         2678 g         2013 t
ORIGIN      
        1 ggcagccacg gccattgtgc aaccctgcga gcctcggcag cgcgctgaat tcggcgagcc
       61 aaaggtcacc ctgcatgcag tctaaagccg gtgtgctaat cttgcccgcg gatacccaac
      121 acgaggttcg acatggatca accagaccac aactttcaga tggactacat tgagttcccc
      181 gccaccgaca ttgcggcgac caagcgcttc tacagcgcgg cgtttgggtg gagcttcacc
      241 gactacggcc cgaactacac cagcttccac gacgggcgtt tgggcggagg ttttaccatg
      301 agtgcccaac ccgtgaaggg cggcgcgctg gtcgtgctgt atgccaaggc gttggaagat
      361 acgcggcgca aggtcattga ggctggcggg aagatcgtga tcgagccatt tgagtttccc
      421 ggcgggcggc gcttccactt tgccgatccg aatggaaacg aactggctgt ctggagcgag
      481 taagcagaga ttcgaacaac atggatggtc ttgtggcgaa caaagccaca ggcgtgtgta
      541 gcctcgcatt cccatagacg aagtccgagt tcattggagg cgtagagcgt ggcaggcaaa
      601 ctcaattttt acctggtgaa gagcattatc gtcggggcct tgggtggcct gctgttcggt
      661 tttgacaccg cggtcatcgc cggaaccacc catcagttga cgcaggtttt taacctctcg
      721 gctggtcaat tgggctttac cgtctcgatc gccctggttg gcaccatcat cggagctatg
      781 tgttcaggag tcctcggtga tcttctgggc ggacgggaga gcttgcgcat tctggctctc
      841 ttctacgtga tctcagcgct cggttgtgcc ttcgcatgga actggccttc cttgctgatc
      901 ttccgcttca tcggcgggct cgggatcggt ggctcgtccg tgctcggccc cgtgtacatc
      961 gcggaaatcg ctcctgcaaa gtggcgaggg atgctggtcg gctcgtttca gatcaacatt
     1021 gttatcggaa ttcttgtcgc ctacctgtcc aacttcatta tcgggcgctt tgagttgggg
     1081 atgagcgaat ggcgctggat gttgggtgtt gcggcgctcc ccgcggtgct ctttctcatc
     1141 atgctgttcg ggattccgcg ttcggcacgc tggctggtaa ccaagaaccg catccccgaa
     1201 gcgcgcgaag tgcttgatcg gctgggttcg cccgacggag ccagcgaact ggacgacatc
     1261 atcgcctcga tccacgttga cggccaggtg cactccgagc cgctcttcca gcggaagtac
     1321 cgcctgccca tttatctcgc cttcaccgta gccgcattca atcagcttgc cggaatcaac
     1381 gccattctct actacctgaa ttacatcttt gccatggccg gtttcaataa ggtctccggc
     1441 gatttgcagg cggtggcagt cggagccatg aacctggtcg ccactctatt ggggatgtca
     1501 ctcatcgata aactcggtcg caagacgctg ctgatgatcg gcggctttgg catgacgttc
     1561 tgcctgagcg gtgtggccgc ggtgttctat acccaacagc accagggctt attggtgtgg
     1621 ttgctggtcg cctatatcgg gttcttcgcc atctcccaag gcgcggtcat ctgggtctat
     1681 atcagcgagg tctttcccaa tcgcgtgcgc tccaaagggc aggccctggg cagttcgacc
     1741 cactggatca tgaacgcctt gatcgcgttt attttcccgg tgtttgtgca acgctacggt
     1801 ggcgcgttcc cgtttgtatt cttcgccgta atgatggctg tgatgttctt cgtggtgatg
     1861 ttcaccttcc ccgaaaccaa aggcgtcacg ctggaagaga tgcagcacaa gctgggaatt
     1921 gaatactaac ggcagtgtgc ccgccggggt aacgttccgg cgggtgctgc tgctttgaag
     1981 gagcgtcatg aaactctcct gccgtcgcgt tattttcctc gtcctacttt cttccgttat
     2041 cgccactgcg caatcagaac ctgccgccga tccttataag ccggtactcg accagctgca
     2101 ggcgatcacg acggttccgc tggcgaactg gcagatcgtg aaggccgaca tgccgcacgg
     2161 tgagaatcca ccagcctcat tggtcgcggc ccagtccgtg acgttgaagc aggaccttca
     2221 actcccaatc tggctgtacc agagcgtcga ggtcccgccc acattgaatg gatactcgtt
     2281 gatgggctcc cacgtatcgc ttgacctgca gatcagcagc aaccagggca tccagatttc
     2341 ggtgtttgtg aatggcaaca tggttgcacg cggggatggc gactcgcagg ttcccctgac
     2401 gttgcttgaa agtgcgcagc ccggacaaaa gctggtcgtt gcggtgcgag tcgtgtcctc
     2461 aggcacggta gggtgctgcg gcggcccggc ggccaccagc atttacgccg cttcgctgca
     2521 aatccagccg gccaacccgc gtcctgatcc ggcgatcttt cgtcaagaag tgctagcggc
     2581 cgagttgctc atcgccgcat atccggacgg taaggctgaa cggcagcagc aactcgatgc
     2641 tgctgtgaaa gccatcgact tgggcgcgct cgacaaaggc gaccagacag ctttcgacgc
     2701 ctcgctcaag gcggcgcagg caaagctcga cgcactcaag ccttacatga gccagttcac
     2761 gatcaagtcc gtgggcaaca gccacatcga catggcgtgg ctgtggccgg agactgaaac
     2821 cgtcgaggtg gtgcgcaaca ctttcggaac agcgctgcaa ttgatgcgcg agtatcccga
     2881 cttcaagttc gctggatcgg cggcgcaggc ttatgtctgg atggaggaaa aatatccggc
     2941 gatcttccac gaaatcgagc agcgggtgaa agaagggcgc tgggaaatcg tcggcggcat
     3001 gtgggtcgag cccgacctca acatgcccga tggcgagtcg ctggtgcggc aaatccttta
     3061 cggcaagcgc tacttcaagc agaagtttgg cgtggacgtg aacatcggct ggaacccgga
     3121 ctcgttcggc tacaacgccc agttgcctca aatctacaaa cgctcgggga tcgattattt
     3181 cgttacgcag aaactgctgt gggcctcaga gttcaccaag ttcccctacc gcctcttctg
     3241 gtggcaggcg cccgacggct cgcgcctgat gacgtatttc cccagcacgt atagcggcca
     3301 gatcgatccg gtaaccacgg cgcaggattc ttcggtctat gggccggcga tgtggaagta
     3361 caacggaggc acaaactcag cgcccgacgg cgccttgcag atgatgtatc tctacggcgt
     3421 gggcgaccat ggaggcggac ccacacgggt cgatctggat actgcgttgc gctggcagaa
     3481 gcccgacgtg gtgtttccca agctggaatt ctcgactgcg gcgagcttcc tcaacaacct
     3541 cagcaaaaac gagagcgagc ttaaccttcc cacgtggacg ggcgagttgt acttccaata
     3601 tcaccgcgga gtgcagacca cgcagtcgga aaccaagcgc ggtaaccgca agggcgaagt
     3661 cgcgctgctc gatgccgaga agctcgcgtc gatcgggacg ctgttcgggc aggcctatcc
     3721 gcaggcggat ttcgattcgt catggaagaa ggtgctgttc aaccagtttc acgacgtctt
     3781 gccgggctcc ggaattgcca tcaactatgt ggatgcggcg cgcaagtacg ccgaggtaca
     3841 acggttcagc agcgacacaa tacatgatgc gctcgacggc atcgcttcga acatcagcac
     3901 agttacctgg tttcgcccgc caacgcctca ggaaaggtct gcagcgctga aggaacgcca
     3961 gccaatacgc gagaacgaat gggtttctgg cgaggcgatt gttattttca acccgctttc
     4021 ctgggcgcgg tccggcgagg tcgaacaaga cctgcaacta cagtttggtg cagataccta
     4081 catgatggaa caccaaagca atgctttcgt actcgaagat gaacatggat ccgtgccgtt
     4141 tcagattttg tacggcgatc ccaagaagtc taaagttcgc ttgcgaatct ttgcgcgaga
     4201 ggttcccgca aatggttata cgctgatcaa agtcttgcca acaacacaca atcagaatcc
     4261 gattgcctgg aagtcgacac ttagcgctac taacacatcg ttggagaacg aattcctgca
     4321 ggtgcaagtc gatcctaaga ccggatgcat cactagcctc ttcgacaagc gcagcaacac
     4381 cgaagccctt gcgcccgcgg tcgcaggcgt gggcgcgcct gccaacctcc ccgatggcaa
     4441 gccctgcggc aacctgctgc aagccttcgt ggataaaccc aagaagtggg acgcctggaa
     4501 cgtggacgcc gacttcatcg agcacaacac cgacttaatg caagccgacg acgtcaagtt
     4561 gctcgaaagc tcgccccttc gggctgtgat ccgcgtgcgg cacaggttcc agaactccag
     4621 cttcattcaa gacattacga tgtatccgag cataccgaga gttgacgtgc gcacgcaggt
     4681 tgaatggcat gagaagcaca tcctgctgaa ggtcgcgttc ccgctgagcg cgcgcaacga
     4741 caaggcgacg tttgagatcc cgtacggcac ggtcgaacgc cccaccacgc gcaacactcc
     4801 tgcggagaag gcgcagtttg aggtgcccgc gctgcgttgg gctgatctgt ccgacgcgac
     4861 gcacggcttt tctctgctga acgacagcaa gtacggatac gacgcgaaag acaacgtgtt
     4921 acggctttca ctgctacgcg cgccggagta tccggacccg aatgccgacc agggccatca
     4981 tgaattcact tattcgctct atcctcattc tggcacctgg cgcgaggcga tgaccgtgcg
     5041 acagggctat gaggtcaacg tgccgctgct ggctgtgacg accacacgcc atcaaggcac
     5101 tctgcaagcg tcgcagtcgt tcttctccac gcaggaatac aacatagtga tcacggcaat
     5161 caagaaggat gccgacgaca actcgctcat cgttcgcttc tacgagtggg ccgggaagaa
     5221 aggcgatgtg catctaacgc tgcctgagaa ggcgagtgca gccaacgcga cgaacttgat
     5281 ggaagctgtc gaatgccagc tcagtctgga cgcgagcggc aaagtcgtca ccgtcccaac
     5341 gaatccctac gagatcaaaa cggtgaaagt acaattcgca aagtaggcag gctgccggat
     5401 cgaatgaaat agggtgagcc cgccttcaca ggctgcggaa gtcgatgttc tggggagagc
     5461 cggcccttgg agcctgcctt ggttactttc gtttcttgct gctcccatat tctcttgcgt
     5521 ttgccctccg tcccagttac aaccaactct gtgaagctgg aggaacgtgc gcctctagct
     5581 ggctcgcagc gatgccgcga atgtaaatcg ctctttgaca attcctgaat tggagcggaa
     5641 gtgtgctgac ctgcgactat aaaacaccag ctaagtccca cgcgcagata tggatacgtg
     5701 taagtagatt atggaatcaa ttacatgcgg acaatttcag tctaaatcgc tgattctaaa
     5761 cgatcgtgat ttttattttg cagtgttata cacgactata tcaccccacc tttgcgccgt
     5821 cggaacattg ctccggtcca ctcgtgctca tctgtcgctc ttttttgctt ttctctacgc
     5881 ttgaacgata attgccgcgt atgtctttta ccctactcga ctggtcggcc atcgtaggtt
     5941 atctcttcat cactcttgtc ctcggcttgt ggttccgcgg ccgctccggc aagagcgttg
     6001 acgactactt cgtctccgga cgcaatgtga actggtggct ggcgggcacg tcaatggtgg
     6061 ccacgacctt tgccgccgat acgcccttgg tggtcaccgg actggtatac acgcagggca
     6121 tctcgggcaa ctggctgtgg tggggcttcc tgttttcggg gatgatgacg gtctttctct
     6181 ttgcgcggct gtggcggcga tctggcttgc tgaccgatgt gcaattcgcc gagatccgtt
     6241 actcgggaaa gccggcggcg tttctgcgcg ggttccgcgc catctatctc gggctgctga
     6301 tgaactgcgt cattctcggc tgggtcacca aggcgatgac ctcgatcgtc agcaccacac
     6361 tggccaacac tcccatgctg aacaatatca gcggcttcat gggcggccat ttcggctcgg
     6421 tgtggagcgg caatgatggc gcggccctgg cgatctgcgt gctgttcctg atcccgttca
     6481 ctggcctcta cgtcgcgctc ggaggcttgt ggggcgtgct ctggaccgac ctgttccagt
     6541 tcgtgctgaa gatgagcatc gtgattgcag tggcctatta cgcgatcgca gcctgcggag
     6601 gcatgagcgc ggtgattgcc agcctgcagc agatgcaagc gcgcgcgggc ggaaatgccg
     6661 agagtccgct gaacttcctg ccgaacttct cgcgcagtgt gacctcggag ccaatgtgga
     6721 tggtcccgct ggccaacttc ttcgtcttta tcgggctgca atggtgggcg ttctggtatc
     6781 ccggagccga gccgggcggc ggcggctaca tcgcgcagcg catcttcagc gcgcgtgatg
     6841 agaagcaggg cctgctgtcg gtgctgtggt tcaacatcgc ccactacgct atccggccat
     6901 ggccctggat cctcacgggc gtagcggtga tcgtgctcta tcccgggctg gcgcatcccg
     6961 agaccggata catgctggtg ctcaacaatt acctgccgca ccagtatcga ggtattgcaa
     7021 ttgcgggatt tcttgccgcc ttcatgtcga ccattgcgac gcagttgaat tggggcgcgt
     7081 cgtacctggt gtcggacttc taccggcgct tcgtgaacaa gagcgcaacc gagaaccatt
     7141 acgtaatggt ctcgcggctc tgcactgtgc tgctggtgat cgtggcagcg tgggtttcgg
     7201 tgcagctcgg ctcgatcgct tcgggctggg aaatcgtgtt gcaagtcggc gcggggacgg
     7261 gcgcggtata catcctgcgc tggtactggt ggcgcatcaa cgcgtggagc gagatttccg
     7321 cgatggcgtc atcattggcg atcagcctgg tgctgagccg ctggcatccg ttcaggggca
     7381 atagctcgct cgtctttgcc aagggagcgc ttgcgaccac cgtggtgact agcatcgtgt
     7441 gggtcactgt gacgctgctc accaagcccg agccggaaga agtgttgacg cgtttctatc
     7501 gcaacgtgcg gcccgacgtt cgcggatgga agccggttgc aaagctgctt ccggcgattc
     7561 ctgctaaccg cgacctcggg ccaaatctcg gagcatggtt gttgggctgc gccatggtgt
     7621 acacctgcct gtttggcacg ggcaaggttt tgctgcacca accggcgatc ggggtcgcgt
     7681 tgttggtggt gtcggcagtg tgcgcgttcc tgctgtatca ccgagtggtg cgcaatttcg
     7741 cggtagaatc ccaggagccc gaaggcagcg tgcctgactg ggtggagacg ccggcaacaa
     7801 cggggcactg aagaaattta gccactgaat tgcacggatc agtatccgta agatttgggc
     7861 tctcaactga tcggaggacc agatggcagc aaacgtccag atcgaagcca accttttgca
     7921 ggttgtacca tttctcggcg tcgccgacat ggaccgctcg gtgcgcttct acatcgacaa
     7981 cctcggcttc accatcaagc atcaatggac accggaaggc aagctgcgct ggtgctggct
     8041 cacgcgcggc ggcgcgtcgc tcatgttgca ggaatatctc gccggccgcc ggcccgaggg
     8101 cgttctgggt cagggcgtat cgctctgctt ccagtgcgaa gacgctgtcg cgctctacca
     8161 tgagtttcgc tcacgcggcc tcgaagcagc ggagccgttc gtgggcaatt cgatgtgggt
     8221 aactgtgccc agcgatcccg acggctacaa gctcgacttc gaaagcgtca ccgacactcc
     8281 agaagatacg aaactctccg agttagaggg ctgaacaact tggctacgcc ggatcgacct
     8341 gtctactcga gcttctccgc catttccagc aatttcatga cggtgttcca gtttctggcg
     8401 gtgccggtgt ttttcaattt tcgcgcgagc aatccggcga acttggagcg tcccattccg
     8461 tcgggaaagt acacatagat ttcacgcccc ttcacgtgca tttcttctgg attcggttcg
     8521 agcgcgagca acgctttctg cgtctcggcc gcgggctccg tcgcgagaaa cgtaacgatc
     8581 agtttgccag gctcgattct tttgcgactg tcgaatggat ttctggcaat cacctcgcgc
     8641 atctccgccg cggtacgaaa caccgcggcg ggccgaaagc catgattctt ctcgatcgcg
     8701 ctttcgatcc gcgccgctaa cttcgcatga tcgcgctcgt cggtttggaa gacgacattt
     8761 ccactttgga tgtaggtctg cgccctgcag agtttcagcg atacgcaaag gtcgcgcagc
     8821 gcatccatct tgatctggtt gcttccgacg acattcactc cacgaagaag gcaaatcatc
     8881 gcggtcatgg ttgctccaaa atactccttg atcgtctcaa tccgatcggt gcccatccgc
     8941 gcagtccatg gatgaccttc tcttcacagc aacgccctcg ccacacacag agccgccgcg
     9001 cccgcgatgc ccgagtcggc gcgatacttg gcttcggcaa acggaatctc gccgcaacgc
     9061 gcattgaccg accagaaggg caactgcgag gcgatgcggt cgaaccatcg cgccatcagc
     9121 gaactcagcc cgccgccgac cacgatgatg tcgggctcca gcagatcgac cacattgccg
     9181 aac
//
LOCUS       DASRKK010000470         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1112373, whole genome shotgun sequence.
ACCESSION   DASRKK010000470 DASRKK010000000
VERSION     DASRKK010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112373"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFC15_11040"
     CDS             complement(<1..211)
                     /locus_tag="VFC15_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10735.1"
                     /translation="MAGEHNTPIGTPILMAPGKIFLVGEYAVLDEGSAVLAAIGRYAR
                     AQFIPRAQSMRPLVAAVVERAKEHLD"
     gene            complement(332..1726)
                     /locus_tag="VFC15_11045"
     CDS             complement(332..1726)
                     /locus_tag="VFC15_11045"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10736.1"
                     /translation="MSKAKISSREERVTPVALPVLRPLVAGIDLGSSQHWVCGPAHDD
                     GKANVRVFGTTTAQLHDLADWLVKEGVQSVAMESTYVYWIPVYEVLASRGLQVVLVNA
                     RQLHNVPGRKTDFLDCQWIQVLHSCGLLRGSFRPAEAITQMRAMQRQMANLVAEQTRC
                     VQWMQKALDQMNVQVHRAVTDITGTTGMSIIRAIVAGERDPKQLAKYRDQRCRKSVED
                     IEMHLVGTWREEHLFNLASALRMYDAFGQEIAAYETHLIKALEALQPPERRDQAVPPH
                     PNRAKEKAIRKRNEQELRTGLWRFAGVDLTRIDGISSGSARVVLTELGPDLAGFPSES
                     NFVSWLRLCPRVPISGGKPIKKRRNGLGANRIAGVLRMAATSLRGSKSALGAFYRRHS
                     RHNGADVAVFATARELAKLIYRMLRFGQDYVDVGERAYESRFELRRVASLKEAAKSLG
                     FALIPASPVEAAQG"
BASE COUNT          381 a          615 c          628 g          442 t
ORIGIN      
        1 catcgaggtg ctccttcgct cgctcgacga ccgccgcaac gagtggccgc atgctctggg
       61 cgcgtgggat gaattgcgcg cgggcgtagc gaccgatggc cgccaagacg gcagagcctt
      121 cgtccagcac ggcatattcg cccactagaa agatctttcc tggcgccatg agaatgggcg
      181 tcccgatagg tgtgttgtgt tcaccggcca tggtgtctcc agcgacgagt cttgcggtcg
      241 aagtcggccg agatcggcgt gagaagccaa catcggcaga tgatagctac gacttcatcg
      301 aagggttgta atggaaaccc tagcctgaaa ctcacccctg ggcggcttcg actggtgagg
      361 ccggaatcag ggcaaagccg agagatttcg cggcttcttt gaggctggca accctacgga
      421 gttcgaagcg gctctcgtac gctcgctcgc ccacgtcgac gtagtcttgg ccgaagcgga
      481 gcatgcggta gatgagcttg gccagttctc gagcggtagc gaacacggcg acgtccgccc
      541 cattgtggcg cgagtggcga cggtagaacg ctccgagtgc gctcttggat cctcttaggg
      601 acgtggctgc catgcgtaga acaccagcga tccgatttgc gcccaggccg ttgcgccgct
      661 tttttatcgg cttgccgcct gaaatgggaa ctcgtgggca tagccgcagc caggagacga
      721 aattgctctc cgacgggaat ccggccagat ccggacccaa ctccgtcaga actacgcgag
      781 cggatcctga gcttatgccg tcaattcgtg tcaggtcgac gcctgcgaag cgccaaaggc
      841 cggtccggag ttcttgctca ttgcgttttc gaatggcttt ctctttggct cgattcggat
      901 gcggcggaac cgcctgatcg cggcgctcgg gcggctggag tgcttcgagc gccttgatga
      961 ggtgggtctc gtacgcagcg atttcttgcc caaaggcgtc gtacatccgc agagcggacg
     1021 cgaggttgaa gaggtgctct tctcgccaag tccccacgag gtgcatctct atgtcttcga
     1081 ccgatttgcg acatctttgg tcgcgatact tcgccagttg cttcggatcc ctctcaccag
     1141 cgacgatggc acggataatc gacatcccgg tcgtaccagt gatgtccgta acagcgcgat
     1201 gaacctgcac gttcatttgg tccagggctt tctgcatcca ctgcacgcaa cgggtctgct
     1261 cagcaaccag attggccatc tgccgttgca tggcccgcat ctgcgtgatc gcctcggcgg
     1321 gccgaaagga tccgcgaagc agaccgcagc tatggagcac ctggatccac tggcaatcga
     1381 gaaaatctgt ctttctgcct gggacgttgt gtaattgccg agcgttcaca agcaccactt
     1441 ggagtcctcg cgacgcaagc acctcgtaca ccggtatcca gtagacgtac gtgctttcca
     1501 tcgcgacaga ctggacgcct tctttgacga gccagtcggc caagtcatga agttgtgccg
     1561 tggtcgtgcc gaacactcgg acgttggctt tgccgtcgtc gtgcgcaggc ccgcagaccc
     1621 agtgttggga gctgccgagg tcgattcccg cgaccagtgg tcgaagtaca ggcagcgcta
     1681 ccggagtcac cctctcttct ctgctggaaa tttttgcttt ggacatgtat ccaccttgtt
     1741 gaaggcgatg tcctcggccc gagatggctc gtttgtgtcg actctagcct gggggagtgc
     1801 atgcggctgg caccgcttgc ctcaccaatg aatgatggcc tgctcggacc cagactgacc
     1861 taggggcaca tggctccaat gattcgaagg ggtatggctg ccgaggacac gccttctcct
     1921 acatgccaga tctgctcacg agtttcaccc tcgtatgatc cggcggaacg ccgggggccc
     1981 cgctgacctg aaacttgccc ggccgcgcca agtttcacgc ttgaaggatc cgggctctcg
     2041 gcccggcggc ggcgctcaaa gggttt
//
LOCUS       DASRKK010000471         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1014816, whole genome shotgun sequence.
ACCESSION   DASRKK010000471 DASRKK010000000
VERSION     DASRKK010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014816"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFC15_11050"
     CDS             <1..1233
                     /locus_tag="VFC15_11050"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZM10737.1"
                     /translation="NLRAQAFTGAYGSTEQDATSEVRNNTVLVESNNPLSMRGGLTAD
                     VYIGKGVLTQPSKGTQAMWFLRSNPIVLLPLWAFVVMFFFWWTKGRDPHPDISVAPMY
                     EPPKDLTPAETGTLIDDVVHPRDITSTMVDLAVKGYLKIEETESKVLLFTHRDYTFHA
                     LKPPDTWSSLEPHERVMLNHMFTGGATQVRLSDLKNQFYVAIPTMKNDILAELKSKGM
                     YSVDPESAHAYVLAGILFTAAPFVLAQVLGWGSMLDSPGLLIASGIIAVIIVFLFARI
                     MTAKSLKGVRTKVEILGFQEFINRVDADRLKRMPPDTFEKFLPYAMALGIENRWAKAF
                     QGIAQNQPTWYVGPTPYVGFNPIFFAGSMHAMATDAHQAFVAAPRASSTGSGWGGGGF
                     GGGGFSGGGFGGGGGGAF"
     gene            complement(1361..1642)
                     /locus_tag="VFC15_11055"
     CDS             complement(1361..1642)
                     /locus_tag="VFC15_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZM10738.1"
                     /translation="MRNQRERRYSVYILGSLSGTLYIGITSNLPFRVRQHKEHTFRGF
                     TTKYRVDRLLYYEAYGEVSTAIAREKQLKGWKRERKIALIEEATRSGRI"
BASE COUNT          426 a          623 c          637 g          459 t
ORIGIN      
        1 aacttgcgcg cgcaagcctt caccggcgca tacggatcga cggagcagga cgctacctcc
       61 gaagtccgca acaacaccgt gctggtcgag agcaacaatc cgctctccat gcgcggcggg
      121 cttactgccg acgtttacat cggcaaaggc gtgctgacgc agcccagcaa agggacccag
      181 gcaatgtggt ttctgcgcag caacccgatt gtgctgctgc cgctgtgggc gtttgtggtg
      241 atgttcttct tctggtggac gaagggccgc gatccccatc cagacatttc ggtcgcgccc
      301 atgtacgagc cgccgaaaga cctgacgccc gccgaaaccg gtaccctgat cgacgatgtc
      361 gtccatcctc gcgacatcac ctcgaccatg gtggacctgg cggtgaaggg ctatctcaag
      421 atcgaggaga cagaatccaa ggtcctgctc ttcacccatc gcgattacac cttccacgcg
      481 ctgaaaccgc ctgacacctg gagctcgctc gaaccacacg agcgcgtgat gctgaaccac
      541 atgttcactg gcggggccac tcaggtgcgc ctgtcagacc tgaagaacca gttctacgtc
      601 gctatcccga ccatgaagaa cgacattctc gcggagttga agagcaaggg aatgtactcg
      661 gtcgatccgg aatcggcaca tgcctatgtg ctggcgggaa tcttgtttac ggcggcgccc
      721 ttcgtactgg cccaggtcct gggctggggc tcgatgctgg attcgccggg cctgctgatt
      781 gcatcaggca tcatcgcggt gattattgtc ttcctgttcg cgcgcatcat gaccgcaaag
      841 tcgcttaagg gcgtgcgcac caaagttgag atcctgggct tccaggagtt catcaaccgc
      901 gtggacgccg atcgcttgaa gcgcatgccg cccgatacct tcgagaagtt cctgccttac
      961 gccatggcgc tgggcattga gaaccgctgg gccaaggcgt tccagggaat agcgcagaac
     1021 cagcccacat ggtatgtggg accaacgccg tatgtgggat tcaatcccat cttcttcgcc
     1081 ggctccatgc atgccatggc gacggatgcg caccaggcct tcgtggccgc accgcgcgcc
     1141 agcagcaccg gttcaggatg gggcggcggc ggcttcggtg gtggaggctt cagcggcggc
     1201 ggcttcggcg gcggaggtgg tggagcgttt tgaaggcagc gtaatttttt tgtttgtcat
     1261 cctgagcgag gagcggagcg ccgagtcgaa ggacccctat cgccgctcaa atctatcctt
     1321 agaaacgtgg ctcttgctgg cttctcgtac cacccgtggc tcaaatcctt ccactgcggg
     1381 ttgcctcttc gatcagtgca atctttctct cccgcttcca acccttcaac tgtttttcgc
     1441 gagcaatggc tgtcgaaacc tccccatagg cctcgtaata gagcaagcgg tcaaccctat
     1501 acttggtggt gaatccacga aacgtgtgct ctttgtgctg acgcactcga aagggcagat
     1561 tgctggtgat gccgatgtac agcgtgccgc tgaggctgcc gagaatgtaa accgaatagc
     1621 gacgctcgcg ctggttacgc aaggtttctg acgactatag gggtccttcg ctgcgctcag
     1681 gatgacagga tgggggtttt ggggcggtga tgcataacct cagctaacgt gatgtcgtac
     1741 acgcgccacg tcggtcatct tgagcgacga gcgcagcgag gagtcgaagg acccctatgg
     1801 ccgacaggaa tctcggactt gttggtgcgc cacgcaacca cactgtgatt tctcgcagct
     1861 ataggggtcc ttcgactcgc tgcgctcgct caggatgaca aacagaaaaa ccggtaatta
     1921 tgtcaacggc agacgaggaa ccgcgaggat ctgtctcttc gccagggtta ggtcttgagc
     1981 aagcgagcga tggtttcgag cagcacttca ggagcttcgc ctttgggtaa gaactggtcg
     2041 gcaaattcga ttccctcggg tgtctccgcg gccgccgaaa cgataaggat gggtacgtgc
     2101 ggcttgatgg tcttcatctc ccgggcaatc tcggtaccgg ttttg
//
LOCUS       DASRKK010000472         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_330652, whole genome shotgun sequence.
ACCESSION   DASRKK010000472 DASRKK010000000
VERSION     DASRKK010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330652"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1010)
                     /locus_tag="VFC15_11060"
     CDS             complement(219..1010)
                     /locus_tag="VFC15_11060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZM10739.1"
                     /translation="MIRWKTFAIGCSLALALLLIAQHKPAMSDPNVYRAVIDLTHNLN
                     GHSPNWEGSEESPFQAKELGNIEHDGYYSRVFTTQEHYGTHLDAPAHFAKGMWTVDQI
                     PVERLVRPLVVLEVRSKARSNPDYEISVQDIADWEFAHGEVPTGAVVMAYTGWDERWN
                     SQKEFRNVGSDHLSHYPGFSLEAVKFLVKTRNVVGLGIDTMSVDIGATTTYPVHLFTS
                     KQSVFHLENVANLGLVPPAGATVVVAPIKLEGGSGGPARVLALVK"
     gene            complement(1210..>2117)
                     /locus_tag="VFC15_11065"
     CDS             complement(1210..>2117)
                     /locus_tag="VFC15_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10740.1"
                     /translation="APNAFRVQNGIIKGEVLGGWLKRLALLFDRIAIYDLAPALSQME
                     DSRLAEELRWLLTKEVICQPEVEDREYENVTEEYQALSRAAVHELSETILSISNSLEK
                     FEAEQPDEKAILREVADSLELATWAQTRSAALWFRAVDGTAVIPVIERIPQPMSNSDP
                     GVPTVQIMIKAFPVLDDSTRWEDVIEFRSAPENRRALAALRNWANETARLRLQPHEIL
                     DKLETLLSQYQSQLELNRLKFKLGTMEVIVTATAGVVEELINLNFGKAVKAALEFKQR
                     RVELLQAEINAPGSELAYIVRASTL"
BASE COUNT          455 a          610 c          568 g          484 t
ORIGIN      
        1 ctgctaacgc ggacctgaag gtccgcacta cccggtcagg tctacgctaa ctctgaaacc
       61 gctcagcccg agccgaggga tctgcggtcc tcatcgtgag caacggcagg cctctcgact
      121 gcgtgcgatc gctagcagag acctcactcc gctcgggatt acagtgctaa cgtagtcgcg
      181 aacagcgaac agcgaatggc gaacagcgaa cagcggcctt acttcaccag cgccagcacc
      241 cgcgccgggc caccggaacc gccttccagc ttgatgggcg cgaccaccac cgtcgctccg
      301 gccggtggaa ccagcccgag gttggcgacg ttttccagat ggaaaacgct ctgcttcgaa
      361 gtgaacagat gcaccggata ggtcgtggtt gcgccgatgt ccacgctcat ggtgtcaatg
      421 ccgagcccca ccacgttgcg cgtcttcacc aggaacttca ccgcctccag cgagaaaccg
      481 gggtagtgcg acagatgatc gcttcccacg ttgcgaaact ctttttgcga gttccagcgc
      541 tcatcccaac cggtatacgc catcaccacg gcgccggttg gaacttctcc atgtgcaaat
      601 tcccagtcgg caatgtcttg cacgctgatt tcgtagtcgg gattacttct ggccttgctt
      661 cgaacctcca gcaccaccag cggacgtacc agcctctcga ccgggatctg gtccaccgtc
      721 cacatgccct tggcgaaatg cgcgggcgcg tcgaggtggg tgccgtaatg ttcctgggta
      781 gtgaagacgc gcgagtaata tccgtcgtgc tcgatgttgc ctagttcctt cgcctggaac
      841 ggagactcct cgctgccttc ccagttgggc gaatggccgt ttaggttgtg ggtgagatcg
      901 ataaccgcgc gatagacatt cggatcggac atggcaggtt tgtgctgcgc gatcaacaac
      961 agcgccagcg ccagactaca gcctatcgca aatgtcttcc acctgatcat cgcaaaacaa
     1021 ctccctgcaa aatatccgat gctccgaagg gagcgatcgc ttcgtgaatt atgtttaaca
     1081 aagagagaac ccgggccgtc acctttgatg cgggcggggg ataaatcgca gttgtgcgtg
     1141 gtgaaccgca gatcccctcg gctgcgctcg gggcaggggc acttcgactc gccaatgtcc
     1201 accgcataac tagagtgtgc tggctctaac tatgtatgca agttcggagc ctggagcatt
     1261 gatttcggct tgtaatagct ctacgcggcg ctgtttgaac tccaatgccg ctttcaccgc
     1321 ctttccgaag ttgagattga taagctcctc gacgacacct gccgtggcag ttacgattac
     1381 ctccatcgtg cctaatttga atttcaggcg attgagttct aattgtgact ggtattgtga
     1441 aagcagtgtt tcaagcttat cgagtatttc gtgcggctga aggcggagcc gggccgtttc
     1501 attagcccag ttacggaggg cggccaatgc gcgcctgttc tcaggcgcac tcctgaactc
     1561 gatgacgtct tcccagcgtg tgctgtcatc aagcacagga aaagctttga tcatgatctg
     1621 cactgttggt acgccggggt cagagttcga catcggctgc ggtatccgtt caatgactgg
     1681 tattacagcg gttccatcga cggcccgaaa ccaaagcgcg gcggaccgag tttgagccca
     1741 tgtcgccaat tccagtgaat cagccacttc ccggagtatg gctttttcat ctggctgctc
     1801 cgcttcaaat ttctcgagag agttggatat cgagagaata gtttccgaga gttcatgaac
     1861 tgcggcgcga gatagtgctt ggtactcttc agtaacgttc tcatattctc gatcttcaac
     1921 ctccggctgg cagataacct ctttagtcag cagccagcga agctcttcag ctaatctaga
     1981 atcctccatt tgggaaagtg ctggtgcaag atcataaata gcgattcggt caaataacag
     2041 agcgagtcgc ttcaaccacc cgccaagaac ctcgcccttg ataattccgt tctgcacccg
     2101 aaatgcattt ggagcta
//
LOCUS       DASRKK010000473         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_37608, whole genome shotgun sequence.
ACCESSION   DASRKK010000473 DASRKK010000000
VERSION     DASRKK010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37608"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFC15_11070"
     CDS             <1..259
                     /locus_tag="VFC15_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10741.1"
                     /translation="FYDDRALCRDIRRKLEFWLDPAALHTLWDRKWNQWKHLLGTKIE
                     VEGTFVQSGKYRKRRGEWELVTWETALPSRLEVKLPPDFQQ"
     gene            371..772
                     /locus_tag="VFC15_11075"
     CDS             371..772
                     /locus_tag="VFC15_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10742.1"
                     /translation="MCSDLRIPGDFDVAQISWRPDYDPFFYRQLAYRARRIYLFREEY
                     IFDVEKAVVVETPQLGHATYVFAKPRNMDNFLALYIKIKKDDIRRNRNNSAERLGFLG
                     RVIHGANPRVWLKEIRQRAGEGIDFAAAMPE"
     gene            1222..1554
                     /locus_tag="VFC15_11080"
     CDS             1222..1554
                     /locus_tag="VFC15_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10743.1"
                     /translation="MRGLFISQRCIQELIPIMAGDPKLLEGKTGAVENDPLLTPEQVA
                     KRLNTSLDWVWDHSSRKTPFLPVIRFGNGPGRAGILRYRASKIEEFIAEQERLSTMRR
                     RNSGRKIA"
     gene            1590..2843
                     /locus_tag="VFC15_11085"
     CDS             1590..2843
                     /locus_tag="VFC15_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10744.1"
                     /translation="MGKSHQRGWVVARGKKWYGYYRRTVLDPVTNAPVTDVVTIVLGL
                     KARLTKSEAREELQKEIAQQTGQNVNGRVMKDGSITFGWFVRNRYLPLREANWKPETA
                     KVKKIQIQRDLVEKFESVPMDAIDKFMLQTHINRLARFCSTDRVLQARAYLKSIFSEA
                     VEQDFLLKDPSHKVTAPLNLRKKDKTVLTWDQLRLVLATLTHRDRVLLTLEMTEALRP
                     SELFALRWRSFEGSKLTITETGYKGKIRPWGKTRRSLGDVHLPAGLANELWRWKQECP
                     DPSPDAFVFPNSEGGFMDTGNYRNRVLAPLAEKLGLPKLNFQVLRRTTATLAQKKGSV
                     KDIQAHLRHAKADTTANEYMQELPESVRQMVDSVYGELKVGRKRAAGSVYLLPNATKR
                     ENRAAARSDLLPNATKRKRGGSASD"
     gene            complement(2849..2925)
                     /locus_tag="VFC15_11090"
     tRNA            complement(2849..2925)
                     /locus_tag="VFC15_11090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2889..2891),aa:Val,seq:gac)
     gene            complement(2990..>3456)
                     /locus_tag="VFC15_11095"
     CDS             complement(2990..>3456)
                     /locus_tag="VFC15_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM10745.1"
                     /translation="VSQTAFRGKLRDHGKTRKSLRTVHLPEGLANDLWLWKQGCPDAS
                     SGAFIFPNSRKRKGAKQNGFIGTDNYRARVLKKLAEELGLSKLNFQVLRRTTATLAQT
                     KGNVKDVQGILGHTKADTTVNVYMQPIEEGVKQTLDAIYAELTAGPKLVAVA"
BASE COUNT          809 a          945 c          994 g          708 t
ORIGIN      
        1 tttctacgat gaccgggcac tctgccgcga cattcgtcgc aagctggaat tctggctcga
       61 tccagccgcc ctgcatacac tctgggaccg caagtggaat cagtggaagc atctgttagg
      121 aaccaagatc gaggtcgagg gcacgttcgt acagagcggc aaataccgca aacgccgcgg
      181 ggagtgggaa ctcgttacct gggagactgc attgccgagc cggctggagg tcaaactgcc
      241 acccgatttc cagcagtaaa tcgaatctgc caaaactgcc tatcatcgct tcggccagta
      301 ttcgcgggct ctggaccaga tccgactttg ccttgaaagt cgggcagtcg agaaagcaga
      361 actgcaacga atgtgttccg atctgcgtat tcccggtgat tttgatgtcg cccagatcag
      421 ctggcgccct gactacgatc cgttcttcta tcgccagctc gcataccggg cacgccggat
      481 ctatctattt cgggaggaat acatcttcga cgtggagaaa gctgtagtag tcgaaacccc
      541 tcagcttggc cacgcgactt acgtctttgc caagcctcgg aatatggata actttctcgc
      601 cctgtacatc aagatcaaga aggacgacat ccggcgaaac cgcaacaatt ccgccgaacg
      661 gctcggcttt ctggggcgcg tgattcacgg cgccaatcca cgagtatggc ttaaggaaat
      721 caggcaacgc gcaggggagg ggatcgattt tgccgcggcc atgccggagt gaatcacgga
      781 ccaatgcgcg cacactttta tggcattcga tttggcggca aattcctcca caccagctga
      841 tttgtccagt caaagtgtaa tcggtgaaat aaatctatta acgatcatga ttctggcgcg
      901 aggcccgtct ttagcagtcg gagcattgga gcgctgcggt gacaatggtt tcagcaagat
      961 aattctgcca ggtacagcca gttagaagcg agtccggacg gtagccccat cgatcggggc
     1021 acaatatccc gatcagtctg tttgactgcc aatcgattta cagctaacaa gaaccgtgga
     1081 gacgccgttt ctagctgccg cacgcgttga ccgggaacgg tccacgcacg agtggagcgg
     1141 cctaccggag aacatgatca gtcgtaaccg ccgtgggaga agcccgggcc tcacccgttc
     1201 gttgctgcgt ggtccgagaa catgcgcgga ctttttatct cacaacgttg cattcaggag
     1261 ttgataccaa ttatggctgg agatcctaag ttactggaag gcaagactgg cgcggttgaa
     1321 aacgatccat tgctcacgcc tgagcaagtc gccaagcggc tgaacacgtc gcttgattgg
     1381 gtttgggatc actcctcaag aaagacgcca tttctgccag tcattcgatt tggcaacgga
     1441 cctgggcggg caggaatcct gcgctaccgc gcgagcaaga tagaggaatt catcgctgag
     1501 caagagcggc tctcgacaat gcggcgtaga aattccgggc gtaaaatagc ctgacccact
     1561 tgttccccca cagaaaaaca gaggaataga tgggcaagtc gcaccagaga ggttgggtcg
     1621 tcgcccgcgg caagaagtgg tacggatact accgcagaac ggtgctcgac cctgtcacca
     1681 atgcaccagt aactgatgta gtcacgattg ttctcggcct caaggctcgg ttgacgaaat
     1741 ctgaggcgcg agaggaactg cagaaggaga ttgctcaaca gacgggccaa aacgtgaatg
     1801 gtcgggtcat gaaggacggc tcaatcacgt ttggctggtt cgttcgcaac cgttatctcc
     1861 cgctgcgcga agcgaactgg aagcccgaga cggcaaaggt gaagaagatt cagattcaaa
     1921 gagacctggt ggagaagttt gaaagcgtgc ccatggacgc gatcgacaag ttcatgttgc
     1981 agacgcacat caatcgactt gccaggttct gctcgacaga tcgggtgctt caggcccgcg
     2041 cgtatttgaa gtcgatcttc agcgaagctg ttgaacagga cttccttttg aaagacccgt
     2101 cgcacaaggt cacggcaccg ctcaacctcc ggaagaagga caagacggtt ctgacgtggg
     2161 atcaattgcg gctggtcctt gcaactctca cccataggga tcgagtgttg ctgacccttg
     2221 agatgaccga agcactgcgt ccgagcgaat tgttcgcgtt gcgctggagg tcattcgagg
     2281 gcagcaagct aaccatcaca gaaacgggtt acaagggaaa gatacggccg tggggcaaga
     2341 caagacggag tctgggcgac gttcaccttc cggcaggact ggcgaacgag ctctggcgtt
     2401 ggaaacagga atgtcctgac ccctcgcccg atgcattcgt ctttcccaac tcagaaggcg
     2461 ggttcatgga tacggggaat taccgcaacc gcgtcttggc accgctggct gagaaactgg
     2521 gattaccgaa gctgaacttt caggtgctgc gccgcacgac ggcgacgctg gcacagaaga
     2581 aaggttccgt gaaggacatc caggctcatc tgcggcacgc gaaagccgac acgactgcca
     2641 atgagtacat gcaggagttg ccggagagcg tgagacagat ggtcgattct gtgtacgggg
     2701 aactgaaggt gggacggaag cgcgctgcgg gctccgttta tttgctgcca aatgctacca
     2761 aacgtgaaaa tcgagcggcg gcaagaagcg atttgctacc aaatgctacc aaacggaaga
     2821 ggggaggatc tgcaagtgat tgaaaatatg gtaggcacga ggagactcga actcctgacc
     2881 tctaccgtgt caaggtagcg ctctaaccaa ctgagctacg cgcctatcgt ttaaagtcta
     2941 ccacttgcgc gccatcggaa aatacccacc gtaccaaagc gtaccaaatt tatgcgactg
     3001 ctaccagctt cggccccgcc gtcaactccg catagatcgc gtccagcgtc tgcttcacgc
     3061 cctcttcgat cggctgcatg tagacgttga cggtggtatc ggccttcgtg tgccccagga
     3121 tgccttgcac gtccttcacg ttgcccttgg tttgggcgag cgtggcagtt gtgcgccgca
     3181 gcacctggaa gttcagcttg ctgagcccta gctcctcggc cagcttcttc agcacccggg
     3241 cccgatagtt gtcggtgccg atgaagccgt tctgcttcgc ccccttgcgc ttgcgcgaat
     3301 tggggaagat gaaggcacca gacgaagcat cggggcaccc ctgcttccac agccacaggt
     3361 cgttcgccag cccttccggc aggtgcaccg ttcgcagcga cttcctggtc ttgccgtggt
     3421 cgcgcaactt gccccggaag gcagtctggc tgacgg
//
LOCUS       DASRKK010000474         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_819168, whole genome shotgun sequence.
ACCESSION   DASRKK010000474 DASRKK010000000
VERSION     DASRKK010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819168"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1584
                     /locus_tag="VFC15_11100"
     CDS             217..1584
                     /locus_tag="VFC15_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZM10746.1"
                     /translation="MATKELRTRRVRARAMAVVATLAAASLLAACGGGATPSSVTTSS
                     GGGGVTPDDHPKGTLNILVSSAPGSDAGFKAVNQAFQAKYPDVKIEFSSIPNENYPAA
                     RASRLSAGNIDVGLAGPQALPSYVPASNEGDDARLARAGGFVDLTGMSFMKNFTPSVL
                     DVIKFDGKQYTVPTGLSYYTGVFYNKAIFTKYGLAIPTTWAEFVKLCETLKSNGVTPL
                     GIGGKDSWPAGLNMLAAVQGLYPTTQDKAGLAEGLWKQTLKLTDTKPVAVLERTKAMF
                     DYAQQNFAGFSYASIPSGFINGKFAMTADGTWDETTIASAAGADFSFGYFPIPTSDNA
                     QDNAYLGGKVELTLAVPSNSRNQGAALAYLQFFSDPTNYQTFLKLAGFAPSQPNITAS
                     PFLTSIQKYTTTFTPAWDTIWVANSKAGPKASFPFNYPGIAPLGSGTAAQAAEQAQAD
                     WAAGN"
     gene            1928..>2686
                     /locus_tag="VFC15_11105"
     CDS             1928..>2686
                     /locus_tag="VFC15_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZM10747.1"
                     /translation="MLNSFVFGRRSTLNISFALGLSVIVVFGIVPAVAVLVISFTNVR
                     ALPYLPVDWVGIENYLSFFSAAHLGYNVNALWNTFVFAFMVTIFQNVIALLIAVLLNQ
                     RLRGRTFYRAVVFLPTILGVTVIGLIWSLVFNPSAGPAATVWSWVGANSAFFGDPGMA
                     LGLVIFVQIWAGLGIAVVIYLAGLQAISPDLYEISGVDGATAWQKLRWITYPLLAPSI
                     TANMLLCIVGSLQSYQLIYVLTGPNNPATQVLSLA"
BASE COUNT          500 a          845 c          779 g          562 t
ORIGIN      
        1 ggcggcatct cgatcggacg ctccggacct atgtcgagca ctacaaccgc gggcgacccc
       61 atcgcgccct cggcctcgcc cctccacgag ccgaagcgtg ggacccgatg ctggtctcac
      121 cgggtgctga attacgttga cggagcacgg gggtggtgtt agcgtcggcc taccgaaacg
      181 cttcggagcg ggtgatttca cttggaggaa tcaaccatgg caacgaagga gttgcgtacc
      241 cggcgtgtgc gtgcccgtgc aatggcggtg gtcgccactc ttgccgcggc atctctcctc
      301 gctgcgtgcg gaggcggggc cacaccttca tccgtcacaa cttcatccgg cggaggcggg
      361 gtcacacctg acgatcaccc aaagggcaca ttgaacatcc tggtctccag tgcccccggc
      421 tctgacgcgg ggttcaaggc agtgaaccaa gcattccagg cgaaatatcc ggacgtcaaa
      481 atcgaattca gttcgattcc aaacgaaaac tatcccgcgg ccagagcgtc gcggttgagc
      541 gcaggcaata tcgacgtggg cctcgcgggt ccgcaggcgc ttccttcgta cgtcccggcc
      601 agtaatgaag gcgacgacgc tcggctggcc agggcaggtg gtttcgttga cctcacggga
      661 atgtcgttca tgaagaactt cactccctcc gtactcgatg tcatcaagtt cgatggaaag
      721 cagtacacgg ttccgacggg gctgagctac tacaccggcg tcttctacaa caaggcgatc
      781 ttcacaaagt acggtcttgc aattccgacc acatgggccg agttcgtgaa gctttgtgaa
      841 acgctgaaaa gcaacggcgt gacgccgttg ggaatcggtg gaaaggacag ctggccagca
      901 ggtttgaaca tgctagctgc tgtgcagggt ctgtacccga ccacgcagga caaggccggc
      961 ctggctgagg gcctctggaa gcagacgctc aagctgactg atacgaaacc cgtggcggtg
     1021 ctcgaacgga ccaaggcaat gttcgactac gcccagcaga acttcgctgg tttttcttac
     1081 gcctcgatcc cgtcgggctt catcaatggc aagttcgcaa tgaccgccga cggcacgtgg
     1141 gacgaaacaa caatcgcttc cgcggccggg gcggacttca gtttcgggta cttcccgatt
     1201 cccacaagcg acaatgcgca ggacaacgcc tatctcggcg gtaaggtcga actcacactc
     1261 gcggtgccga gtaactccag gaatcagggc gcggctcttg cctacttgca gttcttctcg
     1321 gacccgacta attaccaaac gttcctcaaa ctggcaggat tcgcgccgtc gcagccgaac
     1381 atcaccgcga gtccatttct gacgtcgatc cagaagtaca ccacgacctt cacaccggcg
     1441 tgggatacca tctgggtcgc gaacagcaag gcgggaccga aggccagttt ccccttcaac
     1501 tacccgggga tcgcccccct cggcagtggt accgccgccc aggcggctga acaagctcag
     1561 gcggattggg ctgcgggcaa ctaggctagc gccccgcgcc cgaggcgctt gtgatgggcg
     1621 agccacctct tcgccagcga tcggcacttt ggcgacccct tcggctgctc gcaggatgat
     1681 cttcgcgtgc accgcggtgc gcactcggtc gtgcccacgc gggccgtcgc ccgccaaggc
     1741 ttcaacttcg gactcgctca cagccaacgg aggtgcggag attggccgtg ttcagcatcg
     1801 cagccgcgga catccgaacc gatgatcctc atgtcgagcg ggacactgaa gtgctggggt
     1861 ccgcccggcg acgttcgttg ggccgacctc ctcttcatct aggccattgc agaacggatc
     1921 tttacggatg ttgaactcct ttgttttcgg acgccggtcg actctcaaca tctcattcgc
     1981 gctcggcctg agcgtgatcg ttgtcttcgg aatcgtgccg gcggtggccg tcttggtcat
     2041 ttcgttcaca aacgtgcgcg cgctgccgta tctgcccgtg gactgggtag gcattgagaa
     2101 ctacctctcg ttcttctcgg cagcgcatct tggatacaac gtgaacgcgc tctggaatac
     2161 cttcgtcttc gcgttcatgg tcaccatctt tcagaatgtg attgccctcc tgatcgccgt
     2221 cctgctcaat cagcgcctgc gcggcagaac cttctaccgc gctgttgtct tcctgccgac
     2281 gatactgggt gtcacggtga tcggactcat ctggtcactt gtcttcaacc cctccgcagg
     2341 cccggccgcc accgtctggt cgtgggttgg cgcgaattcg gcgttctttg gtgaccccgg
     2401 catggctctt ggtttggtca tcttcgtgca gatttgggct ggcctcggca ttgccgttgt
     2461 catctacctg gccggactgc aagccatttc tcccgatctg tacgagatct cgggcgtcga
     2521 cggtgcgacc gcttggcaga agcttcggtg gatcacgtac ccgctcctgg ctccatcgat
     2581 cacggcaaac atgctgctct gcattgtggg ctcactccag agctaccaat tgatatacgt
     2641 gctcaccggc ccgaacaacc ccgcgactca ggtgctctct ttggcc
//
LOCUS       DASRKK010000475         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1406631, whole genome shotgun sequence.
ACCESSION   DASRKK010000475 DASRKK010000000
VERSION     DASRKK010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406631"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFC15_11110"
     CDS             <1..305
                     /locus_tag="VFC15_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM10748.1"
                     /translation="TISTEELVSQMNAALKLPGVSNSWTMPIRGRIDMLTTGIRTPVG
                     LKIAGADLEHIQEIGAQVESALKTVKGTRTVLAEHTADGYFLDLQWDREQLARYGT"
     gene            441..1895
                     /locus_tag="VFC15_11115"
     CDS             441..1895
                     /locus_tag="VFC15_11115"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM10749.1"
                     /translation="MVTDRNQDSDKTPVKQGPGKVSSEPNKINASTPYDFSGKNLTPY
                     GGLLPVITMLENLGFQTLVEQTVTSKRISRAMDLYRFVLAIVLTLYIGFPRLNQLRFI
                     ARDPILTGILKVGKLPVQSTFWRFVNALHLNVARQILAIMRIMRARVWEAANVKLAVV
                     TIDTDTTVHTLYGQQMGGRKGYNPKNKGKKSYQPMLTFIAETREYVWGELRNGDRPTG
                     KQIGDHIRSVCAALPSCVKRIFGRADSGFYCREAVEAYEECNARFAISARKTSRLVEK
                     LRQAEWKPSNKTDAGWECEFRYQPDGWKKEYRFVALRYEKTREEQEAAEKEQYQLFET
                     SQYKYRVFVTDMPDPIYFVVWFYNQRGGAENLIKEANNDAGLAAHPSSRFDVNGNHFQ
                     LAMLAYNLNCWLMLFNREPQADADAMRHTTLATSRLRFLFVAAKIWRHAGRTGVSYSD
                     SYEERGVFERLMDRLRRIAPRGSGFAAVMQPALR"
BASE COUNT          537 a          621 c          702 g          441 t
ORIGIN      
        1 atacgatctc gaccgaggag ttggtcagcc agatgaacgc agcgctgaaa ctccctggtg
       61 tcagcaactc gtggacgatg ccaattcgag ggcgcatcga tatgttgacc accgggatca
      121 ggacgcctgt ggggctgaag atcgccggcg cggatttgga gcacattcaa gaaatcggcg
      181 ctcaagtcga gtcggcgctg aagactgtaa aaggaacgcg cacggtgctt gcggagcaca
      241 cggccgacgg ctactttctt gatttacagt gggaccgcga acaactggcg cgttatggta
      301 cctgagtcat gcgtgggctt gagcccgcgc gtgtcagcaa agccgtaggg aaccatttgg
      361 ggaccccgat attattgggg taaaagagaa gttctgtgaa gagtgcagct tcgcagaccg
      421 accccaagga ggtcacccaa atggttacag acagaaatca ggatagcgac aaaacccccg
      481 taaaacaagg tccggggaaa gtctcgtcgg agcccaataa aatcaatgct tccacgccct
      541 atgacttctc cggtaagaat ctgactccct acggtgggct gctgccggtg atcaccatgc
      601 tggagaatct cggcttccag acgctggtgg aacagacggt gacttcgaag cgaatttctc
      661 gtgccatgga cttgtaccgg ttcgtgctgg caatcgtgct cactctctac atcggttttc
      721 cacggctgaa tcaattgcgt ttcatcgctc gcgacccgat cctgacgggc atcctcaaag
      781 tcggcaagct gccggtgcaa tcgaccttct ggagattcgt caatgcgctt catctgaacg
      841 ttgcccggca gattctggcg atcatgcgaa tcatgcgcgc aagggtctgg gaggcggcca
      901 atgtgaaact ggcggtcgtc accattgata ccgacaccac ggtgcacacg ctgtacggcc
      961 agcagatggg cggacgcaaa ggctacaacc cgaagaacaa aggcaagaag agctaccagc
     1021 cgatgctgac gtttatcgcc gagacgcggg aatacgtctg gggcgaactg cgcaacggtg
     1081 accgtcccac gggcaaacag atcggcgatc acatacgaag cgtgtgtgcg gccctgccgt
     1141 cgtgcgtgaa gcggatcttc gggcgggccg attcggggtt ctactgcagg gaagcggttg
     1201 aggcttacga agaatgcaac gcccggttcg cgatctcagc gcgcaagacg tcccggctgg
     1261 tcgagaaact gcgccaagcc gagtggaagc cgtcgaacaa gaccgatgcc gggtgggaat
     1321 gtgaattccg ctatcaaccg gatgggtgga agaaggaata ccggttcgta gctctgcgct
     1381 atgagaaaac gcgcgaggag caggaggcgg cggagaaaga gcaataccag ttgtttgaaa
     1441 ccagccagta caagtatcga gtcttcgtga ccgacatgcc cgatcccatc tacttcgtgg
     1501 tctggtttta caaccaacgg ggcggcgcgg aaaatctgat caaagaagcg aacaacgatg
     1561 ccgggctggc agcgcaccct tcgagtcgct tcgacgtgaa tggtaatcac ttccagttgg
     1621 cgatgctggc ctacaacctg aactgttggc tgatgctatt caaccgcgag ccgcaggccg
     1681 acgccgacgc aatgcgccac acgactttgg ccacctcgcg gttacgattt ctgttcgtgg
     1741 ctgccaagat ctggcgtcac gctggccgca ctggagtgag ctacagcgat agctacgagg
     1801 agaggggtgt gttcgagcgg ctgatggatc gactgcgccg gatcgcgccg cgtggatcag
     1861 gcttcgcggc ggtgatgcag ccagccctgc gctgaaaacc ttctccgccg gcctttcggt
     1921 ggctgtgcat agaagtttat gcacggactg caaaagcaac ggagcgagtc acttcggcgc
     1981 aaggcttggc ggaaaagaac tctggggtag gaggtgaagc agcgaaaact aaactgactt
     2041 actcacacac gggtggcatc agccgagtgg gggcatcgct cggaagggtg gttgtgtgca
     2101 taatccaggt atcaggcaca tcgcctgtcc ggagaagagt cgtgggagca gggtactcgt
     2161 ccggtccggg cctatctact tcgcttctgc cgtgaaaagg accttgctct catactcgaa
     2221 gtcacgaaaa cgccaccgcc caaaaaaccc aatgaactcg gcctgaggat tctcgccccg
     2281 gcctacgtgc tgtcagaact a
//
LOCUS       DASRKK010000476         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_38145, whole genome shotgun sequence.
ACCESSION   DASRKK010000476 DASRKK010000000
VERSION     DASRKK010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38145"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..671
                     /locus_tag="VFC15_11120"
     CDS             333..671
                     /locus_tag="VFC15_11120"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM10750.1"
                     /translation="MAQTTKVDRLLARLPSLADIVRGSLLHRTTFHSGGCSKCARGIG
                     HPQWVLNVNYPGGKNRQISLHRNQLLQVRRWLKNYHQLKKALEAICELNQRSLRAKSS
                     QRATRSNGHD"
     gene            664..2073
                     /locus_tag="VFC15_11125"
     CDS             664..2073
                     /locus_tag="VFC15_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057901383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HZM10751.1"
                     /translation="MIKARSRPRGLAQGFLAEPMDNMWQDWMRQVDQVLDDEQLVDLV
                     YEALVRRHPRSRTRGRFGTPAEVVLRMLVLKHLRNWSFQILEWEVRANLVYRQFTRVG
                     GNKVPDAKTLGRQALALGPEVIQKIHHRVVGIAREKQIATGRKLRVDTTVVETNIHYP
                     TDSSLLGDGVRVLTRVMKRVTEVAGEVGTKLRDRSRSVKLRAVEIARVSRSKSEQGQQ
                     RMKDLYGKMLDSSGRVAGQAQRFANEIASGVKRSADIKKQAALEGMKKELDTMVGRVK
                     QVRLQARARVFGGNTHVAGKLASMFEHTTEIIRRGKAKQPTEFGKMIKVQEAEGQLIV
                     DYEVFDKRPADSDLLVPAIAVHQQRLGRVPDLVAGDAAFYSARGEKEAHEMGVKRVSV
                     PNRSSKSKDRTALQKTRWFKNGQRWRTGCEGRISVLKRRHGLSRCRYKGDRGMQRWVG
                     LGVISDDLINIGRALAAKT"
BASE COUNT          550 a          704 c          749 g          447 t
ORIGIN      
        1 ggccaaaact aaaatggtcc cgttcatttc agcccgcgac aaactgccgt ttgaaatcac
       61 gcctttcatg atcttcctga cccgcgccgg ctcggctcca aaggagcagg gtccggaccc
      121 aagcagcagt cggcttgagg atcgttagct actttccggc gcaaaaatga gggtctaagt
      181 cccgtgattt catagggcgg gcagtcggcc ccgtgcgggt tcgaacaagt tcgaccggtc
      241 tcggtgcctc tcggcgtccc ctcgtcaagc tttaaacatt tcctacagaa atgtttttac
      301 atcgtggagg catctgtgta tcatctatat agatggcaca aacgaccaag gtcgaccgcc
      361 ttctcgcccg gctaccttcc cttgcggata tcgtgcgcgg ctccctcctc catcgcacta
      421 ccttccattc cggcggctgc tccaagtgtg cccgcggcat cggtcatccc cagtgggtcc
      481 tcaacgtcaa ctatcccggc ggcaagaacc gtcagatcag cttgcaccga aaccagttgc
      541 tccaggtccg gcgctggctc aaaaactacc accaactgaa gaaggcactg gaggccatct
      601 gcgagctgaa ccagcgctcc ttgcgcgcga aatcctcgca gcgcgccacc cgcagcaacg
      661 gccatgatta aggcgcgcag ccggccacgt ggcttggccc aaggtttcct cgccgagcct
      721 atggacaata tgtggcaaga ctggatgcgc caggtggacc aagttctgga cgatgaacaa
      781 ctggtagatc tggtgtatga agccctggtg cgccgtcatc caaggagtcg cacccgcggg
      841 cggtttggta cgcccgcgga agtcgtcttg cgcatgctgg tactgaagca cctgcgtaac
      901 tggagcttcc agattctgga gtgggaagtg cgggccaatc tggtgtaccg ccaatttacc
      961 cgagtgggtg gaaacaaggt gcccgatgcc aagacgctgg gacgacaagc cctggctttg
     1021 gggccggagg tcatacagaa aattcatcac cgcgtggtgg gcatcgcgcg tgaaaagcag
     1081 atcgccactg gccgcaagtt gcgcgtggac acgacggtag tggaaaccaa cattcattat
     1141 ccgaccgata gcagcttgct gggcgatggc gtccgcgtgc tgacgcgcgt catgaagcgg
     1201 gttaccgaag tggccggcga agtgggaacg aagttgcgcg atcgctcacg gagtgtgaaa
     1261 ctgcgggccg tagaaattgc gcgcgtcagc cgcagcaaga gcgaacaggg ccagcagcgg
     1321 atgaaggatc tgtacggcaa gatgctggac agtagcgggc gggtggccgg tcaagcgcag
     1381 cgcttcgcca acgagattgc ctctggcgtc aaacgtagtg cggatatcaa aaagcaagcc
     1441 gcgctggaag gtatgaagaa ggaactggat accatggtcg gccgggtgaa gcaagtgcgg
     1501 cttcaagcgc gggcgagggt gttcggcggc aatacgcatg tagcaggaaa actggccagc
     1561 atgtttgagc acaccaccga aatcatccgg cgcggcaagg cgaagcagcc gaccgaattt
     1621 ggcaagatga tcaaggtgca ggaagcggag gggcaactca tcgttgatta cgaagtcttc
     1681 gacaagcgcc ccgctgacag tgatctgctg gtccccgcga ttgcggttca ccagcaacgg
     1741 ctgggacgag ttcccgactt ggtggccggt gacgcagcgt tctattccgc ccgcggcgag
     1801 aaggaggcgc acgagatggg agtgaaacgc gtttccgtgc ccaaccgctc gagtaagagt
     1861 aaagatcgca cagcgctcca gaaaacacgc tggttcaaga acggacagag atggaggacc
     1921 ggctgcgaag gccgcatcag cgtgctcaag cgacgccacg ggctgagtcg ttgtcgctac
     1981 aaaggagacc gcggaatgca gcgatgggtg ggtttgggcg tcatcagcga cgacctcatc
     2041 aatatcggcc gcgccctggc agccaagacc tgaagccata tccgcccggt tgtcccttcc
     2101 gtcctcgcga ttcgaccctg gggcgccatt tctcttggcg gccatctctg actgcggcca
     2161 atctgccatc tgtatcgatg gcacattctc tcaaccatat cccgtagcaa accgcttgac
     2221 ctggccggca ggcatggcaa cttgtaggga ccagctgaaa ggcggtccgg ccagcaatga
     2281 aaacacaggg gatagaccct catttttgcg ccagaaagta gctagcacta cacgaggagc
     2341 agccggatca acaaaccggg cgatagagag cgggttcttg cggacccaga cccttgaatc
     2401 tgcactgatc gggataggaa ctcagtggcg gccctgacgg gcgccgaacg
//
LOCUS       DASRKK010000477         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_722724, whole genome shotgun sequence.
ACCESSION   DASRKK010000477 DASRKK010000000
VERSION     DASRKK010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722724"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFC15_11130"
     CDS             <1..185
                     /locus_tag="VFC15_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10752.1"
                     /translation="NSQLSPNFIQGSGALDRFGSLDFDGQPTSTTTTTRVSNVRIGMP
                     ESIIQSSRVDCASRAT"
     gene            complement(511..1887)
                     /locus_tag="VFC15_11135"
     CDS             complement(511..1887)
                     /locus_tag="VFC15_11135"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZM10753.1"
                     /translation="MILSDVFERYVKKSPVSVMARTMMEMALAREDLDGLFEQHTDRQ
                     YTRSLLFSTIVDLMGVVVSKSQPSIHAAFQEVRETLPVSLTAVYDKLNGLESTVTSAL
                     VRHSADKLASVIVAMKGQLPPLLPGYRARIIDGNHLAATQRRIKVLKRSKAGPLPGHS
                     LVVLDPELMLATNMIPCEDGHAQERSLSSEILKLVRERDVWIADRNFCTTALLFGIAT
                     RDAFFVIRHHANMTLEPLSPLKKHGQTETGEVSEQMVAVTNSDGEVLKMRRVAIRINA
                     PTRDGDQEMAILTNLPKGAADATTVSEVYRKRWTVETMFQSLTQMLQGEIDTLGYPRA
                     ALLGFGVALTTYNVLSTVQAALRAKFGVEKIQQEVSAFYIAHEVQASHPGMEIVFDPE
                     VWDPYSKMSPKALAKEMLVWASHVKLSKYQRHPRGPKKPVPKRNRFVDKMHVSTARLL
                     AKSRKKSP"
     gene            2210..3217
                     /locus_tag="VFC15_11140"
     CDS             2210..3217
                     /locus_tag="VFC15_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10754.1"
                     /translation="MTTVRFASIDVFRKLSVYLTGTAAAVSLLLASELAAAATCVSID
                     ESRDNLTPADREGARALFEEALRDNSVQVARENCTETWVLYHIKLGSSVTVIVHSPRG
                     DRKEQVTGLSELSGMYSQIARSIQTGIVGSSESPIVDRKNVTDNQTMPNRLPADSIWY
                     LTLGYGATVASGLYGGPSFGIGKRWELDRVGIDLSFLNVAIFQGQNGTQGAAGAWLKI
                     GAEYFFDAYSNYSPYIGAGLSLSSNNIDTPTTRFSGWGANAELTAGYELFRASTIRML
                     LQANVSLPTYTLSGEPVYSYNAGVATSNVAANRYAPVFGLSLGIGWGKPREVAVVKVK
                     E"
     gene            complement(3749..>3938)
                     /locus_tag="VFC15_11145"
     CDS             complement(3749..>3938)
                     /locus_tag="VFC15_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10755.1"
                     /translation="LDRAWDDVAVEMTSSAYRESLPTPYDADPLRFDVVQSEHAGKHR
                     DKLATLPEVDPLRFDVVP"
BASE COUNT          855 a         1066 c         1086 g          931 t
ORIGIN      
        1 aaaactcgca gttgtccccg aacttcatac agggaagcgg agcgcttgac cgcttcggat
       61 cgttagactt tgacggacag cccacaagta caacaaccac gaccagagtg agcaacgtac
      121 gcatcggcat gccggagtct atcatccaat cgagccgcgt cgattgcgct tcgcgagcga
      181 cctagaactt tgttatccaa gtagcggggg ctgcgctcgc caatcgctga tcttaatttg
      241 ttggttgctg cggcacggaa tcggctgttt caggctcggg acctgttaca ggaccaggac
      301 ctgttacaga accaggacct gttacagaac cagggaccgt cacagaacca ggacctgtta
      361 caggaccagg taaccccctg tctcgctccg agccgatcaa cttgagattc cagcacgatt
      421 cctgcggaaa tcgtcagtct cgttgatttc aacgtggtga tcgtgcttct cttcgctttc
      481 tctgcccctt gaatcgaaca accagccctt tcaaggtgac ttctttcgtg attttgcaag
      541 aagtctcgcg gtggacacat gcatcttgtc tacgaatcga tttcgtttcg gtaccggctt
      601 cttcggtccg cgggggtgac gttggtactt actcagcttg acgtgcgacg cccacaccag
      661 catttctttt gctagtgctt tgggcgacat cttcgaatat gggtcccaga cttctgggtc
      721 aaagacgatt tccattcctg ggtgcgatgc ctgcacttcg tgcgcaatgt agaaggcgga
      781 cacttcttgc tggatcttct cgactccaaa ctttgcgcgt agcgccgctt gtaccgtgga
      841 aaggacgttg taggtagtga gcgcaacacc gaatccaagc agcgcagctc tggggtagcc
      901 tagcgtgtcg atttcgcctt gcaacatctg ggtgagcgat tgaaacatcg tttcgaccgt
      961 ccatctctta cgatacactt cggacactgt tgttgcgtcg gcggcaccct tgggcaaatt
     1021 ggtcaatatg gccatctctt ggtcgccgtc tcgagtcggg gcattgatcc gaattgcgac
     1081 ccgtctcatc ttgagcactt cgccgtcgct attcgtcact gccaccatct gttcgctgac
     1141 ctcgcccgtc tccgtttggc cgtgcttctt cagtggcgag aggggttcca acgtcatgtt
     1201 ggcgtgatgc cgaatcacga agaacgcgtc acgggtggcg ataccaaata gcaaggcggt
     1261 ggtgcagaag ttgcggtcgg caatccaaac gtcgcgttct cttaccaact tgaggatttc
     1321 agacgacaat gaccgctcct gagcgtgtcc gtcttcgcag gggatcatgt ttgtggcgag
     1381 catcaactct ggatcaagaa caaccaatga atgcccaggc aacgggcccg ccttgctgcg
     1441 cttgagcacc ttgattcgtc gctgggttgc agccagatga ttgccgtcga taattctcgc
     1501 tcgatagccg ggcagtagtg gcggtagttg gcctttcatc gcaacaataa ccgaggccaa
     1561 cttgtctgcc gaatggcgaa ccaatgcgct ggtcaccgtg gactccagtc cattgagctt
     1621 gtcgtagacc gctgtgagcg acacggggag tgtctccctc acctcttgaa acgctgcatg
     1681 aattgacggc tgactcttgc ttaccaccac tcccatcaaa tcgactattg tcgagaacag
     1741 caggctacgt gtgtattgcc ggtcggtatg ctgttcgaac agaccgtcga gatcctcacg
     1801 cgcgagagcc atctccatca ttgtgcgggc catgacgctc actggcgact tcttgacgta
     1861 gcgctcgaaa acatccgaca aaatcatgcg acactagatg ccggagatgc tggcccatgg
     1921 acagattgat tcgcgctctc accgaaaaca gatcccacaa agtgcgatat agatcagatg
     1981 gacctacatc gcggccacct tgaaagggct ggtgccggca cctgtatatg aatcgccaca
     2041 gtacatgcac attcgcccag ttgcgccctc ccaaaatccc ggcattttag gcaacatccc
     2101 aagctggcac gggtcgtgct taatggagac tcaagctcag cggcatgttg accgagggtc
     2161 tgcggcctgt tcgggtgatg ctatcctctg cgcttaggga gatcagagca tgaccacagt
     2221 tcgattcgct tcaattgatg tattccggaa gctttcggtc tatcttaccg gcaccgccgc
     2281 tgcggtttca ctgctgctcg ctagtgaatt agctgcagcg gctacttgtg tgagtattga
     2341 cgagtcgcgt gataacctca cccccgccga ccgagagggt gctcgtgctc tatttgaaga
     2401 agctttgaga gacaactcgg tgcaagtcgc gcgcgagaac tgcacggaaa cttgggtact
     2461 ctatcacatc aagttgggaa gcagtgtgac cgtaatcgtg cacagccccc gtggcgaccg
     2521 caaggaacaa gtgacgggtt tgagtgagct atcgggtatg tatagtcaaa tcgcgcgttc
     2581 gattcaaacg gggattgttg gttcgagcga aagcccaatt gtagatcgaa agaatgtgac
     2641 ggacaaccag accatgccga atcgcctgcc ggcagactcg atctggtact tgacgttagg
     2701 ctacggcgcc acggtggcgt cggggcttta cggcggaccg tcatttggca ttggcaaacg
     2761 ctgggaactc gatcgtgtgg gcattgactt gtctttctta aatgtcgcta tcttccaagg
     2821 gcagaacgga actcaaggcg ctgcgggagc ctggctaaaa ataggtgcag aatacttttt
     2881 cgacgcctat tcgaactatt caccctacat tggcgctggg ttgagtctct cgagcaacaa
     2941 tattgacacc ccaactacgc gcttctcagg ttggggtgcc aacgccgagc ttactgcggg
     3001 ttacgagttg tttcgcgcca gtacgattcg catgctcctg caagccaacg tcagtctgcc
     3061 cacttatacg ttatccggcg aacccgttta tagctacaat gcgggtgttg cgactagcaa
     3121 cgttgccgca aatcggtatg ccccggtatt tggccttagt cttggtattg gttggggcaa
     3181 gccgcgcgaa gtcgcagtcg ttaaggtcaa ggaatagcga ctcacttgaa tacctggaga
     3241 acaatagcat tcatagtggg tttgcccctt gctgcacttg gatgcagcaa aagcgaccac
     3301 tgcaatcctc caccaaggtg ggatcaccaa tgcgctgacg cagctattct ttggaatgag
     3361 tactacagcc ataagtatcc gggtgggcgt acacaggacg cggcggcgga ggttggcata
     3421 ttggacgcag ggggaattaa ttgccccacg gcaaagcagc tgcagtatga agatgtgagc
     3481 ccttactcag ctgatctaat gggagatggt ttcgtatcgg gtcctgaaac tggacgcgcg
     3541 gacaatttgt gctgttatca aacaggtgtg ctttgctatt aatgaagtat ccgggatctc
     3601 cgtttttgga ccggatccgc cgggcctgct ccagttgatc gaagcggaga aagctgtctc
     3661 gatgccgccg cgcacctgtg cccaaggttt ggagcgtttg gcgagcaccg tgagttaagg
     3721 agcgacgcga cagcctcgct gtttcggctt acggaacgac gtcgaagcgc agaggatcca
     3781 cttccggaag cgtcgccagt ttgtctcgat gcttacccgc gtgctcggac tgcacgacgt
     3841 caaaacggag tggatcggca tcataggggg taggcaagct ttcacggtac gccgatgacg
     3901 tcatctcaac ggccacgtca tcccaggcgc gatccaat
//
LOCUS       DASRKK010000478         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1015990, whole genome shotgun sequence.
ACCESSION   DASRKK010000478 DASRKK010000000
VERSION     DASRKK010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015990"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..482
                     /locus_tag="VFC15_11150"
     CDS             282..482
                     /locus_tag="VFC15_11150"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZM10756.1"
                     /translation="MTSTTVVKDPVCGMDIETATAAGQTEYKGQTYYFCGSKCKENFD
                     LKPDQYLSKSAGTAKSGPGCCG"
     gene            1161..2648
                     /locus_tag="VFC15_11155"
     CDS             1161..2648
                     /locus_tag="VFC15_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10757.1"
                     /translation="MSIQRQEGTAVYISVNQTKLLLPRHFAANVAVGDEITFPIPAPD
                     AVGPEILVTKNAASGPRRYLYQAPIGYVSQPKQDKRNQYFVSAEVRGGSLGISAVFLP
                     CAVLCEYFYRLPRAADRVDNPFLYEVLRIPASASPSELRVAFKLRDLELRTAGVRHRE
                     RVALERAFNIVGQPELRACYDGLLADAEAPAIFPYGGFGSLLVAGEPSRDGQTFFARR
                     ILAFSPELRRRRFHVPLRRCDFYDERALCRDARRKLEFWLDPAVLHTLWDRTWNQWKH
                     VLGTKIEVEGTFVRSGKYRKRREQWELMTWETALPSRLEVKLPPDFQQQLETAKTTYH
                     RFGQYSRALDQIRLCLQHRAVERAELQRMCSEMRIPGDFDVTQISWRPDYDPFFYLQL
                     SRRARRIYLFRDEYIFDVEKAVVVETPQVGHATYVFAKPRDLNTFLALYTKITKDDIR
                     RNRNNAAERLGFLGRVIHGTNPRAWLKEVRQRVGEGIDVAAAMAE"
     gene            3120..3365
                     /locus_tag="VFC15_11160"
     CDS             3120..3365
                     /locus_tag="VFC15_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM10758.1"
                     /translation="MFSQPSLASSQSDFDREPFVAADRAAAYLGVTRRTLLQKARLGK
                     IPAYPLDPGAKKKEWRFKLSELDRHVCASVNSCLQPT"
BASE COUNT          726 a          933 c          986 g          729 t
ORIGIN      
        1 gttttgcact tctgcaaggc ggccgtgcga agtcgtgagg agtagcgtcc ggaattcggc
       61 gtagttccgc ggtctgaggg tacatgcgat tggccgggcg acggcggtca ctcttgctca
      121 gaagtaacgg caccacgatt gctaacctct gtaccagtgc gctgcactgg cgttttccag
      181 acgacgaaga ggcagtccag tgcgggatga gtttctgaaa ccggccaatc aacatttggc
      241 ctgccggcga gcgaaaagtt acgaacgaag gagagatcaa aatgacgagt acaacagttg
      301 tgaaagatcc tgtgtgcggt atggacatag agacggctac cgccgctggt caaaccgagt
      361 acaaagggca gacgtattac ttctgtggct ccaagtgcaa agaaaacttc gatcttaagc
      421 cggatcaata cctgagcaag tcggcgggaa cggcgaagag cggccctggc tgctgcggct
      481 aaattcaaca accccgcagg tgtaagtcat aagaactggt ttctttctgc cgtcttggac
      541 cgcggtgttg tcgggtagcc agctttacgg cggccattct cagaaccagt gccctgacgg
      601 cggcgtttgg tttgattgag ccgatcgtta tcggtaatcg atttcagaac aaatgggaga
      661 tgaagggatg atggacgggc agaagaagtg agtctgacag cgtttcatat tcccaaacag
      721 cagttaagga ggaggtcccg tgcacggaaa cggcatgcaa agttggaata tgggtggcaa
      781 tgtggtttgg atttgtttgt ttggagtggt gctgatcatc gctctcgccc tgatattgcg
      841 gaggcaaagg tgaacttctg tgcgctcacc acgtggaaaa ccagacaatt tcgcgcaaag
      901 actttcccgc aacacgcgca cccgatgaag tgagcatcca cgctgtggcg ggatcgattc
      961 ccaatcgccg agccgaggct gcagcgaaac cagcgttatg aggcctatcg cacggcgagt
     1021 ggtggggcag tttccggatc gacggcgact ccagtccgaa acagcgtttt gcacttctgc
     1081 aaggcggccc tatactgcca gccgacagca tggctcttcc gtccttcagc ccgtcgactg
     1141 cggtatgccg cgaacgtgtt gtgagcatac agaggcaaga gggaaccgct gtttacattt
     1201 ccgtcaatca gaccaagctt ttgttgcctc ggcacttcgc agccaacgtc gcggtcggtg
     1261 acgaaatcac gtttccgatt ccagcaccag atgcagtcgg cccggaaatc ctcgtcacca
     1321 agaatgccgc ctccggcccc cgtcgttatc tctatcaggc gcccatcggt tacgtcagcc
     1381 agcccaaaca ggacaaacga aatcagtact tcgtatcggc cgaagttcgc ggaggaagcc
     1441 tcggcatcag cgcggttttt ctgccatgcg cggtcctgtg cgagtacttc tatcgcctgc
     1501 cgcgtgcggc cgacagggtc gataatccct ttctgtacga ggtcttgcgt atcccagcca
     1561 gtgcttcgcc ctccgagcta cgcgtagcgt tcaagctgcg tgatctggag ctgagaacgg
     1621 caggcgtacg gcaccgcgaa cgagtggcgc tcgaacgggc attcaacatc gttggtcagc
     1681 cggagctgcg cgcttgctat gacgggctgt tagccgatgc cgaggccccg gctatatttc
     1741 cttatggtgg gttcggctct cttctcgttg ccggcgagcc ttcccgagac ggacaaacat
     1801 tctttgcccg ccgcatccta gcgttttcac cggaactgcg gcgcaggcgg ttccatgtac
     1861 cgctgcgtag atgtgatttt tacgacgaac gggcactgtg ccgagacgcg cgccgcaagt
     1921 tggagttctg gctcgatccg gccgtcctgc atacactctg ggaccgcacc tggaatcagt
     1981 ggaagcatgt gttaggaacc aaaatcgagg tcgagggcac gttcgtacga agcggtaagt
     2041 atcgcaaacg tcgtgagcag tgggagctca tgacctggga aaccgctttg ccgagccggc
     2101 tggaagtcaa actgccgccc gatttccagc agcaattgga gacggccaaa actacctatc
     2161 atcgcttcgg ccagtactca cgggctctgg atcagatccg gctgtgcttg cagcatcggg
     2221 cggtcgagcg cgcggagctg cagcgaatgt gttccgagat gcgcattcca ggcgactttg
     2281 atgtcaccca gatcagctgg cgccctgatt atgacccgtt cttctatctt caactctcac
     2341 gtcgggcacg ccggatctat ctgtttcggg atgaatacat tttcgatgtt gagaaagcgg
     2401 tggtcgtcga aacccctcag gttggccacg caacttacgt cttcgccaaa ccccgggacc
     2461 tgaatacctt tctcgccctc tataccaaga tcacgaagga cgatatccgg cgaaaccgca
     2521 ataatgcagc ggagcggttg ggctttctgg ggcgcgtgat tcacggcacc aacccaagag
     2581 cgtggctcaa ggaagtcagg cagcgggtag gggagggaat tgacgttgca gcggccatgg
     2641 cggagtgaag cgcggaccaa ttcgcgccca cttttgtggc atttaatctg gcggttaact
     2701 cctgcgcaaa agctgctttg cccagccaaa gtgtaatcgg tgaaataaag ttagtcgccc
     2761 gtatgtggac tggaattccg ctcgtcggac gatgatgacg gcggagctgt ttccttcttc
     2821 ggatgcggcc cacgatgtcg ggggggaatc ccggcactcg cagcacgaac ctggcaatcg
     2881 tcttgccatt caagtagcgg ttgcgcgcgc catgatggcg acaaatcgtg cggatgcggc
     2941 gcgaaaacca cgcgccgcag tcattctcgg cagaatccga gtcactgaat cctgcaggga
     3001 gcgagtcgct gcgttgctca gtcgaaacct gccatggact ctgatttgac gaattaactt
     3061 tttgggctag aaatgcactt cacggggcct tcgttctgac cccaactttg gagttgatta
     3121 tgttcagcca accctcccta gcgtcgtccc aatcggactt cgaccgtgaa ccatttgttg
     3181 ccgccgacag ggctgccgca tatctcggtg tgactcgccg cacgttgttg caaaaagccc
     3241 ggcttggcaa gatcccggct tacccgctgg atcccggcgc caagaagaaa gagtggcgat
     3301 tcaaactctc ggagctagac cggcacgtat gcgcttcggt aaactcttgc ctgcagccca
     3361 cctgagccat cgca
//
LOCUS       DASRKK010000479         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_232982, whole genome shotgun sequence.
ACCESSION   DASRKK010000479 DASRKK010000000
VERSION     DASRKK010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232982"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1001..1585)
                     /gene="kdpC"
                     /locus_tag="VFC15_11165"
     CDS             complement(1001..1585)
                     /gene="kdpC"
                     /locus_tag="VFC15_11165"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZM10759.1"
                     /translation="MKQNLKIAILMTIVMTVLLGLVYPLVVTALAQLMFRDKANGQLV
                     SRNEEIVGSRIIGQAFVGPGYLHSRLSAAGSGNGYDAANSGGSNLGPTNQHLIDRVKA
                     DVARLQAENPGQPVPVDLVTTSASGLDPDVSPAGAEFQVPRIARERGLSEAQVRQIID
                     QHTSGRQIGFLGEPRVNVLEVNLALDQLKSMARK"
     gene            complement(1597..>2230)
                     /locus_tag="VFC15_11170"
     CDS             complement(1597..>2230)
                     /locus_tag="VFC15_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZM10760.1"
                     /translation="DNPLTAAAIAREAGVDDFLAEATPKEKMDLIRREQAEGKLVAMT
                     GDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEVVAIGKQLLMTRGS
                     LTTFSIANDVAKYFAIIPAMFAGTFPVLNALNIMHLRTPGSAILSAVIFNAVIIIALI
                     PLALRGVKYRAMGAAALLRRNLWVYGVGGIIVPFIGIKLIDMFITGVGLA"
BASE COUNT          463 a          665 c          611 g          491 t
ORIGIN      
        1 tcggcagagg cacccctcgt gcaactgcct actctccgct gctagggctc ccgttctcac
       61 cgattctcgc tgcttttcat tttcctgaag cctgcttcct gctgctcgat tagagaggta
      121 agggatgaaa cccatattgg cttttaccaa gaggtcagtc ccttcgggtt ggctgactgc
      181 tcttgctacc gaagttatta gccagtccat attgatcctt gcaatgatag tcattctcgg
      241 cgctgtctta ctgaccgcac gaggtgttga atggcttttt cctcatactg ctagggcggt
      301 tctgaaggtc ctgcactgta agcgtcagag gaagcacacg gttgacggat cgagtccgca
      361 gccagcgtca aaacgcggtg gtggtccaag ggaacccagg ccccggttaa tgtcgcgcga
      421 cttcaccact tcaccagcaa attcgcaggc gacacaaccc ctgatttcgg caattatccc
      481 catccagcgg tttgtaatga ccagctttgg ttccagaagg attacgtcct gtttgtctcc
      541 gtgatacgcc cacaatcttg gccacagaac gtctttagca ggctcaagcg tggaatctca
      601 ctggcgcaca agccttcgct ttggcagact cttcttctcg attattcggc ggcgcgtccg
      661 attccagcga gcggagaagg cgagtcgaga agtcgagatc tccgtcctcc gcagattgcg
      721 aatcagttca gatctggtgc gagggttggt gttaggagtc tgatagattt tcagcagaac
      781 caacggcgcg catccatttc cgtgagcacc aggagcagat tcaacagcgc cgcaacagcc
      841 tgagctcgga atgttggcga accacaagcg cgtccgacgg ctccgtttgt agttgtactt
      901 aaaccagacg ccaccgcgcc gaggacagtg agcgtgagcc agctcatcaa gtttttcgcc
      961 aagcgcttat gcggagaccc tttcctcgcg ctgccgcaaa tcacttcctc gccatcgact
     1021 tcaactggtc gagggcgaga ttcacctcta gcacattcac tcgcggctcg cccaggaacc
     1081 caatctggcg cccagacgtg tgctggtcaa tgatctgccg gacctgagct tcgctcaaac
     1141 cacgctcgcg ggcgatgcgc ggaacttgaa actccgctcc agcgggagag acatctggat
     1201 ccagacctga cgcggaagtc gtaaccaagt ccacaggaac cggctggcca gggttctccg
     1261 cttgcagacg cgcaacgtca gccttcacgc ggtcgatcaa gtgctggttc gtcgggccca
     1321 gattcgatcc gccagagttt gccgcgtcgt agccgttgcc tgaccctgcc gccgatagcc
     1381 gggaatgcag gtaccccggc ccgacgaaag cctgcccgat aatccgcgag ccgacgattt
     1441 cctcattgcg cgagacgagc tgcccgttgg ctttgtcgcg aaacatcaac tgcgccagcg
     1501 ctgtgaccac cagaggataa acaaggccca gcagtaccgt catgacgatt gtcatcaaga
     1561 ttgcaatctt aagattttgc ttcataggtc ctcttcttat gctagtccga caccggtgat
     1621 gaacatgtca atcagcttga tcccgatgaa cggtacgata attccgccaa cgccatagac
     1681 ccacaggtta cggcgcaata acgcagcagc gcccatggcc cggtacttca ccccgcgcag
     1741 cgccagcgga atcagcgcaa tgatgatgac ggcattgaaa atcaccgctg acaggatggc
     1801 ggatccaggt gtgcgcaggt gcatgatgtt gagcgcgttc agcacgggga atgttcccgc
     1861 gaacatggcc ggaatgatgg cgaaatactt ggcgacgtcg ttggcgatgg aaaatgttgt
     1921 cagcgacccg cgcgtcatca gcaactgctt gccgatagcg accacctcga taagcttggt
     1981 cggattggaa tccaagtcaa ccatgttgcc ggcttccttg gctgcctggg tgccggtgtt
     2041 catggctaca ccaacgtcgg cctgcgccag cgcgggagca tcgttggtcc cgtcaccggt
     2101 catggcaacg agtttgcctt ctgcctgctc gcgacggatg aggtccatct tttccttggg
     2161 tgtggcttcg gcgaggaagt catccacacc cgcttcgcgc gcaatcgccg ctgccgtcag
     2221 cgggttgtcg
//
LOCUS       DASRKK010000480         7785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_624412, whole genome shotgun sequence.
ACCESSION   DASRKK010000480 DASRKK010000000
VERSION     DASRKK010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624412"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFC15_11175"
     CDS             <1..303
                     /locus_tag="VFC15_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZM10761.1"
                     /translation="VVFVGTIVMTNVISNVATAAFMFPVALSMSEMLGVNFKPFAMVL
                     MVAASCAFVNPAGFQTNLMVQKEGGYTFADFARVGLPLTLLVGIVVLLVAPIVYQF"
     gene            401..1963
                     /locus_tag="VFC15_11180"
     CDS             401..1963
                     /locus_tag="VFC15_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM10762.1"
                     /translation="MNSLSTARMECGRPRRLAFTAATLILCLSACKLLVHLYAGRHYG
                     YFVDELYYLACSRHLAWGYVDQPPLIALITWIARSLLGDSLAAIRFLPAIAGAAEVAL
                     TALLARELGGSRFAQGFAALATLVTPGILASHGLLTMNAFEPLFWMGSAWLLIRIVKT
                     GNQKLWIWFGAVAGFGLENKYSMLIFGAGIVVGLLLTPQRRVFLSPWIWVGGAIAVLL
                     FLPNLLWNIQHHFPFLELQANIRRSGRDVPLSPPAFFGQEILTMHLLTLPIWLAGLWF
                     YFVSPPGKPFRALGWAWVFSAAVIVTFSPRVYYLYPAFPILFAAGAVVWETRLQARPL
                     WQKLAWPVLLVATGALMAPLAIPLLPPETYIRYTETMHLAPPRIETHRLGPLPQIFAD
                     QFGWEEMAVAVAQVYNHLPPDIRSQTAIFGQNYGQAGAIDWFGPKYGLPPAISGHQSY
                     FLWGPRGYTGESMIVMQGRQEDLERFYASVEMVASVFHPYSMPYEHFDIFYCRGLKRP
                     LNEIWPEVKNWD"
     gene            2113..2325
                     /locus_tag="VFC15_11185"
     CDS             2113..2325
                     /locus_tag="VFC15_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10763.1"
                     /translation="MITILGAGGAVANELVKLLAARNQPFRIVGRNPRPTHGASETLA
                     ADLTDKDQTIRAVAGSNIVHLLVGLK"
     gene            2362..3051
                     /locus_tag="VFC15_11190"
     CDS             2362..3051
                     /locus_tag="VFC15_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM10764.1"
                     /translation="MSNTIEACKRAGAKLIFFDNVYMYGKVNGPMTEATPFNPCSKKG
                     EIRANIATTLINGWKAGDLTGMIARAADFYGPDSLDGVPNLLVFEPFSRGKKASWLVK
                     DSVPHSYTYTPDAARSLLLLAERATAWNQTWHVATTPNPSTGKEFVTMVAKELGAEPK
                     YRVLSRPMIRLAGWFDPLVSESYEMLYQSDSPYLFDSSKFANEFGFAGTPYADGVRTT
                     AACYKGKSRGA"
     gene            complement(3374..3556)
                     /locus_tag="VFC15_11195"
     CDS             complement(3374..3556)
                     /locus_tag="VFC15_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10765.1"
                     /translation="MPEERERSAVLIAACLIAAIRLRGEPIQASPKLKATIYDSVQLA
                     VLVWREIQGRGVKPVN"
     gene            complement(3967..5472)
                     /locus_tag="VFC15_11200"
     CDS             complement(3967..5472)
                     /locus_tag="VFC15_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZM10766.1"
                     /translation="MKSQKKLRSLVGVAVVVLLTGCTVGPHYHSPAPPIVGTYTPAPQ
                     ASETVSSAGTAGAVQHLNPAMDVPAQWWTLFHSPEIDRLVREALANSPTLAQATARLK
                     EAQEESNARTGATRYPTVNANVSVQREQVDLATFGVPFPSPPPFSLLNGSVAVSYALD
                     LFGANRRLIEGLNAQAEYQRWQLQAARLMLAGNVVSAAIRQAQLRSQIEITHQILELQ
                     QQQLSITEQRVQAGGLPQYEINRQRTLVEQTRATIPPLEQQVAVVNHQLAVLIGKTPA
                     EAQSDSVNLDSLQLPQELPLSLPSSLARQRPDIRASEALLHQASANVGVATANLYPQI
                     VLSGNVGAIGTNFASGSDVWNVGASLAQPIFNGGALRAEKRKSVAAYEEAGSAYHQTV
                     LQAFREVADALRAIENDAQTLQARTEAAKQAEVTYEIAAQRFNAGGISQLALLDAQRQ
                     HLQTLLDRTASAAIRYTDSASLFQALGGGWWNGTQASAVISTPLRKRTDSN"
     gene            complement(5483..6625)
                     /locus_tag="VFC15_11205"
     CDS             complement(5483..6625)
                     /locus_tag="VFC15_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10767.1"
                     /translation="MRGRNGFSISRWWSIVRKEFLQLRRDRITFAMIVGIPIIQMALF
                     GYAINTDPKHLDTAIISADHSDITRSFISAMKNSAYFKVVGELPDQAAGRTALDQGKV
                     LFVVNIPAGFTRQLLRHERPSLLIEADASDPIAAASALAAVNGITQSVIQKELTGPLA
                     PLAGSPAPFDVEIHRLFNPDGITQYNVVPGLMGVILTLTMVMMTGLAITRERERGTME
                     NLLAMPSIPIEVMTGKLIPYIGIGLIQSTIILLAARYVFHVPFFGNIGAVYLVALLFV
                     AANLTVGITFSSLAGNQLQAVQLAVFYFLPNILLSGFMFPFQGMPKWAQVIGNLLPLT
                     YFNRLVRGILLKGNGWGNSWPNVWPVMIFTLVVMLAAVKTYRRTLD"
     gene            complement(6622..7569)
                     /locus_tag="VFC15_11210"
     CDS             complement(6622..7569)
                     /locus_tag="VFC15_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM10768.1"
                     /translation="MNATANPEYAIDVRDLNKHFGDKQVVNNVSMQVRKGEIFGFLGP
                     NGSGKTTTIRMMCGLLKPDSVSGTCLGFDIRRDSAEIKRNVGYMTQRFSYWDDLTIRE
                     NLDFVARMYQVPNRRDVVQKSLEDLGLSSRAGQLAGELSGGWKQRMALAACMLHNPML
                     LLLDEPTAGVDPKARRDFWDELQGLAARGVSVLVSTRYMDEAVRCHKLAYIAYGELLA
                     QGTAAEVVASQHLSSWAIFGDHLVELQETLRQQPGVEQTVSFGTVLHVSGTDAATLQL
                     TIDAIVAAKGLRAEKIDTGLEDVFIYMMSRSDDNFGDRA"
     gene            complement(7566..>7785)
                     /locus_tag="VFC15_11215"
     CDS             complement(7566..>7785)
                     /locus_tag="VFC15_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10769.1"
                     /translation="LKLEQGVSVHCDGCASEFPATVTFISTQCEFTPPVIYSNENRSK
                     LVFLVIAKPPVDKATALHPGQPLEVTLQ"
BASE COUNT         1587 a         2324 c         2144 g         1730 t
ORIGIN      
        1 gttgtattcg tcggcaccat cgtcatgacc aacgtcatta gcaacgtcgc taccgccgct
       61 ttcatgtttc ctgtcgcctt atcaatgtca gaaatgctgg gcgtcaactt caagccgttc
      121 gcgatggtgc tcatggttgc cgcctcgtgc gccttcgtca atccggccgg attccaaacc
      181 aacctgatgg tccagaaaga aggcggctac acctttgccg atttcgccag agtcggactc
      241 ccgctcaccc tcctggtcgg aatcgttgtg ctgctggtgg cccctatcgt ctatcagttc
      301 taaataccgt tgcgattcca gcagttcgaa ctcagcgaac gcttacggaa gaaggctctt
      361 ctcacttggg cagagctgta tagttcgcaa tagaagtccg atgaattcac tatccactgc
      421 gcgcatggaa tgtggccgtc ctcgccgcct ggcgttcacc gccgcaacct taatcctctg
      481 cctgtccgcc tgcaaacttt tggtccattt gtatgctggg cggcactatg gttatttcgt
      541 cgatgagttg tattaccttg cctgcagtcg ccatttggct tggggttatg tggatcagcc
      601 gcccctgatt gcgttaatca cgtggatcgc gcggtcactg ctgggtgatt cgttggcggc
      661 gatccggttc ttgcctgcca tcgcaggagc ggcggaggta gctctcaccg cactgctggc
      721 gcgcgaactc ggaggcagtc ggtttgcgca aggattcgcc gcgctcgcca cactggtaac
      781 gcctggcatt ctggcctccc atggcctgct gaccatgaat gcattcgaac cgctgttctg
      841 gatgggtagc gcctggctgt tgatccggat tgtgaaaacg gggaatcaaa agctttggat
      901 ctggttcggc gcggttgcgg gtttcggcct ggaaaacaag tactccatgc tgatcttcgg
      961 ggcaggaatc gtggtcggcc tgctgcttac gccgcagcgg cgggtgtttc tcagcccatg
     1021 gatatgggtc ggcggcgcga tcgcggtgct cttatttctg ccgaacctgc tgtggaacat
     1081 tcagcaccac ttcccgttcc tcgaactgca ggccaacatc cggcgaagtg gtcgcgatgt
     1141 tccgctttcg ccaccggcat tctttggtca ggaaatcctg accatgcacc ttctcactct
     1201 gccgatctgg ctggctggac tgtggtttta cttcgtctcg ccaccgggca agccgtttcg
     1261 cgcgctcggc tgggcttggg tcttctccgc agcagtcatc gtcacgttca gtccgcgcgt
     1321 ctactacctg tatcctgcgt ttccaatcct atttgccgcc ggggccgtgg tttgggagac
     1381 tcgccttcag gccaggcctt tgtggcagaa gcttgcgtgg ccagtcttgc tggtcgccac
     1441 cggagcattg atggcgccac ttgcgattcc gcttttgccg cctgaaacct acatccgcta
     1501 taccgagacg atgcacctag cgccccctcg catcgaaacc catcgactgg gcccgttgcc
     1561 gcagattttt gccgatcagt tcggctggga ggagatggcg gtcgcggtcg cgcaggtcta
     1621 taaccacttg ccacccgaca ttcgctctca gaccgcgatc ttcggccaga actacggaca
     1681 agccggggcc atcgactggt ttggtccaaa gtacggcctg ccgcccgcca tctctggcca
     1741 ccagagttac ttcctctggg gacctcgtgg ctacaccggc gaaagcatga tcgtcatgca
     1801 ggggcgtcag gaagatctgg agaggttcta cgcctcagtt gagatggtag ccagcgtttt
     1861 tcatccctac tcgatgccgt acgagcattt cgatatcttc tattgtcgcg gcctaaagcg
     1921 gcccctaaac gaaatctggc ccgaagtaaa gaattgggac tgagagcgca gcagccccgc
     1981 acggacgttg cgcgcaagat gaaccgggcg gtaagtggca aatcgagtga taggtgcgga
     2041 gcaatggcca ttacttcaag gaccgctcaa tcggctaggc aatacttgtc tccgcgcaaa
     2101 ggcaggattt gaatgatcac cattcttgga gcaggcggtg cggttgcgaa cgaactcgtc
     2161 aaacttctgg ccgcaaggaa tcagcctttc cgcatagttg gccgcaatcc tcggccgacg
     2221 catggcgcga gcgaaacact cgccgccgat ctcacggata aagaccagac catccgcgcc
     2281 gttgccggtt ccaacattgt gcatctgctc gttggtctca agtaagatca caaggtatgg
     2341 caggaaatgt ggccgcgtgt catgagcaac acgatcgaag cctgcaagcg tgctggtgcc
     2401 aagctcatct tctttgacaa cgtgtatatg tacggcaagg tcaatggtcc catgaccgaa
     2461 gcgacgccct tcaatccctg cagcaagaag ggcgaaatac gcgccaacat agccacaaca
     2521 ttaatcaatg ggtggaaggc cggcgatctc accggcatga ttgcgcgcgc ggctgatttc
     2581 tatggtcctg actcgctcga tggagttcct aaccttctcg tcttcgagcc tttctcgcga
     2641 gggaagaaag cctcatggct ggtgaaggat tctgttccgc actcctacac ctacacgccg
     2701 gacgccgcgc gaagcctgtt actgctcgca gaacgggcga ccgcatggaa ccagacctgg
     2761 catgtggcca ccacgcccaa tccgtccaca ggaaaagagt tcgtcaccat ggtcgcgaaa
     2821 gaactcgggg ctgagccaaa atatcgtgtg ctgagcaggc ccatgatcag gcttgccgga
     2881 tggtttgatc cgctggtatc ggaatcctac gaaatgctct atcagagtga ttctccctac
     2941 ctctttgact caagcaagtt tgccaacgag ttcggcttcg caggcacacc gtacgccgac
     3001 ggcgttcgta ccacggccgc ctgctataag ggcaaatcgc gcggtgccta gaacaagaga
     3061 cagtcacaac gctatgatca tccgcgatgc aatgccaaca ttgctacgct tctgttggta
     3121 ttgcccaagg gaacgcggag ttggtgttct acgttgcgct cgagtgacgg gcgatcctca
     3181 agtaaccgca tagggaagaa cgcccgaagt cgcagagcaa cggacgcacc ttctccccct
     3241 ccggggttat cgctaccgta gctttagccc agtcgatcct gattgcttcc gccagccctc
     3301 gaagcctctc cggtccgcag cagctgggct gaagaaagcc atgtcagcca tcaaaacctc
     3361 ctcggtgcaa acgtcagttt actggcttca cgccgcgtcc ctgtatctca cgccagacca
     3421 gcaccgcgag ctggacggaa tcgtaaattg tcgccttcaa cttggggctg gcctggatag
     3481 gctctccccg cagccgaatc gctgcaatca agcaggcggc tatgagcact gcactacgtt
     3541 cacgttcttc cggcatggca ctcagtgtag gcgaacaaaa gacgaatcgt caaccccgcg
     3601 aatatccgtt gaaatccatg cagcccgggc ggccaaccgt attaagcggg ctggtcatga
     3661 gacatgcctt tatgagcctt tttattcttg ttcagtctcg taatttctgt gccttaactg
     3721 tgccttacaa gaccgaacaa ggtggctttt ccacagacat tttcgcaaat cctaaatggc
     3781 taagtagttg taaacaaaca aaacaatcca cttacaaaag ttggttgtag gtttaggaaa
     3841 ccgctgctct atccatctga gctacagggc cagtggattt aacacaatta tcgcacgcag
     3901 aattaccgtc aggcagcctc gcgccgtttg ggcaagatac cctgcccgat tattgtcctg
     3961 gttcggttag tttgagtcgg tgcgtttgcg caatggagtc gaaattactg cgctcgcctg
     4021 cgtaccattc caccaccctc cgcccagtgc ctggaaaaga gacgccgaat cggtgtaccg
     4081 gatggctgcc gaggcagttc gatccaacag ggtttgcaga tgctgccgct gggcatcgag
     4141 cagcgccagt tggctgatgc ccccagcgtt gaagcgttgc gcagcgatct cgtaggtgac
     4201 ctcggcctgc ttcgcagctt ccgtccgagc ctgcaacgtc tgagcgtcgt tttcgatggc
     4261 gcgcagtgcg tccgctactt cgcggaaagc ctgtagcacg gtctgatggt acgcactacc
     4321 tgcttcctca taagccgcga cggatttgcg cttctcagcc ctcagcgctc caccgttgaa
     4381 gatgggctga gccagggatg cgccgacgtt ccatacatcc gacccgctgg cgaaattcgt
     4441 cccaatagcg cctacattcc cagatagaac aatttgtgga tagaggttcg ctgtggcaac
     4501 cccaacattc gcactggcct ggtgcagcaa tgcctcagag gcccgaatgt ccggacgttg
     4561 gcgcgctagc gacgacggca ggctgagtgg caattcctgc ggaagctgca agctgtcgag
     4621 gttgacgctg tcgctttgag cttcggccgg agtcttgcca atcagcactg cgagttgatg
     4681 gttcaccacc gcaacctgct gttccagtgg aggaatggtc gctcgtgtct gctccaccaa
     4741 ggtgcgctgt cggttgatct cgtactgggg tagtccaccg gcctgtacac gttgctcggt
     4801 gatgctgagt tgttgctgct gcaactccag tatttggtgt gtgatttcga tctgcgagcg
     4861 caattgcgcc tgccgaattg ctgcggaaac cacgttacct gccagcatca atcgcgccgc
     4921 ctgcagttgc caccgctgat actcggcctg cgcattgagg ccctcgatca gccggcgatt
     4981 cgcgccaaac aaatccagcg catacgacac cgcgaccgat ccgtttagca aactgaacgg
     5041 tggtggactg gggaagggaa ctccgaaagt cgcaagatcg acttgttctc tttgcaccga
     5101 aacattggcg ttcaccgtcg gatatctcgt ggcgccagtt cgggcgttcg attcttcctg
     5161 cgcctccttg agacgcgccg tggcttgcgc cagggtgggg ctgttggcga gggcttcacg
     5221 caccaggcgg tcgatttcag gggagtggaa cagcgtccac cattgcgctg gaacatccat
     5281 cgccggattg agatgctgca ccgccccagc cgtacctgca gagctgaccg tctccgacgc
     5341 ctgcggtgct ggcgtgtagg tgcctacgat cggcggggcg ggcgaatgat agtgcggtcc
     5401 gaccgtacat cccgtcaata gcacaacaac tgccacgccc accaatgatc tgagtttttt
     5461 ctgtgacttc atagctcata cctcaatcca gcgtccttct atatgtcttt accgccgcca
     5521 gcatgaccac caacgtgaaa atcatgaccg gccacacgtt cggccacgaa ttcccccatc
     5581 cgttcccctt cagcaggatg ccgcgaacca ggcgattgaa ataggtcagt ggcaacaggt
     5641 ttccgatcac ctgcgcccac ttcggcatac cttgaaaggg aaacatgaag ccggagagca
     5701 atatgttagg cagaaaatag aagacagcca gttgcaccgc ctgcaactgg ttcccggcca
     5761 gcgacgaaaa tgtaatgcca accgtcagat tggcagccac gaacagcagt gcaaccaagt
     5821 ataccgctcc gatgttaccg aagaagggaa cgtgaaacac atagcgcgcg gccagcaaaa
     5881 tgatggtcga ctggataagt ccgatcccga tatatgggat cagcttgccg gtcatcacct
     5941 caatcggaat cgacggcatg gccaacaggt tctccatcgt gccgcgctcg cgttcgcggg
     6001 tgatggcaag tccagtcatc atcaccatgg tgagcgtcag tatcactccc atcagcccgg
     6061 gcactacgtt gtactgcgta attccgtcgg ggttgaacag tcgatgaatc tcgacgtcaa
     6121 atggcgctgg gctacccgcc aggggagcca gtggcccagt cagttctttc tggatcaccg
     6181 attgcgtaat gccattcacg gcagcgagcg cgctggccgc tgcgatcgga tcgctggcat
     6241 cggcttcgat cagtagcgat ggccgctcat gacgcagcaa ctgccgggtg aatcccgccg
     6301 gaatgttcac cacgaacagc accttgccct ggtccagggc cgtgcgcccg gcagcttggt
     6361 ccggcaattc tcccacgacc ttgaagtacg cggaattctt catcgccgag ataaaactac
     6421 gggtgatatc gctgtggtcg gcgctgatga tggccgtatc caggtgcttg ggatcggtgt
     6481 tgatggcata gccgaacagg gccatctgaa tgataggaat tcccacgatc atcgcgaacg
     6541 taatgcggtc acgccggagc tgcaggaact ccttgcgaac aatgctccac cagcgactga
     6601 tcgagaaacc gttgcgccct ctcatgcccg gtctccgaag ttgtcgtccg aacggctcat
     6661 catgtagatg aacacatcct ctagcccggt atcgatcttt tccgcgcgca gtccctttgc
     6721 ggcgacaatt gcgtcaatcg taagttgcaa agttgcagca tcggttccgc tcacgtgcag
     6781 caccgtgcca aaggaaacag tctgctctac tcctggttgt tggcggaggg tttcctgcaa
     6841 ctccaccagg tgatcgccga atatcgccca gctgctgaga tgttggctgg cgactacttc
     6901 cgcggctgtg ccttgcgcca gaagctcgcc gtatgcgatg taggctagct tgtgacagcg
     6961 cacggcctcg tccatatagc gcgtgctgac cagcaccgag acgccgcgcg ccgccaatcc
     7021 ctgcaactcg tcccagaaat cgcgccgcgc cttgggatcg actcccgccg tcggctcatc
     7081 gagcaggaga agcatgggat tgtgcagcat gcaggccgcc agcgccatgc gctgtttcca
     7141 gccccccgac agctcgcccg ccaattgccc agctcggctg gaaagtccga gatcctccaa
     7201 cgacttctgc accacatcgc ggcgattggg aacctgatac attcgggcca cgaaatccag
     7261 gttctctcgt attgtgaggt catcccagta ggaaaatcgc tgcgtcatgt agccgacgtt
     7321 gcgcttgatc tccgcgctgt cgcgtcggat gtcgaatccc aaacatgtgc cgctgaccga
     7381 gtccggcttt agcagtccgc acatcatacg gatagtggtt gtcttaccgc ttccgttggg
     7441 acccagaaac ccgaatatct cacccttccg cacctgcatc gacacgttgt tcaccacctg
     7501 cttgtcgccg aagtgcttat tcaaatcccg cacatcgatc gcgtattccg gattggcagt
     7561 tgcgttcact gcagcgtgac ctctaagggt tgtccgggat gcagcgccgt agctttgtcg
     7621 accggcggct tggcaatcac caggaacacc agcttggagc ggttctcgtt gctgtaaatc
     7681 accggcggcg tgaactcgca ttgtgtcgag atgaaggtaa cggttgccgg aaactcggac
     7741 gcgcagccat cgcagtgcac actgacgcct tgctccaact tgagc
//
LOCUS       DASRKK010000481         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1407277, whole genome shotgun sequence.
ACCESSION   DASRKK010000481 DASRKK010000000
VERSION     DASRKK010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407277"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1062
                     /locus_tag="VFC15_11220"
     CDS             172..1062
                     /locus_tag="VFC15_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10770.1"
                     /translation="MTRLRKMMLEELERRNYSAGTTRRYLRFVERFAQHFGKSPDQLG
                     PDDLRSYQAYLLRELKLCPGSVENHVAALRFFFVRTLHRHQFREFLPYPKTRRKLPNI
                     LSQEEVARLINASSSLFQRTLLMVLYGTGMRRSELARVKIAHIDSQRMIIHVVDGKGH
                     KDRDLPLSPTLLDNLRVYWRWLKPHTYLFPSRLHRDQEQPISDKAVWVACTEAARKAG
                     LRKPVSPHLVRHTWATHLLEAGTDLRTIQLLLGHEDLEVTARYLHLSAQHLQKVTNPL
                     EGLKLSSVDESSRLYHRPRS"
     gene            1059..2231
                     /locus_tag="VFC15_11225"
     CDS             1059..2231
                     /locus_tag="VFC15_11225"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HZM10771.1"
                     /translation="MTRPAIEVADIVRRKGKQFLERFKAVLSYQQLKAYRAVERCRTA
                     ALGGHRDKCEDCPYEAPFSYNSCRSRCCPKCQAQARKRWLQIQQRDLLNTNYFHVVFT
                     LPHELNPLALTSRRPLLELLFDASSRTLLEVAADPKRLGAEIGFLSILHTWGSNLLPH
                     YHIHAVVPGGGLSADHQRWIHTTHPMFLLPVPVLRTVFRKKFLAGLHHLYRKGLLNLR
                     GPAAPLRDPALFDQLIEKLGNKNWYVHAEPPFGGPEHVLRYLGRYTHRMAISNHRITA
                     FDGERVSFRWRDYAHGGKQRVMTLDAVNFLGRFFLHVLPKSFVRIRRYGLLSNRFRKQ
                     LLPLARTLLAAQGREPLSLPPLPDCAPWHCPRCGKAMRVVQRYTAAQLYFAGFDTS"
BASE COUNT          629 a         1030 c          804 g          637 t
ORIGIN      
        1 gtatcaacca ctccgaacgc gtgccgaagt gcgcgtgccg aagtatttac tgacgggtaa
       61 ctgacgagta ggcggtcaga acgaagccgc aggcgccgcg gtcgctgcag tattggctga
      121 cgagtagttt tgtggccggg atagtgtgtg agtcacagga ggtaacacac tgtgactcgt
      181 cttcgcaaaa tgatgctcga agagctcgag cgtcgcaact actctgccgg cacgacgcgg
      241 cgttatctgc gtttcgtcga acggttcgca caacacttcg gcaagtcgcc ggaccagctc
      301 ggtccggatg atctgcgcag ctaccaggcc tacctgctca gggagctcaa gctctgtccg
      361 ggaagcgtcg aaaaccacgt ggccgcattg cggttcttct tcgtgcgcac gcttcaccgc
      421 catcagttcc gcgagttcct gccctatccc aagacacgga ggaagctgcc caacatcctc
      481 agccaggaag aggtcgcacg cctgatcaac gccagcagca gtctgtttca gcgcacgctg
      541 ctgatggttc tttacggaac cggcatgcgc cgttccgagc tcgcccgggt caagatcgcc
      601 cacatcgaca gccagcgcat gatcatccat gtggtcgacg gcaaggggca caaggaccgc
      661 gatctgcccc tgagccctac cttgctcgat aacctgcgtg tctactggcg atggctcaag
      721 ccccacacct acctgttccc gtcacgactg catcgcgatc aagagcagcc catcagcgac
      781 aaggcagtgt gggtggcgtg caccgaagca gccaggaagg ccggccttcg caagccagtc
      841 agtccgcatc tggttcgaca cacatgggcc acgcatctgc tggaagccgg taccgacctg
      901 cgcaccatcc agttgctgct cggccatgag gatctggagg tgacagcccg ctatctgcac
      961 ctgtccgccc agcacctaca gaaggtcacc aacccccttg agggactgaa gctctcgagt
     1021 gttgacgaga gcagccgcct ctatcatcgt ccgcgttcat gacgcggccg gccatcgaag
     1081 tggccgacat cgtccgtcgc aagggcaagc agtttctgga acgcttcaag gctgttctca
     1141 gctatcaaca gctgaaggct tatcgggccg tggaacgttg ccgaactgcc gcccttggcg
     1201 ggcataggga taagtgtgag gactgtccgt acgaagcacc cttctcttac aactcatgcc
     1261 gatcgcgctg ttgtccaaaa tgccaggcgc aggctcgcaa gcggtggctt cagatccagc
     1321 aacgcgatct gttgaacacc aactacttcc acgtcgtctt caccctgccc cacgaactga
     1381 acccactcgc actgacttca cgcagaccac tgctcgagct gctcttcgat gccagttccc
     1441 ggaccttgct cgaagtcgcc gccgatccca aacgcctggg cgcagagatc ggcttcttat
     1501 ccatcctgca cacctgggga tccaacctgc tgccccatta ccatatacac gcagtcgtcc
     1561 ccggcggcgg actgtcagcc gaccatcagc gctggatcca caccactcat ccgatgttcc
     1621 tgctgccggt tcccgttctg cgcaccgtct tccgcaagaa gttcctcgca ggccttcacc
     1681 atctctaccg caagggcctg ctcaacctca gaggtcctgc cgctcctctc cgtgatcctg
     1741 cgctgtttga tcaactgatc gagaagcttg gcaacaagaa ctggtacgtc cacgccgagc
     1801 cgcccttcgg cggccccgaa catgtcctgc gctacctcgg ccgctacacc caccgcatgg
     1861 ccatctccaa ccaccgcatc accgctttcg acggggagcg cgtcagcttc cgctggcgcg
     1921 attatgccca cggcggcaag cagcgcgtca tgactctgga cgcggtgaac ttcctcggcc
     1981 gcttcttcct gcacgtattg cccaaaagtt tcgtgcgcat ccgccgctac ggcctgctgt
     2041 cgaatcgctt ccgtaaacag cttctgccac tggcacgaac actgctcgct gcacagggcc
     2101 gcgaaccgct gtctcttccg ccactgcctg attgcgcacc ctggcactgt cctcgctgcg
     2161 gaaaagccat gcgcgttgtc caacgttaca ccgctgcaca actctatttc gcaggcttcg
     2221 atacttcatg aacgctcgtc ccagctcgcg ccgccggctt gctcctcgca cgtctttacg
     2281 gcagtgtgcg ccaagccctc agaacggctc catttctacg ctcaatcgca tcttgaaacc
     2341 attaaatcga agttcgctct gctatccgct cactcgcttc catcatcaac cgcaaaaaaa
     2401 cgacccacag cggtactcac caaaagccca aatgtcattc aataaccata atttcatcta
     2461 agatcaccgc gaccgcggcg cctgcggctt ccttcttacc tccaaatcga aaacgcctcg
     2521 cactcaattc tgaggctcac ccctaactcg tcgccgaggc gtttccgatg ttggctaacg
     2581 gtaatccact cggagccgcg attgcaatgc aaatggctcg ctgagcgcct cgatttgtca
     2641 ttaattgaca tcggggcaga gagagtgggt cataactctt tcgtaccttc cgaaaactcc
     2701 gttttgctgc aggttcggcg cgcgagacca atcggcgacc gcagcggagt gcctacagcg
     2761 ttttcaagaa ggcgatgcaa gaccaaggct cacctggtga ttcgtagaga agacctggct
     2821 aggtggagag gtaggcggca ggtttacccc gcccctctgt cctcattttg ccgtggctgg
     2881 gcggagatag cctagaggag agctcgcggt gcgtctgctc ctgctgccac gttgcctctc
     2941 gattcggcgg cccacataca gacaatcgct ctggcccgtc gaaaaaggac cgaggatcgt
     3001 cggccctttc ttgaatccgt aagtcccgca ggcgcagcta ttcgttcttg aatccgttcg
     3061 aatatccctt gacgaacgac tcgcggaact gcagcttgaa
//
LOCUS       DASRKK010000482         6104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_917929, whole genome shotgun sequence.
ACCESSION   DASRKK010000482 DASRKK010000000
VERSION     DASRKK010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917929"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFC15_11230"
     CDS             <1..193
                     /locus_tag="VFC15_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10772.1"
                     /translation="KWRPSLVDHDKTAFPLKGAHQNVRCGACHKSFRPFEGKDVLFYK
                     PTPTECAACHGNAVAKIAM"
     gene            232..2160
                     /locus_tag="VFC15_11235"
     CDS             232..2160
                     /locus_tag="VFC15_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10773.1"
                     /translation="MPTLIPLFFSLLGMLLPVPMGQQSQAVPRAQIQSPHGNLNMPCE
                     NCHTFTSWRPIRNIPEFNHDKTRYPLRGMHANVSCMQCHTSLVFSNVGMRCKDCHADL
                     HRGQFGAACEQCHTVRGWGVSLSKIQRHDNRFPLIGGHAAVECDACHKNAAVGQFQGL
                     SVNCYSCHQVQYQNAVPDHKATGFPPTCQNCHSSVDTWLGAKFDHAKFTGFALTGMHA
                     TLDCAACHVGGRYKGTPANCYGCHAKDYNATNNPNHLQAGFPRECATCHSTATWSGAK
                     FDHSRTKFPLTGMHTNVPCTSCHINGKFAGTPTDCASCHITDYNKTTNPNHKASGIPT
                     TCAVCHSTNGWIPAVFDHSKTKFPLTGVHTTVACTACHINGRYAGTPTDCASCHITDY
                     NKTTNPNHKAAGFPTDCSICHSTSGWSPASFDHSKTKFPLTGKHATLPCVACHVNGNF
                     TNLQTTCVSCHLNDYNGTTDPNHKAAGFPTDCQVCHSTSSWDGAKFDHSKTGFPLTGA
                     HITVPCVKCHINGVYRGTPTDCYSCHKADYLGTNNPNHVAAHFPTTCATCHTTATWLG
                     AVFNHTWFPIYSGTHAGRWMTCGDCHINPADYSAFSCITCHEHSKQNTDPKHTEVRGY
                     VYNATSCYSCHPTGQGGG"
     gene            2163..2726
                     /locus_tag="VFC15_11240"
     CDS             2163..2726
                     /locus_tag="VFC15_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10774.1"
                     /translation="MVQRITSLLLLAWLTQAGVAQAPQTATTTPYIAQPTAPIEMHTT
                     FRVRYVNGANVYIEGGRSTDLVEGTRLVVRAGAASTTALSAESESHQPADAGNPSDGA
                     PGLRTIAELKVVAVAETSAVCEVISSTRPVVAGDVATLPQEEIEKLVEKHALSNTRNY
                     PAVVSFTEGDPWMRTYATPYHGHLCPK"
     gene            2675..3973
                     /locus_tag="VFC15_11245"
     CDS             2675..3973
                     /locus_tag="VFC15_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10775.1"
                     /translation="MDEDVRDSIPRPPLPEVNRAQGRIGFDFSTIHNGGPFSTSSTTT
                     GVVLRADITRINGTHWNLNGYWRGGLTSTSSRNQPTLQDLLNRTYTISLTYQNPDSRW
                     VAGMGRLYLPWASSLNSFDGGYFGRRMSDRVTAGIFGGLASDPTALSYDPNRRIAGGF
                     TNVSGGSYDDVHYSSTFGAGVGMLKWSVDRPFVFTENTIDYKRVFSVYEALLLDRPRT
                     GPGVAPVGAGVASSFVTFRVQPHPRVSFDFNHSYFRDVPTYDPQLVGTGLLDKYLFQG
                     FSAGVRVEVPAHITLYTEIGRSHVTGDAKASWNTMYGITFNRIWKTGIRLDARYSNFN
                     SPFAQGDYRSFSVSRSFKDSFQIQVQAGLQKFISPVSKDNGSRFLNAYGEFVLGRHYF
                     IDTGFTITRGALQNYNQVYTTFGYRFDNRWKREAASATQK"
     gene            3957..4769
                     /locus_tag="VFC15_11250"
     CDS             3957..4769
                     /locus_tag="VFC15_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10776.1"
                     /translation="MRHKSNGWFRRCLFVMTLVLRCAPPAWAADADTIVDRASRQVTA
                     FLDQMSDVKCSEHVTQLKLDKNGHTAYLENSSFDYLVLLQGSGDELLLNESRLSDEHK
                     EQRNKKNVPLLITNGFSMLMLIFHPYYRNSFEFAAMPDEIIAGHRTAQVRFTHIPGTR
                     TPAALAVRGREFPLELTGTAWIEPETGQVVKLKIGLAKDMSDVGLKSLTAEIDYDPIH
                     LPGWTQAYRFPAVATVEVETLRQRWRNVHRFSNYKRFLVDTSVTVVDPEQKK"
     gene            4766..5707
                     /locus_tag="VFC15_11255"
     CDS             4766..5707
                     /locus_tag="VFC15_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10777.1"
                     /translation="MSAPASIMNYGSVQHTRERAHRMRLHLAALTAVVSVVALAVYGW
                     NYYLLAAAERPFSPKHELLKPSGSIAIKLGIAGVMLFMMIFLYPLRKRIKWLGRIGSA
                     RHWLDFHVIAGLTAPVLIAFHASFKFRGIAGIAFWIMLSVAISGVIGRYIYAQIPRSL
                     SSAELSLKELAEIEDDLSKDLSVQGPLSAQDFASLLQVPTPAQVREMSAYRAILLMMV
                     LDLCRPFYIARLRVRVLGWSRVLPYFGGLIRTPHEDLERAIRIARSKAALSKRIAFLS
                     RTQQVFHLWHVIHRPFSYSFAVLALIHIVVVIALGFV"
BASE COUNT         1298 a         1881 c         1699 g         1226 t
ORIGIN      
        1 caaatggagg ccgtcattgg ttgaccacga caagactgcg tttccgttga agggagcaca
       61 ccagaacgtc cgctgcggcg cttgccataa gagctttcgc ccgttcgaag gaaaggatgt
      121 cttgttctat aagccaacgc cgaccgagtg cgctgcttgc cacggcaacg cagttgcgaa
      181 gatcgcgatg tgagcgagtc atgactgaat tgagaacgtg agataaaact cgtgcctaca
      241 ctgataccgc tgtttttctc gctgcttgga atgttgctgc cggtccccat gggccagcag
      301 tcgcaggcgg tcccgcgcgc gcaaatccaa agtccgcacg ggaacctcaa catgccttgc
      361 gagaactgtc acacgttcac ctcgtggcgg cccatccgca acatccccga gttcaatcac
      421 gataaaacca ggtacccgct gcgtggcatg cacgccaacg tgagttgcat gcagtgccac
      481 accagcctgg tgtttagtaa cgtgggaatg cgttgtaagg attgtcacgc cgaccttcac
      541 cgcggacagt ttggcgccgc ttgtgaacag tgtcatacgg ttcgcgggtg gggcgtttcg
      601 ctgtcaaaga tccagcgcca cgataatcga ttccccctga ttggcgggca cgccgcggtg
      661 gaatgcgacg cctgtcacaa gaacgcggcg gtcggacagt tccaagggct gtcggtgaac
      721 tgctactcct gccaccaagt tcaatatcag aatgcagtac ccgatcacaa ggcgacgggt
      781 ttccccccga cgtgccagaa ctgccattcc tcggtggata catggctggg agcgaagttc
      841 gatcacgcga agtttaccgg cttcgctttg acgggaatgc atgccacgct cgattgcgca
      901 gcgtgtcacg ttggtggccg ctacaaaggg acgcctgcta attgttacgg ctgtcacgcc
      961 aaggactaca acgccaccaa caatccaaac catttacagg ctggttttcc gcgcgagtgc
     1021 gcaacctgtc actctacggc cacctggagc ggagccaaat tcgatcactc cagaaccaag
     1081 ttcccgctta cggggatgca taccaacgtg ccctgcacgt cgtgccacat caacgggaaa
     1141 tttgccggca cgccgacgga ctgcgcctcg tgccacatca cggactacaa caagacgacc
     1201 aatcccaacc acaaagcgtc cggtatcccc acgacttgcg ctgtttgcca cagtacgaac
     1261 ggatggattc cggcggtgtt cgatcactcc aaaaccaagt tcccactcac cggtgtgcat
     1321 accacagtgg cctgcacggc gtgccacata aacggcaggt atgcgggcac gccgacagac
     1381 tgcgcgtcat gtcacatcac cgactacaac aaaaccacca accctaacca caaggcagcc
     1441 ggatttccga ccgattgctc gatttgccat tcgaccagcg gatggagtcc ggcctccttc
     1501 gatcactcca agacgaagtt tccgctcacc ggcaagcacg caacgctccc atgcgtcgca
     1561 tgccatgtga atggcaactt caccaacctc cagaccacat gcgtgtcgtg ccatctcaac
     1621 gactacaacg gcaccaccga tccgaatcac aaagcggcgg gatttcccac ggactgccaa
     1681 gtctgccatt ccaccagttc ctgggatggc gcgaagttcg accacagcaa aacgggattt
     1741 ccgcttaccg gcgcgcacat caccgtgcct tgcgtgaagt gtcacatcaa cggcgtgtat
     1801 agaggcaccc ccaccgactg ctattcgtgc cataaggcgg actacctggg cacgaacaac
     1861 cccaaccatg tggcggcaca cttcccaacg acatgtgcga cctgccacac cacggcaacc
     1921 tggctgggag cggtcttcaa tcacacttgg ttccccatct attcgggaac gcacgcgggc
     1981 cgctggatga cctgcggaga ctgccacatc aacccggccg actattcagc tttttcgtgc
     2041 atcacgtgcc acgagcacag caaacagaat actgacccca aacacaccga agtgcggggc
     2101 tacgtgtaca acgcgaccag ttgctactcg tgccacccca ctggccaggg aggtggatga
     2161 cgatggtcca gcgcataact tccctattgc tgctggcgtg gttaacgcaa gcgggtgtcg
     2221 cgcaggctcc acagacggcg accacgacac cgtatattgc gcaacccacg gcacctattg
     2281 aaatgcatac gaccttccgc gtgcgctacg tcaacggcgc caatgtgtat atcgaaggag
     2341 gtcgcagcac cgacttggtg gaaggcactc gtctggtggt tcgcgcaggc gctgcttcca
     2401 ccacagcgct gtcagccgag agcgaatcgc atcaacccgc agacgctggg aacccttcgg
     2461 acggcgctcc cggcctgcgc acgatcgccg aactgaaggt cgttgccgtc gctgaaacgt
     2521 ccgccgtctg cgaggtgatc tcatccacgc gcccggtggt ggcaggcgac gttgctacgc
     2581 tgccgcagga ggagatcgag aaactggtgg aaaagcatgc cctgagcaac acccgcaact
     2641 atcccgcggt agtgagcttc acggagggcg acccatggat gaggacgtac gcgactccat
     2701 accacggcca cctttgcccg aagtgaaccg tgcccaggga cgcattggct tcgacttcag
     2761 cacgatacac aatggcgggc cgttcagtac gtcctcaacc accaccggag tggtcttgcg
     2821 cgccgacatc acgcgcatca acggcactca ttggaacttg aacggttact ggcgcggggg
     2881 gttgacttcg accagcagcc gaaaccaacc gacgctgcag gaccttctta accgtaccta
     2941 caccataagc ttgacctacc agaaccccga ctcccgctgg gtcgccggaa tggggcgctt
     3001 atacctcccc tgggcgagca gcttgaactc ctttgacggc ggctacttcg gccggcggat
     3061 gtcagaccgg gtaacggccg gaatattcgg cggattggca tccgacccga ctgcgttgag
     3121 ctacgacccc aaccgccgta tcgccggtgg tttcaccaac gtcagcggcg gcagttacga
     3181 cgacgtgcac tacagcagta cgtttggcgc cggagtaggg atgttgaagt ggagcgtgga
     3241 ccggccgttt gtctttaccg aaaacacgat tgattacaag cgtgtctttt cggtttacga
     3301 ggcgctgcta cttgaccggc cccgaactgg acctggtgtg gccccggtcg gtgctggcgt
     3361 ggccagcagc tttgtcacct tccgcgttca accccacccg cgtgtcagct tcgacttcaa
     3421 ccatagctac tttcgcgatg ttcctaccta cgatccgcaa ctggtgggga ctggtttgct
     3481 cgacaagtat ctatttcagg gcttcagtgc aggcgtacgg gtggaggttc ccgcccacat
     3541 caccctgtac accgagatag gccgcagcca cgtcaccggc gatgcgaagg cttcatggaa
     3601 caccatgtat ggcatcacct ttaaccggat ctggaagact gggattcgcc tggatgcccg
     3661 ctactcgaac ttcaacagcc cgttcgccca aggcgactat cgttccttct ccgtgtcgcg
     3721 aagtttcaag gactcctttc agattcaagt tcaagccgga ctccagaagt tcatctcacc
     3781 ggtgtcgaaa gacaacggct cgcgctttct caacgcctat ggcgaatttg tcctgggccg
     3841 ccactacttt atcgataccg gattcactat tacgcggggc gcgttacaga actacaacca
     3901 ggtgtacacc accttcggtt atcgcttcga taatcgctgg aagcgggagg cggcaagtgc
     3961 gacacaaaag taacggctgg ttccgtcgat gtctgttcgt catgacgctt gtattgcgat
     4021 gcgcgccgcc agcgtgggct gcggacgccg acaccatcgt ggaccgggcc agccgtcagg
     4081 tcactgcgtt tctggatcag atgtcggacg tgaaatgttc cgagcacgtg acccaactga
     4141 aactggacaa gaacggccac acggcctacc tggagaacag cagcttcgat tacctggtgc
     4201 tgctgcaagg gtccggcgat gagctgctgc tgaacgagtc gcgccttagc gacgagcata
     4261 aggagcagcg caacaaaaag aacgttcctc tgctcatcac caacggcttc tcgatgctga
     4321 tgctcatctt tcatccttac tatcgcaaca gcttcgagtt tgcggcgatg cccgacgaga
     4381 tcatcgctgg gcatcgcacc gcgcaagtcc ggtttaccca tattccggga acacgcacgc
     4441 cggcggcgtt ggcagtgcga gggcgtgagt ttcctcttga attgaccggc accgcctgga
     4501 tcgagccgga aaccggacag gtggtgaagc tcaagatcgg gctggcgaaa gacatgagcg
     4561 atgtcgggtt gaaatcgctc actgcggaaa ttgactacga cccaattcac cttcccggct
     4621 ggacgcaagc ctatcgcttt ccggccgtgg ccaccgttga ggtggaaacc ttgcgccaac
     4681 gctggcggaa tgttcatcgc ttcagcaact acaaacgctt cctggttgat acctcggtca
     4741 cggttgttga cccggagcag aagaagtgag cgctcccgcc agcatcatga actatggctc
     4801 cgtgcagcac acccgggagc gcgcccaccg gatgcggctt cacctcgccg cgctaacagc
     4861 ggttgtcagc gttgttgcgc ttgccgtcta tggatggaac tactatctgc tggccgcggc
     4921 ggaacggcca ttctcgccca agcatgagtt gctgaagccc agcggctcca tcgccatcaa
     4981 gctggggatt gcaggcgtca tgttgttcat gatgatcttc ctctatccgc tgcgtaagcg
     5041 catcaagtgg ctgggcagga tcgggtcggc gagacattgg ctcgacttcc acgtgatcgc
     5101 tggactcacc gctcccgtgc tgatcgcatt tcatgcatcg ttcaagttca ggggtatcgc
     5161 cggaatcgcg ttctggatca tgctgtcggt cgcgatcagt ggcgttatcg gtaggtacat
     5221 ctatgcgcag ataccgcgtt ccttgtcttc tgcggagctc tcgcttaagg aattggccga
     5281 aatcgaagat gacctgtcga aagatctttc tgtccagggc ccgctctcgg cgcaggattt
     5341 cgcgtcgctg ctacaggtcc caactccagc ccaggtgcgg gagatgtcag cctaccgcgc
     5401 cattctgctg atgatggtgc tcgatctatg ccgtccattt tacatcgcgc ggctgcgtgt
     5461 ccgcgtgctg ggatggagcc gggtactgcc gtacttcgga ggattgatcc gcaccccgca
     5521 cgaggacctg gagcgggcca tccgcatagc acgaagcaag gccgcgctct cgaagaggat
     5581 tgccttcctc tcacgcacgc aacaggtatt ccacctgtgg cacgtcattc atcgcccctt
     5641 cagctactcg tttgcagtgc tggccctcat tcacatcgtg gtcgttattg cactgggatt
     5701 tgtgtaatca aggatggaga cattactaac attcctgctc gccacggccg ttaccctgtt
     5761 cttcgtgcgg ggttatttga aaaagttgac cacgccgaaa gcagttcgtg cagccactga
     5821 gcccaaggct cccaaggcaa tgcgagcgtg cccacggtgc ggccagtcca tggagaaagg
     5881 ctccgcattc tgccggcatt gtggcgcggc catggcgatg tggacggtac acgacgcggc
     5941 ggcgcaatcg cctggcgcag ggaacaaacc aagcgggact gccagaccga agcgcgatca
     6001 ccgaagcacc ctacggtgcg aagtagctgg agatgtcgag gatcagttgc gtgagccccg
     6061 acgcaaacgc atcgaccttt ccgttggtgc tgggcacgat tgcc
//
LOCUS       DASRKK010000483         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_233578, whole genome shotgun sequence.
ACCESSION   DASRKK010000483 DASRKK010000000
VERSION     DASRKK010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233578"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFC15_11260"
     CDS             <1..536
                     /locus_tag="VFC15_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10778.1"
                     /translation="YDGTAIDAQRAAEPLSELVAPQGVYFVAGNHEQFGDDSKYLHAI
                     AAAGVRVLSNEKVEVDGLQIIGVPYRNAALDSHLAAALRGIGLDPDRASVLLTHAPDH
                     PEVAEAAGVSLQLSGHTHLGQFIPWSWMARRFYRQFVYGLSQIGKMQVFTSSGAGTWG
                     PPLRLGSNPEIVMLEFQ"
     gene            655..1140
                     /locus_tag="VFC15_11265"
     CDS             655..1140
                     /locus_tag="VFC15_11265"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM10779.1"
                     /translation="MSAVLPPGTLAPDFTLPVTPDQKLSLLELRGHPVILAFYPADWS
                     PVCGDQMALYNEILPEFQKFGAELIGVSVDGVWCHRAFAEARHLHFPLLSDFEPKGAM
                     ARSYGAYRSGDGISERALFVIDKDGVIAWSYLSPIAVNPGADGILEALEGLTKPEKIH
                     G"
     gene            1133..1681
                     /locus_tag="VFC15_11270"
     CDS             1133..1681
                     /locus_tag="VFC15_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZM10780.1"
                     /translation="MANLRPPVTPGEHIQGAEDAPVTLVEYGDYECPHCGRAYSIIKR
                     VQKHFGKSLRFVFRNFPLSELHSHAESAAEAAEFAGARGKFWEMHDLLFENQERLSGD
                     LYLKLAEQLSLPIKALRKELEDGTYQTRVRADFSSGVRSGVNGTPTFFINGKRHDGPF
                     DYKALVLAIQEQIGRHGNAAVA"
     gene            1729..2208
                     /locus_tag="VFC15_11275"
     CDS             1729..2208
                     /locus_tag="VFC15_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10781.1"
                     /translation="MATKKTVKKKQAKSEQATRGKLAAMKKPSKKKAVGKGQPPKKQS
                     VSKSAHKKLTVKASSKKINNTRRGAVRRRSQTAKADSFSAQEAAARSAGQAGDLQGLS
                     HVAAADSESADELLEEGNAFEAGIVTGVEAADRATPSEVHTHEVLEDDVPEEYLNPD"
     gene            2587..3039
                     /locus_tag="VFC15_11280"
     CDS             2587..3039
                     /locus_tag="VFC15_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZM10782.1"
                     /translation="MSIVRTCKTCGRQNRIPAEHLADTGRCGACKSTLSPLAEPLEVD
                     SRLFDEIVEKARVPVLVDFWAVWCGPCRMAAPEVSRTAAETGGRAIVLKVDTERYPDL
                     ASRFNVRGIPNFAVFYRGHLIKQQAGLVDHEQMESWLESAAQSPVVRT"
BASE COUNT          765 a          856 c         1004 g          662 t
ORIGIN      
        1 tgtacgacgg gacggctatc gacgcgcaac gggcggcgga gccgttgagc gaactcgtcg
       61 cgccacaagg ggtgtatttc gttgccggaa atcatgaaca atttggggat gacagcaaat
      121 atctgcatgc catcgcggcc gcgggagttc gggtgctcag caatgaaaag gtcgaagtgg
      181 atggtctgca gattattggc gttccgtacc gcaacgcggc gctcgatagc catcttgcag
      241 cggcactccg cggtatcgga ttagatcccg atcgtgccag cgttctgttg acccatgcgc
      301 ccgaccatcc ggaggtcgcg gaggctgccg gggtctccct gcagctatcc ggacatacac
      361 atctggggca gttcataccc tggagttgga tggcccggcg gttttatcgg cagtttgtat
      421 atggcttaag ccagatcgga aagatgcagg ttttcacttc gagtggagcg gggacatggg
      481 gaccaccgct gcgcctggga tcgaaccccg agatcgtaat gcttgagttc cagtaaaggt
      541 caggatgttg gggccgaaat gccgccgcac aaaagttctt gtttgtgcac ctcggcagcg
      601 tcaatcgtca tcttacctcg aagatatcag tctgtacgag taagaggagg tcgtatgtcc
      661 gctgttctgc cgccgggcac actagcgccc gatttcacgc tgcctgtaac tcccgaccag
      721 aagctctcgc tcctggaatt acgcggacat ccggtgatct tggcttttta tcccgcggat
      781 tggagtccgg tgtgcggcga tcagatggcg ctctacaacg aaatcttgcc tgagttccag
      841 aagttcggcg cggagttaat cggagtgtcc gtggatggag tctggtgcca ccgggccttc
      901 gcagaagctc gacacttgca ttttcccctg ctctcggatt ttgaacccaa gggagccatg
      961 gctcgcagct atggagccta tcgaagtggg gacggaatct cggagcgcgc tctgttcgtc
     1021 attgacaaag acggagtcat cgcgtggagc tatctctcgc cgattgccgt gaatcccggc
     1081 gccgacggga ttctggaagc tctcgaggga ttgaccaagc cggagaagat ccatggctaa
     1141 tctcagacca cctgttacgc ccggagagca tatccagggc gcggaagatg caccagtgac
     1201 actagtcgaa tatggagatt acgagtgtcc acattgtggg cgtgcttact ccataatcaa
     1261 gcgcgtacaa aagcactttg gaaagagcct ccggtttgtg tttcggaact tcccgctgag
     1321 cgagttgcac tcgcacgccg aatccgccgc ggaggccgct gagtttgccg gagcacgcgg
     1381 aaaattctgg gaaatgcacg atcttctgtt cgaaaaccag gagcggctga gtggggacct
     1441 ttacttgaag cttgccgagc agctgagtct gcctattaaa gcgcttagaa aagagctgga
     1501 agacggtaca taccagactc gtgttcgcgc tgacttcagc agtggcgtcc gtagcggcgt
     1561 caatggcacc ccgacgtttt ttatcaacgg aaagcgtcat gatggaccct ttgattacaa
     1621 agcccttgtg ctggcaattc aggaacagat cggcaggcac ggaaatgctg cagtcgcgta
     1681 aagcggacgg ggttatcgct cgcgccccgc ggagaggaga ttgcacttat ggccaccaaa
     1741 aagacagtta agaagaaaca ggcgaagtcg gaacaagcga ctcgcggcaa actcgctgcg
     1801 atgaagaaac cgagcaagaa gaaggcggtt ggtaagggac agcctcccaa gaaacagtcg
     1861 gtgagcaagt ccgcccacaa aaagttaacc gtgaaggcgt ccagcaagaa gataaataac
     1921 acgcgtcggg gagctgttcg aaggcgaagt cagactgcga aagcggactc cttctcagca
     1981 caggaagcag cagcgcgttc ggccgggcag gcaggagatt tacagggatt gtcccacgtc
     2041 gcagctgccg attcggagag tgccgatgag ttgcttgagg aagggaacgc ctttgaggca
     2101 ggcatagtaa ccggcgttga agctgcggac cgtgcgactc cgagcgaagt ccacacgcat
     2161 gaagtgctgg aagatgacgt gccggaggag tatctgaatc cagactagtc gaaaaagatc
     2221 tgaggaatca gtcggcagag aggaatcgag gccgtgagtt cgaaaaggga ggccctggtt
     2281 cctgttcgag ccgagccgtg gtggagatta caccggccat tggaatctcg ttagatttgc
     2341 aaagccagag agccttgccg ccgttgatta ggagttcaca tgccagacac gggagaagat
     2401 cgagagatac agcgacgagt taacgctaaa gaagcagtac gcgtgtcgca atcccaaaaa
     2461 ctattacaga aatcgagaaa ggacgaggca actggccaac gccgcgccaa gaatggaaag
     2521 aggaagaacc cggaggatcg ataacggtca gcccaaggga agacggaacg tttggctgga
     2581 gagcttatgt ccattgttcg aacctgcaag acttgtggcc gacagaaccg aattcccgcc
     2641 gagcatcttg cggataccgg acgctgtgga gcttgcaaat caacactgag tcccttggct
     2701 gagccgctgg aggtagactc tcggttgttc gacgagatcg tcgaaaaggc tcgcgtgccg
     2761 gtgctggtcg atttctgggc cgtctggtgc ggtccttgcc ggatggcagc tcccgaagtt
     2821 tcgcgcactg ctgcggagac gggcggacga gccattgtac tcaaggtcga cactgagcgg
     2881 tatccagact tggcttcccg gttcaacgtg cgcggcattc caaacttcgc agttttttac
     2941 cgcggtcacc tcatcaagca gcaggcgggg ctagtcgacc atgagcaaat ggaaagttgg
     3001 ctagaatccg ctgctcaatc cccggtggtc cggacttgat gtgcgccaag agtccaatct
     3061 gagtgtggcc cgctgttgcg ccgccccaag gattcgcctt ccgccaaaca gcgaagaccg
     3121 ctgttcggtg gtcaggttcg agttgcccgt catccaggat caaccccgta cggagcacat
     3181 ctccgagatg gcgactattc gtccaagtgg tttccgtaac cgcgtgtcac gggagtgacc
     3241 aggtcaggcc ggcagacttc ccacaagaga acttctggct ctcgtcg
//
LOCUS       DASRKK010000484         5659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_724074, whole genome shotgun sequence.
ACCESSION   DASRKK010000484 DASRKK010000000
VERSION     DASRKK010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724074"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1755)
                     /locus_tag="VFC15_11285"
     CDS             complement(61..1755)
                     /locus_tag="VFC15_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HZM10783.1"
                     /translation="MQFSSRTFSPSSLTAILLLTLCLSSAQSWLFAQAPARAPQVAPA
                     PQQNILPYIKNAWNTLGRSMNDCSSISDPKFGAGATSVLYLPYGVTAPPAVAALSKCG
                     VKVENLPKKITGLGTIPVDQLNAHGLLYLPNKYVVPGGRFNEMYGWDSYFIIIGLLED
                     GERDYARGIIENFFYEIENYGAVLNANRAYYLTRSQPPFLTSMIMAQYAADKKAGKDD
                     KKWLARAYDYAVRDHDLWIKPPKLAGDTGLARYFDVGEGPVPDIADHPEYYAAIADWL
                     LKHPDEEAKYSYIAPTLEKGIGPELQVPVCDNKPCEEHHPVKLTADFYQGDRAMRESG
                     FDPSFRWGPFDGSTHHFAPVCLNSLLYKAEMDLAEMATILGHPEDAKKWRAAAETRKQ
                     LINKYLWNADKGMFFDWDFTTGKQSDYNYVTTFYPLWVGLATPEQAKAVMKNLGLFEH
                     EGGLAMSDQQTGVQWDLHYGWAPNHLIAVEGMRRNGFNSDADRVSVKFLDTIQSNFKR
                     TVTIREKYNVVTGSDEAPVLAGYHENLIGFGWTNGTFLALCHALPADQQKQVLEGK"
     gene            1913..3541
                     /locus_tag="VFC15_11290"
     CDS             1913..3541
                     /locus_tag="VFC15_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZM10784.1"
                     /translation="MNRLERIPAGFAEFKSSTSPAFCGLRPAAVTGLVLAILLLPWHS
                     AQGQTTEELQQQIQQMKQLYEQQIAALEGRIASLEQSNRAVAHATQENTVSVTDLHTE
                     VEKQVSAHEEPKLSRAQKTEIEQTELANTPRYDTVEDSEQVITELKQRVKAFEFHGYI
                     RSGQGLNGEGGQMVAFQAPGSGAKYRLGNEAETYGEMIFVNNWINPSRDPDKAWMKTE
                     VLVEANTTQASTYASTDQFRFREAFVEVGNVLDSNPSAQFWAGERYYRRLNIDINDFY
                     ILDTSGYGGGVENLDVKIGQMSVAYLGGAKDDLITSNGIYPKSLLDVRLYGIKTPVGQ
                     LGVWYDYSYSKGGTLADGTQVPSTGGWAIGLGHTRQEWLGGYNRISLQYGVGNAANFS
                     TSIDEPTVYLPNAHTFRFTDSAVIQPNDKFAVQPVFIYQSQTDGNPANGTSTWVSLGA
                     RPVWFFTDHVSLAFEAGFDHTNNGAGLYDGWLRKFTLAPQIGAGRKFFSRPVLRAFVT
                     YANWSQGLEGYIGGPAYKYKTTGWNFGVQGETWW"
     gene            complement(3547..4005)
                     /locus_tag="VFC15_11295"
     CDS             complement(3547..4005)
                     /locus_tag="VFC15_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10785.1"
                     /translation="MRASSKRLISVATFLLLTTISSQAQWVAVARAVSGRIQRMEQQG
                     ANGQGGYDVATVVIEANAKNVYETALQRLKAHAGALKVIREDARTRTVAFTNGVQTAS
                     LQANTLGDKLTQLVVASTLDPDQKSATPLVVNSIQQVCTELKVECTLQSD"
     gene            complement(4229..4480)
                     /locus_tag="VFC15_11300"
     CDS             complement(4229..4480)
                     /locus_tag="VFC15_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZM10786.1"
                     /translation="MAYTITDTCTKDELCVEACPTDCIHPKKDEETFEAATQLYVDPN
                     GCIDCGACVPVCPTNSIFPNDEVPADKAEFIDKNAAHYA"
     gene            4697..4966
                     /locus_tag="VFC15_11305"
     CDS             4697..4966
                     /locus_tag="VFC15_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10787.1"
                     /translation="MKEQLEQPIVVERVQTGVRMEKRLLKVLKGLAELKDMTLGDLLE
                     GIVLHAFEGKAPFSDNTLKEIEKLRSIYGLTLKAADSHQLKERRK"
     gene            4963..5520
                     /locus_tag="VFC15_11310"
     CDS             4963..5520
                     /locus_tag="VFC15_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10788.1"
                     /translation="MNSMHVLPLLALFAGAITSNCSQAQEVIKPVLAHTHQEFSFAVQ
                     APYEQVFPLFGALEEKKWAKGWEPEFVYPTPAHDQQGMVFTRKHGGMSSVWTCTAFDE
                     ATGHVQYVYVVDDAMVTLIDIHVVRAGPAETRVSVVYERTALQPEANEHVAHMAKGDA
                     GSGSEWAEAINGYLAKTLTGPANSK"
     gene            complement(5535..5624)
                     /locus_tag="VFC15_11315"
     CDS             complement(5535..5624)
                     /locus_tag="VFC15_11315"
                     /inference="COORDINATES: protein motif:HMM:NF044419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10789.1"
                     /translation="MATGVPVGSDIEYADEVTMQKAMEGRREL"
BASE COUNT         1169 a         1585 c         1727 g         1178 t
ORIGIN      
        1 cttcggtaga acggggcacc cagtgcctgg gagaacccgg gctcagttcg attcagcgct
       61 tcacttccct tccagcacct gcttctgctg gtcagccgga agcgcgtgac acagcgccag
      121 gaatgtcccg ttggtccatc cgaagccgat caggttctcg tggtagcccg cgagcaccgg
      181 cgcttcgtca ctaccggtca ccacgttgta cttctcgcgg atggtgacgg tccgcttgaa
      241 gttcgactgg atggtatcca agaatttcac cgacacgcga tcggcgtcgg agttgaaacc
      301 attacgccgc atcccctcga cggcgatcag gtgattgggc gcccatccgt aatgcaggtc
      361 ccactgcacg ccggtttgct ggtcgctcat ggccagtccg ccttcgtgct cgaagaggcc
      421 gaggttcttc atcaccgctt tggcctgctc gggcgtcgcc agtccaaccc acagcggata
      481 aaacgtggtc acgtagttgt agtcggattg cttgccggtg gtgaagtccc agtcgaagaa
      541 cattcccttg tcggcgttcc acagatattt gttgatgagt tgcttgcggg tttcggctgc
      601 ggcccgccac ttcttggcgt cctcgggatg cccgagtatg gtggccattt ctgcaaggtc
      661 catctcagcc ttgtagagca ggctgttgag gcacacggga gcgaagtggt gagtcgagcc
      721 gtcgaacggc ccccagcgga atgacgggtc gaatcccgac tcgcgcatgg cgcgatcgcc
      781 ctggtagaag tcggcggtga gttttacggg atgatgctct tcacacggct tgttgtcaca
      841 gaccgggact tgcaactccg ggccgattcc cttctcgagc gtaggcgcga tgtagctata
      901 cttcgcttcc tcgtcgggat gcttgagcag ccagtcggcg atagcggcgt agtactcggg
      961 atggtcggcg atgtcgggca ccgggccttc gcccacgtcg aagtagcgcg ccaggccagt
     1021 gtctcccgcg agcttgggag gcttgatcca gaggtcgtgg tcgcgcacgg cgtaatcgta
     1081 ggcgcgcgcg agccacttct tgtcatcctt gccggccttc ttgtcggctg cgtattgcgc
     1141 catgatcatc gaggtcagaa acggcggctg cgagcgcgtg aggtagtagg cgcgattggc
     1201 gttgagcact gcgccatagt tctcgatctc gtagaagaag ttctcgatga tgccgcgagc
     1261 gtagtcgcgc tctccgtctt cgagcaggcc aatgatgatg aagtagctgt cccagccgta
     1321 catctcgttg aagcggccac cgggcacgac gtacttgttg ggcagataga gcaggccgtg
     1381 ggcgtttagc tggtcgaccg gaatggtgcc gaggccggtg atcttcttgg gaaggttctc
     1441 gaccttcacg ccgcatttgc tgagagccgc tactgcggga ggagcggtca cgccatatgg
     1501 caggtagagc acagaggtcg cacccgcgcc gaacttggga tcgctgatcg acgagcaatc
     1561 gttcatggaa cgccccaacg tattccacgc attcttgatg tagggcagga tgttctgctg
     1621 cggcgcggga gcaacttgag gcgccctagc cggggcttgt gcaaacagcc aggattgcgc
     1681 cgacgagagg cagagtgtca gcaggagaat ggccgtcagg gagctcggcg agaaggttcg
     1741 actggagaat tgcatgacgc cggattatag ctgttgtctt cggcaatgag aaagctaggt
     1801 ctgtctcggt acccaatcaa ccaacgccaa cgtgacctct gagctagctc tccgccccgt
     1861 gcggtgaaat aatccgcgcg atatggttta gcccgattag gagcttgctt ccatgaaccg
     1921 tcttgaaaga attcccgccg gttttgccga gttcaaatcg tcaacctctc cggcgttctg
     1981 cggtttgcgt cctgcagccg tcaccgggtt ggtgctggca attctgctgc tcccctggca
     2041 ctccgcgcag gggcagacca ctgaggaatt acagcagcaa attcagcaga tgaagcagtt
     2101 gtatgaacag cagatcgcgg ccctcgaagg gcgcattgcc tcgctcgagc agtcgaaccg
     2161 cgcagttgcg cacgccacgc aggagaatac ggtttctgta accgacctgc atactgaagt
     2221 ggaaaaacaa gtgtccgctc acgaagaacc caaactcagt cgcgcgcaga agaccgagat
     2281 cgagcagacc gaactggcaa atactcctcg gtacgacacc gtggaggatt ccgagcaggt
     2341 cattacggaa cttaagcagc gagtcaaggc gttcgagttc catggttaca tacgctcggg
     2401 acaggggctg aacggcgagg gcggacagat ggtcgccttc caggcacccg gctccggggc
     2461 caaataccgg cttggtaacg aggcggagac ctatggcgaa atgatcttcg tcaacaactg
     2521 gatcaatccc agccgcgatc ctgacaaagc atggatgaag accgaggtgc tggtggaagc
     2581 caacacaacg caggcatcca cctatgcgag caccgatcag ttccgcttcc gcgaggcgtt
     2641 cgtcgaggtg ggcaacgtcc tggactcgaa cccgtcagcg caattctggg cgggcgagcg
     2701 ttattatcgg cggctcaata tcgacatcaa cgacttctac attctcgaca ccagcggcta
     2761 cggcggaggc gtggaaaatc tagacgtcaa gatcgggcag atgtcggtgg cctacctggg
     2821 cggggcgaaa gatgacttga tcaccagcaa cggtatttac ccgaagagcc tcctcgacgt
     2881 acggttgtac ggcatcaaga cgccagtggg acagcttggc gtctggtacg actactcgta
     2941 ctccaaaggt ggcacgctcg ccgacgggac ccaggttcct tcgaccggcg gctgggccat
     3001 tgggctgggc catacccgtc aggagtggct cggcggatac aaccgcatat cactgcaata
     3061 cggagtgggc aatgcggcga acttctcgac ctcgattgac gaaccgacgg tgtacctgcc
     3121 gaacgcgcat accttccggt ttaccgacag cgctgtcatt cagccgaacg ataaattcgc
     3181 agtgcagccg gtattcattt accagtcgca gaccgatggc aaccctgcga acggcaccag
     3241 cacgtgggtt tccttgggcg cacggccggt atggttcttc accgatcatg tctcgctggc
     3301 gttcgaggcg ggcttcgacc ataccaacaa cggagccgga ctctacgatg gatggctccg
     3361 caagttcact ctggcgccac agattggagc cggacgaaag ttcttcagcc gtccggtgct
     3421 gcgggctttc gtgacctacg ccaactggtc acaaggactc gagggttaca tcggaggccc
     3481 ggcgtataag tacaagacca ccggatggaa cttcggggtg cagggagaaa cctggtggta
     3541 gatggtctaa tcgctctgca gcgtgcactc gactttcaac tcggtgcaca cctgctggat
     3601 gctattcacc accagaggag tggcactctt ctgatccggg tccagtgtgg acgccaccac
     3661 caactgggta agtttgtcac ccagggtatt cgcctgcaag cttgccgtct gcactccatt
     3721 ggtaaaggcc accgtgcgcg tgcgggcgtc ctcccggatc acttttaacg cgccggcatg
     3781 cgccttcaat cgttgcagtg cagtttcgta cacgtttttg gcgttggcct caatgacgac
     3841 ggtcgcaaca tcatagccgc cctggccgtt ggcgccctgc tgttccatgc gctggatgcg
     3901 tccagatacc gcgcgcgcta ccgcgaccca ctgagcttgc gaagaaattg ttgtcagcaa
     3961 aagaaaagtg gccactgaga tgagacgctt actgcttgca cgcattttcg ctcctcgata
     4021 tgttttggac tggcggtgct tctcaccgtt ggagaaggcg tggtaagtct agctggaatt
     4081 ttacgggtgt caagcgaagc cggagtgctt ccaaagacga aggcacagct tcgcggctgt
     4141 gcctcggggg attctcaata gcaaatttag tgacaaacac gaccgcctgt ccgtgaggac
     4201 tcaggcgacg tggccttggc ccaaaacttt atgcgtagtg cgcggcgttc ttgtcgatga
     4261 actcggcctt gtccgctggg acttcgtcgt tggggaagat cgaattggtc gggcacaccg
     4321 gaacgcaggc gccgcagtcg atgcagccgt tggggtcaac gtacaactgg gtcgcggcct
     4381 cgaaagtctc ctcgtccttc ttggggtgga tacagtcggt gggacaagct tctacgcaga
     4441 gttcgtcctt ggtgcaggtg tcagtaattg tgtaagccat gattgcgatt ctcctgatct
     4501 tgtgtggcct gccagcctca tcggcagcag gtccggcgat tcttccgcca atttcactgt
     4561 acaaatttgg tgaccacggg gtcggtgata gccatcaccg acctgttgtg agcaagcgca
     4621 caagcgggtt tttgccgcgg ggctgcgttg caaggcggaa cttgacagta ttttggtaca
     4681 ttagtacatt cgtaccatga aagaacaact ggaacagcct atcgtcgtcg aacgcgtaca
     4741 aactggcgtc cgaatggaga agcgcctgct gaaggtcctc aagggtctgg ccgagttgaa
     4801 ggacatgacc ctgggcgacc tgctggaggg catcgtgctg catgctttcg aaggcaaagc
     4861 gcccttctct gacaacaccc tgaaagaaat tgagaaactg cgcagcattt acgggctgac
     4921 cctgaaggca gcggacagcc atcaactgaa ggagcgccgc aaatgaattc catgcatgtg
     4981 ttgccgctac tcgcgctttt cgcaggcgcg atcacatcga actgcagtca ggcgcaggaa
     5041 gtgatcaagc ctgtgctggc gcacacccat caggaattta gcttcgcagt ccaggcccca
     5101 tacgagcagg tgttccctct attcggagcg ttggaggaga agaagtgggc caaaggctgg
     5161 gaaccggaat ttgtctatcc gaccccggcg catgatcagc aagggatggt ttttacgaga
     5221 aaacacggcg gcatgtcgag tgtctggacc tgcactgcgt tcgacgaagc caccggacac
     5281 gtacagtacg tgtacgtggt ggatgacgca atggtcacgc tcattgacat tcacgtggta
     5341 agagctgggc cggcggagac tcgggtatcg gtagtttacg agcgcaccgc gctccagccg
     5401 gaggccaatg agcacgtggc ccacatggca aaaggagacg ccgggagcgg aagtgagtgg
     5461 gccgaggcga tcaacgggta cctcgcgaaa acgctgacgg gcccggccaa ttcaaaatag
     5521 aaaaagtgcc agtttcagag ttctcggcgg ccctccatcg ccttttgcat cgtgacctcg
     5581 tcggcgtact caatgtcgct gccgacggga acgccggtcg ctatgcgggt gaccttgacg
     5641 ccggaagact tgaggaggc
//
LOCUS       DASRKK010000485         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1114705, whole genome shotgun sequence.
ACCESSION   DASRKK010000485 DASRKK010000000
VERSION     DASRKK010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114705"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFC15_11320"
     CDS             complement(<1..678)
                     /locus_tag="VFC15_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZM10790.1"
                     /translation="MSSRSTLELRQQVGQLLIMGCVGTAMSARLRSMLGTLHPGGVIL
                     FQRNIEEAAQTHALLHDVQKAVSIPLFLCVDMEGGTVDRLRDVIAPAPAVADVAAAGS
                     KKLFRKHGRIIGDEVRALGFNTDFAPALDLQLEPSKSVLTSRTVSADPKETIQYAREF
                     LRGLSDCKVLGCGKHFPGLGEANLDSHNDLPLIDKAWKRLWNEDLLPYRELRRNLPFV
                     MVAHAAYP"
     gene            complement(761..1105)
                     /locus_tag="VFC15_11325"
     CDS             complement(761..1105)
                     /locus_tag="VFC15_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10791.1"
                     /translation="MGGKNGDPLRGVTVRLHYQNYSAEDDEHEVSLTTDDRGYVVFPA
                     QELTASRWKRLLTMLKSASAGVHASFGPHAWVSASGKGLEGVAVQGEYVMDWKGHPPE
                     IKSQITAEPMKK"
     gene            complement(1214..>2573)
                     /gene="mutS"
                     /locus_tag="VFC15_11330"
     CDS             complement(1214..>2573)
                     /gene="mutS"
                     /locus_tag="VFC15_11330"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZM10792.1"
                     /translation="DEPPLTLADGGVIRSGVDAGLDELRDLSRNSKQYIAQIEQRERQ
                     RTGIGSLKVKFNSVFGYYLEISKANLHLAPREYERKQTLVNAERFTTPELKEYEVKVL
                     DAQEKIVEIERRLFAELRTAIAAEARRIRQTALALAEIDVLAAFAALAANRNYCRPLF
                     RDGADIEIVEGRHPVIEQMEIPGAANRFVPNDLYLNGTTHSILIITGPNMGGKSTYLR
                     QAALIVLMAQMGSFVPARSASMGLVDRIFTRIGAADNLARGRSTFMVEMTETAAILNT
                     ATARSLILLDEVGRGTSTYDGLAIAWAAIEYIHANTRAKTLFATHYHELTELADRLSG
                     VKNYHVSVKESGGGIVFLRKVEEGAADKSYGIEVAKLAGLPADVVNRAREVLAEHESA
                     ERSAVKHLAHDETQETAPLQLTIFTPLSQKIVDRIKETDLNRISPMDALTLLHELKKQ
                     ME"
BASE COUNT          519 a          764 c          748 g          542 t
ORIGIN      
        1 gggataagcc gcgtgcgcca ccatcacgaa tggcaaattt cgtcgcagct cacgataggg
       61 aagcaggtct tcattccaga ggcgcttcca agccttgtca atcaatggca ggtcgttgtg
      121 cgagtcgaga ttggcttccc ccagacccgg aaagtgcttg ccgcagccaa gaaccttaca
      181 gtcgctgagt ccgcgcagaa actcgcgcgc gtattggatc gtctctttgg ggtcagccga
      241 aaccgttcgc gaggtcagca cacttttcga tggctcaagc tgaaggtcga gcgccggcgc
      301 gaaatcggtg ttgaacccta acgcacgcac ttcgtcgccg atgatgcggc catgtttgcg
      361 gaagagcttc ttcgaaccag cggctgcgac gtcggcaacg gctggcgctg gcgcgatcac
      421 gtcgcgcagg cgatcgaccg ttccgccttc catgtccacg cacagaaaca gaggaatgga
      481 aaccgccttc tgcacatcgt gcagcagagc gtgagtttgg gcggcctcct cgatgttgcg
      541 ctggaagagg atgacaccgc ccggatgcag tgtgcccaac atggaccgca gacgcgccga
      601 catcgcggtg cccacgcagc ccatgatcag caactggccg acctgctggc gaagttcgag
      661 tgtgcttctg gaagacatag ggaataagaa agtgtaacgc tagcaggtca ggaattcagc
      721 tatacaggat ggtacagccc gtgcgagtta cagatagcgg ctatttcttc atgggttcag
      781 ctgtgatttg agactttatc tccggaggat gacccttcca gtccatgacg tattcgcctt
      841 gaacagcgac cccttcgagt ccttttcccg aagcggaaac ccaggcatgg ggaccaaaac
      901 tagcatgtac gcccgcgctt gccgacttga gcattgtcaa caggcgtttc caacgagatg
      961 cagtcaactc ctgagcgggg aaaacgacat aacctcgatc atcagtagtc agtgagactt
     1021 catgttcgtc gtcttcggct gaataatttt gataatgcag gcgaacagtg acgcctctga
     1081 gcggatcgcc gttcttacca cccaaacctc ccaacgagga cagtttagtg tagtgaccgg
     1141 ccacaagaga aaggcgcaaa acgcgacaat caccagcgtt gtgacgacgg cttgtctgga
     1201 caatcccccc caatcactcc atctgcttct tcagttcatg cagcagggtc agcgcgtcca
     1261 tggggctaat gcggttgagg tccgtctcct tgatgcgatc cacgatcttc tgcgaaagcg
     1321 gggtgaagat ggttagctgc aacggtgcgg tctcctgcgt ttcatcgtgt gccaagtgct
     1381 tgaccgcact ccgctcggcg ctctcatgct cggccagcac ctcacgcgcg cgattcacca
     1441 cgtcagcggg caggccagcc agcttggcga cttcaattcc gtagctcttg tcggctgcgc
     1501 cttcttccac cttgcggagg aaaacaattc cgccgccgct ctccttcacc gagacatgat
     1561 agttctttac cccactcagc cggtcggcca gctccgtcag ctcgtgataa tgcgtggcga
     1621 agagcgtctt agcgcgggtg ttggcgtgga tatattcgat agcggcccac gcgatggcca
     1681 acccatcata ggttgaagtt ccgcgcccta cctcgtcgag caggatcagg gagcgcgcag
     1741 tcgcggtgtt gaggattgct gcggtctcgg tcatctccac catgaaggtc gagcgcccac
     1801 gagccaggtt atcggctgcg ccgatgcgtg tgaagatgcg gtccaccagt cccatgcttg
     1861 ccgaccgcgc ggggacgaac gatcccatct gtgccatcag cacgatgagc gcagcttgcc
     1921 gcaagtacgt ggatttgcct cccatgttcg gcccggtaat gatcaggatc gaatgagttg
     1981 tgccgttcag atagagatcg ttggggacga agcgattggc ggcaccgggg atctccatct
     2041 gctcgatcac agggtgacgc ccttcaacaa tttcgatgtc cgcgccatct cgaaacagcg
     2101 gacggcagta gttgcgattg gcggcgagcg ccgcgaacgc agcgagcaca tcgatttccg
     2161 ccagcgccag cgcggtctgc cggatgcgtc gcgcctcggc ggcaattgcg gtgcgcaact
     2221 ccgcaaacag gcgccgctcg atctcgacta tcttctcctg ggcgtcgagc accttgacct
     2281 cgtactcctt caactctggc gtggtaaagc gttcggcgtt gaccagggtc tgcttgcgtt
     2341 cgtactccct aggcgcgagg tgcaggttcg ccttggagat ctcgaggtaa tagccgaaga
     2401 cgctgttgaa cttgaccttc agcgaaccga tgccggtgcg ctggcgttcg cgctgctcga
     2461 tctgtgcgat gtactgtttg ctgttacggc tcaggtcgcg aagttcgtcg agtcccgcat
     2521 cgactcccga ccggatgacc ccgccgtccg ccagcgtaag cggaggctca tct
//
LOCUS       DASRKK010000486         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1017406, whole genome shotgun sequence.
ACCESSION   DASRKK010000486 DASRKK010000000
VERSION     DASRKK010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017406"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="aroF"
                     /locus_tag="VFC15_11335"
     CDS             complement(<1..795)
                     /gene="aroF"
                     /locus_tag="VFC15_11335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZM10793.1"
                     /translation="MLIVMKAHASEEQVERVCEKIESLGFRAHAMPGAQRTAIGVTGN
                     QTEVETGTFDEMEGVQEVIRVSKPYKLVSRDLKEEDTVVRFPNSGATFGGEEVAVIAG
                     PCAIESRDQAFATAECVARAGARFFRGGAYKPRTSPYTFQGLAEEGLKILAEVRDRFG
                     LLIVTEAIDNESLELVEKYADVIQIGARNMQNYSLLKRAGRAHKPVLLKRGMSATLEE
                     LLMAAEYIMSEGNYNVILCERGVRTFADHTRNTLDLSIVPAVQRLSH"
     gene            1237..1638
                     /locus_tag="VFC15_11340"
     CDS             1237..1638
                     /locus_tag="VFC15_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM10794.1"
                     /translation="MFSTKQRQNLIRNPEELWRYVAALAHNKNIHVLAAGGTANHLHV
                     LMLLPQTITLAAAMQEIKANSSRWLRETSAEFQWQEGYGAFSVSQSQRQVVVDYIASQ
                     AQHHQKHSYEEEFLALLKNSGVQYEPRFIFG"
     gene            2013..>2504
                     /locus_tag="VFC15_11345"
     CDS             2013..>2504
                     /locus_tag="VFC15_11345"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZM10795.1"
                     /translation="MADSKTEEPNTKPQSSAQTEVVNGGAAGRAERYDPQAIEQKWFD
                     RWAQNPDLYRAEPPSSSRKKYYVLEMLPYPSGVLHMGHVRNYAIGDALARYMWMQGYN
                     VLHPMGWDAFGLPAENAAIQNQTPPRQWTLHNIASMKVQMKRLGFAYDWSKELATCLP
                     EYYR"
BASE COUNT          531 a          752 c          724 g          497 t
ORIGIN      
        1 gtggctcaaa cgctgcaccg ccggcacgat gctgaggtcg agcgtgttgc gcgtatggtc
       61 ggcgaaggtg cgcacgccgc gctcgcacag aatcacgttg tagttgccct cgctcatgat
      121 gtattcggca gccatcagca attcttccag cgttgccgac atgccgcgct tcagcagcac
      181 aggcttatgt gccctgcccg cgcgcttcaa cagcgaataa ttctgcatgt tgcgcgcgcc
      241 aatctggatg acatcagcgt acttctccac cagttcgaga gattcgttat cgatggcctc
      301 ggtgacgatc agcaggccga agcggtcgcg gacttccgct aggatcttca ggccctcctc
      361 cgccagacct tgaaacgtgt agggagaagt gcgcggtttg taggcgccgc cgcggaagaa
      421 gcgggcaccg gcgcgggcga cgcactcggc ggtagcgaac gcctgatcac gggattcgat
      481 agcgcagggt ccggcgatga ccgctacctc ttctccgcca aaggtcgcgc cagaattggg
      541 gaagcgcacc accgtgtctt cttccttcaa atcgcggctg acaagcttgt aaggcttgct
      601 gacgcggatg acctcctgca ccccttccat ctcgtcgaag gtgccggtct cgacctccgt
      661 ctggttgccg gtaacaccga tggcggtgcg ctgcgctccg ggcatggcat gcgcgcgaaa
      721 gcccagtgat tcgatctttt cgcatacgcg ctccacttgc tcttcggagg cgtgcgcctt
      781 catcacgata agcatcaggg accccttgtt tcgtgcccca tagtgtatcg cggaaggcgc
      841 ggcgctggga acgaaaaggc agcgtgtggg gggttgaagg cgctggtcgc cgatgcgaag
      901 gcattgccaa gaggcaaacg cagatgaaga cgcttgccgc gcctaatgca gatttcctcg
      961 catgggcagc gtcaactgtt cgttttgatc ggtagccagt acggccatcc atcgacgacc
     1021 ctgacggatt cagcgacaac ggcgagtccg cgaagcggac gctatagtct agcccagcgt
     1081 gaaacgctgg gtatcggatg ttttagaatt ttgagccccg ggaaggggcg acagccttgc
     1141 acgtcaggcg gtgcgtggcg tcactgcatt aatggccaat gattttctgt tctccaattt
     1201 atgtcgcata cccacgctca taacctctta cattgcgtgt tcagtaccaa acaacggcag
     1261 aacctgatcc gcaacccgga ggaactctgg cgctacgtgg ccgcacttgc ccacaacaag
     1321 aacatccatg ttctggccgc aggtggtacc gcgaaccatc ttcatgtgct catgctcttg
     1381 ccgcagacca tcacactggc ggcggcgatg caggagatca aggcgaacag ctcccgctgg
     1441 ctgcgcgaga cctcggccga atttcaatgg caggaaggtt atggagcctt cagcgtcagc
     1501 cagtcgcagc ggcaagtggt cgtggattac atcgcaagtc aggcgcaaca ccaccagaag
     1561 cacagttatg aagaagaatt cctcgccctg ttgaagaatt caggagtgca atacgaacca
     1621 cggttcatct tcggttgagg aaggctgtcg tgcctaacgg cactcgggtg taattgtcat
     1681 cgcgtaccca gcgtttcacg ctgggtgcag ggcgtttgga attctgagcc ccggaagggg
     1741 cgacaaccct gaacgtcagg cggtgcgcag ggccaccaca ttttgtacca cgactggtcc
     1801 gcggttgaga agggctatcg tgcctacggc actcagattc ggcatccgtc cgttacccag
     1861 cgtttcacgc tgggctagac tatagcgtcc gcttcgcgga ctcgcattaa gatcagacag
     1921 caatctctcc agcgatgggt atagttgtag cggcattcca ttcaccccac attgtaaaat
     1981 caaggtttac cgcaagcgca tcaaaaacac ctatggccga ctccaaaacc gaagagccga
     2041 atacgaagcc gcaatcaagc gcccaaactg aagtcgtcaa cggtggcgcc gccggccgcg
     2101 ccgagcgcta tgacccgcag gcgatcgagc agaaatggtt cgatcgctgg gcgcagaatc
     2161 ccgatctcta tcgcgccgaa ccgccgagca gctcccgcaa aaaatattac gtgctggaga
     2221 tgctgcccta tccctccggc gtgctgcaca tgggacacgt gcgcaactac gccatcggcg
     2281 atgccttggc gcgctacatg tggatgcagg gctacaacgt gcttcatccc atgggctggg
     2341 atgcgttcgg acttcccgcc gaaaacgcgg ccatccagaa ccagactccg ccgcgccaat
     2401 ggacgctgca caacattgcc agcatgaagg tgcagatgaa gcgcctcggc tttgcctacg
     2461 actggtcgaa ggaactcgcc acttgcctgc cggagtacta ccgc
//
LOCUS       DASRKK010000487         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_333214, whole genome shotgun sequence.
ACCESSION   DASRKK010000487 DASRKK010000000
VERSION     DASRKK010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333214"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFC15_11350"
     CDS             complement(<1..716)
                     /locus_tag="VFC15_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10796.1"
                     /translation="MLIEIEHVEAIFRYPVKSMGGDRLEVADLGWHGLDGDRRLAFRR
                     MDDRSGFPWLTASKLPDLLLFAPHRREDGAEGDLPTHIRTPDGQAMPVFGEDLAAKVG
                     RRYGASVQIMQLKHGIFDEASISVIALDTVREIGRLAGRNLDVRRFRPNVVVRLLRPG
                     PFQEDEWLGGVLSFGEGDDAPAITVTMRDVRCSMVNLDPDSASPAPEVLKAVVRVHQN
                     PAGIYGAVTRIGRLAVGQTIL"
     gene            1141..2187
                     /locus_tag="VFC15_11355"
     CDS             1141..2187
                     /locus_tag="VFC15_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10797.1"
                     /translation="MKPRFLLLPVLILNMVAIAIALHAASDQTANYTTRTLALPDHGQ
                     GNVTMDYIAYDPKTGYVWIPAINLGSVYVVDTGNGSVREISGFATNEVELGGRKRRQG
                     PSGVSVGDGVVYIGDRADSSVCAINEQTLARKTCGHIDSTPDGVVYVAPTKEVWVTAP
                     RDKSVRILDSQTLAQKEKLTFEGRPEGYAVDAKRGLLYMNYEDKDLTTAIDLKTRKTV
                     AKWPSSCGEDGPHGLSVDQDTGFLFVACSTQVEVLDAGHNGEKLSSLDTGDGVDDLDY
                     SPATHLLYVGAAKDARLTVARVDAAGKLNLIAQVPTHEGARNGVVTKDGTVYLAHSQL
                     GQLAGLIVVSPSRK"
     gene            complement(2855..3427)
                     /locus_tag="VFC15_11360"
     CDS             complement(2855..3427)
                     /locus_tag="VFC15_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM10798.1"
                     /translation="MGIQFILFMGLDVGIALLMLRQSGLWQRLRAAPLSRTMLLGSRA
                     VSASLIAGFLLLVLFGFARVVFGVHIRGSFAGFLGVCAAFSLMTAAFGLMVAALGETV
                     EATRGYSIMATLIMVMLGGAWVPTFIFPKWLQKLTVIIPTRWAMDGLDGMTWRGLGFS
                     SALAPIAVLLLFTLLFGLIAVMRFRWRTEG"
     gene            complement(3373..>3945)
                     /locus_tag="VFC15_11365"
     CDS             complement(3373..>3945)
                     /locus_tag="VFC15_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10799.1"
                     /translation="QLSSDKNLDVKPSTLEAGREAVRKGKATAAIVIPKDFGRDAGHA
                     LFTGATKPELGVLYDPSHNVELGMVKGILSGAVMQAVSKEMFTGKSGREMVNESLAQV
                     ENNPQMPAENRKALRDLLGGVKEFNEQQDREGASGQGELSGGLSMPYQTREQASPRDK
                     TSSITGTPIRSEAWAFSSSFSWDLMSESRC"
BASE COUNT          847 a         1171 c         1139 g          788 t
ORIGIN      
        1 agtatggtct gtccgaccgc cagtcgaccg atgcgagtaa ccgcgccata gattcccgcg
       61 gggttctggt gcacacgaac gaccgccttc agcacttctg gcgccggact tgctgagtcg
      121 ggatcaaggt tcaccataga gcagcgcacg tcgcgcatcg tgacggtgat ggcgggggcg
      181 tcgtccccct cgccgaatga gagcacaccg cccaaccact cgtcctcctg gaagggaccc
      241 ggtcgcagca aacgaaccac aacattcggg cgaaatcgtc gcacgtccag gttccgccct
      301 gcgagtcgtc cgatctcgcg caccgtatct agggcgatca cggagatgct cgcttcatcg
      361 aagatgccgt gcttcaactg cattatctgc acggaagctc cgtagcggcg tccgaccttc
      421 gcggccaagt cctcgccgaa gacaggcatc gcttggccat ccggcgtgcg aatatgcgta
      481 ggaaggtctc cctcagcacc gtcttcacgg cggtgcggag caaacaggag caggtcggga
      541 agcttgcttg ctgttagcca cgggaagccg ctgcggtcat ccatccgccg gaatgccagg
      601 cgtcggtcac catcgagacc gtgccagccg aggtcggcga cctcgagtcg gtcgccgccc
      661 atggacttca cgggataccg gaagatcgcc tccacatgtt cgatttctat gagcatgctt
      721 ggtccgagtc cgcccaacag atagggttgt ttccgtaggc gcagcgccga ggacgtgagt
      781 gcatctcgga aatcggtgcg cctacggaaa aagcctgttg gactaagccg gggtattcgc
      841 cgcttcagtt cagacttgaa tatagataaa tctgacctga cctgactggg ttaggggaca
      901 ggagctgtgg agttttgtgg caagcttaga ggaattgacc ggcagtaacg gtcaccccat
      961 tatcagacgt ccgactcccg ctgcacagag atgatcgacg cgacaagttc gcaccgctgc
     1021 atcctcggga agcttggcgg tggtggctat tcaagcggcg accggtccgg gcaggcagtc
     1081 cggctggcaa gtgttctcat caagtgtccg catctccaag gtggtgctaa gattccagcc
     1141 atgaaaccgc gattcctctt gcttccggtc ctcatactga acatggttgc aatcgccatt
     1201 gcattgcatg ctgccagcga ccaaaccgcg aactacacga ctcgtaccct cgcgcttccc
     1261 gaccacggcc agggaaacgt caccatggat tacatcgcct atgatcccaa gacgggatat
     1321 gtctggattc ccgcaattaa cctaggctcg gtgtatgtag tggacaccgg caatggttcc
     1381 gttcgcgaga tctcgggctt tgccacaaac gaagttgaac tgggcggccg aaagcgccgt
     1441 caggggccca gcggcgtatc ggttggcgat ggagtggtat acatcggcga ccgcgccgat
     1501 tccagcgtgt gcgccatcaa tgaacagacg cttgcgcgca agacctgtgg acacatcgat
     1561 tccacccccg acggcgtagt ttatgtggcg cccacgaagg aagtctgggt caccgcgccg
     1621 cgggacaagt ctgtgcgtat tctggacagc cagaccttgg ctcagaaaga gaaactgacc
     1681 ttcgaggggc ggcccgaagg atatgcagtc gatgccaagc gcgggctact ctacatgaat
     1741 tacgaggaca aagaccttac gactgccatt gatctcaaga cgcgcaaaac ggttgcgaag
     1801 tggccgtcat catgtggcga agacggcccg cacggtctat ccgtggatca agacactggg
     1861 ttcctcttcg ttgcctgcag cacgcaggtg gaggttttgg atgcgggaca caacggggag
     1921 aaattatcat ccctcgatac cggcgacggc gtggatgatc tcgactactc gcctgccact
     1981 cacctgctgt atgtaggcgc tgccaaggat gcgcggctta cggtcgcccg agttgacgct
     2041 gccggcaagc tcaacttgat tgcacaagta ccgacgcacg agggcgctcg gaatggagtc
     2101 gttaccaagg acggtacggt ctatctcgcg cactcgcaac tgggtcagtt ggcagggctg
     2161 atcgtggtat cgccgagtcg aaaatagccc cgagccaaac ggggacgacg ccagaatcta
     2221 aagcgccaca atcaatccag gcatggctta cccacaaaat aactgattgg agtcgagcaa
     2281 cggagtattg ggaagaaatc tacccagggt taaccagcgc aggaatggag acttcgtcgg
     2341 cccggagaat ggcgacaact ttttcgatgt aatgcgcgaa ccggtcaact tgccagaagg
     2401 gcgtctatgc tctatgtgct gaaacttccc acaacaagac ttcgggcccg tgtcgttgac
     2461 tggctgacga gatgaattgc ctttggttct tggaacacga cagtgtggat gttagtcgga
     2521 tagaacggct ggtggccgcg cgcaaatcgc ccagcaagag gccccaaaaa acgtaaaggg
     2581 cgctacagta attccagcat ccgatcacgc cattacccgg cttgccgacc gctctaattc
     2641 gtacttcaat cgatccctac ctcgccttac gcttatttcc aagggtttgg gcgggtccct
     2701 agcagtgatt gacaccgtag aggtcaggag ttcgagtctc ctcttgccta ccatttcttt
     2761 caatcactta gcttctgttt ctagagcgga atgacgtttc cgatcgaccg tgccgcgttc
     2821 cttgaacctc ggccgaacgg agcggtaccc attgctatcc ctccgtcctc cagcggaatc
     2881 gcattaccgc tatcaaaccg aacaacagcg tgaagagcag caatacagcg atgggcgcaa
     2941 gtgccgagga gaatcccaag ccgcgccagg tcatgccgtc caacccatcc atcgcccacc
     3001 gggtcggtat gatcacggtt agtttctgga gccactttgg gaagatgaaa gttggcaccc
     3061 aggcaccgcc cagcatcacc atgatgagcg tcgccatgat ggagtagccg cgtgtggctt
     3121 cgaccgtctc accgagggcg gcgaccatga ggccgaaggc ggcggtcatc aatgagaatg
     3181 ctgcgcacac tcccaggaaa cccgcgaagc tgccgcggat gtggacgcca aacacgaccc
     3241 gggcgaagcc gaagagcacc agcagaagaa atcccgcgat cagggaggcg ctcaccgccc
     3301 ggctgcccag cagcatggtt cgggaaagtg gcgcggcccg gaggcgctgc cagagcccgc
     3361 tctgacgcag catcagcagc gcgattccga catcaagtcc catgaaaagg atgaactgaa
     3421 tgcccatgcc tccgaacgaa tgggcgtacc cgttatactc gacgttttgt cccgaggtga
     3481 tgcctgctcc cgcgtttggt agggcatgct caggccgccg gataactctc cttgcccgga
     3541 cgcaccctcg cggtcctgtt gttcgttgaa ttccttcacg ccgccgagca gatcgcgaag
     3601 agccttccgg ttttcagccg gcatttgagg attgttctcc acttgagcga gcgactcgtt
     3661 caccatttcg cgtccgcttt taccagtgaa catctcctta ctgacggcct gcatgaccgc
     3721 accactcagg atgcccttca ccattccgag ttccacgtta tgggaagggt cgtagagcac
     3781 gccgagttcg ggtttggtcg cgcccgtaaa tagcgcgtgt ccagcgtccc tgccgaaatc
     3841 cttggggatg acaatcgcgg cggtcgcttt ccctttgcga accgcctcgc gaccagcctc
     3901 gagcgtggat ggctttacgt cgaggttctt atcgctgctg agctg
//
LOCUS       DASRKK010000488         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_919206, whole genome shotgun sequence.
ACCESSION   DASRKK010000488 DASRKK010000000
VERSION     DASRKK010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919206"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFC15_11370"
     CDS             complement(<1..548)
                     /locus_tag="VFC15_11370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM10800.1"
                     /translation="MTTGYLLAREGKSVVILDKNQIGTGETAHTTAHLSNVLDTSYRE
                     IEGLHGTKDAQLVAQSHTAAIAQIESIVAEEKIDCDFERLDGYLFFADGDSQEKLQQE
                     WQAAKHAGLQVRKLKQAPSDLKLGPCLRFPQQAQFHPLKYLAGLAHAIKRLDGRLFGE
                     TEAKEIKGGKTVRIRTKGGAIVS"
     gene            complement(1116..2348)
                     /locus_tag="VFC15_11375"
     CDS             complement(1116..2348)
                     /locus_tag="VFC15_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM10801.1"
                     /translation="MLDNVRLELKPTENAPVHTQGVCGPAAAPPFAKVLARRRINTHD
                     GPARIVPSAASVEKWTEFRKTRSKRIACKIGFALLAIALFQMASVVGEQTTGSNELKA
                     LGIALEEYPYPWPAHFLPLMIEGQDLRMAYMDVPPSGPANGRAVVLMHGKNFGGYYWR
                     DVAQALAAAGYRVIVPDQVGWGKSSKPDIRYSFQLLAANTAHLLDTLGIKKVAVVGHS
                     TGGMLAVRFALMYPERVTQLVLEDPIGLEDYRVNIPPQPDETLYRAELADTDAAKIRA
                     FYAHYFANPQPKIYEPLAEVQIRVTLSGEYPRWAKASAIAYQMIYQQPVRYEYRLLQP
                     PTLLIVGEKDHTVPLASYAAAEARAKMGDFVELGRAAVKEMPHGTLVVVSDCGHIPHI
                     EKSREFQQAVMGFLRGSE"
     gene            complement(2403..>2934)
                     /locus_tag="VFC15_11380"
     CDS             complement(2403..>2934)
                     /locus_tag="VFC15_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10802.1"
                     /translation="LKTVEDFTEFEQRAAYFDGDPVIATKKGVNVLERGSQVHFMAEF
                     QELLDFPPAPKQGWDGKLDPAKATPAEMHGQEVFFGKGQCSSCHQPPYYTDNTMHNLQ
                     AERFYQQHIVNGMMMADDGPIKTFPLRGIKDTPPYLHDGRLLTLDDTVEYFNLILGRN
                     LTDQEKKDLVAFLRAL"
BASE COUNT          606 a          851 c          779 g          698 t
ORIGIN      
        1 gaaacaatcg cgcctccttt cgttcttatc ctgaccgttt taccgccttt tatttctttc
       61 gcttccgttt cgccgaacag tcgaccgtcc aatcgtttga tcgcgtgcgc aagacccgct
      121 agatacttca agggatggaa ctgtgcctgt tgtggaaagc ggagacacgg tccaagtttg
      181 agatcggacg gagcttgctt cagttttctt acttgcagcc cagcatgttt tgccgcttgc
      241 cattcctgtt gtaacttttc ttgagagtct ccatcagcga aaaacagata tccatctaag
      301 cgttcaaaat cacaatcgat tttctcttca gccacgattg attcaatttg ggcaatcgct
      361 gcggtatgac tctgcgcgac caattgagcg tctttcgtgc cgtgaagacc ttcgatttca
      421 cgatagctag tatctaaaac atttgaaaga tgcgcggtag tgtgcgcagt ttcgcctgtt
      481 cctatctggt ttttgtcgag gattactacg gactttcctt cgcgcgccag cagatagcct
      541 gtggtcatgc ccgcgattcc ggcgccgacg atacagacat cgacatggat attctcgtca
      601 agcgagggcg ctgcagacct cttcgtcgaa gcggaccaaa ttgagacgct ttctcgggaa
      661 cccgtaggat gcttcgacgt aggcttttgg gcgcttcgtc tttggctaag aactcttgga
      721 gccatcccta ttctccgatc aatcgcttgt tagccggaag ttccatttgg aatccgagca
      781 gcagcattct ataccgattc gcctcgtccc ggagtttttt tgatcctcgc tggcggtaaa
      841 aaaaccgatg cagcgattgc gtcacgatgg ttgcaaagaa atgctagcca gccccgcgct
      901 ggatcaagac actgggtcga ccgcttaatc cagcgcgaca ccgttcgttc ctagaagtcg
      961 aaacccagct tgagcacctc gccgtgaatg cgtgatgctc ctcaggtagg attctcagcg
     1021 accatgcgaa agatcagctc gcgtacttta ggagcaggaa gccgagccga ccgtcatcgg
     1081 cgggcgcatc tatgcctagc ctccgactct ggtccctact cgctgccgcg cagaaagccc
     1141 atcaccgcct gctggaattc ccgcgacttt tcaatgtgcg ggatgtgccc gcagtccgaa
     1201 accacgacga gcgtgccgtg tggcatctct ttcactgcgg cacgtccgag ttcaacgaaa
     1261 tctcccatct tcgcgcgcgc ctcggcagcg gcgtagctcg caagcggcac cgtgtgatcc
     1321 ttttcgccga cgatcaacaa cgtcggcggc tggagcagcc ggtattcgta tcgaaccggc
     1381 tgctggtaga tcatctgata tgcgatggca gaagcctttg cccagcgcgg gtattcgccg
     1441 ctcagcgtca cgcggatttg tacctccgcg agcggttcgt agatcttcgg ctgcggattc
     1501 gcgaagtaat gtgcataaaa cgcgcgaatc tttgctgcgt ctgtatctgc gagttcagct
     1561 cgatagagcg tctcatccgg ctgcggcgga atattcacgc gataatcttc gaggccaatc
     1621 ggatcttcga ggacgagctg cgtcactcgc tccggataca tcagcgcgaa ccgaaccgcg
     1681 agcatccctc cggtcgaatg cccgaccacc gcaaccttct tgattccaag tgtatcgagt
     1741 agatgcgccg tgttcgccgc cagaagctga aagctgtagc gaatatccgg cttcgaggac
     1801 ttgccccacc cgacctgatc gggcacgatc acgcgatagc ccgcagcggc gagggcctga
     1861 gccacgtcgc gccagtaata tccgccgaag ttcttgccgt gcatcagcac aaccgcccgg
     1921 ccattcgccg gtccggacgg aggcacatcc atatatgcca tccgaaggtc ctgcccctcg
     1981 atcatgagcg gcaaaaaatg cgccggccat ggatagggat actcttcgag cgcgatgccg
     2041 agcgccttaa gttcgtttga ccccgttgtc tgttccccga caacgcttgc catctggaat
     2101 aacgcaatcg ccagaagcgc aaagccaatc ttgcaagcaa ttcgcttcga tctcgtcttg
     2161 cggaactccg tccatttctc aactgaagcg gcggatggca cgatgcgagc gggcccatca
     2221 tgggtattga ttctacggcg ggcaagaact ttggcgaagg gcggtgccgc agccggtcca
     2281 caaacgcctt gcgtgtgaac gggcgcattt tccgtgggct ttagctccag ccggacatta
     2341 tcaagcaaag gtttcttgag acctcttagc gcagaaattc caggggcgca ggcaaccgcg
     2401 cttcacagtg cccgcaagaa ggcgacaagg tctttcttct cctggtctgt caagtttcgc
     2461 ccgagaataa ggttgaaata ctctaccgtg tcgtccaagg tgagtaatct cccgtcgtgc
     2521 aagtacgggg gcgtgtcctt aatgccgcgc agggggaatg tcttgatcgg tccgtcgtct
     2581 gccatcatca ttccatttac gatgtgctgc tggtagaagc gctccgcctg caaattgtgc
     2641 atggtgttgt cggtatagta gggcggctgg tggcatgacg aacactgtcc tttcccgaag
     2701 aaaacttcct gcccgtgcat ctcagcaggt gttgcctttg ccggatccag tttaccgtcc
     2761 cacccctgct ttggagctgg tggaaaatcc aataactctt gaaattccgc catgaagtga
     2821 acttggctac cgcgctcgag tacgttcaca cccttcttgg tggcgatcac gggatcgccg
     2881 tcgaagtaag cggcgcgctg ctcgaactcg gtgaagtcct caaccgtctt caag
//
LOCUS       DASRKK010000489         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_626250, whole genome shotgun sequence.
ACCESSION   DASRKK010000489 DASRKK010000000
VERSION     DASRKK010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626250"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFC15_11385"
     CDS             <1..193
                     /locus_tag="VFC15_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10803.1"
                     /translation="SANVVMNRPAIEAGLSVLDAGGDFADGVIAYEGEWLGAEEFVSF
                     DSKAVSVLRSQGAHARLRS"
     gene            600..1067
                     /locus_tag="VFC15_11390"
     CDS             600..1067
                     /locus_tag="VFC15_11390"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZM10804.1"
                     /translation="MAENPHYKELLQLLNEFEVEYLIVGGFAVMKYSEPRYTKDLDVW
                     VHNSRQNSLRVVEALKKFGAPLDHDKVTAETFTDKQVVYQIGIAPVRIDILTEITGVR
                     FSAAWRQRVASTFFGIPVHFISFDDLVANKQALGRNSDLKDLKRNPGTSKSET"
     gene            complement(1380..1952)
                     /locus_tag="VFC15_11395"
     CDS             complement(1380..1952)
                     /locus_tag="VFC15_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10805.1"
                     /translation="MLTDRPPSTAAIRVRFLESDRLTALEVPEDDQLQALAKSIASAM
                     QGGTPASVRQACAEFLSVASGFYLVRQPGIRVLAARPLRVREGGSAIELFGDYHPETL
                     LIRLWMRTAIKKQVTSYGTFLSTLCHEFCHHLDCQRFGWRESWHTRGLYERTAALYHH
                     ARGTPVKPLFWVRAPGERWRIDWPRTTRGG"
BASE COUNT          474 a          573 c          605 g          424 t
ORIGIN      
        1 gagcgctaat gtcgtaatga accggccggc gatagaagcg ggcctatccg tgctcgatgc
       61 cggcggggac tttgcggatg gtgtaatcgc ttatgaaggg gagtggctgg gcgcagaaga
      121 gtttgtttcc ttcgactcga aagctgtctc tgtattgagg tcacagggcg cgcacgcccg
      181 cttgcggtca taacagccga gcgcggtgga atggcaatcg ctctgccctg tccctgatcc
      241 ttcctcacgc atgcaggttc cttctctgac gaagccaccc gacaggctac ccggcagcca
      301 aggctacagc gcagcgctga aaaagcggtc agagcggaag gcgcggaaga acccggcgtc
      361 tcctgcgcgt ccgaaggaag gaacagagtt ggccgttcga tcctgatatc ctgataatct
      421 taaagaccga ttctgcgaag gaaacatgag taatcctcaa aatttggcgt ggaaaacacg
      481 actggtggag ggcgggttcc ccgataggtc atttgatatc gaattctggc aggagcaggg
      541 ggatgaagcc atctttgcgg ccgcttggga actggtggaa ctagctgagg aagtcagaaa
      601 tggcagaaaa cccacactac aaagaactgc tacaactctt aaacgaattt gaggtcgagt
      661 acctgattgt aggcggattc gcagtcatga agtacagcga gccgcgttat acgaaggatc
      721 tcgacgtgtg ggtgcacaat tcaaggcaaa actctctccg cgttgtcgaa gccctcaaga
      781 agttcggtgc tccccttgac cacgataagg taaccgccga aacattcacc gacaagcaag
      841 tggtctatca gatcggaatt gctccggtgc gcatcgacat tctcaccgag atcacgggcg
      901 tcaggttttc cgcggcgtgg agacagaggg tcgcaagcac tttcttcggc atcccggtgc
      961 acttcatttc ctttgatgac ctggtagcca acaaacaagc attaggtcgc aacagtgacc
     1021 tcaaggactt gaagagaaat ccgggaacct cgaagtcgga gacgtagaca gagcccgcca
     1081 taggactggc ttgaccgcag cgtaggcttc gttggcgggg tccagccaat cgggactact
     1141 cgcggcaacc acaatccatt tctcgacgag ccggcggtga cccggatcga aaggttttcc
     1201 aactgccgcg ctcacccagg gaattcaccg cgccatggca agtttgcgtg acaaactgtt
     1261 ttcttttctt ccaacatcgc gaagatcttg accttcacct tataagcacg tccggtttgt
     1321 acccggctgc cgattaaccc aagctggccg catctggctg ccgacatttc gacgcaaagt
     1381 caccctccac gagtggttcg gggccagtcg atccgccaac gctcgccggg cgctcgcacc
     1441 cagaacaggg gtttcaccgg agtgccacgt gcgtggtggt agagcgcagc agtacgctcg
     1501 tataaaccgc gcgtatgcca cgactctcgc cacccgaagc gctggcagtc gaggtgatgg
     1561 caaaactcgt ggcagagggt gctcagaaac gtaccgtacg aggtgacttg tttcttgatg
     1621 gcggtccgca tccagagacg gatgagtagg gtttcgggat ggtagtcccc gaacaattca
     1681 atagccgaac cgccttcgcg gactcgaagc gggcgggctg caagtactcg gatcccgggc
     1741 tgccgaacca gatagaaacc agatgcaacc gacagaaact ccgcacatgc ctggcgaacg
     1801 gacgcgggcg tgccgccttg catggcggaa gcgatagact ttgccaacgc ttgcaactgg
     1861 tcgtcctcgg ggacttccaa cgctgtgagc cggtcgctct caagaaacct tacccgtatc
     1921 gctgctgtgc tcggaggtcg gtcggtaagc attgctggaa attgtcgact cagcgtgtcg
     1981 agatgccaat ctgggtttac agctgtttta tccagtcgcc tgtcttcatg acgcggctga
     2041 atcgcatctg ctgggtgacc aggatcgccc gatgca
//
LOCUS       DASRKK010000490         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_919823, whole genome shotgun sequence.
ACCESSION   DASRKK010000490 DASRKK010000000
VERSION     DASRKK010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919823"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFC15_11400"
     CDS             complement(<1..255)
                     /locus_tag="VFC15_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10806.1"
                     /translation="MPSLKTGHDAGRRRFWLAMSGFVMALALLPFSYKVERRLETAVH
                     IKGAESAKVDLELAERFQSPYAHPLVLVISGIPDPDSAEGA"
     gene            complement(728..1342)
                     /locus_tag="VFC15_11405"
     CDS             complement(728..1342)
                     /locus_tag="VFC15_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM10807.1"
                     /translation="MQTREGQGKNHQAYLWQYGRPGGIVVFDFRLGRGRDGPKRFLGQ
                     FQGILQTDGYAAYDQIGGPRMVHAACWSHARRQFFEAVQLNPRDPVATPIVARIDELF
                     SVDAEARRKALSVAARHVRRQETAKPLLDDIRSKIDAAQSVALPSSALGKACQSALTL
                     WRNLTRFLEYPELELSNNLAEDSMRPVALGRKNWIHIGSPQAGP"
     gene            1570..2205
                     /locus_tag="VFC15_11410"
     CDS             1570..2205
                     /locus_tag="VFC15_11410"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HZM10808.1"
                     /translation="MASSTLLTPSSGRSRFKTVLWISLGLTALFVFITSEALLVTDYS
                     MYHAYRLQVIADRHLLIPHTLCGVIALLAGPIQFSSRLRQRHLKFHRVLGRIYVVCVF
                     IGALTGIALAAGRPGLPGTSMQAAAWIVCTTAAFITARNRQIVQHRQWMVRSYAVTFT
                     FVSSRVLNLWPRYWSHLGDTFAAVGVIAFTLASLLIVDLGLNWRELTTRRA"
     gene            2562..>3185
                     /locus_tag="VFC15_11415"
     CDS             2562..>3185
                     /locus_tag="VFC15_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZM10809.1"
                     /translation="MKLQKFNIGIIGGGIGGVAAAVALHRAGIEVTVYERATEFREVG
                     AGMMLWPNATRVLKELGLLERVSASSGSSQHFLVRSSAGAVLMDIRLGQFDVPALCTR
                     RSDLLDALLSALPPERIRLDHDFESFEQRKTCVRVNFFGSVSGGGVSAEHDFVIGADG
                     IRSRVRSQLLGVHEPIYRGYTVWRGLARLMGAVPSGSNSETWGRGKRF"
BASE COUNT          648 a          983 c          865 g          689 t
ORIGIN      
        1 tgcgccttct gctgaatccg gatcgggaat accgctgatc accagaacca gggggtgggc
       61 gtacggagac tggaaacgct cggccagttc caggtcaact tttgcggatt ctgcgccctt
      121 gatgtgaaca gctgtctcca agcgacgttc gactttgtag gaaaagggca gcagcgcaag
      181 cgccatgaca aaccccgaca tcgccaacca aaatcggcgg cgaccggcat catggccagt
      241 cttcaagctt ggcattacca tcgaagatcc acgccgttta ctggttcgtc tgagagcaat
      301 gaaacgatct cggtgatcgg gcacatccat ggtacagggc caagctggat attcattggg
      361 ctgcggcctt gagcttagtt aacgaaagat gtaggaaact gcccgttaag cccttgtcaa
      421 tcgagggacc tagccgacga agacttcgct gtacgattac ccgccaatcg ctcatcaagg
      481 aacgaattag gaagctcaaa gaggattagt aatgataaac gatagatacc tgtcgggact
      541 gccccgacgc agaagtttac cccggtcgct tgcgtctatg aatgccgggc gacccacaca
      601 gcgggagtaa ggtctggaag gcacctgatc gggagatcgg caagccgggg agaaccctgg
      661 aaaactaatc gctcactggg acttgcagcc gacggcagct ttccacgacc gagagaatcg
      721 ctgcaaccta cggtcccgct tgtgggctgc cgatgtgaat ccagttcttc cgacccagag
      781 ccacgggacg catggaatcc tccgccaggt tattgctcag ctcaagttcc ggatattcca
      841 gaaatcgtgt aaggttcctc caaagcgtga gcgcggactg gcaagccttg ccgagtgcgc
      901 tggaaggcaa tgcaacggac tgcgctgcgt cgatcttgct acgaatatca tcgagaagag
      961 gcttggctgt ttcctgacgc cgaacatggc gcgcggcaac actcagggcc ttgcggcgag
     1021 cttcggcgtc gacggagaac agttcgtcta tccgcgccac gatcggagtt gccaccgggt
     1081 ctcggggatt cagttgcacc gcttcgaaaa actgtcttct tgcatgagac cagcaagcgg
     1141 catgcaccat tctcggcccg ccgatctggt cataagccgc gtatccatcg gtttggagaa
     1201 tgccttggaa ctgtcccagg aaccgcttcg gtccatcgcg tccacggcct aaacggaagt
     1261 cgaacaccac aatccctccg gggcgaccat attgccagag atacgcttgg tgattctttc
     1321 cctggccctc gcgtgtctgc acatcgaccg gggttcgtcc gcctgaatgt aacttccgct
     1381 ggtcaactct cgcttcatcg cggcgaccac aggaactagc aactcgccgc tctctttttc
     1441 atgcgatggc agcgatgctt gtaccgtcga gcctgatgaa cgccggctat gagacactat
     1501 caatatgaga tttctctcac cctgccctct tcgctccgtc attacgtcta tccatctaag
     1561 aggcgaataa tggcatcttc caccctgctc acgcccagtt ccggccgttc cagattcaaa
     1621 accgttctct ggatctccct tggtctcact gcgctctttg tgttcatcac ttcagaagct
     1681 cttctcgtca ccgattactc catgtaccac gcctaccgtc tgcaggtcat cgccgaccgc
     1741 catctcctca tcccccatac gctctgcggc gtcatcgctc tcctggccgg tcctattcag
     1801 ttctcgtcgc ggcttcgcca gcgtcacttg aaattccatc gcgtcctcgg ccgcatctac
     1861 gtcgtctgcg tctttatcgg cgccttgacc gggatagccc tcgccgccgg ccgccccggc
     1921 ctgccaggaa cctccatgca ggccgccgcc tggatcgtct gcaccaccgc cgccttcatc
     1981 accgcgcgca accgccaaat cgtccagcat cgccaatgga tggttcgctc ctatgcagtc
     2041 acgtttacct tcgtctccag ccgggtgctc aatctctggc cccgctactg gagccatctt
     2101 ggcgacacct tcgccgccgt cggcgtcatc gcgtttacgc tcgcttccct gcttatcgtc
     2161 gacctcggcc tcaactggcg cgagctcacc acccgtcgtg cctgatggtc gccgactatg
     2221 agagctatca atatgaaacc attcgcgccc ctcgcgcttg cggcactcct ccccgtgcaa
     2281 gacgaggttc tcatcctcgg cggtggagtg gcaggctgtg ccgctttgat cgcgctcgcc
     2341 gcaagggacg agagatattg actgtcgccg tggttcacat gaacaaatcg taatgaacga
     2401 aagtacatcg caggcgtcta aaataggtga atccctgtat cccaacgaac gaagaccgcg
     2461 attcctgaca aagcccgttg cggaaccggc aggtgcgtct ccgcgcagaa aagctacgcg
     2521 gctccgggct caacaagttt ggaaacgtaa acctgaatca aatgaaattg cagaaattta
     2581 atatcggcat catcggtggc ggaattggcg gcgttgccgc ggcggtggcg ctccaccgcg
     2641 cgggcattga agtgacagtc tacgagaggg ctaccgagtt ccgcgaagtc ggcgcgggta
     2701 tgatgctctg gcctaacgcc acgcgcgttc taaaggaatt gggcctgctt gaaagggtat
     2761 cggcatcgag cggttcaagc cagcattttc tcgttcgctc cagtgcgggt gcggtcctga
     2821 tggatattcg gctcggccag tttgacgtgc ccgcgctatg cacacgccgc tcggacctgc
     2881 tggatgcgtt gctctcggca ctgccgcccg aacggatccg cctcgaccac gatttcgaat
     2941 ccttcgagca gcgaaaaacc tgcgtcagag ttaacttctt cgggagtgtg tcaggaggag
     3001 gtgtctctgc ggaacacgac ttcgttatcg gtgccgatgg tatacgctcg cgcgtccgtt
     3061 ctcagctatt gggagtccac gaacccatct accggggcta caccgtctgg cgcgggctgg
     3121 cgcgtcttat gggcgctgtt ccgtccggct cgaacagcga aacctgggga cgaggaaaac
     3181 gcttc
//
LOCUS       DASRKK010000491         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_431261, whole genome shotgun sequence.
ACCESSION   DASRKK010000491 DASRKK010000000
VERSION     DASRKK010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431261"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFC15_11420"
     CDS             <1..1041
                     /locus_tag="VFC15_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZM10810.1"
                     /translation="DRVATMYNKIGAVHGGDRVEVLEKQRRFLRVRTDSGQEGWIEER
                     SLVPQAVYDAFQKLASDNASQQVQAHGTARSELNMHVTPAREGEHLYQLKDGEKVEVL
                     KRATAEKNPPKSASANATQSPKSAATTPPQSKTAPVPAKPPPSAKAGSAPGKPPAPPK
                     PVMEDWYLVRNSAGRVGWVLLRMIDLDIPLEVAQYAEGQRIMGYFVLNTVQEEDKQVP
                     QYLVLLNEPKDGLPWDFNQIRIFTRNRAKHRYETAYRERNLEGYFPVKTGHQDFGKEG
                     DLPTFTIRKKTDDGQMVDITYKLNGPIVRRVLTPEEEAAEKTKHEAALAAYYAQRKKQ
                     PPAHPQPHHKHK"
     gene            complement(1251..2396)
                     /locus_tag="VFC15_11425"
     CDS             complement(1251..2396)
                     /locus_tag="VFC15_11425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZM10811.1"
                     /translation="MLDLTRQYETIKDDVHAAIERVLTTQHFIGGPELEGFERESANY
                     LGVSASVGCASGTDALWLALAAAGVGPDACVITTPFSFFASVSAIVRCGATPILADID
                     PATLNLDPEATERALKRGRSYQVRAVMPVHLYGQCANMDKFGRLAEQYDASLVEDAAQ
                     AFGAKWRDKKAGALGKAAAFSFYPTKNLSAYGDGGCVTTNDEELAAHVRRLRNHGSRQ
                     RYYHEEIGWNSRLDAMQAAVLRVKLKHIDEWNQARRMLACRYHGLFINSGLVQGGAQP
                     VSAPAPIALLATMPEAYHIYHQYVVRAFRRDELRSFLTEQDIGTEIYYPVPLHLQPCF
                     AYLGYKAGDLPESERASKEVLALPMFPELREDEQQRVVAAIAEFYSR"
     gene            2911..4524
                     /locus_tag="VFC15_11430"
     CDS             2911..4524
                     /locus_tag="VFC15_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM10812.1"
                     /translation="MISAKLKAGALTAVLGLTLSAPLLAQSQSVAPTTTINHVLLISV
                     DGLHALDVARYIQDHPNSALAQLSQHGVTYSNARTPANSDSFPGLLALVTGGSPVSHG
                     LFYDVSYDRTLFDPTNTTCSGGAGNMMVFDESIDLYGLNHRSLNVIDPTKLPHRLDSG
                     GHCVRVYPHDAMRTNTLFEVVKAAGGRTAWADKHPAYDLVNGPSGTGVDDLYTPEITN
                     VGGLDNTHSVVCTVKNDYLKVAAIINEINGLNHDGTRHPGTPVLFGMNFQAVSVGQKL
                     AKDNYDHSCTDDTDPQINQQSGGYVDGAGTPTAVLDYGLRKTDEALASMITALQNRGL
                     YDSTLFIVSAKHGQSPINPLKINKPGHFADLVAALPDGHSNPAAIAITNAANCSTGPC
                     GFVQDDDIALIWLQGQPVTDQQVADYLNTNANALFIDEVMGGAELTLKFNDPGHDSRT
                     PDVIVQPLYGTVYTGSTAKNAEHGGFSFGDTNVGLIVSLPSFPASVVKTPVATSQVAP
                     SILHELGIDPQSLHSVQTEKTELLPGLPQ"
     gene            5119..5715
                     /locus_tag="VFC15_11435"
                     /pseudo
     CDS             5119..5715
                     /locus_tag="VFC15_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
BASE COUNT         1292 a         1734 c         1722 g         1117 t
ORIGIN      
        1 gatcgagtgg ccactatgta caacaagatt ggtgcggtgc atggcggcga tcgcgtggaa
       61 gttctggaga agcaaaggcg tttcctgcgg gtgcgtaccg acagcggaca ggaaggctgg
      121 atcgaggaac gcagcctggt cccccaggct gtttacgatg cctttcagaa gcttgccagc
      181 gacaatgcca gccagcaagt gcaagcccat ggaacggccc gctccgaact caacatgcac
      241 gtaacgccgg cgcgcgaggg cgagcacctt tatcaactca aggacggaga gaaagttgag
      301 gtcctgaagc gcgccacggc ggagaagaat ccgccgaagt ctgcgtccgc gaatgcgacg
      361 cagagtccga agagtgccgc gacgactcca ccgcaatcca agaccgcgcc ggtgccggct
      421 aagccgccac cttccgccaa agcaggcagc gccccgggca agccgccagc gcccccgaag
      481 cccgtgatgg aagactggta tctggtgcgt aactccgccg gacgcgtcgg ctgggtgcta
      541 ctacgcatga tcgacctcga tatcccgttg gaggttgcgc aatacgccga gggccagcgc
      601 atcatgggct acttcgtgtt gaacaccgtg caggaggaag acaaacaggt gccgcagtac
      661 ctggtgctgt tgaacgagcc caaggacgga ctgccttggg actttaatca gattcgaatc
      721 tttaccagga accgggcgaa gcatcgctat gaaaccgctt atcgcgagcg caatctggaa
      781 ggctacttcc cggtaaagac tggtcatcag gatttcggga aagaagggga cctgccgacc
      841 ttcaccatcc gtaagaaaac tgacgatggg cagatggtag acatcactta caaactgaat
      901 ggccccatcg tacgccgagt gctgaccccg gaagaagagg ctgcggagaa gaccaaacat
      961 gaagccgcgc tggccgccta ctacgcacag cggaagaagc agccgcccgc gcatccgcag
     1021 ccgcaccaca agcacaagtg agagttgcta ttgaggattg cgaattatag tgacacgtcg
     1081 gcaataaaca tgagtgggtc atcacttaag aattgtcatt acgagcgccc tggttttggg
     1141 cgcgaggaat ctgctgttcg tggaaaaggc aaaaagcagg tccctcgcta cgctcgtgat
     1201 gacaaacgtt acaaaatgtc actcgattgt gcgagcctca ataggtcgag ctatcggctg
     1261 tagaactcgg cgatggcggc gaccacgcgt tgttgttcgt cctcgcgcag ttcggggaac
     1321 ataggcaggg ccaatacttc tttcgacgcg cgctccgact ccgggagatc gcccgcttta
     1381 tatcccaggt aagcgaagca tggctgcaag tgcagcggga cgggatagta gatttccgtt
     1441 ccaatgtctt gctcggttaa gaaacttcgc aactcatccc ggcgaaaggc gcgcaccacg
     1501 tactggtggt aaatgtgata cgcctcgggc atcgtcgcca gcagcgcgat aggcgccggc
     1561 gcgctgaccg gctgcgcgcc gccttgcacc aggccggagt tgatgaacaa accgtgatag
     1621 cggcaagcca gcatccggcg cgcctggttc cactcgtcaa tgtgcttcag cttcacccga
     1681 agcacagcag cctgcatggc atcgaggcgg ctgttccagc cgatctcttc gtggtaatac
     1741 cgttggcggc ttccatgatt gcgcaaacgt cgaacgtgcg ccgccagttc ctcgtcattg
     1801 gtggtgacgc atccgccatc gccataggcg ctcaggttct tggtaggata aaaactgaac
     1861 gctgcggcct tgcccagggc tcctgctttc ttgtcgcgcc actttgcacc gaaagcctgc
     1921 gcagcgtctt cgacaaggct ggcatcgtac tgctcggcga ggcggccgaa tttgtccatg
     1981 ttggcgcact gcccgtaaag gtgtacgggc atcaccgcgc gaacctggta cgaacgtccc
     2041 cgcttcagcg cccgctcagt ggcttcagga tcgagattca gtgttgctgg atcgatatcc
     2101 gccagaatgg gtgtggcccc gcagcgcaca atcgcactta ccgaagcgaa gaagctgaag
     2161 ggcgtggtga tcacgcacgc gtccggtcca acaccagcgg ctgccagtgc cagccacaag
     2221 gcgtcagttc cggacgcaca gccaacactc gcgcttaccc ccagatagtt tgccgactcg
     2281 cgctcgaagc cctcgagttc cgggcctccg atgaagtgtt gggtagtcag cacccgttcg
     2341 atggccgcgt gaacgtcgtc ctttatggtc tcgtactgac gagtaaggtc aagcataggg
     2401 acaggatcgc gccgggtcgc agtacctgga ctgacaaaag attgtggcga ggtcaatggc
     2461 atgacggaac cttgattcta tagcggaatc gacgcttggt gtacgcggac tggcgcggcg
     2521 ggtggtcgct cggcttccgg attggcatct actcgccagc aaccggcgtt ttccggagat
     2581 tgtgagtgta atggcgatta cttccgagta gccgaccatc cagccagagt tgttggattt
     2641 cttgcggacg acgaacggca ggtcccttgc tccggggcat cggtaaattc gggatgccaa
     2701 tagaacccat agttactact gtgaaaagcg tatcgcgtcc acactagcac ctttctgcgg
     2761 acctagcact tgagtcagcg cgtgtcttca gcgcaggtgt gctgagtaaa ccatggtcgt
     2821 cttttcatcg actgttcaca tccgcgtaac ccggatgcaa actgctctcc ttaaggttga
     2881 cgctccaggt tcaactctag aggaggagtc atgatttcag caaagctgaa ggctggcgca
     2941 ttgacggcgg tgctcggtct cacgctgtcc gccccgcttc ttgcccagtc acagtccgtc
     3001 gctccgacca ccaccatcaa ccacgtgctt ctgatcagcg tcgatggcct gcacgccctc
     3061 gacgtggcgc gctacatcca ggatcaccct aattctgctc tggcccaact gtcgcagcac
     3121 ggtgtcacct attccaacgc ccgtacgccc gccaactccg attcgttccc cggtcttctg
     3181 gccctggtaa ccggcggttc gccggtctct cacgggttgt tttacgacgt cagctacgac
     3241 cgcaccttgt tcgatcccac caacaccacc tgctccggcg gggcgggcaa catgatggtc
     3301 tttgacgaga gcatcgacct ctacggcctc aaccaccgat ctctcaacgt cattgacccg
     3361 accaagctgc cccaccgcct ggacagcggc ggacactgcg tgcgggtata tcctcacgac
     3421 gccatgcgga ccaacaccct ctttgaagta gtgaaggctg ccggcggccg cacggcatgg
     3481 gccgataagc atcccgccta cgatctggtg aatggtcctt ccggcactgg cgtggatgat
     3541 ttgtacacac cggaaattac caatgtagga ggattagata acacccacag cgtggtttgc
     3601 acagtcaaaa acgattacct gaaggtcgca gcgatcatca acgagatcaa tggtctgaac
     3661 catgacggaa caaggcatcc cgggacccct gttctgtttg gcatgaactt ccaggcggtg
     3721 agcgtggggc agaagctggc caaggacaac tacgaccata gctgcactga cgataccgac
     3781 ccacaaatca accagcaatc cggtggttac gtggatggcg ctggaacccc gactgctgtt
     3841 ctcgattacg gcctacgcaa gaccgacgag gcgctcgcca gcatgattac ggcgctccag
     3901 aacaggggcc tgtacgactc gaccctgttt atcgtcagcg cgaagcacgg ccaatctccg
     3961 attaacccgc taaagatcaa caagccgggc cacttcgcag acctggtcgc agccctgccg
     4021 gacggtcaca gcaacccagc cgcgattgcc atcacgaacg ccgcgaattg ctccactggc
     4081 ccgtgcggtt tcgtgcaaga cgacgatatt gcgctgatct ggctccaggg ccagcccgtc
     4141 accgaccagc aggttgcaga ttaccttaac acgaatgcca atgcattgtt catcgatgag
     4201 gtgatgggcg gagccgaact cacgctcaaa ttcaatgatc cgggacacga cagccgcacc
     4261 cccgacgtta tcgtgcagcc cttgtacggt accgtttaca ctgggtcgac cgcgaagaat
     4321 gccgagcacg gcggtttcag cttcggcgac accaacgttg gcctgattgt ttccctgcct
     4381 agcttccctg ccagcgtggt gaagacgccg gtggctacct cgcaagtggc gcccagcatc
     4441 ttgcatgaac tcggcatcga tccgcagagc ctgcactcgg tgcagaccga gaaaacggag
     4501 ctgctgcccg gcctgcctca ataacccgct tactactcct gcgaaaacca cggacgggcc
     4561 gctgaaggcg gcccgtccgt gtaatgtcac agcgaccaat gttcagcgcc cgcgttcgat
     4621 tcacaagcac gccgccgggt ggggaagtga aattctcggc gagttacggg ctactcggag
     4681 tgcgccggga taaaccggca tgttgtaggg gatgcaagag ccctacagga aaggaagtag
     4741 cgaatccatc ctggcctcga gtcttgcagg ggacatcgtg agatatttct tgaagcgtag
     4801 accagaggca tcggtgggcg gggctattga gctgcgaaaa gcccgtagat caggatgccg
     4861 acttcattcc gagtaggagg aaggcaacac ggcagccacg ccaatcgcga gtggactgtc
     4921 ggtcctgcgt agtctaagaa cccgcgcacg ctgagaaaca acatgcacga gaaccgggag
     4981 atctccagca cgccttggtc ccatggtcaa ggccggtccg cgaaggccga aagccgcacg
     5041 gcggacatgc acgtgctgga gaagtcggac tgtgccgtag tacctgtgaa ccagccgaac
     5101 caggaagggc aaccttccgc ggaggctggg gagggaaggg cgcagacgag ggagaacatc
     5161 gttcaatcgc acatgcaccc gacgcagagc gggaagcgca tgtcccaggg gttggacggt
     5221 gtgcggaagg cagcaaggga aagaaagcag gagcggttca ccgctttgct ccaccatctc
     5281 aacgttgatc tactccgcga cagcttctac gcgcttcagc gtaaggcttc gcccggagta
     5341 gacggcgtaa cgtggcagga atatgaaacc gggctcgagg atcggcttgt cgatctgcac
     5401 agccgggtgc accgtggaac gtatcgggca aagccttcaa gacgagtctt cataccgaag
     5461 gctgatgggc ggcaacgtcc gttgggcatt gcagcgctgg aggacaaaat cgttcaacag
     5521 gcggtggtga cgatcctcaa ccagatatat gaggtcgact tcaaaggctt ctcttacggg
     5581 ttccggcccg gtcgcagccc gcatcaggcg cttgacgcgc tgacggtggg catccaacgg
     5641 aagcgagtga actgggtgct tgatgccgac atccgtggct tcttggattc atcctcacac
     5701 tgctcctatg gttgatcgaa gaggccggtt ggaggatcta cgactttgat tcgcaagcct
     5761 ttgcgctgtc cctgccgaac atggagagct tcgagttcgc cgcgctttac acgctgccac
     5821 aaagtttgcc gagagatgcc gagtcgccgc atggcgtcca cgacg
//
LOCUS       DASRKK010000492         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_529461, whole genome shotgun sequence.
ACCESSION   DASRKK010000492 DASRKK010000000
VERSION     DASRKK010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529461"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VFC15_11440"
     CDS             <1..1515
                     /locus_tag="VFC15_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10813.1"
                     /translation="IFSGSHFPGSISYAKAYNSEGQFAVPGLPNFTTHGNSDTFGINW
                     SEFVPGLPTLTASFQTGSNQYSVYGANSNGNSNFHSFSLNSNYSIAGFNLGASYQTGV
                     SNSVIPQVFQGEQTGTVTTDNSGFGVNVSHRLPMQGEFTTNFYRSNIDTDYFGYSYKG
                     TFDTAVAAAAVQPTDKLHVSVTASYTDNLAGILFQSLISSGGSLLPTNNLEASHAWDL
                     NGAATYSVAPNLQLQAQADRRDQTYLGESFGATSFSAGLSYAHGFLGGALNSSLSVID
                     SIIDNSSENALGFSTNANYTRRIGRWFVGGSFNYAQNVSTLLVTYTTSYYSYSGNVRR
                     RFGNFNWSAAANFSHSGLTNLPGTNSSSQSYTTSFGYGRWINANANYAKSNGIGLITG
                     GGVLPVNLPPLIPSDLITLYGGTSYGVGLGSSPLRRLTVSAAYSKASTNTLSAGIGSS
                     NQSEGANALLQYQFRKMYLTGGYAHISQGFSVGDLAPAKVSTYFIGVTRWFNFF"
     gene            1497..>2459
                     /locus_tag="VFC15_11445"
     CDS             1497..>2459
                     /locus_tag="VFC15_11445"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZM10814.1"
                     /translation="MVQLLLAGARTRVPHFFSNVACVVLLLLTPVWASKPKEKPNQPA
                     PPDMVLEGGRKLSYERSFNSQREVKPKRGFWNRVLDFVAGEPEFRSLIRPYSIAVDSR
                     GRIIVTDPGAIGVHIFDFQQQKYKFLSRAGKGKDPMLAPQCVAVDAHDNIYVTDSDTG
                     KVFVFDSEGKYQRAIGSLKGGEGFFKRPTGIAVDSSAQRIYVTDTLRNKIFVMDLQGN
                     VVQTIGENGSGPGKFNFPTELRLDGPNLIVVDAMNFSVQVLDRSGAYKYAVGKIGDSP
                     GTMFRPKGIGLDSEGDLYVVDGLWGVVQVFNREGDLLYYFGNRGT"
BASE COUNT          525 a          710 c          696 g          528 t
ORIGIN      
        1 atcttcagcg gcagccattt tccgggatcg atcagctacg ccaaggcgta taacagcgag
       61 ggacaatttg cggttcccgg gctgcccaac ttcaccaccc acggcaatag cgacacgttt
      121 gggattaact ggagcgagtt cgttcccggc ctgcccaccc tcacggcaag tttccagacg
      181 ggcagcaacc agtactcggt gtatggggcc aatagcaatg gcaactccaa ctttcattcc
      241 ttcagcctca actcaaatta ctcgattgcg ggtttcaacc taggggcctc ttaccagact
      301 ggagtttcca actccgtcat cccgcaggtt ttccaaggcg agcaaacggg gacagtcact
      361 accgacaaca gcggtttcgg ggtgaatgtg tcgcatcgcc ttcccatgca gggagagttc
      421 accaccaact tctaccggtc aaatattgat acagattatt ttggctatag ctacaaggga
      481 accttcgaca ccgcggtcgc cgctgcggcg gtgcaaccta ccgataagct gcatgtgtca
      541 gtgacggcca gctacaccga taatcttgct ggaattctct ttcaatcgct aatctcgtcg
      601 ggcggtagcc ttctgccgac aaacaacctg gaggcctccc atgcctggga cctaaatggg
      661 gcggcaactt attccgtggc gccgaacctg caactccagg cgcaagccga tcgccgcgat
      721 cagacttacc tcggagaaag cttcggggca acttccttta gcgcgggcct cagctacgcc
      781 catggttttc tcggcggcgc actgaacagt tcgctcagcg tgattgatag catcattgac
      841 aacagcagcg agaatgcgct cggtttctcg accaacgcca actatacgcg caggatcggc
      901 cggtggttcg tgggcgggtc tttcaactat gcgcagaatg tctcgaccct gctggtgacc
      961 tacacgactt cgtactacag ctattcgggg aatgtgagac gccgcttcgg caatttcaat
     1021 tggagcgcgg ccgcgaactt tagccacagt ggcctgacta acctgcccgg cactaacagc
     1081 agcagccagt cttacacgac cagctttggg tacggacggt ggatcaatgc caatgccaat
     1141 tacgccaagt cgaatgggat cggactcatt accgggggcg gagtgctccc cgtgaacctg
     1201 ccgccgctga taccatcgga cctgatcacg ctttatggcg gaaccagcta cggagttggg
     1261 cttggaagtt cgcccctgcg caggctaacc gtgtcggcag cttactccaa ggcctcgacc
     1321 aatacgttga gtgcgggcat cggttcttcc aaccaatctg agggagcgaa tgcgctgttg
     1381 caataccaat tccgtaaaat gtacttaacg gggggatacg cgcacatttc gcagggcttc
     1441 agcgtggggg accttgcgcc ggccaaagtg tccacgtact tcattggagt gactaggtgg
     1501 ttcaacttct tttagcggga gcgcgcacga gagtcccaca tttcttctca aacgttgcct
     1561 gtgttgttct tcttctgctg actccggttt gggcgtccaa gcccaaagag aagcccaacc
     1621 agcctgcgcc tccggatatg gtgctggaag gcggtcgcaa gctcagctac gaacgcagct
     1681 tcaactccca gcgcgaagtg aagccgaagc gcggtttctg gaaccgggtg cttgacttcg
     1741 ttgccggcga accggaattc cgtagtctta tacgtcccta tagcatcgca gtcgattccc
     1801 gcgggcgcat cattgtgacc gacccgggag cgatcggggt ccacattttc gatttccagc
     1861 aacagaagta caagttcctc tcgcgcgccg gcaaagggaa ggatcccatg ctggcgccgc
     1921 agtgcgtcgc cgtggatgcc cacgacaaca tctacgtcac cgattcggat acaggtaagg
     1981 tgttcgtctt cgactccgag ggaaagtacc agcgggcgat tggcagcctg aaaggcggcg
     2041 aggggttttt caagcggcct accggcattg ccgtcgattc ctctgcccag cggatttacg
     2101 taaccgacac gctgcgcaac aaaatatttg ttatggactt gcagggcaac gtggtgcaga
     2161 cgatcgggga gaacggtagc ggcccgggca agtttaattt tcccaccgag cttcgactgg
     2221 acggccccaa cctgatcgtg gtggacgcca tgaacttcag cgtgcaggtg ctggaccggt
     2281 ccggagccta taagtacgcg gtaggcaaga tcggcgacag ccccgggacc atgttccgtc
     2341 ctaaggggat tgggctcgat tccgagggcg atctctacgt ggtggacggg ttgtggggcg
     2401 tagtgcaggt gttcaaccgt gaaggcgatc tgctgtacta cttcggcaat cgcggcacc
//
LOCUS       DASRKK010000493         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1213610, whole genome shotgun sequence.
ACCESSION   DASRKK010000493 DASRKK010000000
VERSION     DASRKK010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213610"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFC15_11450"
     CDS             complement(<1..1322)
                     /locus_tag="VFC15_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM10815.1"
                     /translation="MRRKILWLVVFLMPGSFYVAAQTTSPTAGMPAPAQPAASASAPS
                     QMQTIPGTQTPAQLPTSGALLRLTVQGAEALALKNNPQISVFRLLALASKQVTREAKS
                     AYYPNLYGSLTAVKPEEGSRIAAGSLNNPIVYERAAGGLTLSQLITDFGRTNNLVATS
                     ALRAKAADMNALATSAQIKLAVDQAFYNALQTVALLKVAEQTVAARQLVSDQITTLFQ
                     NKLRSQLDVSFANANLAQGRLLLLDAQNNQQAALSSLSEILGYSSQQPFDLVDSESEL
                     KPPPDSVSQLVDSAFSNRPEIAAQNYEYQAAQHFQKAERDLLFPSFEALGVVGRTPYS
                     NTIAGVAPFTSWYGAVGVNVNIPIFNGFLYPARAREAGLRAQADEEQLRDLKDRIAND
                     VRASWLNALTAYKRISVTQQFVDQTNLAVDLSQTRYNLGLSSIVELSQA"
     gene            complement(1319..4465)
                     /locus_tag="VFC15_11455"
     CDS             complement(1319..4465)
                     /locus_tag="VFC15_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM10816.1"
                     /translation="MPSFAIKYPFFVLMLCLAIVVVGVTTVARMPVDLFPPIDIPVVV
                     VATFYGGMPPQQVEANITDTFERFFTLGSNIDHIESRSLTGVSLIKVYFEPGTDADAA
                     ASEIANLAMADLRRLPQGTLPPVVLKFDASSLPVCLVTLKGKGLNETDLHDQGQFNVR
                     NQIANVPGASVPQPYGGKYRQIQFYVDPVKLEAAQMSPMDVVNVINDSNKILPAGDVR
                     IGPKDYNIYSNSQLPDVSQMNDVPLKAIGNGVLRFGDIGHAEDSSAIQYNIVRVDGQK
                     SVYLPILKQGGNSNTIQIVDGVRDRVKHLLDIPSSLVANVVFDQSQYVKIAVANVLRE
                     GGIGLVLTAVMILIFLGSVRGTISVLISIPVSCLAAFLLLNGFGGTINTMVLGGLALV
                     LSRLIDNSVVVLENIFRHMEEGEDAVTAAERGGQEVQLAVLAATFTTAIVFFPVIFLY
                     GVSKYLFTALALGVVLALAASYLVAMTVVPLFCAKFIKTAHGHGGHQETRSIFGKFVA
                     GFNHRYNRMLGRYDAAVAKALNRPVATVVLIMGSFVVSLALAPLLGVAFFPRTDPGQF
                     VINVKAPTGSRLEMTNQYIARVEDDIRGVIGKDDLQMIVSNIGMTPDLSAIYTSNSAQ
                     HTAFVQVSLNKEHKVSTFTYMDRVRRKLDSDLPELSTYFQTGGLVDSVVNMGMPAPID
                     IQVSGNNQHAAYAIASEMAAKVRQLKDVSDVLIPQDLDYPGIELNVRREMAARLGLTA
                     TDVVDNVVTALTSNGMIAPSYWVDPHSGNNYFLTVQYNTHQLDSMTMEDFKQIPLHAK
                     DNSSTTMLENVADIKMINTPTEVDHYQLFRKIDVYVSPKGEDLGSLAGRVQKIIDDTK
                     LPANIRVEMRGSVKAMHQSFLSFGVGLLLAIVLVYLILMAQFASFLDPFIILLAIPSG
                     ITGVILFLLLTGTTLNVMSLMGVLMMTGIVVSNSILIVDVTRKYREAGMPIREAVALA
                     CRVRLRPILMTSLATLLGLVPMALALEAGSEQYAPLARSIIGGLLVSVVLTVFIVPAA
                     YLLIHRREEAQPPQEVQA"
     gene            complement(4472..>5273)
                     /locus_tag="VFC15_11460"
     CDS             complement(4472..>5273)
                     /locus_tag="VFC15_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM10817.1"
                     /translation="LKQASTARPGLIAEQELDDAEAKDRATEAQVEAAKSALAGARQQ
                     LEVSQANNSQVSAMSDYKRITAPFDGVITWRYADTGALVQAGTSNSNSAPVVKLAQVN
                     ILRLRIPVPESIAAKVQIGDAADVKVQAMGEHFTGKVARFTGALDMSTRTMQVEIDVP
                     NPGYHLQPGMYADVVLAADGRKNVLIVPVNAIQRDQNKTSVLVVDPQNRVQAREVQTG
                     MQSSNNVEIVAGLSAGERVIVGNLGSYQPGELVRPKEAVFAANSADSE"
BASE COUNT         1081 a         1554 c         1589 g         1049 t
ORIGIN      
        1 gcctgactca attcgacaat cgaactcaag ccgaggttgt agcgggtctg cgacaaatct
       61 accgccaagt tggtttggtc cacgaactgc tgcgtaacgc tgatgcgctt gtaagcggtc
      121 agcgcgttga gccaactggc acgaacgtca ttggcaatcc ggtctttcaa atcgcggagt
      181 tgctcttcgt cggcctgtgc gcgcaaccct gcttcccgcg ctcgcgcagg ataaagaaag
      241 ccgttgaaga ttggaatgtt gacattgact cccacagctc cataccaact cgtgaatggc
      301 gcgacgcctg cgatggtatt gctgtatgga gtacggccca cgacacccag ggcttcaaaa
      361 ctgggaaaca gcaggtcccg ctcggccttt tgaaagtgtt gcgcggcctg gtactcgtag
      421 ttctgtgcag caatttccgg acgattgctg aaggccgagt ccaccagttg gctgacggaa
      481 tcgggcggag gcttcaactc cgattcggag tccaccaaat cgaacggctg ttgcgaagaa
      541 tatcccagga tctccgagag cgacgaaagc gcggcctgct ggttattctg cgcgtcgagc
      601 agcaacagcc tcccttgcgc cagattggca ttcgcaaaac tgacgtcgag ttgcgatcgt
      661 agtttgttct ggaacagggt cgtgatctgg tccgacacca gttgccgcgc cgccacggtt
      721 tgctcggcaa ccttcagcag agcgaccgtc tgtagtgcat tataaaaagc ctggtccacc
      781 gcaagcttga tctgcgcact ggtcgccagc gcattcatat cggccgcttt ggcgcgaagc
      841 gcggaggtag cgaccagatt gttagtacgc ccaaaatcgg taatcagctg cgacaaggtg
      901 agcccacctg ctgcacgctc atagacaatc ggattgttga gactgcccgc tgcgatacga
      961 cttccctctt caggcttgac cgccgtaagg cttccataca ggttcgggta gtaagcggac
     1021 ttcgcttccc gcgtgacctg cttcgaggcc agcgccagca agcggaaaac agaaatctgc
     1081 ggattgttct tcagcgctag tgcctccgcg ccttgcacag tcaggcgcag cagcgctcct
     1141 gacgtcggca actgcgccgg tgtctgagtg cccgggatgg tctgcatttg cgacggcgca
     1201 gacgcggaag ctgctggttg tgcgggcgcc ggcattccag cggtgggcga agtggtttgc
     1261 gccgcgacat agaagctgcc aggcatcagg aatacaacaa gccagaggat cttacgtctc
     1321 aagcctgcac ctcctgcggt ggctgcgcct cttccctgcg gtggatcagc agatacgccg
     1381 caggaacaat gaacacggtc aaaaccaccg agaccaggag gccgccgatg atggagcgcg
     1441 ccagcggtgc atactgctcg ctgccggctt ccagtgccag ggccatgggg acaagcccca
     1501 acagggtcgc cagcgaggtc atcagaatgg gccgcaggcg aacccgacag gcgagggcaa
     1561 cggcctcacg gatgggcatg cctgcttcgc gatacttgcg agttacgtcc acgatcagaa
     1621 tgctgttcga tacgacgata ccggtcatca tgagcacgcc catcagcgac atgacgttca
     1681 gcgtggtgcc agttaggaga aggaaaagaa tcaccccggt gatgcccgac ggtatggcca
     1741 gcaggatgat gaagggatcg aggaaggacg caaactgcgc catcaggatc aggtaaacca
     1801 gcacgatcgc cagcagcagt ccgacgccga aactcaagaa cgactggtgc atggccttta
     1861 ccgagccgcg catctcgact cgtatgttgg ccgggagctt cgtgtcgtca ataatcttct
     1921 gcacccggcc agcgagcgag cccaggtctt cgcccttggg cgagacatac acgtcgatct
     1981 tgcggaagag ctggtagtgg tccacctccg tcggcgtgtt gatcatcttg atgtcggcca
     2041 cgttctccag catggtggtg ctgctgttgt ccttggcgtg caacgggatc tgcttgaaat
     2101 cctccatggt catcgagtcg agctgatgag tgttgtactg caccgtgagg aagtagttgt
     2161 tcccgctgtg tggatcgacc cagtaactgg gagcaatcat gccgttcgag gtcagggcgg
     2221 tcaccacgtt atcgacaacg tcggtagcgg taagcccgag gcgggccgcc atttcccggc
     2281 gcacattcag ctcgatgccg ggataatcca ggtcctgcgg gattagcacg tcgctcacgt
     2341 ccttcaactg ccgcaccttt gccgccatct cgctggcgat ggcgtacgcc gcgtgctggt
     2401 tgttgccgct cacctggatg tcgatgggag cgggcatgcc catgttgacc accgaatcca
     2461 ccaggcctcc ggtttgaaag taggtggaga gttcgggcaa gtcggaatcg agcttccgtc
     2521 gcacacgatc catgtaagtg aaggtgctga ccttgtgctc cttattcaag ctcacctgga
     2581 cgaaggccgt gtgctgggcg gagttgctgg tgtagatggc ggacaagtcc ggggtcattc
     2641 cgatgttgga gacaatcatc tgcaggtcat ctttgccgat gaccccgcgg atgtcgtcct
     2701 ccacccgggc gatgtactgg ttggtcatct ccaggcggga gccggtgggc gctttcacgt
     2761 tgatcacaaa ctggccgggg tcggtacgcg ggaaaaaggc gacaccgagc agcggggcca
     2821 gcgccaggct caccacaaag ctccccatga tgagaaccac ggtagcaacc ggccgattca
     2881 gcgctttggc aaccgcggcg tcatatctgc ccagcatccg gttgtagcga tgattgaagc
     2941 ccgcaacgaa ttttccgaat attgacctgg tttcctggtg cccgccgtgc ccgtgggccg
     3001 tcttgatgaa cttcgcacag aacaaaggga cgacggtcat cgccaccagg taagaggcgg
     3061 cgagggccag taccacgccg agcgccaagg cggtgaacag gtacttgctg acgccgtaca
     3121 ggaaaatgac ggggaagaaa acaatcgcgg tggtgaacgt ggcagccaac accgcaagct
     3181 ggacctcctg gcctccgcgt tcggcggcgg tgaccgcatc ctcgccctcc tccatgtggc
     3241 ggaagatgtt ttccagcacc accaccgagt tgtcgatcag tcgcgagagc accaaagcga
     3301 ggccgcccag caccatggta ttgatggtcc cgccgaagcc gttcaacagc aggaacgcgg
     3361 ccagacagga gaccggaatc gagatcaaaa ccgagatggt cccgcgcacc gagcccagga
     3421 aaatcaggat catcaccgcg gtcagtacca ggccgatgcc gccctcgcgc agcacattgg
     3481 ccaccgcgat cttcacgtat tgcgactggt cgaagaccac gttcgccacc aacgaggacg
     3541 gaatatccag cagatgcttc acccgatcgc gtactccatc cacaatctgg atggtgttgc
     3601 tgttcccgcc ttgtttcagg atgggcagat agaccgactt ctggccgtcc acgcgaacga
     3661 tgttgtactg aatggccgac gagtcttcag cgtgcccgat gtctccgaag cgaagcacgc
     3721 cgttgccaat ggccttcagc ggcacgtcgt tcatctgcga gacatcgggc aactggctgt
     3781 tgctgtaaat gttgtagtct ttggggccga tgcgaacgtc gcccgcgggc aggatcttat
     3841 tggagtcgtt gatgacattg actacgtcca tggggctcat ttgcgccgcc tccagcttca
     3901 cgggatcgac gtaaaactgg atctggcgat acttgccgcc atagggctgt ggtaccgatg
     3961 cgcccggcac gttggcaatc tggttgcgaa cgttgaactg tccctggtcg tgcagatcgg
     4021 tctcgttgag ccccttcccc ttcagggtca caagacaaac cggcaggctg gaggcgtcga
     4081 atttcagcac cacaggaggc agcgttccct gcggcaaacg ccgcaggtcg gccatggcca
     4141 ggttggcaat ctcgctggcg gcggcatccg cgtcggtgcc cggctcgaag tacaccttga
     4201 tcaggctcac cccggtcagg gagcgcgatt cgatgtgatc gatattgctt cccagggtga
     4261 agaagcgctc aaaggtgtcg gtgatgttgg cctcgacctg ctgcggcggc atgccgccgt
     4321 agaaggtggc caccaccacg accggaatgt caatgggagg gaaaagatcg actggcatgc
     4381 gggcgaccgt ggtcacgcca accacgacga tggcgaggca cagcataaga acgaagaagg
     4441 gatacttgat cgcgaaacta ggcattggcc atcactccga atccgcgctg ttggcggcga
     4501 acacagcctc tttcggccgc actaactcgc ctggctgata gctacccagg ttgcccacga
     4561 tgacgcgctc gcctgcagaa aggccggcaa cgatctcgac gttgttggag ctttgcatgc
     4621 cggtttgcac ttcgcgcgcc tgcacccgat tctgcggatc aaccaccaga acactggtct
     4681 tgttctggtc gcgctgaatg gcattgaccg gaacgatcag gacattcttc ctgccgtcgg
     4741 cggccaacac cacatcggca tacatcccgg gctggagatg atagccggga ttgggaacgt
     4801 cgatctccac ctgcatggta cgggtcgaca tatcgagggc gccggtgaag cgggcgacct
     4861 tgccggtgaa atgctcgccc atggcttgga ccttcacatc agcggcatcg ccgatttgca
     4921 ccttggctgc gatcgactcc ggcaccggaa tgcgcagccg caagatattc acctgtgcca
     4981 acttcaccac cggtgcggaa ttgctgttgc tggtgccggc ctgcaccagc gcgccggtat
     5041 cggcatagcg ccaggtgatt acgccatcga aaggcgccgt aatgcgcttg tagtcggaca
     5101 tggcggatac ctggctattg ttggcttggg agacttccag ttgctgtctc gcgccggcca
     5161 gtgccgactt cgctgcctca acctgagctt cggtagcacg gtctttggcc tcggcatcgt
     5221 ccagttcctg ctccgcaatc aaacccggac gggccgtcga tgcctgcttc aga
//
LOCUS       DASRKK010000494         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_626654, whole genome shotgun sequence.
ACCESSION   DASRKK010000494 DASRKK010000000
VERSION     DASRKK010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626654"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFC15_11465"
     CDS             <1..392
                     /locus_tag="VFC15_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10818.1"
                     /translation="ARWFAQVQLPKDFKKEGSTYYNPTTVHRASHQGYKAGQTVFILD
                     DPSGTPWILQAYSLIVDPNLTYDQLKTLDTRLKLPAGWKYRYKVLDKDLEVGAINGTA
                     RIVQDDLQGTYNACFEEGGQSNCNYKP"
     gene            507..1928
                     /locus_tag="VFC15_11470"
     CDS             507..1928
                     /locus_tag="VFC15_11470"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM10819.1"
                     /translation="MKFIKGAALCLALLVVTLFVACSSSKETKTLGLEEAVGTAIDAY
                     LYGYPLITFDIARQQQTNVAVPDAEHAPMGQMIRMRTYPAVDNHCCAAPNADTLYTEA
                     WLDVSKEPLVLSIPDMGNRYYIIPMLDGYSEVFSVASPATTGYKAQTYAITGPGWTGT
                     LPPGVTQVKSATGMVWVLGRIYCTGTPEDYTAVHALQDKFSIVPLSSYGKPYTPPPRQ
                     VDSNFDMKTAVRKQVDALDIDAYFTRLAQLMKTNPPTAADATLVARMASIGLIPGQDY
                     DPSKLGAFDREAIKAVPKLALVKMVKLLKEQKTTNGWLYFTSDVGNWGTDYPLRAMGN
                     MLGPGWNRPQDAVYPLSQKDANGDDYNGADHKYVIHFEKGPFPPVKAFWSITLYDPDF
                     FFVPNSINRYELSQRNKFITNKDGSVDMYLQAESPGKGKEANWLPAPKGKFVLVMRLY
                     WPTSTPPSILDGSWTPPAATRAQ"
BASE COUNT          453 a          602 c          593 g          378 t
ORIGIN      
        1 aggcgcgttg gtttgcccag gtgcagttgc caaaggattt caaaaaggaa ggttcgacct
       61 attacaatcc gacgactgtc catcgcgcgt cccatcaggg ctataaagcg ggccagacgg
      121 tcttcattct tgacgacccg agtggcactc catggattct gcaggcctac tcgctaatcg
      181 tcgaccccaa cctgacttac gaccaactca agacgctcga cactcgactc aagcttccgg
      241 ccggttggaa gtaccgctac aaggtgttgg acaaggacct cgaggtcggc gcgatcaacg
      301 gcaccgcacg catcgtccag gacgatctgc aaggaaccta taacgcgtgc tttgaggagg
      361 gtggccagtc caactgcaac tacaagccat agtttcaggg actggtgcgc atcgctcgca
      421 gggcaggctt cctctgccct gtgacccttc gttgagaaga tgcttggggc aacagagtaa
      481 gtcttccgct accggaggta ggactcatga agttcatcaa gggcgcggcc ctatgtctgg
      541 cgctgctagt cgtgacactg ttcgtcgctt gcagttccag caaagagacg aagactctgg
      601 ggctggaaga agcggtcggc acggcgattg acgcgtacct gtacggctat ccgctgatta
      661 cgtttgacat cgcgcgccag cagcagacga acgtagcggt gccggatgcc gagcatgctc
      721 cgatgggaca gatgatccgg atgcgcacct atccggcggt ggacaaccac tgctgcgctg
      781 cgccgaacgc cgacacgctg tacaccgagg catggctgga cgtctcgaag gaaccattgg
      841 tgctgagcat tcccgacatg ggcaaccgct actacatcat tcccatgctg gacgggtatt
      901 cggaggtgtt tagcgtagcc agcccggcga cgaccggcta caaggcgcag acttatgcca
      961 tcactgggcc gggttggacg ggcacgctgc cgcccggagt gacgcaagtc aaatcggcca
     1021 cgggaatggt atgggtactg gggcgcatct actgcaccgg tacacctgag gactacacgg
     1081 cggttcacgc cttgcaggac aaattctcca tcgttccgct cagttcctac ggcaagccct
     1141 acacgccccc gccgcggcag gtggattcca acttcgacat gaagaccgcc gtgcgcaagc
     1201 aagtggacgc gctcgacatc gacgcctatt tcactcgcct ggcgcagttg atgaagacga
     1261 atccaccgac cgccgctgat gctactctgg ttgcgcggat ggccagcatc ggattgatac
     1321 ccggccaaga ctacgatccc agcaagctgg gcgcattcga ccgggaggcg atcaaggccg
     1381 tccccaagct ggcgctggtg aaaatggtca agctcctgaa agagcaaaag acaaccaatg
     1441 gctggctgta cttcacttcg gacgtaggca actggggcac cgattatccg ttgcgcgcca
     1501 tgggcaatat gctggggcca ggctggaacc gtccgcagga cgcggtgtat ccgctctcgc
     1561 agaaggacgc gaacggcgac gactacaacg gcgcggatca caagtacgtg atccatttcg
     1621 agaagggacc gttcccgccg gtcaaggcct tctggtcgat cacgctgtac gacccagatt
     1681 ttttcttcgt gccgaactct atcaaccgct acgagctgag ccagcgcaac aagttcatca
     1741 ccaacaaaga cggctcggtg gacatgtatc tccaggcgga atcgccggga aaaggcaaag
     1801 aggccaactg gctgcctgcg ccgaaaggga aattcgtttt agtgatgcgc ttgtactggc
     1861 cgacatccac accgccgtcg atcctggacg gaagctggac tccgccggcc gcgacacggg
     1921 cgcagtaggc cctgctttca ggagcctgcg aattatccgt cgaagcaacc agcgaaaatc
     1981 tggccaatgt ccgtgtagtc ggctaaccgg aagttatccc gaaacc
//
LOCUS       DASRKK010000495         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1213665, whole genome shotgun sequence.
ACCESSION   DASRKK010000495 DASRKK010000000
VERSION     DASRKK010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213665"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFC15_11475"
     CDS             <1..262
                     /locus_tag="VFC15_11475"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZM10820.1"
                     /translation="QPAHLVIHDTNICNERCVKEYGNPCKNFCPANVYEMVEATDTPS
                     GLQIHLNSSNCVHCKTCDIMDPYEIITWVPPEGGGGPNYEGM"
     regulatory      523..667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            837..1874
                     /gene="moaA"
                     /locus_tag="VFC15_11480"
     CDS             837..1874
                     /gene="moaA"
                     /locus_tag="VFC15_11480"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZM10821.1"
                     /translation="MANGRLAKSDWRIAKSVFLIDKFGRRISDLRISITDRCNYRCVY
                     CRSGTDGPQFPEMPIADYLRMARVFVGLGITKIRLTGGEPLLRKGLVEMVRDLGRMRT
                     LEGNQLDIAITTNGHLLAEMAQPLADAGLSRVTVSMDAVDAGEFARITRVPNGYDRVL
                     AGIRAAQRARLSPVKINCVLLRGFNDDQIAAFGRFAREEGVAVRFIEFMPLEEGRVWS
                     PEIVVTLEEIVRRMAEFMPLREIEHVRSETARRYVFEDGIGEIGIIAPVSHPFCGHCS
                     RIRVTSDGKIRTCLFSVREHDLAGLMARGASDEEMVEFILSALEHKEERHHIGEPGFV
                     PASRTMVHIGG"
     gene            2431..3549
                     /gene="nadA"
                     /locus_tag="VFC15_11485"
     CDS             2431..3549
                     /gene="nadA"
                     /locus_tag="VFC15_11485"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZM10822.1"
                     /translation="MATAPITEAVFCSLENYLVLPDSSMDGRIAAAKATLGSECIVLG
                     HHYQRDEVIRFADCRGDSYRLSQMAAQAKGSYIVFCGVHFMAESADILARPGQAVVLP
                     DLNAGCSMADMAEISQVEDAWEQLVNLGLTDDEGSGITPITYMNSTAAIKAFCGERGG
                     MVCTSSNAKGAFGWAFRRNGKILFLPDQHLGRNTGYARGIPLDEMVVWDPYMLQGGQT
                     AERLRKAKVILWKGHCSVHQRFLPEHVDKVRANDPGIQVMVHPECRWEVCQKADGIGS
                     TDYLIKVVQQAPAGSSFAIGTEIHLVNRVAKENPDKRVITLDDSGCLCTTMFRISPQH
                     LCWALENLVDGNVVNQIKVKDNVKKWARVALDRMLEIQ"
BASE COUNT          744 a         1033 c         1039 g          745 t
ORIGIN      
        1 ccagccggcg cacctcgtga tccacgacac caacatctgc aacgagcgct gcgtgaaaga
       61 gtacggcaac ccctgcaaga acttttgccc ggccaacgtt tacgagatgg tcgaagccac
      121 ggacacgccc agcggattgc aaatccatct gaactcttcc aactgcgtgc attgcaagac
      181 ctgcgacatc atggacccgt acgagatcat cacctgggtc ccgccggagg gcgggggcgg
      241 accgaattac gaagggatgt aaccaaggcc gctgttcgct ggtgctattc gctggtggtt
      301 tctgaaagcg cacggcatag caggctgctg acaaaagcga cgttcgggta gtgcggacct
      361 ttaggtccgc gttcagaccg tttgtttttg tcagttgaag tcggcttcag ccgacgacag
      421 tttcggtttt cgggcctttc cggcggcttc ttcaatccct gtttgccgat gagcattttc
      481 cttttctgac tgcagctttc cgccgtctgt ctcatctata atcgctgcta ctgtttccga
      541 cctccgtggc ctaccggaat acccaaggct gcggcggcgg tgttccgcgg tattctgcgg
      601 gaccgagggt gtggcgggaa acggccatgc ctcccgtgct tggaaaggaa acgatcagcc
      661 gcactgtgtc tcttcacgcg ctgtcccgtc ttctttcccg tacaaaaaca gatttgagtt
      721 gcaaagatta gaagccctgc ttgaaaaagg tgcggggaag gtgagtagca gcataggggt
      781 ccttcgactc gctgcgctcg ctcaggatga cagacggaat tgattgctaa gagctaatgg
      841 ctaatggccg tttagcgaaa agcgactggc gaatcgcgaa aagcgtgttc ctcatcgaca
      901 aattcggacg gcgcatcagc gacctgcgga tatccatcac cgatcgctgc aactacaggt
      961 gcgtctattg ccgctcgggc accgacggcc cgcaatttcc cgagatgccg attgctgatt
     1021 acctgcggat ggcgcgcgtc ttcgtcgggc tggggatcac gaagatccgt ctcaccggcg
     1081 gcgagccgtt gttgcgcaag ggcctggtgg agatggtccg cgacctcggc cgcatgcgca
     1141 ctcttgaagg caaccaacta gacatcgcca tcaccaccaa cggacatctg ctggccgaga
     1201 tggcgcaacc gcttgccgat gccggtctga gtcgcgtcac ggtgtcgatg gacgccgtgg
     1261 atgcaggaga attcgcgcgc atcacgcgcg tccccaacgg ctacgaccgc gtgctggccg
     1321 gaatccgcgc cgcgcaacgc gccaggctca gcccagtcaa gatcaattgc gtgctcctgc
     1381 gcggcttcaa cgacgatcaa atcgccgcct tcggccgctt tgcacgcgag gaaggcgttg
     1441 ccgtccgttt catcgaattc atgccgctgg aagaaggacg cgtctggtcg ccggagatcg
     1501 tcgtgaccct cgaagagatc gtgcgccgca tggctgaatt catgccgctg cgcgagattg
     1561 aacatgtgcg cagcgagacc gcccgccgct atgtcttcga ggacggcatc ggcgagatcg
     1621 ggatcatcgc accggtttcg catccgtttt gcggccactg cagccgcatt cgagtcacct
     1681 cggatggcaa gattcgcacc tgcctgttct cggtccgcga gcacgacctg gctggcttga
     1741 tggcgcgggg agccagcgac gaagagatgg tcgagttcat ccttagcgcc ctcgagcaca
     1801 aagaagagcg ccaccacatc ggcgagccag gctttgtgcc cgcgtccagg acaatggtgc
     1861 acattggagg atagcgtcag ctatcagccg tcagctttca gtgagtctgc attgaggaaa
     1921 cgtacgctac caaccttctt ttcccctcgg cttgccccag tcaacctcgg cgtgaacgtt
     1981 ttctggagat accttcgcca gaagtcgcct taacctaggt ttcttcttct tcacaggttt
     2041 gcagttgcgc tgtcgcaaga aacacctcga aagaaagaat aatacacatg gatgcctgtt
     2101 aagcggatga caggctgttc cccttgcatt gacccccctc aacgcgccac aatagattca
     2161 ttcgatcctt gtcacagtgt cgtgtgagca gctaggccac gccgggtgag gcgcgcttta
     2221 tctttttgta actacgcgct gtccaggtca tcgaatgaag tgagtcgtaa agaagcgtca
     2281 acctgtagct tcaacatccc cgtaggtcgg gcgcagtacg cgcagtggat ctactgaagc
     2341 cgtgggccag ctttcaacat ccccgttccg gggcgtgaga ttccgatcga aacggagccg
     2401 cgcggctggg actgaaggca gagacaaaaa gtggctacag caccgattac tgaagcggtt
     2461 ttctgctcgc tcgaaaacta tctcgtactc cccgatagct ccatggacgg ccgcattgcg
     2521 gctgccaaag ctactttggg cagcgagtgc atcgttcttg ggcatcacta tcaacgcgat
     2581 gaagtcatcc gcttcgccga ttgccgtggc gattcctatc gcctttcgca gatggcggcg
     2641 caggccaagg gcagctacat cgtcttctgc ggcgtgcact tcatggcgga aagcgccgat
     2701 atattggcgc gtccggggca agcggtcgtc ctgcccgatc tgaacgccgg ttgctccatg
     2761 gccgacatgg ccgagatttc acaggtggaa gacgcatggg agcagcttgt caatcttggc
     2821 ctcaccgacg acgaaggaag cggcatcacc ccgatcacct acatgaactc taccgcggcc
     2881 attaaggcgt tctgcgggga gcgtggaggc atggtctgca cttcgtctaa cgcgaagggg
     2941 gcgttcgggt gggcgttccg tcgcaacggc aaaatcctct tcctacccga ccagcactta
     3001 ggccgtaata ccggttacgc gcgcggcatt ccgctcgacg agatggtggt gtgggacccc
     3061 tacatgctgc aaggcggcca gactgccgag cggttgcgca aagccaaagt gattttgtgg
     3121 aagggccact gttcggtaca ccagcgtttt ctgccggagc acgtggacaa agtgcgagcc
     3181 aacgatcccg gcatccaggt catggttcat cccgagtgtc gctgggaagt ctgccagaaa
     3241 gccgacggaa tcggctcaac cgactacctg atcaaggttg tgcaacaagc acccgcagga
     3301 tcgtcgttcg ccatcgggac ggagatccac ctggtgaacc gcgtggcgaa ggaaaatccg
     3361 gacaagcgtg tcatcacgct cgacgattcg ggttgtctgt gcaccacaat gttccgcata
     3421 tcgccgcaac acttgtgttg ggcgctggaa aacctggtgg acggcaacgt tgtcaatcag
     3481 atcaaggtaa aagacaacgt caagaagtgg gcacgcgtgg cgcttgatag aatgttagag
     3541 atccagtgat tattcgcaaa c
//
LOCUS       DASRKK010000496         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_626723, whole genome shotgun sequence.
ACCESSION   DASRKK010000496 DASRKK010000000
VERSION     DASRKK010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626723"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFC15_11490"
     CDS             <1..392
                     /locus_tag="VFC15_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10823.1"
                     /translation="ARWFAQVQLPKDFKKEGSTYYNPTTVHRASHQGYKAGQTVFILD
                     DPNGTPWILQAYSLIVDPNLTYDQLKTLDARLKLPAGWKYRYKVLDKDLDVGAINGHA
                     RIVQDDLQGTYNACFEEGGQSNCNYKP"
     gene            459..1247
                     /locus_tag="VFC15_11495"
     CDS             459..1247
                     /locus_tag="VFC15_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10824.1"
                     /translation="MKQKVLFNLVLVVMLTAVCGAAFAQTAEQNMAAQSKQHIIPGKQ
                     LHAVGPNYEFCEVAPIVGTSKENAVANFYNPTGVDHCSPEQFTQIVGDKEKIIKDAGA
                     INVFLNPSRHWTWDEFWVYEVGDEQQFGPVKMVWMGVVPLEAMEAAVGAGHYHPGQIH
                     RNNAYLYKKGTQVYLIDMGGGKVLVMQSWTNFVNKGETADNLKDLGSQFKELPAGWKF
                     RTKVLDKDLRVGTPPPNHLAWVTQDEFQNTYQGCGYDKACSYVP"
     gene            1338..2759
                     /locus_tag="VFC15_11500"
     CDS             1338..2759
                     /locus_tag="VFC15_11500"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM10825.1"
                     /translation="MKFIKGALLCLAVSLVVLIVACSSNKESKTPGLEEAVSTAVDAY
                     LYGYPLITFDIARMQQTNVAAPDAEHAPMGQMIRMRTYPAVDNHCCAAPNADTLYTEA
                     WLDVSKEPSVLSIPDMGNRYYIIPMLDGYSEVFSVASPAITGYKAQTYAITGPGWTGK
                     LPAGVTQVKSATGMVWVLGRVYCTGMPEDYKAAHALQDKFVLVPLSSYGKPYTPPPAQ
                     VDPNFDMKTAVRKQVDGLDVDAYFTRLAQLMKTNPPTAADAPLVARMASIGLVPGQDY
                     DPSKLGAFDREAIKAVPKLALLKMVKLLKEQKTTNGWLYFTSGVGNWGTDYPLRAMGN
                     MLGPGWNRPQDAVYPLSQKDANGDDYNGADHKYVIHFEKGQFPPVKAFWSITLYDPDF
                     FFVPNSINRYELSRRNKFITNKDGSVDMYLQAESPGKAKEANWLPAPKGKFVLVMRLY
                     WPTSTPPSILDGSWTPPAATRAQ"
     gene            complement(2894..>3420)
                     /locus_tag="VFC15_11505"
     CDS             complement(2894..>3420)
                     /locus_tag="VFC15_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10826.1"
                     /translation="SPDTIKGRRDRAILAILLGCGLRRRELADLEFTHLQRRDEHWAI
                     VDLVGKGGHIRTVPVPDWVKDAVDLWVVAAGISAGRLFRCVCRAGKCWGVGVTERVVW
                     HVVKDYAKKIGLGQVAPHDLRRSCAKLCHTAGGELEQIQFLLGHVSVQTTERYLGCKQ
                     RISGAVNDRIGIEP"
BASE COUNT          771 a         1007 c          981 g          661 t
ORIGIN      
        1 aggcgcgttg gtttgcccag gtgcagttgc caaaggattt caaaaaggaa ggttcgacct
       61 attacaatcc gacgactgtc catcgcgcgt cccatcaggg ctataaagcg ggccagacgg
      121 tcttcattct tgacgacccg aacggcaccc cgtggattct gcaggcctat tcgctcatcg
      181 tcgatcccaa cctgacttac gaccaactca agacgctcga cgctcgactg aagcttccgg
      241 ccggttggaa gtaccgctac aaggtgttgg acaaggacct cgatgttggc gcgatcaacg
      301 gccacgcccg gatcgttcag gacgatctgc aaggaaccta taacgcgtgc ttcgaggagg
      361 gtggccagtc caactgcaac tacaagccgt agattctggt tctcgtaaag aatgagttta
      421 tttaattctt atctgtaaga gaaagaggag atccaatcat gaagcaaaaa gtattgttca
      481 atcttgtgct tgtggtgatg ctgactgcgg tttgtggtgc agcattcgcc cagaccgccg
      541 agcagaacat ggccgcgcag tccaagcagc acatcattcc gggcaagcag cttcacgcgg
      601 tcgggccgaa ctatgagttc tgcgaggtgg ctcctatcgt aggcacatcg aaggaaaatg
      661 ccgtcgcgaa cttctacaac ccgaccggcg tagaccattg cagcccggaa cagttcacac
      721 agatcgtagg ggacaaagag aaaatcatca aggacgcggg cgcaatcaat gttttcctga
      781 atccgagccg tcattggacg tgggatgaat tctgggtcta tgaagtcggc gatgagcagc
      841 agttcggacc ggtcaagatg gtttggatgg gtgtcgtacc cttggaagcg atggaagcgg
      901 cagtcggcgc gggccactac catcccggcc agatccatcg taacaacgcg tacctgtaca
      961 agaagggaac gcaggtttat ctgatcgaca tgggcggcgg gaaggtgctg gtcatgcagt
     1021 cctggaccaa cttcgtcaac aagggcgaga cggcggacaa cctcaaagac ctcggcagcc
     1081 agttcaagga gctgccagcg ggctggaagt ttcgcaccaa ggtgctcgac aaggacctga
     1141 gggtcggaac gccgccgccg aaccacctgg cgtgggtgac gcaggacgag tttcaaaaca
     1201 cctaccaggg ctgcggctat gacaaggctt gcagttacgt cccataagcg cacgaactgg
     1261 cgggcgcgca atgatgtgcg cccgcagccg tgcatcggtt gcgtcgtcag ttcgcttgct
     1321 gccggagatt aggacttatg aaattcatca agggcgcact gttgtgtcta gctgtatcgc
     1381 tcgtggtttt gatcgtcgcc tgcagttcca acaaagagtc gaaaactcca ggcttggaag
     1441 aggcggtcag caccgccgtt gatgcttacc tgtacggcta tccgttgatt acgttcgaca
     1501 ttgcgcgcat gcagcagacc aatgtggccg cgccggatgc cgaacatgct cccatggggc
     1561 aaatgatcag gatgcggacg tatccagccg tggacaacca ctgctgcgcg gcgcccaatg
     1621 ccgacaccct gtacaccgag gcatggctgg acgtctccaa ggagccgtcg gtgctgagca
     1681 ttcctgacat gggtaaccgc tattacatca tccccatgct cgacggctac tccgaggtgt
     1741 tcagcgttgc cagcccggca ataacgggct acaaggccca gacctacgcg atcacgggac
     1801 cgggttggac agggaagttg ccggctggcg tgacgcaagt caagtccgcc accgggatgg
     1861 tgtgggtcct gggccgtgtc tattgcaccg gcatgccgga ggactacaag gcagcccatg
     1921 ccctgcaaga taagttcgtg ctcgttccgc tgagctccta tggcaagcct tacactccgc
     1981 caccggcgca ggtcgatccc aacttcgaca tgaaaaccgc ggtgcgcaaa caggtggacg
     2041 ggctcgacgt cgacgcctac ttcacccgcc ttgcacaact gatgaagaca aatccgccga
     2101 ccgccgctga tgctcccctg gttgcgcgga tggccagcat cggattggta cccggccaag
     2161 actacgatcc cagcaagctg ggcgcattcg accgggaggc gatcaaggcc gtccccaagc
     2221 tggcgctgct gaaaatggtc aagctcctga aagagcaaaa gacaaccaat ggctggctgt
     2281 acttcacttc gggcgtaggc aactggggca ccgattatcc gttgcgcgcc atgggcaata
     2341 tgctggggcc gggctggaac cgtccgcagg acgcggtgta tccgctctcg cagaaagacg
     2401 cgaacggtga cgactacaac ggcgcggatc acaagtacgt gatccatttc gagaagggac
     2461 agttcccgcc ggtcaaggcc ttctggtcga tcaccctgta cgacccggat tttttcttcg
     2521 tgccgaactc tatcaaccgc tacgagctga gccggcgcaa caagttcatc accaacaaag
     2581 acggctcggt ggacatgtat ctccaggcgg aatcgccagg caaagcaaag gaagccaact
     2641 ggctgcctgc acccaaaggg aagtttgttc tggtgatgcg cttgtactgg ccgacatcca
     2701 caccgccgtc gatcttggac ggaagctgga ctccgccggc cgcgacaagg gcgcagtagg
     2761 ccctgctttc aggtggctgc gaattatccg tcgaagcaac cagcgaaaat ctggccaatg
     2821 tccgtgtagt cggcaagtcg ctccctgggt gcggcacctg ctgcgctagt gggtcgccaa
     2881 ttacccctcg gcgctacggt tcgataccga tccggtcgtt gaccgctccg ctgatccgct
     2941 gtttgcaacc aaggtagcgc tccgtagttt gaaccgaaac gtggccgagg aggaattgaa
     3001 tctgttccaa ttctccgccc gctgtgtgac atagtttcgc gcacgatcga cgaaggtcgt
     3061 gtggcgcaac ttggcccaga ccgatctttt tcgcataatc tttgaccaca tgccagacaa
     3121 cgcgctccgt cacgccgacg ccccagcact tgccagcgcg gcagacacag cgaaacaacc
     3181 tcccagctga tatgccggcg gcgacgaccc acagatcaac cgcgtccttg acccaatcgg
     3241 gcaccgggac cgttctgata tgcccaccct tcccaacaag atccacgatc gcccaatgtt
     3301 catcgcgccg ttgcagatgg gtaaactcca aatcggccaa ttccctccgc ctcagcccgc
     3361 agccaagcag gatcgcaaga atcgcccgat ctcgtcggcc cttgatcgtg tcaggcgaag
//
LOCUS       DASRKK010000497         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_431704, whole genome shotgun sequence.
ACCESSION   DASRKK010000497 DASRKK010000000
VERSION     DASRKK010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431704"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFC15_11510"
     CDS             <1..421
                     /locus_tag="VFC15_11510"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZM10827.1"
                     /translation="GSRMPDMADYGIHETEVFQPRPGIGHTRISVEEYLQTSYSPDRE
                     YRDGVVLERNTGDKEHSRLQARLAQYLGRRRKQWNIEVYTELRVRITPGWYPLPDVCI
                     YALPDFEERFPSRPPLLWIEILSPRLCTKTRIGAPVT"
     gene            complement(608..2062)
                     /locus_tag="VFC15_11515"
     CDS             complement(608..2062)
                     /locus_tag="VFC15_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM10828.1"
                     /translation="MVTNRIQHSDKEPVKQGAGAMAPGPNRIGAATPFDFSGKNLTPY
                     GGLLPVATMLNKLGFQALMEEAVMVKRLTKVMSGYQFLLAMVLGLYIGFSRLNQLRFI
                     ARDPMLTGVLKIVQLPPQCTLWRFLAALHGSVAQQILWVQGQMRRRVWEAANVKLKTA
                     TLDTDTTVHTLYGQQMGARKGYNPKNKGKKSYQPILTFLAETREYLAGELRNGDRPTG
                     KQIARHLHSAFQSLPAGVERIFARADSGFYCWEAVEAYQKAGCRFIVVARKTSRLVAA
                     LNQAQWKPSAKTDGDAECEFRYQPDGWGQAFRFVALRYEKKVDELEAGEAEQYQLFET
                     SRYTYRVFVTDLEEPIDLAVWFYNQRAGAENLIKEANNDAGLAAHPSKRFDVNCNHFQ
                     LAMLAYNLNCWLMLFNRETSADTDTLRHTTLATARLRFLFVAAKIWRHAGRTGVSYSD
                     HYEERGLFQQMMDRLRAIAGRGPNFGPVVAAALT"
BASE COUNT          451 a          743 c          655 g          516 t
ORIGIN      
        1 tgggagtcgt atgcctgaca tggcagatta cggtattcat gaaaccgaag tgtttcaacc
       61 caggccgggc atcggccata ccaggatttc cgtggaggag tacctgcaaa catcgtactc
      121 tccggaccgg gaataccgtg atggcgtcgt gctggaacga aacacgggcg acaaagaaca
      181 ttcacggcta caagctcgct tggcgcagta cttggggcgc cgccgaaagc agtggaacat
      241 cgaggtatat acagagcttc gggttcggat cacgccggga tggtatcccc ttcccgacgt
      301 ttgcatctac gcgctccccg atttcgaaga acgatttcct tcccggccgc cgctgttgtg
      361 gatcgagatc ctgtcaccta gattatgcac gaaaacccga atcggcgcac cggtaacgtg
      421 agcagcggtg cggctgccgg ggggctgatc tgaatccact ctgatcgcct gctgattaag
      481 tcaacgaacg cctccgctct ctcatttccc tcgactccca tctctgcatc tcagcaactt
      541 ctccctgcta tgttgtcttc ttgcttgcag ttccgagtcg tgcataaaaa gttatgcacg
      601 gcggtcctca ggtcagcgcg gcggccacca ccggcccgaa gttgggacca cgccccgcta
      661 tcgcgcgcag ccggtccatc atttgctgga ataggccgcg ctcttcgtag tgatcgctgt
      721 agctcacccc ggtccttccc gcatgacgcc agatcttcgc cgctacgaac agaaaccgca
      781 atcgcgccgt cgccaatgtg gtgtgacgca gcgtgtcggt gtctgcactg gtctcccggt
      841 tgaacagcat cagccagcag ttcaggttgt aggccaacat cgccaactga aagtgattgc
      901 agttgacatc gaaccgtttg gaggggtgcg ccgccaggcc cgcgtcgttg ttggcttctt
      961 tgatcaagtt ctccgccccc gcccgctggt tgtaaaacca caccgccaga tcgatcggct
     1021 cctccaggtc ggtcacgaag acccggtagg tataccggct ggtctcgaac aactggtact
     1081 gctcagcctc cccagcctcc agttcgtcca ctttcttctc gtagcgcaga gccacgaagc
     1141 gaaacgcctg gccccatccg tccggctggt agcggaattc gcactcggca tcgccgtcgg
     1201 tctttgccga cggcttccat tgcgcctggt tcaacgctgc caccagccgc gaggtcttgc
     1261 gcgccaccac gatgaaccgg cagcccgcct tttggtaggc ttccactgct tcccaacagt
     1321 agaagcccga atcggcgcgc gcgaagattc tctccacccc cgcgggcagc gactggaaag
     1381 cgctgtgtaa atggcgggcg atctgtttcc ccgtcggccg gtcgccattg cgcaactcgc
     1441 ccgccaggta ttcccgcgtc tccgccagaa acgtcagtat cggctggtag cttttcttcc
     1501 ccttgttctt cgggttgtag cccttgcgcg cacccatctg ctgcccgtac agcgtgtgca
     1561 ccgtcgtatc ggtgtccagt gtcgcggtct tcagcttcac gttcgccgct tcccatacgc
     1621 ggcggcgcat ctggccttgc acccacagaa tctgctgggc cacactcccg tgtagcgctg
     1681 ccagaaagcg ccacagcgtg cactgcggcg gcaactgtac gattttcaaa acgcccgtca
     1741 gcatcggatc gcgcgcaatg aaccgcagtt ggttcaggcg actgaacccg atgtacaggc
     1801 ccaacaccat ggcgagcaag aactggtagc cgctcatcac cttggtgagt cgcttcacca
     1861 taaccgcctc ctccatcagc gcctggaagc ccagcttgtt cagcatggtg gccacgggta
     1921 ggaggcctcc gtacggagtt aggttcttac cggaaaagtc gaagggagtg gcagccccga
     1981 ttctattggg tcccggagcc atcgctccag cgccttgttt aacgggctcc ttgtcgctat
     2041 gctgaattct gttcgtaacc atttgggtga cctccatggg gttggcttgc gaagcttgca
     2101 acttcgccaa gtttcagttt catcccatga taacggggtc cccaaatggt tgggtctcag
     2161 ttcaacgccg gccgcatgcc tgatgcggcg catacgtcag gtggcaccag cgatgggcgg
     2221 tggagaaaat cttaggttct ccctttgaag ccgcggccgc gggtggatta gtagcataac
     2281 tgccctagtt agatgggttg aacaaggtaa ccagccaagg ttaccagggg ttaccagcaa
     2341 ccgccgtagg ggaattacct tagct
//
LOCUS       DASRKK010000498         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1116104, whole genome shotgun sequence.
ACCESSION   DASRKK010000498 DASRKK010000000
VERSION     DASRKK010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116104"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..667)
                     /locus_tag="VFC15_11520"
     CDS             complement(140..667)
                     /locus_tag="VFC15_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10829.1"
                     /translation="MYTPVLAKHSKHFSLEDSLAIGVDRMQRNFGPLKQQVEEWQALQ
                     LSTASAKLLIYQAFIEEESGFPKHLARRVHDLYFEPVHEEFQPRTMWSLSNAFTSAFK
                     ELERRDAEAAESKNYGARREVCNLPRSLHGLQRDCARPHRAQGHGRCLARRPSGEYPG
                     SLHALLFRVLRPSQQ"
     gene            872..1588
                     /locus_tag="VFC15_11525"
     CDS             872..1588
                     /locus_tag="VFC15_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZM10830.1"
                     /translation="MIRTFRPIPLKKIKLVIFDLDGTLVDSELDLATSVNDMLRHFGR
                     KELPIQVIGTYIGDGAPMLIRRALGDPVDRTFLQTALTYFLASYHTHKLDNTCPYEGI
                     REALQQIGGGERKLAVLTNKPVRASQGILAGLGLAESFFQVYGGNSLETKKPDPLGAN
                     TLMREAGATPEETVIVGDSEVDILTARNAGSWSVGVTYGLAPQTLQQVPPDVLVDTPA
                     ELARALTTNPVQKQEKLRQK"
     gene            complement(1936..3174)
                     /locus_tag="VFC15_11530"
     CDS             complement(1936..3174)
                     /locus_tag="VFC15_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM10831.1"
                     /translation="MASAIGLVMTGGGARGAYQAGVLKRIGEIKRVQTHGNPFPIIEG
                     ASAGAINGSALAAGSDDFALVTKVIARLWSGLRPSDIFRCDVLSQTRNSLAWMLDLSF
                     GGFIGGGHARSLLDATPLRRFLNAHVNCDRITDNIKRGHLYALAISATNYSSGKGYLF
                     IQGMKGHPMWNRSRRVTLATKITAEHICASAAIPLIFQPVRLKTARGTAFFGDGCIRL
                     QQPLSPIIRLGAKKIFAIGVRGRHLEHQEEPTDQRDPSLAQVLGVLFNVMFLDHLATD
                     MEHLERLNELLGNGQISQAGIEGCEKMRPLTSLLMTPSVDLSQLAEEHQRDLPYLIQY
                     FVNSLGRDATACSDLMSYLLFASKYTSALIDIGYSDASKRIDEIESFLYTDRDIEEPP
                     VISVGTGKSRRAGSLVVRQN"
     gene            3619..5082
                     /locus_tag="VFC15_11535"
     CDS             3619..5082
                     /locus_tag="VFC15_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZM10832.1"
                     /translation="MWLGLDLGTQSVRAMTVSDMGDVLGQSSQPLTSRREGPRHEQDP
                     EDWWRAVVFVCRAALADLPASAVRGLAVDGTSGTVLLCNTSGKALTPGIMYDDMRAKD
                     EARRANEAGAAVWASLGYRMQPSWALPKLLWLLRQHPSVIPGARLIHQNDFINRRFSG
                     HEVPSDSSNALKTGYDLIREAWPHDVLDALGVPESVLPPVVRSGTQLGTVCAEAAEET
                     GIPAGTPIIAGMTDGCAAQIGAGALEVGNWNSVLGTTLVLKGVTGELIRDPAGVMYSH
                     RSPDGNWLPGGASSTGAAALAKHFPGSNLDALSAQAAEREPASIIAYPLVSRGERFPF
                     LAPDAEGFLLGDPVDEIDLYAALLQGVAFIERLCFDYLDILGAPIGGKLTLTGGAARS
                     RYWCQLRADILRQPIRLLENAEPALGMAILAAAVGRRVSDVAREMVRVRKVIEPRLDR
                     IGRFHESYLRLVEELARRGWLTPAVFEHAKRRAALER"
     gene            complement(5186..>5862)
                     /locus_tag="VFC15_11540"
     CDS             complement(5186..>5862)
                     /locus_tag="VFC15_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10833.1"
                     /translation="RQFYDVKVYLDPPEDLRTEWKIKRDTTKRGYTAEQVRAEMEKRE
                     SDSEKFIRPQREFADIVVGFSSDPGVPAEQADGCLNVRLVLRPTIPHPDLSYLFDITH
                     GSEGGVRMQLGRDCGRPVDFLYVDGGVTHEQSTKLEDAIWQHLPDLRPLRADQFGDYN
                     DRNEVRHSDPLALTQLLLTYHLLRVHSGGKPITFASPVAALSRLKSDPSNTEPHMAPV
                     GSVKEN"
BASE COUNT         1218 a         1697 c         1668 g         1279 t
ORIGIN      
        1 cttcgcgccc agatgaactt cgggggcgct tcggatacag cgacttgaag gaagccgccc
       61 cgggtgatgg aagcctatgg gacttgaatg gaactgatcg gcggccagga gtctacgaac
      121 ctgctgtttt ggattggggt cactgttggc tgggccggag tacgcggaat agcagcgcgt
      181 gcagactgcc tggatattct ccggatggtc gtctcgccag gcacctccca tgcccttggg
      241 ctcgatgtgg tctggcacaa tctcgttgta atccgtgaag gctgcgtggc agattgcaca
      301 cttcccgtct tgctccataa tttttcgatt cagcagcttc cgcatctctg cgctccagct
      361 ccttgaacgc gctggtaaac gcgttcgaca ggctccacat ggtccgcggc tggaattcct
      421 catgcacggg ctcgaagtac agatcatgca ctcgccgcgc gaggtgcttg ggaaacccag
      481 actcctcctc gatgaacgcc tggtagatca gcagttttgc cgaagccgtt gacaactgca
      541 gcgcctgcca ttcttcgact tgctgcttca gcggcccgaa gttcctctgc atccgatcga
      601 caccgatggc caggctgtct tccagtgaga agtgcttcga atgcttcgcg agcacgggag
      661 tgtacatgcc tgagacgatg tttccgaatt gactaggtcg ctcccgacgc catttccatc
      721 aggtcagggt gtattgacgg gcccgcttca cggaaggtga gttagctact tgcgcagttt
      781 actctgaaga ctcgactgtc ggatgagatt ttcaccgtag ccgggcaccg agtgtctctg
      841 ccactgagtc gaatggctat actggaaggc aatgatacgc acttttcggc ccattccctt
      901 gaagaagatc aagctcgtca tcttcgatct cgatggcacg cttgtcgatt ccgaactcga
      961 ccttgccacc tcggtgaatg acatgttgcg ccactttggc cgcaaggagc tgccaatcca
     1021 ggtcatcggg acgtacatcg gagacggagc accgatgctg atccgtcggg cgctcggcga
     1081 cccagttgac aggacgtttc tgcagaccgc gctgacctac tttcttgcct cctatcacac
     1141 tcacaagctt gacaacactt gtccttacga aggcattagg gaagcgttac aacagatcgg
     1201 cggaggcgag cgcaagctgg cggtgctcac caacaagccg gtgagggcat cccagggcat
     1261 tctggcgggt ttgggactgg ccgaaagctt cttccaggtt tacggcggca acagcttaga
     1321 aaccaagaag cctgatcccc tgggcgcgaa caccctgatg cgcgaagctg gtgcgacgcc
     1381 ggaagaaacg gtgatcgtgg gcgactccga agtggacatc cttaccgctc gcaatgccgg
     1441 atcgtggagc gtcggcgtta cctacggcct tgcccctcag acattgcaac aggttcctcc
     1501 ggacgtacta gtggacacac cggcggagct ggcccgagcg ctaacgacaa accccgtgca
     1561 gaaacaggaa aagctgcggc agaaatagtc gcactggttt catgatcgtg tgccgcagcc
     1621 tcccgcgcat ttgcagaata ggttttcata ctgtcgctca cgaagactct gttcaccttg
     1681 tcgcagatgt tgttccccga ggtgaaccgc gaagctcaaa cagctttcgg acctgcttct
     1741 ggtgaaggta acgcgaggat tttgtgttcg tcgggcagtg tctcccaagg gtaggtccgc
     1801 gaagaagcag atcgaagagg ctcagactct tttcctgcaa cgagtccaga ggtgactcaa
     1861 gccatctcca gctcggactt gaattggcaa ctcgctctat caaccagaat gatcagcagc
     1921 cgaaaatgga ggcgtttaat tctggcgaac caccaacgag cccgcccgtc tcgatttacc
     1981 cgttcctacg ctgatgaccg gaggctcttc gatgtcccta tcagtataga gaaagctttc
     2041 gatctcatcg attcgcttgc tggcgtcact gtaaccaatg tcaatcagag cactcgtgta
     2101 ttttgatgca aataacaagt aactcataag atccgaacac gcagtcgcgt ctcgtcccaa
     2161 actgtttaca aagtactgga taagataagg caagtctctc tggtgttcct ctgccaattg
     2221 tgataaatcg accgaaggcg tcatgagaag cgatgttaaa ggccgcatct tttcacatcc
     2281 ttcgatacca gcctggctga tctggccatt gcccagcagt tcattcaacc gctcaaggtg
     2341 ctccatgtcc gtagcgaggt gatccaaaaa cataacatta aaaagcaccc ccaagacttg
     2401 cgctagcgaa ggatctctct ggtccgtggg ttcttcctga tgttcgagat gccggccgcg
     2461 gacaccgatc gcgaaaatct tctttgcccc caggcgtatt atcgggctga gaggctgttg
     2521 aaggcggatg caaccatcgc caaaaaacgc cgttcctcgc gccgttttaa gtctgacagg
     2581 ctgaaagatc aaaggaatgg cagccgaagc acaaatgtgt tcggcggtaa tttttgtcgc
     2641 gagtgtcact cgccggcttc ggttccacat agggtggcct ttcattcctt gaatgaagag
     2701 gtaacctttc cccgagctat agttggtcgc cgaaatagca agtgcgtaga gatgcccacg
     2761 cttgatattg tcggtgattc ggtcgcaatt tacgtgtgcg ttgaggaagc gtctcaaagg
     2821 ggtcgcatct agtaaagatc gggcgtgacc tcctccgata aatcctccaa aggagagatc
     2881 tagcatccaa gcaagggaat tacgggtctg tgataggaca tcacagcgga agatatcgga
     2941 aggtctgagg cccgaccaaa gccgggcgat aactttggtc accaaagcaa aatcgtcgct
     3001 accagcggcg agagcagaac cgttaatcgc tccggcggaa gcgccctcta ttataggaaa
     3061 aggattgccg tgcgtttgca cccgttttat ctcgccgata cgtttgagaa cgccggcttg
     3121 ataagcgccg cgagcacccc cgcctgtcat cactagcccg atcgcacttg ccatcgtcta
     3181 ccccctttct ggctagccgt gtgaggatcc ggattgtcgg gcgggtcttg aatgagcggc
     3241 cgtcctggtt ccgttcccgg tttcgacgaa tatgatgtgg caggcctacg tctaagctgc
     3301 cacaccttag gatgtttgta tgtgatgcaa ggcacgaacg gtcgttcgct tgccgccata
     3361 ctcatgtgat gcccttcact aacctttcaa ccgctacaag cgcaataccg gtcatctgct
     3421 gggcaggttc ccactcttct ccgaacaggg tttcactgcc ggcatgacct gcttcggctc
     3481 gcaggggcgc atttgagctc gaatgtttct gggccagtgg gagcaggcgt atattgaact
     3541 tccccggccc ggctcggaat cgttgttgcc agcacatgga tcgggcaacc gcctgggcat
     3601 ttcgaagcga aaggaaatat gtggcttggc ctcgacctcg gtacgcaaag tgtccgcgct
     3661 atgacagtgt cggacatggg cgatgtcctt ggacagagct ctcagccact taccagccga
     3721 cgcgaaggtc cgcggcacga gcaggatccg gaggattggt ggcgcgccgt cgtcttcgtc
     3781 tgccgtgcgg ctcttgccga cctaccagcc tccgcggtcc gggggttggc cgttgacggc
     3841 acctccggca ctgtccttct ctgcaacact tcgggcaagg cactcacgcc ggggatcatg
     3901 tatgacgaca tgcgtgcaaa ggacgaagcg cgccgagcga atgaggccgg agccgctgtc
     3961 tgggcctccc tcggatatcg catgcagccg tcgtgggctc tgcctaagct gctctggcta
     4021 ctacgccaac atcccagcgt gattcccggt gcacgtctta ttcaccagaa cgatttcatc
     4081 aaccgccggt tctccggaca cgaggtccca tcggattcaa gtaacgcgct taagaccggt
     4141 tacgatctga tccgagaagc atggccgcac gacgttctcg acgcgcttgg agtgcctgag
     4201 agtgtgttac cgcccgtcgt gcgctccggc acgcaactcg gcactgtctg cgcggaggcc
     4261 gcggaagaga ccggtatccc ggcggggacg ccaattattg ctggcatgac cgatggatgc
     4321 gccgcccaga tcggcgccgg agccttggaa gttggcaatt ggaattccgt actcgggaca
     4381 acgctggtac tcaaaggcgt gaccggagaa ctgattcgcg atcccgctgg ggtcatgtac
     4441 tcgcatcgct ctcccgatgg caattggttg cctggcggtg cctccagcac tggcgcagcc
     4501 gctctcgcaa agcactttcc aggaagtaac ctcgatgcgt taagcgcaca agcagccgaa
     4561 cgagaaccgg cgagcatcat tgcctacccg cttgtctccc gcggcgaacg tttcccgttc
     4621 ctcgcccccg atgcggaggg atttctgcta ggcgatccgg tcgacgagat tgatctctac
     4681 gcggcgctgc tacagggtgt ggctttcatc gaacggcttt gtttcgatta cctcgacatt
     4741 ctgggcgcac caatcggcgg aaaactcacc ttgacgggtg gcgcagcccg tagtcgctac
     4801 tggtgtcagc ttcgagccga catcctcagg caacccattc gcctgcttga gaacgccgag
     4861 cccgcgttgg ggatggctat cctggcagcc gccgttggcc gacgcgtttc tgatgttgcc
     4921 agggagatgg tgcgggttcg caaggtgatc gagccccggc tggatcggat cgggcgtttc
     4981 cacgaatcgt accttcggct cgtggaagaa ctggcacgcc gcggatggct aacaccggcg
     5041 gtgtttgagc acgctaagag gcgggccgca ctggaacgat gaccccgcca atcgttcgac
     5101 accggaatca caaagacggg tcccgcttct gaagccttgg cccgacgtta gccgcaggaa
     5161 acaaacggtt ctcagatcct ggctctcaat tttccttgac actaccgacc ggcgccatgt
     5221 gcggctccgt gttcgacgga tctgacttca atcggctgag cgccgccacc ggagatgcaa
     5281 aggtaatggg cttgccaccg ctgtggacgc gcagaaggtg ataggtcagc agtagttgcg
     5341 taagcgccag cggatcgctg tgccgcacct cattgcgatc gttatagtcg ccgaactggt
     5401 cagcgcgcaa cggacgcagg tcgggcaaat gttgccagat cgcatcttcc aactttgtgg
     5461 attgttcgtg ggtaacgccg ccgtccacat agagaaaatc taccgggcgg ccacagtcgc
     5521 ggcccagttg catacggacg ccaccttccg aaccgtgggt gatgtcgaac agatagctaa
     5581 ggtccggatg gggaatcgtg ggacgcaata cgagcctgac gttcaggcac ccatcggcct
     5641 gctcagcggg cacgccgggg tcggaagaga agccgacgac gatatccgcg aactcgcgct
     5701 gcggacgaat gaacttttcc gagtccgatt cccgcttctc catctccgct cgcacctgct
     5761 cggctgtgta gccccgtttg gtagtgtccc gcttgatctt ccactcagtc ctcagatctt
     5821 cgggcgggtc gagatacacc ttcacgtcat agaactgtcg ca
//
LOCUS       DASRKK010000499         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_529874, whole genome shotgun sequence.
ACCESSION   DASRKK010000499 DASRKK010000000
VERSION     DASRKK010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529874"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFC15_11545"
     CDS             <1..340
                     /locus_tag="VFC15_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM10834.1"
                     /translation="QAYSQAIEYYEKAVGLQPENADVRTDLGTAYWYTGNADKAIASF
                     EKALTIRPNHAATLFNLGIVKWQGKKDPKGAIVAWEKLLQTNPDYPQKEQVQMLIERA
                     KMHGTSGNKG"
     gene            complement(657..827)
                     /locus_tag="VFC15_11550"
     CDS             complement(657..827)
                     /locus_tag="VFC15_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10835.1"
                     /translation="MKLVELWWSLELPVDFQAMKNIIGLETSINVTEERMESLWVASV
                     GYVADGLRDECS"
     gene            932..1465
                     /locus_tag="VFC15_11555"
     CDS             932..1465
                     /locus_tag="VFC15_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM10836.1"
                     /translation="MESVLNHECSSKTRQEFLATPELPYHFTDSGLNHVYLVGIRYYK
                     CECGQLLADIPAIKQLLELIARDLVEKSEALAPEEIRFLRKRLRKKQTTLAKELGLRP
                     ETLSRMESGDTRTNERTDKLIRLNYALQSEDSILLEQLRKAVQDMLASWKYSYSPSKI
                     VATVNDNEWEAELCPAA"
     gene            1604..>2691
                     /locus_tag="VFC15_11560"
     CDS             1604..>2691
                     /locus_tag="VFC15_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10837.1"
                     /translation="MKSKNVRAKPKKKTSSSLGTGLFAQPAKRIFILGLLLVVATAVL
                     YYPATHHPFVNYDDDAYVTENVHVQAGLNWDTVEWAFTSYDQANWHPVTWLSHALDCQ
                     LFQLEPAGHHGTNVLLHALNAALLFWILVQATGSAGRSFMVAALFALHPINVESVVWV
                     AERKNLLSMLFFLLALGVYRWYAREPRPGRYAVVALLFALGLMAKPQVITLPFVLLLW
                     DYWPLRRMFADGEFAATGSATVFPARSFSRLVLEKLPLVALSAVSAAITIKAQRVGGG
                     INPELSLSARLANAIVSYARYVGKAVWPTHLAPMYPHPGNSLAKWEVFTALLFLLVVT
                     ALVTAGRRHRYLPVGWLWFLGTLIPMIGL"
BASE COUNT          618 a          744 c          734 g          595 t
ORIGIN      
        1 ccaggcttac tcgcaagcga tcgagtacta cgaaaaagct gtcggccttc aacctgagaa
       61 cgccgatgtc cgcactgacc tcggcaccgc ttactggtac accggcaatg ccgacaaggc
      121 catcgcgagc ttcgaaaaag cactgaccat ccggcccaat cacgcggcta cgctgtttaa
      181 cctcgggatc gtcaagtggc aggggaagaa agatcccaaa ggcgcaatcg tagcctggga
      241 aaaacttttg cagaccaatc ccgattatcc gcagaaggag caggtgcaga tgttaattga
      301 gcgcgcgaag atgcacggaa ctagcgggaa caaagggtaa tacgagtcgc tcaaaagccg
      361 ctgcattagg cgagttccaa aagtctcgat aaaacacctg caccgctcga tacctgagcg
      421 agtttgaata caggttcaac tgctcttttt ggagaggtgt gacagagccg gaccctgatc
      481 agcccgaacc ggtcgggtta gagcagtgcg gctcgaatcc gctgcccgtc gcctttctct
      541 acaatttcaa agtaacttgc gaacttgtca agtattgtat tgacataata tcaagcagtg
      601 tcgtaatatg gacgacagtg aaggaaagat tcccggctag cctgacgagg aagcagctaa
      661 gagcactcgt ctcggaggcc atcagcaaca tagccgacag acgcaaccca gagactttcc
      721 atgcgctctt cggtcacgtt gatcgaggtc tccaaaccga tgatgttctt catggcctgg
      781 aaatcgactg gaagttcgag cgaccaccac aattcaacga gtttcattgg caatggaagt
      841 attacatcgc cactgaaagc gtcgatggcg acgcgataac tgtgatcata gcggtagaca
      901 caattcgcaa ggaatttgag gttgtaacca gatggagagc gtcctgaatc acgaatgcag
      961 ttccaaaacg cgccaagagt tcctagcgac accggagctt ccgtaccact tcactgattc
     1021 aggtctcaac catgtgtacc ttgtgggcat tcgatactac aagtgcgagt gtggccaact
     1081 gttggcggat attccggcca tcaagcagct ccttgaactg atcgcgagag acttggtgga
     1141 gaagtcggaa gcacttgcac cagaggagat tcgctttctc aggaagcgct tacggaagaa
     1201 gcaaaccact cttgccaagg aactcggatt acggccggag accctttcta gaatggagag
     1261 cggggacacg cggaccaacg aacgaaccga caagctgatt cggctaaatt acgcgctcca
     1321 atcagaagac tcgattttgt tggagcagct tcgcaaggcc gtgcaagaca tgctcgcgtc
     1381 ttggaaatac tcttactctc cgagcaagat tgttgcgacc gtgaatgaca acgaatggga
     1441 agccgaactc tgtcctgctg catagaactc tgatcgtatt tccttattgc acatactgcc
     1501 cgtggctccg gcccaaagtg tccccgaagc gactctccag ccgcattaga aaggcccatc
     1561 gaatatactc gtcgcaaatg ggggacgagt gacttcggct ggtttgaaat ccaagaatgt
     1621 ccgtgcaaaa cccaagaaga agacttcgtc gtcgctgggg acgggcctgt tcgctcaacc
     1681 agctaaacga atatttattc tcggtctttt gctggtagtc gctaccgccg ttctctacta
     1741 cccggcgacg catcacccat tcgtcaatta cgacgacgac gcctacgtca cggaaaatgt
     1801 ccacgttcaa gccgggctca actgggacac ggtcgagtgg gctttcacca gctacgatca
     1861 ggccaattgg catcccgtga cctggctttc tcacgccctg gattgccaac tctttcagct
     1921 cgaaccggct ggccatcacg gcacaaatgt actgctccat gcgctgaatg cagcgctgct
     1981 gttctggatc ctggtgcagg cgaccgggtc cgcaggccgc agcttcatgg tggctgcact
     2041 gtttgccttg caccccatca acgtcgaatc tgtagtttgg gttgccgagc gcaagaacct
     2101 cctcagcatg ttgtttttcc tgctggcgct gggagtttac cgatggtatg cgcgcgaacc
     2161 gcgacctggg cgctacgccg tggtcgcatt gttgttcgcg ctgggactca tggccaagcc
     2221 gcaggtgatt acgctgccct tcgttctgct gctttgggat tattggcctt tgcggcggat
     2281 gtttgccgac ggcgagttcg ctgcaacagg atcagcgacc gtcttcccgg caaggagttt
     2341 ttcccgtctg gtgttggaaa agctcccgct ggtcgcgctg tccgcagtta gcgctgcgat
     2401 cacgattaaa gcgcagcggg tgggcggcgg catcaatccg gaattgtcgc tttcggcgag
     2461 gcttgcgaac gcgattgttt cctacgcacg ctacgtgggt aaggcggtct ggccgacgca
     2521 cctcgcgcct atgtatcccc atcccggcaa ctccctggcg aagtgggagg tgttcacagc
     2581 attgcttttc ctgctggtcg tcaccgcgct ggtcaccgcc ggacggcgtc accggtatct
     2641 tccagtggga tggctgtggt tcctggggac cttgataccc atgatcggcc t
//
LOCUS       DASRKK010000500         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_920465, whole genome shotgun sequence.
ACCESSION   DASRKK010000500 DASRKK010000000
VERSION     DASRKK010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920465"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFC15_11565"
     CDS             <1..205
                     /locus_tag="VFC15_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10838.1"
                     /translation="GISHLPVSREFAREWQFNGLPTTRQSDFPEADLWPASIRCREHA
                     KMRPKPNGSLIRDYVRDGDYTSK"
     gene            complement(838..3579)
                     /locus_tag="VFC15_11570"
     CDS             complement(838..3579)
                     /locus_tag="VFC15_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM10839.1"
                     /translation="MIDNVISHYRILGKLGGGGMGVVYKAEDTRLHRFVALKFLPEEF
                     ARDPRSLARFQREAQAASALNHPNICTIYDVGEQDGKAFLAMECLDGMTLKHRIGAKP
                     VDTDVLLELAIEIADALDAAHAKGIVHRDIKPVNIFVTERGHAKILDFGLAKVTPSLS
                     STAQAGAAAQTVTIEERLTSPEATVGTIDYMSPEQVRAKELDARTDLFSFGTVMYEMA
                     TGTLPFRGESTGVIFDSILNRAPVPPMRLNPDLPVELERIIAKCLEKDLNLRYQYAAD
                     VRTDLHRLKRDTDSSRQVPVNAGTSAVPVATQPAHTNSGSAQVAAAKQLKSGLSRVVI
                     AALIILGAAAIGVYSVFHSSADMPFQNFTITQITSSGKSIAAAISPDGKYLLTAVEEN
                     GRQSLWLRNLPTNSDIQVIGPADASYRSLTFSSDGNYIYFLKAESNMGERFDLLRAPV
                     LGGAPQMVVRNDNSGATFSLDGTRMAFVRWNDPEPGKFLVLTANIDGTDEKIIATGPA
                     AFFPNLVAWSPDGNQIALVIPGPGEARVSIQLHDLVSSKVRILARFNDLPLNSIVWLQ
                     DRRGLLAAYQRDIGFVTRSQIGFISNPAGQFHTITKDTNDYQTLTVSGDGKTLATVQQ
                     KAMQTLYVLPATGFAGNPPNPALAQSKDAAMFGWASNGGLYFGDGGNLLRMSVDGSNK
                     TTLLNDPSAQVIRPESCPDGRYIVFVWANHAANKKVNIWRLDTDGSNPKQLTYGTTDI
                     GPACSPDGMWVYYDNLDTLQLFRVPIGGGTPEVVQGTGGLVASTGLGLSPDGKRLVFL
                     ATRNDPKTPIARLVLVPLDAGPKAQVQFFDPDARIATSPQFTPDGKALMYIVREKGTD
                     NLWLHPLDGSPGHQITNFQGDLIQAFQFSLDGKALGVMRTHIESDVVLLHDAGSSLQ"
BASE COUNT          927 a         1225 c         1263 g          938 t
ORIGIN      
        1 tggcatctcc cacttgccgg tatctcggga gtttgcacgc gagtggcagt tcaacggttt
       61 gccgactact cgtcaaagtg acttccccga agcagacctc tggcccgcgt cgatacgctg
      121 tcgtgagcac gccaagatgc gacctaaacc aaacgggtcg ctcatccggg attacgtgcg
      181 agatggcgac tatacgtcca agtgacttcc ccgaaccaga cttctggctc gcgtcgaccg
      241 gagcccgtcc ggacacatac agagccgtgg tcaggattac gtactacttg gcgatttctc
      301 gccagcaaat ctgctgccga ccaagacctc gcaataaagc cacgagttgc caacgggtcg
      361 cttcacctag gcggataggg cgtttcccgt caagttgatc ggggacgaag gtaaacgctg
      421 cgcggcgtat cgatggctgt tcgcgcgccg cttcccaaag cgggcacttt caagaaggcg
      481 acgcgccgtc cgcgaacgcg tgacacacag attggctccg attgcgacgc tgcactgccg
      541 tctcacaaag ggtcctttgt gcaacgaggg ccagccaagc aactgcactc ccttgtgcag
      601 gcccagagcg gagattactt acgcaatggc ctgatcgcaa caactatccc actgacccct
      661 ggtttccgag ggatgtatcg ctcatcggtc tcaatgcctg aggccctttt ggtcgagcgc
      721 tggaactatt gcagcttcgc gtactccgcc ttggcttgct tgtagatggg aatgtcgggg
      781 tcggcgtcct tccagacgcg aagaagtctt ggcccgctgc gggaacggcg gacagcgtca
      841 ttgcagcgac gatcctgcat cgtgtaagag cacgacgtca gactcgatgt gggtacgcat
      901 gacgcccaag gccttgccgt cgagcgagaa ttgaaaggcc tggatcagat cgccttgaaa
      961 gttcgtgatc tggtggccag gggagccatc gagcgggtgc aaccacaggt tgtccgttcc
     1021 cttttcgcga acgatataca tgagtgcttt tccgtcgggc gtgaattgcg gggacgtcgc
     1081 aatacgtgca tcgggatcaa agaattgcac ctgcgccttg ggcccggcat cgagcgggac
     1141 taagacaagt ctcgctattg gcgtttttgg gtcgtttctg gtcgcgagaa atactagacg
     1201 cttgccatcg ggtgagagtc caagccccgt tgaggcgaca agcccaccgg tgccctgcac
     1261 cacctcggga gtgccaccgc caatgggcac tcggaaaagt tgtaaggtat ctaagttatc
     1321 gtagtacacc cacattccgt ccggcgagca ggcgggtcca atatcggttg tcccatacgt
     1381 tagctgcttc ggatttgagc catcagtatc taaacgccag atgttgacct tcttattggc
     1441 ggcgtgatta gcccacacga acacgatata gcgcccgtcg ggacagctct ctggccgaat
     1501 gacttgtgcc gagggatcat tgagaagcgt ggtcttgttg ctgccgtcga ctgacattcg
     1561 cagcaagttg ccaccatcgc cgaagtaaag accgccattg ctcgcccagc cgaacatggc
     1621 tgcgtccttg ctttgcgcga gcgcgggatt tggaggattc ccagcgaagc ccgtagcggg
     1681 caaaacataa agggtttgca tggctttttg ttgtaccgtt gccagtgtct ttccatcccc
     1741 tgacaccgta agtgtctggt agtcattcgt gtctttcgtg atggtatgaa attgccccgc
     1801 cggattggag ataaagccaa tttggctgcg ggtaacaaac ccgatatccc tctgataagc
     1861 ggcgagaagg ccgcgccggt cttgcaacca aacgatgcta ttgagcggca agtcattgaa
     1921 ccgggccaaa attcggactt tcgaggaaac caagtcgtgg agctgaattg aaactctcgc
     1981 ttcgcccgga ccgggaatca ccaaagcgat ctgattgccg tcgggcgacc atgcaacgag
     2041 attcgggaag aaagcagcag gcccggtcgc gatgatcttt tcgtctgtgc catcaatatt
     2101 agccgtgagc actaagaact ttcccggttc tggatcattc cagcgcacga aggccatgcg
     2161 agtaccgtca agcgaaaagg tggctccgga gttgtcgttt cgcacgacca tttgcggcgc
     2221 accgcccaac acaggcgctc gcaagaggtc gaatcgttcg cccatattgg attctgcctt
     2281 gaggaaatag atgtaattgc cgtcggacga aaatgtgagg cttcggtaag aagcatcagc
     2341 gggcccaatc acctgtatgt cactgtttgt cgggaggttg cgcagccaca agctctgcct
     2401 gccattctct tccacggcgg tgaggagata tttcccatcg ggcgagatcg ccgccgctat
     2461 agattttccg ctgctcgtga tctgagtgat cgtaaaattc tggaagggca tgtcagcaga
     2521 actatgaaag acggaataaa cgccgatagc cgctgcaccc agaataatca gggcggcaat
     2581 caccactcgg gacagacccg atttgagctg tttcgcggca gcgacctggg ctgaaccgct
     2641 gttcgtgtga gcaggctggg ttgcgaccgg cacggctgat gtccctgcgt tgactgggac
     2701 ttggcggctg gaatccgtat cacgcttcaa tcggtgcaag tcagtgcgaa cgtctgcggc
     2761 gtactggtag cgcaggttga ggtctttttc cagacacttg gcgatgattc gttccagttc
     2821 gactggcaag tcagggttca atcgcatcgg gggaacgggg gcccggttca ggatggagtc
     2881 aaagatcacg ccagtgctct ccccccggaa cggcaacgtc ccagtcgcca tttcgtacat
     2941 gactgtgccg aacgagaaca aatcagtgcg ggcatccaac tccttggccc gaacctgctc
     3001 tggcgacatg tagtcgatcg tgcccaccgt tgcttcggga ctggtcaggc gctcttcgat
     3061 ggtcacggtt tgtgccgccg cacctgcttg cgccgtgctg ctcaaggagg gtgtcacctt
     3121 cgccaatccg aaatcaagga tcttggcgtg accgcgctcg gttacgaaga tgttgacagg
     3181 cttgatgtcg cggtggacga tccccttggc gtgtgcggcg tcgagtgcgt cggcgatctc
     3241 gatggcgagc tccagcaaga catcggtgtc gacgggcttt gcgccgatac gatgcttcag
     3301 cgtcatgccg tcgagacatt ccatggcgag gaacgccttc ccgtcctgct cgccaacgtc
     3361 gtaaatcgtg caaatattgg gatgattcag tgcggaggca gcctgcgctt cgcgctggaa
     3421 acgggcgagc gaccgcggat cgcgggcaaa ctcttccggc agaaatttga gggcgacaaa
     3481 gcggtgaagc cgggtgtcct cagccttgta gaccacgccc atgccaccgc caccgagctt
     3541 tccaaggatg cggtaatgcg agatgacgtt gtcgatcatt aagggatgta tgaactcgcg
     3601 gtcacatcat cttacgggcg ctgcatttgc cagtcaacga atgacggctc aggcgcgacg
     3661 ttgcaaacgg acaacacggg gcttgggacg ccttatcgcg gcgccggcca gggggcaggg
     3721 tgcgatgaca gcctagaaat tacctacgtg aaatcgccat ttagtacgta atccagacca
     3781 cggctcgcaa ccgtcctacg ccctagtgtt tactgatttc cagtgggtga ctttccactt
     3841 ggacgaatag tcggctatgc gcggaagtgc cagctggtgt caactcccga gataccggcg
     3901 tcgaggagat cgcaggccaa cacccggtag cctggccccg tttcccccaa aacttcgggc
     3961 cccctccaat tcggaccttt ggcgagcaaa ggagcgtacg caccttggac gaagtttctg
     4021 aactcacgat gtctgcgact ccgacgttcg ccttactggt ccgagtgatc taaagcaact
     4081 cgtgcagcaa taattatggg gcacacagcc ttttcgttag gacagtcgca ggtttctggg
     4141 gaggacaccg ccacaaattc cgcttgactc cacggagttc ctcatagatg gccttcagtt
     4201 ccaatcgccc tgcagccgcg acgaccggct gtcgacagac ggcaattgcg tgttgcccac
     4261 ggtgcagtgc cgggctacaa tttcatcgca gcttagacgt tccaatcaag gagagcacat
     4321 gaaaatcaga aagatcgcat tgactctggc gct
//
LOCUS       DASRKK010000501         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1214144, whole genome shotgun sequence.
ACCESSION   DASRKK010000501 DASRKK010000000
VERSION     DASRKK010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214144"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFC15_11575"
     CDS             complement(<1..333)
                     /locus_tag="VFC15_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HZM10840.1"
                     /translation="MSEVVSPIFGSCPLPIFEHKQIVLGHGSGGKLTAELIDKIFRPA
                     FANPILDKLDDQAVVQVNDTRLAFTTDSFVVTPIFFPGGDIGRLAVNGTVNDLAMCGA
                     RPLYLAAAF"
     gene            complement(369..1511)
                     /gene="hypD"
                     /locus_tag="VFC15_11580"
     CDS             complement(369..1511)
                     /gene="hypD"
                     /locus_tag="VFC15_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HZM10841.1"
                     /translation="MKYLDEYRDGRLAGKIVEEIHRIQTKPWVIMEVCGGQTHSIVKH
                     GIDYLLPQEIELVHGPGCPVCVTPLEMIDKAHAIARRPDVIFCSFGDMLRVPGSDGDL
                     FTIKSQGGDVRVVYSPLDCLKISRANPDKKIVFFAIGFETTAPANAMLVWRAQQEGVK
                     NVSILVSHVLVPPAIAAILQSPLNRVQGFLGPGHVCAVMGYREYEPLSARFKVPIVIT
                     GFEPLDILQGILMTIRQLESRRAEVENEYSRILDREGNKPAQKLVFNVFEIADRKWRG
                     VGTIPKSGYKLRCEFREHDAERLFDVKEIDTLEPEVCISGQILSGIKKPHDCPAFGKE
                     CNPQHPLGATMVSAEGACAAYYTYGRHLRRDKHDANLTAISMGGPA"
     gene            complement(1508..1789)
                     /locus_tag="VFC15_11585"
     CDS             complement(1508..1789)
                     /locus_tag="VFC15_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZM10842.1"
                     /translation="MCLAIPGKVVEALDQGGIRMAKVQFGGIVREVCLEYVPETQVGD
                     YVLVHVGFAISRVDEEEAQRTYQLLEELDQLTELNAPGVDEIGDARPKQ"
     gene            complement(1791..>2021)
                     /locus_tag="VFC15_11590"
     CDS             complement(1791..>2021)
                     /locus_tag="VFC15_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10843.1"
                     /translation="AAAIVEVCRSIRQRDQLKRVCLSGGTFQNAYLLRRAVEGLRRDG
                     FEVYLHALVPPNDGGISLGQAVIANEVLRRGD"
BASE COUNT          421 a          627 c          549 g          424 t
ORIGIN      
        1 gaaagccgca gccagataca gcgggcgcgc gccacacatg gccagatcgt ttaccgtgcc
       61 attgactgcc agtcgtccaa tatctccgcc gggaaagaaa atcggcgtga ccacgaacga
      121 atccgtggtg aaagccagcc gggtgtcatt cacctggacc acagcctggt cgtcaagttt
      181 gtcgagaatc gggttggcga atgcaggccg gaagattttg tcgatcaact cagccgtcag
      241 cttgccgccg ctgccgtggc cgagcacgat ctgtttgtgc tcgaagatcg gcaggggaca
      301 ggaaccgaag ataggggata cgacttcgct catagcacct cgagagtgtt gcttagatcc
      361 cggctgtgtt acgccgggcc tcccatggaa atcgccgtca agttcgcatc gtgcttatct
      421 ctcctcaggt gcctgccata ggtgtaatag gcggcacaag cgccttccgc cgaaaccatc
      481 gtcgctccca gaggatgctg cggattacat tccttgccga atgccgggca atcgtgcggc
      541 ttcttgatgc cgctcaggat ttgaccgctg atgcaaactt cgggctcgag ggtatcgatc
      601 tccttcacgt cgaaaagacg ctcggcatca tgctcgcgga actcacaacg caatttgtaa
      661 ccgcttttcg gaatcgtccc tacccctcgc catttgcgat cggcgatttc gaacacattg
      721 aacaccaact tctgcgctgg cttgttccct tcccggtcca ggatgcgcga atattcgttc
      781 tccacttcgg cgcgccggct ctccagctgg cggatggtca tcagtattcc ttgcaaaatg
      841 tccagcggct cgaatcccgt tattacaatg ggaaccttga agcgcgcgct gagcggttcg
      901 tattcccggt atcccatgac cgcgcacacg tgtcccggcc ccagaaatcc ctgcacccgg
      961 ttaagcggag attgcaggat cgcggcgatc gcgggcggta ctagaacatg ggaaaccagg
     1021 atggaaacgt ttttcacgcc ttcctgctgc gctcgccata ccagcatggc attggcgggg
     1081 gcggtggtct cgaaaccgat ggcgaagaag acgatcttct tatcgggatt ggcacgcgaa
     1141 atcttcaggc aatccagcgg cgagtaaaca acgcgaacgt cgccgccttg cgacttgatg
     1201 gtaaacaggt cgccgtccga tccgggcacg cgcagcatgt cgccgaacga acagaagatc
     1261 acgtcggggc gccgggcaat ggcgtgcgct ttgtcaatca tctccagggg cgtcacgcaa
     1321 accgggcagc cggggccatg caccagctca atttcctgcg gcagcaagta gtcgattcca
     1381 tgcttcacga tggaatgcgt ctgtccgccg cagacttcca taatcaccca cggtttagtc
     1441 tggatacggt gaatttcctc gacgatcttg ccggccagcc gtccatcccg gtactcatcc
     1501 aggtacttca ttgcttgggt ctcgcatcgc cgatctcgtc cacgcccggc gcattgagtt
     1561 ccgtcagttg gtccaattct tccagcaact ggtaggtacg ctgcgcctct tcctcatcga
     1621 ccctgctgat ggcgaagccc acgtggacta ggacgtaatc gccgacctgc gtctcgggaa
     1681 cgtactccag gcaaacctcg cgcacgatcc cgccgaactg gactttggcc atgcggatac
     1741 caccctggtc gagtgcctcc accactttgc cgggaatcgc caaacacata tcagtctcct
     1801 cttcgcaaaa cttcattcgc gatgaccgct tggccgagag agattcctcc atcgttcgga
     1861 ggaaccaagg cgtgcagata aacctcgaaa ccatcgcgcc gtaatccttc cacggcgcgg
     1921 cgcagcaggt acgcgttctg gaacgtacca ccgctcaggc acacgcgctt caattggtca
     1981 cgctgacgaa tactgcggca aacttccaca atcgcggcgg c
//
LOCUS       DASRKK010000502         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_822953, whole genome shotgun sequence.
ACCESSION   DASRKK010000502 DASRKK010000000
VERSION     DASRKK010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822953"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFC15_11595"
     CDS             <1..405
                     /locus_tag="VFC15_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10844.1"
                     /translation="STTRGFAEVGEPNVAVCVRPGTEIAFENDVEYYDVWGSSLGHKL
                     GQKIARFRQLNQDPLAHHDALEFPDGQIVLLTRLCEGQHATVLQLPATLPATNVVDEE
                     NRNSIVAYSGGQRGRSEALALMAFPLGPDDCA"
     gene            1138..1341
                     /locus_tag="VFC15_11600"
     CDS             1138..1341
                     /locus_tag="VFC15_11600"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZM10845.1"
                     /translation="MADERDMEERIRERAFHIWTQEGRPEGRDKDHWELAKAQIAAEE
                     RQAAGQGERAPMPAGFEFRSGPI"
     gene            1582..1794
                     /locus_tag="VFC15_11605"
     CDS             1582..1794
                     /locus_tag="VFC15_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZM10846.1"
                     /translation="MSSRLTAGDLQIITRVAVFRGLKPETVQHIIAPATAVVLRPHEL
                     IMRQDDPATAFFIVIDGWVKLYRNNR"
     gene            complement(1799..1948)
                     /locus_tag="VFC15_11610"
     CDS             complement(1799..1948)
                     /locus_tag="VFC15_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10847.1"
                     /translation="MINEARLREVLNQAAIAIEGLYAELKRKPPIEGSDLIKELRQFA
                     KEFSN"
     gene            complement(1976..2215)
                     /locus_tag="VFC15_11615"
                     /pseudo
     CDS             complement(1976..2215)
                     /locus_tag="VFC15_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_199855086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2209..2804
                     /locus_tag="VFC15_11620"
                     /pseudo
     CDS             2209..2804
                     /locus_tag="VFC15_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611908.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(2826..>3106)
                     /locus_tag="VFC15_11625"
     CDS             complement(2826..>3106)
                     /locus_tag="VFC15_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZM10848.1"
                     /translation="APLEWGQALLLVLPVLLIATIPLSIAGWGTRETAMVLAFGYAGL
                     PESDGLIVSVLFGIAQFTAGLLGGAIWILDRGKRRLAVAERSQTKSAP"
BASE COUNT          710 a          836 c          892 g          668 t
ORIGIN      
        1 tctaccaccc gtggcttcgc cgaagttgga gaaccaaacg tggcggtgtg tgttcggccc
       61 ggtacagaga tcgcttttga gaacgacgtt gaatactacg atgtctgggg cagttccctc
      121 ggccacaaac tcgggcagaa aatagctcgc ttccgtcagc tcaaccagga tccgctggcg
      181 catcacgatg ccctggagtt tccggacggg cagatcgtgc tgctgacccg gctgtgcgaa
      241 ggccagcatg cgacggtact gcaattacca gccactctgc ctgcgacgaa tgtggtggac
      301 gaggagaacc gcaattcaat cgtggcatat tctggcggtc aacgtgggag aagcgaagcg
      361 ttggcactga tggccttccc gctagggccg gatgattgcg cctgatcggc aagcagcgcg
      421 aaggtcaata cgaaatagcg cgctgttccg tatcagtacc cgacaagaca accgatgccg
      481 tcagaccatg cggcctcggt tgtttgctgc gggatgtcgt cgttagcgac cgagcatctt
      541 tgatcccgat ccgagcatga ttgggcttag atcgcttcgc gatcgcccga attgcaacct
      601 cgattgcgat gaaaacaacg aggcaggcaa gagcggcatt caaaattaac ttgagccaac
      661 cgtttgcggg cagcatgcaa ggatgaatgt accgcctgca agatcggttc cgttgccacg
      721 cgcacgataa tgtgagaggt ggccaccgcg gtgagttggt aggtgtcccg acttcgccga
      781 caagtggacg cgcctccgaa tacgccgaaa gatggtatcg atgatggcgt ccgtgcgggg
      841 gaggattatc cgagcgagga tattaacccg gacgacattc cgttctaaaa tcgcatttcg
      901 gcaaaccgcg cctgattaat tacgagcggg cgcaggagcg gagatgagag tgggcggtga
      961 ggcgagaaga tgagtattac gtcgccgctt ggattaaggc ggtaacgcaa attcaaaagt
     1021 ttctctgggc cttcactacg tggtcgataa ggactggatt taaatttgat tacgctttgt
     1081 tcaagggagg aactccgcgg gggcatacgg agtttggctc cagagaggag ttgcgtcatg
     1141 gcagacgaaa gagacatgga ggagcgcatt cgcgaacgag cctttcatat ttggactcaa
     1201 gaagggcgac ccgaaggccg ggataaagac cactgggaat tagccaaggc acagatagcc
     1261 gcagaggaac ggcaggccgc cggtcaaggc gagagggcgc cgatgccggc cggctttgaa
     1321 tttagaagtg gcccaattta acttttgcgt gaggaaaccc gttctgcact catccgttgt
     1381 gatggtgggc cgatcttagg aggaccgtca gatgcggctc tatgtccaga cgtggcttcc
     1441 cttactgtca atcagtttta ctgctgcaat ctgctttggc gtagcgtcat tcgttatggg
     1501 acaggcaaaa cctcgtactg gccccttttg agaatgcttg atttgtcgca atgcggcccg
     1561 tgccatccta aggctttacc gatgtcctca agactgacgg ccggcgatct tcagatcatt
     1621 acccgcgtcg ccgtatttcg gggactgaag ccggagactg tccagcacat tatcgcgcct
     1681 gcgacagccg tcgtgttgcg gccacatgag ttgatcatgc gacaggacga tccggcgaca
     1741 gcatttttca tcgtaatcga cggatgggtg aaactttatc gcaataatcg gtagtgtcct
     1801 aatttgaaaa ttctttcgca aattggcgca gttctttgat gaggtcggaa ccttcaattg
     1861 ggggcttccg cttaagttca gcatagagac cctcaatcgc aatcgcggcc tgattaagca
     1921 cttcacgtaa tcttgcttca ttgatcattg ccgaccccta ctttgacgtt tgttctacca
     1981 attcctctag cgaccatagc cgatccgtca cgttcgccgc catggctggc gtgatgcgca
     2041 aggtcttatg gacgcgcacc agattgtagt gcgcgaaatg taaggcgagc gccgcctgca
     2101 agttttcgag cttcttgctg aatgcgttgg tcaaacgggt aaagcgcctc atgctcatcc
     2161 gcatggtcaa gttttgacgc tcgatcatgc tggtcgagat aaggcgctta cggtccgcac
     2221 aagacggtct acaatcggtt cgtgcgctgg agccgtcttg gtgtgttcaa caagattttc
     2281 gccgaactgg cgcgcaaggc tggcaagccg cgccggttga tgatcgacac gacccatctg
     2341 aaagctcatc gcaccgccgc gagtctttta aaaaagggtc tgttcccaga cgtatcgggc
     2401 gcacaaaggg cggcttgaac tcgaaactgc atgccgtgtg cgatggccat gggcgaccgc
     2461 tgatcatgct gctcagcgaa ggccagatga gcgactataa aggcgcggct ctgatgttaa
     2521 ctgccctgcc gaaggccaag gagcttctcg gcgacaaagg ctatgacgcc gactggttcc
     2581 gcaaggctct cgccgagcga ggtatcacag cttgcatccc ttcaaaatcc aaccgaaaaa
     2641 agccgatcga acatgaccgt acgctctacc gccaacgcca caaaatcgag aacatgttcg
     2701 gcaggctcaa ggactggcga cgtgttcata ctcgctacga ccgatgcgct cacacattca
     2761 tgtcagcaat ctgcatcgcc gccatcgtca tcttctggtt ataatcaatg agtcctgacc
     2821 ctagcttaag gagcgctctt agtctgactc cgttccgcga ctgccaagcg gcgtttgccg
     2881 cggtccagaa tccaaatggc gccacccagg aggccggcgg tgaactgggc gatcccgaac
     2941 aacaccgaca caatcaaacc gtcgctctct ggcagtcccg catagccgaa tgccagaacc
     3001 attgcggttt cgcgcgtgcc ccagccggcg atggacagcg ggatggttgc gatcagcaaa
     3061 actggaagca ccagaagcag ggcctggccc cactcaagcg gtgcag
//
LOCUS       DASRKK010000503         5935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_627157, whole genome shotgun sequence.
ACCESSION   DASRKK010000503 DASRKK010000000
VERSION     DASRKK010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627157"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFC15_11630"
     CDS             <1..327
                     /locus_tag="VFC15_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM10849.1"
                     /translation="LRAHGFDVVSAGDGATAMTVVNREKPDAAILDLGLPGGDGFVVL
                     QRLRSLARTVALPVVVVTARPAQTNRALALEQGVVAFLQKPVKTAELMSALRKALRHR
                     EAVSMS"
     gene            complement(445..735)
                     /locus_tag="VFC15_11635"
     CDS             complement(445..735)
                     /locus_tag="VFC15_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10850.1"
                     /translation="MHSFFIALKLGLDLSPRKSFAAGFLFGSLALFGLGTQFSARAFF
                     SSLENETEGYGNRRHEGDGGDVGGPDSDGRPERLLCRFKGEHHAGRAQANCQ"
     gene            730..1710
                     /locus_tag="VFC15_11640"
     CDS             730..1710
                     /locus_tag="VFC15_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM10851.1"
                     /translation="MHLAFEAAKIGFWDWNIVTGETDWSAIASRQMGLPEDSPTSFAI
                     FMNAIHPDDRKALQGAIDGAVQNNQDYAIEYRVLWPDGSLHWRFAKGRAIYDNTGRPV
                     RMAGIAVDIDDRKAVDERLLLQATALQAAANAIVLTDNQGTILRVNPAFTQVTGYSAD
                     EAIGKNPRLLKSGKQDAAFYASLWNTIAAGNIWHGELTNRRKNGGLYAEEMTIAPVRS
                     GAGAISHYVAIKQDITQRKLAEQALQDAEQKYRSIFENAGLGIFQTNLEGEFLSVNPA
                     VARMVGYDSPEDFHDSVHCAAELCVDLKRYDQLLELVRTPTAESPTAESG"
     gene            1927..2550
                     /locus_tag="VFC15_11645"
     CDS             1927..2550
                     /locus_tag="VFC15_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM10852.1"
                     /translation="MKNSKWILVIGFMFLVTIASFGQEYPKVEVPVGFSFVNVHPQVA
                     GISSFNVFGGGGGFVYNFSPLFGVKADFQGYTQSSGQKFYQDGQFLGSVSGNLFTYMF
                     GPQIKKHSGMFQPFGEALFGAAHTNLAANICKLEGGCGSGSGSNNAFAMEFGGGLDIP
                     ITKTIQFRPVEVDYLYTHFGANHVAGVSTHQNNFKYLAGVNFTFGNK"
     gene            complement(2822..3256)
                     /locus_tag="VFC15_11650"
     CDS             complement(2822..3256)
                     /locus_tag="VFC15_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM10853.1"
                     /translation="MNRPGVLKRRPADTACDFLVAVTARFRVLCVFVMMEIGSRRVLH
                     YNVTAHPTAEWTLQQLREAIPSDHPYQFLIHDRDSIFSAGLDEELKSSFGLRVLRTPV
                     RAPKANAYCERLIGTVRRECLDFMIPLNERHLRRSLQCWITL"
     gene            complement(3288..5834)
                     /locus_tag="VFC15_11655"
     CDS             complement(3288..5834)
                     /locus_tag="VFC15_11655"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZM10854.1"
                     /translation="MNIRVRNTLSWTTALLMAVLMTVAFPKRAAADDNDPPGRVARIR
                     YLQGSVSFQPAGETDWAAAVTNRPMTTGDHLWADEGSRAEVQIGSAIIRLDANTGFSF
                     LNLDDRTVQIQLTEGTLNLRVRRLGRDEVFEVDTPNQAFSITQAGQYRVQAGGDGASS
                     LVIVRQGQGEVTGGGRTYSVYSGQSGTFIGTDSLDGQVAAAGNLDDFDTWGQTRDRQY
                     EGSRSARYVSPDVVGYEDLDNNGSWRSDPTYGNVWMPTSVEAGWAPYHNGHWVWISPW
                     GWTWVDDAPWGYAPFHYGRWVSLHGNWGWVPGPISYQPVYAPALVAFIGGPGFGVSIS
                     LGGGGDVGWFPLGPREAYVPGYITSPGYFNRVNTSNTNISNTTITNIYNNQVTNKTTN
                     ITNVTYVNRTVPGAVTAVPQSAFRSSEPVARAAVAVNAKQLASAPVVSRAAVAPTRDS
                     VLGKSAATANRVAQPSAAVANRPVVAKTTPPPPPVSFAKQQQELAKHPGQPLAPHEVQ
                     SLRPPNVAAAHPLVKQAPPGKPTKPGNAPVAINPGQPAHAQPANTPPNKPVNAPPPNK
                     SANAPANQGQPAHTDRPPSAQSNNQPSPGNKPGTMNPSHPANAQPTPAANKPANVPPA
                     QSVAPAPAKQAQPARSDRPPMAQPNNQPPPASQQGRTNANRPANAQPASEPANKPANI
                     PPARPAASSPTSPVPAPENRPVAPVNRSTPPPPASPSVSAPHPPAAPARQPGRPAAPA
                     NRSAPPPPASPSVSAPHPPAAPAQQPGRPAAPANRSTPPPPVSPSVGAPPHPPAAPAQ
                     QPGRPPTPQPSQPPKAQPASPPAAQSNAQKQQAEKAKAEQQKKEQKPPPQ"
BASE COUNT         1142 a         1714 c         1859 g         1220 t
ORIGIN      
        1 ctgcgagcgc acggctttga tgtggtctcg gctggcgacg gggcgacggc aatgaccgtg
       61 gtcaatcggg aaaaacctga tgcagccatt ctcgatctag gtttgcctgg gggcgacgga
      121 tttgtcgtcc tgcaacggct caggtctttg gctcgcacgg tggccctgcc cgtggttgtg
      181 gtgaccgcgc gcccggcaca gacaaaccgg gccttggcac ttgagcaagg cgttgtagcg
      241 ttcttgcaaa aaccggtgaa aaccgcggaa ttgatgagtg cattacggaa ggcgctgcgg
      301 cacagggaag cggtatcgat gtcttgagaa taacagccgt cgccaactcc ggtctggcgc
      361 cggccagagg acagcggata ccgattgtgt cagcgccagt ccgctggtcg aaagtcgcga
      421 atgctaaaga gtcgaagcgc gagattattg gcaattggcc tgagcgcggc ctgcgtgatg
      481 ctcgcctttg aagcggcaaa gcagacgctc cggccggcca tcggaatctg ggcctcccac
      541 atcgccacca tcaccttcat gacggcggtt gccgtagccc tcagtttcgt tctccagcga
      601 cgagaagaac gctcgcgccg agaattgagt accgagtcca aagagcgcca acgagccgaa
      661 gagaaatccc gctgcgaagc tcttgcgcgg ggacagatcg aggcccagct tcagcgcaat
      721 gaagaaagaa tgcacctggc cttcgaggcc gccaaaatcg ggttctggga ctggaatatc
      781 gtgacgggcg agaccgactg gtcagcaatc gccagccgac aaatggggct gccagaggac
      841 tctccgacca gctttgcaat attcatgaac gccatacatc ccgacgatcg caaggcgttg
      901 caaggggcaa tcgacggggc tgtccagaac aaccaggatt acgccattga gtatcgcgta
      961 ctctggccgg acggcagctt gcattggcgg tttgccaaag gtcgcgccat ttatgacaac
     1021 accgggcgcc cggtgcggat ggccgggatc gcggtcgata tcgatgaccg taaagctgtg
     1081 gacgaacgcc tgctattaca ggctacggcg ttgcaagccg ccgccaacgc catcgtgctt
     1141 accgacaacc aaggcaccat actgcgggta aaccccgctt tcactcaggt gacggggtac
     1201 tcggcggacg aagcgattgg aaaaaatccc cggctcttga agtccggtaa gcaggatgcg
     1261 gcgttttatg cgtccttgtg gaacacaatc gcagcaggca acatctggca cggggagctc
     1321 actaaccggc gcaaaaacgg gggcctttat gccgaggaaa tgacgattgc acccgtgcgc
     1381 tccggagcag gcgccatcag ccactacgtc gccatcaagc aggacattac ccagcgaaaa
     1441 ctggccgagc aggcgctaca ggacgccgaa cagaagtacc gcagcatctt cgagaacgcg
     1501 ggcctgggca tctttcaaac caacctggag ggagagtttc tcagcgtgaa tccggctgtc
     1561 gccagaatgg ttggctatga ctcacccgaa gattttcatg acagcgtcca ttgtgcagcc
     1621 gagttgtgcg tcgatctaaa acgctatgac caactgctcg agctggtcag gacacccacg
     1681 gctgagtcac ccacggctga gtcaggttga cgcaaccacc atcagcaact aaaattattc
     1741 cgatatgaac tctgctctct aatgttgttt tagcctgagg ttcctcgcag ttttgccggt
     1801 tcccgccgaa aatacctcgt cggtaacatc caatcaatcc acggggaacc ttggggacgc
     1861 ttccgcgtca ggcgcgtgta cgcagcgagg ggagcagaga atctaaagac tctacaggag
     1921 atagcagtga aaaacagtaa gtggattttg gtaatcgggt tcatgttttt agtaaccatt
     1981 gcctcgtttg gtcaggaata ccccaaagtc gaagtaccgg tggggttctc gtttgttaac
     2041 gtccaccctc aagtggccgg catcagcagt ttcaacgtct tcggtggcgg cggggggttc
     2101 gtttacaact tctcgcctct gttcggggtt aaggccgatt ttcagggcta cacccaaagc
     2161 agcggccaga agttttatca ggacgggcag ttcctgggaa gtgtgagcgg caacctattc
     2221 acctacatgt tcggacctca gatcaagaag cactctggca tgtttcagcc gtttggcgag
     2281 gccctctttg gcgccgcgca taccaatctc gctgcaaaca tctgcaagtt agagggtgga
     2341 tgtggctcgg gcagcgggag caataacgcg ttcgccatgg agttcggtgg cggcttggac
     2401 atcccaatca cgaagaccat tcagttccgt ccggtcgaag tggactatct gtacacccac
     2461 tttggcgcca atcacgtcgc cggcgtctcc acccaccaga acaacttcaa gtaccttgct
     2521 ggcgtgaact tcaccttcgg caacaagtaa gacaactgga ccaagtcgta gcaacactct
     2581 ctggcgtcgc ccctcgggcg acgccagtca tttttgggcc cttgtcctcc gcagaaacct
     2641 tggacggtca cgccgatcgt tgttccaacc aatattcgtg atgtaagccg cccagaacgt
     2701 ccatcactct gatctcgcag tcccgtggca gtctgggcct gtgcgtcgat ggtcgtgggc
     2761 gcggtctgtc cgacgttttc tcaggaaacc ctggccccaa actggagtgc gggcgtcctt
     2821 gttatagtgt gatccagcat tgcaggcttc gccgcagatg ccgctcgttc agcggaatca
     2881 tgaaatccaa gcattcgcgc cgcaccgttc cgatcagccg ttcgcaatac gcgttcgctt
     2941 tcggcgcacg caccggcgtc cgcaataccc tgagcccaaa cgaactcttg agctcctcat
     3001 ccagcccggc tgagaaaatc gaatcgcggt catggatcaa gaactggtaa ggatggtcgc
     3061 tgggaatggc ttctcggagc tgctgcagcg tccactctgc ggtcggatgc gcggtcacgt
     3121 tgtaatgcag aacacgacgg ctcccgatct ccatcatgac gaaaacacag agcacccgga
     3181 atctggccgt gaccgcaacc aggaagtcac aagcggtatc ggccggacgt cttttcagga
     3241 cgcccggccg attcaatgtt cgatgcagca gatcaaaacc gcttccgcta ctgtggtggt
     3301 ggcttctgtt ccttcttctg ctgttcggcc ttggctttct cagcctgctg cttctgtgca
     3361 ttcgattgcg ccgccggtgg cgatgccggt tgcgccttcg gtggttgcga aggttgaggc
     3421 gtcggcggac ggccaggctg ctgcgcggga gcggcgggag gatgcggagg agcgccgacg
     3481 ctcgggctga ccggaggcgg tggggtgctt ctgttagcag gcgctgccgg acggccaggc
     3541 tgctgcgcgg gagcggcggg aggatgcgga gcgctgacgc tcgggctggc cggaggcggt
     3601 ggggcgcttc tgttagcagg cgctgccgga cggccaggct gccgcgcggg agcggcggga
     3661 ggatgcggag cgctgacgct cgggctggcc ggaggcggtg gggtgcttct gttaacaggc
     3721 gccaccggac gattttcagg tgcgggcacg ggacttgttg gcgaactggc ggcagggcgc
     3781 gccggcggta tgttggccgg cttgtttgca ggttcgcttg ccggctgtgc attggccggg
     3841 cggttagcgt tcgtcctccc ctgctggctg gcaggcggcg gttgattgtt cggctgtgcc
     3901 ataggtggcc tatcgctgcg agcaggctga gcctgcttgg ctggtgcggg cgccaccgac
     3961 tgtgcgggcg gtacgttggc cggcttattg gcggccgggg tcggttgagc gttggccgga
     4021 tggctggggt tcattgtgcc cggcttgttg cccggcgaag gttgattgtt cgactgcgcc
     4081 gaaggcggcc ggtcggtgtg cgcaggttga ccctggtttg ccggtgcatt cgcgctcttg
     4141 ttaggaggcg gcgcgttcac aggcttgttc ggcggcgtgt tcgccggctg ggcgtgcgcc
     4201 ggctgtcccg ggttaatagc gactggcgcg tttcccggct ttgttggttt acctggcggc
     4261 gcttgcttga cgagcgggtg cgctgccgca acgtttggcg ggcgcaagct ctgtacttcg
     4321 tgtggagcga gcggctggcc ggggtgcttt gccagctctt gctgttgctt ggcaaaagac
     4381 acaggcggcg gaggcggcgt tgtctttgcc accacgggac ggttggccac cgctgccgat
     4441 ggctgagcca ccctattggc ggttgcggcc gacttcccga gaacactgtc tctggtcgga
     4501 gccaccgccg ctctcgacac aacgggcgcc gaggcaagct gttttgcgtt tacggccacg
     4561 gccgctctcg ccaccggctc tgagctccta aaggcgctct gcgggacggc ggtcactgcg
     4621 ccgggtacgg tcctattcac gtaggtgacg ttcgtgatgt tcgttgtctt gttggtaact
     4681 tgattgttgt agatattggt gatggtcgta ttgctgatgt tggtgttgct ggtgttgacg
     4741 cggttgaagt atccggggct ggtgatgtac ccaggcacat aagcttcccg gggaccgagc
     4801 ggaaaccagc ccacgtcgcc gccgccaccg agcgaaattg acacgccgaa gcccggtccg
     4861 ccaatgaagg ctaccagcgc aggcgcatac acaggttggt agctgatcgg tccaggcacc
     4921 cagccccagt taccatggag agaaacccac cggccatagt ggaacggtgc atagccccaa
     4981 ggagcatcat cgacccacgt ccagccccac ggagagatcc agacccaatg cccattgtga
     5041 taaggggccc atccggcttc aactgaagtg ggcatccaaa cgttgccgta ggttggatcc
     5101 gagcgccagg agccattgtt atccaagtct tcatagccga ccacgtcggg tgagacatac
     5161 cgcgccgatc gcgatccctc atactgacgg tcgcgggttt ggccccaagt gtcgaagtca
     5221 tcgaggttgc cagcggcggc gacctgtccg tccaacgaat ccgtgccgat aaacgtgccg
     5281 ctctgtccgg aataaacgct gtaagtccgg ccaccgccgg tcacttctcc ctggccttgc
     5341 cgcacgatca ccagggaaga ggccccgtct ccgccggcct gaacgcggta ctggcctgcc
     5401 tgcgtaatgg aaaacgcctg attaggcgta tctacttcaa aaacttcgtc gcggcccagc
     5461 cggcgcaccc ggagattgag cgttccctcc gtgagctgta tctgcaccgt gcggtcgtcc
     5521 aggttgagaa aggaaaagcc agtgttggca tccaggcgaa tgatcgccga accaatctgc
     5581 acctccgcgc gtgagccctc gtccgcccaa agatggtcgc ccgtcgtcat ggggcgattg
     5641 gtaacagccg cggcccagtc cgtttccccg gccggctgga aggaaaccga accctggaga
     5701 taccttattc gcgccacacg gcccggcgga tcgttatcgt cagcggccgc ccgcttcggg
     5761 aacgctaccg tcatgaggac agccatcagt aacgcagtcg tccaactcag agtgtttctt
     5821 actcgaatgt tcatagagct attacctgcc tttgggtatt gcaaaaaccg cttctaaggg
     5881 cggtcgcttg agaaatgtga tgcgcccgtg ctgaatgaag ttcgctcgaa cgtcg
//
LOCUS       DASRKK010000504         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_236025, whole genome shotgun sequence.
ACCESSION   DASRKK010000504 DASRKK010000000
VERSION     DASRKK010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236025"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..>2122)
                     /locus_tag="VFC15_11660"
     CDS             complement(294..>2122)
                     /locus_tag="VFC15_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZM10855.1"
                     /translation="AVPRNYQQMLWIWAVSFSLLRPFCIWILGGYRGIWRHFNLNDAL
                     LFALGATPPTVIALCVRLGWSGPIGKVGVPLTVIFADYGVFLMLGLSIRSLRRILYEA
                     SLHGGKRKRTLLAGSEAGLASALRQVALHPEVFVVGLLTPDVKLHVTRISGFEVLGGP
                     IALPKQLVGGEIDLVLVADADPESIGEMIETAMHFGVEVRLLPSAANVISGDVRVSTT
                     PKPEFALEHPVSVVNETHPDVVDTFRGRSVLITGAGGSIGSELSRQVSRLPVAQVLLL
                     DQDENAIFHIHGELSQDSKTKVIPLVADIRDRDRIWSVFAKYRPQVVLHAAAYKHVPM
                     MEYNCTEAVLNNVMGTRTVAEAAIAFAAERFLMISTDKAVNPSSMMGATKRVAELLVQ
                     HLAGRQNGNANLTRCACVRFGNVAGSNGSVVPTFLRQIAMGGPVTVTDAEMTRYFMTI
                     PEAVQLVLQAATLGSNGEVHMLDMGDPVKITTLARRLIEMSGLRPGEDIEIRFIGMRP
                     GEKLQEQLWTDSAVVTPTSFSRVLSIQPPPPTEDFDRYLQSLEAAALTRDDELARKAM
                     MDMPINYVWAQPTGALSNPPASVKPLVMLAPTLLEVAATAKA"
BASE COUNT          479 a          636 c          591 g          416 t
ORIGIN      
        1 gaccctttcg gaagctcgca ccaaaatcgc aaaactcgcc ggcgaagaga ttgcagacgc
       61 gctagccagg cacaatccgg ctgcgatcgt cgaaggcaag agcctgccct accagccgat
      121 gtgccggtga gtttcgcccg cgaggctcac ctgagccatg tgagaagata cagattattg
      181 ccgtcgggac cgcgcagcat cgcagcgcgc tcgccccggg attggccggc agaaagcgac
      241 ttcaatcctg cacatctcac aggctttatc agcaagcggc gtaggccatt tcgtcacgcc
      301 ttggcagtag ctgcaacctc gaggagagtg ggagcaagca taaccaatgg cttaaccgag
      361 gcgggaggat tgctaagagc acctgttggc tgcgcccaga catagttgat cggcatgtcc
      421 atcatagcct tccgcgccaa ttcgtcgtcg cgggtaaggg ctgccgcttc gagcgactgc
      481 agatatctat cgaaatcctc agtcggcgga ggcggctgaa tgctcagtac acgcgagaac
      541 gaagtgggag ttaccaccgc cgaatcagtc caaagctgct cttgcagctt ctccccgggt
      601 cgcataccta tgaaacgaat ctcgatatcc tcaccgggac gcaggccaga catttcgatc
      661 aatcggcggg ccagcgtggt tatcttgact ggatccccca tgtcaagcat gtgaacctca
      721 ccattggagc cgagcgtggc ggcctgaagc accaattgca ccgcctccgg gatggtcatg
      781 aaataacgag tcatctcggc atcggtgacg gtaaccgggc cgcccatggc aatctgccgc
      841 agaaatgtcg gaaccacgct cccgttgctg cccgcgacgt ttccgaaccg cacacaggcg
      901 caacgagtca ggttcgcatt gccgttctgc cggcctgcca agtgttgtac caggagctcg
      961 gccacacgct tggtcgcgcc catcatactg ctgggattca ctgccttgtc agtcgagatc
     1021 atcaggaacc gttccgcagc gaatgcaata gccgcctcgg caaccgtccg ggtacccatc
     1081 acattgttga gcacggcctc ggtgcagttg tactccatca taggcacatg cttgtaggcg
     1141 gcggcatgca acacgacttg cggcctgtac ttggcaaaca cggaccagat gcggtcgcga
     1201 tcgcgaatgt cggcgaccag tggaattacc ttggtcttgc tgtcctggct cagttcgccg
     1261 tgaatgtgga agattgcatt ttcatcttgg tccagcagca ggacttgcgc cacaggaagt
     1321 cgtgacactt ggcggctcaa ctcggagccg atggatccgc ccgctcctgt gatgagcacc
     1381 gagcggccac ggaacgtgtc gactacatcc ggatgagtct cgttgacgac cgaaacaggg
     1441 tgttcaaggg cgaattccgg tttaggggtt gttgacacgc ggacgtcgcc actgatgacg
     1501 tttgcagctg agggtagcag tcgtacttcc acgccgaagt gcatggcagt ttcaatcatc
     1561 tcgccgatcg actccggatc cgcatccgcg acaagaacaa gatcgatttc accgccgacc
     1621 agttgctttg gcagcgctat ggggccgcct aatacctcga aaccactgat ccgggtcacg
     1681 tgcagtttca cgtcgggtgt caagagaccc acaacgaaaa cctcggggtg tagcgccact
     1741 tgccgcagtg ccgatgccag tcctgcttcg gaacctgcca gcaaagtccg ttttcgcttg
     1801 ccgccgtgca gcgatgcctc atacaggatt cttcgcaggc ttcttatact caatccaagc
     1861 atgaggaata cgccatagtc agcgaatatc accgtgagcg gtacacctac tttcccgatc
     1921 ggcccactcc aaccgaggcg cacgcataac gcaatgacgg tgggaggcgt ggcccccagc
     1981 gcgaacagaa gcgcatcatt gagattaaag tggcgccaaa tacctctgta gcctcccaga
     2041 atccatatgc agaatgggcg cagaagcgaa aacgacacag cccaaatcca cagcatctgc
     2101 tgataattac ggggaactgc aa
//
LOCUS       DASRKK010000505         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_41757, whole genome shotgun sequence.
ACCESSION   DASRKK010000505 DASRKK010000000
VERSION     DASRKK010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41757"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFC15_11665"
     CDS             <1..636
                     /locus_tag="VFC15_11665"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HZM10856.1"
                     /translation="QWDEGVRSHEPTEVVGVKNGPPRHHVVAYDYGIKHNIMRRLVGE
                     CCSVTVVPATTSAEDVLALKPDGVFLSNGPGDPEPVTYAQESIRKMMGRVPIFGICLG
                     HQLIGIALGGKTYKLRFGHHGGNHPVKQMKTGKIEITAHNHNFAVDPDSLKQSEVDLT
                     HIDLNDNTLEGLRHRSLPLFSVQYHPEAAPGPHDSHYLFKDFVKMMEEWKK"
     gene            633..1652
                     /locus_tag="VFC15_11670"
     CDS             633..1652
                     /locus_tag="VFC15_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZM10857.1"
                     /translation="MTSRVVGLNGIAKAVHGAFEGFLPAELTHIDDLQTAIPQIARDK
                     ELVAAIARSVRKIPTKHKVLLGDARGMELPDNSVQLVLTSPPYWTLKEYRNTDGQMGH
                     ISPYDEFIEQLDRVWQRCYRSLVPGGRLICVVGDVCLSRRKNDGRHTVVPLHASIQES
                     CRKIGFDNLAPIIWHKIANAAHEVENGGAGFLGKPYEPNAVVKNDIEFILMERKSGGY
                     RTPEPATRILSLIGAEDHKLWFQQIWTGVTGASTRFHPAPYPLVLADRLVRMFSFVGD
                     TVLDPFLGTGTTVVAAALAGRNSIGIEIDSHYLRGAVARIRHETTSLFHQVKIEADEG
                     QQNGV"
     gene            1642..2373
                     /locus_tag="VFC15_11675"
     CDS             1642..2373
                     /locus_tag="VFC15_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HZM10858.1"
                     /translation="MEFSPNLEQRLRSAIQAFWTTRESQAGKQGARSGIRDAGFRAAV
                     TGGAQMNGFISVIRDLLEESGIGKPVIFTERSVELPGWFRPEKKWDLLVVVDNCLIAA
                     IEFKSQVGSFGNNYNNRTEEALGSATDIWAAYREGAFKPSVRPWLGYLMLLEEAPDST
                     RPVRAQEPHFKVFEEFKSASYAKQYEILLTKLVRERLYDSACFLMSSRDSGPSGQYRE
                     PAPELSFENFITSLMARAIACKKSS"
     gene            2380..>2679
                     /locus_tag="VFC15_11680"
     CDS             2380..>2679
                     /locus_tag="VFC15_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10859.1"
                     /translation="MARRNDIAKILIIGSGPIVIGQSAEFDYSGAQACKALKSEGYEV
                     VLANSNPATIMTDPEMADRTYIEPLTRTYLEEIIRIETEMLTASGKPGIFALLPTV"
BASE COUNT          640 a          726 c          746 g          567 t
ORIGIN      
        1 cagtgggacg aaggcgtgcg ctcacacgag ccaacggaag tggtcggtgt aaagaacggg
       61 ccgcccaggc atcacgtcgt cgcctacgac tatggaatca aacacaacat tatgcggcgg
      121 ctggtgggcg agtgctgcag cgtgaccgtg gttcccgcca cgaccagcgc cgaggacgtg
      181 ctcgcgctca agcccgatgg cgtgttcctg tcgaacggcc cgggcgatcc cgagccggtg
      241 acctacgcgc aggagagcat tcgcaagatg atggggcgcg tgcccatctt cggcatctgc
      301 ctcgggcatc agttgattgg catcgcgctg ggtggaaaga cttacaaact gcgcttcggt
      361 caccacggcg gcaatcatcc ggtgaagcag atgaagaccg gcaagatcga gatcaccgcg
      421 cataaccata acttcgcagt cgatcccgat tcactgaaac aaagcgaagt ggatctgacg
      481 cacatcgatc tgaacgacaa caccctggaa ggcctgcggc atcgctcgct acccctgttc
      541 agcgtgcagt atcaccccga ggccgcgccc ggcccccacg actcgcacta cctgttcaag
      601 gattttgtga agatgatgga ggagtggaag aagtgacttc tcgtgtcgtg ggtctgaatg
      661 gcatagcgaa agcggttcat ggcgccttcg aagggttcct gcccgcagag ctaacccaca
      721 tcgacgatct tcaaactgca attcctcaga tcgcgagaga caaggaactg gtcgccgcga
      781 ttgccaggtc ggttagaaag attcccacaa aacacaaggt attgcttggc gatgcccgcg
      841 ggatggagct tccagacaat agtgtccaat tggtcctcac gtcgccgcct tattggactt
      901 tgaaagaata tcggaacacg gacggccaaa tgggccacat ttcgccgtac gacgaattca
      961 tcgaacagct agatcgagta tggcagagat gctaccgttc attggtgcca ggaggaagac
     1021 tgatatgcgt cgtcggcgac gtgtgtctct ctcgcagaaa aaacgatggc cgacacactg
     1081 ttgttcctct gcacgcctcc attcaggaaa gctgtcgcaa gatcggattc gataacttgg
     1141 cgccaattat ttggcacaag attgcaaatg ctgctcacga agtggaaaac ggcggcgccg
     1201 gttttttggg aaaaccttac gaaccaaatg cagtggttaa gaacgacatc gagttcatcc
     1261 tgatggagcg caagtctggg ggatatagaa ctccggaacc tgcgacccgc attctaagcc
     1321 tgatcggtgc cgaagatcac aaactttggt ttcaacagat ctggacagga gtcactggcg
     1381 cttcgacccg cttccatcct gcaccatacc cgctcgtact ggcagatcga ctagttcgaa
     1441 tgttcagttt tgttggcgac acggtgctcg accctttctt gggaacggga acgacagttg
     1501 tcgccgcagc cttggcaggg cgcaacagta tcggcatcga gattgattca cactatcttc
     1561 gtggggcggt agctcgaatt aggcacgaaa caacgtcatt gtttcatcag gtcaagattg
     1621 aagctgatga gggccagcaa aatggagttt agccctaacc tcgagcaaag gctgcgatcg
     1681 gcgatacagg cgttttggac tacgcgtgaa tctcaagccg gcaaacaggg tgcgaggagc
     1741 ggaatccgcg atgcgggttt tcgggccgca gtcacgggcg gagcacagat gaatggtttc
     1801 atctccgtga tccgcgatct gctcgaagaa agcggcattg ggaaaccagt tattttcacg
     1861 gagcgttccg tggagcttcc tgggtggttc agacccgaaa agaaatggga tctcctcgtt
     1921 gtggttgata actgcttgat cgccgcgatc gaattcaagt cacaagttgg atcgtttggg
     1981 aacaactaca acaacagaac tgaagaagcg ttaggcagcg ctacggatat ttgggcggct
     2041 taccgggaag gtgctttcaa gccatcggtc agaccctggc ttgggtacct gatgctactc
     2101 gaagaagcgc cagattctac tcgtcctgtc cgcgctcaag agccccattt caaagtattc
     2161 gaggagttta agagcgcttc ctatgcgaag cagtatgaaa ttttactcac gaagcttgtt
     2221 cgagaacgtc tttacgattc agcttgcttt ctaatgtcaa gtcgtgacag cggcccttcc
     2281 ggccaatacc gtgaaccagc gccagagctt agcttcgaga atttcatcac ttccttgatg
     2341 gccagggcca tcgcttgcaa gaagagttcc tgaaaccaca tggcacgccg caacgacatc
     2401 gcgaagatcc tcatcatcgg ctcggggccc atcgtcatcg gccagtcggc cgagttcgac
     2461 tactctggcg cgcaggcctg caaggccctg aagtctgaag gttacgaagt ggtgctggcc
     2521 aactccaatc cggccaccat catgaccgac ccggagatgg ctgaccgcac ttacatcgag
     2581 ccgctcaccc gtacctatct cgaagagatc attcgaattg aaactgagat gcttactgcc
     2641 agcggcaagc ccggcatctt tgcactctta ccgacggtt
//
LOCUS       DASRKK010000506         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1019281, whole genome shotgun sequence.
ACCESSION   DASRKK010000506 DASRKK010000000
VERSION     DASRKK010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019281"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="VFC15_11685"
     CDS             <1..1942
                     /locus_tag="VFC15_11685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HZM10860.1"
                     /translation="PGSWRKWVPCIVIVPYAICTLGFVAISVGDLTNYSSVEPLYRAL
                     HLIRGAIRLYQYCLVGAFFGLIFTKSAIAISPDAKRRLRLLYWGATTALSPLLLLGVT
                     QWLKESVEFPDWLISIAIVMILIFPLTLAYVIVVQRAMDVRVVIRQGLQYGLAKNGIR
                     TLQAVAILVVILTAFALVGQSRARPQKIIVIAVGILAVFTIRRITERLGAWTDRRFFR
                     EAYDAEQVLSELSDGVRGMVEARSLIETVAARISETLHIPRVAVLLGGVGPYRPAYAL
                     GYGAPPDIAFQLGAGTVKVLQRQQEPARVYFDDPNSWLYREPEVTEEDRSKLTQLDAE
                     LLLPLSARDKLLGFISLGPKRSAEPYSRTDLRLLKSVATQTGLALENAQLISVIADEV
                     AQRERLNREVEIAREVQERLFPQTLPPIVGVDYAGACRSALGVGGDYYDFLALPAGKL
                     GIAIGDVSGKGIAAALTMASLQASLRSEATRAPDNLAGMMSNVNRLVYEASASNRYAT
                     FFYGQYDPASRQLSYVNAGHNPPMLFHCSDGDWPVCRLETGGTVVGLLESFSYQQATL
                     TITPGDILIAFTDGISEAMNDAQDEFGEDRLIETIKSCAGLSPAEIIACIMRAADAFV
                     AGAKQNDDMTLVVLCAQPETGK"
     gene            complement(2666..>3147)
                     /locus_tag="VFC15_11690"
     CDS             complement(2666..>3147)
                     /locus_tag="VFC15_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10861.1"
                     /translation="FFLSYVRQYAYGDVNDAGSYLGNFPSPVIQPTLIASLPSEIPNR
                     FLFWGSYGNLPKKITLSPHIEYRNGFPYQPTNLYQQWVPTTGPQYRYPNYFSLDMRVS
                     KDVQVNPAHAVRLSLTVRNLTNHFNPLEVHSNLADPQYGTFFGNYDRKFLFDFDFLF"
BASE COUNT          642 a          885 c          913 g          707 t
ORIGIN      
        1 gccgggctca tggcggaagt gggtgccatg cattgtgatt gttccttacg ctatttgtac
       61 actcggattt gtagccatct cggtcggtga cctgacaaat tactcctcgg tcgagccgct
      121 ttatcgcgcc cttcatctaa tcaggggtgc catccgcttg taccagtact gtttggtggg
      181 cgctttcttc ggcttgattt tcaccaagtc cgcaatagcc atctctccgg acgcaaaacg
      241 gcgccttcgt cttctctact ggggcgccac tactgccttg tctcccctcc tgctgctcgg
      301 tgtgacgcag tggctgaaag aaagcgttga atttcccgac tggctgatct cgatcgcaat
      361 cgtgatgatc ttgatcttcc cgctcacgct ggcatatgta atcgtggtgc agcgggccat
      421 ggatgtcagg gtagtcatcc ggcagggact gcagtacgga ttggcgaaga atggcatacg
      481 caccctgcaa gcggtcgcaa tcctcgtcgt aattctgact gcctttgcgc tggtaggaca
      541 aagccgtgct cgtccgcaga aaataatcgt gattgcggtt gggatccttg cggtgttcac
      601 catccgccgc ataaccgaaa ggctcggcgc ttggaccgac cggagattct tccgggaagc
      661 ctacgacgcg gaacaagtgt tgagtgaact cagcgatggg gtgcgaggca tggtggaggc
      721 gcgctcattg attgagacag tcgccgcgcg catctctgag acgctgcaca ttccccgggt
      781 cgcagtgttg ctgggaggcg ttgggcctta ccgcccggca tacgctcttg gttatggcgc
      841 acctccggac atagcgttcc agctaggtgc aggcacggtc aaagtgctgc agagacagca
      901 agagcccgcc cgtgtgtact tcgatgatcc caattcctgg ttgtatcgcg aaccggaagt
      961 gacggaagag gatcgctcca agctgacgca gctcgacgcg gaactgctgc ttccgctgag
     1021 tgcgcgggac aaattgctag gattcatcag tctcgggcca aagcgttccg ccgagcccta
     1081 ttcgcgcacg gacctgcgcc ttctcaagtc agtcgcgaca caaaccgggc tggcactgga
     1141 gaatgcccag ctcatctcgg ttattgccga cgaagtggcc cagcgtgaac gcttaaacag
     1201 agaagttgaa atcgcgcgcg aggtgcagga gcgcctcttt cctcagacgc tgccccctat
     1261 cgtcggcgta gattatgctg gcgcctgccg ttcagcgctc ggtgtgggcg gcgattacta
     1321 cgattttctg gccttgcccg cagggaaact cgggatcgcg attggcgatg tttccggcaa
     1381 gggcattgcc gcggcgctga cgatggccag cctacaagcc tctcttcgca gcgaagccac
     1441 ccgggcgccc gacaacctgg cggggatgat gagcaacgtt aaccggctgg tctatgaagc
     1501 gtcggcgtcc aatcgctatg cgactttctt ctacgggcaa tacgatccgg cttcacgcca
     1561 gctcagctat gtgaacgccg gccacaatcc acctatgttg ttccattgtt ccgacggcga
     1621 ctggccggtc tgccggttgg agacgggcgg aacagtcgtg ggtttgctgg agagtttctc
     1681 ctatcagcaa gcgactctga cgatcacgcc gggagacatc ctcatcgctt ttacggacgg
     1741 cattagcgag gccatgaatg acgcgcaaga tgaatttggc gaggaccggc tgatcgaaac
     1801 catcaagagc tgcgcaggct tgagcccggc tgagatcatt gcctgcatca tgcgagccgc
     1861 cgacgcattc gtcgcggggg cgaagcagaa cgatgacatg accctggtcg tgctctgtgc
     1921 ccaaccggaa actggcaaat aggcggccac actgcccgcg gaaaaccttg gactacgcgc
     1981 atcaagtaat tgaccatgcg gttgcctatg tggacggcag ggttcacacg aacgctcttg
     2041 agaacttctg gagcttactg aagcggagta tccacggcac gtatatgagc gtggagccgt
     2101 tccatctcta tcgttacctt gacgaacaat cattccgcta caacctgcgg aagcgaccct
     2161 ctcttttgtt ccgggggcgc cggccaagat cacgattctt cctactggag cgggagatcc
     2221 caaggtagtg cccatacccg gaattgaacg cgtcatagct taccgcgcag gattttttcc
     2281 ggatggcaaa cggatatggt tcgtaggcgc cgagcccggc tcttccaacc gcacgtatac
     2341 cttcgtcccc gatcaactgt cgttcccgat caacttgacg gaaaacgccc taccctttct
     2401 tgtgacttac ggcgtgtcgt ccgctctcag tcgacggttt ggcgactatt cgctcaagtg
     2461 gatttccgcc gcgaaatgcg cccaatagca ttcgtaggcc ctggcgcgtt tatcgttacc
     2521 gatctggcga aacttcctag aacaagactt ccggcccgcg ttgttgagag tacatcccgg
     2581 gcataacttc cgcttagtcc gtgactcggg cgtaatcttc cggaccatgc ataggcaggg
     2641 ttcccttcac tttccacccc cagctttaga acaggaagtc gaagtcgaat aggaacttac
     2701 ggtcgtagtt gccgaagaac gttccatatt gtgggtcggc gaggttggaa tgtacttcca
     2761 gcggattgaa atggttggtc aagttgcgga ccgtcagaga aagtcgtacc gcatgtgccg
     2821 gattgacctg cacgtccttg gacacgcgca tatccagcga gaagtaattg gggtagcggt
     2881 attgaggccc ggtcgtcggc acccactgct gatatagatt cgtcggctgg taaggaaacc
     2941 cgttgcggta ctcgatgtgc ggcgacaacg tgattttctt cggcagatta ccgtagcttc
     3001 cccagaacag gaaacggttg ggtatttcgc tgggtaagct ggcgatcagg gtgggttgaa
     3061 tcaccggcga aggaaaattc cccaggtagg agccggcgtc gttgacgtca ccgtacgcat
     3121 actgtcgcac gtaggacaag aagaact
//
LOCUS       DASRKK010000507         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1019314, whole genome shotgun sequence.
ACCESSION   DASRKK010000507 DASRKK010000000
VERSION     DASRKK010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019314"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /gene="ispF"
                     /locus_tag="VFC15_11695"
     CDS             <1..450
                     /gene="ispF"
                     /locus_tag="VFC15_11695"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZM10862.1"
                     /translation="SHEFRPGIPLKIGGVLIPHDAGLAGHSDGDVLLHALTDALLGAV
                     AAGDIGTYFPPTDPKWKGADSAVFVREALKQIEAAGYAVGNVDSTLILAAPKIGPHAK
                     AMQQHVASLLGVDPSRVGIKAKTPEGMGTDNAAIAHVAVLLNEICWP"
     gene            833..1495
                     /locus_tag="VFC15_11700"
     CDS             833..1495
                     /locus_tag="VFC15_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM10863.1"
                     /translation="MKTHVAGLLLLASCLAFAGQAHADTLYKNGPVNGICDIEGCTVD
                     AWTINFGFSVTNSFTITSPSTIQGFNFAVWLYPGDTLTSVDWAVGTCTFCSDIGNGTA
                     SGASLSSSFMFTNTYGYDIWDVGVTGLNLHVSGGPPDAYWLTLQNAVVPSGDPVYWDE
                     NGGPSTAWTECIHDGQGCIPSESFNIVGTPDTGTTPEPSSIILFGSGILGLAGILRRR
                     ML"
     gene            1656..>2131
                     /locus_tag="VFC15_11705"
     CDS             1656..>2131
                     /locus_tag="VFC15_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZM10864.1"
                     /translation="MTTSNPNADAAATPQADHPSNFIREIILEDLKTNKFAGRVQTRF
                     PPEPNGYLHIGHAKSICLNFGLAAEFGGKTNLRFDDTNPEKEEQEYVDSIITDVRWLG
                     FDWEDRLFYASDYFDQLHAWAVQLINAGKAYVCDLSAEEVRQYRGTLSEPGKESPYR"
BASE COUNT          452 a          625 c          614 g          440 t
ORIGIN      
        1 tcgcacgagt tccggcccgg catcccactg aagatcggcg gggtactgat tccacacgac
       61 gctggtctgg ccggccactc cgatggcgac gtactgctgc acgcgctgac cgacgcgctg
      121 ctgggcgcgg tcgccgccgg agacatcggg acctacttcc cgccaacgga tccgaaatgg
      181 aagggcgctg actcagcggt gttcgtccgc gaggcgctga agcagatcga agcagcgggc
      241 tacgcggtgg gaaacgtgga cagcaccttg attctcgctg cccccaagat tggtccccac
      301 gctaaggcga tgcagcaaca cgtcgcctca ttactcggcg tcgatccctc gcgtgtggga
      361 atcaaagcca agactccgga aggcatgggc actgataatg ctgctatcgc gcacgtggcc
      421 gtgctgctga acgagatttg ctggccgtag gccaggctgc gacgtaacca gcgccttggg
      481 cgcggcagaa gacagcccag cgcttcagcg ctgggtgagc gagtttgatg acccaagtgc
      541 ctccggcacg gcacaattcc gttctcaaaa agaagagtgt gtgccgtccc cgagggactc
      601 atttcattct ctacgctcac ccacccctga aggggtgggc tatcttcctt tcgtcccgca
      661 acgcgggacc ggggtggtta agcgccgggt ggagtgagag atttcagggg aagtcggaat
      721 cagcgatcat gtccggagcg tcgaggaatt gggtgcgctg ctccacaacg gagtcggcca
      781 cgaaacggat tgacaaaggg gatatcctta aaggcacttg ggggatactg gaatgaaaac
      841 gcacgtcgct gggctattgc tgctcgcttc atgtttggca tttgctggtc aggctcatgc
      901 cgacacgctg tacaagaacg gccccgtcaa cgggatttgc gacatcgagg gttgcacggt
      961 cgatgcctgg accatcaact ttggcttttc ggtgaccaac tccttcacga ttacttctcc
     1021 ctcgaccatt cagggtttta acttcgcagt ctggctctac ccgggcgata ccttgacttc
     1081 cgtcgattgg gccgtgggaa cgtgcacgtt ctgctcggac atagggaacg ggacagcatc
     1141 gggtgctagt ctgagttcgt cgtttatgtt caccaacacg tatggctacg acatctggga
     1201 tgtcggcgtc accgggttga atcttcatgt gagcggtggt cctcccgacg cctactggtt
     1261 gaccttgcag aatgccgtgg tgcccagcgg cgatcccgtg tactgggacg aaaacggcgg
     1321 cccgtctacc gcgtggacgg aatgtattca cgatggccag ggctgtatac cctcagaatc
     1381 gttcaacatc gtcggcaccc ctgacacggg cacaacacct gagccaagca gcatcatctt
     1441 gttcggctct ggaattcttg gtctcgctgg aatactccgc cggaggatgc tgtaagtttt
     1501 tcctgtggaa atcaaaccga cccactaccc atcaacccaa tcctcgcgaa tataccgtcg
     1561 cgaccacgta aaatagagct cttggccagt ggtgctcgca ccctgccgcc aacgaatggc
     1621 gacaaggtcg ggcttctctg gactcagaag gagtcatgac tacctcaaac cccaatgcgg
     1681 acgcggctgc cactccgcaa gctgatcatc cgtcaaattt catacgcgaa atcattcttg
     1741 aagatctgaa gaccaacaaa ttcgccggac gcgtgcagac ccgctttccg cctgagccca
     1801 acgggtatct gcacatcggc cacgccaaat ccatctgcct caacttcggg ctggctgcgg
     1861 agtttggcgg caaaaccaac ctacgctttg acgacaccaa tccggaaaaa gaagagcagg
     1921 agtacgttga ctcgatcatc accgatgtcc gctggctggg cttcgattgg gaagaccggc
     1981 tcttctatgc ctcggattat ttcgatcagc tccatgcgtg ggcggtacaa ctgatcaacg
     2041 ccggcaaggc ttacgtgtgc gatttaagcg ccgaggaagt tcgtcagtat cgcggtacac
     2101 tcagcgagcc cgggaaggaa agcccttacc g
//
LOCUS       DASRKK010000508         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_42477, whole genome shotgun sequence.
ACCESSION   DASRKK010000508 DASRKK010000000
VERSION     DASRKK010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42477"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..939)
                     /gene="rsmD"
                     /locus_tag="VFC15_11710"
     CDS             complement(379..939)
                     /gene="rsmD"
                     /locus_tag="VFC15_11710"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZM10865.1"
                     /translation="MRVIAGQFKSRPLRSLAGLDLRPTSDRLRETLFDVLAAAGNLQD
                     SVWLDLYAGTGAVGIEALSRGARQVYFVESAKKHARLLRENLDPLAVPDAFELHERDV
                     AQALPLLESTGVVCDYCFLDPPYRMRGSYERTLGYLSQSRLLQPTSLVIAEHEKKFDP
                     GERFGALVRYRRLDQGDASLSFYRLA"
     gene            1115..2224
                     /locus_tag="VFC15_11715"
     CDS             1115..2224
                     /locus_tag="VFC15_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZM10866.1"
                     /translation="MRSWSIPAGRLFGVDIRIHLTFVFLLAFVWFTQSVAMGTAGAMR
                     GLVLVGIVFGCVVLHELGHAVVARRNGVAVRSIILLPIGGVTLMEDMGQHNADPARDI
                     RISVAGPVVNLVIAAVSGAFILAFLPQVNLWAQPFVHANNLPRSLFWTNVFLGGFNLL
                     PAYPMDGGRILRALLAEHMDYVRATRRAVAIGQGFAMLLIFLGVWNTWLMLIGFFLFV
                     GAQLEDRSVVFQSVLESVRMEDVMLTGFSTLSPADTLEDALQKAVHTLQDDFPVVRGS
                     DMIGVISKQRIVQALRASGNGYVQSAMVRAFEIAQRSDSLASAFRKLGGRGLSMIPVV
                     DQQRLIGIVTMQNLMHSMTLLAETRRLRKQQEAGN"
     gene            complement(2441..3259)
                     /locus_tag="VFC15_11720"
     CDS             complement(2441..3259)
                     /locus_tag="VFC15_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HZM10867.1"
                     /translation="MILRPETKPARTNNAFVSAWEAVARAEKTRSAKYYLVRQPDHAR
                     VSGEIVQQLAIAGAPPVDDDIVRGISLHDEGWAAFDEGRERLQATPANYSSANVALDR
                     EGRPLAFLDIKAGDFLRAWRGSIDSAEAVAPIAALIVSGHFCRLGKFGIGMAVYSEDD
                     ARSVREFLASEEQRQCRLLRLQNRSEGEVQYWTDVLQFCDLLSLYLCCGAQESVEFPQ
                     GIGPNDETIKLQVQDEVFVLTPSPLVKEVEFSVEAHPYPADAGTPSITVAWRVG"
     gene            complement(3265..3693)
                     /locus_tag="VFC15_11725"
     CDS             complement(3265..3693)
                     /locus_tag="VFC15_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZM10868.1"
                     /translation="MTVALKRVYAAPSPQDGARVLVDRLWPRGLKKSDAALDAWLKDL
                     APSNELRKWFHAHPPQWSKFREKYLKELTTEAANTALQQLYELQKKRRRLTLLFASRN
                     EERNNAVVLKQLVDGERKPPTGTGPIRAAASKRGRAARPR"
BASE COUNT          777 a         1179 c         1126 g          811 t
ORIGIN      
        1 gtgaggatta gatgaacgtg atctggcatg aatacaaact cgtgtacctg caatctctgc
       61 ttaacccgat tttcgcgaag tacgtccagc agcagatcgc agagaggatc agattgaagg
      121 atcaatcttc tctgccaagt cgcagacgag gtgaagaatg tacgagtttc ttgcggtgca
      181 agcaacatat gtcgagttga ttatgcgctg tagcgccagt atcgcgaaat cccagggctt
      241 aaagcccgat ggatggaagc gcagctcttt ctcgcaacgc tgaagcgctg cttcccccgc
      301 ataaatgcgg gggctcccac cgaagcaacc atccgcgagg gatctgcttt tccgagggac
      361 ggcacaattc tgagctcttt acgccagccg gtagaagctc agcgatgcgt caccctgatc
      421 cagtctccga tagcgcacca gcgcaccgaa gcgctcacca ggatcgaact tcttttcatg
      481 ctcagcgatg accaggctcg tgggctgtag cagtcgcgat tgcgacagat aaccgagcgt
      541 ccgctcgtag ctgccgcgca tgcggtacgg cgggtcgaga aaacagtagt cgcagaccac
      601 gcccgtactc tccaagagtg gcagcgcttg cgcaacgtcg cgctcgtgca attcaaacgc
      661 atcggggacc gcaagcggat ccagattttc acgcagcagc cgcgcatgct ttttcgccga
      721 ttccacgaaa tacacctggc gcgccccgcg actcagcgcc tcgattccca ccgcgcccgt
      781 cccggcatac aggtcgagcc atacgctgtc ctgtaaattc cctgcggcgg caagcacgtc
      841 aaagagcgtt tcacgcagcc gatcgctggt gggacgcaga tcgagtcccg ctagcgatcg
      901 caggggacgg ctcttaaact gtccggcgat gacgcgcatg gtcaatttcc attcgtgagt
      961 ttcagtcaag cggtcaggac tacagcgaac aactccggga cgtaggccaa cggcgtgagt
     1021 cgccaatcct ttccgctggc tgagtgctga gtgctgagtg ctgaatgttg ctccataaag
     1081 tgtatagaat gaaagtggag gctgttgcgg agcaatgaga agttggtcga ttcccgcggg
     1141 acgattattc ggagtggata tccgcatcca cctcacgttt gtttttctgc tggcctttgt
     1201 ctggttcacc caatcggtgg ccatgggcac cgccggcgcc atgcgcggcc tggtgctggt
     1261 tgggatcgtc ttcggctgcg ttgtactgca tgaactggga cacgcggtag tggcccgccg
     1321 caacggtgtg gccgtccgat ccatcattct gcttcccatc ggcggagtca cgctgatgga
     1381 agacatggga cagcacaacg ccgatcccgc gcgcgacatc cgcatttccg tcgcgggtcc
     1441 agtggtcaat ctggttattg ccgcagtctc cggcgcattc atcctggcat ttctgccgca
     1501 agtgaacctg tgggcacagc ctttcgttca cgccaataac ctgccgcgca gtttgttctg
     1561 gacaaatgtg tttctgggcg gcttcaatct gctgcctgct tatcccatgg atggcggacg
     1621 catcctgcgc gcacttctcg ccgagcatat ggactatgtg cgcgccactc gccgcgcggt
     1681 tgccatcggg cagggattcg ccatgcttct gatcttcctc ggggtatgga acacctggct
     1741 gatgctgatt ggtttctttc tcttcgtggg ggcgcaactc gaagatcgct cggtcgtctt
     1801 ccagtcggtg ctggagagcg tgcgcatgga agatgtaatg ctgaccggct tttccactct
     1861 ttcgcccgcc gataccctgg aggacgcgct gcagaaagcg gtgcacactt tgcaagacga
     1921 cttcccggtg gtccgcggca gcgacatgat cggggtcatc agcaagcagc gtatcgtgca
     1981 ggcactgcgc gccagcggta acggatacgt gcagtcggca atggtacggg ccttcgaaat
     2041 cgcgcaacgc agcgattcgc tcgcgtcggc cttccgcaaa cttggcggac gcggactcag
     2101 catgattccc gtggtcgacc agcaacggct gatcggcatc gtgaccatgc agaacctgat
     2161 gcacagcatg accctgctgg cggagacgcg acggttgcga aagcagcaag aggcggggaa
     2221 ctagcctact gagtgtcgca cagtatagtg acagccttaa agatttgtca tcacgagcgc
     2281 agcgagggat ctgctttttg cccatccacc gagaagcaga ttcctcgcgc ccaaaccccg
     2341 gcgctcgtaa tgacaattca ctctgctcgc tcggttccgt gctcttgcaa tgcgtcacta
     2401 taatttccga cgctcaatag ggcgacactg aagaggggga ctaccccacc cgccacgcta
     2461 ccgtgatgga cggcgtgccc gcatccgccg gatagggatg cgcttcgacg gagaactcaa
     2521 cttccttcac caacggcgac ggcgtcagca cgaaaacctc atcctgtacc tgcagcttga
     2581 tggtctcgtc atttggcccg atgccctgcg gaaattcaac gctctcctgc gctccacaac
     2641 acaaatacag cgaaagcaga tcgcaaaatt gcagcacgtc cgtccagtac tgcacttcgc
     2701 cttcgctgcg gttttgcaaa cgcagcaagc gacattggcg ctgctcttcg gacgccagaa
     2761 attcgcgcac gctgcgcgca tcgtcttccg agtagacggc catgccgatc ccaaacttgc
     2821 ccagccggca gaagtgcccg ctgacgatca gggcggcgat gggggcgacc gcttcggcgg
     2881 aatcaatcga tcctcgccac gcgcgtagga agtcacccgc cttgatgtcg agaaacgcca
     2941 gcggcctgcc ttcacgatcc agcgcaacgt tagcgctgga atagtttgcg ggagtcgcct
     3001 gaaggcgctc acggccttcg tcgaaagcgg cccacccttc atcgtgcagg ctgatgccgc
     3061 gcacgatgtc atcatccacc ggaggcgcgc ccgcgatcgc aagctgctgg actatttcgc
     3121 cagaaacccg cgcgtgatcg ggttggcgca ccagataata cttcgccgat cttgttttct
     3181 cggcgcgggc cacagcctcc caagcggaga caaacgcgtt attggtgcgg gcaggtttgg
     3241 tttcgggccg gaggatcatg gagcctatct cggccgcgcg gcgcgcccgc gcttgcttgc
     3301 agcggcgcga atggggccgg ttccggtggg gggcttgcgt tcgccatcga ccagttgctt
     3361 caacaccacg gcgttattgc gctcttcgtt cctggaggcg aacaacaacg tcaggcggcg
     3421 gcgtttcttc tgtaactcat aaagctgctg caacgctgtg ttcgctgcct cggtagtgag
     3481 ctccttgaga tacttttcgc gaaacttgct ccactgcggt gggtgggcgt gaaaccactt
     3541 gcgcaactca ttcgacggag ccaggtcctt cagccaggca tcgagcgcgg cgtcggactt
     3601 cttcaacccg cgcggccaca atcggtccac caacacacga gcgccatcct gcggcgacgg
     3661 cgcagcgtaa acccgtttga gagcaaccgt catgtgaggt gtattctaac tctgcgggag
     3721 ccgcagtgcg gcctttcgcg aacccagatt tgcccggaca cctggaagaa tgccgagacc
     3781 aaagcgcgcg gggtttgcct tattaactgc aattattctg atcgtgatca cgatctttct
     3841 ggcctgcgat tccgagcctc gcaagagcga cgccgagctc ggactgactc cac
//
LOCUS       DASRKK010000509         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_628104, whole genome shotgun sequence.
ACCESSION   DASRKK010000509 DASRKK010000000
VERSION     DASRKK010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628104"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            791..1960
                     /locus_tag="VFC15_11730"
     CDS             791..1960
                     /locus_tag="VFC15_11730"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM10869.1"
                     /translation="MRIVALDLGAKKTSYCELSQGQVVHRTTVSELSSLQPLLGPDQP
                     AAKVAIEACREAWFVHDLLVEWGNEVVLVDTTRSRQLGIGQHGRKTDRIDAETLARAL
                     EAGRIPVAHVLSPKRRELRRVLGVRRALVESRAQLVTTVRGLVREQGGKIPSCNTEHF
                     VSKVRGQKLVSNIAQLIEPLLVLVHGIDTQLVNVEGQLAALCAQEPIIAALTTTPGVG
                     AVVAATFMSVIDEAKRFQSAHQVESYLGLVPSEDSSGGKRRLGAISKKGNCYLRSLLV
                     QAAWTIVRSSDKSDPLYLWVNQLIQRRGKRIAVVALARRLVGVLWALWRDGTVYDAPH
                     LAHQGARGLRGAVQSLEQQTAAIAQAIKKSSIKHPATSSIATSRRSRKTLAANAA"
BASE COUNT          543 a          687 c          701 g          440 t
ORIGIN      
        1 attgtatccc cagcatctaa ccgaaccatc cctgagcgac gcgcagccat gttcggcgcc
       61 aatcgctaag gaatttgctg aagtaattcc tgatacggaa gtgggcgtac tgctgtcgga
      121 gttgctgccg ttgcctagct ggtattgatt gttcaatccc caacattcaa tcgtcccgtc
      181 actaagcaat gcgcaggaaa atgcacgcga cgctgcgact gcgatggccg ctgtgatacc
      241 ggctacgttc accggcgtag tactgagtgt cgtcgtgcca tcacccagct gaccgcggct
      301 gttgaggccc caacatttga ctgttccccc gctcagcagc gcgcaagaat gctaggcgct
      361 aggaatctca ttgcgccagg caaagtctgg atgaggagga agtgatgtag ataaagctga
      421 aacttcatta gggacccagt gggtaatcaa acccacccag tcaaggttga ccaccagctg
      481 gaagagatgg gatgagtcgg gccgcgaagc tgccaccgtc atgccgacca tcaccaagag
      541 caaagccaac ctgaagtctc gaggcgcggc gcccgtcacg gcgcttgcat ctcgggatcg
      601 agtggacgtc ccgaccgcca agcgacgtgc ccggcgccgg tctggtcaag cagacacgcc
      661 tacgatgccc gttgaggaga acaaagttgc agaaaatagg gccaccctgc cgccgcccgg
      721 cgaatatcct gacagcgccc ctacgcgttt tgaaacgcaa atttcccaag gggccgaagg
      781 caggatgacc atgcgtattg tagcactaga tcttggagca aagaagacca gttactgcga
      841 gctgtcccaa gggcaggtcg ttcaccgcac cacggtcagc gagttgagct cgttgcagcc
      901 gctgctcgga ccggaccaac ccgcagcgaa ggtcgcgatt gaggcttgcc gcgaggcttg
      961 gttcgtgcac gaccttttgg tcgagtgggg caacgaggtt gtgctggtag acaccacgcg
     1021 cagccggcag ttggggattg gccagcatgg gcgcaagacc gatcgcattg atgccgagac
     1081 gctggctcgt gctctggagg ccggcagaat tcccgtggcc cacgttctgt cgccgaaacg
     1141 acgtgagctg cgtcgggtct tgggagtgcg ccgtgccttg gtcgaatcgc gagcgcaatt
     1201 ggtcacgacc gtgcgcgggc tggtgcgtga gcagggcgga aagattccga gctgtaacac
     1261 ggagcacttt gtcagcaagg ttcgggggca aaagctagtg tcgaacatcg cccaactcat
     1321 cgagccactg ctcgtgctgg tgcatggcat tgacactcaa ttggtgaacg ttgaagggca
     1381 attggccgcg ctctgtgcac aagaaccgat catcgccgca ctgaccacaa cgcctggcgt
     1441 gggtgcggtc gtcgccgcta ctttcatgtc cgtgatagat gaagcaaaac gcttccagag
     1501 cgctcaccaa gtcgagtcct atttggggct agtgcccagc gaagacagca gcgggggtaa
     1561 acgccgcctg ggtgcaatct ccaagaaagg caactgctat ttgcgatctc tgttagtaca
     1621 agcagcgtgg acgatcgtac gcagcagcga caagagcgat ccgctgtatc tttgggtgaa
     1681 ccagctcata caacggcgcg gaaaacggat tgcagtcgta gccctggcgc gacgactggt
     1741 gggcgtgctg tgggcattgt ggcgtgacgg cacggtatac gacgcaccgc atcttgctca
     1801 tcagggtgcc cgtggtctac gcggcgccgt tcaaagcctc gagcagcaga cggcggcaat
     1861 tgcacaggcc atcaagaaaa gctcaatcaa gcatccagcc acttcttcta ttgcgacgtc
     1921 tcgtcgctcg cgaaaaactt tggcggccaa cgccgcctga cccaacctgc gctggtaagc
     1981 gtgaaaccta gagcagcaaa gatcggtagg tggatgagcg cgaaagactc gctggcggtc
     2041 ggcttaggtc gacagagccc gtggaagaga ctgcgccgcc accaaccccc ttcggacccc
     2101 ctaatgcacc ggaatcataa tgaccgaacc aggtcggttc cttgagtgcg aatagtcgat
     2161 cgacaccagc tcgttgtttc cctgctcgct cgcgcaatcg caccaaccta attccgaaac
     2221 tcgctgcaga acagcaacag gagagttcta tgccaaaaac cactgtctcg cgaaccgcca
     2281 ccaaaccgcg gaacacaacc agcgaaaaaa gaacgagtaa actgttgacg tctggcccga
     2341 ctcatagtcg agtcttgggc ggcccgacga g
//
LOCUS       DASRKK010000510         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1411185, whole genome shotgun sequence.
ACCESSION   DASRKK010000510 DASRKK010000000
VERSION     DASRKK010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411185"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..828)
                     /locus_tag="VFC15_11735"
     CDS             complement(28..828)
                     /locus_tag="VFC15_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZM10870.1"
                     /translation="MRILFVGDIFGRPGRTIVHERLPELQRQHGVELTIANGENSAGG
                     FGITPQIAEELFELGIDVITTGNHIWDKREIIEYLNSANGNEASLARRVLRPANYPVG
                     SPGFGWYEGRTRSGVAYCVVNLQGRVFMTSNDDPFRFADQLLNRISAKVILVDFHAEA
                     TSEKISLGWYLEGRVTAVLGTHTHVPTADEQVLPGGTAVQTDVGMTGPYDGVIGVQKE
                     QILARFLTNMPARFEAATNDVRLCATLIDCDPTTGRATAVQRIMLRQE"
     gene            complement(1420..>2002)
                     /locus_tag="VFC15_11740"
     CDS             complement(1420..>2002)
                     /locus_tag="VFC15_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM10871.1"
                     /translation="YDADVAKYPHDPARARQILDDAGYPATNGIRFHLTMKTSTDEST
                     RLLAAVLQQQLREAGIALDIRTFEFATFFADVTKGAYQIHSLRWVGGSNLDPDIFEHV
                     FDSGSFAPKRANRTFYSNPRVDNLIREARGTVDQQKRKLIYDEVQRIIAEDLPYVNLW
                     YLDNVLVHTKRVREIELSPSGNYDFLRTAELAR"
BASE COUNT          405 a          561 c          612 g          424 t
ORIGIN      
        1 cggtctacgc caactctgaa actggcgcta ttcctgccgc agcatgattc gctggaccgc
       61 tgttgcccgg ccggtcgtgg gatcgcaatc gatcagggtg gcgcacaagc gcacgtcgtt
      121 cgtcgccgcc tcgaagcggg ctggcatatt agtcagaaag cgagcgagga tttgttcctt
      181 ttgcacgccg atgacgccgt cgtaggggcc ggtcattccc acgtcggtct gcacggctgt
      241 gccgcccggc agcacctgct cgtcggcggt gggcacgtgg gtgtgcgttc cgagcaccgc
      301 tgttactctt ccttcaaggt accagccaag cgaaatcttc tccgacgtgg cttcagcgtg
      361 gaagtccacc aaaattacct tcgcgctgat ccgattgagc agttgatcgg caaagcgaaa
      421 ggggtcgtcg ttgctggtca taaacacacg gccctgaagg ttgacgacgc aataggcgac
      481 accgctgcgc gtgcggcctt cgtaccagcc gaagcccggg gatcccacag gatagtttgc
      541 cggtcgcagg acgcggcgcg ccagggacgc ctcatttccg ttggcggaat tcaggtactc
      601 gatgatctcg cgcttgtccc agatgtggtt gccggtggtg atgacgtcga tgccgagctc
      661 gaacagctct tcggcaattt gcggagtgat gccgaagccg ccggcggagt tttcgccgtt
      721 ggcgatggtg agttcaacgc cgtgttgcct ttgtagttca ggcaggcgct cgtgaacgat
      781 ggtgcgcccg gggcgcccga aaatgtcacc gacgaaaagt atgcgcaagc tgctacagtc
      841 cttgattgac gcggtatttc gatgttatca cgcgtctata gcagtttcac atttggtgta
      901 tagggtggag caccgcttca gctgcattat tcatgaacac tgaggtgtca tcctgagcgc
      961 agcgcccgca aagactttcc gacccgcttc tcttgcgggt cgggcgggcg cggagtcgaa
     1021 ggacccctat agctgcgaga aatcacattg tagccgcgtg gctcagcaac aagtctggga
     1081 ttgctggcgg cgaaagaggc ccctcgactc ctcgctgcgc tcgtccgctc cgtcgaaaaa
     1141 cgtgcccgtg gaaagtgagt gggcagcata ggggtccttc gactcgctgc gctcgctcag
     1201 gatgacaccc aagaaactac gtgactcatg aataatccag gttcagcggt gcagttagga
     1261 ttcggaatga atcggcttca gccactgagg taacaagaca ttacctcagg cgctaaagcg
     1321 cgatccagga actggcggca attatgcacg cctgaaaggc gtgctccacc cgtcgcctat
     1381 acatcaggaa tgaaacagct caaaggattg gggctccgct catcttgcca gctccgccgt
     1441 gcgcaaaaaa tcgtagttgc ccgagggact cagctcgatt tcccgcactc gcttggtgtg
     1501 caccagcacg ttatcgagat accaaagatt gacatacggc aggtcctccg cgatgatgcg
     1561 ctggacctcg tcatagatga gtttgcgttt ctgctggtca acggtgccgc gggcttcacg
     1621 gatcaagttg tccacacgcg gattggaata gaaggtgcgg ttggcgcgtt tgggcgcgaa
     1681 gctgccggaa tcgaagacgt gctcgaagat gtcaggatcg agattgctgc caccgaccca
     1741 acgcaaggaa tgtatctggt aagcgccttt ggttacgtcc gcaaaaaacg ttgcgaattc
     1801 gaaggtgcgg atatcgagcg cgatgccggc ttcgcgcagt tgctgctgca acaccgccgc
     1861 tagcagccgc gtcgactcgt cggtggaagt cttcatcgtg aggtgaaagc gaataccgtt
     1921 ggtcgccggg tagcctgcgt catccagaat ctggcgagcc cgcgccgggt cgtgaggata
     1981 cttcgcaaca tcggcatcgt ag
//
LOCUS       DASRKK010000511         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_335687, whole genome shotgun sequence.
ACCESSION   DASRKK010000511 DASRKK010000000
VERSION     DASRKK010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335687"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFC15_11745"
     CDS             complement(<1..504)
                     /locus_tag="VFC15_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10872.1"
                     /translation="MRYTALHLIMNNQHGFTPAELRKLRSLKDPHGIQRFMDDMPYHL
                     ADTAWSPRRVLRENTAHCFEGAIFAAAALRANGYPPLILDFEAEHDTDHVLAIYRVKG
                     HWGAVAKSNYTGCRYREPVYRSLRELAMSYFNIYFNLRRERTLRTFSRPVNLARFDDQ
                     AWMTTEKP"
     gene            complement(995..2191)
                     /locus_tag="VFC15_11750"
     CDS             complement(995..2191)
                     /locus_tag="VFC15_11750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZM10873.1"
                     /translation="MKKIALFSLSMLVLLAVALPLVAQQRDGSHIFAQPPIRILNPQP
                     DSPGPHGLFPAQIKAAYGFNSTPNQGAGQIIGIVDAYDDPNAESDLQVYDTQFGLPTC
                     TTGNGCFTKIKVGNPPGDSGWGLEISLDIQQAHGLAPAAKVILVEANSNSFADLLAAV
                     DVARQNGATQISMSWSGGEDPSELGFDSFFNHPGVTFMASTGDGGHGVGYPAASPYVV
                     AVGGTTLVLSTATPLPNPLLSNYGHETAWSGSGGGISAYEPGQAYQDGVFAGCGSTGM
                     RCVPDISSDANPGTGVPVYDTYGYGGWIQVGGTSVSSPDWSAVFAVANSSRAAAGKGT
                     LQTAIADLYTFYNSTQYTDFHDITSGTNGSCGAQCTAIVGYDLVTGIGSPKVNKLVPA
                     LVAIEP"
BASE COUNT          476 a          649 c          723 g          451 t
ORIGIN      
        1 cggcttttcc gtcgtcatcc acgcctgatc gtcgaaccgc gcaagattga cgggacgcga
       61 aaaggtccgg agcgtgcgct cgcgccgcaa gttgaaatag atattgaagt agctcatggc
      121 cagctcgcgc aaggaacggt ataccggctc gcgatagcgg cagccggtat agttcgactt
      181 ggccaccgcg ccccaatgtc ccttcacccg gtaaatggca agcacgtggt cggtgtcgtg
      241 ttcagcttca aagtcgagaa tcagcggggg atagccgtta gcccgcaggg cggccgcagc
      301 gaagatcgct ccttcgaagc aatgagcagt gttttcgcgc aacacccgcc gaggcgacca
      361 cgcggtatcg gccaggtggt acggcatgtc atccatgaat cgttgtatgc cgtgggggtc
      421 tttcaaactg cgcagcttgc gcagttccgc cggagtgaag ccgtgttggt tgttcatgat
      481 taagtggaga gcagtgtacc gcatcgcccc tcgctcgagt cttgtgtaca acctcgctac
      541 aagggccggc cagacgcaat ctacgattga agcttgcttc gagaggctgc ggaaaaagtc
      601 ggaaaagcag ggtggagcag cgcttcagcg ctgcatctgg cgatcgctcg ggaaaccggc
      661 tccagccgct gaggtcaatg aagcccatat acctcaggcg ctaaagcacc gatcagaaat
      721 tgccgcccac aatgcacgcc tgaaggcgtg ctccacccgt cgctaataca tcaaaaggtg
      781 aaactgctcc aagccagttg cccaggcaag gtggcagggt gtcatggatg ggcgaaggcg
      841 gaaacggaca cgtcgcgggg cgatccaatg ggtaaggggt ttcagccttg aacgaactga
      901 aaccccttga cccggcgcca cgaaggaagt tcgccgaggt gcgctccccc gtctgagctg
      961 ggagcgcgcc ccgggcagga tggtcttgag taggttacgg ctcgatggcg accaaggccg
     1021 ggaccagctt gttcaccttg ggtgaaccga ttccagtcac caagtcgtag cccacaatag
     1081 ccgtgcactg tgcgccgcag ctgccgttgg ttcccgacgt gatgtcatgg aagtcggtgt
     1141 actgcgtcga attatagaag gtatacaggt cggcgatagc cgtctgaagg gtaccttttc
     1201 ccgccgccgc acggctggaa ttggcgaccg caaagaccgc actccagtcg ggcgaagaca
     1261 cgctggtgcc gccgacttga atccacccac cgtaaccgta ggtgtcatag acgggaacgc
     1321 ccgtgcccgg attggcatcg gaagaaatat cgggaacaca gcgcattcca gtgctgccgc
     1381 agcccgcgaa gactccgtcc tgataggcct ggccgggctc gtaggcgctg attccgccgc
     1441 cgctgccgct ccacgccgtc tcatggccat agttcgacag cagtggattc ggcagtggag
     1501 tagccgtgga cagcaccagc gtggtgccac cgacggccac cacataagga gaggccgcgg
     1561 gatagccgac tccgtgtccg ccgtcgccgg tggaagccat aaaggtgact cccggatggt
     1621 tgaagaagga atcgaatccc agctcgctgg ggtcttcccc accgctccag ctcatggaga
     1681 tttgggtggc gccgttctgg cgggcaacgt ccaccgcggc cagcagatcg gcaaagctgt
     1741 tgctgttggc ttccaccaat atcactttgg ctgccggggc aaggccgtgg gcctgctgga
     1801 tgtccaaaga gatttcgagt ccccaaccgc tgtcgcccgg aggattgccg actttgattt
     1861 tggtgaagca gccgttgccg gtggtgcaag ttggcagacc gaactgagta tcgtaaacct
     1921 ggaggtcgga ttcggcattg ggatcatcgt aggcatccac gatgccgatg atctgccccg
     1981 cgccttggtt gggagtgcta ttgaacccgt aggctgcttt tatctgggct ggaaacaggc
     2041 cgtgcgggcc agggctgtct ggctgcggat tcaggatgcg gattggcggt tgcgcaaaga
     2101 tgtggctgcc atcgcgctgt tgggcgacca agggcaaggc cacagcaagc agaactagca
     2161 tgctgagcga aaacaaagca atttttttca ttcacgtttc cttaggttcg ctgtatcgcc
     2221 tgcgaaaagc gcgattgccg ttgcaggata gcggctctcg ttggctgatt cggatgggaa
     2281 caaatcgacg tcttgctgt
//
LOCUS       DASRKK010000512         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1117559, whole genome shotgun sequence.
ACCESSION   DASRKK010000512 DASRKK010000000
VERSION     DASRKK010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117559"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2268
                     /locus_tag="VFC15_11755"
     CDS             <1..2268
                     /locus_tag="VFC15_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZM10874.1"
                     /translation="RDSPYGGKQESVDVNAEQLQLGDRVLNAAVGIRLTIYSKQTEED
                     EARDEGTGSPLRVYTYAQRLHFIATLRLPRNYRNPGAMDMAGYLASQGIRLTGSSRAS
                     SVQVLPGFVGSRIGLWRSAARRSVLAHIMQRWPGERGALMQAMLIGGRAFFGRDIKTE
                     FQRTGTYHILVVSGINVGILAFTVFWALRKLPFGETWATILTILLSWGYAFLADLGSP
                     IVRATITLGIYLVTRLLFRDRAALNGLGIAALGILLVNPRTLFEASFQLTFLSVIAVA
                     GIAVPILRETVYPFRKALSNLDSPAFNFALPTRAAQFRFDVRLVRDQIENLSNRRVAN
                     FAVVGATRFVIGAAELLFTSTVIQIALALPMAWYFHRATTMALPANALVIPIAGILLP
                     AAVVAVAASYVGHWLAWLPALITRYSLDALTGTIHTIGHLRVSEVRVPTPALLVCCAA
                     AGAFGLALLLARRRMALASVGLAGLLIAAVWIVLFPPKPQWQPGVLEVTAIDVGQGDS
                     LLLITPEGKTLLMDAGGTPGNARSDFDVGEEVVSPYLWSRGIRRLDAAAISHAHGDHL
                     GGMRSVISNFQPRELWYGVESPSRGFRDVEQAARSNHMGLKPFVAGESFEFGRVHVRV
                     LNPQPGWQPRDPPQDDESLVLRLQYGGSSVLLLGDAHKRIEKLLVDEAPQSDLLKVGH
                     HGSATSSSPEFLAAVKPRYAVVSVGFYNSFKLPREDVMERYADAHIPTYRTDLSGAVS
                     FYLDGTNITARPVPR"
     gene            complement(2249..>2506)
                     /locus_tag="VFC15_11760"
     CDS             complement(2249..>2506)
                     /locus_tag="VFC15_11760"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZM10875.1"
                     /translation="DHEELVKVFDTDEESEAMVVRGLLESAGIAAMTQNREAPQDILP
                     GVGGVVVMVRPDQAEEALQTIEEYRRNPTTDADEAVTEEPA"
BASE COUNT          399 a          814 c          758 g          535 t
ORIGIN      
        1 cgcgacagcc cctatggcgg taagcaagag tcggtggatg tcaacgccga gcagcttcag
       61 cttggcgacc gcgtcctcaa cgcagccgtg ggcattcgcc ttacgatcta cagcaaacag
      121 acggaggaag acgaagctcg cgacgaagga accggctcgc cgcttcgcgt gtacacctac
      181 gctcagcgtc tgcatttcat cgccacgctg cggctgccgc gcaactatcg caatcccggc
      241 gcgatggaca tggccggcta tctcgcctcc caaggcattc gcctgaccgg ttcctcccga
      301 gccagcagcg tgcaggttct gccgggtttc gtgggcagcc gcatcgggtt gtggaggagt
      361 gctgcgcgtc gcagcgtact ggcgcacatc atgcagcgct ggcccggcga acgtggcgcg
      421 ctgatgcagg caatgctcat cggagggcgc gccttcttcg ggcgcgacat caagacggaa
      481 ttccagcgca cgggcaccta tcacatcctg gtcgtatccg gcatcaacgt aggcattctc
      541 gccttcacgg tgttctgggc gctgcgcaag ctgcccttcg gcgagacgtg ggccaccatc
      601 cttaccatcc tgctgtcgtg gggatatgcc ttcctcgccg atctcggctc acctatcgtg
      661 cgtgccacca tcacgctcgg gatttacctg gtcacgcgtc tgctgtttcg cgaccgtgcc
      721 gcgctcaacg gactcggcat tgccgccctg ggcatcttgc tggtgaatcc gcgaactctg
      781 ttcgaagcca gcttccagct cacatttctc tccgtgatcg ccgttgctgg gatcgcggtg
      841 cccatcttac gagaaactgt gtacccattt cgcaaggcgc tcagcaacct cgattcgccc
      901 gccttcaatt tcgcgcttcc cacgcgcgcg gcgcaatttc gttttgacgt gcggctggtt
      961 cgcgaccaga tcgaaaacct ctccaaccgg cgagtcgcaa acttcgcagt tgtgggcgcc
     1021 acgcgatttg tcattggcgc ggccgagctc cttttcacct ctaccgttat ccagatcgcg
     1081 ctggcgctgc caatggcgtg gtacttccac cgcgccacta ccatggcgtt gcccgccaac
     1141 gcgctggtga tcccgatcgc ggggattctg ttgcccgccg cagtcgttgc ggttgcagct
     1201 tcctacgtgg gccattggct ggcatggcta ccggcgctca tcacccggta cagcctcgat
     1261 gcgttgacgg gcacgattca tacgattggc catctgcgcg tctccgaagt ccgcgtgccc
     1321 acgccggcgc tattggtctg ctgtgcggct gcgggcgcgt tcggactggc cctgctgctg
     1381 gcgcggcggc ggatggccct ggctagcgtt gggcttgccg gactgctgat tgctgcggtt
     1441 tggattgtgc tatttcctcc aaagccgcaa tggcaacccg gagtgctgga ggttacggcc
     1501 attgatgtgg gacagggcga ttccctgctg ctgatcactc cggagggcaa gactctgctc
     1561 atggatgctg gcggcacgcc cggcaacgcc cgctccgact tcgatgttgg cgaggaggtc
     1621 gtgtcgccat atctctggtc acgcggcatt cgccgactgg atgccgctgc gatctcgcac
     1681 gcccacggcg accatctggg tggtatgcgc agcgtgattt cgaactttca gccgcgtgaa
     1741 ctctggtatg gggtggaatc tccgagtcgc ggcttccggg acgtcgaaca ggcggcgcgc
     1801 tccaatcata tgggtctgaa gccgttcgtc gccggcgaat cgtttgagtt cggaagggtc
     1861 cacgtgcggg tgctgaatcc tcagccggga tggcagccgc gcgatccgcc gcaggatgac
     1921 gagtcgttgg ttctgcgcct gcagtatggc gggagttccg tgctgctgct gggcgacgcg
     1981 cataagcgga tcgagaagtt gctggtggac gaagctccgc aatcggattt gctcaaggtg
     2041 ggacatcacg gcagcgcgac ttccagctcg cccgaattcc tcgccgcggt aaagccgcgt
     2101 tacgccgtgg tttcggtagg cttctacaac tcgttcaaac tcccccggga ggatgtgatg
     2161 gagcgttacg ccgacgccca catcccaacc taccgcaccg acctgtccgg tgccgtctcc
     2221 ttctacctcg acggcacgaa tatcacggct aggccggttc ctcggtgact gcctcgtccg
     2281 catcggtcgt gggattccgg cggtactcct cgatggtctg cagtgcttcc tcggcttgat
     2341 cgggccgaac catcactact acgccaccca cgccgggcag gatgtcctgc ggagcttccc
     2401 ggttctgggt catagcggca atgcccgccg actccagcaa accgcgcacc accatcgcct
     2461 ctgactcttc gtcggtatcg aaaaccttta ccagttcttc gtgatc
//
LOCUS       DASRKK010000513         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_921914, whole genome shotgun sequence.
ACCESSION   DASRKK010000513 DASRKK010000000
VERSION     DASRKK010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921914"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFC15_11765"
     CDS             complement(<1..952)
                     /locus_tag="VFC15_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM10876.1"
                     /translation="MDNEKVVDAAKSTEREYGVGYKLIGKNYETADLYAKVTGTAKYA
                     EDFRADGMLFCKLLLSPMPHARVRRIDAKAALAMPGVKAILTADDLPAPADTLTDNGT
                     VIKASKWGERGLTMEPVYQGEPILAVAAVDELTAAEAIEKIQIDFEPLPFVVDPLDTL
                     RPGGPNPRTDGNVWTRPAASGSQAPGAPMVTELKWTKADFADAEHGRLPMGKAPDEWS
                     YGDLDSGFTNAALVLDETFVTPDTSHQTLETRSALAYWQNGKVYIYTGTQSTAQTLPA
                     IARWLNIDPEKVVFISEYTGGGFGSKITGGVTMIIPALLAK"
     gene            complement(945..1610)
                     /locus_tag="VFC15_11770"
     CDS             complement(945..1610)
                     /locus_tag="VFC15_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM10877.1"
                     /translation="MPEKASSKAIQDVPEEDVLKAVSRRNFIKGVIAGGVAVSSASYL
                     FRASSILQGQQSAAGVGERLITLNVNGQQRRVDVMKQETLAWTLRYKLGLTGTKLGCD
                     RAECGACTVLLDDIPYYSCSVLTHTVRGRKVVTIEGLASADGTLHPVQQGVIDEQGFQ
                     CAFCMSGFIMTTVGYLKTNPNPTRQELAHGVSGNLCRCQDYDKILTALMRGAENMRGT
                     NLG"
     gene            2060..2521
                     /locus_tag="VFC15_11775"
     CDS             2060..2521
                     /locus_tag="VFC15_11775"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HZM10878.1"
                     /translation="MGEYKGTPGELIYEYTVQFAQVVEYGISANAVFSGQMQPPAEGA
                     RFDVYFEGPITGPKMKGMVKGVDYLHIRADGRCQLNIHAEITTEDGKKIALAADGVAI
                     PEEGSLVFQLRENVTLTTNHPEYSWLNPIQVWAPGTVDVSKGEIRVKGYAA"
BASE COUNT          598 a          793 c          839 g          602 t
ORIGIN      
        1 tcttcgccag cagcgcgggg atgatcatcg tcacgccgcc cgtgatcttg ctgccgaacc
       61 cgcctccggt gtattcgctg atgaaaacga ctttctctgg atcgatgttc agccagcgcg
      121 caatggcggg cagcgtttgc gcggtgcttt gagtgccggt gtaaatgtag accttgccgt
      181 tctgccagta agccagtgcg ctgcgggtct caagagtctg atgactggtg tcgggagtga
      241 cgaacgtctc gtcaagaacg agcgccgcat tcgtgaatcc ggaatccaga tcgccataag
      301 accattcgtc aggagccttg cccatcggaa gacggccatg ctctgcatcc gcgaagtcgg
      361 ccttggtcca cttaagctcc gtgaccatcg gtgcacccgg agcttgcgaa ccgctcgcag
      421 caggccgggt ccagacgttg ccatcggttc gcgggttcgg accaccaggt cgcaaggtat
      481 cgagcggatc gaccacgaat ggaagcggtt caaaatcgat ctgaattttc tcgatggctt
      541 ccgcggcagt gagttcgtcg actgcggcca ccgcgagaat cggttcaccc tgataaaccg
      601 gctccatggt gagaccgcgt tcaccccact tgctggcctt gatcaccgtt ccattgtcag
      661 taagggtatc ggccggagcg gggagatcgt ccgctgtgag aatggctttg acgccgggca
      721 tggcgagcgc tgccttcgca tcgatgcgcc ttacgcgcgc atgcggcatt gggctgagca
      781 gcagcttgca gaacagcatg ccgtccgcgc gaaaatcttc agcgtacttt gcggtgcccg
      841 tgacttttgc gtagaggtcg gcggtttcgt agttcttacc gatcagcttg taccccactc
      901 cgtactcgcg ctcggtcgat ttggcggcgt caacgacttt ctcgttatcc aaggttagtc
      961 ccccgcatat tctcagcccc gcgcatcaac gcggtaagga tcttgtcgta atcctgacag
     1021 cggcacagat ttcccgagac cccgtgcgcc agctcctggc gggtgggatt gggattcgtc
     1081 ttcagatatc ccactgtggt cataatgaag ccggacatac agaacgcgca ctgaaatccc
     1141 tgttcatcga tgacgccctg ctgtaccgga tgcaaggttc cgtcggcgct tgccaggcct
     1201 tcgatcgtca caactttccg gccacgcact gtgtgtgtga gcaccgaaca cgaataataa
     1261 ggaatgtcgt ctaaaaggac ggtgcaagct ccgcattcag cacgatcaca tccgagcttg
     1321 gtacccgtaa ggcccagctt gtaacgcagc gtccatgcca acgtctcttg cttcatgaca
     1381 tcgacgcgcc gctgctgacc gttaacgttg agagtaatga ggcgctcgcc cacgccggca
     1441 gccgattgct ggccctgcag aatagaggag gcgcgaaaaa gataactcgc ggaggagacg
     1501 gctacgccgc cggcgataac gcctttgatg aagttgcgcc ttgagacagc tttcagcacg
     1561 tcttcttccg gcacgtcttg aattgccttg gaactcgcct tctctggcat gctcggtcac
     1621 cttttatctg gacagccgct gttccagtct tgcacccaaa aatggacgtg gggccacacg
     1681 tttaagcagt ttgattatca gaaagaacgc ccggattcgg gaattttcga tgcagagagc
     1741 gtaactgcgg cgctcgtcgg cgaggatctc atagacgaga ccgcgccgcc caaaaggcta
     1801 ccatcgccga ctcgaaagtc ggtccgtacg ccagccgata tcgcagaatg acacttccgt
     1861 gtagtacgtc acccaggaat tatcgggttt cttgatcgag ttgaatcaat cagtattggt
     1921 ttcggatagg taagccttat ccgaacataa gggatgttac tctcatcgag aactggtttc
     1981 ttaatgagct gcgtccgccg aaacgtgtag gcgggcgggt tcgcaacgat agacaaggcg
     2041 cacagattgg aggattagga tgggcgagta caaaggaacc ccgggagagc taatctacga
     2101 gtacacggta cagttcgcgc aggtggtcga gtatggcatc tcagcgaatg cggtgttttc
     2161 aggccagatg cagccaccgg cggaaggagc gcgcttcgac gtctacttcg aggggcctat
     2221 caccgggccg aagatgaagg ggatggtcaa gggtgtggat taccttcaca tacgcgccga
     2281 tggccgctgc cagctcaaca ttcacgcaga gatcacgacc gaagacggaa agaagatcgc
     2341 gctagctgcc gacggggtgg cgattccgga ggaagggtca ctggtctttc aactccggga
     2401 gaacgtgacg ttgaccacca accacccgga gtactcgtgg ctgaatccga tccaagtctg
     2461 ggcccccggg acggttgacg tgtcgaaggg agaaatccgg gtcaagggat acgctgcctg
     2521 atagcggcgc ggcgcgcacc ggatgaggtg actgtgggcg ggtggtcggc aatctggaag
     2581 taacgccaag ctctaatctg tcagttcgcg caaaatcgcc attgcgtcca tcggactcag
     2641 ttcaagaaac gccctgttgg cacgacgtga gccaagtatc tgcttttctc gcagccagaa
     2701 ttcctttgga acgacagtct cagacgcatt cagggagcga tggggcggcc ggttgacatg
     2761 tcaggtccgt ggcctatggc gccgaggttc ggctcatacc ttccacaatc gggcgtggat
     2821 cgtaggccac gg
//
LOCUS       DASRKK010000514         6384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1020359, whole genome shotgun sequence.
ACCESSION   DASRKK010000514 DASRKK010000000
VERSION     DASRKK010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020359"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..311)
                     /gene="hfq"
                     /locus_tag="VFC15_11780"
     CDS             complement(9..311)
                     /gene="hfq"
                     /locus_tag="VFC15_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZM10879.1"
                     /translation="MENKPAQNIQDSFLNTARKDKCVITIYLLSGVKLSGRIRSFDKY
                     SVVLDTNNQEQLIFKHAISTVVMSRPPHPAERHLEPRAAETSSPPASAAPAPTPES"
     gene            586..1356
                     /locus_tag="VFC15_11785"
     CDS             586..1356
                     /locus_tag="VFC15_11785"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZM10880.1"
                     /translation="MMGTLVAIVAAPQIDFPGGGQTVLMGLRLVHFVAGIVWVGLLYF
                     FNLVNVPFQRELDAKTKLAVVPNLMPKALWWFRWSSVVTVLAGIAYWMHIVGTDVRNA
                     IAGGEQVSGGAMMGGFFVIWTLAFVIEMGVLMSPAEVLKQGPVLGIIVAVVVLAASYV
                     FLGMNQHGWESNRALAIGIGGGLGWFMMLNVWGIIWRMQKKIIRWTEAAVAGTAMPAE
                     AAKVARLAFLVSRVNFWLSFPMLFFMGAASHYMMFLQQ"
     gene            complement(1360..1662)
                     /locus_tag="VFC15_11790"
     CDS             complement(1360..1662)
                     /locus_tag="VFC15_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10881.1"
                     /translation="MISPILRWLGVLAGLGSAILCWFLGDVLSGPTRKGQLLRLDVAH
                     YELIAFLLVPALVLAIVAVFWVPPRHDGEGPAPEGGRLRLLLVFLFLGAFLIGFVH"
     gene            1801..2052
                     /locus_tag="VFC15_11795"
     CDS             1801..2052
                     /locus_tag="VFC15_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10882.1"
                     /translation="MSSKADWMVVAGFLLAATGVVLRVFMMMRSSDAHPAQGAAKEGR
                     ELLRSYGTTFPKSRIPLLMMVMLSVGLVLLLAGLLLELR"
     gene            complement(2061..2675)
                     /locus_tag="VFC15_11800"
     CDS             complement(2061..2675)
                     /locus_tag="VFC15_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10883.1"
                     /translation="MRHHHREARSVPVLRTMTITGVSGSKILLDPTVHEMKLLFAWIA
                     VMAVGIAGWCQAPPGSATASPQKPKVSPLAEYAGEWTATFDGKVWLRLHLELRGDQIT
                     GALLHARNLAVNDNGELKSVSEEQSGETVTSAVLNPDGLLLTFKDQDSQQTDRYTMRL
                     VTAEKDAADLKMIAMSMPPGMPKPKPWRLVKSGIAPANKTPVSH"
     gene            complement(2717..3280)
                     /locus_tag="VFC15_11805"
     CDS             complement(2717..3280)
                     /locus_tag="VFC15_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10884.1"
                     /translation="MFATFPIILFIIGAILLVALPFGISLQSYFRNRGRRTVVCPDNH
                     QPVTVELDSKFALATALRGEEHTRLHSCSRWPEKGDCDQECLAQVDPTPENLERLLYK
                     WYQGKTCAICDRSLTPADWRRSRLALLNENHKLFELHHMHLDEVPSALDHMKPLCWNC
                     HQEERVRQASPPRILKGDRRNLGSQSS"
     gene            3399..5717
                     /locus_tag="VFC15_11810"
     CDS             3399..5717
                     /locus_tag="VFC15_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZM10885.1"
                     /translation="MRSATWLGWVQLFIIGGTSKPTSSVITLSIQLAARVATSHKLRL
                     PPALYSCVAFALMVFLTGMAAPSFAEPASGGKPRITLDEFFNSVEFSKVALSPNGHMV
                     AIATEWADWEGERFREDLWLWRDSDGVLIPLTQSGHDSDPMWSPDGKWIAFLSDRDSD
                     SDSDKSDKADDDQKGVTHVYLIPVGGGEAFPVTRGAEDVHSFAWSPDSKTLYFATRIP
                     WTKKQKDEYKEQWKDVVRYREQERGDVISRIGVADALSRQIAMGTEEPKKSKKDKKKK
                     DARDKDEDEETGETPGAQAVVTSQYRVKSMAISPDGSQLAYLTDSASQRFEGLKEIEI
                     YVADTSKAGPQTTARQLTKNEALEDELQWSRDGKNLFFEVVQGSVEGAYADLQPRVYS
                     VNVASGQAIRWAASFEGAVMHYALGDNGSLLAPGMLGTATALYQQATPQEAFRKLAGW
                     PGTYGRAATATNSPRIAFTYSEVDKPTEVYIADGADTLSAARPITSFNKLFTERELPK
                     GKPFRWTSDDGTPVEGMLLYPPGKFGAKNLRLLVLIHGGPMDADGNYFGADWYDWGIY
                     AASQGWLVFRPNYRGSAGYGDKFAMAIVPKIVSAPGRDVLSGVEALVKAGIANPMQMT
                     IGGYSYGGYLTNWLITQTNEFRAAVTGAGAVEHISNWGNDDTAYDDAFFLGGMAWDPK
                     ARQNYLDEAAIFQVDKIKTPTHIVAGGSDVRVAVEEDYLLEHALHMLGIPSSLLIFPD
                     EGHELDQNPWHGKIKVREEAEWIEKYCACPVK"
     gene            5820..>6384
                     /locus_tag="VFC15_11815"
     CDS             5820..>6384
                     /locus_tag="VFC15_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM10886.1"
                     /translation="MKKKTSKTAGKKTGARRAAKLAPVKPSKTARKTAAAKPAKASPK
                     ETLAGKKGAAAAVSATAPANDGKLTFNHAMIYCKDVERGVGFYRDLMGFKLIEDFRYE
                     GKSVYARLRAPGGDGTIALHQAGPGASLASEGVRLYFEVCDLDEFCRKLMQKGFYITQ
                     MPRMMPWGWRHAYLNDPDGHEISLYWAG"
BASE COUNT         1373 a         1673 c         2121 g         1217 t
ORIGIN      
        1 gaaatatgct aactctccgg ggtgggcgca ggggccgcgg atgccggggg agaggaggtc
       61 tcagcggccc tcggctccaa gtgacgctcg gcgggatgtg gcggacgcga catgaccacc
      121 gtggaaatcg cgtgcttgaa aatcaactgc tcctgattgt tggtgtctaa gaccaccgag
      181 tacttgtcaa aggagcgaat gcggcctgat agtttcactc cgctcaaaag ataaatggtg
      241 atgacacatt tgtccttgcg ggccgtatta agaaaggaat cctgtatgtt ctgtgctggc
      301 ttgttttcca tgggtggtcc tccttgcaac cggcaaaaac agctcagccc cagagaccgt
      361 ggccccaaaa cgtttccggg tctgtagaca acagtcctcc acgcacgcgg tagaacattg
      421 ccgggtgcgg gagagtaaaa tccagcagct taacgataag tctcctctgg cacaatttca
      481 actaaaacca gcaaattggt gagtgatgta gaattcgccc gaccgcaacg gggcggaaaa
      541 aaccgctacc tgggcggcgg cctggtccaa ttaaggggag aaaacatgat gggaacactg
      601 gttgcaatcg tcgcggcgcc acagatcgac tttcccggag gcggccaaac cgtgctgatg
      661 gggctgcgct tggtgcattt tgtggccggg atcgtatggg tcggattgct ctactttttc
      721 aacctggtga atgttccctt tcagcgcgag ctggacgcga aaaccaagct ggcggtggtg
      781 cccaacctga tgccgaaagc gctgtggtgg ttccgctgga gctcggtggt gacggtgctg
      841 gcgggcatcg cgtactggat gcatatcgtc gggaccgacg tgcgaaacgc aattgcaggc
      901 ggagagcagg tgtcgggcgg cgcgatgatg ggcggattct tcgtgatctg gacgctggcg
      961 ttcgtgatcg agatgggagt gctgatgtcg ccggcggagg tcttaaaaca gggaccggta
     1021 ctgggaatca ttgtggcggt agtggtgctg gcggcgagct acgtgttcct ggggatgaac
     1081 cagcacggat gggagagcaa ccgcgcgctg gccatcggca tcggcggcgg gctgggctgg
     1141 ttcatgatgc tgaatgtgtg gggcatcatc tggcggatgc agaagaagat catccggtgg
     1201 acggaagcgg cggtggctgg cacggccatg ccggcggaag cggccaaggt ggcgcgactg
     1261 gcgttcctgg tgtcgcgagt gaacttctgg ctgtcgtttc ccatgttgtt cttcatggga
     1321 gcagcgagcc attacatgat gttcttgcag cagtgagggt tagtgcacga atccgatgag
     1381 aaacgcaccg aggaagagga agaccaaaag aaggcggagg cggccgcctt cgggtgcggg
     1441 accctcgccg tcgtgccgcg ggggcaccca gaagacggcg acgatggcga gaacgagcgc
     1501 gggcacgagg agaaaggcga tcagctcata gtgagcgacg tcgagccgca gcagttggcc
     1561 ttttcgagtg ggaccggaaa gcacgtcgcc caaaaaccag caaagtattg cgctaccgag
     1621 tccggccaac actccgagcc agcgcaatat gggagaaatc attcgactcc ttgggtgcga
     1681 aagttagaag tgtaacaggg agcgcgcgtc cttaagaaat gacggacgtg acaaatctct
     1741 taacacggtg ttgctttaaa ggtaaactgc tggcgcggca cttccgggag caatacgatc
     1801 atgagttcca aggcagattg gatggtggtt gcaggatttt tattggctgc aacgggagtg
     1861 gtgctgcgcg tgttcatgat gatgcgatcg agcgacgcgc atccggcaca gggggcggcc
     1921 aaggaagggc gagaactgct tcgctcctac ggcaccacat ttcccaagag ccgaattcca
     1981 ttgttgatga tggtaatgct cagcgtgggg ctggttctgc tgctcgcagg gttgctgctg
     2041 gaattgcgat aaactccgtt tcagtgggag acaggcgttt tattggcagg ggcgatgccg
     2101 gacttcacca gcctccacgg cttgggcttg ggcatcccgg gaggcatgga catggcaatc
     2161 atcttcaggt cagccgcgtc tttttctgcg gtcacgagcc gcatcgtata acgatctgtt
     2221 tgctgtgaat cctggtcctt gaaagtcagc agcagtccgt caggattgag caccgcagag
     2281 gtgacagtct cgcccgattg ctcttcgctg accgacttta actctccgtt gtcattcacc
     2341 gcgaggttgc gcgcatgcag taacgcgccg gtgatctggt cgccgcgcag ttcaaggtgc
     2401 aatcgcaacc ataccttgcc atcgaaagtc gctgtccatt cgcccgcgta ttccgcgagc
     2461 ggcgagactt tcggcttttg cggggacgct gtggcggagc ccggcggagc ttgacaccag
     2521 ccggcgatgc ccacggccat gacggctatc catgcgaaaa gcaacttcat ttcatgcacc
     2581 gttggatcaa gcagaatttt actccccgac acgccagtga tcgtcatggt acgaagaacg
     2641 ggaacgctgc gcgcttcccg gtgatgatgg cgcatggtgc aacaaccgag gtccgcgacc
     2701 tgagaggaag tagtgactaa gaactctgtg agccgagatt gcgacgatcg ccctttagta
     2761 tgcggggcgg agatgcctgg cgaacacgct cctcctggtg acagttccag cagagaggct
     2821 tcatgtgatc cagcgccgaa ggcacttcgt cgaggtgcat gtgatgcagt tcgaagagct
     2881 tgtggttctc gttgagcaga gcgaggcggc tgcgcctcca gtcggcgggt gtcaacgaac
     2941 gatcgcaaat ggcacaggtt ttgccctggt accacttgta taacaggcgc tcgaggttct
     3001 cgggagtcgg gtcgacctgc gccaggcact cctgatcgca gtcacctttc tcgggccaac
     3061 gcgagcagga atgcaggcgg gtgtgttcct cgcctcgcag cgcggtggca agggcaaact
     3121 tgctgtcgag ttcgacagta acgggctgat gattatcggg acagacgacg gtgcggcggc
     3181 cccggttgcg aaagtaggac tgcaagctga taccaaacgg aagcgccacg agcagaatcg
     3241 cgccgatgat gaaaagaatt atgggaaatg tcgcgaacat ggaagcacct cccgccaaaa
     3301 acaacgggcc tgacagcttc tttgtagcgt ggccggcacc aaaacgcagt gattgacaac
     3361 acgagcgcgt gtgacgcggt ggcggagcgg aaggaccggt gcggtcggcg acttggttgg
     3421 gctgggtaca gctgtttata atcggcggca ccagcaaacc aacttcatcg gtgatcacgt
     3481 tgtctatcca gttggcggca cgcgttgcga catcgcataa attgcgtctc cctccggctc
     3541 tctattcttg cgtggccttc gcactcatgg ttttcctgac gggcatggcg gctccatcct
     3601 tcgcggagcc cgcgtccggc ggcaagccgc gaatcacgct cgacgagttc ttcaactcgg
     3661 tggaattcag caaggtggca ctctcgccca atggacacat ggtagccatc gccaccgagt
     3721 gggcggattg ggaaggtgag cgctttcgcg aagacttatg gttgtggcgg gacagcgatg
     3781 gcgtgctgat tccgctgacg cagtcggggc acgattcgga cccgatgtgg tctcccgatg
     3841 ggaagtggat tgcgtttctg tcggaccgcg acagcgatag cgacagcgac aaatccgaca
     3901 aggcggacga cgaccagaag ggggtaacgc acgtgtacct cattccggtg ggcggcggcg
     3961 aggcgtttcc tgtgacacgc ggcgctgagg atgtgcactc gttcgcgtgg agtccagatt
     4021 cgaagacgct gtacttcgca acacgcattc cgtggacgaa gaaacagaag gacgaataca
     4081 aagagcagtg gaaagacgtg gtccgctatc gcgagcagga acgtggagac gtgatctcgc
     4141 gaattggcgt ggcggacgcg ctgtcgcggc agatcgcgat gggcacggaa gaacccaaga
     4201 agagcaagaa ggacaagaag aagaaagatg ccagagataa agatgaggac gaggagacgg
     4261 gcgagactcc gggcgcgcag gcagtagtga cgtcgcagta tcgcgtgaag agcatggcga
     4321 tttcgcccga tggcagccag ctcgcgtacc tgaccgatag cgcttcgcag cgcttcgagg
     4381 gcctgaagga gatcgagatc tatgttgcgg acacgagcaa ggcgggtccg cagactacgg
     4441 cgcggcaatt gacgaagaac gaggcgctcg aagacgaact gcagtggtcg cgcgatggaa
     4501 agaatctgtt tttcgaggtc gttcaaggtt cggtggaagg cgcgtacgcc gacttacagc
     4561 cgcgcgttta ctcggtcaat gtcgccagcg gacaagcaat ccgctgggcg gcgagctttg
     4621 agggcgcggt gatgcactac gcgctggggg acaatgggtc gctgctggct cccggaatgt
     4681 tgggcacggc gaccgcgctc tatcagcagg caacgccgca ggaggccttc cgcaaactcg
     4741 cgggatggcc cggcacctat gggcgcgcgg cgaccgcgac gaattcgcca cgtatcgcgt
     4801 tcacttactc cgaagtggac aagcccaccg aggtttacat cgccgacggc gcggacacgc
     4861 tcagcgcggc gcggccgatc acgtcgttca acaagttgtt caccgagcgc gagcttccca
     4921 agggcaagcc gtttcgctgg acgtccgatg atggcacgcc ggtggaaggc atgctgctct
     4981 atcctcccgg aaaattcgga gcgaagaact tgcggctgtt ggtgctcata cacggcggac
     5041 cgatggacgc cgacggaaac tactttggcg ctgactggta cgactgggga atctacgctg
     5101 cgagccaggg atggctggtg tttcgtccca actatcgcgg gtcggcgggc tatggcgaca
     5161 agttcgcgat ggcgattgtg ccgaagatcg tgtcggctcc gggccgcgac gtcctgagtg
     5221 gcgtggaggc gctggtgaag gcaggaattg ccaatcccat gcagatgacg atcggcggct
     5281 atagctatgg cggctacctg accaactggc tgatcacgca gaccaatgag ttcagggcgg
     5341 cggtgaccgg cgcgggtgct gtggaacaca tcagcaactg gggcaacgac gatacagcct
     5401 acgacgacgc gttcttcctg ggcggaatgg cgtgggaccc gaaggcgcgg cagaattacc
     5461 tcgatgaggc cgcgatcttc caggtcgaca agatcaagac accgacgcac atcgtggcgg
     5521 gcggaagcga cgtccgggtt gccgtcgagg aagattacct gctggagcac gcgctgcaca
     5581 tgctgggcat tccgagttcg ttgctgatct tcccagatga agggcatgag ttggaccaga
     5641 atccgtggca cggcaagatc aaagttcgcg aggaggcgga gtggatcgag aagtactgcg
     5701 cgtgcccggt gaagtaaggg cggcggctcg ctgtcggttt gtcctaaagt tgcttgacaa
     5761 tcgcgtcgcg tctgaacata cttgcgccgt ttgatgcgcg gaaagcacga cggtgatgca
     5821 tgaagaagaa aacaagtaaa acggcaggca agaagaccgg agcgcgacgt gcggcgaagc
     5881 tcgcgccggt gaagccatcg aagaccgcga ggaaaactgc ggctgcgaag cctgcgaaag
     5941 cgtctccgaa agaaactttg gctgggaaaa agggtgcggc ggcagcagtg tccgcaactg
     6001 cgcctgcgaa cgatggcaag ctcaccttca atcacgccat gatttactgc aaggacgtgg
     6061 agcgcggcgt gggcttctac cgcgacctga tgggattcaa actgattgag gactttcgct
     6121 acgaaggcaa gtccgtatat gcgaggctgc gcgctccggg cggcgacggg acgatcgccc
     6181 tgcatcaagc gggcccgggc gcgtcgctgg ccagcgaagg cgtgcggctg tacttcgaag
     6241 tgtgcgatct cgacgagttc tgtcgcaagc ttatgcaaaa aggcttctac atcacgcaga
     6301 tgccgcgcat gatgccctgg ggctggcgac atgcctacct caacgatcct gacggacacg
     6361 agatcagcct gtattgggca ggcg
//
LOCUS       DASRKK010000515         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_336219, whole genome shotgun sequence.
ACCESSION   DASRKK010000515 DASRKK010000000
VERSION     DASRKK010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336219"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFC15_11820"
     CDS             <1..579
                     /locus_tag="VFC15_11820"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HZM10887.1"
                     /translation="QLKKSTQVFLDSPMAISATEIFRRHPECLEPATAKLFQEGHDPF
                     ALPGLHFTRETAESIAINNIHGGAIIMAGAGMCTGGRVRHHLQHNLGRADSSIVFVGY
                     AAIGTLARRIIDGEKQVNIFGENIPVRARIYTINGFSAHADQAELLAWQKKTSAKRTF
                     LVHGEEDAMKQFAAHLADTRVDMPGPNQVFEL"
     gene            complement(812..1393)
                     /locus_tag="VFC15_11825"
     CDS             complement(812..1393)
                     /locus_tag="VFC15_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10888.1"
                     /translation="MIAHLGNGASLTAVKDGQSIDTSMGLTPTGGVIMGTRCGDLDPG
                     VLIYLVREKNFDAARLEALVDHHSGLLGISGIDSDMRRLHELSPSNADARLAIEMFCY
                     SIRKQVAAMIVALDAVDLLVFTGGIGENDAKVRAEICGGLSWLGISLDTTRNRSLEDP
                     ISNPTSRCSVRVLPSEEDAQVATHTWELASGIS"
     gene            complement(1393..2158)
                     /locus_tag="VFC15_11830"
                     /pseudo
     CDS             complement(1393..2158)
                     /locus_tag="VFC15_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2700..>3472
                     /locus_tag="VFC15_11835"
     CDS             2700..>3472
                     /locus_tag="VFC15_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10889.1"
                     /translation="MTSQPDAREGQAGPLGVEEGPVRPTKPGNAGGGKGPWFKATQDA
                     AKTRRLEMILAPPESVQKLQTALRAKAKGAPNYRFYLLYDKLYRKDVLTYAYQRCKAN
                     KGAPGIDGQEFADIEAYGEERWLGELADILRRKTYRAEAVRRVWIPKAGGSNKLRPLG
                     IPRIADRVVMTAATVVLESIFEVDMPAEQHGYRPNLSAHTAVRSVDRLINGGHSLTDS
                     DARSMATSGRGSGVVGYNVQIAVKAGHHLIVTHEVTNVGT"
BASE COUNT          779 a          962 c         1033 g          698 t
ORIGIN      
        1 cagctcaaga aatcgacaca agtgtttctg gattcaccga tggctatatc agccacggag
       61 atctttcgac gccatccgga gtgccttgag ccagcgaccg ccaagttgtt tcaagaagga
      121 cacgaccctt ttgccttacc aggattgcat ttcacgcggg agaccgccga atccattgcg
      181 ataaacaaca tccatggcgg ggccattatt atggctggcg ctggcatgtg caccggcggt
      241 cgcgtgcgcc atcacctcca gcacaatctc ggtcgtgcgg attcgagcat cgtgttcgtc
      301 ggctatgcag ccattgggac tctcgcgcgc cgtattatcg atggtgagaa acaggtgaac
      361 atttttggtg agaatattcc ggttcgggct cgaatctaca ccattaatgg tttctccgcc
      421 catgccgacc aggcggagct gcttgcttgg caaaagaaaa ctagcgcgaa acgtacgttc
      481 cttgttcacg gtgaagaaga cgccatgaag cagtttgcgg cacatcttgc cgacactcgc
      541 gtcgatatgc cagggccaaa tcaagttttt gagctttgag ctggcgcctt gacgatactc
      601 gtacaacgcc gagttttgct tgagcaaaaa agccatccga cctgccaatc ccaccgctcc
      661 gagagacctc agttagttcc agcgacaagg cgatcttgaa gatgacgagg atttttgtag
      721 ccatcgattc cagaaattat gtcgtcttga actggaggct tataaatctt atggcttaca
      781 ccccaccagg gcgttgatga cccgcgtcga atcacgaaat accagaggct agttcccagg
      841 tatgagtggc gacctgcgcg tcttcttccg aggggagcac tcggaccgaa caacgcgacg
      901 ttggattgct gatggggtct tccaaagatc gatttcgtgt tgtatcgagg ctgataccaa
      961 gccacgagag accgccgcag atctcggccc tcacctttgc atcgttttct ccgatcccac
     1021 cggtaaagac cagcagatcc accgcatcga gcgcaacgat catggccgcc acctgtttgc
     1081 ggattgagta acagaacatt tcgattgcca gccgtgcatc tgcgttggat ggagataatt
     1141 catgcagccg acgcatgtcg ctatcgattc cggaaattcc gagtaggccg gagtggtgat
     1201 cgaccaacgc ttccagacgc gcggcgtcga agttcttttc gcgcaccaaa tagatcagca
     1261 caccgggatc gagatcgccg cagcgggtcc ccatgatcac cccacccgtc ggggtcagac
     1321 ccatgctggt gtcaatggat tggccatctt taacggcggt gagactggcg ccattgccga
     1381 gatgcgcaat tattagggcg cggactcata agcacgtagc caaatccgta tagatgcaag
     1441 cttgatgaag gcaagataat tggctgcgag cctgtcgtac cgggtcgcga tgcgccgaca
     1501 ctgcttgatt ttgttgaaaa atcgctcaac caagtttcgc gcacggtaga gatatgggct
     1561 gaagcagatt gcttccttac gatttcgttt cggcgggatg tttgcccacg caccttgctc
     1621 actggcaagt tccctgatcc agtccgcgtc atatccccga tccgccaaca acattgtccg
     1681 tggccttaat tcgttcagga gagccgaaca cagtcgattg tcgtgcgcct caccgggcgt
     1741 aaggccgagt tggactggta ggccatttgt gtccacaacg gcgtgattct tggttgtttg
     1801 ttagtccacc tcgcgaccgc cccatatgct gctcactatt gcccgcgatg caggctccgt
     1861 gctgatgcac acgcacgaca gacgtatcga tcatttgcac cgccgcatca tgagtttctg
     1921 ccagtgcgtc cattatctgg tcccagacgc cagccctccc ccagcgaacg aagcgattgt
     1981 aacaggtcgt ataggggcca aatgtctccg gcagatcgcg ccatggcgcg cctgatcgca
     2041 agacccaaaa gattccattg aggacgcgcc ggtcgttcac acgcggcacg ccacgtggct
     2101 tattcgggag gaatggcctg agggcggccc attcatagtc cgagagttca taccgcatga
     2161 ttcgaggctc cagtttggga gcttgaatca cggctagtgc gatgggatca acgatgaaac
     2221 gggatgccca gcgttgatgg cgcaccgtta cgtttgtcgc ggtgcaccat tttgtcgcct
     2281 cttggacaac aacggacaaa ggtcggtgcg gaggctgaat aattacgcag cgattgaccc
     2341 aactgcgacg tgcgccgtgg aaaggcgcga tttcccagtg ggagacaaac ccacccggca
     2401 actttcgctc cagccggtag caatcggagc agtcgtggag gtgacgaagc ggctgaagcc
     2461 ttcggtgcag agggtcactt tggtgactcg gcgagcatgc gggttgcaac gtgtagtgaa
     2521 cgctgagcag gctccgaaaa gggtgatgtg gaagccgacc cgaacgtcgc aacggggaag
     2581 gctgttgtcg ctggggaagt gagcgaacat gcacccagcg gttccaccgg ggtattggcg
     2641 gcggcacgca tgcacaggga aatcgacgca acacgggaag ccccgagagg tggctgggcg
     2701 tgaccagcca accggacgcc cgcgagggac aggccgggcc actcggggtg gaggaggggc
     2761 ccgtacgacc gacgaagccg ggtaatgccg gtggaggaaa ggggccctgg ttcaaggcaa
     2821 cgcaagacgc ggcgaagacc aggagattgg agatgatcct cgcacctccg gaaagtgttc
     2881 agaagctgca gacggcgtta cgggcgaaag ctaagggagc gccgaactat cggttctatc
     2941 tgctgtacga caagctgtac cgcaaggacg tgctgaccta cgcgtatcaa cggtgcaaag
     3001 ccaacaaagg cgcaccgggg atcgacggtc aggaatttgc ggacatcgag gcgtacggcg
     3061 aagagcggtg gctcggcgaa ctggcggaca tactccgcag gaagacgtat cgagccgagg
     3121 cagttcgacg ggtgtggatt ccaaaagcgg gcggcagcaa taaactacgg ccgctgggga
     3181 ttcctcgaat tgcagaccgc gtggtgatga cggcggcgac ggtggtgcta gaatccatct
     3241 tcgaggtcga catgccggcg gagcaacatg gctacagacc aaatctcagc gcccacacgg
     3301 cggtgcgttc tgtcgaccgc ctaatcaacg ggggacactc gctgaccgat tccgatgccc
     3361 gctcgatggc gaccagcggc cgcggctccg gcgtcgtggg ctacaacgtc cagattgccg
     3421 tgaaggccgg gcatcatctg atcgtcacgc atgaggtgac caacgtcggg ac
//
LOCUS       DASRKK010000516         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1411814, whole genome shotgun sequence.
ACCESSION   DASRKK010000516 DASRKK010000000
VERSION     DASRKK010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411814"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFC15_11840"
     CDS             complement(<1..878)
                     /locus_tag="VFC15_11840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HZM10890.1"
                     /translation="MIERAESVVRDNPFLELRLDYLSQPAAALPKLKSFLEMHPEATV
                     IATCRRAVNGGKFKGSVAAELGILLKAADAGFPLVDIELQTAKSMKADQLRGLTDRVG
                     LIISFHDFRATKKLEEQFAEMGKYPADFYKVVSTATNLYDNVVMMKFLEANSAKHEMV
                     ALCMGEQGIISRVLGVRAGSIFTFGAAKSGEETAPGQVPFNELRDIYRIDNADAATQV
                     YGVAGDPVEHSLSPIMMNAAFRREAVNAVYLSLHAKSLKDLMACVRDIPIRGLSITMP
                     YKQAIVDALENSDLLTR"
     gene            1118..1669
                     /locus_tag="VFC15_11845"
     CDS             1118..1669
                     /locus_tag="VFC15_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10891.1"
                     /translation="MLMRLELPHRYEVARASEAPAGISQPPVRYRPAHSYDVGSDVLL
                     LEFRSPSSAPWIGSFACRTSYSSGLSMVISSPDPMTAFVIAGGEGYSVQVDSPGHWQP
                     LPVSPVVFAEVLPEQGLVVLGSGNALAVYDPKGLVWFRRVVENELKLRSISEDLIHFS
                     GLDTEAGKTMCLSAALRSGKEIE"
     gene            complement(1681..2265)
                     /gene="amrA"
                     /locus_tag="VFC15_11850"
     CDS             complement(1681..2265)
                     /gene="amrA"
                     /locus_tag="VFC15_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZM10892.1"
                     /translation="MSPYSEEPRTATAVQPCEYSPEERELLLRLAHRSIELALEGRGI
                     DLTPPTAHLAESRGAFTTLHLEGELRGCIGFVLPIQSLYAAVAESARAAAFDDPRFQP
                     VTPAEAQHLKIEISVLSPLQPIRPEEVVVGQHGLVATQGNRRGLLLPQVPVEWKWDRE
                     TFLAQTCLKAGLPADAWQHGAQLQAFTAEVFGES"
BASE COUNT          462 a          694 c          688 g          485 t
ORIGIN      
        1 cgggtcagca ggtcggaatt ctccagcgca tccacgatag cctgcttgta cggcatggtg
       61 atgctgagac cgcgaatggg tatgtcgcga acgcaggcca tcagatcttt caggctcttg
      121 gcgtgcagcg acaaatagac ggcgttcacg gcttcgcggc gaaacgccgc gttcatcatg
      181 atgggtgaga gcgaatgctc aaccgggtca cccgccaccc cgtaaacctg ggttgccgca
      241 tccgcgttat cgatgcggta gatgtccctc agttcattga acggtacctg tccgggagcg
      301 gtctcttcgc cactcttcgc cgctccgaag gtaaagatgc tgcccgcccg aactcccagc
      361 acacggctga tgatcccctg ttcccccatg cacaaggcca ccatctcgtg cttggccgaa
      421 ttcgcctcca ggaacttcat catcactacg ttgtcgtaga gattggtcgc cgtcgagacc
      481 accttgtaga aatcggcggg atacttgccc atctcggcga actgctcttc cagcttcttg
      541 gtggcacgaa agtcgtggaa ggagatgatc aagccgaccc ggtcggtaag gccgcgcagt
      601 tggtcggctt tcatactctt ggcggtctgt aactcgatat ccaccaaggg aaagccagcg
      661 tcggccgcct tcagcaggat ccccaattca gcggcgaccg agcccttgaa tttgccccca
      721 ttgaccgccc gccggcaagt ggcgattacc gtcgcctccg ggtgcatctc caggaaactc
      781 ttcagcttgg gaagggctgc tgccggctgt gaaaggtagt ctaaccttaa ctccagaaat
      841 gggttatcgc gcactaccga ctcggcgcgt tcgatcatgg tgggggcatc ggagcccacc
      901 accggcacac aaacccgggg cagccggaag cgatggatac gagatgcgta actgcttgca
      961 gacgcgcgca tgtagctgcc cgatgttaca gatgcacaag aatagttgca accatcttct
     1021 taagaaatgt tgcaaagaca ggcagaaggg tctatcggca ggtacgaatc aggcgtgcta
     1081 ctagcagcgt atactcttgc tcaggaagga ctttgttatg ttgatgcgcc tggaattacc
     1141 gcaccgttac gaggttgcgc gcgcgagcga agctcccgcc ggcatcagtc agccacccgt
     1201 tcgataccgt cctgcccatt cctatgatgt cggcagcgac gtcctactgc ttgagttccg
     1261 gtcgccaagc tctgcgcctt ggatcggcag tttcgcctgc cgaaccagct acagcagcgg
     1321 ccttagcatg gtgatcagtt ctcccgaccc aatgaccgca ttcgtgatcg ccggcggcga
     1381 ggggtattca gtccaagtcg attctcccgg gcattggcaa ccgctgccgg tttcacccgt
     1441 ggtgtttgcc gaagtgctgc cggaacaggg tctcgtggtt ctaggcagcg gcaatgccct
     1501 tgctgtgtac gatcccaaag gcttggtttg gttccggcgc gtggtggaaa acgaattgaa
     1561 gctgcggtcg atctccgaag atctcatcca tttcagcgga ctggacaccg aagcgggcaa
     1621 gacgatgtgc ttgagtgccg ctctccgcag cggtaaggaa atcgaataga aggttggccg
     1681 ctaggattcg ccaaatacct ctgcggtgaa cgcttgtaat tgggcgccat gctgccaggc
     1741 atccgccggt agtccggcct tcaggcaggt ctgtgccaga aaggtttcgc ggtcccattt
     1801 ccactcgacg ggaacctgcg gcaacagcaa tccacggcga ttgccctgag tcgcgacaag
     1861 tccgtgttgg ccaaccacga cctcttcggg tcgaatgggc tgcaaaggcg agagcacgct
     1921 gatctcaatc ttcaggtgtt gtgcctccgc tggcgtcacc ggctggaaac gcgggtcgtc
     1981 gaaggctgcg gccctggctg actccgcaac cgctgcgtag agcgactgga tgggcaacac
     2041 gaatccgatg catccgcgca gttcaccctc cagatgcaaa gtggtgaagg ccccgcgtga
     2101 ctcggccagg tgggccgttg gcggagttag atcgataccg cgtccttcca gggccaattc
     2161 aatcgagcga tgagcgagcc gcagaagcag ttctcgttct tccggcgaat actcgcacgg
     2221 ttgcactgcg gtcgcggtgc gtggctcttc agagtacggg gacatcgttg cgcttcctgc
     2281 gccggctcag cacaaacgaa cgccgcttgc ggcgcttctc ggcatggcg
//
LOCUS       DASRKK010000517        12967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1215994, whole genome shotgun sequence.
ACCESSION   DASRKK010000517 DASRKK010000000
VERSION     DASRKK010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215994"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VFC15_11855"
     CDS             <1..160
                     /locus_tag="VFC15_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HZM10893.1"
                     /translation="SLIGSAKLNGLDPEAYLREVLTRIADHPITHIEELLPWQIAASL
                     PPAIEPAA"
     gene            complement(315..2882)
                     /locus_tag="VFC15_11860"
     CDS             complement(315..2882)
                     /locus_tag="VFC15_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM10894.1"
                     /translation="MIRSMELNGELDRWVYKRCMLRRSMLLTSFCGLLAFISPLVFAQ
                     QKPNVAGDYVGSLGPLHLKLHLKIDTEGALNGTLDSPDQGASGIPCADFHFDGQSLSF
                     AVPAVNGTWKGTVGSQGALLTGIWNQGTPVPLNFSRDTFVFAAKPSAVDGIWLGTLRD
                     GSKSLRIQLIVKGDITGEEFCSNDSLDQRAMGLECGNVLLEGNKFSFDIPVVSGHWGG
                     TLSADGKTLTGTWNQGHPLPLNFEKQTVAISLPPVHPPTYDTAMAPVPAAQLQAVLSK
                     DLSQALTSGALAPSTGAGVTIGIVDHGERQVFSYGTAKPDSIFEIGSITKTFTGLLLA
                     QIVAQGKVKFDEPVRELLPAGTVAKPTGNEITLLDLATQHSGLPRMPDNFNPPDGSNP
                     YADYRAADLYAFLTKHGVAKPADATFLYSNLGFALLGQALAVRAGTTYPQLLQEEITG
                     PLGMKDTIVALSAEQQLRLIAGYDGAHHPAHAWDLVAFAGAGAIRSTADDMLTYLQAN
                     LHPDKLPSGATKTPNGETLPAALAQSHVLRADAMAGMRIALAWLYAPVTGGYWHDGET
                     AGYSSLAAFNPKADHAVVVLVNTAGDFAHRLGEHISERLAGKPAISLGTANDKDAYDR
                     AIQNYDEAIRLDPRSADAFAGRGSAYLLKGDYDSAILDYNEAIRLNPKSASAFNGRGA
                     SYFAKGDYDRAIQDYNEAIRLDPKTPRALLNRGLVNLYSGHFSDAQQDFSQNLRLDRT
                     DLYGVIWLYLSRVKAGADGKDELKMNTAGLDLSKWPGPLIQLYLGQSTLDDTFRATGG
                     DSDQKCEYSFYVGEYRALRGEHPEALALFRSASDGCPKDFIEYVPAVIELNNLEKQK"
     gene            3510..3884
                     /gene="tnpB"
                     /locus_tag="VFC15_11865"
     CDS             3510..3884
                     /gene="tnpB"
                     /locus_tag="VFC15_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZM10895.1"
                     /translation="MFGLGPATRIYLAAGVTDMRKGFEGLYGLVRDRLSCEPLSGHLF
                     LFCNAQRNRLKVLVWDGSGLWVCAKRLEKGRFTWPQSGDAQGKVVLTQEELSLLLGGI
                     DLCKTKHKRWYRKAPPEGHAAA"
     gene            3930..5450
                     /locus_tag="VFC15_11870"
     CDS             3930..5450
                     /locus_tag="VFC15_11870"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM10896.1"
                     /translation="MSEAPIPSAPADLELIAQLKSRLQYAELRIRVLEERLRLMRIEK
                     YGAGGEKLSQAQMELFELSPVVSKMIEQAESECTPVRRPTHRSCKHPGRQGLPANLPR
                     VERILPCTPDQRVCKRCGKETVVIGYEESSQLDVEPAKYFVLVTKREKRACRSCEELG
                     VVSAPMPPRIIEKCLASDRIVIDTVISKYCNHTPLYRQSMILGRDIGLEISRATLDGW
                     VLKVGELLIPMVAAMRRELISGSYIQADETPVDVQMREGRGKNHQAYLWQYGRPGGSV
                     VFDFRLGRGRDGPRRFLGQFEGILQTDGYTAYEQIGGTRMVHAACWAHARRQFFEAVQ
                     LNPQDPVATPIVARMDELFAVDAEARRRGIGLTGRHALRRERAKPLLDDIRSKIEAAQ
                     SAALPSSALSKACQYALTLWRKLTRFLEYPELELSNNLAENSMRPVALGRKNWIHIGS
                     PQAGPKIAAILSVVESCRRLKLPVRDYLAAVLPGFADLPIQRLPDLTPAAWVVQHS"
     gene            complement(5591..8809)
                     /locus_tag="VFC15_11875"
     CDS             complement(5591..8809)
                     /locus_tag="VFC15_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM10897.1"
                     /translation="MTLQNKHFYAFGPFRLDSEKRVLVRDGTPVPLAPKAAEALLVLV
                     QNAGRLVDKDDLMKRMWPDAFVEEGNLNKNIFVLRNVLGKWDGGREYIETVPKRGYRF
                     VAPVSEVTHAEGDAQAQTSSGANLIGRKVSHYRVLEVVGGGGMGLVYKAEDLKLGRRV
                     ALKFLPEELVTDSLTLQRFEREARTSSSLNHPNICTIHEVEEHEGQPFIVMELLEGET
                     LREVISKAQVSAGGERSQLPMEKLLDIAIQITDGLDAAHQKGIIHRDIKPANIFVTIL
                     GQVKILDFGLAKLATAASEVEAEELAEAGAHGTQIRTKHESALEHSLTRTGMSMGTAG
                     YMSPEQARGEELDPRTDLFSFGLVLYEMATGQRAFSGNTAAILKDAILNHTPARVHDL
                     NSTLPPKLEQIIDKALEKDRERRYQSAAEMRAGLEILGDQTQHGLVRRHWKLFLSAAI
                     VLAAVVGGVVYWRSHRTVQLSERDTIVLADFANSTGDPVFDGTLKQALSIQLEQSPFL
                     NVLSDKQVSDTLKLMQHPVTERLTQTTAQAVCLRANSRAVLEGAIVSIGEGYQIGLKT
                     VDCQSGDTLATAEAKSTDRSKVLSSLSEAASHLRQKLGESRDSVQGFSIPLEEATTSS
                     LDALQAYTQGRKKQAEQGQAAAIPYFKLAVELDPNFADAHVALGTAHFGLYEYSQAAA
                     SLKKAFDLRDRVSQRERFFIETNYYNLVTGELEKFRDTLLEWARTYPRDYLPHTNVST
                     CYRFTGQFEKAVSEARLGLDLSPDNVGAVYTLMIGYIRMDRFDEAKRVYEEARARKLD
                     SPLMHYGRYLVAFVEHDDATMRDLIESAKGKPLTEDLMLMAQAGVQSYHGRLKQAREL
                     SGAATDLAAKAGSSDRAALWKTWVALWEAEIGNPERARSLTSEALALSTDQDVSISLA
                     LAFATSGDLVHAKKIADQLAHEHPLDTGIQRYQVPMTRAVIALKLNRPYEAIDALSAS
                     LPDDLGPSALMPAYLRGLAYLQAGNGRQAAGEFQKVIDHPGIVEMEVRGALSHLQLGR
                     AQAMMGDKDAARKSYQDFLTLWKDADPDIPIYRQAKAEYARL"
     gene            9485..10846
                     /locus_tag="VFC15_11880"
     CDS             9485..10846
                     /locus_tag="VFC15_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10898.1"
                     /translation="MSITLAAQAPSQQLRYTVFDMGTLGGPQSNVTAFAAVVNSQQAM
                     VGGADTSTRDPNYPNFNGLLSQDPFTQHAVVTQHGTRTDLGTLPGGNSSFAGWINSSG
                     TVVGSSENGSIDPITGTPEVRAVLWSHGTITNLGTLGGNESVALAVNTAGQVIGAAAN
                     AVPDNSSILGWGTQTRAFVYQNGAMQDLGTLGGTDAIAYINNDAGQVGGFSYTASGAG
                     DPFVWTNGNMKDLGTLGGTSGSVNAINSTGQATGGSNLAGNGASHAFFWDGNTLNDLG
                     SIYGFRGGFSVGIWLNDAGAVVGQSQVSPVANHAVLWKNGATTDLGVLPGDDCSIAWG
                     VNSGGQVVGASQICGGLAQHAVLWQNGQMFDLSNLLHGVKLVSAFYITDSGAIAAVGN
                     LAAGGNGVITYTSKQHAYLLIPPGSDDKKGSLPATQNTELDTVNQMREMVRQRFHADI
                     QPN"
     gene            10992..12539
                     /locus_tag="VFC15_11885"
     CDS             10992..12539
                     /locus_tag="VFC15_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10899.1"
                     /translation="MKSRTWMWTTAVYLFAALAMPALTAAQDNSSPEHRYKHHQYKLI
                     DIGTLGGHYDFFNFSGAQNNLLSSKGVVAGSSDTTMVDPYCFGNPDCFVEHAFQWQDG
                     ILTDLGALPGGANSNAFAINARGVIAGLAQNGAIDPLLLSSPPPWGVQVMHAVTWEHG
                     NITDLGTLGGYLSVAQAINDKGQVVGLALNDIPDPVSAFGLYVGTQMRAFLWRNGAMQ
                     DLGTLGGPDAWAYLLNQRGQVGGIAFTNSVINPATGVPTTDPFLWDAGNMQDLGTLGG
                     TYGFANGLNARGQVVGQSNLAGDLTFHPFSWTKSGGMRDLGTFGGDNGQANALNDAGE
                     IVGKADFPGSQIHDGFVWNHGVMTDLGTVDGDPCSDANGINSAGQVVGTSSNCHMAVH
                     AFLWENGGPMVDLNTLIVPLQPSVRVDGSDSYINDQGEILSSGVLQNGDVHAFLLIPC
                     DDKHPGQCEDYSMIEGAAPQISAPAAEFPAVMKQDGGSPVSPVERFRTMMRQRYHLPG
                     QPAAPRD"
BASE COUNT         2772 a         3615 c         3782 g         2798 t
ORIGIN      
        1 cagtttgatc ggctcggcga aactcaacgg gcttgatccg gaagcctacc tgcgagaggt
       61 gctgacgcgc atcgccgacc accccatcac tcacatcgag gagttgctgc cttggcagat
      121 cgccgcaagc ctaccgcccg cgatcgaacc ggccgcgtaa acaagaatgc agccgagaag
      181 aacttcgccg gtatttcctt gtccctgccc aacatcgcgg aaccgatcga acccggcgaa
      241 atcacggtcg gcgtccttta tccactcggg tgtgtcgccg ttgccttcgc ctttttgatt
      301 ggagggttgc ccgttcactt ctgcttctcc aaattgttca gttcaatgac ggctggaacg
      361 tactcaataa agtcctttgg acacccgtcg ctggcgctgc gaaacagagc cagagcttcc
      421 gggtgctcgc cgcgcagtgc gcgatactca cccacgtaga agctatattc acacttctgg
      481 tcgctgtctc cgccagtcgc ccggaaggta tcgtccagcg tactttgacc aagataaagt
      541 tggatcagcg gccccggcca ttttgacaag tccaagcccg cagtattcat tttcaattca
      601 tcttttccat ccgctccagc cttgacgcgc gacaggtaaa gccagatcac accgtacaga
      661 tctgtcctat ccaaccgcag attctgacta aaatcctgct gtgcgtcaga gaagtgccct
      721 gaatagagat ttaccagacc ccggttcaga agcgcgcgcg gagttttcgg atccaggcga
      781 atggcctcgt tgtagtcctg aatggcgcgg tcgtagtcgc ctttcgcgaa gtaagacgcg
      841 ccgcggccat tgaaggcact cgcagatttc ggattcaggc gaatagcctc gttgtagtct
      901 agaatggcgc tgtcatagtc tccctttagg aggtaagccg aaccacggcc agcgaacgca
      961 tccgcagatc ttggatccag gcgaatggcc tcgtcgtagt tctggatggc acgatcatag
     1021 gcgtctttgt cgttagcggt gccgagtgaa atagcgggct tgccggccaa tctctcactg
     1081 atatgctcgc cgaggcggtg agcgaagtct ccggccgtat tcactagaac gactacggca
     1141 tgatcggcct tcggattgaa ggctgctaaa ctgctgtatc cggccgtttc gccatcatgc
     1201 caatagcctc cggtaacggg agcgtacagc cacgcaagtg caatacgcat gcctgccatg
     1261 gcgtctgcgc gaagcacatg agattgggca agcgccgccg gaagcgtctc accatttggc
     1321 gtcttggtag caccggaagg aagcttgtcc ggatgcaggt ttgcttgcag gtacgtgagc
     1381 atgtcgtcgg cggttgaacg aatcgctccc gcccctgcaa atgcaaccag atcccaggca
     1441 tgagcaggat gatgggcccc gtcgtaaccg gcgatgagcc tgagttgttg ttcggctgaa
     1501 agtgcaacga ttgtatcctt catcccaagt gggccggtaa tctcttcctg aagtaactgg
     1561 ggataggtag tgccggcgcg cacggccaaa gcctgcccaa gtagcgcaaa accaagattg
     1621 ctataaagga aggtagcgtc cgccggtttg gcgactccgt gttttgtcag gaaggcgtac
     1681 agatcggccg cccgatagtc ggcatacgga ttgctgccgt cgggggggtt gaagttgtcc
     1741 ggcatccgcg gcaatccaga gtgctgcgta gctaagtcaa gtaacgtgat ttcgttcccg
     1801 gtgggcttgg ctaccgttcc agctggtagt aactcccgta ctggttcatc gaacttcact
     1861 tttccttgcg cgacaatctg cgccagcaac agtccggtga aggtcttcgt gatcgagcca
     1921 atttcaaaga tggaatccgg cttagctgtt ccatagctaa agacctgtct ttcaccatgg
     1981 tctacaattc cgatggtgac acctgcccct gtcgacgggg cgagtgcccc gctcgttagc
     2041 gcctgcgata ggtctttgct caaaacagcc tgcaactggg ctgccggcac gggagccatg
     2101 gccgtatcgt aggtgggagg atggacaggt gggagtgaga tagcgaccgt ttgtttttcg
     2161 aagttcaacg gcaacgggtg gccttgattc catgtaccgg tcagtgtctt gccatcagcg
     2221 gaaagcgttc caccccagtg gccactaacg acaggaatgt caaaagagaa cttgtttcct
     2281 tcgagaagaa cgttcccaca ctctagtccc attgcgcgct gatccagact gtcgttggaa
     2341 cagaactctt cgcccgtgat gtcgcctttg acgatgagct gtattcgcag cgatttagag
     2401 ccgtctctca atgttcccaa ccaaatccca tcgaccgcag agggtttggc cgcgaataca
     2461 aacgtgtcgc ggctgaaatt caacggcact ggagtgcctt ggttccatat accggttagc
     2521 aacgcaccct ggctgcccac agttcccttc catgtaccgt tcacggccgg cacagcgaag
     2581 cttagagact gtccgtcaaa atgaaaatca gcgcatggaa tgcccgacgc accctgatca
     2641 ggactgtcca aagttccgtt taaagcgccc tccgtgtcga tcttcaggtg cagcttcaga
     2701 tggagaggac cgagcgatcc tacatagtct cccgcgacat ttggcttttg ctgtgcgaag
     2761 accaggggag aaatgaaggc gagcaaaccg caaaagctcg tcagcaacat cgagcgtcgt
     2821 agcatgcacc gtttatacac ccatcggtca agctcaccgt tgagttccat gctgcggatc
     2881 acaggccttc gcgcagaagc gttcgcgcga agagaggcgt caaagtcgag ggagagcaag
     2941 accggttagg ccagcgacgg tgagaagtgc ccaccaaacc cttggggtct ctatgacatg
     3001 cacggcaaac cgaaggcact aagtctatac gctttgtcaa cacggcctac tacggacgct
     3061 tacggattag caacgagaaa cgcctttgta tcgtatgcgt ctgggcaaca caccttttcg
     3121 accagtagcg tgtgtggtga agtggctgga tgaatggaaa acagaaaagg tcaggccgcc
     3181 gacgcacccg tgaggaaatt cagcagctag tggctgagtt tttgagcagt agcatgcgac
     3241 gcaccgagtt ttgtcggagt cggggtttga gcttcggcac gctgaatcgc cacctaatga
     3301 agcaacgctg gaagagaaag agtagaagag cttcttcgcc cggtcggttg gtgccggtgg
     3361 aattggccgc tggtaaatcg ccgaggcagc acgaaccgag ttgtgggctg gccgtggtgc
     3421 tgccaggcgg gcgccggatc gaggtacacc ctgatttcga tacgaatacg ttcgaacgcc
     3481 tggtcagtgc gctggaacgg gtgtaagccg tgtttggatt gggtccagcc acacggatct
     3541 atctggccgc cggagtcacc gacatgcgca aggggttcga gggactgtac ggtctggtgc
     3601 gggatcgatt gtcgtgtgag cctcttagcg gtcacctgtt cttgttctgc aacgcccagc
     3661 gcaatcggtt gaaggtcctg gtctgggatg gtagcggact ctgggtctgt gccaagcgtt
     3721 tagagaaagg gcgtttcacc tggccgcaat cgggcgatgc gcagggcaag gtcgtcttga
     3781 ctcaggaaga gctgtctctc ttgttaggtg ggatcgatct gtgcaaaacc aagcacaagc
     3841 ggtggtaccg gaaggctcca ccagaaggac atgctgccgc ataaattgct tgtgcgtcgc
     3901 ggtcgactct gttaaatcag tagcccattg tgagtgaagc tcccattcct tcagcaccag
     3961 ccgatctgga actcatcgca caactgaaga gccggctgca gtacgcggag ctaaggattc
     4021 gtgtgctgga agagcgtctg cggctgatgc gcatcgagaa gtatggagcg ggcggcgaaa
     4081 agctttccca agcacaaatg gaattgttcg aactcagccc cgtcgtcagt aaaatgatcg
     4141 agcaggcgga gagtgagtgc acgccggtgc ggcgcccgac gcacagatcc tgcaagcatc
     4201 cgggccgcca ggggttgcct gcgaatcttc ctcgcgtaga gcggatcctc ccttgcacac
     4261 cggatcagcg tgtgtgcaag cgctgcggca aagagactgt agtgatcggc tacgaggaga
     4321 gttcccaact ggatgtggag ccggcgaagt acttcgtgct ggtcacgaag cgagagaagc
     4381 gggcttgccg gtcgtgtgag gaacttggag tggtttcggc accgatgcca ccacggatca
     4441 tcgagaagtg cttggcgagt gaccgcatcg tcatcgacac ggtcatcagc aaatattgca
     4501 atcacacacc cctgtatcgg cagagcatga ttctgggaag agacatcggc ttggaaatca
     4561 gccgagcgac gctggacggc tgggtcctga aagtcggcga gttgctgatc cctatggtcg
     4621 ccgcaatgag gcgagaattg atcagcggaa gttacattca ggcggacgaa accccggtcg
     4681 atgtgcagat gcgcgaggga cgaggaaaga atcatcaagc ctacttgtgg cagtacggcc
     4741 gacccggagg aagtgtggtg ttcgacttcc gtctgggtcg tgggcgcgat ggaccgaggc
     4801 ggttcctggg acagttcgaa ggcattctcc aaactgacgg ctataccgcc tacgagcaga
     4861 tcggcgggac gagaatggta catgccgctt gttgggctca tgctagaaga caatttttcg
     4921 aggcggtgca gctaaatcca caagacccgg tagcaactcc gattgtggcg cggatggacg
     4981 aactgtttgc cgtcgacgcg gaagctcgcc gcagaggaat tggtcttact gggcgtcatg
     5041 ctctgcgtcg ggaaagagcc aagcctctgc tggacgatat ccgcagcaag atcgaggcag
     5101 cacagtccgc tgcattgccc tccagtgcac tcagcaaggc ctgccaatac gcgctaacac
     5161 tgtggaggaa acttacacga ttcctggaat atccggagct tgagctgagc aataaccttg
     5221 cggaaaattc aatgcgtccc gtggcattgg gccggaagaa ctggatacac atcggcagtc
     5281 cacaggcggg acccaagatt gcggcgattc tctcggtcgt ggaaagctgc cgcaggttga
     5341 aactcccggt gcgcgactac ttggccgcag ttctccccgg gtttgccgat cttccgatcc
     5401 agcgcctccc agaccttact cccgctgcgt gggtcgtcca gcattcttag actcaagcga
     5461 ccgggatgtt agcgtggagg cgatcttgcg acaataaccg catagtttct tgtttgtgct
     5521 acgtccataa ccccgttcgt cacaacctgg gtgcggggtt tggcaaagcc aaaagtcctg
     5581 gatccggtgg ttagagccgc gcgtactcgg ctttggcttg ccggtagatg gggatgtcgg
     5641 gatcggcgtc tttccagagg gtgaggaaat cctgatagga tttgcgcgcg gcgtccttgt
     5701 cgcccatcat cgcctgcgcg cgtccaagct gcaagtgtga caaggcgccc ctcacttcca
     5761 tttccacgat tccgggatgg tcgatgacct tctgaaattc tcccgccgcc tgacggccgt
     5821 ttccagcctg caggtaagcc agaccgcgaa gatatgcggg catgagcgcg gaagggccaa
     5881 gatcgtccgg aagggaagcg ctgagagcat cgatggcttc gtatggtcgg ttcagcttta
     5941 gcgcaattac ggcgcgagtc attggaactt gatagcgctg gattccagta tccagcgggt
     6001 gttcgtgtgc gagttggtcg gcaattttct tagcgtgcac cagatctcct gacgtggcaa
     6061 aggctagcgc caaactgatg ctcacgtcct ggtccgtact cagagccaag gcctccgagg
     6121 tgaggctgcg ggcccgctcg gggttgccaa tctcagcttc ccacaaggcc acccatgtct
     6181 tccacagcgc tgccctatct gacgagccgg cctttgctgc caagtcggtc gctgcccccg
     6241 acagctctcg cgcttgcttc agcctcccgt ggtaggattg cacgccggcc tgtgccatca
     6301 acataagatc ttccgttagt ggcttacctt ttgccgactc gatcagatcg cgcatggtgg
     6361 catcgtcgtg ctccacgaag gccaccaggt aacgcccgta atgcatcaat ggtgaatcca
     6421 gctttcgggc acgagcttcc tcatagactc ttttcgcctc gtcgaagcgg tccattcgaa
     6481 tatagccaat catcagggta tataccgcgc caacattgtc cggtgagaga tcgagcccca
     6541 ggcgggcctc actgacagct ttctcgaact gccccgtgaa tcggtagcag gtgctaacat
     6601 tggtatgcgg aaggtaatct ctaggatagg ttctcgccca ttccagaagc gtgtctcgaa
     6661 acttctcaag ctcaccggtt actaaattgt aataattcgt ttcgatgaag aagcgctcgc
     6721 gctgactcac tcggtcgcgt aagtcgaagg ccttcttgag gcttgccgcc gcttggctgt
     6781 attcgtatag gccaaagtgt gcggttccga gagcgacatg agcgtcagcg aagttcggat
     6841 ccaactcaac ggcgagcttg aaatagggaa tagccgccgc ctgcccctgt tcagcctgct
     6901 tctttcggcc ttgcgtataa gcctgcaatg cgtccaggga agaggtggtg gcctcttcca
     6961 gcgggatact aaacccctgt acggaatcgc gcgactcgcc aagtttctga cgcagatggc
     7021 ttgccgcctc ggagagggag ctcagtacct tgctgcgatc tgtcgatttc gcctccgcag
     7081 ttgcgagtgt gtcgccggac tggcagtcca cggttttcag gccaatctgg tagccctccc
     7141 cgatcgaaac gatggcgcct tccagcaccg ctctgctgtt tgcccgaaga cagaccgctt
     7201 gcgcggttgt ctgtgtcagg cgctcagtaa cgggatgctg catcagcttc aaggtatcgc
     7261 tcacttgctt gtcggagagg acattcagaa acggcgactg ctccaactgg atagacagcg
     7321 cctgctttag cgtaccgtca aagacgggat cgccagtgga gttggcgaag tcggccagaa
     7381 caatcgtgtc tctctccgag agttgtacgg tccggtgcga gcgccagtaa acaacgcctc
     7441 cgacgacggc cgccagcaca atcgcagcac tcaagaaaag cttccagtgg cggcgaacca
     7501 acccgtgctg ggtttgatcg ccgaggattt ccaggccggc acgcatttcc gcagcggact
     7561 ggtagcgccg ctcgcgatcc ttctccagtg ctttgtcgat gatctgttcc agcttgggcg
     7621 ggagcgtgga attgagatca tgcacccgag cgggagtgtg gttcaggatt gcgtctttga
     7681 ggatcgccgc tgtgtttccg ctgaaggccc gctgcccagt cgccatttcg tagagaacca
     7741 ggccaaagga gaataggtca gtgcgcgggt ccaactcctc gccccgagcc tgttccgggg
     7801 acatgtaacc cgccgtaccc atcgacattc cggttcgggt caggctgtgc tcaagggcgg
     7861 attcgtgctt ggtacgtatc tgagttccgt gagccccggc ctcggctagc tcctccgctt
     7921 cgacttcgct tgcagcagtc gccagcttgg ctaatccgaa gtcgagaatc ttgacctgcc
     7981 ccagaatggt tacgaagata ttcgctggct tgatgtcgcg atggatgatg cccttctggt
     8041 gggccgcgtc caacccgtcg gtaatttgaa tggcgatgtc gagcaatttc tccattggta
     8101 gctgcgatct ctctccgccg gccgagactt gcgctttaga aatcacctcc cgcaaggtct
     8161 cgccctccag caactccatc acgatgaaag gctggccttc gtgctcttca acttcatgaa
     8221 tggtgcagat gttgggatgg ttcagtgatg aagacgtgcg cgcctcgcgt tcgaatcgct
     8281 gcagcgtcag cgagtcagta accaactctt ctggcaggaa cttcagcgcc acgcggcggc
     8341 ccagcttcag gtcctccgcc ttgtacacca gacccatgcc gccgccgccc acaacctcca
     8401 gcacgcggta gtgcgagact tttctcccga tcaggtttgc gccagaagag gtctgagctt
     8461 gagcgtcacc ctcggcatga gtcacttcgc tgacaggagc tacaaaccgg taaccccgtt
     8521 tgggcacagt ctcgatgtat tcccgcccgc cgtcccactt gcccaggacg ttccgcaaaa
     8581 cgaagatgtt cttgttgagg tttccttcct cgacaaaggc gtcgggccac atccgtttca
     8641 tcaggtcgtc tttgtccacc aggcgtccgg cgttctgaac cagtacgagc agggcttccg
     8701 ccgccttggg agccagggga accggggtgc cgtcgcgcac cagaacgcgc ttctcggagt
     8761 ctagtcggaa cggaccaaaa gcgtagaagt gcttattttg caaggtcata ccgactgcca
     8821 gaaatttgca ataactttgc cagaactttg gaaggacttt taaaccgcaa cggagttacg
     8881 gtgcgggaca agtggggtgg atgcggtctg gaaagccgtc cagcgcaaag tctattgcag
     8941 cccagtgctc tgccgcaaca ccgaatgtcg tcatagcgtt cggaaaggct gggcacggca
     9001 cctagaagcg gacggttgct gccttcctcc ctaccaatcg aaacccgaag gaggttacct
     9061 tccgtgtcgg tgtttttgaa gggacacggc ttctagctgt gccgttgaga gctaagagaa
     9121 gaatcgggct ccagcccctg agggaattcc gttttcttga tttgccacat caaggggtgc
     9181 cgttttcagc cgcgttgaag gcgaagtccc ccagaggcta aagccaaatg caaggaagtc
     9241 ctgtacggca cgtctgaagg cgtgcccctt caaagcaaaa cttggtcttt ccgcagtttt
     9301 ctgccgggac ggaatcggta atcggtatcc cctccgctcc ggcgcatgaa cttgctgcaa
     9361 gttctcatcg gcgattccgc tccgggcaag cccgaatcgc cattcacgaa gtaaagagga
     9421 gaagaacatg aaatgccgca catggatgtg gacgatacta gtgtcgttat tcgcagcgct
     9481 ggcgatgagt atcacgctcg ctgcgcaagc tccaagtcaa caactcaggt acaccgtatt
     9541 cgacatggga accctgggcg ggccccagag caacgtcacc gcatttgccg ctgtcgtgaa
     9601 tagtcaacaa gcgatggtgg ggggcgctga tacctcgacc cgagatccca attatcccaa
     9661 cttcaacggt ctactcagtc aagatccatt cacacagcat gctgttgtta cgcagcacgg
     9721 cacacggacc gacttgggga ccctgccggg cggaaacagt agttttgccg gttggataaa
     9781 ttcaagcgga acggtggttg ggagttcaga gaatggttcc atcgacccca taaccggtac
     9841 acctgaggtg cgggctgtcc tttggagcca tgggactatc accaacctgg gtaccctcgg
     9901 cggaaatgaa agcgtggcgt tggctgtgaa cactgcaggc caagtgatcg gagccgcagc
     9961 taatgccgtg cccgataatt cctccatcct tgggtgggga acgcaaacca gagctttcgt
    10021 ctatcagaac ggggcaatgc aggatttggg aaccctcggc ggtaccgatg ccatcgccta
    10081 tatcaacaac gatgccggcc aggtgggcgg attttcctac acagcctctg gagccggtga
    10141 tccttttgtc tggaccaatg gcaacatgaa ggacctcggg acgcttggag gcacatccgg
    10201 ctccgtgaat gcgatcaaca gcacaggtca ggcaaccgga ggttcgaact tagccggaaa
    10261 tggcgcctct cacgccttct tttgggacgg caacacgcta aacgacctgg gttcaatcta
    10321 tgggtttcgg gggggctttt cggtggggat ctggctcaac gatgctgggg cagttgtcgg
    10381 ccagtcgcaa gtttcgcccg tcgcaaatca tgcggtcctc tggaagaacg gtgcgacgac
    10441 tgatcttggg gtcctgcctg gcgatgactg cagcattgcg tggggcgtca attccggcgg
    10501 acaagtcgtg ggcgcatccc agatatgtgg tggcctggcg cagcatgccg tcctttggca
    10561 aaacggccaa atgttcgacc taagcaacct cctgcatggc gttaagttgg tcagcgcctt
    10621 ctacatcact gacagcggag cgattgcagc agtggggaat cttgctgctg gaggcaatgg
    10681 agtaatcact tatacctcca agcaacatgc atacctgcta attcctcctg gctctgatga
    10741 caaaaaagga tccctccccg ctacacagaa cacggagctg gacaccgtca atcaaatgcg
    10801 tgaaatggtg agacagcggt tccacgcgga cattcaaccg aactaactcg cggcgattgt
    10861 cctgccgggg cggaactgta gctcaatatt cccctttccg ctccggcgtt tgaacttgtt
    10921 gcaagttctc atcggcgatt ccgctccggg caagcctgaa tcgccattca cgaagtaaag
    10981 aggagaagaa catgaaatcc cgcacatgga tgtggacgac cgcggtgtat ctattcgccg
    11041 cgctggcaat gccggctttg acggcagcgc aagacaactc ctcgccagaa cacagataca
    11101 agcaccatca gtacaagctc atcgatatag gaaccctcgg cggtcactat gactttttca
    11161 acttcagtgg cgcgcagaat aacctgttga gtagcaaagg agtggttgcc ggcagctcag
    11221 atacgacgat ggttgacccc tactgctttg gcaaccccga ttgcttcgtc gagcatgcgt
    11281 tccaatggca ggacggcatc ttgactgacc tgggtgcact tccgggaggc gctaacagca
    11341 atgcttttgc gatcaatgcc cgtggggtca tcgccggttt ggcgcagaac ggggcgattg
    11401 acccgttgtt gctgtcatcg ccacctccgt ggggcgtgca ggtaatgcac gccgtgactt
    11461 gggaacacgg caatataacc gaccttggca cgctgggagg gtatctgagc gtcgcgcaag
    11521 caatcaatga caaggggcaa gtggtcggat tggcactgaa tgacatcccc gaccctgttt
    11581 ccgcgttcgg tctttacgtt gggacacaga tgcgggcgtt tctatggagg aacggggcga
    11641 tgcaggattt gggtaccttg ggaggacccg acgcttgggc ttatctcttg aatcagcgcg
    11701 gtcaggtggg tgggattgcg ttcacgaact cggtgatcaa cccagccacg ggagtgccaa
    11761 ccacagatcc tttcctttgg gatgccggca acatgcagga cctcgggaca ttgggaggca
    11821 cgtacggctt cgcgaatggt ctcaacgcac gaggtcaggt tgttggccag tcgaacctcg
    11881 ccggagacct gacgtttcac cccttctctt ggaccaagtc tggaggcatg cgggacttgg
    11941 gtaccttcgg tggagataac ggacaagcaa atgcgctcaa tgatgccggg gagatcgtcg
    12001 gcaaggcgga tttcccaggc tcgcaaatac acgatgggtt tgtctggaat cacggggtga
    12061 tgacggatct cgggaccgtg gacggtgatc cgtgcagtga tgccaatggc atcaactcag
    12121 cgggccaggt cgtgggcaca tcatccaact gtcacatggc tgtgcacgcg tttctgtggg
    12181 agaacggcgg gcccatggtc gatctgaaca ctcttattgt tcctctccaa cctagtgttc
    12241 gggtggatgg gagcgattcc tacattaatg atcagggaga aattctttcc agcggagtac
    12301 tccagaacgg tgacgtccac gctttcctgc tcatcccgtg cgacgacaaa catcctgggc
    12361 agtgcgaaga ttactccatg atagaaggag ccgctccgca gattagcgcg ccggcggcag
    12421 agtttccggc cgtgatgaag caagacggcg gatcgcctgt cagtcctgtt gaacgcttcc
    12481 gcactatgat gcgccagcgg taccaccttc ccggccaacc agccgcaccg cgcgattaac
    12541 aagctcccac ccaagcaaga cagcttgggt gggcaccctg cattttgccg gggcggaact
    12601 gctaatcggt atccccctcc gctccggcgc ttgaacttgc agcaagttct catcggcgat
    12661 tccgctccgg gcaagcctga atcgccactc acgaagcaaa gaggaggaga acatgaaatc
    12721 ccgcacctgg atgtggatga ccgtagtgta tctattaagc cgatccacta ctgtccggtt
    12781 aaaagtcgaa gagaatcagt tggttgaggg tccaggtaac gcgactaggc gtcctcgaag
    12841 aaggcacgga gatagccctt gaacgtatcc gtctcgccgc ggatcgttcg cggtgcgatg
    12901 tcgaaccgcc gcaaaagacg cgcgagtgtg ttggcggtaa gctcccgccc tttgttgcat
    12961 tcaggcc
//
LOCUS       DASRKK010000518         6123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_629027, whole genome shotgun sequence.
ACCESSION   DASRKK010000518 DASRKK010000000
VERSION     DASRKK010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629027"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFC15_11890"
     CDS             complement(<1..528)
                     /locus_tag="VFC15_11890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM10900.1"
                     /translation="MRIAQIAPLHERVPPRLYGGTERVVSFLTEELVRQGHEVTLFAS
                     GDSETSARLVPCCEMALRLNPSVRDHLPYHMIMLENVRQRIDQFDILHFHIDLLHGPL
                     VRAIANWTLTTLHGRLDLPDLVPFYQTFRELPLVSISNDQQRYLHNVNWLGTVYHGLP
                     RDLLAFQSRAGGYLAF"
     gene            complement(641..1402)
                     /locus_tag="VFC15_11895"
     CDS             complement(641..1402)
                     /locus_tag="VFC15_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HZM10901.1"
                     /translation="MVIIDVGRFGAFVLDLDGVITRTASIHARAWKQLFDQFLAQRAA
                     QSATPFLPFDPETDYRRYVDGKPRIVGLQSFLASRGINVRLGEPGDQPEQETAHGLAK
                     RKDLYFLKLLAVVGVQVFDSAPAFLRDARGRGVRTAIASSSHHCAEILHAARLTALFD
                     ARVDGIDLDRLSLTGKPAPDMFLEAARRLNVPPAHAVVFEDATAGVEAGRAGSFGLVI
                     GVGPAARAPDLLKHGADDVVANLGEVRLEGTAHLP"
     gene            complement(1411..2259)
                     /locus_tag="VFC15_11900"
     CDS             complement(1411..2259)
                     /locus_tag="VFC15_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZM10902.1"
                     /translation="MTSAQTVLVPLDGSVRATAAVPVARGLAELFHATLAVLHVSEET
                     LESAALVDRMKLSSQDLRGLVVDQRTGAAAARIVQEATERHAALIVMCPRTRMDARPR
                     TFGSVAEAVLRAARCPVVLVPPTRGRRDWALRQLLLPHDGTPTSAATIGPATDLASKA
                     AAKLVVLHVATAGAERPTEHGTLVSPRYVDQPQHEWPAWAEEFLDRLRAVGHATDGVD
                     IRLVLAQGEAGSAIVDFARRNDSDLIVLGWRGLLEHDRARTMQYVIRESICPVIVFRL
                     DRDDGR"
     gene            complement(2256..4736)
                     /locus_tag="VFC15_11905"
     CDS             complement(2256..4736)
                     /locus_tag="VFC15_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HZM10903.1"
                     /translation="MKWAAQVSPEASPRILLLSCDSCGRANWSSRSSGPRCSLHSANS
                     DCIDDTTIRSSKDMGALPFSPSPDPDWLLLEEGVDLAREREIESIFAIANGYIGARAS
                     IGEDGRFSNPSTFAAGVYVTDASPELGTRLAVLPNWFHVEVRVEDQRLSLEAGRVLDH
                     RRLLDLRQGVMWRDWRQQDPRGRITRLFYLQLASLADRHILLQSVTVTAENYAGRISL
                     TTRLVPSGEIGMETAHIGAEPAVLMRVSGIDVAIASTSMLHCSNSSRTLTHEERSDGG
                     EERWSWETGLGEAVRLDRIITVFTSRDGVNPAKAALSHLATTRTRGFPPAAQAHFDAW
                     HRKWGAADVRIVGDKQAQRALRFACYHLISAVNPADEYVSICARGLTGEAYRGHVFWD
                     TEIYMVPFYVFTDPLSARSLLMYRYHTLDAARRKAKEHGYEGALYAWESADTGDEVTP
                     RRAVMPDGRVVAILTGEREHHISADIAYAVWQYWRASGDDGFMLAAGAEILVETARFW
                     ASRAQVEPHGRAHIRQVIGPDEYHEMIDDNAYTNAMAIFNLERAADTIEILQREQVAD
                     WQRLCTNLDLAEKEPATWRALAGQLATGFDSATKLFEQFAGYFQLDEVDVVRQRASAM
                     PIDAYLGPERVRRSKAIKQADVVALSAVLWDQWPVAVHEANFRYYEPRTAHGSSLSPA
                     LYALVAARLGDGALAQAYFRQAAEIDLANNMGNAAGGVHMGALGGLWQAAVFGAAGLR
                     AREDGIALDPHLLPGWTEMAFPVQWRGRLLRLRLEADRRCIEVNVESGGELCLSVPEG
                     PVCLARPGRRYVLRGDHSGWGSWEEVGQ"
     gene            complement(4696..5112)
                     /locus_tag="VFC15_11910"
     CDS             complement(4696..5112)
                     /locus_tag="VFC15_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZM10904.1"
                     /translation="MTYHFSSTVDMKFDDAVACLKEVLKQHNFRVVSEIDMKDNFKKA
                     LNLEFRPYLVLGACNPQLTYRALQAEDKIGTMLPCTIVVQQHDDGRVEVSAVDPVTSM
                     QAITNVVVNQVAEELRSHLKSVTDEVGRTGQSGGVT"
     gene            5191..5700
                     /locus_tag="VFC15_11915"
                     /pseudo
     CDS             5191..5700
                     /locus_tag="VFC15_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1316 a         1820 c         1800 g         1187 t
ORIGIN      
        1 gaaggcgagg taaccaccag cacgcgattg gaacgcaagg aggtcgcgcg gaagaccatg
       61 gtagacagtg ccgagccagt tcacgttgtg cagataccgc tgctgatcat ttgagatcga
      121 gacgaggggg agttctcgaa aagtttggta gaatggcacg agatccggca aatccaatcg
      181 gccatgcaaa gtcgtcaatg tccaatttgc aatggcgcga accagcggac cgtgaagtag
      241 atcgatgtgg aaatgtagga tgtcgaactg atcgatcctt tgacggacgt tttccagcat
      301 aatcatatgg tagggcaaat ggtcccgcac ggaaggattg aggcgcaacg ccatctcaca
      361 gcatggcacg agccgtgccg acgtttcgga atctccgcta gcaaagagcg ttacctcgtg
      421 cccttggcgg acgagctcct cggtcaaaaa cgaaacgacc cgctctgtcc cgccataaag
      481 ccgcggcgga actcgttcgt gtagtggggc gatttgagca atcctcatgg tcaaatcctc
      541 tgtaaacata aaattcgagc ggaaagcgaa tgacttgatt tggatcaaat ggaggataag
      601 gaagattgcg attgaagggt gccgttacgc ccgcgcggct ttaaggcaaa tgggccgtgc
      661 cctccagccg gacttcgcct aggttggcaa caacgtcgtc cgccccatgc ttgagcaggt
      721 ctggcgcgcg cgccgcaggc ccgacgccga tgacaaggcc gaaactgccg gcgcgacccg
      781 cttcgacgcc agccgtcgca tcctcgaaca ccacagcatg cgctggaggt acgttgagac
      841 ggcgtgcggc ttccagaaac atgtcaggag ccggcttccc tgtgaggctt agtcgatcaa
      901 gatcgatgcc gtcgactcgt gcatcgaaca gcgctgtcag cctcgcagcg tgcagaattt
      961 cggcgcaatg gtgactggat gaggcgatgg ccgtgcgcac acctcgtccc cgagcatccc
     1021 ggagaaatgc tggtgccgaa tcgaacacct ggacccccac aactgccaga agcttcagga
     1081 aatagaggtc tttgcgcttg gcgagcccat gagccgtttc ctgctcaggc tgatccccag
     1141 gttctcccaa gcgcacattg atccctcggg aggcgagaaa gctctgcaac cccacaatgc
     1201 gcggcttgcc atctacatag cggcgataat cggtctcggg atcgaagggg aggaacggcg
     1261 tcgcgctctg cgctgctcgc tgggcgagga attggtcaaa cagctgtttc caagcacggg
     1321 cgtggatgga agccgtgcgc gtgatcaccc cgtccaagtc gaggacgaag gcgccgaaac
     1381 gtccgacatc gatgatgacc atagcggagg ttacctgccg tcgtcgcgat cgaggcggaa
     1441 tacaatcacc ggacagatag attcacggat tacgtactgc attgtgcgcg cgcggtcatg
     1501 ctcgagtaaa ccgcgccagc cgagaacgat gagatcacta tcgttccgtc gcgcgaaatc
     1561 gacgatagcc gagccagcct cgccctgagc gagcacgaga cggatgtcga ccccgtcggt
     1621 tgcgtgcccg acagcgcgca gccgatcgag gaactcctcc gcccatgcgg gccactcgtg
     1681 ttggggctgg tcgacatagc ggggggagac cagtgtccca tgctcagtgg gccgctcggc
     1741 accggccgta gcgacgtgaa gcaccacgag tttggctgcg gcctttgatg ccaggtcggt
     1801 ggcagggcca attgttgcgg cgctcgtcgg tgtcccatca tgaggcaata gcagttggcg
     1861 cagagcccaa tctctgcggc cgcgggttgg aggtacgaga acgaccgggc atcgcgctgc
     1921 gcgaagaacg gcctcggcca cactgccaaa tgttcgcggt ctggcatcca tccgtgtccg
     1981 tgggcacatg acgatcaggg ctgcgtgtcg ctcggtggct tcttgcacga tacgggccgc
     2041 ggccgcaccc gtacgctgat ctacaaccag cccacgcagg tcttgggatg agagcttcat
     2101 gcggtcgacc agcgcggcgg attccaaggt ctcctcggaa acatggagaa cggcgagggt
     2161 ggcatgaaac aattcggcaa gcccacgcgc cacgggcacc gccgccgtcg cgcggacgga
     2221 gccgtcgagg gggaccagca ctgtctgcgc agaagtcatt ggcccacctc ttcccaacta
     2281 ccccatcctg agtggtcgcc acgcaggaca tagcgccggc cgggacgggc gaggcatacg
     2341 gggccctcgg ggaccgagag acagagctcg ccaccgctct cgacattgac ctcgatacac
     2401 ctgcggtcag cttccaggcg caaacgcagc aagcgtcccc gccattgcac cggaaatgcc
     2461 atttcggtcc agcccgggag cagatgtggg tcgagcgcaa tgccgtcctc acgtgcgcgt
     2521 aagccggccg cgccgaatac cgcagcctgc cagaggccac ctagggcgcc catatgaacg
     2581 ccgccggcgg cgttacccat gttgttggcc aagtcgatct cggcagcttg gcggaaataa
     2641 gcttgcgcaa gagccccatc gccgagccgg gcggcgacga gggcgtaaag cgcagggctc
     2701 agcgaactgc catgggcggt gcggggttcg taataccgga agttcgcctc gtgcaccgcg
     2761 acaggccatt gatcccacag cactgcgctg agcgcgacca catctgcctg cttgatcgcc
     2821 ttcgagcgac gcacgcgctc cggtccgaga taggcgtcaa tcggcatggc cgaagctcgc
     2881 tggcgcacta catcgacctc atcgagttgg aaatagccag cgaattgctc gaagagcttg
     2941 gttgcagaat cgaaacccgt cgccagctgc ccagccagcg cacgccacgt cgcgggttcc
     3001 ttctcggcaa ggtcaaggtt tgtgcatagc cgctgccagt cggcgacttg ctcgcgctgc
     3061 aggatctcaa tggtatccgc agcacgttca aggttgaaaa tcgccatcgc atttgtgtac
     3121 gcgttgtcgt cgatcatttc gtgatactca tcagggccga tcacttgccg aatgtgggcg
     3181 cgaccatgag gctcaacttg tgcccggctc gcccagaagc gtgccgtctc cacgaggatc
     3241 tcggcacctg ccgctagcat gaagccgtcg tcccccgacg cgcgccagta ctgccagaca
     3301 gcgtacgcaa tgtcggcgct aatgtggtgc tctcgttccc ctgtaaggat ggcgacgacc
     3361 cggccatcgg gcatgaccgc gcgccgcgga gttacctcat caccggtatc ggccgattcc
     3421 caggcataga gggcaccctc atagccatgc tccttggctt tacgccgcgc tgcatcgagc
     3481 gtgtgatagc ggtacatcag cagagatcga gcagacagcg gatcagtgaa gacatagaat
     3541 ggcaccatat agatctcggt gtcccagaac acatgacccc gatacgcttc gccggtcagc
     3601 ccgcgcgcac agatcgagac gtattcgtcg gcagggttga ccgctgagat gagatgatag
     3661 catgcgaagc gaagtgcgcg ctgcgcctgc ttgtcaccca cgatccgaac atccgcggcg
     3721 ccccacttgc gatgccaggc atcgaaatgc gcctgcgcag cgggcggaaa gccacgcgtg
     3781 cgcgttgtcg caagatgact caaagccgct ttagctgggt taacaccgtc acgcgacgtg
     3841 aagacagtaa tgatacgatc taggcggacc gcttcaccga ggcctgtctc ccatgaccat
     3901 cgctcttcgc cgccgtcgga tcgttcttcg tgcgtcaacg ttctcgacga gttcgagcag
     3961 tgcagcatgg acgtactggc gatcgcgacg tctatcccag atacgcgcat gagcacggca
     4021 ggttcggccc cgatatgcgc ggtttccatg ccgatttcac ccgacgggac aaggcgtgtg
     4081 gtgagactta tccttcctgc gtagttttcg gccgttaccg tgaccgattg gagaaggatg
     4141 tgccgatcag cgagagaagc aagctgcaga taaaataatc gggtaatgcg tccgcgcggg
     4201 tcttgctgtc gccaatcgcg ccacatgaca ccctggcgca agtcgagaag tcgccgatga
     4261 tcgagtacgc ggccagcctc gagcgacagt ctttgatctt ccaccctgac ttctacgtga
     4321 aaccaatttg gaagcaccgc gagtctagtg ccgagttcgg ggcttgcgtc agtaacgtaa
     4381 acgccggcag caaatgtcga tggattcgaa aatcttccat cctctccgat tgaggccctc
     4441 gccccgatat atccattggc tattgcaaat atcgactcga tttcgcgctc tcgtgccaga
     4501 tccacaccct cttcgaggag gagccaatct ggatcggggg aaggtgagaa cggaagagcg
     4561 cccatatcct ttgaggacct tattgtcgta tcgtcaatgc agtccgagtt tgctgaatga
     4621 agagaacatc gaggtccgct cgaccttgac gaccagttcg cgcgcccgca gctatcgcaa
     4681 gacaaaagca atatcctagg tgacgcctcc ggactgacct gtgcggccca cttcatcggt
     4741 cacgcttttc aaatgggagc ggagctcttc agcaacctgg ttcaccacaa cattggtgat
     4801 cgcttgcatt gaggtgacgg gatcaactgc ggaaacctca acgcggccat cgtcatgttg
     4861 ctgcactacg atggtgcagg gtaacatcgt tccaatcttg tcctccgctt gaagcgctcg
     4921 ataggtgagt tgcggattgc atgcgccgag aacaaggtat ggacggaatt cgagattgag
     4981 ggccttcttg aagttgtctt tcatatcgat ttccgaaacc actcggaaat tgtgctgttt
     5041 gagcacctct ttcaggcagg ccaccgcgtc gtcaaacttc atgtcgacag tgctggagaa
     5101 atggtaggtc atgttcgcgg ccttcgcact ggaaccccgt ttagaatgct cattaaagct
     5161 ctcccgacag gcgaccatca tgatctaagt caagggatgg cctgcgagca agcctgcatc
     5221 acaatttatc tgccgaagcc gatgacctcg ggccttctcg ccaaaggccg cttcggcaag
     5281 caggacttcg tctatgttgc cgcggacgac gtttatcttt gtcccgccgg tgagcagcta
     5341 acctatcact acaccaatga ggaagagggc aagacactac gtcgctattg gaccactgcc
     5401 tgtcaggcct gcgtgctgaa gagcaagtgc acaacagcca aggagcggcg gatctcacga
     5461 tgggagcacg aagccgtcct cgaggctgtg caagcgcggc tcgatcggaa cccggataag
     5521 atgcgcgaac gccgccagac cgtggagcat ccctttagca cgataaaatc ctggatggga
     5581 gccactcact tccaaatgaa gacgctcaag cacgtcggta ccgaaatggc cctgcacgtc
     5641 ctggcctaca acatgaaacg ggtaatgcgc attctgggcg tcggcggatt gatagaagcg
     5701 cgatccgagc atgaaatcgg atttgccgat ccggccccgc ccttccaact aaaaccgcgt
     5761 ttttacacag tctggaccct gaccggacat ccggcgattg tgttcgccgg gcgctcacgt
     5821 tgacccagat gaattcctct gtctgaacca gcatatatgg cttctatggt ggtcctccat
     5881 accattacta cggcgggggt cttagcctga tactggtgat cgtccttttg ttcatgctgt
     5941 tcaggcgctg attatccgca acctcacgag aggacttctg gttgtgggca cacgactatt
     6001 gtgcccccgc caagtctaga gagcattcat gcccgcttct ccccgagccc ccaccttatt
     6061 ccgtgatcgc cagatggagg caccatgcac gaagtgcggc gcgaaaatga tgcttgctcg
     6121 cat
//
LOCUS       DASRKK010000519        16002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_238006, whole genome shotgun sequence.
ACCESSION   DASRKK010000519 DASRKK010000000
VERSION     DASRKK010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16002
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238006"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFC15_11920"
     CDS             complement(<1..1081)
                     /locus_tag="VFC15_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZM10905.1"
                     /translation="MSSAPSDRVSSNGLTVAAYAGDGDVLLAFSLDDSVLKQNDLAGF
                     SIQCTPPTGQPFYLQNRINFTQPVTSATAPQQHPWTSSQDAPFQKFHWVHFPPDVVPG
                     DFKYKVTARYCQGTALVDGPSGEVTVQLVKPQTGNFELGLTRAYVSSQAYVAKFQNKD
                     IRPQPKTLDYDTTSYQPQYEWLGFHARKMVFDLLKECVSDDSISVDMFAYDFDEPDIL
                     KMCESLGSRLRIFLDNAPLHTGTALEVQVHERLVKSAGAANVKQGHFKRFAHDKIIIQ
                     KKNGKATKVLTGSANFSIRGLYVQANNVMLFSDPTVAGQYEQVFESVFNDMGGYVKTP
                     LANQWFSFPNLGGGVPKFQVNFAPHP"
     gene            1644..2921
                     /locus_tag="VFC15_11925"
     CDS             1644..2921
                     /locus_tag="VFC15_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HZM10906.1"
                     /translation="MTEIVTNPDLSPYPAPRGSKPAYHAMVKPIGAICNIDCTYCYYL
                     HKKELLGSDSKFRITDEILEEHIKQYIEGQVRDEVVFSWQGGEPTLLGLEFFQRVVEL
                     EQKYKNPHQRIENDLQTNGTLLNDEWGAFLKQNGFLVGLSIDGPKELHDVYRVTRDGK
                     STFDKVFAAAQMLHRHGVPFNTLTVVNRVSAKKPLDVYRFLKNEVRPRQMQFIPCVEP
                     KVFRNVAPQMWDPETLPLYDSPAAHPGNADSVVTDWSIDPDDWGYFLCKVWDDWYRRD
                     MGKAFVNLFETAVGQWMGKESQVCIYHEFCGKGVAMEHDGSLYSCDHYVYPEYKLGNI
                     QETSSSRMVFSDRQKEFGFAKFSALPKRCRECEYLFACNGECPKNRLIRTPEGEPGLN
                     YLCSGLQKFWRHIDADVQDICRRIARGEPLKAR"
     gene            complement(3078..3974)
                     /locus_tag="VFC15_11930"
     CDS             complement(3078..3974)
                     /locus_tag="VFC15_11930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM10907.1"
                     /translation="MSSTEPDVPASSPDASPVPENGVSASSRGGGAIKQAERDWHDAF
                     FNEYALVAYPESLVDFQRIFQLVELTPFSEGGWNWWADLRREALDSVGDVRGLRVLDY
                     GCGFGKLGMYLSLCGAQVWGFDLSSPAIETANQVARRYGLTAQFEQMDAEDLRYADGS
                     FDLIIGFGVLHHVIKYPRAGAQLFRVLAPGGRAIFHETLWDNPLINLARRFTSEHADA
                     GDAPLTDKNIREFCRDFSQIRLEKRNLLYMLKRLAKLPYPDPGVPVRPRPFWQLMKTL
                     DAQLLRFAPLRRYCGEVIIYLA"
     gene            4204..4842
                     /locus_tag="VFC15_11935"
     CDS             4204..4842
                     /locus_tag="VFC15_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HZM10908.1"
                     /translation="MPTSNAVIDLSHNNQSVDFNKAKAAGILGVVHKATQGTGFVDPM
                     YAKRRKAAANAGLLWGAYHFGTGADPIAQAQSFLKVASPTAKDILVLDFEPNTTPPRN
                     SMTLVQARAFIGFVKNATGVMPGLYGGAYLQQQLGSASDSLLGACWLWWAQYRKAPAI
                     PPNWKAYTLWQYTDGHSGNPPYEVDGIGPCDRDLYQGTTDDLQKKWVTGTLL"
     gene            complement(4866..5315)
                     /locus_tag="VFC15_11940"
     CDS             complement(4866..5315)
                     /locus_tag="VFC15_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10909.1"
                     /translation="MHDSGESSMPNFCKKLVLLAAFLALSGLLPASAQKAQGADAGVP
                     KYDLHTETTVKGTVEEVKLPPKGSEIAHLMVKNETESVDVYLCPKSFLDDMGLTFSKG
                     EEIAVTGSKVKQNGADIVLAREVAKVNDKVTLRDNKGDPVWSWQKKN"
     gene            complement(5661..6872)
                     /locus_tag="VFC15_11945"
     CDS             complement(5661..6872)
                     /locus_tag="VFC15_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10910.1"
                     /translation="MKNSGSLSRRTLLKAGISTMAMAPLAGAKMSLAQLLPEATPVDT
                     TIPIVVGGRLLATSVAGVPPYVIESDRGTGFLPIYLRNAAIDPSLLGQHVTVGGTLTS
                     GACSPPALELVNPTSPVISTPPSPFIPDCGTTLQNTPLTSQEAAALEQILLAYIGALF
                     YNLDSSIQAMFADQATRALTDPTFANYIVDIPFISVEDDTQCPSLQCYSFSDFVDFAA
                     AGATSTNSTGTTALAIGAVSDECLRKILIELLCFLLGLIVAFVPRAAVAEMERQATAL
                     LTGPRIRQAVLNIVAAAQRVIAGTFRASQFIDVLISAMHAMAGPLGLAFLAVIRTLRW
                     SQYVLALAASAGVIIKAIIVIAKGAMFIWKLIDIVKTCLGSSSACPVPLGDKPAGTFA
                     DLTAAAALPGY"
     gene            complement(6994..7593)
                     /locus_tag="VFC15_11950"
     CDS             complement(6994..7593)
                     /locus_tag="VFC15_11950"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM10911.1"
                     /translation="MKTKKIASRFSLGILALVSLAALQLSPAAYADPVKTVTFACTKC
                     TGKVNETVALNKITKADGTAGNVTDQNNLFNGKQFDFTFDTAAKTVSLSGNGINMTGT
                     IANPVFTVGQQTDDANFVANWNVGTLPPAVKGALGWKNGMNVTSPSVQVLWDHGGGNV
                     QSVNLDMVSQNPEPASLLLFGSGVAGLGTYLRRRLIRRS"
     gene            8328..8939
                     /locus_tag="VFC15_11955"
     CDS             8328..8939
                     /locus_tag="VFC15_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZM10912.1"
                     /translation="MADTYRLLRFGVFELNLDLEEIRKNGTPLKLPPQPFKLLALLAS
                     RSGQVVIRDEIQEQLWGSDTFIDFEQGVNKCIKQIRTALSDNADNPLYLETIPRRGYR
                     FLAPVRTKIIPAPPPQVKEASSGLLANVVAQVRAQVESASKTAGDVSQPAADATVEGA
                     AHETSPTAPASTQSMRTRLIWALVPLAILFAAVLYWYLSRAVR"
     gene            complement(9059..9739)
                     /locus_tag="VFC15_11960"
     CDS             complement(9059..9739)
                     /locus_tag="VFC15_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10913.1"
                     /translation="MSDRNSWKMLHLLYTLKPGVVGLTAGQLASAGVDCGPDTIQPLI
                     DCKAVLVDASGVHSLSEPSRKILGCCLVANRRWSGKDVLVDYPEVFVVMPFREPWSIS
                     VFEEMIRPAVEAVRFQCVRGDAPPRVGDLTQNIWSAIARAGLVVAEVSVPNPNVFYEI
                     GLTHALGKDCFILKQRDASVPADFGGSHYYEYELANLKAGRESLQRELGAWATENSVE
                     GVRDISKS"
     gene            complement(9778..14466)
                     /locus_tag="VFC15_11965"
     CDS             complement(9778..14466)
                     /locus_tag="VFC15_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZM10914.1"
                     /translation="MSAAPRYPGIRVTANGNQLVSYHTETRIADAGVFYPITPSTEGG
                     ELYQQSYAEGHLTVFGGSTLAIETEGEHAAQGGAIAHSVCGKRVVNFTSGQGVLYGIE
                     QYYHAPGKCSTMVLEVVARALTKHALNVHCGHDDVYGALDTGWIILFGKDAQQTADQA
                     LILRRVTELSLTPGMNCQDGFLTSHLERTFYRHEADLIREFLGAPDDLIDCPTESQRV
                     LFGPKRRRVPRMIDLTNPVLLGPVQNQEHYMQGIVARRNNFTEPILPFLEAAYAEFGM
                     LTGRHYGLVTCYKTDDADTVFVSLGSAAENIEAAVDYLREHRGVKVGSIHLNVIRPFP
                     EVAVVNALAGKKNVIVLERTDEAMAGDNPMGRDIRTALNKALQTDGHPAVAGLPAITP
                     AQMPLLFSGIYGCGSRDFRPEHVFGAYEFATGTRPRKDGKHASDGTSFFVLGVDHPYE
                     VKSEDTPSLLPEGAVAVRFHSIGGWGAITTGKNLGAIIGDLNDLLFERDKIVDELGNP
                     KEIIHVSANPKYGSEKKGAPTAYFMVAAPKRIRVNCDLRHVTVVLCCDPKAFTHTNPL
                     DGISQGGCLVWESDQEGELAWEQLPLWARKQIIEKNVRVFTLPGFQIARKATDRADLQ
                     LRMQGNAFLGAFFRVSPLLGEFRITHEQFLEVVHNQYVKKFGRLGSAVVKSNMEVMTQ
                     GFEQVREIRVGELNALDRSSLRGIALFPTFSEDGDACGIGCRSHPAPEGQAERTPVTQ
                     IASFDGQFRAGLGYNQPSNAYASVGVMAASTGDTASKYVARRETPLFIPENCTQCMEC
                     IAVCPDTALPNCAQDLQTLLNTAVTRYVTDQGERRKMMDSLPEIDKRTRQRMADDVAK
                     KVDTPLPEILREVMTAVDGFSPEAKRQFFAIMDKVPMAYQKVNAIFSTPERKKPGTGG
                     IFSIFVTDLCKGCAACVTACGEHEALKMVDETEMVNAEHETGTAFLNLLPDTPQKYLG
                     LYNDAKPQDSKTATLRNMLMVRHNYDAMVCGDGACAGCGEKSILRAIAACTEAYMRPL
                     YHAKADRLRTKAIELEQTGVEKLAALKQRNPEEYDLFRQCVAHLLMGLGGEDDRDTKS
                     RMANHGAISDTEIVNAICAVMRQEAFNHRDLQAVDGRLANGMCVMAMTAHTGCNTVYG
                     STPPNNPHPYPWMNSLFQDGVTIGWLLGESFIVDHGRRSVVPERLADALLNRETEVMT
                     PLDFYSYTHFSDLLMTDQEIIELPKVWAIGGDGGMGDIGYQNTSKVVLQNRPNVKVLM
                     LDTQVYSNTGGQNSDSTPMLGGSDMNVFGEATQGKSAEKKTVAETFLAGHGSPFVAQI
                     SIANAPKLFRAILDGIEYRGTAFLQCFTTCQPEHGVGDDMALTQAQRVRDSRCSPEFV
                     FNPRLGETYPEALDLKGNPSLDLDWYEAKSKGSDEVARYTIAHWCATEARFRNHLKKI
                     KEEQAATLVPLDNMLLRLTQQDVVYRRYLQPEHRSFIPDFGVYIKLQGENGKPEYRAI
                     SRQLVLFCVERRKAWRLLQSKAGIINKDYVAQKALLAEADAGKISKAELFVRCEQMVT
                     ERQQPAVLAH"
     gene            14698..15987
                     /locus_tag="VFC15_11970"
     CDS             14698..15987
                     /locus_tag="VFC15_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HZM10915.1"
                     /translation="MAEKQVCFVVMGFGKKTDYPTGRVIDLDKSYQYIIKPAAEEAGL
                     ECIRADEIIHSGTIDVPMYEQLLNADVVVADVSTSNCNAFYELGVRHALRPYTTITIA
                     EDKMVFPFDVNHIAIRKYQHLGDGIDFGEVMRMKAALKEAIQTIAAKPTDDSPVYTFL
                     KDLRPPVRGAKEAALKFAMAAISDGPSAGQDPANTATVSTLMAQAQAALDKSDFLTAK
                     SLLAVVRSMIPNDPYVAQKLALATYKSKLPDPTQALKDAHAILTELNPEVSTDTETLG
                     LWGAVHKRLWEITADRSYLDISIFSYEKGFYLKNDYYNGINLAYLYNERAAISEAPES
                     VADFVCAERARKRVLTICDSLLQSGRTLPGQDEYWIRATMAEAWLGLGDVNKSKESLD
                     AAFALQPSPSQWMRDSTQEQLQKLEKLLASSPLKKTN"
BASE COUNT         3335 a         4522 c         4851 g         3294 t
ORIGIN      
        1 cgggatgagg cgcgaaattc acctggaatt ttggtacgcc accgccgagg ttggggaagg
       61 agaaccactg gttggccaga ggcgtcttca catatccgcc catgtcgttg aagacggatt
      121 caaatacctg ctcgtactgg ccggcaaccg tgggatcgct aaacagcatt acgttgttgg
      181 cctgcacgta gagcccacga atggagaagt tggccgaacc tgtgagtacc ttggtcgcct
      241 tgccgttctt cttctggatg atgatcttgt cgtgggcgaa acgcttgaag tgtccctgct
      301 taacgttggc ggctccggcc gacttgacca ggcgctcgtg cacttgcact tccagggcgg
      361 tgccggtgtg caggggcgcg ttatcgagaa agatccgcag acgcgaaccc agggattcgc
      421 acattttgag gatgtcaggt tcgtcgaagt cataagcgaa catatcgacg ctgatcgagt
      481 cgtcgcttac acattccttg agcagatcga agaccatctt gcgggcgtga aatccgagcc
      541 attcgtactg cggctgatag gacgtggtgt cgtagtccag agtcttgggt tggggacgga
      601 tgtccttgtt ctggaactta gcgacataag cctgcgaact gacgtaagcg cgagtgagtc
      661 ccaattcgaa attgccggtc tgcggcttga cgagttgaac ggtaacttcg cccgaagggc
      721 catcgaccaa cgcggtgccc tggcaatagc gcgcagtcac cttgtacttg aagtctccgg
      781 gaacgacgtc gggaggaaag tgtacccagt gaaacttctg gaagggagca tcctgtgatg
      841 aagtccaggg atgctgctgg ggagcggtgg cggaagtcac tggctgagtg aagttaatcc
      901 ggttctgcaa atagaaaggt tgtccggtcg gcggagtgca ctggatggaa aatccggcca
      961 gatcgttttg tttgagcaca ctgtcgtcga gactgaaggc gagcagcaca tcgccatcac
     1021 cggcgtaggc cgcgacggtc aatccattgc tggatacgcg atcgctggga gcggaagaca
     1081 tggcaacctc ctggggaatc cccactgcaa ctgaaaacta cttacgactg gttgaattga
     1141 ccgggcaggg agagaaaata cagccctcaa ctccacgccc attgagaatt cgcagcgccg
     1201 ctgaaatatt agcgcgcgaa ttccgattca tctgcagaaa cggtaatgat tgtaaccccg
     1261 ttttcgcggg acgtgttgct ccattccctt gcaaacaagc tcatatctac gcatttgttt
     1321 gacgacggca caaaagtgga taaactcggc gctgctcttc cgaagctgag ctacatttcc
     1381 gacgattggg cgaaaaccga tccggcattt tggaagccga aggtcgcggc tccgacaact
     1441 aagccagccg cgaagccgga agcaaaaccg gcgcaatagc tggcatcctt aacttgggtg
     1501 gttgcggggg actgcaatcg cccattctcg cgttcagtcg gcgaatcgcc ctggttgccg
     1561 atttccttgg ttctcagcgt cggataaatg gttgcgcggc agtcgccggg gtgcgatagc
     1621 attaaaggag acgcgcaact cagatgactg aaatcgtcac gaacccggac ctatctccgt
     1681 accccgcgcc acgggggtcc aaacccgcat atcacgcgat ggtgaagccc attggcgcga
     1741 tctgcaatat cgactgcacc tactgctact acctgcacaa gaaagagttg ctgggaagcg
     1801 acagcaaatt ccgcatcacc gacgaaattc tcgaagagca catcaagcag tacatcgaag
     1861 gccaggtccg cgacgaagtg gttttttcgt ggcagggcgg agaaccgacg ctgctggggc
     1921 tggagttttt ccaaagagtg gtagaactcg agcagaaata caagaatcca caccagcgga
     1981 ttgagaacga tctgcagacc aacggcacgc tgctgaatga cgagtggggc gcgtttctca
     2041 agcagaatgg gttcctggtc ggactcagca tcgatggccc caaggaactg cacgacgttt
     2101 atcgggtcac tcgtgatggc aagtcgacct tcgataaggt gttcgcggcg gcgcaaatgc
     2161 tgcaccgcca tggtgtgccg ttcaatacgc tgacggtggt gaaccgggtc agtgcgaaga
     2221 agccgctcga tgtctatcgc ttcctgaaga acgaggttcg gccgcggcag atgcaattca
     2281 taccgtgcgt cgagcccaag gtttttcgca atgtggcgcc gcagatgtgg gaccccgaga
     2341 cgctgccgct gtacgattcg ccggccgcac acccgggaaa tgcagactcg gtggtcacgg
     2401 attggtcgat cgatccggat gactggggat attttctctg caaggtgtgg gacgactggt
     2461 atcgccgcga tatggggaaa gcgttcgtta accttttcga aacggcggtc gggcagtgga
     2521 tgggcaagga atcgcaggtc tgcatttatc acgagttctg cggtaagggc gtggccatgg
     2581 aacacgacgg cagcttgtat tcatgcgacc attacgttta tcccgagtac aagctgggca
     2641 acattcagga gacgtcgagt tcgcgcatgg ttttctccga ccggcagaaa gagttcggct
     2701 tcgccaagtt cagcgcgctt ccgaagcgct gccgtgagtg cgagtatcta tttgcctgca
     2761 atggggagtg tccgaagaac cggctgatcc gtaccccgga aggcgagccg gggctgaact
     2821 acctttgcag cgggctgcag aaattctggc gccacatcga cgcagacgtg caggacatct
     2881 gccgccgcat cgcgcgcggt gaacctctca aggctcgttg aacttcggga accaaacagc
     2941 cacaggacat ctccgctcaa gcgaaggaag ttggagcgga gcacccttct gatggtcgat
     3001 ccgcaagcct actcagtccg gggttacgcc ctcgggctgt gggaagtatc acggagattg
     3061 cggagcgctc gaggacgtta tgcgagatat atgatgactt cgccgcagta gcgacgtaga
     3121 ggtgcgaagc gcagaagttg tgcgtcgagt gtcttcatca gctgccagaa tggacgtggg
     3181 cgaaccggca ctcccgggtc agggtaagga agcttggcca gccgcttcaa catgtacaac
     3241 aggttccgct tctcaagccg aatctggctg aagtcacgac agaactcgcg aatattttta
     3301 tcggtaaggg gcgcatctcc agcgtcggca tgttcgctgg tgaagcgccg cgcgagattg
     3361 atcaaggggt tgtcccacag ggtctcatgg aaaatcgcgc ggccgcccgg cgccaaaact
     3421 cggaacagtt gggcgcccgc gcgcggatac ttgatgacat ggtgcagtac cccgaagccg
     3481 atgatgaggt cgaacgatcc gtcagcatag cgaagatctt ccgcatccat ctgttcgaac
     3541 tgagcggtga gtccatagcg gcgtgcgacc tggttagcgg tctcgatggc cgggctggag
     3601 agatcgaagc cccagacctg ggctccgcaa agcgacaagt acataccaag cttgccgaag
     3661 ccgcaaccgt agtccaggac gcgaagtccg cgcacatcgc caaccgagtc cagggcttcc
     3721 ctgcgtagat cagcccacca gttccatccg ccttccgaga aaggcgtgag ttcgacgagc
     3781 tgaaagattc tctggaaatc taccagcgat tccgggtagg ccaccagcgc gtattcattg
     3841 aagaaagcat catgccagtc gcgttcagcc tgcttgatgg caccaccgcc gcgagatgag
     3901 gccgaaaccc cgttttcggg gactggcgac gcgtcgggag aagaggcagg aacatcaggc
     3961 tccgtcgaag acattcgaac ttgagactac cttttcgacc cctccaatgg caaaggtgac
     4021 atgccagttt ggcgggtggg ccggttgtat gcccgcgcgg gccatagcgg tgaggatgaa
     4081 atgagcgatc tacccgtcag attcttactt ggcggtcaag atctccatgg gatcgttgcc
     4141 acttcgctat ctcggatgag ataagccggt agtagtattc gaatgtcgag ccagggagga
     4201 cacgtgccta catccaatgc ggtcatcgat ctctcacaca acaatcagag tgtagatttc
     4261 aacaaggcga aagcggccgg gatattgggc gttgttcaca aggccaccca gggcacgggt
     4321 ttcgtcgacc ccatgtacgc taagcgccgg aaagccgcag ccaacgcagg tttgctctgg
     4381 ggagcctacc acttcggaac cggggccgat ccaatcgcgc aggcgcaaag ttttctaaag
     4441 gtggcaagcc cgaccgccaa agacattctg gtgctcgatt tcgaaccgaa cacgactcct
     4501 ccccgcaata gcatgaccct ggtccaggcg cgggccttta tcggattcgt gaaaaatgcc
     4561 acgggtgtca tgcccggtct ctatggcggc gcttatttgc agcaacaact gggaagcgct
     4621 tccgattcgc ttctcggcgc ctgctggctg tggtgggcgc agtatcgtaa ggcgccggcg
     4681 atcccgccga actggaaggc ttacacacta tggcaataca cagacggtca cagcggcaat
     4741 ccgccgtatg aagtcgatgg gattggaccg tgcgatcgcg atctctatca gggtacgact
     4801 gatgacctgc aaaagaagtg ggtgacggga acactgttgt agcgcgccga gtggctcgat
     4861 gcttgctagt tcttcttttg ccagctccac actgggtcgc ccttattgtc cctgagcgtt
     4921 actttgtcat tcacctttgc cacttctcgt gcgaggacga tgtccgctcc gttctgtttg
     4981 accttcgacc cggtgacggc gatttcctcc cccttgctaa aggtcaggcc catgtcgtcg
     5041 agaaatgatt ttggacaaag gtaaacgtca acgctctcgg tctcattttt caccatcaaa
     5101 tgggcaatct cgctgccctt tggcggcaac tttacctctt cgactgttcc tttcacggta
     5161 gtctcggtgt gcaagtcgta tttgggaacg cctgcgtcgg ccccctgcgc tttttgcgcg
     5221 ctcgccggga gcagtccgga gagtgcaaga aacgcggcca gcaaaacaag cttcttgcaa
     5281 aagttcggca tgctcgactc accggaatca tgcatggctc ttccttccat gaaaccgcca
     5341 gagccgaggc cagtcgtacg caacagctcg tggagatgcg gttcaaggac tagctgtcgc
     5401 ctgggtaggt cgaatccagt ccgtgaccca gaccatcgta ctcctctggc tggcggggta
     5461 cgcgactcgc cggattccgc aacaactctt cagtgataag tggtgagcaa tccgtgtgtt
     5521 tgaatcgctg ccgcagagaa tccccgccca agcagaggaa gcttgaacgg ggaaacctag
     5581 tgtagcgggg agattgaact tttgtcacag caaagcggcg tgcccgtacc acccgctcgt
     5641 tactctatgg cagcgaggtg tcagtagccg ggcaaggctg cggcagcggt caggtctgca
     5701 aaagtgccag ccggtttatc ccccagcggt acgggacagg ccgacgacga tcccaggcaa
     5761 gtcttgacga tgtcgatcag cttccagatg aacatggcac ccttggcgat gacgatgatc
     5821 gccttgatga tgacaccggc ggatgcggcc agggccagca catattgcga ccatcggaga
     5881 gtccggatca cggccagaaa cgccagcccg agcggaccag ccatagcatg catggcactg
     5941 atcagaacgt cgataaactg gcttgccctg aacgttccgg cgatcactcg ttgggcggca
     6001 gcgacgatgt tcaagacggc ttgcctgatt cttggaccgg tcagcagagc ggtggcctgt
     6061 ctttccatct ctgccaccgc cgcccgggga acaaaagcta cgatcagtcc gagcagaaag
     6121 cacagcagct cgatcagtat cttccgcaga cactcgtcgg ataccgcccc aattgccaga
     6181 gccgttgttc ctgtcgaatt cgtggatgtg gcaccggctg cggcgaagtc gacgaagtcg
     6241 ctgaagctat agcactgcag gctggggcac tgagtgtcgt cttccaccga gatgaatggt
     6301 atgtcgacga tgtagttggc aaacgtcgga tcggtgaggg cacgagtcgc ctggtcggcg
     6361 aacatggcct ggatcgacga atcgaggttg tagaacaacg ctccgatgta ggcaagcaga
     6421 atctgctcca gtgctgccgc ctcctgcgag gtgagtggag tgttctgcag ggtagtaccg
     6481 caatccggga taaacggcga cggtggggtt gaaatcaccg gcgaagtggg gttgaccaat
     6541 tcgagtgccg gcggtgaaca cgccccgctg gtgagcgtgc cgccaacagt gacatgctgg
     6601 ccaagcaaac tcggatcgat ggcagcgttc cggagataga tgggaaggaa accggttccg
     6661 cggtccgact cgattacgta gggtggcaca cccgcaaccg aggtcgcgag caagcgcccc
     6721 ccgaccacga tcggaatcgt cgtatcgaca ggggttgcct cgggaagcag ttgtgctagg
     6781 gacatcttcg ctcccgcaag cggggccatc gccatggttg aaattccggc tttcaacaaa
     6841 gtccgacggg agagagaacc agagtttttc atatcgacct cctctcggtc gtggttgcgg
     6901 cggcgattga tttcgataag caaggagcag cttcaccgcg gagctgctcc ttgcaggagt
     6961 cggcttccga actccggcca gacaagccgt ctcctaactt cgccggatca atcgccggcg
     7021 caggtaagtg cccagacctg ctacgcccga accgaacagc agcagacttg cgggttcagg
     7081 gttttgggaa accatgtcga ggttcacgct ctgtacattt cctccgccat ggtcccacag
     7141 gacctgcacg ctcgggcttg tcacgttcat cccgttcttc cacccgagcg cgcctttgac
     7201 tgcaggtggc agcgtcccta cattccaatt cgcgacgaag ttggcgtcgt cggtctgctg
     7261 gcccacggtg aaaaccgggt tggcaatcgt acctgtcatg ttgatcccgt tgcccgagag
     7321 gctgacagtc ttggcagcgg tgtcgaaggt gaagtcgaat tgctttccat tgaacaggtt
     7381 gttctggtcc gtgacgttgc ccgcggtgcc gtcggccttg gtgatcttgt tcaacgcgac
     7441 ggtttcattg acctttccag tgcacttcgt gcaggcgaag gtgacggtct tgacggggtc
     7501 ggcataggcc gcgggcgaga gctgcaaggc agccagggac acgagcgcca gaatgcccaa
     7561 actgaaacga gatgcgatct ttttggtttt catattgacc tccttcaggc cagggttgta
     7621 gcggcgatga cgagtggcgc gtggggcagc ctcaccgcgg ggctgcccca ccgctttcac
     7681 catgcttcgt cgcaggaagt gatgcagtcg ccccggtcgc tggagggttc gcatcgaacg
     7741 tcccggctgt cgcggaggaa gacggctcca taagccggct gcagcaagcg tctccggtcg
     7801 cattcttgga cacgtcccaa aataagggct cggtccctcg actgcgtcca ctgccacccc
     7861 gcgatcgcga cgcataggtg aaataactgg atgaacgaca gggagtggag ctggccatat
     7921 gcgaactctt ggcttggctg attcatcttg tcctcccgac ttccgtcatt gcgagggcga
     7981 cggccgctag cacatgcgcc ctacttgggc ccgggtttag aagcctggag gcaggctcat
     8041 ttcccccatc tgcctccagg tgttctcctc gataaagcgg ttcgtcgaac gtgctcaact
     8101 tttccgctct gggcgtgaac actacgacgg attccgtgct ggattcaatg gttaacgcct
     8161 gacttcagag tggatacggc gtgacttcag gattacctgg aggtgaccgt ctcattcatt
     8221 ggggacaggg gacttaggtg ctactctgac caaacaaagt cggagacctg aagcatcaag
     8281 agcgatataa tccgcaccaa gattgaggca agcattgggg aaatgctttg gccgatacgt
     8341 accgactact tcgcttcggg gtcttcgagt taaacctcga tctggaagaa attcgcaaaa
     8401 acgggacgcc cctgaaactt cccccgcagc cgttcaagtt actggctttg ctggccagtc
     8461 gatccgggca ggtagtgatc cgcgatgaga tccaggagca gctctgggga agcgacacat
     8521 ttatcgattt tgagcagggc gtgaacaagt gcatcaagca aatcaggacg gctcttagcg
     8581 acaacgccga caatcctctt tacctggaga cgattccgcg tcgcggttac cgctttctgg
     8641 ctcccgtcag gaccaagatc attcccgctc cgccgccgca agtgaaggag gccagctcgg
     8701 gcctgctggc aaacgttgtc gcgcaggtgc gcgcacaagt ggagtcggct tccaaaaccg
     8761 ccggcgatgt ttctcagcct gctgccgacg caaccgtgga aggcgcggcg cacgagacct
     8821 ccccgacagc tccagcatcc acgcagagca tgcgaacgcg gctcatctgg gccttggtcc
     8881 cgctggcgat cctgtttgcc gctgtgctgt attggtattt gtcccgtgcg gtgcgatgag
     8941 ttagaaggcc gaaggttgcc ttgctctagc ccgacaacgg aacttggacg gagcaccccg
     9001 ggttgtttgg gatactgaat tcttgtcagc gcgaagagct cgcctcgcca cccgccggtc
     9061 aggacttcga gatgtcgcga acaccttcaa cactgttttc ggtggcccaa gctccaagct
     9121 ccctttgcaa cgattcccta cccgccttca gattagcaag ttcgtattca tagtaatgtg
     9181 agccgccaaa gtcggctggc acgctggcat cccgctgttt gaggatgaag caatccttcc
     9241 cgagcgcgtg tgtcaggccg atctcataaa acacattcgg gttaggcaca gagacctctg
     9301 ccacgaccag ccctgctcgt gcgatggcgc tccagatgtt ctgagtcagg tctcctaccc
     9361 ttgggggtgc gtcgcctcgc acacactgga accgaacagc ttctacggcc ggcctgatca
     9421 tctcttcgaa aaccgaaatc gaccaaggtt cgcgaaacgg catgacgacg aaaacctccg
     9481 gatagtcgac aagcacatct ttcccagacc agcggcgatt cgctaccaag cagcacccaa
     9541 ggattttccg cgacggctca ctaagggaat gcacgcccga ggcgtcaacg agcactgcct
     9601 tgcagtcgat cagcggttga atcgtatcgg ggccgcagtc aacacccgca gacgctagtt
     9661 gccccgctgt cagccccaca accccaggct tcaacgtgta gagaaggtgc agcatcttcc
     9721 agctgtttcg atcgctcaca gcaaacctct ctttgcccct tcgaccagca atcctcgcta
     9781 gtgcgcgagc acggcgggtt gctggcgttc cgttaccatc tgctcgcacc ggacgaacaa
     9841 ctccgccttc gagatttttc ccgcatcggc ctccgctagc agcgccttct gcgcgacgta
     9901 gtccttgtta atgatgcccg ccttgctttg cagcagcctc caggctttgc ggcgctcgac
     9961 gcagaacagc acaagctgcc gcgagatggc gcggtattcc ggtttgccat tctcgccctg
    10021 cagcttgatg tacacgccaa agtcgggaat gaacgagcgg tgttcgggct gcagatagcg
    10081 ccggtacacg acatcctgct gggtgaggcg caacagcatg ttgtcgagtg ggacgagcgt
    10141 cgcggcctgc tcctccttga tcttcttcag gtggttgcgg aagcgggcct cggtggcgca
    10201 ccagtgggcg atggtataac gggcgacttc atcgctgccc ttcgattttg cctcgtacca
    10261 gtcgagatcg agcgagggat tgcccttcag atccagcgct tccggatagg tttctcccag
    10321 ccgcggattg aagacgaact cgggcgagca acgcgagtcg cgcacgcgct gcgcctgggt
    10381 gagcgccatg tcgtcgccca ctccgtgctc gggctggcag gtcgtgaaac attgcaggaa
    10441 cgcggtgccg cgatactcga tcccatcgag gatggcgcgg aacaacttag gcgcgttggc
    10501 aatcgagatt tgggctacaa aaggagagcc gtgccccgcg aggaacgtct cagccaccgt
    10561 cttcttctcg gcgctcttgc cctgcgtggc ctcgccaaac acgttcatgt cgctgccgcc
    10621 aagcatgggc gttgagtcgg agttctgtcc gccggtgttg gagtacacct gggtgtcgag
    10681 catgagcact ttgacgttgg gccggttctg caagaccacc ttcgaggtgt tctggtagcc
    10741 gatgtcgccc atgccgccgt cgccgccaat ggcccagacc ttgggcagtt cgataatttc
    10801 ctggtcggtc attagcagat cgctgaagtg ggtgtaggag tagaagtcga gcggggtcat
    10861 gacttcggtt tcgcgattca gcagcgcgtc cgccaaccgc tcgggtacga ccgagcgccg
    10921 tccgtgatcg acgatgaaac tctcgccaag caaccaaccg atggtgacac cgtcctgaaa
    10981 gagcgagttc atccagggat acgggtgcgg gttgttgggc ggcgtggagc cgtagacggt
    11041 gttgcaaccg gtgtgcgcgg tcatcgccat tacgcacatg ccgttggcga ggcgtccgtc
    11101 gacggcctgc agatcgcgat ggttgaaggc ttcctgccgc atgacggcgc aaatcgcgtt
    11161 gacgatttcc gtatcggaga ttgcgccgtg gttcgccatg cgcgacttgg tatcgcgatc
    11221 atcctcgccg cccagtccca tgagcaagtg ggccacgcac tggcggaata gatcgtattc
    11281 ctcgggatta cgttgcttga gcgcggcgag tttttcgacg cccgtctgct cgagttcgat
    11341 cgctttggtt cgcaggcgat ccgctttggc gtggtagagc ggccgcatgt aggcctcagt
    11401 gcaggcggcg atcgcgcgca gaatgctctt ctcgccgcag cccgcgcagg caccgtcgcc
    11461 gcagaccatc gcgtcgtagt tgtgccgcac catcagcatg ttgcgcaagg tcgcagtctt
    11521 ggaatcctgc ggcttcgcat cgttgtagag tccgaggtat ttctgcggcg tgtcgggcaa
    11581 aagattcagg aacgcggtac cggtttcgtg ttcggcattg accatttcgg tttcgtccac
    11641 catctttagc gcctcgtgct cgccgcaggc ggtgacacac gcagcgcagc ctttgcagag
    11701 gtcggttacg aagatggaga agatgccacc agttcccggc ttcttgcgct ctggagtcga
    11761 gaagatggcg ttgaccttct ggtaggccat gggaactttg tccatgatgg cgaagaactg
    11821 gcgtttggct tcgggcgaga atccgtccac ggcggtcatg acttcgcgga gtatctcggg
    11881 cagcggcgtg tcaactttct tcgcgacgtc gtcggccatc ctctgccgag tacgcttgtc
    11941 gatctcgggg agggagtcca tcatctttct acgttcgcct tgatcggtga cgtagcgcgt
    12001 caccgccgtg ttcaggagcg tttgcaggtc ttgcgcgcag ttgggcagcg ccgtgtcagg
    12061 acacaccgcg atgcactcca tgcattgcgt gcagttttca gggatgaaga gtggcgtctc
    12121 gcggcgcgcg acgtacttgg acgcggtgtc gccggtggag gcggccatca cgccgacgga
    12181 cgcataggcg ttcgatggct ggttgtaacc aaggccggca cggaactggc catcgaagct
    12241 tgcgatctgg gtcaccggag tgcgctcggc ttgcccctca ggagcagggt gggagcggca
    12301 tccgatgccg cacgcgtcgc cgtcctcgct gaaagtagga aaaagagcaa tgccgcgcaa
    12361 actcgagcga tcgagcgcat tcagttcgcc tacgcggatt tcgcgcacct gctcgaagcc
    12421 ctgggtcatg acttccatgt tggactttac gaccgcactg cccagccggc cgaacttctt
    12481 cacgtactga ttgtggacga cttcgagaaa ctgctcgtgg gtgatgcgga actcacctag
    12541 cagcggcgag acccggaaga atgcgccgag gaaagcgttg ccctgcatgc gaagttgcag
    12601 gtcggcgcgg tcggttgcct tgcgggcaat ctggaagccc ggcagggtga agacacggac
    12661 atttttctcg atgatctgct tgcgcgccca caggggcaac tgctcccacg ccagttcacc
    12721 ttcctgatcg gactcccaca ccaggcaacc gccctgggag atgccgtcga gcggattggt
    12781 atgcgtgaag gccttcggat cgcaacacag gaccacggtc acatgacgca agtcgcaatt
    12841 aacgcgaatc cgtttcggcg cagccaccat gaagtaagcg gtgggggccc cctttttctc
    12901 ggagccgtac ttgggattgg cgctgacgtg aataatttcc ttaggattgc ccaactcgtc
    12961 gacgattttg tcgcgctcga agagcagatc attcaagtca ccgatgatcg ctccgaggtt
    13021 cttgccggtg gtgatcgctc cccatccacc gatggaatga aagcgcacgg ccaccgcgcc
    13081 ttcgggcaac agcgatggcg tgtcttcaga cttcacttcg taaggatggt ccacgcccag
    13141 cacgaagaac gatgttccgt cagaggcgtg cttgccgtcc ttgcggggac gggttccggt
    13201 ggcgaattcg taagcgccga agacgtgctc gggacggaag tcgcgcgatc cgcagccgta
    13261 gatgccggag aagagtagcg gcatctgggc aggtgtaatg gcaggcaggc cagcgaccgc
    13321 gggatgtccg tccgtctgca aagctttgtt gagtgcggtg cggatgtcgc ggcccatggg
    13381 attgtcgccc gccatggctt cgtcggtgcg ctccagcacg atgacattct tcttgcccgc
    13441 cagcgcgttc acgaccgcaa cttcggggaa gggacggatg acattcaggt ggatcgagcc
    13501 aaccttcacg ccacggtgct cgcgcaggta atcgaccgca gcctcgatgt tctcggccgc
    13561 cgagcccagc gacacaaata ccgtgtcggc atcgtcggtt ttgtagcacg tcaccaggcc
    13621 gtagtggcgg ccggtgagca tgccgaattc cgcataggcg gcttcgagaa atggcaggat
    13681 gggctcggtg aagttgttgc ggcgcgccac aatcccttgc atgtagtgtt cctggttctg
    13741 caccgggccc agcagaacgg gattggtgag atcgatcatg cgcggcacgc ggcggcgctt
    13801 cggcccaaac aacacgcgct ggctttcggt gggacagtcg atcaggtcgt cgggcgcgcc
    13861 cagaaattcg cggatcaagt ccgcttcatg ccggtagaag gtgcgctcca gatgggaggt
    13921 aaggaatccg tcctggcaat tcatgccggg agtcaggctc aactcggtca cgcgccgcag
    13981 aatcagcgcc tggtcggcgg tctgctgcgc gtccttgccg aacagaatga tccatcccgt
    14041 gtcgagtgcg ccgtaaacgt cgtcgtgtcc gcagtgcacg ttgagggcgt gcttggtcag
    14101 ggcgcgggcc acgacctcca gaaccatggt ggaacacttg ccgggcgcgt ggtaatactg
    14161 ctcgatgccg tagagcacgc cctggcccga cgtgaaattg acgacgcgct tgccgcaaac
    14221 ggaatgagcg atagctccgc cctgggcggc gtgctcgccc tcggtttcga tggccagagt
    14281 gctgccgccg aaaacagtga ggtgtccctc agcgtaggac tgctgataga gttcgccgcc
    14341 ctcggtggag ggcgtgatgg gatagaagac gccagcgtcg gcgatcctgg tttcggtgtg
    14401 ataggaaact agctggttgc cgttggcggt gacccggatt ccgggatagc gaggagctgc
    14461 ggacatggta ccctcccgcg acgccttccc cacagaggtg tcagtattaa attgtgtggt
    14521 gagcaaaatc ttagtcccgc atggggcagc ccgcaaggaa ttgcgtcaca ttcctcgcga
    14581 ggttgtgcaa atttgtgccg ggggccggga agaagctgtc ctttcatgca cttgggtata
    14641 gtatgaccaa atccgggaac cactggccgt gaagacactt tgcggcagga gggaaacatg
    14701 gccgaaaagc aggtttgttt cgtggtaatg ggcttcggga agaagacgga ctacccgacg
    14761 ggacgcgtca tcgatctcga caagtcatat cagtacatca tcaaacccgc cgcggaagaa
    14821 gcggggctgg agtgcattcg agccgatgaa atcatccatt cgggaacaat cgacgttccg
    14881 atgtacgaac aactgctgaa cgcggacgtg gtggtcgccg acgtgtcgac gtcgaactgc
    14941 aacgcctttt acgaattggg agtgcgacac gcgttgcgcc cctataccac cattactatc
    15001 gccgaagata agatggtgtt cccgttcgat gtaaaccaca tcgcaatccg aaagtatcag
    15061 cacctgggcg acggtatcga tttcggcgag gtaatgcgca tgaaggcggc gctgaaggag
    15121 gcgattcaaa cgattgccgc caagccgacg gatgacagcc cggtgtacac attcctgaag
    15181 gacctgcggc ctcccgtgcg aggggcgaaa gaagcggcgc tgaagttcgc gatggcggca
    15241 atatcagatg gaccgtctgc aggccaggac ccggcaaaca ccgcaaccgt cagcactttg
    15301 atggcacagg cgcaagccgc gctggacaag agcgacttcc tgacggcgaa atcgctgctg
    15361 gcggtggtgc gaagcatgat ccccaacgat ccttatgtgg cccagaaatt ggccctggca
    15421 acctacaaga gcaagcttcc agatcccacg caggcattga aagacgctca tgcgatcctg
    15481 actgagctta atcctgaagt ctcaactgac accgagacct tgggactatg gggcgcagta
    15541 cacaagagac tatgggagat aactgccgat cgaagctacc tggatatctc tattttttcc
    15601 tatgagaaag gcttctacct gaagaatgat tactacaatg gaatcaattt agcgtacctc
    15661 tacaacgaac gtgcagccat atccgaagcg cctgagtcgg tcgccgattt cgtgtgcgcg
    15721 gaacgtgcgc gcaagagagt cctgacaatc tgtgactctc tgctgcaatc tgggcgcacc
    15781 ctcccgggcc aggacgagta ttggatcagg gccaccatgg cggaagcgtg gctcggtttg
    15841 ggggacgtga acaaatcgaa agagagcctg gacgcggcgt tcgcgctgca gcccagtcca
    15901 agtcagtgga tgcgcgacag tacgcaagaa cagctgcaga agctcgaaaa gcttctcgct
    15961 tcctcgcctc ttaaaaagac caactgaatc tgcgactggt gg
//
LOCUS       DASRKK010000520         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_238186, whole genome shotgun sequence.
ACCESSION   DASRKK010000520 DASRKK010000000
VERSION     DASRKK010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238186"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1001..1936)
                     /locus_tag="VFC15_11975"
     CDS             complement(1001..1936)
                     /locus_tag="VFC15_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZM10916.1"
                     /translation="MKPDIVRRYAHNPILSKAEIPYPVETVHNAAVVKHGDEYVMLFR
                     SHLRTGRSIIGLARSPDGVHFTADPEPFLTPARDGPFAADEEFGVEDPRVTRLEGEYI
                     ITYSAYSRNGVRVALAKTRDFNQVERVSLITQADHRNVVTFPEKFGGLYARLDRPHSE
                     IAPWSIWISYSPDLHFWGESQLIMRPEQYHWDEMKIGPGAPPIKTDQGWLSIYHGVFR
                     TMDGSVYRLGVALHDLLNPAKVIGVSDSWILQPEDPWEITGYVHNVVFTCGAVAESDG
                     TVKIYWGGADTVMCVGEANVSDLVALCLDHGRPAK"
     gene            complement(1933..2970)
                     /locus_tag="VFC15_11980"
     CDS             complement(1933..2970)
                     /locus_tag="VFC15_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM10917.1"
                     /translation="MTIPNKRVAVLSPVAWRTPPRQYGAWETVASNITEGLVSRGWDV
                     TLFASRDSVTRAHLHAVVDRGYEEDPAIDPKVAEYLHISEAFEHAAEFDLIHSHYDFM
                     ALSYTRLVKTPVLTTIHGFSSPRIMTVYEKYRDGYFVSISNSDRAPGLNYLATVYNGI
                     DLSLYPLQELGGDHLIFLGRIHPDKGVHLAIEVARLSRLPLLIAGIIQDQTYFREQVE
                     PHLDRTIRYVGPVDVPGKNELFAHARALLHLNTIPERFGLVLAEANAAGVPAIAMDLG
                     SCREVIKDGQTGFLVNNVNEAVRALERLSEIDRSACRLRVQRCFSVETMVEAYERVYS
                     TIFDLEGEKRT"
BASE COUNT          652 a          908 c          921 g          634 t
ORIGIN      
        1 gtgagaggga cacgatgtcc gaaaccatcc tggcgcaggc caggcagacc ggggcgcgat
       61 atagactcat ctaaggatgc gacactactc gccgaggtgg gcggggtcgg cgccagcctg
      121 cgatatcgaa actgagcgga aaagggtcag acaaccctgt ccgcggttgg gcgaaggaag
      181 agttcaggcg tttcggcctg aacctcgaat tcctgctgca tgctgaggtc tatgcatcac
      241 atactgtcgg tcgccgttgc gatttccttg cttcctgcag ttcactccgt cgccttcact
      301 ttcagttgtt cttaccacaa cctgctaagg aaagacgccg acaccaaatg cactgagaga
      361 ccatcgccgc gctggcgcgt gaggtgcgcg gcccgccttg gcaccgctca atccccattg
      421 aagccatgcc atttgcacca ggaacgccga aacccgcggg agttccgcga tcaattgatc
      481 gcggccccat tgaagcacgc cgtgccgaaa accagcgcgg cgatggcctc tcaatgcatt
      541 tgctgtcggc ggtgacgtcg gtgtcggcgg ccatctgcgg aggccgagca tatcgaggtg
      601 tcggtgggcg cgatggcccg gttccggacg ttgtgaaacg acagtccctt ctgattccgc
      661 ggcagttatg gccttcgagc tgccccaaag tgcggaagtc atctgggagc attttgctcc
      721 acaccgagca attagcctga tgagttcccg tcagactcgt ggaaccagca actcccccga
      781 aattcgcggt acccgccggt gtccggagtg gtttcgggtc ctgtcggatc caaggcgccg
      841 tttccgaacg ctgtgaaaac gtaagcactt tggagcattc ccgctcaaca gaagcccttt
      901 tctggttcgt tatggccaac tggatgagaa ccagccatcg cgagcgctcg gtcgtggtgt
      961 ccgccatcaa catgaccgac tcgccatccg aacctggagc tcattttgcc ggtctgccgt
     1021 gatccagaca caaagcgacc agatcggaga cattcgcttc gcccacgcac atgaccgtgt
     1081 ctgcgccgcc ccaatagatc ttcacggttc catcggactc cgcaacggcg ccacaggtga
     1141 acacgacgtt gtggacatat cccgtgattt cccagggatc ttccggctgc aaaatccacg
     1201 agtcgctcac tccgatgacc ttcgctgggt tcagtagatc atgaagagcg acccccaagc
     1261 gatagaccga tccgtccata gtccggaaaa cgccgtgata gatggaaagc catccctggt
     1321 ccgttttgat cggcggcgcg cccggaccga tcttcatttc gtcccagtga tattgttcgg
     1381 gccgcatgat gagctgggat tcgccccaaa aatgtagatc cggggaatag gaaatccaaa
     1441 tggaccaagg agcgatttcc gaatggggac ggtcaagccg ggcatagagg ccgccaaact
     1501 tttcggggaa ggtcaccaca ttccgatggt ccgcctgcgt gatgagcgag accctttcga
     1561 cctgattgaa gtccctcgtc ttggcgaggg cgacccggac tccatttcgc gagtaagcgc
     1621 tgtaggtgat gatgtactcc ccttccagcc gggtcacgcg tggatcctcg acgccgaact
     1681 cttcatcggc cgcaaaaggg ccgtcccgcg ccggagtcag aaagggctct ggatcggcgg
     1741 tgaagtggac tccgtcgggg ctgcgtgcca agccaatgat ggaccgtccc gtgcgcagat
     1801 gagagcggaa aagcatgacg tattcgtccc cgtgcttcac caccgccgcg ttgtgaacgg
     1861 tttccactgg gtaggggatc tcggccttgc tgaggatggg attgtgggca tatcgtctga
     1921 cgatgtccgg cttcatgttc gtttctcccc ctccagatca aagatcgtcg agtaaacccg
     1981 ttcgtacgcc tccaccatgg tttcgacaga aaaacatcgc tgaacccgta ggcggcaggc
     2041 gctgcgatcg atttcggaaa gccgctcgag ggctcgtacc gcctcgttga cgttattaac
     2101 cagaaacccc gtttgcccgt ctttgatcac ctcacggcaa gagccaaggt ccatggcgat
     2161 agcgggcaca ccagcggcat tggcttctgc caggacaagt ccgaatcgct ccgggatagt
     2221 gttcaggtgc agcagagctc gggcatgcgc gaacaactca ttcttgcccg gtacgtccac
     2281 cggcccgacg tagcggatcg tccgatcaag gtggggctcc acctgctccc ggaaatatgt
     2341 ctgatcttgg ataatgcccg cgatgagcag cggcaaccta ctcaggcgag caacctcgat
     2401 cgccagatgg acacccttgt cagggtgaat ccggccgagg aagatcaggt gatcaccgcc
     2461 aagttcctgg agcgggtaca gcgagaggtc gattccgtta tagaccgtcg ccaggtaatt
     2521 cagccccggc gcgcgatcgg agttgctgat ggagacaaaa tatccgtcac gatacttctc
     2581 atagacggtc atgattctgg gcgacgagaa tccgtggatg gtagtgagga ccggcgtctt
     2641 caccagccga gtgtaggaca gcgccatgaa gtcgtaatgg ctgtggatga ggtcaaactc
     2701 agcagcgtgc tcgaacgctt cggagatgtg aaggtattcg gctactttgg gatcgatggc
     2761 gggatcctct tcataccccc ggtctaccac ggcatggagg tgagcgcggg tcacagaatc
     2821 cctgctggcg aacaacgtca catcccagcc acgggagacg agtccttcgg tgatgttgct
     2881 ggcgaccgtc tcccacgcgc cgtattgccg gggcggtgtt cgccaagcta cgggcgacag
     2941 gacagccact cttttgttgg ggatggtcat gctggtaaac ggtcttccta attcatggca
     3001 gccaacacct cagcgagtgg gacggttgcg aaacccgtgg cgtgatcggc cagaccatag
     3061 ggaatgatca gttcgccgtt gtggagcaga gcgccgcagg tataaacgac attgg
//
LOCUS       DASRKK010000521         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1412623, whole genome shotgun sequence.
ACCESSION   DASRKK010000521 DASRKK010000000
VERSION     DASRKK010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412623"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1902)
                     /locus_tag="VFC15_11985"
     CDS             complement(481..1902)
                     /locus_tag="VFC15_11985"
                     /inference="COORDINATES: protein motif:HMM:NF033593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HZM10918.1"
                     /translation="MSRLLNPQLSFADLELQRQGIHLDPTLQGIADFLEEHSALVEQV
                     RQDLERGLKKPSAGRHGITPAQILRSLTLMRVKNWDYRELRDRINDGFTLRSFTQFDS
                     SPVPKHDAFNRAFNRLTSATLAVINQSVVQAAVALGLEDGKKLRVDTTVVETNIHWPT
                     DATLLWDTVRTVTRLVEDLHGKLPQGVTGFTNRTRSARRRMQAIQRMTARQREQQQTP
                     KYRELIRITEQVVQNAEQVVAAAQEISGLDLVAGLAIDALRKQITTFCELGDRVIDQG
                     RRRVLQGETVPTEEKIYSIFEPHTDLIKRGKTLKPVEFGHKVFLAESAQGLITNYQIL
                     DGNPVDSIQVQASLDRHKEIFHHVPECYAGDRGFYSTDNVQHCQQAGVGQVCIPQRGG
                     QRTEEQEALERSPAFKKGQRFRAGIEGRISVLFRGRGMKRCLFKGLQRFEILVGAAVL
                     ANNLMRIAAMLEQRKGKRRRAAA"
     gene            complement(1899..2234)
                     /locus_tag="VFC15_11990"
     CDS             complement(1899..2234)
                     /locus_tag="VFC15_11990"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM10919.1"
                     /translation="MKQTTIIGRRTKLAAALPSTTEILRGSLLHRYIRHRTGCAVCAD
                     GKGHSVWVLTVSYPGGRTKQFSLRPEQKPMVQQWLRNYQKLKDTLERICELNHELLRS
                     EKTQSEKKK"
BASE COUNT          501 a          730 c          777 g          598 t
ORIGIN      
        1 tagttaggta gattgacgac gacccgcctg cagacgtgat ggaataagtg tggtgcggac
       61 gtcgacaacg ctcgaaattc acggggcggc agccggataa atcctgtgtc atgagaaact
      121 ccttgccggt cgtacaggtg cccgaactcg ctttgacagg gaattccttt ccataacgac
      181 tttggtcacg aatggggatc gtgagcgttg ccatgcggcg gaatgctacc cccaaaccga
      241 cccggcggct tggctgttag ttagtttctg tcgcgaaatg gatgaaaccg gctattggcg
      301 gccatacggc gcgccttctt ggagcgagga ttttactcgc cgtgtctttg tgcgatggat
      361 ttggtcgggt gtcctcagtc tttctgtaat agtttcataa ttacgggtct gttactacga
      421 aatatgcgcg agtcccgaag actgcatttc tttggccgca gcagactcct ttccgcagcc
      481 ctatgccgcg gctcgccgcc gcttcccttt gcgctgctcc aacatcgccg caatccgcat
      541 caggttgttc gccagcaccg ctgcgcccac cagaatctcg aaccgctgga ggcctttgaa
      601 cagacaacgt ttcatgcccc ggccgcgaaa cagtaccgag atgcgcccct cgatccccgc
      661 ccggaaccgt tgacccttct tgaatgctgg gctgcgctcc aatgcttctt gttcctcagt
      721 tctctgaccg cccctctgcg gtatgcacac ctgaccgact cccgcttgct ggcagtgctg
      781 cacattgtcg gtgctataga atccgcggtc gccggcgtag cattccggaa cgtggtggaa
      841 gatttccttg tgacgatcca aggaagcttg gacctgtatg ctgtccacag gattcccatc
      901 cagaatctgg tagttcgtga tcaagccctg cgcgctctcg gccaggaaaa ccttgtggcc
      961 gaattccacc ggcttcagcg tcttgccccg cttgatcaga tcggtgtggg gttcgaagat
     1021 cgaatagatc ttctcttcag tagggaccgt ctctccttgc aatacccggc ggcggccctg
     1081 gtcgatgaca cggtctccca gttcgcagaa ggtggtgatc tgcttgcgga gcgcgtcgat
     1141 cgccaagccc gcaaccaaat cgagcccact gatttcttgc gcggcggcga ctacttgctc
     1201 tgcattttgc accacctgct cggtgatccg gatgagttcg cggtacttgg gggtttgctg
     1261 ctgctcccgc tgccgggcag tcatgcgctg gatggcttgc atgcggcggc gagcgctgcg
     1321 cgtgcgattg gtaaagcccg tcactccctg gggcaatttc ccatggagat cttcgactag
     1381 acgcgtgacc gtgcgcaccg tgtcccacaa taaggtggca tcggtgggcc aatggatatt
     1441 ggtttccacc accgtggtgt ccacacggag tttcttgccg tcttccagtc cgagtgccac
     1501 ggccgcctgc accacggatt ggttgatgac ggccagcgtg gccgaggtga gccggttgaa
     1561 ggcacggttg aacgcatcgt gtttggggac agggctgctg tcgaactgcg taaagctgcg
     1621 cagggtgaag ccatcgttga tgcggtcgcg cagttcgcgg tagtcccagt tcttgacgcg
     1681 catcagagtc aacgagcgca ggatctgcgc gggggtgatg ccgtggcgtc cggcgctggg
     1741 tttcttgaga ccgcgttcca gatcctggcg cacctgctcc acaagagcgg aatgctcctc
     1801 cagaaagtcg gcgatgcctt gcaacgtggg gtcgagatga atgccctgcc tctgcaattc
     1861 gagatcggca aagctgagtt gtggattgag gagacggctc atttcttctt ctcggattgg
     1921 gtcttctcgg atctcagtag ttcatggttg agttcacaga tccgctccag agtatccttg
     1981 agtttttggt agttgcgcaa ccactgctgg accatgggtt tctgttcggg acggagactg
     2041 aactgtttgg tgcgtccacc tggataactg acggtcaata cccaaactga gtggcccttt
     2101 ccgtcggcgc atacggcaca accggtgcga tggcggatgt agcggtggag caaagaaccg
     2161 cgaaggatct cggtggtgga tggcagagcg gcggccagct ttgtgcgccg tccgatgatc
     2221 gttgtttgct tcatctatct aaatgaaaca acaaggagag gaatctgtca agcgctttcg
     2281 aacattattg ttcagatttg ttttgatcca cgccgtcacc atcgccgcag agtcgagtgg
     2341 aactgttacg agaaccgcgg ccggctgaaa tcacggacgc ctccgggacc gcccatccgc
     2401 caattgtggc cagccaaaag ctgcctaact cactccaaat cagcgggtta aggatttcgc
     2461 gacagaaact agctagaggg cggtggcgga gttggacggg gagacgaagc tgaggggaag
     2521 aagaaggcca gggccggggc agctgccgat agcaacaggg atagctcatc gcccttcgcc
     2581 gccttaagag cggcaagcgt cactac
//
LOCUS       DASRKK010000522         9126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_825639, whole genome shotgun sequence.
ACCESSION   DASRKK010000522 DASRKK010000000
VERSION     DASRKK010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825639"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2851)
                     /locus_tag="VFC15_11995"
     CDS             complement(212..2851)
                     /locus_tag="VFC15_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZM10920.1"
                     /translation="MLALVASSAHAQQQAAGQTLPYMNPALPVDQRVKDLIGRMTLEE
                     KVSQMRDHSPAIPRLGVPKYDWWNEGLHGVAFAGYATNFPQVIGMAATWDTDLVHVMA
                     QTTSTEARAKYNQAMRDDDHEMFFGLTFWAPNVNIFRDPRWGRGQETYGEDPFLTGQM
                     AVAFVSGMQGDDPKYLRVVSTPKHYAVHSGPEPLRHGFNVDVSPHDLEDTYLPAFRAA
                     VTGAHAQSVMCAYNAIDGAPACANTMLLQDHLRDAWHFDGYVVSDCAAVADVYTGHHY
                     APDMARAAAAAVKAGTDLECGYGKGQAYPALVQAVQQKLITEAELDIALTRLFRARFE
                     LGMFDPPSSYTYGRIPFSEVNSPEHRQLSLKAARESMVLLKNADHALPLKAGIASIAV
                     VGPTAELVQSLQGNYNGPPPSPVFPLAGIEKRFPSARIAYAQGSSLVEGLAMPIEHTA
                     LHPASGPGYGLTGEYFNNKEFNGTPVLTRTDRSINFNWDKVVPVAGLQRNNYSVRWSG
                     TFTPPGPAEYKLGVRINYCYACENAEGFRLYLDGKLLVASNEKATPERGAVSDEAVHF
                     KDTQPHQIRLEYIHGTGSAGIDLTWQAPAAVLGDEAVKAARQSDVIIATVGLSPSLEG
                     EEMPVKLEGFSGGDRTSIDLPKVQEDLLKALAATGKPLVVVLQNGGALAVNWAQEHAN
                     AILEAWYPGEEGGTAIAETLAGDNNPAGRLPLTFYSSLSQVPKFEDYSMSARTYRYFG
                     GKPLYGFGYGLSYTTFAYSNVKVSSQLKAGEPLTVTGDVKNTGSVAGDEVVELYLSQP
                     KQFQTPIRVLASFQRIHLEAGQSAHVNLTVDPRSLGQVDAKGNRVVVPGDYVVSLGGS
                     QPGESASIQTATFKVTGKAELPK"
     gene            3384..5981
                     /locus_tag="VFC15_12000"
     CDS             3384..5981
                     /locus_tag="VFC15_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HZM10921.1"
                     /translation="MQNTPPPSPTRTALCSRFIIFIRGLDKVLQRTILLFVAFLLLTC
                     PGFSESGGDAWLRYARLDPVVASRYASLQAAVVTLDHSAVLQSAQMELLRGVRGMLGR
                     TLRIEDSAMAEAAIILGTVEQIRTLDAGFHPPSDLKDDAYALISTRIKGQNALVITGA
                     TERAVLYGAFALLRKIAEGESTSALNEVQQPSAPVRWVNQWDDLDGSIERGYAGRSIF
                     FENRSVRPDLTRATEYARLLASVGINGCNVSNVNADLHILHLGFIPQLARIADVFRPY
                     GVQLGVAVNISMPKQVGGLDTFDPLDQKVAGWWKEKFDEVYRQIPDFGGVVVKADSEG
                     QLGPSVYGRNAAEAANVIAKALRPHGGIVFYRAFVYDHHLDWNNLKADRARAAYDIFH
                     PLDGQFDDNVIVQIKYGPIDFQVREPASPLFGGLVRTNQAIELQITQEYTGQQRHTVF
                     LVPMWKEVLDFDMAAGGQRTPVKDIVAGRSFHRPLEGYAGVANVGLSTDWLANHLAMA
                     NLYGFGRLAWNPDVSSQTIAEEWTRLTFGNDPEVVKTVTNILLDSWPAYESYTGPLGL
                     QTLTNITGPHYGPAPESQERNGWGQWIRADHDGVGMNRTVATGTGFVGQYSPSVQKIY
                     EPLANTPDNIVLFFHHVPYTYQLHSGKTVIQTIYDSHYEGAEKAADFVEKWESLKGHV
                     DDERYEAVLAQLKYQSGHAIVWRDAVDDWIHQMSGIDDQDGRVGHHSNRVEAETMELQ
                     GYKIFEPVSWEGASGGKGVVCVPEAATCSAQTKFTGAAGWYEIDVEYFDLSNGESKFR
                     VSVNDQAVDEWRADMLLPGKDPSADSSVRHRTRGLALRPGDVIRIEGTPDGAERAPLD
                     YMEVLPLEH"
     gene            6520..7419
                     /locus_tag="VFC15_12005"
     CDS             6520..7419
                     /locus_tag="VFC15_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZM10922.1"
                     /translation="MAPLSDLTITIHLETVPADQTGHPGSRSTSYLVHGDLVQAVELQ
                     GAKQIEHWYFIAGIDVLAPSSAVSTVALGDSITDGHGATTNGNDRWPDLLARRLQAVP
                     QGRTIAVLNQGIGGNRLLLDGLGPNALARFNDDVIAQAGVRSLIVLEGINDIGMLGRS
                     GEATPAEHEQLVNDILGAYQQIIERARTNGIRVAGCTILPFMGSSFYLPGPATEADRQ
                     MINKWIRTPGHFDAVIDFDQVMRDPANPDHLLAKFDSGDHLHPSPSGYAAMANAVPLA
                     FLMPAEVPGPKQPASARSAASHR"
     gene            complement(7885..>9126)
                     /locus_tag="VFC15_12010"
     CDS             complement(7885..>9126)
                     /locus_tag="VFC15_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HZM10923.1"
                     /translation="EVEMHRPDGVPDTVQQVKHGALLILAQFHNIGHAIRGTHEPDLR
                     QYTQLGDGASKTDGRIYDPKLGPNEVKGDYSGKPDDRWIFSTTNPYFQWNAIAALAAA
                     SDVLKGWDDALAKDCLETAIHAWKDEKAHPTPSQAGRGFDDAAPTGDPGVGVLAASQG
                     VVSGAQGGRNGRGSSVAPTGSGTTSAPTVPPAGFGRRGSGIGQDWAAALELTIATNGA
                     EPYKSRLKELFPQVITPQQMELRGWMAVRALPYLDAGAKDQMREAVKTYMAGLGKELD
                     ATPFGVPPSMGTWGGSGAVVDMAIRMYFLHKAFPDLVSSEYTLRAVNYILGTHPVSST
                     SYVAGVGTVSKTMTYSNNRADNAYIPGAVIPGYIIIKPDFPECIDDFGFLWFEDEAVV
                     AGSASWVVAGNAADAITKESK"
BASE COUNT         1853 a         2632 c         2712 g         1929 t
ORIGIN      
        1 cggccgattc cgctcacgtc aagtggcaga cggaaggaag ctcctactgg gcatgttgcg
       61 accagatcgt gtcgggggaa ggcaaggcta gccgctacta tccgcgcatc attaccagcg
      121 cgctttggat ggattaggtc ctcacgtgag agggagaaca acagggcact ccccgtttgc
      181 cagggagtgc cctgtttatt ttccgcatga tctacttggg aagctcggcc tttcccgtga
      241 ccttaaacgt cgcggtttgg atcgacgccg actcacctgg ttgcgaacca cctaacgaca
      301 cgacgtagtc tccggggaca accacacggt tccccttcgc atcgacctgc cccagcgagc
      361 gcggatcaac cgtcaggttg acgtgtgccg actggcccgc ctccaggtgg attcgctgga
      421 acgatgccag cactcggatc ggcgtctgga actgtttcgg ctgactgaga taaagttcga
      481 cgacctcatc tcccgccacg ctcccggtat tcttcacatc cccagtcacg gtaagcggtt
      541 cacctgcctt tagctgcgat gaaactttga cattgctgta ggcaaatgtc gtgtagctga
      601 gtccgtatcc gaaaccatag agcggcttgc cgccaaaata ccggtacgtg cgcgcactca
      661 tcgagtagtc ctcaaacttt ggcacctggc tgagggacga atagaacgtc aatggaagcc
      721 gccccgctgg attattgtcg ccagccaacg tctcggcgat cgccgttcca ccctcttcgc
      781 ccggatacca ggcttcgagg atcgcgttcg catgctcctg cgcccaattc accgccagcg
      841 cgccgccgtt ctgcagcaca acgaccagcg gcttgcccgt agcggccagc gccttcagca
      901 gatcttcctg cactttcggc aaatcgatgc tggtccgatc gcctccgctg aagccctcca
      961 gcttcaccgg catctcctcg ccttcgaggg aaggtgaaag acctaccgtc gcgatgatta
     1021 catccgactg ccgcgcagct ttcactgctt catcccccaa caccgccgcc ggtgcctgcc
     1081 aggtaaggtc gatgccggcg ctgccagtgc cgtgaatgta ctccaggcga atttgatgtg
     1141 gctgcgtgtc tttgaagtgg accgcttcat cgctcaccgc acccctttca ggagtggctt
     1201 tctcattgct ggcgacgagc agtttgccgt ccaggtaaag ccggaacccc tccgcgtttt
     1261 cgcaggcgta gcagtagttg attcgaactc cgagcttgta ctctgcgggc cccggcggag
     1321 tgaaggtgcc actccatcgc acggagtagt tattgcgctg caggcccgca accggaacga
     1381 ccttgtccca gttgaagttg atgctgcggt cggtgcgtgt aagcaccggt gtgccgttga
     1441 actctttgtt attgaagtat tcacccgtca gtccgtagcc agggccgctc gcagggtgca
     1501 atgccgtgtg ctcaatcggc atcgctaaac cttctaccag actcgacccc tgggcatacg
     1561 caatgcgggc cgacggaaag cgcttttcga ttccagccag cgggaagact ggcgacggtg
     1621 gaggcccgtt gtaattgccc tgcagtgact gcaccagttc ggccgtcgga ccgaccacgg
     1681 cgatgctcgc aataccggct ttcaagggaa gcgcatggtc ggcattcttc agcagaacca
     1741 tcgactcccg cgctgcctta agcgaaagct gccgatgctc aggtgaattg acctcgctga
     1801 acggaatccg accgtaggtg taacttgacg ggggatcgaa catcccgagt tcgaaacgcg
     1861 cgcgaaaaag gcgcgtgagc gcgatgtcga gctcagcttc ggtgatcaac ttctgctgca
     1921 ccgcctgcac cagcgcagga taagcctggc ctttaccgta tccgcattcc agatcggtcc
     1981 cggccttgac cgcggccgct gccgcgcggg ccatatcggg cgcgtagtga tgaccggtgt
     2041 acacgtcggc gacggcggcg caatcgctga ccacgtagcc atcgaagtgc catgcatcgc
     2101 gcaggtggtc ctgcagaagc atggtgttgg cgcacgccgg agcaccgtca atcgcgttat
     2161 aggcgcacat taccgactgg gcgtgcgctc cagttacggc cgcacggaac gctggcaaat
     2221 acgtgtcttc cagatcatgc ggcgagacat cgacattgaa tccgtggcgc aggggctcgg
     2281 ggccgctgtg taccgcatag tgcttcggag tggaaaccac tcttaaatat ttcggatcgt
     2341 cgccctgcat tccggagaca aaggcaaccg ccatctgtcc ggtaagaaac gggtcctcgc
     2401 cataggtctc ctggccacgc ccccaccggg gatcgcggaa gatattgacg ttcggcgccc
     2461 agaacgtgag gccgaaaaac atctcgtggt catcgtcgcg catggcctgg ttgtacttgg
     2521 cgcgcgcctc agtcgaggtc gtctgcgcca tcacatgcac gaggtccgtg tcccaggtcg
     2581 ccgccattcc aatcacctgc gggaagttcg tggcataccc ggcaaatgcg actccatgca
     2641 gaccctcgtt ccaccagtca tacttgggta ccccgaggcg aggaatggcc ggcgagtgat
     2701 cccgcatttg cgacactttt tcttccaggg tcatgcggcc gatcaggtcc ttcacgcgct
     2761 ggtctaccgg cagcgccgga ttcatgtaag gcagcgtttg gccggcagcc tgttgttggg
     2821 catgcgcgct agacgcgaca agtgcgagca acgaaaagca aaagaccacc ctgaacaagg
     2881 cacaggtacg tgggtatcgc attaagaaac cactcctctc gagaacgtct tagaaaacgc
     2941 gcattatacg gatcagggtg agtgcctgta catcccggca cgaatccgct gcgaaacggg
     3001 atgacctgct ccccagaata atctcctgca agccggcttc gggccaatgt gacaggcgaa
     3061 tgctgctcga cgcattaggc tcagttcagt taggggtacg acatgttctc aacattaata
     3121 tgaacacgcc actgctacgg ccttgtagga cttgatttaa gctggtttgt ccttaccatt
     3181 acatgcaaga agaggcaatc gacatcatga cgccgccact cgaagcgaat gggctatggt
     3241 atgccagccg gcactggatc aacgtcatcc cgggtatcgt cacgtttacg gcggagtcgt
     3301 tcaactgcat cggtgcaaac aaggtctttt caattatgcc tattccgcca gtcctgccat
     3361 ggcggcaaac atggacaaca taagtgcaaa ataccccgcc accttcaccg acgcggacgg
     3421 cgctgtgttc gcgctttatt atcttcatcc gcggccttga taaggtactg cagcgcacta
     3481 ttctgctatt cgtagctttc ttacttctta cctgccccgg gttctcggag agcggtggtg
     3541 atgcgtggct tcgctatgcg cgacttgatc ccgtcgtggc cagtcgatat gcgtcgcttc
     3601 aggctgcggt ggttaccctc gatcactcgg cggtgttgca atccgcgcaa atggaactgc
     3661 tgcgcggagt tcgaggaatg ctggggcgca cgttacgtat cgaagacagt gcaatggcgg
     3721 aagccgccat tatcctagga acggtggagc agatccgcac cctcgatgca ggatttcatc
     3781 cgcccagcga tttaaaggat gacgcctatg cgctgatttc cacgcggatc aagggacaga
     3841 acgcacttgt tatcacgggt gcaaccgagc gtgccgttct ctatggggca tttgcgttgc
     3901 tgagaaagat cgcggaggga gaaagtactt ctgctctcaa cgaagtgcaa cagccgtcgg
     3961 caccggtgcg ctgggtcaac cagtgggacg acctcgatgg ctcgatcgag cggggctacg
     4021 ctggacggtc gatcttcttc gagaaccgca gcgtacgccc ggacctgacg cgtgcgacgg
     4081 agtacgcgag attgctggcg tccgtcggta tcaatgggtg caacgtcagt aacgtgaacg
     4141 ctgacctcca cattctccac ctgggtttca tcccacagct tgcacgtatc gccgatgtgt
     4201 ttcgaccgta cggcgtgcag ttgggggtag cggtgaacat cagcatgcca aaacaggtgg
     4261 gtggcctgga caccttcgat ccgctcgatc agaaggtggc tggctggtgg aaagagaaat
     4321 tcgacgaggt ctaccggcag attcctgatt tcggcggcgt ggttgtgaaa gcagattccg
     4381 agggacagct gggaccgtcg gtttatgggc gcaatgccgc ggaagcagcc aatgtgattg
     4441 cgaaggcgct gcggccgcat ggagggatcg tcttctatcg cgcgtttgtg tacgaccatc
     4501 acctggactg gaacaacctg aaagccgacc gcgcgcgcgc cgcctacgat attttccatc
     4561 cactggacgg acagttcgac gacaacgtga tcgtgcagat caagtacggc cccattgatt
     4621 ttcaggtgcg cgagccggcg tcaccgttgt ttgggggact cgtaaggacg aatcaagcta
     4681 tcgaactgca gatcacccag gaatacaccg gccagcagcg ccatacggtc ttcttagtgc
     4741 cgatgtggaa ggaagttctt gacttcgaca tggcagcggg tggacagcgc acgccggtga
     4801 aagacatcgt ggcgggacgg agttttcatc ggccgctgga aggatacgcc ggggttgcga
     4861 acgttgggct gagtacggac tggttggcaa atcacctagc catggcaaat ttgtatggat
     4921 tcggccggct ggcttggaat ccggacgtaa gttcacaaac aattgccgag gaatggacgc
     4981 ggctgacgtt tggcaatgat cctgaagtgg taaaaacggt gacgaatatt ctgctggact
     5041 cgtggcccgc gtacgagagc tatacaggtc cgctcgggtt gcagacgctg accaacatca
     5101 cgggaccgca ttatgggccg gcgccggaat ctcaggagcg caacggttgg ggccagtgga
     5161 tccgcgcaga ccatgacgga gtcggaatga accgcactgt ggcgacagga acaggatttg
     5221 tcggacagta ttcgccgagc gtccagaaga tctatgaacc gctggccaat acaccggata
     5281 acatcgttct gttttttcat catgtgccgt acacctacca gctgcactca ggcaagaccg
     5341 tcattcagac aatctacgac tctcactacg aaggcgccga gaaggcggcc gatttcgtgg
     5401 agaaatggga gtcgctgaaa gggcacgtcg atgacgagcg ctatgaagct gtgctggcgc
     5461 aattgaaata tcagtcggga cacgcgatcg tttggcgcga tgcggttgat gactggatcc
     5521 atcagatgtc tggaatcgat gaccaggacg gtagggtggg acaccattcg aatcgcgtgg
     5581 aagcggagac gatggagttg cagggttata agatctttga accggtgtcg tgggaaggcg
     5641 cttcgggcgg aaagggcgtg gtttgcgtgc ctgaggcggc aacgtgttcc gcgcaaacca
     5701 agttcactgg agctgccggc tggtacgaga tcgatgtcga atacttcgac ctgagtaacg
     5761 gggaatcgaa gttcagagtg tcggtgaacg atcaggcggt ggacgaatgg cgcgccgaca
     5821 tgctgctgcc gggcaaagat ccgagcgcgg attcctcggt ccgacatcgc actagagggt
     5881 tggctctgcg tccaggtgat gtaattcgta tcgaagggac gcccgatggt gccgaacgtg
     5941 cgcctctgga ctacatggaa gtgttgccgc tggagcactg agagaggttg attcccaatg
     6001 aactgacagg atgcctggtt gtagtcggag tggtggtggc agtacgcaac ctatatcgat
     6061 gcggtaaccg cgggggaatg gtggagtatc cgccgcggat actcccgaat ctagatgttg
     6121 atgagacttg caagaagtat tcgacgactg gtcgttgttt ccgtcatcct tagtgctgtc
     6181 ggcatagccg tccaaccgca acagaactgg gtcggttcct gggcagcatc gcagcaactg
     6241 gttgagcccg ggaattccat ttctgcggaa gatcttaccg acgcgactct gcgacagatc
     6301 gttcatctca caattggtgg tggggagctg cggttgcacc tgtccaaccg atacggttcg
     6361 gctccgctgc atatcgcgtc cgtgcacatt gctcggccag tatctcccga ttcaagcaag
     6421 atcagcccgg gttcggaccg ggctctagct ttctccgggc gccggagtgt caccattcct
     6481 gcaggagcag actccctttc cgatccggtg aagtattcga tggcgccttt gtctgatctg
     6541 accattacaa tccatcttga gacagtgccg gccgaccaga ctgggcaccc cggctcgcgc
     6601 tcaacttcgt acctggttca cggagatctg gtacaggctg tcgaactgca gggcgccaaa
     6661 caaattgagc attggtattt cattgcggga atcgatgttt tggcgccttc ctccgcggta
     6721 tcgacggtcg cgttggggga ttcaatcact gacgggcacg gcgccacaac caacgggaac
     6781 gatcgctggc cagaccttct tgcccggaga ttgcaggcgg tcccgcaggg tcgaacgatc
     6841 gcggtactga atcaggggat tggcggtaat cgccttttgc tggacggcct gggacccaat
     6901 gcgttggcgc gctttaacga cgacgtaata gcgcaggccg gagtacgttc cttgatcgtt
     6961 ctggagggta ttaatgatat cggtatgttg ggccgaagcg gagaggccac gccggccgaa
     7021 cacgagcaac tggtcaatga catcctcggc gcgtaccagc agatcatcga gcgtgctcgc
     7081 accaacggca taagggtggc aggctgtacg atcttgccgt tcatgggctc gagtttttat
     7141 cttccgggac cggcaactga ggccgaccgc cagatgatca acaagtggat tcggacaccg
     7201 ggccactttg atgcggtgat cgatttcgac caagttatgc gcgatccagc aaatcccgat
     7261 cacctcctgg cgaagttcga ttccggcgac cacttgcatc cttctccttc cggatacgcc
     7321 gcaatggcca atgcggttcc actcgccttc ctgatgccgg ccgaagttcc ggggccaaag
     7381 caaccggctt ctgctcgaag tgccgcgagt catcggtgag aaatgaacga agagcttcta
     7441 tgttttttgt ggtcgccact tactcgggca gtaagtgtgc gatggcgtca ctgacagcga
     7501 gtttgtatcc gccgctcaca ttctagatat ctgatttggc ggttgagcta cgcagcgtgt
     7561 aagcaagtat cggaagcgta ccggcacaag ggtgccgccg gaccgggtgt gatgcggttc
     7621 gccggtacgc ttttgattca gccctgggac ttgtcgttag ctatcaatgt ttcctccgag
     7681 cgaggccata tggcttcggc gcacgtgcaa cgacccgcga agcatgcgcc tgctgcaggt
     7741 cgcgcgtaca tgcgcccaga atccgtcccc ctgtatgtcg cccttctcgt agatgcaatt
     7801 gaaggccgcg ccgcaaagga tttctccaaa acaagaaaac gctcggcacc cctcgggggc
     7861 gccggacgct tctagtctgt ggcgctactt cgattccttg gtgatcgcgt ccgcagcgtt
     7921 gccggcaacg acccaacttg ccgacccggc cacgacggct tcatcctcga accaaaggaa
     7981 tccgaagtcg tcgatgcact ccgggaaatc cggcttgatg atgatgtatc ccggaatgac
     8041 ggcacccgga atgtaagcgt tgtcggcgcg gttgttgctg tacgtcatcg tcttcgagac
     8101 cgtaccgacg ccggctacat acgaagtgct ggatacgggg tgcgtgccga gaatgtaatt
     8161 gacggcgcga agtgtatatt cggagctcac aagatccgga aacgccttgt gcaggaagta
     8221 catccggatg gccatgtcaa ccaccgcgcc cgatccgccc catgtgccca tgctcggggg
     8281 tacgccgaac ggggtggcgt ccagttcctt gcccagaccc gccatgtagg tcttcaccgc
     8341 ttcgcgcatt tgatctttcg cgcccgcgtc caggtaaggc aatgcccgga ctgccatcca
     8401 accacgcagt tccatctgct gcggcgtgat cacttgcggg aacaactcct tcagacggga
     8461 cttgtagggc tcggcgccat ttgtggcaat cgtcaattcc agcgccgcag cccagtcctg
     8521 tccaatgcca gaaccgcgac ggccgaagcc ggctggagga acggttggtg cgctagtcgt
     8581 gccagaacct gtcggcgcga ccgagctgcc ccgtccgtta cgaccgcctt gagcgcccga
     8641 caccacgccc tgagatgcag ccagcacgcc gactccgggg tcacctgttg gagcagcatc
     8701 gtcgaagccg cgccccgcct gactcggcgt cgggtgagcc ttctcgtctt tccatgcatg
     8761 gatcgcggtc tcgaggcagt ctttcgctaa tgcatcgtcc cagcctttca gcacatcgga
     8821 cgctgccgcc agcgctgcaa tcgcgttcca ttggaaatac gggttcgtgg tggagaaaat
     8881 ccagcggtcg tccggcttgc cggagtagtc gcccttcact tcgttcggac caagcttcgg
     8941 gtcatagatg cggccgtccg ttttcgacgc tccatccccc agctgcgtgt attgccgcag
     9001 gtctggttcg tgagtgccgc ggatggcgtg gccgatattg tggaactgcg ccaggatgag
     9061 cagtgcgccg tgcttgacct gctgcaccgt atcgggcacg ccatcgggac ggtgcatctc
     9121 aacctc
//
LOCUS       DASRKK010000523         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_825864, whole genome shotgun sequence.
ACCESSION   DASRKK010000523 DASRKK010000000
VERSION     DASRKK010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825864"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..439
                     /locus_tag="VFC15_12015"
     CDS             224..439
                     /locus_tag="VFC15_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HZM10924.1"
                     /translation="MTADRSAPAVDDADLVNGPEDVLVDWDEVEWRRVEQDVRRLRQR
                     IFTASQAGDLKRVRNPQSAKADAPVPR"
     gene            904..1506
                     /locus_tag="VFC15_12020"
     CDS             904..1506
                     /locus_tag="VFC15_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZM10925.1"
                     /translation="MANALETAEEVLESRLRDYRGSLDLLAGHEALKAAAEAAELIWD
                     VICSGGKTIFFGNGGSSMDAGHLAAELLGRFYFERPAIAALSLADVTAAVTAIGNDYG
                     YEYAFSRQLEGVGHVNDVAIGLSTSGNSPNVVNAFAVAREKGLRTVALTGSGGGRVAA
                     MADVLVAVPSSDTPRVQEMCMHIGHSICEYVEARWLASAL"
     gene            1485..2039
                     /locus_tag="VFC15_12025"
     CDS             1485..2039
                     /locus_tag="VFC15_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZM10926.1"
                     /translation="MAGECPVAGVCVAFLDRDGTINVKKPEGQYVLAPQEVNLVPGAG
                     RAVRELNDAGWRVIVVTNQRALARELMSNGDLARVHARLDQLLAGFGARVDEYRVCPH
                     REGTCWCRKPHPGLLKEYMSAHADVDVRKSWMVGDSESDVEAGMRASVRTLRLAGGAT
                     ATRAERVEPDLSHAVKFMLGVSGS"
BASE COUNT          416 a          604 c          726 g          476 t
ORIGIN      
        1 agatcgaggc gacccccttc ggggttgaaa tctgtccgcg tcactttcca cgggtcctca
       61 agtcccaacg ccatcttgaa cagatcgaga ttgcctgacg tcatgtcgcc cctcttcgtc
      121 tgaccactga gtgtcatgac cgtatgggca actagggccg atcaggtgga cctcaggggt
      181 ccaaatccac acgatctagc gaggagccga caagttggac accatgacgg cggatcgatc
      241 ggcgcctgcc gtcgacgacg ccgacctggt gaacggaccc gaggacgttc tcgttgactg
      301 ggacgaggtt gagtggcgtc gagtcgaaca ggacgtacgg cgattgcgtc aacggatctt
      361 cacggcgtcg caggctgggg acctcaaacg ggtccgcaat ccgcaatctg caaaagctga
      421 tgctccggtc ccgcgctaac acgctggtca gcgtgccggg cagcggactc ggttcttcgt
      481 ccacaatgat ggctgctcta gtgggtttgc tgaaagagca tttggctctg ccgctctgcc
      541 gctcacaaaa tacgagattg ctgatctcgc tcattctatc gagcgacatg acctcgcaat
      601 ccaaggaggg ctgcaggatc attttgcggc cactttcgaa ggcttcaatt tcatcgaatt
      661 tggcgagcgg accctagtaa atccgcttcg gataccggat gatatcgctg cagagttgca
      721 actgaacctc ctcttggtgt atactggcaa agtcgcggcg ggcggtaagg taacaggtgc
      781 aggcggcggg gggcatgtac tattttactg tgacttcgaa cgcaagcata aagttgctga
      841 agccttgatt gccatggggg ctaccgtggc ggacttcaac ttcgacagta agggtcttgt
      901 cacgtggcga acgcgctaga gactgccgag gaagttctgg aatcgcgact tcgcgattat
      961 cggggcagtc tggacctgct agctggtcat gaggctttga aggcagccgc tgaggccgcg
     1021 gaactgatat gggacgtcat atgcagtgga ggaaagacca tcttcttcgg caatgggggg
     1081 tcgtcaatgg acgctggcca tcttgccgct gagcttctcg ggcgcttcta tttcgagcgg
     1141 ccagctattg cggcgctctc actcgcggat gtcacggccg ccgtgacggc tatcggcaac
     1201 gattacgggt atgagtacgc cttctcccga cagcttgagg gtgtcgggca cgttaatgac
     1261 gtcgcgatcg gcttatcaac aagcggtaac tcacccaatg tggtcaacgc ctttgcagtc
     1321 gcccgcgaga aggggttaag gactgtcgcc ctaacgggga gcgggggcgg ccgggtagcc
     1381 gccatggcgg acgttctcgt cgcagtcccg tcgagtgaca ccccgcgggt acaggagatg
     1441 tgcatgcata taggtcacag catctgcgag tacgtggaag cgcgatggct ggcgagtgcc
     1501 ctgtagcagg tgtatgtgtc gccttcttgg atcgtgacgg caccatcaac gtcaaaaagc
     1561 cggagggcca gtatgttctg gcgcctcagg aggttaactt ggtgccgggt gctggacgcg
     1621 cggttcgaga actgaacgac gcaggctggc gcgtgattgt cgtcacgaac cagcgcgcac
     1681 tcgcgcgcga gttgatgtcg aatggggacc ttgcgcgagt acatgcccga cttgatcagc
     1741 tactcgcggg gttcggggcc cgggtagacg agtaccgcgt ctgcccgcat cgggagggca
     1801 catgttggtg ccgtaagccc catccgggcc tgcttaaaga gtatatgtcg gcgcacgctg
     1861 acgtagatgt gcgaaagtcg tggatggttg gcgactcgga gtccgatgtc gaggcaggta
     1921 tgcgtgcttc agttcggacg ctccgtctgg ccggaggcgc aacggccact cgggcggagc
     1981 gtgttgaacc ggacttgtcc cacgcggtga agtttatgct cggcgtaagc ggctcgtagt
     2041 gcgatatgag gcccctgcgg tggttggacg gttgctggca cagccctgaa acctggatgc
     2101 accgcgcaac gttcgatctt gtctggctcg gcgttctgat cttgaccccg ggtgtgggcg
     2161 tgccggctgg gccggtctgc ccggcttttc cacttcggtt ccggttgggc ctgtcggcgg
     2221 gt
//
LOCUS       DASRKK010000524         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1217092, whole genome shotgun sequence.
ACCESSION   DASRKK010000524 DASRKK010000000
VERSION     DASRKK010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217092"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFC15_12030"
     CDS             complement(<1..1005)
                     /locus_tag="VFC15_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZM10927.1"
                     /translation="MDDKAEKVALFRYGLIAPLVLETLPRGELTRRAQQIAARLYDIP
                     HSARRQVSVDTLLDWTLRYRRNGLAALSPQPRQDRGQARAVAPEVATLIERLKRENPH
                     RTGAALLRELALANEPKEPSLSASTLYRFLRARGLTERQLLLDKATAHKKYEAQFANQ
                     IWQSDMLFGPWVGRPGGGKQQVFLQAALDDASRLIPHAQFYPNQGLDAFLDCLRQAIA
                     ARGIPTRLYMDNAKIYRSPQLARIAASIGILIVHTPPYQPEGRGKIERFFRSVREQFL
                     ASLDPKTLLSLEQLNERLWHWLDTVYHRREHSSLQTTPLVRWQRDIEQVRQLPPATDL
                     R"
     gene            complement(1083..1685)
                     /locus_tag="VFC15_12035"
     CDS             complement(1083..1685)
                     /locus_tag="VFC15_12035"
                     /inference="COORDINATES: protein motif:HMM:NF040103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HZM10928.1"
                     /translation="MQILHPFVGTVQQYTEQLADPDCYRPGHCPQCHTKHPLTAHGFY
                     TRTLIDTAFDGIIRVRRYLCQACQRTVSLLPEFVLPYLRSSLTVIALFLVTRLLLAQT
                     LNRAAATAPQPMPYQRGQFWIRRFRAQAEALCAALAALTQPTPAPDFVHRALAMLEST
                     GWIGAHRFLFAGVRCHLLGWSRSLAPDGRRAAFSSAAAPA"
     gene            complement(1951..2481)
                     /locus_tag="VFC15_12040"
     CDS             complement(1951..2481)
                     /locus_tag="VFC15_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM10929.1"
                     /translation="MQNYPDCGTIGYQTFSANPVLDGDTVVGIEGFVVDITERKRMED
                     ALRRSEERFAKAFLSSPAIIAIVDLAEDGRFVDVNEAFELATGYGREEVFGHTTTELG
                     LWADLGNFDEGEKQFRASGRLRNFEHRFRRKNGEIGTGLTSAEVIELDGKPFVISATI
                     DITEQEKTEGALRSLV"
     gene            complement(2378..>3975)
                     /locus_tag="VFC15_12045"
     CDS             complement(2378..>3975)
                     /locus_tag="VFC15_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM10930.1"
                     /translation="PILDHGKTTGISVFGKNITERKRAEQELQQAEREYRTIFENAVE
                     GIYRTSPEGKGLAANPALALMLGYESAAEGMSMVSDVGSQVWLDPSERAKFLHLLDTH
                     EVVRNYECQLKRKDGTAVWVSINGRRVCGPDGQMVYTEGFVEDITERRAAEKRLQAQG
                     KRFQDIIEHTDAGYFRIGTGGCFENVNPAWLRMHGFTRKEDIIGLHFSAVHIPEDVAE
                     AQEIVETLMRGEARRGELSTLRQDGTIGYQTFSANPVLDGDRVVGLEGFLVDITAWKT
                     AEEEKRRTLQRANEVVAKAEAHYRLMFNSVSDAVFVHKFGEDGRPSCFLEVNDNACRL
                     LGYTREKLLQMRVADIIAPEEHFNVSANAKRFLADGHATWEGRFAAQDCRRIPVEVNA
                     RVFNLDGSPTIMSSVRDISERKDAEARLQAQNERFRRIIENTDAGYFRMGLDGRWEDV
                     NPAWLRMHGFANREEAIGLHFSAVLNPEDQAKAKEIGQTLMRGESVRGAELPRLRDHW
                     LSDVLSQSRTRRRYGRRHRRLRR"
BASE COUNT          758 a         1138 c         1234 g          845 t
ORIGIN      
        1 ccgtaaatcg gtagccggcg gaagttggcg gacctgttcg atatcgcgct gccagcgcac
       61 cagtggtgtg gtctgcaaag agctatgttc gcggcgatga tagacggtgt cgagccagtg
      121 ccacaagcgc tcgttgagtt gttccaggga gagcagcgtt ttgggatcga gtgaggccag
      181 aaactgttcg cgcacggaac ggaagaagcg ttcgatcttg ccgcggcctt cgggctgata
      241 cggaggggtg tgcacgatga ggatcccgat ggaagcggcg atgcgggcca actgcggcga
      301 gcggtagatc ttggcgttgt ccatgtagag gcgagtgggg atgccgcggg ctgcgatggc
      361 ctggcgcagg cagtccagga aggcgtcgag tccctggttg ggatagaact gggcgtgcgg
      421 aatgaggcgg ctggcgtcgt ccagcgcggc ctggaggaag acctgctgct tgcctccgcc
      481 cggccgcccg acccacgggc cgaacaacat gtcggactgc cagatctgat tggcaaactg
      541 cgcttcgtac tttttatgcg cggtagcttt gtcgagcaac agttgacgtt cggtgaggcc
      601 gcgggcgcgc aggaaccgat agagcgtgga agccgacagg ctgggttcct tcggctcgtt
      661 ggccagggcc aactcgcgca gcagagcggc cccggtacga tgcgggttct cgcgcttgag
      721 ccgttcgatg agggtagcga cttccggcgc gacggcacgg gcttgcccgc gatcctggcg
      781 tggttgggga gacagcgcgg ccaggccgtt gcggcgatag cgcagcgtcc agtccagtag
      841 cgtgtccacc gagacctgac ggcgggcgga atggggaatg tcgtagaggc gggcggcgat
      901 ctgctgcgcg cgtctggtga gttcgccgcg gggtagcgtt tccagcacca gcggcgcgat
      961 caggccataa cggaagagcg cgactttttc ggctttgtcg tccatggagt tgtttttttc
     1021 tcctggacgg cagcttacgg cggaaggctt cgcggttcca tgcaaatggt gtgtggggaa
     1081 gctcaggcgg gcgccgcggc ggaggagaat gcggcgcggc ggccgtcggg agccagactg
     1141 cgggaccagc ccagcaggtg gcagcgcacg ccggcaaaca gaaagcggtg agcgccgatc
     1201 cagccggtgg attcgagcat ggccagggcg cggtggacga agtcgggcgc gggagtcggc
     1261 tgggtcaacg cggccagagc ggcgcacaac gcctcggctt gcgcgcgaaa gcggcgaatc
     1321 cagaactggc cgcgctggta gggcatcggt tgcggcgcgg ttgcggcggc gcggttcagt
     1381 gtttgcgcca ggaggagtcg cgtgacgaga aacagcgcga tcaccgtcag gctgctgcgc
     1441 aggtacggca aaacgaactc gggcagcagg gagacggtgc gctggcaggc ttggcataag
     1501 taacggcgca cgcggatgat gccatcgaaa gcagtgtcga tgagcgtgcg cgtgtagaag
     1561 ccatgcgcgg tgagtggatg tttggtatgg cactgcggac agtggccggg gcggtaacaa
     1621 tcggggtcag ccagttgctc ggtatattgc tgcaccgtgc caacgaacgg gtgtaggatc
     1681 tgcataggat ctgcctctgg gagcctcgaa ctcccaaggt tcgttctgcg ggcgggagag
     1741 ctcttttgga tgggtgtccg tgggagttct tccgctcacc tcgatcctac acatctgaga
     1801 aagtcacgaa aagggaaggg atagcgcgtc ggctgccgat cctgcggagt ttggcacgct
     1861 cgggcccggc gccgcttctc gatcccctcc ggcgcggcgg gaactaatct gagaaaaact
     1921 gctcgctaca aggggaagaa ttgcgacccg ttacaccaga cttcttaggg caccttcagt
     1981 cttctcctgt tcggtgatgt caatggttgc cgaaatcaca aacggtttgc cgtcgagttc
     2041 aatcacttcc gctgaagtca agcctgttcc gatttcgcca ttcttcctgc ggaaacggtg
     2101 ctcgaaattg cgaagacggc cagaagcacg gaactgtttt tcgccttcat caaagttgcc
     2161 cagatcggcc cacagcccaa gctcggtcgt cgtatgtccg aatacttcct cacgcccata
     2221 gccggtcgcc aactcaaacg cctcattgac atcgacgaac cgaccatctt cggcgagatc
     2281 gacaatggca atgattgcag gactggatag aaacgccttg gcaaaccttt cctctgacct
     2341 ccgtaaagcg tcctccatcc gcttgcgctc ggtgatgtca acgacgaagc cttcgatgcc
     2401 gacgaccgta tcgccgtcga gtacgggatt ggctgagaac gtctgatagc caatggtccc
     2461 gcaatctggg tagttctgca cctcttaccg attcgcctcg catgagagtt tggccgattt
     2521 ctttcgcctt ggcctgatcc tctggattca gaactgcgga gaagtgcagc ccaatggctt
     2581 cctcccggtt ggcgaatccg tgcatccgca gccacgcagg gttcacgtct tcccagcggc
     2641 cgtccagtcc cattcggaaa tacccggcgt cggtgttctc gattattctg cgaaaccttt
     2701 cgttttgggc ctgtagccgt gcttccgcat ccttacgttc cgaaatgtcc cgtacagaag
     2761 acatgatcgt tggcgaacca tccaaattga aaacacgagc gttgacttct accggaatgc
     2821 gccgacagtc ctgcgctgca aaccgcccct cccaggtcgc atggccgtca gccagaaatc
     2881 tcttcgcatt ggctgacaca ttgaagtgct cctccggggc gataatatcg gccactcgca
     2941 tttgtagcag cttttctcgt gtgtagccca gaagacgaca ggcattgtcg tttacctcaa
     3001 ggaagcaact tggtcgcccg tcctctccga acttgtgaac gaagactgca tccgaaacac
     3061 tattgaacat gagacggtaa tgggcttccg ctttggcgac aacttcgttt gctcgctgaa
     3121 gtgtgcggcg cttctcttct tcggctgtct tccaggcggt gatgtcaacc aggaaacctt
     3181 cgaggccgac tacccgatca ccgtccagta cgggattggc tgagaaggtc tggtagccaa
     3241 ttgttccatc ctgacgcaat gtggacaact cgccccttct tgcctcaccc cgcatcaggg
     3301 tctcgacaat ctcctgcgct tcggctacgt cctcgggtat gtgaactgcc gagaagtgca
     3361 agcctatgat gtcttccttg cgggtgaatc catgcatccg cagccacgca gggtttacgt
     3421 tctcgaagca gccgcccgtt ccgatgcgga agtatccggc gtcggtgtgt tcgatgatgt
     3481 cttgaaacct tttgccttga gcctgtagcc gcttctcagc tgcccgccgt tcggtaatat
     3541 cttcaacaaa tccctcagta tagaccatct gcccgtcagg accgcacact ctccggccat
     3601 tgatcgacac ccaaacggct gtaccgtcct tgcgcttaag ctggcactcg tagttacgga
     3661 cgacttcatg tgtgtcgagc aggtgaagga acttcgcacg ttcagatggg tccagccaca
     3721 cctggctccc cacatccgag accatcgaca tgccctccgc cgcagactcg tagcccagca
     3781 tcagagccaa ggcggggttt gctgctagac ccttcccttc aggcgaggtt cggtagattc
     3841 cttcgaccgc attctcgaag atggttcgat attccctctc agcttgctgt agctcttgct
     3901 ctgcacgctt acgttccgtt atgtttttcc cgaaaaccga aatgccggtc gtcttgccgt
     3961 ggtcgagtat tggac
//
LOCUS       DASRKK010000525         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1413396, whole genome shotgun sequence.
ACCESSION   DASRKK010000525 DASRKK010000000
VERSION     DASRKK010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413396"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..2030
                     /locus_tag="VFC15_12050"
     CDS             429..2030
                     /locus_tag="VFC15_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM10931.1"
                     /translation="MKHRHQLVVVFGLALLCVSIGNSLWAQDSVVVTVSATGAGNAPL
                     MTLGRPDFTVQESGKPRAITTFVGPDAKPAAPPKLQLNEFSNLPDFREVSGAIFVVLD
                     TVDTRYLDERDSRPLILKFLGKAAQAKHAVTLGILSDKGLHLYHDYRTGSDVLLAALI
                     KAGLGGMKGVAPPPGVNDAEVDAEAARLTAFSKGDLSNSTAALQLLRSSIDMPLTMFQ
                     DVAFASEGLPGRKSLVWMTNAVPFDIDPKTMQFKSPQIAGQGVAVSGLTVGGAKNSLT
                     DSEIKRITPIWRRAIRALFDGGVAVYPVEVNGATTAASSSFTQQRMKELALLTGGKAF
                     YGSNDPFPDILLSSTGNTAGYELGYSPDAGASPDFRRLEVTVNQQNVEVNHPSGYFPS
                     EGAPKTRAGDEIGIAMTSPLEYTGIRFKAAVTSIDDGSAGKKKVNLLISLPGNAGILN
                     ETTGAVDVGFVAVATNAKGDKVGTMNEGGGGKFPPEAIAQIKEMGFQLKRSFEASPGE
                     CTVRFLVRDNQTGRMGDVFFPLNVK"
     gene            complement(2043..4208)
                     /locus_tag="VFC15_12055"
     CDS             complement(2043..4208)
                     /locus_tag="VFC15_12055"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZM10932.1"
                     /translation="MIELPNPMQIRLALRRTLRPTSKLGKTTLWFGGLAVGLQLWKWI
                     AHSRSGSMLSVWAALVTYVFVVCLMVRGLRWARQKLMWRLRNRLIVTYVFIGVIPVVL
                     LISMVLLAGYLFAGQFATYIALSDLDSELQHLTAVNNALAEQFRTLTQSGKLTPQLAA
                     EIANSSDESLRQRTLTVLEGDNGFVIEPGGKITIVQLKLPQTARGSINRFVMDHGRLH
                     LRTVRRVEAGSRHMIILSSLPITSELLQRAAHVGSVDLYLPDQDMDVDKPASLSSGNA
                     TPTTESTESFAAPKSLAGRSATSAPQNIAAGRVAPAATRFDPEFRWWTPFNIVDWESG
                     KELTGVIVVGTRPSMLYNVLFGNLNTKAGIFIQVLLGIALFFGLIELIALFIGVRLTR
                     SVTKSVAELYTATQHVNRGDLSHRIEVKSRDQMAALEGSFNSMTASLARLIAEQKEKQ
                     RLEGELAIAHEVQELLFPRQISELSTLEVHGVCRPARTVSGDYYDFIPLHADRMVLAV
                     GDISGKGISAALLMATVHAFVRAYSLEPELALAAVATAGGARTGDLQLYYRGNGDRDN
                     RLSPGMLMATLNYQLYRSTPPEKYATMFLANYDAGARMLTYSNAGHLPPYVVGGDGSV
                     TQLDTSGTVVGLFDSMSYEESRIPMRPGDVFVAFSDGVTEPENEFGEFGESRLVDLIR
                     LHRDQPLSRISDVVTGAVADWIGGNEQPDDVTLVLARAR"
     gene            complement(4372..>4622)
                     /locus_tag="VFC15_12060"
     CDS             complement(4372..>4622)
                     /locus_tag="VFC15_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10933.1"
                     /translation="RAIPRYGVGRGGKIDFMAKLTLVYGMRLLPSEEIEEVGEAQVKL
                     KNGREARVSLHIVEGSKDDIEKQLRTSIDAFFDLYPEI"
BASE COUNT          977 a         1356 c         1327 g          962 t
ORIGIN      
        1 cctgctaagc gaggtaggag caaaatggcg gaagccgaag aacaatcacg cgaagcgcgg
       61 cggcgcaagg ctgtggaaaa gccgctccgt ggaaaagtcc acaaaacgga ctttcccact
      121 acgcttggaa atccgcacac aacgcggatt tccactttac gcacagcctc aactactact
      181 ggacaataaa aatgcaccac cacgacaaaa ggggacattt ctaatgtctt aaacaagggg
      241 acatttctaa tgtccgttga cacatgtgag cattgtcatt gccgaggttc acgcgatgcc
      301 gtaaactttc cgcgcatcgg attaatgggt tactcataaa ttaacggctc gcaaacgcat
      361 ggcccacagg gcctgtcggg acgacatcgg cgagtcccgg ctggttgcga gcagcgaggg
      421 gattgttcat gaagcatcgt catcagttgg tggttgtttt cggtctggcg ttactctgcg
      481 tgtccatcgg aaattctctg tgggcgcagg acagtgtcgt agttacggtc agcgccaccg
      541 gcgcgggcaa tgcccctctt atgacgctgg gaaggccgga ttttaccgtg caggaatcgg
      601 gcaagccgcg cgctatcacg acatttgttg ggccggacgc caagcccgcc gccccgccaa
      661 agcttcagct caacgaattc agcaatctgc cagattttcg cgaggtcagc ggtgcaattt
      721 tcgttgttct ggacacggtt gatacccgct acctggatga gcgcgattcg cggccactga
      781 ttttgaaatt tctgggcaaa gcggcgcagg ccaaacacgc agttacgctc ggcatcctga
      841 gtgataaggg gctgcatctc tatcacgact atcgcaccgg ctcagacgta ctgttggccg
      901 ctctcatcaa ggccggcctg ggaggcatga aaggtgttgc tcctccgcct ggcgtcaacg
      961 atgcggaggt ggacgccgaa gcggcgcggc ttacggcgtt cagtaagggc gatctgtcca
     1021 actcgaccgc ggccctgcaa ctgctgcgct ccagcatcga catgcccctg accatgtttc
     1081 aggacgtcgc tttcgcctcc gagggccttc ccggacgtaa gtctctggtt tggatgacca
     1141 acgcggtccc tttcgacatc gacccgaaga ccatgcagtt caagtctccc caaattgcgg
     1201 ggcaaggcgt tgcggtttca ggtcttactg ttggaggcgc caagaactcg ctgactgatt
     1261 cggagattaa gcgcatcacg ccaatatggc gtcgcgccat tcgtgcgttg tttgacggtg
     1321 gagtggccgt ctatcccgtc gaagttaacg gtgccaccac cgcagcttcg agttcattca
     1381 cccaacagag aatgaaagaa ctggccctgc ttaccggagg caaggccttt tacggcagca
     1441 acgacccgtt ccccgatatt ctgctgagca gcaccgggaa taccgctggc tatgaactcg
     1501 ggtattctcc ggacgcaggt gccagccccg atttccgtcg cctggaagtc acggtcaatc
     1561 agcagaatgt tgaagtcaac catccttcag ggtatttccc ctctgaggga gctccgaaaa
     1621 cgcgcgccgg cgacgagatt ggcatagcca tgacctcacc cctggaatac acagggatac
     1681 gcttcaaggc ggcggtcacc agcatcgacg atggatcggc gggcaagaag aaagtgaatc
     1741 tgctcatcag ccttcccgga aatgctggca tcttgaatga aacaacgggc gcggtggacg
     1801 tcggatttgt cgctgtcgct accaacgcta aaggggacaa ggtcggaacc atgaacgaag
     1861 gcggcggcgg aaaatttccg ccggaagcga tagcacagat caaggaaatg ggcttccaac
     1921 tcaagcgatc atttgaagcc tctcccggag agtgtactgt ccgcttcctg gttcgcgata
     1981 accagacagg gcgcatggga gacgtcttct tcccgctgaa tgtgaagtaa gcgcttttgc
     2041 ggtcagcgtg cgcgggccag cacgagcgtc acgtcgtcgg gttgctcgtt gccgccaatc
     2101 cagtctgcga ccgctcccgt cactacgtcg ctgatgcgcg acagcggctg atcgcgatgg
     2161 aggcgaatca agtcgaccag gcgggattcg ccaaattccc cgaactcatt ttctggctcc
     2221 gtcaccccgt cgctgaacgc gacaaagacg tccccgggcc gcatgggtat gcgcgattcc
     2281 tcgtagctca tgctgtcgaa cagaccgacc accgttcccg aggtgtccag ttgggtcacg
     2341 ctgccgtctc cgccgacgac ataaggaggt aaatgtcccg cgttggagta ggtcaacatg
     2401 cgcgctccgg cgtcgtagtt agccagaaac atggttgcgt acttttccgg aggggtgctg
     2461 cgatagagct ggtaattcag tgtggccatc aacattccgg gagaaagacg gttgtcgcgg
     2521 tcgccgttgc cgcgatagta caactgcaga tctccggtgc gcgcgccccc cgcggtcgcc
     2581 acagcagcca gcgctagctc gggttccagc gagtacgcac gcacgaaagc atgcaccgtg
     2641 gccatcaaca gcgcggccga gattcccttg ccgctgatgt cacccacggc cagtaccatg
     2701 cggtcggcat gtagggggat gaaatcatag taatcgccgc tcacggtgcg ggcggggcga
     2761 caaaccccgt gcacttccaa tgtgctcagc tccgaaattt gccgcggaaa gagcaattcc
     2821 tgcacttcgt gcgcaatcgc cagttcgcct tccagccgct gtttctcttt ctgttccgcg
     2881 atcaatctcg ccagagaggc ggtcatggaa ttgaacgacc cctccagggc agccatttga
     2941 tcgcggctct taacttcgat tcggtgactg aggtcgccgc gattcacgtg ctgggtcgcg
     3001 gtataaagtt cagccaccga cttcgtcacg ctgcgagtga gccgcacgcc gatgaacaac
     3061 gcaatcaatt cgatcagccc gaaaaagagg gcgatgccca gcagcacttg gatgaagatt
     3121 cccgccttgg tattcaggtt gccaaacaac acgttgtaca gcatcgacgg ccgcgtgccg
     3181 acaacaatga ctcccgttaa ctccttgccg ctctcccagt ccactatgtt gaatggcgtc
     3241 caccatcgga actcgggatc gaagcgcgtg gccgcaggag ctacccgccc cgccgcgata
     3301 ttttgcggtg cgcttgtagc gctccttcct gccagtgact tcggagccgc aaagctttcc
     3361 gtggactccg tagtgggggt cgcatttccg gacgacaacg aggcgggctt gtctacgtcc
     3421 atgtcctggt caggcagata gagatcgacc gaaccgacgt gcgccgcgcg ctgcagcaat
     3481 tcggaagtga tcggcaggct cgacaggatg atcatgtgac gcgagccagc ctcgacccgt
     3541 ctcacggtcc gcaagtgaag ccggccgtgg tccatgacga agcggttgat gctgccccgt
     3601 gctgtttgtg gaagcttgag ctggactatg gtgatcttgc ctccaggttc gatcacaaat
     3661 ccgttgtcgc cctcgaggac agtcaacgta cgctgtcgca aactctcgtc cgacgagttc
     3721 gcgatttcgg cggccagttg cggagtcaac tttcccgact gggtcagcgt gcggaattgt
     3781 tccgccagcg cattgttgac ggcggtcaaa tgctgcagtt cgctgtcaag gtccgacagc
     3841 gcgatatagg tggcgaattg tccggcaaag agataacccg ccagcagcac catcgagatc
     3901 agcagcacga cgggtatgac gccgatgaat acataggtga caatcagacg gttgcgtagc
     3961 cgccacatca atttctggcg tgcccaccgc aggccccgca ccataaggca gacgacaaat
     4021 acataggtga cgagggcggc ccacacgctc agcatggaac cgctgcgtga atgcgcgatc
     4081 cacttccaca gttgcagccc taccgccagg ccgccaaacc agagagttgt ctttcccaac
     4141 ttgctggtgg ggcgcaatgt ccgccttagc gcgagtcgta tttgcatcgg gttgggaagc
     4201 tcaatcacgg actgcctgcg ttctttctgg gtggtttagg ggattatatc tgtgacgccg
     4261 cgggcgccac ctgcactgta ttacggtttc ccgaatggta acgttcccgg cagccgacgg
     4321 cctgcgctgc ggcggggtac aactcgctcg cgtgagcgta ctcgactgcg gttagatttc
     4381 cggatagaga tcgaagaagg cgtcgatgct tgttcgcagt tgcttttcga tgtcatcctt
     4441 cgatccctcg acaatatgaa gactgacgcg cgcctcgcgg ccattcttaa gcttgacctg
     4501 agcttcgccc acctcttcga tttcttctga aggtagcaga cgcatcccgt acaccaatgt
     4561 gagctttgcc atgaagtcga ttttaccgcc ccttccgacc ccgtatcggg gaatcgcgcg
     4621 aa
//
LOCUS       DASRKK010000526         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_923841, whole genome shotgun sequence.
ACCESSION   DASRKK010000526 DASRKK010000000
VERSION     DASRKK010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923841"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFC15_12065"
     CDS             complement(<1..412)
                     /locus_tag="VFC15_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HZM10934.1"
                     /translation="MSRPPLEMADIVRCAGQAFLEHSRKWINWQHQKVLLAIARCRTA
                     ALGGHRDQCSDCGHTAISYNSCRNRHCPRCQGNARLRWLKTREQELLPTRYVHAVFTL
                     PRELAPLALQNKRLIYNLLFHASAETLLAIARDPR"
     gene            complement(409..1293)
                     /locus_tag="VFC15_12070"
     CDS             complement(409..1293)
                     /locus_tag="VFC15_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM10935.1"
                     /translation="MTHLRQIMLEELRRRNFADTTIRSYLHGVEHFSRYFRRRPDQLG
                     PEDIRKYQAMLFTQLKFSPNTVILRLASLRFFYIHVLKRGWSIAETPYPKKVRHLPQV
                     LSQEEVARLIEAAESPFHRILLMTLYATGARRAEAAHLKIGDIDSQRMVVHIRGGKGG
                     LDRDVMLSPKLLEALRTYWRGLRRKPTEWLFPGNRWHTASYPVTTKVLWTACQNAAER
                     AGLAHKHIHPHTLRHCFATHLLEAGADLRTIQILLGHRDLEVTTIYLHLSQRHLSATA
                     SPLDALTFSAQGGHKHSS"
BASE COUNT          639 a          908 c          869 g          556 t
ORIGIN      
        1 gccggggatc gcgagcgatc gcaagcaagg tctcggcgct ggcatgaaag agcaggttgt
       61 aaatcagccg cttgttctgc aaggcgagcg gggctagttc ccgcggcaag gtgaagacgg
      121 catgtacata gcgagtgggc aggagttcct gttcccgcgt cttgagccag cgcaggcgcg
      181 cgttgccctg gcagcgtggg caatggcgat ttctgcacga gttatacgag atggcagtat
      241 gtccgcagtc tgagcactga tcgcgatgtc cgcccagcgc ggcggtgcgg cagcgtgcaa
      301 tggctagcag cactttttgg tgttgccagt tgatccactt gcgactgtgt tcaagaaatg
      361 cctgtccagc acagcgaacg atgtcggcca tctccagcgg aggccggctc atgaactgtg
      421 cttgtgtcct ccttgcgcag agaacgtgag tgcatcgagc gggctggcag ttgcgctgag
      481 atgccgttgg gagagatgca gatagatggt agttacttcg aggtcgcgat gaccgagcag
      541 tatctggatg gtgcgcaggt cggctccggc ttccagcagg tgggtggcaa agcagtggcg
      601 tagcgtgtgc ggatggatgt gcttgtgcgc caagccggcg cgctcagcgg cattctgaca
      661 ggcagtccag aggaccttgg tggtgacggg ataacttgcg gtatgccatc ggttgccggg
      721 aaacagccac tcggtcggct tgcgtctgag cccgcgccag taagtgcgca gcgcttcgag
      781 aagcttcggg ctcagcatga cgtctcggtc caggcctccc ttgcctcccc ggatatggac
      841 caccatgcgc tgactgtcaa tatcgccgat cttcagatgc gctgcttcgg cgcggcgtgc
      901 cccggtggca taaagcgtca tgagcaggat gcggtggaag gggctttctg cggcctcaat
      961 cagacgcgca acctcctcct ggctgagtac ctgcggaagg tgacgaacct tcttcggata
     1021 gggcgtctcg gcgatgctcc agccgcgctt caacacgtga atatagaaga agcgcaacga
     1081 agccaaccgc aggatcacag tgttgggact gaacttcaac tgggtgaaga gcatcgcctg
     1141 atacttgcga atgtcctcag ggccaagctg atcgggccgg cggcggaagt accggctgaa
     1201 gtgctccacc ccgtggaggt acgagcggat ggttgtatcg gcgaagttgc ggcgtcgcaa
     1261 ctcctcaagc atgatctgac gtagatgggt cacagagagt tcctcctctg cgacccaaac
     1321 tagctcacat caccgcgaca ctgccggctc aacaacacgc gaatgcgtcc gccgcaaagc
     1381 ggcttcgttc aagtcggcca gcatcccgca accgcattgg caccgccact tggatgtaaa
     1441 acgccgaaac cgcccaactg gctttctcgc cgctgatttt cgcggattgg taacggtaaa
     1501 cgccctactc gtagaagtgc aacccctgac tccccggaag tctggttgtg ggagatctcc
     1561 cgctgacaat tcaccgagat ttcccccgac cgacggacaa acgggaacgt gtggtcgccg
     1621 ctcgatcgct agcggtgcat gagtcccaag agcaagtcca tcacccgagc gtcgccacaa
     1681 ttccatgact tccactgcaa ccaggacaag ccgtagtttg aacgttcgca catcagatgt
     1741 gcattctcgt tgcaaccgga atcggcagcc gagccatggc cagcaggtgt gcacaacatt
     1801 gctgccacgt cgtcgcacag caatccgccg tcaggacaac ggccgtcacc caactctgcg
     1861 tccaaacaaa ccgctgcagt acgcacaacc cgcgcacctg ccggtgcacc gacagccgcc
     1921 cggatctggt gtgcatcctt gcacagcacc ttgcacacgg cggtgcagac cgaccacgca
     1981 gtgtgaatct ctaaccggtt taaacttgcc atccgtcgtg caccggcccg tgcatacggt
     2041 tcggcaacga cccgtgcatt cgcaacgaca attggcaacg gctgtgcagc ccattgccgc
     2101 ccgtaccagg gccgcagcct gcgcaactgt cgcgcaagtc tgccacatat tctcgtcgcc
     2161 gccggcatcg gcagcgcctg catctggagc gcatccttgc acagcacctt gcacacggcg
     2221 gtgcgcgtcc accccgcagc ttggatctcg aaccggtttc aacttgccat ccatcttgca
     2281 ccgctccatg cacacgcttc cgcagcaacc cgcacgctta atgaccagcc atggccagca
     2341 ggtgtgcaaa tcattgctgg caagtcgtgg cacaggaatg cgccgtcggg acaacagtcc
     2401 ggcacgcgct gtgcgtccat ccacaccacg gtcgtacgca caacccgcgc gccactcggc
     2461 gcaccggctt gcgtgactct tgggcgggtg cagtcgcgca acggtcggaa catcgaaccg
     2521 gatcaagcgc acatcagcaa caccaactgc atcgccgcag cagcacaccg cgcatcacga
     2581 gtgtcgcagc cactccgtcg ttttccacgg aaaacgctac cgaacagggg atagtcaact
     2641 gctaaactat agaatccagg aagtttacgt gaggtccctc aaccgcaata caacgccaaa
     2701 ccaaccacaa tccaactgca ataagtgaaa aggcaggatc ttaggcaaca ggaagagtct
     2761 tcccatattg acccgtgggc ttcactgtca gcacggagca tagcgtggtt gatactggcg
     2821 accggttgct tcttactgtc aggttaagcg tgtgggtaag gaagtcctcc cacaggccaa
     2881 agtgaaccgg gcgtcggcag ctttcgaacc tccgaaccag gaacaacagc aggtcgtggt
     2941 tcggagcaag gtacagaaaa tgggcgatgc cc
//
LOCUS       DASRKK010000527         6772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1022174, whole genome shotgun sequence.
ACCESSION   DASRKK010000527 DASRKK010000000
VERSION     DASRKK010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022174"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2876)
                     /locus_tag="VFC15_12075"
     CDS             complement(<1..2876)
                     /locus_tag="VFC15_12075"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10936.1"
                     /translation="MAQQKTANRNRFDFSKIPATIQIPNLIEVQKRSYDRFLQMDSLP
                     SERDDSGLQAVFQSVFPISDFRNVSQLDFVEFAIGNWECKCGHLKGLNHLRTTCRNCG
                     STVKTDPFHLGDVLCTKCGTYNANTPDFCNKCGDPVGLQLKYDVAECEERGMTYSAPL
                     KVTMRLTIFDKDPETGNRSVRDMKEQEVFFGDIPLMTQNGTFIINGTERVIVSQLHRS
                     PGVFFENANNRTYFLGKIIPYRGSWVEFEYDQKNILYVRIDRKRKFLGSIFLRALGLR
                     SDEDILRTFYTIDRIALRDNKIYWTLEPGVDRPTNLLGMKLAHKIATKSGEEIAHSGR
                     KVTASVMKEILKHKVSEIEIDTTDLEGAFVAADVIDTATGEVMLEANTELTTDKLSKM
                     LEASVAELHLFFPERDDVGTVISQTLRRDSVKTPQEALIEIYRKLRPGDPPTLDTATS
                     LFHGMFFDSRKYDFSRVGRLKFNIKLFESEDATHLDHRTLEPDDFYGTIRYLLKLRKN
                     IGSVDDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNA
                     KPVMAAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPT
                     HYGRICPIETPEGPNIGLISSLSCYARINDYGFIESPYRKVKAGHVLDFVSVVNAGDS
                     DRRVGDHLEKAEVIKLNNELRDRKKKLIDYEPFSFYLSAWEEDRHVIAQANAEIDDKG
                     RIVAELVNARKAGNFVLVNRNEVDYIDVSPKQLVSVAASLVPFLEHDDANRALMGANM
                     QRQSVPLLRAQAPIVGTGMEGVTARDSGAVVLARRSGIIDSVDSERIIVRVEGEHHPM
                     QLSREVGSDIYQLTKFKRSNQNTCINQRPIVKKGTRVAKGQVIADGPCTDMGELALGR
                     NVLVAFMAWRGYNFEDAILVSEKMVKDDYYTSVHIEEFEIESRDT"
     gene            complement(3478..3861)
                     /gene="rplL"
                     /locus_tag="VFC15_12080"
     CDS             complement(3478..3861)
                     /gene="rplL"
                     /locus_tag="VFC15_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZM10937.1"
                     /translation="MADLQQLEETIVGLSLLDAAALVKSLETRLGVSAAAAAPVMVAG
                     AGAGAAAAAPVEEKTEFAVILTAAGANKINVIKAVREVTSLGLKEAKDLVDGAPKTVK
                     EGITKDEAAAIQKKFVDAGASVEVK"
     gene            complement(4056..4604)
                     /gene="rplJ"
                     /locus_tag="VFC15_12085"
     CDS             complement(4056..4604)
                     /gene="rplJ"
                     /locus_tag="VFC15_12085"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZM10938.1"
                     /translation="MAVTKAKKIEQVEKLSHDLQGASHAIVGTFTKLTVEKDYELRKA
                     VRSAGASYRVVKNTLAERAAKGTKVEDAVKGLAGVTSIAYTSGDPVALAKVLSKYAKD
                     HPEYTFKAGIVDGRVIAIKDIDALASMPSKEEIYSKLLFLMSAPAQRLVTVINAIGRD
                     LAVVINQGVEKNKFAGEEAASA"
     gene            complement(4688..5395)
                     /gene="rplA"
                     /locus_tag="VFC15_12090"
     CDS             complement(4688..5395)
                     /gene="rplA"
                     /locus_tag="VFC15_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZM10939.1"
                     /translation="MKKSGKNVEKARAGVENHPYTLQEAVPLLQKVKFAKFDETVDLT
                     MRLGVDPKHADQMVRGTVILPHGLGKTKKVLVIASGEKQREAEAAGADFVGGEDMVEK
                     IQKENWTDFDAVISTPDMMKSVGRLGKVLGPRGLMPNPKTGTVTFDVAAAVKETKAGK
                     VEFRTDKTALVHVPVGKISFDPQKLVENAATVITSVIKAKPSVAKGKYIKACYLSSTM
                     GPGVAIDIAAVELAAKS"
     gene            complement(5503..5934)
                     /gene="rplK"
                     /locus_tag="VFC15_12095"
     CDS             complement(5503..5934)
                     /gene="rplK"
                     /locus_tag="VFC15_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZM10940.1"
                     /translation="MAKKVTGTVKLQIAAGKATPAPPVGPALGQAQINIMEFCKQFNA
                     RTNAKELDGLIIPVVVSVYTDRTFTFITKTPPASILLKRAAGVAKGSGVPNKDKVGKV
                     TEKQVLEIAKQKMPDLNAASVEAAVKSVKGTARSMGIDVTA"
     gene            complement(5969..6343)
                     /locus_tag="VFC15_12100"
     CDS             complement(5969..6343)
                     /locus_tag="VFC15_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10941.1"
                     /translation="MGTFSAKLRVWNPASPSRVEEMDVIVDTGAAYSWVSRTKLESMG
                     VLPVRKMQFRTIEGHTIERDLAPVFVATDGFTGGDNVVMAEAGDMEVLGSHTLETLGV
                     TVDPVNKKLVPTVALALAAKTN"
     gene            complement(6350..>6772)
                     /gene="nusG"
                     /locus_tag="VFC15_12105"
     CDS             complement(6350..>6772)
                     /gene="nusG"
                     /locus_tag="VFC15_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZM10942.1"
                     /translation="LIPTESVTEVRGGKKYTSERMFYPGYVLVEMDMDDNVWHVVKST
                     PRVTGFVGTGQQPTPLSDEEVQQIVYRVADSKEKPKLKVKFEKNESVRISEGPFASFT
                     GIVDEVNEDRETLKVMVTIFGRSTPVELEFGQVEKVIT"
BASE COUNT         1239 a         2140 c         1849 g         1544 t
ORIGIN      
        1 gtatcgcgcg actcgatctc gaattcctcg atgtggacgg aggtgtaata gtcgtccttc
       61 accatcttct cggaaaccag tatcgcgtcc tcaaagttgt atccgcgcca tgccatgaaa
      121 gcgaccagca cgttgcggcc cagtgccaac tcgcccatgt cggtgcaggg gccgtcggca
      181 atgacctgcc ccttggccac gcgcgttccc ttcttcacga tggggcgctg gttgatgcag
      241 gtgttctggt tcgagcgctt gaatttggtg agctgataaa tgtcgctgcc cacctcgcgc
      301 gaaagctgca tgggatggtg ctcgccctcg acacgcacga tgatgcgctc ggagtccact
      361 gaatcgatga tgccgctgcg gcgtgccagc accacggccc ctgagtcgcg agcggtaaca
      421 ccttccattc cggtaccgac gatcggcgct tgcgcgcgga gcaacggcac cgactggcgc
      481 tgcatgttcg cacccatcag ggcgcggttg gcgtcgtcgt gttcgaggaa aggaaccagc
      541 gaagcggcca ccgataccag ctgtttcggg ctgacgtcga tgtagtccac ttcattgcgg
      601 ttcactagca cgaagttgcc ggccttgcgg gcgttgacca gttccgcgac gatgcggccc
      661 ttgtcgtcaa tttccgcatt ggcctgcgca atcacgtgtc ggtcttcttc ccatgcggac
      721 aaatagaacg agaacggctc gtaatcgatc agcttcttct tgcggtcgcg cagctcattg
      781 ttgagtttga tgacctcggc tttctccaga tgatcgccga cgcgacggtc gctatctccc
      841 gcattgacca cgctgacaaa gtcgagaacg tgacccgcct tcaccttgcg atacggtgac
      901 tcgatgaagc cgtaatcgtt gatgcgggca taacaactca gcgacgaaat caggccgatg
      961 ttcggacctt ccggcgtctc aatcgggcag atacgtccgt agtgtgtcgg gtgaacgtcg
     1021 cggacttcga atcctgcgcg ttcgcgcgac agaccgcctg ggccaagcgc tgagagacgc
     1081 cgtttgtgcg tgatctccga cagcggattg gtctggtcca tgaactgcga caactgcgac
     1141 gatccaaaga actcgcggat cgcggccatc accggcttgg cattcaccag gtcgtgcggc
     1201 atcgccgtgg acatttcctg gtacacgctc atcttttcct tgatggcgcg ttccatgcga
     1261 accagtccga tgcggaactg gttttccagc aactcgccca ccgcgcgaac acggcggttg
     1321 ccaagatgat cgatgtcgtc caccgaaccg atgttcttgc gcagcttgag caggtaccga
     1381 atcgtgccgt agaagtcgtc gggctccagc gtacggtggt cgaggtgggt tgcgtcctcg
     1441 ctctcaaaca gtttgatgtt gaacttcagg cggcccaccc gcgagaagtc gtatttccgg
     1501 gaatcgaaga acattccgtg gaagagcgac gtcgcagtgt cgagtgtcgg cggatcgccc
     1561 ggacgcaact tgcggtagat ctcgatcagc gcttcctgcg gcgttttgac ggagtcgcgg
     1621 cgcagcgtct ggctgatcac cgtgcctacg tcgtcgcgct cggggaagaa caggtgcagt
     1681 tccgcaacgc ttgcctcgag catcttcgac agcttgtcgg tggtcagctc ggtgttggct
     1741 tcgagcatca cctcaccggt ggcggtatcg atcacgtcgg ccgcgacgaa tgcgccttcc
     1801 aggtccgtgg tgtcgatttc gatctcggaa accttgtgct tgaggatttc cttcatcacc
     1861 gaagccgtca ccttgcgtcc cgagtgcgcg atctcttcgc cactcttggt tgcgattttg
     1921 tgcgccagct tcatgcccag caggttggtt gggcggtcaa ctcccggctc cagcgtccag
     1981 taaatcttgt tgtcgcgcag agcgatgcga tcgatcgtgt aaaacgtccg gagaatgtcc
     2041 tcgtccgaac gcaggcccaa cgcgcgcagg aaaatgcttc ccaggaactt gcgcttgcgg
     2101 tcgatgcgaa catacaggat gttcttctgg tcgtattcaa actcgaccca cgaaccgcgg
     2161 tacggaatga tcttccccag gaagtacgtg cggttgttcg cgttctcgaa gaagacgcca
     2221 ggcgagcggt gcaactggct gacgatcacg cgctcagtcc cattgatgat gaacgtgccg
     2281 ttttgcgtca tcagcggaat gtcgccgaag aagacttcct gctccttcat gtcgcggacg
     2341 gagcggttgc cggtctccgg gtccttgtcg aaaatcgtga gccgcattgt gaccttgagc
     2401 ggcgcagagt aagtcatgcc acgctcctcg cattcagcca cgtcgtactt caattgcagt
     2461 cccaccggat cgccgcactt gttgcagaag tcgggcgtgt tggcgttgta cgtgccgcac
     2521 ttggtgcaca gcacgtcgcc cagatggaac ggatcggtct tcaccgtcga accgcaattg
     2581 cggcaggtgg tgcgcaggtg gttcagcccc ttgaggtggc cgcacttgca ctcccagttg
     2641 ccgatggcga actcgacaaa atcgagctgc gacacattgc ggaagtcgga gatgggaaac
     2701 acagactgga acaccgcctg caaacccgaa tcgtcgcgct cgctgggcag cgaatccatc
     2761 tgcagaaagc ggtcgtagga acgcttctgg acctcgatca ggttcgggat ctggatggtc
     2821 gccggaattt tggaaaagtc gaaacgattt ctattggcag ttttctgttg cgccatgtat
     2881 aaagctccta atcagtgccg gtcaggctcg ctcgcctagc cgcgcaagcc gcgattatgc
     2941 ggtggtgcga cgcggacatc tgcgccgtac ggtgacgggt gggaccgaaa gaaaaacgct
     3001 ctgcgggagt tttgcgcaaa gacacgagcg cgcccgcaga gacagaactc tccgcaagcg
     3061 ccaagtgagg cgttgagcaa tcccatgacc attcagtccg ggaacgcggc gagtcgaatc
     3121 gtgccgcgct aagttttaac ttccgctgca gtattttcag gccttgtcct cggctcgctc
     3181 gccgtcgcgt ctgagccgac gcgatgctgg ctgaacgtgc cgcctgtcat tacctgctga
     3241 gttgcccgga ttcgaatcgt gcttgaatcc gtcccaacga tgcccgggaa ttctgtggtc
     3301 cctgctgagg ggagccactt cgcttccgcg gaatcatcgc gcctctggac gcccgctcca
     3361 acgtcaagag gaacttgtgc cgccgtcgcg tggaccgctg acggcagccg cctgtaactc
     3421 cggtcagcgt gcccggagtg gaatcgcccc gttccgtcgc cagaacggga cggcgaacta
     3481 ctttacttct accgatgcgc ctgcatcgac aaacttcttc tggatggccg cggcttcgtc
     3541 tttcgtgatg ccttccttca cggtcttggg agcgccatcg accaggtcct tggcttcctt
     3601 tagaccgagg ctggtcactt cgcggaccgc cttgatcacg ttgatcttgt tggcgcccgc
     3661 tgcggtcagg atgacggcga attccgtctt ctcttccacc ggtgccgctg ctgccgcacc
     3721 cgcgcctgcg cccgctacca taacaggggc cgcagccgct gccgatacgc caagacgcgt
     3781 ttccagactc tttaccagtg ccgccgcatc aagcaacgac aggcctacga ttgtttcttc
     3841 caactgctgc agatccgcca tgtgtgtctc cacttgttcg ttattcgtcg ttatgatttc
     3901 gttaccagtt tccgaggctt ccagtgtgcc cgatcttgcg atcgccccgt atgccagact
     3961 cacgcgggaa aagcacgcct cacaacggat aactcaatct cttatggaag cactctcgtg
     4021 ctcccaaact cgtgtgttcg gtcgaagcct cgcgcctatg cgctggcggc ttcttcccct
     4081 gcaaacttgt tcttctcgac gccctggttg atgaccactg ccaggtcgcg gccaatcgca
     4141 ttgatcacgg tgaccaggcg ctgcgccgga gcgctcatca ggaacagcag cttcgagtag
     4201 atttcttcct tcgacggcat gctcgccagc gcgtcaatgt ccttgatcgc gatgacgcgg
     4261 ccatcgacga tgcccgcctt gaaggtgtac tccggatggt ccttggcgta cttgctcaga
     4321 accttggcca gcgcaaccgg atcgccgctg gtgtaggcaa tcgacgtaac accggccaat
     4381 cccttcactg cgtcttcgac cttggttccc ttggctgcgc gttccgctag cgtgtttttc
     4441 accacgcgat agctggcgcc agcgctgcgt actgccttgc ggagctcgta atctttctcc
     4501 accgtcagct tggtaaacgt gcctacgatt gcgtggctcg ccccctgaag gtcatggctc
     4561 agcttctcaa cctgttcgat tttcttcgct ttagtaaccg ccatcttcga ttccttttcc
     4621 cgccgcgctc atcgcgccag ccgattcttc aggctacccg tgcgctcaag gcgcaaatcc
     4681 cgggtggtta gctcttcgcc gccaattcca cggccgcaat gtcaatcgca acccccggcc
     4741 ccatcgtgga actcaggtag cacgccttga tgtatttacc cttcgcaacc gaaggcttgg
     4801 ccttgatcac gctggtaatt acagtagcgg cgttctccac caacttctgc ggatcgaacg
     4861 aaatcttgcc caccggcacg tgtaccagtg cggtcttgtc ggtacggaac tcgactttgc
     4921 ccgccttggt ttccttcacc gcggccgcga cgtcgaacgt caccgtgccc gtcttcggat
     4981 tcggcatcag cccacggggg cccagcacct tacccagacg tccaaccgac ttcatcatgt
     5041 cgggcgtgga aatcacggca tcgaaatcgg tccagttttc cttctggatc ttctccacca
     5101 tgtcttcacc gccgacgaag tccgcgcccg ccgcttcggc ttcgcgctgc ttctcgccgg
     5161 aagcgatgac caggaccttc ttggtcttgc ccagtccgtg cggcagaatc accgtgccgc
     5221 gcaccatctg gtccgcatgc ttgggatcca ctcccaggcg catcgtgagg tccaccgtct
     5281 cgtcgaactt ggcgaacttc accttctgga gaagaggaac cgcttcctgc agcgtatacg
     5341 ggtgattttc caccccggca cgcgccttct caacgttctt tcctgatttc ttcatgctgc
     5401 tccttgtctt ccaccgccgc cctctgttcc ggcgcggccg tggtgtttgc gaccgcaatc
     5461 tcgcgattgc gaataatgat gtcccacatt gtgggataaa ctttacgccg taacgtcaat
     5521 gcccatggag cgcgcagtgc ccttcacgct cttgaccgcc gcttccaccg aagctgcatt
     5581 caaatcaggc atcttctgct tggcgatttc cagcacctgc ttctccgtga ccttgccgac
     5641 cttgtcctta ttagggaccc ccgaaccctt cgccacgccc gcggcgcgct tcaacagaat
     5701 cgaagccggc ggagtcttgg tgatgaaggt gaaggtgcga tctgtgtaca cgctgaccac
     5761 caccggaatg atcaggccat ccagttcctt ggcgttggtc ctggcattga actgcttgca
     5821 aaactccatg atgttgatct gcgcctgacc gagcgccgga ccaaccggag gagccggcgt
     5881 cgccttgccc gccgcaatct gcaacttcac cgtacctgta actttttttg ccatagttgt
     5941 tcttctcaaa cgggccaacg cccgactgtt aattagtctt tgccgctaaa gccagtgcaa
     6001 cggtcgggac aagtttcttg ttcaccggat cgaccgtcac cccaagggtc tcgagcgtgt
     6061 gcgatcccag cacttccata tccccagcct ctgccatcac tacgttgtca ccaccggtaa
     6121 agccatcggt tgccacaaac acgggcgcca gatcgcgctc aatggtgtga ccttcgatcg
     6181 tacggaattg catcttacgt accggaagca ctcccatgga ttccagcttc gtacgcgaga
     6241 cccagctata cgccgcaccg gtatccacaa tgacgtccat ctcttccacg cgtgacggac
     6301 tggccgggtt ccacacccgc aacttcgctg aaaacgttcc catagctacc tacgtaataa
     6361 ccttttcgac ctgcccgaat tccagttcca ccggagtcga gcgcccaaaa atcgtgacca
     6421 tcaccttcaa agtctcgcgg tcctcgttca cctcgtccac aatgccggta aagctggcga
     6481 atgggccttc ggatatgcgg accgactcgt tcttctcgaa cttgaccttg agcttgggct
     6541 tttccttgct gtcggccacg cggtacacga tctgctgcac ttcttcgtcc gacagaggcg
     6601 tgggctgctg gcccgtgccc acaaatcccg tcacgcgtgg cgtggacttc accacgtgcc
     6661 acacgttgtc gtccatgtcc atctccacca gcacatagcc gggatagaac atgcgctcgc
     6721 tggtgtactt cttgccgccg cgaacttccg tgaccgactc agtcggaatc ag
//
LOCUS       DASRKK010000528         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_337896, whole genome shotgun sequence.
ACCESSION   DASRKK010000528 DASRKK010000000
VERSION     DASRKK010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337896"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..541)
                     /locus_tag="VFC15_12110"
     CDS             complement(233..541)
                     /locus_tag="VFC15_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10943.1"
                     /translation="MHYNKALYRHAEAELRDQALAMPPRFDSVQFCNGFSANHSQGYE
                     WLVRRYMNSPRAWDRPHAVQIAHREITHTLKNKYSDLVEKVGEAPNPHGGTMSLWRRL
                     "
     gene            complement(560..>2741)
                     /gene="leuS"
                     /locus_tag="VFC15_12115"
     CDS             complement(560..>2741)
                     /gene="leuS"
                     /locus_tag="VFC15_12115"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZM10944.1"
                     /translation="GFAYDWSKELATCLPEYYRWNQWFFLKFFEQGLAYRKKSKVNWC
                     PECATVLANEQVLPNGCCWRHENTPVEQRELEQWFLRTTKYADELLQGLDTLQGWPEK
                     VRTMQRNWIGRSEGTLVDFKLDGAAGPAGDKISVFTTRVDTIYGATSLQLAPEHPLVT
                     DLIANDPYLLAKVEELIAEQRKAKEVGDIGAIEKRGVSTERFALNPFNGEQVPIWVAN
                     YVLLDYGTGAIMSVPAHDERDYEFAKKYDLDIRVVILPRREGDAPDGEPNEPVLPFTE
                     MNSLVINSGEYTGMPCQEAIVKMSKLAEDQGFGKATVTYRLKDWGVSRQRYWGTPIPM
                     IYCPACGIVPVPEKDLPVLLPDNVDITLAGGSPLGKVPEFVKVNCPKCGGPARRETDT
                     MDTFVDSSWYFYRYTNPDLDTKPLDSDTVGYWFPIDQYIGGVEHAILHLIYSRFWTKV
                     MRDMGLVKNSEPAERLFTQGMVIKGGAKMSKSLGNVVSPDDMVGRYGADSTRMYTLFA
                     SPPDRDLDWQDAGVEGISRFLSRVYRFVARNARPNGTAPASRDGGQLSPAARQMQRKL
                     HQTIRRVTEDFGGRWHFNTSIAAIMELLNEMYSYEDSVQRGEPEAPPALVADAQRNLV
                     LMLAPFAPYLAHELWEMLDETSDLLRAPWPQYDAALAKEEEVEIAIQVNGKIRSRITI
                     PADAEEAQVRELALADEKVKAAIADKEVVKVLVVKGRLVNIAVK"
BASE COUNT          522 a          847 c          776 g          596 t
ORIGIN      
        1 aaaaaagact caaacaagta aactcggcat tttgcgcaac gacgattcaa taacttacga
       61 gtgacttttg agtcaagaaa cgctcgaaat tactactttg gttcattttt cagcagcctc
      121 tgaagccgtg ccctttcaaa gccttggtct tctcccagca gctcttctcg gctaagctct
      181 cccgtcccgc aaggcggact tgcataactc gggcacagcc ttgcagatga ggctagaggc
      241 gccgccacaa actcatcgtc ccgccgtgtg gatttggagc ttcaccgacc ttttccacca
      301 aatctgaata cttatttttc agagtgtggg tgatctcacg atgcgcaatt tgcaccgcat
      361 gtggccgatc ccacgcccga ggtgaattca tatagcgccg tactagccac tcataccctt
      421 ggctgtgatt cgcggaaaat ccattgcaga actgtacgct gtcgaagcga ggtggcattg
      481 ccaacgcttg atcgcgaagt tcggcctctg catgccgata caaagctttg ttgtaatgca
      541 tggaaccacc tccgccttct tatttcaccg caatgttcac cagccggccc ttcaccacca
      601 gcaccttcac gacctctttg tcggcgatcg cggctttgac tttttcgtcg gcgagcgcga
      661 gttcgcgcac ctgtgcctcc tcggcgtcgg cggggatggt gatgcggctg cgaatcttgc
      721 cattcacctg gatcgcgatc tcgacttcct cttccttcgc cagcgcagca tcgtactgcg
      781 gccacggtgc gcgcaacagg tcagaagttt cgtctagcat ctcccacaat tcgtgggcga
      841 gatacggcgc gaacggcgcc agcatcagta ccaggttgcg ctgcgcatcc gccaccagcg
      901 ccggcggggc ctccggctcg ccgcgctgaa cgctgtcttc gtacgagtac atctcgttca
      961 gcaattccat gattgccgca attgaggtgt tgaagtgcca ccgtccgccg aagtcttccg
     1021 tcacccgccg aatggtctgg tgcaatttgc gctgcatctg gcgggctgcg ggtgacaact
     1081 gtcctccatc gcgactggcc ggcgccgttc cgtttggccg cgcattgcgc gcgacgaacc
     1141 ggtacactcg cgacaggaac cggctgatgc cttccacgcc tgcatcctgc caatcgagat
     1201 cgcggtcggg cggcgaggcg aacagagtgt acatgcgcgt cgagtccgcg ccatagcgtc
     1261 cgaccatgtc gtcgggcgac accacgttgc ccaggctctt cgacatcttc gcgccgccct
     1321 tgatcaccat gccctgggtg aacaggcgct cggccggctc ggaatttttc accagtccca
     1381 tgtcgcgcat caccttggtc cagaagcgcg aatagatgag gtgcaggatg gcgtgctcga
     1441 ctccgccaat atattgatcg atgggaaacc aatatcccac agtgtcagag tcgagcggct
     1501 tggtgtcgag atcgggattg gtgtagcgat aaaagtacca ggacgaatcg acgaaggtgt
     1561 ccatggtgtc ggtctcgcgg cgcgccgggc caccgcactt cggacagttc accttgacga
     1621 actccggcac cttgccgagc ggcgatccac ccgcaagcgt gatgtccacg ttgtccggca
     1681 agagcacggg caagtccttc tcggggacgg gcacgatgcc acaggccggg caatagatca
     1741 tggggatcgg cgtgccccag tagcgttggc ggctgactcc ccaatccttc aagcgatagg
     1801 ttacggttgc cttgccaaag ccttgatctt cggcaagttt cgacatcttt acaatcgcct
     1861 cctggcaagg cattccggtg tactcgccgg aattgattac caggctattc atctccgtga
     1921 agggcaagac cggctcgttg ggctcaccat cgggtgcgtc tccttcgcgg cgcggcaaga
     1981 tcaccacgcg aatgtcgagg tcgtacttct tggcaaactc gtagtcgcgc tcgtcgtgcg
     2041 ccgggaccga catgatggcg ccggtgccgt agtcgagcag aacgtagttc gccacccaga
     2101 taggcacctg ctcgccgttg aacggattca gcgcaaagcg ctcggtggag acgccgcgct
     2161 tttcgatggc gccgatgtcg ccaacttcct tcgccttgcg ctgctcggcg atcagctctt
     2221 ccaccttggc cagcaggtat ggatcgttgg cgatcaggtc ggtcactagg ggatgctcag
     2281 gcgccaactg cagcgaggtc gcgccgtaga tcgtatccac gcgcgtggtg aagacggaaa
     2341 tcttgtcgcc agccggtccg gcggcgccgt cgagtttgaa gtcaaccagc gtgccttcgc
     2401 tgcggccgat ccagttgcgc tgcatggtgc gtaccttctc cggccagcct tgcagagtat
     2461 ctaaaccctg caacagttcg tcggcgtact tcgtggtgcg caggaaccac tgttccagct
     2521 cgcgctgctc gacgggagtg ttctcatgcc gccagcagca accgttgggc agcacctgct
     2581 cattggccaa cacggtcgcg cactcgggac accagttgac tttgctcttc ttgcggtacg
     2641 ccaggccttg ctcgaagaac ttgaggaaga accactggtt ccagcggtag tactccggca
     2701 ggcaagtggc gagttccttc gaccagtcgt aggcaaagcc g
//
LOCUS       DASRKK010000529         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_45226, whole genome shotgun sequence.
ACCESSION   DASRKK010000529 DASRKK010000000
VERSION     DASRKK010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45226"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1645
                     /locus_tag="VFC15_12120"
                     /pseudo
     CDS             266..1645
                     /locus_tag="VFC15_12120"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT          423 a          613 c          603 g          408 t
ORIGIN      
        1 gttttgttcg ccgcccgccg caatcatccc ctctccccaa ggaggtctgg attaacaagc
       61 caccactaaa ctaaagtcag taaattccct gtcggagtgt cccctgatcc gcacgtaatt
      121 ttctgaaatc gcgaaaagat cgcattccca gcgcattttc cccttgacat gttatagata
      181 gtgtatctat attatgtcta tgtcggcacg cattccaact caatataagt cgcccttcga
      241 aatcgacgcc cgacccttgg acgagatgtc cagcccccac gccggactgc tggccacctc
      301 ccgcgccttc cgctctctgg ccatcccggg tctcatcgat gcccatctcc agatcaagca
      361 gcgccagcgc ggttttgacg aggcccaata tatcgaatcg gtagtcctgc tgcaaacggc
      421 cggcggcgat tgccccgagg ataggcaact gctggggggc gatacctgcc tggaacgagg
      481 cttgggtttc aagctcccca aggtcggcgc ggtgcgaagt tttctcaatc gttttcacga
      541 tgaatccttg gaactgtccc gcccccagcg ggaggtgcag aagagtttca ttatgcccag
      601 cagtgcgccc gtggagggat tgcagtcggt gcaagccggc gtggtgcgcc ggattgccca
      661 gctctacgcc gagcagcaac agcccttgcg gaaggcgacg gtggaccagg atgcgacgat
      721 tattgagagt tacaaggagg cggcacgggc gcactatgag ggtgggcgcg gctaccagcc
      781 catggtggcg atctgggcgg aagccgatct ggtcttggcc gacgagtttc gcgatggcaa
      841 tgtgcccgcc caccaggagc cgctcacttg cgccaagctg gcctttgcgg cgttgccgga
      901 aaatatccag gaacgctatt ttcgcggcga ctccgcctgc cacgagaaca gcttgctcgg
      961 ctggctcaaa catccggacc gagcccagga gccggggggg cgcattggct ttgctgtcag
     1021 tgccaagagg agcgcggagt tggccgccgc cctggcccaa attccggacc aggagtggca
     1081 gacctatgat caggaagccg acggcacgtt gcggcaatgg gccgaggtcg attttgtccc
     1141 gggcgaacct ggggaaaaga aggacagcca acccttgcgc taggtcgggc tgcgcctgct
     1201 caagccgcaa ggttcgctct tcgcggatgg cacggaccga catcaccatg cggtgattac
     1261 caatttggaa ggggacgggg ccaagttgct caagtggcac cgcgcgaaag ccggcaccgt
     1321 cgagcacgtg catgatgagg tcaagaacga gttggggggc gggcacatgc ccagccaaca
     1381 ttttgccgtc aacgccgctt ggttcaagct ggcgttgtgg acctataata ttgccagcgc
     1441 gatcaagggg ctgtgccttt cgccggaaga acggaccgca cgctttaaga agtaccggct
     1501 cctgctggtg catctagcgg gccggatgag ccgcttccgc tgcaagctgc gcttgcgttt
     1561 ttgcgccagc caggaagcca tcgcccgcgt gcagaaagtc tgggccgtct tcgccctacc
     1621 cacgcaagca accgccttca catgaaggcg cccgcaaaaa cctaatcacc ctttcaactt
     1681 ccgaaaatcc tcacaggacg attttcggga ttttcccttc gcggttgagc tggcgtaccc
     1741 ctgcctcttc gtctccaaac agatccgcat tgagcgtagg gagccgttcc tgggttcaca
     1801 taatccttgc tgcaagcaac gcaccgacga agttgcgcgc agaaaatcct gccagcgtcg
     1861 ccgctgcggt tcttacgtgc ggatttggga ggacatccgt cttgacagtc cagccctgac
     1921 aggtgctgca ctggcagcgc atggccgggt cgttcacctc agcgcttttg caagctaacg
     1981 tggagacagg cgacggcgat tggctgcaac attcaatgag ggcaagagca tgaaaaaagt
     2041 atctaag
//
LOCUS       DASRKK010000530         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_729710, whole genome shotgun sequence.
ACCESSION   DASRKK010000530 DASRKK010000000
VERSION     DASRKK010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729710"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFC15_12125"
     CDS             complement(<1..321)
                     /locus_tag="VFC15_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10945.1"
                     /translation="MSTNSGVREAAVPSVFRNLSNSAPVQIIGLLLLFSMAAWYEAFH
                     LSALADSDIWWHLRTGLWISQNYGVPHNGLFSQHASLPWIDSSWGFDLLTAAAYRILG
                     LRSLP"
     gene            complement(358..2253)
                     /locus_tag="VFC15_12130"
     CDS             complement(358..2253)
                     /locus_tag="VFC15_12130"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM10946.1"
                     /translation="MKHKLLFVCAVLMLAAGTLLAQITGDVIGMHDLGPGSKSPITGA
                     RPGSCTYCHEPHSGAGGRTPLWNQTQTTATYTLYTSTTYHQKGAQPTLGADSNLCLSC
                     HDGTVAPGQTIVYGQIAMTGSMYPADVFGTNLQSSHPFSLVLPIKDTIDLIASLAGQG
                     KTGDPTGKVTLIDGNIECTSCHNPHVQAIDKLSLNFLVRDSSNAQMCLACHDPTRTMS
                     GQVNPLAGWATSAHAMATNKVAGQANLGSYGTVAQNACISCHVPHNAQGPMRLLRGPN
                     EQDCIACHNGSSNLNPAIPNVYAEFAKVGHPFPTGNNAHDAAEDVLLNNNRHATCADC
                     HNGHSSNQVTIFPPPPAIRVSQSGVAGISATDGISIVNPSVNQYENCLRCHGTSSGKV
                     ANPIFGYLPLRAVSAGDPLNVIPQFATTATSSHPVIHQRMSSLPQPSLLITMSDLSGG
                     TTHGRSMGNQIFCTDCHNSDDNREFGGVGPNGPHGSANKHILERDYEFSQAPGPGQLI
                     TNLYPNPDLSIKGPYAMCGKCHDLNNVVSNASWNHHGSHINTGFTCSVCHTAHGMGAR
                     SGTISGERLVNFDINVVAANGGMPVSYTRATNTCTLVCHNVAHNQDGSISQATQRNNG
                     TMLPKKR"
     gene            complement(2250..3353)
                     /locus_tag="VFC15_12135"
     CDS             complement(2250..3353)
                     /locus_tag="VFC15_12135"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZM10947.1"
                     /translation="MVQLLLTGARTRVPHFVSNVACVVLLLLTPVWASKPKDQPAQPA
                     PPDMVLEGGRKLSYERSFSNQREVKPKRGFWNRVLDFVAGEPEFRSLIRPYSIVVDSR
                     GRIIVTDPGAVGVHIFDFQQQKYKFLSRADKGKDPMLAPQCVAVDAQDNIYVTDSESG
                     KVFVFNSDGKYQRAIGSLKGGEGFFKRPTGIAVDSSAQRIYVTDTLRNKIFVLDMQGS
                     VVQSIGENGEGPGKFNFPTELRLDGQNLMVVDAMNFSVQVLDRSGAYKYAVGKIGDSP
                     GIMFRPKGIGLDSEGDLYVVDGLWGVVQVFNRDGDLLYYFGNRGTGAGEFQLPAGLFI
                     DHNDRIFVVDSFNRRVQVFHYYGVTKPAKGGSQ"
BASE COUNT          715 a         1011 c         1040 g          728 t
ORIGIN      
        1 cggcaaactg cgcaacccta atattctgta ggccgcagca gttaggagat cgaagcccca
       61 actggaatcg atccacggta acgacgcgtg ctgcgagaac aggccgttat gcgggacccc
      121 ataattctgc gatatccaga ggccggtacg gagatgccac cagatatcgg aatcggccaa
      181 cgcagaaagg tggaacgctt cataccaggc tgccatgctg aacaacaaca gcaacccgat
      241 tatctgcacg ggcgcgctgt tgctcagatt gcggaagacg ctgggaacag ccgcttcccg
      301 caccccggaa ttcgtggaca tagttgttcc gtcacccagc agcagccaac tgcggtctta
      361 ccgcttcttg ggaagcatgg tcccgttgtt cctctgcgtg gcttgtgaga tcgagccgtc
      421 ttggttgtga gcgacattgt ggcacaccag cgtgcaggtg ttcgtggcgc gggtgtagga
      481 gaccggcata ccaccattcg cagcgaccac gttgatgtcg aagttcacca gacgctcgcc
      541 ggagatggtc ccgcttctcg cgcccatgcc gtgggcggta tggcaaaccg agcaggtgaa
      601 gccggtgttg atgtgactgc cgtggtggtt ccagcttgca ttggagacga cgttgttaag
      661 gtcgtggcac ttgccgcaca tggcgtaagg acccttgatg cttaggtcag ggttcgggta
      721 caggttcgta atcaactgac cgggtccggg cgcctggctg aactcatagt ctcgttcgag
      781 gatgtgcttg ttggccgatc catgcggacc gttgggaccg acaccgccga actcgcgatt
      841 gtcgtcgctg ttgtggcaat cggtgcagaa aatctggtta cccatggaac gcccgtgagt
      901 ggtgccgccg ctcaaatccg acatcgtaat cagcaagctg ggctggggta gcgaactcat
      961 gcgctggtgg atcacgggat ggctcgaggt ggcagtggtg gcgaactgcg ggatgacgtt
     1021 cagaggatcg cccgccgaaa ccgccctcag cggcagatac ccgaagatgg gattggcaac
     1081 ttttcccgaa cttgtaccgt ggcaacgcag acaattttcg tattgattga ccgaaggatt
     1141 aacgatggat atgccgtccg tggcactgat tccggcaacc ccactctgcg acacgcgaat
     1201 ggccggaggc ggcgggaaga tagtcacctg gttggacgaa tgtccgttgt ggcagtccgc
     1261 gcacgtggca tgccggttgt tgttcaacaa aacgtcctcg gcagcgtcgt gtgcattgtt
     1321 acccgtagga aacggatgtc ccaccttggc aaactcggcg tagacatttg ggatcgcagg
     1381 gttgagattc gaactgccgt tgtggcaagc aatgcagtcc tgctcattcg gcccgcgcag
     1441 caggcgcatc ggcccttgcg cgttgtgtgg cacatggcag gagatacacg cgttttgcgc
     1501 caccgtgcca tagctgccaa ggttcgcctg cccggctact ttgttggttg ccatggcgtg
     1561 tgcgctggtt gcccatcccg cgagaggatt gacctgcccg gacatggtgc gggtgggatc
     1621 atggcaggca agacacatct gcgcattcga actgtcgcgg accaggaagt tcagcgacaa
     1681 cttatcgatg gcctgaacat gagggttatg acaggaagtg cactcaatgt taccgtcgat
     1741 cagcgtcact tttcccgtcg gatcgcctgt cttgccctgc ccagccaacg acgctatcaa
     1801 gtcgatcgta tctttgatgg gcagtaccag gctgaaggga tgcgaactct ggaggttggt
     1861 cccgaagacg tcggcgggat acatggatcc agtcattgcg atctggccat agacgatggt
     1921 ctggccgggc gcgacggttc cgtcgtggca gctcaaacac aagttgctat cggcgcccaa
     1981 ggtaggttga gcacctttct ggtgataggt cgtactggtg tagagcgtat aggttgccgt
     2041 tgtctgcgtc tggttccaaa gcggcgttct gcctccagcc ccggagtgcg gctcatggca
     2101 ataggtgcag gaaccaggcc gggcgccggt aatcggcgac ttactgcccg gccccaggtc
     2161 atgcatgccg attacatctc cagtgatctg cgccagcagg gtgcccgcag ccaacatcag
     2221 gaccgcgcag acaaatagca acttgtgctt cactgggacc ctcccttagc cggcttggtc
     2281 acgccgtagt aatggaaaac ctggacccgt ctattgaagg aatccaccac aaaaatccgg
     2341 tcgttgtggt cgataaacag tccggccggc agctggaatt cgcccgcccc ggtgccgcga
     2401 ttgccgaagt agtacagcag atcgccgtcg cggttaaaca cctgcactac gccccacaac
     2461 ccgtccacca cgtagagatc gccctcggaa tcgagcccaa tccccttagg acggaacatg
     2521 atcccggggc tgtcgccgat cttgcctacc gcgtacttat aggctccgga ccggtccagc
     2581 acctgcacgc tgaagttcat ggcgtccacc accatcaggt tctggccgtc cagccgcagc
     2641 tcggtcggaa aattgaactt gccggggcct tcgccgtttt ccccgatgct ctgcaccacg
     2701 ctgccctgca tgtccagaac aaatatcttg ttgcgcagcg tgtcggtcac ataaatccgc
     2761 tgcgccgagg aatcgacggc aatgccggta ggacgcttga agaaaccttc gccgcctttc
     2821 aggctcccga tcgcccgctg atacttcccg tcagagttga agacgaacac cttgcccgac
     2881 tccgaatcgg tcacgtagat gttgtcctgg gcatccacgg cgacgcactg cggcgccagc
     2941 atcgggtcct tccctttgtc agcgcgagac aggaacttgt atttctgctg ctggaaatcg
     3001 aagatgtgga ccccgaccgc tcccgggtcg gtcacgatga tgcgccctcg ggaatcgaca
     3061 acgatgctgt agggacggat aaggctgcgg aactccggct cgccggcaac gaagtccagc
     3121 acccggttcc agaaaccgcg cttcggcttc acttcgcgct ggttgctaaa gctgcgttcg
     3181 tagctgagct tgcggccgcc ctccagcacc atatccggag gtgcaggttg tgcaggctgg
     3241 tctttgggct tggacgccca gaccggagtc agcagaagaa gaacgacaca ggcgacgttt
     3301 gagacgaaat gtgggactct cgtgcgcgct ccggttagaa gaagttgaac cacctagtca
     3361 ctccaatgaa gtacgtggac actttggccg gcgcaaggtc ccccacgctg aagccctgcg
     3421 aaatatgagc gtatcccccc gttaagtaca ttttacggaa ttggtattgc aacagcgcat
     3481 tcgctccctc agat
//
LOCUS       DASRKK010000531         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_729758, whole genome shotgun sequence.
ACCESSION   DASRKK010000531 DASRKK010000000
VERSION     DASRKK010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729758"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..513)
                     /locus_tag="VFC15_12140"
                     /pseudo
     CDS             complement(1..513)
                     /locus_tag="VFC15_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            684..2330
                     /locus_tag="VFC15_12145"
     CDS             684..2330
                     /locus_tag="VFC15_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM10948.1"
                     /translation="MFIRTQSNGSRTYLLIVDNERVAGKVTQRVLFRLGRLDQLLANG
                     QLDSLIQSLGRFSEKLAVLGAHARGDSIATRSARIGPALIFERLWQACSIDKVLTSLL
                     KGRRFEFSVERAIFLTVLHRLFAPGSDRAAEKWKDDYAIEGVADLDLHHLYRAMAWLG
                     EVLPNAQQDGATPFSPRTNKDLIEEALFARRRDLFSDLDIVFFDTTSIYFEGQGGETI
                     GQHGHSKDHRPDLKQMVVGMVLDRNGNPVCSELWPGNTADVKSLVPIVERLQSRFGIG
                     SVSIVADRGMISAETMAEVELRKWSYILGVRMRSSTEAKAVVARAGRYAEVQPNSDKR
                     DDPSPLKVKEVWVEDARRYVVCLNEDQATKDRHDREAVVASLRDALSKGDKSLVGNKG
                     YRKYLRAGGKQFAVDEDKIKEEARYDGKWVLTTNMDLPPAEVALKYKQLWMVEDVFRS
                     MKSLLDTRPIYHKCDETIRGHVFCSFLALLLRKELEDRLVRKEWKLEWADVIRDLGNI
                     VEMEVAISGKGYVFRGQTPGVAGKVFQACGVALPPTLRAC"
     gene            complement(2422..>2789)
                     /locus_tag="VFC15_12150"
     CDS             complement(2422..>2789)
                     /locus_tag="VFC15_12150"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10949.1"
                     /translation="EDDPGNYDLSFWLDYFQKIHADAACLSAGGVVAFYPTEIPLHYR
                     SRWLGTMDPFGDIVAGCRKLGMNVIARTDAHACHEDVYDAHPDWIAVDVHGNKRRHPS
                     DDQYWITCALGPYNSSSTP"
BASE COUNT          633 a          737 c          839 g          580 t
ORIGIN      
        1 cctatcgacc gaacgcgaat agcgccgttg gaggagcacg ttggccttgg tacgcaccac
       61 aaagaaggcc gacgacaagg taaagcgata gagacgctca aagtcgatgt aaccgcgatc
      121 catcacgtag aaggcgccgg cttcgatgac gatttcgtcg agcacgttga cgtcatgcac
      181 cttgccgttg gtgatgtgaa tgaaggtggg gatggagcca tgcagatcga gcagtgtgtg
      241 cattttgaca gcggccttgt gcttgcggaa tctggcccag ggaaaaagcg ccagacaaag
      301 atcgatggtc gttgagtcca gtgcgtacag atcgcgcaca tccgcgatgc cggtcgcgtc
      361 gttggcataa agcttgatcg ccgtgcgaat taacgcctgc gcaaagtcgg caaagatgcg
      421 ccaatcgcgg ttctcgttgg cgtcggccaa tgtggaacgc gccacggcgg agccaatgcc
      481 catgtgatac aacttagggc caatcgctcc cagactcatc ttctacagtt tgggcatgcc
      541 gagtgcccgt aagctgttga tttcattgag agcggttgtg gaatacttca aaatgtagtg
      601 tcacgtcata tagtcatata tgccatgttg attgtttatt tatctaatat gtagtgttac
      661 tatgagggcg caggagtggc gccgtgttta ttcgcacaca gtcgaatggt tcccgaacct
      721 atctgctcat tgtcgataac gagcgggtgg ctggcaaagt gacgcagcgc gtcctgtttc
      781 gccttggccg gctcgatcag ttgctggcca acggtcagct tgactcgctg atccagtcgc
      841 tgggccggtt ctcagagaag ttggctgtac taggcgccca tgcgcggggc gacagcatcg
      901 ctacccgtag cgccaggatc gggccggccc tgatctttga gcggctctgg caggcctgct
      961 cgatcgacaa ggtcctgacc agcttactca aaggccggcg ctttgagttc tccgttgaac
     1021 gagccatctt cctcaccgtt ttgcatcggc tctttgcacc tggaagcgac cgtgccgccg
     1081 agaagtggaa ggacgactac gcgatcgaag gagtcgccga tctggacttg catcaccttt
     1141 accgggccat ggcctggctg ggagaggttc ttcccaatgc ccagcaggac ggagccacgc
     1201 cattttctcc ccgcaccaac aaggacctga tcgaggaagc actctttgct cggcgtcgcg
     1261 acctgttctc cgatttggac atcgtgtttt ttgacaccac atcgatctac ttcgagggcc
     1321 agggaggcga gaccatcggc cagcacggac actcgaaaga tcatcgtccc gacctgaagc
     1381 agatggtggt gggcatggtg ctggaccgga acggcaatcc ggtgtgcagc gaactctggc
     1441 cgggcaacac agccgacgtg aagagtctgg ttccgatcgt agaacggttg cagagccgat
     1501 tcggaatcgg atcggtgtcg atcgttgccg accgagggat gatcagtgcc gagaccatgg
     1561 cggaagtgga gttacgcaag tggagttaca tcctgggagt gcgcatgcgc agttctacgg
     1621 aagccaaggc ggtggtggcg cgcgcaggcc gctacgcgga agtacagcca aacagcgaca
     1681 agcgggacga tccgtcaccg ctgaaggtga aagaagtctg ggtcgaggat gcgcgccggt
     1741 atgtggtgtg cctgaacgag gatcaagcta ccaaggatcg ccacgaccgc gaagcggtgg
     1801 tggcttcgct gcgcgacgcg ttgagcaagg gggacaagtc gctggtgggc aacaaggggt
     1861 atcgcaaata cctgcgcgca ggaggaaagc agttcgccgt tgacgaggac aagatcaagg
     1921 aagaagcgcg ctacgacggc aagtgggtgt tgacgaccaa catggatctt ccccccgcag
     1981 aggtggcgct gaagtacaag caactgtgga tggtggagga tgtcttccgc tccatgaaat
     2041 ctctgctgga tacccggccc atctatcaca agtgcgacga aacgattcgc gggcacgtgt
     2101 tttgctcctt cctcgcgctg ctgttacgca aggaactgga ggaccgttta gtacgcaagg
     2161 agtggaaact ggaatgggcc gacgtgatcc gggatctggg aaacatcgtt gagatggaag
     2221 tcgcaatcag cggaaagggc tacgtttttc gcggccagac tcccggcgtg gcgggcaaag
     2281 tatttcaagc ctgtggcgtt gccctgccac ccacgctgcg tgcatgctga acccatcttc
     2341 aattcgacgc agatggagag aaaacgtgtc actacgccat tttcaaaact gcaacatgct
     2401 gataatcaat cacttccgaa actatggtgt agaagatgag ttatacggac ccaaggcgca
     2461 agttatccaa tattgatcat cggaagggtg ccggcgcttg ttgccatgca catcgacggc
     2521 gatccaatca ggatgcgcat cgtagacgtc ctcgtgacaa gcgtgggcat ccgttcgtgc
     2581 aatcacgttc atcccgagct ttcgacatcc cgccacgatg tctccgaacg gatccatcgt
     2641 cccaagccat ctgcttcgat aatggagtgg aatctccgtc ggatagaaag cgacaacgcc
     2701 tcccgcactc agacaggccg catcggcatg aatcttttga aaataatcga gccagaatga
     2761 aaggtcgtag ttccccggat cgtcttcaa
//
LOCUS       DASRKK010000532         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_827090, whole genome shotgun sequence.
ACCESSION   DASRKK010000532 DASRKK010000000
VERSION     DASRKK010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827090"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFC15_12155"
     CDS             complement(<1..1151)
                     /locus_tag="VFC15_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM10950.1"
                     /translation="MATANQTLSYGATRRGKEAEFRVWAPLPNEITLRLTRVGAEPQD
                     IPMPRDGEDFVAVAAAATGDRYAYILEDSPPIPDPVSRFLPEGVHGPSEIVDPSAFHW
                     NADGWRGLALRDYVIYELHIGSFTPEGTFDAAIAKLEYLKDLGITVVEVMPVSAFPGV
                     RDWGYDGVAPYAVQASYGGPEAFKRFIDAAHRHELAVILDVVYNHLGPEGNYLRRFGP
                     YFTAQHTTPWGEAVNYDTAGCEHVRRYVVENALYWIREYRLDGLRLDAVQTIKDDSPK
                     HILAEIQERVQALARELGRSVNVIAETDENDSRYTGAAEDGGYGLDAVWSDDFHHAVH
                     AFFTGEREGYYQGFGEPDQIARALREGYVYQGQHYPFWNAPRGTSAKDVP"
     gene            1222..1800
                     /locus_tag="VFC15_12160"
     CDS             1222..1800
                     /locus_tag="VFC15_12160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZM10951.1"
                     /translation="MPLILASASPRRSELLRNAAIPFTVEPAHVPEQPAAHEKPVDYA
                     QRLARDKARAVFARHPQSAVLGADTVVVVDQHLLEKPTDQADAARMLRLLSGRTHQVI
                     TGVCLVAPHFEQTEAEITQVVFSPLSEREIAAYIDSGEPMDKAGAYAIQGMASRWVER
                     IEGCYFNVVGLPVPRVYRMLRAAEATTGRALP"
BASE COUNT          386 a          624 c          603 g          388 t
ORIGIN      
        1 ggcacgtcct tcgcgctggt acctcgcgga gcattccaga aaggataatg ctggccttgg
       61 taaacgtatc cctcgcgcag tgcgcgggca atctgatcgg gctcaccaaa gccctggtaa
      121 tagccctctc gctcaccggt gaagaacgca tgcacggcat gatgaaagtc gtcgctccat
      181 accgcgtcca gtccatagcc gccatcttcc gccgctcccg tgtaacgcga atcgttttcg
      241 tcggtctcgg cgatcacgtt gaccgagcgt cccaattcgc gggccagcgc ctgcacccgc
      301 tcctggatct cggcgaggat gtgcttcggc gaatcgtctt tgatggtctg caccgcatcc
      361 agacgcaagc cgtcgaggcg atattcgcga atccagtaca gcgcgttctc cactacgtaa
      421 cgacgcacat gctcgcagcc tgccgtgtcg tagttcacag cctctcccca tggcgtggtg
      481 tgttgcgccg tgaagtaggg accgaacctg cgcaggtaat tgccttccgg gccgagatgg
      541 ttgtaaacca cgtcgagaat caccgccagc tcgtgccggt gggcggcatc gataaaccgt
      601 ttaaacgcct cgggaccacc atagctcgcc tgcacggcat agggcgccac gccgtcgtat
      661 ccccaatcgc gcacgcccgg gaaagccgat accggcatca cctcgacaac cgtaatcccc
      721 aagtccttca ggtattcgag cttcgcaatg gcggcgtcga acgtgccttc cggcgtgaag
      781 ctcccaatgt gcagttcata aatgacgtaa tcgcggaggg cgagcccacg ccatccatcg
      841 gcgttccagt ggaacgccga tggatcgacg atctcggatg ggccatgcac accctcgggc
      901 agaaagcgtg aaacggggtc gggaatcggc ggactgtcct cgagaatata ggcgtaccga
      961 tcgccggtag ccgccgcagc gacggcaacg aaatcttcac catcgcgcgg catgggaatg
     1021 tcttgcggct ccgcgccgac acgcgtcagg cgaagcgtga tctcgtttgg gagaggcgcc
     1081 cacacgcgaa attcggcttc ctttccgcgc cgcgtagcgc cgtaggaaag agtctgattt
     1141 gcggtggcca cctaatatca gacgtgcgta ggtggcgatg ggatgctttc gatcgcgctc
     1201 gctggataga atcaggatga catgcctctg attctggctt ccgcttcccc acgccgctcc
     1261 gagctattgc gtaacgcggc gatcccgttc acggttgaac cagcccacgt tcccgagcag
     1321 cccgctgccc acgagaagcc cgtggactat gcgcaacggc tggcccgcga taaggcgcgc
     1381 gcggtgttcg ctcgccatcc gcaaagtgcg gtgctgggcg ctgacaccgt cgtcgttgtc
     1441 gaccagcatc tgctggagaa acccaccgac caggctgatg cagcgcgcat gttgcgtctg
     1501 ctttccggac gaactcatca ggtgatcacg ggagtatgcc tggtagcgcc ccacttcgag
     1561 cagaccgaag ccgagatcac acaagtggtc ttcagtccgc tctcggagcg cgagatcgct
     1621 gcctacatcg acagcggcga gcccatggac aaagcgggag cgtacgccat ccagggtatg
     1681 gcctcacgct gggtggaacg catcgagggt tgctacttca acgtggtggg acttcccgtg
     1741 ccaagggtgt atcgcatgtt gcgcgccgcc gaagccacta ccggtcgggc gctaccgtga
     1801 cggatttgca ggagggaagg cgcagtgagg gatgatcggg aaatccagag tcccgggcac
     1861 accgctgaac tcggcacaat ctcgtaacta cgacttcttg tcttcagtcg ggttcgggtt
     1921 cgggggcggg gttgtcgtgg cgttatgctc gttcatcgac gacttgaagt tgcgaatgcc
     1981 ttcgcccaag cctttgccca g
//
LOCUS       DASRKK010000533         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_436280, whole genome shotgun sequence.
ACCESSION   DASRKK010000533 DASRKK010000000
VERSION     DASRKK010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436280"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            760..1494
                     /gene="pal"
                     /locus_tag="VFC15_12165"
     CDS             760..1494
                     /gene="pal"
                     /locus_tag="VFC15_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZM10952.1"
                     /translation="MSVRSFRLFAVIAAASVTLLVGCAKKTHVATTVPPLPPPPPAPT
                     ASLAASPDTVERGQVVRLLWDTSNANEISIVGVGTVAARGTTSLIPQSSTTYVLTAKG
                     PGGSKEATARVTVTVPPVATSSAGENDEQLFSQNVKDVFFPYDKYSVPQNEQSTVKQD
                     AQFLAAHPNYKLLISGYCDDRGSEEYNLALGENRADAVRNQLEQLGIGASRIRTISYG
                     KEKPFCSQETEACWQLNRRAHFSMQP"
     gene            1724..>2068
                     /locus_tag="VFC15_12170"
     CDS             1724..>2068
                     /locus_tag="VFC15_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZM10953.1"
                     /translation="MMRSLLIMLLLVATVAANGVAGTIEGKVSPGKSVVYVDSIGSKT
                     FPAPTQTVLIEHKGMMFSPHTLVIQQGTTVEFLNQDNMQHNVFWPSISGNKKLGHNLG
                     TWPKGEKRGFKFD"
BASE COUNT          462 a          591 c          565 g          450 t
ORIGIN      
        1 gcctgccttg cgaagtcccg cgcgagtact tctatcgcct gccgcgagct gccgacagag
       61 ttgatcatcc cactttgtaa gaggtcttgc gtatcccggc cagcgcttcg ccctctgagc
      121 gtcgcacgga gccggcttac atggattatg gatgtaacaa ttttgccgct acatagcgaa
      181 ggaggactgc ccctgcgcta gatttggccg ctccgaacct cacgacgatc gacgtttctg
      241 cttctaccga tcgttcaacg ctctgtgagg gcgcaaggtg atgatgggcg gatgcgcagc
      301 cgacatattt ggaaacggat tcccgattgg tccatcgacg gagagtcttt ggactggaac
      361 agcaactctt agaaagaaac cgggttgaac ccggtgtagg acttccgata ggctatatcg
      421 aaacatgttg ccacggtgcc gaaataccgc ccttttggtt gccttgggag tactgttcgt
      481 cgccctattt ccgcttggag cgggtccatt caccgttacc catggtcctc tcacggcgtt
      541 gcgggcctta gcctttgcgc ttcttctctt cgcagcgatt tcgttgttgc tgacggtgac
      601 ggtcgccgac cctggcatgt acataaggct aagaatccaa gtcgtcggcg ttgcggtatt
      661 cgatgcggca cccgttccaa gtctgcgctg ctaatactct gccctccaag tttcttcgcg
      721 attctgttgt catcgatcac ttcaattttg gaggagcata tgtccgtgcg ttcatttcgc
      781 ttatttgccg tcattgcggc agcttccgtc acgctactcg taggctgcgc caagaagact
      841 cacgtcgcca ctacggttcc gccactgcca ccgccgccac cagcgccaac cgccagcctc
      901 gccgcaagtc cagacacggt cgagcgcggt caagtggtca gactcttgtg ggatacgagc
      961 aacgccaatg aaatctctat cgtcggggtc ggaacggttg ccgcgagagg caccacatcc
     1021 ctaatccctc aatcgtcaac gacctacgtg ttaacagcca agggtccggg agggtcgaaa
     1081 gaagccacgg cgcgcgtaac ggttaccgta ccaccagttg caacgagttc tgcgggagag
     1141 aacgacgagc aactgttttc gcaaaacgtg aaggacgtct tctttcccta cgacaaatac
     1201 tccgttcctc agaacgagca atcgaccgta aagcaggacg cacaattcct ggctgctcat
     1261 cccaactaca aactgttgat ttccggctat tgcgatgatc ggggctctga ggaatacaac
     1321 ttggccctgg gagagaatcg tgctgacgcg gtgcgcaatc aactggagca attgggcatc
     1381 ggcgcaagcc gaatccgaac cattagctat ggcaaggaaa aaccgttctg ttcccaagag
     1441 acggaagcct gctggcagct caatcgccgg gcgcatttct ccatgcagcc atgaactcgg
     1501 tgaccccagg ttgggcccga gatgctaatt ggacgcacaa attgcagtgt gacttcatgg
     1561 tcggcagggg ccggttgttc ccacatgccg tcgccctcgg cccgaacgga agcatctgct
     1621 gcagccggtc ggctgcactc acgagggggt catggtcgac gtcacactgc aaaatacttg
     1681 cggtacgaac ctgagagggc acaaacccaa gaaaggaagt ttcatgatgc gcagtcttct
     1741 gatcatgctg ttactggttg ccaccgtcgc agccaatggt gtcgccggca caattgaagg
     1801 caaagtatcg ccgggaaagt cggtcgtgta tgtggacagc atcggaagca agacattccc
     1861 agcccccacg cagacagtgc tgatcgaaca taagggtatg atgttcagcc cgcacacctt
     1921 ggtgatacag caagggacga cggttgagtt cctgaaccag gacaacatgc aacataacgt
     1981 cttctggcct tcgatcagcg gcaacaaaaa gctaggacac aacctcggca cctggccgaa
     2041 gggtgaaaag cgcgggttca agtttgac
//
LOCUS       DASRKK010000534         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_239881, whole genome shotgun sequence.
ACCESSION   DASRKK010000534 DASRKK010000000
VERSION     DASRKK010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239881"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFC15_12175"
     CDS             <1..982
                     /locus_tag="VFC15_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10954.1"
                     /translation="NATVDDITEAYIQAWKLGLKAVAVYRDGSKKAQPLSSMGSATAK
                     SKKGDALAAPVEVEDEAAAPPRAMRHRLPDERLSITHKFSVGGHEGYLTVGLYKDGMP
                     GELFITMAKEGSTVSGLMDSFACATSLGLQHGVPLKLMCEKFSHTRFEPSGWSHNQDI
                     GYAKSIMDYIFRWLQLRFLTGQQQALFDGLRPKYINAPPPGTPDENGYGTPGAGAGTP
                     PSLLSSRGEAEGSAFSSSVILSERSESKDPYSAKDPSQLVIPSGAPEGRGVEGPAFRG
                     GATGHYHASDALQDLIDMGDSPSCPVCGAIMTRNGSCYRCMSCGSTSGCS"
     gene            1288..1977
                     /locus_tag="VFC15_12180"
     CDS             1288..1977
                     /locus_tag="VFC15_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10955.1"
                     /translation="MSLGGGTKTNPVFINDKLEKQLPNQASAFLLCEECEKRLRENGE
                     TWVLEHCYRGRASFALHDILSKYSPYARIDDFMGFDVATVRDIDSNKLSYFAASVFWR
                     SAVHDWYLGEIHLGSISLGQYEESFRKYLLSEAALPENAAITVCVAESKECLPVALFP
                     ASRKNLLYHHHWFRIPGIAFHLYVGNGMDQALRSGCLVRSGVLYMSDLIDQKVVEDLG
                     RAITLRKARTY"
     gene            2020..2406
                     /locus_tag="VFC15_12185"
     CDS             2020..2406
                     /locus_tag="VFC15_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10956.1"
                     /translation="MAMNFFGAWAVGTIVGLYKAFILMHLWNWFVAPVFHLQEISFLL
                     MLGLLWTISLLTDRADTGEEKRWKVMHLILDKCVPEHRRDELTEELKAETDSVWFETG
                     MTMFASCVGYTATLALGWFVHILVGS"
     gene            2451..2741
                     /locus_tag="VFC15_12190"
     CDS             2451..2741
                     /locus_tag="VFC15_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10957.1"
                     /translation="MRNKHLLFVLVVLVTLCLTACVAGTNHLKDTSNADGDVAGFWLG
                     LWQGFIVFFTFVVSLFKDNVNIYEVHNNGGWYNFGFLLGVITFFGGAGSQSR"
BASE COUNT          628 a          805 c          846 g          642 t
ORIGIN      
        1 gaatgccacc gtggacgaca tcacggaagc ctacatccag gcgtggaagc tggggctgaa
       61 ggcggtcgcc gtctatcgcg acggatcgaa gaaggcgcag ccgctcagct ccatgggatc
      121 ggccaccgcc aaatcgaaga agggcgacgc gctcgctgcc cctgtcgagg tagaagacga
      181 ggccgccgcg ccgccgcgtg ccatgcgcca ccgcctgccc gacgagcgcc tgtccatcac
      241 ccacaagttc agcgtgggcg gacacgaagg ctatctcacc gtgggccttt acaaggacgg
      301 catgccgggt gagctgttca tcaccatggc caaggaaggc tcgaccgtca gcggcctgat
      361 ggacagtttc gcctgcgcca cgtcgctggg cttgcagcac ggcgtgccgc tcaagctgat
      421 gtgcgaaaaa ttctcccaca cccgcttcga gcccagcggc tggagccata accaggacat
      481 cggctatgcc aagtccatca tggattacat cttccgctgg ctacagttgc gcttcctgac
      541 cggccagcag caggcgctgt tcgacggcct gcgtcccaag tacatcaacg ctccaccacc
      601 cggcacgcca gacgagaacg gctatggcac gcctggagca ggcgcgggca cgccgccttc
      661 gttgttgtca tcacgaggcg aagccgaggg atctgctttt tcttcctctg tcatcctgag
      721 cgagcggagc gagtcgaagg acccctatag cgcgaaggac ccttcacagc ttgtcatccc
      781 gagcggagcg cccgaagggc gcggagtgga ggggcctgct tttcgcggtg gcgcgaccgg
      841 ccactatcac gcctccgacg cgctgcagga cctgattgac atgggagact ctcccagttg
      901 ccccgtctgc ggcgccatca tgacccgcaa cggaagctgc tacaggtgca tgagttgtgg
      961 cagcaccagc ggatgtagtt agagggtggt gcacgggtag tgcggcgctt cagcgccgcg
     1021 tcccacgccc ctttttgatg ttgtcatccc gagcggaggc gggtcccctc cggcgacccg
     1081 ccgaagtcgg gggacctgct gttaaggcca actgcgtagg ccgctcgcga cggcaccctt
     1141 cttatgcgag ttcaaccgcg attgcttcgc atccggacca ggtagatggg tcaacgcccc
     1201 actagaacgt gcgcactttg ccatagactc cgactacttt gtgacagtca ctttctgcct
     1261 aaagcgttat ataggtacat acgtcgaatg tcgctcggcg gtggcaccaa gaccaatccg
     1321 gtattcataa atgacaagct agaaaagcag ctgccgaacc aagcgtcggc gttcctactc
     1381 tgcgaagaat gtgagaaacg cttacgcgag aacggggaga catgggtact ggagcactgc
     1441 tatcgcggac gcgcgagctt cgccctgcac gatatcttgt cgaagtactc tccatacgcg
     1501 cgaatagatg atttcatggg gttcgatgtc gccacagtga gagatatcga ttcgaataaa
     1561 ttgtcgtatt ttgcagcaag cgtattctgg cgttccgcag tccatgactg gtatctgggg
     1621 gagatacacc ttggctctat atcccttggg caatatgaag aatcattccg gaaatatctt
     1681 cttagcgaag ctgctctgcc cgaaaatgca gcgatcaccg tatgtgtcgc agaaagcaaa
     1741 gaatgcctgc cggttgcgct ttttcccgct tccaggaaga acctgcttta tcatcatcac
     1801 tggtttcgca tcccaggcat cgcctttcac ctttacgttg gtaatgggat ggaccaagcc
     1861 ttgcgaagcg gctgcctggt gcgatctggg gttctttaca tgtcggacct cattgaccaa
     1921 aaggtcgtcg aggacctcgg acgcgcaatc actctacgaa aagcccggac ttattagcgg
     1981 gaaagtgcgt cccgtgaaac tactgagaag gaggccacta tggccatgaa tttttttggg
     2041 gcttgggcgg tcggaacaat tgtaggattg tacaaggctt tcatactcat gcatttgtgg
     2101 aactggtttg ttgcgccggt gttccacctg caagagattt cattcctatt gatgctggga
     2161 ttgctctgga caatcagcct gcttactgat cgtgcggaca ctggcgagga gaagagatgg
     2221 aaggttatgc accttatctt ggacaagtgc gtgcctgaac acagaaggga cgagctaacc
     2281 gaagaattga aagcagaaac tgatagtgta tggtttgaga caggcatgac aatgtttgcg
     2341 tcctgtgtgg gatacacggc aactctagcc ttaggatggt ttgttcacat attggtcggt
     2401 tcatgacgcg ggcgagcggc ccacccacca cctgaagaca tgaggacgag atgcgaaaca
     2461 agcacctgct attcgttttg gttgtattgg taacactctg ccttacggcg tgtgtggcgg
     2521 ggacaaatca cctgaaagat acctctaacg cggatggtga cgttgcagga ttttggctgg
     2581 gtctctggca agggttcatt gttttcttta cttttgtcgt gtcgcttttc aaggacaacg
     2641 tcaacattta tgaggtgcac aataacggag gatggtataa cttcggtttt ttgctgggag
     2701 tgatcacctt ctttggcggt gccggatcac agagtcggta gggcgggtga cggcgggtgg
     2761 cccaccctaa catctgacct tcccaaacct ggggtgcccc attcaacctt cttttggttg
     2821 ggtggggcat ctcgcatcta ccaacgaaaa tccccgcttt tgcgaaagag tgggagctgg
     2881 caaccctcag cccgaagggc gtcacaatcg tagcccagca c
//
LOCUS       DASRKK010000535         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1218412, whole genome shotgun sequence.
ACCESSION   DASRKK010000535 DASRKK010000000
VERSION     DASRKK010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218412"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /gene="nhaA"
                     /locus_tag="VFC15_12195"
     CDS             <1..1069
                     /gene="nhaA"
                     /locus_tag="VFC15_12195"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZM10958.1"
                     /translation="VHGELADMRRAALPIFGALGGMIVPALLYMALNVGHAGQHGWGV
                     PMATDIAFSLGVLGMVKGVPQDLKIFLLTLAIADDIGAIAVIAIFYSDSLNLESLVIG
                     LLLLAVIFLLRKVGISQPVLYFVLGVGFWAAILRSGIHATIAGVILGFMVPTTATLSL
                     REFQEIGGGIVEQFREALSRGDEKTAKNLLGSIEQLVHGTESPSELLTRKLNDWVSFL
                     VLPLFALANAGVTFSGEAWRELLTSRIAWGILLGLVVGKPLGIIGFARLAVRSGFAHV
                     PNGVTWRQLSAVGILAGIGFTVSIFISSLAFGDPSHLLEAKTGVLAASLLAGVLGYLA
                     LRDGGRLNQSSNKGDPHLNLD"
     gene            1487..2314
                     /locus_tag="VFC15_12200"
     CDS             1487..2314
                     /locus_tag="VFC15_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10959.1"
                     /translation="MRSRLLTLTFIAILAMSFAVCSKKDQSQNTGAPASDTSAATQAN
                     PPATTSQPVGSQPATSQMAPAQQPPQGQQMTPAPAQEMAQAPEPEPPPPLVVPAGTSI
                     VVRMGNSITSKTANPGDTFTGSLAHAVVVGGVVAIPARARVSGTVIDAKSPGKFKGEG
                     VLSIALTAVNVGGAPVAIQTSTYTKIVKGKGKRTAKMAGGGAGAGLLIGGLAGGGKGA
                     LIGGLVGAGAGTAGAAATGNKEVEIPAESAVTFRLGHSITVMRPAKKSSQDQMAPNQ"
BASE COUNT          523 a          739 c          844 g          602 t
ORIGIN      
        1 cgtacatggt gaactcgccg acatgcgtcg agcggcgctg cccattttcg gcgccctcgg
       61 cggcatgatt gttcctgcgc tcctctacat ggccctgaat gttggacatg ccgggcagca
      121 cggttggggc gttcccatgg cgactgacat tgcgttttcg ctgggggtgc tgggtatggt
      181 caaaggagtt ccccaagacc tgaagatctt tctcctgacc ctggcgattg ccgacgacat
      241 cggcgccatc gcggtgattg cgattttcta tagcgactcg ttgaacctgg agtccctggt
      301 cataggcctg cttctgcttg cggttatttt cctgttgcgc aaggtgggca tttcgcagcc
      361 ggtcttgtac ttcgtgctgg gcgtgggttt ctgggcggca attctgcgat cgggtattca
      421 cgccaccatc gcgggcgtga ttctgggatt catggttcct acgaccgcga ccttgtcgtt
      481 acgcgagttt caggagattg gcggtggaat cgtggagcaa ttccgagaag cactgtcccg
      541 cggcgacgag aaaacggcca aaaatttgct tggctccatc gagcagttgg tgcacggcac
      601 ggaatcgccg tccgagctct tgactcgtaa gttgaatgac tgggtttcat ttctggtact
      661 gcctttgttc gctcttgcca acgctggagt cacgttttcg ggagaagcgt ggcgtgaatt
      721 gctgacgagc cggattgctt ggggaattct gctcgggctg gtcgtgggaa agccgctggg
      781 aatcatagga tttgccaggc tggcggttcg ctcgggcttt gctcatgtcc ctaacggggt
      841 cacatggcgg cagttgagtg cggtcggaat tctcgccggc atcggcttta ccgtctctat
      901 ttttatcagc tcgctggcgt tcggcgaccc ctctcatctg ttggaagcga agacaggagt
      961 gctggcggct tcactgttgg caggggtact ggggtatctc gccctgcgcg atggcggacg
     1021 tctaaaccag tcctcgaata agggtgaccc tcaccttaac ctggattagt catctgtttc
     1081 ggcagctgaa gacagcaaac tttgaagggg cacggcttca gccgtgccgt tacgtgtgca
     1141 tgaagagttg ggcttcagcc cctgagggaa ctcgcacttc gtctcaacac cgaagtccct
     1201 cagtggctaa agccaccatg atgatagcgc tcacggcacg gctgaagcca tgccccttca
     1261 aagcgtgaga tccgcatgaa taatgcaggc tagtgagact taaattccgg gaaacacgat
     1321 acaatttgtg gggttcttaa ccgcgctagg ctggtctttg tagcgcattc gtctcgaacc
     1381 gtagcgcctt gcgggccttc attccggcag cacaggagca atgatttcac gcacggtagg
     1441 cggctggatg tagtctcgtc cggcgtcaca gagaaaggat gatgttatgc gttcccgatt
     1501 actcacgctc acgttcatcg caattctcgc gatgagcttc gcggtctgca gcaagaaaga
     1561 tcaaagccag aacacaggag cgccggcgag cgacacttcc gcagcgacgc aggccaaccc
     1621 acccgcaacc accagccaac cggtcggcag tcagcctgcg actagccaga tggcgccagc
     1681 gcagcagccc ccccaagggc agcagatgac gccagcgcca gcgcaggaaa tggcacaagc
     1741 tccggagccg gagccgccgc ctccgctggt ggtcccggcc ggaaccagca tcgtggtccg
     1801 gatgggaaat agcatcacat cgaaaacagc gaatccgggc gacaccttta ctggttcgct
     1861 ggcgcatgcg gtcgtggtag gcggggtggt cgcgattcct gcccgtgccc gcgtgagcgg
     1921 aacggtcatc gatgccaagt cgccgggcaa attcaaaggt gagggtgtat tgtcgatcgc
     1981 cttgaccgcc gtcaatgtcg gcggagcgcc ggtggctatc cagacgtcta cttataccaa
     2041 gatcgtaaag ggtaagggca agcgcactgc gaagatggcc ggaggcggag caggcgctgg
     2101 tttattgatc ggtggtctgg ctggcggagg caagggcgca ctgatcggcg gtctggtggg
     2161 cgcgggcgcc gggacggcag gtgcggcggc caccggcaac aaggaggtag agattccggc
     2221 ggagtcggcg gtgacgttca ggctcggcca ttcaatcacg gtgatgcggc cggcgaagaa
     2281 gtcttcccag gatcaaatgg cgccgaacca gtaacccgtc aacatcggaa atgcagggag
     2341 gccttcgggt ctcccttttt tgatgcttgc agaagccagt ttatcttgta ccgggtatag
     2401 cagacctttc atctggcatg aacttgaagg tgtcatcctg agagtttgtg acttttttga
     2461 atttccgcgt ttttttggca tgtaagttgt ctgttttcag catcggattt tgtgaaaaac
     2521 caataaagtc acagcctctg agcggagcgc ccgcaaagaa tttccggccc gcttcctttg
     2581 cgggtcgggc gggcgcgcag tcgaaggatc tgcgtttgtt gccggcggcg cgacggcacc
     2641 ggcgaaccac agatccctcg actcgggccg cgaagcgcgc cctccctcgg gatgacaaca
     2701 taagcttt
//
LOCUS       DASRKK010000536         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_436590, whole genome shotgun sequence.
ACCESSION   DASRKK010000536 DASRKK010000000
VERSION     DASRKK010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436590"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            696..1298
                     /locus_tag="VFC15_12205"
     CDS             696..1298
                     /locus_tag="VFC15_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10960.1"
                     /translation="MRTKSRLTKVVFNVLLLTSLLLLVSIAGFARDKNETIDATAWGT
                     STQLGKNIGVTLIIYEWSTPEDKAVLTEAFQKGSNKGLVTALQKMKAVGHINITGTLG
                     YDVSFIRLIPTPTGRKIRFVTNRQIRFGEAFYDTQSQSFDLTAGEIDINDQDKSKSAG
                     VLYPAAQLGIGDDGQLTLNLNQNAWRLGDIIDWKGTTGEN"
     gene            1406..1915
                     /locus_tag="VFC15_12210"
     CDS             1406..1915
                     /locus_tag="VFC15_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10961.1"
                     /translation="MKIQRCFTIHCALLLLPVLLIASFATAEDKKKDNAKDACANPNP
                     ASLCSADNTCGSASAPCTVNVKRTSYSSSATPSIPGAKGNDLFCVKAGTTVTWQSTSK
                     GTGFLIDPGAKSPFDPPGTIMGGSEKSVSVVAKTPGCFTYHLSATAQNGTYGKSKAAQ
                     AQLIVIGGQ"
     gene            1954..2724
                     /locus_tag="VFC15_12215"
     CDS             1954..2724
                     /locus_tag="VFC15_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HZM10962.1"
                     /translation="MDSVVVEVSVVLAAMLLSAAGVVVVRRHADVEWLEHHHEIAGYF
                     VTIIGTLYAVLLAFAVFAVWNDFKQASSNLEEEANRIGDLSRMAKGLSEPLRENVRTA
                     LAAYVRSVLEDEFPAMAEGRDSPRTWQAAGNLWNVYRSGAPGDDKSRFYYEESVKRLV
                     EMGDYRRLRLLASHGTVPGILWWLLCTGAVLLVGFTYFFALPSIRSQVVMTAALGGFL
                     AFTLLLIAALNGPFDGTVRVPADPVKIELSHLSAAQSE"
BASE COUNT          666 a          860 c          897 g          708 t
ORIGIN      
        1 gtcatggtgg cgccgctgcg tccgtcgatc actccctgga cttgggagct ctgcgcggcg
       61 gcgacgccgc aggtcagcac tacggctgca actgtcactg caaccacgaa ggcgattctt
      121 gcaatcctgc ttctgttcat aatcttcctc tcgttaagta cgggtatgtc cggcgcgagc
      181 gatctatccg agtcgccgag gttgaactaa cgaaccagca ccctgcgcca tatgcgttac
      241 acgggacggg atccgggagg atgatggtcc gctcaatttg gtttatctgg aacgacagca
      301 tagatagtat tacttaatgg gcttctggac actggtaaaa ttagtagagg tgacaaaaat
      361 cttttgcaga tgagtcgcac ggaactcgcg gtctggcaat gccgattgag taccggaacc
      421 tggcaagagc gaagttgacg caatccgacc cggccaatct atactggcgg caggcctgcc
      481 gtcctcgtgc tcgaagtcca ggcgccttga cgcgcgatcg cagcccggtg gatggcaatt
      541 ggcgctaggg aagcagcttt ccatgccggc actaaccgtt tttgattgac gtacaggtac
      601 ctttcatagt tatcttctgg ctagccgcca acgtaccatg atcggcaagt cggacaacgg
      661 tctctgccat aagtgcagcg acgagaagag ggaacatgcg cactaagtca cgccttacga
      721 aggtcgtttt caatgttcta cttttgacga gcctgctgct cttggtgagc attgcgggtt
      781 ttgcacgaga taagaatgaa accatcgatg ccactgcctg gggcactagc acccaactgg
      841 gaaagaacat tggcgtcacg ctgatcatct acgagtggtc gacgccggag gataaagccg
      901 ttcttaccga ggctttccag aaaggttcaa acaagggatt ggtgaccgct ctgcaaaaga
      961 tgaaggcggt gggccacatc aacatcacgg gtaccctcgg gtacgacgtc agctttatca
     1021 ggttgatccc aactccgacg gggcggaaga tccgcttcgt aaccaaccgc cagatcaggt
     1081 ttggcgaagc tttttatgac acccaatcgc aatcgtttga cttgactgcc ggcgagatcg
     1141 acatcaacga tcaggacaag agcaagagcg cgggtgtgtt gtatcccgca gcccagttgg
     1201 ggatcggcga tgatgggcag ttgaccctca atctcaatca gaacgcctgg agactgggtg
     1261 acatcatcga ttggaaggga acgaccggcg aaaactagcg cacggcttgg acggctttgc
     1321 ttgcggtccg aagtggctca gtggaacttt ctgaaaagcg agttcgtggc cgttgtgttt
     1381 gaatttcatt tcaaatgagg tgcttatgaa gatacagcgc tgttttacga ttcattgtgc
     1441 cctgctgctg ttgcccgtcc tgctgatcgc cagctttgcc acagccgagg acaagaagaa
     1501 agacaacgcc aaagatgctt gcgcaaaccc caacccggcg agtctgtgca gcgccgacaa
     1561 cacctgtgga tccgcttccg ctccctgtac cgttaacgtg aaacgtacct cgtactcatc
     1621 ctctgccacg cccagcattc cgggggcaaa gggtaacgat cttttctgtg ttaaggctgg
     1681 aactactgtg acgtggcaat ccacgtccaa ggggactggg ttcctcatcg atcctggtgc
     1741 taagtccccc ttcgacccgc cgggcaccat catgggcggc agcgaaaagt cggtttcggt
     1801 ggtggcaaag acgccagggt gctttacata tcaccttagc gccaccgctc aaaacgggac
     1861 ctacggcaag agcaaggccg cacaggcaca gctgattgtg atcggcggtc aatagggctc
     1921 tgccagccga cacgtcgaat ggagaacagg gctgtggatt ccgtcgtggt ggaagtctct
     1981 gtggttttgg cggcgatgtt actctcggcg gccggggtgg tggtcgtccg gcggcacgcg
     2041 gatgtcgagt ggttggagca tcatcatgag atcgccggct attttgttac catcatcgga
     2101 acgctttacg ctgtcctgct tgccttcgcc gtcttcgcag tgtggaacga tttcaagcag
     2161 gcgagttcca atctcgaaga ggaagccaac cggattggcg atctctcgcg catggcaaag
     2221 gggctttccg aaccgttgcg cgaaaatgta cggactgcac tggcggccta tgttcgctcg
     2281 gtcttggagg acgaatttcc cgccatggca gagggtcgcg atagtccgcg tacttggcag
     2341 gccgccggaa atctatggaa cgtgtatcgc tcaggtgctc ctggcgacga caaatcgcgt
     2401 ttctattacg aagagtcggt gaagcggctg gttgagatgg gcgactatcg cagactgcga
     2461 ctccttgcca gtcacggcac cgtgcctgga attctttggt ggcttttgtg tacgggagcg
     2521 gtcctgctgg ttggcttcac ctactttttt gccttgccta gcattcgctc gcaggtcgtg
     2581 atgacagccg cactgggtgg ctttctcgca ttcaccctgc tgctcattgc tgcgttgaac
     2641 ggccccttcg acggaactgt gcgagtcccc gccgatccgg tgaaaatcga gctgtcgcat
     2701 ctatccgcag ctcaatcgga gtagaactga ccttaaacgc ccaggtgggc aggctctctt
     2761 ggtagcgcgg accttcagag cctgccctgg gcgcagtcga agggtccgcg cacaaccttt
     2821 attattcttg tcctccctcg ccagcttccg tccgcgaggg gtcgagtgag tacggcccgc
     2881 gtggcagtcg ttttgcttga cttccgatgc cgaaacgcag actctcgata tgctccgctt
     2941 aaaagcataa tccgcagcag aaacgttcgt tcacttgaac gactgaccaa accgcgaggg
     3001 tcgtcaacga cccgcccggg tcaatcgaat tattggagaa gaaccatgca cctgcgccgt
     3061 cgagcttccc ttctcagcat cgtcctcata ctttgttcca tatccggcat cgggcaagag
     3121 aagccggcca a
//
LOCUS       DASRKK010000537        21027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1218630, whole genome shotgun sequence.
ACCESSION   DASRKK010000537 DASRKK010000000
VERSION     DASRKK010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218630"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFC15_12220"
     CDS             <1..416
                     /locus_tag="VFC15_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM10963.1"
                     /translation="EVHPMIEAKTIWTDKDRFVGEANSGHALVIDTGAVKTASTPIEL
                     VLIGLCGCTASDVVGILRKKREPFVGLEVVAQAERATEHPKVYTQIKLIYRVRGKVSH
                     KAMEDAVRLSKEKYCSVSAMLEKTAKIEFEIEYLE"
     gene            687..1835
                     /locus_tag="VFC15_12225"
     CDS             687..1835
                     /locus_tag="VFC15_12225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM10964.1"
                     /translation="MSRLGTETAFEVLVRARALERQGKNIVHLEIGEPDFDTPGNIVE
                     AAVGALHMGYTHYGPSAGLPDLREAIAEDVSKSRGVKFTSDEVVVVPGGKPIIFFTML
                     ALVEEGDEVIYPNPGFPIYESMINFLGAKAVPIALREERDFRLDVNELKSLINDRTKL
                     IILNSPQNPTGGVLTKGDIYDIADAIGDRDIMVLSDEIYGRLLFEGEQFSISAVEGMK
                     DRTIILDGFSKTYAMTGWRMGYGVMRADLATHIARLMTNSNSCTASFTQVAGIEAIRG
                     DQSETEHMRLEFQKRRNVFVNGINQIKGFSCRLPKGAFYTFPNITQTGWASKKLADAL
                     LDEAGVAGLSGTAFGVFGEGYLRFSVANSVENLQDALDRIDQWTKKNL"
     gene            2013..3149
                     /locus_tag="VFC15_12230"
     CDS             2013..3149
                     /locus_tag="VFC15_12230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZM10965.1"
                     /translation="MTEIDTPRHRIFATADIGDAAFARLRERGYEVEVYPHPEPPPKS
                     VILEKVHGGIDGLITTLRDMIDVEVFEAGKGTLKCVAQYAVGVDNIDRAAANRYKIPF
                     TNTPDVLTEATAEFAVFALAALTRRLWSAEKLVRENQWGFWHPYLPFLGDEMTGKTIA
                     IIGTGRIGQAMAKKCSGFDMNILCYHPQRPDDQFISDLQSVMDLRYQTGLQRERTTVR
                     YASLEDALSRADFVSIHVPLVREGEGSNPTHHLFNETTLRRMKPTAYLVNTARGAVID
                     ESALVRALKEKWIAGAALDVYETEPLPPDSPLRDPEIEDRCRLYPHFASAGRVTRLSV
                     DPNRGMAGRCVQGLIDVLEGNYGGDIQKMPFVVNKEAFRGERSG"
     gene            complement(3222..3917)
                     /gene="purQ"
                     /locus_tag="VFC15_12235"
     CDS             complement(3222..3917)
                     /gene="purQ"
                     /locus_tag="VFC15_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZM10966.1"
                     /translation="MKFGVIIFPGSNCDHDAFWTISEVSKQPVTFLWHDSPDLQGCDA
                     IIVPGGFAYGDYLRTGAIAKFSPVMESVRKFAADGGLVLGICNGFQILCECGLLPGAL
                     MRNAGLKYICKPVHIRVENTNTPYTQNLTKGEVLQIPIGHMEGNYFCDDQTLAELNDQ
                     DRIIFRYVTPSGDVTDDANPNGSIQNIAGICNAGRNVLGMMPHPERASEPELGMTDGV
                     KIFNSLVSALVTK"
     gene            4001..4579
                     /locus_tag="VFC15_12240"
     CDS             4001..4579
                     /locus_tag="VFC15_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM10967.1"
                     /translation="MQFSRHIEITGGALWKWLVAQFLDSICVGLLWWAGLSYLHVTWG
                     PLWAVLAGLFQFIPHFGPALTLVGPAVAALIDGGLETFLWVLGLYGAIIMVDGLILQP
                     VIMRRTAKVPIWASLIVPIMLGMLIGFWGFVLAAPLLAIIFAYRAVAKRTARKGGGAD
                     APHQTPPGRDITGGRLISPGGVDSESERTEVL"
     gene            4677..8150
                     /gene="dnaE"
                     /locus_tag="VFC15_12245"
     CDS             4677..8150
                     /gene="dnaE"
                     /locus_tag="VFC15_12245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZM10968.1"
                     /translation="MSEQFVHLHLHTDFSMLDGACEIGKLVDRVKELDMPAVAMTDHG
                     NIFGAVEFANAAKAAGVKPILGCELYVCKKEDHRAAPEGDSYNHLIVLAENDEGYRNL
                     VKVVSEASLHGFYYKPRVSKKFLSEHSKGLIALSGCLKGEVAEELTFEHYDKAREAAS
                     TYQDIFGKGNFFLEIQNQGLPEEHRIQNNLLKLEQELGIPMVATNDSHYLCEDDAHAQ
                     DVMVCIQTGKSINDTNRLKFANNQFFVKSYPEMQKEFLGYEQFLHRTLEIAERCNTKL
                     EKVHDPFPQFEVPAGYSIPDYFEHVTREGFAGRLNLLRELQAQGRLKHSLEDYEQRLA
                     REIGIIRQMQFPGYFLIVWDFIRYAREHNIPVGPGRGSAAGALVAYALRITDIDPLQN
                     ELLFERFLNPERISLPDIDIDFCMNRRGEVINYVTQKYGRDQVAQIITFGTMACKAAI
                     KDSGRAMDIPYSEVERIVKMVPPTLNITIDQALKDSPSMATAYETEPQVKELIDTAKR
                     LEGLVRNSGVHAAGVVISPQPLTDLVPLHRTKNDEIVTAYDMKAIEKLGLLKMDFLGL
                     TTLTIIDDALRLIEQVRGERLDLQTLALDDAETYEKVFHTGLTSGVFQFESQGMRDVL
                     RRYKPTAVEDLTALNALYRPGPIQGGMIDDFIDRKWGRKKIEYELPELEMMLKETLGV
                     IVYQEQVMQIANRLAGYSLGEADLLRRAMGKKDADEMAKQRQRFVKGALDRGFPQKKV
                     EKIFDLMEQFAGYGFNKSHSAAYALLAYHTAYLKTHYAVEFMAALLTSVSGSTDDVVK
                     YINECREMGIAVEPPDVNVSDAYFTPHGEAIRFGLAAVKNVGHNAIESIVVARKEIGA
                     FKSIFQFCEKVDLRLLNKRVIESLVKSGAMDAMGRRSQLMAVIDIAMEHAQKSQHDAA
                     MGQHGLFGVFAEDKTAQTEKPLPNIPDWDEHQRLSAEKEILGFFITGHPLEKYRDKLL
                     DFSALTTEELGQLKSSTGRDEVLIGGILKAVRVSKSKKGDLYAQGRLEDMNGSVDILC
                     FSESFKRLTEKLKLDVPVLVKGSVRLEEGSNAKLMISDITPLEQAEPKLARSLRIKIP
                     LETASPGTVDALHTLCAERKGAAKVLFDLERKGDFTVVMEVEGYNVLPDRSFINRVEE
                     LCGRGSVRIID"
     gene            8178..9134
                     /locus_tag="VFC15_12250"
     CDS             8178..9134
                     /locus_tag="VFC15_12250"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZM10969.1"
                     /translation="MEVPSNAHSELQHVEEQIEQLQSAAGDNQEALKQLEGLQERVEA
                     LRRQISGKLTPWERTELARHPMRPYPLDYVERIFPDFSEIHGDRNFGDDPAMVCGMAR
                     FHGREVAVLGTQKGRDTKQRVYRNFGQPNPEGYRKALRFMKFAEKWERPVIALIDIVG
                     AYPGLGAEERGQAEAIAHNLREMARLRVPFIAAITGEGGSGGALAIAVADCVLMQENS
                     IYSVISPEGCASIMWRDSAKKDIAAAALRITAKDNFEMGFCDEVIPEPPGGAHHDYDT
                     AAVALAEALDRHLGELEKMSTQDMLDARYKKFRNMAQYYETV"
     gene            9312..11150
                     /locus_tag="VFC15_12255"
     CDS             9312..11150
                     /locus_tag="VFC15_12255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZM10970.1"
                     /translation="MATTEIAVHGSSNAADRKPIVNDFSIQVATANGSGSQSANSILL
                     RSIFQMGVPVSGKNLFPSNIAGLPTWFTIRASKKGYVARKKEIDFVVAMNPETAREDV
                     LALAPGAAVLYDEPLKLSELRSDVVFYSAPFDKIVASVCPEAKLRKLVKNMIYVGVMA
                     QLLGLDMAEVEKALRKQFAKKVKAADLNWNAAKAGYDYAVASLTKADPFTIERMDKTA
                     GMIIVDGNSAAAMGCMFAGVTVVTWYPITPSSSLVETLIDYMKAYRVGADGKSTFAIV
                     QAEDELAAIGMVLGAGWAGARAMTATAGPGISLMAEFAGLGYYVEIPGVIWDVQRVGP
                     STGLPTRTSQGDIEFVAKLSHGDTFHPMLLPCSVAECFSMASEAFNLAEHLQTPVFVM
                     SDLDLGMNNWMSEPFEYPKEPINRGKVLSSEDLVRLGGFSRYKDVDGDGVGYRTLPGN
                     ENPQAAWFARGSGHNEKSQYSERPDDYQNNLDRLRHKFENARTLVPKPELVRNDGNDV
                     GIIAYGTSHHALIETLDQLQSENGINVDYLRLRAFPFTSEVQKFIDAHKRIYVVDQNR
                     DGQMFQLLKLDVPAEEVVRLRSVRHYNGLPLDARSVTDEIVSQEGK"
     gene            11151..12191
                     /locus_tag="VFC15_12260"
     CDS             11151..12191
                     /locus_tag="VFC15_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZM10971.1"
                     /translation="MATTAAPQPKTNRVGLTVLDYRGGKTTLCAGCGHNAISERIIDA
                     FYDMGITPERVVKMSGIGCSSKSPAYFMSRSHSFNSVHGRMPSVTTGAVLANSKLIAL
                     GVSGDGDTASIGIGQFVHLMRRNLPLIYIIEDNGVYGLTKGQFSATADLGSKLKTGVI
                     NDLPPIDTCALAISLGATFVGRSFSGDKRQLHTMLKAALAHRGTVMIDVVSPCVTFND
                     HEGSTKSYKFVKDHEEPLHDLNFVPAFNEIDVEYDEGSSVNVTMHDGSSLRLRKLEED
                     YDPTNKIGAVKRLMESHEADEVLTGVFYLNTQAPNFIDMLNMTDEPLATLPESVTRPS
                     RKVLEECMEELR"
     gene            complement(12252..12797)
                     /locus_tag="VFC15_12265"
     CDS             complement(12252..12797)
                     /locus_tag="VFC15_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM10972.1"
                     /translation="MRHWRVGKDHKVGIVGLGGLGHMGLKLAHAMGAHVVLFTTSAHK
                     VEDAKRLGAHEIVHTKDASQLEKHVNSFDLIMDTVSAEHDLNIYLNLLKRDATMVLVG
                     APEKPVPVAAFSLIPKRRQFAGSMIGGLPETQEMLDFCATHNIVSDIEIIPIQKINEA
                     YERMLKSDVKYRFVIDMASLK"
     gene            complement(12801..13346)
                     /locus_tag="VFC15_12270"
     CDS             complement(12801..13346)
                     /locus_tag="VFC15_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZM10973.1"
                     /translation="MPSTDLEVAPPKQATKTVTTAGYGAHSATTPLAPFSFERREPGP
                     EDVQIDILYCGVCHSDLHTARNEWTNTNYPVVPGHEIVGRVSKVGSQVKKFKERDLAG
                     VGCMVSSCGHCASCKEGLENYCENVPEFTYNSEDKHLGGVTYGGYSKSIVVDESFVLR
                     IPTNLNLAAVAPLLCAGITTY"
     gene            13567..13875
                     /locus_tag="VFC15_12275"
     CDS             13567..13875
                     /locus_tag="VFC15_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10974.1"
                     /translation="MAKPAVQFEVACPCCGALLKIDVDAKAVIAHTPKAVPKTFEDIE
                     HAARAMKERDSRKESIFQQSVEAQKNSADLLEKKFQEAVRKAKETPDTGKPLRDFDLD
                     "
     gene            13885..14577
                     /locus_tag="VFC15_12280"
     CDS             13885..14577
                     /locus_tag="VFC15_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZM10975.1"
                     /translation="MKPKPLLLGHRGCRDGSLGLHKSALPVENSLAAFEYAISHGCDG
                     FEFDVRQTHDGRNVLCHDPYCNRLPIAATNYAELVDPDGNLLPTLDYVLQQFGDRAFL
                     DIELKVRGAEKAVVAALHAVPPQRGFIVSSFLPDVLMRVCNIDSSLPLGYICERDYAL
                     GIWRGLPIQIVLPRFDLVEKELIEEVHRHGKKLVTWTVNSARRMEELDAWGVDGLISD
                     DPALLCQTFHRE"
     gene            14612..15517
                     /gene="mazG"
                     /locus_tag="VFC15_12285"
     CDS             14612..15517
                     /gene="mazG"
                     /locus_tag="VFC15_12285"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZM10976.1"
                     /translation="MSTTGERFERAVSIMARLRGPGGCPWDREQTFDTIKPYTLEEAY
                     EVIEAIDARDWPELTGELGDLLLQVLFYSQMAAEEQLFSIDDVLDRLSNKLVDRHPHV
                     FGEVKAETSGEVLRNWQALKAEEKKKRQQASGDDVDGTKAGHPDSVLHGISSAMPSLM
                     AAYKISSRAAQVGFDWPNIEGLFDKLEEETDELRENLEEYPEPGPRPVGASSTASARG
                     VKVSEELQARLEDEVGDLLFVLVNVARYLALDPESALRKTNCKFRRRFEYLEDRLREQ
                     GRKPSDASLDEMESLWQESKQQEKP"
     gene            15484..16287
                     /locus_tag="VFC15_12290"
     CDS             15484..16287
                     /locus_tag="VFC15_12290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM10977.1"
                     /translation="MAGVEAAGKAVTVETLRLQNCCELEEFRACVALQQEVWGFSDAE
                     LAPLRIFSLAPKIGGQVVGAWEGNSLVGFAMAIPGSRNGRPYLHSHMVAVKEGHRNTG
                     LGRKIKLFQREDAIAHGYELMEWTFDPLEIKNAYFNLERLGAIARRYNVNQYGITNSP
                     LQGFLPTDRLVAEWWMTSRRVQTVLSSGHHSPIEEEERIEVPGEIYAWKAEPAMRSRA
                     EQIQSRNREHFQSAFSRGLACLGYERDADGNGCFQLGRWDEDWSYAANS"
     gene            16303..17421
                     /gene="menC"
                     /locus_tag="VFC15_12295"
     CDS             16303..17421
                     /gene="menC"
                     /locus_tag="VFC15_12295"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZM10978.1"
                     /translation="MKIEAITLLELQIPLVHFFETSFGRTYTRRVLLLTLHCDGLEGW
                     GECTAGEDPYYSEEYIEGAWDVIVRYLGPSLIGRTLSAGREVPALLAKVRENRMAKAA
                     LENAAWDAEAQEKGIPLWRLLNGTQREVACGVSIGIQDSHDQLMQKIETEHAAGYQRI
                     KLKVKPGWDVDVLAKVRARWPDILLSCDANSAYTLDDMEHLKTFDRFKLLMIEQPLWS
                     DDFYFHARLQKQLHTAICLDEAIRSSRDAEAALELGACRIVNVKVGRVGGLTDAIAVH
                     DVAQRFGVPVWCGGMLESGVGRSHNVALSTLPNFKLPGDVSASKRYWKEDIIEPEVEV
                     SPQGTIAITDKPGRGYELRRDLIERLTVRKETLRAASF"
     gene            17422..18087
                     /locus_tag="VFC15_12300"
     CDS             17422..18087
                     /locus_tag="VFC15_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM10979.1"
                     /translation="MQRKVIAELLDDDLGTSDEIAKSLVDLRHINNWFGGTRTTTALL
                     RRIASATGETKLSLLEIGAGRGDLPVQACSRLAAANIELRVTLVDRIPSHLPRNTVNS
                     VAADALHLPFRDESFDVVSCSLFVHHFEPDELPLLVNEALRVCSKAVLINDLIRSRVH
                     LGLVYMGLPLFASPITWHDAPASVKRAYTLDEMESMLAKLPGKRLEITRHYLYRMGVL
                     LWK"
     gene            18084..19181
                     /locus_tag="VFC15_12305"
     CDS             18084..19181
                     /locus_tag="VFC15_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM10980.1"
                     /translation="MKRCDFDIAVIGGGPAGASAAITAARFGASVALLEAGEFPRHKV
                     CGEFVSAESLDVLRDLLRSQSIAAPVLCDAPVINRVRLLLGGRVITARVVPPALSIAR
                     YDLDFLLWQAAQEAGVHTQSTCEVRMIQGDGPFELDTCSGAIHAASVIVAAGRWSRFA
                     PRSPLPGGPKWIGLKAHFREDRPPRSTDLYFFDHGYCGVQLVDDTTVNACAMVRSDRA
                     TSLDEVFALHPALAERSRNWRAMTEPVTTAPLIYRAPQPVKDNMLFAGDAAAFIDPFV
                     GDGISIALRSGRMAAMSLRAFLNRGSSLDLALTDYENSYERQFVPLIAAASRIRGVLS
                     WSASVRMIAFEFLRVPGLMPYVIRKTRRAAS"
     gene            complement(19190..19780)
                     /locus_tag="VFC15_12310"
     CDS             complement(19190..19780)
                     /locus_tag="VFC15_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10981.1"
                     /translation="MSGERVQRRSTGFTEFMKWLAREEGLSVLDLGPTSPANITFFIG
                     LGHRFHQEDVLRLSSDKSLIVSNSEGQKTIDVERFLRENLCFERQEFDAVMFWDLADY
                     LPEALVKPVIERIHVAMKPSAILFSFFHTKDAGPEAPFYRYNIAARDTIEMQPASPYK
                     LQRIFNNRHVENLFHDYNSIKFFLGRDNIREVLVIR"
     gene            19833..20003
                     /locus_tag="VFC15_12315"
     CDS             19833..20003
                     /locus_tag="VFC15_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10982.1"
                     /translation="MLGDTGHYCKGSATQAPISRELPKDLLQAGAAAVPTKGLIESKK
                     KRDAGARPDKIV"
     gene            complement(20010..20591)
                     /locus_tag="VFC15_12320"
     CDS             complement(20010..20591)
                     /locus_tag="VFC15_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZM10983.1"
                     /translation="MWKSTRKEDEFLTPAPEQPAPKPAYTPPPPAVRAAEPVRSDVPR
                     SVDVATIGKSVIVKGELSGSEDLYVDGEVEGSISLRGQSLTIGPNGRVRANIEARNVI
                     VHGRVDGNIQASDRVELRKSASLSGDISTARVAIEDGAFFKGSIDIQKAEPAPRLEPK
                     PAAVVAAAPTPVPAPIPVAAPATQGSLLEPKKF"
BASE COUNT         4493 a         5684 c         6515 g         4335 t
ORIGIN      
        1 aggaagtgca tcccatgatt gaagccaaga cgatctggac cgacaaagac cgctttgtgg
       61 gcgaggccaa cagcggccat gcgttggtta tcgataccgg cgcggtgaag acggcaagca
      121 cgccgattga gctggtgctg atcgggctat gcggatgcac cgcaagcgac gtggtgggaa
      181 ttctacgcaa gaagcgcgag ccttttgtcg gcttggaagt ggtggcgcaa gcggagcggg
      241 cgacggagca cccaaaggtt tatacgcaaa tcaagttgat ttatcgggtg cgcggcaagg
      301 tttcgcacaa ggccatggaa gacgcggtgc gcctgtcgaa ggagaagtac tgttcggtgt
      361 cggccatgct ggagaagacg gccaagatcg agttcgagat tgaatacctc gagtagcggg
      421 tggcatcgcg gtctcgtcgc ctttgcttgc atccaatgat taccaggctg gtaccgttgc
      481 acgggcgtgg gcgaggctca cgtcggtttg tagtgcgccg cgtcacaatg atctgggctg
      541 cgcgtcactg cgctgggttc aggccggtac caaaatgtga gatggcttgg ggccgcctat
      601 gtcccctggg ccgcggtaga tcgaggaaaa tcagcatgag ctcacttata acttccgcac
      661 cagcaaaact taatctggcg aaaagaatgt ctcgactcgg aaccgagacg gcctttgagg
      721 tcctggtccg cgcccgcgct ttagagcgcc agggcaagaa catcgtccat ctcgaaatcg
      781 gcgagcccga tttcgacact cccggaaaca tcgtcgaagc cgccgtcggc gcgctgcaca
      841 tgggctacac gcactatgga ccctcggcgg gtctgcctga tttgcgtgaa gccatcgccg
      901 aagatgtatc gaagtcgcgc ggcgtaaagt ttacctccga cgaggttgtg gtcgttccgg
      961 gaggcaagcc gatcatcttc tttaccatgc tggcgctggt ggaagagggt gacgaggtca
     1021 tctatcccaa tccgggcttc ccgatttacg agtcgatgat caacttcctg ggcgcgaagg
     1081 ctgtgccgat tgcgctgcgc gaggaacgcg actttcgcct tgacgtcaac gaactgaaga
     1141 gcctgatcaa cgatcgcacc aaactcatca ttctcaactc gccacagaat ccgaccggtg
     1201 gcgtgctgac gaagggcgac atttacgaca ttgctgacgc cattggcgat cgcgacatta
     1261 tggtgctgag cgacgagatt tacggtcgct tgctgtttga aggcgagcag ttcagcatct
     1321 cggcagtcga gggcatgaag gacagaacga tcatcctcga cggcttctcc aagacctatg
     1381 ccatgacggg atggcgcatg ggctatggcg tgatgcgggc cgacctggcg acgcacatcg
     1441 cgcgcttgat gacgaactcg aactcctgca ccgcgagctt cacgcaggtg gcggggattg
     1501 aagcgattcg cggcgatcag agcgagaccg agcacatgcg gctggaattc cagaagcggc
     1561 gcaacgtgtt cgtcaacggc atcaatcaga tcaagggatt ctcgtgccgc ttgccgaaag
     1621 gcgcgttcta caccttcccc aacatcacgc agacgggatg ggcgtccaag aagctggccg
     1681 acgcgctgct cgacgaagcc ggagttgctg gactttcggg tactgcattc ggagtgttcg
     1741 gagaaggcta tttgcggttc agtgtggcga attcggtgga gaacctgcag gatgcgctcg
     1801 accgcatcga ccagtggacg aagaagaacc tgtagcattt ctttcttttg aactcggccc
     1861 gctatcgagc gggccttttt gttgggctat gatgggcagc gcgggtccgg ccaaaatact
     1921 tcaccacgga ggcacggcgg acacggagag gaacttgaac aaaatacgca agttctccat
     1981 atgttctgta atggacggtg tccgaagttg ccatgactga gatagacaca ccacgtcacc
     2041 gtatctttgc tacggccgac atcggcgacg ctgccttcgc caggttgcgg gagcgcggtt
     2101 acgaggtcga ggtgtatccg caccctgagc cgccgccaaa aagcgtcatc ctcgaaaaag
     2161 ttcatggagg aattgacgga ctcatcacga cattgcgcga catgatcgat gtggaggtgt
     2221 tcgaggctgg caagggtacg ctgaagtgcg tggcgcagta tgccgtgggg gtggacaaca
     2281 tcgatcgggc tgccgccaat cgatacaaga ttccgttcac caacacgccc gatgtgctca
     2341 ccgaagccac ggcggagttc gcggtctttg cgctggcggc actgacgcgc cgcttgtggt
     2401 ccgcagagaa gctggttcgc gagaaccagt gggggttctg gcatccttat ctgccgtttc
     2461 tcggcgacga gatgacgggc aagacgattg ccatcatcgg caccggacgc atcggtcagg
     2521 ccatggcgaa aaagtgcagc ggcttcgata tgaacatcct gtgctaccac ccacagaggc
     2581 ccgacgatca attcatctca gatttgcagt cggtgatgga cttgcgttat cagactggac
     2641 tgcaacgcga acgcacgacg gtgcggtatg cgtcattgga agatgcgttg agcagggcgg
     2701 atttcgtgag catccatgtg ccgctggttc gtgaaggtga gggtagcaat ccgacccatc
     2761 atctgttcaa cgagacgacg ttgcgacgga tgaagccgac agcctacctg gtcaacacgg
     2821 cgcgcggagc tgtgattgac gaatcagcct tggtccgcgc gctgaaagaa aagtggattg
     2881 ccggagccgc tctcgatgtt tacgagaccg agcctctgcc gccggattct ccactgcgcg
     2941 atccggagat cgaggatcgt tgcaggctgt atccgcattt tgccagcgca ggacgtgtga
     3001 cgcgtttgtc ggtcgatccc aaccgcggta tggcagggcg ttgtgtgcag gggttgatcg
     3061 acgtactgga agggaattac ggtggcgaca tccagaagat gcccttcgtg gtgaataagg
     3121 aggcgttccg gggggaaaga agtgggtagt ggatagtggg cagtggatag aaaagagcag
     3181 gcccaatcaa gcggccaacc ctgttgccac cgagatttcg cctacttcgt taccaacgct
     3241 gatacgagcg aattgaagat cttgacgcca tcggtcatgc cgagctcggg ctcgctggcg
     3301 cgttcgggat gcggcatcat gcccaagacg tttcggcctg cgttgcagat gcccgcaata
     3361 ttctggatcg agccgttggg attggcgtcg tcggtcacgt caccactcgg agtgacgtag
     3421 cggaagatga tgcggtcctg gtcgttcagt tccgccagcg tctggtcgtc acagaagtaa
     3481 ttgccttcca tgtgaccgat gggaatttgc agaacttcac ccttggttaa attctgcgtg
     3541 tacggcgtat tggtgttctc cacacgaatg tggacgggtt tgcagatgta cttcagtccg
     3601 gcgtttcgca tcagggcgcc gggaagcagc ccgcattcgc ataggatctg gaagccgttg
     3661 cagatcccga gcacgagacc gccatcggca gcaaacttgc ggacggattc catcactggc
     3721 gaaaacttgg cgattgcacc ggtgcgcagg tagtcaccgt aagcaaaacc gccaggaacg
     3781 atgatcgcgt cacatccctg caggtccggc gagtcgtgcc agagaaaagt cacgggctgt
     3841 ttggatactt ccgagatggt ccaaaatgcg tcgtgatcgc agttggaacc ggggaagata
     3901 atgacgccaa atttcattgc cgtgtgctcc aggaaggggt ttcggactca gtgtagcatg
     3961 ggggcagcga cctcctgatc gcgcgggcat ccaaagagtt atgcagtttt ccaggcacat
     4021 cgagatcacg ggcggcgcgc tgtggaagtg gctggttgcg cagtttcttg actccatctg
     4081 cgtcggattg ctgtggtggg ccgggctcag ctatctgcac gtgacgtggg ggccgctgtg
     4141 ggctgtgctg gccggattgt ttcagttcat tccgcacttt gggccggcgc tgacattagt
     4201 tgggccggca gtggctgccc tgatcgacgg aggactggaa acgtttctct gggtgcttgg
     4261 gctttacggc gcgatcatca tggtggatgg cctgattctg cagccagtca ttatgcgtcg
     4321 aactgcaaaa gtgccgatct gggcgtcgct gatcgtcccg atcatgctcg gcatgctgat
     4381 tggattctgg gggtttgtgt tggcggctcc cttgctggcg atcatttttg cctaccgcgc
     4441 ggtggccaag cgaactgcaa ggaagggggg gggagcggac gcgcctcatc agacgcctcc
     4501 gggtcgcgat attactggag gcaggttaat ttctcccggt ggagtggaca gtgagtctga
     4561 gaggactgag gtgctgtagg cgctcgcgca ggattgctgt cgcgattttg taggccctga
     4621 tttccacaac cgtcgcgaat acaatgagct atcacccgcg aggcctctga gtagcaatgt
     4681 ctgagcaatt tgttcatctt catctgcaca cagatttctc catgctcgac ggcgcttgcg
     4741 aaatcggcaa gctcgtcgat cgagtcaaag agctcgacat gccggccgtc gccatgacgg
     4801 accacggcaa tatctttgga gcggtggaat tcgccaatgc tgcgaaagct gccggagtga
     4861 agccgatcct gggctgcgag ttgtacgtct gtaagaaaga agaccaccgc gccgctcccg
     4921 agggtgacag ctacaaccac ctgatcgtgc tggccgaaaa tgacgagggc tatcgcaacc
     4981 tggtgaaggt cgtttcggag gcctcgttgc acggcttcta ctacaagccg cgggtcagca
     5041 agaaatttct gtcggagcac tcgaaggggc tgatcgcatt gtccggatgc ctcaagggcg
     5101 aagtagccga ggagttgacc tttgagcatt acgacaaagc gcgtgaagcg gcctcgacct
     5161 atcaggacat ttttggcaaa gggaattttt tcctcgagat tcagaatcag ggactcccgg
     5221 aagagcaccg catccagaat aacctgttga agctggaaca ggaactgggt attccgatgg
     5281 tggcgaccaa cgacagccac tatctgtgcg aggatgacgc gcacgcccag gacgtgatgg
     5341 tttgcatcca gaccggcaag tcgatcaacg acaccaaccg gttgaagttc gccaacaacc
     5401 agttcttcgt gaagagctac ccggagatgc agaaggaatt cctgggttat gagcagttcc
     5461 tgcatcgcac actggagatt gccgagcgct gcaataccaa gctggaaaag gtgcacgatc
     5521 catttcccca gttcgaagtt cccgcgggct attcgatccc ggactatttc gagcacgtca
     5581 cgcgcgaggg gtttgcgggc cgactgaacc tgctgcgcga actccaggcg cagggccgtt
     5641 tgaagcactc gctggaagac tacgaacagc gactggcgcg cgagatcggg atcattcggc
     5701 agatgcagtt ccccggatac ttcctgatcg tgtgggattt cattcgctac gcacgcgagc
     5761 acaacattcc ggtggggccg ggccgcggat cggccgcagg cgcgctggtg gcttacgcgc
     5821 tccgcatcac cgacattgat cccttgcaga acgagttgct gttcgagcgg ttcctgaatc
     5881 cggagcgcat cagcctgccc gacatcgaca tcgacttctg tatgaaccgt cgcggcgagg
     5941 tcatcaatta tgtgacgcag aaatacggcc gggatcaggt ggcgcagatc ataacgttcg
     6001 gcaccatggc ctgcaaagcc gccatcaaag actcaggccg ggcgatggac attccgtatt
     6061 cagaggtcga gcgcatcgtc aagatggtgc cgcctacttt gaacatcacc atcgaccagg
     6121 cgctcaaaga ttcgccgtcc atggcgacgg cctatgaaac cgagccacag gtaaaagaac
     6181 tgatcgacac cgcgaagagg ctggaaggcc tcgttcgtaa ttcaggtgtg catgccgcgg
     6241 gagtcgtgat ctcgccgcag ccgctgaccg acctggttcc actgcaccgc accaagaacg
     6301 acgaaatcgt gaccgcctac gacatgaagg cgatcgaaaa gctcggcctg ctgaagatgg
     6361 actttcttgg gctgaccacg ctcacgatta ttgacgacgc gttgcggctg atcgaacagg
     6421 tgcgagggga gcggctggac ctgcagacgc tggcgctcga cgatgccgaa acttacgaga
     6481 aggttttcca tacgggtcta acgtcgggag tgttccagtt tgaatctcag ggaatgcgcg
     6541 acgtgctgcg gcgctacaaa cccactgcgg tcgaggacct cacggcattg aacgcacttt
     6601 accgtcctgg accgattcag ggcggcatga ttgacgactt catcgaccgc aaatggggcc
     6661 gcaagaagat cgagtacgaa ctgccggaac tggaaatgat gctcaaggag acgcttggcg
     6721 tcatcgttta tcaggaacag gtcatgcaga tcgcgaaccg actggcgggc tactcgctgg
     6781 gtgaagcgga tttgctgcgc cgggcgatgg gcaagaagga cgccgacgag atggccaagc
     6841 aacggcagcg ctttgtaaag ggcgcgctcg atcggggatt cccgcagaag aaagtcgaga
     6901 agatcttcga cctgatggag cagttcgctg gttacggatt caacaagtcg cactcggccg
     6961 cctacgcgct tctcgcctac catacggcat acctgaaaac acattacgcg gtggagttca
     7021 tggccgcgct gctgacctcg gtatcgggga gcaccgacga cgtggtgaag tacatcaatg
     7081 agtgccgtga gatggggatc gcggttgaac ctccggatgt gaacgtcagc gatgcgtact
     7141 tcacaccgca tggcgaggcc attcggtttg ggctggcggc ggtcaagaac gtcggacaca
     7201 acgcgattga gtcaatcgtg gtggcgcgca aggagattgg cgcgtttaag tcgatcttcc
     7261 agttctgcga aaaagtggac ctgcgtttgc tgaataagcg ggtgattgaa tcgctggtca
     7321 agagcggagc catggatgcc atgggcagac gctcgcagct tatggctgtg atcgacatcg
     7381 ccatggagca cgcgcagaaa tcgcaacacg acgcggccat gggacagcac ggtctgtttg
     7441 gcgtgtttgc agaagacaag accgcgcaga ccgagaagcc attgcccaat attcccgact
     7501 gggatgaaca ccagcgactt tcggcggaga aagaaatcct ggggttcttc atcaccgggc
     7561 atccgctgga aaagtaccgc gacaagctgc tcgatttcag cgctctcacg accgaagaac
     7621 ttggacaact caagtcatcc accggccggg acgaagttct gattggcggc atcctgaagg
     7681 cagtgcgtgt gtctaagtcg aagaagggcg acctctacgc ccaggggcga ctggaagaca
     7741 tgaacggatc ggtggacatc ctatgttttt cggagtcgtt caagaggctc acggaaaagc
     7801 tgaagctgga tgttccggtg ctggtgaagg gaagcgttcg cctggaggaa ggatcgaatg
     7861 cgaagctgat gatcagcgac atcactccgc tggagcaggc cgagcccaag ctggcgcgca
     7921 gtttgcgaat caagattccg ctggaaactg cgtcacccgg tacagtcgac gcgctccaca
     7981 cgctttgcgc ggagcgcaag ggcgcggcca aggtgctgtt cgacctggag cgcaagggcg
     8041 acttcacggt ggtgatggag gtggaaggct ataatgtgtt gccggaccgc tccttcatca
     8101 accgcgtgga agaactgtgc gggcgaggca gcgtaaggat catcgattag tcccgacgag
     8161 tgagacacga ccaacctatg gaagtccctt ccaacgcgca ttcggaattg caacatgtcg
     8221 aggaacagat tgagcaactg cagagtgctg ccggcgataa ccaggaagca ctgaagcaac
     8281 tcgagggttt gcaggagcgc gtcgaggcac ttcgtcggca gatttcgggc aagttaacac
     8341 cttgggagcg caccgagttg gcgcggcatc cgatgcgacc gtacccgcta gactatgtcg
     8401 agcgcatctt tcccgacttc agcgaaattc acggagaccg caatttcggc gacgatcccg
     8461 ccatggtgtg tggcatggcg cgtttccatg gacgcgaagt ggccgtgctc ggtacgcaaa
     8521 agggccgtga caccaagcag cgggtctacc gcaacttcgg ccagccaaat ccggaaggtt
     8581 accgcaaggc gctgcgcttc atgaagttcg ccgagaagtg ggagcgtcca gtgatcgcgc
     8641 tgatcgacat cgttggtgcc tatcccggcc tgggagcgga ggagcgagga caggcggaag
     8701 cgatcgcgca caatttgcgc gagatggccc ggctgagagt cccattcatt gcagccatca
     8761 caggcgaagg cggaagcggt ggggcgctgg ccatcgctgt ggccgactgc gtcctgatgc
     8821 aggagaacag tatctactcg gtgatttcgc ccgagggatg tgcgtcgatt atgtggcgcg
     8881 actccgctaa gaaggacatt gccgcggcgg ccctgcgcat tacggcaaaa gacaacttcg
     8941 agatgggctt ttgtgatgag gtaatccccg agcctccggg aggcgcgcat cacgactacg
     9001 acaccgctgc cgtcgcattg gcggaggcgc tggaccggca tctcggtgaa ctggagaaga
     9061 tgtcaacgca agacatgctg gacgcccgct acaagaagtt ccgcaacatg gcgcagtatt
     9121 acgagacggt gtagcaggcg gggacgtgcc cttcggaggc ggccctcagc cggggcctcg
     9181 aggtctttag catcatttca gtacacccgg ttcatttctg tattagtttc cgccagcgaa
     9241 tggctgccct ataatgtatg caatcccctc gaccaacctt gtagcgtggg gaaccgagac
     9301 taggggtatt gatggcaaca actgaaattg cagtacacgg ctccagcaat gcggccgatc
     9361 gcaagcctat tgtcaacgac ttcagcatcc aggtagcaac cgcaaacggg tcgggatcgc
     9421 aaagcgccaa ctccatcctg ctgcgctcga tttttcagat gggtgttccc gtctccggca
     9481 agaacctgtt cccgtcgaac atcgccgggc ttcctacgtg gttcaccatt cgagccagca
     9541 agaaggggta tgtcgctcgc aagaaggaaa tcgatttcgt cgttgcgatg aatcctgaga
     9601 ccgcgcgtga ggacgttctg gcgctggctc cgggcgcagc tgttctctac gacgaacctc
     9661 tcaagctgag cgagttgcgc tccgacgtgg ttttctattc tgcgcccttc gacaagattg
     9721 tggccagcgt ctgccccgaa gccaaattgc gcaaactggt caagaacatg atctacgtcg
     9781 gggtgatggc gcaactgctt ggcctcgaca tggccgaagt ggagaaggct ctgcgaaagc
     9841 agtttgccaa gaaggtgaag gctgcggatc tcaactggaa cgcggccaag gctggttacg
     9901 actacgccgt agcatcgctg acgaaagctg atccgttcac catcgagcgc atggacaaga
     9961 ccgccgggat gatcatcgtt gatggcaact ctgccgccgc gatgggatgc atgtttgccg
    10021 gcgtcacggt cgtcacctgg tatccgatta cgccctcgtc atcgcttgtg gagaccctca
    10081 tcgattacat gaaggcttac cgggttggcg ctgacgggaa gtcgactttt gctatcgtcc
    10141 aagccgagga cgagctggcc gcgataggca tggttttggg ggctggctgg gcgggagcgc
    10201 gtgcaatgac cgccacggcc ggaccaggca tttcgctcat ggcggagttc gcggggctgg
    10261 gatattacgt ggagattccg ggagtgatct gggacgtgca gcgggttgga ccgtcgaccg
    10321 gacttcccac tcgcacctcg caaggtgaca tcgagtttgt tgccaagttg tcccacggag
    10381 acacgttcca tcccatgctg ctgccgtgtt cggtagcgga atgtttctcg atggcgagcg
    10441 aggcgttcaa cctggccgag cacctgcaga cgccggtgtt cgtgatgtcg gacctcgacc
    10501 ttggtatgaa caattggatg tcggagccgt tcgagtaccc gaaggagccg atcaatcgcg
    10561 gcaaggtgtt gagcagcgaa gatctcgtcc ggctcggtgg gttttcgcgc tataaggatg
    10621 ttgacggcga cggagtggga tatcgcacac tgcccgggaa cgaaaacccg caggcggcgt
    10681 ggttcgcacg cggcagtggg cacaacgaga aatcgcaata cagcgagcgt cccgacgatt
    10741 atcagaacaa tctggaccgg ctgcggcata agtttgagaa tgcgcggacc ttggttccca
    10801 agccggagtt ggtcaggaac gacggcaacg acgtcggcat catcgcctat ggcacttcgc
    10861 atcatgcgct gattgaaacg cttgaccagt tgcagagcga gaacggcatc aatgtcgatt
    10921 acctgcgtct gcgggcattt ccgttcacca gcgaggtaca aaagttcatc gacgcccata
    10981 agcggattta cgtggtggac cagaaccgtg atggccagat gttccagttg ctgaagctgg
    11041 atgtcccggc ggaagaagtt gttcgactgc gcagtgtgcg ccactacaac ggactaccgc
    11101 tcgatgcgcg atccgtgacc gatgagatcg tgtcccagga gggcaagtaa atggcgacca
    11161 cagccgcacc acaacccaag accaatcgcg tagggctcac cgtgctcgac taccgcgggg
    11221 gcaagaccac gctctgtgcc ggttgcggac acaacgccat ctcggagcgc atcatcgatg
    11281 ccttttacga catgggtatc acgccggaga gagtggtcaa gatgtcgggc atagggtgct
    11341 cgtcgaagag cccggcgtat ttcatgagcc gctcgcacag cttcaactcc gtgcacggtc
    11401 gcatgccgtc ggtgaccacc ggagcggtgc tggcgaactc aaagctgatt gcgttgggcg
    11461 ttagcggaga cggcgacacg gcttccatcg gcattggcca gttcgttcac ttgatgcgac
    11521 ggaatctgcc gctgatttac atcatcgaag acaatggcgt gtatggcttg acgaagggcc
    11581 agttctcggc cacggctgat cttggatcga agctgaagac tggcgtcatc aacgatctgc
    11641 cgccgatcga cacctgcgcg ctggctattt cgctgggcgc aacgttcgtg ggccggtcgt
    11701 tctcgggcga caaacgccaa ctgcacacca tgctgaaggc ggccctggcg caccgcggaa
    11761 cggtgatgat cgatgtggtc tcgccgtgcg tgacgttcaa cgatcacgag ggttcgacca
    11821 agtcctataa attcgtgaag gaccacgaag aaccgctgca cgacttgaat tttgtacctg
    11881 cgtttaacga gatcgatgtt gagtacgacg aaggatcaag cgtgaacgtg accatgcacg
    11941 acgggtcgtc gctgcggttg cggaagctgg aagaagatta cgatccgacc aataagattg
    12001 gggcggttaa acggctgatg gaatcccacg aggccgacga agtcctgact ggagttttct
    12061 acctgaacac gcaggcccca aatttcatcg acatgctgaa tatgacggat gagccgctgg
    12121 caacgttgcc ggagagcgtg actcgtccat cgcgaaaagt gctcgaggag tgcatggaag
    12181 agttgcgata ggcagactgc gccgcaaaac caaaagccct ccgcgaggag ggcttttaca
    12241 tgggcagagc gttacttcag cgacgccata tcgatcacga agcggtactt cacgtcgctc
    12301 ttcaacatgc gttcgtaggc ctcattgatc ttctggatag ggatgatctc gatgtcggac
    12361 acaatattgt gtgtcgcgca aaagtcgagc atctcctgag tttcggggag cccgccaatc
    12421 atggatccgg cgaactggcg gcgctttgga atcaggctga atgcggccac tggcacgggc
    12481 ttctcgggtg ctcccaccag caccatggtc gcgtcacgct tcaacaggtt gaggtagatg
    12541 ttgaggtcgt gttcggccga aaccgtgtcc attattaagt cgaagctgtt gacgtgcttt
    12601 tctaattgcg acgcgtcttt agtgtgaacg atttcgtggg cgcccaatcg cttggcatcc
    12661 tcgaccttgt gcgctgaagt ggtgaacagc accacatgcg ctcccatggc gtgggcgagt
    12721 ttcagtccca tgtgaccgag accgccgagg ccgacgattc cgaccttgtg atccttgccg
    12781 actctccagt ggcgcagcgg tcagtaggtg gtgatacccg cgcaaagtag cggtgcaaca
    12841 gcggcgaggt tgaggttggt cgggatccgc agcacgaacg actcatccac gacgatcgac
    12901 ttggagtaac cgccataggt cacgccgccg agatgtttgt cttcgctgtt gtaggtgaac
    12961 tcaggtacgt tctcgcagta gttctccagt ccctctttgc aactcgcgca gtggccacag
    13021 gagctgacca tgcagccgac gccggccagg tcccgttcct tgaatttctt gacttgcgat
    13081 cctactttgc tgacgcggcc gacgatctcg tgtccgggaa cgacgggata gttggtgttg
    13141 gtccattcgt tacgcgcggt gtgcagatcg gaatggcaga caccgcaata gagaatgtcg
    13201 atctgaacgt cctccgggcc gggctcgcga cgttcgaagg agaaaggcgc taacggagta
    13261 gtggcggaat gggctccata accagcggtc gtgacggtct tagtagcttg cttcggcggt
    13321 gcgacttcca aatcagtgct aggcatcggt caggtcctca cgataagtgg cccgcggttg
    13381 gtgatcacgg ggtaggtcaa gaagaaaaaa gtggtcaacg gattacgcga acttccatat
    13441 tgatgcatca aggcggcatt tgggcgcacc gtcagtccgc caatttgttc acttcgctat
    13501 ctcgcaggaa ggcgatgcaa ctaccactgt agccgttcgc ccgcgggctg cggtaagatt
    13561 gcgttcatgg ccaagcctgc ggttcagttt gaagttgcct gcccgtgttg cggggcgttg
    13621 ttgaagatcg acgtcgatgc caaggcggtg atcgcgcaca ccccgaaagc tgtcccgaag
    13681 acgtttgagg acattgagca cgcagcacgc gcgatgaaag agcgcgacag ccgcaaggaa
    13741 tcgatcttcc agcagtcggt ggaagcccag aagaattcgg ctgacctgct ggaaaagaag
    13801 tttcaggagg cggtccgcaa ggccaaggaa acgcctgaca ctggcaaacc gctgcgcgat
    13861 ttcgatctcg actgacgggc cgcggtgaaa cccaaaccac tgctacttgg tcatcgcgga
    13921 tgtcgcgatg gaagtcttgg gctccacaag tctgccttgc ctgttgaaaa ctcgctcgct
    13981 gctttcgaat atgcgatctc ccatggctgc gacggcttcg agttcgacgt gcgccaaacc
    14041 cacgacggcc gcaacgtgct gtgtcatgat ccttactgta accgcctccc catcgctgct
    14101 accaactacg ccgagctcgt ggatccagac ggcaacctac ttcccacact cgactatgtc
    14161 cttcagcaat tcggcgatcg cgccttcctg gacatcgaac taaaagtacg tggcgctgaa
    14221 aaggcggtcg tggccgctct gcatgccgta ccgccgcaac gcggtttcat cgtgtcgtcg
    14281 ttccttcctg acgtactgat gcgggtctgc aatatcgaca gcagcttgcc gctgggatac
    14341 atctgcgaac gcgactatgc actcggcatt tggagaggtc tccctataca aatcgtgctt
    14401 ccacgtttcg atctcgtcga gaaggagctg atcgaagagg tgcatcgcca cgggaaaaaa
    14461 cttgtgacct ggacagtgaa ttctgctcgc cgcatggagg agctcgacgc ctggggtgtc
    14521 gacggactga tctccgacga tcccgccctc ttgtgtcaaa catttcaccg cgaatagcga
    14581 aaaccacgct gcgtcattta cattggttgc gatgtcaacc acaggcgagc gattcgagcg
    14641 cgcggtgtcc atcatggcgc ggttgcgggg gcccggtggg tgtccatggg accgcgaaca
    14701 gaccttcgac acgatcaagc cgtacacact ggaagaagcc tacgaggtta tcgaggccat
    14761 cgatgcgcgc gattggccgg agctgaccgg agaactgggc gacttgctac tgcaggtgct
    14821 tttctattcg caaatggcgg ccgaggagca gctgttctct attgatgatg tgcttgatcg
    14881 gctctccaat aagctggttg accggcatcc acacgtgttt ggggaagtga aggcggagac
    14941 ttcgggcgaa gtactgcgca attggcaggc actcaaagcc gaagaaaaga agaagcggca
    15001 gcaggcttcc ggcgacgacg ttgatggtac caaggccggt catcccgact cggtgttgca
    15061 tggcatctct tcggccatgc cgtcgttgat ggcggcgtac aagatcagtt cgcgggcggc
    15121 ccaggtgggg ttcgactggc ccaatatcga gggtcttttc gacaaactcg aagaagaaac
    15181 cgatgaactg cgcgagaatt tagaggagta tcccgagccc ggaccgcgac cagtcggggc
    15241 gtcttccacg gccagtgcac ggggagtgaa ggtgtcagag gaattacagg cacggctgga
    15301 agacgaagtg ggcgatctgt tgtttgtgct ggtgaacgtc gctcgctatc tggcgctcga
    15361 tcccgagtcg gcactgcgca agacgaactg taagtttagg cggcgatttg aatatctgga
    15421 agacaggctg cgagagcagg gtcgcaaacc gtctgatgca tcgctcgatg aaatggagtc
    15481 gctgtggcag gagtcgaagc agcaggaaaa gccgtgaccg tggagactct ccggctgcaa
    15541 aattgctgcg agctggaaga gtttcgtgcc tgcgtcgcat tgcagcagga agtttggggc
    15601 ttcagtgacg cggagctggc acccttacgc attttctcgt tagctccaaa gattggcggc
    15661 caggtcgttg gtgcttggga aggaaattcc ctagtgggat ttgcgatggc cattccaggt
    15721 agccggaatg gtcggccgta tctgcactcg cacatggtgg cggtgaaaga gggccatcgc
    15781 aataccggac tcggacgaaa aatcaagctt ttccagcgcg aagatgccat agcgcatgga
    15841 tacgagttga tggagtggac gttcgatccg ctggaaatca agaacgcgta tttcaatctc
    15901 gagcggctgg gcgctattgc ccgacgttat aacgtcaacc agtacggaat cacgaattct
    15961 ccgctgcagg gatttctgcc caccgatcgc ctggttgcgg aatggtggat gacatcgcga
    16021 cgggtacaga cggtgctgtc gagtggccat cattcgccaa ttgaagagga agaacgaatc
    16081 gaagtgccgg gcgagattta cgcatggaag gccgaacccg caatgcgatc gagagcagaa
    16141 cagattcagt cgagaaaccg tgaacacttt cagtcggcat tctcccgcgg tctggcatgt
    16201 ctcggttacg agcgcgatgc ggatgggaat ggctgctttc aacttggtcg ctgggacgaa
    16261 gactggtcgt atgctgcaaa ttcgtagtgc aagacggtac gcatgaagat cgaagcgatt
    16321 accctcctcg agctgcagat tcccctagtg cattttttcg agacgagttt tggccggact
    16381 tacaccaggc gcgtattgtt gttgacattg cactgtgacg gtctggaggg ctggggcgag
    16441 tgcacggccg gcgaggaccc ttactactcg gaggaataca tcgagggcgc ctgggacgtg
    16501 atcgttcgct acctcggccc atcgttgatt ggaaggacgc tgagtgccgg tcgcgaggtg
    16561 ccagcgctac tggccaaggt gcgcgagaac cgcatggcca aggcagcgct ggagaatgcg
    16621 gcgtgggatg ccgaggcgca ggagaaggga atcccgcttt ggaggctgct aaacggtacg
    16681 cagcgtgaag tcgcttgtgg cgtcagcatc ggcattcagg attcgcacga ccagttgatg
    16741 caaaagatcg agacggaaca cgccgccggg taccaacgca tcaagcttaa agtgaagcca
    16801 gggtgggacg tggatgtcct ggcgaaggtg cgtgcccggt ggcccgacat tctcttgagt
    16861 tgcgatgcca attctgccta taccctcgac gacatggagc acttgaaaac gtttgatcgt
    16921 ttcaagctgc tgatgatcga gcagccgctg tggagcgacg atttctattt ccacgcgcgg
    16981 cttcagaagc aactccacac ggccatttgc ctggatgagg ctattcgcag ctcgcgcgat
    17041 gcagaggctg cattggaact tggggcctgc cgaatcgtaa atgtcaaagt aggacgagtc
    17101 ggcggattaa cggacgcgat tgcagtgcat gacgttgccc agcgctttgg cgtgccggtg
    17161 tggtgcgggg gcatgctgga atcgggtgtg ggccgctcac ataatgttgc actctcgacg
    17221 ctgcccaact tcaagctgcc aggggacgtt tcggcctcga agcgttactg gaaggaagac
    17281 atcatcgaac ccgaggttga agtgtcgccg cagggaacca tcgccatcac cgataagccg
    17341 ggtagagggt acgaactgcg cagggacttg atcgagaggc tcaccgtccg aaaggagacg
    17401 ctgagggccg catcctttta gatgcagcgc aaagtcattg ccgaactgct ggacgacgat
    17461 ctgggaactt ccgacgagat cgccaagtcg ctggtggatt tgaggcatat caacaactgg
    17521 tttggcggaa cgcggacgac gacagcgctg ttgcggcgca ttgccagcgc gaccggcgaa
    17581 acaaagcttt cgctgttgga aattggggca gggcgcgggg acttgccggt tcaagcgtgc
    17641 agcaggctcg ctgccgcaaa catcgaactc cgggtcacgc tcgtcgaccg catcccttcc
    17701 catttaccac ggaatacagt caactcagtg gccgccgatg cgttgcattt gccgtttcga
    17761 gacgagtcct tcgatgtagt gagttgttcg ctatttgtcc accatttcga acctgacgag
    17821 ctgccacttc tggtcaacga agccctgagg gtttgcagca aagctgttct gattaacgat
    17881 ctgattcgca gccgggtaca tcttggtttg gtttacatgg gcctaccgtt gttcgcgagc
    17941 ccgatcacgt ggcatgacgc gcccgcgtcg gtgaagcggg cctacacctt ggatgagatg
    18001 gaatccatgc tggcgaaatt gccagggaaa cggctggaga taacccggca ttacctctac
    18061 cggatgggcg ttctgttgtg gaaatgaagc gttgcgattt cgacattgcg gtgatcggcg
    18121 ggggacccgc gggtgcgtcg gcggcgatca ccgcggcgcg tttcggcgct agcgttgcgc
    18181 tcctcgaagc gggcgaattt ccccggcaca aggtctgtgg tgagtttgtt tctgccgagt
    18241 cactcgatgt tctgcgcgat ctactgcgat cgcaatctat tgcggctccg gtgttgtgcg
    18301 acgcccccgt catcaaccgc gtgcggctcc tgctgggtgg gcgggttatc acggcacgcg
    18361 ttgtcccgcc cgccctgagc attgcgcgct acgatctgga cttcctgtta tggcaggcgg
    18421 cccaggaagc cggcgtccac acccaatcaa cctgcgaggt gcgcatgatt caaggagacg
    18481 gccccttcga actcgatact tgctccggtg ctattcatgc agcatcggtg atcgtcgctg
    18541 ctgggcgttg gtcgagattc gctccgcgct ctccgctccc ggggggtccc aagtggatcg
    18601 gcctcaaagc gcatttccgc gaagaccgtc cgccccgctc taccgaccta tatttcttcg
    18661 accatggcta ctgcggagtg cagctcgttg acgacactac cgtcaacgct tgcgcgatgg
    18721 tgcgctccga ccgcgctacg tcgctggacg aagtgtttgc gctgcatcca gcactggcgg
    18781 aacgcagccg aaattggcgg gcgatgacgg agccggtaac gaccgcaccc ctgatctatc
    18841 gtgcgcctca accggtcaaa gacaacatgt tgttcgctgg agacgcggct gcattcatcg
    18901 atccgttcgt gggcgacgga atctcgatcg cgctgcgcag cggacgaatg gcagcgatga
    18961 gtctgcgggc atttctcaac agaggaagct cgctggatct tgcgctgacg gattatgaga
    19021 acagctacga acggcagttc gttcccctca tcgcggcagc ctcccggatt cgcggggtac
    19081 tttcgtggtc agcgtcagtc cggatgattg cctttgaatt cttaagggtg cccggcctaa
    19141 tgccgtatgt cattcgcaag acgcggcgcg ccgcttcttg aaggctgcct taccgaatca
    19201 ccaggacttc acgaatgttg tcccgaccca gaaaaaactt gatcgagttg tagtcgtgga
    19261 acagattctc gacatggcgg ttattgaaga ttcgctgcag cttgtaggga ctggcgggct
    19321 gcatctcgat ggtgtctctc gccgcgatgt tgtagcggta gaaaggggct tccggtccgg
    19381 cgtccttggt gtggaaaaaa gaaaacaaaa ttgcgctggg cttcatcgca acgtggatgc
    19441 gctcgattac cggtttcact agtgcctcgg gcaggtagtc ggcgaggtcc caaaacatga
    19501 ccgcatcgaa ctcctgccgc tcgaagcaaa ggttctcacg gaggaagcgt tcgacatcaa
    19561 tggtcttttg accctcactg ttagagacga tgagcgactt atccgatgag aggcgcagga
    19621 catcttcctg gtggaagcgg tgtccaagcc caataaaaaa ggttatgttc gcgggtgatg
    19681 tgggccccag atcaaggacg ctgagcccct cttcccgggc cagccacttc ataaactcgg
    19741 taaaaccagt cgagcggcgc tgcacgcgct caccggacac aactccgtct gccgagtccg
    19801 actggccaga accaccgaat atcctgctga acatgcttgg cgacactggg cactattgta
    19861 agggcagcgc gacgcaagcg ccaatttcgc gcgaattgcc gaaggatttg ttgcaggcgg
    19921 gtgcagcggc tgttccgacg aaaggcctga tcgagtccaa aaagaagcgg gacgctgggg
    19981 cgcgtcccga caagattgtt tgacagcaat tagaactttt tgggttcgag caaggatccc
    20041 tgtgtggctg gtgccgcgac cgggatgggc gccggaactg gggtgggcgc agccgctacc
    20101 accgccgccg gcttgggttc gagccgaggt gccggctcgg ctttttgtat gtcgatggaa
    20161 cctttgaaaa aggcgccgtc ttcgatggcc acacgcgccg tcgaaatatc gcccgacaac
    20221 gacgcgctct tgcgcagttc cacgcggtca ctggcctgaa tattgccgtc cacccggcca
    20281 tgcacgatga cgttgcgagc ttcgatgtta gcgcgaaccc gtccgttagg cccgatggtg
    20341 agactctggc cacgcagcga aatcgagccc tccacttcgc catcgacgta gaggtcctcg
    20401 cttccagaca actcgccctt cacaatgacc gactttccga tggtggcgac gtcaacgctg
    20461 cgagggacgt cggagcggac tggttccgcc gcccggactg caggcggggg tggcgtgtat
    20521 gccggcttgg gagcaggttg ttcaggtgcg ggtgtcaaga attcatcttc cttccgcgtt
    20581 gatttccaca taagtcataa ctccgtgaaa catttcgagc gacggcgcag ctgcccccag
    20641 ggcagtctta aatcgccctc aatccactct caagaggata cagaaggatt tgcgcgacgg
    20701 ccaaccctta ttccacagga cgacctcgtc cgggcattga ctatccagcc accgcccgtg
    20761 gctcgccgcc cctcaacggt ctgtccgcac aaggaggtag gctcccttat aattaagggt
    20821 ctcgcaagtc tcccaccgcc gcgttaccag tcgcggaaca agcgcctgag ccctgaagcc
    20881 ggtgctgagg gattttttgc acggacagtg gttgccgttt gattcggcgc tccggccgaa
    20941 caatctgtct tccaaaggct gggaagcaaa tccaacggtg cgggacgagg actcataact
    21001 tatgacccaa atcgaaatta ttattgc
//
LOCUS       DASRKK010000538         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_730597, whole genome shotgun sequence.
ACCESSION   DASRKK010000538 DASRKK010000000
VERSION     DASRKK010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730597"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..770
                     /locus_tag="VFC15_12325"
     CDS             405..770
                     /locus_tag="VFC15_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10984.1"
                     /translation="MIVEPVVHVDPEAELQSAIAALVKITETVETVRWIEFPASVLLF
                     LLVPGDPDSGAVYVLDRKKGTWYAVDFEDEEFGGYSVSQLEMLLKECNFLDLIERPGL
                     WRAGLQWVVLPGMRPESAV"
     gene            882..1856
                     /locus_tag="VFC15_12330"
     CDS             882..1856
                     /locus_tag="VFC15_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10985.1"
                     /translation="MATTITATRQVVEDYQRLFVNRRAYTMQSPRPHPETGRHYYFRP
                     SRKGSDIPLMLTEGTIRQHLEGTITVGLYAINPSTQHCKWVAIDADYRNSMEDLLKLQ
                     YRLTQDNVEPALEMSKRGGHLWIFLATPLLARECRIYIHDLALRLGVPVKGAGLAEGI
                     EVFPKHDAIGAGEFGNAIRGPLGIHRGANRRFWFYGADYTLEDQIAYLKRLRKVTEEE
                     LRRFIAGKARPESDTPSRAQRNIRPEPKEGTARSEFRILDHVGTVRKVGRNYVARCPS
                     CADCGHDRSGDNLAILIEDPRFYKCWAGCTKEMIRAALGCPIRVRHSA"
     gene            1878..2519
                     /locus_tag="VFC15_12335"
     CDS             1878..2519
                     /locus_tag="VFC15_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10986.1"
                     /translation="MKQKRLPDALGLRLTRQALRVLKERGIFAHSPVSLEHQHLAKRY
                     VVRGLESGGAVGDLGRYVTFTTEEGRPIEWLHPVEAIGVNGLHAVVVAPVLVRVEMLR
                     KGRTYELLITQHRPGATNDGRRPPLETRLLSRGIHGRLDLDLSGKDKDKAGTVLPTFY
                     SRAGEEVRIPKRFRPVVRAIVGAVHCNGCFHCHFVRPPQKEEEQVCAAERATA"
BASE COUNT          612 a          748 c          805 g          541 t
ORIGIN      
        1 cggttggtcg gctactcgaa agtaatccag tttgacggtt tgtcgccaaa ccgttgaact
       61 gccactcgcg tgcaaactcc cgagataccg gcctatggga gatgccgaac cgctgcacca
      121 gcggcattgt tggtggctca cacattacct ttcggcgcca tcgcggagta gtgatcaatc
      181 tgagcgtgcg acttgcatcc tttatatata gagatcgtcc tgcttagcga ttttcattct
      241 gctgtcaccc accctcaacc ctagtactgc caacaaaaaa agacacttaa cgcactttac
      301 cctcctttgc caactgacgt gtgcgccatc ggtcgcgtct tggctttcaa cacaacacga
      361 cttgggcgca ctgcccggaa aggacaaccg cgatgttcgg acaaatgata gttgaacccg
      421 ttgtgcacgt tgaccctgag gccgagctgc aatcagcgat agccgcattg gtaaaaatta
      481 ccgaaacggt cgaaaccgtc cgctggatcg agtttccggc gtcggtcctg ctgttcttgc
      541 tcgtacccgg agatccggat tctggggcgg tctatgtcct agaccgcaag aaaggaactt
      601 ggtacgccgt cgacttcgag gacgaagaat tcggcggata cagcgtaagc caactcgaga
      661 tgctcctgaa ggagtgtaac ttcctcgatc tcattgagcg gccgggactt tggcgcgcag
      721 ggttgcagtg ggtggtactg cccggaatgc ggcctgagag cgccgtttga cagaaaaatt
      781 attccgggtt atctaacgac acccacatcc gaaaaccgtg tgggtaaatg gcgaacacga
      841 gccgtcgggc tcgcgaaatt gccagaagga ggtgacggtt aatggcaacg acaatcacgg
      901 caacccggca ggtcgtcgag gattaccaaa ggctgttcgt gaaccgccgc gcgtacacga
      961 tgcagtcccc gcggccgcac ccggaaacgg ggcggcatta ctacttccgg ccaagcagga
     1021 aaggaagcga catacccctg atgctgaccg agggaaccat ccgccagcac cttgaaggca
     1081 caatcacggt cggcctgtat gccattaacc cttcgaccca gcactgcaaa tgggtcgcca
     1141 tcgacgccga ctaccggaac tccatggagg acttgctgaa actgcagtac cgcctgacac
     1201 aggacaatgt ggagccggcg ctggaaatga gcaaacgcgg gggccacctt tggatctttc
     1261 tggcaacgcc gctgttggcc agagagtgcc gaatctacat ccatgatctt gccctgaggt
     1321 tgggagtgcc ggtgaaaggc gcgggcttgg cggagggcat cgaggtgttt cccaaacacg
     1381 acgccatcgg cgccggagag tttggcaacg ccattcgtgg acctctggga atccaccgag
     1441 gcgccaaccg tcgattttgg ttttatggcg ccgattacac gctggaggac cagatcgcgt
     1501 acctcaaacg tctgcgaaaa gtcaccgagg aagaactgcg gaggtttatc gccggcaagg
     1561 caaggcccga gtcagatacg ccgtcccgtg ctcagagaaa tattcgacct gaacccaaag
     1621 agggtactgc tcgctcagaa ttccgcattt tggatcacgt gggtaccgtg cgcaaggtcg
     1681 gccggaatta cgtcgcacgc tgtccatctt gtgcggactg tggccacgac cggagcggcg
     1741 acaacctagc catcctgatc gaagacccac gcttttacaa atgctgggct ggctgcacca
     1801 aagagatgat tcgggccgcc ctcggctgcc cgattcgcgt acggcattcc gcctgagaca
     1861 aggagagtgc tgttgtcatg aagcagaagc gattgccaga tgcactggga ctgagactta
     1921 cgcggcaagc gttgcgcgtg ttgaaagaac gaggcatctt cgcccattcg ccggtaagcc
     1981 tggaacacca gcaccttgcg aaacgctatg tggtgcgcgg gttggagtcg ggcggagcgg
     2041 tcggagacct cgggcgatac gtcacgttca ccacagagga aggccggcca atcgagtggc
     2101 tgcatccggt ggaggcgatc ggagtaaatg gcttgcatgc agttgtggtg gcccccgtcc
     2161 tggtgcgagt tgagatgctc cgcaagggac gcacatacga actgctgatt acgcagcatc
     2221 gaccgggtgc gaccaacgat ggaaggcgac cgccgctgga gacaaggctt ctgtctcggg
     2281 gcattcacgg tcgcttggat ctggatttgt cagggaagga caaggacaaa gccggcacag
     2341 ttctgccgac gttctattca cgcgccgggg aggaagtcag gataccgaag cggtttcgcc
     2401 cggtggtacg cgccatagtc ggtgcggttc attgcaatgg ctgtttccat tgccactttg
     2461 tcaggccgcc gcagaaggaa gaggaacagg tttgcgccgc agaacgagcc acagcctgac
     2521 atggctgaac ggaaggagtg aatagcgatg catagcaagg tcaaaaacta cgcgctggtt
     2581 ttctgtctgt ttgtgttttt gttcgtaacg atcggaggcg gcctgcacat gaacccgggc
     2641 ggtgcattcc ttgccgcagc gaccggcacg ccactgatga tttatgcctt caatcgcaag
     2701 tccaaa
//
LOCUS       DASRKK010000539        19186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_339284, whole genome shotgun sequence.
ACCESSION   DASRKK010000539 DASRKK010000000
VERSION     DASRKK010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339284"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFC15_12340"
     CDS             <1..354
                     /locus_tag="VFC15_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10987.1"
                     /translation="STPEITLGARRPGSLPANQESVFQLKAGEISQTFNDTAAAYIYK
                     VVTVRQIPLSDEKDSIVKTLQQQQVQEKLDAIGKSATPVLNDEYFGPPPTAAAPATGG
                     RPGPSGPPQSANPPQ"
     gene            359..1411
                     /locus_tag="VFC15_12345"
     CDS             359..1411
                     /locus_tag="VFC15_12345"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM10988.1"
                     /translation="MTQPTATVVVTGIAGNLGTRLLPMLSEFRVVGVDVRPPEASQPM
                     EFHAINLGHESSCRELTQVLRHTGARSVVHLAFVLDPQQAGVLDVERMWQINVAGTAR
                     VMEAISVVNRTGGHVRQFIFPSSVSAYGPETPGPVMEDFPLRAHTLPYAIHKRESDEV
                     VRYRAESMGDCRTYILRPQIFTGAAMQDYMVGALRGIPLGNSPRAARMRAEGKRLPLM
                     LPRGKKYDDRRFQFVHVDDVARLLVYLLHRPHSDPAVTILNVAAHGEPLSIQTCAEIA
                     HAKITRVPNRAAYRLVLRMLWKWGLSSIPPEALPYLIGSYTVNTDRLQQFLGADYSQV
                     MHYSVEDALRDSFRHP"
     gene            complement(1449..2240)
                     /locus_tag="VFC15_12350"
     CDS             complement(1449..2240)
                     /locus_tag="VFC15_12350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HZM10989.1"
                     /translation="MNSVKPEGVGHPTTGDTDGRVVGAAPVGASDEASAAVAVRDMFS
                     SIASKYDFLNHALSMNVDRLWWNRTARMFDSILSRPNAQVLDLCCGTGDMTFALYRRM
                     KGASYGLVGADFSHPMLLRASEKTGERRIRWVEADALNLPFPSGQFDLVTAAFGFRNL
                     VNYDRGLAEIYRVLADGGEIGILDFGEPQGLIGAVYRVYFRHVLPAIGTVISGVSGPY
                     AYLPRSVQRFPAPEEMLQRMRLAGFREVSWTPYTFGIAGLYRGKK"
     gene            complement(2237..3289)
                     /gene="aroB"
                     /locus_tag="VFC15_12355"
     CDS             complement(2237..3289)
                     /gene="aroB"
                     /locus_tag="VFC15_12355"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZM10990.1"
                     /translation="MERGLLRSASALLRELVPSHAKAFLVSSPPILRLWGTSLTESFS
                     DAGVSLEVLEMTDGETSKRLSAVEKLAGKMVSLGADRRSVVIAFGGGVVGDVAGFLAS
                     IFMRGIPVIQIPTTLVAQVDSAIGGKTGVNLVSGKNLMGTFHQPLAVLADPEVLSTLP
                     DREYRSGLFEAMKYGVIRNPQIFDLMETHREALLRRDGELLERLIVECVQVKADVVSA
                     DEREGGERRILNFGHTIGHALESATKYKHFLHGEAVGWGMVAATLIGEGLKITDPGAA
                     QRIIAMVLAYGSLPVVQVDGKRILTLLQSDKKTMGGVPHFILPTAIGKVDVVNTVSGR
                     AVIEAAEEISQLSKAR"
     gene            3507..5078
                     /gene="nadB"
                     /locus_tag="VFC15_12360"
     CDS             3507..5078
                     /gene="nadB"
                     /locus_tag="VFC15_12360"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZM10991.1"
                     /translation="MKALPGETDFIVVGAGIAGLRASVQLAPAGRVLCLAKREVTESN
                     TQYAQGGIAAALSDDDEVSLHLDDTLKAGDGLCNEEAARILVEEGPARIEELIEWGTE
                     FDRTGSKIAFTREGAHSRNRVLHAHGDSTGREIGRALYLKAKALKPIEFREFEFTTEI
                     VVRDGRAVGVRVLDNNAQLHEIHASSVLLATGGLGQVYSDTTNPRVATGDGVAMAYRA
                     GADISDMEFVQFHPTALYLKNAPRFLLSEALRGEGAYLRNVELQQFMHKYHEMGELAP
                     RDVVARAIAHELEVTKRPDAVVYLDLTHLKSETVRKRFPTIYATCMQYNVDITEELVP
                     IRPAAHYAMGGVRTDLDGRTSIPGLYAAGEVACTGVHGANRLASNSLLEGLVYGARAA
                     RAMRNEALSSHASVGAATKSAVQTDGSAAETEKFIQKVQTTMWQHVAVVRDGKVLKQV
                     VADLTAMHQQLPKSGDRRSHEAANILQTGLLIARSALAREESRGAHYRLDFPLKNDRK
                     FAKHSVVKGDAISFE"
     gene            complement(5094..6050)
                     /locus_tag="VFC15_12365"
     CDS             complement(5094..6050)
                     /locus_tag="VFC15_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZM10992.1"
                     /translation="MPSNWIFWVLFNVFVLAMLALDLGVFHRKKHVVEFREAIGWTMV
                     WITLAAVFAVLIYFFGHTMTGHHARPNSVLALEFVTGYLIEESLSVDNLFVFLVIFRY
                     FAVPRRYQHEILFWGVVGALVMRAVFILVGIKLLNAFEWIIYVFGAILIYSGVKLFRQ
                     QDDSIDPERNIVLRWFRKLFRVTDKYEGDNFFIVRSGIRYATPLFVVLLLIESTDLLF
                     ATDSIPAVLAITREPFIVFTSNVFAILGLRSLYFALAGMMEVFHLLHYGLSVILIFIG
                     AKMLVSHYVRIPIGVALAVVAGVLAISIVLSLAFPKKAEIAG"
     gene            6255..6782
                     /locus_tag="VFC15_12370"
     CDS             6255..6782
                     /locus_tag="VFC15_12370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM10993.1"
                     /translation="MARQPGVYATLDTSEGTIVCRLFEQEAPITVKNFIELAEGTRDW
                     TDPRTRQKSNSPLYDGTIFHRVIPDFMVQGGDPTGTGMGGPGYQFQDETKGSPHKFDK
                     PGKLAMANSGPGTNGSQFFVTVAATPWLTGNHTIFGEVVEGQDIADKITAVPRGRQDK
                     PNKDVVLKSVKIERV"
     gene            complement(6896..7117)
                     /locus_tag="VFC15_12375"
     CDS             complement(6896..7117)
                     /locus_tag="VFC15_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10994.1"
                     /translation="MEFLCSVCGRREGEPQGWHLVIEMAKPGSNVRNTMFILDRWDGK
                     RAHDPNAASFCSTACKDKYLAERHRQLFA"
     gene            7487..8632
                     /locus_tag="VFC15_12380"
     CDS             7487..8632
                     /locus_tag="VFC15_12380"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZM10995.1"
                     /translation="MMNCNTCGREIAAGTVCPYCGAHNPVAPGITTPGVLVSRRAVGT
                     EISATHVIVGINVAVFLGMALSGASLLNPSRELLLRWGANWGPLSLGTQPWRMLTSNY
                     VHIGLIHILFNMWCLWNLGQLAERVLGRMNYVVLYTFCGLAGSLASLWWHPLSVGAGA
                     SGAIFGLAGAAIAVFYLGHLPIAKEALSGTMRSLLTFVGYNLFFGLTPGIDNSAHIGG
                     LVAGLLMGAALSKHIMVAPDVRRQWARLTWIAMAVVLLLGNVAVRRQYPQLAQLANPQ
                     VIQAQQLANARRAMLQHRTDDAIAQLQEIIQQQPKEAEPRYLLGEAYLYQHKPDDAIA
                     AFREALRLKPDYAEAEAALGSAYLDKGMKPEAEQAFKKAAELGYTGD"
     gene            8667..9293
                     /locus_tag="VFC15_12385"
     CDS             8667..9293
                     /locus_tag="VFC15_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM10996.1"
                     /translation="MKKWMLLFTLVLVGAIIASGKSTKLVTSWKNPEYSGPRLHKILV
                     LGMSDKPGVRADFEDALSKLVGGNGVEAIPGNTILLRPEDTKLDLNYLKAQIREFKID
                     AVIVSRLVKVETNVTYIPGQPYMMPFPYYNSFYGYYGAVYPVVYSPDYLREDKTVRVE
                     TNVYDTRTPDAQIVWTGISDTFNPRSADKAIDGVTKLIVKELQKEGVL"
     gene            complement(9406..10332)
                     /gene="mdh"
                     /locus_tag="VFC15_12390"
     CDS             complement(9406..10332)
                     /gene="mdh"
                     /locus_tag="VFC15_12390"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZM10997.1"
                     /translation="MRKKVTVVGSGNVGATAAHWMAAKELCDVVLIDIIDGVPQGKGL
                     DLLEAMPIEKRDSFVIGTNDYADTANSDIVVITAGIPRKPGMSRDDLLNTNYKIMSDV
                     VGKAVQYSPNCILIIVSNPLDAMAQAAYKLSGFSRNRVIGMAGVLDSARFRTFIAQEL
                     KVSVENVTAFVLGGHGDTMVPLSRYSTVAGIPITELIEPTKLEQLVQRTRDGGAEIVK
                     YLKTGSAYYAPSAAVTEMVEAILKDKKKILPCAAYLEGEYGINGLYVGVPCKLGSAGI
                     EEIIQIKLTADEQAALQKSADSVKELVGVIKV"
     gene            complement(10439..11866)
                     /locus_tag="VFC15_12395"
     CDS             complement(10439..11866)
                     /locus_tag="VFC15_12395"
                     /inference="COORDINATES: protein motif:HMM:NF005610.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZM10998.1"
                     /translation="MAASYNGIPLPNSGSKITFENGKLVFPDKPIIPFIEGDGTGRDI
                     WKASVRVFDAAVEKAYGGKRRVAWYEVFAGEKAMGRFKTWLPDETVNAFRDLRVGIKG
                     PLTTPVGGGIRSLNVALRQILDLYGCVRPVKYYKGIPSPVKNPERMNIVIFRENTEDV
                     YAGIEWRQGTPQAKKLIDFLNNEMLAGTNKHVREDSGVGIKPISVFGTKRLVRMAIKY
                     ALENGRKSVTIVHKGNIQKFTEGAFREWGYELATEEFRDHVVTERESWILDNVDKHPG
                     LSIEDNAKMFEPGLEFAQQDFRDALYKEVKGAIDSIYATHGRGAWKKKLMINDRIADS
                     IFQQIVTRPDEYAVLATPNLNGDYISDAAAAQVGGLGIAPGANIGDEHAIFEATHGTA
                     PKYADKDVINPGSVILSGVMMFDFMGWHEAARLIEGSMEHTILQKKVTYDFERMLEGA
                     TKVKTSEFATHMIHNMEPIAELHAE"
     gene            complement(12092..15025)
                     /locus_tag="VFC15_12400"
     CDS             complement(12092..15025)
                     /locus_tag="VFC15_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HZM10999.1"
                     /translation="MTENRQSGAVQLPSDIEFDDPVAATKNLSRLREVVSESVFAAFL
                     QFLTTSPSPDSVVVQFERLMEAAPREMAAAFEKQPILIHYATLVFGYSAWLGETLIQN
                     IELLRRFGRNKSLDRSCSREEFREEFARMRSRSPDSDMAVSLARFRKREYVRILLRDV
                     LGIAKLAETTEEISALSDALIDEALLAVNSALQHRHGTPLRVDPQGRLRDSRFAVLSL
                     GKLGGNELNYSSDIDLMFLYDGGVEPPSQGISNREYFIQLAQQTTELLSRPTGEGQVF
                     RIDLRLRPQGHEGEVALALPRAAQYYSQVAQDWELQAMIKARHSAGDADLAREFIRDV
                     APHVYRPNVNFAAVKTALQSRERFDKRGNKARPGRSGRRAIDVKLDRGGIRDIEFLVQ
                     CLQRVYGGEEGWLRSRATLFALQKLHDKEHISGKDYDNLTSAYEFLRHLEHLLQLRQG
                     RQNHQLPSSPMELRALAKCLNREGSTAPAPDEFVRQIESRMATVAEIYRRIVYQEQSQ
                     QFIDAEGNLRLQAQVSASAENSYSQIMQRLAVDAPRLLAVISHANLPPQGRRNLDRFL
                     SSATTNSQRYGAVLRSPGAVERALTVFEFSNYLTDILVRYPEDVALLDDIKEQPEPAA
                     AALFAAPQHQGNSIPNPISDHPALSSVDRHDALALLRQRARQALFVSGARDLYFSRSV
                     FEALEENTQAANRALQSALAIAGPPPGFAVMALGRLGSREFDLLSDADVLFVADEATN
                     PEVARRAAERTVESLTAYTRDGTVLPVDARLRPQGREGELVTTAAQLARYFAGAARPW
                     EAISYLRLRFVAGDPVVAGLALASIREGIADMAERPDFARELTDMRARLESSDSAPNS
                     KAWPGGEYDIDFIAGRLQAKHRIWSQGNLSERLSLLREHGHLGEREYRELAASSKFLR
                     TLEHFVRLVTGRPGKWLPAGDYAQTCVARLMARLPGRDESLTLEEDLATVLRRTREIY
                     LEHMF"
     gene            15201..16511
                     /locus_tag="VFC15_12405"
     CDS             15201..16511
                     /locus_tag="VFC15_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZM11000.1"
                     /translation="MHWLNALFGFDRHGTTVTREVVAGLTTFTTMSYIVVVNPAILSA
                     AGIPAGPSFVSTVLVAVAGCYLMAFYANRPFAIAPYMGENAFIAFTVCRMLGFAWQTA
                     LAAIFIAGVLFVVMTVLRLRQWIVEGVPTCLRYSFAVGIGLFLTFIGLNQTGLVALGV
                     PGAPVQAGHLTSHPALVAMFGFVLLAVLTIRKFPGAILVGIIVTAIVAFLAGTAAPPH
                     HLVSLPPSLSPIFWQLDFRGALTWRAFPVALTIFIMAFVDTMGTLIGLSARAGFLDEN
                     GQLPQIERPMLVDALTTCLAPALGTTTAGAFVESATGIEAGGRTGFTVLITGTCFLLT
                     LFFSPFVAAIPAQAYGPALIIVGLFMLSPVTRIDFSDYSESIPSFAVVVLMAFTFNIA
                     IGISAGFVLYPICKLVAGKTAQLKPGLWVLTSLSLLFFVFYPYS"
     gene            16923..17597
                     /locus_tag="VFC15_12410"
     CDS             16923..17597
                     /locus_tag="VFC15_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM11001.1"
                     /translation="MAAHHRNTASSLAQESQIAISRVALYARVSTLNGQDPEMQLSEL
                     REYASRRGWSITREYVDQGVSGSKESRPELNQLMADAHRRKFDAVLVWKIDRFGRSLK
                     HLVNALADLCAYGVAFISFRDNLDLSTPSGRLMFQIIGAMAEFERSLIQERVRAGLRN
                     ARAKGKKFGRPRAQVDADRVAALRQEGLSWSQVCRALKVSKGSAQRSVARLPNRDKTG
                     LLASCS"
     gene            17651..18709
                     /locus_tag="VFC15_12415"
     CDS             17651..18709
                     /locus_tag="VFC15_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM11002.1"
                     /translation="MKLLERSFVVVLALLAALAVADAAQSGPADNAALPDPGPIYGAR
                     LEGFDYPWPVALYRFQSQGQALEMAYMDVKPARPTGRVAVLLHGKNFCAATWQTTITA
                     LVEAGYRVIAPDQIGFCKSSKPAQYQFSFQQLAGNTHALLESLGVERATVIGHSTGGM
                     LGIRYALMYPSQVDQLVLVDPIGLEDWKARGVPWQSVDAWYQRELQTTADRIRAYERA
                     TYYAGTWETNYDRWVQMLAGMYRGAGRAQVAWDSALLYDMIYTQPVLYDFGALRMPVL
                     LMIGDKDTTAIGKDLASPEVRAKLGNYPVLGKAAAARIPHAKLVEFPDLGHAPQIQAP
                     ERFHKARLEGLQAPSTYQ"
BASE COUNT         4008 a         5715 c         5461 g         4002 t
ORIGIN      
        1 tctacgcccg aaatcacttt gggtgccagg cgtccgggca gcctacccgc gaatcaggaa
       61 agcgtatttc aactgaaggc gggagagatt tcgcagacct tcaacgacac ggcagcagcc
      121 tacatctaca aggtcgtgac cgtaaggcaa atccccttga gcgacgagaa ggactcaatc
      181 gtaaagactc tgcagcagca gcaagtgcag gaaaagctgg acgcgattgg caaatccgcc
      241 acgcccgtgc tgaacgacga gtatttcggc cctccgccca ctgcggctgc tcctgccacc
      301 ggaggccgcc cgggtccgag tggtccacca caatccgcta atccgcccca gtaattcgat
      361 gacacagccg acggcgacgg tggtggtgac ggggattgcg gggaacctgg ggaccaggct
      421 cctgccgatg ttgtcggagt ttcgcgtagt gggcgtggac gtgcgcccac ctgaagcctc
      481 ccagcccatg gaattccacg cgataaacct ggggcatgaa tcgtcatgcc gtgaactcac
      541 ccaggtgctt cgccacaccg gtgcgcgttc cgtggtgcat ctcgcgtttg tacttgatcc
      601 gcagcaggct ggagtgctcg atgtcgagcg catgtggcag atcaacgtcg ccggcaccgc
      661 gcgcgtgatg gaagccatca gcgtcgtcaa tcgcacaggt ggacatgtcc gccagttcat
      721 cttccccagt agcgtttcgg cctacggtcc cgaaactccg ggaccggtga tggaagactt
      781 tcctttgcgg gcccatacgc ttccctacgc gatccacaag cgcgagagtg atgaggtggt
      841 gcgctatcgc gccgagagca tgggcgattg ccgcacttac attctgaggc cgcagatttt
      901 caccggcgcg gccatgcagg actatatggt gggcgcattg cggggaatcc cgctgggcaa
      961 cagtccacgg gcggcgcgca tgcgagcgga aggcaagcgc ctgcccctca tgctgccgcg
     1021 cggcaagaaa tacgatgacc gtcgttttca attcgtgcac gtggacgacg tggcacgtct
     1081 actggtctat cttcttcacc ggccgcacag cgatcccgcg gtaactatct taaacgtggc
     1141 tgcgcacggt gagccactct cgatccaaac ttgtgcggag attgcgcatg ccaagataac
     1201 gcgcgtgccg aaccgggccg cctaccggtt ggttttgcga atgctgtgga agtggggact
     1261 ctcgtcgata ccacccgagg cgttaccgta cctgatcggc tcctacactg tgaacaccga
     1321 ccgcctgcaa caattcctgg gcgcggatta ctcgcaagtg atgcactact cggtggaaga
     1381 tgcgctgcgc gacagcttca ggcacccata ggctcccggc gcaacaaatt actattgctc
     1441 agtgacagct atttcttgcc gcggtacaaa cctgcaattc caaacgtgta gggagtccac
     1501 gacacttcgc ggaaacccgc gagccgcatg cgttgcagca tttcttcggg agcgggaaaa
     1561 cgttgcaccg acctcggtaa gtacgcgtac ggaccgctca ccccggaaat gactgtgcca
     1621 atcgctggca aaacgtggcg gaagtacacg cggtaaaccg cgccgatgag cccttgaggt
     1681 tcgccaaaat cgagaatgcc gatttcacca ccgtccgcga gcacgcgata gatctcagcc
     1741 agtccgcgat cgtaattgac cagattgcgg aacccgaacg ccgccgtcac caagtcgaac
     1801 tgaccgctgg gaaacgggag attcaacgcg tcggcttcga cccagcgaat tctcctttcg
     1861 ccagtctttt cgcttgccct gagcagcatg gggtgcgaga aatcggcgcc caccaaacca
     1921 tatgacgcac ccttcatgcg ccggtacaga gcaaatgtca tgtcgccggt tccgcaacat
     1981 agatcaagca cctgcgcatt cgggcgcgaa agaatcgagt caaacatcct cgcggtacgg
     2041 ttccaccaca agcggtcgac gttcatcgaa agcgcatggt tgaggaaatc gtacttcgac
     2101 gcgatcgagc tgaacatgtc gcgcacggcg acggccgctg acgcttcatc gctggcgcca
     2161 accggagcgg cgcccacgac ccgaccatcg gtatcgccag tcgttggatg tcccacacct
     2221 tctggtttca cagaattcat cgtgccttcg atagctgact gatttcttca gcggcttcga
     2281 tcactgcgcg ccccgacaca gtattgacca cgtccacctt gccaatcgcg gtcggcagaa
     2341 tgaagtgcgg cacgccaccc atcgtctttt tgtcggactg gagcagggtc aggatacgct
     2401 tgccgtcaac ctgcacaacc ggcagtgatc catacgcgag caccatcgcg atgatgcgct
     2461 gcgccgcgcc cggatcggtg atcttgaggc cctcgccgat caatgtagcc gctaccatgc
     2521 cccacccaac cgcctcgccg tgcaggaagt gcttatactt ggtcgccgat tccaaggcat
     2581 gtccaatggt gtggccgaag ttcaggatgc gccgctcgcc gccttcccgc tcgtccgcgc
     2641 tcaccacgtc ggccttcacc tgcacgcatt caacaatcag ccgctccagg agttccccat
     2701 cccggcgcag caacgcctct cggtgcgttt ccattaagtc gaaaatctgt ggattccgga
     2761 taacgccata cttcatcgcc tcaaacaatc cggagcggta ttcgcgatcg ggtagcgttg
     2821 agaggacttc ggggtcagcg agcacggcca gcggctgatg aaaggttccc atcagattct
     2881 tgccagaaac taggttaacg ccggtcttgc cgccgatggc agagtccact tgcgccacca
     2941 gagtcgtcgg aatctggata acgggaatgc cgcgcatgaa aatagacgcg aggaatccgg
     3001 cgacatcgcc gactacgcct ccgccgaacg cgatcacgac cgacctccgg tccgcgccca
     3061 ggctcaccat ctttcctgcc agtttttcga ccgcactcag tcgcttggat gtctcgccgt
     3121 cggtcatttc cagcacctcg agcgagacac ctgcgtcgga gaagctctcg gtcagcgaag
     3181 ttccccacag ccttaggatc ggcggggacg agacgagaaa tgcctttgcg tgcgacggaa
     3241 ccaactcccg cagcaaggca gaagccgagc gcagcaggcc acgctccacc aacacttcat
     3301 acgatcgcga tgaggtacga actggtatgc gagtcaaact tctagtgtaa cggatgcttc
     3361 cctgacccgc ataaagcatc gcggaaaagc attttcccat tgcgagcatc ggggcatttc
     3421 gagtgaatcg catcgctgtt atgcgtgtta ccatcagcat tggaacggct gattcacgcg
     3481 cgttctgagg cattaagcgc attcctatga aagctctacc gggcgaaacc gacttcatcg
     3541 tagttggcgc aggcatcgcc ggactgcgcg cgtcggtgca actggcaccg gcggggcgcg
     3601 tactatgtct cgccaagcgt gaagtaaccg aatccaacac gcagtatgcg cagggcggca
     3661 tcgcggccgc gctcagcgac gacgacgaag tcagcctgca tctggacgac acactaaagg
     3721 ccggcgacgg gctgtgtaac gaagaggcgg cgcgcattct ggtggaggag ggcccggcgc
     3781 gcatcgagga gttgatcgag tggggcactg agttcgaccg cacaggcagc aagatcgcct
     3841 tcacccgcga aggggcccac agccgcaacc gggtgttgca tgctcacggc gactcaaccg
     3901 gacgggaaat cggacgcgca ctctacttga aagccaaggc gctaaagccc atcgagtttc
     3961 gcgaattcga attcaccact gaaatcgtgg tgcgcgatgg ccgggcggtc ggagtgcgcg
     4021 tgctcgacaa caatgctcag ctgcacgaga tccatgcgag ttcagtgttg ctggcgaccg
     4081 gcggacttgg gcaggtgtat agcgacacca cgaatccgcg ggttgcaacc ggggatggcg
     4141 tcgccatggc gtatcgggcc ggcgctgaca tcagtgacat ggagttcgtg cagtttcatc
     4201 ccactgcgct gtatttgaag aatgcacccc gcttcctgtt gtcagaggcg ctgcgaggcg
     4261 aaggagccta cttgcgcaac gtggaactgc agcaattcat gcacaagtac cacgagatgg
     4321 gagaacttgc tccgcgcgac gtcgtcgctc gcgccatcgc ccacgaattg gaagtcacca
     4381 agcggccgga cgcggtggtt tacctcgatt tgacgcatct caagagcgaa accgtgcgca
     4441 aacgttttcc caccatttac gccacttgca tgcagtacaa cgtcgatatc accgaggagc
     4501 tggttcctat ccgcccggcg gcacactatg cgatgggcgg cgtgcgaacc gatctcgatg
     4561 gacgaacctc gattccggga ctctatgctg cgggtgaagt ggcatgtacc ggagtacacg
     4621 gggcaaatcg actggccagc aattccctgc tggaggggct ggtgtacggc gcccgcgcgg
     4681 cccgagccat gcgaaatgaa gcgctgtcct cgcatgcatc ggtcggagcg gctaccaagt
     4741 ccgctgttca aactgatgga agcgcggcgg agactgagaa gtttatacag aaagtgcaga
     4801 caacgatgtg gcagcatgtt gctgtggtgc gcgatggcaa agttttgaag caggtggtcg
     4861 ccgacctgac tgctatgcat cagcaattac ccaagtcggg cgatcgtcgt tctcacgaag
     4921 ccgctaatat tctgcagact gggctgctca ttgcgcgctc agcgctggcc cgcgaggaaa
     4981 gccgtggagc gcactaccgc ctcgatttcc ccttgaagaa cgatcgcaag ttcgccaagc
     5041 actccgtagt aaagggcgat gcgatttcct tcgagtaggt tccccaccct gtattagcct
     5101 gcaatttccg ccttcttggg aaatgccaac gacagaacga tggagattgc cagcactccg
     5161 gcaaccacgg cgagcgctac tccaatcggg atgcgcacgt agtgcgacac cagcattttg
     5221 gccccaatga agatcaggat cacggagaga ccatagtgca gtaggtggaa aacttccatc
     5281 attcccgcta gtgcaaaata cagcgagcgc agacccaaaa ttgcaaagac gtttgacgtg
     5341 aagacaatga atggttccct ggtgatggcc agtactgccg gaatcgagtc tgttgcgaac
     5401 aacaggtccg tggactcgat cagcagtaac accacaaata aaggtgtcgc atagcgaatt
     5461 ccgctccgga caatgaagaa gttatcgcct tcgtacttgt cggtcacgcg gaacagtttc
     5521 cgaaaccacc gcaatacgat gtttcgctca ggatcgatgc tgtcatcctg ttggcggaac
     5581 aacttgacgc cgctgtaaat caggattgcc ccgaagacat agatgatcca ctcgaaggca
     5641 ttcagcagct taattcctac gaggataaac accgcgcgca tcaccagagc tcccaccaca
     5701 ccccagaaca ggatctcgtg ctgatacctg cggggcacgg cgaagtagcg aaagatcacc
     5761 aggaaaacga acagattgtc aacgctgagg gactcttcaa tcaggtagcc cgtcacaaat
     5821 tccagtgcca atacggagtt cgggcgcgca tgatgtcccg tcatggtgtg tccgaagaag
     5881 tagatcagca ccgcgaacac ggccgccaag gttatccaga ccatcgtcca gcctatggct
     5941 tcgcgaaact ccacgacatg cttcttccga tgaaagacgc ccaagtcgag cgccagcatc
     6001 gccagcacaa agacgttgaa gagaacccag aagatccagt tggagggcat cgtttgcggt
     6061 ccgaattgtt tattctaact ggtgggtggg aacccccgga gaattacgag attgcccagc
     6121 ttggacgccg tgttgccaat ggacaacacg catttacgcg taggcaatac aataggcaag
     6181 gcggttctcc accgagcttc actccgctcg gcggcagtgg agctgcccag atatcagcat
     6241 ggaaaggaat tctcatggcc cggcaacccg gtgtttatgc cactcttgat acatccgaag
     6301 gtacgatcgt ctgccgactg ttcgagcagg aagcacccat cacggtcaag aacttcattg
     6361 aactcgcgga aggcacgcgc gattggactg accctcgcac tcgccagaag agcaattcgc
     6421 cgctgtatga cggtacgatt tttcatcgcg tcattcctga cttcatggtg cagggtggtg
     6481 acccgacggg caccggcatg ggcggccccg gctaccagtt ccaggacgag actaaaggtt
     6541 cgcctcacaa gttcgacaag cccggtaagt tggccatggc gaactccggg ccggggacca
     6601 acggttcgca attcttcgtc accgtggcag cgacgccatg gctgaccggc aaccacacga
     6661 ttttcggaga ggtcgtcgaa ggccaggaca tcgcagacaa aataactgcg gtccctcgcg
     6721 gacgtcagga caagccgaac aaggatgtag tgttgaagtc ggtgaaaatt gaacgtgtgt
     6781 agggcgagtt gcagtcaagc gaaaaggccg cgaacgcggc cttttttatt gagacggttg
     6841 caggctcgcg cgaccgtgcc ctcaggtctc gcgaccgatg tcttagtaag gcaggctatg
     6901 cgaaaagctg ccggtgccgc tcggcaagat atttgtcctt gcacgccgtc gaacagaagc
     6961 tggccgcatt cggatcgtgc gcccgcttcc catcccaccg gtcaaggatg aacatggtgt
     7021 ttctgacgtt gctgcccggc ttggccatct caatcacgag gtgccagcct tgaggctcgc
     7081 cctctcgtct gccgcataca ctgcatagaa attccatggt gggagcctcc cttcctatca
     7141 cctgtattga tgcactcatg ctattcctgt cttcccaaga acgtctgtga ccctgggggc
     7201 gaaacgatcg acaactccga agctctgcgg aggtagcgtc acttccgcgg aaaatcgcca
     7261 gccccatctc tcacagctag tgttacggat gcaaccagcg gaaggttcca tcgatatcag
     7321 gcttatcaag ggggacgtga gacttcgcaa aatgtcatac ctttatgtga tgagccgctg
     7381 aggttccgcg accaaatgaa cgagtggcgg cagatctctc tattttgggc agcgcccacc
     7441 gggaggcgcg ctcgcgccca tgtatgctta aagctcggag ctttgcatga tgaactgcaa
     7501 cacgtgcggt cgcgagattg ctgccggcac tgtttgtccg tattgcggcg cgcacaatcc
     7561 tgtcgcaccg ggcattacga cgccgggagt actggtttcg cgccgtgcag taggcacaga
     7621 aatctcggcc acgcatgtca tcgtgggcat caatgttgcc gtgtttctcg gcatggcgct
     7681 ctcgggcgct tcgctgctga atccctcgcg cgagctactg ctgcgctggg gcgctaactg
     7741 ggggccattg tcactgggca cacagccttg gcggatgctc acttccaact acgtccacat
     7801 cgggctgatt cacatcctgt tcaacatgtg gtgcctgtgg aacctgggcc aactggccga
     7861 gcgcgtgctg ggccgaatga actatgtcgt tctctacact ttctgcggcc tggcaggaag
     7921 ccttgcgagc ttgtggtggc atcccctgag cgtgggcgcg ggagcctcag gagcaatctt
     7981 cggactcgca ggcgcggcca tcgcggtgtt ttatctggga cacctgccga tcgccaagga
     8041 agccctctcc ggcaccatgc gaagcctgct gaccttcgtt ggttacaacc ttttcttcgg
     8101 actgacgcca ggcatcgaca actccgcaca tatcggcggg ctagtggcgg ggttgctcat
     8161 gggagcggca ctctccaagc acatcatggt cgcgcccgac gtgcgccggc agtgggcgcg
     8221 attgacgtgg atcgcgatgg cggttgtcct tttgctaggc aacgtggctg tccgtcggca
     8281 gtatccccag cttgcgcaac tggccaatcc gcaggtgatc caggcgcaac aactggcgaa
     8341 tgccagacgc gccatgctgc agcacaggac agatgacgcc atcgcgcagt tgcaggagat
     8401 catccagcag caaccgaaag aggccgagcc ccgttacttg ctgggggagg cttaccttta
     8461 ccagcacaag cccgatgatg caatagcagc tttccgggaa gcgctgcggt tgaagcctga
     8521 ctacgccgag gccgaagcag cgctgggatc agcctaccta gacaaaggta tgaagccgga
     8581 agcggagcag gcgtttaaga aggctgccga attaggctac accggagatt agaaatttct
     8641 tgcgcgactc gctacggaga cagttcttga aaaagtggat gttgctgttc accctcgtgc
     8701 tcgttggagc gatcatcgcc agcggtaagt cgaccaagct cgtaaccagt tggaagaacc
     8761 ctgaatattc cggcccgcgt cttcacaaga ttctcgtgct cggcatgagc gacaagccgg
     8821 gcgtgcgcgc cgacttcgaa gatgctctct ccaagctggt gggtggtaac ggcgttgagg
     8881 cgattcctgg caacactatc ctgcttcgtc cggaggacac caagctcgac ctcaactatc
     8941 tcaaagcgca gatcagggaa ttcaagatcg acgccgtgat cgtctcgcga ctggtaaagg
     9001 tggagaccaa tgtcacctac attcccggtc agccttacat gatgccgttt ccctattaca
     9061 actcgtttta cgggtactac ggtgcggtgt atccggtcgt ctattcgccg gattacctgc
     9121 gggaagacaa gaccgtgcgc gtcgagacca acgtttacga cacgcgtaca ccagatgcgc
     9181 aaatcgtctg gaccggcatc agcgacacat tcaatccccg ttctgccgac aaggcgatcg
     9241 atggagtaac caagctgatc gtgaaagagt tacaaaagga aggcgttcta tagcgaatcg
     9301 gtccgtcggg cggccgccgc ttcacaaaca aatgagcggc atgacatggc aatcgtcgcc
     9361 atactcgcgc cgcccacttc aactaccgat atccttgccg gcgatctaca ctttgatgac
     9421 gcccacaagt tccttgaccg agtcggcgct cttctgcagc gcggcctgct cgtcggcggt
     9481 cagcttaatc tggatgatct cctcgatgcc ggccgagccg agtttgcaag gcaccccgac
     9541 gtacagcccg ttgatgccgt actcgccttc gagataggcg gcacatggca ggatcttctt
     9601 cttgtccttc aggatggcct ccaccatttc ggtcaccgct gccgaaggtg cgtagtacgc
     9661 gctgccggtc ttgagatact tcacaatttc ggcaccgccg tcgcgcgtgc gttgcaccaa
     9721 ctgctccagc ttcgtcggct cgatgagctc ggtgattgga atgccagcca ccgttgagta
     9781 gcggctcagc ggaaccatgg tgtctccgtg tccgccgaga acgaaggccg tcacgttttc
     9841 gaccgagacc ttcagttcct gcgcgatgaa ggtacggaag cgcgccgaat ccagcacgcc
     9901 cgccatgccg atcacgcgat tgcgcgaaaa accgctcagc ttgtaggccg cctgcgccat
     9961 tgcatcgagc ggattcgaca cgatgatgag aatgcagttg ggcgaatatt gcaccgcctt
    10021 gcccacaaca tcgctcatga ttttgtaatt ggtgttgagc agatcatcgc ggctcatgcc
    10081 cggcttgcgg ggaatgccgg ccgtgatgac cacgatgtcg gagttcgcag tgtcggcgta
    10141 gtcgttggtg ccgatcacaa acgaatcgcg cttctcgatc ggcatggctt ccagcaggtc
    10201 gagtcctttg ccctgcggca cgccatcaat gatgtcgatc agcacgacgt cgcaaagctc
    10261 cttggccgcc atccagtgtg ccgcagtggc gcctacgttt cctgagccta caaccgtaac
    10321 ttttttacgc ataaatcctc acttcctaaa gtatttagta acaatgtttt acacacgact
    10381 gtttaagttt tagttcatgt gctgacaaat ccatgctgcc cttctgcaag cgggtgcgct
    10441 actccgcgtg cagttccgca atcggttcca tattgtgaat catatgggtt gcaaattcgc
    10501 tggtcttgac cttggtcgcg ccttccagca tgcgttcgaa gtcgtaggtg accttcttct
    10561 gcagaatggt gtgctccatg gaaccttcga tcagccgggc cgcctcgtgc cagcccatga
    10621 agtcgaacat catgacgccc gacaggatga ccgacccagg attgatcacg tccttgtcag
    10681 cgtacttggg agcggtaccg tgagtggctt cgaagatcgc gtgttcatcc ccgatgtttg
    10741 cgccgggggc gatgcctaat ccgccaacct gcgctgcagc agcgtcggaa atgtaatccc
    10801 cgttcaggtt gggagtggcc agtaccgcgt actcatccgg acgggtgacg atctgctgga
    10861 agatgctgtc ggcaatgcga tcgttaatca tcagcttctt tttccaggca ccgcgtccat
    10921 gggttgcgta gatgctgtcg atcgcgccct tgacttcttt gtatagcgcg tcccggaagt
    10981 cctgctgagc aaactccagg ccaggttcga acatcttggc gttgtcttcg atgctgaggc
    11041 cagggtgctt gtccacgttg tcgaggatcc aactctcgcg ctcggtgacg acgtggtcgc
    11101 gaaattcttc ggtcgccaat tcgtatcccc actcgcggaa cgcgccctcg gtgaacttct
    11161 ggatgttgcc cttgtgcacg atagtcaccg atttgcggcc attttcgagg gcgtacttga
    11221 tggccatacg gaccaggcgt ttcgtcccaa aaactgagat cggtttgatg ccgactccgg
    11281 aatcctcgcg aacatgcttg ttcgttccgg caagcatttc gttgttgagg aagtcgatga
    11341 gtttcttggc ctggggtgtt ccctgcctcc attcgatgcc ggcataaacg tcttccgtgt
    11401 tttcgcggaa aatcacgata ttcatgcgct cgggattttt caccggcgaa gggatgccct
    11461 tgtaatactt caccgggcgc acgcatccgt agaggtcgag aatctgccgc agggcgacat
    11521 tcagcgaccg gatgcccccg cccacgggtg tggtgagagg ccctttgatc cccacccgca
    11581 aatcgcggaa tgcgttgacg gtttcgtcag gaagccaggt cttaaaccgg cccatggctt
    11641 tctcgccggc aaagacttca taccacgcca cgcggcgctt gccgccgtaa gctttttcga
    11701 cagccgcatc gaatacccgg actgatgcct tccagatatc gcgtccggtg ccgtcgcctt
    11761 caatgaaggg aattatcggt ttgtcgggaa aaaccagttt gccattttca aatgtgatct
    11821 tgctcccgct gttgggtaaa ggaatgccat tgtacgaagc cgccactaca cacctcagaa
    11881 agtaggggtc ataaagaaag accctgattt aagaacaaat tgtgtcggaa cagtcgcagc
    11941 ttgccggtga ttggagccat catcggtcac cggaacggcc tatcatggct gcagggcgcg
    12001 cttgcgttgc gcagtgctag gtcttgatta gagagggcaa ccgggctttc ccgaaccttc
    12061 tacgttatca cccgccacgg aagggcttca atcaaaacat gtgttccagg tagatctctc
    12121 gggttcggcg caaaactgtc gccagatcct cctccaaagt aaggctttcg tcgcgtcccg
    12181 gcaatctcgc catcagcctc gccacgcagg tctgggcgta gtcccctgcg ggaagccatt
    12241 tgccggggcg tccggttacc agtcgcacga agtgctcgag tgtgcgcagg aacttcgaac
    12301 ttgcggcgag ctcacgatat tcccgttccc cgagatgtcc atgctcacga agcaggctga
    12361 ggcgttccga caggtttccc tggctccata tccggtgctt ggcctgcaac cgcccggcga
    12421 tgaagtcgat gtcgtactcc ccgcctggcc aagccttgga gttcggagcc gagtcgctcg
    12481 attccagccg tgcgcgcata tcggttagtt cacgggcgaa gtctggccgc tcggccatgt
    12541 cggcaatacc ctcgcgtatg gatgcgagcg caagtccggc aaccacaggg tcgcccgcaa
    12601 cgaaccgcaa ccttaaatag ctgatcgcct cccagggacg ggcagcaccg gcgaaatacc
    12661 tggcgagttg cgcagcagta gtcaccaact caccttcacg tccctgcggg cgcaaacggg
    12721 catcgaccgg caaaaccgtt ccatcgcgcg tgtacgcggt gagagattcc acggtacgtt
    12781 cagccgcacg acgcgcgact tcgggattgg tagcttcgtc cgccacgaac agcacgtcag
    12841 catccgacag caagtcgaat tcgcggctgc ccagcctgcc caacgccatc accgcaaatc
    12901 caggcggcgg acccgcgatc gccaatgctg attgcagtgc cctgttcgcg gcctgggtgt
    12961 tttcctccaa cgcctcgaac acgctgcgac tgaaataaag gtcccgggcg cctgaaacga
    13021 acagcgcttg ccgggctctc tgacgcagca gagctaaagc atcatgacgg tcgacgctac
    13081 taagagcagg gtgatccgaa atcggattgg ggatgctgtt gccctggtgc tgcggggcgg
    13141 caaatagcgc agccgccgca ggttccggct gctccttgat atcgtcgagc aaagcaacat
    13201 cttccgggta acgcaccagg atgtcggtca agtagttgct gaactcaaaa accgtgagtg
    13261 cacgttccac cgccccaggc gagcgcaaga cggccccgta gcgctgggag ttggtggttg
    13321 ccgaactcaa aaagcggtcg agatttcgtc gcccctgcgg aggcaaatta gcgtggctga
    13381 ttaccgccag caatcgcggt gcgtccacgg ccaatcgttg catgatctgg ctgtaagaat
    13441 tctcggccga cgctgaaact tgtgcctgca gacgcaggtt tccttcggcg tcaataaact
    13501 gctgactttg ttcctggtac acgatacgtc ggtaaatttc cgccacggtc gccatccggc
    13561 tttcaatctg ccgcacgaat tcgtccggcg ccggggccgt ggacccttca cggttcaagc
    13621 atttcgccag cgccctcaat tccattggac tggatggcaa ctgatgattc tgcctcccct
    13681 ggcgtaactg cagcaggtgc tccaaatggc gcaggaattc ataagcactg gtcaggttgt
    13741 catagtcctt tccgctgatg tgttccttat cgtggagctt ctgcagcgcg aaaagcgttg
    13801 cacgcgaacg cagccaacct tcttcgccgc cgtacacccg ctgcaggcac tgcaccagga
    13861 actcgatatc gcggattcct ccccgatcaa gtttgacgtc gattgcgcgt cgccccgagc
    13921 ggcccggccg tgccttgttt ccgcgcttat cgaatcgctc cctgctctgc aacgcggtct
    13981 tgacagccgc gaagttcacg ttcgggcgat acacgtgggg cgccacatcg cgaataaact
    14041 cgcgagccag gtcggcgtcg cccgcggaat ggcgggcctt gatcatggct tgcagttccc
    14101 agtcctgagc aacctgcgag tagtattggg ccgctcgcgg cagcgctaaa gccacttcgc
    14161 cctcgtggcc ctgaggacgt aaacgcaggt cgatgcggaa cacctgtcct tcccccgtgg
    14221 ggcgtgacaa caactccgtg gtttgctggg cgagctgaat gaaatactcg cggttcgaga
    14281 tgccctgaga cggtggctcg acaccgccgt cgtaaagaaa catcaggtcg atatcggagc
    14341 tgtagttcaa ttcgttgccg ccgagtttgc ccaaggacag gactgcgaac cgcgaatccc
    14401 gcaaccgacc ttgcggatca accctcaggg gagtcccatg tctgtgctgc aatgctgaat
    14461 tcacggccag caatgcttcg tcgatcagtg cgtctgacag ggcagagatt tcctcggtcg
    14521 tctccgccag cttggcgatc ccgagcacgt cgcgcaaaag gattcgcacg tactcccgtt
    14581 tgcgaaagcg cgccagcgat actgccatgt cactgtccgg cgagcgcgag cgcatgcggg
    14641 cgaactcttc ccggaactcc tcccgtgagc aggagcgatc caggctttta ttccgtccga
    14701 atcttcgcag tagttcaatg ttctggataa gcgtctcgcc caaccaggcg ctatagccaa
    14761 atacgagcgt cgcgtaatgg atcaaaatcg gctgcttctc aaaagctgct gccatctcgc
    14821 gcggggccgc ttccatgagg cgctcgaact gcaccaccac ggaatccgga ctcggcgatg
    14881 tggtaagaaa ctggaggaac gccgcaaaaa ccgactcgga cacaacctca cgcaatcgcg
    14941 agaggttctt tgtggccgcg actggatcat caaactcgat gtcggacgga agttgtactg
    15001 cgccggattg tcgattctcg gtcaaggcgt ggccggagga cgaaagtgat cggctgtcag
    15061 gccccgaatc gcctccgaac cgcaaagata gcatgcacac tctacagcga tgtgcggatt
    15121 catttgactt gcgctttcca cgcatttacc ttcgctgttc gcaacacagt ttcctgccac
    15181 tatcgtatgc ggagcaatct ttgcactggc tgaacgcgct gttcggattc gatcgccacg
    15241 gaaccacggt cacccgcgaa gtcgtggctg gactcacgac gtttaccacc atgtcgtaca
    15301 ttgtggtggt aaatcccgcg atcttgtcgg cggctgggat tccggcagga ccatcgttcg
    15361 tttcaaccgt gctggtggcg gtcgcaggat gctacctcat ggcgttttac gccaaccgtc
    15421 cattcgcaat tgctccctac atgggagaga acgcgtttat tgcgttcacg gtttgcagga
    15481 tgctcggctt cgcctggcag actgcccttg ccgctatctt cattgccggc gtgttgttcg
    15541 tcgtgatgac ggtgttgcgc ctgcgccaat ggattgtaga gggcgtgccc acctgcttgc
    15601 gctacagctt cgcagtcgga attggattgt tcctgacgtt catcgggctg aatcagacgg
    15661 gactggtggc gcttggagtg cccggcgctc ccgtgcaggc cggacatctg acctcgcatc
    15721 cagcgctggt ggcgatgttc ggcttcgtgt tactagcggt cctcacgata cgcaagtttc
    15781 cgggcgcgat cctggtggga atcatcgtca cggcgattgt cgccttcctc gcgggtaccg
    15841 ccgctccgcc acatcacctg gtgagtctgc caccaagctt gagcccgatc ttctggcaac
    15901 tcgactttcg tggagcgctt acgtggcggg cattccccgt cgcgctgaca attttcatca
    15961 tggcgttcgt ggataccatg ggcacgttga tcggactctc cgctcgcgcg ggtttcctcg
    16021 atgagaatgg acagctgcct cagatcgagc gccccatgct agtggatgcc ttgaccactt
    16081 gcctcgctcc ggcactggga accaccacgg cgggtgcgtt tgttgaatct gcgaccggga
    16141 tcgaagccgg aggacgaaca ggtttcaccg tcttgattac tggaacctgt ttcctgctga
    16201 cattgttctt ctccccgttt gtggcggcga ttccggcaca agcttacgga ccggcgctga
    16261 ttatcgtagg cctgttcatg ctgtcgcccg taactcgtat cgacttcagc gactattccg
    16321 aatccattcc ttccttcgcg gtcgtggtac taatggcgtt caccttcaac atcgcaattg
    16381 ggatatctgc cggcttcgtg ctctatccga tctgcaagct ggtggcggga aaaacagccc
    16441 agctcaaacc cggactgtgg gtcttgacct cgctgtcgct gctgtttttc gtgttctacc
    16501 cgtatagctg agtcgccgtt tcgggcaggg atgctgcttt ttgtggacgt gctccgctaa
    16561 gagtggtggg cccggaggga ttcgaacccc caaccaaggg attatgagta cttgtggaac
    16621 gaggaatcaa caactcagct cagtttgcat ggagtgggat tcactagtat ttatgcgggt
    16681 ttctcaatca ttgagccacg gcccaccaaa cgaatacaag cggccagtgg gcacaaaatt
    16741 gggcacagtc gaagggagtg ggttgaaccc ctccgttgac ccaaccgtga aaattgaaag
    16801 aacgactatg gagtatggaa acggcagagg ccggcagaaa tcggcctgaa cagaaacgcg
    16861 ccgtgccggt gttaccagca ccgacacgac gctaagcagg gcacctgaat tggaggcacc
    16921 caatggctgc acaccaccgt aacaccgcat catcactggc tcaagaatcc caaattgcaa
    16981 ttagtcgcgt ggctctttac gcacgggttt caaccctgaa cggccaagac cccgagatgc
    17041 aactctccga actcagggaa tacgcttccc gccgcggctg gagcattacc cgggagtacg
    17101 tcgatcaggg cgtgtcgggc tcaaaggaat cgcggccaga acttaaccaa ctcatggccg
    17161 atgctcaccg gcggaaattt gatgccgtgc tggtgtggaa gatcgatcgc ttcggtcgtt
    17221 cgctgaagca cctggtcaac gccctcgcag acctttgcgc ctacggcgtt gcgtttatta
    17281 gctttcgcga caacctcgat ctcagcacgc cctccggtcg gctgatgttt caaatcattg
    17341 gtgccatggc ggagttcgag cgatcgctca tccaagagcg cgttcgggct ggactgcgca
    17401 atgctcgcgc caaggggaag aaattcggac ggccccgcgc ccaggtcgac gctgaccgtg
    17461 tggccgcact gcgccaggag ggactctcgt ggtctcaagt atgtcgagcg ctaaaggtga
    17521 gcaaagggag cgcccagcga tcggttgcac gcttgcctaa cagagacaaa accgggctct
    17581 tagcgagttg cagttgagtt tcgccgccat ctcggatata cgaaggctag tgaccaaggg
    17641 aattctttgt gtgaagcttc ttgagcgatc cttcgtagtc gttctggcgc tcctcgccgc
    17701 cttagctgta gcggacgcag cacagtcggg accagccgac aatgccgcgt tgcccgaccc
    17761 aggcccgatt tatggcgcgc gtctggaagg tttcgactat ccctggccgg ttgcgctgta
    17821 ccgtttccag tcccaaggcc aggcactcga gatggcttac atggacgtga agccggcgcg
    17881 ccccaccgga cgggtcgcgg tactgctgca cgggaagaat ttctgcgctg ccacctggca
    17941 gaccaccatc accgcgctcg ttgaggccgg ctatcgcgtc atcgcgccgg atcagatcgg
    18001 cttctgcaag tcgagcaagc cagcgcagta tcagttcagc ttccagcaac tcgccggcaa
    18061 tacccatgcg ctgctcgaat cactcggtgt cgagcgcgcg actgtgatcg gccattccac
    18121 cggcgggatg ctcggaatac gttacgcgct gatgtaccca tcgcaggtcg atcaactggt
    18181 tctggtggac cctatcggcc ttgaggactg gaaggcgcgc ggcgtaccct ggcaaagcgt
    18241 ggatgcctgg tatcagaggg agttgcagac aaccgcagac cgcatccgcg cttacgagcg
    18301 tgctacgtat tatgcgggca cctgggaaac caattatgac cgctgggtgc aaatgctggc
    18361 tggtatgtat cggggcgcag gacgcgccca agttgcatgg gactcggccc tcctgtacga
    18421 catgatctac acgcagccgg tgctctacga ctttggggcg ctgcgcatgc ccgtgctgct
    18481 gatgatcggc gacaaggaca ccaccgctat tggcaaggat ctggcttccc cggaagtacg
    18541 cgccaagctc ggcaattatc cggtgctcgg caaggcagcg gccgcccgga ttccacacgc
    18601 gaaattagtc gagttccccg atctgggtca cgccccgcag atccaggcgc cggagcgttt
    18661 ccacaaggcg cggctggagg gactgcaagc gcccagcacc taccagtagg gtccgaggta
    18721 ctaatccgac acttcccaca tgccggtatc tcgggagttt gcacgcgagt ggcagttcaa
    18781 cggtttgccg acttgtgggg actaatctta ccgcggcact tcgacgacat ggcgactata
    18841 cgtccatctc ttacaatgtt gccccggtca agcacttttt gaggcgcact actccgtccg
    18901 ctcttaagag cgcgccattt cgctctcatt gaaaaatcac tggccatcat caaccaaagg
    18961 ctcgcgtgta atctctcagg tggaactcag tccgacccaa ccatctctcc ggtcgcaacg
    19021 tcgatcatga ttactacttc gaactccctc cgtagggagc gggatgccca atcaagtggg
    19081 cggtgattta cttcacacca tggacgtagt cggactgtcc cgcgtgcgaa ccgaacttta
    19141 taacctgttc gtaattccat tgcttgcgcc acatccagaa caaact
//
LOCUS       DASRKK010000540         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_632128, whole genome shotgun sequence.
ACCESSION   DASRKK010000540 DASRKK010000000
VERSION     DASRKK010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632128"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFC15_12420"
     CDS             complement(<1..328)
                     /locus_tag="VFC15_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZM11003.1"
                     /translation="MDAKPWLVLIVFLAICLGAGGLGSFFTANGVRDWYPRLRKPAGT
                     PPNWVFGPVWTTLYVLMAVSAWLVWREYGWGARSALLIFFAQLALNVAWSGIFFGSRL
                     IGVALLA"
     gene            1070..1342
                     /locus_tag="VFC15_12425"
     CDS             1070..1342
                     /locus_tag="VFC15_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM11004.1"
                     /translation="MDIVAVKTKFQIVIPQHIREQVKVEIGDLLEASVEDGKITFTPK
                     SLIDRHLAEGLEDAKHGRTHGPYANAKDAIKALETRAKRHAKKRGQ"
     gene            1339..1584
                     /locus_tag="VFC15_12430"
     CDS             1339..1584
                     /locus_tag="VFC15_12430"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM11005.1"
                     /translation="MKLRFTERADKDYAGLPVAIRKAFAKQLRFLLVNLGHPSLHAKK
                     YSQGMDVWQGRVTRGWRFYFKIERDEYVILSIIPHPK"
     gene            1722..1946
                     /locus_tag="VFC15_12435"
     CDS             1722..1946
                     /locus_tag="VFC15_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZM11006.1"
                     /translation="MQDRHDHERSRPKQVEAADLFRVRQPDVSKMLRGEFRQFSVERL
                     LRFLVALNQDVEIVVKPHGNRENASALQVF"
BASE COUNT          551 a          612 c          641 g          399 t
ORIGIN      
        1 ttgcgagaag cgccactcca atcagccgag acccaaagaa aatcccagac cacgcaacgt
       61 tgagcgcgag ctgggcaaag aagatcagca gcgccgagcg cgcgccccat ccgtactcac
      121 gccacaccag ccaggccgaa actgccatca ggacgtagag tgtcgtccac accggcccga
      181 aaacccaatt gggcggggtg cccgccggtt tacgcagtcg gggataccaa tcgcgcaccc
      241 cgttggcggt gaagaaagag cccagaccac ccgcgcccaa acagatggca agaaatacga
      301 tcagtaccaa ccacggtttc gcatccacgg aagtattgta tagagctatc agccgtgagc
      361 tatcggccgt cagccaaact gcggtttgtg cgggtagagc agtgcggacc ggggccccca
      421 atccgcgccg cttttgcgtg gattggggtg gttgagcgcc gcgttgcagc gaatcctaaa
      481 gaaacgagcc ggcttcagca ctgctccata ccggctacac aatgcctaaa ccgcaaagga
      541 gctcagcgca tcggcttaag aagacgcgag cccgtttccc aaggggcacc gatttaggga
      601 gttcgctcta gatctggccg gtctcggcac cgttaacgct cctcccggta gcgcagcttc
      661 attattagca aaggcgcgct gaggaagaaa gtgacagcat tcggcaagtg actgtgagga
      721 tgagtttctc ctactcattc gcaaaaggcg cggatgagtg ggcccctggc aggcgtgtct
      781 gcgcgcggta ttcctcccct tgacgcaata tagtgaaatc actgtaatga tcgccaatga
      841 ccgatgaagc gtctttgaag cgcgagtttg cctaacgaat tggatctgag cgcctgagca
      901 ggcgtgagat cccgtctccc acccaaagcg ttcggaacgg cggaaagcgc agggaacgag
      961 tcactttaac ggtgccgttt cacttccaaa ttcatggccg actcgagggg attgacaggg
     1021 gctatcccag gttgtactgt tcaacagttg aactggaaga cctaccatca tggatatcgt
     1081 cgccgtaaaa acgaagtttc agatcgtgat tccgcagcac atccgcgagc aagtcaaggt
     1141 ggagatcggc gaccttctgg aagcgagcgt ggaggacggg aaaatcacct ttacgccgaa
     1201 gagccttatc gaccggcatt tggcagaagg tctcgaggac gcaaaacacg gcaggaccca
     1261 cggcccttac gccaacgcca aggatgcgat taaggcgctg gagacgcgcg ccaaacgcca
     1321 tgcgaaaaag cgcggccaat gaagctccgg tttacggaaa gagcggacaa ggactatgcg
     1381 ggcctgccgg tcgctatccg caaagcgttc gcgaagcaac tgcgcttcct gcttgtcaat
     1441 ctcggacatc cgtcactaca cgcgaaaaaa tacagccagg gcatggatgt gtggcagggc
     1501 agggtaacga gaggatggcg gttctacttc aaaatcgagc gggacgaata tgtcattctc
     1561 tccatcattc cgcatcctaa gtaatcggca tacccgcccg atccgtttcc aggagatgaa
     1621 gccatgagca acaggaaggg acatgaaacc gggaaccgca atgtgttcaa ggacatcggc
     1681 gtgccgaatg cggaagagca tttggtcaag gcgcagcttg tattcaagat cgccacgatc
     1741 atgaaagatc gcggcctaaa caggtcgagg ccgccgattt gttccgagtc cgccagcccg
     1801 atgtatcgaa gatgctgcgc ggcgagttcc ggcagttttc cgttgagcgc cttctgcgct
     1861 tccttgtggc gctcaatcag gatgttgaga tcgtggtaaa gccgcacggt aacagggaaa
     1921 acgcttccgc cctgcaagtc ttctgaaaga cgccggtgcg gatggtaccc gacactgccg
     1981 aaacgactat gggttatagc aatggtggaa atgctctaaa gctggccggc cttggcaccc
     2041 ttaccggtag cgcagcttca tcatcagcag aggcgcgctg aggaagaaag tgaccgcatt
     2101 cggcaagatg atgggcagcg agtggatcat gaagccgtag cctacccaca gcaagagccc
     2161 cacgttgagg ctaatcaaca tgagcagcga gagatcgcgg acg
//
LOCUS       DASRKK010000541         8602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1415229, whole genome shotgun sequence.
ACCESSION   DASRKK010000541 DASRKK010000000
VERSION     DASRKK010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415229"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..792
                     /locus_tag="VFC15_12440"
     CDS             151..792
                     /locus_tag="VFC15_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZM11007.1"
                     /translation="MVTEHRLGDIIANSVTHGVGAALALAGAVVLVVTAVGGTAWQIA
                     SCSVFGCTLVLVYVSSTLYHSLVRTGARHVLRIIDHSAIYLLIAGTYTPFALVSLRGQ
                     LGWLLFAIVWNLAILGIIFKSFAIERFAIASIVVYLGMGWLAVFAMRPLQHALTWHGI
                     FWIALGGLFYTVGIVFYALDRISYFHALWHLFVLAGSTSHYFAVLFYVAQPRT"
     gene            complement(890..1237)
                     /locus_tag="VFC15_12445"
     CDS             complement(890..1237)
                     /locus_tag="VFC15_12445"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZM11008.1"
                     /translation="MKFQQFSFGQIRIDGIKYGYDIVIDRGEIRERKKKPSKRFRDSF
                     GHTPLSLEEAIPWKCSRLVVGTGTGALPVMDEVRREARRRKVELIILPTEEAMELLNR
                     RAENTNAILHVTC"
     gene            1339..2130
                     /locus_tag="VFC15_12450"
     CDS             1339..2130
                     /locus_tag="VFC15_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZM11009.1"
                     /translation="MSSSSFGKHASGATSSQTAVPEPSFAERARTLMHSGRIGSLSTL
                     SRKQPGFPFVSLMPYALGDQGRPVLLISTMAMHTQNLQADPRASLFVTEPGASGDPLG
                     ASRVTLIGTVSRIPEPELAAVRTVYLTRFPDSKYWVDFDDFFFYRMDVLDVYYVGGFG
                     VMGWVAASEYSQAQSDPLAEHNSDIMQHMNADRKDALILLAKRFAGIEAPEAEMTSVD
                     RLGFHVRLKTQDGMRGARIGFLREVTNPTQTREVLVEMVKLARQE"
     gene            complement(2257..3081)
                     /locus_tag="VFC15_12455"
     CDS             complement(2257..3081)
                     /locus_tag="VFC15_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZM11010.1"
                     /translation="MSGRITILLALAAVSANYVYSWVAALRHDRTAGLVNGQAQAPIP
                     SLLQLATGSLTNFFDTLGVSSFATTTTIFKLGRMVPDELIPGTMNVGHTLPTIAQAFI
                     FITLVQVDFTTLVLLIASSVIGAWWGAGVVSRLPRRAIQLGVGGALTLAVVFMIMREL
                     HWFPAGGEGLSLHEFGLLTGMAGTCLFGALSTLGIGFYAPCMILVSLLGMAPLLAFPI
                     MMGSSAFLMPVGSLRFLKSRRYEARAALGLALGGLPAVLIAAFIVKSLPLDTHAVW"
     gene            complement(3143..4249)
                     /locus_tag="VFC15_12460"
     CDS             complement(3143..4249)
                     /locus_tag="VFC15_12460"
                     /EC_number="4.2.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF043002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cis-3-hydroxy-L-proline dehydratase"
                     /protein_id="HZM11011.1"
                     /translation="MPKITKIEAFQVDLPLREGSYKWSGGNFVEVFDSTVVAVHTDAG
                     ITGYGEVCPLGPAYLPAYAKGARTGIAELAPHLIGFDATALSLLNDRMDRALRGHPYV
                     KSAIDIACWDILGKVCGQPVVTLLGGRYGESFPLYRAISQESPEEMAGKVLRYRSEGY
                     TKFQLKVGGDPDTDIQRIHEVAGNLKTGDVLIADANTGWTQHQAIRVADAVRSVDVYL
                     EQPCASYQECLTVRRHSNRPFVLDEVVDCLAMVVRGFTDQAMDVINLKISKVGGLTRA
                     REIRDLCVSLGIALTIEDTWGGDITTAAIAHLAHSTPPAYLFTSTDFNSYLTVSNAEG
                     APQRKDGRLAAGCAPGLGIEPRWNVLGKPVLSIS"
     gene            complement(4696..5121)
                     /locus_tag="VFC15_12465"
     CDS             complement(4696..5121)
                     /locus_tag="VFC15_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZM11012.1"
                     /translation="MKEIHSTRSSDEVNKSPGKSECLVLLAHGSKDPRWRVPFERIVD
                     AVQGQSGETKVSLAYMEFLEPTLIDVARECVGQQILHLKILPLFFSIGAHLAIDVPEQ
                     VNQAKAQFPQLEVEVLSPIGEDPRLTGLIQQIVMETLKH"
     gene            complement(5502..6905)
                     /locus_tag="VFC15_12470"
     CDS             complement(5502..6905)
                     /locus_tag="VFC15_12470"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZM11013.1"
                     /translation="MCQTAFTKTLAQIASDPRVTVRRAGPPDPAGRCVVYWMQRAQRV
                     LDNSALDTAVRVANFLQQPVVIFFAPVPFYPHANLRHFVFLAEGIPQTAAGARARGIG
                     YVLRRYPEHSLLKFCEEVKASLVVGDENPMREPKCWRELAARKLTVPLWTVDADVIVP
                     SKLLAKEQYAARVIRPRLQARLQEFLIPHPNPKARVEWEKPRRLSVLPDDGSADITEG
                     WKELDRSVQPVTSFHGGTDRALTLLREFVTGKLAHYPERHGKPELDGTSRLSPYLHFG
                     HIGPQTVSLAVEQAKVPRQVKDDFLDQLITWRELSINFVHFNPLYDSIESAPDWAHRT
                     LAAHAGDPRPRSYSRQQLERAETYDDLWNAAQRQMLHAGWMHNYMRMYWAKKILEWSP
                     SPAVAYQTAVYLNDKYFLDGRDPNGYAGIAWSIAGKFDRPWFDRPIFGTVRYMSGNAA
                     RKKFDAERYIEQMAKLA"
     gene            7344..>8602
                     /locus_tag="VFC15_12475"
     CDS             7344..>8602
                     /locus_tag="VFC15_12475"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HZM11014.1"
                     /translation="MSGSERVAIAGVTGYIGGRLAPRLLDHGYAIRCLVRSPAKLQSR
                     GWATNPDVQIRQTNLGDETCLTCDLEGCGAAFYLVHSMMSAGSDYAEHDRQLALTFAR
                     AAKNAGVKRIIYLGGLGETGPDLSEHLSSRREVEEALASTGVAVTVLRAAMIIGSGSA
                     SFEILRYLVERLPVMVTPKWVSTPCQPIAVRNVIGYLVGVLAVPETAGGVFDIGGPDI
                     FCYRDIMRIMAEELGLRRRLVIPVPVLTPRLSSYWIQLITPLSNTIASPLAEGLKNPV
                     VCRDNRIASLIPQELLAVRAAIQGALSQVAEHHVETSWSMAGPIPGDPDWAGGTVFRD
                     VRQTTLDIPDWAVFRAVCRVGGGNGWYATDILWKIRGWIDQLVGGPGLRRGRRDSEVV
                     GYGEALDFWRVVGFERDRRLALRAEMKL"
BASE COUNT         1721 a         2483 c         2531 g         1867 t
ORIGIN      
        1 gagtgtgctt ctgctccaac ggtcgcgccc ccagcctctg tgaccggatt taacgtggtc
       61 tgccccaatt gtagccgcga gttttcctat ttaccgaacc gatgttgcag cgccgtgtct
      121 actgcacacc ctgatgttta tcatcttcct atggtcacgg aacacaggct gggcgacatc
      181 atcgcgaact ccgtcacgca tggcgtcggg gcggcgctcg ctctcgcagg ggctgttgta
      241 ctggtggtca ctgcggtggg tggaaccgcc tggcagattg ccagttgctc ggtctttggc
      301 tgcacgctgg tgctggtcta tgtcagctct actctgtatc actcgctcgt ccgcaccggc
      361 gcacgtcacg ttctgcgaat cattgatcat tcggcaatct atctgctgat cgccggaacc
      421 tacactccct tcgcgctggt gtcgctgcgc gggcagttgg gatggcttct tttcgccatc
      481 gtatggaacc tcgccatcct gggaatcatc ttcaagagtt ttgcaataga acgattcgcc
      541 atcgcgtcga tcgtcgtgta tctgggaatg ggttggcttg ccgtgttcgc gatgcgcccg
      601 ttacagcacg ctcttacgtg gcacggcata ttctggattg cgctgggtgg cctgttctat
      661 accgtgggca tagtcttcta cgctctcgac cgcatttctt acttccatgc gctctggcat
      721 ctctttgtgc tggccggcag cacctctcac tacttcgccg tgctgttcta tgttgcccag
      781 ccgcgcacct gacgcgcaaa ggcctcgtca ctgctcgctg tcagctttcc tcggatgaag
      841 tcttcgacgt ctaaccgcat gccggcgccg gtctggcgaa ttctaacgct cagcaggtca
      901 catggagaat ggcattggtg ttttcggcgc gccgatttaa gagctccatg gcttcttccg
      961 ttgggaggat gatcaattca actttgcgcc gccgagcctc acgtctgacc tcatccatga
     1021 ctggcagtgc tccagtgccc gtgcccacca ccaaccgact gcacttccac ggtatcgcct
     1081 cttccaaaga gagcggcgtg tgaccgaaac tgtcccggaa ccgctttgac ggtttcttct
     1141 tgcgctcgcg aatctctcct cggtcgatca cgatgtcata gccgtattta atgccatcga
     1201 tgcgaatttg accaaatgag aactgctgga acttcatagc gggcctctga taaggataca
     1261 accgaggcgc tgcgtggcct gccagcactg cgtgatggca agttgagggt tatgctgata
     1321 ccgagagggt gttaagcaat gtcttcctca tccttcggaa aacacgcgag tggcgcgacg
     1381 agtagtcaaa ctgcagtccc cgagccttcg ttcgccgaga gagcacgcac gttgatgcac
     1441 tcgggccgca ttggtagtct ttcgaccctg tcgcgaaaac aacccggctt cccctttgta
     1501 tcattgatgc cttatgcgct gggcgatcaa ggccgtcctg ttttgcttat cagcacgatg
     1561 gccatgcaca cgcagaatct gcaagcggac ccgcgcgcca gcctgtttgt gaccgagccc
     1621 ggcgccagcg gcgacccgct cggtgcgtcc agggtcacgt taataggaac ggttagcaga
     1681 ataccggagc cagagctggc ggccgtgcgg acggtctacc tgacgcgttt cccagacagc
     1741 aagtactggg tcgattttga cgatttcttc ttctaccgaa tggatgtatt ggacgtttat
     1801 tacgtgggag gcttcggagt gatgggatgg gtggctgctt cggagtactc ccaagcgcaa
     1861 tccgatccgc tggctgaaca caatagcgac atcatgcagc acatgaatgc cgaccgcaag
     1921 gatgcgctca tcctgctcgc caagcggttt gccggcatcg aggcaccaga ggcggagatg
     1981 acctcggttg atcgcctcgg ctttcacgtc cgcctgaaga cccaggatgg catgcgcgga
     2041 gcgcgcatcg gcttcctgag agaagttacg aatccgacgc agaccagaga agtgttggtc
     2101 gagatggtga agctggcgcg tcaagagtaa atcatctcgt cgctgtgtca ttttgtgccg
     2161 gtttaagcgc caaggtaaca gcgtagactg ccgctcaatg cccctgacgg taggcagagc
     2221 gaaagagcag gaaggccgcc cacagtgcga tggccatcac cagacggcgt gggtgtcgag
     2281 tggtagggac ttcacaataa atgcggcgat cagcaccgct ggcaagccgc caagcgcaag
     2341 acctaaagcg gcgcgggctt cgtagcgcct gcttttcaaa aatcgcagac tgcccaccgg
     2401 cattaagaaa gccgaggagc ccatcatgat gggaaaggcc agaagcgggg ccatgcctag
     2461 taggctgacc agaatcatgc aaggcgcata aaagccgatc cccagggtgc tcaaggctcc
     2521 gaataggcac gttcccgcca tcccagtgag aagaccgaat tcatgcaagg acagtccctc
     2581 tccgcccgcc ggaaaccagt ggagttctct catgatcatg aagacgaccg ccaaagtgag
     2641 cgccccgcca acgcccaact ggatggctcg cctggggagc cttgagacca cgccggcgcc
     2701 ccaccaggca ccaatcaccg atgaagcgat gagaagcacc agggtggtga aatcgacttg
     2761 aaccaaggtg atgaagataa acgcctgggc gatggttggg agcgtatgcc ctacgttcat
     2821 ggtgccggga atcagttcgt cggggaccat ccggcccaat ttgaagatgg tggtggtggt
     2881 cgcgaacgaa ctcacgccga gggtgtcaaa gaagttggtc agagagccgg tcgccagttg
     2941 gagaagcgat gggatgggag cttgtgcctg gccgttcacc aagcctgccg tgcgatcgtg
     3001 gcggagcgcc gctacccagg aataaacgta attggcagac accgccgcaa gagccaggag
     3061 gatggtgatg cggccggaca cggttaacgc tgctctcttt cccttaccgc taggatcttc
     3121 cggaccaggt acggcctccg cttcaagata tggacagcac aggcttgccc aacacattcc
     3181 atcgcggctc gatccccaac ccgggagcgc atccggcggc gagccggcca tctttccgtt
     3241 gcggcgctcc ttcggcattg ctcacggtca ggtaactgtt gaagtcggtg gacgtgaaca
     3301 agtaggccgg cggagtactg tgcgccagat gagcaatcgc cgccgtggtg atgtctccgc
     3361 cccaggtgtc ctcgatcgtc aaagcgatcc ctagcgaaac gcacaagtcg cggatctccc
     3421 gtgcccgggt aagtccgcca actttggaaa tcttcagatt gatgacgtcc atggcctggt
     3481 ctgtgaaacc ccggactacc atcgccagac agtccacgac ctcgtccagc acaaagggcc
     3541 ggttcgaatg gcggcgcacc gtcaggcatt cctggtagct ggcgcagggt tgctcaagat
     3601 agacgtccac ggaacgcacg gcatcggcaa ctcggatcgc ctggtgctgg gtccaaccgg
     3661 tgttggcatc tgcgatcagt acatctcctg ttttcagatt gccggccact tcgtggatgc
     3721 gttggatatc ggtgtccgga tcgccgccta ccttgagttg gaattttgtg tagccctccg
     3781 aacggtaccg taagactttt cccgccatct cctcggggct ttcctgggag atggcccggt
     3841 acagcgggaa tgactcccca taacgtccgc ccagcagagt caccactggt tgaccacaaa
     3901 ctttcccgag gatgtcccag caagcgatgt cgatggcaga tttcacgtaa ggatgtcctc
     3961 gcagggcacg gtccatgcga tcgttgagca gggagagcgc tgttgcatca aagccgatga
     4021 ggtggggggc aagttccgcg atgccggtac gcgcgccctt ggcataggct ggcaagtaag
     4081 ccgggcccag gggacagact tccccgtaac cggtaatgcc ggcgtcggtg tggacggcca
     4141 cgaccgtgga gtcaaagacc tccacaaaat ttccgcctga ccacttgtag ctgccttccc
     4201 gcaaaggcag gtctacctgg aaagcctcaa tttttgtgat tttcggcatt accatatctc
     4261 ttttgcacct acctcggcct tcgcgtggtg ttctctcccg ggaaatcccc tgagcgaggg
     4321 ctcagcgctg acaccgcgat tcttgcctgg tgggctgcgg cggcaagaat cgaaacgaaa
     4381 acagttgctt tcctgaaact gcttcgtgct cgcaatcata gcctctaccg tcctcgcggc
     4441 caagctccgt tgccgagcac cgtgataatt gcttgatcct tggtgatggg tttggcctct
     4501 aaccaatatg tgcgaatttg cttttggatg agtttgaggt ccagcagatg ctcgcggatc
     4561 tggtcacctt atggattgtc tgtccgaggg taggtggtcg gctttggttt tgggattttg
     4621 gttttaggct ggcaagaaag gcaacgtggc aactgatcat ttcgtcgggg ccctgctcgc
     4681 ttcgatcaca actggtcaat gctttagggt ttccatgaca atctgctgaa tcaatccagt
     4741 caatcggggg tcctcaccga tgggcgacag cacctcgacc tcaagctgtg gaaactgcgc
     4801 tttcgcctga ttcacttgtt cgggtacgtc aatcgccagg tgtgccccaa tcgaaaagaa
     4861 caagggaaga atcttcagat gcaggatttg ttgtccgacg cattcgcgcg ccacatctat
     4921 cagggtcggc tcaaggaatt ccatataagc caagctcact ttagtttctc ctgattgacc
     4981 ctgcacagcg tcgacaatac gctcgaacgg aacacgccac cgcgggtctt tactcccgtg
     5041 cgccagcaac accaggcact cactctttcc aggagacttg tttacttcgt cgctggaacg
     5101 cgttgaatga atctctttca cgggccctta tccgcgctta caggcgctgc gctcaatcac
     5161 aatgccccga accgctgtcg cataaccccg gaacgatacc gaaagacttg ccgtgacgtc
     5221 ggaacgatga acaagcggtt agcaaggcgt cccagctcga ctccatccaa ccgcagcccg
     5281 ctgccggccg ccttctaatg cgattcccag gaaagtgtat cgcgcgccat gtaactgcca
     5341 agcttgtatt cttttgtata tgactggcaa gcggcgcgtc gctctcgcag gcgattacga
     5401 tcccgctgtc atcgcgcacc agacttttca cgggcgatgc gatcggtttc cgccggcagc
     5461 tgcgggacgg acggtcattc tgattggtaa atcgggcgat ctcacgccag cttcgccatc
     5521 tgttcgatgt agcgctcggc atcaaatttc ttgcgcgcgg cattgcctga catgtaacgg
     5581 acggtgccaa aaattggccg gtcgaaccag ggacggtcaa acttgccggc gatggaccac
     5641 gcaattcctg cgtagccgtt cggatcacgt ccatcgagga aatatttgtc gttgagatac
     5701 accgctgtct ggtacgccac cgcgggcgaa gggctccact ccagtatctt cttcgcccaa
     5761 tacattcgca tgtagttgtg catccacccg gcgtggagca tctgtcgctg cgcggcattc
     5821 cagagatcgt cgtaggtctc ggcgcgctcc aactgctggc gcgagtagct ccggggccgg
     5881 ggatcgccgg cgtgcgcagc gagggtgcga tgggcccagt cgggtgcgct ttcgatggag
     5941 tcatacaacg gattgaagtg cacaaagttg atggacagct ctcgccaggt gatgagctga
     6001 tcgagaaagt cgtccttgac ctggcgtggc actttggcct gctccaccgc cagcgagacg
     6061 gtttgtggcc cgatgtgtcc gaagtgcagg tacggcgaca atcggctggt accatcgagc
     6121 tccggcttgc cgtggcgctc gggatagtgg gccagcttgc cggtcacaaa ttcgcgcagc
     6181 aatgtcaaag ctcggtcggt cccgccgtga aaggacgtga caggctggac tgagcgatca
     6241 agctccttcc aaccctcggt gatgtcagcg gatccgtcgt ctgggaggac agacagccgg
     6301 cggggcttct cccactcgac cctggccttg gggttcggat gcgggatcag aaactcctgc
     6361 aaccgcgctt gcagccgcgg acgaattaca cgagcggcgt actgttcttt cgccagtagc
     6421 ttcgaaggta caatgacgtc ggcatccacc gtccacaacg ggacggtcaa ctttcgggcc
     6481 gccagctcgc gccagcactt cggttctcgc atagggtttt cgtcgccgac caccagcgag
     6541 gccttcactt cctcgcagaa tttcagcagg gaatgttccg gataccggcg cagaacatat
     6601 ccgataccgc gcgcgcgagc gccagccgca gtttggggaa tgccttccgc cagaaacaca
     6661 aagtgccgca ggttggcatg gggatagaac gggactgggg cgaagaagat aaccactggc
     6721 tgctgcagga agttcgcgac ccgaaccgcg gtgtcaagtg ccgagttgtc gagtactcgc
     6781 tgcgcgcgct gcatccagta cacgacgcag cgtcccgctg gatcgggagg tccagcgcgc
     6841 cggacggtca cgcgcgggtc ggaagcaatc tgcgccaggg ttttcgtaaa ggcagtttgg
     6901 cacatcgtga atgaatccgc caggagtatg gtgtggccaa gttggcgccg caccacctgc
     6961 gtcggacctt tgccaggtcg tgccaagcat cgggacacga gttggagcag atacaattcc
     7021 ttttggggca cgtctgggtt caaacgaccg aacggtatct tggctgcaag cagcgggcac
     7081 aaatagttca gtaatttaag cgctaagctg ggatttctgc ccgcccgccg ccaatgacgt
     7141 agtcttttcg ccgacttcaa gtggtttctc ctcgccaacg ctgggcaatc cgtttgggct
     7201 ttttcagcgc ggacggtgca aaattcgcca tcgccaagtc tttcgtaaag ttgcgctcat
     7261 ctatgtactt ggccgcagat gccgggtgtc aaccaggtaa tgctgtggag tcgaactgag
     7321 aacattggct gccaaggaga cttgtgtctg gatccgaacg cgtagcgata gccggggtca
     7381 ctgggtacat tggtggccgc cttgcccctc gtctgctcga ccacggctac gctattcggt
     7441 gtctggtgcg ttcgccagcc aaactgcaaa gtcgcggatg ggctacgaac cccgacgtcc
     7501 aaatcaggca aacgaacctg ggagacgaaa cgtgcctaac ctgcgacctg gaaggttgtg
     7561 gggccgcgtt ttacctggtc cattcaatga tgtccgcggg atcggattac gccgagcacg
     7621 atcgtcaact ggctttgaca ttcgcacgtg ccgccaagaa tgccggagtc aaacgaatca
     7681 tctatcttgg tggactgggc gagaccggcc cggacttgag tgagcatctc tcctcgcggc
     7741 gagaagtcga agaagcgctt gcctcaactg gcgtagctgt tacggtgctg cgcgccgcca
     7801 tgattatcgg atcaggctcg gcatccttcg aaatcctgcg ctacctggtg gagcgattgc
     7861 cagttatggt cacgcccaaa tgggtgagta ctccgtgcca gccaattgcg gtcagaaatg
     7921 tcatcggata cctggtcggc gtcctcgcgg taccggagac tgccggcggc gtattcgaca
     7981 ttggcggccc agacatattt tgttaccggg acatcatgcg cattatggcg gaggagctgg
     8041 gactgcgccg ccgtttggtt attcctgtac ccgtgttgac gccacgcctg agctcttact
     8101 ggattcagct gatcacgccc ctgagcaaca cgattgccag tccactggca gagggactca
     8161 agaatccagt cgtatgtcgg gataaccgta tcgccagtct catacctcaa gaattacttg
     8221 ccgtccgcgc cgcgatacaa ggagctctca gccaagttgc tgagcaccac gtggagacca
     8281 gttggtctat ggcaggtccc attccgggcg acccggattg ggcaggcgga accgtttttc
     8341 gtgacgtccg ccagacgacc ttggacatac ctgactgggc ggtctttcgc gcggtgtgtc
     8401 gggttggcgg tggaaacggg tggtacgcga cagacattct ctggaagatc cgcggatgga
     8461 tagatcaatt ggttggcggc ccggggctgc gacgagggcg aagggattca gaagtcgtcg
     8521 gatacggaga ggcgctggac ttctggcgcg tggtgggttt cgagcgcgac cgccgcctgg
     8581 ccttgcgcgc cgagatgaag ct
//
LOCUS       DASRKK010000542        13686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1316763, whole genome shotgun sequence.
ACCESSION   DASRKK010000542 DASRKK010000000
VERSION     DASRKK010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13686
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316763"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="VFC15_12480"
     CDS             complement(<1..1890)
                     /locus_tag="VFC15_12480"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HZM11015.1"
                     /translation="MASKLHTKNEFENPVAHASIRIQPPSPMARDLQSRFLALLLALF
                     SVAAIVFASINFQKDREYFTPYDGVWWVEDGDHLQAQRVDANGPGEKAGVKKGDWLVA
                     VDGRNLSNVASLEQQLYRTGIWSKATYSLVRQGIPVEAPLILAPADRSLYLGLRLIAL
                     VYLGIGLYVLLRRWTAPKSAHFYVFCLVSFVFYSFHYTGKLNDFDWIIYWSNVVAWLL
                     QPALFLHFSLTFPERKALLTGRRWLVPLVYLPGALILTLQIVAVRWLQPSETLRFNLD
                     RLAMVYLAAYFLTATFVLWHSYRHAGSAILRQQMKWVTRGTILAVAPFTVFYVIPYLL
                     GSVPTAGMRFSVLSLVFLPLTFGYAIFRYRLMDVDLIFKRGMAYTIAGGAILAAYFAA
                     VGVVSELFHKNFPSAGPVGLVIAIAVTALLFDPFKNWVQDYLDKLFYKKRYDYRKTLI
                     EFGRDLNSETDLDSMLTSIVDRLSRTLMVDRMAVFLSNPESPEQFALAKTFGIANTGG
                     LDLDFLHVERPEWAAGHLFFDNTSQAVRETPQAREAIRRLELNYYIPCTVMNRMIAVL
                     GLGKTAEGDFLSSEDVQLLETLAGYIGIALQNARLYKSLESKAAQYERLKEFNENIVE
                     SISVGV"
     gene            2142..2987
                     /locus_tag="VFC15_12485"
     CDS             2142..2987
                     /locus_tag="VFC15_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM11016.1"
                     /translation="MNFASVGASGLSLAAATSWGGGDFCGGLAAKRASVFRVVAVAHG
                     CGLIAMLTMACLTGEAIPPRADLEWGAFAGLVGAFGIAALYKALAIGRMGVVAPVAAV
                     ITGVLPVLVGFRTEGMPDLAQLFGFALALVSIWLVARPNEDVDSHRGLGLAILAGVMF
                     GLFLVAGKQAGHHGVFWPLVAARFISTLLMLALVAISPRDPQPLRTVFWPMILSGLLD
                     SAGNALFIAATRHGRLDVAAVLSSLYPASTVILARVFLKERISSWQGVGIVGALASVA
                     LISWR"
     gene            complement(3641..4216)
                     /locus_tag="VFC15_12490"
     CDS             complement(3641..4216)
                     /locus_tag="VFC15_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11017.1"
                     /translation="MKRLALAIACLALLTVAASGAWANPIPTQITLGPSSAGTVTASP
                     TSEIFSGVWGWAYQGANSGNPGFTLANATIAVVGGGGGIYTLAANAENFTVNIGVNSL
                     SGTLSLNTVALPTPGLAVFVGTLSITSATAGFQSTGYPKGAVVNADFVTYQGNLSSGQ
                     LTPSVPEPSSLALLGTGVLGLAGLIRNKMKI"
     gene            4704..5957
                     /locus_tag="VFC15_12495"
     CDS             4704..5957
                     /locus_tag="VFC15_12495"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZM11018.1"
                     /translation="MPSYQEIVELEQKYLLNTYARYPVAFARGKGVYLFDIDGKRYLD
                     FLSGLGVNALGHAHPRIVKVIRDQAAKVIHLSNLYYNEYQGLLAEKLCQKSGLDRAFF
                     TNSGTEAIEGVLKLVRAAGHATAGPEKMIVVALDNSFHGRTMGALALTGQPKYRQGFD
                     PMLEGVRFVPRNDIEALRAAVNDKVCGIVLEPIQGEGGIQVSSPDFLHACREVADQNN
                     AALIFDEIQCGMGRTGQLFAFQHFGVIPDVVCVAKPMAAGFPMGAFLANDRFAKHITP
                     GKHGTTFGGGPLACRIALEYLAILEEENLLYQVRRVGAYFTTKLEELVAKFDIATEAR
                     GVGAIQALQLNIPGKPVLDGALADGLLINVTQDSVLRFLPPFLLQEKHVDAGMRILKK
                     QLASAQKQHKAEMKSAKQKSTAATA"
     gene            5966..8410
                     /locus_tag="VFC15_12500"
     CDS             5966..8410
                     /locus_tag="VFC15_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM11019.1"
                     /translation="MSAFLQDLAFGFRLMRKSPLFYLVAVLLLALGIGANTAVFSVVD
                     AVLLRKLPFRDPDALAMVWEKNPQLGALVGDRVPTSYTNFEEWVRQAKSFDGIAGFED
                     VSLNRTGNGEPERVAGARVSPNFFQVLGVNPAAGTTFDFVERDPSQSHAAMLSYAYWQ
                     SHFGGSRSIVGQTITLNDESYSVVGVLPANFYLPSTRQGSEQRKPDIWIPYDASRKGT
                     EAERNRRRMQVFARLRPGVTLQQSRDEMQTVARRLEEQNTALNAGFSANVFPVYVEDL
                     GQELRRNLLVLLSAVGMVLLLACANLANLMLTRATARQRELAIRKALGASRARLIRQM
                     LAECVLLSSIGGVLAIGVAYLGIKALLALKPADLQRPEQVHISLGVLLFTMTVSFAAG
                     IVFGVIPALYVSKTDVNSVLKQSGASQRHPSRIRSALVVGEVGLAVVLLVGAVFMIRS
                     LVSVLNVDPGFRPEHVLTMHFSLPPSHYANKDQMAAFCRQVLERVSALPGVKAASFSD
                     GLPMTRIRMMKFTVDGQPAAKPGSEPTADMRGITSPDYFAAVGIPIVRGRNFTADEID
                     KHLLVIVVNQSLANKLWPNQDVVGKTIVAGARPPDPPLQLTVIGVMGDTHQVSLESGT
                     RPEIVRPMVDYTFLTLTVRGAGDPALMTADIRNQVRGIDKDLPAYDIESMQEIVGQSL
                     GQRRFNSFVMSIFGGLALVLAAVGIYGVLATSVEQRRQEIGIRMALGAQRGDVLRLIM
                     LSGLLLVLIGVGLGIVVGFGLTRWLSSMLYGVSPSSLATYLAVSAAMIAIAMLACYLP
                     AKRATKVDPMKALRAE"
     gene            complement(8479..8961)
                     /locus_tag="VFC15_12505"
     CDS             complement(8479..8961)
                     /locus_tag="VFC15_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM11020.1"
                     /translation="MTKGEFALFRRYEFPKTFKLLRMYPEDKKDLKPSPIIRSAIETL
                     GTFISEEAINLSYARTGAFDVATFKPATPATMAGGIAKLEAVAAETDAALATLSEEDF
                     QKQVDAFGHPMPMSAVMFTMMLDHIHHRGQFTIYSRLAGAKVPQIYGPSADEPMQPPK
                     "
     gene            complement(9014..10063)
                     /locus_tag="VFC15_12510"
     CDS             complement(9014..10063)
                     /locus_tag="VFC15_12510"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZM11021.1"
                     /translation="MAINKRIAVVAGDGVGKEVIPAALDVLRASGAPLECTTFDWGAD
                     RYLIDGTTIPPDGFEMLARDFDAVFVGALGDPRVPSNIHAKEILLGMRFQMDLYANVR
                     PVRLLDASLCPLKNATPDDVNFTVIRENTEGVYSGIGGNFKFGTPDEVATQEDINTRK
                     GVERIIRYAFEYARANGRKKVLMSDKSNVMTYAGDLWQRVFKEVALEFPNIATQHMYV
                     DALCLQMVREPRSLDVIVTNNMFGDIITDLAAALQGGLGMAASGNVHPGRTSMFEPVH
                     GSAPPLAGKNVANPMGAILTGAMMLAHLGLEAEAKKIEGAVLVAVRQKKTTADVGGAL
                     GTRECAQWIATCVAQ"
     gene            10210..11265
                     /locus_tag="VFC15_12515"
     CDS             10210..11265
                     /locus_tag="VFC15_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZM11022.1"
                     /translation="MAVTTFQEKLASAGARLENYRGAETAASFGNSAAEFRALLEGCG
                     LFDMSWQAKLVLTGEDRVRWLNGMVTNNVRDLAVGHGVYNFVLTAQGRIVGDLVAYNR
                     GDFVLITTDRAQSAAIAEIFDRYIIMDEVEVADISDKLTAVGLAGPRAAQVLQAAGID
                     VSQLDPGQVTDLVWHDVGISVAHNTHPNFDAYEIWFAAEQADRVWDELIAAGATPVGS
                     EALELYRIARGVPLFGRDFGQRDLPQETEQKHALNFTKGCYIGQEIVERIRARAILHR
                     TFTGFLVEGEPPQPGTKVQSDGKNIGEFTSAARVPFPSGERTVALGYLRREFEATGTA
                     VQIGEQKATVQSIPFQF"
     gene            11277..11921
                     /locus_tag="VFC15_12520"
     CDS             11277..11921
                     /locus_tag="VFC15_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZM11023.1"
                     /translation="MADKEKKQSEFVVNDRRLFGMDGELRHDVVEAEERREEREQEER
                     DAAQRINEERAAQREAAPIETAAEPPLTPDELHEAPSAAEQQASADAYQETTREVDEQ
                     IRRELGKQGQAHRAKDFEMDFEKFAASLYMSALMQLGLAAPQGGKPQVDMMGARQTID
                     ILGILKDKTKGNLTPAEESMLQNVLYELRMAYLEVTNLIAHPPEGRVPAPFEKR"
     gene            11918..12697
                     /locus_tag="VFC15_12525"
     CDS             11918..12697
                     /locus_tag="VFC15_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM11024.1"
                     /translation="MKAELTVLGSGTSMGVPTIGCDCAVCTSIDPHDRRLRPSIMVQW
                     DGHTVLIDTSPDFREQAIREHINRIDAVLYTHGHADHILGLDDIRPLSFRRVTGGGKI
                     PLYATENTAKVLRSVFRYIFEADYKYGGLAQVEIHNVDGPIELFGAKFVPLVVNHGDY
                     PIEAYRFGSAAYLTDFSTVPDETLAKLEGLDILFLDALRHHPHPTHSTVENSLQIVER
                     LKPKRTFFTHMSHDMPHEETNASLPENVRLSYDGLKLEFEL"
     gene            12712..13656
                     /locus_tag="VFC15_12530"
     CDS             12712..13656
                     /locus_tag="VFC15_12530"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZM11025.1"
                     /translation="MQVFRELEDVPAQLGPTIVSVGNFDGVHMAHQAVVRHMVERAHA
                     IGGKAVVVTFEPHPLRILRPDVAPKLLTPLEVKLQLLEQTGVDAVLVLPFSRDLSLMT
                     AHDFAQQVLVDALHAREVHEGYNFHFGHKAQGNVETLSGFGREMGFDVYTYSEQTLRG
                     HHVSSTEIRALVTAGRVDRARALLGRPFSILSSPGLGRGYGTRYTVPTVNLARYDELV
                     PANGVYITRMRINGECFDAVTNVGVRPTFGPDSFAIESHLLNFHEIELLAQTEVELIF
                     LKRLREEIRFESVEALREQIGRDVARAQRFFRMVTRNQ"
BASE COUNT         2830 a         3799 c         4227 g         2830 t
ORIGIN      
        1 cacgcccacg ctgatagatt cgacgatgtt ctcgttgaac tccttcaagc gctcgtactg
       61 ggcggccttg ctttccagcg atttgtagag gcgcgcattc tgcagggcga ttccgatata
      121 gccggcgagg gtttcgagca attgcacatc ctcactggag aggaagtcgc cttccgcggt
      181 cttgccgagc ccgagcacgg caatcatgcg gttcatcaca gtgcagggaa tgtagtagtt
      241 cagctcgagt cgtcggatgg cttcacgggc ttggggagtt tcgcgtacag cctggctggt
      301 gttgtcgaag aacaagtgtc cggcagccca ttccgggcgt tccacatgca ggaagtccag
      361 gtcgaggccg ccggtgttcg cgatgccgaa ggtcttggcc agcgcgaact gctccgggct
      421 ctccggattc gacaggaaca ctgccattcg gtcgaccatc agggtgcgcg acaggcgatc
      481 gacgatcgag gtgagcatgc tgtcgaggtc ggtttcggaa ttcaggtcgc ggccaaattc
      541 gatcagcgtc tttcggtagt cgtaacgctt tttgtaaaaa agtttgtcga ggtaatcctg
      601 cacccagttc ttgaaaggat cgaatagcag ggcagttacg gcaatcgcaa tcaccaaacc
      661 aaccggaccg gcgctgggga aattcttatg gaacaactcc gaaaccaccc cgacggctgc
      721 aaaatacgcc gccaaaattg cgcctccggc aatggtgtag gccattccgc gtttgaagat
      781 gaggtcgaca tccatcaggc ggtaacggaa tatcgcgtag ccaaatgtca gaggcaggaa
      841 gaccagcgac agcacggaga agcgcatgcc cgcggtcggc acgcttccga gcaggtaagg
      901 aataacgtag aagaccgtaa acggagccac cgcgaggatg gttccgcgcg tgacccactt
      961 catctgctgc cgcaaaatag cggaacccgc gtgtcggtag ctgtgccaca gaacgaaggt
     1021 tgcggtcaag aagtaagcgg cgagatagac catcgccagc cggtcaaggt taaaccgcag
     1081 ggtctcgctg ggttgcagcc acctgacggc gacgatctgc agcgtcagga tcagcgcacc
     1141 cggcaggtac accagaggaa ccagccagcg gcggccggtc agcaaggctt tgcgctcggg
     1201 gaaggtcagc gagaaatgca ggaacagcgc gggctgcaaa agccatgcga ccacattcga
     1261 ccaatagatg atccagtcaa agtcgttgag cttgccggtg tagtggaagg aataaaaaac
     1321 gaaagacacc aggcagaaga cgtagaagtg ggcgctcttg ggcgcagtcc agcggcgcag
     1381 caggacatac agcccaatgc cgagatacac cagcgcaatc aggcgcagac ccaagtacag
     1441 cgaacgatct gcgggagcca ggataagtgg cgcttcgact gggattccct ggcgcaccag
     1501 cgaatacgtg gcttttgacc agatcccggt gcgatataac tgctgctcga ggctggctac
     1561 gttgctgaga ttgcgtccgt caaccgcgac cagccagtcg ccttttttta ctccagcctt
     1621 ctctcccgga ccattcgcgt ccacccgctg tgcctggagg tggtctccgt cttccaccca
     1681 ccacacgcca tcgtatgggg taaagtactc gcggtccttt tggaaattga tcgaggcaaa
     1741 gacgatggcc gcaacgctga agagcgctag tagtaaggcg agaaatcgcg actgtaagtc
     1801 tctggccatc gggctgggcg gctgaatcct aatcgatgcg tgggcgaccg gattctcgaa
     1861 ctcgttcttt gtgtgcaact tagatgccac aatgggggcc atcctgaaaa cctgtaaccc
     1921 ttggtctatg aatagcttcc agaattataa attaacccga agatgagaga tatgtttttt
     1981 ggaatattcg tgtgaaaatc cgaatcgatg gaagttacta aggtggcggt acttacgggc
     2041 caatattgtc tcgcgacaag atgtgtcgta gtgtcgacag accgggtaat ccgggttgaa
     2101 acggcggaaa gtcggcgatt ctccgtgtat ggtaagcagc gatgaacttt gccagtgtcg
     2161 gagcatcagg cctgagccta gccgctgcta cctcctgggg aggaggtgac ttttgcggcg
     2221 gattggcagc caaacgcgcc agcgtgttcc gcgtggttgc ggtcgctcac ggttgtggac
     2281 tgattgccat gctgacaatg gcgtgcctca cgggagaagc gattccgcct cgtgctgatc
     2341 tggagtgggg agcatttgcc ggcctggttg gtgcgttcgg cattgcggca ctctacaaag
     2401 cgctcgcgat tgggcgaatg ggggtggtgg cgccggtggc cgccgtcatc acgggagttt
     2461 tgccggtgct ggtcggcttt cgtaccgaag ggatgcctga cctggctcag ttgttcggtt
     2521 tcgcgctcgc actagtgagc atctggctgg tggcgcgtcc gaatgaggac gtcgactcac
     2581 accgcggcct cgggctagca atattggccg gtgtgatgtt tgggctattt ctggtagccg
     2641 gcaagcaggc ggggcatcac ggagttttct ggcccctggt ggctgcgcgt ttcatctcca
     2701 cattgctgat gcttgctttg gtcgctattt cacctcgaga tccgcagccg ttgcgaaccg
     2761 tgttctggcc gatgatactg tccggccttc tcgactcggc cggcaatgcg ctgttcatcg
     2821 cggccacgcg gcacggtcgc ctcgacgtcg ccgctgtgct ttcgtcactc tatccggcaa
     2881 gcacggtgat tctggcgcgc gttttcctaa aggagcggat ctcaagctgg cagggggttg
     2941 ggattgttgg ggcgctggcg tcggttgcgc tgatctcatg gcgctaagag atggttgaat
     3001 tgaatcgaac agatagcggg gaatgggaca aataacgagt cgaagcgaaa gtcacaaaaa
     3061 atatgaatgg acgcgtgaag ctacttcctc cagatcaaca gccacaacgt cgtcagtaaa
     3121 ccgccccaca acagtacaga tgcggacgct gcaagtaaaa agccgcggaa tgcgctgagc
     3181 tcgtcgttct cgttttcgtg ccgactattt tgctgtggac gtaccggtag tacgtacaag
     3241 ttttcgcctg gttgcccaat cctgaacgca tggcgcatcg agattcagcc cccaagctgc
     3301 gagtaatccg cgaggagaga tcatccggcc agcctcgtag agaactcgca aattccttaa
     3361 cagtgaagcg gggaaagact agatgcaaat cgcaacaatg tgaatagcac agatagggcg
     3421 atcgtttacg ctgctttagt ggtagtccca tgccgtggct gtggtacgag gactctaacg
     3481 cggtattggc gaaatggcaa tttcgtttcg atgcggtgca gaatcgatac cggcgagctg
     3541 gctcggacta tccatgcctc tctaggttct cagggcgcga aaaaggccga agacgattcg
     3601 ctccggcctt tctaccaaag gaagcgttta aagcgcgcga ctagatcttc atcttgttgc
     3661 ggataaggcc tgccaatccc agaacgcccg tgcccagtaa ggcaaggctc gacggctctg
     3721 gaacggaagg tgtgagctgg ccacttgata gattgccctg gtatgtgacg aagtctgcat
     3781 tcaccactgc gccctttggg tagccagtgc tctggaatcc cgcagtcgcg ctggtgatgc
     3841 tgagcgttcc tacgaagacc gcgaggccag gtgtcggaag cgctacggta ttgagcgaga
     3901 gtgtgccgct taatgaattg acgccaatat ttaccgtgaa gttctccgcg ttggccgcca
     3961 gcgtgtagat cccaccaccg ccgccgacta cggcgatggt cgcgttcgcg agggtgaagc
     4021 caggattccc ggagttagca ccttgatagg cccagcccca aacgccggag aatatctctg
     4081 aggttgggct cgctgtgacc gtgcccgccg agctcggtcc aagggttatc tgtgtgggga
     4141 tagggttggc ccacgcgccg gaggcggcca cagtgagcaa agctagacac gcgatcgcca
     4201 gagctaatct cttcattctc gtctcttcct gagcggttgt ccgaccgttc ggaacgctcc
     4261 tttggatgtg ggtacggttg ctcccgacgg gttggaggac cgatatacca cacccatcgc
     4321 ggaagcaagc tcgatgagct acttgcaagt aacatgccaa agtttaccat gataatccaa
     4381 atagttaccg taacgcatgt gcaaacattt gcaataaaag ggaagcaatc ttcccacgtc
     4441 ggagtcgaac ctcgaaattt gctattcacg ccgcggaaac aagcggcctg gcgctgcgtt
     4501 gggatggatt gggagttgcc gcaattagac tgttatctaa attgtcactt tctgcacata
     4561 tattggtgca gatgagcggg ttggctgaat gaagagaaat cggcttggac ggttcgatcc
     4621 agactttcga taagtggcca tttcacagag ctagctaggg tttgcactag agtctagaat
     4681 caacaggaat cgaggccatc atcgtgccga gttaccagga aatcgttgag cttgagcaga
     4741 aatatcttct gaacacctat gcccgttatc cggtggcgtt cgcgcgtggg aagggtgtgt
     4801 atctttttga catcgatggc aagcgctacc tcgactttct ctcggggcta ggggtgaacg
     4861 ctcttgggca cgcacatccc cgcatcgtga aggtcatccg cgatcaggcg gcaaaggtga
     4921 ttcacctgtc gaacctttac tacaacgagt accagggact gctggcggaa aagctttgtc
     4981 aaaagtcggg actcgatcgc gccttcttca ccaacagcgg taccgaggct atcgagggcg
     5041 tgctgaagtt ggtgcgcgcc gccgggcacg ctacggctgg accggagaag atgattgttg
     5101 tcgcgctaga caattcgttt cacggccgca ccatgggagc gctcgctctt accggccaac
     5161 ccaaatatcg ccagggattc gatcccatgc tggaaggcgt gcgcttcgtt ccgcgcaacg
     5221 acatcgaagc gctgcgcgcc gcggttaacg ataaagtttg cggcatcgtg ctggagccga
     5281 tccagggcga gggcggcatt caggtttcgt cgccggattt tctgcatgcc tgtcgcgagg
     5341 tcgcggacca gaacaacgcc gcgcttatct tcgacgagat ccagtgtggc atgggccgta
     5401 cggggcaact cttcgcgttc cagcatttcg gcgtcatacc tgacgtggtc tgcgttgcga
     5461 aaccaatggc cgcaggattt cccatgggag catttctcgc caatgaccgc tttgcgaaac
     5521 acatcacgcc cggcaaacat ggcacgacat tcgggggcgg tcccttggct tgtcgcatcg
     5581 cgctggaata tctggccatc ctcgaagaag aaaatttgct gtaccaagtg cggcgcgtcg
     5641 gagcctactt caccacgaag ctggaggagt tagtggcgaa gttcgatatc gctacggaag
     5701 cgcggggcgt gggcgcgatt caggcgctgc aactcaacat tcccggcaag ccggtgctgg
     5761 acggcgcgct tgccgacgga ctcctgatca acgtaacgca agatagcgta cttcgcttcc
     5821 tgccgccttt cttgctgcaa gagaagcatg tggacgcggg tatgcggatt ctcaagaagc
     5881 aactcgcatc ggcgcagaag cagcataagg cagagatgaa gtcggcgaag cagaagagca
     5941 cggcggcgac agcctagaaa ctcacatgag tgcattcctg caagacctcg ccttcggctt
     6001 tcgcctcatg cggaagtcgc cgctcttcta cctggtggcc gtcctgttac tggcgctcgg
     6061 tatcggagct aacaccgcgg ttttcagcgt ggttgatgcg gtcttgttgc gtaagttgcc
     6121 gttccgcgat cctgacgcgc tggccatggt ctgggaaaag aatccgcaac tcggcgctct
     6181 ggttggcgac cgcgtcccga cgtcctacac gaatttcgaa gagtgggtac ggcaggcgaa
     6241 gtcgtttgat ggcattgccg gattcgaaga tgtcagcctg aatcgtaccg gtaacggcga
     6301 gcccgaacgc gtggcgggtg cgcgggtctc gcccaacttt ttccaggtgc tgggagtaaa
     6361 tcccgcggcc ggaacgacct ttgacttcgt cgagcgcgat cccagccaaa gccacgcagc
     6421 gatgctgagc tatgcatatt ggcagagtca tttcggcgga agccgttcca tcgttggcca
     6481 gaccatcacc ttgaacgacg agtcctacag tgtggtaggc gtgcttccgg ccaatttcta
     6541 tcttccatcg acacggcaag gttcggaaca gcgcaagccc gacatttgga ttccctatga
     6601 tgcttcgcgg aagggtacgg aagccgaacg caatcgccga cgcatgcaag ttttcgcgcg
     6661 actgcgtccg ggcgtgacgc tgcagcaatc gcgtgacgag atgcagacgg tggcgcgccg
     6721 ccttgaggag caaaacactg cgctcaacgc tggattcagc gcgaacgtgt ttcccgtgta
     6781 cgtcgaagac ctgggccagg agttgcggcg caacctgttg gtactgcttt ccgcggtagg
     6841 catggtcttg ctgctggctt gcgccaatct cgcgaacctg atgctgacgc gagccacggc
     6901 gcgacagcgg gaactcgcca ttcgtaaggc cctgggcgcg tcgcgggcgc gattgatccg
     6961 ccagatgctg gcggagtgcg tgctgttgag ttccatcggg ggtgtgctcg ccatcggcgt
     7021 agcctatctc ggcatcaagg cgctgctggc gctcaagccc gccgatctgc agcgtcccga
     7081 gcaggtgcac atcagccttg gagtgctgct gttcaccatg acagtgagct tcgccgcggg
     7141 tattgtcttt ggcgtcatcc ccgcgctcta cgtctcgaag accgacgtca actcggtgtt
     7201 gaagcagtcg ggcgccagcc aacggcatcc cagccgaatc aggtcagccc tggttgtggg
     7261 cgaggtgggc cttgccgtcg tgctgctggt cggcgcggtc ttcatgatcc gcagcctggt
     7321 gtcggtgctc aacgtcgatc ccggatttcg tccggaacac gttctgacga tgcatttcag
     7381 tttgccgccg tcgcactatg cgaacaaaga tcagatggct gcgttttgcc gtcaggtact
     7441 cgaacgggtt tctgcgttgc ccggcgtgaa agccgcgagc ttctccgatg gcctgccgat
     7501 gacgcgcatc cgcatgatga agttcacggt cgatgggcaa cccgcagcca agccgggcag
     7561 cgagccgact gccgacatgc gcggcattac ctcgcccgat tactttgcgg ccgtcggcat
     7621 tcccattgtc agaggccgca acttcacggc cgacgagatc gacaagcacc tactggtcat
     7681 cgtcgtgaat cagtcgctgg cgaacaagct gtggcccaat caggacgtgg tagggaagac
     7741 catcgtcgcc ggagcccggc cgcccgatcc gccactgcaa ctcaccgtaa tcggtgtcat
     7801 gggagacact caccaggtct cattggagag cggcacgcgg cccgagattg tcaggccgat
     7861 ggtggattac accttcctca cgcttaccgt acggggcgcc ggcgatcccg cgctcatgac
     7921 cgcggatatt cgcaatcagg tgcgtggtat cgataaagac ctgccagcgt atgacatcga
     7981 gtcgatgcag gagattgtcg gtcagagcct cggccagcgg cgcttcaaca gctttgtgat
     8041 gtcgatcttc ggcgggctcg ccctggtgct ggcggcggtc ggcatctatg gagtgttagc
     8101 caccagcgtc gagcagcgca ggcaggaaat cggcataagg atggcgctag gagcgcagcg
     8161 cggcgacgtg ctcaggctca tcatgctgag tggactcttg ctggtgctga tcggggtggg
     8221 cctcgggatc gtagtcggct tcggcttgac ccgttggctc tcgagcatgc tctacggcgt
     8281 gagtccaagc agcctcgcga cctaccttgc agtaagcgca gctatgatcg cgatcgccat
     8341 gctggcgtgt tacctgccgg cgaaacgcgc caccaaagtt gatcccatga aggcgctgcg
     8401 agcggagtag ggtgcttccg cctcgggaga cgaatgcttc catagcgtgg tcgccgagta
     8461 ctgatgattg tccatcgttt atttcggcgg ctgcatgggc tcgtcagcgg atggcccata
     8521 gatctgcggt acctttgccc ccgccagacg cgaataaatc gtgaactgcc cgcgatggtg
     8581 gatgtggtcc agcatcattg tgaacattac cgcagacatc ggcatcgggt ggccgaacgc
     8641 atcaacctgc ttctggaagt cttcctccga cagcgtggcg agggctgcgt cggtttccgc
     8701 ggccaccgcc tcgagcttgg caataccacc tgccatggtc gccggcgtgg cgggcttgaa
     8761 ggtggccacg tcgaaagctc cggtgcgggc gtaagagagg ttgatcgcct cctcgctaat
     8821 aaaggtaccg agggtctcga tcgcgctgcg gatgatgggc gacggtttca ggtccttctt
     8881 gtcttcggga tacattcgca gcagcttgaa ggttttggga aactcgtagc ggcggaatag
     8941 agcgaattca cctttagtca tgatgcctcc ttcgaaccgg gttagatgaa gattggctgc
     9001 ctggcggcga attttattgc gccacacagg tcgcaatcca ctgcgcgcac tcgcgtgtgc
     9061 ccagcgcgcc gccaacatcg gcggttgtct ttttctgtcg aacagcaacg agcacggcgc
     9121 cttcgatctt tttcgcttca gcttccaatc caagatgtgc cagcatcatc gcgcctgtga
     9181 gtattgctcc catgggattc gcgacattct tgccggcaag cggcggggcc gagccatgca
     9241 ccggctcgaa catcgaggtg cggccgggat gaacgttgcc gctggctgcc atgccgaggc
     9301 cgccctgaag cgcggctgca aggtcagtga tgatgtcgcc gaacatgttg ttggtcacga
     9361 tgacgtcgag cgagcggggc tcgcgcacca tctgcaggca gagggcgtcc acgtacatat
     9421 gctgagttgc gatattggga aactctagcg cgacttcctt gaagacgcgc tgccaaaggt
     9481 cgccggcgta agtcatcacg ttcgatttgt ccgacatcag aactttcttg cggccgttcg
     9541 cccgcgcgta ctcgaaagca taacggataa ttcgctcgac acctttacgc gtattgatgt
     9601 cctcctgcgt ggcgacctca tcgggcgtgc cgaacttgaa attgcctcca attcctgagt
     9661 acacaccctc ggtgttctcc cggataacgg tgaagttgac gtcgtcgggc gtggcgttct
     9721 tcagcgggca cagcgacgcg tcaagcaggc gcacgggacg aacgttagcg tacaggtcca
     9781 tctggaagcg catgcccaac aggatttcct tggcatggat gttgctgggc acgcgcggat
     9841 cgcctagcgc tccgacgaag acggcgtcga agtcgcgcgc cagcatctca aaaccatcag
     9901 gcggaatggt ggtaccgtcg atgaggtagc ggtccgcgcc ccaatcgaat gtggtgcact
     9961 cgagcggcgc gcccgaggcg cgcagcacat cgagcgcggc ggggataact tcctttccga
    10021 caccatctcc ggcgacgacg gcgatgcgtt tgttaatggc cacggggaat gacaataacg
    10081 aaaagagagg ggaagtagca actaagcaat aagcaatgag cagttggcaa taagcccggc
    10141 ggtccggctg gttgcttatt gctcctcgcg gcagcaacct tttttccccg ttataatcaa
    10201 aggttctgta tggctgtcac tacgtttcaa gagaagctcg catccgccgg agcgcggcta
    10261 gaaaactatc gcggcgccga aaccgctgcc tcgttcggaa actccgcagc cgaatttcga
    10321 gcgctgctgg aaggatgcgg cctgttcgac atgagttggc aggccaagct ggtgctgacc
    10381 ggcgaagacc gcgtgcgctg gctgaacggc atggtcacca acaacgttcg cgacctcgca
    10441 gtcgggcatg gcgtgtacaa cttcgtgcta acggcgcagg gccgcatcgt gggcgacctg
    10501 gtggcctaca atcgcggaga ctttgtgctg atcaccaccg atcgcgcgca gtcggcggcg
    10561 atcgcggaaa tctttgaccg ctacatcatc atggacgagg ttgaggtcgc tgacatcagc
    10621 gacaagctga cggcggtggg cctcgccggg cctcgggcgg cacaggtctt gcaggctgct
    10681 ggcatcgatg tctcgcagct cgatccggga caggtgaccg acttggtgtg gcacgacgtg
    10741 ggaatctcgg tggcgcacaa cacgcatcca aattttgatg cctatgagat atggtttgcc
    10801 gcggagcaag ccgaccgtgt gtgggatgaa ttgatcgcgg ccggtgcgac accggtaggc
    10861 agcgaagcac tggagttgta tcgaattgcg cggggagttc cgctttttgg ccgcgacttc
    10921 ggccagcgtg acttgccgca ggaaaccgag cagaagcacg cgctcaactt taccaagggc
    10981 tgttatatcg ggcaggagat cgtagagcgc attcgcgccc gcgccattct tcaccgcacg
    11041 tttaccgggt tcctggtcga aggcgagccg ccgcagcctg gaaccaaggt tcagtccgat
    11101 ggcaagaata tcggcgagtt taccagcgcg gcacgggtgc catttcccag cggcgagcgc
    11161 acggtggcgc taggctatct gcgccgcgag ttcgaggcta ctgggactgc cgttcaaatc
    11221 ggcgagcaga aggccacggt gcaatccatt ccgtttcaat tttgagcaaa ggacatatgg
    11281 cagacaaaga gaagaagcaa tcggaatttg tagttaacga ccgtcgcctt tttggtatgg
    11341 acggcgagtt gcgtcacgac gtggtggaag ccgaggagcg cagggaagag cgcgaacagg
    11401 aagaacggga cgcggcgcag cgcatcaatg aagagcgggc cgcgcaacgt gaagccgcgc
    11461 caatcgaaac cgccgccgaa ccgccactta cgccggacga gttgcacgaa gcgccttctg
    11521 ccgccgagca gcaggcttca gccgatgcct accaggaaac cacgcgcgaa gtcgatgagc
    11581 aaattcggcg cgagttgggc aagcagggac aagcgcatcg cgccaaagac ttcgagatgg
    11641 acttcgagaa gtttgcggcg tcgctgtaca tgagcgcgct gatgcagctc ggacttgctg
    11701 ctccccaagg cggaaagccg caggttgaca tgatgggcgc gcggcagacc atcgacatcc
    11761 tcggcatcct gaaggacaag accaagggca atctcacgcc ggctgaggag agcatgttgc
    11821 agaacgtact ctacgagctg cgcatggcgt atctcgaagt gacgaatctc atcgcgcatc
    11881 cgcctgaggg tcgagtgcct gctccgtttg aaaaacgatg aaagcggaac tcaccgtgct
    11941 gggcagcggt acctcgatgg gcgtgccgac gatcggctgc gactgcgcgg tatgcacctc
    12001 gatcgatccg catgaccgcc gtctgcgtcc ttcgatcatg gtgcagtggg acggtcacac
    12061 tgtgctgatt gatacctcgc ctgacttccg cgagcaggcg attcgcgagc acatcaatcg
    12121 cattgatgcc gtgttgtaca cgcacggaca tgccgaccat attcttgggt tggacgatat
    12181 tcgtccgctg agttttcgcc gcgtcaccgg cggagggaag attccgctgt acgcgacgga
    12241 gaataccgcg aaggtgttgc ggagcgtttt tcgctacatc ttcgaagcgg actacaagta
    12301 cggcggtttg gcgcaggtcg agatccacaa tgtggatggt ccgatcgaat tatttggcgc
    12361 taagttcgtt ccgctggtgg tcaatcacgg cgactaccca atcgaagcgt atcgctttgg
    12421 ttcggcagcg taccttaccg acttcagcac ggtgccggac gaaacgctgg caaagctcga
    12481 aggcctcgac atcttgtttc tcgatgcgct gcggcaccat ccgcatccca cgcactcgac
    12541 ggtggagaat tcactccaga tcgtcgagcg gctcaaaccc aagcgcacat ttttcaccca
    12601 catgtcgcac gacatgccgc acgaggagac caatgcctcg ttgcccgaga acgtgcgcct
    12661 ctcctatgac gggctcaagc tcgaattcga actctagggt gccgaccgac gatgcaagta
    12721 ttccgcgagc ttgaagatgt tccagcgcaa ctaggcccga ccattgtgag cgtgggcaat
    12781 ttcgacggcg tgcacatggc acaccaggcg gtagtgcggc acatggtgga gcgcgcgcat
    12841 gccatcggtg ggaaagcggt cgtagtgacg tttgagccgc atccgctgcg catcctgcgg
    12901 cctgacgtcg cgcccaagct gctgacaccg cttgaggtca agctgcagtt gctggagcag
    12961 actggtgtgg atgccgtgct ggtactgccg ttttcgcgcg acctgtcgct gatgacggcg
    13021 cacgatttcg cgcaacaggt gctggtggat gcgctgcacg cgcgcgaggt gcatgagggc
    13081 tacaatttcc atttcggaca caaggcgcaa ggcaacgttg agactctctc tggattcggg
    13141 cgcgagatgg gattcgacgt ttacacctac tcagagcaaa ctctgcgcgg acaccacgtg
    13201 tcgagcacag aaattcgtgc gctggttacc gctggacgag tggaccgcgc gcgcgcgttg
    13261 ctgggacggc cattcagcat cctgtcgagc ccgggcctcg gacgcggata cggaacgcgg
    13321 tacacggttc cgacggttaa cttggcgcga tacgacgaac tggttccggc caacggcgtg
    13381 tacatcacgc gcatgcgcat caacggcgag tgcttcgatg cggtgaccaa tgtgggtgtg
    13441 cgtccaacgt ttggacccga ctcgttcgcg atcgagagcc acctgctcaa ctttcacgag
    13501 atcgagttgc tggcgcaaac ggaagtcgag ttgatctttc tgaagcggct gcgtgaggag
    13561 atcaggtttg aatcggtgga agcgctgcgc gagcagatcg gacgagacgt tgcgcgagcg
    13621 cagaggttct ttcggatggt gacgaggaac cagtaattag taattgataa tcggcagtta
    13681 gctaag
//
LOCUS       DASRKK010000543         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_632791, whole genome shotgun sequence.
ACCESSION   DASRKK010000543 DASRKK010000000
VERSION     DASRKK010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632791"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..391
                     /locus_tag="VFC15_12535"
     CDS             161..391
                     /locus_tag="VFC15_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11026.1"
                     /translation="MGRDTSNDVHVVLNHKFCFYGVAAIYKVRKTSRGTSVAEICGGI
                     PPHIEALSKRVQNLQDIFSIQSKSAFLGHRSL"
     gene            complement(1252..1395)
                     /locus_tag="VFC15_12540"
     CDS             complement(1252..1395)
                     /locus_tag="VFC15_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11027.1"
                     /translation="MSQSQRKTVVDYIDRQAEHHAKWSFEQEFVTMLKKSGIPYDPRF
                     VFG"
     gene            complement(1623..>2103)
                     /locus_tag="VFC15_12545"
     CDS             complement(1623..>2103)
                     /locus_tag="VFC15_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11028.1"
                     /translation="GVCNPDVLLLLPLLARPHCHAHILRTKPVDDSFILGRHPEFHHR
                     LLGTAETNIAICQRSPQTLIPPKYLRLMAHTHTCNLVHFVFSTKDRASLIPDPERLWQ
                     YVGGIARQKHILLLAAGGTANHLHLLISLPPTITLAKAVQEVKGNTSRWLNETSRFA"
BASE COUNT          469 a          572 c          596 g          466 t
ORIGIN      
        1 gcaggatcac ctggcatttg cgggccgtct tttgtggcgt agtgcccgcc cgagccatcg
       61 attccaattg acttttctgt gcctcatcga catgcagagc aggggcgcga acaaacatgc
      121 ctctattgga tcacgaatcc gcattattgt aaataattaa atgggacggg acactagcaa
      181 tgacgtccat gtagtgctga accacaagtt ctgcttctat ggcgttgccg caatctacaa
      241 ggtccgaaag accagtcgcg gtacctccgt cgcggagata tgcggcggta ttccgccaca
      301 cattgaggcg ctcagtaagc gcgtccagaa tctgcaagac atcttctcga ttcagagcaa
      361 gagtgcattt ctcggtcatc gttcactcta gaatgagggg ttcctaaacc ccgatattga
      421 gaatggttga caagggaaac ctgcgacgag aatgtcatga gggggtagat cctctgatgc
      481 tatggcgcgg agatcaccgt gcggcttttc gccaaagttc gcttcatatg ttatctgggc
      541 gtacttatcc cagtcggatg agaaaacgca ttgggcaccg gcagattcga aggccaaccg
      601 catcccgccg attccgcaaa ataagtcgat aaacttggga cagttcacgt tgtcgcaccg
      661 cacttgtaag ccgaagataa cacagctcgg cgcaaatgtg agtggcgttc tgccgcgtgg
      721 cccatcctcg tccgctgctt ttgcggacag gatgggaggg gacgatgagc cccgctggta
      781 atcacgccca cgcaatggtg ccgcccgctg aagccggctc cgaattggta taaccagccc
      841 acccaggact tacgtcctgg gctaattttg ttccgccctg acgggctcgc tcctgcgcat
      901 agttatcaat tccgctctcc ccaagctgcg aggattacac gttgactcgg agggaggtgt
      961 cacataaagt gtggccttcg aaccacccca gcgagcgcag aaccgccgct tgtcggcgcc
     1021 gcggtaagat cactcgtagc atcccggaag caagcgaggt cacgcggcaa tggcgaaccc
     1081 tgaacatcta gaaattctgg agatgggctg tcctcgaact gctggtgcgg gtcgggcgtt
     1141 ggagccacca cgcgcgagtc ccgcggagcg ggacgaaaga gcttagccca gcctttcaag
     1201 gctgggaaag cagcgtaata aatccgagtc ccgtagggac ggcagaggaa gctagccaaa
     1261 tacaaatctc ggatcgtagg gaattcccga tttcttcagc atcgtcacaa actcctgctc
     1321 gaagctccat ttcgcgtgat gctcagcctg gcggtcgatg taatccacca ccgtcttgcg
     1381 ttgcgactgg ctcacacgag cagattctga gttggtgtat ccgggtggag caccgcttca
     1441 gcggtgcatt tgacagttgc ctctgaattc ggctttagcc gctgaggtaa ccagagctct
     1501 tacctcagcg gctaaagcct cgcccttttt gcagccttgg tatgcacgcc tgaaaggcgt
     1561 gctccaccca acatcggata cagcaactcc ggaatcgctc taaacgcgct gtatccctcc
     1621 tgctacgcaa accgggacgt ttcattcagc catcgtgagg tatttccttt gacttcctgt
     1681 acagccttgg ccagcgtgat ggtcggcggc agcgagatga gcagatgaag gtgattagcg
     1741 gtgccaccgg cagccagcaa cagaatgtgc ttctggcgcg cgattcctcc gacgtattgc
     1801 catagtctct cgggatcggg aatgaggctg gcccggtctt tggtgctgaa tacgaagtgc
     1861 accaggttgc aggtgtgagt gtgcgccatc agccgaagat acttgggtgg aattagagtt
     1921 tgcggtgatc gttggcatat tgcgatgttt gtctctgccg tccctagcag cctgtggtga
     1981 aactctggat gcctgcccaa aataaatgag tcatccacag gcttggttcg tagaatgtgc
     2041 gcatggcaat ggggacgcgc aagcagcggg agaagcaaga ggacatctgg attgcacaca
     2101 ccg
//
LOCUS       DASRKK010000544         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_828663, whole genome shotgun sequence.
ACCESSION   DASRKK010000544 DASRKK010000000
VERSION     DASRKK010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828663"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            709..831
                     /locus_tag="VFC15_12550"
     CDS             709..831
                     /locus_tag="VFC15_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11029.1"
                     /translation="MSVLFRFTPAAAAGSFDANSFAAPQARAALAGHEINPAFP"
     gene            858..1238
                     /locus_tag="VFC15_12555"
     CDS             858..1238
                     /locus_tag="VFC15_12555"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HZM11030.1"
                     /translation="MGRHRPKQDHTLENLLDLDGDIYVIDETAGYWVKFVVRCVIPSP
                     ERPHGVEYSLTMHDSSNRRLVGFDNAHPVPGAKRPQRGTDRAYDHRHRMGAVKPYPYE
                     NAAKLVADFWKTVDEVLKLKGDGP"
     gene            1235..1594
                     /locus_tag="VFC15_12560"
     CDS             1235..1594
                     /locus_tag="VFC15_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM11031.1"
                     /translation="MTTLRVGIATYQQMKERTLAIAQGKLKPGPHDPKVWFTSTESMA
                     RILSAKNRELLKIIRKNSPQSLAELAEQTGRRKPNLSRTLKTMEHYGLVKVSRQNRRL
                     IPRVDYQRVAVVMELSA"
     gene            complement(2022..>3636)
                     /locus_tag="VFC15_12565"
     CDS             complement(2022..>3636)
                     /locus_tag="VFC15_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZM11032.1"
                     /translation="ALRRTAFSPNIKERRDYSCALFDAAGNVLAMGDHMPVHLGSMPM
                     SVRTAIAELRLAPGDVAMLNDPFRAGTHLPDITVVAPVFVGRARCDAAPEFFVAARAH
                     HADVGGTYAGSMGPCREIYQEGFRIPPVKIVRGGTLDRDILALLLNNVRTPEEREGDL
                     SAQLAACHTGSARLLELCARYGIARVQRAARALLDYSEKMMRAFLATVPPGCYAAEDV
                     LDDDGVTDEPVRIAVRITFPRATKIASRTPRPVVIDFTGSSPQVEGSINAVEAITYSA
                     CFYVFRCLLREDVPATAGLMRPIQLIAPEGTIVNARPPAAVAGGNVETSQRIVDVLLR
                     ALAQALPEHIPAASSGTMNNLTIGGMDDRSGKAIPFAYYETIAGGSGASAAHDGVSGV
                     HTHMTNSLNTPAEALEYSYPLRVMKYALRAGSGGEGKHRGGDGIVREIEVLADATVTM
                     LADRRKSRPYGLAGGEDGAPGRTEILRADGSIELMPSKGSTRLRRGERVRIETPGGGG
                     WGAAQGLRASPEGAPVQNASADHTEPFTR"
BASE COUNT          733 a         1074 c         1118 g          711 t
ORIGIN      
        1 atggtgtaca tggtttgaaa aaattgggaa caagcaggaa cttcacagag agcactttaa
       61 ccctgtgacg ctggagaaat ggcagcggcc tagcggtaca tttgccatca ggcggggata
      121 gttccacgtc ggatcaggtt ccttttggca ccaggggaac ggcccactct cacattctgc
      181 gcgtgcctgg tgccccaggt tcgcgtccgt gttttggacg ttgacctggg ctgaggaagg
      241 tccgggccga cccacccaac attttttacc cacgatctct ggccgtccac tgcgattcga
      301 tctccaccac tccgttctcc cgcaaagcgt agttattggt gggagccccc gcatttatgc
      361 gggggaaaaa agttcgtctt ctcctcacgc gctttagcgc cgggtcgaga aatcccgggg
      421 ccgaagccca tttgaagatg gaaatgctct tccgctggac tgaagtccag cttccccctg
      481 ctgaagcagg gggcttccac caaaagtctt aagagcaggt ccctcgactg ggactgaagt
      541 cctcgctcgg gatgacaaag agcagggact agtggctagg ggtcaggcat tagtaaaggc
      601 ggaatccaca agaacagggg ctagtggctg ggggtcaggg attagtaaag gcgcccgaac
      661 tattttcgct cttttccgtt cggccgcggc tccaaggaaa cgcgcaagat gtcagtcctg
      721 ttccgcttta ccccagccgc cgccgccggg agtttcgatg cgaactcgtt cgccgcgccg
      781 caagcgcgtg ctgcccttgc tgggcatgag atcaatccgg catttcctta gttatcacct
      841 tctgataata tgatgctgtg ggacggcatc ggcccaagca ggaccataca ctcgagaacc
      901 tgcttgatct tgacggagac atctacgtca tcgatgagac tgccgggtac tgggtgaagt
      961 ttgtggtcag gtgcgtcatt cccagtccgg agcgtcctca cggagtcgaa tactcgctga
     1021 cgatgcacga ttccagcaat cgacggctgg tcggcttcga caacgcgcat cctgtgccgg
     1081 gggcaaaacg gccccagcgc ggcactgacc gcgcttacga tcaccggcat cgaatgggtg
     1141 cggtcaaacc ttacccatac gagaacgccg caaaactagt cgcggatttc tggaaaacag
     1201 tcgatgaagt gctcaagctg aaaggggacg gaccatgaca acgctaagag tgggaatagc
     1261 cacataccag cagatgaaag agcggacctt ggccatcgct cagggaaaat tgaagcctgg
     1321 accacatgat cccaaggtgt ggtttacctc gacggagagc atggcgcgga tactttccgc
     1381 gaaaaaccgc gagctgctga agatcattcg caagaattcg ccgcagtcac ttgccgaact
     1441 ggcagaacag accggacgca ggaagcccaa tctgtctcgc actctgaaga cgatggagca
     1501 ctacggtctg gttaaggtgt cgcgacagaa ccgccggctc attccgaggg tggactacca
     1561 gcgtgtagcg gttgtcatgg agcttagcgc ctagagaagc ccgcctggtg ccccaggttc
     1621 gcgtccgtct tttggacgtt aacctgggca ggggaaggtt cgggccggcc caccggacat
     1681 tttcaaatca cgatctctgg ccgtccactc cgattcggtc tctaccattc cgttctcccg
     1741 caaagcatag ttattggtgg gagcccccgc atttatgcgg gggaaaagtt cgtgttttga
     1801 tcgcgcgctt tagcgccggg gctcgataaa tcccagggct gaagcccatt tgaagatgga
     1861 atgctcttcc gctggactga agtccagctt ccccctgctg aagcaggggg cttccaccaa
     1921 aagtcttaag agcaggtccc tcgactcgga ctgaagtcct cgctcgggat gacaaaaccc
     1981 agttgccagt tgccggttgt cagtaaaaca agacccaatc actaacgagt gaagggttca
     2041 gtgtgatcgg cagacgcatt ttgaacgggc gcaccttcag ggcttgctct gagcccttgc
     2101 gccgctcccc agccgccgcc gccgggggtt tcgatgcgaa ctcgttcgcc gcgccgcaag
     2161 cgcgtgctgc ccttgctggg catgagttcg atggaaccgt cggcgcgaag tatttcggtc
     2221 cgtcccggcg ctccgtcctc gccgccggcg agaccgtacg gtcgagactt gcgccggtca
     2281 gcgagcatgg tcaccgtggc gtcggcgagc acttcgattt ctcgcacgat gccgtcgccg
     2341 ccacgatgtt ttccttcccc gccgctaccg gcgcgcagcg catacttcat cacccttaac
     2401 ggataactgt attccagcgc ttccgcggga gtgttgagcg agttggtcat gtgcgtgtgc
     2461 acgcccgaga cgccgtcgtg ggccgcgctg gcgcccgagc cgccggcaat ggtttcgtag
     2521 tacgcgaaag gaatggcctt cccgctgcga tcgtccatgc cgccgatggt gagattgttc
     2581 atggtccccg acgacgcggc ggggatgtgc tcgggcagcg cctgggccag cgcgcgcaga
     2641 agcacgtcaa cgatgcgctg cgaggtctcg acatttccgc ccgccacggc tgcgggggga
     2701 cgcgcattaa cgatggttcc ctcgggagcg atcaactgaa tcggtcgcat caatccggcg
     2761 gttgcgggaa cgtcttcgcg cagcaggcag cggaagacgt agaagcatgc tgaatacgta
     2821 atggcttcca cggcgttgat gctgccctcg acctggggcg agctgccggt gaagtcgatg
     2881 accacgggtc gcggcgtccg gctcgcgatt tttgttgcgc gcggaaacgt gatgcgcaca
     2941 gcaatgcgca caggttcgtc ggtgacgcca tcgtcatcaa gcacgtcctc ggccgcgtag
     3001 catcccggag gcaccgtcgc caagaatgcg cgcatcatct tctcggagta atccagcagc
     3061 gctcgcgcgg ctcgctgaac tcgcgcgatg ccgtaacgtg cgcaaagttc gagcagtcgc
     3121 gcgctgccgg tgtgacacgc cgccagctgc gcgctgaggt cgccttcacg ttcttcggga
     3181 gtgcgaacat tgttaagcaa cagggcaagg atgtcgcggt cgagagttcc gccgcgcacg
     3241 atcttcaccg gcggaatgcg aaatccttcc tggtagatct cgcgacacgg gcccatcgaa
     3301 ccagcgtagg tcccaccgac atcggcgtga tgcgcgcgag cagcgacgaa aaattcggga
     3361 gcggcatcgc accgggcgcg tcccacaaac accggagcga cgacagtgat gtcgggcaga
     3421 tgcgtgcctg cgcggaaggg atcgttgagc atcgccacat cgccgggcgc gagccgcaac
     3481 tccgcaatgg cggtgcgcac cgacatgggc atcgagccga gatgcacggg catgtgatcg
     3541 cccatggcaa gcacgttgcc ggctgcgtca aagagcgcgc aggagtagtc gcgacgctct
     3601 ttgatgttgg gcgagaaggc cgtgcggcgc agcgct
//
LOCUS       DASRKK010000545         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_340252, whole genome shotgun sequence.
ACCESSION   DASRKK010000545 DASRKK010000000
VERSION     DASRKK010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340252"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1863
                     /locus_tag="VFC15_12570"
     CDS             409..1863
                     /locus_tag="VFC15_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZM11033.1"
                     /translation="MVTNRIQHSDKKPVKQGSGAMAPEPNKIGASTPIDFSGKNLTPY
                     GGLLPVATMLEKLGFQALMEEAVMVKRLTKVMSGYQFLLAMVLGLYIGFSRLNQLRFV
                     ARDPMLTGVLKIVRLPPQCTLWRFLAALHGSVAQQILWVQGQMRRRVWEAANVKLKTA
                     TLDTDTTVHTLYGQQMGARKSYNPKNKGKKSYQPILTFLAETREYLAGELRNGDRPTG
                     DQIARHLRSAFQSLPAGVEKIFARADSGFYCWGAVEAYQKAGCRFIVVARKTSRLVEE
                     LNQAQWKPSPKTDGDAECEFRYQPDGWGQAFRFVALRYEKKADEVEAGEAEQYQLFET
                     SRYTYRVFVTDLEEPIDLAVWFYNQRAGAENLIKEANNDAGLAAHPSKRFDVNGNHFQ
                     LAMLAYNLNCWLMLFNREAGADTGTLRHTTLATARLRFLFVAAKIWRHAGRTGVSYSD
                     HYEERGLFQQLMDRLRAIAGRGPGFGPVVAAALT"
     gene            2012..>2205
                     /locus_tag="VFC15_12575"
     CDS             2012..>2205
                     /locus_tag="VFC15_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11034.1"
                     /translation="MDSDQPPGRRAAAHATGAPSRVFVHNSGDKKPVKQGSGAMAPEP
                     NKIGASTPIDFSGKNLTPYGG"
BASE COUNT          461 a          631 c          702 g          411 t
ORIGIN      
        1 gttttgacat cgtccctacg ctttcgacac ttccatagtg gttcccttgc ggtcctcatt
       61 cttgcctgtc acctgacctg gtccttgcca ggccttttcc ttctgtgctc acgaccatgg
      121 cttttgacca cagccgccga aggtggtttg gaacctgctc ctgccagccg gttccgaggg
      181 accttccctc atcgatcaag cagttacaca cattgggccc tcctcggccc tttgcgctcg
      241 tggcgcacgg tcatccgcgt accgttcaac ctgacctatg caccgcagca ggcatgcggc
      301 cggcgttgaa ctgagaccca accatttggg gaccccgtta tcatgggatg aaactgaaac
      361 gtggcgaagt tgcgagcttc gcaagccaac cccatggagg tcacccaaat ggttacgaac
      421 agaattcagc atagcgacaa gaagcccgtt aaacaaggct ctggagcgat ggctccggaa
      481 cccaataaaa tcggggcttc cacgcccatc gacttttccg gtaagaacct gactccgtac
      541 ggaggcctcc tacccgtggc caccatgctg gagaaactgg gcttccaggc gctgatggag
      601 gaggcggtca tggtgaagcg gctcaccaag gtgatgagcg gctaccagtt cttgcttgcc
      661 atggtgttgg gcctgtacat cgggttcagt cgcctgaacc aactgcggtt cgttgcgcgc
      721 gatccgatgc tgacgggcgt tctgaaaatc gtccggttgc cgccgcagtg cacgctgtgg
      781 cgctttctgg cagcgctaca cgggagtgtg gcccagcaga ttctatgggt gcaaggccag
      841 atgcgccgcc gcgtatggga agcggcgaac gtgaagctga agaccgcgac actggacacc
      901 gatacgacgg tgcacacgct gtacgggcag cagatgggtg cgcgcaagag ctacaacccg
      961 aagaacaagg ggaagaaaag ctaccagccg atactgacgt ttctggcgga gacgcgggaa
     1021 tacctggcgg gcgagttgcg caatggcgac cggccgacgg gggaccagat cgcccgccat
     1081 ttacgcagcg ctttccagtc gctgcccgcg ggggtggaga aaatcttcgc gcgcgccgat
     1141 tcgggcttct actgttgggg ggccgtggaa gcctaccaaa aggcgggctg ccggttcatc
     1201 gtggtggcgc gcaagacctc gcggctagta gaagagttga accaggcgca atggaagccg
     1261 tcgccgaaga ccgacggcga tgccgagtgc gaattccgct accagccgga cggatggggc
     1321 caggcgttcc gcttcgtggc tctgcgctac gagaagaaag cggacgaagt ggaggctgga
     1381 gaggctgagc agtaccagtt atttgagacc agccggtata cctaccgggt cttcgtgacc
     1441 gacctggagg agccgatcga tctggcggtg tggttttaca accagcgggc gggggcggag
     1501 aacttgatca aagaagccaa caacgacgcg ggcttggctg cgcacccctc caaacggttc
     1561 gatgtcaacg gcaatcactt ccagttggcg atgttggcct acaacctgaa ctgctggcta
     1621 atgctgttca accgggaggc cggcgcagac accggcacgt tgcgtcacac cacgttggcg
     1681 acggcgcggt tgcggtttct attcgtggcg gcgaagatct ggcgtcatgc gggaagaacc
     1741 ggagtgagct acagcgacca ctacgaagag cgcggcctgt tccagcaact gatggaccgg
     1801 ctgcgcgcga ttgcggggcg tggtcccggc ttcgggccgg tggtggccgc cgcgctgacc
     1861 tgaggaccgc cgtgcataac ttttcatgca cgactcggaa ctgcaagcag gaacacaaca
     1921 tagcagggag aagttgctga gatgcaaagc cgggagtcga gggaaatgag agagcggagg
     1981 cgttggttga cttaataagg ggacgatcag agtggattca gatcagccgc ccggccgccg
     2041 cgccgctgct cacgccaccg gtgcgccgag tcgggttttc gtgcataatt caggcgacaa
     2101 gaagcccgtg aaacaaggct ctggagcgat ggctccggaa cccaataaaa tcggggcttc
     2161 cacgcccatc gacttttccg gtaagaacct gactccgtac ggagg
//
LOCUS       DASRKK010000546         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_437904, whole genome shotgun sequence.
ACCESSION   DASRKK010000546 DASRKK010000000
VERSION     DASRKK010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437904"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..903)
                     /gene="lexA"
                     /locus_tag="VFC15_12580"
     CDS             complement(265..903)
                     /gene="lexA"
                     /locus_tag="VFC15_12580"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZM11035.1"
                     /translation="MALTRRQKQVYDFISEFVDKHGYSPSFEEIAEGLGLSSLATVHK
                     HITNLEQKGLLRRDYNRSRSIDLLKPRGRMKQSLAVAAAAAAESGMSLPLMGRIAAGR
                     PLEAMENPESISLADFTRSKDVYVLQVTGESMQDEHIVDGDYVLVEKTNVARDGEIVV
                     ALVNGSDATLKRIYLEGNKIRLQPSNAAMQPIIVPAVTVQVQGRVIGVLRKY"
     gene            complement(980..1603)
                     /locus_tag="VFC15_12585"
     CDS             complement(980..1603)
                     /locus_tag="VFC15_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11036.1"
                     /translation="MPRNLAPAALGFRVHSGWAAVVAVSGPLTSPAVIERRRIEIAAP
                     EIPGSKQPYHAAEKLGLKEAEQFLLRCHQSSVSLARKAIGDCIHDANRKGQNVARCGI
                     LFASGRPLGTLAAILASHALIHTAEGEFFRNVVLDACKHHEVQAIGVKEKELLPHFEN
                     EIHISQEALNQYLSSIGRIIGPPWRQDERLAMMVAWLALAAAGRTSN"
     gene            complement(1605..>2544)
                     /locus_tag="VFC15_12590"
     CDS             complement(1605..>2544)
                     /locus_tag="VFC15_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM11037.1"
                     /translation="GSVACLQNVTTCQATVASCFDFSGRRNKVVYTDLNFPSVMYFWE
                     AQRSRGARVEMVRNDDGIHVNTERLLAAIDEETLIVPISHVIFRSAFIQNVEAIVEKA
                     HRVGAYVLLDSFQATGTVPFDVQKLKVDFCTGGVLKWLCGGPATAYLYVRPDLAKKLE
                     PTFTGWNGHENPFGFEIGATCYSPTIYRFTQGTPNIPALKVAQPGLKIIAEAGVEKIR
                     EKSKRQTALLIAMADRHGWRVNTPRDAERRGGTVSIDMPNAQQVCAELLKRDVLVDYR
                     PKAGVRFSPHFYNTDEELTKGIAAVEEILGEMNVGA"
BASE COUNT          519 a          742 c          745 g          538 t
ORIGIN      
        1 taggggtgcc actatgatca aaatgtttca agcacccgag ggattaaggt gcaatgggaa
       61 tgccaaaagg ggaggaaagg aaacactagg gttatgtggc tggaccagcc caggagcggg
      121 aaacttttac caattgtgag agaagcctgc catggttgat ctgaagagcc gaaactagct
      181 aaaacgcggg gcagagtgaa cgccaaaggc gcccagcaaa ttgcttacgc ataatggtcg
      241 ctttctggtt tttctctcgc cacttcagta cttccgcaga actccaatca ctcttccctg
      301 tacctgcact gtgaccgcgg gaacgatgat gggctgcatg gcggcattcg aaggttgcag
      361 gcgaattttg ttgccctcaa ggtagattcg tttcagcgtg gcgtcgctgc cattcaccag
      421 cgcgaccacg atctcaccat cgcgcgcgac attggttttc tccaccagaa catagtcgcc
      481 atcgacgatg tgctcgtcct gcatgctctc gcccgttacc tgcagcacgt acacgtcttt
      541 ggagcgggtg aagtcggcga gcgaaatgga ctcgggattc tccatcgcct ccagcggacg
      601 gccggcggcg attcgtccca tcagcggcag gctcatgccg ctctccgccg ccgctgccgc
      661 agccacggcg agcgactgct tcatacgtcc ccgcggcttc agcagatcga tggagcggct
      721 gcgattgtag tcgcggcgca acaatccctt ctgctccagg ttggtgatgt gcttgtgcac
      781 ggtcgccagc gagctgaggc cgagcccctc ggcaatctct tcgaacgatg gcgagtagcc
      841 gtgcttgtcc acgaattcgg agataaagtc gtacacctgt ttctgacggc gagtgagtgc
      901 catggtcgtg cgcagccctc cgccagtatt gtcagcgaac aaaaggcgaa aagtcaacag
      961 agaatttcaa cttggtgatt caattcgagg tacgtccagc cgcggcgagt gccagccaag
     1021 ccaccatcat ggcgagtctc tcgtcctgtc gccacggagg accgatgatg cgcccaatcg
     1081 acgaaaggta ctggttcagg gcttcttggg agatgtggat ctcattctcg aaatggggca
     1141 gcagttcttt ttccttcact ccgatcgctt gcacttcatg atgcttgcaa gcgtccaaga
     1201 ccacattccg aaagaactct ccttcggcgg tgtgaatcag tgcatgagag gcgaggatgg
     1261 ccgcaagggt tccaagagga cgtccggaag cgaatagaat cccacagcgg gctacgttct
     1321 gaccctttct gttcgcgtcg tgaatgcagt caccgatagc tttcctcgcc agcgaaaccg
     1381 agctttggtg gcagcgcagc aagaattgct ccgcctcttt cagccccagt ttttcggcgg
     1441 catgatacgg ctgcttcgag ccggggatct cgggagcagc aatttcgata cgccgtcgct
     1501 caatcaccgc aggtgaagtc agaggtcccg aaaccgcaac tacggctgcc cacccggaat
     1561 gtacgcggaa gccgagggcc gctggcgcca aatttcgggg catactacgc tcccacattc
     1621 atttcgccca agatctcttc cactgcggcg atcccttttg tcagctcttc gtcggtgtta
     1681 tagaaatgtg gcgagaagcg cacgcccgcc ttggggcggt agtcaaccag tacgtcgcgc
     1741 ttgagcagtt cggcgcaaac ctgttgcgcg ttgggcatgt caattgacac ggtgcctccg
     1801 cgacgttcgg cgtcgcgcgg cgtattcacc cgccagccgt ggcggtcggc catcgcaatg
     1861 agcagcgcgg tctgccgctt cgacttctcg cggatctttt cgacaccggc ttcggcaatg
     1921 attttcagtc ccggctgcgc aactttcaga gcgggaatgt ttggcgtgcc ctgggtgaag
     1981 cgatagatgg ttggcgaata gcacgtcgcg ccaatctcga acccaaaggg gttttcgtgg
     2041 ccgttccatc cagtgaatgt cggctcaagc ttcttcgcca gatcgggacg cacatacaga
     2101 tacgccgtgg ccggcccgcc gcacagccac ttcagcacgc cgccggtgca gaagtccacc
     2161 ttgagctttt gcacatcgaa gggcacggtg cccgtcgctt ggaaggaatc gagcaggacg
     2221 taagcgccga cgcggtgcgc cttctccacg atggcctcca cattctggat gaaggcgctg
     2281 cgaaagatta cgtgcgagat agggacgatg agcgtctctt cgtcgatcgc cgcgaggagg
     2341 cgctcggtgt tgacgtgaat gccgtcgtcg ttgcgtacca tctcgacccg agcgccgcgt
     2401 gaccgttgcg cttcccagaa gtacatcacc gagggaaaat tcaggtcggt atagacgact
     2461 ttgttgcggc gcccgctgaa gtcgaagcac gaagccaccg tcgcctggca ggtggtcacg
     2521 ttttgcagac aggcgaccga gccc
//
LOCUS       DASRKK010000547        34165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1317399, whole genome shotgun sequence.
ACCESSION   DASRKK010000547 DASRKK010000000
VERSION     DASRKK010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 34165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34165
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317399"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3915
                     /gene="gltB"
                     /locus_tag="VFC15_12595"
     CDS             <1..3915
                     /gene="gltB"
                     /locus_tag="VFC15_12595"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZM11038.1"
                     /translation="DAHEIERRLYFARKAFERGGTDTYVCSLSTRTLVYKALCTGRTL
                     HEFYPDLQNKQYATHFSVFHQRYATNVLPSWDRAQPLRMVAHNGEINTIWSNRAHVDA
                     RCATLPSECDPIYTPDASDSLSLDEVAEMLSNYERNVAEAVRMMLPPADGPKESAFHR
                     YHADCMEPWDGPSALVFTDGRLLGAALDRNGLRPCRFVITDTGLVVAGSEVGLADVDS
                     DTIIRSGRLGPGQMILLDLERCRLIEGSELDRYFNDLAPYAKLIDDIPLEPSGTPVSA
                     LDKDDLTRLQRGFGYTREEVKMVVTPMAMESKEAIWSMGDDTPIAPLARTPRPLYGFF
                     RQRFAQVTNPPIDSLREACVVSSRTRLGPWPHLLDKHAPLPGLVLPTPFLSLAQMAAL
                     HEGTYALHNELPLAHLNCTFADDSSLQQALMELQKHAVELVRGGVCILLMTDRTACEH
                     LLPIPMAMATGAVHQALVKAGLRTKVGLALEAGDCRDVHHAAVLIGYGAGSVCPWLAL
                     QTARTLAGEEGETRLLNALNSGLAKVMSKMGISVLDSYRAAQLFDVLGLDSGITDLCF
                     PGTVSPIGGQNFADIELAIRSSWIAEPETTELPDYGWVRFRRSDRSEPHTWQPQRTRD
                     LQVAVGAARASTQAATTIAPAEAWSAYSRAVDEREPAVLRDLLQIKAAGPKLELHEVE
                     PPSSLVKRFIASAMSLGSLSPEAHQTITIAMNQMGARSNTGEGGEDPVAYKPDGNTSG
                     VLSLLNNKIKQVASGRFGVTAEYLAHAEELEIKIAQGSKPGEGGQLPGHKVSELIARL
                     RHAQPGVSLISPPPHHDIYSIEDLAQLIYDLKRINPRAPVGVKLVSQMGVGTVAAGVA
                     KAYADYIVIAGHNGGTGASPLSSIKHAGNPWELGLSEAQQMLRLNGLRGRVRLRTDGG
                     LSTARDVLIAALLGADEFSFGTAVLVALGCDMARQCHLNTCPTGIATQKPELRAKFRG
                     KPEHVVRFFMNIAEDLQQRLADLGLPSLQAAIGRTDLLEQVRHDGKLDLRPMLAQVGE
                     GPVSWQGKRNDRPEEHPPIDDAWVRPALSAAAAGKPFEFSVTVRNEDRSLGARLSGEL
                     ALLRAQQPDLYPDIHFRMRGTAGQSFGAFAGRGMKLVLEGQANDFVGKGLSGGELVIR
                     TRGKLARAESAQVIVGNVALYGATSGSLFAVGSAGERFAIRNSGATAVVEGVGDHACE
                     YMTGGAVLVLGPTGTNFGAGMTGGLAFVWDPSGSFVKDQKFHPEFVAVEILGACEERD
                     QDLVRSLLAEHSIKTGSKVANRLHHGWPVTLRQIVRVAPKSF"
     gene            complement(3958..4935)
                     /locus_tag="VFC15_12600"
     CDS             complement(3958..4935)
                     /locus_tag="VFC15_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11039.1"
                     /translation="MKVRVFYHDKCFDGACSAALFSRFYRERIREHAEFEYVGLVHRA
                     GALFNEAEFIGDENAIVDFKYSPSPRITWWFDHHQSAFLTPDDASHFEQDTSNTKFYD
                     PDFRSCTKFIATVAKQRFGFDPDPVAELVEWADIIDGALYPDPQTAVEMKAPAMKLTM
                     VIESNQDAGFIPRLIEMLAYEPLAEIISAPEIAGRIPGLMERHAKSIEILRELTQCKD
                     GTVFFDVTDHELEGYNKFVPYYLNPKCIYSVGLSKSSFRTKVSVGSNPWAEVEPTVNL
                     AKICERYGGGGHARVGAISFPPGKFEEARRAATEIVAELRASYLAQVAK"
     gene            complement(5104..5544)
                     /locus_tag="VFC15_12605"
     CDS             complement(5104..5544)
                     /locus_tag="VFC15_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11040.1"
                     /translation="MSATTSHAIGEERHSVSPPVRAASGGHSGDALPIPENIAGVVAY
                     LTILPAVVFLFIEPFRRNLFVRFHAFQHLYLWVAGFVLAIVAGILGMLLQLIPFMRVL
                     VFPLAGLITLAWFFLWVLLVVKAYHHQMFKLPVIGDLAEQQAGV"
     gene            complement(5736..8663)
                     /gene="secA"
                     /locus_tag="VFC15_12610"
     CDS             complement(5736..8663)
                     /gene="secA"
                     /locus_tag="VFC15_12610"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZM11041.1"
                     /translation="MINKLMAKVFGTSNEREIKRLMPLVERINALEPETKQLTEEQLR
                     AKTDEFRKRIHERVDPIEDVDEKAAALKEVLDELLPEAFAVAREASWRVLKMRHFDVQ
                     LIGGVVLHQGKISEMKTGEGKTLVATLPVYLNALAGNGVHVVTVNDYLAKRDSEWMGK
                     LYTFLGLTVGVIVHDLDDRERKEAYAADVTYGTNNEFGFDYLRDNMKFDLNDCVQRGH
                     NYGIVDEVDSILIDEARTPLIISGASEESTDKYYKVNRIIPQLELGEEIDRGAGEDKV
                     LTGDYVVDEKHRTITVTDTGWEKVERLLGIGNIADPENWDLKHHVDTAIKAHALYRRD
                     VEYVVKDGEVLIVDEFTGRLMPGRRWSDGLHQAVEAKENVKIERENQTLATVTFQNYF
                     RMYKKLAGMTGTAETEAAEFDKIYKLEVVVIPTNRDLRRVENSDVVYRTEKEKYFAAA
                     DEIMRLAQTGQPVLVGTTSVEKSERLSDLLKKKGIKHVVLNAKHHEKEAEIVAQAGRK
                     GAITISTNMAGRGTDILLGGNSEFMAKQECLKKGLAQPLKVVAGEVTAKPDDPNLSYF
                     YFSGNEYQVPTAQWQELNARFKAETDNEHEEVIKLGGLFIFGTERHEARRIDNQLRGR
                     AGRQGDPGASRFYLSLEDDLMRIFAKEWVSNLLQRLGMEEGVPIESGLITRRIEAAQK
                     AVEGQNFEARKHVLEYDDVMNKQRQAVYGLRKQLLEGVDQRELILEDYVREILGGLLD
                     QYCGKDVHPEDWDIKSLKNQIFTRFGVDVEKEGVETDKVNRQELGDALFDKLKERYDA
                     KEQLIGPEAMRHHERIIMLSVLDQQWKDHLLSMDHLREGIGLRSYGQHDPLVEYKRES
                     FDMFEAMMERFEEETVRYLYLMQVIEAPSRPAPQPVIDGNGNGDALAAVSTARRQRMA
                     TTIDDMEAEFQRRKRRELDQARMAGGDAGGSVQQVVRGDKVGRNDPCPCGSGKKYKKC
                     CGA"
     gene            8999..11755
                     /locus_tag="VFC15_12615"
     CDS             8999..11755
                     /locus_tag="VFC15_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM11042.1"
                     /translation="MFYDRFAESAQRYPDLIAVELQHESSAIASGAPAVESYSFAQLR
                     TMAESVGRWLLESGIKHGSKCAFLGANHPWWVAAYLGVLASGNTAVPLDTAFHVDQVQ
                     KLLLDCDAVLLFCDRAHLALSREAVSGLPIRIALIDGDASTAHPPSLSAIFAAGPGDF
                     HPVTVRPEDIATILYTSGTTSDPKGVMLSHANLAAEIKCVFAVIHVGPEDAILGVLPL
                     FHALAQMANLLLPFAVGARVVFLEALNTTDLLRALGERDITLFCCVPQFFYLIDVRIQ
                     KEVAQRGSIARLAFRAMLRISAISRKLGLNLGKLFFARVHKLLGPRMRYLITGGSRFD
                     ASVGQRFEDMGFTLLQAFGMTETTGGASVTPPDDVVMGSIGKPLPGNEMKVVDGVPSP
                     EHGGASVGELAFRGDIVMAGYYKRPEATAEILRDGWLFTGDLGCCDSEGNYFITGRKK
                     DVIVLSSGKNIYPEEIEAHYLQSPNIKEICVLGLQSAPGEPFSERLHAVIVPDFDALR
                     RAKIVNIGDILRFEIEGLSAKVPSTKRILSYEIWQNDLPRTTTRKIKRFEVEQRVKRG
                     EGTQAATAAPRELTPEQTEWMHQPAVSKAIGIIQSASKLKKDTPHPNDNLELDLGLDS
                     MERVELLVSLDQQMGASVPDSVASEIYTVRDLVDAVIAHSGGTSQHANTGWEDVINDP
                     PGETERATIDRDHPITAPIYYLGLRLIYLMLRDLFKLRVEGVDKLPRSGPFILSPNHQ
                     SFLDGPVMIACLPWNILRETFYVGTSEIFGSKLMRRIGEAIRLIPIDPDANLVSAMRA
                     GAYGLRQKRVLVLFPEGERSIDGPPKIFKKGAAILAIHTQCPVVPVALEGFFEAWPRG
                     EKFRGLSKLKVRFLEPVYPPALGSNPEAQYAEMTAEIRRRVVEAYYELRGTETPEPLA
                     TSTV"
     gene            complement(11785..12702)
                     /locus_tag="VFC15_12620"
     CDS             complement(11785..12702)
                     /locus_tag="VFC15_12620"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZM11043.1"
                     /translation="MTKPGATTVEKAVAKFIRALHERNASPHTIKAYTTDLAQFAGYV
                     GPQGWDDIDHMLIRGYLSSLYERGLSKTSVARALAALRSLYRWLAQEGVVDQNPAALV
                     ATPKLPKKLPRVPTIEEVNTVLDSKMQECSAFNQRDLAILELLYGCGIRNSELIGINV
                     DDIRWSNEVVLVRGKGRKERYVPLGESANAAVRAYLPLRQKILAETKRMAERALLINL
                     RGSRLTTRSVGRIVKQIAVANGLSPDVHPHTLRHAFGTHMLEEGADLRAIQEMLGHER
                     LSTTQRYTQLTVKHVMEVYDRTHPHANKS"
     gene            complement(12699..13649)
                     /locus_tag="VFC15_12625"
     CDS             complement(12699..13649)
                     /locus_tag="VFC15_12625"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HZM11044.1"
                     /translation="MQSTDPNVRLLNSFLDYLKVEKGLAALSVSAYHSDLEQYSEFLL
                     KRKRVPLAARRQDVRDFLNALLSNGVDGRSVARKLSALRHFYKYLLLDRYIKSDPTLN
                     IDSPQQWKVLPKSLAADEVESVLRGPVRVKDDHMSQALALRDRAILEVFYAGALRVSE
                     VINVKLEDLKLELGYVLVRGKGDKERIVPLGRAAQEIVQQYLHDARVVLAAGKSSPIL
                     FIARGAKRITRQRVWQIVDASSVQSGRHASPHMLRHSCATHMVENGADLRTVQTILGH
                     ADISTTQVYTHLALDRLNRVYKEHHPRSKSRLTKGSEISE"
     gene            complement(13698..14447)
                     /locus_tag="VFC15_12630"
     CDS             complement(13698..14447)
                     /locus_tag="VFC15_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZM11045.1"
                     /translation="MAKKTKNAEPSLYVGTSGWAYPIWKPEFYPKDVPAKSFLKFYAT
                     KLNAVEVNYTFRRLLSEKAAESWMSDAGPDFRFVAKANQYITHMRRLKNAEESLQRFL
                     GSLEPLLRRKQLGPILFQLPPNLKADVSVLSEFLALLPPVVQCAFEFRHESWFNDETY
                     QVLRKHNVAMCIAETESITTPEKRTAKYIYFRFRKPSYDAAEIRKLADRVERCLADGL
                     ETYAFFKHEEDPRSPLNAVELLTGVRKKLAG"
     gene            14673..16199
                     /locus_tag="VFC15_12635"
     CDS             14673..16199
                     /locus_tag="VFC15_12635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM11046.1"
                     /translation="MKLRFTALATLVLLAVMTLPLAAQDLASFEKRVTVKTLPNGLTV
                     LILRRPEAPVFSFFTLVDAGSAQDPLSQTGLAHMMEHMAFKGTPDIGTTNYAEEKIAL
                     AKVEQTYAAYEAERVKRVGRDPQKLEQLKKEWRDAIAAADKFVIKNQFGEIVESHGGV
                     GMNAGTSYDDTSYMYSMPSNMIELWAAIESDRFANPVMREFYKERNVVMEERRMRTDS
                     NPTGRLIEQFLGTAFAANPYHRPTVGYASDLQSFSATDALEFFHRYYESSNMVIALVG
                     DLDPAQVMPIVEKYFAQLPAKPKPEEVTTVEPPQNSVREVKMRDQAQPIYVEGYHRPS
                     YLDPDDAVYDAITDIMSNGRTSRMFRSLVRDQKIAAAAAGFSGFPGTKYAHLFAFYGV
                     PLPGHTNQEMQKAIQEQIAKLKSQDVTDDELQMFKTRTKADLIRGLASNEGLAQQLAI
                     YQTRYGDWRELFRYLDKVDKVTKTDIRRVANKAFQDNNRTIGSIETVMDPAAAGRGTN
                     "
     gene            16199..17683
                     /locus_tag="VFC15_12640"
     CDS             16199..17683
                     /locus_tag="VFC15_12640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM11047.1"
                     /translation="MRTQKSLFVQILFSFVLLAAGLGTAQPIPWNKLTFPPLPAFHPQ
                     QPTRIQLANGMVIFLQPDHELPLISVTARIRGGSISEPASKVGLVGLYGEVWRTGGSK
                     TKTGDQMDDFLEARAAKVETGDGSDSTTISLNCLKADFEPVFDMYLDLLRNPEFRGDK
                     LELAKQQMYTGIARRNDEVSSIVGRVSADLAYGKDNPYARDEEYATVFAVQREDLLSW
                     HQQYVYPNNIILGISGDFDPTTMEAKLRQTFDSWPKGPPTKDPDIKFTPPKPGYYLVQ
                     KTDVNQSTVSMLDLGILRNNPDYFAVSVMNEIFGGGFSSRLFNNVRSAKGLAYSVGGG
                     VGTSFSHPGMTNIEMQTKSDTTVVGIQALYEEIDKMQQRAPDVDELKRAKDQILNSFI
                     FRFDSPGKVLREKMAYEFYHYPLDFLEHYRSEIDKVTADQVTQVARKYIQKEKLSVLV
                     VGNDAEFDKPLSGLGPVTKVDISIPPPPKALMEQMGQQGPGGQN"
     gene            17819..18823
                     /locus_tag="VFC15_12645"
     CDS             17819..18823
                     /locus_tag="VFC15_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM11048.1"
                     /translation="MHPSLLQIEPPKSPAPLVGIAKLAARGESLKLGHDVEYFTLPAK
                     ALLNRCVSKRVPFDWTINPYRGCEFACKYCYARYTHEFMEMRDGLDFERRIYVKQQTA
                     WLLRQELRKVKRGEQIALGTATDPYQPAERRFEVTRGILQELALHHGLEIGIVTKSDL
                     VVRDIDMMLQIAEHNALTVSLTITTLNTKLARILEPRAPRPDLRLQALARLFAAGLKA
                     GVICAPVLPGITDSPAALDKLVRATRDAGGKYISANTLFLKPCSASIFLPFLEKEFPN
                     LVADYRKRYADRAFVSKAYSKRLSDLMASLRKKHGIVHESRHRAYPPPQELEQMNLFA
                     "
     gene            complement(18860..20269)
                     /gene="hpnE"
                     /locus_tag="VFC15_12650"
     CDS             complement(18860..20269)
                     /gene="hpnE"
                     /locus_tag="VFC15_12650"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZM11049.1"
                     /translation="MSSPSYNQKPTIAVIGGGLAGISAGSALANAGYHVELFERRPYL
                     GGRASSYELPGTGEVVDNCQHVLLGCCTNLIDFYRRLGVENQIRWYDEITFLLPDGSA
                     STLKPSWLPAPMHSGPSFLASSVLDLKDKLAISRALVALMPGLPADNGENFQSWLMRH
                     GQTKQSIDRFWAPVLISALNDELDHVSVRYAALVFRESFLKSAEAGRMGLPAVPLSQL
                     YGEAASYIEARGGRVHLRAAVDSIQADDDGVRVCVGADDVRADFAILATPFNGVAKLL
                     PDTPQMKPLREQAGYFGSSPITGIHLWFDREVTPLEHAVLLERTIQWMFQKSKILNTR
                     RESRETGSYLELVVSSSKTLVDKSRNEIIDLAMRELAEFFPAVKEAKLTKATVIKEVH
                     ATFSPTPGSDAYRPSHTSPWPRLLLAGDWTATGWPSTMEGAVRSGYGAAEALAAAGGG
                     RQTFLVPDLPPAGLMRLFG"
     gene            complement(20266..21180)
                     /locus_tag="VFC15_12655"
     CDS             complement(20266..21180)
                     /locus_tag="VFC15_12655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZM11050.1"
                     /translation="MSQHPLQLRAAYGVCRHIARSAAKNFYYGFLALPARKRNALSAV
                     YAFMRYADDISDDPSIPCELRREKLTEWMNGLRRVVDGERTDDPVLMALADTQKAFNI
                     PLDLLEKLVQGTAMDLPEADNKEKSAEGKIPATVHYETFDQLYNYCYHVASVVGLVCI
                     RVFGYRDPRAERLAEQTGVAFQLTNIIRDIKEDSELGRIYLPSEDLRRFGVDARTLTN
                     GNAVAAFRPVLEFEAQRAREYYRSAEELLPMIDDDSQPALWTLVEIYRRLLERIAARN
                     YDVFSERVQLSAAEKVGLLARGFVRRLT"
     gene            complement(21177..22148)
                     /gene="hpnC"
                     /locus_tag="VFC15_12660"
     CDS             complement(21177..22148)
                     /gene="hpnC"
                     /locus_tag="VFC15_12660"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZM11051.1"
                     /translation="MSSAPTDNRVYASQPGATSPDKAAAKGWQALPPEYAIPEHGPSL
                     AEAQRYCARLARSHYENFPVVTWFLPKHLHQHFYNVYAYCRISDDLSDEVGDKQLSLV
                     LLDQWEAELGQCYAGEPRHPVFVALRETVQEFEIPQHEFSDLLTAFRQDQIVTRYPRF
                     GDLLGYCQYSANPVGHLVLYLCGYRDAERQKLSDYTCTALQLANFWQDVAEDHSKGRL
                     YLPLEDFSRFGVTEMDLAQRRATPQFLAMMKFEVERAREWFHRGLPLAKMVDRKLARD
                     IELFGRGGLTILDAIERQGYDVLNSRPVVSTRRKLWLVARAALGNLL"
     gene            complement(22172..23209)
                     /locus_tag="VFC15_12665"
     CDS             complement(22172..23209)
                     /locus_tag="VFC15_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM11052.1"
                     /translation="MTAAVLYGKEDVKIEQVPIPKLEEGEVLVKVQVALTCGTDLKVY
                     QRGYHARMIVPPALFGHELAGVIHEVGPNVKGFKKGMRVVALNSAPCGTCFYCSKHQE
                     NLCDDLLFNNGAYAEYIRIPRRIVETNLLAIPNHVTYEEAAMTEPLACVLRGLHETKM
                     EIGDSVAVIGAGPIGLMFVQVAKLTGCNVISVVKRDSQVTAARRVGADFAVQIDKEDD
                     PVKAVRKLTVDNRGCDVVIEAVGRPQAWEWSIDMVRKGGTVNFFGGCANGTKVALDTQ
                     RLHYNELTLKATFHHTPETVRKAFALIAEKKIKGTDYITGEAPLSRLQHVLRHMLNRN
                     GDIKTAIIPGQ"
     gene            complement(23223..24344)
                     /locus_tag="VFC15_12670"
     CDS             complement(23223..24344)
                     /locus_tag="VFC15_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HZM11053.1"
                     /translation="MSIATQVNGELGGRTMPSIMRAAVYRGVNDVRLEEVPVPAIGAG
                     ELLVRVHTCGICGTDLKKIATGSHSAPRIFGHETTGVIVAVGQGVSEYQVGERVMVFH
                     HIPCGDCYYCKHKVFAQCPIYKKVGATAGFEPSGGGFAEYVRVMDWIVSRGGVVRIPE
                     GVSFEQASFIEPVNTCLKGVETLRLVQGETVLVIGQGPIGIILSVLARRCGARVVTAD
                     LFPERLTIAKTFGLNENIDASISDTVPSIREMTEGRGADVVILAVAGNGLIKPAMDAT
                     RPGGRILLFAQTQHGEAIIDPAGVCVDEKSLLGSYSASVDLQEESVRFVLGREMNLEQ
                     LISHRFSLTESIDALRLAAHPKPDSMKVVIQPGSLWKDS"
     gene            complement(24360..25064)
                     /locus_tag="VFC15_12675"
     CDS             complement(24360..25064)
                     /locus_tag="VFC15_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11054.1"
                     /translation="MPHKSVAVVVAMRRELGPLLRGVRGRREESLEFFELGSAVVLVG
                     GIGRIAALRAAQAVVARYSPTMLVSAGIAGALTATLKVGDIVRGSEVVDADSGARFMA
                     SGGESVMVTVSSVSGPAEKRTLADRYKADVVDMESAAVAAVAREHGIEFSAIKAISDE
                     LDFDMPPMARFIDGNGKFATARFATYVAIRPKWWSTVRKLRADSNRASVNLSHAVKHL
                     IEVHVNSTLESKLPRK"
     gene            complement(25054..26043)
                     /gene="hpnA"
                     /locus_tag="VFC15_12680"
     CDS             complement(25054..26043)
                     /gene="hpnA"
                     /locus_tag="VFC15_12680"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZM11055.1"
                     /translation="MKCFVTGATGFLGSHVARVMLSRGADVRLLVRPSSRTENIADLP
                     AERVVGDLRDLESLKRGMAGCEVVLHVAADYRLWAVHGQELYQSNVEGTRNVLRAARD
                     SGVRRVVYTSSVATMGFGNNGHMTDENAPVGLSNMIGDYKRSKFMAEQLVIEAARGGQ
                     DVVMVNPTTPIGERDIKPTPTGQIVVDFLKRKFPAYVDTGLNLVDVKDCAEGHLLALE
                     KAKPGERYILGAENLTLKQILDILAAITGLPSPTIRLPYAVAYATGVIDTLVTGKMLK
                     REPRVTLDSVRMGRKKMFVTSAKAERELGWNPGPVEGALQRAVEWFRAHGYAP"
     gene            complement(26099..28096)
                     /gene="shc"
                     /locus_tag="VFC15_12685"
     CDS             complement(26099..28096)
                     /gene="shc"
                     /locus_tag="VFC15_12685"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZM11056.1"
                     /translation="MAEALPPNPGEMRFGKLDDIKARVAAAIDASRKYMFSLQHPEEG
                     YWCGELGADTTLESDYVLLHRLLGTENPERTQKCANEILSHQNEDGGWPIFNGGPSNI
                     SASVKAYFALKMAGHSHEHPALAKARERILVMGGVTEVNTFTKIYLCFLGQYDYDAVP
                     AIPPEITLFPNWFWFNIYEISSWSRAILVPLSIAYAKKPFKKIPDEEGIEELFVGGRG
                     KQNLRLKWDKKLVSWRNFFLLLDRLVHFFEKVHIRPLRSIALKKAERWMLERLEMSDG
                     LGAIYPGIMNSIIALRCLGYSLDDPQVIRAMDEFERLGIEEETTFRMQPCMSPVWDTA
                     LAVFALADSGVSPTDHRLVLPSEWMLKKQVTRKGDWCVKNPKAEPAGWYFEFNNEFYP
                     DVDDTAMVMLALGKVKTSNENYQDKSVQRALDWVLSMQCKNGGWASFDKDNDRMVFQY
                     VPFADHNAMLDPATVDITGRVLESLAAYGYSMKDEPVRRALEFIKREQEPDGSWFGRW
                     GVNYIYGTMLVLRGLEAVGMDHHEPCVQQAAEWLRMMQNPDGGWGETCGSYDDPNTKG
                     VGPSTASQTAWAVMGLLAANDTRSDSLQLGIGYLLKTQLRDGSWSEAWYTGTGFPRVF
                     YLMYHMYRQYFPLIALTAYAKASAASQEEQLRITRTDRRTS"
     gene            complement(28111..29082)
                     /locus_tag="VFC15_12690"
     CDS             complement(28111..29082)
                     /locus_tag="VFC15_12690"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZM11057.1"
                     /translation="MTQTDSKTLLLLKPRGFCAGVVRAIDIVRIALETFGAPIYVRKE
                     IVHNRYVVDELTAKGAIFVDSVEEVPAGERVIYSAHGVAPEVREASKLRELRVIDATC
                     PLVTKVHVEAVKFAREGYSLVLIGHHDHDEVIGTLGEAPAVTQVVDEPEDIDNLHVPD
                     PNRVAYLTQTTLSLDETTHVIEALRKKFPNIQGPHAQDICYATENRQTAVKDVANECD
                     LLLVVGSDNSSNSNRLVEVARNMGTNSHLIENYRSIRPEWLEGARTVALTAGASAPEI
                     LVQEAVTFLQEQGFTNLREIEVMPENVRFGLPPEIVEAIGAAPALAE"
     gene            29281..29775
                     /locus_tag="VFC15_12695"
     CDS             29281..29775
                     /locus_tag="VFC15_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM11058.1"
                     /translation="MTGSELAQIYEFSYGAIQRNLDSLSHDDSMISPEPAGNCINWIL
                     GHMVTGRGLVLMLAGAAPAVLTDEEAAGYRRGSAALSAGGNAVDLARLKSAFEETQQR
                     LIPALQALSDATLSAEVPEQFRRPPLMGTVGQALVRLGYHEGYHNGQIGILRRMAGKE
                     SAIK"
     gene            complement(29779..31479)
                     /gene="recN"
                     /locus_tag="VFC15_12700"
     CDS             complement(29779..31479)
                     /gene="recN"
                     /locus_tag="VFC15_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZM11059.1"
                     /translation="MLLELRVENYAVIDNVAVEFAPGLNLLTGETGAGKSILIDALAL
                     LLGDKASAEAVRHGTEKAVVSAVFEAESKALDEVLEANGIDAGEPQLILRREISAGGK
                     GRVFVNNQPATVTVLRQLAPHLAVIHAQNEAVLAFDAASRLALLDSFAGSESAPVAEA
                     FGAWNGVRNRIAELERDEQDRLRLLDLWSFQRKEIADANLHSEEDTKLEAEKRVLANS
                     ERILSSAMGAYDALYEGNVSASALIRSAAKQLEELSRFDEKFREPLAQLDSARITVED
                     IGQSLRDYAEDIEASPERLAEVEDRLAAIDRLKRKYGPTLEGVIALGEELDRKLNEME
                     NKDEVLRRLRIELAKAAEQYLEGARKLSRHRYEAARKLEKQVESEINELAMKALFKVE
                     VSGADEEGNWTANGFDAVQYLISANPGEPLGAVEEIASGGELSRVMLALKTTIDAAVS
                     KRVRHNGAKGGPGQRTLVFDEIDSGIGGRAAEAVGKKLKQLAATKQVLCVTHLPQIAS
                     FADQHYLIEKKETGGRTKTSVRLLDTADRTRELARMISGAKLTDTSLAHAEQMLKTNR
                     "
     gene            31762..32478
                     /locus_tag="VFC15_12705"
     CDS             31762..32478
                     /locus_tag="VFC15_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZM11060.1"
                     /translation="MNVYCSLLGFVIGGEISSLIETSGLVAKTVLVILLIFSLVSWAI
                     IFSKWGMFRRARAQTNRFIRMFRKSERLQDVAAVAEQFKPSPLIAVFDGAYSELRKQA
                     PNPIRVPSLQRAIQIASSEELTRLESRLPWLATTGAVTPFIGLFGTVWGIIDAFHGLG
                     TAGAATLRAVAPGVSEALITTAAGLFAAIPAVIAYNMFTQHIREFASRMDDFGLELIN
                     EVERAQTKVTRGVPENVERR"
     gene            32478..32906
                     /locus_tag="VFC15_12710"
     CDS             32478..32906
                     /locus_tag="VFC15_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZM11061.1"
                     /translation="MAFTNNQGRTQSSLSDINVTPLVDVVLVLLIIFMVTAPVLQSGI
                     EVAVPHTKTVKEITEERLVITIDRQQKVFLGNDPININQIGARLRQKIRDPEGQSVFL
                     RADEDVPFGAFATVMDAVKQAGITNVSIVTQPLEKNGSQR"
     gene            32890..33681
                     /locus_tag="VFC15_12715"
     CDS             32890..33681
                     /locus_tag="VFC15_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZM11062.1"
                     /translation="MAANADIFPHDHVSRLSLVGSVGLHLALFALAIFNIIPSGRGEN
                     WGGTNGGGGAMSATLVTSIPLPKAQPEAQNVLANESKGLSQSKPKETPKEEPKAIPIP
                     ARDSKLKGQHEAATPKKEPPKQVAKAEPDNQVPYGQGGPVSGPYGSFTSGATKGGFGF
                     QGGQGDFGSRFAYYVDIVRRKVSDNWLKYEVDPRISDARRVYIYFEVNRSGQPTNIRV
                     EQSSGIPSLDQSAVRALQRIDTFGPLPAGYSGNYVAVEFWFDYRR"
     gene            33738..>34165
                     /locus_tag="VFC15_12720"
     CDS             33738..>34165
                     /locus_tag="VFC15_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11063.1"
                     /translation="MKRLATLILSLFFASAAFAAPPQDWIRTGTGLGVEKIRLAVPDF
                     KPAATDADSKKLAATFSTTLWNDLQAAGIFDMVSASFYPLNVPGAPQDVKLAEWQSPP
                     PNANMLAFGNAGVQGGSLAVQGWLFDVKNAQSPQVLGKQYR"
BASE COUNT         6903 a        10848 c         9320 g         7094 t
ORIGIN      
        1 gacgcccacg aaatcgagcg ccgcctttac ttcgcccgca aggcattcga acgtggcgga
       61 acggacacct acgtctgctc gctctccacc cgcacgctgg tgtacaaggc gctttgcacc
      121 ggacgcacac tgcacgagtt ctatcccgac ctgcagaaca agcagtacgc cacccacttc
      181 tcggtcttcc accagcgcta tgcgacgaac gtgttgccgt catgggaccg cgcccagccg
      241 ctgcgcatgg tggcccacaa cggcgaaatc aataccatct ggagcaatcg cgcgcacgtc
      301 gatgcccgct gcgccacgct gccgagcgaa tgcgatccga tatacacccc cgacgcttct
      361 gactcgctga gcctcgacga agtagccgag atgctctcca attatgagcg caacgtcgcc
      421 gaggcggtgc gcatgatgct gccgcccgcc gatggtccga aggaatcggc ctttcaccgt
      481 tatcacgccg actgcatgga gccatgggat ggcccatccg cactggtctt cacggatgga
      541 cgccttctgg gtgccgcact cgaccgcaac ggacttcgtc cctgccgctt cgtgatcacc
      601 gacacgggac ttgtcgtagc cggatccgaa gtcgggcttg ccgatgtgga tagcgacacc
      661 atcattcgca gcggtcgtct cggtcctggc cagatgatcc tgctcgacct ggaacggtgc
      721 cggctgatcg aaggatctga gctcgaccgc tacttcaacg atcttgctcc ttatgcaaag
      781 ttgatcgatg acattcctct ggagccttcg ggcaccccgg tttcggcgct cgacaaagac
      841 gatctcaccc gcctgcagcg cggcttcggc tacacccgcg aagaggtgaa aatggtcgtc
      901 actcccatgg cgatggagag caaagaagcc atctggtcca tgggagacga cactcccatc
      961 gcgcccctgg cgcggactcc gcggccgctc tacggattct tccggcaacg ttttgcgcag
     1021 gtcaccaatc cgccgattga ttcgctgcgc gaagcctgcg tggtttcgtc gcgcactcga
     1081 cttggaccat ggccgcatct gctcgacaag cacgcgcccc tgccgggact cgtattgccc
     1141 accccatttc tatcgctggc gcagatggcc gcgctgcacg aaggtaccta tgcgctgcac
     1201 aacgagcttc cgctcgccca tttgaactgc acgtttgccg acgacagttc actgcagcag
     1261 gcgctcatgg aactgcagaa acacgccgtc gagttggttc gcggcggcgt ttgcattctt
     1321 ctgatgaccg accgaactgc ctgcgagcac ctgttgccta tacccatggc gatggctact
     1381 ggcgcggtgc atcaagctct ggtcaaggcg ggcctacgca ccaaggttgg cctcgctctc
     1441 gaagccggcg actgccgcga cgtgcatcat gcggctgtgc tgatcggcta cggtgcgggc
     1501 tcggtgtgtc cgtggctggc cctgcagacc gctcgcacac tggccggtga ggagggcgaa
     1561 acccgcctgc tgaatgccct caactcggga ttggcgaaag taatgtcgaa gatgggcatt
     1621 tcggtcctcg acagttatcg ggctgcgcaa ctgtttgacg ttctcggcct cgattccgga
     1681 ataaccgatc tgtgcttccc cggcactgtt tctccgatag gtgggcagaa tttcgcggac
     1741 atcgaattgg cgatccgctc ttcgtggata gcggaacccg aaaccaccga acttcccgat
     1801 tacggatggg ttcgcttccg ccgctccgac cgctcggaac ctcacacttg gcaaccacag
     1861 cgcactcgcg atttgcaggt cgctgtcgga gcagctcgcg cctcgacgca agctgccact
     1921 acgatcgccc ccgcggaagc gtggtcagcc tactcgcgcg cagtggatga acgcgaacct
     1981 gccgtattgc gcgatctttt gcagatcaag gctgcagggc cgaagctgga gctgcacgaa
     2041 gtcgagccgc cgtccagcct agtaaagcgc ttcatcgcca gcgccatgtc gctgggctcg
     2101 ctcagtcccg aggcccacca gaccatcacc atcgcgatga atcagatggg cgcgcgctcc
     2161 aacactggcg agggcggcga agatcccgta gcgtataagc ccgacggaaa cacttcaggc
     2221 gttctgtcgt tgctcaacaa caagatcaag caagttgcat ccggaagatt tggcgttacg
     2281 gcggaatacc tggctcacgc cgaggaactg gaaatcaaga ttgcgcaggg ctccaagccc
     2341 ggcgaaggcg gccagcttcc cggacacaag gtcagcgaac tgattgcccg cttgcggcac
     2401 gcgcagcctg gtgtctcgct gatcagtcca ccgccgcatc acgacatcta cagcatcgag
     2461 gacctggcgc aattgatcta cgacctcaag cgcatcaacc cgcgggcgcc cgtgggcgtc
     2521 aagctggtgt cgcaaatggg agtgggaaca gtcgccgccg gcgttgccaa ggcctatgca
     2581 gattacatcg tcatcgccgg gcacaacggc ggaactggcg cctctccgct cagtagcatc
     2641 aagcacgctg gaaatccgtg ggagcttggc ctgagcgaag cgcaacagat gttgcgcctg
     2701 aacgggctgc gcggccgggt gcgcttgcgt accgatggag ggttgtccac ggcgcgcgat
     2761 gtcctgatcg cagcgctgct gggcgccgat gagttctcgt ttggcacggc ggtcctggtc
     2821 gcgctgggat gcgacatggc gcggcagtgt catctcaaca cgtgtcctac cgggattgcg
     2881 acgcaaaagc ccgagttgcg cgcaaagttc cgcggcaagc cggagcatgt cgtgcgcttc
     2941 ttcatgaata tcgcggaaga tctgcaacag aggctcgccg acctcggact gcccagcttg
     3001 caggcagcga tcgggcgcac cgatttgctc gaacaggtcc gccacgacgg caagctggat
     3061 ttgcggccga tgttggccca ggttggagaa ggcccggtta gctggcaagg gaaacgcaat
     3121 gatcgtcccg aagaacatcc gcctatcgac gacgcatggg ttcggcctgc tctctccgca
     3181 gccgccgcgg gaaagccctt cgaattcagc gtgacggtgc gtaacgaaga tcgcagcctg
     3241 ggcgcgcgac tctccggcga attggcgctg ctgcgcgcgc aacaaccgga tctgtacccc
     3301 gacattcact tccgcatgcg cggcaccgca ggccaatcct ttggcgcgtt tgccggacgc
     3361 ggaatgaagc tggtgctcga aggtcaggcg aatgacttcg ttggcaaggg cctctcggga
     3421 ggcgagttgg tcattcgcac tcgcggcaaa ctagcacgcg cggaatcggc acaggtcatc
     3481 gtcggaaacg tcgcgttata cggagccact agcggatcgc tcttcgcggt tggcagtgcg
     3541 ggcgaacgat tcgcgattcg caactcaggc gcgacagcag tggtcgaagg cgttggcgat
     3601 catgcttgcg aatacatgac cggaggcgcg gtgcttgttc tagggccgac gggaacaaac
     3661 tttggtgcgg gtatgacagg cggactcgcg ttcgtctggg accccagcgg cagcttcgtc
     3721 aaggaccaga aattccatcc cgagtttgtt gcggtggaga ttctgggtgc gtgcgaggaa
     3781 cgcgatcagg acctggttcg atccttgtta gcggaacatt ccatcaagac tggcagcaaa
     3841 gtagcgaacc ggttgcatca cggctggccg gtaaccttgc gccagatcgt gcgtgtcgct
     3901 ccgaagagtt tctgattggc tacgcaatcg tagcggctac gggccgcgca attgctctta
     3961 tttcgcgacc tgcgcgaggt agctcgctcg cagctccgca acaatctcag tcgccgcccg
     4021 ccgcgcttcc tcgaactttc ccggtggaaa cgagatggcg ccgacgcgag cgtgtccacc
     4081 tcccccgtag cgttcgcaaa tcttggcaag gttcaccgtc ggttcaacct ccgcccacgg
     4141 attcgagccg accgacactt tcgtccggaa actgctcttg ctcaacccaa cgctgtaaat
     4201 gcacttggga ttcaggtagt acggcacaaa cttgttgtaa ccctcgagtt catgatcggt
     4261 gacgtcgaaa aacaccgtgc cgtccttgca ctgggtgagt tcccgcagga tttcgatgga
     4321 cttggcgtgc cgctccatga gacccggaat acgccctgca atctcgggcg cactgatgat
     4381 ctcggcgagg ggctcgtatg cgagcatctc tatcagcctt gggatgaacc cagcgtcctg
     4441 gttcgactcg atcaccatcg tgagcttcat ggccggcgcc ttcatctcta ccgcagtttg
     4501 cggatcggga tacagggcgc cgtcaataat gtcggcccac tccaccagtt ctgccaccgg
     4561 atcaggatcg aacccaaagc gctgcttcgc cacggtggcg atgaacttcg tgcatgacct
     4621 gaagtcggga tcgtagaact tcgtattgct ggtgtcctgc tcgaaatgtg aggcgtcgtc
     4681 tggtgtcagg aatgcgctct gatggtgatc gaaccaccac gtgatacgcg gcgatggcga
     4741 atacttgaaa tcgacgatgg cgttttcgtc accgatgaat tcggcttcat tgaacagcgc
     4801 cccggcgcgg tgcaccaggc cgacatactc gaattcggcg tgctcgcgaa tgcgttccct
     4861 ataaaatcgc gagaaaagtg ccgccgaaca cgctccgtca aaacacttgt cgtggtaaaa
     4921 gacccttacc ttcacagacc ctttccgaca ccttcggcct ccggtgcgcc ggattgtgcg
     4981 tcacaaatct gaatcactca ggatacagat atccggcaat gaacggtaaa ttgaaatcac
     5041 attttgtatt tggccggtca ggcgggaatc atggattgaa atgacctggc gtgcgactgc
     5101 ggatcagaca ccagcctgtt gctctgccag gtcgccaatg accggcagct tgaacatctg
     5161 gtgatggtag gccttgacca ccagcagcac ccacaagaaa aaccacgcca acgtgatcag
     5221 gccagccagc gggaacacca gcactcgcat gaaaggaatc aactgcaaca gcataccgag
     5281 tatccccgcc acaatcgcca acacgaatcc agcgacccac agatagaggt gttggaaagc
     5341 atgaaagcgc acgaaaaggt tgcgcctgaa tggctcgatg aataagaaaa cgactgccgg
     5401 aaggatggta aggtaggcca caactccggc gatgttctca ggaatcggca gtgcgtcacc
     5461 ggagtgaccg ccgctcgcag cacgcacagg cggtgacaca gaatgccttt cctcgccgat
     5521 cgcgtgtgag gttgtcgccg acatttgctc tgcgccaggt tgaaagaccg cagtgccgca
     5581 acgcgggcag aacagtgcct gctcggacaa ggatgcacca cattgcgaac atggggacat
     5641 gggagccttt ggaaaatcga cggccatccg cctggctaaa aagcatatcc cgccgcaccc
     5701 gcgacgggat agaaagaaat cagcacgtgc agccgctacg ctccgcagca tttcttatac
     5761 ttcttgccgc tgccgcaggg gcaaggatcg ttgcggccga ctttatcgcc acggacgacc
     5821 tgttgcaccg aacctcccgc atcgccaccg gccatgcgcg cctggtccag ctcgcggcgc
     5881 ttgcggcgct ggaactcggc ttccatatcg tcgatcgtgg tcgccatgcg ctggcgtctg
     5941 gcggtactca cggcagccag ggcgtcgccg ttcccattgc catcgatcac gggttgtggc
     6001 gcaggacgcg atggcgcctc aatcacctgc atcaggtaca ggtagcgcac ggtttcttcc
     6061 tcgaaacgtt ccatcatcgc ttcgaacatg tcgaacgact cgcgcttgta ctccaccagc
     6121 ggatcgtgct ggccgtagct gcgcagtccg atgccttcgc gcagatggtc catgctgagc
     6181 aggtggtctt tccattgctg gtcgagcacg ctcagcatga tgatgcgctc gtgatgacgc
     6241 atcgcttcgg gaccgatcaa ctgctccttg gcgtcgtagc gttctttcag cttgtcgaaa
     6301 agcgcatcgc ccaattcctg gcgattgacc ttatcggtct cgacgccctc tttctcgaca
     6361 tccaccccaa agcgggtgaa gatctggttc ttcaggctct tgatgtccca gtcttccgga
     6421 tgaacgtctt tcccgcagta ctggtcgagc aagccgccga gaatctcgcg aacgtaatcc
     6481 tcaaggatga gttccctctg gtcgacgcct tccagcagtt gcttgcgaag tccgtaaact
     6541 gcctggcgct gcttgttcat cacgtcgtcg tattcgagga cgtgttttcg ggcttcgaag
     6601 ttctgtccct caaccgcttt ttgtgccgct tcaatgcgcc gcgtgatgag ccccgactcg
     6661 atcgggacgc cctcttccat tcccagccgt tgcagcaggt tcgacaccca ctccttggcg
     6721 aagattcgca tcaggtcgtc ttccaacgac agatagaatc gcgacgcacc cgggtcaccc
     6781 tggcgtccag cgcggcctcg caactggttg tcgatgcgac gcgcttcatg acgctcggtg
     6841 ccaaagatga acaacccgcc cagcttgatt acctcttcat gctcgttgtc agtctcggct
     6901 ttgaagcgag cattcaattc ctgccattgc gcggtcggga cctggtattc attgccgctg
     6961 aaataaaagt aggacaagtt gggatcgtcg ggtttcgcgg tgacctcgcc cgcaacgact
     7021 ttcaagggct gcgccaaacc cttcttcagg cactcctgtt tggccatgaa ttcggaattg
     7081 ccgcccagca gaatatcggt accgcggccc gccatgttgg tcgaaatggt gatcgcgcct
     7141 ttgcgtccgg cctgcgccac gatctcggct tctttctcgt ggtgtttggc gttgagcacg
     7201 acatgcttga tgcccttctt cttcagaagg tctgacagcc gttccgattt ttctaccgag
     7261 gtggtaccca ccagcaccgg ctgcccagtt tgcgccaggc gcatgatttc atccgccgcc
     7321 gcgaagtact tctccttctc agtgcgatac accacgtcgg agttctcgac gcggcgcaaa
     7381 tcgcggttcg tcggaatgac cacgacttcc agcttgtaaa tcttgtcgaa ctcggcggct
     7441 tccgtttcgg ctgtaccggt catgccggca agcttcttgt acatgcggaa ataattctgg
     7501 aaggtcactg tggcgagcgt ctgattttcg cgctcgatct tcacgttttc cttggcctct
     7561 acggcctggt gcagcccgtc agaccagcgg cgtcccggca tcaagcgccc cgtgaactca
     7621 tccacgataa gtacttcgcc gtctttcacg acgtactcca cgtcgcggcg atatagtgca
     7681 tgggccttga tcgcggtatc gacgtggtgc ttcaagtccc agttctcggg atcggcaatg
     7741 ttgccgatac ccagtaaccg ttcgaccttc tcccatcccg tgtcggtgac ggtgatggta
     7801 cggtgctttt cgtcgacgac gtaatcgccg gtcagcacct tgtcttcgcc tgcgccccgg
     7861 tcaatctcct cgccgagttc cagttgcggg atgatgcgat tcaccttgta gtacttgtcc
     7921 gtcgactcct cgctcgcgcc actgatgatc aacggagtac gcgcttcgtc gatcaggatc
     7981 gaatccactt cgtcgacgat cccgtagttg tgtccgcgct gcacgcaatc gttcaggtcg
     8041 aacttcatgt tgtcgcgcag gtagtcgaat ccgaactcat tgttggttcc gtaggtgacg
     8101 tcggcagcgt acgcttcttt ccgctcgcga tcgtcaaggt cgtgcacaat tacgcccacc
     8161 gttaatccca ggaacgtgta cagcttgccc atccactcgg aatcgcgctt ggcgagatag
     8221 tcattgaccg tgaccacatg gacgccattc ccggccagcg cattcaggta caccggaagc
     8281 gtggcgacca gcgttttgcc ttcgccagtc ttcatctctg agattttgcc ctggtgcaga
     8341 accacgccgc cgatgagttg cacatcgaag tgccgcatct taagcacgcg ccaactggcc
     8401 tcacgcgcta ccgcaaaagc ctcggggaga agttcgtcga ggacctcttt caacgcggcc
     8461 gctttttcgt caacgtcttc gatcgggtcc acgcgctcgt gaatgcgttt acgaaactcg
     8521 tctgtcttcg cccgaagttg ttcttcggtg agttgcttcg tctccggctc aagcgcgttg
     8581 atgcgctcga ccagcggcat gaggcgcttg atttcgcgct cattgctggt gccaaaaacc
     8641 ttcgccatca gcttgttaat caatgcagtt tttccttgcg gagcatcggt tgggggtgat
     8701 gtaagcaggc cgaagcgcgc caatttcgca cactccaacc cactaccaag cctatcacat
     8761 tggatgcaga ccgacgggca tggtcgcact ttgcaagtgc acgaaaagac ggatgttagt
     8821 accacacaac cgccgccaga ctcgcgctcg accagcctgc accgagtccg tcgcccgtcg
     8881 ggaatccgca tggaatccgg cctcgtcaat cgcttcctgc gctagaggta gtcgtgttga
     8941 cggacactgt taagatacgc gacagcattt atttccatag gattggattg tccaggcgat
     9001 gttttacgac cgctttgcgg aatcggcaca acgttatcca gacctgattg cggttgagtt
     9061 gcagcatgag tcgtcggcca tcgcctccgg agctcctgcg gtcgagagtt actccttcgc
     9121 gcagttgcgc accatggccg agtcggtggg acgatggctg ctcgaatccg gcatcaagca
     9181 cggctccaag tgcgcttttc ttggcgccaa ccatccctgg tgggttgccg cataccttgg
     9241 tgtgctcgct tccggcaaca cggccgtccc gctcgacacc gcctttcacg ttgatcaggt
     9301 ccagaagctg ctgctcgatt gcgatgccgt tctgctgttc tgcgaccgcg cgcatcttgc
     9361 cttgtctcgc gaggccgtct ccggactgcc gataaggatc gcactcattg acggcgatgc
     9421 atcgactgcc caccccccgt cgctctccgc catctttgct gccgggcccg gcgatttcca
     9481 tcccgtcaca gtcaggcctg aagacatcgc cacgattctc tacacctcgg gaacgaccag
     9541 cgatcccaag ggagtgatgc tctcccacgc caatctggca gccgaaatca agtgcgtctt
     9601 cgcggtaatt catgttggtc ctgaggacgc cattctggga gtgctgccgc tctttcacgc
     9661 gctggcgcag atggctaacc tgctgctgcc cttcgccgtc ggggcccggg ttgtgtttct
     9721 cgaagcgctc aacaccaccg atctcttgcg cgcgctcggg gagcgcgaca tcactctgtt
     9781 ttgctgcgtg ccgcagttct tctacctgat tgacgtgcgc atccagaagg aagtcgcgca
     9841 gcgcggttcg atagcacggt tggcgtttcg cgcgatgctg cggatttctg caattagccg
     9901 aaagctcggg ctgaacctcg gcaagctctt cttcgctcgt gtccacaagc ttctcggccc
     9961 tcgcatgcgc tacctcatta ccggaggatc tcgcttcgac gcctccgtgg ggcaaaggtt
    10021 cgaggacatg ggattcacgc tcctgcaggc gttcggtatg accgagacca caggcggcgc
    10081 cagcgtcacg ccacccgacg acgtcgtgat ggggtccatc ggcaagccgc ttccgggaaa
    10141 tgaaatgaag gtcgtggacg gagttccgtc gcccgagcat ggcggtgcca gcgtcggaga
    10201 actcgcgttt cgcggtgaca tcgtgatggc cggctactac aagcgtcccg aggcgactgc
    10261 ggaaattctc cgcgatggct ggctgtttac cggcgatctt ggctgttgcg attccgaggg
    10321 aaactatttc atcactggtc gcaagaaaga cgtcatcgtc ctcagttccg gcaagaacat
    10381 ttatccagaa gagatcgaag ctcactacct gcaatccccc aacatcaaag agatctgcgt
    10441 acttggtctg caaagtgccc cgggcgagcc attttctgag cgcctgcatg ccgtgatcgt
    10501 tccggacttc gacgcgctgc gccgggcgaa aatcgtcaac attggcgaca tattgcgctt
    10561 cgagatcgaa ggcctctcgg cgaaagtgcc ctccaccaaa cgcatcctga gttacgaaat
    10621 ctggcaaaac gatctgccgc gcacgacgac tcgcaaaatc aagcgcttcg aagtcgaaca
    10681 acgagtgaaa cgaggcgagg gtacgcaggc cgcgactgcc gcaccccggg agctgactcc
    10741 cgaacagacg gaatggatgc accagcctgc ggtcagcaaa gccataggca tcattcagtc
    10801 cgcctccaag ctgaagaagg acacgcctca tcccaatgac aatctcgaat tggatctcgg
    10861 acttgattcg atggagcgcg tcgagttgct ggtgtcgctc gatcagcaga tgggcgccag
    10921 cgtgcccgat tctgttgcct ccgagatcta caccgtgcgc gacctcgtcg atgccgtgat
    10981 cgcccactcc ggcggtacga gccaacacgc caacaccgga tgggaggatg taatcaacga
    11041 cccgccgggt gagaccgagc gcgccaccat cgatcgtgat caccccatca cagcaccgat
    11101 ctattatctt ggactcaggc tcatctacct tatgcttcgc gacttgttta agttgcgagt
    11161 cgagggcgta gacaagcttc cgcgctccgg cccattcatc ctctcgccca atcatcagag
    11221 ttttctcgac ggacctgtaa tgatcgcttg cctgccatgg aatatcctgc gcgagacatt
    11281 ctatgtcggc accagcgaga ttttcggtag caaactaatg cgccgtatcg gggaggcaat
    11341 ccgcctcatc cccatcgatc ccgacgccaa cctcgtgtcg gccatgcgcg ccggagccta
    11401 cggtctgcgc cagaaacgcg tactcgttct cttccccgaa ggtgagcgca gcatcgacgg
    11461 gccgcccaag atattcaaga aaggtgcggc catactagcg attcataccc agtgtccggt
    11521 cgtgccggta gccttggaag ggttcttcga ggcgtggccg cgcggcgaga aattccgcgg
    11581 cttgtccaag ttgaaggtgc gattcctcga gcccgtatat ccgcctgcgc taggttctaa
    11641 tcccgaagca cagtacgcgg agatgactgc cgaaattcgc cgaagagtgg tcgaggcgta
    11701 ctacgagttg cggggcaccg aaactccaga accgctggca acttccaccg tctgaactaa
    11761 aaactgggaa ctggaaacca attttcagct cttgttcgcg tgcggatgcg tcctgtcgta
    11821 cacctccatc acgtgcttga ccgtaagctg cgtgtagcgc tgggtcgtag atagccgctc
    11881 gtgcccaagc atctcctgga tcgcgcgcaa atcagcgcct tcctccagca tgtgtgtacc
    11941 gaacgcgtgg cgcagcgtgt gaggatgcac atcgggtgaa agcccattgg ccactgcaat
    12001 ctgcttgacg atgcggccca cgctacgggt ggtgagccgg ctgcctcgca ggtttatcaa
    12061 cagcgcacgc tccgccatgc gcttcgtctc cgccagtatt ttttgacgca gcggcaagta
    12121 tgccctcacc gctgcgttgg ccgattctcc gagtgggaca tagcgttcct tgcgcccttt
    12181 gccgcgtacc agcacgactt cattcgacca gcgaatgtca tcgacgttga tgccgatcaa
    12241 ctccgaattg cggatcccac agccatacag cagttcgagg atcgccaaat cgcgctggtt
    12301 gaacgccgaa cactcctgca tcttggagtc gagtactgtg ttcacttcct cgatcgtggg
    12361 cacgcgcggc agcttcttcg gcaacttagg tgtcgctacg agtgccgcgg gattctgatc
    12421 caccacgcct tcctgcgcca accaccgata cagcgagcgc aacgccgcca gcgcccgcgc
    12481 caccgaagtc ttgctgagtc cgcgttcgta caaactcgac aggtatccac gaatcagcat
    12541 gtggtcgata tcgtcccatc cctgcggccc aacatacccc gcgaactgcg ccaaatcggt
    12601 ggtataggcc ttgatggtat gcggcgaagc gttgcgctcg tgcagcgcgc gaatgaactt
    12661 cgctacagcc ttctcgacgg tggttgcgcc cggcttcgtc attcgctgat ctcgctgccc
    12721 ttcgtcaaac ggctcttgct gcgcggatgg tgctccttgt acacgcgatt gagccggtcg
    12781 agcgcgagat gcgtgtacac ctgcgtggtc gaaatgtcgg catgtcccag gatggtctgc
    12841 accgtacgca ggtcggcgcc attctccacc atgtgggtgg cgcaactgtg acgcagcatg
    12901 tgcggactgg cgtgacgtcc cgactgcacc gacgaagcat cgacaatctg ccacactcgc
    12961 tggcgcgtga tgcgctttgc tcctcgcgca atgaacaaga tcggcgatga cttccctgcc
    13021 gccagcacta cgcgggcatc gtgcagatac tgctgcacga tttcctgcgc cgcgcgtccc
    13081 agcggaacga tgcgctcctt gtcacccttg ccccgcacca gcacgtagcc gagttctagt
    13141 ttgaggtctt cgagtttgac gttgataact tcggacactc gcagcgctcc ggcgtagaac
    13201 acttcgagaa tcgcacggtc gcgaagggcc agcgcctgcg acatgtggtc gtctttgacg
    13261 cgaaccggac cacgcaagac gctctcaact tcgtcggccg ccagcgactt cggcagcact
    13321 ttccattgct gtgggctgtc gatgttcagt gtgggatcgg acttgatgta gcggtcgagc
    13381 agcaggtact tataaaaatg ccgcagtgcc gaaagtttgc gtgccacact tcgaccatcc
    13441 actccattcg acagcagcgc attcagaaag tcgcgcacat cttgccgacg agccgccagc
    13501 ggcacacgct ttcgcttcag cagaaactcg gaatactgtt cgagatcgga gtgataggcc
    13561 gagacactga gtgccgccaa tcccttctca actttcagat agtcgagaaa cgagttcaac
    13621 agccgcacat tgggatcggt actctgcaca aggtgagtat ggcttgcatc gagtgttgcg
    13681 ggaataacaa gcgagattca gcctgccagc ttcttccgca ctcctgtcag caattcgaca
    13741 gcattcaatg gactccgcgg gtcttcctcg tgcttgaaga acgcgtaggt ctccagccca
    13801 tccgcaagac aacgttccac acgatcggcc agcttgcgga tttcggcggc atcgtagctt
    13861 ggcttgcgga agcgaaagta aatgtacttc gcggtccgct tctcgggagt tgtaatcgac
    13921 tccgtctcgg cgatacacat cgcgacatta tgcttcctca atacctgata ggtttcatcg
    13981 ttgaaccacg actcgtgccg aaactcaaag gcgcattgca ccaccggcgg cagcagggcg
    14041 aggaattcgc ttagcaccga tacgtcggcc ttgaggttgg gcgggagctg gaagagtatc
    14101 ggaccgagct gtttgcgcct tagcaacggc tccagcgaac cgagaaatcg ttgcaacgat
    14161 tcctcagcgt tcttcagccg ccgcatatgt gtgatgtact ggttggcctt cgccacgaac
    14221 cggaagtctg gtccggcgtc gctcatccaa gactccgcag ccttttccga cagcagccgc
    14281 cgaaaggtgt aattcacttc gacggcgttc aacttggttg cgtaaaactt caggaagctc
    14341 ttcgccggaa catccttggg atagaactcg ggcttccaga tcgggtacgc ccagccggag
    14401 gttccgacgt agagagaagg ctcagcattt ttcgtctttt tcgccatata ccaatcctgc
    14461 gcttcgcctt gagagtatgc tgccgctccg acgctatgcg caaatctgct caatggtcag
    14521 gctcggcaga atgatgcccc gaccctggtt tgtacttcaa acttcaattc caccccagac
    14581 ctcccgcttt cctccgtttg acttaaccgt cttatactgc gtaactttca gagttgtagc
    14641 atcttaacgt cttcctacta tctgggggca cgatgaagct ccgtttcacc gcgctggcga
    14701 cactggtgtt gctggcggta atgaccttgc cgctggccgc acaagacctc gcgtcatttg
    14761 aaaaaagagt caccgtgaag accctgccca acggactgac agtcctgatt ctccgtcgcc
    14821 ccgaggcgcc ggtcttctcg ttcttcacgc ttgtggatgc cggctcggcc caggatccac
    14881 tctcccaaac cggcctggct cacatgatgg agcacatggc cttcaagggc acgcccgaca
    14941 tcggcaccac gaactacgcc gaggagaaga tcgctctcgc caaggttgag cagacctacg
    15001 ctgcgtacga ggcggaacgc gtgaaacgcg tggggcgcga tccccagaag ctcgaacagc
    15061 tgaagaaaga gtggcgggac gccatcgccg ccgccgacaa gtttgtcatc aagaaccagt
    15121 ttggcgagat tgtcgagagc cacggcggcg tgggcatgaa tgccggaacc agttacgacg
    15181 acaccagcta catgtactcc atgccgtcga acatgattga gctctgggcc gccattgaat
    15241 ccgaccgctt cgccaacccc gttatgcgcg agttctacaa agaacgcaac gtggtgatgg
    15301 aagagcgccg catgcgcacc gacagcaatc cgaccggacg cctgatcgag cagtttctcg
    15361 gcaccgcttt cgccgccaac ccttaccacc gtccaacagt cggctatgcc tccgacttgc
    15421 agtcgttctc ggccaccgat gcgctggaat tcttccaccg gtattacgag tccagcaaca
    15481 tggtcatcgc gctggtgggc gatctcgatc ccgctcaggt gatgccgatc gtggagaagt
    15541 acttcgcgca gttgccggcg aaacccaagc ctgaagaggt gaccaccgtc gaaccgccgc
    15601 agaactcggt acgagaggtc aagatgcgcg atcaggctca gcccatctac gtagagggct
    15661 accatcgtcc cagctatctc gatcccgacg acgcggttta cgacgcgatc accgacatta
    15721 tgtcgaatgg ccgcacttcg cgcatgttcc gctcgctagt ccgcgaccag aagatagctg
    15781 ccgccgctgc cgggttctcc ggctttcccg gcaccaagta tgcccacctg tttgcgtttt
    15841 atggcgtccc acttcccgga cacaccaatc aggaaatgca gaaagcaatt caggagcaga
    15901 tcgccaagct gaagtcgcag gatgtcaccg acgacgagct gcaaatgttc aagacgcgca
    15961 ccaaggctga cctaatccgc ggattggcta gtaatgaggg attagcgcag cagctcgcca
    16021 tctaccagac acgctacggc gactggcgcg aactcttccg ctacctcgac aaggtggaca
    16081 aggtcaccaa aaccgatatt cgtcgcgtgg ccaacaaggc cttccaggac aacaaccgca
    16141 caataggatc catcgaaaca gtgatggatc ccgcagcggc gggaaggggg acaaactaat
    16201 gcgaactcag aaatcgctct tcgtgcagat attgttctct ttcgtgctac tggcagcggg
    16261 cctcggtacc gcgcagccta ttccttggaa caaacttacc tttccgccgc ttcccgcatt
    16321 ccatccgcag caaccgacgc gtattcagct tgcgaacggc atggtgatct tcctgcagcc
    16381 cgatcacgag cttccgctga tctccgtgac cgcgcgcatt cgcggcggct cgatctccga
    16441 gcctgccagc aaagtcggac tcgttgggct gtacggcgaa gtctggcgca ccggcggtag
    16501 caagaccaag accggcgatc agatggacga cttcctcgag gcccgtgccg ccaaagttga
    16561 aaccggcgac ggctccgact cgaccacgat ctccctcaac tgtctgaagg cggacttcga
    16621 gcccgttttc gacatgtacc tcgatctgct gcgcaatccc gagttccgcg gcgacaagct
    16681 cgaactcgcc aagcagcaga tgtacaccgg catcgcccgc cgcaatgacg aagtcagctc
    16741 aatcgtggga cgcgtgagcg ccgacctcgc ctatggtaag gacaaccctt acgcgcgcga
    16801 tgaagagtac gccaccgttt tcgccgtgca gcgcgaagac ctgctgagtt ggcaccagca
    16861 atatgtttat cccaataaca tcatcctcgg tatcagcggc gacttcgatc ccacgaccat
    16921 ggaagccaaa ctgcgccaga cgttcgactc ctggcctaaa ggtccgccga ctaaagaccc
    16981 cgacatcaag ttcacgccgc ccaagcccgg ctactacttg gtgcagaaga ccgatgtcaa
    17041 ccagagcaca gtctcgatgc tcgacctcgg gattctccgc aataaccccg actacttcgc
    17101 tgtctcggtg atgaacgaga tttttggcgg tggcttctcc tcccggctct ttaacaacgt
    17161 acgctccgct aagggcctcg cctacagcgt cggaggaggt gtaggaacga gtttcagtca
    17221 tcctggcatg actaacattg aaatgcagac caagagcgac accaccgtgg ttggtattca
    17281 ggcgctgtat gaggagatcg acaaaatgca gcagagagcc ccggacgtcg acgagttgaa
    17341 gcgtgccaaa gaccagattc tcaactcgtt catcttccgc ttcgactcgc cgggaaaagt
    17401 cctgcgcgag aagatggcct acgagttcta tcactatccg ctggactttc tcgaacacta
    17461 tcgcagcgag atcgacaagg taacggcgga ccaagtgacg caggttgcgc gcaagtacat
    17521 ccaaaaggaa aagttgtctg tgctcgtggt cggcaacgat gcggaattcg acaagccgct
    17581 cagcggcctt gggcccgtga ctaaggtgga catcagtatc ccgccacctc cgaaggctct
    17641 tatggaacag atgggacaac agggcccagg tggacaaaac taactccgca gtgtggttcc
    17701 accgcgcggc ggcttcggtc gccgcttttt cttggtctga aaaacgttgc aagttgacgc
    17761 cggaccgttt tcgaggtact atccataggc gaataaaaag cgaaagtcgg tgtctgttat
    17821 gcatccctcg ctcttgcaaa tcgaaccacc caaatcccct gccccgctgg tcggcatcgc
    17881 caaactcgct gctcggggag agtccctcaa gcttggccat gatgtcgaat atttcaccct
    17941 gccggcgaag gccttgctca atcgctgcgt aagcaagcgt gtgccctttg attggacgat
    18001 caatccttat cgcggctgcg agttcgcttg caaatactgc tatgcccgct acacccacga
    18061 attcatggag atgcgcgatg gcctcgactt tgagcgcagg atctacgtca agcagcaaac
    18121 ggcgtggtta ttgcggcagg agcttcgcaa ggtcaagcgc ggcgagcaga tcgcactcgg
    18181 aacggccact gatccttatc agcctgcgga aagacgcttc gaagtcacgc gaggtattct
    18241 gcaggaactc gctctgcatc acggtctcga gatcggcatt gtcaccaagt ccgacctcgt
    18301 cgttcgcgac atcgacatga tgttgcagat tgccgaacac aacgcactca ccgtcagtct
    18361 cactatcacg acgcttaaca ccaaactcgc gcgcatcttg gagccgcgcg cgcctcggcc
    18421 cgatctgagg ctccaggcac tggcccggct gttcgcagcg ggcttgaaag ctggcgttat
    18481 ttgcgccccc gtcctgccag gtatcactga ctcccctgcg gcactcgaca aactggtacg
    18541 agccaccaga gacgccggcg gtaagtacat ctccgccaac acactgttct tgaaaccgtg
    18601 ctcggcgagc atatttctgc cgttcctcga gaaggagttc cccaatctag tcgccgacta
    18661 tcgcaagcgc tatgccgacc gggctttcgt aagcaaggct tatagcaagc ggctctctga
    18721 tttgatggcc agcctgcgca agaagcacgg aatcgtccac gaaagccggc atcgtgcgta
    18781 tcctccgccg caagagctgg aacagatgaa tctgttcgct tgattcggag atcgagagac
    18841 tggcggacgc tagttgcaat tatccgaata gtcgcattag ccctgccggg ggaagatccg
    18901 gcacgagaaa cgtttgacgg ccgccacccg ccgccgccaa ggcctcggcg gcgccgtatc
    18961 cactgcgcac cgcgccctcc atggtggaag gccagcccgt cgccgtccag tctccggcga
    19021 gaagaagtct cggccatgga ctggtgtgcg acggacgata agcatcgctc cctggcgtag
    19081 gcgaaaacgt cgcgtggact tctttgatga cagtcgcctt ggtcagcttc gcctctttga
    19141 ccgcgggaaa gaactccgcc agttcccgca tggccaggtc aatgatttcg tttcgcgatt
    19201 tatcgaccag cgtcttagac gagctgacaa ccagttcgag atagcttccg gtttcgcgcg
    19261 actcgcgccg ggtgttcagt atcttcgact tctggaacat ccactggatc gtccgctcca
    19321 gaagtaccgc gtgttcaaga ggcgtgacct cgcgatcgaa ccacaggtgg atacccgtaa
    19381 tcggcgacga accgaaatag ccagcctgtt cccgcagtgg cttcatctgg ggcgtatcgg
    19441 gcagcagctt agcaacaccg ttgaacggcg tggccaggat ggcgaagtca gcgcgcacat
    19501 cgtcggcgcc gacgcacaca cgaacaccat cgtcgtccgc ttgtatcgaa tcaaccgccg
    19561 cccgcagatg cacgcgcccg ccccgcgcct caatgtacga cgccgcctct ccatagagtt
    19621 gcgacaacgg aacggcggga agccccatgc gccccgcttc cgccgacttg aggaacgatt
    19681 cgcggaacac cagcgccgca tagcgcacgg aaacgtgatc gagttcgtca ttgagagcgc
    19741 taatcaacac cggcgcccag aagcgatcaa tcgactgttt ggtctgtccg tgtcgcatca
    19801 gccaggactg aaagttctcg ccgttgtcag caggaaggcc aggcatcagc gccaccagcg
    19861 cccgcgaaat cgccagcttg tctttcagat cgagcaccga agacgccagg aaagacggcc
    19921 ccgagtgcat gggtgcgggc aaccaactcg gcttcagggt cgaagcgcta ccgtcaggaa
    19981 gtagaaaggt gatttcgtca taccagcgga tctggttttc aactccgagg cggcgataga
    20041 aatcgatgag gttggtgcag caacccagca acacgtgctg gcagttgtcc accacttcgc
    20101 cagtccccgg aagctcgtac gacgaggcgc gtccgccgag gtagggcctt cgctcgaaca
    20161 attcaacatg gtagcccgcg ttggccagcg cgcttcctgc ggagatgccg gccaatccgc
    20221 caccgatcac cgcgattgtc ggcttctggt tgtaactcgg ggaactcatg tcagccggcg
    20281 cacaaatcct ctcgccagga gaccaacttt ttcggcggcg ctgagttgca cgcgctcgct
    20341 gaacacgtca taatttcggg ccgcaatacg ttccagcaat cggcggtaga tttcgaccag
    20401 cgtccacaag gccggttggc tgtcgtcatc gatcatcggc agcagttctt ccgctgaacg
    20461 gtagtactcc cgggcacgtt gagcttcaaa ctccaacacc ggacgaaacg ctgcgacagc
    20521 attcccgttg gtcaacgtcc tggcatccac gccaaagcgc ctcaggtctt cgctcggcaa
    20581 ataaatgcgc cccaactcac tgtcctcttt gatgtcccga ataatgttag tgagctggaa
    20641 tgccactccg gtctgctcgg caagtctttc cgcgcgcgga tcgcggtaac cgaaaacccg
    20701 aatgcagacc aaccccacta ccgaagccac gtggtagcag tagttgtaga gttgatcgaa
    20761 ggtttcgtag tgcaccgtcg caggaatctt gccctctgcg gacttttcct tattgtccgc
    20821 ttccggcaag tccatcgccg tgccctgcac cagcttttcc agaagatcga gcggaatgtt
    20881 gaacgccttc tgggtgtcgg ccagcgccat tagcacggga tcgtcggtgc gctcgccgtc
    20941 cacgactcga cgcaacccat tcatccactc cgtcagtttc tcgcggcgca attcgcacgg
    21001 gatggaggga tcgtcggaaa tgtcgtcggc gtaccgcatg aacgcgtaca ccgctgacaa
    21061 ggcattgcgc ttgcgagccg gcagcgccag gaagccataa tagaaattct tggctgcaga
    21121 gcgggcgatg tgtcggcaga ctccgtaggc ggcccgcaat tgcagcgggt gctggctcac
    21181 agcagattac ccagcgccgc tcgtgccacc agccacagct tgcgtcgcgt ggagaccacc
    21241 ggccgcgagt tcagcacatc ataaccctgt cgctcgatgg catccagaat cgtcagtccg
    21301 ccccggccga acagttcgat atcccgcgcc agctttcggt ccaccatctt tgccagcggc
    21361 agtccacggt gaaaccactc gcgcgcgcgc tccacttcga acttcatcat ggcgaggaat
    21421 tgcggagtcg cgcgccgttg cgcgaggtcc atttccgtca ctccgaaccg cgagaaatct
    21481 tccagcggaa gatagagtcg gcccttgctg tggtcctcgg ccacatcctg ccagaagttt
    21541 gccagttgca gagcggtaca ggtgtagtca gagagcttct gccgctcggc gtcgcgataa
    21601 ccgcacaggt agagcaccag gtggcccacg gggttcgccg aatactggca atatcccagc
    21661 aagtcgccga agcgcggata gcgcgtcaca atctgatcct gccggaacgc cgtgagcaaa
    21721 tccgagaatt cgtgctgggg aatctcgaat tcctgtaccg tctcgcgcag ggcgacaaat
    21781 acgggatgtc gtggctcgcc cgcatagcac tgccccagct ccgcctccca ctggtccagc
    21841 agtaccaggg acaattgctt gtcgccgacc tcgtcgctca gatcgtccga gatccggcag
    21901 taggcataga cgttatagaa gtgctgatgc agatgcttgg gcaggaacca tgtaacgacc
    21961 gggaaatttt cgtagtgcga acgcgccagc cgcgcacaat accgttgcgc ttccgcgagc
    22021 gacggcccat gctccggaat agcgtactcc ggcggcagag cttgccagcc tttagcggcg
    22081 gccttatcgg gagacgtagc gcccggctgc gacgcataca cccggttgtc agtcggtgcg
    22141 gaagacatga gttgtggtat accccggtcg cctactgtcc cggaataatt gcggtcttaa
    22201 tgtcgccgtt gcggttcagc atgtggcgca gaacatgctg caaccgcgat agcggcgcct
    22261 cgccagtgat gtagtccgta cccttgatct tcttttccgc gatcagggca aacgctttgc
    22321 ggaccgtctc tggcgtgtga tggaacgtgg ccttcagcgt gagctcgttg tagtgcaggc
    22381 gctgggtatc gagcgcgacc ttggttccat tggcacagcc gccgaagaaa ttcaccgtgc
    22441 cgcccttacg aaccatgtca atcgaccact cccatgcctg cggacgtccc acggcctcga
    22501 tcaccacgtc gcagccgcgg ttatccacgg taagcttgcg gaccgccttg accgggtcgt
    22561 cctctttgtc gatctgcacc gcaaagtcgg cgccaacgcg gcgcgcggcc gtaacctgtg
    22621 aatcgcgctt taccacggaa atcacgttgc atccggtgag cttggccacc tgcacgaaca
    22681 tcagtccaat cggaccggcg ccgatgacgg ccaccgagtc gccaatttcc atcttggttt
    22741 catgcaaccc gcgcaacacg cacgccagcg gctcagtcat ggcggcttcc tcgtaggtca
    22801 catgattcgg gatcgccagc aaattggtct caacgatgcg ccgcggaatg cgaatgtact
    22861 cggcataggc tccattattg aacagcaggt catcgcagag gttttcctgg tgcttcgaac
    22921 aataaaagca cgtgccgcac ggcgccgaat tcagcgccac cactcgcatg ccttttttga
    22981 atcctttgac gttgggccca acttcgtgga tgaccccggc caattcgtgg ccaaacagcg
    23041 ctggaggcac gatcatgcgg gcgtggtagc cccgctgata gaccttcaaa tcggtaccgc
    23101 aggtcaaggc cacctgaacc ttgaccagca cttcgccttc ctccaacttt gggatcggca
    23161 cttgctcgat tttgacgtcc tctttgccat agaggaccgc ggctgtcata tttccgttca
    23221 cgctatgaat ccttccataa gctacccggc tgaatcacca ctttcatgga gtccggtttg
    23281 ggatgggcag ccaaccgtag cgcatcaatc gactcggtca gtgaaaaacg atggctgatg
    23341 agctgctcta aattcatttc gcggcccagc acaaatcgta ctgactcctc ctgcaaatcg
    23401 accgaagcgc tgtatgaacc caatagactc ttttcatcaa cgcaaacgcc agcgggatcg
    23461 atgatggctt ccccgtgctg cgtttgcgcg aacagcaata tacgcccgcc aggacgggtt
    23521 gcatccatgg ccggcttgat caaaccgttc ccagctaccg caagaataac tacatcagcc
    23581 cctcggccct ccgtcatttc ccttatcgac gggaccgtat cggagatgga ggcgtcgatg
    23641 ttttcgttga gcccgaacgt cttagctatt gtaagcctct ccggaaacaa atcggcagtg
    23701 actactctcg caccacacct tcgggccaaa actgaaagga taatgccaat cgggccctgc
    23761 ccgattacca gcaccgtctc tccctggacc agcctcaggg tttcgacacc cttcaagcag
    23821 gtgttcaccg gttcaatgaa cgacgcctgt tcaaacgata cgccctccgg aatgcgcacg
    23881 actccgcccc ggctgacgat ccagtccatc actcgaacgt actcggcaaa cccgccgccg
    23941 ctgggctcga agcctgccgt cgctccaact ttcttgtaga tcgggcactg cgcgaagacc
    24001 ttgtgcttgc agtaataaca atcgccacat ggaatgtgat ggaacaccat cacgcgctcg
    24061 cccacctgat actccgaaac tccctgtccg acggcgacga tcacgcccgt ggtttcgtgc
    24121 ccgaaaattc gtggcgcgga gtgcgatccc gtcgcaatct tcttcaaatc ggtgccgcag
    24181 attccgcagg tgtgaactct taccagcaac tcgccggcgc caatcgcggg aactggcacc
    24241 tcttccaatc gaacatcgtt gacgccgcga tacaccgccg cgcgcatgat cgagggcata
    24301 gtccgcccac ccaattcccc gtttacttgt gtcgcaatgg acatgcaaaa cttgcccgct
    24361 tacttccggg gcaacttact ctccagcgta ctgtttacgt gtacctcgat tagatgtttc
    24421 accgcatgac tcaggttcac tgatgctcga ttgctgtccg cccttagctt gcgtacggtt
    24481 gaccaccact tcggccgaat cgccacatat gttgcgaacc gcgccgtggc gaacttgcca
    24541 ttcccatcaa taaaacgcgc catcgggggc atgtcaaagt ccaattcatc cgagatggct
    24601 ttgatcgccg aaaactcgat cccatgctcc cgtgcgacgg cggcgactgc ggccgactcc
    24661 atatcaacca catcagcctt gtagcgatca gccaatgtcc gcttctcagc cgggccgctg
    24721 actgacgaca ctgttaccat aaccgactcg ccgccactcg ccatgaatcg tgcgccagaa
    24781 tcggcatcta caacttcact accccggaca atatctccga ccttcaacgt cgcagtgagc
    24841 gcgccagcga tacccgctga aactaacatc gtggggctgt atctcgccac aaccgcctgg
    24901 gcagcgcgca aggcggcgat tcgtccgata ccgccgacca ggaccaccgc gcttccgagt
    24961 tcgaaaaact caaggctctc ctcccgccgt ccccgaacgc ctcgcagcag cggacccaat
    25021 tctcggcgca tcgcgaccac cacggccacg ctcttatggg gcatagccat gcgcccggaa
    25081 ccactccacc gcccgctgca atgccccctc taccgggccg ggattccatc ccaactcgcg
    25141 ctcggccttg gcgctggtga caaacatctt cttgcgtccc atgcgcaccg aatccagcgt
    25201 cacccgcggt tcgcgtttca acatcttgcc tgtgaccagc gtatcgatca cacccgtggc
    25261 ataggcaacg gcgtacggca accgtatggt cggcgacggc agtcccgtga tggccgcgag
    25321 tatgtcgaga atttgcttaa gcgtcaggtt ttcggctccc aggatgtacc gctctcccgg
    25381 ctttgccttc tccagcgcca gcagatgtcc ttcagcgcaa tcctttacgt ccaccaggtt
    25441 gaggcccgta tccacgtagg caggaaactt gcgcttcaga aaatccacca caatctggcc
    25501 ggtcggcgtc ggcttaatgt cccgctctcc aattggagtt gtcgggttca ccatcaccac
    25561 atcctggccg ccccgtgccg cctcgattac caactgctcc gccatgaact tgctgcgctt
    25621 gtagtcgccg atcatgttcg ataagccgac cggagcattc tcgtcggtca tgtggccgtt
    25681 gttgccgaac cccattgtgg ccacgctact ggtatagaca acccgccgca cgcccgaatc
    25741 tctcgccgcc cggagaacgt tgcgtgtgcc ctcgacgttc gactgataga gttcctgtcc
    25801 gtggacagcc cataagcgat aatcggctgc cacgtgaagc acgacttcgc aaccggccat
    25861 cccgcgcttc agtgattcca gatcgcgaag atcgccaacc acgcgctccg cagggaggtc
    25921 ggcgatattt tccgtccggc tgctgggtct caccagcagg cgcacatcag cacccctcga
    25981 gagcatcacg cgagccacgt ggcttcccag aaaccccgtc gcccctgtga cgaaacattt
    26041 catgatggta attccgcgcc ccagcggaat cgctgcgagc ctgcgcgcgg cttagcgttc
    26101 agctcgtccg gcgatcggta cgagtgatcc ggagttgctc ttcctgcgac gccgccgaag
    26161 ccttggcata cgcggtcaag gcaattagcg ggaaatactg acggtacatg tggtacatca
    26221 gataaaacac gcgcgggaat cctgtcccgg tgtaccacgc ttcactccac gatccgtcgc
    26281 gaagctgcgt cttcaacaaa tagccaatgc cgagctgcag cgaatcgctg cgcgtatcgt
    26341 tggccgccag caggcccatc accgcccagg cggtttgcga tgcggtgctc ggtcctacgc
    26401 ccttggtgtt cggatcgtcg taggagccgc aagtctcgcc ccatccgccg tccggattct
    26461 gcatcatccg cagccattcc gccgcctgct gcacgcaagg ttcatggtgg tccatgccca
    26521 ctgcttcgag tcctcgcaac accagcatgg tgccgtagat atagttcaca ccccagcggc
    26581 caaaccacga tccatccggc tcctgctctc gctttatgaa ttccagcgcg cgacgcaccg
    26641 gctcatcctt catggaatag ccataagcag ccagcgactc cagcacgcgg ccggtgatat
    26701 ccaccgtcgc cggatcgagc attgcgttgt ggtcagcaaa cggaacgtac tggaacacca
    26761 tccggtcgtt gtctttgtcg aacgaggccc atccgccgtt cttgcactgc atcgagagga
    26821 cccagtccag cgcccgttgc actgacttgt cctgatagtt ctcattggac gttttcacct
    26881 tgccgagcgc cagcatgacc atggcggtgt cgtccacgtc gggataaaac tcgttgttga
    26941 attcgaaata ccagcctgcg ggttcggcct tgggattctt tacgcaccag tcgcccttgc
    27001 gtgtcacctg cttcttcagc atccactccg aaggcagtac caggcgatgg tccgtcgggg
    27061 aaactccgct gtcggccagc gcaaaaaccg cgagagccgt gtcccacact ggcgacatgc
    27121 agggctgcat tcggaaggtt gtttcttcct cgatgcccag tctctcgaat tcgtccatgg
    27181 ctcggatgac ctgcggatca tcgagcgagt agcccaggca acgcaatgcg atgatcgaat
    27241 tcatgatgcc aggatagatc gcgcccagcc cgtcactcat ctccaagcgc tccagcatcc
    27301 accgctcggc cttcttgaga gcgatggagc gtagcggccg gatgtgcacc ttctcgaaga
    27361 agtgcaccag ccgatcgagc agcaggaaaa agttccgcca gcttacgagt ttcttgtccc
    27421 acttcaagcg cagattttgc ttgccgcggc cccccacgaa tagctcttca attccctcct
    27481 cgtcaggaat cttcttgaag ggcttcttgg cataagcaat cgatagcggc accaggatgg
    27541 cccgcgacca tgacgaaatt tcgtagatgt tgaaccagaa ccagttgggg aacagcgtga
    27601 tctccggtgg aatcgccggt accgcatcat agtcatactg gccaaggaaa caaaggtaga
    27661 tcttggtaaa ggtgttgacc tcggtcactc cacccattac cagaatgcgc tcgcgtgcct
    27721 tggcaagtgc tggatgttcg tgactgtggc ccgccatctt gagcgcaaag taggccttta
    27781 ccgacgcgct gatgttcgac ggccccccat tgaagatcgg ccagccgcca tcttcattct
    27841 gatgcgacag aatctcattg gcgcacttct gcgtgcgctc cgggttctcg gttcccaaca
    27901 gccggtgcaa aagtacgtag tcggattcca gcgtggtgtc ggcgcccagt tctccgcacc
    27961 agtacccttc ctcagggtgc tgcagggaga acatgtactt gcgagacgcg tcgatcgcgg
    28021 ctgcaactcg ggccttgata tcgtccagct tcccaaatcg catttcgccg ggattagggg
    28081 gtaacgcttc tgccattctt tctcttgctc ctactccgcc agggctggcg cagccccgat
    28141 ggcttcgacg atttcaggtg gcagcccgaa acgaacattc tccggcatca cttctatttc
    28201 ccgcagattc gtgaaaccct gttcctgcag gaacgtaacc gcttcctgta ccaggatctc
    28261 cggcgcagac gcgcccgctg tcaaagcaac tgttcttgcg ccttccagcc attccggccg
    28321 gatcgatcgg tagttctcga tcaggtgcga gttggtcccc atgttgcgcg caacctcaac
    28381 caggcggttg gaattcgaac tattgtcaga cccaaccacc agcaaaaggt cgcattcgtt
    28441 ggcgacatcc ttcaccgctg tctggcggtt ttccgtcgca tagcaaatgt cctgcgcgtg
    28501 cgggccttga atatttggga atttcttgcg caacgcctcg atcacgtgag tcgtttcgtc
    28561 cagactcaac gttgtctgtg tcaggtaggc cacgcgattg ggatcgggga catgcaaatt
    28621 gtcgatatct tcgggctcat ccactacctg ggtcacagcc ggagcctcac ccagcgtccc
    28681 aattacctcg tcgtggtcgt gatgtccaat cagaaccagc gagtaacctt cgcgcgcaaa
    28741 cttcaccgcc tctacgtgga ccttcgtcac cagcgggcac gtcgcatcga tcacccgcag
    28801 ttcgcgcagt ttgctggcct cgcgaacttc cggagctact ccgtgcgcgc tgtatatcac
    28861 gcgctcaccg gccggaactt cctccacgct atcgacgaaa atcgcgcctt ttgcagtcaa
    28921 ttcgtccacc acgtaccggt tgtgcacgat ttccttgcgg acgtagatcg gcgccccaaa
    28981 agtttccaga gcaatccgga caatatcgat ggcgcgaacc accccggcgc agaagcctct
    29041 tggcttcaac aaaagcagag ttttggagtc ggtctgagtc atggattcct taatgattta
    29101 cacggatatt aacccatgta ttcccacagt tactctattg tagcagcgcc agaaccaaca
    29161 acataacgtc aagaaactgc aagatttagc agtctgcggc gcttttcccg cagttaacgg
    29221 caaccgcccc gacaagtctc tatgacccac cgagtacaat cccatggagg agccatactt
    29281 atgaccggca gcgaactcgc ccagatttac gaattcagtt acggagccat ccagcgcaac
    29341 ctcgatagtc tcagccacga tgacagcatg atcagcccgg aacccgccgg caactgcatc
    29401 aattggattc tcggccatat ggtcacgggg cgaggattgg tcctgatgct ggccggtgcg
    29461 gcacctgcag tcctcacgga cgaggaagct gcgggttaca ggcgcggttc cgcagccctc
    29521 agcgccggag gaaacgccgt cgacctcgcc cgcctgaaga gcgcgttcga ggagacccag
    29581 cagcgcctga ttccagcact tcaggctctc agcgacgcca ccctgtccgc cgaagttccc
    29641 gaacaattcc gacggccccc gttgatggga accgtaggtc aggcgttggt caggctcggc
    29701 tatcacgagg gctatcacaa cggacagatc ggcatcctgc ggcgtatggc gggaaaagaa
    29761 tccgccatca agtagcactc accggttcgt cttcaacatc tgctcggcat gtgccagcga
    29821 cgtgtcggtc agtttcgctc cgctgatcat gcgcgccagc tcccgcgtgc gatccgcggt
    29881 gtcgagtagt cgtaccgagg tcttcgttcg tccgccagtc tctttcttct cgatcagata
    29941 gtgctgatcg gcgaatgacg cgatctgcgg caggtgggta acgcacagca cctgcttggt
    30001 agcagcgagt tgtttcagct ttttacctac ggcctctgcc gcccgccccc caatgcccga
    30061 gtcaatttcg tcgaacacca gcgtccgctg cccaggtccg cccttggcgc cgttatgccg
    30121 cactcgcttc gacaccgctg catcgatggt ggtcttcagc gccagcatca cccgcgaaag
    30181 ttcgccgccc gacgcaatct cctccaccgc ccccagtggc tctcccggat tcgccgagat
    30241 aaggtactgc accgcgtcga aaccattcgc ggtccaattc ccttcctcgt ctgctccgct
    30301 gacctcgacc ttgaagagcg ctttcatcgc cagttcgttg atctcgctct cgacctgctt
    30361 ctccaacttg cgggcagcct cgtagcgatg ccgcgaaagc ttgcgcgccc cttccaaata
    30421 ctgctccgcc gctttcgcca gctcgatccg cagccgtcgc aggacttcat ccttattctc
    30481 catctcgttg agcttgcggt ctagttcctc gcccaacgcg atcacgcctt ccaaagttgg
    30541 cccgtactta cgcttcaagc ggtctatcgc tgccaaccga tcttccacct ccgccagacg
    30601 ctcaggcgat gcctcgatat cctccgcata gtcgcgtagc gactgtccga tgtcctcgac
    30661 cgtgatgcgt gccgagtcca gttgcgccag cggctcccga aacttctcgt caaacctcga
    30721 cagctcttca agttgctttg ccgctgagcg aatcagtgcc gacgccgaca cgtttccctc
    30781 gtacagcgcg tcatatgcgc ccatggcgga tgacagaatg cgttccgaat ttgccagcac
    30841 ccgcttctcg gcttccagct tggtgtcttc ctccgaatgc aagttcgcgt ccgcaatttc
    30901 tttccgttgg aagctccaca gatcgagcaa ccgcaaccgg tcctgttcat cgcgctccag
    30961 ttcggcgatg cggttgcgca cgccgttcca cgcgccgaac gcctccgcga ccggcgcaga
    31021 ttcagacccc gcgaagctgt cgagcaacgc cagtcgcgac gccgcgtcaa acgccagcac
    31081 tgcttcgttt tgcgcatgga tgaccgccag gtgcggcgcc aattgccgca gtacggttac
    31141 cgtcgccggc tgattgttga cgaagacgcg tcccttccct ccggcggata tctcgcgccg
    31201 caagatgagt tgcggttcgc ccgcgtcgat gccgttcgcc tccagcactt cgtccagcgc
    31261 tttcgactcg gcttcaaaca ccgctgatac cacagctttt tccgtgccat ggcgcaccgc
    31321 ttccgccgag gctttgtcgc ccagtaacaa tgccagcgca tcaatgagaa tggatttacc
    31381 ggcgcctgtt tcgccggtca ggaggttgag ccccggggcg aactccacgg cgacgttgtc
    31441 gatgaccgcg tagttttcta cccgcagttc cagcagcaca gtgagttctc gattgcgaag
    31501 ttatgccgca gtgtagcagg aaaactctcg gttcagcatg aggaatgcac ggaccgagtc
    31561 atgtttccac ccgcgacgcc cgagccgcgt cagcggcgac acggtcccat ctacacaatc
    31621 cgaaccgtcc gcatcgcatt tcaatcgcgt gactgacatt ggccaccgtg gttacttcgc
    31681 aggaacctcg tcatacgaac ccgccctgcc ctcgtctaaa ccttataatg cggacaatgg
    31741 aagcgcaggc caactaacgg aatgaatgtt tattgcagtc tcctcggctt tgttatcggc
    31801 ggtgaaatct ccagtctcat cgaaaccagc ggtctcgtcg ccaaaaccgt tcttgtcatc
    31861 ctgctgatat tcagccttgt ctcctgggcc atcattttct ccaagtgggg aatgttccgc
    31921 cgtgcccgtg ctcagaccaa ccgcttcatt cgaatgttcc gcaagtccga gcgcctgcag
    31981 gacgtcgccg ccgtggcgga gcagttcaag cccagcccgc tgatcgcagt atttgacggc
    32041 gcctatagcg aactccgcaa gcaggcgccg aatcccatcc gcgtgccctc gttgcagcgt
    32101 gcgattcaga ttgcttcgtc cgaagaactg acgcggctcg aaagccgtct gccctggctg
    32161 gcaacaaccg gcgcagtcac accgttcatc ggcctcttcg gaacggtgtg gggcatcatc
    32221 gacgccttcc acggactcgg caccgcggga gccgccacct tgcgcgcggt cgcgcccggc
    32281 gtctccgaag ccctcatcac caccgcggcc ggcctcttcg ccgccatccc ggccgtcatc
    32341 gcttacaaca tgtttaccca gcacatccgc gagttcgcct cccgcatgga cgacttcggc
    32401 ctcgaactca tcaacgaagt ggagcgcgcc caaaccaaag tcacgcgcgg tgttcccgag
    32461 aacgtggagc ggcggtaatg gccttcacca acaatcaggg acgcacccag tcttcgctct
    32521 ccgacatcaa cgtcacgccg ctcgtggacg tggtgctcgt gctcctcatc atcttcatgg
    32581 tgacggcgcc cgtgttgcag tccggcattg aagtggccgt gccccacacc aagacggtga
    32641 aggaaatcac cgaagagcgt ttggtgatca ccatcgaccg ccagcaaaaa gtatttctcg
    32701 gcaacgatcc catcaacatc aaccagatcg gcgcgcggct gcgccagaag attcgcgacc
    32761 ctgaaggcca gtctgtcttt ttgcgtgcgg acgaagacgt tcccttcggt gctttcgcca
    32821 ccgtgatgga cgcggtcaag caagccggca tcaccaacgt gagcatcgtc acccaaccgc
    32881 tggagaagaa tggcagccaa cgctgacatc tttccgcacg atcatgtctc ccgcctgtcg
    32941 ctggtcggct ccgtcgggct gcatctggca ttgttcgccc ttgcgatctt caacatcatc
    33001 cccagcggcc gcggcgagaa ctggggcggc accaacggag gtggaggtgc catgagtgcg
    33061 accctggtca ccagcatccc tctgcccaaa gcgcagcccg aggcgcagaa cgttttagcc
    33121 aacgagtcca agggcttgtc gcagtcgaag cccaaagaaa ctcccaagga ggagccgaag
    33181 gcaatcccga ttcccgcacg cgactccaaa ctgaagggac agcacgaggc agcgactccc
    33241 aagaaagagc ctcccaagca ggtggccaaa gctgagcctg ataaccaagt tccctacgga
    33301 caaggcggcc cggttagcgg cccttacgga tcgttcacct ccggcgccac caaaggcgga
    33361 ttcggatttc agggcggcca gggcgatttc ggcagtcgct ttgcctatta cgtggacatc
    33421 gtccggcgca aggtatccga caactggctg aagtacgaag tcgatccccg catctccgat
    33481 gcccgccgcg tctacattta tttcgaggtg aatcgttccg gccagccgac caacattcgc
    33541 gtagagcagt cgagcggaat tccctcgctc gatcagtctg cggtgcgcgc tttgcaacgc
    33601 atcgatacat tcggtccgtt gcccgcgggc tactcgggaa actacgtcgc cgtggaattc
    33661 tggttcgact accggcgctg aggcccacgc gttgtcgcag cgtgaacacc ggacattagt
    33721 ttatgcttga cttattaatg aaacgactcg caactctcat cctctcgctt ttcttcgcca
    33781 gcgcggcctt cgccgctccc cctcaagatt ggattcgcac cggtaccggc ctcggtgtgg
    33841 agaagattcg cctcgcggta cccgacttca agcccgcggc cacagatgct gattccaaga
    33901 agctcgcggc gaccttcagt acaactctgt ggaacgacct gcaggccgcc ggaatcttcg
    33961 acatggtttc agccagcttc tacccgctaa acgtcccggg cgcaccgcag gacgtcaaac
    34021 tcgccgaatg gcaatccccg cctcccaacg ccaacatgct cgccttcgga aacgccggcg
    34081 tacagggcgg atcgttggcg gtgcagggct ggctctttga cgtgaagaac gcgcaatcgc
    34141 cgcaggtact cggcaagcag taccg
//
LOCUS       DASRKK010000548         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_340562, whole genome shotgun sequence.
ACCESSION   DASRKK010000548 DASRKK010000000
VERSION     DASRKK010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340562"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFC15_12725"
     CDS             <1..242
                     /locus_tag="VFC15_12725"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZM11064.1"
                     /translation="AIVLCILQLGDLLSTRLALAHGAVEANIFVRPIGLWAAKLLCIA
                     IVGVLAWRTRKPSRIWAVCAFYVIVVGWNLSLLLR"
     gene            complement(503..718)
                     /locus_tag="VFC15_12730"
     CDS             complement(503..718)
                     /locus_tag="VFC15_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11065.1"
                     /translation="MREWCRFRDWLISYCLHQDPIALRLLRLAENSPVEFEVEASLHG
                     SSLNLNLPDWCGAMDFCDGYEAPAKKR"
     gene            complement(711..2000)
                     /locus_tag="VFC15_12735"
     CDS             complement(711..2000)
                     /locus_tag="VFC15_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM11066.1"
                     /translation="MLDRRRFLTTTAGFAGAIAFRNDLFAQLQNVPSSLPDPKLFISD
                     DEGYWAELRKQFLIPADEVYLNNGTVGSSPAPVLRAVFDGYTSTERLDETDPEDYPIW
                     GYAAWNEFRDPLAAFVGCTRDEIALLRNATEANSYIANGIDMKPGDEVLMTDQEHPGG
                     EQPWQLKAKRYGIAVKKVTLPKPVKDSAQVLNLFNDAITPRTRIIFFSHITTVTGVVL
                     PAKELCALARSKGILSAIDGAHVTGMMRVNIHDLGCDMYTSSPHKWLQAPKGSGYLYV
                     RDEVIDRLWNTIATEGWEEPKLRAERFQRIGSSNVPSLCGLRASIKMANEIGMERIER
                     RHRELGDYMLVQMQKRGAESWTSPDPALRCAIVTVNVPPIQRTDLETWLWKTRKIRIR
                     GGEPSKLRLSTPYYLQKKDLDRFLDAFDEYKREKKIA"
     gene            complement(2128..>2354)
                     /locus_tag="VFC15_12740"
     CDS             complement(2128..>2354)
                     /locus_tag="VFC15_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11067.1"
                     /translation="LFVSGTRDAFGSIAELESAIKLIPAPTKLVPIEGAGHSLLQKSN
                     REGLPETVVREFLTFFSHPFAKRARKDGAP"
BASE COUNT          459 a          687 c          682 g          526 t
ORIGIN      
        1 cagctatcgt tctctgtatc ctacaactcg gcgatctgct atcaacgcga cttgccttgg
       61 ctcatggcgc agttgaggcc aatatattcg taaggcctat cggactctgg gcggcgaagc
      121 tgctctgcat tgcgattgtc ggggtcttag cttggcgtac ccgcaagccg tcacggatat
      181 gggccgtttg tgcgttctac gtgatcgtag tgggctggaa tctttcactt ttgttgcgct
      241 gaggcatctc attcgcaatg ttcgggaaaa agggctaaga gcagtatgta gctgatgagg
      301 atggtcagat caattgcgat tttcttcgtg gtttcgaggt gagacgtact gcttgcgtct
      361 cagcggcgga atgccccgtt taatcgcggc gttcatcgac tgtcagtttt cggagcgaga
      421 attgagcgaa gccgcacctt gagcgactta atcggctgca cgcgcttctc accttttcct
      481 tctcggctcg ctcagttcat gttcaccgct tcttcgcggg ggcctcgtag ccatcgcaga
      541 agtccatcgc gccacaccag tctgggaggt tgaggttcag gctgctgccg tgcagcgatg
      601 cctcgacttc gaactccacg ggagagttct cggccaacct cagcaagcgt agggctatgg
      661 ggtcttgatg caggcaatag ctgataagcc agtcgcgaaa ccggcaccat tcacgcaatc
      721 ttcttttccc gcttatactc gtcgaaagca tcgaggaagc gatcgaggtc tttcttctgc
      781 aagtaatacg gcgtcgagag ccgcagcttc gacggttcgc cgccgcggat gcgaatcttc
      841 ctagtcttcc acagccaagt ctctagatcc gtacgctgga tcggtggaac gttcaccgta
      901 acgatggcgc aacgcagtgc cgggtcgggc gatgtccacg actccgcgcc gcgtttctgc
      961 atctgcacca gcatgtagtc gcctagctcg cgatgacgcc gctcgatacg ctccatgccg
     1021 atctcgttcg ccatcttaat ggacgcgcgc agcccacaaa gcgacggcac attggaagat
     1081 ccaatccgct ggaagcgctc ggcccgcagc ttcggttctt cccaaccttc ggttgcgata
     1141 gtgttccaca agcgatcgat cacctcgtcg cgcacgtaca gataccctga tcctttgggc
     1201 gcctgcagcc acttgtgcgg gctcgaggtg tacatgtcgc aaccgaggtc gtggatattg
     1261 acacgcatca tgccggtgac gtgagcgccg tcaatcgccg agagaatgcc cttggagcgc
     1321 gccagcgcgc agagttcctt cgccggcaac accacgccgg ttacagtggt gatgtggctg
     1381 aagaagatga tccgcgtacg cggcgtgatg gcgtcgttga acagattgag gacttgtgcc
     1441 gagtccttca ccggcttggg caacgtgact ttctttaccg cgatgccgta gcgctttgcc
     1501 ttcaactgcc acggctgttc gccgccagga tgctcctggt cggtcatcag cacttcgtcg
     1561 cccggcttca tgtcgatgcc gttggcgatg tagctgttgg cctcggtggc attacgcagc
     1621 aaggcaatct cgtcacgcgt gcagccaacg aacgcagcca gtggatcgcg aaactcattc
     1681 catgccgcgt agccccagat cggataatct tcgggatcgg tctcgtccag cctttccgtc
     1741 gaggtgtagc cgtcgaagac cgcgcgcagc accggcgcgg gactggatcc cactgtaccg
     1801 ttgttcaggt aaacctcgtc cgcgggaatc aggaattgct tgcgcaactc ggcccaatat
     1861 ccttcgtcgt cgctgatgaa cagcttggga tccggcagcg acgacggaac attctgcagt
     1921 tgcgcgaaaa gatcattgcg aaaggcgata gcgccagcga atccagcagt tgtagtgaga
     1981 aagcggcggc gatcgagcaa gagcacctcc ggcaaataaa ggcaacatgg tacacccgag
     2041 gccgatcacg ggcgaatcgc caccaacgcg tggcgtgtct catcccggcg taacctgtca
     2101 tcccggagtg gagggacctg cttttattta gggtgcccca tcctttcgcg cgcgtttcgc
     2161 gaaagggtgg gagaagaagg tgagaaattc ccgcaccacc gtctccggca gtccctcgcg
     2221 gttggacttc tgtagcaggc tatgtccggc gccctcgatt ggaaccagct tcgtgggtgc
     2281 aggaatcagc ttgatcgcac tctccagttc cgcgatcgag ccaaacgcgt cgcgcgtacc
     2341 gctgacgaag agaa
//
LOCUS       DASRKK010000549         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_438394, whole genome shotgun sequence.
ACCESSION   DASRKK010000549 DASRKK010000000
VERSION     DASRKK010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438394"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFC15_12745"
     CDS             <1..491
                     /locus_tag="VFC15_12745"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZM11068.1"
                     /translation="QLPLSLCNTICTNVPGPQVPLYLMGHKMLCWYPWVPIGGLMGIN
                     CAILTYNGTAFFGFTGDVHAAPDLKRLEKFVDESFAELRAGAGAIAEAEVGAGAKPQR
                     QKRVRPNGKIPIANAKTPVAENAASQQRRKRKPRNKKIPVALVEAPDFSPAKQATENS
                     GL"
     gene            510..1004
                     /locus_tag="VFC15_12750"
     CDS             510..1004
                     /locus_tag="VFC15_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11069.1"
                     /translation="MPLAPQEIRTFFVTGATWGRRPIFRAEPMAHLFLDTLKRYRKQG
                     RFLLHEFVLMPDHFHLLLTPAPDVSLEKALQFLKGGFSFRVKRELRSNVEIWQTGFTE
                     HRVRDWDDFQQHAIYIRENPVRAHLAENAIAYPYGSATGGFETDGEPPWLKPQLTIMR
                     GSPG"
     gene            1478..>2152
                     /locus_tag="VFC15_12755"
     CDS             1478..>2152
                     /locus_tag="VFC15_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11070.1"
                     /translation="MTRLLRHSCALAIAVVCFSTIASADLKIKTRTTVMGHTSESTVY
                     IKGARQRSEMSFGGHGGSVSIIQCDQKRMITISGNQCMVMPMGGGEGSCPATPNMGAM
                     AREMAGGESTPTPKGGVVTITRTSNDTGERQDMFGYKARHIKTSMMMESSPDACNQSH
                     MKMEMDGWYADLSAGFSCADESYRALACGGTGKRGCSDRIVMKGGGGGALGYPLKLTT
                     TIASDQG"
BASE COUNT          475 a          630 c          633 g          414 t
ORIGIN      
        1 gccaactgcc gctgagcctg tgcaatacca tctgcaccaa cgtccccggc ccgcaagtcc
       61 cgctgtacct catgggtcac aagatgctgt gctggtatcc ctgggtgccg attggcggcc
      121 tgatgggcat taattgcgcc atcctcacct ataacggcac ggcgttcttc ggcttcaccg
      181 gcgatgtgca cgcggcgccc gatctcaagc gcctggagaa gttcgtggat gagagctttg
      241 cggagttgcg ggcgggcgct ggcgccatag ccgaagccga agtcggagcc ggagcaaagc
      301 cacagcggca gaagcgcgta cggcctaatg ggaagattcc gattgcgaat gcgaagaccc
      361 cggtcgcgga aaatgctgca agccagcagc ggcgcaaacg gaaaccaaga aacaaaaaga
      421 ttccggtcgc tctggtggaa gcgccggact tcagcccggc gaaacaggct acggaaaact
      481 cggggcttta gccctggtga gtaagaacaa tgccgcttgc gccgcaagaa attcggacct
      541 tcttcgtgac gggagcgacg tggggaagac ggccaatatt tcgcgctgag ccgatggcgc
      601 acctcttctt ggacacgctc aaacggtatc gcaagcaagg acgatttctg ttgcacgaat
      661 ttgtcctcat gccagaccac tttcacctcc tgctaacgcc cgcgcccgac gtttcactgg
      721 aaaaggcgct gcagttcctc aaaggagggt tctcctttcg cgtcaaacgg gagctgcggt
      781 cgaatgtgga aatttggcag acggggttta cggaacaccg agtacgggac tgggacgact
      841 ttcagcagca tgcgatttac attcgcgaga atcccgtacg tgcgcatctt gcggagaacg
      901 cgatcgccta tccgtacggt tcagccacgg gcggatttga gaccgatggc gaacctccgt
      961 ggctaaagcc acagctgact atcatgagag gttcacctgg ctgaagaggc tacggaaaaa
     1021 ctctgtgcca aacccgtgga gtgtacttct tggtggaagc ccccgcattt atgcggggga
     1081 aggagcgctt cagcgctccg ggaaaagttc ggctttgatc acgcgcttta gcgccgggat
     1141 cgagaaatcc cggggctaaa gcccatttca aaatcgtctc cttctccgct agactaaagt
     1201 ctagctcccc ctgctaaagc agggggcttc caccaaaagg cccccacaga gttcttgagc
     1261 agcctctgaa gcccggcgct tccaccccaa agcatatcgc gcgtggctct aagggtactg
     1321 atgggcaact cgctgacggc ggctcatgga agatggaatc aatgatccat ctcgggcact
     1381 gaaatacaca cctgtcgtat tccgcacggt ttgtttagaa ttgcgccgta ctcattgcaa
     1441 cgtaaaaatg ttcgtgtttc ctttgaggag gcctctcgtg actcgcctgt tacgtcattc
     1501 ctgtgctctt gcgatcgccg tagtttgctt ttccaccatc gcctcagccg acctgaagat
     1561 caagacccgc accacggtga tgggccacac ctcggagagc acggtgtaca tcaagggggc
     1621 ccgccagcgc agcgagatga gcttcggagg tcacggcggc tctgtcagta tcatccaatg
     1681 cgaccagaag cgcatgatca ccatcagcgg caaccaatgc atggtgatgc ccatgggcgg
     1741 cggcgaaggc tcttgtcccg cgacgccgaa catgggcgcc atggcacgag agatggcagg
     1801 cggcgagtcc acacctaccc ctaagggtgg cgttgtaacc atcacccgca cctcaaacga
     1861 tactggcgag cgccaggaca tgttcggcta caaggcgcgc cacatcaaga cttcgatgat
     1921 gatggagtcc agccccgacg cctgcaatca atcccacatg aagatggaaa tggatggctg
     1981 gtacgccgat ttgtcggcgg gattttcctg cgccgacgag tcgtaccgcg ctctggcctg
     2041 cggcggcacg ggcaagcgcg gttgcagcga ccgcatcgtg atgaagggcg gcggtggcgg
     2101 cgcactgggc tatccgctta agctgaccac caccatcgcg tcagaccagg ga
//
LOCUS       DASRKK010000550         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1122586, whole genome shotgun sequence.
ACCESSION   DASRKK010000550 DASRKK010000000
VERSION     DASRKK010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122586"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..704)
                     /locus_tag="VFC15_12760"
     CDS             complement(438..704)
                     /locus_tag="VFC15_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11071.1"
                     /translation="MKSQQLLTQSKIFEDEILAGPECTNNPAEEVPEPPDHAQNLTGT
                     SPIELDAKSLILRVYDVLMNDKGTLAGKRYSDLPAARGRVASPL"
     gene            897..1637
                     /locus_tag="VFC15_12765"
     CDS             897..1637
                     /locus_tag="VFC15_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM11072.1"
                     /translation="MSKEIRALIFQMVAENPTWGAPRIHGELLKLGFDLSEPTVSRWV
                     RRAPRPPDPVRRWLTFLRNHREAIAAMDFFTVPTLTFGVLYCFFVIGHDRRRILHHSV
                     TRNPNALWVALQLHEAWEYSQQPQRFLIFDRDTKFSADVVSTVKAMGSQPIRTAFRSP
                     WQNGIAERWVGSVRRDLLNHVIVLNRKHLRRLLNEYVRYYHEDRTHLGLGKDTPGGRV
                     PASMPSNRHKVISLPRLGGLHHRYTVAA"
BASE COUNT          433 a          580 c          563 g          467 t
ORIGIN      
        1 ccccttcttt gggcccgatc ttacaggctg tcccggcggc ccgccgccac acccactacc
       61 cacttgcgcg cagtgtagga aggaaggttg cgcctgtcga gccgggggcg ggtgcttgtg
      121 tcagtgatgc gagtttgccg ctgtgcctcg tcagaactaa acgcagcacc gtttcgccta
      181 cgatagctcc ttgcgtggat cgcacctgcc ttgagtcgtg cggtcttcga cggctgtgac
      241 tactgtgcct cgccacaatc caaaacgcac caggagcagt gcatactcaa gagttcgctg
      301 atatcaggac ctaacttttc tgtggcgttg aatggccaga tgcgcgggac ttcttcgttc
      361 ccggctgaat gagcggacac tgctgtcgtt ggcacagaca aatgaaagct tgcctacagc
      421 cgattcaagc agcccggtca tagcggtgat gcaacccgcc cacgcgctgc tggcaaatca
      481 ctgtaccgtt ttccagcgag cgtgcccttg tcattcatca aaacatcgta cacccgtaat
      541 atcaatgatt tggcatcaag ctcgattggc gacgttccgg taagattctg agcatgatca
      601 ggtggctccg gcacttcctc ggctgggttg ttagtgcatt caggtcccgc gaggatctca
      661 tcctcgaaaa tcttgctctg cgtcagcagc tgctggctct tcacgcccag cgccctcgcc
      721 ggcgactccc tgcgtcacac aagctgttct gggtcgtatt gcgaaggctc tgggataaat
      781 ggaaagaacc tctcatcctg gtgacaccca gaaccgttgt cggctggcac cgttctggat
      841 tccggctgta ttggaaatgg ctttcaagag ccaagtccag gggaggacgc aagccggtga
      901 gcaaagagat tcgagcctta atctttcaaa tggttgccga aaatccaacc tggggagcgc
      961 cccgcattca tggcgagttg ctcaagctgg gcttcgatct atcggaacca acggtttcgc
     1021 gttgggtgcg gcgagccccg agacctccag atcccgtcag gcgctggctg actttcctga
     1081 ggaaccatcg cgaggcgatc gcagcgatgg attttttcac tgtgccgaca ctgaccttcg
     1141 gcgttctgta ctgcttcttc gtcatcggcc acgaccgtcg tcgaatcctt caccacagcg
     1201 tgactcgtaa tcccaacgcg ctttgggttg ccttgcagct gcacgaagct tgggaataca
     1261 gccaacagcc gcagcgattc ctgatcttcg atcgggacac caagttcagt gccgatgtgg
     1321 tctcgaccgt gaaggcgatg ggcagccaac cgattcgcac cgcattccgc agtccctggc
     1381 agaacggaat tgcggagcgc tgggttggca gtgtgcgtcg cgatctcctg aatcatgtga
     1441 tcgtgctgaa tcgaaaacat ctgagacggt tgctgaacga gtacgtccgt tattaccacg
     1501 aggacaggac tcatctcgga ttgggcaagg acactcctgg tggtcgagtt ccagcatcaa
     1561 tgccttcgaa tcgccacaag gttatctcgt tgccgcgact cgggggactg catcaccgct
     1621 ataccgtcgc ggcctaacaa tttccagctc cgaactggac gcgttcgcgg tcaggatcgg
     1681 agatagattc acgcctggat ctttgcagtc gagctctcct gctcgtttcg gacgctcata
     1741 gcggtcgaga ctacgccatg caaagtgctg ccttccaagg aaagggcctc ctgcatcgaa
     1801 gactcggcgg cgtttagttt tgatgaacga caacggcaag gctatggctg agccgatgcg
     1861 ggaagcgaat agcgaagtag tgagattggt aatccagtaa tggagtgcta acggagtgcg
     1921 aactcggtca accgcttgta actaattgaa attacaagag ttatggcgga gagacaggga
     1981 ttctaatcct ccctccgcct aaagcctttc agattcaaat actaagaccg ttaaccctta
     2041 ttc
//
LOCUS       DASRKK010000551         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1025273, whole genome shotgun sequence.
ACCESSION   DASRKK010000551 DASRKK010000000
VERSION     DASRKK010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025273"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..408
                     /locus_tag="VFC15_12770"
     CDS             124..408
                     /locus_tag="VFC15_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZM11073.1"
                     /translation="MATLSTTSLKLDSAMKERVHRLASARRRSPHWVMREAVEQYVER
                     EEKREQLRQDALAAWADYQTSGLHVTAKEADAWLAKLETGKNAVIPKCHV"
     gene            396..686
                     /locus_tag="VFC15_12775"
     CDS             396..686
                     /locus_tag="VFC15_12775"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM11074.1"
                     /translation="MPRLRWSQPALRDVARLINFLAPKSRDAAQRAVKTIRQGVKALG
                     KHPEIGRPVEELPPEFREWVIEFGQGAYVALYHYDGKQVVILAVRHGREAGY"
     gene            complement(744..2006)
                     /locus_tag="VFC15_12780"
     CDS             complement(744..2006)
                     /locus_tag="VFC15_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11075.1"
                     /translation="MHDSRIRSRLKAQLTKFSSELCERLTRPLAGFVSEMLFGIQSSQ
                     DVKLSNISRSLQEKIPLIRTEKRLSRNLKSAELEKELTPQLVRMGSSRIQPNTVLALD
                     LSDVRKEYAQKMENLATVRDGSTGELHQGYWLCDVTGAEVNGSEIVPLYQKLYSVEAK
                     DFTSENAEVLAAVDLIRTHTQGRGIWTIDRGGDRKKLLEPLLDRGERFVIRSTGKRTV
                     IDRRKLQGSVAEVAGRCRLRHQARIIKIQDGKEKVYDLRYGAEPIRLPGRAEQLWLVV
                     IDGFGEAPLMLLTNVPIGAKDSQSLWWIVQIYLTRWKIEETFRFIKQSYNLEDIRVMN
                     YQRLKNLGILVTAVAYFAATFLGQQMKLRILCEKLMIISQRFFGIPPFRFYALADGIR
                     RILSQGTFSPPETPPASLQMELTLGWEG"
BASE COUNT          416 a          625 c          606 g          438 t
ORIGIN      
        1 cttcaaatcg cggttagtat agcattccag gccacttggc cgccggggaa ggtgagggca
       61 gtgaaagagg cccatacgct gctgtgctaa agtgctacca ggtaacacct tggagcactc
      121 gcgatggcga cactttctac aaccagcctg aagctcgata gtgcgatgaa agaacgtgta
      181 catcggctcg cctcggcgcg tcgccggtcg ccgcactggg tcatgcgcga agccgtcgag
      241 cagtatgtgg aacgggagga gaagcgcgag cagctccggc aggacgcgct tgctgcatgg
      301 gccgactatc agacctccgg cctgcacgtc acggccaagg aagccgacgc ctggctggcc
      361 aagctcgaaa cgggcaaaaa cgctgttatc cctaaatgcc acgtctaagg tggtcccaac
      421 cggccctgcg ggatgtggcc cgtctcatta attttctggc tcccaagagc cgggatgccg
      481 cacagcgcgc cgtcaagacg atccggcagg gtgtgaaggc gctcggcaag cacccggaga
      541 ttggtcgtcc tgtcgaggag ctgccgccgg agtttcgcga gtgggtgatt gaattcgggc
      601 agggggccta cgtcgccctg tatcactacg acgggaaaca ggttgtgatt ctggcggtcc
      661 gccacggacg cgaagccgga tattgagcta actcctcgaa aggaaaggca acgggattac
      721 cggcaggagt ttccccagaa ttctcagccc tcccagccaa gggtcaactc catctgtagg
      781 ctggcgggtg gcgtttccgg cggactgaag gtgccttggg agagaattct tcggattcca
      841 tcggccagcg catagaaccg gaacggcgga atgccaaaga agcgctgtga gataatcatc
      901 aacttttcgc aaaggatgcg cagcttcatc tgctgcccga gaaacgtggc ggcgaagtaa
      961 gccacggcgg tcacgagtat gcctaagttc ttcaatcgct ggtagttcat cacgcggata
     1021 tcttccaggt tgtagctctg tttgatgaaa cggaaagttt cctcaatttt ccagcgcgtc
     1081 aggtagattt gcacgatcca ccaaagggat tgtgagtcct tggctccaat cggcacgttg
     1141 gtcagcaaca tcagtggcgc ctcgccgaag ccatctatca ccaccagcca gagttgctcg
     1201 gctcggcccg gcaggcgaat cggctcggcg ccgtagcgca gatcgtagac cttctctttc
     1261 ccatcctgga ttttgatgat gcgcgcctga tgacggagcc ggcaccgccc cgctacctcg
     1321 gccacgctgc cttgcagctt gcgccggtcg atcaccgtgc gcttgccggt ggagcggatg
     1381 acgaagcgct caccgcggtc cagtaggggt tccagcaact tcttgcggtc gccgccacga
     1441 tcgatggtcc agatccctcg cccctgggta tgcgtccgga tcaggtccac ggcggccagc
     1501 acttccgcgt tctcgctggt gaagtctttc gcctcgaccg agtacagttt ctgatagagg
     1561 gggacgatct cgctgccgtt gacctcggcc ccggtcacgt cgcagagcca gtagccctgg
     1621 tgcagttcgc ccgtgctgcc gtcgcgcacc gtagccagat tttccatttt ctgggcatac
     1681 tccttacgga catcgctcag gtccaacgcc agcaccgtgt tgggctgaat gcgcgagctg
     1741 cccattctta ctaactgcgg agtcagctct ttctccaatt ccgcgctctt caagttacgc
     1801 gatagacgct tctcagttcg gatcagagga atcttctcct gcagcgaacg cgagatgtta
     1861 ctcagcttca catcctgact cgactggatg ccgaacagca tctccgatac gaacccagcg
     1921 agcggccttg tcaggcgctc acaaagctcc gaggagaact tcgtcaactg cgctttgagt
     1981 ctggatcgaa ttctgctatc gtgcaccggg ggacgcctcc gtggtggttt ggatttctcg
     2041 taaaaaccct tctaccacgg atttcgtccc ctcccacttc tcaat
//
LOCUS       DASRKK010000552         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_48062, whole genome shotgun sequence.
ACCESSION   DASRKK010000552 DASRKK010000000
VERSION     DASRKK010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48062"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VFC15_12785"
     CDS             complement(<1..1659)
                     /locus_tag="VFC15_12785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM11076.1"
                     /translation="MIGKTISHYRVTEKLGGGGMGVVYKAEDTRLGRFVALKLLPEDL
                     AHDAQSLGRFRREARTASALNHPNICTVYDIDQEEGRAFIAMEFLDGVTLKQHIAGRP
                     VDSELLLTLAIEIADGLDAAHQAGIMHRDIKPANIFVSKRGHAKILDFGLAKVRFAGS
                     ALAGTADETVALSAKDLSLPGAIMGTVGYMSPEQVRGDQVDSRTDLFSFGAVLYEMAT
                     GKMAFHGESPVDICAEILRKNPAPPSELNAKVQPKLENLIQRALEKDPALRYQHATDM
                     RADLQRLKRDWDSQRSPDASRLAPAASKRLPWLLAAVLLLVAIMTGWYFVSHRPAKLT
                     DKDTIVVGDFDNKTGDSVFDDTLKQGLAVQLEQTPFLALMSDNKVNQTLKLMGRPSGD
                     RLTPEVTREVCQRTGSKAMMTGSIASLGSQYVIGLKAADCDSGDVLAEVQEQAASKET
                     VLKALGTAAVSLRGKLGESASSVRKYATPLPEATTPSLEALQAYSQGWRTFDMKGGAE
                     ALPFLNRAIELDPNFALAYSALSIVYNTLDQPGRSAEAARKAYEL"
     gene            complement(2145..>3642)
                     /locus_tag="VFC15_12790"
     CDS             complement(2145..>3642)
                     /locus_tag="VFC15_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM11077.1"
                     /translation="LQLAVRVASMGIWELQFSTRTLIWAPEVRDIFGVPANAPPPSFE
                     DAIRFTHPEDRSLVEEHMVLLLVGKSIQLDHRIIRPDGKVRWIEVTGKAEFDDTGQPV
                     RCLGFIRDVTERHQLEEQFVQAQKMEAVGRLSGGVAHDFNNMLMVITGYGDLLKEQLG
                     SDERLRPMIEAILKAANRAASLTQQLLAFSRKQILIPRVVDLNSVLADMGKMLPRLIG
                     EDIDLKIVPGNGLGRVMADASQIQQVVMNLVVNARDAMPDGGRLTIETENAEWDEEPG
                     QEYGIETKPGSFVLLSVADNGTGMDRETRAHLFEPFFTTKGVDKGTGLGLSIVYGVVK
                     QSGGFISVDSEPGQGTTFKIYLPGIEAEETNTSPTKVPPKSRRGSETILLVEDEQSVR
                     EAISHFLRGQGYTVLEAHNPRVAIEIAREQSKQIHLLMTDMIMPKLNGRELAKQLTAT
                     RPEMRVLYISGYTDRGIGGGTIDSTMNFLQKPFGFDALGRKVRDILTS"
BASE COUNT          740 a         1072 c         1021 g          809 t
ORIGIN      
        1 cagttcataa gctttgcgtg ccgcctctgc ggatcgtccc ggctggtcga gagtgttgta
       61 aacgatggat aacgcggagt aggccaaggc aaaattcgga tcaagttcga tcgcgcggtt
      121 cagaaaaggc agtgcctcag cccctccttt catgtcaaac gtcctccatc cctggctgta
      181 ggcctgcaac gcctccaggg atggggtggt cgcctcgggc aaaggcgtgg cgtacttccg
      241 caccgagctg gccgactcgc ccaacttgcc gcgcaaactc accgcggccg tgcccagcgc
      301 tttgagcacg gtctccttgc tcgccgcctg ctcctgcacc tccgccagca catctccgct
      361 gtcacaatcg gctgccttca ggccaatcac gtattgactg cccaagctcg cgatggaacc
      421 ggtcatcatg gctttgctgc cggtcctctg gcagacctcc cgcgtaacct caggcgtcag
      481 acggtcgccg gacggacggc ccataagctt caacgtttga tttactttgt tgtcggacat
      541 cagggcgaga aacggtgttt gttcgagctg caccgccaga ccctgcttga gcgtgtcgtc
      601 aaacacgctg tcaccggttt tgttatcgaa gtctccaacg acgatggtgt ctttgtcggt
      661 gagcttggcc ggacgatggg agacgaagta ccacccggtc atgattgcga ccagcagcag
      721 taccgcagcg agcagccagg gcagccgttt gctcgccgcc ggcgccagcc gcgatgcatc
      781 gggagagcgt tgcgagtccc aatcgcgctt cagtcgctgc aggtcggcac gcatgtccgt
      841 cgcatgctgg taccgcaggg caggatcttt ttccagtgcc cgctgaataa gattttccaa
      901 cttaggctgc actttcgcat tcagctccga aggaggcgcg ggattctttc gcaaaatctc
      961 cgcacagatg tcgacagggc tttcgccgtg aaacgccatc ttgcctgtgg ccatctcata
     1021 gagcacggcc ccgaacgaga acagatcggt gcgcgagtca acctggtcgc ctcgcacctg
     1081 ctccggcgac atatagccaa ccgttcccat gatggcgcct ggaagggaca ggtccttcgc
     1141 actcagtgcg actgtctcgt cggcagttcc cgccagggcc gaccctgcga acctgacctt
     1201 cgccaagccg aagtccagta tcttggcgtg tccgcgcttg ctgacgaaga tgttggccgg
     1261 cttaatgtcg cgatgcatga tgcccgcctg gtgtgcggcg tccagcccgt ctgcgatttc
     1321 aatcgccagc gtcagcaaca gctcagagtc gacagggcgg ccggcgatgt gctgcttcag
     1381 cgtcactccg tcgagaaatt ccatcgcaat gaatgcacgg ccttcctcct ggtcgatgtc
     1441 atagaccgtg catatgttgg gatggttgag ggccgaagcg gtccgcgctt cgcgccggaa
     1501 tcgcccaagc gactgcgcgt catgggcgag atcttctgga aggagcttga gggcgacgaa
     1561 gcggccaaga cgcgtatctt cggccttata cacaacgccc atcccgccgc caccgagttt
     1621 ctcggtgact cgatagtgcg agatggtttt gccgatcatg cgattaggcg ggtcctccgg
     1681 tggctcatgt taggggtact gtgaggtggc agtcaaacta ctccggcagg gagaccgatt
     1741 ggggtcgagt cgctaaagaa agattggtct cgataaatgc cccagtgtcc ggaaatccac
     1801 ttgagcgaat agtcggctat gcttcctggt gctttggatc acgctggacc gaaatcaaaa
     1861 tgacggaatg ggcgttttac gtcaaattga cgtaagtgga cttcacgagc ggcccggcga
     1921 agcttcccga acgccgttta tggacaatca agacaagcgg gggattcgct gatcattttg
     1981 tgcgggacgg atagggtgtg agatggcctg ataccaacag ctatcttgat ttgcaggaag
     2041 ggatcatggc aacgcgccgc caattcagcg agtcgcggta gcgcgcccgg aaccaacagc
     2101 acatggcgtt ggaaaggagt acgtatcgcc ggatacacct cagttcaact ggtcagaatg
     2161 tcgcggacct tgcgcccaag cgcgtcaaaa ccgaacggct tctggaggaa gttcatagtg
     2221 gagtctatcg tgccacctcc tattcctcga tcagtataac cggagatgta tagcaccctc
     2281 atttcggggc gcgtagcggt tagctgttta gccagttccc ggccattcag cttaggcatg
     2341 atcatgtcgg tcataagcaa gtgaatctgt ttactctgtt ctcgggcaat ctcgattgca
     2401 accctgggat tgtgtgcctc cagcacggtg tacccctggc cgcgaagaaa gtgagaaatc
     2461 gcctcgcgga cactctgctc atcctcaacc agcaggattg tctccgaacc acggcggctt
     2521 ttcggaggaa ctttggtcgg cgaagtattt gtttcctccg cctcgatacc tggcaggtag
     2581 attttgaacg tagtgccctg tcccggttcg ctatccactg agatgaagcc tccactctgc
     2641 ttcacgactc cgtacacaat cgagagcccc agtcctgtgc ccttatccac acccttcgtt
     2701 gtgaagaacg gctcaaagag gtgcgcccgg gtctcccgat ccatgccggt accgttgtcg
     2761 gccacggata gcaacacaaa cgagcctggc ttggtctcaa tgccatactc ctgcccaggc
     2821 tcctcgtccc attcggcgtt ttcagtttca atcgtcaatc ttccaccgtc aggcatcgcg
     2881 tcgcgagcat tgaccactag gttcataacg acctgttgaa tctgcgaggc gtcggccatc
     2941 acgcggccca gcccgttacc gggaactatt ttcagatcaa tgtcttcgcc aatgagacga
     3001 ggcaacatct tgcccatgtc agccaggacg ctgttcaggt ctaccaccct ggggatcagt
     3061 atttgtttcc gactgaatgc cagcaattgc tgagtgagcg atgcggcgcg gttagccgcc
     3121 ttaagaatcg cctcgatcat tggacgcagc cgttcgtcgc ttccgagttg ctccttcaac
     3181 aaatcaccat aaccggtgat caccattagc atgttgttga agtcatgggc gacgccaccc
     3241 gaaagtcgcc caacggcttc catcttctgt gcttggacaa attgttcttc cagttggtga
     3301 cgctccgtga cgtcccgaat gaaaccgaga caccgaacgg gctgcccggt atcgtcgaat
     3361 tctgctttgc cggtgacttc aatccaacga actttaccgt ctggccgaat gatacggtgg
     3421 tcgagctgaa ttgactttcc aacgagcagg agaaccatgt gttcttcgac tagcgaccgg
     3481 tcttccgggt gggtgaatcg aatcgcgtct tcaaagctag gcggcggggc gttggcggga
     3541 accccgaaga tgtcacgtac ttcgggtgcc cagattagcg tgcgagttga gaattgcaat
     3601 tcccatatac ccatggaggc cacccgcaca gccagctgca gc
//
LOCUS       DASRKK010000553         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_144932, whole genome shotgun sequence.
ACCESSION   DASRKK010000553 DASRKK010000000
VERSION     DASRKK010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144932"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFC15_12795"
     CDS             complement(<1..278)
                     /locus_tag="VFC15_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZM11078.1"
                     /translation="MAALGETESILGKDLALRAGIPANYLSKVLWVLGNAEIIDATRG
                     TGGGYRLRRKAAEIRLSEVVELFDRQKPERRCLLGGSRECSDEDPCGA"
     gene            492..1103
                     /locus_tag="VFC15_12800"
     CDS             492..1103
                     /locus_tag="VFC15_12800"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZM11079.1"
                     /translation="MKQLDIETFHDFSGDEISRRILMEDPVMRVVLVSMRAGQKLPEH
                     AANGLVTVYAVSGHVLFFEGDESCDMVPGKLIGLAPGRPHRLDAMQDSRLLVTMIKPS
                     DAAAWTALAPQGRTIDLRQTPRERRHSTIFYAFDRLAVGESFLLVNDHDPKPLHAQME
                     QLRPGELSWEYEVQGPHEFRIRISRVAISATPQPETVLAGSSH"
     gene            1120..1467
                     /locus_tag="VFC15_12805"
     CDS             1120..1467
                     /locus_tag="VFC15_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11080.1"
                     /translation="MKLRLQGNSLRLRLTRSEVVRLDGHGTVEEAASFGSGGRLTYRI
                     QSRTGTEPLHADFSGGAITVLVPTETVRVWASGDEVGLYAQDGALRIAIEKDFRCLTR
                     AGEEPDAYPHPAE"
     gene            1488..>2039
                     /locus_tag="VFC15_12810"
     CDS             1488..>2039
                     /locus_tag="VFC15_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZM11081.1"
                     /translation="MPITIGAKRESDFTDPIGMLGDCHRRIERFLNVQVTLATREKGG
                     PLTEEHRAALETSLRYFREAAPKHTADEEESLFPRLRRIDPAKTQAILARVDSLEQDH
                     ECAGKAHDEVDRLGQRWLTSGRLSPEDASRLSTVLDQLAKLYRNHIAIEDTEVFPFAA
                     SILAASDREAVGVEMAARRGAGAA"
BASE COUNT          420 a          617 c          628 g          374 t
ORIGIN      
        1 tgggcgccgc aagggtcctc atccgaacac tccctcgagc cacccaggag acagcggcgt
       61 tccggcttct ggcgatcgaa cagctccacc acttcactca aacggatttc cgccgccttg
      121 cgccgtaaac ggtacccgcc accggtccct ctcgtggcat cgatgatctc tgcattgcca
      181 agcacccaca gcaccttcga gaggtagttc gcggggattc ccgcacggag cgccaagtcc
      241 ttgcccagta tcgactccgt ttcgccaagt gccgccattt cagttaacgc tcgcagtgca
      301 tgttcggccg tcgtggatag catagaagag ttgcttatga agaagtatac aactcgggtt
      361 gtggatgtca agccaaaaca attggcggag caaagcggag cctcagccgc agtcaccgag
      421 cgcagccgtt attttccaag ggcgtgactt aagtcacagg cacggctggg gggctgcgcc
      481 attctggagg tatgaagcaa ctggatattg agaccttcca cgatttcagc ggcgatgaga
      541 tctcccggcg gatactgatg gaggatcccg taatgcgcgt ggtgctggtg tccatgcgcg
      601 ccggccagaa actccccgaa cacgcggcga atggcttggt gacggtctac gcagtgagtg
      661 ggcacgtcct tttctttgag ggtgacgagt cctgcgacat ggttccggga aagttgatcg
      721 ggctggcgcc gggccggccg caccgtctgg acgcgatgca ggattcacgc ctgctggtga
      781 ccatgatcaa accgagcgac gccgctgcct ggaccgcgct ggccccgcaa gggcggacca
      841 tcgatctgcg gcagacgccg cgcgagcgca ggcacagcac gatcttttat gctttcgatc
      901 gcctggctgt tggcgagtcg ttcttgctcg tcaacgatca cgacccgaag cccctgcatg
      961 cccagatgga acaactacgc cccggcgaac tctcatggga atatgaggtg cagggtccgc
     1021 acgagtttcg gattaggatt tcccgggttg cgatctcggc aacgcctcaa ccggaaaccg
     1081 tgctcgccgg tagtagtcat tgattgcggg ctcactgaga tgaaactgag actacagggc
     1141 aactcgttgc gccttcgcct tacccgatcc gaagtagtgc gactggacgg ccacggtacg
     1201 gtggaggaag ccgcctcgtt cgggtccggc ggcaggctca cttacaggat tcagagccgc
     1261 accggcaccg agccactcca tgccgacttc agtggcgggg caattaccgt acttgtacca
     1321 accgagacag tgcgggtttg ggcgtccggc gacgaagttg gactgtatgc gcaagacgga
     1381 gccctgagaa ttgcgataga gaaggacttt cgctgcttga cgcgcgccgg ggaggagccc
     1441 gatgcctatc ctcatccggc cgaatgattt tggaagggag gcgagcaatg ccaataacga
     1501 tcggcgccaa acgggagagc gattttacag atccgatcgg gatgctgggg gactgccacc
     1561 gtcgaatcga acggtttctg aatgtgcagg tgaccctggc cacccgggag aagggcggcc
     1621 ctctcaccga agagcacagg gcagctttgg agaccagcct gcgctacttc cgcgaagccg
     1681 ccccaaagca caccgcggat gaggaagaga gcctgttccc gcgcctccgc cgaatcgacc
     1741 ctgccaaaac gcaagccata ctggcgcgag tcgattccct ggagcaggat cacgaatgtg
     1801 ccgggaaggc ccacgacgag gtggaccgat tgggacaacg ttggctcacc agtggccggc
     1861 tttcacccga agacgcatcc cggctctcca ccgtgctcga ccagttggca aagctgtatc
     1921 gcaatcacat cgccatcgaa gacacagagg tcttcccctt cgccgcgagc attctcgctg
     1981 catccgaccg tgaggccgtg ggcgtcgaaa tggcggccag acgcggtgcg ggagcggcc
//
LOCUS       DASRKK010000554         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1025647, whole genome shotgun sequence.
ACCESSION   DASRKK010000554 DASRKK010000000
VERSION     DASRKK010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025647"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VFC15_12815"
     CDS             complement(<1..1709)
                     /locus_tag="VFC15_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZM11082.1"
                     /translation="MKIRCICVALLLLCLGSLPSTAQSSAQDKEIQLPSSKLLLLPAP
                     GGPQRTNSFPTAVALSPDGKYLAILNNGYGTAESKFQQSIALLDLASNQLRDFPDPRL
                     ALHAKQSYFLGLAWSSDGASLYASMASLTDPEGKNPGDTGNGVAVYRLQIGALLADRF
                     LKLPRAPLGRGKRFTYNPKSVAPGNANPYPAGLAVVKRKGGDALLIAENLADDAVLLD
                     AHSGKVLQRFDLGRGKYVPAAFPYGVVVSADGSRGWCSLWNASQVAELDLRSGKVVRQ
                     IALLPPQRDIDASSHPTALLLSPDQRHLYVTLSNRDSVGVVSTADGHVERYLSTSLPG
                     QTYGGSYPDALAQSADGSKLYVANASADAIAVFDMEKQAARPKASSLGGAATDANLKD
                     GSANGQSAEYFIPTEWYPTAVAVHGGELLIASGKGEGTGPNAGWQDAPDRPGKRRHPY
                     IATMIRGSIARVSLAEAEINRDKLTQAVVRGNQMEGRTGEISFQRGENPIHHVIYVIK
                     ENRTYDQLFGDIAEGNGDPSLVMYGEDITPNQHKLARQFGILDNFYDSGEVSGNGHVW
                     STAA"
     gene            complement(1736..2386)
                     /gene="udk"
                     /locus_tag="VFC15_12820"
     CDS             complement(1736..2386)
                     /gene="udk"
                     /locus_tag="VFC15_12820"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HZM11083.1"
                     /translation="MPAEIYLIGIAGPSGAGKTYLATHLAAALHASVLALDRYYRDLS
                     HLPLEERARMNFDAPEVLDHELIVDQVAGLRNNETVRLPVYDFATHTRTRRTEVLRPA
                     DVVILEGLFTLHWPRLRELLGTKVFVDMNDEVCLARRQERDVRERGRTPESVVQQYAA
                     TVAPMAYRYVRPTIVHADVVVSGNEPIGDGVSRVLAHYRRQVANGKAHEPAGQAQR"
BASE COUNT          510 a          757 c          752 g          494 t
ORIGIN      
        1 gccgcagtgg accacacgtg cccattgccg gagacctcgc cgctatcgta gaagttgtcg
       61 agaattccaa actgccgagc cagcttgtgc tggttcggag tgatgtcttc cccgtacatg
      121 accagcgagg gatcgccgtt cccctccgcg atgtcgccaa agagctgatc gtaggtgcga
      181 ttttccttga tcacgtaaat cacgtgatga attggatttt cgccgcgctg aaagctaatc
      241 tcgccggtgc ggccttccat ctggttgccg cgaaccactg cctgggtgag tttgtcgcgg
      301 ttaatttccg cctcagccaa cgatacccgc gcaatcgagc ccctaatcat ggtcgcgatg
      361 tagggatgcc ggcgttttcc cggacggtcg ggggcgtctt gccaaccggc gttggggccg
      421 gttccctccc ctttgccact tgcaatcagc agctcgccac cgtggaccgc cacagccgtc
      481 ggataccact ccgtgggaat gaaatactca gcgctctgtc cattggcaga gccgtctttt
      541 aggttcgcgt ccgtcgcggc gccgccaagc gagctggctt ttggccgggc ggcttgcttt
      601 tccatatcga atactgcgat ggcgtcggca gatgcattgg cgacgtataa cttgcttccg
      661 tcagctgact gcgcgagcgc gtcgggatag ctgccgccat aggtctgtcc gggcaagctg
      721 gtgctgagat aacgctcaac gtggccgtct gcggttgaca cgactccaac cgaatcgcgg
      781 ttggagagcg tcacgtagag atggcgctgg tccggactca acaggagcgc tgtcggatgc
      841 gacgaggcat cgatatcgcg ctgcggaggc agcaacgcga tctgccgtac aactttgcca
      901 gagcgcagat ccagttctgc gacttgcgat gcgttccata gcgaacacca gccccggctg
      961 ccatcggcgc tcaccacaac gccgtacgga aaggcggcag gtacgtactt gccacggccc
     1021 agatcgaagc gttgcagcac cttgccgcta tgcgcatcca gcaacaccgc atcgtctgca
     1081 aggttctccg cgatcaacag cgcatcgccg cccttgcgct tcaccacagc cagtccggcg
     1141 ggatacgggt tagcattacc gggcgcaaca ctcttgggat tgtaagtaaa tcgtttgccg
     1201 cggccgagcg gcgcccgagg cagtttcaga aagcggtccg ccagcagcgc gccgatctgc
     1261 aagcggtaca cggcgactcc gttccctgta tcgccgggat tcttgccttc ggggtcggtc
     1321 agcgatgcca tcgaggcgta aagactggcg ccatcgctgc tccaagccag accaagaaaa
     1381 tacgattgct tggcatgaag cgcgagacgc gggtcgggaa agtcccgcag ttgattgctc
     1441 gccaagtcca gcaaggcaat cgactgttga aacttggact cggccgtccc atatccatta
     1501 ttcaatatgg cgaggtactt cccgtccgga ctgagtgcca ccgccgtggg aaagctattg
     1561 gtgcgctgcg gccctcccgg cgcaggcaac agcagcaact tgctagaggg cagttgtatt
     1621 tccttatcct gagcagatga ttgagcagta gagggcaggc tccccaggca cagcaacaaa
     1681 agagcgacgc agatgcagcg gatcttcatt cgttcctcgt cattgcaggc atggcttatc
     1741 gctgcgcttg accagccggc tcgtgagcct tgccgttggc aacctgccgc cggtagtgcg
     1801 ccagaacgcg ggatacgcca tcgccaatgg gctcattccc ggagaccacc acatcggcat
     1861 gaacgatggt gggacgaaca tagcgatagg ccatgggagc gacagtggcg gcatactgct
     1921 gcacgacgga ttctggagtg cggccccgct cgcgtacgtc gcgctcctgg cgacgcgcca
     1981 ggcagacttc gtcattcatg tccacgaaaa ccttggtgcc cagcaactcg cgcagacggg
     2041 gccaatgcaa agtaaatagg ccctcgagga taacgacgtc cgcgggacgc agcacctccg
     2101 tcctccgggt ccgcgtgtga gtagcgaaat cgtacacggg caagcgcaca gtttcgttgt
     2161 tgcgcaagcc agcaacttgg tccacgatga gttcgtgatc gagaacttcg ggcgcgtcga
     2221 aattcatccg cgctcgttcc tccaagggca gatgcgagag gtcccgatag tagcggtcaa
     2281 gcgccagcac gctggcatgc agcgcagcgg caagatgcgt ggccaggtac gtttttcccg
     2341 cgcccgaagg accggcaatg ccaatgagat atatttccgc gggcatgtgc aatgtagtgt
     2401 agcgcagagg cccccaagcc ttgtgacgat aacgttaatc ggaattggaa aggtcattca
     2461 gataaccgtc gatcctgggc aacaccgcgc gcaatagcgc aaggagcttg ccg
//
LOCUS       DASRKK010000555         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_732885, whole genome shotgun sequence.
ACCESSION   DASRKK010000555 DASRKK010000000
VERSION     DASRKK010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732885"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFC15_12825"
     CDS             complement(<1..687)
                     /locus_tag="VFC15_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM11084.1"
                     /translation="MVVASKPDLHAELQEQLRFERLIADLSARFVNVPADRVDREIED
                     AQRAICECLRLEHSSLWQASIQNPGELVMTHIYRDPELPPTPERMSATEFFPWAQAKL
                     FAGEIIAVPNTAEVPPEASTDREAWHRFGVKSTLGFPLSVGGAPVFGVISFDATREVR
                     ACPEPLLQRLQLLAQVFANALERKRADQKLRDSEARLSLAATSANAGLWTLEPETGHI
                     WATEKTYELLG"
     gene            complement(950..1210)
                     /locus_tag="VFC15_12830"
     CDS             complement(950..1210)
                     /locus_tag="VFC15_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11085.1"
                     /translation="MQEYTNSNAGELATLAQEARAHFEQRVSTRETGDSYVKVTVFLA
                     TVLLLTALSQRVEVVGPRVAVIAVAFLLLIISTYWIVILPRA"
     gene            complement(1341..2651)
                     /locus_tag="VFC15_12835"
     CDS             complement(1341..2651)
                     /locus_tag="VFC15_12835"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZM11086.1"
                     /translation="MVVFDADPAYAGVSRQYLARAALQRIQEAVIDYRHDREPRLLVR
                     HGQLAAGATLALIVGLWIARVVVRKSRSALERRYKEKVHGIQIQSFRLIEAERLWRVM
                     ASAHGLVSAVIALAMAYLYAHYVLALFPWTRGLANSLSDFLVNPLIAIGTAFLNAVPN
                     LIFLAILILVTWYVLKLIRVFFLGIDTGAITLSGFEAAWAKPTYRLVRFAVIAFALVA
                     AYPYIPGSDSQAFKGVSLLVGVVFSLGSTSLIGNMISGYSMAYRRLFKNGDRVKIGNH
                     IGDVEETRLMVTYLRTIKNELVAVPNSSIINEEVVNYSALAHKEGLILHTTVGIGYQT
                     SWRQVEGMLIHAAERTPGLLREPKPFVMEKELGEFAITYEINAYCDNPQAMERLYAAL
                     YASILDVFNEYGVQIMTPAYIADPPQAKIVPRDQWYAAPAQPPD"
     gene            complement(2694..3518)
                     /locus_tag="VFC15_12840"
     CDS             complement(2694..3518)
                     /locus_tag="VFC15_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11087.1"
                     /translation="MKLTKDINPKLPWFVILLATVMLTAAVEPSFAQATSSAMPKVFS
                     DGADAKSKRIDNLNQVRYIEIYLAAPDAKTGKLVAACYNSSVLPSEIPASKDTSPQPL
                     VEGLDLAKFKKEYGVLDASLNGPKLWMLDWIEIDMGVERDFNGIKASWCAQLNMDKNA
                     NVNKTPAYNPVTIARKISVGWNKGTTALLLDDAQGNTWIMKGFQQGLKPQHTYQQFVA
                     AGASNFKKLPPGWKYRVKTLDKDYIETPENGIATIMADEFFNIYDKTGPGMGNYKP"
     gene            complement(3557..>4783)
                     /locus_tag="VFC15_12845"
     CDS             complement(3557..>4783)
                     /locus_tag="VFC15_12845"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM11088.1"
                     /translation="YKGPFNQVLNTAQVFTPKDTAVTTPNSDTPYSFLQADLRAEPIV
                     FCVPKIEKNRYYSVQLTDMYSFNYGYVGSRATGNDAGCYMVAGPGWSGESPKGIKKVF
                     RSETQFSMIIFRTQLFSPADIVNVKKIQAGYTAQPLSAYLKQPAPLAAPAIDFPKFTG
                     DAFKLDFPKYLNFLLQFCPEVPEEKALRAKFAEIGIAPGKPFESDKISDVQKGELGLG
                     VKEGYDAIEKQGADIGKDVNGWRIGAPFGDRAFFHGDYLLRASAARLGLYGCDPAEAM
                     YPLTRNEGTGAALDGSKHSYTLTFAAGQLPPVNSFWSVTMYDGKTQLLVENALNRYLI
                     NSPMLSTLKKNKDGSLTLYIQKDAPSADSKTNWLPAPDGPIYMVMRLYWPKTTPPSIL
                     PPGDGTWKPPAVIVVQ"
BASE COUNT         1007 a         1333 c         1363 g         1080 t
ORIGIN      
        1 cccgaggagt tcgtacgtct tctcagttgc ccatatgtga cctgtttctg gttccagggt
       61 ccacaagccg gcgttggcag atgtggcggc cagactcagc cgtgcttcac tgtcgcgcag
      121 cttttggtcg gcgcgctttc gctcgagtgc gtttgcaaat acctgcgcca gcaattgaag
      181 ccgttgcagg agcggttcgg gacaggctcg tacctctcgg gttgcatcga aactgatgac
      241 accgaatacc ggagcgcccc caaccgaaag cggaaatccc agagttgatt tcacgccaaa
      301 gcggtgccat gcttcacggt cagtgcttgc ctccggcggg acttcggcgg tgttggggac
      361 tgcaatgatc tcaccagcga agagcttcgc ctgagcccag gggaagaatt ccgtggcact
      421 catacgctca ggcgtcggcg gtaactccgg atctcggtaa atatgggtca tgaccaattc
      481 gccgggattc tggatcgagg cctgccagag cgaggagtgt tccaatcgca aacactcgca
      541 aatcgcgcgc tgggcatctt cgatttcccg atctacacga tcagcgggaa cgttcacaaa
      601 acgcgccgag agatccgcaa taagtcgctc aaaccgcagt tgctcttgca gttcggcgtg
      661 gagatccggt ttggatgcta cgaccatatt caagacctcg gatcgggcaa atcccaccaa
      721 ccctgagccg attggaagac cgcacgtctt gaggatgacc tgggctgaac tgctcagttt
      781 atcccatggg ttgacgaaag tgacgtgggc gcactgatta ctcaataccg ggaatcgacc
      841 ggcttacacc cccggccgat ttccgcaatc tgagccagca gtcgatttga acgtcgcgga
      901 gagggtaact cgcgcttggc agaccagtgt ttatgatcct ttccgcgggt catgcgcgag
      961 ggagaatcac gatccaatac gtgctgatga tcagcagcaa gaacgcaacc gctattaccg
     1021 cgactcgcgg ccctacgact tcgacccgct ggctgagcgc agtcagaagt aacaccgtcg
     1081 ccagaaagac cgttactttt acgtagctgt ccccggtttc ccgcgtgctg accctctgct
     1141 cgaagtgagc cctggcctcc tgagccagcg ttgccaactc tcctgcgttt gagttggtgt
     1201 actcctgcat gaagatcgcg acgggcggcg cagacggatc gttaaacggg tcgagtttct
     1261 gccaggctgc aaacgcggca gcgtattccg gatggaatcg gcgctggtag attgcggtga
     1321 gtttatcgtt gcctgccgcc ctaatccggc ggctgtgcgg gtgcagcgta ccattggtct
     1381 ctcggaacga tctttgcctg cggcggatca gcgatgtagg ccggcgtcat aatctgcacc
     1441 ccgtattcat tgaagacgtc gaggatactg gcgtacaacg cggcatatag ccgctccatg
     1501 gcttgggggt tgtcgcagta agcattgatc tcgtacgtga tcgcaaactc cccaagttct
     1561 ttttccataa caaaaggctt cggttcgcgc aggagtcccg gcgtgcgttc tgctgcgtgg
     1621 atcagcatgc cctccacctg gcgccaggaa gtctgatacc cgattccgac cgtggtatga
     1681 agaatcaatc cctctttgtg ggctagcgcg ctgtagttca ctacttcctc gttgatgatg
     1741 gatgagttcg gaaccgcgac caattcattc ttgatggtgc gcaggtacgt caccatcagc
     1801 cttgtctctt ccacatctcc gatgtgattg ccaatcttga cacggtcgcc attcttgaac
     1861 agacggcgat acgccatgct gtatccggag atcatgtttc caatcaagga ggtcgatccc
     1921 agggagaaaa ccacaccgac cagtagggag acgcccttga aagcctgcga atccgaaccg
     1981 ggtatgtagg ggtaagcggc caccagcgcg aaggcgatca cggcaaaccg caccaggcga
     2041 taggttggct tcgcccacgc ggcctcgaac cctgacaagg tgattgctcc ggtgtcaatg
     2101 ccaagaaaaa aaacgcgaat cagcttcaac acataccagg tcaccaggat cagaatcgcc
     2161 aggaagatca ggttgggaac cgcgttcagg aacgcagtgc caatggcgat caagggattc
     2221 accaggaagt cagacaagct gttggctagt cctcgcgtcc aggggaacag tgccagaacg
     2281 taatgcgcat aaagataggc catcgccagg gcgatgactg ccgagaccag cccatgcgcg
     2341 ctagccatca cccgccataa acgttcggcc tcaatcaggc ggaacgattg aatctggatt
     2401 ccatggacct tctccttgta acgcctctcg agcgcagagc gggattttct gaccactaca
     2461 cgcgcgatcc atagaccaac gatgagtgcc aacgtcgcac cagcggccaa ttggccatgt
     2521 cggaccagga gcctcggctc gcggtcgtgc cggtagtcga tgaccgcttc ttggattcgc
     2581 tgcaaggcag ctcgggccag gtactgccgg ctcacacccg cataggcagg gtcggcatca
     2641 aaaacgacca tgatggtttg gtcgccagct agtatccgtg ttccaaacga taattatggt
     2701 ttgtagttgc ccattcccgg cccggtcttg tcataaatgt tgaaaaactc gtccgccatg
     2761 atcgtggcaa tgccgttttc cggcgtctcg atgtaatcct tgtccagcgt cttgacccgg
     2821 tacttccagc ccggcgggag cttcttgaag ttgcttgcgc ctgcggcaac aaattgctgg
     2881 taggtatgct gcggtttgag gccctgctgg aaccccttca tgatccaggt gttgccttga
     2941 gcatcatcaa gtagcaacgc cgtggtgccc ttattccagc ccactgagat tttccgtgca
     3001 atggtcacgg gattgtaggc cggcgttttg ttcacgttgg cgttcttgtc catgttcagt
     3061 tgagcgcacc acgaggcctt gatgccgttg aagtccctct ccactcccat atcaatctca
     3121 atccaatcga gcatccagag tttcgggcca ttcaacgacg cgtcgaggac accatattct
     3181 ttcttgaact tggctaaatc gaggccctcg accaacggct gcggcgaggt gtccttcgaa
     3241 gcggggatct ccgagggaag gacggaggag ttgtagcagg cggcgaccag cttcccggtt
     3301 ttcgcgtcgg gtgccgcgag gtagatctcg atgtagcgca cttggttcag gttgtcgata
     3361 cgttttgatt ttgcgtcggc gccgtcggaa aacactttgg gcatcgccga ggacgttgct
     3421 tgtgcaaaag acggttccac ggccgccgtc aacatgacgg tagccagcaa aataacaaac
     3481 cacggcaatt tcgggttgat atccttggtc agtttcatat gctccttttc gcatttaact
     3541 tagaatcgaa tcctcattac tgcactacga ttaccgccgg cggcttccag gtgccatcac
     3601 ccggaggcaa gatagaaggt ggcgtcgtct tgggccaata gaggcgcatc accatgtaaa
     3661 tcgggccgtc gggtgcgggt agccagttgg tcttactatc agctgatggg gcgtccttct
     3721 ggatatacag agtcagcgag ccgtctttgt ttttcttcag ggtcgagagc atcggcgaat
     3781 tgatgaggta acggttgaga gcgttctcga cgagcaattg cgtcttgccg tcgtacatgg
     3841 tgacggacca gaacgaattc actggcggga gctgaccggc ggcgaaggtc agcgtgtaac
     3901 tgtgcttgct gccgtcgagc gccgctcccg tgccttcgtt tctggtcaag ggatacatgg
     3961 cttcagccgg atcgcagcca tagagtccca agcgcgctgc tgaggcacgc agcagatagt
     4021 cgccgtggaa gaaagcgcgg tcgccaaagg gcgcgcctat tcgccagccg ttgacgtcct
     4081 tgccgatgtc cgctccctgc ttctcaatgg cgtcgtaacc ttccttcaca cccaggccca
     4141 gttcgccctt ctgcacgtct gagatcttgt ctgactcaaa cggcttgccc ggggcgatgc
     4201 cgatctcggc gaacttcgcc cggagtgctt tttcctcggg gacttcgggg cagaactgga
     4261 gcaggaaatt cagatacttg ggaaaatcca acttgaaggc gtctccggtg aacttgggaa
     4321 agtcaatcgc cggagccgca agaggagctg gttgcttcag atatgctgaa agcggctgtg
     4381 ctgtgtaacc tgcctggatc ttctttacat tgacgatatc cgccggactg aacagttgcg
     4441 tgcggaaaat gatcatgctg aactgcgtct cggaacgaaa gaccttcttg attcccttcg
     4501 gggattcgcc gctccagcca ggtcccgcga ccatgtagca gccggcatca ttacccgttg
     4561 ctcgcgagcc aacatagccg tagttgaacg agtacatgtc ggtcaattgc acggagtagt
     4621 aacggttctt ctcgattttg ggcacacaga aaacgatcgg ctcagcgcgg aggtcggcct
     4681 gcaaaaacga atatggcgtg tcgctgttag gtgtggttac agctgtgtcc ttgggtgtga
     4741 agacctgcgc cgtgttcagg acttgattga atgggccttt ata
//
LOCUS       DASRKK010000556         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_48471, whole genome shotgun sequence.
ACCESSION   DASRKK010000556 DASRKK010000000
VERSION     DASRKK010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48471"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..2165
                     /locus_tag="VFC15_12850"
     CDS             393..2165
                     /locus_tag="VFC15_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM11089.1"
                     /translation="MFLRSTNRKKDGKDHRYFSVVENQRVSSHKTVQRTVLYLGEIND
                     QQQASWRKTISVFDEDQQQFTNLSLFPEDSPLPNDAVDSLQVRLSGLELRRPRPFGNC
                     WLANDLWQQLGLTEFWNQHLPEGREAVSWEKVLRLLVGNRLLEPGSEFRVHRHWFVES
                     AMDALLETDFVVADKDRLYRCLDRILEHKQDLFIWLKRKWAELFHADFEVLLYDLTST
                     YFEGEMEENPKAKRGYSRDSRPDCVQVVIALIVTPDGFPLAYEVMDGNTSEHKTLSPF
                     LRHIEDTYGRARRVWVMDRGIPTEATLKEMREPGRDMFYLVGTPKGRINKHEKKWLDL
                     PWQQVRDSVQVKLYQHEGELYVLARSDGRQAKEIAIRRKRLARLLRKLRAMRRSLPSR
                     DQLLLRIGAARKEAGRAFGFVKIHLPLAEETVSRKSFHFAVDKTKLRAAQQRDGHYLL
                     RSNLTAEDPAVLWTRYVQLTQIEAVFRSLKSDLGLRPIYHRLEHRVDAHIMVAFLAYC
                     LQITLKNRLQIHAPGLSPTAVLEKLATIQMIDVWIPTRDGRWLILPRYTQPAKDLQVL
                     MQQIHLTLPSQPPPRITALPPAGR"
BASE COUNT          536 a          724 c          704 g          499 t
ORIGIN      
        1 cccaggcgtc cttgcggagt gagtgacgcc aggggtacaa cgctcggctg gtgcttcggc
       61 gcccgtaaca gccctttcca caaaggatct ccttctgtcc cgactacgcc gtcgataacc
      121 aacccacttg gctggtttgt cgttcgactc gccgaagtgc tcgccgaacc ccgcgcctac
      181 tctttcgcgt atggcagcac cgaggctgtc gcccaggatg ccggctgtga actaccgatg
      241 ttgtccgtta acctgacttt cgcactccag aggcattttg cccatcctgt gccagctaac
      301 ccaagcgcct gcaacgcgga atttcggaaa acctcagagt gaagacctac cctcttccat
      361 aacgcgaaaa actctggcat tctgctgttg ccttgttcct acgcagcaca aaccggaaga
      421 aagacggcaa agaccatcgc tacttcagcg tcgtcgaaaa ccagcgcgtc tcctcccaca
      481 aaaccgtgca gcgcactgtc ctgtatctcg gcgagatcaa cgatcaacag caagcatcgt
      541 ggcgcaagac gatcagcgtc ttcgacgaag accaacagca gttcaccaac ctgagtctgt
      601 tcccagaaga cagcccgctg cccaacgacg cagtagatag cctgcaagtg agattgagcg
      661 gcctggaact gcggcgcccg cgacctttcg gcaactgttg gttggccaat gatctgtggc
      721 aacagttggg cctcaccgaa ttctggaacc agcatttacc ggaaggccgc gaagcagtga
      781 gttgggaaaa ggttctgcgc ctgctggtag ggaaccgatt gctggagccg gggagcgagt
      841 ttcgtgtgca ccggcactgg ttcgtagaga gcgcgatgga tgcgctgctg gaaaccgatt
      901 tcgtagtggc ggacaaagac cgcctctatc gatgcctcga ccgcatcctg gaacataaac
      961 aggacctgtt tatctggtta aagcggaagt gggccgaact gttccacgcg gacttcgaag
     1021 tattgctgta cgacctgacc agcacctact tcgaaggcga gatggaagag aatcccaaag
     1081 ctaagcgggg ctatagtcgc gatagccgtc cggattgcgt gcaagtggtg atcgcgctga
     1141 tcgtcacgcc cgacggcttc cctctggcct acgaagtaat ggacggcaac acgtcagaac
     1201 acaaaacgct gagcccgttt ttgcgccaca tcgaagacac ctacggcaga gcgcggcggg
     1261 tatgggtgat ggatcgcggc attccgacgg aagctacgtt gaaagagatg cgcgagccgg
     1321 gacgcgacat gttctacctg gtgggtacac ccaagggccg gatcaacaag cacgagaaga
     1381 agtggctgga cctaccgtgg caacaagtgc gcgacagcgt gcaggtcaag ctctaccagc
     1441 atgagggaga gctctatgtg ttggcgcgga gtgacgggcg ccaggcgaaa gaaattgcga
     1501 tccgccgcaa acggctggct cgcctgctgc gcaagctacg cgcgatgcgg cgcagtttgc
     1561 cgtcacgcga tcaactgctc ttacgcatcg gagcggccag gaaggaagcg gggcgggcat
     1621 tcggtttcgt gaagattcac ttgccgctgg cggaggagac agtttcgcga aagagctttc
     1681 acttcgcggt cgataagacc aaactgcgcg ccgcacaaca acgcgatgga cactatcttc
     1741 tgcgctcgaa tctcacggca gaagatcccg cggtgttgtg gactcgctat gtgcagctga
     1801 cacagatcga ggcggtcttt cgctcactga aaagcgatct cggcctgcgc cctatttacc
     1861 atcgattgga gcatcgcgtg gatgctcaca tcatggttgc cttcctggcc tattgcctac
     1921 aaatcacgct gaagaatcgt ttgcagattc acgcgccagg gttgtcgccc acggcagttt
     1981 tggaaaagct cgccacgatt cagatgattg acgtctggat tccgactcgc gatggccgct
     2041 ggttgattct cccgcgctac actcaaccgg ccaaggatct acaagtactg atgcagcaga
     2101 ttcacctcac gctgccctcc cagccgcctc cacgaatcac agctctccct cctgcgggcc
     2161 gctgaagggc tcgttgatca accccattct gtggtgaaga cctttgggtc gcctcgattg
     2221 attccgtgcc acttaacccc tccgttcggc tccaagtgcg aaagtcaggt taacgccgct
     2281 ttcgcacggt ttggtgatgc ctgcctgggg gattgctggt ggcgagaatt tcgcgtcact
     2341 agcgggccaa cgtcgcgttg agccgcgcag cgccagtcgc cgtgatcggg acttaaccgg
     2401 gagtagtctc gtagcgccgt ttggggccac ttatccgaca agccaccata cgtcttaggt
     2461 tag
//
LOCUS       DASRKK010000557         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_830072, whole genome shotgun sequence.
ACCESSION   DASRKK010000557 DASRKK010000000
VERSION     DASRKK010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830072"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..348)
                     /gene="trxA"
                     /locus_tag="VFC15_12855"
     CDS             complement(19..348)
                     /gene="trxA"
                     /locus_tag="VFC15_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZM11090.1"
                     /translation="MASEAVLEVTDANFDQAVLKSDQPVLIDFWATWCGPCRALAPIV
                     DEVAQTYSGRVKIGKMDVDKNVATPQRYGVRGIPTLLIFKGGQVKEQIVGYVSRDVIE
                     KALDKNL"
     gene            complement(809..>2822)
                     /locus_tag="VFC15_12860"
     CDS             complement(809..>2822)
                     /locus_tag="VFC15_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZM11091.1"
                     /translation="SVVANGGHFFLAKTAADANRYILDVLQRHQVKLVVKGKSITSEE
                     IRLNPTLEAAGITVAESDLAEFILQVADEQPSHNVAPALHYSRERITALFKRKFDTDL
                     PLDTGEELTRFAREILRQKFIAADAGITGANLIAADSGSLMLVESEGNIRMTTTLPPV
                     HIAIAGIEKVVPSRSDFQSFIELLPASATGQQLTSYVSILRPPLPALPFVAPGKTAQP
                     REFHLVLIDNGRSAMREDPVLYEALYCIRCSACLNVCANFETVGGHAFGGETYSGGIG
                     GSWEAGTGKLMNARFSELCTGCSRCVPQCPVRIDIPWLNQNLRQRMNQTQENSAVKSL
                     FGSLTGSAPQDKAVSLQKQFFGNYHTVGKWGTQFASLANGSTRLPMARDVMENVFGVD
                     HRRELPAFPAKTWEQLFDEEQRRGVKAGTIEPGVVMLADTFTNYGSPERGMAAVRVLR
                     AIGVDVVLSPSIPDGRAAMSQGMIETARMQARAMSEMLRPFLDSGRKIVIVEPSVLAM
                     LRFDFKHLLDDESAVVALAANSFEPLQAVWDIATETGLDLAKLFPAAKSRFGTRLFYH
                     AHCQQKTCNAASQTIELLRAAGFDVVTSAVECCGMAGSFGYKHDYYELSMAVGEDLFA
                     QVRQADLEGGPRVLVASGVSCHEQLTAGTGRAILHPAELLQSTLEG"
BASE COUNT          585 a          852 c          801 g          584 t
ORIGIN      
        1 agaaactttc agcccgtttt acaggttctt atcgagcgcc ttctcgatga cgtcgcgcga
       61 cacgtagccg acgatctgct ccttcacctg tccgcctttg aagatcagca acgtggggat
      121 gccgcgcacg ccatagcgct gcggggtggc aacgttcttg tccacgtcca ttttgccgat
      181 cttgacccgg ccactgtagg tttgagccac ttcatcaacg atgggcgcca gggcccggca
      241 ggggccgcac caggttgccc agaagtcgat cagcacaggc tggtccgact tcaatactgc
      301 ctggtcaaaa ttcgcgtccg tcacttccaa aactgcttca cttgccatgt atcctcctga
      361 gggtcgccgc tggtccatgc gacaaaaagc cgagccacgg ttctcatcgt accattcgat
      421 tgcttcgtga ctggaacagg tagttagatg cggcggcgag tagacggttg catgtcggcg
      481 ctaatgaggt cccacttgca catgaagttc accaagagtc gcagcgaaag cgaactcagt
      541 cttgcgcaat atgacctgaa atcaaaagcg ctccgcgtcg attcgcggag cgcctagaag
      601 gcagccctcc cccagaactg cctaactacg cctcagatat tgcgccgaac ccgccggcat
      661 gtaaatacat cttccgtaac ttaaaggtaa tctgcagtaa tttgacagct gtgacgtatc
      721 taatacaaaa tattgaattt ttggtcgtcg ggctctccaa ctctcgcgga tatggcctat
      781 tctggccaat tactacgcag caccgtagtc acccctcaag ggtactctgc agcagttccg
      841 ccggatgcag aatggcgcga cccgttcccg ccgtaagctg ctcatgacag gagaccccgc
      901 tggccaccag cactcggggg cctccctcca ggtccgcctg gcgcacttgg gcaaacaggt
      961 cctcgccgac cgccatgctg agttcgtaat agtcgtgttt gtagccgaag cttcccgcca
     1021 taccgcagca ctcaaccgca gaagttacaa cgtcgaaacc cgccgcccgc aaaagttcga
     1081 tggtctgcga tgcggcattg caggtcttct gctggcagtg agcgtggtag aacagcctgg
     1141 ttccaaaacg cgacttggcc gctggaaaca gtttcgcaag gtcgagaccg gtttcagtgg
     1201 cgatgtccca cactgcctgc agcggctcaa agctgttcgc cgccagagct acaacggctg
     1261 attcgtcgtc cagcagatgc ttgaagtcga agcgcagcat ggccagaacg ctgggctcaa
     1321 cgataacgat ctttctgccg ctatcaagga agggccgcaa catctcggac atcgcacgag
     1381 cctgcattct cgcggtttcg atcatgcctt gcgacatggc cgcgcggccg tcggggatcg
     1441 acggactcaa aaccacgtca acgccaatcg cgcgcagcac ccggaccgcc gccattccgc
     1501 gttcaggcga accgtaattc gtgaacgtat cggccaacat cacgacgcct ggctcgatgg
     1561 ttccggcttt cactccacgc cgttgctctt catcaaacag ttgctcccag gtttttgccg
     1621 ggaacgcagg caactcgcgg cggtggtcca cgccaaaaac attttccata acgtcccgcg
     1681 ccatcggcaa gcgggtagac ccattcgcta gcgaggcgaa ctgcgtgccc catttcccga
     1741 cggtgtgata gttgccgaag aactgcttct gcagggacac ggccttatct tgcggtgcgc
     1801 tgccggtgag cgaaccgaag agcgacttca ctgcagagtt ttcctgcgtc tggttcatgc
     1861 gctggcgcag gttttgattg agccagggga tatcgatgcg caccgggcat tgcggcacgc
     1921 agcgcgagca tcctgtgcaa agttcgctga agcgcgcgtt cattagcttg ccggtgccgg
     1981 cttcccacga gccaccgatg cctccggaat aggtttcgcc tccgaaggcg tgtccgccga
     2041 ccgtctcgaa attggcgcag acatttagac aagcactgca gcggatgcag tacagagctt
     2101 cgtataacac cggatcctcg cgcatcgcgc tgcgcccatt gtcgatgagt accagatgaa
     2161 attcgcgcgg ctgcgcggtc ttccccggtg ccacgaacgg caacgccggt agcggcgggc
     2221 gaagaatgct gacatacgag gtcagttgct ggccggtcgc gctggccgga agcagctcga
     2281 tgaacgactg aaaatcagag cgtgacggca cgactttttc gatgcctgct atcgcgatgt
     2341 gcaccggcgg gagagtcgtg gtcatgcgaa tattgccttc gctttccacc agcataagcg
     2401 aaccgctgtc ggccgcaatg agattggctc ccgtgatgcc ggcatcggcg gcaatgaact
     2461 tctggcgcag aatctcgcgg gcaaagcggg taagttcctc acccgtatcg agaggcagat
     2521 cggtgtcgaa cttgcgtttg aacagcgcgg tgatgcgttc gcgcgaatag tgcaacgcgg
     2581 gcgccacatt gtgggacggc tgctcgtcgg caacctgcag gatgaattca gccagatcgc
     2641 tttccgccac cgtgatgccg gcggcctcga gcgtgggatt gagacgaatc tcctccgagg
     2701 tgatactttt ccccttcacc acgagtttga cctggtgccg ctgcagcaca tcgaggatgt
     2761 agcgattggc atcggcagcg gtcttggcga ggaaaaagtg gcctccgttg gcgacaacgc
     2821 tg
//
LOCUS       DASRKK010000558         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_634254, whole genome shotgun sequence.
ACCESSION   DASRKK010000558 DASRKK010000000
VERSION     DASRKK010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634254"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1732
                     /gene="istA"
                     /locus_tag="VFC15_12865"
     CDS             188..1732
                     /gene="istA"
                     /locus_tag="VFC15_12865"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM11092.1"
                     /translation="MDWRAKVELYEQIRLEYEFGVGTIQGVSRKLQVHRRLVREAIRS
                     AIPARRKKTERPHVKMAPAAEFIDAILETDRKAPRKQRHTAKRIWERIRQEVPGCTPA
                     ERTVRQYVAQRKEALGLIRRETFVPQSYDWGVEGQVDWYEAYADLDGERTKLQVFSMR
                     SMASGAAYHRAYLRATQQAFLEAHELAFHYFGGVFRRLRYDNLSSAVKKILRGQEREL
                     TARFIAFRSHWQYQADFCTPGEGHEKGGVEGEVGYFRRNHWVPVPVARDLAELNAKLL
                     ADCRQDESRVLSGRVEHVGMLLLAEKQHLRPLPAEDFELAEVSFPRVNQAGCAKVRTN
                     FYSVPMQAGSVVEARAYSSVVEFRQDGVRVAQHQRSYERAQQVLDLEHYLEVLERKPG
                     ALRGSKPLAQWRAQGRWPESYDQLWELMNERHGRQEGTRSMIALIRMGREFSYAKLET
                     SVAQAMQLGCTDVAAIRHLLMTDELQHATVTTVEIGALAAYERPLPTMAEYNQLLSER
                     TIEVQA"
     gene            1729..2484
                     /gene="istB"
                     /locus_tag="VFC15_12870"
     CDS             1729..2484
                     /gene="istB"
                     /locus_tag="VFC15_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM11093.1"
                     /translation="MSLEAASIAQHCQTLRLTAIGTQFLSLAEEASQHNHSHLHYLEA
                     LLQAEVEDRQRRSIELRLKDAHLPRMKTLEEFDFGQSPNIPASRIRALAEGGYLERSE
                     PVLLVGEPGTGKTHLATGLAIAACRQRRRVRFTTAAALVNQLVEAQREQSLSRMLARW
                     SRVELIVIDELGYVPLAEVAAELLFQVIAERAEKAAIIVTTNLPFSEWPQVFTNARLC
                     KAVLDRLTDQAHIIETGSESYRFRRTLRKKRKE"
BASE COUNT          575 a          796 c          874 g          528 t
ORIGIN      
        1 catgtcactc atcgcaccgg ctgtaaacgt tcgcctgaag tcggtcctgc tcgcgactga
       61 tttctcagaa gcatctgctg attttggcat tctgaagtgg tccacttcgg cggtttgttt
      121 tggcccacct cggcatagca aacttctatt ctggatttga gcgtgagctt gggtctggag
      181 gcgggagttg gactggagag ccaaggtgga gttatacgag cagattcggc tggagtatga
      241 gtttggggtg ggcacgatcc agggtgtgtc gcgaaagctg caggtgcacc ggcgattggt
      301 gcgggaggcg atccgaagcg cgataccggc gcggcggaag aagacggagc gaccccatgt
      361 gaagatggct ccggcagcgg agttcatcga cgcgatactg gaaacggatc gcaaggctcc
      421 gcgtaagcag cgccacacgg caaagcgcat ctgggaacgc attcgacaag aggtgcccgg
      481 atgcacgcca gcggagcgca cagtgcggca gtatgtggca cagcgcaaag aggcattggg
      541 tctgatccgg cgcgagacct ttgtgccgca gagctacgac tggggggttg aaggtcaggt
      601 cgattggtat gaagcctacg ccgacctgga tggcgagcgg accaagctgc aggtattctc
      661 gatgcgttcg atggccagcg gagcggccta tcatcgggct tacctgaggg cgacgcagca
      721 ggcgtttctg gaagcccacg agttggcgtt ccattacttt ggcggcgtat tccgtcggct
      781 gcgctacgac aacctgtcga gcgccgtaaa gaagatactg cgtggccagg agcgggagct
      841 aacggctcgc ttcattgcct tccgttcgca ctggcagtat caggccgatt tctgtacgcc
      901 gggcgaaggc cacgagaagg gcggagtgga aggtgaagtg ggctacttcc gccgcaacca
      961 ctgggttccg gtaccggtgg ccagggacct tgcggaactg aacgcgaagc tgctggctga
     1021 ctgccgtcag gatgagagcc gtgtcttgtc aggccgcgtg gagcatgttg gcatgctgct
     1081 gctcgccgag aagcaacacc tgcggccact tccagcggaa gacttcgagt tagcggaagt
     1141 cagcttcccg cgtgtcaacc aggcgggatg tgccaaggtg cgaaccaact tctactcggt
     1201 gccgatgcaa gcaggttcgg tggtcgaggc tcgggcttac tccagtgtgg ttgagttccg
     1261 gcaggacggg gtgcgagtgg cgcagcacca gcgtagctat gagcgggcac agcaagttct
     1321 ggatctcgaa cactatctgg aagtgctgga acgtaagccc ggtgcgttgc gcggatcgaa
     1381 gccgctggcg cagtggcggg cacagggccg ctggccggag agctatgacc agctatggga
     1441 gttaatgaac gagcggcatg gacgacagga gggaacgcga tccatgatcg cgctcatccg
     1501 catgggtcgc gagttcagct acgccaagct ggaaacctcg gtggcgcaag cgatgcagtt
     1561 gggctgtacc gatgtggcgg ccatccgtca tctccttatg accgatgaac tgcagcacgc
     1621 gaccgtaacc accgtcgaga tcggcgcact ggctgcctac gaacgtccac tgccgaccat
     1681 ggctgagtat aaccagctgc tctctgagcg cacgatcgag gtgcaggcat gagtctggaa
     1741 gccgcaagca tcgctcaaca ctgccagact ctacgactga ccgccatcgg cacgcagttc
     1801 ttatcgctgg ccgaagaagc cagtcagcac aaccactccc atcttcatta tctggaagcg
     1861 ctgctccaag ctgaggtcga ggaccgccaa cggcgctcga tcgaactgcg actcaaagat
     1921 gcgcatctgc ctcgaatgaa gacactggag gagttcgact tcgggcagtc gccaaacatc
     1981 cccgcctcgc gcattcgagc cctggccgaa ggtggctatc tggaacgctc cgagcccgtg
     2041 cttctggtcg gcgaaccggg gaccggaaaa acgcatctgg ccaccggtct ggccatcgcc
     2101 gcctgtcgcc agcggaggcg cgtgcgtttc acaaccgctg ccgccctggt caaccaactg
     2161 gtcgaggcac aacgcgaaca gagcctcagt cgcatgctgg cacgctggtc gcgggtggaa
     2221 ctgatcgtga tcgatgaact gggctatgtc ccactggccg aggttgccgc cgagctgctc
     2281 ttccaggtca tcgccgagcg ggcggagaaa gcggccatta tcgtgaccac caacctgccc
     2341 ttctccgaat ggccacaggt ctttaccaat gcccggctct gcaaggccgt gctcgaccgg
     2401 ctcaccgatc aggcacacat tatcgaaaca ggttcggagt cgtaccgctt tcgcagaacc
     2461 ttgcggaaga aacgcaaaga gtgatctctg gaggactcgc cgttccattc tgcttccgct
     2521 tcgccctccg ggctacgctc cagcagaacg gaactctacc ctcttaacct tcaacgtccc
     2581 aaggtgggcc agattaaacc gccgaagtgg gccaaaccaa cttgccaaac gcaattgaag
     2641 ccacttgtac ataaggcgaa gagtcaaaag acgcctctgt cgaattcgtc ggtgcaggcg
     2701 catcggcaga agctccactg aggctcgccg acccttctga agatgctctt atcgcggtct
     2761 gttgtacctg ttt
//
LOCUS       DASRKK010000559         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1123426, whole genome shotgun sequence.
ACCESSION   DASRKK010000559 DASRKK010000000
VERSION     DASRKK010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123426"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFC15_12875"
     CDS             <1..215
                     /locus_tag="VFC15_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11094.1"
                     /translation="TEDPGRCSDAPSSPVSDAKYRQKTESVFAKTFKSLLQQNRHIAD
                     IRGIATFCPLLDKSGQCAQQTPEFFG"
     gene            complement(296..1231)
                     /locus_tag="VFC15_12880"
     CDS             complement(296..1231)
                     /locus_tag="VFC15_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM11095.1"
                     /translation="MKAESDSLERILQEAAEHQAEIVATPIKANGHSVPTNRQVAASA
                     AIAWVDMSGWDDSPAPQREWAVKDRIPLRQAGLFSGEGGTGKSIIELMKNVAHVTGKD
                     WLGSMPEHGPAFYIGAEDEEDELRRRLESIAKHYGTNFGELIEGGLHIKCLLGQDATL
                     CAASGKSGRVEITGLYRQLYEAAGDIKPKNISIDTLSRAFAGNEIDRVQVYAFAMHMQ
                     ALAMVANGSVTVLSHPSLAGMASGSGISGSTAWHGAFRFRAYLRGVKADKDEQPESDL
                     RELEFKKKSIWPPWRDGLRSLGSGDIRPARCWQLT"
     gene            complement(1228..1476)
                     /locus_tag="VFC15_12885"
     CDS             complement(1228..1476)
                     /locus_tag="VFC15_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11096.1"
                     /translation="MKNERVAVLKKRLRALWRDVKAARGQCTDEIIAAKFCALAGKSG
                     LTAALGRYGEEDIAHVVSWALRGMNPFETGPLHGEPRE"
     gene            complement(1579..2333)
                     /locus_tag="VFC15_12890"
     CDS             complement(join(1579..2000,2000..2333))
                     /locus_tag="VFC15_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611908.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM11097.1"
                     /translation="MTELFLLSEAQMCRIEPFFPLSHGVPRVDDRRVISGIVFVIRNG
                     LRWRDAPREYGPHKTVYNRFVRWSRLGVFNKIFAELARKAGKPRRLMIDATHLKAHRT
                     AASLLKKGLFPRRIGRTKGGLNSKLHAVCDGHGRPLIMLLSEGQMSDYKGAALMLTAL
                     PKAKELLGDKGYDADWFRKALAERGITACIPSKSNRKKPIKHDRTLYRQRYKIENMFG
                     RLKDWRRVHTRYDRCAHTFMSAICIAAIVIFWL"
     gene            complement(2362..>2545)
                     /locus_tag="VFC15_12895"
     CDS             complement(2362..>2545)
                     /locus_tag="VFC15_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11098.1"
                     /translation="SRAFTSRSEDAQSSSVPSIDAPAIHDPPNHGRPWIQKSKENATV
                     VLGSGPIKYLADQMIG"
BASE COUNT          521 a          743 c          696 g          585 t
ORIGIN      
        1 gaacagagga ccctggtcgt tgttctgacg ccccatcatc gcccgtctcc gacgcaaaat
       61 atcgtcagaa gacggaatca gtgttcgcca aaaccttcaa atcacttttg caacaaaatc
      121 ggcacatagc ggacattcgc ggcatcgcaa cattttgtcc gttattggac aagagcggac
      181 aatgcgctca acagacgccg gaatttttcg gataacgaat tgggctgcca aagccggtag
      241 gcgcgctaag attttcaaaa ataggtcgtc aatcgtttgc tctcgggcca gggcttcaag
      301 tgagctgcca gcaccttgcg ggacgaatat cccccgatcc cagcgaacgc agaccgtctc
      361 gccaaggggg ccatattgat ttttttttga attccaattc ccgcaggtcg ctttcgggtt
      421 gttcgtcttt gtcggccttt accccgcgca ggtaggctcg aaaccgaaaa gcgccgtgcc
      481 aggcggtcga gccggaaata ccggacccgg acgccatgcc cgctaggctg gggtggctca
      541 ggaccgttac cgatccgttt gcgaccatcg ctagggcctg catgtgcatg gcgaaagcgt
      601 aaacctgcac ccggtcgatt tcatttcccg cgaacgcccg cgaaagggtg tcgattgaga
      661 tatttttcgg tttgatgtcc cctgcggctt catagagctg gcgatagagg cccgttatct
      721 cgactctgcc gcttttgcca ctagccgcac aaagcgtggc gtcctggcct aagaggcatt
      781 tgatatgaag gccgccctcg attagttccc cgaagttggt tccgtagtgc ttggctatcg
      841 actcaagcct gcggcgcagt tcgtcttctt cgtcctccgc gccgatatag aaagctggcc
      901 cgtgttccgg catagagcca agccaatctt tccccgtgac gtgagccacg tttttcatta
      961 gctcgataat gctttttccc gttccaccct cgccagaaaa aagcccggcc tgccgcagtg
     1021 gaatacgatc tttgactgcc cactcccttt gcggtgctgg ggaatcatcc cagcccgaca
     1081 tatcaaccca agcaatcgcg gcgctagcag ctacttgccg attagtaggg acgctgtgac
     1141 cgttagcctt gatcggggtc gcaacaatct cggcctggtg ctcggcggcc tcttgtagaa
     1201 ttcgctcaag gctgtcggat tcggccttca ttcgcggggc tccccgtgaa gcgggccagt
     1261 ctcaaaaggg ttcatgccgc gcaaagccca actcaccaca tgggcgatgt cctcctcgcc
     1321 atagcgccca agcgcagccg ttaatccgct tttgcctgcc aatgcgcaga acttggccgc
     1381 tattatttcg tccgtacact ggccgcgtgc ggccttaaca tcgcgccaca aggcccgcag
     1441 acgcttcttt aaaactgcaa cgcgctcgtt tttcatgccg tggccctcgt ccccggcagg
     1501 tcggcaaaca aaaagctgcg cgacattatc gcttgaacag catccgggcc gatattggct
     1561 agggtcagga ctcattgatt atagccagaa gatgacgatg gcggcgatac agattgcgga
     1621 catgaatgtg tgagcgcatc ggtcgtagcg tgtatgaaca cgtcgccagt ccttgagcct
     1681 gccgaacata ttctcgattt tatagcgttg gcggtagagc gtacggtcgt gtttgatcgg
     1741 ctttttccgg ttggattttg aagggatgca agctgtgatg cctcgctcgg cgagagcctt
     1801 gcgaaaccag tcggcatcat agcctttatc gccgagaagc tccttggcct tcggcagggc
     1861 cgttaacatc agagccgcgc ctttatagtc gctcatctgg ccttcgctca gcagcatgat
     1921 cagcggccgc ccgtggccat cgcacacggc atgcagtttc gagtttaagc cgcctttcgt
     1981 gcgcccgata cgtctgggaa cagacccttt tttaaaaggc tcgcggcggt gcgatgggct
     2041 ttcagatggg tcgcgtcgat catcagccgg cgcggcttgc cagccttgcg cgccagttcg
     2101 gcgaaaatct tgttgaacac gccaagacgg ctccagcgca cgaaccgatt gtagaccgtc
     2161 ttgtgcggac cgtattcgcg cggagcatcg cgccaccgca acccgttcct gatgacgaag
     2221 acgatcccgc taatcacccg tcggtcatca actctgggaa caccatgcga cagtgggaaa
     2281 aacggctcga tccggcacat ttgcgcctcg gagagcagga acaattcagt catggcgacg
     2341 cctccttgcg ccgccattga atcaaccgat catctgatcc gcaagatatt taatgggtcc
     2401 tgaccctagc actacagttg cattttcttt tgacttctga atccatgggc gaccatgatt
     2461 cggaggatcg tggatggcgg gagcatcgat cgaggggacg cttgagcttt gggcgtcttc
     2521 gctacgggat gtgaaggcgc ggctt
//
LOCUS       DASRKK010000560         8681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1319119, whole genome shotgun sequence.
ACCESSION   DASRKK010000560 DASRKK010000000
VERSION     DASRKK010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319119"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1315
                     /gene="queG"
                     /locus_tag="VFC15_12900"
     CDS             221..1315
                     /gene="queG"
                     /locus_tag="VFC15_12900"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZM11099.1"
                     /translation="MPSHLHLSSEIKRLATDAGFDLAGVAGVNDTSEHQFFPQWIANG
                     QAGDMKYMEARNDAGNLKRASLANAAPWARSVIVCALNYNTDQPYSTHAGTTSQAWIS
                     RYAWTQRDYHDVVLAKLRQLESRFVALCEQDGSGTPRTWCYVDTGPVIERIFAKYAGI
                     GWIGKNTCILNQDLGSWLFLGVMLTSLELPPDLPPADRCGSCTRCLDACPTNAFPAPY
                     QLDATKCISYLTIEERGTVPEELRSGIGRQVFGCDICQDVCPWNREAPLTKTPELSAR
                     QQLVNPDLEWLARLSPEEFRDVFRGTPVKRAKHNGLLRNVAIAIGNSGEDRFLPVLED
                     LIRHDDPTIAEAANWALRRILSRSNSAPEA"
     gene            1680..2177
                     /locus_tag="VFC15_12905"
     CDS             1680..2177
                     /locus_tag="VFC15_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM11100.1"
                     /translation="MTEALSRRLKQARFANPQQEAALSLAVAAGTMHDLIDGICKHYR
                     LTRPQYNVLRILRGVHPKGHPRCEIAQRMVERAPDITRLVDRLQARGLVRRTRGGDDQ
                     RQAITHITAKGLKLLHAMQPELEAQTTARLSHLTDKDCHELSRLCTVIFENDACAPDP
                     CADSH"
     gene            2293..2931
                     /locus_tag="VFC15_12910"
     CDS             2293..2931
                     /locus_tag="VFC15_12910"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZM11101.1"
                     /translation="MNTLLIVNSSPRSNSTSRRLTRQFSEDWKTRNPSGRIVERDLSD
                     GTIPYLSEPWIEAAYVPEPERTPAQRDLLSLSDTLIDELLAADVILLGIPMHNFSVPA
                     SFKAWIDQIARAGKTFSYTSQGPKGLIPSHKKVVAVLSRGGIYAPDSPQGAMDFQVSY
                     LRQVLALVGLTDVTFVHADRQAMGGQAAQLATERAIQQVSSVVDSFATKLVA"
     gene            complement(3072..3275)
                     /locus_tag="VFC15_12915"
     CDS             complement(3072..3275)
                     /locus_tag="VFC15_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZM11102.1"
                     /translation="MKEKGTVKWFNGAKGYGFIQRSTGEDVFVHFSAIQEGGYRSLNE
                     GETVEFELTKGPKGLQAANVARV"
     gene            3794..4738
                     /locus_tag="VFC15_12920"
     CDS             3794..4738
                     /locus_tag="VFC15_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZM11103.1"
                     /translation="MQATALQTGTAAKRMLAQDDSRQVFQQEAGTGDGLFDRLRRYGW
                     PTLRYLTQTEVHTYAFSVAAYSILSLFPVIVLLLTVVRRVFRSQQMYDVVLQLLRDFL
                     PSNQDFVIRNLQFLASVHGRGQVLSLIMLFVTSTGVFLPLEVALNSIWGFTKNRSYLM
                     NALVSVGLASACGTLALLSVYLTAKNLRILGLAIGDQNFVFRGLGFVVMKAFVIATTI
                     AIYFLIYWLLPNGKVPVRAVLPAAIVTGLLSEAAKYLYVLLLPWLNFQEVYGPFSVSV
                     TLIFWSFCSGMLLLGGAYFSAAQHSDRVEAQRETAGAR"
     gene            complement(5283..5960)
                     /locus_tag="VFC15_12925"
     CDS             complement(5283..5960)
                     /locus_tag="VFC15_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11104.1"
                     /translation="MKKISTSSAVAVFSILLLTAQARDVGPKNQIGELEISPTGVQIT
                     TTSGRSKATAGFHYVRFGLKVKNIGNYAICAMLSADLQNTLNVGGGDGVIEFKAKNPA
                     EVARGSSIRQLLPGEELVGSIVFSDLRDGVEPVTLTIDGGTNQGCSREDPESIEIRDQ
                     RVSFAVSEIPRESSARDAQTGWPEAVAGIKNSFVSCYARERGIQHDGEIIGKISWPLA
                     LFTKRLT"
     gene            complement(5981..8092)
                     /locus_tag="VFC15_12930"
     CDS             complement(5981..8092)
                     /locus_tag="VFC15_12930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM11105.1"
                     /translation="MLRRILFFLLVCSLFGSAQTKHPFTFEDMMQLKRVGEPAVSPDG
                     KWVGFSAVDVNLDANTKTPHLWIIPVAGGESKRLTPASGAGEDRVRFSPDGKRVIFES
                     DRGGGTQIWVQDFDAAAGALTGEAKKVTTISTEASGGTWSPDGKSVLFVSSVFPDCND
                     DACNKQRDEERAKSKVKARTYDKLMFRHWNHYYDGKKSHLFIVPIEGGVARDLTPGAY
                     DVPPFSLGGQDQYNFSPDGTEVAYTCNTDESEATSTNSDVFVIPVGGGSAKRITTNPG
                     SDSTPIYAPDGKYIAYRSQLRGGYESDRFRLMLYERATGKITDLAPNFDRWVDAIAWS
                     PDSKTIYLTAGNEGRSPIYQVAVGGENPWPQELVDGYNDSPTPTPDGKTLVFGTMSIS
                     APNEIYTAPLMFAQCRMAFPPNAYSVPDCLMSKAKPLTHMNDEVLSKVGMEPMESFWF
                     AGAAKEKVQGFLIRPPGFDHNKKYPVKFLIHGGPQGAWGDDWSYRWNPELFAANGYVV
                     VMVNFHGSTGYGQKFIDAINGNWGGAPFDDLMLGLDYAEKEYPFIDKGRECALGASYG
                     GYAINWIEGHTDRFKCLVSHDGMFNTESAFGTTEELWFNEWEFKGTPWTDREMYRKWS
                     PHLAAANFMTPMLVVHGQLDYRLDVSEGFQMFTTLQRLKVPAKLLYFPDEGHWVLKPQ
                     NGRFWYQTVNAWVDEWTKAAK"
BASE COUNT         1807 a         2704 c         2300 g         1870 t
ORIGIN      
        1 gcgaccatct ttccagtcat ccccgcgttc tatttccgcc ccacctccac cgacgagatc
       61 gcccacgagt acgtgaaccg cgtgttggcg cacgtcggac tgccgcagcc cgacgcctac
      121 cagtggaaag cgtagccatc acgttcagag tggtagggcc gcgagcgcgg ctacgatcca
      181 tcccgcccgt ataatcagcg cgaagccccg gtagatcaac gtgcccagcc atctccatct
      241 cagcagcgaa atcaagcgac tcgccacgga cgccgggttc gacctcgccg gagtcgccgg
      301 tgtcaacgat acctcggaac accagttctt tccccaatgg atcgccaacg gccaagcggg
      361 cgacatgaag tacatggaag ctcgcaacga cgcgggaaac ttgaagcggg cctctctggc
      421 caacgcagcg ccttgggctc gatcggtcat agtctgtgcg ctgaactaca acaccgacca
      481 gccttactcc acccatgctg gaacgacctc gcaggcatgg atctcccgct acgcctggac
      541 ccaacgcgac taccatgacg tggttctcgc caagttgcgc cagcttgaat cccgattcgt
      601 agccctttgc gagcaggatg gaagcggcac tcctcgcacc tggtgctacg tggacacggg
      661 ccccgtcatc gagcgcatct tcgccaagta cgccggcatc ggctggatcg gcaagaacac
      721 ctgcattctt aaccaagatc tcggatcgtg gctgtttctc ggcgtcatgc taacgtcact
      781 tgaattaccc cccgaccttc cgcctgcgga tcgctgcggc tcctgcacgc gatgcctcga
      841 tgcctgcccc accaacgcct tccccgcccc ttatcaactc gacgcgacca agtgcatctc
      901 gtatctcacc atcgaagagc gcggcaccgt tcccgaagag cttcgctcag gaatcggtcg
      961 gcaagtattc ggctgcgaca tttgccagga cgtttgccct tggaaccgcg aagctcctct
     1021 gacgaagacg ccagaactct cggcgcgcca acagttggtg aatccggacc tcgaatggtt
     1081 ggcacgcctc agcccggagg agtttcgcga cgtgtttcgt ggcacgccgg taaagcgcgc
     1141 caaacataac ggactgcttc gcaacgtcgc aatagccata ggcaacagcg gagaggatcg
     1201 atttctccct gttctcgaag atctcatccg ccatgacgac ccaacgattg cggaagccgc
     1261 aaactgggcg ctgcgacgaa ttctgtcgag atcaaattcg gcccctgaag catgaatcct
     1321 cattctcgat caaaggccgg acatgctcga cggcgtgtag agattacccg ccactcaaac
     1381 ctgtactacc tctataccga gcgaaactca cgttttgaac tgtggcacgt ctattgcttc
     1441 acggaatagc aacgaacaaa ttcgagtttc cgtggaaggt gtgggtcccc gcccatgcct
     1501 cctgtggatg atgcccaacc gcacccaact cggggttgaa gttccccggg gcatcaggcc
     1561 ctccggcgtc ctccggcacg gagggcaatt tttcgcccgc ggggcacgcc gcacaaccat
     1621 cacggcagac gctgcaataa cgtgtgacaa cgcgctgatt tctggctata acagacacca
     1681 tgaccgaagc tcttagtcgg cgattgaaac aagcccgctt cgccaatccc cagcaggaag
     1741 ccgcactcag tctggcagtg gctgccggta cgatgcacga tctgatcgac gggatttgta
     1801 agcactatcg tcttacccgg ccgcagtata acgtcctgcg aatcctccgg ggagtgcatc
     1861 ccaaggggca tcctcgctgc gaaatcgccc agcgcatggt cgaacgcgct cccgacatca
     1921 ctcgacttgt ggaccgcctt caagcccggg gcctggtgcg acgcactcgc ggtggcgacg
     1981 atcagcgcca ggccataacc cacatcactg ccaagggact taagttgctg cacgcaatgc
     2041 agcccgaact cgaagctcag accaccgccc ggctctccca tctcaccgac aaggattgcc
     2101 atgaactcag ccgcttgtgc actgtaatct tcgagaacga cgcttgcgct ccagacccgt
     2161 gcgcggattc ccactaaccg atcggccctc ccccgttgca tccttctccg ggtttccggc
     2221 atcttatgtg ttgtaacaca tatttaggcg ttgccacatg gattggcggt gaacgccacc
     2281 ggagatgccc tcatgaatac cctgctgatc gtgaattcca gcccccgcag caattccacc
     2341 tcgcgccggc tgacccgcca gttctctgaa gattggaaga cccggaaccc cagtggccgg
     2401 atcgtcgagc gcgacctgtc agacgggacg attccctacc tgagcgagcc gtggatcgaa
     2461 gccgcctatg tgcccgaacc ggagcgcaca cctgcgcaac gcgatctgct gtcactctca
     2521 gacacgctga tcgacgagct tttggctgct gatgtgatcc tgctgggcat tccgatgcac
     2581 aacttctccg tgcccgcgtc attcaaagca tggatcgacc agattgctcg cgccggaaaa
     2641 accttcagct acaccagcca gggcccgaag ggtttaattc cttcgcacaa gaaggtggtc
     2701 gcggttctgt cgcgaggtgg catatacgca cctgacagcc cgcagggggc gatggacttc
     2761 caagtctcgt acctgcgaca ggtgcttgct ctggtggggc ttacggatgt cacgttcgtc
     2821 cacgcagatc ggcaggccat gggcgggcaa gcagcgcaat tggctaccga acgggcgatt
     2881 cagcaggtat cgtcggtcgt cgacagcttc gcaaccaagc ttgtggcttg agtcactgct
     2941 aacattgctc ccagaaagtt aaaccctccc ttgcgggagg gttcggtggt tatgtcgcta
     3001 actcggaaga ccgccgggcg catcgaaatg gcgcggcgcg ccaccctgat cttccccttg
     3061 gcggcagatg cctagacgcg cgcgacgtta gcggcttgca gccccttggg gcccttagtc
     3121 aactcgaact ccacggtttc gccctcattc aggctccggt acccgccctc ctgaatagca
     3181 gagaaatgta caaaaacgtc ctcgccggta gagcgctgaa taaatccgta gcccttggcc
     3241 ccgttgaacc acttgactgt acctttttcc ttcacaactt actcctttcg tatgttcgtc
     3301 ctgagataac ccctgcttcg gggatgttcc gcacgcgacc ctgaggcgtg tacgaaagac
     3361 ctccctacgt tgccgggaaa cagggttgcc gctcaaatcg cagcttttac gcaatgaaaa
     3421 aagcccacgc atctgcccga gcgcaccgat acgtttgcgt cccggcagtt acgtgaagcc
     3481 tgtgcaatgc gagaatttcg actgctgacc gaaacatcaa cccaaatccg ccctttcaaa
     3541 ggtggagcta cgaataatta caggaactcc caattttggc aagtgtttct gtcacagtgc
     3601 tttgagtgcc atttgaagcg gagtgtacgc acgacgcatg tctcccactc aatacccggt
     3661 cgtctccgtt tggtaccatg gactgtagat gagcagtaaa ctcgaatctc cggccggtcg
     3721 acctcagtta ccgtcccaat cgtcctcccc gcagactcct ccatcgaacg ttaaaccgct
     3781 tgggaagaat gacttgcagg caactgctct gcaaaccggc accgccgcaa agcgcatgct
     3841 ggcgcaagac gattcccggc aagtatttca gcaagaagcc ggcacaggcg acggtctgtt
     3901 cgatcgcctg cggcgctacg gctggcccac acttcgctac ctgactcaga ccgaagtcca
     3961 cacctatgcc ttctcggtcg cagcgtattc gatactttcg ctttttcccg tgattgtctt
     4021 gctgctcacc gtcgtccgcc gcgtctttcg ctcccagcag atgtacgacg tagtactgca
     4081 acttctacgc gacttcctgc ccagcaacca ggactttgtg attcgcaacc tgcagtttct
     4141 cgccagcgtg cacggtcgcg gacaggtgct gtcactgatc atgcttttcg tcacttccac
     4201 cggcgtcttt cttcccctcg aagtagcgct caacagcatt tggggattca ccaagaaccg
     4261 ctcgtacttg atgaacgcgt tggtttctgt gggactcgcg tcggcttgcg gaacgctggc
     4321 actcctctcc gtctatctca ctgccaagaa cctcaggata ttgggacttg ctatcggtga
     4381 ccagaacttt gtttttcgcg gcctgggatt cgtggtgatg aaagcgttcg tgatcgctac
     4441 caccatcgcc atctatttcc tcatctactg gctgctgccc aacgggaaag ttcccgtacg
     4501 cgccgtactg cccgcggcga tcgtcaccgg attgttgtcg gaagccgcga agtaccttta
     4561 cgtactgctg ctgccttggc tgaactttca ggaagtctac gggccgttct cagtgtcggt
     4621 cacgctgatc ttctggtcgt tctgcagcgg catgcttctg cttggcggag cctacttctc
     4681 agccgctcaa cacagcgacc gcgtagaagc acagcgcgaa acagcgggcg ctcgctaaga
     4741 aagctcattg gagacggcag gaatgtaggc tcgtcgagga atggctgaaa tccccgtagt
     4801 tctttagcgc tgcgcccaag cggtgtttgt gatttttgga aactctgcga cgcaaatctc
     4861 gatactcttt caccgtctct ggagagtctt gccagactag ctctccagca atcatgcggg
     4921 acacgcggcc gcgtaagaca tcgactcctg cccaggactc aagcagatcc ggccggtaca
     4981 agagccacac aatgaagccc tcccagtaat tcctctcggg ctttgccagt ccatcccaag
     5041 tccggtcgac caaatcatgc ccgtccgatg ccattcgttc ttctgcggcc tcaagtccaa
     5101 aatgctcgcc taccgcccgt atttcctccc tgtgcagcgg tcgaaatggc tcatagaaag
     5161 tccagcattc accgaagcgc tgataccaag ggtggccgaa ttctcgatga cgcaaccctg
     5221 gcaaccgctg cgcgtgttcg tcttttgctg atgaaggtgc cggcacaccc tgccaagcat
     5281 aatcaggtca atcgtttcgt gaacaaagcc agcggccatg aaatcttccc gattatttct
     5341 ccatcatgtt gaatgcctcg ttcgcgggcg tagcatgaca caaatgagtt cttgattccg
     5401 gctacggctt ccggccagcc ggtttgggcg tcacgggcgg aggattcccg tggaatttca
     5461 ctaaccgcaa aactcacgcg ctggtctctt atctcgatcg actcgggatc ttctctgctg
     5521 cacccctgat ttgtcccgcc gtcgatcgtg agggttactg gttccactcc atccctcaaa
     5581 tccgagaata caatactccc aaccaattct tcgcctggaa gcaattggcg aatggagctt
     5641 ccacgggcga cctccgctgg gttcttggcc ttaaactcga tcacgccatc acctccgcca
     5701 acgttaagcg tattttgcaa atcagctgaa agcatggcac aaatcgcata gttgccgata
     5761 tttttgacct ttaggccgaa cctcacgtaa tgaaaaccgg cagtagcttt ggaacggcca
     5821 ctcgtcgtag tgatctggac gccggtgggg gatatttcca gttcacctat ctggtttttg
     5881 ggaccgacat cgcgagcttg cgcagtgagc agcaaaatgg agaaaacggc aacggcagat
     5941 gacgtactga tcttcttcat cagttacctc ccgactccct ctattttgcg gccttggtcc
     6001 actcatccac ccaggcattc acagtctgat accagaagcg gccgttttgc ggcttcaata
     6061 cccagtgccc ttcgtccgga aagtacagca gcttcgccgg cactttgagc cgctgcagcg
     6121 tggtgaacat ctggaagcct tccgaaacat caagccggta atcgagctgc ccgtgtacca
     6181 ccaacatagg cgtcataaaa ttggccgccg ccaggtgcgg agaccacttg cgatacatct
     6241 cgcgatccgt ccacggcgtg cccttgaact cccactcgtt gaaccacagc tcttcggtgg
     6301 tcccgaacgc cgactcggtg ttgaacatgc catcgtgcga gaccaggcac ttgaagcggt
     6361 cggtgtggcc ctcgatccag ttgatcgcgt agccgccata actggcgccg agagcgcact
     6421 cgcggccttt gtcgatgaat ggatactctt tctccgcata gtcgagaccc aacatgaggt
     6481 catcgaaggg agcgccgccc cagttgccgt tgatcgcatc gatgaacttc tgtccgtagc
     6541 cggtcgagcc gtggaagttg accataacaa ccacgtatcc attggccgca aatagctccg
     6601 gattccagcg gtacgaccag tcatcgcccc aggcgccttg cggaccgcca tgaatcaaga
     6661 atttcaccgg atacttcttg ttgtggtcga agcccggcgg ccggatgaga aagccttgga
     6721 ccttttcttt cgctgctccg gcgaaccaga acgactccat cggttccatc ccaactttgg
     6781 acagaacctc atcgttcata tgcgtcagcg gcttggcttt tgacatcaag cagtcgggaa
     6841 ccgaatacgc gttcggcgga aaagccatgc ggcactgtgc gaacatcagc ggcgctgtgt
     6901 aaatctcgtt gggcgccgag atcgacatgg tgccgaacac cagggtcttc ccgtccggtg
     6961 tcggcgttgg actgtcgttg tagccatcga ccaattcctg tggccacgga ttctcgccac
     7021 ccaccgcaac ctggtatatc ggcgaacgcc cttcatttcc agccgtcagg taaatcgtct
     7081 tcgaatcggg agaccacgcg atcgcatcca cccagcgatc gaaattcggc gccaagtccg
     7141 tgatcttgcc ggttgcgcgc tcatacagca tcaggcggaa gcgatcgctc tcgtagccgc
     7201 cgcgtagctg cgagcgatag gcaatgtact tgccatccgg agcgtaaatc ggcgtgctgt
     7261 cgctcccggg attggtcgtg attcgcttcg ctgaaccgcc gccgactggg atgacaaaga
     7321 catcagagtt ggtgctggtg gcctcggact cgtcagtgtt gcaggtgtaa gcgacttccg
     7381 tcccgtcggg cgagaagttg tactggtcct gtcctcccaa gctgaacggc ggcacgtcgt
     7441 aagcgccggg cgtcagatcg cgcgccactc cgccttcgat gggcacgatg aacaggtgac
     7501 tcttcttgcc atcgtaatag tggttccagt ggcggaacat gagcttgtcg taggtacggg
     7561 ccttcacctt cgacttggcc cgctcttcgt cgcgctgctt gttgcaggca tcgtcgttgc
     7621 agtccggaaa gaccgacgaa acaaacagca cactctttcc atccggcgac cacgtcccgc
     7681 ccgaagcctc ggtcgagatc gtggtgactt ttttcgcttc acccgtaagc gcaccagccg
     7741 ccgcatcgaa gtcctgcacc caaatttgag tgccgccgcc gcgatcggac tcaaagatca
     7801 cgcgcttgcc atcgggcgag aaacgcaccc ggtcttcgcc cgcgccgctg gccggagtca
     7861 gcctcttcga ctcacccccg gcgacgggga tgatccacag atgcggcgtc ttggtgttag
     7921 cgtcgaggtt cacatcgaca gcactgaagc caacccactt gccgtccggg gaaaccgctg
     7981 gctcgccaac tcgcttgagt tgcatcatgt cttcgaaggt gaaggggtgt ttggtttggg
     8041 ctgatccaaa cagcgaacag acgagcagga agaaaagaat gcgacgcagc atgatgcctc
     8101 cgaggcgggg ccgggagtcc cgcgcaaact gtctagtcta aatcaccgcg atacagggga
     8161 gagggaattt caccccgtgg agcacggaga aaccggcggg tgatcaccac agaggcacag
     8221 agatacagag gaatcgggat ttcaccacgg cgaacacgga agacactgag aattgggatc
     8281 agttaacgcg ttggtcgggc tgcccgtccg ttcgggataa cttgccagat ggcgtgttat
     8341 cgccagctac ctccgaggag ccaacttccg tcgagtctgg catccttcct gagtggttgg
     8401 cgagcaccac tgaattactt cgaagagccg tatggatcca gtcgacgata ctcggatggg
     8461 acctaatcgc cgcaaagctt gttaatttca aaagtcgtct gttttttctt ttatgatctc
     8521 gctcaccatt gtgtaaaatc ggttctgagc aagaatctga acagccccgc tattctagcg
     8581 gttcgtctgc tttggccttt atcgtacccg cagccgccgt cgctgggtaa atgtgggaac
     8641 cagcgttttg tgcgggtttc caagcgttgt gggaatcggg g
//
LOCUS       DASRKK010000561         8475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_342109, whole genome shotgun sequence.
ACCESSION   DASRKK010000561 DASRKK010000000
VERSION     DASRKK010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342109"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFC15_12935"
     CDS             <1..984
                     /locus_tag="VFC15_12935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM11106.1"
                     /translation="DWLGARILLIAGLAIGATAFTIAGLVDSFWVMVAMFALAGVGNT
                     VYHPADYALLSHHVPSDRIGQAFSVHTFAGMLGSAVAPASLLMMQSLWGWRGAFIGAG
                     LLGFAVAALLLAVRETPETKAPAPAPREADATAAVGWRLLLSPAILLNLVFFVLLAMM
                     SGGTYNYSVVALGALYGTPVTVANAALSANLLLSAIGVLIGGLLVGRTKRHGLVATLG
                     LAGIALFIALVAQFDLGSLALIAAMAAAGFCSGVIMPSRDMIVREVTPPGSFGKVFGF
                     VTTGFNIGGIISPLMFGAIMDHGSPKLVLVLVAAFSLIAIVTVASRPRRAA"
     gene            1100..1173
                     /locus_tag="VFC15_12940"
     tRNA            1100..1173
                     /locus_tag="VFC15_12940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1132..1134,aa:Gln,seq:ctg)
     gene            1532..1855
                     /locus_tag="VFC15_12945"
     CDS             1532..1855
                     /locus_tag="VFC15_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11107.1"
                     /translation="MCTQKLLNRPLAWRFPWLNKHSGSGSDWIYVLNSDLAASREWLH
                     IGKMAPSSKKTASYRKRAKDINKRLKERASTADRVKLTKKQKALSDMADNEDWLAGKP
                     GSQLK"
     gene            2247..2834
                     /locus_tag="VFC15_12950"
     CDS             2247..2834
                     /locus_tag="VFC15_12950"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZM11108.1"
                     /translation="MNSNDLRMMARCVELSRHATSQRELPFACVICKDGEIVVEATNR
                     VKRDADVTRHAEMVAISEAQRKLGKKKLSRCTLYSNVEPCAMCSYCIRETRISRVVYA
                     IRSPIMGGFSKWKLLQDKELSAVIPEIFGKAPLVIGGVLEREAEAVWHDWHPLIWRII
                     KSRGCFSGIPPQPSGRDAETPRRGSFFRHLLTFHH"
     gene            3311..3571
                     /locus_tag="VFC15_12955"
     CDS             3311..3571
                     /locus_tag="VFC15_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11109.1"
                     /translation="MPCHLAPQKLPPSVAPFILRGECLYGIDSVMCPLDRRREAWKRL
                     ETELDRPKLAAMTREIDLSGVAEAGVAILACQVRGRIVVKIG"
     gene            3673..4131
                     /locus_tag="VFC15_12960"
     CDS             3673..4131
                     /locus_tag="VFC15_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11110.1"
                     /translation="MLRGFALLTGLLATVPAMAGEMSADEARRFVIGTMFNYTCFEGT
                     RGQGRVLSDGSVVGSIQVRGSGQVRYAQLPPGTLRVKGEAVCASLRGLPVEPCFNLER
                     TSDNSFRGSISGLGFASCEFNRHNARTTVMHSARSTQPLGLRPSLTANNN"
     gene            complement(4144..4337)
                     /locus_tag="VFC15_12965"
                     /pseudo
     CDS             complement(4144..4337)
                     /locus_tag="VFC15_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4468..4830
                     /locus_tag="VFC15_12970"
     CDS             4468..4830
                     /locus_tag="VFC15_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11111.1"
                     /translation="MPDWSKVSLAAQIGANIAYPVACAAGCLFLLATCLRFSRNHIKI
                     LDNLSANAYAMYLVHYIFVVCLQYALLGSNLFPIAKVAIVLSCTQILNWASSTAFNRF
                     ALGPRRCASQGAIGPVPH"
     gene            4859..5986
                     /locus_tag="VFC15_12975"
     CDS             4859..5986
                     /locus_tag="VFC15_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM11112.1"
                     /translation="MPLNLTLASRNLFHDRLRFVATMVGIVFSVVLVMVQMGLYLGFG
                     RMVTTVIDHTSADLWVMRHGTKCFEDPSLLSASKQTRVAATAGVAEAIPLVIGFSGWI
                     LPSGEITPVFVVGSDLQTLKPWNVTEGTVEALSAPGAVAVDRSYFERLGVSGIGSKAD
                     IRGHPVSVAAVTDGIRSFTTTPYIFSEMSRARTYTGIPSTMASYMLVKLSPGADLEHV
                     RRDLQASLPDSDVLTSAEFRERSRSYWLFGTGAGAALFAGAILGIIVGTVIVGQTLYS
                     STKDHLDEFATLRAMGSSHGYIYSVIIYQALLNAVIGFGLATLIGFAVVRFTMSSALP
                     VLLTPPLILAMLALTVIMCVASALAAIFRVLRVDPVMVFTR"
     gene            5983..6723
                     /locus_tag="VFC15_12980"
     CDS             5983..6723
                     /locus_tag="VFC15_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM11113.1"
                     /translation="MSVSVLEAKDVTTVLGRGAAQVHALRDVNLSLRGGELTVLMGPS
                     GSGKTTLLSVLGCMLAPTSGTVRVCGQTADGTDAEALAKLRRDHVGFVFQSYHLFPTL
                     TAAENVQVALDVRGIHGEEAQAKSRAALERVGLAHKTASYPLALSGGEQQRIAIARAI
                     VSDPSAILADEPTAALDAGNGQAIMRILAEIAKDPARGVLVVTHDSRVMSFADRIIHI
                     EDGRLVGDELGSADAAKMRTEPLSGRRA"
     gene            6945..7205
                     /locus_tag="VFC15_12985"
     CDS             6945..7205
                     /locus_tag="VFC15_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11114.1"
                     /translation="MYARTGAALLFILMTGAAALAANERSPNSSTTQSQGTEDEQSAC
                     TSDAMKFCDEDIPNTFAVLACLQAHRKRLTKACRHVLDSNGQ"
     gene            complement(8257..>8475)
                     /locus_tag="VFC15_12990"
     CDS             complement(8257..>8475)
                     /locus_tag="VFC15_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11115.1"
                     /translation="SLSANAYGIYLVHYVFAIWLQYALLDTGLLAIGKAAIVFGGTLI
                     MSWAITAGLGTVPFVSKLVTAKRGVRGA"
BASE COUNT         1665 a         2453 c         2513 g         1844 t
ORIGIN      
        1 gattggctgg gcgcccgcat cctattgatc gccgggctgg cgatcggggc aactgcgttc
       61 acaatcgccg gactggtcga ttcattctgg gtgatggtgg cgatgtttgc gctcgccggc
      121 gtcggcaaca cggtttatca cccggccgac tatgcgctgc tgtcgcacca tgttccgtcc
      181 gaccgcatcg gccaggcctt ttcggtgcac accttcgccg gcatgctggg atcggcggtg
      241 gcgccggcga gcctgctgat gatgcaaagc ctgtggggat ggcgaggcgc cttcatcggc
      301 gctggcctgc tcggcttcgc ggtggcggcg ctgctgttgg cggtgcgcga aaccccggaa
      361 acgaaggcgc ccgcaccggc gccgcgcgag gcggatgcca ctgccgcggt cggctggcgc
      421 cttttgcttt cgccggcgat cctgctcaat ctcgttttct tcgtgctgct cgcgatgatg
      481 agcggcggca cgtacaatta ttccgtggtc gcactcggcg cgctttatgg aacaccggtg
      541 accgtcgcca acgcagcgct gtccgccaat ctgctgctgt cggcgatcgg cgtgctgatc
      601 ggcggcctgc tggtcgggcg caccaaacgc catggactgg tggcaaccct tggtcttgcc
      661 gggatcgcgc tgttcatcgc gctggtcgcg caattcgacc tcggctcgct ggcgctgatc
      721 gcggcgatgg cggcggccgg cttctgctcg ggcgtcatca tgccgtcgcg tgatatgatc
      781 gtgcgcgagg tcaccccgcc cggctcgttc ggcaaggtat ttggcttcgt caccaccggc
      841 ttcaatatcg gcggcattat ttcgccgctg atgttcggcg ccatcatgga ccatggcagc
      901 ccgaagctgg tgttggtgct ggtggctgca ttcagcctga tcgccatcgt gacggtggcg
      961 agccggccgc gccgggcggc ctgataccga cgcttcgtgc gtgcgggcgg acttgtcaaa
     1021 tcggccgaat tgccccatgt caggggcctc cggcagatag gcggcggatc atttcgaccg
     1081 ccgacgcgtc cggccttgct gggggatagt tcaacggtag aacatcggac tctgactccg
     1141 agaatcttgg ttcgaatcca ggtcccccag ccaaccagtc ctgtctcccg cctacggctt
     1201 cgagacatgc cgaagaagcc cacattggtg ggggcttttg ctgttttaat cccgtctcca
     1261 ttcaccgaat ttggcaccgt tttggcaaat agacccgcct gtctccgtgg cttgtttttg
     1321 actaacggtt tgcgggggcc aacctgaatg gaaagcactg tctggtactg gcctgttttg
     1381 acccctagtg gccggttttg aaaatacagt ggcctatttt cataattgtt cgattttgac
     1441 tcacagcacg aaatgattca tgcatgtgca ttagggcgcg cttcgaatcc gaagctaaaa
     1501 gctggaatct catactttag aagttcaggc tatgtgcacg caaaaattgt tgaaccgccc
     1561 gctcgcttgg cggttccctt ggctgaataa acattcgggt agtggatcag attggattta
     1621 tgttctaaat tcggacctag cagcgtcgcg cgaatggctg catattggca agatggcacc
     1681 atcatccaaa aaaactgcaa gctataggaa gcgcgcgaaa gacattaata aacgcttgaa
     1741 ggagcgcgcc tcgaccgcag atcgcgtaaa gctaaccaag aagcaaaaag ctctaagcga
     1801 catggccgac aacgaagatt ggctggcggg caagcctggg tcgcagttga aatgaccgac
     1861 cacagcaacg cccccgccaa ctcgccaagc tcatcgtcga tattgcggcg ggaggtctac
     1921 ccggatgtgg ggatatcgca gcagagatga aagacgaatg ccacaaccag gagagcttct
     1981 ttagcttgaa ataggcgcaa gtcggcatcg ggcactggcg cgagcactgc aagacaacca
     2041 aaccgcacac gtcgcccgga tatcgaccgc ctgcgccaca gacgataaaa ccgttcctga
     2101 caccgccaga tcaggccgcg ccaatgcaat aatctttcca tcacgctggt acaaaatctg
     2161 tcgggccagc acaaataatc cgtcaggcca cattctcacg ctcggcattc aagctatttg
     2221 ggaaatatca gatgaatgca tcagtcatga atagcaacga cttaaggatg atggcgcgct
     2281 gtgttgagct ctcgcgacac gcaacgagcc aaagagagct tccattcgca tgcgtgatct
     2341 gcaaggacgg cgaaattgtc gtcgaagcga caaaccgagt taaacgagac gcagatgtga
     2401 ctaggcacgc agaaatggtc gccatttcag aagcgcagag gaaacttggg aaaaagaaac
     2461 tctctcgttg cacgctttat tcgaacgtgg agccgtgcgc catgtgttcc tactgcattc
     2521 gggagacccg cataagtcgg gtcgtttatg ccattagatc tccgatcatg ggcggattct
     2581 ctaaatggaa actccttcaa gacaaagagc tttcagccgt cattccggag atttttggta
     2641 aagctccctt agtcataggt ggagttcttg aacgcgaagc cgaagccgtc tggcacgact
     2701 ggcatccgct gatctggaga atcataaaat ctcgcggctg cttcagtggc atcccgcccc
     2761 aaccaagcgg ccgagatgct gagacaccac gtcgtggtag cttcttccgc catcttttaa
     2821 cgttccatca ctagcaacgc ggataaaatg aatagttgaa attttccaat ttctgtatct
     2881 cagccgtgtc cgtcccgaaa ctcatatccg cacgtagcga tgatgcagtc ccgacagaac
     2941 agggcttagc ggcaatgatt ccgtctcttt ggaccgctcg acccattggg gcgtccttgt
     3001 gcagcgataa gtgcgtgcgc gattcattat aatattagca agcgtacatg gtcagagttt
     3061 gccgcaggtg tggctcgcca aagatcagca tgcgatccag gcactcgcgg cgcacagtac
     3121 cgatcagacg ctccacgtga ccatttcgcc acggtgaccg gggtgagacc gaccggtcgc
     3181 ggatacctgg ttctcactcg gcacgtaaag acatgcccgt aggcacgatc attgtcgcgc
     3241 accagatagg actgcgctaa tccccaggtg aaggcatcgg taatctattg ggcagccact
     3301 cggccgtcgg atgccgtgtc acctcgcacc acagaagctg ccgccctcgg tcgccccgtt
     3361 cattttgcgc ggggagtgtc tttacggcat cgactcggtc atgtgtccgc tagatcgccg
     3421 cagagaggcg tggaaaagac tggaaaccga gcttgaccgc cccaaactcg ccgctatgac
     3481 ccgtgaaata gacctttccg gggtggccga ggctggcgtg gccatcctgg cctgccaggt
     3541 ccgtggccgg atcgtggtca aaatcgggta aatacgttta ggatttgtcg acgaacggcg
     3601 tggcatagtc cgctaggttt taaaaaccgg ccttggtttt gttaaattgg ttgcgtcagt
     3661 cggagttgcg tgatgttgcg tggatttgcg ttgctgaccg gcctgttggc caccgtgccg
     3721 gcgatggccg gcgagatgtc cgcggacgaa gctcgccgct tcgtcatcgg cacgatgttc
     3781 aactacacct gtttcgaagg cacgcgcggc caaggccgcg tcttgtccga cggctcggtg
     3841 gtcggctcca tccaagtccg cggctctggc caggtgcgct acgcgcagct gccgcccggc
     3901 acgttgcggg tgaagggcga ggcggtttgc gcctcgctgc gcggtctgcc ggtcgagccg
     3961 tgcttcaatc tggaacgcac cagcgacaac agcttccgcg gttcgatctc ggggctgggc
     4021 ttcgcctctt gcgaattcaa tcgccacaac gcacgcacaa ccgtgatgca tagcgcgcgc
     4081 agcactcagc cgctcggctt gcgcccttcg ctcaccgcga acaacaacta gagcaatcgg
     4141 cgcttatggt ggcgcgtacc ctgcgtgttg gaagtggttg gcacattcct gcggggtgaa
     4201 ggcctgtagc gcctcgccga cttttcccaa agtgcattgc gggttctcgc tgcagccttt
     4261 ctcagaagcg tcttgggctt ggcgaaggcc atctcgaaag ggttcagatc cggggaatag
     4321 ggtcgaaggt agatgaggcc gtcgggcaga tggggctgaa tcgtgggctg cgtaccagcg
     4381 atggtctgct tgcccggctc tggctggttt ggcttggcgc cgcgctcgtg agcttcggcg
     4441 ctgggccggt gttacggctc tcacgatgtg ccggactgga gcaaagtttc tctcgccgct
     4501 cagattggcg cgaacattgc ttaccctgtc gcctgcgccg ccggatgcct atttctacta
     4561 gccacctgcc tgcggttcag ccgcaatcat ataaaaattc tcgacaacct ctcggcaaac
     4621 gcctatgcga tgtacttggt gcattacatt ttcgtggtgt gtctgcaata cgcccttctc
     4681 ggcagcaacc tgtttccgat cgccaaggtc gccatcgtgc tcagctgcac ccagatcctg
     4741 aattgggcga gcagcactgc tttcaatcgc ttcgcccttg ggccccggcg ctgtgccagc
     4801 caaggagcga ttgggccggt gccgcattga ccggcttttg ggaagagtgg ggaacttcat
     4861 gccgctgaat ctgactctcg cctcgcgtaa tctctttcac gaccggcttc gtttcgtcgc
     4921 caccatggtt ggaatcgttt tttccgtcgt gctcgtgatg gtgcagatgg gtctctatct
     4981 cggctttggg cggatggtga ccacggtgat tgatcacacg tccgccgatc tgtgggtcat
     5041 gcgccacgga actaaatgtt tcgaggaccc atcgctgctg agcgccagca agcagacccg
     5101 tgtcgctgcg accgccggtg ttgccgaggc cattccgctg gtcattggct tttccggctg
     5161 gatcctgccg agcggagaga taacgccggt atttgtcgtc ggctcggact tgcagacctt
     5221 gaaaccatgg aacgtcaccg aaggcacggt tgaggcgctc tcggcacccg gcgcggttgc
     5281 cgttgaccgc tcctatttcg aacggctcgg cgtgtcggga ataggctcaa aggccgatat
     5341 tcgcggccat cccgtcagcg tggctgcggt gaccgacggc atccgctcat ttacgacgac
     5401 tccttacatc ttcagcgaga tgagccgtgc gcgcacctat accggcattc catcgaccat
     5461 ggcgagctac atgctggtca agctaagccc aggcgcggac ctcgagcatg tccgccgcga
     5521 tctccaggcg agcctgcccg actccgatgt tctcacgtca gcagagtttc gcgagcgcag
     5581 ccgctcatac tggctttttg ggaccggggc gggtgccgcg cttttcgcag gcgcaatcct
     5641 ggggataatc gtcggcacag tgattgtcgg gcagacgctc tattcgagca ccaaggacca
     5701 tctcgatgag tttgccacgc tacgcgcgat gggttcctcg cacggctata tctacagcgt
     5761 aatcatctac caggcgctac tcaacgcagt catcggcttc ggccttgcga ccctcatcgg
     5821 atttgccgtc gtgcgattta cgatgagcag cgcgcttccg gtcttgctca cgcccccgct
     5881 tattctcgcg atgctggcct tgaccgtgat tatgtgcgtc gcttcagcgc ttgccgccat
     5941 ctttcgggtg cttcgcgtcg accctgtcat ggtcttcacg cgatgagcgt ttctgttctc
     6001 gaagcaaagg acgtcacaac agtcctgggc cgcggcgctg cgcaggtgca cgctctgagg
     6061 gacgtcaacc tgtcgctgcg gggtggcgag ctgacagtgc tgatggggcc gtcagggagc
     6121 ggcaaaacca cgcttctctc ggttctcgga tgcatgctgg cgcctacctc ggggacggta
     6181 cgagtctgcg gccaaacggc agatgggact gacgccgagg cgctagcgaa actccgccga
     6241 gatcatgtcg gcttcgtttt ccaatcgtat catctgtttc cgacgctcac ggcggcggag
     6301 aatgttcaag tggcgctcga tgtgcgcggc atacatggtg aagaggcgca ggcgaagtcg
     6361 cgcgccgcat tggagcgagt ggggcttgcg cacaagaccg cgagctatcc gctcgcgttg
     6421 agcggtggcg agcagcaacg gatcgcgatc gcgcgcgcga tagtttccga tccttccgcg
     6481 atccttgccg atgaacccac ggcggccttg gatgccggta acggtcaggc catcatgaga
     6541 atccttgccg agatcgccaa ggacccggcg cgcggcgttc tggtcgtgac gcacgattcg
     6601 cgcgtgatgt cgttcgcgga ccgcattata cacatcgagg acggccgtct cgtcggcgac
     6661 gagctcggca gcgctgatgc cgcaaagatg cgaaccgagc ctctctctgg acgtcgtgcg
     6721 tgaacccaag ccacaggatt tcacggcggc catgctgaag aattagctac ccgtatattc
     6781 tggcctacca tatcttctgt gcaatgagcc gaaggccacg ggtcttcggc ctttccgcgc
     6841 ctgcgaccgg ggcgtaagat gagcggcgtt taaggttcca ttgaaaccca ctgcggccac
     6901 tcggttgttg tcgcactgat gtcgcatctt acgataggac ggtcatgtac gctcgcaccg
     6961 gcgcggctct tctgttcatt cttatgaccg gcgctgcggc gctggcggcc aacgaaaggt
     7021 ctcccaattc gtccacaacc cagtcacaag ggacggaaga cgaacaatca gcgtgcacgt
     7081 cggacgcgat gaagttctgc gatgaggata taccgaacac gttcgctgtg ctcgcgtgcc
     7141 ttcaagctca ccgcaaaaga cttaccaagg cctgccgtca cgttttggac tcgaatggtc
     7201 agtagtgcga ttgctggcca gaaagagttt ttgtgccagc gcgatggaag aattattgca
     7261 tttgtggggc ctgatctagc ggtatcagga acgggttcat cgtctgtggc gcaggtggcc
     7321 gatatccgag cgataaatgc tgtctgaccg tgttgcacaa ctcgacaaca taacgccgac
     7381 cgcgccaaag aacgggaagt tttttattcc tggcatccat ggttcggctt tcgggtcttc
     7441 gtgcacgaag tggtggtcag ggaaagtacg cgggtttttc gctttgctgg gacagcgcag
     7501 ttagaggccc actgccttga agtttccgaa tggatgtttg accgagcagc gtgctgcggc
     7561 gtagcgcgcg acgaatcgcg cggttgaact gcactgctct gcagcggttg aagtcattga
     7621 ttcttgaggg ttccggcacg ccgaccggcg cgatgataga ggctcgacct tgttccttgt
     7681 cgctggaagg agaggccgat gcgacgcctg gaccaccgtc gtcatttccc gcaactcgat
     7741 ctgtttcgac cagccgcctc gacactggag tggcaccaac tgcccgtggc ggtgtgtggg
     7801 aaagcgacgc gcctgatggt gagcaggcac acctccccca gccgccgccg cccacgccca
     7861 cgcggtgaaa tgcgcggcgt cggccatggg gatctcctat gcactacatg aataggcccg
     7921 tttcaactca agttgagggg ggtcgcgatt gaacgcgaaa aggggtcggt gttggatgcg
     7981 atttgacatc agcagggacg gatcctcgaa gcacttggtt ccgcgcggct taatccagag
     8041 atcggtggac gcatgatcaa tcaccgtcgt cactttgcgc ccaaaggcaa gatacaagtc
     8101 aatctggacg attacgagaa cgatagaaaa cacgacactg aacacggtcg caatgaagcg
     8161 acgccgatca tgaaacaaat ttcggaatgc gagaatatag attaatggca tgctaacaaa
     8221 actcagtgtc gtatccgtcc aacgaaattt cggccttcac gcaccgcgta ctccccgttt
     8281 tgccgtaacc agtttagaga caaatggtac ggtgcccaac ccggctgtga tggcccagct
     8341 catgatcaag gtgccgccga atacgatcgc ggctttcccg atcgcaagca gcccggtatc
     8401 cagcagcgca tattgcagcc agatggcgaa cacgtaatgg accagatata tcccgtaggc
     8461 attggccgat aggct
//
LOCUS       DASRKK010000562        15583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_243328, whole genome shotgun sequence.
ACCESSION   DASRKK010000562 DASRKK010000000
VERSION     DASRKK010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243328"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /gene="der"
                     /locus_tag="VFC15_12995"
     CDS             <1..1055
                     /gene="der"
                     /locus_tag="VFC15_12995"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZM11116.1"
                     /translation="PNIEPYAQEFRRLGIKELLPISAETGNGVAELLEHVFRVFPRTE
                     RVAEEEAAEVVEEEQAETADGVPVEAEEEEAPKLSKVAIIGRPNVGKSTLLNALTGTG
                     RAIVSPIAGTTRDAVDELVEHDGQTYRFIDTAGIRRKGKTELMAEKLSVVMARKHLEA
                     ADVALILIDAVEGVTASDATIAGYAHESGRSVIIVVNKWDLVTTNRTDGKPPADQKIY
                     EERARRHLKFLNYAPIVFVSASTGKGLNKLYTTLELVAKERRKRIGTGAMNRFLAKVD
                     FERASVPMAKRVRIFYMTQAAVSPPTFILFTDRVVKLHFAYQRFLENQIREAFSFVGS
                     PIWIKNRARHEDRDKK"
     gene            complement(1074..2438)
                     /gene="trmFO"
                     /locus_tag="VFC15_13000"
     CDS             complement(1074..2438)
                     /gene="trmFO"
                     /locus_tag="VFC15_13000"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZM11117.1"
                     /translation="MSPKIRIIGAGLAGSEAAWQCARRGLQVELYEMRPVCSTPAHQT
                     AHFAELVCSNSLKSDAVNTAPWLLKEEMRRAGSLLMEIAAQTAVPAGHALAVDRDRFA
                     EAVTAAIEGEPLIRVVREEVTRIQEEDGITIVASGPLTSDALSQEILRLSGSDQLYFY
                     DSISPIVEADSIDMSRVYMAARYDKGTADYINCPMTGEEYNRFIDALLEAQSVDAKEW
                     EKLNYFEGCLPIEEIARRGRDTLRFGPMKPVGLRDPRTGYTAYAVVQLRQENLRADSY
                     NLVGFQNHLKFGEQARILRLIPGLENAKFLRYGQIHRNTYINGPTLLRETLQMKHHPN
                     VFFAGQISGVEGYVESIATGLLAGMQAAALAIGAEPIAPPRATAFGALVNYICHADAK
                     SFQPANITFDLLLALDEDTRPRIRDKKQRHAMVCERALAELQGWMEAQNPVVTAVVTP
                     PSST"
     gene            2628..3092
                     /locus_tag="VFC15_13005"
     CDS             2628..3092
                     /locus_tag="VFC15_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11118.1"
                     /translation="MRTRTILMTVAFCLIAVALSVAQNPNMGTWQLNEAKSKLAPGFT
                     KNTTVVYAAAGDSIKVTTDGVDKDGKPIHTEWTGKLDGKPYPLTGDPTADARAYTKVN
                     EHTLDLINSKGGKPTTTGKIVVSADGKTRTLTASGTDAAGKKVTSVAVYDKQ"
     gene            complement(3811..4332)
                     /locus_tag="VFC15_13010"
     CDS             complement(3811..4332)
                     /locus_tag="VFC15_13010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM11119.1"
                     /translation="MADDPKYMVKPLGQEDRAAFHCGDPELDSYFHQRASRDMREKLS
                     AVFVLVWKAQPDVVLGYYTLSAQQVDAGELPEDLKKRTGKYKRIGVTLMGRLAVAKEH
                     QGQQLGALLLVDGLRRSLQGTRNVMSFAVVVDAKSDIVAKFYERFGFVRLSGNRLMLP
                     MKTVERNFQPKDN"
     gene            complement(4333..4644)
                     /locus_tag="VFC15_13015"
     CDS             complement(4333..4644)
                     /locus_tag="VFC15_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HZM11120.1"
                     /translation="MATTASKPVKNRSAQTATGRIEARVLPEQKTLFERAAALRGVNL
                     KTFMVDSMQDAALKTLKDHDLIRLTAEERRIFVETLLSPPGRNQALRSATKRYNRLVA
                     G"
     gene            5163..6101
                     /locus_tag="VFC15_13020"
     CDS             5163..6101
                     /locus_tag="VFC15_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11121.1"
                     /translation="MPFDIAEIAGRSIPPLPGKNELWVRKNTRAAAVQLIGAIRTGNE
                     RPVWCDHWRRGRIDFPEGLPAVRHGIVLVETFVPVDLAIEARELPLTYRGVPITYLSV
                     SDFVNLALELRTVPELIAYLDARRAIPLPSLHFVGDEKCLFEFYLLENGTLSGCVGHA
                     DARVVVAARQQELRRLLQEKAVHDRHNKVMEYVADCLAIRNPDYASGLNPDIVAAAFD
                     PSMRRANYIAMQEVVADLRLRERAELGRGLLKAVEHLADKAKGFSFLAAHFTSRDWVW
                     ILASSKDISRSELVGRMMTPLLHAAMAHYITTSPMG"
     gene            complement(6293..8197)
                     /gene="selB"
                     /locus_tag="VFC15_13025"
     CDS             complement(6293..8197)
                     /gene="selB"
                     /locus_tag="VFC15_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZM11122.1"
                     /translation="MKAVIVGTAGHIDHGKSALVRALTGTDPDRLEEEKRRGITIDIG
                     FANLDLKAASGENLRLGFVDVPGHERFVRNMLAGIGGIDLVLLVIAADESVQPQTREH
                     FDICRLLSVQRGITVLTKSDLVDAETLEVVRMEVAEYLQESFLDPASSPIVAVSSKTG
                     AGLDELKRELARLATEAPVKNANAALRLPIDRVFTMKGFGTVITGTLISGSVRKDDEL
                     EVHPGGTKLRVRGVQVHGAAAEKAIAGQRTALNLAGVEVSELARGMMLTAAGLFRPTS
                     QVSVELNLLPSAKPLRDRARVHLHAFTAETIAEVSLLEGKQLKPGQSGLARLKLDAPL
                     LLLPGDRFIVRQFSPVITIGGGQVLDASEPARRAKAAERLAFVKQVAGADAADALLAR
                     IARRGLAGLSVSEAVAETGWLPARVHELSGQLQKAGKVVLCDGLLLTSERFAASRQQV
                     LAAVERFHKENPLVAGINQEQLREQLGVGPEVFRSLLDMLVRDKKLEIAGDQIRGAGR
                     GVVLRDDEAESKQTIEQAFAKAGLQVPALKEVLASLRIDQARAQKIVTLLLRDRVLVK
                     LSDDLVFHRDALEGLRQLVVAQKTKTPKLNVASFKDLASVSRKYAIPLLEYLDRERVT
                     KRVGDERVIC"
     gene            8407..9492
                     /locus_tag="VFC15_13030"
     CDS             8407..9492
                     /locus_tag="VFC15_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZM11123.1"
                     /translation="MSSIVSPPKTPAGTPSQDGRQVAPWDAANPGGDILVPHILLRLE
                     DDLERAHMREAFWLSVAVHVLAVILVVMSPKIFGFKPIQLASPEDIMRNKQLTYLDMP
                     PDMQTPPPKVKPDAKLSDKNRIAESRHPQIDQKTLEEMRKAGPPKAPGQQAPPTEQAP
                     SQQGIQAPQPQQQAQNNPPVQDPKLALPQQNDQKPAVDFRSLNANLGQPATQTARAMG
                     PGRSAGSFGGGGGGGDYGSGRGGSARTAGNLEVLSDTQGVDFGPYLSRVLQAVRMNWY
                     NIIPEAARPPLLERGKVSIEFAILPDGKVAGMHIISPSGDVSLDRAAWGGITASNPFA
                     PLPGEFHGPYLALRFHFYYNPAKGDMQ"
     gene            9514..9687
                     /locus_tag="VFC15_13035"
     CDS             9514..9687
                     /locus_tag="VFC15_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11124.1"
                     /translation="MANEPPAAREQQTRTPEWYERQKSRERMKLTGLLILVLVILLIA
                     FVRFGKTIPWGAG"
     gene            9688..10662
                     /gene="miaA"
                     /locus_tag="VFC15_13040"
     CDS             9688..10662
                     /gene="miaA"
                     /locus_tag="VFC15_13040"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZM11125.1"
                     /translation="MSTLPSTPYDPLLVVILGPTASGKSALAIHLAEKFAGEIVSCDS
                     VAVYRYFEIGTAKPSREDRARVAHHLIDVAEPGDAFSAGEYARQARAALSAIVQRGHL
                     PIVVGGTGLYLRALLEGLFPGPQRSDELRERLRARAAERAPGYLHRVLRRLDPAGALK
                     IHPNDIPKLIRAIEVCLTARGPMSELWQQRGRDALGGFRVLRIGLNPSREKLYERINQ
                     RAAQMFEDGLVAETRSLLARFGDARAITAIHSLGYEQALQHLRGELSLEQAVAAAQQG
                     HRNYAKRQMTWFRREPDVTWIEGFGDDPATTAKAAALVSCELDSAPAL"
     gene            10698..12602
                     /locus_tag="VFC15_13045"
     CDS             10698..12602
                     /locus_tag="VFC15_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZM11126.1"
                     /translation="MFRRPLVLLAVCFVVCVPAFSATRPSFQKPAAVHLDKAGEKWAQ
                     KSLKKMTLEQKIGQMFMIWSHAQFLNVNGVEYVTLRDTMRKYHLGGFGVTVEYEDGFL
                     YKNEPLEASMVTNQLQQDSEFPLIFAADFERGLGMRLNEVTGFPHAMAFGATHNLDYA
                     RQSGRITAMEARAIGVHWNWFPDTDVNSNPDNPVINTRSFGEDPTLVGQMAAAYIEGA
                     HEYGMLTTAKHFPGHGDTDTDSHLAVPLVNGDRTHLDTIELPPFQAAIKAGVDAVLVA
                     HVAVPALDSDPKHVASISPPIVTGLLKQKMGFNGLIVTDALVMDGLMKLFPEGGAAAS
                     GRAAVEAVKAGNDVLLLPADLDAAYNGVLNAVRSGEISESRIDESVLKILRAKASVGL
                     NQARLVDINAVTQVVAKPDSLLVAQRVADSAVTLVRDNKQVLPLTRTPRGTNLPAAAY
                     HPAEDTRGRVLVLIFTEDSRSDAGRLLDEQMRRRIPDARVIYVDPRTAAGLTQPVIDA
                     VDRAHKIIVAIYEVPVAGKVVPGTPGDGNSVAVQSSSAGLLQKVLQTAAGKTVVVAMG
                     SPYVVSQFPEVQTYFCTFSNMHVSEISAVKAMFGEISMSGHMPVTIPNIAVRGAGLNS
                     PALRFSGGSE"
     gene            12626..13183
                     /locus_tag="VFC15_13050"
     CDS             12626..13183
                     /locus_tag="VFC15_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11127.1"
                     /translation="MALVSAALAMSIAACSIHVDDKDKNAKKVDIDTPLANLKVDTSE
                     ASTDNGIPVYPGATPRPESNGDKHRANVNIAAMGFGLKVIAAEYDTPDSPEKVKAFYL
                     DKLSKYGKVLECRGTGGDQGSNAKWNDNEELSKPVTCDDTKGDGWELKTGTNGNQHLV
                     AIHPEGSGARFGTVLVQIHGKESTL"
     gene            13248..13985
                     /locus_tag="VFC15_13055"
     CDS             13248..13985
                     /locus_tag="VFC15_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11128.1"
                     /translation="MRQSVAAVTFLVVEHLCYKCATSIDETLPFCPHCGAPQIHVASP
                     EEDASQAPAPFSDDAGPRLVTPSTWPSGASANIQQTSAIQWELAWKGALLCGLGAAVL
                     TAMPVLGLGCCLWLLGAGALAVWLYQRRVPGSVVTPGMGMRIGAVSGAIGYVATTIWS
                     VFRFASNSQEFRTALQEQMDKSLAANPDPRAQEIMRQFMGNLNTPQGLATFFVLVMAI
                     MAVVFVIFSAAGGALGASMFARRRDFR"
     gene            complement(14001..14972)
                     /locus_tag="VFC15_13060"
     CDS             complement(14001..14972)
                     /locus_tag="VFC15_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11129.1"
                     /translation="MAKSDPIEHSLDRLGELRRAAASETSMDEVRQFLRNRSNLVVAK
                     AAIVVRELQIKALIPDMVSAFNKLMADAPRLDKRCAATTEIVSGLYELSYEYPAPYLM
                     GLKHVQMEASFGPPVDEAAKLRAVSAQGLVRTRHPDALTEVMQLLVDREPAARIGAVR
                     ALAVNGGEAGILLLRLKVLTGDAESAVLGECFSGLLEASPVKSVPFVAEYVDSKDGDV
                     AQATMLALGESRLPAAYEVLRQKWSRTAGIPAKKILLASMAASKLDEAIAFLISLVET
                     ESIPTAAAVVEALSVYSRNERVRKSMNDAVVARRDQTLADHFKREFG"
     gene            15282..>15583
                     /locus_tag="VFC15_13065"
     CDS             15282..>15583
                     /locus_tag="VFC15_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZM11130.1"
                     /translation="MSASEIDRTIVRLAHEIVEKNNGADNLGLVGIMRRGVPLAQRLA
                     KILGRIEKSEVPVGTLDITLYRDDLSTIGHRPEVRKSKMEFDIQDKNIILVDDVLYT"
BASE COUNT         3151 a         4760 c         4496 g         3176 t
ORIGIN      
        1 cgcccaacat cgagccctac gcgcaggaat ttcgccgcct cgggatcaag gagttgctgc
       61 ccatctcagc cgagaccggg aacggcgtgg cggaattgct ggaacatgtg tttcgcgtct
      121 ttccccgtac cgagcgcgtc gccgaagaag aagcggcaga agtcgtcgaa gaagagcaag
      181 ccgagaccgc tgacggcgtg ccggtcgaag ccgaagaaga agaggctccg aagctttcca
      241 aagtcgcgat catcgggcgc cccaacgtcg gcaaatccac gttgttgaac gcgctcaccg
      301 gcaccggtcg tgccatcgtt tcacccatcg cgggaaccac gcgcgatgcc gtcgatgaac
      361 tcgtcgagca cgacgggcag acctatcgtt tcatcgacac cgccggcatt cgccgcaagg
      421 gcaagaccga gttgatggcc gaaaagcttt ccgtcgtgat ggcgcgcaag catcttgaag
      481 ccgctgatgt cgcgctgatc ctgatcgatg cggtggaagg cgtgaccgcc agcgatgcca
      541 ccatcgccgg ctacgcgcac gagagcggac gctccgtcat tatcgtggtc aacaagtggg
      601 acctggtcac caccaatcgc accgacggca agcctcccgc cgatcagaag atttacgaag
      661 agcgggcgcg gcggcatctt aagttcctaa actatgcacc tatcgtgttt gtctcggcct
      721 cgaccggcaa gggactcaac aagctgtaca cgacgctgga actggtcgcg aaagaacgtc
      781 gcaagcgcat tggcaccgga gctatgaacc gcttcctcgc caaggtcgac ttcgagcgcg
      841 cctcggtccc aatggcgaag cgagtgcgca tcttctacat gacccaggca gcagtctcac
      901 cgcccacttt catcctgttc accgaccgcg tggtgaaact gcacttcgcc tatcagcgtt
      961 ttctggagaa ccagattcgc gaggcgttca gctttgtggg ttcacctatc tggatcaaga
     1021 accgcgcccg ccacgaagat cgggacaaga aataactgct cgtaagacgg tgactaggtc
     1081 gacgagggtg gcgttacaac ggcggtcaca accggatttt gcgcttccat ccatccctgc
     1141 aactccgcca atgcccgctc gcacaccatg gcatggcgct gcttcttatc gcgaatccgg
     1201 gggcgggtgt cttcgtccaa cgccagaagc aagtcgaacg taatattcgc cggctgaaaa
     1261 ctcttcgcgt cggcatggca gatgtaattc accagcgccc cgaaggccgt cgcgcgcggc
     1321 ggtgcaatcg gttctgctcc gatcgccagc gccgctgcct gcatgcccgc gagcagtccg
     1381 gtggcgatgg actcgacgta cccttcgacg cccgaaatct gtccggcgaa aaacacgttg
     1441 ggatggtgct tcatctgcag cgtctcgcgc aacagtgtcg ggccgttgat gtaagtgttg
     1501 cggtgaatct gtccgtagcg caggaacttc gcattctcga gcccggggat gagtcgcaga
     1561 atcctcgcct gctcgccaaa cttcagatgg ttctggaagc cgaccaagtt gtaggaatcg
     1621 gcgcgcaggt tttcctgccg taattgcacc accgcatacg cagtgtatcc ggtgcgcgga
     1681 tcgcgcagcc caactggctt catcgggccg aagcgcaggg tgtcgcgtcc acggcgcgcg
     1741 atttcttcga tggggaggca accctcgaag tagttcagct tctcccactc tttcgcatcg
     1801 accgattgtg cctcgagcag cgcgtcgata aaccggttgt actcctcccc ggtcatcggg
     1861 caattgatgt agtcggcggt gcccttgtcg tagcgagcgg ccatgtacac ccgcgacatg
     1921 tcgatggaat cagcctcaac aatggggctg atcgagtcgt agaagtaaag ctgatcgctg
     1981 ccgctcagcc gcagaatctc ctgtgatagc gcgtcggaag tgagcggccc gctggcgacg
     2041 atggtgatgc catcttcctc ctgaattcgc gtcacttctt ctctcactac gcgaatgagc
     2101 ggctcgcctt cgatggctgc ggtcacagct tctgcgaacc ggtcgcgatc gacggccagg
     2161 gcgtgtcccg cgggaactgc tgtctgcgcg gcgatctcca tcagcagcga acccgcgcgc
     2221 cgcatctctt ctttcagtag ccacggagca gtgttcaccg cgtcggactt cagcgaattt
     2281 gagcacacca gctcggcgaa atgtgcagtc tggtgcgcgg gagtcgagca gaccggacgc
     2341 atctcgtaca gctcgacctg caggcctcga cgggcgcatt gccaggccgc ctccgagccg
     2401 gcaaggccag cgccgataat gcgaatcttg ggtgacatga atcctgcaga ctgtcggtat
     2461 tatacggacg agtcactcgg cggacctcga gttcccggag gaacagattt cggagtagtt
     2521 gcgctgcgga accctgggtc agggtatttc cagatccccc acatacgtgt atagtttgtg
     2581 gctgcgtcag aactgcacct caaatcccca tccaaacagg agaacacatg agaacacgca
     2641 ccatcttgat gactgtcgcg ttttgcctca tcgcagtagc gctatctgtc gcccagaacc
     2701 cgaacatggg cacctggcag ctgaacgaag ccaagtcgaa gctcgctccc gggtttacca
     2761 agaacaccac ggttgtctac gcggccgcgg gtgacagcat caaagtcacc accgatggcg
     2821 tcgacaaaga cggcaagccg atccacaccg agtggacggg caagctcgac ggcaaaccct
     2881 acccgctgac cggcgatccg acggccgatg cgcgcgccta caccaaggtc aacgagcaca
     2941 ccctggacct gatcaactcc aagggcggca agcctactac caccgggaag atcgtggtct
     3001 ccgccgacgg taagacccgt accctgaccg ccagtggaac cgatgcggcc gggaagaaag
     3061 tcaccagcgt tgcggtgtac gacaagcagt aagaacctca ctgttctctt tgagtcgccc
     3121 actcaagctt cggcttgggt gggccttgtt ttttggaccg tccgtaggaa aactgccaaa
     3181 attgcttttc acgtgttgtt tgttttcatg aggttagact gaaattgctc gcatgtcatt
     3241 gattccaagt ttgttatagc tatccatgta agccgtaagg agttatctct gcaatttcct
     3301 gcaaccttcg cgattttccc gcaatactgt ctcgccgcat tactggttca tgaacttttc
     3361 tgacaactac caaaattcct tggaatcatc gagttggcgc gaattttcac gcaaaataat
     3421 tagtttgtta gacttacggg taaaatattc taatcaaata acttagggca ctcgatcgac
     3481 gggactgacc cgggtcgccg atgccgtccg aactcgtcga tttcggcggg tcgggcaggc
     3541 gccgagttcc gcgccggtca ttcggagcgg aacatgccag cttcagggat aaggcttcga
     3601 agagggccga gtcacgcgaa atcgaacgtc cgaggttacg aaggtaacga gaattcccgg
     3661 aaaggaatcg agttggcatc catccgaaag ctggtgattc atcactcgca gcgtgtcgtc
     3721 gaaaagggga acgacaaggg tgggacagcc gcccagtcag gtcaggtccg aaagatctag
     3781 ctggcagggg tgggtcgccc gccgccaaaa tcaattgtct ttaggctgga agttccgttc
     3841 cacagttttc atggggagca tgaggcggtt gccgctcaag cgcacaaatc cgaacctctc
     3901 atagaatttg gcgacgatgt cgcttttcgc gtccaccacg acggcaaacg acatcacgtt
     3961 tcgcgttccc tgcaggctgc ggcgcaaccc gtcaaccagc aacaatgcgc ccaactgctg
     4021 tccttgatgt tctttcgcta ccgcaagacg tcccatgagt gtcacgccaa ttcgcttgta
     4081 ctttccggtc cgtttcttca ggtcctcggg aagctcaccc gcatcgactt gctgcgccga
     4141 taacgtgtag tacccaagga ccacatccgg ctgggctttc caaacgagaa cgaagaccgc
     4201 cgaaagtttt tccctcatat cgcgcgatgc ccgttgatga aaatagctgt cgagttccgg
     4261 gtccccacag tgaaaggccg ctcggtcttc ctgaccaagc ggcttcacca tgtatttggg
     4321 atcgtctgcc aattatccag cgaccaatcg gttgtaacgc ttggttgccg accgcagcgc
     4381 ctggttacgc ccaggggggc tcaacagcgt ttcaacgaag atccgacgct cctcagccgt
     4441 gagccggatc aagtcgtggt ccttgagggt cttcaaggcc gcgtcctgca tggagtcaac
     4501 catgaaggtc ttcaagttga cgcctcgaag cgccgccgcg cgctcgaaca gcgtcttctg
     4561 ctcgggaagc acacgggcct cgatccgtcc cgtcgcagtc tgggccgacc ggttcttgac
     4621 cggcttgctc gccgtggttg ccatatggcc ctccgtttca aatccacatc aagtgtacgg
     4681 ctgccagacg tacaagtcaa acaatctgta cggcaaggtg ccgtactaac agttccgcgc
     4741 caagtatagc tctgatgcgg cgtgccacac tcgccccgca ggcatgcggt aactggccgc
     4801 taccgtctat cagcatgctg gtgatgcggt gggaacccgc acagcccaat cccttcaaaa
     4861 cctttttctc tccgcacagc gtgtacagcg ccgcaagaac aatcgagccg cacagccggg
     4921 gtccatcatg gacgccgtta taccgtccac cagcttgttc agcgagtgca gggacgcatg
     4981 ccaatttcac gagtaaacta gggattaatg gcgatcacca acacccgcac gcgatggtct
     5041 gaacggcata tggaagaact ggtgtcgttg ccgtttattg cggagtttgt ttttcggagc
     5101 ccaaagaagc tcgacccaca ggagaaggaa gttgcggatc tgatggttgt ccacgagggt
     5161 ggttgccttt tgatatcgca gaaattgcag gaagatccat tccgccgctc cccggaaaaa
     5221 atgagctgtg ggtacgcaag aacacaaggg ccgctgctgt tcagttaata ggggcgatac
     5281 gtacagggaa tgaaagaccc gtttggtgcg accattggcg tcgcggaagg attgattttc
     5341 ctgaagggct gccagcggtt cgccacggca tcgtgttggt ggaaacattt gtgcccgttg
     5401 acttggcaat agaggctagg gagttaccac tgacgtatcg tggggtaccg atcacctact
     5461 tgtcagtgag tgatttcgtt aatctggcac tggaacttag gaccgtcccg gagcttatcg
     5521 cctaccttga tgcgcgtcgc gccataccac tcccctcact acatttcgtg ggagacgaaa
     5581 aatgtttgtt cgagttttac ctgctcgaga atggcactct atcgggttgc gtcggacatg
     5641 cggacgcaag ggtagttgtg gccgcgcgtc aacaagaact gcgccgatta ctccaagaaa
     5701 aggctgtgca cgatcgacat aataaggtga tggaatacgt cgccgattgt ttggccatac
     5761 gaaaccctga ttatgcttct ggcttgaatc cagatatcgt cgccgccgcg tttgacccat
     5821 cgatgcgacg agctaactac atcgcgatgc aggaagtggt agcagacctt cgcctaaggg
     5881 aacgtgccga actaggtcgc ggactgctca aggcggtcga gcatcttgcg gacaaagcga
     5941 aagggttttc tttcctggcg gcgcatttca cttcccgcga ttgggtttgg atactagcct
     6001 cgtcgaaaga catcagccga tccgagttgg tcggccgcat gatgacgcca ctgctgcacg
     6061 cagccatggc gcattacatt acaacaagtc caatgggatg attatcgttc gccgagatgg
     6121 tgggggcttt gaggttggct tgtcacgacc gggatttaag cccgacccat cggattacgc
     6181 ggccggcgaa caagcattcg gtcggctgcg tgtgacttca acaccgctga aagtggtgta
     6241 gcacgcggct taaaccggta ggtggacgac ttttcgtatt gcagaatggc gattagcaaa
     6301 tcaccctctc atcccccacc ctctttgtta cccgctcgcg gtcgaggtat tccagcagcg
     6361 gaattgcgta cttgcgcgag acgctcgcta ggtccttgaa cgaggcgacg ttcagttttg
     6421 gggtcttggt cttctgcgcc actaccaact ggcgcagacc ttcgagcgcg tcgcgatgga
     6481 agacgagatc gtcggacagc ttgaccagca cgcgatcgcg gagtagcagc gtgacgatct
     6541 tttgcgcgcg ggcttggtcg atacgcagcg acgccagcac ttccttgagc gccggaacct
     6601 gcagtccggc tttggcgaag gcctgttcga tggtctgttt cgattcggct tcgtcgtcgc
     6661 gcagcactac gccgcgtcct gcgccgcgaa tctgatcgcc tgcgatctcc agctttttgt
     6721 cgcgcaccag catgtcgagc aaactgcgaa agacctccgg gccaacgcct agctgctcgc
     6781 gcaactgctc ctggttgatg cctgcgacca gcggattctc tttatgaaac cgctccaccg
     6841 ccgcgagaac ctgctgccgc gatgcggcga accgttctga ggtgagcagc aggccgtcgc
     6901 agagcacgac tttcccggcc ttttgtagtt ggcccgagag ttcatgcacg cgtgcgggaa
     6961 gccatccggt ttcggcgacg gcctcggaca ccgagagtcc ggccaagcca cgtcgcgcaa
     7021 tccgcgcgag caaagcgtct gcggcatctg cccccgccac ttgcttcaca aacgccagtc
     7081 gctctgccgc cttcgcccgc cgcgcaggct ctgacgcatc gagtacctgc ccgccaccga
     7141 tggtgatcac cggcgagaac tggcggacga tgaaacgatc gccgggcagc agcagcaacg
     7201 gagcatcgag cttgaggcgg gccaggcctg actgtccggg cttgagctgc ttgccttcga
     7261 gcagactgac ctctgcaatc gtctcggcgg tgaaggcgtg cagatgcacg cgcgcgcggt
     7321 cacgcagtgg cttggccgac ggcagcagat tcagttcgac actcacctgg ctggttgggc
     7381 gaaagagtcc ggcagctgtc agcatcatgc cgcgggcgag ttcgctgact tcaacacccg
     7441 ctagattgag cgcggtgcgt tgtccggcaa tggctttttc ggcggcggct ccgtgtacct
     7501 ggactccgcg cacccgcagc ttcgtcccac ccggatgtac ttccaactcg tcgtctttgc
     7561 ggacgctgcc ggagatcagc gtgccggtga tgacggtgcc gaatcccttc atggtgaaga
     7621 cgcggtcgat gggcagacgc agcgcggcgt tcgcattctt gaccggagcc tcggtcgcca
     7681 gccgcgctag ttcgcgcttg agttcgtcga gccctgcgcc agtcttggaa ctcacggcca
     7741 cgatggggga gctcgccgga tcgagaaacg attcctgcaa atactcggcc acttccatgc
     7801 ggaccacttc gagcgtctcg gcatcgacaa ggtcggactt ggtcagcacc gtgatgccgc
     7861 gctgcaccga cagcaggcgg cagatgtcga agtgctcgcg ggtttgcggc tgtacggatt
     7921 cgtctgcggc gatcaccagc agcaccaggt cgatgcctcc gatgcccgcc agcatgttgc
     7981 gcacgaagcg ctcgtgtccg gggacatcga cgaagccgag ccgcagattc tcgccagaag
     8041 ccgccttcaa atcgaggttg gcgaagccga tgtcaatggt gatgccgcgc cgtttctctt
     8101 cttccagacg atcgggatcg gtgccggtca gcgcgcgcac caacgcgctc ttaccgtggt
     8161 caatgtggcc cgcggtcccg acgatgacgg ccttcatgcc tccagtgtaa acaagtgtta
     8221 atggaagtcg gggagcgctt aggtggaata acctaatagg cccctgatat gcgagaatag
     8281 agggtagaga gttgttgtat gctgccgggt ttactatggt tgcgccgtct ccgaggagag
     8341 ttgtgttctt ctgggggatc gaggggcgcc gaagtggttg agctcgtgcg tacttgatcc
     8401 atacggatgt catccatcgt tagtccgcca aaaactccag ccggcactcc ttcgcaagac
     8461 gggcgccagg tcgcgccttg ggatgcggct aatccgggcg gtgacatcct tgttccgcac
     8521 atcctgttgc ggctggaaga cgacctcgaa cgcgcgcaca tgcgcgaggc gttctggctc
     8581 tcggtagccg tccacgttct tgccgtaatc ctggtcgtaa tgtctcccaa gatattcggc
     8641 ttcaagccca tccagctcgc gtcgcctgaa gacatcatgc ggaacaagca gttgacgtac
     8701 ctcgacatgc ctcctgacat gcagacgccg cctccgaagg tgaagcctga cgctaagctg
     8761 tcggacaaga accgcattgc cgagagccgg catccccaga tcgaccagaa gacgctggaa
     8821 gagatgcgca aggctggacc tccaaaggcg ccggggcagc aggctccgcc tacggaacag
     8881 gcgccttcgc agcagggaat ccaggcgcca caaccacagc agcaggcgca gaataatcct
     8941 ccggtgcagg accctaagct cgcgttgccg cagcaaaacg accagaagcc ggccgtcgat
     9001 ttccgcagcc tgaacgccaa tcttgggcag ccggcaacac agactgcgcg ggcgatgggg
     9061 cccggccgtt ctgccggatc gtttggtggt ggaggaggcg gcggagacta cggctccgga
     9121 cgtggtggca gcgctcgtac cgccggcaat cttgaagtcc tcagcgatac ccagggcgtc
     9181 gatttcgggc cctatctttc gcgcgtgcta caggccgtgc gcatgaactg gtacaacatc
     9241 attcccgagg cggcgcgtcc gccactgttg gaacgcggca aggtgtcgat cgagtttgcc
     9301 atcctgcccg atggtaaggt cgcggggatg catatcatct ctccgtcggg ggacgtctcg
     9361 ctggatcgcg ctgcgtgggg cggcatcacc gcgtcaaacc cgttcgcgcc gctgcccggc
     9421 gaatttcacg gaccgtactt ggcgttgcgc ttccactttt attacaaccc cgctaaaggg
     9481 gatatgcagt aacgcggcgc gtaaaatggc gctgtggcca acgaaccgcc ggcagcgcgc
     9541 gagcaacaaa ccagaacacc ggaatggtat gagcggcaga agtcgcgcga gcgcatgaag
     9601 ctcaccggcc tgctcattct ggtgctggtg attctgctca tcgcgttcgt gcgcttcggc
     9661 aagaccattc cgtggggcgc ggggtgaatg tcgacacttc cgtcaactcc ttatgatccg
     9721 ctactagtcg tgatcctcgg accgacggcc tcgggcaagt cggcgctcgc catccatctc
     9781 gccgagaaat tcgcgggcga gattgtcagt tgcgactcgg ttgctgtgta tcgctatttc
     9841 gagattggta cggcgaagcc ttcgcgcgaa gatcgcgctc gcgttgcgca tcacctgatc
     9901 gacgtcgccg agcccggaga cgcgttcagt gccggggaat acgcccgcca ggcacgcgcg
     9961 gctttgtcgg cgattgtgca gcgtgggcat cttcccatcg ttgttggcgg cactgggctt
    10021 tacttgcgcg ctttgctgga aggcttgttc cccggcccgc agcgatcgga cgagcttcgc
    10081 gagcgcttgc gggcgcgtgc ggcggagcgc gctccaggat atctccatcg cgtgttgcgc
    10141 cgtctcgatc cagcaggcgc attgaagatc caccccaacg atattcccaa actcattcgg
    10201 gcgatcgaag tctgcctaac cgctcgcggc cccatgagcg aactctggca acagcgcgga
    10261 cgcgatgccc tgggcgggtt cagagtcctg cgcattggac tgaatccgtc gcgcgagaaa
    10321 ctgtatgagc gcattaatca gcgtgccgcg cagatgttcg aggatggctt ggtcgctgaa
    10381 acgcgatccc tgctggcacg attcggcgat gcgcgcgcca tcactgcaat ccactcgctt
    10441 ggatatgagc aggcgctgca gcatctgcgc ggagaactct cgctggagca ggcggttgct
    10501 gccgcacagc agggacatcg caactatgcc aagcgccaga tgacgtggtt ccgccgcgag
    10561 cctgatgtca catggattga gggcttcggc gacgatcccg ccaccacggc caaagcggca
    10621 gcattggttt cgtgcgaact agactcggca cccgcgctct gacaccccat ttcgcttcat
    10681 gctattgtca ctgcgtcatg ttccggcgcc cacttgttct gctggcagtc tgcttcgtcg
    10741 tctgcgtgcc cgcattcagc gccaccaggc cctccttcca gaagcccgcc gccgttcatc
    10801 tcgacaaagc tggagaaaaa tgggcgcaga aatcgctcaa gaagatgacc ctcgagcaga
    10861 agatcgggca gatgttcatg atctggtcgc acgcgcagtt tctcaacgtg aacggcgtgg
    10921 agtacgtcac gctgcgcgac accatgcgca agtatcacct cggcggtttt ggcgtaaccg
    10981 tcgaatacga agacggattc ctgtacaaga atgaaccact cgaagcttcc atggtgacca
    11041 atcaactgca gcaggactct gagttcccgc tcatctttgc cgccgacttc gagcgcggcc
    11101 tcggcatgcg tctcaacgag gtcacgggat tccctcacgc catggcgttt ggcgccaccc
    11161 ataaccttga ctacgcacgc cagtcaggac gcatcactgc catggaagcg cgggctatcg
    11221 gggtgcattg gaactggttt cccgacaccg acgtgaactc caatcccgac aatccggtga
    11281 tcaacacgag atcctttggc gaggacccga ccctcgtcgg ccagatggcc gctgcctaca
    11341 tcgagggcgc gcacgagtac gggatgttga ccaccgcgaa gcattttccc gggcacggcg
    11401 acaccgacac tgactcacat cttgctgtcc cgctggtgaa tggtgatcgc acgcacctcg
    11461 acaccatcga actcccgccg ttccaagccg ccatcaaggc cggagtcgat gccgtgctgg
    11521 tggctcacgt cgcggttccc gcactcgatt ccgatcccaa acacgttgct tccatctcgc
    11581 cgccaatcgt taccggactg ctcaaacaga agatgggctt caacggactc atcgttaccg
    11641 acgcgctggt gatggatggc ctcatgaaac tgtttcccga aggtggcgct gcggcctcgg
    11701 gtcgcgccgc ggtcgaagcc gtgaaggccg gcaatgacgt tctacttctg ccggcagatc
    11761 ttgacgctgc ctacaatggt gtcctgaacg ccgtgcgtag cggcgagata tccgaatcgc
    11821 gcatcgacga atcggtgctc aaaatccttc gcgccaaagc ttcggtggga cttaaccagg
    11881 cgcgactggt ggatatcaac gctgtaaccc aagtcgtcgc caagcccgac agcctgctcg
    11941 tcgcgcaaag agtcgccgac agcgccgtca cccttgtccg cgacaataaa caagtgcttc
    12001 ctctgacccg cacccctcgg ggaaccaacc tgcccgctgc tgcgtatcac cctgcagagg
    12061 acacgcgagg ccgcgtcctg gtgctcatct ttaccgaaga cagccgctcc gatgccggac
    12121 gtctgctgga cgaacaaatg agacggcgga ttcctgacgc ccgcgtaatc tacgtcgacc
    12181 cgcggactgc cgccggcctg acacagcctg tgattgacgc ggtggatcgc gcgcataaga
    12241 tcatcgtggc gatttacgaa gttccggttg cgggtaaagt agtgccgggg actcccggcg
    12301 acggcaacag cgtggcggtg cagagttcgt cagctggact gttgcagaag gttttgcaga
    12361 cagcagccgg caagactgtt gtggtggcga tggggagtcc ttatgtggtc tcccagtttc
    12421 ccgaggttca aacgtacttt tgcacatttt ctaatatgca tgtttccgag atcagcgcgg
    12481 tcaaggcgat gtttggcgag atttccatgt ctggccatat gccggtgacc attcccaaca
    12541 tcgcagtccg cggcgcggga ctcaacagcc cggccttacg tttcagtgga ggatcggaat
    12601 gaaaaccgtt atgcgcagaa tttcaatggc cctcgtgtcc gcggcgctgg cgatgtcgat
    12661 cgcggcgtgc tcgatccacg ttgacgacaa ggacaagaac gccaagaagg ttgatatcga
    12721 tactcccctt gccaatctga aggtcgatac cagcgaagct tcaaccgaca acggaatacc
    12781 cgtttatccc ggggctacgc cacggcccga gtcaaatggc gacaagcacc gcgctaacgt
    12841 caacattgca gcaatgggct ttggcctcaa agtcatcgcc gccgagtacg acacacccga
    12901 ctcgcctgaa aaggtgaagg ccttttatct cgacaagctg tcgaagtatg gcaaggtgct
    12961 cgaatgccgc ggcactggtg gagaccaggg ctcgaacgcc aagtggaacg acaatgagga
    13021 actcagtaag ccggtcacat gcgacgatac caagggcgac ggatgggaac ttaagaccgg
    13081 caccaatggc aaccagcacc tggtcgcgat ccacccggaa ggttccggcg cgcgcttcgg
    13141 taccgtgctg gttcaaatcc acggcaaaga gagcacgctg taacgacacg ctggtctgtg
    13201 ctcgcttgaa actcgaaact tgaaactcga gaccggattg ctccgggttg cgtcagagtg
    13261 tcgctgctgt tacgtttctc gtcgtggaac acctctgcta taagtgcgcg acttccatcg
    13321 acgagacact ccctttctgt ccccactgtg gcgcgcctca gattcatgtt gcctccccgg
    13381 aggaagacgc gtcgcaggcg ccagcgccat tttccgacga tgcgggaccg cggctcgtca
    13441 ccccttctac gtggccttcg ggcgcttcgg ccaacatcca gcagacctcc gcgatccagt
    13501 gggagctggc gtggaaaggc gccctgctct gcggtctcgg cgccgccgtg ctcaccgcga
    13561 tgcccgtgct gggtttggga tgctgtctat ggttgctcgg cgcgggagcc ctcgccgtct
    13621 ggctctatca acgtcgcgtg cccggcagcg tggtcacccc gggcatggga atgcggatcg
    13681 gcgcagtctc cggcgcgatc gggtatgtgg ccaccaccat ctggagcgtg tttcgctttg
    13741 cctccaacag ccaggaattc cgcaccgcct tgcaggaaca aatggacaag tccctggctg
    13801 ccaaccccga tccccgcgcc caggaaatca tgcgccaatt catgggcaac ctgaacaccc
    13861 cgcaggggct ggccaccttt tttgtcctcg tcatggcgat catggcggtg gttttcgtga
    13921 tcttcagcgc cgccggaggg gcgctcggtg cgtctatgtt tgcccgccgc cgcgacttcc
    13981 gctaggacag cggagcatcc ctaaccgaac tcgcgtttga aatggtcggc aagggtttgg
    14041 tccctccgtg ccaccacggc gtcgttcatg gacttcctca cgcgttcatt ccggctgtaa
    14101 acggagagcg cctcaaccac agctgcagct gtaggtatgc tctcggtctc gacaagcgaa
    14161 atgaggaaag caatcgcctc atcgagcttc gatgctgcca tcgacgccaa tagaatcttc
    14221 ttcgccggaa tccctgccgt cctggaccat ttctgtcgca ggacctcata ggcagccgga
    14281 agacgggact cgccaagcgc gagcattgtg gcctgtgcca cgtcaccgtc tttggagtcg
    14341 acgtactctg cgacaaaagg gacggacttg accggggagg cttcgagaag cccggaaaag
    14401 cattcgccta gtaccgcgga ttcagcgtca ccagtcaaca ccttcaacct aagcaggaga
    14461 atgccagctt ctcctccatt tacggccaat gctcgaactg caccaatcct tgcggcaggt
    14521 tcacggtcga ccagtaactg catgacctca gtcagcgcgt cgggatggcg cgttcgaacc
    14581 agcccttggg cgctcactgc ccgcagcttc gctgcttcgt cgaccggcgg cccgaacgac
    14641 gcttccatct gaacatgctt aaggcccatc aagtaaggag ccgggtactc gtagctcagt
    14701 tcgtaaagcc cacttacaat ctcggttgtc gcggcgcagc gcttgtcgag ccgtggcgcg
    14761 tcggccatga gcttgttgaa agccgacacc atgtcgggaa tgagcgcctt gatctgtaat
    14821 tcccggacga cgatcgcggc cttagcgaca accaagttgc tgcgatttcg caggaattgg
    14881 cgaacttcgt ccatcgaagt ttccgacgcg gccgcacggc gcaattcacc cagcctatcc
    14941 agcgagtgtt caatcggatc gctcttcgcc atctcagata tctttcatcc ttcggagatc
    15001 tttcgcgcgg tttagaccga cgatttggtg cctcgtctac actacactcg aaccttatga
    15061 ccttcgtaac ccgcccgact tgctgtctgc ctccgcccgc cgcgccgccg cccttactgt
    15121 cggatcgttc gtaggcatca ccgctcccgt caccctcttc cgtgttctcc atcacacttc
    15181 tgcgtcccca gttgctcctc ccctcaccca tttcgtatca ttcggactgc atgactaaac
    15241 cctccagccg gcctcctact cttcgcgaga aatcgcaaat catgtccgcc tccgaaatcg
    15301 atcgcaccat tgtccgcctc gcccacgaga tcgtggagaa gaacaacggc gccgacaatc
    15361 ttggactggt cggcatcatg cgtcgcggtg ttccgctggc gcagcgcctg gcgaagatac
    15421 tcgggcgcat tgaaaagtcg gaagtgccgg tcggcacgct cgacatcacg ctttatcgcg
    15481 acgacctctc caccatcgga catcgccccg aagtgcgcaa gagcaaaatg gagttcgaca
    15541 ttcaggacaa gaacatcatc ctggtggacg acgtgctcta cac
//
LOCUS       DASRKK010000563         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_928350, whole genome shotgun sequence.
ACCESSION   DASRKK010000563 DASRKK010000000
VERSION     DASRKK010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928350"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFC15_13070"
     CDS             complement(<1..316)
                     /locus_tag="VFC15_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11131.1"
                     /translation="MAVLLIAVGANAQETKPVPPTPIDVKKGDLGGKVWNPAWDLIVE
                     KAIPPAMLSSAVSHDVARFCPRFSTMNDRDKRVFWAYFFQALAGAEAGLNPRARVRHT
                     EPE"
     gene            817..966
                     /locus_tag="VFC15_13075"
     CDS             817..966
                     /locus_tag="VFC15_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11132.1"
                     /translation="MHKNQPRQESNQALLARVKKEMDEPRMAESTREEYDVNHTTLRE
                     LKKLS"
     gene            1029..1250
                     /locus_tag="VFC15_13080"
     CDS             1029..1250
                     /locus_tag="VFC15_13080"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HZM11133.1"
                     /translation="MAKLTKAGRDEISAANFAFPKQRKEPLENASHVRNAVARFSQVK
                     DVTEAERQAAWKRIQAAAKLYGVELQQSK"
     gene            complement(1319..>2321)
                     /locus_tag="VFC15_13085"
     CDS             complement(1319..>2321)
                     /locus_tag="VFC15_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZM11134.1"
                     /translation="NVNSETVYRALVRNKAQLTYNAVGSWLEGTMAAPPKVASSTDLQ
                     EQLKLQNEVAQVLKRQRFLHGALNLDTNEVLPVVLNQQVVDIAKQVRNHATDLIEDFM
                     IGANGVVARLLEKVSSLRRIVKTPERWDRIVQLAAARGETLPVQPDSKALNDFLLKRK
                     SADPDHFADLSLAVIKLIGPGEYVLERPGDPEQGHFGLAVQDYTHSTAPNRRFADVVT
                     QRLVKTFLAGTSGPYTDDELADIAKNCTLKEDAARKVEREMSKRLAAVVMSQRIGETF
                     DAIVTGVTPHGTFVRVMQPHIEGLLAQGHQGLDVGDKLRAKLTRTDVQHGYIDFVCA"
BASE COUNT          542 a          669 c          614 g          496 t
ORIGIN      
        1 cctccggttc ggtatgccgt acgcgggcgc ggggattcag ccccgcctcg gcgcccgcga
       61 gtgcctggaa gaaatacgcc cagaagacac gcttgtcgcg gtcgttcata gtcgaaaagc
      121 gtggacagaa acgcgcgaca tcgtgggata ccgcagacga tagcatcgcc ggcggaattg
      181 ctttctcaac gatgagatcc caggcggggt tccatacctt tccgcctagg tcacctttct
      241 tcacgtcgat gggagtcggg ggcacaggtt tcgtctcttg agcattggcc ccgactgcga
      301 ttagcaaaac tgccatgaca aagacttgca ccctagtggc tcgcacggtg gcatgttgtc
      361 gttcacgctt cctgccttcg cgctcttcac ttgtcatttc cgttgcccgg atttctgtga
      421 tgggatgatt tcgtcgcaac gagcatttcg aggtcgaagg ggaaacaatt ccgaaccgat
      481 gaataacgca ggttgcgtct cctctcaaga atggaatcac acctgctcag gactctggat
      541 cggcgtagat taagatgttg gctccattca gagaaacagt aaaaacctga acttcactgc
      601 ctcataccct ccgccaatct tctgacactg ttcgtcttcg acagtccaag cgtccgatcg
      661 cgtgcacgaa acgctgtatt gcacgttcta cgatgctacg tttcgcagaa cggtacgaac
      721 tgagcaaaac tgctcgacag gaaaaacaat tccacagttg tcagccctgt tcccgattga
      781 cctgtaatgc tcatggactc gtgaaatcct gcaagtatgc ataagaacca gccaagacaa
      841 gaatccaacc aagcgcttct cgcgcgggtc aaaaaggaaa tggatgagcc gcgcatggcc
      901 gaatccaccc gagaagagta tgacgtcaac cacaccacgc tgcgggagct gaaaaagctg
      961 tcgtaatccg tggtaaagag ggccgggact gtccacgcgc aacgggatac tccccaggaa
     1021 ggaaaacaat ggcaaagctc accaaggcag gtcgggatga gatatccgca gcaaacttcg
     1081 cgtttcccaa gcagcgcaag gagccgctgg agaacgcctc acacgtgcgc aacgcggttg
     1141 cgcgctttag tcaggtaaaa gacgtgacgg aagcagaacg tcaagcggcg tggaagcgaa
     1201 tacaggctgc cgccaagcta tacggtgtcg aactacaaca gtccaagtaa cgcgaacacg
     1261 ccacgcactg actatttatg agatagaggc tacgagagtt ggatcagact gccgtgtttc
     1321 aagcgcagac aaaatcaatg taaccatgct ggacatcagt ccgcgtcaat ttcgcgcgca
     1381 atttatcacc gacgtccaac ccctggtgcc cttgcgccaa tagtccttca atgtgaggtt
     1441 gcatgacacg aacaaacgtc ccgtgtggag tcacaccggt gacgatggca tcgaaagttt
     1501 cgccgatgcg ctggctcatg acgaccgctg ccagccgctt tgacatctcg cgctcaactt
     1561 ttcgagccgc atcttctttc aacgtgcagt tttttgcgat gtcggccaat tcatcatcgg
     1621 tatacggccc cgatgtccct gcaagaaagg tcttgaccaa cctttgcgtg acaacatccg
     1681 caaacctccg gtttggcgcc gtggagtgcg tgtagtcttg gaccgccagg ccgaagtgcc
     1741 cctgctcagg gtctccggga cgctccagca catactcgcc cggcccaatc agcttgatca
     1801 cagccaggga caagtccgca aagtgatcgg gatccgcgga cttgcgctta agcagaaaat
     1861 cgttgagtgc cttggaatcc ggttgcaccg gcaaggtttc accccgtgcc gccgcgagct
     1921 gaacgatccg gtcccaacgc tcaggagttt tcacgatgcg gcgcagcgaa gagacctttt
     1981 cgagaagtcg cgccacaacg ccgttggccc cgatcatgaa atcctcgatc aggtccgtgg
     2041 catggttcct cacctgtttt gcgatgtcca ccacctgctg gttcagcacg accggaagga
     2101 cttcattcgt atcaagattc aatgctccgt gaaggaagcg ctgtcttttc agcacttggg
     2161 cgacttcgtt ctgtaattta agctgctctt gcaaatcggt ggatgaggcc accttcggcg
     2221 gggccgccat cgtcccctca agccagctac ccacggcgtt gtacgtgagt tgtgccttgt
     2281 ttcgaacgag tgcccgatac accgtctctg agttcacatt g
//
LOCUS       DASRKK010000564        27267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1221643, whole genome shotgun sequence.
ACCESSION   DASRKK010000564 DASRKK010000000
VERSION     DASRKK010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 27267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27267
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221643"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            650..1879
                     /locus_tag="VFC15_13090"
     CDS             650..1879
                     /locus_tag="VFC15_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HZM11135.1"
                     /translation="MLTERLYYNDSSLLEFDATVTAVEEQGDQALVSLDRSAFYPTSG
                     GQNFDTGEIFGTANGSRVRVIDVQEQTGDILHLIDCPPGWLRPGVPVRGEVDADRRRD
                     HTQQHSGQHVLSAAFEKLYNFATVSFHMGDDLCTIDLAADAIHAKQLEAAERLANEII
                     AEDRPVEIRFATPEDARGMGVRKIPPAVREKLRLIDIRDFDLNACGGTHVHSTGQIGA
                     ILLRKTEKVKQGVRVEFVCGLRAVSTARHDFRTLTDAAAAFSTHLWEVPEQARKSLDE
                     IRSAQKAQHRLLEEVAELQAANLLRTADEKPIASGQKLVIQFFADRDLAFIKMLAQKL
                     IRLDKATVLLACGGAQPSLVFAQTPGLPNDMGALMKDTLQKLGTRGGGNRDMAQGGAP
                     DADRAEHALSEAASTIG"
     gene            2009..3355
                     /locus_tag="VFC15_13095"
     CDS             2009..3355
                     /locus_tag="VFC15_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZM11136.1"
                     /translation="MDSKLILVTVLIRLGVAAAISSVLVRARRFRVLLFREERNLGEK
                     IEIVLIIGIPIALGVLVRGWVRNFYAADISFEGAILMGVIGGRFVGTAGGILVSLPAL
                     LLGEWLALPMNILAGFLAGVLRHVAPEREAVWSFSPLFDLSIYHWIRRSVRKSLIDWQ
                     TSFFLLILALEFVRIQLHRAFPRRIFALDSNSWPVELAVYAGTVMAVAIALKVLNNVR
                     IEMKLEQQERLLLQTRMEALQSQINPHFLFNTLNSVSSLVRRDPDSAREMIVKLSNIL
                     RRLLRKGDSFVPLREEVEFVDDYLDIEVVRFGREKLKVVKELDPTSLDHLVPSMILQP
                     LVENAIKHGLADKLEGGHIYIRSRSSDSRIVIEVEDDGVGMPTSGTSSSSGTGIGMVN
                     ISERLQVVYGDAAEITIESQPDHGTLVRLVLPILLAEELGGSVAGALYEARSNTSR"
     gene            complement(3325..4473)
                     /gene="bshA"
                     /locus_tag="VFC15_13100"
     CDS             complement(3325..4473)
                     /gene="bshA"
                     /locus_tag="VFC15_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZM11137.1"
                     /translation="MKIGITCYPTYGGSGIVATELGLELAERGHEVHFISYSQPIRLT
                     GTQANIHYHEVEVSRYPLFEYPPYDLALATRMAEVAEIYDLDLLHVHYAIPHSVSAML
                     ARQMLACDHTRKHRRLPYVTTLHGTDITLVGADPSYLPITRFSIDQSDGVTSISQYLA
                     QRTIEVFEVATPIKVIPNFVNCDVYHRTPEIMQHRPEYAKREEKILVHLSNFRPVKRI
                     PDVMEIFDRVQQQMPARLLMIGDGPERSRAEWLALEKGIHGKIDFLGKSDRVHEILPL
                     ADVMLMPSQLESFGLAALEGMACQVVPIATDAGGVPEVIQHRKTGFLAAVGDVETMAK
                     YAVELLSDQEKLEDMGVAARASAQARFCATKIIPMYENYYREVLERAS"
     gene            complement(4864..5463)
                     /locus_tag="VFC15_13105"
     CDS             complement(4864..5463)
                     /locus_tag="VFC15_13105"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZM11138.1"
                     /translation="MNSGTRIPKFENPETGAERFGNLAGSDEERAQVLRATFGEQQHE
                     GVILASLDNVVNWVRKNSIWPMTFGLACCAIEMMSMGASRFDIARFGAEVFRPSPRQS
                     DLMIIAGRVSQKMAPVIRELYEQMPEPKWVISMGACATSGGVFNNYALVQGVNQWIPV
                     DVYVPGCPPRPEQLIYAIMLLQEKIKNDKGSFKRALNLG"
     gene            complement(5548..6108)
                     /locus_tag="VFC15_13110"
     CDS             complement(5548..6108)
                     /locus_tag="VFC15_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM11139.1"
                     /translation="MAEALSTFTIRVATLDDLTVLQGLVADSVWVLQAKEYNDAQREA
                     AIKLVYGIDTQLIRDGTYFVVEQDQQIVACGGWSRRRTLFGGDQHSPSRAPELLDSAN
                     EPARIRAFFVKPGWERRGIGSALVRACERAALSEGFHQIEMASTLTGVALYSAHGYRE
                     VERIDVPLEDELTLPVIRMAKAFAKS"
     gene            complement(6124..7146)
                     /locus_tag="VFC15_13115"
     CDS             complement(6124..7146)
                     /locus_tag="VFC15_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM11140.1"
                     /translation="MKAVRIHEFGGPEVLKYEDVPDPELRKDYVLVRIRACALNHLDL
                     WVRKGLPGVKLPHILGSDIAGEVVECGEYIDDLKPGTRVLLSPMLYCGHCEQCNSGRQ
                     NFCPQFAVLGNNAEGGNCELIAVPRANVIPIPDALGYTEAASVPLVFLTAWHMLVGNA
                     RLRAGQTVLVLGAGSGVGSAAIQICKMLGAEVIATAGDERKLAKARELGADHTIDHYK
                     QKISSEVKRITNKRGVDIVFEHVGTATWAESIKSLKYGGTLVTCGATTGFDATLDLRV
                     LFARQLSFHGSYMGGMGELDEVLKHVFAGKLKPVVDRTFPLSETRGAHEYLAGGEQFG
                     KVVVMP"
     gene            complement(7266..8084)
                     /locus_tag="VFC15_13120"
     CDS             complement(7266..8084)
                     /locus_tag="VFC15_13120"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZM11141.1"
                     /translation="MKNDFCFPTLFIPHGGGPCFFMDPPPDHPTLWDKMAAYLRGIDG
                     SLGRRPEAVLVISGHWDAAIPTVNSSIASPLLFDYFGFPEHTYRLTYPAPESPAVAAR
                     VRELLAKEGTSCDEDEHRGLDHGVFVPFKLIYPDADVPIVQLSLQRGLDPMTHLAIGR
                     ALAPLRKEGVLIVGSGMSYHNLQSFFVDDPRVNRPAEEFDEWLNDAVTALAGTSRADQ
                     LAEWQGAPGALAAHPTPEHLLPLMVAAGAAEGDWATRTYSDRLLGKPISGFQFG"
     gene            8234..9100
                     /locus_tag="VFC15_13125"
     CDS             8234..9100
                     /locus_tag="VFC15_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HZM11142.1"
                     /translation="MDIYEQIVQLRRDGRRGAVATITNVRGSIPSFQTAKMLVRDDGS
                     IVGTIGGGCVEAEVWQAAREVMEEEKPRSLTFNLNNNPKYDTGLVCGGTLDVFIEPVL
                     PPALLYIFGAGHVAFNLYKVAMIAGFDVIVVDDRESYANRERFPDAREVFADDFEKVI
                     AQIHVPESAYIVIVTRGHRDDMRVLRWAVNANTRYLGMIGSQRKTISIYKELEKEGIA
                     PEKFAHVHAPVGLEIGAVTPEEIAVAIVAEMIAVRRHTTPNVASKAFNYAAKHSAPED
                     SQDAVEETLGNL"
     gene            9162..9827
                     /locus_tag="VFC15_13130"
     CDS             9162..9827
                     /locus_tag="VFC15_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZM11143.1"
                     /translation="MPQTAGICGVILAAGESTRMGRDKALLPWPPVAEGAPAVNTFLG
                     AMIDLLQQHADLVIVVAGRNIAELEPIVDAHGAFTVVNPAPERGQFSSIQVGLRDVLN
                     RGRDAAFLVPVDRPPVLPATVRDLRAAFLAADTDVWAVTPEVLQDGAPVHGHPILIGR
                     EMMEQFLRAPAHSVARDIQHQHRQHIRYLRTQDTRVVANIDTPEDYQRLLRSEAVPAD
                     SAF"
     gene            9875..10537
                     /locus_tag="VFC15_13135"
     CDS             9875..10537
                     /locus_tag="VFC15_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HZM11144.1"
                     /translation="MALKPAIAVFDCDGTLWSGDSGRDFFYWEIERGLVSREVADAML
                     ARYRLYAAGEVGEEAMCGEMVTMHCSLSIDDLHRAAEEFFVEVVSHRVFPEMVGLTRQ
                     LARQGCELWAVSSTNNWVVEAGAAHFGIARDHVLAAAVHIDDGRATDRLHRVPTGELK
                     AVAIREVIGKPVHGVFGNSVHDHAMLEMAKHPFCINPSAELEQAAKEKNWTIYWPIGT
                     RK"
     gene            complement(10576..12864)
                     /locus_tag="VFC15_13140"
     CDS             complement(10576..12864)
                     /locus_tag="VFC15_13140"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZM11145.1"
                     /translation="MMSENGPKIRLVLLWHMHQPFYKDMVTGEYRLPWVRLHALKDYY
                     GMVKLVEEFPNVHQTFNLVPSLINQIQDYASGEARDPFLDLVSKPATELSASERRFAL
                     QYLFQANPVNMIGRHPRYRELLECFRATSNRPEGAESYFGTRDYADLQVLSQLAWFDE
                     YFLADADVAALIRKGEDFSRDDQLAMLAKQREILAKVVPAYAQAAKQGSVELSTTSYY
                     HPILPLICDTNVGAESTPGLPLPARRFRRPDDANQQIQRALESHQKTFGNRPLGMWPS
                     EGSVSEEVVALAHKQGMQWMATDEGVLERTIGYNFTRDGGGRLHGDSAEQLYNIHRYE
                     KGDTRMNMIFRDHTLSDLIGFVYSGMPAQDAANHLIHSIRESARPVLERGRDAVVSII
                     LDGENAWEYYPGSGREFLRRFYDGVQRDPEIEAVTVSEAIARHKESNFNRLNRLVPGS
                     WINANFNVWIGAPEDNKAWDLLSEARDYYDRHANRVSATQRRLAWEELLIAEGSDWNW
                     WYGPEHHSANDRDFDELYRKHLSNVYTALEGVPPEELTVPIASGVVRPSHHPQTAYVR
                     AKVDGFESGYFEWMGAASYTSDQRTSAMHGKQFLLDAVYAGLDADSVSGRLDFHKSLP
                     DGPYQLIVNFEVSRDGNDRNSPVDSYRLVVDAQGQNLLKWVLSNGNERNKLASYSSNN
                     GNTGTHGVEVAIGEIFEMRVPFELIGVKYGSRIRLRFAVWRDQLPADSLPVEGWIDVY
                     ALTEDELETSLHTYVPATKVRT"
     gene            13059..15443
                     /gene="lptF"
                     /locus_tag="VFC15_13145"
     CDS             13059..15443
                     /gene="lptF"
                     /locus_tag="VFC15_13145"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZM11146.1"
                     /translation="MRILTRYILKEVTSHALLGVLLFTFVIFMRDLGRLLELVVRNSA
                     PLPSVAEIFFYTLPTTFTITLPMGVLVGILIGLSRLAADSEVTAIRASGMGAGMFVRV
                     ISIFAVAAWALGMINSVYVAPKSATALSNLQNRLKSSQASFEIQPRVFYEEFKNNVLY
                     VQDAVPSKGQSLWRGVFLADVSDPASPKITLAERGALLSESPEKVRFHLEDGTQQELV
                     PKSKDQYSITTFDTTDIPIELPSASDHNPRDLLPVTELSLQGLLRNAQRERVAGAEVL
                     KIDPATSSSDFLKARYYEIEFHRRFALPAACLVLALVGIPLGLSAHKGGRSAGFVITI
                     VLVFIYYFFSLVGVSLARQGKVAPWLGVWGGNFFFFLCGLLLLWRVDRMPLDLSFLSS
                     AWTSLKKWASAISERLPGSPGSRRTRTRIRQPRKRFSARFPLILDDMILRDFATYLAM
                     ILATFLLLALVFTFFELLTDIVRNRISFVVLVEYLLNLSPSLIYLMAPVSVLLAVLIT
                     FGLLEKSSELTAMKATGFSIYRATLPIIVLSGVFAAGMFIFDQVYIPHTNKRQEILRN
                     QIKGKPPQTYLQADRKWIFGESNQIYYYQVFDPDVDRFGGITVFEFDPATFQLTRRIH
                     ADGSHWEPSLKKWVFENGWVRSLNGASIQEFRTFDVATFKELGEDPSYFKKEVRQSQE
                     MNYDQLRAYIEDLQHSGFDTVRLKVQLQKKLAFPLITLVMAVLAIPFAASGRRGGASA
                     EVAIAVGIAVVYWVTAGLFEAMGNANQLPAILAAWAPDLIFAFVGGYLLLRVPT"
     gene            complement(15467..16960)
                     /locus_tag="VFC15_13150"
     CDS             complement(15467..16960)
                     /locus_tag="VFC15_13150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM11147.1"
                     /translation="MSSSASEPRAGAVVGQRGSRVEAVQFRGWLPAILMVGAVLRLVE
                     LGRKSFWLDEITTVAIVRMPGFSFWSLVWHDEGNMALYYVLLRGWMHFGTSEATIRLL
                     SVIPGIATILLMYLLGTRLFGEKAGLLAAGFMAINACDVVVSQEARAYSFVVLGVAGS
                     TLLLVRCLERPSMARALAYGVVAGLTFYFHYFGILVPVAHAVSFAALPAARRHSKQML
                     VAAGIMAIFASPVLWMVHTQSIQHIEWVQRSSFLELYHFGSYLAAESGKVVGGVLLVL
                     DLILIGLFLRSYPRRGSDREDSLAPWRYALVASGFFTPVAISLMVSLIRPVFYHRFLI
                     ICLPFWILMMAVGVQQITARRWRAAAIGALCLLSLVATVLSYTRVKEDWRGVVGYLIN
                     HAQPEDRVLYYEAVGGYGAEGYRDWMEGPNTKRPTAIMIEPANVDWEKKTEQAPRVWL
                     VLFRAKPGDPGPASAIQNTLLGHFTAETPMRFRGITVIEYRAKPGAL"
     gene            17147..18382
                     /locus_tag="VFC15_13155"
     CDS             17147..18382
                     /locus_tag="VFC15_13155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM11148.1"
                     /translation="MVLVAFTVRVLWIALAHTYRLRTSENNLGFGWETGRIAYSLATG
                     HGFSSPFGGDTGPSAWTAPVYPWVVAFAFRTFGVYSRAAAFWMLVFNSFFAALTSWTI
                     YRTAQRVFGERVAVWSGWIWALLPNLVFWSVKFIWETSLSAFLLSLLFLLTLELEGED
                     RLSSWFAYGLLWGVMALTNPAALAFLPFAGTWLAYQLYRRGKRFLLPAATGAVIFWIT
                     IMPWLVRNDRVMGKPVFIRDNFGVEFRVGNNPLAEGINVGAIHPIENQLQFENYKRLG
                     EAAFAAEQGRLAREWVSQHPARFLTLSFRRFIFFWAGIPKPFTVEGLQEAKNLLFLAS
                     SLLAIGGLWLAWRHRVHGIFLFATLLVSYPMAYYITFPQPRYRHPIEPQLLILAVYLV
                     TEAKPRTDSDGIKRPEDNS"
     gene            18607..19602
                     /locus_tag="VFC15_13160"
     CDS             18607..19602
                     /locus_tag="VFC15_13160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM11149.1"
                     /translation="MQAVSVISTVLNEVAEVDALVDSLIGQSLVPAEIIIVDGGSNDG
                     TWEKLLAAKARCSALLPIRDESCSLKGSPGPIGRGRNVAIARASSDIIACADAGCVYV
                     PGWLESLTAPILSGNADYSLGGSWIDPNGRTVWDIASAPFLGISLTADAKTKSCTARS
                     MAFRKAVWETVGGFPETSFLFDDTGFDLSVRNIRKPAFVEDAKALYRPHLTLKTAVGQ
                     IARYGFADGVAGIRRPRFFRNLARCVALAAALLLLPKTAIPLVGVLLLECYFALRLDW
                     KSFLQVATPGSVAARLLYSLIVPWAVSWNYVAGAITKSNKPNAQNASLGEVRPQR"
     gene            19616..21412
                     /locus_tag="VFC15_13165"
     CDS             19616..21412
                     /locus_tag="VFC15_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM11150.1"
                     /translation="MFKNLRPLFPYLRKYRATFWIGALCVFCNNGVWILFPLVIGRAT
                     DDLNQGVTRHKLFTYSAMLLAVAGVKAIFQYLTRWILIGVSREIEFDLRNDLFTHLER
                     LSYSYYQRTRTGDIMARMTNDLNAVRMLVGPGIMYTANTVVFTVGALVFMFSISPKLT
                     IFAFAPLPIVSIVVQYFGRKIHERFERIQAMFSDISARAQENFSGARIIRAYVQEEAE
                     IALFEEANQEYIARSLKLVRLMGMLWPTLTTLLGVAIVIVLWVGGIEVIEHRITVGSF
                     VAFNTYMVQLTWPVIALGWVINIFQRGTASTLRIESILAESPEITNAGVPAFGAPTGI
                     VGEIEFRNLHFHYGQTLQASERGVGRKHSDENVLKNVSLKISAGTSLAIVGPTGSGKS
                     TLVSLIPRIYDAAPGSVFIDGRPIREFPLELLRKNIGFVPQETFLFSDSIRENIAFGA
                     LDATDADVRRVAEAANIASEIESFPEGYNTLVGERGLTLSGGQKQRTAIARALIRNPR
                     ILVLDDALSSVDTQTEDRILNHLREIMQGRTTIFISHRVSTVRNADRIAVLHDGHIVE
                     YGTHDQLIDRNGYYTELYNKQLLEEELETETV"
     gene            complement(21453..22019)
                     /locus_tag="VFC15_13170"
     CDS             complement(21453..22019)
                     /locus_tag="VFC15_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11151.1"
                     /translation="MFNRRLSFGLFALLLICRMAGFAQYNCIPSSTPPKLPAVRASGR
                     SSTAPLRVLQLAGIANSWKPTRREIGSVPAGTMVDVLEDVIVVDAPDIVRVTKPIEEL
                     NLKEGDEILRYAHLGEGWADFWVEGCSYKDANADFIIGPDGGGCAGTQCSARVTKLGR
                     QTWWFRIKLPNGKTGWTQSQNLDLSAGG"
     gene            complement(22012..22377)
                     /locus_tag="VFC15_13175"
     CDS             complement(22012..22377)
                     /locus_tag="VFC15_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZM11152.1"
                     /translation="MYSPQVLDHFEHPRNAGELAEADARVRVENPACGDILELSVKVV
                     DGRIAEVHFLAKGCVPAMACGSLITEMIAGNTVQQASAISREAVAKEIGGLPQASGHA
                     AQLAVDAVRALVEKMIPNV"
     gene            22449..23264
                     /locus_tag="VFC15_13180"
     CDS             22449..23264
                     /locus_tag="VFC15_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM11153.1"
                     /translation="MPSDYTPQRVVCLQPSATLTLRDLGLLDRVVACTKYCADVCPEA
                     ADGSRLIVSDSWTAQAEQIRTAQPDLVLASVPYQLEAVGEILKAGVRFLALAPRTLAD
                     IYADIVAIAGIMGVSERGEHVASAMQAAIEDLRMHCNSAEYKPRVFCEEWGKPIIASQ
                     TWVAELIDAAGGTLIAEPGKTTSAEEVAAAGPDVLLAAWCGAGDRVPLEKLVEQRNWS
                     DLPAVRARRVFCISDELLNTPATSLLGGLRAIAWALHPDRFPRPSGIRQLGDL"
     gene            23278..23760
                     /locus_tag="VFC15_13185"
     CDS             23278..23760
                     /locus_tag="VFC15_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM11154.1"
                     /translation="MPANYQTLADELTHLANAADSPEHLMTIIVERLQDVLTHYNWVG
                     FYMLEDGADGAEPMLVLGPYVGAATPHTRIPLHQGICGAAVSSAQTVVVDDVNSDPRY
                     LACSLETKSEIVAPIFVNGKVVGELDIDSHLPAAFKEDDRQLVEQCASLVGRYLEKAA
                     "
     gene            23804..24199
                     /locus_tag="VFC15_13190"
     CDS             23804..24199
                     /locus_tag="VFC15_13190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HZM11155.1"
                     /translation="MTKQVVAALLVRGDEILCCQRTEHQALALKWEFPGGKIEAGETP
                     QEALRRELDEELGINAEIGHEITRILHTYQNGNSVNLRFFVVERYQNELQNRIFREIR
                     WVKRGNLPTLDFLDADKELVHQLAAGELL"
     gene            complement(24324..25583)
                     /locus_tag="VFC15_13195"
     CDS             complement(24324..25583)
                     /locus_tag="VFC15_13195"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZM11156.1"
                     /translation="MSETRRLKVWRIRLLMFLAVIGPGFITANVDNDAGGIYTYSAAG
                     AQFGYLLLWVMIPMTIALVVVQEMSARMGAVTGKGLSDLIREEYGFRITFFMMLGLII
                     TNFGNIVAEFAGIASSLELFHISRYISVPAAGLVVWLMVVKGTYKSVEKVFLAASFFY
                     FAYIIAGVLAGPSWREAFVATFKPPHSSAFHNSAYIYMIIAVVGTTIAPWMQFYLQSS
                     IVEKGITAREYRASRWDVILGCLFTDIVAWFIVVACAATLYAHGVRQIRDAADAAQAL
                     RPLSNEFAYILFAAGLFNASIFAASILPISTAYTVCEGLGFESGVGKKFAEAKVFYWL
                     YTLLLIAGGAVILIPNIPLVKISILSQVVNGIVLPFVLIFMLLLVNKSELMGKHINGT
                     WFNAIAWATTIIMIGLTIAWFVTGGGS"
     gene            complement(25693..26937)
                     /locus_tag="VFC15_13200"
     CDS             complement(25693..26937)
                     /locus_tag="VFC15_13200"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZM11157.1"
                     /translation="MPSLALSDLIGAPVYDPSGTSPVGKVREVAILPQEDPNRISGFV
                     VKTRQGERLLAIKSVESVNGGVRADSMFADWAPYASGEGMLLLERDLLDQQIIDVYGR
                     KVVRVNDVDIHEELGHDHIVLKLAAVDVGARGAMRRLLRGVIPGAALRSMMERIPPRM
                     IPWEFVDLIETDPARRVKLKISLDRLAKLHPADIADIVEELAPTEREAVFETLDDEVA
                     ALTLEEIDPKMQVSILSSLDSDRAADIVEEMDPDAAADLLGDMPPETTQEILTEMQPE
                     ERAEVTELMTFEEDTAAGRMTTEYMALKAGAKVSDAIEMLRNFEGGLESMSTIYIIGE
                     REKLLGQVPLAKIALATHDTPLMMLSSSHMPTIHPGADEKEVAELFDKYNLLTLPVID
                     DDGVLTGVITADDVINMLRAKL"
BASE COUNT         5607 a         8667 c         7240 g         5753 t
ORIGIN      
        1 cctgctaagc gaggtaggag caaaatggcg gaagccgaag aacaatcacg cgaagcgcgg
       61 cggcgcaagg ctgtggaaaa gccgctccgt ggaaaagtcc acaaaacgga ctttcccact
      121 acgcttggaa atccgcacac atcgcggatt tccactttac gcacagcctc aactactact
      181 gcacaataaa aatgcacgac cacgacaaaa ggggacattt ctaatgtctt aaacaagggg
      241 acatttctaa tgtccgttga caatccgtga ctaactcgct tgccgtttct cactgctcct
      301 cataaactct ctccggaagg atgagggaga gctgtcgtcc aagttcaaaa gagtcggcag
      361 ccggatactt cccgagtccc caataggtgg ttcgacctct gatgggtcac atttgttctc
      421 gctttttcac tcctccgcct gctgacgccg tcgtccagcc gctagaaatc tcgcctaagt
      481 ccttattctc tgctactttg cctataaccg ctttgtttcc actactttgc accggaaaca
      541 cctcctatcc tcaacaaaac aaggattggg gagggggagg gtacccctgt gcagactaga
      601 gaacacggtt acactgtgtc gtgatgttct cgacacgctc aggaccctca tgctgaccga
      661 gcgcctgtat tacaacgatt cctcgctgct cgagttcgac gccaccgtga ccgccgttga
      721 agagcagggt gatcaagctc tcgtttccct ggatcgctca gctttctatc ccaccagcgg
      781 cggccagaac ttcgacactg gcgagatctt tgggactgcc aacggctctc gcgtacgggt
      841 catcgacgtt caggagcaga ccggcgacat cttgcacctt attgactgcc ctcccggttg
      901 gctgcgtccc ggggtaccag ttcgtggcga agttgacgcc gatcgccgcc gtgaccacac
      961 gcagcagcat tccggacagc atgtcctctc cgctgccttt gagaagctct acaacttcgc
     1021 gaccgtctcc ttccacatgg gcgacgacct ctgcaccatc gaccttgccg ccgatgcgat
     1081 ccacgcgaag caactggagg ctgccgaaag actggcgaac gagatcatcg ccgaagatcg
     1141 accggttgag attcgctttg ccactcccga ggatgcgcga ggcatgggcg tgcgcaagat
     1201 cccacccgca gtgcgggaaa agctgcgcct catcgacatt cgcgactttg acctgaatgc
     1261 gtgcgggggc acccacgtcc acagcaccgg ccaaatcggt gccattcttc tgcgcaaaac
     1321 agaaaaggtc aaacagggtg tgcgcgtcga gttcgtctgc gggctccgag ccgtcagcac
     1381 agctcgccac gacttccgga ctcttacgga tgctgccgcg gccttctcca cccacttgtg
     1441 ggaggtgccc gagcaggcgc gcaagtcgct cgacgagatc aggtccgcgc agaaggcgca
     1501 gcatcgcctg ctggaagaag tcgctgagtt gcaggccgcg aatctgcttc gcaccgccga
     1561 tgaaaagccc attgccagcg gccagaaact ggtcattcaa tttttcgccg accgcgatct
     1621 tgcgtttatc aagatgctcg cgcaaaagct catccgcctc gacaaagcaa ccgttcttct
     1681 cgcctgcggt ggcgcacagc cttccctggt tttcgcgcag actcccggtc ttcccaacga
     1741 catgggagcg ctgatgaaag ataccctgca gaagttggga acgcgcggtg ggggcaatcg
     1801 cgacatggca caaggtggcg ctcccgatgc cgaccgcgcc gaacatgccc tcagcgaagc
     1861 cgccagcacg ataggataga agcgtgtgag agagtttttc gccaaagagt tcacggagca
     1921 catgaagagg aagatgaaaa agaccagatg gaactccgtg ttctccgtgc ctccgtggtg
     1981 aattcgtctc tcccgcgata ggtaaatttt ggattccaaa ctcatcctcg tgaccgtact
     2041 gatccgcctc ggcgtggccg cggcgatcag cagcgtgttg gtgcgcgccc gtcgctttcg
     2101 cgtgctcctg ttccgcgaag aacgcaacct gggcgagaag atcgaaattg tcctgatcat
     2161 cggtatcccc atcgccctcg gagtcctggt gcgcggctgg gtccgcaact tctacgcggc
     2221 ggacatctcg ttcgaaggcg ccatcctaat gggagtaatc ggcgggcgat tcgtcggcac
     2281 cgccggtggc atcctcgtct ctcttcccgc gcttttgctc ggcgaatggc tcgcgctccc
     2341 catgaatatc ctggccggtt tcctcgccgg cgtgctccgc cacgttgccc ccgagcgcga
     2401 agctgtctgg tcgttttctc cgctgttcga cctaagcatc taccactgga ttcgccgcag
     2461 cgttcgcaaa tcactcatcg attggcagac ttcctttttt ctgttgatcc tcgcgctcga
     2521 gtttgttcgc atccagttgc atcgcgcctt tccgcgccgc atcttcgcac tcgatagcaa
     2581 cagttggccg gtcgaactcg cggtatatgc aggaaccgta atggccgtgg ccatcgccct
     2641 caaggtcctc aacaacgtcc gcatcgaaat gaagctcgag caacaggagc gcttgctgct
     2701 gcagacgcgc atggaggccc tgcaaagcca gatcaatccc cacttcctgt tcaacactct
     2761 gaattccgtg tcctcgcttg tccgccgcga tcctgactcg gcgcgcgaaa tgatcgtcaa
     2821 gctcagcaac atcctgcgac gtcttttgcg caaaggcgat tcgtttgtcc cgttgcgcga
     2881 ggaagtcgaa ttcgtggacg actatctcga catcgaagtc gtccgcttcg gtcgtgaaaa
     2941 gctcaaagtg gtcaaggaac tcgaccccac ttccctcgac catctggtgc ccagcatgat
     3001 tctgcaaccc ttggtcgaga acgccattaa gcatggtctt gccgacaaac tcgagggtgg
     3061 ccacatttac attcgcagcc gctcgtccga tagccgcatc gtgatcgaag tcgaggatga
     3121 cggagtcggt atgccaacct caggaaccag tagctcctca ggaacgggta ttggcatggt
     3181 caacatctct gagcgtctgc aggttgtgta cggagacgcc gccgagatca cgatcgaaag
     3241 ccagcccgac cacggaacgc tggtgcgcct ggtgctccca atcttgctgg ctgaggagtt
     3301 gggcggctca gtcgccggag cgctctacga agcccgctcc aacacttcgc gatagtaatt
     3361 ctcgtacatc ggaatgatct ttgtggcgca gaatcgtgcc tgcgcgctcg cacgcgccgc
     3421 gacgcccatg tcttccagct tctcctggtc cgacagaagt tcgacggcgt atttcgccat
     3481 cgtctccaca tctcccactg ccgcgaggaa cccggttttg cgatgctgaa ttacttccgg
     3541 cactccgcct gcatcggtgg cgatgggcac cacctgacac gccattcctt ccagcgccgc
     3601 cagtccgaac gactccagtt ggctgggcat cagcatcacg tccgccagcg ggagtatctc
     3661 gtgcacccgg tctgattttc ccaggaaatc gattttgccg tggattccct tctcgagcgc
     3721 cagccactca gcgcgtgatc gctccggacc atccccaatc atgagcaatc gcgccggcat
     3781 ctgttgctgt acgcgatcaa aaatctccat gacatccgga atgcgcttca ccggacggaa
     3841 attcgacagg tgcaccagga tcttctcctc tcgtttggca tactccggac ggtgctgcat
     3901 gatctccgga gtacggtggt acacgtcgca attcacgaag ttcggtatca ccttgatcgg
     3961 cgtcgccacc tcgaacacct cgatcgttcg ctgggccagg tactgcgata tcgaggtgac
     4021 cccgtcgctc tggtcgatgg agaaccgcgt gatcggcaga tacgacggat cggctcccac
     4081 cagcgtgatg tcggtcccat gcagcgtggt gacataagga agcctgcggt gcttgcgggt
     4141 gtgatcacag gcaagcattt gccgcgccag catcgcgctc accgaatgcg gaatcgcgta
     4201 gtgcacgtgc agcaagtcga gatcgtaaat ctccgcaact tccgccatgc gcgtggccag
     4261 cgccaggtcg tacggcggat attcgaacag cggataacgc gacacttcca cttcgtgata
     4321 gtggatattg gcttgcgtcc cggtaagccg gatgggttgg gaatacgaga tgaaatgcac
     4381 ctcgtggccg cgctccgcca actccaaccc cagttccgtc gccacaattc cactaccacc
     4441 ataggtggga tagcacgtga ttccaatttt catttttata aactcgcaat cattgtgacg
     4501 taccgcgcgc gattgcgata gctcgtccgt cgcatcattg aggtttcaac tccagcgatt
     4561 ggatggctct cccgggattg agattcacgg gtcgacgttc tgacttgcca tcagccttcg
     4621 gacttgccat cagcctttgt atttgtcatc ggcttttgga tttgtcatca cgtcgcagcg
     4681 agggatctgc tctttctttc cgccgtcatc ccgagcgaag tgaggtcgcc gcagcgaccg
     4741 agcgcagtcg aaggacctgc ttttcgcttc tccccgcgac aatcccaatc ttcacgcagg
     4801 tctaagccca gacaacaaaa acgcccgtcg aagcgggctt tgaaacgaaa tcgaactgaa
     4861 agtctatccc aggtttaacg cacgcttgaa cgagcccttg tcgttcttga tcttttcctg
     4921 cagcagcatg atggcataaa tcagctgttc gggacgcggc ggacatcccg gcacatagac
     4981 gtccacggga atccactggt tcacaccctg caccagcgcg tagttattga acaccccacc
     5041 cgacgtcgcg cacgcgccca tggagatcac ccacttgggc tcgggcatct gctcataaag
     5101 ctcgcgaatc accggcgcca tcttctgcga cacccgtccg gcaatgatca tcaggtccga
     5161 ctgccgcggc gagggacgaa acacctccgc gccgaatcgt gctatgtcga aacgcgacgc
     5221 gcccatcgac atcatctcaa tcgcgcaaca ggcgaggcca aacgtcatag gccagatgga
     5281 atttttccgc acccagttca cgacgttatc gagagaggcc agtatcacgc cttcgtgctg
     5341 ctgctcgcca aaggtcgcgc gcaacacctg cgcacgttcc tcgtccgatc ctgccaggtt
     5401 gccgaaccgc tcggctcccg tctccgggtt ctcgaatttg gggatacgcg ttccggaatt
     5461 catagtggca ttatacccat tctgttggac gctccgccgg ccacgcaaga tgcacgccgg
     5521 ctcccggccg aaagctttgc aggcggatta cgacttggcg aatgccttcg ccattcgaat
     5581 taccggcagt gtcagctcgt cctccagagg aacatcgatc ctctcgactt cccggtagcc
     5641 gtgcgcggaa tacagcgcga cgccagtcaa agtggaagcc atttcgatct ggtgaaatcc
     5701 ttcagacaat gcagcccgct cacaggcgcg aaccagcgca ctgccgatgc cgcggcgctc
     5761 ccagccgggt ttcacgaaaa aagcacgaat tcgcgccggt tcgttcgcgg aatcaagcaa
     5821 ttcgggcgcc cggctcggac tatgctggtc cccgccgaac aaagtccggc gacggctcca
     5881 gcccccgcac gccacaatct gctggtcctg ctcgaccaca aagtatgtgc catcccgaat
     5941 cagttgcgtg tcaatcccgt acaccagctt gatcgcagcc tcgcgctgcg cgtcgttgta
     6001 ctccttggct tgcaggaccc acaccgagtc cgcgaccaaa ccctggagaa cggttaggtc
     6061 atccaaggta gcgacgcgaa tcgtaaatgt ggaaagcgcc tcggccatct ctgcgcgctc
     6121 gatctacggc atcacaacga ctttcccaaa ttgctccccg cccgccagat actcatgcgc
     6181 accccgcgtc tcgctcagcg gaaatgtgcg gtccactacc ggcttcagct tgccggcgaa
     6241 cacgtgcttc aacacctcgt ccaactcgcc catgcctccc atatagctgc cgtgaaacga
     6301 tagttgccgc gcaaacagca cccgcagatc gagagtggcg tcgaatcccg tggtcgcgcc
     6361 gcaggtaacc agcgttccgc catatttaag cgacttgatg ctctccgccc aggtagctgt
     6421 tccaacatgc tcgaagacaa tatcgacccc gcgtttattg gtaatgcgct tcacttcgct
     6481 ggagatcttc tgcttgtaat gatcgatggt gtggtcggcc ccgagttcgc gggccttcgc
     6541 cagtttgcgc tcatcgcccg ccgtcgcgat cacctccgct cccagcattt tgcaaatctg
     6601 gatggctgcg cttcccacac ctgatcccgc cccaagtacc agcaccgttt gtcccgcccg
     6661 cagccgcgca ttgcccacca gcatgtgcca ggccgtcagg aacaccagcg gcaccgaagc
     6721 cgcctccgtg taaccgagtg catcgggaat tggaatgaca ttcgccctcg gaactgcgat
     6781 cagttcgcag ttgccgccct cggcgttatt gcccaacacg gcgaattggg gacagaaatt
     6841 ctgccgcccg ctgttgcact gttcgcaatg accgcaatac agcatcggag acagcagcac
     6901 gcgcgtaccc ggctttagat cgtcgatgta ctcgccacac tccaccacct cgcccgcgat
     6961 gtcgctgccc agaatgtgcg gcagcttgac cccaggcaac cccttgcgca cccaaagatc
     7021 aagatggttc agcgcgcatg cgcgaatgcg caccagcacg tagtctttgc gcaactccgg
     7081 atcgggaacg tcttcgtact tgagtacctc gggaccaccg aattcatgaa tgcgaaccgc
     7141 tttcatcacg cgctccttgc gtcgccggca gcgttggact gggaactcaa aactctactg
     7201 tcttccacta ccgctccgca agggtcgcga actggatgct cccgatcgaa cggaggagca
     7261 gggtcctagc cgaactgaaa ccccgatatc ggtttcccca gcaacctgtc gctataggtg
     7321 cgcgtcgccc aatccccctc ggcggcccca gcggctacca tgagtggcaa cagatgctcc
     7381 ggcgtcggat gcgctgcgag cgctcccggt gctccctgcc actcagcgag ttgatccgcg
     7441 cgactcgtcc cggccaatgc ggtgaccgca tcgttcagcc actcgtcaaa ctcttctgcc
     7501 ggacggttga cgcgcgggtc atccacaaag aagctctgca ggttgtgata gctcatgcca
     7561 ctccccacga tgagcacgcc ttccttccgc agcggagcca gtgctcgccc gatggcaaga
     7621 tgtgtcatcg gatcgagacc gcgctgcagc gaaagctgca cgatcggcac gtcggcgtcc
     7681 ggataaatca gtttgaacgg aacgaacacg ccgtgatcca aaccgcgatg ctcgtcctca
     7741 tcgcaggatg tcccttcttt cgccagcaat tctcgaaccc gcgccgccac tgcgggcgat
     7801 tcgggcgcgg gataggtgag ccggtacgtg tgttccggaa aaccgaagta atcgaacagc
     7861 aaaggagacg cgatactgct attcacggtt ggaatcgccg catcccaatg ccctgagatg
     7921 accagtaccg cctcgggccg gcgtccgagc gaaccatcga ttccgcgcag atacgcagcc
     7981 atcttgtccc agagcgtggg atggtctggc ggcggatcca tgaagaaaca cgggccgcca
     8041 ccatgcggaa tgaacagcgt gggaaaacaa aagtcatttt tcatgagaat gcacagtcag
     8101 atggcacaag tcgctcgacg gttgctgttt cgccgatcac ttgcgccttg ctccccaagc
     8161 ttatctgcag ccgcctgaca ctcccgcttt gacttcgccg cctgcacccc taataattac
     8221 caaagcctga tcaatggaca tctacgaaca aatcgtgcag ctacgccgcg atggacgtcg
     8281 tggcgccgtt gccaccatca ccaacgtgcg tggttcgatc ccgtcgttcc agactgcgaa
     8341 gatgttggta cgcgatgatg gatcgatcgt cggcaccatc ggcggcggtt gtgtcgaggc
     8401 cgaagtctgg caggctgccc gcgaggtgat ggaggaagag aagccgcgct cgcttacctt
     8461 caacctgaac aacaatccca agtacgacac cggcctggtt tgcggcggca cgctcgacgt
     8521 gttcattgag cccgtgctcc cgcccgccct gctctacatc ttcggtgccg gtcacgtggc
     8581 cttcaacttg tacaaggtcg cgatgatcgc cggctttgac gtgatcgtgg tggacgaccg
     8641 cgaaagctac gctaaccgtg agcgcttccc tgacgcacgc gaggtcttcg ccgacgactt
     8701 cgaaaaagtc atagctcaaa tccacgtgcc cgagtccgcc tacatcgtga tcgtcacccg
     8761 cggacaccgc gacgacatgc gcgtgctgcg ctgggccgtg aatgccaaca cgcgctatct
     8821 cggcatgatc ggctcgcaac gcaaaaccat ctcgatttac aaagagttgg aaaaggaagg
     8881 catcgcgcct gaaaagttcg cccacgttca cgctcccgtc ggactcgaaa tcggagcggt
     8941 gacgccggaa gaaattgcag ttgccatcgt cgccgaaatg atcgccgtgc gccgccacac
     9001 cacgccgaat gtagccagca aagccttcaa ctacgccgcc aaacactccg ctcccgaaga
     9061 ctcccaggac gcagtggaag agactttggg caacctctag ccccacaacc tcaaccgtcg
     9121 cacaactgac acgcatccct tcccttcggt atcatcaagc catgccgcaa accgctggta
     9181 tctgcggagt catcctcgcc gcaggcgaat cgacccgtat gggccgcgac aaagcgttac
     9241 ttccctggcc gccggtggct gaaggcgcgc ccgccgtcaa cacgtttctg ggtgccatga
     9301 tcgacctgct gcagcagcac gcggacttgg tgatcgtggt cgcgggcaga aatatcgccg
     9361 aactcgagcc catcgtcgac gctcatggcg cgtttaccgt cgtcaatcct gctcccgagc
     9421 ggggacagtt cagctccatc caagtcggct tgcgcgacgt gctcaaccgt ggccgcgacg
     9481 ctgcgttcct ggtcccagtg gatcgtccgc ctgtgctgcc ggcaacggtt cgcgatctac
     9541 gcgcagcgtt tctggcggcg gataccgacg tctgggccgt gacgccggag gtcttgcagg
     9601 atggagcccc agtgcacggg catcccattc tgattggccg cgagatgatg gagcagtttt
     9661 tgcgtgctcc cgcccattca gtcgcgcgcg atatccaaca ccagcatcgg cagcacatcc
     9721 gctatttacg cacgcaggat acccgggtcg tcgccaacat cgacacgccg gaagactacc
     9781 agcgcctcct gcgttcggaa gccgtccccg cagattccgc gttttgactt catccgatat
     9841 gtccgattcc gcttcagaat tcatagcatc agtcttggcc ctgaaacccg ctatcgcagt
     9901 cttcgattgc gacggcacct tatggagcgg tgattccggc cgcgactttt tctactggga
     9961 gatcgaacgc ggcctggtca gccgtgaggt cgcagacgcc atgcttgcgc gttaccggct
    10021 ttacgcggcg ggggaagtgg gcgaagaggc aatgtgcggc gagatggtca ccatgcattg
    10081 cagcctctcg attgatgacc tgcatcgggc ggcagaagaa ttctttgtcg aagtcgtgag
    10141 ccatcgcgtg ttcccagaaa tggttggact cacgcgccaa ctcgccagac aaggctgcga
    10201 actctgggcg gtatcatcca ccaacaactg ggtagtggaa gccggagcgg cgcattttgg
    10261 cattgcgcgc gaccacgtgc tggcggctgc cgtccacatc gacgatggcc gcgccactga
    10321 ccgtcttcac cgcgtaccca ccggcgaact caaggcggtg gcgattcgcg aagtgattgg
    10381 caaaccggtg cacggagtct tcggcaactc ggtgcacgat cacgccatgc tggaaatggc
    10441 gaaacatccc ttctgcatca accccagtgc tgaactggag caggccgcaa aagagaaaaa
    10501 ctggaccatc tattggccaa tcggcacccg caagtagcta agcggagcgc ctgatttacg
    10561 cttgagaatg agggattagg tccgcacctt cgtcgcgggc acgtacgtat gcaggctggt
    10621 ctccaactcg tcttcggtga gtgcgtagac atcgatccag ccttccaccg gcagcgagtc
    10681 agccggtaat tgatcgcgcc acaccgcaaa ccgcagcctg attcggctgc catactttac
    10741 gccgatcagc tcaaacggca cccgcatttc gaagatttcc ccgattgcga cctcaactcc
    10801 gtgtgtcccg gtgttcccgt tgttcgacga atagctggcg agcttgttcc gctcattgcc
    10861 attgctcaag acccacttca gcaggttctg cccctgggca tcgaccacca ggcgatagga
    10921 atcgactggc gaattgcggt cgttgccatc ccggctgacc tcgaagttca caatcaactg
    10981 atacggccca tccggcaggc tcttgtgaaa gtccagccgt cccgagaccg agtcagcgtc
    11041 gagtcccgca taaaccgcgt ccagcaggaa ttgcttgcca tgcatcgcac tggtgcgctg
    11101 gtccgacgta tagctcgcag cgcccatcca ctcgaagtag ccgctttcga agccgtccac
    11161 tttggcccgg acatacgcag tctgcggatg gtggctcgga cgcaccactc cgctcgcgat
    11221 ggggaccgtc aattcttccg gcggtacgcc ttccagtgcg gtgtacacgt tggagagatg
    11281 cttccggtaa agttcgtcga agtcgcgatc gttggccgag tgatgctccg gtccatacca
    11341 ccagttccaa tcgctgcctt ccgcgatcag caactcttcc cacgccagtc tgcgttgggt
    11401 ggccgaaacg cgattggcgt ggcgatcgta atagtcccgc gcctcggaca gcaggtccca
    11461 cgccttgtta tcttcggggg cgccgatcca gacattgaaa ttagcgttga tccacgaccc
    11521 cggcaccaac cggttcaggc ggttgaaatt cgactctttg tggcgcgcaa tggcctcgct
    11581 caccgtcacc gcttcaatct cgggatcgcg ttgcacacca tcgtagaagc ggcgcaggaa
    11641 ctcgcggccc gatcccggat agtattccca cgcattttcg ccgtccagaa tgatcgacac
    11701 cacggcatcc cggccgcgtt ccagcaccgg tcgcgccgac tccctgatgc tgtggatgag
    11761 gtggttcgca gcgtcctgcg caggcattcc cgaatacacg aatccgatca agtccgaaag
    11821 ggtgtgatcg cggaagatca tgttcatccg cgtgtcgccc ttttcgtagc gatggatgtt
    11881 atacagttgc tcggcgctgt cgccgtgcaa tcgtccgccc ccatcgcgcg tgaagttgta
    11941 accgatcgtg cgctccagca cgccttcgtc ggtcgccatc cactgcatgc cctgcttgtg
    12001 cgccagagcg accacttctt ccgagacgct gccctccgac ggccacattc ccaggggacg
    12061 attcccgaac gttttctggt gcgactcgag ggcacgctga atttgctggt tcgcgtcatc
    12121 gggccgtcga aagcggcgcg ccgggagcgg caacccaggc gtggactccg ccccaacgtt
    12181 ggtgtcgcaa atcagcggca aaatcgggtg gtaataggac gtagtagaaa gctcgaccga
    12241 accttgtttt gccgcttgcg cataggctgg cacaaccttc gccagtatct ctcgctgctt
    12301 cgccagcatt gccagctgat catcgcggct gaaatcttca cccttccgga taagcgcggc
    12361 aacgtcggcg tccgccagaa aatattcatc gaaccacgcc agttgcgaca gcacttgcag
    12421 gtcggcgtaa tcccgggtcc cgaagtacga ttccgccccc tccggacggt tgctcgttgc
    12481 ccggaagcac tccagcaact cccgataccg cgggtggcgt ccaatcatgt tgacggggtt
    12541 ggcttggaaa agatactgca acgcgaaacg gcgctcggaa gccgaaagct cggtggctgg
    12601 ttttgaaacc agatcgagga acgggtcgcg cgcctctccc gatgcgtagt cttgaatctg
    12661 gttaatcagc gacggaacca ggttgaacgt ttggtggacg ttgggaaact cttccaccag
    12721 tttcaccatc ccgtagtagt ccttcagggc atgcaaccga acccagggaa ggcgatactc
    12781 gccggtcacc atgtccttat agaaaggttg gtgcatgtgc cagagcagca ccaaccgaat
    12841 tttcggcccg ttctcgctca tcattgcctg atttgcggaa tcagtgtaac aggggattca
    12901 cacagtttgg aaccgcgaac atgttccgaa atgacacgat ggtgggagaa ttcgatcaca
    12961 accgtgtacc tacacgcaaa ggggctagcg caatcaggca cttacggggc acacgcaaaa
    13021 cttacacccg ccctgcactt ctattacact tacagtttgt gcgcattctc acccgctaca
    13081 tcctgaagga agtgacttct cacgccctgc tcggcgtgtt gctcttcact ttcgtcatct
    13141 tcatgcggga cctcggacgt ctgctggagt tggtcgttcg caacagcgct cccctgccca
    13201 gcgttgccga aattttcttt tacacgctcc ccaccacttt caccatcacg ctgcctatgg
    13261 gcgtattggt cggcatcctg atcggattga gccggctcgc cgccgatagc gaagtcaccg
    13321 ccattcgcgc cagcggcatg ggtgccggga tgttcgttcg cgtcatttcg atattcgccg
    13381 tcgccgcatg ggccctggga atgattaaca gcgtctatgt cgctccaaaa tcggcgaccg
    13441 cgctcagcaa tttgcaaaat cgcttgaaga gctcccaggc gtccttcgaa attcagcccc
    13501 gtgtcttcta cgaagagttc aagaataacg ttctgtacgt gcaggacgct gtaccgtcaa
    13561 agggtcaatc gttgtggcgc ggcgtctttc tggccgacgt cagcgatcct gcttctccca
    13621 aaatcacgtt ggcggagcgc ggcgccctgc tcagcgaatc tcccgagaag gtccggttcc
    13681 atcttgagga cggcacccag caggagttgg taccaaagtc aaaagaccag tactcgatta
    13741 ccacgttcga caccaccgac attccaatcg agctgccttc ggcgtcggac cataacccca
    13801 gagacttgct cccggtgacg gagttgagcc ttcaaggcct gttgcggaac gcgcagcgcg
    13861 agcgcgtcgc cggcgccgaa gttctgaaaa tcgatcccgc tacttcgtcc tccgacttct
    13921 taaaggcgcg ctattacgaa attgaatttc atcgccgttt cgcgctgccg gcagcctgcc
    13981 tggtgctcgc gttggtcgga attccgcttg ggctctctgc acacaaaggc gggaggagcg
    14041 caggattcgt tatcaccatc gtgctggttt tcatctatta cttcttttcc ctggtgggcg
    14101 tctcattggc gcgccaaggc aaggttgcgc cctggctggg tgtatggggg ggcaacttct
    14161 tcttcttcct ttgcggactc ctgctccttt ggcgcgtcga tcgtatgccg ctcgatctca
    14221 gttttctcag ctccgcatgg acgtcgctga agaagtgggc gagtgcgatc agcgagcgtc
    14281 tacccggttc cccaggcagt cgtaggacgc gcacccgcat tcgtcagccc aggaaacgat
    14341 tcagcgctag gtttcccctg atcctcgacg acatgatcct gcgtgatttc gccacctacc
    14401 tggcgatgat cctcgctacc tttctgctct tggcgctggt cttcacattc ttcgaattgc
    14461 tcaccgacat tgtccgcaac agaatatcgt tcgtcgtgct cgtcgaatac ctgctgaacc
    14521 tgagcccctc gctgatttat ctgatggccc ccgtgtcggt tttgctggcg gtgctgatca
    14581 ccttcggcct cctggagaaa tccagtgaac tcacggctat gaaggccacg ggcttcagca
    14641 tttatcgtgc gacgcttcct atcatcgttc tatccggagt ctttgccgct ggtatgttca
    14701 tttttgacca ggtctatatc ccgcatacca acaagcgcca ggaaatcttg cgcaaccaga
    14761 tcaagggcaa gcccccgcag acttatctgc aagccgaccg caaatggatt ttcggcgaga
    14821 gcaaccagat ttactactac caggtgtttg atcccgacgt tgaccgcttc ggcggaatca
    14881 ccgtcttcga gtttgaccca gccactttcc agttgacgcg ccgcatccac gctgacggtt
    14941 cgcactggga accaagcttg aaaaagtggg tgttcgagaa cggctgggtg cgttcgctca
    15001 acggcgcttc catccaggaa ttccgtacct tcgacgttgc aacatttaaa gagctgggcg
    15061 aagatccgag ctacttcaag aaggaagtgc ggcaatccca ggagatgaat tacgaccaac
    15121 tccgcgccta catcgaagac ctgcagcaca gcggtttcga cactgtacgc ctgaaggtgc
    15181 agctgcagaa aaaattggct ttccccttaa tcaccctggt gatggcagtc ctggctattc
    15241 ccttcgccgc gtccggacgt cgtggcggag cgtcggcgga agtcgcgatt gccgtcggta
    15301 tagccgttgt ctattgggtc accgcaggat tgttcgaagc catgggcaac gcgaatcagc
    15361 ttcccgccat cctggccgcc tgggcgccgg atttgatttt cgccttcgtg ggcggctatc
    15421 tgttgctgcg agtgcccacc taaggcggtc tgcgtgatcc tgccgtttat aacgctccag
    15481 gctttgctcg atattcgatg accgtaatcc cgcgaaatct catcggagtt tcagccgtga
    15541 agtgtcctag caacgtgttc tgtatcgcac tggcaggccc cggatctccc ggtttggccc
    15601 gaaacaatac cagccacact cggggtgctt gctccgtctt cttctcccaa tcgacgttgg
    15661 ccggttcaat catgatcgcc gtcggacgct ttgtgttcgg cccttccatc cagtcgcgat
    15721 aaccctcggc cccataacca cccacggctt cgtaatagag gacccggtcc tcgggctgtg
    15781 catggttgat taaataacca accaccccgc gccaatcttc cttcacccgc gtgtacgaga
    15841 gcacagtcgc caccagtgac agtaggcaga gtgccccaat cgctgccgct ctccatcgcc
    15901 tcgcagtgat ttgctggacg cccactgcca tcatcagtat ccaaaacggc agacagatga
    15961 tcaggaatcg atgatagaaa accggcctga tcaacgacac catcagactg attgcaaccg
    16021 gagtgaagaa cccactggcg actaacgcgt atcgccatgg cgctaggctg tcttccctgt
    16081 cgctcccgcg cctcgggtac gatctcagga atagaccaat caaaatcagg tccaaaacca
    16141 gcaacacgcc gccgacgacc ttcccacttt cggcagccag ataactcccg aagtgataca
    16201 gctccaagaa cgatgaccgt tgcacccatt cgatgtgctg gatactctgg gtatgaacca
    16261 tccacaaaac cggcgacgca aatattgcca tgattccggc ggcaaccagc atctgcttcg
    16321 agtgtcttcg ggccgccgga agtgcggcga acgacacggc gtgcgccact ggaaccaata
    16381 tcccgaagta gtgaaagtaa aaagtcagcc cggcaaccac gccgtaggcc agggcccgcg
    16441 ccatcgaagg gcgctccagg cagcgcacca acagcaacgt cgaacccgcc accccgagca
    16501 ccacaaagct gtaggcccgc gcttcctgcg agaccacgac gtcgcacgca ttgatggcca
    16561 tgaaccctgc ggccagcaat cccgcctttt ccccaaacag ccgggtgccc agcaggtaca
    16621 tcaacaggat tgtcgcgatc ccaggaatta cactcagcaa tcgaatggtt gcttcgctgg
    16681 ttccgaagtg catccatccg cgaagcaaaa cgtagtacag cgccatgttg ccttcgtcat
    16741 gccagaccaa cgaccagaat gaaaacccgg gcatgcggac gatggcaacc gtggtgattt
    16801 catccagcca gaaactctta cggcctagtt ccaccagccg aaggaccgca ccaaccatca
    16861 ggatcgcagg caaccacccg cggaattgca ccgcctcgac gcggctgccg cgctgtccca
    16921 ccaccgcccc tgctcggggt tcggatgctg aagaagacat aatttttcaa tatagcagtt
    16981 cgaacgcggg gcgccgatcc gcagcaacgg cccgatccca tgcgatacaa ttcgcgttgt
    17041 ctccatgcct tccaatatcc aaccggcaga atcgccctta gttcctcaga cctccgccgc
    17101 cggttctggc tcgcgatggg cgcatattct tcgctctccg cgatacatgg tgctggttgc
    17161 gtttacggtg cgcgtgctgt ggatcgcctt agcccatacc tatcgcctgc gtacttccga
    17221 aaataacctg ggcttcggtt gggaaactgg ccgtatcgcc tattcccttg ccaccggtca
    17281 tggcttcagt tcaccatttg gtggagacac cggcccctcc gcgtggacgg cgcctgttta
    17341 tccctgggtg gtggcctttg cctttcggac tttcggtgta tattcccgcg ccgccgcttt
    17401 ttggatgttg gtctttaata gtttcttcgc tgcccttacc agttggacaa tctaccgcac
    17461 cgcacaacga gtcttcggcg agcgagtggc cgtttggtca ggttggattt gggccctcct
    17521 tcccaacctt gttttctggt cggtcaaatt catctgggag accagccttt cggcattcct
    17581 tctgagtttg ctgttcctgt tgaccctcga gttggagggt gaggatcggc tctcttcctg
    17641 gtttgcctat ggcttgcttt ggggcgtcat ggcattaact aatccggcag cgttggcgtt
    17701 tcttcccttc gccggtacct ggttggccta tcaactgtat cgtcgaggaa agcgatttct
    17761 gctgcccgcc gccactggcg cagtgatctt ttggatcacc atcatgccct ggctggttcg
    17821 caacgaccgc gtgatgggca aacctgtctt cattcgcgac aacttcggag tcgagttccg
    17881 cgttggcaac aatcctttgg cagagggcat caatgtaggc gctatccacc ccatcgagaa
    17941 tcaacttcag ttcgagaact ataagcgtct gggcgaagcc gcttttgccg ctgagcaagg
    18001 acgactggcc agggaatggg tgtcacaaca tcctgccaga ttcctcaccc tcagctttcg
    18061 ccgctttatc ttcttctggg ccggaatacc caagcccttc actgtagaag gtctccagga
    18121 agctaagaac ctgctattcc tggcttcatc gctgctggca attggtggcc tgtggcttgc
    18181 atggaggcat cgggttcacg gtatatttct ctttgctact ttgctcgtta gttatcctat
    18241 ggcatattac ataacctttc cacagccacg ataccgccat cctatagaac cgcaactcct
    18301 gattcttgca gtctatctgg taaccgaagc taaaccccgc accgacagtg acggcattaa
    18361 gcggccggag gataacagct agtctgaagc ctgttattcg aagtaaggat atatttattg
    18421 ctacatctcc attagccgag cgatgctcgt gtaactatgc aagagcttca cactatcttc
    18481 gccaccggca agagcaggaa agagggagca tcgccgaccg tcgcgaagtc tgcctccttt
    18541 gctttctttc ctcttccggc gaatgctcgc tggccaccca ctcgacgtgc ggagtatcat
    18601 ctaatcatgc aagcggtaag tgtcatttcc acagttctca atgaagtcgc cgaagtagat
    18661 gctctggttg acagcctcat aggacagtca ctggtcccgg cagaaatcat catcgtcgac
    18721 ggcggctcca atgacggtac gtgggaaaaa ttgctggctg cgaaggcgcg atgctctgcc
    18781 ctgcttccga ttcgcgatga gagttgtagc ttgaaggggt ccccaggccc catcggccgc
    18841 ggccgaaacg tcgccatcgc gcgagcgtcg tccgacatta tcgcgtgcgc agatgcaggt
    18901 tgcgtgtatg ttcctggatg gctggaaagc ttgaccgctc caattctttc cggaaacgcc
    18961 gactattcgc tcggtgggtc ttggatcgac cccaatggtc ggaccgtctg ggatattgcg
    19021 tccgcgccct tcctcggaat ttccttgacc gccgatgcca aaaccaaatc ttgcactgct
    19081 cgctccatgg cgtttcgcaa ggcggtatgg gagaccgtag gaggttttcc tgagaccagc
    19141 tttctctttg atgacacggg attcgatctg agtgtccgca acattcgcaa gccggcgttc
    19201 gtcgaagatg ccaaggcact ttaccggcct cacctcaccc tgaaaaccgc tgtcggccag
    19261 atcgcccgct atggatttgc cgatggcgtc gccggaatca ggcggcctcg tttttttaga
    19321 aacctggcta ggtgtgtcgc cctggcggcg gctcttttgc ttctcccaaa aaccgcgatt
    19381 ccccttgtag gcgtgctgct gctcgaatgc tattttgccc tgcggctcga ttggaagagc
    19441 ttcttgcagg tagccactcc gggctcggtg gccgctcgac tcctctactc gcttatcgtg
    19501 ccgtgggcgg tgtcgtggaa ttacgtcgca ggcgccatca caaagtccaa taagccgaac
    19561 gcgcaaaacg ccagcttggg tgaagtccgc ccgcaacgct gatcgctgac tgcctatgtt
    19621 caagaacctg cgcccgcttt tcccgtatct ccgcaaatac cgagccacct tctggatcgg
    19681 cgctctctgc gtgttttgca acaacggtgt ctggatcttg ttccccctgg tgatcggccg
    19741 cgccaccgac gacctcaacc agggcgtcac ccgccacaag ctattcacct attcggcaat
    19801 gctgctcgcg gttgcgggcg tcaaggccat ctttcagtat ttgacccgat ggatcctcat
    19861 cggcgtttcg cgcgagatcg aattcgacct gcgcaacgac ctgttcaccc acttggagag
    19921 gctttcctac agctactatc agcgcacccg aaccggcgac atcatggccc gcatgaccaa
    19981 cgacttgaat gccgtgcgca tgctggttgg tcccggaatc atgtacacgg ctaacaccgt
    20041 ggtgttcact gttggagcgc tggttttcat gttcagcatc agccccaagc tgaccatctt
    20101 cgccttcgcg ccgttgccca tcgtcagcat cgtagtccaa tacttcggac gcaaaatcca
    20161 cgaacgcttc gaacgcattc aggcgatgtt ttccgatatc tccgcccgcg cccaggaaaa
    20221 cttctccggc gcgcgcatca tccgcgccta cgttcaggaa gaggcggaga tcgccttgtt
    20281 cgaagaagct aaccaggaat acatcgcccg cagcctcaaa ctggttcgcc tcatgggaat
    20341 gctctggccc acgctcacca cgctgctcgg tgtggccatc gtcatcgtgc tctgggtcgg
    20401 cgggattgaa gttatcgagc accgcatcac cgtcggcagc ttcgtggcct tcaacaccta
    20461 catggtgcaa ctgacctggc ccgtgatcgc gctgggatgg gtcatcaaca tcttccagcg
    20521 cggcaccgcc tcgactctgc gcatcgagtc gatcctcgcc gagtcgcccg agatcaccaa
    20581 tgcaggagtg cccgcattcg gcgcgcccac tggcatagtc ggagagatcg agttccgcaa
    20641 cttgcacttc cactacggcc agacactgca ggcgtcggag cgtggagtgg gacgcaaaca
    20701 tagcgatgaa aacgtcctga aaaatgtgag cttgaagatt tccgccggca ccagcctcgc
    20761 catcgtcgga cccactggct cgggaaaatc caccttggtc tcgctcattc cgcgtatcta
    20821 cgacgccgcg cccggttcgg tgttcatcga tggacgcccc atccgcgaat tccctctcga
    20881 gctacttcgc aagaatatag gttttgtgcc gcaggaaact tttctattca gcgacagcat
    20941 ccgcgaaaac attgccttcg gcgcgctcga cgccaccgac gccgatgtgc gccgcgtcgc
    21001 cgaggctgcc aacatcgcca gtgagatcga aagctttccc gagggataca acacgttggt
    21061 tggcgaacgc ggtctgaccc tttccggcgg acaaaaacag cgtactgcta tcgcgcgcgc
    21121 cttgattcgc aatccccgca tcctggtgct tgacgacgcg ctctccagcg tcgacaccca
    21181 aaccgaagac cgcatcctca atcacctgcg agaaatcatg cagggccgca ccaccatctt
    21241 catctcgcat cgcgtgtcca cggtccgcaa tgccgatcgc attgccgtgc ttcacgacgg
    21301 acacatcgtc gagtacggca cccacgatca gctcatcgac cgcaacggct actacaccga
    21361 gctctacaac aagcagttgt tggaagaaga gttggaaacg gagacggtat aggcggcagg
    21421 cagaagtcta tacgggttgt gcccgcaacg gattatccgc ctgccgacaa atcgagattt
    21481 tggctttgcg tccagcctgt ttttccgttg ggaagcttaa ttcgaaacca ccaagtctgc
    21541 cttcccagct tcgtcactct tgccgagcat tgagtacctg cgcagcctcc accgtcaggc
    21601 ccgatgatga agtcggcgtt cgcatcttta tacgagcagc cctctaccca gaagtcggcc
    21661 caaccttccc ctagatgcgc gtagcgaaga atctcgtcgc cttccttgag attcaactcc
    21721 tcgatgggct tcgtcacccg cacaatatca ggcgcgtcga cgacgatcac atcctccaac
    21781 acatcgacca tcgtgcccgc tggaaccgac ccgatctcac ggcgcgtcgg tttccagcta
    21841 ttggcgatgc ccgccaactg cagaacccgt agcggagcgg tcgaagagcg tccactcgcg
    21901 cgcaccgccg gaagcttcgg gggggttgag ctgggtatgc agttgtactg ggcaaagccc
    21961 gccattcgac agatcagcaa caatgcgaaa agtccgaagc tcaagcggcg gttaaacatt
    22021 cgggatcatc ttctccacca gcgccctcac cgcatcgacc gccaactgcg ccgcatgccc
    22081 cgacgcttga ggtagcccac caatttcctt cgccacagcc tctcgactga tcgcactggc
    22141 ttgctgaacg gtgttcccgg caatcatttc agtgatcaat gaaccacacg ccattgcagg
    22201 cacacatccc ttggcgagaa agtgaacttc agcgatgcga ccgtcaacca ccttgaccga
    22261 cagctcgaga atgtcaccgc aagccgggtt ctccacccgt acccgtgcat cagcctcggc
    22321 gagttcgccc gcgttacggg gatgttcgaa atggtcgagc acttgaggtg aatacattct
    22381 gttttgcaga gtaccgcatc cttcgattca cataaaatcg actcaccctt cgtataatcg
    22441 atcaactcat gccctcggac tacactccgc aacgcgtcgt ctgtctgcaa cccagcgcta
    22501 ctctcaccct ccgcgacctc ggattactgg atcgcgttgt cgcctgcaca aaatactgcg
    22561 cggacgtgtg tcctgaagcc gcagacggct cgcgcctcat cgtctccgat tcgtggactg
    22621 cgcaggccga gcagattcga acagcccagc ctgatctcgt cctcgcctcc gtgccctatc
    22681 aactcgaagc cgtgggagaa atcctgaagg cgggcgtgag attcttggcc ctggctcctc
    22741 gaaccctcgc cgatatctat gccgacatcg tcgccatcgc tggcatcatg ggcgtctccg
    22801 agcgtggcga acacgtcgcc tcggcgatgc aggccgccat cgaagacctg cggatgcatt
    22861 gcaattcagc cgaatacaag cctcgagtct tctgcgaaga atggggcaag ccgatcatcg
    22921 cttcccagac ttgggtggcc gaattgattg acgccgccgg tggtactctc attgccgaac
    22981 ccggaaagac gacatctgcc gaagaggtcg ccgccgcggg tcccgatgtc cttctcgcag
    23041 cttggtgcgg cgcaggcgac cgcgttcctt tagagaagct tgtcgaacaa cgcaactgga
    23101 gcgacctgcc agccgtgcga gcacgacgcg tgttttgcat cagcgacgag ttgcttaata
    23161 ctccggcgac cagtttgctt ggcgggttgc gcgccatagc gtgggcgctg caccctgatc
    23221 gcttccccag accatctgga atcaggcaac tcggcgacct gtgatttaat gaggctcatg
    23281 cccgcgaact atcagactct ggcggatgaa ctcacccatc tggcgaatgc cgccgacagc
    23341 cccgaacatc tcatgaccat tatcgttgag cgactgcaag acgtgctgac gcactacaac
    23401 tgggtcggct tctacatgct cgaggacggc gcagacggtg ccgagcccat gctcgttctc
    23461 ggcccttatg taggagccgc aactccccac acgcgcatcc cgttgcatca ggggatctgt
    23521 ggggccgccg tttcctccgc tcaaactgtg gtcgtcgacg acgtgaacag cgatccccgc
    23581 tatctcgcct gttcgctcga aaccaagtct gagatcgtcg ccccgatttt tgtgaacggt
    23641 aaggtagtcg gagaactcga tatcgacagt catctcccag ccgccttcaa agaagacgac
    23701 cgccaactcg tcgagcagtg cgccagcctc gtcggacgat atctggaaaa ggccgcatga
    23761 tggctctcca ccgcgccgca cccgtgcaaa cgaggaacgc atcttgacca agcaagtggt
    23821 ggccgccctg ctcgtgcgcg gtgacgaaat tctgtgttgc cagcggaccg agcaccaggc
    23881 cctcgcgctg aaatgggagt tccccggagg caaaattgaa gcaggtgaaa cccctcaaga
    23941 ggcattgcgc cgcgaactcg acgaagagtt ggggatcaac gcggaaatag gacacgagat
    24001 cacgcggatc ctgcacactt atcagaatgg taactcggtc aacctgcgct tctttgttgt
    24061 cgagcgctac cagaatgaat tgcagaaccg tatcttccgc gaaattcggt gggtaaagcg
    24121 cggaaacctg ccgacgttgg atttcctcga cgccgacaaa gaactagtcc accagctcgc
    24181 agccggagag ctcctctaga actgtctcgg aattgttgca agtgtagaac tgcagtttag
    24241 cggcggcaac gactctgcgt ttcgacttga gtcggtttca accgaatctc taaccactaa
    24301 ccactatcca ctatccactg ttcttagctt cccccgcccg tgacaaacca cgcaatcgtc
    24361 agtccgatca tgatgatcgt ggtcgcccac gcaatcgcat tgaaccacgt gccattgatg
    24421 tgtttcccca ttaactcact cttgttcacc agcagcaaca tgaagatcag cacaaacggc
    24481 agcacgatgc cgttcaccac ctgtgacaga atgctgatct tcaccagcgg aatattcggg
    24541 atcaggatca ccgctccgcc tgcgatcagc agcagcgtgt acagccagta gaagactttg
    24601 gcttcggcga acttcttccc cactcccgac tcaaagccca gtccttcgca aaccgtgtac
    24661 gccgtcgaaa tcggcagaat tgaagcggcg aatatcgacg cgttgaataa gcccgcggcg
    24721 aacaggatgt acgcaaactc attcgataac ggccgcaacg cctgcgcggc gtcggcggca
    24781 tcgcgaatct gccgaacgcc atgcgcatac agcgtcgcag cgcacgccac cacaatgaac
    24841 catgcgacga tgtcggtgaa caggcagccc agaatcacgt cccagcgcga agccctgtat
    24901 tctctcgccg taattccctt ctcgacaatc gacgactgca gatagaactg catccacggc
    24961 gcaatcgtgg tccccaccac cgcaatgatc atgtagatgt aggcggaatt gtggaacgcc
    25021 gacgagtgcg gaggcttaaa cgtcgcaaca aacgcttccc gccagcttgg gcccgccagg
    25081 acgcccgcaa tgatgtaggc gaagtagaaa aatgacgctg cgagaaacac cttctcgaca
    25141 ctcttatatg ttcccttcac caccatcagc cacaccacca gccccgccgc cggaacgctg
    25201 atgtaccgcg agatgtggaa caactccagg ctgctggcga tgcccgcaaa ctcggcaaca
    25261 atgttgccaa agttggtgat gatcaacccc agcatcatga aaaacgtgat gcggaacccg
    25321 tactcttcgc ggatgaggtc gctcagtccc ttgccagtca ccgcgcccat gcgcgctgac
    25381 atctcctgca ccaccaccag cgcgatcgtc atcggaatca tcacccacag cagcaggtac
    25441 ccgaactgcg ctcccgcggc ggagtaggtg tagattccac cggcatcgtt gtcgacgttc
    25501 gccgtgatga accccggccc gattacggcc aggaacatca gcaggcgtat tctccagact
    25561 ttcagacgtc gcgtttcgga catgacaccg gaacttgagg cgagatggtg aagccgttcc
    25621 agaataactg tctgggatga tatgcgccaa ctcggggatg aatattgcgg aaatacgccg
    25681 gtgggatcga ctctacagct tggcgcgcag catgttgatc acgtcatcag ccgtgatcac
    25741 gccagtcaga acgccgtcgt cgtcgatcac cggaagcgtc agcaggttgt acttgtcgaa
    25801 caactcagca acttctttct cgtcggctcc aggatgaatc gtcggcatat gactcgagct
    25861 cagcatcatc agcggcgtgt catgggtcgc cagcgcaatc ttcgccagcg gaacctgccc
    25921 cagcagcttc tcgcgctcgc cgatgatgta gatcgtgctc atgctttcca agccgccttc
    25981 aaagttgcgc agcatctcaa tcgcatccga caccttggcg cctgccttca gcgccatgta
    26041 ctcggtggtc atgcgccccg cagccgtgtc ctcctcgaag gtcatcagct cggtgacctc
    26101 ggcgcgctct tccggctgca tctcggtcag aatttcctgc gtcgtctccg gaggcatatc
    26161 gcctaacaga tcggccgccg cgtccggatc catctcctcg acaatgtccg ccgcgcggtc
    26221 cgaatccagc gagctgagga tcgacacctg catcttggga tcgatttcct ccagcgtcaa
    26281 cgcggcgact tcatcatcca gcgtctcgaa caccgcctcg cgctccgtcg gagccagttc
    26341 ctccacaatg tcggcaatgt ccgccggatg cagtttcgcc aaccggtcca gcgagatctt
    26401 cagcttcacc cgccgcgccg gatcggtctc aatcaggtcc acaaactccc acggaatcat
    26461 ccgcggcgga atccgctcca tcatcgaccg caacgccgcc cccggaatca ctccgcgcaa
    26521 cagccgccgc atcgccccgc gcgcgcccac atcaaccgcg gccagcttca gcacaatgtg
    26581 gtcatgcccc agctcttcgt ggatatcgac gtcattcacc cgcacaacct tgcggccgta
    26641 cacgtcgatg atctgctgat cgagcagatc gcgttccagc agcagcatgc cttcgccgct
    26701 cgcatacggc gcccaatcgg cgaacatgct gtcggcgcgc acgcctccat tcaccgactc
    26761 caccgactta atcgccagca gccgttcgcc ctgccgcgtc ttcaccacga accccgatat
    26821 gcggttggga tcttcctgcg gcaatatcgc gacctcgcgc acttttccca cgggcgaagt
    26881 gcccgacgga tcatacacag gcgcgccaat caggtccgaa agtgcaagcg aaggcatcac
    26941 tgccaatata gcggaatcac cgtacagaaa tttagttgtt ttgcagagtc acggcttctc
    27001 ttcattccgg tatcgcagcc ggattcagag tctgctggct aacaaggctt ggtttgcgat
    27061 ggtcgtgtga atcaacagaa gtccagcccc ggaggggcga catagtctag cccagcactt
    27121 agccggacgg gggtccccga tccgcgccgc atttgcgcgc atcggggtgg gcaggcggag
    27181 tgctgggcta gagtcgtaaa aatattccga gtcccggagg gacggcagat gttccgaggg
    27241 ggcttcagcc ccgagacccc ccttccc
//
LOCUS       DASRKK010000565         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_342491, whole genome shotgun sequence.
ACCESSION   DASRKK010000565 DASRKK010000000
VERSION     DASRKK010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342491"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFC15_13205"
     CDS             <1..791
                     /locus_tag="VFC15_13205"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZM11158.1"
                     /translation="TDALIERKRRQIELLQEKRSALISHAVTKGLDSHAPMKDSGIEW
                     LGQIPKHWRIVPLKYSLLPRGGAIKTGPFGSQLLSSDMASGDVKVYNQRNVLDRDFLS
                     GENYVSDDKYEELKSFTVFPQDVLITTRGTIGRCALLPDNAERGILHPCLMRVQPNPL
                     ITSPEYVALLIQDSGIVLLQLQLLSNATTIEVIYSESLKSVRLPLPPASEQQAILTYV
                     TREAERHDALISKVQDSIDVLREHRTTLISAAVTGKIDVRGEVA"
     gene            791..1858
                     /locus_tag="VFC15_13210"
     CDS             791..1858
                     /locus_tag="VFC15_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZM11159.1"
                     /translation="MARRGLQPKFTITNAITAALTRIERARGFLEAATLSEDWVRAMG
                     ERALVLEAHHTTHIEGTHLTLDQAERLLAGKAVPEADPDDARELLNYRRAFEFVSEYL
                     DSGAPITEGLVREIHKRLVEGVRGGSATPGQYRKVQNYVVNGATGQTVYTPPPAADVP
                     ALMAEMVTWLNAPDQDGPGVHPVLVSGAAQFQLVHIHPFLDGNGRTSRLLSTLCLYRA
                     GYDFKRLFTISEYYDRNRAAFYAAIQGVRDGGMDLTGWLEYFVEGLATQLAEVSERGK
                     KAMKADLVARQHELNERQTKALSFLLDHDEMHIRDLEGLCPDVNRRTLQRDLQQMEDM
                     GLVGRKGAARKSLYILKNKGL"
     gene            1950..5468
                     /locus_tag="VFC15_13215"
     CDS             1950..5468
                     /locus_tag="VFC15_13215"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HZM11160.1"
                     /translation="MPGQHTEHAFETAIEHHLTTAGGYVKGDRDGFDPHRAIFPASVL
                     AFIKATQPDEWAYLEGIQKTKASETLLDDLCRALDSEHEGCLSVLRHGFKCFGKLFHV
                     AYFAPASGMNPETKKRYEANRLTITRQLRYSPKHGNTLDVTLALNGIPVATAELKNPM
                     TAQTWRHAVTQYKNDRDASDLIFQFKHRALVHFAVDTDEVYMTTRLSGAKTTFLPFNK
                     GCAGGGGNPDNPGGYKTAYLWEEVLERHSFLDILARFIHLQIEEKKLGDKKVKRQTMI
                     FPRYHQLDCVREMVDDARQRGVGNNYLVQHSAGSGKSNSIAWLAHRLSSLYDEQDEKV
                     FDSVIVVTDRVVLDQQLQNTIYQFEHKQGVVEKIEVNSTQLADALAAGTPIIITTLQK
                     FPFVTEKIGELPKKRYAVIVDEAHSSQGGETATELKGVLAGAAIKEEAKAKAEAEGLP
                     DYEEEILKAMAKRGKQPNISFFAFTATPKYKTLEVFGRPGPDDKPLPFHLYSMRQAID
                     EGFILDVLQNYTTYKTYYRLIKSIEDDPKVDKRKAARALARFMSLHPHNIAQKTEVMI
                     EHFRHFTLHKIGGRAKAMVVTSSRLHAVRYKQAFDKYITEKGYTGIKTLVAFSGTVID
                     PDAPDVEYTESGMNIDAQDKHIGQKELPERFATEEYQVLLVAEKYQTGFDQPLLHTMY
                     VDKRLAGIQAVQTLSRLNRTHPGKEDTFVLDFVNEPDEILAAFQPYYEQTLIGEQADP
                     KQLYELHAKLDAQQVYHKAEVEEFCKIFYKPKRNQTAADHARMNACIDPAVGRFNELE
                     EEEREEFRKTLTAFRNLYSFLSQVIPFQDTDLEKLYSYIRFLLTKLPRADYGAVYHFS
                     DDVSLKYYRLQKIGEGSIDLEPGKTEPVDGPTAVGTKAAHPDDIELSRLIDILNERFG
                     TEFKPGDELFFESIREDAVADSNLRQAAMANTMENFGYVFHKALEGLFIDRMDQNEEI
                     TARYMNEEQFRKAVGQHLLKDVYEQVRSEQARPPTPAQEPFRRVESREEDRYRTCVPL
                     YTLKAAAGEFGDGQQVEPDGWVVPKVPRQLSEGMFVAQVVGRSMEPRIPDGAWCLFRS
                     PVSGSRQGRIVLVQHRSIHDPETGGSYTVKRYESEKEADGEGGWRHQVIRLRSENPEF
                     DPITLKGVTEGEVSVIAELVQVLSAT"
     gene            5608..5835
                     /locus_tag="VFC15_13220"
     CDS             5608..5835
                     /locus_tag="VFC15_13220"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZM11161.1"
                     /translation="MNPFCTFMASAAGRIIRIIVGAVLIAWGLLSLGGTVGIIVAVVG
                     LVPFLAGLLDFCVFAPLFGCPWAGAQIRAGK"
     gene            complement(5841..>6215)
                     /locus_tag="VFC15_13225"
     CDS             complement(5841..>6215)
                     /locus_tag="VFC15_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11162.1"
                     /translation="PRFVNVHRTVGGPNEVGSTVRYDVLLSRLSFNVVLEKVVAGRYL
                     LYRVQGGFPRGGVLTFDIDRSRPGGGFLTIYVAFNFPTSRNPLKRLGWYLFKLAFPGY
                     VHDVIWNHSLCELKHVAELDQE"
BASE COUNT         1354 a         1848 c         1949 g         1064 t
ORIGIN      
        1 ggaccgatgc gttgatcgag aggaagcggc ggcagatcga gttgctccag gagaagcgat
       61 ccgccctcat cagccacgcc gtcaccaagg gcctcgactc ccacgccccc atgaaagact
      121 ccggcatcga atggctgggc cagattccga agcactggcg gatcgtccca ctcaagtact
      181 ccttgcttcc tcgaggaggg gcaatcaaga cgggtccgtt cggtagccag ttgctttcgt
      241 ctgacatggc gagcggcgat gtcaaagtct acaaccagcg caacgtcctg gatcgagact
      301 tcttatccgg ggagaactac gtcagcgatg acaaatacga agagctgaag tcgtttacgg
      361 tcttcccaca ggacgttttg attacaacga gagggaccat cggccgctgt gctttacttc
      421 cagacaatgc ggaaagaggc atactgcatc cctgccttat gcgggtccag cccaacccgc
      481 tcattacatc gcccgagtac gtcgcccttc ttattcagga cagtggcatc gtcctcctgc
      541 aacttcagct gctgagcaac gcaacgacta tcgaggttat ctactctgaa tcgttgaagt
      601 cggtacggct cccgctacca cccgcctccg agcaacaggc catcttgact tacgtcacgc
      661 gggaggcaga acgccacgac gcattgataa gtaaggtcca agacagcata gacgttctgc
      721 gggagcatcg cacaaccctg atctccgcag cggtgaccgg caagatcgac gttcgagggg
      781 aggtggcgtg atggccaggc gcgggttgca gccgaagttc acgatcacca atgccatcac
      841 ggcggcgctg acgcggatcg agcgcgcacg gggcttcctg gaagccgcga cgctgtcgga
      901 ggactgggtt cgggcgatgg gtgaacgggc gctggtgctc gaagcgcacc acacgacgca
      961 catcgagggg acgcacctga cgctggacca ggccgagcgg ctgctggcgg gcaaggcagt
     1021 gcccgaggcg gaccctgacg acgcccgcga actgctgaac tatcgccggg cgttcgagtt
     1081 cgtctcggag tatctggaca gcggcgcacc gatcaccgag gggctggtgc gtgagattca
     1141 taagcgcctg gtcgagggcg tccgcggcgg cagcgccacg ccgggccaat accgcaaggt
     1201 gcaaaactac gtggtcaacg gtgcaaccgg ccagacggtc tataccccgc cgcccgccgc
     1261 agacgtaccg gcgctgatgg cggagatggt gacgtggctt aatgcgcccg accaggatgg
     1321 tccgggagtg catccggtgc tggtcagcgg cgcggcgcag ttccaacttg tacacattca
     1381 tccgtttctg gacggcaacg ggcggacgtc gcggctgctc tcgacgctgt gcctgtatcg
     1441 ggccgggtac gacttcaagc ggcttttcac gatcagcgag tactacgacc gcaaccgagc
     1501 ggcgttctac gcggcgatcc agggcgtgcg cgatggcgga atggacctga ccggctggct
     1561 ggaatacttc gtcgaggggc tggccacgca actggccgag gtctccgagc ggggtaagaa
     1621 ggcgatgaag gccgatctgg tcgcgcggca gcacgagttg aacgagcggc agacgaaggc
     1681 cctgagtttc cttctcgatc acgacgagat gcatatccgg gacctggaag gcctgtgccc
     1741 ggacgtgaac agacggactc tgcagcggga cttgcagcag atggaggata tgggccttgt
     1801 tgggcgaaaa ggcgcggcca ggaagtcgct ttacatcctg aaaaacaagg gcttatgagt
     1861 tatcacgaca tatcgcgaca gagaatcgcg acgaatcgcg acatggatgg cgacaaactc
     1921 gcgacacgac ttgcgccgga ggacgcctga tgccgggcca gcataccgag cacgccttcg
     1981 agaccgccat tgagcaccat ctgacgacgg cgggcgggta cgtcaagggc gaccgcgacg
     2041 gcttcgatcc gcatcgggcg atcttcccgg ccagcgtgtt ggcgttcatc aaggcgacgc
     2101 agccggatga gtgggcctac cttgaaggca tccagaagac taaggcctct gaaacgttgc
     2161 tggatgacct atgccgggca ttggactcgg agcacgaagg gtgcctttcg gtgttgcggc
     2221 acgggttcaa gtgctttggc aagctgtttc acgtggcgta cttcgccccg gccagtggga
     2281 tgaacccgga gacgaagaag cggtatgagg cgaatcgcct cacaatcacg cggcaactgc
     2341 gttactcgcc gaaacacggc aacacattgg acgtgaccct tgcgctcaac ggcatcccgg
     2401 tggcgacggc ggaactgaag aaccccatga ccgcccagac ctggcggcac gcggtcacgc
     2461 agtacaagaa cgaccgcgac gcgtcggacc tgatcttcca gttcaagcat cgggcgctgg
     2521 tgcatttcgc cgtggacacc gacgaggtct atatgaccac gcggttatcg ggcgccaaga
     2581 ccaccttcct gccgttcaac aagggctgcg ccggtggtgg cgggaacccg gacaacccag
     2641 gcggctacaa gacggcctac ctgtgggaag aggtgctgga gcggcatagc ttcctggaca
     2701 tcctggcccg cttcatccac ctgcagatcg aggagaagaa gctcggcgac aagaaggtca
     2761 agaggcagac gatgatcttc ccccgctacc accagctcga ctgcgtgcgg gagatggtgg
     2821 atgacgcacg gcagcgcggc gtcgggaaca actacctcgt gcagcactcg gcgggcagcg
     2881 gcaaaagcaa ctccatcgcc tggctggcgc accggctgag cagcctgtac gacgagcagg
     2941 acgagaaggt gttcgattcg gtcatcgtgg tgaccgaccg ggtcgtgctg gatcagcagc
     3001 ttcagaacac gatctaccag ttcgagcaca agcaaggcgt ggtggagaag atcgaggtca
     3061 actcgaccca gttggccgat gcgctggctg ccgggacgcc aatcatcatc accacgctgc
     3121 agaagttccc gttcgtgacc gagaagatcg gcgaactgcc caagaagcgg tatgccgtga
     3181 tcgtggatga agcccatagc tcacagggcg gcgagacggc gacggaactc aagggtgtgc
     3241 tggcgggcgc ggcgatcaaa gaggaggcga aggccaaggc cgaggcagaa ggcctgcctg
     3301 actacgagga agagattctc aaggcgatgg ccaagcgcgg gaagcagccg aacatcagtt
     3361 tcttcgcctt cacagccacg cccaagtaca agacgctgga ggtcttcggc cggccggggc
     3421 cggatgacaa gcctctgccc ttccatctct acagcatgcg acaggccatc gacgagggct
     3481 tcatcctcga cgtgctgcag aactacacca cgtataagac ctactaccgc ctgatcaagt
     3541 ccatcgagga cgaccccaag gtggacaagc gcaaagcggc gcgggcgctg gcccggttca
     3601 tgagtctgca cccgcacaat atcgctcaga agaccgaggt catgatcgag cactttcgcc
     3661 atttcacgct gcacaagatc ggcggcaggg ctaaagccat ggtcgtcacc tccagccgcc
     3721 tgcacgcggt tcggtacaag caggcgttcg acaagtacat cacggagaag ggctacacag
     3781 ggatcaagac gctggtggca ttctccggaa cggtgattga cccggatgcg ccagacgtcg
     3841 agtacaccga gtcggggatg aacatcgacg cccaagacaa gcacatcggc cagaaggaac
     3901 ttccggagcg attcgccacc gaggaatacc aggtgctact ggtggcggag aaataccaga
     3961 cgggcttcga ccagccgctc ttgcacacga tgtacgtgga caagcgattg gccggcatcc
     4021 aggcggtgca gacgctgtct cgactgaacc ggacgcatcc gggcaaggag gacacgttcg
     4081 tgctcgactt cgtgaacgaa ccggacgaga ttctcgcggc cttccagccc tactacgaac
     4141 aaacgctcat tggtgagcag gccgacccga agcagctcta cgagcttcac gccaagcttg
     4201 acgcccagca ggtctaccac aaggcggaag tcgaggagtt ctgtaaaatc ttctacaagc
     4261 ccaagcgcaa tcagacggcc gctgaccacg cccggatgaa tgcgtgcatc gaccctgccg
     4321 tcggtcggtt caatgagctt gaagaggaag agcgcgagga attccgcaag acgcttacgg
     4381 cgtttcggaa tttgtactcg ttcctgtccc aggtgattcc gttccaggac accgacctgg
     4441 agaagctgta ttcctacatt cggttcctgc tgaccaagct gccgcgcgcc gactatgggg
     4501 ccgtgtacca cttcagcgac gacgtatcgc tcaagtacta ccggctacag aagatcggcg
     4561 aaggctcgat tgatcttgaa cccggcaaga cggagccagt cgacgggcca acggccgtgg
     4621 gtacgaaagc ggctcatccc gacgacattg agctgtcgag gctgatcgac atcctgaacg
     4681 agcgcttcgg cacggagttc aagccgggcg atgagctctt cttcgagtcg atccgcgagg
     4741 acgccgtggc cgacagcaac ctgcggcagg cagcgatggc gaacacgatg gagaactttg
     4801 gctacgtctt ccacaaggcg ctggaaggcc tgtttatcga ccgcatggac cagaacgagg
     4861 agatcacggc ccggtacatg aacgaggaac agttccgcaa ggcagtcggc caacacctgc
     4921 tcaaggacgt gtacgagcag gtccgaagcg aacaggcccg cccgccgacc cccgcccaag
     4981 agcccttccg gcgtgtcgaa tcaagggaag aggacaggta ccgcacctgc gtcccgctct
     5041 acacgctcaa ggcggccgcc ggtgagtttg gcgatgggca gcaggtcgaa cccgatggct
     5101 gggtggttcc gaaggtgccg cggcaactgt cagaaggcat gttcgtcgcg caggtcgtgg
     5161 gccgttcaat ggagccgcgg atccccgacg gcgcctggtg ccttttccgc tccccggtca
     5221 gcggaagccg tcaaggccgg atcgtcctgg tccagcaccg gtccattcac gacccggaaa
     5281 ccggcggcag ctacacggtg aaacgctacg agagcgagaa ggaagccgac ggcgaagggg
     5341 gctggcgtca ccaggtgatc cggctgcgtt ccgagaatcc cgagttcgat ccaatcacct
     5401 tgaagggtgt cacggaaggt gaggtatcgg tgattgcaga gctggttcag gtcttgagcg
     5461 ctacctaaga acggcatccg atcgacgtcc atcaaccctt cctatcagac atggtctcgg
     5521 ccgagctgcg tttgcccgga tgacagaagg ggcagaagat agcggaagaa gatagcggaa
     5581 agccaaccac agaggaggtt actcgctatg aacccgtttt gcacatttat ggcttctgcc
     5641 gccggtcgga tcatccgcat aattgtgggt gccgtattga tcgcttgggg cctattgtcg
     5701 ctcggaggca ccgtcggcat catcgtggcg gtggtcggtc tcgtaccgtt tctggccggc
     5761 ctgctggact tctgtgtatt cgctcccctc tttggctgcc cgtgggccgg cgcgcagatt
     5821 cgtgcaggta agtagcctgc ctattcctga tctaactcgg ctacatgttt cagctcgcag
     5881 agggagtgat tccagatcac gtcatgcacg tatccgggaa aggccaactt gaacaggtac
     5941 cagcccagtc tctttagggg gttccggctc gtcgggaagt tgaacgccac gtagatggtc
     6001 agaaatccgc cgcccggcct ggaccggtcg atatcgaagg tgagaacgcc gccccgcgga
     6061 aacccgccct gcacccgata cagcaggtaa cggccggcga ccaccttctc caggaccaca
     6121 ttgaaggaca gacgggacag caatacatcg taacggaccg tgcttcccac ctcgttcggc
     6181 ccacctacag tgcggtgcac gttgacgaat cgcgg
//
LOCUS       DASRKK010000566         8689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_243870, whole genome shotgun sequence.
ACCESSION   DASRKK010000566 DASRKK010000000
VERSION     DASRKK010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243870"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFC15_13230"
     CDS             <1..216
                     /locus_tag="VFC15_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11163.1"
                     /translation="MKQKLKTEAGGALYRMRKAIVEPVFGQIKEWRGFRRFLLRGLQK
                     VQAEWKLICLTHNLLKLFRSGRAWQRS"
     gene            complement(434..1006)
                     /locus_tag="VFC15_13235"
     CDS             complement(434..1006)
                     /locus_tag="VFC15_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM11164.1"
                     /translation="MPRTPDPELEGRIVDAAMRLLDRGGEGAITMRSVAREAGTTTPT
                     IYERFPDREALMRQVAERGTDELFSILQPVGRVENMCREYLRFSCAHPQRFDLTVEIF
                     GARFVAGEPRPVFDLFKSRLTEEIGVTGRKCEDLALAIAFLFAGTARGMVAAGGDTHH
                     ADELRRASLAALRLLLKAFSGPADPGRRTG"
     gene            1272..1916
                     /locus_tag="VFC15_13240"
     CDS             1272..1916
                     /locus_tag="VFC15_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11165.1"
                     /translation="MTKHRGPRQLCAWTLLAMLFATTLASATTSRREVNVVDLVANSE
                     LILRGTVANVTDGIDDRGIPYTEVTLHVSDAIRGQVSGDYTFRQFGLLKPRDMGNGRV
                     NLMVTPAGWATYTKGEEAILFLNKHAAWTGLQTTVGLGQGKFKVAMAGAVNQSNNAGL
                     FKNVTIDPTLLGTKEQRVMMTQQGPVNVNAFVGLVKQAINGQWIETGRMAHDQK"
     gene            1903..4254
                     /locus_tag="VFC15_13245"
     CDS             1903..4254
                     /locus_tag="VFC15_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11166.1"
                     /translation="MTRNKSGIFALLLTSCFALTSVAFAGGPLIVDPATKSAYHYDTS
                     QPIPVYYDLGNLGVVTDYSTNPPTLVTFDNTVGKNLVTKGFGDWSAIKTSSMRATVVG
                     DFSKIGLPDIDSTNVTDIIGKSNGRGIYVIFDADGSIMQNFFGVGPNVLGISSPQFSI
                     AGTTIITESWTVLNGTAIDPADTTAQYFQGVATHEFGHSLGMAHTQTNGAAFFYNDNV
                     GPNSCTSLPYANTLTSADVETMYPYINPYVGGTGMAQANLHTTDTLASFSDLYPGPGW
                     TNAYGTITGKVYDVDGKTELTGVNVIARNLANPYVDSTSALTGQMTQGLLGPDGSYTL
                     HGLKPGAQYVVYVDAIMAGGFPTPPLWFLPSAERFWVGIASNSTYNPCAYQPITASPA
                     KATTANITFARTPGAPLLYQLGYGSFVTGLSGDGVVAVGGYGRGGPIFRWTATQGVQP
                     MNVAGTGETVSISHNGRYMSSNLLDINSDTSLGAYRWDANNGWIRVRPNGACGTDTNS
                     NFAVSDDGSVFGLTYNTCTDYKAFRWIPNTAIGSIEYRSSFKRSDGNWANSRIDQVSA
                     DGSIAVGWQEAEWGGWLGTVWHNGRPELITDADGNPVSEAFTVSGDGSTLAGQLFDGQ
                     FPFDGSGWRRDTNSTTLQYVQPLNSDATPLTPYSLSGDGSVMVGLSGNPFLSLQFGPF
                     LWTKELGTVNLDDFVKKQGTAMDQWETLFAPGSISDGGATIAGTGIGFLGYAGWVMDM
                     HQVFVCHGTPGSKPQTISVAFPKAFDQHLAEGDIPGRCPDAHN"
     gene            complement(4375..4722)
                     /locus_tag="VFC15_13250"
     CDS             complement(4375..4722)
                     /locus_tag="VFC15_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11167.1"
                     /translation="MRKTLALLGIVVFSTALALAANKESGSTTLTNVQPAGTTDKQHK
                     KQQFDLTFSTSNNEYTCRTNENQKVQATQFVVGTTVNYKIDGNKGEVKSTSSGKNAKC
                     TLVRVAAITPAPQ"
     gene            5018..7771
                     /locus_tag="VFC15_13255"
     CDS             5018..7771
                     /locus_tag="VFC15_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZM11168.1"
                     /translation="MALKVKLAGSRPRVASKAPNRGSRRQPPPGHNFTLRDPLVKMLV
                     AGFLIAGILFMAGFAYFYVKYEGIVDRRMAGPLFNNAAKIYARPRTIEVGNPYTAQEI
                     ADYLRRAGYSEQNKDSDSPTGRFRFAGGSLEVMPGEESFHAPENVTIHFSGGKISAIA
                     AAGRGGEALTAYELEPQRITSLFEGQDRSKRQIVTFDEIPKNLVNAVIAIEDRRFFQH
                     SGVNYFRLMEAASTDILHGHRGQGGSTLTMQLSRGFFLSPEKTVKRKLTEMLIAIELE
                     QKFSKQRIFDMYANQVPMGQRGSFSINGFGEASRAYFNKDMKDLTLPECALLAGIIQR
                     PSYLSPYRHPERALERRNLVLDSMVETGSITRDQADHAKATPLKLAAPNVEASDAPYF
                     VDLVKDQLSSQYNESELNEQAMRIYSTLDPDLQRAAAEAVDAGMKLVDDQILKKRTHK
                     TKLGDGKTEVAVDSGPLPQVAVVVLDPHTGEVLALVGGRNYGTSQLDHAVAKRPTGSI
                     FKPFVYAAAVNTALTGQKLTFAAAQPADGENGPPKIDTSGASEIFTPATLVDDSQVSI
                     AYGDQVYEPRNYHEAFHGEVTARYALAMSLNNATVKVAQGVGFANVASLAKAAGITSV
                     RATPAIALGAYDASPLEMSGAYTVFGNGGTRVSPLMVKSVRDARGGVLNDYHNDSKGV
                     LDPRVAYVMTTMMQAVIDNGTGSTVRARGFTAPAAGKTGTSHDAWFAGYTSNLLCVVW
                     VGNDDYTDIKLAGGSTAAPIWAEFMKRAQKIPQYSNMKGFPAPSGVVQVQLDKVTNRL
                     ATPACPQTYYVAFIAGTEPKETCEQAFSDHRGLFSKILGLGSPEVAAPPPTTNGPVPG
                     QPGAPVAGQPSASQATGQPPPEQKKKKGFFSRVFGGKGNDQDPKENSADPPPNQGNKS
                     PPE"
     gene            7809..8609
                     /locus_tag="VFC15_13260"
     CDS             7809..8609
                     /locus_tag="VFC15_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11169.1"
                     /translation="MSPRQASRSFALCLACLIVIAAIASASPALDQQQPTAPVLVPLA
                     TGTPQELEKQGDQLRAQKRYLDSIDYYDAALAKQPSALLWNKKGIALLFLTRSKDAQK
                     CFQQGLKLDKNSAEALNNLGFVEQMQKNYGKAIKYYNKALVARPNSATFHYNLGAAYF
                     GKRDFSKATQEYQAAYQLDADIFQRVSKMGIMAQSSSPEDRAAFSFMVAKMYAQAGDF
                     EHSLEYLRKAMEQGYKGIGKVYTDSEFASLRTDPRFADLMAQKPPSLQ"
BASE COUNT         1853 a         2661 c         2483 g         1692 t
ORIGIN      
        1 atgaaacaga agctgaaaac ggaagcggga ggggctctgt accggatgcg caaggcgatt
       61 gtggagccgg tgttcgggca gatcaaagag tggcgtgggt tccgacggtt cctgctgcgc
      121 ggattgcaga aggtgcaagc ggaatggaaa ctgatctgtc taacccacaa tttgctcaag
      181 ctgttccgct ccggccgagc ctggcagagg tcttaaggaa gcaagaggaa aagccacgca
      241 taggccgcaa aaatggcctt gccggcttac gacggcaaga aatgaggtaa cgccggtttc
      301 agtttgcgat tttgccgatg cgcgatggac ctcgaacggc aaggtcaagg tcaaggtcaa
      361 ggttgtgatt ccgtattccc gacaaactcc tagcctcggc tcgacccccg gtgtctgcca
      421 tcttccacgc cgatcagccg gtccgtctcc ctggatcggc aggaccagaa aaagctttca
      481 gcagtagccg caacgccgcc agcgaagcgc gacgcaattc gtcggcatga tgagtgtccc
      541 cgccggcggc gaccattccc cttgccgtgc cggcaaacag aaaggcgatg gccagcgcga
      601 gatcttcgca tttccgcccg gttactccga tttcctcagt aaggcgggac ttgaaaagat
      661 cgaatactgg tctgggttcg ccggcgacaa accgtgcgcc gaatatctcg acggtcaaat
      721 cgaagcgttg gggatgggcg cagctgaaac gaagatactc gcggcacatg ttttccacgc
      781 gaccgacagg ctgcaaaata gaaaacaatt cgtcggtgcc acgttcggca acctggcgca
      841 taagggcctc ccggtcggga aagcgctcat aaattgtggg cgtggtggtc cccgcctccc
      901 gggcgaccga gcgcatggtg atggcgcctt cgccaccgcg gtccagcagg cgcatggctg
      961 cgtctacaat ccggccttcc aactcaggat caggtgttct tggcatgccc tcggccccgg
     1021 cgtgaaagaa ccagacaatt ttagcattga catcgtttgt tttacaatgt ataaataaca
     1081 gtgtacaagt aacagtcgat tccatcaagg ttagggattg cacggtccca atgcgctgta
     1141 cccggtacca cttaccgggc atcgcaccag cgatacacgg gtccagattc gcaacctgct
     1201 cgacactcgt accgaagccg agcgccagtc cgtctgcgag acaaacaagt ttgctacaag
     1261 gagcaccacc gatgacaaaa catagaggtc cgcgccaact gtgcgcctgg acccttttag
     1321 ccatgctgtt cgcgaccacc ctcgcgagcg ccaccacatc ccgccgcgag gtcaacgtgg
     1381 ttgacctggt tgccaactcc gaactgatcc ttcgcggcac cgttgccaac gtgaccgatg
     1441 gaatcgacga tcgcggaatt ccctacaccg aggtgaccct gcatgtttcc gacgccatcc
     1501 gcggccaggt cagcggagat tacacctttc gccagttcgg gttgctgaag ccccgtgaca
     1561 tggggaacgg aagggtcaac ctgatggtca cgcccgccgg ttgggccacc tacaccaagg
     1621 gcgaggaagc catcctcttc ctgaacaaac acgccgcctg gacgggcctg caaactaccg
     1681 tggggctggg acaaggcaag ttcaaggtcg cgatggcagg cgcagtcaac cagtcgaaca
     1741 atgctggtct tttcaagaat gtcaccattg accccacctt gctggggacg aaagagcaac
     1801 gggtcatgat gacccagcaa ggcccggtca acgtcaacgc attcgtcggc cttgtgaagc
     1861 aggcgatcaa tggtcaatgg atcgagaccg gaaggatggc gcatgaccag aaataagtcc
     1921 ggtatcttcg cactcttgct cacctcttgt ttcgcgctaa catcagttgc gtttgccggt
     1981 ggaccactca tcgtggaccc cgcaaccaag tctgcttacc actacgacac cagccaaccc
     2041 attcccgttt attacgactt gggcaatctt ggcgtcgtca cggactactc tacgaatcct
     2101 ccgacgctgg tcaccttcga caacaccgtc ggcaagaacc ttgtcaccaa gggcttcggc
     2161 gattggtccg ccatcaagac ctcgtcaatg cgggccaccg tggtcggcga tttctccaag
     2221 attggcctgc ctgacatcga ctcgacgaat gtcactgaca tcatcggtaa gtcgaatggc
     2281 cgcggtatct atgtgatctt cgatgctgac ggcagcatca tgcagaactt cttcggcgtc
     2341 ggacccaacg tgctgggcat ttccagcccg cagttcagca tcgccggcac cacgatcatc
     2401 actgaaagct ggacagtgct caacggtacg gcgatcgacc ccgccgacac taccgctcag
     2461 tactttcagg gcgtcgccac gcacgagttc ggtcattctc tcggcatggc gcacacccag
     2521 accaacggcg cagcattctt ctacaacgac aatgtcggac cgaatagctg caccagtctg
     2581 ccctacgcca acactctgac cagcgccgat gtggagacga tgtatcccta catcaatccc
     2641 tacgttggcg gcacaggaat ggcacaggcg aaccttcaca ccacggatac cctcgcttct
     2701 ttctccgacc tgtaccctgg tcccgggtgg acgaatgcct acggcaccat caccggcaag
     2761 gtgtacgacg ttgacggtaa gaccgagctg acgggtgtca acgtgattgc ccgcaatctc
     2821 gccaacccct acgttgactc gacttccgca ttgaccggac agatgacgca aggactgctc
     2881 ggaccagacg gcagctacac gctgcacggc ctaaagccgg gcgcgcagta tgtggtgtac
     2941 gtggatgcca tcatggccgg tggattcccc acccctccgc tgtggttcct gccctcggcc
     3001 gaacgcttct gggttggcat cgcgtcgaac tcaacctata atccgtgcgc ttaccagccg
     3061 atcaccgcca gcccggcaaa agcaaccaca gccaatatca cctttgctcg cacgccaggt
     3121 gctcccttgc tctatcagtt gggttatggg tcgttcgtca ctgggctcag tggagatggc
     3181 gtcgtcgccg tgggtggcta cggccgcggg ggaccgatct tccgctggac cgcaacacag
     3241 ggcgtgcagc cgatgaacgt agccggcacc ggcgaaacgg tgtcgatctc gcacaatggt
     3301 cgctatatgt ccagcaatct gttggatatt aattccgaca ctagcctcgg cgcctatcgc
     3361 tgggatgcga acaacggctg gatcagggtc aggccgaacg gagcatgcgg gaccgatacc
     3421 aactcgaatt tcgcagtcag cgacgacggg tcggtattcg gcttaaccta taacacctgc
     3481 accgactaca aggcgttccg ctggattccc aacacggcca tcggctcgat cgagtacagg
     3541 tcgtcattca aacgctccga cggaaattgg gccaacagcc gcatcgacca ggtatcggct
     3601 gacggttcaa ttgctgtggg atggcaggaa gccgaatggg gtggctggtt gggcaccgtt
     3661 tggcacaacg gccgacctga gttgatcacc gatgcggatg gcaatccggt aagcgaagcg
     3721 ttcaccgtca gtggtgacgg atccactctc gcaggtcaac tcttcgacgg tcagtttccc
     3781 tttgacggca gcggctggcg cagagacacc aactcgacaa ccttgcaata cgttcagccc
     3841 ctcaactccg atgccactcc gctcaccccc tattcgcttt cgggggacgg cagcgtcatg
     3901 gtgggcttga gtggcaatcc attcctgagt ctccagttcg ggccgttcct gtggacgaag
     3961 gagttgggta cggtcaacct ggatgacttc gtaaagaagc agggcacggc gatggatcag
     4021 tgggaaaccc tgtttgctcc cggctccatc tcggatggtg gcgcaaccat cgcaggtacg
     4081 ggaattggct tcctgggcta cgccggctgg gtgatggata tgcaccaggt gtttgtctgc
     4141 cacggcactc ccggcagcaa gccccagacc atcagcgttg cgttccccaa ggctttcgat
     4201 cagcacttgg ccgaaggaga catccccgga cgttgtccgg acgctcacaa ctaactcggg
     4261 ttaaagtttc ccccgaacgg cggtggcgcg attccccaac gtgctgccgc cgttttcgtt
     4321 gccctcgaca tcagagtgag gtccggaacg ctggcggcgg gtggcgagag ccgattactg
     4381 cggggccggt gtaatcgctg ccactcgcac cagcgtgcac ttcgcgtttt tcccgctgga
     4441 cgtgctcttc acttcgcctt tattgccgtc gatcttgtaa ttcacagtcg ttcccacgac
     4501 gaactgcgtc gcctgcactt tctggttttc gttggtgcgg caggtgtatt cgttgttgga
     4561 agtcgaaaac gtaaggtcga actgctgctt cttgtgctgc ttgtcagtcg ttcccgccgg
     4621 ctgaacgttg gtcagggtcg tgcttcccga ttccttgttg gctgccaagg ccagagcagt
     4681 cgagaaaacg acgatcccta acagcgccag tgtcttccgc atagcgtgca ctcctcgaaa
     4741 aaatccactg gcattgacta taccaacgag gtacctgcgg acggaaaacc aagccggagg
     4801 agcactacgg acgttgctgt tttacaataa cggtattgct ctggcggcag cctttgccgg
     4861 atattcgtgg cctgaggtgt tgtgcgcgct ggagagctgt gcctggccat gccggtggct
     4921 cggagcagca cagtttccca ggagttcggc atctcagata acagaatgcc cttgtgggcc
     4981 gtcttactat ttacggcttc tctgcgtacc gataattgtg gcactcaaag ttaaattggc
     5041 tggaagccgg cctcgcgtag cctcgaaagc gccgaaccgt ggcagccgtc gccagccccc
     5101 gccgggacat aatttcaccc tgcgcgaccc cctcgtaaaa atgctggtag ccgggttcct
     5161 gatcgccgga atcctgttca tggcgggctt cgcctacttc tacgtcaagt acgaaggaat
     5221 tgtggaccgc cgcatggcag ggccgctctt caacaacgct gcgaagattt atgcgcggcc
     5281 gcgaaccatc gaagttggca atccctacac ggcgcaggaa attgccgact atttgcggcg
     5341 tgccggttac agcgagcaaa acaaggacag cgactcccct accggacgtt ttcggtttgc
     5401 aggcggcagc cttgaggtca tgccgggaga ggaatcgttc cacgcgcctg agaatgtgac
     5461 catccacttt tcgggcggca agatcagtgc gatcgccgcc gcaggccgtg gaggcgaggc
     5521 ccttacggct tatgaactcg agccgcagcg gattacctcg ctgttcgaag ggcaggaccg
     5581 gtccaagcgc cagatcgtca cctttgacga gattcccaaa aacctggtga acgccgtgat
     5641 tgccattgaa gaccggcgct tcttccagca cagcggggtg aattattttc gcctgatgga
     5701 ggccgccagc accgatattc tgcacggcca tcgcggacaa ggtggatcga ccctgaccat
     5761 gcagctttcg cgcgggttct tcctttcgcc cgagaaaacc gtcaagcgca agctgacgga
     5821 gatgctgatc gccatcgagt tggagcaaaa attctccaag caacgcatct tcgacatgta
     5881 cgccaatcag gtgccaatgg gccagcgcgg ctcgttcagc atcaacggtt tcggtgaagc
     5941 ctcgcgcgca tactttaaca aggacatgaa ggacctcacg ctgcccgagt gtgccttgct
     6001 cgctgggatt attcagcgtc ccagctacct ttcgccctac cgccatccgg agcgcgcgct
     6061 ggagcgccgc aacctggtgc tcgactccat ggtggagacg ggctcgatta ctcgcgatca
     6121 ggccgaccac gccaaggcca ctcccctgaa gctggcggcc ccgaacgtgg aagccagcga
     6181 cgcgccctac tttgttgacc tggtaaagga ccagctctcc agccagtaca acgagagtga
     6241 actaaatgag caggccatgc gcatctactc cacgctcgat cctgacttgc agcgcgcggc
     6301 cgccgaggca gtggacgcag gcatgaagct ggtcgacgac cagattttga agaaacgcac
     6361 ccacaaaacc aaacttggtg acgggaagac ggaagtcgcc gtcgactccg gaccgctgcc
     6421 gcaggtcgct gtagtggtcc tcgatcctca cacaggggag gttttggcgc tagttggcgg
     6481 ccgcaattac gggacgagcc agctcgatca tgccgtcgcg aagcgtccca cgggatcgat
     6541 cttcaagccc ttcgtgtacg ctgcggcggt caacacagcc ctgactgggc agaagctgac
     6601 attcgcggcc gctcaacctg ccgacggcga aaacggtccg cccaagattg acaccagcgg
     6661 cgcatcggaa attttcacgc cagcgaccct ggtggacgat tcgcaggtga gcatcgccta
     6721 cggcgaccag gtgtatgaac cgcgcaatta ccacgaggcg tttcacggcg aagtcaccgc
     6781 ccgctatgcg ctggcgatgt cgctcaacaa cgctacggtg aaggtggcac agggcgtagg
     6841 gtttgccaat gtggcctcgc tagccaaggc ggcgggcatc accagcgtgc gggccacgcc
     6901 tgctatcgct ctgggcgcct acgatgccag ccccctggaa atgtcgggag cctacaccgt
     6961 cttcggcaat ggcggcacgc gtgtctcccc cctgatggtg aaatcggtgc gcgatgctcg
     7021 aggcggcgtt ctgaacgatt accacaacga ttccaagggt gtgctcgacc cgcgtgtggc
     7081 ctacgtgatg acgaccatga tgcaagcggt catcgacaat ggtacgggct cgactgttcg
     7141 cgcccgaggc ttcaccgctc ccgccgccgg caagaccggc acatcccatg acgcgtggtt
     7201 cgcaggctac accagcaacc tgctgtgcgt ggtgtgggtg ggcaacgacg actacaccga
     7261 catcaagctg gctggaggat cgacggcagc gcccatctgg gcggagttca tgaagcgggc
     7321 gcagaagatt ccgcaatact cgaacatgaa aggttttccc gcgccgtccg gagtggtcca
     7381 ggtccagttg gacaaggtca ccaaccgcct ggcaaccccg gcgtgcccgc aaacctacta
     7441 cgtggcgttc atcgccggga ccgaacccaa ggaaacctgc gagcaggctt ttagcgacca
     7501 tcgcgggctc ttctccaaga tccttggact aggctccccc gaggttgccg cgccgcctcc
     7561 aaccaccaac gggcccgtgc caggtcagcc gggggccccg gtcgccggac agccaagcgc
     7621 ctctcaagcc actggacaac cacctccgga gcagaaaaag aagaagggct tcttcagccg
     7681 ggtctttggg ggcaagggaa acgatcagga cccaaaagaa aatagtgcgg atcccccgcc
     7741 caatcaaggg aacaaatccc cgcctgaata ggtctaatat agtgggaacg ggagtcgggg
     7801 gtaggggtat gtctcctcgc caagcgtcac gcagtttcgc gttgtgcctt gcgtgtctta
     7861 ttgtcatagc cgcaatagct agcgcctcgc ccgcattgga ccagcagcag ccgacagcgc
     7921 ctgtgctcgt gccgctcgcg accggcacgc ctcaggaact ggagaagcag ggagaccagc
     7981 tacgcgccca gaagcgctac ctcgactcca ttgattatta tgacgcggct ctcgccaaac
     8041 aaccttcagc cctgctctgg aacaagaagg gcattgctct gctcttcctc acccgcagca
     8101 aggacgcgca gaaatgcttt cagcagggcc tcaagctcga caagaactcg gctgaggccc
     8161 tgaacaacct gggcttcgtc gaacagatgc agaagaacta cggcaaagcc atcaagtatt
     8221 acaacaaggc gctggtggcc cgtcccaatt ccgcgacctt ccattacaac ctcggcgcgg
     8281 cctacttcgg taagcgcgac ttcagcaagg caacccagga gtaccaggct gcctaccagc
     8341 tcgatgccga catattccag cgcgtctcga aaatggggat catggcgcag tccagctctc
     8401 ccgaagatcg cgcggcattc tccttcatgg tggccaaaat gtacgcccag gccggcgact
     8461 tcgagcattc gctggagtat ctgcgcaaag cgatggaaca aggctataaa gggatcggca
     8521 aagtctacac tgactccgag ttcgccagcc tgcgcaccga tccccgcttc gccgacctca
     8581 tggcacaaaa gcccccgtcg ctccagtaga gctgggggat tggtggtagc tgggtgtccc
     8641 actcaagccg ttgttgcttg agtgggtctt ttgactttgc tggcaactg
//
LOCUS       DASRKK010000567         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_49838, whole genome shotgun sequence.
ACCESSION   DASRKK010000567 DASRKK010000000
VERSION     DASRKK010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49838"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1046
                     /locus_tag="VFC15_13265"
                     /pseudo
     CDS             202..1046
                     /locus_tag="VFC15_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1332..2408
                     /locus_tag="VFC15_13270"
     CDS             1332..2408
                     /locus_tag="VFC15_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM11170.1"
                     /translation="MAVTGGTRRIVGGVDTHKDLHVAAVVDEQDRVIGTRSFATTRQG
                     YRQMLAWMRSFGELQRIGIESTGSYGAGLLRFMQQAKVAVLEVTTPDKQDRRRRGKND
                     DLDAQNAAHAAFAGQRTVTPRSRDGMIESLRILMACRKTAVSARRVALQMIHNTIVCA
                     PDGLRDQLRSLTRMQLVRTLASWRPDLTAYRDLDSAYRIGLKSLARRYLELHDEIADL
                     DVMIAAIVDELAANLVARNSIGHIGAAQLLLTAGDNPERLRSEASFASLCGVSPVPAS
                     SGKTVRHRLNRGGDRAANSALHIIAIGRLRTDQRTKDYVARRIAEGHSKLDAIRALKR
                     YLAREVFTLITQRRKEINATRIAA"
     gene            2425..2655
                     /locus_tag="VFC15_13275"
                     /pseudo
     CDS             2425..2655
                     /locus_tag="VFC15_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          578 a          845 c          850 g          517 t
ORIGIN      
        1 cgccctagta cgcctttacc ggtagtcgcc agggtgaagt tcgcattaga ctcgtatcgc
       61 gttcaggtta tagcgaacac acgattacgt atgatggcgt cggtctgccg gcagagagag
      121 ctggctcggg aaatctgaac aattcggata agtggaaatt ctgcctgaac gcggcgtagg
      181 ctgccgtgaa caggagagag catgacgaag agaccacgcc ggaaccacag cccggccttc
      241 aaggcgaagg tggctttggc ggctatcaag ggtgagaaga cgctggccga actggccgaa
      301 caattcgatg tccatccgaa ccagatcacg acctggagat cgcagcttct cgaaggggct
      361 gccggggttt ttggcgccga gacgaaaccc gattcaagtc cgcccgcgat cgacatgaaa
      421 acgctgcacg ccaagatcgg ggagttgaca ctggaacgag attttttgtc cggagcgctc
      481 gggaaagccg gacttttgcc gagcgcaaag cgatgatcga ccggaacgat gacttgccgg
      541 tgacccgaca ggcgaaggtt ctcgggatca gccggggcag cgtgtattac ttgccccgga
      601 cggtctctgc cggcgatctg gcgctgatgc gccggattga cgaattgcat ctcgattatc
      661 cgttcgcggg cagccgcatg cttcagactt ttctggtccg cgagggatac gatgccgggc
      721 gcctccacgt tgccaccctg atgaggcgga tgaggatcga ggcgatctat cgccggccca
      781 acacgtccaa acctgcgccg gggcacatga tcttcccgta tctcctgcgc aagctgccgg
      841 tgacgcggcc caatcaggtc tgggcgatgg atatcacgta tatcccaatg gcgcggggct
      901 tcgtctaact tgcggccgtc gtcgactggt tcagccggag ggttctggca tggaagttat
      961 cgattacgtt ggaaacggag ttttgtctcg aggcggtcga ggaggctctt gcccgatatg
     1021 gcaagcccga gatcttcaac actgatagtc agaggataaa cgccagtttc cgggcggcct
     1081 gactcaaaca ccgatcggcc gactggtagg ctggcgccgc aaggcgcccg gtgcggtctg
     1141 acccgcactc cgatcgacca gccgtgtctt tccctggacc tgtcgaccgc ccgggaacgc
     1201 cttgcggtga ggctttgcct cgccgatgcc tgtgacccga gaagggcctg acctccatgg
     1261 tctcgtgact gtcctgtccg cgcattcggt cccggcgaag ccattcatgc aggggtatgg
     1321 ggaggctggg aatggcggta accggaggca cacgtcgcat cgtcggcggc gtcgataccc
     1381 acaaggatct tcacgtcgca gcggtcgtcg acgaacagga ccgggtcatc ggaacacgat
     1441 ccttcgccac gactcggcaa ggctatcgcc agatgctcgc ctggatgcgg tccttcggag
     1501 aactacaacg tatcggaatc gagtcgaccg gcagttacgg cgcagggctc ctgcgcttca
     1561 tgcagcaggc aaaggtcgcg gtcctcgagg tcacgacccc cgacaagcag gatcggcgtc
     1621 gacgcggcaa gaacgacgat ctcgacgccc agaacgccgc ccatgctgcc tttgcaggtc
     1681 agcggaccgt gacgccgcga agccgggacg gcatgatcga atccctacgg attctcatgg
     1741 cttgccggaa gacggcggtg agcgccaggc gcgtagccct gcagatgatc cacaatacca
     1801 tcgtctgcgc ccctgatggc ctgcgcgatc aactgcgcag cctgacccgc atgcagctcg
     1861 tccgcacgct ggcctcgtgg cgacccgatc ttaccgctta tcgcgacttg gattccgcct
     1921 atcgcatcgg cctgaagtcg ctggcccgcc gttacctcga actgcatgat gagatcgcag
     1981 atctcgatgt gatgatcgcc gcaatcgtcg acgagctggc ggcgaacctc gtcgcccgca
     2041 attcgattgg ccacattgga gctgcgcagc ttctccttac ggccggcgac aatccagaaa
     2101 ggttgaggtc agaggccagc ttcgcatcgc tgtgcggcgt cagtccggtt ccggcgtcgt
     2161 ctggaaagac cgtcaggcat cggctcaacc gcggcggaga tcgcgccgcc aatagcgcgc
     2221 tacacatcat cgccatcgga cgcttacgga ccgaccagag aaccaaggat tacgttgccc
     2281 gtcggatagc agaagggcat tccaaactcg acgccattcg agccctgaaa cgctaccttg
     2341 cgagagaggt cttcacgctc atcacacagc gtcgcaagga aatcaacgcc acgcgaattg
     2401 ccgcttgaca aacagaaggg cgtccagggc tcccagttca ccagcatcgc cttcaccggc
     2461 ctgctgctca agaacgagat caagatcagc atggatggga aaggcgcctg gcgagacaac
     2521 atcttcgtcg agcgcctgtg gcggaccatc aaatacgagg aggtgtacct gcgggcctac
     2581 gccagtgtcc cggaggcacg ggcctccata ggccgatata tcgacgcctt ttacaatgcc
     2641 agaaggcctc attcgagatc ccatgggggt gtggatagcg accagctctg ggggcagact
     2701 gaggttgctg atccgctcac tctggaggcg acccatgtca ttcgatcacc attccgaaga
     2761 accgaccgct atccgaacca atcttgcggc
//
LOCUS       DASRKK010000568         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_49933, whole genome shotgun sequence.
ACCESSION   DASRKK010000568 DASRKK010000000
VERSION     DASRKK010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49933"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1988
                     /locus_tag="VFC15_13280"
     CDS             342..1988
                     /locus_tag="VFC15_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM11171.1"
                     /translation="MFVRTQTNGSRTYLLIVDNQWVEGKVRQRVLLRLGRLDELLANG
                     RLDSLIQSLGRFSEKLAVLGAHARGDSITTRSARIGPALIFQRLWQLCSIDKVLTALL
                     EGRRFEFSVERAIFLTVLHRLFAPGSDRAAEKWKHDYAIDGVADLNLHQLYRAMAWLG
                     EVLPKSQQDGATPFAPRTNKDLIEEQLFARRRDLFSDLDIVFFDTTSIYFEGEGGETI
                     GQRGHSKDHRPDLKQMVVGMVLDQNGNPVCSELWPGNTADVKSLVPIVERLRSRFGIG
                     SVCIVADRGMISAETLAEVERRKWQYILGVRMRSSTEAKAVVARAGRYAEVHPKSDDP
                     NDPSPLKVKEVWVEDARRYVVCVNEDQATKDGHDREAVVASLRDALSKGDKSLVGNKG
                     YRKYLRAGGKQFTVDEDKIQEEARYDGKWVLTTNTDLPAREVALKYKQLWMVEDVFRS
                     MKSLLDTRPIFHKCDETIRGHVFCSFLALQLRKELEDRLARKEWKLEWADVIRDLDNL
                     LEMEVAISGKGYVFRGQSPGVAGKVFQACGVALPPALRSC"
BASE COUNT          452 a          622 c          687 g          415 t
ORIGIN      
        1 gtgtagccgc cctcccaggc gcctttcatc gtaaggcagc ctggcgctgg gatgagttga
       61 ttgtgtggac gataggcgga gtcctgcgcc agaccaagcc gcggcgcata caatgcgaca
      121 gcggccactg ccagcacagc ggctctacga gacaagatcg accaggatct caagctcatc
      181 ttctacacct ggcgacgcca agtggctgca actcactgat tccaaacatg gcggtatcca
      241 caggcacaaa atcctagtgt cacgcatgat ttgccaatgg cggataattc gcttgtaatg
      301 cgccttgtag tcagaaatga tagtgacaca ggagcgctga tttgttcgtt cgcacgcaga
      361 ctaacggttc ccgaacttat ctgctcatcg tggataacca gtgggtggag ggcaaggtca
      421 ggcagcgcgt tttgctgcgt ctcggccgcc tggatgagtt actcgccaac ggccggctcg
      481 actcgctgat ccagtcgctg ggacggttct cagaaaaact cgccgtcctc ggtgcccatg
      541 cgcggggcga cagcatcacc actcgcagcg ccaggatcgg gccggctctg atcttccaac
      601 gactctggca gctctgctcg atcgacaagg tcctgacggc cttgctcgaa ggccggcgct
      661 tcgaattttc cgtcgagcgg gccatcttcc tcaccgtgct gcatcgcctc ttcgcgcctg
      721 gcagcgaccg tgccgccgag aagtggaagc acgactacgc catcgacggc gtcgccgatc
      781 tgaacttgca ccagctctac cgggccatgg cctggctggg agaggttctt ccgaaatccc
      841 agcaggacgg ggccacgcct ttcgctcccc gcaccaacaa ggacctcatc gaggagcagt
      901 tgtttgcgcg gcgccgtgac ctgttctccg atttggacat cgtatttttc gacaccacgt
      961 cgatctactt cgagggcgaa ggaggcgaga ccattggcca acgcgggcac tcgaaagatc
     1021 atcgtcccga tctgaagcag atggtggtgg gcatggtgct ggaccagaac ggcaatccgg
     1081 tctgcagcga actctggccg ggcaacacgg ccgacgtgaa gagcctggta cccatcgtgg
     1141 aacggttgcg gagccgcttc ggaatcggct cggtctgcat cgttgcagac cgggggatga
     1201 tcagcgccga gaccctggcg gaagtggaac ggcggaagtg gcaatacatt ctgggcgtgc
     1261 gcatgcggag ttcgacggaa gccaaggcgg tggtggcgcg cgccggccgc tatgccgaag
     1321 tgcatcccaa gagcgacgac ccgaacgatc cctcgccgct gaaggtaaaa gaagtctggg
     1381 tcgaggatgc gcgccgctat gtggtgtgcg tgaacgagga tcaagccacc aaggacggcc
     1441 acgaccgcga agccgtggtg gcctcgctgc gcgacgcgtt gagcaagggc gacaagtcgc
     1501 tggtgggtaa caaaggttat cgcaagtacc tgcgcgcggg aggcaaacag ttcaccgtgg
     1561 acgaggacaa aatccaggag gaagcgcgct acgacggcaa gtgggtgctg acgaccaaca
     1621 cggatctccc ggcgcgtgag gtggcgctga agtacaagca gttgtggatg gtcgaggatg
     1681 tcttccgctc gatgaagtcg ctactggata cccggccgat ctttcacaag tgcgacgaaa
     1741 cgattcgtgg gcacgtgttt tgctcgtttc tcgcgctaca gttgcgcaag gaactggagg
     1801 atcgtttggc gcgcaaggag tggaagctgg aatgggccga cgtgatccgg gatctggaca
     1861 acctgcttga gatggaagtc gccatcagcg gcaagggtta cgtttttcgc ggccagagtc
     1921 ccggggtggc cggcaaggta tttcaagcct gcggcgtcgc cctgccaccc gcgctgcgct
     1981 catgctgaac ttatcctcag ttcggcagtt cggccgaggt ggagaaaaag cgtgtcacta
     2041 gggcgttttc gaaattacaa cctattggaa atggacaact tccgtcggca tggtgtagaa
     2101 gatgagtcag acaagccgcc gtcccaactc gggcagccga acgctttatc ttctcgacgc
     2161 ctgcacgcga tagtcg
//
LOCUS       DASRKK010000569         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_146623, whole genome shotgun sequence.
ACCESSION   DASRKK010000569 DASRKK010000000
VERSION     DASRKK010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146623"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1469..>2453
                     /locus_tag="VFC15_13285"
     CDS             1469..>2453
                     /locus_tag="VFC15_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZM11172.1"
                     /translation="MASAQPLSSGEPQPSLPQPFPSALRTGHVAQRLFWLVPMHSWFD
                     APVRLAKDRFNSENEARKTLATMLGAFMVLGGAYFTWRNIKLVQEGQITDRFTKAIEQ
                     LGAVDASGKKKLEVRLSGIYALERIANPSHSRSRLTSTSRETCKGRPIDLSLSAKPCL
                     RQLSQLRNCPSPRTNRQRTWCAFREAMLAFGGDGRAVGMADNKTKPTKLSVAAVIDAL
                     TDPTRRADAKALVKLMQRATGEKPKMWGPSIIGFGSYHYTYDSGREGDMPLICFSPRK
                     AATVLYNMTGFSHSEALLAKLGKHTTGKGCLYIKTLSDVDQQVLEALVVKSV"
BASE COUNT          606 a          689 c          659 g          499 t
ORIGIN      
        1 ttgtgcggac tcaacaactt agcttcacga tatgtcgtgc tgttcgggct gaattgcgca
       61 actcggcacg cgactatgca accaaatatg caaccacacg ccggaggtgc tcgcaatccc
      121 tccggccaca ttttccctat cccatcccac tctgagccgg acagtcttct cagcaacgag
      181 acacccggca ccctacagct taacggtgcc gcggctaatg gatggcagat gcggccacca
      241 ccaaggctgg caaaagctgc cctcaccgag ctgttgagag cggatcaaag tcctttcgct
      301 ccgatcccga acactccagg cagcggccgt acatgattga gcaggagcat ccgcgttcgg
      361 aatagaattt gctgctcgga gattgcacaa tgtctcacgc tcgaaaggta cagcgggggc
      421 agcatgaaaa tcgaccttca cggataccac ccgtctgaca tcgtctggaa cggcgttctt
      481 gcaaaaattg tgcagcaagc gtgggagatg ggtgagcggc gactctgttt aatacacggt
      541 cacggacgca atcgcggaat tacgccggga tttgtgaata cgaattcgca gtatcaagta
      601 tcttcaggcc gcagccccta tcggaaactg gcatcactga agagtattta agagacgaac
      661 aatcccgcag ggggtatcag gggtttggct cggatgtgct gcatccctct ggcagcccac
      721 tgcgggcagg agctaaccat gttaatgtta agtatcacac aatcgcaggt cgttgtaagt
      781 gatggccgtc acgatgcggc gctcgtaatc gatcgagaga ccacacacaa ggccggaatt
      841 ggcgtcaaca caccttgagg acactcccga agaagacgcc aagcctttgt acaggcgcac
      901 atctgccaaa tcgtggtcaa ggacggccca ccccggcgtc aggccagtct cataggatac
      961 actcggaaca cgtctaatta cggcatttct ggaggcggat ttggcggaag ttcggtcaca
     1021 agaaggcgag tcaatagaaa accttttacg tcgcttcaag cgaaaagttc aaacggaaga
     1081 cattatcaag gatgtgaagc gtcactcctt ttacctgaag ccgggcgaga aaaaacgtgt
     1141 gaagcaagct ctggcacaga aacgtgcgcg gaaaaagacc cgtacaaata cggacgaaag
     1201 gcgataatcg ggctgccgtt tctaaggtgc aatcgcttca ctgacgcaag ccgggtcgcc
     1261 aacaacaatg cgccgactcc gcccaccgca atttcccagt caacgatctt tgtgacaact
     1321 ggtcaggcga gagtattttt aacgtcagcc ctctgcgctg aattttgcaa tccattgcaa
     1381 aaaacttgcg aattctacgt ccctttcttt ctggccgccc accgtgcctt catcatggcc
     1441 gacaatttcc tccgcatggc tgcactcatt ggcttccgcc cagccgcttt cttctggcga
     1501 gcctcagcct tctttgccgc agcctttccc ttctgctttg cgcaccgggc acgttgcgca
     1561 gcggctattt tggctcgtgc cgatgcactc atggttcgac gcgcccgtcc gcctggccaa
     1621 ggatcggttt aacagtgaaa acgaggctcg taagacgctc gcgacaatgc tgggcgcgtt
     1681 catggtgctg ggaggcgctt actttacttg gcggaacatc aaattggtgc aagaaggaca
     1741 aatcacggac aggtttacca aagccatcga gcagcttgga gcggttgacg ccagcggaaa
     1801 gaaaaagctg gaggtccgat taagcggaat ctacgcgctg gagcgcattg ctaacccgag
     1861 ccactcccga tcaaggctaa cgagcacgag ccgggaaact tgtaagggaa gaccgataga
     1921 tctctcactg agcgcaaagc cctgtttacg ccaactgagt caacttcgga attgtccgtc
     1981 acccaggact aaccgccagc ggacctggtg cgcttttcga gaagccatgt tagccttcgg
     2041 aggagacgga agggcggtag gaatggcgga caacaaaacc aagcccacca aattaagtgt
     2101 agcggcggtt atcgatgccc tcacagatcc gaccagacgg gcagacgcca aggcgctcgt
     2161 caagttgatg caaagggcga ccggcgaaaa gccgaagatg tgggggccat caatcatagg
     2221 cttcggcagc taccactaca cgtacgacag cgggcgcgaa ggcgatatgc ctcttatctg
     2281 tttttctccg cgtaaggccg caactgttct gtacaacatg accggtttca gccactctga
     2341 ggcgctgctc gcaaagctcg gcaagcatac gaccggcaag ggctgcctct atatcaagac
     2401 gctttctgat gtcgaccaac aagtgttgga ggccctggtg gtcaaatccg tcg
//
LOCUS       DASRKK010000570         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_244216, whole genome shotgun sequence.
ACCESSION   DASRKK010000570 DASRKK010000000
VERSION     DASRKK010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244216"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            563..847
                     /locus_tag="VFC15_13290"
     CDS             563..847
                     /locus_tag="VFC15_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11173.1"
                     /translation="MAERRTKIPFPTPTSPLMDGSEVGVAESTEKWSEVTLEDGTVIR
                     IKPSVISAIRMDGQYDPEGNPVYAVKANHIVTITSTPPHLRRGSKDAKIN"
     gene            852..1343
                     /locus_tag="VFC15_13295"
     CDS             852..1343
                     /locus_tag="VFC15_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11174.1"
                     /translation="MTRTQSITADKTDIIMLTAYVLLYPGIAGATSQQQQETICSNTS
                     EPSIDASLTSPPDCVVDPTTKRWLRFQNLVDEWRHERGAMSSITEMATLRPYQNIIGM
                     GEEAIPLILAELKSEGDEPDQWFWALGAITGANPVSPEDQGNFAKMAQAWFKWAEDEG
                     HAG"
     gene            2022..2378
                     /locus_tag="VFC15_13300"
     CDS             2022..2378
                     /locus_tag="VFC15_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11175.1"
                     /translation="MYDDFQAVDRWTGKPVHCRYQALIVAIATRHADAVDIKFLLEDR
                     PVWIALPHPAWVEYRRQTGKVITDPMAVQIAGHYLKWAIESGEDNGREMCSLNVQETL
                     DHLQAVVDEAHQLVTR"
BASE COUNT          571 a          644 c          690 g          494 t
ORIGIN      
        1 ccgcaggacc tcgcgaacgc gatgaataaa cgtcgcgcgg gcgacaccgt caccgtgacc
       61 atctatcgaa gcaagcgcaa gatggatgtg aaagtcgtac tgggcgaggc gcgcgagcgc
      121 acgtaagcgg gcgcaagccg atggcgacga tcttggcggc atccaacgtc agaatccgtc
      181 cccccctcgc ctccaagccg atacaacctg cacggccttg tcggcgccaa agcgattcgc
      241 gatagttgaa ctatacgcaa ccgctcgcgt atatgccaag gagatatatg cttgacgttc
      301 ctgaccgctc acgtatacta aattcatggc gaagaagagg aaaaaccctt atgctgtagc
      361 cctagggcgt aagggtggac gcaaaggtgg ccctgcgcgc gcggctaaca tgaccccaga
      421 gcaacgcagt gagagcgcga gaaatgctgt cacagcacgt tgggcaaaga ccaagaagca
      481 agccaactca actcttaaag ggtaatccgc tcttagccga agtgatgtct tggaatacga
      541 aacataggag agaacgattc taatggccga gcgaaggacc aagataccat ttccaacccc
      601 aaccagtccg ttgatggatg gaagcgaagt gggagtcgcc gaatctaccg agaagtggtc
      661 agaagttact ttggaggatg gcaccgtcat tcgcattaag ccgagtgtaa tctccgcgat
      721 tcgcatggac ggtcaatatg accccgaggg aaatccggta tatgcggtca aggccaacca
      781 tatcgtgacg ataaccagta cccctccaca cctgcgtaga ggcagcaagg acgcgaagat
      841 taattaaatt tatgacgcgc acacaatcaa tcacggcgga caagacagac atcattatgc
      901 tgacagcgta tgtcttgttg tatccgggca tcgctggagc aactagccaa caacagcaag
      961 agacgatttg tagcaatacg tctgagccat ccattgatgc ctctctcacg tcacctccag
     1021 attgtgtcgt ggatccgacg accaaaagat ggttgagatt tcagaacctt gttgatgagt
     1081 ggcgacatga gcgtggggcg atgtcttcga tcaccgagat ggcaacgctg cgcccttatc
     1141 agaacatcat cggcatgggc gaagaggcca ttccgctgat acttgcggag cttaagtccg
     1201 agggtgatga acccgatcag tggttttggg cgcttggggc catcactggg gctaatccgg
     1261 taagcccgga ggatcagggc aactttgcga aaatggctca ggcgtggttc aagtgggccg
     1321 aagatgaggg ccatgccggg tagctggcca ccgtacgagt tcccccatct gaacgatgac
     1381 acctgcgaag tcacaagcga agcttcaagg cgctataact gcgttgcttg ggcggctgga
     1441 gagaatattc gttggtggtg gcccgatccg atggggattg gatattggcc gcctgacgtg
     1501 cctagagccg taaccgtaga atcctttgtg gcggcatacg cagctttggg ataccagtta
     1561 tgttatgagg gctcccttgc ggcgggatta gaaaaaatag ccatcttcgg gaaagagaac
     1621 caaagcggct cagtggtccc tacacatgcg gcgctccagc tcgaatctgg cgaatggacc
     1681 agcaaactcg gtccgttcga ggatattcga catgataccg tcgatagagt aaacggaccg
     1741 gtctacggga aacttgtttg ctatatgtct cgcccacgcc aataagccgc gcgcaattca
     1801 tttgtcctcc gttgcttctc tcgcgatccg cgcagcggct tggctgcaat cctcgtgggg
     1861 tgctcgtttc ttgctgccta gggcggcatc atctcgccac gatcccaccc tgccgcatcc
     1921 cgcgaccttc tgccggtggc cgcaaactca aactgacctc cactaccccg gcgtgagcac
     1981 gattgccccg gcggctgtgc ctcggctata gttttaatgt catgtacgac gacttccagg
     2041 ctgtcgatcg ctggacgggt aagcccgtgc actgtagata tcaggcgctg atcgttgcca
     2101 ttgctacgcg gcacgcggac gcggtcgaca ttaagttttt gctggaggat cggccggtct
     2161 ggattgcgct tcctcatccc gcgtgggtag agtatcgccg gcagacgggg aaagtcatca
     2221 ccgatccgat ggcggtccag atcgccggcc attatttgaa gtgggcaatc gaatcgggcg
     2281 aagataacgg tcgcgagatg tgcagcttga acgtacagga aacgctcgat catctgcagg
     2341 ctgttgtgga cgaagcccac caacttgtaa cccgctgatg tctcggtcag ggggcctgt
//
LOCUS       DASRKK010000571         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_635902, whole genome shotgun sequence.
ACCESSION   DASRKK010000571 DASRKK010000000
VERSION     DASRKK010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635902"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1590)
                     /locus_tag="VFC15_13305"
     CDS             complement(130..1590)
                     /locus_tag="VFC15_13305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM11176.1"
                     /translation="MPKLHLLLLGMLTVATVSAAAQAKPASPWGPETLQQLRHLHQAA
                     LEDDYAYRQAAHLTDNIGPRLTGSPQAAAAVEYVAAELRKLGLDVTLEPVSVPNWIRG
                     EERAALVSYPGQAPGTQQKIVVTALASVDNATPAEGITAEIVVVNNFAELAAMPREKV
                     AGKIVVFNERFDHQMAAAGFGLAAYGQAVAYRENGREAAAKQGAVAALVRSAGGAEFR
                     LPHTGETGYDPKLQSIPAAAVTAEDAELMARLAAQGPVRLHLLLTPKLLPNVMSYNVV
                     ADLKGSEHPNQVVIVSGHLDSWDLGTGAIDDAAGVAVSMETAQLLKQLHLQPKRTIRI
                     VAWMAEETGIFGGKAYAKQHANDVANHFAAIETDLGAGHPLGINVGGDETILKLLQPV
                     ADILATSAAGVLRSSDETGADIIPLKVAGVPTFSPIQDVRTYFDYHHTAADTLDKIDP
                     RELRENAAVVAVLAYALATMDAELPRKPQPVPDWLK"
     gene            complement(1653..>2241)
                     /gene="def"
                     /locus_tag="VFC15_13310"
     CDS             complement(1653..>2241)
                     /gene="def"
                     /locus_tag="VFC15_13310"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZM11177.1"
                     /translation="SMRLKIVSVGEAVLRQRARPLTVAEIRSSEIQQLIQHMRETMND
                     APGVGLAAPQVGFPLQLAVIEDKQEYFAGLTAEQIAERERRPVPFHVIINPVMHQQSA
                     VDVEFFEGCLSVPGFTAIVPRWSHVRVECLDETGAPRVIEASGWYARILQHEIDHLHG
                     TLYLDRMQSRTFSSIENHSRIWKNKTTAEVKAGLA"
BASE COUNT          416 a          688 c          671 g          466 t
ORIGIN      
        1 gctttctgct gtgcgtaagc aaaactgcaa atcaagaacg cagcagccac gccgatcaac
       61 ccgcggcgca acatcgatcg ttctctggat agtcgcatga tgaaaccaat ctctggggca
      121 attcgctgtc tatttcagcc agtctggcac aggctgcggc tttcggggca attccgcatc
      181 catggtggcc agcgcgtagg ccagcacagc gaccaccgcg gcattctcgc gcaactcgcg
      241 aggatcgatc ttgtccagtg tgtcagcagc cgtgtggtga taatcaaaat acgtgcgcac
      301 gtcttgtatg gggctgaagg tcggcactcc ggcgaccttg agcggaatga tgtcggcgcc
      361 ggtttcatcc gacgatcgca agaccccggc cgcagacgtt gccaatatat ctgcgaccgg
      421 ctggagaagc ttgaggatcg tttcatcccc gcctacatta attccgagcg gatgcccggc
      481 gcccaggtcc gtctcgatcg cggcgaaatg gttggccacg tcgttggcgt gctgcttggc
      541 gtaagccttg cctccgaaga ttcccgtctc ctcggccatc cacgccacga tgcgaattgt
      601 acgttttggc tggagatgca actgcttcaa cagttgtgcg gtttccatcg acacggctac
      661 gccggcggca tcgtcaatgg cgccggtgcc caagtcccac gaatccaggt ggccggagac
      721 gatcaccacc tggttaggat gttcgctgcc cttcaagtcg gcgaccacgt tgtaggacat
      781 cacattgggt aacaatttcg gcgtcagcaa caggtgcaga cgcaccggac cttgggcggc
      841 gagccgggcc atcagctcag catcttcggc agtaacggca gcagccggaa tgctctgcag
      901 ttttgggtcg tagcccgttt cgccggtgtg cggcagacga aattccgccc cacctgcgga
      961 gcgcaccagc gcggccacag cgccctgttt cgcggcagcc tcgcggccgt tctcgcgata
     1021 ggccaccgct tgtccgtatg ccgcgagccc gaatcctgct gccgccatct ggtggtcgaa
     1081 gcgttcattg aagacgacga tcttgccggc tactttttcg cgcggcatcg ccgcaagttc
     1141 cgcaaagttg ttgacgacaa cgatttcagc cgtgattcct tcggccggag tcgcgttgtc
     1201 caccgacgcc aacgcagtca ccacgatttt ctgctgggtg cctggcgcct ggccgggata
     1261 gctgaccagt gcggcacgct cctcgccgcg aatccagttc ggtacgctga ctggttccag
     1321 cgtcacgtcc agaccgagct tgcgcagttc ggctgcgaca tactcgaccg cggccgcggc
     1381 ctgcggcgaa cccgtcagac gcggcccgat gttgtccgtc aggtgcgccg cctgccgata
     1441 ggcatagtcg tcctcaagag cggcctggtg caaatggcgc aattgttgca gggtttcggg
     1501 accccaaggc gatgcaggtt tcgcttgcgc tgctgcggag acggttgcga ccgtcaacat
     1561 ccctagcaag agtaagtgca attttggcat cagctttttc cagcacagtc cgaggtgagc
     1621 atagcaaatc gccacggggt ttgtagtgag gctcaggcga ggcccgcttt cacttcggcc
     1681 gttgtcttgt tcttccagat gcggctgtgg ttctcgatgg aactgaaagt acgactctgc
     1741 atgcggtcca ggtacagtgt cccatgcagg tgatcgattt catgttgcag gatgcgggcg
     1801 taccatcccg aagcttcaat gacgcgcggc gcgccagtct catccaggca ctccacgcgc
     1861 acgtgcgacc agcgcggcac aattgcggtg aagccgggca cgctgaggca tccctcgaag
     1921 aactctacat cgactgcgga ctgctggtgc attaccgggt taatgattac atggaaggga
     1981 accggccggc gctcgcgctc agcgatctgc tcggcagtca gaccggcaaa gtactcctgc
     2041 ttgtcttcga tgacggcgag ctgtagcggg aaccccacct gtggcgcggc caggccgaca
     2101 ccgggcgcgt cgttcatggt ttcgcgcatg tgctggatca gttgctgaat ttccgaactg
     2161 cgaatctcgg caacggtcag aggccgcgct cgctgtcgca gaaccgcttc accaacgctg
     2221 acaatcttga ggcgcatgct c
//
LOCUS       DASRKK010000572         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1027602, whole genome shotgun sequence.
ACCESSION   DASRKK010000572 DASRKK010000000
VERSION     DASRKK010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027602"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFC15_13315"
     CDS             <1..250
                     /locus_tag="VFC15_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11178.1"
                     /translation="QHSGKYSRYPYNDFLKDWGPSGEWGIIKSHHIEGAAVPKGHSGS
                     PFATASGVVVVVTVNDRVSDKAHIWVQNDDKTLGFSPY"
     gene            377..2086
                     /locus_tag="VFC15_13320"
     CDS             377..2086
                     /locus_tag="VFC15_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11179.1"
                     /translation="MTRNKAGIVGSSMLAVSALLLCGLQLSAQDNPKPTLTHATAYGV
                     TEPLGELAKIPQPVQYGFHLANPVRRIPKKNFGSAVDTAERNATFPSEFSIVKNFLGV
                     GNGFPGYTVGAEPPDTNMAVGGTQVLQWVNLSFTVCDKSTTTCGRAISGNTLWQQGIP
                     GTLCANSNDGDPLAQYDRMAHRWVLSQNVFVSPYATCIAISDTDDANGTWHVWQFPVP
                     GNGFPDYPKIGVWPTSGVNNGYYQAQNNFGPGGSGFMGPQICAYDRHALLTNTAPFTQ
                     ICFQLSASEDSLLPGDLDSILGVPNTPAGTPQDEFYIGSVADVDNSHLSLYQFHADFA
                     TPANSFVLGSPNTILQTVPTYSGSCHGAFGGACVPQLGTTALLDSLGDRLMYRFAYWN
                     DGPAPSAAPCAICATPHFQHWYVNGDVESSGGEIGVRWYEFRAPPVPGIGTLTSTTLT
                     TFQAGTYAPDSNWRWMGSVAQDKKNDILVGYSESSSTIHPAIGLAGRKTSDPVSQLSD
                     EVLVPINNNGSQTSSRWGDYSSMRIDPVDNCTFWYTTEYYMVTASFDWSTQIVSARFS
                     GCQ"
     gene            complement(2175..>2450)
                     /locus_tag="VFC15_13325"
     CDS             complement(2175..>2450)
                     /locus_tag="VFC15_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11180.1"
                     /translation="LMCNVDASLVTTAEQARDALIRQVTGAVQWDKSMRGLAALPVHN
                     FAEVGPGKVLCGLMRQIDRSRSCVNVEDEASLQKTVNHFSAASAKPD"
BASE COUNT          511 a          744 c          675 g          520 t
ORIGIN      
        1 acagcattcc ggcaagtatt cgcgttatcc atacaacgac ttcctcaagg attggggacc
       61 gagtggcgaa tggggcatca tcaaaagcca tcacattgag ggcgcagcgg tgccgaaggg
      121 ccacagtggc tcgccgtttg ctaccgccag cggcgttgtg gtggtggtca ccgtcaacga
      181 ccgggtcagc gacaaagcgc acatctgggt gcagaacgac gacaagacgt tggggttctc
      241 gccgtactag ctgcgagact tttcttttcg cttttcctcc tggcccgcgt gctggaatcc
      301 tgataggtgc cacaggaaca aattctagga catggaaacc gcggagacga attgagaaaa
      361 aggaagggtg ttaacaatga ctagaaataa agcagggatt gtgggatcgt ctatgctcgc
      421 cgtgagtgct ctcttactct gtggcttgca gctatcagcg caggacaacc cgaaaccgac
      481 gttaacccat gcgaccgcct atggcgtcac ggaaccactg ggagaactgg ccaaaattcc
      541 ccaaccggtg caatatggct tccaccttgc caatccggtt cgtcgcattc ccaaaaagaa
      601 ctttggatct gcagtggaca ccgcggaacg aaacgctact tttccttcgg aattctccat
      661 cgtgaagaat ttccttggag tcggcaacgg cttccccgga tacaccgtgg gtgccgagcc
      721 gcccgatacc aacatggcgg tgggcggcac ccaagtcctg caatgggtca atctttcctt
      781 cacggtatgt gacaaaagta caacgacgtg cgggcgagcc atctcaggga acacgctgtg
      841 gcagcaaggc atccctggga ccttgtgtgc caacagcaat gacggcgacc cgctggcgca
      901 gtatgaccgc atggcccatc gctgggtcct gtcgcagaac gtctttgttt cgccctacgc
      961 gacctgcatc gctatttccg acaccgacga cgccaatggt acctggcacg tttggcagtt
     1021 cccggtgccc ggtaacggct tccccgacta tccgaagatc ggcgtttggc cgaccagcgg
     1081 cgtcaacaac ggctactacc aggcccagaa caactttggt cctggcggca gcggcttcat
     1141 gggtcctcag atctgtgcgt atgaccgcca cgcattgctg acaaacacgg ccccttttac
     1201 ccagatttgc ttccagttgt cggcaagcga agacagcttg ttgcccggcg acctggattc
     1261 cattcttggt gtgccgaaca cccctgcggg aactccacaa gatgagttct acatcggcag
     1321 cgtagccgat gtggacaatt ctcatttgtc gctctaccag ttccatgcgg actttgccac
     1381 tcccgccaat tcgttcgttc tgggtagccc caatacaatc ctgcaaaccg tacctacgta
     1441 cagcggctcc tgccatggtg catttggtgg cgcttgcgtc ccgcagcttg gtacgaccgc
     1501 tttgttggat tccttgggcg atcgcctaat gtaccgtttc gcctattgga atgatggtcc
     1561 cgcgcccagc gccgcaccgt gcgccatttg cgcgactccc cacttccagc actggtatgt
     1621 aaacggagat gtggaatcct ccggtggaga gattggcgtc cgctggtacg agttccgggc
     1681 tccgccggtg cccggtatcg gcactctgac gtctaccacg ctcaccacgt tccaagcggg
     1741 cacctatgct cctgactcca attggcggtg gatgggctct gttgcccaag acaagaagaa
     1801 cgacatcctc gtaggctaca gcgaatccag ttcgaccata catccggcaa tcggcctcgc
     1861 tggccgcaag acgagcgatc cagtcagcca actgtccgat gaggtactgg tcccaattaa
     1921 caacaacggc tcgcaaacct ccagccgctg gggcgactac agctccatgc ggatcgatcc
     1981 ggttgacaat tgcaccttct ggtacacgac cgagtactac atggtgactg caagcttcga
     2041 ttggagcacg cagatagtgt cggcaagatt ctccggctgc caataacgga ggctgttcgc
     2101 cattcgctct tggctagtca ggcggagcta catccgccac accgcagcga atgagcgaca
     2161 tcgccgtaag cgagttagtc cggtttcgcc gacgccgcgc tgaagtggtt taccgtcttc
     2221 tgcagcgagg cttcgtcttc cacgttgacg cagctgcgcg agcgatcaat ctggcgcatg
     2281 agtccgcaga gcaccttgcc ggggccaacc tcggcaaagt tatgcacggg gagcgctgcc
     2341 agtccgcgca tggacttgtc ccactgtact gcccccgtta cctggcgtat gagcgcatcg
     2401 cgcgcctgct cggcggtcgt gactaggctc gcgtccacgt tgcacatcaa
//
LOCUS       DASRKK010000573         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_734749, whole genome shotgun sequence.
ACCESSION   DASRKK010000573 DASRKK010000000
VERSION     DASRKK010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734749"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..894)
                     /locus_tag="VFC15_13330"
     CDS             complement(94..894)
                     /locus_tag="VFC15_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM11181.1"
                     /translation="MRRSSVRFQTKPEIALAQIRWLVEEGVAHAPVLADAAYGNDNGF
                     RDGLQQLHLEYAVGVQSSTSVWPPGLAPLPAKVGGGMGRPPKLLRREAGHAPLSVKDL
                     ALCLSPSDLRRVSWREGTRGTMHSRFAALRVRVAHRNYERSQPRPEQWLLMEWPKTEK
                     EPTKYWLSNLPASISMRKLVATAKLRWRIERDYEELKQELGLGHFEGRNWRGFHHHAS
                     FCIAAYGFLVVERCLFFPTPDSPPLRAANLQLRLPRLPHGAKPRGAAD"
     gene            complement(1346..1555)
                     /locus_tag="VFC15_13335"
     CDS             complement(1346..1555)
                     /locus_tag="VFC15_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11182.1"
                     /translation="MIVWLRHFLGWLRSAFCTREDLILENLALRQQLLALHAQRPRRR
                     LVTSQKLFWVVLRRLWARWKNLSSW"
     gene            complement(1707..2582)
                     /locus_tag="VFC15_13340"
     CDS             complement(1707..2582)
                     /locus_tag="VFC15_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZM11183.1"
                     /translation="MFRFGAFELNEATGELRKQGIRIKLHSQPFQVLVMLLERPSELV
                     TREEMRQRLWGEDTFVDFDHGLNTAVNKLREALGDLASQPRHVETISGRGYRFIAPVT
                     LAGSISRVSEVPIAAACDAVASAGNGEPVLPVAPLDHGLETLRWTLLAAPHELPRAPR
                     TLVRTLLMLIQAMYLAFYLAALGNIPEIHAIFWDARLPSPGILITLLVTTAVVLIPVR
                     LFLFAAVTFDFQQLSTKFDKLFPVLLLMDLLWALSPFLLVHHLSLGLALGMTAALVYM
                     PFAQRSLVLMCARDR"
     gene            2981..3691
                     /locus_tag="VFC15_13345"
     CDS             2981..3691
                     /locus_tag="VFC15_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11184.1"
                     /translation="MEKRLAQQHVSPDTYLAVLNLGAVERTEAERQLRSGDLRVEPVD
                     GGAQEVSGGLLHHWRGAALVPGASAKDMLALLRDYYHLAMYYAPEVESSHVMSDHGGV
                     ATVAIRMRKQKVVTVVLDTEYDVHAGLTGVSGYGFSRSTHIWQIAEAGTAHERRLPEG
                     KDDGFLWRSNSYWSFLELPDGLLIECEAVSLTRDVPSGSAWLITPIIENIPRESLTFT
                     LSATRKALESNPLKEANQ"
     gene            3688..4302
                     /locus_tag="VFC15_13350"
     CDS             3688..4302
                     /locus_tag="VFC15_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11185.1"
                     /translation="MNEGWEVQARGAMLADGRQSGFAIARMVLNAWTATLRHEVTLYR
                     EERFASILDPIRASLFIVLLACLFPAYIAAHGLDMGESLQAASPETHPAIRTIYVKAE
                     GAETLAAAKARNLLASPKCYTITALEDQADAILSISSEKQGASSIFTHIPFTGQKIYT
                     SATLFDRKTGEVLWTTDWSDIWFGSPAKAGERVVQRLKKDRQCQ"
     gene            4361..5083
                     /locus_tag="VFC15_13355"
     CDS             4361..5083
                     /locus_tag="VFC15_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZM11186.1"
                     /translation="MNEGCEVQVRESMLADRRQSGFVHALRVHKSLTAEVEKRALVWM
                     AERTPKAINSDHLTTLGFVSQLLAGAAYALAAWDPRALWLVNVFLVLNWLGDSLDGTL
                     ARVRNQQRPRYGFYVDHMADTFGALALMAGLACSGYVHGQISAGMLVCFYVLSIESYL
                     ATYTMGHFHLSHGIFGPTEIRILLAVGNAVLLVHPYVEVASQRFLLFDIGGAVAIAGM
                     AFMAATAAIRHTVVLYREEELP"
     gene            5080..5490
                     /locus_tag="VFC15_13360"
     CDS             5080..5490
                     /locus_tag="VFC15_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZM11187.1"
                     /translation="MTSAGRLLARLGKFNLVGFLGAALQLLLLYVLTKCFHMSAVTAT
                     PVAVEIVVLHNFVWHERFTWRDRQLSSVRQRTARLWRFHAGNGLTSLLSNTALAYFLV
                     ERLKAPVLPSAMGAIILCSLINFFVADRWVYTTT"
BASE COUNT         1234 a         1609 c         1685 g         1103 t
ORIGIN      
        1 aggcagcagg gacatctggg cagagaacga gccaggtggg ttgcgatctg gcggcgcagg
       61 gtggcgatgg aatgcggatt atggcgttcc gttctaatcg gcagcgccac ggggtttcgc
      121 accgtgtggc aggcggggta ggcgaagttg gaggttggcg gcccgcagcg gtggggagtc
      181 gggcgtgggg aaaaaaaggc atcgttccac caccaggaag ccgtaggcag caatgcagaa
      241 gctggcatgg tggtgaaagc cgcgccagtt ccgtccttcg aagtgcccca gtccaagctc
      301 ctgcttcagt tcctcgtagt cgcgctcgat gcgccaacgc agcttcgcgg tggccaccag
      361 tttccgcatg ctgatcgacg ccggcaggtt ggagagccag tatttcgtcg gctccttctc
      421 ggtcttgggc cattccatca gcagccattg ctctgggcgc ggttggctgc gctcgtagtt
      481 ccgatgcgcc acgcgcaccc gcaaggccgc aaagcgggaa tgcatcgtgc cgcgcgttcc
      541 ttcacgccaa ctgacccggc gcaggtcgct cgggctcaga cacaaggcta gatccttgac
      601 cgaaagcggt gcatgccccg cctctcggcg aagcaacttc ggcggacgtc ccattccgcc
      661 gccaaccttg gcggggagcg gagccaatcc cggcggccac actgaggtcg aggactgcac
      721 gcccacggca tattccagat gcagttgttg caggccgtcg cggaatccgt tgtcgttgcc
      781 ataggctgcg tcggccagca ccggggcgtg cgccacaccc tcttcgacca gccagcgaat
      841 ctgtgccagg gcaatctcag gcttggtttg aaagcggact gatgatcgcc gcattcccaa
      901 gtttcgtgca gctgcaaggc aacccaaagc gcgttcggat tgcaagtcac gttgcggtgt
      961 aacactcgac gccggtcatg gctgatcacg aaaaagcagt acagaatgcc gaaggtcagt
     1021 gtcggcacgg tgaagaaatc catggcggca atggcctcgc tatgattccg aaggaaggtg
     1081 agccagcgct tcgtggggtc caccggtctc ggggctcgcc gaagccatct cgaaaccgtt
     1141 ggttccgaca gatcgaagcc cagcttgagc aattcgccgt gaaggcgtgg cgctccccac
     1201 gttggattct ctgcaaccat ttgaaaaatc aaggctcgaa tttctttact cagagacttg
     1261 cgtcctcccc tcgaccgggc tcttgaaagc caattccaat acagccgaaa gccggcgcga
     1321 tgccgggaga ctacagtcct gggtgtcacc aggatgagag gttcttccat cgagcccata
     1381 gcctgcgcag tacgacccag aacagcttct gcgaagtgac aagtcgccgg cgaggccgtt
     1441 gggcgtggag agccagcaac tgctgacgca gagcaaggtt ttcgaggatg agatcctcgc
     1501 gggtacaaaa ggcactgcga agccagccga ggaagtgccg gagccacacg atcatggcaa
     1561 gaatcttatc cgacgttgct aatccagcca tcgcataagt cattgatatt ggggatgtac
     1621 gatgttttga tgaatgacag ccctgcattc ccgatgcgct cttaccggca gagagccaca
     1681 gagctcataa gtccacgttg gtctgatcat cggtcccgcg cgcacatcaa aaccagcgag
     1741 cgctgagcaa acggcatgta gacgagtgca gcggtcattc cgagagccag acctaggctc
     1801 aggtgatgaa caaggagaaa cggagacaaa gcccacaaaa gatccattaa tagcagcacc
     1861 ggaaagagct tgtcgaactt tgttgaaagt tgctgaaagt cgaacgtaac cgccgcaaaa
     1921 aggaacagac gaactgggat cagtaccacg gctgtcgtca ccagcagcgt tatgaggatc
     1981 ccaggtgatg gcagccgcgc gtcccaaaaa atagcatgga tttccgggat gttcccaagc
     2041 gccgccaggt agaacgccag atacattgcc tggataagca tcagcagagt ccgcaccagc
     2101 gttctgggag cgcgaggcag ttcgtggggg gccgcgagaa gtgtccatcg caatgtctcc
     2161 agcccatggt ccagaggagc gaccggcaag acaggttcgc cgtttccagc cgacgcaacc
     2221 gcatcgcatg ccgcggcgat tggcacctcc gagaccctcg aaattgagcc cgcgagagtc
     2281 actggcgcta tgaaacgata gcctctgccg gagatcgtct caacatgtcg tggttgggag
     2341 gctaagtccc cgagcgcctc gcgaagcttg ttgacggcag tgttcaggcc gtggtcgaag
     2401 tccacaaacg tgtcttcgcc ccaaaggcgc tgccgcatct cttcgcgggt caccagctcc
     2461 gagggccttt cgagaagcat cactaatacc tgaaatggct gcgaatgcag cttgatgcgg
     2521 atgccctgtt tacgaagctc tcccgtggcc tcgttcagct cgaaggctcc gaagcgaaac
     2581 acgcggcttg ttacctcccg tggcattggc acggttccag tctagctgcc tttggcggcc
     2641 cggagccagt gggagaaccc tcccggcata acgtgaagct ctgggtggaa tgattacagc
     2701 ctcactcagt tccgcccggg caaacaaaca gcttccgcca caggaagata acttcctata
     2761 accctcggcc acgcagtcga ttacacgatg attcacaagt gaggaacgtg tgaggcgtca
     2821 tgcattgcat ctgccgctcg cctcatgcat cctgcggcta cgaggcaaat tatgcaagtg
     2881 cggatgcgga cagcggcgat catcctccta cttgcagccc cctgcccggc ggcccggttg
     2941 accacctgcc cagcaggcat ttgaaaatta tgcagccgct gtggagaagc gattagccca
     3001 gcagcatgtc agccctgaca cctaccttgc ggtgctcaac ctcggagcgg tcgagcgaac
     3061 tgaggcggag cgtcaactga gatcgggtga cctccgagtg gaaccggtag atggcggtgc
     3121 acaggaagtc agcggaggac tgttgcacca ctggcgtggc gcggcattgg tccccggcgc
     3181 aagcgccaaa gacatgctgg cgctgttacg cgattactac catcttgcca tgtactacgc
     3241 gcccgaggtt gagtcttccc atgtcatgtc ggatcacgga ggggttgcaa ccgtcgcaat
     3301 tcggatgagg aagcagaagg ttgtcacggt cgtgctagac acggagtacg acgtccatgc
     3361 agggctgacc ggcgtcagcg gctacggttt ctcgcgcagc acgcacattt ggcagatcgc
     3421 ggaagcgggc acggcgcacg agcgcaggct gccagagggt aaggatgacg gcttcctgtg
     3481 gcgctcgaac tcttactgga gcttcctcga actgcccgac ggactgctca tcgagtgcga
     3541 ggcagtttcg ctgacgcgcg atgtcccaag tggctcggcc tggctgatta cgccgatcat
     3601 cgagaacata ccgcgggaat cgctgacctt tacgctgagc gctaccagaa aggcgctcga
     3661 atcgaaccct ctcaaggagg caaaccaatg aacgaaggat gggaggttca agcaagagga
     3721 gcaatgcttg ccgatgggag acagagtggc tttgccattg ccagaatggt actaaatgct
     3781 tggacagcca cactccgtca cgaggtcacg ctttatcggg aggaaagatt tgccagcatc
     3841 ttagacccaa taagagcttc acttttcatt gttttgctag cctgcttatt tccagcgtac
     3901 atagcggcac atggcctgga catgggggaa tccctgcaag ccgcatcgcc tgaaacccat
     3961 cctgcaatcc gcacgattta cgtgaaagcc gagggagcgg aaacgttggc ggcagccaag
     4021 gccaggaatc tattagcaag ccccaaatgc tacaccataa ctgcactaga ggaccaagcc
     4081 gacgcgatat tgagcatcag cagcgagaag cagggcgctt cgtccatctt tacgcatata
     4141 ccctttacgg gccagaaaat ctacacgagc gccacgctct ttgatcggaa gacgggtgag
     4201 gtgctctgga caacagattg gtcggatatt tggttcgggt caccagccaa ggcgggagag
     4261 cgcgttgtac agcgtctcaa gaaggaccgg caatgccaat gagcggccgg ccatgcacac
     4321 gcaacccact cggatcaacc cctcttaagg aggcacagca atgaacgaag gttgcgaggt
     4381 tcaagtgaga gaatccatgc ttgccgatag gagacagagt gggtttgtac acgcgctgcg
     4441 agtccacaag tcactgaccg cggaagtcga aaagcgcgca ttagtatgga tggccgagcg
     4501 gacaccgaag gccatcaatt cggaccattt gaccacctta ggctttgtgt cgcagctctt
     4561 ggcgggtgcg gcctacgcgc tggcagcgtg ggacccgcgg gctctgtggc tcgtgaacgt
     4621 cttcctcgtt ctcaactggc tcggcgacag tctcgatggc acgctggcgc gagtccgcaa
     4681 ccagcagcgc ccacgctatg gtttctacgt ggaccacatg gccgacacct tcggcgcgtt
     4741 ggctctgatg gcaggactcg cgtgctccgg ctacgtgcac gggcagatat cggccggcat
     4801 gctggtgtgc ttctacgtac tctccattga gagctaccta gcgacctata caatggggca
     4861 ttttcatctt tcgcacggca tctttggtcc taccgaaatt cgcatcctgt tggcggtggg
     4921 aaacgccgtg ctcctcgtgc atccctatgt ggaagtcgcg agccagcgct ttctgctgtt
     4981 cgacatcgga ggcgctgtgg ccattgccgg aatggcgttc atggccgcca cagccgcaat
     5041 tcgacacacc gtcgtgctgt acagggaaga agagctgcca tgaccagcgc cgggcgactg
     5101 ctggcgcggc tcggcaaatt caacctggtg ggcttcctgg gcgcggcatt gcagttgctt
     5161 ctactttatg tgttgacaaa atgcttccac atgtctgcgg ttaccgctac gccagtggcg
     5221 gtggagatcg tggtgttgca caactttgtg tggcacgaac ggttcacatg gcgcgatcgt
     5281 cagctttcca gcgtccggca aagaacagcc cggctctggc gatttcacgc cggaaacggt
     5341 ctcacctcac tgctaagcaa tacggcgcta gcctactttc tggtagaacg gctcaaggcc
     5401 ccggtgctgc cgtcggccat gggagccatc attttgtgct cgctcatcaa tttcttcgtt
     5461 gcggatcggt gggtatacac gaccacgtaa catccaaaaa cactgattcc ctgatgcggg
     5521 aaaggcgaac tgaagtccag aaagattaaa ttgtgtggct ggtggctggc aaagttgatt
     5581 ggtcccgatc aacgatagat ccctatcggg gactgccccg aaatgtgggg t
//
LOCUS       DASRKK010000574         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_831932, whole genome shotgun sequence.
ACCESSION   DASRKK010000574 DASRKK010000000
VERSION     DASRKK010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831932"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFC15_13365"
     CDS             complement(<1..133)
                     /locus_tag="VFC15_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZM11188.1"
                     /translation="MEQGTVKWFNDAKGYGFISRQNGEDVFVHYSAIQAGGFRSLQEG
                     "
     gene            407..805
                     /gene="acpS"
                     /locus_tag="VFC15_13370"
     CDS             407..805
                     /gene="acpS"
                     /locus_tag="VFC15_13370"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZM11189.1"
                     /translation="MLYGDGMIVGTGIDIAEVDRIAQTIARFGGRFKERVFTADEIRY
                     CESKANKAERYAARFAAKEAGMKAIGTGWSRGVTWRDIEVRRLPGGRPTLVFHGKAGE
                     LFAKLGGVRSHLSITHTKESAMAYVIIESE"
     gene            1218..1919
                     /locus_tag="VFC15_13375"
     CDS             1218..1919
                     /locus_tag="VFC15_13375"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM11190.1"
                     /translation="MAQIFHRSANSLSRLSILAVVVVVTVLLWAALETQRAPYVTYQN
                     VAQSQPVPFSHQHHNAGLGIDCRYCHTSVEQSSYAGIPPTKTCMNCHSQIWTNAPMLE
                     PVRESYRTGNSLRWTKVNYLPDYVYFNHSIHVNKGVGCLICHGPVNKMPLMYQASSMR
                     MEWCLECHRAPEKFLRPKDQVFNMNYEEPTDLHPVKLADGTAYTDQIALGSALKTQYN
                     VRTVQDITSCNTCHR"
     gene            2028..>3550
                     /locus_tag="VFC15_13380"
     CDS             2028..>3550
                     /locus_tag="VFC15_13380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HZM11191.1"
                     /translation="MKIDLRKGSGTDEKMDKESQNKSEAVCPSKLELAEVRDQLARAK
                     SGPQYWRSLEELSQTEGFFEMVHREFPRQASVWPEGASRRDFMKLMTASMALAGLSAC
                     VKQPLEPIVPYVRQPEPITLGLPLFFASAMPFAGYATPVLVESHEGRPTKVEGNPDHP
                     ASLGGSDVFAQASILDMYDPDRSQVITYTGDIRAWSDYANAIKGPLNSQKAVKGGGMR
                     LLTRTVSSPTLAAQINAVLTAYPEAKWVQYDPLNRDNARAGAQLAFGQYVETRYALDK
                     ADTILSLDGDFLSSGYPGFLGYARQFSSRRNPELKEKMSRFYMVESTPTNTGGKADHR
                     LPVRASEVEQVARAIAAGLGVGSGTAKAEQQKFVAALVKDLQAHKGAALVIPGDNQPP
                     AVHALAHAMNQALGAVGTTVFYTDPVEAKPQDQTAALKELVGEMNAGKVDLLLIVGGN
                     PVYDVPADLKFADAMGKVQLRVQHSLYQNETTDFVHWHINATHYLEQWGDVRSIDGTA
                     "
BASE COUNT          802 a          903 c         1155 g          690 t
ORIGIN      
        1 gaccttcctg caagctgcgg aatccgcctg cctggatggc agagtaatgg acgaaaacgt
       61 cttcgccgtt ctgacggctg atgaagccgt agcccttcgc gtcattgaac cacttaactg
      121 ttccttgttc catggtgatg catgttcctt caaattgatg tagctacaat tcgaccggat
      181 tgtggagcga gcagggatgc tgcaaggcag gaactgctgc gtacaacgtc gatcaatcga
      241 gcaagataat aatagcagtg taactgcgaa aggccaaaca gtttatgggt acaaaccagg
      301 gtggacaggg tggatagcgg tgcaaatgat taaaacttaa ggctattacg acgatttcga
      361 cggttgatag tagcgccatg ttcaaaatgg gaatcgggtt gcaagaatgt tgtatggtga
      421 tggcatgatc gtgggcacgg gcattgatat cgcagaagtg gatcgaattg cgcagaccat
      481 cgcgcgtttt gggggccgct ttaaggagcg ggtcttcacg gccgacgaga tccggtattg
      541 cgaatcgaaa gccaacaagg cggagcgcta cgccgcgcgc ttcgctgcca aagaggcggg
      601 catgaaggcc atcggaacgg ggtggagccg gggggtgacc tggcgggata tcgaagtgcg
      661 acgtttgcct ggcggacgac cgacattggt cttccatgga aaggccggag aacttttcgc
      721 caagctcggg ggagtgaggt cgcatctgtc gatcacgcat acgaaggagt cggcgatggc
      781 atacgtgatt attgagagcg agtaaaggaa gtttcgagtt tcgagtaaag caggttcctc
      841 cactcgccgc cgaagcggca gctcccgtcg ggatgacgaa actcaaggaa ataccgtcca
      901 caaagacggt ttcgagtttg tagtttcaag tttcgagtaa ggaccggagt ttcgctttgc
      961 aagcaaaccg tttcagcgaa gactaatgtt gtgattttca acgatgaaac cgtgccattg
     1021 tgggaatttg caccaacatc aagtcataaa cgcgcaaatc aaaatgaatg cttgcaaagg
     1081 ggttaacctg acttgttata atcacgcact tgagtgattt gcgtcacata gatagggacg
     1141 taagggccac aactgcacga atccggcgcc gaatggggta atcggctcct tacccgcaac
     1201 actggaaagc ttcgtacatg gcacaaattt ttcatcgaag tgcgaatagc ctttcgcggc
     1261 tgtcgattct cgctgtggtc gtggtggtga ccgtgctgct atgggcggcg ctggaaaccc
     1321 agcgggcgcc gtacgttacc taccagaacg tggcccagtc gcaaccggtg ccgttcagcc
     1381 accagcacca taatgcgggc ctggggatcg attgccggta ctgccacacc tcggtggagc
     1441 agtcgagcta tgccgggatc cctcccacca agacctgcat gaactgccat tcgcagatct
     1501 ggacgaacgc cccgatgctg gagccggtgc gggagagtta ccgcacgggc aattcgctgc
     1561 ggtggaccaa ggtcaattac ctgccggact acgtttattt caaccacagc attcacgtga
     1621 acaagggtgt gggctgcctg atctgccatg gtccggtcaa caagatgccg ctgatgtacc
     1681 aggcgtcgtc gatgcgcatg gaatggtgcc tggaatgcca tcgcgcgccg gaaaagttcc
     1741 tgcgtccgaa ggaccaggtg ttcaacatga actatgagga gccgaccgat ctgcatccgg
     1801 tgaagttggc ggatggcacg gcgtacaccg accagatcgc gctgggatcg gcgctcaaga
     1861 cgcaatacaa cgttcgcacc gtgcaggaca ttacgagttg caacacctgc catcggtaga
     1921 gatgagcggt gacttgcctc gcggactaaa gtccgctcgg catatcgaaa ataaggggct
     1981 tgtacggcac ggctaaagcc gtgccccttc aaaacggtgg catgagggtg aagatcgacc
     2041 tacgtaaggg aagcggaacc gacgaaaaga tggataaaga gagccaaaac aagtccgagg
     2101 cggtctgtcc cagcaagctg gagctggcgg aagttcgcga tcagttggcg cgagctaaga
     2161 gcggcccaca gtactggcgg agcctggaag agctgtcgca gaccgagggg ttcttcgaga
     2221 tggtgcaccg cgagtttccg cggcaggcgt cggtgtggcc cgagggagcg tcgcggcgcg
     2281 acttcatgaa gctgatgacg gcttcgatgg cgctggccgg gctgtcggcg tgcgtgaagc
     2341 agccgctgga gcccatcgtc ccttacgtgc ggcagccgga accgatcacg ctgggcctac
     2401 cgttgttttt tgccagcgcg atgccgttcg cgggatacgc gacgccggtg ctggtggaga
     2461 gccacgaggg acgtccgacc aaggttgagg gtaatccgga tcatcctgcg agtttgggtg
     2521 gaagcgatgt tttcgcgcag gcgtcgattc tcgacatgta cgatcccgac cgctcgcagg
     2581 tgattacgta cacgggtgac atccgggcct ggagcgacta cgcgaatgcg atcaaggggc
     2641 cgctgaattc gcagaaggca gtcaagggcg gggggatgcg cctcctgacg cgaacggtga
     2701 gttcgccgac gctagcggcg cagattaatg cggtgctgac ggcgtatccc gaggcgaagt
     2761 gggtgcagta cgatccgctg aatcgcgaca acgcacgcgc gggcgcgcag ttggcgtttg
     2821 ggcagtatgt cgagacgcgc tacgctctcg acaaggcgga cacaattctt tcgctcgatg
     2881 gcgattttct ttccagcggc tatccgggat ttttgggata tgcgcggcag ttctcgtcgc
     2941 gacgcaatcc tgagctgaag gagaagatgt cgcggttcta catggtggaa agcactccga
     3001 ccaacacggg cggtaaggct gaccaccggc ttccggtgcg ggcatcggaa gtggagcagg
     3061 tggcgcgggc catcgcagcg ggacttggcg tgggcagtgg gacggccaag gcggagcagc
     3121 agaaatttgt tgcagcactg gtgaaagacc tgcaggcgca taagggcgcg gcgttggtga
     3181 ttccgggaga caatcagcct ccggcggtgc acgcgctggc gcatgctatg aaccaggcgc
     3241 tgggcgcggt gggcacgacg gtgttctata ccgatccggt ggaagcgaag ccgcaagacc
     3301 agacggccgc gctgaaagaa ctggtcggcg agatgaacgc cgggaaggta gacctgctgc
     3361 tgatcgtcgg cggaaacccg gtgtatgacg tgccggcgga cctaaaattc gcggacgcga
     3421 tgggcaaggt ccaactgcgg gtgcagcaca gcctttatca gaacgagacg accgatttcg
     3481 tgcactggca catcaacgcc acgcattacc tggagcagtg gggcgatgtg cgtagcatcg
     3541 atggaacggc
//
LOCUS       DASRKK010000575         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_929911, whole genome shotgun sequence.
ACCESSION   DASRKK010000575 DASRKK010000000
VERSION     DASRKK010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929911"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..555)
                     /locus_tag="VFC15_13385"
     CDS             complement(385..555)
                     /locus_tag="VFC15_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11192.1"
                     /translation="MADLPIDADQVIRQAPEPAIFRDFILRFTESRDGWKALRHRLTI
                     NLLGELVMRPMP"
     gene            693..1967
                     /locus_tag="VFC15_13390"
     CDS             693..1967
                     /locus_tag="VFC15_13390"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HZM11193.1"
                     /translation="MESVETAIRAAMHQAGATALTQLLQFEQPDAEHLMVPCVCGHTA
                     RFKELRSKTFLSVVGAVEVRRPYYLCSHCSKGQHPVDGELGVDGLESSPGVRRMEAVV
                     GSEMPFAPACEPMKVLAGLEVPAKAIERAAESIGAAIARSDEREIDRAKQLVLPIVSR
                     QNIPKMYVLMDGVQIPVVAAETAGRIGRVEGQRARTRECKLGAVFTQTTVDQDGWPIR
                     DPDSTSYVGAVETADAFGYRIYTEAWRRGWEWATTKIVIGDGAVWIWNLADQHFPGAV
                     QIVDLYHARQHLWDIAALLYPHDAAAKKRWMAPLKDLLDHGRTELLVEWLREIAAEHS
                     RTQPGLAEEISQQADYFETNTHRMQYPEFREQGFFVGSGVIEAGCKSIVGVRLKQSGM
                     FWTVRGANAIIALRCCRFNHRFEDFWAETRAA"
BASE COUNT          457 a          618 c          618 g          405 t
ORIGIN      
        1 gatctggctg cggttaaagc caattgatca gtttcgaccg agccaacggc aacgcttagc
       61 ggaaactgaa acccgtcgcc gacgatgctg gccattctct gcaactgcac attttcctct
      121 tccgtggtga agagcaggca gtttagggtg tgcgacataa aagttcaggg attgttggat
      181 tctctttttt tcgccccgaa tctgaaagta cgcaatactt acaggtgagg agccgccatg
      241 cccgagccgc tttctgcatt gatcgaggaa cgttccctgc tgctacgcca ggttgccgaa
      301 ctgggagatt ttcagcccgg ttccatctca agccctaccc gcaagtgtgg gaaacccagc
      361 tgccattgcg ccaggcccga cgattcaggg catgggccgc attaccaact cacccaaaag
      421 gttgatggta agacggtgac gcagagcctt ccatccgtcg cggctctccg taaagcggag
      481 aatgaaatcg cggaatatcg caggttccgg agcctggcgg atgacctggt cggcgtcaat
      541 cggaagatct gccatttacg tccagtggaa cagaccgggc agacggacca ggaaaaaaaa
      601 cggcgcaaac gatccagcag gaagtcgccc gcgaagtaga gcaacttctg cgcgtcgttt
      661 tccgcgaccg caagacgacg ggcaggctcg atctggagag tgtcgagaca gcgatccgtg
      721 ccgcgatgca ccaggccggg gctacagccc tgacgcagct gttgcagttc gaacagcccg
      781 atgctgagca cctgatggtt ccttgtgtgt gcggtcacac ggcacgcttc aaggaactgc
      841 gcagcaaaac cttcctctcg gtagtcggtg ctgttgaggt ccggcgtccc tactacctct
      901 gttctcattg ctccaaagga caacatccgg tcgatggcga actgggcgtt gacgggctgg
      961 aatcttcacc cggcgtcaga cgcatggagg cagtggtcgg ctccgagatg ccgttcgcgc
     1021 cggcctgcga gccgatgaag gtactggcgg gtctggaagt ccccgccaaa gccatcgaac
     1081 gagccgccga gagtatcggc gcggcgattg cccggagtga cgagagggaa atcgaccggg
     1141 ccaaacaact cgtgctgccc attgtttccc gccagaacat tccgaaaatg tatgtactga
     1201 tggatggtgt ccagatcccg gttgtggccg ctgaaaccgc ggggcggatc ggcagagtcg
     1261 aagggcaacg agcccgcacc cgcgagtgca aactcggagc cgtgttcacg cagactaccg
     1321 tggatcaaga cggatggccc atccgcgacc cggattccac cagctatgtc ggcgccgttg
     1381 aaaccgccga cgcgttcggc taccgtatct acaccgaggc ttggcgcaga ggctgggagt
     1441 gggccacgac caaaatcgtc attggcgatg gagccgtctg gatctggaat ctcgccgatc
     1501 agcacttccc gggggccgtc cagattgtcg atctctatca tgccaggcaa cacctctggg
     1561 atatagccgc attactttat ccgcatgatg cagcggcgaa gaaacgctgg atggcgccgc
     1621 tgaaagatct gctcgatcat ggcagaaccg aactactcgt ggagtggctg cgcgaaatcg
     1681 ccgccgagca ctcccgaacc caacccggcc tggcggagga aatcagccaa caggccgatt
     1741 acttcgaaac caacacgcat cggatgcagt acccggagtt tcgggagcag gggttcttcg
     1801 tcggctccgg cgtgatcgaa gccggatgca agtctatcgt cggagtacgt ctcaagcaat
     1861 ccggcatgtt ctggaccgtg cgaggcgcca acgccatcat cgctctgcgc tgctgccgct
     1921 tcaatcacag gttcgaagac ttctgggctg agacccgcgc cgcttgattc acaccgctct
     1981 cacttctatg tcgcgcaccc ggtcgagagg tcgaggagga atacctcgca gctctatggt
     2041 ttgtgccaca atttaggcaa cagccttggc attagagcct accgcccgac atcaacga
//
LOCUS       DASRKK010000576         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1125606, whole genome shotgun sequence.
ACCESSION   DASRKK010000576 DASRKK010000000
VERSION     DASRKK010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125606"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1032
                     /locus_tag="VFC15_13395"
     CDS             283..1032
                     /locus_tag="VFC15_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11194.1"
                     /translation="MRSDVVGTSEEGLFPTASVNELSIPHVQMPIMAPELALQAFQSR
                     DEQQNAGLRSYTDDTLVMAELLDTSQRGAFELQRSFVAPRSLSFKPLSFTGDAFVKSN
                     VIVRLLQSEVDYVTKGDPEQEALTATNYKFSYKGDQDLDGRAVHVFQVKPRHKRPGLF
                     KGRIYLDASTGSLVRSEGTVVKSPSFFIRKIEFVQDYADVDHFTLPVHLHSTALARFV
                     GRAVVDVFHRQYQAQPLNASLALNRGLAGQP"
     gene            1041..1853
                     /locus_tag="VFC15_13400"
     CDS             1041..1853
                     /locus_tag="VFC15_13400"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HZM11195.1"
                     /translation="MRTPSSTTDSLSGQEESRTQWVGADSILFATAHRPWPAPMAPWI
                     MTQRWNDLLFLHYALAPEAIRPLVPDVLTLDTYKQRAWVTVTPFWINHLRPPGVPSLP
                     WLSHFSEINVRTYVTYDGKPGVYFFSLDASNLSAVWGARVFYRLPYWQASMKVKGRGG
                     PKLEYVSQRQHGPKPAELRCSYGPASDRFHARLGSIEHFLTERYCLYAFSRKRFYRDD
                     IHHLPWDLQQATFEVEQNTMAQPAGIQLPPQPELVYFARELKVLFWAPERLL"
     gene            1899..>2392
                     /locus_tag="VFC15_13405"
     CDS             1899..>2392
                     /locus_tag="VFC15_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11196.1"
                     /translation="MLKIIAWNIARRVEAWRYLLDTDADIALIQKATEPPVDVVRRID
                     VDPAPWRTAGAGLNRPWRAAVVRLSDRVTVEWLEPKAVDDAHPRELAISRLGTLAAAI
                     LTPTIGESFMVASMYAPWEKPHSKAGSGWIYADASAHRVISDLSSLLGQQEGHRILAA
                     GDLN"
BASE COUNT          468 a          714 c          693 g          517 t
ORIGIN      
        1 tcgtcgttgg gtttggaact taatggcatt gccgtgaatc atatgcggta aggaatctat
       61 gggcaaccgt tccgcatgtg ggaatcgcag aaaaaatcct cggcgaccca cctgcagttt
      121 ccgcgaggac atgccgatag gtacaatgga caatcctctt ttcgcatttc ttaatagcaa
      181 aggacaatgt tgcgagtcac cagtaagtat ctggcaataa ttcttctagc gggtcttccg
      241 gttccagggt tcggtcaagc gtcgcaattc agaccgtcca ctatgcgctc ggacgtggtt
      301 ggcacgagcg aggaaggtct gttccccaca gccagcgtca acgagctgtc cattccgcat
      361 gtccaaatgc cgatcatggc gccggagctt gcgctgcagg cgtttcaaag ccgggacgag
      421 caacagaacg ccggtctgcg ttcgtatacc gacgataccc tggtgatggc ggaactgttg
      481 gacacctcgc agcgaggagc tttcgagttg caacgttctt ttgtggcgcc gcgctccctt
      541 agcttcaaac cgctcagttt caccggcgat gcgttcgtca agagcaacgt gattgtccgg
      601 ctcctgcaat cggaagtgga ctatgtgacc aagggcgacc cggagcagga ggccctcacc
      661 gcaacgaact acaagttttc ctacaaagga gatcaagacc tggacgggcg tgcggtccat
      721 gttttccagg tgaagcctcg ccacaagcgg ccgggactgt tcaaagggcg catttacctg
      781 gatgcttcca ccggaagcct ggtgcgctcg gagggaactg tcgtcaagtc cccgtcattc
      841 ttcattcgca agattgaatt cgtgcaggat tatgcggatg tggaccactt caccctgcca
      901 gtgcatctgc atagcacagc gctggcgcgg tttgtgggcc gggcggtagt tgacgtcttc
      961 caccggcagt accaggcgca gccacttaat gcgtcactcg ccctgaaccg cggcctagcc
     1021 ggccagccgt agccactcgc ttgcgtactc cttcgtccac aaccgacagc ctctccgggc
     1081 aggaagagag ccggacgcag tgggtgggcg ccgattccat tctctttgcc accgcgcatc
     1141 gcccctggcc cgcacccatg gcgccctgga tcatgacgca gcgctggaat gacttgctct
     1201 ttctgcatta cgcattggct ccggaagcga tccgcccgct ggtgccggac gtgctaaccc
     1261 tcgacaccta caagcaacgc gcgtgggtca cggtcacgcc cttctggatc aatcatctgc
     1321 gcccgccggg cgttccatcc ctcccgtggc tgtcacactt ctccgagatc aacgtgcgga
     1381 cctacgtcac ctacgacggc aagccgggcg tgtatttctt cagcctcgat gccagcaact
     1441 tgtcggcggt ctggggtgct cgcgtcttct accgtctgcc ttattggcag gcctccatga
     1501 aggtgaaagg gcgaggcggg ccgaagctcg aatacgtttc gcaacggcaa cacggaccca
     1561 agcccgccga actccgctgc tcctacggcc cggcatcgga caggttccac gcgcggctgg
     1621 gctcgatcga gcacttcctc accgagcgtt attgccttta tgccttcagt cgcaaacggt
     1681 tctaccggga cgacattcac catctgccgt gggacctaca gcaagccacg tttgaagtgg
     1741 agcagaacac catggcccag ccagccggca tccagcttcc tccgcagccc gagcttgtct
     1801 attttgcgcg tgagctgaag gttctatttt gggcgcccga gcgtctgctt taggtcgaaa
     1861 gagctgcgcc tgactggatt tctgggctgc cgaactttat gttgaagata atcgcttgga
     1921 acattgcgcg gcgtgtggag gcgtggcgtt accttcttga taccgatgcc gatatcgctt
     1981 tgattcagaa agcaaccgag ccgcccgttg atgtagtgcg aaggatcgat gtcgatcctg
     2041 ctccgtggcg gacagccggg gctgggctga accgaccgtg gcgggccgct gtcgtcagac
     2101 tctcagatcg cgtcacagtg gaatggctag agccgaaagc agtggatgat gctcacccta
     2161 gagaactggc gattagccga ttaggcacac tggctgccgc gatcctaacg ccgaccattg
     2221 gtgagtcatt catggtcgcg tcgatgtacg ccccgtggga gaagccccac agtaaagcag
     2281 ggagtggctg gatctatgcg gacgcgtcgg ctcaccgggt gatctctgat ctctcgtcgc
     2341 ttcttggcca gcaggagggg catcgtattc tcgcggctgg cgatctcaac at
//
LOCUS       DASRKK010000577        64575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_344184, whole genome shotgun sequence.
ACCESSION   DASRKK010000577 DASRKK010000000
VERSION     DASRKK010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 64575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344184"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFC15_13410"
     CDS             complement(<1..700)
                     /locus_tag="VFC15_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11197.1"
                     /translation="MLKRSLLLNLLIATAATAGYAQDGWKAHQPTLTPQQSGTTQLLI
                     AVSPVNSQIVWAAGTGGTYVVTTDGGSTWKSGVVPGAESLQFRDVQGVSDRVAYLMSI
                     GNNTGDFRIYKTEDAGAHWTIQFKNTTVNAFYDCFAFWTPNRGVTHSDSVNGVFPDIR
                     TTDGKTWQSIAANMPPALSGEASFSSSGTCITTQGRQNAWITTGGSSIARILATRDGG
                     DTWNAYDTPLVSNAN"
     gene            complement(1676..1918)
                     /locus_tag="VFC15_13415"
     CDS             complement(1676..1918)
                     /locus_tag="VFC15_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM11198.1"
                     /translation="MNACCFRIDLWNSCRYLRNGVVTKRKWVDDKELVELLAVSQTLP
                     GLNSTNMAVLVGDNKTNPALLVLAGAIVGAFSFAPK"
     gene            complement(1980..2783)
                     /locus_tag="VFC15_13420"
     CDS             complement(1980..2783)
                     /locus_tag="VFC15_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZM11199.1"
                     /translation="MGLDAGVTPAPGLIYANLSINYSAATLKDAKGNSIPVQGEYSFW
                     AIENILYYVPSFKVLGGTLSFQALLPIGTGSFTLPAFGVNAGGAGYADTWIRPATLGW
                     NFKRASTWVGYAFTAPTGRFTPGSTTNVGSGYWGNNLVSGTTLYLTKNKMTTANLATD
                     WEIHGKKSGTNETPGQAFTMECGVGRLVPLDKTMTKLLELGVIGYDQWQVTADGGTIA
                     DGLSANLIPSTRSMQLGSRPTFFFLRRTPVSSSNLNPSISPIPIRKDAP"
     gene            complement(2911..4620)
                     /locus_tag="VFC15_13425"
     CDS             complement(2911..4620)
                     /locus_tag="VFC15_13425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZM11200.1"
                     /translation="MIYTAKKIITMERGNQEATAVAVSGKQIVVAGSLHEVKKFLGDQ
                     PYRLDETFASKVILPGFIDQHLHPLLGALTLAVEVIAPEDWVVPGKTWKRAANGQEYL
                     TRLKQANAGLQPANEWLFTWGYQQYFHGPIDRRTLDSISSTRAIAVWHRSCHEFYLNS
                     VALETLGITKESIQGKGKWSEQSDWVRGHFYEGGLNLIMAQLLPKLATPERFAFGLKQ
                     MVEMLHQNGVTAFNEPGALVTPQIFKLYQQILGAEDTPFYSFFIADGRGIVDKCGLEG
                     ALSETEKTIALALPGSGKLQFFDKQIKLFADGAIVSQLMQMKAGYTDGHHGEWILAPE
                     ELARRATFYWKAGYQIHTHVNGDLGLEVVLNILEKLMRESPRADSRSVIVHFANSTEE
                     QIERIARLGAIVSANPYYPVGFADKYSEVGLGPKRANSMVRSASVINRCIPYSFHSDL
                     PMGPAAPLYLAWCGVNRITNEGHVAGPEQRISVDAGLRAITIEAAYSWRREDSLGSIA
                     PGKIANFTVLERDPYAVPPEELKDIAIWGTVFEGRLFPIHERARPEARSDEIERGALC
                     AAT"
     gene            complement(4700..6076)
                     /locus_tag="VFC15_13430"
     CDS             complement(4700..6076)
                     /locus_tag="VFC15_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM11201.1"
                     /translation="MSSNGANGTNGNLAFREIPGWDNHNHAGFADAGIATSDGSLRGF
                     FTQWSWAYIEARMPQAEFRAYRTAISLGGDKDVAKTIHQKYPIEKWIDESSEMLQFMT
                     MGVAIRAGCEALYGEYDNVERLNQLMKEARQQPPNYLWDKGCEVGNLKKICTISFGIN
                     RQHYSEKVYKWEPYLDPLFYPFPIKDFPRRGAMAQDFLHGFHLVLPAYMKRYGIGEIP
                     SDFADYREAVGKIVDGMLRDGAVSFKVISLYVRGLDFAAVAEKDAARVFADMSKGNLA
                     ERKTFEDYIARWIFFKLANSSRSPVNFHVSTTHTEPGTTLRMMDLTGLEETLFQDKGL
                     SNQPFIITHAGAIYPGVRQVAALLFHYGNVYADCSEWSYYEYNGGVNAVVTMTEATPG
                     HKLLWGTDGTIPEIFTGSAITSRRMLADALQRRVDTGSLPKKLMIPLAERVLYRNAEK
                     LYGFSVDA"
     gene            6417..7970
                     /locus_tag="VFC15_13435"
     CDS             6417..7970
                     /locus_tag="VFC15_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM11202.1"
                     /translation="MSITATATPPVVSETPTATTPGAVLALAVASLAMSVDPSVHNIA
                     FVGAANSLGMSGDQRSLVASIGTLCIAASILATGSLGDRMGRKKIMLGGLLLATLGSA
                     VTALAQNTAMFTLGRVLAGVGYAASFGLAFALLRAVAPGEAQLSKAVARWLALQTFGV
                     VVLGLLGGYLAGVGWRVAYMLPGIIAVLSFFYCMFAVPEARADDVGPFDTVGLSLVAI
                     GLVSTLYGVSNSASAGWGNTRVLVPLGIGLAVLVAFALYELRLQRPAFPIRLFADPEM
                     AMAGLTGVAFNLGTAVIILQLSLLWQYLYRYSPFKVSFLMMPFSIASILGAACVGSLL
                     ARGVANRILIPAGLISVAAAIAFMGFASASSPYFFFLVPLLFAGVGLMFAQTPAARLF
                     VSKSPPNLVGALGSSRTFFGQFGFALGLALSSSILYGLLGPDLRSRLVAAGAAPAELA
                     QATGIVQSYIHVGSARQFNPQIVYEVLNQARAAYLSSYRIMMLVMSGVIALIAAFIYW
                     RLPSRNSNG"
     gene            8003..8914
                     /locus_tag="VFC15_13440"
     CDS             8003..8914
                     /locus_tag="VFC15_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11203.1"
                     /translation="MPKQNNEVEQAAARTTYSDGHPIDVIQYLESKIILKPDRFTSVQ
                     SFRDFGKSVQRTAKKVGLDFVASPRAKGRPEIREIVFGDTADYRLYNNAFILRRRIPY
                     VDGFPSGDPEIVFKFRHPDERTAAAVDVRPNIAGTYRIKFKMEALPLKNEVGGFRFLY
                     SHNCVFGLSQMHDADKLSMHTLSHVFPSMAVLKTSDEEKISLVSEAIVEEVLLQLGQL
                     HFGKGITADCSVALWRTRGEHQPLVGEFSYQAKFDRKKPIHQKARHLCEQFFVTLQFE
                     VKDWIALGTTKTGMVYRLKGNPPQSHE"
     gene            8972..10657
                     /locus_tag="VFC15_13445"
     CDS             8972..10657
                     /locus_tag="VFC15_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM11204.1"
                     /translation="MTTATLLIADVIYCGDIITMDEKSPTAEAVAVKDGLILAVGTKA
                     DVLKAQGVGTQMIDLGSKTMLPGFIDGHSHFFQAAMIADYANVSAPPVGTAGSIAGII
                     AVLKDHVAKKPLKPGEWLIGYGYDGSAMSDGRDATRDDFDPYFPSNPMVLVHVSGHGC
                     VLNSAGFKAVGIDASTPTPPGGVTVRKPGSNEPAGLLMENSMFPILLKLPKPSPQLLL
                     EHLTIAQQMYASNGYTTVQDAPVEPKVMPLYQKAADEGRLFLDLNGYWESHAFLSSSK
                     PGADYRSPTANHYRLAGVKVIADGSPQGRTAFFSKPYLTGGPGGEKNWCGTPIVPPEH
                     LNAVVKLAYEYDAQVLVHCNGDAAIDMLLDAHQAAGAPEGRRTTVVHSQFVRRDQLDL
                     YVKYGINPSFFTNHAFFWGDVHIENLGQERAFFMSPMKTARWLGLHMTNHSDFLVTPL
                     DPLFILWTAVNRTSRSGQIIGPDERISVLDGLKALTIDGAHQYFEESRKGSITAGKLA
                     DLVILSANPMKVAASTIKDIKAEETIKDGKSVFQRETPAASPPTAEPALVGAR"
     gene            10704..11690
                     /locus_tag="VFC15_13450"
     CDS             10704..11690
                     /locus_tag="VFC15_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZM11205.1"
                     /translation="MTTEVPVIPVTILSGYLGAGKTTLLNSMLRCDHGLRLAVLVNDF
                     GSINIDAALIASHDGETISLTNGCVCCSMADNLAITLLELLSRDTPPDHIVIEASGVA
                     DPRRIASYGAAHPRLRLDGLIVVADAETIRERSVDKYVGELVMRQLLAADLIVLSKMD
                     LIDEPARVCVREFLRETVPHARFLEASHGCVPWDLLLGVGATRSQSKVAIDSQHNHTA
                     PSEGHHAPFSRASFESDRPFDRARLRAAVEALPESVLRAKGVLYVVGEGTRSVALQKV
                     GGRWTLEKGRPWDGGSRRSQLVVIGTFGGLDTAKLAEDFSSALADEPTVESD"
     gene            12342..13052
                     /locus_tag="VFC15_13455"
     CDS             12342..13052
                     /locus_tag="VFC15_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZM11206.1"
                     /translation="MTKDMHQTPRPVLHKHMTQFWSGDLGLTLITISLVILIFVITPL
                     REAGLRGRLFFEPVVVGLMVFGALSVKESYLAQAFVVAVVLVCTTVLMAGRLHPTPFL
                     HQLGSAFVTGTLLLYVRVVLLVMFRVGPVTWSRIQGGVCAYLLLGMAWASAYQLVEQL
                     LPGSFSFVTAPTDMDQLTSKLTYFSFATLTTVGSTIAPMHPIARSLTIAEAIVGQLFP
                     AILIGALVSMAMQSRTKS"
     gene            13123..13500
                     /locus_tag="VFC15_13460"
     CDS             13123..13500
                     /locus_tag="VFC15_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11207.1"
                     /translation="MPSRLEAIEEFIPYTEHPELPRSYPVEAPIWTYFAFARFLALLK
                     FQALHFTRVDRKPARHFSVNHVALGNPVGLISLCGENTERIAIDDRLIGSGRDENLMI
                     VTLKSDKIRPSRILVPRNGGRAA"
     gene            13919..14599
                     /locus_tag="VFC15_13465"
     CDS             13919..14599
                     /locus_tag="VFC15_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HZM11208.1"
                     /translation="MAVVKGAGSFVPPSSKTIEFDGYEWTVAHAISERNGVPNVYDPA
                     NVSVDSDGHLHLRIVRRSDRWTCSEVILPHSFGYGTYRFSVEDISHLEPAVVLTLFTW
                     DDLGAEQNHREMDVEISRWGDPSVKNAQFVLQPYYVPENVVRFSAPPGRLTYSFVWEP
                     AKISFAAAMGAGGTSQRDSVASHVFTSGIPSSGSESVSMNFCAFGYSKVPLKNDAEVI
                     VDNFQYLP"
     gene            14574..15605
                     /locus_tag="VFC15_13470"
     CDS             14574..15605
                     /locus_tag="VFC15_13470"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZM11209.1"
                     /translation="MTIFSIFREGSPGRRQVLSFLLGMAATACLVPSADAVDPNRAFS
                     QYIVDRWTSKNGFPGRQVSVFAQTPDGYLWVGTTNGLFRFDGFNFVSAQELDPTSSSL
                     TGVLGLMTDGYGTLWVRTQGAILRFHNGGFEDVTSELKAGSFITAMSQSIDGGVLLSS
                     LHGEILHGRESFSSIATSARFQTALVIATAQTSDSKVWLGTRADGLFYVSNGRVVPVQ
                     GDLPDRKINSLLPMKDGTLWIGTDAGLAFWNGVALSKKEVPPSLGRVHISALLQDRNG
                     NIWISTPGGLARLGAKGLVLQEKTEPFIGEATTLFEDRDGNLWIGSAQGILLYQSGPG
                     IRRGARWRR"
     gene            complement(15621..17111)
                     /locus_tag="VFC15_13475"
     CDS             complement(15621..17111)
                     /locus_tag="VFC15_13475"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZM11210.1"
                     /translation="MHSVSQAVLRSWSIPPAATFAIALSAVIYLRGWWLLRRAGSPNI
                     PPWRAVTFLLGLLSLWIALASPMDVFNSFVLTAHMLQHMMLMMVVPPLLLLGAPLVPM
                     VRGLPIFAAREFAGPFLNWSVAKRVGTAITNPVFALLLMGVTMFAWHTPVLYELALRS
                     SAWHQTEHACFLFASLIFWWPVVQPWPSRAQWPRWAMVPYLLIADLQNTALCAILAFS
                     DHALYPSYSAVPRLFGFSALEDQVAAGATMWVVGSLAFVVPAIVIAVKCLSRNASQDA
                     IASTRRRETSFFGLAFSRSAEIPLLPRLVKIRWSSKRMEACSFVVLFAAASLCFAGLL
                     AAGANDDDNQTLKFKEISGPFAVAVFSAPGDLDAGPSEFSVLVQDRNTHEVLLGAAVD
                     LAAYPAVDAPGNRVTARASTADSQNKLLQTAEPNLPNEGEWTLNITVSNNSDSGHFSM
                     PLHIVKPEAGVEKPWPYVLISVFSVILAGIYLGRRRFPHGERAVQA"
     gene            complement(17118..18605)
                     /locus_tag="VFC15_13480"
     CDS             complement(17118..18605)
                     /locus_tag="VFC15_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZM11211.1"
                     /translation="MPRLKSIADWFDQRLQIGGAVREAMTHPVPRKSASWWYVFGSAA
                     LTLFILQVFTGIMLALIYVPSAAEAWNSLQFLNHGVTLGWFLRAVHGWGSNFMVAVVL
                     IHLCQVFLFGAYKFPRELTWVVGVFLLLMTLGMAFTGQVLRFDQDAYWGLGIGASITS
                     RVPFLGGYLVHMLLGGPIIAGATLSRFFALHVFVIPGLLIALVSLHLLLVLKLGINEW
                     PMPGRVVRRATYVKEYEELTHRDGVPFVPAAIWKDMFFSAAIIVSVMACAWFLGPFGP
                     SGYPDPTIVQTIPKPDFFFLWLYAMLALLPPSMETPALLIGPVIVIGFLILLPFLSGE
                     GEKSWKRRPIAVVTLLLVAVALGTFTQLATYSPWSPIMNAWSGDPVPVEYLNGRSPLE
                     RQGALVFQVKQCRNCHAIGGSGGKRGPALDRVATNLTRDQLVRQVIQGGGNMPAYGKN
                     LSPAEVTALVFFLETLRPTNQPPARDASQTAIIHEEPGPPAPNNR"
     gene            complement(18593..19144)
                     /locus_tag="VFC15_13485"
     CDS             complement(18593..19144)
                     /locus_tag="VFC15_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZM11212.1"
                     /translation="MSDQDTNAVSRRDLFAKIGMLLNGLAVVILATPVIGYLVSPILG
                     GDRGQYKRWVALGELDEFPEGQTRLAKFQNPITGVSDGETDHIPCWVRRMQGEKFQVF
                     AVNCAHLGCPVRWFAQSGLFMCPCHGGVYYQDGSRASGPPPRGLFEYQHKVEGGKLLI
                     EAGQLPTLSTTASLGARKSGCQG"
     gene            complement(19141..19893)
                     /locus_tag="VFC15_13490"
     CDS             complement(19141..19893)
                     /locus_tag="VFC15_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM11213.1"
                     /translation="MTRRLTLMGVLLAAACGLSACSRLNGKPAPGPEVPRPDSVLDPV
                     VLYSQNCAGCHGAEGRNGPAMDLSDPVYLWIVDDDTLRSTISKGRPGTAMSAFAQKEG
                     GMLTDEQINAIIHGIRERWGKPNVLVNVAVPVYAAKTPGNPQQGQAVYGTYCASCHGE
                     GDKGGPKAGSVTNPSYLSLISDQGLRTIVITGRPDFGAPDWRGNAPGRPMGEQEITDV
                     VAWLAAQRPAKPTQASTANPSSENPSSPGGNH"
     gene            complement(19890..20762)
                     /locus_tag="VFC15_13495"
     CDS             complement(19890..20762)
                     /locus_tag="VFC15_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11214.1"
                     /translation="MRDAMETPDHHVNRNAENVVLPAPTAWPFVMALGVSLILAGLLT
                     NASISVLGTILYLAGAVGWFRQVLPYEHHVEVPVTAEAELVAVAPAPEVMHLPVALQV
                     KRAWLPLKIYPISAGVKGGLAGGVAMALLAMLYGLIFYRSIWYPINLLAGSLYDSPAI
                     PTTEALMHFRFDWFVFALAMHVTMCLLVGLLYGAMLPMLPTRPILLGGVIGPLLWTGL
                     LYRILDFVNPLLDQQISWIWFAASQVAFGVVAGLVVVRQNKVWTSENLPLAMRAGIEA
                     PGIMHERDGEDHKP"
     gene            complement(20769..21365)
                     /locus_tag="VFC15_13500"
     CDS             complement(20769..21365)
                     /locus_tag="VFC15_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HZM11215.1"
                     /translation="MAATTVTLPQSEMPLAHELRGRVGMWCLIAAESAIFSIFVVAYL
                     YYAGKSLNGPTPREVLHVPIFNSICLFASSFTIVMAERAIARGKGRLFGIWWLLTIVL
                     GAIFILGTAREWYHLIYVEGLTISSSLFGTTFYSLVGLHGFHVIVGLIGLSIIMIFAA
                     LGHVKQEHAARIEVFAMYWHFVDAIWVVVLTVVYFVAI"
     gene            complement(21377..23065)
                     /gene="ctaD"
                     /locus_tag="VFC15_13505"
     CDS             complement(21377..23065)
                     /gene="ctaD"
                     /locus_tag="VFC15_13505"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZM11216.1"
                     /translation="MSNAESVLKPMMVSPEREHAKPLFDRVHEWIVTVDHKKLGLMYI
                     VYGILFLVIGGLEATVMRIQLAIPHNHFVSPQVFNQLFTMHGTTMVFLVGMPLLFGFA
                     NYLVPLMIGARDMAFPRLNAFSFWMSAFGGIILYFSFIGGGGLYGAGSAPDVGWFAYA
                     PLTSQAFSPGHSTDYWIIGLLVSGIGSIGTALNIVTTVLCMRCPGMTLGRMPLFAWLN
                     LVTSSMVLIVISPLTAAQIMLLIDRYLGGHFFDTQAGGSAVIWMHFFWIFGHPEVYVL
                     IIPGFAFASEIIPVFSRKPIFGYSIMVAATLAIGFLSLGVWAHHMFTVGMTSYANAFF
                     TASTMAVGVPTGIKIFNWLGTMWGGKIRLTVPMLFCIAFLFQFLIAGLTGIMLAASPF
                     DWQLGNSYFVVAHFHYVLIGGLVFTIFGALYYWYPKVTGRMWNETLGKWHFWLFLIGF
                     HLTFDFMHIPGVLGMPRRIYTYEPGRGWEVWNLIVSLGVIVQIVGILFWVVNFVWSYF
                     RGKLAGNDPWDAWTLEWSTTSPPPEYNFPKIPVVRSRRPLWDLKHPEDPDWKYE"
     gene            complement(23084..24130)
                     /gene="coxB"
                     /locus_tag="VFC15_13510"
     CDS             complement(23084..24130)
                     /gene="coxB"
                     /locus_tag="VFC15_13510"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZM11217.1"
                     /translation="MGLHFRSGVVRRLVSILLLGVTKAAFATAGGDPATPSIFSPAST
                     PADSIYHLSLFVLAICLAIFLVVFGLLVYAVAKFRRRPEDDGREPAQIYGSNQMELAW
                     TVIPVVIVVVLFLATARVIHAVEDARFPPGTTEITAVGHQFWWEFQYPQQGFIAANEL
                     HVPVSDPRNPTPTHITLLSADTDHSFWVPKLAGKTDLIPNRSNSMWIDPHEAGVYVGQ
                     CAQYCGTQHAKMLLRVVVQSREDFDRWVASQQRPVQNAEGVARGRQVFESTACVNCHA
                     VAGTNANGKFGPDLTHLMSRDTIAAGAALNNRENLRQWVKDPDTFKPGALMPAMQLDE
                     QDLDAVTDYLLTLR"
     gene            24582..25274
                     /locus_tag="VFC15_13515"
     CDS             24582..25274
                     /locus_tag="VFC15_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11218.1"
                     /translation="MMLAVAIGQAQQETSVTPTVAGLSIPAELTTTVRAENAHRGDSV
                     EFRTLEAVLIANGLVIPKNAKLLGRIVGAASREGDKPSWLVLLVERAEWKQQTVPLHA
                     FIARQIAITGASQNNYESADSMTPSTVTRRPARENIRSAVEVGADISPMTKLPQDSRT
                     SSRPDPNPNTLMLKDVRIVRDKDGIAYLFSSKANVTLPSGTLFVLQNQAATGVANSGA
                     GDPKAAAPVSPQ"
     gene            25592..26722
                     /locus_tag="VFC15_13520"
     CDS             25592..26722
                     /locus_tag="VFC15_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM11219.1"
                     /translation="MLIVGCEKKEEKPQAGPPEVLVSEVVQQNVPIFQEWVSQLNGPV
                     NADITPKVQGYLLKQDYQNGYFVKKGQLLFELDPRQYQAAVEQAAAQVEIAKAGLSEA
                     DNNVARDTPLAAQNAIPQKQLDTDLSAQAARKAQVEANKANLDNAKLNLAWTKVYSPI
                     YGIAGDAKSQIGDLVGPTTKMATVSQVNPIWAYFNISESSYLGNAAEITKVLRSGKRG
                     NVAVRYIQADGEEYPHGGQITQVNRQVSSGTGTIQLTAEFPNPDAILRPGGFGRVRIQ
                     TSENQNALLVPQAAVIEIQSMYQLIVVTPDNKAVFRPVKVGVRVGANWIITDGLKPGE
                     RVVVEGILKVQQAAAAAPQLVKEGIPVSPKPYVAAASAAGSN"
     gene            26725..29943
                     /locus_tag="VFC15_13525"
     CDS             26725..29943
                     /locus_tag="VFC15_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM11220.1"
                     /translation="MSKFFINRPIVAIVIAILMVLVGVVSILSLPTAQFPNIADPMIQ
                     VRATYPGADAQTIAESVATPIEQQMSGVSGMNYMYSLSASSGGGMTLYVDFQLGTDIN
                     TDQILAQMRSGQANSQLPTEVTQQGVIVQPGTTAPFMLLDLYSPNGTYDSIFLANYAT
                     INLQYALTRLPGVGQVQIFGAGPYAMRIWVNPDKLANLGITVTDITNAVKAQNKVNPA
                     GQVGGEPVPQGQQFTYNVRAPGRLPTAADFDEIVVRAQADGSILRLKDVARVEMGSQY
                     YSFNARLGKAPEEGKPSAPTGPAALIAIYNTPGSNALQSRAAVLKMMEEAKGRFPQGL
                     DYDVALDTTLAVSEGIHEIYKTLGEAIVLVIIVVFIFLQGWRATLIPLLAVPVSLIGT
                     FVVFPLLGFSINTLSLFGLVLAIGLVVDDAIVVVEAVEHHIERGMTPHDATVKAMEEV
                     SGPVIAIALILAAVFIPTAFVPGITGQLYKQFAVTIAISVLFSAFNALTLSPALSAML
                     LRPKKESRGPLGAFFRGFNKVFGSATDGYVNWCRHLIHKAGFAFLLLALVAAAAGFFG
                     KRVPGSFLPDEDQGYLYAGLQLPDASSLQRSSEAARVVEKAILETPGVDKVSSVMGYS
                     MLSGVNATYSSFFFVSLKPWGERKTPETSYNGVKAHLQRALSQIPSGIAFSFPPPAIP
                     GVGTSGGVTFVLEDRSGSGIDFLGKNTQLFMAEARKRPELAGVMTTALFGVPQVGIKV
                     DNAKAMTQQIPLTDLYQTVQTFMGGSLVNFFNRFGLQWQVYVQADGDYRTKAENLGKF
                     YVRNAANEMVPLSTLTSTYPRSGAEFVMRYNLFNCVQINASAAPGYSSGQAIAAMEDV
                     FHKTMPQQMGFDYMGMSYQEVKAAQGVSPATIFGLAFFVVFLIMAAQYESWTLPFSVL
                     LGVPIAVFGAFVTLYLRKLDNNVYAQIGLVMLIGLSAKNAILIVEFAKMEYDKGATLI
                     DAALTGAKLRLRPILMTSFAFLLGCVPLWTATGAGAISRQVLGSVVIGGMLAASFLAI
                     FFIPVSFDVVERFGHWIGGGKKETPPGGTEGQIAGPQGAEHD"
     gene            29936..31324
                     /locus_tag="VFC15_13530"
     CDS             29936..31324
                     /locus_tag="VFC15_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZM11221.1"
                     /translation="MTSIRTSAAIVLLLTVCACKVGPNYKRPAVTVPDQYRGLAPDPS
                     TQPAGQPFADMQWASVFQDEVLQGLIKEALTNNYDIRIAATRVLQANANLGITRADQF
                     PSLGGSAAITNERNALYPGAPTFDTLGLSLNYIVDFWGQYRRATESARATLLATQYGQ
                     SVVQTSLITSVATDYFLLRQYDDELAFSKKTVEADNEILKLNQIKFKGGESAITDVYQ
                     AQALVEQAEAQVITTQQAIEQTENNISILLGRNPGPIARGLGVTEQPHPPDVPAGLPS
                     ALLQRRPDVRRAEENLVAANANVGVAKAAFFPQISLTAAFGAQSTAITSFLQGPATFW
                     AIGGQAVQPIFEGGRIRSSYHLAWAQRDEADLLYKQTVQQAFGEVSNSLVGYSQSRLY
                     RMKIEEQTKTYKQMADLANVRFQGGVTSFLEVQYYEQQYFNSALSLSQAWFAELQYYA
                     QLYQSLGGGWQP"
     gene            complement(31343..31864)
                     /locus_tag="VFC15_13535"
     CDS             complement(31343..31864)
                     /locus_tag="VFC15_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM11222.1"
                     /translation="MDQIPSLIRVFAYLSLLTVGGGMAAFPELKILTVDVHHWLTFPQ
                     LIHMYSLGQMAPGPNMMMIVSIGEWVAGPLGALAVLIAFFGPTAVLALLVGRLWARLE
                     RWPWRDSIQKGLAPVSIGLLVAGCLSMAKGALTGWTTGVIAVSVLMILLVSKINPALL
                     ILCGAVFGVIALR"
     gene            complement(31864..32475)
                     /locus_tag="VFC15_13540"
     CDS             complement(31864..32475)
                     /locus_tag="VFC15_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZM11223.1"
                     /translation="MSDVLVYPAIKEPAAVEVLSAGISVKDIFVEFLLIGSTSFGGGV
                     VAYLRSGLVAKRQWINDKEFVELLAISQSLPGLNATNMAILVGDKLRGVAGSIAAIFA
                     ICLPGAILMYGAGIAYQAHGDRPLATAALRGVAAAAVGLVLATAVQLGKKSLSQYYDL
                     IFVAITILGVNWLHQSVPRVLIAVGLMAIVWYRPRKPATTGTS"
     gene            complement(32551..32889)
                     /locus_tag="VFC15_13545"
     CDS             complement(32551..32889)
                     /locus_tag="VFC15_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11224.1"
                     /translation="MSNPAESGFRATQLSIAPDRRKQDRVVTRLPMRILSVDGNPVFY
                     PAVCTNLGRGGIGFETVERLVVGKVIEFEFVRVIDEAIRYWIKILFRDENRYGGYYVN
                     DDGTDIRPAN"
     gene            33065..34768
                     /locus_tag="VFC15_13550"
     CDS             33065..34768
                     /locus_tag="VFC15_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11225.1"
                     /translation="MRNRQAVRLEQLESMKRSYGPGCAGKCERLLADLRGANFNSAES
                     LIRFHDTLLFLRAFPQSAKVVKLADLLLADVEAQVVKFKASSSAALAFDDESVSGIAG
                     TTVRNTWTYELARWLAQRHAGQIIPEWNIDEQYRNMATILPDVMPLLSDDSFVEADTP
                     YLNWMEAAAGKETVLPWMLQSFEKLPVSRLDRTSLYDALGVNLAWDLGGSFASRTLAR
                     RPVSQFFFHDAPLLQRKHVSLADEMASPSLKLRKLNQSEGGKIIDMARDALAVRYREL
                     HGSTHGDPNYVFETNVGRGVQLFIWGLDAEWRLPLRAYYAGFTLKNGVPVNYFEAIGL
                     FEWIEVGFNTFYAFREGETAWIYSKVLHLLHQLAGATCISVYPYQIGQDNEEAIQSGA
                     FWFYRKLGFRPGRPDLVALAEREEKKIARDPQRRTSPRTLRKLAEGHMFFEFGPGPKG
                     RWDSFSVRALGLVVQRKMATDFGGDAGKMRRATTSKLSKILEVNLDTYTAREQWAFSA
                     FALALALAPGLARWTPAEKRSLLAIIRAKASANETHYLHLLQRHHRLREALLRASAKL
                     S"
     gene            complement(35170..37356)
                     /locus_tag="VFC15_13555"
     CDS             complement(35170..37356)
                     /locus_tag="VFC15_13555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM11226.1"
                     /translation="MRRCVVILLLLVSALSAFAQTKRPITDKDLFRFQWIGDPEASAD
                     GSRVVFVRVTVNEKKDNYDTTLWMVSTSGPDGPIRLTNGKRDSEPRWSPDGKWIAFVR
                     GSAEPPKDGKPLPAEIALLSLAGGEAWVITDLPRSVSGPVWSPDGKHIAFLCDANSDD
                     LAKKQRKDPAGEPEHESDVKVITRAIYRFNGQGYLDPKHHKHIWIIDVPSGSDSKAAA
                     KQLTQSEFDENEPTFTPDGSRIIFHTVRSAEPYYELSTTDIMSVPAVGGAVESLAKVK
                     LGGLLGGLSGMTLSPDGKRIAFIGAVQEPVRSYSQPDLWVLDLQPGAAPKNLTADYDY
                     DVNSGVGGDNGSPRAPGHLSPMWSKDSSHLLDVVAKQGRAILVSVDVSSGKVQELSRG
                     SQAVAQFTSSGDGKVVVMNVSTPTMIDELFVLNQDGSQRQITNINGTLFSDLNLTDPE
                     EIWYTSFDGRKIQAWVQKPPDFNPAKKYPLILNIHGGPHAAYGWIFDHEFQWMAAKGY
                     VVLYPNPRGSTSYGQDFGNIIQYKYPGDDYKDLMTGVDEVLKRGYVDANKLGVTGGSG
                     GGLLTDWTVTQTHRFHAAVAQRDISDWTSWWYTADFTLFQPNWFKAPPFDDPQDYIKR
                     SPITYIKNVDTPMMFILGDVDWRTPPSSGGEQMFRALKFMHKPTVMVHFPGESHELSR
                     SGQPWHRVERLGYIVGWFDKWLMGIPNPELSMPSKETAASAKPATE"
     gene            37537..38313
                     /locus_tag="VFC15_13560"
     CDS             37537..38313
                     /locus_tag="VFC15_13560"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZM11227.1"
                     /translation="MAYKTLDLSGRTAVVIGGTSGIGHALALGFAEAGADVVPTSRRA
                     DLVAQTASEIEGLGRRSLRCPSDVQDRSSLEQVLSQAIEAFGKVDILINCAGRTKRAP
                     TLDFAEADWNTILETNLTGTLRAAQVFGRHMLERGYGRIIHIASVASFVALYEVAAYA
                     ASKAAVASLTKSLALEWGPRGVCVNAIAPGVFPTDLNRSLLEGTARGQEFLSRIPMKR
                     FGDVQELCGAAIFLASEAASYVNGEVLVVDGGFLASGVNQ"
     gene            complement(38374..39636)
                     /locus_tag="VFC15_13565"
     CDS             complement(38374..39636)
                     /locus_tag="VFC15_13565"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZM11228.1"
                     /translation="MVAGRGFTDRSLTSDEIRQIVSTATSELQIDDKRVLIIIPDGTR
                     TMPMPLMFEILQQELGSRAAACDYLVALGTHPLMRDAQLSTLIGQPVVNGVCGASKVV
                     NHRWDLPDTFSELGKIPKEEVVRISGGLLDEAIPVRINRMILDYDRVLICGPVFPHEV
                     VGFSGGNKYLFPGIGGPEIIGWTHWLGALLGSYELIGTLSTPVREIIDMAAELVKVPV
                     ACLSLVVTEEAVSGMYFGAAKEAWRAAADLSAQTHIIWVDQPLQRVLSVMPTLYDDLW
                     TAAKGMYKVEPAVADGGEVIIYAPHISEVSYTHGPDIDKVGYHCRDYFVKQWDKFKDA
                     PRSVLAHSTHLKGKGSYDPGTNTETPRITVTLATGISEERCRKLNLNYLDPKTIDPES
                     WRDREQEGFRLIPRAGEILYRLRSPGES"
     gene            39891..40964
                     /locus_tag="VFC15_13570"
     CDS             39891..40964
                     /locus_tag="VFC15_13570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM11229.1"
                     /translation="MDQKNGGNPSSTNRREFLKAGGAITAGLVAQAAMPAAAATLPAM
                     PSNPATQNAMPTRNLGKTGYKVGIFSLGGQAAIERADNEAVAVPIIEKALDLGVNYID
                     TSSIYGGPQRWSERYVGQVMKKRRGEAFLASKTRERTKAGSLSMLEQSLKLLNTDHLD
                     LWQLHDIGSNSEIEQIFAKGGAMEALLEARDQKTVRFLGITGHFRPEPLIEAIHRYPF
                     DTILMAVNAADPHHFSFSDQLLPLAVDKQMGIIGMKVPARGRILSSWTPPPMEEQKHS
                     WEGMVLAPTPGTLDMKQAMYYSLSLPVSTVIIGCDSVAQLEHNVELAREFTPFSEKQM
                     AGLVQKAEPVSKQALFFRFYDHA"
     gene            complement(40991..42226)
                     /locus_tag="VFC15_13575"
     CDS             complement(40991..42226)
                     /locus_tag="VFC15_13575"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZM11230.1"
                     /translation="MSDSSAPLNSSGRPLTVLCLASYFKGIEFMQECKRQGCRTLLLT
                     SHSLEGADWPRESLDNIFYVPDVDKTWKLQDVLLGISHLSKTEVIDRVVPLDDFDVET
                     AATLREHLRVPGMGDTTARYFRDKLAMRTKARDEGLPVPEFIHILNHQKLNEFMQRVP
                     APWVLKPRSLAGSIGVRKISSADEFWFAVDRLGDQQSFYLLEQYVPGDIFHVDTITYE
                     KELLFSIASKYGRPPMDVSHEGDIFTTRTLPEGSDDEVALRALNQQVLKAFGMVRGVS
                     HSEFIQGRDGKLYFLETAARVGGAHIADLIEAATGINLWAEWAKVEIAGGKAPYHVPP
                     VRHDFAGLLISLARQEHPDTSGYQDPEIVWRMEKKHHVGLILRSTDPARIEQLTHDYA
                     QRFRQDFFASQPPRERPSS"
     gene            42486..45569
                     /locus_tag="VFC15_13580"
     CDS             42486..45569
                     /locus_tag="VFC15_13580"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM11231.1"
                     /translation="MRFALGNKRSWAVAPVVVLFAVFLLTVSGYCQSPAGTLQGEVQD
                     ATHGRVAAATVIVRSLGTSLERQVTTDSRGDFRVPDLQPGNYRVIVNAKGFSEASSDV
                     TVQVSNVRDVLVTLRPAAVQQTVNVKAEPSSITTEELNTTDAVNGGVVTQKDLQDIPL
                     AARSFANIAYMVPGTEPVEPSDPTKARITAVSTGGSSGLNNQLTVDGADNSDDYIGGF
                     LQNFSPDVIREFAVQTSQQNADIGRTTAGSVVISTRSGTNDWHGGFAVYERAAALNAR
                     FPIDNPPPEPKQPFSRQNYVGTIGGPIIKDKLWIFSGFEMVNEHASINYSNNSLTQFN
                     ALSQLAQEGLIPNTTSIAVPPYVSVPFQSYLGMMRIDWAQSKRSQWFVRYALDNSTTN
                     NALVQQATLPSTGATTHSNYQNGAVSNTFVFSPNWLGTFTFGTSYLHGTAARNGYLGF
                     ALAFPFSSTSKTISGFETYGDNQFVTPITAFPVVRNQEKYQFRYDVSASVERHAPKFG
                     VDFIHEPVLSGVFPGQAETTYVLTENPTFYLTNPGQLTAELFCLPPFDGSNCQTTGTA
                     AGNGSFSQNIQRLGLYAMDAWRVTPQLTINYGLRWDTTFGLFQASGVSQLFNPGLQTI
                     RGLQLPLPTGVPQDYRKAFAPRLGISYSPDNVEDLVFRAGIGMYYDDLAQNGWATAFQ
                     AVNTPSAFQPCTAPGDPGCLPGAANGGAGVVIDPNYHTPYALHVTGGVQYQWHKNWTM
                     SADYVLEQGMHGYRRYEYTAGYTLFSPLFPQDTASQMANVPNLSVFKSDNRSSYNAMM
                     LHVQGNVARRFNLTANYVLSSAKTWGCVLGELFDYVNGVCNPLNPFAPGDYGPSGEDV
                     TSRFTLAGIVYVPGGFQVTTLIQAETGRPITLTTPVDVNGFGDPTNDRAVINGVQTTM
                     DQFRGTPYVQVDLRVTRPFKVKERWEILPFIEFFNLFNRNNPGANYVTNISALPTPVD
                     SLYNATHLCPTPACNVPITNPNQLLVPAGALGDFFGPGTTVGIPFAAQLGVKVNF"
     gene            45685..45951
                     /locus_tag="VFC15_13585"
     CDS             45685..45951
                     /locus_tag="VFC15_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11232.1"
                     /translation="MRTLIIIVAGFVVWAICLGSAKLLANASSRSVTVATAVFAVFWF
                     LAAAANMWIGVSRAGYAFRKELPIFLLIFLVPVATAVMVKWKFL"
     gene            46049..46678
                     /locus_tag="VFC15_13590"
     CDS             46049..46678
                     /locus_tag="VFC15_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HZM11233.1"
                     /translation="MTKVIGPRLLSAVDLAGTFLLAVEGATAAITGHLDLLGIMVLSF
                     ATALAGGVIRDLLIGAVPPEAFRDWRYSVVAFAAAGFVFMLHHSVQQVPGTLLMVLDA
                     AGLGLFAVAGTEKSLAYGIHPFIAILMGTITGVGGGMVRDILLAQVPGVLRTEVYATA
                     AFAGSAVLILCRKLKLLPTVSAVAGGIVCFALRVVSVLQHWNLPRVMEP"
     gene            complement(46839..47897)
                     /locus_tag="VFC15_13595"
     CDS             complement(46839..47897)
                     /locus_tag="VFC15_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZM11234.1"
                     /translation="MKGKRSGIPQIAELAGVSIGTVDRALHGRSGINDETRQRVLDIA
                     KKIGYRPNLAARSLSTGRRIRIGVCVPREIRYFYNELWEGIHEEVGRYTDRGIEFVLR
                     PIPELGKGERSAFRKIVDSGVQGVVVAPGNPETMTALIDAAEQSGVRVVCVSTDAPES
                     QRSCIVCVEPRLNGLIAGELMSKFVPPGSKLAILTGMLKTVDHREKAEGFTRSFSDYC
                     AGGSVVTTIEAHEDPEESFNKTRKLLKRIPDLAGIYVNTVNCLPVCRALSAANRAGNV
                     RLIATDLFREMVPYFGDGTIDASMHQRPYRQGQLAVRSLAEHLLHDADLPKANYLNPG
                     IILRSNLHLFRETGGRGA"
     gene            48091..49614
                     /gene="xylB"
                     /locus_tag="VFC15_13600"
     CDS             48091..49614
                     /gene="xylB"
                     /locus_tag="VFC15_13600"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZM11235.1"
                     /translation="MPARLLGIDVGTGGTRAVLVDPQGIVVASRTAEHVAFASPETGW
                     AEQDPRDWCRAACDAIKQVMAGSGTSPNEISAVGFSGQMHGAVLLDAAGEVLRPALIW
                     CDQRTAEEARELTAQLGGDQIIAWTCNAALTNFTLTKLLWVRKHEPKLFNRFRTLLLP
                     KDYVRYRLTGGFGMDMADASGTLLLDVANRRWSTEMCRATGIDIKCLPPLFESPDVCA
                     KISESGAVATGLLQGTPVVAGAGDQAAGAVGLGIVRPGAVHATIGTSGVVFAATDRPA
                     LDPKGRLHTFCHAIPGRWHVMGVTQAAGFSLRWFRDNFGALSQDARDPYERLSEEAAA
                     VPPGCDGVLWAPYLMGERTPHLDPQARAALVGLAANHGRGHIIRAILEGVAFSLRDSF
                     SIFAEMKVPVTDIRLGGGGARSPLWRQIQADVYGHNVEVVEAEEGAAYGAALLAGVGA
                     GTWSSVDEACQQSVRIAKRVEPDPGTSALMNQQYEMYRRIYPALREIYRNSNSSAVT"
     gene            49767..50165
                     /locus_tag="VFC15_13605"
     CDS             49767..50165
                     /locus_tag="VFC15_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZM11236.1"
                     /translation="MPASVPDSHKDLFQKRAFGSFTTLMPDGSPQTTPVWVDFENGKV
                     IVNTALGRQKDKNVRRDPRVAITLIDPDNPYRYLEVRGKVDEITQDGAEKHIDKMAKK
                     YLDKDKYPFAQPGEQRVLIKVTPDKFSTMG"
     gene            complement(50216..50398)
                     /locus_tag="VFC15_13610"
     CDS             complement(50216..50398)
                     /locus_tag="VFC15_13610"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HZM11237.1"
                     /translation="MIEREPIEGIDYYTENGLVVFTAQYLRERGYCCTSGCRHCPYGF
                     ERENSVHTPGERKPSA"
     gene            complement(50395..54756)
                     /locus_tag="VFC15_13615"
     CDS             complement(50395..54756)
                     /locus_tag="VFC15_13615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZM11238.1"
                     /translation="MPLSAIPVSLAWAHPLVAEWFVAKFGSPTEPQEQGWPEILAGRT
                     TLISAPTGSGKTLAAFLICIDRLVRKALDGTLVNRTEVLYVSPLKALGNDIQKNLEGP
                     LSEILALAAERGMLMPEIRTAVRTGDTLAKERRDMLKRPPHILVTTPESLYILLTADK
                     SRAILRDVRTVIVDEIHAVADDKRGAHLALSLERLDLLAGEPTRIGLSATQKPIELVA
                     HFLTGAGRPAPAVVNIGHRRKLDLAVEVPASELGPVASNEMWGEIYDRIAELVQQHRS
                     TLVFVNTRRLAERVALHLSERVGEDLVATHHGSLARKLRLAAERRLKNGEIRVLVATA
                     SLELGIDIGNIDLVCQISSPRAISVALQRIGRAGHWRGAIPKGRLFATTRDDMVECAA
                     AVLAIRSGDLDRIQIPDKPLDILAQQIVAMCSCEDWDEAALFDCVRRAYPYRALTEDE
                     FERILEMLSQGIASQRGRYGAYLHRDLVNRKLRARRGARLAAITSGGAIPETSLYTVV
                     VQPEEIVVGTVDEDFAVESNAGDIMLLGNTSWRIKRVEAKSGRMLVEDARGAPPSVPF
                     WRGEAPSRTAEHSQYVARLREQVSAMLPNTAPLPAPTTDRDGTADAKPRTRLRGLASS
                     PEVQAAVAWLKEECGLDDSGAEQLVEYIVAGRAVLGAVPTQTTIIAERFFDEGGGMQL
                     ILHAPFGGRLNKAWGLALRKRFCRSFNFELQAAATDDGLNIALAEQHSFPLSDVFHYL
                     QVETVQEILEQAALASPIFATRWRWDANRSLALLRFQGGKKVPPYIQRIRSDDLLASV
                     FPDVSACQENIEGDIKIPDHPLIQEVMKDVLTEAMDIDGLRQVLTGIRDGSIRCIAVD
                     TPVPSQFSHEILNANPYAFLDDAPLEERRARAVQMRRMLPEAVLNEIGRLDQTAIALV
                     REEARPDVRDADELHDALQTLVALPEEVADLEWQPVVATWIPFLPELVEGWRVVRTIA
                     GNRRYIVASERAKEFALIFPDAKFEVTPPELPSSADSRDDALLGMIKGWMMHSGPTNA
                     AALGFLLGIPAADVAQALLRMEASGSALRGNFTGHAAGAEPDTAEWCDRRLLARIHHL
                     TVATLRKQVEPVNAAQFMRWLLRWQHLAPNAQASGERGLLEVIRQLQGFEIPANAWEK
                     QVLKPRMNAYDPAVLDQLCLTGAVGWGRVSPHPATLEDSGNGRRRVVPTSVAPITFFV
                     REDSDWMQPRIPDDTENEARVLSESARTVADFLRRRGASFFADIVRGTGKLKAEIETA
                     LWELVAAGLVTADGFENLRSLITPSHRATHGSPKIAKPRHSTGRWSLLYTAEVADRNK
                     AVEAMCQMLLRRYGVVFREVLARESNLPKWRELLITFRRLEDRGEVRGGRFVSGFLGE
                     QFALPEAVESLRAMRNLPATGETLVVSAADPLNLVGFIVPGERVPAISGRYVKFRDGV
                     SVDPEERNSLLREAGAQ"
     gene            54911..55546
                     /locus_tag="VFC15_13620"
     CDS             54911..55546
                     /locus_tag="VFC15_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZM11239.1"
                     /translation="MSEERAKKIKLMIFDVDGVMTDGTIFLFPAPAGTSGSGTQEHRD
                     RMADKVGFGISSSSVVEAKGFNAHDGTGFSLARLGGLKAAIITKRISETVALRARDLK
                     IEHVYQGQQDKAAAFYEILSKEGLQADEVCYVGDDIIDLPVMRLCGLAIAVKNSREEV
                     LRESHYITPHEGGKGAVRDAIEYILRAQGSLQRTIDDYVYSRSQDPAAKVQ"
     gene            55995..57245
                     /locus_tag="VFC15_13625"
     CDS             55995..57245
                     /locus_tag="VFC15_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZM11240.1"
                     /translation="MKNSVAIYSLLLLTVLVLPGTLASAATIAAPKKPHAKSRHVTES
                     PAAQKKMARMKHSSRTTASVNRSSHVVVAKSGKKGKKATYYERFHASSFADDQVDGDI
                     ATGEDPIVRAAAIDALGNMNGTVVSIDPESGRILAMVNQKLALSAGAQPCSTIKVAVA
                     LAALSENVITKDTEIPLGKYYRMDLTTALAHSNNAYFAAVGQKLGFEKVSYYAQQFGL
                     GELAGYNIPGEHRGTFPSTELDAKLGGVGKMCSFGESISLTPLQLGALMAAIANGGSL
                     YYLQHPTTAEEVANFTPKMKRKLDIGPIIPELSDGMSGAVEYGTARMLRSSFVEEPVL
                     GKTGTCSKDGTRFGWFASYANTDYGRIVTVIFLQGGRPTFGPKAAELAGHFYRNLYDH
                     DYFAAKASAIPVRSAGATAIGVVH"
     gene            complement(57314..57892)
                     /locus_tag="VFC15_13630"
     CDS             complement(57314..57892)
                     /locus_tag="VFC15_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11241.1"
                     /translation="MFLFKRVAFVLLLAAAIGAQAQFGIPLNRSDPKQDSENARDLVE
                     RFCRMDYAGARLDPSQWPKVQPLVSWRANPEFPLMMVTVRFDVDPEPTPGHGKYMVTV
                     HYRLLGRYDMAEGYSNESAKTVQDVQFVVSEVNGDWRITDADPNYPHPSRAAVLEWLN
                     KKLADAQDPAAKLTYQHAVQDLQPQKPSPLAK"
     gene            complement(58007..58753)
                     /locus_tag="VFC15_13635"
     CDS             complement(58007..58753)
                     /locus_tag="VFC15_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM11242.1"
                     /translation="MATELVSLTEPQTGAPPEGCIICTEDLWKTYDMGSEQQVHALRG
                     VNVRIQRGEYVAIMGPSGSGKSTLMNLIGCLDTPSKGQYWLNGQLVSELDDDELARIR
                     NKEIGFVFQTFNLLARATSLHNVELPLIYAGVPAQERIERSKQALQSVGMESRMNHKP
                     NELSGGQRQRVAIARALVNTPSIILADEPTGNLDSQTGVEIMALFDRLHQQGNNIVLV
                     THEHDIAEYAHRIIHIKDGRVFSDQKTARA"
     gene            complement(58845..60224)
                     /locus_tag="VFC15_13640"
     CDS             complement(58845..60224)
                     /locus_tag="VFC15_13640"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM11243.1"
                     /translation="MKNWKKVALIAGAVVILLAIVGFTIQQSRKGVVTVQTGKVVRED
                     LTSVVTASGEIKPKVYVNIGANAFGKITHLYVKEGDRVKQGQMLATLENVQSSADVSA
                     NQAALSAAVTDADAAKAAVNTATMDQNQAESEFERAKLDYDRSEALYKAQLIAKADYD
                     TKKAAYGTATAQLGQAKAKLAQAKAQMDSAQRRIPQNAANLRRVTDVLSKTEYTAPFD
                     GVITNLPVREGETVVIGIQNAPGSTLMTIADVSVITAEVKVDETDIVNVRLGETAEVS
                     IDAIPKQRFKGIVTEIGNNAILRSTGVSTAQSTSSSQEAKDFKVVVTIQDPPSNLLPG
                     LSTTARITTASRNSSLAIPIQALTIRQRSELEPEKKSKEAAVQAASLTKGNPKEELQG
                     VFVLKNKKEAVFVPVTTGISGTTDIEVTNGLKEGDEIVTGSYKVLRTLRNGASVKVDN
                     TTGSKKEES"
     gene            60663..62438
                     /locus_tag="VFC15_13645"
     CDS             60663..62438
                     /locus_tag="VFC15_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HZM11244.1"
                     /translation="MKVRIFRPLFSLLVITAFAGSVAVAQSSAPAAADKSASPAASDT
                     KAGTAVAPATTAPADPQADPLKRPVNEKQKKANAKALKQELGSTYKKWLNEDVRWIIT
                     PEEMSAFKQLSNDEERDQFIEQFWLRRDPTPDTEENEFKEEHYRRIAYANEHFAAGKA
                     GWRTDRGRIYIVFGPPDEIDAHPSGGQYDRPMEEGGGETSTYPFETWRYRYIEGLGDK
                     GQQVMIEFVDTCMCGEYHMTIDPNEKDALLHTPGAGLTTYEEMGITNKADRIAGIGSS
                     TLGPMSGLDNTKQFDKLDLYAKLNTAPKVKFKDLEEVVSHKINVNLMPFDIMTDFVKI
                     TGDTVLVPVTIQVKNKDITFVNKDGIQRGTVNIFGRVTGLTGKIAQTFEDTVQVDVPT
                     ELLEKTQEHQSLYWKALPLRPGRYRFDIVVKDVNGDRVGTWSHGVAVPEYNEDKLASS
                     SLILADHMEKVPAKSIGAGNFVIGQTKFAYPHLEPANGSPATFKRDQRLNLWLQIYNL
                     QADAKTNKPSAKVEYEIVNMANNQAVVHSSETTDTMGNLGDQMTLEKSLGLSGFQPGI
                     YRLTVKVDDNISKQQIAPSVRFAVE"
     gene            62523..64220
                     /locus_tag="VFC15_13650"
     CDS             62523..64220
                     /locus_tag="VFC15_13650"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM11245.1"
                     /translation="MTRMLGWLAVAVSLCISAGAGTAPGSISGSVKDSSGVAQMGAVV
                     EMLAVGTGQHVLAFSDADGHFNLAGLAPGNYDLRVTAPSFLPTVREDIALAAGATKIV
                     NITLNTLFEAARMVPSRKRDNEDDDSWKWTLRSTSNRPILRFDDGIPVVVEAGQSDHG
                     LKGSLAFMAGGSNEGYGSASDLGTSFTLEQSIFSTGILAFDGNVGYGAGTGTPDGILR
                     ASYSTSGGENGSPHQLAFTMRRFTGPDLLVHRGSLQALALSSYNTFSLGDSLDLQYGG
                     ELQTIQFMGRANAFRPYGSIDWHVGDNTVIEYRYSTSEPTTRASKGFDSAPADFSESG
                     PRMTLLNGAPLLENAHHHEVSISQRLGDTRLQVAYFRDRIKDPELLGVGDMVTDTGDV
                     LPDVYSGTFSYNGGALNAQGARLVVQRKLTPDMTMTLDYAYGGVLNLEQPGVDWDAIH
                     ADLRQVWRHSAAIKLNGTVPRWNTEWIVSYRWTSGGALTPVDLFNASTGQTDGFFNLF
                     VRQPMPRVHFMPGRMEALIDLRNLLAQGYVPVLGPDGSTVYLVQSARSVRGGVAFTF"
     gene            complement(64295..>64575)
                     /locus_tag="VFC15_13655"
     CDS             complement(64295..>64575)
                     /locus_tag="VFC15_13655"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZM11246.1"
                     /translation="PREDSDGGGSGADGDGDGDQDELFQDAVRIVLEYGKASTSLLQR
                     RLRIGYGRAAHLIDLMERDGIVGAADGPKPREVLKRPDWLSEVEDAMR"
BASE COUNT        13471 a        18795 c        18716 g        13593 t
ORIGIN      
        1 cattggcgtt gctcactagc ggagtgtcat acgcattcca ggtgtcgccg ccatcgcgag
       61 tcgccaggat ccgtgcgatg cttgagccgc cggtggtaat ccaggcgttt tgtcgtcctt
      121 gtgtagtgat gcaggttccg ctcgaggaga acgaagcttc gcctgacaag gcgggtggca
      181 tattggcggc gatcgactgc caggtcttgc catcggtggt gcgaatgtcg ggaaatactc
      241 cgttgaccga atcgctgtgg gtgacgccgc gattcggcgt ccagaaagcg aagcagtcat
      301 agaaggcgtt caccgtcgta ttcttaaatt gaatggtcca gtgcgcgccg gcgtcttccg
      361 tcttgtagat gcgaaaatct ccggtgttgt tcccgatgga catcagataa gcgacccggt
      421 cgctcacgcc ttgcacatcg cggaattgca ggctttcggc tcccggcacg accccagatt
      481 tccaggtcga accgccatcg gtcgtaacca cgtacgtacc gccggttccg gctgcccaga
      541 cgatttggga gttcaccgga ctgacggcaa tcagcagctg cgtcgtgccg ctctgctgtg
      601 gagtgagggt gggttgatga gctttccatc cgtcttgtgc atacccggca gtagcggcgg
      661 tggcaatcaa gagattgagc agcagcgaac gctttaacat agacatcctc cccggactgg
      721 catttcgagc ttggagaagt ataggaacgc cgccgaagcc gggtcaaatc agggacacct
      781 gagtctttaa cggtgcaacg ctcgccgcaa gggaggacag gccattctta gtggaatcgg
      841 cggatcgcga ccgctactcc cgccaaaagc accgacgtac agatcgctcg ctcacgcgtg
      901 cgcctccgcg ccctctcgcc cggcggcctt tgccggagcc agcccgatca ggatggcccc
      961 gactgcctac cggaaagggt caacggctcg ctcgtcagcc atggaaaatg atccatctgg
     1021 acgattagtc ggcaaaccgt tgaactgcca ctcgcgtgca acctcccggg ataccggtat
     1081 ctgggagatg agagcggcct cgacggtcgg acagctgcgt ccgaaaccca acggtcgcgc
     1141 agccggcgcc cgcgtgaaat gggacgattc ccagtggttg aaggcacaaa acctacagcg
     1201 cccggccgca caagccgcgc tctatccgcg ctctgtttgt tggattacca ctgcttttga
     1261 tggtcttggg tgtcatccgc tgacctgcat cgcgttgaaa atacgttgaa ctgatgattc
     1321 gagtcctacc tgaggagcca ttattttcaa taacttacag gcaagcaact cccagaatct
     1381 cctggagttt agctgagaat agctgagaga acagggagtt ggaagatagg cacagcggtt
     1441 ggagtgcctt ttgacggcac cgagcagctg tggaattcaa cccttccact caaggcttcc
     1501 ggtaattcgc cggattctct atttcatgcc agtgttgtgc aatctcccaa actagacaat
     1561 tgaggttcgg ttcttagtaa aggtcgaggt tgagcggagt cggcgtgggc ttcgcgagcg
     1621 cccatgattt tgagcgcccg atcaggacga tcattttagt actttggcgt ttgcactatt
     1681 taggcgcaaa ggagaaggct ccgacgatcg ctccggccag caccagcaat gccggattag
     1741 tcttgttgtc tccgaccaga actgccatat tcgtggagtt cagaccggga agtgtctggc
     1801 tgacggcaag caactcgacc aattccttgt cgtccaccca cttgcgcttg gtgaccacac
     1861 cattgcgcaa gtagcggcaa gaattccaga ggtcgattct gaaacagcac gcattcatgg
     1921 aaccttcgta tcattgcgcg gagcgcgcgg caggccccat gtccacgagc cgccgaagac
     1981 tatggtgcgt ccttgcggat gggaataggc gagatactcg ggttcaaatt tgaagaagaa
     2041 actggcgttc ttcgcaggaa gaagaaagtt ggcctggacc ccaactgcat ggaccgagta
     2101 gaagggatca ggtttgccga aaggccgtct gcaatggtgc ctccgtcagc cgtcacctgc
     2161 cactggtcat acccgattac gcctaactcc aaaagcttgg tcatcgtctt gtcgagcggc
     2221 acgagccgcc cgacgccaca ctccatcgtg aaagcctgcc cgggcgtctc gtttgttccc
     2281 gacttcttgc cgtggatctc ccaatcggtc gcgaggtttg ccgtggtcat cttgttcttt
     2341 gtgagataca gggtcgttcc gctaaccagg ttgttgcccc aatatcccga accaacgttg
     2401 gtcgtggatc ccggagtaaa cctgccggtg ggggcggtga aggcgtaacc gacccaggtg
     2461 cttgccctct taaaattcca ccccagtgtt gcgggccgta tccaagtgtc ggcgtaaccg
     2521 gctccgcccg cgttgacccc gaacgcgggc agcgtaaacg agccagtgcc aatgggcagt
     2581 agggcttgaa aggaaagcgt cccacccaac actttaaagc tcggtacgta gtaaaggatg
     2641 ttttcgatcg cccaaaaact atattcgccc tggactggga tggagtttcc tttagcgtcc
     2701 ttgagagtcg ctgcggagta gttaatcgac aggttggcat agatgaggcc cggagctggc
     2761 gtcaccccgg catcgagacc catttgacct ggtacccact gacctttttg ctgggccgac
     2821 gctccgacaa gggcgacgaa aacggtaccg actagaagtg cgaatctgag tctgctttgc
     2881 accatgagtt ttctacagct cccgtcggcg ctacgtagca gcgcacaacg caccgcgctc
     2941 gatttcatca ctgcgtgctt cgggccgcgc gcgttcatgg ataggaaaca agcgtccctc
     3001 gaagaccgtg ccccagatcg caatatcctt cagctcttcc ggcggcaccg catacgggtc
     3061 ccgctcgagt acagtgaagt tcgcgatctt gcccggcgca atgctgccca gcgagtcttc
     3121 acgcctccat gaataggccg cttcgatggt gatggctctc agcccagcgt caaccgagat
     3181 gcgctgctcg ggcccggcta catgaccctc gttcgtgatc cggttgacgc cgcaccaggc
     3241 cagatagagc ggcgccgcag gccccatggg taaatcagaa tgaaatgaat acgggatgca
     3301 acggttgatg accgaggcac tcctcaccat cgagttggca cgctttggac ctaagcccac
     3361 ctcgctgtac ttatcggcaa atccgacagg ataatacggg ttggcactca cgatcgcgcc
     3421 cagccgcgcg atgcgctcga tttgctcctc ggttgagttc gcaaagtgca caatgacgct
     3481 gcggctgtcg gcgcgcgggg actcgcgcat cagcttttcc agaatgttca ggacaacctc
     3541 aaggcccagg tcgccgttga catgagtgtg gatctgataa ccggctttcc agtaaaacgt
     3601 cgctcgtcgg gcaagttctt ccggcgctag gatccactcg ccgtgatggc cgtcagtata
     3661 gccagctttc atctgcatga gctgcgaaac gatggcgccg tcggcgaaca acttgatttg
     3721 cttgtcgaag aactgcagct tgccgctacc cggcagcgcc agggcgatcg tcttctccgt
     3781 ttccgaaagc gccccttcga ggccgcactt gtccacgatg ccgcgtccgt cagcgatgaa
     3841 gaagctataa aacggcgtat cttcagctcc caggatttgc tggtagagct tgaatatctg
     3901 cggagtaacc agggcgccgg gttcattgaa tgcagtcacg ccattctggt ggagcatctc
     3961 caccatttgc ttaaggccaa acgcaaaccg ctccggtgtc gccagcttcg gcaacagttg
     4021 cgccatgatg aggttgaggc cgccctcata gaaatgaccc ctcacccaat cgctttgttc
     4081 gctccacttg cctttgccct ggattgactc tttcgtgata cccagcgttt ccaacgctac
     4141 cgaattaagg tagaactcgt ggcaggaacg gtgccacacc gctatggctc gcgtgctgct
     4201 gatcgaatcc agcgtccggc gatcgattgg accatgaaaa tactgctggt agccccatgt
     4261 gaagagccac tcattcgcag gctgtaagcc cgcgtttgcc tgtttcaagc gggttaggta
     4321 ttcctgtcca tttgccgccc ttttccacgt tttaccggga acgacccagt cctctggcgc
     4381 gatcacttca accgcgagag tcaaggcgcc gagcaaggga tgcagatgct gatcgatgaa
     4441 tcccggaagg atgaccttgg acgcgaaggt ctcatccagg cggtagggtt gatcgccaag
     4501 aaacttcttt acctcgtgca gcgatccggc tacgacaatc tgcttgccgg agaccgcgac
     4561 agcggttgct tcctggttgc cgcgctccat ggtgatgatc tttttcgcgg tatatatcac
     4621 gagtggagcc tcggcttctt ccggcgcgag gtgacttaaa ggattcatgc acatggattc
     4681 acactacttc tcggtgcctt taggcatcga cgctaaagcc gtaaagcttt tcagcgttcc
     4741 tgtacaaaac cctttctgcc agcggtatca tgagtttttt cggcagagac ccagtgtcca
     4801 cccgacgctg aagagcgtcc gcgagcattc ttctggatgt gatggcagaa ccggtaaaga
     4861 tttccggaat agttccgtcg gtgccccaga gcaacttgtg tcctggggta gcctccgtca
     4921 tcgtgaccac agcgtttact ccgccgttgt attcgtagta agaccattcc gaacaatccg
     4981 cgtacacatt tccgtagtgg aacaacagcg ctgccacctg ccgaaccccg gggtaaatag
     5041 cgccagcgtg ggtaatgatg aacggctggt tgctgagacc tttatcctgg aacagcgtct
     5101 cttctaatcc agtcaagtcc atcatccgca gagtggtgcc aggttctgta tgggtggtgc
     5161 tcacatgaaa gttgactggc gagcgcgagc tgttcgcgag tttgaagaag atccaacggg
     5221 cgatgtagtc ttcgaaagtt tttcgctctg ccagattgcc cttgctcatg tctgcaaata
     5281 cgcgcgctgc gtccttttct gcgactgcgg caaaatcgag cccgcgaacg tagagggaaa
     5341 tgaccttaaa ggaaacggct ccgtccctca gcattccatc gacgatcttg cccacagctt
     5401 cgcggtagtc ggcaaaatca gagggaatct cgccgatgcc atagcgcttc atgtaggcgg
     5461 gcagcaccag atggaatccg tgcagaaaat cctgcgccat cgcgccccgg cgcggaaaat
     5521 ctttgatcgg gaatggataa aagagcggat cgagataagg ctcccacttg tatactttct
     5581 cgctgtaatg ctggcggttg atgccaaaac tgatggtaca gatcttcttt agatttccca
     5641 cctcgcagcc cttgtcccat aaataattgg gcggctgctg acgagcttcc ttcatgagtt
     5701 ggttcagacg ctcgacgttg tcatattcgc catagagcgc ttcgcatcct gcccggatag
     5761 cgacgcccat ggtcatgaat tgcagcatct ccgaagattc gtcaatccac ttctcaatcg
     5821 gatatttctg atggatcgtc ttggctacgt ctttatcgcc cccgagagag attgcggtac
     5881 ggtaggcgcg gaattcggct tgcggcatgc gcgcctcgat gtaagcccac gaccattggg
     5941 taaagaagcc gcgaagtgaa ccatcgctgg tcgctatccc ggcatcggca aaaccagcgt
     6001 ggttatgatt gtcccaaccg ggtatctcgc ggaaggccag attgccattc gtgccgttag
     6061 ctccattgct gctcatgcgg cgactccatg ttctattgat cttcagcgcg cacggcaaca
     6121 tgactgccga ggcgcttgtc aaagagttag cccattaggt aactataaat aagtgaccta
     6181 cgtcaccatt atgtgtgtta ccacgatgca cgaatagaag tggacaacgc gtattattag
     6241 taccgttagt gctagtaggc atcacgcatg cgcgtgactt gcgtcctgtt tcctacttag
     6301 taacagttct atgattactt tctgaaagcg ggatttgctg aacgacatgc ccaatggttt
     6361 cgtcgtcttg gatcaagtcg tccgggagtc cgtcccgcca cagatcggag tgctcgatga
     6421 gcataaccgc taccgcaact ccaccggttg tgtccgaaac gccgacagcg accaccccgg
     6481 gcgcagttct agctctggcc gttgcctctc tggcaatgtc cgtcgatccc tcggtgcaca
     6541 acatcgcctt cgtgggcgca gccaacagtt taggaatgag cggcgaccag cgctccctcg
     6601 tggccagtat cgggacgctg tgcatcgccg ccagcattct cgctaccggc agtctcggcg
     6661 accggatggg ccgcaagaaa atcatgctgg gtggcctttt gctggccacg ctcggctccg
     6721 cggtgactgc gctggcccaa aacacggcga tgttcacctt gggacgcgtt ctcgccgggg
     6781 tcggctatgc tgcttctttc ggattagcgt tcgctttgtt gcgggcagtg gcacccggag
     6841 aggcccagct gtccaaggca gttgcgcgat ggcttgcgct gcaaaccttc ggcgtcgtcg
     6901 ttctcggtct tctcggcggc tatctggcgg gagtcggatg gcgcgtcgcc tatatgcttc
     6961 ccggcattat tgctgtgctg tccttctttt attgcatgtt tgcggtgccg gaagcgcgcg
     7021 ccgacgatgt cggcccgttc gacacagttg gattgtcact ggtcgctatt ggcttggtaa
     7081 gtacgctcta cggcgtgagc aattcggcat cggctgggtg gggtaatacc agggtgctcg
     7141 tcccgctggg gataggtctg gcggtactgg tggcattcgc gctctacgag ttgcgattgc
     7201 agaggccggc ttttcctatc cggctgttcg ccgatccgga gatggcaatg gctggtttga
     7261 ccggtgtcgc cttcaatctt ggtaccgccg tcatcattct gcaactctcc ttgttgtggc
     7321 aataccttta tcgctactcg ccattcaaag tgagttttct gatgatgccg tttagcattg
     7381 cgagtattct cggcgctgcc tgcgtcgggt cgctgctggc gcgtggagtc gctaaccgta
     7441 ttctgattcc cgccggtctc atcagtgtgg cggctgcaat cgctttcatg ggctttgcct
     7501 ccgcttccag tccctatttc ttcttccttg taccgctgct gttcgccggt gtaggtctga
     7561 tgttcgcgca aacgcctgcg gcgcgactgt tcgtcagtaa atctccaccc aatctcgtcg
     7621 gagctctcgg atcgtcgcgg accttctttg gacaattcgg tttcgccctg ggactggcgc
     7681 tgagttcgtc gattctctat ggccttctcg gccccgatct tcgttcgcga ctcgtcgcgg
     7741 ccggcgctgc acccgccgag ttggcgcagg cgaccggaat tgtgcagtcg tacattcacg
     7801 ttggcagcgc ccggcaattc aatccgcaga ttgtctatga ggtcctcaac caggcccgcg
     7861 cggcttactt atccagctac cggatcatga tgctggttat gtcgggtgtt atcgctctga
     7921 tcgcagcgtt catttattgg cggcttccca gtcgcaactc aaatgggtag ggatggaaaa
     7981 acagtttgag agcagaagtt tcgtgccaaa gcagaataat gaggtcgagc aagcggcagc
     8041 caggacgacc tatagcgatg gccatcccat tgatgtcatt cagtacctgg aatccaagat
     8101 catactgaag cccgatcgct tcacttcggt gcagagcttc cgggattttg gcaagtcggt
     8161 gcaacgcacg gcgaaaaagg ttggccttga cttcgtcgcc agcccgcggg ccaagggtag
     8221 acctgagatt cgcgaaatcg tgttcggcga taccgcggac taccggctct ataacaatgc
     8281 ttttattttg cgtcgccgca tcccgtacgt cgacggtttc ccgtccggcg atccggaaat
     8341 tgtcttcaag tttcgtcatc cggacgaacg gaccgcggcc gcagtagatg ttcggcccaa
     8401 catagccggt acgtaccgga tcaagttcaa aatggaagcc ctgccgctga agaacgaagt
     8461 tggcggcttt cggtttctct attcccacaa ctgcgtcttc ggattgagcc agatgcacga
     8521 tgctgacaaa ctctccatgc acacgttgag ccatgttttc ccgtctatgg ccgttcttaa
     8581 gacgtccgat gaagaaaaga tcagcttggt aagcgaagct atcgttgaag aggtcttact
     8641 gcaactggga caactacatt ttggcaaagg gattaccgca gactgcagcg tcgcgctatg
     8701 gcgcacgcgg ggagagcacc agcctctggt gggagagttc tcgtatcagg ccaagtttga
     8761 ccgcaagaaa ccaattcacc agaaggcaag acatttgtgc gaacaattct tcgtcactct
     8821 gcaattcgag gtgaaagatt ggatcgcgct cggcaccaca aaaaccggaa tggtttaccg
     8881 gctgaaaggt aatccaccgc aaagccatga atagcggcaa ccaactcgaa tctgggccgc
     8941 cggcgcgttt tcagcagaag agggagcact tatgacgaca gcaactctat tgatcgccga
     9001 cgtaatctac tgcggcgaca tcatcaccat ggatgagaag tccccgaccg ccgaggcagt
     9061 tgcggtcaaa gacggtttga tcctggccgt tggcacaaag gccgatgtgc tgaaggccca
     9121 gggagtggga acccagatga tcgacttggg tagcaaaacc atgctgcccg ggtttatcga
     9181 cggacatagc cacttctttc aggcggcaat gattgccgat tacgccaatg tttccgcacc
     9241 gccggtcggt acggcgggca gcattgcagg aatcatcgcg gtgctgaaag accatgttgc
     9301 aaagaaaccg ctgaagccgg gcgaatggct gattggctac ggctacgatg gttccgccat
     9361 gtcggacggg cgcgacgcca cacgcgacga ttttgatccg tactttccca gcaacccgat
     9421 ggtgttggtc cacgtgtccg gtcacggttg cgtgctgaac tcggcaggat tcaaggctgt
     9481 cggcatcgac gcgagcacgc caactcctcc tgggggtgtg accgtccgca agcctggcag
     9541 caacgaaccg gccggtctgc tgatggagaa ctcgatgttt ccgattctcc tgaagctccc
     9601 caagccttca ccgcagttgt tgctggaaca cctgacgatt gcgcagcaga tgtatgccag
     9661 caacggttac acaactgtgc aggatgcgcc cgtcgaaccc aaagtcatgc cgctgtatca
     9721 gaaggcagcc gacgagggcc gcctctttct tgacttgaat ggatactggg aatctcacgc
     9781 attcctgagc agctccaagc ccggtgccga ctatcgctcg cctacggcga atcattatcg
     9841 cctcgccggg gtaaaagtca ttgccgacgg ctctccgcag ggccggacgg ccttcttcag
     9901 caagccttac ttgaccggcg gacccggagg cgagaagaac tggtgcggca ctcccatcgt
     9961 ccccccggaa cacctgaatg ctgtcgtgaa gctggcctat gagtacgacg ctcaagtgct
    10021 ggtccactgt aacggcgatg ccgccatcga catgctgctc gatgcacacc aggcggcggg
    10081 cgcgcccgaa ggccgccgga cgaccgtggt ccattcgcag ttcgtgcgac gcgaccagct
    10141 cgacttgtat gtgaaatatg gaatcaaccc gtcgttcttc accaaccatg cgttcttctg
    10201 gggcgacgtt cacatcgaaa acctcggcca ggagcgcgcg ttcttcatga gtccgatgaa
    10261 gactgcgcga tggctggggt tgcacatgac caatcacagc gactttctgg tcaccccgtt
    10321 ggacccgctg ttcattcttt ggacagcggt caatcgcacc tcgcgctcag ggcagataat
    10381 cgggcccgat gagaggatct ccgtgctcga cggcttgaag gcccttacca tcgacggcgc
    10441 acaccagtac ttcgaagaaa gccgcaaagg atcaattacc gcaggcaaac tcgccgatct
    10501 ggtgatccta tccgccaatc caatgaaggt agccgcttcg accatcaagg acatcaaggc
    10561 ggaggagacc atcaaggatg ggaagagcgt cttccagcgt gaaactccgg cagcttcgcc
    10621 cccgacggcg gagccggcgc tggtcggcgc gcgctaggcc acgagccaat gcaaatcgac
    10681 ggagcagaag tcgagagttc cgaatgacga ctgaagtccc ggtgattccc gtcaccatcc
    10741 tcagcggcta cctcggcgcc ggcaagacga cgctgctcaa ctcgatgctg cgctgtgacc
    10801 acggcttgcg tctggccgta ctggtgaatg actttggcag catcaatatc gatgctgccc
    10861 ttattgccag tcatgacggc gagaccatca gccttacgaa tggttgcgtt tgctgcagta
    10921 tggccgacaa cctcgcaatc acgttattgg aactcctcag ccgcgacacg ccgccagacc
    10981 atattgtgat cgaagctagc ggagtcgccg atccacgaag gattgcgtct tatggtgccg
    11041 ctcacccgcg attgcggctg gatggtctga tcgtcgtagc cgacgccgaa accatccgtg
    11101 agcgatcggt cgacaaatat gttggtgagt tggtcatgcg ccagctcctg gcggcagatc
    11161 tgatcgttct cagcaagatg gacttgatcg acgaaccggc gcgggtctgt gttcgggaat
    11221 ttcttcggga gacggtaccg cacgcgagat ttcttgaggc cagccacgga tgcgtcccat
    11281 gggatctgct gctgggcgtc ggcgcgactc ggtcgcagtc gaaggtcgcg atcgacagtc
    11341 agcacaatca caccgcaccg agtgaagggc accatgcacc attcagtcgc gccagcttcg
    11401 agagcgatcg gccattcgat cgcgcacgac tgcgtgcggc agtcgaagct ctacctgagt
    11461 cggtgcttcg tgccaaaggc gttctgtacg tggtaggaga aggcacccgt tccgtcgcgc
    11521 tgcaaaaagt aggcgggcgc tggactctgg aaaaaggacg cccgtgggat ggtggatcac
    11581 gacgtagcca actagtcgtg attggcacgt tcggtggctt agacaccgcg aagctcgcag
    11641 aggacttcag ttctgcatta gcagatgaac ccacggtgga gtcggactga cctgggccgc
    11701 agggtgctgc acgccgaccg cattcgctgc tagcatgctg atcccgtcac ccgcgcctgc
    11761 tcagttcaac atgaacacgg ccaatttcat caagatccca tcaacctaag atgggaacct
    11821 tacccggcca ctgcgctgcg tcagacgttc gttcagggtg cagtggctgc ccttcagttt
    11881 cgcggctgaa tgtgaatcga ggtgcaacga agtgacactg cgtagctcgg gaaaaccgtc
    11941 agctaaatgg tcagctaaac cctcccggcg tagcggtttc gatgccttcg caacccacgc
    12001 gcaactgggc agaacaccca tgggggtcac caactgccct gaggacatcg ggaccaaact
    12061 agttccatca acggtgttgg ccaccacttc gcggacgaca acgaggggtg cgtcctgctt
    12121 tcaagctcgc tcgccgaagc acgtgcgaaa gcgatcacgg cggttatgcg caacgtgaca
    12181 cctggtacga gtgtcgaaat acggttggag cggactgtct ttttcgcgga ccatcttcgc
    12241 accggccttt gcacggaaga atcttgtgga cgattgggcg gcggctcgtc cagaatcgcc
    12301 aaatcggacc tacgccggta atcgctggga ggacgctctt catgacgaag gatatgcatc
    12361 aaactccaag acctgttctt cacaagcata tgacccagtt ctggtctggc gacctgggcc
    12421 ttactctcat aactatctcc ctggtcattc tcatcttcgt aataacgcct cttcgcgagg
    12481 cgggactgcg cggccgcctt tttttcgagc ccgttgtggt cgggctgatg gttttcggcg
    12541 cactgagcgt aaaagagagc taccttgccc aggctttcgt ggtcgccgtc gtcctcgttt
    12601 gtacgacggt tttgatggcc ggtcgcctcc atccgacgcc gtttcttcat cagttgggaa
    12661 gcgctttcgt caccggcacg ctacttctct atgtccgcgt tgtgttgctc gtgatgttcc
    12721 gtgtaggccc ggtaacctgg agccgcattc agggcggagt atgcgcctat cttcttcttg
    12781 gtatggcttg ggcttccgcc tatcagttgg ttgagcaact cctcccaggc tctttctcct
    12841 tcgttaccgc gccaacggat atggaccaac tcacgtcgaa gcttacctat ttcagttttg
    12901 ccacgcttac cacggtcggc agtacgattg ccccaatgca cccgatcgcg cgctcgctca
    12961 ccatcgctga agccatcgtc ggccagcttt ttcccgcgat cctcatcggc gctctcgtgt
    13021 ccatggccat gcagtcccgc accaaatcct gagcccgcag gcatcgcgac acatcgaaaa
    13081 caacgacggc cagcacgata atgactgacc cccacaaggg tagtgccctc tcgtcttgag
    13141 gcgattgagg aattcattcc ctacactgaa catcccgagt tgccgcggag ctaccccgtg
    13201 gaggcgccta tatggacgta cttcgccttc gcacgatttc ttgctttgct taaatttcaa
    13261 gcacttcact ttacgcgggt cgatcgcaaa cccgcccgtc atttttccgt caaccatgta
    13321 gctcttggca accccgttgg cttgatctcc ctgtgcggtg agaatacgga aagaatagcc
    13381 atcgacgacc ggctgatcgg ttccggccgc gacgagaact tgatgattgt gactctaaaa
    13441 agcgataaaa tacgtccatc gcgaatattg gtgccacgca acgggggccg cgccgcatga
    13501 gatggtgggg gttggaaatt ccgaccaatg ctggcagttg gttgactctc tgtgcacttg
    13561 cttcctgcat gctcctcggc ggatgccatt ccaagcaatt gagtaccgcg ccgtcgattg
    13621 agatcgacac ggtacctccg gcgggagagg gagggccggt gaccctcgtg ccgattgcgg
    13681 gtcgcgtgac aggtgcccac cctggcgaca aagttgtgtt gtttgccagg gcggcgaatg
    13741 gaatctggtg ggtgcaacct gtagcgattc agccactcac cccaattgcc gccgattcca
    13801 gttggtacgg ttccgatcca cctgggaacc gattatgcgg ccctgctggt ggaaccggga
    13861 tatcgccccc ccgccaccat gggtgcagct ccctgatagg ggcgacaagg tgctcgcagt
    13921 ggcggttgtc aaaggggctg ggtcgttcgt tccgccgtct tcgaagacca ttgaattcga
    13981 cggctatgaa tggaccgtcg cgcacgccat cagcgaacgc aacggtgtgc ccaacgtcta
    14041 cgatcctgcc aacgtgtcgg tcgactccga cggccacctg caccttcgca tcgttcggag
    14101 gtcggatcgg tggacgtgtt cggaggtaat tctccctcac agttttggct atgggacata
    14161 ccgctttagc gtcgaagaca tttcgcacct cgagccggcg gtagttctga cgctcttcac
    14221 ctgggatgac ctgggagcgg agcaaaacca tcgggaaatg gacgttgaga tcagccgttg
    14281 gggggatcct tccgtcaaga acgcgcaatt tgttctgcaa ccatattatg tcccggagaa
    14341 tgtcgtacgg ttctctgcgc cgccaggaag actgacctat tctttcgtat gggagccggc
    14401 aaagatatcg ttcgcagccg ccatgggcgc aggtgggacc tcgcagcggg attccgtagc
    14461 atcccatgtg tttacttcgg gcattccttc ctccggaagc gagtctgtca gcatgaattt
    14521 ttgcgctttc ggctactcta aggtcccgct caagaacgat gccgaggtta tcgttgacaa
    14581 ttttcagtat cttccgtgaa ggctcgcccg gccgccggca ggttctgagc tttctgctgg
    14641 ggatggcggc gacggcctgc ctcgttccat cagccgatgc cgtggacccc aatagggcct
    14701 tctcgcagta catagttgat cggtggacct cgaagaatgg attccccgga aggcaggtca
    14761 gcgtctttgc tcaaaccccg gacggttatt tatgggtcgg caccaccaat ggtttgttcc
    14821 gttttgacgg attcaatttc gtaagtgccc aggagttgga tcccacgtcg agttccctca
    14881 ctggcgttct cggcctgatg accgacggat atggaacgtt gtgggtccgc acgcaaggcg
    14941 caatcttgcg ttttcataac ggtggcttcg aagacgttac gtctgaactg aaggccggat
    15001 cgttcatcac ggcgatgagc cagtcgatcg acggcggtgt tcttctctcg agtctgcacg
    15061 gcgagatcct gcacggaaga gaaagcttca gcagtatcgc aacatccgca cgctttcaga
    15121 cggcgctggt cattgcgact gcccagacct cggatagcaa ggtttggctg ggcacccggg
    15181 ccgacggtct gttttacgtg agcaatgggc gagtcgtgcc cgtccaaggc gacttgcctg
    15241 atcgaaaaat caactccctt ttgccgatga aggatggcac gctttggatc ggcactgatg
    15301 ccgggctcgc attctggaac ggcgttgcac tgtcaaagaa agaggttcca ccgtctctgg
    15361 gccgcgttca tatctcagca ttgttgcaag accgcaacgg gaacatatgg atcagcacgc
    15421 ctggcggact cgcgcgcctg ggtgccaaag gactggttct gcaggagaaa actgaaccgt
    15481 ttattggcga agcaaccact ctgtttgaag atagagacgg aaatttatgg ataggaagcg
    15541 cgcagggaat tttgctctat cagtctgggc ccggaatccg gcgaggtgct cgatggagac
    15601 gatgaggaga gaaaaatcga tcaagcttga actgcacgtt cgccatgcgg aaagcgacgt
    15661 ctgccgaggt aaattccggc gagaattact gaaaacacgg aaatgagaac gtagggccag
    15721 ggtttctcaa cgcctgcttc gggcttgact atgtgaagcg gcatggagaa gtgaccgctg
    15781 tcagagttat tgctcactgt gatattcaag gtccattcgc cttcgtttgg caagttcggt
    15841 tctgcagttt gcagcagctt gttctgcgaa tccgcagtgc tggctcgcgc ggttacacga
    15901 ttccccggcg catcaactgc cggatatgcg gcgagatcga ccgccgcacc cagcagaacc
    15961 tcgtgagtgt tgcgatcctg cacaagtacg ctgaactcgg acggtccagc gtcgaggtcc
    16021 cccggcgcac tgaaaacggc cactgcaaat ggcccagata tttctttgaa cttgagcgtc
    16081 tgattgtcgt cgtcattcgc tcctgcggcc agcaatccgg cgaagcacag gctcgcggca
    16141 gcaaacaaaa ccacgaacga acacgcctcc atccgtttgc tgctccagcg tatcttcacc
    16201 aaacgtggga gcaacggaat ctccgccgaa cgcgaaaagg ccagtccaaa gaatgatgtc
    16261 tcacgacggc gagttgaggc gatcgcgtct tgcgatgcat ttctcgacaa acacttaaca
    16321 gcaatgacaa tggcgggcac gacgaaggcc agcgatccca ccacccacat ggtggctcca
    16381 gcggcgacct ggtcttcgag agcggagaaa ccaaacagac gcggcacagc ggaatacgac
    16441 ggatagagtg cgtgatcaga gaacgccaga attgcgcaca acgcagtgtt ctgcaggtca
    16501 gcgatcagca ggtagggaac catcgcccaa cgcggccact gggcgcgact aggccacggc
    16561 tgcaccaccg gccaccagaa aatcagcgag gcaaaaagga aacaggcgtg ctccgtttga
    16621 tgccatgccg acgaacgcaa cgccaactcg tacagcaccg gagtatgcca cgcaaacatc
    16681 gtgactccca ttaagagcag cgcgaacact ggatttgtta ttgcggttcc cacgcgcttg
    16741 gcaactgacc aattcaggaa cggcccggca aattcgcggg cagcgaaaat cggcaatccg
    16801 cggaccatgg gaaccagcgg cgcaccgaga agtagcaagg gaggtacaac catcatcagc
    16861 atcatgtgct gcagcatatg ggctgtcagc acgaagctgt tgaacacatc catgggagaa
    16921 gcaagtgcga tccacagaga aagtaggccg agcaaaaacg ttacggcccg ccagggcgga
    16981 atatttggag agccagctcg acggagcagc caccaaccgc gcaaataaat gacggccgac
    17041 aaagcaatcg cgaacgtcgc ggccggagga atcgaccacg aacgcagcac ggcttgagaa
    17101 acagaatgca tgccaggtca gcggttgttc ggggcaggag ggcccggttc ttcgtggatg
    17161 atcgctgttt gcgacgcatc acgggccgga ggctgattcg tgggccgcaa tgtttccagg
    17221 aagaaaacca acgcggtcac ctccgccgga ctcaagttct taccatacgc gggcatgttg
    17281 cctccaccct gaataacctg gcgcaccagc tggtcgcgag taagattggt cgcaactcta
    17341 tcgagcgccg ggccgcgttt gccgccggag cccccaatcg cgtgacagtt gcgacactgt
    17401 ttcacctgga agaccagcgc gccttgacgt tccagcggcg accgcccatt cagatattca
    17461 accggcaccg ggtccccact ccatgcgttc atgatgggac tccacggcga gtaggtcgca
    17521 agctgtgtga acgtgccgag cgccaccgcg accaacagta gcgtgaccac tgcgatcggc
    17581 cgccgcttcc agcttttttc cccttcgccc gagaggaacg gcaacagtat caggaagccg
    17641 atgacgatta ccggcccgat aagcagggcc ggcgtctcca tcgagggtgg cagcaacgcc
    17701 agcatcgcgt acagccacag gaagaagaag tccggcttag gaatagtttg cacaattgtg
    17761 ggatcgggat agccgctagg accgaagggc ccgaggaacc atgcgcacgc cattacgctg
    17821 acgatgatgg cggcggagaa aaacatgtcc ttccaaatcg cggcgggcac gaagggaaca
    17881 ccgtctctat gcgtcaactc ctcgtattcc ttgacgtagg tggcacgccg aaccacgcgg
    17941 ccaggcatcg gccactcatt gataccgagc ttcaacacca gcagcaggtg caacgaaacc
    18001 agcgcgatca gcagtcccgg aatcacgaag acatggagcg caaagaatcg cgagagggtt
    18061 gcgccggcaa taatcggacc gccgagcaac atatggacaa gatatccgcc gagaaaagga
    18121 acacgactgg tgatggatgc accgatgcca aggccccaat aggcatcctg atcgaaacgc
    18181 aacacttggc cggtgaaggc catgccgagc gtcatcagca gcaggaagac gccgaccacc
    18241 caggtgagct cccggggaaa tttgtaggct ccaaacagaa agacctggca caggtggatg
    18301 agcaccaccg ccaccatgaa gttcgagccc caaccgtgta cagctcgcag aaaccatccc
    18361 agagtgactc cgtggttgag aaattgaaga ctgttccacg cttcggcggc cgacggaaca
    18421 tagatcagcg caagcatgat gccggtgaat acttgcagga taaaaagcgt gagcgccgcg
    18481 ctgccgaaga cataccacca gctcgccgat ttgcggggaa cgggatgcgt catcgcttcg
    18541 cggaccgccc cgccgatctg caatcgctgg tcgaaccaat ctgcgatgga cttcagcctt
    18601 ggcatccgct cttcctcgcg ccgaggctcg cggtggtgga caacgtcgga agttgtccgg
    18661 cctcaatcag caatttgccg ccttccactt tatgttggta ctcgaacaat ccgcgcggcg
    18721 gcggtcccga ggcacgcgat ccgtcctggt agtacacgcc accgtgacag ggacacatga
    18781 acaatccgga ttgagcgaac catcgcaccg ggcagccgag atgcgcgcag ttcacggcga
    18841 acacctgaaa tttctcaccc tgcatgcggc ggacccagca gggaatgtgg tccgtttctc
    18901 cgtccgagac accggtgatc ggattctgaa atttcgccag gcgagtttgc ccctcgggaa
    18961 actcgtcgag ttcgcccaat gcgacccaac gcttgtactg tccgcggtcg ccgccgagga
    19021 tgggagatac gaggtagcca attaccggag tggcaagaat cacgaccgcc agtccgttca
    19081 ataacattcc gatcttggca aacagatcgc gccgcgagac tgcgtttgtg tcttggtcgc
    19141 tcaatggttt cccccgggcg aagatggatt ctcagaagaa gggttcgcag tcgaagcctg
    19201 ggttggtttt gcaggtcgct gcgccgcaag ccacgccacc acgtcggtaa tctcctgctc
    19261 gcccattgga cgccccggtg cattcccacg ccagtcgggc gcaccgaaat caggtcgtcc
    19321 ggtgataacg atggttcgca acccctggtc gctgatcaaa ctaaggtacg aaggattggt
    19381 aaccgagccc gccttcggac cgcccttgtc tccctcgcca tgacacgacg cgcaatacgt
    19441 cccgtacacc gcttgcccct gttgcggatt tcccggggtt tttgctgcgt agaccggaac
    19501 cgcgacgttc accagcacgt taggcttgcc ccagcgctcg cggatgccgt gaatgatcgc
    19561 gttgatctgc tcgtccgtca gcattccgcc ttccttttgt gcgaacgccg acatcgcggt
    19621 gccggggcgt cccttggaga ttgttgagcg caaggtgtca tcatcaacga tccacagata
    19681 gaccggatcg gacaaatcca tcgccggacc gttgcggccc tcggctccat gacacccagc
    19741 gcagttctgg ctgtagagaa ctacgggatc cagaacagag tcagggcggg gaacttcggg
    19801 gccgggcgcg ggcttcccat tgaggcgcga gcaagccgaa agcccgcagg ccgcagcaag
    19861 caacactccc atcaacgtga ggcgccgtgt catggtttat ggtcctctcc gtcgcgctca
    19921 tgcataatgc cgggcgcttc aatgcctgcg cgcatggcta gcggcaggtt ctccgacgtc
    19981 cacactttgt tctggcgcac gactacgagt ccggcgacga cgccgaacgc aacctgcgaa
    20041 gccgcgaacc agatccagct aatctgctga tccagcaacg gattcacgaa gtcgagaatg
    20101 cggtagagca acccggtcca aagcagtggc ccaattacgc cgcccagaag aatggggcgg
    20161 gtcggcagca tgggaagcat cgcgccgtag agaagtccaa cgagcagaca catcgtgacg
    20221 tgcatcgcca gcgcgaacac gaaccagtcg aagcggaagt gcatcagcgc ctcggtggtt
    20281 ggtatggccg gcgagtcgta cagactaccg gcgagcaagt taatgggata ccagatgctg
    20341 cgataaaaga tgagcccgta aagcatggcc agtagagcca tggctacgcc gcctgcgagt
    20401 ccgcccttca ctccagcgga gatggggtat atcttcagcg gaagccaggc acgcttaact
    20461 tgcagtgcaa cggggaggtg cattacttcg ggagcaggag ccaccgccac cagctctgcc
    20521 tcagcagtga cgggaacttc gacgtggtgc tcataaggaa gcacctggcg aaaccatccg
    20581 actgcaccgg cgagatagag aatcgttccc aggacgctga tcgaggcatt ggtcagcaag
    20641 ccggcaagaa tcaacgagac gccgagcgcc atcacgaatg gccatgccgt cggcgctggt
    20701 agtacgacgt tctcggcatt cctgttgacg tgatggtccg gcgtttccat agcgtctctc
    20761 atgccacctt agatggcgac aaaatataca accgtgagta ccacgaccca aattgcatcc
    20821 acaaagtgcc agtacatggc gaagacttcg atgcgcgccg cgtgctcctg cttcacgtga
    20881 ccgagcgcgg cgaagatcat gatgatcgaa aggccgatca gtccgacgat cacgtgaaat
    20941 ccatgcaagc cgacgagcga gtagaaagta gttccgaaga ggctggagct gatggtcagc
    21001 ccctccacgt aaatcaggtg ataccattcg cgcgccgttc ccaggataaa gattgcgccg
    21061 agcacgatcg tcagcagcca ccagattccg aacagcctgc ctttgccacg cgcgatcgcc
    21121 cgctcggcca tcacgatagt gaagctgctg gcgaacaggc aaatcgagtt gaagatggga
    21181 acgtgcagta cttcgcgggg cgtcggacca ttcagacttt tgcctgcgta atacagatag
    21241 gcaaccacga agatgctgaa gatcgccgac tccgccgcaa tcaagcacca catgccgacg
    21301 cgtccgcgca attcgtgcgc gagtggcatt tcggattgcg gtagcgtgac cgtcgtagct
    21361 gccattcaac gtctccttac tcgtacttcc agtcaggatc ttcaggatgt ttcaagtccc
    21421 acagcggacg gcggcttcgc accacgggaa tcttcggaaa gttatattcc ggcggtggag
    21481 aagtcgtgct ccactccagc gtccacgcgt cccacggatc gttgcccgcg agctttcccc
    21541 tgaaatacga ccacacaaaa ttgaccaccc agaacaagat gccgacgatc tggacgatca
    21601 ctcccagcga gacgatcagg ttccagactt cccagccccg accgggttcg taggtataaa
    21661 tgcggcgtgg catgcccagt actccgggaa tgtgcatgaa gtcgaaggtg agatgaaagc
    21721 caatgaggaa gagccaaaag tgccacttgc ccagagtctc gttccacatg cggccagtga
    21781 cttttgggta ccagtaataa agcgcgccga aaatggtgaa cactaatccg ccaatcagca
    21841 cgtaatgaaa atgagcgacg acgaaatatg agttgccgag ctgccaatcg aagggcgaag
    21901 ccgccagcat gatgccggtc aatcctgcga tcaggaactg aaagaggaat gcgatgcaga
    21961 acagcatggg tacggtgagc cggatttttc cgccccacat ggttccgagc cagttaaata
    22021 ttttgatgcc ggtcggcaca cctaccgcca tggtcgaggc ggtgaaaaat gcgttggcgt
    22081 aggatgtcat gcccaccgtg aacatgtgat gcgcccacac gccgaggctg aggaagccaa
    22141 tcgccaaggt cgctgccacc atgatggagt agccgaaaat aggtttgcgc gagaacactg
    22201 gaatgatctc cgaagcgaat gcgaaccccg gaatgatcag gacatagacc tcggggtggc
    22261 cgaagatcca gaagaagtgc atccagataa cggccgagcc gccggcttgg gtgtcgaaga
    22321 aatggccgcc gagatagcga tcgatcagca gcatgatctg ggcggccgtc agcggtgaga
    22381 tcacgatcag taccatcgac gacgtgacca gattcagcca cgcgaacagt ggcattcggc
    22441 ccagtgtcat tccaggacag cgcatgcaca aaaccgtagt cacgatgttc agcgcggtgc
    22501 ctatgctgcc aatgccgctg acgagcaatc cgatgatcca gtagtccgtg ctgtgtcccg
    22561 gcgagaaagc ttgcgacgta agcggcgcgt acgcgaacca gcctacatca ggagcgctgc
    22621 ccgcgccgta caggccgccg cctccgatga agctgaagta caagatgatg cccccgaatg
    22681 cgctcatcca gaagctgaag gcgttgagcc gcgggaaggc catatcgcgg gcgccgatca
    22741 tcagcggcac caggtaattc gcaaagccaa acaacagagg catgccgacc aggaaaacca
    22801 tggtggtgcc gtgcatggtg aacaactgat tgaatacctg gggtgagacg aagtggttat
    22861 gcgggatggc caactggata cgcatcaccg tggcttcgag gccgccgatc actaaaaaca
    22921 aaatgccgta cacgatgtac atcaggccga gtttcttgtg gtccacggtg acgatccact
    22981 cgtgcacccg gtcgaacagc ggcttggcat gttcgcgctc gggcgagacc atcatcggct
    23041 tcagaactga ctcggcgttt gacatcactg gttccccata gcttcagcgc aaggtcaaga
    23101 gatagtcggt aactgcgtcc aggtcttgtt cgtccaattg catggcgggc atgagcgcgc
    23161 ctggcttgaa ggtgtccggg tctttgaccc actgccgcaa gttttcgcga ttgttgagcg
    23221 cggcaccggc cgcgatggtg tcgcggctca taaggtgagt gaggtcggga ccgaacttgc
    23281 cgttggcgtt cgtacctgca accgcgtggc agttaacaca tgccgtggac tcaaaaactt
    23341 gacgaccccg ggcaacgcct tccgcgttct gaaccggccg ctgctggctg gcgacccagc
    23401 ggtcaaagtc ctcgcgcgat tgaaccacca cgcgtaaaag cattttggca tgctgagttc
    23461 cgcaatactg cgcgcattgg ccgacgtata caccggcctc atgcggatcg atccacatgc
    23521 tgttggagcg gtttggtatg aggtcagtct tgcctgccag tttggggacc cagaagctgt
    23581 ggtcggtatc ggccgaaagc aacgtgatat gcgtgggagt gggattcctt ggatcgctga
    23641 ccggtacatg caactcattc gcagcgatga aaccttgctg cgggtactga aactcccacc
    23701 agaactggtg gccaaccgcc gtgatctcgg tcgtgccggg cgggaaacgg gcgtcttcaa
    23761 cggcgtgaat cacgcgcgct gtcgcaagga acagcaccac cacaatgacg accggaatga
    23821 cggtccaggc gagttccatc tggttgctgc catagatttg tgcgggctca cggccgtcat
    23881 cctcgggacg ccggcggaat ttcgcgaccg cataaaccag cagaccgaaa accacaagaa
    23941 agatagcgag acagatggcc agcacgaaca gcgacaaatg atagatggag tccgcaggag
    24001 tggaggcggg agaaaatatg ctcggcgtcg caggatcgcc tcccgcggtt gcgaaagcgg
    24061 ctttcgtcac tcccagcaag agaatggaga ctaaccttcg cactacacca cttcggaaat
    24121 gcagccccac aacttaatct ccgagacttc gcggttgtgc acttcttaag attcatgtgg
    24181 gccgcgggac cggaatactc ctgattccgc tcgcggcacg atccggtgag gcgccgaatg
    24241 caacgatgat cgctaggacc aaggttccag ccaattcgca gcagccgatc attgcatcac
    24301 ctctcgcccg gttggaacgt tgccacatcc attccttgag ggtttcgatc ttagcacgca
    24361 gggaggccgg tcaggcaata tgcacgtcgc gtgagtggca ggaatatctg cgacgccggg
    24421 tttgtggcga tgctacctga ttccagcagt tgatcgcagt caaagccacg ccgacgaact
    24481 cgcgaaactc ggctcgattc catctttacg tcagcgccct gccgtactac tctttctgta
    24541 atcggattgg gtggcagatg ttaaggggat gcataccggt cttgatgctg gcggttgcga
    24601 tcggtcaagc gcagcaggag acatccgtca cgccgactgt cgctggactg tcgatccctg
    24661 cggagcttac gaccacggtt cgggcggaga acgcgcatcg gggcgactcc gtcgagttca
    24721 gaaccctgga agcagtcctg attgccaatg gattggtaat acctaagaac gcgaagttgt
    24781 tgggccggat cgtgggcgcg gcatcgcgtg aaggcgacaa gccatcgtgg cttgtgttgc
    24841 tggtggagcg ggccgaatgg aaacagcaga ctgttcccct gcacgcattc atcgcgcgcc
    24901 agatcgcgat tacaggcgcg tctcaaaata attacgaatc agcggactct atgacgccga
    24961 gtacggttac tcgtcgtccc gcccgcgaaa atattcgatc cgcagtcgaa gtcggggccg
    25021 atatctcccc catgaccaag ctgccgcagg actcgaggac gtcttcgcga cccgatccca
    25081 acccgaacac tttaatgctc aaggatgttc ggattgtgcg tgacaaggat ggaatagcgt
    25141 atttgttctc gtcgaaagca aacgtgacac tgccgagcgg caccctgttc gtgctgcaaa
    25201 atcaagccgc tacaggggtg gctaattctg gagcgggcga cccaaaagca gcggcgccgg
    25261 tttccccgca ataacctgta aatcggcggg ccaggagatt tggttgcaat tgctgcccgt
    25321 ccacgaatag cctttactct ggtgaaacca agacattagt gaaggtgcac acgcccaact
    25381 tccgcactgc gaagatatac ttcttcgcac gaataaattt tctcagtaag gccgtttgat
    25441 ggtgcctagg agtgtgtcaa tgcaatccgt gcctgagcat ttctctgtgg aactaaagaa
    25501 agagatcaca agtccatgcc aacaatcggc ttcgcggcaa cgtgggtgtt tcggccgtgc
    25561 gacccttctg ggtttttgtc tgccggtcct attgctgatt gtcggttgcg aaaagaagga
    25621 agagaagcct caagccggtc ctcctgaagt gctggtgtcc gaggtcgtgc agcagaacgt
    25681 ccccatcttt caggagtggg tctcgcagct caacggtccg gtaaatgcag acatcacgcc
    25741 aaaggtacag ggttacctcc tgaagcagga ttaccagaac ggctatttcg tgaagaaagg
    25801 tcagctactc ttcgaactcg accctcggca atatcaggcc gcggtcgaac aagcggcagc
    25861 ccaggtcgag atcgcgaagg cgggtctatc tgaggccgac aacaacgttg cgcgcgatac
    25921 gccgctggcg gcgcagaacg ccatcccgca aaagcagttg gataccgatt tgtccgccca
    25981 ggcagcacgg aaggcgcaag tcgaagccaa caaggcgaac ctggataacg ccaaactcaa
    26041 cctagcctgg accaaggtct attccccgat ttacggcatc gctggcgatg ccaaatcaca
    26101 gattggcgat ttggtgggac ccacaaccaa gatggccact gtctcgcaag taaacccgat
    26161 ctgggcctac ttcaacatca gcgagagtag ctatttggga aacgcggcgg aaattacgaa
    26221 ggttctccgt agcgggaaac ggggcaatgt ggcggtgcgt tacatccagg ctgatggaga
    26281 agagtatccg cacggcgggc aaattaccca agtgaaccga caggtttcct ctggcacggg
    26341 gaccattcaa ctcacggccg aatttccgaa ccccgatgcc atcctgcgcc ccggtggatt
    26401 tggacgagtg cgcatccaga ctagcgaaaa tcagaacgcc cttctggtgc cgcaagcggc
    26461 agtcatcgag attcagtcga tgtaccaact cattgttgtg acgcctgaca acaaagctgt
    26521 gttccgcccg gtaaaggttg gggtcagggt tggcgcgaat tggatcatca cggacggcct
    26581 gaaacctggc gagcgggtcg tggttgaagg catcctgaaa gtgcagcaag ccgctgccgc
    26641 agctccgcaa ttggtgaaag aaggaattcc ggtcagtccc aagccctacg tggccgccgc
    26701 gtcggcggcg gggagcaact gaccatgtcg aaattcttca tcaaccggcc aattgtagcc
    26761 attgtgattg ccattttgat ggtactggtc ggcgtggtct cgattctcag tctgcctacc
    26821 gcgcaattcc ccaatatcgc cgacccaatg atccaggtga gggccaccta tcccggggcc
    26881 gatgcccaaa ccattgcaga gtcggtcgcg acgccgatcg agcagcagat gtcgggtgtc
    26941 agcggcatga attacatgta ctcgctcagc gccagttcgg gcggcggtat gacgctgtat
    27001 gtggatttcc agctcggaac cgatatcaat accgatcaga ttctggcgca aatgcggagt
    27061 gggcaggcca actcacaact gccaaccgag gtgactcagc aaggcgtaat cgtgcaaccg
    27121 ggcacgacag cgccgtttat gctgctcgac ctgtactcgc ccaacggcac ctacgacagc
    27181 attttcctgg cgaactacgc caccatcaat ttgcaatacg cactgacccg tctgccggga
    27241 gttgggcaag tgcagatatt cggcgccgga ccgtacgcca tgcgcatctg ggtcaatccc
    27301 gacaagctgg cgaacctggg aattacggtc actgacatca ccaatgccgt caaagctcag
    27361 aacaaggtca accccgccgg tcaggtaggc ggagagccgg ttcctcaggg acaacagttc
    27421 acttacaacg tgcgggctcc gggccggctg cccacggcgg cggacttcga cgagatcgtg
    27481 gtccgtgccc aggctgacgg ttccattttg cgtctcaagg atgtcgcgcg cgtggaaatg
    27541 ggctcgcagt attactcgtt taacgccaga ttgggaaaag cccctgagga gggcaaaccg
    27601 tcagctccca ctggccccgc ggcgctgatc gcgatttaca acacccccgg cagcaacgcc
    27661 ctccagtcac gcgccgccgt cctcaagatg atggaagaag ccaaggggcg attcccgcag
    27721 ggactcgatt atgacgtcgc cctggacacg acactggcag tgagtgaagg catccacgag
    27781 atttacaaga ccctgggaga ggccatagtc ctcgtcatca tagtcgtgtt tatattcctt
    27841 cagggctggc gtgccacgct gattcctttg cttgcggttc cagtttcact catcggaacg
    27901 tttgtcgtgt tcccgttgct cggcttctcc attaatactc tgtcgttgtt cgggcttgtg
    27961 ctggcgatcg gactggtcgt ggatgacgcc atcgtggtgg tggaggcggt ggagcaccac
    28021 atcgagcgcg ggatgacgcc gcatgacgcg accgtgaagg cgatggagga agtctcaggt
    28081 ccagtgatcg ccatcgccct catcctcgca gccgttttca ttcccacggc cttcgtaccc
    28141 ggcattaccg gacaactcta caagcagttc gccgtcacca ttgcaatctc cgtcctgttt
    28201 tccgccttca atgcgctgac gctgagtcca gcactctctg ccatgctgct gcgtccgaaa
    28261 aaagaatccc gcgggcctct tggggcgttc ttccgtgggt tcaacaaagt gttcggcagc
    28321 gccaccgatg gttacgtgaa ctggtgccga catctgattc acaaggcagg atttgccttc
    28381 ctgctgcttg ccttagtggc cgccgcagcg ggtttcttcg gcaagagggt accgggcagc
    28441 ttcttgcctg acgaggacca gggctatctg tatgccggtt tgcagttgcc ggatgcgtcc
    28501 tcgctgcaac gctcttcgga agccgcccgg gtggtcgaga aggccatcct ggagacgcca
    28561 ggagtcgaca aggtcagctc ggtcatgggc tacagcatgt tgagcggcgt caacgccacc
    28621 tacagttcat tcttcttcgt ctccctcaaa ccatggggag aacgaaagac gcccgagacc
    28681 agttacaacg gagtcaaggc gcacctgcag cgggccctgt cccaaatacc atccggcatt
    28741 gcgttctcat tcccgcctcc agcgattcca ggcgtgggaa cctcaggagg cgtgaccttt
    28801 gtcctcgaag accgatctgg cagcggcatc gattttctcg ggaagaacac acaactgttc
    28861 atggccgagg cccgcaagcg gcccgagctc gcaggggtga tgacgactgc cctgttcggt
    28921 gttccccagg ttgggattaa ggttgataac gccaaagcca tgacccagca aatcccgctg
    28981 actgatcttt accagaccgt acagacgttc atgggcggct cgctggtcaa tttcttcaac
    29041 cgtttcggct tgcaatggca ggtgtacgta caggccgacg gcgactaccg gaccaaggcg
    29101 gagaacctcg ggaagttcta cgtcaggaac gcggccaacg agatggttcc gctgagcacg
    29161 ttgactagca cttatccgcg ctccggtgct gagtttgtca tgcgctacaa cctgttcaac
    29221 tgcgtgcaga tcaatgcctc ggccgcgccg gggtacagtt caggccaggc catcgccgcg
    29281 atggaggacg tcttccacaa gactatgccg cagcagatgg gctttgacta catgggcatg
    29341 tcgtatcagg aggtgaaagc ggctcaaggt gtgtcgccag ccaccatctt cggtctggca
    29401 ttctttgtgg tcttcctgat catggcggct caatacgaga gctggacgct gcctttcagc
    29461 gtgcttctgg gcgtgcctat cgctgtcttc ggtgcgtttg taacgctcta tcttcgcaag
    29521 ctcgacaaca acgtttacgc gcagatcggg ctggttatgc tcatcggact ctcggcgaaa
    29581 aatgccatcc tgatcgttga atttgcaaag atggagtacg acaagggcgc gaccctgatc
    29641 gacgcggcgc tgaccggcgc aaaactccgg ctgcgtccga tcctgatgac gtcgttcgca
    29701 tttctgttgg gctgcgtgcc gttgtggacc gccaccggtg cgggcgccat ttcgcgtcag
    29761 gtgctgggca gcgtcgtgat cggcggcatg ctggcggcct cattcctggc tattttcttc
    29821 atccccgtaa gcttcgacgt ggtcgagcgt ttcggacatt ggattggcgg cggtaagaag
    29881 gagacgccac ccggaggaac agaagggcag atcgcgggcc cgcaaggagc tgaacatgac
    29941 tagcatcaga acatcggctg ccatcgtgtt gctgctcacg gtatgcgcct gcaaggttgg
    30001 gccaaattac aagcggcctg cggttacggt tcctgaccaa taccgcggcc tcgcgcctga
    30061 cccttcgacg caacccgccg ggcagccatt tgcggatatg caatgggcca gtgtttttca
    30121 agatgaggtg ttgcagggcc tgatcaagga agcgctgacc aataactacg acatccgcat
    30181 tgccgccacg cgtgtgctgc aggccaacgc caatttggga atcacgcgcg ccgaccaatt
    30241 cccaagtctc ggcggttcag cggcgattac aaatgagcgc aacgcgctct accccggagc
    30301 acctacgttc gatacgctcg gtctgtcgct taattacatc gtcgatttct ggggacaata
    30361 ccgtcgcgcg acggaatcgg cacgtgccac gctgttggcc acgcaatacg gccaaagcgt
    30421 ggtgcagacc tcgctgatca cctccgtggc caccgactat ttcctgctgc gtcagtacga
    30481 cgatgagctt gcgttctcca aaaagacagt ggaagctgac aacgaaatcc tgaaactgaa
    30541 ccagatcaag ttcaagggcg gcgaaagcgc catcaccgac gtctaccagg cccaggcact
    30601 cgtcgagcaa gctgaagcgc aagtaatcac gacgcaacag gcgatcgagc aaaccgagaa
    30661 caacatcagc atcctgctgg gccggaatcc gggaccaatt gcccgcggcc tcggcgtgac
    30721 tgagcagccc catccgcccg acgttcctgc tggactgcct tccgcgcttc tgcaacgccg
    30781 gccagatgtg cgccgcgcgg aagaaaacct ggttgccgct aatgccaacg tcggcgtggc
    30841 caaagcagca ttcttcccgc agatttctct caccgccgct ttcggtgcgc aaagcacggc
    30901 tatcaccagt tttctgcaag ggcccgcaac cttctgggct atcggtggcc aggccgtgca
    30961 accgatcttc gagggtggcc ggatcaggag cagctaccac ctggcttggg cgcaacgcga
    31021 tgaagccgac ctgctgtaca agcagacggt gcagcaggct ttcggagaag tctccaacag
    31081 cctcgtcggc tacagtcagt ctcgcttgta ccggatgaag attgaagagc agacaaaaac
    31141 ctacaaacag atggcggatc tagccaacgt gcgttttcag ggaggggtca ccagttttct
    31201 ggaagtccag tactacgagc aacagtactt taattccgca ctgagcctct cccaggcgtg
    31261 gtttgccgag ctgcagtact acgcgcagct gtaccagtca ctcggcggag gctggcagcc
    31321 gtaatcgata cgccgatcgt catcacctga gcgcgatcac gccgaacacg gctccgcaca
    31381 agatcaacaa ggctggattg atcttgctta ccagcaggat catcaatacc gacactgcga
    31441 ttacgccagt tgtccacccg gtcagcgcgc ctttggccat cgaaaggcat ccggccacca
    31501 acagcccgat cgagaccggg gccaaccctt tttgaattga atctcgccag ggccatcgct
    31561 ccaacctcgc ccataagcgg cccaccagca gcgccagcac ggcggtcggt ccgaaaaatg
    31621 cgatcaagac cgccaacgct ccaagcggac cggcgaccca ttctccgata gacacgatca
    31681 tcatcatgtt gggtcccgga gccatctggc cgagagagta catgtgaatg agttgcggga
    31741 atgtcaacca atgatggaca tccaccgtga ggatcttcag ttcggggaag gccgccatac
    31801 caccccccac tgtgagcagg gacaagtagg cgaagacgcg aatgagcgaa ggtatctggt
    31861 ccatcacgat gttccggttg ttgctggctt gcgggggcga taccagacga tagccattag
    31921 accaactgcg atgaggaccc gcggcaccga ttgatgaagc caattgaccc ccagaatcgt
    31981 gatggccacg aatatcaggt cgtagtattg gctcagtgat ttcttgccca gttgcactgc
    32041 agttgccagc acgagtccca ccgcagccgc tgccacaccg cgtagcgcag ccgtagccag
    32101 cgggcggtcc ccgtgagcct gatacgcaat cccggctccg tacatcaaga tcgcgcccgg
    32161 cagacatatg gcgaaaatgg ctgcgatgga accggcgact ccacgcagtt tatcgcctac
    32221 caggattgcc atgttggtgg cattcaatcc gggtagcgat tggcttatcg ccaacagttc
    32281 cacgaattcc ttgtcgttga tccactggcg tttggcgacc agtccactac gcagatacgc
    32341 caccacgccg ccaccgaagc tggtcgatcc aatcagtagg aactcaacaa agatgtcctt
    32401 gaccgatatc ccagccgaca gtacttcaac agccgccggt tcttttattg cgggataaac
    32461 aagaacgtcg ctcatgtttc tgctatcgac acagcttacg atgcgtctga atcctgctta
    32521 gccaaaactc ggggctgaac acgaatgtcc ttaattcgct ggacggatgt cggtgccgtc
    32581 atcgttgacg tagtaacccc cgtagcggtt ttcatcgcgg aagaggatct tgatccagta
    32641 gcggatggct tcatcgatga cgcgtacgaa ctcgaactcg atgaccttgc ctaccactaa
    32701 gcgctcgacg gtctcgaagc cgatgcctcc acgacccagg ttcgtgcaca cggctggata
    32761 aaacaccgga ttgccgtcca cgctgagaat ccgcatgggc aggcgagtta ccacgcgatc
    32821 ttgcttcctg cggtcgggag cgatggaaag ttgtgttgct cgaaagccgg attcggccgg
    32881 atttgacatg gttgtacttc tcatcggcag ggcggcatga ttccttgaac cagtctgcaa
    32941 aaacgccaac caggcacgag ggaaaccacg agccacgcgg aaccatcgtg cctaattcgt
    33001 tgcgcgacgc ccgctccggt taaactggcg gacggctcgc caaaatgtcc cggaggcaaa
    33061 cagattgcgg aaccgccagg cagtccgatt ggaacaactt gagtcgatga agcgcagtta
    33121 cggccccggg tgcgccggga agtgtgagcg tctgttggct gatctgcgcg gagcgaactt
    33181 caacagcgcg gagtcgctga tccggttcca tgacacgctg ctctttcttc gcgcgttccc
    33241 ccagagtgca aaggtcgtca agcttgccga cctactactc gctgacgtcg aggcccaggt
    33301 ggtcaagttc aaggcgtcct cgtctgcggc gctcgctttt gatgacgagt cggtctccgg
    33361 cattgccggc accaccgtga ggaatacctg gacctacgag ttggcgcgct ggcttgcgca
    33421 gcgtcatgcg ggccagatca ttcccgagtg gaatatcgat gaacaatatc gaaatatggc
    33481 aacgattctg ccggacgtca tgcccttgct gtcggatgac tcgttcgtcg aggcggatac
    33541 gccttatctg aactggatgg aagctgccgc aggaaaagaa acagtgctgc cgtggatgct
    33601 gcagagcttc gagaagctac ctgtctcgcg actcgatcgc accagcttgt acgacgcctt
    33661 aggggtgaac ctcgcatggg acctcggcgg ctcgtttgcc agccgcaccc tggctcgccg
    33721 tcccgtatct caattcttct tccatgatgc tccgctgctg cagcgcaaac acgtgtcatt
    33781 ggccgacgag atggcttcgc cttcgctcaa gctacgcaag ttgaaccaaa gcgagggcgg
    33841 gaagatcatc gacatggcgc gcgacgctct ggcagttcgc tatcgcgagt tgcacggttc
    33901 cacgcacggc gatcctaatt atgtattcga gaccaatgtt ggcagaggcg tgcagttgtt
    33961 catctggggc ctcgatgccg agtggcgatt gccgttgcgc gcttactatg cggggttcac
    34021 cctgaagaat ggagtgccgg tcaactattt cgaggccatc ggactcttcg aatggatcga
    34081 ggttgggttc aatacgtttt atgcatttcg cgagggtgaa acggcgtgga tctattccaa
    34141 ggtgctgcat ttgttgcatc agcttgcggg tgcgacctgc atttcggtgt atccctacca
    34201 aatcggccag gacaacgaag aggcgattca gtcgggagca ttctggttct atcgcaagct
    34261 cgggttccga cccggccgtc cagatttggt ggccctcgct gagcgggaag agaagaagat
    34321 cgcacgcgac ccccagcgcc ggacatcgcc tcgcacattg cgcaagctgg cggagggaca
    34381 catgttcttc gagtttggtc ctggcccgaa aggacgttgg gattcgtttt ccgtgcgtgc
    34441 tctcgggctg gtggtacagc gaaagatggc aactgatttc ggcggcgatg caggcaagat
    34501 gcggcgcgcg acgacatcca agttgtccaa gattctggag gtcaatcttg atacgtacac
    34561 cgcccgtgaa caatgggctt tcagcgcctt cgcccttgcc cttgccctgg ccccggggtt
    34621 ggcacgatgg acacccgctg aaaaacgctc gctcctggca atcattcgtg ccaaagcaag
    34681 cgccaatgag actcattatc tccacctgct gcaacgccat catcgcctac gggaagcgct
    34741 gctgcgtgcc agcgccaaac tctcctaggc tttggactct ctcgacggtt tcggctgcct
    34801 tcgcagagac cctgttatca cctgccgctg tgtcctgcgt cctatttatc tccagtccac
    34861 tcgtgttatc aatggttcgt gacgcaaagg gagtggtgtg tcttggcctc tcgccttcac
    34921 actacggtct ggctatggag tagttggagc tctttgacaa ccgaaagtct gtttgcggcc
    34981 gtgaccgcgc tcgtcaacat tgcctcaaaa ctagccaaaa gcgacgcttt aactccaatg
    35041 aaatgacatg gatacgtata aatctatttg aatcaacggg tttgcggtat tttttgccta
    35101 acccactgat tctaaaaaac ggtgacaaaa caaaattggg cgacaactgc aaacagtcgc
    35161 acacttttct cattctgtcg cgggtttcgc tgaagcagcc gtctcctttg acggcatgct
    35221 caactcggga ttcggaattc ccatcagcca tttgtcgaac cacccgacta tgtagccgag
    35281 gcgttcgacg cggtgccacg gctgtcccga ccgcgagagt tcgtgcgatt cgccagggaa
    35341 gtgaaccatg acggttggct tgtgcatgaa cttcagtgcg cggaacatct gttctccacc
    35401 gctgctggga ggagtgcgcc aatcgacatc gcccaggatg aacatcatcg gagtgtcgac
    35461 gttcttgatg taggtgatgg gggaacgctt gatgtagtcc tgcggatcgt cgaagggcgg
    35521 cgccttgaac cagttgggct gaaagagcgt gaagtcggcg gtgtaccacc atgaagtcca
    35581 gtccgagata tcacgctgcg cgaccgcagc gtgaaagcga tgagtctggg tgacggtcca
    35641 gtcagtcagc agtccgccac cgctacctcc ggtcacgccc agtttgttgg catcgacgta
    35701 gccgcgcttc aagacttcat ccacgccggt catcaggtcc ttgtaatcgt caccgggata
    35761 tttgtattgg atgatgttgc cgaaatcctg gccgtagctc gtactgccgc gagggttggg
    35821 atacagcacg acgtacccct tggccgccat ccactgaaac tcgtgatcga agatccatcc
    35881 ataggctgcg tgcggaccac cgtgaatatt caaaatcaag ggatatttct ttgcggggtt
    35941 gaagtcgggt ggcttctgca cccaggcctg gatctttcta ccatcgaagc tggtgtacca
    36001 gatctcctct ggatcagtca ggttgaggtc cgagaacaaa gttccgttga tgttcgtgat
    36061 ctggcgttgc gaaccgtcct gattcaacac gaacagctca tcgatcatgg ttggcgtgga
    36121 gacattcatc acgactactt tgccgtcgcc ggacgaggtg aactgcgcca ccgcttggct
    36181 gccgcgtgaa agctcctgca cctttccgct gctcacgtcc acggatacca ggatcgcgcg
    36241 tccctgtttt gcgactacgt cgagcaggtg ggagctatcc ttgctccaca tcggcgacag
    36301 atgaccggga gcccgcggtg agccattgtc gccgcccacg ccgctgttca cgtcgtagtc
    36361 gtagtcggca gtgaggttct tcggggccgc tcccggctgc agatcgagca cccacaaatc
    36421 aggttgggaa taggatcgta ccggctcctg caccgcgccg atgaaggcga tgcgttttcc
    36481 gtccggcgag agcgtcatgc cactgaggcc gcccagcagg ccgccgagtt tcaccttcgc
    36541 cagggattcg actgctcctc ccacagccgg caccgacatt atgtccgtag tggaaagttc
    36601 gtaatacggc tcggcgctgc ggacggtgtg gaagatgatg cggctgccgt cgggagtgaa
    36661 agtaggttcg ttttcgtcga actcgctttg ggtgagctgc tttgcagcgg ccttggaatc
    36721 gctgcccgat gggacgtcaa tgatccagat gtgtttgtgg tgtttcggat cgaggtaccc
    36781 ctgcccgttg aagcggtaga tcgcgcgtgt gattacctta acgtcgcttt cgtgttccgg
    36841 ttcgcccgcg gggtctttcc tttgcttctt cgccagatca tcggaattgg cgtcgcagag
    36901 aaaggcaatg tgtttcccat ccggcgacca caccggaccc gacaccgagc gcggcaggtc
    36961 ggtgataacc caggcttcgc ccccagccag cgaaagcagc gcgatctcgg ccggcagagg
    37021 cttgccgtcc tttgggggct cggcgctccc gcggacaaac gcgatccact tgccgtcggg
    37081 agaccaacgc ggctcggaat cacgctttcc attggtaaga cgaatggggc catcgggacc
    37141 cgaagtggaa accatccaca gcgtggtgtc gtaattgtct ttcttctcgt tgacggtcac
    37201 gcgcacgaaa accacacgag agccgtcagc agatgcctcg ggatcgccaa tccactgaaa
    37261 gcggaagagg tccttgtcgg tgatcgggcg cttggtttgt gcgaaggcgg acagagccga
    37321 gacgagaagc agcaagatga caacacaacg acgcatgagc aacgacctcg aggagagttg
    37381 gaagttagtt ataactgcct gatgcacggt tgtcgaggtt ggccgagggc tgactcgaca
    37441 gaagatgcgg acgatctcca gatgcgacta tgcttcgcac ggattcgata tgattcagtg
    37501 cgactgtgtc tcccaatctg tcagggcgga aaacgaatgg catacaagac tcttgatcta
    37561 tccggaagaa cagcagtggt aattggcgga accagcggca ttggccacgc gctcgcgtta
    37621 gggttcgctg aggcgggcgc cgacgtagtt ccgacgtccc ggcgcgcgga cctggtggcg
    37681 cagaccgcgt ctgaaatcga agggctgggc cgccgctcat tgcgctgccc atcagatgtt
    37741 caggatcgca gcagcctgga acaagttctc tcccaggcga tcgaagcgtt cggtaaagtt
    37801 gacatcctca tcaattgtgc aggacgtacc aagcgcgcgc cgacgctcga cttcgcggaa
    37861 gccgactgga acacaatcct cgaaaccaat ctcaccggga cgcttcgcgc ggcacaggtc
    37921 ttcgggcggc acatgctgga gcgcggctat ggacgcatca ttcacattgc ctccgtcgcg
    37981 agctttgtcg cgctctacga agtggcggcc tatgcggcaa gtaaggctgc tgtggcctcg
    38041 ttgaccaagt cgctggcgct ggagtgggga ccgagaggcg tctgcgtgaa tgccatcgcg
    38101 cctggggtgt tccctacaga cttgaatcgg agcctgttgg aaggcacggc gcgaggtcag
    38161 gagtttcttt cgcgcatccc gatgaagcgt tttggcgatg tgcaggagct ttgtggtgcc
    38221 gccatctttc ttgcttcaga agcggccagc tacgtgaatg gggaagtgct ggtcgtggat
    38281 ggcgggtttc tggcgagcgg ggtgaatcag tagctcgttg atcgatgcca tcgcctgagt
    38341 ggcttcgatt gccgaagcgc tgtcggccgg caactaagat tcgccgggac tgcggagccg
    38401 gtagagaatt tcccccgcgc gtgggattag cctgaaccct tcctgttcgc gatctctcca
    38461 gctttcgggg tcaatggttt tggggtccag ataattcagg ttgagctttc gacagcgttc
    38521 ctcggaaatg ccggtcgcga gtgtcacagt gatgcgcgga gtttcggtgt tggtgccagg
    38581 gtcgtacgat ccctttcctt tcaggtgggt cgagtgtgcc agcacgctgc gcggagcgtc
    38641 tttaaatttg tcccactgtt tcacgaaata gtcgcggcag tggtacccga ccttgtcgat
    38701 atcggggcca tgggtgtagc tgacctcgga aatgtgtgga gcgtaaatga tgacctcgcc
    38761 gccgtcggcg actgccggct cgaccttgta catacccttg gcggcagtcc agagatcgtc
    38821 gtagagggtc ggcatcacgg agagcacgcg ctgcaagggt tgatcgaccc agatgatgtg
    38881 cgtctgcgcg gagagatcgg ctgcggcacg ccacgcctct ttggccgcac cgaagtacat
    38941 tcccgacaca gcctcttccg tgactaccaa cgacaggcag gcgacaggta cttttaccaa
    39001 ctccgcggcc atgtcgatta tttcgcgaac tggcgtcgaa agggtgccga tcaactcgta
    39061 gctgccgagc agcgcgccca gccagtgcgt ccatccgatg atctccgggc cgccgattcc
    39121 aggaaacaga tacttgttgc cgcccgagaa gccgaccact tcatgaggga agaccggacc
    39181 gcagatcagg acccggtcgt agtcgagaat catgcggttg atgcgaacag gaatggcctc
    39241 gtccaacaag ccgccggaga tgcgaaccac ttcctcctta ggaatcttcc ccagctcaga
    39301 aaacgtgtca ggcaggtccc agcggtgatt cacgaccttt gaggccccgc acactccgtt
    39361 gacgacgggt tgacctatga gagtcgagag ttgggcatcg cgcatgaggg gatgcgttcc
    39421 caacgcgacc aggtagtcgc aggccgcggc gcgactgcct aactcctgct gcaggatctc
    39481 gaacatgagc ggcatgggca tcgtgcgggt gccgtcggga atgatgatca gcacgcgctt
    39541 gtcgtcgatc tgcaattcgc tcgtggcggt gctgacgatc tgacggattt cgtccgacgt
    39601 aagcgatcga tcagtgaatc ccctgccggc aaccatttat cttgcgtccc agttcgctcg
    39661 cgatagtttt ggttcagttc tgtattgtcg cattcccgca agaacggtgc aggttcgtgg
    39721 ttgcgcagaa gccgaaaagt ggagagcatg atttcatggg cgcatttctg agcgaggatt
    39781 ttcctgctca tcagcgttag tcgtgccagg tcaccagcca accggttgca aacacgcggc
    39841 gtataatgcg ggtgagtttg cagcgcacag gtagtttctg cgaggtcatt atggatcaga
    39901 agaatggcgg caacccctca tccacgaacc gtcgcgagtt tcttaaggcg ggcggtgcga
    39961 ttaccgctgg actagtcgcg caggcggcga tgccggctgc ggccgcgacg cttccggcga
    40021 tgccttcgaa tccagcaacg cagaacgcca tgcccacgcg caacctgggt aagacgggct
    40081 acaaggttgg tattttcagc ctgggcgggc aggccgccat cgagcgcgcc gacaacgaag
    40141 cggtcgcggt tcccatcatc gagaaggcgc tcgacctcgg cgtgaattat atcgacacct
    40201 cgtcgattta cggtgggccg cagcgctgga gcgaacgcta tgtcgggcaa gtgatgaaga
    40261 agcggcgtgg cgaagcattt cttgccagca agacgcggga gcgcaccaaa gcaggttcac
    40321 tcagcatgct ggagcaatcg ctgaagcttc tcaataccga tcaccttgac ctctggcagt
    40381 tgcacgacat cggttcgaac agcgagattg agcagatctt cgccaagggc ggcgcgatgg
    40441 aagcgttgct cgaggcgcgc gaccagaaga cggtccgctt cctcggcatc acgggccact
    40501 tccgccccga gccactgatc gaggcgatcc atcgctatcc gtttgacacc atcctgatgg
    40561 cggtcaacgc cgccgatccg catcacttca gcttctccga tcaactgctg ccgctggcag
    40621 tggacaagca gatgggtatc atcgggatga aggtacccgc gcgcggccgc atcctctcct
    40681 cgtggacacc gccgccgatg gaagagcaga aacattcctg ggaaggcatg gtgctggcgc
    40741 cgaccccggg cacgctcgat atgaagcagg ctatgtacta ctcgctttcg ctgccggtct
    40801 cgaccgtgat catcggctgc gattcggtgg cgcagttgga gcacaacgtg gaactggccc
    40861 gcgagttcac gccttttagc gagaagcaga tggcgggcct tgtgcaaaaa gcggagccgg
    40921 tatcgaagca ggcgctgttc ttccgcttct acgatcacgc gtaggaactt ccgccgggat
    40981 ttcaccttgg ctagctcgaa ggtctttccc tgggcggttg cgaggcgaag aagtcttgcc
    41041 ggaagcgttg tgcgtagtcg tgcgtcaatt gctcgatgcg cgccggatcg gtggagcgca
    41101 ggatcaaacc cacgtggtgt ttcttctcca ttcgccagac gatttcgggg tcctggtagc
    41161 cggacgtatc cggatgctcc tgcctggcga gagaaatcag tagtccggcg aaatcgtgcc
    41221 ggaccggtgg tacatggtaa ggggcctttc ctcccgcgat ttcaactttc gcccattccg
    41281 cccacagatt aatgccggtc gcggcctcga tgagatcggc gatgtgggcg ccgccaaccc
    41341 gggctgccgt ctccagaaaa tacaactttc catcgcgacc ctgaatgaac tcgctgtgtg
    41401 acaccccgcg caccatcccg aacgccttga gcacctgctg attcagcgcg cgcagggcga
    41461 cttcatcatc cgagccttcc ggcaaagtcc gcgtggtgaa aatgtcgcct tcgtgggaaa
    41521 cgtccatcgg cggccgtccg tacttgctgg caatcgagaa cagcaattcc ttctcgtagg
    41581 taatggtgtc cacgtggaag atatcgccgg gcacatactg ctcgagtaaa tagaaagact
    41641 gctgatctcc cagccggtca acggcgaacc agaactcatc cgcactggag attttcctga
    41701 cgccaatcga tccggctagc gatcgtggct tgagcaccca aggcgcaggt acccgctgca
    41761 tgaactcgtt gagcttctga tgattcaaga tgtgaatgaa ttccggtacc ggaaggcctt
    41821 catcgcgggc cttggtccgc atcgccagct tgtcgcggaa gtagcgggcc gtggtatcgc
    41881 ccataccggg aacgcgcaga tgctcgcgca gtgtcgccgc ggtttccacg tcaaagtcat
    41941 ccaggggaac aacgcggtcg atgacctcgg tcttcgacag gtggctgatt cccagcaaga
    42001 catcctgcag cttccaggtt ttgtccacgt cggggacata gaagatgttg tcgagagact
    42061 ctcgcggcca gtccgcgcct tcgaggctgt gggatgtgag taacaacgtg cggcatccct
    42121 gtcgcttgca ctcctgcatg aactcaatgc ccttgaaata actggcgaga cacaggacag
    42181 tcaggggcct tcccgatgag ttgagtggtg ccgaggaatc cgacaaggca gcctccttcc
    42241 gaggtagatg aataaggtaa cagagaggcc atcgatcgtc tgcgttcacg attttaatga
    42301 ccggcgaaca ccgccgatcg cgtcgaggac gggccgacca ccgcggtgaa atcacataca
    42361 tctacggtta tggtagtagg tcgatgccat tggatagttt cgtacttgcg ggtgtcgctg
    42421 tcgaaactag ccagatagtt tagagggtgg gcgcgcgata actcttccga aaagcaggat
    42481 ctctgatgag atttgcatta ggcaacaagc gttcgtgggc agtcgcgccg gtggtggttc
    42541 ttttcgccgt attcctactg actgtttccg gttattgcca aagtccagcg ggcacattgc
    42601 agggcgaagt gcaggatgcc acgcatggcc gggttgcggc tgctaccgta atagttcgtt
    42661 ctcttggcac gtctctggaa cgacaggtga cgacggacag ccggggcgat tttcgggtcc
    42721 ctgatttgca gccgggaaac taccgcgtca tcgtgaatgc gaaaggattc tccgaagcca
    42781 gctccgatgt aacggtgcag gtcagcaacg tgagggacgt tctggtcacg ctgcggcctg
    42841 cagccgttca gcagacggtg aacgtgaagg ccgagccatc ttccattacc accgaagagc
    42901 tcaataccac cgacgcggtg aacgggggcg tagtcacgca gaaagatttg caggacattc
    42961 ccctggccgc ccgcagcttt gccaatatcg cctacatggt cccgggtacg gaaccggtcg
    43021 agccttccga tcctaccaag gcccgcatca ctgcggtgtc aaccggcgga agctccggtt
    43081 tgaacaatca gctcacggtc gatggcgccg acaactcgga cgactatatc ggcggtttcc
    43141 tgcagaattt ttcgcccgat gtgatccggg aattcgcggt gcagacctcg cagcagaatg
    43201 ctgatatcgg gcgcacaacg gcgggttcgg tcgtcatcag caccagaagc ggcaccaacg
    43261 actggcatgg cggatttgcc gtgtatgagc gcgcagccgc actgaatgca cgtttcccca
    43321 tcgacaaccc gccgccggaa cccaaacagc cgttctcccg ccagaactac gtcgggacga
    43381 ttggcggtcc catcatcaaa gacaagctgt ggattttttc cgggtttgag atggtgaacg
    43441 agcacgccag catcaattac agcaacaata gccttaccca attcaacgcg ctgtcgcaat
    43501 tggctcagga ggggttgatt ccgaacacga cttccatcgc tgtgcctccc tatgtgagtg
    43561 tgccgtttca gtcatatctc ggtatgatgc gcatcgactg ggcgcaatcg aagcgttcgc
    43621 agtggttcgt gcgctatgcc ctcgacaatt ccaccaccaa caacgcgctg gtacagcagg
    43681 ccacgttgcc ttccacgggt gcgaccacgc actccaatta tcagaatggc gccgtcagca
    43741 atacatttgt cttcagcccc aactggttgg ggaccttcac ctttggcacc agttatctgc
    43801 acggcacagc agcgcgtaat ggatatctcg gttttgcgct ggcgtttcct ttcagttcca
    43861 catccaagac gatctcgggt tttgaaacct atggcgacaa tcagttcgtc actccgatta
    43921 cggcgttccc tgtggtccgc aaccaggaga aatatcagtt tcgttacgat gtaagcgcgt
    43981 cggtggagag gcatgctccc aaattcggtg tggacttcat ccacgaaccc gttctgagcg
    44041 gggtgtttcc aggtcaggca gaaacgacat atgttctgac cgagaacccg actttctatc
    44101 taaccaatcc aggacaactg acggcggagt tgttctgcct gccgccattc gacggcagca
    44161 actgccagac gaccggcacg gcggccggaa acggcagttt ctcgcagaat attcagcggc
    44221 tcggtctgta cgccatggat gcgtggcgag tgacgccgca gttgaccatc aactacggcc
    44281 tgcgctggga caccacattt ggactgttcc aggcatcggg tgtaagccag ttattcaacc
    44341 ctgggttgca aaccatccga gggttgcaac tgcccctgcc cacaggtgtg ccccaggatt
    44401 accgcaaagc cttcgctccg cgcctgggca tctcatattc tcccgacaat gttgaggact
    44461 tagtcttccg cgccggaatc ggcatgtact acgacgactt ggctcagaac ggatgggcga
    44521 ccgccttcca agccgtaaac acgccgtcgg catttcagcc ttgcaccgcg ccgggtgacc
    44581 ccggatgcct gccgggcgcg gccaacggtg gcgccggagt ggtgatcgac cctaattacc
    44641 acacgccgta tgcgctgcac gtcactggcg gcgtgcagta tcagtggcac aagaactgga
    44701 caatgagtgc cgactacgtc ctcgaacagg gtatgcacgg ctatcgtcgg tacgagtaca
    44761 ccgcaggcta caccctgttc tcgccgctgt tcccgcaaga caccgcaagc cagatggcca
    44821 acgtgcccaa cctgtccgtc ttcaagtccg acaaccgctc cagctacaac gcgatgatgc
    44881 tgcacgtgca gggaaacgtc gctcgccggt tcaacctgac cgcgaactac gtcctgtcga
    44941 gcgccaagac ctggggttgc gtgctgggcg agctcttcga ttacgtgaac ggcgtttgca
    45001 atcctctgaa tcccttcgcg cccggagact atggcccatc gggagaagat gtcactagcc
    45061 gctttacgct cgcggggatc gtttatgtcc ccggcggctt ccaggtgacc acactgattc
    45121 aggctgagac cgggcgtccg atcacgttaa ccactccggt cgacgtgaat ggatttggcg
    45181 accctacgaa tgatcgcgcc gtgatcaacg gtgtccagac gacgatggac caatttcggg
    45241 gaactccgta cgtgcaggtt gatctacgcg tcacgcgacc gttcaaagtc aaggagcgct
    45301 gggagatttt gccatttatc gagttcttca atctgttcaa ccgcaacaat ccgggcgcca
    45361 actatgtgac caacatctcc gccctgccga cgccggtgga cagcctgtac aacgccacac
    45421 atttgtgccc gactcccgcc tgcaacgtgc ccatcaccaa tcccaatcaa ctgctggtgc
    45481 ctgccggcgc gcttggcgat ttcttcgggc cgggaaccac ggtggggatt ccgtttgccg
    45541 cacagctggg agtgaaggta aatttctaag tctcggcgcg aacgcggaag gctaactcgc
    45601 tgaggtggat gcgggcctct gccgatacgc agaataggcc tcgccactgg tccgcttctg
    45661 cgcccaagcg gcgggaaggg aaacatgcgc accctgatca tcatcgtggc gggcttcgtt
    45721 gtttgggcga tctgtttagg tagtgcgaag ttgctcgcga atgccagcag caggtcagtt
    45781 acggtcgcta cagcggtatt cgccgtgttt tggttcctgg ctgccgcagc gaacatgtgg
    45841 atcggagtgt cgcgggccgg ctatgcattc aggaaagagt tgccgatctt tctgctgatc
    45901 tttctggtgc cggtcgcgac cgccgtcatg gtcaagtgga agtttctttg agacaagcga
    45961 ttcaaccgcg agacgggtat ggatttgcgc cacgtcttgg gcagatcttc gtgattttac
    46021 ggtccggtcg gtgatgaaag ctcccaggtt gacgaaggta atcggtccca ggctgctctc
    46081 cgcagtcgat ctggcgggga cgtttcttct tgccgttgaa ggggcgaccg ccgcgataac
    46141 cggccacctc gatttactgg gtatcatggt gttgtcgttc gccaccgcgt tggctggtgg
    46201 tgtgattcgc gatttgctaa taggtgcagt cccgccggag gccttccgcg attggcgata
    46261 ttctgttgtg gctttcgccg ccgccggttt cgtgttcatg ctgcatcatt cggtgcagca
    46321 agttcccggc accctgctca tggttctgga cgcagccggc ctcggacttt ttgctgtcgc
    46381 tggaacggaa aagtcacttg cctatggtat ccatccgttc atcgccatcc tgatgggaac
    46441 aatcaccgga gtgggcggag ggatggtccg ggacattctg ctggcgcaag tgccgggcgt
    46501 tcttcgcact gaggtgtacg caaccgcagc cttcgcggga tcggcggtcc tgattctgtg
    46561 tcgcaagctg aagctcctgc cgactgtgtc ggcagttgcc ggaggtatag tgtgttttgc
    46621 cctgcgcgtg gtgagcgtgt tgcaacactg gaacctgcca agggtcatgg agccgtaggc
    46681 aatgaccccg aaatggcatg tgtgagacga ttcgatttcc tcgcacaggc tagaacattt
    46741 tgagtatggc tgaactcgga ttggcccagc cgtgcctttt caaaactcat ttgtaagacg
    46801 agctcccggc gagtggaggt gactcgtcat gccatgcgtc acgcgccgcg tccgcccgtc
    46861 tcccgaaaca ggtgcagatt cgaacgcaga atgatgccag ggttcagata gtttgctttg
    46921 ggcaggtccg cgtcatggag taaatgttcg gctaggctgc ggacggcgag ctggccctgg
    46981 cggtagggcc tctgatgcat ggatgcatcg atcgtgccgt caccaaagta gggaaccatc
    47041 tcgcggaata ggtccgttgc aatcagccgc acgttgcccg cgcggttggc ggcagacagc
    47101 gcgcgacaga caggcaggca gttgaccgtg ttgacataaa tgcccgcaag gtcgggaatg
    47161 cgcttcagaa gcttgcgggt tttgttgaaa ctctcttccg ggtcctcgtg ggcctcgatg
    47221 gtcgtcacca ccgaaccgcc ggcgcaatag tcagagaagc tccgagtgaa gccctccgcc
    47281 ttttcccgat gatcgactgt cttcaacatg ccagtcagga tcgccagctt ggaaccgggt
    47341 ggaacgaact tcgacatgag ttcgccggca atcaatccgt tgagccgggg ctcgacgcag
    47401 acgatgcacg atctctgact ttcaggcgca tcggtggata cgcatactac gcgcacacca
    47461 ctttgttccg cggcgtcgat cagcgctgtc atggtctcgg gatttcccgg tgccaccacg
    47521 actccttgca cacctgaatc gacgatcttg cgaaacgccg aacgctcacc tttccccaac
    47581 tcaggaatgg gacggagcac gaactcgatt ccgcgatccg tgtaccggcc cacctcttcg
    47641 tgaatccctt cccagagttc gttgtaaaag tagcggatct cgcggggaac gcagacgccg
    47701 atacgaatgc gccgtcccgt tgaaagtgag cgcgctgcca ggtttggacg gtagccaatt
    47761 ttcttcgcga tatccaacac gcgttgacgc gtctcgtcgt tgatgcccga acgcccgtgc
    47821 agagcgcggt ccaccgtgcc gatggacacg ccggccagct cggcgatttg cggtataccg
    47881 ctgcgttttc ctttcatcgc ggcgagcctc ggactgggat aacttgcata ccgaccgttc
    47941 gctccattta tatcaccgtt aacgtacacg atttagcttt tctttcaagt catttcgcgt
    48001 aataatccgg cggtgggatt cgaagtgtcc catcacgcgg ttgttcggcg gcgtataaat
    48061 catccatatt ctgcaattgg gaaagcattc atgcctgcaa gattgttagg aattgatgta
    48121 ggtacaggtg gcacgcgggc cgtgctggtc gatccacagg gaattgtcgt agcttcgcgc
    48181 acggccgagc acgtagcgtt tgcatcgcca gaaaccggat gggcggagca ggatccccgc
    48241 gactggtgcc gcgcggcctg cgatgcaatc aaacaagtca tggctggttc gggaaccagt
    48301 ccaaacgaga tttctgcagt tggtttttcc ggccaaatgc acggcgccgt gctgctcgat
    48361 gccgcgggcg aagtcttgcg tccagcgttg atctggtgcg atcaacgcac tgccgaggaa
    48421 gcccgcgaat tgactgctca actcggcggg gaccagatca tcgcgtggac gtgtaatgcc
    48481 gcgttgacga atttcacgct tactaagctt ctctgggtgc gaaagcacga gcccaagctc
    48541 ttcaatcgtt ttcgcaccct gctgctcccc aaagactacg ttcgctatcg cctcactggt
    48601 gggtttggaa tggacatggc cgacgcttcg ggaacgttgt tgcttgacgt tgccaatcgg
    48661 cgctggtcaa ccgagatgtg ccgcgctacg gggattgata taaagtgcct tcctcccttg
    48721 ttcgagtcgc cggatgtctg cgcaaaaatc tcggaatccg gcgctgtggc aacaggtctg
    48781 ttgcagggaa ctcctgtagt cgccggcgct ggcgaccaag ccgcgggtgc ggttggactc
    48841 ggcatcgtaa ggcccggcgc agtacatgcc accatcggca cctccggcgt cgtctttgcg
    48901 gcgacggacc ggcctgcact cgatccgaaa ggtcggctgc acaccttctg ccacgccatt
    48961 ccaggccgct ggcacgttat gggcgtgacc caggcggcgg gattctcact gcgttggttc
    49021 cgcgacaatt tcggcgcgct ttctcaagac gcgcgtgatc cctatgagcg gctgtcggag
    49081 gaagcggcgg cggttccacc aggttgcgat ggcgtgttgt gggcgcctta tttgatggga
    49141 gagcgcacac cgcatcttga tccgcaggcg cgcgctgcgc tcgttggact ggcggccaat
    49201 cacggccgcg ggcacattat ccgcgccatt ctggagggcg ttgccttcag cttgcgcgat
    49261 tcgttctcca tcttcgctga gatgaaggtg ccggttacag atatccgact cggaggcggc
    49321 ggagcgcgct ccccattgtg gcgtcagatt caagccgacg tgtatgggca taacgtcgag
    49381 gtggttgagg cggaagaagg cgcagcctac ggagcagcgc tgctggccgg agtcggagct
    49441 ggaacctggt cgagcgtcga tgaagcctgt cagcaatcgg tgcggatcgc gaagcgagtc
    49501 gagcctgacc caggcaccag cgccctcatg aatcaacagt acgaaatgta ccgccgcatt
    49561 tatcccgcgt tgcgggagat ttatcgcaac tcgaattcat cggcggtcac ataagccgcg
    49621 aagaacttga agggctcgct gtcaaacgac aatttcacgc tcccatcgaa cttgcaaagt
    49681 ttgtggcaac tcttacaatg attccggaat ccaattgatc gtccatggcg tcacgcaatc
    49741 gttccatagc agcaaggaga ttcgaaatgc ccgcttcagt tcctgatagc cacaaagacc
    49801 tcttccagaa acgcgcgttc ggcagcttca ccaccctgat gcccgacggc agtccccaga
    49861 cgacgccggt gtgggtggac tttgagaacg gtaaggtgat cgtcaacacg gcgcttggcc
    49921 gtcagaagga caagaatgtg cgccgcgatc cccgggttgc catcaccctg atcgatcctg
    49981 acaacccgta tcgctatctc gaggtgcggg ggaaggtcga cgaaatcacc caagacggcg
    50041 ccgagaaaca tatcgacaag atggcgaaga agtacctcga caaggacaaa tatccgtttg
    50101 cccagccggg cgagcagcgc gtcttgatca aagttacgcc ggacaaattc agcacgatgg
    50161 gatagcttgc agtgtcgttg ccggttaaaa aaacgggcct gtgcccgcct tattttcatg
    50221 ccgatggttt gcgttcgccg ggagtatgga cggagttttc gcgctcgaaa ccgtagggac
    50281 aatggcggca tcccgaggtg cagcaatagc cgcgttcgcg tagatactgg gcagtgaata
    50341 ccacgagccc gttttccgtg tagtagtcaa tgccttctat cggctcgcgc tcgatcattg
    50401 cgccccggcc tcgcgcaaca acgagttgcg ttcctccggg tccactgaaa ccccgtcgcg
    50461 gaacttcaca taccttccag atattgccgg tacccgttcg ccgggaacaa tgaatcccac
    50521 gagattgagc ggatcggctg cggatacgac cagggtctct cccgtcgccg gcaggtttcg
    50581 catggcgcgc agcgattcta ccgcttcggg caaggcgaac tgctcgccca gaaatccgct
    50641 cacaaatcgg ccaccgcgca cctcgccccg gtcctccagg cgacggaagg tgatgagcag
    50701 ctcgcgccac tttggcaggt tcgactcacg cgccaggact tcgcggaaga caactccgta
    50761 gcggcgcagc agcatttggc acatcgcctc gacggccttg ttcctgtccg ctacctcagc
    50821 cgtatagagc agcgaccagc gtccggtgga gtggcgaggc ttggcgattt ttggcgaacc
    50881 atgcgttgca cgatggctgg gggtgatcaa cgatcgcaga ttctcaaagc cgtcggcggt
    50941 caccagtcct gcggcgacga gctcccaaag cgcggtctcg atctcggctt tgagtttgcc
    51001 agtacctcgc acgatgtcgg caaagaacga agcaccgcga cgacggagaa agtccgccac
    51061 ggtcctggca ctctcactga ggacacgggc ctcgttctcg gtgtcgtcgg gaatgcgcgg
    51121 ctgcatccag tcggaatctt cgcgcacgaa gaacgtgatc ggggcgacac tggtgggtac
    51181 gacccgacgt cgtccgttgc cggagtcctc aagtgttgcc gggtgaggcg agacgcgtcc
    51241 ccatcccacc gcgccggtca ggcacagttg gtcgagtacc gccggatcgt aggcgttcat
    51301 gcgcggcttg aggacttgct tctcccacgc attcgcgggg atttcgaagc cctgcaattg
    51361 ccgaatgact tccagcagtc cgcgctcgcc gctggcttgc gcattcggag ccaggtgctg
    51421 ccagcgcaga agccagcgca tgaactgggc ggcgttgacc ggttccactt gtttgcgaag
    51481 cgtggcaacc gtcagatggt gaatgcgcgc cagtagccgt cgatcgcacc attccgccgt
    51541 gtcaggctca gcgcccgcgg cgtggccggt gaagtttccg cgcagcgccg aaccgctcgc
    51601 ctccatacgc agcagggctt gcgccacgtc agccgctgga atgccaagca gaaatccaag
    51661 tgccgctgca ttcgtcggtc cggagtgcat catccaaccc ttgatcatcc ccagcagtgc
    51721 atcgtctcgc gaatctgcgc tcgacggcag ctctggagga gtgacctcga atttcgcatc
    51781 cgggaagatg agtgcaaatt ctttggcgcg ctcggacgcc acgatgtagc gacgattgcc
    51841 ggcaatcgtt cgcaccactc gccagccttc caccaattcc ggcaggaagg gaatccacgt
    51901 cgccaccacc ggctgccatt ccagatcggc aacttcttcg ggcagcgcca ccagcgtctg
    51961 cagggcatcg tgcagttcat ccgcatcgcg cacgtcggga cgcgcttctt cacgtaccag
    52021 ggctatcgct gtttggtcaa gacggccgat ttcgttgagc acagcttcgg gcaacatgcg
    52081 ccgcatctgc accgcgcggg cgcggcgctc ttccagcggc gcgtcgtcga ggaaggcata
    52141 gggattcgcg ttcagtatct cgtgcgaaaa ctgcgaaggc acaggggtat cgaccgcgat
    52201 gcagcggatc gagccgtcac gaatgcccgt cagcacctgg cgcaagccgt cgatatccat
    52261 cgcctcagtg aggacatcct tcatcacctc ttggatgagc gggtggtcgg gaatcttgat
    52321 gtcgccctcg atgttttcct ggcaggccga cacatcggga aaaaccgacg ccagaagatc
    52381 gtcgctgcgg atgcgttgaa tgtatggcgg gaccttcttg ccaccttgaa aacgcaacag
    52441 cgccagggaa cggttggcgt cccagcgcca acgcgtggca aagatcggag acgccagcgc
    52501 ggcctgctca agaatttcct gcaccgtctc cacctgcagg taatggaata cgtcgctgag
    52561 cggaaaacta tgttgctcgg ccagtgcgat gttcaatccg tcgtccgtcg cggccgcttg
    52621 caattcgaag ttgaacgagc ggcagaagcg cttgcgcagc gccagtcccc acgctttgtt
    52681 gaggcgtccg ccaaacggcg catgcagaat gagctgcatt cctccacctt cgtcgaagaa
    52741 gcgctcggcg atgatggtcg tctgcgtggg cactgcgccc agcacggccc gacccgccac
    52801 gatgtattcc actaactgct cggcgcctga atcgtcgagt ccgcactcct ccttcagcca
    52861 agccactgca gcttgcacct caggactgga tgcaaggccg cgaagccgcg tgcgcggttt
    52921 cgcgtccgca gtgccgtcgc gatcagtcgt tggcgccggg agcggcgcgg tgttgggcaa
    52981 catcgcactt acctgttcgc gcagacgggc cacgtattgc gaatgttcag ctgtgcgaga
    53041 aggcgcttct ccccgccaga aaggcacgct gggcggcgcg ccacgagcgt cttccaccag
    53101 catgcggccc gacttcgctt caaccctttt aatgcgccat gaggtatttc ccagcaacat
    53161 gatgtcgccg gcattgcttt cgactgcgaa gtcttcgtcc accgttccta cgacgatttc
    53221 ctcgggttgc accaccaccg tgtacagcga ggtctcggga atcgcgccgc cgctggtaat
    53281 cgccgccagg cgcgctccac gacgcgcccg cagcttgcgg ttgaccaggt cgcgatggag
    53341 ataggcaccg tagcgcccgc gctgggaggc gattccttgc gacagcattt ccaggatgcg
    53401 ctcgaattca tcctcagtca gcgcacggta gggataggcg cgtcgcacgc aatcgaacag
    53461 cgctgcctcg tcccagtctt cgcaactgca catggccacg atctgctgcg ccagaatgtc
    53521 gagtggcttg tcgggtatct ggatgcggtc gaggtcgcca cttcgaatcg cgaggacagc
    53581 ggccgcgcac tcgaccatgt cgtcgcgcgt ggtggcgaac aggcgacctt tgggaatcgc
    53641 tccgcgccaa tggccggcgc gtccgatgcg ctgcaatgcc acagaaattg cgcggggcga
    53701 actaatctga cataccagat cgatatttcc gatatcgatg cccagttcca gcgatgcggt
    53761 cgccaccagc acgcggattt cgccattctt gagtcttcgt tccgccgcta gccggagctt
    53821 tcgcgccaag ctgccatggt gtgtggccac caggtcttcg ccaacgcgct cgctcaagtg
    53881 gagagccacg cgttcagcaa ggcggcgagt gtttacgaag accagggttg agcggtgctg
    53941 ttgcaccaac tccgcaatgc ggtcgtaaat ctctccccac atctcattgc tggcgacggg
    54001 cccaagttcg gaagccggaa cttcgacggc gaggtcgagt ttgcgccgat gtccaatgtt
    54061 caccacggcg ggcgccggac ggcccgcgcc ggtgagaaaa tgcgccacca actcaatcgg
    54121 cttctgagtc gctgacagac ctatgcgcgt tggctcacct gcgagcaggt cgaggcgctc
    54181 cagcgacaaa gcaagatgtg cgccgcgctt gtcgtcggca acggcgtgaa tttcgtcaac
    54241 gatgacggtc cgcacatcgc ggaggatggc gcggctcttg tctgcggtga gcaaaatgta
    54301 gagggattcc ggcgtggtga cgagaatatg cggcggacgc tttagcatgt cgcgccgctc
    54361 cttcgccagg gtgtcgcccg ttcgcacggc ggtgcggatc tcgggcatca acatcccgcg
    54421 ctcggccgcc aacgcgagga tttcgctgag cgggccttcg agatttttct ggatgtcgtt
    54481 gcccagggcc ttcaacgggg agacgtacag gacctcggtc cgattcacca gcgtgccgtc
    54541 gagcgcttta cgaaccaggc gatcgatgca gatcaaaaat gcggcgagcg ttttgcccga
    54601 accagtgggc gccgaaatca gtgtcgtgcg tcccgccagg atttccggcc agccctgctc
    54661 ttgcggttcg gtgggcgagc caaatttcgc cacgaaccac tccgccacca ggggatgcgc
    54721 ccaggcaagc gaaaccggaa tagcactcaa aggcatgctc gacattctag ccgcttttga
    54781 aactttcgcc aattgttcgc caccaggcag aagaattttg acgttataac ttaagctata
    54841 acgtcgggcg caaaccgcga aggagcctga gaccgaatta gcgagtcaaa tcggctgtta
    54901 agatgacgcc atgtccgaag aacgcgccaa aaagatcaag ctgatgattt ttgatgtcga
    54961 cggagtgatg accgacggaa cgatttttct gtttcccgcc ccagcgggaa catcgggttc
    55021 cggaacccag gaacaccgcg acagaatggc cgacaaagtg ggctttggca tttccagttc
    55081 aagcgtggtc gaagccaagg gattcaatgc ccatgacggc accggatttt cgctggcgcg
    55141 cctgggcggg ctcaaggccg ccattatcac caagcggatt tctgagacgg tcgctctgcg
    55201 cgcccgcgac ctgaaaatcg agcatgtgta ccagggacag caggacaaag cggccgcatt
    55261 ctatgaaatc ctcagcaaag aaggccttca ggctgacgaa gtctgctacg taggagacga
    55321 catcatcgac cttccggtga tgcggctgtg cgggttggcg attgcggtaa agaactcgcg
    55381 cgaggaagtg ctgcgcgagt cgcactacat cacgccgcac gaaggcggca aaggcgcggt
    55441 gcgggacgcg attgaataca ttttgcgagc gcaaggatcg ttgcagcgga cgattgacga
    55501 ttacgtttac tcgcgcagcc aggacccggc cgccaaagtg cagtaatcgt gcagtgaaaa
    55561 aacagagccc ccgccccccg atgcacaggg gcgagttgca catcggaggg tagccgcctc
    55621 gttcgcgacc gttctcaggg ttgatactgc tgagaaacac gcacgatttc ccgaagtttc
    55681 gcgattcacg gaataacttt tcccaatcgc tgtcgctgca ccaaccgttg tccgaattgg
    55741 tatcgtgagt tcaacggttg ccaaacggta tcgttcacgg cggggaacca taagtggtac
    55801 ggcgccccag ccgttttgta tggtaagtaa taaagggtat acaagcttgt gccgttagtt
    55861 tatctttcgg tactcgctgc gggatatcgg gacgcacttt tccgcggttg aaccgttgct
    55921 ggcatggcct gaatcaggca gccagcattc acgcgctaca tgcttgtgaa actggggttg
    55981 ttatcaggat cggaatgaag aatagcgtgg ccatttacag tttgctgctc cttacagtcc
    56041 ttgtgctgcc tggcacttta gcgtcggcag ccaccattgc tgcgcccaag aagccccacg
    56101 ctaaatcgcg gcatgtgacc gaaagccccg cggcccagaa gaaaatggcg cgcatgaaac
    56161 acagcagtcg tacgactgcc agcgtcaatc gatcgagcca cgtggtggtc gccaagagcg
    56221 ggaaaaaggg caagaaggcc acgtattacg agcggttcca tgccagctct tttgccgacg
    56281 accaggttga tggtgacatc gccaccggcg aggacccgat tgtccgagcg gcggccatcg
    56341 acgcactcgg caacatgaat ggaacggtcg tctcgatcga tcccgaaagt ggacgcatcc
    56401 tggcgatggt gaatcagaag ctggcgctgt cggctggcgc ccagccgtgc tccaccatca
    56461 aagttgcggt tgcgctggcg gccctcagtg aaaacgtgat caccaaggac actgagattc
    56521 cgctcggcaa gtattaccgg atggacctca ccaccgcgct ggcacattcg aacaacgcgt
    56581 attttgctgc cgtaggacag aagctgggtt tcgagaaagt gagctactac gcgcaacagt
    56641 tcggactagg cgaactcgcg ggatataaca ttccaggcga acaccggggc acgtttcctt
    56701 ctaccgaact ggatgccaaa ctcggcgggg tagggaagat gtgttccttc ggcgagagca
    56761 tctcgctcac gccgttgcaa ctgggcgcgc tcatggctgc catcgccaac ggcgggtctt
    56821 tgtactacct gcaacacccc acgacggcgg aggaagtcgc gaatttcacg cccaagatga
    56881 agcgcaagct ggatattgga ccgattattc ctgagctttc agacggcatg tcaggcgccg
    56941 tcgaatatgg cacggcccgg atgcttcgca gcagtttcgt ggaagaaccg gtgctgggta
    57001 agacaggcac ctgctcgaaa gacggcacgc gctttggctg gttcgcctcc tatgccaata
    57061 ctgactacgg acgcatcgtc accgtgatct tcctccaggg tggccgtccg accttcggtc
    57121 cgaaagcagc cgagctcgca ggacacttct accgcaatct ctacgatcac gactatttcg
    57181 ctgccaaagc ttccgcgata cccgttcgct cggcgggtgc gacggcgatt ggagtggtcc
    57241 actagtcgat tgaacaagtg ttagtgagcc tcccgcgtgg gaggcttttt tgttggtccg
    57301 gtgatgcccg atgtcacttg gctaaaggcg atggtttttg cggctgcaaa tcctgcacag
    57361 cgtgctggta agtcaacttg gcggcagggt cctgggcgtc tgcgagtttc ttgttcagcc
    57421 attccagaac ggccgcgcgc gagggatggg gatagttcgg gtcggcgtcg gtaatgcgcc
    57481 aatcgccgtt cacttccgaa accacaaact ggacatcctg gaccgtcttt gcggactcgt
    57541 ttgaatagcc ctcggccatg tcgtacctgc caagcaaacg atagtgaacc gtgaccatgt
    57601 acttgccgtg tcccggcgtc ggctcgggat caacgtcgaa ccgcaccgtc accatcatca
    57661 atggaaattc agggtttgcc cgccaggaaa ccaggggttg cactttcggc cactgcgacg
    57721 gatcaagccg tgcgccagcg taatccattc tgcaaaagcg ctcaaccagg tcccgcgcat
    57781 tttcagaatc ctgcttcggg tcagagcggt tcagagggat tccgaactgc gcctgggcgc
    57841 caattgcagc tgccagcagc aatacgaatg caacgcgttt gaagagaaac aacactctac
    57901 cccgagtctg cagtatacga ctcgagtagc ataccgcgtt ttgggccgga agaacgaaac
    57961 gcccagcgtt accgccgggc gtctcgcgtc aaaaggaaac tgattcctac gctcgtgctg
    58021 ttttctggtc gctgaagacc cgcccgtcct tgatgtgaat gatgcgatgg gcatactcgg
    58081 cgatatcgtg ctcatgggtt accagcacaa tattgttgcc ttgctgatgc aggcggtcaa
    58141 acaacgccat gatctccacg ccggtttgcg agtccaggtt tccggtgggc tcgtcggcca
    58201 gaatgatgga cggcgtgttg accagcgcgc gtgcaatcgc gaccctttgg cgctggccgc
    58261 ccgacaattc gttgggcttg tgattcatgc gcgattccat gccaaccgac tgcagtgcct
    58321 gtttggagcg ctcgatgcgc tcctgcgccg gcacacccgc atagatcagc ggcagctcca
    58381 cgttatggag cgacgtggct cgcgccagca ggttaaatgt ctggaatacg aagccgatct
    58441 ccttgttgcg gatgcgggcc agttcgtcgt cgtccaattc actgaccaac tggccattca
    58501 gccagtactg gcccttgctg ggcgtatcga ggcagccgat caggttcatc agcgtcgatt
    58561 ttccggagcc cgacggaccc atgatggcga catactcgcc ccgctgaatg cgtacgttca
    58621 cgccacgcag cgcgtgcact tgttgctccg agcccatgtc gtaggtcttc cacaggtctt
    58681 cggtgcagat gatgcaccct tccggcggtg cgccggtctg tggttccgta agactcacca
    58741 gttcggttgc catcctgtgc ttctcctcga ttggtagctc gtcagggttg ctcattgccg
    58801 ccgattcccg tgccttcctg ccggcttggc cggtcgcatg aggtctagga ttcttctttt
    58861 ttggaccctg tcgtattgtc gactttcacg ctggctccgt tgcgcaacgt gcgaagaact
    58921 ttgtagcttc cggtgacgat ctcgtcacct tctttcaggc cgttggtaac ctcgatatcc
    58981 gtggtcccac tgatgcccgt tgtaacaggt acgaaaacgg cttccttttt gttcttgaga
    59041 acaaaaacgc cctgcagctc ttctttcggg ttgcccttgg taagcgaggc cgcttgtacc
    59101 gccgcttcct ttgacttctt ctccggctcc agttcgctgc gctgccggat ggtcaacgcc
    59161 tggatcggaa tcgccaggga gctgttacgg gaagccgtgg tgattcgggc tgtggttgaa
    59221 agtcccggca acaagtttga cggcgggtct tgaattgtaa ccacgacctt aaaatccttg
    59281 gcctcctggc tactcgatgt cgattgtgcc gtggaaacgc cggttgacct gaggattgcg
    59341 ttattgccaa tctcggtgac gatgcccttg aaccgctgtt tcgggatggc atcaatcgaa
    59401 acctcagccg tctcgccaag ccttacattc acgatgtcgg tttcgtcgac cttcacttcc
    59461 gccgtaataa cggagacatc ggcgatcgtc atcaaggtgc ttcccggcgc gttctggatg
    59521 ccgataacca cagtctcccc ctcgcggacc ggaaggttcg taataactcc gtcgaagggc
    59581 gccgtgtact cggtcttgct cagcacgtcg gtcacgcgtc gaagattcgc ggcgttctgc
    59641 ggaatcctgc gctgcgccga atccatctga gccttggctt gcgctaattt ggcttttgcc
    59701 tgtcccagtt gagctgttgc cgttccatag gccgctttct tggtgtcgta atcggccttg
    59761 gcgatcaatt gcgccttata caatgcctcg ctgcggtcgt aatccaattt ggcccgctca
    59821 aactccgact cggcctgatt ctggtccatg gtggccgtat tcacagcggc tttggcggcg
    59881 tcagcatcgg tcaccgcagc gctgagtgcg gcctgattgg cggacacatc cgccgacgac
    59941 tgcacgtttt ccagggttgc cagcatctgg ccctgtttca cgcggtcacc ctctttgacg
    60001 tagaggtgag ttattttgcc gaaagcgttg gctccaatat tgacgtaaac cttaggcttg
    60061 atttcccccg atgcggtcac taccgacgtc aagtcctcgc ggaccacttt gccggtctgc
    60121 accgtcacca ctcccttacg gctttgctgg atcgtaaacc caacaatagc cagcagtatg
    60181 acaactgctc cagcaatgag tgcgactttc ttccagttct tcattcatcc tcggtcagac
    60241 atacctgcct cgcatccgta cttgaatgtg caacttagta cgaactaaga cggtggaaag
    60301 ttccttagga aagcagagtt tcgagaccgg attcgtcagt tacgacgact cggcggcggc
    60361 ttggtcgtag cttcgctcac ggacaggatc cgggactctc acgaatacca cgattatagc
    60421 cgctccgttc gcaggaatcg atattgttca cggaatatgc atacgctgaa tctgaaccgg
    60481 ttcgaggggc gctttcgtcg gaagaagcga gcgactttct gcagcccaac gggttaagaa
    60541 acgttagggt tcgggggttt cgtggcaacc ggcgggggat gtccgcgatt ccttgctttg
    60601 cccgttgcta gatcatacaa tttaagggct cgaagtgttt cctcaggagt ccgttggatt
    60661 acatgaaggt ccgcattttt cgtccacttt tttcgttgct tgtgatcacc gcattcgctg
    60721 gatccgtggc cgtcgctcag agttcggctc ccgcggcagc cgacaagtcg gccagtccgg
    60781 ctgcctccga tacgaaggcc ggcactgccg ttgcccctgc aaccacagcg cccgcggacc
    60841 ctcaggcaga ccccctgaag cgccccgtca atgaaaagca gaaaaaggcc aacgccaagg
    60901 ctctgaagca ggaattgggc tcgacttaca aaaagtggct caacgaagac gtccgctgga
    60961 tcattactcc ggaagagatg tcggccttca agcagctctc aaacgacgag gagcgcgacc
    61021 agttcattga gcagttctgg ctgcgccgcg atccaacccc tgacaccgaa gagaacgaat
    61081 tcaaggaaga acattaccgc cgcatcgctt acgccaacga gcatttcgcc gcaggcaagg
    61141 ccggctggag gaccgatcgc ggacgcatct acatcgtctt cggtccgccc gacgagattg
    61201 acgctcatcc ctccggcggg cagtacgacc gtcccatgga agaaggcggt ggcgagacct
    61261 cgacttatcc cttcgaaacc tggcgttacc gctacatcga gggcttgggc gacaagggcc
    61321 agcaagtcat gatcgagttt gtggacacct gtatgtgtgg tgagtaccac atgaccatcg
    61381 atcccaacga gaaggacgct ctgcttcata ccccgggagc aggtctgaca acgtacgagg
    61441 agatgggcat caccaacaaa gccgaccgca ttgcgggcat tggtagcagc actctcgggc
    61501 caatgtctgg tttggacaat accaaacagt tcgacaagct ggatctctat gccaagctca
    61561 ataccgctcc caaggtgaaa ttcaaagatc tggaagaagt tgtcagccac aaaatcaatg
    61621 tcaacctgat gcctttcgac atcatgacgg actttgtgaa aatcactggc gacacggtgc
    61681 ttgtcccggt gacgatacag gtcaagaaca aggacatcac ctttgtgaac aaggacggca
    61741 tccagcgcgg caccgtcaac attttcggtc gcgttaccgg ccttaccggg aaaattgcgc
    61801 agacgttcga agacacggtg caggtggatg ttcccaccga attgctggaa aagacccagg
    61861 aacaccagtc gctctactgg aaggccctgc cgttgcgccc cgggcgctac cgctttgaca
    61921 tcgtggtaaa ggatgtcaac ggcgatcgcg tcgggacctg gagccacggc gttgcggttc
    61981 ccgagtacaa cgaagacaag ttggcttcat cgtcgttgat actcgcggac cacatggaaa
    62041 aagtcccggc caagagcatt ggtgcaggaa atttcgttat cggccagact aagttcgcct
    62101 atcctcacct ggagccggcg aacggctcac cggccacctt caaacgcgac cagcgcctga
    62161 atctgtggtt gcagatatac aatctgcaag ccgacgcgaa gaccaacaag ccatccgcca
    62221 aggtcgaata tgaaatcgta aacatggcca ataaccaggc cgttgtccat tcttcggaaa
    62281 ccaccgatac aatgggtaac ttgggtgacc agatgacgct ggagaagagc ttggggttga
    62341 gtggctttca gcctgggatc tatcggttga cggtgaaagt ggacgataat atttcgaaac
    62401 agcaaattgc gccttcggtg cgctttgctg ttgaataacc ttgctgtagt accggccggc
    62461 ggtcgctgct ttcggcccgc tgcatttttg aacccgggaa tccattgttt gggggtgtgc
    62521 tgatgacccg aatgttggga tggcttgcgg ttgcggtctc gctctgcatc agtgcagggg
    62581 ccgggacagc tccgggctcg atctcgggat ctgtgaaaga ttcctcaggc gtagcccaga
    62641 tgggcgcggt tgtggagatg ctcgctgttg gcacgggtca gcacgtgctt gccttttccg
    62701 acgccgacgg ccactttaac cttgccggtt tggccccggg caactacgat ctgcgggtta
    62761 ccgctccttc gtttctgccc accgtgcgcg aagacatcgc cctcgccgca ggtgccacca
    62821 agatcgttaa catcacgctg aataccctgt ttgaggcggc tcgtatggtg ccgtctcgca
    62881 agcgcgacaa cgaggacgac gacagttgga agtggacgct tcgttctacc tccaatcgcc
    62941 ccatccttcg cttcgacgac ggtatcccgg tggtcgtgga ggctgggcag tcggaccacg
    63001 ggctgaaggg ctcgctagcg ttcatggccg gtggctctaa cgagggttac ggaagcgctt
    63061 ccgatcttgg cacgtcgttc acccttgaac agtcgatctt cagcactggc attttggcgt
    63121 ttgatggcaa cgtgggatac ggtgccggca cgggtactcc cgacggcatt ttgcgcgctt
    63181 cttacagtac tagcggtggc gagaacggct cgccccacca gctcgcgttc accatgcggc
    63241 gcttcaccgg cccggatctg ctggtacatc gcgggtctct gcaggcgctg gcgttgtcgt
    63301 cgtacaacac gttctcgctt ggcgattcgc tcgacctgca atatggcggg gaactgcaga
    63361 ctatccagtt catggggcgc gctaatgcgt tccggcccta cggctcgatc gactggcatg
    63421 tgggcgacaa cacggtcatt gaatatcgct actccaccag cgaacccacc acgcgtgcca
    63481 gcaagggatt cgattctgct ccggcggact ttagcgagag cggccctcgt atgacgttgt
    63541 tgaatggcgc ccctctgttg gaaaacgcgc atcaccatga ggtttcgatc tcgcagcggt
    63601 tgggcgatac gcgcctgcaa gtggcctatt tccgcgaccg aatcaaggac ccggaattgc
    63661 ttggtgtcgg cgatatggtg accgacactg gtgatgtgct tcccgacgtg tactccggca
    63721 cgttcagcta caacggcggc gcgctaaacg cacagggcgc ccgcctcgtg gtgcagcgaa
    63781 agctgacgcc cgatatgacg atgacgctgg actatgccta cggcggcgta ctcaacctgg
    63841 agcagcccgg cgtcgactgg gacgccattc atgccgattt gcggcaagtc tggcgccact
    63901 cggcagccat caagctgaac ggcaccgttc ctcgctggaa taccgagtgg attgtttcct
    63961 accgttggac cagcggcggc gcgcttaccc cggtcgatct attcaacgcg tcgacgggcc
    64021 agacggacgg attcttcaac ctcttcgtcc gtcaacccat gcctcgcgtg catttcatgc
    64081 cgggtcgcat ggaagcctta attgacctgc gtaatctgct ggcgcaaggt tacgtgccag
    64141 tgctcggacc cgatggcagt accgtgtacc tggtgcagtc ggcacgcagc gttcgcggcg
    64201 gcgtggcctt caccttctga ttttctcgct tacacgacaa agcccgctcg ttaagctggt
    64261 cgtcttgatt ctgctactcg gcgtcacaac gcttctaccg catcgcatct tcgacttcgc
    64321 tcagccagtc aggacgcttc agcacttccc gtggcttcgg cccgtcagct gcgcccacga
    64381 ttccatcacg ctccatgagg tcgatcaggt gggccgcgcg tccgtagccg atccgcaacc
    64441 ggcgctgcag cagggaagtg gaggccttcc cgtattccag cacgatgcgt acggcgtcct
    64501 ggaacaactc gtcctggtcg ccgtcaccat cgccatctgc gcctgaaccg ccgccgtcac
    64561 tatcttcccg cggag
//
LOCUS       DASRKK010000578         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_344202, whole genome shotgun sequence.
ACCESSION   DASRKK010000578 DASRKK010000000
VERSION     DASRKK010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344202"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFC15_13660"
     CDS             complement(<1..724)
                     /locus_tag="VFC15_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11247.1"
                     /translation="MTHLRKIMLEELHRRNYAQTTIDCYVRTVEHFSRYFQRSPEQLG
                     PQHIREYQAALFKKWKLAPNTVNQRLAALRFFYIQTLKRAWSVAETPYPKKVLKLPII
                     LSQEEVARLINSALTPFHRIVLMTLYATGVRRAELARLKISDIDSQRMVIHIQGGKGR
                     QDRDVMLSPKLLDALREYWRGLKRKPSRWLFPGGLSHTADRPITPKTVYHACRQAARR
                     AGLQEKNIHPHTLRHCFATHLLE"
     gene            1055..>2007
                     /locus_tag="VFC15_13665"
     CDS             1055..>2007
                     /locus_tag="VFC15_13665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM11248.1"
                     /translation="MIETTISHYRILEKLGGGGMGVVYKAEDTRLHRFVALKFLPDGV
                     AQDPQALARFAREAQAASALNHPNICTIYDIGEEDAHAFIAMEFLEGQTLKHRIARRP
                     LELEQLLPIAIDIADALGAAHAKGIVHRDIKPANIFVTERGHGKILDFGLAKVTFKAG
                     EGSAESETVISDVDAEHLTSPGAMLGTVAYMSPEQVRAKELDARTDLFSFGSVLYEMA
                     TGKLPFDGSTSGEICGAILHKEPPPPSQVNPQVSAGLEAVIGKALEKDRNLRYQHAAD
                     LRADLQRLKRDTDSSQHSTAVATEYEADAAGPGATGRNSSS"
BASE COUNT          425 a          566 c          607 g          409 t
ORIGIN      
        1 cttctaacag gtgggtggcg aaacaatgcc gcaaggtgtg gggatggatg tttttctcct
       61 gcaggccagc gcgtcgagcg gcctggcggc aggcgtgata aacggtcttg ggggtgatcg
      121 gacggtctgc tgtgtggctg agtccccccg ggaacaacca ccgggagggc ttgcgcttta
      181 agccacgcca gtactcgcgt agtgcatcca gcagctttgg gctgagcatg acgtcacgat
      241 cctggcgtcc cttgccccct tggatgtgga tgaccatccg ttggctatca atatcgctga
      301 tcttcaggcg tgctagttca gcacgccgca cgccggtggc atagagcgtc atcagtacga
      361 tgcgatgaaa aggagtcagt gccgagttga tcagacgagc cacctcctcc tgactgagaa
      421 tgatgggcag cttcagcact ttcttgggat agggagtttc ggccacgctc caggcccgct
      481 tgagggtctg gatgtagaag aaacgcagag ccgccaaccg ttggttcacc gtgttcggtg
      541 ccaacttcca cttcttaaac aatgcggctt ggtactcgcg gatgtgttgc gggcccaact
      601 gttcgggcga acgctggaag taccgggaga agtgttcgac ggtgcgaacg taacaatcga
      661 tggtggtctg cgcgtagttc cgacggtgga gttcttcgag catgatcttg cgtagatggg
      721 tcacagagat gcctccttct ctgtgacaca acttagatca actcacctgt gctgtcgcat
      781 ggaccacgca cgcgcaagcg tccgccgtac tacggcttcg ttcaagtcgt cttctggtcc
      841 atcactctat tcgggaggtc ctgctgtagt gcatgtccgt aacgccacga ttcgggagac
      901 gagggcttta tcggatcagt ctggaggcat tacatcgaga tcttggggct tgcttgccga
      961 taaaagatgg tgggggcggc cgcgccaggc agtgaattga ctcattggga gttcgctcat
     1021 actatcatca tgccacttgc atcgtacggg caggatgatc gaaacaacca tctcgcacta
     1081 ccgaattctc gaaaagctgg gtggtggcgg aatgggcgtg gtgtacaagg ccgaagacac
     1141 gcggctccac cggtttgtcg cgctcaagtt cctgcccgac ggcgtcgccc aggatcccca
     1201 agccctcgcc cgttttgcgc gggaggcaca agccgcgtca gcactgaatc atcccaacat
     1261 ctgcacgatt tacgacatcg gtgaagagga tgcacatgcc ttcatcgcca tggagtttct
     1321 ggagggacag accttaaagc accgtatcgc gaggcggcca ctcgaattgg aacaattgct
     1381 tcccatcgct atcgacatcg ccgatgcgct tggcgcggcg cacgccaaag gtatcgtcca
     1441 ccgcgatatc aagccagcca acatcttcgt aacagaacgc ggccatggca agattctcga
     1501 ctttggactg gccaaagtga cattcaaagc cggggaaggt agcgccgaat ccgaaaccgt
     1561 catctcggac gtggatgccg aacatctcac gagtccggga gcgatgctgg gtacggtggc
     1621 ttacatgtcg cccgagcagg tgcgcgcgaa ggaactggat gcgcgtactg acctgttctc
     1681 attcgggtcg gtgctctacg agatggcaac gggcaaactg cctttcgacg gatcgacctc
     1741 gggagagatc tgcggtgcaa tcctgcacaa ggagccaccg ccgccatcac aggtcaaccc
     1801 gcaggtttcc gcggggctgg aagccgtgat cggcaaggcg ctggagaagg accgcaacct
     1861 gcgttaccaa cacgccgcag accttcgcgc cgacctgcag cgcctgaaac gcgacactga
     1921 ctcatcgcaa cattcgactg ctgttgccac ggagtacgaa gcagacgcgg ccggaccggg
     1981 ggcaacaggc aggaactcca gttcgaa
//
LOCUS       DASRKK010000579         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_832753, whole genome shotgun sequence.
ACCESSION   DASRKK010000579 DASRKK010000000
VERSION     DASRKK010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832753"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VFC15_13670"
     CDS             complement(<1..1543)
                     /locus_tag="VFC15_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZM11249.1"
                     /translation="MPSTRKTAKSATVSSAAKPATAAKATVAVPAEPTGSKGRIYLID
                     TMSFIFRAYHAMARQRPMSTKTGIPTSATFVFVNMLRKLRADFEPQYLAAVFDVAAPT
                     FRDEQATAMPSLRKFDSKTQTYVDVAYDGYKANRAEMPADLAQQIPYIRRALEAYRIP
                     MIEASGFEADDVIGTLARQAADAGYSVFVVSSDKDMMQLVSERTCVLNPPKDNLICDA
                     AKVEEILGVPPDKVVDVMALRGDAIDNIPGAPGIGDKGSVELIQRFGSVENALEHAEE
                     VERKAYRESLQNNRDMILLSKQLATIKTDVAVGFEPEAMRAQEADAAAARALFTELEF
                     TTLVQDFLHESVELGETDYRDAQSAAEVEAVVALASKPGAVLAIALESAVQPTDVAEE
                     ESEEEQGNEQLSLSATPQIAPVPATLRVAISAEPGKALTLALDDSEVAQPLKRALADE
                     TIPKTVHDYKSAMHVFGEHGLTLAGVQHDPLLYSYLLNPTYPNAASYGLSQTAQRHFS
                     LILGPS"
     gene            complement(1690..2883)
                     /locus_tag="VFC15_13675"
     CDS             complement(1690..2883)
                     /locus_tag="VFC15_13675"
                     /inference="COORDINATES: protein motif:HMM:NF027613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM11250.1"
                     /translation="MRIILDSNVYVADFRMEGIAFSNLFDFIRKTRSTLVLPRIVREE
                     VVHRYIERFTAQVRETGKSWKLFRYLMLDHDPAEFQKPDLKYQVRELRKRLRTPVKGV
                     AIQYCPDVSGVDINEVAIRGIRRIPPASLAGEELRDVIIWYQVLSVSKGAGDMTAFVT
                     SDCGFWDGESLKAQIQQDIIGSKAKIEVYKGIDSFIKANSPRHTELTTESATQLVPAS
                     LFSELVAPVFATSLKRAARSSLMYEIFGCYGGNVGVLELKPMASRFLDGTIYEIDPTV
                     SFVTAIYEYKSNAVLKLGPDPAWNGPTGTSLMGISSLSANPLWLSTQEKIASMKDSER
                     VKEVTAVGRASVSARMVSGLVTEKAMENFALTKVEIEDKEVEISEVIGPRGPWLRATT
                     KETSR"
     gene            complement(3017..>4319)
                     /gene="queA"
                     /locus_tag="VFC15_13680"
     CDS             complement(3017..>4319)
                     /gene="queA"
                     /locus_tag="VFC15_13680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZM11251.1"
                     /translation="RPKIVYHGGHGVFGFFRIFSVISVPLWLIFIPIGGPQETLRHNR
                     FVLVSDFDYRLPEELIAQEPLSDRAAARLLRLSRSDDRLDDLVFRDFPQLLRPGDLLV
                     FNNTKVLPARLFGHRSGSRAQRLSTQNPATKDFLRGRVEVLLTKQLSAHPPEWEALVH
                     PGRKLGVGERIYFGGEADESSHELAAEIVGRGTFGERHLRFDPVDDFFGVLNRIGHIP
                     LPPYIDRPDTPSDRECYQTIYAKPLGSVAAPTAGLHFTPEILERIRERGIEMAEITLH
                     VGLGTFQPIRVEVVEEHKLHREWYEISADAAQRIQSAREEGRRIVAVGTTTVRTLEFA
                     ARTGPPKQNQLGACPELAEGWGTPDGRIAAQSGEADIFIYPGFEFRVVGAMLTNFHLP
                     KSTLLMLVAAFAGRERILRAYEHAVRQQYRFYSYGDCVFVE"
BASE COUNT          908 a         1236 c         1239 g          936 t
ORIGIN      
        1 gagaaggacc caatatcaag ctgaagtgcc gctgggcggt ctgcgacaac ccgtagctcg
       61 cagcgttcgg gtatgtcgga ttgagcaagt agctatacag cagggggtcg tgctgcacgc
      121 ccgcgagcgt caggccgtgc tcgccgaaga catgcattgc cgacttatag tcgtgtacgg
      181 tctttggtat cgtctcatcg gcaagcgccc gcttcagggg ctgggcaact tccgagtcat
      241 ccagggctag cgtcagagcc tttcccggct cggcagaaat cgccacgcgc agagttgccg
      301 gtacgggcgc gatctgcggc gtcgctgaca gggagagctg ttcgttgccc tgctcctctt
      361 cactttcttc ttcggcaaca tcggttggct gcaccgccga ttctagcgcg atggcgagca
      421 ccgcccctgg cttgcttgcg agcgccacaa cagcctcaac ctcggctgcg gattgagcat
      481 cgcgatagtc ggtctcgccg agttcaacgc tttcatgcag aaagtcctgg accagcgtcg
      541 taaattccag ttcggtaaac aaagcgcggg cggcggccgc atcggcttcc tgcgcgcgca
      601 tggcttcagg ctcgaacccg acggcaacat cggtcttgat cgtcgccaac tgcttgctca
      661 gcaggatcat gtcgcgattg ttctgcagcg actcgcggta ggctttgcgc tcgacttcct
      721 ctgcgtgctc cagcgcattc tccacgctgc caaaacgctg gatcagttcc accgaacctt
      781 tgtcgccgat gccgggtgcg cccgggatgt tgtcaatggc atcgccgcgc agcgccatta
      841 cgtccaccac tttgtccggc ggcacgccca ggatctcttc cactttggcg gcgtcgcaga
      901 tgagattgtc tttgggcggg ttgagcacac aggtgcgctc gctgaccagt tgcatcatgt
      961 ccttgtcgct ggagacgaca aacaccgagt agcccgcgtc cgctgcttgt cgcgccaggg
     1021 tgccaatcac gtcgtcggcc tcgaagcccg aagcctcgat catggggatt cgataagcct
     1081 caagcgcgcg ccggatgtag ggaatttgct gcgcaagatc tgctggcatc tcggcgcgat
     1141 tggccttgta gccgtcatat gccacgtcaa cataggtctg cgtcttgctg tcgaacttgc
     1201 gcaggctggg catggcggtg gcctgctcat cgcggaaagt gggcgcggcc acgtcgaaaa
     1261 cggcggccag gtattgcggc tcgaagtccg ctcgcagctt gcgcagcatg ttgacgaaga
     1321 cgaaggttgc gctggtgggg atgccggtct tggtggacat gggccgctgc cgcgccatgg
     1381 catggtaggc gcggaagatg aacgacatcg tgtcgatcag gtagatgcgg cctttcgacc
     1441 ccgtcggctc agcgggaacg gcaacggtgg ccttggctgc ggttgccggt ttcgcggcgc
     1501 tggagacggt tgcggatttt gcggttttgc gagtggaggg caagagaaaa ttctaacaag
     1561 tcgcggcgaa ccctgtcaca gaaaacgctc caagtatgat gaacgccgct gaaggcatct
     1621 ttgatgcaac aatggtgacc aagcgcttga cactggacgg tgacgcctaa tcggccttgg
     1681 cgtttcgtgc tagcgtgacg tttctttggt cgtagctcgc agccaaggtc ccctgggacc
     1741 aatcacctca gaaatctcaa cttctttgtc ctcaatctca accttggtga gagcaaagtt
     1801 ttccatggct ttttctgtaa ccaggccgct aaccatacga gcggagactg aagcacgccc
     1861 gacggcggtc acttctttga ctcgctctga atccttcatc gaggctatct tttcctgagt
     1921 tgataaccac agcggattgg cggacaggga tgagattccc atcaaagaag tgccggtcgg
     1981 cccgttccaa gcgggatcag ggccaagctt tagcacagcg ttgcttttgt attcatatat
     2041 cgcggtgaca aagctcacag tggggtcaat ttcatatatc gtgccatcca gaaaccttga
     2101 tgccattggc ttcaattcaa ggacaccgac atttccgcca tagcaaccaa aaatttcgta
     2161 catcagggat gaacgggcgg cccgtttgag gctcgttgcg aaaaccgggg caacaagttc
     2221 cgaaaacaat gaagcaggaa cgagttgagt tgctgactca gtcgttagct cagtgtgccg
     2281 aggtgaattg gctttgataa agctgtcaat gcccttgtac acctcgattt tggctttgga
     2341 gccgatgata tcttgctgta tttgagcctt taacgactct ccgtcccaga atccgcaatc
     2401 gctagtgaca aaggccgtca tgtcacctgc acctttggat acagaaagaa cttgatacca
     2461 aatgatcaca tcgcgcagct cttcgccagc tagactcgcc ggaggaatgc gcctaatgcc
     2521 tcttatcgca acctcattga tatccacccc agaaacgtcc gggcagtatt gaatagcaac
     2581 tcctttcact ggagtcctga gtctctttct caattcgcgc acttgatact tcaggtcggg
     2641 cttctgaaat tcagcgggat cgtggtccag catgaggtat cgaaataact tccaagattt
     2701 cccggtttca cgtacctggg ccgtaaaccg ctctatgtac cgatgcacaa cttcttcccg
     2761 cactattcgc ggaagaacga gtgttgatcg tgtctttcgg atgaaatcga acaggttaga
     2821 aaaagcgatt ccctccatgc ggaagtctgc cacatagaca ttagagtcaa gaatgattcg
     2881 catgtttttt gccgtttctt cccatacaga gtgtatagcg tcgagaatac ttcatcagcg
     2941 gcctcgtcat tactctcgat aacagctcat tgcaccacca attcctggcc atcagcgcgg
     3001 attcggaagg cgcgccctac tccacaaaca cgcaatcccc atacgaatag aaccgatatt
     3061 gctggcgaac ggcgtgttcg taagcccgca agattcgctc gcgcccggcg aaagctgcga
     3121 ccagcattag tagcgtcgat ttcggcaagt ggaagttagt cagcatggcg ccgacgacgc
     3181 ggaactcgaa tcccggatag ataaagatgt cagcctcgcc actctgggct gcgatgcggc
     3241 catcgggggt gccccaccct tcggcaagct cagggcaggc tccaagctgg ttttgcttgg
     3301 gtgggccagt gcgtgcagca aattcgagcg tacgcaccgt tgtggtgccc acggccacga
     3361 ttcgccgacc ctcctcgcgt gcgctctgga tgcgttgcgc agcatcggct gagatttcgt
     3421 accactcgcg atgcagtttg tgctcctcaa ccacttccac ccggatcggt tgaaacgtgc
     3481 cgaggcctac atgcagcgtg atctcggcca tctcgattcc gcgctcgcgt atgcgctcaa
     3541 gaatttccgg cgtgaagtgc aggcccgccg tcggcgcggc gacggagccg agcggtttgg
     3601 cgtagatcgt ctggtaacac tcccggtccg acggcgtgtc aggccgatcg atgtagggcg
     3661 gtaagggaat gtggccgatg cgattaagca cgccgaagaa gtcgtccacc ggatcgaagc
     3721 gaagatggcg ctcgccgaaa gttccacggc ccacgatttc ggccgcgagt tcatgtgacg
     3781 attcatcggc ctccccaccg aaataaatcc gctcgcccac cccgagtttg cggccggggt
     3841 gaaccagcgc ttcccactcc ggcggatggg cagagagctg ttttgtcagc agcacttcga
     3901 cccggccgcg aaggaagtct ttcgtcgcgg gattctgggt cgagagccgt tgcgcacgcg
     3961 agccgctacg atgtccgaaa agacgcgcgg gcaacacctt ggtattgtta aaaaccaaca
     4021 aatcgccggg ccgcagcagt tggggaaagt cgcgaaacac gaggtcgtcg aggcgatcgt
     4081 cactgcggct gaggcgcaac agccgcgcag ccgcccggtc ggacagcggt tcctgggcga
     4141 tcagttcctc gggcaggcgg taatcgaaat cggaaaccag cacaaagcga ttatgcctga
     4201 gtgtctcctg cggcccacct ataggaatga aaattaacca cagaggcacg gagatcacag
     4261 agaagattcg aaagaagcca aaaactccgt gtcctccgtg gtaaacgatt ttcgggcga
//
LOCUS       DASRKK010000580         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_148130, whole genome shotgun sequence.
ACCESSION   DASRKK010000580 DASRKK010000000
VERSION     DASRKK010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148130"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFC15_13685"
     CDS             <1..444
                     /locus_tag="VFC15_13685"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11252.1"
                     /translation="IEDLDRQIQQLKAQRKQTAHHIPVKELPESDRFSRLLTERKHFI
                     DTIKLIAYRAETSMASLLRDKLSRADDARALLRQIYDTEVDLIPDLKNKTLTVRLHHL
                     TQAAHDEAVRYLCDQLNATATIFPDTELRLVYQVGTSQIPGDQEF"
     gene            543..1007
                     /locus_tag="VFC15_13690"
     CDS             543..1007
                     /locus_tag="VFC15_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM11253.1"
                     /translation="MPQLQLPIFPAGTTPITAELAFDCQDGKVIYLNGHLPVFQHAQE
                     DLPAFRLFTSQLVVNGTVSQAEIARAFHVPPKTVKRYVKRLREGSSKSFFETPRRRSA
                     SVLKGSVKEQAQALLDAGQAVPEVARELGVLPNTLHKAIRAERLHQPKKKRM"
     gene            1073..>2488
                     /locus_tag="VFC15_13695"
     CDS             1073..>2488
                     /locus_tag="VFC15_13695"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11254.1"
                     /translation="MGYATTRSAERVAAAMGGLASAPIQFETACDVPKGGVLLALPAL
                     LAVGLLRHTAALYQIPKGFYGIASIFLLLALQALARIKSIEQLRYVAPGEWGNLLGLD
                     RIPEVRTLRQKLETLCQEVGRAMRWNTELAKEWIAGQRESELVFYVDGHVRVYHGDLT
                     PLPHHYVARERLCLRATTDYWINAMDGQPFVFVNKEVDPGLLATLRSDLVPWLETNAP
                     ASDELQQRMRDDPLQHRFTLVFDREAYSPEFFAEMKLRRIAILSYHKYPGEDWPVEEF
                     ADCSVRLAGGEEVTLKLAERGTMLANHLWVREVRKLSDGGHQTSILSTNYQADHTLLA
                     VSMFARWSQENFYKYMRQNYNLDRLVEYGAEPVPDPTQTVNPAWRRLESEIRAQSEKW
                     RRQLALFGALELSELAEKEVDDYQRKKGQLRETIEDLDRQIQQLKAQRKQTAHHIPVK
                     ELPESDRFSRLLTERKHFIDTI"
BASE COUNT          557 a          733 c          748 g          450 t
ORIGIN      
        1 atcgaggacc tggatcgcca gatccagcaa ctcaaagcgc agcgcaagca gacggcgcat
       61 cacattcccg tcaaggaact gccggaatcg gatcgcttca gccgtctgct caccgagcgg
      121 aagcacttca tcgacaccat caaactcatc gcctaccggg ccgaaaccag tatggcctcg
      181 ttactgcgcg acaagctaag ccgggccgac gatgcgcgcg cactgttgcg ccagatctac
      241 gacacggagg tggatctgat tccagatctc aagaacaaaa ccttgaccgt gcgactgcat
      301 catctcactc aggccgccca cgatgaagcg gtccgctatc tgtgcgatca actcaacgcc
      361 accgccacca tcttccctga tacggaactc aggctggtct accaagtggg gacatcgcaa
      421 attcctggag atcaggagtt ctgaactcct gatctcgccc cgtgattcct cgggaaacgt
      481 ggaaattgaa ttgaccccaa atcgctcttg cgtcgcgagc ggcgacggcg taagactggg
      541 acatgccgca gttgcagttg ccgatcttcc cggctggtac gactcccatt actgccgagt
      601 tggccttcga ttgtcaggat gggaaagtga tctacctgaa tgggcatctg cccgtgtttc
      661 agcatgcgca ggaggacttg cccgctttcc ggctgttcac cagtcaactg gtggttaacg
      721 gtacggtgag tcaggccgag attgcacgcg cgtttcacgt gccgccgaaa acggtgaagc
      781 gttacgtgaa gcgcttgcgg gaaggcagtt cgaagagctt tttcgagacg ccgaggaggc
      841 gctcggcctc tgtgctcaag gggtcggtga aagagcaagc gcaagctctg ttggacgcgg
      901 ggcaggcagt gccggaagtg gccagggagt tgggagtgtt gcccaacaca ttgcataaag
      961 cgatccgcgc cgaacgcttg caccagccaa aaaaaaagag aatgtaaccg gcgccagcga
     1021 aaccgaggca accagcaaga gccagcgcag cgaaagcgac agcaccgcgg tcatgggcta
     1081 tgccaccacg cgatccgccg agcgggtggc ggcggcgatg ggaggtttgg catcggcgcc
     1141 gatccagttc gaaaccgcgt gcgacgttcc caagggcggc gtgctgctgg cgctgccggc
     1201 actgctggcg gtgggactgt tgcgccacac cgcggcactg taccagatac ccaaaggctt
     1261 ctacggaatc gccagcatat ttctgctctt ggccctgcag gcgttggcac gcattaaatc
     1321 gatcgaacag ttgcgttatg tggcgcccgg ggagtggggc aatctgctgg ggctggaccg
     1381 cattcccgaa gtgcggacct tacggcagaa gttggagacc ttgtgccagg aggtggggcg
     1441 cgccatgcgc tggaatacgg aactggccaa ggagtggatc gccggacaac gcgaaagcga
     1501 gctggtcttc tatgtcgacg gccacgtgcg ggtctatcac ggagatctga cgccgttgcc
     1561 gcatcactac gtagcgcgcg agcgactgtg cctgcgcgcc accacggatt actggatcaa
     1621 tgccatggac ggccagccct tcgtgtttgt gaacaaagaa gtggacccgg gactgctggc
     1681 gacgctgcgc agcgatctgg tgccatggct ggaaaccaac gccccagctt ccgacgaact
     1741 gcagcagcgc atgcgggacg atccgctcca gcaccggttc acgctggtgt tcgaccgcga
     1801 ggcttatagc ccggaattct tcgccgaaat gaaactccgg cgcatcgcca ttctgagcta
     1861 tcacaaatat ccaggagaag actggccggt ggaagagttt gccgattgta gtgtccgcct
     1921 ggcgggcggc gaagaagtga ccctgaaact cgccgagcgg ggcacgatgc tggccaacca
     1981 tctttgggtg cgcgaggtgc gcaagctgtc cgatggtggc caccaaacct ccattctgag
     2041 caccaactac caggccgatc acactctctt ggcagtctcc atgttcgccc gatggtccca
     2101 ggagaacttc tacaaataca tgcggcagaa ctataatttg gaccgcctcg tcgagtatgg
     2161 agccgaaccg gttcctgacc ccacccagac cgttaatccg gcttggcgca ggctcgaatc
     2221 cgagatccgc gcccagagcg aaaaatggcg gcggcaactg gctctgtttg gcgctttgga
     2281 actgtcagaa ctggctgaga aagaggtaga tgattaccaa cgcaaaaagg ggcaactgcg
     2341 agagaccatc gaggacctgg atcgccagat ccagcaactc aaagcgcagc gcaagcagac
     2401 ggcgcatcac attcccgtca aggaactgcc ggaatcggat cgcttcagcc gtctgctcac
     2461 cgagcggaag cacttcatcg acaccatc
//
LOCUS       DASRKK010000581         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_148179, whole genome shotgun sequence.
ACCESSION   DASRKK010000581 DASRKK010000000
VERSION     DASRKK010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148179"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VFC15_13700"
     CDS             <1..1322
                     /locus_tag="VFC15_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZM11255.1"
                     /translation="GGLPGKLADCSERNPDRCEVYLVEGESAGGTAKQGRDRRFQAIL
                     PLKGKILNVEKARYDKMLGHEEIRAMITALGTGIGKDDFDVSKLRYGKIILMTDADVD
                     GSHIRTLLLTFFFRHMQELIKRGNVFIAQPPLYRIKKGKFEQYIKNDTEFVKVMVKRA
                     ADGIVVRYGEGAAMLDGAPLTKFMTTLNDYIGFVDKVNKRIRDEKITETLPKVDLAKR
                     ADFEGDSSHPPKKIIELEKALKAIAKDQGLKSVEKRFEEEHSLWECVFVNSQGAPHII
                     SWELASSPEYRQMMAKYKQIEQYMQPPYYIETVKKESAGSAKEELSDAERTDLEKAEQ
                     KSPKTSKRKAEAETVEKQTVRELFDYVLNEGRKDYTVQRYKGLGEMSSQQLWETTMDP
                     ERRTLLEVKLEDLTETEAIFTTLMGEDVEARRKFIEDNALDVKNLDI"
     gene            1404..2417
                     /locus_tag="VFC15_13705"
     CDS             1404..2417
                     /locus_tag="VFC15_13705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM11256.1"
                     /translation="MNAPKPDERFSRGELRYRIADAINDRLKSIPYLHTTAKSTMKWL
                     ERRRDRKRIAAAQRNVASDAVSSVSGAEPVAGTASLDLLPFPPLEMRRLVGPTELDVY
                     DNPTGALVYPWLPAENYEKVFDFGCGCGRVARQLMLQRPSPTTYVGIDLHAGMIEWCE
                     QNLRRAAPNFNFYHHDVFNVAFNPGNEKPLSAPFPVGDSQFTLVNALSVFTHLTQEQA
                     EYYLRECARVLAPNGVLHASWFLFDKIDHPMMAENYNALYVSYVDPSEAVIFDRTWLR
                     NTARGLGLRIFNVIPPGIRGYQWSVLMTLNPAVEEVDLPPDTAPRGMIDSRRVGRLLP
                     DQD"
     gene            2525..3649
                     /locus_tag="VFC15_13710"
     CDS             2525..3649
                     /locus_tag="VFC15_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11257.1"
                     /translation="MPQIASLVLLLVFVAQCVWFMAHVPITQMESSYIEDGLLHVDRL
                     VNANSEYRSPLIAVLAGFPAQAMGSDRHFARLSDFRFLIRLPFLVAGLMLGASLWYVA
                     RRLYGNLGGYIALGLYCFSPMAITRSSQVQPDIVGAWGAFGLVFTGIAAAHTLYAPRE
                     VVFWNWKRILLMGLSIALCVGAQWSLWVVLLPTLGFMLWVGHVRPKAVIAIFLAACAV
                     GIFLLWGVASFRPFVFADALRNANWIEFSPAQFSGKTVYSLLGLFFLQNGLGPALLLL
                     VSLGTFIFWRRTRYFGTAAPLLTAILLIAVGLTMQHFAGLIFLFIALPFLMLFMAGIA
                     ADLFETRYAVFANAIIVGALVANAIVDISGLLQLGHYASR"
     gene            3690..4430
                     /locus_tag="VFC15_13715"
     CDS             3690..4430
                     /locus_tag="VFC15_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11258.1"
                     /translation="MSSGTQSRSYKRCDTRLLILVGLVMALPLVNAAAQFHGIPPSVT
                     SIQNHFPPYLPNISPSVTSLGPRGYVGPPAFPVYPATSGRFGHGPGNRYGYRNGSGYY
                     GGFVTPYYIPAYDPSYSYDTGGGGPYMYSGPPAEQTLHIVVDLPAAKRSVVEDDEDDP
                     PPAIASKATRNGNATDADAKSIDPTVLVFRDGHQQEVTNYAIMGQTVYVFDNRTQKIA
                     LSDLDVAATIKVNDDRGVDFHVPAHTKS"
     gene            4758..>5293
                     /locus_tag="VFC15_13720"
     CDS             4758..>5293
                     /locus_tag="VFC15_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZM11259.1"
                     /translation="MADDPNQQLPLDPGRGGNIQPINIEEEMRRSYLDYSMSVIIGRA
                     LPDVRDGLKPVHRRILYAMHDMGLLHNRKHVKCAKVVGEVLGKYHPHGDTAVYDAMVR
                     MAQPFSLRYPLVDGQGNFGSVDGDPPAAYRYTESRMTEIAEDLLADIDKDTVDFVANF
                     DDTSVEPVLLPTRIPNLLV"
BASE COUNT         1095 a         1579 c         1540 g         1079 t
ORIGIN      
        1 gcggaggact gccgggcaaa ctggccgatt gctccgagcg taaccccgat cgctgcgaag
       61 tttatctggt cgagggcgaa agcgccggcg gcaccgccaa gcagggacgc gaccgccgct
      121 tccaggccat cctgccgctc aagggaaaga tcctcaacgt cgaaaaagcg cgctacgaca
      181 agatgctggg ccacgaagaa attcgcgcca tgattacggc gctgggcacc ggcatcggca
      241 aagacgactt cgatgtttcg aagctgcgct acggcaagat cattctgatg accgacgccg
      301 acgttgacgg ttcgcacatc cgcacgctgc tgctcacgtt tttctttcgc cacatgcagg
      361 agctgatcaa gcgcggcaac gtgttcatcg cgcagccgcc gctctaccgc atcaagaagg
      421 gcaagttcga gcagtacatc aagaacgaca ccgagttcgt gaaggtgatg gtcaagcgcg
      481 cggccgacgg catcgtggtg cgttacggcg agggcgctgc gatgctcgat ggcgctccgc
      541 tcaccaagtt catgaccacg ctgaatgact acatcggttt cgtggacaaa gtgaacaagc
      601 gcattcgcga cgagaagatt accgagacgc ttccgaaggt cgatctggcg aagcgtgccg
      661 attttgaagg cgatagcagc catccgccga agaagatcat cgaacttgag aaagcgctca
      721 aggcgattgc gaaagatcag ggactcaagt cggtcgagaa gcgctttgaa gaggagcaca
      781 gcctttggga gtgcgtgttc gtaaactcgc aaggcgcgcc gcatatcatc agttgggaac
      841 tggcgtcgtc gccggaatac cgccagatga tggcgaaata caagcagatc gagcagtaca
      901 tgcaaccgcc gtactacatc gagacggtca agaaggaatc cgccggcagc gccaaggaag
      961 aactcagcga tgcggagcgg acggacctgg aaaaagcgga gcagaagagt cccaagactt
     1021 ccaagcgcaa ggccgaggca gagaccgtag agaaacagac agtgcgcgaa ctgtttgact
     1081 atgtgctcaa cgaaggccgc aaggactaca ccgtgcagcg ctataaaggc ctgggcgaga
     1141 tgagttcgca gcagctttgg gagaccacca tggatcccga gcgtcgcact ctgctcgaag
     1201 taaaactcga agacctcacc gagaccgagg ccatcttcac cacgctgatg ggcgaagacg
     1261 tagaagcgcg gcgcaagttc atcgaagaca acgcgctcga tgtgaagaac ctcgacatct
     1321 aacaccgaca aatagcgggc gcatgagatt cccattgcgc tcgcataagg cgtcttctgg
     1381 agggcagggt tctattggat acggtgaatg cgccgaagcc ggatgagcgg ttctcccgcg
     1441 gcgagctacg ttaccgcatt gcagatgcaa tcaacgatcg cctgaagtcg attccctacc
     1501 tccacaccac ggcgaagtcc accatgaagt ggctggagcg ccgcagagac aggaagcgga
     1561 tcgcagcggc gcaacggaac gtggcatccg atgccgtgag ttccgtgagc ggcgcggaac
     1621 cggtggctgg cacagccagt ctcgacttgt tgccgttccc gcctttggag atgcgccggc
     1681 tggtgggccc aaccgaactt gacgtgtacg acaatcccac cggtgcgctg gtgtatccgt
     1741 ggctcccggc agaaaactac gagaaggtat tcgatttcgg atgcggctgt ggacgtgtag
     1801 cgcgacaact gatgctgcaa cggccgagcc caaccaccta tgtcgggatc gatttgcacg
     1861 ctggcatgat cgaatggtgc gagcagaacc tgcgtcgcgc ggcgcccaat ttcaacttct
     1921 atcatcacga cgttttcaat gtcgccttta atcccggcaa tgaaaagccg ctcagcgcac
     1981 ccttccccgt gggtgattcg caatttacgc tggtgaatgc gttgtcggta tttactcacc
     2041 tcacgcagga gcaggcagaa tattacttgc gcgaatgcgc gcgggtgctg gccccgaacg
     2101 gagtgttgca cgcatcatgg ttcctgttcg acaagatcga ccacccgatg atggccgaga
     2161 actacaacgc gctgtacgtg tcgtatgttg acccttccga agccgtgatc ttcgatcgca
     2221 cctggctgcg aaatacggcg cgcggcctgg ggctcaggat ctttaacgtg atcccacccg
     2281 gcatccgagg ataccagtgg tcggtgctga tgacgctcaa cccggcggtg gaggaagtgg
     2341 atctgccgcc tgacactgcg ccgcgcggca tgatcgattc ccggcgcgtc ggtcgccttc
     2401 ttcctgacca ggactagctc ggctggttcc ccgcatagcc ggagcaactt cccgccacgc
     2461 ggttctggcg tgatacaaca aatgcttacg catgcgttcc ttccaaatac tgcctaaagt
     2521 tggattgccc cagatcgcga gcctcgtctt gctgctggtc ttcgtcgcgc agtgtgtctg
     2581 gttcatggca cacgtgccca tcacccagat ggagagcagc tacatcgaag acggattgct
     2641 gcacgtcgat cggctggtta acgccaattc cgaataccgt tcgccgctaa tcgcggtgct
     2701 cgcgggattc cccgcccagg ccatgggcag cgaccgtcac ttcgctcgcc tcagcgattt
     2761 tcgtttcctg atacgcctgc ccttcctggt agcggggctg atgctgggag cgtcgctgtg
     2821 gtacgtggcg cgccggctct acggcaacct cggcggatac atcgcactcg gcctctactg
     2881 cttctctccc atggcgatta cgcgctccag ccaggtgcag cccgacatcg tgggcgcatg
     2941 gggagcgttt ggtctcgtct ttaccggcat cgccgcggcg cacacactct acgctccccg
     3001 cgaagtcgtg ttctggaact ggaaacgcat tctgctgatg ggcctttcca tcgcgctctg
     3061 cgtcggcgcg cagtggtccc tgtgggtggt gctgcttccc actcttggct tcatgctgtg
     3121 ggtgggacac gtgcgtccga aggcggtcat tgcgatcttc ctggcggcat gtgccgtcgg
     3181 gatttttctg ctgtggggtg tagcgagctt tcgcccgttc gtgttcgccg acgctctgcg
     3241 caatgccaac tggatcgagt tttccccggc ccagttctct ggcaagaccg tctattcgtt
     3301 gctgggactg ttctttttgc agaatggcct gggtccggcg cttctgctcc tggtgtcgct
     3361 cggcacattt attttctgga gacgcacccg ctatttcggg acggccgctc cacttttaac
     3421 cgccatcctc ctgattgcag ttggcctcac catgcaacac tttgcaggct tgatcttcct
     3481 attcatcgct ttgccattcc tgatgctgtt catggcggga atcgcggcag acctgttcga
     3541 gactcgctac gcggtctttg ccaatgcgat aattgtcgga gcgctggtgg ccaacgccat
     3601 cgtcgatatc agcggactgc tgcaactcgg acattacgcg agtcgctgac aactttcccg
     3661 catccaaggg gttggcattg gtgggagcca tgtcgagtgg aacccaatcc cgatcgtata
     3721 agcgttgtga cacgcggctg ctgatcctcg tcggcctcgt catggcgctg ccgctggtga
     3781 acgccgcggc gcagttccat ggaattccgc cgtcagtcac ctcgatccaa aatcactttc
     3841 ctccctactt gcccaacatt tcccccagtg tgacttcgct gggaccccgt ggttacgtgg
     3901 gtcctcctgc tttcccggtg tatccggcaa cgtcgggacg gtttgggcat ggccccggaa
     3961 accgctacgg gtacagaaac ggatccggat actacggcgg ctttgtcacg ccgtattaca
     4021 ttccggcgta cgacccctcc tacagttacg acacgggagg tggcggccct tacatgtatt
     4081 cgggacctcc ggccgagcag acgctgcaca tcgtggtaga tctgccggca gcgaagcgct
     4141 ccgtcgtaga ggatgacgaa gacgatccac cgccggccat cgcgtcaaag gcgaccagga
     4201 acggcaatgc gacagacgcc gacgcgaaat ccatcgaccc gacggtgttg gtatttcgcg
     4261 acggccacca gcaggaagtc accaactacg ccatcatggg acagacggtt tacgtattcg
     4321 acaatcgaac tcagaaaatc gctctcagcg acctggatgt agcggcaaca atcaaagtga
     4381 atgacgatcg gggagtagat tttcacgtgc ctgcgcacac caaaagttaa gctttttcag
     4441 tcgctcaaaa tcgccagtcg acacccgttt aggcggcctg cttgaacctg ccttgccatc
     4501 cccctaagtc attgaagaag taacacttat gcctgactgg ctgcgagccg ctcatggtat
     4561 aatcaagcgt gcagttggat cggtccatct cctgaaattg caatgagtta tgcgcatcac
     4621 ggcggtcctg ccgtgagggg tcgcgcggtc agtttgcgcg ctcgtttcgg gacggcttcg
     4681 atccccgcac aacgcagttt ctagaaggga aacaagcttc acgcccgccg catcaggctg
     4741 acggctgata cctcaacatg gctgacgatc caaaccagca actcccactc gaccccggcc
     4801 gcggcggcaa tattcaaccc atcaacattg aagaggagat gcggcgctcg tatctcgact
     4861 attcgatgtc ggtcatcatc gggcgcgcgc tacccgacgt gcgcgacggc ctgaagccgg
     4921 tgcatcgacg cattctgtac gcgatgcacg acatgggcct gctgcacaat cgcaagcacg
     4981 tcaagtgtgc caaggtcgtc ggcgaagttc tgggaaagta ccatccccac ggagataccg
     5041 cggtgtacga cgcgatggtg cgcatggcgc agccgttctc gttgcgctat ccgctggtcg
     5101 atggacaagg caactttggt tcggtcgatg gcgatcctcc ggcggcctac cgatacaccg
     5161 agtcgcgcat gacggaaatc gccgaggacc tgctggccga catcgacaag gacaccgtcg
     5221 atttcgtcgc caacttcgat gacacctcgg tcgagccagt cctgctgccg acgcgcattc
     5281 cgaacttgct ggt
//
LOCUS       DASRKK010000582         5151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_735989, whole genome shotgun sequence.
ACCESSION   DASRKK010000582 DASRKK010000000
VERSION     DASRKK010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735989"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..475)
                     /locus_tag="VFC15_13725"
     CDS             complement(56..475)
                     /locus_tag="VFC15_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZM11260.1"
                     /translation="MSLRKLLLESFNLYTVLDCPSGTFQGAGVKTVVLFFEKGAPTRK
                     TWYYQLDPGRNLGKTNPLNDDDLIEFVKLQETFANSPKSWSVDAKNIDKKTFDLSVKN
                     PNGGEEIVHRSPAEILDEIAALDTEGAEVLSNIKALL"
     gene            889..2355
                     /locus_tag="VFC15_13730"
     CDS             889..2355
                     /locus_tag="VFC15_13730"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZM11261.1"
                     /translation="MSKPTSAAFFSYSRQDSEFAQKLAQDLRSSGASVWLDQLDIRPG
                     QRWDRTVQDAVTSCPRMLVVLSPSSVDSENVMDEVSFALERKKTVIPVLYRDCDIPFR
                     LGRVQYIDFRTDYDRALKELVETLNALEAEQTPPAQVVDKPVDHAVGATQKPRQEPNT
                     AATESDVHRSTVPVASTKEKLEPEAATESVARSARPEPRNAVSAGPTTAEVAKKKWLL
                     PAAIVAGVLIALALVWMASSRKGGQPSPPPSDAVVKISPSDATLAAGETAEFSAQVTG
                     ASDAGVTWSLFGDGDLSDTGVYTAPKDVAAQIKVTVKASSKADPSKYDTASVILKPAR
                     HASKSDASASRATSAQGLASQSKAAPDFSAKQRPPSEPSKNAATAAPTKGGNAEARGL
                     QLAAQASPIEGTDGKRFRFSLSVRTTGLSDIAKVQYDLVYAPNPLLLSSTDPTSNFTA
                     VYEGWGCYRAVNVTLFPKEGSSQQPIKKTFDLCTVLGW"
     gene            complement(2506..2808)
                     /locus_tag="VFC15_13735"
     CDS             complement(2506..2808)
                     /locus_tag="VFC15_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11262.1"
                     /translation="MLNRSAIVVKPRQSFLDRLHTADLTSRNLSLRELAQEPMIYLIP
                     ECDTEEDVREVLQELCEEIFAEQLAGWFTDESTWPVDRSIDVFCRWFDFQHHSVPG"
     gene            complement(2833..3864)
                     /locus_tag="VFC15_13740"
     CDS             complement(2833..3864)
                     /locus_tag="VFC15_13740"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM11263.1"
                     /translation="MLPVFVFFLATLRDPIGARVALQAEVLALRHQLLVLQRRNQKRR
                     LQLSTFDRLLWVWLSRVWADWKSAPRIVKPETVIAWHRKGFRLYWRWKSRPRYGRPPI
                     TTEVKELIRRMSAANPRWGAPRIHGELGKLGIKVSETTVAKYMMRHRHPPSQTWRTFL
                     ANHVKDLVSADFFVVPTATFRLLFVFVILSHDRRRPVHFAVTSHPTAEWTAQQLLQAF
                     PWDMAPRFLLRDRDGNYGKVFRESATALGIEEMLCAPHSPWQNAYAERLIGSIRRECL
                     DHVIVLHEPGLRRVLRSYFEYYTHSRTHLSLAKDAPIPRSIQPPELGRVVELSEVGGL
                     HHRYERRAA"
     gene            complement(3874..4239)
                     /locus_tag="VFC15_13745"
     CDS             complement(3874..4239)
                     /locus_tag="VFC15_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11264.1"
                     /translation="MSVFDRLLWVWQIARDANIRRWEAGILGNLGWLYATQGQKQKAL
                     QSFNEALLLYRDLEDQRGEASILYGMARVNKDLDELSEALSHIESALSIIEKLRTRIT
                     CAYQELILAERIALSEMWQ"
     gene            4494..5048
                     /locus_tag="VFC15_13750"
     CDS             4494..5048
                     /locus_tag="VFC15_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11265.1"
                     /translation="MLSQDEVARWLQAPPHLKTRAVLMTIYAAGLRISEAARLRVPDI
                     DSARMTITIHQGKGNKDRVVMLSPVLLEVLRQYWRRKKPSEWLFPGKNPDQPVGCNAL
                     RRAFHNAVRRAGLTKKVSPHSLRHSFATHLLESGTDLRTIQLLLGHSSLKPTTRYLHV
                     SQKHVRATASPLDSLRLTKERSKK"
BASE COUNT         1154 a         1471 c         1462 g         1064 t
ORIGIN      
        1 gtgtagggca cccgctcgat gcaatcctcg aagggacgtt gttgccacgc gttcttcata
       61 gcagcgcctt aatgttgctc agcacctccg cgccctcggt gtctaacgcg gcgatttcgt
      121 ccaggatttc cgcagggctg cggtgcacga tttcctcgcc accgttggga ttcttcaccg
      181 agaggtcaaa ggtctttttg tcgatgttct tggcgtccac gctccaggac ttgggcgagt
      241 tggcgaaggt ctcttgcaac ttgacgaact cgatgaggtc gtcatcgttt agtgggttcg
      301 tctttccgag attacggccg ggatcgagtt ggtaatacca agtcttccgt gtaggagcgc
      361 ccttctcaaa aaagagcaca acggtcttca cgccagcgcc ctggaacgtg ccactggggc
      421 agtcgagcac tgtgtaaagg ttgaaacttt ccaacaggag ttttcgcagg ctcacagaac
      481 ttcgtccacc atggttcgca tggcacgtag cggatgattc ttccgcaccc gcgttccggc
      541 gacaaataac tgaacatatg gctttgctga tgatccgtgc ctcgcatgct gctttcctag
      601 catgcctctg ctgtggatag cgagtgcttt ttcagcatcc tgctagcgcg tctttcatca
      661 aggcggggcc ttctcgatgc tcattcacag gacatcttta gccccgcccc ttggggcacc
      721 tgaagattca cctgggccac caagacgact aactgtcagc tctcagccat caggcgtcag
      781 cattcgaaac gtgaggatgc tgcgactggt gaggatttcg gggtatattg cgtgtgggtc
      841 gggaatgctc gattcggttc ctggccggat acgcctgaca ggcagactat gtcgaaacca
      901 acatcggcgg cttttttcag ctattcacgc caggactcgg aattcgcgca gaagcttgcg
      961 caggacttga ggtcgtcagg cgcttccgtc tggctggacc aactggatat caggcccggt
     1021 cagcgttggg accgcacggt gcaagacgcg gtgacgagtt gcccgcgaat gttggtggtt
     1081 ctctcgccat cgtcggttga ttccgaaaac gtaatggacg aagtgtcgtt cgcgctggag
     1141 agaaaaaaga ccgtgatccc agtgctgtac cgtgactgcg atatcccttt ccgtttaggc
     1201 cgcgtgcagt acatcgactt caggaccgat tacgaccggg ccttaaaaga actagtcgag
     1261 accctgaacg ccttggaagc cgagcagaca ccgccagcac aggtggtcga taagccggtg
     1321 gaccacgcag tgggcgcaac tcagaaaccg cgccaagaac cgaacaccgc tgcgacagag
     1381 agcgacgtac acaggtcgac tgttccggtg gcgagtacga aagaaaagct tgaaccggaa
     1441 gcagcgacgg agagcgtagc tagatcggcg cgacctgagc cgcgaaacgc ggtgtcggcg
     1501 ggcccaacta ctgcggaagt agctaagaaa aaatggctgc tcccggctgc gatcgttgcg
     1561 ggggtgttga tcgcgctcgc tcttgtatgg atggctagct cgcgtaaggg agggcagcct
     1621 tctccgccgc ccagcgacgc ggtggtgaag atcagtccga gcgatgcgac gctggcagcg
     1681 ggagaaaccg cggagttcag tgcgcaagtt accggtgctt ccgacgccgg tgtgacgtgg
     1741 tccttgtttg gcgacggaga tctatccgac acgggcgttt acactgcgcc gaaggacgta
     1801 gcggcgcaga tcaaggtgac ggtcaaggcc tcgagcaagg cagatcccag caagtatgat
     1861 acggcctcgg tcattcttaa acctgccagg cacgcaagca agtcggacgc ttccgcatcc
     1921 cgcgcaacat ccgctcaggg actggcaagc caaagtaaag cggcgcctga tttttccgcc
     1981 aagcaacgcc cgccatcgga gccaagtaaa aacgccgcca cggctgcacc gaccaaagga
     2041 gggaacgccg aagcccgtgg actacaactg gccgcacagg caagtcctat agaaggtaca
     2101 gacggaaaac gttttcgctt ctcactgtcg gtgaggacga cggggctttc ggatatcgcc
     2161 aaagtgcagt atgaccttgt ctacgctccg aatcctctgt tacttagctc aaccgaccct
     2221 acttccaact tcaccgcggt atatgaagga tggggctgct atcgcgccgt gaacgtaacg
     2281 cttttcccca aggaaggcag cagtcaacag cccataaaga aaacgtttga cctgtgtacg
     2341 gtgcttgggt ggtgaccgta actagaactc atcgcataaa tcattcagaa ccgtatcagg
     2401 gcacgactct ttagtcgtgc cgaacgagac gcaagaaggg ttcgggcttt agagtctgta
     2461 tgagaaccct gccgtccctg ccgggactcg ggctcatttt ccattctacc caggcaccga
     2521 atggtgttgg aaatcaaacc aacggcagaa gacatcaata ctgcggtcca caggccaagt
     2581 gctttcatct gtgaaccaac ccgctaattg ctcggcaaaa atctcttcgc acaattcttg
     2641 caagacttcc cggacgtcct cttccgtgtc acactccggg atcaggtaga tcatcggttc
     2701 ttgagccaac tcacgcaggc tgagattgcg gctggttaga tcggccgtgt gcagccggtc
     2761 cagaaaagac tgtcttggct tcaccacgat tgccgatcga ttcagcataa attaccgaga
     2821 gcctctccac tctcaggcgg cgcgccgttc ataacggtgg tggagtccac cgacctctga
     2881 caactccacc actcttccta actcgggagg ctgtatggag cgtggaatcg gcgcatcctt
     2941 ggcgagggac agatgagtgc gcgagtgggt gtaatactca aagtacgatc tcaacacacg
     3001 ccggagaccc ggctcgtgca ggacaatcac gtgatcaaga cattccctgc gtatggaccc
     3061 gattagccgc tccgcatagg cattctgcca tggcgagtgc ggagcacaaa gcatttcttc
     3121 gattccaagt gctgtagccg attcacgaaa taccttgccg taattgccat cgcgatcgcg
     3181 caacaggaag cgcggagcca tgtcccaggg gaaggcctgc aatagctgct gggctgtcca
     3241 ttctgcggtc ggatggctgg tgactgcaaa gtgtactggc cggcggcggt catgtgagag
     3301 gatcacgaac acaaacagca accgaaatgt agcggtcgga accacgaaga aatcggcgga
     3361 gaccagatcc ttaacgtgat ttgccaagaa cgtacgccac gtctgggaag gtgggtgccg
     3421 gtgccgcatc atgtacttgg cgactgttgt ctcggagact ttgatgccca gcttgcccag
     3481 ttccccgtga atccgcggcg cgccccagcg tgggttggca gcactcatgc gtcgaatcag
     3541 ttccttcacc tcggtcgtta tcggcggacg accataacga ggacgacttt tccaacgcca
     3601 atagagccta aagcctttgc gatgccatgc gatcaccgtt tccggtttta cgatccgagg
     3661 cgccgatttc cagtccgccc aaacgcgcga gagccaaacc cagagaagac gatcaaaggt
     3721 cgacaactgg agtcgccgct tctggttccg gcgctggaga acgagaagtt gatggcgcag
     3781 ggcgaggact tctgcctgaa gtgcaacccg cgcgccgatc ggatcacgaa gcgtggcgag
     3841 gaagaaaacg aagaccggga gcatcaccag agcctattgc cacatctcgc taagtgcaat
     3901 ccgttcagcg agaatgagtt cttggtaggc acaggtaatc ctcgtgcgca atttctcgat
     3961 aatgctcagt gctgactcga tatgagatag cgcttctgac aactcatcga ggtccttgtt
     4021 gactcgcgcc atcccgtaga gaatagacgc ctcaccccgc tggtcctcca ggtcgcgata
     4081 gagaaggagc gcctcattga aagactgaag cgctttctgc ttctgtcctt gggtggcgta
     4141 gagccatcct aaatttccga ggatgcccgc ttcccagcga cggatattcg catcgcgggc
     4201 aatttgccag acccagagaa gacgatcaaa gactgacaac tggtatcgac gtttctggtt
     4261 ccggcgttgg agaacgagaa gttgatggcg caaggcgagg acttctgcct gcagcccaac
     4321 gcgcgcacgg agcgaatcac gaaaagtggc gagcaagaaa accaagaccg ggagcatcac
     4381 ccagagccaa ttgccatatt gcgttaagtg caatcttcta tgtcaagact ctgaagcaac
     4441 ccttcatcct cgaggacatt ccttttcagc gtgagcctca gcagctaccc gagatcctca
     4501 gccaggacga ggtagcgcgg tggttacagg caccaccgca tctgaagacg cgagcggtgc
     4561 tgatgaccat ctacgcggcg ggcttgcgga tttccgaggc cgcccgtttg cgggtgcccg
     4621 acatcgactc ggcacgcatg accatcacca tccatcaagg caagggcaac aaggaccgcg
     4681 tggtgatgtt gtccccggtt ttactcgagg tgctgcgcca gtactggcgg cggaagaaac
     4741 cttcggaatg gctgtttcca ggcaagaacc cggaccaacc cgtcggctgc aacgccttgc
     4801 gcagggcttt tcacaatgcc gtccgacgtg ccggtctgac caagaaggtt tctccgcatt
     4861 cgctgcgcca cagtttcgcc acccatctgc tggaatcagg tacggacctt cgcaccatcc
     4921 agttactgtt gggtcacagc agcttgaaac ccacgactcg ctacctgcac gtttctcaaa
     4981 aacacgtgcg ggctaccgcc agcccactgg attccttgcg cttgaccaag gagcgctcta
     5041 aaaaatgatt cggccacccc tggaggtggc cgacattgtt cgcagctatg gcacagcgtt
     5101 cgtggagcgg catcgccgct ggctgacttt cctgcacctc aaggtcctgc g
//
LOCUS       DASRKK010000583        11596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1126261, whole genome shotgun sequence.
ACCESSION   DASRKK010000583 DASRKK010000000
VERSION     DASRKK010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126261"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFC15_13755"
     CDS             complement(<1..205)
                     /locus_tag="VFC15_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11266.1"
                     /translation="MKLKLAKTLGGLLLALVAGTAAFGHQAATTPSRTVVRAGKLLDV
                     RTGKTLTNQAIVIEDGKIVSVGPD"
     gene            complement(242..2542)
                     /locus_tag="VFC15_13760"
     CDS             complement(242..2542)
                     /locus_tag="VFC15_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZM11267.1"
                     /translation="MKARLFATCCCLFLLTSIPASHAQTPTAPVAPGGQPLTIEAIFA
                     DGGILGRPPEAILWSPDGSKVSYVQRDEGGEHGALYSIDLATVKPTLLVSSDKLSSLA
                     PPASSIKDERQKEAAQRYSIAAYHWSPDSKHLLFDSLGQLWLYSLESGTAVQFTSSAE
                     PVVDPKFSPAGDRVAYVRKHNLYIRDVNHGQEQALTRDGDDNLLNGEVDWVYKEELYS
                     RSNYFWSPDGHSIAFLQMNEKDVPTYPITDWIPTHPTVDMEKYPKAGDPNPLVRIGVV
                     TANGGKLKWLSAGNARDGDLSLGNDPNVLIPRFGWVRDGLLWAMALNRLQNRLDIYFV
                     DVQSGKSKRILIETTDAWVDMHPEVDFELLSTGDRFLWNSWRDGHNHIYLYQFDKQKP
                     LEDEAKLVAQLTHGEWEVERIDGVDENLGLVYFTANEGDWRQANVYAVGLDGKNFHRV
                     SRENGTHAANFGPAKTKYYVDTYSALTTPPRASLCTTDGNCTAFWQSRSVEGYNLLAP
                     KPVQFKAADGVTTLLGTLLLPDGGPMVANGKAPLIVNPYGGPGVQHVRDSWGGDSYLF
                     DQVLARQGFAILHVDNRGMANRGKAFVLPVKHNLGEIEFNDQMASVKQALQQYPQLDA
                     SRMGFWGWSYGGYFTLYTLEKTDIFKAGVAVAPVTDWRNYDSIYTERYMGLPTDNEEG
                     YRKSSPVNFPADLHGSLLEVHGTSDDNVHMQNTIQMVNAFINSGKQFQLMMYPRKTHG
                     IAGKTARTHLFHLIEDHFEKNLSPGK"
     gene            2677..3483
                     /locus_tag="VFC15_13765"
     CDS             2677..3483
                     /locus_tag="VFC15_13765"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HZM11268.1"
                     /translation="MTSDLQNLIELQQIDSQIAALRAEIAALPKHVAQIEAKLAGSKA
                     KVEATEAAAKADEGARRKHESDIQDQQQKISKYRDQSLNVKTNLEYKALMEEIKFAEQ
                     KIAASEDKILEIMVAADSRKESLKKLEAELKAVTAENDREKEEARQRTAADEAALAEL
                     TTRRNGSRTGVSEEALRHYDHVLKVRGTALAAVYDNEQCGACNVMLRPQVFQEVMTNQ
                     QFMTCDYCKRILYYVPPPPKPEDPKAAKADAAPLEPDTGEESASSETVSQ"
     gene            3517..4755
                     /locus_tag="VFC15_13770"
     CDS             3517..4755
                     /locus_tag="VFC15_13770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZM11269.1"
                     /translation="MSTQARFAEGLLSSHEFIPVITVSARGATRLKAGHLWVYRSDLA
                     KTGDIRAGALVHVQDERGRKLGSALYSSSSQIAIRLLTSAVIGESRLLPLLRERIDGA
                     AQFRKQFARDTDSYRVIFSEADLLPGLIVDKYNDVLSMQVVTQAFDREDLRTVVTDAM
                     AENFPDSSLFERVDPHIRQLEQLPARESQLIQGDRSSTIFTLNGVRFHFDALGGQKTG
                     AFLDQRENYAAAARYAHGEALDCFTYQGGFALHLARVYSKVTAVDSSRPALEVADQNE
                     QLNRSGHICGEIEWVEANAFDLLKDYSTAGQQYDTIVLDPPAFAKTKRVLETAIRGYK
                     ELNLRALKMLRPGGVLVTCSCSFHVSEGEFLDMLGAAAADAGRRVRILEKRSAAIDHP
                     MLSGVPETAYLKCIICQVIE"
     gene            5064..7700
                     /gene="clpB"
                     /locus_tag="VFC15_13775"
     CDS             5064..7700
                     /gene="clpB"
                     /locus_tag="VFC15_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZM11270.1"
                     /translation="MAIRWDKLTVKAQESVQRANDIASEHGNPELQPVHILAALLEDH
                     EGIVAPLLERVGLQVPAVQAEAMAQIERLPKVSGGGAAQASLSPAANQLLENAFKEAA
                     NFKDEFVSTEHLLLAMTKLKRDAAQEILARAGATYDNILRGLTSVRGNQKVTDQNPEA
                     KYQALERYARDLTELARRGKLDPVIGRDEEVRRVVQVLSRRTKNNPVLIGEPGVGKTA
                     IVEGLAQRIISGDVPEQLKNKRVVSLDLGAMLAGAKYRGEFEDRLKAVLKEIEQSEGK
                     VILFIDELHTLVGAGAAEGAIDASNMLKPPLARGELRAIGATTLNEYRKYIEKDAALE
                     RRFQIVFVGEPSVEDTIAILRGLREKYEVHHGVRIKDSAIVAAATLSHRYISDRFLPD
                     KAIDLIDEAAASLRIQIDSMPTEIDQLERRATQLEIERQALKREDDRNSRERLAAVEK
                     ELAQVKEQSNALKARWKQEKEAISRVREMKEKLEQLKLEIQDVTRKGDFNRAAQLQYG
                     ELPKMEAEIKNLSSQMDGRQSRMLKEEVDEEDVAKIVSKWTGIPVSKMLEGEVKKLVT
                     MEEGLRHRVIGQDAALERVANAIRRSRAGLSDPRRPIGSFIFLGPTGVGKTELARALA
                     EFLFDDEHAMIRIDLSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYAVVLFD
                     EIEKAHPDVFNIFLQIMDDGRLTDGKGRHIDFKNTIVIMTSNIGSSYLQQAGAAPDKQ
                     EEAHEQVMDALRTHFKPEFLNRVDDIIFFNPLGKEQLTKIVDLRLEDLRKLLADRKIT
                     LQLTPKAKELIFTEGYDPNYGARPLKRAIQKLVQDPLAMKILDGEVLRGDRVKVDADP
                     KAKKLTFKVTQREVESEAVAAK"
     gene            7811..8434
                     /locus_tag="VFC15_13780"
     CDS             7811..8434
                     /locus_tag="VFC15_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11271.1"
                     /translation="MPDPRPCRRTPRKQLPQPDVEPDALDHLTVIRQTMERSATFTAV
                     SGWGYCAMGVTAMVAAAIAMRQPTTEAWLAVWLAEALTAVTLALVAMHWKAARLGTHL
                     MSVSGRRLFVGLLPALFAGGVMTVALLRAGDPRQIPGLWLLLYGVAVMQAGAFSVRTI
                     PVMGAVFVLAGAIALPMPWWWANVTLAAGFGLVHIGFGLFIARRHGG"
     gene            8469..8804
                     /locus_tag="VFC15_13785"
     CDS             8469..8804
                     /locus_tag="VFC15_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZM11272.1"
                     /translation="MKHIASRRAPAGIEAVKLDAVIHERARLGIISALAVNDELSFND
                     LKKLLEITDGNLSVHARKLEDAGYLKCSKSFVGRQPRTTFRLTAAGRRALEDYLKQME
                     SVIRSARKS"
     gene            8836..9123
                     /locus_tag="VFC15_13790"
     CDS             8836..9123
                     /locus_tag="VFC15_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11273.1"
                     /translation="MDGQQINRFSGRVILILSLAALLTVLIGYTQPPQPDEGALAHIF
                     QLTIAAVVLMVFLFFATADWRQPFKSARPLALPAAALVLAFGALYYLEHFR"
     gene            9217..9888
                     /locus_tag="VFC15_13795"
     CDS             9217..9888
                     /locus_tag="VFC15_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11274.1"
                     /translation="MYTNGNFESHLAALLFLGTLGLLILLSAATVAAFFWRRAWVRYP
                     LLAIAVLLAGYALLLAVFSLASYDRTVARGDEKFFCALDCHIAYSVQNVDRTKAIGDT
                     TARGEFYVVTLRAHFDERTIAPWRGDSPLNPDPPALTLVDAGGRSYPVSPTGQKAWEA
                     AHPQTHSITDPLRPGESYETTWVFDLPAGAQSVRMLAGWNGFPSYVLIGDEASLGHRK
                     TYFAL"
     gene            complement(10298..11113)
                     /locus_tag="VFC15_13800"
     CDS             complement(10298..11113)
                     /locus_tag="VFC15_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HZM11275.1"
                     /translation="MMTKNSRYLLVRDKPDSNDYLYPPKSEHVRKRLPERVDLRSLCP
                     PVQNQGKLETCTAHAVAGALHYEHRLQSQRAIRPSRLFIYYNERPIAHEKRLRPSVNL
                     RDALKVVAKHGACPEHLHPYNVLPRATIKKPSPAAYRAAAKHKIAAYYRISHGSLTPE
                     QFLHHLKSCLADRHPVVFGFRIHPSFVPPKTEEWEAGMMPIPHKRKDRGHGGHAVMAV
                     GYDDRKQAVLVRNSWGKGFGIKGYFWMPYSLISDSGFAHDFWTTRGIVGEAKK"
BASE COUNT         2437 a         3249 c         3540 g         2370 t
ORIGIN      
        1 agtcaggacc gacgctcacg atcttgccgt cctcaatgac tatggcctga ttggtcaggg
       61 tcttgccggt gcggacatcg agaagttttc cggcgcgtac gacggtgcgt gagggagttg
      121 tggcagcctg gtgtccgaat gcagcggtgc cagctacgag agcaagaagc agaccaccga
      181 gagttttcgc gagtttcaat ttcattcgcc ttccggtttc ttgaagtatt tatttcgccg
      241 gctacttccc gggcgacagg ttcttctcga aatgatcttc aatcaggtgg aacaggtgcg
      301 tgcgcgcggt tttgccagca atcccgtgtg tcttacgggg atacatcatt aactggaatt
      361 gtttgccgga attgatgaac gcgttgacca tctggatggt gttctgcatg tgcacgttgt
      421 cgtcgctggt gccgtgaact tccaacaagc tgccgtgcaa gtcagccgga aagttcaccg
      481 gagaactctt gcgatagccc tcttcattgt cggtgggcag tcccatgtag cgttcggtgt
      541 agatggaatc atagttgcgc cagtcggtca ccggtgcaac ggccactccg gccttaaaga
      601 tgtcggtctt ctccagtgtg tagagggtga agtagcctcc gtagctccag ccccagaagc
      661 ccatgcggga cgcgtcgagt tgagggtact gctgcaaggc ttgtttgacg gaagccatct
      721 ggtcgttgaa ttcaatttct cccaagttgt gtttgacggg gaggacaaaa gctttgccgc
      781 ggttcgccat tccgcggttg tcaacgtgca ggatggcgaa tccctggcgg gccaggacct
      841 gatcgaagag ataggagtcg ccgccccagg aatcgcgaac gtgttgtacg cccggcccac
      901 cgtatgggtt cacgatgagg ggagctttgc catttgccac catcggtccg ccgtcgggca
      961 gcagcagcgt acccagcagc gtcgtgactc catcagcggc cttgaactgc actggcttcg
     1021 gagcgagtaa gttgtatccc tcgacgctgc gcgactgcca aaacgccgtg cagttgccat
     1081 cggtcgtgca cagcgacgca cgcggaggag tggtcagagc tgagtaggta tcgacgtaat
     1141 actttgtttt cgcaggcccg aaattggccg cgtgcgttcc gttctcccgg gagacgcgat
     1201 gaaaattttt gccatctaat cccacggcat agacgttggc ctggcgccag tcgccctcat
     1261 tggcggtgaa gtaaaccagg cccaggtttt cgtcgacgcc gtcaattctc tccacttccc
     1321 attcgccgtg cgtcaattgc gccaccagct tggcttcatc ctcgagtggt ttctgtttat
     1381 cgaactgata gagatagatg tgattgtggc cgtcgcgcca actgttccac aggaagcggt
     1441 cgcccgtgga taacaactcg aaatccactt ccggatgcat gtcgacccag gcgtcggtgg
     1501 tctcgatcag aatgcgtttt gatttaccgc tctgcacatc cacgaaatag atgtcgagcc
     1561 ggttctgcag gcggttcagg gccatggccc acagcagtcc gtcgcgcacc cacccgaagc
     1621 gcgggatcag gacgttgggg tcattgccta acgacaggtc tccgtcgcgc gcgttgcccg
     1681 ccgaaagcca cttcagcttg ccgccattcg cggtgaccac tccgatacga accagaggat
     1741 tcggatcgcc cgccttggga tatttctcca tgtcgacggt gggatgtgtc gggatccagt
     1801 cggtgatggg ataggtcggc acgtctttct cgttcatctg aagaaatgca atcgaatggc
     1861 cgtcgggaga ccagaaataa ttgctgcgcg aataaagttc ctctttgtag acccagtcaa
     1921 cctcgccatt caggaggttg tcgtcgccgt cccgggtgag ggcctgctcc tggccatggt
     1981 tcacgtctcg aatgtacagg ttgtgcttgc gaacataggc aacgcgatcg ccagcgggcg
     2041 aaaattttgg atctaccacg ggctcggcgc tcgacgtgaa ttgaacggcg gtcccactct
     2101 cgagactgta gagccagagt tgaccaagcg aatcaaacag caggtgcttg gaatccggcg
     2161 accagtggta ggccgcgatc gagtaccgct gcgcggcttc cttttggcgc tcgtccttga
     2221 tggaactggc aggaggcgcg agcgacgaca gcttatcgct ggacaccagt agcgttggtt
     2281 tcaccgtggc gaggtcgatg gagtagagcg ctccgtgctc gccgccttca tcgcgttgaa
     2341 cgtaggaaac cttagagccg tccgggctcc ataggatggc ctcgggcggg cgccccagaa
     2401 tacctccgtc ggcgaagatc gcctcaatcg tgagcggctg tccgcccggg gcaacagggg
     2461 cggttggggt ttgggcgtgc gatgcaggta ttgaagtcag cagaaaaaga cagcagcaag
     2521 ttgcaaatag acgagctttc acggacgctc cgagcagtaa gtttcgttgg gcagcagtgt
     2581 atatcacgag gttggccgca ggtggagaga tggcgaagac gaaatcagcc tgcgattcct
     2641 gcgtgctaga atccagtcag gttttcccag ggtctgatga cttccgatct tcaaaatctc
     2701 atcgagctgc agcagattga ttcgcaaatc gccgcgctga gggctgaaat cgccgcgttg
     2761 cccaagcacg tggcacagat cgaagccaaa ctcgccggtt ccaaggcaaa ggtcgaagcc
     2821 acggaggccg ccgccaaggc cgacgaaggc gcccgccgga aacacgaatc ggatatccag
     2881 gaccagcagc agaaaatcag caagtaccga gaccagtcgc tcaacgtcaa gactaatctt
     2941 gaatacaagg cgttgatgga agagatcaag ttcgcggagc agaagatcgc cgcctccgag
     3001 gacaagatcc tggaaatcat ggttgccgcc gattcgcgca aggaatcgct gaagaagttg
     3061 gaagccgagc taaaggcggt cacggccgag aacgatcgcg aaaaggaaga agcgcgccaa
     3121 cgcaccgcag cagacgaagc agccttggct gagctgacga cgcgccgaaa cggatcgcgt
     3181 accggcgtca gcgaagaggc gctgcgccat tacgatcacg tgctcaaggt gcgggggact
     3241 gcgctggcgg ctgtttacga caacgagcag tgtggagcgt gcaacgtcat gctgcgtccc
     3301 caggtatttc aagaggtcat gaccaaccag cagttcatga cctgcgatta ctgcaagcgc
     3361 attttgtatt acgttccgcc accacccaag ccggaagacc cgaaagctgc caaagctgat
     3421 gctgcgccgc ttgagccgga caccggagaa gagtccgctt cgagcgaaac tgtttcccag
     3481 taaatcctgc ctgcttctta tccgcaaaca tttaagatga gcactcaagc tcgatttgca
     3541 gagggtctcc tgtcttctca cgaattcatt ccagttatca cggtcagcgc tcgcggcgcc
     3601 actcgcctca aggcgggaca cctctgggtg tatcgctccg acctcgccaa aacaggcgac
     3661 atccgagctg gggcgttggt gcatgttcag gatgagcgcg gccgcaagct tggttcggca
     3721 ctttacagca gctcgtcgca aatcgcgatc cgcctcctga cgtctgccgt gatcggcgaa
     3781 agccgactgc tgccactgct gcgcgagcga atcgacggcg cagcgcagtt tcgtaagcag
     3841 ttcgctcgcg acaccgattc ctaccgcgtg atcttcagcg aggccgacct cctgcccggc
     3901 ctgatcgtgg acaagtacaa cgatgtgctc tcgatgcaag tagtgaccca ggccttcgac
     3961 cgcgaggatc tccgcaccgt ggtgacggac gcgatggcgg agaattttcc cgatagcagc
     4021 ctgttcgaac gggtggaccc gcacatccgc cagttggagc aacttccggc gcgcgaatcg
     4081 cagttgattc aaggcgaccg gtcgagcacg atctttacct tgaacggcgt ccgcttccac
     4141 ttcgacgccc tcggcggcca gaagacggga gccttcctcg accagcgcga gaactacgcc
     4201 gccgctgctc gctacgccca cggcgaggcc ctggactgct ttacctacca gggtggcttc
     4261 gccctgcatc tggcgcgagt gtacagcaag gtgacggcag tcgattcttc gcgccccgcc
     4321 ctggaagttg ccgaccaaaa cgaacaactc aaccgctccg gccacatctg cggcgagatc
     4381 gagtgggttg aggccaatgc cttcgacttg ctcaaggact actccaccgc cggccagcaa
     4441 tacgacacca tcgtcctcga ccctccggcc ttcgccaaga ccaagcgcgt tctggagact
     4501 gccatccgtg gctacaaaga gttgaatctg cgtgccctga agatgcttcg cccgggagga
     4561 gtgctggtca cctgctcctg ctcgtttcac gtcagtgagg gcgagttcct ggacatgctg
     4621 ggagctgcag ccgccgatgc cgggcgaagg gtgaggatat tggaaaaacg gagcgctgcc
     4681 atcgaccatc ccatgttgtc aggcgttcct gagaccgcct acctgaagtg cataatctgc
     4741 caagttattg aataaagtac atttaaacgc attatcgagc ccgaagtgca tcttcagttc
     4801 ttaatagttg atagtaatac gataagattt tatgcttcgc tcactattat atatgcatct
     4861 aaacgtgaat gaggatagtc gccgggctgc gtcagcacaa ctcggccatt gaactcggtc
     4921 agggaacggt catgatcgcg ccctcgtctc tacatccgcc gatggctccg gcgaaaatat
     4981 tccggttcac tctgattgat aggttcatac aattcataat tggtggcctt ggggccgcct
     5041 gcaatcgtta ataggagtac gtaatggcaa ttcgatggga caaactaacc gtgaaggcgc
     5101 aggagtcggt gcagcgcgcg aatgatattg ccagcgaaca cggcaatccc gagttgcagc
     5161 ctgtccacat tctggctgct ctgctggaag atcatgaggg catcgtcgct ccgctcctgg
     5221 aacgcgtggg cctgcaggtc ccggcggtcc aggccgaggc catggcgcaa atcgagcgct
     5281 tgcccaaagt gtcgggcgga ggcgcggcgc aggcgtcgtt gtcgcccgct gccaaccagt
     5341 tgctggagaa cgccttcaaa gaggcggcca atttcaagga cgagttcgtc tccactgagc
     5401 acttactgct cgcaatgacc aagctcaagc gcgatgcggc gcaggagatc ctggcccgcg
     5461 cgggcgcgac ctacgacaac attctgcgcg gactgacttc cgttcgtggc aaccaaaaag
     5521 tgaccgacca gaaccccgag gcgaagtacc aggcgctgga gcgctacgct cgcgatctga
     5581 cggaactggc gcgtcgcggc aaactcgatc ccgtgattgg gcgggacgaa gaagtgcgtc
     5641 gcgtggtgca ggtgctgtcg cgtcgtacca agaacaaccc cgtgctgatc ggcgagcccg
     5701 gagttggcaa gacggccatc gtcgagggcc tggcgcagcg catcatttcc ggcgacgtgc
     5761 ccgagcagtt gaagaacaaa cgcgtcgttt cgctcgactt gggagccatg ctcgcaggcg
     5821 caaagtatcg cggcgagttt gaagaccgcc tcaaagctgt gctcaaggaa atcgagcagt
     5881 ccgagggaaa ggtcatcctg ttcattgacg aactgcacac gctggtgggt gccggagctg
     5941 cggagggtgc gatcgacgcc tccaacatgc tgaagccgcc tctggcgcgc ggtgaattga
     6001 gggccattgg cgcaactacg ctcaacgaat atcgcaagta catcgagaaa gatgccgcgc
     6061 tggagcgtcg tttccagatc gttttcgtgg gcgagcccag cgtggaagac accatcgcca
     6121 ttctgcgcgg actgcgtgag aaatacgaag tgcatcacgg cgtccgcatc aaggactcgg
     6181 cgattgtggc tgcggcaacc ttgtctcatc gctacatttc cgaccgcttc ctgccggaca
     6241 aggccatcga cttaatcgat gaggccgcag cgtcgttgcg catccagatc gactcgatgc
     6301 ctacggagat cgatcaactt gagcgtcgcg ccacgcagct cgaaatcgaa cggcaagccc
     6361 tgaagcgcga ggacgatcgc aactcgcgcg agcggctggc ggccgtcgag aaagaactcg
     6421 cgcaggtgaa ggagcagtcc aatgctctga aagcgcgctg gaagcaggag aaagaagcga
     6481 tctctcgtgt ccgcgagatg aaggaaaagc ttgaacagct gaagctcgag atccaggacg
     6541 tcacccgcaa aggcgacttc aatcgtgcgg cgcaacttca gtacggtgag ttgcccaaga
     6601 tggaggccga gatcaagaac ctcagcagcc agatggacgg tagacagtcg cgcatgttga
     6661 aggaagaggt cgatgaggaa gacgttgcca aaatcgtcag caagtggacc ggcattccgg
     6721 tttccaagat gctcgagggc gaagtcaaga agctggtcac catggaagag ggcttgcgcc
     6781 atcgtgtcat cggacaggac gcagcgctgg agcgcgtggc caacgccatc cggcgttcgc
     6841 gcgctggact tagcgatccg cgacgtccca tcggctcgtt catcttcctg ggaccaacgg
     6901 gtgtcggtaa gacagaactg gcaagagcgc tggcggagtt tctgttcgac gacgagcacg
     6961 ccatgatccg catcgacctg tcggaataca tggagaagca ctcggtgtcg cgactgatcg
     7021 gcgcgcctcc gggatacgtg ggttatgacg aaggcggtca actgactgag gcggtgcggc
     7081 gacgtccgta tgcggtggtg ctgtttgacg aaatcgagaa ggcgcatccc gacgtgttca
     7141 acatcttcct acagatcatg gacgacggcc gtctcaccga cggcaaggga cgccatatcg
     7201 acttcaagaa caccatcgtc atcatgacgt cgaacatcgg ctcgagctat ttgcagcagg
     7261 ctggtgctgc tcccgataag caggaggagg cgcacgaaca ggtgatggac gctctgcgaa
     7321 cgcacttcaa gcccgagttt ctgaaccgcg tggatgacat catcttcttc aacccgcttg
     7381 gcaaggagca gttgacaaag attgtggacc tgcggctgga agacctgcgc aagttgctgg
     7441 ccgaccgcaa gatcacgctg cagctgacgc cgaaggcgaa ggaattgatc ttcaccgaag
     7501 gctacgatcc gaactatggt gcgcgtccgc tgaagcgggc tattcagaag ctggttcagg
     7561 acccattggc gatgaagatc ctcgatggcg aagtgctgcg tggcgaccgc gtaaaggtgg
     7621 acgcggaccc caaagccaag aagctgacgt tcaaggtcac acagcgcgag gtcgaaagcg
     7681 aagcggtcgc ggcaaagtaa ttcgcgatgt tatgaacggc ggcacagtcg aagggcggtg
     7741 cctttttgtt gctagccttt gcaacataaa gtacttgaca aacataattg gagcggataa
     7801 tgtggctttc gtgcctgacc cacgtccatg ccggcgcact ccccggaagc aactaccgca
     7861 gcctgatgtc gagcccgatg cgctggatca cctgactgtc atccgacaga ccatggaacg
     7921 cagcgcgacg ttcaccgctg tttccggctg gggctattgc gccatgggag tcacagctat
     7981 ggtcgcagcc gcaatcgcca tgcgacaacc caccacggaa gcctggctgg cggtgtggct
     8041 ggccgaggca ctaacggcag tcacgctggc actggttgcg atgcactgga aggccgcgag
     8101 gctcggcacc catctcatgt cagtttcggg gcgccggctg ttcgtcggat tgctccctgc
     8161 gctcttcgcc ggcggagtca tgacggtggc tttgctgcgc gcgggagatc cgcggcagat
     8221 tcccggtctc tggttgctgt tgtatggagt ggccgtcatg caggcgggag cattttcggt
     8281 gcgcacgatt ccggtcatgg gagcggtgtt cgtgctggcc ggagccatcg cgctgcccat
     8341 gccgtggtgg tgggccaatg tcacgctggc cgctggattc gggctggtgc acattgggtt
     8401 cggcctgttc atcgcaagga ggcacggtgg ttaagcacgc aagtcaagtt gagcgggatg
     8461 tattgcctgt gaagcacatt gcaagtcgtc gtgctccagc ggggatcgaa gcggtgaagc
     8521 tcgacgccgt gattcatgag cgcgcgcggc tggggattat cagcgcgctg gcggtaaacg
     8581 acgagctcag cttcaacgat ttgaagaagc tactggagat caccgacggg aacctcagcg
     8641 tccacgctcg caagttggaa gatgcgggct acctgaagtg ttcgaagtcg ttcgtagggc
     8701 gccagccgcg caccacgttc cgattgaccg cagcgggcag gcgcgcgctg gaagattatc
     8761 tgaagcagat ggagtcggtg atccggtcgg cgcgcaagag ctaggcgcgc ttgaatccgg
     8821 atggaaagtg acgctatgga tggccagcaa atcaaccgat tcagcggcag agtcatactc
     8881 atcctgtctc tagcggcgtt gctgacagtc ctaatcgggt acacccagcc gcctcagccg
     8941 gacgaaggcg cattggcgca tatctttcag ctaacgatcg cggcggtggt actgatggtc
     9001 tttctcttct tcgccactgc ggattggagg cagcctttca aaagcgcgcg acctctggca
     9061 ttgccagcgg ccgccttggt tctcgcgttt ggagcgttgt attacctcga gcacttccga
     9121 tgaaccctga aattcaatgt ggcacaccgc cccaaagccc ccttttcttt gttccagtac
     9181 tttgtaatgc aaagtactta acagaaagat cgactcatgt acaccaacgg caacttcgaa
     9241 tcgcacctgg cggcgctgct gttcctgggg acgctgggtc tgctgatact gctcagtgcg
     9301 gccaccgtgg cggcgttctt ctggcgacgc gcgtgggtcc gatatccgct gctcgcgatt
     9361 gccgttctgc ttgctggcta cgccctgttg ctggcggtgt tctcgctcgc cagctacgac
     9421 cgcactgtgg cgcgaggcga cgagaagttt ttctgcgcgc tcgactgcca catcgcctac
     9481 tcggtgcaga acgtggaccg gacgaaggcg atcggcgaca caaccgctcg cggcgagttc
     9541 tacgtggtaa cgctgagggc gcacttcgac gagcggacca tcgcgccgtg gcgtggggac
     9601 agtcccctga atcctgaccc gccggcgctg acgctggttg atgctggcgg gcgttcctac
     9661 ccggtttccc cgactgggca gaaggcgtgg gaggcggccc atccgcagac ccattcgatt
     9721 acggacccgc ttcgtccggg cgagtcgtat gagacgacct gggtgtttga cctaccggcg
     9781 ggggcccagt ccgtacggat gcttgccggt tggaacgggt tcccgtcgta cgtgcttatc
     9841 ggggatgagg ccagcctggg acacaggaag acgtacttcg cactgtgaca attcttaaaa
     9901 aaacagaatt cacgactgtt tagaatcaat ggtttggcag gaaattgttc gcaagagttt
     9961 gattcttata taagttatgc gtatccatca gtgcgcgtag gggttaggtt gggccctttt
    10021 cgcctaatgc tcgcgcagac gaaattttcg cgccacagtt tacgcagcgg cggcaagtgt
    10081 tacaggcgta gttgccgagc aggcgagcga gttatcaaag agccaatgag atccgcggtc
    10141 tgggagcgga acgggtaggg acacgtatcc cttccccgtc tcgagatata gatcgggggt
    10201 ggcagtaagc gctagagaaa tcgggagggc gaagaaacta aagtaaccac gtggctcggg
    10261 ctaggcggag cgcgaagctg gggcatccgc ctgtgtctta cttcttcgcc tcgccaacga
    10321 tgccgcgtgt cgtccagaag tcgtgggcga atccggaatc gctgatgaga ctgtagggca
    10381 tccagaagta acccttgatg ccaaaccctt ttccccacga gttccttacc agcaccgcct
    10441 gtttgcggtc gtcatagccg accgccatga cggcgtgacc gccgtgtccg cggtcttttc
    10501 tcttgtgggg aatgggcatc atcccagcct cccactcttc ggtcttgggc ggcacgaaac
    10561 tgggatgaat cctgaaaccg aacacgacgg ggtgccggtc ggcgaggcag ctcttcaaat
    10621 ggtgcaagaa ctgttccggc gtcaacgatc cgtgcgaaat acggtagtac gcggcaattt
    10681 tatgcttggc agcggcccgg taggcggcag gggacggttt ctttatggtg gcgcgcggca
    10741 gcacgttata gggatggagg tgttccgggc aggcgccgtg tttcgccact actttcagcg
    10801 catcgcgcaa attgacggaa ggtctcagcc gcttctcatg agcgatgggc cgctcattgt
    10861 agtaaatgaa gagccgcgac ggacggatgg cgcgctggct ctgcaaccgg tgttcgtagt
    10921 gaagcgcacc ggccacggcg tgcgccgtgc acgtctccag ctttccctgg ttttgcacgg
    10981 ggggacaaag gctccgcagg tccacgcgct ccggcaggcg tttgcgaaca tgctccgatt
    11041 ttggaggata gaggtaatcg ttggaatccg gtttgtctcg aaccagcagg tagcgcgaat
    11101 ttttagtcat catagggccc ttacacctga cagggattct taactgcatt tcggtgttgc
    11161 ggcaatatac accagattcg cccgagtcca cacaatcgcc aagacggacg gggcactcgg
    11221 cgactaataa agaagtttcc agtttcaact ttcgagtgag gtggcagcaa gacgcctcaa
    11281 tttagactcc gcgccgtctg aagtggcagt tgagttggcg gtcctgaatg ccggattgcc
    11341 agtcaagcca ctgagcgacc catatggcca cgagaccgag gattgctagc tggaacaccc
    11401 acttaggcat aacggagaga gtataacgct taacaggtgt ggtttaatca agagaacaac
    11461 tacgatgcca accgcggccg tttggcgtgt ctgcgttgac agacggcgcg tgactcgttt
    11521 attcttaggt ctttggcagt tctgcgtttg cccgcctggt ggttccctgc gcgtccagcg
    11581 tcacgaactc agcggc
//
LOCUS       DASRKK010000584         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_833068, whole genome shotgun sequence.
ACCESSION   DASRKK010000584 DASRKK010000000
VERSION     DASRKK010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833068"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1111
                     /gene="truB"
                     /locus_tag="VFC15_13805"
     CDS             227..1111
                     /gene="truB"
                     /locus_tag="VFC15_13805"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZM11276.1"
                     /translation="MPLNGVIVVDKPAGWTSHDVVAKARRILGERSIGHLGTLDPMAT
                     GVLPLVVGRMTRLCQFYSSSEKAYQGTIRLGVATDTYDCDGDPVGPAQDVNVTLEEIR
                     AVVFGFVGLIQQTPPSFSAKKIKGVPAYKLARKKQEVELQPVEVEVKEFTVLGLNGDL
                     VDFRCRVSSGTYVRSLAHELGQKLGIGAHLASLHRTAVAEFAIEESHTLDEIGEAASQ
                     GRIEELLVHPRRVLPEIPSVTASDEDIAKIRHGRTVNLPEMSRSRFVKVFAGQSELIC
                     IASRVAGTLFHPKVVLCS"
     gene            1154..1489
                     /locus_tag="VFC15_13810"
     CDS             1154..1489
                     /locus_tag="VFC15_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM11277.1"
                     /translation="MPADETPVLQLEIERRPEANIVHCTGKVVIDSWAMFTVAVRALI
                     PEGKAILVDLANVSLVDSVGIGTFVSVWASARKGGCELKFINLNEKIHDLFEITRLHG
                     LFGGDVAGA"
     gene            1721..1948
                     /locus_tag="VFC15_13815"
     CDS             1721..1948
                     /locus_tag="VFC15_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZM11278.1"
                     /translation="MEVNIHEAKTHLSKLLERVASGEEVIIAKAGKPVARLVPIKPKR
                     ALRPLGLARGEFVVPDDFDAPMPDFEEDFYR"
     gene            1945..2331
                     /locus_tag="VFC15_13820"
     CDS             1945..2331
                     /locus_tag="VFC15_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM11279.1"
                     /translation="MKVLLDTHIFLWAISDPDRLSSRTQQIVKDARNDVLLSVVSAWE
                     IAVKSSLGKLDLPRPAMAFVQRQLARHRIGLLPLSLSHLSALESLPWHHRDPFDRMLV
                     AQCREEGASLITVDKQLHRYEVEILS"
     gene            complement(2351..>2636)
                     /locus_tag="VFC15_13825"
     CDS             complement(2351..>2636)
                     /locus_tag="VFC15_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11280.1"
                     /translation="IYLAGFELQTFERYPNSIGVVRDGCIALLRATPVGLQMIGTPGW
                     RMGEVLGVLVEKEDRQVFQAKSEIVEATPERLEALRGFQQDLEQLLTATA"
BASE COUNT          549 a          763 c          788 g          536 t
ORIGIN      
        1 ctgccagttc agagacagaa ggaatgccac ggtgttcagc gccagcagca cataagtcac
       61 gtacggagtg ccaacccggg gcgcgtcgtc tttcagcggg atcataggaa gtgggtcgct
      121 cgcaccgcat ggcagggaac cgaattccct tcaaattcct tgcggcagcc gatttctcgc
      181 cggcgatttg accaaaccac cgttgaacct acacgatagc agaaggatgc cgctcaatgg
      241 tgtgatcgtg gtggataaac ctgcgggatg gacttcgcac gatgtggtgg cgaaagcacg
      301 gcgcattttg ggcgaacgct ccattggcca cctgggaacc cttgacccca tggcgaccgg
      361 agtgctcccg ctggtcgttg ggcggatgac acggctctgc cagttctatt cgtcgtcgga
      421 aaaggcttac cagggaacaa tccggctggg tgtcgccacc gacacctacg attgtgatgg
      481 cgacccggtc ggaccagcgc aggatgttaa tgtaacactt gaagaaattc gggctgtggt
      541 cttcggcttc gtcgggttga tccagcagac tccaccctcg ttttccgcca agaagatcaa
      601 gggggttccg gcatacaagc tagctcgcaa gaagcaggaa gtcgagcttc agccggtcga
      661 ggtggaagtg aaggaattta cggtcctggg cctgaacggc gacctggtcg atttccggtg
      721 ccgggtttcg tcgggcacct atgtccgcag cctggcccac gaactggggc aaaagctggg
      781 catcggggcc cacctcgcga gcctccaccg gacggcggtg gccgagttcg ccatcgaaga
      841 gagccataca ttggacgaaa taggcgaggc tgccagccaa ggccgtattg aggagctgct
      901 agtccatccg cggcgtgtgc tgccggagat tccgtcggtc acggccagcg acgaggacat
      961 cgccaagatt cgccacggcc ggaccgtgaa cctgccggaa atgtcccgtt cgcgcttcgt
     1021 aaaggtcttc gccggacagt cggagttgat ctgcatcgcc agccgagtcg ccgggacgct
     1081 gttccatccc aaggtggtgc tgtgcagcta ggctgcgggt gtacactttg tcgtactttg
     1141 gagggatgac gctgtgcccg cggatgaaac tccggtattg cagttggaga tcgagaggcg
     1201 acccgaggcg aacatcgtgc actgcacggg caaggtggtg attgactcct gggccatgtt
     1261 caccgtggcg gtccgcgcac tcattcctga gggcaaggcg atccttgtgg acctggccaa
     1321 cgtgagcctg gtggatagtg tcggcatcgg caccttcgtc agcgtgtggg cgtcggcgcg
     1381 aaagggcggc tgcgagctga aattcatcaa tctgaacgaa aagatccacg atctgttcga
     1441 gatcacccgc ttgcacggcc tgtttggagg cgacgtcgcg ggcgcctaaa ccgctggaag
     1501 ggtcttcgct ttacaaaatc ctgagctgat cgttgaagtc gagggaagcc gcactggacg
     1561 gggcttgcgc tcacagtttg caccccatcc tcgcgtgatg tccgataaca gatattatgt
     1621 taagtggcga caaaatgatt cagaacccac ctcggtcctg cttggagaat cgcgactgtc
     1681 gtcgattgac ttagtccaca ccttagtcta ctgttgcccc atggaagtca atatccacga
     1741 agccaagacg catctctcca agctattgga gcgggttgcg tcgggtgagg aagtcattat
     1801 cgccaaggcg ggaaagccag tggcacggct tgtaccgatc aagcccaagc gtgctctacg
     1861 accactaggg ctggcccggg gcgaattcgt tgttcccgac gacttcgatg ctcccatgcc
     1921 ggatttcgaa gaggacttct atcgttgaaa gtcctcctcg acacgcacat atttctatgg
     1981 gcgatctcgg acccggaccg cctgtcttcg cggacgcaac agatcgtaaa ggatgcgcga
     2041 aacgacgtgt tgcttagtgt agtcagcgca tgggagattg ccgtcaagtc gagtctggga
     2101 aaactcgatc tgccgcgtcc cgccatggca tttgtccaac ggcagctcgc ccgtcaccga
     2161 attggcctcc tgccgcttag tctctctcac ctctccgcgc tagagagcct tccgtggcac
     2221 catcgcgatc catttgaccg aatgctggta gcacagtgcc gagaggaagg tgcaagtcta
     2281 atcaccgtcg ataaacaact ccatcggtat gaggtcgaga ttctctcctg aggggcgaca
     2341 cagcaccgcg tcaagcggtc gcagtcaata actgctccaa gtcctgctgg aacccccgca
     2401 gtgcctccag cctctccggc gtggcctcca cgatctcgga cttagcctgg aaaacctggc
     2461 ggtcctcctt ctccacgagc acgcccagca cttcccccat tcgccatccg ggcgttccga
     2521 tcatctgaag gccaaccggc gtggcccgca gcagcgcgat gcagccatcg cgaacgacgc
     2581 caatggaatt gggatagcgt tcaaaggttt gtagctcaaa tccggcgaga taaatg
//
LOCUS       DASRKK010000585        20284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1224354, whole genome shotgun sequence.
ACCESSION   DASRKK010000585 DASRKK010000000
VERSION     DASRKK010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224354"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..2685)
                     /locus_tag="VFC15_13830"
     CDS             complement(382..2685)
                     /locus_tag="VFC15_13830"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZM11281.1"
                     /translation="MSITKQDALEYHQGSRPGKIEVVSTKPCLTQRDLSLAYTPGVAD
                     PCLEIEKNPQDAYKYTAKGNLVAVVTNGTAVLGLGDIGALAGKPVMEGKGVLFKRFAD
                     IDVFDIELNTKDPDEIIRTCQLLEPTFGGINLEDIKAPECFYIEETLKKTMKIPVFHD
                     DQHGTAIISGAAIVNAVELVGKRMQDIKVVVNGAGASGIACAEHYVSLGVKRENITIC
                     DTKGVVYKGRTEGMNPYKERFAHDTPLRTLAEAVKGADVLLGLSKKGAFTPAMIASLG
                     PRPVVFAMANPDPEITYEEAKAVRDDLIMATGRSDFPNQVNNVLGFPFIFRGALDVHA
                     TTINEEMKLAATYALAALAKEDVPDSVCRAYGVVGRFKFGPEYIIPKPFDPRVLVWEA
                     TAVAKAAMDSGVAQNPVNLEDYRDQLEKRLGKAHELMRIMVHKAKANPKRVVFPEGSH
                     DRILRACHVLLEEQIAIPVLLGHGGEVEKAAAELGISLKGAVIVEPETASRRAEYVQE
                     FYRLRQRHGVTQASADELMNHRNIFGSMMVRMGDADALVSGVSQNYPNTIRPALQVIG
                     VREGIHKVSGIYLIITRKGDLYFLADATVNIDPSAEDLAEIALCAAQEARRFDVEPRV
                     AMLSFSSFGSTKHEYSDKMRRAAELARYADPTLIIDGEIMADIAVNAKKMEATFPFSP
                     LQGGANVLVFPDLQSCNIACKLLTNLGGAEAFGPILAGMAKPVHLLQRGAEVEDVVNV
                     TTIAVVDAQDSSVPYKIHVADPAMAVR"
     gene            complement(2954..4201)
                     /locus_tag="VFC15_13835"
     CDS             complement(2954..4201)
                     /locus_tag="VFC15_13835"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZM11282.1"
                     /translation="MGRLTGVLGILAILLFAYLFSTNRKAIRGRTILVGLTLQFIFAL
                     LILRVGYGERVMTAAGNAINRLLSYSFVGSEFLFGGLGSKYSKYGVIFAFQVLPTIIF
                     IAAFFAILYHFGVMQFIIKQFAKVMMRFMGASGVESMDVAASIFMGQTEAPLTIRPFL
                     PKVTQSELMVIMTAGMAHVSGGIMGAYILSGVEARHLLAAVIMTAPGTIVIAKMLVPE
                     TEKPLTAGTTKLDGLELEARDSNVLDAAAKGTTDGLHLALNVAAMLISFLALIALLDG
                     VLGGVHNLLAAHGFQWFPGSLETIFGVIFAPIAWLIGVPWHDCHSIGNLLGTRMVLNE
                     LVAFTMLGAEKASLDPRSVTIATFALCGFANFSSIGIQIGGLSALAPNRRHDLAKLGF
                     RAMIAGTLANLMSASIVGILLRS"
     gene            complement(4299..5024)
                     /gene="deoC"
                     /locus_tag="VFC15_13840"
     CDS             complement(4299..5024)
                     /gene="deoC"
                     /locus_tag="VFC15_13840"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZM11283.1"
                     /translation="MSVNSSVITHDPATLLQDWHSVASLIDHTLLSPSATRSQVIALC
                     AEAVRYGFYSVMVNPAYVTLAAAQVHGSPVKVGTVIGFPLGANLTVTKLAEAESAAQN
                     GAHDLDMVINIGALKSGGRVLVQTEMRSLADLAHAHGAILKVILETALLSEEEKILAC
                     ALAVEAGVDFVKTSTGFAASGATAADVALMRGVVGHEIGVKAAGGIRTAANLAEMVDA
                     GANRIGASASVAIVREFGAPSDE"
     gene            complement(5039..5572)
                     /gene="hpt"
                     /locus_tag="VFC15_13845"
     CDS             complement(5039..5572)
                     /gene="hpt"
                     /locus_tag="VFC15_13845"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZM11284.1"
                     /translation="MDKLKVLLSREQIASKVAELGERITHDFEGESIVLIGVLKGATI
                     FLADLARQISLDVSFDFIAVSSYGISKQQSGEVKLNKDVDHSMEGKNIILVEDILDTG
                     LTLTYLKNLLLGHQPKAVKIAALLDKVSRRTQPISGDYVGFEIPDEFIVGYGLDFAER
                     YRNLPDLCILETDDQSG"
     gene            5719..7347
                     /locus_tag="VFC15_13850"
     CDS             5719..7347
                     /locus_tag="VFC15_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11285.1"
                     /translation="MLRRSQRTAILVGILVLTGLVTAGAFAANRPGVSSEARTEHYFE
                     NIRKDPNLLTAFLLEMPKGGDLHNHLSGAIYAETMIGWAKDERQCVDPKTLYLTPSLK
                     LSTGDPYCPAPTVLAETAFANPVLYRSMVDAFSMRNWELSGQSGHDHFFDTFDKFGAA
                     THGNNGKMLAETANRAVSQREVYQELMFTPTGKPFGDMLNSDAVKAIKLTDNTTPETF
                     AAMRKALTENGLSSALQDAIQQTNEAEKIRDTEMGCGLAGATPGCKLTQRYLFQVLRG
                     LPKEIVYAQMLLGFELAMADLRFVGLNMVMPEDYYVPMHDFPLHMRMMQYLHSQYPNV
                     HVTLHAGELVEGLVPPEGLRFHVRDSVEMAGAERIGHGVDTLNETNALELLNELAKKN
                     VMVEICLTSNDVILGVRGKDHPLHDFMRAGVPVALATDDEGVSRSDMTHEYLKGVEDQ
                     NLSYSDLKRMARTSLQHAFIQGSSLWNDGKTFAPVKECASVQSVTTPSATCQKFVDGS
                     QKARLQWQLEQQFREFEGREWPAVTAESSRAAVR"
     gene            complement(7313..7723)
                     /locus_tag="VFC15_13855"
     CDS             complement(7313..7723)
                     /locus_tag="VFC15_13855"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZM11286.1"
                     /translation="MKKKLTPETEKLLWDAAEGAAALAYAPYSTFHVGAALLTSEGEI
                     VTGCNVENASYGLTNCAERTAIFRAIAEQKLSRGMAIEAIAVVHRDHQSCTPCGACRQ
                     VIYEFGHNAIVLYEYEGKRKQTAISALLPESFQL"
     gene            complement(7728..8759)
                     /locus_tag="VFC15_13860"
     CDS             complement(7728..8759)
                     /locus_tag="VFC15_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11287.1"
                     /translation="MPVFRSRLIPCVLAILLLANSAWPWGNEGHMMINRVAAEKLPPD
                     VPAFLRNAAEQLAYLGPEPDRWREKSELALKLSQEPDHFLDMELVEGMTLPPDRYSFY
                     RALEAKRQQTPGQSENLTPEKVGLQPYVTMEVFGRLIVAFREYRHALRDHTNPEFSER
                     DVIFYAGWLGHYVADGANPLHTTVKYNGWTGANPNGYTTEHTIHWKMEGNFVAANQKD
                     LKFADLVPATASKLDSPFQDYLAYLHKSNGYVEQVYQIEKAGGFEGAGSPESQQFIEQ
                     RMAFGAEMLRDLWYTAWLDSAVDPPPYVPPKPASSTAPASKPAPSDSPAPNMRTAPKL
                     GEPTPQPGG"
     gene            complement(8762..9379)
                     /locus_tag="VFC15_13865"
     CDS             complement(8762..9379)
                     /locus_tag="VFC15_13865"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZM11288.1"
                     /translation="MNWAKGNLGWIEVVCGPMFSGKSEELIRRLRRAEIARQRVQIFK
                     PGIDNRYSSDHIVSHSDLKIRSTAVRDSADIEAQLDLRTEVIGIDEAQFLGMELEDLV
                     VRLADMGKRIIIAGLDTDYLGRPFHPIPELLAVADEITKTLAICMQCGNPAKHTQRLV
                     ASEDLIVVGTSGMYEARCRRCFEPNLAKLEKLEPAVRAKASETAK"
     gene            complement(9376..9888)
                     /locus_tag="VFC15_13870"
     CDS             complement(9376..9888)
                     /locus_tag="VFC15_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11289.1"
                     /translation="MLAYLSGSIEYSPDHGKAWRAALTPFLRSLGHDVYDPAEDEKKN
                     LTEVEGREFRGWKRSDLPRFQQTIRKIIAYDLDWIEHRSDYIVAYWDEYASRGAGSQA
                     ELSVAHRRGIPVYLVTELPVGDVSGWILGCASEVFSSFDQFKQFMMAKYRITDTKAHV
                     SPVAQSAGMP"
     gene            complement(10008..10970)
                     /gene="egtD"
                     /locus_tag="VFC15_13875"
     CDS             complement(10008..10970)
                     /gene="egtD"
                     /locus_tag="VFC15_13875"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZM11290.1"
                     /translation="MATSKQLQFQANAATASAVREGLGAAPKWLPAKLFYDEAGSALF
                     EQITELPEYYLTRTERSIFESFATEILQSAGPSLTLVELGAGTAAKTCVLIEELLRRQ
                     PRALFYPIDVSTSALQEAATQLGRQFPQLRVNPIIADYTGGVEALNRISGRKLVLYIG
                     SSIGNFEPDESVRVLRRIRRSLRTGDALLLGADFAKSSKILIPAYDDPQGVTAAFNKN
                     ILARINRELEANFDLDAFRHVALWNRRCSRMEMHLESAVAQQVFVPMLDMDINFSQGE
                     RIHTENSYKYTPELIETILRESGFKLEQSWHDRQKWFGVHLARV"
     gene            complement(10960..12255)
                     /gene="egtB"
                     /locus_tag="VFC15_13880"
     CDS             complement(10960..12255)
                     /gene="egtB"
                     /locus_tag="VFC15_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZM11291.1"
                     /translation="MASASTIRGSADPVITAGDLNLTQQYVSVRQTSLRCCAPLTVED
                     HSLQSMPDASPAKWHLAHTTWFFETFLLTEHLPDYPVFRPAFRNLFNSYYNAVGDRPP
                     RALRHTLSRPALEEVRAYRSHVDEAMTRLLSRELAPEVLDLVTLGLNHEQQHQELILT
                     DVKNGLALNPLRPAYREVGNNGTNSPGSATELRWRSFPEGIYTVGFEGSDFAFDNESP
                     RHNVYLAPFRLASRPVTNGEYIQFMLDNGYSTAALWLSDGWDCVRANQWSAPLYWEMR
                     DGRWWHYTMDGMRPVGEAEPACHVSFYEADAFARWAGERLPTEFEWEVASRSCAIEGN
                     FVDSGAFHPQPASESGALTQMFGDVWEWTASAYLPYPGFQAAAGAVGEYNGKFMCNQM
                     VLRGGSCATPRSHIRRSYRNFFPPHARWQFMGIRLANGD"
     gene            complement(12419..13213)
                     /locus_tag="VFC15_13885"
     CDS             complement(12419..13213)
                     /locus_tag="VFC15_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZM11292.1"
                     /translation="MSEPTHSFILPAYNESERLTTSIPKVLEYVHARNLRAEIIVVND
                     GSTDKTAEVVRQFAAANPMIILLENPGNRGKGYSVRHGMLHAKGAVALFTDADLSSPI
                     TEADKLFAALADGADVAIGSRWLQRDLQTERQPFYRQLYGRLFNLGLSIVLRLDYRDT
                     QCGFKAFTRQAIQVVFTRQRVERWGFDPELLFLARKYKLKTVEVPVEWAHDHRSKINP
                     LRDGLRMGLDVLQIRWNSLMGRYKRASKALSDAAPAQPSAPVRLTK"
     gene            13697..14038
                     /locus_tag="VFC15_13890"
     CDS             13697..14038
                     /locus_tag="VFC15_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11293.1"
                     /translation="MDRVTGRITQIKLPDFDPYYSDVSWYRDYAAYCGVADHGEKVSV
                     IVTQISTRKPLYKKQIEKLGGEFPGSICDSPTWERQPARVTFTPHGGEKQTVEVNGRF
                     ADLTPDTPAEE"
     gene            14056..14637
                     /locus_tag="VFC15_13895"
     CDS             14056..14637
                     /locus_tag="VFC15_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZM11294.1"
                     /translation="MRTFDELLRDAEIAHGHLCAGQVLGVRMAMLGLELLGIDDPRGK
                     DRKRLVTYVEIDRCMTDAVAVVTGCRLGKRALKFRDWGKVAATFVDLESGRAVRIAAR
                     ESSKALARQTHPEIDGKNQQQMLAYREMPTDDLFAVQWVKVTVPPQELPGYKGERMVC
                     AECGEGINFQREVRKDGKILCHACAGERYYETL"
     gene            complement(14701..14997)
                     /locus_tag="VFC15_13900"
     CDS             complement(14701..14997)
                     /locus_tag="VFC15_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZM11295.1"
                     /translation="MHSLTVLYPNKEGAKFDFEYYVDKHIPFANGLLGHEFKVTKGIA
                     SAQGGPPAFLCTARMEIGTTEEFLPVLIQHVAALQNDIPNYTNIVPVIQFEELL"
     gene            complement(15016..16470)
                     /gene="gatA"
                     /locus_tag="VFC15_13905"
     CDS             complement(15016..16470)
                     /gene="gatA"
                     /locus_tag="VFC15_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZM11296.1"
                     /translation="MKIELLTVDSARTAVLEKQITATALVDAFYQKIEAEDPAIGAYL
                     TLCKDRAHRQAEWVDAMADKGDALPPLAGVPIAIKDVLTTKGVRTTAGSRILDNFIAP
                     YDATAVARLEAAGAIVLGKTNCDEFAMGSSNENSGFHPVKNPRDHSRVPGGSSGGSAA
                     VIAAGTAVASLGSDTGGSIRQPGAFCGVVGLKPTYGRVSRYGLIAFASSLDHVGPFGK
                     TVKDVALILQQMAGHDPLDSTSANLPVPNYVQQIGEPVKGLKIGVPKEYFGEGLDPEV
                     RAAVEAGIQELAKSGCEIEPVSLPHTEYAIPTYYLIATAEASSNLARFDGVRYGVRSK
                     EAKTLAEMYRKTRDAGFGTEVKRRIMLGTYALSAGYYDAYYLKAQRVRSLLARDFDEV
                     FTKVDAIVTPTTPTPAFKLGEKADDPLAMYLADIYTVTANLVGVPGITVPCGNSKEGL
                     PIGLQILGKHFDEATVLKTAHVVEHAMAASATSA"
     gene            complement(16489..16815)
                     /gene="gatC"
                     /locus_tag="VFC15_13910"
     CDS             complement(16489..16815)
                     /gene="gatC"
                     /locus_tag="VFC15_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZM11297.1"
                     /translation="MVSEKDVTYVADLAHLELTVDERARMLKDLNSILGYIERLNQLD
                     TANVEPMAQVASRYGVSKDSTGSEAFAYAMRPDELRPSLPHDEAMSNAPHTDGTFFKV
                     PKVIER"
     gene            complement(16834..17133)
                     /locus_tag="VFC15_13915"
     CDS             complement(16834..17133)
                     /locus_tag="VFC15_13915"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZM11298.1"
                     /translation="MQHARGTLKKIFTQNVCAKAGDEAPLLAWPVACGGAVAANTQAL
                     SFVDGVLVVAVPDAVWRNQLQFMSQQYLAGLNQIAGQMVRSISFVVAPHAIRPTT"
     gene            complement(17308..18660)
                     /locus_tag="VFC15_13920"
     CDS             complement(17308..18660)
                     /locus_tag="VFC15_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZM11299.1"
                     /translation="MKLHAIHVVRELRKHGFEAYFAGGCVRDMLLGLEPTDYDVTTNA
                     VPNEVMRIFPETYAVGAQFGVVLVPVPKDNDHETVPPEHPHAIEVATFRSDGNYSDGR
                     HPDQVQYSKSPEEDVQRRDFTINGLLMDPLDGDRVLDFVGGRADLAAGIIRAIGDPRR
                     RLQEDKLRMLRAVRFAARFGYAIDPPTFAAIQKLAPTIHQVSRERVRDELTKMLTEGH
                     ARKAFELLDSTWLLKEVLPEVDRMHGVEQPPQYHPEGDVWIHTLMLLEQLQPDCSRTL
                     AWGALLHDVGKPPTFRVAPDRIRFDGHVEVGVRMAHEICQRLHFSNDDTAQIEELVAN
                     HMRFADVERMKASTLKRFLRLPMFEEHMALHRIDCLSSHQDLSLYNFVSEKVRTTPPE
                     QIRPHALISGADLIALGYRPGPLFKEVLSAVEDAQLEGKLHSREEALELVRNEFPLAR
                     "
     gene            18811..19956
                     /gene="tsaD"
                     /locus_tag="VFC15_13925"
     CDS             18811..19956
                     /gene="tsaD"
                     /locus_tag="VFC15_13925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZM11300.1"
                     /translation="MSDTYILGIESSCDETAAAVVRSGEELISNVVYSQIATHQPYGG
                     VVPELASREHLRAIVPVVRKAIEDAGKTYDTIDAIAVTQGPGLAGALLVGLSYAKALA
                     YAADKPLIAVNHLEGHIHAVLLEERQGGRHDLEFPVLALVVSGGHTHLYLAERNETSW
                     TYRNIGHTRDDAAGEAFDKVAKLLELGYPGGPSIDVLARNGNPHAVNFPFAQIKHRDR
                     NPKNRHTLDERRTDFSYSGIKTAVLRYVEIHEMAADIKHRREIISAIATPTPQDYLAA
                     CDRQTLDLIASFQRAIVDDLVSKTLAAAIDHGVATLLVTGGVAANSELRTTFEARATE
                     EGIAVLFPSRKLSTDNAAMIAAAAYPRFLAKEFADSEVSSEPALLLG"
     gene            complement(20028..>20284)
                     /locus_tag="VFC15_13930"
     CDS             complement(20028..>20284)
                     /locus_tag="VFC15_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11301.1"
                     /translation="TIPDNSQLSGSFGQHLMNFIQPSIARQSHPQSEAMDQKCLHPRV
                     QIVSRDEDAEFVECIECREIFESSEFRDMDIEAKIPTEEE"
BASE COUNT         4278 a         5930 c         5849 g         4227 t
ORIGIN      
        1 ccttactcac agactaaagt gatggctaga ttcaccactt ttatggctaa agcacaatta
       61 aattaacggg tcaggagggg gagaagcggg aaaaagtgag ggcggaactc tgtccggtag
      121 cgctgcgccc aggtgcgcta ccgaagggac catgaagatc cgagaattcc gccgactgac
      181 gaagtgtgaa actcaggacg aacgtaaacg tctggcccaa ctacctgctg ctgaactgca
      241 aacatcccat tgctcgtgat tgtgccatcg tctgccgggc agacctatcc ctcttccatc
      301 gtagtggctt acaacttagg ttgtagtcga gccgctgact tccagcggcc cgactacact
      361 tcgccgatca accactttca gctaccgcac cgccatcgca ggatcggcaa catgtatctt
      421 gtatggcact gagctatcct gcgcatcgac cacggcgatg gtggtgacat tgaccacatc
      481 ctcgacttca gctccccgtt gcagcaggtg caccggtttc gccatacctg ccaggatcgg
      541 accgaaggct tccgccccac ccaaattggt cagcaacttg caagcgatgt tgcaggactg
      601 cagatccggg aacaccagca cattggctcc gccctggagc ggcgagaatg gaaacgttgc
      661 ttccatcttc ttggcgttga cggcgatatc ggccatgatt tcgccgtcaa tgatcagcgt
      721 cggatcggca tagcgcgcca attcggcggc ccgccgcatc ttgtcggagt actcatgctt
      781 ggtgcttcca aagctggaga acgacagcat cgcgacccgc ggctccacgt cgaagcggcg
      841 tgcctcctgc gcggcgcaaa gtgcgatctc agccaggtct tctgcgctgg gatcgatgtt
      901 caccgtggca tcggccagga agtagagatc gcccttccgg gtgatgatca ggtatatgcc
      961 ggaaaccttg tgaatgcctt cccgcacacc gatcacctgc agcgccggac ggatggtgtt
     1021 gggatagttc tgcgaaacgc ccgagaccag cgcatcggca tcgcccatcc ttaccatcat
     1081 ggaaccgaag atgttgcggt gattcatcag ctcgtccgca gacgcttgcg ttacaccgtg
     1141 gcgttgccgc agccggtaga attcctggac gtattcggca cggcgagagg ccgtctcggg
     1201 ctcgacaatc actgcacctt tgagcgagat acccaactcc gcggccgcct tctctacttc
     1261 gcctccgtgg ccgagtaaca ctggaatggc gatctgctcc tccaaaagga catggcaggc
     1321 acgcagaatt ctgtcgtgcg agccttccgg gaacactacg cgcttgggat tggccttggc
     1381 cttgtggacc atgatgcgca tgagctcatg ggccttaccc aaccgcttct ccagttggtc
     1441 gcgatagtct tccaggttaa ccgggttttg cgcgactccg gagtccattg ccgccttggc
     1501 gacagcggtt gcttcccaca ccagcacgcg cggatcgaac ggcttcggga tgatgtactc
     1561 aggtccaaat ttgaaccgcc ccaccacacc gtaagcgcgg cacactgagt caggcacatc
     1621 ttccttggcc agcgcggcca gggcataggt tgccgccagc ttcatctctt cgttgatggt
     1681 agtggcatgc acgtccagag cgccacggaa gatgaacggg aacccgagaa cattgttgac
     1741 ctggttgggg aagtcggagc gtcccgtggc catgatgagg tcgtcgcgga ccgctttggc
     1801 ttcttcatag gtaatttccg gatcgggatt cgccatggcg aagaccaccg gtcgcggacc
     1861 aagtgaggcg atcatcgcag gggtgaaagc acccttcttg gagagcccca gcaggacatc
     1921 cgctcccttg actgcttccg ccagcgtgcg cagcggagtg tcgtgagcga agcgttcctt
     1981 atagggattc atgccttccg tgcggccctt gtaaaccacg cccttggtat cgcagatggt
     2041 gatgttttcg cgtttcaccc ccaacgatac gtagtgctcg gcacaggcga tgccggaagc
     2101 tcccgcacca ttgactacga ccttgatgtc ttgcatgcgt ttgccaacca actccacggc
     2161 attcacgatc gcggcaccgg aaataatcgc ggtaccgtgc tggtcatcgt ggaagaccgg
     2221 aatcttcatc gtcttcttca gggtctcttc gatgtagaag cactcggggg ccttgatgtc
     2281 ctcgagattg atgccgccaa aagtaggttc gagaagctgg caggtgcgga tgatttcatc
     2341 gggatctttg gtgttcagct cgatgtcgaa cacgtcgatg tcggcgaacc gtttgaacag
     2401 cactcccttg ccttccatga cgggcttgcc ggccagtgcg ccgatgtcgc cgaggccgag
     2461 cactgccgtg ccattcgtta ccaccgcaac caggttgccc ttggcggtgt atttgtaggc
     2521 gtcctgaggg ttcttttcga tttcgagaca gggatcggca acgcctgggg tgtaagccag
     2581 gctcagatcg cgctgcgtca ggcaaggctt ggtcgatacg acttcaatct ttcccggacg
     2641 ggatccctgg tggtattcaa gtgcgtcctg cttagtaatg gacatgatgg tctccgtatt
     2701 gaacgatgag gaattaggtg cggagaaacg aagcagacgc gggaacggcc gtacagctta
     2761 ccaccgagca acgggagact catgaacggc gaacggacgt cttgttgcac ttcagcgcac
     2821 gcaggcttaa gagcctccag ccggctctta acctctccag catggcctga acgggaactc
     2881 ggaaatagtg cacaggtcac aaccagttgt gaggcctgac gcacttcctc ggcggccgat
     2941 ctcggattcg tccctacgat cgcagcagga ttcccacaat gctggccgac atcaaattcg
     3001 ccagagtacc ggcgatcatg gcgcggaatc ccagcttcgc cagatcgtga cgccggttcg
     3061 gcgccaacgc actaagtccg ccaatctgaa tcccgatcga gctaaagttg gcgaatccgc
     3121 acagtgcaaa cgtcgcgatg gtgaccgacc gcgggtcaag cgatgccttc tctgcgccca
     3181 gcatggtaaa tgccaccagt tcgttcagca ccatgcgggt tcccagcagg tttccaatgg
     3241 aatggcagtc gtgccaggga actccgatga gccaggcgat gggtgcaaaa attaccccga
     3301 aaatggtttc aaggctgccg ggaaaccact ggaaaccgtg agcggcgagc aggttgtgca
     3361 ctcccccgag aacgccgtcg agcaatgcga taagggcaag aaacgaaatc agcattgccg
     3421 ccacgttcag cgcgaggtgg aggccgtccg tggtgccctt ggcagcagcg tcgaggacgt
     3481 tgctgtccct ggcttcgagc tccaggccat cgagcttggt agtgccggcg gtgagaggct
     3541 tctcggtttc cggaaccagc atcttggcaa tcacaatggt tcccggcgcc gtcatgatga
     3601 cggcagcaag cagatgacga gcttcgacgc cgctaaggat gtacgccccc atgattccgc
     3661 cggagacgtg cgccatgccg gccgtcatga tgaccatcaa ttccgactga gtgaccttgg
     3721 gtaagaacgg acgaatcgtg agcggagctt cggtctggcc catgaagatg ctggcggcaa
     3781 cgtccatcga ctcgacgccg ctggcgccca tgaagcgcat catcaccttg gcaaattgct
     3841 tgatgatgaa ttgcatcacc ccgaagtgat aaagaatggc aaaaaacgca gcgatgaaga
     3901 tgatggtcgg cagcacctgg aaggcgaaga tcacgccgta tttagaatat ttgctgccca
     3961 gtccgccaaa cagaaactcc gagccgacga atgagtagct cagcagacgg ttgatggcat
     4021 tccccgcggc ggtcatcacg cgctcgccat accccacacg caggatcagc aacgcgaaaa
     4081 tgaattgcag cgtaagtccg accaggatgg tcctgccgcg aatagctttg cggttggtcg
     4141 aaaacaggta ggcgaacagc agaatggcga ggataccgag cacgccggtc agacgcccca
     4201 tgccaactcc tgcgcgcgat tgtaagggca gctatgaagt ggtgcgataa acttcgtcag
     4261 acgtgactct gagagattgc ggtgattgat tgtcatggct attcgtctga tggagcgcca
     4321 aactcgcgca cgattgcgac actggcgctg gccccgattc ggttcgcgcc ggcatcgacc
     4381 atctcggcaa gatttgccgc tgtacgaatt ccacccgctg ccttcacccc gatttcgtgc
     4441 cctacgactc cgcgcatgag agcgacatca gcagcagtcg cgccggaggc agcgaatccg
     4501 gtggaagtct tcacgaaatc aactcccgcc tccaccgcca gagcgcaggc gagaatcttc
     4561 tcctcctcgc tcagaagcgc ggtttcgagg atcactttca ggatcgcgcc gtgggcatgg
     4621 gccaagtccg ccaggctgcg catctcggtc tgcaccagca cgcggccccc agacttgagc
     4681 gcgccgatat tgatcaccat gtcgagatcg tgagcgccat tttgcgcggc tgattcagcc
     4741 tcggcaagct tggtaaccgt gagattcgca cccaatggaa atccgatcac ggtgccgacc
     4801 ttgaccggcg atccgtgaac ctgggcggcc gccagcgtca catacgccgg attgaccatg
     4861 accgaataaa atccgtagcg caccgcctcg gcacaaaggg caatcacctg gctgcgcgtg
     4921 gctgatgggc tgagcaatgt gtgatcgatg aggctggcca cggaatgcca gtcttgcaag
     4981 agggtcgctg gatcgtgagt gatgacagaa gagttgacag acataggcac gtgcgattct
     5041 accccgactg gtcgtcagtt tccagaatgc aaagatccgg caggttacga tagcgctcgg
     5101 caaagtctaa tccgtagcca actatgaact cgtcgggaat ctcgaaaccg acgtaatcgc
     5161 cgcttatcgg ctgggtgcgg cgcgagactt tgtcgagcag ggccgcgatc tttaccgcct
     5221 taggctgatg cccgagcaac aggttcttca ggtaggtcag cgtcaggcca gtgtcgagaa
     5281 tgtcttccac caggatgatg ttcttgccct ccatggagtg atcgacgtcc ttattcagct
     5341 tcacttcccc gctctgttgc ttagagatgc cgtagctgga aacggcgatg aaatcgaagc
     5401 tgacgtcgag gctgatctgg cgggcaagat cagctagaaa aatcgtcgcg cccttcagca
     5461 ctccgatcag tacgatcgac tcgccctcga aatcgtgggt gatacgttcg ccaagttcgg
     5521 caactttgct ggcgatttgc tcgcggctga gtaggacttt gagtttgtcc aagggcatcc
     5581 ccacggtgtg attcttaaga aacttggatg gtagagaatt ggcggacgaa actcaactaa
     5641 acaatcatgg gcgcgcggct gttatgatgc cgcattctgg aaaccctagc tgttcccctg
     5701 acctgggagg ccttatctat gcttcgtcgt tcgcaacgca cagcaattct cgtcggcatt
     5761 ctcgttctga caggcctggt gacagcaggc gcgtttgcag ccaatcggcc gggcgtttcg
     5821 tccgaggccc ggacggaaca ctacttcgaa aatatccgga aagacccgaa tctgcttacc
     5881 gcgttcctgc tcgaaatgcc caagggaggg gatctgcaca atcacctgtc aggcgcaatt
     5941 tatgcggaga cgatgatcgg gtgggcgaag gacgaaaggc agtgcgtcga tcccaagaca
     6001 ctctatctca ctccatcgct gaagttgtcg acgggcgatc cgtattgtcc ggccccaacc
     6061 gttctcgccg aaaccgcgtt cgcgaacccc gtcctgtatc gcagcatggt cgatgctttc
     6121 tccatgcgca actgggaatt gtccggacaa tccggacacg accacttctt cgataccttc
     6181 gacaagtttg gtgcggccac ccacggaaat aatggaaaga tgctggcgga gaccgcgaat
     6241 cgcgcggttt cgcaacgcga ggtctatcag gaactgatgt tcacgcccac gggaaagccg
     6301 ttcggcgaca tgctgaactc agatgcggtg aaggcaatca agctgacgga caacaccaca
     6361 cctgagactt tcgccgccat gcgcaaggcc ctgaccgaga atggcctgtc gagcgcatta
     6421 caagatgcga tccaacagac caacgaagcc gagaagattc gcgacaccga aatgggttgc
     6481 ggtttggcgg gcgccactcc gggctgcaag ttgacgcagc gctatctatt ccaggtgctg
     6541 cgtggcctgc ccaaagaaat cgtgtacgcc cagatgttgc tgggctttga actcgccatg
     6601 gccgatctgc gatttgtcgg actgaacatg gtcatgcccg aggattatta cgtccccatg
     6661 cacgacttcc cgctccacat gcgcatgatg cagtatttgc acagccaata tcccaatgtg
     6721 cacgtgacgt tgcatgcggg cgagttggtc gaaggactgg ttcctcctga aggcctgcgt
     6781 tttcacgtgc gggattcagt ggaaatggcc ggggccgagc gcattgggca tggagtggat
     6841 accctcaacg aaacgaatgc ccttgaattg ctcaacgagt tggcaaagaa aaacgtgatg
     6901 gtcgaaattt gtctgaccag caacgacgtc atcctagggg tgcggggcaa agatcatccc
     6961 ctgcacgatt tcatgcgggc aggtgtgccg gtcgctttag ccacggatga cgaaggtgtt
     7021 tcgcgctccg acatgacaca tgaatatcta aagggtgtcg aggatcagaa cttgtcgtac
     7081 agcgacttga agcgcatggc gcgaaccagc ctgcaacacg ccttcatcca gggctccagt
     7141 ctgtggaacg acggcaagac gtttgcgccg gtgaaggagt gcgcgagcgt gcaatccgtc
     7201 acaacgccaa gtgcaacttg ccagaaattt gtggacggca gtcaaaaggc gcgattgcaa
     7261 tggcaattgg aacagcagtt ccgcgaattt gagggccgtg aatggccagc ggttacagct
     7321 gaaagctctc gggcagcagt gcgctgatcg cagtctgttt gcgtttgcct tcgtactcgt
     7381 aaagcacaat ggcattgtgg ccaaactcat agatgacctg acggcacgct ccgcacggag
     7441 tgcagctctg gtggtcgcga tggaccaccg cgatcgcttc gatcgccatc ccgcgtgaca
     7501 gcttttgttc ggcaatggcc cgaaatatcg cggtccgctc cgcgcaattg gttaatccgt
     7561 aggaagcgtt ttcgacgttg cagccagtta caatctcgcc ctcgctggtc agcaaggcgg
     7621 cgcctacgtg gaaggttgaa taaggtgcgt aggccagtgc agccgcgccc tcggccgcgt
     7681 cccacaacag cttttcggtt tccggagtga gcttcttctt cattacatta accgcctggc
     7741 tggggggttg gttcacccag cttgggagct gtccgcatat tgggagcagg tgagtcggat
     7801 ggcgctggct tggacgccgg agcggtggaa ctggctggct tcggaggaac atacggcggc
     7861 ggatcgaccg cgctgtcgag ccacgcggta taccaaagat cgcgtaacat ctccgcgccg
     7921 aaagccatgc gctgctcgat gaattgctga gactccggac tgccggctcc ctcaaaacca
     7981 ccggccttct cgatctggta aacctgttca acgtagccgt tcgatttgtg gaggtaggcg
     8041 agatagtcct gaaaagggct gtcgagtttg ctcgccgtgg ccggcaccag gtcggcgaac
     8101 ttcaggtctt tctgattggc agcaacgaag ttgccttcca tcttccagtg gatggtgtgt
     8161 tccgtggtgt agccgttggg attggcgccc gtccagccgt tgtatttcac tgtggtgtgc
     8221 agtggatttg cgccgtcggc aacatagtgt cccagccaac cggcgtagaa aatgacatcg
     8281 cgctcggaaa attccggatt cgtgtggtcg cgcaatgcgt gccgatattc gcgaaaagcg
     8341 acaatcagcc ggccaaacac ttccatcgtg acgtagggtt gcagaccgac cttttcgggc
     8401 gtcaggttct cggactgccc gggcgtctgc tgccgtttcg cctccaacgc gcgataaaaa
     8461 ctgtagcggt ccggaggcaa cgtcatgcct tcgaccaact ccatgtccag aaagtggtcg
     8521 ggctcttgcg acagcttcag cgccagttcc gacttctcgc gccagcggtc cggctcagga
     8581 ccgagatagg ccaactgctc ggccgcattc cgtagaaatg caggaacatc gggcggcagc
     8641 ttttccgccg ccacccgatt gatcatcatg tggccttcgt tgccccaggg ccaggcagaa
     8701 tttgccagca gcaagatcgc gaggacacac gggatcaggc gggatcggaa aacaggcatt
     8761 gctatttcgc ggtttccgaa gccttcgcac gcacggcggg ttcgagcttc tccagttttg
     8821 ccagattagg ctcgaagcag cgccgacagc gggcttcata catgcctgag gtccccacca
     8881 cgatcaggtc ttcgctggcg accaggcgtt gggtatgctt ggctggattg ccgcactgca
     8941 tgcagatagc cagcgtcttg gtgatctcgt cagccaccgc cagcaattcg ggaatgggat
     9001 ggaaaggccg ccccaggtag tcggtgtcga gcccggcaat gatgatgcgc tttcccatgt
     9061 ccgccagccg gacgacaagg tcctcaagtt ccatgcccag gaactgggct tcatcaatgc
     9121 caataacttc agtgcggaga tcgagttgcg cttcaatgtc agcggagtcg cgcactgccg
     9181 tcgagcggat cttgaggtcg ctgtgcgaaa caatgtggtc ggaggagtag cggttatcga
     9241 ttccgggttt gaagatctgc acccgctggc gcgcgatttc ggcccggcgc agacggcgaa
     9301 tcaactcctc gctcttgccg ctgaacatgg ggccgcaaac cacttcgatc cagccgagat
     9361 ttcccttggc ccagttcatg gcatacccgc cgactgcgcc accggggaca cgtgggcttt
     9421 ggtatcggta atacgatact tcgccatcat gaactgctta aactggtcga agctggagaa
     9481 gacttccgag gcgcaaccca gtatccagcc gctgacgtcg cccaccggca actcagttac
     9541 caggtaaacc ggaatgccgc gacggtgtgc cacggacaat tccgcctgcg agccggctcc
     9601 gcgcgaggca tattcgtccc agtaggcgac gatgtagtcc gaacgatgtt cgatccagtc
     9661 gagatcgtaa gcgatgatct tgcggatggt ttgctgaaag cgcggcaggt cggagcgttt
     9721 ccagccgcgg aattcgcgcc cttcgacctc ggtgaggttc ttcttctcat cctcggcggg
     9781 gtcatacaca tcatgcccga gcgatcgtag gaacggggtc agcgcggccc gccaagcctt
     9841 gccatgatcg ggggagtatt cgatggaacc tgaaagataa gccagcatcg tggaaaagtg
     9901 attgtcgtct gtcgcgccgc aaaggtcaat tgtcggtggc gataaggctg aggaaatcca
     9961 gcgattgcat ccgagatggt gcagtacagc gacgaaatcg ctttctatta cacccgcgcc
    10021 aggtgcactc caaaccattt ctggcggtcg tgccagcttt gctccagttt gaacccgctc
    10081 tcgcggagaa tcgtttcgat cagttccggc gtgtacttgt agctgttctc ggtgtggatg
    10141 cgctcgcctt gcgagaagtt aatgtccatg tcgagcatcg gaacaaacac ctgttgcgcc
    10201 accgcgcttt ccaagtgcat ctccatgcgc gagcagcggc gattccacag ggcgacgtga
    10261 cggaaggcat cgagatcgaa gttcgcttcc agctcgcgat taatccgcgc caaaatgttc
    10321 ttgttgaaag ctgcggtgac tccttgcggg tcgtcgtacg ctgggatcaa tatcttggaa
    10381 ctcttggcga aatcggcgcc tagtaacaaa gcatctcccg ttcgcaagct gcgacgtatg
    10441 cggcgcagca cgcggacaga ctcatcgggc tcgaagttgc cgatactgga accgatgtag
    10501 agcaccagct tgcgtcctga aattcggttc agcgcctcga cgcccccggt gtagtcggcg
    10561 atgatcggat tgacgcgcag ctgcggaaat tgccgtccta attgcgttgc cgcttcctgc
    10621 aacgcactgg tggaaacgtc gatcgggtaa aacagcgccc gcggttggcg gcgcaacagc
    10681 tcttcgatca ggacgcaggt tttggctgcc gttccggcgc ctagttcgac cagggtcagc
    10741 gacggccccg cgctctgtaa gatttccgtc gcgaagctct caaaaatcga gcgttccgtg
    10801 cgcgtcaggt agtactcggg cagctccgtg atctgttcga aaagcgcaga accggcctca
    10861 tcgtaaaaca acttcgccgg cagccacttg ggggctgcgc cgagcccttc gcgcaccgcc
    10921 gaggcggtag ccgcattggc ctggaactgc agttgtttgc tagtcgccat tggccaaccg
    10981 aatgcccatg aactgccagc gcgcatgggg tggaaagaag ttgcgatagc tacgccggat
    11041 gtgggaccgc ggggtcgcgc aagaccctcc gcgcagcacc atctggttgc acatgaactt
    11101 gccgttgtac tcgccgaccg cgccagccgc tgcctgaaat ccgggatacg ggagataagc
    11161 gctcgcggtc cactcccaga catccccgaa catctgggtc aatgctccgc tctcgctcgc
    11221 gggctgagga tggaaggctc cgctgtccac aaagtttcct tcgatggcgc acgagcgcga
    11281 cgcgacttcc cattcgaact cagtaggcag acgttcgccc gcccatcgag cgaaggcatc
    11341 ggcttcgtag aaactaacgt gacacgctgg ctcagcttca ccaaccgggc gcattccgtc
    11401 catcgtgtag tgccaccatc ggccgtcccg catctcccaa tacagcggag cgctccactg
    11461 attggcgcgc acgcagtccc agccatccga cagccacaac gcagcggtgg agtacccgtt
    11521 gtcgagcatg aactgtatgt attcgccgtt ggtcaccggc cgcgaagcca gccggaaggg
    11581 cgcaaggtac acattgtggc gtgggctctc attgtcgaag gcgaaatcgc tgccctcgaa
    11641 tcccacggta tagatacctt cgggaaaaga tcgccaccga agctcggtag cagacccagg
    11701 ggagttggtg ccgttattac cgacctcgcg ataggcgggt cgcagcgggt tcagcgccag
    11761 cccattcttg acgtctgtca gaatcagctc ctggtgctgc tgctcatggt tcagacccaa
    11821 tgtgacaagg tcgagtacct ctggagccag ttcgcgcgac agcaggcggg tcatcgcttc
    11881 atccacatgg ctgcgatacg cgcgcacctc ttccagcgcc ggtcgcgaga gcgtgtgccg
    11941 cagcgcgcgc ggcgggcggt cgccaaccgc gttgtagtag gaattgaaaa gatttcggaa
    12001 ggcgggacgg aacacggggt agtccggcaa gtgttcggtc agaagaaatg tctcaaagaa
    12061 ccatgttgtg tgcgccagat gccacttcgc gggactggca tcgggcatgg attgcaggct
    12121 gtgatcttca accgtcaatg gcgcgcaaca tcgcaaagag gtttggcgga cgctgacgta
    12181 ctgttgagtc agattcagat cgcctgccgt tataactgga tctgccgacc ctcggattgt
    12241 ggaagcactc gccatgatgt cgttcgaaaa tgttgccgat taagttcaaa acgcttccag
    12301 tcaagatgcc agactgcgcc ggaagctgca aattttaatt ggaaattcga ttgttacgaa
    12361 ctacggcaaa gatcggattg acagtgcacc tcggcgcatt ccgaacgcgg ataacacttc
    12421 acttcgtcag ccgcacgggc gctgacggct gagccggagc cgcgtccgaa agcgctttag
    12481 acgcccgttt atagcggccc atcagcgagt tccagcgaat ctgcagcacg tccaagccca
    12541 ttctcagccc atcacgcaaa ggattgattt tcgagcgatg atcatgcgcc cactccaccg
    12601 gaacctcaac cgtcttcaac ttgtactttc tggcaaggaa cagcaactcg ggatcgaagc
    12661 cccacctttc tacccgctgg cgggtaaaga ctacctggat cgcctggcgg gtaaacgcct
    12721 tgaacccgca ctgagtgtcg cggtagtcaa gccggagaac gatgctcagt cccagattaa
    12781 aaagtcgccc atatagttgg cgatagaacg gctggcgctc cgtctgcagg tcgcgctgga
    12841 gccagcgcga tccgatggca acgtccgcac cgtcggccag tgccgcgaac aatttatctg
    12901 cctcggtgat gggtgacgaa agatcggcgt cggtaaacag agcgaccgcg cccttggcgt
    12961 gcaacattcc atggcgcacg ctgtatccct tgccgcgatt gcccgggttc tcgagcaaaa
    13021 taatcattgg gttggcggca gcaaactgac gcacgacttc cgccgtctta tcagtcgagc
    13081 catcgttcac gacgatgatt tccgcccgca gattgcgagc gtgaacatac tccagcacct
    13141 tcgggatcga ggtggtgagt cgctcgctct cgttgtaggc cggaagaata aagctatggg
    13201 tcggctcgct catgggggat tcgacgtttg ctcattgtat gacagttgtc agcaggcaaa
    13261 cgtagctgag ggaatcctgt acctgtcgtg cccaccagtt ggatgcgtag ggccgcccag
    13321 aatgcactgg gtactttcat aacttgcagc ccgcggggga cggggctacg cttgcctcga
    13381 tgctatcccc cgaaattatc tccgttgtcc tgctgctcgt gatctccgcg ggcgcggctt
    13441 ccaagcccca cgtaatgagc ctgggaaaac ccatgccggt caaattattc ctaagctgag
    13501 cggaagacaa gaccgtcacc atcaatgtcc gccctcttta tttggacacc aagctcaagg
    13561 aatacaccgc cggggcaacg cacgatgtga ccgaccgtct gttcgtggtg cggcgggcgt
    13621 accgcgtcaa cgattcgctt cctggcgagc ggcaagtcag cctaagtggc tttggcagcg
    13681 cggcggctgg ctgctggtgg atcgcgttac cggccggatt acgcagatca agctgcccga
    13741 tttcgatccc tactactctg acgtaagctg gtatcgcgac tatgcagctt attgcggcgt
    13801 ggccgaccat ggcgagaaag tctccgtcat cgtgacgcaa atcagtactc gcaaaccgct
    13861 gtacaagaaa cagatcgaga aactgggagg cgagtttccc ggatcgattt gcgattcccc
    13921 gacttgggaa cgccaacccg cgcgcgtcac gttcactccc catggaggcg aaaaacagac
    13981 cgtagaagta aatggccggt tcgccgattt gacgcctgat actcccgccg aagaatgacg
    14041 taaactgttc gcaccgtgcg gaccttcgac gaacttttac gcgatgccga aatagcccac
    14101 ggacacctgt gcgccgggca agtccttggt gtgcgcatgg ccatgctggg actcgaactg
    14161 cttggcatcg acgaccctcg cggaaaagat cgcaagcgtt tggttaccta tgtagaaatt
    14221 gatcgctgca tgaccgacgc cgtcgccgtc gtcaccggat gccgcctcgg caagcgagcc
    14281 ctcaagtttc gggactgggg aaaagtcgct gctacgtttg tcgatctgga atcgggcagg
    14341 gcggtgcgca ttgccgctag ggagagttcg aaggcactag ctcgccagac gcaccccgaa
    14401 atcgacggca agaaccagca gcagatgctg gcctatcgcg agatgccgac cgacgatctg
    14461 tttgccgtac agtgggtgaa ggtcacggtg ccgccccagg aacttcccgg ctataaaggc
    14521 gagcggatgg tgtgcgccga gtgcggcgag gggatcaact tccagcgcga agttcggaag
    14581 gatggaaaga ttctgtgcca cgcctgcgcc ggggaacgct attacgagac gttgtagggc
    14641 gaagttgtcg ggcgcggaca ccacccgaag aatcgcgcgc ccctcgtcgg cgcactcttg
    14701 ctacagcagt tcttcgaact gaatgacagg aacaatgttt gtgtaattgg gaatatcgtt
    14761 ctgcaacgcc gcgacatgtt ggatgagaac tggcagaaac tcctccgtcg taccaatctc
    14821 catccgtgcg gtgcagagga acgcaggcgg gccgccctga gcagacgcta tgcccttcgt
    14881 aactttgaac tcatgcccca gcagtccatt cgcaaatggg atgtgtttgt ccacgtagta
    14941 ctcaaaatcg aacttggcgc cttctttgtt tgggtacagt acggtcaagc tgtgcatatt
    15001 ttgatcttcc ctctttcaag cggatgtcgc tgaagccgcc atcgcatgtt ccaccacgtg
    15061 tgccgtcttc agcaccgtcg cttcgtcgaa atgcttgccc agtatctgca acccgatcgg
    15121 caatccttct ttgctgttgc cgcacggcac ggtgattccc ggaacgccaa ccaggttggc
    15181 cgtgaccgtg tagatgtccg ctaggtacat ggccagcgga tcgtctgcct tttcgccaag
    15241 tttgaacgca ggtgttggag ttgtcggagt gacaatggca tccaccttag tgaacacctc
    15301 gtcgaagtcc cgggccagca ggctgcgcac ccgctgggcc ttcagataat aggcgtcata
    15361 gtatccggcg cttagagcgt acgtcccaag catgatgcgg cgcttcactt ccgtcccaaa
    15421 tccggcgtcc cgcgtcttgc gatacatctc ggccagagtc ttcgcttcct tggatcggac
    15481 tccgtagcgc acgccgtcga agcgcgccag attcgacgat gcctcggcgg tcgcgatcaa
    15541 gtagtaagtt ggaatggcgt actcagtatg cggcagagat actggctcta tttcgcaacc
    15601 cgacttagcc agttcctgga ttccggcctc caccgcggcc cggacctctg gatctagacc
    15661 ctcgccgaag tattccttgg gtacgccgat cttaagtccc tttaccggct cgccaatctg
    15721 ctgcacatag ttcggtacgg gcaagttggc cgaggtcgaa tccaagggat cgtggcccgc
    15781 catctgctgc aggatcagcg caacatcctt caccgtcttg ccaaaggggc cgacgtgatc
    15841 gagcgacgaa gcaaacgcaa tcaagccata acgtgagaca cgtccgtagg tcggcttcaa
    15901 tccgacaacg ccacagaacg ctccgggctg gcgaatcgag ccgccagtat cggaacccag
    15961 cgacgctact gcagttccgg cggcaatcac ggcggccgaa ccgcccgacg aacctccggg
    16021 aacgcgcgaa tgatcccgcg gattcttcac tggatgaaac ccggaattct cattcgacga
    16081 gcccatggca aactcatcgc agttggtctt gcccagcacg atcgcgcctg cggcttcgag
    16141 tcgggcgacg gcagtggcat cgtaaggcgc gatgaaattg tcgagaatcc tcgatcccgc
    16201 ggtggtgcga acgcccttgg tggtcagcac atccttgatc gcaatcggca ctccggccaa
    16261 cggaggcagc gcgtcgccct tatcggccat cgcgtcaacc cactcggcct ggcgatgagc
    16321 gcggtccttg caaagcgtga ggtaagcgcc gatggccggg tcttccgctt caatcttctg
    16381 gtagaacgca tcgaccagtg ccgttgcggt gatctgcttc tccagaaccg ccgtccgcgc
    16441 cgaatctacg gtcagtaatt caatcttcat cgcgtgtccg ttcggctcct agcgctcgat
    16501 gactttcggc actttgaaga atgtgccgtc ggtgtggggt gcgttgctca tggcttcgtc
    16561 gtgcggcagc gaaggacgca attcatcggg ccgcatggca taggcaaacg cctcgctgcc
    16621 tgtcgagtcc ttgcttactc catatcggct ggccacctgc gccatgggct cgacgttggc
    16681 ggtatcaagc tggttgagcc gctcgatata acccagaatg gagttcaggt ccttcaacat
    16741 gcgagcgcgc tcgtcgacgg ttagctcgag atgcgcgaga tcggcgacgt aagtgacgtc
    16801 tttttccgaa accatgaagt gtcgtcctcg taattatgtt gtgggacgga ttgcgtgcgg
    16861 cgctaccaca aacgaaatgc tccgcaccat ttgccccgcg atttgattca gtcccgcaag
    16921 atattgctga ctcatgaact gcaactgatt gcgccacact gcatcgggca cggccaccac
    16981 cagcactccg tcgacaaagc tcagggcctg ggtattcgcc gcgaccgcgc caccgcaggc
    17041 caccggccac gccagcaaag gagcttcatc gccagccttg gcgcaaacat tttgcgtaaa
    17101 gattttcttg agtgtcccgc gtgcgtgctg catctctgaa ggttgatttt agcatcgctc
    17161 tgtcgaaaat cattcaccac tgagacatgg agggcacgga atcttattaa caagatctct
    17221 gcgactccgt ggtaattttc agcttcttgg gtgggccgaa gaactatgga cactccgggg
    17281 tgatacatcc ccacccttta aacagagtta ccgggccagg ggaaactcat tccgcactaa
    17341 ctctaacgcc tcttccctgc tgtgcaattt gccttcgagt tgcgcatctt cgaccgctga
    17401 caatacctcc ttgaagagcg gtcccggccg atagccgagc gcaatcaggt cggcacctga
    17461 aatgagcgcg tgcggcctga tctgctcagg aggagtggtc cgcactttct cgcttacaaa
    17521 gttgtaaagc gacaaatcct ggtgactgct gaggcagtcg atgcgatgca gcgccatgtg
    17581 ctcctcgaac atcggcagcc gcaggaagcg cttcagggtc gaagccttca tgcgctcaac
    17641 gtcggcgaag cgcatatggt tggccaccaa ctcctcgatt tgcgcggtgt cgtcgttaga
    17701 gaaatgcagg cgctggcaaa tctcgtgcgc catacgcact ccgacttcca catgtccatc
    17761 gaagcggatg cggtcaggcg cgacgcgaaa tgttggaggc ttgccgacgt cgtgcaacag
    17821 cgctccccaa gccagtgttc gcgaacaatc tggctgcaat tgctccagca gcatcagggt
    17881 gtggatccaa acgtcgccct cgggatgata ctgcggcggc tgctccacgc catgcatgcg
    17941 atcgacctcg ggcagtactt ccttcaacag ccacgtgctg tcgagtaatt caaaggcttt
    18001 tcgcgcatgg ccttccgtca gcatcttggt caactcatcc cgcacccgct cccgactcac
    18061 ttgatgaatc gtaggagcga gcttctgaat cgcggcgaac gttggtggat cgatcgcgta
    18121 tccaaagcgc gcagcaaacc gcaccgcacg cagcatccgg agtttgtctt cctgcagacg
    18181 acgccgggga tcgccaattg cgcggatgat tcccgcagcc agatccgcgc ggccgccgac
    18241 gaagtcgagc acgcggtcac cgtcgagcgg atccataagc agcccgttaa tcgtgaagtc
    18301 gcggcgctgc acatcttctt ccggcgactt ggaatactgc acctggtcgg gatgacgtcc
    18361 atcggagtag ttgccgtcac tgcggaaggt ggccacttcg atggcgtgag gatgctcggg
    18421 cggaacggtt tcgtggtcgt tgtctttcgg aaccggcacc agcaccacgc caaactgcgc
    18481 acctaccgcg taggtctcgg gaaagatccg catgacttcg tttggaaccg cgttggtggt
    18541 gacgtcgtaa tccgttggtt caaggcccag aagcatgtca cgcacgcagc ccccggcgaa
    18601 gtaggcctca aagccgtgct ttcgaagctc gcgaaccacg tgaatagcgt gcagtttcat
    18661 gtttgaagag attgtattcg ttcggagaac ggccaatggc tgtccagata gaaggtaact
    18721 ggtaactggc aactgggaag tacaaataat ctggaagtgg ggtaggcatc gccagtcacc
    18781 agttaacagt cacccacgta caatgaaatc gtgtccgaca cctacatcct cggcatcgag
    18841 tcctcctgcg acgagaccgc cgccgccgta gtccgctcgg gcgaagaact gatttccaac
    18901 gtggtttatt cgcagattgc gactcaccaa ccctacggtg gggttgtccc ggaactggcc
    18961 tcgcgcgagc atcttcgcgc cattgtcccg gttgttcgca aggcgatcga ggacgccggc
    19021 aagacctacg acaccatcga cgccatcgct gtcacacaag gccccggtct cgccggagcg
    19081 ctgctggttg ggctcagcta cgccaaggcg ctggcttacg cggccgataa gcccctgatc
    19141 gcggtgaatc acctcgaggg ccacatccat gccgtgctgc tcgaagaacg acaaggcggc
    19201 cggcacgatt tggagttccc cgtgctcgcc ctggtcgtca gcggtggcca cacacatttg
    19261 tatttggcag agcgaaacga aactagctgg acgtatcgca acatcgggca cactcgcgac
    19321 gatgctgcag gcgaagcgtt cgataaagtt gccaagttgc tggaactcgg ctatcccggc
    19381 ggcccgtcga tcgacgtgct cgcacggaac ggcaatcctc atgcggttaa ttttccgttt
    19441 gcacagatca agcatcgcga ccgcaatcca aaaaaccgcc atactttaga tgaacgccgc
    19501 accgacttct cctacagtgg catcaagacc gcggttctcc gttacgtgga aattcacgag
    19561 atggcagcgg atatcaaaca tcgacgcgag attatttcgg cgattgctac gccgactccg
    19621 caggattatc tggcagcctg tgatcgtcag actctggact tgattgcgtc atttcagcgc
    19681 gcaatcgtcg acgatctcgt cagcaagacg ctcgctgcag cgattgacca tggcgtagca
    19741 acgttgctgg taacgggagg tgtggccgcc aacagtgagt tgcgaactac gttcgaggca
    19801 cgcgctacag aagagggcat cgcggtactg tttccttcgc gcaaactctc caccgataac
    19861 gcagcgatga tcgcggccgc tgcgtatccg cgattcctcg ccaaggaatt cgccgactcg
    19921 gaagtgtcat ccgaacccgc tctccttctc gggtagctcg caatcttcag cgcgtagaca
    19981 cacttccgcg ctctcgccca agctcccggt tgttcgacaa aatctgctta ttcttcttcg
    20041 gtgggaatct tcgcttcgat gtccatgtcg cggaattcgc tcgattcgaa gatctcgcgg
    20101 cactcgatac attccacgaa ctccgcgtct tcgtcacggg aaacgatctg gacacgagga
    20161 tgaagacact tctgatccat ggcctcgctc tgggggtggc tctgccttgc aatcgacggc
    20221 tggataaaat tcataaggtg ttgcccgaac gagccggaca attgggaatt gtccggtatt
    20281 gtag
//
LOCUS       DASRKK010000586         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_736495, whole genome shotgun sequence.
ACCESSION   DASRKK010000586 DASRKK010000000
VERSION     DASRKK010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736495"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFC15_13935"
     CDS             <1..277
                     /locus_tag="VFC15_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11302.1"
                     /translation="QACVALEMDGSTVKSARVVLGHVAPIPWISPEAAQALVGKSISP
                     ETAMAAANAAVAQAKPLSHNKYKVTLAKAAVKRAILSAAGQSSGGAA"
     gene            274..513
                     /locus_tag="VFC15_13940"
     CDS             274..513
                     /locus_tag="VFC15_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11303.1"
                     /translation="MMLNLDADRFDTKREDLCPALRWKGQFIDVEHDPTVPPTNDGLF
                     WCMHTQTCIGPDGELAEPGTCSSMSRACHGTGKCG"
     gene            complement(972..1472)
                     /locus_tag="VFC15_13945"
     CDS             complement(972..1472)
                     /locus_tag="VFC15_13945"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM11304.1"
                     /translation="MTADEVKQLLQLQPLAIEGGFFRETYRSRWNVAAEYLPDGIRGP
                     RSIGTAIYYMITPETFSALHRLPGSEIFHFYLGDPAIMLQLLPDGSSRTITLGSDLAG
                     GQQPQVVVRGGVWQGCKLASGGKFALMGTTMSPGFDYADYETGVRDELIAQYPGAAEN
                     IREYTK"
     gene            complement(1550..1939)
                     /locus_tag="VFC15_13950"
     CDS             complement(1550..1939)
                     /locus_tag="VFC15_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11305.1"
                     /translation="MTSDIALANSSLPTQIDQELVLLETLKELLTVAMGSLSGGEAGN
                     LMEVGRQISSTADDLMALASHLGHLPLTPEEQQQRRKLLAELGQQRAFCRAMLRRWRR
                     SILLPRHLLALHGEAETYTEPMEARWS"
BASE COUNT          429 a          664 c          600 g          426 t
ORIGIN      
        1 gcaggcgtgc gttgcgctcg agatggacgg cagcacggta aagagcgcgc gcgtcgtgct
       61 cggacacgtt gcgcccatcc catggatctc gcccgaagcg gcgcaggcgc tggtggggaa
      121 gagcatctct cccgagaccg cgatggcggc cgcgaacgct gcggtcgcac aagccaaacc
      181 gctcagccac aacaagtaca aagtcacgct ggcgaaggcc gctgtaaagc gcgccatcct
      241 aagcgccgcg ggccagagct ccggaggtgc cgcatgatgc taaatctcga cgctgatcgc
      301 ttcgacacca aacgcgaaga cctttgtccg gcgttgcgct ggaagggaca attcatcgac
      361 gtagagcacg atcccacggt gccgccgacg aatgacggat tgttctggtg catgcacacc
      421 cagacctgta tcgggcccga tggtgaattg gccgagccgg gaacctgctc gtcgatgtct
      481 cgcgcgtgcc acggaaccgg aaagtgcggc taattccact tacccgcgaa agactggagg
      541 cgctcacatg aaaggatttc ttcgaggctg gtcctcgttg tagtcatcac cacgaacata
      601 gctgctcagt ctactgcgga accatcgcta acgtcggcag gattgccgac ctatcctcca
      661 ctagccgcgc aggggcgtat ccaagggaca gtgaaaatat ccttcgtgtt ggattcgaac
      721 gggaacccaa cagacgttcg agcaatttcg gggcacccaa tgcttaagcc ttttgcggtt
      781 gccagcgtta agacttggaa attcgaaccg ccgaaaaacc tgtcggaatc gcggcagtac
      841 gaaacggaat tcgtttaccg cctttccggg aacgaagttg atcggcttcc ttcattgact
      901 gtttctttaa aatcatttca gcatatagag attgtgacgg acgtcgtcag atcgaccgta
      961 agctattagt tttacttggt atactctctg atattctccg ccgcgcccgg atattgcgcg
     1021 atcaattcgt cgcggactcc tgtttcataa tccgcgtagt cgaacccggg cgacattgtc
     1081 gttcccatca gcgcgaactt gccgccggag gcaagcttgc atccctgcca tacacctcca
     1141 cgaaccacca cttgcggttg ctgtccgccg gcgagatcgc ttccgagcgt aatcgtccgc
     1201 gagcttccgt cgggcagcaa ctgcagcatg atcgcgggat cgccaagata gaagtgaaag
     1261 atctccgagc cgggcagccg atgcagcgcc gagaacgtct ccggcgtgat catgtagtag
     1321 attgccgtgc cgatggagcg cgggccacga atgccgtcgg gcaggtattc cgccgcgacg
     1381 ttccagcgtg agcgataggt ttcacgaaag aagccgccct cgattgccag cggctgcagt
     1441 tgcagcagtt gtttgacctc atccgcagtc atggtgggaa caggctatca ctccgcacaa
     1501 cgggaacgct ctatccccgg tagcgttgcc ggtatccagg ctgctcttat caagaccagc
     1561 gcgcttccat cggctcggtg taggtctccg cttcgccatg cagcgccagc aaatgccgcg
     1621 gcagaagaat cgaacggcgc catcgccgca gcatcgcccg gcagaatgcg cgctgctggc
     1681 ccagttccgc cagcagcttg cgccgctgct gttgctcctc gggggtcaag ggcagatgcc
     1741 ccagatggct cgccagcgcc atcaggtcgt ctgccgtcga actgatctgc cggcccactt
     1801 ccatcaggtt tcccgcctcg ccgccgctca aggatcccat tgcaaccgta agcaactctt
     1861 tcaaggtctc cagcagcacc agctcctgat cgatctgggt tggaagagaa gagttcgcca
     1921 atgcaatatc cgaagtcaac ttgcacctcc tcaggcgata tcgtctaact gatgactgcc
     1981 ctgctcctcc gccagatctt ctgcctccgc cagctcgctc tcatgcttgg accggcgcgg
     2041 tgcacgccct ttgcgctctc ccgctggcgt gctcaacggg cgctccgcgg tccgcgatgc
     2101 tgcttccagg cggccagtg
//
LOCUS       DASRKK010000587         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1126916, whole genome shotgun sequence.
ACCESSION   DASRKK010000587 DASRKK010000000
VERSION     DASRKK010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126916"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..863
                     /locus_tag="VFC15_13955"
     CDS             228..863
                     /locus_tag="VFC15_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZM11306.1"
                     /translation="MSDERAKKIKLMLFDVDGVMTDGTIFLFPAPGAGAGQDTHERRE
                     QMADQGGFGFAGQKMIEAKGFNAHDGAGFSLARLGGLKTGLITKRISETVALRARDLK
                     IEHVYQGQQDKASAFYEILAKEGLAPQEVCYVGDDVIDLPVMRLCGLAIAVKNARPEV
                     LRESHYVTPHEGGKGAVRDAIEYVLREQGSLDRVIDEYIRSRAADQAPTAE"
     gene            1888..3144
                     /locus_tag="VFC15_13960"
     CDS             1888..3144
                     /locus_tag="VFC15_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZM11307.1"
                     /translation="MLKRFAIVVMNTLLALFVCANAPAATISSHKTHTKTHHITETPA
                     AQKKMAGMRTSSKTVASRNRSTHVVSSTHVVVSKKHRYSERFSASSFADDQVEGDITA
                     GEDPIVRAAALEALGNMNGTVVAVDPDNGRILAMVNQKLALSAGAQPCSTIKVAVALA
                     ALSENVITKDTQVPLGKYWRVDLTTALAHSNNAYFEAVGRKLGFERVSYYAHQFGLGE
                     LAGWHIDGEHLGTFPSTELDQKLGGVGKMCSFGESISLTPLQLAGLISSIANGGTLYY
                     LQHPTTPEQVTSFTPKVKRKLDIGPLIPEISDGMAGAVEYGTARQLRANFIEEPVLGK
                     TGTCSKDGTRFGWFGSFANTQYGRIVTVIFLEGGRPTFGPKAAELAGHLYRNLYDHEF
                     FAVKPPTTPVRTAGSETNPTAVGAPQ"
BASE COUNT          756 a          969 c         1014 g          661 t
ORIGIN      
        1 gaccttcaaa agcggcgcca gagtcatgag caccccaacc gcgcaggccg attcaggcga
       61 ttctgcgggg aacccgcatt actcattcgg cgagaatcgc gcttgctaga agctaagagc
      121 taaaggctaa tggccaagag ccaagagcga aaagcgaaag gcgaagagcg aagaacgaac
      181 agcgaacagc gaacagcgaa gagcggcacc cctgctacac tggccgcatg tccgacgaac
      241 gcgcgaagaa gatcaagctg atgctgttcg acgtggacgg cgtgatgacc gacggcacga
      301 tttttttgtt cccggcgccg ggcgcgggag caggtcagga cacccacgag cgtcgcgagc
      361 agatggccga tcagggcggc ttcgggttcg ccggccagaa gatgatcgag gccaaaggat
      421 tcaacgcgca cgatggtgca ggattttcgc tggcccggct gggcgggctg aagacaggcc
      481 ttatcaccaa gcgaatttcc gaaaccgtgg cgctgcgggc gcgagacctt aaaatcgagc
      541 acgtctatca agggcagcag gacaaggcgt ccgcctttta cgagattctg gcgaaggaag
      601 gcctggcgcc gcaagaggtc tgctacgttg gggacgatgt catcgatctg ccggtgatgc
      661 ggctgtgcgg gctggctatt gcggtcaaga acgcccgacc ggaagtgctc cgcgagtcgc
      721 actacgtaac gcctcatgaa ggcggcaaag gcgccgtccg cgatgcaatt gagtacgttt
      781 tgcgcgaaca gggttcgctt gatcgcgtta tagacgagta catccgatcc agagccgccg
      841 atcaggcacc cacggcggaa taaagatccg agtcagaagc acgaggaaat cggggcagaa
      901 aaatcccctc cgaaggacag gggcgaattg cacttcggag ggatagccgc ttttacacga
      961 ccattctcag ggttgttatc gaatcaaaca caccgccttt ggggaagttt cgctcaccgc
     1021 gaaaaagcta tcggaattcg acaaacggct ggtggagaag gcattcggga ggctgcagag
     1081 cgtgcgagaa ctgtgccgtc cctaaaggga ctcgatctta gttttccact ttacccagcg
     1141 cttccgcgct gggctaacta agcgctttcg ccccatttgg ggctggttct gtatgctccc
     1201 ttccactgca gcacaccccg acaaggtcgc gacacagcct cttgtgccct ggctaaaacc
     1261 aattccgtcc ctcctccacc ccattttcgc ccgccgcggc gggcgcgaac tggggacccc
     1321 ggctgcaggg tcagattttg cgcaaacgtt gtaccgagcc gactcaaaac gtgttctccc
     1381 acatctttct cggtcctgtc ccgagtgtca tcgggcctgc ggcccaccga aattatccac
     1441 ggagatcacg gagaaactct atggaaatca aaactccgtg ccctctgtgt tctccgtggt
     1501 gaacgatttt cgacggagcg acccgcccag cctcctccaa agcggaacgg caatctgact
     1561 ctccaaaatc gcacaattct gccggggaat cagccttgct acggttgcag agtggggaaa
     1621 gtgtgataag taatcaattg ggaactggta gcgggtcgcc ggttgtcata agtggcaagt
     1681 cgtcgagttt cgcaactcgg gtctaagccc aagcatctgt tttgcttggg cgcatcggtt
     1741 ttgccgttgc aacaacagtt ttaagttgaa tccggaaccc aaaataccta ctatgggaaa
     1801 tctcgcaacc gaagacggtc tatcgtgggg agcaagaatt tttccggcga ggaacgttac
     1861 agagtccggg gaagccaagg gttgtcaatg ctaaagcgtt tcgccatcgt cgtgatgaat
     1921 actttgttgg cgctatttgt gtgcgccaac gccccggctg ccaccatctc cagccacaag
     1981 actcacacca agacgcatca catcaccgag acccccgcgg cgcaaaagaa aatggccggt
     2041 atgcgcacca gcagcaagac ggtggcgagc cgcaatcgtt ccacgcatgt cgtctcttcc
     2101 actcatgtcg tcgtctcgaa gaagcatcgt tattccgagc gcttctccgc cagctccttt
     2161 gccgacgatc aagtggaagg cgacatcact gcgggtgaag atccaatagt ccgcgcggcc
     2221 gcgctggaag cgctgggcaa tatgaacggc acggttgtcg cggttgaccc cgacaatggc
     2281 cgcatcctgg cgatggtgaa tcagaagctg gcgttgtcgg ctggagcgca gccgtgttcg
     2341 acgatcaaag tcgcggtggc gttggcggcg ctcagcgaga acgtcataac caaagacacg
     2401 caagttccgc tgggcaagta ctggcgcgtg gacctgacca cggcgctggc gcactccaac
     2461 aacgcctact ttgaggccgt gggacgcaag ctgggcttcg agagggtgag ctattatgcc
     2521 caccagttcg gactcggcga acttgcgggc tggcacatcg acggtgagca cttgggcacg
     2581 ttcccctcga ccgagcttga tcagaagctg ggcggcgtcg gcaagatgtg ttcgtttggc
     2641 gagagcattt cccttacccc gctgcaactg gcagggctga taagctcaat cgccaacggc
     2701 ggcaccctgt attacctgca gcatcccacc accccggagc aagtgacaag cttcacgccg
     2761 aaggtcaagc gcaagctcga catcggtccg ctgattcccg agatttccga cggcatggcc
     2821 ggggcagtgg agtacggcac ggcgcgtcag ctacgcgcga acttcatcga ggagccggtg
     2881 ctgggaaaga cgggcacttg ttccaaggat ggcactcgct tcgggtggtt tggttccttt
     2941 gccaacacgc aatatgggcg gattgtgacc gtcattttcc tggaaggtgg ccggccgact
     3001 tttggtccaa aggcggcgga gttggcgggg cacctgtacc gcaacctgta tgaccacgaa
     3061 ttcttcgcgg tgaaaccgcc aaccacgcca gttcggacgg cgggttccga gacgaatccc
     3121 acggccgttg gcgctccgca atagccaaac cggacttacg aacagcaaag gcctcccaaa
     3181 gcgcggaggc ctttgcaaca tctgtcaact gctgcagtga cagctacttc gcgaagggcg
     3241 agcccgactg ggcttgcagc agacgcagtg cttcctgata gatatttttc gcggatgcgt
     3301 cttgggtggt gctgattttg tcattgagcc acttcaacat ggccgctcgc gagggatgag
     3361 ggaaatttgg atcggcgtcg ttaatgcgcc attcgtcgtt
//
LOCUS       DASRKK010000588         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1225086, whole genome shotgun sequence.
ACCESSION   DASRKK010000588 DASRKK010000000
VERSION     DASRKK010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225086"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFC15_13965"
     CDS             <1..904
                     /locus_tag="VFC15_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZM11308.1"
                     /translation="PCNDNSADYHVAATASCDSRCTIDAGPGAMAIPIKMDDRLRRIH
                     SGQNARVKELRRTFGEAAPNAEGDVAVEGMHVVEEAIRSGLRLTTVFFSESAKERVHK
                     LLPQLSSHTEALLLPDEVFASAVPSETPQGVAALVRVKSFALDEVFAPAPALLVAIAG
                     LQDPGNLGTIARSAEAFGVTGLLLGERTVSPWNWKAVRASAGSLFRLPALKVELAKAL
                     REIKARGVSVLATSSHKGTAIAEVDLRAPVTFIVGSEGAGIPKDVLALADEVVAIPQS
                     SRVESLNAGVAASILLYEAARQRR"
     gene            987..1406
                     /locus_tag="VFC15_13970"
     CDS             987..1406
                     /locus_tag="VFC15_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HZM11309.1"
                     /translation="MDYCVEHESEVLEQGHLLHSELTGQIIAAAVEVHRELGPGLLES
                     AYQACMCRELSLRRIDFRTEVALPVDYKGIHLDCGYRMDLVVAGTIAVELKSIDKIVP
                     IHQAQLLTYLRLSGMRVGLLVNFNVSILRHGIIRRIL"
     gene            1454..>2648
                     /locus_tag="VFC15_13975"
     CDS             1454..>2648
                     /locus_tag="VFC15_13975"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZM11310.1"
                     /translation="MFQNNSAPDDAIDKSRPLADRMRPRNLDEFVGQEHILAPGKPLR
                     VQIERDDTGSLLFWGPPGVGKTTLAKIIARMTHAEFVEFSAVLTGIKEIKQVMADAER
                     ARQYGTRTIVFVDEVHRFNKAQQDAFLPYVEKGSIRLIGATTENPSFEVISALLSRCR
                     VYVLQPLTEEQIVLLLQRALEDTERGLGEAHLEAEDDVLRKIAAYSSGDARSAYNVLE
                     VAATLAAEQQKQGERPRLTEGIVTDALQKRVLRYDKAGEEHYNLISAMHKSVRNSDPD
                     AALYWLARMIEAGEDLLYIARRVVRMASEDIGLADPNALAITLAARDAVDFLGVPESN
                     LALAQAVIYLAVAPKSNALYTAYGAVQEDVERTAQEPVPLHLRNAPTGLMKALDYGKG
                     YQYAHD"
BASE COUNT          528 a          765 c          848 g          507 t
ORIGIN      
        1 cccttgcaac gacaactcag cagattacca cgtcgcggcc acggcaagct gtgatagccg
       61 gtgtacgata gacgccggtc ccggcgccat ggccattccc atcaaaatgg acgatcgttt
      121 acgccgcatc cacagcgggc agaatgcgcg agtgaaggaa ctgcgccgca ctttcggcga
      181 ggctgcgccc aatgccgagg gcgacgttgc cgtggaaggc atgcacgttg tcgaagaggc
      241 gatccgcagc ggattgcggc tcactaccgt cttcttcagc gaatcggcga aagaacgcgt
      301 gcacaagctg ctgccgcagt tgtcgtcgca caccgaggcg ctgctgctgc cggatgaggt
      361 ttttgccagt gccgtgccca gcgagacgcc gcaaggcgtg gctgctctgg tgcgcgtgaa
      421 atcgtttgcc ttggacgaag tgtttgctcc ggcgccggcg ttgctggtgg ccatcgccgg
      481 gctgcaggat cccggcaatc ttggcacgat tgcgcgctcc gccgaagcgt ttggggtcac
      541 cggactgctg cttggcgagc gcaccgtcag cccctggaat tggaaggcgg tgcgggcttc
      601 cgcaggatcg ttgttccggt tgccggcgct gaaggtcgaa ttggcaaaag cgctgcgcga
      661 gatcaaagcg cgcggcgtaa gcgtgctggc gacgtcctcg cataaaggga cggcgattgc
      721 ggaagtggat ctgcgcgcgc cggtgacctt catcgtcggc agcgaaggtg cgggcatccc
      781 caaggacgtg ctcgcgctag ccgacgaggt ggtggcgatt ccgcagtctt ctcgcgtcga
      841 gtcgctcaat gcaggcgttg cggcgtcgat cctgttgtat gaggccgcgc ggcaaagacg
      901 gtgaaccctt tcacgacgga ggtcacggag agcacggagg aagaaagaat agaatcgttt
      961 tgttcgcttc agaacggagg tgtttcgtgg actattgcgt ggagcacgag tcggaagttc
     1021 ttgagcaagg ccatctcctt cattcggagt taacaggaca gatcatcgct gcagcagtcg
     1081 aagtgcatcg cgagttgggt cccggtttgc tcgaatccgc atatcaagcg tgtatgtgcc
     1141 gtgaactttc gctgcggcgc atagatttcc gaactgaagt cgcactgccc gttgactata
     1201 agggtatcca cctggattgc ggttacagaa tggatttggt ggtcgccggg acgatcgctg
     1261 tcgagcttaa atccatcgac aagatagttc cgattcatca ggcccaactt ctgacgtatc
     1321 tgcggttgag cggcatgcga gtagggctct tggttaactt caacgtctcg atattgcggc
     1381 acggaatcat tcgaagaatc ctttgattcc tccgtgctct ccgtgtcctc cgtggtgaac
     1441 ccaaagagtg agcttgttcc agaacaattc ggcgccagat gacgcgatcg acaaatcacg
     1501 gccgctggcc gatcgcatgc gtccgcgcaa cctggatgag ttcgtcggac aggaacacat
     1561 tctcgcgccc ggcaagcccc tgcgggtcca gatcgagcgc gacgacaccg gctcgctgct
     1621 tttttggggc cctcctgggg tcggaaaaac caccctcgcc aagatcatcg cgcgcatgac
     1681 ccatgccgag ttcgtcgaat tttccgctgt gctgaccggc ataaaagaaa tcaagcaggt
     1741 gatggccgac gcggaacgcg cgcgtcagta cggcacgcgg accatcgtct tcgtggacga
     1801 agtacaccgc ttcaacaagg cgcagcagga cgcctttctc ccgtacgtcg aaaagggcag
     1861 catccgcctg ataggcgcca ccaccgaaaa cccgtcgttt gaagtgattt cggcgctgct
     1921 gtcgcgctgc cgcgtttacg tgctgcagcc gttgacggag gagcaaatcg tgctcttact
     1981 gcagcgcgcg ctcgaagaca ctgagcgcgg tttgggcgag gcgcaccttg aagcggaaga
     2041 cgacgtcctg cgcaagattg cggcctactc cagcggggac gcccgcagcg cttacaacgt
     2101 gctggaagtc gcagcaacgc tggcggccga acagcagaag cagggagaga ggccccggct
     2161 caccgaaggc atcgtcaccg atgcgctgca aaagcgagtg ctccgatacg acaaagccgg
     2221 cgaagagcat tacaacctga tctccgcgat gcacaagagc gtgcgcaaca gcgatcccga
     2281 tgcggcgctc tattggctgg cgcgcatgat cgaggcgggc gaggacttgc tgtatatcgc
     2341 gcggcgcgtg gtgcgcatgg cctcggagga catcggtctc gccgatccca acgcgctggc
     2401 gatcacgctg gcagcgcgcg atgcggtgga ttttctcggc gtaccggagt ccaacctcgc
     2461 gctggcgcag gcggtaatct atctggcggt agcgccgaaa tcgaacgcgc tctacacggc
     2521 gtacggtgct gtgcaggagg atgtggagcg caccgcgcag gagccagtgc cgctgcattt
     2581 gcgcaacgcg cccactggac tgatgaaggc cctggattac ggcaagggat atcagtacgc
     2641 gcatgatc
//
LOCUS       DASRKK010000589         9183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_246673, whole genome shotgun sequence.
ACCESSION   DASRKK010000589 DASRKK010000000
VERSION     DASRKK010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246673"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFC15_13980"
     CDS             <1..546
                     /locus_tag="VFC15_13980"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZM11311.1"
                     /translation="ALVRLMAPTMCFTADEVWEMLPKVEGKPASVHLTHFPDAEELTG
                     ALPSGFDAAGLISEWSTLLTVRDEALKVLEVARADKQIGTGLEAQLHLSAPEPLYSLL
                     QKHRDQLRYLFIVSNVVLEKSAAGNGDTGLLITVRKAPGAKCERCWNYSIHVGDDKSY
                     PTVCERCSAVLAEIEATAETR"
     gene            674..1177
                     /gene="lspA"
                     /locus_tag="VFC15_13985"
     CDS             674..1177
                     /gene="lspA"
                     /locus_tag="VFC15_13985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZM11312.1"
                     /translation="MRKYFLLIAVSVVFMDRLAKWAVATYVPLHESLTVIPGFFHITH
                     VENPGAAFGLFAESSAPWKIAALVGFSLVALIIVSVLLWKNSHIMNTTTIGLSLILGG
                     ATGNLWDRIVDGRVVDFLHFYIGSYSWPDFNVADSAIVVGAVLLVSEILFAKSNDTVK
                     SRSLETH"
     gene            complement(1208..1564)
                     /locus_tag="VFC15_13990"
     CDS             complement(1208..1564)
                     /locus_tag="VFC15_13990"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HZM11313.1"
                     /translation="MFARPGILLRLEGAGIFGASLFAYHSLGGAWGIFLLLFLWPDLF
                     MLGYLANVQWGARLYNLAHTDAFPVLFGVVAYVMHKPAALLFAFIWLAHIGFDRMLGY
                     GLKYPTFFKDTHLQRV"
     gene            1676..4036
                     /locus_tag="VFC15_13995"
     CDS             1676..4036
                     /locus_tag="VFC15_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HZM11314.1"
                     /translation="MRFSWTALLAILLLAVVMDAQTPVKSDSIVSNSRIADGVEFRLH
                     HGTLRVQFCSDSLAHVTFRAASSAEHPQPWIVKTEWPAVPFTVTEDANKNTIIATKRL
                     RIVAEHNSAALVFEDMAGNLLVREAASPRPRELTPIAGDAEKSFRADAYFDLTQDEAI
                     YGLGQHQTGLLNQRGTDLLLMQDNTNISIPFLLSSRGYGLLWNSASLGHYENHFQPKL
                     ALRADADDAVDYYFIYGPEFDRIIAAYRTLTGPAPMLPRWAYGFWQSRLQYNSQQEML
                     DVAEKYRELKIPLDNLVLDFGWMVRMGSHQFTADFPDPAAMFSKLREMHVHTMISVWP
                     LYTPPSGNFDEMLAHNFFVTGGRTQVPSYYPGSRLFDAFNANARKTFWQQMKTGLYDK
                     GVEAWWLDSSEPLDFYGEEQGPMLEGAQTALGRGTRYANMYPLMETQAVYDGQRATTD
                     KQRVFILTRSAFLGQQRHAATSWSGDIAPTFDSLRRQIPAGLNYSMSGLPYWTTDIGG
                     FQGGDPNDPAYQEVYVRWFEYGTFCPIFRTHGARKANELWSYGPRAQEILTSYDKLRY
                     RLMPYIYSLAWKVTSEGYTPMRALVMDFPGDRSALDVPDQFLFGPAFLVNPVATAGAT
                     SRSVYLPSGAIWYDFWTGASLSGGETVRAAAPLETMPLYIRAGSIVPMDPELQYTSEK
                     PADLIELRIYRGADGRFTLYEDDGESYGYERGEHATISFTWTDASQTLSIGARSGSFP
                     GMVRERTFNIVLVGKGHGVGEAPVVSADRVVRYNGEAISVNFKSTR"
     gene            complement(4070..6394)
                     /locus_tag="VFC15_14000"
     CDS             complement(4070..6394)
                     /locus_tag="VFC15_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZM11315.1"
                     /translation="MSILSRLTTTSLAALLLLQGSGLPAQDSATPPKKKPSSTSSSTK
                     KKSATSAAAGQKKTPSKKGHAPRKKATAARTIRLHKSFVASSDLRPMARQLIEFRTPA
                     AYTGVESYATKHSGTGPGALAWFAIGYAHYLDAQYPASIAALEKSQAYIGELKDYTSF
                     FIGNSYVGSNNPQASLSYLKDFGTRYPDSVYANDAELAYVKALLATNHPAEAVQLLTH
                     RGGGGAEVEYLLGKAYVQNGQSRTGAEALRRVYYNYPTSSQADAAESDLKKIAGADSL
                     PAVTFAEHERRAEGLYKGRRWSPAADEYKLMVTLAPAGQQSQVNIQLANSLMKLGDNR
                     QAKEVLARIPDDGSEASAEKWYQSAEIARNTSDDAELTTILQHMRSTTPGSPWLESAL
                     FTAGNMYLLRQDYDRAIDYYREIHDRFPNSSKASYAQWKCAWLTYRQNRPEQAKKYFD
                     QQIEFYPGSTEVPNAIYWRGRMAEADQNYGLARAYYLKLTDRYRNYYYGVAARKRLAT
                     LPDAPAVTVASLQRIPGITKIEPESLQTTPPEDDLHYNRSRLLENAGALDLAVRELQA
                     GTSSGPSWEMVEIARMYTGAGEYYRALQALKHAISGYFAMDVSALPQPYWEGLFPRPY
                     WEALRRNSEANGLDPYLVASLIRQESEFNPGVVSHANAYGLMQLLPKTGKGTAKQVGL
                     HNYKTDSLLDPSINIELGTKYFREMVDQFGGQVEYALAAYNAGSSRVEDWRSAGNFHD
                     IEEFVESIPFTETREYVQAIVRNAEVYKQVYKTP"
     gene            6666..7295
                     /locus_tag="VFC15_14005"
     CDS             6666..7295
                     /locus_tag="VFC15_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM11316.1"
                     /translation="MGTIQPKVEEPASDELKLVHAAKAGDISAFEELVKRYDRNVFRI
                     AQHITQNREDAEDVVQDAFLKAYQNLSRFQEQSKFYTWLVRIAVNEALMRLRRRRPER
                     MVSLDEDIKTEDDSMPREVADWAPNPEQLYSQGELKEILGKTIQGLPPSFRTVFVLRD
                     VEGLSTEETADALDLSVPAVKSRLLRARLQLRERLTKYFKNRVGGDGKQ"
     gene            7292..7567
                     /locus_tag="VFC15_14010"
     CDS             7292..7567
                     /locus_tag="VFC15_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZM11317.1"
                     /translation="MTCSEFLKELTDYLDESMDAQTKAELEDHLQWCHNCYVVCNTTK
                     MTIEIYRESHLYELPDDLRGRLRTAIVSKCKGSGVVATAKSTTPEAK"
     gene            complement(8132..>9183)
                     /locus_tag="VFC15_14015"
     CDS             complement(8132..>9183)
                     /locus_tag="VFC15_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM11318.1"
                     /translation="PSAAPPVANSGQTTDAAQGTDSSAPANPAGNTSDSGTTTPNVKT
                     GSKDDVDAIGNRNVGKRGLGDWYSTDTEIKMGKQYAMMVEQTARMVQDPVINEYVNRI
                     GQNLVRNSDAKVPFTIRVIDSDEINAFALPGGFFYVNSGLILAADEEAELAGVMAHEI
                     AHVAARHAMQQMTRAQYAQMATIPLIFVGSWGIYEAASMAINLALPLTFLKFSRNFES
                     EADFLGVQYMYKTGYDPQAFVTFFEKIEAKNKKQPGTLARAFSSHPPTPERITKTQEE
                     IRRILPSQPEYVLDTSEFDSVKARLASIQNRKKALPNADPDKPTLRKTTADNSTTGTQ
                     QPADDRPTLSRRDDD"
BASE COUNT         1859 a         2537 c         2735 g         2052 t
ORIGIN      
        1 gcgctcgttc gcttgatggc tccgaccatg tgcttcacgg cagacgaggt ctgggagatg
       61 ttacccaagg tcgaaggcaa accggcgagt gtacacctta ctcactttcc agatgcggag
      121 gaattgacgg gcgctctgcc atcgggattc gatgcagcag gtttgatatc ggaatggtcg
      181 acattgctga cggtgcgcga cgaagcgctc aaggtgctgg aagtagcacg cgctgacaag
      241 caaatcggga caggcctgga ggcgcagttg catttgtccg cgccggaacc gctctactcg
      301 ctgctgcaga agcatcgcga tcaactgcgc tatttgttta tcgtctccaa tgtggtgttg
      361 gagaagtccg cggcgggcaa cggcgatacc ggattgctga tcacggtacg taaagccccg
      421 ggagcgaagt gtgagcgctg ttggaactac tcgatccacg tcggcgacga caaatcgtat
      481 cccacggtct gcgaacggtg cagcgcggtt ctggcggaaa tcgaagcgac ggccgagact
      541 cgttaacgga cggtcgcggc ggcagggctg cttctttcgg ccttagtccg cgtctaacta
      601 atgcatgaca ccggaacgac gtttcaaggt ctttgagttc aggacgaaag gtctgccaca
      661 atcgaacgat tccatgcgta aatacttcct ccttatcgcg gtgtcggtgg tcttcatgga
      721 tcggctcgcc aaatgggccg tggcaacgta tgttccgttg cacgaaagcc tcaccgtcat
      781 ccccggtttt tttcatatca cgcacgtgga gaaccccgga gcggcatttg gactgtttgc
      841 agaatcgtcc gctccatgga agatcgcagc gctggttgga ttctcgctgg tcgctctaat
      901 cattgtgtcg gtactgctct ggaaaaacag tcacatcatg aacactacaa cgattggcct
      961 gtcactgatt ctgggcggag ccacgggcaa tctgtgggac cgcatcgtcg atgggcgggt
     1021 ggtggacttc ctgcacttct acatcgggag ctacagttgg cccgacttca acgtggcgga
     1081 cagcgccatc gtcgtcgggg cagtcctgct ggtttcagaa attctctttg ccaaaagcaa
     1141 tgacacggtg aagagcaggt cgcttgagac gcattgactc gctcagggat ttggcgctgg
     1201 accacgccta cacccgttgc agatgtgtgt ctttaaagaa cgtcggatac ttcagcccat
     1261 accccagcat tctgtcgaac ccaatatgcg ccagccagat aaatgcgaat agcaatgcag
     1321 ccggcttgtg catgacgtag gcgacgacgc cgaacagcac gggaaatgcg tcagtgtgtg
     1381 ccaagttgta tagccgtgct ccccattgca cgttcgccaa gtagccaagc atgaaaaggt
     1441 cgggccacag aaacaacagc agaaatattc cccaggcgcc acccaacgag tgataggcga
     1501 aaaggctggc accaaagatg cctgcgccct caaggcgcag aagaattccg ggtcttgcga
     1561 acatgacggg tagatttcga tagcttactc ggggatgcaa acatttccgg tcggctgctc
     1621 gcaacaacca ctcggctaga atgtgtctga tagcgtcgcg atcttgctaa gacacatgag
     1681 attctcgtgg actgcactgc tggccatcct tctgctcgcc gtcgtcatgg acgcgcagac
     1741 gccggtcaaa tccgattcca tcgtttcgaa ctcccgaatt gcagatgggg ttgagttccg
     1801 gcttcatcat ggcaccctga gagttcagtt ttgcagcgat tcgctggcgc acgtgacttt
     1861 tcgcgccgca agcagtgcgg agcatcctca accctggatc gtcaagacgg agtggccagc
     1921 cgtcccgttc accgtcacgg aagacgctaa caagaacacc atcatcgcga ccaagcgcct
     1981 gcggattgtc gctgagcata attcggcggc gctggtattc gaagacatgg ccgggaatct
     2041 attggttcgc gaggcggcat cgccacggcc acgcgaactg acgccgattg ccggggatgc
     2101 agagaagagc tttcgcgccg atgcttattt cgacttgaca caagacgagg cgatttatgg
     2161 gcttggccag catcagactg gcctgctcaa tcagcgcggc accgaccttc tactcatgca
     2221 ggacaacacc aacatctcaa ttcccttcct gctgtcgagc cgcggatacg ggttactgtg
     2281 gaacagcgct tccctcggcc attatgaaaa ccatttccaa ccaaagctcg cgttgcgcgc
     2341 cgacgcagac gacgctgtcg attattactt catctacggc ccggaatttg accgcatcat
     2401 cgccgcatac cgcactttga cggggccggc gccgatgctt ccgcggtggg cttatggctt
     2461 ctggcaatcg cgcctgcaat acaacagcca gcaggagatg cttgacgttg cggagaagta
     2521 tcgcgagctc aagattccgt tggacaacct agtgctcgac tttggatgga tggtgcgcat
     2581 ggggtcccat cagttcacag ccgactttcc tgacccggcg gccatgttta gcaagctgcg
     2641 cgagatgcac gtgcacacca tgatttcagt gtggccgctg tacacgcctc ccagcggcaa
     2701 cttcgacgag atgctggcgc acaacttttt cgttaccggt ggacgcactc aggtccccag
     2761 ttactatccc ggcagccggt tgtttgatgc gtttaatgcc aatgcccgca agacattttg
     2821 gcagcagatg aagaccgggc tatatgacaa gggcgtggaa gcgtggtggc tcgactcctc
     2881 ggagccactc gatttctatg gggaggaaca aggccccatg ctcgagggtg cgcaaaccgc
     2941 gctgggcaga gggactcgct acgccaatat gtatccgctg atggaaacgc aggcggtgta
     3001 tgacgggcag cgagctacta cggataaaca gcgggtgttc attctgacca ggtcggcatt
     3061 tctcggccag cagcgccacg cagcgacctc gtggtcggga gatatcgcgc cgacatttga
     3121 ctctctgcgc cgccagattc cggcgggcct gaactattcc atgtcggggc ttccctactg
     3181 gaccaccgat attggcggtt ttcaaggcgg agaccccaac gatcctgcgt atcaggaagt
     3241 ctatgtgcgc tggttcgagt acggcacgtt ttgtccgatc ttccgcaccc atggagcgcg
     3301 caaggcgaat gagttgtggt cgtatggacc gcgggcgcaa gaaatcctga ccagctacga
     3361 caagctccgg tataggctga tgccgtacat ctattcgctg gcgtggaagg taacgtcgga
     3421 aggctacacg ccgatgcgcg cgctggtgat ggacttcccc ggcgaccgta gtgcgcttga
     3481 cgttcccgat cagtttctgt ttggcccggc ttttctcgtc aaccccgtcg ccaccgcagg
     3541 agccacttcg cgcagtgttt atcttccttc cggggccatt tggtatgact tttggacggg
     3601 agcatcgtta tcgggtggag agacggtgcg cgctgccgcg cctctcgaaa cgatgcctct
     3661 ctacatccga gcgggatcga tagttccgat ggatccggag ctgcagtaca ccagcgagaa
     3721 gccggccgat ctcattgaac tcaggattta tcgtggtgcg gatggcaggt tcactctcta
     3781 tgaagatgac ggcgagagtt acggctacga gaggggcgag cacgcgacga tttcattcac
     3841 gtggacggat gcatcgcaga cgttgtccat cggggccaga tctggcagct tcccggggat
     3901 ggttcgggag agaacattca acattgtgct cgtgggaaaa ggacacgggg ttggagaggc
     3961 gcccgttgtg agtgcggatc gggtggtgcg gtacaacggc gaggcgatct cggtgaattt
     4021 taagagcacg cgctagcagc agcgccagcg cacattctat tccggccctc tacggcgtct
     4081 tgtaaacctg cttgtacact tccgcgttgc gcacgatggc ctgcacatac tctcgagttt
     4141 cagtgaacgg gatcgactct acgaactctt cgatgtcgtg gaaattcccc gcagagcgcc
     4201 aatcctcgac ccgtgacgaa cccgcattgt aggcggctag agcgtattcc acctgtccgc
     4261 cgaattggtc caccatctcg cgaaagtact ttgtgcccag ctcgatgttg atcgagggat
     4321 cgagcagcga gtctgtcttg tagttgtgca agccgacctg cttggcggtt cccttgccgg
     4381 ttttcgggag aagctgcatc aggccgtagg cgttggcgtg agacaccacg ccgggattga
     4441 actccgactc ctgccggatc agcgaggcca ccaggtaggg atcgagacca ttggcttctg
     4501 agtttcgtcg cagcgcttcc cagtagggcc gcggaaacag accttcccag tagggttgcg
     4561 gcagagcgct tacgtccatt gcaaagtatc cgctgatggc gtgcttgagt gcctgcagcg
     4621 cccggtagta ctcgccggcg ccggtgtaca tgcgggcgat ctccaccatc tcccacgacg
     4681 gaccgctgct cgtgcccgcc tgtaattccc gcaccgccag gtcgagggca cctgcgttct
     4741 cgagcaatcg cgaacggttg taatgaaggt catcttcggg tggcgttgtt tgcaacgact
     4801 ccggctcgat tttggtgatg ccggggatgc gctgcaaact cgcaacggtc actgcaggcg
     4861 catccggcaa ggttgcaaga cgtttgcgtg ctgccacgcc gtagtagtag ttgcggtagc
     4921 gatcggtgag cttcaagtaa taggcgcggg ccagtccgta attctggtca gcctcagcca
     4981 tgcggccgcg ccaatagatg gcgttgggga cttcagtcga tccgggataa aactcaatct
     5041 gctgatcgaa atactttttc gcctgctccg gccggttttg gcgataggtc agccaggcgc
     5101 atttccactg ggcataagac gccttgctgc tgttgggaaa gcgatcgtgg atctcgcggt
     5161 aataatcgat ggcgcggtcg tagtcctggc gcaacagata catgttgccg gcggtaaaca
     5221 gcgctgactc cagccacggc gaaccaggag tcgtcgagcg catatgttgc aggatggtgg
     5281 tgagttccgc gtcgtcgctg gtgttgcggg caatttccgc gctctgatac cacttctcgg
     5341 cactggcctc gctgccgtca tcgggtatgc gggcgagcac ttctttcgcc tggcgattgt
     5401 cgcccagctt catcagagag ttggccagtt gaatattcac ttgcgactgt tgaccggcgg
     5461 gcgcgagggt gaccatcagt ttgtactcat cggcagcggg agaccagcgg cgtcccttgt
     5521 acaggccttc ggcacggcgc tcatgttccg cgaatgtcac tgctgggagc gagtcagctc
     5581 ctgcgatctt cttcaagtca ctctcggcgg cgtccgcctg agaactcgtt gggtagttgt
     5641 agtagacgcg gcgcaacgcc tcggctccgg tgcggctctg gccattctgc acatacgctt
     5701 tgcccaaaag gtattcgacc tcggcgccgc caccgccgcg atgcgtaagc aactggactg
     5761 cctccgctgg atggttggtg gcaagcaatg cttttacgta tgccagttcg gcgtcgttgg
     5821 cgtacaccga gtccggatag cgggtgccga aatctttcag gtagctcagc gacgcctggg
     5881 gattgttgct ccccacataa gaattgccga tgaagaacga cgtgtaatcc ttgagttcgc
     5941 cgatgtaggc ctgcgacttt tccagggcgg cgatcgatgc gggatactgc gcgtccaggt
     6001 aatgcgcgta gccgatagcg aaccaggcca acgctccggg tccagttccc gagtgtttgg
     6061 tggcgtagct ctccacgccc gtgtacgcag ccggagtccg gaactcaatc aactggcgtg
     6121 ccatgggacg caggtcgctc gaggccacaa aactcttgtg cagccggatg gttctggccg
     6181 cggttgcctt cttgcgcggc gcatgtccct ttttagacgg cgtcttcttt tggccggcgg
     6241 cagcggacgt tgcgctcttc ttcttggtgg atgaagacgt gctgctcggc ttcttcttag
     6301 gtggggtcgc ggagtcctgg gcgggcagtc cactaccctg cagcagcagc aacgcggcca
     6361 gacttgttgt ggtcagtcga ctgaggatac tcactgagtt tagtgtaacc cgattgcagt
     6421 ccgctgccgt ggaacgatgg cgttgtaagt tccggttccc tctcacggca tccgatggtg
     6481 tgcggcgtgc gtattctaaa atgtaagtga gtcgatgact tcggctcgat atcccgtgcg
     6541 ttcggtacaa agatgttgca atatgagacg atagtgggag tttcgaccaa gctgcagaat
     6601 ccgccttgta taatgttcgg gaactgaaag ttgtcaatac cgtaaaatcc tgaatctgca
     6661 aacacatggg aaccatccaa ccaaaagtgg aggagccagc tagcgatgag ctaaagctgg
     6721 ttcatgcggc gaaggccggc gacatcagcg cttttgaaga gctggtgaag cggtacgacc
     6781 gtaatgtgtt tcgcatcgct cagcacatta cgcaaaaccg cgaagacgcg gaagatgtgg
     6841 tacaggacgc atttttaaag gcgtaccaga acctgagcag gttccaggag caatctaagt
     6901 tttacacctg gctggtgcgc atcgcggtga atgaggcgct aatgcgcctg cgccgccggc
     6961 gtcctgagcg gatggtgtcc ctggacgagg acattaagac ggaagacgat tccatgccgc
     7021 gcgaagtagc ggactgggct ccgaatcccg agcaactgta cagccagggc gagttgaaag
     7081 aaattctggg taagacgatc caggggttgc cgccgagctt tcggacggtt tttgtattgc
     7141 gcgatgtcga gggtctctca acggaagaga ctgccgatgc cttggatttg agcgttccgg
     7201 ccgtgaagtc acgtttgctt cgtgcccggt tgcaactgcg cgaacgcttg actaagtatt
     7261 tcaaaaatcg tgttggtgga gatggcaaac agtgacctgc tctgaattcc ttaaagagct
     7321 aaccgactac ctcgacgagt cgatggacgc ccaaaccaag gccgaattgg aagaccatct
     7381 gcagtggtgc cacaactgct acgtcgtctg caataccacc aagatgacca tcgagatcta
     7441 tcgggaatcc cacctctatg aattgccaga cgacctccgc ggacgcctgc gcaccgctat
     7501 cgtcagcaaa tgcaaggggt cgggcgtggt cgctaccgcc aagtcgacta cgcctgaagc
     7561 gaagtgatcg actgacgggt acgctgcttc tattcgcgac tcgatcgctt tgtttttaga
     7621 aattccttct tccccgactg aacctttccg caccgtttcg aatccaacta ctcaacactg
     7681 ggtttgcata gatagttgct gcggcgccac gttctctgca acgaacttcg tcaactcgaa
     7741 ataacgcgga acggaggcga tgtatggggt tcttagatct gatgttcggt tgtgcacaca
     7801 agcggttcag tttccctatc acggtccggg gagcacggcg acgcgacagc ggagcggctt
     7861 cagtcactgg gacgtatgtg gtttgtctcg agtgcggaca cgaattaccg tatgactgga
     7921 agcagatgaa ggtggtcagg gaatcacgag cggccagggt cggagtcgcc caaccgcgca
     7981 gggccgcgta aattcggtcc ctggcgattc ttgccgttgc agtgtggctc aaacaccctt
     8041 cctgcgttcg gggagagcca tcttgattgt cccagcaaaa cgaaccggcg actcgcgccg
     8101 ccggtgctga gttctcgtct catattaact tctagtcgtc gtcacgacgc gatagagtcg
     8161 gccggtcgtc cgccggctgt tgcgtcccag tggtgctgtt gtcagcggtg gtcttgcgca
     8221 gcgttggttt gtcagggtcg gcattgggaa gggccttctt ccggttctgg atcgaggcca
     8281 gccgcgcctt caccgagtcg aattccgagg tgtcgagaac gtactccggc tgactgggca
     8341 ggattctgcg aatttcctcc tgcgttttcg taatacgttc cggggtcggg gggtgggagg
     8401 aaaacgctct cgccaaggtt ccaggctgct tcttgttctt cgcctcaatc ttctcgaaga
     8461 aagttacgaa tgcctgcgga tcgtaacccg tcttgtacat gtactggacg ccgaggaagt
     8521 ccgcctccga ttcgaagttg cgggagaatt tcaagaaggt cagcggcagc gcgagattga
     8581 tggccatgct ggccgcctcg taaatccccc agcttccaac gaaaatcaac ggaatcgtgg
     8641 ccatctgggc gtattgggcg cgtgtcatct gttgcatggc atggcgagca gccacatgag
     8701 cgatttcgtg tgccatgact cccgcaagtt ctgcctcttc atcggcagcc aggatcaggc
     8761 cgctattgac atagaagaac ccgcccggca gagcaaatgc gttaatctcg tcggaatcaa
     8821 tcaccctaat ggtgaaaggc accttggcgt cggaattgcg aaccaggttc tgtccgattc
     8881 gattcacgta ttcgttgatg accgggtcct gcaccatgcg ggccgtctgc tcaaccatca
     8941 tggcgtactg cttacccatc ttgatttcgg tgtcagtcga gtaccaatcg cctaaaccgc
     9001 gcttgccgac attgcggttg ccgatggcat cgacgtcatc cttgctaccc gtcttcacat
     9061 ttggggtcgt ggtgccgctg tcggaggtat tgccggccgg gttggcaggc gccgaagaat
     9121 cggtaccctg tgcggcatct gtcgtttgtc ctgaattggc caccggtggt gctgcggaag
     9181 gag
//
LOCUS       DASRKK010000590         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_540877, whole genome shotgun sequence.
ACCESSION   DASRKK010000590 DASRKK010000000
VERSION     DASRKK010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540877"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VFC15_14020"
     CDS             <1..1312
                     /locus_tag="VFC15_14020"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZM11319.1"
                     /translation="VPKHYDTASQMLGVTVNKILGPADRLLQSTAESIGVGDTFYRTS
                     VAIFQAPEGVLGGVTMPDPYFNGEGPERTTCKGCGGCMMGCRHGAKNTLDMNYLYLAE
                     KRGAQVFPETRVVDVQPLDDKADGSGGYKVSTVKSIAWINRHRRSFTCRAVVFAASSL
                     GTMELLFQLKDKQSLPLLSGQIGRYVRTNSESLIGVRVPGTTDDLSKGVAIGSGIYID
                     EHTHIEAVRYPNGSDAMSAWTTILTGGRPGPGRVALWLKNLLVSSIVHPIRTFRIMQP
                     FGWARESVILLCMQALDGHIDMCWQRSLFSPFRKTLVSRGQRIPTFIPQANEFALKMA
                     KAAGGSAMSMLPEILFDIPGTAHCLGGCVIADSTENGVVDYRNRVFGYKNMYICDGSV
                     IGANLGVNPSLTITALTERAMSFIPPASETTWNDDAEPVAVSVQ"
     gene            complement(1372..1920)
                     /locus_tag="VFC15_14025"
     CDS             complement(1372..1920)
                     /locus_tag="VFC15_14025"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HZM11320.1"
                     /translation="MNPARIVIKKYPNRRLYDTSASRYINLEDIGELIRKGKDVQVVD
                     AQTGEDLTRVTLTQVIVDDAKQQPTGLPLELLRQLIMSSDEVGREFIMWYLKSAYDAY
                     QNVQTKLQSGLTGVQSAAMSPISMIKNLIQGQSPENRAPESRADNTAELQLLRQRIAE
                     LEARGENPKPPKAPAKRKSRKK"
     gene            2031..2948
                     /locus_tag="VFC15_14030"
     CDS             2031..2948
                     /locus_tag="VFC15_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11321.1"
                     /translation="MKDNVKSYISPAQTPESSLVNMLSGWAQQGVENFFAAQRMLVDL
                     AMNQNATAINLVRERVENPKFSPVSILTELASEGMSNFIAGQKVLLDLVHQENEIVLN
                     GVRGRVSGSDAAVAMSDLVRRSLNTFVELQQDFLKIAGKQTHNWLTAVEKGKAYDGEQ
                     LVEMAREGMDHFVQTQQKFLNMIAEEASKATKSEAGRKAKVEELSELARQSSECFMEA
                     QKKLMDVTGKQMSASLKASGRALEMVKPLVAIPIPEMAREGVRSFVDTEKALIETMTK
                     RPAGHKVTARKGHSTKRTARTIKPEVVHA"
     gene            complement(3687..4226)
                     /locus_tag="VFC15_14035"
     CDS             complement(3687..4226)
                     /locus_tag="VFC15_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11322.1"
                     /translation="MRRRAEFFYQELDALQALHRAIRRDLVQESRKHNGAKLLCEIPS
                     IGPIRAALMTALIQTPQRFRTKRQLWTYSGLGIETHDSAQYRYVEGQLQRSKKPVQLR
                     GLNRNHNHDLKNVFKGTASWASSNPGPFQDFYVGLLGKGMKPEMARLTLARKIAAISL
                     RVWKRGEHFDAKHLKQQAA"
     gene            4128..>4347
                     /locus_tag="VFC15_14040"
     CDS             4128..>4347
                     /locus_tag="VFC15_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11323.1"
                     /translation="MFAALLDQISPYCSMQSLEGIEFLVEELGPTTHARFLNLFQPSR
                     TVARGVDRHAGASNGPASIERFESVHNPG"
BASE COUNT          910 a         1226 c         1254 g          957 t
ORIGIN      
        1 agtgcccaag cactacgaca ccgcgtcgca aatgcttgga gtcacggtga acaagatcct
       61 tgggccagcc gaccgcctgc tgcaatcgac cgccgagtcc atcggagtag gcgacacctt
      121 ctaccgcacc agcgtcgcca tcttccaggc gcccgaggga gtgctgggcg gagtgacgat
      181 gcctgacccg tacttcaacg gcgagggccc ggagcgcacc acctgcaaag gttgcggcgg
      241 atgcatgatg ggttgccgcc acggcgcgaa aaacaccctc gacatgaatt acctgtatct
      301 cgccgagaag cgtggcgccc aggtatttcc agaaacccga gtggtggatg tgcaaccgct
      361 tgacgacaaa gcggacggca gcggcggata caaagtctcc actgtgaagt cgatcgcctg
      421 gatcaatcgt catcggcgaa gttttacctg ccgcgcggtt gtctttgccg cctcatcgct
      481 tgggaccatg gagcttctct tccagttgaa ggacaaacag tcgctcccgc tcctgagcgg
      541 gcaaattggc cggtacgtgc gtaccaattc cgagtcgctg atcggagtac gggtgccggg
      601 tacaaccgac gatctgtcca aaggtgtcgc gatcggttcg ggtatttaca tcgacgagca
      661 cacccacatc gaagccgttc gctatcctaa tggatctgat gcgatgtccg cctggaccac
      721 gattctgacc ggcgggcggc ccggacctgg tcgagtcgct ctgtggctga agaacctcct
      781 ggtttcctcg atcgtccatc ccatccggac attccgcatc atgcagccct tcggttgggc
      841 gcgcgagtcg gtgatcctgc tgtgcatgca ggcactcgat gggcacatcg acatgtgctg
      901 gcagcgctct ctgttctcgc catttcgaaa gaccctggtc agccgcggcc aacggatccc
      961 caccttcatt ccacaagcca acgaatttgc cctgaagatg gccaaagccg ccggaggatc
     1021 cgcgatgagt atgcttccgg aaatactttt cgacatccct ggcaccgccc attgcctcgg
     1081 aggttgcgtg atcgcggact ctaccgagaa cggagtcgtc gactaccgca accgcgtctt
     1141 cggctacaag aacatgtaca tctgcgatgg ttcggtaatc ggagctaatc tcggcgtcaa
     1201 ccccagcctg accataacgg cgttgaccga gcgggcgatg agtttcattc cgccggcttc
     1261 ggagacaacc tggaacgacg acgctgagcc ggtggcggtc agcgttcagt aggactctgc
     1321 gatgtctgga ttggccgggc gtgaacagca gcgagcccgt tgatgctcgc tttatttttt
     1381 tcgggacttc cgcttggccg gcgctttcgg cggctttgga ttttcgccgc gcgcttccaa
     1441 ctccgcgatg cgctggcgca gtagctgtag ttcggcagtg ttatcggctc gactctcggg
     1501 cgcgcggttc tccggcgact gtccttgaat gagatttttt atcatgctga ttggagacat
     1561 cgcggcggat tgcaccccag tcagaccact ctggagctta gtctggacat tctggtaggc
     1621 gtcgtaagcc gacttcagat accacatgat gaattcgcga ccgacttcat cggaggacat
     1681 gatgagttgg cgaagcagtt ccaggggcaa tccggtgggt tgctgcttgg cgtcgtccac
     1741 gatgacctgg gttagcgtca cgcgggtgag gtcttcgcca gtctgggcat cgaccacttg
     1801 cacgtctttg ccttttcgga tcagctcgcc gatgtcctcc aaattgatgt accggctggc
     1861 ggaagtgtcg tagagtcttc gatttggata cttcttgatg acgatcctag ccggattcat
     1921 agtgacctgc cttcctgctg caacgcacaa ataacgtatg ccatattgac attctaccct
     1981 acaaggctta cattttagtt aatgctgcac tgcagcatac ggaggttgtc atgaaagata
     2041 atgtgaaatc atacatttct ccagcgcaga cgccggaatc gtcccttgtc aacatgctct
     2101 cgggatgggc gcagcagggt gttgaaaact tctttgccgc ccagagaatg ctggtcgatc
     2161 tggcaatgaa ccagaacgcg accgcgatca atctggtccg cgagcgggtg gaaaacccca
     2221 agttttcacc ggtctcgatt ctgaccgaac tcgccagcga aggcatgtcg aacttcatcg
     2281 ctggccagaa agtgttgctc gacctggtac accaggaaaa cgaaatcgtg ttgaacggcg
     2341 tgaggggccg ggtcagcggt tctgacgcag ccgtcgccat gtctgacctg gtgcggcgca
     2401 gcctcaatac cttcgtcgag ttgcagcagg acttcctgaa gatcgccggc aaacagacgc
     2461 acaactggct gacagccgtc gaaaagggca aagcctacga tggcgagcag ttggtcgaaa
     2521 tggcgcgcga ggggatggac cacttcgtcc agacccagca gaaattcctc aacatgatcg
     2581 ccgaggaagc ttccaaggca accaagagcg aagcgggcag gaaagcgaaa gtcgaggagc
     2641 tctccgaact ggcccgccag tccagcgagt gcttcatgga agcacagaag aaattgatgg
     2701 acgtaaccgg taagcaaatg agcgcgagcc tgaaagcatc cggccgcgcg ctggaaatgg
     2761 tcaagccgtt ggtggccatt ccgattccgg agatggcccg cgaaggcgtt aggtcctttg
     2821 tggataccga gaaggcactg attgaaacca tgacgaagcg tcccgccggg cacaaggtga
     2881 cggcccgaaa ggggcactcg acaaagcgga ccgcacgcac catcaaacca gaggtcgtgc
     2941 acgcttaatt gttctggttc tggctgtatc ggatggaacc atagtctgag tgagcaacgg
     3001 atgggatcca gttgctgtgc agtactgacg tgccgttgtc ctgggcagcg gtacgtcgtt
     3061 ttatgtttgt tgcttccagg gaaacagccc gttctgggcg ccggcacttg ctacatctcc
     3121 ccagccagga ggccggcatc aagcgggtga accggacctc aaagttaacc tgttcgtagc
     3181 aagttaaaat gtccgctttt tgtagcaagt gaaatgtccg gttttaggtt ttcttatacg
     3241 ctagccgtcg tcgctgtggg aaagtcctat agaaggggat gtcaagagac atttttctcc
     3301 cttcgccaga cgcggtagaa tttctcgcgc atccagccct ccccctcgcg ggttgcctct
     3361 tcgtctggac ggcttttcgc tgcggagcgt cagaccgagt tgttcccatt gcgacggttt
     3421 catgacacga tccgttgtcg acgccattcg gctcctgaaa cttgcaagca gagcgggcag
     3481 gcaaagtgcc aaccatggtc ctatctgaga ctcgaggcct atagctttct tgggttatcc
     3541 ggaagggcat agggtggaaa ctgagactga gtgccgaggc acaggcctac tttccccctg
     3601 gatactctct tccgaacccg agcgtcgcaa gaaccgactg gccaacatag ggcatgaaac
     3661 cctttgggcg tggcttcctc agacgctcaa gctgcttgct gttttagatg ttttgcgtcg
     3721 aaatgctctc ctctcttcca aactctcaag ctgatggcag cgatcttgcg ggccaaggtc
     3781 agacgagcca tctctggctt catcccttta cccaacagac cgacatagaa atcctggaac
     3841 gggccgggat tactactggc ccagctggcc gtgcctttga acacgttttt cagatcatga
     3901 ttgtggttgc ggttgaggcc acggagctga actggtttct tggaacgttg gagctgtcct
     3961 tccacgtagc ggtattgcgc actatcgtgg gtctcgatcc caagaccgct gtacgtccac
     4021 agttgtcgct tggttcggaa acgttgaggg gtttggatga gcgcggtcat cagagcagct
     4081 cgaatcggac cgatcgaagg aatctcacac agcaactttg ccccgttatg tttgcggctc
     4141 tcctggacca gatctcgccg tattgctcga tgcaaagcct ggagggcatc gagttcctgg
     4201 tagaagaact cggcccgacg acgcacgcca ggttcctgaa tcttttccag ccatcccgca
     4261 cggtggcgcg gggcgtagac cgacatgccg gcgcaagcaa tggcccagct tcgatagagc
     4321 gctttgagtc ggttcataac ccgggtg
//
LOCUS       DASRKK010000591         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_737247, whole genome shotgun sequence.
ACCESSION   DASRKK010000591 DASRKK010000000
VERSION     DASRKK010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737247"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..850)
                     /locus_tag="VFC15_14045"
     CDS             complement(215..850)
                     /locus_tag="VFC15_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11324.1"
                     /translation="MKNAHERFGKLVYRRRTEKTATNLRVFLDAFVGEHRGKAHLVSV
                     VGGDVEIGALAAAFANGDSFTAVDPYGEESIVSLGEKPSCFRGSIVVPGRRRPLRHLV
                     SCSQELADTTSDGKLLLISDDQSFIWSSLVRHYGLPATPEWGPWMISQLQQQKRIQPL
                     LGFGYAGVAVKAKRKELLALLRKGLRSNRLAFPFQNGAVEWPENQLIKKTA"
     gene            complement(1078..1470)
                     /locus_tag="VFC15_14050"
     CDS             complement(1078..1470)
                     /locus_tag="VFC15_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM11325.1"
                     /translation="MSALATDYGSLLSETKPEVIHDEGQNDTFVARLEELTSKQVVTP
                     AEEKLIELLTVLIENFEAKHYPVQGSGPLDVIRHLMEENHLRQKDLVDVFGTESIVSD
                     VLNGKRDLAKDHIRKLSERFGVSPALFF"
     gene            complement(1467..1754)
                     /locus_tag="VFC15_14055"
     CDS             complement(1467..1754)
                     /locus_tag="VFC15_14055"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HZM11326.1"
                     /translation="MHVISKKAWKDAVAADPALEGPVSEWYKVASSASWQNLMDVRKT
                     YSHADFVDPYTVFNIKGNNYRLVVKIEYRWQLIFVKHLLTHAEYERGGWKK"
     gene            complement(1927..2187)
                     /locus_tag="VFC15_14060"
     CDS             complement(1927..2187)
                     /locus_tag="VFC15_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11327.1"
                     /translation="MTLKVGIREFRDKLASYLLESEEPVAITRHGDIVGYYVPARRKR
                     SETERATLKVAAAHLQQALAAEGISAEEILRDFKRWHRDHRK"
BASE COUNT          487 a          666 c          622 g          568 t
ORIGIN      
        1 ctgattacct caactgtgtc gtcgccaatg cacccgggcc ggtgtttgcc gttcatgcct
       61 ccgcacgacg cacaaatgcg atcgcctgta tgtagcaatg ccatgggttc aggtcctccc
      121 agaactgttc agaatgtggg tgtgtagtgc atccgcgagt ggccggtctc cggttctccg
      181 cagcagtcgg cgaaggccgt cgttcacgcc ggaatcaggc agtcttcttg atgagctggt
      241 tttcgggcca ttcgacggcc ccattttgga aaggaaaagc gagtcgattg ctgcgcagcc
      301 ccttccgcag cagcgccaat aactctttcc gcttggcctt gacggctact ccggcatatc
      361 caaacccgag aagaggctga atacgctttt gctgctgcaa ttgcgagatc atccacggtc
      421 cccattctgg cgtggcgggc aagccgtaat gacgcaccag cgaagaccag atgaaggatt
      481 ggtcgtcgct gatcagaagc agtttcccat cggaggtggt gtccgccaat tcctgcgagc
      541 aactcacaag atgtctcagc ggccgtctcc gtccgggaac cacgatggag ccgcgaaagc
      601 atgacggctt ctcgcccagc gagacgatac tctcttcacc gtaaggatcg accgcggtaa
      661 atgagtcacc gttggcaaag gcggcagcca gggcgccgat ctccacgtcg ccgccaacca
      721 ctgagaccaa gtgggctttg cctcggtgct cacctacaaa ggcatccagg aaaacgcgaa
      781 ggttggttgc cgtcttttct gtgcgccgcc gataaacgag tttgccgaag cgttcatgag
      841 cgttcttcat cgccgtgtgc acctctttcc ctgatttagt tcagcgactg gaccggcttg
      901 gccagcgccg tttttcggct agggtgttca aaggactgaa ggtttgtaca tcgaggaatc
      961 ttgggcaggg aggtacagag atgttcgatt acggagtgct tattgggtga tcgtcaggcg
     1021 atggcagtct tcgcaaccga gttgggggaa caggctggcg gcgaaagttg agcaacctta
     1081 gaaaaagagc gctggagata ctccgaatcg ttcgctgagt ttgcgaatgt ggtcctttgc
     1141 cagatcgcgc tttccattga gtacatcgga cacgatgctc tccgttccaa agacgtccac
     1201 caggtctttc tggcgcagat ggttttcctc catcaggtgc cgtatgacat caagtggacc
     1261 ggaaccttga accggatagt gctttgcctc gaagttctcg atcaaaaccg tcaagagttc
     1321 gatcagcttc tcctccgccg gagtgacgac ttgcttggaa gtcaattcct cgagtcgcgc
     1381 cacgaacgtg tcgttctgtc cctcgtcgtg aatgacttcg ggcttcgtct ccgaaagcag
     1441 agatccataa tcagttgcta gcgcactcat ttcttccatc ctccccgttc gtattccgca
     1501 tgggtaagca gatgcttaac gaagatcagc tgccaacggt actcgatctt cacaaccagg
     1561 cgatagttgt tgcccttgat gttgaagacc gtgtacggat cgacgaagtc ggcgtggctg
     1621 tatgtctttc gaacatccat cagattctgc cacgatgcgc tgctcgccac cttgtaccac
     1681 tccgagactg ggccctccag cgcgggatcg gctgcgacgg catctttcca cgccttcttg
     1741 ctgatcacat gcattgactt ggcctacttc gcatattgcg aaggttcata atacttcgca
     1801 tttcgcgaag atgcaattgg ataccgtaag tatttaagaa cataggcttt ctagtcccgt
     1861 aaatgtatag tcgaaacccg caaaaccgcg atttgagtca cggcgagcct attcaagaat
     1921 ctgccatcac ttgcgatgat cacggtgcca gcgtttgaag tctctcagga tctcttccgc
     1981 ggagatccct tctgcagcga gcgcttgctg caagtgcgcc gcggccacct tgagcgtcgc
     2041 tcgttccgtt tctgagcgct tccgccgagc aggaacgtag taacccacga tgtcaccatg
     2101 acgcgtgata gcaaccggtt cctcagactc taagagatag ctggcaagct tgtcgcgaaa
     2161 ttctcgaatg ccgactttta gggtcatgat tctacgctcc caaaagcgta ccgttgtgta
     2221 cgcaagtctt tcacctccgg cgttccttca gcaagcactg agtcgcggcg tgctcccggt
     2281 ctatttgctc tgaacctcct ccttggtctc cttcaactcg atgatctggc cactctcata
     2341 ggc
//
LOCUS       DASRKK010000592         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_149775, whole genome shotgun sequence.
ACCESSION   DASRKK010000592 DASRKK010000000
VERSION     DASRKK010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149775"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFC15_14065"
     CDS             <1..475
                     /locus_tag="VFC15_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZM11328.1"
                     /translation="FYLSWKAKQFDFRTRFIELAGEVNTNMPYFSVEAVASALNKEKK
                     ALNGSKILVLGLSYKKDIDDLRESPSLTIIELLQKRGAMVSYNDPYFPTVGHGRRYAL
                     NMTCAPLENLDQYDCVVIVTDHSDYDYPSIVREAKLVVDTRNATKGIDSPKIVRC"
     gene            complement(726..1187)
                     /locus_tag="VFC15_14070"
     CDS             complement(726..1187)
                     /locus_tag="VFC15_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZM11329.1"
                     /translation="MALVLKSPAFAHGAEIPKKHSCEAADVSPALEWDGSPARTISFA
                     LIMDDPDAPAGTWVHWVLWNLPASAHELSEAVAKQDQLDDGTRQGRNSFRKIGYNGPC
                     PPPGKPHRYFFRLYALDEKLDLAPGANSSDLQEAMKGHIIIQAEYMGTYRR"
     gene            complement(1381..2313)
                     /locus_tag="VFC15_14075"
     CDS             complement(1381..2313)
                     /locus_tag="VFC15_14075"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HZM11330.1"
                     /translation="MISSRYRGPSTPCPVATAPGLVAQDDSRIGVATHIASTHNGRQK
                     ALVIPREHGAWGMLLVPLATGAVAALRSSVNGGALALFIVAAMSLFWLRTPVESWLDT
                     SPIKAQTKDERAFVLKAIAGIGVVAAVSIAALMWGGQVRGLLTIGAVAGLAFAIQAGV
                     KKLGRKGRMPAQIIGAVGLTSTAAGAYYVATGKLDHIAVELWLANWLFAGDQIHFVQV
                     RIRSSRAANMDEKMKQGLPFFCGQVGLIGVILAACRFGLFSDAMALAFLPVLVRGTLW
                     FVRRYRPLDVHKFGFSELTHSLIFGALLCASFLV"
     gene            complement(2513..3763)
                     /locus_tag="VFC15_14080"
     CDS             complement(2513..3763)
                     /locus_tag="VFC15_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM11331.1"
                     /translation="MSESTGSTHLAATKQYTPRLVFWEVTKGCNLRCIHCRATATELM
                     SPADLPTEKALNIISQIADFGSPILVLSGGEPLYRADIFQLAEYATGRGLRTALATNG
                     TLVTKDVAQKIKQAGIKRVSISLDGSDAHTHDSFRGIPGAFDAAVYGMRNLQELGVSV
                     QINTTIARHNAHQLPAVLDLARTLGADALHTFLLVPVGCGVDIAAEQMVAPEEYERML
                     NWFYDQSLRGGIELKATCAPHYFRVARQRRAADRMAASAEAPTPVPSVHDIGPTDMTM
                     PGSTGINLKPGGGPPVGHTGHPGGHPGDMNAMTKGCLAGTGVCFISHEGEVFPCGYLP
                     AIAGDLRKQSFAEIWNDSDVFAQLRDDDNLKGKCGCCEFRHVCMGCRARAYAATGDFL
                     AEEPFCVYEPGTSNQMIRAAAIKR"
BASE COUNT          815 a         1175 c         1163 g          764 t
ORIGIN      
        1 gttttatctg tcgtggaagg cgaagcagtt cgattttcgt acccgcttca tcgaactggc
       61 cggcgaagtg aacaccaata tgccgtactt ttccgtggaa gccgtcgcca gcgcgctgaa
      121 caaggagaag aaggcgctca atggctcgaa gattctagta ctggggcttt cctacaagaa
      181 ggacattgat gacctgcggg aatcgccgtc gctcacgatt atcgaactgc tccagaagcg
      241 cggcgcaatg gtcagctaca acgatcccta cttcccgacg gtggggcatg gacgccgcta
      301 cgccctgaac atgacgtgtg cgccgctgga gaatttggac cagtacgact gcgtggtgat
      361 cgtcaccgac catagcgact atgactatcc gagcattgtg cgggaggcga agctggtggt
      421 ggatacgcgc aacgcgacca aagggatcga ctcgccgaaa attgtgcgct gctaacctgg
      481 atttatccat gaacttgaag atgtcatcct gagtgttaca tgggcctgcg acccacctat
      541 agggatgaaa atcaaccaca gagatcacgg agatcacgga gaaaactcga tagaaaccaa
      601 acctccgtgt cctccgtgtc ctccgtgccc tccgtggtga atgactttcg acgaagcgaa
      661 gcacccgcaa agacttccga cccacatcct ttgcgggccg tgcgggcgcg cagggataca
      721 tcgcgctatc gccgataggt tcccatgtac tccgcctgga ttatgatgtg gcccttcatg
      781 gcttcttgaa gatctgagct gttggcgcca ggcgcgagat cgagtttttc atccagggca
      841 tacaagcgga agaaataccg gtgcggcttg cccggcggcg gacacgggcc gttatagcca
      901 atcttcctga agctgttgcg cccctgccgc gtcccatcgt ctaactggtc ctgcttcgca
      961 actgcctcgg aaagctcatg cgcgctcgcc ggcaggttcc acagtaccca gtgaacccac
     1021 gttccggcag gtgcgtcggg gtcatccatg atcagcgcga agctgatggt gcgcgccgga
     1081 gacccgtccc attccagtgc cggcgaaacg tccgcggcct cgcaggaatg tttcttcgga
     1141 atctctgcgc cgtgcgcgaa tgcggggctt ttgagcacaa gtgccataag tgaacgacct
     1201 cctgtagatg gatgtgactg tgacaccgtg ctgccgccaa ctgggcccag caccagcacc
     1261 atcgcggcga gttttgcgat tgaacgaagg tgattcagac gggaagctgc ggaagcgttg
     1321 ctcggggtga tcggcatcat ggtacgcaat gccaaacgat atcagaacag cggatgaaga
     1381 ttacaccaag aacgatgcgc acagcaatgc gccgaagatc aaggagtgcg tcagttcgct
     1441 gaagccgaac ttgtgcacat cgagagggcg ataacgccgg acaaaccaca gagttccgcg
     1501 aaccagaacg ggtaggaagg ccaatgccat ggcatccgag aagagcccga agcggcaggc
     1561 ggcgaggatt acgccgatga ggcccacttg tccgcagaag aagggcaatc cctgtttcat
     1621 cttctcgtcc atgttggcgg cgcggctgga gcggatgcgt acctgcacga agtgaatctg
     1681 gtcgccggca aacaaccagt tggccagcca caactcgacc gcaatgtggt ccagtttgcc
     1741 cgtggcaacg taatatgcgc ctgccgcagt ggaggtaaga ccgaccgcgc cgatgatctg
     1801 cgccggcatc ctccctttgc gccccagctt tttgacgcct gcttgtatcg cgaaagctaa
     1861 acctgcaacc gcgccgatcg tcagcagtcc gcgaacctgt ccgccccaca tcagcgccgc
     1921 tatgctgaca gcggccacca ctccaatgcc ggcaatcgcc ttcagcacga aagcgcgctc
     1981 gtctttggtc tgtgccttga tcggcgaggt gtccagccaa ctctcgaccg gcgtgcgcag
     2041 ccagaagagc gacatcgctg caactatgaa gagcgcaagc gcgccgccgt tcacactgga
     2101 gcgtagcgcc gccaccgcgc cggtcgcgag cggcaccagc agcatgcccc acgcgccatg
     2161 ctcgcgcgga atcaccaggg ctttctgtcg tccgttgtgg gtcgaagcga tatgggtggc
     2221 taccccaatt cgactgtcat cctgagcgac gagtccgggc gccgtagcga ccggacaagg
     2281 agtcgaagga cccctatacc tggaagaaat cacagtgcgg acgcctggta caggaacaag
     2341 tctgggattg cttggcggcc aaaggggttc ttcgactcct cgctccaaca ccccagcaag
     2401 cacaaacccg gtgcttgctg gggaccccgt ttgtgctcgt cgctcagaat gacacctcaa
     2461 caaattacgc tcagtcatta aataatccag cgaaggcccg cactacgcaa cactatcgct
     2521 tgatcgcggc ggcgcgaatc atctgattcg aggtgcccgg ctcatagaca cagaacggct
     2581 cttccgccag gaagtcaccg gtggcagcgt aagcccgggc gcggcagccc atgcagacgt
     2641 ggcggaactc gcagcagccg cacttccctt tcaggttgtc gtcgtcgcgc aactgggcga
     2701 agacatcgga atcgttccag atttcagcga agctctgctt gcgcaaatca cctgcgattg
     2761 ccggcagata tccgcaggga aaaacttcgc cctcgtggga gataaagcaa accccggttc
     2821 ccgccaggca gcccttggtc atcgcgttca tgtcgccagg gtgcccgcca ggatggcccg
     2881 tgtgtcccac tggcggcccg ccacccggct tcaggttgat gcctgtcgaa cccggcatgg
     2941 tcatgtccgt cgggccaatg tcgtggacgc tgggcacggg tgtcggagct tcggcgctcg
     3001 ccgccatgcg gtcggcggcc cggcgctgcc gcgccacccg gaagtaatgt ggggcgcagg
     3061 ttgccttcag ttcgatcccg cctcgcagcg actgatcgta aaaccaattc aacattcgct
     3121 catactcttc cggagctacc atctgctcgg cggcgatgtc tactccgcat cccacgggca
     3181 ccagcaggaa ggtatgcagc gcatcggcgc cgagggtgcg tgccaggtcg agcacagccg
     3241 gcaactggtg cgcgttgtgg cgcgcaatcg tggtgttgat ctgcaccgaa actcccagct
     3301 cctgaaggtt gcgcatgcca tagacagcgg cgtcgaacgc gccgggaatg ccgcggaacg
     3361 aatcgtgagt gtgcgcatcc gatccatcca gagagatcga gacccgtttg attccggctt
     3421 gcttgatctt ttgcgcaacg tctttcgtca ccaaggttcc gttggtggcc agcgcggtac
     3481 gaagaccgcg gccggtcgca tactctgcta gttggaaaat gtccgcccgg tacaggggtt
     3541 ctccgccgct gagtaccagg atcggactgc cgaagtcggc aatctgcgaa atgatgttaa
     3601 gcgctttctc ggtcggcaga tcggcggggg acattagctc ggtcgcggta gcacggcagt
     3661 ggatgcagcg caggttacag cccttggtaa cttcccagaa aaccaggcgc ggcgtgtatt
     3721 gtttggtggc cgccagatga gtgctgccgg tgctttcgct cattgttgtt gtcctccccc
     3781 gctcttgcgg aattcggctc tactgtatgt atccacgata ggattttcac gacccggagg
     3841 gccgtgagtc ttatcaccag cccctgtgat atgcgtcact tggaggattt gggacgggga
     3901 cacagcacga gtggtta
//
LOCUS       DASRKK010000593         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_247109, whole genome shotgun sequence.
ACCESSION   DASRKK010000593 DASRKK010000000
VERSION     DASRKK010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247109"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VFC15_14085"
     CDS             <1..1053
                     /locus_tag="VFC15_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZM11332.1"
                     /translation="PTRKGFGRSLQNNGDDERVVCLGLDISGSITISDFYSGKPERKN
                     RWISMGIAEQSATAAAAGLAREGKLPVFGTYGTFAAARNLDQIRVSICYGNFNVLIAG
                     AHGGVSVGPDGATHQALEDLFAMCGLPNMSVVVPCDAIETRKATDYLLLQHVGPKYIR
                     FAREATPIVTRENTPFVFGVANVSRLRREAPEMIDAFETVLAPNYENEHEDLSIIACG
                     PMVPEAMRAAWILKQEFGYETRVVNMHTMKPIDETAIVRAAKETGVVVTAEEHQIGAL
                     AWRVSGVITESPELLGTPVITGAIGVKDRFGDSGAPWELIKEFEVSAEHIAQKAVELI
                     AIAKMKKEEDRFQLAH"
     gene            complement(1452..>2187)
                     /locus_tag="VFC15_14090"
     CDS             complement(1452..>2187)
                     /locus_tag="VFC15_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM11333.1"
                     /translation="TSAIASKKGLFEVADRGTLFLDEIGTMNLETQSKILRVLQDRKF
                     MHLGGVSEIQVDVRIIAATNVNLQQQVKDGKFRDDLFYRLNVITIDLPPLRQRKNDIP
                     MLVEYFIAKFAEENERLPLRMTPEAMRPLLEYDWPGNVRELENVIERAVVLATGPDLS
                     VDLLPDNLIGRGSKFAMMEHRPDASLFEIIEDCERRIISDMLEKCGWNQTEAADHFRI
                     PLSTLNQKIKRLNIEIKKKSRENGNS"
BASE COUNT          468 a          613 c          668 g          438 t
ORIGIN      
        1 cccacgcgca aaggcttcgg gcgatcgctg caaaacaacg gcgatgacga gcgggtagtc
       61 tgtctcggcc tcgacatttc cggttcgatc accatcagcg acttctattc tggcaaaccg
      121 gagcgcaaga atcgctggat cagtatgggc attgcggaac agtcggcaac cgcggccgct
      181 gccggactgg cgcgggaagg gaagttgccg gtcttcggga cctacggaac gtttgccgcg
      241 gcacgcaatc tggaccagat tcgggtttcc atctgctacg gaaatttcaa tgtattgatc
      301 gccggagctc acggcggagt ttcggttgga cccgatggcg ccacgcacca ggcgctggaa
      361 gatctgttcg ccatgtgcgg actgccgaac atgtcagtcg tagtgccctg cgacgccatc
      421 gagacgcgca aggcgaccga ctatctgctg ctccagcatg ttgggcccaa gtacatccgc
      481 ttcgcgcggg aagccactcc gattgtcact cgcgaaaaca cgccatttgt atttggagtg
      541 gcgaacgtga gccgtctccg ccgcgaagcg ccggaaatga tcgatgcgtt cgaaaccgtg
      601 cttgctccca attatgaaaa cgagcacgaa gacctgagca taatcgcctg cgggccgatg
      661 gtgcccgagg ccatgcgtgc ggcttggata ctgaagcagg agtttggcta cgagacccgc
      721 gtggtgaata tgcacaccat gaagccgatt gatgaaacgg ccattgtgcg ggcggccaaa
      781 gagacgggcg tagttgtcac cgccgaggag caccagattg gcgccctggc gtggcgcgtg
      841 agcggcgtga tcactgaaag tcccgagtta ttgggaacgc cggtgattac gggcgccatc
      901 ggcgtaaagg accggttcgg cgattccggt gcgccgtggg aactgatcaa ggagttcgaa
      961 gtcagcgccg agcacatcgc gcagaaggcg gtggagttga tcgccattgc caaaatgaag
     1021 aaggaagaag atcgcttcca actggcgcac taaagcggtt ctagagttgg acgctctgag
     1081 ggccggctct atgtgagaac gaaccttggt attcgccagt ggaatgtgtt ggcggaaaat
     1141 ccagcgccgt aggcgcggaa taatctagcc catcagcacg cagccgcagg cggagtgctg
     1201 ggtaaagtgg gaaataaatc cgagtcccgc agggacggca gaggttccca caccgacgta
     1261 caagcaagcc gcagcgagcg cgaagattga caggcaccac tgataaagtc agaaagcaag
     1321 tctgtcgtcg gctggaagcc gactccatcc gataaacaaa ccgtgcggac gcggaccctt
     1381 cggctgcgca cagggcaggc tctaaaggtc cgcactaccc aaactacaac caggctggaa
     1441 ccgcactgcc gctagctgtt cccgttttcc cggctcttct tcttgatctc aatattcaga
     1501 cgcttgattt tctggttcag agtggagagc ggaatgcgga agtggtcggc ggcttcagtt
     1561 tggttccagc cacatttttc cagcatatcg ctgatgatgc ggcgctcgca gtcttcaatg
     1621 atttcgaaca gcgatgcgtc ggggcggtgc tccatcatgg cgaacttgct gccgcggccg
     1681 atgagattgt ccggcagcag gtccacggag aggtccggcc ccgtcgccag cactaccgcg
     1741 cgctcgatca cgttttccag ttcgcgcaca ttgcccggcc agtcgtattc cagcagcgga
     1801 cgcatcgctt cgggagtcat gcgcagcggg agccgctcat tctcctcggc aaacttcgcg
     1861 atgaaatact ccaccagcat tggaatgtca tttttgcgct gacgcaacgg aggcaggtca
     1921 atcgtgatta cgttgaggcg atagaacagg tcatcgcgaa actttccgtc tttcacctgt
     1981 tgctggagat tgacattggt ggcggcaata atgcgcacgt ccacttgtat ctcgctgacg
     2041 ccgcccaggt gcatgaactt gcggtcctgc agcacgcgca ggattttgga ctgcgtctcc
     2101 aggttcatgg tgccaatctc gtcgaggaac agcgtcccgc gatcggccac ctcgaacagg
     2161 cctttcttgc tggcgatggc gctggtg
//
LOCUS       DASRKK010000594         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_638860, whole genome shotgun sequence.
ACCESSION   DASRKK010000594 DASRKK010000000
VERSION     DASRKK010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638860"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFC15_14095"
     CDS             complement(<1..257)
                     /locus_tag="VFC15_14095"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZM11334.1"
                     /translation="MDSFIIAGRQFRSRLIVGTGKYKSGPETARAIAASGAEMVTVAV
                     RRVNLDRSKESLLDFIDPKQYFLLPNTAGCYTADEAIRTARL"
     gene            complement(441..1739)
                     /locus_tag="VFC15_14100"
     CDS             complement(441..1739)
                     /locus_tag="VFC15_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11335.1"
                     /translation="MDLNGTLRSFAEFIQNLDCSGQFDEGFFKDSTVLMTCGDHTVIL
                     IGEDADKYTEMVSSIYMAVSERRPVSKRAVSGILDDFFRRMLSSGDRTDNPSLAKQVE
                     DEMQGLKNALFEQPKNWEVRLIVDGLASSGLPMNVGQVQFQYLEEAAFSNLQNQVLDA
                     VRNVGVKNSDDAASYLESSLKLLQGKTIGVLSVNAVDGEAAILAARHKLQTTIDAINF
                     FSSREALGGWAFLPGDTMPQREFVLAVCEDGRVTPSFRQAGPIRRIPLDQIARRNGFP
                     RVSDMLGEEVPTDLEERILASIQWAGPAQVEARREEAFLLYAIALESLLLGRDVKTEI
                     SHRLAVRCAHLGGGPTMADKKRVVEQILSLYQVRSNIVHSGNFLVSDAEIGLIREYCV
                     LTLLIVIDTEPFRSMGKVKELEQWFQTQLLSGGISPTAAG"
     gene            complement(1807..2610)
                     /gene="tatC"
                     /locus_tag="VFC15_14105"
     CDS             complement(1807..2610)
                     /gene="tatC"
                     /locus_tag="VFC15_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZM11336.1"
                     /translation="MPSPIVETAQKRASEEMTGMSFLQHLEELRRRIIYSFLYVVAGF
                     CVCWWFHEQIFTIMQKPIVTALANHKMDTQLVYLNPTDPFNMYLKIAFLAGIFVASPF
                     VLYQVWAFIAPGLYRNERRYVFPFMFSTVGLFLAGGFFGYKMVYPAALDFLIGWSAQF
                     KPMITVGEYTDLFLTIIAGLGIVFEMPILVFFLSLMGILTAGWMWRNFRYSILVIFII
                     AAIITPTTDIMNMCIFAAPMIVLYLFSIAIAWFVHPTQRKKRAAKAETK"
     gene            complement(2603..2989)
                     /gene="tatB"
                     /locus_tag="VFC15_14110"
     CDS             complement(2603..2989)
                     /gene="tatB"
                     /locus_tag="VFC15_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZM11337.1"
                     /translation="MNLGMPEMIFIFLLALVVVGPKRLPGLARQMGKWVAEFKRASND
                     FKNQLETEMMNIELDERAKKQTEAAEALPSEKSWEATILPPVGIVSRGSADAISQLET
                     DPAPAELPAAPSMNEPPTPARSTPDA"
BASE COUNT          768 a          998 c          944 g          768 t
ORIGIN      
        1 aggcgcgcgg tgcggatcgc ctcgtcggcg gtgtagcagc cggcggtgtt gggcagcaga
       61 aaatattgct tgggatcgat gaagtcgagc agcgactcct tgctgcggtc gaggttcacg
      121 cggcgcaccg cgaccgtgac catctcggcg ccgctggccg cgatggcgcg cgccgtctcc
      181 ggccctgatt tatatttgcc cgtgcccacg atgagccgcg agcggaactg cctgcctgca
      241 atgatgaatg agtccatggt catattgtaa tgaaaggcgg aggtgtgtgg gtgtgactgg
      301 tcaaggtgtc agctatagct gtcagcactc gctaatcgga ttgcgcaaac gggaagttcc
      361 ttccagttga aatggtcact gatcatttgc aaattgcaaa ccgctgatca atgttattag
      421 gtaaccaaac gtccggtcgc ttaacccgcc gcagttgggc taatccctcc ggacaacagt
      481 tgcgtctgaa accactgttc gagctcttta accttgccca ttgatcgaaa cggctctgtg
      541 tcgattacta tgaggagagt caaaacgcaa tattcccgaa taaggccaat ctctgcatca
      601 ctgaccaaga agtttccaga atggacgatg tttgatctca cctgatacaa ggacagaatc
      661 tgctcgacaa ctcgcttttt atcggccatc gtagggcccc ctcccaagtg cgcgcaccgg
      721 acagccagtc gatgcgatat ctcggttttc acgtctcgcc ctagaagcaa gctctcgagg
      781 gcaatagcgt atagaaggaa ggcttcctct cgcctagctt caacttgggc cggcccagcc
      841 cactgtatcg aggcgagaat tctttcctca agatcagttg gaacttcctc gccaagcatg
      901 tcagaaaccc ttgggaaccc attgcggcga gcgatctggt ccaacggtat ccgtcggata
      961 ggtccggcct gccggaagct cggcgtaacc ctgccatctt cgcagaccgc gagaacaaac
     1021 tcgcgctgcg gcatcgtatc cccgggaagg aaagcccagc cacccaatgc ttcacgcgag
     1081 gaaaagaagt tgatcgcatc aattgtcgtc tgcaacttgt gcctcgcagc cagaattgct
     1141 gcctcgccgt caaccgcgtt gaccgaaagc actccaatgg tcttgccctg taggagtttc
     1201 aggctgctct cgaggtatga cgccgcgtca tcgctattct tgacacccac gttccttact
     1261 gcgtcgagta cctggttttg taggtttgaa aacgcagcct cctcgagata ttgaaactgc
     1321 acttggccga cattcatcgg caagccgctg gatgcaagac cgtctacgat caaccgtacc
     1381 tcccaattct tcggctgctc aaacaatgcg ttcttcagcc cctgcatctc atcctcgacc
     1441 tgcttggcga gacttggatt gtcggtacga tctccagaac tcagcattcg cctaaaaaaa
     1501 tcatcgagaa tgccgcttac ggcccgcttg cttactggcc tcctctcgga tacagccatg
     1561 taaatagaag acaccatttc tgtgtacttg tcggcgtctt ccccgatcaa gatcacggta
     1621 tgatcgccgc acgtcattag caccgtggag tccttgaaga aaccctcgtc gaactggcca
     1681 ctgcaatcca gattttgaat aaattcggcg aaggagcgaa gtgttccgtt gaggtccata
     1741 caatccttcg aaaaacgccg agatccgttc aagcgaggct tgagtgggga tctgcgaaaa
     1801 tctccgttat ttcgtttccg ccttcgcggc ccgcttcttg cgctgcgtcg ggtgcacaaa
     1861 ccatgcaatg gcgatgctga acaggtacag cacgatcatg ggcgcggcga agatgcacat
     1921 gttcatgatg tcggtggtgg gcgtaatgat ggcggcgatg atgaagatta ccaggatgga
     1981 atagcggaag ttgcgccaca tccagcccgc cgtcagaatg cccatcagcg agaggaagaa
     2041 caccaggatc ggcatctcga agacgatgcc cagcccggcg atgatggtca ggaacaggtc
     2101 ggtgtattcg ccgacggtga tcatcggctt gaactgggcg ctccagccaa tcaggaaatc
     2161 cagcgccgcc ggatagacca tcttgtagcc aaagaagccg cccgccagga acaggcccac
     2221 ggtgctgaac atgaagggga acacgtagcg gcgttcgttg cggtaaaggc ccggagcgat
     2281 gaacgcccac acctgataga gcacgaacgg cgaggccacg aaaatccccg ccaggaacgc
     2341 gatcttcagg tacatgttga agggatcggt ggggttcagg tagaccagtt gcgtgtccat
     2401 cttgtgattg gccagggcgg tcacgatggg cttctgcatg atggtgaata tctgctcgtg
     2461 aaaccaccag cagacgcaga agccggccac tacgtaaaga aagctgtaga tgatccggcg
     2521 ccgcagttcc tccaggtgct ggaggaaact cattccggtc atttcctcgg aagcgcgttt
     2581 ctgtgccgtt tccacgatgg ggctaggcat cgggagttga cctggcgggt gtgggaggct
     2641 cgttcataga gggtgcagcc gggagttccg ccggtgcggg atcagtttcg agttgagaga
     2701 ttgcatcggc gctgccccgg ctgacgatcc ccaccggagg caggatggtc gcctcccacg
     2761 acttctcgga cggcaaagcc tctgccgcct cagtttgctt cttagcgcgc tcatccagct
     2821 caatattcat catctcggtt tcgagttggt tcttgaagtc gttgctggca cgtttgaact
     2881 cggcgaccca ttttcccatc tgccgggcca acccgggcaa tcgtttgggg ccgaccacca
     2941 ccagtgccag cagaaaaatg aagatcatct ctggcatgcc aaggttcatg ggatcatcat
     3001 aaatgcggag ggcctgtttt caccacaaag acaactctca gccgtcagct ctcagtgaat
     3061 ctcgctattc gcttttcgcc cttcgctttt tcgcttgagg atgaatccat ttcccttgtc
     3121 atcctgagcg cgggcgcgcc tcctttgcgc gccggagtcg aaggacccct atccctgcaa
     3181 aaaaccttcc tatgcgagat ccaagtgcca agtgcccaac tgccaagtgc taactgccaa
     3241 gtgccaagtg ccaactgcca cgtgctaact gcctggctcc tgccctgctc accaagccat
     3301 ccttccaacg tctgttacgg agacggggac caaaacggat ttcgcaccct tctctcccaa
     3361 ccagcatcca aaatagcggc tgatctggct ggctcggggt gggctaaagt gcatggtata
     3421 ctgcgtgaaa gtcttgctcc ggtgcgcata ccttgaagat tcatccccgg tcagccga
//
LOCUS       DASRKK010000595         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1323275, whole genome shotgun sequence.
ACCESSION   DASRKK010000595 DASRKK010000000
VERSION     DASRKK010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323275"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1734..2312
                     /locus_tag="VFC15_14115"
     CDS             1734..2312
                     /locus_tag="VFC15_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11338.1"
                     /translation="MRTCKHLKANGEFCGSPALRGRDYCYFHLTVLGRRLRMQKHIMK
                     GENHHLQLPTLEDGDSIQMALMQVMEALLRDQIDNKLAGLLLYGLQIASSNLQQGANF
                     AQGKSATVAGSYDNFERDYDLADTDCGLRVPQEEEELEAKAGKEIDLAGAVRTAPEPQ
                     RIQKDAGSQGFLVRLRPQNDETQLAVETMEVA"
     gene            complement(2318..2473)
                     /locus_tag="VFC15_14120"
     CDS             complement(2318..2473)
                     /locus_tag="VFC15_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11339.1"
                     /translation="MGIGAQILSDLNLKRIRLLTNHPCKVAGLEGFGTEIVEQVPVSA
                     AGKASVR"
     gene            complement(2516..>2867)
                     /locus_tag="VFC15_14125"
     CDS             complement(2516..>2867)
                     /locus_tag="VFC15_14125"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZM11340.1"
                     /translation="NASRGLAGTPGIGPGNFFLQPGAKSLQEIIGDIKEGLFVTEFLG
                     FGVNLVTGDFSRGASGLWIENGELTYPVEEITVAGNLKDMFFNISEIGNDLEFRGAIA
                     SPTLRIDGMTVAGE"
BASE COUNT          638 a          799 c          807 g          623 t
ORIGIN      
        1 gccgacggcg gcccactgat ctacaacggc tcggaagagg agcaacgctc cctcgattcg
       61 acctgccgca aactccaccg cttcctcaat caaccccagc gctgagcccc aaaacaccca
      121 cgaatccttc ggacgaggca atagtctagc gtcagcataa ctggaaggtg gtcggaccca
      181 acgctactgt ccggtcgccc ttgacgggtc agcaaagata tctcacctat gttcgaaatg
      241 attcgaacgt cctctgtacg gaaattcttc agcaaagatg gtcgaatgag aatctggtca
      301 aacatattcc acatgtactc aacgtgctct gaccgttcgt agtaataagt tcctggggga
      361 cctgaggagt gatcgcccaa atggctccac ataggattgt agaaaaaggg atacgacacg
      421 cctttaaccc gtctctctgc gcgttgcgcg actgtatggc tcatgacagc atggaaacct
      481 tcggtggcta caatcccctc ctcgaaagga ttaacattaa ggtctcccag aaatattgtt
      541 cgtctgtgac cgatactggt ctcgactgat gatattttcc tcgccaacct ggtacattca
      601 tgaacctgac tagcggtgct ttggtagagc ttgctggtta agtgcgctgc cgccaggatg
      661 atgtcgtccc gagctggcaa tgtcatctga actattgtga aatagtttcc ctcttctcta
      721 ggtcgcacga agtcgggatc aaaacttgta aacatgcgta tcctggcgca attgctgggc
      781 acaaccttgt agaagatttc tcctgaacca agtgcatcga ggataatgtt ggtaggaatt
      841 ttacattcag ctaagaacaa tatgtcaggt gagtaaactt tcgcgactcg agcaagcaaa
      901 ttctcgacag gcttgccgtt caggttccag aataagagct tcggcatcgt gagttaggaa
      961 acaatccaag agttcaatcc tgcattctat caatcagtac ttctttggca aaacattggg
     1021 tcgggtgacg ggtgaccggc caagctgcgg gccgccccgt tctagtccgg ttttggctag
     1081 ggcggggcaa ttgccaggac agaatcctgt gtcgtccccg gggggactcg actctctttt
     1141 tcagtctacc caccgttgaa acggtgggct atcatctttc gcgcccgagg cgcttcatga
     1201 agcagcgtta gcggataacg aacggcccga ttcgcgccgc tataggggtc cttcgactcg
     1261 gtcgccgcgg cgacctcgct caggatgaca ggtcaccaag agctgatggc tgacggctga
     1321 aagctgacgg ctcacaccat tcccgttccg ggagacggcg caaaatagga gcgccaattg
     1381 atactttcgt aacttgaccg cattgccgac ctgacccata ctctgcttgt agggcaggat
     1441 ccgccgcgcc cggcgctcct gccctgttgt gtctgtagtc ggtttggtaa cgtcacgtga
     1501 cgaccctcca actgccaaac cgcgtttttc cagggctctc ccaccctccc tcagcagccc
     1561 gtaagtcttt gcggctcaat attttgcctg taaatgccct gaatacaata ttttgccggg
     1621 attttcttgc ggcagctctt tgttttcaat attttgcggt gaaggttggg gagggggtag
     1681 cagcgaaagg cgaagagcga acagcgaaca gcgaacagcg aaaggcggac atcatgcgta
     1741 cttgtaaaca cctgaaagcg aacggagaat tttgcggttc accggcgttg cgcggccgcg
     1801 actattgtta tttccacctc accgttctgg gacggcgctt gcggatgcag aaacacatca
     1861 tgaagggcga aaaccaccac ctgcaactgc cgaccttgga agacggcgac tccatccaga
     1921 tggcgctgat gcaagtgatg gaggcgctgc tccgcgacca gatcgacaac aagcttgcgg
     1981 gactgttgct gtatggcttg cagatcgcca gctcgaatct gcagcagggc gccaattttg
     2041 cccagggcaa gagcgccacg gtggcgggaa gctacgacaa cttcgagcgg gactacgatc
     2101 tggccgatac ggattgcgga ctgagggttc ctcaggagga agaagagctc gaggcgaaag
     2161 cggggaagga aatcgatctg gccggtgctg tccggacagc gccggagccg cagcgcatcc
     2221 aaaaagatgc cggcagccag ggctttctcg tgcgattgcg tccgcaaaac gatgagacac
     2281 aacttgcggt ggagacgatg gaggtcgcgt gagggcttta tcgcaccgac gcttttcccg
     2341 ccgccgacac cggaacctgc tccacgattt cggtgccgaa gccttcgagg ccggcaacct
     2401 tgcagggatg gttggtgagc aggcgaatgc gcttgaggtt caagtccgac aggatttgcg
     2461 cgccgatgcc gatctacagc tgcgtcggtg tcgaaccttg cacttggacg gtgccttact
     2521 cccccgcgac ggtcatgccg tcaatgcgaa gcgtgggcga cgcgatcgcg ccgcggaatt
     2581 cgaggtcgtt gccgatctcc gagatgttga agaacatgtc cttcaagttg ccggcgacgg
     2641 tgatctcttc cacgggatag gtgagttcgc cgttctcgat ccacaagccg ctggccccgc
     2701 gcgagaagtc gccggtcacc aggttcacgc cgaagccgag aaactcggtg acgaacagac
     2761 cttccttgat gtcgccgatg atctcttgca ggctcttcgc tccgggctgg agaaagaagt
     2821 tgcccggccc gatgcccggc gttcccgcca gcccgcgcga agcgttg
//
LOCUS       DASRKK010000596         4557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1225933, whole genome shotgun sequence.
ACCESSION   DASRKK010000596 DASRKK010000000
VERSION     DASRKK010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225933"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..703
                     /locus_tag="VFC15_14130"
     CDS             302..703
                     /locus_tag="VFC15_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11341.1"
                     /translation="MNRKITPPIPEAIVQLQRQLDQFRSTQARRTKLPESLWRAAVEL
                     ARQHGIYPVAHPLRLDYMGLKKRLGGTPIVRRKASKPAFVELITPAATPPEECVIEFE
                     SLRGGKVRIQWKASAPPDWGSLLRAWREAEG"
     gene            700..1050
                     /gene="tnpB"
                     /locus_tag="VFC15_14135"
     CDS             700..1050
                     /gene="tnpB"
                     /locus_tag="VFC15_14135"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZM11342.1"
                     /translation="MIQIAPQIRILVAVEAIDGRKGIDAIAQLCREKLAADPFSGYLF
                     IFRTRRGTAIRVLQYDGQGFWLATKRLSKGRFRWWPTGTEAVRTLRAHQAQLLLAAGN
                     PDAEAPPAWRPLSA"
     gene            1137..1982
                     /locus_tag="VFC15_14140"
     CDS             1137..1982
                     /locus_tag="VFC15_14140"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HZM11343.1"
                     /translation="MVTADDITSIRQLIAENPGASRRRLSEKLCEAWQWKQANGALRA
                     MVCRGLLLMLHRAGEIQLPPVRYQTINPFVVRTTPAPMLIDTTPLAGTLKLLQPIELQ
                     QVRRTGDEPLFNSLMEQHHYLKYEQPVGEHAKYLAWAQGRPIACLAWSSAPRHLGSRD
                     RYIGWNAEARRRNIRFIAYNTRFLILPWVTVPHLASHLLGRMAAILSDDWQRMYSHPI
                     YFAETFIDPSRFRGTCYRAANWQSLGLTTGRGKNDQTNKPNRPIKEILGLPLTPRFRE
                     YLSQV"
     gene            1985..3505
                     /locus_tag="VFC15_14145"
     CDS             1985..3505
                     /locus_tag="VFC15_14145"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM11344.1"
                     /translation="MRRRVDVNLDELDQIIDRSTRAPLSEAEGQKLKAALHAMAERLL
                     RKRNTEKTSAVLPPDAVPAGKPDSSQSAPAGHGRNGAAAFTGANRVAIAHATLHAGDQ
                     CPECRRGKVYRQKEPATLVRFVGHAPLEATVFEMERLRCNACGEVFTAEEPDAAGAAK
                     YEETAVAMIALLKYGTGVPFKRLERLQGQLGMPLPATTQWELMEAAAKPFRPVLEELI
                     RQAAQGSVMHNDDTSMRILRLTREPDDKRTGTFTSGIVSIVGAWTIALFFTGWKHAGE
                     NIAEVLKQRASGLPAPIQMCDALSRNTPKGVETLLANCLAHGRRQVVEVAENFPEECR
                     YVLETLRDVYHHDAQAREQAMSPEQRLLFHQEHSGPLMKDLHEWMEAQLAEHKTEPNS
                     GLGKAISYLLNHWPKLTLFLRQAGAPIDNNIVERALKKAILNRKNALFYKTLNGAGVG
                     DLFMTLIHTCELNGANPFDYLTELLRHAEELKRSPSEWMPWNYRETLARLARPAVA"
     gene            3640..4224
                     /locus_tag="VFC15_14150"
     CDS             3640..4224
                     /locus_tag="VFC15_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM11345.1"
                     /translation="MFRAGLYARVSTNDQQTLPMQSCALREYAARRGWTVAMQVREVG
                     SGAVKREAREKLLEAARRREIDVVLVWRLDRWGRSVTDLLATLQELEHLGVGFVSLTE
                     ALDLTTPAGRAMAGLLAVFAAFEREILGERTRAGLAHARENGKRLGRPATAAVHGAEI
                     RKLHRAGVSKSEIARRVQIGRTSVRRILGDSSQK"
     gene            complement(4230..>4557)
                     /locus_tag="VFC15_14155"
     CDS             complement(4230..>4557)
                     /locus_tag="VFC15_14155"
                     /inference="COORDINATES: protein motif:HMM:NF017386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HZM11346.1"
                     /translation="YVIEDHLAALIETIELVAPEQELEFRAEFQAALTAAVDKRDRVG
                     QFLAHLEQQIDFARFEIDRLRQRKATFERALARLENYVIETIEQLGIDSKGKYRVLSI
                     SLRKEG"
BASE COUNT          943 a         1360 c         1440 g          814 t
ORIGIN      
        1 atccaagagg ctccgacagg cacgtcggca tacttagggg ttagcgaact ttgatccctt
       61 cccgcgtagt gggggatgtt gagcccggcg agcctgggcc aggcccgaga agccccacct
      121 cgcttcaacc ctcagcagga cttgccgcta ctgcgtgatc gtcagcgtcg ggtttgccgt
      181 ggccgtctga gccggaaagc tgctatcggt cactcggaat acggtgaggt ttcgaggagc
      241 gtgcgtgacg ccaggcggcc cgcgatgtta agttcagtca ggtgccaagg agcgtctgat
      301 agtgaaccgc aagatcacac caccgatccc tgaggcaatc gtgcaactcc agcgccagtt
      361 ggatcagttt cgcagcaccc aggcgcgacg gacaaagctg ccggagtcgc tatggagggc
      421 cgcggtcgaa ttggcccggc aacacggcat atatcccgtc gcgcatccat tgcggcttga
      481 ctacatgggg ctgaaaaagc ggctgggggg aactcccatc gtccggcgca aggcaagcaa
      541 gccggcattc gtcgaactga ttacgccggc tgcgacaccg ccagaggagt gcgtcatcga
      601 gtttgagtcc ctccggggcg gcaaggtgcg gatccagtgg aaggcatcgg cgccgcccga
      661 ttggggaagc ctgctgcgcg cctggcgaga agccgaagga tgattcagat cgcgccgcag
      721 atacggattc tggtggcggt ggaagccatc gatggaagaa aagggatcga cgccattgcc
      781 cagctatgcc gcgagaaact ggccgccgat ccattttccg gctacctatt tatcttccgc
      841 actcggcggg gcacagccat ccgagttttg cagtatgacg gccagggatt ttggctggcg
      901 acaaaacgct tgtccaaggg gcgtttccga tggtggccca caggcacgga agcggtgcga
      961 acgctgcgag cgcatcaggc gcaactgctg ctagcggccg gcaatcctga cgcggaagcc
     1021 ccgccggcgt ggcggccgtt gagcgcttga agaaatccgc aaaaagcact tgcgttccgc
     1081 tttcgactct ggcacactgg cggcagatgg cttccgaatg gagatatcga gggcgggtgg
     1141 ttacagccga cgatatcact tcgattcgcc agttgatcgc cgagaatccg ggagccagcc
     1201 gacgccgact ttccgagaaa ctgtgcgagg cctggcagtg gaaacaggcc aacggcgcgt
     1261 tgcgcgctat ggtttgccgc ggcttattgc tcatgctgca ccgggccggc gagatccaat
     1321 taccgcccgt ccgctaccaa actatcaacc ccttcgtcgt gcgaacaact cccgcaccga
     1381 tgctcatcga cacgacgccg ctcgccggca ccctcaagct gctccagccc atcgaactgc
     1441 aacaggtgcg gcgcacgggc gacgagccgc tgttcaacag cctgatggag cagcatcact
     1501 acttgaaata cgaacaaccg gtcggggaac acgccaagta tctggcatgg gcgcagggcc
     1561 gcccgatcgc gtgcctggcg tggagttcgg caccgcgcca tctgggcagc cgcgaccgct
     1621 atatcggctg gaacgcggaa gcgcgccggc gcaacatccg cttcattgcc tacaatactc
     1681 gttttcttat tctgccctgg gtcacggtgc cacacttggc gtcgcacctt ctcggccgca
     1741 tggcagcgat cctttccgac gattggcagc ggatgtacag ccatccgatt tacttcgcgg
     1801 aaaccttcat cgatcccagc cgttttcgcg gaacctgtta tcgtgcggcg aactggcagt
     1861 cacttgggct tactaccggg cgcggcaaga acgatcagac caataagccc aatcgcccga
     1921 tcaaggaaat cctcggcttg cccttgacgc cgcgtttccg cgagtatctc agccaggtat
     1981 aacgatgcga cggcgagtgg acgtgaacct ggatgaactg gaccagatca tcgatcgcag
     2041 cacgcgcgcg ccgctgagcg aagcggaagg ccagaagctc aaagccgcac tccatgccat
     2101 ggccgagaga ctgctgcgga aacggaatac ggagaaaacc agtgcggttt tgccgccgga
     2161 tgcggtgccc gcaggcaaac cagatagcag ccaatcggct ccggccggtc atgggcgcaa
     2221 tggtgcagcg gcgtttaccg gcgcaaatag ggttgccatc gcgcacgcca cgctccacgc
     2281 cggcgaccag tgcccggaat gccgccgggg aaaagtgtac cgccagaaag aaccggcaac
     2341 gctggtgagg ttcgtggggc atgcgccgct ggaagcaacc gtgttcgaga tggaacgcct
     2401 gcgctgcaac gcctgcgggg aggtcttcac cgccgaggaa ccggacgcgg cgggtgcggc
     2461 caagtacgag gagacagccg tggcgatgat cgccctgttg aagtacggta ccggagtgcc
     2521 attcaagcgg ctggaaagac tgcaaggcca actgggaatg cctttgccgg cgaccacaca
     2581 gtgggaactg atggaggccg ccgcaaaacc gttccgcccc gtgctggaag agttgatccg
     2641 gcaggcggcg cagggcagcg tgatgcacaa cgacgacacc agcatgcgga ttctgcgtct
     2701 gacgcgcgag ccggacgaca aacgaacggg taccttcacc agcggcatcg tatcgatcgt
     2761 gggtgcctgg accatcgcgc tgttctttac cgggtggaag catgcgggcg agaatatcgc
     2821 cgaagtcttg aaacagcgcg ccagcggact gccggcgcca atccaaatgt gcgatgcgct
     2881 gtcgcgcaac acgccgaagg gcgtcgaaac cctgctggcg aactgcctcg cgcacggaag
     2941 gcgacaggtg gtggaggtgg cggagaattt tcctgaagaa tgccgttatg tgttggagac
     3001 gctgcgcgac gtttatcatc acgatgcgca ggcgcgagaa caggcaatgt cgccggaaca
     3061 acgcctgctc ttccatcagg agcacagcgg accgttgatg aaagaccttc atgagtggat
     3121 ggaggcgcag ttggcggagc ataagacgga accaaattcc ggactgggca aagcgatctc
     3181 ctatttattg aatcactggc cgaaactgac gcttttcctg cggcaagcag gcgcaccaat
     3241 cgacaacaac atcgtcgagc gcgccctgaa gaaagccatc ctgaaccgca agaacgccct
     3301 cttctataag acgctgaacg gcgccggagt cggcgatctg ttcatgactc tgatccatac
     3361 ctgcgaactg aacggagcca atccgttcga ctatctcacc gagctgctgc ggcacgccga
     3421 ggagttgaag cgcagcccat cggagtggat gccttggaat taccgcgaga cgctggcgcg
     3481 gctggcaagg cccgccgtcg cgtaatatca tgattccttc ctggccgaaa aggatcgact
     3541 gcggccatgg ttaggtgacg ctctgaaatg gccgccaaac gggcctctcc aaaggcaaaa
     3601 tcgcataccg ttttcggcca ggcacggaag cccgcgaaaa tgtttcgcgc cggcctgtac
     3661 gctcgcgttt ctacgaacga tcagcagaca cttccgatgc agagttgcgc cctgcgggag
     3721 tatgccgctc ggcgcggctg gacagtcgcc atgcaagtcc gtgaagttgg ctccggggcg
     3781 gtgaagcgag aggcgcgcga gaaactgctg gaggccgcac gccgccggga gatcgatgta
     3841 gtactggtat ggcgactgga ccgctggggc cggtcggtaa cggatctgct ggcgacactc
     3901 caggagttgg agcatctcgg tgtgggcttc gtgtcgctga ccgaggcgct ggacctgact
     3961 acacccgcgg gtcgagcgat ggctggcctg ctggccgtct tcgccgcctt cgaacgggag
     4021 atcctgggtg agcgaactcg ggctggcttg gcccatgctc gggaaaacgg gaaacggctg
     4081 ggtcggccgg ccaccgcggc cgttcacggt gcggaaatcc ggaaacttca tcgtgctggt
     4141 gttagcaagt cggagatcgc tcgtcgagtc cagatcgggc gcacctcagt ccgccgaatc
     4201 ctgggcgatt cctcccagaa ataggtcggc tagccttctt tacgcaggct tatcgaaagg
     4261 acacgatact tccccttgct atcgatcccg agttgctcga tggtctcgat cacgtagttc
     4321 tccagacgtg ccagagcacg ttcgaaggtc gccttgcgtt gcctcagtcg gtcgatctcg
     4381 aatctggcaa agtcgatctg ttgctccaga tgggccagga actggcccac gcggtcgcgt
     4441 ttgtcgacgg ctgcggtgag ggcggcctgg aactccgccc gaaactccaa ttcctgctcc
     4501 ggggcaacca gttctatggt ttcaatcagg gccgcgaggt gatcctcgat cacatac
//
LOCUS       DASRKK010000597        18300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_53417, whole genome shotgun sequence.
ACCESSION   DASRKK010000597 DASRKK010000000
VERSION     DASRKK010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18300
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53417"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2008)
                     /gene="dnaX"
                     /locus_tag="VFC15_14160"
     CDS             complement(134..2008)
                     /gene="dnaX"
                     /locus_tag="VFC15_14160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZM11347.1"
                     /translation="MSYQVLARKYRPQKFSDVIGQEHVTRTLKNAIEQQRIAHGYIFS
                     GHRGIGKTTIARILAMALNCRSKDHPVPEPCGVCDSCLEIRAGNAVDVIEIDAATNRG
                     IDEIRELRDAARYRPARDRYKIWILDEAHQITDAAFNALLKTLEEPPSHVIFMMATTQ
                     PEDIPQTIRSRCQHFSFHAVSFDDIVGQLRDIARQEGITADDNALAMLAEAGDGSMRD
                     ALSIMDQAIASCGDTLSADVVRGLVGTVGSDTLEDIMDAVACDSSEDVLRTIDRLLVE
                     GQSAQHFARQMVRFLRNTVVAKVAGSDTPVLQISSDERARVARVAERFSEEDLTRFLQ
                     IMLRTHSDLGYKQEQRFHLELGLLKLVHAQRLLPLEQLLSGEAAKPRGTVSRSGSSLP
                     GNASTAVARSAPAATRTSAPEASSSRGAATSPFKGPSPFESDRSRKSEPKAETPAITS
                     ALVGTPSSVAGSTPVASTAIESSVAVAVAIASEDPPADATLEVTPDVARNAVLGALED
                     AGQQMLAHNLESGEWSVRSHEVSVKVAMSQVMVDVALGEGPKRIVQEALLKATGKPLK
                     FKMVSGGSQFTPKAAPPSARPANGAGARSRAMSDPIVQRMQEKFGAEIRSVIDHKERG
                     "
     gene            2096..2548
                     /locus_tag="VFC15_14165"
     CDS             2096..2548
                     /locus_tag="VFC15_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM11348.1"
                     /translation="MQALQSLRTLLESLPARLEALPAEAVDFRPPESWSVKEELGHLL
                     DSAANNHQRVVRGQLEDSPAMRGYDGEKWVSLHAYQVRDWHELIGTWEALNRQLLATA
                     EAIPDSAWSRTLTIGESAPLTLQFVLEDYIHHMRDHLRHMKVPIDDLE"
     gene            complement(2562..5138)
                     /locus_tag="VFC15_14170"
     CDS             complement(2562..5138)
                     /locus_tag="VFC15_14170"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11349.1"
                     /translation="MTKANLTSTQLLNPFPGLRPFHENEEHLFFGRESQIDTMVDELA
                     ATRFLAVVGTSGSGKSSLVNCGLKPALSRGLMSSAGTSWRMAQFRPGGDPIKAMAHAL
                     AQPGGLFSQAFEGLSVEEIIEATLRMSKLGLVDIYEQAHLEERPNLLVIVDQFEELFR
                     YRNIQPSSEESSGAEDRSVAFVNLLLEAASSNRPIYVVLTMRSDFLGGCSQFPGLPEA
                     INRGQYLVPRMSRDERRSAIAGPVAVAGGEISPVLLTRLVNDVGDNPDQLSILQHALN
                     RTWAQWQRVGGIGAMDLPHYEAIGTMSRALDLHAGSAYAELRDDRQRIICQKVFQALT
                     DKGPYLRGVRRPTSAETLCLINDATLDELKLVLDVFRKPSRSFVMPPMPEPIAPETVI
                     DISHESLMRIWNRLKGWVDEEAESASQYERLVQNMSLHAKGGAGLMTDPELSLMLEWQ
                     QKWQPNVAWAERYRLGFDSAIAFLDDSREARDAALLADEERKRRELRRTRQIAAVLGT
                     AFVLAVAFGIYALFERSRATEERVARRQTEALSHAKQKLLEEQERARKESEELNQKLT
                     VALDTAQKATVAAEAADARAEHERDLATRTAQMFITAIKAQAAAQLAAFEQLEKEEIL
                     QQDRDARKGAAALDQDKQELDAVTRQAKQLSLDAARKSAEAAAIVANAHILSPSQISG
                     SDLFDVEHGQSQVTGYSGGANTRNPNDMFSGSGGSPDRATVFADGQQVGTEHWIEWKT
                     RKDVTLNSAGLFAAHDAIRLRRSFSSFKLYVKKQGQWTLVTEYNPALMYGGSCGSQPC
                     FPPPAIKYAPGTVLAACLNVPPATGQEFRAVFVQSVSSVERFSGPRVLQLDGYNTSNC
                     SK"
     gene            complement(5135..5815)
                     /locus_tag="VFC15_14175"
     CDS             complement(5135..5815)
                     /locus_tag="VFC15_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11350.1"
                     /translation="MVLENELAIQYSRQFGLKRIIWVPSGTKSESSRQQQFIDQLLRD
                     PVAQFGADLITGDLEDLKAAVHAALKKLETPAPPPPPKSSAEQDTKVIYLICDERDRK
                     ATIPLRKLLKSRGFESYIPTFDGDAATVRQSNQNLLNSCDAAVIFYGAGDESWKRSVD
                     AEIRKVAGYRTSKTPLAPFTYLAEPATDDKTDLVEMEEPRLINCLYGFSEAAVADFVS
                     SVSGTVKP"
     gene            6544..7158
                     /locus_tag="VFC15_14180"
     CDS             6544..7158
                     /locus_tag="VFC15_14180"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11351.1"
                     /translation="MVTPNPVNLYRAPANDNGWFLSTPDSSSSITIQFGSSQFVNNVW
                     HCSGSCITGFDFYWESVDTWNEVTFKDQNGNTTSFYGSDLGMCGISTSGDGSCFPFTD
                     PNHNNPGNPDLIPALIDFSPGGSATSWQSVTITSSNSAGNYYPAFELDNLEFTCNDTS
                     SCDPNIYPSLTPFKSPPTPEPSSMLLLGTSIAGISMLLRRRMRA"
     gene            complement(7193..7963)
                     /locus_tag="VFC15_14185"
     CDS             complement(7193..7963)
                     /locus_tag="VFC15_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZM11352.1"
                     /translation="MFQRAIVRKPGDNFANGLTSAGLGMPDLSAALSQHEAYCRALES
                     CGLQLIRLPADLQHPDSTFVEDTAILTSRSAILTRPGAASRLGEVTGVRETIDRFYSS
                     IHQIAAPGTLDGGDICEAGSHFFIGISHRTNQEGARQLSQWLAADGFTSSMIDIRDQR
                     SILHLKSGIAYLDKNDLVVMEELASREEFSGYNRIRPLPEESYACNCVLVNDRVLVPA
                     GFPRLQEVLSLLGYKPLPLDMSEFRKMDGGLSCLSLRF"
     gene            complement(8051..9043)
                     /locus_tag="VFC15_14190"
     CDS             complement(8051..9043)
                     /locus_tag="VFC15_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZM11353.1"
                     /translation="MQNGQNVAPEVAQTPDLLALIEDATNRIYGLVQQTPVARLPRDP
                     RIPATTRAFAKMEQLQLTGSFKLRGAANKVMGLSPQAALVGVVASSTGNHGLAVAAAA
                     RHRGIDAEVYLSAQVPEAKWKKIASYGARIRIVGQNPLEAEVAARAAALESGRTYVSP
                     YNDWEVVAGQGTIGAELVHQVESLDAVFIATGGGGLISGVGAYLRERCRHTEIVGCWP
                     QNSRVLYECLQAGRVIDYPESYTLSESTAGGVETGSITLELARAAMHRGVLVSEDAIL
                     NAMKWAREQNWTIEGAAGVALAAYLQEAGRYEGKSVVVLFCGGNLSPEVQSRMQ"
     gene            complement(9143..9862)
                     /locus_tag="VFC15_14195"
     CDS             complement(9143..9862)
                     /locus_tag="VFC15_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZM11354.1"
                     /translation="MKVLAIDVGGTNVKLLASGHEARRKFASGPTLTAKQMVDEVRKV
                     AADWKYDVVSIGYPGPVLDGRLLAEPHNLGKGWVGFNFEKAFSCPVKIVNDAAMQALG
                     SYKRGRMLFLGLGTGLGSAMIIDGELLPMELAHLPYRKGTYEDYVGLRGMKRFGKKKW
                     RSYVADVVEKLSAALEPDDVVLGGGNVKFLKTMPAKCRAGDNANAFKGGFRLWEDSAM
                     PPAATKPHAVSAGGSRPVARS"
     gene            complement(9988..11049)
                     /locus_tag="VFC15_14200"
     CDS             complement(9988..11049)
                     /locus_tag="VFC15_14200"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZM11355.1"
                     /translation="MFYSTDDLRIKWTKVVLPPVFLDEELPTTEKASATIHEAREQIV
                     KILRGDDQRLLVVVGPCSIHDTKAAREYAARLKSAIDEHGKELHIVMRVYFEKPRTTI
                     GWKGLINDPYLDQSYKINDGLRQARHLLLDLAEMGVPAGTEFLDMITPQYVAGLVSWG
                     AIGARTTESQVHRQLVSGLSCPVGFKNATSGDVQIAIEAVLSAAHPHTFLGHTKHGQS
                     AIFATTGNPDCHVILRGGRSSVNYTAEAVASTCGKLEKAGLKPNVMIDCSHANSNKDH
                     TRQGAVCRHVASQIASGDRRIIGVMLESNLVAGSQSLVPGKSLTHGQSITDACMGWDE
                     TVGLLRELADSVRTGRPRS"
     gene            complement(11177..11488)
                     /locus_tag="VFC15_14205"
     CDS             complement(11177..11488)
                     /locus_tag="VFC15_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11356.1"
                     /translation="MFARNAYFRLKSLTLAAEFARTLEDEILPLLRKQAGFKGEITLS
                     NPGSLEQIAISLWDNKHDADAYNTGAYSQVLKALSKVIDGTPRIRTYEAVTLNLDRGK
                     E"
     gene            complement(11555..12979)
                     /locus_tag="VFC15_14210"
     CDS             complement(11555..12979)
                     /locus_tag="VFC15_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11357.1"
                     /translation="MIRTSRNLVVLVFAIIGFSILASADLKIKTRTTVMGHSTESTVY
                     IKGPRERTEMSFGGHAGAVTIMQCDEKRMITISGNQCSVIPTGGGETSCPTMPNMRAM
                     GREGPGNEPAAPRTGGVVTITRNSTDTGERQDIFGYKARHIKTSMIMESTPDACNQSH
                     MRMETDGWYADLSAGFSCADESYRAMACGGMGGRASCNDRIVMKGGGSAAMGYPMKQT
                     TTIMSEHGNFTTTTEVVELASTTLDAPLFDAPPGCKVTDMSAMMSGTPPNTPPESSHA
                     PGVAAPAPKAPAAPAPTPVATVAPKSAGVVRIGVVKIKDTSGEGLPTDNLRLDLMDEI
                     THHQMEAVPLDAEAPHQDVESEASSKQCDYILYTTASQVKAPGSGLPPASLPKGVTLD
                     AAKFQALTDVTLYKVGKPQPEMKDLLVAADADQFGVNAVMATFTQESANVAQQVDEDA
                     HPKPASKTTKAAVKKSVAAAPKPK"
     gene            complement(13124..13197)
                     /locus_tag="VFC15_14215"
     tRNA            complement(13124..13197)
                     /locus_tag="VFC15_14215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13163..13165),aa:Gln,seq:ctg)
     gene            13396..14004
                     /locus_tag="VFC15_14220"
     CDS             13396..14004
                     /locus_tag="VFC15_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZM11358.1"
                     /translation="MTSILSTAAFPADATDVARQPLVADQCALIVVDIQEKLLPPIFN
                     KDELVKNSQLLVRAANVLAMPVVVTTQYAQGLGPVVPDISSLVNDLRSIDKLEFGCFG
                     SNEFRSALKSLPGNRNTVLLCGMEAHICVMQTALGALNDGYLVHVASDAIGSRVRWNW
                     DIGIDRMRAAGAVISTTEMMIYELLRSSGTPQFKQMLPYLKG"
     gene            14057..15406
                     /locus_tag="VFC15_14225"
     CDS             14057..15406
                     /locus_tag="VFC15_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM11359.1"
                     /translation="MISLARKLRLADFFSLGFGTMIGVGWLVVMDDWLGRGGPLGAML
                     GFAVGGALLLPIGYVYGQLVRAMPDAAAEIAYTSAVFPRSVGFATGWMMLLAYCIVCP
                     WEAVAIGRIASYIFPAMNSIELYRVAGKPVFLPHLVIGLALTVLLTAMNYRGIRMSAS
                     FQNVTTFGAIALVLSFIAAGLRHGSVQNFQPLFSTSAPISILLVLQIVPYFMTGFESI
                     GKSSEEASSEFQSRRFMLAIMAAMLVGIGFYVCVIAATAYVAPWQSLVHERFATAVAF
                     ERAVGSRWVVSVITCAAVLSLLEVFNGNFVAATRLLFALGRRGLADSRLGYVHPVNRT
                     PAIAVIGIGMFTAACMFAGEAILVPIAEVGSLASALGWMAACASYLRMRPSSFGRAAA
                     ASGIVVTFLILAMKILPTVPGHFSRYEWAALALWILLGLLLRRRAGHSEAEGIRERI"
     gene            complement(15403..15930)
                     /locus_tag="VFC15_14230"
     CDS             complement(15403..15930)
                     /locus_tag="VFC15_14230"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZM11360.1"
                     /translation="MFARSATLPDAIAIDQLIQVHVGDGTLLGRTLAEICENIRDFVV
                     VEHDGEVVGCGALHLYGMHLAEVRSITIGPKAKGHGAGRLLIDSLLAEAARQSVTCVC
                     LFTRIPDFFGKMGFTVVDKELLPDKMYKDCLRCPRLHACDEIAMYMGELPEKTVLSSS
                     PHAARYYTQLVALES"
     gene            complement(15917..17305)
                     /gene="argH"
                     /locus_tag="VFC15_14235"
     CDS             complement(15917..17305)
                     /gene="argH"
                     /locus_tag="VFC15_14235"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZM11361.1"
                     /translation="MWSGRFSQDQNAGFEQWQRSFPFDRILIPYEVAASKAHAAALAK
                     AGVLDSGELSLTLTALDQIAAEGIPSVDDPAIEDVHHYVEKRLVEVAGEVGLKLHTGR
                     SRNEQIATDLRLYVRAQVDAISELLVELAQQFVSQAESAGDAAMPSYTHLQRAEPVLV
                     AHWLLAYVEMFLRDLGRLADCRKRLNLCPLGSGAVAGTILPLDRAGIAKALEFDAPTA
                     NSIDATSDRDFAIEFVQALSSVALHLSRWAEESIIFATTEFGFVKLPEEFSTGSSAMP
                     QKKNPDALELIRGNAAKVFADAMTLFITLKGLPLAYNKDMQETQHPVFAATQQVASML
                     RVATGFMASVTFDFERMREAASGGFMNALAGAAFLVRNGVPFRHAHEMIGRAVQLCVE
                     KGCELEPLSTEDYASCGIQAGPQFYEALQLQQVLAIHDVPGGTASAQVRAALKKTKET
                     LSMFAGAAHVCA"
     gene            complement(17308..>18300)
                     /locus_tag="VFC15_14240"
     CDS             complement(17308..>18300)
                     /locus_tag="VFC15_14240"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZM11362.1"
                     /translation="PAIQAGAVYEHKYLLGTSLARPIIAKYQVECALKEGATAVSHGC
                     TGKGNDQVRFEHAYQALAPELKVIAPWREWTLKSREDCLEYAESHGIQVAQSREKIHS
                     RDRNLMHVSHEGGELEDPNNAPLPTTWTWTKSPQEAPDKTEKVSIEFKHGLPIAVNGV
                     VLDPVAIVAVLNEIGARNAIGRIDLVENRFVGMKSRGCYETPGGTLLLTAHRELEALC
                     LDRDLAHFKQHIGLKYAELVYFGLWFTPLRESLQAFVKSSQADINGTVTLSLYKGNID
                     IAGRKSETSMYRPDVASFTMGTSYDQKDAEGFIRILGLPARSRALIHQKTEVAK"
BASE COUNT         3568 a         5402 c         5358 g         3972 t
ORIGIN      
        1 ggcctcgaca gttatttgct gcattttttc ctgcagcttt tcggcctgct ctcgcgcctg
       61 cgccagcata tcctgcaaat tgaatccacc catgaatgct ccaactttcg cgggacgtaa
      121 tcgtgccgtg aggctagccg cgttccttat gatcgatcac agaacgaatt tcggcgccaa
      181 acttttcctg catgcgttgc acaattggat cggacatggc gcgactgcgc gcgccagccc
      241 cgttcgcagg acgagccgac ggcggtgctg ccttgggcgt gaactgcgac cccccgctca
      301 ccatcttgaa cttcagtggc ttgcccgtgg ctttgagaag ggcttcctgc acgatgcgtt
      361 tgggtccttc gccgagcgcc acatcaacca tgacctgcga catggcgacc ttcacggaga
      421 cttcgtggct tcgcacgctc cactcgcccg actcgaggtt gtgcgccagc atttgttggc
      481 cagcatcttc cagagcacct agaacagcat ttcgcgccac gtccggagtc acctcaagtg
      541 tagcgtccgc tggaggatct tcgctcgcga tcgcaaccgc aacggctacg gacgattcaa
      601 ttgcggtgct ggcgaccggt gtgcttcccg cgacactact cggtgtgccg accagcgcgc
      661 tggtgatcgc cggagtctcc gccttgggct cgctcttgcg acttcgatca gattcgaacg
      721 gcgatggacc cttgaatgga gatgttgcag ctccgcgcga cgaggaagcc tcaggcgccg
      781 atgttctcgt tgccgcagga gcactgcgag ccaccgccgt actggcgttt ccgggcagtg
      841 agctgccact acgcgagacc gtgccgcggg gcttcgccgc ttccccgctc agcaattgtt
      901 cgagaggaag cagacgttgc gcgtgtacga gtttcaacaa tcccagctcg aggtggaagc
      961 gctgctcctg cttatatccc aggtcagaat gcgttcgcag catgatctgc aggaagcgcg
     1021 tgaggtcttc ttcgctgaag cgctcggcca cgcgcgccac tcgggcgcgt tcgtctgacg
     1081 aaatctgcag cacgggggtg tcgctgcctg cgacttttgc caccacggtg ttgcgcagaa
     1141 agcgcaccat ctgccgcgcg aagtgctgcg cactttggcc ctcgaccagc aggcgatcga
     1201 tggttcgcag cacatcttcg ctggagtcgc acgcgaccgc atccattatg tcttcgagag
     1261 tgtcggagcc aaccgtgccc accagtccgc gcaccacgtc ggcactgagc gtgtcgccgc
     1321 aactggcaat ggcctggtcc atgatgctga gcgcatcgcg catcgaaccg tcgcccgcct
     1381 cggccagcat ggcgagcgcg ttgtcgtcgg cggtgatgcc ttcttgtctg gcgatgtcgc
     1441 gcagttgtcc gacgatgtcg tcaaagctga cggcgtgaaa actgaaatgc tggcagcgcg
     1501 agcgaatggt ctgcggaata tcttccggct gcgtggtggc catcatgaag atcacgtgcg
     1561 atggcggttc ttccaaagtc ttcagcagcg cgttgaaggc cgcgtcggta atctggtggg
     1621 cttcatcgag aatccagatc ttgtagcggt cgcgcgccgg ccggtagcgc gcagcatcgc
     1681 gcaattcccg gatttcatcg atgccacggt tggtggcggc gtcgatctcg atcacgtcaa
     1741 ccgcattccc cgcgcggatt tcgaggcagg agtcgcatac accgcagggc tcagggacgg
     1801 gatggtcctt ggagcggcag ttcaacgcca tggccaggat gcgcgcgatg gtggttttgc
     1861 cgatgccgcg atgtccgctg aagatgtagc catgcgcgat gcgctgctgc tcgatggcgt
     1921 tcttcagagt gcgggtgacg tgctcctgcc cgatgacgtc ggagaacttt tggggccgat
     1981 atttgcgcgc tagaacctgg taactcatga aaggaagttc gattatacgg tctgaggctg
     2041 tggaacggga gagcgccgga ttagggcata atattcgcaa atgggggatg aggaaatgca
     2101 agcattgcag tcgctacgaa cgctgttgga gtctctgccc gctcgactgg aagcgctacc
     2161 tgccgaagcc gttgatttcc gaccgcctga gtcgtggtcg gtaaaggaag agctggggca
     2221 cttgctcgat tccgccgcca acaaccacca aagggttgtg cgcggacagt tggaagacag
     2281 tcccgcgatg cgtggctacg acggagaaaa gtgggtttct ctgcacgcct atcaggtgcg
     2341 agattggcac gaattgatag gaacctggga agcgctcaat cggcagttgc tggccacggc
     2401 ggaagcgata cccgactcgg catggtcgcg caccttaacg atcggcgaat ccgctccgct
     2461 tacgcttcag tttgttttgg aagattacat ccatcacatg cgggaccact tacgtcacat
     2521 gaaagtcccg attgacgatt tggagtgacg ccgttcccat ctcacttgga gcagttggag
     2581 gtattgtagc cgtcaagttg gagcactcgt gggcctgaga accgttcgac cgaactaaca
     2641 gactgcacga acacggcacg aaattcctgg ccggtcgcgg gcgggacatt gaggcacgcg
     2701 gccaggacgg tgccgggagc gtacttgatg gcgggtggcg gaaaacaagg ctgagaaccg
     2761 cagctaccgc catacatcag ggccgggtta tactcagtta ccagagtcca ttgcccctgt
     2821 ttcttaacgt acaacttgaa gctgctaaac gaccggcgta ggcgaatcgc atcatgagcg
     2881 gcaaatagcc cggcgctgtt gagcgtcaca tctttcctag ttttccattc aatccagtgc
     2941 tccgtgccga cctgctggcc gtcggcaaaa acggtagctc gatcgggcga tccgccggag
     3001 ccactgaaca tgtcattggg attcctggta ttggcaccac cgctgtagcc ggtcacctgc
     3061 gattgtccgt gttcgacgtc aaataaatcg gagccgctaa tctggcttgg gctgaggatg
     3121 tgggcgttgg cgacgatggc ggcggcctcg gcggacttgc gtgcggcatc caagctcagt
     3181 tgcttggctt gcctggtcac ggcatcgagc tcttgtttat cctggtcgag cgccgctgcc
     3241 cctttcctgg catcacggtc ctgctgcagg atttcttcct tttcaagctg ttcgaaggca
     3301 gcgagttgtg ccgccgcctg ggctttgatg gcggtgatga acatctgcgc ggtccgagtg
     3361 gcgaggtccc tctcgtgttc cgcccgcgca tccgccgcct cggccgctac cgtcgctttc
     3421 tgggcagtgt ctaatgccac agtaagcttc tggttgagtt cttccgactc tttgcgggcc
     3481 cgctcctgtt cctccagcag tttttgttta gcgtggctca aggcctcggt ttgccggcgc
     3541 gcgacccgct cctcagtcgc ccgtgaccgc tcaaacagtg cgtagatccc gaaggcaaca
     3601 gcgagcacga atgccgtgcc cagcactgcg gctatctggc gggtgcgacg gagctcgcgc
     3661 cgcttccgtt cctcatcagc aagtagcgcc gcatcgcgag cttcgcgact gtcatccaga
     3721 aacgcgatgg cggagtcgaa acccagccga taccgttctg cccacgcgac attgggctgc
     3781 catttctgct gccactccag cattagcgaa agctctgggt cggtcatcag gccggcgccg
     3841 cccttggcgt gcagcgacat gttctgcacc agacgctcgt actgagatgc ggattctgcc
     3901 tcctcgtcca cccagccctt gaggcggttc cagatacgca tcaggctttc atgcgagatg
     3961 tcgatgacgg tctcgggcgc gatgggctcc ggcatcgggg gcattacaaa cgatcggctc
     4021 ggcttacgga atacgtcgag caccagcttc agttcgtcga gggttgcgtc gttaatcaga
     4081 cagagggttt ccgcactggt ggggcggcga actccacgaa gatagggacc cttgtcggtg
     4141 agcgcctgga acaccttttg gcagataatt ctctgccgat cgtcacgcaa ttccgcgtag
     4201 gcgctccctg cgtggagatc gagtgcgcgg ctcatggtgc caatggcttc atagtgcgga
     4261 agatccatgg cgccaatacc gcctactcgc tgccactggg cccaggtgcg gttcagagcg
     4321 tgttgcagaa tggaaagctg atcggggttg tcgccaacat cattgacgag ccgggtgaga
     4381 agcaccgggc tgatttcgcc tcccgcaacc gcaaccggtc cggcaatggc agaccttctc
     4441 tcgtctcgtg acatgcgggg gaccaggtat tgcccgcgat tgatggcttc cggcaggccg
     4501 gggaattgcg agcagcctcc gaggaagtct gaccgcatag tcagtacgac gtagatgggg
     4561 cgattggaac tggccgcttc cagaagaaga ttgacgaatg caaccgaccg gtcctcagcg
     4621 ccggacgatt cttcagacga gggttggata ttccgatagc ggaagagctc ttcaaactgg
     4681 tccacgatga ccagaaggtt gggtctctcc tcgaggtgag cctgctcata gatgtcaact
     4741 aaaccgagtt tgctcatgcg cagcgtggct tcgatgatct cctcaacgga cagaccttcg
     4801 aaagcctgac tgaacagtcc accgggctgg gccagggcgt gggccattgc cttgatggga
     4861 tcgccgccgg gacgaaattg cgccatccgc cacgacgtgc ccgccgagga cattagcccg
     4921 cggctcagcg cgggtttcaa tccacagttg accagcgaag acttgccgct tccggatgta
     4981 ccgacgaccg ccagaaaacg cgtggccgcc aactcgtcga ccatggtgtc gatctggctt
     5041 tcacgtccga agaaaaggtg ctcttcgttt tcgtgaaacg gtcgtagtcc gggaaacggg
     5101 ttcaatagct gggtactggt caggttggcc ttggtcacgg cttcaccgtg ccagaaaccg
     5161 agctaacaaa atcggcgacc gccgcctccg agaacccata gagacagttg atgagcctgg
     5221 gttcttccat ttcgacgaga tcagttttat cgtcagtcgc cggctcagcc aaataggtaa
     5281 atggcgcgag cggggttttc gaagtgcgat agccggcaac cttgcgtatt tcggcatcga
     5341 ccgaacgctt ccatgactcg tcgccagccc cgtagaagat gacggctgca tcgcaggaat
     5401 tcagcaggtt ctgattcgat tgccgaacgg tagccgcgtc gccgtcgaac gttggaatgt
     5461 aggactcgaa tccccgggac ttcagtagtt tgcggagcgg aattgtcgct ttgcggtcgc
     5521 gctcgtcaca aatcaaatag atgacctttg tatcctgttc tgccgacgac ttgggcggcg
     5581 ggggaggcgc gggggtttcg agttttttca gagcggcatg gaccgcggcc ttaaggtctt
     5641 cgagatcgcc ggtgatgagg tcggccccga actgggccac cgggtcgcgt aagagctggt
     5701 cgatgaactg ctgctgccgg gaagattcgg acttggtccc gctggggacc cagatgatac
     5761 gtttgagtcc gaattgcctg ctgtactgga tggcgagttc gttctcgaga accatgaccg
     5821 acttttcact gggaccgtcg ggtacaacgc catagttgtt tccgatgatg tgtacagaca
     5881 atctggagcg ggcaagcgtt gaagccagtt cgccgacgaa agtcgattct tcggtggaga
     5941 actgcagttc cgcgagaacc ggataaccgt gaagtcgaag atccgcttga acagcttcgc
     6001 ggtcctcgcg ccggtcgatg ctgcaatccg ccagataaac ggccggcttg gtctggaccg
     6061 ccgatgccgt agcagcctcc gagtcggcag cgaactgggc gacccgaatt ggggtaaatc
     6121 gtgatccggc gactcggttt cagtctcgtt ctgttgggaa tgctggtagt tggtacttcc
     6181 aaactctcag cccagtcgtg cagtggaacg acgacgtttg gcagcttcac gtctgcaaat
     6241 ggactactct cggccaactg gacctgtgac accagtgctg cagtcgcgat accatgcccc
     6301 gtttcctcca acaaccaatt tggcagtggc aattgcggct acgtggatgg tactaattct
     6361 gcgtatctta acggttgcga tactggggtt tctccgtgta gtgcccataa cttctggcac
     6421 acctacaacg ccactacggc tactcctcag aatagcctca acggaatgta catctgtggt
     6481 acaggtacta gttgtgtcac gtcgcccttt cagttcacgc ttacgggcaa cgcgacgctt
     6541 aatatggtca cacccaatcc ggtgaacctg tatcgagctc cagccaacga caatggatgg
     6601 ttcttgtcca cgcccgacag ctcgtcatcc atcacgatac agtttggtag ctcacagttc
     6661 gtaaacaacg tgtggcattg tagcggaagt tgcataaccg gcttcgactt ctactgggag
     6721 agcgtcgata cgtggaacga ggtcaccttt aaggaccaga acggaaatac gacttctttc
     6781 tacggctcgg accttggcat gtgcggcatt tcgacgagtg gtgatgggtc ctgttttcca
     6841 ttcaccgatc ccaatcacaa taatcccggg aaccccgatc tgatccctgc gttgattgac
     6901 tttagccctg gaggctccgc cacctcctgg cagagtgtaa ccatcacatc cagcaacagc
     6961 gcgggaaact actacccggc atttgagttg gataacctgg aattcacgtg taacgatact
     7021 tcaagctgcg acccgaatat ttacccctcg cttaccccat tcaagtcccc gccaacgccc
     7081 gagccatcga gcatgctgct gctaggaaca tcgattgccg gaatttctat gctactgagg
     7141 cgtaggatgc gagcgtaggc tgaggcggtt cgcatcggcg tcgcgaatca actcaaaacc
     7201 gcagtgacag gcaactcagg cctccatcca ttttgcgaaa ctcggacata tcaagcggca
     7261 gaggcttgta tccaagaagg gacaagactt cctgcaatcg cggaaagcct gccggcacca
     7321 gcactcgatc gttgaccagc acgcaattgc aggcgtagct ctcctcgggt aaggggcgaa
     7381 tccggttgta gccggagaat tcttctctgc tggccaactc ctccataacg acaaggtcgt
     7441 ttttgtccag ataggcgatc ccgcttttga gatgcagaat cgagcgctgg tctcgaatat
     7501 cgatcatgga cgacgtgaat ccgtcggcgg cgagccattg gctgagctgc cgcgcgccct
     7561 cttggttggt gcgatgcgag atgccgataa agaagtgcga gccagcctcg cagatatctc
     7621 cgccgtcaag cgtaccgggc gcggcgatct gatgaatcga agagtagaag cgatctatgg
     7681 tctcccgaac gcccgtgact tcgcctagcc tcgaggcggc gccgggacga gtgaggatgg
     7741 ccgaacggct ggtcaggata gccgtgtcct cgacaaaagt tgaatcagga tgctgcaggt
     7801 cagccgggag ccggatcaac tgaagcccgc acgactctag ggcccggcag taagcttcgt
     7861 gctgtgacaa ggccgccgat aggtccggca tgccgagtcc ggcgctggtt agaccgtttg
     7921 caaagttgtc gcctggttta cggacgatag cgcgttgaaa caaatcaagg acctcgactc
     7981 gagattttgg gtgctggcga tgaaatcctg ggaacgccgg cgcaagccac agtacgtcct
     8041 gcgtgcccag tcattgcatt cgactctgca cctcggggga caggttgcct ccgcagaaaa
     8101 gcaccaccac agacttgccc tcgtatcgcc cagcctcctg taagtacgcg gccaatgcta
     8161 cacctgcggc accttcgatc gtccagtttt gttctcgtgc ccatttcatg gcgttgagaa
     8221 tcgcgtcctc ggaaaccaga actccacgat gcattgcggc tcgcgcgagt tcaagagtta
     8281 tcgaaccggt ctcaactcca cctgccgtgc tctccgacaa ggtgtagctc tccggatagt
     8341 caatcaccct gccggcttgc aggcactcat acagcacgcg cgagttctga ggccaacatc
     8401 ccacaatttc ggtgtgacga catctctcac gcaggtaggc tccaacccca ctgatcaagc
     8461 caccgccgcc agttgcgatg aaaacagcgt cgaggctttc aacctggtgc acgagctctg
     8521 ccccgatggt tccctgcccg gcaaccacct cccagtcgtt gtagggcgac acataggttc
     8581 gaccggattc aagggccgca gctcgggcag caacctcggc ttcgagcgga ttctgcccca
     8641 cgattcgaat ccgggcaccg taggatgcga tctttttcca cttcgcttcg ggcacttggg
     8701 ccgacagata aacttctgcg tcgattccgc gatgcctcgc cgccgcagcg acggctagtc
     8761 catgattacc cgtcgaggac gcgaccacgc caactaaggc ggcctgaggg gaaaggccca
     8821 tgaccttgtt ggccgctccc cgcaacttga aggaaccggt tagctgcaac tgctccatct
     8881 tggcgaatgc acgggtagtt gcgggtatgc gaggatcgcg cggcaatcgg gccaccggcg
     8941 tttgctgtac caggccgtaa atgcggttag tggcgtcttc aatcaacgcc aggagatccg
     9001 gagtttgagc aacttccggc gcgacattct ggccgttctg catagcagtt ctgatcttca
     9061 actgagaagc gaggacctcg aacgcaagct cgattcaggg gtaactggtg cgtcgttgcg
     9121 tcctgtttat ttccgcttgg gctcaactcc tggcgacagg acggcttcca ccggcggaga
     9181 cagcgtgcgg tttcgtagcc gctggcggca tggctgagtc ctcccaaaga cgaaagccgc
     9241 ccttgaacgc gttggcgttg tctccggccc ggcacttagc tggcatggtt ttcaggaact
     9301 tcacatttcc gccgcccagc acaacatcat ccggttccag agccgcggac aacttctcaa
     9361 ctacatcggc cacatagctc cgccatttct tcttgccaaa tcgcttcatg ccacgcagac
     9421 ctacataatc ctcataggtg cccttgcggt aaggaagatg ggccaactcc atgggaagca
     9481 gctcgccatc gatgatcatc gccgaaccta gacctgttcc cagcccaagg aaaagcatcc
     9541 ggccgcgctt gtaactgccc aaagcctgca tcgccgcgtc attcacaatc ttgaccggac
     9601 aactgaatgc tttctcgaag ttgaatccaa cccatccctt gccgagattg tggggctcgg
     9661 ccaacaggcg gccatccaga acgggcccag ggtagccaat cgagacaacg tcatacttcc
     9721 aatccgccgc gacttttcgc acttcatcga ccatctgttt cgcggtaaga gttgggccgg
     9781 aagcaaactt ccggcgagct tcgtgcccgg aggccagcaa cttaacgttg gtaccaccta
     9841 cgtcgatggc aagtactttc atccttggtc cccgttgttg cacaccgatc catggcgctt
     9901 cgcgcagcgt cgcggtgaga tcgggtcaac gttcgtcgtc gcaaaagtgt acatcacggc
     9961 ccgaagcttt cgatgttccg attgcgctca ggatcgaggt cttccggtac gcacgctgtc
    10021 ggccagttcc cggagaagtc caacggtctc gtcccatccc atgcacgcat cggtgatgct
    10081 ctgcccatgg gtaagagact tacctggaac caggctctgc gatcccgcga ccagattgct
    10141 ctccaacata acgccgatga tgcgccggtc gccactggca atttggctgg caacatggcg
    10201 gcacacggct ccttggcggg tgtgatcttt attactgttg gcatgactgc agtcgatcat
    10261 gacattgggc ttcagtccgg ctttctccag cttgccgcaa gtcgacgcga ccgcctcagc
    10321 agtgtaattg acgctgcttc ggccacctcg caggatcacg tgacagtcgg gattgccggt
    10381 cgtcgcgaag attgccgact gcccgtgctt ggtgtggccg agaaaggtat ggggatgcgc
    10441 tgcggataat acggcctcaa tcgcaatttg cacgtcgccc gaagtcgcgt tcttaaatcc
    10501 tacgggacag gagagtcctg agaccaactg gcgatgaacc tggctctcgg tggtgcgcgc
    10561 tccaatcgct ccccaactga ccaggccagc cacatattgc ggcgtgatca tgtcgaggaa
    10621 ctcagttccc gcaggcactc ccatttcggc cagatccagt agaaggtgcc gcgcctggcg
    10681 caatccatcg ttaatcttgt acgactgatc caggtaggga tcgttgatca aacccttcca
    10741 tccaatggtg gtgcgcggct tctcaaagta cacccgcatc acgatgtgga gttcttttcc
    10801 gtgctcgtcg atggcagact tcagccgggc tgcgtactcg cgcgcagctt tggtgtcgtg
    10861 gatcgaacac gggcccacga ccaccaggag gcgctggtca tcgccgcgca ggattttcac
    10921 gatctgctcg cgggcctcgt ggatggttgc cgacgccttc tccgtcgttg gcagttcttc
    10981 gtccaggaaa acgggcggta gaacgacttt agtccacttg atgcgaaggt cgtcagtcga
    11041 atagaacatg gcgttggggt cgataagaca aaaatgcaat gtgggggcga aaattgcgct
    11101 tgtaaatact ctatcagcca gcttgttcga aatctcgcgg tcggatgcaa tgagacttcg
    11161 gcatagtgtc acgaagtcat tctttgccgc gatcgagatt caaggtgacg gcctcgtacg
    11221 tccgaatcct gggagtcccg tcgatcacct ttgaaagagc cttcaagact tgtgaatagg
    11281 cgccggtatt gtaagcgtct gcatcgtgct tgttgtccca gagactgatt gcaatctgtt
    11341 ccagactgcc aggattggac aatgtgattt cgcccttaaa gcctgcctgc ttgcgaagta
    11401 aggggagtat ttcatcctcg agtgtccggg cgaactcggc agccaaggtg agcgatttca
    11461 atcggaaata ggcattacgc gcaaacactt ttgacctctt cttgacgaca gcgatgtgtg
    11521 ctacacggcc ggtactaatg aggcgacgac gagatcactt gggcttagga gcagcggcaa
    11581 ccgacttctt caccgcagcc ttggtcgtct tcgaggccgg tttgggatga gcatcctcgt
    11641 ccacttgctg ggcgacgttt gccgactcct gcgtgaaggt tgccatgacc gcgttgacgc
    11701 cgaattggtc ggcatcggct gcgactagca ggtctttcat ctccggttgg ggcttgccga
    11761 ctttgtacag ggtgacatcg gtcaaggcct ggaactttgc ggcatccagc gtcacgccct
    11821 tgggtagcga tgccggcggc aatccactgc cgggagcttt cacctgactg gccgtggtgt
    11881 agagaatgta gtcgcattgt ttggaactgg cttcgctctc gacatcttga tgaggcgctt
    11941 cggcatcgag cggtacggct tccatttggt ggtgggtgat ctcgtccatc aagtcgaggc
    12001 gcaggttgtc agtcggcagg ccctcgccgc tcgtgtcctt aatcttgacc acgccgatgc
    12061 gaaccacgcc agcgctctta ggcgcgacag tggcgacggg cgtcggtgcc ggggctgcag
    12121 gcgccttggg tgcgggtgca gccacgcctg gcgcatgact cgactccggc ggcgtattgg
    12181 gcggcgtacc actcatcatg gcggacatgt cagtaacctt gcagcccggc ggcgcgtcga
    12241 acagcggcgc gtccaacgtc gtgctggcca actccacaac ttcggtggtg gtggtgaagt
    12301 tgccatgctc cgacatgatg gtcgtagtct gcttcatggg atagcccata gcggcactac
    12361 cgccgccctt catcacgatg cggtcgttgc aactggcccg accgcccatg ccaccgcacg
    12421 ccatggcgcg gtacgactca tcggcgcagg aaaatcccgc cgagagatcg gcgtaccagc
    12481 catcggtttc catcctcatg tgagactggt tacaggcgtc gggagtggac tccatgatca
    12541 tggaagtctt gatgtgacgc gccttgtagc caaatatgtc ctggcgttcg ccggtgtcgg
    12601 tggaattacg ggtaatcgtg accacgccac ccgtgcgcgg cgcagcgggc tcatttcctg
    12661 gaccttcacg ccccatggct cgcatgtttg gcattgtggg gcacgaagtt tcgccgccac
    12721 ctgtcggaat gaccgagcat tggttgccgc tgatggtgat catgcgcttc tcgtcgcatt
    12781 gcatgatggt aaccgcgccg gcgtggcctc cgaagctcat ttcggtacgc tcgcgaggcc
    12841 ccttaatgta caccgtgctc tctgtgctgt gacccattac agtggtgcgg gtcttgatct
    12901 tcaagtcggc tgacgcgaga atagagaatc cgataatggc gaatacaaga acaacgagat
    12961 tgcgcgaagt gcggatcacc gaaggcctcc ttaagagact cgaacgattc tgcgaagaaa
    13021 acgaaggcag cgcaattcta aacaaaatgg gcaggaatac aacgggcggt gaccggatga
    13081 agcgttgcta aacgagggat tgttgaaaac gggtgaagag atttggctgc ccctcaggga
    13141 ttcgaacccc gatatgccgc tccagaggcg gctgtcctac cgttgaacga aggggcagcg
    13201 attgtgggac ctgcgcgaaa cagtggaagc tccgcccatt tattgtattg gttgtcgctc
    13261 cagagcgtca acaaacagag gcagccactc tgcttgcttg gggacctttg agtagtcgcc
    13321 gctgtgcgtc tggcacgacg gcggaaacac ttgcgcgcta gaatgagcaa catcattcgt
    13381 tctcgagagg actccatgac ttcgatcctg agtaccgctg cctttccagc cgacgcaacc
    13441 gatgtcgcac gacagccact agtggctgat cagtgcgcct tgatcgtggt tgatattcaa
    13501 gagaagttgc tgccaccgat cttcaacaag gacgaactgg tcaagaactc gcaattgctg
    13561 gtccgtgcgg ccaacgttct tgcaatgccg gtggtcgtca ctacgcaata tgcccagggt
    13621 ctcggccctg tcgttcctga tatttcgtca cttgtgaacg accttcgatc catcgacaaa
    13681 cttgagttcg gatgtttcgg cagcaatgag tttcgctcgg cgctgaagtc gctgccggga
    13741 aatcgcaata ccgtgctgct ctgcggtatg gaggcgcaca tctgcgtgat gcagaccgca
    13801 ctgggagcgc tgaatgacgg ctacctggtg catgtggcat ccgacgccat tggctcgcgc
    13861 gtgcgctgga actgggacat cgggatcgat cgcatgcgcg ctgccggcgc cgtgatctca
    13921 accaccgaga tgatgatcta tgaactacta cgttcctccg ggaccccgca attcaagcag
    13981 atgttgcctt acctgaaggg ttgatgcgaa gcgcacgctt ccggcgacgc ggcacgcggt
    14041 gtgccgtccc aatctgatga tctctctcgc tcgcaaactc cggctggctg acttcttctc
    14101 gctcggcttc ggcaccatga tcggcgtggg gtggctggtc gtgatggacg attggctggg
    14161 gcgcggcggg ccgctgggcg cgatgcttgg ctttgcggtc ggcggagccc ttctgctccc
    14221 catcggctac gtctacgggc aactggtacg cgccatgccg gatgccgccg ccgaaatcgc
    14281 gtacacgtcg gcggtgttcc cgcgctcggt aggtttcgcc accggatgga tgatgctgct
    14341 tgcctattgc atcgtttgcc cgtgggaggc ggtggcgatc ggcaggatag ccagctatat
    14401 ctttccggcc atgaactcta tcgagcttta tcgggtcgcc ggcaagcctg tatttctgcc
    14461 ccacctcgtc atcggactgg cactcactgt attgctcact gcaatgaact accgtgggat
    14521 tcggatgagc gccagcttcc agaacgtgac tacatttgga gcaatcgctc tcgtcctatc
    14581 cttcattgcg gcagggctcc ggcacggctc ggtgcagaat ttccagccat tgttcagcac
    14641 ctcggcgccg atttcaatcc tgctagtgct gcagattgtc ccgtatttca tgacgggatt
    14701 cgagtcgatc ggcaagtctt cggaggaagc tagctcggaa tttcagtcgc ggcggtttat
    14761 gttggcgatc atggcggcga tgctggtagg aatcggattc tatgtgtgcg tgattgcggc
    14821 aactgcatac gtcgctccgt ggcaaagcct ggtgcatgaa cgcttcgcga cggcggtagc
    14881 ttttgagcgc gcggtcggtt cgcgctgggt tgtcagcgtc atcacgtgtg ctgccgtact
    14941 ttccctactc gaggtgttta atggaaactt cgttgccgcc actcgcttgc tgttcgcact
    15001 gggccgacga ggattggccg attcccgctt gggctatgtg catccggtaa accgtacgcc
    15061 agcgatcgcc gttatcggca tcgggatgtt cactgcggcg tgtatgtttg cgggcgaagc
    15121 catcctggtg cccatcgctg aggtcggctc gctggcgtcg gcgctgggat ggatggcagc
    15181 ctgcgcttca tacctgcgaa tgagaccgag ctcattcggc cgggcagccg cggcctccgg
    15241 gatcgtggtc acatttctga tcctcgcgat gaagattcta ccaacggttc caggacactt
    15301 cagccgctac gaatgggcgg ctctggcatt gtggattctt ttggggctgc tgcttcgccg
    15361 gcgggccggg cacagcgaag cagagggtat ccgcgaacgc atctaggatt ccagcgcgac
    15421 gagttgcgtg taatacctcg cggcatgagg agaacttgag agcaccgtct tctccggtag
    15481 ctcgcccatg tacatggcga tctcatcgca ggcgtgcaga cgcgggcagc ggagacagtc
    15541 cttgtacatc ttgtcgggta acaactcctt gtccacaacc gtaaatccca tcttgccgaa
    15601 gaagtcggga atgcgcgtga acaagcacac gcacgtcacg ctttgacgcg cggcctcggc
    15661 cagcagcgaa tcgatcagga ggcgtccggc gccgtggcct ttcgccttgg ggccaatggt
    15721 aatcgagcgc acctccgcga gatgcattcc gtagaggtgc agcgcgccgc agcccacgac
    15781 ttcgccatcg tgctccacca ccacgaaatc acggatgttt tcgcagattt ccgccagcgt
    15841 gcgtcccagc agggttccat ctcccacgtg cacctggatg agctgatcga tcgcgattgc
    15901 gtcgggcaga gtggcgctac gcgcaaacat gggcggctcc tgcgaacatc gagagtgttt
    15961 ccttcgtctt cttcaacgcc gcccgaacct gcgcggaagc cgtaccaccc ggtacgtcgt
    16021 ggatggccag cacttgttgc aattgcaatg cttcgtagaa ctgcggacca gcctgaatgc
    16081 cacagctcgc ataatcttcc gttgacagag gctccaactc acaacccttt tcgacgcata
    16141 gctgaacggc ccttccgatc atctcgtggg cgtggcggaa gggtactcca ttgcgaacga
    16201 gaaacgccgc gcccgccagc gcgttcatga atccaccgct ggcggcttcc cgcatgcgct
    16261 cgaagtcgaa cgtcacgctc gccatgaaac cggtcgccac ccgcaacatg ctcgcgacct
    16321 gctgggtggc agcaaatacc ggatgctgcg tctcctgcat gtccttgttg taggccagcg
    16381 gaagtccctt cagggtgatg aacagcgtca tcgcatcagc aaaaaccttc gccgcgttgc
    16441 cgcgaattaa ctccagcgcg tccggattct tcttctgtgg catggcgctg ctaccggtcg
    16501 aaaattcctc cggcagcttc acgaagccga attctgtagt agcgaagatg atcgattcct
    16561 ccgcccaccg gcttaggtga agcgccacgg atgacaaagc ctgcacgaac tcaatggcaa
    16621 aatcgcgatc gctggtcgcg tcaatactgt tggcggttgg cgcgtcgaat tcgagcgcct
    16681 tcgcgatacc ggcacggtcg agcggcagga tggtcccggc caccgcaccc gatcccagag
    16741 ggcataggtt caggcgctta cggcagtccg ccagccgtcc caggtcgcgc agaaacatct
    16801 cgacataggc gagcagccaa tgggccacaa gtacaggctc ggcgcgctgc agatgcgtat
    16861 acgacggcat ggcggcatcg ccagcgctct ctgcctggct cacaaactgc tgcgcgagct
    16921 caacgagcaa ctcggagatc gcatcaacct gcgcccgcac atatagccgc aggtcggtgg
    16981 cgatttgttc gttgcggctg cgaccggtgt gcagtttgag gcccacttct ccagcaactt
    17041 ccaccagccg tttttcgacg tagtgatgca cgtcctcgat cgccggatcg tcgaccgagg
    17101 gaatcccctc ggccgcgatc tgatcgaggg ctgtcagtgt cagcgacagt tctccgctat
    17161 cgagtacccc tgccttagcg agggcggcgg cgtgtgcctt gctggccgcc acctcgtagg
    17221 ggatcaggat gcgatcgaac ggaaacgaac gctgccattg ttcgaagccg gcattctggt
    17281 cctggctgaa ccggcccgac cacatcttca cttcgctacc tcggtcttct gatgaatcag
    17341 cgcacgcgac cgtgctggga gtcccagaat gcggatgaac ccctccgcgt ccttctggtc
    17401 atagctcgtc cccatggtga agctggcgac gtcaggacgg tacatcgacg tctcggactt
    17461 gcgtcctgcg atatcgatat tccctttgta gagcgacaac gtcaccgtgc cattgatgtc
    17521 tgcctgcgag ctctttacaa aggcctgcaa cgactcgcgc aacggcgtga accatagccc
    17581 gaagtacacg agctcggcat acttcaatcc gatgtgctgc ttgaagtgcg ccaaatcgcg
    17641 gtcgagacac agcgcttcca gctcgcggtg cgctgtcagc agcagcgtcc cgcccggagt
    17701 ctcataacag ccgcgcgact tcattccgac gaagcggttc tcgaccagat cgatgcgccc
    17761 aatcgcgttg cgcgcgccaa tttcgttgag tactgcaacg atggccacag gatcaagcac
    17821 cacgccattc accgcaatgg gcaggccatg cttaaactcg atcgagactt tctcggtctt
    17881 gtcaggcgcc tcctgcggag acttcgtcca cgtccaggtg gtgggcaggg gagcgttgtt
    17941 cggatcctct agctcgccac cctcgtggct gacgtgcatc aggtttcgat cgcgactgtg
    18001 tatcttttcc cggctctgcg ccacttgaat gccatgcgac tccgcgtatt ccaagcaatc
    18061 ttcgcgcgac tttagcgtcc actcgcgcca gggcgcgatg accttcaact ctggagccag
    18121 cgcctggtag gcgtgctcaa aacgcacctg atcattgccc ttgccagtgc agccgtgcga
    18181 aaccgcagtg gcgccttcct ttagcgcaca ctccacctgg tacttcgcga tgatgggacg
    18241 cgccagcgaa gtcccgagca gatatttgtg ctcgtacacc gctcccgcct ggatcgccgg
//
LOCUS       DASRKK010000598        16061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_53551, whole genome shotgun sequence.
ACCESSION   DASRKK010000598 DASRKK010000000
VERSION     DASRKK010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53551"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1206
                     /locus_tag="VFC15_14245"
     CDS             127..1206
                     /locus_tag="VFC15_14245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZM11363.1"
                     /translation="MPSEKQVKWSQLRVGLTVIIASITLAVLIFVMTGTTGLFTKKIL
                     VRAYVDNAGGVRVGAPVRLEGVDIGNVTSIKVVHDPLRKLAPVEITMKITTKYEDTMR
                     ADCHASLNTAGVLGEVFVDVDCRAAKGGPLVNGAELPTDDVTQLQDVVRASQGTLQNV
                     DTLVKRLDNILTFIQSGQGSIGKVIYDPSLFNRANDMLTQLQQIVAEANSTNGSIGKL
                     LNSDELYNKANLAVDNLNKTIDQINSGQGTIGKFIKDPALYDNANQTMAKANQLITDI
                     NAGKGTLGKLAKDEELAKKLDVTVTRLNSILDRLDKGEGTAGKLMVDPSLYNNADQML
                     VETRNLVAAIRKDPKTYLTIHLKVF"
     gene            1503..1826
                     /locus_tag="VFC15_14250"
     CDS             1503..1826
                     /locus_tag="VFC15_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11364.1"
                     /translation="MKKTLLVLALAVGLLLPAALFADTAAWEWTTTGIEFTNGDWNFG
                     QVFTPTTNITVDFLGYFDPSTGMLDSHPVSSGMARPERWSHPRPSPLLRVSLPPTSST
                     TRLAR"
     gene            1907..2122
                     /locus_tag="VFC15_14255"
     CDS             1907..2122
                     /locus_tag="VFC15_14255"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11365.1"
                     /translation="MYAPVTYWGYNYCLGCGDVFTGTGTTNLGVGDAFWGPNFGWTST
                     PTPEPGSLMLLGTGVVGLAGMIRRKLS"
     gene            2397..4445
                     /locus_tag="VFC15_14260"
     CDS             2397..4445
                     /locus_tag="VFC15_14260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZM11366.1"
                     /translation="MPMRVFSVVLLLCAAIFCSAQSPSPSGVPKEIPSFDVTAIDKSI
                     EPCVDFYQYACGNWMKHNPVPPDKSRWGRFDELAEHNLYILRDILNHAQAPGKHTAPE
                     KMVGEFYGACMDESTIEKKGTAPLTPELAAINGVNTKADVIKQVAAMHQSGKPALFAF
                     FGQPDMHDSNATIAYLDQGGLTLPDRDYYIKDDAKSAETRQKYLEHMQKMFELAGDKP
                     DVAAAEAKTVLAVETGLATASMDRTLRRDPKTRDHKTTVTEIAAAAPNFDLTEYFASN
                     GSPKFTALNVGNPDFFKRVNSQLDAVSVADWKTYLRWKTINSEAPTLTKAFVEEDFLF
                     NGKYMSGQQEMEPRWKRCVKATDQSLGMALGQLYVDRTFGTAGKERTLTMVKAIESAM
                     KQDINQLTWMSDTTKKQAYVKLNTIVNNIGYPDQWRDYSSVTITSDDYSGNTARAGAF
                     EVKRQYAKIDKPTDRKDWSMTPPTVNAYYRPPMNDINFPAGILQPPFYGKMMDDAVNY
                     GAIGVVIGHELTHGFDDQGRKYDAQGNFQDWWTPADGKEFEKRADCTASEYSSFVAVK
                     DDKGEVKLNGKLTLGENTADNGGLKLAYMALTEIIGNTPVKPIDGYTPQQRFFLAYGQ
                     IWCQNVTDQEARKRVLTDPHSPGRWRVNGAVQNSAAFQQTFGCKAGQPMVSENACRVW
                     "
     gene            4610..6655
                     /locus_tag="VFC15_14265"
     CDS             4610..6655
                     /locus_tag="VFC15_14265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZM11367.1"
                     /translation="MRRLILMAGVVLLAGVSALAQNANEASAKPAARVSSFDLTAIDK
                     SVAPCDDFYHYACGTWLKNNPIPPDQSTWGRFNELHERNQTILRGILDKQSPPNPGRD
                     AVNQKIGDYYYSCMDEAGIEAKGTAPVKPSMDSIDGMKDKSQLPAVLGGLHHEGVKAF
                     FDFGAEPDFKDSKVDIATAGQGGLSLPDRDYYFKTDAKSVELREKLEKHIATMFELYG
                     ESPAKAAADAKTVMKVETDLAKVSLDRTEQRDPTKIYHKMSVADLQKVTPAFAWDQYF
                     TSIQTPNFDNINVAMPDFVKGMDQLIASNDLATVKTYLRWQTLRAAAPMLPKAYVEEN
                     FNFYGKTLRGAKELRPRWKRCVQYTDDDLGEALGQAYVAETFPPEAKAHTLQMVHELE
                     ASLKTDIGELSWMSPETKKQALEKLSRIDNKIGYPNQWRDYSTLNIVRGDAMGNSVRA
                     NQFEFNRQLKKIGQPVDRNEWGMTPPTVNAYYNPLENNVNFPAGILQPPFYDYKADDA
                     INFGSIGVVIGHELTHGFDDEGSQFDADGNLRNWWTPKDREQFTKLEDCFVKEYDGFV
                     VIDDVHIKGKLTLGENTADNGGLRISHMALLDVLAANPEKTLDGFTPDQRFFLGYAQI
                     WCENETAQATRLQALSNEHSDPKYRVNGVVSNSPDFAKAFGCKPNAPMVRNPACRVW"
     gene            7077..8381
                     /locus_tag="VFC15_14270"
     CDS             7077..8381
                     /locus_tag="VFC15_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZM11368.1"
                     /translation="MNSPRPYFFRILAAFAALLLLALSPACKRSVITKHEYMYVSAPE
                     TSLRDRVATLYSKVGTVRNGDRVDVLERQRRFVRVRTDSGVEGWVEQRSLVSQDVFDG
                     FQKLQQEGKAVNVQAHGTTRSELNMHLTPSRDGEHLYQLKEGEKVEVLKRATTDKNAP
                     KPPPPPKPASPGGKGQPAGKDPKPPSPPPAGGSSSPSPATSGQPTGGQSAPAPSPTPP
                     AASAAKTGAAVAGTAKVVEPPKPAMEDWYMVRNSSGQVGWVLLRMIDLDVPLDIAQYA
                     EGQRIMGYFVLNTVQETIDDQSKEMPQYLVLLNEPKDGLTWDYNQIRVFTRNRNKHRY
                     ETAYRERNMEGYFPVKTGHAVFDKEGDLPTFTIRKMFDNGQTQEVTYKMNGPIVRRVP
                     TAEELAAQKARHDSDAVARQQAVAKPASSKPKSKKKTKHKPH"
     gene            complement(8822..10054)
                     /locus_tag="VFC15_14275"
     CDS             complement(8822..10054)
                     /locus_tag="VFC15_14275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZM11369.1"
                     /translation="MALLELDFAMRLSTSPAPASSSTPAKPEPVPMLDLKRQYASIKD
                     DVAAAVERVLTTQYFIGGPELDAFERESAAYLGVRASLGCASGTDALWLALAAAGIQP
                     GDRVITSAFTFFASASSITRCGAVPVFADVDPATLNLDPAAVEKLLAGPQPVRAMMPV
                     HLYGQCADMDALPFLAQQHGFAIIEDAAQAFGASWRGKKAGSLGRAGAFSFYPTKNLS
                     AYGDGGAVSTDDEELAAHVRRLRNHGSRQRYYHEEIGWNSRLDALQAAVLRIKLQHID
                     SWNLQRRAIAARYDGLLRDSGLVKQGALTVDAKAPVGLLQTRPEAFHIFHQYVVRAFR
                     RDELRAFLTARGIGTEIYYPVPLHLQQSFADLGYKPGDLPVSEAAAKEALALPIFPEL
                     RDDELQRVVAAITEFYSH"
     gene            complement(10083..11756)
                     /locus_tag="VFC15_14280"
     CDS             complement(10083..11756)
                     /locus_tag="VFC15_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM11370.1"
                     /translation="MIRQLMFALLLATGLSAQPQSTGSQPRAATGYPIAGVVVSSVTK
                     QPLAGVTVAITATENRQSSQTIQTAADGRFAFPAVPAGKYSLTASAHGYRAQGFNQHD
                     NFFTGIAVGPDLDGTGLVFRLVPDAAIEGTVTDDDGDPVRNANVQLFQRNNDTGEQRT
                     QAFTNAVTDDRGHYQFGHLAPGIYFVAVSARPWYANYSAPGDAAANADNAARIAEERA
                     PFDVAYPLTFYPTAEDSTTASAIAIHPGERMTADVAMRAVPAVHLRIKSGEAGANNHG
                     NGLRVGFPRGFPRISQRIFEGALVPVNSAQGFGHTAGMYEYTGIAPGHYVIEMPESGG
                     KRNGAGWYKEIDLSGTVELDSAENPPLASVSGAVVLEGATRPVGKMNIVLTDRTSREQ
                     FYATVSDKGMFDFADEEIRPGTYDVELANAPGFQVKTLLAKGAKTSGRTLEIGGGGSV
                     QLVCTATRAVAKVTGVVLRDEKSFAGGMVVLVPRDPADWLLFRRDQSDSDGTFTLPAV
                     LPGTYIAIAIEDGWGLDWASPAVLQLYLKNGTPVEVTGEGNINVKVQLQ"
     gene            complement(11753..13528)
                     /locus_tag="VFC15_14285"
     CDS             complement(11753..13528)
                     /locus_tag="VFC15_14285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM11371.1"
                     /translation="MCIRNSIGIQVVSLPGRASHWVRAGSLRAQIGAVLLGMFCGALL
                     LPVRAQTSDAIRVSSAPGLPSNQARSEKYSVSGTVINAVTGEPIRKALVQVYGVQQRT
                     SFTDGDGRFQFDGMPAGQISLNAQKPGYFSEQEMRRQGQLPFAVGPNSDSVVLKLTPE
                     GVISGKVTTPTGTPLENVPLSLMYLNVREGRRHYDSKGTAVTDEDGHYRFANLLPGTY
                     YLAAEPFVPPLESVFELPPEPKTGYPGVYYPGVPDLASSSPISLSAGQQAEASFSLNE
                     APSYNISGTISGYMPNQGVNLQLCDQSGNPVSFSYEFSPENGRFDFHGVPSGVYVLKA
                     FSQSAPNQPVRTEARLNVASNVFNLHLALVPAIAIPISVRTESVAQSAPGAKRYPAPP
                     FQVPPIAVRLMATQPGSSDSYATLEGPAGAQTLILRNVEPGRYSVELMPQGIWYVQSA
                     ESGQTNLLTDELTVTAGAPVPPIEIVLRDDVATLGVIVKPSSGLDVPATIVVVPAQGS
                     NVTPRTGQYFPSKDANGGRSESQISSLAPGEYFVLAFDHIDSVEYSNPDVLKDYLPQA
                     AHATLSANQNARVTVDLIRTGEAAP"
     gene            complement(13682..15874)
                     /gene="ppk1"
                     /locus_tag="VFC15_14290"
     CDS             complement(13682..15874)
                     /gene="ppk1"
                     /locus_tag="VFC15_14290"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZM11372.1"
                     /translation="MASSAKAAPAKTSRSPRKTSSPTIARRAPHPHGIALDDPSLFIN
                     RELSLLDFQRRVLEEAQDSTNPPLDRVMFLSFVGSNLDEFFMVRVAGLKRQIEKGVTD
                     SGPDAMGPAELLRDIRASVIRLNRAAYECWLKELVPALRRSGLRIANFADLTDEQRTV
                     ADAYFQDTIFPTLTPLAFDPGRPFPHISNLSLNLAVVLRDAEGVEHFARVKIPDTLPQ
                     LVPVTVAPKLKSRTKVTAKAQVFVWIEQLVTANLAALFPGMEIVEAHPFHVTRDADSA
                     IQDLEAGDLMESVEEGVWQRRFADVVRVEINEAMSASVLEILVTNLELDRDDIYKITG
                     PLSLVRVRNLVKVDRPDLKFAPFIPALPAALLKDEEEDIFATIRRGDVLFHHPFDSFQ
                     PIVGLLQKAAKDPNVLAIKITLYRVGRNAPVVDALLEAAENGKQVAVLLELKARFDEE
                     SNIVWARKLEEYGVHVVYGLMGLKVHCKAALIVRKEGDVIRRYAHLSTGNYNSVTAQL
                     YTDIGMLTCDPDIGADCSDLFNFLTGYSAKSKYRKLLVAPISLRERLQGLIEREIEHQ
                     KGGHSGHIILKMNSLVDKKMIELLYRASQAGVKVDLLVRGICCLRPLMPGVSENIQVT
                     SIVGRFLEHSRIYYFRNGGDEEIYLGSADLMPRNLNRRVEVVFPVEDKSLIRVLKDDI
                     LGTYMKEQAKARHMQADGKYVRDPNCARKNALNSQETFIARASKRSGK"
BASE COUNT         3433 a         4802 c         4717 g         3109 t
ORIGIN      
        1 ccaacgagct gcgcatcgtg acgctggcaa caacctaatg ctatgatgcc agtgccagcc
       61 ggagtccaat cccgcatagc gtttgcgcta cttgattcgc ggtgccgaag gggcgatcgt
      121 tagaacgtgc cgagcgaaaa acaggtgaag tggtcgcaac tccgggttgg cctgaccgtc
      181 atcatcgcca gcatcacgct ggccgtcctg atcttcgtca tgaccggcac caccggcttg
      241 tttaccaaga aaatccttgt ccgcgcctac gtcgacaatg ccggcggtgt gcgcgtcggg
      301 gcgccggtcc ggctcgaggg cgttgacatc ggaaatgtca cgagcatcaa ggtggtgcac
      361 gacccgctgc gcaagctcgc cccggtagaa atcaccatga agatcaccac caagtacgag
      421 gacaccatgc gggctgactg ccacgcttcc ctgaacaccg cgggtgtgct gggcgaggtc
      481 ttcgtcgatg tggactgtcg cgccgctaaa ggcggtccgc tggtgaacgg cgccgagctg
      541 cccacggatg atgtcacgca attacaggat gtggtgcgcg ccagccaggg cacgctgcaa
      601 aacgttgaca cgctggtgaa acgcctggac aacattttga ccttcatcca gagcggacaa
      661 ggttcaattg gcaaggtcat ttacgacccg tcgcttttca atcgcgccaa cgacatgctc
      721 acccaactgc agcagatcgt ggctgaggca aactccacga atggcagcat tggcaaactg
      781 ctgaacagcg atgagctgta caacaaggcc aacttggcgg tcgataacct gaacaagacc
      841 atcgaccaga tcaatagcgg acagggcacg atcggcaagt tcatcaaaga cccggcgctc
      901 tacgacaacg ccaaccagac catggcgaag gccaaccaac tgattaccga catcaacgcc
      961 ggcaagggga ctctcggcaa gctggccaaa gacgaggagt tggcgaagaa gctcgacgtc
     1021 acggtcacgc gattgaattc catcctagac cggctggata agggtgaagg cactgcaggt
     1081 aagctgatgg tcgatccctc gctttataac aatgccgacc aaatgctggt ggagactcgg
     1141 aacctggtgg cagccatccg caaagatccc aaaacgtatc tgacgatcca cctcaaggta
     1201 ttctgataag agctgtagag ctgttaccgc ggtctcgacc tgtggggttc cggaaacaac
     1261 gccaacttta gtggcataca aaaggccttc gagtaaccgt taccaccact tagatgcaaa
     1321 gtcttgcact atctagcgtg ttcacacttg gcctctcgta gctaagtggc tgattatgct
     1381 aggctacaca acatcgcgcc actacttctg attggcaaca taactgctag atagggttcg
     1441 ttacactttc cctatacccc tgtcggtaac taacaacacg ttcgataacc aaggagaacc
     1501 gaatgaagaa gacgctactc gttctggctc tggcggttgg tctgctgtta ccagctgctt
     1561 tgtttgccga tacagctgct tgggaatgga ccacgaccgg aatcgagttc accaacggtg
     1621 actggaattt cggccaggtc ttcaccccaa ccacgaacat aacggtcgac ttcctggggt
     1681 actttgaccc ctccactgga atgctcgaca gccaccccgt aagctctgga atggcacgac
     1741 cggagcgctg gtcgcatcct cgaccatcac ctctgcttcg agtttcttta ccgcccactt
     1801 cctctacaac cagattagcc cggtagtcct gcaggcgggc cagatctacg agatcactgg
     1861 cgtcagcgga gtcgacaact acgcttatgg tgacaccggt tggaccttgt atgcccctgt
     1921 aacctactgg ggctacaact actgcttagg ttgcggagac gtcttcaccg gaaccggcac
     1981 gaccaatctt ggtgttggcg atgccttctg gggaccaaac ttcggttgga cttcgacccc
     2041 cactcctgag ccaggcagcc tgatgctgtt gggcacgggc gttgtcggcc tcgccggcat
     2101 gatccgccgc aagctgtcct aaacctccac cacgagcagg tccacagccg gagccacgcg
     2161 ctccggcttt ttcatggtgc cgaagagcct gtcgccctgt catccgcctg ccccccactc
     2221 aaggtcgccg ctcacctccc accaatttcc cagtctaagg cgtttgtagt agtgtgtctg
     2281 gttggagatt tgccattctt ccgtctccat ccgggtgcat ccagccctac ccttcgtacg
     2341 gtctcgcgcg ggcttcaaag gcgtcgcggg acaacaattc atgatgtgag gcaatcatgc
     2401 cgatgcgagt tttttcggtt gtactcctgt tgtgtgctgc aatcttctgt tcagctcaat
     2461 ctccatctcc ttccggcgtc cccaaagaga tccccagctt cgatgtgacg gcgatcgaca
     2521 agtccatcga gccttgcgtg gacttttacc aatacgcctg cggcaactgg atgaagcaca
     2581 atcccgtgcc gccggataag tcgcgctggg gccgcttcga cgaactggcc gaacacaatc
     2641 tctacatcct gcgcgacatt ctcaaccacg cccaggctcc cggaaagcac accgcgcccg
     2701 agaagatggt cggcgaattt tacggcgctt gcatggatga gagcaccatc gagaaaaaag
     2761 ggactgcccc cctcactccc gaactggcgg ccatcaacgg cgtaaacaca aaagccgacg
     2821 tgatcaagca ggttgctgcc atgcaccaga gcggcaagcc agcgctgttt gccttctttg
     2881 gacagcccga catgcatgac tcgaacgcaa ccatcgcgta tttggaccag ggcggactta
     2941 ccctgcccga tcgcgattac tacatcaagg acgatgccaa gtcggcggaa actcgccaga
     3001 aatatctcga gcacatgcag aaaatgtttg agctggcagg cgacaagccc gacgttgccg
     3061 ccgctgaagc caagaccgtg ctggcagtcg agacgggatt ggccacggcc tcgatggacc
     3121 gcacgctgcg ccgtgacccc aagacgcgcg accataaaac gacagtcacg gaaatcgccg
     3181 ccgccgctcc caacttcgat ctcaccgaat acttcgcaag caatggctcg ccgaaattca
     3241 ccgcgctgaa cgtaggcaat cccgacttct tcaagcgggt caacagccag ctcgatgccg
     3301 tttcggtcgc cgactggaag acctacctac gctggaagac catcaacagc gaagcgccta
     3361 ccctgaccaa agcgttcgtc gaagaagact tcttgttcaa tggcaagtac atgtcgggac
     3421 agcaggaaat ggagccgcgc tggaagcgct gcgtgaaagc caccgaccag agcctgggca
     3481 tggctctggg ccagctttat gtggaccgaa ccttcggcac cgcaggcaag gagcgcacgc
     3541 tcacgatggt gaaggccatc gagagcgcaa tgaagcagga catcaaccag ttgacctgga
     3601 tgtcggacac aaccaagaag caggcgtacg taaagctgaa caccatcgtg aataacattg
     3661 gctatcccga ccagtggcgc gactacagca gcgtgaccat cacgtccgac gactactccg
     3721 gcaacaccgc gcgcgcggga gcgtttgaag tcaaacgcca gtacgccaag atcgacaagc
     3781 ccaccgaccg taaggactgg agcatgactc cgccaacggt gaatgcgtac tatcgcccgc
     3841 cgatgaacga catcaacttt cctgcaggaa tcttgcagcc ccccttctac ggcaaaatga
     3901 tggacgacgc cgtgaactac ggcgccatcg gcgtggtgat cggccatgaa ctgacccacg
     3961 ggttcgacga ccagggacgc aagtacgacg cgcaaggcaa cttccaggat tggtggacgc
     4021 ccgccgacgg gaaggaattc gagaagcgcg ccgactgcac cgccagcgag tactccagct
     4081 ttgtggccgt gaaggacgac aagggcgagg tgaaactcaa cggcaagctg actctgggcg
     4141 agaacaccgc cgacaatggt ggcctcaagc tggcgtacat ggcgctgacc gaaatcatcg
     4201 gcaatacgcc ggtgaagccg atcgacggct acacgcccca gcagcggttc ttcctggcgt
     4261 atggccagat ctggtgccag aacgtgaccg accaggaagc gcgcaagcgt gtgcttaccg
     4321 atccgcattc gccgggacgc tggcgcgtca acggcgcagt acagaactcg gcggcgttcc
     4381 agcaaacctt tggatgcaag gcgggccagc ccatggtgag cgagaacgct tgccgggttt
     4441 ggtaaagctg aagcaagcaa agcggcgtcg agaagtgggc tttagccctg gtgcccgatt
     4501 ccgtgcgtcc gtggctcaag ccatttgcgg gagcggccct gtgccacttc aaacccgaat
     4561 tgcgagagag cttcaaagaa ttcagtctta tatttcacgt gaggtaatca tgagacgttt
     4621 gatcctgatg gctggcgttg ttctgctggc cggagtttcc gcgctagcac agaacgccaa
     4681 cgaggcttcc gcgaagccgg cagcccgtgt ttccagcttc gacctgaccg cgatcgacaa
     4741 gtcggtcgct ccctgcgacg acttctatca ctacgcctgc ggcacctggc tgaagaacaa
     4801 tcccatcccg cccgaccaat cgacttgggg acgcttcaac gagttgcatg aacgcaacca
     4861 gacgattctg cgcggcattc tcgacaagca gtcgccgccc aatccggggc gcgacgcagt
     4921 caaccaaaag atcggagact actactactc gtgcatggat gaggcgggaa ttgaagccaa
     4981 gggcactgct cccgttaagc cctcgatgga cagcatcgac ggcatgaagg acaagtcgca
     5041 gcttcctgcc gtgctgggcg gcctgcacca cgaaggggtg aaggcgttct tcgatttcgg
     5101 cgctgaacct gactttaagg actccaaggt cgacatcgcc accgccggac agggcggact
     5161 ttcgctgccc gatcgcgact actacttcaa gaccgacgcc aagtcggtgg agctgcgcga
     5221 gaaactggaa aagcacatag ccacgatgtt cgagctatac ggcgagtcgc cggcgaaggc
     5281 cgcggccgac gctaagacgg tgatgaaagt ggagacagac ttggcgaaag tctctctcga
     5341 ccgcaccgag cagcgcgacc cgaccaagat ctaccacaag atgagcgtgg ccgacctgca
     5401 aaaagtcact cccgcgttcg cgtgggacca gtacttcacc agtatccaga cgccaaactt
     5461 cgacaacatc aacgtagcca tgcccgattt cgtgaagggc atggaccagc tcatcgccag
     5521 caacgatctg gcaaccgtaa agacctacct gcgctggcag acgctgcgcg ccgccgctcc
     5581 catgctgccc aaggcttatg tggaggagaa cttcaacttc tacggcaaga cgctacgggg
     5641 agccaaggaa ctgcgtccgc gctggaagcg ctgcgtgcag tacaccgacg acgacctcgg
     5701 tgaggcgttg ggccaggctt acgtggccga gacatttcct cctgaagcca aggcacatac
     5761 cttgcagatg gtccacgaac tggaagcttc gctcaagacc gacatcggcg agctttcgtg
     5821 gatgtctccc gaaaccaaga aacaggcgct ggagaagctg tcgcgtatcg acaacaagat
     5881 cggctatccc aaccagtggc gcgattacag tacgctgaat attgtgcgcg gcgacgccat
     5941 gggaaattcg gtgcgcgcca accagttcga attcaaccgc cagctcaaaa agatcggtca
     6001 gccggtggac cgcaatgagt ggggcatgac tcctcctaca gtgaatgcct actacaaccc
     6061 gctggagaac aatgtgaatt tccctgcggg aatcctgcag ccgccgttct atgactacaa
     6121 ggccgacgac gccatcaact tcggcagcat tggcgtggtg atcggccacg agctcaccca
     6181 cggcttcgac gacgagggca gccagttcga tgccgacggc aacctgcgta actggtggac
     6241 accgaaagac cgggaacagt tcaccaagtt ggaggactgt ttcgtcaagg aatatgacgg
     6301 cttcgtcgtg atcgatgatg ttcacatcaa gggcaaatta acgctgggcg agaacaccgc
     6361 cgacaacggc ggactgcgca tctcccacat ggccctgctc gacgtactgg ccgcgaatcc
     6421 cgagaagacg ctcgacggct ttactcccga ccagcgcttc ttcttgggct atgcacaaat
     6481 ctggtgcgag aacgagactg cccaggccac gcgtttgcag gccttgtcga acgagcattc
     6541 ggatcccaag taccgcgtca acggagtagt ctcgaactcg cccgatttcg ccaaggcctt
     6601 cggatgcaag cccaatgcgc cgatggtccg caatcccgcc tgccgcgtct ggtaacgcgt
     6661 gggtgcggat tacatcacgg cacgactgaa caggctgcgg gaaagatcgc ttttcgactt
     6721 attttgaagg ggcatggctt cagccatgcc ggacaagccc ctttatttct gtcatccctc
     6781 gcgggctttt agcccgcggg ggatctgctc ttctgacttt ttccgcagcc tgttcagtcg
     6841 cgtcgacgag cccgttcgat tctgatcttc cgcgctgcga cgggcacgag catgcaagtg
     6901 tctgaatgca gcgaattccc ggttgccttg gttcttggct caagctttct ctgtgtcctc
     6961 cgtgcctccg tggtaggttt tgcctttgtc tttgtgcccc tccgtgcacc atctttgcaa
     7021 ctaccacgtc tcgctctgct ccgcgcacga ctgggttata gtgaaatgtg gtatcggtga
     7081 actcgccccg cccttatttt ttccgcatac tcgcagcgtt tgcagccctt ctgctgctgg
     7141 cgttgtcgcc tgcctgcaag cgctcggtca tcaccaagca tgaatacatg tacgtctcgg
     7201 cgccggagac cagcttgcgc gatcgcgtcg ccaccctgta cagcaaggtt gggacggtgc
     7261 gcaacggcga tcgggtcgat gtactcgaac ggcaaagacg ctttgtgcgc gtgcgcactg
     7321 acagcggcgt cgagggatgg gtcgagcagc gcagcctggt gtctcaggat gtcttcgacg
     7381 gctttcagaa gctgcaacag gaaggcaagg ccgtgaacgt gcaggctcac gggaccacgc
     7441 gttcggagct caatatgcat ctcactccgt cgcgtgatgg cgaacatctt taccagctca
     7501 aagaggggga gaaggtcgaa gtcctgaagc gcgccaccac cgacaagaat gctcccaagc
     7561 caccaccgcc gcccaagcct gcgtcgccgg gtggcaaagg tcagcctgcg ggtaaggacc
     7621 cgaagccgcc ttcaccgccg cctgcgggag gaagttcatc cccaagcccg gccacgtcag
     7681 gccaacccac tggtggacag tctgctccgg caccttcccc aacgcctccg gctgcatctg
     7741 ccgcaaagac cggcgcggcc gtcgccggca ccgcgaaagt tgtggagccg cccaaacccg
     7801 cgatggaaga ctggtacatg gtgcggaatt cctccggcca ggttggatgg gtgctgcttc
     7861 gcatgatcga tctcgacgtt ccgctcgaca tcgcgcagta cgctgagggc cagcgcatca
     7921 tgggctactt tgtcctgaac acggtgcagg aaaccatcga cgatcagagc aaagagatgc
     7981 cgcagtacct ggtgctgctg aacgagccga aagacggcct gacgtgggac tacaaccaga
     8041 ttcgcgtctt cacccgtaac cgcaacaagc accgttacga aacggcttac cgcgagcgca
     8101 atatggaagg ctatttcccg gtgaagaccg ggcatgcggt gtttgacaag gaaggcgacc
     8161 tgccgacctt taccatccgc aagatgttcg acaacggaca gacccaggaa gtcacctaca
     8221 agatgaatgg ccccattgtg cgccgcgtgc cgactgcgga agaactcgcg gcgcagaaag
     8281 ccagacatga ttccgatgcc gtagcccgcc agcaggctgt ggcgaaaccc gcgagcagca
     8341 agcccaaatc caaaaagaag accaagcaca agcctcattg aggcggccgg cgattttgga
     8401 aacgtaaaag actccgtgcc ctccgtgccg cagtggtgaa tccctcccga cggatatgac
     8461 gtcagaccct caacttccca tttcggaaca gcgctggttg ccttagtttt ccagccctgc
     8521 tgattccttt ggcgtttccg ctctgcacgc gctacaacct gtagggtcag gggattggtg
     8581 tctctggccg tttcacgccc atgcgtgaat cgaagggctc tttgacaatt cctgaaatgg
     8641 aacgaaagaa ccctacggcc ccgcggaatc gcaccgctaa gtccaatgtt gtgacatgga
     8701 tacgtataac tatatttaga ttcagctggt tacgaacaaa atcatcccaa gctgatgatt
     8761 ctaaacgatc gtgacaaagt aattttggtg ttttgcgcct tctcgcggca gcgccgaggc
     8821 tttagtggct gtagaactcg gtgatggctg ccactacgcg ttgcaattcg tcgtcgcgca
     8881 gttcagggaa gataggcagg gccagcgctt ctttcgcggc ggcttccgac accggaaggt
     8941 cccctggctt gtagcccaga tcggcaaagc tctgctgcag atgcaggggc acgggatagt
     9001 agatctcggt accgatcccg cgggcggtga gaaaggcgcg cagttcgtcg cgccgaaaag
     9061 cgcgcaccac gtactgatgg aagatgtgga aagcctcggg tcgcgtctgc aacaagccga
     9121 ccggagcctt ggcatccaca gtcagcgcgc cctgcttcac cagtcccgaa tcgcgcaaca
     9181 gcccgtcgta cctggcggca atcgcgcgac gctgcaggtt ccaactgtcg atgtgctgca
     9241 gcttgatgcg cagcaccgca gcctgcagcg cgtcgaggcg gctgttccag ccgatctctt
     9301 cgtgatagta gcgctggcgg ctgccatgat tccgcagtcg ccgcacatgc gctgccagtt
     9361 cttcgtcgtc ggtggagacg gccccgccat cgccataggc gctgaggttc ttggtgggat
     9421 agaaactgaa tgcgcccgcg cgtcccagcg agcccgcctt cttcccgcgc cagctcgcgc
     9481 caaaggcctg cgccgcgtct tcgatgatgg cgaagccatg ctgctgggcc agaaagggga
     9541 gcgcgtccat atctgcgcac tggccataga gatggacggg catcatcgcg cgcacgggtt
     9601 gtggaccagc gagcagtttc tccaccgcgg cgggatcgag gttgagggtc gcgggatcga
     9661 catcggcgaa gaccggaacc gctccgcagc gcgtgatcga actcgcggaa gcgaagaacg
     9721 taaaagccga agtgatgacg cgatcgcccg gctgaattcc cgcagccgcc aacgcgagcc
     9781 acaaggcatc cgtgccggaa gcgcagccca ggctggcgcg tacgccgagg taggccgccg
     9841 actcgcgctc aaacgcgtcc agctccgggc cgccgatgaa gtattgcgtg gtcagcaccc
     9901 gctctacggc agcggcaacg tcatctttga tggacgcata ctgccgcttc agatcgagca
     9961 tgggaacggg ttcaggcttg gcgggagtag aggaagaggc cggtgctggt gaggtgctaa
    10021 gccgcatggc gaagtccaat tctagcagcg ccacgcggga ttattgcggg aagtggttgc
    10081 gcttactgca actgaacctt gacgttgata ttgccttcgc cggtgacttc cacgggagtg
    10141 ccattcttca ggtacagctg cagcaccgcg ggactggccc aatcgaggcc ccagccgtcc
    10201 tcaatcgcaa tcgcgatata ggtgccgggc agcacagcgg gcagagtgaa ggtcccatcg
    10261 ctatcgctct ggtcgcgacg gaacagcagc cagtcggccg ggtctcgcgg gacgagtacc
    10321 accatgcctc cagcaaagga cttctcgtca cgcaagacga ccccggtgac tttcgccaca
    10381 gcgcgcgtag ccgtgcacac cagttgcacg cttccgccac cgccaatttc gagggtgcgg
    10441 ccagatgttt tggcaccctt ggccagaagc gttttgacct gaaatcccgg cgcgttcgcc
    10501 aattcaacgt cgtaggtacc gggacgaatt tcttcatccg cgaaatcgaa catgcccttg
    10561 tccgacactg tggcatagaa ctgctctcgc gatgtgcggt cggtgaggac gatattcatc
    10621 ttcccgactg gccgcgtcgc gccttcgagt acgaccgcgc cgctgaccga ggccagcggt
    10681 ggattttcgg cggaatcaag ttcgacggta cccgacaggt cgatctcttt gtaccaaccg
    10741 gcgccgttcc gcttgccgcc cgattccggc atctcgatca cgtagtgtcc gggcgcgatg
    10801 ccggtgtact cgtacatgcc cgcggtgtgg ccaaagccct gcgccgaatt caccggcacc
    10861 aaggcgccct caaagatgcg ttgcgagatc cgcggaaagc cgcgcggaaa accgacgcga
    10921 aggccgttgc cgtggttgtt cgctccggct tcgccgcttt tgatacgcag gtgcacggcg
    10981 ggaactgccc gcatcgcgac gtcagcagtc atgcgctcgc cgggatgaat cgcgatggcg
    11041 ctggcggtgg tcgaatcttc ggcggtaggg taaaaagtca gaggataagc gacgtcaaac
    11101 ggtgcccttt cttcggcgat gcgtgctgcg ttgtcggcat tcgcggcagc gtccccgggc
    11161 gcggaatagt tcgcgtacca tggccgcgcc gacaccgcga cgaagtaaat tccaggagcg
    11221 agatggccga attgatagtg gccgcgatcg tcggtgaccg cattggtgaa agcttgcgtg
    11281 cgctgctcgc cggtgtcgtt gttgcgctgg aacaactgca cattggcgtt ccgcaccggg
    11341 tcgccgtcgt catcggtgac ggtgccttcg atggctgcat ccggtaccag gcggaacacc
    11401 aggccggtgc cgtcgaggtc gggaccgaca gcgatgccgg taaagaaatt gtcgtgctgg
    11461 ttgaagcctt gcgcacggta cccgtgagcg ctggcggtca gcgaatattt tcccgcgggc
    11521 acagcgggaa atgcgaaacg tccgtccgca gccgtctgta tggtctgcga cgactggcga
    11581 ttctccgtcg cggttattgc cactgtgacg ccggcaagag gttgcttggt taccgagcta
    11641 accacgacgc cggcgatggg atagccggtc gctgccctgg gttgggaacc tgtggactgg
    11701 ggttgagcag agagtcccgt cgccagcagc aatgcgaaca taagctgtcg gatcacggtg
    11761 ctgcttctcc cgtgcgaatc aagtcgacag tgactctggc gttctgattt gcactgagcg
    11821 ttgcgtgggc agcttgcggc aggtagtcct tgagaacgtc cggattggaa tattccacgc
    11881 tgtcgatgtg gtcgaaagcg aggacgaaat actccccggg cgccagagat gagatctggg
    11941 attcagacct cccgccgttt gcgtccttgg acgggaaata ttgcccggtt ctcggcgtca
    12001 cattggagcc ttgcgccgga accacgacga tggtagctgg aacatcgagg ccgctcgatg
    12061 gtttgacgat cacgcccagc gtggccacgt catcgcgcaa cacgatctcg atgggaggca
    12121 cgggcgctcc cgctgtcacc gtgagttcgt ccgtaaggag gttggtctgt ccagactccg
    12181 ccgactgaac gtaccagatg ccttgcggca tcaactcgac cgaatacctg ccgggttcca
    12241 cgttgcgaag gatgagagtc tgcgctccag ccggcccttc gagggtggcg taagagtcgg
    12301 acgagccagg ttgtgtggcc atcagacgca ccgcaatcgg cgggacctga aacggcggag
    12361 cggggtaacg cttggcgccc ggtgcagatt gtgccaccga ttccgttcgc accgagatgg
    12421 gaatcgcaat cgcgggaacc agcgccaggt gcaggttgaa gacattggag gcgacattca
    12481 agcgtgcctc ggtacgaacc ggttgattcg gagccgactg cgaaaacgcc ttcaggacgt
    12541 aaacgccgga gggtaccccg tggaagtcga agcgcccgtt ttccgggctg aactcatacg
    12601 agaacgagac cgggttcccg gactggtcgc acaactgaag attcacaccc tgattgggca
    12661 tgtagccgct gatggttccc gagatgttgt atgacggtgc ttcattcagg gagaagctgg
    12721 cctcggcctg ctgcccagca ctcagcgaaa taggcgacga agaggcgaga tcgggaacgc
    12781 cagggtagta cacgccggga tagccggtct tcggttccgg gggaagttcg aatacgcttt
    12841 cgagcggcgg gacgaaaggc tcggctgcga ggtagtacgt tcctggcagc agattggcga
    12901 aacggtagtg gccgtcttca tctgtgaccg ctgtcccctt ggagtcgtaa tgacggcgtc
    12961 cctctctgac attgaggtac atcagactga gtgggacatt ctccagcggt gtcccggtcg
    13021 gcgttgtgac cttgccggag atcacgccct ccggtgtgag cttaagtacg acagagtctg
    13081 aattgggacc gactgcgaac ggaagttgcc cctgccgccg catctcctgc tcgctgaaat
    13141 accccggttt ttgtgcgttc agcgagatct ggccggcagg catcccatcg aattggaagc
    13201 ggccatctcc atcagtaaac gaggtgcgct gttgcacgcc gtacacctgc accaacgcct
    13261 tgcggatggg ctctccggtc accgcgttga tcacagttcc ggagactgaa tatttctcgc
    13321 tgcgagcctg gttggaaggt aatccggggg ccgacgaaac ccggatagca tcggaagtct
    13381 gagctctgac gggcaacaga agcgcgccgc agaacatgcc cagcagaacc gcgccaatct
    13441 gcgcgcgcag tgaaccagcg cgtacccagt gcgaagcgcg gcctgggagc gagactacct
    13501 gaatcccaat cgagttgcgg atacacatcg aaagattccg agggctgacg ccgcaacctg
    13561 agacgacgtc tagccacact ccactataga caccactgga caggatggag ttgcgattca
    13621 aacgcaacgc ccgcctcaac cccacagaca acgcggcaac tgtcaggttt gtcgcctatg
    13681 ctcacttgcc cgagcgtttg ctggcgcgcg cgatgaatgt ctcctggctg ttgagcgcgt
    13741 tctttctagc gcagttggga tcgcgcacgt acttgccgtc ggcctgcatg tgccgcgctt
    13801 tggcctgctc cttcatgtag gtgccgagga tgtcgtcctt cagcacgcga atcaggctct
    13861 tgtcctcgac tgggaagacg acctcgactc ggcggttcag gttacgtggc atcaagtcag
    13921 cgctgccgag atagatctct tcgtccccac cattgcggaa gtaataaata cggctgtgct
    13981 cgagaaagcg tcccacaatg ctggtgacct ggatgttctc gctgacgccg ggcatgagag
    14041 ggcgcaggca acagatgccg cggaccagca aatcgacttt cacgccagcc tgcgacgccc
    14101 ggtagagcaa ctcgatcatt ttcttgtcga ccagcgagtt catcttgagg atgatgtggc
    14161 cgctgtggcc gcccttctga tgctcaatct cgcgctcgat cagcccctgc aagcgttcgc
    14221 gcagggagat aggggcgacc agcagcttgc ggtatttcga ttttgcggaa tagccggtga
    14281 ggaaattgaa caagtcgctg caatcggcgc cgatgtcggg atcgcaggtc agcataccga
    14341 tgtcggtgta aagctgcgcg gtcacgctgt tgtaattgcc ggtcgagaga tgcgcgtagc
    14401 ggcgaattac gtcgccttcc ttgcgtacga tgagcgccgc tttgcaatgc accttcaatc
    14461 ccatgagccc gtagaccacg tggacgccgt attcttccag cttgcgcgcc cacacgatgt
    14521 tgctctcctc gtcgaagcgc gctttcagct ccagcaggac cgccacctgt ttgccgtttt
    14581 ccgcggcctc aagcaaggcg tccaccacgg gagcgttgcg tcccacgcga tagagcgtga
    14641 tcttgatggc tagcacgttg gggtccttgg cagctttctg cagcagtccg acgatgggct
    14701 gaaacgaatc gaagggatga tggaacaaaa cgtcgccacg ccggatggtg gcgaagatgt
    14761 cttcttcctc gtccttcaac aaggctgcag gtaatgccgg aatgaacggc gcaaacttca
    14821 ggtcgggacg gtcaaccttc accaggttgc gtactcttac cagtgacaac gggccggtga
    14881 tcttgtaaat atcgtcacgg tcgagttcga gattggtgac cagaatctcg agcactgaag
    14941 cggacatggc ttcattgatt tcgacgcgga ccacatcggc gaaacgcctc tgccagaccc
    15001 cctcttctac cgactccatc aggtcgccgg cctccaggtc ttgaatggcc gaatcggcgt
    15061 cgcgtgtcac gtggaaggga tgcgcctcaa caatttccat gccggggaac agcgccgcca
    15121 ggttggccgt caccagttgc tcgatccaga cgaacacctg tgccttcgca gtcaccttag
    15181 tccggctctt caacttcggg gcgaccgtca ccgggacgag ctgcggcagt gtgtccggaa
    15241 ttttcacgcg ggcaaagtgc tcgactcctt cggcatcgcg cagaacaacg gccaggttca
    15301 ggctgaggtt cgagatgtgc ggaaagggcc ggccggggtc gaaggccaga ggagtcagag
    15361 tggggaaaat tgtgtcctga aaatatgcgt ccgcgactgt acgctgctcg tcggtgagat
    15421 cggcgaaatt ggcgatgcga agcccggacc tgcgcagcgc tggaaccagt tccttcaacc
    15481 aacactcata ggccgcccga ttcagccgga ttacggaggc ccgaatgtcg cgcagtaact
    15541 ccgcgggccc catggcgtcg gggccgctgt cggtcactcc cttttcgatc tgtcgcttca
    15601 gtccggcaac gcgcaccatg aaaaactcgt cgagattcga gccgacgaac gaaagaaaca
    15661 tcacgcggtc gagcggcgga ttggtgctgt cctgcgcctc ctccaacacg cggcgctgaa
    15721 aatcgagcaa actcagttcg cgattgatga aaaggctggg atcgtcgaga gcgatgccat
    15781 gggggtgggg agcgcgcctg gcaattgtgg gcgatgacgt tttgcgggga gacctgcttg
    15841 tctttgcggg tgctgctttc gcggagcttg ccataaattc gtcggtgctg aaacgggcgg
    15901 aattgccact atccacattg tagtgtgcag acgactgaat tgcccatcgg gtcaagttgc
    15961 gcctcagcca caatttgcca atcattcaat cttggctgga gtgccacacc agagtgtcaa
    16021 tacagagttc gatccgtgga ctcaccaatt ttcggaaaac t
//
LOCUS       DASRKK010000599         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_738535, whole genome shotgun sequence.
ACCESSION   DASRKK010000599 DASRKK010000000
VERSION     DASRKK010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738535"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFC15_14295"
     CDS             <1..296
                     /locus_tag="VFC15_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11373.1"
                     /translation="AAMRKAFKKAGVEFLKRGKRIGVTSPPVGHVGRFTDRRSIQDNF
                     APAAAFAGVTTTDFGTVIDTAWSGVTINWDDGQTSSVRHNDMKQVERVPMKLV"
     gene            complement(334..810)
                     /locus_tag="VFC15_14300"
     CDS             complement(334..810)
                     /locus_tag="VFC15_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11374.1"
                     /translation="MKALSLGLLLAITTLSSAHAQRSPESLPAVTVDCYQSFAAKAQL
                     PEHPSKEIAIDGGSFETPLIRYRLQVVDKTTLKIIKDPNRPAFRSEHGKSVWEMKRDF
                     TNRHQIVGWREELSGGIVRLFTFDFEDLLFSTIDVSPARSPAAGVELHVMKCSKPI"
     gene            complement(1633..>2697)
                     /locus_tag="VFC15_14305"
     CDS             complement(1633..>2697)
                     /locus_tag="VFC15_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM11375.1"
                     /translation="KDAVLVIDDTAMPKKGDRSVGVAPQYASSLGKTANCQTLVSLTL
                     ARGEVPVMVALRLFIPESWTNDPVRLKRAGVPVEHRAARTKPEIALAEIDRVIAAGVR
                     FGCVLADAGYGLSAPFRQGLTTRGLVWAVGFPGRQKVYPAGVKLIFPIAGRGRPRQRH
                     IPDLLSIPAEDMLANAKWKNVSWRLGTKGRLKARFAAVRVRIADGPPQRIRDKGQQHL
                     PGEEAWLIGEHRSSGEKKYYLANLPAATDLRTLAATIKARWICEQAHQQLKEELGLDH
                     FEGRSWQGLHRHALMTMIAYAFLQHRRLAQAGRKKKNQRATTSAQPASRAPRHRRSHP
                     SATKSAMPTLQKTNQRKVTA"
BASE COUNT          606 a          733 c          761 g          597 t
ORIGIN      
        1 ttgccgcaat gcgcaaggct ttcaaaaaag ctggcgtgga atttctcaaa cgcggcaagc
       61 ggatcggcgt cacgagcccg ccggttggcc acgttggtag atttactgac cggcgatcaa
      121 tccaagacaa cttcgcgccg gcggccgcgt ttgctggcgt cacaacgacg gacttcggca
      181 cggtgatcga caccgcttgg agtggagtga ccattaattg ggatgacggt cagacgagtt
      241 cagtacggca caacgatatg aagcaggttg agcgggtgcc gatgaaactg gtgtgagggc
      301 gtgacatttc accgtcacgg aaagacaatc cgactatatc ggtttactgc atttcatcac
      361 gtgaagctct accccggcgg cgggagaacg cgcaggtgac acgtcaattg tactaaataa
      421 gaggtcttca aagtcgaatg tgaacagcct cacgattccg ccagaaagtt cttctcgcca
      481 acccacaatc tgatgcctgt tggtaaaatc ccgtttcatt tcccaaacac tcttgccatg
      541 ctcacttcga aatgcagggc gatttgggtc tttgattatc ttaagcgttg ttttatcgac
      601 gacttgaagt ctgtagcgta tcaacggcgt ttcgaaacta ccgccgtcga ttgctatttc
      661 cttggaagga tgttccggca actgcgcctt tgcggcaaac gattggtagc aatcaaccgt
      721 gacagcgggc agcgactcgg gagagcgttg tgcgtgggca ctcgacaacg tggtgatagc
      781 cagcaacaat ccaagactga gtgctttcat gttacatgct ccatgtggca actcacttct
      841 aatgtaccct ccgtgcaatc aaatcggaac acatttcatg gccgattaca tggtcgcacg
      901 agttcaattc gtcacaacga tatggcgcag gttgagtggt gtcgacgaaa ataaggtgag
      961 ggcaagtccg actgtgcccg aaggggtcgg aagcaagctg acaccgcatc agcaagacga
     1021 aatctgaagc gcaaggctga aaaaagagcc gctgggcggt ggctgttcat tttttgacaa
     1081 tgatgaaggc aacccattgt ctggcaatca gcctgtgaat tgagtggata accacaacaa
     1141 tgccgagatt ggatcattcg gcagcgaagt tcttcccgca agccccggac agactaacta
     1201 gctcatgcga ggaagaaagc cgtgacaatc catgtatcac tgacaagtct agatcgttac
     1261 gaatccgcag ttgaggatgc aattgcgaca tgtaacggag atctgcgcgg tgcgttaaag
     1321 gtactgatta tcgcgaatga atttctcgaa agggacattg agagattggc ccggaagtgc
     1381 gtagcgaact gaccgacact gaaaatcgaa tgtcgctgca tcgatgccat ggtcgataga
     1441 ccggaaactg atggtgcggt ttacgtcaca ggctggacca tatccggcct agccgtgctc
     1501 ggagccatcg ttgcagtttg ggttcttggg atttagcccg gaacgatttg cgccaccccg
     1561 attatcttgg ggtgttggtc ccccgtcggc acacatttgg tgactctgac tagcactact
     1621 ttggcagatt attcacgccg ttacttttcg ctgatttgtc ttctgcagtg tgggcatcgc
     1681 tgatttggtg gccgaaggat gagagcgacg atggcgtggc gcacggctgg caggctgggc
     1741 tgaggtggtg gcccgttgat tctttttttt ccgccccgct tgtgcgaggc ggcgatgctg
     1801 gaggaacgcg taggcgatca tggtcatgag tgcgtgacga tgaaggcctt gccaggatcg
     1861 tccctcgaag tgatcaagcc cgagttcctc tttcagctgc tgatgggcct gttcgcaaat
     1921 ccatcgcgcc ttgatggtgg cggctaacgt gcgcaggtct gtcgctgcgg gcaggttggc
     1981 gagatagtat ttcttctctc ccgaactgcg atgctcgccg atcagccacg cctcctcacc
     2041 gggaagatgc tgttggccct tgtccctgat ccgttgcggg ggtccgtcgg cgatccgcac
     2101 gcgaacagcg gcaaaccgtg ctttcagtct gcccttggtc ccaagccgcc aactcacatt
     2161 tttccacttg gcattagcaa gcatatcttc cgccggaatc gacaacagat ccgggatgtg
     2221 ccgctgacgg gggcgacccc gaccggcgat cggaaagatg agcttcaccc ccgccgggta
     2281 aactttctga cgcccaggga agccgaccgc ccaaaccagc ccacgggtcg tgagaccctg
     2341 acgaaacggc gcgctgagcc cgtatccggc atccgccaac acgcagccga accgaacacc
     2401 ggctgcgatc acgcgatcaa tctcagccag tgcgatctct ggcttggtcc gtgccgcgcg
     2461 atgttcgact ggaacgcccg cacgcttcaa acgcaccgga tcgttcgtcc aactctcggg
     2521 aatgaagaga cgtaatgcca ccatgaccgg cacttcaccc cgcgcaagcg tcagcgacac
     2581 caatgtttgg caattggccg tcttgccgag agacgaggca tattgcggag cgacaccaac
     2641 cgaacgatcg cccttcttcg gcatcgccgt gtcgtcgatg accagcaccg catcttt
//
LOCUS       DASRKK010000600         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_248298, whole genome shotgun sequence.
ACCESSION   DASRKK010000600 DASRKK010000000
VERSION     DASRKK010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248298"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..384
                     /locus_tag="VFC15_14310"
     CDS             169..384
                     /locus_tag="VFC15_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11376.1"
                     /translation="MAANLLHPAQPTRHTQAPRFCTDTFSRLTHKESPDEFRHEFKDN
                     IELEHEADGGAVWTKSKDGKTGSLALT"
     gene            644..>2125
                     /locus_tag="VFC15_14315"
     CDS             644..>2125
                     /locus_tag="VFC15_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM11377.1"
                     /translation="MPEVPKTGLRIAIYARVSTEEQREGQTIDSQVAELERFCREKGW
                     LIAGIYKDEGWSGGVMERPELDRLRDDAQKAVFDAVLINDVDRLARDVTHLGVIKRDL
                     ERKGIQVMFRKLPTETSPTYNLMVNILGSFAEFEREMISDRTRRGRRHKVEVRKLYLG
                     SNTSYGYRYTPKDRATGAEGVLEIVPDEAAAVRQMFEWVDDEGLSARRVMMRLSELKI
                     QPRKGASAWAKSSVLRILRNEMYAGTWYYNKFQCCEPRNRITSPRYRKRSKCSVRRRP
                     ASEWLPLVLPESLRIVPRERWLRVQQQLTRNYAFSSRNEKHAYLLKGLIRCGGCGAGY
                     VGDPCHGRFFYRCLARCKQVPCISESKLNDVVKHAIGRIMTNPDVILEPLRRLDCAEA
                     TEAKMQVGAAERIERNAQRMSAEEQRLLDGYRTGLISPAQLGQQLERLRAGRAALDLE
                     RAALAQHPEVPTEQIATAVVDYCAEAANKLASLTADGWRELLRA"
BASE COUNT          474 a          601 c          645 g          405 t
ORIGIN      
        1 ttgatgttgc tgtttgtttt gtcgcatagt gactttttat ttcttagtaa agtcactgta
       61 gcaattgtat tgggtttgca aagtagggaa aatttggcac aaaggccgct cggggctccc
      121 tcaggtggat aacttgtcaa aaagccgccg tcggcactgg cggtgaacgt ggcggcgaac
      181 ctgcttcatc ccgcgcagcc gacgcggcac acgcaggctc cgcgcttttg tacggacacg
      241 ttcagcagac tcacccacaa ggagagtccg gatgagttcc gccatgaatt caaagataac
      301 attgagcttg aacatgaggc agatggtggc gctgtctgga caaaaagtaa agacgggaaa
      361 actggctccc ttgccctgac atgacaagag tgccaaattt tcccgtcttg gcgacggcgc
      421 aacgatctcc tatgctgacc tccgtatgcc aaaaccaccc actccaacca aactcatggt
      481 gatttgggag accgccccca atcccgaccc tcacgccctg ctgaaggcgg ttgccatcgt
      541 gttccaccgg cgggtgccgt tatccactcc ggcggatttg acaaacacgg acaagacgct
      601 attgtgtgaa cgacccgatg attcttaacc ctattctccg aacatgcccg aagtcccaaa
      661 aaccggcctg cggatcgcca tctacgcccg agtctctacc gaggagcagc gggaagggca
      721 gaccatcgac tcccaagtcg ccgagctcga gcgcttttgc cgcgagaagg gctggctcat
      781 cgccggcata tataaggatg agggctggag cggcggcgtc atggaacggc ccgaactcga
      841 ccgtctgcgc gacgacgcgc agaaggcggt ctttgatgct gtcctgatca acgacgtgga
      901 ccggctggca agggacgtga cgcatctggg tgtgatcaag cgggatctgg agcgaaaggg
      961 aatccaggtg atgttccgca agctgcccac cgagacgagc ccgacgtaca acttgatggt
     1021 gaacattctg ggtagcttcg ctgaattcga gcgggaaatg atctccgacc gtacgcgccg
     1081 ggggcggcgc cacaaggttg aagtgcgcaa gctgtacctc ggatcgaaca cctcctatgg
     1141 ctacaggtac actccgaaag accgcgccac gggcgcggag ggagtactcg aaatcgtgcc
     1201 ggacgaggcc gcagcggtac gacaaatgtt cgaatgggtg gacgacgagg gcttgtccgc
     1261 caggcgcgta atgatgcgtc tcagcgaact caagatacaa cccaggaaag gtgcttcagc
     1321 gtgggccaag tccagcgtgt tgcggattct ccggaatgag atgtacgccg gaacctggta
     1381 ctacaacaag ttccagtgct gcgaaccgcg caatcgcata accagccccc ggtatcgtaa
     1441 gcgcagcaag tgcagtgtcc ggagacggcc cgccagcgag tggttgccgc ttgtgttacc
     1501 ggagtcgctt cgcatcgtgc caagggagcg gtggctgcgc gtgcagcaac agctcactcg
     1561 caactatgcc ttctcgtcaa ggaatgaaaa gcacgcctat ctgctcaagg ggctgatacg
     1621 gtgtggcggt tgcggagcgg gctacgtcgg cgacccgtgc catggtcgct tcttttaccg
     1681 gtgtctcgca cgatgcaaac aagtcccttg catttctgaa tccaagctga atgacgtggt
     1741 caagcacgca atcggacgca tcatgacgaa cccggacgtg atcctggagc cgctgcgtcg
     1801 tttggattgc gccgaagcga ctgaagccaa gatgcaggtg ggcgctgccg agcggattga
     1861 gcggaatgcg cagcgcatgt ctgccgagga gcaacgtctt ctggatggat accgcacagg
     1921 gctgatttcg ccggcgcagc tcggtcagca actcgaaagg ctgagggccg ggcgggcagc
     1981 cctggacctc gagcgtgccg cactagccca acatccggag gttcctaccg aacaaattgc
     2041 gacggctgtg gttgactact gcgcggaggc ggcaaacaaa cttgcgagcc taacggctga
     2101 cggctggcgc gaactcttgc gagcc
//
LOCUS       DASRKK010000601         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1128877, whole genome shotgun sequence.
ACCESSION   DASRKK010000601 DASRKK010000000
VERSION     DASRKK010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128877"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1424)
                     /locus_tag="VFC15_14320"
     CDS             complement(279..1424)
                     /locus_tag="VFC15_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11378.1"
                     /translation="MNLSRRAPLQHPLADLFAGAVDSLGAALAPASVRQYRGTARNFL
                     TYLGTDHPEVTSLDQLRRDPHILGWMAQLRSRIPPLAPVTYISRLIFLRGILAELAWS
                     AQLPGLAQLLRRQDIPRAPRRLPRALSMQQDQLLQQELLRRNDLGSNVLLLLRHTGMR
                     IGEAADLSFDCLHASGTDQWTIHVPLGKLKTERMVPVDSFVCQLVQRLRFFRSFDPLP
                     ADGRLLARSSTREALIRKLRRYWHEVCAEAGIGKRIVPHQMRHTYASEMLRSGVEFFS
                     VMKLLGHASPEMTMLYLDIALTDLQREFHLARSQPRHLAPQPNTQVASAHAGLGGVVD
                     SLLTTQHVVEMYRRNLPDGAPRRCLDRLSNRLTKILSEARKLDPPGK"
     gene            complement(1421..2467)
                     /locus_tag="VFC15_14325"
     CDS             complement(1421..2467)
                     /locus_tag="VFC15_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11379.1"
                     /translation="MKFRVVHQPTANHAHSAFRVVEQTTGREVDWVNRYLDLECLRRV
                     ADLTLRSYAQHLLHFIRWWESIHHTDAIAEDALTESTLLDYVRFQSGQQPEFSGSTIN
                     QRVAVADRALRATFPGAPHQMAPEFQVSYWRRAPMGLGRPLPAVSRLRVREPKRTIVP
                     LSVDEVARFWSSFRTSRDLAIVGLMLLQGLRSQEVRDLNREDLLLPEAQIRVPGKGNK
                     TRYLPLAPEAVQLLDHYLRLERPDVRTAALFVSLKGPARGARMTPAGLRSLFRYHRQT
                     TGVKRANPHRFRHTFASDMVRAGVSLPALMRLMGHAHIQTTLIYVHVTPLEVYQQYAR
                     AVAQHIRPIPVTPS"
     gene            complement(2464..>3176)
                     /locus_tag="VFC15_14330"
     CDS             complement(2464..>3176)
                     /locus_tag="VFC15_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZM11380.1"
                     /translation="LRQWRGAEPLLHEVARFYRVQFHRHSKAVAYLYQRGIRSPALIE
                     HMQIGYAPGGCLRGWLTQLGHSLPTLREAGLVTDVGYDAYVRRIVFPLEGNLYGRSLS
                     AAAPPHRFLPGAKGGLYSWKQAQSYPEVILVEGLFDYAVLWQAGFHNVTCSLGTHLNA
                     RQFRQLCDGTRTVYLAFDADRNGSGQQAAQCLSRSLRERGITACCVSLPDGHDPNSFF
                     VQGGDARHFQSLLEAALP"
BASE COUNT          665 a          896 c         1080 g          535 t
ORIGIN      
        1 ccagcggccc gtcatattcg acgaaataca cgcgttggcg cgcaccgtgg tgattcaggt
       61 catcggcgaa cgggcgcagg cctgaggccg gctggcgcca gggcgcgggc caggcacaat
      121 gctgttcacc taagcacatc aatgcgccgc gcggcaacgc aggaacgctt tgcctggcag
      181 agcgaaacca agcttacacg caccagcgcc atatcaccgc atcgggctct tcacgtaagc
      241 cattctacga taaccccaca tatccggcca atctctgcct attttcctgg tggatcgagt
      301 ttgcgggctt ccgagaggat tttggtaaga cggttggaga gtcggtccag acagcggcgg
      361 ggagcgccat cgggcaagtt gcgccggtac atctccacta cgtgttgagt ggtaagcaag
      421 gagtccacta ctccgcccaa gccggcgtgg gcagaggcga cctgtgtgtt cggctgaggt
      481 gcgaggtggc gcggttgcga gcgggccaga tgaaactcgc gctgcagatc agtcaaggcg
      541 atgtcgagat acagcatagt catctcgggc gaggcgtgac cgagcagctt catgaccgaa
      601 aaaaactcta cgccggaacg aagcatctca ctggcgtagg tgtggcgcat ctgatgagga
      661 acgatgcgtt tcccaatgcc cgcctcggca cacacctcgt gccagtatct gcggagtttg
      721 cgtatcaggg cttccctggt gctggaacgg gccagcagac ggccatcggc gggcaacgga
      781 tcgaacgagc ggaagaagcg aagacgttgg accagttgac acacgaagga gtctaccgga
      841 accatccgtt cggtctttaa cttgccgagc ggcacgtgaa tcgtccattg atcggtgccg
      901 gaagcgtgga ggcaatcgaa ggaaaggtcg gccgcttcgc cgatgcgcat gcccgtgtga
      961 cgaagcaaca ggaggacgtt ggagccgaga tcgttgcggc gcagcagttc ttgctggagg
     1021 agttgatcct gctgcatgga gagcgcacgg ggcaagcgtc gaggagcgcg cggaatgtcc
     1081 tggcgacgga gcagttgggc tagtccggga agttgtgcgc tccacgccag ttccgccaga
     1141 atgccacgga ggaatatgag ccggctgatg taggtgaccg gcgccagggg tggaatccgc
     1201 gagcgcagct gggccatcca gccgaggatg tgtggatcgc gtcgcaactg atcgagagaa
     1261 gttacctcgg ggtgatcggt gccgagatag gtcaggaagt tgcgcgcagt gccacggtat
     1321 tgacgcaccg aggcgggagc cagtgcggcg ccaagagagt cgaccgcgcc tgcgaacaag
     1381 tcggccaaag gatgctgaag gggagcacgg cgcgaaaggt tcacgatggt gtcaccggga
     1441 tcggccggat atgctgggcc acggcacgcg catactgctg atagacctcg agtggggtaa
     1501 cgtgcacata gatgagcgtg gtctgaatgt gggcgtgacc catcaaccgc atgagcgccg
     1561 gcaggctgac gccggcgcgc accatatcag aagcgaaggt gtgacgaaaa cgatgcgggt
     1621 tggcgcgttt cacgccggtg gtctggcgat ggtagcggaa cagggagcgt aaccccgccg
     1681 gggtcatgcg ggctccgcgg gccgggcctt tcagggagac aaacaaagcg gcggtccgca
     1741 catcgggccg ctccagccgc agatagtgat ccagcagttg gacggcttcg ggagccaggg
     1801 gtagatagcg ggtcttgttg cccttgccgg gcacgcggat ctgcgcctcc ggcagtagga
     1861 gatcctcccg gttcagatcc ctcacctctt gcgaccgcaa accctgcaac agcatcaagc
     1921 ccacgatggc cagatcgcgc gaggtacgga agctggacca gaatcgggcc acctcgtcta
     1981 cggacagtgg cacgatggtc cgcttgggct ccctcacccg caagcgactc accgcgggaa
     2041 gcggtctacc aaggcccatg ggcgcgcgcc gccagtagct tacctgaaac tctggagcca
     2101 tctgatgggg agcgcccggg aaggtggcgc gtaaggcgcg atcggcgacg gcaacgcgtt
     2161 ggttgatggt ggagccggag aactcgggtt gttggccgga ctggaatcgc acgtagtcca
     2221 gcaaggtgga ctcggtgagc gcatcttcgg caatggcatc ggtgtgatga atactttccc
     2281 accaacggat aaagtgcagc aagtgctgtg catagctgcg gagcgtgagg tccgcgacgc
     2341 ggcggaggca ttctagatcc agatagcgat tgacccaatc gacctcgcgg ccggtggtct
     2401 gctcgacgac gcggaacgcg ctgtgtgcgt gattggcggt gggttggtga acgacacgga
     2461 atttcatggc agggcggcct ccagtagcga ctggaaatgg cgcgcatcgc cgccctggac
     2521 aaagaagctg ttgggatcgt ggccgtcggg cagcgagaca cagcaagcag tgatgcctcg
     2581 ttcccggaga cttcgtgaga ggcactgcgc tgcctgctgg ccgctgccgt tgcggtcggc
     2641 atcaaaggcc agatagacgg ttcgagtacc gtcgcacagt tgccggaact ggcgcgcatt
     2701 aagatgggtt cccaacgagc aggtgacgtt gtgaaaaccg gcctgccaca acacggcgta
     2761 gtcgaacaga ccctcaacga gaattacttc cggataggat tgggcttgct tccaggagta
     2821 caagccgccc ttggcgccgg gcagaaaccg gtgaggcggc gccgcagccg aaaggctgcg
     2881 tccataaaga ttgccctcca acggaaagac gatgcgacgc acgtatgcgt cgtagcctac
     2941 gtcggtcacc aggccggcct ctcgcagggt cggcaaagag tggcccaact gtgtcagcca
     3001 accccgcagg caaccgccag gcgcgtaacc gatctgcatg tgctcgatca gcgccgggga
     3061 gcggattccg cgttggtaca gataggcgac cgccttgctg tggcggtgga actgcacgcg
     3121 atagaaacgt gccacttcat gcaacaaagg ctcggcacca cgccattggc gcagcc
//
LOCUS       DASRKK010000602         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_54165, whole genome shotgun sequence.
ACCESSION   DASRKK010000602 DASRKK010000000
VERSION     DASRKK010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54165"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFC15_14335"
     CDS             <1..208
                     /locus_tag="VFC15_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HZM11381.1"
                     /translation="FLISGQGDVIEPDTGIAAIGSGGPYAIAAATALFEHTDLPPRRI
                     AEEAMKIAGKICIYTNDKVTIEEL"
     gene            complement(293..820)
                     /locus_tag="VFC15_14340"
     CDS             complement(293..820)
                     /locus_tag="VFC15_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM11382.1"
                     /translation="MARLSYVEMEQAQPEVREVYEKVLKGKPVSFHKLLAHQPEVLKT
                     LLPFYASVGRSLERRLYEMVYIRVSMINGCRYCMQHHLAASKRVGLTREDWQHLKEGQ
                     HGNFSEKEEAALDFAEKLTRDPHSIADADLQSLKLHFSEAEIVDLDLLIGLANLTNRF
                     TDPLGADVEFPEEKI"
     gene            879..2240
                     /gene="trmFO"
                     /locus_tag="VFC15_14345"
     CDS             879..2240
                     /gene="trmFO"
                     /locus_tag="VFC15_14345"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZM11383.1"
                     /translation="MPKAIRIIGAGLAGSEAAWQCTRRGVAVELFEMRPVRSTPAHQT
                     ANFAELVCSNSLKSTTENTAPWLLKEEMRRAGSLLMDIARETAVPAGHALAVDRDKFA
                     QAVTAAIEREPLIRVLREEVTAIDDQGTITIVATGPLTSDALTREILRLSGSEQLYFY
                     DSISPIVEADSIDMSLVYMAARYDKGTADYINCPMSREEYDRFLDALLEAHSVDAKDW
                     EKLNYFESCLPIEEIARRGRDTLRFGPMKPVGLRDPRTGHVPYAVVQLRQENLRADSY
                     NLVGFQNHLKYGEQARILRLIPGLENAKFLRYGQIHRNTYINSPTLLRETLQMKQHPN
                     VLFAGQISGVEGYVESIATGLLAGMQASALALNVDPAPPPRATAFGALMNYICHAEAK
                     HFQPANITFDLLMPLDETTRRRIRDKKQRHAMVCERALAELQGWLDAQRAAVENLHTL
                     PLN"
     gene            2278..>3107
                     /locus_tag="VFC15_14350"
     CDS             2278..>3107
                     /locus_tag="VFC15_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM11384.1"
                     /translation="MSKLRAGISPCMVLRDLIRDVFQTLWGHKLRTALTMFGIAWGVI
                     SIVLMVAAGEGLRVGQAKVSQNFGRDLMIIFAGRTSLQAGGTRAGRQIEYLDSDLPVL
                     QSDSPDCEFVIPELETSRARIHSNYNAGAQTITGSYPPFQEIRSITVADGRFYDWGDI
                     KETRRVAFLGSEVKKQLFGGRNPIGENIYLNDIPYKVIGVMKTKDQDSSYDGFDINKI
                     YVPYTAMHTDFPNKPPWTLHGVDQLLVTPKSVEQHEACKGEVRAALGRLHNFDPHDKE
                     A"
BASE COUNT          633 a          995 c          887 g          592 t
ORIGIN      
        1 gttcctcatt agtggacagg gcgacgtgat tgagcccgac accggaatcg ccgccattgg
       61 cagcggtggc ccctatgcca ttgcggccgc gacggcgctc ttcgagcaca ccgacctgcc
      121 gccccgcagg atcgccgagg aagcgatgaa gattgccggc aagatttgca tctacaccaa
      181 cgacaaagtg accatcgaag agctgtaagt caagtttccc aggctcaagg ttccaggttc
      241 aagctgcgct gggctagtgt ttttccgcag tgcgggcctg gttcctgcac gatcaaatct
      301 tctcttccgg aaactccaca tccgctccca acggatcggt gaagcggttc gtcaaattcg
      361 ccaggccgat cagcagatcg aggtccacga tctccgcttc cgagaaatgc agcttcaggc
      421 tttgaaggtc agcgtcggca atggaatggg gatcgcgggt gagcttctcg gcgaaatcca
      481 gcgccgcttc ttccttctcg ctgaagttgc catgctggcc ttccttcagg tgctgccaat
      541 cctctcgcgt aaggccgacg cgtttcgatg cggcaagatg atgctgcatg cagtagcggc
      601 agccgttgat catcgaaacc cggatgtaca ccatctcgta caagcggcgc tccagcgaac
      661 gtccaacgct ggcgtaaaac ggcagcaggg tcttcagcac ttccggctgg tgcgccagaa
      721 gtttgtgaaa gctgaccggt ttgcctttca gcaccttttc gtaaacctcg cgaacctccg
      781 gctgagcttg ctccatctca acgtatgaca gtctggccat aaggttgact aaccctccag
      841 ccttataata cctgcatatc tccacctcgt ttgtactcat gccaaaagca atacgcatta
      901 tcggcgccgg actggccggc tcggaagccg cctggcaatg cacccgccgc ggcgtcgccg
      961 tggaactgtt cgagatgcgg ccggtgcgct cgacgcctgc gcaccagacc gcgaacttcg
     1021 ccgaactggt gtgctccaac tcgctgaagt caacgaccga gaacaccgcg ccctggctgc
     1081 tgaaggagga gatgcgccgt gccggttccc tgctgatgga catcgcgcgt gagaccgccg
     1141 ttcccgccgg tcacgcgctg gccgtggatc gcgacaagtt tgcccaggcg gtaacggctg
     1201 ccatcgaacg cgaaccgctc atccgcgtgc tgcgcgaaga agtcacggcg atcgacgacc
     1261 agggcacgat cacgattgtg gcgaccggcc cactcacctc ggacgcactg acgcgtgaaa
     1321 tcctccgcct gagcggaagc gagcaactgt atttctacga ttccatcagc ccgatcgtcg
     1381 aggccgattc catcgacatg tcgctggtgt acatggccgc gcgctacgac aagggcaccg
     1441 ccgactacat caactgcccg atgagcaggg aagagtacga ccgcttcctc gacgcgctgc
     1501 tggaagcgca ttcggtggac gccaaggatt gggagaagct gaactacttc gaaagctgcc
     1561 tgcccatcga ggaaatcgcg cgccgcggcc gcgacactct gcgcttcggg ccaatgaagc
     1621 cggttggctt gcgcgatccg cgcaccggcc atgttcccta cgcggtggta cagttgcgcc
     1681 aggagaacct gcgcgccgac tcttacaatc tcgtcggctt ccagaaccac ctcaagtacg
     1741 gcgagcaggc gcgcattctg cgactcatcc ccggcctgga gaacgcaaag ttcctccgct
     1801 atggacagat tcaccgcaac acctacatca acagccccac gctgctgcgc gaaacgttgc
     1861 agatgaaaca gcatcccaac gtgctgttcg ccgggcagat ctccggcgtc gagggctatg
     1921 tcgagtcgat tgcgaccgga ttgctcgctg gcatgcaagc ctccgcgctg gcgctgaacg
     1981 tcgatcccgc ccctccgccg cgcgccacgg ccttcggcgc gctgatgaat tacatctgcc
     2041 acgccgaagc gaaacatttc cagcccgcca acatcacctt cgacctgtta atgccgctcg
     2101 acgaaaccac ccgccgccgc atccgcgaca aaaagcagcg ccatgcaatg gtatgtgaga
     2161 gggcgttggc ggagttacag ggctggctgg atgcgcagcg cgcagcagtc gaaaacctcc
     2221 acacgcttcc gttgaactaa tgcgccagcg cccgcgtatt acttctcagg cggccctgtg
     2281 tccaaactga gggccggtat ttctccgtgt atggttttgc gcgatctcat tcgcgacgtc
     2341 ttccagactt tgtgggggca caagctgcgt accgcgctca ccatgttcgg catcgcctgg
     2401 ggcgtgatct cgattgtcct gatggtcgcg gcgggcgagg gcttacgcgt gggccaagcc
     2461 aaagtctcac agaacttcgg ccgcgacctg atgatcatct tcgccgggcg caccagcctt
     2521 caggccggtg gcacccgcgc cgggcggcag atcgagtatc ttgacagcga cctccccgtc
     2581 cttcagtcgg actctcccga ttgcgaattc gtgatccccg agctggagac cagccgcgct
     2641 cgcattcata gcaactacaa tgccggcgcc cagacgatca ccggctccta ccctccgttc
     2701 caggagatcc gcagcatcac tgtggccgat ggccgtttct acgattgggg cgacatcaag
     2761 gagacgcgcc gcgtcgcctt tctcggcagc gaagtgaaga agcagttgtt tggcggacgc
     2821 aatcccattg gcgaaaacat ttacctcaat gacattccct acaaggtcat tggggtgatg
     2881 aagaccaagg accaggactc cagctacgac ggcttcgaca tcaataagat ttatgtgccg
     2941 tacacggcca tgcacacgga tttccccaac aagccgccgt ggacgctgca cggcgtggac
     3001 cagttgctgg tgacaccgaa gtcggtcgag cagcatgaag cctgcaaggg cgaggtacgg
     3061 gcggcgctgg gacggctgca caatttcgat cctcacgaca aggaggc
//
LOCUS       DASRKK010000603        12804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_738817, whole genome shotgun sequence.
ACCESSION   DASRKK010000603 DASRKK010000000
VERSION     DASRKK010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738817"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..465
                     /gene="rpmH"
                     /locus_tag="VFC15_14355"
     CDS             310..465
                     /gene="rpmH"
                     /locus_tag="VFC15_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZM11385.1"
                     /translation="MPKRTFQPNRRRRSKTHGFRSRMKTKSGRAVISRRRAKGRKRVA
                     VKPGFRE"
     gene            594..1016
                     /gene="rnpA"
                     /locus_tag="VFC15_14360"
     CDS             594..1016
                     /gene="rnpA"
                     /locus_tag="VFC15_14360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZM11386.1"
                     /translation="MTSVSNAFPKSKHLLRHADFQRVYQAGRRQFTGNMTVFFLRRAP
                     VAEAVTGNSLRVGFTVGKVLGGSVERNRIKRRMREAVRTSWPAVDAPVDVVFHPRKSV
                     QKLPFAEVAGEVARGLQLAVQRAREPQPGQSAMKVERK"
     gene            1013..1357
                     /gene="yidD"
                     /locus_tag="VFC15_14365"
     CDS             1013..1357
                     /gene="yidD"
                     /locus_tag="VFC15_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZM11387.1"
                     /translation="MKPVIQFALRGYKRWISPMLPHACRFVPTCSEFAMEAVEQHGAL
                     RGSWLAAGRLLRCHPFARAGFDPVPQERRGPQHVGNFGVVPPSLVGAAFAASARDRGH
                     SNSRSLADGAVK"
     gene            1463..3277
                     /gene="yidC"
                     /locus_tag="VFC15_14370"
     CDS             1463..3277
                     /gene="yidC"
                     /locus_tag="VFC15_14370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZM11388.1"
                     /translation="MQRNPWAAITEWNVYLPEYRSPQQEESGGQSRMLLVMVAAFALI
                     LVSQFLFFKNKPNSKTAGQTAQTASAPAAVTPEGRAPATETVPSSAAAPVKAATAEVD
                     TVVENDLYKIVFTNHGAQAKSWILKKYKDEKGQPLDLVNPAAAQFGLPLGLYTYDDAL
                     RNKINSALYVAPPTGNLNAPGELTFEYSDGAVTVRKTFHFDATYVVSMETVATVNGKP
                     IQAFPTWPAGFGDQASAPSNASTRAYASARIDYYAGDKVERLAAKKISGGNTLRGPLQ
                     WAGPNDQYFAAIFLPDNPGHAAMVTFRNEIQIPKDAAKPDPNNLVKVELLGAAVGDID
                     GVTRQRLFVGPKALNVLDQIHSNLLNGQPGGPDLGHVVDFGFFSLIARPLFLWLLWTH
                     EHMASNWGVSIIILTVIINLALLPLRITSMKSALKMQKLQPQMKAIQERYKKYPLRDP
                     RRADMNAEISALYKEHGANPAGGCLPLVIQMPFLFAFYSMLGVALELRQAPFLWLHDL
                     SSPDKLFILPVLIVISTLFVQKMTPNAGMDPAQQRMMTIMMPLMLGFFSWSLASGLSL
                     YWVIGNIIAIGQQYVMNRTGLGKEMRAEMEKRARKAAK"
     gene            3291..3833
                     /locus_tag="VFC15_14375"
     CDS             3291..3833
                     /locus_tag="VFC15_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZM11389.1"
                     /translation="MPITDKIAAAKKISELLNGIIATGDFHLRYKIFVDPPAAEHDFD
                     KPVIKVELSGGDSPLVLERGGELLRAFEHVALEMLRLAPDEHDQVIFDCQNFRSSRQK
                     ELRLSANVAADRVRQSGMPYAFAPMSSRERRLLHLALRDVADLRTESAGEALQRYVVV
                     YPKDYTGNPPAPQAAPSRRR"
     gene            4035..5411
                     /gene="mnmE"
                     /locus_tag="VFC15_14380"
     CDS             4035..5411
                     /gene="mnmE"
                     /locus_tag="VFC15_14380"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZM11390.1"
                     /translation="MHLDDTIVAIATPPGRGGIGVVRLSGPEAVAIAQPMLRLSHVKE
                     LQPNRAHFGELIDPATGDRIDEVVVTFFAKPHSYTTDDVVEISCHGSPVVLRHVVEMA
                     LSLGARLAEPGEFTMRAFLNGRIDLTQAEAVRDLIDSQTLYQAQVAARQLEGALSKRL
                     QPIKQKLVELIAVTEAGVDFAEDDVSVIPDAQILDRIAEVHAPLQQLLHSFAYGKVVH
                     EGLTLAIVGRPNVGKSSLFNRLVEHERAIVTATPGTTRDLVSETVSIGGIPVRLVDTA
                     GIRDALDEAESIGVRKSMEALADADMVLVVLDAAQPFDDDEARRGDTALVSLAARGPG
                     IVVQNKIDLAAAARLLPDGGLPVVMTSALTGQGIAELRAEILRLLGGPGAQQEGGFLT
                     NVRQQKLVQDSLSALDAAVAAVPAKTPHEMILLDLYNALRPLDEITGATTADDILNLI
                     FSSFCIGK"
     gene            5597..6853
                     /locus_tag="VFC15_14385"
     CDS             5597..6853
                     /locus_tag="VFC15_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZM11391.1"
                     /translation="MALTDMRIKALKPKARRYQLTDGRGLSVEVLPSGAVAWRFRYRL
                     HGKLEKVALGHYPDVSLKDARQKRDEMAGQVARGQSPAREKQLAKLALGTNSSVREFA
                     ERYYTDVVLRSLKDPSGVRRYLDKEIFPAFGNKVLQDVTAADVQTLVFRKRDNGLEAA
                     AAAIRNLIKRMFDYAIVRGAAQVNPALALPMRFITKARSRTRTLSPDEIRVYLRCLYQ
                     SNIRRQFKLALHLILLTLVRKSELLFAKWVDVDLESGEWQVPAANSKTGKPHIVYLST
                     QAADLFRELKPLAGESEWVLPGRSSPAKPFSRNAMNQALVSMAFPMDPFTIHDLRRTG
                     STLLHEKGFSSDVVEKSLNHTIGGVRGVYNKAEYSDQRREMLQFWGDYVESLATEYRA
                     LLGLQFQDGKYLRLSTKDASGRRKPH"
     gene            7117..7938
                     /locus_tag="VFC15_14390"
     CDS             7117..7938
                     /locus_tag="VFC15_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11392.1"
                     /translation="MSANQVKSRTCRKELPDIELKARLTEMGLWEKKLSRLKYDRDAD
                     IPQKAVVEDLVDCFIKLEAVLLHERRRRVHLASLKSTRQELAAIEAEIGGAAGEEKRA
                     LEERRTHCQNSASSLQEMADASDRASSELAQEIGGDGEYVAFINRALDRLGMKPDSDL
                     RPELLSAEDSAPVPSEPPNTDRSTDADTHTARKPVAEAVKKKLNNPSEHSGMLIEEVQ
                     YAISLGRSSVYRLIECGALEKRKRRPGAAKNSRTLIMISSVLAYMEQNDIDINAL"
     gene            8068..8343
                     /locus_tag="VFC15_14395"
     CDS             8068..8343
                     /locus_tag="VFC15_14395"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HZM11393.1"
                     /translation="MHDKILRLPRVKDCTGLSRSGIYDRIAKGEFPAPIPIGARAVGW
                     LESEVEAWIAQRVAISREHNSAQKGASWDETTLTHAGRARSRADKVA"
     gene            8472..8969
                     /locus_tag="VFC15_14400"
     CDS             8472..8969
                     /locus_tag="VFC15_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11394.1"
                     /translation="MAVLLVVMLGTCSALPGKPIRRAIKREPATTAAVQQGPDAESME
                     NLFTVRERLAMAAGMTIAVTAIVWALWRYRQIRRELKQVSAEANLLQRRVAKLRNEHD
                     EKVGQLASKSGENARLRTENAQLSSERDHWQRAAREARALAAEKQYSVDELRKALVSD
                     VRKDS"
     gene            8966..9829
                     /locus_tag="VFC15_14405"
     CDS             8966..9829
                     /locus_tag="VFC15_14405"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HZM11395.1"
                     /translation="MIHSSGRDKNPKGSGMGSANLTTKSVGRQCCPHKVILEPTKDFA
                     LLKRVRDCRFITSNQLFEFAKASDIANSLGSFYWRIGRLVGCGLVQTVTLQIGKYTIY
                     TITRQGLRELENRQECLLSLTSKARVLAKRDEIPHALLLNDIRRTFEQQFPVKWWRTD
                     LQVRAVNLATDKYAKDYDAVFSVDRSESGESSQTIAVEYERTLKAEDRYAEISNALSG
                     ESSVNILFYLCASADMVPLLAQRIRVKNLLLCFTVARSLIHHGKDCPVFIWTQQNLQP
                     IPMVDIINPSK"
     gene            10444..10653
                     /locus_tag="VFC15_14410"
     CDS             10444..10653
                     /locus_tag="VFC15_14410"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZM11396.1"
                     /translation="MASVIVKNPAVLSGEPVFRGTRVPFKALTDYLEGGESLAEFLEQ
                     YPSVTREAAIAALEEARDSLMAHLG"
     gene            10650..10982
                     /locus_tag="VFC15_14415"
     CDS             10650..10982
                     /locus_tag="VFC15_14415"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HZM11397.1"
                     /translation="MKILIDECAPKALKVALAASGLDCTTVQEAGWSGKENGELLALA
                     DASFDVVVTIDRNLRYQQNLTGRKIALLIVRARSNRVVDLEPHFPACAEALQTIQPGT
                     VVEVGSSA"
     gene            complement(11166..11429)
                     /locus_tag="VFC15_14420"
     CDS             complement(11166..11429)
                     /locus_tag="VFC15_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11398.1"
                     /translation="MPATRTIRRRFGLKNALDYLVSEKLLNFAEAAERHSEFAAELPR
                     FQAAVWEVFHPYDLAGYLASLRPLPRKKLQKLLYLRGAARVAG"
     gene            complement(11660..11887)
                     /locus_tag="VFC15_14425"
     CDS             complement(11660..11887)
                     /locus_tag="VFC15_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11399.1"
                     /translation="MAISASPAAPEMAVRFALAVDVSADELLHPKAAKKSTKKPGLKV
                     MRRMEQIEQLPPAAQIAVLRTIDAFVRSAER"
     gene            11994..12572
                     /locus_tag="VFC15_14430"
     CDS             11994..12572
                     /locus_tag="VFC15_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11400.1"
                     /translation="MKERVVFEANVPVTATLAYADGLKVQGRFGDQVMYSLTDGRVMY
                     VPPMVRDKLVELGVRQNEPFAICRAERREGNRRFVEWIVQADGSAAPAQSTPAHSDTP
                     GSPVNGDSGARGKANGNGNGNGNGKLASASPSSGIAEAALRAALTASIDAALAAEQYA
                     SAHGLSIRFGSEDLRAMALSLFIQHARDGGVR"
     gene            12569..>12804
                     /locus_tag="VFC15_14435"
     CDS             12569..>12804
                     /locus_tag="VFC15_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZM11401.1"
                     /translation="MTDVAVTETLGEALSPSQVNTYMTCPAKWYFRYLIGLSEPTTGA
                     LALGKAFHGTLAWYFRQKLSTGRDMKAEELREVFV"
BASE COUNT         2757 a         3691 c         3817 g         2539 t
ORIGIN      
        1 ttgccgcaac ggccgctggt cgctgagatc gagcccggac gaaaatcgcc aagcgtggaa
       61 atttcggcca gcacctcgga acgacgcttc tctaactcca acagggaatc ggacatggaa
      121 aacagacctc ctgtagccga gcgtattata tacgctcggc tacaagggac aaaaaagaaa
      181 acctccattc gttccacttt catgtcgcac gccctggaag agacttcagt ttgccagtca
      241 cccctgtcga acggtatact tttggtttgc cctatctaaa cttttagcca gtgaggagcg
      301 cagtttcgaa tgccgaagcg cacattccaa cccaaccgtc gtcgccggtc caagacgcac
      361 gggtttcgtt ctcgcatgaa gaccaagagc ggacgggcgg taatttcccg ccgtcgcgcc
      421 aagggacgca agcgcgtcgc cgtaaagcca ggcttccgcg agtagttgct taactctgtt
      481 ttctgcgcgc cctgctgcat ttccagccca cggtagcatg gagtacgagg acccgcccga
      541 tcccattgac tgcggtgtgt tgactcagcc gggaccggtg ccctatgcag gttgtgacct
      601 ctgtcagcaa tgcgtttccc aagtcgaagc acctgttgcg gcatgcggat tttcagcgcg
      661 tttaccaggc gggacgtcgc cagttcaccg gtaatatgac ggtgtttttc ttgcgtcgcg
      721 ctccggtagc cgaagctgta actggtaact cgttgcgtgt aggattcacg gtgggcaagg
      781 tactgggcgg ttcggtcgag cgcaatcgca ttaagcgtcg tatgcgggaa gccgtgcgaa
      841 cttcgtggcc cgctgtcgac gctccggtgg atgtggtgtt ccatccccgt aagtcggttc
      901 agaagttacc attcgccgag gtcgccggtg aagttgcgcg aggactgcaa ttggccgtcc
      961 agcgcgctcg cgaaccgcag ccaggtcaat cggcgatgaa ggtggaacgg aaatgaagcc
     1021 ggtgattcag ttcgcactgc gcggctacaa gcgatggatc tcgccgatgc tgccgcatgc
     1081 ctgccgcttc gttcccacct gctcggagtt cgccatggaa gcagtggaac agcacggcgc
     1141 actgcgcggt tcatggctcg cggctggacg cttgttgcgc tgtcatccgt ttgcgcgtgc
     1201 gggcttcgat ccggtgccgc aggagcgtcg cggtccgcag cacgttggta actttggagt
     1261 cgtgccgcca tcgttggttg gagcggcgtt cgccgcatcc gcaagagatc gcggccactc
     1321 gaattcccgt tcgcttgcgg acggcgcagt gaagtaagtc tggccgctca gggtcgaccg
     1381 agcgtgcgta gcatcagcac agcgtcggtc gcgatgcaaa acaatcggtt cggcgccctt
     1441 gcagcgcgcc cgccggttgg ccttgcaacg taacccttgg gccgctatta cggaatggaa
     1501 cgtctacttg cctgagtatc gcagtccaca gcaggaagag tccggcggcc agagccgcat
     1561 gcttctggtc atggtggccg cttttgcgtt gattctcgtc agccagttct tgtttttcaa
     1621 gaacaagccc aacagcaaga ctgcgggaca aactgcgcaa accgccagtg ctcctgcggc
     1681 cgtcaccccc gaaggccggg ctccggcgac tgaaacagtt ccgtcgtccg cggcagctcc
     1741 cgtcaaggca gccaccgccg aagtcgacac cgtggtcgag aacgacctgt acaagatcgt
     1801 tttcaccaac catggcgcgc aagccaagtc gtggatcctg aaaaagtata aagacgagaa
     1861 gggccagccg ctcgatttgg taaacccggc tgccgcccaa ttcggactgc cactgggcct
     1921 gtacacgtac gacgacgcac tgcgcaacaa gatcaactca gcgctgtacg tcgcgccccc
     1981 cactggcaat ctcaacgccc ccggcgagct gacgtttgag tattccgatg gcgcagtgac
     2041 cgtccgcaag acgtttcact ttgatgcgac ttacgtagtg tcgatggaaa ccgtcgcgac
     2101 cgtgaacggc aagcccattc aagctttccc cacgtggcct gccggatttg gcgaccaggc
     2161 ctcagcgccg tcgaatgcgt ccacgcgggc ctatgcgtcc gcacgaatcg actactatgc
     2221 cggcgacaag gttgagcgct tggcggccaa gaagatcagc ggtggcaata cgctgcgcgg
     2281 accgctgcaa tgggccggcc cgaacgacca gtattttgcc gcgatcttcc tgcccgacaa
     2341 tcccggccac gccgcgatgg tcaccttccg caatgagatt caaattccta aggacgcagc
     2401 caaacccgac ccgaacaacc tcgtcaaagt tgaactcttg ggcgcggcgg tgggcgacat
     2461 tgacggcgtc acccggcagc gcctcttcgt cggccccaag gccctcaacg ttctggacca
     2521 gatccactcg aacttgttga acggccaacc cggaggtccg gacctcggtc acgtggtcga
     2581 tttcgggttc ttcagcctga tcgcgcggcc gctgttcctg tggctgctgt ggacgcacga
     2641 acacatggcc tcgaactggg gcgtgtcgat catcatcctg acggtcatca tcaacctggc
     2701 attgctgcct ctgcgcatca ccagcatgaa gtctgcgctg aagatgcaga agctgcagcc
     2761 tcagatgaaa gccatccagg agcggtacaa gaaatatccg ctgcgcgatc cccgccgcgc
     2821 cgacatgaac gccgagattt cggcgctgta caaggagcat ggcgcgaatc cggcgggcgg
     2881 ctgtttgccg ctggtcatcc agatgccgtt cctgttcgcg ttttactcga tgctgggagt
     2941 cgcactcgaa ctgcgccagg cgccgttcct ctggctgcat gatttgtcct cgcccgacaa
     3001 gctcttcatc ctgccggtgc tgattgtgat cagcacgctg ttcgtgcaga agatgacgcc
     3061 caacgccggc atggacccgg cgcaacagcg catgatgacc atcatgatgc cgctcatgct
     3121 gggctttttc agttggtcgc tggcatcggg actgagcctt tactgggtca taggcaacat
     3181 catcgccatc ggacagcagt acgtgatgaa ccgcactggc ctcggcaagg aaatgcgcgc
     3241 cgagatggaa aaacgcgccc gcaaggccgc aaagtaagag gcactctgct atgccaatca
     3301 ccgacaagat tgccgccgca aaaaagatca gcgaactgtt gaacggaatc attgccaccg
     3361 gagatttcca tctccgctac aagattttcg tcgacccgcc ggctgccgag cacgacttcg
     3421 ataagcctgt catcaaggtt gaactttccg gcggcgacag tccgctggtg ctggaacgtg
     3481 gcggcgaatt gttgcgcgcc ttcgagcatg tcgccctcga gatgctgcgt ctcgcacccg
     3541 acgagcacga ccaggtcatc ttcgactgcc agaacttccg ctcgtcccgc cagaaggaac
     3601 ttcgtctctc ggccaatgtt gctgccgacc gcgtacgcca gagcggcatg ccctacgcct
     3661 tcgctcccat gtcttcgcgc gagcgccgtc tgctacatct agcattgcgc gatgtggcag
     3721 accttcgtac cgaaagcgca ggtgaagcat tgcagcgcta cgtcgtggtc tatcccaagg
     3781 attacaccgg caaccctccc gcgccgcagg ctgcaccttc gcgtcggcgg taggtcgttc
     3841 accacggaga cccggagggc acggagaaga tctgggtctt tgtcttttcg ccgatgggtc
     3901 tttcttcatt ctcgtgcaaa ctcagtaatg gtccgccaag tatccacctc ggcaggtcca
     3961 ctgccggttt tgtttttgtg acctgacttg ttcttctccg tgtcctccgt gcctctgtgg
     4021 tgaataatct ttccatgcac ctcgatgaca ccatagtcgc catcgccact cctcccggac
     4081 gcggcggcat tggtgtggtg cgtctctccg gcccggaggc agtggcaatc gcgcaaccga
     4141 tgctgcggtt gtcgcacgta aaagagcttc aacccaaccg cgcccacttc ggcgaactaa
     4201 tcgatcccgc cacgggcgat cgtatcgatg aagtcgtcgt caccttcttc gccaagccgc
     4261 attcgtacac gactgacgat gtcgttgaaa tttcctgcca cggttctcca gtcgtcctcc
     4321 gtcatgtggt cgagatggcg ctatccctcg gagcgcgcct ggccgagccc ggcgagttca
     4381 ccatgcgcgc attcctcaac ggacgcatcg atctcaccca agccgaagcc gtacgcgacc
     4441 ttatcgactc gcagacgctc tatcaggcgc aagtcgccgc ccgccaactc gaaggagcgc
     4501 tgtccaagcg cctgcagccc atcaagcaga aactagttga gctgatcgcg gtaacggaag
     4561 ccggcgtgga tttcgcggaa gacgatgtct ccgtcattcc cgacgcgcaa atcctggacc
     4621 gcatcgctga agtgcatgct cccctccagc aactgctcca cagcttcgct tacggaaaag
     4681 ttgtgcatga aggcctgacg ctggccatcg tcggccgtcc caacgttggc aagtcaagct
     4741 tgtttaatcg cttggtcgag catgagcgcg ccatcgtcac cgctactccc ggcaccacgc
     4801 gcgacttggt gagcgagaca gtttcaatcg gtggaattcc tgtgcgcctg gtggacaccg
     4861 ccggcattcg tgacgccctc gacgaagccg agagcatcgg cgtccgcaag tcgatggaag
     4921 cgctggcaga cgcagacatg gttctggtcg tgctcgatgc cgcgcaacca tttgatgatg
     4981 acgaagcccg ccgcggcgat accgcactgg tttcgttggc agcgcgaggt cctggcatcg
     5041 tagtgcagaa caagatcgat ctcgccgccg ccgcacgcct gctccccgac ggaggcctgc
     5101 ccgtggtcat gacttccgcg ctcactgggc agggcatagc cgagctgcgg gccgaaattc
     5161 ttcgtctgct aggtggccct ggcgcgcagc aggagggtgg ctttctcacc aatgtccgcc
     5221 agcagaagct ggtgcaggac tccctctctg cgctcgacgc agcagtcgcc gctgtccccg
     5281 ctaaaacccc ccacgaaatg atcttgctcg acctctacaa cgcgctgcgt ccgctcgacg
     5341 aaatcaccgg cgccaccacc gccgacgaca tcctcaactt gatcttcagt tcgttttgca
     5401 tcgggaaata acatctcctc cagtaccgtc ccatgaaatg taataatcaa tttaactcat
     5461 attgtaatca tacgattaag atcctatact atcctgcggt ggacacacaa agccgaggta
     5521 tcgtgtgtcc accggtgtgt ccaccggagc gatataaccc aaaatttggc gtcggtggac
     5581 acagtttggg ggcagaatgg cactcacgga tatgagaatc aaggccctga agcccaaggc
     5641 gaggcgctac cagctcacgg acggacgcgg cctaagcgtg gaagtccttc ccagcggagc
     5701 ggtggcttgg cgcttccgct accgcctcca cggcaagctc gaaaaggtag ctctggggca
     5761 ctacccagac gtgagcctta aggatgcgag gcagaagcgc gacgagatgg ccggacaggt
     5821 ggcgcgaggc caatccccgg cgcgggagaa gcagcttgca aagctggcgc ttgggactaa
     5881 cagtagcgtg cgcgagtttg cggaacgcta ttacacagac gtggtgctca ggtctttgaa
     5941 ggacccaagt ggcgtccgtc gctacctcga caaagagatc tttccagcgt ttggcaacaa
     6001 agtgctccag gacgtgacag ccgcagatgt gcaaacactg gtcttccgca agcgggacaa
     6061 cgggctggag gccgcggcag ccgccatccg caatctcatc aagcggatgt tcgactacgc
     6121 gatcgtgcgc ggagcggcac aagtgaatcc cgcgctggca ttgccgatgc gatttatcac
     6181 gaaagccagg tccagaaccc gcacgttgtc gcccgatgaa atccgggtgt acttgcgctg
     6241 cctctatcag tcgaatatca ggaggcaatt caagctagcg ctgcacctga tcctgcttac
     6301 gctggtgcgc aagtccgagc tgttgtttgc caagtgggtt gatgtggacc tggagagcgg
     6361 cgaatggcaa gttccggcgg cgaactcgaa aaccggcaag ccgcacattg tctatctctc
     6421 cacccaggcg gctgacttgt ttcgcgaact gaagccattg gcgggtgaat ctgagtgggt
     6481 attgccgggc cgcagcagcc ccgcaaaacc attctcgcgc aatgcgatga accaggcact
     6541 tgtcagcatg gcattcccga tggatccgtt cacaattcat gatctgcgac gcacaggctc
     6601 gactctgctg cacgagaagg gtttctcctc tgacgttgta gagaagtcgt taaatcatac
     6661 gattggagga gtgcggggtg tctacaacaa ggcggaatac tccgaccagc gcagagagat
     6721 gctgcagttc tggggcgact atgtagagag tcttgcgacc gagtatagag ctctgctggg
     6781 gctgcaattc caagatggga aatacttgag gttgtcaacg aaagacgcga gcgggcgaag
     6841 aaaaccgcac tgaataccga cttgcaggat gataagcggc ttggttgatc tagaaaatca
     6901 acgagtgcac agggacaatc gatcgcgagc ggcaggctct cgcagcactc cggacgcgag
     6961 ttgtgccttg tgggtccacc gactgtcccg gattgtggcc accggagcag ggtgccttgc
     7021 tcgcgtcgat gtcaccgtgc aacctctgca gttgaataag tcgtcatacg cgactacagt
     7081 atgtgcgttt ggaacgagtc aaggcatcat gtagcaatgt ccgcgaacca ggtcaaatct
     7141 cgaacgtgca gaaaagaact cccggacatc gagcttaagg cgcgcttgac ggaaatgggg
     7201 ttgtgggaaa agaagctaag tcggctcaag tacgaccgcg atgcggatat cccgcagaag
     7261 gccgtagtcg aagaccttgt tgattgtttc ataaaactgg aggcggtttt actccacgag
     7321 cggaggcgca gggtgcacct cgcgagcctg aagtcgaccc ggcaagagct agcggccatc
     7381 gaagcggaga taggtggtgc cgcgggcgaa gaaaagcggg ccttggagga gagaagaact
     7441 cattgtcaga actctgcctc ttctctgcaa gagatggcgg atgcgtccga tcgagcctcc
     7501 tcggagctgg cacaggaaat cggcggcgac ggggagtacg tcgcatttat caatcgagcc
     7561 cttgatcgcc tgggaatgaa gccggacagc gacctccgtc cagaactgct ttcggctgaa
     7621 gactctgccc cggtacctag cgaaccgcca aacacagacc gctccaccga cgcggacacg
     7681 cacacggcgc gcaagcccgt cgcagaagca gtgaagaaaa agctcaacaa tccatcggag
     7741 catagcggca tgctgattga ggaggttcaa tacgcaataa gcctgggacg ttccagtgtg
     7801 tatcgcctta tcgaatgtgg ggcacttgag aaacggaaga ggcgaccagg cgctgccaag
     7861 aactcgcgta ctcttatcat gatttccagt gttctcgcgt acatggaaca gaacgacatt
     7921 gacataaacg ctctctgacc aacagcctga tcgctgcact gtctcatcag ctttccgccc
     7981 gttatcccac gccgtcctac atactgtccc atcagtccta gactcttcgg cgggccttcg
     8041 tgcagcattg acgccggagg tgcggcaatg cacgacaaaa tactgcgact gccccgtgtc
     8101 aaagattgca ccgggctctc acgaagtgga atttacgatc ggattgcaaa gggagaattt
     8161 cccgcgccga tccccatcgg tgcacgggcg gtcggctggc ttgaatctga ggtggaggca
     8221 tggatcgcgc aacgggtggc gatcagtcgc gaacacaaca gtgcgcaaaa gggagcgtca
     8281 tgggacgaaa caacgctgac tcatgccgga agagctcggt cgcgagccga caaggtcgct
     8341 taggcaaggt tacggctacc atcacgaagg gccgatgctt cgaattggaa gacggcgtgg
     8401 gctgggagag agcgcttgag atcccatggt cgcgcacttc cggcagcaaa aggtggaatc
     8461 gggggccgcg ggtggctgtg ctgctcgttg tcatgctggg gacgtgcagc gcgttgccag
     8521 gcaagccgat ccggagagcc atcaagaggg agccagcgac gaccgcggcg gtgcagcagg
     8581 gacccgacgc tgaatccatg gagaacctct tcacggtacg cgaacgacta gcgatggcgg
     8641 ctgggatgac gatagctgta acggccatcg tatgggcatt atggcggtac cggcaaatcc
     8701 gtagagagct gaaacaggtt agcgcagaag ccaacctgtt gcagaggaga gtcgccaaac
     8761 tgcgtaatga acacgacgag aaggtggggc aactcgcgag taagagcggg gaaaatgcac
     8821 ggctgagaac tgagaacgcg cagctaagta gcgaacgaga ccattggcag cgggcagccc
     8881 gagaggcacg agctctcgct gccgagaagc agtattcggt agacgagcta agaaaggctc
     8941 tagtgagcga tgtccggaaa gattcatgat tcattcctct ggtcgtgaca agaatccgaa
     9001 aggctcagga atggggtcgg ctaacttaac gacgaaatcg gttggcagac agtgctgccc
     9061 tcacaaagtc attttggagc ccaccaaaga ctttgcgctg cttaaaaggg tacgagactg
     9121 ccgcttcatt accagcaatc aattgtttga gtttgcaaag gcttccgaca ttgcaaacag
     9181 tcttggatcg ttctactggc gcatcggtcg cctagtgggg tgcggcctgg tacagacggt
     9241 cacgcttcaa attgggaagt acacgatcta cacgatcacg aggcaagggc tgagggaatt
     9301 agagaacagg caagaatgcc tgttaagtct cacgtctaaa gcgagagtcc tggctaaacg
     9361 agacgaaatc ccgcatgccc tcttgctgaa cgacatccgg cgaacgtttg agcagcagtt
     9421 cccggtcaag tggtggagaa cggaccttca ggtccgggcc gtcaacctgg cgaccgacaa
     9481 atatgcaaag gattatgacg cggtattctc ggtggatcgt tcggagtctg gcgagagctc
     9541 tcagacgata gcggtggagt atgagcgcac gctcaaggcc gaagacaggt acgctgaaat
     9601 cagcaacgct ctcagcggcg agagctccgt taatatcctt ttttatcttt gcgcgtccgc
     9661 cgacatggta ccgctgctgg cgcagagaat tcgtgtgaaa aacctattgc tttgcttcac
     9721 agtcgcaagg agtcttatcc atcatggtaa ggattgccct gtattcatct ggacccagca
     9781 gaaccttcag ccgattccca tggtcgacat aatcaatccc tcaaagtgac aagcggctta
     9841 tccgatcaat tgttgttcct ggaatgagac tagaatacgc cctgaaacgg ctagaatcgg
     9901 ctgctcataa gatcctgaga gaaggcgtct tagcggctga cttacctccc tataacgctg
     9961 ctttgcgaga gcaagctttg gacggcgccg ttccgggagc agccgggtcg caaacctgcg
    10021 gttcaaaacg cccgagctgc cgaaatgcct gacgacgttc ttccatccgc gcgccgtgat
    10081 tgaaattgct tggattggat tgcctcgaca ggaccgagca gttagcacac tggcgcgcca
    10141 ccgcacgcgc gcacgatggg tgctgcggtg gtgacgcagc tcttgttcct ggctgtgcgc
    10201 cggttccggc gattgcgttc cgacagttgc tcgactctgt gcgcgagttt cgcgcggcca
    10261 gcgcgtgcgg aaccgatccg gagtacaccc ggggtacaat ctatggcgta tcgttgggca
    10321 ggcgaacttg aagaaaacaa agcaagtgga tttttgcggt gatcgaaagg gccaaggtcg
    10381 ggtgcgggta atctgcgttg cagcgcgggt gcgctaccat agcaggcagg tgggaggagt
    10441 tccatggcaa gcgtgatcgt gaaaaatccc gccgtgctca gcggcgagcc ggtgtttcgt
    10501 gggacgcggg ttccgttcaa agcgttgacg gattatctgg aaggcggcga gagtctggcc
    10561 gagtttctgg agcagtatcc gagcgtgacg cgcgaggcag cgattgcggc gctggaagag
    10621 gcgcgggaca gcctgatggc gcatctggga tgaaaattct gatcgacgag tgcgcgccca
    10681 aggcgttgaa agtagcgctg gcggccagcg gtctcgactg cacgacagta caggaagcag
    10741 gatggtcggg gaaagaaaac ggcgaactgc ttgccttggc cgacgccagt ttcgatgtgg
    10801 tggtgacgat cgaccggaat ctccgttatc agcagaacct caccggtcgc aagatcgcgc
    10861 tgctgatcgt gcgtgcgcgt tcgaatcgtg tggtggattt agagccgcac ttccccgcct
    10921 gcgccgaggc gttgcaaacc attcaaccgg gcaccgtcgt cgaggtgggg tcctcggcgt
    10981 aggcgtcttg ggagaagcag cgttcccatt ttttcgcaga agagtgtacc gagcgcggga
    11041 gccgagtcaa gggtctccgc ttcgctccga tgatcgcgcc aaaaccgcgc ccttgacacg
    11101 gctccctctc cgctctgttt gcttccagcc atcgaaaaaa tgtgagaaac aaatgggagc
    11161 cggggttagc cagccacacg cgcagcaccc cgcaagtaga gaagtttctg gagcttcttc
    11221 cggggcaggg gacgcagaga agccagataa ccggcaaggt cgtagggatg gaagacttcc
    11281 cacacggcag cctgaaatct gggaagctca gcggcaaact cggaatgccg ttcggcggcc
    11341 tcggcgaagt tcagcagttt ctcgctgacc aaataatcca gtgcgttttt caagccgaag
    11401 cgccgccgga tggtgcgggt tgctggcatt gctatttcca aatcttgtag agctgcatga
    11461 gggagactat agcgccgtcg tgcgttgttc gccaagctga gccaacaaca actcggagca
    11521 cgacatcatc ccttgcggcc tgcggggcaa ctacggcgca cgggcgatag catcgctgca
    11581 tcggctgtac gtccgggctt tgttcggtga ctatccggcc tgcgtccggt accggttcgg
    11641 tgttcggtcg attgtctgct tatcgttctg ccgagcggac aaacgcatca atggttctga
    11701 gcacagcaat ctgtgcggct ggtgggagct gttcgatctg ctccatccgg cgcatgacct
    11761 tgaggccggg tttcttcgtg ctcttcttcg ctgccttcgg gtgcagcagt tcatcagccg
    11821 aaacatcgac ggccagggcg aagcgcacgg ccatctccgg cgcagcggga gacgccgaga
    11881 tcgccatctc caacttttta ccacgccggt tacagccgat ggcacagtcg gaggtgtgtt
    11941 cccaaccaac ccaacaacca actagcagca accaagtcat caggaggtga cttatgaaag
    12001 aacgagtagt tttcgaagcc aatgtaccgg tgacggcaac cttggcttat gcggatggat
    12061 tgaaagtgca aggacgcttc ggcgatcagg ttatgtactc gctgacggac gggcgagtca
    12121 tgtacgtgcc gccgatggtc cgggacaaac tggtcgagct tggtgtccgg cagaatgagc
    12181 cgtttgcgat ctgccgggcc gagcgtcgcg aaggcaaccg acggttcgtc gagtggattg
    12241 tgcaggctga cgggtcggca gcaccggctc agagtacgcc tgcgcacagt gatacacccg
    12301 gctcgccggt gaacggtgac agtggtgcgc gtggcaaagc gaatggtaac ggtaacggca
    12361 acggaaatgg caagctggcc agcgcatccc ccagttcagg gattgcagaa gcagcgttgc
    12421 gcgccgcact cacggcaagc attgacgcag ctcttgcagc agaacaatac gcgtccgcgc
    12481 acggtctgtc gatccgcttt ggcagcgaag atctgcgcgc catggcgctg agcctgttca
    12541 tccagcacgc ccgcgacgga ggtgtgcgat gaccgacgtt gccgtaaccg aaacgctggg
    12601 ggaggcgctc tcccccagcc aggtaaacac ctacatgaca tgtcccgcga aatggtactt
    12661 ccgctacttg atcggtttga gcgagccgac cacgggggca ctggcacttg gcaaagcttt
    12721 tcatggaaca ctcgcttggt acttccgcca gaagctgagc acaggtcgcg acatgaaagc
    12781 cgaagaactg cgcgaagtgt ttgt
//
LOCUS       DASRKK010000604        15007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1031804, whole genome shotgun sequence.
ACCESSION   DASRKK010000604 DASRKK010000000
VERSION     DASRKK010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031804"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1562
                     /locus_tag="VFC15_14440"
     CDS             195..1562
                     /locus_tag="VFC15_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZM11402.1"
                     /translation="MPKKKRPNSGNAPNAAASVKKPVPKSPQPRAAVKKSAPPTPVQP
                     PTATDPSLRSYSIPDYRLERPKFRVYQSGEGSNIEYKIEGDLSAAAPPIRESKYLSRE
                     QSIAIYRYMLLNRRMETVLENLYKQSLVVGGVYFGLGQEGCSCASAFALRDDDWLGPM
                     IRNQGAVLVRGFAPRDVMRQYMAKADSPTFGRDASSHFGDWEHRHVCAPISMLGELIG
                     VMAGVCLGARLQGKNIAGLTYIGDGGQSTGVTYESLNFAAVQKLGLVLIVENNLWGYS
                     TPSDMQFCVKDLAERAIAYGIPGVIVDGTDPCQVYDATYEACERARRGEGPTLIEAKM
                     MRMKGHAIHDAAHYVPAKLFEYWHARDPIARFENYLIKTKAWLSADENHKLIETVERE
                     LETERDAAVASPMPDPETDNTHQGVYCDASCHEIKPLYGPVKTAARKSTSTPAKSGES
                     AVHLK"
     gene            1648..2523
                     /locus_tag="VFC15_14445"
     CDS             1648..2523
                     /locus_tag="VFC15_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11403.1"
                     /translation="MKIFCTSVLILALTSVAFADFPFKKKEQEFKIHVPADVANTLRS
                     GTPVQRNDLALELGIFAPNPSSAAAKSSAPCVDFHHVEERPVTLHAGSENVVLLADSS
                     ECDSTYIVVFDKAPKSEWRHVQTVRLPSRAQRPEVTFAELIQPKVSEIVVHHETSRDS
                     GSVQQENFVVLKMQHDRVEVVLDTTERSEITLANRSVAETDNLQQTQTSTFELLKAAP
                     NSAAVFRILEKQVLTDNKTTITRYLVWTWNPELERFRSSPIDGGDVKPAPPPAKKPAT
                     KTPAEKPVPQPTKPK"
     gene            2574..3548
                     /locus_tag="VFC15_14450"
     CDS             2574..3548
                     /locus_tag="VFC15_14450"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZM11404.1"
                     /translation="MAPVTYLEAIRQGIWEEMERDPAVFCIGEDIGVYGGAFKVTEGL
                     IDRFGPARVVDTPISEMAIVNASFGAALTGMRPVAEFQFMDFISCAFNQITNLLAKAH
                     FRWGAPVPVVLRGPSGGGVHGGPFHSQNPETWFAHTPGLKVVCPSSAYDAKGLIKSAI
                     RDNNPVLFFEHKFLYRRIKEELPAEDYTVPIGKARVVREGRDVSVITYAAMVYVALEA
                     AEILAKVGIELEIVDLRTVLPLDRDAIRETVDKTNRVIVLHEDTKTGGIAGEVAAIIN
                     EECFDALDAPIVRIASADTPVPFSPPLEAAFLPKVEDVVREARRLKRY"
     gene            3715..4218
                     /locus_tag="VFC15_14455"
     CDS             3715..4218
                     /locus_tag="VFC15_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM11405.1"
                     /translation="MRGFLLGIIVTLVVVFGGAYWFVFTGHMDTRALPNGPGSFEKRT
                     AMHSLDEWVDAHAPKQDNPFKPTTDNIMDGSMVYDKHCALCHGSLKEPQSPLKNKFYP
                     PVPQLMNHTPDDPDANLFYVTKYGVKLTAMPGWDGVLSDDDIWKAVLFIKNSVQMKDT
                     STQPPAK"
     gene            complement(4292..4972)
                     /locus_tag="VFC15_14460"
     CDS             complement(4292..4972)
                     /locus_tag="VFC15_14460"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZM11406.1"
                     /translation="MKKNIAAAALLVLIATLAAHAQNLESVLNGMDRAAADFRTAQTD
                     FVWDQYEKVVDEHDLQKGTMYFRRQGDDVQMAADITVPDKKYVLFSGGMVSVYVPKAG
                     QITEYNAGKNKADFETFLVLGFGGRGHDLAKSFDVKYGGMEQVLGVNAAKLELTPKSQ
                     KVANMFQTITLWIDPARGVSVQQLFTEKSGDSRLAKYSNIQLNQKLSNDVFKLKTSGK
                     VTYVRPNG"
     gene            complement(4999..5367)
                     /locus_tag="VFC15_14465"
     CDS             complement(4999..5367)
                     /locus_tag="VFC15_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11407.1"
                     /translation="MGKISQTIKGYIWWMYPRGSVHYDVMVTLILAFIFLAPLWINFR
                     DKPTVRSPNQAEVVVKPEASGFLYTVDAAAIKPGADEEIREALLRVIEPVAGEISIDR
                     YEAVRDNKHNIVAYKVWGHR"
     gene            complement(5424..7916)
                     /locus_tag="VFC15_14470"
     CDS             complement(5424..7916)
                     /locus_tag="VFC15_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZM11408.1"
                     /translation="MSAPNPIRHTSDRVLEFDHLRELLAVYAASPLGQKRIASLAPSR
                     DRQWIEGQQLLTEELRGYVRSGGRFDFHGMLDPTPLLNKSRIQGAALELTEIRDVLLL
                     ADRAAEWREIVLHPPVAIEAKWDAVRELSQNVADFTELLRHFRNKILPDGTLDDRASP
                     ELSRIRRDIDRQRHTIQQSLQSYLRRLADGGAVQDELITIRGERFVIPVKVEQRRRVQ
                     GVAHGASSSGQTVFMEPLETIEQNNELVRLHDEELGEIHRILLEMTARIGEQAEALTE
                     AVEILGELELQFAKARFAKDYDCVAVKFLGGEAPQVTSASSAEVAQDENGDAFVLRNA
                     RHPLLERTLKPRGVAVVPLTVEIDSQHHQVIISGPNTGGKTVALKTIGLMALMAQSGI
                     PLPAERAELPIFDGVFADIGDYQSIEQNLSTFSAHVTNIDFISHHATRDSLVLLDELG
                     SATDPEEGAALAVSIADFFRRAGCLSIVSTHHTALKVYAANAEGVLNAAVGFDERTLA
                     PTFELRIGVPGASAGINIAQQLGLNPQIVAEAREKLTTQTHDVSRFLDRLHEQLREID
                     TERLALRAREQEIARERSHLAVEGKKEQREKIREMEKKLESVLHNFEYQARETVNAVQ
                     DRAQALKLSKDAERRISKMRREFREQFDATVVAHATGSDTGDKHATPHVVQHVSVGDT
                     VRLRSMGRDAKVLRHFDDDSFEVAVGSMKMKVPRDDIASTVMSAGANPVASARSRGIG
                     VSLSREDDSVPTEINVIGQTVDEATRAVEKFVDHAFLAGAVRVRIVHGSGMGILRKAL
                     RQFLQEHPHVASVAEPPQNEGGGGATVVELRT"
     gene            8136..9503
                     /gene="zwf"
                     /locus_tag="VFC15_14475"
     CDS             8136..9503
                     /gene="zwf"
                     /locus_tag="VFC15_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZM11409.1"
                     /translation="MQTNLSDAFVFFGATGDLAHKKIFPALQNMVKQGALNVPVIGVA
                     KSGWTIDQLRDRARDGITKFGGGVDEDAFKKLCELMVYIDGDYKDDKTYTQLRTQLGD
                     AQAPTHYLAIPPSMFPVVVNGLAQSGSSKNARVILEKPFGRDLLSARKLNETLHLAFP
                     EERIFRIDHYLGKEAVENLIIFRFANSFLEPIWNRNYVHSVQITMAESFGVEGRGKFY
                     EEAGAIRDVVQNHMLQVVAFLAMEPPTTMYTDSVRDEQVKVFRTIPPLSPAHIVRGQF
                     RGYRNEPGVSPNSDVETFAAIRLEVDSWRWSGVPFLIRAGKRMPTTATEVFVKLRKPP
                     LAKSGVDGRNYYRFRLGPDFELSLGARVKRPGPLMVPMPVELSAVKYTTTDEMDAYDR
                     LLTDAMRGDQLLFVRQDAVEAAWAIVDPILGDCCPTEMYEPGTWGPPDARELAAEVGG
                     WHDPE"
     gene            complement(9508..10236)
                     /locus_tag="VFC15_14480"
     CDS             complement(9508..10236)
                     /locus_tag="VFC15_14480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZM11410.1"
                     /translation="MATGTRRWNSEPNRMSDESRSVSPRAASALTAKTLLSAPPLTRE
                     FFIRDATTVGRELLGKLLIRREGRRLLAGRIVEAEAYLGAVDPASHAYIGRTPRNAVL
                     FGPPGHAYVYFIYGTYYCANVSCMPEGEAEGVLLRAMEPVWGLEAMAEARGLELTRES
                     PESQLRLVSSGPGRMSEALGITRARDNSKDLTSRKSDLWFADDGYRPERIAATPRIGI
                     KMAVEEPLRFVIVGNPYVSARRVR"
     gene            10321..11427
                     /locus_tag="VFC15_14485"
     CDS             10321..11427
                     /locus_tag="VFC15_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM11411.1"
                     /translation="MNKMIFSNLVHRPFRTFISVVAVAVEVTLILLIVGLSIGMLTDT
                     KNRQVGIGADIMVQPPGSSLIMGVSGAPVSIKVADKLRKLEHVVAVAPVVTQLSTAGN
                     LEVIDGIDLHTFEAMGGPFHYLSGGPFAGPNDMLIDDYVASAKKLKVGDTVETLNHPF
                     RISGIVEHGKGARKFLPIATLQDITGGQGKASIFYVKLDDPTNADAVVHEIKSIPGME
                     QYGVHSLKEYLSLMSSSSMPGLSTFIHVVIGISMIIGFIVIFQAMYAAVMERTREIGI
                     LKSLGAGKLYIVNVILRETVLIAIGGIVLGIAISFLAKAALHAKFQTLPILITPEWWM
                     VRAPIIAIVGALLGAAYPAMKAAQKDPIDALAYE"
     gene            complement(11523..12680)
                     /gene="dnaN"
                     /locus_tag="VFC15_14490"
     CDS             complement(11523..12680)
                     /gene="dnaN"
                     /locus_tag="VFC15_14490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZM11412.1"
                     /translation="MAAEATGAIQGTAMEISVSKFELLKELTATQGVVERKTTIPILS
                     NFLFEAANDRLTITATDLDLSLRTSCAAKVKREGACTIPARKLYDYVKLLPDGDISIK
                     LLENHWINIRSGRSNTKMVGMARANFPGLPVFPTSGSVKIPAQTLRSMIGRTIFAISS
                     EESRYTLNGGLIVLKPESITMVATDGHRLAHIEKTGEQFDVTGENKTLIPKKAMSELY
                     SLLQNSDVEQIEFAKDDSTLFFRIGERLLTSRQLTGQFPNYEAVLPRETTKSVVVHGA
                     DLNTAIQRVAQFADERSRAIRMKLDKNELKISSSSTESGESEDSIEIVYAADAMTIGF
                     NSEYLIDFLKASTGGEVRLEFKDAQSAGQMRPEDGSDDGFKYRYVVMPMRI"
     gene            complement(13032..14438)
                     /gene="dnaA"
                     /locus_tag="VFC15_14495"
     CDS             complement(13032..14438)
                     /gene="dnaA"
                     /locus_tag="VFC15_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZM11413.1"
                     /translation="MSYFGTASPNPWVLVLDALEKKVNRHSYETWLKPTRFSHSQGPT
                     LFVKVPNPDFCHIGEKYGDLISEAIENLGLEYQDVEFVTDPEKRAEKNDRRSGDGRSS
                     EAAVEAAAPVDNNGAAPVRQQRFDWDGAAQLNPKYTFDGFVIGSGNQFAHAASRAVAE
                     RPSKAYNPLFLYGGVGMGKTHLMQAIGHEIKKRNPQMAICYVSSEKFTNEMINALRNN
                     KMTSFRDKYRNMDVLLVDDIQFLVSKERTQEEFFHTFNALHESMRQIVIASDRPPKEL
                     AEIEDRLRSRFEWGLIADIQPPDLETKVAILQKKAESEQVLLPTDVALYIASNIRSNV
                     RELEGALIRLTAYVSLTGGDLTIQTAQEVLKNLIESQMRKITIESIQKAVSEQFGLRP
                     GEIKTKNNSHSIVYPRQIAMYLAKQLTEASLPEIGRQFGGKHHTTVLHSVDKIAEARK
                     TDKDLNRLLNKLTEVLGG"
     gene            complement(14768..>15007)
                     /locus_tag="VFC15_14500"
     CDS             complement(14768..>15007)
                     /locus_tag="VFC15_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11414.1"
                     /translation="PWKSLRDCHISTAPATARLVQYKIKKGAFLSHILRLPPGSFFNW
                     KRLLMSGGGARERRGGSRAGALINFQIVPIMVQKI"
BASE COUNT         3060 a         4650 c         4107 g         3190 t
ORIGIN      
        1 atacgctcag cctcccgtct gctgcattct caatcacttt cacccgttgg aactctttct
       61 gactcatcat caccatcctt cgagggtgac aaagtctcgt tgcagttagg gggtgacatt
      121 atcttggagc atctacagca aatcgctcta ggcttgcctc atctcctcca gacgtttatc
      181 gtttccctgc acgtatgccg aagaagaagc gtccaaatag cggcaatgcc ccgaacgcgg
      241 ccgccagcgt caagaaacca gtaccgaaat cgccgcagcc gcgcgctgcc gtgaagaaat
      301 cagcaccgcc tacgcccgtg cagccgccca cggcgaccga tccgtcgctt cgcagctact
      361 ccatcccgga ctaccgtctc gagcgtccga agttccgcgt ctaccagtcc ggcgaaggct
      421 ccaacattga gtacaagatc gaaggcgacc tgagcgccgc cgcgcccccg attcgcgaat
      481 cgaaatatct ttcgcgcgag caatccatcg ccatctatcg ctacatgttg ctcaaccgcc
      541 gcatggagac cgtcctcgaa aacctataca aacagagcct ggtcgtgggt ggcgtttact
      601 tcggactggg tcaggaaggc tgctcctgcg cctcggcttt tgccctgcgc gacgacgact
      661 ggctgggtcc catgattcgc aaccagggag cggtgctggt gcgcggcttc gcccctcgcg
      721 acgtcatgcg gcagtacatg gccaaagccg actcgcctac ttttggacgc gacgcttcat
      781 cgcatttcgg tgactgggag caccgccatg tttgcgcccc tatctcgatg ctcggagaat
      841 tgattggcgt catggctggg gtctgcctgg gcgcgcgctt gcagggcaag aacatcgcgg
      901 ggctcaccta tatcggcgac ggaggtcagt ccacgggcgt cacttacgag agcctcaact
      961 tcgccgccgt gcaaaagctt ggcctggtgc tgatcgtcga gaataatctc tggggatact
     1021 ccacgcccag cgacatgcaa ttctgcgtga aggacctggc cgagcgcgcc atcgcctacg
     1081 ggatccccgg cgtgatcgtc gatggcaccg atccgtgcca ggtttatgac gccacctatg
     1141 aggcctgcga acgcgctcgt cgtggcgaag gcccgaccct catcgaagcc aagatgatgc
     1201 gcatgaaggg gcacgccatt cacgacgccg cgcattacgt cccggcgaag ttgttcgagt
     1261 attggcacgc ccgtgacccc atcgcgcgat tcgaaaatta cctgatcaag acgaaggcct
     1321 ggctctctgc ggacgagaac cacaagctta ttgaaacagt cgaacgcgaa ctagagaccg
     1381 agcgtgacgc ggcagtcgcc tcacccatgc ccgatcccga gaccgacaac acgcaccagg
     1441 gcgtgtactg cgacgcttca tgccacgaga tcaagcctct gtacggacca gttaagacag
     1501 cggcacgcaa atccacatca acacctgcta agtcgggcga gtcagcggtg catttaaaat
     1561 gagtggaccg tcgcaaccca tgctcgccac cgcacacctc gacagccaac aagtggaacc
     1621 tcgctcgcat gggtttatgc attcggcatg aagattttct gcacgtccgt cctcatactg
     1681 gctctaactt ccgtggcgtt tgccgatttt ccgttcaaga agaaagagca ggaattcaag
     1741 atccacgtgc ctgccgacgt ggccaacacg ctccgcagcg gcacgcccgt ccaacgcaat
     1801 gacctggcct tggaattagg aatcttcgcc cccaacccat ccagcgcggc ggccaagtcg
     1861 agtgctcctt gcgtggactt ccaccatgta gaggagcgtc cggtgacgct gcacgctggg
     1921 tcggagaatg tggttctgct ggccgattcc agcgagtgcg attccaccta catcgtggtg
     1981 ttcgacaagg cgcccaagtc ggagtggcgg catgttcaaa ccgttcgcct tccgtcgcgc
     2041 gcgcaacgtc ccgaggtaac cttcgccgag ttgattcagc ctaaggtctc tgaaattgtg
     2101 gtgcaccacg agaccagtcg cgacagtgga agtgtccaac aggagaactt tgtagttttg
     2161 aagatgcagc acgaccgggt cgaagtggtc ctcgatacca ccgagcgcag cgaaatcacg
     2221 ctggccaatc ggtcggtcgc cgagactgac aatctgcagc agacccagac ttcgacgttc
     2281 gaacttctga aagctgctcc caactccgca gccgtctttc gcatactcga gaagcaagtc
     2341 ctgaccgaca acaagaccac catcacgcgc tacctggtct ggacctggaa cccggaactc
     2401 gagcgctttc gctcgtctcc catcgacggt ggcgacgtga agcccgcgcc gcctcctgcg
     2461 aaaaagccag caacgaaaac tcctgccgag aagcctgtgc ctcagcccac gaaaccgaaa
     2521 taacggggat tgcggataca atcgtcaatc ggagcggcat cggacagtcg aatatggcac
     2581 cagtcaccta cctcgaagcg atccgccagg gcatctggga agagatggaa cgcgatccgg
     2641 ccgtcttttg cattggcgag gacatcggcg tttatggcgg ggccttcaag gttaccgaag
     2701 gcctgatcga tcgcttcgga ccagcgcgcg tcgtcgacac acctatctcc gagatggcca
     2761 tcgtcaacgc aagctttggc gcggcgctca ccggcatgcg tccggtggcc gagtttcagt
     2821 tcatggactt catcagttgc gccttcaacc agatcaccaa cctgctggcg aaagcacact
     2881 tccgctgggg agcgcctgtg ccagttgtgt tgcgtgggcc atcgggcggt ggcgttcacg
     2941 gcggtccatt ccactcgcag aatccggaaa cttggtttgc ccacacgccg ggcctcaaag
     3001 tcgtctgtcc gtccagtgct tatgacgcca agggattgat caagtccgcc atccgcgaca
     3061 acaatcccgt gctatttttc gagcataaat ttctgtaccg ccgcatcaag gaagagctgc
     3121 ccgcagagga ttacacggtc ccgattggca aagcgcgcgt ggtgcgagaa ggccgcgacg
     3181 tcagcgtgat cacctacgcc gccatggttt acgttgcctt ggaggccgcg gaaattctgg
     3241 cgaaagtagg cattgagctg gagatcgtcg atcttcgcac agtgctaccg ctcgatcgcg
     3301 atgctatccg cgaaacggtc gacaagacca accgcgttat cgtgctgcat gaagacacca
     3361 aaaccggagg cattgccggc gaggtagcgg ccatcatcaa cgaagaatgc ttcgacgcgc
     3421 ttgatgctcc gatcgtgcgc atcgcatccg ctgacacgcc ggtcccgttt tcgccgccgc
     3481 tggaagcagc tttcttgccc aaggtcgagg acgtagtacg cgaagcccgc cgactaaagc
     3541 ggtactagag caattgcagc gcttctaagc tcctcctggt taggtcgaga acgcggcgcg
     3601 cgaagcccgc cgtttccaac ccaaatcgtc actcttctta acattttcgg ttgccgcgtg
     3661 atttaatctg gaagtctgag gcttcccaac gattctggag gtctaaggag agctatgcgc
     3721 ggattcttgc tcggtatcat cgtcactctt gtcgtggttt ttggtggggc ctactggttc
     3781 gtcttcaccg gacatatgga tacacgtgca cttcccaacg ggcccggcag cttcgagaag
     3841 cggactgcca tgcactccct ggacgaatgg gtggacgcgc acgctcccaa gcaggacaac
     3901 cccttcaagc cgacgaccga caacatcatg gatggatcca tggtgtacga caagcactgc
     3961 gccctctgcc acggcagctt gaaggagccg cagtcgccgc tgaagaacaa gttttatccc
     4021 ccggtgccgc agctcatgaa ccacactccc gacgatcctg acgcaaactt gttctacgtg
     4081 accaagtacg gagtgaagtt gacggcgatg cctggatggg acggagtact ttccgacgac
     4141 gacatctgga aggcggtgct attcatcaag aactcagtcc agatgaaaga cacatcaacg
     4201 cagccacccg cgaaatagag aattgccgaa ccagaaacgc ggaagccgcc agctggcggc
     4261 ttttgtactt gtagacttca cacgggcatc gctatccatt cggccgcacg taggttacct
     4321 tgccgctggt cttcagcttg aacacgtcgt tggacagctt ctggttcagt tgaatattgc
     4381 tgtacttcgc caggcgggag tcccccgact tctcggtaaa taactgttgc acgctgaccc
     4441 cacgcgccgg atcgatccac agcgtgatcg tctggaacat gttggcgacc ttttgcgatt
     4501 tcggagtcaa ctccagcttc gcggcattga cgcccagcac ttgttccata ccgccgtact
     4561 tcacgtcgaa cgacttcgcc aggtcgtggc cgcggccgcc aaatcccagc accagaaagg
     4621 tctcgaagtc agccttgttc ttgcccgcat tgtattccgt aatctgcccg gccttgggca
     4681 catacacgct gaccatgcct ccgctgaaca gcacatactt tttatccggt acggtgatgt
     4741 cggccgccat ctgcacgtcg tcgccctggc gacggaagta catggtgccc ttctgcaaat
     4801 cgtgctcgtc caccaccttt tcgtactggt cccagacgaa atcggtttgc gcggtgcgaa
     4861 agtcggcggc ggcccggtcc atgccattca gcacgctctc caggttctgg gcgtgcgcgg
     4921 ccagtgtcgc aatgagtacc agcaaagccg cagcagcaat gttctttttc atggtttatt
     4981 ccggcacaac cgccctgact agcggtgtcc ccacacttta taggcaacaa tattgtgctt
     5041 gttgtcacga acggcttcgt agcggtcgat gctgatttcg cctgcgaccg gttcgatcac
     5101 gcgtagcagc gcctcgcgta tctcctcatc ggcgcccggc tttatcgccg ccgcgtccac
     5161 tgtgtagagg aatccgctag cctcaggttt tacgaccact tcggcttgat tgggcgagcg
     5221 tacggtcggc ttatcgcgaa agttgatcca cagcggggca aggaaaataa aggcgagaat
     5281 cagcgtcacc atgacgtcat agtgcacgct accccgggga tacatccacc agatgtaacc
     5341 cttgatggtt tggctgatct tgcccatcgt cgtttcaagt ttagagagcc gcgcaaacaa
     5401 gtgcaagaaa cctggcccgc gcgctacgtc cgcaattcca ccacggtcgc gccgccgcca
     5461 ccttcattct gcggaggctc cgcgacgctc gccacgtgag gatgctcctg caggaactgc
     5521 cgcaacgcct tgcgcaatat ccccatgccg ctgccatgca cgatgcgcac ccgcacggct
     5581 cccgctagaa acgcgtgatc cacgaacttc tccaccgccc gggtggcttc atccacggtt
     5641 tgaccgatta cgttgatctc ggtgggaacg ctgtcgtcct cgcgggatag agacacaccg
     5701 atgccgcgcg atcgcgcgga tgccacagga ttggctccag cactcatcac cgtgctggca
     5761 atgtcgtcgc gtggaacttt catcttcatc gaccccacgg ccacttcgaa ggagtcgtcg
     5821 tcgaaatggc gcagcacttt ggcatcgcgt cccatcgacc gcaaccgcac cgtatctccc
     5881 acggaaacat gctgcaccac gtgcggtgtg gcatgtttgt cgccggtatc ggagccagtc
     5941 gcgtgcgcca caaccgttgc gtcgaattgc tcgcgaaatt cgcgacgcat tttggaaatc
     6001 cgccgctccg cgtctttcga cagcttcagc gcctgcgcgc ggtcctgcac cgcattgaca
     6061 gtctcgcgcg cctggtattc gaagttgtgc agcacgctct ccagtttctt ctccatctcc
     6121 cggatttttt cgcgctgctc tttcttacct tccaccgcga gatggctgcg ctcccgcgcg
     6181 atttcctgct cgcgagcgcg caacgccagc cgttccgtgt cgatttcgcg caactgctca
     6241 tgcaggcgat cgagaaaacg tgaaacgtcg tgcgtctgtg tagtcagttt ctcgcgcgct
     6301 tcagctacga tctgcggatt cagccccaat tgctgcgcga tgttgattcc cgccgatgca
     6361 ccgggtacgc cgatgcggag ttcaaacgta ggagccaggg tccgttcatc gaaacccacc
     6421 gctgcattaa gcacgccctc ggcattcgcc gcgtaaacct tcaaggccgt gtgatgggtg
     6481 gaaacgatgc ttaggcagcc tgcccgccgg aagaagtcgg caatcgatac tgccagtgcc
     6541 gcgccctcct ccggatcggt tgccgagccc aattcgtcca gcaggaccag cgagtcccgg
     6601 gtcgcgtgat gcgagatgaa gtcgatgttc gtgacatgtg ccgaaaatgt agaaagattt
     6661 tgttcgatcg actgatagtc gccgatgtcg gcaaacacgc cgtcaaaaat gggcaattcc
     6721 gcacgctctg caggcagcgg aatgcccgat tgcgccatca acgccatcag tccgatcgtc
     6781 ttgagcgcga ccgttttgcc gcccgtgttg ggcccgctga tgatgacctg gtgatgctgg
     6841 ctgtcgatct cgaccgtgag cggaacaaca gccacccctc tcggcttgag cgtgcgttct
     6901 aaaagcgggt gtcgggcatt gcgcaggaca aacgcatcgc cgttctcgtc ctgcgctact
     6961 tccgccgacg atgccgacgt gacttgcggc gcctctccac cgagaaattt aacggccaca
     7021 cagtcgtaat cctttgcgaa gcgcgccttg gcaaattgca attccaactc gcccagtatc
     7081 tcgacagcct ctgtcagggc ttccgcctgc tcgccgatcc gcgccgtcat ttccagcaga
     7141 atgcggtgga tctcgcccag ttcttcgtca tgcaggcgaa ccaattcgtt gttctgctcg
     7201 atggtctcca gcggctccat gaacactgtt tgcccgctgg acgaggcgcc atgcgccaca
     7261 ccctgcaccc gtcgtcgctg ttccaccttg accggaatca cgaagcgctc gccgcggatg
     7321 gtgatcagct cgtcctgcac ggccccgccg tccgccagcc ttcgcaaata cgactgcagc
     7381 gattgctgga tggtgtgccg ttggcggtcg atgtcgcggc gtattcgtga cagctccggc
     7441 gacgcccggt cgtccagcgt tccgtcgggc aaaatcttgt tgcgaaagtg acgcaacagt
     7501 tcggtgaagt cagcgacgtt ttgcgaaagc tcgcgcaccg catcccactt agcttcgatg
     7561 gccactggag gatgcagcac aatctcacgc cactccgccg cccggtccgc caacagcaaa
     7621 acatctcgga tttcggtaag ttccagcgcc gctccctgaa tgcgcgactt gttgagcagc
     7681 ggcgtgggat cgagcatccc gtggaagtcg aatcggccac cgctgcgcac gtatccgcgc
     7741 agctcctcgg taagtagctg ctggccttcg atccactggc gatcacgaga cggcgcaagg
     7801 ctggcgattc gtttctgccc cagtggggaa gcggcataca ccgccagcag ttcgcgcagg
     7861 tggtcaaatt ccaaaacgcg atcgctggtg tgccggatgg gattgggagc gctcatcgga
     7921 agaccaactt atggtacagg agcacatcga ccgcgcagta gttcctggtg ttcgccgcgc
     7981 cagtgcttga tttacagtct attcacgctc gtaggcgcac acggctaccc tacgttgcgt
     8041 cctgcgatta cactgttcgg gtaggacagc cccgtctagc ctgtttgccg taacggatca
     8101 tctgtgttga ttcgccatca caacaagagg aacgcatgca aaccaatctc tctgacgctt
     8161 ttgtattctt cggtgccact ggcgacctag ctcacaagaa aattttcccc gcgctgcaga
     8221 acatggtcaa gcaaggcgcc ttgaatgtgc ccgtcatcgg cgtcgccaaa tcaggatgga
     8281 cgattgacca gttgcgcgat cgcgcccgtg acggcatcac gaagttcggc ggaggcgtcg
     8341 atgaggacgc gttcaagaag ctctgcgagt tgatggttta tatcgacggc gactacaagg
     8401 acgataagac ctatacccag ctcaggacac agctcggcga cgcgcaggcc cctactcact
     8461 atctggcgat tccgcccagc atgttccccg tagtggtgaa cggtctggct caatccggtt
     8521 cctcaaaaaa tgcgcgggtc atcctggaaa aaccatttgg acgtgacctg ctctcggcgc
     8581 gcaagcttaa cgaaaccctg catctggcat tccccgaaga acgcatcttc cgcatcgacc
     8641 actacctcgg caaagaggcg gtggagaatc tcatcatctt tcgcttcgcc aatagctttc
     8701 tggaacccat ctggaaccgc aattacgtcc atagcgtgca aatcaccatg gctgagtcct
     8761 tcggcgtgga aggacgcggc aagttctacg aggaagctgg cgccatccgc gacgtagtgc
     8821 agaaccacat gctgcaggtc gtcgcgttcc tcgcaatgga gccgcccacc accatgtaca
     8881 ccgactcggt gcgcgacgag caggtcaagg tcttccggac cattccgccg ctcagccctg
     8941 cccacatcgt gcgcgggcag ttccgtggtt atcgcaacga gccgggcgtc agcccgaact
     9001 ccgacgtcga gacttttgcc gccattcgcc tggaggtgga ttcctggcgc tggtcaggtg
     9061 tgccgttcct gattcgagcc ggcaagcgca tgcccaccac cgcaacggaa gtcttcgtga
     9121 agttacgcaa acccccactc gccaagagcg gcgtggatgg acgcaactat taccgcttcc
     9181 ggctgggtcc cgacttcgag ttgagtctcg gtgcacgggt caagcggccc ggaccactga
     9241 tggtccccat gccggtcgaa ctctcggccg tcaaatacac caccaccgac gagatggacg
     9301 cttacgaccg gctgctcacc gatgccatgc gaggcgacca gttgctcttc gtgcgccagg
     9361 acgcggtgga agccgcttgg gcgatcgtcg atcccatcct tggtgactgc tgtcccacgg
     9421 aaatgtatga gccgggaacc tggggacccc cggatgcacg tgaactggcg gcggaagttg
     9481 gcggctggca cgatccggag tagcgcgtca gcgaacgcgc cgcgccgaga cgtagggatt
     9541 tcccacaatc acgaagcgca gcggctcctc gactgccatc ttgattccga tgcgaggagt
     9601 agctgcaatt cgctctggac ggtaaccatc gtcggcaaac cacaaatcag acttccggct
     9661 ggtcaagtct ttgctgttgt cccgagcgcg tgtgattccc agcgcttccg acattcttcc
     9721 cggaccgctg gaaaccagcc gcagttgcga ttcgggcgac tctctggtca attcgaggcc
     9781 tcgggcttcg gccatcgctt ccaagcccca caccggctcc atggcgcgta gcagaacgcc
     9841 ctctgcctca ccctcaggca tgcacgaaac gttggcgcag taataggtcc cgtagatgaa
     9901 atacacgtac gcgtgcccgg gtgggccgaa gagcacggca ttgcgcggcg tacgtcctat
     9961 ataggcgtgt gaggccggat cgacggcccc aagataggct tcggcttcca caatccgccc
    10021 ggccagtaat ctacggcctt cgcggcgaat cagtagcttg cccagcagtt cccgcccgac
    10081 tgttgtcgcg tcgcgaatga aaaactcgcg agtcagcgga ggagcggaca gtaatgtttt
    10141 cgccgtcagc gcagacgccg cccgtggcga cacggatcgc gattcatcgc tcatccggtt
    10201 cggctccgag ttccagcgac gggttccggt tgccatgctt tccattctag tttcgcagcg
    10261 attcgcgcgt attgcgggcc ggtcgtcccg ccccaagtcc tatacaatcg aaagtgaatc
    10321 atgaataaga tgatcttttc caacctggtg caccgtccct tccgcacctt catcagcgtg
    10381 gtcgcggttg cagttgaagt aaccctgatt ttattgattg tcggcctctc gattggcatg
    10441 ctcaccgaca ctaagaaccg ccaggtcggc atcggggccg acatcatggt gcagccgccg
    10501 ggctcttcgc tcatcatggg cgtcagcggt gctccggtgt ccatcaaggt tgccgacaag
    10561 cttcgcaaac tcgagcacgt cgtcgctgtt gcccccgtcg ttacccagct ttccaccgcc
    10621 ggcaatctgg aagtcattga cggcatcgat ctccacacct tcgaggccat gggtgggccg
    10681 ttccactacc tttcgggcgg accgttcgct ggccccaacg acatgctgat cgacgactac
    10741 gtggcctccg cgaagaagct gaaggtcggc gacacggtcg agacgctgaa tcatcccttt
    10801 cgcatttcag gcatcgtgga gcacggtaag ggagcgcgca aatttcttcc catcgccacc
    10861 ttgcaggaca tcaccggcgg gcagggcaag gcctcgatct tttacgtcaa gctcgacgat
    10921 cccaccaacg ccgacgccgt ggtgcacgag atcaaaagca ttcccggcat ggagcagtac
    10981 ggcgttcact cgctgaagga atacctaagt ctgatgagtt cgagcagcat gcccgggctt
    11041 tccactttca tccacgttgt catcgggatc tccatgatca tcggctttat cgtcatattc
    11101 caggcgatgt atgccgcggt gatggagcgc acccgcgaga tcggcatcct gaaatcgctg
    11161 ggcgctggca agctctatat cgtgaacgtc atcctgcgcg agaccgtact gattgccatc
    11221 ggaggcatcg tcctcggcat cgccatcagc ttcctcgcaa aagctgcact acacgccaag
    11281 tttcagacgc tgccaatcct gataacgccg gagtggtgga tggtgcgcgc cccgatcatc
    11341 gccatagtcg gcgcgctgct gggtgccgct tatcccgcaa tgaaggccgc ccagaaagac
    11401 cccatcgacg cgctggcgta cgaatagaag actcgttgcg acgcggttcg aggcgtggcg
    11461 ggagtatgaa aaagccggat ttggcatccg gctttcattc ttactaatgg ctccaacgta
    11521 cttcagatcc gcatcggcat cacgacgtaa cggtacttga atccgtcatc gctgccgtct
    11581 tcgggacgca tctgccctgc cgactgcgcg tctttgaact ccagacgcac ttcgcccccc
    11641 gtcgaggcct tcaagaagtc gatcaggtac tcggagttga agcctatcgt cattgcatcg
    11701 gccgcgtaaa caatctcaat cgagtcttcc gactcgccgg attccgtgga cgacgaggag
    11761 atcttcaact cattcttatc gagcttcatg cggatcgccc gcgagcgttc gtccgcgaac
    11821 tgggccacgc gttgtatcgc ggtattgaga tcggcaccgt gaacgacgac tgacttggtg
    11881 gtctcacgcg gcagcaccgc ttcgtagttg gggaactggc cggtcaactg acgcgacgtc
    11941 agcaaccgct ccccgatgcg gaagaatagc gtggagtcgt ccttggcaaa ttcaatctgc
    12001 tcgacatcgc tgttttgcag cagcgaatac aactcgctca tcgctttctt ggggatcagc
    12061 gtcttgttct cgcccgttac atcgaactgt tcgccagttt tctcgatgtg cgccaggcga
    12121 tgtccgtcgg tcgccaccat ggtgatcgac tccggcttca acacaattag tccgccgttt
    12181 agcgtgtaac gtgactcctc gctggagatt gcgaaaatgg tccggccgat catgctgcgc
    12241 aacgtctgcg ccggaatctt gacgcttccg gatgtcggga acaccggcag tcccggaaaa
    12301 ttcgctcgtg ccattccgac catcttggtg ttcgagcggc ccgagcgaat gttgatccag
    12361 tggttctcca gcaacttgat cgagatgtcg ccatcgggca gcaatttgac gtagtcatac
    12421 agcttgcgtg ccggaatcgt gcaggcgccc tcgcgcttca ccttggccgc gcacgacgtc
    12481 cgcagactca ggtccagatc ggtcgccgta atcgtcaacc ggtcattggc tgcttcaaat
    12541 aaaaaattgg acaggatggg aatcgtcgtc ttgcgttcca cgactccctg cgtggcagtc
    12601 agttccttga gcaactcgaa tttgctgacg ctgatttcca tcgcagttcc ctggattgcg
    12661 ccggttgctt ccgcagccat atcttccacc cttggagtcg cgcccttagc ccagcctttg
    12721 taccgtggcg tgtggcagga tgttgcgaga acccgcgact actgcatttc cttatatcaa
    12781 attaaaaacc tataaaacag tagtaacagt agacacaccg gaaaagtgga aaagaagtcc
    12841 aaaaccgcgt caatcaggct ggcagcccac atccccgcat accaaaactg ctgcgccgcc
    12901 cgtgagtgtc tctgccaaac cgaaccctcg taagaaatcc gaccacttct tccacgtgat
    12961 ccacaatccg tccacagccc ttgccgagga aaactgcgcc caatagtgaa aaaccgcgcc
    13021 ataagcgcgg ttcatccccc cagaacctcg gtgagtttgt tcaacagcct gttcaaatct
    13081 ttgtcagtct tgcgggcttc ggcgatcttg tccaccgaat gcagcaccgt ggtgtgatgt
    13141 ttgccgccga attgccgtcc gatttccggc agtgacgcct cggtcaactg cttcgccagg
    13201 tacatcgcga tctggcgcgg gtaaacgatc gagtgggagt tgttctttgt cttgatctcc
    13261 cccggccgca gcccaaactg ctccgacacc gccttctgga tcgactcgat ggtgatcttg
    13321 cgcatttgcg actcgatcag gttctttagc acttcctgcg cggtttgaat ggtcaggtca
    13381 ccaccggtca gcgacacata agcggtgagc cgaatcagcg ctccctccaa ctcgcgcaca
    13441 ttgctacgaa tgttcgacgc aatgtacaac gcaacgtccg tcggcaacag cacctgctcc
    13501 gactccgcct tcttctgcag gatagccacc ttggtctcaa gatcgggcgg ctggatgtcg
    13561 gcgatcagcc cccattcgaa gcggctgcgc aaccggtctt cgatctccgc caactctttc
    13621 ggcggacggt cgctggcaat cacaatctgc cgcattgact cgtgcagcgc gttaaaggta
    13681 tggaagaact cctcctgcgt gcgctccttg ctgaccagga actggatgtc gtccaccagc
    13741 agcacgtcca tgttgcggta cttatcgcgg aaactggtca tcttgttgtt ccgcaatgcg
    13801 ttgatcatct cgttggtgaa cttttcgctg gaaacatagc agatcgccat ctgcggattc
    13861 cgcttcttga tctcgtgtcc aatggcctgc atcaggtgcg tcttgcccat accgacgcca
    13921 ccgtacagga agagtgggtt gtaggcctta gaaggccgct ccgccaccgc gcgcgaggcc
    13981 gcatgcgcga actggttacc cgatccgatc acgaatccat cgaacgtgta cttgggattc
    14041 agttgcgccg cgccgtccca gtcaaacctc tgctgccgga ccggtgccgc accgttatta
    14101 tcgactggcg ccgccgcttc cacggcagcc tcactgctgc gtccgtcgcc gctgcggcgg
    14161 tcgttctttt cagcgcgctt ctctggatcg gtaacgaact cgacgtcctg gtattccagt
    14221 cctaggttct caatcgcctc gctgatcagg tccccgtact tctcgccgat gtggcagaag
    14281 tccgggttcg ggaccttcac gaacagtgtc ggtccctggg aatggctgaa gcgggtcggt
    14341 ttgagccagg tctcgtacga atggcggttg accttctttt ccagcgcatc gagtacgagc
    14401 acccatggat ttggcgaggc agttccaaaa taagacatag caaaattaac ccggaggcaa
    14461 gccttgcctc gaatcgcttc aattagaatt cagggggacg actgccagta aagccacgat
    14521 ccaccgcacg attcaggaga atgtctaaga tccgtaaccg aagaggctaa ggtacaactg
    14581 gcattccgtg agcacatgcg cagaatgtct tctctgtagc tggatgggat actagcacga
    14641 aaaattcaca gcacaagata ttggttgcac tcgaagcgcg cgattgcttt gcgagtgatt
    14701 gattttgtga atgttactgg cagaaaaatt ttttactgca gcgcccgtgg tgcaaattgc
    14761 aactccatca aattttctgc accataattg ggacgatttg gaaattgatc agcgcgccag
    14821 cgcgactccc accgcgccgt tcgcgggccc ctccgccgga catcagtagt cttttccaat
    14881 tgaagaatga gcctggagga agcctgagga tatggctgag gaaagctcct ttcttgattt
    14941 tgtattgaac gagtctagca gtcgccgggg cggtggaaat gtggcaatcg cgaagcgatt
    15001 tccaagg
//
LOCUS       DASRKK010000605         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_347415, whole genome shotgun sequence.
ACCESSION   DASRKK010000605 DASRKK010000000
VERSION     DASRKK010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347415"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1180
                     /locus_tag="VFC15_14505"
     CDS             38..1180
                     /locus_tag="VFC15_14505"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11415.1"
                     /translation="MKKVRYGALVMQHRFGSSLNLNTHLHAVFPDGVFSRHPSRKVKF
                     HELRPPEPDDLEDIAFNVHQRFLRWLQRHGLLKNEAESGFSNESPELTALQACAQGSL
                     GLGNLVKKRSKPRTSNQDADEGGFEQRAVNGRVGVSHGYSLYAGNPISAWDRDGRERL
                     LRYCLRAPLSLERLSIGPDGNVIYQVKATRHGNETRRVMTPMQFMARLVALIPPPYHP
                     LLRYFGVFGPHASWRKAVVPDVAPATEHQHDLAKPPAAWTLKTDPSHSASTTSPKSPA
                     DPSVASTILAAPARESKQETRNQDIGPRLGAPWRIDWAALLRRVHEIDSLACPCGGRL
                     KFVELVTEPTTAREVLASMGLPTDLPPIAKARSPDFYQESFPADWD"
     gene            complement(1351..2664)
                     /locus_tag="VFC15_14510"
     CDS             complement(1351..2664)
                     /locus_tag="VFC15_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HZM11416.1"
                     /translation="MSAPVLTMSKQEFERAVLLRKVLDKRLTQRKAAEILGLSLRQVE
                     RLCRRYRTAGPAALASRRRGRVSNHRAAASHRQSVLGLVRARYADFGPTLAREKLREC
                     HGLEVSKETLRKWMTEDGLWVPHARRRDRVQQPRNRRTCLGELIQIDGCDHEWFEERA
                     ARCTLLVYVDDATSRLMQLHFCDSESTFTYFEATRGYLDSHGKPVAFYSDKYSVFRVN
                     QPEPRGGDGVTQFGRALNELNIDIICANSPAAKGRVERANLTLQDRLVKELRLQGISS
                     IAEANAFAPTFLADYNARFAKAPSNAFDAHRPMLPSEKLDDIFTWQETRRVSRSLSFN
                     YQRKLFLLQETDVTRALAGQRVTVFELDDGTVQVRHGSKVLPATQFGREDADITQGAI
                     VSNKLLAGVLQHIKDKQTKKDSDKFDKLRTHREKRLLLQRAKAVA"
BASE COUNT          615 a          849 c          864 g          631 t
ORIGIN      
        1 gtttcgctgg cagcgcgaac gggcgcgaga ggaagagttg aagaaggttc gttacggagc
       61 gctggtgatg cagcatcgtt ttgggtcgtc tttaaatctt aacacgcacc tccacgcagt
      121 ttttccagat ggcgtgttct cgcgacatcc ctccagaaag gtcaaatttc atgagcttcg
      181 cccacccgag ccagatgatc tcgaggatat tgccttcaac gtccatcagc gatttttgcg
      241 ctggctacag cggcacgggc tgctcaagaa cgaggccgaa agcggcttct cgaatgagtc
      301 ccccgagctt acggccctac aagcctgcgc tcagggctcg ctcggactcg gcaatttggt
      361 gaagaagcgc agcaagccac gaactagtaa ccaggatgcc gacgagggtg gattcgagca
      421 acgagcggtc aatggtcgtg ttggcgtgag ccacggctac agcctgtacg ctggcaaccc
      481 gatatccgct tgggacagag acggccgaga gcgactgttg cgctactgcc tgcgtgcgcc
      541 actcagcctt gagcgcctgt ctattggacc tgacggcaat gtgatctacc aagtcaaagc
      601 gactcggcac ggcaacgaga cacgacgagt aatgaccccg atgcagttca tggctcgatt
      661 ggtcgcgtta attcctcccc cgtatcatcc attgttgcgt tacttcggcg tatttggacc
      721 gcacgcatcg tggcgaaaag cggtagtgcc cgacgttgca ccagctaccg agcatcagca
      781 cgatcttgca aagcccccgg cagcatggac tttgaaaaca gatcccagtc attccgcgtc
      841 aactacttcg ccaaagagtc cagcagatcc atccgtcgca tcgacgatac tggcagcacc
      901 agcaagggag tcgaagcaag agactcgcaa ccaagatatt ggacctcgcc tcggcgcgcc
      961 gtggcgtata gattgggctg ccttgctcag gagagtgcat gaaattgatt ctctggcctg
     1021 cccttgtggc ggcaggttga agttcgtcga actcgtcacc gaaccgacca cggcacggga
     1081 agtcctggca agcatgggat tgccaaccga cctgccgccc atcgctaaag cacggtcacc
     1141 ggacttctac caagagtctt tccccgccga ctgggactga actccagtct gggccacacg
     1201 cgcgctggtc accgaatcgc ccgaccaagg gcgatggtgc gctttggatt cgatcaaggc
     1261 tgctcaaact tccgctttag gtgcacgcac ttgtcaaggc aaagtagaaa tgtcgggatt
     1321 ctggcaacat agagatgtcg ggttaggggg ctaggcgact gccttggctc gttgcagaag
     1381 caaccgtttt tcgcggtggg ttcgtagctt atcgaatttg tcggagtcct tcttggtctg
     1441 cttgtctttg atgtgttgaa ggactccagc aaggagcttg ttggagacaa tggcgccctg
     1501 ggtaatgtcc gcatcttccc taccgaattg agtcgccggc aggaccttgc taccgtgccg
     1561 cacttggacg gttccgtcgt cgagttcgaa gacagtcacg cgttggccag ccagagcgcg
     1621 agtcacatcc gtttcttgca gtaggaagag cttgcgctgg taattgaagc tcagcgatcg
     1681 gctgacacgg cgcgtctctt gccacgtgaa gatgtcatct agcttttcac tgggtagcat
     1741 aggacgatgt gcgtcaaacg cattgcttgg ggctttggcg aagcgtgcgt tgtagtcggc
     1801 cagaaacgtt ggggcgaacg cgtttgcttc ggcaatgctc gaaataccct ggagcctgag
     1861 ctctttgacc agtcggtctt gcagggtcaa gttggcgcgc tcgacccgtc ctttggcggc
     1921 aggtgagttg gcgcaaatga tgtcaatgtt gagctcattc agcgctcgac cgaattgcgt
     1981 gacaccatca ccacctcgtg gctcgggctg attgacgcgg aacacggagt acttgtcact
     2041 gtagaaggcg acgggcttac catgactatc gagataccca cgggtggctt caaagtaagt
     2101 gaaggtcgac tccgagtcgc agaagtgaag ctgcatgagc cgactggtgg catcatccac
     2161 gtagacaagc aacgtacaac gagcagcacg ctcttcgaac cactcgtggt cacagccatc
     2221 gatctgaatg agctcaccca ggcaggtcct gcgattgcga ggctgctgaa cacggtcgcg
     2281 acggcgtgca tgcggcaccc acagcccgtc ctcggtcatc cacttgcgca gcgtctcttt
     2341 ggaaacctcc aggccgtggc attcccgaag cttctctcgg gccaaggtcg gcccgaagtc
     2401 agcgtagcgg gctctgacga gcccgaggac gctctgcctg tgcgacgccg ccgcgcgatg
     2461 gttgctcact cggccccgcc gccgtgaagc caaagccgca ggcccagctg tgcggtagcg
     2521 acggcaaagg cgctcaactt gccgcaagct gaggccgaga atctcggccg ctttgcgttg
     2581 agtaagacgc ttatccagga ctttgcgcag caaaaccgcg cgctcgaatt cttgcttgct
     2641 catggtcagg accggggccg acatgcccgg tcacgtaccg cgaacccgac atttctactt
     2701 tgcccaaacc cgacatttgc actttgctcc tacagcactc ctgcgcatac gtgggcgtta
     2761 tgtccacttc cgcgtgtctc tgtggcgtgg gtcggcggga tgatggcctt tgcgatacct
     2821 agacccagat tgacatcatg gagtactaca acggaaagat tttggccaac gtagcttgcg
     2881 gaactagcac gcgttggtct gccaagtggg actcggcgac tcgcgcggtc aatgcaagtt
     2941 gggctgcgtc ctatcacgt
//
LOCUS       DASRKK010000606         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_739171, whole genome shotgun sequence.
ACCESSION   DASRKK010000606 DASRKK010000000
VERSION     DASRKK010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739171"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFC15_14515"
     CDS             <1..936
                     /locus_tag="VFC15_14515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZM11417.1"
                     /translation="PLPSWNAGPAKQAILNFVKVTTGPASPDFVPPGQRIATFDQDGT
                     TWVEHPMYTQFVFAFERVAELAPQHPEWKTTQPFQTVLSGDKAALTKLTMKDVATIMI
                     ATHTGMTVDQFHDIAKDWLAKARDKRWDRPYTGLTYQPMLEAMQYLRANGYKTYIVTG
                     GGQDFVRVYAEQVYGIPPEQVIGSALETQYTYSKQGEGVLMRPPKLLLNDDFSGKPED
                     IYLFTGRHPKAAFGNSTGDQQMLEYTQAGGGAPLMMIVLHDDAQREYAYGPAQGLPDS
                     KVGTFTQALYDEAKSKGWFVISMKNDWKRIFAWEK"
     gene            1394..1759
                     /locus_tag="VFC15_14520"
     CDS             1394..1759
                     /locus_tag="VFC15_14520"
                     /inference="COORDINATES: protein motif:HMM:NF023170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HZM11418.1"
                     /translation="MGFLDVFSLLILLGLILGGVCLILLLAYTPGAIARKRRSPWAEA
                     VNIAGWVGILVFPIWILALAAAFIRPRTGGGAAIAISEAETAELAATISSISERLNNL
                     ENGLRTVLPKVVPSRAEGA"
     gene            1762..1980
                     /locus_tag="VFC15_14525"
     CDS             1762..1980
                     /locus_tag="VFC15_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11419.1"
                     /translation="MLVAILVIGYVLVLRYLDQKNREKDRLKAGLSVPSGPADYATVK
                     KALAETSERLAVVEGQYRELVQRLGVTP"
     gene            1977..>2221
                     /locus_tag="VFC15_14530"
     CDS             1977..>2221
                     /locus_tag="VFC15_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11420.1"
                     /translation="MIAFLAAVYCIGLYLIFIKFRWLPFNLLAQVFVGAVGFIGLLIV
                     LFGMNYTQPFSIGTTVSGVTTQIEARVPGKVIEVDVK"
BASE COUNT          487 a          625 c          648 g          461 t
ORIGIN      
        1 ccgctgccgt cgtggaatgc cgggccggcg aagcaggcga tccttaactt cgtgaaggtc
       61 acgaccggcc cagcgagtcc ggacttcgtc ccaccggggc agcgcatcgc caccttcgac
      121 caggatggca ccacgtgggt cgaacacccc atgtacaccc agttcgtatt tgcctttgag
      181 cgggtcgccg agttggcgcc gcagcacccg gaatggaaga ccacgcagcc atttcagacc
      241 gtgctttctg gcgacaaggc cgcgctgacg aagctcacca tgaaagacgt ggcgaccatc
      301 atgattgcca cccacaccgg catgacggta gatcagttcc atgacatcgc aaaagactgg
      361 ctggcgaagg cgagggacaa gcgttgggac cggccttata ccggcctcac ttaccagccc
      421 atgctggagg cgatgcagta tctccgcgcc aacggctaca agacctacat cgtcaccggc
      481 ggcgggcagg acttcgttcg cgtctatgcc gagcaagtgt atggtatccc gccggaacag
      541 gtcatcggct ctgccctcga gacgcaatat acctatagca agcaaggcga aggggtcttg
      601 atgcgtcccc cgaaactgct gctgaacgac gacttttccg gcaagccgga agacatttat
      661 ctctttaccg gccgccaccc caaggcggcg tttggcaact caaccggcga ccaacagatg
      721 ctggagtaca cccaggcggg cggcggcgca cccttgatga tgattgtgct tcacgatgac
      781 gcacagcgcg agtatgccta cgggccggcc caaggactgc ctgatagcaa ggttggcacc
      841 ttcactcagg ccctgtacga cgaagccaag tcgaagggtt ggttcgtcat cagcatgaag
      901 aacgactgga agcggatttt cgcttgggag aaatgatccg acaatagagg aggttggaat
      961 ggttgaacgg tccatttcac atgaacgttg gtaggaccca ctcatcgcga cccctcccga
     1021 agcggatgac cactggaaga tcgcaagggt cgttcaactc ttttttccac cgtggcggca
     1081 aacgaacgaa ggcaaccctc atcgccctca ggtgattgag aactcaacac cgttgcactc
     1141 aaaaggagaa atggaagagt gaagacaatc cgagagtgcg tcgtcgggcg tcgcaccggc
     1201 ccaaaacatc cgatggaaac ttcttttgtc tgtagggttc ggcgagtact atgtgatgcg
     1261 atctcgctcc gacgagataa attgtagaac tcaacgatag gactgaagag aagcgagcac
     1321 agagtttgcg gcaaaactgg gtacaaatca accggtagtc aaggtgtaac gagccttttt
     1381 tccggagcgt actatgggtt ttctcgatgt tttttccctg ctgatactgc ttggcctgat
     1441 tcttggggga gtgtgcttga tcttgctcct tgcctacact ccgggcgcca ttgccaggaa
     1501 acgccgcagt ccatgggccg aagctgtcaa catcgcagga tgggtcggca ttctggtgtt
     1561 tcccatctgg atactggcgc tggctgcagc attcattcgc ccgcgcaccg gcggaggcgc
     1621 cgcgatcgcg atcagcgaag ccgagactgc tgaattggct gcaaccattt cgtcgatttc
     1681 ggagcgcctc aacaatctgg agaacggatt gcgaacggtg cttccgaagg tagtgccctc
     1741 tcgcgcagaa ggagcatagc catgttagtg gcgatacttg ttatcggcta cgtgctggtc
     1801 ttgcgatacc tcgaccagaa gaaccgggag aaagaccgtc tgaaagctgg cctctctgtg
     1861 ccatcgggac cggcagacta cgcgaccgtc aagaaggctt tggcggaaac ctcggagcgg
     1921 ctagccgtgg ttgaagggca atatcgcgag ctggtgcagc gcctgggggt gactccgtga
     1981 tcgcctttct cgccgccgtc tattgcattg gcctctacct catcttcatc aagtttcgct
     2041 ggctgccgtt caatctgttg gcgcaggttt tcgtgggcgc cgtaggcttc attggtctgc
     2101 tgattgtcct gttcggtatg aactacaccc agccgttttc cattggcact accgtctcag
     2161 gcgtgacaac gcagatcgag gcgcgagtgc cgggcaaggt aatcgaagtg gacgtcaaag
     2221 a
//
LOCUS       DASRKK010000607         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_640693, whole genome shotgun sequence.
ACCESSION   DASRKK010000607 DASRKK010000000
VERSION     DASRKK010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640693"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFC15_14535"
     CDS             <1..711
                     /locus_tag="VFC15_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM11421.1"
                     /translation="VRHVVSAEAALAIVGECTPAVITLDIHLPGMDGWEFLGRLKKTA
                     AWEGVPIVVVSVLADRRAGFSLGAALVLQKPIGGDALAKGLKQLGLTSEGESETTVLV
                     IDDDVGAVELLAGQLHQRGCTVLRAPGGREGIELARRFRPDLIALDLEMPEVNGFEVV
                     EALKDDASTAQIPIVVVTAKDLTTQDRKRLNGHILEIVGKAEFDHGRFMGEVQRALSR
                     PGRGRTNGDVAGNERLHG"
     gene            1056..1433
                     /locus_tag="VFC15_14540"
     CDS             1056..1433
                     /locus_tag="VFC15_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11422.1"
                     /translation="MTSSRYSKELLTTLAVYALLLLGSVELLTHVPIGAPWRDAIALT
                     PMIGCSGAIWAILRELRRMDELQRRIQLEALGFAFAGTAFVTLSYGFLEGLGYARLTM
                     FLVWPLMASLWIVGLVLARRRYR"
     gene            1430..1624
                     /locus_tag="VFC15_14545"
     CDS             1430..1624
                     /locus_tag="VFC15_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM11423.1"
                     /translation="MRNRVRDLRSERGWSQTELGERLGVSRQTVNAIENERYDPSLPL
                     AFVIARVFALPIEKIFLEKK"
     gene            2238..4853
                     /gene="mgtA"
                     /locus_tag="VFC15_14550"
     CDS             2238..4853
                     /gene="mgtA"
                     /locus_tag="VFC15_14550"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZM11424.1"
                     /translation="MTHPENHSAARTGGSGSPVSDHLLRVSRADSKTALQELESRAEG
                     LSQAESGSRLKKYGPNEIAREKHQSSLMRLLSNVKNPLVLLLTALGVLSFLTGDLRAT
                     AVIFVMVVLGVVLRFIQEMRADNAAEMLKAMVSNTATLVRDGKDVDAPLKTLVPGDII
                     KLAAGDMVPGDVRVLSAKDLFLNQSALTGESLPVERNADASTADVQNPLALHNICFMG
                     SNVESGSATAVVIHTGDKTYFGSLAASIVGQRQLTSFDKGVNKFTWLMIGFIAVMVPA
                     VFLINGLVKHDWLEAFLFAMAVAVGLTPEMLPMIVTVNLSKGALAMAGKKVIVKRLNA
                     IQNFGAMDVLCTDKTGTLTQGKIVLEKHLDAHGDPSEKVLHYSYLNSFHQTGLKSLLD
                     EAILAHEELEERLKAKEEYRKIDEIPFDFVRRRMSVVVEDTTGLNTLICKGAVEEVLG
                     LCTKVEVHGEIIEVLPEHDAKRRQIADDLNGQGFRVIALAYKQMPGAADEPTYSVKDE
                     SDLILLGFLAFLDPPKETASNALKQLKGLSVDVKILTGDNEIITAYICKAVGMPVEHL
                     LLGTTIEAMSETELAEAASVTSVFARLAPTHKERVIQALQSKGHVVGFMGDGINDAPA
                     LKAADVGISVNSAVDIAKESSDIILLENSLLVLEQGVLEGRRVFGNIVKYIKMAASSN
                     FGNMFSVVGASAFLPFLPMLPIQVLTNNLLYDFSQTTIPTDAVDAEWLTKPRKWEIDR
                     ILRFILFIGPISSVFDYVTFFIMLYVFNAWQEPALFHTGWFVESLFTQTLIIHVIRTN
                     KIPFLQSRASLPLILTSILIVTVGAVLTVSPLAGVLGLVPLPSLYWLLLAVILVCYLI
                     LTQVVKTWFYRRFGE"
     gene            4963..5358
                     /locus_tag="VFC15_14555"
     CDS             4963..5358
                     /locus_tag="VFC15_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11425.1"
                     /translation="MRRVLTTLVEKVFVGSAQRPVGCLFRRNSVEVLVVGLRVCARMV
                     DDAVSMIRRRIERVELQRNAAGVGDVVIRPGRNDDRETRPNRRADAIENRLPGALLDP
                     EELVELVDFGPDLPLGLSAMMTSWQCFAV"
BASE COUNT         1042 a         1637 c         1654 g         1116 t
ORIGIN      
        1 gtgcgacacg tcgtgtcggc agaggctgcg ctggcgatcg tgggcgagtg cactccggca
       61 gtgatcacgc tcgacatcca tctgccgggc atggacggct gggagttcct cggtcgcctg
      121 aaaaaaacag cggcatggga gggcgtgccg atcgtcgtcg tgtccgtctt ggccgaccgc
      181 cgcgctggct tctcgttggg tgctgcgctg gtcctgcaaa agccgatcgg tggtgacgca
      241 ctcgcaaaag ggctgaaaca gctggggctg acttcggaag gcgagtccga gaccacggtg
      301 cttgtgatcg acgacgacgt gggtgcggtt gaactactcg ccggtcagtt gcatcaacgc
      361 ggctgcactg tgctgcgcgc gcctggcggg cgtgaaggca tagagctagc gcggcgcttt
      421 cggccggacc tgattgccct tgatctggag atgccggagg tcaatggctt cgaagtcgtt
      481 gaggcactca aggatgatgc ctcgaccgcg cagattccga tcgtcgtggt gacggcgaag
      541 gacctgacga cacaggatcg taagaggctc aacgggcaca tcctggagat cgtcggcaag
      601 gcggagttcg accacggaag attcatgggc gaagtgcagc gagcgctttc caggccgggt
      661 agaggtcgta ccaatggcga tgttgcgggc aacgaacgcc tccacggcta gtacctacct
      721 actcactgtc gagccgctgc agccggctgc tttcggattc gtcaagcgga gcatggagag
      781 ctcacagtcg acccgttgtt gacgatcggt tgcccaattt cgacggcgca aagcagtccc
      841 gtggagatga gcaacgtcgg cgtcggtctc ggccgaccat gtcatccgag cctgtgcgct
      901 cgcccactat cagcgcgagg atgcgccatc gatcgggagg agccacgaac ctcgctcgga
      961 agcgtggcgc cggaattgat gtgtaaagca tcctttacag gctatcggga tggcgtcaca
     1021 atggaaagca tcctttccac tcttgggatt ctgcgatgac ttcctcccgc tactccaagg
     1081 aacttctaac caccctggcc gtatacgccc tgttgctgct cggttctgtc gaacttctga
     1141 cgcatgtgcc aatcggcgcg ccttggcgcg atgcgattgc gctgaccccg atgatcggct
     1201 gcagcggcgc gatatgggca atcctccgcg agctgcgccg catggacgag ctgcagcgcc
     1261 gcatccagct cgaagcgctt ggcttcgcgt ttgcaggcac tgcattcgtg acgcttagct
     1321 acggctttct cgaaggcctg ggctatgccc gcctcacaat gttcttggta tggcctctga
     1381 tggcctcgct gtggatcgtc ggcctcgtgt tggcccgcag gcgctaccgg tgaggaatcg
     1441 cgtccgcgac cttcgcagcg agcgcggctg gtcgcagacc gagctcggcg agcggctcgg
     1501 cgtttcacgg cagacggtca atgcgatcga gaacgaacgc tacgacccga gcctaccgct
     1561 cgccttcgtg atcgcgcgag tcttcgcgct tccgatcgaa aagatcttcc tcgagaaaaa
     1621 gtgacttgcg ctgttcattg atcggtgccg tcgctgcggc ttgctacgct tccatcagat
     1681 agctgcgcat tccgaaagca gccagccacg aacgcccgca gctggccgag agcgtgagtc
     1741 caccgacgtc gccgagagca gcctttgacg ggtgaggtcc tgaattcgat ggtcaactct
     1801 ggggcgtgac tccgaactca cactcacggc cagaagcgag ccgccgtgac agaccgctgt
     1861 tcggcgggct cctgagggcg cccacgtgtc agtaaccggc tcgaagattg ccagcgctgt
     1921 tcccgcatag ccgaacgcgg cgagaatgaa ctctagttcc agggcactct atctgccgat
     1981 gggttgggtg gtggtggagg tggcgcccgc gcccctggtt gctcgcatgc cgagctcgtt
     2041 cctggtcttg ctcatctccg gcagcttgag gaattcgctc ggcgcgagcg tgttcctgtt
     2101 cgacaattgc gttcactgcg cgcatgtcgt ccggcacctg ttcgtgctgg gggcagtcgc
     2161 gccacttctt tgcggcgctg tggtacgcgt acgggagacg ccagtgcacc caccgagatg
     2221 cccatagggt ttcatccatg acgcatcccg agaaccacag tgccgctcgc accggcggaa
     2281 gcgggtcacc tgtatccgat cacttgctga gagtgtcgcg cgccgactcc aagactgcgt
     2341 tgcaggaact cgagtcgcga gcagaaggtt tgagccaagc ggaatccggt tcgcgattga
     2401 agaagtacgg gccgaatgag attgctcgcg agaagcatca gtcatccttg atgcgccttc
     2461 tgagcaatgt caagaatccg ttggtcctgc tgctcactgc gctcggcgtg ctgtcgtttc
     2521 tcacgggcga cctgcgcgct acagcagtca tcttcgtcat ggtcgtgctg ggtgtggtgt
     2581 tgcgcttcat acaggagatg cgggctgaca acgccgccga aatgctgaaa gctatggtca
     2641 gcaacaccgc gaccttggtg cgcgatggca aggacgtcga cgcgcccctc aagacgctgg
     2701 tgccgggcga catcatcaag ctcgcggcag gcgacatggt acccggcgat gttcgggtgc
     2761 tttccgccaa agacttgttc ctgaatcagt cggcactcac cggtgagtcg cttcccgtgg
     2821 agagaaatgc cgacgcctcg accgccgacg ttcagaaccc actggcgctg cacaacatct
     2881 gtttcatggg ttccaacgtg gagagcggca gtgccacagc cgtcgtgatc cacaccggcg
     2941 acaagaccta tttcggctcg ctggccgcaa gcatcgttgg acagcgccaa ctgaccagct
     3001 tcgacaaggg cgtgaacaag ttcacttggc tgatgattgg attcatcgcg gtgatggttc
     3061 cagcagtctt cctgatcaac ggactggtca agcacgactg gctagaggct ttcctgtttg
     3121 ccatggcagt ggctgtcgga ctgacgcccg agatgctgcc catgatcgtg acggtcaacc
     3181 tttccaaagg tgccctggcg atggcaggca agaaggtgat cgtcaaacgc ctgaacgcca
     3241 tccagaattt cggggcgatg gacgtactgt gcaccgacaa gaccggtacg ctgacccagg
     3301 gcaagatcgt gttggaaaag catcttgacg cgcacggcga ccccagcgag aaggtcctcc
     3361 actacagcta cctgaacagc ttccaccaga ccgggctgaa gagtctgctc gacgaggcga
     3421 tcctcgccca cgaagagctg gaggaacgcc tgaaggccaa ggaggaatac cgcaagatcg
     3481 acgagatccc cttcgatttc gtgcggcgcc gcatgtcggt ggtcgtggaa gacacgaccg
     3541 gattgaacac gctgatctgc aagggtgccg tcgaagaggt gttgggcctg tgcacgaagg
     3601 tggaagtcca cggcgagata atcgaagtcc tgcccgagca cgacgccaag cgtcgccaga
     3661 tagccgacga cctgaacggt cagggcttcc gcgtgatcgc cttggcctac aagcaaatgc
     3721 ccggagcggc ggacgagccc acctactccg tcaaggatga gtccgacctc atcctgctcg
     3781 gcttcctggc ctttctcgac ccacccaagg agaccgcctc caacgccctg aagcagctca
     3841 agggcctgag cgtggacgtc aaaatcctga ctggcgacaa cgaaatcatc accgcctaca
     3901 tctgtaaggc agtgggcatg ccggtcgagc acctcttgct cggcaccacg attgaggcga
     3961 tgagtgaaac cgaactagcc gaggctgcca gcgtgaccag cgtcttcgcc cggctggccc
     4021 cgacgcacaa agaacgtgtc atccaggcgc tccagagcaa gggccatgtc gtggggttca
     4081 tgggtgatgg tatcaacgac gccccggcgc tgaaggccgc agatgtcggc atctcggtga
     4141 acagtgccgt ggacatcgcc aaggaatcat ccgacatcat cctgctcgaa aacagcctgc
     4201 tcgtcttgga gcagggggtg ctggaaggcc gacgggtatt cggcaatatc gtcaagtaca
     4261 tcaagatggc agccagctcc aacttcggca acatgttcag cgtcgttgga gctagcgctt
     4321 tcctgccgtt cctccccatg ttgccgatcc aggtgctgac caacaacctg ctttacgact
     4381 tctcgcagac caccattccc accgacgcgg tagatgccga atggctgacc aagccgcgca
     4441 agtgggagat cgacagaatt ctgcgcttca tcttgttcat tggacccatc agctcggttt
     4501 tcgactacgt gacatttttc atcatgctgt acgtgttcaa tgcttggcaa gagccagcgt
     4561 tgttccacac gggctggttc gtcgagtcgc tctttaccca gacgttgatc attcacgtca
     4621 tccgcaccaa caagattccg ttcctccaga gccgcgccag cttgccactc atcctgactt
     4681 ccatactcat agtcacggtg ggcgctgtgc tcacggtttc tccactggcc ggcgtgctcg
     4741 gtctggtgcc gctgccttca ctgtactggt tgttgctggc tgtcatactg gtttgctacc
     4801 tgatcctgac tcaagtggtc aagacatggt tctatcgccg gttcggcgaa tgatggtgat
     4861 gcgataggtt gcggtcactc accacatcgc gccattgagt cactgcattt ccatcgctgg
     4921 ccagggtcac cttgaattga tcctcgttca tagcgagacc tcgtgcgtag agtgctgacg
     4981 acgctggtcg agaaagtctt tgtgggatcg gcccagcgcc cagtcgggtg tctattccgt
     5041 cgtaattccg tcgaagtgct cgttgttgga ctccgcgtct gcgcgcgtat ggtggacgac
     5101 gccgtctcga tgattcggcg gcgcatagag cgtgtagagc ttcagcggaa cgctgccggt
     5161 gttggtgatg ttgtgattcg ccccggccgg aacgacgacc gcgaaacccg cccgaatcgg
     5221 cgtgcggacg ccatcgagaa ccgcctcccc ggtgccctcc tcgacccgga agaactggtc
     5281 gagcttgtgg acttcggccc cgatctcccc ttgggcttga gcgccatgat gacgagttgg
     5341 cagtgcttcg ccgtgtagag cacccgacga aactcctcgt tgctgacggc gagaccctcg
     5401 atgtcctgca caaagccttt catagcgact cctctttcgt cacctcaat
//
LOCUS       DASRKK010000608         8132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1032263, whole genome shotgun sequence.
ACCESSION   DASRKK010000608 DASRKK010000000
VERSION     DASRKK010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032263"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1817..4537
                     /locus_tag="VFC15_14560"
     CDS             1817..4537
                     /locus_tag="VFC15_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZM11426.1"
                     /translation="MLDATACRLAPEVATVLGQDDLLAWFQRLSIPEPTCSMIDHIRS
                     SNPSRRVGGGRSNVSGRYPSRKMGVTIQFESHRVELAGIYEMEHDATVLEYFDQPPAI
                     KLDYESVAGKRMGVLHTPDFFVIRRGEAGWEEWKTEEELRRLNMRNPNRYAAGSDEPW
                     RCPPGQAYAERLGLYYRVRSSAEINWVFQRNIQFMEDYLRADLTAIAAGSRDIVIACV
                     SANPGLALEDLLRSTAGRVPPDDIFSMIAANVVYVDLHAAPLAEPSRVAVLVAPGATP
                     KARDNGTRKLSSCSFFDLHCGSTVTWDDRFWNVVNIGETSVGLLSDDQRLTELPKTAV
                     EALICQNRMQLISPGSDHESDSAICKLLSGAREHDLYVANQRARLIRDYLANGRLPAE
                     LKVTGRTFLRWLARYRAAEGNLGVGYLGLLPKSGDRGNCTPRLSEASLRLMKEFLEQD
                     YETLKQKTRYASWVGLKLACEHQGVAAPSYKTFCVAVKNRPALNQTLKRQGRRASYKL
                     ETFYWELDQKTPRHGDRPFEIVHVDHTELDVESVASTGQVLGRPWMTIATDAFSRRIL
                     AFYLTFDAPSYRSCMMALRECVRRLSRLPQILVVDGGREFESTYFETLLARYECTKKT
                     RPPAQARFGAVCERLFGTTNTQFIHNLRGNTQITRNVRQVTKSVDPKSLATWPLAELH
                     QRLSQYLYEVCDTFTHPALGQSPREAFETGLALTGSRLHRLVPYNEEFLMLTLPTTAK
                     GTAKVMPSRGVKINHICYWCEAFRDPEVQCASVPVRYDPFDAGMAYAFVRKQWLQCHS
                     EYYAVLKGRSEREIMLATNELHQRRRNHSAAFTVTARQLAEFLQSVEAEEKLLTQRLS
                     DLESRDIRLTLAKRPFNEGCAPCREADVPAAQSSGQPVDETTGSEVYGAF"
     gene            4678..5742
                     /locus_tag="VFC15_14565"
     CDS             4678..5742
                     /locus_tag="VFC15_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM11427.1"
                     /translation="MAPNSLILVMGPTGVGKTTLRAKVEQMLTTEMLPELGADPVRLP
                     VVSVECIAPESGTFSWRDHFRRLLLQMDEPLVDYKLNPEAPVRMGDRATRFLPGVRAA
                     GSQFHHAAERALRFRRPAAVLLDEAQHLARMSSGRRLSDQLDVIKSLANCTGTVHVLL
                     GTYELLAFRNLSAQLSRRSVDIHFPRYRVDDPNDQKIFLTVLRSFEQQLPLSEPPKLV
                     EEWEYLYERSIGCVGVLKDWLVRALTGVFRRNASVLTLRDLQTHALSVPQCDKMLSEA
                     LEGEVRLLESTEQRNRLRTRLGLGPQETYREKSSSGVTTQVAVEITSPRKRRRKPGQR
                     QPRRDPIGLPTTSYATAINV"
     gene            6485..7654
                     /locus_tag="VFC15_14570"
     CDS             6485..7654
                     /locus_tag="VFC15_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11428.1"
                     /translation="MANFRACVDAVAEGNRLAFARASCISPCAVHYQMVGKSLPQIGT
                     LLRICYHLDVPLTALLESDSIHLAAGLAQAKDAIQRNRKRPLSRTPEQVRFVLEKALR
                     EQPAPSLSEIVRRLGYKGAERLYQVDQNLAKQIVANYRKSGRSHWWRKPGAVRICALS
                     DIQKLLEQSLAQEMPVSAHHIAASLGYANDGYLQQKFPDLCRAIRRKIAAHDEPRATI
                     MENALEDALREDQVPTLSDLCRRLGYSNSLVLRYHFPDLCDEILARRRDLRDQQIAGL
                     RKTLQAVLLEEPAPSFRSVCKRIGRSNAALTETCPEEGAAIRSRYLRSRSEASRRRKE
                     QLGKEVRHIVQRLQAEGRCPSVDRVTALLPNTALKDWRTISAWVKAARQELGPAQ"
     gene            7936..>8132
                     /locus_tag="VFC15_14575"
     CDS             7936..>8132
                     /locus_tag="VFC15_14575"
                     /inference="COORDINATES: protein motif:HMM:NF039653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11429.1"
                     /translation="MVEITKDTGGHLTGIELDADRATAAAGKGIATVHGSAFECRVLA
                     ETCSLLYLNPPYDSEFGPHSN"
BASE COUNT         1800 a         2291 c         2347 g         1694 t
ORIGIN      
        1 tcgtgggccg gttcgcagtc atgagagtgc tgcggagcgg ctggtcagaa tcgtaaacag
       61 tgagcgtgac ggttgcactt tgttgcagcg caacccacac cgaaacagca tctttcgtga
      121 cctggcgtaa gattgggcca gccaagacga ggggcagttt cggcaatcga gtgagtagac
      181 catccatcac taaagccaac cttcaacttg ctgttagccg gagtctatcg agtgaatgcc
      241 tcgcggacag acgatagcag ggcaaacaga ggccaccctc caacaattcc atcgacagca
      301 aattacaatg attgcgaggc acttccagag agtgccgaca agagcggaag tttcagcagc
      361 tgcgttcagc ttcgccttga gtatcgggag tataccttga aagtgcagca gtcgttaggg
      421 aaatctttcg acctattctt gaactcgtgt ttctggcatt ttgcgtgacc gtttgtttct
      481 ccaaccccgt caaagcagac gccctgttta tgcgcggacc tatcttcgtt gaatgcatgg
      541 acgaagacat gccaagcggc gcgcttttta ccagaacgac agaatcggtg actcgtccgc
      601 catcaagccc tctccgaata acgtgtgtcg cgtgcgcacg aatctaagtt cgttcggcct
      661 cgattggtcg tgccggcaaa tgagccagag tcgtttaaca cagggcagtc tccgtccgaa
      721 tttcaacccc agataatgga tccacgtttt cgccggcgat cacattttcc aacctcgtac
      781 gttcggctga tatccagaaa tccaagtgtt ccactcctca actggcggta acaggccaag
      841 tcactgattg agcctttcgg atcgccttcg cctctggcaa gttgacgtat aacgcccaaa
      901 ccgcggatct gccccgggga gtgcgggcct gaagtcccca tcgaggaagt tgaccagccc
      961 gattccagcc agacgacggc gtgcccggaa gttctctcgc cggttctgta ggcgctagac
     1021 gcaactgggc aaggtaacgc aatttgcgga aatttagcgc atcgtgcccg gcatccccgc
     1081 cgccaccgtt aattgagcac cgaagtaaac cacaccgttg ccattcgcca agactctgct
     1141 tcaagtcaca ccatctgtgc cttgaaacat aaaatgtcac gggattgaag cataaaatgt
     1201 cactgccgca ccagaaaccc cttttgaatc agtgcggttt ctttcggtcg gcaacccccg
     1261 tttgaagcat aaattggcac taagtacaga acggaaggaa aaagtgtcag taagtgcaat
     1321 tgggccgcga cgcacacttg ggcgcgtcct ccggaactcg gcgcaccgaa cggcgccctg
     1381 ccgggtgcac ctgcccaacg gtgatggtct cggcgaaggt cggaaccagg gcagtaattg
     1441 acaacgctta cgtacaggtg ctatattaca cctatacatg aacaagaacg aaacatcgga
     1501 agatgtacgg cgttggttct tgcgcgctat caaggatttg tggccggttg ctctgggttc
     1561 tgtttccctg cgaaagagtc cctgcattcg ggagaactgt tctgcttgcg catcgggtca
     1621 aggccatagc agctacgcgc tgtcgggcta ccggggaaat cgccggttct ctgtctacgt
     1681 gccggacgaa ttagctccag acattcagcg ggccctcgaa aagggccggc gggtacagga
     1741 cttgctcaag gaggccgggg tgcgttactt gcgtgccgag aagcgggaga ggcgctccaa
     1801 accgcagaag tgagagatgc tggacgcaac tgcttgccgt ctagctccgg aggtggccac
     1861 tgtgctgggg caagatgact tgttggcatg gttccaaagg ctgagcattc cggaaccaac
     1921 gtgctcgatg atcgatcaca ttcggtcgtc gaatccgtcg cggcgcgtgg gcggtgggcg
     1981 ctcgaatgtg agcggccgct atccgagccg gaagatgggc gtcaccatcc agttcgaaag
     2041 ccaccgcgtc gagttggctg ggatctacga gatggaacat gacgctaccg tcctcgagta
     2101 tttcgatcag ccgccggcaa tcaagttgga ttatgagagc gtcgcgggga agcggatggg
     2161 ggtgctgcac acgcctgact tcttcgtcat ccgccgcgga gaagcgggtt gggaagaatg
     2221 gaagaccgag gaagaactgc gtcgactcaa catgcgcaac cccaatcgtt atgctgctgg
     2281 aagcgatgag ccgtggcgtt gcccgcctgg ccaagcctac gcagaacggt tggggctcta
     2341 ttatcgtgtc cgctcctcgg cagagattaa ctgggtgttt cagcggaaca ttcagtttat
     2401 ggaagattac ctgcgggccg atttaaccgc gatcgcagcc ggcagccggg acattgtcat
     2461 tgcatgtgtg tcggcaaacc ccggcttggc gctggaggat ttgttgcgat caaccgccgg
     2521 cagggttccg ccggacgata tctttagcat gatcgcggcg aatgttgttt acgtagatct
     2581 ccatgccgca ccgctagccg agccctcgcg ggtcgcggtc ttggtagcgc caggagcaac
     2641 tcccaaagca agggacaacg gaacccgaaa actcagttcc tgctcctttt ttgatcttca
     2701 ctgcgggagc acagtgacct gggacgaccg cttctggaac gtggtaaaca tcggcgaaac
     2761 gtccgtaggt ctactctctg acgatcagcg actaaccgag ttgccgaaaa ccgcggtcga
     2821 ggctttgatc tgtcagaatc ggatgcaact aatttctccc ggctccgacc acgaatccga
     2881 ttccgccatc tgcaagctcc tctctggggc acgcgagcat gatctgtacg ttgctaatca
     2941 gcgagcccgg ctaattaggg actatctggc gaatggaagg ctgccagcgg agctcaaagt
     3001 gaccgggcgc actttcttgc gctggcttgc tcgataccgc gcagcagagg gcaacttggg
     3061 tgtcgggtat ctcggtctgt tgccaaagtc cggcgacaga gggaactgta cgccgagatt
     3121 atccgaagcg tcgctgcggc tgatgaaaga gtttctggag caggattacg agacgttaaa
     3181 acagaaaacc cggtacgcat cgtgggtagg gctgaagctc gcctgtgagc accagggagt
     3241 cgccgctccc agctacaaaa ccttctgcgt cgcagtgaaa aaccgaccag ctttgaatca
     3301 gacactaaag cgacaaggtc ggcgggccag ctacaagttg gagacgtttt attgggagct
     3361 ggaccagaag acaccgcgac acggtgatcg cccctttgag attgtccatg ttgaccacac
     3421 ggaactggac gtcgagtcgg tcgccagtac cggtcaggtt ctcggtcggc catggatgac
     3481 gattgccaca gacgcattct cgcggcggat cctggctttt tacctgacat tcgatgcacc
     3541 gagttaccga tcctgcatga tggcgctgcg ggagtgcgtg cgacgactta gtcggcttcc
     3601 gcagatcttg gtggtcgacg gaggacggga gtttgagagc acgtacttcg aaactttgtt
     3661 ggcgcggtat gaatgcacga aaaaaaccag gccgcctgct caggcacgat ttggggcggt
     3721 ttgcgagagg ctgttcggaa ccaccaacac ccagttcatc cacaatctgc gggggaacac
     3781 ccagatcacc cgcaatgttc ggcaggtcac caagtccgtg gacccgaaaa gcctggcaac
     3841 atggccgctg gcagaactgc atcaacgatt atcgcaatac ctctacgaag tctgcgacac
     3901 cttcacccat cctgcgctcg gccagagtcc acgggaggcg tttgagactg gcttggcctt
     3961 aactggcagt cggcttcatc gactggtccc ctacaacgaa gagttcctga tgctgacgct
     4021 gcccacgact gccaaaggaa cagcgaaagt catgccctcg agaggagtga agatcaatca
     4081 catctgctac tggtgcgagg cttttcgcga cccagaagtc cagtgtgcat cagtgccggt
     4141 aagatacgac ccttttgatg ctggcatggc ttacgccttt gtgcgtaaac aatggctgca
     4201 gtgtcactct gagtactacg ccgtattgaa gggccgctcg gagcgggaga tcatgctggc
     4261 caccaacgaa ctgcaccagc gtcgccgcaa ccattccgcc gccttcaccg tcaccgcccg
     4321 acagcttgct gagtttttgc agtctgttga ggccgaagag aagctgctga ctcagcggct
     4381 aagcgatttg gagagcaggg atattcggtt gacgctcgcg aaacgacctt tcaacgaggg
     4441 ctgtgcccct tgccgagagg ccgatgtgcc ggctgcgcaa agttctgggc agccagtgga
     4501 cgagacgact ggcagtgaag tatacggagc attctgatgg atacggggtc ggcaggattc
     4561 cctcgtgaac tactttccca gccttggcct gcgaggctcg gatattttcg ggcatacacg
     4621 atggctcatc cacgactgct tgcggctcga gaaaccctcc tgagtgcgat tcacgaaatg
     4681 gcgccgaact ccctgattct ggtaatgggg ccaaccggcg taggaaagac aaccctgcgg
     4741 gcaaaggtcg agcagatgct gaccaccgag atgctgccag aactcggagc tgatcccgtg
     4801 cggctgccag ttgtgagtgt ggaatgcatc gccccggaat cgggtacttt cagttggcga
     4861 gaccactttc gaagactact gcttcagatg gacgagccct tggtcgatta caagctgaat
     4921 ccggaggcgc cggtccggat gggagaccga gctacaaggt tcctgcccgg cgtgagagca
     4981 gcagggtcac agttccatca cgccgcggag cgggctcttc gctttcggcg gcctgcagct
     5041 gtactccttg acgaggcaca gcacctggca agaatgagtt ctgggcgaag gctttcggac
     5101 cagttggacg ttattaagtc ccttgctaat tgcaccggga cagtccacgt gcttttgggc
     5161 acctatgaac tccttgcctt tcgcaacctg agtgcgcaac tgagccgccg gagtgtcgat
     5221 atccactttc cgcgctatcg agtcgatgat cccaatgacc aaaagatatt cctaaccgtc
     5281 ttgcgttcgt tcgaacaaca gcttcccctc tcggaaccgc cgaaactggt agaggaatgg
     5341 gagtatctct acgagcgcag catcggttgc gtgggagtgc tgaaagactg gctcgtcagg
     5401 gcgctcactg gagtcttccg gcggaacgct agcgttttga ccctcagaga tttgcagacg
     5461 catgcgctga gcgtgcccca atgcgacaag atgctgtcgg aagccttgga aggagaggtg
     5521 agattgttgg agagcacgga gcagcgcaac cgcctgcgaa cacgactcgg cctgggtcct
     5581 caagaaactt atcgtgaaaa gtcgtcaagc ggcgtgacga cccaagttgc tgtcgagatt
     5641 acgtcgccac ggaagcgacg taggaagccg ggacagcgac agcctcggcg tgatcctatc
     5701 ggtctcccaa caacgagcta tgcgactgcc attaacgttt gactcctggg actgcaagct
     5761 accgccctta ccgatccgaa gccggctata tgcgctggag cccatcggga ttggtactcc
     5821 attcgtcgag agtctttctg gctacgtggc ccggctcgcc gatgcccacg cggtctcagt
     5881 gggtgacttg gcgggtcgag aactatccgc cctcacatcg agaccactgc tctccttcgg
     5941 accgtttatg cgacgaaacc gggctgattc ccacggcttt cacgcgcagc agtatacgat
     6001 gaatggcctt ggaatcacgt cgaagaagtg ggttgaagcc ctggaacggg ccactctgcg
     6061 cacagatctt cggttcctca cactgctgcc atttgagggc gtgcttagtt gggtggctgc
     6121 gtttcgctgc aaccgtgctt ggtgtcctgc ctgctatgag gattggcgaa gtgctggaga
     6181 tgtcgtatac gagccgctcc tgtgggctat cgatccggtc acggtttgct cacggcatcg
     6241 tcgaccttta gaggaaactt gcggatattg ccatgggcgg ctgatgccgc ttgccgtttt
     6301 ctcgcgtcca ggctactgct cgcggtgcca ggcatggctc ggggacaaca aagctggcag
     6361 cgcacctggg caaccaactt gggtcccaga ggaaaatgat gtagccctct ggcgtgctaa
     6421 ggcgatcggc ggactgctcg ccaccgctcc gcgcctacat ggctcctctt taaggactgt
     6481 attcatggcg aacttccggg cctgcgtgga cgcggttgcc gaaggcaacc gacttgcctt
     6541 tgcccgggcc tcttgcatat cgccgtgcgc cgttcactat cagatggtcg ggaagagcct
     6601 gccacagatt ggtacattgt tgcggatctg ctaccacctc gacgtaccgc tgacggctct
     6661 tctggaaagt gattccatcc acctcgctgc gggtttggcg caagcgaagg atgccatcca
     6721 aagaaatcga aaacgtcctc tatcccgcac ccccgaacag gtgcgcttcg tactcgaaaa
     6781 ggcgctgcga gagcaaccag cccccagtct ttctgagatt gtccgacggc tgggctacaa
     6841 aggagcagag cgcctgtacc aagtggacca aaatctcgca aaacagatcg tcgccaacta
     6901 ccgaaaatca ggacggagcc attggtggcg gaaacccggg gcagtgcgaa tctgcgcgct
     6961 ctccgacatc caaaagctct tggaacaatc gcttgctcaa gaaatgccgg tctccgccca
     7021 tcacattgca gccagtctgg ggtacgcaaa cgatggctat cttcaacaga agttccctga
     7081 tctctgtcgc gcaattcggc gaaagatcgc ggctcacgac gagccacgcg caaccattat
     7141 ggaaaatgct ctcgaggatg cgcttcggga ggatcaggtt ccgaccctaa gcgacctgtg
     7201 cagacggctc ggctactcta actcacttgt tttgcgatac cactttcccg acctttgcga
     7261 cgaaatactg gcacgccgac gcgatcttcg tgatcaacag atcgccgggc tgcgaaaaac
     7321 gctgcaggcc gtgcttctgg aagagcctgc tccatcgttc cgcagcgtgt gcaagcggat
     7381 cggccgttcg aatgcggctc ttacggaaac gtgtcccgaa gagggtgccg cgatccgctc
     7441 aagatatctg cggagccgaa gcgaagcctc gcgacgcagg aaagagcaac tgggcaaaga
     7501 agttcgtcat attgtgcaga gacttcaagc cgagggcagg tgtccttccg ttgatcgggt
     7561 gacagccctc cttccgaaca cagcgctcaa agactggcga actatcagcg cgtgggtaaa
     7621 agccgcgcga caagaacttg gtccggccca gtagaagtgc agacccctcc gggccgtcgc
     7681 acctcctgca atagacatcg gacaactgca ccgtcacgtt ctttttgaag cataaagtgt
     7741 cacgcggtga cattttatgc ttcaagtcac acgctagaaa cacggcaccg ttctggagga
     7801 aaaatgagaa atgcgggccg cctcaaactc ggatattacc ctttgcctgt ctaagaagcc
     7861 cggaacattc gggaactgtt gttaccgaca gcaccatatg cggccatcga tccttgtgtc
     7921 ggcgacggta cggcattggt cgaaatcacc aaagacaccg gcgggcacct gaccggcatc
     7981 gaactcgatg cagacagggc cacggcagcc gccggaaaag gaatcgccac cgtccacggc
     8041 agcgcttttg agtgccgggt gttagcggaa acttgttcgc tcctgtatct gaatccgccc
     8101 tatgacagcg agtttgggcc acacagtaac aa
//
LOCUS       DASRKK010000609         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1325123, whole genome shotgun sequence.
ACCESSION   DASRKK010000609 DASRKK010000000
VERSION     DASRKK010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325123"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFC15_14580"
     CDS             complement(<1..1037)
                     /locus_tag="VFC15_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11430.1"
                     /translation="MQPHIERIDVSTEELEGLLDQARPALSEDGYQKLRAAIRTLGYV
                     TGLLENQETTLASLRELLCHASTEKTEAVLKQAGIETGDKKRKAPKEHAGKSAPSSGH
                     GRNGAAAYRGAQKVQVSHESLKAGDQCPDAECDGKVYAQRDPGVLVRVKGQAPIAATV
                     YELEKLRCHLCGMVFTAAAPAGAGEKKYDESAAAMMALLRYGSGFPWNRLEGLEENLG
                     IPLPAATQCQIMTETAVPLQPALDELIRQAARGEVAHNDDTSMRVLSLDRDADLSPER
                     TGVFTSGIVWVAGKHRIALYFTGCKHAGENLAEVLKQRPPGLSPPIQMCDALSRNVPK
                     LNPTLVANCNA"
     gene            complement(1022..1918)
                     /locus_tag="VFC15_14585"
     CDS             complement(1022..1918)
                     /locus_tag="VFC15_14585"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HZM11431.1"
                     /translation="MLETWRYRGQEISGEQIAFLREFIRAHPTSSRWKLSRQLCEALD
                     WKQANGALRDVVCRGLLLMLERAGEIELPPVRRLIRGQRRTGRPRPEAVLMDTTPLTT
                     TLKALGPIDTLPVRRTADEPLFNSLMEHYHYLGYEQPVGEHLKYLIWAGGRPIACLAW
                     SSAPRHLASRDHYLGWSAEARRRNIRFIAYNTRFLILPWVEVPHLASHILGRMAARIS
                     KDWQALYGHPIYFLETFVDPERFRGTCYRAANWVLLGKTTGRGKQSNSYVPNRSIKEV
                     LGYPLTRRFRQLLEETEGCSHI"
     gene            complement(1964..2326)
                     /gene="tnpB"
                     /locus_tag="VFC15_14590"
     CDS             complement(1964..2326)
                     /gene="tnpB"
                     /locus_tag="VFC15_14590"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZM11432.1"
                     /translation="MGRGFVIQIAPQVRILVAIEAIDCRKGIDRIAQLCREKLNADPF
                     SGHLFIFRSRRGTSIRILQFDGQGFWLATKRLSKGRFKWWPTGNEAARVLQAHQAQLL
                     LAAGNPETEAAPVWRKVS"
     gene            complement(2308..2703)
                     /locus_tag="VFC15_14595"
     CDS             complement(2308..2703)
                     /locus_tag="VFC15_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11433.1"
                     /translation="MRGRKPADILDIDEVRTHFENWRQTRKGKARIPDELWSAAVEVA
                     RRDGVNQTAAALHLDGGKLKRRMMATGPVPDKTMPPAFVEMMVPQAVGASECTIELEG
                     RNGKLRIHWKGATAADLAGLSRALWDAAS"
BASE COUNT          510 a          987 c          820 g          572 t
ORIGIN      
        1 tgagcgttgc agttcgccac cagtgtgggg ttgagtttcg gcacattcct cgatagcgca
       61 tcgcacattt gaatgggggg cgaaaggccc ggaggccgct gtttcagcac ctccgccaga
      121 ttctcgccgg cgtgtttgca gccggtaaaa tacagcgcaa tgcgatgctt ccccgccacc
      181 catacgatcc cgctggtgaa caccccggtc cgctccgggg acaggtccgc gtcccggtcc
      241 agcgacaaca cgcgcatcga agtatcgtcg ttatgcgcca cttctccgcg cgcagcctgg
      301 cgtatcagtt cgtcgagcgc gggctgaagc gggaccgccg tttccgtcat gatctggcac
      361 tgcgtggccg ccggcagcgg aatgcccaga ttctcttcga ggccttccaa ccgattccag
      421 ggaaacccgc tcccgtatcg cagcagtgcc atcatggccg cagccgactc atcgtatttc
      481 ttctcacccg cgcctgccgg agcggcggcg gtgaagacca tgccgcagag atggcagcgc
      541 agcttttcca actcatacac cgtcgccgcg atgggtgcct gccctttgac ccgcaccagc
      601 acgcccggat cgcgctgcgc ataaaccttg ccgtcacact ccgcgtccgg acactggtcg
      661 cccgccttca aggactcgtg cgaaacctgg accttctgcg ccccgcgata cgcggcggca
      721 ccgttgcgcc cgtgccccga ggatggcgca ctctttccag catgttcctt tggcgctttg
      781 cgtttcttat cgccggtctc aatgccagcc tgcttcagca ccgcctcggt cttctccgtg
      841 ctggcatggc acagcaactc ccgcagcgac gccagagtcg tttcctggtt ctccagcagc
      901 ccggtcacgt agcccagtgt gcgaatcgcg gctctcagct tttgataacc gtcttctgag
      961 agtgccggtc gggcctggtc caacaatccc tccagttcct cggtggaaac gtcgattcgc
     1021 tctatatgtg gctgcatcct tctgtctcct ccagtaactg ccggaagcgc cgcgtcaggg
     1081 gataccccag cacctcttta atcgaacgat tcggcacgta gctgttggat tgtttacccc
     1141 gtcccgtggt cttgccgagc aacacccagt tggccgcgcg ataacaagtg ccgcggaatc
     1201 gctcgggatc gacgaaggtt tccagaaagt agatcggatg cccgtacagg gcctgccagt
     1261 ctttcgagat acgcgccgcc atccgcccca gtatgtgcga cgccaggtgc ggcacttcca
     1321 cccacggcaa aatcagaaaa cgcgtgttat aggcaatgaa gcggatgttg cgccgacgcg
     1381 cttcggcgct ccagccgagg tagtggtcgc ggcttgccag atgccgcggc gccgaactcc
     1441 acgccagaca ggcgatgggg cggccaccgg cccatatcag atacttcagg tgttcgccca
     1501 ccggctgctc atagccgagg tagtggtagt gctccattag gctgttgaac agcggctcgt
     1561 ccgccgtgcg ccgcaccggc agggtgtcaa tcggtcccaa cgccttcagc gtcgtcgtca
     1621 gcggcgtggt gtccatcagg actgcctccg gccgcgggcg ccccgtccgg cgctggcccc
     1681 ggatgagccg acgcacgggc ggcaactcga tctctccggc ccgctccagc atcagcagca
     1741 ggccgcggca aaccacgtcc cgcaacgcgc cgttggcctg cttccagtcc agggcttcac
     1801 acaactgccg cgacagcttc caacggctgc tggtgggatg cgctcgaatg aactctctca
     1861 gaaaggcgat ttgctcgccg gagatctcct gcccacggta gcgccacgtt tccagcataa
     1921 ccgcagcatg ccagaagaat aatcagaacg caagggtttt gtttcagctc accttccgcc
     1981 acacgggtgc cgcttcggtc tccgggttgc ccgccgccag cagcaactgc gcctggtgcg
     2041 cctgcaacac ccgcgcggct tcgttgccgg tcggccacca cttgaaacgc cctttggaca
     2101 atcgcttggt cgccagccaa aacccctgac cgtcgaactg gaggattcgg atggaggttc
     2161 cgcgccggct gcggaagata aacaggtggc cggaaaatgg atcggcgttc agtttctccc
     2221 ggcacaactg cgcgattcga tcgataccct tgcggcagtc gatggcttca atcgcaacca
     2281 gaatgcggac ctgtggtgcg atctggatca cgaagccgcg tcccacaaag cccggctcaa
     2341 cccggccagg tccgccgccg tggcgccctt ccagtggatt cgcaacttgc cgttccggcc
     2401 ttccagttcg atcgtgcact ccgacgcgcc caccgcctgc gggaccatca tttccacaaa
     2461 cgccggcggc atcgttttgt cgggcactgg gccggtcgcc atcatgcgcc gcttcagctt
     2521 tccgccatcc aggtgcagcg cggcggccgt ctggttgacg ccatcccgcc gggctacctc
     2581 gacggccgct gaccacaact catcagggat acgggctttc cccttccggg tctgcctcca
     2641 gttctcgaag tgggttcgta cctcatcgat gtcgagaata tctgctggct tacgacctct
     2701 catcgtcagt cctcctcgcc gatccgaccg cggaaagaat gacattagca gttatgcccg
     2761 gccgacttca tgcaggcttg ctgaaaactc acgtagttcc gtagctcctg cttacccaag
     2821 gcattgacac ccagggagag gcttcgaggc tggatcctat ttggaaccct gtgacgcata
     2881 acggaagac
//
LOCUS       DASRKK010000610         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_152321, whole genome shotgun sequence.
ACCESSION   DASRKK010000610 DASRKK010000000
VERSION     DASRKK010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152321"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFC15_14600"
     CDS             <1..558
                     /locus_tag="VFC15_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11434.1"
                     /translation="IFAHSPVSLEHQHLAKRYVVRGLESGGAAGELGRYVTFTQEDGQ
                     PIEWLHPVEAIGVNGLHAVVVAPVLVQVEMLRKGRTYELLITQHRPGATNDGKRPPMG
                     TRLLFRGIHGRLDLDLSGKDKAGTVLPTFYSRAGEEVMIPKRFRSVVRAIVGAVHCDG
                     CFHCHYVRPPQNKEDQSCAAERVTA"
     gene            587..715
                     /locus_tag="VFC15_14605"
     CDS             587..715
                     /locus_tag="VFC15_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11435.1"
                     /translation="MHRKVKNYSLVFCLFVFLFVIIGGGLHLIQAVHSLLQLPELH"
     gene            800..1435
                     /locus_tag="VFC15_14610"
     CDS             800..1435
                     /locus_tag="VFC15_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11436.1"
                     /translation="MAATDRITPIDESEVAVNESAGSECSEPTRAVRRHLPDERRALT
                     HHFSIAGQEGYLTVGVYEDGVPGEIFITMAKQGSTISELMDAFATVVSLALQHGVPLR
                     VLCDKLCHMRFEPSGWSGNPKIGYAKSLMDYIARWLELRFLNSDQGELFKALPMPVQP
                     AIDANGTDPVGALGEFIQMGDAPACNICGSLMVRSGTCYRCMTCGGASGCS"
     gene            1612..1818
                     /locus_tag="VFC15_14615"
     CDS             1612..1818
                     /locus_tag="VFC15_14615"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZM11437.1"
                     /translation="MVHSDPEIMAGTPVFRGTRIPVYLIADMMEQGTPINEILEGYPS
                     LTRDMVEYASVFATLHPRPDRQTR"
     gene            1995..2273
                     /locus_tag="VFC15_14620"
     CDS             1995..2273
                     /locus_tag="VFC15_14620"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HZM11438.1"
                     /translation="MPRVAVEDNSRMSLRIRPEEKALLLRAVALNHTDLTDFVIRHAL
                     RAAKAVIEEADHVQLSARDSVRVLELLENPPAPSRKLLAAARALPKRS"
     gene            2270..2797
                     /locus_tag="VFC15_14625"
     CDS             2270..2797
                     /locus_tag="VFC15_14625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM11439.1"
                     /translation="MIVTAWHEESINKTHDREAFDCGEEALNEFLRRYARKSHDLGGA
                     KTFLAIDNADNKTILGFYSLSPASVDYARTPEIIRRRLARHDVPGFRLARLAVDRRLQ
                     GKGIGGQLLLAAGRRCLLASAAVGGVVLVIDAKNERVAGWYASYGAVPLLDAPLSLLL
                     PLATIEAALKKAGKY"
     gene            3013..3237
                     /gene="ssb"
                     /locus_tag="VFC15_14630"
     CDS             3013..3237
                     /gene="ssb"
                     /locus_tag="VFC15_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZM11440.1"
                     /translation="MQKVLLIGNLGRDPEVKYSQQGTAIAQFSVATTERWKDKGGEPQ
                     EHTEWFAVKAFGRRAEVVGEHLRPAQETWL"
     gene            3194..>3574
                     /locus_tag="VFC15_14635"
     CDS             3194..>3574
                     /locus_tag="VFC15_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11441.1"
                     /translation="MSANTSVQLKKPGYKDSAEQQTEIITNQENHLGFILVPSADSAA
                     VAILKPSPPAIVSFTASQSKITMGQTASLKWSTQNVTKAWIEPGIGTVSPNGAHDVSP
                     GQTTTYMLTAKGRGGSTTAKVLLAV"
BASE COUNT          810 a          946 c         1083 g          735 t
ORIGIN      
        1 atctttgccc attcgccagt aagcctggaa caccagcacc ttgcgaaacg gtatgtggtg
       61 cgcgggttgg agtcaggagg cgcggccgga gaacttgggc gatacgtcac tttcacccag
      121 gaagatggtc agccaatcga gtggctgcat ccggttgagg cgatcggagt gaacggctta
      181 catgccgtag tggtggcgcc ggtgctggtg caagtcgaga tgctacgaaa gggacggacg
      241 tacgaactac tgattacgca gcatcgcccg ggtgcgacca acgatggaaa gcggccgcca
      301 atgggaacaa gactcctgtt tcgtggcatt catggtcgct tggatctgga tttgtccggg
      361 aaggacaagg ctggcacggt tctgccaacg ttctattcgc gcgccgggga ggaagtcatg
      421 ataccgaagc ggtttcgctc ggttgtacgc gccatagtcg gggcggttca ttgcgacggc
      481 tgtttccatt gccactatgt cagaccgccg cagaacaaag aggaccagtc gtgcgccgca
      541 gaacgtgtca cagcctgaca tggctgaacg gaaggagtga atagcgatgc atagaaaggt
      601 caagaactac tcactggttt tctgtctgtt tgtgttcctg ttcgtaatta tcggaggcgg
      661 cctccatctg atccaggcgg tgcattcctt gctgcagcta ccggaactcc actgatgatt
      721 tatgccttca atcgcaggtc tagtggggat tgccgaccag agcatgtgcc atcccggctt
      781 gcgggaaatg aggttcgata tggcagcgac agatcggata acccccattg acgaaagtga
      841 agtcgcggtg aacgagagcg caggttcaga atgttccgaa cccacgcgcg cggttcggcg
      901 gcatttgccg gacgagcggc gggcactcac ccatcacttt tctatcgccg ggcaggaggg
      961 atacctaacg gtcggagttt acgaggacgg ggtgccaggc gagatcttta tcaccatggc
     1021 gaagcagggc tcgaccatct ccgagctgat ggatgccttc gcaacggtgg tctcgcttgc
     1081 cctgcagcac ggcgtgccat tgcgtgtgct ttgcgacaaa ctctgccaca tgcgctttga
     1141 gcccagcggt tggtctggaa acccgaagat cggctacgcc aagtcgctta tggactacat
     1201 tgcgcgttgg ctcgaactca ggttcctgaa cagtgaccag ggcgaattgt tcaaagctct
     1261 tccgatgccc gttcagccag cgatcgatgc caatggtact gatccggttg gagcgctcgg
     1321 tgaatttatc cagatgggag atgcgcccgc ctgcaacatc tgcggttcgc tgatggtacg
     1381 gagcggcacc tgctatcgct gcatgacgtg cgggggcgca agcggatgct cgtaatgagt
     1441 ttctcgtgtc aggccgtcgt gtgcagcgcg cagggcaaga aatggcgcta acattacgca
     1501 agttgcgtta cgcatcttgc gtaacgcgag ttgaatccga gaacccagtt gaacgatgag
     1561 gcgcgcagga aagtgagttc taaagatcaa aatccggaga ggatcgaaac gatggtccac
     1621 tccgaccctg agatcatggc gggtacaccg gtgtttcgtg gaacgagaat ccccgtttat
     1681 ctgatcgccg acatgatgga gcagggaacg ccgataaacg aaatcctgga gggctaccct
     1741 tcgctgacga gagacatggt ggagtatgca agtgtctttg cgactctcca cccacgaccg
     1801 gaccgacaga cacggtagcg tacaaagcta cgatgacacg ctcaaaggcg atgttgggag
     1861 gtaacgcaga gatcaaagcc aactttcagg ggggcgacgc taaggccgac ttatgagcaa
     1921 ccaatgcata catgaggttg acgtacacgt ccttatatgt actaattgat tatgtacaga
     1981 gaggagtcag tgccatgccg agagttgcgg ttgaagacaa cagccggatg tccttgcgga
     2041 ttcggcccga ggaaaaagcg ctgctattgc gcgcggtggc gctgaaccac accgacctga
     2101 cggattttgt cattcgccac gccttgcgtg ccgccaaggc agtcatcgag gaagcggatc
     2161 atgtgcagct ctccgcgagg gatagcgtgc gggttctgga gttgttggag aatcctcccg
     2221 cgccgagccg gaaactactg gcagccgccc gggcgttgcc aaagcggtca tgatcgtgac
     2281 ggcgtggcac gaagagtcga tcaacaagac acatgatcgt gaagcgttcg actgcggcga
     2341 agaagcgctg aatgagttct tgcgccgtta tgcgcgcaag agccacgact tgggcggggc
     2401 aaaaacgttt cttgcaattg acaatgccga caacaaaacc atccttggct tctatagtct
     2461 gagccccgct tcggttgact atgcccggac gccggaaatc atacggcgca ggctggcacg
     2521 ccatgacgtt ccaggtttcc gcttggcacg cctcgctgtg gataggaggc tccaagggaa
     2581 gggaattggc ggacaacttc tgctcgcggc gggcagacgc tgcttgttgg cctcggcggc
     2641 ggttggcggc gtcgttcttg tcatcgatgc caagaatgaa agggtggcag gatggtatgc
     2701 cagctatggt gctgtaccgc tgctggacgc gcccttatca ctgctgctgc cgctcgcgac
     2761 aattgaggcc gcgttgaaaa aggcagggaa atactgacac ggaaaagccg tcaggccccg
     2821 acgcatggga caagaatgcg tatttgcagg ctatgtctag ttttcgcagt tcgtgaaatg
     2881 cacggtcttt cctgatactt ccacctgatc agggcagcat gcctcgggtc agtgccgctg
     2941 tttggttgtc aaatacttcg cctcaccact ctcgcaatca atcttacatt ctcaataggg
     3001 aggctaaacg ccatgcagaa ggttctgctg atcggaaatc tcggccgtga ccctgaggtg
     3061 aagtacagcc aacagggtac ggccatcgca cagttctccg tcgccaccac ggaacgatgg
     3121 aaggataagg gcggcgagcc gcaagaacac acggagtggt ttgccgtaaa ggccttcggc
     3181 cgccgtgcgg aggttgtcgg cgaacacctc cgtccagctc aagaaacctg gttataagga
     3241 ttctgcggaa cagcaaacgg aaatcatcac caaccaggaa aaccatcttg gcttcattct
     3301 ggttccgtcg gcagattcgg cagcggttgc catactgaag ccatcccctc cggcgattgt
     3361 gtcattcact gccagccagt caaagatcac gatggggcag accgccagcc tcaaatggtc
     3421 cacccaaaat gtaaccaagg cttggatcga accgggtata ggcacagttt cgcccaacgg
     3481 ggcgcacgac gtctcgccgg gtcaaactac cacttacatg ttgacagcaa aagggcgcgg
     3541 cgggagtacg accgccaagg tattgctcgc ggta
//
LOCUS       DASRKK010000611         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_543679, whole genome shotgun sequence.
ACCESSION   DASRKK010000611 DASRKK010000000
VERSION     DASRKK010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543679"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..760
                     /locus_tag="VFC15_14640"
                     /pseudo
     CDS             1..760
                     /locus_tag="VFC15_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1460..2941
                     /gene="istA"
                     /locus_tag="VFC15_14645"
     CDS             1460..2941
                     /gene="istA"
                     /locus_tag="VFC15_14645"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM11442.1"
                     /translation="MDDWQVIRQRRIGHAEHVKQISRDTGFAINTIRKYSRSSSPPKR
                     TGAPTRTPLMAVYENDVDELLKRESKITSLRIAQLLREKYPSFVLGERAARVYVSRRR
                     ALLHPKEVFIRQVYVAGDQVQYDFKDIRAVIDGNEVDLHMFTARLSYSTAWFARCYHT
                     EDRPALLDGILRASVEFGGLTRDAVFDNATTAVDKVGRGRQRTMNNVFAAFTGSLGIS
                     MQFAAPAKGNEKGGVEGLHGYIEDNFFRPLRSAPSLEAINDELLTLSRRDRDKDTVDG
                     QTIAQRLQTERLALRPLPNVLPRACVAETVRVTKFAEIRYKTNRYSVPGRYVGRPATV
                     EIFADVLRIVVDGELVAKHSRLFGRNEASLDPMHILEALKKKHRAVERAEVFNNERFP
                     QELRDYLKRLVKRDRDTAGKQFLRVMELLIAHRISAILKAVTTAAQLGVDDPAAIELL
                     VRQDSSTTVPPLSTDALPDAAKILVTQPRLDGYAIAGLKETAA"
     gene            2938..3684
                     /gene="istB"
                     /locus_tag="VFC15_14650"
     CDS             2938..3684
                     /gene="istB"
                     /locus_tag="VFC15_14650"
                     /inference="COORDINATES: protein motif:HMM:NF038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM11443.1"
                     /translation="MTNFALLDEHLRALRLPAILANYRRIAGDDAAKLSFLQELVALE
                     VDRRHENGVRARIAAARFPVIKTIESFDFSLQAQLPKAKLLALLDGRFVSDNINPILV
                     GPTGVGKTHVLTAIGLAACTAGYRVLFTTAAELCMNLITAKREDRLKQRLANYERYQL
                     ILIDELGYVPFEKEATDLLYQVIAQRYERASIAVTTNLAFTDWTQVFPDPMTASVVVD
                     RLVHHSMIFEFSGESHRLRTRKGGRPPKGA"
BASE COUNT          892 a         1069 c         1030 g          762 t
ORIGIN      
        1 tcgcaagaac gatcgcagtg acgcacagat tctcgcgcga ctggcacgct tcgatccgca
       61 gctgctcgaa cccattcgtc accgtactgc gccgatgcaa gccgaccttg cgaccatccg
      121 cgctcgcgag acccttgtca cagcacggac caaatgcgtc aacgcgatgc gtggtttggt
      181 gaagtccatg ggcgggcggc tacccaagtg cagcacggag agcttctgca aacgcgtgag
      241 ctccgacgtc ccggcggagc tcgaggacgc attgcggccg ctactcacgg caattcaaac
      301 cctcaacgaa cagatccgct gctgtgacaa caagatcgag acgctcgcaa cggaaatcta
      361 tccgcagacg gcgttgccgc gccaggtggg cggcgttgga gcacttacag cgttggcatt
      421 catcctcacg atcgccgatg gagcgcgatt cgcaaagagc cgcgacatcg gtccttattt
      481 gggattggtg cctcggcaag atgattcggg cgaccacagc tcacagttgc gaatcaccaa
      541 ggctggtgac ccatacctgc gacgactgtt ggttgggagc gcacagtaca tcatagggcc
      601 gttcggaccc gacagcgatc tacgcagaca cggcgaaaag ctgatgcaac ggggcggcaa
      661 gaacgccaag aagcgcgcta tcgtcgcagt cgcgcgcaaa ctcgccgtgc tcttgcaccg
      721 actgtggatc accggcgaga tttacgagcc gctgcgcaac tcgaatcgtc aggcgtcaat
      781 caaagccgca taagttggac cgatgagttt ttcgcgctgc cgccggtccc gaggcgggcg
      841 ttagcggatc aacagcaatc ccagtaatcc agtaacttcc cacggagatc ggagatcaac
      901 cgcgatcgta gcaacgaccc ctcaatgaac cgccgcgttc gggtgactgc gggctagact
      961 ttgatcctta gagatcgtca ttgctagatg gcagcaccca cctttccgag gaacctcaac
     1021 aggcaccgag ccccaaactg ggctcattaa gagtgcggat ggaagcaagg cgaaaggtgt
     1081 ggcggaatgt tggagacggg ctactttaac gggccatgcc tctgggggct tgacaagcac
     1141 cggccttctc atggaagtct aggagcctgt aggtcttcga caaacacgtt cgccaaatgt
     1201 cctgtgacga cgtttgtttt ggcggctcat acgttgttcg gacctcaaat cagggccgaa
     1261 tcgcactctc acgcttattt gcaaccttcg tgaatggaac cgaatcggcc aaatgactaa
     1321 taactggcaa cggcacgatc ttcctgaccc cggcggcacg attatcctga cccctccggc
     1381 agactaagag gccctccctc cgaagctagc tgtaacaagc aagccacctc ggagggaggg
     1441 ccccggaggg ttgattactt tggacgattg gcaagtcatc cgccagcgcc gtatcggtca
     1501 cgcggaacac gtcaagcaaa tctcacgcga caccggattt gcaattaaca ctattcgtaa
     1561 atactcgcgc tcatcgtcgc ctcctaagcg cacaggcgcg ccgacaagaa cgccgttgat
     1621 ggcggtgtat gaaaacgatg tcgatgaatt actgaagcgc gaatcaaaaa taacctcact
     1681 tcggattgca caacttctac gcgagaagta tccatcgttc gtgcttggag agcgcgctgc
     1741 acgggtttac gtatcacgcc gacgagcact attacatccc aaagaagtgt tcattcgtca
     1801 agtgtacgtg gcgggtgatc aagtgcaata cgatttcaaa gatataaggg cagttatcga
     1861 cggcaacgaa gtcgatctgc atatgttcac agcgcgtcta agttactcga ctgcatggtt
     1921 cgcgcgttgc tatcacaccg aagatcgacc ggcgctcttg gacggcatct tgcgcgcgag
     1981 cgttgaattc ggtggattga cgcgcgatgc tgtcttcgac aacgcgacaa ctgctgttga
     2041 taaggtcggc cgcggaagac aacggactat gaataacgta ttcgctgctt tcaccggtag
     2101 cctcggtata agcatgcagt ttgctgcgcc agcgaaaggc aacgagaagg gcggcgttga
     2161 gggactacac ggctacatcg aagacaactt ctttcgaccg ttacgcagcg cgccatctct
     2221 cgaagcaatc aacgacgagt tgctgacatt gagccgtcgt gatcgcgaca aggacaccgt
     2281 cgatggccaa acgatcgctc agcgccttca aacggaacgg ctcgcactgc gtccactgcc
     2341 aaacgttttg ccacgggcgt gcgtcgccga aactgtgcgt gtcaccaaat tcgctgaaat
     2401 acgctacaag acaaatcgtt actcagttcc agggcgctat gttggccgac cagccaccgt
     2461 cgagatcttt gcagatgtcc tgcgaatcgt cgttgatggc gagctcgtag ccaagcattc
     2521 acggctcttt gggcgaaacg aggccagtct tgatccgatg cacatcctcg aagcgctcaa
     2581 gaagaagcac cgtgccgtag agcgtgccga ggtgttcaac aacgagcgat tcccgcaaga
     2641 gctccgcgac tacctcaagc gattggtcaa acgtgatcgt gatactgccg gcaagcagtt
     2701 tctgcgagtc atggagctac tcatagccca tcgaataagc gcgattctga aagcagtgac
     2761 gactgcggcg caacttggcg tcgacgatcc cgcagcgatt gaactgctcg ttcgtcagga
     2821 ttcatcgacg acagtcccgc cacttagcac tgacgcgtta cctgatgcag cgaagatact
     2881 ggtaacacag ccacgcctag atggctatgc gattgccggc cttaaggaga cggcagcatg
     2941 acaaatttcg cgctacttga tgagcatctt cgtgccctcc gtcttccagc gatactcgcg
     3001 aactatcgtc gcattgccgg cgacgacgca gcgaagctga gctttctgca agaactcgtt
     3061 gccctagaag tcgatcggcg acatgagaac ggtgtgcgcg ctcgcatcgc ggcggcgcgc
     3121 tttccggtga tcaagacaat tgagtcattc gacttctctc tgcaggcgca gttgccaaaa
     3181 gcgaagctgc tcgcgctgtt ggatggccgg tttgtttccg acaacattaa tccaattctg
     3241 gtcggtccga caggcgttgg taagacgcac gtattgacag ctatcggact cgcagcgtgt
     3301 actgctggat atcgtgttct gttcacaaca gcagccgaac tctgcatgaa cctaattact
     3361 gctaaacgtg aggatcgact aaagcaacgg ctagccaact acgaacgcta tcaactaatt
     3421 ttgatcgacg aattaggata tgttccattt gaaaaagagg ccaccgattt gctttaccaa
     3481 gttattgcgc aacgctatga acgcgcaagc attgctgtaa cgacgaacct ggcatttaca
     3541 gactggaccc aagtgttccc cgacccgatg accgccagcg tcgttgtaga ccgactcgtc
     3601 caccacagca tgatcttcga gttctccggt gagagccatc gcttgagaac acggaaggga
     3661 gggagaccgc cgaagggcgc gtaaccggcc cgccggggtc attctaatcg tgccgggggg
     3721 gtcagggtaa tccgaccgtt accaataccg cca
//
LOCUS       DASRKK010000612        23653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_740014, whole genome shotgun sequence.
ACCESSION   DASRKK010000612 DASRKK010000000
VERSION     DASRKK010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740014"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..633)
                     /locus_tag="VFC15_14655"
     CDS             complement(154..633)
                     /locus_tag="VFC15_14655"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZM11444.1"
                     /translation="MLRKQNLLRPIQLLGIAVLALTFMGADVDARFNKLGHQLMCMCG
                     CNQVLLECNHVGCAYSERMRGELTASLERGDNDSLALQSFVQKYGNTVLSAPTTTGFN
                     RVAWIMPFAVFAIASAMTVWLVRLWKSRPVAQTVAHPNLGNEELDGLRKKAREETEF"
     gene            complement(633..2669)
                     /locus_tag="VFC15_14660"
     CDS             complement(633..2669)
                     /locus_tag="VFC15_14660"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZM11445.1"
                     /translation="MPQIGSFTLLLALALAVYSFVAGALALYRKDDRLGETARRAGIA
                     CWAAVTIAAIALVLAAFGNDFSVAYILHHSNRDLPIAYKFAALWSGQEGSLLLWAWLL
                     SSYGLVMRLRYKVDPQLTAYASAIVAAVQVFFLLLVNFAAHPFGLVTGTIPPDGNGLN
                     PLLQYPEMVIHPPMLYLGYVGVTAPFAFALGALIMKYPGEKWIHITRRWTMVAWLFLT
                     CGVFLGAHWAYAVLGWGGYWGWDPVENASILPWLTMTAFLHSVMMQEKRGMMKMWNMW
                     LIFTTFLLAIFGTFLTRSGVVSSVHAFAQSGIGIWFVWFLATAFATCVFFFVRNRETL
                     KSEHRLDSLISRESSFLFNNLVLLVACFAVLWGTLFPVLSEWVTGSKVTVGPPFFNRV
                     NVPIGLFLVFLTAVGPLLAWRQTSWDSLRRNFLWPTIIAAGTAIALIAGGMKPWQDSA
                     YFYSLMAISLAALVMATIVGEFWRGGRVIARQQHINILEGMVQITRRNTRRYGGYIVH
                     FGIILIVIGFAGVPFNQDKEQEMGFGDKMQIGAYTLVCQSFTQDDTPNYGSEWAVLDV
                     YKNGKKIDTMFPERRFYKASEQTSTIVANRSRPNEDLYLVYTGKNPDTGKPIIKAHVN
                     PLVMWIWIGVVVVGFGTVLALIPNTKPGKINATQKERDETEAAVKRGAVEVGGD"
     gene            2984..3475
                     /locus_tag="VFC15_14665"
     CDS             2984..3475
                     /locus_tag="VFC15_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM11446.1"
                     /translation="MTQEQQLREQLAKILDWGEAHADFRTAVQDFPLDLRGEVPPGFS
                     HSAWELLEHLRIALSDIVEFTRNARHKSPPWPKGYWPPLPTPPQDEAWDQCVQSILDS
                     LEDMRALVLDRSRSLLDPLPRGQGQTLLREALLVADHNAYHLGQMVQLRKALRAWPKD
                     PAQ"
     gene            complement(3660..6095)
                     /gene="priA"
                     /locus_tag="VFC15_14670"
     CDS             complement(3660..6095)
                     /gene="priA"
                     /locus_tag="VFC15_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZM11447.1"
                     /translation="MPRFCDVAVPVPLDAAFTYSIPEELSEPCVGGRVIVPFREKRLC
                     GIVTELHDREPKFEAKPVLQVLDTVSALTPELMQLGRWIAHYYIAPIGEVLRTMLPLA
                     AEFRRVIGYRITDKGLEALHAASTIGSSLRSRQEPEQQMLEYAVLNRLADGEMMRESS
                     LRAAAGAPRLVLQTLLRKKWIAREDLSGVRDASRNIQIATLKAAEGKLNANQQRIVDY
                     LGLQPEQSATAAALRELAVPRTTLQTLVRRGIVELHDEPAGFRMSGLKPRKLEFLFNA
                     AQKAALDQINAAVDKRQFLPMLLHGVTGSGKTAVYLSAMQAMLAKGRSAILLVPEIGL
                     TPAMAADLHQIFGDEVAILHSALTDDERAEQWKRIRSGESHIVVGTRSAVFAPVPDLA
                     LIVVDEEHDHSYKQDETPRYHGRDVAVMRAKMCNAVAVLGSATPSLETYYNATQGRYK
                     LIELAERIEQRPLPEVEILDMREEFQHTKKEEVLSHKLVEEIGECMARNEQAMILLNR
                     RGFSSFVLCRSCGDTVQCRNCAIAMTYHKREHRLICHYCGFVRPAPKTCPKCGSEYVQ
                     YLGTGSEKLEQILHGLFPQARIGRLDRDTVRGRDDLERILSALHAGEIDLLVGTQMIA
                     KGHDIPNVTLVGVVGSDAALSFPDFRAAERTFQLLTQVAGRAGRGENPGKVVLQTFYP
                     DHYAIQFAAAHDYRGFYEKESRFRSWMHYPPFNSVSNVVVRSAKLDEALTWSGILGKW
                     FEATRLEGVRVMGPAAAAIVRLKTEYRYHFLLKSASRERMNAALRSMIEHAVAQKIPR
                     TNLVVDVDALSLT"
     gene            complement(6102..6980)
                     /locus_tag="VFC15_14675"
     CDS             complement(6102..6980)
                     /locus_tag="VFC15_14675"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZM11448.1"
                     /translation="MTLDPASKSAVRERLKFYRELGIGPLYRREVAGDAPTRDLSEGL
                     VEVLAAVGDELAVQQEQTAGEAYLPTAQNPRMEKSRGEDREALLRVILDDIGPNCQRC
                     KLATLGRKQIVFGTGDPHAELMFIGEGPGADEDQQGLPFVGRAGQLLNNMIVAMGLKR
                     EEVYIANIVKCRPPGNRTPERDECDTCSPFLMRQIRVVRPKVIVALGATAAKNLLGMN
                     DSMAALRGRFYDFSPKGSLTDAPFDCKLAVTYHPAFLLRDPRQKKETWKDLQMVMKFL
                     GLQPPTRATTPDAEGS"
     gene            complement(6977..8224)
                     /gene="coaBC"
                     /locus_tag="VFC15_14680"
     CDS             complement(6977..8224)
                     /gene="coaBC"
                     /locus_tag="VFC15_14680"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZM11449.1"
                     /translation="MMKIALGVTGGIAAYKAAEIVRLLQDRGVQVQVIMTDAAQEFVR
                     PLTFAALSGEKVITGLFSGDEAEPNLDSAVEHIAVAQLIDALLVAPATADALAKFAQG
                     IANDFLSTLYLATKAPVIVAPAMNVNMWEHPATRANLEILRQRGVQIVEPDSGYLACG
                     MTGAGRLAEPEHIVAATLERLGIAQDLAGETVLVTAGPTHEPVDPVRYIGNRSSGKMG
                     YALAEAALRRGAHVVLVSGPTALIPPSAAETIFVETAQQMRTAVLDRWEKAGIIIMAA
                     AVADYHVKNVSEQKIKRSGPIDLRLEPNPDILADLGSLRHATGKSTPLLIGFAAETTS
                     LLENARAKLTRKRVDAIVLNDVSRPDIGFNSDRNQVTLVTAQDAIAIPEASKLDVAQT
                     ILDAAVRLRQQHATLVPSPVRSQ"
     gene            complement(8278..8514)
                     /gene="rpoZ"
                     /locus_tag="VFC15_14685"
     CDS             complement(8278..8514)
                     /gene="rpoZ"
                     /locus_tag="VFC15_14685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZM11450.1"
                     /translation="MNLADGFDSKYRYILVAARRARQLQNGAPPVVEPHSAKACRIAE
                     QEINAGKVKWEMSKTPAQVASEILSKAVGGESDE"
     gene            complement(8527..9267)
                     /gene="gmk"
                     /locus_tag="VFC15_14690"
     CDS             complement(8527..9267)
                     /gene="gmk"
                     /locus_tag="VFC15_14690"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZM11451.1"
                     /translation="MSGIVFIISAPSGSGKTTLTAELRRVVPNLEFSISYTTRLPRGS
                     EQSGREYFFIAREEFTKMIGEDQFVEYAEVFDNYYGTARSFLREAFARGNDVILDIDV
                     QGASQVKQRMPEAVSIFILPPSRQELESRLRRRTLAENAERSKLTGEEMAAAKVEAIL
                     QRRLQEASREIENYSQYDYILVNEKVEAAIDVLQAIVKAERVKRSPGPRDAEEQRVLA
                     TAELAERRHMMEKVQTILATFDFSGTRS"
     gene            complement(9264..10154)
                     /locus_tag="VFC15_14695"
     CDS             complement(9264..10154)
                     /locus_tag="VFC15_14695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZM11452.1"
                     /translation="MKIRSMTGYAQVKTHVNDQLTLTVSLKSVNHRFLDLHLRIPSEL
                     DQVEMKVRRVLKERLHRGHIEVTLSAERTGGAEFAVNRELVGGYLNAFRQAAEEFGVS
                     AEPDLNAILKLPGALSAAGSGAAAEELDQQVTAALEQAIERLNHMREEEGHGAGVELR
                     NCMQHLSKATREVEGLRGKVLKAYHEKVHGRMQELIGAHADPDRILQEAAMLAERSDI
                     QEELVRMKNHIEHFLSHLDSGGEVGKKLDFLLQEMNREANTLLSKTTGVAGEALRITE
                     LGLAMKSDIEKAREQVQNLE"
     gene            complement(10162..11991)
                     /locus_tag="VFC15_14700"
     CDS             complement(10162..11991)
                     /locus_tag="VFC15_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZM11453.1"
                     /translation="MRQLKRLLHYVKPYWLQLLASVLSMALVGLLDAFRLLLIGPIFD
                     HVLNPGTQPRELQLLPTTRWAMQLNWFVPERFHNVWTMVAFALVAATVLKGVFDYLGT
                     YLVNYAGFGMITDLRNELYTATLRRSVAFFQKHSTGTLLSALVNDIERVQYAMSTALA
                     EFLQQFFTLVFTAVVVVLLGGKLAWVLLLFVPVILVSARRLGQQVRRTTRKGQDKLAE
                     IQNILHETITGVRIVKAFSMEAWETGRFRDAAKRLFRANLRSVSAAAISSPMMDIFGA
                     IAIALLILMGREYIKHHVFTEGIFLAFIVAVFKLYEPVRKFAQLNNNFQQALGASSAI
                     FKFLDAQDDVREKTGAVKMPPFHQGIRFDHVYFSYKQGDDHREVLHDINLDVAPGEVL
                     AIVGSSGSGKTTMVHLLPRFFDVSHGRLLIDGHDVRDLTVASLRSEIAIVTQDTILFN
                     DTVRNNIAYGQPTVPMEKVEEAARAALAHDFIMGLPNGYDSKIGERGLRLSGGERQRI
                     SIARAILKNAPILILDEATSALDTESESLVQSALQNLMTGRTVFVIAHRLSTVRRADR
                     ILVLENGMITDEGTHERLMTHTGTYRRLYDLQFVDVEISNGSE"
     gene            complement(12635..13582)
                     /gene="rapZ"
                     /locus_tag="VFC15_14705"
     CDS             complement(12635..13582)
                     /gene="rapZ"
                     /locus_tag="VFC15_14705"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZM11454.1"
                     /translation="MASKKRATSRAANKKSLSKKRVALPALARDQHELVIITGISGAG
                     KLSALKTFEDLGYYAVDNLPFGLLLNFADLVRDSTEIERAALVVDIREGARIDKLPQL
                     LQSLRQSLKTTVLFLEANEESLLRRYSETRRPHPLGQKTSVKASLLSERRRLLPVREI
                     ADLVVDTTKFNVHELRSLLTERFQSAKREQNILISCVSFGFRQGVPEDADLMFDVRFL
                     PNPHFIPKFRPLTGRNPQVARYILSFPQTKEFIARISELLVYLIPHYIREGKSYLTIA
                     FGCTGGKHRSVLITEEVKKRLAKAGYNVKVVHRDSPAAE"
     gene            complement(13667..14341)
                     /locus_tag="VFC15_14710"
     CDS             complement(13667..14341)
                     /locus_tag="VFC15_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM11455.1"
                     /translation="MATTSIRVLIADDHPVVRVGLRNMLQADSLVKVVGEARDGVEAL
                     ELARKLRPDILLLDLAMPHVAGMDALREMTSGNLNTRTIVLTGLIDNRQILEALQLGA
                     RGVVLKDAVVEHLSQCIRAVMQGQYWLEGRPVQNLVQVLRDLTAQTAPQPRKTFGLTA
                     REMEVVTLITEGCTNKDIAKQFGISEETVKRHLTNIFNKLGVGNRLELALFALNHNLL
                     PPADGQ"
     gene            complement(14368..14760)
                     /locus_tag="VFC15_14715"
     CDS             complement(14368..14760)
                     /locus_tag="VFC15_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZM11456.1"
                     /translation="MPVNETTLASQVFDQLQQAMVCDPAGFTELYRDYLTDAWQALRK
                     LSEAVQQKQADEVRARAHRPKGSSQVLGAREVAQCASALEEMGRNDDVQGAGVVLEQA
                     GRALQQVQAELSNRLGLAVVPADQTAAW"
     gene            complement(14905..15675)
                     /locus_tag="VFC15_14720"
     CDS             complement(14905..15675)
                     /locus_tag="VFC15_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZM11457.1"
                     /translation="MGHVQTELKADVLRITLDRPEKKNALSNAMYTALAEAVEHAEAN
                     PDVRVMLLDGNGDSFTAGNDLEDFVANPWKGQTVPPALRFIAAVVGAKKPIIAAIQGM
                     AVGIGVTILLHCDLVYAADNAKLVMPFVNLGIVPEAGSTVLLPALMGHQRAAELLMLA
                     APVTAQRAYELGLVNAVVAPEALLPAAMEAAQKLAEKPAGALRATKALLRKAGQAELE
                     RAIREEVQVIAERLGSPETREALAAFLQKRKPDFSQFR"
     gene            15885..16643
                     /locus_tag="VFC15_14725"
     CDS             15885..16643
                     /locus_tag="VFC15_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11458.1"
                     /translation="MKRRDQFASLAIMFLATAGAAIGAGPDAVTPPCGAARSLTSEQV
                     SQRLATNNAERARQLRNFEARRQYTLDYTGFPSSRSAEMNVKVSYRAPGTKEFAVLAE
                     SGSKLILTRVFRKLLESEQESSGDEKSRTDVALTADNYHFALRGCEADAGRDLYVMDV
                     QPLRDTKFLYRGTIWIDAQDFAVTRIDAEPARNPSFWTKKSQIHHRYQKIGEFYLPSL
                     NETVTDVRLGGKAVLTIRYQDYKLNQSGRNAGGN"
     gene            16764..17738
                     /locus_tag="VFC15_14730"
     CDS             16764..17738
                     /locus_tag="VFC15_14730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM11459.1"
                     /translation="MVSRELASDLRLRPSARPSRLALFLAFFAIYVVWGSTYLAIRYA
                     VETIPPLVVAGVRHIVAGLVLLAWAYARGHRPTLQQWRASLVLGTLYFAIGHGSLHWA
                     ETVVPSGLAALLIASEPIWIAGMAAVVSHGERLTGKTVVGGLLGIAGVTLLVGGGTAS
                     GNRAVLIGSIAILVGTLSWSVGVFYSRSASLPRDSVARAGMPEIMGAAFLLLVAGFTG
                     EFSHFQIATVTARSAWALLYLITFGSIVAFTAYTWLLDHCSPTLVSTHTYANPIIAVL
                     LGWLWAGEVISTRVLEAGLLTLFAVFLISRGTRQSPQLKAEIEESEVA"
     gene            17781..18413
                     /locus_tag="VFC15_14735"
     CDS             17781..18413
                     /locus_tag="VFC15_14735"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HZM11460.1"
                     /translation="MTRAKADWEREFELSGGVLCLDFANTVLKRNQPGRAKDELENYG
                     RLVGFAKQTKLFSPAKADRLRKRASVSSRAVRQVLPAAIKLREAIYRVFSALAAGKPV
                     ASKDVKLVEDFALEAGKHRRLMPESHGDYRWQWKPEETESPGQMLWPIALSAAELLTS
                     DRLRAVRECAADDCAWLFLDESRNHSRRWCDMTVCGNRQKARRHYHLIQR"
     gene            complement(18424..19428)
                     /locus_tag="VFC15_14740"
     CDS             complement(18424..19428)
                     /locus_tag="VFC15_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZM11461.1"
                     /translation="MAAFQWLAEEAVPDALHDSTAPSQDPDIGLLDAYSRAVVGAVAR
                     VSPAVVHIQVQQKNSRQGGSGSGFVFTPDGFMLTNSHVVHGAAEISVTTEEGDRFPAE
                     LVGDDPDTDLAVIRGRSSAPSVPLGSSRQLAVGQLVIAIGNPLGFQHSVTAGVVSALG
                     RTMRSRSGRLIDGVVQTDAALNPGNSGGPLVDSQGRVVGVNTATIMGAQGICFAIGAD
                     TAEFVASRLIRDGRIRRSYVGVMGQGVPLHRRIIRYYDLPSDSGLLVESVVAGSPAER
                     AGLRERDVIVRFDGRSISGVDDLHRLLTAERSGQSAEVEVVRNTELRKLFIVPQSQRD
                     "
     gene            complement(19510..20514)
                     /locus_tag="VFC15_14745"
     CDS             complement(19510..20514)
                     /locus_tag="VFC15_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11462.1"
                     /translation="MPIAGFRVLLGVSCLLVFVGTISLAQNTGNQQLPSNPQPQATPL
                     SPAPPQTPRTAPSTSNAPSSTAQAPAAADAQTAIVIPAAAERHYHAGRAAEKDKDSAA
                     AIAEYRAAIKEYPDYVDARYRLANLLMDHQGYDEAVAELRQVVMLRPNDANARNDLGF
                     ALKKSGDAKGAAAEYLQAIRLNPKLAVAQNNLANLLYANKQYAAAITHYQAALQLQPN
                     NADARMNLATALDVIGKTDDAIVEYKEALRLRPNDANIHFNLAIAYQNKKDLMAAIAE
                     LKSASKLAPDWVSPRLMLVNMLKNSDPKTALDECMVADGLSHDAKLHDQCLELQKKTQ
                     "
     gene            complement(20572..20808)
                     /locus_tag="VFC15_14750"
     CDS             complement(20572..20808)
                     /locus_tag="VFC15_14750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZM11463.1"
                     /translation="MLTPAFENWQVLRVCLHTDVRNQRSRAAIERVGGKFEGILRAHR
                     MAADYIARDSARYSIVAAEWPDVKRKLHQLLDQA"
     gene            complement(20875..21093)
                     /locus_tag="VFC15_14755"
     CDS             complement(20875..21093)
                     /locus_tag="VFC15_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11464.1"
                     /translation="MLYQRSPVPQGKVEATSYIETALAWQDAGTAVPFATVRVDDGVV
                     IGSTRFFNIEHWAWPENHPRRGHVVDAR"
     gene            complement(21443..22420)
                     /locus_tag="VFC15_14760"
     CDS             complement(21443..22420)
                     /locus_tag="VFC15_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11465.1"
                     /translation="MRIFIGGSLENVPRDKEDCVAFVVALARATVEQGHTLLNGCRSS
                     LDLETAKAARDWLVNNGRQPADQIKSYCLKGAKPVHNIGRVLYSELSDWNMGHPDLKL
                     PEQIDQADLTIFVAGGEGTFWAKNWAFYARKPILGIPRFGGAGETIYEQELTRLRAAI
                     PAVAEEYEMLNQLVTDNFQKYANDTIDLAKRLAIPRSVFAIMTFKPEFADVIDSYQSA
                     CGEFDFKAERTDDPESPNSLERIIPRIEMGIRQSAIVIADVSESSPNVFYELGFAKAL
                     GKDVIVTAKEGTPRPFDIGDVPILYWQSQIELKQKLRACLGTLRHKYGI"
     gene            complement(22681..>23653)
                     /locus_tag="VFC15_14765"
     CDS             complement(22681..>23653)
                     /locus_tag="VFC15_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11466.1"
                     /translation="FLNQMAGPAHQADWGMRIISQQDPNYGPTGYHFGSVWPLFTGWA
                     SIAGYRYHRPLYGYANLMANVQLAHDGSPGRVTEVLSGDYYTPLSTSTPHQIWSSAMV
                     ITPLMRGLLGLEVNALKSSVVLAPHLPADWNDFTIHNVKVGASLLDFTFHRAGDDMTL
                     EVQRHGKDNVQLEFSPALGLRAKVVDATVDGNKVSPTISTNENDQHASISASIGGDTT
                     KIHLHVTGDFGIAYPFAAPADGGPSSNLKIVSEAWNDAHDKLQLQVAGVGGAKYEVPV
                     FNAPSGIAVEGAVIIKTESGLSLEVSFPPGTPGTYTTRAVTLQFPTK"
BASE COUNT         4567 a         7223 c         7030 g         4833 t
ORIGIN      
        1 tcagataacc atagcgcgtc ttggcctcgc cgatctcgac ttcacccggc tgatagaaca
       61 cgtacacgaa cagcgcggcg gcaaacagcg cgcatccaaa caggatgaac ggcgtctcct
      121 gcgcgacgac tacacttgcg atcaatgaat ccatcaaaat tccgtctcct cacgcgcctt
      181 cttgcgaagc ccgtcgagtt cttcgttccc caggttcgga tgcgccacgg tttgcgccac
      241 aggacgcgac ttccatagcc gtaccagcca caccgtcatc gcgctggcga tggcgaacac
      301 cgcgaacggc atgatccacg cgacccggtt aaatccggtc gtggtcggcg ctgacaacac
      361 ggtgttgccg tacttctgca cgaaactctg cagcgccagc gaatcgttgt cgccgcgctc
      421 cagcgacgcc gttagctcgc cgcgcatgcg ttcggagtag gcgcagccca catggttgca
      481 ctccagcagc acctggttgc aaccgcacat gcacataagc tggtgcccga gcttgttgaa
      541 gcgggcatcc acgtcggcgc ccatgaatgt gagagcaagc acggcaatac cgagcaattg
      601 aatcgggcgg agcagattct gcttgcgcaa catcagtctc cgcccacttc caccgcgccc
      661 cgcttcaccg cggcttccgt ctcgtcgcgt tccttctggg tcgcattaat cttccccggc
      721 ttcgtgttcg ggattagcgc cagcaccgtt ccgaatccca ccacgactac gccgatccaa
      781 atccacatca ctaacggatt cacgtgcgcc ttgatgatcg gcttgccggt gtcaggattc
      841 ttgccggtgt atacgaggta cagatcttcg tttggccgcg agcggttggc cacgatggtc
      901 gatgtctgct cgctggcctt gtagaaacgc cgctccggaa acatggtgtc gatcttcttg
      961 ccgttcttgt agacatcgag cacggcccac tcgctgccat agttgggcgt gtcgtcttgc
     1021 gtgaacgact ggcacaccag cgtgtacgcg ccgatctgca tcttgtcgcc gaatcccatc
     1081 tcctgctctt tgtcctggtt gaacggcaca ccggcgaagc caatcacgat caggatgatg
     1141 ccaaagtgca cgatgtatcc accgtagcgc cgggtgttgc gccgggtgat ctgcaccatg
     1201 ccttcgagaa tgttgatgtg ctgctgtcgc gcaatcaccc gtccgccgcg ccagaattcg
     1261 ccaacgatgg tcgccatcac cagcgccgcc agcgagatcg ccatcagcga atagaagtag
     1321 gcactgtcct gccacggctt catgccaccc gcgatcagcg cgatggccgt ccctgcagcg
     1381 ataatcgtcg gccaaaggaa atttcgtcga aggctgtccc acgatgtctg acgccatgcc
     1441 agcagcggcc cgacggcggt caggaacacc aggaacaatc cgatgggtac attcacgcgg
     1501 ttgaagaacg gcggtcccac ggttaccttg ctgcccgtta cccattccga caacaccgga
     1561 aaaagcgtgc cccacagtac cgcgaagcaa gccaccagta gcacgaggtt attgaagagg
     1621 aagctcgact cgcgcgagat cagtgaatcg aggcgatgct cgctcttcag cgtctcgcga
     1681 ttgcgcacga agaagaacac gcaggtcgca aacgcggtag ccaggaacca cacgaaccag
     1741 atgccgatgc ccgactgcgc gaaggcatgc accgaactga ccacgccgct gcgcgtcaga
     1801 aacgttccga agatcgctag caggaacgtg gtgaagatca gccacatgtt ccacatcttc
     1861 atcatgccgc gcttctcctg catcatcacc gaatgcagaa acgcagtcat cgtcaaccat
     1921 ggcaggatcg acgcgttctc caccgggtcc catccccagt agcctcccca tcccagcacc
     1981 gcgtaggccc agtgcgcgcc tagaaaaacg ccgcaagtca gaaacaacca agccaccatg
     2041 gtccagcggc gggtaatgtg gatccacttt tcgccgggat acttcatgat cagcgcaccc
     2101 agcgcgaagg cgaacggcgc ggtcactccg acgtagccga ggtacagcat cggaggatgg
     2161 atcaccatct cgggatactg cagcagcgga tttaacccgt tgccgtccgg cggaatcgtg
     2221 cccgtgacca gaccaaaggg gtgcgcggca aaatttacca gcaggaggaa gaacacctgc
     2281 accgcggcca caatcgccga cgcgtaggcc gtgagctggg gatcgacctt gtagcgtagg
     2341 cgcatcacga ggccatagga cgacagcagc cacgcccaca acaacagcga accttcctgg
     2401 cccgaccaca gtgctgcgaa cttgtacgct atgggcaggt cgcggttgct gtggtgcaga
     2461 atataggcca cggagaaatc gttgccgaag gcggccagca ccagcgcgat ggcggcgata
     2521 gtgaccgcag cccagcaggc gattccggcg cgccgtgcgg tctcgcccag gcggtcgtcc
     2581 ttgcgataca aggccagcgc tccggcaacg aacgaataga cagccagggc cagggcgagc
     2641 agaagagtga aactacctat ctgtggcatg agtgattcag tacctatttt gtttctgtgc
     2701 cggaccaatc tgggccgacg ccatccgtca tgaatgatct cattttactc gcaagccccg
     2761 ttcggtgcgc cggtagccta ttgattcacg gttttcgaat aagtgggagt ttgcccgggc
     2821 gactttatcg agcgcggcgc tattcggcgc tattctgaga ctggaccgga tcactgggat
     2881 ggatggcacg catcgattca gaacccgggg gtcgcgacgg gcggcaacca accccggcct
     2941 cctgctacga tgttctcgcg gccatccggc agcaggagga cgcatgacgc aggaacagca
     3001 gcttcgcgaa caactcgcaa aaatactcga ctggggtgag gcgcatgcgg acttccgcac
     3061 agccgtgcag gatttccccc tcgacctgcg gggcgaggtg ccgcccggct tttcacactc
     3121 tgcatgggaa ttgctggagc acctgcgaat cgcgttgtcg gacatcgttg agttcactcg
     3181 taatgcgcgc cacaaatcgc caccctggcc aaaaggttac tggcctccgt tgccaactcc
     3241 accgcaggat gaagcctggg accagtgcgt gcagtcgatc ctcgactcgc tggaagacat
     3301 gcgcgctctc gtcctggacc gctcgcggtc gctgctcgat cccctgccgc gcggacaggg
     3361 gcaaacgctt ttgcgcgaag ctctgctggt ggccgaccat aacgcctacc acctcgggca
     3421 gatggtgcag cttcgtaagg ccttgcgcgc ctggccgaag gacccggccc agtagcctgc
     3481 tgcaaaatat ccggaagaag gcgtaatctg ccgctatcgg tattccggga aatcagtctg
     3541 gggtgaggag gtcgatcgtg caggaacaac tacaagtcgg taacgcgcgg atgatcggtt
     3601 atgccatcgg gatcgctgcc gcggtcgcgg tcatcctgtg gaagctcgtc atccgataac
     3661 tacgtcagcg acagcgcatc cacatctacc accaagtttg tccgcggaat cttctgcgcg
     3721 acggcgtgct cgatcatcga gcgtaacgcg gcgttcatgc gctcgcggct cgctgacttc
     3781 aacaggaagt gatagcggta ctcggtcttc aggcgcacaa tcgccgctgc cgcgggtccc
     3841 atcacgcgca cgccttccag gcgcgtggct tcaaaccact tgccgagaat tcccgaccag
     3901 gtcagcgctt cgtcgagctt tgcgctacgc accaccacgt tgctgacgga gttgaacggc
     3961 gggtagtgca tccaactgcg aaagcgcgat tccttctcgt agaagccgcg atagtcgtgc
     4021 gccgcggcga actgaatggc gtagtgatcg ggataaaacg tctgcagcac gaccttgccg
     4081 ggattctcgc cgcgaccggc gcgtcccgca acttgcgtga gcaactggaa cgtcctctcg
     4141 gcggcgcgga agtcgggaaa gctcagcgcc gcgtccgatc ccaccacgcc caccaaggtc
     4201 acgttaggaa tgtcgtgtcc cttggcgatc atctgcgtgc ccacgagcaa gtcgatctcc
     4261 ccggcgtgca acgcactcaa gatgcgctcc agatcgtcgc gcccgcgtac cgtgtcgcga
     4321 tcgaggcggc cgatgcgcgc ttgcggaaac agtccgtgga ggatttgctc cagcttttct
     4381 gatcccgtgc ccaggtattg cacatactcg ctgccgcact tgggacacgt cttaggcgca
     4441 ggccgcacga atccgcagta atgacagatg aggcgatgct cgcgcttgtg atacgtcatg
     4501 gcgatggcgc agttgcggca ttgcaccgta tcgccgcagg agcggcatag cacgaacgac
     4561 gagaagccgc ggcggttgag caggatcatc gcctgctcat tgcgcgccat gcactcgcca
     4621 atttcttcga ctaacttatg cgacagcact tcttcttttt tggtgtgctg gaattcctcg
     4681 cgcatgtcga ggatttcgac ttcgggtagc gggcgctgtt cgatgcgctc cgccaattca
     4741 atcaacttgt agcggccctg cgtcgcgttg taataggtct cgagcgaggg cgtggccgag
     4801 cccagcaccg ccaccgcatt gcacatcttc gcgcgcatca ctgccacgtc gcgaccgtga
     4861 tagcgcggcg tttcatcctg cttgtaggag tggtcgtgct cctcatcgac cacgatcagc
     4921 gcgaggtcag ggaccggcgc gaaaaccgcg gaacgtgtgc ctaccacaat gtgggactcg
     4981 ccgctgcgga tgcgcttcca ctgctcggcg cgctcgtcgt cggtgagcgc cgagtgcagg
     5041 atcgcgacct cgtcgccgaa aatctggtgc aggtcggctg ccatcgcggg tgtcagtccg
     5101 atctcgggca ccaacagaat cgccgagcgt cccttggcca gcatggcctg catggccgac
     5161 agatagacag ccgtcttgcc cgagccggtg acgccatgca gcagcatggg aagaaattgc
     5221 cgcttatcga ccgcggcatt gatctggtcg agcgcggcct tttgcgccgc attgaacaga
     5281 aactccagct tgcgcggttt cagccccgac atgcggaagc ccgcgggctc atcgtgcagc
     5341 tcgacgatgc cgcgccgcac gagggtctgc aaagtggtgc gcggcacagc cagctctcgc
     5401 agcgcggctg ccgttgcgct ctgctccggc tgcaggccaa ggtagtcgac gatgcgctgc
     5461 tggttagcgt tgagcttgcc ttcggcagcc ttcagagttg cgatctggat gttgcggctg
     5521 gcgtcgcgaa ctccgctgag atcttcacgg gcaatccact tcttgcggag aagggtctgc
     5581 agcaccaagc gcggagcccc ggccgccgca cgcagcgagg actcacgcat catttcgcca
     5641 tcggccagcc ggttgagcac ggcatactcc agcatctgct gttcgggctc ctgccgtgag
     5701 cgcagcgacg agccgatggt cgaggcggcg tgcagcgctt cgagtccctt gtcagtgatg
     5761 cggtatccga tgacgcggcg aaattcggca gccagtggca gcatggtgcg cagcacttcg
     5821 ccgatgggag cgatgtagta gtgggcaatc cagcgcccga gttgcatcag ttcgggcgtg
     5881 agagccgaga ctgtgtcgag cacttgcagc accggcttgg cttcgaactt gggctcgcga
     5941 tcatgcagtt cggtgacgat gccgcacaac cgcttctcgc gaaagggaac gataacgcgt
     6001 ccgccgacgc acggctcgga caactcttcg ggaatggagt aagtgaaggc ggcgtccagc
     6061 ggaaccggca cggccacatc gcagaagcgg ggcatggcgg gctagctgcc ttcggcgtcc
     6121 ggcgtggtcg cgcgtgttgg cggctgcagt cccaggaact tcatcaccat ctgcaggtcc
     6181 ttccacgttt cttttttctg gcgcggatcg cgcaacagaa acgcgggatg ataggtgacc
     6241 gccaacttgc aatcgaaggg cgcatccgtc agcgagcctt tgggcgagaa atcgtagaag
     6301 cgtccgcgca gagctgccat ggagtcgttc atgccaagca gatttttggc ggcggtggcg
     6361 cctagtgcca cgatcacctt ggggcgcacc acgcgaatct gccgcatcag gaacggagag
     6421 caggtgtcgc actcatcgcg ttccggggtg cgattccccg gaggacggca cttcacaatg
     6481 ttggcgatat acacctcttc gcgtttcagt cccatcgcca cgatcatgtt gttgagcagt
     6541 tggccggcgc gccccacgaa cggcagtcct tgctggtctt cgtccgcgcc cgggccttcg
     6601 ccgatgaaca tcagctccgc gtgcggatcg ccggtgccga agacgatctg cttgcggccg
     6661 agtgtggcca gtttgcagcg ctggcagttg gggccgatgt cgtcgaggat aacgcgcagc
     6721 agcgcctcgc gatcttcgcc tctcgacttt tccatccggg gattttgcgc tgtaggaaga
     6781 taagcttcgc ctgcggtctg ttcctgctgc accgcaagtt catcgcccac tgcggcgaga
     6841 acctcgacaa ggccctcact caagtcccgt gtcggagcgt cgccagctac ctcgcggcga
     6901 tacagcgggc caatacccag ctcgcgataa aacttaaggc gttcgcgcac tgcggatttg
     6961 ctggcgggat cgagagtcat tggctacgga ccggtgaagg caccagtgta gcgtgttgct
     7021 ggcggagccg gaccgcggca tcgagaattg tctgtgcaac gtcgagcttg ctggcctcgg
     7081 gaatcgcaat cgcgtcctgc gcggtcacca acgtgacctg gttgcggtcg gagttgaagc
     7141 cgatgtccgg gcgcgacaca tcgttgagca caatcgcatc cacccgcttc ctggtcagct
     7201 tggcgcgcgc gttctccaga aggctagtgg tttcggcggc gaagccgatc aacagtggcg
     7261 tgcttttgcc ggtagcgtgc cgcaggctgc ccagatcggc gaggatgtcg gggttgggct
     7321 cgagacgtag gtctatcgga ccgctgcgct tgatcttttg ctccgacacg ttcttcacgt
     7381 gatagtcggc aaccgcagcc gccataatga tgattccggc cttctcccag cgatcgagca
     7441 cggcggtgcg catctgctgc gcggtctcga cgaagatggt ctccgctgcc gatggaggaa
     7501 tcagcgctgt cggtccgctg accagcacca cgtgagctcc gcgtcgcagc gcagcttccg
     7561 ccagcgcata gcccatcttg ccgctggagc ggttgccaat gtagcgcacg ggatcgaccg
     7621 gctcgtgtgt cggcccggcg gtgaccagca cggtctcgcc ggcaaggtcc tgcgcgatgc
     7681 cgagccgttc gagcgtagca gccacgatgt gttcgggttc ggcgagccgt cccgcgccgg
     7741 tcatgccgca agccaggtag ccgctgtcgg gctcgacgat ctgcacaccg cgctgccgca
     7801 ggatttcgag attcgcccgc gtggcgggat gctcccacat attgacgttc atcgcgggcg
     7861 ccacgatcac cggcgctttg gttgcgagat aaagcgtcga aagaaaatcg ttggcgatgc
     7921 cttgcgcgaa cttcgccagc gcgtcggctg tggctggcgc aacgagcagc gcgtcaatca
     7981 actgcgctac agcgatgtgt tccaccgcgg aatccaggtt tggctcggct tcatcgccgc
     8041 tgaacaaccc ggtaatgacc ttttcgccgg agagcgccgc gaaggtgagc ggccgcacga
     8101 actcctgggc cgcgtcggtc atgatgacct gcacctgcac gccgcggtcc tgcaacagcc
     8161 gcacaatttc ggcagccttg taggcggcaa tgcctccggt cactcccaag gcgattttca
     8221 tcatgtcctc agctctcccc cgcatgcggt gggatgcaat tcttagatgc ggacgaccta
     8281 ctcgtcgctc tccccgccga ccgccttcga caaaatctcg ctggcaactt gcgcaggagt
     8341 cttgctcatc tcccacttta ccttgcccgc attgatttct tgttcggcga tgcggcacgc
     8401 tttggcggag tgcggttcga ccaccggggg cgcaccattt tgcagttggc gggcccgtcg
     8461 ggccgcgacc aggatgtagc gatacttgct gtcgaatcca tcagccagat tcatctgtcg
     8521 ctccctttag ctgcgagtac cggaaaaatc aaatgtcgcc aggatggtct gcaccttctc
     8581 catcatatgc cgacgttcag ccagttcggc ggtggccagc acgcgctgct cttcggcatc
     8641 tcgcgggccg ggcgaacgct tcacgcgctc ggccttcaca atcgcctgta gaacgtcgat
     8701 ggcggcctcc accttctcat tcaccagaat atagtcatac tgggaataat tctcaatctc
     8761 gcgcgacgct tcctgcaatc gccgctgcag gatggcctcg acttttgccg cggccatttc
     8821 ctcgcccgtc agtttgctgc gctcggcgtt ctccgccagg gtccgtcggc gcaagcggct
     8881 ctccaactcc tgtcgcgatg gcggaagaat aaagatgctc acagcctcgg gcatccgctg
     8941 ttttacttgc gacgcaccct gcacatcgat gtcgaggatc acgtcgttgc ctcgcgcgaa
     9001 ggcctcgcgc agaaaactgc gagcggtgcc gtaatagttg tcgaagactt cggcgtattc
     9061 cacaaactgg tcttcgccga tcatcttcgt gaattcctcg cgggcgatga aaaaatactc
     9121 gcggccgctt tgctcgctgc cgcgcggcag ccgggtggtg tagctgatgg aaaattccag
     9181 gttgggcacg acgcggcgca gttccgctgt cagcgtggtt ttgcccgagc ctgaaggggc
     9241 cgagatgatg aacacgattc cactcattcg aggttttgta cctgttcgcg ggctttctcg
     9301 atgtccgact tcatcgccag gcccaattcg gtgatacgca acgcctcgcc ggcgacgccc
     9361 gtggtcttgg acagcagcgt gttggcttca cgattcattt cctgcagcag gaagtccagc
     9421 ttcttgccga cttcgccacc gctatcgagg tgcgacagga aatgctcgat gtggttcttc
     9481 atccgcacca gttcttcctg gatgtcgctg cgctccgcca gcatcgcggc ctcctgcagg
     9541 atgcggtccg gatcggcatg cgccccgatc agctcctgca tgcggccatg aactttctcg
     9601 tgataggcct tcagcacttt gccgcgcagg ccttcgacct ctcttgtcgc cttcgacaaa
     9661 tgctgcatgc aattgcgcaa ctccacgcct gcgccgtggc cctcttcttc ccgcatgtga
     9721 ttcagccgtt cgatggcctg ctccagcgcg gcggttacct gctgatcgag ttcttcagcg
     9781 gcggcgcccg atccggccgc gctcagtgcg cccggcaatt tcaggatggc attgaggtcg
     9841 ggctcggcac tgacgccaaa ctcttcggcg gcttgccgaa atgcgttaag gtacccgccc
     9901 accagttcgc gattaaccgc gaactctgct ccacccgtcc gctcggcact cagcgtgact
     9961 tcgatgtgtc cgcgatgcag ccgctccttc agcactcggc gtaccttcat ctcgacttgg
    10021 tccagctccg aagggatccg caaatgcaga tcgaggaagc gatggtttac cgacttaagg
    10081 ctgacggtga gcgtgagctg atcgttcaca tgcgttttga cctgcgcgta ccccgtcatc
    10141 gaccggatct tcatgcgtgg ctcactcgct cccattggaa atctcgacat cgacgaactg
    10201 cagatcatac aggcggcggt aggtgcctgt atgtgtcatc agacgctcgt gtgtgccttc
    10261 gtcggtaatc atgccgtttt ccaggaccag gatgcggtcg gccctgcgta ccgtgctaag
    10321 ccggtgcgcg atgacaaata ccgtgcgtcc ggtcatcagg ttctgcaatg ccgattgcac
    10381 cagcgactcg ctctcggtat cgagtgccga ggtcgcttca tcgagaatca gtatgggcgc
    10441 attcttcagg atggcccgcg cgatggaaat ccgctggcgt tcgccaccgc ttagccgcag
    10501 tccgcgctca ccgatcttgc tgtcatagcc atttggaagt cccatgatga agtcgtgtgc
    10561 taatgccgca cgcgccgctt cttcgacctt ttccatgggg actgtcggct ggccgtaagc
    10621 gatgttgttg cgcacggtgt cgttgaacag gatcgtgtcc tgcgtgacga tggcgatctc
    10681 ggaccgcaac gaagccacag tgagatcgcg cacgtcgtgg ccgtcgatga gcagccgccc
    10741 gtggctcaca tcgaagaaac gtggcagcag atgcaccatc gtagtcttgc ccgatccgct
    10801 ggaacctacg atggccagca cttctcccgg cgcgacgtcg aggttgatat cgtgcagcac
    10861 ttcgcgatgg tcgtcgccct gcttgtagct gaaatacacg tgatcgaagc ggattccctg
    10921 gtggaagggc ggcatcttga ctgcgcccgt cttctcccgc acgtcatcct gggcgtcaag
    10981 aaatttgaag atcgccgacg acgcccccag cgcctgctgg aagttgttat tcagctgcgc
    11041 aaactttcgc accggctcat agagcttgaa cacggccacg atgaaggcca gaaaaatgcc
    11101 ttccgtgaac acgtgatgct tgatgtactc gcgccccatc aggatcagca gcgcaatcgc
    11161 gatagcgccg aaaatatcca tcatcggtga actgatggcg gcggcgctga ccgatcgcag
    11221 attggcccgg aacagccgct tggcggcatc gcggaatcgg cccgtctccc aggcctccat
    11281 tgagaacgcc ttcacgatgc gcacgcccgt gatggtttcg tgcagaatgt tctggatctc
    11341 cgccagtttg tcctgcccct tgcgcgtggt tctgcgtacc tgctgaccca accggcgggc
    11401 agacacgaga atgaccggaa cgaacagcaa cagcacccag gccagcttcc cgcccagcag
    11461 cacaaccacc acagcggtga agacgagagt gaagaactgc tgcaggaatt ccgccagtgc
    11521 cgtggacatg gcgtactgca ctcgctcgat atcattcacc agcgccgaaa gcagcgtgcc
    11581 ggtcgagtgc ttctggaaga acgccaccga gcggcgcagg gtcgcggtat aaagttcgtt
    11641 gcgcaggtcg gtgatcatgc cgaatccggc gtagttcacc aggtaggttc cgagataatc
    11701 gaagacgcct ttcaaaaccg tggcggcgac cagcgcaaag gccaccatcg tccacacgtt
    11761 atggaagcgc tcgggcacaa accagttcaa ctgcattgcc cagcgcgtgg tgggcaggag
    11821 ttgcagctcg cgcggttggg ttccgggatt cagcacgtgg tcgaagatgg gcccgatcag
    11881 cagcaaccga aaggcgtcga gcagtcccac cagcgccatc gacaacacgg aagccagcaa
    11941 ctgcaaccag tacggtttga cgtagtgcag caggcgcttc agttgacgca taggcaccgc
    12001 ggtttccgaa gcagtatgcc gggcccactc gtattggagg gaggtatgtg caaactgtga
    12061 ttttagcgga actgtagggc gggcggatat gggtagatga ggcgatgcaa tgtcagggtg
    12121 gcccgctcaa gctcagctta agcatgagtt cacctgagcc accgacacat aagtgtggtc
    12181 ctcacggtgc caagcatgca tcatcatagc gccctggctg agctgactcc gctggggagg
    12241 agattttacc gcgcgcgacc atgtacccgg ttcttatccg ggggcacttc aatcaagccc
    12301 taacgaccat caggcttgct gcaagtcatc ttattgggat cgcgccagct tcttgtttcg
    12361 agcactctct gctcccgacg ttccggctcg tgctcgcgag ggaagcgatt cagatacggt
    12421 tcatgaactt caggttcagt tttgttggag gttgcagtga actgcgcaaa caatttgaac
    12481 caccccataa acgacctctc gggaataggc taatgcgaag gcgtaattgc ggtctgttca
    12541 gaattgctca cggcatgaat caaggccgcc ggccaagctg atcttgctct gagggcaggg
    12601 gtgccactct tggacactta tcgcggctac cgacctactc cgccgccggc gaatcccgat
    12661 ggaccacctt cacgttgtag cccgccttgg ccagccgctt cttgacttct tcggtaatca
    12721 gcaccgagcg atgcttgcca ccggtgcagc caaacgcgat ggtgaggtaa cttttgcctt
    12781 cgcggatgta gtgcgggatc agatacacca gcagctccga aatgcgggcg ataaactcct
    12841 tggtctgcgg gaacgacagg atgtatcggg ccacttgcgg attccgcccg gtcaacggcc
    12901 ggaacttggg aatgaagtgc gggttgggca ggaagcgcac gtcgaacatc aggtcggcat
    12961 cctccggcac gccctggcgg aagccgaagc tcacacagga aatcaggatg ttctgctcgc
    13021 gcttggcgct ctggaagcgc tcagtcaaca gactgcgcaa ctcgtgaaca ttgaactttg
    13081 ttgtatcaac caccaggtcg gcaatctcac gcaccggcag caggcgtcgc cgctccgaga
    13141 gcagcgaggc cttgaccgaa gtcttttgcc ccaacgggtg cggacgccgc gtctcgctgt
    13201 agcggcgcag cagcgactct tcgttggctt cgagaaacag caccgtggtc ttcagcgact
    13261 ggcgcagcga ttgcaacagt tgtggcagct tgtcgatccg cgcgccctcg cgaatatcga
    13321 cgaccagcgc cgcgcgctcg atctccgtcg agtcacgcac caggtcggca aaattcaaga
    13381 gcaggccgaa cggcagattg tcgaccgcgt agtagcccag gtcttcgaag gtttttaagg
    13441 ctgagagctt tccggcgccg ctgatgccgg tgatgatcac cagctcgtgc tggtcccgcg
    13501 ccagtgccgg cagcgcgacc cgcttcttcg acaacgactt tttgttcgcg gctcgcgaag
    13561 tagcgcgttt cttcgaggcc atggtcttgg gagattcctg tgggcagaga gtatcagact
    13621 tcgcgtcccg cgtcagccgt ttcggttgaa caggacgcgg ctttggctat tgaccgtccg
    13681 ccggcggcag caaattgtga ttgagcgcaa acagcgccag ctccagccgg tttcccacgc
    13741 ccaacttatt aaagatgttg gtgaggtggc gcttgaccgt ttcttcgctg atgccaaact
    13801 gcttagcgat gtctttgttg gtgcaacctt cggtgatgag cgtaaccact tccatttcgc
    13861 gcgcggtcaa cccgaacgtc ttgcggggct ggggagcggt ttgagctgtc aggtcgcgca
    13921 acacctgcac gaggttttgc actggccgtc cttccagcca gtattgcccc tgcatcacgg
    13981 cgcgaatgca ctgcgacagg tgttcgacca ccgcgtcctt cagcactaca ccacgagctc
    14041 ccaattgcag cgcttccaga atctgacgat tgtcgatcag ccccgtcagt acgatggtgc
    14101 gggtgttgag gttgcccgag gtcatctcgc gcagcgcgtc cataccggcg acgtgcggca
    14161 tggccaggtc cagcagcagg atgtcggggc gcaatttccg cgccagttcc agcgcttcta
    14221 caccgtcgcg cgcttccccc accaccttga cgagggaatc cgcctgcaac atgttacgca
    14281 ggccaactcg aaccacgggg tgatcgtcag caatcaggac gcgaattgaa gtcgttgcca
    14341 ccatagcact cgagctgtga gatggttcta ccacgccgcc gtctggtccg ccggaaccac
    14401 cgcaaggccc agacggtttg agagttcggc ctgcacctgc tgcaatgccc gcccggcctg
    14461 ctccagcaca acgccggcac cctggacatc gtcattgcgt cccatttcct cgagggcgga
    14521 tgcgcattgt gccacctcgc gcgcgcccag aacctggctg ctgcctttcg ggcgatgcgc
    14581 cctcgcgcga acttcgtccg cctgcttctg ctgcaccgct tcactcagct ttcgcagtgc
    14641 ttgccacgcg tcggtcaggt agtcccggta aagttctgtg aatccggcag gatcgcaaac
    14701 catggcttgc tgaagctggt cgaatacttg cgacgcgagc gtcgtctcgt tcacaggcat
    14761 caggcgctgc ctcacgtcag acttagtacc ccataggtaa acatcctcag caacttacgt
    14821 caagcgcgga atgcccgtgg ttgccaggag ccacgtccgg cattccgtaa gaaagatgcg
    14881 cgagttcatg gttatcgaaa cgtgttatcg aaactgtgaa aaatctggtt tgcgtttctg
    14941 gagaaacgcc gccagtgctt cgcgcgtctc cggagaaccc aaccgctcgg caatgacctg
    15001 cacttcttcg cggatcgcgc gctccagttc cgcctggccc gctttgcgca gcagagcctt
    15061 ggtcgcccgc agcgctcccg ctggcttttc cgcgagcttt tgcgctgcct ccatcgcagc
    15121 cggcaacagc gcttcgggtg caactaccgc attcaccagc cccaactcat aagcacgctg
    15181 tgccgtaacc ggagcggcca gcataagcaa ttccgccgct cgctgatggc ccatcagtgc
    15241 gggcaatagc acggtcgagc ctgcctccgg cacgatccca aggttgacga acggcatgac
    15301 gagcttggcg ttgtccgcgg catagaccag gtcgcaatgc agcaggatgg tgacgccgat
    15361 gcctaccgcc atcccttgga tcgccgcgat aatcggcttt ttcgccccca ccaccgccgc
    15421 gatgaaccgc aacgccggtg gcactgtctg ccccttccag gggttggcta caaagtcttc
    15481 gaggtcgttg cccgcagtga acgagtcgcc gttgccgtcc agcagcatca ccctgacatc
    15541 gggatttgcc tcagcgtgtt ccaccgcctc cgccagcgcg gtgtacatcg cgttcgacag
    15601 ggcgttcttc ttttcgggac ggtccagcgt aatgcgcagc acgtccgctt tcagttcagt
    15661 ttgcacatgg cccatgttcc tacccctgcc gcgccagaat tgaactgcac ccacggcggc
    15721 acggccaacg gaattgtact ggaagaattt gcgggaagcg gtgatagtgc aaaagtgaac
    15781 tcctagggtt cccctcttga tgtaagctaa gacggttcca tgacttccga gccttctatc
    15841 accggattgc tcgccctcgt taccgctctg cgcgtcgtgt gctcatgaag cgacgcgacc
    15901 agtttgcatc cctcgcgatc atgttccttg ctactgcggg tgccgccatt ggcgctggtc
    15961 ccgacgcggt gacaccaccc tgcggggccg cccgcagtct caccagcgag caggtttccc
    16021 agcgcctggc aacgaacaac gccgagcggg cgcgacagtt gcgcaacttt gaagcgcgtc
    16081 gccagtacac gctcgactac accggttttc cctcgtctcg cagtgccgag atgaacgtga
    16141 aggtctccta ccgcgcaccc gggaccaagg agtttgccgt gctcgccgag tctggttcaa
    16201 agctcatcct gactcgcgtg tttcgcaaat tgctggagtc ggagcaggag tcttcgggcg
    16261 acgagaaaag ccgcactgat gtggcgctga ccgccgacaa ctatcacttc gctctgcgag
    16321 gatgcgaagc ggacgctggc cgggatcttt acgtgatgga cgtgcaaccg ctgcgcgaca
    16381 ccaagttcct ctatcgcgga accatctgga tcgatgccca ggattttgcc gtgactcgca
    16441 tcgacgccga acccgccagg aacccctcgt tctggacgaa aaagtcgcag atccatcacc
    16501 gctaccagaa gattggcgag ttctacctgc cctccctgaa cgagaccgtg accgacgtcc
    16561 gcctgggcgg aaaggccgtg ctcaccatcc gttaccagga ctacaagctg aatcaatccg
    16621 ggcgaaatgc gggcggaaac taaccgagat tcgcagggcg aatcagccca ttgttaactc
    16681 ctgaattgcg gcccacaggt caggccggga actgcagtta gaagttagtg tgctacagtc
    16741 tataaccagc gaggactatt tcagtggtta gtcgcgagct ggcatcggat ctgcgcttgc
    16801 ggccttcggc ccgtcccagc cgcctggccc tgttcctggc cttcttcgcg atctacgtcg
    16861 tctggggatc cacctacttg gcaatccgtt acgcggtgga gaccattccg ccgctggtag
    16921 tcgccggtgt tcgccacatc gtcgcggggc tggtgctgct ggcttgggcc tacgcgcgcg
    16981 gccaccgtcc aacgctacaa caatggcgtg ccagcctggt gctgggtacg ctctatttcg
    17041 ccatcggcca cggcagcctg cattgggccg agacggtggt gccttccggg ctggcggcgc
    17101 tactgatcgc cagcgagccc atctggatcg ccgggatggc tgccgtggtg tcacacggcg
    17161 agcgccttac cggcaagacc gtagttggtg gtctgctggg aatcgccggt gtgactctgc
    17221 tcgtgggcgg tgggaccgcc tcggggaacc gcgcagtact catcggatcg atcgccatcc
    17281 tggtggggac gctttcgtgg tcggtgggtg tcttttactc gcgcagcgca tcgttgccac
    17341 gcgattccgt ggcgcgggca ggaatgccgg agatcatggg agccgcgttt ttgctgctgg
    17401 tcgcaggttt cactggcgag ttttcgcact tccagattgc gaccgtgacg gcgcggtcgg
    17461 cgtgggcgct gctctacctg atcacctttg gatcgatcgt cgccttcacc gcctacactt
    17521 ggctgctgga tcattgttcg ccgacgctgg tgtcaaccca cacctacgca aatccgatca
    17581 tcgcggtgtt gctgggatgg ctctgggctg gggaagtcat cagcacgcgg gtgctggagg
    17641 cgggactact gacgctgttt gcggtgtttt tgatcagccg cggcacccgt caatccccgc
    17701 aactgaaagc ggaaatcgag gaatctgagg tcgcatgagc taacctctgt tcgcgagaat
    17761 agtagttata agaggaaaac atgacgagag cgaaagccga ttgggagcgg gaattcgaac
    17821 tcagcggggg cgttttatgc ctcgatttcg ccaacactgt gctgaaacgc aatcagccgg
    17881 ggagagcaaa ggacgagttg gagaactatg gccgcctggt cgggttcgcg aaacaaacca
    17941 aactgttctc cccggccaag gccgaccggc tgcgaaagcg ggcgtccgta tcttcccggg
    18001 ccgtgcgcca ggtgttgcct gcggcgatca agttgcggga agcgatctat cgcgttttct
    18061 ccgcgctggc tgccggcaag ccagttgcgt ccaaagacgt aaaactagtc gaggatttcg
    18121 ccctcgaggc ggggaagcat cggcggttga tgccggagtc gcatggcgat tatcgctggc
    18181 agtggaagcc ggaggagacg gaaagtcccg ggcaaatgct gtggccaatc gcgctgtcgg
    18241 cggcggaatt gctgacctcg gaccggctgc gagcggtgcg agaatgcgcc gccgacgatt
    18301 gcgcatggct gttcctcgac gagagccgca accatagccg tcgctggtgc gatatgacag
    18361 tctgcggcaa tcgccagaag gcgcggcggc attaccacct gatccagagg tagccctcac
    18421 gtttcagtcg cgctgggact ggggaacaat gaacaacttg cgcaactccg tatttcgcac
    18481 gacttcgacc tccgcggact gaccgctgcg ttccgcggtc agcaggcggt gcaggtcgtc
    18541 cacgccggag atggatcgcc cgtcgaagcg cacgatgaca tcgcgctcgc gcaatccggc
    18601 gcgctcagcg ggggagcccg cgaccacgct ttccaccagc aacccgctat ccgaaggcag
    18661 gtcgtaatag cgaatgatgc ggcggtgcag cggcacgccc tgtcccatga cgccgacata
    18721 gctgcggcgg atgcgtccat cgcgaatcag gcgcgatgcc acgaattcag ccgtgtccgc
    18781 gccaatggcg aagcagatgc cttgggcgcc catgatggtt gcagtattca cgccgaccac
    18841 gcgcccttgc gaatcaacca gcggtccgcc cgagttaccg gggttcaacg cggcatcggt
    18901 ctgcaccacg ccgtcgataa ggcgaccgga gcgcgaccgc atggtgcggc ccagggcgct
    18961 gacgactccc gcggtcacgc tatgctggaa gcccagcgga ttgccgatgg cgatcaccag
    19021 ttgtcccacg gcgagttggc gcgagctacc cagcggaact gaaggcgcag agctgcgtcc
    19081 gcgaatcaca gcgagatcag tgtcgggatc gtcgcccacc agttcggcgg gaaaacgatc
    19141 gccctcctcc gtggtcacag agatctccgc cgcgccatgt acgacgtggc tgttagtcag
    19201 catgaagccg tcgggggtaa acacgaagcc ggaaccggat ccgccctggc ggctattttt
    19261 ctgttgcacc tggatatgca ccaccgcggg gctaacgcgc gcgaccgcgc ccaccacggc
    19321 ccgcgagtag gcgtcgagca atccgatgtc gggatcttgc gatggagcgg ttgaatcatg
    19381 cagcgcatca ggaaccgctt cttccgcgag ccattgaaaa gcagccataa ttcctcgtga
    19441 aggtgtggat gccggcagag ggcattgcga ttcgcgcgcg gaaacagcgg ggtagaaaag
    19501 cgccccaggt tattgcgttt tcttctgcaa ttcgaggcac tgatcgtgga gtttggcgtc
    19561 gtgcgagagt ccgtcggcaa ccatgcactc atcaagcgca gtcttgggat cggaattctt
    19621 caacatattt accagcatca agcgcggaga aacccaatct ggcgcgagct ttgatgccga
    19681 tttcagttcg gcaatggctg ccatcaagtc tttcttgttc tggtaggcaa tggccagatt
    19741 gaagtggatg ttcgcatcgt tggggcgcag gcgcagcgcc tctttgtatt cgacaatggc
    19801 gtcgtcggtc ttgccgatca cgtcaagcgc ggtggcgagg ttcatgcgcg cgtcggcgtt
    19861 gttgggctgc aactgcaacg cagcctgata atgcgtgatg gcggcagcat attgcttgtt
    19921 ggcatagaga aggttcgcca ggttattttg cgcgaccgcc aatttgggat taaggcggat
    19981 ggcctgcaag tattccgcag ccgcgccttt ggcgtcgcca ctcttcttca gggcgaaacc
    20041 cagatcgttg cgggcgttgg cgtcattggg ccgcaacatc accacctggc gcaattccgc
    20101 gacggcttcg tcgtatccct ggtggtccat cagcaggttg gcgaggcgat aacgcgcgtc
    20161 cacgtagtcg ggatactctt taatggcggc gcgatactcc gcgatggctg ctgcggaatc
    20221 tttgtccttc tcagcggcgc ggccggcatg ataatgccgc tcagcagcgg cgggaatgac
    20281 gattgctgtt tgagcatcgg cggctgccgg ggcctgcgcc gtagacgacg gtgcattaga
    20341 agtcgaaggc gcggtgcgtg gcgtctgagg aggagcgggc gacaaaggag tagcttgcgg
    20401 ctgcggattc gatggaagct gctgattgcc cgtgttctgc gcgagcgaaa tagttccgac
    20461 aaaaaccagc agacaggata cgccgagcag cacccgaaat cccgcgattg gcacagaaac
    20521 tctcatgaac gcgtaagacg agtatatcgt ttcaaggcga cggcgcgccg gtcatgcttg
    20581 gtccagcagt tggtgcagtt ttcgcttcac gtccggccac tctgcggcca cgatcgaata
    20641 gcgagccgag tcgcgagcga tgtaatcagc cgccatgcga tgggcgcgga gaattccttc
    20701 gaactttccg cctacgcgtt cgatcgcggc gcgcgaacgc tggttacgaa cgtccgtgtg
    20761 caggcagacg cgaagcacct gccaattttc gaacgcaggg gtcagcatta gcagcttggc
    20821 ctcggtgttg gccgcggtgc gaatcgccga acggctcagc catgtgtaac cgatctagcg
    20881 ggcgtcgaca acgtgcccgc gccgaggatg gttctccggc cacgcccagt gctcaatgtt
    20941 aaagaagcgg gtggagccga tcacaacacc gtcatctacg cgaactgtcg cgaacggcac
    21001 cgcggtgccg gcgtcctgcc aggccaaggc agtctcgatg tagctcgtgg cttcgacctt
    21061 accttgggga acgggactcc gctgatagag caagggtcga tttccgaggc ggctacgagc
    21121 ccgtcaacgt gccggtggtc gagtggctca agacgaatgt gcctgcctgt caacactgga
    21181 gcttcgattt tcacggcttc cagtatccaa tgagtcccta tggggctgca atgcaagtca
    21241 tggattcaca actggattct cagttcgcga gcgctgggtg cgccgctcat tcgcgaattc
    21301 tagggctgag gaggagcagc gtgatcgagc acgaccacca gtgtaagcag aaattacgcc
    21361 ttattggttt agtccacaaa tctgatctag ccgtaccagt tgccctacgc aagcaaagaa
    21421 aactcggttg gataaaccgt cgtcatatcc catacttatg tcttagcgtg ccaagacatg
    21481 ccctaagttt ctgtttcagt tctatctgtg attgccaata gaggataggt acatcaccta
    21541 tgtcaaaggg ccgcggcgta ccttctttcg cagtcactat aacatccttt ccaagagcct
    21601 ttgcaaatcc cagttcatag aagacattcg gactagactc gctaacgtca gcaataacaa
    21661 tagcgctttg acgaattccc atttctatgc ggggtatgat gcgctccaga gagttgggag
    21721 actccggatc gtcagtcctc tccgccttaa agtcaaattc accacatgcc gattgataag
    21781 aatcaattac atcagcaaac tccggcttaa acgtcatgat cgcgaacacg cttcgcggga
    21841 tagctagccg cttggccagg tcaattgtgt cattcgcgta cttttgaaaa ttgtccgtga
    21901 caagctgatt gagcatttca tattcttcag ccacagcagg gatagctgct cgaagacgcg
    21961 tcaactcctg ctcgtaaatg gtttcgccag ccccaccaaa tcgcggaatt cctaagatgg
    22021 gcttccgggc gtaaaacgcc caattttttg cccagaacgt accctcacct ccagcaacga
    22081 agatggtcag gtcagcttgg tcaatctgtt ccggaagctt taaatcaggg tggcccatgt
    22141 tccagtctga gagctctgag tagagaactc ttccgatgtt atgaactggc ttcgcgcctt
    22201 tcagacaata actcttgatt tggtcagcag gctgtcttcc attattcacg agccagtccc
    22261 gcgctgcctt tgcggtctcc agatctaacg agctacggca accatttagc aaagtgtggc
    22321 cttgttccac agtcgcgcga gccaaggcta caacaaaggc gacgcaatct tccttatctc
    22381 tgggtacgtt ctccagcgag cctccgatga aaatgcgcat gatcctggtc cacccccatt
    22441 gtgttggtct gcttcgtctg cgagcaacgc ttatcttgag ccatgtggcg tgcgctttgg
    22501 gcgcgatgtg gtaaacgtat catggcttgt caatattgcc gcccccaaaa cagacctaac
    22561 tctgggccgc cgtagtggcc gactccccag ttcaaagatc ggataatgcg ccgagatggt
    22621 ctgccgccgc cgctgtgcca gccattccgc gcttgttgga acacggcgca cctcgtgctt
    22681 tcattttgta ggaaactgaa gcgtgaccgc gcgggtggta taggtaccgg gcgtccctgg
    22741 cggaaaagac acttccagac ttaaccctga ctctgtcttg atgatcaccg caccctcgac
    22801 tgcgatgccg ctgggtgcgt tgaaaactgg gacctcatac ttcgccccgc ccacgcccgc
    22861 gacttgcagt tgcagcttgt cgtgcgcgtc gttccacgcc tcggagacga tcttcaagtt
    22921 tgaactggga cccccgtcag cgggtgcagc aaagggatag gcgatgccga agtctccggt
    22981 aacgtgcagg tgaattttcg tggtgtcgcc gcctatcgat gccgatatcg aggcgtgctg
    23041 atcgttttcg ttggtcgaga tcgtcggcga aaccttgttg ccatcgaccg tggcatcgac
    23101 aactttcgcg cgcaagccca gggccggaga gaattccagt tggacattgt ctttgccgtg
    23161 tcgctgcacc tctagggtca tgtcgtcgcc ggcgcggtgg aaggtgaagt caagcaaact
    23221 ggctccgacc ttcacgttgt ggatggtgaa gtcgttccag tcggcaggaa ggtgcggggc
    23281 cagcacaacg ctggatttca gggcgttaac ttccaggccc agcagtccgc gcatcagcgg
    23341 tgtaatgacc atggccgacg accagatctg gtgtggcgtg ctggtggaaa gcggagtgta
    23401 gtagtcgcct gacagcacct cggtcacgcg cccgggcgat ccgtcgtgcg cgagttgcac
    23461 gttggccatg aggttggcgt agccgtagag cgggcgatga tagcggtatc cggcgatgga
    23521 ggcccatccg gtaaacagcg gccagacgct gccaaagtga tatcccgtgg gcccgtagtt
    23581 ggggtcttgt tgcgagatga tgcgcatgcc ccagtcggct tgatgcgcgg gccccgccat
    23641 ctggttgagg aag
//
LOCUS       DASRKK010000613        16102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1228186, whole genome shotgun sequence.
ACCESSION   DASRKK010000613 DASRKK010000000
VERSION     DASRKK010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228186"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFC15_14770"
     CDS             complement(<1..1576)
                     /locus_tag="VFC15_14770"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HZM11467.1"
                     /translation="MSRFTVVPIGLIIFLSSLASGQAPQQNRIVRPVDNRSVVQLQGT
                     MHPLARAAFDKGPTSPTQTMQGMKLIFQPTAEQQAALEQLLIEQQDPASSNYHKWLTP
                     EEFADRFGVSAQDINTLAAWLTSQGFSVDQTARSRTWIAFSGIAAQVNAAFQTSIHNY
                     LVNGKMHYAASSEPSIPAAFAGVVTGIGALHDFAPQARSSKAKPRVTSSVTGNHFVAP
                     GDFATIYDLQALYASGINGTGQTIAIMGQTDLSTDSNPGRNGTPGAVVNGQQQQYDVV
                     TFRGLAGLPPLTSSNFQIVVVPGTTDPGLVTAELDEANLDVEWSGAIAPNANLIYVIE
                     NANSQGAFGALQYAVDQNLAPILSISYGLCEPQVDSATQSSMTTAGQLANAQGQTIVT
                     PSGDSGAADCDANSPATQGLAVDFPASLPTVTAMGGTTFSADSTNSSAPTAATQYWAG
                     STNDTNPSALAYIPEVAWNDTPAPTTSGTAFAATGGGVSTFSNGVKPPWQTGLGVPND
                     NARDVPDISFASSSGHD"
     gene            complement(1754..2887)
                     /locus_tag="VFC15_14775"
     CDS             complement(1754..2887)
                     /locus_tag="VFC15_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM11468.1"
                     /translation="MSRRLSLILVLALLTVGAVAIHAQTPPVVQSPEVQADGRVTFRF
                     FDPGAKEVQLNFEGAPKLLPMTRDDSGLWSITLGPLDPDLYGYIFIADGVYLLDPNNS
                     SLKPNLLQPQNVVEVSGPTPQLWDVAEVPHGVIHHHFFKSKVVGDQRDFYVYTPPGFD
                     PKAKTKYPVFYLLHGFSDSADGWTAVGKANVILDNLIAQSKVKPMIVVMPLGYGTPEM
                     IKLKQAAWNHPDVRQQNFDGFRKSLLTEVMPMVEGAYPVSTKREDRAIAGLSMGGSES
                     LLTGLNNIDKFAYVGSFSAGGLSDDYEPAFPTLDAKQASQLRTLWIACGTSDRLNDDN
                     RKFKAWLKSKDIAFTDIETPGAHTWMVWRRNLINFAPLLFTSK"
     gene            complement(3113..4690)
                     /locus_tag="VFC15_14780"
     CDS             complement(3113..4690)
                     /locus_tag="VFC15_14780"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZM11469.1"
                     /translation="MISWRALSRRFPWLDIPALLLIVIVGVLSFKYEDFKDDFPRLIT
                     WQLRTYQWITDLGLGKPNAESVTPIEIDDTTFYDFFGNETRDEITDRRFLAQLVDAAT
                     QAQAAVIALDINLDEARFDKQRDAPESLNADDQALLDAIRRAQAVKIPVVLTFGLRRD
                     QVPVPQVFDNQVVYEGKGISYEFPEDESPPRPYWIARFGFDHPGDDFRKVPLVAVGHD
                     DQGNSFDFYSFALQITDSFEHRGNKTALEDKALVDRLTRHEFVYTSFMPVSAFVDPRL
                     QVDRDHTGSLSAVEVFCSPGFEKKQEKKQNWNMQICDQQNRTRMQLALELISGHIVLI
                     GGNRHAYKGEHDEDDYLDDHDSPVGPIRGMYYQANYVEGLLSGRILFKVNRWAAALID
                     VGLAILMLWIVSLRRGTGTRLLVMLLLLVPAALAYFAATYFHRCLDFMFPLVLLLLHP
                     AIEGYVRPHSQEVAHEEIAPHDAVVDRHADFAGESERRVAPAGSSSARPDHEQPTEEA
                     GGESEESGLDEELDGRR"
     gene            complement(4690..5913)
                     /locus_tag="VFC15_14785"
     CDS             complement(4690..5913)
                     /locus_tag="VFC15_14785"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HZM11470.1"
                     /translation="MLLRSISRFALAALLLCASFTAAQERCGAGTDLMVQALERVQPS
                     STPSQLRDAVELLKHATSECISLGDAWYYRSLLERKLGNTKLADYSLEKARQNNSEAL
                     QQQLDPFVLSTNPAVKPAGPVREKWALIVGIGKFQDKAIPTLRYTTADATGFADTLKN
                     AGVGRFKPQNVAVLTDYQATTKNIREKLNWLARTAQPDDLVVIYIATHGSSRDLDTAG
                     VNYIITADTEISPKRHAAGDTTGDTDKDIDHDALFATALPMVDVTNTVASRVHAQRVA
                     VFLDTCFSGAAAGSGGTKTVASAMGLKSVSGATLSRMSEGAGRVILAASQENQESLES
                     ASLQHGYFTYYLLEALKQSKGMDPIGKVYLFVREQVATRAKQQQIPVMAQSDQGDLIV
                     LGVPTTSAAAASGGP"
     gene            complement(5916..7322)
                     /locus_tag="VFC15_14790"
     CDS             complement(5916..7322)
                     /locus_tag="VFC15_14790"
                     /inference="COORDINATES: protein motif:HMM:NF018281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11471.1"
                     /translation="MLRFPQVMRSLPVRLPAAALLLVTYGSGLAFAQGFGSSKKKITL
                     LRKLPPTAHLNGTAIDVQVTAHNIQGDVATDLRNMLETELLKDDRRLRSEDKHPDSLV
                     ICTITEYAPPQAQTSSHSTYVPGSKKPQQETVTRYSGILKVAYQAKDAHSGRTLDSDN
                     VTAKYDDEFNQYGSTSGGVTGTLTHTWDKLKHGKLEGDKPPTAAELRDVLLSNVVSQI
                     ASRLVNTEEPVEVLLARGKLDDADKLAEANLWTRNLEALETMTPFPSKEDDAYRLYNI
                     GVAYEALAYQSEDAKAAQKYLQEAAINYGKAIDDKPEEKYFLQPQNRIDTAIAHYKKL
                     REQPSAPAVADSGGKSGSDDSAKSAKSGRSSGGSTSTTTKSASAKSTSSSSGTTAAKS
                     PAPAAKKATGPSLNNDQVIQMVKAGVDEDNIIDTIHSAGAVNFDLSVDGQVKLAQNGV
                     KGKVLTAMKTRARQLSSH"
     gene            complement(7442..8407)
                     /locus_tag="VFC15_14795"
     CDS             complement(7442..8407)
                     /locus_tag="VFC15_14795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM11472.1"
                     /translation="MQYRNFGKSGIKVSDIGYGAWGIGKSMWIGANDEESLRSLTAAR
                     DAGVNFFDTALAYGDGHSEQLIARAFGKSPEVIIASKVPPKNSVWDVKSGDPLQKTFP
                     KKYVLSCLDLTLKHLGRERIDVYQFHTWHDDWALDPEWQETVREMKSSGKVRLAGISI
                     QNHQPANVLKALDTGLVDSVQVIYNIFDQSPEDELFPYCEQHGIGVIVRVPFDEGALT
                     GKLTPETRFPDGDFRHHYFRGNRRQEIWEAVQAIVRETGITLDQLPELALRFCLSSPA
                     VSTVIPGMRNVHHVAANVAASDAGPLPPDVLQKLRAHRWVRNFWQ"
     gene            9200..9511
                     /locus_tag="VFC15_14800"
     CDS             9200..9511
                     /locus_tag="VFC15_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11473.1"
                     /translation="MEFLILVILILVPILRIVLRKRETMAVEQRQGTLCRSCANSHLV
                     KSFSGKELISCTFGGTLRPIKFEVRECTGFSNRLVTIAPLRVAGFVREESEVYAEIRI
                     A"
     gene            9712..10026
                     /locus_tag="VFC15_14805"
     CDS             9712..10026
                     /locus_tag="VFC15_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11474.1"
                     /translation="MSVYVKNGTPVGSQSQCAKCEHAHIMRGFRESEELTYCNSPTDL
                     VLVPFKVRECSNYSDKTRPTWDQMQDLAIEIRATPTLKPAGFFADQEPESEVAPEIPA
                     TR"
     gene            10296..12350
                     /locus_tag="VFC15_14810"
     CDS             10296..12350
                     /locus_tag="VFC15_14810"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZM11475.1"
                     /translation="MPLMNAFSKAVVRDFSIEELEQAAHLMRGYDLVALCAAGSGHSG
                     GTLSMMEIVAALYLKVANHDPNMPDWEDRDRIIWSGGHKAPALYLGLGFAGYYPLEDV
                     VTLRKLYSPFQGHPHRLKLPGVEVSSGSLGQGLSIAVGMALAGKMAGKKHTVFCIMGD
                     GEQQEGNIWEAVMEGSHFKLDNLVAIIDRNRLQIDGWVKDVMNVEPLKERYQAFGWEV
                     IEVDGHNIREVVEGLQKAKNIPVAGKPTLLLANTVKGKGVSFMENVAGWHGRAPTYDE
                     LVKSLDELGLKETIAYDALLKKAAEYQTEVEAKLEAKMPHFSRDYWWNAPNTPRVKME
                     PTRKGFGKSLANNGDDERIVCLGLDISGSITISDFYTGKPERKDRWISMGIAEQSATA
                     AAAGLAREGRLPVFGTYGTFAAARNLDQIRVSICYGNFNVMIAGAHGGVSVGPDGATH
                     QALEDLFAMCGLPNMVVAVPCDSIETRKATDYLLLQHVGPKYIRFAREATPIVTKEDT
                     PYVFGTANVIRLHRNAEQMIDAFDTVLAPDYRNEHEDLSIIACGPMVPEAMRAAYILK
                     QEFGYETRVLNIHTMKPIDEAAIVRAAKETGVVVTAEEHQIGALAWRVSSVITESPEL
                     FGTPVITGAIGVKDRFGDSGAPWELIKEFEVSAEHIAQKAAELIAIAKVKKEEEKLAL
                     AH"
     gene            complement(12669..13640)
                     /locus_tag="VFC15_14815"
     CDS             complement(12669..13640)
                     /locus_tag="VFC15_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11476.1"
                     /translation="MRSPFQEGFAVLREEPLLLGAELTSRWCFALAAWMLALAAAALF
                     LDSLKLSPGDRFLLRTLQPTLASTALSHVLHGTLLRYLWAKVVVAAGLTVLWCFAAAA
                     GRAASLRNLVALFGGDDRAEDAGWQFRPMFVLHCVRALWTWTAIGCLVGSIFLGTAML
                     QQQRAARAAFFYVFGTVLSLVFGVMLNWFFGLAPLFCIRDQATVRDSVSLTLDFCSRQ
                     GGRLFGLSLGFLALRIVWALSMLLLVLAPTSLAKHVAVGWILLLMFLLSLIYFAGSDA
                     LYLARLGAYAALAEIDAQPTPETAPEPDPVPEPQFVPINDISGSLRA"
     gene            complement(13700..14410)
                     /locus_tag="VFC15_14820"
     CDS             complement(13700..14410)
                     /locus_tag="VFC15_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZM11477.1"
                     /translation="MIRTFRPIPRKKIKLVLFDLDGTLIDSELDLANSVNAMLRHFGR
                     KELPLEVIDTYIGDGAPMLIRRALGDPAHAQVIQEALNYFLLYYREHKLDNTRPYDGI
                     LQALQQVEICDGIRRQMAVLTNKPVRASRDILAGLGLSQFFFQTYGGNSFETKKPDPL
                     GAQTLMSEAGAEADETVMVGDSEVDVLTARNANLWSIGVTYGFAPHTLERTHPDILVG
                     TPAELAQALSVVQAQTAG"
     gene            14575..>16102
                     /locus_tag="VFC15_14825"
     CDS             14575..>16102
                     /locus_tag="VFC15_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZM11478.1"
                     /translation="MATTKVEPKIAVAQPAPSEKYEGLTPQHLIEIYRLMYQSRKIDD
                     REIMLKRQQKIFFQISGAGHEALLVAAGILLRPGYDWFFPYYRDRALCLALGNTPYEQ
                     LLQAVGAADDPASGGRQMPSHWGNKKLNIVTGSSPTGTQFLQAVGCAEAGRYFNRYPG
                     ANEKRTGDYSEFKDVIFHGDEVVYVSSGDGTTSQGEFWESLSTASNLKLPMMFVIEDN
                     EYAISVPVEVNTPGGNISKVVQGFPNFHFAEVDGTDLLASYAAMKTAIDYVRAGKGPA
                     FVHGHVIRPYSHSLSDDEKLYRPESERKLEVERDPITRFRAFLLQEGILEEAALHEME
                     LDLTDEVQDAADQARGRREDHGRPHQLLPQGRDEARPAYRDLWRGRSRLQPRRVSARQ
                     AGERQGRRLQAHRRSAERVRLRPRIQLAAGRSQHCRPRHRNGHARHEARSRDPVLRLH
                     LARHAPATQRAANHPLALQQRILLPAGDSRRHRRIPHRRRHLSLAVRRKHLHPHAGHA
                     R"
BASE COUNT         3304 a         4691 c         4930 g         3177 t
ORIGIN      
        1 catcatgacc cgacgatgac gcaaacgaaa tatcgggcac atcgcgcgcg ttgtcgttgg
       61 gaacgcccag gccggtttgc cagggcggct tcaccccgtt ggaaaacgta ctcacgccgc
      121 caccggtggc agcaaaggcg gtaccagaag tggtgggagc tggggtgtcg ttccacgcga
      181 cctccggaat gtaagccaac gccgagggat tggtgtcatt cgttgatccc gcccagtatt
      241 gagtcgccgc agtgggcgcg ctgctgttgg tggagtccgc tgaaaaagtc gttcccccca
      301 tggcggttac ggtcggaagg cttgccggaa aatcgacggc aaggccttgg gtggcgggcg
      361 agttggcgtc gcaatcggcc gcgccgctat cgcccgaggg cgtgactatc gtctgtcctt
      421 gcgcattggc cagctgcccg gccgtggtca tagagctttg tgtggcgctg tccacctgcg
      481 gttcgcagag tccgtagctg atgctgagga tgggcgccag attttggtcc accgcatatt
      541 gcagggcgcc gaaagctccc tggctattgg cgttctcgat tacgtagatc agattggcgt
      601 tgggcgcgat cgctcccgac cactcgacat cgagattggc ttcatcaagt tccgcagtga
      661 ccagcccggg atcggtggtg ccggggacga ccacaatctg aaaattgctc gaagttagcg
      721 gcggcaagcc ggcgagaccg cgaaaggtca ccacatcgta ctgctgttgc tgtccattca
      781 ccacggcccc gggcgtgccg ttgcgaccgg ggttgctgtc agtggacagg tcagtttggc
      841 ccatgatcgc aatggtctgc ccggtgccat tgatgccgct cgcatagagt gcttgcaagt
      901 cataaatagt cgcgaaatcg ccgggcgcca caaaatggtt gccggtgaca gaagacgtga
      961 cccgcggctt cgccttggag ctgcgagctt gcggcgcgaa atcgtgcagt gcgccgatgc
     1021 cagtcactac tcccgcaaac gcagcaggaa tcgaaggctc ggacgaggcg gcgtagtgca
     1081 tcttgccgtt aaccaggtag ttgtgaattg acgtctggaa ggcggcgttc acttgtgctg
     1141 cgatgccgct gaaggcaatc caggtgcggc tgcgcgcggt ctggtccacg ctgaaaccct
     1201 gcgaggtcaa ccaggctgcg agggtattga tgtcctgcgc gctgacgccg aaccggtcgg
     1261 cgaattcctc gggcgtcagc catttgtgat agttcgacga agccgggtcc tgctgctcga
     1321 tcagcaactg ctcgagcgca gcttgctgtt ccgccgtcgg ctggaaaatg agcttcatgc
     1381 cctgcatggt ctgcgtgggc gacgtcgggc ctttatcgaa tgcagcgcga gccaaaggat
     1441 gcatcgtgcc ctgaagttgc accacgctgc ggttgtcaac cgggcgaacg atgcggttct
     1501 gctgcggcgc ctgccccgat gcgagtgagc taagaaaaat tatcagacca atcggtacaa
     1561 cggtgaatct ggacacaagg ttagattcca taaggtccct ggcgtggcag tgactggtcc
     1621 tgcggaccag caaacgcagc gcaattgcag gatatacata attcaccagc tagcgcgcga
     1681 aagttcctgt aagtcttgtt gcttaaaccg aattggtggc gagaagttgc gcgttgctca
     1741 gtcacggcgc gcgttacttg ctcgtgaaca gcagtggcgc gaagttaatc aggttccggc
     1801 gccacaccat ccaggtatgc gcgccaggag tttcgatgtc ggtgaacgcg atgtccttcg
     1861 atttgagcca ggccttgaat ttgcgattgt cgtcgttgag acggtcgctg gtgccgcacg
     1921 cgatccaaag cgtgcgcaat tgcgaagcct gctttgcgtc gagtgttggg aaggcaggct
     1981 cgtagtcgtc agacaatccg ccggcactga aggacccgac gtaggcgaac ttgtcgatat
     2041 tgttgagtcc ggtgagcagg ctctcagagc ctcccatgga gagcccggcg atggcgcggt
     2101 cctcccgctt ggtggaaacg ggataggcgc cttcaaccat cggcatcact tcggtgagca
     2161 gcgatttgcg gaagccgtcg aaattctgct ggcgcacgtc gggatgattc caggccgcct
     2221 gcttcagctt gatcatctcc ggcgtgccgt agccgagtgg catgaccaca atcattggct
     2281 tcaccttgct ctgcgcgatc aggttgtcga ggatgacgtt ggctttgcca accgccgtcc
     2341 acccgtcggc ggaatcgctg aagccgtgca gcaaatagaa gacgggatac ttcgtctttg
     2401 ccttgggatc gaagccgggc ggagtgtaca catagaaatc gcgctggtcc cccacgacct
     2461 tcgacttgaa gaagtgatga tgtatgacgc cgtgcggcac ttcggcgacg tcccacaact
     2521 ggggagtagg cccgctcacc tcgacgacat tttgcggctg aagcaggttc ggcttgaggc
     2581 tggagttgtt gggatcaaga aggtagacgc catcggcgat gaagatgtag ccgtagaggt
     2641 caggatcgag cggccccagc gtaatgctcc acaggccgga gtcgtcgcgc gtcatcggca
     2701 aaagtttggg cgcgccttcg aagttgagct gtacttcttt tgcgcccgga tcgaaaaagc
     2761 gaaatgtgac tctgccgtca gcctggactt cgggagactg gacgacgggc ggagtctgcg
     2821 cgtggatcgc gacagcgcca accgtgagca gggcaagaac aagaataaga gacaggcgac
     2881 gtgacatacg agtcctcaag tgtgagtgcg acgcagcagc gttagtgtaa acgcgatggt
     2941 ggcgaagtgt cgcagcgccg ctgaagtatg cggctctcgc ctgcccaacc gacgtgacta
     3001 cttcggttta tccgcgtcag tctttggtac ggtcttggtg tccttagacg cgctggagtc
     3061 tttcggcgga ggcttgagtc cgcgggtgga catgcgctcg tcggttttga ccttatcgcc
     3121 gtccgtcgag ctcttcgtcg aggccgcttt cttcggattc gccgccggct tcttcggtgg
     3181 gttgttcgtg gtctggtctt gctgaggagg acccggcagg ggcgacgcga cgttcggact
     3241 caccagcaaa atcagcgtgg cgatcgacga cagcgtcgtg aggagcaatt tcttcatgag
     3301 cgacctcctg ggaatggggg cggacgtagc cttcaatcgc aggatgcagc aagagtagca
     3361 ccagaggaaa catgaaatcg agacaacgat gaaaataggt agcggcaaag taggctagtg
     3421 cggcgggcac caggagcagc agcatcacca gcaagcgtgt cccggttccg cgccgcaggc
     3481 ttacgatcca caacatcagg atcgcgagtc ccacgtcaat cagcgccgct gcccagcgat
     3541 ttaccttgaa cagtatgcga ccggaaagca ggccttcgac gtagttggcc tgatagtaca
     3601 ttccgcgaat cggaccgacc gggctgtcgt ggtcgtcgag atagtcgtcc tcgtcatgct
     3661 cacccttgta agcgtggcgg ttacctccga tgagcacgat gtggccgctt atcaattcga
     3721 gagccagttg cattcgggtg cgattctgtt ggtcgcaaat ttgcatgttc cagttctgct
     3781 ttttctcctg cttcttctca aaaccagggc tgcaaaaaac ctccacggcg gaaagagagc
     3841 cagtgtggtc gcggtccact tggagtctgg gatccacgaa cgcgctgacc ggcatgaaag
     3901 acgtgtacac aaactcgtgc ctggtcaggc gatcgacgag ggctttatcc tccaatgcag
     3961 tcttgttccc tctgtgctca aaactatcgg tgatctgcag agcgaaggaa tagaaatcga
     4021 aactgtttcc ctggtcgtcg tggccgacgg cgactaacgg gactttacga aaatcgtctc
     4081 ccgggtgatc gaatccgaat cgcgcaatcc agtacgggcg tggcggactc tcgtcctcag
     4141 ggaattcgta cgaaatgcct ttgccttcat agaccacttg attgtcgaag acttggggaa
     4201 ccgggacctg gtcgcggcgc agaccgaacg tgagcacgac cggtattttc acagcctgtg
     4261 cacggcggat ggcgtccaaa agcgcttgat cgtcggcgtt aagcgactca ggagcgtcgc
     4321 gctgcttgtc gaagcgggcc tcatccagat tgatatcgag agcgatgaca gccgcctgag
     4381 cttgcgtggc cgcatccacg agttgcgcga ggaacctgcg atccgtgatc tcgtcacgcg
     4441 tctcgttgcc gaagaagtcg taaaacgttg tgtcgtcgat ctcaataggc gtgaccgact
     4501 ccgcgttcgg cttacccagc ccgagatcgg tgatccattg ataggtcctg agttgccagg
     4561 tgatgagccg ggggaagtcg tccttgaagt cttcgtactt gaagctgagg acgcccacta
     4621 tgacgatcag caacaaagcg ggaatgtcga gccagggaaa tctccgcgat agggcgcgcc
     4681 aggaaatcat cacgggccac ccgaagcagc ggctgcagag gtggtgggaa caccgagcac
     4741 gatcaggtcg ccctggtcgc tctgcgccat caccgggatt tgttgctgct tggcgcgcgt
     4801 agccacctgt tcgcgaacaa aaagatacac cttgccgatg gggtccatgc ccttgctttg
     4861 cttgagcgcc tccagcaggt aatacgtgaa atagccgtgc tgcagcgacg cgctctccag
     4921 cgactcctgg ttttcctggc tggcggcgag gatcacgcgg ccggcgcctt cgctcatgcg
     4981 actcagggtt gcgccggaaa cggatttcag ccccattgcc gatgcgaccg tcttagtccc
     5041 accggagccg gcagccgcgc cgctgaaaca agtgtccaga aacaccgcga cccgctgagc
     5101 atgcacgcga ctggcgacgg tgttggtcac gtcaaccatg ggcagcgcgg tggcgaacag
     5161 ggcgtcgtgg tcgatgtcct tgtcggtgtc gccggtagtg tcgccggcag cgtggcgctt
     5221 gggcgaaatt tcagtgtcgg cggtgatgat gtaatttacg cccgcggtat cgaggtcgcg
     5281 cgaggagccg tgggtcgcga tatagatgac aaccaaatcg tcaggctgcg cggtccgcgc
     5341 cagccagttc aacttctcgc ggatgttctt ggtagtcgcc tggtagtcgg tgagcacggc
     5401 gacgttctgt ggcttgaagc ggccgacacc cgcgttcttc agcgtgtcgg caaagccagt
     5461 ggcgtcggcg gtggtgtagc gcagcgtggg gatggccttg tcctggaact ttccgatgcc
     5521 gacgatcagc gcccactttt cccgcaccgg tcccgcgggc ttgaccgccg gattggtgga
     5581 aagcacgaaa ggatcgagct gctgctgcaa cgcttctgag ttgttctggc gcgccttctc
     5641 cagcgagtag tcggccagtt tcgtattccc cagcttgcgc tcgagcagcg agcgatagta
     5701 ccaggcatcg ccaagactga tgcactccga ggtagcatgc ttgagcagtt cgacggcatc
     5761 gcgaagctgc gatggggtgc tactcggttg cacgcgctcc agagcctgca ccatcaggtc
     5821 ggtgccggca ccgcagcgct cctgcgcggc agtgaagctg gcgcagagaa gaagcgcagc
     5881 gagcgcgaag cgcgaaatgg agcgaagcag catggttagt gcgacgacaa ctgacgcgcg
     5941 cgcgtcttca tcgcggttag cacttttccc ttcacaccgt tctgcgccag cttcacttgg
     6001 ccgtcgacgc tcagatcgaa gttcactgcg ccagccgagt ggatcgtgtc aataatgttg
     6061 tcctcatcca cacctgcctt caccatttga atgacctgat cgttgttgag cgaggggccc
     6121 gtcgccttct tggctgcggg ggcgggcgac ttggccgcag tcgtgccgct gctggaactc
     6181 gtcgacttgg ccgaggcgct cttagtagta gttgaggttg agccgccgga actgcgtccg
     6241 gatttggccg acttcgccga gtcatcggac ccggattttc cgccgctgtc ggctacggcc
     6301 ggtgcgctgg gctgttcgcg caacttctta tagtgagcga ttgcggtgtc gatgcggttc
     6361 tgcggctgca ggaagtactt ctcctccggc ttgtcgtcaa tcgccttgcc gtaattgatg
     6421 gcggcttcct gcagatactt ctgcgccgcc ttggcatcct cagactggta ggccagcgcc
     6481 tcgtaggcca ccccgatgtt gtagagacgg tacgcgtcgt cctccttgct ggggaagggc
     6541 gtcatggttt cgagggcttc cagattacgc gtccacaggt tggcttcggc gagcttgtcg
     6601 gcgtcgtcga gcttgccgcg tgccagcagg acttccacag gctcctcggt gttcaccagc
     6661 cgagaggcga tttgcgagac cacgttgctt agcaacacgt cgcgcaattc ggcggcggtg
     6721 ggcggtttgt cgccctcgag tttaccgtgc ttgagcttgt cccaggtgtg cgtcagcgtg
     6781 cccgtgacac cgcccgaggt ggaaccgtac tggttgaact cgtcgtcata cttggcggtg
     6841 acattgtccg agtcgagcgt gcgtccactg tgcgcgtctt tcgcttggta cgcgactttc
     6901 aggattccgg agtagcgggt cacagtttcc tgctgcggct ttttggaccc cggaacgtag
     6961 gtggagtgcg acgaagtctg tgcctggggc ggagcgtatt cggtaatcgt gcagatcacc
     7021 agcgaatcgg gatgcttgtc ttcggagcgc agccggcggt cgtccttcag cagttccgtc
     7081 tccagcatgt tgcgcaagtc ggtcgcaacg tcgccctgta tgttgtgggc ggtgacctgg
     7141 acatcgatgg cggtgccatt gagatgcgcg gttggcggca acttgcgcag cagcgtgatc
     7201 ttcttcttgg acgacccgaa gccctgcgca aaggccaggc cagacccata cgtgaccagt
     7261 aacaaagcgg ctgcgggcag ccgcaccggc agcgatcgca tcacttgtgg gaaacgaagc
     7321 atggcataca tccaatcggg acctgtcgcc ccatggcgca tggaaggtga ccgaaaagta
     7381 cgagcactat agcaagattg ggggcggcaa gtcatgcgga atgcaaaacc ccggtcccga
     7441 gtcattgcca gaagtttcgc acccagcgat gtgcccgcag tttttgcagt acgtcgggtg
     7501 gcagcggtcc ggcgtcggag gcggcgacgt tggccgccac gtgatgcacg ttgcgcatcc
     7561 ctggaatcac ggtcgagacc gccggagagg acaggcagaa gcgcagcgcg agttccggaa
     7621 gttgatcgag cgtgatgcca gtctcgcgca cgatggcctg caccgcctcc catatttcct
     7681 gcctgcgatt gccgcgaaag taatggtggc ggaagtcgcc gtcgggaaaa cgcgtctcgg
     7741 gcgtgagctt tccggtgagc gcgccttcat cgaagggaac gcgcacgatc acgccgatgc
     7801 cgtgctgctc gcagtaagga aacagctcgt cctccggcga ctgatcgaag atgttgtaga
     7861 tgacctgcac actatcgacg agtccggtgt cgagcgcctt gagcacgttg gcgggctgat
     7921 ggttctgtat ggagatgccc gccagccgca ccttgcctga ggatttcatc tcgcgcaccg
     7981 tctcctgcca ttcgggatcg agggcccaat cgtcatgcca ggtgtggaac tgatagacgt
     8041 cgatgcgctc cctgccgagg tgcttcaaag taaggtcgag gcaggagagc acatatttct
     8101 tggggaaggt tttctgcagc ggatcgcccg acttgacgtc ccagacgctg tttttgggcg
     8161 gcaccttgct tgcgatgatg acttccggag acttgccgaa ggcgcgggca atgagctgct
     8221 cgctgtgacc gtcgccgtag gccagcgcag tgtcgaagaa gttgaccccg gcgtcgcgcg
     8281 cagccgtaag cgagcgcaag gactcctcgt cattggcgcc gatccacatg gacttgccga
     8341 ttccccaggc gccatagccg atgtcgctga ccttgatgcc cgatttaccg aagttgcggt
     8401 attgcatgag tacctcgcag tgtctgaaat gatagcgcgt cggactgcgc agcggcacgg
     8461 caggcacccg acgggagcga accgcaccac caaagtaggg aagtataacg acactaacca
     8521 ctggctctcc gccaccatgg ttcgatgcgg ctgattttag ttaagaaata agaaatatga
     8581 tccaaattga attttgcgct tgcatttttt atggagatat agcaaggtat aactcgcgta
     8641 ggagaacgcc cgatgtcact tacaacgcac aactcgatgc gtagggcacc gcaaccaaga
     8701 ctggtatgca gtgaccgccg ggctatcttt ggaaattatc gctagctcct ggcgattgaa
     8761 tcctacgtaa ccgcaaacca tttaaagctt gctgggtagt aaagaccacg gtcgtttcga
     8821 ctcacggtca atagcaaacc gtaggcgctc gttctcccag catcaagttt ggccagcgcg
     8881 aagtgtacag atacttccag gatgccccat ctgggcgggg cgtggaatcc agcaaatcat
     8941 ggacgctggt tccctggcca aggactctcg caggattaac gcctgcgaga gcgggctagc
     9001 gccgttggtg aaggaaacag ttagtactct cgcccagaag agcgcagggg ccgaaatccc
     9061 tgccggaacc agcgattgca cggatcgtag catagcttcg tacttcaaga cccatgccgg
     9121 aagcggtccg gtcttaactg tttccgattg ttccccggcg cgatttggaa aggaagtagc
     9181 agaagtaacg aggtgagtta tggaattcct gatactcgtg attttgatcc tggtcccgat
     9241 attgcgaatt gtcctccgca agcgcgagac catggccgtc gagcagcgtc aggggacgct
     9301 ctgccgttcg tgtgcgaatt cgcacctggt caagagcttc agcgggaagg aactcatctc
     9361 gtgcaccttc ggaggcacct tgcgtcctat caaattcgag gtacgcgagt gcaccggatt
     9421 cagcaaccgc ttggtgacca tcgctccact gcgagttgct ggattcgtcc gggaagagag
     9481 tgaggtatac gcagaaatcc ggatcgccta gtcgttaacc agagtattaa tttttggccg
     9541 gtggtgtaga ccggactact caaactaata gtggggtcgc aggttcgagt cctgcggcgg
     9601 gcccaaccat gcccgtaatc aggtaataga aatcgcaatg tgagtcgatt gcctgttccc
     9661 ccggccgaac catgaccttc ggcaggagcg ccgattagaa agcagttcgc tgtgtctgtg
     9721 tacgtcaaaa acgggacgcc ggtcggatcg cagtcgcaat gcgcaaaatg cgagcacgcg
     9781 cacatcatgc gcggttttcg cgagagcgaa gagctcacgt attgcaactc tccgaccgac
     9841 ctggtcctgg tgccgttcaa ggtccgcgag tgctccaact actcggacaa gacgcggccg
     9901 acctgggacc agatgcagga cctggctatc gaaatccgcg ccacgccgac tctcaagccg
     9961 gcgggttttt tcgcggacca ggagcctgag agcgaagtgg caccggaaat acccgccacc
    10021 cgctaaccat caacctccgg ggaggaaggt gcagccttcc tccccggaat cgcaagcccc
    10081 cgctaacctc agcgattttc ctttgtggca aacgtcacat ctgctcgtga tagcgcgcac
    10141 tgcgagtcgg ttcggtccgt cctagccttg agcttcgggc cccaagtgca gcgggtaatt
    10201 ccaatgctat gtgttgaacc acgtcgggcg gtgccgtatt gtgcatcggc caatccggtg
    10261 tgggaacctc gccctgcccg caagaaggaa acgacatgcc tctgatgaat gctttctcca
    10321 aagccgttgt ccgcgacttt tccatcgaag aactcgagca agccgcccac ctgatgcgcg
    10381 gctatgatct ggtcgcactg tgcgccgccg gctcagggca ctctggcgga acgctctcca
    10441 tgatggaaat cgtcgccgcc ctctacttga aggtcgccaa tcacgatccc aacatgcctg
    10501 actgggaaga ccgcgatcgc atcatctggt cgggcggaca caaggctccc gcgctttatc
    10561 tcgggctcgg attcgccggc tactacccac ttgaagatgt agtcacgctg cgcaaactct
    10621 actcgccctt ccaggggcac ccgcatcggc tgaagctgcc gggcgtcgaa gtgtcgagcg
    10681 gatcgctggg acaaggcctg agcatcgccg tcggcatggc gctggcgggc aagatggccg
    10741 gcaaaaagca caccgtgttc tgcatcatgg gcgacggcga gcagcaggaa ggcaacatct
    10801 gggaagccgt gatggaaggt tcgcacttca agctcgacaa cctggtagcc atcattgatc
    10861 gcaaccgtct gcagatcgat ggatgggtga aggacgtgat gaacgtcgag ccgctgaagg
    10921 agcgttatca ggccttcggc tgggaggtca tcgaggttga cggccataac atccgcgaag
    10981 tcgtcgaggg attgcagaag gcgaagaaca ttcctgtggc cggaaagccg acgttgctgc
    11041 tggccaacac ggtcaagggc aagggcgtca gcttcatgga gaacgttgcc ggatggcacg
    11101 gccgcgctcc cacctacgac gagttggtga agtcgcttga cgaactcggc ctgaaagaga
    11161 ccattgccta cgacgcgctc ctgaaaaagg cggctgagta tcagacggaa gtcgaggcca
    11221 agctggaagc caagatgccg cacttctccc gcgactactg gtggaacgcg ccgaacactc
    11281 ccagagtaaa gatggagccg acccgcaaag gcttcggaaa gtcgttggcg aacaatggcg
    11341 acgacgaacg catcgtgtgc ctgggtctcg acatttcggg atcgatcacc atcagcgatt
    11401 tctataccgg taagcctgaa cgcaaggacc gctggatcag catgggtatc gccgagcagt
    11461 cggcgaccgc ggctgccgcg ggactggcgc gtgaaggcag gctgccggtg ttcggaacgt
    11521 acggaacatt tgcagcagcc cgcaacctcg accaaattcg cgtgtccatt tgctacggca
    11581 atttcaacgt gatgatcgcg ggcgcgcacg gcggcgtttc cgtcggacca gatggcgcga
    11641 cccaccaggc gttggaagat ttgttcgcca tgtgcggcct gcccaacatg gtggttgcgg
    11701 ttccgtgcga ctccatcgag acccgcaagg cgacagacta cctgctgctg cagcatgtcg
    11761 gtccaaagta cattcgcttc gcgcgcgagg cgactcccat cgtcacgaaa gaagatacgc
    11821 cgtatgtgtt tgggacggcc aacgtcatcc ggctgcaccg caatgccgag cagatgatcg
    11881 atgccttcga taccgtgctt gcgcccgact atcgcaatga gcacgaagac ctcagcataa
    11941 tcgcctgcgg ccccatggtt ccggaggcga tgcgcgccgc ctacattctc aagcaggaat
    12001 tcggctacga aacgcgagtg ctcaacattc acaccatgaa gccgattgac gaggcggcga
    12061 tcgtgcgtgc ggcaaaagag accggcgtcg tggtgaccgc cgaagagcac cagatcggcg
    12121 cgctggcgtg gcgcgtgagc agcgtcatca ccgaaagccc cgagttgttc ggaacgccag
    12181 tgatcaccgg cgccatcggc gtgaaagatc gcttcggcga ttcgggcgcg ccgtgggaac
    12241 tgattaagga gttcgaggtc agcgccgagc acatcgctca gaaagcggcc gagttgatcg
    12301 ccatcgccaa agtgaagaag gaagaagaga agctcgcgtt ggcgcactag atcaatggtt
    12361 tttgaaaggg catgccttca cagcctgctg aaaattcgac ttgtcttaaa agggcatggc
    12421 ttcagccgtg ccgtacaagc cttttatttt tttgtcatgc ctcgcgggca tcagcccgcg
    12481 gggaatctgt ttttctgacc tcatctatag cctgttcggg atcgcatcag ccggaatgca
    12541 aaaactatgg aattcgcaag cccatagccg cgcagcggcg gcacttgttt tagccccggg
    12601 cgtaagcccg gggtaagcgt ccctttaaat aacgagagcc gcgtagcggc gacaccgctc
    12661 cgtgccaatt acgcgcgtag gctgccggag atgtcgttaa tcggaacgaa ctgtggctcg
    12721 ggcacgggat ccggttccgg agccgtttct ggcgtcggct gcgcatcaat ttccgccagc
    12781 gccgcataag ctccgagtcg cgccaaatac agcgcatcgg atcccgcaaa ataaatcagg
    12841 gacagcagga acatcagcag cagtatccat cccaccgcga cgtgtttggc caaactggtc
    12901 ggagcgagca ctaacagtag catcgaaagc gcccagacga ttcgcagcgc caggaaacca
    12961 agcgacaacc caaacagacg tccgccctgt cgcgagcaaa agtcgagtgt caacgacacc
    13021 gagtcgcgga cggtcgcctg atcgcggata cagaacaacg gcgccagtcc gaagaaccag
    13081 ttcagcatca caccgaagac gagcgacagc acggtgccga agacgtagaa aaatgctgcc
    13141 ctcgctgccc gctgctgttg cagcatcgcg gtaccgagaa agatcgagcc aaccaagcaa
    13201 ccgatggcgg tccacgtcca caaggcgcgg acgcagtgca acacgaacat cgggcggaac
    13261 tgccaacccg catcttctgc gcggtcgtcg ccgccgaaca gcgcgacaag gttgcgcagc
    13321 gaagcggcgc gtccagcggc tgccgcgaag caccacagca ccgtcaatcc agcggcaacg
    13381 acgaccttgg cccagagata acgcagcagc gttccatgca agacgtgaga cagtgccgtc
    13441 gaggccagcg tgggctggag cgtcctcagc aaaaaccgat cgcccggtga aagcttcagg
    13501 ctatccagaa agagcgcagc ggcagcgagc gccagcatcc atgccgcgag ggcgaagcac
    13561 caccgcgacg tcagctcggc gcccagcagc agcggctctt cgcgcaagac ggcaaagcct
    13621 tcctggaacg gcgaccgcaa tctgccaggc tgcgacatgg catcagaata gcagcgaagc
    13681 gcgccgatgc cgagatttcc tacccggcgg tttgagcctg aaccacgctc agcgcctgcg
    13741 ccagttcggc gggtgtacct accagaatgt cgggatgcgt tcgctccagc gtatgtggcg
    13801 cgaagccgta ggtcactccg atgctccaca aattggcatt gcgggcggtg aggacatcca
    13861 cttcggagtc gccgaccatt accgtctcgt cggcttccgc gccggcctca ctcatcaacg
    13921 tctgggctcc cagcggatcg ggcttcttgg tttcgaagct gttgccgcca tacgtctgga
    13981 agaagaactg agacagcccg agtcccgcca gaatgtcgcg cgacgcacgc accggcttgt
    14041 tggtcagcac cgccatctgc cgccgaatcc cgtcgcagat ttcgacctgc tgcagcgcct
    14101 gcagaatgcc gtcataaggg cgagtgttgt cgagcttgtg ctcgcgataa tagagcagga
    14161 agtaattcag cgcttcctgg atgacttggg cgtgtgcggg gtcgcccagc gcgcgccgta
    14221 tgagcatggg agcgccatcg ccgatgtaag tatcgatcac ttccagcggc aactccttgc
    14281 ggccgaagtg gcgcaacatg gcgttcaccg agttggcaag atcgagttct gaatcgatca
    14341 gggtgccatc gagatcaaac agcacgagct taattttttt gcgcggaatc ggccgaaaag
    14401 tgcgtatcat tccttttcca gtatagccac ctgaccgcga acggcagaca ggctgcttga
    14461 gccgggatca accgcgggcc gagccccaga ttcgggccaa tcgtccccgg ggatttacaa
    14521 taggtcaagt tctgagccgt tagcgggtcc ctcatttcaa gcccgaggtc atggatggca
    14581 accaccaagg tcgagccgaa gattgccgtt gcgcagcctg cgccctccga aaaatatgaa
    14641 gggctaacgc ctcagcacct catcgagatc taccggctga tgtaccagtc gcgcaagatc
    14701 gacgatcgcg aaatcatgct caagcgccag cagaagatct tcttccagat ctcaggcgcc
    14761 ggacatgaag ctctgctggt ggccgcggga atcctgctgc gtcccggtta cgactggttc
    14821 tttccttatt atcgcgaccg cgcgctgtgc ctcgccctgg gcaacacgcc ctacgaacaa
    14881 cttttgcagg ccgttggggc cgccgatgac cccgcttccg gcggacgcca gatgccttcg
    14941 cactggggta acaagaagct caacatcgtc accggatcgt cacccacggg aacgcaattt
    15001 ctgcaggcgg tgggctgtgc cgaagcagga cgctatttca accgctatcc cggcgccaac
    15061 gaaaagcgca ccggcgacta cagcgaattc aaggatgtca tcttccacgg cgacgaagtg
    15121 gtttacgttt cttccggaga cggcacgacc agccagggcg agttctggga gtcgctcagc
    15181 accgcctcga acctcaagct gccgatgatg ttcgtgatcg aagacaacga gtacgccatc
    15241 tccgttccgg tggaagtcaa cacgccgggc ggcaacatca gcaaggtggt acagggtttc
    15301 ccgaacttcc atttcgccga ggtcgacggc accgacctgc tcgccagcta cgccgcgatg
    15361 aagaccgcca tcgattacgt tcgcgccggc aagggtccgg ccttcgtcca cggacacgtc
    15421 attcgtccct actcgcattc gctctccgac gacgagaagc tctaccgtcc ggaatccgag
    15481 cgcaagctcg aagtcgagcg tgatcctatc acccgcttcc gcgcattcct gctgcaagaa
    15541 ggcatcctcg aagaagccgc gctgcacgag atggaactcg acctcaccga cgaagtgcag
    15601 gacgccgccg accaagcccg cgggcggcga gaagaccatg gccgacctca tcaactcctg
    15661 cctcaaggac gagatgaagc gcgacccgcg tatcgtgatc tttggcgagg acgtagccga
    15721 ctgcagccgc gaagagtatc tgcgcgacaa gcaggtgaaa ggcaagggcg gcgtcttcaa
    15781 gctcaccggc ggtctgcaga gcgcgttcgg ctccgaccgc gtattcaact cgccgctggc
    15841 cgaagccaac attgtcggcc gcgccaccgg aatggccacg cgcggcatga agcccgtagt
    15901 cgagatccag ttcttcgact acatctggcc cgccatgcac cagctacgca acgagctgcc
    15961 aaccatccgc tggcgctcca acaacgcatt ctcctgcccg ctggtgattc gcgtcgccat
    16021 cggcggatac ctcaccggcg gcgccatcta tcactcgcag tgcggcgaaa gcatcttcac
    16081 ccacacgccg ggcatgcgcg tg
//
LOCUS       DASRKK010000614         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_641485, whole genome shotgun sequence.
ACCESSION   DASRKK010000614 DASRKK010000000
VERSION     DASRKK010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641485"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..2202)
                     /locus_tag="VFC15_14830"
     CDS             complement(490..2202)
                     /locus_tag="VFC15_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11479.1"
                     /translation="MTRNTARIIGSSMLAMAVLALCALPLVAQEITVNHATASGVTAP
                     LRELAKLPQPMQYGFHEAAPYRRIAKRDFGTSVDPVEQSSAAPSTNYSIIANFLGVGN
                     GFPGYTVPDAPPDTNMAVGDTQVLQWVNVSYTICSKTSPYTCGPAIIGNTLWHNGIPG
                     TLCANSNAGDILAQFDRKADRWFLSQNVFSSPYAVCVAISDTNDATGTWHVWQFPVPG
                     AGFPDYPKFATWPTSGVNNGYYQAWNNFGPGGSGFQGPKICAYDRAAILAHAASPTQI
                     CSQLSSSEDSLLPGDLDSILGVPNTPAGTPQDEFYIGSVGDVDNSHLSLYQFHADFTT
                     PANSFVLGSPNTILQPVPTYTGSCSGGFGGDCVPSLGGSPMDSLGDRLMYRFAYWNDG
                     PAPSAAPCAICVVPHFQHWYVNGDVESSGGEIGVRWYEFRAPPVPGIGTLTSTTLTTF
                     QAGTYAPDSTYRWMGSLTQDKAGDILLGYSQSSATTHPAIYVAGRINNDPVGLNNLES
                     ELLVVQGNGSQPDTSDRWGDYSSMRIDPVDNCTFWYTTEYYQVTQSFDWSTRINSAKF
                     SNCN"
     gene            3011..>4560
                     /locus_tag="VFC15_14835"
     CDS             3011..>4560
                     /locus_tag="VFC15_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11480.1"
                     /translation="MFAQKTVVQDGGGGRKLEFDYNAAGQVTQQRTLAADGKLEQKVD
                     YEHRPGYYVADQTTTFYWPDGKVRRTTKNTYEASANFTGEFVQDFDEAGKQVGGHRLT
                     HDPMTNVYTCADWNTAAQDYKLVECPAGEESAGGSEEVKKFTYDEVMLHLEAARKAVG
                     EGQKIQPKVQVEATATTANQEFGLVVPAQVYPGERVSGSIVANPAEYEPMAEVTVTQV
                     AVPRESAGEASTLAGWSVEVPGESPQRADGPIAFTVPRGSSELNITLQQTGNSAHSVS
                     KPIRFPQSAAKKQKAPNSFEAAALCLKGQLCTVRGPFSGDSSKTFAAFEDRSAMVVAE
                     TPDTAYIRIPNLTEPGSRPLFIAEGSKVVALPVAVGEFTLKNGDREVQPGQTLVTFPT
                     LEGPSDISDAAWLPGNFPVTNLEEARKLIPGFQLPRDDQKPRQEREAQQKHESKEKRE
                     ADEKKGGSILLVVKNLTPEQVSMRGTKDQMIVFHLKDASFSRGEFKYDLIVEAIKSGH
                     FDIRGYVIP"
BASE COUNT         1040 a         1220 c         1380 g          920 t
ORIGIN      
        1 aataggtaaa gcggccagat gtcgagtgtc ccgccagcaa gatccgcacg gcacggcgcc
       61 gtggtcacga tctcgtcgct gttcaccggg gacatgcgtt gcggagcggt tggcatgatc
      121 actttccgat cgcggacgga accctgtacc aaggcagttc aggaattact ctatcccttc
      181 gagccctggt agagccgcgc tttatttgga ttattcatct gcttcggcaa ctgagccaac
      241 gagctttgaa agggcacggc ttgaagccgt gccccttcaa agcgtgaagt cctcacgaat
      301 aaaccgcata agcgccccaa ggcaaggctc tcggactcgt gcggcaggcc gggttgacaa
      361 tcctctgtca agagcgcggc caatcggaga aagaaacagg gcatcccgtt gccggaatgc
      421 cctgtggttg aggttagtta ctggttgcat cctcgccggg ccgtaaggct cagcggaggt
      481 ttcttccagt tagttgcagt tcgagaactt cgctgaattg atgcgggtgc tccagtcgaa
      541 gctctgcgta acctggtagt actcggttgt gtaccagaag gtgcagttgt caaccggatc
      601 aatccgcatg gagctgtagt cgccccagcg gtctgaggta tccggctgtg aaccgttgcc
      661 ctgaaccacc aacagctctg attccagatt gttaaggcca accggatcat tgttgatccg
      721 tccagccacg taaattgctg ggtgcgtagt tgcgctggat tggctgtaac ctaacagaat
      781 gtcgcctgcc ttatcctggg taagagaacc catccaacgg taagtgctgt cgggagcgta
      841 cgtgcccgcc tggaaagtgg tgagagtggt agaggtcagg gtgccgatac cgggtaccgg
      901 cggggcccgg aactcatacc accggacgcc tatctcgcca ccggaggatt ccacgtcgcc
      961 gttcacatac cagtgctgga agtgagggac cacgcaaatg gcgcagggtg cggcgctggg
     1021 cgccggacca tcattccaat aggcgaagcg atacatcaag cgatcaccca gggaatccat
     1081 gggagagccg ccaaggctgg gaacgcaatc gccaccaaat ccaccactgc aggaaccggt
     1141 atacgtgggt accggctgga ggattgtgtt gggactgccc agaacgaacg aattagcggg
     1201 agtcgtaaaa tccgcgtgga actgatacag agacaagtga gagttgtcca catcgcctac
     1261 gctgccaata tagaactcgt cttggggagt tccagccggg gtgttaggca caccgagaat
     1321 ggaatccaag tcgccaggca acaagctgtc ttcgctggac gacaactggg agcaaatctg
     1381 tgtcggactg gcagcgtgcg ccaggatggc ggcgcggtca taggcgcaga ttttgggacc
     1441 ctggaagccg ctgccgccgg ggccgaagtt gttccaagcc tggtagtagc cgttgttgac
     1501 gccgctggtt ggccaagtag cgaacttcgg gtagtcaggg aagccggcgc caggtaccgg
     1561 gaactgccaa acatgccagg tgcccgtcgc atcattggtg tcggagatag cgacgcagac
     1621 cgcgtacgga gaagaaaaga cattctgcga caggaaccag cgatcggctt tccggtcaaa
     1681 ctgggcgagg atgtcgccag cgttgctgtt ggcgcacagg gttccaggaa tgccgttgtg
     1741 ccacagcgtg ttgccaataa tggcgggtcc gcacgtgtag ggtgacgtct tggaacaaat
     1801 ggtgtaggaa acgtttaccc attgcagcac ctgggtgtcg cccaccgcca tgttggtatc
     1861 gggaggagcg tcaggaacgg tgtatcccgg aaagccgttg ccgactccaa ggaagttggc
     1921 aatgatcgag taattggttg agggagcagc gctgctctgt tccactggat ccacggaggt
     1981 tccaaagtcg cgttttgcaa tgcggcgata gggagctgct tcgtggaagc cgtattgcat
     2041 cggctgtggt agcttcgcca gttcgcgaag cggcgcggta acgccagacg ccgtcgcgtg
     2101 gttcacggtg atttcctgcg caacgagcgg cagggcacag agtgcgagta cggccatggc
     2161 gagcatggac gacccgataa ttctcgcggt atttcttgtc atatcgatcc cattctcctt
     2221 aactgatcta cgcggcgcat tgggtatcgc ttgcgcgcag tagtcttcgc aactcggcag
     2281 gtgtgtggtt ggaacacggt tcttcgtgtc ttcgtagttg ggcaattgtg tagttgggca
     2341 attgtgattc gttacctgtt tgaagtgttt ctaggcaggt ggaaagatta aaacacaagg
     2401 actgccgcaa tgcaactatt attcacgtag ttactaggcc gccactacaa gccgagcccg
     2461 ccaccttcaa gaccgtttgc cactcgctgt agtgcagcta actcctgtgt acatcgtggc
     2521 ttgaggcgag gagagagtat cggagtgccg ttttgggcca gctcataccg caaaaaatgt
     2581 cacagaaatt ttacaaatgg cacagatcga atgcggctgc ctcgcatttc ctttttgggg
     2641 tcagcacctt gtcgtggcca gcaagggccc gtgaaaacta acgtcccgga cgccgccctt
     2701 ctgcgaaaga acgaatataa gggttgttct tttctttctc gcttggcgcc gctcctggtg
     2761 gccagatcgt gggtggccgt cgtgtggagg gtctccgtgc taaactagaa atttgaccgc
     2821 tactgggcag cctcagtgtg tgcgcggcaa tcgtagggct tccattccag ctccctgagt
     2881 tttcaaaact aatcgacggt aagattctcg ccaaggggaa agacacgtgc gacaaaatca
     2941 tgaccattac ccaccgcgta tttggctcag ctttgctttc ttcacggtcc tgatgtggag
     3001 tgcaacacca ttgttcgcac agaagaccgt ggtgcaggac ggtggaggcg gcagaaagct
     3061 ggagttcgac tacaacgctg ccggccaggt gacgcagcaa cgcactctcg ccgcggatgg
     3121 caaactggag cagaaagttg attacgagca tcggcccgga tactacgtgg ctgaccaaac
     3181 caccactttc tattggccgg acggcaaggt ccgccggacc accaagaaca cctacgaggc
     3241 cagcgcaaat ttcaccggcg agttcgtcca ggacttcgac gaggccggca aacaagtcgg
     3301 cggacatcga ttgacccacg atcccatgac caacgtctat acctgcgcgg attggaacac
     3361 ggctgcccaa gactacaagt tggtcgagtg tccagcaggt gaggaatcag caggtggatc
     3421 tgaagaggtc aagaagttca cttatgacga ggttatgctg catctggagg ccgcccgaaa
     3481 ggcggtcggc gaaggacaaa agatccaacc aaaggtacag gtagaggcga ctgccaccac
     3541 ggcgaaccag gagttcgggt tggtcgtacc ggcgcaggtc tatccgggcg agcgagtatc
     3601 gggcagcata gtcgcaaacc ccgccgaata tgaacctatg gcagaagtga cggtcaccca
     3661 agtcgcggtg ccgcgtgaat cggcgggcga agcgtcaaca ctcgcaggct ggagtgtcga
     3721 agtgcccggc gaaagcccgc agcgcgccga cgggccgatc gccttcactg tgccgcgtgg
     3781 cagttcggaa cttaacatca cgctccagca aacggggaat tccgctcatt cggtttccaa
     3841 gccaataagg tttccccaaa gtgccgccaa gaagcagaaa gcgcctaatt cgtttgaagc
     3901 agcggcgctc tgcctgaagg gccaactgtg caccgtccgt gggccattca gcggagacag
     3961 cagcaaaacc ttcgccgctt tcgaagatcg ctcagcgatg gtcgtcgccg agacgcctga
     4021 caccgcgtat atccgtatcc cgaacctgac tgaacccggg tcacggccct tattcatcgc
     4081 ggaaggctca aaagtggtcg ctctgccggt ggcagtgggc gagttcaccc tcaagaacgg
     4141 agatagagag gtgcaaccag gtcaaacttt ggtgaccttt ccaactctgg agggcccgtc
     4201 cgacatttca gacgctgcct ggctgccggg aaacttcccc gtgacgaatc tggaggaggc
     4261 gcgcaagctg atacccggat tccagttgcc gcgagacgat caaaagcccc gccaggagcg
     4321 ggaagcgcaa caaaagcacg agtcaaaaga gaagcgggag gcggacgaga aaaaaggggg
     4381 atcgatcttg ctggtcgtca agaacctgac gcctgaacag gtttcgatgc gaggcaccaa
     4441 agaccagatg attgtcttcc atttgaagga tgcgtcgttc agccgagggg aattcaagta
     4501 cgacctcata gttgaggcca tcaagtccgg ccattttgat ataaggggct acgttattcc
//
LOCUS       DASRKK010000615         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1228352, whole genome shotgun sequence.
ACCESSION   DASRKK010000615 DASRKK010000000
VERSION     DASRKK010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228352"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="dnaX"
                     /locus_tag="VFC15_14840"
     CDS             complement(<1..629)
                     /gene="dnaX"
                     /locus_tag="VFC15_14840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZM11481.1"
                     /translation="MSYQVLARKYRPQKFSEVIGQEHVTRTLKNAIAQGRIAHGYIFS
                     GHRGIGKTTIARILAMALNCQSQDHPVPEPCGVCDACVEIRAGNAVDVIEIDAATNRG
                     IDEIRELREAARYRPARDRFKIYILDEAHQITDAAFNALLKTLEEPPSHIVFMMATTQ
                     PEDIPQTIRSRCQHFSFHAVRFDDIVQQLRDIARQEGIEADDNALAMLA"
     gene            complement(706..1632)
                     /locus_tag="VFC15_14845"
     CDS             complement(706..1632)
                     /locus_tag="VFC15_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZM11482.1"
                     /translation="MRKRGATIVEKAVAQFLRSLQERNASPHTIKAYRTDLAQFAEYV
                     GPQGWRDIDHVLIRGYLSRLYERGLSKTSVARALAALRSLYKWLAQEGEVEQNPAALV
                     ATPRLPKKLPRVPTIEEVNTVLDSAMPECSAFTERDQAILELLYGCGIRNSELIGINV
                     DDIRWSNELVLVRGKGRKERFVPFGDSAKAAVQAYLPARQRVLGETKRTTERALLINL
                     RGSRLTTRSVGRIVKQIVVARGLSPDVHPHTLRHAFGTHMLEEGADLRAIQEMLGHER
                     LSTTQRYTQLTVKHVMEVYDRTHPHAKGDRGG"
     gene            complement(1629..2528)
                     /gene="xerD"
                     /locus_tag="VFC15_14850"
                     /pseudo
     CDS             complement(1629..2528)
                     /gene="xerD"
                     /locus_tag="VFC15_14850"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
     gene            complement(2760..>3440)
                     /locus_tag="VFC15_14855"
     CDS             complement(2760..>3440)
                     /locus_tag="VFC15_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZM11483.1"
                     /translation="WKPDFYPKDVSSKNFLKYYATQLTAVEVNYTFRRQLSEKAATGW
                     MADVGPNFRFALKANQYITHIRRLQNVEEAIQRFLLTLQPLATQLGPVLFQLPPTAKA
                     DVAVLRDFLALLPANFKAAFEFRHQSWFSNEIYQALQDRNATLCVAETEKLNTPEVRT
                     ASYIYFRFRQPSYSADELKKLVDRVGRCVADGLETYAFFKHEEDPHSPHNAVEVLNSV
                     RKKLGGKS"
BASE COUNT          637 a         1047 c         1072 g          684 t
ORIGIN      
        1 tccgccagca tggccagcgc gttgtcgtcg gcctcaatgc cctcctgccg ggcgatatcg
       61 cgcagttgct gcacgatgtc gtcaaagcgc acagcgtgaa agctgaagtg ctggcagcgt
      121 gagcggatag tctgcggaat gtcctccggc tgggtcgtcg ccatcatgaa aacgatatgg
      181 ctgggcggct cttccagggt cttgagcagc gcattgaagg cggcgtccgt aatctggtgc
      241 gcctcgtcga gaatgtaaat cttgaagcgg tcgcgcgccg gacgatagcg cgcggcttcg
      301 cgcaactcgc gaatctcatc gatgccgcgg ttggtggccg cgtcgatctc gatcacgtcc
      361 accgcgttgc cggcgcggat ttcgacgcac gcatcgcaga cgccgcacgg ctcgggcaca
      421 ggatggtcct gcgactggca attcagcgcc atcgccaaaa tacgcgcgat ggtcgtcttg
      481 ccaatgccgc ggtggccgct aaagatgtag ccgtgggcga tgcgtccctg cgcgatggca
      541 ttcttcagcg tgcgggtgac gtgttcctgc ccgatcacct cggagaactt ttgtggacgg
      601 tatttgcgcg ccagaacctg ataactcatg gaaggggaaa acctgattat acggtgtggc
      661 gatgcggaac gggaatggcc ccggcccaca gtccggcgtc gggccttacc ctccccggtc
      721 tcccttcgcg tgcggatggg tgcggtcgta gacctccatc acgtgtttga cggtgagttg
      781 cgtgtagcgc tgcgtggtcg agagacgctc atggccgagc atctcctgga tggcgcgcaa
      841 gtcggcgcct tcctccagca tgtgcgtgcc aaaggcgtgg cgcagggtgt gcgggtgcac
      901 gtcgggcgag agtccgcgcg ccaccacgat ctgcttcacg atgcggccca cactgcgggt
      961 ggtcagccgc gatccgcgaa ggttgatcag caaagcacgc tcggtcgtgc gtttggtttc
     1021 gcccagcact cgctggcgcg ccggtaaata cgcttgcacc gccgccttcg ccgagtcgcc
     1081 gaacggcaca aagcgttcct tgcggccctt gcctcgtacc agaacgagtt cgttggacca
     1141 ccggatatcg tcaacgttga tgccgatgag ttccgagttg cggatgccgc agccgtagag
     1201 caattccagg atcgcctgat cgcgctcggt gaatgccgaa cactcgggca tggccgaatc
     1261 gaggactgtg ttcacctctt cgatggtggg cacgcgcggc agttttttcg gcagcctcgg
     1321 agtggccacc agcgccgccg gattctgctc aacctcgccc tcctgcgcca gccacttgta
     1381 cagcgaccgc aacgccgcca gcgcgcgcgc caccgacgtc ttgctcagtc cacgctcgta
     1441 cagcctggag agatagccgc gaatcagcac atggtcaatg tcgcgccagc cctgtgggcc
     1501 aacgtattcg gcgaactgcg ccaggtcggt gcggtaggcc ttgatggtgt gcggcgaagc
     1561 attgcgctcc tgcagcgaac gaaggaattg cgccacagct ttctcgacga tggttgctcc
     1621 gcgcttcctc attcgctctt ccctgcccgc ttgccccgcg gatgatgctc tttgtacacg
     1681 cgattcagac ggtcgagcgc cacatgggtg taaatctgcg tggtcgaaat gtcggcgtgt
     1741 ccaaggatgg tctgcaccgt gcgtaggtcg gcgccgttct ccaccatgtg agtggcgcaa
     1801 ctgtggcgca gcatgtgcgg gctggcgtgc cgcgccgact gagcggatga atcgttcacc
     1861 acctgccata cgcgctgccg tgtaatcggc cgcgccccgc gggcgatgaa caggaacgga
     1921 gacaacttct cagacgccag cactggacgg gcgtcctgca aatattgctg cacaacttcc
     1981 tgcgcggccc gccccagcgg aacgatgcgc tccttgtcac ccttgcctcg caccaggacg
     2041 taacccaact ccagcttcaa gtcttcgagc ttcaggctga tgacctcgga gacacgcagc
     2101 gcgccggcat agaaaacttc cagcatcgcc cgatcgcggc ccgtgagcgc tcgcgaccgg
     2161 cgatcatcct tggctcgagg tgggccttgc aacacgctct caacctcatc gacggccagc
     2221 gacttaggca ggaccttcca ttgccgcggc gaatcaatgt ttagcgtggg gtcggcggtg
     2281 atgtagcggt cgagcagcag gtacttgtaa aagtgccgca gtgcggaaag cttgcgcgcc
     2341 acgctgcgtc cgtcaacacc attggagagc agttcattaa ggaagtcgcg cacctcctgc
     2401 cgccgcgcct cgcgcagcgc gcgcttgcgc ttcagcagga actccgagta ctgctccaaa
     2461 tcagattcgt aagccgacac gctcagcggc gccagtccct tttccacctt gaggtagtcg
     2521 agaaaggagt tcagcagccg cagattggga tcgacactct gcacgagatg agtatgcgag
     2581 caatggcggc gcggtgggaa tagcgaggga ggtgcgggtg cgagcagaga aaatctgtta
     2641 ccacggagac acggagatca cggagatcac ggagaaagtt cgataaaacc aaaactccgt
     2701 gccctccgtg tcctccgtgg tgaatgattt tcgacggagc gcagtcgaaa cccatatcgt
     2761 cacgacttcc cgcccagctt cttcctcacc gagttcagca cctctaccgc attgtgcggg
     2821 ctgtgcgggt cttcctcatg cttgaagaac gcgtaggtct ccaggccatc ggcgacgcag
     2881 cgtcccaccc gatcgaccag cttcttcagt tcgtctgccg agtagctggg ctggcggaag
     2941 cgaaaataga tgtaactggc ggtccggacc tcgggcgtat tgagtttctc ggtttcggcg
     3001 acgcacaacg tcgcgttcct gtcttgcagg gcctggtaga tttcgttgct aaaccaagac
     3061 tggtgacgga actcgaatgc agccttgaag ttggccggca gcagcgccag gaagtcgcgc
     3121 agcacggcaa catccgcctt ggcggtgggc ggcaactgga agagcaccgg tcccagttgc
     3181 gtggccagcg gttgcagcgt gagaaggaat cgctgtatcg cctcttcgac gttctgtagc
     3241 cggcgaatgt gggtgatgta ctggttggcc tttagcgcaa accgaaagtt ggggccgaca
     3301 tccgccatcc atcccgtcgc ggccttctcc gacaactggc ggcggaaggt gtagttcacc
     3361 tcgacagcgg tgagttgcgt cgcgtaatac ttcagaaagt ttttcgatga gacgtccttg
     3421 ggatagaagt cgggcttcca
//
LOCUS       DASRKK010000616        22190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1033156, whole genome shotgun sequence.
ACCESSION   DASRKK010000616 DASRKK010000000
VERSION     DASRKK010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033156"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..2403
                     /locus_tag="VFC15_14860"
     CDS             373..2403
                     /locus_tag="VFC15_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11484.1"
                     /translation="MRRFSAARVLAALFAVLILSILPGCSGSSTTVSGVTTLILSPTS
                     LSMNTGQIATITATPQNSAGATVIADVTYTSSNTNLVTVTASGQVCAGIWDANFINCN
                     PVPGVAGIGSATITAVTANSVTATLPVTTHLQADRIVVDPIQGCVSMGTAPSYTAKVY
                     NTTSPGCSTYNPCDITSTVGTITFQSSDLQVMSNNITTGVLTATNPGATTIYATIGGL
                     NSLPEPALVCPIQSIKIHDAAGSNTSFTLAPAGTQSLTADVIDSNGVSVVPLLTWSSN
                     PMGSSTVTGASGAVTATVTAGASGTAIISAACSTPNCNRNVSPQYGQNVVTVTTTGSS
                     TTTAYAASTNSLSLVPIAIPGTTAGTTITLPFLPNSMVSNSAGNKIYIGSSSGLMTVD
                     PVATTVTSTIAAAGTVVAISPDGLFLLISNPAIPAVYILSTTSSTVILTQSVTPTSGV
                     FTPDSKSASFLIGQQVYYDTALPSATITNLPYVPAALDISSTGSLTYITSTASQAIDI
                     RSTCSQNDQQTLASNNPTLVAHIPNGTGAVVIDSPSIDIVATGSIGPGCPPLPQSTVN
                     TYPLNSGNLNPRQVLFSSDSSRAWIISDLTSVIGFNLSTLTPTTISLANGAQAYSGGI
                     TNDGSKIYLGASDNKVHVLDTASGADTAQIAVGLTDANSNAVAPNLVIVLPK"
     gene            complement(2488..2919)
                     /gene="nusB"
                     /locus_tag="VFC15_14865"
     CDS             complement(2488..2919)
                     /gene="nusB"
                     /locus_tag="VFC15_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZM11485.1"
                     /translation="MSKSGTRRKSRELALQMLFQLDMGKQTTDDVRKTFWAERRDLDE
                     KVRGFADDLFRVASEREAEIDQLIEKNAEHWRMDRMAAVDRNVLRAGIAEFLGFPDTP
                     RPVVINEALEIARRYSTPESVQFINGVLDSVARELGEAKAQ"
     gene            complement(2916..3776)
                     /gene="ribH"
                     /locus_tag="VFC15_14870"
     CDS             complement(2916..3776)
                     /gene="ribH"
                     /locus_tag="VFC15_14870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZM11486.1"
                     /translation="MIRNIIVPRVLGKSLDELQPALELFRALGASHGREWHEGADHGF
                     QLEAPGGVVELIAAANAPDTDVTLEVSDADAAWAIAKKRGVKVLREVADTAYGARLFA
                     VEVSGLRVAVLTYTNTSGMTAGVEGSLNAEGKRFAVVVSRFNSFITERLLDGAMLALR
                     QAGATENDITVVRVPGAFEIPSAARKLATSGKYDAIVCTGCLLRGDTLHYDVIANEVT
                     RGIGQSAQETGVPHAFGVLTCDTLEQAIDRAGLKAGNKGYEAGLAAVEMASLRDKIAE
                     PAVGATAGKR"
     gene            4378..5421
                     /locus_tag="VFC15_14875"
     CDS             4378..5421
                     /locus_tag="VFC15_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZM11487.1"
                     /translation="MNNCQRLLVILSPSSVSSTNVEDEVAFALEEHKTVIPVFYRDCK
                     VPFQLRPFQYVDFKTDYDRGLKTLLRTLGGEQQSAAAGGRAASAEPKDTAAHVSDAHE
                     RERAAASAAAQSDAKRKADEEKARQMEFEPKRLDRKKTPTPAPVRDAVRQKFPLWAKG
                     GVAIFALIVVGALVYWASLGSNPKQPVVEPQKQEAKSETRAPATTTPQVSIEQQAPPP
                     PSVAANAESSRPKTITQAKPGRFFEVIVRAKDSAWVSIKADGKITVRGIINPTDVKTV
                     HATEQIVFWTGNAGGVEVSFNGRPVPITGGPNEELLLEFNSHGLVSPPAADVKKPAAR
                     NCPEGPIATLTRT"
     gene            6256..6660
                     /locus_tag="VFC15_14880"
     CDS             6256..6660
                     /locus_tag="VFC15_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZM11488.1"
                     /translation="MNIDSEWDDNQPIYRQIRNFIVTTIMDGSLPEGEALASVRRLAA
                     EFRVSPLTVLKSYQQLVVEGLVESRRGRGMFVIPGARQLALAAERERFLQEEWPRVLD
                     TLLSLGLNPETLLKRAPRESGSEAKKPRLSHE"
     gene            complement(6699..7484)
                     /locus_tag="VFC15_14885"
     CDS             complement(6699..7484)
                     /locus_tag="VFC15_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZM11489.1"
                     /translation="MTDFQNLHLETKNHIAFITIDRPKVLNALNMATMEELCRAFVAA
                     KDDPEVRVVILTGGGEKAFVAGADIGELSKHSPVSAKEYTHRGQAVLDIIEDLGKPVI
                     ACINGFALGGGCELAMACTMRLASENAKLGQPEVKLGLIPGYGGTQRLSRLVGKGVAM
                     QLVLTGEMITAQEALRIGLVNEVVPAAELIPRAEAIAAKIIANAPLAVQYAMEAVNHG
                     LNMTLAEGLYLEATLFAVCCSTEDKNEGTKAFLEKRAAQFKGR"
     gene            complement(7514..7897)
                     /locus_tag="VFC15_14890"
     CDS             complement(7514..7897)
                     /locus_tag="VFC15_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11490.1"
                     /translation="MSVKNPLSAQEVNDVICKLAIPSNEVEVIFFDRETGIMFQTTGK
                     LSIWEGSLVIVHADVQSSTSETRFYATSLEKLQQTPAELADPEMLKYPPCAEFRPLDL
                     RVSFGVVFGFPNGSLLALLDVSSES"
     gene            complement(7914..8810)
                     /locus_tag="VFC15_14895"
     CDS             complement(7914..8810)
                     /locus_tag="VFC15_14895"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZM11491.1"
                     /translation="MDIKKVGVLGCGLMGSGIAQVAASAGCDVVVLEQDQKFLDKGFA
                     GIEKSLVRLVERGVDKGGITAEQKAAAQARLKGTTDIQDLADCDIVIEAIIENLDEKR
                     KTYAQLDSIVKKECIFASNTSSICITEMMTATKRPERFIGLHFFNPVPMMKLVEVVKT
                     IATAPEVYEAAVAFGTKLGKVPVRTSDKPGFIVNRLLVPYLLDAIRAYEEGVGSIPDI
                     DEAMKLGCGHPMGPFTLLDFVGLDTTYYITHVMYDEFKERRFAAPPLLKRLVLAGWYG
                     RKSGKGFYDYTDPKNPVPGKFV"
     gene            complement(8892..10097)
                     /locus_tag="VFC15_14900"
     CDS             complement(8892..10097)
                     /locus_tag="VFC15_14900"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZM11492.1"
                     /translation="MSNTFPEIAIVNGARTPMGRYCGNLRDFSAMDLGAIAAAEAMRR
                     SGVEPAEIDHCVMGNAQQTSSDAIYGARHVALKAGVPIEVPAITVNRLCGSGMQSVVT
                     AAQMIQLGESSIVLAGGMESMSQAPHVIRGMRWGVGLGEGKLEDSLTIMLFDTYCGLN
                     MANTAELYAEQQGITREMQDEYAVSSQKRADAAYKAGRIQEELTPVALRNRKGEPSGE
                     MFTEDDHRRPETTMESLAKLKASFAKNGTVTAGNASGIVDGGAAVVVMGLDDAVKRNL
                     KPLGRIVSWGIAGVEPNIMGSGPVPASRIALKKAGLTLSDIDLVEVNEAFAAQYLGVE
                     KELGLDRNKTNVNGGAIALGHPLGASGTRLLITVLYELRRRKARYGLSTACIGGGQGI
                     AVIVENLQR"
     gene            10385..10708
                     /locus_tag="VFC15_14905"
     CDS             10385..10708
                     /locus_tag="VFC15_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11493.1"
                     /translation="MDMHCSPPPNVFKHGLDVTGHSNANQWMAIVDAIVKLAADIPDV
                     SVSLQRDAQAAQCPEECDRKSIGKPILSGVSFAIGYDAKKNDWHCTIGVAGEIDVMCE
                     EPVKP"
     gene            complement(10789..11229)
                     /locus_tag="VFC15_14910"
     CDS             complement(10789..11229)
                     /locus_tag="VFC15_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM11494.1"
                     /translation="MASKERTRQLFSGPFGPQDFQQRLDEGWKPIAIEWEREVELSAA
                     TETTPAPEVPFGLQVAGDCSHLEENPTEREVLFQIMELTIQDGPYSGIAEELNRRGFR
                     TRHGNRWTPVSVFQMLPRLIEAGPGILNSEEWQQRRQRLKIAAR"
     gene            complement(11398..11634)
                     /locus_tag="VFC15_14915"
     CDS             complement(11398..11634)
                     /locus_tag="VFC15_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11495.1"
                     /translation="MKQVQPPTCPRCNSSDDVKQIASGLLPADQRPEAGNQGFVLGGR
                     CLRPESPDWYCSGCAESFGAGREFWGDPLTDLIQ"
     gene            complement(11645..13096)
                     /locus_tag="VFC15_14920"
     CDS             complement(11645..13096)
                     /locus_tag="VFC15_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM11496.1"
                     /translation="MSTSAIVAAVRQAAEKFPEKVALIAGEQQVSYSELIRRAQSAGA
                     HIAKQAEGENVGIFLPNSLDFAPSFLGALWAGKTVAVLPTLAPALLLKFMFAEARLTT
                     IFTSADLAPKLAEAGVPHVVIDIEYPTMSDFALQPRGHDTAVLLYTSGTTGRPKTVAL
                     SERNIISNAEGCRRATGFDDRQVMLAILPLFHAYGLTVTILLPLMSGSTVVISERFVP
                     RTVLNLIERHRVTTIVAVPSQYRVLAMDPTPCDASSLWLCIAGAERLPDTTEREFTTR
                     FGHAVLPGYGATELSPVVSLNIPETNRPASVGKPLTGTTVTIRNDDSSVCPVGEVGEI
                     CVEGPNVMLGYLNDPEGTARKIRNGVLYTGDKGFFDADGYLYISGRADEMVKVAGEKI
                     YPVEIENALERIAGVEEVAVVALHDPRGGVRLHAFVQRKPGAALDETSLRLACREVLE
                     PYKIPRTFTFVDSLPRTMTGKTDKRTLATSAPE"
     gene            complement(13247..14155)
                     /locus_tag="VFC15_14925"
     CDS             complement(13247..14155)
                     /locus_tag="VFC15_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11497.1"
                     /translation="MERRYLVATLALVATFAIFSREFRSGHLANLPCPRAALKAEIAC
                     ARQYLAEQFDAKIRPLVDRGIPDEQQMVAELNLPVLAAANERVAEVQAKIAEGVAEKQ
                     CDAAVRAQNNALRAQERSLRDVERMQERAAEISARAQERAMAMNERTTMRAQEISTRA
                     MERAQRAVEKSQWKMDKFQFKMIQPNAPLAPNAPVAPMPIDFEVNLPSDFSQQVQAAV
                     EGRVTVKCVRTKVAAQQYQTVMIQRGNQNMANNVHVVVSTQDVSGLTALTQSAKSQTA
                     LRRIERDVQRFQDHIMRSVDRAFATL"
     gene            14447..14947
                     /locus_tag="VFC15_14930"
     CDS             14447..14947
                     /locus_tag="VFC15_14930"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM11498.1"
                     /translation="MTAEEVKELLQLTPLELEGGFFRETYRSRWQVWAEYLPEGTHGS
                     RSIGTAIYYMITPESFSTLHRLPGTEVFHFYMGDPVVMLQLHPDGASRTVTLGTDLAS
                     GQEPQVVVRGNVWQGCRLAPGGKWALLGTTMSPGFDYADYVTGDRDELIAQYPEVAEM
                     IRKYTR"
     gene            complement(14972..15211)
                     /locus_tag="VFC15_14935"
     CDS             complement(14972..15211)
                     /locus_tag="VFC15_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11499.1"
                     /translation="MMLLPDHERFNTSCETLCTALRWKQQFIGVDQDPTVPPTNDGLF
                     WCLHTQTCIGPDGQLAEPGNCSSPGRKCHGTGKCG"
     gene            complement(15208..16230)
                     /locus_tag="VFC15_14940"
     CDS             complement(15208..16230)
                     /locus_tag="VFC15_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZM11500.1"
                     /translation="MQAFEYASASKKEQVAALIGDQGAILAGGTDLLALMKDDVVTPK
                     RLVNVKHIDGLRGITYQPGNGLRIGALTTIAEFADDATVRQHYPVLAEAAIEAASPQI
                     RNLATMGGNMCQRPRCWYFRNGMGLLPKAPNGKSTVVEGDNRYAAILGNDGPAYFVSP
                     STIAPVLIAYGAKIRIYSAKASGSGVRELPLEKFFVIPTADGQREHDLKLDEMVIELI
                     VPPPPQNLKAASYEVRQRAAFDWPLAMASVALEMDGNAVKSARVVLGHVAPIPWISPE
                     VAQALVGKTITPDTAMAAANAAVAQAKPLSRNKYKITLTKAAVKRAILNAANGDSGSS
                     IFGGAA"
     gene            complement(16217..18586)
                     /locus_tag="VFC15_14945"
     CDS             complement(16217..18586)
                     /locus_tag="VFC15_14945"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZM11501.1"
                     /translation="MSQYSWPDPATRPLLGKKIDRVDGPSKVSGKAKYTYDYNPQGLL
                     AGKILRCPYAHAKIKSIDTSAAEKMPGVKSVVIIQKPGTEIFWAGDEIVGVAAVDEGT
                     VEDALRAIKVEYEVLPHFVSDAEPPKNISTDSGPLSNDDFQDMEENQVPDEEVIAAIQ
                     KRGISFKPDEKDYESMKGMGVEPAVIAALRKAKFVPAEAKPKSPYKKTGLQTTGTPDK
                     AFASAAAVVEGVYGVPCIVHVCLETHGSAMEWPAEKEVVVHVSTQNVSGIAAQLAEPL
                     GVPAGNINVLQQNVGGGFGSKFSVDRWDVNTANLSRKSAGKPVKNMLDRRAEFEVAGM
                     RPSAYARVKIAAAKDGKIVAWDSRSWGTGGVGGGGTPPLPYIFEIPHQRKQNTAISTN
                     QGSARAWRAPNHPQAAVITMGAIEDLAAKMNLDPLEFISRNLDIAGPRAQTYREEFAI
                     ADDLMGWKKNWHQRGDKTPGPIKRGLGLSMHTWGGRGHNSNCDLTIHPDGSVEIKMGT
                     QDLGTGARTIITIVAADTLGIPMESVNLLYGNNRYPQSGGSGGSTTSGGVTASTRRAA
                     VDARDQLCAKVAPALKAQPDELEAADGTVRVKADPSRSLPWKQACAKIGAVPLTVHGS
                     NPDKAKPPDLTNSGVGGVQMADVSVDVETGIVRINKMVAVQDCGLIVDMKTAMSQVYG
                     GLIMGISYALYEEKVLDPVSGRVLNPNLEFYRLAGLSDIGELKVHMMTGKGYDERGVI
                     GLAEPAVVSPGAAISNAVANAIGVRVPFLPLTPDRVLEALEQGGAHASV"
     gene            complement(18583..19206)
                     /locus_tag="VFC15_14950"
     CDS             complement(18583..19206)
                     /locus_tag="VFC15_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM11502.1"
                     /translation="MKDDDARQPDSTSGVSRRDFMKISAVTAAVPLVLGPKVVEAAGE
                     DVTVHGPGKVLVALNVNGKKLTADLEPRVTLLDALREQFDLTGAKRVCDRGACGACTV
                     LLDGKPVYACSVLAIDAQGTKITTIESMAEGDALSPVMQAFVAHDAQQCGFCTPGFVV
                     ATTHFLQQNPKPSAEDIRHGLSGNYCRCGTYDGIRAVALAHAGGKQS"
     gene            complement(19481..19801)
                     /locus_tag="VFC15_14955"
     CDS             complement(19481..19801)
                     /locus_tag="VFC15_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM11503.1"
                     /translation="MSLVKKIALVGLALALAVPMIATAQGTGADTFKAKCAMCHGADG
                     SASTGMGKSMGLKPLGSPDVQKISDADMSALITNGKGKMPAFKGKLTDAQITDVVKYV
                     HTLK"
     gene            20110..21408
                     /gene="hemA"
                     /locus_tag="VFC15_14960"
     CDS             20110..21408
                     /gene="hemA"
                     /locus_tag="VFC15_14960"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZM11504.1"
                     /translation="MEPTLAVIGLNFRTSPVAIRERFWIAEPRRYETLHQLVRSEGIE
                     EVAVLATCNRTEFIVWANDVPEASNSVLRFLTQEYQLKLCEWSHFYRLMDDAALTHIF
                     QVAAGLDSMVLGEPEIAAHLREALQQAQKSGTSGRFLDGIIQKALAVSARVRSETAIA
                     DSNLTVPFASVELSIDVLGDLSGCEVLLVGAGKVSELAAKCLRSAGATRIKVMNRTQA
                     NAEELALKLNAKAVSCEDRLSHLKSADIVVCSTSCPHSIISLEDAQHIARARQHKPMV
                     MIDMAVPRDIDPRVGEVDGIHLFDMDDLERVVKRDIRERRAAADEANEIVVHEVLGFR
                     RKLMVERVIPTVVALRRRLEELCHEELEFVRKEYGPFTDDQDQILTTLAAHITQRLAG
                     SLARELRELPDRTGQEMLTVAVHRLFQLEPSVMGEAGKEN"
     gene            21440..22138
                     /locus_tag="VFC15_14965"
     CDS             21440..22138
                     /locus_tag="VFC15_14965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11505.1"
                     /translation="MPESAQDKTNTTLGFTSVQAYTLAVITLVIGIAVGYFARGSAPA
                     AVAPEAAQTAAATAPAGMGGRAGMGAGQLPGIGSQQQGGSSPEMLAQAAQPLLTKLQA
                     TPKDFETLRQLGNLYYDGQAFPQAIEYYNKALTIDPKNPDVLTDVGTAYWYSGDADKA
                     LAEFGKSLAVRPNHPGTMFNEGIVMWQGKKDPKGAVVVWEQLLKTNPNYEQKEQVQML
                     IERAKMHGAGATKG"
BASE COUNT         4382 a         6651 c         6466 g         4691 t
ORIGIN      
        1 ttcccacatt cccaccgccc cgacggctgc tagactcgtt caatacaaat caggaaagga
       61 gctttcctcc gccatcccct cgggcttact tcaggctcat tcttcaattg gaaaagactt
      121 ccattccggc gcaaaaaatc aatgcgccgc agccacatcg ctgttccgct ctctcctcac
      181 aactcatccg tggaatccgt gtttgcagaa ttcggcggct gtccgcgctg ctctcgcaga
      241 ataccgtgaa aacgcagcct ttgggtcgcg gcttgccgaa ttgtgggtca gcttctacac
      301 tgagaaattc tggcaggatt cgcattttgg gtctttatac atccgtcgga accgacccgg
      361 cggggagggt gcatgcgtcg tttttcagcg gctcgagttt tggctgcctt gtttgcggtc
      421 cttatcctaa gtattctgcc cggttgtagc ggctccagca cgactgtcag cggcgtaacc
      481 acgctcattc tcagtccgac ttcgctctcc atgaacaccg gacagatcgc caccatcacg
      541 gcgaccccgc agaattcggc gggagccact gtcatcgccg atgtcaccta caccagcagc
      601 aacacgaatc tggtgacggt tacggctagt ggacaagtct gcgccggaat ctgggacgcc
      661 aatttcatta actgcaatcc ggttcccggc gtggccggga taggatcagc gaccatcacc
      721 gccgtcacgg caaattccgt cactgccacc ttgccggtta ccacccacct gcaagccgac
      781 cgcattgtcg tggatccgat acagggttgc gtgagcatgg gcaccgctcc ctcttacacc
      841 gccaaggtct acaacaccac ctcgccggga tgttccactt acaatccctg cgacattact
      901 tcgacagtcg gcaccatcac tttccagagc tctgatcttc aagtcatgag caacaacatc
      961 accacgggag tgctgactgc gaccaacccc ggtgctacca caatctatgc caccatcggc
     1021 ggcctcaaca gccttcctga acctgcactg gtctgcccga ttcaatccat caaaattcac
     1081 gatgccgctg gctcaaacac atctttcact ctcgcgccag ccgggacaca gtccctcacc
     1141 gccgatgtca tcgacagcaa tggcgtttcc gttgtcccgc tgttgacctg gtcttcgaac
     1201 ccgatgggca gctccacggt gaccggagct tccggcgccg tgaccgctac agtcaccgcc
     1261 ggcgcttccg gaaccgccat catctccgcg gcttgcagca ccccgaattg caatcggaat
     1321 gtcagtccgc aatacggcca gaacgtagtt acggtcacca ccaccggctc ttccaccaca
     1381 acggcttacg cggccagcac caattccttg tcactggtac ccattgccat ccccgggacc
     1441 accgcgggga ccacaattac gctgcccttc cttcccaatt ccatggtctc caactccgcc
     1501 ggaaacaaaa tatacatcgg atcaagttcc ggtttgatga ccgtggatcc ggtggcaact
     1561 accgtgacct ctacgattgc cgcagccgga accgtggtgg ctatctcccc cgacggcctc
     1621 ttcctgttga tctcgaaccc cgctataccc gctgtttaca ttcttagtac tacgtcgtca
     1681 accgtcatat tgacgcaatc ggttacaccc acttccggcg tctttacgcc tgatagcaag
     1741 tcggcttcct tcctcatcgg gcagcaggtc tactacgata cggcgttgcc ctcggcaacc
     1801 attacgaatc tgccctacgt tcccgccgct ctggacatca gctctaccgg cagtctgact
     1861 tacatcacca gtacggcgtc gcaagccatc gatatccgct cgacctgcag ccagaacgac
     1921 cagcagacgc tggcctcgaa caatcccacc ctggttgccc atattccaaa cggtaccggc
     1981 gcggtggtca tcgattctcc ttcgattgac atcgttgcga ctggatcgat cggacctggt
     2041 tgtccgccgc ttccccagag cacggtgaat acctacccgt tgaacagtgg caacctgaat
     2101 ccccggcagg ttttgttttc gtcggacagc tcgcgcgcct ggatcatttc tgacttgacc
     2161 tccgtcatcg gctttaacct gtctactttg acgccaacca ccatttcgct ggcaaacgga
     2221 gctcaggcct acagcggcgg cattaccaac gatggttcta agatctatct cggcgccagt
     2281 gacaacaagg tgcacgtgct ggacacggct tcgggcgcag atacggcaca gatcgccgtc
     2341 ggtttgacgg acgccaactc caacgcagtc gctcccaacc tcgtgattgt gctgcccaaa
     2401 taaaccggcg ttccctacca aagaaaacgc cctcccatcg cggagggcat tttcttttct
     2461 gcaagtcttc cggatttggc cgcgcggcta ttgcgctttc gcttctccca actcgcgcgc
     2521 cacactgtcc agcactccat taatgaactg cactgactcc ggagtggaat atcgtcgcgc
     2581 gatttccaac gcctcgttaa tgaccaccgg acgaggcgtg tcggggaatc ccagaaactc
     2641 ggcaatcccc gcacgcagca cattgcgatc cacggcggcc atgcggtcca tgcgccaatg
     2701 ctccgcgttc ttctcgatca gttgatcgat ctcggcttcg cgctctgacg ccacacgaaa
     2761 caaatcgtcc gcgaagccac gcaccttttc gtccaggtca cggcgttcgg cccaaaaagt
     2821 cttgcgcacg tcgtcggtcg tctgtttgcc catgtcgagc tgaaacagca tctgcagcgc
     2881 cagttcacga gacttcctgc gcgttccgga cttgctcatc gcttccccgc cgtcgcgccg
     2941 acagccggct cagcaatttt gtcgcggagt gaagccattt ccaccgctgc caatccagcc
     3001 tcgtagccct tgttgccggc cttcaaacct gcgcggtcga tggcctgctc caacgtgtcg
     3061 cacgtcagaa ctccgaaagc gtgcggcacg ccagtctcct gcgccgactg cccaatcccg
     3121 cgcgtgacct cgttggcaat cacgtcgtag tgcagagtat cgccgcgcaa caggcatccg
     3181 gtacagacga tcgcgtcgta ttttccgctc gtggccaact tccgcgctgc cgacggaatc
     3241 tcaaacgctc ccggcacgcg cacgactgtg atgtcgttct cggtcgcacc ggcctgacgt
     3301 agcgccagca tcgcgccatc aagcaaccgt tccgtgatga acgaattgaa gcggctcacc
     3361 accacggcaa accgtttgcc ctcggcgttc agcgagcctt cgacgcccgc ggtcattcca
     3421 ctcgtattcg tatatgtaag gaccgcgact cgcagcccgc tgacttcgac cgcaaacaga
     3481 cgcgcgccgt aagccgtatc cgcaacctcg cgcagaactt tgacgccacg cttcttggcg
     3541 atcgcccatg cggcatcagc gtcagaaacc tcgagggtca cgtcagtgtc cggagcgttc
     3601 gccgcggcaa tcaactccac cacaccaccc ggagcctcca attggaaccc gtggtccgcg
     3661 ccctcatgcc actcgcgacc atgtgacgca cccagggctc gaaacaactc caaagccggc
     3721 tgcaactcgt caagcgactt ccccagcacc cgcggaacaa taatatttcg gatcataagc
     3781 tctcaattgt attcgggaat tcgggatcgc gtcggtgacg atggaaccag gacactgggt
     3841 gccccgttct accgctcgcg cagcgaacgg tagggccggg tctttcgttc ccctcgcaag
     3901 aaatctgggt gccccattca aggccctctt ttgcccttga gtggggattt tccccgccac
     3961 ggcgaaagac taaaccaatg gcccacatct cttagaaacg gggtggcccg catttccccg
     4021 attttggcaa atgcgggaac gagaatgcag cgccggaggc gcgatagagc ttagccccgc
     4081 ctttcaaggc ggggtaacgt cgtcactgaa agtcagagtc ccgccaggga cggcagaagc
     4141 aaattggacg ggcttaagcc cgtccacaac atctgtcgtg cctgccgggt ggcgagttcc
     4201 accgttcgcg cagtgaacgg tagggcgggg tctttcgttc tcgtctcacg aaatctggga
     4261 gcccctccac ttgtttgtca cccccccgcc tccgccgcgg aggcgcgagg gatctgcttt
     4321 cccttcttcc acgaaactcc aggttttgag gactcaattc cccccatcca ggatgccttg
     4381 aacaactgcc agcgcttgct ggtgattctg tctccctctt ccgtcagctc caccaacgtc
     4441 gaggacgaag tcgccttcgc tcttgaggaa cataagaccg tcattccggt tttctatcgt
     4501 gattgcaagg ttccttttca actgcgtccc tttcaatatg tcgatttcaa gaccgactat
     4561 gaccgcgggc tgaagacact gctcagaaca ttgggtggag agcagcaatc cgccgctgcc
     4621 ggtggtcgcg cagcttccgc cgagccgaaa gacaccgcag cccatgtctc ggatgcgcac
     4681 gagcgcgaac gggcggcggc cagcgcggct gcgcagtcag atgcgaagcg caaggccgac
     4741 gaggaaaagg ctcgccagat ggaatttgag cctaagcggc tggacaggaa gaaaacgccg
     4801 actcccgcac cagtgcgaga cgctgtccgt cagaagtttc cgctttgggc gaagggtgga
     4861 gtcgccatct tcgcgctaat tgtcgtcggg gcgcttgtgt attgggcatc actcgggtcg
     4921 aacccgaagc aaccagtggt agagccgcag aaacaagagg caaagtcgga aacacgcgct
     4981 cccgcgacga ccaccccaca agtttcaatc gagcagcaag ctccgccgcc cccaagtgtc
     5041 gcggctaacg ccgagtcctc ccgacccaag acgattaccc aggcgaagcc cggtcgcttt
     5101 tttgaggtaa ttgtgcgcgc aaaggatagc gcctgggtct cgataaaggc ggatggcaaa
     5161 ataacggtgc gtggcatcat taaccccaca gacgtgaaga cagtccatgc caccgagcag
     5221 attgtcttct ggaccggcaa tgcgggcggc gtagaagttt cgttcaatgg gagacccgtt
     5281 ccaataactg gcgggcccaa tgaggaattg ttacttgagt tcaactctca cggattggtg
     5341 tcgccaccgg cggccgatgt caaaaagcca gcggccagaa actgccctga ggggcctatt
     5401 gcgaccctga cacgaaccta atgtggacga tgaaagataa cggtaaagac atcacttggc
     5461 ccgacgcaga acaacactgc aagagcctga ctttggccgg attatcgggt tgggaactgc
     5521 ccacgatcga cgaactggaa aagctctacg atcgccaaag ctcaaacgaa tacaaaatcc
     5581 gaaaaccgct ccgactgacc acatgttgcc cgtggagttc gacgaagcaa ggctggggtt
     5641 ccgcgtggag cttcaacttc ggcagcggta ggcggggcgt cttccccagt aacgtttccg
     5701 tcggcggccg cgccttgtgt gcgcgtcgtt ccggaaaatg atgtgggcga tttgggcagg
     5761 tagtcggtgg tcgggtgccc catccggcct gattgctcct gcattgattt gtcgtatccg
     5821 tgtgatccgt gtaaatctgc ggttgctttt gttcttctcg atttaccact cgcgttgttc
     5881 atccatcgcc acgcacaatc gaggtcccat attgacactc cacaggttac tttcgttccg
     5941 ccggaagccc gaatctcctt gcgcgccact cgctgcctcc tctatacctt caccgaggac
     6001 cgaggaattc ctgtgccctc acctcgcgac ctaggccgcg attgccgacc gcgctttgac
     6061 aactcgacat catcggcctt gctggccgct ccttgatttt gagagctttt cggatgtgac
     6121 atggtgttat agctatgtat aacaacttaa gaaatttacg ataacactgt aagccattta
     6181 tttatagtga cctattgcgg ctgagtatgt gttatagtta cgtataacaa caaaagaaat
     6241 gagggatctc gactgatgaa catcgactct gagtgggacg acaatcagcc catttaccgc
     6301 caaatccgga actttatagt gaccacgatc atggacggaa gcctgccgga aggcgaagct
     6361 ttggcatccg tccgccgtct agcggccgag tttcgcgtga gtcctctcac cgtcctgaag
     6421 agttatcagc agctggtagt cgaagggctg gtcgaatcgc gtcgaggacg cggcatgttc
     6481 gtcattccgg gcgcccgcca actagcgctc gcggccgaac gcgaaaggtt tttacaggag
     6541 gaatggccgc gtgttttgga tacccttctt agcctcggcc tcaaccccga aacactactg
     6601 aagcgcgcgc cacgagagtc aggatctgag gccaagaaac cgcgattgtc ccacgagtag
     6661 tggcatccaa ttcttttcta accactagcc actatccact atcttccctt aaactgcgct
     6721 gccctcttct ccaggaacgc cttcgtgcct tcgttcttgt cctcggtcga gcagcacacg
     6781 gcgaagagcg tggcctcgag gtagaggcct tcggctaggg tcatgttgag gccgtgattc
     6841 accgcttcca tggcgtactg caccgccagc ggagcgttgg ctatgatctt ggccgcaatc
     6901 gcttcggcgc gcgggatgag ttcggcggcg ggaacgactt cgttcaccag cccgatgcgc
     6961 aatgcttcct gcgccgtgat catctcgccg gtcagcacca gctgcattgc cacgcctttg
     7021 cccaccagtc gcgacaaacg ctgcgtgcca ccgtagccgg ggattaatcc tagtttcact
     7081 tccggctgac cgagcttggc attttcgctg gccaggcgca tggtgcaggc catcgccagt
     7141 tcgcatcccc cgccgagggc gaagccattg atgcaggcga tgaccggctt gccgaggtct
     7201 tcgatgatgt cgaggacagc ctggccgcgg tgcgtgtatt ccttggccga aaccggcgag
     7261 tgcttcgaca gttcgccaat atcggcgccg gcgacaaagg ccttttcgcc gccgccggtg
     7321 aggatgacca cgcggacttc ggggtcgtcc ttcgcggcga cgaaagcgcg acagagttcc
     7381 tccatggtgg ccatgttgag ggcattgagc accttggggc ggtcaatggt gatgaaggcg
     7441 atgtggtttt tcgtttcgag atggaggttt tggaagtcgg tcatgatttc ctcattaagc
     7501 tattaaataa cgattaggat tcagagctca catctaagag agccagtaat gagccgttgg
     7561 gaaacccaaa aaccacgccg aacgagactc gtaaatcgag cgggcggaat tcagcgcatg
     7621 gtggatattt caacatctcc gggtcagcta attcggcagg agtttgctgg agcttttcga
     7681 gcgacgtggc atagaaccga gtctcagatg ttgatgactg gacgtcagcg tgcacaatga
     7741 ctagcgatcc ctcccaaatg ctgagcttcc cagttgtctg gaacatgatg cctgtttccc
     7801 ggtcaaaaaa gataacttct acctcattcg acgggatggc taacttgcat atcacgtcat
     7861 tgacttcttg tgccgacaat gggtttttga cactcatgcg cctcaactca cctctacaca
     7921 aactttcccg gcacggggtt cttcgggtcg gtgtaatcgt agaagccctt gcccgacttg
     7981 cggccgtacc agccggccag caccaggcgc ttcagcagcg gaggcgcggc aaagcgccgc
     8041 tccttgaact cgtcgtacat gacgtgcgtg atgtagtacg tggtgtcgag cccgacgaaa
     8101 tccagcaggg tgaacgggcc catggggtgg ccgcagccca gcttcatggc ctcgtcgatg
     8161 tcagggatcg agcccacgcc ctcttcataa gcgcggatgg cgtcgagcag atacggcacc
     8221 agcaggcggt tgacgatgaa tcccggcttg tcgctggtgc gcaccgggac cttgcccagc
     8281 ttcgtgccga acgcgaccgc cgcttcgtaa acttcgggag cggtggcgat ggtcttcacc
     8341 acctcgacca gcttcatcat ggggacagga ttgaaaaagt gcagcccgat gaagcgttcc
     8401 gggcgcttgg tcgccgtcat catctcggtg atgcagatgg acgaggtatt gctggcaaag
     8461 atgcactctt tcttaacgat cgaatcgagt tgcgcgtaag tcttgcgctt ctcgtcgagg
     8521 ttttcgatga tcgcctcgat cacgatgtcg cagtccgcca ggtcttgtat gtcggtggtc
     8581 cccttcaacc ttgcctgcgc cgctgccttc tgctcggccg tgatgccgcc cttgtctacg
     8641 ccgcgctcga ccaggcgcac gagcgatttc tcaatccctg cgaaaccttt gtcgagaaac
     8701 ttctggtctt gctccagcac caccacgtcg caacccgccg aggccgcgac ctgtgcgatg
     8761 cccgaaccca tgagaccaca acccagtacg ccaacttttt tgatatccat aaggacctcg
     8821 aaatagtttc gagtttcaag cttcaagttt cgagtaaaac caattttcag aacttgaaat
     8881 tcgaaacact tttatctctg caagttctcg acgatgacgg caatgccttg cccgccgccg
     8941 atgcacgcgg tcgaaagtcc gtagcgcgct ttgcgcctgc gcaattcgta gagcaccgtg
     9001 atcagcaacc gcgtcccgct cgcgccgagc ggatggccga gcgcaatcgc tccaccgttc
     9061 acattggtct tgttgcgatc gagtcccaac tccttttcga ccccaagata ctgcgctgcg
     9121 aaagcctcat tcacttcgac gagatcgatg tctgacaatg tcagaccagc cttcttcagc
     9181 gcgatgcggc ttgccggaac cggccccgat cccatgatgt tcggctcgac cccggcaatg
     9241 ccccaactca caatccgtcc caacggcttg aggttgcgct tcactgcgtc atcgagcccc
     9301 attaccacga ccgccgcgcc gccatccacg attccgctgg cgttgcccgc ggtgaccgtt
     9361 ccgttcttgg caaacgacgc tttcagtttg gccaggctct ccatcgtggt ctccggacgc
     9421 cgatggtcgt cctcggtaaa catctcgccc gacggctcgc ccttgcgatt gcgcagtgcc
     9481 actggcgtca gctcttcctg gatgcgaccc gccttatacg ccgcgtccgc tcgcttctgc
     9541 gacgacaccg cgtactcatc ctgcatctcg cgtgtaatgc cctgctgttc ggcgtagagt
     9601 tcggcggtat tggccatatt cagaccgcaa taggtgtcga acaacatgat cgtcagcgag
     9661 tcttccagct tgccttcgcc cagcccgacg ccccagcgca tgccgcgaat gacgtgtggc
     9721 gcctgagaca tcgactccat gcctccagcc agcacgatcg acgactcgcc taactggatc
     9781 atctgcgccg cggtcactac cgactgcatt cccgagccgc acagccgatt cacggttatg
     9841 gccggcactt cgatgggcac tcccgctttc agggccacat gccgcgcccc ataaatcgcg
     9901 tcggacgacg tctgctgagc gttgcccatc acgcagtgat cgatctccgc gggctcgact
     9961 cccgagcgcc gcatcgcttc cgcggcggca atcgcgccca ggtccatggc ggagaagtcg
    10021 cgcagattcc cgcagtagcg tcccatgggc gtgcgcgccc cgttgacaat tgcgatttca
    10081 ggaaatgtat tcgacatggc tggaaggagt ggccccggtg actgtctgag cgaacggaca
    10141 agtctaccag cgctccgaga gtgccgcaaa tgctctaacg gagtgggagc aggggaacct
    10201 cggttggatg gatcagtggg ctacgggatt cgctgactta gctggtagct tggacataca
    10261 cttcagcacg acgccgtctg gggactcact tgagagaaaa aaggttgttt tccaagagga
    10321 accgaagcac aattccccgc gaatcgggac aaaatgaaga ttccggctga aaggattccc
    10381 gcgtatggac atgcactgtt cgccaccacc gaacgtattc aaacacgggc tggacgtcac
    10441 cggtcacagc aacgcgaacc aatggatggc aatagtagat gccatcgtca aactcgcagc
    10501 tgacattccg gacgtttcag tgtcacttca gcgcgatgcg caggccgctc aatgccctga
    10561 agagtgcgat cgaaagtcga tcggcaaacc gatcttaagt ggtgtcagct tcgccatcgg
    10621 ctacgacgct aagaaaaatg actggcattg cactattgga gtcgcaggtg aaatcgacgt
    10681 gatgtgcgaa gagccggtca aaccataaaa ccggcggttg gcccgggcga gcgttatccg
    10741 atttgggtga caactggtta gaaggtgcga cggttccgca ggattacctt acctggcggc
    10801 gatcttgagg cgttggcggc gttgctgcca ctcttcgctg ttgaggattc caggcccggc
    10861 ttcgatcagg cgaggcagca tctggaaaac agacacgggt gtccagcggt tgccatggcg
    10921 ggtgcggaag ccgcgccggt tcagctcttc ggcgatgccc gagtagggac cgtcttggat
    10981 ggtcagttcc atgatctgga acagcacctc gcgctccgtc gggttctctt ccaggtgcga
    11041 gcagtcgccg gccacctgca ggccgaaggg aacctcgggc gcaggcgtcg tctcagtagc
    11101 agccgaaagt tcaacctcgc gctcccattc aatggcgatc ggtttccatc cttcgtcgag
    11161 gcgttgctgg aaatcttgcg gcccaaacgg gcccgaaaat agctggcgcg tgcgctcttt
    11221 gctggccatc gttcccctat cctctcgcgg atcttcgcga gtgataagag cagttggcgt
    11281 gccagaaagg atgacgcgga tgtaactgat gcagcgcagg ttgcgcgcga tgggtcacga
    11341 tggggcgggc aggcgatgtc cgttggcagg acgaagtgtg cggaattggg cagccggcta
    11401 ctggatcagg tcggtcagcg gatcgcccca aaattcgcgt ccggcgccga aactctcagc
    11461 gcagcccgag cagtaccagt caggactttc ggggcggagg cagcgcccgc cgagcacgaa
    11521 gccttgattg cccgcctcgg gacgctggtc tgccggcaac agtccggacg cgatttgctt
    11581 tacgtcatcg ctgctgttgc accgtggaca ggttggaggc tggacttgct tcatgagaca
    11641 cctcttactc gggggccgaa gtggcgagtg tacgcttgtc ggtcttgccc gtcatggtgc
    11701 gcggcaaact atcgacaaag gtgaaggtgc gcggaatctt gtagggttcc agaacctcgc
    11761 ggcaagccag tcgcaggctg gtctcgtcga gcgccgctcc cggcttgcgt tgtacgaagg
    11821 cgtgcagacg aactccgccg cgcggatcgt gcagcgcgac caccgcaacc tcttccacac
    11881 ctgcgatgcg ctcgagcgcg ttttcgatct ccacgggata gattttctcg ccagccacct
    11941 tcaccatctc gtcggcgcgg ccggaaatgt agagataacc gtcggcgtcg aagaatccct
    12001 tgtctccggt gtagagcacg ccgttgcgaa tcttgcgcgc cgtgccttcg ggatcgttga
    12061 ggtatcccag catgacgttg gggccctcga cacagatctc gccaacttcg ccgaccggac
    12121 acacggagct gtcgtcattg cgaatcgtca ccgtggttcc ggttagcggc ttgcccacgc
    12181 tggcgggacg attggtttcc ggaatgttga gcgagactac aggcgagagt tctgtcgcac
    12241 cgtatccggg aaggacggcg tgtccgaagc gggtggtgaa ttcgcgttcg gtggtgtcgg
    12301 gcaggcgctc ggctccggcg atgcagagcc acaacgaact ggcgtcacag ggagtgggat
    12361 ccatcgccag cacgcgatat tggctaggca ccgcgacgat ggtggttacg cgatgacgct
    12421 cgatcaggtt gaggacggtg cgaggcacga agcgttcgct gatcaccacg gtcgatccgc
    12481 tcatcagcgg cagcaggatg gtgacggtca ggccgtaggc gtgaaacagc ggcaggatgg
    12541 ccagcatgac ctggcgatcg tcgaagccgg tcgcccggcg gcatccttcg gcgttggaaa
    12601 tgatgttgcg ttcgcttagg gcaacagttt tcgggcgtcc ggtggtgccc gaggtgtaga
    12661 gcagcaccgc cgtatcgtga ccgcggggct ggagagcaaa gtctgacatt gtgggatatt
    12721 cgatgtcgat gacaacgtgc ggcactccgg cttcggccag cttgggggcg aggtccgcgg
    12781 aagtgaagat ggtcgtcaat cgcgcctcgg cgaacatgaa tttcagcagc agcgccggcg
    12841 ccagcgtggg cagcacggca acggtctttc cggcccagag cgctcccaga aaactcggcg
    12901 caaagtcgag tgagttgggg agaaagattc cgacgttctc gccttcagcc tgcttcgcga
    12961 tgtgcgcgcc tgcggactgc gcccgccgta tcaactcgga atagctgacc tgttgctcgc
    13021 ccgcgatcag agctactttc tcgggaaatt tttcggcagc ttggcgtacc gccgcaacga
    13081 ttgcgctggt gctcatgctt cacctaatag tggaaagaat gcgacggtgg ggagtgtaca
    13141 cctttgtgac aggaaaggct gtgagaacga tcagtggagt tgatgcacga gcagaatcag
    13201 aaaatgctgc ggggtattca ccgcagcata atcatggtaa cgaggactag agcgtagcaa
    13261 acgcgcgatc gacggagcgc atgatgtggt cctggaagcg ctgaacgtcg cgctcaatcc
    13321 gacgcagcgc ggtctgcgac ttcgcactct gtgtcagtgc agtcaagcct gagacatcct
    13381 gagtggaaac caccacgtgc acattgttgg ccatgttctg gttgccgcgc tggatcatga
    13441 ccgtctggta ttgctgagcg gcaactttcg tacgtacaca tttcactgtg acgcggcctt
    13501 cgacagcggc ctgcacctgc tgactaaagt cagacggcaa gttgacctcg aaatcaattg
    13561 gcattggtgc gactggcgcg ttgggcgcta gaggcgcatt aggctggatc atcttgaact
    13621 ggaatttgtc catcttccat tgggactttt ccacggcccg ctgcgcccgc tccatagcgc
    13681 gtgtgctgat ttcctgcgcc cgcatcgtgg tccgctcgtt cattgccatc gcgcgttcct
    13741 gagccctggc gctgatttcc gctgctcgct cctgcatgcg ttccacgtcg cggagactgc
    13801 gctcctgggc ccgaagggcg ttgttctgtg cacggacggc ggcgtcacac tgcttttcgg
    13861 caacgccttc cgcaatcttc gcctgcactt cagcaactcg ctcattggct gcggccagca
    13921 ccggcagatt caactccgcc accatttgct gttcatctgg aatgccgcgg tcgaccaagg
    13981 ggcggatctt ggcgtcgaat tgctcggcca ggtattgcct ggcgcaggcg atctcggcct
    14041 ttagtgcagc gcgtggacag gggaggttag ccaggtgtcc cgagcggaat tcccgggaga
    14101 agatcgcaaa ggttgccacc agggccaagg tcgctaccag ataccgacgc tccatactgc
    14161 ctcctcgctg ctcataagac gtgagaacgt ggcgatggtt agtgaactct tttacatttc
    14221 taaggccaag ttctgcaatg cctgcagaag taactacacg ttggttgcca aacgccggcc
    14281 gttcgtaagt tgatgtgccg cggatggata ggtctcattt gtgaatgagg tgtgaatagc
    14341 aggccgtcca aaagcagaag gggttgtccg atggcggaca gtcgaggctt tcgggcacgt
    14401 cgagaccgag cgctgcacgt cattttggtg ctaggctgtt gcgatcatga ctgcggaaga
    14461 agtcaaagaa ctgttgcaac tgacgccgct agagttggaa ggcgggttct ttcgcgaaac
    14521 ctatcgctcg cgctggcagg tgtgggcgga gtatctgccg gaaggcacgc acggttcgcg
    14581 ttccatcggc acggcaatct attacatgat cacgcccgaa tcgttttcga cactgcatcg
    14641 gctgccggga accgaggtct tccacttcta tatgggagat cctgtggtca tgctgcaact
    14701 gcatccagat ggcgcgtcgc gcacagtgac gctcggcacc gatttagcca gtgggcagga
    14761 accgcaagtc gtagtgcgag gaaatgtgtg gcaaggctgc aggctcgcgc ccggcggcaa
    14821 gtgggcgctg ctggggacga cgatgtctcc agggtttgat tatgccgact atgtgactgg
    14881 ggatcgcgat gagttgatcg cgcagtatcc cgaggtggcg gagatgattc ggaaatacac
    14941 gcggtagtcg gtgcgatcag tactcagcaa tttacccgca cttccctgtc ccatgacact
    15001 tgcgaccagg cgaggagcaa tttcccggct cggccaactg gccatccgga ccgatgcagg
    15061 tctgggtgtg gaggcaccag aacaggccat cgttggtcgg cgggaccgtc gggtcctggt
    15121 ccacgccgat gaattgctgc ttccacctca gggctgtgca cagggtttcg caactggtat
    15181 tgaagcgttc gtgatcgggc aatagcatca tgcggcgcct ccaaatatgg aactgccaga
    15241 gtcgccgttg gcagcgttga ggatggcgcg cttgaccgcc gccttggtta acgtgatctt
    15301 gtacttgttg cggctgagcg gtttggcttg cgccaccgcg gcgttcgcgg ctgccatcgc
    15361 ggtgtcgggc gtgatcgtct tgcccaccag cgcctgcgcg acttcaggcg agatccatgg
    15421 aataggggcc acgtgtccga gcaccacgcg cgcgcttttg actgcgttgc catccatctc
    15481 gagcgcgaca cttgccatgg cgagcggcca gtcgaaggct gcccgctgcc gcacttcgta
    15541 gctggcggcc ttcaggttct gcggcggagg aggaacgatc aactcgatga ccatctcgtc
    15601 gagcttcaaa tcgtgctcgc gctggccgtc ggcggtaggg atgacgaaga atttctccag
    15661 cggcaactcg cgcactcctg aaccggaagc tttcgctgag tagatgcgaa tcttcgctcc
    15721 ataagcgata agcacgggag cgatggtcga tgggctgacg aaatacgctg gcccgtcatt
    15781 gccgagaatc gcggcgtaac gattgtcgcc ttcaaccacc gtcgacttgc cgttgggcgc
    15841 tttaggcagc aggcccatac cattgcgaaa gtaccagcag cgcgggcgct ggcacatgtt
    15901 gcctcccatg gtcgcaaggt tgcgaatctg gggactcgcg gcctcgatgg ccgcttccgc
    15961 caggacagga tagtgctggc gcacggttgc gtcgtcagca aactcggcaa tcgtggtcaa
    16021 cgctccgata cgaaggccgt tgccgggctg gtaggtgatg ccgcgcagtc cgtcgatgtg
    16081 ttttacattg accagtcgct tgggggtgac gacatcgtcc ttcatcagcg ccagcagatc
    16141 ggtgcctccg gcgaggatgg cgccttgatc gccgattaac gcggcgacct gttctttttt
    16201 gctagcgctg gcgtattcaa acgcttgcat gggcgcctcc ttgctcgagg gcttccagca
    16261 cgcggtcggg agtgagcggc aggaagggca cgcgcactcc gatggcgttg gccacggcat
    16321 tcgagatcgc cgcgccgggt gagacgactg cgggttcggc caggccaatc acgccgcgtt
    16381 cgtcgtagcc tttgccggtc atcatgtgga ctttgagttc gccaatgtcg ctgaggcccg
    16441 cgaggcggta gaactccagg ttcggattca ggacccgccc gctcacggga tcgaggacct
    16501 tctcttcgta gagcgcgtag ctgatgccca tgatgaggcc gccgtaaacc tggctcatgg
    16561 cggtcttcat gtccacgatc aagccgcaat cctgcaccgc gaccattttg ttgatgcgca
    16621 cgataccggt ctcgacgtcc acggagacgt ccgccatctg cacgccgccg acgccgctgt
    16681 tggtgaggtc aggcggcttg gccttgtcgg gattactgcc gtgcacggtc aacggaaccg
    16741 cgccaatctt ggcgcaggct tgcttccacg gcaggctgcg gctgggatcg gctttcacac
    16801 gcaccgtgcc atccgcggct tccaattcgt cgggctgagc tttgagcgcc ggagcgactt
    16861 tggcgcagag ttggtcgcgc gcgtcgaccg ccgctcgacg ggtcgatgct gtaacgccac
    16921 cggacgttgt actgccgcca ctgccgccgg actggggata gcgattgttg ccatagagca
    16981 gattgacgct ctccatcggg atgccaagcg tgtcggcggc gacgatggtg atgatggtgc
    17041 gcgcgccggt gcccaggtcc tgcgtgccca tcttgatttc gactgagcca tcgggatgga
    17101 tggtgaggtc gcaattgctg ttgtggccgc gacctcccca ggtgtgcatg gagagaccga
    17161 gaccgcgctt gattgggcct ggagttttgt cgccgcgctg atgccagttc ttcttccatc
    17221 ccatgaggtc gtcggcgatt gcgaactctt cgcggtaggt ctgggcgcgc gggcctgcaa
    17281 tgtcgagatt tcggctgatg aactccagtg ggtcgaggtt catcttggcg gccaggtctt
    17341 cgattgcacc catggtgatg accgccgctt gcggatgatt gggcgcacgc caggcgcggg
    17401 cacttccctg attggtggag atggccgtgt tctgcttgcg ctggtgggga atttcgaaga
    17461 tgtatggcaa gggaggagtt ccgccgccgc ccacgccgcc ggtcccccag gaacgcgaat
    17521 cccacgcgac gatcttgccg tctttggcgg cggcaatctt cacgcgggcg taggccgagg
    17581 gacgcatgcc tgccacctcg aactcggcac ggcggtcgag catgttcttc accggcttgc
    17641 cggccgattt cctggagagg ttggcggtat ttacatccca gcggtccacg gaaaacttgc
    17701 tgccaaaacc accgcccacg ttctgctgca ggacattgat attcccggcc ggaacaccta
    17761 acggctcagc caactgcgcc gcaatgccgg aaacgttttg cgtcgatacg tggactacga
    17821 cttctttttc ggcgggccat tccatggcag acccgtgggt ctcgaggcag acgtgcacaa
    17881 tgcagggcac gccgtaaacg ccctcgacta ctgcggccgc cgaggcgaag gccttgtcgg
    17941 gagtacccgt ggtttgcaag cccgttttct tgtagggcga tttcggcttg gcttcggcgg
    18001 gaacgaattt cgctttgcgc agggcggcga tcacggccgg ctcgacgccc atgcccttca
    18061 tcgactcgta atctttttcg tcgggcttga agctgatgcc tcgcttctga atcgcagcga
    18121 tgacttcctc gtccggaacc tggttctctt ccatgtcctg aaaatcgtcg ttgctgagcg
    18181 ggccggagtc agtgctgatg ttcttcggag gctcggcatc ggagacgaaa tgcggcaaca
    18241 cctcgtactc gaccttgatg gcgcgaaggg cgtcttcgac tgtgccttca tcgaccgcgg
    18301 ctacgcccac gatttcatcg cctgcccaga aaatttccgt gccgggcttc tggatgatga
    18361 ccacggattt cacgccgggc atcttttcgg cggccgatgt atcgatggac ttgattttgg
    18421 cgtgggcgta ggggcagcgc aggatttttc ccgccagcag tccctgcggg ttgtagtcgt
    18481 aggtgtattt cgctttcccc gagaccttgg aggggccgtc gacgcgatca attttcttac
    18541 caaggagggg acgggttgcg ggatcgggcc aactgtactg gctcatgact gcttacctcc
    18601 cgcatgggcg agggctaccg cgcggattcc gtcgtaggtc ccgcagcgac aatagttgcc
    18661 gctcaggcca tggcggatgt cctcggcgct gggcttggga ttctgctgaa gaaagtgggt
    18721 ggtggcgacg acgaatccgg gagtgcagaa gccgcattgc tgcgcgtcgt gcgcgacgaa
    18781 ggcctgcatc accgggctca gcgcatcacc ttcggccatt gattcgatgg tggtgatctt
    18841 cgtcccctgg gcatcgatcg ccagcactga acaggcatac actggttttc catccagcag
    18901 aacggtgcag gcgccacagg caccgcgatc gcagacccgc ttggcgccgg tgaggtcgaa
    18961 ctgctcacgc aacgcgtcga gtagcgtcac gcgcggttcg aggtcggcgg taagtttctt
    19021 gccattgacg ttgagcgcga ccagtacttt accgggacca tgaacagtaa cgtcttcccc
    19081 ggctgcttcg accacttttg gtcccagaac caacggaacc gcggcggtga cggccgagat
    19141 cttcatgaag tcgcgtcgcg aaactcccga agtcgaatca ggctggcgcg cgtcgtcgtc
    19201 tttcatcttg cctccacaaa tgagtacagc ggagcatttc tgttcataga cgttgatggg
    19261 aatagttggt tgcagaatcg gtaattatgt tggcaatttc cgtcgtgatg tggatcgacg
    19321 agaccgaccg atcagcgcga gtataactct ggcggtggat cacgggatag cggcggcgcg
    19381 actttaaaca gattgatcga aagcaaagag agcgcaacaa gaacgggcga cgacttctgc
    19441 gagccgccac cccgttttga aaccagcttg tccggcttgc ttacttcaaa gtgtgaacgt
    19501 acttcacaac atcggtgatc tgggcgtctg tcagtttacc tttgaacgcc ggcattttgc
    19561 ctttgccgtt ggtgatgaga gcgctcatgt cggcatcgga tattttctgg acatccgggc
    19621 tgcccagcgg cttgagcccc atgctcttgc ccatgcctgt gctggcgctg ccgtcagctc
    19681 cgtggcacat ggcgcacttt gccttgaagg tgtccgcgcc ggtgccctgg gcggtggcga
    19741 tcatcggaac cgccagcgct aaagccagcc ctactagtgc gatcttcttt accaaactca
    19801 ttcttatctc cttcgcccca actacggtgc gggtgttcca tgaagaaata aataccgtcc
    19861 gagacggcgt ccctactgta actctccggg ccgcacttgg ctacaccgga tcctcctgac
    19921 ttacgaggga aaaaacacgc agggcgggtg atatgccgca agtttggtct gtcgagtccc
    19981 cgccaatctc tattaccaca gggttaagtg cattgttttc agcgttctta tgcatattgg
    20041 acgaaccgtc gagtttggca tccgtagtgc ccttaccttc tgtattccta tttgcgggag
    20101 agtgtcaccg tggaaccgac attggcagta atcgggctga actttcggac ttcgccggtg
    20161 gctatccggg agcggttctg gatcgccgaa ccccgccgct atgagacatt gcatcaattg
    20221 gtgcggtcag agggcatcga agaggtggcc gtgctggcca cctgcaatcg caccgagttc
    20281 atcgtgtggg ccaacgacgt cccggaggcg tccaactcgg tattacgatt cctgacgcag
    20341 gaatatcaac tgaaattgtg cgagtggtcg cacttctatc gcttgatgga cgacgccgct
    20401 ctcacccaca ttttccaggt ggcagccgga ttggactcga tggtgctggg cgagcccgaa
    20461 atcgcggcac acctgcggga agcattgcaa caagcacaaa aatccggaac ttcaggccgc
    20521 ttcctcgatg gcattatcca gaaagctttg gcggtatcgg cgcgtgtccg cagcgaaact
    20581 gccatcgccg attcgaactt gacggttccc ttcgcttcgg tcgagctttc gattgatgtg
    20641 ctgggagatc tatcgggatg tgaagtcctg ctggtgggcg caggaaaggt gagcgagctg
    20701 gccgcaaaat gcctgaggag cgccggcgcc actcgcatta aggtgatgaa ccggacccag
    20761 gcgaacgcgg aagaactcgc gctcaaactc aacgccaaag cggtatcgtg cgaagatcgg
    20821 ctatcgcacc tgaaatccgc cgacatcgta gtgtgttcca catcgtgtcc gcacagcatc
    20881 atcagcctcg aagacgcgca gcacattgcg cgcgcgcggc aacacaaacc gatggtgatg
    20941 atcgatatgg ccgtgccgcg cgacattgac cccagggtgg gagaggtcga cggcatccac
    21001 ctgtttgata tggatgacct cgaacgggtg gtgaagaggg acatccgcga gcggcgtgcc
    21061 gccgccgatg aggcgaacga gatcgtggtc cacgaagtgc tgggttttcg ccgcaagctc
    21121 atggtggaac gggtaattcc gacggttgtg gcgttgcgcc ggcggttgga ggaactctgc
    21181 cacgaggaat tggagttcgt tcgtaaggag tatgggccgt tcaccgacga tcaggaccag
    21241 atactgacca cgctcgcggc acacattacg cagcggctgg caggctcgct ggcccgcgaa
    21301 ctacgagaac ttccggaccg caccgggcag gaaatgctga ccgtggcggt gcatcgcctc
    21361 ttccagctgg aaccgtccgt catgggggaa gccggaaaag agaactgaca gtaaagcatc
    21421 actttcgaag gagcaggaaa tgcctgaatc tgcgcaagac aagacaaaca cgactctcgg
    21481 ctttaccagc gtgcaagcct acacgctggc ggtaatcacg ttagtgattg gaattgcagt
    21541 tgggtacttt gcccgcggct cagcgccagc ggcggttgcc cccgaagctg cacaaaccgc
    21601 agccgcgacg gcacctgcgg gaatgggtgg ccgggccggc atgggtgccg ggcaattgcc
    21661 gggcatcggc agccagcaac aggggggaag ttcgcctgag atgctggcgc aagcggccca
    21721 gccactgctc accaagctgc aagccactcc caaggacttc gaaacgctgc ggcaactggg
    21781 aaacttgtac tacgacggcc aggcgtttcc gcaggccatc gagtactaca acaaagcgct
    21841 cacgattgac cccaagaatc ccgatgtgct gaccgacgtg gggaccgcgt attggtacag
    21901 cggagacgcg gacaaggcgc tggcggaatt cggtaagtcg ctagccgtcc gaccgaacca
    21961 tcccgggacg atgttcaacg aggggatcgt gatgtggcag ggcaagaaag atcccaaggg
    22021 cgcagtggtg gtgtgggagc agttgctgaa gaccaatccc aactacgagc agaaggaaca
    22081 agttcagatg ctgattgagc gcgcgaaaat gcacggagcc ggcgctacga agggctaagt
    22141 ttcgtgaagc acggagcggt tgctcctgcg ttcgcattcg ctcaccccgg
//
LOCUS       DASRKK010000617         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_935045, whole genome shotgun sequence.
ACCESSION   DASRKK010000617 DASRKK010000000
VERSION     DASRKK010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935045"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1584)
                     /gene="ltrA"
                     /locus_tag="VFC15_14970"
     CDS             complement(229..1584)
                     /gene="ltrA"
                     /locus_tag="VFC15_14970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM11506.1"
                     /translation="MGEPTTENWELAGAESVGPLPEKLSLLRQKLHRKAKQEPRFRFY
                     ALYDRIYRRDTLEAAWKRVRANKGAPGVDGQSIEEVEKSEAGVEGFLDEIQESLRAKT
                     YQAQPVRRVYIEKDNGKLRPLGIPTVRDRVIQMAALLILEPIFEADFEDCSYGFRPDR
                     SAHQALQEIRGHLQRGLQAVYDADLKGYFDSIPQDKLMACLRMRVVDRSVLKLIRMWL
                     ETPVMEPGGEGGKPAKWSRSEKGTPQGGVISPLLANLYLHWFDKEFHRASGPAQWAGA
                     KLVRYADDFVVLSRYPGKRLYGWIESKLEQWLKLEINRDKTRVIDLGQEKAKLDFLGY
                     TFCWYRDRYGRGNRYLHVGPSKKALQRERNKLTEMTDSHQCHTPIPQLIEGLNRHLRG
                     WGNYFGFGYPREAFREINWHVGHRLFRHLNRRSQRAFRLPEKTTYYQHFRRLGLKPLE
                     I"
BASE COUNT          496 a          831 c          638 g          620 t
ORIGIN      
        1 gtgttacgct gacatgcaac ggcgtgtccg cgaatagcgt gaccctggta tttcaataac
       61 tcaggagatt gattgtggta ccaggttgct gaaaaagcgc attttttagc cggtagagta
      121 ggacggggag caacggattg atccgcgact tcccgtcccc tcgtcgaacc ggacgtgcgg
      181 atttcccgca tccggctctc ctgaaaacct tggtcacagg ctcacgcatt agatctccag
      241 gggtttgagc ccaagacgcc ggaagtgttg gtagtacgta gttttctccg gcagtcggaa
      301 ggcccgctgg cttcgccggt tcaggtgcct gaacagacgg tggcccacat gccagttgat
      361 ctctcgaaac gcctcccgcg gatacccaaa cccgaagtag ttcccccagc ctctcaagtg
      421 ccgattcagc ccctcaatta gttgcggtat cggcgtatga cattgatgac tgtccgtcat
      481 ctccgtcagc ttgttccgct cccgttgcag tgccttcttc gacggtccca catgcagata
      541 ccggttgccg cgcccgtacc ggtcgcgata ccagcagaat gtgtaaccca gaaagtccag
      601 cttcgccttc tcctggccca gatcgatcac gcgcgtcttg tcccgattga tctccagctt
      661 caaccactgt tccagtttcg attcgatcca gccgtacaga cgtttgccgg ggtagcgcga
      721 cagcacgaca aaatcatcgg cataccgcac cagtttggct cccgcccact gcgctgggcc
      781 actggctcga tggaactctt tatcgaacca gtgcaaatac aggttcgcca gcaagggcga
      841 tatcactcct ccctgcggcg ttcccttctc cgagcggctc cacttcgccg gcttgcctcc
      901 ctccccgccc ggttccatga ctggtgtttc cagccacatc cggatcagtt tcaggacgct
      961 ccggtccacc actcgcatcc gcagacacgc catcagcttg tcttgcggta tcgaatcgaa
     1021 ataccctttc aagtccgcgt cgtacaccgc ttgcaggcct cgctgcaaat gtcctcggat
     1081 ctcctgcagc gcttgatgcg ccgagcggtc cggccggaac ccataggaac aatcttcaaa
     1141 gtcggcttcg aagatcggtt ccaagatcaa cagggccgcc atttggatca cccggtctcg
     1201 caccgtcggt atccccaagg gtcgcaactt gccgttgtcc ttctcgatgt acacgcgcct
     1261 cactggctgc gcttggtagg tcttggcacg caacgattcc tgaatctcat ccaggaatcc
     1321 ttccacgccc gcttccgact tctcgacctc ttcgatcgat tggccatcca caccaggtgc
     1381 tcccttgttg gctcgcaccc ttttccatgc cgcctccagc gtatctcgcc gatagatccg
     1441 atcatataat gcataaaatc ggaatctcgg ctcctgcttc gccttccgat gtagtttctg
     1501 tctcagcaaa gagagtttct ccggcagcgg accaacgctt tccgcgcctg ccaactccca
     1561 gttttccgta gtgggttctc ccaagcggct ctcctttcct cttacctaaa cttgtttcca
     1621 gtgaggtccc ttcgctccac ggtcgttacc cgcttcttcg ctactatggg cctctccgat
     1681 tcccggttgt gctccgcctt cggttatgta ttcccgctgg cgggcgtgtg gcttgcgcca
     1741 caccccacat ccgggcctcc caggttcctc tgccgatctt tccctgcgcg ctgccctcaa
     1801 ccaccccgag tgctccggtg gccctacgtc cgttggcgct tcaccggtaa caggtttcac
     1861 caaatcaggc tggctaacca agcactattt gcgtaacgag gccgaatcag gtttgcttgc
     1921 gctgcggctc gcaggttcgc ttttcgaggc ttcgccgcac ggattgctcc gcactgcgct
     1981 cgatagctac atgtcgaacg gataattcac atggtgagtt cctttcatct cactagcggg
     2041 agtttcctaa agcatcattc ccaaattctc ccaagacaaa gacgacaaac gacttagcac
     2101 cagtcggtct gtatatacgg aattgcgagg ccttcctcat cgtaggcctc tcagattttg
     2161 agattgacac gccatcgcgg ttgctgtata catgtgatca atggctctta atggctctat
     2221 gcccaggcag gtgaagcagt tagtgaacct gggacgacgg cttaaacgcg tggtatatac
     2281 acccgcttcg ccgtaaacgg tttatcaaca gacacttgac cctgtttctg gctgtcgtct
     2341 taggaaactc ccgctagatc ggccaggctt gtcctggcgc acccgattca cgccgataaa
     2401 accaagtctt tttcttacct gcgtgaatcg gcgttcatcg gcggccaaca ccaaagacgg
     2461 ggaggcacgg gggtgtggtt acggctgtaa cggcgccttg gcggactacc gaacccgata
     2521 gacgaaggcg tcttttagga aacggccgcc gatcaattga ccgccgagac gcatcccctc
     2581 aacgc
//
LOCUS       DASRKK010000618         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_55842, whole genome shotgun sequence.
ACCESSION   DASRKK010000618 DASRKK010000000
VERSION     DASRKK010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55842"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFC15_14975"
     CDS             <1..1066
                     /locus_tag="VFC15_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZM11507.1"
                     /translation="IPLSDRSFYFEQLRRSEFDFNSQSARPYFPYDRVQQGILDVASK
                     LFHVTFQPAKDAVVWDPSVAAFDVFDGPKQLGRIYLDMHPRPGKDQWFSSNPLLDGKR
                     GKQLPEAVLVCNFSGGKPGEPGLMEYGEVTTFFHEFGHLMHWIFQGQQQWAGYSGNLE
                     ADFVEAPSQMLEEWMHDPKVLATFALNYETNQPIPAELVQRANRADAFGRGLWARFQL
                     VYTSVSFDLHNVPPDPAQIEKSFPENIKRFVPFGFVEGDHQIASFTHLTGYSSAYYTY
                     LWDKVIAEDFFSKFDRNDLLAPEVALRYRSTVLEKTGSMPANDLVKSFLGRPQALDAF
                     KGWMNQEFQGLPAAGQPSGN"
     gene            1301..1858
                     /locus_tag="VFC15_14980"
     CDS             1301..1858
                     /locus_tag="VFC15_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11508.1"
                     /translation="MVSLSALWIPIVLSAVIVFVASSIIHMVIPYHRGDYQKVPDEDK
                     VRAALRAAGLKRGLYMFPHGTHKDMKLPAMMEKYQQGPVGILTMFDNGVPVMPKFLGQ
                     WFVFCLIISFFVAYLAAHTLAPGTYYLEVFRIVGTAAFLGYGLGHLSNGIWKGQPWSV
                     TIKEVIDGLIYGLLTAGTFGWLWPR"
     gene            complement(1953..3104)
                     /locus_tag="VFC15_14985"
     CDS             complement(1953..3104)
                     /locus_tag="VFC15_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM11509.1"
                     /translation="MRRILLCFLLCCCACLRAEEKNIDTKILVPVVADQGGKPVLDLQ
                     ATNFSVDGPKGIQLSSVQMVPAEAMNDPQHTFPVFILYDAYFFTAPVQAMVAADLLQF
                     LTDLSQHYSPVTLVVNTPMGLKLVYDFGTDPKILGEALAALDTGAKVSDPKVKDQLEK
                     LKNLKNFVPAPAAVEDLTIVQFGGLEHMAQVLGRSPNRKALVWMTWNYRLGIGDHTVN
                     WTGSTASQDKNLTMNTGTRAEVYANNLADTRYQNLPPLYEKAIAAANGAHVSVYPLQQ
                     YDRRDTNLSFNNPSDQVTTEGLRQVAACTGGTSIKDFKQTSVAQAVAQVRAGFGPYYM
                     LTLTAGDIKKTDWIPLKVKVDRPAVTIRSAPGFLALSPSAAKSAGIVAK"
     gene            complement(3348..>3858)
                     /locus_tag="VFC15_14990"
     CDS             complement(3348..>3858)
                     /locus_tag="VFC15_14990"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HZM11510.1"
                     /translation="DYWTGQRVELKSQDDKEMKGKLHLNPKLDEIPVFVRAGSIIPRQ
                     PLVQNTSQKPNGPLELRVYPGPKCLDYVYTDDGNSFGYKRGEYYRGQLSCESSGNSVK
                     VTIAGGEGKYTPWWTSYNVVVVDAPKAPASVMVGGKTASDFHYDVKGKSVAVTVPFAQ
                     STNEVVIQY"
BASE COUNT          730 a         1217 c         1032 g          879 t
ORIGIN      
        1 gatcccgctc tccgaccgca gcttttattt cgagcagctt cgccgcagcg agttcgattt
       61 caactcacag tccgcgcgcc cttacttccc ctatgatcgc gtgcagcagg gcattctcga
      121 cgtcgcctcc aagcttttcc acgtgacctt ccagcccgcc aaagacgctg tggtctggga
      181 cccatccgtc gccgcgtttg atgtctttga cggcccgaaa caactgggac gcatctacct
      241 cgacatgcat ccgcgtcccg gcaaggacca gtggttcagc tcaaacccat tactcgatgg
      301 caagcgtggc aagcagttgc ctgaagccgt gctggtctgc aatttctccg gcggcaagcc
      361 cggcgagccc ggactgatgg agtacggcga agtcactacc ttcttccacg aattcggcca
      421 ccttatgcac tggatattcc agggtcagca gcagtgggcg ggatacagcg gcaacctcga
      481 ggcggatttc gtcgaggccc cttcgcagat gctggaagaa tggatgcacg accccaaggt
      541 gctggccacc ttcgcgctga actacgaaac gaatcagccc atccccgcgg agttggtgca
      601 acgcgccaac cgggctgacg cttttggacg cggcctctgg gcgcgcttcc aactggtgta
      661 caccagcgtg tcgttcgacc tgcacaacgt gccgcccgat cccgcgcaga tcgaaaagtc
      721 attccccgaa aacatcaagc gctttgtgcc cttcgggttt gtcgaaggcg accatcagat
      781 cgcctcgttc actcatctga cgggctattc gtccgcctac tacacctact tgtgggacaa
      841 ggtgattgcc gaggacttct tcagcaagtt cgatcgcaac gatctgctgg ctccggaagt
      901 cgcgctgcgc tatcgctcaa cggtgctgga gaagactggc tcgatgccgg ccaatgacct
      961 ggtgaaaagt ttcctgggac gtccgcaggc gttggacgcc ttcaaaggtt ggatgaacca
     1021 ggaattccag ggcctgccgg ccgctggcca gccctcagga aattagcttc tacgttgcgg
     1081 atattcgcaa cttgcaactg aatcacgtac cccgctcaag ccgggtttgc tggagtgggt
     1141 ctttatccgg acgcaatagc gtgtcccgcc cacggtgctt gagcgggttt ttctggactc
     1201 gaaactgact ttagcagctc ccgcttgacc gcgcaccgct ctgggatagc ctcgcgggga
     1261 aaatttccgc attcgccttt gcgccctaag gaggctgcca atggtctcac tctccgccct
     1321 ctggatcccc atcgtcttgt cagctgtcat cgtgttcgtg gccagctcca ttattcacat
     1381 ggtgatcccg taccatcgcg gcgactacca gaaggtcccc gacgaagaca aggtgcgcgc
     1441 tgcgctgcgc gccgccggcc taaagcgcgg tctctatatg ttcccgcatg gcacgcacaa
     1501 agacatgaag ttacccgcca tgatggagaa ataccagcag ggcccggttg gcatcctgac
     1561 catgtttgat aacggggttc cggtgatgcc gaaatttctc ggccagtggt tcgtcttttg
     1621 cctgataatc agcttcttcg tcgcctacct cgcggcgcat accctcgcgc ccggtaccta
     1681 ctatctggaa gtctttcgca tcgtcggcac cgcggcgttc ctcggctatg gtctgggaca
     1741 tctcagcaac ggcatctgga agggccagcc ctggagtgtc accatcaaag aagtaatcga
     1801 cggacttatc tacggcctgc tgactgcggg aactttcgga tggctctggc cccgctagct
     1861 cgtttttcac gactgcagac cgagtactcc gttcaacatt ccggactagg gcggagtact
     1921 tccccctagc atcatggctg aattcttcac cctcacttgg ccacgatgcc cgcactcttc
     1981 gccgcgctgg gagacaatgc cagaaatcct ggcgcgctgc gaatggttac cgccggccga
     2041 tcgactttta ccttgagcgg aatccagtct gtcttcttga tatcgcccgc cgtgagcgtg
     2101 agcatgtagt agggtccgaa cccggcgcgg acctgcgcga ccgcctgagc gacgctggtc
     2161 tgcttgaagt ctttgatgct ggttcccccg gtgcatgctg ccacctgtcg caatccctcg
     2221 gtggtgacct ggtcgctggg attgttaaac gacagattgg tatcgcgccg gtcgtactgt
     2281 tgcaacggat acacactcac gtgtgccccg ttcgcagcgg ctatcgcttt ctcgtacaac
     2341 ggcggcaagt tctggtagcg cgtgtcggcc aggttgttgg cgtacacctc ggcgcgcgtc
     2401 cccgtattca tcgtaaggtt cttgtcctgc gatgcagtgc tgcccgtcca attcactgtg
     2461 tgatctccga taccaagccg gtagttccag gtcatccaca ctagtgcctt gcggttgggc
     2521 gaacgcccca gcacttgcgc catgtgttcc aatccgccga attggacgat ggtgagatct
     2581 tccactgcgg ctggcgccgg cacgaaattc ttcaagtttt tcagcttctc caattgatct
     2641 ttcactttcg ggtcagacac cttggcgccg gtgtccaggg ccgccagcgc ctcccctagt
     2701 atcttcggat cggtgccgaa gtcatacacc agcttcaatc ccatgggcgt gttcaccacc
     2761 agcgtgaccg ggctatagtg ctgcgagagg tcggtcagga attgcagcag gtcggcggca
     2821 accatggcct gcaccggagc ggtgaagaag tacgcgtcat ataggatgaa caccggaaac
     2881 gtatgctgcg gatcgttcat cgcctcggcg ggaaccatct gcacgctgga gagttgtatc
     2941 cccttcgggc cgtccacgct gaaattcgtt gcctgaagat cgagtaccgg cttgccccct
     3001 tgatcggcca ccaccggcac caaaatcttc gtatcgatgt ttttttcttc ggcccgcagg
     3061 cacgcacagc aacacagaag gaagcacaac agaatgcgac gcatgaacga cacctcgcgc
     3121 aagaaattaa aaacgtgaaa ttatagagcc gttcatctct cctgcctgtg acccgtgtcg
     3181 caccccattg gtcatgtgaa atagctccgc tatgcgcagg ctgcccaggt tcgcgccggt
     3241 ctcttgccgc tttcccggct acggttcccg cacgacgccg gtctcatccc gattttttcc
     3301 atgacagcgc atgggccgcc gtctcgtact gccttgtccg cttcctctca atactgaatc
     3361 acaacttcgt tcgtggactg tgcgaaaggc accgtgacgg ccacactctt ccccttcaca
     3421 tcgtagtgaa agtcactggc cgttttaccg ccaaccatga ccgaggctgg cgctttcggc
     3481 gcgtccacaa ctaccacatt gtaggacgtc caccacggag tatatttccc ttcgccgcca
     3541 gcgatcgtca ccttcaccga gttgcccgat gactcgcaac tgagctgacc ccggtagtac
     3601 tcaccacgct tgtagccaaa gctgttccca tcatcggtgt agacgtagtc caggcacttg
     3661 ggtccgggat acaccctcag ctccagcggc ccattcggtt tctggctagt gttctgtact
     3721 aacggctggc gcgggatgat cgatccagcc cgcacgaaca ccggaatctc atccagcttg
     3781 ggattgagat gcagcttgcc cttcatctct ttgtcgtcct ggcttttcag ttcgacccgc
     3841 tgccctgtcc agtagtcg
//
LOCUS       DASRKK010000619         6596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_544102, whole genome shotgun sequence.
ACCESSION   DASRKK010000619 DASRKK010000000
VERSION     DASRKK010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544102"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFC15_14995"
     CDS             complement(<1..516)
                     /locus_tag="VFC15_14995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM11511.1"
                     /translation="MDRFAILFDVNFIRQTVSTSDLTVGLDYTILDFGRSSRIESAAA
                     QLLATDFGFNDAHRRVIYQVEEAYYRVLSTASQQLGAEANLANARAVQQAAQDRLDHG
                     LATGPDVLEAQSATTQAQYDLQSAIGAQEIARGDLAAALGTAASVAIQIAPVTSLTTP
                     DSMEDSVEQLIN"
     gene            complement(997..1473)
                     /locus_tag="VFC15_15000"
     CDS             complement(997..1473)
                     /locus_tag="VFC15_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZM11512.1"
                     /translation="MNERTGDLLILGIGNLLMGDEGVGVHVVRHLEKLGVPEGVELLD
                     GGTGSFLLLEPMNRARRIILIDATLDSQPPGTVRRLTPRFSTDYPRTLTAHDIGLKDL
                     LDTLYLLDHPVDIVLFAVSIASLQEISLDLCPEIAERVPEIAQLVLNEVMSDRLPV"
     gene            complement(1466..2152)
                     /gene="cybH"
                     /locus_tag="VFC15_15005"
     CDS             complement(1466..2152)
                     /gene="cybH"
                     /locus_tag="VFC15_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HZM11513.1"
                     /translation="MAHASVATYERVYVWELPVRFYHWVNALCVVVLIVTGFIIGHPV
                     SILYASEAYQQYWFGTVRFAHFLAAFVFFFMFLVRIYWGFVGNAYARWTNFIPYKQEQ
                     IEEIGDIVKVDILQTKIRGDISIGHNALAGFIYFLSFLAFLFQSATGFALYSSMSRSA
                     LPHMFAWIIPLMGGDFRVRQWHHAMMWFFVLFAIVHVYLVFYHDYIEGRGTTSSMVGG
                     WKFVRRSGHE"
     gene            complement(2163..3878)
                     /locus_tag="VFC15_15010"
     CDS             complement(2163..3878)
                     /locus_tag="VFC15_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HZM11514.1"
                     /translation="MARLVVDPITRIEGHLRIEAVVENGTITDAYSSGTMVRGFEKIL
                     KGRDPRDAWAFTERACGVCTTVHALASVRTVEDSLSITVPRNAELIRNIMFCTQYVQD
                     HVVHFYHLHALDWVDVVSALKADPQKTSEIAVSISPWPKSSPKYFAGVQQKLKAFVES
                     GQLGIFANGYWGNPSYKLPAEVNLLAVTHYLEALEWQREIVKIHTIFGGKNPHPNYLV
                     GGVPCSINPDEVGAINMDRLNYVSSLISGAIAFVEQVYLPDLMAIAGFYKDWAAIGGG
                     LKNYLAYGDLPTRGYNQPDYFKFARGAILDRNLNEVYPVNGKDVEEIKEYISHSWYSY
                     SAGDAAGLHPFAGETEFNYTGPMPPYEQLNVEGKYSWLKTPRWKDHAMEVGPLARLLV
                     SYAKGSTEVKDVVNEALGRLGVPVTALFSTLGRTAARGLESRLVVHWLKGFFDDLMAQ
                     VKVGELSTFTRDKWEPSTWPATAEGVGLTEAPRGALAHWIRIKDGKIDNYQLVVPSTW
                     NASPRDPKGQRSAYEEALIGTHLANPEQPLEILRTIHSFDPCIACAVHLYDPHGKHIN
                     RVQFY"
     gene            complement(3938..5050)
                     /locus_tag="VFC15_15015"
     CDS             complement(3938..5050)
                     /locus_tag="VFC15_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HZM11515.1"
                     /translation="MKASSGSIWDVMRSKGYSRRDFMQFCGYAAMVAGISKANLGAVA
                     QAFEKKARPPVVWLHFQECTCCSESFIRSSHPIVADVLLDLISLNYTETLQAAAGFQA
                     EKCLTDTIKNDAGKYILLVEGSVPVKGDGVYCTIAGKTAESILQDAAKDAAAVIAWGS
                     CASNGCIQSAKPNPTGATPIHKLINKPVINVPGCPPIADVMTGVVTHVLVLGRGPELD
                     SLGRPREFYSRRVHDTCYRRAYYDAGLFVESWDDDKARKGYCLYKMGCRGPVTYNACS
                     VTKWNGGVSYPIQSGHGCIGCSEAGFWDNGPFYQHLASFPGFGIESTADTIGATLGVG
                     AVVGVAAHAITTNIRKRKLIEQERGGDETVTQRDET"
     gene            5635..6177
                     /locus_tag="VFC15_15020"
     CDS             5635..6177
                     /locus_tag="VFC15_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HZM11516.1"
                     /translation="MILIDAERRSDWLVKAIDRRLREEFAKLRVEINEDKSRMVDLTK
                     GESFTFLGFEYRRVLSLQRKWRPYYAPKLKKRIALFEKLREIFRQHVSWPVKNVIEKI
                     NPILRGWVNYFRVGHSSLCFGVVKRWVEKKVRQHLMRARGRKGFGWKRWSSEWLYDRL
                     GLFNDYQLRRWSSAKVAPAQ"
BASE COUNT         1392 a         1837 c         1991 g         1376 t
ORIGIN      
        1 gttgatgagc tgctccaccg agtcctccat gctgtccggc gtggtgaggg aagtgacggg
       61 tgcgatctgg atggcgaccg acgccgcagt gccaagtgcg gcggccaggt ctccgcgagc
      121 gatttcctgc gccccgatgg ccgattgcag atcgtactgc gcctgtgtcg tggcgctctg
      181 ggcttccagc acatcgggac ctgtcgcgag gccgtggtcg aggcgatctt gcgcggcctg
      241 ctgtacggct cgcgcgttgg ccaggttggc ttcggcgccc agctgctgcg aggcggtgct
      301 gagcacgcga tagtacgctt cttccacctg ataaatgacg cggcgatgcg cgtcgttgaa
      361 gccgaaatcc gtagcgagca gttgcgcggc agcggactcg atgcgcgagg atcggccgaa
      421 gtcgaggatg gtgtagtcaa gaccgaccgt cagatcggag gtggaaacgg tctggcggat
      481 gaagttgacg tcgaaaagga tggcgaagcg atccacctgc ccgtcggcaa tcgccccgat
      541 ttctcgtagg ccatcgccat ttctccgctc atgcttaggc gatctctcac caactgacac
      601 gacgagtggg ccgacgatgt gtcttgggag tcatactccg cgagcaacgg cccctcgtcg
      661 tcctgctttt gggaagagca aggaaaggag cacggcgccg agcaagaccg aggtgatcca
      721 ggcagcgtag gctgatcgat cggctagcag aaacgcagac ttcgcggcgg cagatatgct
      781 cgtcctggtt gcttgaatgg atccagatca gttgcgatga cctgaagctt caactcaccg
      841 cccgactcct tcctgtctct gatagtcgcg ggtgaaggtt agttcgcatt cgtccacttt
      901 tgagagtcta tcgccgaaag ctcagccaga tccgcgcatg cctcattgca aggcttgcgg
      961 gctttgcgcc aactgccaca agcagatgtc catgaactaa actggcaagc ggtcagacat
     1021 tacctcgttc aaaaccaact gtgcgatttc gggaactcgc tcggcgattt ccggacagag
     1081 gtcgaggcta atctcctgga gcgacgcgat ggagactgca aataggacga tgtcgactgg
     1141 gtggtcgaga agatacaagg tatcaagcag gtctttgagg ccgatgtcat gggccgtaag
     1201 ggtgcgtggg tagtcagtcg agaatctggg cgtcagtcgg cgtaccgtgc cgggcggctg
     1261 gctgtccaaa gtcgcgtcaa tgaggatgat ccggcgcgcc cgattcatgg gttccaaaag
     1321 caggaagctg ccagtaccac cgtcgagaag ttccactccc tccgggactc ccaacttttc
     1381 aaggtgacgc actacgtgga cgcctacgcc ctcatctccc atcagcaagt tgccgatgcc
     1441 gaggatcagc aggtcgccgg tgcgttcatt catggccgct gcggcgcacg aacttccatc
     1501 ccccgaccat ggaggaagta gtgccgcgtc cttcgatgta gtcgtggtag aagaccaggt
     1561 atacgtgcac aatcgcaaac aaaacaaaga accacatcat ggcatgatgc cattggcgca
     1621 cccggaagtc gccgcccatg aggggaataa tccaagcgaa catgtgcggc agtgccgaac
     1681 ggctcatgct ggagtagagc gcgaatcccg tggccgactg aaatagaaac gccaggaaag
     1741 acaggaagta aatgaacccg gccagcgcgt tatggccgat cgaaatgtcc ccacggattt
     1801 tagtctgcag gatgtctact tttacgatgt ccccaatttc ctcaatctgc tcctgcttat
     1861 agggaatgaa attcgtccag cgcgcataag cgttgccgac gaacccccag tagatccgca
     1921 ccaggaacat gaagaagaac acgaatgcgg caaggaaatg ggcgaagcga acagtgccga
     1981 accagtactg ctgatacgcc tcggaggcgt acaggattga gaccggatgg ccgatgatga
     2041 agccagtcac gatcagcacg accacgcata gggcgttcac ccaatggtag aagcggacgg
     2101 gcaactccca cacataaact ctttcgtagg tcgcaacgga tgcgtgagcc atcggcttat
     2161 cctcaataga actggacccg gttaatgtgc ttaccatggg gatcgtaaag gtgcacggcg
     2221 caggcgatac acgggtcaaa ggagtggatc gtccgcagga tttcaagtgg ctgctctgga
     2281 ttggccaggt gcgtcccgat cagggcttcc tcataggcgg agcgttgtcc cttcggatcg
     2341 cggggcgaag cgttccacgt gctgggcacc accagttgat agttgtcgat cttcccgtct
     2401 ttgatcctga tccagtgcgc cagtgcgccg cgcggcgctt ctgttagtcc cacgccctcg
     2461 gcagtcgccg gccaagtcga tggctcccac ttgtcgcgag tgaaggtgga cagttcgcca
     2521 acttttacct gcgccatcag atcgtcgaag aagcctttca gccaatgcac gaccagtcgc
     2581 gattctaatc ctcgcgcggc ggtgcgcccc agggttgaga acagcgcggt gaccgggacc
     2641 ccgaggcggc ccagagcttc gttgaccaca tctttcactt ccgtgctgcc cttggcatag
     2701 gacaccaaca accgtgccag cggcccaacc tccatggcat ggtccttcca gcgcggtgtc
     2761 ttcagccagg aatactttcc ctctacgttg agctgctcgt agggaggcat cggtccggtg
     2821 tagttgaatt cggtctcacc ggcaaaggga tgcagtccgg cagcgtctcc cgcggagtag
     2881 ctataccagg aatgtgagat gtattccttg atctcctcca cgtctttacc atttaccgga
     2941 tacacttcgt tgagattgcg atcgagaatc gcgccccggg caaacttgaa gtagtcaggc
     3001 tggttatatc cgcgcgtggg caggtcgcca taagcaaggt agttctttag accgccgccg
     3061 atcgccgccc aatccttgta gaagccggcg atggccatta agtcgggcag atagacctgc
     3121 tcgacgaagg caatggcgcc gctaatgagg ctgctgacat agttgaggcg gtccatgttg
     3181 atggcaccca cttcatccgg attgatggag cacggcacgc cacccaccag gtagttggga
     3241 tgcggattct tgccgccgaa gatggtgtgg atcttaacga tctcacgctg ccactccagc
     3301 gcttccaggt agtgtgtgac tgcgagtaga tttacttccg ccggcagctt gtacgaggga
     3361 ttgccccaat atccattggc gaagatcccc aactggccgc tctccacgaa cgccttcaac
     3421 ttttgctgga cgccagcaaa atatttgggc gaactcttcg gccagggcga aatagagacg
     3481 gcaatctccg aagtcttctg cggatcggcc ttcagggcgc tgaccacgtc cacccagtcg
     3541 agggcatgca agtgatagaa gtgcacaacg tggtcctgca catattgcgt gcagaacatg
     3601 atgttgcgaa tcagttccgc gttcctgggg accgtgatcg acagcgagtc ttccaccgta
     3661 cgcacgctcg ccagcgcgtg caccgtggtg caaacgccgc aggcacgctc ggtgaaggcc
     3721 cacgcgtcgc gggggtctcg tcctttgagg atcttctcga agccgcgcac catggtaccg
     3781 gaactgtaag cgtcggtgat ggtcccgttc tccaccacag cctcgatacg aaggtgccct
     3841 tcgatgcggg ttatgggatc taccactagt cgtgccatgc gatcacctct tcaaatttcg
     3901 agccgtgctt acccaagtac gctgatggtc cgttcggtta agtctcatcc cgctgggtga
     3961 cagtttcgtc tccaccgcgc tcttgttcga tcagcttgcg tttgcggatg ttggtcgtaa
     4021 tggcatgtgc ggccacgcct accacggcgc cgacgcccag ggtcgcgcca atggtgtcgg
     4081 ccgtgctctc gattccgaat cccgggaaag aagcgagatg ctgatagaac ggcccgttgt
     4141 cccagaagcc ggcctcgctg cagccgatgc aaccgtgacc tgactggatg ggatagctga
     4201 cgccaccgtt ccatttcgtt accgagcagg cgttgtaagt cactggaccg cggcatccca
     4261 tcttgtacag gcaatagccc ttgcgcgcct tgtcgtcgtc ccacgactcg acaaacaagc
     4321 cagcgtcata ataggcgcgg cggtagcagg tatcgtgtac gcgccgcgag tagaactcgc
     4381 gcggacggcc caggctgtcg agctcagggc ctcgacccag caccagaaca tgcgtcacaa
     4441 cgcccgtcat tacgtcggca atcggcgggc agccgggcac gttaatgaca ggtttgttga
     4501 tgagcttgtg gatcggggtc gcgcccgtcg ggttgggttt agccgactgg atgcatccgt
     4561 tggaggcgca actgccccac gcgatgactg ctgccgcatc tttggcggcg tcttgaagaa
     4621 tgctttcggc agtcttgccg gcgattgtgc aatagacacc gtctcccttg actgggaccg
     4681 agccttcgac cagcagaatg tacttgccgg catcgttctt gatggtgtcg gtcaggcact
     4741 tttctgcctg gaagcctgcc gcggcctgca gcgtctcggt gtagttgagt gagatgaggt
     4801 cgagcaagac gtcggcgacg attgggtgcg aagagcggat gaaggactca ctgcagcagg
     4861 tgcactcctg aaaatgtaac cacacgactg gcgggcgcgc cttcttctca aatgcctgag
     4921 caacggcgcc caggttggcc ttcgagattc ccgctaccat cgcggcgtat ccgcagaact
     4981 gcataaaatc gcggcggctg taaccttttg accgcatcac atcccagatc gaaccggaag
     5041 aagctttcat aagccctctc ttctgcaagc ggtgaactgc ggtattcacg ctgtaacggg
     5101 gtcaaagcgt acagccgtac acgttcttca attgcatttt ccctgcgcca gcgagtccca
     5161 cagattctgg agacttcatc acacggtagt acctgctgca gtgactaccg tcacaggtcg
     5221 atgttcgcaa gcgccctcca caccgaagtt ggctgaaagc ggggttccgg aaagaattgg
     5281 attgtgactt cgtctggatg tggcttcccg gaaggaaccc agcttaggac atgcgggtcg
     5341 gtaccaacga gttaccgagt gttagcgaat cagcccgatc caatctgtag gatccaccga
     5401 tccacccgga atgtcagttg gcgaaaacgc aggccttcgc ataaattggc tgttcgttgt
     5461 caagtaaaga cgacttccgg aaggccaatc actgatggtg gagtgtatgg tcggccgaca
     5521 ggaagttatc cagaaaccct agtgctgtat cggatgcgcc ctacagtcgg tttgcaggta
     5581 ggctcgttgc tgcgggcgcg tccgatacag cgctagggtt ttgcggacga cctggtgatc
     5641 ttgatcgacg ctgaacggcg gagtgactgg ctggtgaagg cgatagacag acggctgcgc
     5701 gaggagttcg cgaagctccg cgtggagatc aacgaggaca agagtcggat ggtggatctg
     5761 acgaagggag agagcttcac tttcctgggt tttgaatatc ggcgtgtact gagccttcag
     5821 cggaagtggc gaccttacta tgcacccaag ctgaagaagc ggatagcgct gtttgaaaag
     5881 ctcagggaga tcttccggca gcatgtcagc tggcccgtca agaatgtgat tgagaagatc
     5941 aatccgattc tgcgtggttg ggtgaactat tttcgggtcg gccattcgag tctttgtttt
     6001 ggagtagtca aacgttgggt agagaagaag gtgcggcagc acttgatgcg tgcccgggga
     6061 cgcaaaggtt ttggctggaa gcggtggagt agcgaatggc tatatgatcg gctgggcttg
     6121 tttaatgact accagctccg tcgatggtcg tctgcgaaag tcgccccagc acaataggcc
     6181 ccataactct tgacaccaag caaacaggtg cgcccagtgc gggaaatccg cacgctgggt
     6241 gcgatgtggc gggggctgga aacggattca cggtttggct attgaggcac tcccagagga
     6301 aacggggagc aacaagctag gctaaacctt cggaacaccg cgccagtcct cgacccgacc
     6361 ggcttggcgc ctttgagtgg tcgcattgct cgaataaatg atctggctgg agatccatat
     6421 tcgcagatgc tctctgtatc gttgcatgtg gtcgcaggag ggcttccatg ccgcacgaag
     6481 cgcagccgga gtgcgtggtg atcccacggc ggggaacctg ccacggcaag caggatttgg
     6541 attacttcgc gggaatctcc gccgaaaagg ccggggcgaa agggctgtgc ctgcac
//
LOCUS       DASRKK010000620        12006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1130872, whole genome shotgun sequence.
ACCESSION   DASRKK010000620 DASRKK010000000
VERSION     DASRKK010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130872"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1084
                     /gene="coaE"
                     /locus_tag="VFC15_15025"
     CDS             470..1084
                     /gene="coaE"
                     /locus_tag="VFC15_15025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZM11517.1"
                     /translation="MIRVGLTGGVACGKSTVGAMLAQRGAHYLQADTLAHQLYAPGEP
                     TYHAVIEHFGQEILNPDGTINRGRLANIAFPNRIAELNAIVHPAVVDAQNQWMADAQR
                     LDPEGVAVVEAALLLEAGADKDFDQLIVVTCNLDQKVARYAQRAGISLDEARAEVQRR
                     SAAQLSDQDKATRADYVIDNSGTIEETIRQVDAVWTQLRKDSAH"
     gene            1175..2314
                     /locus_tag="VFC15_15030"
     CDS             1175..2314
                     /locus_tag="VFC15_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZM11518.1"
                     /translation="MKLVRPLLLGALIAGAFYWYTTHHRGVNPADYIARPTHVELTEA
                     AAPQTFDSEEQTNIEVYKRGIPSVVNITSTAVAFDFFYGPVPQEGQGSGFIIDKEGHI
                     LTNYHVIANAREVEVTLHNKKKYKATVIGRDRSHDLAVIKIDAPGLIAATLGDSKSLV
                     VGQKVFAIGNPFGLSGTMTRGIISSVRPIRTPEGGEIDEAIQTDAAINPGNSGGPLLN
                     SRGEVIGINSMIATGGSNQSAGIGFAIPINTAKAVISDLITIGRVRRPSLGIRILPIG
                     PEMAQELGLASDYGILIVQTVPGGSAERAGLHGGNERAYIGNVPLMVGGDLIVAIDGE
                     QIGDQQDLANLMNKHKAGETVTVTFFRGKKKMDLKVTLSEAREQT"
     gene            complement(2363..2575)
                     /locus_tag="VFC15_15035"
     CDS             complement(2363..2575)
                     /locus_tag="VFC15_15035"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZM11519.1"
                     /translation="MVGWGNDFRQPSADGLKSAEALWTIAVSLLLVWLVGIVSSSTFG
                     GALHLLLPLALVAYVVSRIEQERADS"
     gene            complement(2708..3049)
                     /locus_tag="VFC15_15040"
     CDS             complement(2708..3049)
                     /locus_tag="VFC15_15040"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HZM11520.1"
                     /translation="MGRIFSQLATGLALVVGTALLASTMWAQDAATQSQPQPGDQMQA
                     QQAATTRTFAGKIVKQGDKYVLRDVANKVTYMLDAQDDLKAYEGKEVTVIGSLDSASR
                     TIHVQKVQAPS"
     gene            3361..3720
                     /locus_tag="VFC15_15045"
     CDS             3361..3720
                     /locus_tag="VFC15_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11521.1"
                     /translation="MYDDFQAIDRWTAQPLHCQYQALIVAIATRHADAVDIKFLVGDR
                     PVWIALPHVAWVEFNRQTGKIITDPMAVQIAGHYLKWAIESGEDSGREMYPLTVQETL
                     DHLNAIVEENQASAVAG"
     gene            3850..5445
                     /gene="pckA"
                     /locus_tag="VFC15_15050"
     CDS             3850..5445
                     /gene="pckA"
                     /locus_tag="VFC15_15050"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZM11522.1"
                     /translation="MSSVTSIAHPELADLLAKKNIHRNLSTPKLVEIAIQNNEGVLSS
                     RGALVSETGKRTGRSPKDKFTVKDAITENKVDWGSVNLAFSPDKFDALYQRVLEYLAG
                     REIYVQDLFCGADPKYQLPIQVINEYAWHDLFVRQLFIRPTTEELKNFKPEFTIVAAP
                     GFLADPKRDGTNSEVFVIVNFTRKLVIIGGTQYAGEMKKSIFGVMNFLLPQNNVFPMH
                     CSANIGEDGVTALFFGLSGTGKTTLSADPTRRLIGDDEHGWSETGVFNFEGGCYAKCI
                     KLSEQNEPQIWNALTFGSVLENVVLDPVTREPDYDDDSKTENTRAAYPVEHIDNAVIP
                     GFGGHPKNVVFLTADAFGVLPPVSKLTPEQAMFHFMSGYTAKVAGTEAGVTEPQATFS
                     TCFGAPFMPLPPKVYAEMLGRRLREHNAQCWLVNTGWQGGPYGVGKRMSLPYTRAMVN
                     ALVEGNLDNVEFEIEPSFGLSIPKSVPGVPSDLLKPRNSWKDAAAYDKMAADLAARFE
                     KNFEQFDAPREIKEAGPRAVVAK"
     gene            complement(5637..6041)
                     /locus_tag="VFC15_15055"
     CDS             complement(5637..6041)
                     /locus_tag="VFC15_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM11523.1"
                     /translation="MKTYVFDASALFAFLQQKPGAPKVNELLKDAMRGRVAIFMSAVN
                     YGEVYGGILREHGAERARATMSAVQPLPISLVDATPQRSLQAAEVKVKHKLYYLDSFV
                     AALALEQKGTLVTGDSDFRRLGHSVPIVWLKT"
     gene            complement(6038..6283)
                     /locus_tag="VFC15_15060"
     CDS             complement(6038..6283)
                     /locus_tag="VFC15_15060"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM11524.1"
                     /translation="MKDISSTVTSKGQLVVPAELRRRHGIQAGTKVKFFEDQFGRIVL
                     QPITADYIDRVMGCLAEGPDLLATWEKEHRVEGKRGK"
     gene            complement(6390..6707)
                     /locus_tag="VFC15_15065"
     CDS             complement(6390..6707)
                     /locus_tag="VFC15_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11525.1"
                     /translation="MTTRAVFLLIALAIVGALVWAYHHDEELRKNAMLVNLGDPNEAV
                     RELLGNPTSEGSCGSLTVAPAACTDEYIYKYYYSVFQPQYEVVWFDSAGNVLGEQHVQ
                     HSF"
     gene            complement(6742..6936)
                     /locus_tag="VFC15_15070"
     CDS             complement(6742..6936)
                     /locus_tag="VFC15_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11526.1"
                     /translation="MKWLDSFSMRNFTNDQVFDDTIPVGDGLMEIGMRVPLERLRSSM
                     EDWFRRKSYLPKEGKLVVSE"
     gene            complement(7031..7885)
                     /locus_tag="VFC15_15075"
     CDS             complement(7031..7885)
                     /locus_tag="VFC15_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11527.1"
                     /translation="MKFILPGAVFVLMMSVGMSLRHDKMIAAFRRMTWSAWMRLLLAT
                     FIVPPAIALLLPHIFPLTLAEMAGLFMVGVAPGAPLMTRNIAKKGFDMHLAAGYQLWG
                     ALLIPVMIPLVVFATGKLYDRDIWIPPRVLLTEIAEKQFLPLLLGMALAYLLPVIAAK
                     LQPAMTVIGNVVLTVAIVLFLFKMGPVALKALTPWLPVAALVLAVGSVLAIRFLLRAD
                     VVTDRTLAICNANRHVGLALLLSGQYLHAKAALPAVAAYALVAPFVMGATSKWFHRGE
                     KAAAPAAA"
     gene            8091..9353
                     /gene="tyrS"
                     /locus_tag="VFC15_15080"
     CDS             8091..9353
                     /gene="tyrS"
                     /locus_tag="VFC15_15080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZM11528.1"
                     /translation="MPQFPPVDEQLDYLRKGAAEIIRESDLRERIEKSLASGKPMRVK
                     AGFDPTAPDLHLGHTVLMRKLKHFQDMGHTVIFLIGDFTGLIGDPSGRSATRPALTRE
                     QIDANAETYKAQVFKILDRDKTEVRFNSDWLGKLGFEGFIRLAAKFTVSQMLEREDFH
                     KRFVEEKPIAMHELLYPIAQGYDSVALEADVELGGTDQKFNLLMGRQLQRDYNQPPQI
                     VLTMPLLEGTDGVQKMSKSYGNYIGITEAPQEMFGKVMSISDALMWRYYELLTDVTVR
                     EIDELKQDVAKGAAHPMKLKQELGLRIVRDFHGQEAAEHAAQEWSRMFQKDEAPADLP
                     LVTVKAAEVLAPNAALPSDGLVQVKLDKLLFRTGLATSVADGLRKVKQNAVKVDGELK
                     NELVVALKLGSAVTLRAGKKMVRVSVEK"
     gene            9776..9979
                     /locus_tag="VFC15_15085"
     CDS             9776..9979
                     /locus_tag="VFC15_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11529.1"
                     /translation="MYGARARRRHSTLRFDLPPLDDMHVIQDLLSKLMGALCSDTIDY
                     RRATAMLSALRFASSELRNPREW"
     gene            10381..10701
                     /locus_tag="VFC15_15090"
     CDS             10381..10701
                     /locus_tag="VFC15_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HZM11530.1"
                     /translation="MRRWCLMWCCFLLSLVVWAEPSDSNPPKTIEGCVASINGAFMLG
                     TPSGERYILKGDHDALFSYNGKQVRITGTVKKSANGSSPSSPGEPRTLKVSDIKKVYD
                     TCQF"
     gene            10839..11015
                     /locus_tag="VFC15_15095"
     CDS             10839..11015
                     /locus_tag="VFC15_15095"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZM11531.1"
                     /translation="MAKSISCRDVGVDCDFTAKADTVDELMQVTAQHAKEAHGFDSIP
                     AELMPVVQAAIRDE"
     gene            complement(11101..11628)
                     /locus_tag="VFC15_15100"
     CDS             complement(11101..11628)
                     /locus_tag="VFC15_15100"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM11532.1"
                     /translation="MRARFVLSLLLCFGLSSFLFAQDAGTMTYAAAATSKFAPMAGLP
                     SCVTLSVQRGDPSKGPSVILLKFKPGCSIPWHWHTAAEQLMVVSGTGTAQMKDGKPVA
                     VHPGDYLYLPAKHIHTFAAVTAVTMFDLPDGPFDIHYVDASGNEIPPDQALKRNIAAT
                     PKITVKPAAAPVITQ"
BASE COUNT         2639 a         3535 c         3400 g         2432 t
ORIGIN      
        1 cactccgcct tcccactgca gccggggcac gtagacgttc aggtacatgc ggtcaatgca
       61 ctcgagctgg aaggtgatgt ggttttgcag aagggcagcg acactatgtg gtatgttcac
      121 tttcggctcc gtcggctgac cgctggtcct tgggaccaat taccgcctcg agcggtccat
      181 gtcacgaaca tgtttacaca tgttcgtatt tcggtcggtc gacggagccc gtaggccaac
      241 caaagcatga ctcgtgggtc tgaggcgaag cctcgttagg attagagcaa tttggatcag
      301 actgaagcct agttttcgtg cttaacaaga tattggtggg gtgaggtctc ctcagtggtg
      361 aaccgacagc cagcagcgag cctcagttta tactgcttat actgcgttgg atcgttgccc
      421 gagccatgca aagcctgtcg ccagccaatc ctataccgcg agaagcgcca tgatccgcgt
      481 aggtctcacc ggcggagtgg cttgcggcaa gtccacggtg ggtgcaatgc tggcccagcg
      541 cggcgcgcac tatctgcagg ctgacacact ggcgcaccag ttgtacgcgc ccggagagcc
      601 aacctaccac gccgtcatcg aacactttgg ccaggagatc ctgaatcctg acggaaccat
      661 caatcggggc cgactggcaa atatcgcttt tcccaaccga atcgcggaat tgaatgcgat
      721 tgttcaccca gcggttgttg acgcccagaa ccagtggatg gccgacgcac aacgcttgga
      781 tcccgaggga gttgccgtgg tggaagccgc gttgctattg gaagctggag ccgacaagga
      841 tttcgaccag ttgattgtcg tgacctgcaa tctcgatcag aaggtcgctc gctacgcgca
      901 gcgcgcgggg atttcgctcg acgaggcacg cgccgaagtg cagcgccgca gcgccgcaca
      961 gttatctgac caagacaagg ccacccgcgc cgattacgta atcgacaact ccggcaccat
     1021 cgaggagacg attcgccagg tcgatgcggt gtggacgcaa ttgcggaagg attccgcgca
     1081 ctaggcaccg cgcctacaat cccggtacac tttatccata agacacgtct aacgcagtct
     1141 gaaactctac ggaaaccggt gtatttctaa ccccatgaaa ctggtacgtc ctcttctgct
     1201 tggcgcgctg atcgcgggcg ccttctactg gtacacgacc catcaccgcg gagtgaaccc
     1261 ggcggactac attgcccggc ccacgcacgt cgaactgacc gaggctgccg cgccccagac
     1321 ctttgactcc gaagaacaga ccaacatcga ggtctacaaa aggggcattc catcggtggt
     1381 aaacatcact tcgaccgccg tggccttcga cttcttttac gggccggtgc cgcaggaggg
     1441 acaggggtcg ggcttcatca tcgacaagga aggccacatc ctcaccaact atcacgtgat
     1501 tgccaacgcg cgcgaagttg aggtcacact gcacaacaag aagaagtaca aagcaacggt
     1561 gatcgggcgc gatcgttcgc atgacctggc ggtgatcaag atcgacgctc ccggcctcat
     1621 tgcggccacg ctgggcgatt ccaaatcgct ggtcgtcggg caaaaagtat ttgccatcgg
     1681 caaccctttc ggactgagcg gcaccatgac gcgcggcatc atcagttcag tgcggcccat
     1741 tcgcacaccc gagggtggcg agatcgacga ggccatccag actgacgctg ccatcaaccc
     1801 tggaaactcc ggcggcccgc tgctgaattc gcgcggagaa gtcatcggca ttaacagcat
     1861 gatcgctact ggaggttcaa atcagagcgc gggcatcgga ttcgcgattc ccattaacac
     1921 cgccaaagcg gtgatcagcg acctgattac gattggacgc gtgcgccggc catcgctggg
     1981 aattcgtatc ctcccgattg gccccgaaat ggcgcaggag ttggggctcg cttccgacta
     2041 cggaatcctc atcgtgcaga cggttcccgg tggctcggcg gaacgggcgg ggctgcacgg
     2101 cggcaatgag cgcgcctata tcggcaacgt tccgctcatg gtcggtggag acctgatcgt
     2161 ggccatcgac ggcgagcaga ttggcgatca gcaggacctc gccaatctga tgaacaagca
     2221 caaagctggc gagactgtga cagtcacctt cttccgcggc aagaaaaaga tggacctaaa
     2281 ggtcacgctg agcgaggcgc gggagcagac gtagcgcgac ggcggtcgct cgtatcctca
     2341 ttctcggccg ggtcgcagcg agtcaggagt cggcgcgctc ctgttcgatg cgagaaacga
     2401 cgtaggcgac aagcgccagc ggcagcaaga gatgtagtgc cccgccaaag gtggacgagc
     2461 tgacgatccc caccagccac actaacaaca gactcaccgc aatggtccac agagcctcag
     2521 cgctcttcag tccatcagca ctcggttgcc ggaaatcatt accccatccg accatgacca
     2581 cactccatcc gcgcatacac gcgcgtttcg acccgaggca gaaggtggag ggacgaataa
     2641 aagtagcggc gggagcgagc tcagcatggg cccccttgag tgagaaccca tgccttagat
     2701 cctgcgctta ggagggtgcc tgcaccttct ggacgtgaat cgtgcgactg gccgaatcga
     2761 ggctaccgat cacggttact tccttgcctt catacgcctt caaatcgtcc tgggcatcga
     2821 gcatgtaggt gactttgttg gccacgtctc gcaatacata cttgtcaccc tgttttacga
     2881 ttttgccagc gaaggtcctg gtggttgctg cctgctgggc ctgcatctgg tcgccgggtt
     2941 gcggctggct ctgcgttgcc gcatcctgcg cccacatcgt tgatgccaac agtgcggtgc
     3001 cgaccacgag agctagtcct gttgcaagct gactgaatat tcttcccatt acgtttctcc
     3061 ctgatacctg aaagatcgat ttgtgcgcgc gtatgcgtag aagcaagttc agtgccaatt
     3121 gcgccgcaaa ttcatgactc cggaagtcca atgttttcag gccgaccggg gaccgttgga
     3181 tatgactgtg gaggttgaat tacgcaagat tttcctgaat cgggaagttt tttccagcgt
     3241 gatttagcgc ggcaggagaa catttgcggg gcgcaagcta gtcttacacc gcgacggagg
     3301 ccgctcactg caaccttcgg cattgctcca tccctgaccc tcagctatag ttttaaggtt
     3361 atgtatgacg acttccaggc aatcgaccgc tggaccgccc agcccctgca ctgccagtac
     3421 caggccctga tcgttgccat cgcgacccgg cacgctgacg ccgtggacat taagtttctg
     3481 gttggggacc ggccagtgtg gatcgcgctc ccgcatgtcg cgtgggtgga gttcaaccgg
     3541 cagacgggaa agatcatcac cgatcccatg gcggtccaga ttgcaggaca ttatttgaaa
     3601 tgggcgattg agtccggcga agatagcggg cgggaaatgt atcctctgac cgtgcaggag
     3661 acgttggacc acctgaacgc gatcgtcgag gaaaaccagg cttccgcagt cgctggatag
     3721 tgcctgttct acgggctttt tcattctgcg cgcggggagt tcgtggccct ctccccagca
     3781 atttctcgtc ctgagtttca gccgcctcac gtcacggcgg gttcgatttt tgataaggag
     3841 tcctcctata tgtcatccgt aacttccata gcacatccgg aattggccga cctgctggcg
     3901 aaaaaaaata ttcatcgcaa cctgtccact cccaaactgg tcgaaatcgc aatccagaac
     3961 aatgaagggg tactgagctc tcgtggcgcg ctggtttccg aaaccggcaa gcgcactggc
     4021 cgttctccca aagacaaatt tactgtgaag gacgcgatca ccgaaaacaa ggtggactgg
     4081 ggttcggtga acctggcgtt ttctcccgac aagttcgacg ccctctacca gcgggtgctg
     4141 gaatacctcg cgggcaggga aatttatgtg caggacttgt tctgtggcgc cgatccaaaa
     4201 taccagttgc cgatccaggt gatcaatgaa tatgcgtggc acgacctgtt tgtgcgccag
     4261 cttttcattc gaccgacgac cgaagagctg aagaacttta agccagagtt caccatcgtc
     4321 gctgcacctg gctttctcgc cgatcccaag cgcgacggca ccaactcgga agtcttcgtg
     4381 atcgtaaact tcacccgcaa gcttgtgatc atcggcggca cccagtacgc cggcgagatg
     4441 aagaagtcga tcttcggcgt gatgaacttc ctgctgcctc agaacaacgt cttccccatg
     4501 cactgctcgg ctaacatcgg cgaagacggc gtgaccgcgc tgttcttcgg actgtccggc
     4561 acaggaaaga cgacgctgtc agccgatccc acacgccgct tgattggcga cgacgaacac
     4621 ggatggagcg aaactggcgt attcaacttc gaaggcggat gctacgccaa gtgcatcaag
     4681 ctctcggagc agaacgagcc gcaaatttgg aacgcgctca cctttggctc ggtgctggaa
     4741 aacgttgtgc tcgatcccgt cacccgcgag cccgattacg atgacgacag caagacggaa
     4801 aacacgcgcg ccgcgtatcc cgtcgagcac atcgacaacg ccgttatacc ggggttcggc
     4861 gggcatccca agaacgtggt cttcctcact gccgacgcgt tcggcgtgct gcctccggta
     4921 tcgaagctga cgcccgaaca ggcaatgttc catttcatgt cgggctacac cgccaaggtc
     4981 gcgggcaccg aagccggcgt caccgagccc caggcaacat tttcgacctg cttcggcgcg
     5041 cccttcatgc cgttgcctcc caaggtttac gcggagatgc tgggccgccg cctgcgtgag
     5101 cacaacgcgc aatgctggct ggtaaatacc ggatggcagg gcggaccgta cggcgtcggc
     5161 aagcgcatga gcctgcccta cacccgcgcc atggtcaacg cgctggtcga aggcaacctg
     5221 gataacgtgg agttcgagat cgagcccagc ttcgggctga gcattccgaa gtcggtgccg
     5281 ggagttccgt cggacctact gaaaccccgc aactcgtgga aagatgcggc tgcctacgac
     5341 aagatggcgg cggacctggc ggcacgtttc gagaagaact tcgagcagtt cgacgctccc
     5401 cgcgaaatca aagaggcagg gccgcgggca gtggttgcga agtaggcgat tagtaccaca
     5461 atgtaggatg tctgacagag tggcatcgct tcgaaacggc tgtggaaata cgcgatttgg
     5521 tggaagagtt ggcctttggg ccagcggctg agccacctat ttattatctc cttcgcggac
     5581 tttagttcgc gaaggacatc ccttaccgct ctttccgtgt tcagttcaga cagctttcac
     5641 gttttcaacc agacaatggg aacgctgtgg ccgagccgcc gaaaatcgga atcgccggtc
     5701 accagcgttc ccttttgttc gagtgcgagc gccgcaacaa agctgtcgag ataatagagt
     5761 ttgtgcttca ccttgacttc ggcagcctgc aatgacctct gcggcgtggc atcgactaag
     5821 ctgatcggca acggctgcac ggcgctcatc gtagcccgtg cccgctccgc accatgttcg
     5881 cgaaggatcc cgccgtacac ctcgccgtaa ttcactgccg acatgaagat cgccacgcgt
     5941 ccccgcattg catccttcag caactcattc accttgggcg ctccgggctt ctgttggagg
     6001 aacgcaaaca gcgcactcgc atcgaaaacg taggtcttca ttttccgcgc ttgccttcca
     6061 cgcggtgttc tttctcccag gttgccagca ggtctggtcc ttctgccaaa cagcccataa
     6121 cgcggtcgat gtagtccgcc gtgatcggct gcagcacaat gcggccaaac tggtcttcga
     6181 aaaacttgac cttggtgcca gcttgaatcc catgcctgcg gcggagctcc gccgggacca
     6241 caagttggcc ttttgaggtg acagtagaac ttatgtcctt catgtcttac atgatatcaa
     6301 atgtaagacg tgtgttgagt tcggcgcaca tgcgcgaact gccgctgtcg ttcaaactgg
     6361 caactggcta ctgagaactg acagcggctt cagaacgagt gctgcacgtg ttgctcgccc
     6421 agcacattac cggcgctgtc gaaccacacc acttcatact gcggttggaa caccgagtag
     6481 tagtacttgt agatgtactc gtccgtgcat gcagcaggag cgaccgtaag cgacccgcag
     6541 gagccttcgc tggtcgggtt tcccagcaac tcgcgcaccg cctcgttggg atcgccgagg
     6601 ttcaccagca tggcattctt ccgcaactcc tcatcgtggt gataagccca gacgagcgca
     6661 ccaacaatgg ccagcgcgat caagagaaat acagcgcggg tagtcataac agaagtttca
     6721 agcttcacta accactaacc actattcact aaccactagt ttgccttcct ttggtaaata
     6781 gctcttcctg cgaaaccagt cttccatcga cgaccgcagc cgctccaacg gcacccgcat
     6841 gccgatttcc atcagcccgt cgccgacagg aatcgtatcg tcgaacacct ggtcgttggt
     6901 gaagttgcgc atgctgaagc tatccagcca cttcattgga cacggcttcc gctccccgtt
     6961 ctcttcgtac tccactcgta tcttgcgcgc gaacataggt cgattgtgcc acgggtgaat
     7021 gtggattaca ctacgccgcc gcaggtgccg ccgctttctc gccccgatga aaccatttcg
     7081 aagttgctcc catcacaaac ggagccacca gcgcgtacgc cgccacggcc ggcagcgcag
     7141 ccttggcatg cagatactgt ccactgagca gcagcgccag tccgacatgg cggttggcat
     7201 tgcagatggc aagcgtgcgg tcggtcacaa cgtcagcccg caacaagaag cggatcgcca
     7261 gcaccgatcc taccgccagc accagcgccg ccaccggaag ccacggcgtc agcgccttca
     7321 gtgccaccgg tcccatcttg aacaagaaca gcacgatggc cactgtcagc accacgttcc
     7381 cgatcacggt catcgcgggt tggagcttgg cggcaatcac aggcagcaaa tacgccaggg
     7441 ccatgcccag cagtagcggc agaaattgtt tctccgcgat ttccgtcagc agcacgcgcg
     7501 gcggaatcca gatatcgcgg tcgtagagct tgcctgtagc gaacaccacc agcggaatca
     7561 tcaccggaat cagcagtgca ccccacaact ggtatcccgc ggccaggtgc atgtcgaagc
     7621 ccttctttgc gatgttgcgc gtcatcagcg gagcccccgg cgccacgccc accatgaaca
     7681 ggccagccat ctccgccagc gtcaggggaa agatgtgcgg cagcagcagc gcgatcgccg
     7741 gaggcacaat gaacgtcgcc agtagcaacc gcatccacgc ggaccatgtc atacgccgga
     7801 acgccgcgat catcttgtca tgccgcaggc tcatgccgac cgacatcatc agcacaaaga
     7861 ctgctccggg taggatgaat ttcattgttc ggtcattaga acatccgccg agaagtttcg
     7921 gaatacctgt catacgctgg gtgccccgtt ctaccggccc tcttttggcc ggtatggcgg
     7981 gatttcgtac tctacatgtt ctcaatcccg attcgcgtca tccacgcaaa tccgcggtcg
     8041 cttttcccca gttaccagtc accagtcacc cgattttcta taatcaatga atgcctcaat
     8101 tcccaccggt tgacgagcaa ctggactacc tgcgcaaggg cgcggccgaa atcattcgcg
     8161 agagcgacct gcgcgagcgc atcgagaagt cgctcgcctc cggcaagcca atgcgggtga
     8221 aggccggatt cgatcccacc gctcccgacc tgcatctcgg acacaccgtg ctcatgcgca
     8281 agctcaagca cttccaggac atgggccaca ccgtcatctt cctcatcgga gacttcaccg
     8341 gcctcatcgg cgatccctcg ggccgctcgg ccacgcgccc ggcgctcacc cgcgagcaga
     8401 tcgacgccaa cgccgaaacc tacaaggcgc aggtattcaa gatcctcgac cgcgacaaga
     8461 ccgaagtgcg cttcaacagc gactggctgg gcaagctggg cttcgaaggc ttcatccgcc
     8521 tggccgccaa gttcacggtc tcgcagatgc tcgagcgcga agacttccac aagcgcttcg
     8581 tcgaggagaa gcccatcgcc atgcacgaac tgctctatcc catcgcgcag ggatacgact
     8641 cggtggcgct cgaggccgac gtcgaactcg gcggcaccga ccagaagttc aacctgctca
     8701 tgggacgcca actgcagcgc gactacaacc agccgccgca gatcgtgctc accatgccgc
     8761 tgctcgaagg caccgacggc gtgcagaaga tgtcgaagtc ctacggcaac tacatcggca
     8821 tcaccgaagc gccacaggaa atgttcggca aggtgatgtc catctccgac gcgctgatgt
     8881 ggcgctatta cgagctgctc accgacgtca ccgtgcgcga gatcgacgag ctgaagcagg
     8941 acgtggcgaa gggcgcagcg catcccatga agctcaagca agagctcggc ctgcgcatcg
     9001 ttcgcgactt ccatggacag gaagccgccg aacacgccgc acaagaatgg tcgcgcatgt
     9061 tccagaagga cgaagctccc gcagacttgc cgctggtgac ggtgaaggcc gcagaagtgc
     9121 tggctccaaa tgcggcgctg ccttcggatg gattggtaca ggtgaaactc gacaagctgc
     9181 tctttcgcac gggacttgca acgtcggttg cggatggact gcgcaaggtg aagcaaaatg
     9241 cggtgaaagt ggatggagaa ctcaagaatg agctggttgt ggcgttgaaa ctggggtctg
     9301 cagtcacgtt aagggcggga aaaaagatgg tcagggtgag tgtggaaaag taagatgacg
     9361 agtgccgttg ggataaccag gacacgggca tgacccaaaa ctgcgagaca gctagttcgc
     9421 cagataccgc ccgcaaccgc gactgcagcc ctccgtcgat actttacaaa tatttctcgt
     9481 cagcgcgttt ggatgtcctg gcagagagga gtgtccgatt cacacaacca agtgacttca
     9541 acgacccttt tgagtttcgg cccaggattc aggcggcagc acatgagcac gaggtccgga
     9601 ctcacgtgga aaataacttc gatcgattga tcgaccaaga gttaaacaag tacggcgcgc
     9661 tagtccctcc cgagttgctc gacgcagtga gggcggcggc gcacgatcag aaatttctcg
     9721 tccccggcac ggccagaaat tttgttactt ccacactcgc cagcgccacg atgccatgta
     9781 cggagcccgg gcccgccgac gccactccac cctccgcttc gacctgcctc cgctcgacga
     9841 tatgcacgtc atccaggatc tgctgtccaa actgatgggc gcactgtgct cagacacgat
     9901 cgattacagg cgtgccaccg ccatgctctc cgccctgcga ttcgcctcca gcgagcttcg
     9961 caaccctagg gagtggtaaa aaagatggcc atgcaactcc ttgcggatca atattttacg
    10021 tgcaagtaat tgacagcaca gattttgcca ggaaattctc cctatcttga tgaaactgca
    10081 gattttgtgc agcatgaatt aataatcgcc aatgcgggct accacctcat cccggcctac
    10141 cgtggttgcg ccgtgactgc accgaatatc ctcagggagg ctaaattaac ctcctaagtc
    10201 cttacatctg acatggatag ctataagttc tatttaaatc aatgagatgc aaacaattgc
    10261 aggctaagtc aatgaaaaca aagggagcct gataagaaaa tttggcgcgg caaccgaatt
    10321 agcgaactcg tacgcgtttg cgatgacgat ataatctgcc ggatgtgtaa ggagccgaac
    10381 atgcgcaggt ggtgcctgat gtggtgttgc tttctgctgt cgctggtcgt ttgggctgag
    10441 ccgtcagaca gcaatccgcc gaagacgatt gagggctgcg tggccagtat caatggggcc
    10501 ttcatgctgg ggactcccag cggcgaacgc tatatcctca aaggcgatca cgacgctctg
    10561 ttcagttaca acggaaagca agtccgcatc accggaacgg tgaagaagtc tgcgaatggt
    10621 tcgtcgccgt cgtcgccggg cgagccacgc acgctgaagg tgtcggacat caaaaaggtt
    10681 tatgacacct gccagttcta gtggggtcgc gccgttcact ggccgttcac tggccgatct
    10741 tgtcaggcgt ggaagcgagg gtgtagagtg acacgcacgg cttgcctcta ggatggatta
    10801 agccgacacg caaactttgt aggggagagg aatcaatcat ggcaaagtcg attagttgca
    10861 gggatgtggg ggtcgattgc gatttcaccg ccaaggccga cacggtggac gaactgatgc
    10921 aggtgacggc gcagcacgct aaagaagcgc atggcttcga ctcgattccg gcggagttga
    10981 tgccggtggt gcaggccgcc atccgcgacg aataacggat tcagcgcaga ccgacgaaat
    11041 tgcggcgcac cgagagtgcg ccgtttttgc tgccgcgaaa accagtttct gtggttggcg
    11101 ctattgcgtt atgaccggcg cggctgcggg tttcacggtg atctttggcg tcgctgcgat
    11161 gttgcgtttc agcgcctggt ccggcggaat ctcgttgcct gatgcgtcca catagtggat
    11221 atcgaaaggc ccgtccggca aatcgaacat ggtcacagcg gtgacggccg cgaacgtatg
    11281 gatatgcttg gcgggcaggt acaagtaatc gccggggtga acggcgacgg gcttgccatc
    11341 tttcatttgg gcggtgcctg ttccgctaac caccatgagt tgctcggcag cagtgtgcca
    11401 gtgccaggga atcgagcatc ccggcttgaa cttaagcagg attaccgaag gccccttgct
    11461 gggatcgccg cgctggacgg aaagcgtcac gcaggacggg agtccggcca taggtgcgaa
    11521 cttgctggtc gcggcggcag cgtaggtcat ggtgccggcg tcttgcgcga acaggaaaga
    11581 gctgagcccg aaacacaaca gcaaactcaa gacaaatcgt gcgcgcacgg tgcacctccg
    11641 ttggagtgaa atgtgaaacg cgcgctagca taacgcagaa atatccgaac gtaaacagtg
    11701 ttattgcggg ttgtcaacgg acattagaaa tgtccccttg cttaagacat tagaaatgtc
    11761 cccttttgtc gtggtggtgc atttttattg tccagtagta gttgaggctg tgcgtaaagt
    11821 ggaaatccgc gttgtgtgcg gatttccaag cgtagtggga aagttcgttt tgtggacttt
    11881 tccacggagc ggcttttcca cagccttgcg ccgccgcgct tcgcgtgatt gttcttcggc
    11941 ttccgccatt ttgctcctac ctcgcttagc aggtggtcta agtttctcaa cctcgatagg
    12001 gcgaag
//
LOCUS       DASRKK010000621        14166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_251630, whole genome shotgun sequence.
ACCESSION   DASRKK010000621 DASRKK010000000
VERSION     DASRKK010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251630"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFC15_15105"
     CDS             <1..195
                     /locus_tag="VFC15_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZM11533.1"
                     /translation="IYGMPIIDVDKAHTVMVIKRSMAPGFAGIDNPLYYLDNNLMLFG
                     DAKQFVGNIVKELSGNSGGH"
     gene            393..935
                     /locus_tag="VFC15_15110"
     CDS             393..935
                     /locus_tag="VFC15_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11534.1"
                     /translation="MISWESFRHQNTAYDLTHLHPRIVIFTQAAKGDRPERTYTVNVA
                     FGLHCFTRGLKENEQPDPALSYSDSREARIFDFERFELSKRLPALVQNLPQSKCFHTG
                     HANYFSIEVLNDQGHAIEYEIYFAVTRSGKKGGRLTLFVQSAYVRDDKHGLNKPQKKA
                     ISFYVILFNTLNDRPIKPAP"
     gene            complement(1405..1869)
                     /locus_tag="VFC15_15115"
     CDS             complement(1405..1869)
                     /locus_tag="VFC15_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11535.1"
                     /translation="MPLAPQEIRTFFITAATWGRRPIFCAEPMAQLFLDTLQRYRAQH
                     KFLLHEFVVMPDHFHLLLTPAPEVPMEKTVQLIKGGFSFRVKKELNSNLEVWATGYTE
                     HRVKDAADYQQHAVYIRENPVRARLVETCAAYRFSSASGEWEIDPTPPWLKP"
     gene            2043..2255
                     /locus_tag="VFC15_15120"
     CDS             2043..2255
                     /locus_tag="VFC15_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11536.1"
                     /translation="MKNKQYGASLELRKLYQVLPDDEAAKRDQLRVIDESGEDYLYPE
                     NYFVPVNLPQAAEKAVFRVAGLASAK"
     gene            2348..2500
                     /locus_tag="VFC15_15125"
     CDS             2348..2500
                     /locus_tag="VFC15_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11537.1"
                     /translation="MLTDEDKQWIKQQLEGVETTLLTEFHEWASPVEMRQRSHAAAIR
                     ALDSEV"
     gene            complement(2642..2854)
                     /locus_tag="VFC15_15130"
     CDS             complement(2642..2854)
                     /locus_tag="VFC15_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HZM11538.1"
                     /translation="MGALRDQIANLTSAEKAELLDEVWESLEADALTLTDAQRVELDS
                     RIERLEKNPSDVIPWEKVRSDLFKRP"
     gene            complement(2909..3772)
                     /locus_tag="VFC15_15135"
     CDS             complement(2909..3772)
                     /locus_tag="VFC15_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZM11539.1"
                     /translation="MKFKPFLARPGKQFRLKDYDPGYTGHYDSEESAKAELQENVERL
                     AKYQDLLYADSTYALMVVLQAMDAAGKDGTIKHVMSGVDPSGCEVTAFKPPSEEELHH
                     DFLWRCYRRLPQRGLIGIFNRSYYEEVLVARVHPEVLEHQRLPGFTKPDRKFWKQRFE
                     SINDMEHHLVRNGTLVLKFFLYLSKEEQRKRFLERIEDPRKNWKYSDADLHERLCWDD
                     YTDAFEDAIQHTSTKHAPWYVIPADNKWFTHLTVSDIIVETLKDLKLAYPKMDDEQRK
                     RLEQAKKALMG"
     gene            complement(3887..5221)
                     /locus_tag="VFC15_15140"
     CDS             complement(3887..5221)
                     /locus_tag="VFC15_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM11540.1"
                     /translation="MVRAGQLVVLTILLSSVLVFGQTSAPGASSTAVPANQSGPAFDV
                     KAAVDTYLAKMPPAQRARSDAYFEGGYWLQLWDLLSTVVVMWLLLRFGWSARMRNLAE
                     RITRFRPLQTALYWVQFIVVVSVLTFPMGVYEGYFREHKYGLLNQTFGPWMRDQAVGL
                     GVGVVLGAILMVPLFGLVRRLGKNWWVWGAIVMMVFVAFVSLIAPVYIAPLFNKYTTL
                     TDARIKDPILSMARANGIPANDVYEFDASRQSNRVSANVSGFAGTMRISLNDNLLKRC
                     TLPEIETTMGHEMGHYVLNHAYKGLVMFGVVIVIGFAFVNWGINYSLARWGGQWNVRG
                     ITDVAVLPLAVIVFSVYMFLVTPVTNTISRNMEFEADMYGLNAAQQPDGEANVDMLLG
                     EYRKLDPGSVEEFMFYDHPSGRTRITAAMRWKAEHPESANQEEMARPLFEGK"
     gene            5952..6155
                     /locus_tag="VFC15_15145"
     CDS             5952..6155
                     /locus_tag="VFC15_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11541.1"
                     /translation="MFTFLLWIIVLIFCWPLALVALVLYPIVWLLLLPFRILGIAVGG
                     VLELVKAIILLPARILRGPSRAF"
     gene            6290..8023
                     /locus_tag="VFC15_15150"
     CDS             6290..8023
                     /locus_tag="VFC15_15150"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZM11542.1"
                     /translation="MNTTNHAMETPATSPSEAPKASNFIRDIILADLESNKFGGRVQT
                     RFPPEPNGYLHLGHAKSIYLNFGLASEFGGGTNLRFDDTNPEKEEQEYVDSIVRDVRW
                     LGYEWAGLYYASDYFDQLYAWALQLIKVGKAYVCDLNAEEVRQYRGTLTEPGKESPYR
                     DRTVDENLDLFERMHAGEFEDGSRTLRAKIDMASPNLNMRDPVMYRILHAAHNRTGDK
                     WCIYPMYDYAHGQSDSMEKITHSICTLEFEDHRPLYNWFIEQLGIFPSQQIEFDRLNV
                     TYTLLSKRKLLQLVQEGRVRGWDDPRMPTLSGIRRRGCTPEALRNFCAAIGASKTNGT
                     IELAMLAHYVREDLNRRSLRAMAVLRPLKVVIDNYPEGQSEELQLVNNQEDPSAGTRA
                     VPFSQVLYIEQDDFREDPPKKFFRLSPGREVRLRGGYFIKCTGVVKDATGAITEVHCT
                     YDPETRSGNAPDGRKVKATIHWVSGEHSIDAEVRIYENLFVKEDPSEVEGDNTFLSNL
                     NPNSFEILTDCKLEPSLASAKPGERYQFERMGYFCVDPDSTAERLVFNRTVALRDTWA
                     KIEHRDKASTA"
     gene            complement(8050..8985)
                     /locus_tag="VFC15_15155"
     CDS             complement(8050..8985)
                     /locus_tag="VFC15_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM11543.1"
                     /translation="MRRLAFIVLLCVAAVCVAQDQDFSKVQIKVTKVSGNIYMLEGAG
                     GNIAASVGEDGIVIVDDEYAPLADKIAAALKAIGVTDKPVRFVINTHYHGDHTGGNLA
                     FATGGSTIIAQDNVRKRLESGGKAGNGASVAMDVKPAAKGALPIITFDHDVTVHLNGD
                     DIRALHFPAGHTDGDAIIFFPKANVVHMGDDYVRYGYPFIDVTAGGSVQGMIAACEKV
                     AAMLPADVKVIPGHGQISNLNEVREYVTMLKETSAAVQAAIKSGKSVDQMKKEKILAA
                     WDKKYSGDFINSDVFIETLYNSLTNQKNAEFVKHN"
     gene            complement(9062..10009)
                     /locus_tag="VFC15_15160"
     CDS             complement(9062..10009)
                     /locus_tag="VFC15_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11544.1"
                     /translation="MRQMVTAAFLVLCASAMGTSKPITKVQLPCESRYQDLSPTGTQL
                     VVHCKDSSVRVVNVPEGTEQHVFSADQHANSVVYSQDGSWLAVGFDDGTVELFSSPGA
                     TSSKRWKASSRRIDMMYFFPDAKSLVVGPVDSPGQVWEIANTPTLRATLPFEFGGLIA
                     CAVSPDRKLLVAAGDDTVLRWYDTATWKKRRENSDFLLETFALTFTPDGKHVLAGGAD
                     ARITVLDAATAKQLRQFPPETGSSVAAINVLGDKQHAASVYFDDAGEKPPHAMLWDIA
                     SVKSAALQSDAPLTCGAVVGGKLWVCSADGKTLTMSQYE"
     gene            complement(10009..10635)
                     /locus_tag="VFC15_15165"
     CDS             complement(10009..10635)
                     /locus_tag="VFC15_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11545.1"
                     /translation="MHFTRRELLERCAAFGAITLATSLPLSALATDWDEAEKKKQPTP
                     FCELGPFYKREAPHTSVMRAAGDSGMPLTIAGGVYNTRGEIIPNAKVEIWQTDNDGHY
                     DVEGYRYRAVLEPGAKGSYAIESVMPGHYPGRVCQHVHYLITAPGHKPLITQLYFATD
                     PVFDGNPEKNFTRDPLITSRELVRPVVIKGDPKQIIAAVNFDLVMETL"
     gene            complement(10707..11648)
                     /locus_tag="VFC15_15170"
     CDS             complement(10707..11648)
                     /locus_tag="VFC15_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZM11546.1"
                     /translation="MAAIILDGTKIAAQIRAEVGEEVKQLTADGLRPGLAVVLVGHNP
                     ASEIYVRSKVKACEELGIMSEKITPPESSTTEELLALVVDLNRRDDIDGILVQLPLPA
                     HVDAKKVLLAVSPDKDVDGFHPMNVGYLSTQRPGLVPCTPAGVIEILKRSNIAIAGAE
                     AVVVGRSDIVGKPVAMLLLNGNATVTVCHSKTRDLPGVCRRADILVAAIGRTGMITPE
                     YVKPGATVIDVGMNKITDPVEFQKFFAGDAKREQSFKKNGSTLIGDVHPKVAEVAGAL
                     TPVPGGVGPLTIAMLMSNTVKAAKLRRGSKVAAAAAR"
     gene            12433..12657
                     /locus_tag="VFC15_15175"
     CDS             12433..12657
                     /locus_tag="VFC15_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11547.1"
                     /translation="MAEKFSVPELAALRSELLLGFDSKDAAELVQLFLAGHGYGVSPE
                     AARYAVSRVGLMGNTLEVFQRELESIALAM"
     gene            complement(12932..13699)
                     /locus_tag="VFC15_15180"
     CDS             complement(12932..13699)
                     /locus_tag="VFC15_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZM11548.1"
                     /translation="MSIRVLLSDDEALARERLRGLLQKEPDLDIVAECGDGKSAIALI
                     QREMPDLVFLDVQMPEVDGFGVVKELEKQALLPLTIFVTAYDRYAMRAFEVHALDYLL
                     KPVNQERLGEALDHARKQLMHPSEAMFQKRVLEMLGGLDSRRHAPDRIVVKADGEIVC
                     LKPNEIDWVESAGNYACLHVGNVTHILRETITALENRLGARQFLRVHRSTLVNVDRIK
                     TLKPSLYGDYAILLRDGTKLTLSRGFRESVLKRLGTA"
     gene            complement(13677..>14166)
                     /locus_tag="VFC15_15185"
     CDS             complement(13677..>14166)
                     /locus_tag="VFC15_15185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM11549.1"
                     /translation="LDGVGVHEVPLHQELMFLQKYIDIQQTRFHDRLRVETDIDPRTL
                     DTLVPNLILQPLVENAIRHGIGPRRAPGLIRIATWRDNDDVWMEIRDDGTGLMRGRGV
                     LPPEGVGLRNTRGRLQQLYNENHAMVLEDAPGGGCTVKIRVPYRSKNEEVTNPNVNSS
                     VA"
BASE COUNT         2989 a         4152 c         3975 g         3050 t
ORIGIN      
        1 atctacggca tgccgattat cgatgtcgac aaagcgcaca ccgtgatggt catcaagcgc
       61 agcatggccc cgggcttcgc cggcatcgac aacccgctct actacctcga caacaacctc
      121 atgctcttcg gcgacgccaa gcagttcgtg ggaaacatcg tgaaggagct gtcgggcaat
      181 agcggggggc actagcaggt tgctgaaaga gtcgctcgag gacgcggtcg aggactattc
      241 ttgttcgaac gggggacggg cgccattagt caagggcatg atgatgatga tcgtggcgtc
      301 ggaatagtag tggtagctga ctttctattt ctgcttaatc tacttcaaag cgggactgtg
      361 gtctaatgaa gtagcagcgc gtctggcccg gtatgatctc ttgggaatct tttcgtcatc
      421 agaacacggc ctacgaccta acccatctcc accccagaat cgtaatcttc acacaagcgg
      481 cgaaaggaga tagaccagag cgcacataca cggtgaatgt ggcgttcggt ctgcactgtt
      541 ttactcgcgg cctaaaggaa aatgagcaac ccgatccggc actcagctat tcagattcga
      601 gagaagcacg aatatttgat tttgaacgtt ttgaactttc aaaacgtctt cctgcacttg
      661 tccaaaacct tccgcagagc aagtgctttc acacgggaca cgcaaactac ttttccattg
      721 aagtactgaa tgaccaaggt cacgccattg agtacgagat ttattttgcg gtgactcgat
      781 ctggcaagaa gggcggcagg cttacgcttt tcgtgcaaag tgcgtacgta cgagacgata
      841 aacacggttt gaacaagcct cagaagaaag ccatatcgtt ctacgtgatc ctgttcaaca
      901 ccttgaacga ccgcccgatt aaaccagcgc catagcaaaa ggctctcctg ttaagaagag
      961 ccttctttta gaattgattc aatcgggcct cttggatatc cccacacttg ggtgggtgcc
     1021 attgctggcg ggaccaacgc acggcatcta acgccgctcc gttcatacgt tggcatcccc
     1081 atgctatccc gaccagcggg agaagtcaag ctttttcttc gcccacaaca gctcggtgca
     1141 acgccttaat tatacacagt ttataaccga tcggcggttg ccgcaggtaa cccctcccat
     1201 tgaccaagaa tttcacgtcg cgcgccccat ttcaagcggc ccgtctttgg cccttgagtg
     1261 ggggttttca tctcgatcct gcgacaatgt cgaatacatt cgagcccgca ggacgatatt
     1321 tgtttgggac gtggggcgtc agcctcaaga tttgacgagg gtggaagcac cacgctttag
     1381 tggtgaaaaa cggctccgtc gtttctatgg ctttagccat ggcggggttg ggtctatctc
     1441 ccactcgccc gacgccgagc tgaaccgata cgccgcacac gtctccacca atctcgcgcg
     1501 gaccgggttc tctcgaatat aaacagcgtg ctgctgatag tcggccgcgt ctttcactcg
     1561 atgctctgtg tatccggttg cccagacctc caggttcgaa ttcaactcct tctttacgcg
     1621 aaaggagaag cctcctttga tgagttgaac tgtcttttcc atgggcactt caggcgcagg
     1681 cgtcaagagc agatggaagt gatcaggcat gaccacaaat tcgtggagca ggaacttgtg
     1741 ctgcgcacga tagcgctgca gcgtgtcgag gaatagctgc gccatcggtt cggcgcaaaa
     1801 gatgggccgg cgcccccacg tggcagcggt gataaaaaac gtgcggatct cttgcggcgc
     1861 aagcggcata cgttccgagg ctaaagcctg agtatattcc gccggctttt caccacgcta
     1921 aagcgtggtg cttccacccg tcactggtac gaagcgcatg gaatcggcgc caaggaacac
     1981 aaggtcaagc gtattcttga ctcacaacta tgaaacgtgc ggaatcacaa ttcgttgttt
     2041 gcgtgaagaa caagcagtac ggcgcatcgt tggaactgag aaagctctac caggttcttc
     2101 ccgacgatga ggctgccaag cgtgaccaac tccgcgtgat tgacgagtcg ggagaggact
     2161 acctgtatcc cgaaaattac tttgtaccgg tgaatctgcc ccaggcggca gaaaaggccg
     2221 tgttccgcgt ggcgggcctc gcatcggcaa agtaattgac cggacccggc cacccgcgtg
     2281 gagattgttt ggaaatgaac tggcgcgggg tgggcgacct gccctgggtt gcccctataa
     2341 tcaggctgtg ctgactgacg aagacaagca gtggatcaag caacagctcg agggggtaga
     2401 gaccaccctg ctcacggagt ttcacgaatg ggcttcgccc gtcgaaatgc gccagcggag
     2461 ccacgcagcc gcgattcggg cgcttgactc cgaagtgtag tcgctcgccg accggctaaa
     2521 gaatttggaa gggcgctgag ctgcggttgg cgtgaagtgc gtgcccgact cgggctgttt
     2581 tcgcccgggc gactattctt ggttggacga atgcccccgc cccgccaaac gagggctagc
     2641 ttcacggtct cttaaacagg tccgacctga ctttttccca gggaatgaca tcggaggggt
     2701 tcttctcgag gcgctcaatg cgggaatcca actcgacgcg ttgggcgtcg gtcagcgtga
     2761 gagcgtcagc ctccaggctc tcccacactt cgtccagcaa ttccgccttc tcggcagacg
     2821 tgaggttggc gatctggtct cgtagcgcgc ccattgccta taaccctagc gcggtttcct
     2881 ggtccccgca agcccgcagg ggcggcggtc agcccatcag tgccttcttt gcttgttcca
     2941 ggcgcttgcg ctgctcgtca tccatcttgg gatatgcgag cttcaggtct ttcagggtct
     3001 ccacgatgat gtctgacact gtgaggtgcg tgaaccattt gttgtcggcc ggaatcacgt
     3061 accagggcgc gtgcttcgtg ctggtgtgct ggatcgcatc ttcgaaggca tccgtgtaat
     3121 cgtcccaaca caagcgctcg tgcagatcgg cgtcggagta cttccaattc ttgcgcgggt
     3181 cttcaatccg ctccaggaag cgtttccgct gttcttcctt ggataagtac aggaagaact
     3241 tgagcacgag agttccgtta cgcaccaggt ggtgttccat gtcgttaatg gactcaaatc
     3301 gctgcttcca gaacttgcga tcgggcttgg tgaagccggg caagcgctga tgttcgagga
     3361 cctccggatg aacgcgggct acgaggacct cctcgtagta ggagcgattg aagatgccga
     3421 tgagcccgcg ttgcggcaga cggcgataac agcgccagag gaaatcatgg tgcagctcct
     3481 cctccgacgg cggtttgaat gcggttactt cgcagccgct tgggtccacg cccgacatga
     3541 cgtgtttgat ggtgccgtcc ttgccggccg catccatcgc ctgtaacacg accatcaacg
     3601 cataggtgct gtcggcatag agcaggtcct gatacttggc cagacgctca acgttctcct
     3661 gcagctcggc cttggcgctc tcctccgagt cgtaatgacc ggtgtatccg ggatcgtagt
     3721 ccttgaggcg aaattgtttg ccgggtctgg ccagaaacgg tttgaatttc atatatgcga
     3781 gatgatacgc gcccccagac ttgggggttg acccacctgg agggggaagg agtatcctcc
     3841 ctgtgctccg actgttccct tcctctggaa aagggcggga agaatctcac ttcccctcga
     3901 acaacggcct tgccatctcc tcctggttcg ctgactccgg atgttccgcc ttccaccgca
     3961 tcgcggcggt gatgcgggtg cgtccgctgg gatgatcgta gaacatgaac tcctccaccg
     4021 aaccgggatc cagcttgcga tattctccta gcaacatgtc cacattcgcc tcgccgtcgg
     4081 gttgttgggc cgcatttagg ccgtacatgt ctgcctcgaa ttccatgttg cgcgagatcg
     4141 tattggtcac cggtgtcaca agaaacatgt aaaccgagaa cacgatcacc gcgagcggca
     4201 gcacggcgac gtcggtgatt cctcgcacgt tccactgccc gccccagcgc gcgaggctgt
     4261 agttaatgcc ccagttcacg aaggcgaacc cgatcacgat caccactcca aacatgacca
     4321 gtcccttgta cgcgtggttc aggacatagt ggcccatctc gtgtcccatg gtcgtctcaa
     4381 tctcgggcaa cgtgcagcgc ttcagcaggt tgtcgttcaa cgagatccgc atcgtcccgg
     4441 caaatccgct gacgttggcg ctcacccggt tcgattgtcg cgaggcgtcg aactcgtaca
     4501 cgtcgttggc aggaatccca ttcgctcgcg ccatgctgag aatcggatct ttgattcgtg
     4561 cgtccgttag cgtcgtgtac ttgttgaaca gcggagcgat gtaaaccggg gcaatcaggc
     4621 tcacgaacgc gacaaacacc atcatcacga ttgcgcccca cacccaccag ttctttccca
     4681 accgtctcac cagcccaaac aacggcacca tcaggatggc tcccagcacc accccgacgc
     4741 cgagcccaac cgcctgatcg cgcatccagg ggccgaaggt ctggttcagc agtccatatt
     4801 tgtgctcgcg gaagtagccc tcatacacgc ccatagggaa cgtgagcacc gacacaacga
     4861 cgatgaactg cacccagtaa agtgccgttt gcagcggacg gaatcgggta atgcgttcgg
     4921 ccaggttgcg catgcgcgcc gaccacccga agcgcagcag caaccacatc acaacgaccg
     4981 tagaaagtaa gtcccacagc tgcagccaat aaccgccctc gaaataagca tcggaacggg
     5041 cgcgttgcgc cggcggcatc ttggccagat aggtgtcgac agctgccttc acatcgaatg
     5101 ccggcccact ctggtttgca ggaaccgcag tggacgaagc accgggagca ctcgtttgcc
     5161 cgaatacaag aacactagac agcagaatcg tcagcacaac caattggcct gcccgaacca
     5221 ttacgcctcc ttacgatttt ccggacgagt atacgctgtt gggttctcca ctcgaaccgg
     5281 ggggcccgcc gagcgccggt cttgcgctcg gtggggtgga agccggggtc gcttgagtgg
     5341 gtctttgacc ctcattacgt tccggtgacc gcccatccaa gtcgtcgttg tttgcttgat
     5401 gttttcgaaa ctcgaaactt gaaacttgaa actgaagatt gccgaggtcc cattctggac
     5461 acgcccggtt actttcgttt tctcgccctg gcgattcttg gtgacatcct tcaccaatcg
     5521 ctctatgagt aagactcgac tgttttgctc acatgacggg ctcgagctgg aaccaatgcg
     5581 accaacccgc taagttcttt gacatcagga ttttgcctgt aagttgctct agcgccggat
     5641 tttgcgggaa aattctcgct aagctcatga ttccacggaa tttcagccac atgaattccg
     5701 ctcgcgtgcg ccctgcacca tcgggtgaat gcacgaacat gcgggcataa ctccttacgg
     5761 ctgatatgga tagctataaa ccatttgcaa tcatgcgctt gcgacctatt ttctcccaag
     5821 ttgataaaaa caaacgatgc gttaaaacgt aattttggct aatttcctgt aacggaacgg
     5881 gaagtggatg gcgatgcacc tcaagcggaa cgagaccagt actttcgacg tatcatcact
     5941 cagaggccat catgttcacc tttctcttgt ggatcatcgt gcttatcttc tgctggccgc
     6001 tggcactcgt ggcccttgtg ctgtatccca tcgtgtggct gctgctgttg cccttccgca
     6061 ttctcggcat cgcggttggg ggcgttctcg aactggtgaa ggcgattatt ctgctgccgg
     6121 cgaggatact gcgcggacct agccgggctt tctaacaccc gttgctcaac gacgacagcc
     6181 gcatgctcat gcggctgtcc gcctcggaac acagtaaaat agattcttta gcggctggcg
     6241 tgcgttccgc cctctcctgc ccgcttgatc cgaggacacc aggaccccta tgaacaccac
     6301 caaccacgcg atggagacac cagccacatc cccatccgag gcaccgaaag cctcaaattt
     6361 cattcgcgac atcattcttg ccgacctcga atcgaacaag tttggcggac gcgtacaaac
     6421 ccgctttcca cctgagccca acggctacct gcacctcgga catgccaagt ccatctacct
     6481 gaatttcggg ctggcatcgg aatttggtgg cggaaccaat ctgcgcttcg acgacaccaa
     6541 tcccgaaaaa gaagagcagg aatacgtcga ctcgatcgtt cgcgacgtgc gctggctggg
     6601 ctacgaatgg gcgggacttt attacgcctc tgactacttc gaccagctct atgcctgggc
     6661 gctgcaattg atcaaggtgg gcaaggccta cgtttgcgat ttgaacgccg aggaagtgcg
     6721 gcagtatcgc ggcaccctga ccgagcccgg caaggaaagc ccataccgcg accgcacggt
     6781 ggacgagaac ctcgacctgt tcgagcgcat gcacgccggc gaatttgaag acggctcgcg
     6841 cacgctgcgc gccaagatcg acatggcttc gcccaacctg aacatgcgcg atccggtgat
     6901 gtatcgaatc ctgcacgccg cccacaaccg cacgggcgac aaatggtgca tctatcccat
     6961 gtacgactac gcgcatggcc agtcggactc gatggagaag atcacccact ccatctgcac
     7021 ccttgaattt gaagaccacc gcccgctcta caactggttc atcgagcaac tcggcatctt
     7081 tccctcgcag cagattgagt tcgacaggct gaatgtgacc tacaccctgc tcagtaagcg
     7141 caagctactg caacttgtgc aggagggtcg agtgcgcggc tgggacgatc cccgcatgcc
     7201 gacgctgtca ggcattcgac gccgcggctg cacgcctgaa gcgctacgca acttctgcgc
     7261 cgccatcgga gcatcgaaga ccaacggcac catcgagctg gccatgctcg cgcactatgt
     7321 ccgcgaagac ctgaaccggc gatcgttgcg ggccatggcc gtgcttcgtc cgctgaaggt
     7381 cgtgatcgac aactaccctg aaggccagtc cgaagagttg cagctcgtca acaatcagga
     7441 agatcccagt gccgggacac gcgccgtgcc cttttcgcaa gtgctctaca tcgagcagga
     7501 cgacttccgt gaagatccgc cgaagaaatt cttccgcctg tcaccgggcc gcgaagtccg
     7561 tttgcgcggc ggctacttca tcaagtgcac cggagtggtg aaagacgcga ctggagcgat
     7621 caccgaagtg cactgcacct acgatcccga aacccgtagc ggtaacgctc ccgatggacg
     7681 caaggtcaaa gccaccatcc attgggtctc gggcgaacac tctatcgacg cggaagtccg
     7741 catctacgag aatctatttg ttaaggaaga ccctagcgaa gttgaggggg acaacacgtt
     7801 tctttccaac cttaatccca actcgtttga aatcctgacc gactgcaagc tcgaaccctc
     7861 actcgcgagt gcgaagccgg gagaacgcta ccagttcgag cgaatgggat atttttgcgt
     7921 cgatccagac tcgacagccg agcggttggt gttcaaccgc accgtagcat tgcgtgacac
     7981 ctgggcgaaa atcgagcatc gcgacaaggc gagcacagcg taattggaca cgccaaccgc
     8041 gaacccgcct tagttgtgtt tcacgaactc ggcgttcttc tggttggtca gtgagttata
     8101 cagcgtctcg atgaagacgt cgctgttgat gaagtcgccg gaatacttct tgtcccaggc
     8161 agcgagaatc ttttctttct tcatttgatc gacgctcttt cccgacttga tcgccgcctg
     8221 cactgctgcg ctggtttcct tcagcatggt gacgtactcc cgaacctcat tcaggttcga
     8281 gatctggccg tgaccgggga tgaccttcac gtccgcgggc agcatggcgg cgaccttctc
     8341 gcaggccgcg atcatgcctt gcacgctgcc accggcggtg acgtcgataa agggatagcc
     8401 gtagcgcaca tagtcatcgc ccatgtgcac cacattggcc ttgggaaaga agatgatggc
     8461 gtcgccatcg gtgtgcccgg cgggaaagtg caaggcgcga atgtcatcgc cattgagatg
     8521 gacggtcaca tcgtgatcga aggtgatgat cggcagcgca cccttggccg cgggctttac
     8581 gtccatcgcg accgatgctc cattaccggc cttgccgcca ctctccaggc gcttgcggac
     8641 gttgtcctgc gcaatgatag ttgagccgcc cgtggcgaac gcgaggttcc cgccggtgtg
     8701 atcgccgtgg tagtgcgtgt tgatgacgaa gcgcactggc ttatcggtaa cgccaatggc
     8761 tttcagagcc gcggcgattt tgtcggccag cggagcgtac tcgtcatcga cgatgacgat
     8821 gccgtcttcg cccacggaag cggcaatgtt tccgcctgca ccttccagca tgtagatgtt
     8881 gccggaaacc ttggtgacct tgatctggac cttgctgaag tcctggtcct gggccacgca
     8941 cactgcggcg acgcatagca agacaatgaa cgcgagtctg cgcataaacg tcctctcccc
     9001 cgtgaagtcg caggattata gcattgcgac tagaggtcga ggcatacgca acgccgcgtg
     9061 gctattcata ttgcgacatg gtcagcgtct tgccgtctgc actgcagacc catagtttac
     9121 cgccaacgac agcgccgcag gtgagcggcg catccgactg cagggccgcg gatttcacgc
     9181 ttgcaatgtc ccatagcatt gcatgcggcg gcttctcgcc ggcatcatca aaatagacac
     9241 ttgcagcgtg ctgtttgtcg cccagaacat tgatggcggc gaccgatgat cccgtctcgg
     9301 gaggaaactg gcggagttgc ttggcggtgg cggcatcgag caccgtgata cgggcgtcgg
     9361 cccctcccgc aagcacgtgc ttgccgtccg gcgtaaacgt cagcgcgaaa gtttccagca
     9421 ggaaatcgct gttctcccgc ctctttttcc atgtcgcggt atcgtaccag cgaagcaccg
     9481 tatcatcgcc ggcggcgaca agcagtttgc ggtcaggact cacggcgcac gcaatcaaac
     9541 cgccgaactc gaaaggcaaa gtcgcgcgga gcgtcggagt attcgcgatt tcccacacct
     9601 ggccgggact gtccaccggt ccgacaacga gggactttgc atcggggaaa aaatacatca
     9661 tgtcaattcg gcgcgaactc gctttccagc gtttggaaga agtagcgccc gggctggaga
     9721 acagttccac cgtgccatcg tcaaatccga cggccagcca actcccgtcc tgcgaataga
     9781 cgacgctgtt ggcgtgctga tcggccgaaa acacgtgctg ttcggttccc tcgggaacgt
     9841 tgaccacgcg aacgctgctg tccttacagt gcacaaccag ttgggtgccc gtgggcgaga
     9901 ggtcctgata ccgggactcg cagggcaatt gcactttcgt gatcggtttg gaggtgccca
     9961 tggccgatgc acaaagaacg agaaacgcgg ctgtgaccat ttggcgcatc acagcgtctc
    10021 catcacgagg tcgaagttga cagcggcgat gatttgcttg ggatctccct tgatcaccac
    10081 cggacgcacc aactcgcggc tagtaatgag cggatcgcgg gtgaagttct tttcgggatt
    10141 gccatcgaaa accgggtcgg tggcaaagta tagctgcgtg atcagcggct tatgtccagg
    10201 cgcggtgatg aggtaatgca cgtgttggca aacgcggccg gggtagtgcc caggcatgac
    10261 cgactcgatc gcgtagctcc cctttgctcc aggttcgagt acggcgcggt agcggtagcc
    10321 ctcgacatcg tagtggccgt cattatcggt ctgccagatc tcgacctttg cattgggaat
    10381 aatttcaccg cgagtgttgt acaccccacc ggcgatggtc agcggcatgc cggaatcgcc
    10441 ggcggcgcgc atcacggaag tgtgcggagc ttcgcgtttg tagaatgggc ctagttcgca
    10501 gaacggagta ggctgtttct tcttctcggc ttcgtcccag tcggtggcga gggctgatag
    10561 cggcagtgac gtagcgagcg tgatcgcgcc gaaagcggcg cagcgttcca agagttcgcg
    10621 tcgagtaaag tgcattctct cccctgatac tggggagtga ctataaacga gttcgcctga
    10681 aatcgcctaa ggaaaatcgc ctgcgactat cgtgcggcgg ccgctgcgac tttcgagcct
    10741 ctgcgcagtt tcgccgcttt caccgtatta ctcatcaaca tggcgatggt cagcgggccc
    10801 actccgccgg gtacaggagt gagcgctccc gcgacttcgg cgaccttggg atgcacgtcg
    10861 ccgatcagcg tcgatccgtt ctttttgaag ctctgttcgc gcttggcatc accggcaaag
    10921 aatttctgaa actcaacggg atcggtaatc ttgttcatgc caacgtcgat cacagttgca
    10981 cccggcttca cgtactccgg ggtgatcatg ccagtgcgtc cgatcgccgc gaccaaaatg
    11041 tcggcacgac ggcatacacc aggcaagtcg cgcgtcttgg aatggcatac ggtgaccgta
    11101 gcgttgccat tcagcagaag catggccaca ggcttgccga caatgtcgct gcgtcccacg
    11161 accacggctt ccgccccggc aatagcaatg ttactgcgct tgaggatctc gatgacgccc
    11221 gccggtgtac acggcaccaa gcccggacgc tgggtggaga gatacccaac gttcatagga
    11281 tggaatccat ccacatcttt gtccggtgaa accgccagca gcaccttctt cgcgtccacg
    11341 tgcgccggca gcggcaattg caccagaatg ccgtcgatat cgtcacggcg attgaggtcg
    11401 accaccagcg ccagcagttc ttcggtagta gacgattccg gcggcgtgat cttctcgctc
    11461 atgatgccta gttcttcgca ggccttcacc ttgctgcgaa cgtaaatttc cgaggcggga
    11521 ttgtggccta ccagcaccac ggcaaggccg ggacggagtc cgtccgccgt aagttgcttg
    11581 acctcctcgc caacttcggc tcggatctgc gccgcgattt tggtaccgtc gagaatgatt
    11641 gctgccacgg gccctccggc aagagaatga gttactagtt tagcgcggag gagggaagca
    11701 cttagcaata agcaatatag tgataagtaa taagcaaaag ctttcaatta caggcaaacg
    11761 cttaatcgtg acgagttacg gatacgccac gcgtgcccag cgcgatccgc acgtcggcgg
    11821 ctccggcatc ctcggcactt aaagctgatt acttattgct aattgctgat agctttcttg
    11881 ctacaatcgg cacgtcatga tttccccaga actcctcgaa attctcgcct gtcccgtctg
    11941 ccgcaagcct cttgtgctgc gcggggaaga ctgcctgaaa tgcaacgagt gcaatcgggt
    12001 ttacccaatt cggaacggga ttcccatttt gctcgagcat gaggcgacca tcgagtcgct
    12061 gtaagactcg ggactcggtg aaatggcgcg gcgcgttgcc caatcttaat ctgcgcacct
    12121 ttgcctgact ggacccccag tcatggtggc agattcccca gcctgcatga ccacggtgga
    12181 tttggcccat ccagtttcag cgttttctga ttgttagcgc ggcctcttcc ggagacggtt
    12241 gttatggtct gaccgagtcg aggtgccgtt cgagaccctc accagcaact aatctcaggc
    12301 ccgagtgttg aaatcaacaa aagttgtcga agtgtaaggg tgagatggag gttcggcacg
    12361 tcaattgctg caactttctt gtaagctcgt ccgtctaatg agaaaagccc gtaagggtcg
    12421 gaggtgagcg cgatggcaga aaagttttcc gtccccgagt tggcggcgct gcggagcgaa
    12481 ctgctgttgg gattcgactc caaagacgcc gccgaactgg tccagctctt cctcgctgga
    12541 cacggttatg gtgtatcccc cgaggctgcc cgctatgcgg taagccgggt gggactcatg
    12601 ggcaacacgc tggaggtgtt ccagcgggaa ctggagagca ttgccctggc aatgtagctg
    12661 ggccggctcc ccgagtgctc gccgtaaatt cggcgaagcg gtgacaacaa tccgagaaac
    12721 ggtttcgcgg taatggcctg cgcctgacgt caaaaactgg acgccatgtc gcgatcctga
    12781 agcgaaaaga tgagcccggg caaaacttcc cgggctcgtt gcttgtatgg ggactcccga
    12841 gcgaatccaa accacttcca aacgttgctt cattcggggc gtgcgaagcc gccgttgctc
    12901 cgtatgacgg taaacgacat tcggagtcag gctaagcggt gcccaggcgc ttcagcacag
    12961 attctcggaa gccgcggctt agcgtcaact tcgtgccgtc cctcagcagg atcgcgtaat
    13021 ccccgtacag cgacggcttc agcgtcttga tgcggtcgac gttcaccagc gttgagcgat
    13081 gcacgcgcag gaattggcgc gctcccagac ggttttccaa ggcggtaatc gtttcacgca
    13141 ggatgtgagt gacgttcccc acgtgcaggc acgcgtagtt tcccgccgac tcgacccagt
    13201 cgatttcatt cggcttgagg cagacaatct ccccgtcggc ttttaccacg atgcgatcgg
    13261 gcgcgtgccg gcgggagtcg aggccgccca gcatctcgag aacgcgcttc tgaaacatag
    13321 cctcggaagg atgcatcaat tgcttgcgcg catggtcgag cgcttcgccc aaccgttcct
    13381 ggttgactgg cttcaacagg tagtcgaggg catgcacctc gaaagcgcgc atggcgtaac
    13441 ggtcgtatgc ggtcacgaag atcgtcagcg gcagaagggc ttgcttctcc agttctttca
    13501 ccacgccaaa gccatcaact tcaggcatct ggacgtcgag aaagacgagg tccggcatct
    13561 cgcgctggat aagggcaatg gcagacttgc catcgccgca ctcggccacg atgtccaggt
    13621 ccggctcttt ctgcagcaag ccacgcaaac gttcgcgagc aagcgcttca tcgtcgctaa
    13681 gcaacactcg aattgacatt tgggttcgtg acctcctcgt ttttagatcg atagggaacg
    13741 cggatcttca ccgtgcatcc gccgccaggc gcatcttcca gcaccatggc atggttttcg
    13801 ttgtaaagct gttgtaagcg gccgcgggta ttgcgcagtc ccacaccctc gggcggcagg
    13861 actccccggc cccgcatcaa tcctgtgccg tcgtcgcgaa tttccatcca cacatcatcg
    13921 ttatcacgcc acgtcgcaat gcgaatcaat ccgggcgcgc gccgcggtcc gatgccgtga
    13981 cggatcgcgt tctccaccaa cggctgcaga atcaggttgg gcacaagcgt gtccagggtg
    14041 cgcgggtcga tgtcggtctc gacccgaagg cgatcgtgga aacgggtctg ctgaatgtca
    14101 atgtatttct gcaggaacat gagttcctga tgaagcggaa cttcatgcac acccactccg
    14161 tcgagc
//
LOCUS       DASRKK010000622         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_251681, whole genome shotgun sequence.
ACCESSION   DASRKK010000622 DASRKK010000000
VERSION     DASRKK010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251681"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFC15_15190"
     CDS             <1..567
                     /locus_tag="VFC15_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11550.1"
                     /translation="QGYSSEGAEIVKAIDAITQQCSQARFFYIKGVLQQNTNLEVESD
                     KTKVIGFLDSLGFDPILTASLVKAEDLYRASSDAFDLKSCLGHIRSFYEHLNIDAGQA
                     IAKDLGTTVVDEWDPTITFLKNKSFLSAQQDKFARGLYALLSDEGVHPLIAEREFARL
                     LRNVVIEYGLMFLTMLQKKGVTIQASNP"
     gene            complement(985..1962)
                     /locus_tag="VFC15_15195"
     CDS             complement(985..1962)
                     /locus_tag="VFC15_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HZM11551.1"
                     /translation="MARPPFPKTLRQFQTDFATEEACRQYLTACRWPDGFSCPRCGHE
                     KAYELVKQGRHQCVKCRHQVSLTSGTVLHRTKIPLTHWFWAAYLMTTDKRGVSALLLQ
                     QQLGLSSYETAWMMLHKLRRAMVNAARDPLRGEIEVDDTWVGGEQAGLKGSRQLKDRR
                     AALVLVAVEKRGHASGRARMNVIPDFKASTIIPVLTRNVAPGSTIYTDGLKSFAGLVE
                     AGFEHVTRIQPLRSELRKGAKSAVPFADRAVGNLQQWLIGTYHGVSKAQLQVYLDEFV
                     FRHNRRKTPAAAFQTLLGLGTGRKSTEYDQIRGARDLNHNRLWVAEATR"
     gene            2024..>2383
                     /locus_tag="VFC15_15200"
     CDS             2024..>2383
                     /locus_tag="VFC15_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11552.1"
                     /translation="MIGLKAVNCNTGDVLAEAQEQAPGKEEVLKALDNAAVSLRGKLG
                     ESLRTVEKYATPLAQATTSSLEALQAYSLGQKTARAKGDTAALPFYTRAAELDPNFAM
                     AYVRLAGVYATLNELGRG"
BASE COUNT          514 a          661 c          705 g          503 t
ORIGIN      
        1 cagggttata gcagcgaagg cgcggaaatc gtaaaggcca tcgacgccat cactcagcaa
       61 tgcagccaag cccgattttt ttacattaag ggcgtgctcc agcaaaatac gaatctggaa
      121 gtcgaaagcg acaaaaccaa agtgattggt ttcctcgata gccttgggtt cgatcccatt
      181 ctgaccgctt ctctcgtcaa agcggaggat ctctaccgcg cgtcgtccga cgccttcgat
      241 ctgaagagct gtcttggaca catccgcagc ttttatgaac atctgaatat cgatgccggg
      301 caagctatcg caaaggactt gggaacgacg gtcgttgacg agtgggaccc gaccatcacg
      361 tttctgaaga acaaatcatt cctgtcagca cagcaggata agttcgcccg gggcctctac
      421 gctttgctga gcgacgaagg tgttcatccg cttatcgcgg agcgtgagtt cgcgcggctg
      481 cttcggaacg tggtcatcga atatgggctg atgttcctga cgatgcttca gaagaaaggt
      541 gtcactatcc aggcgtcgaa tccgtgaaaa acaggggtag gcagttcgta tttgcttggg
      601 cgaccagcca gcctttagaa aatgcctagt aggcaatccg atgccgccgg gtctgtcgcg
      661 gccgggccac ttgagccagc gttgtggcgc agctctgaaa acaacacgat cgctatcgca
      721 aagatttggc gtatgcccgt gagatgtcgt ggccggtaga agaggttcca tttcacgttc
      781 gcccgaccgt ttggtgaatc gccacaacct attaattgcg gtacagcctc agttcacgat
      841 ttgcgcggcc ttaagccgag cgttttcagc gcgggcggga ggggttggaa tcggggattt
      901 tggtacagcc gtacaaaccc ttcgcacaac ttcggggacg cccgtcattg gaccagaaac
      961 gctgcttggt tagccgactt ctgattatct ggttgcttca gcaacccaca atcggttgtg
     1021 gttgaggtct ctagctcctc gaatctgatc atactcggtt gatttgcggc cggttccgag
     1081 accaagaagg gtctgaaacg cagcagccgg cgtcttccgg cgattgtgcc gaaagacgaa
     1141 ttcatcgagg tatacctgga gttgggcctt acttacgccg tggtaggtgc cgatgagcca
     1201 ctgctggagg ttgccaacgg ctcgatcggc aaatggaacg gcggacttcg caccttttcg
     1261 tagctccgag cggagcggct ggatgcgagt gacatgttcg aagccagcct caaccagacc
     1321 tgcgaaactt ttgagaccgt ccgtatagat ggtggagccc ggtgcaacgt tccgggtaag
     1381 taccgggatt atcgtgctgg ctttgaagtc gggaatcacg ttcatgcgag cccgaccgga
     1441 cgcgtgacca cgcttttcca cggccaccaa aacgagagcc gctcgtcgat ccttgagttg
     1501 ccggctccct ttcagcccgg cttgctcgcc gccaacccac gtgtcatcaa cctctatctc
     1561 gccgcgcaag gggtcccgag ccgcattcac catcgcccgg cggagcttgt gaagcatcat
     1621 ccacgcggtc tcgtaactgg aaagaccgag ttgctgctgt aaaagcaggg cggacacgcc
     1681 acgcttgtca gtggtcatca gatacgccgc ccagaaccag tgggtcagtg gaatcttggt
     1741 ccggtgaagg actgtcccgg atgtcaacga aacctggtgc cggcatttga cgcattggtg
     1801 acgcccttgc ttcaccaatt catacgcctt ttcgtgcccg catcgcggac aactgaaccc
     1861 atccggccaa cggcacgccg tcagatactg ccggcaagcc tcctctgtgg cgaagtcggt
     1921 ttgaaactgg cgaagcgtct tggggaacgg cggtcgagcc acccttatac actacccatt
     1981 ggggtttgct gaagcaaccg gataatcaga aggcagcaag tacgtgattg gcctgaaggc
     2041 ggtgaactgc aacacgggtg acgtgctggc ggaggcgcag gagcaggcgc cgggcaagga
     2101 agaggtcctc aaggctcttg ataatgcggc ggtcagtttg cgcggaaagc tgggcgagtc
     2161 gcttcgcacg gtggagaaat acgctacgcc gctggcgcaa gcgaccactt catcactgga
     2221 ggcgctgcaa gcctatagcc tggggcaaaa gacagcccgt gcgaaagggg acacggccgc
     2281 gctgcccttc tacacgcggg cggcggaact tgatcccaat tttgccatgg cctacgttcg
     2341 tttggcgggg gtctacgcca ccctcaacga actcgggcgt ggg
//
LOCUS       DASRKK010000623         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1426293, whole genome shotgun sequence.
ACCESSION   DASRKK010000623 DASRKK010000000
VERSION     DASRKK010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426293"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..593
                     /locus_tag="VFC15_15205"
     CDS             306..593
                     /locus_tag="VFC15_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11553.1"
                     /translation="MGFPTKVQLIKRKASEQWYINFPSAVAQAMEFARGETVEWLVED
                     KSLLALRRPQAPPPVLKKTLRSSSTASSNSGASASPAAPNSESTTGRKPSR"
     gene            608..1951
                     /locus_tag="VFC15_15210"
     CDS             608..1951
                     /locus_tag="VFC15_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11554.1"
                     /translation="MGRRTVTGLLSTCGLQFEDWSAAYRLFSKSRVSTGNLFDGVRRS
                     VAELVPEQTPLCLALDDSLFRKTGMKTHGVAWRRDPLGPKFQVNFIRAQRFLQFSAAV
                     ASPEHAQAIRMVPVDFLHCPTPPKPGKQATPEQLSTYREACRQANLGTLAIDRLKAIH
                     ASLPPNSNGQPRAVRLLVDGHYTNRTVLRDLPPDTTLIGRVRKDAKLCYPAAVRPDGK
                     LGRRALYGAPAPTPEQLRTDESVAWETVSVFAAGAQHQCRVKTLSGLLWRASGAQRRL
                     KLVVIAPLHYHLRVGSKLLYRQPAFLLCTDPDLDTQQIVQNYVWRWDIEVNFHEEKSL
                     LGIGQAQVRTPASTQHLPALLIASYSMLLLASIRSLSQAEPPLLLPRPKWCARSKPIR
                     TSTQRIVHQLQAEIWGRGLGLNPQSFSGFPSHTTPIQKPQKLLPSLADAVLYCNA"
BASE COUNT          463 a          811 c          687 g          467 t
ORIGIN      
        1 acacagtgtc atcaaaaagc agggcgaagc cagccgattc gggagaaagc ggcgaagcgg
       61 aaccctgccc tgagtcgatg gaatcgaacc cacgagcagc cgtactgtct tcgttcgcca
      121 tgtggggcgg cgctccagcc ccattttcag aaatagggcg ggccctccag ggcccgctct
      181 ggcgtttggc tcttaactag ataaacacgc gacggggcag gggtagctac gacatttctg
      241 cccaattttg ttctttctcc tggcattttt tggtatctag ttgtatgact agagtagagg
      301 tgcccatggg tttccccacc aaagtccagc tcatcaaacg caaagccagc gagcagtggt
      361 acatcaattt cccctccgct gtcgcccaag ccatggagtt cgctcgcggc gagaccgtcg
      421 agtggctcgt cgaagacaag tccctgctcg cgctccgccg gccccaggcg ccgcccccgg
      481 ttctaaaaaa aactctgcgc tcctcctcga cggcttccag caactctggc gcgagtgcaa
      541 gccccgctgc tcccaacagc gagtcgacga ccgggcgcaa gccctcgcgc tgagttcgct
      601 gctgtgtctg ggacgcagaa ccgtcaccgg cctgctgtcc acttgtggcc tgcagtttga
      661 ggattggtcg gcggcttacc ggctgttttc caagagtcgc gtttccaccg ggaatctgtt
      721 cgacggcgtg cgccgcagcg tcgccgaatt ggtgcccgag cagaccccgc tgtgcctcgc
      781 tctggacgat tccctgttcc gcaagacggg catgaagacg catggcgttg cctggcggcg
      841 cgatccgctg ggtccgaaat tccaggtcaa cttcatccgc gcccaacgct tcctgcaatt
      901 ctcggcagcc gtagccagcc ccgagcacgc gcaagcgatc cgcatggtgc cggtcgactt
      961 tctgcactgc cccacgccgc ccaaaccagg caagcaggcc acgccggagc aactgtccac
     1021 ctatcgcgag gcgtgtcgcc aggccaacct cggcacgctg gccatcgatc gcctgaaggc
     1081 cattcacgcc agcctgccgc ccaactccaa cgggcaaccg cgggccgtgc ggctactggt
     1141 cgacggccac tacaccaacc gcaccgtgct gcgcgatttg ccgcccgata ccaccctgat
     1201 tggccgcgtc cgcaaggacg ccaaactctg ctatccggcc gcggtcagac ccgacggcaa
     1261 gctcggtcgg cgcgccttgt acggcgcacc cgcacccacg cccgagcaac tgcgcaccga
     1321 cgagtcggtg gcctgggaaa cggtatcggt gtttgccgcg ggcgcccagc accagtgccg
     1381 cgtcaagacg ctcagcggac ttctctggcg cgcttccggc gcccagcgtc gactgaaact
     1441 ggtggtgatc gccccgctgc actatcacct gcgtgttggc tcgaaactgc tctaccgcca
     1501 gccggccttt ctgctctgca ccgatccaga tttggacaca caacagatcg tgcagaacta
     1561 tgtctggcgc tgggatattg aagtgaactt ccacgaggag aagtcgctgc tcggcatcgg
     1621 gcaggcgcag gtgagaacgc ccgcctctac gcagcacctg cccgctctgc tcatcgcctc
     1681 ctactccatg ctgttgctgg cctcgatccg cagcctcagc caagccgaac cgccgctgct
     1741 gttaccccga ccgaaatggt gcgcccgctc caaacccatt cgcacttcga cacaacggat
     1801 cgtgcaccaa ctccaggccg agatctgggg ccgcggcttg ggcctgaacc cgcagagttt
     1861 ctccggcttc ccgtcacaca ccacccccat ccagaagcct cagaaactcc tgccctccct
     1921 cgccgatgcc gttctgtact gcaatgcgta agcagaatcg tggtcggaaa aggccaatcg
     1981 ccagttgccg gacatccgtc cggcacggta caaatcaagt gaccctgacc gcccggaaat
     2041 ggccggtttt gaagtgacct tataatggcc ggttttgagg tgatccccga gggagcatga
     2101 acaagccaag cgatattacc tatcgcgata ggtcaggcat cggagatatc gtggttttga
     2161 gatcgtagct gacggcttat gaacggtacc ataattgtct ccggaaatgt gaatgcctcg
     2221 aagccgacgc cttccaattg ggcttcgggt ttgattctgg ccatgggtcg tagttcgccg
     2281 agcgcacatc ccggcgtggg gtggaatcct ctcgattgcc ctgcccgttg caggaagacc
     2341 ttggggctga gtctcagcct gacagggctg tgactcacac tatgatttca ataggttcgg
     2401 gaccgggtcc cccgcaattg gcgtagag
//
LOCUS       DASRKK010000624         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1132467, whole genome shotgun sequence.
ACCESSION   DASRKK010000624 DASRKK010000000
VERSION     DASRKK010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132467"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1158)
                     /locus_tag="VFC15_15215"
     CDS             complement(301..1158)
                     /locus_tag="VFC15_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11555.1"
                     /translation="MITMKNWRERMGEDMRLRDLRPRTLEAYLLATRLFLERIGKPPE
                     TLTEADIRGYILHLRDDKRQAPSSINIAICALRFFFNFTLQRDYEVFALLRVNRPRTL
                     PVVLSRAEVKRLLGAVEHPVRRMALTTIYGLGLRLGEGLHLQSSHIDSGRQMVWVRSG
                     KGAKDRRVPLPKPLLHKLRAYWKYERPVSTLPLLFVSSEGKPIHETTLQKTFTAAQLE
                     LNLPRRATIHTLRHSYATHLVEHGISLRTIQDVLGHKSLHTTEVYMHVTVPGTERLQA
                     VLDQLMADL"
     gene            complement(1792..2916)
                     /locus_tag="VFC15_15220"
     CDS             complement(1792..2916)
                     /locus_tag="VFC15_15220"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM11556.1"
                     /translation="MCKNTRYLGLDVHAETITAAIAEGRQKIRSVGQFPNRPEAIRKF
                     IEKLGGPNGLKVCYEAGPTGYALYWQLTKMGVECEVVAPSLIPKKPGERIKTDRRDAE
                     KLAQCYQAGTLTAVWVPDGAHEALRDLVRARGAAKDDESRAKHRLVKYLLHYALRHPD
                     NCRAWTHPWWRWLQALKLEHEAQRETLLAHIAEVLHQGERVSRLDKAIERAIESVSPE
                     KKAVINALQALRGVAQLTAVTIMTEVGTFQRFRKPTELMGYTGLVPSEYSSGGKESKG
                     RITRTGNAHLRHVLGETACHTRHKPWLSNRLKQVLPTLSPGVSEIAWKAQERLHRKFV
                     KMTYQNKQPGKVLTAVARELVGFIWAIGSKVEREALAKAA"
BASE COUNT          639 a          971 c          884 g          763 t
ORIGIN      
        1 caacatggaa gtacggtacg gacagcaaca agtcacgctg atgcataagc cagcgttctg
       61 tcgcatcgtg gccacattgc ggacacgcac gatgccgaca cgaatgatac agcaaatgct
      121 cgtgtccgca ctgcgtgcag cgggcagagt gtccgcccag cgcgggtgta cgacataaag
      181 caatcgcccg aagcgcgcgc acgtggctcg gcagtatggc atcaccgaag cgagccaagt
      241 acccagtcgc atgccgccgt accacatcgg caagccgcag cactgcgcca gctccgtggg
      301 ctacaggtca gccatcaatt ggtccagcac cgcttgcagc cgctcggttc ctggcaccgt
      361 tacgtgcatg tacacctcgg tcgtgtgcaa actcttgtga cccagcacgt cctgaatcgt
      421 gcgcagcgag atgccgtgct cgaccaagtg cgtcgcatag ctgtgtcgaa gcgtgtggat
      481 ggtggcacgc cgcggcagat tcaactcgag ctgtgccgcc gtgaatgtct tctgcagcgt
      541 ggtttcgtga atcggcttgc cttcggatga aacgaacagc aacggcagag tcgaaacagg
      601 acgctcatac ttccaatagg ctcgcagctt gtgcagcagt ggtttgggca gcggcactcg
      661 ccgatcttta gctcctttgc cgctgcgcac ccacaccatc tgccggccac tgtcgatgtg
      721 actcgattgc agatgcagcc cttcgcccaa gcgcagccct agcccataga tggtggtcaa
      781 tgccattcgc cgtacaggat gttcgacagc acccagcagc cgcttgacct ccgcacgact
      841 gagcaccacg ggcagcgtgc gcggtcggtt gacccgaagt agagcaaaga cctcatagtc
      901 gcgttgcagc gtaaagttga aaaagaacct cagcgcacag atggcgatat tgatacttga
      961 cggggcctgt cgcttgtcat ctctgaggtg cagaatgtac ccacggatat cggcttctgt
     1021 cagagtctcg ggtggtttgc ctattcgctc gagaaatagc cgtgttgcca ataggtaggc
     1081 ctcgagtgta cgcgggcgca agtcccgcag ccgcatgtct tcgcccatac gctcgcgcca
     1141 attcttcatc gtgatcatcg tcttctccgt tgaaacactc ggaggcacca cacctccgag
     1201 tgggcaacat tgaagacccc ttttgccgca gtgcggaact gaacagactg gcactctatt
     1261 accgacgaat ccaggcagtc ttgccgagtt gagctaccgc gcgactagcg cggtttcgtt
     1321 caactcgcgc atgaacttgc cagctttgtt ttctgacagt tcgtggctgc cgggcgccgt
     1381 ggctcgtcgg agtcgcatca attttacggc gcccggcagt cttccaactt gatatgctat
     1441 ggtgtttctg ttgggctgca agttatgcgc aagccgttga tatggaacga cccgtcaagt
     1501 cttgacgcga cgtcatttca cttttcttcg ttcgatagtt ttctttcttt ttttagggga
     1561 tgaaacagag agtgaacgac cgaaagcgag cttagtcgac ggtcatcaag ctgatattcg
     1621 agggttggcc cgaagacgta cctcattgtc tttcatgcgt cgggagctgc ctcggactag
     1681 gcaacgcggg ttggttaccg cacaacggct tcgaggattc tcctttccgt gccactctcg
     1741 gtcgctcacg ctctgtttca tcgttggtgt tgctgggtgt gattggtttg cttacgccgc
     1801 tttggcgagt gcctcccgct ccactttgga gcctattgcc cagatgaaac cgacgagctc
     1861 acgagccacg gcggtcagca ctttccccgg ttgcttattt tggtaggtca tcttgacaaa
     1921 cttgcgatgc agccgctcct gagccttcca ggcaatctca gagacaccgg gtgacagcgt
     1981 tggcaacact tgcttcaatc gattgctcag ccatggcttg tggcgggtat gacaggcggt
     2041 ttcgccgagc acatgtcgaa ggtgcgcgtt gcccgtgcgt gtgatgcgcc ctttggattc
     2101 cttgccgcca ctcgagtact ctgacggaac cagccctgtg taccccatca gctccgttgg
     2161 tttgcggaat ctctgaaacg tacccacttc ggtcatgata gtaacggccg tcaactgtgc
     2221 aactccgcgc agcgcttgca gggcattgat gactgccttc ttctcgggcg atacggattc
     2281 gatggctcgc tcaattgcct tgtccaaacg actcacgcgt tctccttgat gcaatacctc
     2341 tgcgatgtgc gctagcagcg tttctcgttg cgcctcgtgc tctaatttca gtgcctgcag
     2401 ccatcgccac caaggatgcg tccaagcgcg gcaattgtct ggatgccgca aagcgtaatg
     2461 caacagatac ttaaccaatc gatgcttggc acgactctca tcatcctttg ccgcgcctcg
     2521 cgcacgaacc agatcacgca gcgcttcgtg cgcgccatcg ggaacccaca ctgcagtcaa
     2581 tgtgcccgcc tggtagcact gggcgagctt ctcggcgtcg cgacgatcgg tcttgatccg
     2641 ttcgccgggc tttttcggaa tcagcgatgg cgcgacgact tcgcattcaa cgcccatctt
     2701 cgtcagctgc cagtacagtg cgtagcccgt tggccctgcc tcgtaacaga ccttcagccc
     2761 attggggcca cccagctttt caataaactt cctgatcgct tcgggccgat taggaaattg
     2821 cccaacactt cggattttct gccgtccctc ggcgatcgcc gccgtgatcg tttctgcatg
     2881 tacatccaaa ccgagatatc gtgtattctt gcacatgacc gactcctcct ttgtggctct
     2941 gtgtctcagt ttcttcaccg agaccgagca taacccacgc ctagggcgcg agtcggtcgt
     3001 tccatcatga ctaggcaggg tggctctctt cgaggcagcg atagcttcaa tcaggtcctt
     3061 ggttcggcgg ttcgggacct ctagctcgaa ctgatcgaca acaccgagca gctcatcacg
     3121 ggtgagcacg ggcccggttt tccgacaacc cggaaagact acgcggttgc gatttctttt
     3181 cagcacccac ataggaccaa aatattgccg tcgaacgtgg gtgtttctgg atcgcaagta
     3241 tagtcgcaga tctccgg
//
LOCUS       DASRKK010000625         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_937180, whole genome shotgun sequence.
ACCESSION   DASRKK010000625 DASRKK010000000
VERSION     DASRKK010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937180"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1130)
                     /locus_tag="VFC15_15225"
     CDS             complement(132..1130)
                     /locus_tag="VFC15_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11557.1"
                     /translation="MNSTPTLPALLESYFTQRLMAQRQASPHTIASYRDTFRLLLNFA
                     QKHYGKSPSSLNLTDLDAPLISKFLDALEKQRGTSISSRNVRLTAIRSFFRYTAYEEP
                     AHSAHIQRVLAIQNKRQPRPMISFLVREEIEALLAAPDQHTWNGRRDHAMLRLAVQTG
                     LRLSEVTSLGREALVLGTGAHVRCHGKGRKTRCTPLTKQTAGVLKAWSKEPARGTANA
                     LFPNVHGGRLSSDAVQHLLAKYLALAQQRCPTLKQKRVTFHSLRHTSAMELLAAGVDT
                     SVIALWLGHESVETTQIYLHAHLALKEAALAKTRPLTGRQARFRPGDKLLEFLTAL"
     gene            complement(1127..2059)
                     /locus_tag="VFC15_15230"
     CDS             complement(1127..2059)
                     /locus_tag="VFC15_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11558.1"
                     /translation="MKSLQQAAQDYIRMRRHLGFKMRHEERRLRRFVSFMEQERSPYI
                     TTKLALKWATEPANAQRATWAERLMCVRVFARYCSSADPRTEVPPLGILPFRPQRATP
                     YLYSEEEIRRLMEAARCLAPSGGLRGLTYHCLLGLLTVTGLRISEALALTCEDVDLRN
                     GLLTIRGAKFGKTRLVPLHPSACRALSRYAQQRDELLDLPRAANFLLCASGRPPEVST
                     VRRVFYQLSRQTGLRGPSDHRGPRLQDFRHRCATEILLKWYQAGEDVERRLPVLSTFL
                     GHGHISETFWYLSGSSELLEQAARRLERRWEVQP"
     gene            complement(2056..>2879)
                     /locus_tag="VFC15_15235"
     CDS             complement(2056..>2879)
                     /locus_tag="VFC15_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11559.1"
                     /translation="DYTRFAHQFLRERFARGSVILSTLSATDVTEFVRRHAHERSARS
                     AQHIVGSLRAFLRYLRYQGTIATDLAACVPKVANWSHSALPRFLQPGQVQQVLDHCDR
                     RSAVGLRNYAILLLLARLGLRACEIVAMALDDIDWKAGHLMVRGKGGQRAQMPLPAEV
                     GRAIAVYLRKGRPSCASRRLFIREHAPRVGFANSAAVSTLVQRALADAGVASPHTGAH
                     VFRHSLATEMLRHGASLGEIGQLLRHAHPDTTQIYAKVDVNALRPLALRWPGGGR"
BASE COUNT          613 a          840 c          845 g          581 t
ORIGIN      
        1 cagaatgccg ctttccggaa aatgccgaat cgcgctcgac actgaaatcg gtagccccgt
       61 gtatacggtg cgggacgata tcctcggctt cacgcgactc ggcattgttt ttccatgctg
      121 cggaggcatc tctatagagc agtcaggaat tcgaggagtt tatcgcctgg ccggaaacga
      181 gcctgcctac cggtcagcgg tctcgttttg gcaagcgctg cttctttcag cgcgaggtgt
      241 gcatgcaagt aaatttgcgt tgtctccacg ctttcgtggc ctaaccacag tgcaatgacc
      301 gaagtgtcga cacccgcggc tagtaattcc atggccgaag tgtgcctcaa ggagtggaag
      361 gtcactcgtt tctgcttcaa cgtcgggcac cgctgctgag ccaaggcgag gtatttcgcc
      421 aggaggtgct gcacggcatc agaactaagc cgccctccat gaacgttggg aaataacgcg
      481 ttggccgttc cccgcgcagg ttccttcgac cacgctttca aaacgccagc ggtttgtttc
      541 gtcaaaggtg tgcaccttgt cttacgtcct ttgccatggc accgtacgtg cgcgccggtg
      601 cccaacacca gagcctctcg accgagactc gttacctccg atagacgtag ccctgtctgc
      661 actgctaatc gcagcatcgc gtgatcgcgg cgcccattcc aggtatgctg atccggtgcc
      721 gcaagcagcg cctcgatttc ctcccggact agaaagctga tcatcgggcg tggctgacgc
      781 ttgttctgga tggccaagac acgctgaata tgcgcagaat gtgccggttc ttcgtacgcg
      841 gtatagcgga agaaagagcg gatggccgtt agccgcacat tgcgactgct gattgaggtg
      901 ccgcgttgct tctccagagc atcgaggaac ttactgatca aaggcgcatc cagatccgta
      961 aggttcaggc tagaaggcga cttgccgtag tgtttttgtg cgaagttcaa aagcaatcgg
     1021 aatgtgtcgc gatacgaggc gatcgtatgg ggactagctt gtcgttgagc catcaaacgt
     1081 tgcgtgaagt agctctccag caaggccggc agggtgggcg tcgagttcat ggctgcacct
     1141 cccaacgacg ctccaatcgt cgggccgcct gctccaagag ctcggacgac cctgacaagt
     1201 accagaatgt ttcgctgata tgtccgtgac ccaaaaatgt ggatagtacc ggcaatcggc
     1261 gctcaacgtc ctcgcccgcc tgataccact tgagcaagat ctctgtcgcg cagcgatggc
     1321 gaaaatcttg caggcgaggc ccacggtgat ccgacggacc ccggagcccc gtttgacgtg
     1381 aaagttgata aaagacacga cgaaccgtgg agacttcggg cgggcggcca ctggcacaga
     1441 gcagaaagtt ggcagcacgc ggcagatcta aaagttcatc ccgctgttgc gcgtagcgcg
     1501 aaagggcacg gcaagccgaa ggatgtaaag gcaccagacg ggttttacca aactttgcgc
     1561 cgcgtatcgt gagcagaccg tttcgcagat caacatcttc gcacgtgagc gcgagcgctt
     1621 cactgatacg caaaccggtg actgtgagga gtccgagcag gcagtggtaa gttaaccccc
     1681 gtaggccgcc acttggcgcc agacatcggg ccgcctccat cagccggcga atttcctcct
     1741 cgctgtatag gtaaggtgtg gcccgttgcg ggcgaaacgg caaaatgccc agcggaggaa
     1801 cctccgtgcg cgggtccgca ctgctgcaat agcgcgcgaa aacacgtaca cacatcagcc
     1861 gttcggccca ggtggcgcgt tgcgcattgg ccggctcggt ggcccacttc agcgccagct
     1921 tggttgtaat gtagggggat ctttcttgtt ccatgaagga gacgaagcgt ctcaaacgcc
     1981 gctcctcatg ccgcatcttg aagccgaggt gccgccgcat ccgaatataa tcctgggccg
     2041 cctgttggag ggatttcatc gcccacctcc cggccagcgt agtgccaacg gacgcaaggc
     2101 gttgacatcg accttcgcgt aaatctgggt agtgtccgga tgggcatgac gcaacagctg
     2161 tccgatctcc ccaagcgagg ctccgtgccg aagcatctcc gtggccagcg agtgtctgaa
     2221 tacgtgggcg ccagtgtgcg gggaggccac gcccgcatcg gctaaggcgc gttgtacgag
     2281 tgttgagaca gcggcagaat ttgcaaaacc tacccgcggc gcatgctcac gaataaatag
     2341 acgtcggctt gcacagctcg gtcgtccttt cctcaggtag acggcgatcg cgcggccgac
     2401 ctcggcaggc agaggcattt gagccctctg tccgcccttg ccgcgaacca tcagatgacc
     2461 ggccttccaa tcgatgtcat cgagtgccat agcaactatt tcgcacgccc gcagacccag
     2521 ccgcgcaagc agcagcagga tcgcatagtt gcgtaggccc acggcactgc gccgatcgca
     2581 gtgatccagc acctgctgta cctgtcctgg ctgcaagaac ctgggtaatg ccgaatgcga
     2641 ccagttcgcc accttgggta cgcaggctgc aaggtcagtc gcaatcgtgc cttggtatcg
     2701 caggtatcgc agaaatgctc gtagcgaacc gacgatgtgt tgcgcacttc gtgcgctgcg
     2761 ctcgtgagcg tgacggcgaa caaattccgt gacatctgtg gctgaaagcg tcgacaatat
     2821 gacagaacca cgcgcaaatc gctcccgaag gaactgatgt gcgaaacgcg tataatcgc
//
LOCUS       DASRKK010000626         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1035651, whole genome shotgun sequence.
ACCESSION   DASRKK010000626 DASRKK010000000
VERSION     DASRKK010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035651"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="murI"
                     /locus_tag="VFC15_15240"
     CDS             <1..937
                     /gene="murI"
                     /locus_tag="VFC15_15240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZM11560.1"
                     /translation="YWPGRKGFAVTLPRIGVFDSGVGGLTVLKAMAEQLPAHYLYFGD
                     TARLPYGTKSAETVARYAIGATRFLEAQNIDLLVIACNTATALAFPLIQEAAHVPVVG
                     VVEPGAAMAAEVSKSRSAIVIATEATVSSHAYRDALRRHGLAAHEKACPLFVPLVEEG
                     WIEHPVTEQVARIYLGEALLQAPEQADVLVLACTHYPLIAPLLRRIAPDHMQIVDSAE
                     STARVVRKMLKDETQVAVESDGSNNQAVILSEARLPLAAAERSRRTPINTKVPPAEHR
                     TFRFYVTDSTGKFKRLAARFLGRQVDNVEHVDLGG"
     gene            1104..2408
                     /locus_tag="VFC15_15245"
     CDS             1104..2408
                     /locus_tag="VFC15_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11561.1"
                     /translation="MRANFRIPLLAAFLSLFASFALAGDQTKPGPGNSNAANLASQSP
                     VVQSAFGFLIDQTGGLQGSKLRNETFDAITNPDTCVRHRAGLRPADRQAILNTLLAQG
                     LVDPNDDGTFPGGLITGVFPPVLNDNGPCPHLPQPFYSAPGSVYHGHHSYPGGLPVHE
                     SNNDVSDAHLADQYRAVYGHTGILGLPTIDGNVLWETTDQIQNRDTAIYLDQDVIVGA
                     PIWHDWAKSIVFQWFSDGTEFQELNFGGNGSTDNYGGSGNSKTGGHHIMSIAEAMARG
                     LSPAFVITQASAHSNPTSGNEYKVVNWLRAAAIMARIDPVAKGYLMIDGNGNYRLPAL
                     RKLGSINLGEVNALAEYELHNLSDADFTFSGPAITGVEAVLQSLASEFGYDPSDASRY
                     NNKYRNPVFSFMTAERLYIVYSQKGSKGVEVELRRLRNLGVI"
BASE COUNT          516 a          787 c          681 g          506 t
ORIGIN      
        1 atattggcca ggtcgtaaag gatttgcagt gaccctgccg cgcattggcg ttttcgactc
       61 gggcgtcggc ggcctcaccg tgctgaaggc catggccgag cagttgcccg cgcattactt
      121 gtacttcggc gatacggctc gcctgcctta tggcaccaag tcggcggaaa ccgtcgcgcg
      181 ttatgcaatc ggagctactc gttttctcga agctcagaat atcgatctgc tggtcattgc
      241 ctgcaacacc gcgaccgcgc ttgcatttcc gctcatccag gaagcggcgc acgtgcccgt
      301 cgtgggagtg gtcgaacccg gcgcagccat ggcagccgaa gtgagcaaga gtcgctcggc
      361 tattgtgatc gccaccgaag ccacggtgag cagccacgct taccgcgacg cgctgcggcg
      421 acatggcctg gcagctcatg agaaggcttg tccgcttttc gtgccgttgg ttgaagaagg
      481 atggatcgag catcccgtga ccgagcaggt cgccaggatc tatctcggcg aggcgttgct
      541 gcaagcgccc gagcaggcag acgttctggt gctcgcctgc acccactatc cgctcatcgc
      601 ccccctgctg cgtcgcatcg cgcccgacca catgcagatt gtggactccg ctgaatcaac
      661 cgcgcgcgtg gtgcgaaaga tgctgaagga cgaaactcag gttgccgtcg aaagcgacgg
      721 gagtaataac caagctgtca tcctgagcga agcgaggctg ccgcttgcgg cagccgaacg
      781 gagtcgaagg acccctatta acacgaaagt tcctcctgcc gagcatcgca ctttccgctt
      841 ctacgtcact gactctaccg gcaagttcaa gcgcctggcc gctcgcttcc tcggccgcca
      901 ggtggacaac gtcgagcatg tggacttggg cggatagggc ggtcgctgct cctctctctt
      961 tcctattaac aaaattgtgg aaggaattcc atcgagattt gatctcacat tgaccgcacc
     1021 ttcataagca gcctctaagt tcataagtgt cgtccgcgcc ggcccaatct cggcgcgatt
     1081 caccgaatca aaggagtctg actatgagag caaactttcg catccccctg cttgccgcat
     1141 tcctctctct ttttgccagc ttcgccctgg caggagacca gacgaaaccc ggccccggca
     1201 attccaacgc agctaacctg gccagccagt ctcccgttgt gcaaagtgcc ttcggattcc
     1261 tgatcgatca aaccggtggc ctgcaaggca gcaagctcag aaacgagact ttcgatgcca
     1321 tcaccaatcc tgatacctgt gtcaggcatc gcgctggact ccggcctgcg gaccggcagg
     1381 cgatcctgaa cacgctgctg gcacaagggc tggtcgatcc caacgacgac ggaacgtttc
     1441 ccggtggctt gatcacgggg gtatttcctc ccgtgctgaa tgacaacggc ccatgccctc
     1501 atctcccaca gccgttttat tctgcacccg gcagcgtgta ccacggacac cattcctatc
     1561 ccggcggact tccggtgcat gaatccaaca acgacgtctc tgacgcgcac cttgccgacc
     1621 agtaccgggc cgtatacggg cacactggca tcttgggctt gcccaccatc gacggcaacg
     1681 tcctgtggga aaccaccgac cagatccaga accgggacac cgccatctat cttgaccagg
     1741 acgtgattgt cggcgcaccc atctggcacg attgggcaaa gtccatcgtt ttccagtggt
     1801 tctccgacgg aaccgaattt caggagctga atttcggggg caatggcagc accgacaatt
     1861 acggtgggag cgggaactcc aaaaccggcg gccatcacat catgagcatt gccgaagcca
     1921 tggcgcgagg tctaagtccc gctttcgtaa ttacgcaagc ctcagcacac tccaacccca
     1981 cctccggcaa tgagtacaag gtggtgaact ggctccgcgc cgctgccatc atggcgcgca
     2041 tcgatccagt ggccaagggc tatttgatga tcgacggcaa cggaaattat cgtctgccgg
     2101 cccttcgcaa gctgggctca atcaaccttg gcgaggtcaa cgcccttgcg gaatacgaac
     2161 tgcacaatct gtcggacgcg gattttacct tctccggtcc agccatcaca ggtgtcgagg
     2221 ccgtcctgca atcgctcgct tccgagttcg gctatgaccc ctcggatgcc tcccgctaca
     2281 acaacaagta ccgcaatccg gtcttcagct tcatgactgc ggagcgactg tacattgtct
     2341 acagccagaa gggctccaag ggagtcgaag tcgaattgag gagactgcgc aatttgggcg
     2401 taatttagac gctttcaacc cttctgaccg ggcaccctgg atcgccgtcc tcccggtcag
     2461 aagggttgca tccgcccaag aaggcggtgc
//
LOCUS       DASRKK010000627         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1427152, whole genome shotgun sequence.
ACCESSION   DASRKK010000627 DASRKK010000000
VERSION     DASRKK010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427152"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..294)
                     /locus_tag="VFC15_15250"
     CDS             complement(136..294)
                     /locus_tag="VFC15_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11562.1"
                     /translation="MTITKSETPKKKTTPDALVDKTDVELTEEKLSKVSGGLILDGIK
                     GESKDHKL"
     gene            complement(395..1660)
                     /locus_tag="VFC15_15255"
     CDS             complement(395..1660)
                     /locus_tag="VFC15_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZM11563.1"
                     /translation="MTDNNVIKLAQPGTFIDSLTEILRSGARALLTQAVEAEVADFLH
                     RHCDLKTDTGRRRVVRHGHLPEREIMTGIGPVGVRQPRIRDREAAAGERIRFNPTILP
                     PYVRRTKSLEVLIPILYLKGISTGDFEEALAALVGKDAPGLSASTIARLKEGWTDEHE
                     HWQKRDLSMKRYVYFWADGIHLEARLEDQAQCILVIIGATPEGKKELIGFTDGMRESA
                     QSWRDLLLDLKRRGLAAAPELAVADGALGFWKALGEVWPTTREQRCWVHKTANVLNKL
                     PKNLHTKAKRALQEIWMAETKKDAIIALDAFAESYGPKYDKAVDCLTKDRDTLLAFYD
                     FPAEHWKHLRTTNPIESTFATVRHRTIRSKGCLSNKTALAMVFKLVEGAQKSWRRLDG
                     HNQLPKLIQGVKFTDGIEAVRQDAQTAAA"
     gene            1778..2260
                     /locus_tag="VFC15_15260"
     CDS             1778..2260
                     /locus_tag="VFC15_15260"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HZM11564.1"
                     /translation="MSMNEQRVHHIFLVSVLLKGAHALIECLGGTILALVSTTSIVNF
                     VNAITLNELIEDPNDFIATHLLALARDFSVQTQHFYAFYLLSHGLVKVLLVVGLLREK
                     LWSYPASLIVLALFVVYQLYRFSYTHGFGLIVLTVFDVIVMGLIWHEYNLMRHHLPTK
                     "
BASE COUNT          564 a          861 c          813 g          675 t
ORIGIN      
        1 tagctgcgtg gccaaatttc ttcgcctcag cagttctagt acgctagctc aagcggaccc
       61 agtgttgagc caagcccgct taaactgcta gcggcctcgc cttcaagcgt ccgcatatgt
      121 attagaggta accaactaca atttgtggtc ttttgattca cctttgattc catccaatat
      181 cagtccccca gaaaccttac taagcttctc ctcggtcagc tcgacgtcgg tcttatcgac
      241 gagcgcgtca ggagtggtct tctttttcgg tgtctctgat ttcgtgatcg tcatgtgtct
      301 tctccaaggc taagggtcaa agcttcgcag tcgcaatact tagtatgtga ccatggcccc
      361 gagagctaac gccgaatctt ggtgacgagc ggggtcaggc ggcagcggtt tgagcatctt
      421 gcctgacggc ctcgatgcca tcggtaaact tcacaccttg gatcagcttt ggcaactggt
      481 tgtggccgtc gagccgacgc cagcttttct gggcgccctc gaccagcttg aacaccatcg
      541 caagcgcggt cttgtttgaa aggcagcctt tcgaacggat cgtccggtgc cgcacggtcg
      601 cgaaggtgct ttcaatcggg tttgttgttc gcaagtgctt ccagtgttcg gcggggaagt
      661 cgtagaacgc gagcaacgtg tcgcgatcct tcgtcaggca atcgacggct ttgtcgtatt
      721 tggggccgta gctctcggcg aaggcatcca aggctatgat ggcatccttc tttgtctcgg
      781 ccatccagat ttcctgcaga gcgcgcttcg ccttcgtatg caggttcttc ggcagcttgt
      841 tcaggacgtt cgccgtctta tgcacccagc agcgctgctc gcgtgtcgtc ggccacacct
      901 cgccaagcgc cttccagaag ccaagcgcgc catcggcgac ggcaagttcc ggcgctgccg
      961 ccaagccgcg ccgcttgaga tcgagcagca gatcgcgcca cgattgtgcg ctctcgcgca
     1021 tgccatccgt aaagccgatc agctccttct tgccctccgg cgtcgcgccg atgatgacga
     1081 gaatgcattg cgcctggtcc tcgagccgag cctccaggtg gatgccatcg gcccagaagt
     1141 agacgtagcg cttcatcgag agatcgcgct tctgccagtg ctcgtgttca tcggtccacc
     1201 cttccttgag gcgagcaatc gtcgatgccg acagaccagg cgcatccttg ccgacaagcg
     1261 cggccagcgc ctcctcgaag tcgccggtcg agatgccctt caggtagagg atcggaatca
     1321 gcacctccag gctcttcgtg cgcctcacgt agggcggcag gatggtcggg ttgaaccgga
     1381 tgcgctcgcc cgcagccgcc tcgcggtcgc gtatccgcgg ctgccgcacg ccaaccggac
     1441 caatgccagt catgatctcg cgctcgggca gatgcccgtg gcgcacgacg cgccggcggc
     1501 ccgtgtcggt cttgagatcg caatgccggt gcagaaagtc cgcgacctcc gcctcgaccg
     1561 cctgcgtcag cagggcgcgg gccccgctgc gtaaaatctc ggtgagcgaa tcgatgaagg
     1621 tccctggctg cgcgagcttg atgacgttat tgtcggtcat ggcgtatcac tccttcagtg
     1681 gagaagtgga ggcgtcgaac acccccacga tacgccgcct taccccttca tgccgtcacc
     1741 aacttccgcg catagctctt cctcaattga tcttggtatg agcatgaacg aacagcgagt
     1801 tcaccatatt tttctggtaa gtgtcctgct caagggcgct catgcactga ttgaatgttt
     1861 gggcgggaca atacttgctc tggtcagcac aaccagcatt gtcaatttcg ttaatgcaat
     1921 cactctgaat gaattgatcg aggacccgaa cgatttcatc gccacacatt tgctcgcctt
     1981 ggcgcgagat ttttccgtcc agacgcagca cttctacgcc ttctatctac taagccatgg
     2041 tctcgtgaag gtgctgctcg tggtcggtct gctcagggag aaactttggt catatcccgc
     2101 atccctgatc gtattggcac tttttgtcgt ttatcagctc taccgtttct cttatacgca
     2161 cggatttggt ctaatcgtgc tgaccgtgtt tgacgtaatt gtgatgggcc taatttggca
     2221 cgaatataat ctcatgcgcc accatctgcc gacaaaatag agcctaccgg cggcaaaagg
     2281 ggattgccaa atatctgaga cggttgctcc gctcaggtcg taggccgatg gcatcacggg
     2341 taacaaggta aaaaggtcag ccacgccgag ccgcgcgccg ttccgggcca tccggcgaca
     2401 atccgattgc cgaacgagtt ttgtgaaggt gcaaaggtgg gaccgtgagc gggtgccgga
     2461 cggcgaattg gcgtagcctc tccacccgat tgagccgcgc cgccgcttca ccagcaaaga
     2521 cgagcacagt aacaatgaaa aactcgagga ccaagaggac ccacaaggta aatggcccgg
     2581 tcatgttcac ttgctccata attttttaaa tggctcaggt gacattgcgg tgtagcgggc
     2641 ggtgttcgta atcgaggcgt ggcccaagta gtgctgtaga cgcctcgctt ttgcagcacg
     2701 ctgcagcgac gatgcggtcg atcatatgcc gccgacttat ggctttcggg ttcaccacat
     2761 gcgatgcttt gcttcgaccg cgtttagacc gcgttaggca ttcggctgca cgtttgggct
     2821 gccactctgt tcaccggtag caaggtaacc tggttgggcg gactgaccgt cgctacgcgg
     2881 taaaacgctc tcacgcatcg tggcgcacga cac
//
LOCUS       DASRKK010000628         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_937807, whole genome shotgun sequence.
ACCESSION   DASRKK010000628 DASRKK010000000
VERSION     DASRKK010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937807"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFC15_15265"
     CDS             <1..279
                     /locus_tag="VFC15_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11565.1"
                     /translation="KNDRYVLVNNGMVVERGHLTLDPTQSPKTLDTTAMVGGDGDLPQ
                     VPARWIYSFEGDKLRLRGNGLPGKPRPQNFSSRPGDSFFTIEYRRAKP"
     gene            complement(1328..1759)
                     /locus_tag="VFC15_15270"
     CDS             complement(1328..1759)
                     /locus_tag="VFC15_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11566.1"
                     /translation="MRAEILNRCEAVEECYEFMLAYAAQGLPTDEGSKSGGQIREFLQ
                     RAVKALTGLAETCAMAVREEGLEPADKYVAFFAVLDRDSRDSLAAIELVLAQPAIGSQ
                     LIDNLNASIHLRALLTGLFLVDEILRKHDKPAEQVVTNKGD"
     gene            complement(1749..2621)
                     /locus_tag="VFC15_15275"
     CDS             complement(1749..2621)
                     /locus_tag="VFC15_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZM11567.1"
                     /translation="MDFVKWATSPWGQSVPIHIAWFLIWVAVIAGLLFLLVHAIYVRY
                     FAKQKEFAGNVSPAAAARIPEQVPRHSLTARIFHWIMAASMFTLLFTAFLPRVGVQFP
                     WVTYHWIAGAVLTVSIIFHIIHASFFMDFWAIWPDRIDLRDAWRRTLRSVGKAEPPPD
                     KFAKYPLENKLYHGAIIAAGLSTIVTGVFMMFRVRTVFFPRNPYLFSDMTWGMMYVLH
                     GLAGVGLIALIMVHIYFGLRPEKRAITKSMIFGWMSRDFYLKEHDPRRWVVETSSSPS
                     TSNRAEGRGRTDAG"
     gene            complement(2609..3625)
                     /locus_tag="VFC15_15280"
     CDS             complement(2609..3625)
                     /locus_tag="VFC15_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZM11568.1"
                     /translation="MAVIRDVMATFELYQPGSVADAQKLLARHGPDTLVLAGGLDSFD
                     WLKDRIKKPKTVVDLSGIEELRGVRTTGDGIEIGAMTTLTEVVNHPIVKQKYGLLAQA
                     AELVASPQIRNQGTLGGNVTQDTRCWYYRGGWPCYRAGGNVCYADTPEGRNREHAILH
                     ADRCVAVNPSDSAPALIALDAKFVVRTRKGERIVDAEDYFVGPEIDITRLHILQPGDL
                     LTAIRIPSKWAGAQFYFEKVRDRNVWDFPLMNVASAMVLSGNTIDRIRIAVNGAAARP
                     LRLKAVEDAVRGKPRDPATGEMAGRVAVQGAVPLQFNAYKIPLMRNLVKRAISGVEEA
                     TWTS"
     gene            complement(3646..>5366)
                     /locus_tag="VFC15_15285"
     CDS             complement(3646..>5366)
                     /locus_tag="VFC15_15285"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM11569.1"
                     /translation="QTLPAIARWLNIDPEKVVFISEYTGGGFGSKITGGVTMIIPALL
                     AKKTKAPVMMRISREEETFIGRARPSFQGRMKVGFSKEGRITALDMFVVSNNGPYDAQ
                     GDVPSSGRIVSLLYQPQAMRWRGVTVLTNTPPRSAQSSPGGLQGIVIMEPIIAKAGRK
                     LGVDQVAIRRINCPEGKAPFGPAVQGKRQYATSAFLKEALDRGAEQFKWSERIARTPK
                     RIGTKVRGVGVSLSCYVGGTIGFDGLLVIKPDGRINFQSGIGNLGTESVIDVHRVGAE
                     ILGVPWEKCDVTWGNTAKNLPFTCVSGGSQTTHAMTRAAYATAMDAKKKLQEIAAKKL
                     GGKPEQYEVANERVFRKGGGAGMTLAQAARHAIELGGVYDGHEAPEDIHKLTKASVAA
                     LAGQGLVAAAKDNYPRDGSTFSYVASFAEVEVDVETGKYYIVDFLASADVGTVIHPRA
                     LGGQVLGRSTLGIGHAIGQKWVFDPHYGVMLSTRFYQSKPPTILDVPVNMQWTALDIP
                     DPETPAGARGVGEPPVGGGCASVLNALSDALGDEIFQRAPVNADTILTSLEAGRPMQH
                     ALMAHI"
BASE COUNT         1094 a         1672 c         1460 g         1140 t
ORIGIN      
        1 aagaatgatc gctatgtgct ggtgaacaat ggcatggtgg tcgaacgcgg acatttgacc
       61 cttgatccca cccaatcgcc caagacgctc gacacgactg cgatggtcgg cggcgatggc
      121 gatctgcccc aggtccccgc acgatggatc tatagcttcg aaggtgacaa gcttcgtttg
      181 cgcggcaacg gactgcccgg caaacctcgg ccgcagaact tttcgtcccg tccgggcgat
      241 tcgttcttta cgatcgaata tcgccgcgcg aaaccctgag cgtcgactca cacacctggg
      301 attgcaagcc gagacagagg acccgtttct taccaaaaca tgacttcttg cagatgctgc
      361 tggcatcgta cctagctcca aagttatcca gccctgattc ggataccaaa attgacggag
      421 accacggtcg tgaacgtgat taagtaagca catcccgcga ctcttgcggc tttgcgttgt
      481 gactcgtcga tcatgctgat tgtcatttca tttcctccgg catcccgtat ctgctgtaga
      541 cggcggcttt cacgcgcatc ccgccgctgc gctggcctgc tccttccatc gttgttcgtt
      601 cacgccgacc acgaggagcc acaggcacag cgatagctcc gccgcgccgc aggaatccag
      661 ttcactctat gctaggacat ctaatggttg gccctttcca cccttaatcg aaagccacaa
      721 cataatcggc aattccccaa actgaagcac catcgcaaac tcggaaacga tatctccgta
      781 acgtgggaag agtaagtagg taacagtgac agccagatag gcgaaacctg cgggtatcag
      841 ccttcgcaga ccggtcttgg tcagttgacc aagacgtgca ggagatctcc cggttttcgt
      901 gcatgttgtt tctcagcgtg cgcgggttct tagactacgc aggaccgacc atcgactcgc
      961 gatagcgtgg ttgtcgtgtt gccttcctcc ataccggaac ggagtcggca tcctgatcca
     1021 tcggcttttc gaagctcaat agcccggcct accgatacct ctgtttacgc ttcaagcgac
     1081 acctcgcgat atcgcctgcg agactcgagg ccaggatgga ttcgctgctc tcctttcctg
     1141 tagggccctt tcatccccta caacatgccg gtttatcccg gcgttctccg gtttgccaac
     1201 aacgcaccca gaatcttaaa aaatcgggtc cttccgtgcg gacagaaaat ctgggcgact
     1261 cctgacacgg gcaacgctga tgactttctg actattcttc tgccaaagag gagctgggcc
     1321 gattgcctca gtcgcctttg ttcgtgacaa cctgctccgc cggtttgtca tgcttcctca
     1381 ggatttcatc caccagaaac aagccggtga gcagggcgcg gagatgaatg gaagcgttca
     1441 gattatcaat cagctgggaa ccgatggcgg gttgggccag aaccaactca atggcggcaa
     1501 gagagtcgcg agagtcgcga tcgagcaccg caaaaaacgc tacgtatttg tcggctggct
     1561 caagaccttc ctctctgact gccatcgcac aagtttccgc cagccccgtc aaggctttca
     1621 ccgcgcgctg caggaactca cggatttgtc cacccgattt gctgccttcg tcagtgggga
     1681 gaccctgggc tgcataggcc agcataaact cgtagcactc ttccacagct tcacagcgat
     1741 tcaatatctc agcccgcatc ggttcgccct cgaccctccg cccggttgga tgtcgatggc
     1801 gaagaagatg tctcgaccac ccatcgcctg ggatcgtgtt ccttcaagta aaagtcgcgg
     1861 ctcatccagc cgaagatcat cgatttcgtg atcgcgcgct tctccggccg gagtccgaag
     1921 tagatgtgca ccatgatcaa cgcgatcagt ccgacgcccg ccagtccgtg cagcacgtac
     1981 atcatgcccc aggtcatgtc gctgaacaga tacggattgc ggggaaagaa gacggtgcgc
     2041 acccgaaaca tcatgaagac acccgttacg attgtagaca gaccggcggc gatgatggcg
     2101 ccgtgataga gcttgttctc caaaggatat ttggcgaatt tgtccggtgg tggctcggcc
     2161 ttccccacag accgcagcgt tcttcgccat gcgtcccgca ggtcaatccg gtcaggccag
     2221 atcgcccaga aatccataaa gaaggatgcg tggatgatgt gaaaaatgat cgaaaccgtt
     2281 aacacagcac cggcgatcca gtgataggtc acccacggaa actggacgcc caccctcgga
     2341 aggaaggcgg taaacagcaa ggtgaacatg gaggctgcca tgatccaatg aaatatccgc
     2401 gcggttaacg agtgtctggg cacctgctca ggtatgcgcg ccgctgcggc cggcgaaaca
     2461 ttgcccgcaa actctttttg tttcgcgaaa tatcttacgt agatggcgtg gacgagcagg
     2521 aacagcaacc ccgcgatgac ggctacccag atcaaaaacc aggcaatatg aatcgggaca
     2581 ctctgccccc acggactcgt cgcccacttt acgaagtcca tgttgcttcc tccactccac
     2641 tgatggcgcg cttcaccaga tttctcatca gcgggatctt ataggcattg aactgcagcg
     2701 gaactgctcc ttgcacggct actcttcccg ccatctcacc agttgccggg tcgcgtggct
     2761 tgccgcgaac tgcatcttcg accgccttta gccttaacgg acgtgcggcg gcgccgttca
     2821 ccgcgattcg gatgcggtcg attgtgttgc cggataacac catggcagag gctacgttca
     2881 tgagcgggaa gtcccaaacg ttgcggtctc gcactttctc aaaatagaat tgcgcgcccg
     2941 cccacttgga cggaatacga atggcggtaa gcaggtctcc gggctgcaag atgtgcagcc
     3001 gggtaatgtc gatttcagga cccacaaagt agtcctctgc atccactatg cgctcacctt
     3061 ttcgcgtgcg cacgacgaac ttggcgtcga gggcaatcag ggctggggct gaatcggagg
     3121 gattcaccgc cacgcagcgg tcagcgtgca ggatcgcatg ctcgcggttg cgtccctccg
     3181 gtgtatcggc atagcaaacg ttaccgccgg cgcggtagca cggccaaccg cctcggtaat
     3241 accagcagcg cgtgtcctgc gtaacattgc cgccgagtgt tccttggttg cggatttgcg
     3301 gcgacgccac cagttccgcg gcctgcgcca gcagaccgta cttctgcttg acgatgggat
     3361 gattcaccac ctcggtgagc gtggtcatgg cgccgatctc gataccatcg ccggtcgtgc
     3421 gaactccgcg cagttcctcg attccgctca gatcgaccac agtcttcggc ttcttgattc
     3481 gatctttcaa ccagtcgaag ctgtcgaggc ctccggctag caccaaggtg tccggtccgt
     3541 gccgtgcgag aagcttctgc gcgtcagcga cggaacccgg ctgatacagc tcgaacgtgg
     3601 ccattacgtc tcgaatgaca gccatgactc tccttcaaac ctccatcaaa tatgcgccat
     3661 cagcgcatgc tgcatcggtc ggcccgcttc gagcgacgtc agaatcgtgt cggcgttcac
     3721 aggagcgcgc tggaaaatct cgtcccccag cgcatccgag agggcgttca agacggaggc
     3781 acacccacct ccaaccggcg gctcgccgac accgcgcgcg cccgccggcg tttccgggtc
     3841 cggaatatcc aacgccgtcc attgcatatt gacgggaaca tccagaatcg tcggcggctt
     3901 gctttgatag aacctcgtgg aaagcatcac gccgtagtgc ggatcgaaca cccatttctg
     3961 accgatggca tggccgatgc ccaaggtcga acggccgagt acctggccgc caagcgcacg
     4021 ggggtggatc accgtcccca catcggcgga ggcgaggaaa tccacgatgt agtatttgcc
     4081 ggtttcgaca tcgacttcaa cctcagcgaa gctggcgacg taggaaaacg tcgagccatc
     4141 gcgtgggtag ttgtccttgg ctgccgccac caagccctgg cccgccaacg ccgccacgga
     4201 cgccttcgtc aatttgtgga tatcttcggg cgcctcgtgg ccatcgtaaa cgcctcccag
     4261 ttcaatcgca tgtcgcgcgg cctgcgccaa agtcatcccg gctccgccgc ctttgcggaa
     4321 aacacgttcg tttgcgacct cgtactgttc cggtttgccg ccgagtttct tggccgcgat
     4381 ttcctggagc ttcttcttgg cgtccatggc cgtcgcatac gctgcccgcg tcatcgcgtg
     4441 ggttgtctgg ctgccgccgg agacgcaggt aaaaggaaga ttcttcgccg tattgcccca
     4501 cgtcacgtcg catttctccc atggcacgcc gaggatctcg gcgccaacgc gatgtacgtc
     4561 aatcaccgac tccgttccca ggttaccgat gccagactgg aagttgatcc ggccatccgg
     4621 cttgataaca agaagaccat cgaacccgat cgtgcctcca acgtaacagc tcagcgatac
     4681 cccgacgccg cgcactttcg tgccgatcct cttcggggtg cgtgcaattc tctcgctcca
     4741 cttgaactgc tccgcgccgc ggtcgagcgc ttctttgaga aacgcactgg tggcgtactg
     4801 ccgcttcccc tgcactgctg gtccgaacgg ggctttgccc tcggggcagt tgatgcggcg
     4861 gatcgccacc tggtcaactc cgagcttgcg cccggctttc gcgatgatcg gctccatgat
     4921 gacaatgccc tgcaacccgc ccggcgaact ctgcgcactt cggggcggtg tattcgttag
     4981 caccgtcacc ccacgccagc gcatggcttg tggctgatac aacagagaga cgatgcgccc
     5041 cgacgaaggg acatcgccct gtgcgtcgta tggaccgttg ttggaaacga cgaacatgtc
     5101 gagggccgtg attcgtccct ctttcgagaa gccgactttc atccttcctt gaaaactggg
     5161 acgcgcgcgt ccgatgaagg tctcctcctc gcggctaatg cgcatcatca ccggggcctt
     5221 cgttttcttc gccagcagcg cggggatgat catcgtcacg ccgcccgtga tcttgctgcc
     5281 gaacccgcct ccggtgtatt cgctgatgaa aacgactttc tctggatcga tgttcagcca
     5341 gcgcgcaatg gcgggcagcg tttgcg
//
LOCUS       DASRKK010000629         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1328834, whole genome shotgun sequence.
ACCESSION   DASRKK010000629 DASRKK010000000
VERSION     DASRKK010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328834"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFC15_15290"
     CDS             complement(<1..831)
                     /locus_tag="VFC15_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZM11570.1"
                     /translation="MTKRIAVVPGDGIGKEVIPNALEVVGASGAAVEFTTFDWGADRY
                     LLDGTTVPPDGFQMLARDFDAIFVGALGDPRVPSNIHAKEILLGMRFKMDLYANVRPV
                     RLLDASLCPLKDASSDDINFTVIRENTEGVYMDMGGAFKFGTPDEVATQEDINTRKGV
                     ERIIRFAFDYARANGRKRVMMSDKSNVMTYAGSLWQRVFKEVAKEYSDIQTQHMYVDA
                     LCLQMVREPSSLDVIVTNNMFGDIVTDLAAALQGGLGMAASGNIHPGRTSMFEPVHGS
                     A"
     gene            complement(1126..1398)
                     /locus_tag="VFC15_15295"
     CDS             complement(1126..1398)
                     /locus_tag="VFC15_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11571.1"
                     /translation="MMDKKKESDASEQNADIKKAYRADSKQRAPHLIDLDEQKQIEKL
                     RRSVVAAVKAGEGQKALDLLKQMGYGHDTAEYKAVIALLYPPSRRK"
     gene            1672..2739
                     /locus_tag="VFC15_15300"
     CDS             1672..2739
                     /locus_tag="VFC15_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11572.1"
                     /translation="MTVTTFQEKLAANGARLGMYRGAETPADFGDSTVEFRALLEGCA
                     LLDMSWQAKLVLAGEDRVRWLNGMVTNNVRDLAPGCGVYSFVLNAQGRIQGDLVAYNR
                     GDYLLATTDREQAPAIAEIFDRYIIMDDVEVADISDKLAAIGVDGPKAREILTAAGLD
                     VSQLEPGQVIDAVWRGVGISLARSAQPQMDGFEIWFASENVDAIWDALVSAGATPAGS
                     DALELYRIVRGIPRFGVDLRERDLPQETGQQHALNFAKGCYIGQEIVERIRSRGNVHR
                     MFTGFEVQGHPVAEPPAPGSKVRANDKDVGEITSAARIPFPSGERTLALGYARREAAL
                     PGATVQIGEQSATIQTLPFSE"
BASE COUNT          620 a          831 c          880 g          596 t
ORIGIN      
        1 cgccgagccg tgcaccggct cgaacatcga ggtgcggccg ggatggatgt tgccgctggc
       61 ggccataccc agcccgccct gcagcgcggc agccaggtcg gtgacgatgt cgccgaacat
      121 gttgttggtg acgatcacgt caagcgagct cggctcgcgc accatttgca ggcaaagcgc
      181 gtccacgtac atgtgctgcg tctggatgtc ggaatactct tttgccactt ccttgaagac
      241 gcgctgccac aggctgccag cgtaagtcat cacgtttgac ttgtcggaca tcatcactct
      301 cttgcggccg ttggcccgcg cgtagtcgaa ggcgaagcga atgatgcgct ccacaccctt
      361 gcgcgtgttg atgtcctctt gcgtggcgac ctcgtcgggc gtaccgaact tgaaggcgcc
      421 acccatgtcc atgtagacgc cttcggtatt ttcgcggata accgtgaagt tgatgtcgtc
      481 agacgaggcg tctttcaggg gacacagcga ggcatcgagc aggcgcaccg gacgcacatt
      541 ggcgtacagg tccatcttga aacgcatgcc gagcagaatc tccttggcgt ggatattgct
      601 gggcacgcgc ggatcaccca gcgcaccgac gaagatcgcg tcgaagtcac gcgccagcat
      661 ttgaaagccg tctggcggaa cggtggtgcc gtcgagcagg tagcggtcgg cgccccagtc
      721 gaaggtggtg aactcgacgg ccgctccgct ggcgccaacc acttcgagag cgttgggaat
      781 gacttctttg ccgatgccgt ctccgggaac caccgcgatg cgtttggtca tagtcagtga
      841 aaaacgataa cagaaatacc gtcggcggag ccgcttcaat ctttgccagg cgaccacagc
      901 cactggctgc gcatcagatg gttagagatg aggcccgtgg taagatgaaa ctgttaccaa
      961 ggaaaaatga gaacatggca accgctcctg cccgcaaaaa attgccggaa acgaactacg
     1021 catcgcgcac taacgctgaa gaaatagctg acttggtggt gggcgcgatc gaacagaggt
     1081 tatctgccct tccgaagcaa cctagaatga aagagcgtga ccgtctcatt tcctccttga
     1141 aggtgggtaa agcaaagcga taacggcttt gtactcagcg gtgtcatggc catatcccat
     1201 ttgttttaag aggtcgagcg ctttttgccc ttcgcctgcc ttgaccgcgg ccactacgct
     1261 gcggcgcaac ttttcaatct gcttttgttc atccaggtcg atgaggtgtg gggcccgctg
     1321 tttgctgtcc gctctgtagg ctttcttgat atctgcgttt tgctccgaag catccgattc
     1381 ttttttcttg tccatcattt cgaaccgcgc gcctagaacg attcaggttg cgacctcggt
     1441 gtcacgcggg tttcataata acaggctgat ccgaattgtc ttcgctagga tcaaagtatc
     1501 ggttgaaaga tagcagaaca actcaggatc tcaacgaacc caccatcaac gctaagccgg
     1561 ctgaactgaa tgctgagtgc tgttggctga gtactgatgg ctgacagctg agtgctgatt
     1621 tctgactgct gaatgctcct tcccttcctt cgctataatc aaagattcgg tatgactgtt
     1681 accacttttc aagagaagct tgcggccaac ggcgcacgac ttggcatgta tcggggcgcg
     1741 gaaacccctg cggacttcgg cgattccacg gttgaatttc gggcgttgct ggagggttgc
     1801 gccctcctcg acatgagctg gcaggccaag ctggtgctgg ccggagagga ccgcgtgcgc
     1861 tggctgaatg gcatggtcac gaacaatgtc cgcgatcttg cgccggggtg cggtgtgtac
     1921 agcttcgttc ttaacgccca ggggcgcatc cagggagact tggtcgcgta caaccgcggc
     1981 gactacctgc tagccaccac cgaccgcgag caagcgcctg cgatagctga gatcttcgat
     2041 cgttacatca tcatggacga cgtcgaggtc gccgacatca gtgacaagct cgccgcgatt
     2101 ggcgtggacg gcccgaaggc acgcgaaatt ttgacggctg ccggcctgga tgtctcgcaa
     2161 ctcgaacccg ggcaagtgat cgacgccgta tggcgcggag tcgggatttc cctcgcgcgt
     2221 agcgcccagc cacaaatgga tggattcgag atctggtttg cctcggagaa cgtggatgca
     2281 atctgggacg ccctggtctc ggccggcgcc acgcccgccg gcagcgatgc cctcgaactc
     2341 tatcgcatcg tcagaggaat accgcgcttc ggagttgatc ttcgcgaacg cgatttgccg
     2401 caggagaccg gacagcagca cgcgttgaat ttcgccaagg gctgctacat cgggcaggag
     2461 atcgtggagc gaatccgctc gcgcggcaac gtgcatcgga tgttcaccgg cttcgaagtg
     2521 cagggacatc cggttgcgga gcctccggcg ccgggaagca aagtccgtgc caacgacaag
     2581 gatgtgggag agataaccag cgcggcgcgg attccgtttc ctagtggcga gcggacgctg
     2641 gcgctcggct acgcgcggcg cgaagccgca cttcccggcg ccacagtgca gatcggcgag
     2701 cagagcgcca caatacagac ccttccattc agcgagtgaa taaatattta tggccgacaa
     2761 agagaagaaa cctgaattcg tagtaaccga tcgccgcttg ttcagcaccg atggcgagtt
     2821 acgccaggac gtggtggacg atgaagaacg ccgcgccgaa cgggaacgcg agaaaagtga
     2881 agcgcagcgc ctggtcaacg aggaacgcgc ggcccaacaa caacctg
//
LOCUS       DASRKK010000630         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_937897, whole genome shotgun sequence.
ACCESSION   DASRKK010000630 DASRKK010000000
VERSION     DASRKK010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937897"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..895)
                     /gene="phoU"
                     /locus_tag="VFC15_15305"
     CDS             complement(227..895)
                     /gene="phoU"
                     /locus_tag="VFC15_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZM11573.1"
                     /translation="MRSRFQQGLDELKEKLLRMGGLAEQAIDRATEAYRTRDSKYCQM
                     VLTGENAINEAEREIDELSLDLLAMQQPMAVDLRFILAVLKINADLERVGDQAVNIAQ
                     RVLDLISEPEVQLPVDIPRMADSVSTMVQRALEAFLDGRAEVAEAVLQMDGIVDRMKD
                     EAFIVLVQKMYNEPTVTRAALNVLLISRNLERIADHATNIAEDVIFWVRGADVRHGGA
                     HYRQ"
     gene            complement(895..1656)
                     /gene="pstB"
                     /locus_tag="VFC15_15310"
     CDS             complement(895..1656)
                     /gene="pstB"
                     /locus_tag="VFC15_15310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZM11574.1"
                     /translation="MGVGIEVRGLNAWYGKNQSLNSIDMTVAANHVTAVIGPSGCGKS
                     TYVRCLNRMHETIPEARAEGSVKVGEMDVYGSGSDPVEVRRRIGMVFQKPNPFPTMSI
                     YDNVAAGLKLNGFRNRKQMNELVERSLKLAALWEEVKDYLHKKSGASLSGGQQQRLCI
                     ARALAVEPEVLLMDEPASALDPISTSKIEELIFQLKQHYTVVIVTHNMQQAARVAEFT
                     AFFLMGSLIEYDKTEKIFTTPADKRTEDYITGRFG"
     gene            complement(1665..2540)
                     /gene="pstA"
                     /locus_tag="VFC15_15315"
     CDS             complement(1665..2540)
                     /gene="pstA"
                     /locus_tag="VFC15_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZM11575.1"
                     /translation="MPNPAHNSATHELGWLRRAADHLATGFAGLAAILVIVPLLAIFA
                     YLVIKGVGSINIAFLTQTPKPPGEAGGGMANAIVGTGLILAVSSLIGVPMGIGSGIYL
                     AEYGRNRFGDLVRFTADVLNGVPSIVIGIAVYSLVVVPQKHFSAFSGGVALGIMMIPT
                     VARATEEMLLMVPNVVREAAYGLGIPKWRTTLSISLRTASAGVITGCMLAFARVAGET
                     APLLFTAFGNQFWNLKLNQPTAALSLQVFTYAISPYDDWHKQAWAGALILIVLIVAAV
                     AAVRITTSRGMMKGA"
     gene            complement(2533..3588)
                     /gene="pstC"
                     /locus_tag="VFC15_15320"
     CDS             complement(2533..3588)
                     /gene="pstC"
                     /locus_tag="VFC15_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZM11576.1"
                     /translation="MNPTEPLGQIQKPATAIPSMAQVSRERKPAASTVLQKIRTSQPR
                     ASRIPDAVFRVIIVLSAVSVFAIVVLVAWELIDKSRLSLHQFGIGFFYGHDWDPVNGS
                     FGAMPFIYGTLVSSFLALLLAVPLSLGVAVYITEMCPQRLRAIISFLVELLAAIPSVI
                     YGLWGIFVLAPLLREYVEPFLARTLGWTGFFSGPMYGLGMLAAGIIMAIMVVPIIASI
                     TREVLTAVPQNQREAVLALGATRWEMIRMGVLRNARIGIVGGIILGLGRALGETMAVT
                     MVIGNRPEVAKSLFAPGYTMASVIANEFSEATGDLYLSALVEIGLALFLVTLIVNALA
                     RLLVWSITRGSPGRTHA"
     gene            complement(3813..>4133)
                     /locus_tag="VFC15_15325"
     CDS             complement(3813..>4133)
                     /locus_tag="VFC15_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11577.1"
                     /translation="VQNSSKNFIKASLDSTTAAAVGVKMPADFRVSITNPPGKNAYPI
                     ASFTWLLVPTNPADANKGKILKDFLFWMLDKGQTMTEALSYAPLPTQVVEMEKAAITK
                     QIKP"
BASE COUNT          875 a         1248 c         1177 g          833 t
ORIGIN      
        1 cgcttcagcg gtgcagttta agattcagaa atgagccggt tttagccact gaggtgacgg
       61 aaacattacc tcaggcgcta aagcgcgatc caggaactga cggcagttat gcacgcctga
      121 aaggcgtgct ccacccatcc ctatacacca gatgtgaaac tgctatagtc gtagccgcag
      181 tttccggcgg cactgatcgg acccatgcgc ttcccaccgc gttcaattat tgccggtagt
      241 gagcgcctcc gtggcgcacg tcggccccac gcacccagaa aatcacatct tcggcaatat
      301 tggtggcgtg gtcggcgatg cgctccaggt tgcgggagat gagcagtaca ttgagcgccg
      361 cgcgcgtcac cgtaggttcg ttgtacatct tctggaccag cacaatgaag gcttcatcct
      421 tcatgcggtc cacaatgccg tccatctgga gaacggcctc ggccacctcg gctctgccgt
      481 ccaggaaggc ctcgagcgcg cgttgcacca tggtgctaac gctgtcggcc atgcgcggaa
      541 tatccacggg caactgcact tcgggttcgc tgatcaggtc gagcacccgt tgcgcaatgt
      601 tcaccgcctg gtcgcccacc cgctccaagt cagcgttgat cttcagcacg gccaggataa
      661 agcgcagatc gaccgccata ggctgctgca tggccagcag gtcgagcgag agttcatcga
      721 tctcgcgttc ggcttcgttg atcgcgttct ccccggtgag caccatctgg cagtacttgc
      781 tgtcgcgggt acgataggct tcggtcgcgc gatcgatggc ctgctcggca aggcctccca
      841 tccgcaacag tttttctttc agttcgtcca gtccttgctg aaagcggctg cgcattatcc
      901 aaacctgcct gtaatgtagt cttccgtccg cttgtcggcg ggggtggtaa aaatcttttc
      961 cgttttgtcg tactcgatga gactccccat caggaaaaac gccgtgaatt cggccacgcg
     1021 agcggcttgc tgcatattat gggtcacgat gaccacggtg taatgctgct tcaactgaaa
     1081 tatgagttcc tcgatcttac tggtcgagat gggatccaag gccgatgccg gctcatccat
     1141 caacagtact tcgggctcga ccgcaagggc gcgcgcgatg cacaaccgtt gctgttgccc
     1201 gccggacagg cttgcgcccg acttcttgtg caaatagtcc ttcacttctt cccacaaagc
     1261 ggccagcttc agcgaacgct ccaccagttc gttcatctgt tttcggttgc ggaagccgtt
     1321 gagcttcaag cctgccgcga cattgtcata gatcgacatg gtgggaaacg gattcggctt
     1381 ctggaaaacc atgccaatgc ggcggcgtac ctcgaccggg tccgagccgc taccgtaaac
     1441 atccatctcg ccgactttaa ccgagccctc ggcgcgtgct tcgggaatgg tctcgtgcat
     1501 gcgattgaga cagcgcacgt aggtgctctt gccacatccc gaggggccga tgaccgcagt
     1561 tacgtggttg gcggccacag tcatgtcgat ggaattcagc gactggttct tgccatacca
     1621 ggcgttcaat ccacgcactt cgattccgac acccatacga gcttttacgc tcccttcatc
     1681 attccgcggc tggtggtgat gcggactgcc gcaaccgcag ccacgatcaa gacgatcagg
     1741 atcaaggctc cggcccacgc ttgcttgtgc caatcgtcat agggcgaaat ggcgtaggta
     1801 aaaacctgta acgacagcgc tgccgtcggt tgatttaact ttaagttcca aaactggttt
     1861 ccgaaagcgg taaagagcaa tggggccgtt tctcccgcca cccgggcgaa ggccagcatg
     1921 cagccggtga tcaccccggc ggaagcggtg cgcagcgaga tcgagagggt cgtgcgccac
     1981 ttgggaatgc ccagtccgta ggcggcttcg cgtaccacat tcggcaccat cagcagcatc
     2041 tcctccgtcg ctcgcgcgac cgtggggatc atcatgattc ccagcgctac gccgccggaa
     2101 aatgccgaga agtgcttctg cggaaccacg accagggaat agacggcaat gccgatcacg
     2161 atcgagggca cgccgttcaa cacgtcggcg gtaaaccgca ccagatcgcc aaagcgattg
     2221 cgcccatatt cagccagata aatgcccgag ccgatgccca tcggcacacc tatcaggctg
     2281 gagaccgcga gaatcagtcc ggtgccaacg atggcattgg ccatgcctcc gcccgcctca
     2341 cccggaggtt tcggggtctg ggtgaggaag gcgatgttga tcgagcccac tcccttaatc
     2401 accaggtaag caaagatggc gagcaaagga acgattacga gaatcgcagc gaggccagcg
     2461 aagccagtgg ccagatgatc ggcggcacgc cgcaaccagc caagctcgtg cgtcgcgctg
     2521 ttatgggccg ggttaggcat gagtcctccc cggggacccg cgggtgatgc tccaaaccag
     2581 cagccgcgcc agagcattca cgatcagcgt aaccaggaac agggccaacc cgatttccac
     2641 caacgcactc aggtagagat ctcccgtggc ctcgctaaac tcgttggcga tcacgctcgc
     2701 catggtgtag cccggtgcga acagcgactt cgcgacctcg gggcggttgc ctatcaccat
     2761 ggtcacggcc atggtttcgc ccagcgcgcg tccgagtccg aggatgattc cgcctacgat
     2821 gccaatgcgc gcgttccgca gaacgcccat gcgaatcatt tcccagcgag ttgcacccag
     2881 cgccagcacc gcctcgcgct ggttctgagg gacagccgtc agcacctcgc gcgtaatgga
     2941 agcgatgatg ggcacaacca tgatggccat gatgatgccg gcggccagca tccccagccc
     3001 gtacatcggt ccgctgaaga agcccgtcca gccgagcgtc ctggcaagaa acggctcaac
     3061 gtactcacgc agcaggggcg ccagtacgaa gatgccccac aatccataaa tgacgctggg
     3121 aatggccgcc agcagttcca caaggaacga gatgatggct cgcaggcgtt gggggcacat
     3181 ctcggtgatg tagactgcca cgcccagcga gagtgggacc gcaagaagga gtgccaggaa
     3241 agacgaaacc agtgtgccgt aaatgaacgg catcgcccca aaactgccgt ttaccgggtc
     3301 ccaatcgtgc ccgtagaaga aaccgatccc gaattggtgc agcgacaagc gggacttgtc
     3361 gatcaactcc cacgccacta gcacaacaat cgcaaacacc gagaccgcgc tcaaaacgat
     3421 aatcacgcga aagacggcgt ccggaatgcg gctggcgcgg ggctggctgg tgcgaatctt
     3481 ctgcagcacg gtagaagctg ccggcttccg ctccctgctc acctgcgcca tcgatgggat
     3541 cgctgtcgcg ggtttctgga tctgtccaag cggttcggtc ggattcattg ggtcgacgct
     3601 atcagacatg gattacagga gcgttacaaa gcgagcaact agcaactagc gattagaccg
     3661 cagcattcgc gtttgctggc tgtttgtacc cagtcatcag tagctatctt taagaacttt
     3721 tgttttgcta gccgctgcct gctcgttact agggtccgcg ccctttcaaa ggcggattta
     3781 cgaggcagct tctaattgct agttgctagt tgctaaggct tgatctgttt ggtaatggcg
     3841 gccttctcca tctccaccac ctgcgtgggc aggggagcgt aagagagcgc ctcggtcatg
     3901 gtctggccct tgtccagcat ccagaagaga aaatccttca ggatctttcc cttgttggca
     3961 tccgccggat tggtcggaac caatagccag gtgaagctgg cgatagggta agcattcttg
     4021 ccaggaggat tggtgatcga cacccggaag tcagccggca tctttactcc caccgcagcc
     4081 gccgtcgtcg agtccaggct ggccttaatg aagttcttcg aggagttctg cac
//
LOCUS       DASRKK010000631         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_58949, whole genome shotgun sequence.
ACCESSION   DASRKK010000631 DASRKK010000000
VERSION     DASRKK010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58949"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..620
                     /locus_tag="VFC15_15330"
     CDS             177..620
                     /locus_tag="VFC15_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZM11578.1"
                     /translation="MEYPQQAIKPSTPNLHPTVMRANTVLLALVLSGGQIVLTGCIAC
                     NTKPPNPDQVREKTAQATAELKDNAKAVAQGIREGLNRPTSDHPLDLNSASKSQLMSL
                     PGIDDAAADRIVAARPYDNTHQLLDKRIISRDEYSKIADSITVQK"
     gene            complement(862..2190)
                     /gene="ltrA"
                     /locus_tag="VFC15_15335"
     CDS             complement(862..2190)
                     /gene="ltrA"
                     /locus_tag="VFC15_15335"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM11579.1"
                     /translation="MSLTTPESIQKLQTALHAKAKESPDFRFYALYDKVYRKDILAFA
                     YACCKANGGAAGVDHQEFEDIEAYGAERWLDELSQELKNRTYQPLPVRRVYIPKADGK
                     TQRPLGIPAIRDRAAQVAAVLVLEPIFEADLQPEQYAYRKDRSALDAVRHVHKLINTG
                     HREIVDADLSSYFDEIPHAELLKSVARSVVDGAILHLIKMWLEAPVEETDERGNQRQS
                     TRNRDKGRGTPQGSPISPMLSNLYMRRFVLGWKRLGHEKRLRAYTVNYADDLVICCRS
                     GADQALAVMRNMMSKLKLAVNESKTRVCRLPEEKFDFLGYTLGRCYSAKTGKAYLGTV
                     PSKKRVLRLCQAVSEVTGRHSTSQDAETIVGTLNRKLTGWSNYFCLGPVSNAYRAVDR
                     HTVRRLRQWLRTKHKVQGRGTQRFSEDVLYSKFGLVRLKLRTASFPCATP"
     gene            complement(3035..>3336)
                     /locus_tag="VFC15_15340"
     CDS             complement(3035..>3336)
                     /locus_tag="VFC15_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11580.1"
                     /translation="VAYGTNELLIDKYFLFQVGYIRQLAKLPPLLGSGIYFLGVYEAA
                     QVYGPPHTMVNQASGFPTDAAAGLVVNTIFGPVEAAYAYGDTGHRKFFFRVGRLF"
BASE COUNT          653 a         1058 c          907 g          718 t
ORIGIN      
        1 cgttcgcagg gttgagtttc tcaggagcag tacgccgtcg ggatggcgag cgccacatag
       61 agcgtaccgc cggcatcttg tagacgagcc agttgacaga agcgaacaaa agggcacagt
      121 ccgcgggctg tgcccttttg cgtggatgga accagcaggg tcgagcatga cctcccatgg
      181 agtatccgca gcaggcgatc aaaccttcga ctccgaatct gcatcccaca gtcatgcgtg
      241 cgaataccgt cttgctggcg ctggttctga gtggcggcca gattgtgctt accggttgca
      301 tcgcctgtaa taccaagcca cccaatccgg accaagtaag ggaaaagact gcacaagcga
      361 ccgcggaact aaaagacaac gcgaaggcgg tcgcacaagg catacgcgag ggcctgaacc
      421 ggcctacgtc cgatcatccc ctcgacctga actctgcttc gaagagccag ctcatgagcc
      481 ttcccgggat cgacgatgcc gcggccgacc ggattgtcgc ggcgcggcct tacgacaaca
      541 cgcaccaact gctcgataaa cgaatcatct cgcgcgacga gtacagcaaa atcgccgaca
      601 gcattacggt gcaaaagtag cttcaccccg tcgcttgccc acacgtttgg ggcagttcca
      661 gaattggtgt agtccggcgc gtacgggtgg agcgggtctc catcgtgaag tcgaggtgtg
      721 gcgcggtggt ttagattcag cctatctgtt tccggccctt tcgtctgccg gtgcctcaat
      781 agcctcacca tgccccgttt ccacaccccg cttgtcgaac cgtgcgtgcg gatttcccgc
      841 acacggctct cggaggaagc gtcacggtgt cgcacacgga aaactggcgg tccgcaactt
      901 gagccggacc aagccaaact tcgagtacag gacatcctca gagaaccgct gagttcctcg
      961 tccttgtact ttgtgtttgg tgcgcagcca ctgtcgtagc cgccggacgg tgtgtctgtc
     1021 caccgctcgg taggcattgc tgacaggacc gagacagaag tagttcgacc aacccgtgag
     1081 tttccggttc agcgtcccta ctatcgtttc cgcatcctgc gaagtactgt gtcggccggt
     1141 gacttcactc accgcctgac agagacgtag cactcgcttc ttcgacggga ctgtgcccag
     1201 gtaagccttg cccgtcttag ccgagtagca acgccccagc gtgtacccca gaaaatcaaa
     1261 tttctcttcc ggcaaacggc agacccgcgt cttcgattcg ttcaccgcca gcttcagctt
     1321 cgacatcatg ttccgcatca cagccagggc ttgatcagcc ccgctgcgac agcagatcac
     1381 caggtcatcg gcgtaattga cggtgtacgc ccggaggcgc ttctcgtgcc ccaaccgctt
     1441 ccatcccagc acgaaccggc gcatatagag attgctcagc atcggtgaaa tcggagaccc
     1501 ttgtggagtc ccgcgaccct tgtcccgatt gcgcgtgctc tgacgttggt tcccccgctc
     1561 gtcggtttct tcaaccggag cttccagcca catcttgatc agatgaagta tggctccgtc
     1621 gaccacgctc cgagccaccg actttaaaag ctcggcgtgg ggaatttcgt caaagtagct
     1681 gctgagatcc gcatcgacaa tttctctatg cccagtgttg atcagcttgt ggacgtgcct
     1741 gacggcatcc agcgcgctgc ggtccttgcg ataggcatac tgctccggct gcagatcggc
     1801 ctcgaagata ggttcgagta ccaaaacagc agccacttgc gccgctcggt cgcggatagc
     1861 gggtatgccc aacggccttt gcgttttccc gtccgccttg gggatgtaga cccgtcggac
     1921 aggtaacggt tgataggttc gattcttcag ctcttgcgac agttcgtcca accatcgctc
     1981 tgccccgtag gcctcgatgt cctcgaactc ctggtgatcg actccagctg cgccgccatt
     2041 agctttgcag caggcatatg cgaacgccag aatgtccttc cggtacacct tgtcatacag
     2101 agcgtagaaa cgaaagtcag gcgattcctt cgctttcgcg tgtaacgccg tctgcaactt
     2161 ctgaatactt tctggagttg ttaggctcat cgccaatctc tcttccttcg ttgcttctat
     2221 tgcgttaact tccaactgag gccccttccc tccaccggcg ttacccggct tcctcggtac
     2281 tacgcgcctc cccgccaccc caaacggccc ggcctggctc tcgcgagttg ccggttgatc
     2341 ctgaaccgcc aggatcaccg aatagggctt cccgtgttgc gtctggtccc ctctgcgtgc
     2401 atgccatcgc cagtaccccg gcaagaccga tagaactcca ttcgctcgta ccgttccatc
     2461 gaccgcggcc ttcccctgaa aacaggaggg tcggctcttg cattagtcgt ttcgaggcct
     2521 gctcagcgtt tactcaggtt atggcctgca cgctcgccaa gtcgccatcg cgaccctcta
     2581 caccgaaggc ttcagcggct tcgttgcctc cgccgctgcc tcgattgcta ccgggtggag
     2641 cgaaccagtt cccgggcggg tctgtctccc gctgtggagc agcgcctttt cacggcgcac
     2701 tgttctcggt agctgaagtc agctacttga agggggacgg ctttcagccg tgccgttacg
     2761 tgtgcgtagg gagtcgggct tcagccctga gggaactcgc gcttcgcctc aacaccgaag
     2821 tccctcagtg gctaaagcca ccgtaataac agcagcttac ggcacggctg aagccgtgcc
     2881 ccttcaaagc gtgaggtcct cttgaataat ccaggtgaaa atccgcacta cccgtccgtt
     2941 ttttccgcag ccggtcgagt cccgcaatgt ggggcgatct tggacgccag cctgaaggct
     3001 ggctctaccc ggtctgcgca cactttgaaa ccgctcagaa cagacgccca accctgaaaa
     3061 agaacttgcg atggccggtg tcgccgtagg cgtacgccgc ctccaccggg ccgaagatcg
     3121 tgttgaccac caaacccgcc gctgcatcgg tggggaaacc cgaggcttgg ttcaccatgg
     3181 tgtggggcgg gccgtaaact tgcgcggctt catagactcc gaggaaatag atgccgctgc
     3241 ccagcaacgg tggcagcttc gccagttgcc gtatgtatcc cacctggaac aggaagtact
     3301 tatctatcag caattcattg gtgccgtatg ccacca
//
LOCUS       DASRKK010000632         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_253323, whole genome shotgun sequence.
ACCESSION   DASRKK010000632 DASRKK010000000
VERSION     DASRKK010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253323"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFC15_15345"
     CDS             <1..430
                     /locus_tag="VFC15_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11581.1"
                     /translation="FGVWFWQKAPSFLRLGSPRQVFHAADWLLWFLLWVLAPAVWLPI
                     ATTVAAAGFRLRGMARSLRVLRRPAYWLWFCALMLVGAYVPYRLVWWTPDLSDLRKQA
                     WSMGLRFFLAYVILITAFVALLLVVGSRTEKEDPEPIELG"
     gene            536..685
                     /locus_tag="VFC15_15350"
     CDS             536..685
                     /locus_tag="VFC15_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11582.1"
                     /translation="MSLQQRGKWREEGRERAWKLPPQAPDEGESLSGGWDEAKQGQNA
                     LAISA"
     gene            complement(811..2223)
                     /gene="selA"
                     /locus_tag="VFC15_15355"
     CDS             complement(811..2223)
                     /gene="selA"
                     /locus_tag="VFC15_15355"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZM11583.1"
                     /translation="MTSDLFRLLPSIDELLRNPVLDTLAQREGRSATTEAARVALEQL
                     RADIGAGKLDQAQIRFRLDDLPREVEKKLLESLAYSLRPVINATGVILHTNLGRAPLS
                     RAALEHLAEVSEGYSNLEFNLSTGERGKRDVHVDRLFAKLLNTAGREVSTIVVNNNAA
                     AVLLALNSLAEGGEVLVSRGELVEIGGSFRIPDVMAKSGAVLREVGTTNRTRIGDYKA
                     AISESTKLLLRVHRSNFQIVGFTEQPSLEELVALGRKRNVPVMEDLGSGEIFDLRNVG
                     VRGEPMIADSLRAGVDVVTYSGDKLLGGPQAGIISGNPALVAKVRGNPLFRALRVDKM
                     FYAALEATLLAYLREDYDSIPALRMMRISEDALAQRAKQIAERLRASCPNLRVEVVES
                     RSVLGGGAAPGSTLPTRALAIKSASMNADELCARLRQWETPIIARVEEGRVLLDLRTV
                     EPIQEEAIVAALHLDYSRSR"
     gene            complement(2571..2774)
                     /locus_tag="VFC15_15360"
     CDS             complement(2571..2774)
                     /locus_tag="VFC15_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11584.1"
                     /translation="MSFEEALGLASRDESFKATVYAMNTLLIGKGIYTQEEFESLFVE
                     WVRKEQRRSRSRAQSQDSMPCPA"
     gene            complement(2884..>3390)
                     /locus_tag="VFC15_15365"
     CDS             complement(2884..>3390)
                     /locus_tag="VFC15_15365"
                     /inference="COORDINATES: protein motif:HMM:NF040055.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZM11585.1"
                     /translation="TMASFQSGEIDVLVSTTVIEVGVDVANANIMVVEHAERFGLSQL
                     HQLRGRIGRGSAKSYCVLMTGGKVSVEAEQRLEAMVRTQNGFEIAELDLQQRGPGEFF
                     GTKQAGMPNFRVANLLRDRELLEAARREAKYVLAGPSPDLTKEEIARAVQHLRGHWNR
                     RYGLVEVG"
BASE COUNT          691 a         1015 c          984 g          700 t
ORIGIN      
        1 gttcggagtg tggttctggc agaaggcgcc gtcattcttg cgcctcggca gccctcggca
       61 ggtattccac gccgccgact ggctgctgtg gttcctgctg tgggtgctgg cgcctgccgt
      121 gtggctgcct atcgccacca ccgttgcggc ggccggattc agattgaggg gcatggcccg
      181 ctccctgcgt gtcttgcggc ggccagcata ctggctatgg ttctgcgcgc tcatgctagt
      241 cggggcgtac gttccgtaca ggctggtgtg gtggaccccc gacctcagcg acctgcgcaa
      301 acaggcatgg agcatggggc tccggttctt cttggcttac gtgatcttga taacggcatt
      361 cgtcgcgctg ttgttggtcg tcggttcgcg cacggagaag gaagatcccg agccgattga
      421 gctgggctaa cagagcgccg agaaagtctg taaaacaagc ctgtcgtcgg ctgaaagccg
      481 actccacctg acaaaaaata aacggctcgg acgcgggccc tcaccaggat tattcatgag
      541 cctgcagcag cgaggcaaat ggcgggagga gggtagggaa agggcgtgga agttgccgcc
      601 gcaagctccc gatgagggcg aaagccttag cggcggctgg gacgaggcca agcagggtca
      661 aaacgcacta gcaatttctg cttgaggtag aaagaaaagt gcagttgtgg tccaatacag
      721 tctgtcattg gcggatgctc ccaaaagatg ttctggattc cacgaaaatc cagtgttcat
      781 acacctttta gagcatccgt tgttttttgc ctagcgagac cgtgaataat ccagatgcag
      841 ggccgccacg attgcctctt cctgtatggg ctcaacggtg cgcagatcga gcagtacgcg
      901 tccctcttcc acgcgcgcga tgatcggcgt ctcccactgg cggagccgtg cgcagagctc
      961 atccgcgttc atgctcgcgc tcttgatggc gagcgctcgc gtaggcagag tcgatccagg
     1021 agcagcgccg ccgcccagca ccgagcgaga ctcgacaact tcgacccgca agtttgggca
     1081 gctcgcacgc aatcgttccg ctatctgctt cgcgcgctgc gcgagtgcgt cttcggagat
     1141 gcgcatcatg cgtaacgccg gaatcgagtc atagtcctcg cgaagatacg cgagtagcgt
     1201 cgcttcgagc gcggcataga acatcttgtc aacgcgcagg gcgcggaaca ggggattgcc
     1261 gcggaccttc gccaccagcg cgggattgcc gctgatgatg ccggcttgcg ggccgcccag
     1321 cagcttgtct ccactgtagg tcacgacatc cacgccagca cgcaggctgt cggcgatcat
     1381 cggctcgcca cgtacgccga cgttgcgcag gtcgaagatc tctccgctgc ctaggtcttc
     1441 catcaccgga acgttgcgct tgcgtccgag cgcgaccaac tcttccagcg acggctgctc
     1501 ggtaaagccc acaatctgga agttggagcg gtgcacgcgc aacagcagct tggtactttc
     1561 ggagattgcg gccttgtagt cgccaatgcg cgtgcggttg gtggttccca cttcgcggag
     1621 caccgcgcct gacttcgcca tgacatctgg aatgcggaac gagccaccga tctcgaccag
     1681 ttcgccgcgc gagaccagca cctctccgcc ttcggccagc gagttcagcg ccagcagtac
     1741 cgcggcagcg ttgttgttga ctacgatggt cgagacttcg cggcctgcag tgttaaggag
     1801 tttcgcaaat aagcggtcga cgtggacgtc gcgcttgccg cgctcgccag tgctgaggtt
     1861 gaattcgagg tttgagtatc cctccgagac ttcggcgaga tgttccaatg ctgctcggct
     1921 gagcggcgca cgtccgagat tggtatgcaa gatgacgcca gtcgcattga tcacgggacg
     1981 taacgagtaa gccagcgatt cgagcagttt tttctccacc tcccgtggca agtcatcgag
     2041 cctgaatctt atttgtgcct ggtcgagctt gcccgccccg atgtctgcgc gcaattgttc
     2101 cagtgctacg cgggctgctt cggtagtcgc gctgcggcct tcgcgttgcg ccagcgtgtc
     2161 caacacagga ttgcgcagca actcgtcaat cgagggcagc aggcgaaaga ggtcagaggt
     2221 catatattct gtcattgaca gagggtcgag cagatctcag aaaaagagca cgatcagtat
     2281 gccactttcg cttttctttt ccgctgcgac acacagcaat ccccactttt ccacaggctt
     2341 tccacgcttc tgcgtaaagt tgccattgct atctactgag ataatttgag gtaatgctat
     2401 atgcatgaag attgtggcat atagccgcag tcgctagaga aaggcctcga tgacagccaa
     2461 ggtgaagagg gagttgaaac ctgcaccccg ctgcgcctgc gaatgcaagg cgtgtgacat
     2521 cggaatacat tgtggccgca agcctgactg cgaacatccg acctaccgct ctaagccggg
     2581 caaggcatag agtcctgaga ctgcgcccgc gatcgggaac gtcgctgctc tttgcgcacc
     2641 cactccacga acagcgactc aaactcttcc tgagtgtaga tacctttgcc gatcagcaga
     2701 gtgttcatcg cataaacggt cgctttgaac gattcatctc ttgacgcaag acccaacgct
     2761 tcctcaaacg acatcgcatc ccccagcacg acagctaaaa actgcaagca ttaaaccata
     2821 agaatatccc aaaagcagag aacaatattc acccagagcc cgggtaacaa gtaacttccg
     2881 gtttcacccc acctccacca gcccgtacct ccggttccag tgtccgcgca ggtgctgcac
     2941 ggcgcgggcg atctcctctt tggtcagatc ggggctgggg ccagcgagca catatttcgc
     3001 ctcgcgacgc gcggcctcca gcaattcgcg gtcgcgcagc aaattggcaa cgcgaaagtt
     3061 cggcatgccc gcctgcttgg taccgaagaa ctcgccgggg ccgcgctgct gcaagtcgag
     3121 ttcggcaatc tcgaatccat tctgagtgcg caccatggct tcgagccgct gttcggcttc
     3181 caccgaaact ttgccgcccg tcatcagcac gcaatacgac ttagcgctgc cgcggccgat
     3241 gcgcccacgc agttgatgca gttgcgagag accgaaacgt tcggcgtgct ccacgaccat
     3301 gatgttggcg ttggccacat ccacgccaac ctcgatgacc gtagtcgaga ccagcacatc
     3361 aatttcgccg ctctgaaagc tggccatggt
//
LOCUS       DASRKK010000633         7819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1428068, whole genome shotgun sequence.
ACCESSION   DASRKK010000633 DASRKK010000000
VERSION     DASRKK010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428068"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFC15_15370"
     CDS             complement(<1..215)
                     /locus_tag="VFC15_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HZM11586.1"
                     /translation="MGTRPAIFKWRQTESGLILCAVRWYLRYSLSLRDVEELLEERGL
                     NVDHTTVWRWVQYYGPELEQRLRKHLKP"
     gene            523..1317
                     /locus_tag="VFC15_15375"
     CDS             523..1317
                     /locus_tag="VFC15_15375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZM11587.1"
                     /translation="MRLLGVLLCYNDGDFLEDSIRYLLEQNHHLIVWDHGSTDETADI
                     IRQYKNDLLESRYVPREFDFYELYPAMSRHLTDNYSSIYDWISWPDQDEILEGPSRSK
                     PYREWLEDVHASSCNWIQFNNYNYWFTSSDDTAIKSPGARIRHYSIFPDCAPRIRAWR
                     ASATNIRIFNHNPPLGDPWPARFNLRHYSMRSPQQMLARVLKDRSNLRRGGDNYHYDN
                     MKLNLDRLTISPDQLHYDDGRSDLDPSPVFNWRSIYGSGPPPDPVP"
     gene            complement(1277..2065)
                     /locus_tag="VFC15_15380"
     CDS             complement(1277..2065)
                     /locus_tag="VFC15_15380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM11588.1"
                     /translation="MGVVLPSSEYDDELLKIHLQQASEALPGPDYYTVLRWIHEILRP
                     ANYVEIGVRQGGSLRAALPDTTCIAIDPLPALAGPLPPRTHVLPITSDAFFESYNPCD
                     LLGTPHFSLAFIDGLHLFEQVLWDFIHLERLAGSSSIIILHDCLPLDRITSERVRTTH
                     FYSGDVWKLPMCLKVHRPDLRMVTIRTPPTGLCLVSRLDPGSDLLVRNYAEYVAEYTP
                     LDFDYYKDHPEQMPENIPNTFAAVSGWIDSLIRERDPAVDRSHK"
     gene            2132..3202
                     /locus_tag="VFC15_15385"
     CDS             2132..3202
                     /locus_tag="VFC15_15385"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM11589.1"
                     /translation="MATSDKILAMIVWLRHFLGWLRGAFSAREDLILENLALRQQVLA
                     LHAQRPRRRLTASHKLFWVVLRKLWARWKRPLILVTPRTVVGWHRAGFRLYWKWLSRP
                     RSAGGRKRISEEIRTLIFRMVAENPTWGAPGIHGELLKLDFDVSEPTVSRWVRRAPRP
                     PGPARHWLTFLRNHREAIAAMDLFTVPTLTFGILYCFFIIGHDRRRILHHNVTRNPNA
                     LWVALQVHETWDYGQQPQRFLIFDRDSKFSSEVLSTVKAIGCRPVRTALCSPWQNGVA
                     ERWVGSVRQDLLDHVIILNHRHLKRLLNEYVRYYHEDRTHLGLEKDTPGGRVTASSSS
                     SGHKIISLPRLGGLNHRYAVAA"
     gene            complement(3854..5881)
                     /locus_tag="VFC15_15390"
     CDS             complement(3854..5881)
                     /locus_tag="VFC15_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11590.1"
                     /translation="MTLAKLRCIDFASLVLFALGVLLPPSANAQSGNYTYMNIDYPGA
                     YMTTPTAVNDSGSVVGYYLDSQYGSNHGFLYAGGVYTTIDDPEGTTLPQGINNSGEIT
                     GVIEVSYSETYGFTYVNGVFTTFNYPGTNGATNGQGINNHNEIVGIYSTGGYTGFLDN
                     NGSFSSLSYPGADDTIPHAINDSGKIAGFYTASTVPFGFVYDGSTYTSINYTSGYETF
                     AYGINNAGVVVGYSYLNSPTYEVGFLWQAGQFTIINSTAFPQPAGINNNGQIVGVYFP
                     SNNYHGFLATPGSGPQPLQFFPALPCRVVDTRNPNGEFGGPPISGGSSRSFPISQGGC
                     DIPTTAAAYSLNVTLVPIQHDPVGYLTIWPTGQNQPTVSTMNSLDGRIKADAAIVPGG
                     TGGAVSVYVTNTANVVLDIDGYFEPPSSQTLQFHPLTPCRVADTRKSDFPAGLGTPHL
                     SAGVARDFPVLESTCIPANVNAAAYSFNLTAVPYPQLGDPLGYLEVWPTGYEPPHPVS
                     TLNNPTGTYVANAAIVPAGNGGEITGYASNDTDLVIDVDGYFSLNGAGGLSLYPTTPC
                     RVIDTRKVGSGQPFSGTLNPPVNVVNSPCGVPGTAQGYVFNATVVPSGGLSYLTLWPD
                     GENQPVVSTLNAADGWITSNMAIVPNVNGKIDAYADGLTQLILDISGYFAP"
     gene            complement(6720..6818)
                     /locus_tag="VFC15_15395"
                     /pseudo
     CDS             complement(6720..6818)
                     /locus_tag="VFC15_15395"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(6891..7238)
                     /locus_tag="VFC15_15400"
     CDS             complement(6891..7238)
                     /locus_tag="VFC15_15400"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HZM11591.1"
                     /translation="MKSVADQARTIRLPVHMVEIVNKVFRTSRQLVQELGREPTSEEM
                     AKRMDIPVAKLRKVLKFRQVPISLETPIGEEGDSHLGEFIEDRGAISPADAVINVNLK
                     ERTANVLRTFDPA"
     gene            7285..7422
                     /locus_tag="VFC15_15405"
     CDS             7285..7422
                     /locus_tag="VFC15_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11592.1"
                     /translation="MIDVGGNLGCTGSKSAVVPADGGTRKVALYAVEAPENWFEPTFP
                     F"
     gene            7511..>7819
                     /locus_tag="VFC15_15410"
     CDS             7511..>7819
                     /locus_tag="VFC15_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11593.1"
                     /translation="MGEQQNQPFQLSFNPSLKVDFQGSRVTSDGGLLLVRELDERLGL
                     SELIAEHLTDSRGKNTQLPMADLLRQAVYSRLAGYEDVNDAERLAQDPTFRLMGSERI
                     W"
BASE COUNT         1760 a         2164 c         2216 g         1679 t
ORIGIN      
        1 ggcttgagat gctttcgcag tcgctgctcc aactcagggc cgtaatactg cacccagcgc
       61 cagaccgtgg tgtgatcgac attgagaccg cgctcctcca gcagctcctc aacatcgcgt
      121 agcgacagag aatagcgaag ataccaccgg accgcacaaa gtatgaggcc ggattcggtc
      181 tgccgccatt tgaagatcgc gggtcgggtt cccatttggc gactctttca cgtcaccagc
      241 cagccgccct gaaagttgca acacatccat cgtggcaatg cgtcagtgga cttccacggg
      301 gagaagcggt ccaacgagac gcatgaatcg aagacggacc cggacgcgaa gatggcacgc
      361 aaaggcaagg aagccaagct gagttacaac gggctatccc ccaccagact agcggcggcg
      421 attggtgtag aatttatctt gcttttgaca atccgtaaag ataggtgcga gtggtttgcg
      481 tgctgtactg aatctgtcga aggagcctaa ctaccagttc tgatgcgatt gctcggcgta
      541 ttgctctgct ataacgacgg cgactttctg gaagactcca ttcgctacct gctggaacaa
      601 aaccaccact taatagtatg ggatcacgga tctacggacg agaccgcgga catcatccgg
      661 caatacaaaa acgacttact ggaatcgcgc tatgttcccc gggagttcga tttctacgaa
      721 ctctatcccg cgatgtctcg ccatttgacc gacaactata gttccatcta cgattggatc
      781 tcatggcctg atcaggatga gattctggag gggccgtccc gctccaagcc ttaccgcgaa
      841 tggcttgagg acgtacatgc ctcatcctgc aattggatac aattcaataa ctataattac
      901 tggttcacat cttccgacga cacggcaatc aagtcgccgg gagctcgcat ccggcattat
      961 tctatctttc ccgattgtgc gccgaggatt cgcgcctgga gagcgtccgc caccaatatc
     1021 cgcattttca atcacaaccc tccgctgggc gatccctggc cagctcgctt taatctgcgc
     1081 cattattcca tgcgctcgcc ccagcaaatg ttagctcgcg tacttaagga cagatccaac
     1141 ctgcgtcgcg gcggtgacaa ctatcactac gacaacatga agctcaacct ggaccgtctc
     1201 accatcagtc ccgatcagct tcactacgac gacggacgtt cagacctcga tccctcgcct
     1261 gtctttaact ggcgctctat ttatggctcc ggtccaccgc cggatcccgt tccctgatca
     1321 agctatcgat ccagccactg acggccgcga aagtgttagg aatattttcg ggcatttgtt
     1381 ctgggtggtc tttgtagtag tcaaaatcca aaggcgtata ttccgccaca tattcggcat
     1441 aattccggac aagaaggtcg gaaccaggat ccagccggct taccaggcac agtccagtag
     1501 gaggcgtcct tatcgtgacc atgcgcaaat cgggccgatg gactttgaga cacatgggta
     1561 atttccagac gtcgccacta tagaagtgag tagtgcgaac cctttcagag gttatacggt
     1621 cgagcggtaa gcagtcgtgc agaatgataa tcgagctact gccggcaagc ctctccaggt
     1681 gaatgaaatc ccacaggact tgctcgaaca gatggagacc gtcgatgaag gctaaagaaa
     1741 agtggggagt accaagaagg tcacaggggt tataagactc gaagaaagcg tcgctcgtaa
     1801 tcggaagtac gtgagtacgc ggtggaagag gcccggccag ggcgggcaac ggatcgatgg
     1861 ctatgcaagt cgtatcgggc agcgctgcgc gcaaggaacc tccctgccga actccaatct
     1921 cgacgtagtt agcaggcctg aggatctcgt gaatccaacg caacacggtg taataatcag
     1981 ggccgggaag cgcctcggag gcctgctgca aatggatctt tagaagttcg tcatcgtatt
     2041 cagaagaagg caggaccacc cccatactat agcctgtcgt tcatcagaat atcgtacacc
     2101 cgcaatatca atgagttggc catgaggcgg tttggcgacg tcggataaga ttcttgccat
     2161 gatcgtgtgg ctccggcact tcctcggctg gcttcgcggt gccttttccg cccgcgagga
     2221 tctcatcctc gaaaatcttg ccctgcgtca gcaggtgctc gctctccacg cccaacgacc
     2281 tcgccgacga ctcaccgcgt cgcacaagct tttctgggtg gtattgcgaa aactctgggc
     2341 tcgctggaaa cgacctctca tcctggtcac acccagaaca gttgtgggct ggcatcgggc
     2401 tggctttcgg ctgtattgga agtggctttc gagacccaga agtgccggag gacgaaagcg
     2461 cattagtgag gaaattcgca ctctgatctt ccgcatggta gccgagaatc ccacctgggg
     2521 agcaccaggc attcacggtg agctgctcaa gctggacttc gatgtgtcgg aaccaacggt
     2581 ttcccgctgg gtgcggcgag cgccgagacc tccaggtccg gcgaggcatt ggctgacgtt
     2641 cttgcggaat caccgcgagg ccatcgccgc gatggacctc ttcactgtac ccaccctcac
     2701 attcggcatt ctctattgct ttttcatcat cggtcacgac cgacgtcgaa tccttcacca
     2761 caacgtgacc cgtaatccca acgcgctttg ggttgcgctg caagtacacg aaacgtggga
     2821 ttatggccag cagccgcagc gcttcctgat cttcgatcgc gactcgaagt tcagttccga
     2881 agtgctttca accgtaaagg caatcggatg ccggccggtg cgcaccgcat tgtgcagtcc
     2941 atggcagaac ggggtggccg agcgctgggt cggcagtgtg cgtcaggatt tacttgacca
     3001 tgtgatcatt ctcaaccatc ggcacctgaa acggctgctg aacgagtacg ttcgctatta
     3061 ccacgaggac cggactcatc tcggacttga gaaagatact cccggtggtc gagtgacagc
     3121 gtcatcgtct tcgagtggcc acaagatcat ctctctgccg cgactcggtg gtctgaatca
     3181 ccgctacgca gtcgcggcct aacaaatctg cagctatgaa ctagactcgt tcgctgtagg
     3241 gatcagaggg agattcacgc ctggatcctt gcagtgcagc tttccccact cgtctccggg
     3301 acgctcacgc tggtcgaaga gcacgctatg caacgccttc ctgtctaaga aagacgcctc
     3361 ttgcatcgaa agctcggcgg ggcttgtttt gatgaaccac aggggtggac gtatacctca
     3421 ctgcatgact tttgcgctga tggacctcct tgtagcgacg cagaatactc ggagggcggc
     3481 ataattatgg atgcgaacgg taatctctat ggcacgacaa catacggggg ggctatccta
     3541 acttcggcgt cgtcttcgag attacgccgt aggataacgc acctccgagg ccggtatcct
     3601 tcgctagggt gctggcccac atgacgcctc caagctgatc gggtatccga acaagctacg
     3661 attcaagtct gcccagctct acgcctttta gtgagctttc tcctaatagg acggtcccgt
     3721 ctttgcgggg ttggttgtgt atcggctgtt cccgacaagt aatctggcat tgcccaaaga
     3781 gctgaccatc gtcgcatcat gtgatgagcg ccacagcaac tgctgggagt aggcacgcgc
     3841 gagtctcctg tttctacggg gcgaagtagc cggagatgtc caagataagc tgtgtcaagc
     3901 catcagcgta ggcgtcgatc ttgccgttga cgttcggcac tatagccata ttcgacgtga
     3961 tccatccatc ggcggcattc aaggttgaga cgaccggctg attttcgcca tccggccaca
     4021 acgtgagata gctcaaacct cccgaaggaa ctacagtcgc attgaacacg tagccttgtg
     4081 ccgtgccggg cacgccacag ggactgttca ccacattcac cggaggattc agcgtcccgc
     4141 tgaagggctg cccactgcca accttgcgag tatcgatcac gcggcacggc gtggtgggat
     4201 agagtgacaa gcccccggct ccgttcagcg agaagtaccc atcgacatcg atcaccagat
     4261 ccgtgtcgtt gctggcatag cctgtaattt ctccgccatt acccgccgga acaatggccg
     4321 cgttggccac gtaggttcct gtcggattat tcagggtcga caccggatgg ggcggctcat
     4381 aacctgtggg ccacacctcc agatacccta gtggatcgcc caattgtgga taaggaacag
     4441 cagtgagatt gaacgagtaa gcggcggcat ttacgttcgc tggaatacac gtactctcca
     4501 gcacgggaaa gtcgcgggcc actccggccg acaaatgcgg cgtaccaagc cctgctggga
     4561 agtcgctctt gcgcgtgtcg gccacgcggc aaggcgtcag cgggtgaaac tgcaaagtct
     4621 gcgaagaagg tggctcaaag tatccgtcga tgtccaggac gacattcgca gtattggtga
     4681 catagacgct gaccgcgccg ccggttcctc ccggaacgat cgccgcatcg gccttaatgc
     4741 gaccatccaa cgaattcatc gtggagaccg tcggctggtt ctgtcccgtc ggccagatgg
     4801 tcagataccc caccggatca tgctgtatcg gcaccagggt cacgttgagc gagtaagcgg
     4861 cagcggttgt aggaatgtcg cagcctccct gcgagatggg gaaactccgg gaactgcctc
     4921 ccgaaatcgg aggtccgccg aactctccat tcgggttacg cgtgtcgacc acacggcaag
     4981 gcagtgcggg gaaaaactgc aacggctgcg ggccggaacc gggagtagcc aggaatccgt
     5041 ggtagttgtt ggagggaaaa tagacgccga cgatttgccc gttattgttg atgccggcag
     5101 gttgcgggaa cgccgtagag ttgatgatgg tgaattgacc cgcttgccag aggaagccga
     5161 cctcgtaggt gggactgttc aaatagctat atcccacaac cacgccggcg ttgttgatgc
     5221 cgtaagcaaa cgtttcgtag ccgctggtat aattgatgga cgtgtatgta ctgccgtcat
     5281 aaacgaagcc gaaaggaacg gtagaagctg tataaaagcc ggcaatcttg ccgctgtcgt
     5341 tgatggcgtg ggggatggtg tcgtccgctc cgggataact taacgaactg aaggagccgt
     5401 tgttatcgag aaagccggtg tagccgcccg tagaatagat tccgacgatc tcgttgtggt
     5461 tgttgatccc ttgcccgttg gtggccccgt tggtgcctgg atagttgaag gtcgtgaaaa
     5521 cgccattcac gtaggtgaag ccgtacgtct ccgaatagct aacttcgatc actcccgtga
     5581 tctcgccgct attgttaatc ccctgaggaa gggtggtacc ttccggatcg tcaatggtgg
     5641 tgtatacgcc accggcgtaa aggaaaccgt ggttgctgcc gtattgcgag tcaaggtagt
     5701 agcccaccac cgagccgcta tcgttgacgg ccgtgggggt ggtcatatat gcacccggat
     5761 aatcgatgtt catgtaggta tagttgccgc tctgggcatt cgcactgggc ggaagcagca
     5821 cgccgagagc aaagaggacg aggctagcga agtcgataca gcgcagcttg gcgagggtca
     5881 cgtgtcacct tccgacacat cttgattttg cacagaattc aacgacaatg agcgaggtcg
     5941 gtgcgactcc ctgcgtcaga acgggcaacg gtcaaaaaaa gagaaaagac agggttgcta
     6001 taacactatg cgacggctat acgaatcaac ggctaccccg catccagagt tcactcgctg
     6061 cgagattata actcaaagcg cgcagcctcg agcgttgcac aacgattacg attccgtgac
     6121 attggactgc ctggcgaatg aggggcacgg gttgggtcgg agcattgcag gttgcctgag
     6181 agtcgcgggc taagtagccg agagtctatg gcacctgata tatcaccacg ctataaccac
     6241 atgaatatcc aagcggcgct ggaagaagta cgcttcaccg tcaacggcaa agcttgagtg
     6301 caacattgtg atcgagtcac gtgttcccgc caagtcgcgg caacaactta ccgcgatggg
     6361 gcatgatctg gatgtaacaa accagtattc gacctcgatg ggccgcggac aggccgttct
     6421 cgacgatact gcgaccaaaa tacattacgg tgcgtctgat caacgggctg atggcgccgc
     6481 tgaaccggat gccgtgatga gttggtggtg cgagaccagt gcagcgcgtt ggcagagcag
     6541 ggggatctga cgtcagccat gattcacttt cgaaacagga cggcggaagg aaatattcaa
     6601 cccgctagat taggcaggta ccgacctcgc acgctacgta agtgaccatc tcccacggac
     6661 gcggcgttcc tcgacttccg ctcccagcgt gcacagattc cgggggcatt cgtgaacgtc
     6721 tattgaaatg atttgagctt gacagagcgc gaggagtggc gcagcttgtg cagagccttg
     6781 gcttcgatct gccgaatgcg ttcgcgggta acggcgaagt gctggccact tcctccagcg
     6841 tatgctccga accgtcttcc agcccgaagc gcatcttgat gaccttctcc tcacgcgggg
     6901 tcaaaagtgc gcagcacatt cgcggtccgc tctttgaggt tcacgttgat gacggcatcg
     6961 gccggcgaga tcgcgccacg gtcctcgatg aattcgccca ggtgggaatc cccctcctca
     7021 ccgattggcg tttccagcga gattggtacc tgcctgaact tgagtacctt gcgcaacttg
     7081 gccacgggaa tgtccatgcg cttggccatc tcttcggacg tgggttcgcg gcctagttcc
     7141 tgaaccagtt gccgcgaagt acgaaatacc ttattgacga tctcgaccat gtgcactggc
     7201 aggcggatgg tgcgcgcctg atcggcaacc gatttcaacg ggaacatcct ggcgacgcat
     7261 aacccgcatt ttggcgggat ctgtgtgatc gacgtgggcg gtaacctggg ttgcacggga
     7321 agcaagtcgg cagtggtccc ggcggacggc ggcacacgca aggtggcgct ctatgctgtc
     7381 gaagctccgg agaactggtt tgagcccaca tttccttttt gaagttggcc taaaccgcca
     7441 tatgatcggg ccaacgccag tattcatgcg gcgttgacgg cgcaaagtac acaccggagg
     7501 cttgcaccca gtgggtgaac aacaaaacca gccctttcag ctctctttca acccctcttt
     7561 gaaggtagat ttccaaggat cgcgagtaac gtcggatggt ggcctgctgc tggtacggga
     7621 gttagacgaa cgcttggggt tgagcgaact catcgcagaa cacttgaccg actcacgagg
     7681 gaagaacacg caattgccga tggccgatct gttgcgtcag gctgtgtaca gccgattggc
     7741 tggatacgaa gacgtgaacg atgccgaacg tcttgcacaa gatccgacct tccggctcat
     7801 gggctcggaa agaatctgg
//
LOCUS       DASRKK010000634         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_253523, whole genome shotgun sequence.
ACCESSION   DASRKK010000634 DASRKK010000000
VERSION     DASRKK010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253523"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..576)
                     /locus_tag="VFC15_15415"
     CDS             complement(208..576)
                     /locus_tag="VFC15_15415"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM11594.1"
                     /translation="MKRGFTVVVICVVIGLSQALVLHAQAPAAPGAGHGFLIDKHVAA
                     GLTCAKCHIESTAKAPETATCLACHGGTYAKLAAMTQGDQPNPHASHRGEVACAECHH
                     VHKASVTLCNQCHTYDMTTP"
     gene            complement(849..>2479)
                     /locus_tag="VFC15_15420"
     CDS             complement(849..>2479)
                     /locus_tag="VFC15_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM11595.1"
                     /translation="LDWATHLAFSPGQQLQLVNKATRKAVRFSNYAFRYASESGKANR
                     CIEPLPHDRRFVGEDWQRWPYNFMYQAFLLNQQWWHNATSGIRGVTKQHENMVQFVSR
                     QILDMFSPSNFLLTNPEVLRHTVSKGGMNLVSGFQNLIEDWDRRISGKKPVGTEDFVV
                     GRNVAVTPGKVIYRNRLIELIQYAPATDKVRPEPVLIVPAWIMKYYILDLSPQNSLVK
                     HLTQQGFTVFMISWKNPSPEDRDLGLDNYRTLGVMAALDTIRAIVPNQKVHTVGYCLG
                     GTLLSIAAAAMARDGDDRLKSITLLAAQTDFTEAGELMLFINESQLAFLEDMMWEQGF
                     LDTTQMAGAFQMLRSHDLLWSGMIREYLMGERDPMIDLMAWNADTTRLPYRMHSEYLR
                     RLFLNNDLAEGRLIVEGKPIALTDIRAPIFTVGTVHDHVAPWRSTYKISLQTETEVTY
                     LLTSGGHNAGVISEPGHDGHSFQVMTKKANDHYVDPDAYLAAAPRKDGSWWPEWVAWL
                     ATRSGAASAPPSMGAAAAGYAPLCDAPGTYVFQE"
BASE COUNT          510 a          705 c          729 g          535 t
ORIGIN      
        1 gcgctcggcg gcgcgcaagg cgtgcaggcc gtgcacagcc acctcaagac cgagctgggc
       61 atggtgatgc gtctcgccgg cacgcccaat atcaagagca ttacgcggga ttttgtcgcc
      121 cgcgccgagg gtgaagcggg ctgatgctag atacgccgat cggcgcggca gcgaacgttc
      181 aacttccgcg ccggtccggc aaccgcatca gggcgtggtc atgtcgtagg tgtggcattg
      241 attgcacagt gtcaccgacg ccttatgcac gtgatggcac tccgcgcacg cgacttcacc
      301 acggtgcgag gcgtgcggat tgggctgatc gccctgtgtc atcgccgcca gcttggcata
      361 ggtgccgcca tgacaggcga gacaggtcgc cgtctccggc gcttttgccg tactttctat
      421 atgacacttc gcgcaggtca ggcccgcggc aacgtgcttg tcgattagaa acccgtggcc
      481 ggccccgggc gccgccggcg cctgcgcgtg caggactagg gcctgcgaca gaccgatcac
      541 cacgcaaatg acaacgacgg taaagccacg tttcattcgt tacctggctg atggattttg
      601 gctaatagca tccgtctctt caatttgtct agtccataga ccggtgtgtg taccgagaac
      661 tttatctgta ttgattaggg cccgctgtgt gtaccgaaaa cctaaacccg aacgtaacgg
      721 tgatgaagtc cgcttctcta atcggcccat ttcttgtttt agatctgcgg gccgcctgcg
      781 aaattggcgc gttcgtcaag aggtcactga gccatcccta aagcagagca gcagcctgca
      841 acatggcgtc actcttgaaa cacataagtt ccgggggcat cgcagagtgg ggcataaccc
      901 gcggcggccg cgcccattga aggcggcgcg cttgcggcac cggaacgcgt tgcgagccaa
      961 gcaacccatt ccggccacca ggagccatcc ttgcgcggtg ccgccgccaa gtaggcgtcc
     1021 ggatcgacgt agtgatcatt cgccttctta gtcatcacct ggaagctgtg accatcatgg
     1081 ccgggctcgg atatgacccc ggcattgtgg ccgccgctcg tgagcagata tgtcacttca
     1141 gtctcggtct gaagactaat cttgtatgtt gatcgccacg gtgcaacgtg gtcgtgcact
     1201 gtgcccactg tgaagattgg ggcacgaatg tcggtgaggg cgattggttt tccttcgacg
     1261 atcaatcgcc cttcggcaag atcgttatta aggaacaggc gccgcagata ttccgaatgc
     1321 atacgatagg gcaggcgggt cgtatccgcg ttccacgcca tgaggtcaat catgggatcg
     1381 cgctcgccca tcaagtactc gcggatcatt ccggaccaca agaggtcatg ggaacgcagc
     1441 atctggaagg cgcccgccat ctgagtggtg tcgaggaagc cctgttccca catcatatct
     1501 tcgagaaacg cgagctggct ctcgtttatg aagagcatca gctcccctgc ctctgtgaag
     1561 tcggtctgag ctgccagcaa tgtgattgat ttcaggcggt catcgccgtc gcgcgccatt
     1621 gccgcagcag caatcgacag tagcgtgccg ccgaggcaat agccgacggt atgtactttt
     1681 tggttcggca cgatcgcacg gatcgtatcg agcgccgcca tgacacccag cgtccgatag
     1741 ttgtccaggc cgaggtcacg gtcctctgga ctaggattct tccacgaaat catgaaaacc
     1801 gtaaagcctt gctgggtaag gtgcttcacc agcgagttct gcggcgaaag atcgagaatg
     1861 taatatttca tgatccatgc gggtacgatg agcacgggtt cgggccggac cttgtcggtc
     1921 gcgggcgcat attggatgag ctcgattaat cgattgcgat aaatgacctt gcccggagtt
     1981 accgcgacat tgcgaccaac aacgaagtcc tcggtgccga ctggcttctt gccgctgatc
     2041 ctgcggtccc agtcctcgat caggttctga aaaccactca ccagattcat cccgcctttg
     2101 ctgacggtat ggcgaaggac ttcgggattt gtcagcagga aattcgaggg ggagaacatg
     2161 tcgaggatct ggcgcgacac gaactggacc atgttttcat gctgcttcgt aacgccgcgt
     2221 atgccggacg tcgcgttgtg ccaccattgc tgattgagca ggaaggcctg atacatgaaa
     2281 ttgtatggcc atctctgcca atcctcgccg acgaaccgac gatcgtgagg caatggctcg
     2341 atgcagcggt ttgccttgcc gctttcagac gcgtagcgaa aagcatagtt gctgaagcgg
     2401 actgctttcc ttgtcgcctt attaacgagc tgcagctgct ggcccggcga aaaagcaaga
     2461 tgggtggccc aatcaagat
//
LOCUS       DASRKK010000635        31047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_450502, whole genome shotgun sequence.
ACCESSION   DASRKK010000635 DASRKK010000000
VERSION     DASRKK010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 31047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450502"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFC15_15425"
     CDS             complement(<1..198)
                     /locus_tag="VFC15_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11596.1"
                     /translation="MASLQAYQSHGIRYYRVVESFRKDGKPCLRVLAHLGRVDDILRL
                     HQQHQEVPVKLASVSAGAVTAL"
     gene            complement(348..512)
                     /locus_tag="VFC15_15430"
     CDS             complement(348..512)
                     /locus_tag="VFC15_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11597.1"
                     /translation="MSSSKDLVFAITEDIGTDISYVRREHGEKGANYFTGIEIGTLFG
                     GTILAGVSRD"
     gene            complement(611..805)
                     /locus_tag="VFC15_15435"
     CDS             complement(611..805)
                     /locus_tag="VFC15_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11598.1"
                     /translation="MGAAERSLEHALKRQSIEYKTIPGEAAFYGPKIDIKLVDAIGRL
                     WQNIQGLQSMRTTPDESYSC"
     gene            complement(942..1745)
                     /locus_tag="VFC15_15440"
     CDS             complement(942..1745)
                     /locus_tag="VFC15_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM11599.1"
                     /translation="MTNYTLYWRSFRGLLLRDISVLRREVIPFLIRTVMNPLMFVFVF
                     TYLFPKMGQGFQSSATAGGFGTLILPGLLAVGIFIQGIMATALPLSMEIGATREIHDR
                     VMSPLPVTWVAIEKIVFSAVQSMLAALVIFPLVYFIPATPVQVHVSSWFTLIGILVLA
                     SLVAGTVGLAIGTIVKPEQIALMFGIIVVPVTFFGCVYYPWAAMYKVRWLQILVLVNP
                     LVYMSEGLRTALTPNVPHMPAWVAFVALLIGLGILGTVGVKGFIKRVLT"
     gene            complement(1738..2691)
                     /locus_tag="VFC15_15445"
     CDS             complement(1738..2691)
                     /locus_tag="VFC15_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM11600.1"
                     /translation="MTSSTEAIVNIQKMAKTYPGKTPVEAVKGIDLAVERGELFGLLG
                     PNGAGKSTTISVCTTRSLPTAGRVRIAGIDVVEHPSEARRYMGVVTQFNTLDRQCTVW
                     ENLYFHCRYFGIPHSEAAKRSAELLDLFKLTERAKSSVRTLSGGLAQRVQIARAIAHN
                     PEVLFLDEPSAGLDPQTRLALWDIIRELRQRGITVVLTTHYMEEADQLCGRVAIIDHG
                     KILVSGTPEELKNSVGAQRVFQLQLQDSSADGLRARLGTLPGVTSVEASAAGLIVFTD
                     GRGGLLPEIVGITGDNLRDISVSETTLESVFIKLTGRDLRD"
     gene            complement(2754..3920)
                     /locus_tag="VFC15_15450"
     CDS             complement(2754..3920)
                     /locus_tag="VFC15_15450"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM11601.1"
                     /translation="MIHSYDAVVLGGGAAGLMCAITAGQRGRRVAVLERAERIGKKIL
                     ISGGGRCNFTNLHCRPENFLSANPHFCKSALARYTPYDFIALVEKHRIPYHEKTLGQL
                     FCDSSAQDIVGLLEDECRVAGVQIFNATHVREVRRSDNFVVRAGGHEFHAPALVVATG
                     GLSIPKVGATAFGYELARQFGIKIRECHPALVPLTFDADDRKHYCDLAGVSAEVIASV
                     GRQQFREKMLVTHRGLSGPAILQISSYWKPGEALTLDLAPEKDITQRLRLESGGRDLG
                     AAKTAFRAVLPDRLADRWIEQHAPERWTNPALEELERTAHHWSVTPSGTEGYDKAEVT
                     AGGVDTDELSAKTMESGKVPGLYFIGEVVDVTGHLGGFNFQWAWASGFCAGQAI"
     gene            complement(3996..5195)
                     /locus_tag="VFC15_15455"
     CDS             complement(3996..5195)
                     /locus_tag="VFC15_15455"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HZM11602.1"
                     /translation="MPDYNDQPTILISEDQEHVREALAMLLRGQDYLVLTCDSPHEAL
                     TVASQHSPDLALVDMNYRRDSTSGVEGLNLIETLRQLESSLPIIALTAWGNVDLAVHA
                     MRSGASDFIEKPWSNGSLLEKVRSLVSHGREVRSTQRISEHEKQDAQQLQTRIVPRRH
                     LVTGGIELFGDSVPAGIVGGDYFGVWQPNPESLHFCVADVSGKGTPGALIAAMLYASV
                     STLSASGNGAAKIVSEVEAILHDQLGEGHYVTLFYGVLDLTSHVLEYVNAGHCPPIVR
                     RVNGAIELLSPTRPVLGLLDQQAARSERIQLCPGDDLALYTDGITEAADESGEEFGPE
                     RLRNLLHSEGERSLQERHSHILRGVRQFASGKLADDATLVLISVAGTALAQTSTAAKS
                     ACSQTER"
     gene            complement(5345..8584)
                     /locus_tag="VFC15_15460"
     CDS             complement(5345..8584)
                     /locus_tag="VFC15_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM11603.1"
                     /translation="MNVSGPAIERPVATFLLTIALLLAGGLAYTQLPVSPLPQIDFPV
                     ISVGAGLPGASPETMATSVATPLERQFGRIAGINQMTSTSQLGSTGITMQFDLNRNID
                     AAARDVQAAINAARGYLPADLPGNPSYRKVNPADSPILILALTSDTMTQDQMYDAADS
                     ILSQKLAQVAGVGQVYVGGSSQPAVRIEANPNLLNKLNIGLDAVRTAVAAQNVNQAKG
                     SLQGGDKSWMISDNDQLKKAKDYRPIIVAYRNGAPVRLSDVADVEDSYTDTHVAGRFN
                     GKPCVMIIIFRQPGANIIATVDRIIGQLPFLKASMPQNIKLDIALDRTTTVRASVADV
                     ERTLIISLILVVLVVFVFLREVRATFIPSVAVPLAIIGTFGIMYLLNYSLNNLSLMAL
                     TISTGFVVDDAIVVLENVMRHVEAGMAPHEAALLGAREIGFTVVSMSISLIAVFIPIL
                     LMSGLIGRLFHEFAMTISGAIAVSMVVSLTTTPMLCARLLKPRGAKHGFIYRASEKGF
                     IRLNSGYKRSLAWVLRHQRLVFAIAVLAVFVNVYLYIIVPKGFFPQQDTGRMMGTIMA
                     DQDMAFAGMRERVAEFIKIVKSDPAVDNVIAFSGGNTAQNQGRMFVTLKPLNQRKVSI
                     DQVINRIRPKTSHVPGATLFFQAAQDLQIGGRFANAQYQYTLSGEDLAELYAWAPKLT
                     DKLKQIPILKDVSSDQQVRGLQQNVIIDRDTAARLGITPSQIDNVLYDAFGQRQISTI
                     YQTLNQYHVVLEVDPQYQMTPDALQSVYMITRPGAQVPTGIPATVSGSTTLSSGQPAN
                     GGSAATVTPATTATPASTGAQVPLSAFAHFGPTSTSLAVAHQGQFPAVTISFNLAPGA
                     ALGDATKLIEQTQLQMRFPATIHGSFQGTAAAFQDSLSSEPLLILAALLAVYIVLGML
                     YESYIHPITILSTIPSAGTGALLALMLFHMELNVIALVGILLLIGIVKKNAIMMIDFA
                     LEMERQGKTPLDAIHEAALLRFRPIMMTTAAALLGAVPLAFGRGTGSELRRPLGVAIV
                     GGLMVSQMLTIYTTPVVYLYLDRFQTWLVAHLPWRRKQLQKKEVPIFAD"
     gene            complement(8587..11688)
                     /locus_tag="VFC15_15465"
     CDS             complement(8587..11688)
                     /locus_tag="VFC15_15465"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZM11604.1"
                     /translation="MSISRPFILRPIATSLLMVALLLVGIVAYQQLPISALPQVDYPT
                     IQIVTIYPGGSPDVTASSITAPLERQFGQLPGLNQMTSSSSFGASVITLQFNLDENID
                     VAEQEVQAAINSAQTFLPPDLPTPPIYSKVNPADAPILTLAMTSDTMPLPQIEDLADT
                     TFAQKISQLPGVGLVSISGGQKPAVRIQANPTALASYGMSLEQLRTMIAAVNVDQAKG
                     QLQDNRQAYTIGANDQLFKPEDYAKTIIAYKNGNPVRVSDVATVIQGPENLNQAAWMN
                     TTPAVILNIQRQPGANIIKVADSVKLRLKQLQANLPASVKITVLTDRTNTIRASVADV
                     KFSLMLTIALVVLVIFLFLRTLAATIIPSIAVPLSLVGTFGVMYLLGYSLDNLSLMAL
                     TISTGFVVDDAIVMIENIDRYIEDGFSPLEAALRGSGQIGFTIVSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAVTLSVTILLSAFISLTLTPMMCSILLRQKGQEKHGKLYEASERV
                     FNSTIAFYGRTLKVVLEHQTLTLIITLGTLVATIWLYILVPKGFFPIQDTGVIMGISD
                     APESVSFASMAERQVRLAAAILKDPDVASLSSFIGVDGTNTTPNSGRFQINLKPKDER
                     NDDVSAIIKRLQQETAGVAGITLYMQPVQDLSVEDRVSRTQYQFSLEDADPKELAQST
                     STLMAKLKTLPQLRDVATDQQNQAAVANLQIDRDTASRLGVSASAVDNTLYDAFGQRL
                     ISIMFTQLNQYHVVLEVDPSFRDDPDSLKSIYVPGTTGQVPLSAIAHFETGTGALSLN
                     HQGQFPAVTISFNLGGRYSLGEAVQAINGAVAEVGLPPSVQVKFQGTARAFEASQANE
                     GWLILAAIITVYIVLGVLYESYIHPVTILSTLPSAGVGAILALNIFRMDLGVMALIGI
                     ILLIGIVKKNAIMMIDFALEAERKEGKAPLEAIYEACLLRFRPIMMTTFAALFGAVPL
                     ALGSGTGSELRRPLGVTIIGGLLFSQILTLYTTPVVYLWFDRLGAALRNIRAPKPAEA
                     MGD"
     gene            complement(11685..12848)
                     /locus_tag="VFC15_15470"
     CDS             complement(11685..12848)
                     /locus_tag="VFC15_15470"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HZM11605.1"
                     /translation="MTLLRIHKVGIALLLCAVLAGCSRADKNAKASGPPPSVPVGVAT
                     VQQHDFPVYLTGLGAVQAFNTVSLKSRIDGQIMQVNFQEGQDVSVGQLLIQIDPRPYD
                     VALQTAKANLQRDEAQLNNAKVQYARMQALYAGGVIAKQDLDTQEAMFGQYEGTIAAD
                     KAAINSANLNLVYTRITSPINGRIGLRQVDIGNYVQAASSTPMVIITQLHPIAIDFTL
                     PEDQLQAVRQRMKEGTLVVDVYSRDDQTKLSTGKLLTIDNQIDQTTGTAKFKAVFENP
                     DNNLWPNQFVNVHLLLETRKDALTMPASAIQRGPQGTFTYAVDANNTVQVKAVQIALT
                     QGNVVVIESGLQAGDKVVTDGQEKLQPGSRVSVQAPVNQSKSPQPAEGNGLHS"
     gene            13080..13808
                     /locus_tag="VFC15_15475"
     CDS             13080..13808
                     /locus_tag="VFC15_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM11606.1"
                     /translation="MKPVNHLLIIDDDHALVNLLKRFLEAEGFHIDAAYDHDTGLAAA
                     LGDHHELIILDVMLPGGSGFELLKALRHQSAVPVLLLTARGEAVDRILGLEIGADDYL
                     AKPFDPRELVARIRAVLRRTREAASGTPRGDHDEVLNVGDIELSLGTRNVTCGGKPIE
                     LTSVEFNVLELLLRNAGNVVTREQIAEVALGRPLNSFDRSVDVHVSRLRKKLGSLPGN
                     DDLIRPIRGIGYFLASATPEKSKA"
     gene            13805..15214
                     /locus_tag="VFC15_15480"
     CDS             13805..15214
                     /locus_tag="VFC15_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM11607.1"
                     /translation="MNRRLFWKIFLPFWVAQALLLGMLYLRLHYRLHAENPWWVQPER
                     RLVPTLADLAVRRYEEGGQPALRALLDQTAMPHRSDYWLLDAAGRELSGRPLPDEIID
                     FAQRALRHEGMVRPDEAVVLVAPVVTPRSSYLLIGEFIPPPLSERVPGDILWVLKLGT
                     IISALMCLVIAHYLSKPIERLRLATNELARGNLDIRVGATIGKRSDEIADLVRDFDSM
                     AGELRNQIQSERNLLSGVSHELRSPIARMRLALALARTSDRPERDEMLDRIEQDTIQL
                     DSMLERILTVARLENGQYKPKFESLSLNDLIDEVLDDARFEAAATDVTVNYADDAMLH
                     VNGDPGLLRSAIENVVRNAIFYSGQGGRIDVGLRLDDSHAILTVRDNGKGVPDDALAL
                     LFKPFYRVDDARGTTTGGMGLGLAIVRNAVAVHGGTVTARNVSPHGLEVELRLPVAGS
                     ALPAGNSAAQMVAPLQGRR"
     gene            complement(15307..15981)
                     /locus_tag="VFC15_15485"
     CDS             complement(15307..15981)
                     /locus_tag="VFC15_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HZM11608.1"
                     /translation="MLIPSIDLMGGRIVQLVQGEKKALEFDNFGEWIERFSRYPLVQL
                     IDLDAAMGNGSNQALIADICSKLPCQVGGGIRSGENARKVLAAGAKRVILGSSLIKDS
                     GVDTVFARTLCEELGAERLVMAIDSRGGRVAIHGWKTTTAITPAEMMQALEPSCSAFL
                     YTHIDTEGLLQGIPLSVVDGLRRLTSRRLIAAGGIRSQQEIDTLDTMGVDAVVGMAIY
                     SGLLQV"
     gene            complement(15975..16751)
                     /gene="hisF"
                     /locus_tag="VFC15_15490"
     CDS             complement(15975..16751)
                     /gene="hisF"
                     /locus_tag="VFC15_15490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZM11609.1"
                     /translation="MHCETLAKRIIACLDVNAGRVVKGIQFEQIRDAGDPAELALAHS
                     QAGADEIVLLDITATAESRNTLLDTVRRTAKQLFIPFTVGGGIRSLDDAAAVLDCGAD
                     KVAMNSAALAHPTLITQVAERYGSQAVVVAIDARQRTPTAWEVFVWGGRKPTGRDAKQ
                     WAIAAEDLGAGEIMLTSMDRDGTKSGFDCPLTRTIATSVRIPVIASGGAGEPHHFVEV
                     FQAGAADAALAASVFHFGLKSVAAIKQELRDAGIPVRPTC"
     gene            complement(16741..17334)
                     /gene="hisH"
                     /locus_tag="VFC15_15495"
     CDS             complement(16741..17334)
                     /gene="hisH"
                     /locus_tag="VFC15_15495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZM11610.1"
                     /translation="MIAIVDYGAGNLTSVVKALQHLGKECAVTNHPDIVGRADKWIIP
                     GVGNFKATAPLVNGKLGQVLREAASPEGRPLLGICLGLQWMFEGSSEAPELRGLATFA
                     GECTRFSDLVKSPHVGWDSIAIDSRSRLLAGIASGAYVYFTHSYRAPVCEDTVATCTY
                     EEPFTAAVERGNLFGVQFHPEKSGDVGLHILENFCAL"
     gene            complement(17331..17918)
                     /gene="hisB"
                     /locus_tag="VFC15_15500"
     CDS             complement(17331..17918)
                     /gene="hisB"
                     /locus_tag="VFC15_15500"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZM11611.1"
                     /translation="MRRARIHRKTNETDIRIALTIEGHGRAKVSTGIRFLDHMMELVV
                     RHGAFDLELQAAGDLDVDQHHTVEDVGIALGEAFTAALGSKRGILRAGYFVMPMDETL
                     GLAAVDFGGRSAAVVDTKVRSRLVGDLQAELVADFFDGFSRGAKANVHVKTLYGRSNH
                     HKIEALFKAFARALRVACSKDKQLGRMLPSTKGLL"
     gene            complement(17915..18991)
                     /gene="hisC"
                     /locus_tag="VFC15_15505"
     CDS             complement(17915..18991)
                     /gene="hisC"
                     /locus_tag="VFC15_15505"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZM11612.1"
                     /translation="MLSARKAVQQMHEYHPPLAGRKGLRLDFNENTEGCSPRVLARIR
                     EITAEDLARYPEREPVEAIAAAYLGLPAEQVLLTNGVDEGIHLLCEAYLEPSDEVIVV
                     TPTFSMYEIFAEATGATVVRVQCDEDLRFPVEKVLAKISGATKLIAVASPNNPTGGVA
                     SREQLLAIAKNAPGAALLVDEAYYDFYGKTVIADVGTQPNLFVARTFSKAYGLAGLRI
                     GILAGPREQMPMVRRVSSPYSVNAVALLALPVALADSKFVAAYVLQVKAGRAQLENDL
                     SRAGIAWWPSEANFVLMQIGKRHCDFVAAMRQRGILVRDRSSDPGCDGCVRITLGTSE
                     QTERLLVALRDVVPELKLGAGVSA"
     gene            complement(18984..20228)
                     /gene="hisD"
                     /locus_tag="VFC15_15510"
     CDS             complement(18984..20228)
                     /gene="hisD"
                     /locus_tag="VFC15_15510"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZM11613.1"
                     /translation="MRILEGKAAERAVAKLEGRASRLEEVEPNVRKIIQGVRKGGDKA
                     LVRYATLWDKLEKGKPIRVPQQEMEQAWETTPADTRDALKRAAANIRRFANWQMPKSW
                     RKNISGGELGQVVRPLASVGCYVPGGRYPLPSTLLMTAIPAQVAGVERIVVVTPNPQP
                     ATLAAARLLWITEVYRCGGAQGIAALAYGTESIARVDKIVGPGNAFVTAAKKLVSFEC
                     AIDMLAGPTEAVVLSDNGDTEFIASDLVSQAEHDPDAVVVFVSTKKDLAEDVTQNVER
                     QAKGNAIAQQSVRRNAYALVASTREQAIDWVNRIAPEHLTVDDDSDLMAVRNAGSVFV
                     GNYSPQSAGDYAAGPNHVLPTAGAARFRGGLSVADFVKIITVQKFTSEGLREIASVVT
                     KLAETEGLRAHAESVRVRSGNA"
     gene            complement(20228..21097)
                     /gene="hisG"
                     /locus_tag="VFC15_15515"
     CDS             complement(20228..21097)
                     /gene="hisG"
                     /locus_tag="VFC15_15515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZM11614.1"
                     /translation="MKLRLGLPKGSLQEATLALLARAGLQVYVSPRSYFAGTNDPEIE
                     CMLIRAQEMTRYVEHGALDAGMTGLDWVTESGLEVVTVADLIYAKQSRGKVRWVLAVP
                     EDSPAQRAEDLRGATIATELVNVTTRYFLQRGIDVKVEFSWGATEVKPPTLADAIVEV
                     TETGSSLKANRLRIIETLLESNTQIIANKKAWTDAGKRQKIENLALMLRGAMEAQGRV
                     GLMLNVRVEDLQAVLAVLPALNSPTISTLSDKAWVAVNTIMEESLCWEVIPRLKAANA
                     TGIVEYPLNKVVM"
     gene            complement(21094..21444)
                     /gene="hisI"
                     /locus_tag="VFC15_15520"
     CDS             complement(21094..21444)
                     /gene="hisI"
                     /locus_tag="VFC15_15520"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZM11615.1"
                     /translation="MPVEIDFDKMMGLAPAIVQDAATGEVLMLGFMNRAAYDKTLATG
                     YVTFYSRTRNELWTKGETSGNRLKVISALADCDLDTVLCRVEVEGQGVVCHTGTRTCF
                     NTELLVATATGGGR"
     gene            21733..22575
                     /locus_tag="VFC15_15525"
     CDS             21733..22575
                     /locus_tag="VFC15_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM11616.1"
                     /translation="MFTSDLLKNKRFLVTGGGTGIGRALTERFLQLGATGYVCGRRAE
                     VIQQTATELAASTGSAIESFVCDVRYSDDVDKVIEQIWAKGPLDILVNNAAGNFLAKT
                     ETLSSHAFEAVIGIVLNGTINMTLACGKRWLAAKHPGMVLNIATTYADTGSAYVVPSA
                     VAKAGVVALTRSLAVEWGGRGVRFAAIAPGAIRTEGAFSRLLPVPEMEKALLDHNPLH
                     RLGTLEEISNLAAFLVSDQAGYINGEMVYMDGGEMLAGASVFSLLGRKVPDVAWEMLK
                     PRKK"
     gene            complement(22636..23487)
                     /locus_tag="VFC15_15530"
     CDS             complement(22636..23487)
                     /locus_tag="VFC15_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11617.1"
                     /translation="MKARVLYPALALIVMVATLSMTATASDPNPMGTTDSSTELSVPA
                     PDATNSSQSQTPGQPAPKPQSADQDAHKVGIGFVFGTLGLGGQGAVGLNRKLDVRGEL
                     NFFRISRSSVRNGITYDASIHLGNVGVLLDWFPWANGFHLSPGLLVYNGNKITGSASV
                     TGGQFFTLNGAPYFSSTTTPVTGSATIGLNKVAPEILFGWGTLLPRSRHWSVIFDAGV
                     LFWGSPNVTMNLTGDVCDFGQTNCRAISSDPTVQANIKAQEAKYNSDASPYKVFPVIQ
                     FGVGYRF"
     gene            complement(23714..24844)
                     /gene="asnA"
                     /locus_tag="VFC15_15535"
     CDS             complement(23714..24844)
                     /gene="asnA"
                     /locus_tag="VFC15_15535"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HZM11618.1"
                     /translation="MADKKADLAGPGIGDYLELEKVLPDNYSSLLTPKETQRALFEVK
                     RYIEDNLCKELNLMMVEVPLIVDEESGVNDMLDRDGSRTPIGFHISNDRDKHPINAQV
                     VQAATKWKRMALKQFGMAAGEGLCTDMRAVRKDYFLDHDHSAYVDQWDWERVITPEQR
                     NLDFLKGIVTKIWKVIVGAERYAQELFQALCDPKYPNLPEQITFLHAEEILDMYPDLP
                     RKQRETAILQKYPAVFIIGIGWMLRDGYPHEMRAADYDDWVTPTITKNGHMMHGLNGD
                     ILVWNPVTHRRHELTSMGIRVNAESLKKQLELTHQMDFLKYPYHQAIVNHEIPLSIGG
                     GIGQSRTMMLILRKAHLGEVSVTVWPKVLKEMCEKKNIHVLN"
     gene            25114..27096
                     /gene="glgB"
                     /locus_tag="VFC15_15540"
     CDS             25114..27096
                     /gene="glgB"
                     /locus_tag="VFC15_15540"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZM11619.1"
                     /translation="MAPSDPKKQVPAKEEAPAIEPAPAPEQGEDQGSVLLTSYDLYLF
                     NEGKHNRLYHKLGAHLTEIDGYPGTYFAVWAPDAERLSVVGDFNGWNSDSNPMTPRGD
                     SGVWECFVRGLGKGSIYKYHLRSRFHMYQVNKADPYGFHHETPPRTGSIVWDLDYQWG
                     DEDWMSLRHKRNAFDAPMSVYEVHIGSWMRNQEQGNRWFSYRELAPRLAAYVNELGFT
                     HVEFMPVMEHPYFASWGYQVTGYFAPSSRFGTPQDFMYLVDYLHQRGIGVILDWVPSH
                     FPNDQHGLAYFDGTHLYEHADPRKGFHPDWKSAIFNYGRNEVRGFLISNAMFWLDEYH
                     IDGLRMDAIASMLYLDYSRKRGEWIPNMYGGRENLDAVSLLQRVNHDAYQEFPDIVTV
                     AEESTAWPMVSRPTYIGGLGFGMKWDMGWMHDTLAYFQQDPIHRKYHHNKLTFRGLYA
                     FTENFTLPLSHDEVVHGKGSLIGKMAGDYWQKFANLRLLFGYMFTQPGKKLIFMGGEI
                     GQWSEWNHDGSVDWILLTFPPHQGIKRWVSDLNAFYRGEPAMYEYDFNPAGWRWIDAN
                     DNDNSVLTYLRYGKDENAPLVVACNFTPVPRRGYRIGVPKPGHWREVLNSNATIYGGT
                     DEGNYGGANSEAIASHGFEQSLLLNLPPLGMVVFKQ"
     gene            27289..27507
                     /locus_tag="VFC15_15545"
     CDS             27289..27507
                     /locus_tag="VFC15_15545"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZM11620.1"
                     /translation="MATNLAIDDKLIEEARRIGGHVTKKDAVTAALDEYIRRRKQVDV
                     LKLFGTVEYERGYDHKASRRRDRIEHDR"
     gene            27504..27914
                     /locus_tag="VFC15_15550"
     CDS             27504..27914
                     /locus_tag="VFC15_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM11621.1"
                     /translation="MKLLADTSVWSLALRRRATATFSSEDIRLKAALAEAISDGRVVM
                     IGPIRQELLSGIKEQSQFEKLRTALEAFRDEAINTPDYDDAARFYNLCRSSGVECGPV
                     DILICAVAVRRKWSVLANDALMNRCLQIIVAHRP"
     gene            28266..29072
                     /locus_tag="VFC15_15555"
     CDS             28266..29072
                     /locus_tag="VFC15_15555"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZM11622.1"
                     /translation="MAPSKDSDQEHGKEHLAMKTPPIVSTQEWENARQQLLLKEKAFT
                     RSRDALAAERRRMPWVAVDKKYEFDGPKGKVSLLDLFEGRPQLILYRAFFEPGVFGWP
                     EHACRGCSFGADQVAHLAHLNARDTTLAYTSRAPQADIARLKARMGWEIPWYTMTDSF
                     DVDFGVDEWHGHNVFFRDGERVFRTYFINARGDEAMGTTWSYLDITPLGRQETWEDSP
                     EGYPQTPPYKWWNWHDNYDAEASPDPKWVDITTDPKGAAARRRDAEVKAT"
     gene            29151..29294
                     /locus_tag="VFC15_15560"
     CDS             29151..29294
                     /locus_tag="VFC15_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11623.1"
                     /translation="MMTKHRRGDSEVERDLQDNENSGPVDILICAVAVRRKWSLLAND
                     ARS"
     gene            complement(29824..>31047)
                     /locus_tag="VFC15_15565"
     CDS             complement(29824..>31047)
                     /locus_tag="VFC15_15565"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZM11624.1"
                     /translation="FLEKTNHRLGGSALNRVCSIFSQILQLFPRLEFESAVRKPKAER
                     HARGFTSWGQFVAMLFCQLGHAQSLREICGGLAASEGKLKHLGLPAAPARSTLAYANE
                     HRPWPLYQTVFQQLLEKCQSVVASQPGKTKRKFRFKNKLLSLDATVISLCVSVFDWAQ
                     FRRTKGAVKLHLLLDHDGHLPSYAVLTEGKQHEVRVARQLQFAPGTVWVIDRGYTDYE
                     WFARLTQDGVYLVTRLKDNADYAVVEEREIPQRKGVRRDQVIFFYKLAQAGVECFFRR
                     VEYYDEEQDQILVFLTNHMKLAAATIAAVYKDRWAIELFFKALKQNLRVKTFVGTSEN
                     ALQTQIWTALIAMLLIKYLQLRSRFGWSLSNLVALLRQQLFVYRDLWSWIDSPFQAPP
                     LLAPGFEQLRLGLEQ"
BASE COUNT         6255 a         9726 c         8797 g         6269 t
ORIGIN      
        1 cagtgcagtg accgcaccgg ccgacaccga cgcaagcttc accggcactt cctggtgctg
       61 ctgatgaagt cgcagaatgt cgtcgacccg gccgaggtgg gcgagcacgc gcaggcaggg
      121 cttgccgtcc ttgcggaagg attcgacgac ccggtagtag cggatgccgt gcgactgata
      181 ggcctggagc gaagccattt agctacgtat atacataaac cggatactag ttgtcaacaa
      241 ccgatattag ctgtcatata tggagtaaac cgccgataca tttccgtata tacaacagag
      301 cgcgcttcaa tccttttcaa atcaatcact tcaaatagag atgctggcta gtcgcgggaa
      361 actcccgcta ggatcgtgcc gccaaacagg gtgccgatct cgatgccggt gaaataattg
      421 gcccctttct caccgtgctc gcgcctgacg tatgaaatat ccgtcccgat atcctccgtg
      481 atggcaaata ccagatcttt gctgctgctc atgtcgcctc ctgtttgcaa ggcccgctgg
      541 tctcccggca ttcacactgg ctgcggcgaa tttgtcgcca agtacatggt caatcgtaga
      601 tctgcgacag tcaacaacta tatgactcat cgggtgttgt acgcatcgac tgcaagccct
      661 gaatattctg ccagaggcgt ccgatggcgt cgaccagctt gatgtcgatt ttggggccgt
      721 aaaacgcggc ttcgccgggg atggtcttgt actcgatgct ctgccgcttg agcgcgtgtt
      781 cgagcgaacg ctcggccgcc cccattgatc ggggcgcccg gcgtaggtct tctggtcgtt
      841 cgggtcccag gtggaaagct cgacctgata tttgtcgaac ccgtagacgc cttgtgcccc
      901 gggttcgcgc ccgttttttt gggcgctagc ctgggaccct tctacgtcag cacccgcttg
      961 ataaaccctt ttaccccgac cgtcccgagt atccccaggc cgatcagcag cgccacaaac
     1021 gcgacccacg ccggcatgtg cgggacgttc ggtgtgaggg cggtgcgcag cccttcgctc
     1081 atgtacacca acggattgac cagcaccagg atctgcagcc agcgcacctt gtacatcgcg
     1141 gcccagggat aatacacgca gccaaagaac gtcaccggca ccacgatgat gccaaacatg
     1201 agcgcgatct gctccggctt cactatcgtc ccaatcgcca ggccgacggt tcccgcgacc
     1261 agcgaagcca gcaccaggat cccgatcagc gtgaaccaac tcgacacgtg gacctgcacg
     1321 ggcgtggcgg gaatgaagta cacaagcggg aaaatcacca gcgcagccag catgctctgc
     1381 accgcgctga acacgatttt ttcgatcgcc acccaggtca cgggcagagg cgacatcacg
     1441 cggtcatgga tttcgcgcgt cgcgccgatc tccatcgaca gcggcaacgc cgtcgccatg
     1501 atgccctgaa taaagatgcc caccgccagc agtcccggaa ggatcagcgt gccgaagcct
     1561 ccggcggtgg cgctgctctg gaagccttgt cccatcttgg ggaacaggta ggtgaacacg
     1621 aagacgaaca tcagcgggtt catcacggtg cggataagga acggaatcac ctcgcgccgc
     1681 agcacgctga tgtcgcgcaa caacagccct cggaacgagc gccaatagag cgtgtagtta
     1741 gtcacgcaag tccctccccg tgagcttgat gaagaccgat tccagcgtag tttcactcac
     1801 tgatatgtcg cgcagattgt ctcccgtgat gccgacgatc tccggtagaa gcccgccgcg
     1861 gccatcggta aatacgatta ggcccgccgc cgatgcttcc accgaagtga cgcccggaag
     1921 cgttcccagg cgcgcgcgca gtccgtcggc gctcgagtcc tgcaactgca actgaaacac
     1981 ccgctgcgcg cccacgctgt tcttcagctc ttcgggagtt ccgctcacca ggatcttccc
     2041 gtggtcgatg atcgcgaccc gcccgcacag ctggtcggct tcttccatat agtgcgtggt
     2101 aagaacgacg gtaatgccgc gctggcgaag ctcgcggatg atgtcccaca atgccagacg
     2161 cgtctgcgga tcgagtcccg cgctgggttc gtcgagaaag agcacttcgg gattgtgcgc
     2221 gatagcgcgc gcaatctgca cccgctgcgc cagaccaccc gatagagtgc ggaccgaaga
     2281 cttcgcgcgt tcggtcagtt tgaacaggtc gagcagttcc gccgacctct tcgccgcttc
     2341 cgagtgcgga attccgaagt accggcagtg gaagtacagg ttctcccaca ctgtgcactg
     2401 ccggtcgagc gtgttgaact gggtcaccac gcccatgtag cgccgggcct ccgacgggtg
     2461 ctcgacaacg tcgatgcccg cgatgcggac ccggcccgcg gtgggcagcg agcgcgtggt
     2521 acacacgctg atggtggtgc tcttaccagc gccattcggc cccagcaagc cgaataactc
     2581 tccgcgctcc accgccaggt cgattccctt caccgcctcg accggcgttt tgcccggata
     2641 ggtcttcgcc atcttctgta tgttgacgat cgcttcagtg gaactggtca cggctctccg
     2701 ctcagtttct gtaggattac cccaaaggaa tgtagcagta gtttatcaag caatcagatc
     2761 gcctggcctg cgcagaatcc agaggcccat gcccattgaa agttgaatcc gccaagatgt
     2821 ccggtgacgt ccaccacttc gccgatgaag tacagccccg gaactttgcc gctctccatg
     2881 gtcttcgccg acagctcgtc tgtgtccact cccccggccg tgacctctgc tttgtcgtag
     2941 ccttcggtgc cggatggcgt cacgctccag tgatgtgcgg tgcgctccag ttcttcgagc
     3001 gcggggttcg tccagcgctc gggtgcatgc tgctcgatcc agcgatcggc cagccgatct
     3061 ggcaggacgg cgcggaatgc cgttttcgcc gcgccgagat cgcgaccgcc ggactccaat
     3121 cgcagccgct gcgtgatgtc cttctcaggc gctaagtcga gggtcagcgc ctcgcccggt
     3181 ttccagtacg acgagatttg caggattgcc ggaccgctga gacctcgatg agtaacgagc
     3241 atcttctcgc gaaattgctg gcgtccgacc gatgcgatca cctcagcgga aacgccagcc
     3301 aggtcgcaat agtgtttgcg atcgtcagcg tcaaaggtca aaggaacaag tgccggatgg
     3361 cattcgcgaa tcttgattcc gaattgtcgt gccagctcat agccaaacgc ggtcgcaccc
     3421 accttcggaa tcgacaaacc gccggtggcg acgaccagtg cgggtgcatg aaattcgtgg
     3481 ccgccagcac gcacgacgaa gttgtcgctg cggcgtactt cccgcacatg ggttgcattg
     3541 aatatctgga cgcccgcgac gcgacactca tcttcgagca atccgacgat gtcctgcgcc
     3601 gagctgtcgc agaacaactg gcccagcgtc ttttcgtgat acgggatgcg atgtttctcc
     3661 accagcgcga tgaagtcgta cggcgtataa cgtgccaggg ctgacttgca gaaatggggg
     3721 ttggctgaca ggaagttctc cggccggcaa tgcaggttgg tgaagttgca gcgtccgccg
     3781 ccggagatca gtatcttttt gccaatgcgc tcggcgcgtt ccagcacagc gactcgacgt
     3841 ccacgctggc cggccgtgat ggcgcacatc agcccggccg cgcctccgcc caggaccacc
     3901 gcgtcatagg aatggatcac agaccagttt caagtttcct gtttcgagat tcaagtgcgg
     3961 ggtgcggaaa tctgcgagtt gaccagagat tgccgtcagc gttcggtttg ggagcacgcg
     4021 gacttggccg ccgttgacgt ttgcgccagc gcggtccccg ccaccgagat cagcaccagt
     4081 gtggcatcgt cggccagctt tccgctggcg aattggcgga cgcctcgcag gatgtgcgag
     4141 tgcctttcct gcaacgatcg ctcaccctcg ctgtggagca ggttgcgcaa ccgctccggg
     4201 ccgaactctt ccccgctctc gtctgccgct tcggtgatgc catcggtgta cagcgcaagg
     4261 tcatcgccgg ggcagagttg gatgcgctcc gatcgggcgg cctgctgatc cagcaatccc
     4321 agaaccgggc gcgtgggcga caacagttcg atcgcaccgt taacgcgacg cacgataggc
     4381 gggcaatgtc ccgcattgac gtattccaaa acgtggctcg tgaggtcgag cactccataa
     4441 aacaaggtca cataatggcc ttcgcccaac tgatcgtgga gaatggcttc aacctcgctg
     4501 acgattttcg ccgctccgtt accggacgcc gacaatgtgc tgaccgaggc gtagagcatg
     4561 gcagcgatca gcgcgccagg cgtgcctttg cctgaaacgt cggctacaca gaagtgcagc
     4621 gattccgggt tgggttgcca gacgccgaaa tagtctccgc ccacgattcc ggccggaaca
     4681 ctgtcgccga acaactcgat gcctcctgta accaggtgac gccggggaac gatgcgagtc
     4741 tgcagttgct gtgcgtcctg tttttcgtgc tcggaaatac gctgcgtcga gcgcacctcg
     4801 cggccatggc tgacaagcga ccggaccttc tccaacagcg aaccgttgct ccacggtttc
     4861 tcgatgaagt ccgacgcgcc cgaccgcatc gcatgcaccg cgagatcgac gttgccccat
     4921 gcggtgaggg caatgatagg aagcgagctt tcaagttgcc gcagcgtttc gatcaggttc
     4981 agcccctcca cacccgaggt gctgtcgcga cgatagttca tgtcaaccag cgccaagtcg
     5041 ggggagtgtt ggctggcgac ggtcagcgct tcgtgaggcg agtcgcaagt taacacgagg
     5101 taatcctggc cccgaagcaa cattgcgagc gcttcgcgca catgctcctg gtcttcagaa
     5161 atcagaatcg tgggctggtc gttgtagtcc ggcatagtgg gcctgcaatg ggagatgcag
     5221 acggagtgcc attcttcgtc agcgccaagt gccacggaat caaggggtat tttccgggat
     5281 gcttacgggt gtgcccagct ataagattgg cgtgtccaaa atcggacagc ccgtaatccg
     5341 aatgtcagtc cgcgaaaatc gggacttcct tcttttgcaa ctgtttcctg cgccacggca
     5401 ggtgcgccac gagccaggtc tggaagcggt cgaggtacag gtataccacc ggcgtagtgt
     5461 aaatcgtcag catctggctg accattaggc cgcccacaat cgcgacgccc agcggacgcc
     5521 gcaattccga accagtgccc cggccgaacg ccagcggaac cgctccgagc agcgcagccg
     5581 ccgtggtcat catgatggga cggaagcgca gcagcgcagc ttcgtggatg gcgtcgagcg
     5641 gggtcttgcc ctggcgctcc atctccagcg caaagtcaat catcatgatg gcattcttct
     5701 tcacgattcc aatgagcaac agaatgccaa ccagggcaat cacgttcagt tccatgtgaa
     5761 agagcatcag agctagcaga gcgccggtac cggcggacgg gatggtggaa agaatcgtga
     5821 tgggatggat atagctctcg tacaacatcc cgagaacgat gtacaccgca agcagcgcag
     5881 ccagaatcag caggggctcc gatgaaagcg aatcctgaaa ggcggcagcc gttccctgga
     5941 agctgccatg gatggtggcc ggaaagcgca tctgcaactg cgtctgctca atcagtttgg
     6001 tagcatcgcc caaggccgcg ccgggcgcga ggttgaacga gatcgttact gccgggaact
     6061 gtccctggtg ggcgaccgcc agggatgtac tggtcggccc gaaatgcgcg aacgcggaaa
     6121 gcggtacctg cgcccctgtc gaagccggag tggctgtcgt agcgggtgtg actgttgcgg
     6181 ccgatccacc gtttgcaggc tgcccactgc tcaaggtcgt cgagccactt accgtcgccg
     6241 ggatacccgt cggcacctgc gctccgggtc tggtaatcat ataaacgctc tgcagcgcat
     6301 ccggagtcat ttgatattgc gggtcaacct cgagcacgac gtgatactgg ttcagcgtct
     6361 ggtagatggt cgagatctgg cgttgtccga aggcgtcgta aagcacgttg tcaatctgcg
     6421 agggcgtaat acccagtcgc gcagcggtat cgcgatcgat gatgacattt tgctgcaagc
     6481 cgcgtacctg ctggtcgctg ctcacatctt tgaggatggg aatctgcttc agcttgtctg
     6541 tcagtttagg cgcccacgcg tacaactcgg ccaggtcctc gcccgagagc gtgtattggt
     6601 attgggcatt ggcaaagcgg ccaccaatct gcaaatcctg cgcggcctgg aaaaacaggg
     6661 tcgctccggg cacgtgggaa gtcttcgggc gaatgcggtt gatcacctgg tcgatgctga
     6721 ccttgcgctg gttgagcggc ttcaaggtca cgaacatgcg accctggttc tgcgccgtgt
     6781 tgccacctga aaacgcaatg acattgtcaa ccgcgggatc gcttttcacg atcttgatga
     6841 actcggccac ccgctcccgc attccagcga acgccatgtc ctggtcagcc atgatggtgc
     6901 ccatcatgcg accggtgtcc tgttgcggga agaagccttt gggcacgatg atgtacaggt
     6961 acacgtttac aaagacggcc aataccgcaa ttgcaaagac cagccgctga tgacgcaaca
     7021 cccatgccag ggaccgttta taccccgaat taaggcggat aaaacctttc tcgctggccc
     7081 ggtaaatgaa gccatgttta gcgccgcgag gtttcagcaa tcgcgcgcac agcatcggcg
     7141 tcgtggtcaa cgacaccacc atggaaaccg caatggcccc gctgatggtc atggcgaatt
     7201 cgtgaaagag tcggccaatc aagccgctca tcagcagaat cgggatgaac accgcgatca
     7261 gcgaaatgct catcgacacc acggtaaagc cgatctcacg ggcccccagc agcgcggctt
     7321 cgtgcggggc catgccggct tcgacgtggc gcatcacgtt ttccaggacc acgatggcgt
     7381 cgtccaccac gaacccagtg gaaatcgtca acgccatcag ggacaaattg ttgagcgaat
     7441 agttcaacag atacatgatc ccgaaggtgc cgatgatggc gaggggcacg gcaacactgg
     7501 gaatgaaggt agcgcggact tcgcgcagga aaacgaacac caccagcaca acgagaatca
     7561 gcgagatgat cagcgtccgt tcaacgtcgg ccaccgaagc ccgaacggtg gtggtgcggt
     7621 caagcgcaat gtcgagcttg atgttctgcg gcatcgatgc cttcaagaaa ggcaactggc
     7681 cgataatccg gtcaaccgtg gcgatgatgt tggctccggg ctgccggaaa ataatgatca
     7741 tcacgcaggg tttgccattg aagcggccgg cgacgtgggt gtcggtgtag gaatcctcaa
     7801 catcggcgac gtcgctgagg cgcaccggag ccccattcct gtaggccacg atgatcggcc
     7861 gataatcctt ggccttcttc agctggtcgt tgtcgctgat catccagctc ttgtcgccac
     7921 cctgcagcga gcccttcgcc tggttcacgt tttgcgccgc aaccgcggtg cgaacggcgt
     7981 ccaggccaat gttcaatttg ttgagcaggt tgggattcgc ttcgatgcgg acagcgggct
     8041 gggacgatcc accaacgtac acctgcccca cgcctgcgac ttgcgccaac ttctgggaga
     8101 ggatcgagtc ggccgcatcg tacatctggt cctgcgtcat ggtgtctgac gtcagcgcca
     8161 gaatcaggat gggggaatcg gcgggattca ctttgcgata gctgggattg cccggcaaat
     8221 ccgcaggcag gtatccacgg gccgcgttga tggccgcttg cacatcgcgc gctgccgcat
     8281 caatattgcg gttcaaatcg aactgcatgg tgatgcccgt cgatcccaac tgactggtcg
     8341 aggtcatctg attgatgccg gcgatgcggc cgaactgccg ctccaaaggc gtagccacgg
     8401 aagtcgccat ggtctccgga ctcgcgcccg gcagccccgc gccgactgag atcaccggga
     8461 aatcaatttg cggcaggggt gagacgggaa gctgcgtata agccaagcct ccagccaaaa
     8521 gtagagcgat ggtgagaaga aaagtcgcga ctggccgctc gatggctggt ccggagacat
     8581 tcatgcttaa tctcccatgg cctctgcggg cttcggcgca cggatattgc gcagcgcggc
     8641 gcccagacgg tcaaaccaca ggtaaaccac cggagtggtg tagagcgtca agatctggct
     8701 gaacaacaga ccaccgatga tggtcacacc caaggggcgt cgcaactcgg aaccggtacc
     8761 cgaacccaac gccagtggca ccgcgccgaa caatgctgcg aacgtggtca tcatgatagg
     8821 acgaaagcgc agcaggcagg cctcgtagat ggcttccagc ggcgccttgc cttccttgcg
     8881 ttcagcttcg agcgcgaagt cgatcatcat gatggcgttc ttcttcacga taccgatcaa
     8941 caatatgatg cctatcaacg ccatcactcc caggtccatg cggaagatgt tgagggcaag
     9001 aatcgcgccc acgccggctg aaggcagcgt cgacagaatg gtgactggat gaatgtagct
     9061 ctcgtacagc actcccaaga cgatgtacac ggtgatgatc gctgccagaa tcagccagcc
     9121 ctcgttggcc tgagacgcct cgaaagccct tgccgttccc tggaacttca cctggacgct
     9181 gggcggcaac ccgacctccg ccaccgcgcc attgatcgcc tgcaccgctt cgcccagcga
     9241 atatctgccg cccaggttga aggagatggt caccgccgga aactgccctt gatggttcaa
     9301 cgacaatgcg ccggtgccgg tctcgaaatg cgcgatggca ctcagcggga cctgcccggt
     9361 ggtgccgggc acgtagatgc tcttcagcga atcaggatcg tcgcgaaacg acggatcaac
     9421 ctctagcacc acgtggtact ggttcaactg ggtgaacatg atcgagatca ggcgctgccc
     9481 aaaggcgtcg tagagtgtat tgtccactgc gctggcgctc acacccaggc gcgaagcggt
     9541 gtcgcgatca atctggagat tggcgaccgc tgcctggttc tgctggtcgg ttgcgacatc
     9601 gcgcaactga ggaagtgtct tcaacttggc catcagagtg ctggtcgact gcgccagttc
     9661 tttgggatca gcatcctcca ggctgaactg atactgggtg cggctcaccc ggtcctcgac
     9721 cgacaggtct tgcaccggtt gcatgtagag cgtgatgccc gcgactccgg ccgtctcctg
     9781 ttgcaaacgc ttgatgatgg cggaaacatc gtcgttgcgt tcgtccttgg gcttcaggtt
     9841 gatctggaaa cgcccgctgt ttggagtggt atttgtcccg tctacgccga tgaatgacga
     9901 caagctggct acatccgggt ccttcagaat ggccgcagcc agcctgactt gccgttccgc
     9961 catggacgcg aacgaaacgc tctccggcgc atcggaaatg cccatgatca cgcctgtgtc
    10021 ctgaatggga aagaagccct tgggcaccag gatgtacagc cagatggtcg ccaccagggt
    10081 gcctaacgta atgatcaatg tcagcgtttg gtgttccagc acaaccttca gggtgcgccc
    10141 gtagaatgcg attgtgctgt tgaaaacccg ctccgacgcc tcgtacaact tgccgtgctt
    10201 ttcctggccc ttctggcgca acaggattga gcacatcatg ggcgtaagcg tcagcgagat
    10261 aaaggccgac aacagaatgg tcacgctcaa ggtcactgcg aattcgcgga atagccggcc
    10321 cacgatgtcg cccatgaaca gcagcgggat cagcaccgcg atcagcgaaa ccgtcaacga
    10381 aacaatggtg aaaccaatct gtccggagcc gcgcagtgcg gcttccaagg gcgagaagcc
    10441 gtcctcgata tagcggtcga tgttctcgat catgacgatc gcgtcatcga ccacgaagcc
    10501 ggtcgagatg gtcaacgcca tcagcgacag gttgtcgagc gagtagccca gcaggtacat
    10561 cacgccgaac gtgcccacca gcgacagcgg cacggcgatg ctgggaataa tggtcgccgc
    10621 caacgtgcgc aggaacagga agatcaccaa cactaccagc gcgatggtca gcatcaggct
    10681 gaactttacg tcggccacgc tagcgcggat ggtgttggtg cggtcggtca gaacggttat
    10741 tttgacggaa gccggcaggt tggcctgaag ttgtttcaac cgcagcttca cgctgtcggc
    10801 aaccttgata atgttggccc cgggctggcg ctgaatgttc agaatcaccg ctggagtggt
    10861 gttcatccac gccgcctggt tcaggttctc cgggccctga atcacagtgg ccacatcgga
    10921 cactcgaacc gggttgccat tcttataggc aatgatggtc ttggcgtaat cctcgggttt
    10981 gaacaactga tcgttggcgc caatcgtgta agcctggcga ttatcctgca gttgcccctt
    11041 ggcctggtcc acattgaccg cggcgatcat ggtgcgcaat tgctccaggc tcatgccgta
    11101 agacgccagc gcggtcgggt tcgcctgaat tcgcactgcc ggcttctgcc cgccactgat
    11161 ggaaaccagg cccactcccg gcaattggga aatcttctgc gcaaacgtgg tgtccgccag
    11221 gtcctcgatc tggggcagag gcatggtgtc ggaggtcatg gccagcgtca aaatgggcgc
    11281 atccgcaggg ttgaccttgc tgtagatcgg aggcgtcggc aggtcgggcg gcagaaacgt
    11341 ctgcgcactg ttgatggccg cctgcacttc ctgttcggct acatcgatgt tttcatcgag
    11401 attgaattgc agcgtgatga ccgacgcgcc aaacgaactg ctcgacgtca tctggtttag
    11461 tccgggtagc tgtccaaact gtctttccag cggcgcggtg atcgacgaag ccgtgacgtc
    11521 gggactgcct cccggataaa tcgtcacgat ctgaatggtt gggtaatcca cctgcggcag
    11581 ggccgaaatc ggcagttgct ggtaggccac gatccccact agcagcagag ccaccataag
    11641 cagcgaggtc gcgatgggcc gcagaatgaa tggccgcgaa atactcatga gtgcaatccg
    11701 ttaccctcgg ctggctgcgg actcttggat tggttgaccg gcgcctgcac actgacgcgg
    11761 cttcccggct gcagcttctc ctgtccgtcg gtgacaacct tgtcgccagc ctgcaagccc
    11821 gactcgatca cgacgacgtt gccctgtgtc aaggcgatct gcaccgcttt cacctgtacc
    11881 gtgttgttgg cgtcaaccgc ataggtaaag gttccctgag gcccgcgttg tattgcgctg
    11941 gcgggcatgg taagcgcatc cttgcgcgtc tccagcaaca ggtggacgtt cacaaactga
    12001 ttgggccaga gattgttgtc ggggttctca aacaccgcct taaacttcgc ggtcccggtg
    12061 gtttggtcga tctgattgtc gatggtcagc agcttgccgg tggagagctt ggtctggtca
    12121 tcgcggctat atacatccac caccagcgtt ccttctttca tacgctgccg caccgcctgg
    12181 agctggtctt ccggcaaggt gaagtcgatc gcgataggat gcagctgcgt aatgatgacc
    12241 attggggtgc tggatgcggc ttgcacgtag ttgcctatat ccacctgccg caggccgatg
    12301 cgtccgttga tgggcgacgt tatgcgggta taaaccaggt ttagattcgc gctgttaatc
    12361 gcagccttgt cagcagcgat ggtgccttcg tactgcccga acatagcctc ctgcgtgtcc
    12421 aggtcctgct tggcgatcac gccgccggca tatagcgctt gcatgcgcgc gtactgcacc
    12481 ttggcgttat tcagttgggc ttcatcgcgt tgcagattgg ctttcgcggt ttgcagtgcc
    12541 acgtcgtagg gacgcggatc gatctggatc agcaactgcc cgaccgatac atcctggcct
    12601 tcctggaaat tgacctgcat gatctggccg tcaatgcggc tcttcagcga taccgtgtta
    12661 aaagcctgca ccgcgcccag cccggtgagg tagacaggaa agtcgtgttg ctggacggtg
    12721 gcgacaccga cgggtaccga cgggggtggc ccgctcgcct tggcattctt gtcggcgcgg
    12781 ctgcaacctg cgagaacggc gcacagcaga agggcgattc ctactttatg aattcgaagc
    12841 aacgtcaaac cttcctcctc gatgacgggc ggctttgtgg cagggttgga ggccgtcgcc
    12901 agggggcacg gtacttgatc aagaccatgc cagttacagt agtattaatc ccacctaatt
    12961 agaccggata gccagtccga gggccgttaa gagagggtga aggaaggtta agttttgtga
    13021 agggtccgaa cacatcccga aacaattctt caccgcagtc acgcactgga aaaggcacaa
    13081 tgaaaccagt gaaccatctg ctcatcatcg acgacgacca tgctctggtc aatctgctga
    13141 agcgctttct ggaggcagag ggctttcaca tcgacgctgc ctacgaccat gataccggac
    13201 tggctgccgc cctcggcgat caccacgaat tgattattct cgatgtcatg ctgcccggcg
    13261 gctccggatt cgagttgctc aaggcgttgc gtcatcaatc tgccgttccg gttctgctgc
    13321 ttaccgcccg cggcgaagcc gtcgatcgca tcctgggact cgagatcggc gctgatgatt
    13381 atctcgcaaa acccttcgat ccgcgtgaac tggtggcccg catccgcgcc gtactgcgac
    13441 gcactcgcga agcggcttcc ggcacgcccc gcggcgatca tgacgaggtg ctgaacgtcg
    13501 gcgatataga actctcgctc ggcacccgaa acgtcacctg cggcggtaag cccatcgaac
    13561 tgacttcggt tgaattcaac gtactggagc tgttgctgcg caatgccggc aacgtggtca
    13621 ctcgcgaaca gatcgccgaa gttgcgctgg gacgcccgtt gaactccttc gaccgcagcg
    13681 tggacgtcca tgtaagccgg ctgcgcaaga aactcggcag ccttcccggc aacgacgatc
    13741 tcatccgccc catccgcggc attggctatt tcctcgcctc cgccacgccg gaaaagagca
    13801 aagcatgaac cggcggctgt tctggaagat ttttctcccc ttctgggttg cgcaagcgtt
    13861 attgctcggc atgttgtacc tgcgcctgca ttaccggctg catgccgaga acccctggtg
    13921 ggtgcagccg gaacggcgcc tcgttcccac tctggcggac ctcgcagtcc ggcgctatga
    13981 ggagggaggc cagcctgcgc ttcgcgcttt gctcgatcag acggccatgc cgcaccgttc
    14041 cgactattgg ctgctcgatg ctgctggccg cgagttaagc ggacgccccc tgccggacga
    14101 gattatcgac tttgcccaac gtgccttgcg gcacgagggc atggtacgtc ccgacgaggc
    14161 cgtggtcctg gtcgctcccg tcgtcacccc tcgcagcagc tacttgttga tcggcgaatt
    14221 cattccaccg ccgctctcgg aacgcgttcc tggcgacatt ctatgggtat taaaactcgg
    14281 caccatcatt tctgccctga tgtgcctggt catcgcccac tatctcagca agcccattga
    14341 acggctacgc ctcgcgacca acgaactggc gcgcggtaat ctcgacatcc gtgtgggcgc
    14401 caccatcggc aagcgtagcg acgaaatcgc cgacctggta cgtgacttcg attccatggc
    14461 cggagagttg cgcaaccaaa tccaaagtga acgcaatctt ctgagcggtg tgtcgcacga
    14521 actgcgttcg cccattgccc gcatgcgtct ggccctggct ctggcgcgca cctccgaccg
    14581 gcccgagcgc gatgagatgc tcgaccgcat cgagcaggac accattcagc ttgactcgat
    14641 gctggagcgc atcctgaccg tcgcccgttt ggagaatgga caatacaagc cgaaattcga
    14701 gagcctctcg ctcaacgatc tgatcgatga agtgctcgac gacgcgcgct tcgaggcggc
    14761 cgcaactgat gtcaccgtca actacgctga cgacgccatg ctgcacgtca acggcgatcc
    14821 agggctgctc cgaagtgcca tcgagaacgt agtgcgcaat gccatcttct actcggggca
    14881 aggcggcagg atagatgtcg gtctgcgtct cgacgacagc cacgccattc tgaccgtccg
    14941 tgacaatggt aagggagtgc cggacgatgc tctcgccctc ctcttcaagc ctttctatcg
    15001 tgtggacgac gcacgtggaa ccaccactgg cggcatgggc cttggactgg cgatcgtacg
    15061 caatgccgta gctgttcacg gcggaaccgt tacagcccga aacgtctcgc cccacggttt
    15121 ggaagtggaa ttgcggcttc cggttgcggg cagcgcgctt cccgccggta actcggcggc
    15181 gcaaatggtc gcgcccctgc aaggtcggcg ctgacttttc ccgtcgttac cccatactgt
    15241 gggtgcccca tcctatgcgc cccgcatttg ggcgcatagg gtgggaatac aaactctccc
    15301 acattgtcag acctgcaata accctgaata aatcgccatt cccactacag catccacgcc
    15361 catcgtatcg agcgtgtcga tctcttgctg gctgcgaata ccccccgccg cgatcagccg
    15421 ccgcgacgtc agcctgcgca acccgtccac gacgctcagc ggaatcccct gcaataatcc
    15481 ctcggtgtcg atatgcgtgt agaggaacgc cgaacaagag ggctccagcg cctgcatcat
    15541 ctccgccgga gtaatcgcgg tggtggtctt ccatccgtga atggccaccc gcccaccgcg
    15601 cgagtcgatt gccatcacca gtcgttcggc gcctaactcc tcgcacagcg tgcgggcaaa
    15661 cacagtatcg acgccactat ctttaatcaa tgacgacccc agaatgaccc gcttcgcgcc
    15721 cgccgccagc accttccgcg cattctcgcc ggagcgaatc cccccgccta cctggcacgg
    15781 cagtttgctg cagatatcgg cgatcagcgc ctggttgctg ccattgccca tcgccgcgtc
    15841 gaggtcaatc aattgcacca gaggataacg cgagaatcgc tcgatccact cgccgaagtt
    15901 gtcgaactcc agtgctttct tctccccttg cactagctgg acgatcctgc cgcccatcag
    15961 atcgatcgag ggaatcagca cgtgggcctc accgggatgc ccgcatcccg cagttcctgc
    16021 ttgatcgccg ccaccgactt caggccgaaa tggaacaccg aagctgccag agccgcatcc
    16081 gcggctcccg cctgaaatac ctccacaaag tggtgaggtt cgcccgctcc gcccgacgcg
    16141 attaccggaa tacgcacgct ggtcgcaatc gtccgggtca gcgggcagtc gaaccctgac
    16201 ttcgtgccat cacggtccat cgaagtcagc atgatctctc ccgcgcccaa atcctccgcc
    16261 gcgatcgccc actgtttcgc atcccgcccg gtcggcttcc ggcctcccca gacgaatacc
    16321 tcccacgctg tgggagttcg ctgccgggcg tcaattgcaa ccaccacggc ctgcgatcca
    16381 tagcgctcag ccacctgcgt aatcagtgtg ggatgggcga gggccgccga gttcatcgcc
    16441 actttatcag cgccgcagtc tagtaccgcc gctgcatcgt cgagcgaccg gattccaccg
    16501 cccacggtga aggggatgaa cagctgcttg gccgtgcgcc gcaccgtatc cagcagcgtg
    16561 ttgcgcgatt cagccgtggc cgtgatgtcg agcagcacga tctcgtcagc ccccgcttga
    16621 ctatgcgcca acgccagttc agccggatcg cccgcatcgc gtatttgctc gaattggatg
    16681 cccttcacca ctcgccctgc attcacgtcg aggcaggcaa tgatgcgttt ggccagcgtt
    16741 tcacaatgca cagaagttct ccaggatatg caggcccaca tcgcccgact tctcgggatg
    16801 gaattgcaca ccgaacagat ttccccgctc caccgccgct gtaaacggct cttcgtaggt
    16861 acaggtcgcc accgtgtctt cgcacactgg cgcgcgatac gaatgggtga aatacacata
    16921 cgcgccggaa gcaattcccg ccagcagccg cgaacgcgaa tcgatggcaa tgctgtccca
    16981 accgacgtgc ggcgacttca ccagatcgga gaaccgtgtg cactcccccg caaacgtcgc
    17041 cagtcctcgc aattccgggg cctcgctgct gccttcaaac atccattgca acccgaggca
    17101 gattcccagt aatggccgtc cctcgggact ggccgcctcc cgcaatacct ggccaagttt
    17161 tccgttgacc agcggcgccg ttgccttgaa attcccgact cctggtatga tccatttatc
    17221 tgcacgtccc acaatgtcag ggtgattggt cacggcacat tcttttccca ggtgctgtaa
    17281 ggcctttacc acagaggtca agttccccgc gccgtaatcc acaatcgcaa tcacaacaac
    17341 cccttcgtgc tggggagcat gcgtcccagt tgcttgtctt tcgaacacgc cacccgcagc
    17401 gccctcgcaa aggctttgaa caacgcttcg atcttgtgat gattcgaacg gccataaaga
    17461 gtcttgacgt ggacgttggc cttcgcgccg cgcgagaacc cgtcgaagaa gtcagccact
    17521 aactcggcct gcagatcgcc caccagtcga ctgcgcacct tggtatcgac gaccgccgcg
    17581 ctgcgcccac cgaaatcgac ggcggccaag cctagcgtct cgtccatcgg catcacgaaa
    17641 tatccggcac gaagaatgcc tcgcttgctg cccagcgccg cggtaaatgc ctctcccagc
    17701 gcgatgccca cgtcttccac cgtgtgatgt tgatcaacgt cgagatcgcc ggcggcctgc
    17761 agctcgaggt caaacgcgcc gtgccgcacc accaactcca tcatgtgatc gagaaaacgt
    17821 atgccggtgc tgaccttcgc tcgcccgtgc ccttcgatgg tcagtgcgat gcggatatcg
    17881 gtctcgttag tcttgcgatg gatgcgcgcc cgcctcatgc cgataccccc gctcccagtt
    17941 tcaactcggg caccacgtcg cgcaacgcga ccagcagccg ctcggtttgc tcgctggttc
    18001 ccagggtgat gcgcacgcag ccgtcacacc ccggatcgga cgagcggtcg cgcaccaata
    18061 tcccacgttg tcgcatagcg gccacgaagt cgcaatgccg ctttccgatt tgcatcagca
    18121 cgaaattcgc ctcgctcggc caccacgcga tgcccgctcg tgacaggtcg ttctcaagtt
    18181 gcgcgcggcc ggctttcacc tgcaaaacgt aagccgcaac gaacttactg tctgccagcg
    18241 ccaccggcag cgccagcagc gcgaccgcat tcacgctata cggcgacgac actcgccgca
    18301 ccatcggcat ctgctcccgc ggtcctgcga gaatgccgat gcgcaggccc gccagcccat
    18361 acgccttgga aaacgtccgc gccacaaaca gattgggctg tgttccaacg tcggcgatta
    18421 ccgtcttccc gtagaaatcg tagtacgctt catcgacaag taacgccgcg ccaggcgcat
    18481 tctttgcgat ggccagcagt tgctcacgtg atgccactcc gcccgtggga ttattgggac
    18541 tggccaccgc aatcagcttc gtcgccccac taatcttggc cagcaccttt tccactggaa
    18601 agcgcaggtc ctcgtcacac tgcacccgga cgaccgtcgc tcccgtcgct tcagcgaaga
    18661 tttcatacat gctgaacgtc ggagttacta cgatgacttc gtccgacggt tcgagatacg
    18721 cttcgcacaa gaggtgaatg ccctcatcca cgccgttggt gagcagcacc tgttcggccg
    18781 gcagtccgag atacgcggcg gcgatcgcct ctaccggctc gcgctcggga taacgcgcca
    18841 aatcctcggc ggtgatctcg cgaatgcgcg ccagcacgcg cggcgagcat ccctcggtgt
    18901 tttcgttgaa gtcgaggcgc agccctttcc gtcccgccag aggaggatga tactcgtgca
    18961 tctgttgcac agctttgcgc gcgctaagca ttgccggacc tcactcgcac cgactcggca
    19021 tgggcgcgca ggccctcggt ttcggccagc ttcgtcacta ccgacgcgat ctcgcgcagt
    19081 ccctcgctcg tgaacttctg caccgtgatg atcttcacga agtcggccac gcttaatccg
    19141 ccgcgaaaac gcgccgcacc ggcagtgggc agcacgtggt tcggtcccgc tgcgtagtct
    19201 cctgccgact gcggcgagta gttgccaaca aacaccgacc ctgcgttgcg aaccgccatc
    19261 aagtccgagt cgtcgtctac agtcagatgc tcgggcgcga tgcggttcac ccaatcaatc
    19321 gcctgctcgc gcgtcgacgc aaccagcgca taggcgttcc gccgcaccga ctgctgcgcg
    19381 attgcattgc cttttgcctg gcgctccacg ttttgcgtca catcctccgc aaggtccttc
    19441 ttggtggaga cgaacacgac aactgcgtcc gggtcgtgct ccgcctgcga caccaaatcg
    19501 gacgcgataa attccgtgtc gccattgtcg ctcagcacca ccgcctcggt tggtcccgct
    19561 agcatatcga tcgcgcactc gaagctcact agcttcttgg ccgcagtgac gaacgcattg
    19621 cccggcccaa caatcttgtc cactcgcgca atgctctcgg tcccgtacgc caacgccgcg
    19681 atcccttgtg ccccgccgca acgataaacc tcggtaatcc acaataaacg cgcggctgcc
    19741 agcgttgctg gttgcggatt cggtgtgacc accacgatgc gttctacccc cgccacttgc
    19801 gccggaatcg ccgtcatcag cagcgtcgac ggcagcggat atcgccctcc cggcacgtag
    19861 catcccaccg acgccagcgg tcgcaccacc tgccccaact ccccgccgct gatattcttg
    19921 cgccacgact tcggcatctg ccagttggca aaccgccgga tattggcagc cgcgcgcttc
    19981 aacgcatccc gagtgtccgc cggagtcgtc tcccacgcct gctccatctc ctgttgtggc
    20041 actcgaatcg gttttccctt ttccagcttg tcccacaatg tggcataccg aaccagcgcc
    20101 ttgtcgccgc ctttgcgcac gccctgaata attttgcgca cgttgggctc aacctcttcc
    20161 agccgcgacg cccggccttc caatttcgcc accgcgcgtt ccgcagcttt cccctcgagg
    20221 attcgcatca catcacgacc ttatttagcg gatactccac gatgcctgtc gcattcgccg
    20281 ctttcagccg cggaatcacc tcccagcaca ggctctcttc catgatggtg ttcaccgcga
    20341 cccacgcctt gtcgctcagg gtggaaatag tcggactgtt cagcgccgga agcacggcca
    20401 gcaccgcctg caggtcttcg accctgacgt tgagcataag gcccacgcgt ccctgagctt
    20461 ccatggcacc gcgcagcatg agcgcgaggt tttcgatctt ctgccgctta ccggcgtcgg
    20521 tccacgcttt tttgttggcg atgatttggg tgttggattc gagcagggtt tcgatgatgc
    20581 gcaggcggtt ggccttgagc gagctgccgg tttcggtgac ttcgacgatg gcgtccgcca
    20641 atgtgggagg cttaacttcc gtcgcccccc aggagaactc gacttttacg tctatcccac
    20701 gctgtaggaa gtagcgagtc gtgacgttga ccagctcggt cgcgatagtg gcgccccgca
    20761 ggtcttccgc acgctgcgcg ggcgagtctt ccggcaccgc cagcacccat cgcactttcc
    20821 cccggctttg cttggcgtag atcaagtcag ccacggtgac gacttcgagg ccgctttccg
    20881 taacccaatc gagcccggtc attccggcat ccagcgcgcc gtgctcgacg tagcgcgtca
    20941 tctcctgcgc tcggatcagc atgcactcga tttcaggatc gttggtgccc gcaaagtacg
    21001 atcgcggact gacatacacc tgcagtccag cgcgtgccag cagcgccagc gtcgcttcct
    21061 gcaggctgcc tttgggaagt cccaatcgca gcttcatcgc ccacctcccg tcgcggtcgc
    21121 caccaacagt tcggtattga agcaggtgcg cgtgccggtg tgacagacta cgccctgccc
    21181 ttctacttcc acgcgacaca ggacggtgtc gaggtcgcaa tccgccagcg cgctgatcac
    21241 tttcaggcga ttgcccgagg tctcgccctt ggtccacagc tcgttgcgag ttcggctgta
    21301 gaacgtgaca tagcccgtgg ccagcgtctt atcgtaggcg gcgcgattca tgaagcccaa
    21361 catcagcact tcgcctgtgg ccgcgtcctg cacgatggcg ggtgccagcc ccatcatctt
    21421 gtcgaaatct atttcaaccg gcattccctc ttcctttcgc caaaacaaaa aacccgctga
    21481 gcttatgtca gcgggcagga ggtataagta cgaaaagcct atacgatgat ctccgctgac
    21541 acgttcgcca cgtgcgaatg gtgatgatga tggcgatgga ccaacgaagt cattctagtt
    21601 tgaaattacc ggatcgccgg acctctgtca atgtaacttt tcggaccggc ccgccgaaac
    21661 ccgtggcttc agcatgtcac tgcgaatgcc gcgaacgtgt gctactttta tccctttcag
    21721 aaaggcaccc tcatgtttac cagcgatctt ctaaagaaca agcgttttct cgtcaccggc
    21781 ggcggcacgg gcatcggccg cgctcttacc gagcgctttc tacaactggg cgcaaccggc
    21841 tacgtctgcg gccgccgcgc cgaagtgatt caacagaccg caaccgaact ggcggcttcc
    21901 actggaagcg ccatcgagtc cttcgtttgc gacgtgcgct atagcgacga cgtcgacaag
    21961 gtcatcgagc agatctgggc caaaggtccg ctcgatattc tcgtcaacaa cgccgctggt
    22021 aactttctgg caaaaaccga aaccctttcg tcacacgctt tcgaagccgt aatcgggatc
    22081 gtgctcaacg gcaccatcaa catgactctc gcttgtggca agcgctggct cgccgccaag
    22141 catcccggca tggtgctcaa catcgcgacc acctacgccg acaccggctc cgcctatgtg
    22201 gttcccagcg ccgtcgccaa agccggagtc gtggccctca cccgcagtct tgcagtcgaa
    22261 tggggaggga gaggcgtgcg cttcgcggcc atcgcgcccg gagctattcg caccgaaggc
    22321 gcattctcgc ggctgctgcc tgtgcccgaa atggagaagg cgctgctcga ccacaaccct
    22381 ctgcatcgcc tcggcactct cgaagaaatc tccaaccttg ccgcgtttct ggtgagcgac
    22441 caagcgggtt acatcaacgg cgaaatggtt tacatggacg gaggcgaaat gctggccggc
    22501 gccagcgtgt tcagcctctt gggacgcaaa gtccccgacg tcgcctggga gatgttgaag
    22561 ccgaggaaga agtaatccgc aatacgaggc cgcgatgacg tcgcggcctc cagttaagtc
    22621 ctcagcagtc tcgaactaga acctgtatcc cactccaaac tgaatcaccg gaaagacctt
    22681 gtacggcgac gcatcgctgt tgtacttcgc ctcctgcgct ttgatgttcg cttgcacggt
    22741 cggatcggaa ctgatcgccc ggcagtttgt ctgaccgaag tcacacacgt cgccggtgag
    22801 attcatagtg acattcggcg atccccaaaa cagcacgccg gcatcaaaga tcacgctcca
    22861 atgtcgcgag cgcggcaaca gggtgcccca tccaaacaga atctcggggg ccaccttgtt
    22921 aagcccgatg gtggcgcttc cagttacggg ggtggtcgta ctgctgaagt acggcgcgcc
    22981 atttaaggtg aagaactgcc ctcctgtaac actggcgctg cccgtgattt tgttgccgtt
    23041 gtacaccaga agcccagggc tcaggtggaa accgttggcc cacgggaacc aatccaacag
    23101 caccccaaca ttgccgaggt gaatagaggc atcgtaggta attccattgc ggacggaact
    23161 gcggctgatg cggaaaaagt tcagttcccc gcggacgtcc aatttccggt taagaccgac
    23221 cgctccctgt cctcccaagc ccagcgtgcc gaagacaaac cctatgccga ccttatgcgc
    23281 gtcctggtcc gcgctctgag gtttgggggc tggttggcca ggggtctggc tttgactact
    23341 gttggtggca tctggtgcag gaaccgagag ctcggtcgaa ctgtccgtgg ttcccattgg
    23401 gttggggtct gacgccgtcg ctgtcatcga caaagtggcg accatcacga tgagcgcaag
    23461 cgcagggtag aggactcgag ctttcattag ctcctccgcc gtcgaaattg gccgaggaat
    23521 tagagcaccc tcggtcacac tattttgcac tcgaatgttg aggtgtcaag cgcattcgtt
    23581 aaacgcgttg gtggtgaatc aaacttctta ccaaacccct cacaaaagag gcgttctcct
    23641 gccctaattt cgtgcaaaag acgggcgacc cgcaggccgc ccgaccagtt accagctacc
    23701 agtcaccagt tacctaattc aacacatgaa tattcttctt ctcgcacatc tccttcagca
    23761 cctttggcca caccgtgacg ctcacttcgc ccaggtgcgc cttgcgcaga atcagcatca
    23821 tggtgcgtga ctgcccgatc cctccgccaa tgctcagcgg gatctcgtga ttcacgatcg
    23881 cctggtgata cggatacttc aggaagtcca tctggtgggt aagttccagc tgcttcttga
    23941 gcgactccgc attcacccga atgcccatcg acgtcagctc atgccgacga tgcgtcaccg
    24001 ggttccacac caggatgtcg ccattcaggc cgtgcatcat gtggccattc ttggtgatcg
    24061 taggcgtgac ccagtcgtca tagtcggccg cccgcatctc gtgcggatag ccgtcgcgta
    24121 gcatccatcc aatgccgatg atgaacaccg ccggatactt ctgcaggatc gccgtctccc
    24181 gctgtttgcg cggcaggtcg ggatacatgt cgagaatttc ctcggcatgc aggaatgtga
    24241 tctgctccgg caggttggga tacttcggat cgcacaacgc ctggaacaac tcctgcgcgt
    24301 agcgctcggc cccgacaatc accttccaga tcttggtcac gatgcctttc aggaagtcga
    24361 gattgcgctg ctcgggagtg atcacgcgct cccagtccca ttggtcgacg taggcgctgt
    24421 gatcgtgatc gaggaagtaa tctttgcgca cggcgcgcat gtcggtgcac aggccttcgc
    24481 ccgccgccat gccgaactgc ttgagcgcca ttctcttcca cttggtggca gcctgcacca
    24541 cctgcgcatt gatgggatgc ttgtcgcgat cgttggagat gtggaacccg atgggtgttc
    24601 tggatccgtc gcggtccagc atgtcattca cgccgctctc ttcgtccacg atcagcggca
    24661 cttccaccat catcaggttg agttccttgc agaggttgtc ttcgatgtac cgcttcacct
    24721 cgaagagcgc tctctgcgtc tctttcggtg tcagcagcga gctgtagttg tccggcagga
    24781 ctttttccag ttccaggtaa tcgccaatgc cgggtccagc gaggtccgct ttcttgtcag
    24841 ccatgctgtg ctccttatga ttgcggtcgg cgcgcggcaa gtcctgctca gcgggcttag
    24901 tcgcggcagt ccacacgccg ccatcctaat gaaagcactg gccgaacgtg cccgctgtaa
    24961 cctgcgtcac gtggctttgt gccgtgcggg cgcggagaac cttccacccg tcgcccggaa
    25021 tgttactctt gtatcgtttc gctggcgcac tggctcttgc tgcgcccgcc gcgagtggcg
    25081 ctctgtcacg cccgccgcga ggagaacgcg accatggctc catcggatcc caaaaagcaa
    25141 gttccggcca aggaagaagc tcccgccatc gagcccgccc ctgccccaga gcaaggcgaa
    25201 gaccagggct cggtcctgct taccagctat gacctctacc tgttcaacga gggtaagcac
    25261 aaccggctgt atcacaagct gggagcccat ctcaccgaaa tcgatggcta tcccggaact
    25321 tatttcgccg tctgggcgcc cgacgccgag cgtctctccg tcgtcggaga cttcaacggc
    25381 tggaactccg actccaatcc catgacgccg cgtggcgact cgggcgtgtg ggagtgcttc
    25441 gtccgcggac tcggcaaggg atcgatttac aagtaccact tgcgctcgcg cttccatatg
    25501 taccaggtca acaaggccga cccctacggc tttcatcacg agacgccgcc gcgcactggc
    25561 tccatcgtct gggacctcga ctaccagtgg ggcgacgaag actggatgag cctgcgccac
    25621 aagcgcaacg ctttcgacgc gcccatgtcg gtgtacgaag tgcacatcgg ctcctggatg
    25681 cgcaatcagg aacagggcaa ccgctggttc agctatcgtg aactcgctcc ccggctcgct
    25741 gcctacgtca acgagctcgg cttcactcac gtcgagttca tgcccgtgat ggagcacccg
    25801 tacttcgcct cctggggcta ccaagtcacg ggatacttcg cgcccagcag ccgcttcggc
    25861 acgccgcagg acttcatgta cctggtcgac tacctccacc agcggggcat tggcgtcatt
    25921 ctcgactggg tgccctcgca cttccccaac gaccagcacg gactcgccta cttcgacggc
    25981 acgcacctct acgagcacgc cgatccgcgc aagggatttc atcccgactg gaagtcggcc
    26041 atcttcaatt atggccgcaa tgaagttcgc ggcttcctca tcagcaacgc catgttctgg
    26101 ctcgacgagt accacattga cggccttcgc atggacgcca tcgcctccat gctctacctc
    26161 gactactcgc gcaagcgggg cgagtggatc cccaacatgt acggcggccg cgagaacctc
    26221 gacgccgtct cccttctgca acgcgtgaac cacgacgcct accaggagtt tcccgacatc
    26281 gtgaccgtcg ccgaggaatc caccgcgtgg cccatggtgt cacgccccac gtatatcgga
    26341 ggcctcggct tcggcatgaa gtgggacatg ggctggatgc acgacaccct ggcctacttc
    26401 caacaggacc cgattcaccg caagtatcac cacaacaaac tgaccttccg cggactctac
    26461 gccttcacgg aaaatttcac gctgccgctt tcgcacgacg aagtggtgca cggcaaaggc
    26521 tccttgatcg gaaagatggc gggcgactat tggcagaagt ttgccaatct gcgcctgctc
    26581 ttcggataca tgttcactca gcccggcaag aagctgatct tcatgggcgg cgaaatcggc
    26641 cagtggtccg agtggaacca cgacggcagt gtggactgga ttctgctgac cttccccccg
    26701 caccagggaa tcaagcgctg ggtcagcgat ctgaacgctt tctatcgcgg cgagcccgcg
    26761 atgtacgaat atgatttcaa tcccgccgga tggcgctgga tcgacgccaa cgacaacgac
    26821 aacagcgtgc tcacctacct gcgctatggc aaggacgaga atgctccgct ggtcgtcgcc
    26881 tgcaacttca ccccagtacc gcgccgtggc tatcgcattg gagttcccaa gcctggccac
    26941 tggcgtgagg tgctcaacag caacgccacc atttacggcg gcaccgacga aggcaactac
    27001 ggcggagcca atagcgaggc gattgccagc cacggcttcg agcaatcgct gctgctcaac
    27061 cttcctccat tgggcatggt ggtgttcaag cagtaggagc cccaacaatc gagactttgg
    27121 gtgccccatt caaggacgcg aagcggcctt gggtgggggt tttctggctt gtcatcacga
    27181 gcgtagcgag ggatctgctt tccctgcacg tccccgaaaa ttccactccc atcgtaggtg
    27241 gatgtccaca cacctatttg tgtgatactt tacacataga ggcacactat ggcaaccaac
    27301 ctcgccattg acgacaagct gatcgaagaa gcccgccgca tcggcggcca cgtgacgaag
    27361 aaggatgcgg tgacggctgc gcttgacgag tacattcggc ggcgcaagca ggtcgatgtt
    27421 ttgaaattgt tcgggacggt ggaatacgag cgcggttacg atcacaaagc cagcaggcgc
    27481 cgtgaccgga tcgagcacga ccgatgaagc tgctggccga tacctcggtg tggtcgctgg
    27541 ccctgcgtcg gcgcgcgact gccacgttta gctccgagga tatccggctg aaggcagcct
    27601 tagcggaagc gatcagcgac gggcgggtgg tgatgatcgg gccgatccga caggagttgc
    27661 tgtcgggaat caaagagcag tcgcagttcg aaaaactgcg gactgcgctg gaagcttttc
    27721 gcgacgaagc gattaatact cccgactacg acgatgcggc gcggttttac aacttgtgca
    27781 gaagtagcgg agtggaatgc ggcccggttg atattctgat ctgcgccgta gcagtgcggc
    27841 gcaagtggag tgtgttagcc aacgacgcgc tcatgaaccg gtgcttgcag atcatcgtcg
    27901 cgcatcggcc ttaacgcgaa aatccaagct cacgcttcgc tcgaacgggg cacacagccc
    27961 ttattgaggg ggcgcccgcc tcaagatctc ttgagaccgt agaggcacgt agcggtctac
    28021 cgcctaacgt ttttattttc cctatgacat aaacccacat gacccgtcat ctaacagatg
    28081 cggcttaacg tcaagttggc caccgaggag gtgaacgatg tgtccattct gtttggcaac
    28141 catgggatta gtcgtcgccg ggacggtttc aaccggcggg ctagccgcat tagttgtgaa
    28201 agtttcgcgc aaaaagaacg gagcggaaga aatcgtcccg aatctgaata aaaaggagaa
    28261 atgagatggc accaagcaaa gacagtgacc aggaacacgg gaaagagcat ctcgcgatga
    28321 agacaccccc gatcgtgtcg acgcaggagt gggagaatgc gcgccagcaa ctcctgttga
    28381 aggagaaggc ttttactcgc tcccgtgacg cgctagccgc cgaacgccgg cggatgccct
    28441 gggtggctgt ggacaagaag tacgagttcg acggaccgaa gggcaaggta agcctgctcg
    28501 atttgttcga gggccgccct cagttgatcc tctatcgcgc cttcttcgag cccggggtgt
    28561 tcggctggcc cgagcacgcc tgccgcggct gctcctttgg cgccgatcag gtcgcccacc
    28621 tggcccatct gaacgcccgc gacaccacgc tcgcgtacac ctcgcgcgcg ccgcaggcgg
    28681 acatcgcgcg cctgaaggcg cggatgggct gggagatacc ctggtacacc atgaccgaca
    28741 gcttcgatgt ggacttcggc gtcgatgaat ggcacggcca caacgtgttc tttcgcgacg
    28801 gcgagcgcgt gttccgcaca tactttatca acgcccgcgg cgacgaggcg atggggacca
    28861 cctggagcta cctcgacata acgccgctcg gccgccagga gacttgggag gattcgccag
    28921 agggttaccc ccagaccccg ccgtacaagt ggtggaactg gcacgacaac tacgacgccg
    28981 aggcctcgcc tgacccgaag tgggtcgaca taacaacgga tcctaaaggc gccgccgccc
    29041 gaaggcgcga cgcagaggtt aaagcaacct aaagaagggc gaccacttcg ccgccttcga
    29101 gcagtctgca cgtttcagac aggcggtgag cgagtgcttc ggcaggaaag atgatgacga
    29161 agcatcggag gggagacagt gaggtcgagc gcgacctcca ggacaatgaa aacagcggcc
    29221 cggtcgatat cctgatttgc gccgtggcag tgcggcgcaa gtggagcctg ttggccaacg
    29281 acgcgcgctc atgaaccggt gcttgcagat gatcgttgcg caccgacaat cccggccaag
    29341 cttcgcttga acggggcacc cggcagttga ccggtgcaat agcaattgtg tgccgccggc
    29401 gagtaccagg aagacttccg cctgcgccga cgggtcggtt gcatagttga ttcccccgat
    29461 tcacgcttct tgcgccgtca cgatggatga ccaagaattg tgacatgagc gatccgcacc
    29521 gagagaaatg ggacattaaa agtgcgctcg taagaatttc gcactgggtc caaaccaccg
    29581 gacacatgaa tgaccagcgc tatagcggct gggatcactg ctgtcctaat tagccggcga
    29641 actgcctagc gactgctgaa atcaaagcac ataatccacc tttcggcgcc tgccgcaggg
    29701 caggttggcc gtccggaatc gcgatgctgg ataaagatgc tgtctaaatt cgactaccgc
    29761 ctgcctttcg atctactaat caagtggcca aaatagaagt ctatttgcga ttctgctgtc
    29821 caactattgc tccaggccta accgcaattg ctcgaacccg ggagccagca gcggcggtgc
    29881 ttgaaacgga ctgtcaatcc aagaccagag atcgcggtac acgaatagtt gttgccgcag
    29941 cagtgccacg agattcgaca acgaccaacc gaacctcgac cgcagttgca ggtatttgat
    30001 cagcaacatc gcgatcagcg ccgtccagat ctgcgtctgc agcgcgttct ccgaggttcc
    30061 cacaaaggtt ttcactcgca ggttctgctt gagcgcctta aaaaacaact cgatcgccca
    30121 cctgtccttg tacacggcag caatagtagc cgccgccaac ttcatgtgat tggtcaagaa
    30181 caccaggatt tgatcctgtt cctcgtcgta gtattccact cggcggaaaa agcattccac
    30241 ccccgcttgc gccagtttat agaagaagat cacctgatcc cgccggactc cctttcgctg
    30301 cgggatctcg cgctcttcca ccaccgcata gtcggcgttg tcctttaacc gggtcaccaa
    30361 atacactcca tcctgggtca agcgcgcaaa ccactcgtag tcggtatagc cgcggtcgat
    30421 cacccatacc gttccgggtg cgaactgcag ttgccgcgcc acccgcactt catgctgctt
    30481 gccctcggtc agcactgcat agctgggcag atgcccgtcg tgatccagca acagatgcag
    30541 cttcaccgcc cctttggtgc ggcgaaattg cgcccaatca aacaccgaca cgcacaggct
    30601 aatcaccgtc gcatccaaac tcagcagctt gtttttgaag cggaactttc gcttcgtctt
    30661 tcccggctgg ctggccacta cgctctgaca cttctccagc agttgctgaa agactgtctg
    30721 gtacagcggc cagggccggt gttcgttggc atacgccaga gtggagcgag caggcgctgc
    30781 gggtaatccc aagtgcttca attttccttc cgaggcggct aatccgccgc aaatttcacg
    30841 cagcgattgc gcatgcccca actgacaaaa cagcatcgcc acgaactgtc cccaactggt
    30901 gaaaccccgg gcgtggcgct cggctttggg cttccgcacc gccgattcaa attccagtcg
    30961 cggaaaaagc tgcaagattt gcgagaatat gctgcatact cgattcaagg ctgagcctcc
    31021 tagtcgatga ttggtttttt cgagaaa
//
LOCUS       DASRKK010000636         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1037220, whole genome shotgun sequence.
ACCESSION   DASRKK010000636 DASRKK010000000
VERSION     DASRKK010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037220"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..337)
                     /locus_tag="VFC15_15570"
     CDS             complement(32..337)
                     /locus_tag="VFC15_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZM11625.1"
                     /translation="MNADAVHFYFMTGRPSLVASTLRRRVSAYTRKDLVRGFKIGITN
                     NPPARFSNGYSDYDEMIVLYKSSSLDSVSEVERDLIDHNQEITKNRIAGGRNPSVRS"
     gene            complement(448..>2144)
                     /locus_tag="VFC15_15575"
     CDS             complement(448..>2144)
                     /locus_tag="VFC15_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM11626.1"
                     /translation="YRNSLVVENHFDWEDDQPLNIPLVDSIVYEAHVRSFTQHPSSGV
                     AHPGTYAGLIEKIPYLKKLGVTAVELLPVNEFEEGDTDRVNPVTGERLLNLWGYQPTA
                     FFAPDSAYSSNRADGEQVREFKQLVRALHQAGIEVILDMVFNHTAEGDDNGPTWSFRG
                     IDNSTYYILDPVTGKYVNFTGCGNTVNCNNPVVRDFIVDCLRYWVLEMHVDGFRFDLA
                     SILGRGMDGRVLKNPPLLESLAYDPVLANTKLIAEAWDAAGLYQVGTLPSWGRWAEWN
                     GIFRDDVRKFVKGDAGMTSALATRLLGSPDLYETSAREPYHSINFVTCHDGFTLNDLV
                     SYNEKHNLANGDNNTDGSDANYSWNCGVEGPTNDAAINELRARQRKNFAAILLFSHGV
                     PMILAGDEIARTQQGNNNAYCQDNEVSWLDWRMTESHAGLLRFFQQMIAFRKRCALLR
                     RDSFALHGEGGFHITWHGVKRAQPDWGQASRTVAMQLTQLHADGSRDDLHFIANAYAG
                     GLEFELPQIGEREWFRFADTALASPLDIAEEGLEFPLLSQESYLLHARSVAIFIAK"
BASE COUNT          429 a          606 c          645 g          464 t
ORIGIN      
        1 ttgatcgctg caagaccacc cgatctcgcc ttcaacttct tacggacgga ttacggccgc
       61 ccgctattct gtttttcgtg atctcctggt tgtggtcgat gaggtcgcgt tcgacttctg
      121 aaacggaatc aagcgacgaa cttttgtaga gcacgatcat ttcgtcatag tccgagtagc
      181 cattcgagaa ccttgcgggc gggttgttcg taatcccgat tttgaatccg cgcacaagat
      241 ctttccgggt gtacgcgctc accctccgtc gcaacgtgct tgcgaccaac gagggtctgc
      301 ccgtcatgaa gtagaaatgt accgcgtctg cgttcatacg gcctcaagtt tacaatacat
      361 tttcgttcgg cccgccgtgt ttcacaaatg gcaaacgagg cgttcccgga ctcttagacg
      421 ccggtctgaa gttttcctag ggcaggctca cttggcgatg aagatggcca cgctgcgggc
      481 gtgcaggaga tagctctcct gggacaagag cgggaactcc aagccctctt cggcgatgtc
      541 aagtggagaa gccagggcgg tgtcggcgaa acggaaccac tcgcgctcgc cgatctgcgg
      601 cagttcgaac tcgaggccgc cggcgtacgc gttggcgatg aaatgcaggt cgtcgcgcga
      661 gccgtcggca tgcaactggg tcaactgcat cgcgaccgtg cgcgatgcct gtccccagtc
      721 cggttgcgca cgcttcacac catgccacgt gatgtggaaa ccgccttcgc catgaagcgc
      781 aaagctgtcg cggcgcagta acgcacagcg cttgcggaac gcaatcatct gctggaagaa
      841 gcgcaacagg ccggcgtggc tctccgtcat acgccagtcc agccagctca cttcgttgtc
      901 ctgacagtag gcgttgttgt tgccctgctg cgtgcgtgca atttcgtcgc ccgcgagaat
      961 catgggaaca ccgtgcgaga acagcaaaat ggcggcaaag ttcttgcgtt gacgcgcgcg
     1021 cagttcgtta atcgcggcat cgttcgtggg gccttcgacg ccgcaattcc aactgtagtt
     1081 ggcgtcggaa ccgtcggtgt tgttgtcgcc gttggcgaga ttgtgcttct cgttgtacga
     1141 aaccaaatca ttcaacgtga agccgtcgtg gcaggtcacg aagttgatgc tgtggtaagg
     1201 ttcgcgggca ctggtctcgt agaggtcggg acttcccagc aaacgggtgg cgagtgccga
     1261 ggtcatgcct gcgtcgccct tcacgaactt gcgcacatcg tcgcgaaaga tgccattcca
     1321 ctccgcccat cgtccccagg aaggcagagt gccgacctgg tacagcccgg cggcatccca
     1381 ggcttcggcg atcagtttgg tattagcgag cacaggatcg taggccagac tctccagcag
     1441 cggtggattc ttgagcactc gcccatccat tccgcggccg agaatggaag cgagatcgaa
     1501 gcggaagccg tcgacgtgca tttccaggac ccagtagcgc aggcaatcga cgatgaagtc
     1561 gcgcaccacg ggattgttgc aattcacggt gttgccgcaa ccggtgaagt tcacgtattt
     1621 gccggtcacc ggatcgagaa tgtaataggt ggagttatcg atgccgcgga aggaccacgt
     1681 cggcccgttg tcgtcgcctt cggcggtgtg gttgaacacc atatcgagaa tgacctcgat
     1741 gccggcttga tgcagggcgc gcaccagttg cttgaactcg cggacctgct cgccatcggc
     1801 gcggttcgat gagtaggcac tgtcgggcgc gaaaaacgct gtaggctgat aaccccaaag
     1861 attgagcaga cgctcgccag taactgggtt gacgcgatca gtgtcgcctt cctcaaactc
     1921 gtttaccggg agaagttcga ccgccgtcac gcccaatttt ttgagatagg ggatcttctc
     1981 gatgagccct gcgtaggtcc cgggatgcgc gaccccggaa gacggatgtt gggtgaagct
     2041 acggacgtgc gcttcgtaga cgatgctgtc caccagggga atgttgagcg gctggtcgtc
     2101 ttcccaatcg aagtggttct ctaccaccag actgttgcga taag
//
LOCUS       DASRKK010000637         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_548438, whole genome shotgun sequence.
ACCESSION   DASRKK010000637 DASRKK010000000
VERSION     DASRKK010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548438"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFC15_15580"
     CDS             complement(<1..1044)
                     /locus_tag="VFC15_15580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM11627.1"
                     /translation="MDMFERPQHSLRELIQLSPLAIEELDLQGNVKLWNSAAERMFGW
                     EESEVLGRPYPAIPSESRAEFEDEVLQLLGGATIQGNEVCRLCKDGRRLTARLWAAPI
                     HNQRNEIIGIAKILEDITIQKGAEERLLLQATVLQATANSIVITDNQGTILWVNQAFS
                     QLTGYSSGEAVGKNPRLLKSGEHDSAFYRNMWAAITSGNIWNGEVINRKKDGSLYTEE
                     MTITPLHIGTGGITHYVAIKQNVTLRKLAEDGLRRAEEKYRLIFQNAPIGIFQATPEG
                     RSIIVNPALARMHGYDSPEQLMAEVSNVGHQLFVDPNALQEIARLLEKEPVLRDIELE
                     IYDKGGKRRWLSAN"
     gene            complement(1807..>2501)
                     /locus_tag="VFC15_15585"
     CDS             complement(1807..>2501)
                     /locus_tag="VFC15_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM11628.1"
                     /translation="AQVATATIVALVLLFLTKPLQYLPRCVLGAIVFFIAIRLIDVRA
                     LRDIQRESPGEYGLALLTAVIVVLVGVEQGILLAMALSLLRVVRHSYRPHTGVFVKVE
                     EKAWHLVPALPGAVTQPGLVIYRFGAALFYANASLFAEEVRGLVGPVSSPVRWLVVDA
                     EAITNLDYTAAQMVRQLHQELVPRGFVLALARVSSSLKADLKRHRLMEVIGPAQIFDR
                     LHDALAAFTELR"
BASE COUNT          523 a          721 c          674 g          583 t
ORIGIN      
        1 gttcgctgac aaccacctcc ttttaccgcc tttgtcgtag atttccaatt cgatgtcgcg
       61 cagcacaggc tccttttcca gcaagcgcgc gatttcctgc agcgcgttgg gatcgacgaa
      121 caactggtgt cctacattcg aaacttccgc catcagctgt tcgggagaat cgtaaccgtg
      181 catgcgtgcc aacgcgggat tgacaattat ggaacgaccc tctggagtgg cctgaaagat
      241 tccaatgggg gcattttgaa aaatcaagcg atatttctcc tcggctcgac gcaacccatc
      301 ttcggcaagc ttccgcaaag ttacgttttg tttgatcgca acataatggg tgatcccgcc
      361 agtgccgata tgcagtggcg tgatcgtcat ctcctcggtg tagagacttc catctttctt
      421 gcggttaatg acttccccat tccagatgtt tccggacgtg atagccgccc acatattcct
      481 atagaacgca ctgtcatgtt cacccgactt caggagccgg ggattcttgc ccacagcttc
      541 tcccgagctg tagccggtga gttgtgagaa tgcctggttg acccacagga ttgtgccttg
      601 gttgtcggta atcacgatgg agttggcggt tgcttgcaac accgtagcct gcaatagcag
      661 gcgctcttcc gctccttttt ggatggtgat gtcttcgaga atctttgcga tgccaatgat
      721 ctcgttccgc tggttgtgaa taggtgcggc ccagagtctc gcggtaagcc gccggccatc
      781 tttacatagt cgacatacct cgttgccctg gatcgtcgcg cccccgagga gctggagcac
      841 ctcatcttcg aactcggccc ggctttctga cggaatagcg gggtacggtc gacctagcac
      901 ctcgctttcc tcccagccga acatccgctc agcggcgctg ttccacagct tgacattgcc
      961 ttgtaggtcg agttcctcga ttgcgagggg agaaagctgg atgagttctc tcagggaatg
     1021 ttgcggacgc tcaaacatat ccattacact ttcgattgtt cagtttcccg gttatcgcac
     1081 gcgatactcc ggactattat cgaaaacgta ctctttttct aaggtattgt aagtgtatgg
     1141 cgaaactatg gcaggtcaag gaaaaaccgt aattttcttg ctttgctgcc agattctcga
     1201 gtcggaactg gatggcacag cgccctggat gtccacgaaa gactgatgcg gacacttggg
     1261 aattccttga tggcgggttg atctcgttaa tgaagatatc ttcggtgaag gccttggcga
     1321 ctcgctcgag gtctcgcccc ggcggcgtac caacatagtc agcatggtca gattattata
     1381 tccgtcggcg acggggacga cacgcccgac gggacgagag gacgccgact gatttcgccg
     1441 tctgtgatca gctccgcgat gttgggtagg gacaaggaag tgatggcgga gttcttcccg
     1501 gctgctttct tgtctactct gccggttcga ttgcgggcgt taggcctgca gcgatcctgc
     1561 aattcatcgc gatgtttcgc cgctgccgct tcgcgccttc tgctttctga aacaaagaac
     1621 cgaccaccat caccatcgac aaccgtttgg gctgccaaac catccttgct ctgtcactta
     1681 acctcgtgct ctccagccgt ctgctccgcc gataagctac ctcgacaatt gcgggccccg
     1741 aggctctgtt ctgatcgaga tctcgtttga tgcagattgc ctctccgatg taacttgcaa
     1801 ggtctattac ctgagctctg tgaatgctgc cagcgcatcg tgcaggcggt cgaagatctg
     1861 cgctgggcct atgacctcca tcaagcggtg ccgctttagg tctgctttca gcgaggacga
     1921 cacgcgtgcc aacgccaaca cgaaaccgcg aggaaccagc tcctggtgaa gttggcgcac
     1981 catctgcgcg gccgtgtaat ccaggttcgt gatcgcctca gcatcaacca ccagccagcg
     2041 cacgggcgaa gaaactggcc caactaggcc gcgaacctcc tccgcaaaca ggctggcatt
     2101 ggcgtagaac agcgccgctc cgaagcgata gatcaccaga ccgggctggg tgacagcacc
     2161 gggcaaggcc ggcaccaaat gccatgcttt ttcctctact ttgacgaaca ctccggtgtg
     2221 aggacggtag ctatgccgca caacccggag cagggacagc gccattgcca gcaaaatgcc
     2281 ttgttcgacg ccgaccagca ccacgatgac agccgttaat agggccagac cgtattctcc
     2341 ggggctctcg cgctgaatgt cccgcaaggc gcgcacatcg atgaggcgga tagcaatgaa
     2401 gaatacaatc gcgcccagca cgcagcgcgg aaggtactgc aaaggcttgg tcagaaagag
     2461 cagcacgagt gccactatcg tcgccgtggc cacctgcgcg a
//
LOCUS       DASRKK010000638         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_548664, whole genome shotgun sequence.
ACCESSION   DASRKK010000638 DASRKK010000000
VERSION     DASRKK010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548664"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..160
                     /locus_tag="VFC15_15590"
     CDS             89..160
                     /locus_tag="VFC15_15590"
                     /inference="COORDINATES: protein motif:HMM:NF044419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11629.1"
                     /translation="MGSDIEYADEVTMQKAMEGRREL"
     gene            complement(512..1066)
                     /locus_tag="VFC15_15595"
     CDS             complement(512..1066)
                     /locus_tag="VFC15_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11630.1"
                     /translation="MNRELAISAVMLFAAMITSSAQEPAPSTETAPLHTRTDFSFTVD
                     APFDQVAPLFGANEERKWSAGWNPQFIYPTPARDQQGMVVKVAPGQFTSVWVNTALDL
                     AAGHIQYAYVLNDAMAVLIDIHLTRESAQKTGVTVVYERTALLSEANEHVQHFTKGDA
                     GAGKEWGDAINNYFAELRATGSQR"
     gene            complement(1063..1329)
                     /locus_tag="VFC15_15600"
     CDS             complement(1063..1329)
                     /locus_tag="VFC15_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11631.1"
                     /translation="MKQQSAAIVVDRVQTGVRMEKRLLKVLKGLAELKDLTLGDLLEG
                     IVLHAFEGKAPFSPATLKEIEQLRKIYGLTLKATDSHKLKERQG"
     gene            complement(1626..2666)
                     /locus_tag="VFC15_15605"
     CDS             complement(1626..2666)
                     /locus_tag="VFC15_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM11632.1"
                     /translation="MKTSEVLHAWGAILTGRRPSLSIELTRECPLRCPGCYAYEDAHL
                     GTAGITLRQLSDSKGSALVQGVLRVVEEHRPLHLSLVGGDPLVRYRELLQLLPELDRR
                     GIHVQLVTSAFREIPAEWNALSRLGIVVSVDGLQPEHDVRRKPATYERILKCIAHSRV
                     SIHCTITSQMMHREGYLEEFVKFWSLRKETKRIWFSIFTPQKGAVDPEILTVQERTRA
                     IAELLRLREVYPLLDMYASAIKEFAHPPASPRDCIFASTTTTISADLKTRVTPCQFGG
                     DPDCSQCGCIASMGLAAVGHHQLLPGITTGQIFTVSQLIGRSVNALRSFGQSNEAVVE
                     PEVEVEEETRVA"
BASE COUNT          567 a          805 c          876 g          617 t
ORIGIN      
        1 cgtcgaaggg gaagccacgg cgacctatct ttcgcggttg ttcaagtcgg ctacggtaaa
       61 ggtcacccgc atcgccaccg gcgttcccgt gggcagcgat atcgagtacg ccgatgaggt
      121 caccatgcag aaggcgatgg aagggcgaag ggagttataa gggtagacgc tcttcgctgt
      181 tcgccgctcg ctcttcgcca gagcgcctca aacattgcgt tgtcatcctg agcaacggac
      241 ccgctggggc aggttgaaac aggaatgaca actctgcaag atcgtccggc taagtccttt
      301 gtttgcgttg ttgtcattgg gagcgttaca tgggcttgcc gccgactccc ggggatgaaa
      361 ataaccacgg agggcatgga gatcacggag aaaacttctt gaaaacaaac tccgtgtcct
      421 ccgcggtgaa tgattttcga cggagcgaga agcgcagcga ctgcgtccgg tcgcaaggcg
      481 acgttacttc gctcagaatg acagccgaat gttatctctg cgaacccgtc gcacgaagtt
      541 cagcgaagta attgttgatc gcgtcgcccc actccttgcc tgcgcctgca tcgcccttgg
      601 tgaagtgttg aacatgctca ttcgcttcgg acagtaacgc ggtgcgttca tagacgaccg
      661 tcactcccgt cttctgggcg ctctcgcgcg tcaagtgaat gtcgatcagc acggccatgg
      721 cgtcgttcag cacgtaggcg tactggatat gtccggccgc caagtcgagc gccgtgttga
      781 cccacacgct ggtgaactgt ccgggcgcaa ctttgaccac catgccttgc tgatcgcgcg
      841 caggagtggg atagatgaac tgtggattcc agcccgccga ccattttcgc tcctcattcg
      901 cgccaaatag cggcgcgact tgatcgaagg gcgcgtcaac cgtaaagctg aagtcggtgc
      961 gggtgtgaag cggcgcggtt tcggtggaag gcgccggctc ctgggcgctg gacgtaatca
     1021 tggcggcgaa caacataacg gcgctgattg ctagttcgcg attcatccct ggcgctcctt
     1081 cagcttatgg ctgtctgtgg ccttgagggt gaggccatag atttttcgaa gctgttcgat
     1141 ctccttcaag gtcgcgggag aaaagggcgc cttgccctca aaggcgtgca gcacgattcc
     1201 ctccagcaaa tcgccgagcg tgaggtcttt cagttcggcc aatcccttga gtaccttgag
     1261 caggcgtttt tccatgcgga cgccggtctg aacccggtcg acaacgatgg cagcagattg
     1321 ctgtttcatg gtaacaatgt accaaagtac cataataaat acaataaggg gcgacctgga
     1381 aagcgcgacc caaagtcatt tcgcagtgcc catacttaag gaggtcgtgc caggactgtg
     1441 ccgtccctga agggactcgg tcttattttc ccactctacc cagcgctgaa gcgctgggct
     1501 aagctctttc gccccattcg gggctggttc ttgggtgctt cctcccactg cgcaaaccga
     1561 tactaggtcg cgacacggtc tctgacggct ggctctacct gaacttcgcg tggctcagag
     1621 ttttcctagg cgacccgggt ctcttcttca acttcaacct cgggctcgac gacggcttca
     1681 ttcgattggc cgaaagaacg cagcgcgtta acgctgcggc cgatcaactg cgagacggtg
     1741 aagatctgcc ccgtggttat gccgggaagg agctgatggt ggccaaccgc ggctaggccc
     1801 atacttgcaa tgcagccgca ttgcgaacag tcggggtcgc caccgaattg gcagggggtg
     1861 acacgggttt tcaggtcggc tgaaatggtg gtcgtggtgc tcgcgaagat gcagtcgcgg
     1921 ggcgaagccg gcgggtgcgc gaactccttg atcgccgacg catacatgtc gagcagcgga
     1981 tacacttccc gtaatcgcaa cagttcggca atggcgcgtg tccgctcttg cacggtcagg
     2041 atctccggat cgacggcgcc tttctgcggc gtgaagatac tgaaccagat ccgtttggtc
     2101 tcttttcgca ggctccagaa cttgacaaac tcttccaggt aaccttcacg atgcatcatc
     2161 tggctggtga tggtgcaatg aatgctgacg cggctgtgag cgatgcactt caggatgcgt
     2221 tcataggtcg ccggcttgcg gcggacgtcg tgctccggtt gcaatccgtc cacggagacc
     2281 acaatgccta aacgcgatag tgcattccac tcggcgggaa tctcgcggaa cgcactggtc
     2341 accagttgca cgtggatgcc gcgacggtcc aactcgggca gcagttgcag cagctcgcga
     2401 tagcggacca gcgggtctcc gcctacgagg gagaggtgca gcgggcggtg ctcctcaacc
     2461 acgcgaagaa cgccctgtac cagggcgctg cccttgctgt cggagagctg gcgcaacgtt
     2521 atgccagccg tgcccaggtg tgcgtcttcg taggcgtaac aacccggaca gcgaagtgga
     2581 cattcgcggg tgagttcgat ggacaatgag ggacggcgtc cggtcaggat cgcgccccac
     2641 gcatggagaa cttccgaagt tttcatcagg tctctggtag gtaccgtttc tccattggat
     2701 gcctggcccg gcgactaggg tgcgcagcag attgttgggg agaggtcctg tttatcctcg
     2761 caattttgcc agcaggtcag aaggatggac gggcttgccg agaatttcga actcgtgacc
     2821 ctgggcgcga gcattttcca gcaggtcggc ggtggccgct tgtcc
//
LOCUS       DASRKK010000639        17241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1233583, whole genome shotgun sequence.
ACCESSION   DASRKK010000639 DASRKK010000000
VERSION     DASRKK010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233583"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1029
                     /locus_tag="VFC15_15610"
     CDS             304..1029
                     /locus_tag="VFC15_15610"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZM11633.1"
                     /translation="MEKLSAADRLFVLTGAGVSAESGLPTFRGVNGIWRGYRVEDVAT
                     PGAFAADPVMVWQFYSERRQRHGTVQPNTAHFALAGIERALQDRFFLCTQNVDSLHEQ
                     AGSQRVVHMHGRLMQSRCSRSSCRTLPLDDPNLYRTGSEILNCPKCGAMVRPHICWFG
                     EVPFHMDLILQQLDAATVLLTVGTSGVVEPAASFVRMARRNRARTIYVGPEAPSNSEY
                     FDEVLLGKAGEVLPGLVEDLVNG"
     gene            complement(1019..2899)
                     /gene="feoB"
                     /locus_tag="VFC15_15615"
     CDS             complement(1019..2899)
                     /gene="feoB"
                     /locus_tag="VFC15_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZM11634.1"
                     /translation="MTCHPVALPQDEAPDTSYRTVAVVGPPNSGKTTLFNRLTGLRQK
                     VGNFPGVTVEHHTGYIRDGAGEEVALIDLPGIYSLTAKSEDEKVAIAVLQGNMPGMNR
                     PDAVIVVLNSTNLHSHLVLAGRVIALGLPTLVLLNMADELHKQDGGVDVLSLARQLGT
                     PVALVSATTGAGLEAVTNFLSGASPALEPMELPVIGSTRSVREWAVRVGENANYRRPA
                     TPIWTSRLDNVFLNRTWGPVIFAVVVIAVFQTIFAVGQPLSNLLQKFLDVTGSRIGTV
                     LPDGMFRSLLINGVWKGTGSVLVFLPQILLLFLVIGILEDSGYLARAAVIADRTMSRV
                     GLNGKSFIPLLSAYACAVPAIMATRTIESKRDRIATILIAPFMTCSARLPVYTMVIAA
                     FLPNRPLLGPFLGTRAVVMLSLYLLGFLMAVLTARLLKSTVLRSKDAPFILEMPPYRW
                     PKFSSLMMRLVDRGKAFLYRAGTVIMLVSLLLWAATNFPMHNGQPPAIQDSVVASLGH
                     AIEPVIRPLGFDWKIGVGLVTSLAAREVIIATLGTLNGIDAESHSFDLQNALRHELTI
                     PAAIALLVFFAFAMQCMSTIAVVRRETNGWKWPAIQFTYMTVVAYVSAFAAFNIAGYF
                     IH"
     gene            complement(2918..3214)
                     /locus_tag="VFC15_15620"
     CDS             complement(2918..3214)
                     /locus_tag="VFC15_15620"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZM11635.1"
                     /translation="MIFNFIFGRKQRGHHKSGALADLRVGERAILREFELDHPIAEHL
                     MNLGFVPGLEVTVERSGPGGDPRVYRVEGTEVAMRREVSRHIHVSLVGAPAFGD"
     gene            3542..4705
                     /locus_tag="VFC15_15625"
     CDS             3542..4705
                     /locus_tag="VFC15_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM11636.1"
                     /translation="MLGLSEIASRIVQSTIRTMSVECEKVGGVNLAQGVCDTEVPAIV
                     RQAAENAIEAGYNQYTRADGIASLREAIAKKLQRQNGIVANPNSEILVTSGVTGGFDS
                     ACRALLNPGDEVVLFEPYYGYHYATLVGQYCRPVLVSLDPPDYPLVADRLAAAITAKT
                     RGIVVNTPANPSGKVFSRVELEQIAAVARQHDLFLFTDEIYEHFVYDGERHISPASLP
                     GIADRAITISGFSKVFSITGWRVGYLHASAQWIPAISYFHDLTYVCAPSAFQHGAAAG
                     LEQLPDSFYANLIQDYQLKREMICAALRVAGMEPSVPAGAYYVLADASVVPGADSVEK
                     AMNLLRLTGVASVPGDAFFQGGRGDHLLRFCFAKPDNDLANACERLAGLPRGA"
     gene            5084..7684
                     /gene="glnD"
                     /locus_tag="VFC15_15630"
     CDS             5084..7684
                     /gene="glnD"
                     /locus_tag="VFC15_15630"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZM11637.1"
                     /translation="MSTPTFDPALRSFYKEGSDRVRRDFEANGSGRSCVQDRTQIVDK
                     LLLHLWEQQHALCSNTGFALVALGGFGRRALYPHSDIDLLFLCENETLRTRAKDPIRA
                     ICQELWDTGLRVSPTTRTIDDCGRFDQDNVEFTISLLDCRFLIGDEKLFARLHDKTLP
                     QLVARESGILIQRLTEVTKTRHVKFGKTIFHLEPNLKDGPGGLRDFHVTRWIAMIAAL
                     EANRKWPESLHLQQNSDDDDVLEAMEFLSSARCYLHYRNNRDENIVTWEAQEDLASRG
                     IGSGMGPLTSAEWMRLYFRNAKATYRNATQLLEQTSANRSSLVRSFRRWRSRASNDEF
                     SIVDRRLYIQQAAGARDAEVVLRLFVFMARHGVSLAMETERRLNNARRALRDTMPQDA
                     RLWACLRELLVQPHAAEALRSMHSLRLLAMALPEFETIDSLVLRDLYHRYTVDEHTFL
                     AIDVLHRLRDNEVEWLQPFAGLFTELERPELLFLALLFHDTGKGLAGDNHIHGSLQLA
                     LAAVERLGLSKEDAQTVGFLIAGHLEMSSTLRRRDIYDPETVRELAGKMETPERLKML
                     TLLTLADIRAVNPDALTPWKAENLWQLYIATANYFTRSVDEQRFHAEVCTEQVERIAA
                     LLPKNRSQLLKFLDGLPQRYLMSHAPAQVIEHFEMASRLGTEPVQLGLRRTREQHELT
                     VVTEDREGLFATLTGILYGWGMDITKASAFSNRAGVIVDSFYFKDRFRTLELNPSERE
                     RFKKSVIDVLSGDSPLDGLLEPRLRADTKAPKLKIETRLRYDDECSPGSTLLEVVTQD
                     RPGLLYTISSTLAAENCGIEVALIDTEGEMAHDVFYLTSGGMKLTRDQQRAVEWALTT
                     ELGESLPSSW"
     gene            7907..9172
                     /locus_tag="VFC15_15635"
     CDS             7907..9172
                     /locus_tag="VFC15_15635"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HZM11638.1"
                     /translation="MTALDIRHVLADWQIIAAWIVFLASYFVFAFGRLPGTKIDRPAM
                     AVVGAVLMFVFRILTPQTAIGSVDFGTLVLLFAMMLIVASLHLAGFFDWVGNRVILHL
                     APRQLLPGVIFTSGILSAFLVNDVVCLFMAPLVLGICKRMGLRPLPYLLALATASNIG
                     GVATITGNPQNMLIGTVSGIAYRDFLAHLGPVAVIGLFLDWAILHWAYVRGNIAAVDP
                     QPEISEANVVSAPANRRMLWPVMITIAVLAGFLLGFHPPLVAAAGGALLLVRRTREPR
                     DVYGDVDWSLLVLFLGLFLIIGGAEQEGVTSQLLRAAERLNLHHSLVFVSMVALLSNL
                     VSNVPAVMLLKGLIPRFQDPHTMWLMLGMSSTLAGNLTITGSVANIIVVEKARTVTHI
                     SFLDYMKTGVPVTIATLAVGLLCLNFVRY"
     gene            complement(9189..10163)
                     /gene="thrB"
                     /locus_tag="VFC15_15640"
     CDS             complement(9189..10163)
                     /gene="thrB"
                     /locus_tag="VFC15_15640"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZM11639.1"
                     /translation="MAERSDSSALRFRMFLPATSANLGPAFDAAALALDFGLRVEAIL
                     AAEFSIDAHGRNADQCGKMPGNLIVDTYTRLLEREGKHATPLAIRLDNDIPLGMGCGS
                     SAAAVLASVAMAAYFGALDWDGERIVSEASTCEGHPDNVAACWHGGVVLARTASVVRN
                     ASVPTISVLPIPVAVDWPLLIAIPENPLATSEARRVLPRLYSRVDLVHNVQSAMLLVL
                     AFSQGRGEWLNEALSDRVHHPYRAQLCPLLPALTALAGAEGVNGVALSGAGPSVLMFL
                     HEDADRDEVGARVAAHLIDKGLTAELRFTAISAIGAKDTFPISATATR"
     gene            complement(10156..11535)
                     /gene="thrC"
                     /locus_tag="VFC15_15645"
     CDS             complement(10156..11535)
                     /gene="thrC"
                     /locus_tag="VFC15_15645"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZM11640.1"
                     /translation="MGFLHADSVTAAKNYRVRCISCGEATAALAVDFRCGNCGDLLEV
                     EFINWSDGSALDPAKLKSLWRDRRTSNLPLDTSGVWRFRDVLPELDDWSSAITLREGN
                     TPVYDLPGCARSAGIERLRAKHQGMNPTGSFKDTGMTVAISFARQNKFRWVACASTGN
                     TSASMAAYAARGGMRSLVVIPDGKIAWGKLSQSLDYGALTCQLRTDFDGCVRVLNDVI
                     RRYPIYLLNSVNPFRLEGQKTAAIELLEQLEWQVPDHVIVPGGNLGNNSALGKAFLEM
                     KRLGVVQSVPKISVIQAEGANPLYRTVHEKFGKELVTVEADTLATAIRIGNPASWMKS
                     LRVIEATEGCVEQVSEAEIAVAKAEIGADGVGCEPASAVTLAGLKKLVKSGFVQRSDS
                     VVLVLTGHLLKDPEYTIKFHRGDLSVPRMADNEFHALTAQQRKAPIVLDADADAVLRA
                     LVAEEKRNG"
     gene            complement(11732..12481)
                     /locus_tag="VFC15_15650"
     CDS             complement(11732..12481)
                     /locus_tag="VFC15_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11641.1"
                     /translation="MPIVVDPELKDKTTNPEAPTYKERELFAAEIEMESKSSNFGPLV
                     MVLVLVLAVGGTIFYFVKTSREVLTAPVATNSINEILKSQAGGNIHFSVGTIVSSVNE
                     KPMDPHYKLLAKAGILTIKPKSWNSIYATMTPAGEKLLSEIKGVDKGKNSDGNTNYVV
                     PLAERKIVSIDKITMIKPHLARVDYTWKWDANRLGKEFDASSDMVHTFNTWDRGTLIK
                     DYGVDFYNGAPTKASVVLAETKDGVWKPYVE"
     gene            complement(12736..13866)
                     /gene="modC"
                     /locus_tag="VFC15_15655"
     CDS             complement(12736..13866)
                     /gene="modC"
                     /locus_tag="VFC15_15655"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HZM11642.1"
                     /translation="MSSAEIQIPARELSVSLHKRLGGEGREDTSLQLDVRFMIASGFT
                     ILFGASGAGKTTVLDCIAGLQEPDSGHIAVGDIVLFDSEKGIELPARSRNIGYLFQTL
                     ALFPHMTVRQNIAYGLSMLDRHERENRVSEIAESFGISGLFDRRPFEISGGERQRVAL
                     ARALVTRPRALLLDEPLTALDAGTKTRILDDLRLWNDRHAVPILYVTHQREEVYALGN
                     RVLVLEAGKLIADGTPHDVLSRPQFESVAQLAGFENIVECSVIASHAEQGTMTCRIAG
                     TEVSLEVPLSRVDTARPVRVGIRAGDILVATSLPQGLSARNVIAGFIVLLRQQDVTVI
                     AEIDCGTRFVVHLTPGACQALNLEVGKQLWLVVKTYSCHILQ"
     gene            complement(13863..14540)
                     /gene="modB"
                     /locus_tag="VFC15_15660"
     CDS             complement(13863..14540)
                     /gene="modB"
                     /locus_tag="VFC15_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZM11643.1"
                     /translation="MILQALALTLRLAILVSLILMAIGLPLGYWLASSRWRGKFVVEA
                     IVSLPIVLPPTVLGFYVLVAIGPLSPFGRWYHSITGSSLAFTFRGLVVGSVLYSLPFA
                     VQPIAAAFASVDRKLIEASAMLGASRWRTFWRIVLPLGFPGVLTGFVLSFAHTLGEFG
                     VVLMIGGNIPGATQTVSILIYDQVQSLNYAVANRTALVLLLLCFALLTLLYGLRRKSK
                     WVLWGWQ"
     gene            complement(14537..15289)
                     /gene="modA"
                     /locus_tag="VFC15_15665"
     CDS             complement(14537..15289)
                     /gene="modA"
                     /locus_tag="VFC15_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZM11644.1"
                     /translation="MLLIFVAFAFRTATAEPITVAAAADLNYAMNELAGRFETATGTK
                     VTLSFGASGNLFSQIQSGAPFDLFFSADEDYSKKLVNTGVADASSLRIYALGHLVLWV
                     PNNSSFDPQKLQMELLNQPGVTRIAIANPEHAPYGRAAMTAIEHYGLIDKLAGKLVFG
                     ENVSQAAQFVQSGNAQAGLIAMSLAKSPAMESAGRFWVLPSDSYPELKQAAVIVSASK
                     HKKAAQAFMDFVLSPEGAATLRKYGLTPPAKP"
     gene            15567..15965
                     /locus_tag="VFC15_15670"
     CDS             15567..15965
                     /locus_tag="VFC15_15670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM11645.1"
                     /translation="MIRIEVTLNADPTAVSPVVEGIMEMARRMDCAAGKEFEIETALR
                     EALANAILHGCKNDPTKKVQCCVSCDEEHGLLIVVKDPGTGFDPVQVPDPLVGENVYS
                     NHGRGIFLINQLMDEVRFERNGTEIHMRKY"
     gene            complement(16011..17186)
                     /locus_tag="VFC15_15675"
     CDS             complement(16011..17186)
                     /locus_tag="VFC15_15675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZM11646.1"
                     /translation="MASVTTTTSELAGPGSARTSTARFYHPELDELRFFAFLMVFLHH
                     ALPHDPTFWTNLGSPVFLARVVAGVGATGAFGVSLFFVLSSYLITELLLREKQLIGTL
                     DIRSFYIRRILRIWPLYFAFLALAVALQWIVPGQHVTLRAGVWFSFLAGNWFIVFHGF
                     PSSVIFPLWSVSIEEQFYITWPAVVRKVSETGMLLFAGGLLAAATVARIYLGMDHALE
                     GDVWCNTFVQLDPIAAGILIAVLLKGEIPRLSRLARVAMMLAGITALAFGSLYFGIKN
                     DPLTTARIVLGYPSVAIGGALLLLSVLRSRTSGGNRALIYLGRISYGLYVFHVLGLLI
                     SDHTVPDQTASLFRYSLRVGVALAATIAMAAVSYRWLETPFLSLKQRFTHVLSRPGG"
BASE COUNT         3657 a         5157 c         4888 g         3539 t
ORIGIN      
        1 tgccccaagg gatcgaggtc cgcttgcagg taaccccaac ggcgaaacga atccattacg
       61 cgttccagct tttcgctgtc aacgttcatc agtacagtgg acaaggatga aaactcccaa
      121 gccgcggagc cactcggcaa ttccgggcct gcgtatttag gatgtgctct ttatagattc
      181 accaccgagt ggacggatgc gtccacttgg tccggaatcg gacacgggtg gcaaccaaac
      241 cacttagtat agcgaaacag attgtggttt tgaaaactac gccgggactg agctgggggc
      301 acgatggaaa aactgtcggc ggcggatcgc ttgttcgtgc tcaccggcgc gggagtcagc
      361 gccgagagcg ggctgcccac atttcgtggt gtcaacggca tctggcgcgg ttaccgcgta
      421 gaggatgtcg caacccccgg ggccttcgcc gccgacccgg tgatggtttg gcaattttat
      481 tccgagcgtc ggcagcgaca cgggacggtc cagccgaata ccgcgcattt cgcgttagcg
      541 ggcatcgagc gcgcactgca ggaccgcttc ttcctgtgca cgcagaatgt cgattcactg
      601 cacgagcagg cgggatcgca gcgcgtcgtc cacatgcatg gcaggttgat gcagtcacgt
      661 tgttcgcgat cgtcctgccg cactcttccc ctcgacgatc ccaatctcta tcgcaccgga
      721 tcggagatcc ttaattgccc gaagtgtgga gcgatggtgc gtcctcacat ttgctggttc
      781 ggcgaagtgc cgtttcacat ggacctgatc ctgcagcaac tggacgctgc tactgtgctg
      841 ctcacagtgg gaacgtctgg cgtggtcgaa cctgctgcca gttttgtgcg gatggcacgc
      901 cgcaatcgcg cacgcaccat ctacgttggc cccgaggcgc cgtcgaacag cgagtatttc
      961 gatgaagtgc tgctggggaa ggcgggcgaa gtgttgccag gcttagtcga ggatctggtt
     1021 aatggatgaa atatccagcg atgttgaacg cggcaaatgc tgacacatac gcgacgaccg
     1081 tcatgtaagt gaactgaatc gccggccact tccatccatt ggtctcgcga cgcaccaccg
     1141 caatggtgga catacactgc atggcgaaag cgaagaacac cagcagcgcg atcgcagccg
     1201 gaatcgtcaa ctcatgccgc aacgcattct gcagatcgaa cgagtgcgac tcggcatcga
     1261 tgccgttcag tgtgcccagc gtcgcgatga tcacttcccg tgccgccagc gaggtcacca
     1321 gtccaacgcc gatcttccaa tcgaagccca atggccgaat cactggctca atcgcgtgcc
     1381 cgagcgacgc aaccacgctg tcttgaatcg ccggaggttg tccattgtgc atggggaaat
     1441 tggtggctgc ccagagcagc agcgaaacca gcatgatcac cgttcccgcc cggtacagga
     1501 aggcctttcc gcgatcgacc agccgcatca tcagggaaga aaactttggc cagcgatacg
     1561 gcggcatctc cagaatgaag ggagcgtcct tgcttctcag caccgttgac ttcagtaggc
     1621 gcgccgtaag tactgccatg aggaatccga gcaggtacaa gctcagcatg accactgcgc
     1681 gggttccgag aaacggtccc agcaaaggac ggttcggcag gaacgccgcg atcaccatgg
     1741 tgtaaaccgg cagacgcgcc gagcaggtca tgaatggcgc gatcaggatg gtagcgatgc
     1801 gatcgcgttt gctctcgatg gtgcgcgtcg ccatgatcgc aggaactgcg cacgcgtagg
     1861 cggagagaag cgggataaaa ctcttgccat tcagtcccac gcgcgacatg gtgcgatcgg
     1921 cgatgaccgc cgcgcgcgcc aggtagcccg agtcttccag aatcccgatc acgagaaaca
     1981 acaatagaat ctgcggcaga aaaaccagca ccgagccggt ccccttccac acgccattaa
     2041 tcaggagtga gcggaacatg ccgtcgggaa gcaccgtccc aatgcgcgag ccggtcacat
     2101 ccaaaaactt ctgcaggaga tttgacagcg gctgcccaac agcaaaaatg gtctggaaga
     2161 ccgcgatcac caccaccgca aagatcacgg gaccccaggt gcgattcaga aatacgttat
     2221 ccagtcggct cgtccaaatt ggggtggcgg ggcgacgata attcgcgttc tcccccacgc
     2281 gaacggccca ctcgcgcacg ctacgcgtac tgccgattac cggaagttcc atcggttcga
     2341 gggcaggtga tgctccggaa agaaaattgg tgaccgcttc aagaccggcg cccgtagtcg
     2401 cactgaccag ggcgaccggg gtccccagtt ggcgcgccag cgagagcacg tccacgccac
     2461 cgtcctgctt atgcaactcg tccgccatgt tgagcagcac cagcgtcggc aggccaagcg
     2521 cgattacacg ccctgccagc accaggtggc tatgcaggtt ggtggagttc aggaccacga
     2581 ttactgcatc aggacgattc atgccgggca tattgccctg caacacggcg atcgcgactt
     2641 tttcgtcttc cgatttagca gtgagactgt agatgccggg cagatcgatc agcgcgactt
     2701 cttcgcccgc gccgtcgcga atatagccgg tatggtgctc gacggtgacg ccagggaaat
     2761 ttccgacttt ctggcgcagt ccggtgagac gattgaagag tgtggtcttt ccggaattag
     2821 gcggacctac aacggcaacg gttcgatacg aagtgtcagg tgcttcgtcc tgggggagcg
     2881 ccacgggatg gcacgtcatc gctgaggctc ccttgggcta atcaccgaag gcaggggcgc
     2941 cgaccaggga cacgtgaatg tgccgtgaaa cctcacggcg catggccact tccgtccctt
     3001 cgacccgata gacccgggga tcgcctcccg gaccgctgcg ttcgacggtg acttccagac
     3061 ctggcacaaa acccaggttc atgagatgtt cagcgatggg gtgatcgagc tcaaactctc
     3121 taaggatggc gcgctcaccg acgcgaaggt cggccagtgc gccagactta tgatgaccgc
     3181 gttgcttccg tccgaatatg aagttgaaaa tcattttcaa ttccatttct attctatccc
     3241 tttcccggca gagggcaacg acgaaagtca cactcaattg tgataagtgc acaattctgt
     3301 tgcaaatctg ttgcaactcg ctttggcgca cttaactcgt tgacggtacg tcacttccgc
     3361 agtatttatg gaatccactt tgcggctcaa gatgcaacga aattgcgggc ttcccaactc
     3421 cggagacgtc ggcaccctgc tgtggcgtcg ctcaacaaac tgaacttcct tgcatgtggc
     3481 acactgaagc agcgcgtcca gatcgccgga tgtgcggacc atccggtcgg gagtttgtcg
     3541 catgttggga ctaagcgaaa tcgcaagccg tattgtgcaa tccaccattc gcaccatgtc
     3601 ggtggagtgc gagaaagtgg gcggcgtcaa cctggcgcaa ggtgtgtgcg ataccgaggt
     3661 tcccgcgatt gtccggcaag ctgccgaaaa tgcgattgag gcgggataca accagtatac
     3721 tcgcgccgac ggcatcgctt ccctgcgcga ggcgatcgca aagaagcttc agcggcagaa
     3781 cggaatagtc gccaatccca attcagaaat tctcgttacc tcgggagtaa ctggcggctt
     3841 cgattcggcg tgtcgcgcac tcttgaatcc cggcgatgag gtcgtgttgt tcgagccgta
     3901 ctacggttat cactatgcaa cgctggtcgg ccaatattgc aggcccgtgc tggtgtcgct
     3961 cgatccgccc gactacccgc tggtggcgga caggctggct gcggcaatca ccgcgaagac
     4021 ccgcggcatt gtggtgaaca cgcccgccaa cccgtcgggc aaagtatttt cccgcgtgga
     4081 actcgagcag atcgccgccg tcgcgcgcca gcacgacctg ttccttttca ccgacgaaat
     4141 ctacgagcat ttcgtgtacg acggcgagcg ccacatcagt cctgcgtcgc tacctggaat
     4201 cgccgaccgg gccatcacga tttccggatt ctcaaaagtg ttcagcatca ccggatggcg
     4261 tgtcggatac ctgcatgcat ccgcgcagtg gatccccgcc atcagctact tccatgacct
     4321 cacatatgtt tgtgctccgt cagcgtttca gcatggcgcg gctgcggggt tggagcagct
     4381 gcccgattcg ttctacgcca atctgattca ggactatcag ctcaagcgcg agatgatatg
     4441 cgctgccctg cgcgtggccg gtatggaacc cagtgttccc gccggagcgt actacgtgct
     4501 ggcagacgct tcggtggtgc ccggcgccga cagtgtggag aaggccatga acctgttgcg
     4561 gcttaccgga gtcgccagtg ttcctggaga cgcttttttc caaggcggtc gtggcgacca
     4621 ccttctgcgt ttctgttttg ccaagccgga taacgatctg gcgaatgcct gtgagcgcct
     4681 ggcgggattg ccccgcggcg cctgaatcca gcctcgcgaa gccgttgttg cacaaggaaa
     4741 acgcgcattg ttacagctcc acaatgtaga taacgggatc gaatacctgg cataaatagt
     4801 ttgaatttcc catctcacaa aaaagcctgt atttctaaat tcaccaccag aaacactcag
     4861 ccaacgcagc cggcgcgttg gtatttttcg ggttgtggga atgcagtctc aaatgcggtg
     4921 acgcaacggt cgcgcgttcg atatctcggc gcgtgatggt tcatgaatta tgcaaccggg
     4981 ggttcttcac tagttgtgtc ggacaacctt gccgtagccc aggactgcag cgaggctgaa
     5041 gttttcctgc aacttcgcga gtggccgaaa ccctgaaaca gccatgtcca cgccaacctt
     5101 cgatcccgcg ctgcgcagct tttacaaaga ggggtccgat cgcgtccgcc gggatttcga
     5161 ggccaatggt tcaggccgct cctgcgtaca agaccgcacc cagattgtcg acaaattgct
     5221 cctgcattta tgggagcagc agcacgcact gtgctccaat accggtttcg cgctggtagc
     5281 actgggcggc ttcggaagac gcgcgctgta tccgcactcc gacatcgacc tgctctttct
     5341 ctgtgagaat gaaactctgc gaacacgggc aaaggacccg atacgcgcca tctgccagga
     5401 actttgggac accggcctgc gggtcagccc gactactcgc accatcgacg actgcggacg
     5461 cttcgaccag gacaacgtcg agttcacgat ttcactgctc gactgccgtt tcctcattgg
     5521 ggacgaaaaa ctttttgcgc gcctgcacga caagaccctg ccgcagttgg tggcgcgtga
     5581 gtcgggcatt ctcatccagc gcctcactga agtcacgaag acccggcatg tcaaattcgg
     5641 caagaccatt ttccatctcg agcccaacct gaaagatggg ccgggcggtc tccgcgactt
     5701 ccacgtcacg cgttggatcg ccatgatcgc ggcgctcgaa gccaatcgca aatggccgga
     5761 atcgttgcac ctgcagcaga actccgacga cgacgatgtg ctcgaggcca tggagttcct
     5821 ctcgtcggcg cgctgctatc tgcactatcg caacaaccgt gacgagaata tcgtcacctg
     5881 ggaggcccag gaagacttgg cgagtcgcgg catcggaagc ggtatgggtc cgttgacgtc
     5941 ggcagagtgg atgcgcctgt acttccgcaa cgccaaagcg acctaccgca acgccacgca
     6001 gttgttggag caaacttccg ccaaccgttc gtcgctagtg cgttccttcc ggcgttggcg
     6061 gtcgcgggct tcgaacgatg aattttccat cgtggaccgg cggctctaca ttcagcaagc
     6121 ggctggcgcg cgcgacgcgg aggttgtgct gcgccttttt gttttcatgg cgcggcacgg
     6181 cgtgagtctt gccatggaaa ccgagcgtcg cctcaacaac gcccgccgtg ctctgcgcga
     6241 caccatgccg caggacgcta ggctgtgggc gtgtctgcgc gagttgctgg tgcagccgca
     6301 tgctgccgaa gcgttgcgct ccatgcattc tctccgcctg ctggcaatgg cgctgcctga
     6361 atttgagacc atcgactcac tggtgctgcg ggacctctat caccgttaca ccgtcgacga
     6421 acacacgttc ctggcaattg acgtgctgca tcgcctgcgg gacaacgagg ttgagtggct
     6481 gcagcccttc gcagggctct tcaccgagtt ggaacgcccg gagctgttgt ttcttgcgtt
     6541 gctctttcac gacaccggca agggactggc aggcgacaac catattcacg gaagcctcca
     6601 actcgcgctg gcggctgtcg aacgtctcgg actaagcaaa gaggatgccc aaacggtcgg
     6661 ctttctgatt gccggccacc ttgaaatgtc ttcgacctta cgccgacgcg acatctacga
     6721 tccagagacg gtccgcgagc tagccggcaa aatggagacg ccggagcgtc taaaaatgct
     6781 gaccttgctg acgctggccg acatcagggc ggtgaatccg gacgctctca cgccatggaa
     6841 agcggaaaat ctctggcagc tctatatcgc caccgccaac tatttcacgc gcagcgtcga
     6901 tgaacagcgc tttcatgccg aggtgtgtac cgagcaagtc gagcgcatcg cggccctgct
     6961 tcccaagaac aggtcgcaac tgctgaaatt tctcgatggg ttgccgcagc gttacctgat
     7021 gtcgcacgcg cccgcgcaag tgatcgaaca ctttgaaatg gcgagccggc tgggaaccga
     7081 accggtgcaa ctcggactcc gtcgcacccg cgagcagcac gagcttacgg tggtgaccga
     7141 ggaccgggaa ggattgttcg ctacgctgac aggcatcctg tacggctggg gtatggacat
     7201 caccaaggcg agcgcttttt ccaaccgggc cggcgtgatc gttgatagct tctatttcaa
     7261 agaccgcttc cgcactctgg agctcaatcc atcggagcgg gagcggttca agaagtcagt
     7321 catcgacgta ctcagcggcg actcgccact cgacggcctg ctggagcccc ggctgcgcgc
     7381 cgacactaag gcgccaaaac tgaaaatcga gacccggctc cgctatgacg acgaatgctc
     7441 tcccggcagc acgctgcttg aggtggttac gcaggaccgc ccgggattgc tgtacaccat
     7501 cagctcaact ctggccgcag aaaattgcgg catcgaggtt gcgctcatcg ataccgaggg
     7561 cgagatggcg catgacgttt tctacctgac gtccgggggc atgaaattga cgcgcgatca
     7621 gcaacgggca gtcgagtggg cgctcacaac cgagcttggt gaatcgctgc cgtcgagctg
     7681 gtgatacttc gccggctgcc cgttctaact catcatcgcg acccgttctt cacttcaaca
     7741 ccatcgtcac tgtaacctcc gccctctttg caaagactaa gtatcagtgt tgaaatgcat
     7801 tcaggtagat ttctcccgca tcgctgccca tgaaatctag tctcgcgagc acgcatccag
     7861 caacccgata ccgtttatat cgtcagcaga aggcggccga tcgcctgtga cggccctcga
     7921 catacgccac gttcttgctg actggcagat cattgctgcc tggatcgttt tcctcgccag
     7981 ctattttgtc ttcgcgttcg gacgcctgcc gggcacgaag atcgatcgcc cagccatggc
     8041 cgtggtcggt gcggtcctga tgttcgtgtt ccgcatcctg acgccgcaaa cggcaatcgg
     8101 gagcgttgat ttcggcaccc ttgttctgct tttcgcgatg atgctgatcg tggccagcct
     8161 gcatctcgcc ggctttttcg actgggtcgg caaccgcgtc atactccatc tcgctccccg
     8221 ccagttactg cccggcgtga tcttcactag tggaattctt tcggcattcc tggtgaacga
     8281 cgtagtttgt ttgttcatgg ccccactcgt cctcggcatc tgcaagcgga tgggactgcg
     8341 tccccttccc tacctgctgg cgcttgctac cgcttccaac atcggtggcg tggcgaccat
     8401 taccggcaat ccgcagaata tgctgattgg caccgtgtcc ggcatcgcct atcgcgactt
     8461 tctcgcccac ctcggtcccg tcgccgtgat cggtttgttt cttgactggg caatactcca
     8521 ttgggcttat gtgcgaggca atatcgcagc tgtcgaccct cagccggaga tctcggaggc
     8581 aaacgttgtc agcgccccgg ccaatcgtcg catgctgtgg cccgtcatga taaccatcgc
     8641 ggtgctggca ggctttcttc tgggattcca tccgccactg gttgcagccg caggcggcgc
     8701 cctgctgttg gtgcggcgca ctcgcgagcc cagagatgtt tacggcgacg ttgactggtc
     8761 tctgctggtt ctttttctgg gattgtttct gattattggt ggagccgagc aggagggcgt
     8821 gaccagccaa ctgctgagag cagcagagcg cctgaatctt caccacagcc tggtgttcgt
     8881 atcgatggtg gcgctgctct ccaatctcgt cagtaacgtt ccagcggtaa tgcttctcaa
     8941 aggattgatc ccgcgttttc aagacccaca caccatgtgg ctgatgctgg ggatgtcgag
     9001 cacgctggct gggaacctga ccattaccgg atcggtggcc aatattatcg tggtcgaaaa
     9061 agcccgcacc gtaactcaca tcagcttcct cgattacatg aagacgggag ttccggtgac
     9121 catcgccacg ctggcggtcg gtctgttgtg tctcaatttc gtccgctatt gatgcttggc
     9181 tgcagtggtt accgcgtagc cgtggctgat atcggaaacg tgtctttggc gccgattgcg
     9241 ctgattgcgg tgaagcggag ttccgccgtc aggcccttgt caatcaagtg tgccgccact
     9301 ctcgcaccca cctcgtcgcg gtctgcatcc tcatgcagga acatcagcac agaaggaccg
     9361 gcgccgctga gcgcgactcc gtttacgcct tccgcacccg caagagcagt caaggctggc
     9421 agcaaggggc ataactgcgc gcgatagggg tggtgcactc gatcgctcag cgcttcgttc
     9481 agccactctc cacggccttg gctgaaagca agcaccagca gcatcgcact ctgtacgttg
     9541 tgaacgagat cgacgcgact gtagagacga ggcagaactc gccgcgcttc cgacgttgcc
     9601 aggggatttt ctgggatcgc gatcagcagc ggccaatcga ccgccaccgg gatcggaagc
     9661 acgcttatgg tcgggacgga agcgttgcgg actacggacg ctgtccgcgc caggacgact
     9721 ccaccgtgcc agcaggcggc aacgttgtcg ggatggcctt cacaggtcga agcttctgag
     9781 acgattcgtt ccccgtccca gtcaagggcg ccgaagtatg cagccatggc cacgctggcc
     9841 agcacggcgg cagctgacga accgcaaccc attcccagcg gaatgtcgtt gtcgagacga
     9901 atcgccagcg gtgttgcgtg cttcccttcg cgctccagca gccgggtgta ggtgtcaacg
     9961 atcagattgc cgggcatctt gccacactga tcggcgttgc ggccgtgagc atcgattgag
    10021 aattcggccg caaggatagc ttcgactcgc aacccaaaat ccaacgccag ggccgcagcg
    10081 tcaaaggccg gaccaagatt cgccgacgtc gcaggcagga acatgcggaa acgcagggca
    10141 gacgagtcgc ttcgctcagc catttcgctt ttcctcggcg acgagcgctc gcaaaactgc
    10201 gtccgcgtcg gcatccaaga cgatcggcgc ctttctttgt tgcgccgtca gcgcatgaaa
    10261 ttcgttatcg gccattcgcg ggaccgagag atcgccgcga tgaaacttaa tggtgtactc
    10321 cgggtctttc agcagatgcc ccgtcagcac tagcaccacc gagtcgctac gctgtacgaa
    10381 gccgctcttg accagcttct tgagccccgc cagcgtgaca gcggaggctg gctcgcatcc
    10441 cacaccatcg gcgccgattt cagctttggc caccgctatc tcggcttcgc taacctgctc
    10501 aacacatcct tcagtcgcct cgatgactct cagcgacttc atccaagatg cgggattgcc
    10561 aatgcgaatc gcggttgcca acgtgtccgc ttcgacggtc acaagttcct tgccgaactt
    10621 ctcatggacc gtacgataga gcggattcgc gccttccgcc tggatcacag atatcttcgg
    10681 cacggattga accacgccga gacgcttcat ctcgagaaac gctttcccga gcgcggaatt
    10741 atttcccagg ttgccacccg gaacgatgac gtggtcgggc acctgccatt ccagttgctc
    10801 gagcaattcg atggcggcag ttttttggcc ttccagccgg aacggattca cagagttcaa
    10861 caggtagata gggtagcggc gaatgacgtc gttcaagact cgcacgcatc catcgaaatc
    10921 ggtgcggagt tggcaggtca gcgcgccata gtccagcgac tgtgagagct ttccccaggc
    10981 aatcttgccg tctggaataa ctaccagact gcgcatccct ccgcgcgcgg cgtacgccgc
    11041 catggaggct gaggtgttcc cggtggaagc acaagccacc cagcgaaact tgttctggcg
    11101 tgcgaaggag atggcgacgg tcatgcccgt gtctttgaac gagcccgtgg gattcatccc
    11161 ctgatgcttc gccctgaggc gttcaatccc ggcggagcgg gcacagcccg gcaagtcgta
    11221 aaccggggta ttcccttccc gtagcgtaat cgccgacgac cagtcgtcga gttcgggaag
    11281 cacgtcgcgg aaccgccaaa ctccactggt gtccagaggc aggttcgatg tcctgcggtc
    11341 gcgccacaag gacttcaatt ttgccggatc gagcgcagac ccgtctgacc agttgatgaa
    11401 ctccacttcc agcaggtcgc cgcagttacc gcaacggaaa tcgaccgcca gcgcagctgt
    11461 cgcctcgccg cacgagatgc agcggacacg gtagttcttg gcggcagtaa cactgtcagc
    11521 gtgcaggaat cccattccgc aatcccaggt tttgaatacg tagctgtcat tctaagttac
    11581 aacgattgaa cattgctatc agaagtcgcg caccaagcag ccacaatcac tgtggtggtt
    11641 ttcgatgctc ggttatggca caaatgcata gggaggagtt ggtcctcctc cctatcagac
    11701 tactttgggt gcggctggtt ctgcttcgtc gttactcgac gtaaggcttc cagactccgt
    11761 ccttggtttc ggccagtacc acgcttgcct tggtgggtgc gccgttgtag aagtcaacgc
    11821 cgtagtcctt gatcagtgtc ccgcggtccc aggtgttgaa cgtgtgcacc atgtcgctcg
    11881 aggcatcgaa ctccttgccc aaacggttcg cgtcccactt ccaggtgtag tccacacgcg
    11941 ccagatgcgg cttgatcatg gtgatcttgt cgatcgagac gatcttgcgc tccgcgagcg
    12001 gcaccacata gttggtattg ccgtcggagt tcttgccctt atcgacgccc ttgatttcgc
    12061 tcagcagctt ctcgcctgcc ggcgtcatcg tggcataaat ggaattccag ctctttggtt
    12121 tgatggtcag gatgccggct ttggccagca acttgtagtg cggatccatc ggcttctcgt
    12181 tcacgctcga cacgatggtt ccaacgctga aatggatgtt gccgccggcc tgggacttca
    12241 gtatctcgtt aatcgagttg gtggcaacag gcgccgtcag gacttcacgg gacgtcttca
    12301 caaagtagaa aatcgttccg cccacggcca aaaccaaaac caggaccatc acaagtggac
    12361 cgaagttgga ggacttgctc tccatttcaa tttcggcggc gaatagctcg cgctctttat
    12421 aagtaggagc ttcgggatta gtagttttat ctttaagttc gggatcgacg acgattggca
    12481 ttgcctcaca cctccgcttg ggacgtgctg ccggccgctg aagccgaggc tcattgcaac
    12541 ccaacttgcc cagttggctt cgcttgcagg ttggaaaacg tcccctgcct ggctcggagc
    12601 agccataaac aattccagta tgaaccttgt gcacagggac gcagtgagca tcatcacagc
    12661 ccagcgtgag ggatgaactt cgtggccgag tcggcatgtt tgcggcctgg tggtctcaag
    12721 gagggccgct ttcagctact gcaggatgtg gcaggagtag gttttcacca ccagccagag
    12781 ttgtttcccc acttccaagt tcagagcctg gcatgcgcct ggagtcaggt gcaccacgaa
    12841 cctggtgccg caatcgatct cggcaattac tgtgacgtcc tgctgtcgca gcaagacgat
    12901 aaacccagca atcacatttc tcgcactcaa gccttgcggc agtgaggttg ccaccaggat
    12961 gtcgcctgcc cgaatcccta ctcggaccgg tctcgcggta tccacccgcg aaaggggcac
    13021 ttccagcgaa acctctgtgc cggcgatgcg gcacgtcatc gtgccttgct ctgcatgaga
    13081 cgcaatcacg gagcattcca cgatgttttc gaatcctgcc agctgtgcca ccgactcaaa
    13141 ctgcggacga ctgagcacgt cgtgcggagt cccatcggcg atcaacttcc cggcctccag
    13201 cacgagtacc cggttgccca gcgcgtagac ttcttcgcgt tggtgtgtga catagagaat
    13261 cggaacagca tggcggtcgt tccacagtcg caaatcgtcg aggatgcgag tcttcgtacc
    13321 tgcgtcaagc gcggttagcg gctcatccag caacagggcg cgaggtcgcg tcaccagggc
    13381 acgcgccagg gcaacccgct gcctctctcc tccggaaatt tcaaacggtc gccgatcaaa
    13441 caaaccggat atcccaaacg actctgcgat ttcgctgaca cgattctcgc gttcatgccg
    13501 gtcgagcata gacaacccgt atgcaatgtt ttgccggacc gtcatgtggg gaaacagcgc
    13561 cagcgtctga aataggtatc caatattgcg gctgcgcgcg gggagttcaa ttcccttctc
    13621 tgagtcgaag agaactatgt cgcccactgc aatgtgacca gaatcaggct cctgcagccc
    13681 ggcaatgcag tccagcaccg tcgtcttgcc tgcgcccgag gcgccaaaca gaatcgtgaa
    13741 gccgctggcg atcatgaatc ggacgtcgag ttgaagcgag gtatcttccc tgccctctcc
    13801 cccgaggcgt ttgtgaagac tgacgctgag ctcacgcgcg ggaatctgga tttcggcgct
    13861 gctcactgcc acccccacaa tacccacttg ctcttgcgac gcaatccata aagcaatgtc
    13921 agcaaggcga agcagaggag cagcaacacg agcgccgtac gatttgcgac ggcgtagttc
    13981 agagactgaa cctggtcgta aatcaatatc gaaactgtct gcgtcgcccc cggaatgttg
    14041 ccgccaatca tcagcaccac accaaattca cccagcgtat gcgcaaagct caacacgaac
    14101 ccggttagca cgccggggaa tcccaggggc aacacgattc tccaaaacgt gcgccagcgc
    14161 gacgcgccta gcatggccga tgcctcgatc agcttgcgat cgacggatgc gaacgctgcg
    14221 gcaatcggtt gcaccgcgaa tggcaagctg tagagcaccg agcccacaac cagtccgcga
    14281 aaagtgaaag cgagactgct ccctgtgatg gagtgatacc agcgtccgaa cggactcagc
    14341 gggccgattg caaccagcac ataaaagcca agcaccgtcg gcggcaatac gatgggaagt
    14401 gaaacgatgg cttcgaccac gaatttcccg cgccagcgcg acgacgccag ccaataccct
    14461 agcggcaacc cgatcgccat gagtatcaag gagaccagaa ttgccagccg cagcgtaagg
    14521 gcgagtgcct ggaggatcat ggttttgccg gaggcgtcag accgtacttt cgaagtgtag
    14581 ccgcgccctc gggagatagc acgaagtcca taaacgcctg cgccgctttc ttgtgtttgg
    14641 aagcggaaac aatcaccgcg gcctgcttca actccggata cgagtcggat ggtagcaccc
    14701 agaatcggcc cgccgattcc atcgccggag acttggcgag agacatcgcg atcagtcccg
    14761 cctgtgcgtt gccggactgc acaaattggg cagcctggct gacattctcg ccaaatacta
    14821 gcttaccggc gagtttgtct atcagaccgt agtgctcgat cgctgtcatc gcggcccgtc
    14881 cataaggcgc atgctccgga ttagcaatgg caatgcgtgt gacgcctggt tggtttagca
    14941 attccatctg caacttctgc ggatcgaagc tcgagttgtt gggcacccac agcacgagat
    15001 gtcccagtgc atagatgcgc agcgatgagg catccgcaac tccggtattg accaacttct
    15061 tcgagtagtc ttcgtccgca gaaaaaaaca gatcgaacgg agcgccgctt tgtatctggg
    15121 aaaacaggtt cccggatgct ccaaatgaaa gcgttacttt ggttcccgtc gcagtctcaa
    15181 accggcccgc caactcattc atcgcgtagt taagatccgc tgcagcggcg acggttatcg
    15241 gctcggcagt cgcggtgcga aaagcaaaag caacgaatat caggagcatc gagatctctt
    15301 gcatcggtcg cgcttattag tccttccgta agacgggatt ccctcatcaa aggcctccgc
    15361 tctagatggc agcagaggcc gcggcaaatt gctctcagct taacgtatct cttcgctctt
    15421 gattggcgtg gatattaccg gcatctttgg gattcgcgct tgaccagtag aaccattcct
    15481 gtcagagtaa ccgaggctat atccggtggg gatgacgcta gccttctggc cgctttacgc
    15541 cgattgagtt tgatcgcgac aaactgatga tacgtatcga ggttacactc aacgccgacc
    15601 cgactgctgt cagtcctgtg gtcgagggca tcatggagat ggcccgccgc atggattgcg
    15661 ccgccggcaa ggaatttgag atcgaaaccg cgctccgcga ggcgctggcc aatgccatcc
    15721 tccatggctg caagaacgat cccacaaaaa aggttcagtg ttgtgtctct tgcgacgagg
    15781 aacacggact cctcatcgtg gtaaaagacc ccggtaccgg cttcgatccc gtgcaggtac
    15841 ccgaccccct ggtgggcgaa aacgtctact ccaaccacgg ccgcggaatt ttcctgatca
    15901 accagttgat ggatgaggtc cgattcgagc gaaacggcac cgaaatccac atgcgcaagt
    15961 attagcccac catcctggct tcctgtacta acgacgtagg cgatttgtgc ctatccgccg
    16021 gggcgcgaca ggacgtgcgt aaagcgctgc ttcagactca ggaatggagt ctccagccag
    16081 cgataagaca ccgccgccat ggcgatggta gctgccagcg caactcccac ccgcaacgaa
    16141 taccggaaga ggctagcggt ctgatctggc accgtgtgat cggaaatcag cagacccagc
    16201 acgtggaaga cgtagagccc ataggaaatg cgacccagat aaatcagcgc tcggttaccg
    16261 ccgctcgtgc gagatctcag caccgatagc aacagcagcg cgcccccgat ggctaccgat
    16321 ggatatccca gtacgattcg cgccgttgtc aaaggatcat tcttgatccc gaaatacagg
    16381 ctgccgaacg ccagtgctgt aattcctgcc agcatcatcg cgacgcgagc tagtctcgac
    16441 aagcgcggaa tttcgccttt cagcaacacc gcaatcagga ttcccgccgc aatcggatcg
    16501 agttgtacga aggtgttgca ccacacgtca ccttcgagcg catgatccat gccgaggtaa
    16561 atgcgggcaa cggtagccgc cgctagcagc cctccggcga aaagcagcat cccggtttca
    16621 ctcaccttgc gaaccaccgc cggccacgta atgtaaaact gctcctcgat ggagacgctc
    16681 cacagcggaa agatgactga cgacggaaat ccatgaaaga cgatgaacca gttgcccgcc
    16741 agaaatgaaa accaaacgcc agcgcgcaac gtaacgtgct gaccgggtac gatccactgc
    16801 aacgccactg ccagagcgag gaatgcaaag tagagcggcc agattcgcag aatgcgtcga
    16861 atgtagaacg agcgtatgtc gagagtgccg atcaactgct tctcgcgcag tagcaattcg
    16921 gtgatcaggt atgagctgag cacgaagaac aggctgacgc cgaacgcacc ggtcgcacct
    16981 actccagcca ctacccgcgc caggaatact ggtgacccca ggttcgtcca gaatgtagga
    17041 tcgtggggca acgcgtgatg cagaaatacc atcaggaagg cgaagaaacg gagttcatcc
    17101 agttccggat ggtaaaagcg cgcagttgag gtgcgcgcgc tccccgggcc ggcaagttcc
    17161 gaggtcgttg tagtaacaga agccaatagc aaattctaaa tgacgcgcgg acaaatcggt
    17221 gagtgtggac acatgcgctt g
//
LOCUS       DASRKK010000640         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_158299, whole genome shotgun sequence.
ACCESSION   DASRKK010000640 DASRKK010000000
VERSION     DASRKK010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158299"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFC15_15680"
     CDS             complement(<1..322)
                     /locus_tag="VFC15_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZM11647.1"
                     /translation="MQIVKVSLGNRSYDIKIGTGLLARVGRECSRLALNRGCTIISDR
                     NVAPRYAKAVQDALTKAGFAAGLITIPAGETAKSLKTLAACYGLLATQRLERKSFIVA
                     LGGGV"
     gene            731..2242
                     /locus_tag="VFC15_15685"
     CDS             731..2242
                     /locus_tag="VFC15_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11648.1"
                     /translation="MGILHSFDRLRLQGSLRYLYCTEIFEEYLSKAKVLCKDFKQHVK
                     GVTAQICRKAEELAQSLGRPYTYFASSRLSKEEEAQKIVEQEGLKEGLVAVFSCVEPC
                     RTYKMRGNYQTKMLEPRLEWGKCLHLYFYVQHPRFGLLHLRLQTWYPFLIHICLNGHE
                     WLARQMEAKGLSYRKSDNKFTWIEDFAVAQALADEQLRTDWVALCEGLRRTYHPLHEQ
                     IGRPLNGLSYYWTAPQTEFASDVIFRDQASLDRLFPRLILHGILNLGCEQVMRFLGKQ
                     LNGRFGGEVVTDLRRRSEGVRLKHWVNDNSIKLYNHLNVLRPETTIHDAEDLKVYRTP
                     ESRPDGRKAWYPLRRGVADLYRRAQISRTANERYLAALAATSVTTPLAQEAVTICQPV
                     RRQERRYRALNPFEAADAQLLAAVNRGEWALKGFRNREIRALLFDEAKDKKQERSQAA
                     KVSRRLALLHAHGLIAKVSRTYRWQVTAKGRRILTALSAARHADTDKLMSLAV"
BASE COUNT          510 a          686 c          711 g          468 t
ORIGIN      
        1 ccacgccgcc gccgagtgcg acgatgaatg atttacgttc caatcgctgc gtcgccagga
       61 gcccgtagca cgccgcgagc gtcttcaagc tcttggcggt ttcgccggct gggatggtga
      121 tcagcccggc ggcaaaaccc gctttcgtca aggcgtcttg cacggctttg gcgtaccggg
      181 gtgccacgtt gcggtcggaa ataatggtgc aaccacggtt gagcgcgagc cgggagcatt
      241 cgcggccgac gcgggcgagg agacccgttc cgatcttaat gtcatagctg cggttgccga
      301 gtgatacctt aacgatttgc acgtcccaag gatagggttt aggcccgcgg tgtcaaaggt
      361 ttgccgcgcc gcgtaactgg gaggcggggc attggtaccg ccatgccgga tgaccgcccg
      421 ggagcgcagc ctttagccgt aaccatgcgg tggacggaag cccagtaaaa ccgcagagac
      481 gcgcagagca cagcgcggca aagccgcaac cgaagttgtg gctgcgcgtt ggtatgataa
      541 gcataacgaa gatggcggat ggcggatgga gcatggcggt cgctgaacct cgaactttga
      601 accttgaacc cggaacctcg aactgacagg acgtggttcg tggagtagaa gggttttatg
      661 caaaagaaaa catctgctcc aaccaaccac gccctcaacc ccgtttatag agaaacacca
      721 gcaggatgtc atggggattc tgcactcctt cgaccgcctc cgcctgcaag gttcgctccg
      781 ctatctttat tgtaccgaga tctttgagga atacctgagc aaggctaaag ttctgtgcaa
      841 ggacttcaag cagcacgtca agggagtgac ggcccaaatc tgtcggaagg ctgaagaact
      901 ggctcaatcc ctgggacggc cctacaccta ttttgccagc agccgcctct ccaaagaaga
      961 ggaggcgcag aaaattgttg agcaggaggg tcttaaggag ggcctggtgg cggtctttag
     1021 ctgtgtggag ccttgccgga cttataaaat gagaggcaac taccaaacca aaatgctgga
     1081 gccgcgcctg gaatggggga agtgcctgca cctctacttt tatgttcagc acccgcgctt
     1141 tggtttgctg catttgcggc tgcaaacctg gtatcccttc ctcatccata tctgcctcaa
     1201 tgggcatgaa tggctggccc gccagatgga ggcgaagggc ttgagctacc gcaagtcgga
     1261 taacaagttt acctggattg aggattttgc agtggcccag gctctggccg acgagcaatt
     1321 gcgcaccgat tgggtggctt tgtgtgaagg gttgcggcgg acttaccacc cgctgcacga
     1381 gcaaatcggt cggcctttga acgggctttc ctattactgg acggcgcccc agaccgagtt
     1441 tgccagcgac gtgatctttc gcgaccaagc cagcctggat cggttgttcc cgcggctcat
     1501 tctgcacggg atcctcaacc tgggttgtga gcaggtaatg cgttttctgg gcaagcaact
     1561 caatgggcgg tttggcggcg aggtggtgac ggatttgcgg cgccgttccg aaggggtgcg
     1621 cctcaagcat tgggtcaacg acaactcgat caagctttac aaccatctga acgtgctgcg
     1681 gcctgagacc acgattcatg acgccgaaga cctgaaggtc tatcgcacgc cggaaagccg
     1741 ccccgacggc cgcaaagcct ggtatccttt gcgccgcggg gtggcggatc tttaccggcg
     1801 cgcccagatt tcccgaaccg ccaatgagcg ttacctggcg gctctggcgg cgacctcggt
     1861 taccacgccg ctggcccaag aggccgtcac tatctgccaa ccggtgcgtc ggcaagaacg
     1921 ccgataccga gcgctcaatc ctttcgaagc cgccgacgct caactgctgg cagccgtcaa
     1981 ccggggagag tgggcgctca agggcttccg caaccgagag atccgggcat tgcttttcga
     2041 tgaagccaaa gacaaaaagc aggaacgctc ccaagcggcc aaagtctcgc gacgattggc
     2101 gctgctccac gcccatggtc tgatcgccaa agtctctcga acctaccgct ggcaagtgac
     2161 tgccaagggc cgccgtattc tcacggcctt gtcggcagcc cgccacgccg acactgacaa
     2221 actcatgagt ctggcagtat gaaatattca tcgcagcctg cgaacaattt ggtctatagc
     2281 agtgcagagg atttcctcgt tgtccacgcc aaccacagcc acttgctccg gcaccaatgc
     2341 gcccaggcgg gcgcaggcgt cgagcacatg cagac
//
LOCUS       DASRKK010000641         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1038765, whole genome shotgun sequence.
ACCESSION   DASRKK010000641 DASRKK010000000
VERSION     DASRKK010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038765"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..480)
                     /locus_tag="VFC15_15690"
     CDS             complement(19..480)
                     /locus_tag="VFC15_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM11649.1"
                     /translation="MRFRFIEDRRADYPVTILCDVLGVSPAGYYAWRSRPESERSAAN
                     RDLVEDIRRVHRDTSGRYGSPRIHAELKARGRGASRGRIERLMRHHGIRAIMARPRRV
                     RTTDSRHDFPIAPNLLDRNFTAAAPNRIWLADITYGAPRPVISGKRMFGMH"
     gene            complement(477..797)
                     /locus_tag="VFC15_15695"
     CDS             complement(477..797)
                     /locus_tag="VFC15_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11650.1"
                     /translation="METRQRRSFTDDYKRQAVDLVVSSGRSIGSVAKELGLRDSVLRR
                     WVELRGAGREPTAASRRPTTQATLPSADHAAEIARLQRENERLRMERDILKKSIAIFA
                     GTRT"
     gene            complement(1039..2757)
                     /locus_tag="VFC15_15700"
     CDS             complement(1039..2757)
                     /locus_tag="VFC15_15700"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZM11651.1"
                     /translation="MQQLLEHPTIAELTTVTTSLSGYQLLADPLLNKGTAFTEAERGM
                     FDLHGLLPPNIGVLDEQISRRLHALRNFKTDLERYSFLRGLHDANETLFFALIVKNIE
                     ELLPIVYTPTIGAGCQQFSRLFRKPRGLFLSLPHKTKLKTILDNPHFDRVEAIVVTDG
                     ERILGLGDQGAGGMGIPLGKLALYTACGGIHPATTLPIMLDVGTDNPECLADPLYVGW
                     RHERVRGAEYDDFVETFVSAVAERWPHVLLQWEDFAKGNATRLLNRYRDRLCTFNDDI
                     QGTAAVAIGTLLSAINVTKVPLTEQRVVVFGAGSAGCGIATLLLKAMEDAGLQQRDAA
                     EHIFMVDRDGLLVEGMGDIASYQKPFVQNKSVAGAWKLDHPDKVTLLDVVQNARPSVL
                     IGVSGQTGAFSEPVVRAMAEHNERPIIFPLSNPTSRAEATPEDLENWTDGRAVIGTGS
                     PFPPLTRGGTNFRVDLTNNSYIFPGVGLGVIAVKARRVTDSMFMAAAKALAAVSPARA
                     NPHGNLLPPVTSLRDVSAAVATAVAQQAHNEDLTKGINADEIEGLIRAHIWAPRYLPY
                     RRVNKP"
     gene            complement(2975..4282)
                     /locus_tag="VFC15_15705"
     CDS             complement(2975..4282)
                     /locus_tag="VFC15_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM11652.1"
                     /translation="MSTRDILGGRLLCAVKEAEMPDPPSGWSSTQRKVVLACYLGWTL
                     DAFDFFLLVFVLKDIAAEFHTEIGDVTIAILLTLAMRPLGAILFGRAADRYGRRPTLM
                     VNVVLYSLLEFASGFAPSLTVLILLRALYGIAMGGEWGVGASLAMETIPSRSRGFVSG
                     LLQAGYPSGYLIASVVYATLFPLIGWRGMFMIGVLPALLVLYIRRAVPESPSWQRTAS
                     SEGGTLSVLRSHWKLGLYAVLLMTAFNFYSHGTQDLYPTFLQVQHHLSPTVVGTIAVI
                     YNVGAILGGICFGMLSQRIGRRRAIVLAAVISLPIVPLWAFSTTPIFLAIGAFLIQFM
                     VQGAWGVIPVHLNELSPEGARGTFPGFVYQLGNLLASGNATIQAAIAVHYGNDYAFAL
                     MLVAVTVAIIIAAMTWFGVEAKDVTFGRGRLRAVDPPPEKYAA"
     gene            4657..>4859
                     /locus_tag="VFC15_15710"
     CDS             4657..>4859
                     /locus_tag="VFC15_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZM11653.1"
                     /translation="MNSTRAGAYTFIDHTFDVVVVGAGGAGLRATQGTVEAGLTTACI
                     TKVFPTRSHTVAAQGGIAASLGNL"
BASE COUNT         1077 a         1433 c         1414 g          935 t
ORIGIN      
        1 cgtcacattc ctcctttctc aatgcattcc aaacatacgc ttccccgata tcacggggcg
       61 aggcgcaccg taggtgatgt cggcgagcca gatccggttc ggcgcggcgg cggtgaagtt
      121 gcggtcgagc aggttcggtg cgatcgggaa gtcgtggcga ctatcggtgg ttcgcacccg
      181 gcgcggccgc gccatgatgg cgcgaatgcc gtgatggcgc atcaatcgct cgatgcgacc
      241 acggctcgcc ccgcggcccc gagccttcag ttcggcatgg atgcgcgggc tgccatagcg
      301 tccgctggtg tcgcgatgga cccgtctgat gtcctcgacg aggtcacgat tggcagccga
      361 tcgctcgctc tccggacgcg agcgccaggc gtaatagccg gccggcgaga ccccgagcac
      421 gtcgcacagg atcgtcaccg ggtaatcagc gcggcgatct tcgatgaagc ggaatctcat
      481 gtccgtgttc cagcaaagat cgcgatcgac ttttttaaaa tgtcgcgctc catgcgcagc
      541 cgctcgttct cccgctgcag acgagcgatc tcagctgcgt ggtccgccga cggcagcgtc
      601 gcctgcgttg tggggcgccg cgacgccgcc gtcggctccc gcccggcccc acgtagttcc
      661 acccagcgcc gcagcacgga atcgcgcagg ccaagttcct tggccaccga cccgatcgag
      721 cggccgctcg aaaccaccag atcaaccgct tgccgcttat agtcgtccgt aaatgatcgt
      781 cgttggcgtg tttccatctg acacctcttg gctctcagag cctactacag gtgtccatca
      841 attcggagga ggttcatatt gtcagcagtg cgacctgaag gtcttatacg ttacccctta
      901 acctgcgtca cgaattgccc tcagcccacg tttttgcggg gtctgacgat cacccaacag
      961 cagactatcg aaaagagcat ttgtacggga gttgtcgcaa catcgagaga cggcaaaatc
     1021 caatattcag acaatcgctt acggcttgtt gacccgtcga taaggcaaat aacgcggggc
     1081 ccagatgtgg gcgcggatca aaccttcgat ctcgtcggca ttgatgccct tagtgaggtc
     1141 ctcattgtgg gcctgctgag cgaccgccgt cgcgactgcg gccgatacat cccggagcga
     1201 cgtcactggt ggcagcaagt tgccatgggg attcgctcgg gccggagaca cggcagccag
     1261 tgctttggct gctgccatga acatcgagtc cgtgacacgc cgggccttga ctgcaattac
     1321 gcccaaaccc accccgggaa atatgtagga attgttggtc agatcgaccc tgaagttggt
     1381 tcccccgcgc gtgagcggcg gaaacggact gccggtgcca atgaccgccc gaccatcagt
     1441 ccaattctcc aaatcctctg gggtcgcttc agcccgggag gttgggttcg acagtgggaa
     1501 gatgatcggc cgttcattgt gttccgccat ggcgcgcacc actggttcag agaaggcgcc
     1561 agtctggccc gagactccga tcagcacgct gggtcttgcg ttctgcacca catccaggag
     1621 cgtgaccttg tccgggtgat ctagtttcca agctccggcg accgatttgt tctgcacaaa
     1681 aggtttctga tacgaggcga tgtcacccat accctcaacg aggagcccgt cgcgatccac
     1741 catgaaaatg tgctccgcgg catcgcgctg ctggagtccg gcatcttcca tagccttcaa
     1801 gagaagggtg gcgatgccgc agccggccga gcctgcgccg aacactacga cacgctgctc
     1861 cgtgagcggt accttggtga cattgattgc tgacaagagt gtgccaatcg ccactgccgc
     1921 cgtcccctgt atatcgtcat tgaaagtgca aaggcggtcg cggtagcgat tgagcagacg
     1981 agtcgcattg cctttggcga agtcctccca ttgcaacaga acgtgcggcc aacgttcggc
     2041 aacagcgctc acgaacgtct cgacgaagtc gtcgtattcg gcgccgcgca cacgctcgtg
     2101 gcgccagccg acatagagcg gatcggcgag gcattcggga ttgtcggtgc caacatcaag
     2161 cataattggc agcgtcgtcg ctggatgaat tcctccgcag gcggtgtaga gcgcgagctt
     2221 gcccagcggg atacccatgc cgccggcgcc ctggtcgcca agtccaagga tacgttcgcc
     2281 gtccgtcacg acgatcgcct cgacccggtc gaaatgcggg ttgtccaaaa ttgtcttgag
     2341 cttcgttttg tgcggaaggc tcagaaataa accgcgcggt tttcggaata ggcggctgaa
     2401 ttgctggcat ccggcgccga ttgttggtgt gtagacaatc ggcaacagtt cctcaatatt
     2461 cttcactatg agcgcaaaga acagtgtctc gttggcatcg tgcagccctc gcaggaatga
     2521 atagcgctca aggtccgtct tgaagttgcg aagcgcatgg agacgacgcg agatttgctc
     2581 gtcgagcacg ccgatgtttg gcggcaacag accatggaga tcaaacatgc cccgctccgc
     2641 ctcggtaaag gctgtaccct tgttcaggag gggatcagcg agaagttggt aaccggagag
     2701 cgacgtcgta acagtcgtga gctcggcaat agtggggtgt tctaacaatt gctgcaactg
     2761 ggaaccttcc tcacaaatga gttagacgac atagacgcgt gccatgacca gagaccacca
     2821 cgaacgagaa agagacgctg cagcggcggc tgcgcccttc cgactttgtc aggtgccccc
     2881 aatcagagat caccaagtgc gccggctcgt caaagaaaca atccgcgcgc attagcatgg
     2941 tccgacctga catcggataa gtcgcccagc agtatcaagc cgcgtatttt tcgggaggcg
     3001 gatcgacggc ccggagcctg ccccggccga aggttacatc cttggcctcg acgccgaacc
     3061 aggtcatagc cgcgatgatg atcgcgacag tcaccgcgac gagcatcagc gcgaatgcgt
     3121 aatcgttgcc gtaatgaacc gcgatcgccg cctgaatcgt cgcgtttccg gaggcgagca
     3181 gattaccgag ctgatagacg aaaccgggga acgtgccccg cgcgccctct ggagagagct
     3241 cgttgaggtg gaccggtatc accccccagg cgccctggac catgaattga atcaggaacg
     3301 cgccgatcgc cagaaatatt ggcgttgtcg aaaacgccca cagtggcacg atcggcagcg
     3361 agatgacggc ggcaaggaca atggcgcgcc gccgcccgat gcgctgcgac agcattccaa
     3421 aacaaatccc gccgaggatc gccccgacat tgtagattac cgcaatagta ccgacgaccg
     3481 tcggcgagag atggtgctgg acttgaagga atgttggata gaggtcctgc gtgccatggc
     3541 tgtagaagtt aaaagcggtc atcaacagca ccgcatagag gccgagcttc caatgcgaac
     3601 gcaacacgga gagcgttccg ccttcggacg acgcggtgcg ctgccagctt ggtgattccg
     3661 gaacggcgcg gcgaatataa agaaccaaaa gcgcagggag caccccaatc atgaacatgc
     3721 cgcgccaacc gatgagcgga aacagagttg catagacaac cgacgcgatg aggtaaccgc
     3781 tcggataacc cgcttgcaga agccccgaca caaagccgcg cgaccgggac ggaattgtct
     3841 ccatggcgag cgaagcgcct accccccact cacctcccat ggcaataccg tagagggcgc
     3901 gcaacaggat cagcacagtg agactcggcg cgaacccgga cgcgaattcg agaagcgaat
     3961 agagcacgac gttgaccatg agagtcggcc gacggccgta acgatcagcc gcgcgcccga
     4021 acaggatggc gccgagcggc cgcatagcca aagtaagcag gatggcaatg gtaacgtcac
     4081 cgatctcggt gtggaattca gccgcaatgt ctttgagcac aaagaccaga agaaagaaat
     4141 cgaacgcatc caacgtccaa ccaagatagc aagccaacac gacttttcgt tgcgtgctgg
     4201 accatcccga gggtggatca ggcatttccg cttctttcac ggcgcataac aaacgccctc
     4261 ccaaaatgtc gcgggtggac attgaatatg cccaactaag atttttgaca gactacacag
     4321 gggcgcttct gaagtctaag caaaattgct ctcttccgcg agacagggtg atctcagcgc
     4381 agaaagtacg caacctcctg aagccgtttt gtattcaagc tgtccaggac accacctagc
     4441 gcgttcagaa cgttgtctct tgttgcggcg cggccgttcg ccactcaatg tgagcaattc
     4501 tatacagtcg tcattgtggc attaagccta gcgttaagat cgggtctagc tatgcaatga
     4561 attcaatgaa acattgtgga tgaagcttcc tgtttgtctc tggcgtctgc gccgtattga
     4621 agatcggcat ccaatcctag cggatcaagg aacaagatga attcgacacg tgcaggcgca
     4681 tacacgttca tcgatcatac gtttgacgtc gtcgtcgtcg gtgccggcgg tgccggtttg
     4741 cgcgccactc agggcaccgt cgaggcaggc ttgacgacag cttgcattac aaaagtgttt
     4801 cctacacgca gtcacacggt agccgcccag ggcggcattg cagctagtct tggcaatct
//
LOCUS       DASRKK010000642         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1331978, whole genome shotgun sequence.
ACCESSION   DASRKK010000642 DASRKK010000000
VERSION     DASRKK010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331978"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFC15_15715"
     CDS             <1..588
                     /locus_tag="VFC15_15715"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZM11654.1"
                     /translation="LDLGSGRLGLVLADISGKGMSAALLMANLQANLRGQYALALEDV
                     PHLLRSVNRLFYKNTETSHYATMFFAVYDDETRRLRYVNCGHNPPMLVRASGAVEHLE
                     ATATVLGLFEEWECSVAELELAAGDVLVIYTDGVSEAADDKEEEFGETRLIEVARSHP
                     QHSADEILDAVLASVQGFSQGEQADDMTLIVARGR"
     gene            850..1476
                     /locus_tag="VFC15_15720"
     CDS             850..1476
                     /locus_tag="VFC15_15720"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZM11655.1"
                     /translation="MNRQDPEEERRRIAQIYAGMADGELEAIAVDSAGLTDVARQALT
                     DELARRRPGEGAAADTGVRPEHDSVELADVVSIRQFRDLPEALLAKGALDSAEIECFL
                     VDDNMIRMDWFWSNLIGGIKLCVKQEDADAALDLLEQSIPERFEVEGVGPYEQPRCPK
                     CQSLDIAFEDLNQPVAYVLAPIFLHRKRWVCHSCGHQWREPEDNQNAT"
     gene            complement(1921..>2365)
                     /locus_tag="VFC15_15725"
     CDS             complement(1921..>2365)
                     /locus_tag="VFC15_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11656.1"
                     /translation="RMIQLLLIVYAFFKLLDLVSNQALGYAFQRTSEAGYFWLEVALF
                     LAIPIFLLSFQSVRSNPERLYWTSAIVVAGFMVDRLNVSINSLQSAMGTHYVPKWTEL
                     ASSVLVIAAGVIAFRYAVIYLDILPKATVKPSTRWISDGGLPAQA"
BASE COUNT          518 a          648 c          719 g          480 t
ORIGIN      
        1 ctcgatctcg gctcgggccg cctcggcctg gtgctggccg acatctcggg gaagggaatg
       61 tcggccgcgc tgctgatggc caatcttcag gccaacctgc gcggccagta tgcgctggcc
      121 ctggaggacg tacctcacct gctgcgctcg gtgaatcgcc tgttctacaa gaacaccgag
      181 accagccatt acgcgacgat gttcttcgcg gtctacgacg atgagacgcg gcgcttgcgt
      241 tacgtcaatt gcggacacaa tcctccgatg ctggtgcggg ccagcggggc ggtcgagcac
      301 ctggaagcga cggcaacggt gttgggactg ttcgaggagt gggagtgttc ggtcgccgaa
      361 ctcgagctgg cagcgggaga tgtcctggtg atttacacgg acggcgtttc cgaagctgcg
      421 gacgacaagg aagaagagtt cggcgaaaca cgtttgatcg aagtcgcacg aagtcacccg
      481 cagcactcgg cggatgagat cctcgacgcg gtccttgcct cggtgcaggg attcagccaa
      541 ggcgagcagg cggacgacat gacgttgatc gtggcacggg ggcggtaggg gaaaactata
      601 ctagttgcta ttgccacaag ctacctcgta aatcgttttg tatcagggca cggcttcaga
      661 cgtgccgcaa ccgggcaaca atgacttggg cttcagccct gaaacccttt catgctgggg
      721 ctaaagccct gacctactgc gcgttcaacg gcacgactaa aagtcgtgcc ctgacacagc
      781 ccgacgccat ttaggagata cgttctggct tcaatgccgc ggcagggcgg ctgaaggagg
      841 ccgttaaaca tgaaccgtca agatcccgaa gaggaacggc ggcgaatcgc ccagatctac
      901 gccggaatgg cggatggcga gttggaggcc attgccgtgg attcggcggg gttgaccgat
      961 gtggcgcgcc aagctttaac ggatgaactt gctcgcaggc gtcccggaga aggtgctgcg
     1021 gcggacacag gcgttcgccc ggaacacgat tccgttgaat tagccgacgt ggttagcatt
     1081 cgccagttcc gagatctgcc tgaagcacta ctcgccaaag gcgcgttgga ctcggcggag
     1141 atcgaatgct tcctggtcga cgacaacatg attcggatgg actggttttg gtcgaatcta
     1201 atcggcggca taaagctctg cgttaagcag gaagacgctg acgcggcgct ggacctgttg
     1261 gaacaatcga ttcccgagag gttcgaggtg gagggtgtgg gaccgtatga gcagccacgc
     1321 tgtccgaaat gtcagtctct ggatatcgct tttgaagact tgaatcagcc cgtcgcgtat
     1381 gttctggcgc caatttttct tcaccgcaag aggtgggtat gccattcgtg cggacatcag
     1441 tggcgtgagc cggaagataa ccaaaacgcg acctaagaaa ggggcggaaa aaagcaagca
     1501 ccccgtgttt cgggcaccga aaagactgtg tcggaacctc tgccgtccct acgggactcg
     1561 ctcccatttc gcatcttacc cagcgctaaa gcgctgggct aagctctttc gccccatttg
     1621 gggctggttt tttgggtgct cgtttccaac gtcgcagatc gatacgagtt cgcgagacaa
     1681 tctctgaagg aaggctctgc cggcacccaa gcttgctttt cttgggtggg gtagttctgc
     1741 acgtttcacg cgatccacga gtttagttag cccaccgatt tccctcattc tcccattgga
     1801 cgttgtgagg taaacgaaaa atccccatcc aagcaaaacc agcttggatg gggcaccctc
     1861 aggattcaat tgtcggggct accgctacca ctagccacta acccctaggc ccctgctttt
     1921 tcacgcttgc gccggcaatc ctccatcgga aatccagcga gtcgatggtt tgacggttgc
     1981 cttgggaaga atgtcgagat agatgacggc ataccggaaa gcgattacgc cagccgcgat
     2041 gaccagaacg cttgacgcca actccgtcca cttgggcacg tagtgcgttc ccatggctga
     2101 ctgtaaggag ttgatcgaga cgttcagccg gtcaaccatg aatcctgcaa ccacgatggc
     2161 agaggtccag tacagccgct cgggattcga acgcacactc tgaaagctca ggaggaagat
     2221 ggggatcgcc aggaacagcg ccacctcgag ccagaagtat ccggcttcgc tggtgcgctg
     2281 gaaggcatag cccagcgcct ggttcgaaac caggtcgagg agtttgaaga aggcatacac
     2341 gatcagcaac agttggatca tccgg
//
LOCUS       DASRKK010000643         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_550043, whole genome shotgun sequence.
ACCESSION   DASRKK010000643 DASRKK010000000
VERSION     DASRKK010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550043"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1615
                     /locus_tag="VFC15_15730"
     CDS             341..1615
                     /locus_tag="VFC15_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM11657.1"
                     /translation="MAVTILLIVMAWQPIEAQTFATLHGFSGPDGATPTAGLTMDAAG
                     DLYGTTQYGGTAGYGSVFKLVHTGSTWILHQLYSFPSIYSGRNDGAEPYAGVTIGPDG
                     NLYGTNTLGSGRSNYGTVFKLSPPASVCKSTMCPWTETILYRFSGGSDGGYPYGPVVF
                     DSVGNLYGTTESGGMTCGQNFTCGVVFKLTRSGSGWTETVLHAFLNSPDGAIPASGLV
                     FDQAGDIYGTTSGGGTGLNCESNGCGTVFQLMPSGSGWTENVLHSFTYSNNDGAVPQG
                     GLISDSSGSLYGTTVFSPNNNGNGTVFELTPSGGQWTYTLLSSLPEMFFGITGPFGSL
                     VMDATGNLYGTTIGSGDAYGSGLCQYGCGTVFKLTPSAGAWTFSLLYEFAGGADGAHP
                     YDGGILDRNGNLYGTASTAGARGDGVVFEITP"
     gene            1997..>3174
                     /locus_tag="VFC15_15735"
     CDS             1997..>3174
                     /locus_tag="VFC15_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11658.1"
                     /translation="MSKLPQTLGELRSSSQFPEQRVRNRRVKDEMRDNLIARLRSGGP
                     IFPGIVGYDDTVVPQIVNAVLSRHNFILLGLRGQAKSRILRALAGLLDPQLPYLSGCE
                     IHDNPYAPICRRCRELLANNPDDAPISYLAADDRYVEKLATPDVTIADLIGDIDPIKA
                     AKGGHELGSEYTVHYGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPV
                     RMPLDVALVFSANPEDYTARGKIITPLKDRIGSEIRTHYPATIEEGIAITAQEAWTAR
                     TGLQLQVPEFVSQAIEQIAFIAREDKRVDKRSGVSQRLPISATENVVSNAERRAIRSG
                     EKLVVPRIGDIYAALPAMTGKLELEYEGELKGADNVVREIIRAAVARTYDKYFHGNSN
                     "
BASE COUNT          645 a          977 c          907 g          645 t
ORIGIN      
        1 gttagatctc cgctttacag gctgcttccg cggtgataag gttatgcccc attagggccc
       61 ctctggagac tgggagacgc gcatgactgg agttttcctt ccaagatttc gccctccaac
      121 ggcgcctgga tcgccacccc cttcgtcact tcacggtaag cgccacacgc aagatcgagc
      181 cgttagcgaa tagtggttgg tcatcagctc tataccacaa tgtgggacga agagtttgat
      241 gagaacacga aattggtccg aaaggagatt tactatggcg catcctatgc aacatcgcaa
      301 atggactggt ggggtggact tgaggatgac ggtcgcatcc atggcagtta cgattcttct
      361 gatcgttatg gcttggcaac ctatagaggc gcaaaccttc gccacgcttc acggcttcag
      421 tggccctgac ggcgcgacgc ccacggcggg actgacgatg gacgcagccg gggacctcta
      481 cggcacaacc caatacggcg gcacggccgg ttacggttcg gtcttcaaac tggtgcacac
      541 aggttccacc tggatcctgc accagcttta cagcttcccc agtatctaca gtggacgcaa
      601 tgacggcgct gaaccctatg cgggggtcac catcggtccc gacggtaacc tttacggcac
      661 gaatacttta gggagtggca gatctaacta tggcacggta ttcaaactct cgccgccggc
      721 ctcggtgtgc aagagcacta tgtgcccttg gaccgaaacc attctgtacc gctttagcgg
      781 aggcagtgac ggaggatacc cctacggtcc ggtcgtgttt gacagcgtcg gcaatctcta
      841 tggcaccacc gagtctggcg gaatgacttg tggccagaac tttacttgcg gtgtcgtctt
      901 caagctgaca cgatcggggt ccggctggac ggaaaccgta cttcatgcct ttctgaactc
      961 gccggatgga gcaattccag ccagcggctt ggtgttcgat caggctggcg atatctacgg
     1021 tacaaccagt ggcggtggca ccggattaaa ctgcgaatcc aacgggtgcg gcacggtttt
     1081 ccaactgatg ccctccggct ccggctggac cgaaaatgtt cttcacagtt ttacctatag
     1141 caacaacgac ggggctgtcc cgcagggcgg gcttatctcc gacagctcag gcagtctcta
     1201 tggcacgacg gtcttttcgc ccaacaataa cggtaacggg acggtcttcg aactcacacc
     1261 ctctggtggg caatggacct atacactgct ctccagctta ccggagatgt tttttggtat
     1321 cacgggcccc ttcggctcgc tcgtgatgga cgcgaccgga aatctctacg gcacgaccat
     1381 tgggtcgggc gacgcgtatg gctccggcct ttgccaatac ggatgcggta ccgtgtttaa
     1441 gctcacaccc tcggccggcg cctggacgtt cagcttgctc tacgaatttg ccggaggcgc
     1501 cgatggcgcc cacccgtacg acggcgggat tttggatcgg aacggcaatc tctatggcac
     1561 ggcttcgact gccggcgcga gaggggatgg cgtcgtcttc gagatcacgc cgtagctgaa
     1621 ttctgcggac ttggtggggt gtcatcctga gcgacgagcg tagcgaggag tcgaaggaac
     1681 cctatcgccg caagcaatcc caggcttctc tttgtgccgg gcggccacac tgtgatttct
     1741 tgcaaccata gagttccttc gactgcgcgc ccgcccgacc cgcaaagcag cagcccggaa
     1801 gttccttgcg ggcgctccgc tcaggatgac accttctcct tcaccccact gcttcctttc
     1861 ctgctgcgat aatcctcact gtgctgacgt tcattgcgcc gcgggcataa tcatcgctgg
     1921 cgtagaatac gaacattcgt taccattcgg tccgctgtgc gaggatggcg gccgcgaccc
     1981 tggaggtatc cagcccttga gcaaacttcc gcagaccctg ggcgagcttc gcagcagctc
     2041 gcagtttcct gaacaacggg tgcgcaaccg ccgcgtcaag gacgaaatgc gcgacaacct
     2101 catcgcgcgc ttgcgcagcg gcgggcccat cttccccggc atcgtcggct acgacgatac
     2161 cgtggtgccg cagattgtga acgcggtatt gtcgcggcat aatttcattc ttctcggatt
     2221 gcgcggacag gcgaagagcc gcatcctgcg cgcccttgcg ggcctgctcg atccgcagtt
     2281 accctacctg agcggctgcg aaatccacga caatccctac gcgccgattt gccgccgctg
     2341 ccgcgaattg ctggccaaca atcccgacga tgcgcccatt tcttacctgg ctgccgacga
     2401 tcgctacgtg gaaaagctag ccactcccga cgttaccatc gccgacttga tcggcgacat
     2461 cgatcccatc aaggcggcca agggcggcca cgaacttggc agcgagtaca ccgtccacta
     2521 cggactgttg ccgcgcgcca atcgcggtat cttcgccatc aacgagctgc ctgatctcgc
     2581 cggcaagatc caggttggcc tgttcaacat catgcaggaa ggcgacgtgc agattaaggg
     2641 ctacccggtg cgcatgccgc tcgatgtagc gctggtcttc agcgccaatc ccgaggacta
     2701 caccgcgcgc ggcaagatca ttacgccgct gaaggaccgc atcggctccg agattcgcac
     2761 gcattatccg gcgaccatcg aagagggaat tgccatcacc gcgcaagaag cgtggacggc
     2821 gcgcaccggc ctgcaactgc aagttcccga attcgtgagc caggcgatcg agcagattgc
     2881 gttcatcgct cgcgaagata agcgcgtcga taagcgctcc ggagtgagcc agcggctgcc
     2941 catcagcgcc acggaaaacg tcgtctccaa tgccgagcgg cgcgcgattc gctcgggcga
     3001 gaagctggta gtcccgcgta tcggcgacat ttacgccgcc ctgccggcga tgaccggcaa
     3061 actcgaactg gaatacgaag gcgaactgaa gggcgccgac aatgtggtgc gcgagatcat
     3121 tcgcgccgct gtcgcccgca cctacgacaa atattttcac ggcaacagca acat
//
LOCUS       DASRKK010000644         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1039141, whole genome shotgun sequence.
ACCESSION   DASRKK010000644 DASRKK010000000
VERSION     DASRKK010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039141"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFC15_15740"
     CDS             <1..688
                     /locus_tag="VFC15_15740"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZM11659.1"
                     /translation="KHLGGMTYGGYSRTIVVDEAFVLRPPANLDLAGTAPLLCAGITT
                     YSPLRRWKVAKGQRVGIVGLGGLGHMGVKLANAFGAHVALFTTSAHKTADGKRLGAHE
                     VVVSKDPAQMEKHTNSFDFILDTVSAEHDINAYLNLLKRDGTLVLVGAPEKPLALSAF
                     SIIPGRRQLSGSMIGGIPETQEMLDFCGEHGITSDIEIIPIQKINEAYERMLRSDVKY
                     RFVIDMASLK"
     gene            complement(905..1159)
                     /locus_tag="VFC15_15745"
     CDS             complement(905..1159)
                     /locus_tag="VFC15_15745"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HZM11660.1"
                     /translation="MKKKVPRLKTDKEAEAFLAHDLSDLDFSQFKPARFEFEKKDEQI
                     NMRVPRTLLAALKARAKARDIPYTRFIREALEQALTAPKS"
     gene            complement(1156..1428)
                     /locus_tag="VFC15_15750"
     CDS             complement(1156..1428)
                     /locus_tag="VFC15_15750"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM11661.1"
                     /translation="MTGFDWDGGNRAKCQKHGISIAEIEALFRNAPRIAPDPRHSEDE
                     DRMIAVGRTGAGQPVFVAFTLRTKNRRRLIRPVTALYMHAKEIAAV"
     gene            complement(1535..2575)
                     /locus_tag="VFC15_15755"
     CDS             complement(1535..2575)
                     /locus_tag="VFC15_15755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZM11662.1"
                     /translation="MATTATPQPKSNRIGLTVLDYRGGKTTLCAGCGHNAISERIIDA
                     FYEMGVAPERVVKMSGIGCSSKSPAYFMGRSHSFNSVHGRMPSVTTGAVLANSKVVAL
                     GVSGDGDTASIGIGQFMHLMRRNLPMIYIIEDNGVYGLTKGQFSATADLGSKLKTGVI
                     NDLPPIDTCALAIMLGATFVGRSFSGDKRQLHTMLKAAIAHRGTVMLDVISPCTTFND
                     HEGSTKSYKFVKDHEEPLHDLNFVPHFNEIDVDYDPGTSLNVTMHDGSSLRLHKLEKD
                     YDPTNKTEAVKRVMESHEAEEVLTGVLYLNTTAPNFIDMINMTDQPLATLPESVTRPS
                     REVLDECMEALR"
     gene            complement(2576..>3385)
                     /locus_tag="VFC15_15760"
     CDS             complement(2576..>3385)
                     /locus_tag="VFC15_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11663.1"
                     /translation="QGDIEFVAKLSHGDTHHPMLLPCSVAECFSMASDAFDLTEQLQT
                     PVFVMSDLDLGMNNWMSEPFQYPEKPQNRGKVLTAEDLERAGGFARYKDVDGDGVGYR
                     TLPGTDHPQAAWFARGSGHNEKSQYSERPDDYANNMDRLAHKFETARTLVPKPEMVRN
                     DGNEVGIIAYGTSHHAVVETLDQLQNEYGVKTDYLRLRAYPFTTETHDFVAAHKRTYM
                     IDQNRDAQMLQLLKLDIAIEEISKLRSVRHYNGLPIDARSITDNIIVQESK"
BASE COUNT          658 a          978 c         1022 g          727 t
ORIGIN      
        1 caagcatctc gggggcatga cgtacggcgg ctattcgcgc acaatcgtgg tcgacgaggc
       61 atttgtgctg cggccacccg cgaacctgga tctcgcgggg acggcgcctc tgctttgcgc
      121 cggcattacg acctactctc ctctgcggcg ttggaaagtc gcgaagggcc agagggtcgg
      181 catcgtggga ttgggcggat taggccacat gggagtgaag ctggctaacg cattcggcgc
      241 gcatgtcgcc ctgttcacca cttccgctca caagaccgcg gacggcaagc gcctcggcgc
      301 gcatgaagtg gtggtgtcca aagatccggc gcagatggag aagcacacca acagtttcga
      361 cttcatcctc gacaccgttt cggccgagca cgacattaac gcttatctga acctgctgaa
      421 gcgcgacgga accttggtgc tggtgggcgc gcccgaaaag ccgctggcgc tgtccgcatt
      481 cagcatcatt cctgggcggc ggcagctctc gggctcgatg attggcggca ttccggagac
      541 gcaggagatg ctcgatttct gcggcgagca cggcatcacg tcggatatcg agatcatccc
      601 gatccagaag atcaacgaag cctacgagcg catgctgcgc agcgatgtga agtaccgctt
      661 cgtgatcgat atggcgtcgc tgaaatgaaa cctctccgcg acggtggagg cgtcggggcc
      721 tctcttccgt accatctctt tcgtgatggg aagctgcgaa aaaccccacc caagcaaaaa
      781 aaaacttggg tggggcaccc ggccccggaa catttgccat tgatggcgag gattctagta
      841 ggcgatccgt ttcatgcggt gcccgcaatg cgagctgatt ggaaattgaa acacggcgag
      901 aacgtcagga cttcggtgcg gtcagagcct gttcgagagc ttcccggata aagcgcgtgt
      961 agggaatgtc ccgcgcctta gcccgcgcct tgagcgctgc cagcaacgtc ctgggcacgc
     1021 gcatgttgat ctgctcgtcc tttttctcaa attcgaaccg cgcgggtttg aactgcgaaa
     1081 aatcaaggtc cgacaggtcg tgcgccagaa acgcttccgc ttccttatcg gttttgagcc
     1141 ttggtacttt ctttttcata cggcggcgat ctccttcgcg tgcatataga gggccgtcac
     1201 cgggcggatg aggcggcgtc ggtttttcgt ccgtagggtg aaggccacaa acacaggttg
     1261 cccggcgccg gttcttccca cggcgatcat gcggtcctcg tcctccgagt gtctcgggtc
     1321 gggcgcaatg cggggagcgt tcctgaacag cgcctcgatc tccgcgatgg aaatgccatg
     1381 cttttggcac ttcgcgcggt tgccgccgtc ccagtcaaat cccgtcaccc gcaaattcac
     1441 catagcagaa agctatacaa atgtatatac aaatgtcaac gggcaaatgc tcctatcgtc
     1501 gggatgacaa acatcggggg cgaggaactt cgccttaccg cagcgcttcc atgcactcgt
     1561 ccagcacttc gcgcgagggg cgagtgacgc tctccggcaa cgtggccagc ggctgatcgg
     1621 tcatgttaat catgtcgatg aagttgggcg cggtggtgtt caagtagagc actccggtga
     1681 gcacttcctc ggcctcgtgc gattccatca cgcgcttcac agcctcagtt ttgttggtgg
     1741 ggtcgtaatc cttctccagc ttgtgcagcc gcagcgagga gccatcgtgc atggtgacgt
     1801 tgaggctggt gccgggatcg taatccacat caatttcgtt gaagtgggga acaaagttca
     1861 aatcgtgcaa cggctcttcg tggtccttga cgaacttgta ggacttggtc gagccttcgt
     1921 gatcgttgaa ggtggtgcaa ggcgagatca cgtcgagcat cacggtgccg cgatgcgcga
     1981 tcgcggcttt cagcatggtg tgcaactggc gcttgtcgcc agagaaggag cggcccacga
     2041 aggttgcgcc cagcatgatc gccagagcgc aggtatcgat tggcggcaga tcgttgatga
     2101 cgccggtctt cagcttggag ccgagatcgg cggtggcaga gaactgcccc ttagtcagcc
     2161 cgtacacgcc attgtcttcg atgatgtaga tcatcggcag attgcgccgc atcagatgca
     2221 tgaactggcc gatgccaatg gacgccgtat cgccgtcacc gctgacgccc agcgccacaa
     2281 ccttggagtt cgccagcacc gcgccagtcg taaccgacgg catgcggccg tgcaccgagt
     2341 tgaagctgtg cgagcggccc atgaagtacg ccgggctctt cgaggagcaa ccgatccccg
     2401 acatcttgac cacccgctcg ggcgcgacac ccatctcgta gaaggcgtcg atgatacgtt
     2461 cagagatggc attgtggccg catccggcgc acaacgtggt cttgcctccg cgatagtcga
     2521 gcaccgtcaa tccgatgcgg ttcgacttgg gctgaggagt ggctgtggtc gccatttatt
     2581 tgctctcctg gacgatgatg ttgtcggtga tggaacgcgc gtcgatcggc aatccgttgt
     2641 agtggcggac gctgcgcagc ttgcttatct cttcgatcgc gatgtccagc ttcaataatt
     2701 gcaacatctg cgcgtcgcgg ttctgatcga tcatgtaagt gcgcttgtgc gcagccacga
     2761 agtcgtgcgt ctcggtggta aacggataag cgcgcaggcg caggtaatcg gtcttcactc
     2821 cgtattcgtt ttgcaactga tcgagggtct caacgacagc gtgatgcgag gttccgtagg
     2881 cgatgatccc aacttcgttg ccgtcgttgc gcaccatctc cggcttggga acgagcgtac
     2941 gcgcagtctc aaacttgtgc gccagacggt ccatgttgtt cgcgtaatcg tcgggccgct
     3001 cactgtactg cgacttttcg ttatgtccgc tgccgcgcgc gaaccacgcc gcctggggat
     3061 ggtcggtgcc cggcaaggtg cgatagccga cgccgtcgcc gtccacgtcc ttgtatcgcg
     3121 cgaaaccgcc cgcacgttcc aggtcttccg cggttaacac cttaccgcga ttctgcggct
     3181 tctcgggata ctgaaacggt tccgacatcc aattgttcat tcccaaatcg aggtcagaca
     3241 tgacgaacac tggcgtctgc aactgctcgg tgaggtcgaa agcgtcgctt gccatgctga
     3301 agcactcagc cacgctacaa ggcaacaaca tgggatgatg agtgtcgccg tgggagagct
     3361 tggccacaaa ctcgatgtcg ccctg
//
LOCUS       DASRKK010000645         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1136583, whole genome shotgun sequence.
ACCESSION   DASRKK010000645 DASRKK010000000
VERSION     DASRKK010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136583"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="VFC15_15765"
     CDS             <1..1654
                     /locus_tag="VFC15_15765"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM11664.1"
                     /translation="TPGGLMQPAAPPLDVTPEELEALLEGVREALGEAGYQKLKAAIR
                     TLSYVTQLLENREATLQTLRRLLCQARTEKTEAVLQQAGIEGSPTKRRPAAAAAAKHP
                     AAGHGRNGAEAYRGARRVEVQHASLHHGDRCPECQRGKVYPLHDPGLLVRMKGQAPIA
                     ATVYALEKLRCNLCGETFTAVAPEGVGEEKYDATTASMIALLRYGSGFPWNRLERLEE
                     SLGIPLPAATQCEIVAEAAAAIQPAMAELIGHAAQGDVLHNDDTSMPVLALRRDARHE
                     DVHTEDDATERTGVFTSGIVSTAHGQHIALFFTGRQHAGENLADVLKQRAAELAPPIQ
                     MCDALSRNLPKLPEKLEVIVGHCLAHARRRFVEVTPNFPEECRHVLEQLGEVYSHDAR
                     AREQGMMPEERLCLHQLHSGPVMDQLHVWLTAQFAEKKVEPNSGLGMAIRYLLKHWER
                     LTLFLRQAGAPLDNNICERALKKAILRRKNSLFYKTENGARVGDLFMSLIHTCELCGA
                     NPFDYLTELQRHAAELARNPSPWMPWNYRATLPQSGIGVDSG"
     gene            2336..3820
                     /locus_tag="VFC15_15770"
     CDS             2336..3820
                     /locus_tag="VFC15_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11665.1"
                     /translation="MRNKPTVALSLFLALAMTLLAGCGSSFSPKSSEPPTPAPLSSGN
                     LNLIFVVSEDLAYQASGDVNPTTANLTNQGLQRSLLMATFLQQQVLGTQNVTGIYALE
                     PMTHLQTANNYPDMAALETIQQFAMLNQTSASYQGSGPVTANSYPIFASYSLEAALPD
                     DVAPPAFPCAACQGLDFRDQNRDNEALVTGIVKANAPGFYVFSAPWETTSSLLANINK
                     LEGYNLSLPASYISPNYIYAISIAPSGSASLVTYNSYLTPPSTYPVLPPLVSTPCNTP
                     QTQAFFSRTVTGGIDGAVIPAGTNTNETVYFVRHADAHPTSWWDDGNYIGKGQWRALD
                     LPNALRDKIHPTQVYAIDPAVGNPGGEGSVASSYVRPSLTVEPYAIANNLPFNLAASV
                     PVFSQIPPQPSTDASIYFFNGGTFSNQTILVGWEHDHIPPTVNALLTSYNAGPLAPPS
                     DKWPDTDYDTIWTVKLDAVGNLTVNNGMCEGIDSAALPPTPPQF"
     gene            complement(3828..>4047)
                     /locus_tag="VFC15_15775"
     CDS             complement(3828..>4047)
                     /locus_tag="VFC15_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11666.1"
                     /translation="SWRQFTIRQLSGISAHPESEPTSQIVASQLPELKLWVIESNTGR
                     LPFPQLLMAEMMTGPSSAISYPSGAAAA"
BASE COUNT          857 a         1214 c         1203 g          773 t
ORIGIN      
        1 tactccagga ggcctgatgc aaccggcagc accgcccctc gacgtcaccc ccgaagagtt
       61 ggaggcgttg ctggagggcg tgcgggaagc gctgggggag gccggctatc agaaactgaa
      121 agccgcaatc cgcacgctga gctacgtgac ccagttgctg gagaaccgcg aggcgacttt
      181 acagaccctg cgccgcctgt tgtgtcaggc gcgcacggag aagaccgagg cggtgctgca
      241 gcaggcgggc atcgagggta gtccgacgaa acggagaccg gcggcggcag cggctgcgaa
      301 gcatccggcc gccggacacg ggcgcaacgg agcggaagct tatcgtggag cacgccgggt
      361 cgaggtgcag catgcgtcct tgcaccacgg ggatcggtgc ccggagtgtc agcgcggcaa
      421 ggtgtacccg ctgcatgatc ctggactatt ggtgcggatg aaaggccaag cgccgatcgc
      481 agccacggtg tatgcgttgg aaaagctacg ctgcaatctg tgcggggaaa cgttcacggc
      541 ggtggctccc gaaggcgtgg gggaggagaa gtacgacgcg acgacggcga gcatgatcgc
      601 gctgcttcgt tacgggagtg ggtttccgtg gaatcgattg gaaaggctgg aggagagtct
      661 gggcattccg ctgccggccg cgacgcaatg cgagatcgta gcggaagcgg ccgccgcgat
      721 ccaaccggcg atggcggagt tgattggcca cgcggcgcaa ggcgacgtac tgcacaacga
      781 cgacacgtcc atgcccgtgc tggccctgcg acgggatgcg cgccatgaag atgtccatac
      841 ggaagacgat gcgacggagc gcacgggagt gttcaccagc gggatcgtat cgaccgcgca
      901 cgggcaacat atcgcattgt tcttcaccgg acgccagcac gcgggggaga acctggccga
      961 tgtgctcaaa cagcgggcgg cggaactcgc tcctcccatc cagatgtgtg atgcgctgtc
     1021 ccgcaaccta cccaaactgc cggagaaact ggaagtcatc gtgggccatt gtctggctca
     1081 cgctcgccgc cgctttgtgg aggtgacgcc caactttccc gaggaatgcc ggcatgtact
     1141 ggaacaactg ggcgaggtct acagccacga tgcacgggcg cgggagcaag gtatgatgcc
     1201 ggaagagcgg ttgtgcctcc accagcttca tagcggcccg gtgatggacc aactacacgt
     1261 ttggctcacc gcccagtttg cggaaaagaa agtggagccg aactccggtc tgggaatggc
     1321 gatccggtat ctgctcaagc actgggagcg gctcacgcta tttttgcggc aggccggagc
     1381 cccgctggac aacaacattt gcgaaagagc attgaagaaa gcgattctgc gtcgcaagaa
     1441 ctctctgttc tacaagaccg agaatggggc ccgtgtcggc gacctgttca tgagcctgat
     1501 tcatacctgc gaactgtgtg gcgccaaccc gttcgattac ctcaccgagc tacagcggca
     1561 tgccgccgag ttggcgcgga atccctcacc atggatgcct tggaactacc gcgcgacgct
     1621 tccacagtct ggcatcggtg tggattccgg gtagagtatg atgaatcccg cctggccgga
     1681 aacaatcgat tccggccata cacaggagcg actcgaagat ggccggaaaa ccggcctcta
     1741 gcaaagccga ttcgcagcag gtttttggcc agggggctgg ctggaggaaa actccgcgcc
     1801 gtgctggcct tgtacgcccg cgtctctacc acggagggca gactctgcca ttctagagcc
     1861 atgccatttt caaatgggac cggtgacaat agtccagtcg tctaaggacc tggccagatt
     1921 gctatgcctt ttcccatttt gcaccctcca cgagtggtta ctttcgtgcg aagacgtcct
     1981 tgacatcaca gacaccaagg cacttgcctg ccataggatc ctatgtgtgg acatgctggc
     2041 agaacccgcc ccttgtgtcc ttatccgaag tttgctcaat acatgggcgg cccagcaggc
     2101 agcgaagatg ccgcgagttt cacgtgtgta gcgcccattg actaaacgca gagtattgag
     2161 ttcgccgaaa ggcgttcgtc tgttagcgcg cgaaaatccc cgcggttagg catccggaat
     2221 tggaaaatcg cccttttcga gatttgcccg ggttttttac tcgcccgcgg tggctgatgt
     2281 cactgccaag cgattgccgc aagcatgaaa tggttgtaag aagaggctcc aagctatgag
     2341 gaacaaacct accgttgcac tgtcgctgtt cttggcgctt gccatgacac tgctcgcagg
     2401 atgcggcagc tctttcagcc ccaagtcatc agagccgcca actcccgctc cgctgagctc
     2461 cggcaacctc aacctgatct tcgtggtcag cgaggatttg gcttaccaag cttcaggaga
     2521 cgtcaatcca acgacggcaa acctcaccaa tcagggctta cagcggtcgc tcctcatggc
     2581 cacgttcctg caacagcaag tactgggaac gcagaatgta actggcattt acgcgctcga
     2641 accgatgacc catttgcaaa ctgcaaacaa ttatcccgac atggcggcgc tggagaccat
     2701 tcagcagttt gccatgctga atcagaccag cgcgtcgtac cagggcagcg ggccggttac
     2761 cgcgaacagc tatcctatct ttgcatcgta ttcgttagag gccgcattac cggacgacgt
     2821 tgcgccgccg gcgtttccct gcgcggcttg ccagggtctc gacttccgcg accagaaccg
     2881 cgacaacgaa gccctggtaa ccggtatcgt gaaggcaaac gcgccggggt tttatgtctt
     2941 ctcggcgccg tgggagacga ccagcagcct gctggcaaat atcaataagc tcgaaggcta
     3001 caacctgagc cttccagcga gctatatcag tcccaactat atctatgcga tttcgatcgc
     3061 gccgtcggga agcgccagcc tggttaccta caacagctac ctgactccgc cttccaccta
     3121 ccctgtgctg cccccgctgg tcagcactcc ctgcaacacg ccgcagacgc aggcgttttt
     3181 cagcagaaca gtgaccggcg gtattgatgg cgccgtaatc ccggcgggga ccaacaccaa
     3241 cgaaacggtt tactttgtcc ggcacgcgga cgcgcatccc acgagttggt gggacgacgg
     3301 caactatatt ggcaaggggc aatggcgcgc cctggatctt ccgaacgcat tacgcgacaa
     3361 gatccatccc acgcaggttt acgccatcga tcccgcggtc ggcaatccgg gcggtgaagg
     3421 tagtgtcgct tcgtcgtatg tcaggccttc gttgaccgtg gaaccgtatg ccatcgccaa
     3481 caacttgccc ttcaatctgg ccgccagcgt ccccgttttc tcccagattc cgccccagcc
     3541 atccaccgac gccagtattt atttctttaa cgggggcacg ttctctaacc aaacgatact
     3601 ggtgggctgg gagcacgatc acatcccacc gaccgtaaat gcgctgctca ccagctataa
     3661 cgctggccca ctggctccac cttccgacaa gtggccggac accgactacg acaccatctg
     3721 gacggtgaag cttgacgctg tgggcaactt gacggtgaac aacgggatgt gcgaaggcat
     3781 cgattccgcg gcgctgccgc ccacgcctcc tcaattctga cggtcgctca ggcggcggcc
     3841 gcgccactcg gatatgaaat cgccgagctc ggccccgtca tcatctcggc catgaggagc
     3901 tgcgggaatg gtaagcgtcc ggtattgctt tcaattaccc acaacttcaa ctccggaagc
     3961 tgcgacgcca ctatttgcga agtaggttcg gactcagggt gtgcacttat gcccgagagt
     4021 tggcggatgg tgaactgccg ccatgaa
//
LOCUS       DASRKK010000646         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1431165, whole genome shotgun sequence.
ACCESSION   DASRKK010000646 DASRKK010000000
VERSION     DASRKK010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431165"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1777)
                     /locus_tag="VFC15_15780"
     CDS             complement(290..1777)
                     /locus_tag="VFC15_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM11667.1"
                     /translation="MRDTPVTAPKVAVRFAPEDLDLFRDASGDRNPIHCSADYASRTV
                     YGERLVYGALGAIACLGHIDLQPDVRIAKLVADFQRSMFCGVDYTVHRIEGPPFCVRL
                     MDGLLPVVTVSLWKDSSARKGFASTGICQSHSYWNLSEPVCLKEEEIQPGLRVSGRYH
                     CDPTALVHLCKRWAVSAPASLVQTLLWGSYFTGMDLPGRSALFFRLSLDFSDHQANDD
                     TCEVLDYEAVVTKVDPRLSQVRCQATLKDTSNQSIKAECISFVRPSLDNPSNSATELS
                     NDLVGKIALVIGGNRGLGAEITRCLVSHGCTVISTSRSPGAQNGVNTFSGSAADAAWL
                     QTVRDAIQKQHGKLDFLICNAFPAINPLRMESNAIERIQAYLQQATALVLNPLCVFME
                     MLDASGGRAVVVSSTATQRPVKEWPHYVAAKKAIEGLAQVAALQYPRVQTMIVRPNKL
                     LTEMINTPMGRKNAEPPWKVASKLVDHLRGPAQPGITVLEFPEPA"
     gene            complement(1808..2050)
                     /locus_tag="VFC15_15785"
     CDS             complement(1808..2050)
                     /locus_tag="VFC15_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZM11668.1"
                     /translation="MDDTRKRLTSCFQVVFPDLTEAEIPVASQERVAAWDSLAAITMV
                     SVIEDEFALQMDLDDLDDLDSFAKVHAYLQKRLQVA"
BASE COUNT          498 a          613 c          618 g          456 t
ORIGIN      
        1 cagtccctgc atgaatatga gctgagcgtt aaatcctaac ctcagcaggt ggttgagaaa
       61 tctgggtaga ggcagagtta ctcgacacga tgcggcgagc ggtatcgcgc agccaacgat
      121 agaagggggt ctcgccccgt cggattttaa gcatcaccga gctcattgct gatctatccc
      181 tggagtctct ctttctgtca gtgtaggaac agaatgggga actgcttgcc aggctcctct
      241 ctccatcata cggcgaaaag aatctgccct cgactctatg ccatccggcc taagcgggct
      301 ccggaaattc cagcacggtt atacctggct gggccggtcc gcgcaaatgg tccaccagct
      361 tcgacgccac tttccaaggc ggttcggcat tttttctccc cataggcgta ttgatcattt
      421 ccgtcaaaag cttgttgggg cgaacaatca tggtctggac acgagggtac tgcagggccg
      481 ctacttgggc caaaccctca atggctttct tggcagcaac atagtgaggc cattctttga
      541 ccggccgttg ggtcgcggtg gaggacacaa cgaccgcgcg gccgccgctc gcatccagca
      601 tctccataaa cacgcacagc gggttgagca ctagggcggt tgcctgttgc agatatgcct
      661 ggatgcgctc aatcgcgttg gactccatcc gcaggggatt gattgctgga aatgcgttgc
      721 aaatcaggaa atccagcttg ccatgctgct tttggatcgc atcacgcaca gtctggagcc
      781 aggccgcatc ggccgcgctt cccgagaaag tgtttacgcc attttgagcg cccggacttc
      841 gcgatgtgga gatcacagtg cacccatgtg aaaccaggca gcgagtaatc tctgcgccca
      901 ggccccggtt gccgccaata accagcgcta ttttcccgac taaatcattc gaaagctcgg
      961 tcgctgaatt gctgggatta tcgagcgacg gtctgacgaa agaaatgcat tccgctttga
     1021 tggactgatt tgaggtgtct ttcaaagttg cttggcaccg cacttggctg agacgcggat
     1081 caactttggt gacgacggcc tcatagtcga gaacttcaca ggtatcgtcg ttcgcctggt
     1141 ggtcgctgaa atcgagcgag agccggaaaa atagagcgga ccgtccgggc aaatccatgc
     1201 cggtgaaata actaccccac agaagtgtct gtaccaggga agccggcgcc gaaactgccc
     1261 agcgcttgca caggtggaca agggctgtcg gatcgcagtg ataccgaccc gagactctca
     1321 ggccaggttg gatttcttct tcctttaggc aaacgggttc agagaggttc caataactgt
     1381 gggactggca gatgccagtc gatgcgaagc ccttccttgc cgaggaatcc ttccagaggc
     1441 tcacagtcac aaccggtagc agaccgtcca tcaatcgcac gcaaaacggg ggaccttcaa
     1501 tgcgatggac cgtatagtcc acaccgcaga acatggaccg ttggaagtcg gccaccagct
     1561 ttgcgatgcg cacgtccggc tgaagatcga tgtgaccaag acaggcgatg gcgcccaagg
     1621 ccccgtaaac caggcgctcg ccataaaccg tgcgagaggc gtagtccgct gagcaatgga
     1681 tcgggttccg gtcgccggat gcatcccgaa agagatccaa atcttctgga gcgaaacgga
     1741 cagcaacctt aggagcggtc acgggagtgt cacgcataga agtgagcgac gcgcaatgga
     1801 atcaaggtca ggccacctgc agccgtttct ggagataggc gtggaccttc gcgaaggaat
     1861 ccaaatcgtc caaatcgtcc aaatccattt gcagggcgaa ctcgtcctcg attacgctta
     1921 ccatggtaat cgcggcaagg gagtcccagg cggcgaccct ctcctgcgag gctaccggaa
     1981 tttcggcttc cgtcaaatcc ggaaacacga cttggaagca actggtcagt cgctttctcg
     2041 tatcatccat gggtttctgc tctcctcaac tcgcggaccg cgtgatagag cgcgggacac
     2101 gactcatcga attgtgcacg actcagccgg aactccgtcg tgggttgctc gccagtgata
     2161 gctgcaagaa actcctgcag tgggc
//
LOCUS       DASRKK010000647         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1235091, whole genome shotgun sequence.
ACCESSION   DASRKK010000647 DASRKK010000000
VERSION     DASRKK010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235091"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFC15_15790"
     CDS             complement(<1..798)
                     /locus_tag="VFC15_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZM11669.1"
                     /translation="MIKDVILHLERDQSYDQVRDSALSIAETLDAHVTGVAFAYDPDA
                     NFPAQMLAEMRAESEKGARATIARFEAAAQRSLISSESCLVVENELRFPSTYSSLARR
                     FDLNILMQSVPGGANNDALIEASLFGSGRPVVVIPYIQKGILKLDRLVCCWDGSGFAA
                     RAINDALPLLEKASNVELLIVLNDRGDSAQAEIGGIEMAKHLARHKAKVDVKISRVGG
                     IDVTSAILSHAADCSADLIVMGGYGHSRLREFILGGATRGILSSMTVP"
     gene            complement(876..1532)
                     /locus_tag="VFC15_15795"
     CDS             complement(876..1532)
                     /locus_tag="VFC15_15795"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZM11670.1"
                     /translation="MSDSQLRQDIIDELEFDPSFNGEHIGVVVDNNVVTLTGHVNSYA
                     EKLAAIAAVQRIKGVHAIAESIEVRYPYQHKTADDQIAKRASDIINWDVHIPSSAVDV
                     LVHDHGWVTLSGTVDWYYQRREAEDDVRKLSGVRGVTNKIAIKPHVESSDVKSKIESA
                     LKRHAEVEASAIRITVQSGNKVVLEGNVDNWDERRKVENAAWSTPGVTSVDDRLTIKI
                     "
     gene            2801..>3643
                     /locus_tag="VFC15_15800"
     CDS             2801..>3643
                     /locus_tag="VFC15_15800"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HZM11671.1"
                     /translation="MERRTGSADLPLHGGRVPPWLATRMSSLGAIITQAIVHHYGRDE
                     FLQRLSHPFWFQSFGAVMGMDWHSSGITTSVIGALKRGLGPLSNELGIHVCGGRGQHS
                     RKTPDELRLLGERIGFDGAKLTRASRLVAKVDSAAVQDGFDLYLHGFFVTDDGKWTVV
                     QQGMNGDKKQARRYHWHSENLKSFVDEPHSAIDGPAQGEIVNLTDKRAENSRAKQLDL
                     LDVLGPDRIVSELAALTGETPAQALLPHLIMPAHHDVRSSDVFTRRLHGTLAAAAERG
                     PADFP"
BASE COUNT          769 a         1052 c         1031 g          791 t
ORIGIN      
        1 gggaactgtc atactggaaa gtattccgcg cgtggcgccg ccgaggatga attcacgcag
       61 gcgcgaatga ccgtaaccac ccatcacaat caggtcggca gagcaatcgg cagcgtgaga
      121 caggattgcg ctggttacgt caataccgcc gacccggctt attttgacgt cgaccttagc
      181 cttgtgtcgt gcgagatgtt ttgccatttc gatgccgcca atttcagctt gcgcgctatc
      241 gcccctatcg ttgagaacga tcaaaagctc gacgttcgat gccttttcca acaggggtaa
      301 tgcatcattg atggcgcgtg cggcaaagcc acttccgtcc cagcagcata cgagccggtc
      361 caactttaag atacccttct gaatataggg gatcacgacc actggacgac cggagccgaa
      421 caacgacgcc tcaatcaagg catcgttgtt cgcaccaccg gggacggatt gcatcagaat
      481 gttaagatcg aaacgccgtg caagacttga atatgtgctg ggaaaccgga gctcgttctc
      541 caccaccagg caagattcgc tggaaataag gctgcgctgt gctgccgcct cgaaccgcgc
      601 aatcgtagcg cgtgcaccct tctcgctctc ggcgcgcatc tctgccagca tctgggcagg
      661 aaagttggca tcgggatcat acgcaaacgc gacccctgta acatgggcat ccagtgtctc
      721 cgcaatggaa agagcggagt cacgaacctg atcgtaagac tggtcgcgct ccaagtggag
      781 aatgacgtct ttgatcatag cattgctccc ggtaactgcc cgtgtggcag caacagcatc
      841 gacgtcacct aacccaggat ttataagttg cgcctctata ttttaatcgt caagcgatca
      901 tcgacggatg taaccccggg agttgaccag gccgcatttt caaccttgcg gcgttcatcc
      961 cagttgtcca cgttcccttc caagacgact ttgttaccgc tctgtacggt gatgcgtatc
     1021 gcgctcgctt caacttctgc gtgccttttg agcgctgatt cgattttact cttcacgtcc
     1081 gaggactcca cgtgtggctt gatagcgatc ttgtttgtca cgccgcgtac accagacagc
     1141 tttcgcacgt catcttcagc ttctcttctt tggtagtacc agtcaaccgt tccgctgagc
     1201 gtcacccagc cgtggtcgtg gactagaaca tcgacggcgc tggaaggaat atgaacatcc
     1261 caatttataa tatctgaggc ccgcttagcg atctgatcat ccgcagtttt atgctgatag
     1321 ggatagcgga cctcgatgct ttcggcgatt gcgtgcacgc ctttgattcg ctggaccgca
     1381 gcaatcgcag cgagcttttc cgcgtaacta ttgacgtgcc ctgtgagagt tacgacatta
     1441 ttgtcgacca caacaccaat atgctcgccg ttgaagctcg ggtcgaattc gagctcatca
     1501 atgatgtctt gcctaagttg agagtcagac atgatggtcc tccattcagt tgatctttta
     1561 attgaaatta tcctctgccc tcggcaaggc gacgacattg atccgaatca aggagccgac
     1621 ctgccctgac ttagcagggc catatacatt atcgccagac gtgacgggtt cgggacgata
     1681 ctgcgctgat gccatgtttc gccaggcgag cagccgtcgt ttcgctcatg tccaacgagc
     1741 gctaagtgga tctcgataga aaagtttgaa tcggcaatgt gcgcgtctta agtttggtcg
     1801 gtcgcggcct gtccaagggc tgcagccgtt tgatagccca aggcgggaac ttatgcggac
     1861 tgattgcgtt gccctttgtt gtaggaggtt cgcatgcgta accgccgaac agcagttttt
     1921 atcttcgcag tcgtgctggc agttgtaatc gccgctgcgt tcataactac ccttcatggt
     1981 gtagatacaa cgcgggtggc caataacgag agtcctcccg gtgtcaccgg gttatctcaa
     2041 cctcacgctc cgctggatag cgcaccgggt agagcggtac agaactgatt gccgggtatc
     2101 tcttcgtcgg ttgttaccgc acgcgcgccc tcacacgacg cgtgtatgaa gagcaggaca
     2161 tcaagggcgc aaccattggt aaagccgtag aaacgcaaag ctatcgctga tcatccgctc
     2221 aggggattgt gaacggcgct gtttgataga gccgtgaatc tcgcatcgcc aagtaattga
     2281 tcggttcaaa agtcaggtgc gacgccaagc aaaggctcgg cggatttgct cattcttctc
     2341 cggacaggtc acgccgaacg catcagccta gtgcggtata tcctgcgccg gcggcaggag
     2401 gccgatccga tcccggagtg gctaggccag ttaatcgagc aggtcgaagc tttcggcggt
     2461 ccaacaacgc ggattggcca ggtcctttgc accgagtatg acgtcggggt cggcatcagc
     2521 tggcaccgag acaagccgca cttcgataag gtgttcggcc tgtcgctggc tcggcctgca
     2581 agttccggtt tcgaagaccg gccgacaaga agtggaaacg gttcacgctg gaggctcggc
     2641 cacgttcaat ctacatgatg tcggggccgt cgcgggaggt ctgggaacac agccgtcgag
     2701 gcgcagcgtt attcgatcac gttccggacg atggccgcca aatcatgacg ggccgcggaa
     2761 cgtctgctac catctcccgt atttggagtc gcaacagcga atggagagac gtaccggaag
     2821 cgccgatcta ccgctgcatg gcggacgcgt cccgccgtgg ctggcgacgc gcatgtcgtc
     2881 gctcggcgcc atcattacgc aggcgatcgt acaccactac ggccgcgacg aattcctgca
     2941 gcgcctgtcg cacccgttct ggttccagtc gttcggcgcc gtcatgggca tggactggca
     3001 ttcgtccggc atcaccacca gcgtcatcgg cgccctcaag cgcggcttgg gacccctctc
     3061 gaacgaactc ggcatccatg tgtgcggtgg acgcggccag cattcaagaa agacgccgga
     3121 cgagttgcgt ctgctaggcg aacgcatcgg cttcgacggc gccaagctga cgcgcgcgag
     3181 tcgactcgtt gcgaaggtcg acagtgccgc cgtccaggat ggattcgacc tttacctgca
     3241 cgggttcttc gtgaccgacg atgggaagtg gaccgtcgtg cagcagggta tgaacgggga
     3301 caagaagcag gcgcgccgct accattggca ttctgaaaac ctgaagagct tcgtcgacga
     3361 gccgcacagc gcgatcgacg gaccggctca aggcgaaatc gtaaacttga cggacaaacg
     3421 ggctgagaac tcccgcgcaa agcaactcga ccttttggac gttttgggtc cggatcgcat
     3481 cgtgtccgaa ctggcggcgc tgacgggtga aacgccggca caggctttgc tgccgcacct
     3541 catcatgccg gcgcatcacg acgtgcgctc aagcgacgtc ttcacgcgac ggctccatgg
     3601 cacgctcgcc gcggccgccg agcgtggtcc cgccgacttt ccc
//
LOCUS       DASRKK010000648        13205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1235151, whole genome shotgun sequence.
ACCESSION   DASRKK010000648 DASRKK010000000
VERSION     DASRKK010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235151"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..914
                     /locus_tag="VFC15_15805"
     CDS             102..914
                     /locus_tag="VFC15_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11672.1"
                     /translation="MSTPFQVVVCASVAPDPLQTLEPITGPTGPALKNEMMLPAILDP
                     WAAHALYEGANLVKKVAGSALWLVSMAPKAKLQQVMMTVAQKVPLQLVAVDGPSSGFA
                     DAQQTATALATAIRNIPGIDLTKVLLFGGWESASRSAGVTLQLVGEELGIVDQFQGVD
                     AITPKDDGSFEVLERVEGARHQVSLCAGAPAVLGWATGSLGEPQNNPQVGMANMRTIM
                     PGLQKAVAAKITSDGLQYSNVATPKQQRETRIVKDVPADDIAKEIVQWIGRE"
     gene            923..1954
                     /locus_tag="VFC15_15810"
     CDS             923..1954
                     /locus_tag="VFC15_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11673.1"
                     /translation="MDNILFLAHTEADGSLNKAALEALSSAVELKQSLGGTLDVALYG
                     AQIQPAADSIAACGATRFLAVCGEPFAEARFATDAAASEALLRASGATIVIAAATSRS
                     SRVMAAVAHRVGGCADTHVNAVAAASGATTVTRWFYRQRIEGSLTRSQRPWILLLDPG
                     SRPAWKGEAGSVTPELIDVPLSDSLRRTTVQGVVTPPADQQTIRPDADLLFVAGAGWT
                     KKQKDGQTHVPEAEIIIVDFLRSSKASLGGSKSLVDISGENQSTLHFMTHLNQVGQTG
                     SSPRHPKGLAACCHGEEPHTVGWRFISERRAINLDPNCGWARGKADVLYVADAFEVMT
                     KVNALLSGK"
     gene            2273..2578
                     /locus_tag="VFC15_15815"
     CDS             2273..2578
                     /locus_tag="VFC15_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11674.1"
                     /translation="MVAQCANPACNREFRELSKGRLFLLPPDRHAYAVAMGKVERLSD
                     YCYWLCPECDATHTITRHELEVLVAVRGPGCPYPIAATSDCSKRPTQIAMRNCTQTG"
     gene            complement(2835..3209)
                     /locus_tag="VFC15_15820"
     CDS             complement(2835..3209)
                     /locus_tag="VFC15_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11675.1"
                     /translation="MKKFMKLLLGTSLFLIDQSDRAKKQARGRLGDQLDDLRDLAQQK
                     YEAAADRVARATKALRKNNQNHAIQKTLRFAAGVGVGVGVALLTAPATGQDTRAALAE
                     RAQKIGGNVRKRLASCDLAAGD"
     gene            complement(3260..3412)
                     /locus_tag="VFC15_15825"
     CDS             complement(3260..3412)
                     /locus_tag="VFC15_15825"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZM11676.1"
                     /translation="MPWTVFVILLVLWLLGVVTSYTLGGFIHILLLLALITLIFNLVS
                     GRRTVV"
     gene            complement(3691..4353)
                     /locus_tag="VFC15_15830"
     CDS             complement(3691..4353)
                     /locus_tag="VFC15_15830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11677.1"
                     /translation="MIKKLTLTLGGLVLLASFTGIARADAITFSFVSSATAVHADATG
                     LTAGPARVLLVSDTKIPTDYSLLGNASISTGPASSYSATSNFVSAQYLPGPGIEVKVN
                     SASCVGGTLPGVCLEGIQNSNGEYVGTKNGTGSFQGLFTVTYVSPYITSLFGEPNSWL
                     PAGSDSLNTSANKFKNLGKTDTASLSGGAITFETVPEPGTLGMLGTGLLGLAGVLRRK
                     LQ"
     gene            complement(4599..4913)
                     /locus_tag="VFC15_15835"
     CDS             complement(4599..4913)
                     /locus_tag="VFC15_15835"
                     /inference="COORDINATES: protein motif:HMM:NF037969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZM11678.1"
                     /translation="MYFLKIGSPAHLRWLRVFEPLMVITGIVQPLATLPSISKLYFTH
                     SQHASGQSLTTWSIFALASLLWVIYGLLNRKAAIYAGNIIGLAMNLLMVNGILMHAGW
                     TY"
     gene            complement(4990..7704)
                     /locus_tag="VFC15_15840"
     CDS             complement(4990..7704)
                     /locus_tag="VFC15_15840"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11679.1"
                     /translation="MPQNPESARLEEARTVKTPWRKWGPYLSERQWGTVREDYSQDGN
                     AWDYFTHDQARSRAYHWGEDGIAGISDDQQLLCCSVALWNGKDPILKERMFGLTNSEA
                     NHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAAYPYENLIETNRRRTRNDMEYELLDTG
                     VFNEDRYFDVFVEYAKESPEDLLIQISVSNRGPEAATLHVLPTLWFRNTWTWWAGTPK
                     PSLKQVSAQNGDQVVAAAHAELGERYLYCEGNVPLLFTENETNNERIFGTPNSSPYVK
                     DGINNCVIDGNHNVINPAKTGTKSAAHYQLNVGAGQTATIRLRLSDLAPDALGSPFKT
                     FAATMQTRHAEADEFYKSITPERVGEDEALVMRQALAGMLWSKQFFFFDVDKWLVEHG
                     VDPIKPGGRFMRNREWFHMINQHVISMPDKWEYPWYAAWDLAFHTIALSAVDTDFAKE
                     QLDLMLQEFFLHPTGQIPAYEWNFSDVNPPVHAWASIFLYRTEQALHGKGDIEFLKRS
                     FAKLMLNFSWWVNRKDRFGKNLFEGGFLGLDNIGVFDRSAPLPTGGSLEQADGTAWVS
                     MFAQNMLEIAVELAAHDAFYEDMAMKFAEHFLWIAVAMNQMGPDGMWDEEDGFYYDVL
                     RLPDGSARRLKVRSMVGLLPLAATTVIEPWQRERLPRVMKSLRDRLQRMPEIRNSIHA
                     TGIGHLGYAERGMAALVSEDRLRRILTRMLDENEFLSPYGIRALSRYHQDHPYIVNVE
                     GQEYRVSYLPAESDTGMFGGNSNWRGPIWMPVNAVLIRALLQFYLYYGDNFKIECPTG
                     SGKLMNLYEVSQEIANRLERIFLRDKSGRRPVFGGSEKFQTDPLWKDNILFYEYFHGD
                     NGAGLGASHQTGWTGIVAKLIELFGRIDAQQLLNAGKAGAFVTGDSRR"
     gene            7868..10000
                     /gene="dcp"
                     /locus_tag="VFC15_15845"
     CDS             7868..10000
                     /gene="dcp"
                     /locus_tag="VFC15_15845"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: protein motif:HMM:NF007624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HZM11680.1"
                     /translation="MHTLIIAAAVVIGVVASPALASGAEFGPSNPFFAPSSLPFQAPP
                     FDKIKDEDYQPAIEAGMAEDLKEINAIANNPAPPTFENTIVAMEKSGQLLQRVLAAFF
                     SVSAANTNPELQKVRSIEAPKLAAHADAIHLDSKLFQRVAAVYKERASLKLDPESLRL
                     VEYTYDQFVHAGANLSDADKTELKKLNEELATLSNTFTTKLLAATKDGAYVTTDKAAL
                     AGLSDGKISGAAEAAKGRKVDGFVLPLQNTTQQPDLVSLSNRTTREAVFTNSWNRAER
                     GDANDTRDTIVRIAQLRAQKGKLLGHPNFAAWKIEDQMAKTPEAALKFMNALVPASTA
                     KAATEAQDIQTVIDEQKGGFKVQPWDWDFYAEQVRKAKYDLDEEQVKPYFELDNVLQN
                     GMFYAANQLYGLTFKERKDLPVYAPTVRVFEVFNADGSSLALFYCDYFKSDNKQGGAW
                     MNSLVGQSKLLGTKPVVANVANFSQPAPGEPALLTADDVRTMFHEFGHALHGMFANTE
                     YPSLSGTATARDFVEFPSQFNEHWATYPTVFQHYAKHYKTGAPMPEELLAKIKKSATF
                     NKGYDTTELLAAAELDMMWHTLPASAPVQKPDAFETEALEKTRLSLSYVPPRYRSSYF
                     SHIWGSGYAAGYYAYLWTKMLDDDAYQWLEDNGGLTRANGDRFRTMVLSRGNTEDLEK
                     MYVTWRGAQPSIGPMLKDLGLVSVGASK"
     gene            10181..10720
                     /locus_tag="VFC15_15850"
     CDS             10181..10720
                     /locus_tag="VFC15_15850"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HZM11681.1"
                     /translation="MAPGELATLGSGKSVVWAIVLIIFGFLAIALPFATSYGVVVIIA
                     WLVIFSGGFQFIHAFQSQGIGSTIWKLLVAILYLIMGIYFLLNPLIGVASFTLALAFF
                     FVAEGVIDLVAYFQSRSAGGSVWILVDGIVTLILGILVWRQWPSSSLWLIGTLVGISM
                     IMTGTTRLMIGLAARRALA"
     gene            complement(10845..11549)
                     /locus_tag="VFC15_15855"
     CDS             complement(10845..11549)
                     /locus_tag="VFC15_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11682.1"
                     /translation="MAPALIVVLGALAVPSAAQGVNVTGRVAYYGDANPRTGVSAVVW
                     LVPAAPQASDQPALKPMDAVLRQKNKAFEPHLLVVTKGSSVQFPNLDPWFHNVFSLFN
                     GKKFDLGLYEAGTSRTVHFDREGVSYIFCNIHPEMSAVVVVVSSPYYAVVARNGEFSI
                     AGVPPGRYGLHVWNENSLPDSLEALTREVELNGTMHSVGTLSVREAADAKSQHKNKYG
                     QDYEPASPNNPVYTQP"
     gene            complement(11582..13090)
                     /locus_tag="VFC15_15860"
     CDS             complement(11582..13090)
                     /locus_tag="VFC15_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11683.1"
                     /translation="MKLCQAPRIAQIIVSALLLTVSSAAQNPVHDDSSVTASLNQLQS
                     EVRELKDLVQQLKQETTASRAEMTRLRQELEAERTGLAANPASGADQIDVRVGHLEDE
                     QQMLSGKVDEQYQTKVESASKYRVRLSGVVLFNLFSNQGTVDNMDVPTLAYRASSLQS
                     SGSFGGTLRQSIFGFEVFGPQLWGAKTSGNVNFDIGGGFPNTSDGVNAGLVRLRTATM
                     RFGWKDTDLVVGQDQLFFSPNSPTSYASLIVPALSYAGNLWAWTPQMRVEHRIALSEK
                     SSFTLQGGILDPLTGEPPYYSYTWYRIPQAGERARQPAYAGRIAYSHPLFGHTFTVGT
                     AGYYSREDYGYGRNVNGYAAMADWSLPLDRWFSLNGSFYRGQAIGGLGGGIGRSVLYS
                     GPLIDPTSSVLPLNSVGGWAQLKYRATSTLEFNAAFGQDNPFASDVRAFADAESYGDP
                     TLTRNQGVFANVIYRPRSDLLFSLEYRRLKTFSIYDYDSTAGQVNLGMGILF"
BASE COUNT         2773 a         3882 c         3809 g         2741 t
ORIGIN      
        1 gttccgcgca ggcgttggtg gactgcactc agccgagaac taatctggaa ctttcgcaac
       61 gaaccaaaac aaggaactga agacaaggaa ccaaatcaaa gatgagcact ccttttcaag
      121 ttgtggtttg cgctagtgtc gcgcccgacc cgttgcagac tctggaaccc atcaccggac
      181 caaccggccc ggcgctgaag aatgaaatga tgctgcctgc catcctcgat ccgtgggcgg
      241 cgcatgcgct gtacgagggt gcgaacctgg tgaagaaagt cgctggcagc gcgctctggc
      301 tggtgagcat ggcgcccaaa gccaaactcc aacaggtcat gatgacggtg gcgcagaaag
      361 ttccgttgca gctggtcgct gtcgatggtc cctccagcgg ttttgctgac gcacagcaga
      421 ccgccaccgc gctggcgacc gcgatccgca acatccctgg catcgacctg accaaagtat
      481 tgttgttcgg cggatgggaa tccgccagcc gcagcgccgg agtcacgttg caactcgtgg
      541 gtgaagaact cggcatcgtt gatcagttcc agggcgtgga cgccatcact cctaaggacg
      601 acggttcgtt cgaggtgctg gagcgcgtcg aaggggcgcg tcaccaggta tcgctttgcg
      661 cgggcgctcc cgccgtactc ggatgggcca ccggcagcct gggcgaaccc cagaacaatc
      721 cccaggttgg catggcgaac atgcggacga tcatgccggg gctccagaaa gccgtcgcag
      781 cgaagattac ctccgacgga ctgcagtact ccaacgtcgc caccccgaaa cagcaacgcg
      841 aaactcgcat cgtgaaagat gtgcccgccg acgacatcgc gaaagaaatc gtgcagtgga
      901 tcgggcggga gtaacggaaa tcatggacaa cattctcttt ctcgcgcaca ccgaagcaga
      961 tgggagtctg aacaaagccg cgttggaggc gctcagttcc gcagtggagt taaagcagtc
     1021 gctgggcggg actctcgacg tcgcactcta cggggcgcag attcagcccg ccgccgattc
     1081 cattgctgcc tgtggtgcaa cgcgcttcct cgcggtctgc ggagagccct tcgcagaagc
     1141 ccgctttgca accgatgccg ctgccagcga agccttgctc cgcgcctcag gcgcgaccat
     1201 tgtgattgcc gctgccacct cgcgcagttc gcgcgtcatg gccgccgtcg cgcaccgcgt
     1261 aggtggatgc gccgacactc atgtcaacgc tgtcgccgcc gcatctggcg ctacgactgt
     1321 gactcgctgg ttctatcgcc agcgcatcga aggatcgctg acccgcagcc agcgtccctg
     1381 gattctgctg ctcgatcccg gctcgcgtcc cgcgtggaaa ggtgaggctg gttccgttac
     1441 gccggaactg atcgacgtcc cactctccga cagtctgcgt cgaaccacgg tccagggcgt
     1501 ggtcacgccg cctgcggacc agcagactat tcgtcccgat gcggatctgc tgtttgtcgc
     1561 aggcgcggga tggacgaaga aacagaaaga tgggcagacg cacgttcccg aagcagaaat
     1621 catcatcgtg gattttctgc ggagctccaa ggcgtcgctg ggtggcagca aatcgctggt
     1681 cgatatcagc ggcgagaatc aatcgacgtt gcatttcatg acgcatctca accaagtcgg
     1741 gcaaaccggc tcgtcgccgc gacatcccaa gggactggcc gcatgctgtc atggggagga
     1801 gccgcacacg gttggttggc gctttattag cgaacggcgc gccatcaatc tcgacccgaa
     1861 ttgcggatgg gcacgcggca aagcggatgt gctctatgtc gccgacgcct tcgaggtgat
     1921 gacaaaggtg aacgcgctgc tcagcgggaa ataacctcgt cgttccgtgc cgtacgagct
     1981 ctgtattctt gctattcctt gtggccctca gcccctgtgg aatctgcttt ccgagtattt
     2041 ccgcagcctg ggaagcagta cttcgcaccg tacccgtcgc aaaaccgttc tcgtggttac
     2101 cttcgtaact cgaatcggcg ctttgtgatg gactctgctt cgcgccggac ctaacaatag
     2161 tgataagccg cattccgcaa caaggatggc gctctcaatc gcacgtcagc gtcgatctct
     2221 atgcctgttt cgcatggtga tgagtgcgat ccagcggccg aggaggttcc ccatggtggc
     2281 ccagtgtgcc aaccccgcat gcaatcgcga attccgcgag ctgagtaagg gccggctttt
     2341 cctgcttcct ccggatcgtc atgcctatgc tgtggcgatg ggcaaagtcg agagactgtc
     2401 cgattactgc tattggcttt gcccggaatg cgatgcaacc cataccatca cgcggcacga
     2461 gttggaagtg ctggtcgccg tgcgaggacc cggctgtccc tatccgatcg ccgcaacctc
     2521 ggattgcagc aaaaggccca cgcagattgc gatgcgaaat tgcacgcaaa ccgggtgatg
     2581 gcgtcaccat tcttccgcgg cacgcaatat aaccggccct gcaaacagtt cgcggggcga
     2641 gccgtggtgg ttgctcactg actcgtcccg cactcaggcc ttaacgtctc aggctactct
     2701 ctcggcccgc tccgcgctcg cttgccgatg acgttgaagg cgatcagcga acgagacgaa
     2761 ggtcaggcca cacaaatgct gtgcaacgcg aaattgaggc cagtgctgtc gttcgaagta
     2821 acgcaagttc aggactaatc acccgcagcg agatcgcatg acgccaagcg cttacggacg
     2881 ttaccgccga ttttctgggc gcgctcggcg agcgcagctc gcgtatcttg acccgtagcc
     2941 ggagcagtca acagggccac tccaacccca actcccacgc cggcagcgaa gcgaagggtt
     3001 ttttgaatag cgtggttctg gttgtttttg cgcaaagcct tggtggcgcg cgccacccga
     3061 tccgctgcgg cttcatattt ctgttgagcc aagtcgcgta gatcgtcgag ctggtcgccc
     3121 agccgtcctc gcgcttgctt cttagcgcga tcggattggt cgataaggaa aagactggta
     3181 cccagtaaca acttcataaa tttcttcact ggttaacctc caattggttg atttgagact
     3241 ctgggtctta ctgcgatgtt cacactacgg tccgacggcc gctcaccagg ttgaaaatca
     3301 gcgtgataag cgccagaagc agcaggatgt gaataaaacc tcccaaggta taggaggtaa
     3361 ccactcccaa taaccacaga accaggagaa tcacaaaaac ggtccaaggc atcgccggct
     3421 cctttcgttt tgctccaatg cctgatcgca tcgaagccgg tgcagcgtcg ctgcacgatg
     3481 aaagacagct tagcgttccc gctttcttcc tactatcggg tcaaacctgt agatgcaaac
     3541 gattgtgtac ttacctgtac ttacctatgg ccaggaagtg cgccatgcga gctctatgcg
     3601 ggccgcctcc atcatttcgt attctgcagc acaatagaaa acgccagagc agtttcgctc
     3661 cggcgttttt gcgttacctc ggtctagatt ttattggagc ttgcggcgaa gaactcccgc
     3721 tagtccgagg aggccggtgc caagcatccc aagtgtgccg ggctcgggta ctgtctcgaa
     3781 cgtaatcgcg ccacccgaaa ggctggcagt gtctgtcttg cccaggttct tgaacttgtt
     3841 ggcgctggtg ttcaaggagt cggagcccgc aggcagccag ctattgggct caccgaacag
     3901 cgaagtgatg tacgggctca cgtaggtcac ggtgaacagg ccctgaaacg aacccgtgcc
     3961 attctttgta cccacgtatt caccgttgct gttctgaatg ccctccaggc agactcccgg
     4021 cagagtgcct ccaacgcagg acgcggaatt cactttgacc tcgatgcctg gccccggcag
     4081 gtactgggcg ctcacgaagt tgctcgtggc ggagtaactg gaggcgggtc ccgtcgatat
     4141 cgaagcattc ccgaggaggc tatagtcggt tggtattttt gtatcactga ccagcaggac
     4201 cctcgcaggc ccagccgtta gccccgtggc atcagcgtgc accgcggtag ccgaagacac
     4261 gaaagaaaag gtgattgcat ccgcccgcgc aatgccggta aacgacgcga gaagcacaag
     4321 gcctcccagt gtgagggtca atttcttgat cattcgttga actctcctag gtatgggcga
     4381 ctgatctgac taactcgacg tcgcagccag agttcaacgg tatgctgacg cagaaatcgt
     4441 aacaagagat acaacagccc accgcatgcc ttctctctcg cggcggagtg aagcggtaca
     4501 tactgctaga gaagatggta cgtgggggtg aaagagacgc acggccatga gaagtgagaa
     4561 gggagaagag attggtatca gttacgtagg gtgaagtttt agtaagtcca tcccgcatgc
     4621 atcaggatgc cattcaccat cagcagattc atcgccagtc caatgatgtt cccggcatag
     4681 atggcggcct tgcgattgag taatccgtag atcacccaca acaaacttgc cagggcaaag
     4741 atcgaccacg tggtcagcga ctgaccggaa gcatgctggc tgtgagtgaa atataacttg
     4801 gaaatcgacg gcagggtggc gagcggctga acgattccgg taatcaccat cagtggttcg
     4861 aacacccgca gccagcgcag atgcgccggt gagcctattt tcagaaaata catggcaata
     4921 acctacttgc tggaccgagt cccggatcga tctgcgtatc accaacggga tgccggcgga
     4981 tcgtcttact tacctcctgg agtcgcccgt cacaaaggcg cctgccttac ctgcgttcag
     5041 aagctgctgc gcatctatac gaccgaacaa ctcgatcagt ttggcaacga tacccgtcca
     5101 tcccgtctga tgactggcac caagacccgc accgttatca ccgtggaagt attcgtagaa
     5161 gaggatgttg tccttccaca gcggatcggt ttggaatttc tctgaaccgc cgaagaccgg
     5221 ccgccggccc gacttgtcac gcaggaagat tcgctccagc cgattggcaa tctcctgaga
     5281 cacctcgtag aggttcatca atttccccga gccggtggga cattcgatct tgaagttgtc
     5341 gccgtaatat agatagaact gcagcaaggc acggatcagc actgcattca ccggcatcca
     5401 aatgggaccg cgccagttcg agttgccacc aaacatgcct gtgtccgatt ctgcgggcag
     5461 atagctcacc cggtattcct ggccctccac attgacgata taggggtggt cttggtggta
     5521 acgggagagg gcccggatcc cgtaagggct gaggaattcg ttctcgtcca gcatgcgggt
     5581 caggattcgc cgcagccggt cttcacttac cagcgcagcc atgccgcgct ccgcataccc
     5641 taaatgaccg attccggttg catggatgct gttgcgtatc tcgggcatac gttgcaggcg
     5701 atcccgcagc gatttcatca ctcgcggcaa ccgttcgcgc tgccatggct cgatcacggt
     5761 ggtagcagcc aagggcagca gtcccaccat ggagcggacc ttgagccgtc tggcgctacc
     5821 gtcgggaagg cgcaggacgt cgtagtagaa tccatcctcc tcgtcccaca ttccgtcagg
     5881 tcccatctgg ttcatggcga ccgcgatcca caggaaatgc tccgcaaact tcatagccat
     5941 gtcttcgtag aaagcgtcgt gggccgccaa ctccactgct atctccaaca tgttttgcgc
     6001 gaacatggaa acccatgcgg tgccgtctgc ctgctcgagg ctgccaccgg tgggcagtgg
     6061 ggcgctgcgg tcgaagacgc cgatgttgtc cagtcccagg aagccgccct caaacaggtt
     6121 cttgccaaat cggtctttgc ggttcaccca ccagctaaag ttcagcatca gcttggcgaa
     6181 cgaccgcttc agaaactcga tgtcaccttt accgtgcaaa gcttgttctg tccggtacaa
     6241 aaagatcgag gcccaggcgt gcaccggcgg attgacgtca ctgaagttcc attcgtaggc
     6301 agggatttga ccggtcggat gcaggaagaa ttcctgcagc atcaggtcga gctgttcttt
     6361 ggcgaagtcc gtgtcaaccg cggacagagc gatggtgtgg aaagccaaat cccacgccgc
     6421 ataccagggg tactcccact tgtcgggcat cgagatgacg tgctgattga tcatgtggaa
     6481 ccattcgcga ttcctcatga agcgtccacc gggctttatc ggatcgacac cgtgctccac
     6541 cagccatttg tccacgtcga agaagaaaaa ctgcttggac cagagcatcc cggccaacgc
     6601 ctgccgcata accagtgcct catcttcgcc gacccgttcc ggagtgatgg atttgtagaa
     6661 ctcgtccgcc tcagcatgac gggtctgcat ggtcgccgca aacgttttga acggactccc
     6721 cagggcgtca ggagccagat cgcttagccg cagccggatg gtggccgtct gtccagcgcc
     6781 cacgtttaac tgatagtggg ccgcagattt ggttcccgtc ttcgcagggt tgattacgtt
     6841 gtgatttccg tcaatcacgc aattattgat tccgtctttg acgtaaggag aagagttggg
     6901 ggtaccgaag atgcgctcgt tgttggtctc gttctcggtg aataacagcg ggacgtttcc
     6961 ctcgcagtac agataccgct cgcccaattc ggcgtgtgct gccgcaacca cttgatcgcc
     7021 attctgcgct gagacctgct tcaatgaagg tttgggcgta cccgcccacc acgtccaggt
     7081 gttgcggaac cacagcgtcg gaagcacatg taacgtggct gcctcaggcc cgcgattact
     7141 gacgctgatc tggatgagga gatcttctgg agattctttg gcatattcga cgaaaacgtc
     7201 gaagtagcgg tcctcgttga agacaccggt atccagcagc tcatattcca tgtcgttgcg
     7261 cgtgcgccgc cggttcgtct cgataagatt ttcgtaggga tatgccgcct gcgggtattt
     7321 gtacagatac ttcatgtacg agtgcgtggg cgtgctgtcg aggtagaagt agtattcctt
     7381 aacgtcttcg ccgtggttgg cctcgctgtt cgtcaggcca aacatgcgtt ctttcaggat
     7441 gggatctttg ccattccaca gggccacgct gcaacacaac aactgctggt cgtcgctgat
     7501 gccggcaata ccgtcctcgc cccagtgata cgcgcgggac cgcgcctggt catgcgtgaa
     7561 gtagtcccag gcattgccgt cctggctgta gtcctcgcga actgttcccc actgccgctc
     7621 gctcaggtag ggcccccact ttctccaagg ggttttgact gtacgggctt cctcgagcct
     7681 ggcgctttcc ggattctgcg gcatctttct ctccttggtc gcgtcctcga acaacgcaag
     7741 cccaaatttt acttcaactg catggcgatc gtggattggg agctgccgtc acatcggacg
     7801 ggtgcgctgt gacgaacgcg cccctcagag atgctcgttt tgaccttcac cgggtaaact
     7861 attgaccatg catactctca tcatcgctgc tgctgtcgtt atcggcgtag tcgcatctcc
     7921 tgctctagcg tccggtgctg agtttggtcc cagcaaccct ttctttgcgc ccagctccct
     7981 gccatttcag gcgccgcctt tcgacaagat caaagacgag gactaccagc cggcgattga
     8041 agccgggatg gcggaggacc tcaaggaaat taacgcgatt gcgaataatc ctgcgccgcc
     8101 cacattcgaa aacaccatcg tggcgatgga gaagagtggc cagttgttgc agcgggtgct
     8161 tgcggctttc ttttcggtca gcgccgccaa caccaatcct gaactgcaga aagtcaggtc
     8221 gatcgaggcg cctaaacttg cggcgcatgc ggacgcgatc cacctggatt cgaagttgtt
     8281 tcagcgcgtg gcggcggtct acaaggagcg cgcctcgctc aagctcgatc cggaatcgtt
     8341 gcggctggtt gaatatacct acgatcaatt cgtgcacgcg ggagccaacc tcagcgacgc
     8401 cgataagacc gaactcaaga agctcaacga ggaattagcc actctctcca acaccttcac
     8461 cacgaagttg ctcgcggcca ccaaagacgg cgcctatgtg acgaccgaca aggccgcgct
     8521 ggccggtctg agcgatggga aaatcagcgg cgcggccgag gcagctaagg gtcgcaaggt
     8581 ggacggcttc gtactgcctt tgcagaacac cacccagcag cccgatctcg tctctctgag
     8641 caaccgcacc acccgcgagg cggttttcac aaactcctgg aaccgcgcgg aacgcggcga
     8701 tgccaacgat acgcgtgaca ccatcgtgcg catcgcgcag cttcgcgccc agaaaggcaa
     8761 gctgctcggg catcccaact tcgccgcctg gaaaattgaa gaccagatgg ccaaaacgcc
     8821 tgaagccgcg ctgaaattca tgaacgcact ggtgccggct tccacagcaa aggccgccac
     8881 cgaagcccag gacatccaga ccgtgattga cgagcaaaag ggcggcttca aggtccaacc
     8941 atgggactgg gatttctacg ccgagcaggt tcgcaaggcc aagtacgatc tcgacgagga
     9001 gcaggtcaag ccctatttcg aactcgacaa tgtgctccag aatggaatgt tctacgcggc
     9061 gaaccaattg tatggactga ccttcaaaga gcgcaaagac ctccccgtgt acgcgccgac
     9121 cgtgagagtg ttcgaggtgt ttaacgccga cggcagttcc ctggcgctct tttattgcga
     9181 ctacttcaag agcgacaaca agcagggcgg cgcctggatg aacagccttg tagggcaatc
     9241 aaaactgctc ggcaccaaac ccgtcgtggc caacgtggcc aatttctcac agcccgctcc
     9301 gggcgaaccc gcgctcctca ccgcggacga tgtccgcacc atgttccacg aatttggaca
     9361 cgccctgcac ggcatgttcg cgaacaccga atatccaagc ctgtccggga ccgccacggc
     9421 gcgcgacttc gtcgagtttc cctcgcagtt caatgaacat tgggccacct acccgacggt
     9481 attccagcac tacgcaaagc attacaagac cggagcgccc atgccggagg aactgttggc
     9541 gaagatcaag aagtcggcta ccttcaacaa gggctacgac accaccgagt tgctggctgc
     9601 ggcagagctg gacatgatgt ggcacacgct tccggccagc gcacccgtac agaagccgga
     9661 tgccttcgaa actgaagcgt tggaaaaaac tcgcctctcg ctcagctatg taccgccgcg
     9721 ctaccgttcc agctacttca gccacatctg gggcagcgga tatgcggccg gctactatgc
     9781 ctacctttgg acgaagatgc tcgacgacga tgcataccaa tggttggaag ataacggcgg
     9841 cctgactcgc gcaaacggcg accgcttccg caccatggtg ttatcgcgtg gcaataccga
     9901 ggacctcgag aagatgtacg ttacctggcg cggggcacag cccagcattg gaccgatgtt
     9961 gaaggacctt ggactagtgt cggtcggcgc ttccaaataa atccgccggc aaaagggggc
    10021 ggcgcggtgg ataaactcca ccgcgcgctt ggagtttaga cggcgtcgca tatactgatc
    10081 catcattgac cagtgttgtt cgtagtcacg ataggtatga tggcgtctgg tgggtagctc
    10141 gtagaatttt ccgtagtctg tggaaaggag cggttcaaaa atggcaccag gagaacttgc
    10201 aactctgggc tcaggaaagt cagtcgtatg ggcaattgtg ctcatcatct ttgggttcct
    10261 ggcaatcgcg cttcccttcg caacgtccta tggcgttgtc gtgatcatcg cgtggctcgt
    10321 cattttcagc ggtggttttc agtttatcca cgccttccaa tcgcagggga ttggaagcac
    10381 catctggaag ctgctggtgg cgattctcta cttgatcatg ggaatctact tcctgctgaa
    10441 tccgttgatt ggcgtcgcca gcttcaccct ggcgctggcg ttcttcttcg tggcagaagg
    10501 cgtgatcgac ttggttgcgt atttccaaag tcgtagcgct ggcggctcgg tttggatcct
    10561 tgtggacgga atcgtcactc tgatcctggg catcctggtc tggagacagt ggccctcgag
    10621 ctcactgtgg ctcatcggca cactcgtcgg catcagcatg atcatgaccg gcaccacgcg
    10681 cctgatgatc ggcttggccg cgcgacgcgc tctcgcctga gtaaggaagt tctccgcccg
    10741 caacctcaaa tgagcgggcg gagaattttt tgccgccctc aatccgcagg cagtttttcg
    10801 tcccaatgac aggtgtcgca tttgctgcaa aattggtgcg ctagctacgg ttgggtatag
    10861 acgggattgt ttggcgaggc tggttcgtag tcctggccgt atttgttctt gtgctgggat
    10921 ttggcgtccg ccgcctcgcg gacgctcagc gttcccaccg agtgcatggt cccgttgagt
    10981 tcgacctcac gcgttaacgc ctcaaggctg tcgggcaacg agttctcgtt ccacacgtgg
    11041 agcccgtaac gccccggcgg cacaccagca atggaaaatt cgccattcct cgccaccacc
    11101 gcgtagtacg gactcgaaac caccaccacc accgcgctca tttcgggatg gatgttgcag
    11161 aagatatagg aaacgccctc acgatcgaaa tggacggtcc gcgaagtacc cgcctcgtag
    11221 agtcccaagt cgaatttctt gccgttgaac agcgagaaaa cattgtgaaa ccagggatcg
    11281 aggttgggaa actgcacgga tgagcctttt gtgacgacca gcaaatgagg ttcgaaggcc
    11341 ttgtttttct gacgcaggac ggcatccatc ggcttgagtg caggctgatc gctggcctgc
    11401 ggcgcggcag gcacaagcca caccacggcc gagacaccag ttcgcggatt tgcgtcgccg
    11461 tagtaagcga cgcggccagt aacgttcacg ccctgcgcgg ccgagggcac cgccagtgct
    11521 ccgagcacga caatcaaggc tggcgccaat gcgaactttc ggaattgagg caaagctctc
    11581 cctagaacaa gattcccatc cccaggttga cctgcccggc ggtggaatcg tagtcgtaaa
    11641 tcgaaaacgt cttcaggcga cgatactcca gcgagaagag taagtcagac cgcgggcggt
    11701 aaatcacgtt ggcgaacacg ccctggttgc gagtgagcgt gggatccccg tagctttcgg
    11761 cgtcagcaaa ggcacgcaca tccgaggcga acgggttgtc ttgcccaaag gcggcgttga
    11821 actcaagggt ggacgtggcg cggtatttca gttgcgccca tccgcctacg ctattgagcg
    11881 gaagcacgct gctggtggga tcgatcagcg gcccactgta cagcacgctg cgtccgatgc
    11941 cgccgccaag cccaccaatc gcctggcccc ggtagaacga tccgttcagc gagaaccagc
    12001 ggtcgagagg aaggctccaa tcggccatgg cggcgtagcc gttcacgttg cgtccgtatc
    12061 cgtagtcttc gcggctatag tagccggcag tcccgacggt gaaggtgtgg ccgaacagcg
    12121 gatgcgaata ggcgatgcgt ccagcatatg ccggttgccg ggcgcgctcg ccggcctgcg
    12181 gaatgcgata ccaggtgtag gaataataag gtggctcgcc ggtcagcggg tcgagaatgc
    12241 cgccctgcag cgtgaaggag gacttctccg atagggcgat gcgatgttcg acgcgcatct
    12301 gcggggtcca tgcccacaag ttgccggcat acgacagagc gggcacgatc aacgaggcgt
    12361 aagaagtcgg ggagttgggc gaaaagaaga gctggtcctg tccgacgacg aggtcggtgt
    12421 ccttccaacc gaagcgcatg gtggcggtgc gcaggcggac caagccggcg ttcactccgt
    12481 cggaagtgtt gggaaagcct ccgccgatgt cgaagttcac gttgccgctg gtcttggctc
    12541 cccatagctg aggcccgaac acctcgaagc cgaagatcga ctgccgcagc gtgccgccga
    12601 aagagcctga cgattgcagg ctactggcgc gataggccag cgtggggacg tccatgttgt
    12661 caaccgtgcc ctgattgcta aacaaattga agagcaccac tcctgacagc ctcacgcgat
    12721 actttgacgc gctctcgacc tttgtctgat attgctcatc gacttttccc gagagcattt
    12781 gctgctcgtc ctcaaggtgg ccgacgcgca cgtcgatctg gtcggcgccg ctcgcggggt
    12841 tggccgcgag gccagtgcgc tccgcctcca gttcctgccg aaggcgcgtc atctcggcgc
    12901 gtgaggcggt ggtctcctgc ttgagctgct gtaccaggtc cttcaattcg cggacctcag
    12961 actgcagttg gttcaacgac gccgtaacgc tgctgtcgtc gtgcacagga ttctgggcag
    13021 ccgagctgac agtgagcaac aaggcgctga cgatgatttg cgctatgcgc ggtgcctggc
    13081 agagtttcat ttgccacctc tgtgaactga atcgatgtgg tccctggccg atagcctaga
    13141 tctccatgct gccggtgcgt gcggacgctg cggtgactgc ccccatgcac ggcagggtga
    13201 tacgc
//
LOCUS       DASRKK010000649         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_62884, whole genome shotgun sequence.
ACCESSION   DASRKK010000649 DASRKK010000000
VERSION     DASRKK010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62884"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFC15_15865"
     CDS             <1..1034
                     /locus_tag="VFC15_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11684.1"
                     /translation="FLAVDDVAALQAAADRLSPAIIRKQLDYWTLILGPKFSKKERGQ
                     MNLSRFYAIAQIEYCRNFIFKRHFPIHKIFERSCEIGLWRLTANRISEIFGVRLHKRL
                     RGKLATVIDQIEHGHHVFRAYWKNAFLKQYEKFSRYLRNELCSNNLRDFGLKKGLDHL
                     DAVRNRFQAITDRFAGFQAECLNVHVDFPLLQRLALPITIGSVRYPGIKIHDARIIRL
                     LEVLLHGGNTVGGWTAKQIHEAVLTTFQLSAKTYGLNQLRYDLRKLKGHGLLERDGSR
                     YAYQLTAKGVQVALLFLFFHKRLCGPLANSRFHHQPDPAHRPNSKLEAAYHKPDKAIQ
                     NIVDLLAAA"
     gene            complement(1374..1694)
                     /locus_tag="VFC15_15870"
     CDS             complement(1374..1694)
                     /locus_tag="VFC15_15870"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM11685.1"
                     /translation="MSQKRIQQIEQRIDRSKRALLEIGPMRPGSLTRQYKDPKNQTGA
                     YWQISYTRQMKSRTEYVRQECVKELRHRIATHKRFKRLVDQWIDLSIEHSRLTMQMAE
                     SKAD"
     gene            1803..3209
                     /locus_tag="VFC15_15875"
     CDS             1803..3209
                     /locus_tag="VFC15_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11686.1"
                     /translation="MLWNHWWNLVCDLQPACARTRTFLWMSLCLAGMTVRKDLLGVTS
                     IVRALGLEPACYDRLLDFFHSPALDLDKLTRAWCTLLFRLDPGILRVDGRPVLVGDGI
                     KVAKAGRKMPGVKKLHQESESNNKAEYIFGHSCQAVAVLTQALSSVFALPLACRIHEG
                     TVFSNRDHRTLLDKMILLLGSLGLNQPFYFVADAYYASGKVVRGLLAHGNHLVTRVRC
                     NSVAFFPATPPSPHRLPRKGRPAKYGKKLKIATLLKDVGQLQEAPSPVYGERGLTLRF
                     RSADLLWRPVGILVRFVVVLHPQRGAILLMGTDLTLPALDIIRIYGLRFKIEVSFKQA
                     IHVIGAYAYHFWMAAMTPIRRVSGNQYLHHKSDDYRNAVRRKIAAYHRHIQLGLIAQG
                     LLQILSATQPRLVWRSFGSCIRTIRPGLAPSEQVAAVALRNTLPDFLATAAKTSILVK
                     FIRDRLDLSRTEGTSLAA"
BASE COUNT          657 a         1121 c          890 g          710 t
ORIGIN      
        1 ctttcctggc ggtggacgac gtggccgcct tacaggccgc cgccgaccgg ctgagccccg
       61 ccatcatccg caagcaactc gattattgga ccctcatcct ggggccgaag ttctccaaaa
      121 aggaacgcgg ccagatgaac ctgtcgcgct tctacgccat cgctcagatc gagtactgcc
      181 ggaacttcat cttcaaacgc cacttcccca ttcataagat cttcgagcga agctgcgaga
      241 tcggcctctg gcggttgacc gccaaccgga tcagcgagat cttcggtgtg cgcttgcaca
      301 agcgcctgcg cggcaaactc gccaccgtga tcgaccagat cgagcatggc catcatgttt
      361 tccgcgccta ctggaagaac gcctttctca aacagtatga gaagttctcc cgctatctgc
      421 gcaacgaact ctgctctaac aatctgcgcg acttcgggct gaagaaggga ctcgaccatc
      481 tcgacgctgt gcggaacaga ttccaggcga tcaccgatcg ctttgccggc ttccaggcgg
      541 agtgcctcaa cgtccatgtg gacttccccc tactccaacg actggcccta cccatcacca
      601 tcggttcggt gcggtatccc ggcatcaaga tccatgacgc ccggatcatc cggttgctgg
      661 aggtgctctt acacggcggc aataccgtcg gcggctggac cgccaagcag atccatgaag
      721 ccgttctcac cactttccaa ctttcggcga aaacctacgg cttaaatcag ctccgctatg
      781 acctgcgcaa actcaagggg cacggcctgc tggaacggga tggctcccgg tacgcttacc
      841 agctcactgc caaaggcgtc caggtcgcac tcttgttcct gttctttcac aagcgcctgt
      901 gtggacccct ggccaacagc cggtttcacc accaacccga ccctgcgcac cgccccaaca
      961 gcaaactcga agcggcctac cacaagcccg acaaggccat ccaaaacatc gtcgatctgc
     1021 tggctgccgc gtgaagttat cgcgtcaact gttgaagtat tcttgtttac aatcttcgat
     1081 ctaagaatct aacactggcc agaatgggct gacaacggga tccaggcttc gcggctatgg
     1141 gttttgaatc tcgtggagtt ggatgaggac ggacgaaaag cgggctcacg gcagggttgg
     1201 aggtaggaaa gttgggtaac ctcgccgaca gaaggtccga gtcggtgtat ggccgagttg
     1261 cgagctgcgg gtgcaccata ttcatggact ggccgcccat attagaatgg gagccatgca
     1321 cgtgcatagt tgggttgcgt acaaacaact tgagagtacc gaattccccc caatcagtca
     1381 gccttcgatt ccgccatctg catggtcagc cgggagtgct cgatactcag gtcgatccac
     1441 tggtcaacca ggcgtttgaa ccgcttgtgg gttgcgatcc ggtggcgaag ctccttcacg
     1501 cattcctggc gaacgtactc cgtgcggctc ttcatctgac gggtatagct gatctgccag
     1561 taggccccgg tttggttttt ggggtccttg tactggcggg tgagcgagcc cgggcgcatc
     1621 gggccgatct ccagtagcgc cctcttgctc cgatcaatcc tctgctcgat ctgctgaatc
     1681 cgtttctggc tcatgagtcc tcctgttgcg tcgttagtat agcatatata tgctatactg
     1741 atccagtcaa ttatcgacac cgacaacccg aagttcgagc cgaccggcaa ggaggccgct
     1801 tcatgctctg gaaccattgg tggaatctgg tgtgcgattt gcagcccgcc tgcgcccgca
     1861 cccggacctt cctctggatg tccctctgtc tggcgggaat gaccgttcga aaggatctgc
     1921 tgggcgtcac cagcatcgtc cgcgccctgg gcctggagcc ggcctgttac gaccgcctcc
     1981 tggacttctt ccacagcccg gccctggatc tagacaagct cacccgcgcc tggtgcactc
     2041 tgctcttccg gctcgacccc ggcatcctgc gcgtcgacgg caggccggtt ctggttggcg
     2101 acggaatcaa ggttgccaag gccggccgca agatgcctgg agtcaagaaa ctccatcagg
     2161 aatccgagtc caataacaag gccgagtaca tcttcggcca ctcctgccag gcggtcgctg
     2221 tgctcaccca agccctgtcc agcgtttttg ccttgccttt ggcctgtcgc atccacgaag
     2281 gcacggtgtt ttccaaccgc gaccatcgaa ccctgctgga caagatgatt ctgctgctcg
     2341 gctcactggg gctgaatcag ccgttctact tcgtggccga cgcttactat gccagcggca
     2401 aagtagtgcg cgggctgctg gcccatggaa accacttggt cacgcgcgtc agatgcaaca
     2461 gcgtagcttt cttcccggcc acacccccgt cgccacatcg gctgccgcgt aagggtagac
     2521 ccgccaagta cggcaagaag ctcaagatcg cgactctgct gaaagatgtc ggccagctcc
     2581 aggaggcgcc cagccccgtc tatggcgaac gagggctgac cctacgcttc cgctccgccg
     2641 acctgttgtg gcgccccgtc ggtattctcg tccgcttcgt cgtggtgctc catcctcagc
     2701 gcggcgccat cctgctgatg ggcacggacc tgaccctccc cgctttggac atcatccgca
     2761 tctatggcct gcgtttcaag atcgaagtgt ccttcaagca ggccatccac gtcatcggcg
     2821 cctatgccta tcacttctgg atggcggcca tgacgcccat tcggcgcgtc agcggcaacc
     2881 agtacctgca ccacaaatcg gatgactacc gcaacgccgt ccgccgaaag atcgcagcct
     2941 accaccgcca catccaactc ggactcatcg cccaggggct gcttcagatc ctctccgcca
     3001 cgcaacccag gctggtctgg cgatccttcg gctcctgcat ccgcaccatc cgcccgggac
     3061 tggccccctc cgaacaggtc gccgccgtcg ccctccgcaa taccctcccg gactttctag
     3121 caactgccgc caaaacctca atcctcgtga aattcatccg cgaccggctt gacctgtccc
     3181 gaaccgaagg aaccagcttg gcggcatgag ccgcccaccc cctgatttcg gtactctcaa
     3241 gttcaacagt ctgctcgtct ttatctatca ctgcttcgac cgcatcgtca tccacggcta
     3301 cctcagcggt ctgtccagac ccgaacaagt ggtctacttt ttccacgagg tcctcggcac
     3361 cccggtggtg agcaagga
//
LOCUS       DASRKK010000650         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_256660, whole genome shotgun sequence.
ACCESSION   DASRKK010000650 DASRKK010000000
VERSION     DASRKK010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256660"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFC15_15880"
     CDS             complement(<1..264)
                     /locus_tag="VFC15_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11687.1"
                     /translation="MPANAVETASASSTADFKSLEEKILRTIELLKSAREAKAAAERD
                     ASRLREQLNEREEEIDTMRQEMVGLRREREEVRTRVEKMLDQIE"
     gene            474..1043
                     /locus_tag="VFC15_15885"
     CDS             474..1043
                     /locus_tag="VFC15_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM11688.1"
                     /translation="MKAQYTPDEYVAELQRQTTQAASLVEGLGVALLNWQPMAGKSWS
                     VGQCLDHLTRTNTISLDAMRAAVEENRDQLEPRTKDLQPSGPVTRFYVKFSEPPPKMK
                     IPAPKKIAPPSQLSEDVLTKFAAMQNCLVEFLRQYGGADLGDVKYKNSLVTNFRLTID
                     TGLVILAVHNRRHLWQAEQVRKNPSFPRG"
     gene            complement(1051..>2203)
                     /locus_tag="VFC15_15890"
     CDS             complement(1051..>2203)
                     /locus_tag="VFC15_15890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZM11689.1"
                     /translation="RSEVNRILGIDLDEDEIQRILVRLGFGVTRAPAGDFSVQIPAWR
                     LDVEREVDLLEELARIHGYNKFPNTLPAFVGAVVALPDEPKNAKVRETMLALGYNEGI
                     SITFISEQEAKAFGAGEPLAIANPLSEEAGYLRTSLIPGVLNMVGYNLNRGNANVRLF
                     EAGEVFEKLGDRHDERRHLGFVATGDAIAKSVHSPAQPYTFFHMKGDVEELLASFEHQ
                     KLEFDDQIPQYFHPGRSARAVMDGETLAYFGQLHPDLAAARKLRQEVYIAELMLDRLY
                     RHPLREPRYRRISKFPAVERDFSFIFDEAITFERIRSAIEDLGLGELQKFVPAEIFRG
                     EKVGPGKYSVLLHAEFQSKERTLRDDEVAQWSEKIVAKLQSLGGVLRAQ"
BASE COUNT          463 a          657 c          654 g          429 t
ORIGIN      
        1 ctcaatttgg tctagcatct tttctactcg ggtacgtacc tcttcgcgct cgcggcgcag
       61 gccgaccatt tcctggcgca tggtgtcgat ctcctcttcg cgctcattca actgctcgcg
      121 caggcgggag gcatcgcgct cagcggcggc cttggcctcg cgggcggact tcaacagttc
      181 gatggtgcgc agaatctttt cttccagcga cttgaaatcg gcggtagaag aagcggaggc
      241 ggtttcgact gcgttcgcgg gcatataaag aatgacctca aaaaaatttg atgtcgcgag
      301 cgcagtatag cgcagaaatt agctcgcgat tcgaaagtgg ccgacgattc cgcgaaattc
      361 agcggcggcg aacctgaaga tgcaactgcg gcggtctcca ggagcgtttg gggcgatgcc
      421 gagtagatgg cgcgtagaat cgagcgtcgc tcaagggaag gaagaaccaa gccatgaagg
      481 cgcaatacac gcccgacgag tacgtggcgg aactacagcg gcagacaacc caggcagcgt
      541 cgctggtaga gggtctagga gttgctttgc tgaactggca gccgatggcc ggaaaatcct
      601 ggagcgtggg ccagtgcctc gaccacctca cccgcaccaa caccatctct ctggatgcca
      661 tgcgcgccgc cgtggaagag aatcgcgacc aactggagcc tcgcacaaag gacctccagc
      721 catcgggccc cgtgacgcgt ttctacgtaa agttctcgga accgcctccg aagatgaaga
      781 ttcccgcacc caaaaagatc gctccgcctt cgcaactctc ggaggacgtg cttaccaagt
      841 tcgcggcgat gcagaattgc cttgtggagt tcttgcggca gtacggcgga gctgacttgg
      901 gcgatgtgaa gtacaaaaac tcgctggtaa cgaatttccg tctcaccatc gataccgggc
      961 tggtgatcct ggccgtccac aatcgacgcc atttgtggca ggcagagcag gtaagaaaga
     1021 atccaagttt cccgcgcggt tgatctttag ttattgcgcg cgcagcactc cgcccagcga
     1081 ttgcagtttc gcgacgatct tctccgacca ttgcgccact tcgtcgtcgc gtaatgtgcg
     1141 ctccttcgac tggaactccg cgtggaggag caccgaatac ttgccagggc caaccttttc
     1201 gccgcgaaag atctctgccg ggacgaactt ctgtagctcg ccaagtccca agtcttcgat
     1261 ggccgaccgg atgcgctcga aggtgatcgc ttcatcgaag atgaacgaga aatcacgctc
     1321 gaccgccggg aacttcgaga ttcgccgata acgcggctcg cgcaagggat ggcgatacag
     1381 gcgatcgagc atcaactctg cgatgtaaac ctcctggcgg agcttgcgcg cagccgccag
     1441 gtcaggatgc agttgcccaa agtaggcgag ggtctcgccg tccatcaccg cgcgcgccga
     1501 gcggccggga tggaagtact gtggtatctg atcgtcgaat tccagcttct gatgctcgaa
     1561 ggacgcaagc agttcttcga cgtcgccctt catgtggaag aacgtatacg gctgcgccgg
     1621 cgagtggacg ctcttcgcga tggcgtcacc ggtagcgaca aaacccaagt gtcggcgctc
     1681 atcgtgccgg tcgcccagct tctcgaagac ctcgcctgcc tcgaacagcc gcacgttagc
     1741 gttgccccga ttcaggttgt aacccaccat gttcagcacg ccgggaatga gcgacgtgcg
     1801 caggtacccc gcctcttcac tgagcggatt ggcgatggcc agcggctcgc ctgcgccgaa
     1861 ggccttggcc tcctgctcgg agatgaaggt gatcgaaatg ccctcgttgt agccgagcgc
     1921 gagcatggtc tcgcggacct tggcattctt cggctcgtcc ggcagcgcga caaccgctcc
     1981 aacaaacgcg ggcagcgtgt tgggaaactt gttgtaaccg tggatgcgcg cgagctcttc
     2041 cagcaggtca acctcgcgct ccacgtcgag acgccacgcg ggaatttgca cactgaagtc
     2101 gcccgcaggt gcgcgcgtga cgccgaaacc gaggcgaact aggatgcgct ggatctcatc
     2161 ttcatcaaga tcgatgccga gaatacgatt cacctccgag cga
//
LOCUS       DASRKK010000651         6127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1137312, whole genome shotgun sequence.
ACCESSION   DASRKK010000651 DASRKK010000000
VERSION     DASRKK010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137312"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFC15_15895"
     CDS             <1..184
                     /locus_tag="VFC15_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11690.1"
                     /translation="IKKTEVAILATAHNSAEDQDEWVVAIINLKSRILREFKNRERPW
                     FATFSRTAKLTIATIK"
     gene            211..2154
                     /locus_tag="VFC15_15900"
     CDS             211..2154
                     /locus_tag="VFC15_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZM11691.1"
                     /translation="MIQLSSAGKRFGPKLLFNDLDWLITPRDRVGLVGANGTGKSTLL
                     KILGGIESLDYGTVTPAKNMTFGYLPQDGLQLSGRTVFDECMSVFSDLRSLEQEMEEL
                     AHKMGKLDHTSSEYGQVADRYQRITGEFRVRDGYALDSQVGTVLDGLGFRKEDWTRRT
                     EEFSGGWQMRIALAKLLLAKPNLLLLDEPTNHLDLETRNWLEQYLLQYPYAYLLISHD
                     RYFLDVTVDRIVEIWNKRVQFYSGNYEKYLQQKQERRDSLVAAYKNQRERVEQLEAFI
                     NRFRAQATKAKQVQSRIKELEKIDRIEIPPDEKTIHFSFPQPKPSGRLVVECAGVAKS
                     YGEKEVFRNVEFTIQRGDRIALVGVNGAGKSTLIKLLTGIEPLTAGTLQLGHNVEPDY
                     FAQDQYKELDPTRRMLDDLSDVAGQKSMTELRGVLGCFLFSEDDVFKPIGVLSGGERN
                     RYALARMLLHPSNFLLLDEPTNHLDLRAKDVLLESLEKYNGTVVFVSHDRYFIDKLAT
                     RVFEIGSGRVEVFPGNYEDYLWRKQRAGVAEEVTGPSNGGGSWAPTLSDVPGYEEQKP
                     ASRKRVNPIKRKQMEERCKQVEEEIAQLESGIAECERELQSFVSAEETARWTALLAKG
                     RADLGPLMAEWEELSQVLEQANA"
     gene            complement(2162..4123)
                     /locus_tag="VFC15_15905"
     CDS             complement(2162..4123)
                     /locus_tag="VFC15_15905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM11692.1"
                     /translation="MASEIEVVDSQGELHSDAVTQVEDSGADSRFISGPLGENRRQVP
                     IEPPKPPFEDGRQLAAKLGVSFALLIIILLGTAYLTLHRMQQMNASARDTLNESLVEL
                     QLGQEALRYSSENSRITMQVFLVKRQEVIDELLARRAENSRKIATLVSALEPRCKPGE
                     ETRLLQTVKQTREPYIGSYQLALHLLLSEKKRDAAMELMVQQTTPALYRYHAAWDDFL
                     RFQNEQVRVATEQSEQHEAAAQRTMLTIILIVGFLAATIAIIATRWVARVVTSRIRMQ
                     RKVCELNTGLEQRVAQRTQELARADQQLRGSLQELQDYTKEMEAINELVELLQSCLTL
                     DEARQQASRILQQFFPSGAMLMLNASRNLLDVVCRWGSAAVKPGPFAPESCWALRKGC
                     VHLVQPNNFSLVCGHIDEASASCHLCVPIVAQGDSLGVLSIDDPHLCDSIAHPRLLQR
                     KQELATTVAEQISLAFANLTLRETLKYQAVRDPLTGLFNRRHMEESLERELLRAARNA
                     KPVTVMMIDIDHFKKFNDSFGHEAGDLLLRELGSTFSSITRGGDIACRYGGEEFLLIL
                     AEASLEAGYERAVKLKEQVANLQVRYRGETLRRITVSIGVAAFPQHGTSASQIIRLAD
                     QALYQAKAEGRDRVVVAAAISTEHDMSLA"
     gene            complement(4311..5810)
                     /locus_tag="VFC15_15910"
     CDS             complement(4311..5810)
                     /locus_tag="VFC15_15910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM11693.1"
                     /translation="MKSSPYRARFISTSLIALVILAFAISAAAQLAPARVQLAASAVP
                     GQSAPPEKVASPASALCAACVRENLEYLAGPKLHGRGSGTIDEYLAAEFIARKLEQYG
                     VAPAAENGQYIQTASLQSRAVTKAPVLSFDMGDSASPRTIAWTHGKEIAAFRLSEPNV
                     QGPLQKLDLIVATPSPNSVRDSAVVLLKLKPETSRKDFQGMLGPYLHSKAAMVIVPVF
                     PAAKAMFDRLSKEMPKAGVKMGNAPLGPDVIFARPEATERLWALPDGTFIKLHSAMTS
                     WKTSHTWNVLGKIVGTNEKDQVVLLSAHLDHLGVKDGRTYPGADDDASGTVAVMELAH
                     ALVAGPKPSRTVVFALWGSEETGMIGSGYFLKNPTFALKNIVANLEFEMIARPDPRLE
                     RDQLWLSGWERTDLGPELAEHGAKLVGDPHPDQNFFARSDNYPLAREGIVAQTVSSYG
                     LHKDYHQPTDTLAKVDWQHLDSAIGSMIGPVTWLVNSAFAPQWNNGQRP"
     gene            complement(5833..>6127)
                     /locus_tag="VFC15_15915"
     CDS             complement(5833..>6127)
                     /locus_tag="VFC15_15915"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZM11694.1"
                     /translation="TAAVKGSKTRIQWPWFILFFCLAAVANTYLFPGAIAYKFLSNAG
                     KLMLIITLFLIGASLSPAALRKVGPRPLLQGVLLWIVVAVVSLLAIRAGWIGI"
BASE COUNT         1296 a         1804 c         1781 g         1246 t
ORIGIN      
        1 gatcaagaag acagaagtcg caattttggc gactgctcac aacagtgctg aagatcaaga
       61 cgaatgggtt gtagcgatta tcaacctgaa gagccgaata ctccgcgaat tcaagaaccg
      121 tgagagaccc tggttcgcga ccttcagtcg cacagcaaag ctcaccattg cgaccattaa
      181 atagactcac tcgagcgcgt ttcactgacc atgattcaac tctcttccgc cgggaaacgc
      241 ttcggtccca aacttctctt caatgacctc gattggctca tcaccccgcg cgatcgcgtc
      301 gggctggtgg gcgccaatgg cactggcaaa tcaactctgc tcaagatctt gggcgggatc
      361 gagtcgctcg attacggcac cgtcacaccc gcaaagaata tgaccttcgg ctatctgccg
      421 caggatggtc tgcagctctc cggccgcacc gtgttcgacg aatgcatgtc ggtgttcagc
      481 gacctgcgct cgctggaaca ggagatggaa gagctcgccc acaagatggg caaactggac
      541 catacttcca gcgaatacgg gcaagtggct gaccgctacc agcgcattac cggcgagttc
      601 cgcgtgcgtg acggttacgc cctcgattcg caggtcggca ccgtactgga cggccttggc
      661 ttccgcaagg aagactggac gcggcggacg gaagagtttt ccggcggctg gcagatgcgc
      721 atcgcactgg ccaagctgct gctggccaaa cccaacctgc tgctactcga cgaacccacc
      781 aatcatctcg atctcgaaac ccgcaactgg ctggagcaat atctcctgca atatccgtac
      841 gcctatctgc tcatctcgca cgaccgctac ttcctcgacg taacggtgga tcgcatcgtc
      901 gaaatatgga acaaacgcgt gcagttttac agcggaaatt acgagaagta tttgcagcag
      961 aaacaggagc gccgtgactc gctggtagcg gcgtacaaga accagcggga gcgcgtcgag
     1021 cagcttgaag cctttatcaa tcgctttcgc gcgcaggcga ccaaggcgaa gcaggtgcaa
     1081 agccgcatca aggaactcga gaaaatcgac cgcatcgaga ttcccccgga cgagaagacc
     1141 atccacttca gttttccaca acccaaaccc agcggccgat tggtcgtcga atgcgcgggc
     1201 gtggcaaaga gctatggcga aaaggaagtt ttccgcaacg ttgagttcac gatacagcgc
     1261 ggagatcgca ttgcgctggt cggagtgaac ggggcgggca aatcgacgct gatcaagctt
     1321 ctcaccggca ttgagccgct cacggcgggc acgctgcagc ttggtcacaa tgtggagccg
     1381 gattatttcg cgcaggacca atacaaggaa ctcgatccca ctcgccgcat gctcgacgac
     1441 ctttccgatg tagcggggca gaagtcgatg actgaattac gtggcgtgct gggctgcttc
     1501 cttttctccg aagatgacgt gttcaagccg attggggtgc tctcgggcgg cgagcgcaat
     1561 cggtatgcgc tggcccgcat gttgctgcat ccgtcaaact ttctgctgct cgacgagcct
     1621 acaaaccatc tcgacctgcg cgccaaagac gttctgctgg agtcgctgga gaaatataac
     1681 ggcaccgtgg ttttcgtctc gcacgatcgc tacttcatcg acaagctggc tacgcgcgtc
     1741 ttcgaaatcg gcagtggccg ggttgaggtt tttccgggta attacgaaga ctacctctgg
     1801 cgcaagcagc gagctggcgt ggcggaggag gtaaccggtc cgtcgaatgg aggcggtagc
     1861 tgggctccca cgttgtcaga cgtccccggc tatgaggaac agaagccggc atccaggaag
     1921 cgcgtgaacc cgatcaagcg caagcaaatg gaagagcgat gcaagcaagt cgaggaagag
     1981 atcgcgcaac tggagagcgg gatcgccgag tgcgagcgcg agttgcagtc gtttgtaagc
     2041 gcggaagaga cggcgcgctg gaccgccttg ctggcaaaag gtcgcgccga tctgggaccg
     2101 ctgatggcgg agtgggaaga actctcgcaa gtgctggagc aggccaacgc ctagtttggc
     2161 gctaagccag ggacatgtcg tgttctgtgc ttattgcggc ggcaacgacc actcggtcgc
     2221 gcccctcggc tttagcctgg tacagcgctt ggtccgccaa cctgataatc tgtgaagcag
     2281 aggtgccgtg ctgcggaaat gctgccaccc cgattgatac cgtaatccgg cgcagggttt
     2341 ctccgcgata gcggacctgc aggttcgcga cctgctcctt gagcttcacc gcccgttcgt
     2401 aaccagcctc cagactggcc tccgccagga tcagcagaaa ctcctcgccc ccgtaccggc
     2461 aggcaatatc gccccctcgt gttatagagc tgaaggtgga gcctaattct cgcagcagga
     2521 ggtcgcccgc ctcgtggcca aagctgtcat tgaacttctt gaaatgatcg atatcgatca
     2581 tcattacggt caccggtttt gcgtttcggg cggcgcgcag caactcccgt tccagtgact
     2641 cttccatgtg acgacggttg aacaggccag tcagaggatc gcggacggcc tggtatttca
     2701 aagtctcgcg cagcgtaaga ttggcaaaag ccagcgaaat ctgctccgcc acggtagttg
     2761 cgagctcctg cttgcgctgc agcagccgcg gatgggcaat agaatcgcac aggtgcggat
     2821 cgtcgatgct aagcacgccc aaggagtcgc cttgcgcgac tatgggaaca cagagatggc
     2881 aggaggcaga agcctcatca atatgcccac acactaaact aaagttgtta ggttgaacca
     2941 agtgcacgca tcctttgcgc aaagcccagc agctctccgg ggcaaaaggc cctggcttta
     3001 cagcggcgct cccccaacgg cagacaacat caagcagatt gcgcgatgcg ttcagcatca
     3061 acatcgcccc cgaggggaag aactgttgca ggatgcggga agcttgctgg cgcgcctcgt
     3121 ccagagtcag acacgattgc aatagttcca ccagttcgtt gatggcttcc atttctttgg
     3181 tatagtcctg cagctcctgc aaggagccgc gcaattgctg gtcggcgcgc gccagctctt
     3241 gggtacgctg cgccacgcgc tgctccagcc cggtgttaag ctcgcagact ttcctctgca
     3301 tgcgaatccg cgatgtcacc actcgtgcca cccagcgggt ggcaattatg gcgatggtcg
     3361 ctgccagaaa acctacaatc aggataatgg tgagcatagt gcgttgggcg gccgcttcgt
     3421 gctgctcgct ctgctcggtc gccaccctga cttgttcgtt ctggaaccgc aaaaaatcgt
     3481 cccaggctgc gtgatagcgg tacagtgcag gggtggtttg ctggaccatc agctccatcg
     3541 cagcgtcccg ttttttctcg ctcagcagca aatgcagtgc tagctggtaa ctgccaatgt
     3601 agggctctcg agtttgtttt acggtttgca gcagccgcgt ctcctcgccg ggtttacaac
     3661 gaggctcgag ggccgaaacg agagtggcga tcttacggct gttttccgcc cggcgggcca
     3721 gcagttcgtc aatcacctct tgccgcttta ccaggaaaac ctgcatggtg attcggctgt
     3781 tctcactgga gtagcgcagg gcttcttgcc ccagttgcag ttcaaccagg ctttcgttga
     3841 gcgtgtctcg cgcgcttgcg ttcatttgct gcatgcgatg cagcgttaga taagcggtgc
     3901 ccagcaggat gatgatcagc agggcgaatg acacgcccag ctttgccgcg agctgccgcc
     3961 cgtcttcgaa cggtggcttc ggtggctcta tgggtacttg gcgtctgttc tccccgagcg
     4021 ggccggagat aaagcggctg tctgccccac tgtcttcaac ctgagtgacc gcgtcgctgt
     4081 gcaactctcc ctggctgtcc acaacttcga tttcagaagc catccacaat ctccaacctg
     4141 acgagccgtc gcgggggcaa cagacctctt gccccacttt gaggctacag ccccaggggg
     4201 ctaggtgtgg ggttaatttc ctctcaggat gggttacgaa agtacatgaa aggcgagttt
     4261 gggaaatgtc ctgcttcggg aagcatcgtc cgtactaccg agctatccgg ttagggcctc
     4321 tgtccgttat tccactgcgg cgcgaaggcg ctgttgacca gccaagttac agggccaatc
     4381 atcgagccaa tcgcactgtc gaggtgctgc cagtccacct tcgccagcgt gtccgtgggc
     4441 tggtgatagt ccttgtgcaa gccgtagctc gataccgtct gcgccacgat tccctccctc
     4501 gccagtggat aattgtccga acgggcaaaa aagttttgat cgggatgcgg gtcgccaacc
     4561 agcttggccc cgtgttcggc gagttccggc cccaggtcag tgcgctccca tcccgagagc
     4621 catagctggt cgcgttccag ccttggatcg ggacgcgcaa tcatctcgaa ctcgagattc
     4681 gccacgatgt tcttcaatgc aaaagtcggg tttttcagga aataccccga accaatcatg
     4741 cccgtctctt cgctgcccca cagcgcaaat accacggtgc gactgggctt cggccctgcc
     4801 accagcgcgt gcgccagctc catcacggcc acggtgccgg aggcgtcgtc atccgctccg
     4861 ggataggtcc tgccatcctt cacgccgagg tgatcaaggt gagcgctgag caggacgacc
     4921 tggtccttct cgttcgtgcc aacaatcttg cccagcacat tccaagtgtg cgaggttttc
     4981 cacgacgtca tagcggagtg cagcttgatg aacgttccgt caggcagtgc ccaaagtcgt
     5041 tcagtcgctt caggccgggc gaagatgacg tcgggaccaa gcggcgcgtt gcccatcttg
     5101 acgcctgctt tcggcatttc cttcgacagc cgatcgaaca tcgccttggc agcaggaaat
     5161 acgggaacga tcaccattgc ggccttgctg tgcagataag gccccagcat gccctgaaag
     5221 tctttcctgc tggtttcagg ttttagtttc agcaaaacca cggcactatc cctcaccgag
     5281 ttcggcgaag gcgtagcaac aatcaaatcc aacttctgca aggggccttg cacattaggt
     5341 tccgagagcc ggaaggcggc aatctctttt ccgtgcgtcc acgcgatcgt tcgcgggctt
     5401 gcggagtctc ccatgtcgaa tgaaagtacc ggggctttgg tcactgctcg cgattgcaag
     5461 cttgccgttt ggatgtactg cccattctcc gccgccggcg caacgccgta ctgctcgagt
     5521 tttcgagcga tgaattccgc ggcaagatac tcgtcgatgg tgccgctgcc gcggccatgc
     5581 agtttgggtc cggcgaggta ctcgagattc tcgcggacac aggcagcgca caaagcagac
     5641 gcgggacttg caaccttttc gggcggcgca gactggcccg gtaccgccga tgccgcgagc
     5701 tgaacacggg cgggcgcgag ctgggcggcg gcagaaatcg caaacgcaag aatgaccagg
     5761 gcaataaggg aagtcgagat aaaacgcgct cgatacgggg aggacttcaa gcgagggatt
     5821 ccttcgtgca aactaaattc caatccagcc agcgcggatc gccaacaggc tcaccacggc
     5881 aaccacgatc cacagcagca cgccttgcag cagcggacgc ggaccaacct tgcgcaaggc
     5941 ggccggcgat aggctggcgc cgatcaggaa gagcgtgatg atcagcatca gcttgccggc
     6001 gttgctcagg aatttgtacg cgatggcgcc ggggaacaaa taggtgttcg ccaccgccgc
     6061 caggcaaaaa aacagaatga accacggcca ttggatgcgc gtcttgctgc ccttgaccgc
     6121 tgcggtt
//
LOCUS       DASRKK010000652        13962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_63590, whole genome shotgun sequence.
ACCESSION   DASRKK010000652 DASRKK010000000
VERSION     DASRKK010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63590"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2232
                     /locus_tag="VFC15_15920"
     CDS             193..2232
                     /locus_tag="VFC15_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZM11695.1"
                     /translation="MNTSLLSRTVIKRPDRVRLQGRILFLTEDPELIKRQLAGEDLPW
                     NTQHPERNPKLRDDISTDEITPAHICFFFDETLGEFPYTGLKCGSETPIKRSDVKRGG
                     FVCCVSGKRRGKGSSREQSPYAEMAAGIKLVIGENIERIYKQNCQNLGLLAATDFSLI
                     DRIRDGQEIELREFTTGEDEITQQVIEYGGLFPFNVARLQGKVFLPPIATKPRPMTIA
                     EKIFARHMVHGACQVGVEAVKPGDTGFARTDLRFSHEYVTPMAAIFYDHYLGKEEPVN
                     DAGTILFFRDHLTFLDEVLSEEKRKMGLLDLATQLKLKQQDFAKSRGIKLHGELRDRK
                     GSEGICHSIVTESYALPGQLIVGSDSHTPHAGATGCVAFGIGTTDVFNSWFTKDVRVK
                     VPESVKVVIRGKRRDNATAKDFMLKLLSMEYIRSGKALAKVIEYSGEAIAALGVDERA
                     TMTNMAAEIGGFTGIVAPDPKVVEFLIERRGMGGNVAQTLIEGLQSDPDAHYAHVIEL
                     DANDIYPMVATPGDPGNGKFVRELNTPVPVEIAYGGTCTAGKNEDMDMYAEVLSAALK
                     QGRRVAESCQFYIQFGSQETREYCMRKGYLEIFENAGAQVIEPSCGACINAGPGVSTR
                     SDQVVISAQNRNFPGRSGPGLMYLGSPLTVAASAVAGYIVEYEPAGQREPVGVRE"
     gene            2379..2639
                     /locus_tag="VFC15_15925"
     CDS             2379..2639
                     /locus_tag="VFC15_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZM11696.1"
                     /translation="MAYVIAEPCINTKDTACVDACPVDCIHPKKDSEKFAGEEMLYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWKEYTERNAKYFGR"
     gene            complement(2696..3046)
                     /locus_tag="VFC15_15930"
     CDS             complement(2696..3046)
                     /locus_tag="VFC15_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM11697.1"
                     /translation="MPSKTVLCVDDEKIGLRVRKIMLESRGYAVLTATNGPEGLKVFD
                     ENHVDLVVLDYFMPDVNGGEVASEMRRRRPDVPIVFLSAYFSLPPEALELANAFITKG
                     DPPDVLIDRIQQLL"
     gene            3330..4478
                     /gene="gcvT"
                     /locus_tag="VFC15_15935"
     CDS             3330..4478
                     /gene="gcvT"
                     /locus_tag="VFC15_15935"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZM11698.1"
                     /translation="MPPETQSTPTVRKTALNAIHRQLGAKMVDFSGWDMPVEYPKYGG
                     LMKEHLAVRSGVGVFDVSHMGDIRVHGPQALAAVQHITMNDASKLTIGQCQYSAMLYP
                     QGTFVDDVIVHRLGESDYLLVINAGTREKDVNWVTENTKQFDCKVEHLSDDYTQIAIQ
                     GPKGAATLRKLTDADLSQVKFYWFTHGTVCGLPNTLIARTGYTAEDGFEIYIPSDEAT
                     SARVWNEVMQAGEEFGIVPCGLGARNTLRLEGALSLYGHEISEAINVFEARLDRFLKM
                     EKGDFVGREVLTRAKASGNKRTLVGLESVERGIPRDGYKVLNLDGKEIGCVTSGSYAP
                     FLKKNIALAYVPCELSAVDSEVAVEIRNQPVKCKVVPIPFYKRPKKQS"
     gene            4531..4920
                     /gene="gcvH"
                     /locus_tag="VFC15_15940"
     CDS             4531..4920
                     /gene="gcvH"
                     /locus_tag="VFC15_15940"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZM11699.1"
                     /translation="MAYPTEYKYTKEHEWLKVDGTTGTIGITDHAQNSLGDIVFVELP
                     KVGAEITAGQTFGSVESVKAVSDLYAPVSGTVTEINEALNTSPEEVNNDAHAAWMIKV
                     NLKNPAEAGSLLSAADYEKFAADEAGH"
     gene            5019..6344
                     /gene="gcvPA"
                     /locus_tag="VFC15_15945"
     CDS             5019..6344
                     /gene="gcvPA"
                     /locus_tag="VFC15_15945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZM11700.1"
                     /translation="MRYLPKSQLDREEMLKDIGVSTIDDLFAAIPAEYRFTDDLKIPR
                     SYAESEIVEFFRERAAENGQGYASFLGAGAYSHYRPVVIDALVSRGEFFTAYTPYQPE
                     IAQGTLQSIFEFQSMICELTGMDVANASMYDGSTGAAEGLMMAVRVTGRHKSVIATSV
                     HPEYREVITTYARHQGMPLATVAYGKNGRVDLDALDKAVDNETAGVLIQSPNFFGVIE
                     DVAAIADIVHKKGALLIVSIAEAVSLGIVKPPAEADIVSLEAQSFGVPLGFGGPYCGV
                     MATRDKFVRQMPGRLVGETTDRNGKRGFVLTLSTREQHIRREKATSNICTNQALVALM
                     CNIFMTIYGREGMKELAQHNLAKAHYAAEQFKKSGAKLVFGDAPFFNEFVIETSGDPR
                     AVNEKLLEQKIIGGFPLKKFYPELSKASLWCCTETMTRASIDAAAKEVA"
     gene            6344..7864
                     /gene="gcvPB"
                     /locus_tag="VFC15_15950"
     CDS             6344..7864
                     /gene="gcvPB"
                     /locus_tag="VFC15_15950"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZM11701.1"
                     /translation="MGINDKITKATTHISQNEGLIFEKSSPGKKAYKLPPLDVPAVDA
                     EKLLGDLTRDDLGQMPEVSEIEIIRHFTRISTWNYAVDHGMYPLGSCTMKYNARVNET
                     VARLTGLAEAHPYQPEKISQGALRIIKMLSDQLIEITGMDAITLQPAAGAHGELTGIL
                     LVRAYLESQGNPRKKILVPDSAHGTNPATAAMCGYAVENLKSNAAGMVDIASLEAQVN
                     EDVAALMLTNPNTLGVFEQEIHKIADLLHSKGALMYMDGANMNALVGKVRPGDFGADV
                     MHLNLHKTFSTPHGGGGPGSGPVACKKILEPFLPTPVLITKPDGTLGFDYNRPQSIGR
                     VRMFYGNFGMFVRALAYIMANGPEGLRQTTEDAVLNANYIRKNLEGVYDLPYQTPTMH
                     EVVFSDRIQARNGLKTGDIAKRLIDYGFHPYTVSFPLVVPGALMIEPTESESKEELDQ
                     FIDAMKAVAEEAENSPEIVASAPHNTRVSRMDETAAARKPVLRWKPQAKEAELAIK"
     gene            complement(7943..8440)
                     /gene="rpsT"
                     /locus_tag="VFC15_15955"
     CDS             complement(7943..8440)
                     /gene="rpsT"
                     /locus_tag="VFC15_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZM11702.1"
                     /translation="MPFASKTVSFMMLPKRPWRTPAASLIGWPDWEKEAGPSGACPEN
                     LPARFAHFAHRPVRSGIRHTSRFLLKEYNTVMADHYSALKRARQTEKKTTQNRANKSR
                     LRSSLRSLREAIAKGDKAEAQKAYNATTSVVDKGVQKGVLHKNTASRYKARLNKRVKA
                     VGAAK"
     gene            8635..9741
                     /locus_tag="VFC15_15960"
     CDS             8635..9741
                     /locus_tag="VFC15_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZM11703.1"
                     /translation="MKTNIEISPNIETLFGTRDENLRLLEDGLNVVIGMRTDSIEIEG
                     APNDVARAGQVFSDYQHLLRNGFTFVNGDLGAMLKVLTSDGTATLRGLAEAGKQRSFG
                     KRTVQPKSINQRRYLEAIEKNDMVFGVGPAGTGKTYLAVAMAISALMSKRVNRIILAR
                     PAVEAGERLGFLPGTLQEKVDPYLRPLYDALYDMLDPDKVDRYLEKNVIEIAPIAFMR
                     GRTLNDAFVILDEAQNTTSEQMKMFVTRLGFNSKAVITGDVTQIDLPNPSRSGLVEAG
                     EILKNVEGIRFCYFDEGDVVRHHLVQRIVRAYEDHKGRNEQLSLSLDVRGSNGFAAPP
                     AGNGSSYTPSVDREIVLAEVPPVAGPETGNRISE"
     gene            9801..10397
                     /gene="ybeY"
                     /locus_tag="VFC15_15965"
     CDS             9801..10397
                     /gene="ybeY"
                     /locus_tag="VFC15_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZM11704.1"
                     /translation="MSYLGRKSKQTSARALSLFATTAQHALGLDGEVNIYVTSSREMQ
                     DLNRRYRRKNKPTDVLSFPSRLPGVAGDIAISLEIAAVNAAEIGHSLATEVKVLILHG
                     LLHLAGYDHEADNGEMLARETAMRQELKLPVGLIERTHAATAKAAETARRPKATARKA
                     AAARSSSARRQAVTSQRTPASAKRTASGKTGKREGRRA"
     gene            10394..11668
                     /locus_tag="VFC15_15970"
     CDS             10394..11668
                     /locus_tag="VFC15_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZM11705.1"
                     /translation="MNLPLFILLLFLLAVLALVSYVNRLHHEMGKFLSREFQENIDAY
                     EQSVEPRLGFTRERASLSMAVLTQICMAAIGVLIGYLLFKDNGWVGLDLVQGAVSLIF
                     IIVIFNRLLPHIFFVRTKGEWLAKFVPVLRILIYLAVPVTMIVGFALSVATLSKQHGE
                     SEPEHPSEAVDALIEAGQEEGILEESDRDLIQSVVEFGDKTVREVMTPRPEIAAVPIT
                     MTIAEFTEMLNKSPYSRMPVYEGDIDHIKGIMHTKDVLQVADTEAGKKTVADLMKTDV
                     YFVPETKLGSELLREMQQENIRMAIVADEYGGVAGLVTIEDLVEEIVGEIRDEHEKSD
                     IVKESDSSYIFNGNTDLDLLEKLLGGARPDDKEATTIGGLVSELAGRIPAAGEVFEDN
                     GLRFEVLDSTERRVERVRVSLQNVSAPKQIRA"
     gene            11670..12632
                     /gene="era"
                     /locus_tag="VFC15_15975"
     CDS             11670..12632
                     /gene="era"
                     /locus_tag="VFC15_15975"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZM11706.1"
                     /translation="MAFRSGFISIIGRPNAGKSTLLNALVGEKLAIVTRKPQTTRNRI
                     QGIVNVEKKGKRPAGQLVLIDTPGVHKPINSLNRRMMKEVYDALEGCDLLLVIVDATG
                     KFDEGDQFVLNLAKKTNVPIVLLLNKIDLIPKERLLPLIAQYKELHDFVDVIPISALK
                     EKGLDTLLDVIIKALPEGPRYFPKDQITDQPERFLVSELIREQVLQYTSQEVPHATTV
                     MIDQYEEGSKLTRIAATIYCERDGQKAILIGKQGANLKKIGTNARLQIERLLATKVFL
                     ELFVKVSAGWRDSRAFVEETDWRRQLENIAGGMLTDQPDPKPED"
     gene            complement(12640..>13962)
                     /locus_tag="VFC15_15980"
     CDS             complement(12640..>13962)
                     /locus_tag="VFC15_15980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM11707.1"
                     /translation="SEIRSSLQGVLEDEGYKTVLAESAEECLATLARRAVDVVLLDIW
                     LPGIDGLEALERIVEMDDPPEVIMISGHGNIETAVRATKLGAFDFLEKPLSLVRTLIV
                     LKNALDSKHLRSENLEYKKQFQARSVIVGDSIPMKALRQQTALMAPTNGRVLIYGESG
                     TGKELVARAIHAQSLRKNEMFVEVNCAAIPEDLIESELFGHRVGSFPGATSDKEGKFQ
                     KADGGTLFLDEVGDMSLKTQSKVLRTLDEGRFVPVGEEEPITADARVIAATNKDLEDE
                     ISKGNFREDLFYRLNVIPFYVPPLRERKEDIAQLARHFLKEFSHTYNRRPREIADDAL
                     ETLTRYTWPGNVRELRNLIERIVIMNPAVARLERKHLPTLVYREGGRRSLGEFSSLHE
                     ARASYERDYILKKLDENLGNVSRTAEVLGLERSHMYRKMKALGIAVKE"
BASE COUNT         3095 a         3906 c         4221 g         2740 t
ORIGIN      
        1 tcaataacgt tctggccagt gcaccctatt cgacgcaact cgcgatgaac gaaattgacg
       61 cggtgtcgca ggtgatggac cgggtggcga atgcggtgtt tggggaggtc aagaagggcc
      121 ccgcgccttt gccagagtag cccgaaatcc ggttctagcc ttggcgggct caatctgcga
      181 taaaatcttg cggtgaatac gtcgttgttg agccgcaccg tgattaagcg tcctgaccgg
      241 gttcgtctgc aggggcggat acttttcctc accgaagatc ccgaactgat caagcgtcag
      301 ctggcgggag aagacctgcc gtggaacacg caacatcccg agcgcaatcc caagctgcgc
      361 gacgatattt cgaccgacga aatcaccccg gcgcacatct gcttcttctt cgacgagacg
      421 ctgggagagt ttccctacac cggcttgaag tgcggtagcg agacgccgat caagcgcagc
      481 gacgtgaagc gcggcggttt tgtgtgctgc gtcagcggca agcgccgcgg caaaggatcg
      541 agccgcgaac aatcgcctta cgccgagatg gcagccggaa tcaagctggt gatcggagaa
      601 aatattgagc gcatctacaa gcagaactgt cagaacctcg ggttgctggc ggcgaccgat
      661 ttcagcctga tcgacagaat ccgcgacggc caggaaatcg agttgcgcga gttcaccacg
      721 ggcgaagatg aaattaccca gcaggtgatc gaatacggcg gactgtttcc cttcaacgta
      781 gcccgcctgc agggcaaggt atttcttccg ccgatcgcca ccaagccgcg tccgatgacg
      841 atcgcggaga agatattcgc gcgccacatg gtgcatggcg cctgtcaggt gggcgttgaa
      901 gcagtgaagc ctggggacac cggcttcgcg cggaccgatc tgcgtttcag ccacgaatac
      961 gtcaccccga tggcggcgat tttctacgat cactatctgg ggaaagagga gccagtcaat
     1021 gatgccggca ccatcctgtt cttccgcgac cacctcacgt ttctcgacga agtgctgtcg
     1081 gaagaaaagc gcaagatggg tctgctcgac ctggctacgc agttgaagct gaagcagcaa
     1141 gacttcgcga aatcgcgcgg catcaagttg cacggcgagt tgcgcgaccg caaaggctca
     1201 gaagggatct gtcactcgat tgtcaccgag agctatgcgc taccgggcca gctcattgtt
     1261 ggttccgact cgcacacacc gcacgccggc gcgacgggat gcgtggcgtt tggcatcggg
     1321 acgaccgacg ttttcaactc ctggttcacc aaagacgttc gcgtgaaggt acccgagtcg
     1381 gtgaaggtgg tgattcgcgg caagcggcgc gacaacgcga ccgccaagga ttttatgctg
     1441 aagctgctgt cgatggagta catccgcagt ggcaaggcgc tggcgaaagt cattgagtat
     1501 tccggggagg cgattgcggc cctcggcgtg gacgaacgcg ccacgatgac caacatggcg
     1561 gcagagattg gcggcttcac cggcatcgtt gcgcccgatc cgaaggtggt tgaatttctg
     1621 atcgaacgtc ggggaatggg cggcaacgtg gctcagacac tgatcgaggg cctgcagagc
     1681 gatcccgatg cgcactatgc gcacgtgatt gaattggacg caaacgacat ttatccgatg
     1741 gtcgcgacgc cgggtgatcc gggcaacgga aagtttgtgc gcgaactcaa cacgccggtt
     1801 ccggtggaga tcgcatacgg cggaacctgc accgccggca agaatgaaga tatggacatg
     1861 tacgcagagg tgttgagtgc ggccctgaag cagggaagac gggtggcgga gagctgccag
     1921 ttctatattc agttcgggtc gcaggagacc cgcgaatact gcatgcgtaa aggttatctt
     1981 gagatcttcg agaacgcggg agcgcaggtg atcgaaccca gttgtggggc ctgcatcaac
     2041 gccggtccgg gcgtttcgac ccgttctgac caggtggtga tcagcgcgca aaaccgcaac
     2101 ttcccgggtc gcagcggtcc gggcttgatg tacctgggga gcccgctcac ggtagccgcc
     2161 agcgcagtcg cgggctacat cgtggagtat gaaccggcgg gccagcgcga acccgtggga
     2221 gtccgggaat aggttagtct ctgcggttga tcgacaccgc gcagcaggag acgcggacaa
     2281 cgacgcggcg tcacgctaga gcgcaatttt tgataagatc gctattccac tctgattcag
     2341 aatagccggc ttgggaaaag ctcagggaga acagttttat ggcctatgtg atcgcggaac
     2401 catgtattaa cacgaaggac accgcttgcg tcgatgcctg tccggtggat tgcattcacc
     2461 ccaagaaaga cagcgagaaa ttcgcaggcg aggaaatgct gtacatcgat ccggtcgaat
     2521 gcatcgattg tggagcgtgc gtacccgtgt gcccggtatc cgcgatcttc gcgctcgatg
     2581 acctgcccga gaagtggaaa gagtacaccg agcgtaacgc caagtatttc ggccgctagg
     2641 atttttggtg gtatgcgaaa gggcgcactg cgagtgcgcc cttcttgcgt gtggattaga
     2701 gcagttgctg gattcgatcg atcagcacat cgggagggtc gcccttggtg atgaaagcgt
     2761 tggctaactc cagtgcctca ggaggaagag aaaagtacgc ggagagaaat acgatcggaa
     2821 catcgggtct gcggcggcgc atttccgaag cgacttcccc gccattcacg tccggcatga
     2881 agtaatccag caccacgaga tcgacgtggt tttcgtcgaa aaccttgagt ccttccgggc
     2941 cgttcgtggc agtcagcact gcgtaaccgc ggctctccag catgatcttg cggacacgca
     3001 gaccgatctt ctcgtcgtcg acgcacaaca cagtcttcga aggcactgac tttatccttg
     3061 taactggctc gcgttcagcc tctgacttca agaatcccga agtagacatc gcttatatat
     3121 ggccgcttcg cactcctcca gaaaggcaga ggaagacggg tatgagaagt gactgcctga
     3181 tttgtactgc aatcggccaa ccttgaccat agtcaaaatg tcgtaggaaa gtgctatgcc
     3241 ggtcgttgac cgcgcgaaaa agcgcgtgtt accgtacttg gttacaccat cctgtgttgg
     3301 gcgttggtaa tgcgccgaag ggaagttgtt tgcctcccga aacccagtcc actccaaccg
     3361 tccgcaagac cgccttaaat gccattcacc gccagttggg ggcgaagatg gtcgatttca
     3421 gtggctggga tatgcctgtc gagtacccga agtatggtgg cttgatgaag gagcacctcg
     3481 cagtccgcag cggcgtaggt gtctttgatg tcagtcacat gggagacatt cgggtgcatg
     3541 ggccgcaggc actggcagcg gtccagcaca tcaccatgaa cgacgcttct aaactgacta
     3601 ttggtcagtg tcaatattcg gcgatgctgt atccccaggg cacattcgtg gacgacgtga
     3661 tcgtccatcg cttgggagag agtgattacc tattagtaat taatgccggg acgcgggaaa
     3721 aagatgtcaa ctgggtgacc gagaacacca aacagttcga ctgtaaggtc gaacatctca
     3781 gtgacgatta cacgcaaatc gccatccagg ggcccaaggg cgcggcaacc ctgcggaaat
     3841 tgacggacgc cgatctgtcg caagtgaagt tctactggtt cacgcacgga acggtgtgcg
     3901 ggctacccaa cacgctgatc gcgcgaactg ggtacaccgc cgaggatggg ttcgaaattt
     3961 atatcccgtc ggacgaggcg accagcgcgc gggtctggaa cgaagtgatg caagccggcg
     4021 aagaatttgg catcgtcccc tgcggactgg gtgcgcgtaa cacgttgcgt ttggaaggcg
     4081 cgctgtcgtt gtatgggcat gagatctccg aggccattaa tgtttttgag gcgaggcttg
     4141 accgtttttt gaagatggag aaaggcgact tcgttggccg cgaagtctta acgcgggcga
     4201 aagcatctgg taacaagcgg acgctggtgg gattggagtc ggtggagcgt ggtattccgc
     4261 gcgatggcta caaggtgctc aacctcgacg gaaaagagat tggctgcgtc accagcggat
     4321 cgtatgcgcc gttcctgaaa aagaacatcg cattggccta cgtaccgtgc gagctgtcgg
     4381 cagtcgatag cgaagttgca gttgagatca ggaatcagcc ggtgaagtgc aaggtagttc
     4441 cgataccgtt ttacaagcgc cctaaaaagc aatcataagt ttgcctttga cgaacatccc
     4501 ccggtgtccg gtgtggagaa gggaatcaaa atggcctatc ctacggaata caagtacacc
     4561 aaagagcatg agtggctgaa ggtggacggc acgaccggca ccatcggcat cactgaccac
     4621 gcgcagaatt cgctaggcga cattgtgttt gtcgagttgc cgaaggtcgg tgccgaaatt
     4681 acggcgggac aaacattcgg ctcggtggag tcagtgaagg ccgtgtctga tctgtacgca
     4741 ccggtgtcag gaaccgtcac cgaaatcaac gaagcgctca acacctcgcc ggaagaggtg
     4801 aacaatgacg ctcacgcagc atggatgatc aaggtcaatc tgaagaatcc ggcggaggcg
     4861 ggcagtcttt tgtcggcagc cgattacgaa aagttcgcgg cagacgaagc cggccactga
     4921 gctactcccc cgcagtgcgc tctaaccgac gtgttcgaag tgtagccgat gcagaatcca
     4981 tacgcggctg cacatttatc tgaaagttat aggtacccat gcgctatctt cctaaatcac
     5041 aattagaccg cgaagaaatg ctgaaggaca tcggcgtcag cacgatcgat gacctgttcg
     5101 ccgcgattcc agccgagtat cggttcactg acgacctcaa gattccgcgc tcgtacgcgg
     5161 agagcgagat tgttgagttc ttccgcgaac gcgctgccga gaatggacag ggctacgcgt
     5221 cgttcctcgg agcgggggcg tactcgcact accgcccggt ggtaattgac gcgctggtct
     5281 cacgcggcga gtttttcacg gcatacactc cgtatcagcc ggaaatcgca caaggcacgc
     5341 tgcaatccat cttcgagttt cagtcgatga tctgcgagct gaccggcatg gatgtggcga
     5401 acgcttctat gtacgacggc tcgaccggcg cggcggaagg cctgatgatg gccgtccgcg
     5461 tgaccggccg ccacaagtca gtgatcgcca ccagcgtgca tccggagtat cgcgaggtca
     5521 tcaccaccta tgcccgtcac cagggaatgc cgctggcaac ggtggcctac ggcaagaacg
     5581 gtcgcgtcga tctcgatgca ctcgacaagg ccgtggacaa cgaaaccgcg ggcgtgctga
     5641 ttcagtcgcc gaacttcttt ggagtgatcg aagacgtagc cgcgattgcg gacatcgtcc
     5701 acaagaaggg tgcactgctg attgtctcga tcgccgaggc ggtttcactg gggatcgtga
     5761 agcccccggc tgaggccgat atcgtgagcc tggaagcgca gtcgttcggc gtgccattgg
     5821 gcttcggcgg accgtactgc ggagtgatgg ctacacgaga caagttcgtg cgccaaatgc
     5881 cggggcgtct ggtaggagag accacggacc gcaacggcaa acgcggattc gtgttgacgc
     5941 tctcgactcg cgaacagcac atccggcgcg agaaggcgac ctcaaacatc tgcaccaacc
     6001 aggcgctggt cgcactgatg tgcaacatct ttatgaccat ctacggccgc gaaggcatga
     6061 aggaactggc gcagcacaac ctggcgaagg cgcactacgc cgcggaacag ttcaaaaaga
     6121 gcggcgccaa gttggtgttt ggcgacgcgc ccttcttcaa cgagttcgtg atcgagacca
     6181 gcggcgaccc gcgggccgtc aacgagaaac tactggagca gaagatcatc ggcgggttcc
     6241 cgctgaagaa gttttatccc gagttgagca aagcttcgct gtggtgctgc accgagacca
     6301 tgaccagggc ttcgatcgac gcggctgcga aggaggtggc gtaatgggta tcaatgacaa
     6361 gatcacgaaa gccaccacgc acatcagcca gaacgaagga ctgatctttg aaaaatcatc
     6421 gccgggcaag aaggcctaca agctgccccc gctcgatgtg ccagcggtgg atgccgagaa
     6481 gttgctcggc gacctgacgc gcgacgacct cggccagatg cccgaagtga gcgagatcga
     6541 gatcatccgc catttcacgc gcatctccac gtggaactat gcagtagatc acggcatgta
     6601 tccgctgggc tcctgcacca tgaagtacaa cgcgcgcgtg aacgagaccg tcgctcgtct
     6661 gacgggattg gctgaggcgc atccctacca accggagaag atttcgcagg gcgcgctgcg
     6721 catcatcaag atgctcagtg atcagctcat cgaaattacc ggcatggacg ccatcacgct
     6781 gcagccggcc gctggagcgc acggcgagct gacgggaatc ctgctggtac gcgcctatct
     6841 agagtcgcag ggcaacccgc gcaagaaaat cctggtgcca gattcggcgc acggaaccaa
     6901 tcccgcgacc gcggcgatgt gcggctacgc ggtcgagaac ctcaaatcga acgcagccgg
     6961 catggtggac atcgcgtcgc tggaagcgca ggtgaacgaa gacgtcgccg cgctgatgct
     7021 caccaatccc aatacgctgg gcgtgttcga gcaggagatc cacaagattg ccgatctgct
     7081 ccactccaaa ggcgcgctga tgtacatgga cggcgcgaac atgaacgcgc tggtgggcaa
     7141 ggtgcgtccg ggagacttcg gcgccgacgt catgcacctc aacctccaca agacgttttc
     7201 gacaccgcac ggtggaggcg gtccgggctc cggccccgtg gcttgcaaga aaattctgga
     7261 gccgttcctg ccgacacctg tgctcatcac caaaccagac ggcacgctgg gcttcgacta
     7321 caaccgtccg caatccatcg gacgggtgcg catgttctac ggcaattttg gaatgtttgt
     7381 ccgcgcgttg gcctacatca tggccaacgg gcccgaggga ttgcggcaaa ccacggaaga
     7441 cgccgtgctg aatgcgaact acatccgcaa gaatctggaa ggcgtgtacg acctgccgta
     7501 ccagacgccg accatgcacg aggtggtgtt cagtgaccgc atacaggcac gcaacggact
     7561 gaagaccggc gacatcgcca agcgcctgat cgattacggc ttccatccct acacggtgag
     7621 tttcccgctt gtcgtgccag gcgcgctgat gatcgagccg acggagagcg agtcgaaaga
     7681 agagcttgat cagttcatcg acgccatgaa agccgttgct gaagaggctg agaactcgcc
     7741 cgagatcgtg gcctccgcgc ctcacaatac gcgggtctca cgcatggacg aaaccgccgc
     7801 agcgcgcaag ccggtgctgc gctggaagcc gcaggcgaaa gaggcggaat tggcgatcaa
     7861 gtagtctcgg cgcagtaacc agcgggaaat gaaaaggcgg accgattggg gtccgccttt
     7921 aaaccttggt ggttggagcg aactacttgg ctgcgccgac ggcctttacc cgcttgttga
     7981 ggcgggcttt atagcggctg gcggtgttct tgtgcagaac acccttctgg acgcccttgt
     8041 ccaccacaga cgtggtggcg ttgtacgcct tctgcgcctc ggccttgtcg cctttagcaa
     8101 tggcctcgcg caaagagcgc aaagagctgc gcaggcggga cttattggcg cggttctgcg
     8161 tcgtcttctt ttctgtctga cgggcgcgct tcagcgccga gtagtggtct gccataactg
     8221 tgttgtattc ctttagtaga aatctcgacg tatgccgaat acctgatctt acaggacgat
     8281 gggcgaaatg ggcaaaccgt gcgggtaaat tctcgggaca tgcgcccgac gggcctgctt
     8341 ctttttccca gtccggccat ccaatcaaac tggcagcggg cgtgcgccag ggtcgcttgg
     8401 gaagcatcat gaacgacacg gtttttgagg cgaacggcat tgacgccgtt tcggttcgag
     8461 gttaggctaa cgggcaatgt cagttttcgc ttcggcgaac aattcggatg tacttcctgt
     8521 tttcgactcc aagaaattcc tgcgcgacca aatttgtcag accggaaact ccggtttggc
     8581 gaggcatcca cgcttgaacc caccgggcct caggaggcga gcgaccgacc ctaaatgaag
     8641 accaacatcg aaatcagccc aaacatcgaa actctctttg gcacacgcga cgaaaatttg
     8701 cgtctgctcg aagatggcct gaacgttgtc atcggaatgc gaaccgactc catcgaaatc
     8761 gagggagcgc cgaacgatgt cgctcgcgcc ggacaggtat tttccgatta ccagcacctg
     8821 ctacgcaatg gctttacatt cgtgaacggc gatcttggcg ccatgctgaa ggtgctgact
     8881 tccgacggca cagccacttt gcgcggcctg gcggaagccg gcaagcagcg ctcgtttggc
     8941 aagcgcacgg tgcaacccaa gagcattaac cagcggcggt atctcgaggc catcgagaag
     9001 aacgacatgg tcttcggcgt cggccctgcg ggaaccggca agacgtatct cgcggttgcc
     9061 atggcgatct cggcgctgat gtcgaagcgc gtgaaccgca tcatcctggc gcgtccggcg
     9121 gtggaggccg gcgagcgact gggattcctg ccaggtacgc tgcaggagaa ggtcgatccc
     9181 tacctgcgcc cgttgtatga cgcgctgtac gacatgctcg atccagacaa ggttgatcgc
     9241 tacctggaaa agaacgtcat cgagattgcg cccattgcgt tcatgcgcgg ccgcactctc
     9301 aacgacgcgt tcgtcattct cgatgaggcg cagaacacca cgtcggagca gatgaagatg
     9361 ttcgtcaccc gactgggctt caactccaag gcagtcatca ccggggacgt cacgcagatc
     9421 gatttgccca accccagccg cagcggactg gtcgaagccg gagagatcct caagaacgtc
     9481 gagggaattc gcttctgcta ctttgacgaa ggcgacgtgg tgcgccatca cctggtgcag
     9541 cgcattgtcc gtgcctacga agatcacaag gggcggaacg agcagctttc cttgtcactc
     9601 gacgtgaggg gcagcaatgg attcgcggcg ccaccagccg ggaacggaag cagttacacg
     9661 cccagcgttg accgtgagat tgtgctggcg gaggtcccgc cggtggccgg tccagagacg
     9721 ggaaatcgca tttccgagta aaaccttgct accatgttgt gggagggctc aatcgctctc
     9781 ccatttcttc ttgaacggaa gtgagctacc tggggcgaaa atcgaaacaa accagcgcgc
     9841 gggccctcag cctgtttgcg accacggcgc agcatgcgct tggactggac ggcgaagtga
     9901 acatctacgt cacctcgagc cgtgagatgc aggacctgaa tcgccgttat cgacgtaaga
     9961 ataagcctac ggatgtgctg tcgtttccat cgaggttgcc gggagtcgcc ggcgacattg
    10021 cgatttcgct ggagatcgcc gcagtcaatg cggctgagat tgggcattct ttggccaccg
    10081 aagtgaaagt cctcatcctg catggactgc tgcacttggc gggctacgat cacgaagctg
    10141 acaacggcga aatgctggct cgtgaaacgg ccatgcggca agaactcaag cttcctgtcg
    10201 gtttgattga gcgcactcac gccgcaaccg ccaaagcagc ggaaacggcg cgcaggccca
    10261 aagccacagc gaggaaagcc gcggcagctc ggtcttcgtc ggcgcgcaga caagctgtca
    10321 ccagccagcg cacacccgct tcggcaaagc gcactgcgtc gggcaagact ggcaagcgcg
    10381 aaggcaggcg cgcatgaacc ttccgttatt catcctgctg ctctttctgc tggcggtgct
    10441 cgcgttggtg tcctacgtca atcggctcca ccacgagatg ggcaagtttc tgtcgcggga
    10501 attccaggaa aacatcgacg cctatgaaca gtcggtggaa ccgcgcctgg gtttcacccg
    10561 cgagcgcgcg tcgctctcga tggctgtgct gacccagatt tgcatggctg ctatcggagt
    10621 tctcatcggc tacctgctgt tcaaggacaa cggttgggta ggcctcgatt tggtgcaggg
    10681 tgccgtcagt ctcatcttca tcatcgtgat cttcaatcgc ttgctgccac acatattctt
    10741 cgtgcgcacc aagggcgagt ggctggcaaa gttcgttccg gtgttgcgga tactcatcta
    10801 ccttgcggtg ccggtcacga tgatcgttgg ttttgcactt tcggtagcaa ccctctcgaa
    10861 acagcatggc gaaagcgagc cggaacatcc ctccgaggcc gtggacgcgc tgattgaagc
    10921 tggacaggaa gagggcatac tcgaggagag tgaccgcgac ctcatccagt cggtggtcga
    10981 gttcggcgac aagacggtgc gtgaagtcat gacgccacga ccggaaatcg cggcggtgcc
    11041 tatcacgatg accatcgcag agtttactga gatgctgaac aagagtccct actcgcgcat
    11101 gcccgtgtat gaaggcgaca tcgatcacat caaggggatc atgcacacca aggacgtgct
    11161 gcaagtcgcc gacaccgaag ccggaaagaa gacggttgcc gacctgatga agacggatgt
    11221 ttacttcgtt cccgaaacca agttgggcag cgagttgctc cgtgagatgc aacaggaaaa
    11281 catccggatg gcaatcgtgg ccgacgaata cggtggcgtc gccggactgg tcacgatcga
    11341 ggacctggtc gaggagattg tgggcgagat ccgcgatgag cacgagaagt cggacatcgt
    11401 gaaggagagc gacagctcgt acatcttcaa cggcaatacc gatctcgacc tgctggaaaa
    11461 actgctgggc ggcgcgcggc cggacgacaa agaggcgacg acaattggcg ggctggtgag
    11521 cgagttggcg ggtcgcatcc ccgcggcggg cgaagtcttc gaagataacg gtttgcgttt
    11581 tgaggtgctg gactcaaccg agcgtcgcgt cgagcgcgtg cgtgttagtc ttcaaaacgt
    11641 gtctgctccc aagcagattc gagcctgaca tggcattccg cagcggattc atttcgatca
    11701 tcgggcgtcc caacgcaggg aaatcaacgc tgctcaatgc gctcgtaggc gagaagctgg
    11761 cgatcgtcac gcgcaagcca cagaccacgc gcaaccgcat ccagggcatc gtcaacgtcg
    11821 agaagaaagg gaagcggcct gccggacaac tggtcctgat cgacactccg ggcgtgcaca
    11881 agcccatcaa ctccctcaac cgtcgcatga tgaaagaggt atacgatgcc ctggagggct
    11941 gcgacctgtt gctggtgatt gtcgatgcca cgggaaagtt cgacgagggc gaccagtttg
    12001 tcctgaatct cgcaaagaag accaacgttc ccatcgtcct gctgctcaac aaaatcgatc
    12061 taatccccaa agaaaggctt ctgccgctga ttgcgcagta caaagaactg catgatttcg
    12121 ttgacgtcat tcccatctcg gcgctcaaag aaaagggact tgacacgctg ctcgacgtca
    12181 ttatcaaagc gctgccggaa ggcccgcgct atttcccgaa agatcagatc accgatcagc
    12241 cggaacgatt cctggtgtcg gaattgatac gcgagcaggt gctgcaatat acctcgcagg
    12301 aagtaccgca cgccaccacg gtgatgatcg atcagtacga agagggctca aagctgacgc
    12361 gcatcgcggc gaccatctac tgcgagcgcg acgggcagaa ggcgatcttg atcggcaagc
    12421 agggcgcgaa cctgaagaag atcggaacca acgcgcggct gcagattgaa cgtctgctgg
    12481 ccacgaaggt ttttttggag ttgttcgtga aggtaagcgc gggctggcgc gattcgcgcg
    12541 ccttcgtcga agagaccgac tggcggcggc agttggagaa catcgccgga ggcatgttga
    12601 ccgatcagcc cgaccccaag ccggaagatt gatctcaggc tattctttta ccgcaattcc
    12661 cagcgccttc atcttgcgat acatgtggct gcgctcgaga ccgaggactt ctgcggtgcg
    12721 gctcacgttg cccagattct catccagctt cttgaggata tagtcgcgct cgtacgaggc
    12781 acgtgcctcg tgcaggctgg aaaactcgcc tagactgcgg cgtccgccct cgcgataaac
    12841 cagggtcggc agatgtttgc gttccagacg cgctaccgcg ggattcatga tcacaatgcg
    12901 ctcaatcaag ttgcgcagct cacgaacgtt tcctggccaa gtgtagcggg tgagcgtttc
    12961 gagagcatcg tcggcgattt cacgtggacg ccggttgtag gtgtgcgaaa actctttcag
    13021 aaaatgcctg gccaattgag cgatatcctc tttgcgctca cgcagcggtg gcacataaaa
    13081 cgggatgaca ttcaggcgat agaagaggtc ttcgcggaag ttccccttgg aaatttcgtc
    13141 ttccaaatct ttgttggtgg cggcaatcac gcgagcgtca gcagtaatgg gctcttcctc
    13201 gccaaccggc acaaagcggc cctcatcaag cgtgcgcagc accttggact gcgtcttgag
    13261 actcatgtcg ccgacttcat cgaggaagag tgttccgccg tcggccttct ggaatttacc
    13321 ttccttgtcc gacgtcgcac ccgggaagga gccaacgcga tgtccgaaca actcgctctc
    13381 gatcaagtcc tccgggatgg ccgcacagtt gacctcgacg aacatttcat tcttgcgcaa
    13441 gctctgagcg tggatggcgc gcgccaccaa ttcttttccc gtgcccgact cgccataaat
    13501 cagcacccgc ccattggtcg gcgccatcag cgccgtctgc tggcgcaggg ccttcatggg
    13561 aatgctgtcg cccacaatta cgctgcgcgc ctgaaactgt tttttgtatt cgaggttttc
    13621 gctgcgcaga tgcttggagt caagggcgtt tttcaaaacg atgagcgttc gcaccagcga
    13681 gagcggcttt tcgaggaagt cgaatgcccc cagcttggtg gcgcgcacgg cggtttcgat
    13741 gttgccgtgg cccgagatca tgatgacttc gggtggatcg tccatttcga cgatgcgctc
    13801 cagcgcttcc agaccatcga tgccgggtag ccagatgtcg agcaggacca catcgaccgc
    13861 cctgcgcgcg agcgtggcaa gacattcctc ggcgctctcc gccagcacgg tcttgtagcc
    13921 ctcatcctcc agcacgcctt gcagcgaact gcggatttcc ga
//
LOCUS       DASRKK010000653         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1432610, whole genome shotgun sequence.
ACCESSION   DASRKK010000653 DASRKK010000000
VERSION     DASRKK010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432610"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1727)
                     /locus_tag="VFC15_15985"
     CDS             complement(123..1727)
                     /locus_tag="VFC15_15985"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11708.1"
                     /translation="MKHDLQEERIAELEEQLGVLQKQFAVLQKQTATLLEQNHRLSEE
                     NQALRDELAVLKHQKPKPSIRPSRLHEGEGKERKNKGKRKPGKERKVDHTVVVKPEKV
                     PQGSRFKGYSDYVVQELVVKVEATLYRVEKWLTPEGKLVTGELPVALPVEGPGDHFGP
                     TARCFVLYQYYHAQVTEPLILEQLQEWGMQMSSGQLHRLITEGKEQFHQEKDAILRVG
                     LRVSRHIHVDDTGARHQGKNGYATHIGNELFAWFQTTESKSRINFFELLRAEQTDYVL
                     NDEALEYMAAQKLPKEPLAKLQPAVGQVFANKDQWQSALKGRGIAQERNVRIATEGAL
                     LGSVLEHGFNRDLAIVSDDAGQFNVFLHGLCWIHAERVFAKLVGFNDSQRQDLGQIRT
                     EIWQLYRDLKAYQLEPTPAAKLAIEDRFDALCATETCFTSLNLALKRMQRNKRELLLV
                     LDRPDLPLHNNLSEGDIREYVKRRKISGGTRSDDGRRCRDTFASLKKTCRKLGVSFWS
                     YLSDRLGGKKLIPALPQLIEARAQAP"
     gene            2006..>3163
                     /locus_tag="VFC15_15990"
     CDS             2006..>3163
                     /locus_tag="VFC15_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11709.1"
                     /translation="MTIAHWTPRTTISRQEQFLLKRLQRTRKLFAFLRLYRHQLFDES
                     FQDELAGMYRDTGAGKDAKPPALLAMALLLQGYLGTSDAEVVEMTVVDLRWQMVLDRL
                     GADEPAFSQGALQNFRERLIAADLDRRLLERTVELAKQTRAFDAKKLPKSLRIAIDSS
                     PLEGAGRVEDTFNLLGHAARKVVECAAAALQLSVEQVCRRAGIPLLLESSIKKGLDCD
                     WSDPEAKQTALQELLGQLDSLKQWLTKRPEALQEPQLEQQLQVLEQIMGQDLEPDPKS
                     PKGKPRLCIRKGVATDRRVSIEDGDMRHGRKSKSKRFSGYKRHIATALDAQIILACAV
                     TPANKPEQEAAEPLSDDIARQGYNIRELNIDRGYINAPVVEQTLERSGTVVC"
BASE COUNT          650 a          928 c          912 g          673 t
ORIGIN      
        1 ggggtgggcg accaacgcac gccgctcaag gtagtgggcc accccggctc aagccagcga
       61 gtcgcgagtg gcgcaacaca tgcgcggcaa gacgaccggc gggcacagaa ggcagccgat
      121 gttcaggggg cctgagcgcg agcctcgatg agctgaggca gggctggaat gagctttttg
      181 ccaccgagac gatcgctaag gtaactccag aaggagacgc caagcttgcg gcaggtcttt
      241 ttcaaactcg caaacgtgtc gcgacaacgg cggccatcgt cactgcgcgt gccgccgctg
      301 atcttgcggc gcttgacgta ttcgcggatg tcgccctcgc tgaggttgtt gtgcaggggc
      361 aggtcaggac ggtccaacac tagcagcagt tcgcgcttat tgcgctgcat gcgcttgagt
      421 gcgaggttga gcgaagtgaa gcacgtttca gtcgcgcaca gcgcgtcgaa tcggtcttcg
      481 atcgccagct tggccgctgg tgtcggctca agctgatagg ctttcagatc tcggtacaac
      541 tgccaaattt cggtgcggat ctggcccagg tcttggcgtt gcgagtcgtt gaagccgacc
      601 aacttggcga acacacgctc ggcgtggatc cagcacaggc cgtgcaagaa cacgttgaac
      661 tgaccggcat cgtcactaac gatggcaaga tcgcggttga aaccatgctc aagcaccgag
      721 cctagcaggg caccctcggt ggcaatgcgg acatttcgct cttgagcaat gccccgaccc
      781 ttgagcgccg actgccactg gtccttgttg gcaaatacct gtccgaccgc tggctgaagc
      841 ttagccagcg gctccttggg tagcttctgg gctgccatgt attcgagcgc ctcgtcgttg
      901 agcacgtagt cggtctgttc ggcgcgcagc agctcgaaga aattgatacg actcttgctt
      961 tctgtggtct ggaaccaggc gaacaactcg ttgccgatat gcgttgcgta gccgttcttt
     1021 ccctggtgtc gcgcccccgt gtcatccacg tggatgtgtc tcgaaacgcg cagtccaacc
     1081 cgcaggatcg cgtctttctc ttggtggaac tgctccttgc cttcggtaat gaggcgatgc
     1141 agctggcctg aggacatctg cattccccac tcctgcagct gttccaaaat cagcggttcg
     1201 gtcacctgcg cgtggtagta ctggtaaagc acgaagcagc gcgctgtcgg tccgaagtga
     1261 tcccccggac cctcgacggg caacgcaacc ggtaactcgc cagtaaccag tttgccctcg
     1321 ggcgtgagcc acttctcgac ccgatacagc gtggcctcga ctttgaccac cagctcctgc
     1381 acgacgtagt cgctgtagcc tttgaaccgg ctcccttggg gcactttctc tggcttgacc
     1441 accaccgtgt ggtctacctt ccgctctttg ccaggcttgc gtttgccctt gttctttcgc
     1501 tctttgccct cgccctcgtg cagccgcgag ggacgaatcg atggcttggg tttctggtgc
     1561 ttgagcaccg caagctcatc gcgcagcgcc tgattctctt cactcagccg atgattttgc
     1621 tccagcaacg tcgcggtctg cttctgcaac actgcgaact gcttttgcaa caccccaagc
     1681 tgctcctcca gctcagcaat ccgctcttct tgcaggtcgt gcttcacacc cccttcgatg
     1741 cctcatttgc ctccgaatgg aaagtcccaa aaaataatgg agcaagttca atcacttgcg
     1801 ccgcagcaac ctcacaccct ccgctccccg cgaccaaacc tgcacacgcc gccaccggtt
     1861 attgagaagt tacgacgatc agttacatcc tagcgtaatt gcagacaatt ttcttcgtga
     1921 tgacgctacg tgatcatatg tgatcatacg tgagcctatg cgattgtttt tcacaagcgg
     1981 cagcaattat ggtttggcag cagtcatgac gattgctcac tggaccccac gcacaacgat
     2041 ctctcggcag gagcagttct tgctcaagcg tttgcagcga actcgcaagc tgtttgcatt
     2101 tctcagactg tatcgtcacc agctgttcga tgagtctttc caagacgaat tggcgggtat
     2161 gtatcgcgac accggtgccg gcaaggatgc taaaccccca gcgctactgg cgatggcgtt
     2221 gctgttgcag ggctacctgg gtacatcaga cgccgaagta gtcgagatga cggtggttga
     2281 tttgcgttgg cagatggtcc tggaccgatt gggcgccgat gagccagcct tttcacaggg
     2341 cgcattgcag aatttccgtg agcggctgat tgccgcggat ctggaccgac ggctgctgga
     2401 gcgcacggtg gagttggcca agcaaactcg cgcgttcgat gcgaagaagt tgccgaaaag
     2461 tttgcggatt gcgattgatt ccagcccgtt ggaaggggct gggcgcgttg aagacacatt
     2521 caacttgcta gggcacgcag cacgcaaggt ggtcgaatgt gcggctgccg ctctgcaact
     2581 gagtgtcgag caagtttgcc gtcgcgccgg gatcccgttg ttgctcgaat ccagcatcaa
     2641 gaaaggactg gactgtgact ggtcagaccc cgaagctaag caaactgcac tgcaggaact
     2701 gctcgggcaa ttggactcgc tcaaacagtg gctgaccaaa cgacccgagg ctctgcagga
     2761 gccgcaattg gaacaacagc tacaggtact tgagcaaatc atggggcaag accttgagcc
     2821 cgatccgaag agtcccaagg gcaagcctcg cctatgcatt cgtaagggag ttgccaccga
     2881 ccgtcgggtg tccatcgagg acggcgacat gcgacacggg cgcaagagta aaagcaagag
     2941 gttcagcgga tacaagcgtc acatcgccac cgcgctggac gcccagatca ttttggcctg
     3001 cgccgtgacc ccagcgaaca agccagagca agaagctgct gaaccgctca gcgatgacat
     3061 cgcccggcaa ggctacaaca tccgagagtt gaatatcgac cggggttaca tcaatgcccc
     3121 cgttgtcgaa cagacactgg aacgtagcgg cacggtggtg tgc
//
LOCUS       DASRKK010000654         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1138480, whole genome shotgun sequence.
ACCESSION   DASRKK010000654 DASRKK010000000
VERSION     DASRKK010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138480"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFC15_15995"
     CDS             <1..1171
                     /locus_tag="VFC15_15995"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11710.1"
                     /translation="LCVNCHMPAKTYMVVDARRDHSFRVPRPDFSVAYGTPNACNQCH
                     KDKSNSWAADTIVKWYGPNRRQEAHFAAAIDAGRRGLPDAEKALSALIADSTKPGIAR
                     ATALSLLPQYLTPSAIPTVQAALGDSDPLVRSQAVRSLEPLSPQERVRLAAPLLPDPI
                     RSVRIEAARLLAGTPPDLLQDPQKTALERAIAELIASEMVSAERPENHMNLALLYVQM
                     GRTTEAESELQTALRLDPNFVPAMVNLADVYRAQQRDEEGQRWLEKAISVAPNAAEPI
                     HALGLLKTRQKQYPEALGLLAKAASLQPNNVRYSYVYAVALNSTGHADGAIAVLQQAH
                     QRRPADRQILMGLISFERDKGNLPSAIMYAQQLVQLVPNDPDAKAILTELQGQGR"
     gene            complement(1471..>2988)
                     /locus_tag="VFC15_16000"
     CDS             complement(1471..>2988)
                     /locus_tag="VFC15_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZM11711.1"
                     /translation="IGFKSEGILPLAAFQSAGEEVKPGDKLVVSVKGRDTDGYYELSR
                     FKTALPKDWSSLEQAFADKTTIMGTVTGVIKGGLSVDVGVRAFMPGSRSGARDAAEME
                     KLVGQEIRCRIIKLDVTDEDVVVDRRAVTEEEERSGKERRYSEVKEGETVHGTVRSLT
                     DYGAFVDIGGVDGLLHVGDIAWSRVAKPADVLSVGQEIEARVLKIDPEKRRISLGMKQ
                     IQPDPWHSAAEKYTAGERVRGTVTRVVDFGAFVELEPGVEGLIHISEMSWAKKARQAS
                     DVVKPGETVEAVVLAVSPSDRRISLGLKQALGDPWAEVTQKFGVGSVIEGPVTSLTNF
                     GAFVQLSEGVEGMIHVSEISAEKRINHPQEVLKVGQLVKTQVLAIDKERRLLRLGMKQ
                     LVPTSLDEYIAEHKEGDVVTGRLIEVSDGRARVELGEGIQGTCPIVAEGPAKEETRGE
                     SKADLSALSSMLKARWKGGAAGGASKPEAIRAGQIRSFRIAKLDAVAKKIELELA"
BASE COUNT          569 a         1005 c          818 g          596 t
ORIGIN      
        1 gctgtgcgtc aactgccaca tgccggccaa gacctacatg gtggtcgatg cccggcgcga
       61 tcacagcttc cgcgtaccgc gtccggattt ctccgtcgcc tacgggactc cgaacgcctg
      121 caatcaatgc cacaaggaca agtccaacag ttgggctgct gacaccatcg tgaagtggta
      181 cggccccaac cgccggcaag aggcacactt cgccgcagcg attgatgccg gacgtcgcgg
      241 cctaccggac gccgagaaag ctctgagcgc gctgatcgcg gactcgacga agcccgggat
      301 tgcgcgcgcg actgcgttga gcctgctgcc tcagtacctc actccttctg cgattcccac
      361 cgtgcaagct gcgctcggcg acagtgatcc actggtacgc agccaggccg tgcgctccct
      421 ggagccgctc tctccgcaag agcgggttcg gctggctgcc ccactgctcc ccgatccgat
      481 tcgttccgtg cgcatcgagg cagcgcgcct gcttgccgga actccgcccg atctcttgca
      541 agatccgcag aagacggcgc tcgagcgcgc gattgcggag ttgatcgcca gtgaaatggt
      601 ttccgccgag cgcccggaaa atcacatgaa cctggcgctg ctctatgttc agatgggacg
      661 cacgactgag gcggaaagcg aattgcaaac cgcactgcgg ctggatccaa acttcgttcc
      721 tgctatggtc aacctcgccg atgtctaccg cgcgcagcaa cgggatgagg agggtcagcg
      781 atggttggaa aaagcgatct cagttgcccc taatgcggcg gaacccatcc atgctctcgg
      841 attgctgaaa acccgccaga agcagtatcc agaagcgttg ggcctgctgg ccaaagcagc
      901 cagcctgcag ccgaataacg tccgctacag ctacgtgtat gcagtagccc tcaattccac
      961 cggccacgcc gacggcgcga ttgccgtgtt gcagcaagcc caccaaagac gtccagccga
     1021 ccgccagatt ctaatggggt tgatctcctt cgagcgcgac aaaggaaact tgccgtcggc
     1081 gatcatgtac gcgcaacagt tggtacaact ggttcccaat gatcccgatg caaaggcgat
     1141 attgacggag ttgcagggac aaggccgttg agtgggcgag ccccgctcac aacagaagcc
     1201 tcacggcagc cgggatgaca gtgtcgaatg gaatggaagc gatcatgaat tgccgggatt
     1261 gccctaagaa gaaatgtgct gccaaggcaa agagtgggcg aattgcactc cgcagtgata
     1321 gtggacgccg tcttcggtat tccagcccga tgcggcctgc acagccttca ggtcgtcgac
     1381 ctcttgcgag gcaatcggat tgcgacatcg cacccaccga gcctgcactg ggacgcctgc
     1441 attcaaaggc cgcattgcgg gcctagctta ttacgccaat tccaattcaa tcttcttcgc
     1501 gaccgcgtcc agcttcgcaa tccgaaaact gcgaatctgt ccggcacgaa tggcttctgg
     1561 cttggacgcg ccgccggcgg cgccgccctt ccagcgcgcc ttcagcatgg aactcagcgc
     1621 tgacaagtcc gccttcgatt cgccccgcgt ctcttccttc gccggacctt ccgccacaat
     1681 tgggcaggtg ccctgaatgc cttcacccaa ttccacgcgc gcccgcccgt ccgaaacctc
     1741 gatcaggcgt ccggtgacca catcgccttc tttgtgctct gcgatgtatt catccaggct
     1801 ggtgggaacc agttgcttca tccccagccg caggagccgc ctttccttat cgatcgcgag
     1861 cacctgggtc ttaaccagtt gcccaacctt cagcacttct tgcggatggt tgatgcgctt
     1921 ctcggcgctg atttcgctga cgtgaatcat cccttcgacg ccttcggata gctgcacgaa
     1981 cgcgccgaag ttggtaaggc tggtgaccgg tccttcaatc accgagccaa ccccaaactt
     2041 ctgtgtcacc tcggcccagg gatctcccag ggcctgcttt agtcccagcg agatgcggcg
     2101 gtcactcggg ctgactgcca gaaccaccgc ttcaacggtc tcgcccggct tgaccacgtc
     2161 gcttgcctgc ctcgccttct tggcccacga catctcggaa atgtggatca agccttcaac
     2221 cccgggctca agttctacaa aggctccgaa gtcgacgacg cgcgtcacag tgccccgcac
     2281 gcgctcgcct gccgtatatt tctcggccgc cgaatgccag ggatcgggct gaatctgttt
     2341 catgcccagc gaaatgcgcc gcttctcagg atcgatcttg agaactctgg cttcgatctc
     2401 ctgccccacc gacagcacgt cagcaggttt cgcaacgcgg ctccaagcga tgtcgccgac
     2461 gtgcagcagt ccgtccactc cgcctatatc gacgaaagcg ccgtaatcgg tgagactgcg
     2521 gaccgtgcca tgcaccgtct cgccttcctt gacctcggaa taccggcgct ccttcccgct
     2581 gcgttcttcc tcttccgtca ccgcacggcg gtccaccacc acgtcttcgt cggtcacgtc
     2641 gagcttgata atccggcagc ggatctcctg ccccaccaac ttctccattt cggcggcatc
     2701 gcgtgctcca ctgcgcgagc ctggcatgaa cgctcgtact cccacatcca cgctcaagcc
     2761 gcctttgatc acgcccgtca ccgtgcccat gatcgtcgtc ttgtcggcga aggcctgttc
     2821 cagcgatgac cagtccttgg gcagggcggt cttgaagcgg gaaagctcgt aatagccgtc
     2881 ggtgtcgcgg cccttcaccg aaacgaccag tttgtcgccg ggtttcactt cttcgccggc
     2941 gctctggaac gctgccagcg gcaggatgcc ttcgctcttg aaaccgat
//
LOCUS       DASRKK010000655        18480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1041194, whole genome shotgun sequence.
ACCESSION   DASRKK010000655 DASRKK010000000
VERSION     DASRKK010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18480
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041194"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..753
                     /locus_tag="VFC15_16005"
     CDS             103..753
                     /locus_tag="VFC15_16005"
                     /inference="COORDINATES: protein motif:HMM:NF044290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11712.1"
                     /translation="MRVNTNSFPDIPNGILTLNGAAVAIYIIVQLIASVDSGRDSRLR
                     LVEAQRNSLAPPDNVQYAVDLAAATLEDYLNRNLSQVKWIFNVAVFVMFCGFAVVCYG
                     ASIAMGGRIEASLAVSASGIITQFISVTFMVIYRSTMERANSNVTILARINSIGMAVQ
                     VLEKMPDSAAELKNTIRADIVRTLLAESEDTQISPARPRKQVAKPHQPESASAPAE"
     gene            complement(826..2124)
                     /locus_tag="VFC15_16010"
     CDS             complement(826..2124)
                     /locus_tag="VFC15_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11713.1"
                     /translation="MIDPEIGFTSDVIRDPSRFVGRTDLIRDCIKSLNTPSGLIAVYG
                     KRGVGKSSLLRQIQQMSLGDYTLAKNAGLSHEIPARPRKYLTVYYVCDALIENGGDLL
                     SRMCNDQQAEDGLLRLVPQDGKEVVEFTRSKEVFGGADLKVVNWGAKGIESSKYARVV
                     PGDTVQTFRNFVDAVITHQVKSRMKRDGLLILLDEFDVIKDKQGLGSLIKSLTSHELK
                     FGICGIGQDLNDIVEDHASVERLLEEGAIHVQPMPKEEIDEIFYRAEQLFAGKLKFQR
                     EAIVKISELSQGYPYFAQLIGKECVNQVNRIGSQTIDSSVLQRVLDDVKSGRAFPTLE
                     SAYQRAIGSSKDREVLLHLLADQPEENAMFNEEVGRVVLKNARGDADDLNVQYVDQLV
                     PRLVDPKFGPVLRRTSERQGVYEFVNPVLRVYIKLRNFSF"
     gene            2279..2545
                     /gene="rpsO"
                     /locus_tag="VFC15_16015"
     CDS             2279..2545
                     /gene="rpsO"
                     /locus_tag="VFC15_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZM11714.1"
                     /translation="MLAREKKTAVIDQYRTHDTDTGSPEVQIAILSERIGELTEHFKT
                     HKKDHASRRGLLMMVSRRRGLLDYLRKSDTERYKVVIQKLGIRK"
     gene            2891..5221
                     /gene="pnp"
                     /locus_tag="VFC15_16020"
     CDS             2891..5221
                     /gene="pnp"
                     /locus_tag="VFC15_16020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZM11715.1"
                     /translation="MKHEVTVELHGGKSIHFETGKLAKQAHGSCIVRIGENVVLATAC
                     ANPDPREGIDFFPLTVDYREYTYAAGRIPGGFIKREGRPSEREILTSRQIDRPIRPLF
                     PEGFRCETQVIAMVLSADTSNDPDVAGINGASVALSVSDIPFHGPIGAVRVGQVNGEF
                     VINPSYDEQREGKLNLMVVGTMDGIVMIESGAKEVSEETVVEGIEFGHQEIKKICAAI
                     NDLVKKVGKVKRTVEPVITDEAYLGELRSKFGAKLIDAVNTEKYGKNESYGLIKKLKT
                     EVKETVPEDDEAGRKKVGKYFDLLREEIFREQVIKEKRRPDGRKFDQIRDIWIEVGVL
                     PRTHGSAIFTRGETQALVTTTLGTGEDMQRLEAFEGETRKRFMLHYNFPPFSVGEVAF
                     LRGAGRREIGHGALAERALFAVLPDQTDWPYAMRVVSDILESNGSSSMATVCGASLSL
                     MDAGVPLKASVAGVAMGLVKEGDDYAILTDIAGAEDHYGDMDFKVAGTREGITALQMD
                     IKVMGITAQIMREALEQARHGRLYILDKMNEVIREPRTTISEFAPRFYTLQISTDKIR
                     DLIGPGGKVIRGIIEQTGVKIDVEDSGKVNVSSSDQVAAAKALQMIGDITATAEVGKT
                     YLGKVTRLADFGAFVEIIPGTEGLLHISEVAEHRINDVRDELKEGDQILVKVLAVEGN
                     RIKLSRKAILKEQRAKMGGGEGAPEGAEGSEGGEGAPVPVAFEGGHESADEPNFNREG
                     SRPPRRDGGGDRGPRRGGPGGGRGGDRGGRGRGPRR"
     gene            5357..6025
                     /locus_tag="VFC15_16025"
     CDS             5357..6025
                     /locus_tag="VFC15_16025"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZM11716.1"
                     /translation="MPCLLYCVSHLESEAVRDVVGVCDEPLRGLESLGLRVYWSEIAD
                     PEAALAEGAARKGAELKYQQVLRQIVAQTTPIPLPFPVVLADVEAVEKHIGEEREFFG
                     EALNRLGDTVQYEMIASWTADEQADLATPVSGREYLKRRQESEERIAAIDAKLKKVGA
                     GCVRGWRARQDRRKHHWFALVERQDRERFVGALRSAGPSEGVRLRLSGPWPPSEFVKA
                     SDAS"
     gene            6252..9584
                     /locus_tag="VFC15_16030"
     CDS             6252..9584
                     /locus_tag="VFC15_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZM11717.1"
                     /translation="MQKFWSSSICLLLLISSLWAQQARPKEPGRAAVTQEAKPAPTTK
                     PPASEPADYSQEAFVTELYRESFRFENDGTGREQIDARIKINSDSGVQALGQLKVGYS
                     ALSDKLEVVYVRVIKPDGTVITAQESAIQDLTLPDAPVYTDYHQKHITVPSLRPGDVL
                     EYQFVRTIVNPLTPGQFSTSYDFRDRGIVLDEQLEINVPKSRQIKLKSEPGAEPKITE
                     DGDRRIYRWKHSHLEDEEDTAKKKRKPVRRHDEDDVPSVQLTTFESWQQLGNWYASLE
                     KDRRQPNDAIKAKADELVQGKTTDTEKVKALYDFVSRDFRYVSLSFGLGRYQPHAASE
                     VLANGYGDCKDKNTLLAALLASQGFESTSVLIGSQHKLDPDIPSPSQFDHVITRVPVD
                     GQEIWLDSTNGVGPFRMLSFPLRDKQALAMPPGGTPGLVRTPAGLPFDSFDLSRIEGA
                     LNDTGKLTVHFNAAARGDAELGMRFGLRQIPNNRWKSVFEMMLSRSPMKGGEITNLKV
                     GDPANTEKPIEVDFDIAVSNYFDWSAPDPKLPLPVIGISLPPDFDDDSKNPKPIKLGA
                     MTEASTEVKIAIPAKYGVRLPIGVDVKRDYAEYHSSYKFEDGQLSAVRKLRTLLSEIP
                     YERREEYAAFRRTIDADQAQNITLDNKSPGAAGLGAEQSPDDLYDSALQAMENNNYAL
                     VIDLFQRVLKADPKHKGVWNNLGRAYLASNQYDRAADAFKKQLEINPYDEFAYNNLGR
                     TYEAMQRYDEAIAQFQKQIEINPLDPYAHSNLGLLYSKLKRWNEAVPELEKGVALQDK
                     NPLLHISLGQAYIATGQTEKGMASFDRAIALSPNPVVWNNIAYSLAEQNVQLDRASRY
                     SDAAINAIETQLRDVNLDSLRLQDIAVANLLYNVWDTKGWVEFQRGNLDGAERYIRAA
                     WEATGSGNVCEHLGEIYEKRGNKDEAIHYYVLALVGQSPSDDARSHLTALGVTGDLDS
                     RIEKGRAEMEALRTRKLDASGKGTGDFFALVSPTKNDQIKFVSGDAEIKGLADVVKAT
                     NLGVKFPDPASVRALRRGTVKCGTLPPEPVIKGKAASKKGANGKAPVETTGKKEPSAK
                     PELLPGPCTVELLFSDGVRSVD"
     gene            9624..10160
                     /locus_tag="VFC15_16035"
     CDS             9624..10160
                     /locus_tag="VFC15_16035"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HZM11718.1"
                     /translation="MEDLRYPIGKFEWVPAKNEEQMAQRRAKYIDVLAKLPENFSHAV
                     TDLTSKQLDTPYRPEGWTVRQLIHHVPDSHANAYIRFKLALTEYEPAIKPYKEDMWAK
                     LPDTANTPIDVSLQLLDALHVRWATLLRAMDSSDFARTLRHPELGVLNLNRMLALYAW
                     HSAHHTAHITKLRERMGW"
     gene            10472..10843
                     /locus_tag="VFC15_16040"
     CDS             10472..10843
                     /locus_tag="VFC15_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11719.1"
                     /translation="MTRFPVRTGLLVLALMLSLSAFAKPKTDTITLFDETTVSGTNLA
                     AGQYTVKYDVNGSTAQVKFLKGNKEVATANGQVKNLTKKADSTQVVLNTEGNARSIAE
                     IDFGGKDTAISFESSRTTAGK"
     gene            complement(10976..14830)
                     /gene="smc"
                     /locus_tag="VFC15_16045"
     CDS             complement(10976..14830)
                     /gene="smc"
                     /locus_tag="VFC15_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZM11720.1"
                     /translation="MLKLKKLQILGFKSFCDRTELKFQGDGIAAIVGPNGCGKSNISD
                     AISWVLGEQSARSLRGVNMQDVIFAGTRERKPTGMAEVTLTLIDPGVYGDAGDPEIDI
                     QDDMPEEDWDESSIRAAKADDAERYTEEVRPGQGDDEAGASVEGQASTQPEATAAETT
                     TGTHVVLKIRRRKFVRTFKAGEIAVTRRLFRSGESEYLLNGKLCRLRDIQDIFMGTGL
                     GPESYAIIEQGRIGQILSSKPTDRRAIIEEAAGITKFKTRKRLAEARLEDAKLNLARI
                     NDIFDEVTRQMNSLKRQASKAERYARLRDEMRAKLRVVLASKFTQLDAESASLEAQLT
                     EIGEEMRVRGEALATMEAEHGERTQRGYAIEREGNENRERLNAVVLEIDRAIARRRTN
                     EERCAELDARSAGAQAEVGSTQEQLRRLQQELEANREILESANADVAQAQNELQQRQQ
                     EASSAAATLMEVERQQELRRAAILQAVSAASSVRNRMTQAEERVAALDREAHRLEEET
                     SIANQQLENFGGQRGQLGLQFESASQQVAALTAQIDDSRQNLEQKRSAETNGKQHLDS
                     LRAEYASLIGKKGSLEAVITEHGYSTESVRRLFKSGAMSGGMAPVGVLADFLEVDDRY
                     EHVVDDFLRDELNYVVVKSWDAADEGLRLLRSDVDGRATFLVHPEDSQAKFSFLSEEG
                     SYAGAQRDTVVPLKHCIRVLDGFGKSLEVILPKLGNGYIVPDPNVGRELALQNPDAFF
                     LSQSGECFHNVTVTGGKQRSEGPLAMKRELRDVLRQAADVEGAMAAEQARVAVLTREI
                     SEITAMAQRLEEDKREAERQAMTSGHTLQQLESEMARVRERLSTYGRETQRLAAECGE
                     CEVAIAAHRTELATHESQKIALEAEMQVAQSKLDELRQQRDASAQHASDSRARLATLE
                     ERRRGSAVTVERLEAMMTEVSSRLDKLKAQIDSSAAEKQQRETENTRLAEQQIDWTAE
                     RETAQHRDRELQEELQAVRVRLGELEERLKNLRHELDTARDRRGELAAEQARLHSDAQ
                     HMAETCIQELSLPREELLSDEALTRLAGEDLTTEESLYKEMRTRLENMGPVNMMALEE
                     YQETAQRHEFLETQRLDLLQSIENTQNTIKEIDTISRQKFEEAFNHINENFARTFSKL
                     FGGGQAFMRLTDLENSMESGIDVVASPPGKKLQNVLLLSGGEKALTALALLVGIFQYQ
                     PSPFCILDEVDAPLDEANIGRFTELVRDMSGQTQFIIITHSKKTMGIAPVMYGVTMQE
                     PGVSKLVSVRFHEEPARMAATA"
     gene            complement(15449..16729)
                     /locus_tag="VFC15_16050"
     CDS             complement(15449..16729)
                     /locus_tag="VFC15_16050"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZM11721.1"
                     /translation="MKLLRATQYASVVSFALVLSLGVGFAQTLTGRPVAAATVSLSEN
                     PYTEQPVRAFPRLNLWDGEVLTYLGVFSPDAVFHTTSRLTGVTGRSPGEGVPSADFRN
                     HGQPLSDAPASMLLSNEREVESLEPPAHAEAIAHVPARVAEIRNRFITYAYGRPSVLS
                     APRHIATDSRHRLIISDPDASAVHVLDPSGRTSFRIMTGPGRRLRQPAGVAVDADDNI
                     YVADSQRGMLVVFDRYGNFARYIGNFKGENEYESPHGIAIDQKAGRLYLVDSPRNLVF
                     VLDLMGKVLRRLGKYHDGTGVGEFEDPTDIAVNHDHVYVLDSSGTRVQIMDSQYAFRG
                     DFNLPRDRSPLTSRENGLAADQRGNVFVTSFSGSRIRVYNQVGRPLASFGLPGQRVGE
                     FAGPGGLCIDSFNHLFVADSGNGRVQLFQLQELP"
     gene            complement(17108..18073)
                     /locus_tag="VFC15_16055"
     CDS             complement(17108..18073)
                     /locus_tag="VFC15_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11722.1"
                     /translation="MKRFVLRSASAVFALALMAQMVWAAPYSFQSVISPGDPAFTQLL
                     GINNSSTIAGYFGDGTAVANNGFTLTLPSSYTAENFPGAAQTQVIGINNTGWNDGFYT
                     DGGGVTHGFTYNGSFTNVDAPGTAFNQLLGVNDGLTAVGYSSLDPSGMTLQRSFMVAG
                     TTFTYLDGFLPGGTQNNQAVGINNAGWVVGFYADSSGALHGYLFNGTSATTLDFLGGM
                     GTQAFGINNHGEIVGDYTDSAGTMHGFTYLGGVWQTVDDPLAVPGSTVINGVNDLGQL
                     VGFYADANDNVIGFVATPTPEPGTLVLLGSGILAGLGVLRRKLLT"
BASE COUNT         4060 a         5127 c         5511 g         3782 t
ORIGIN      
        1 cccctacagt aattgttgat ggcgtagatg tctcactatt gttgacacag agggatcggg
       61 acgtttgcaa tcacggggta catgctattg gagagtttcc atatgcgcgt taatacgaac
      121 agtttcccgg atattccgaa cgggatcctt acgctgaatg gggccgccgt ggcaatttac
      181 atcatcgtac agttgatcgc gagtgtagat tctggaaggg acagtcggtt aaggcttgtt
      241 gaagcgcaac ggaattccct agccccccct gacaatgttc agtacgccgt cgacctggca
      301 gctgcgacac tcgaagatta cttgaaccgc aatctttccc aggtcaaatg gatctttaac
      361 gtcgcagtgt tcgtcatgtt ttgtggcttt gcggtggtct gttacggagc gagcatagct
      421 atggggggac gaatcgaggc aagtcttgcc gttagcgcat ccgggattat tacccagttt
      481 atcagcgtaa cgtttatggt gatttatcgt tccactatgg aacgggcaaa ttcaaacgta
      541 acgatccttg ctcgaatcaa tagcatcggg atggcagttc aggtccttga aaagatgccg
      601 gattcggctg cagagttaaa aaacactatt cgcgccgaca tagtgcgaac gcttcttgcg
      661 gaatcggagg atacgcaaat ctcacccgct cgcccgcgca aacaggtcgc caagccacac
      721 cagccagagt ctgcttcagc gcctgcggag tagaggttag tgacccgcgg gttatggaag
      781 tgggcgcgaa ggggagcccg cgcatccgaa gcggcagcgg agtgttcaga agctgaaatt
      841 tcggagctta atgtaaacgc gcaagaccgg attcacaaac tcatacacgc cttgtctctc
      901 agatgttcgt cgcagaacgg ggccgaactt gggatctacg agtcgcggaa caagttggtc
      961 gacgtactga acatttaggt cgtcggcatc gccacgagca tttttcagga cgactcgacc
     1021 gacttcttcg ttgaacatgg cattttcttc tggctgatca gcgagtagat gtagcagaac
     1081 ttcgcgatcc ttagaactcc cgattgcgcg ttggtacgca gactcgagcg tcggaaatgc
     1141 gcgtcccgac ttaacgtcgt caagaactcg ctgcagaacg gaactgtcga ttgtctggct
     1201 cccgatgcga ttcacttggt tgacacattc tttgccaatc agttgcgcaa agtaagggta
     1261 cccttgagac agttctgaga tcttcacaat cgcttcccgc tgaaatttga gcttaccggc
     1321 gaagagttgc tcagcccgat agaagatctc atcgatttct tccttaggca tcggctgaac
     1381 gtgaattgcg ccctcttcta ggagtcgctc taccgatgca tgatcctcga cgatgtcatt
     1441 caaatcttgc ccgattccgc agataccaaa tttgagttcg tggctcgtca gcgacttaat
     1501 gagggaccct agtccttgct tgtccttgat aacgtcgaat tcatcgagga gaattaggag
     1561 gccgtcccgt ttcatccggg acttgacttg gtgggtaatt acggcgtcaa cgaaatttcg
     1621 gaaagtctgc acagtgtcac cgggcaccac gcgagcgtat ttagaactct caatgccctt
     1681 tgcgccccag ttgactactt ttaagtcagc accgccgaag acctcttttg aacgcgtaaa
     1741 ttcgacgacc tctttcccat cttgtggcac caatcgaagc aaaccatctt ccgcctgttg
     1801 gtcgttgcac atgcggctaa gcaagtcgcc gccattctcg atcagcgcgt cgcaaacgta
     1861 ataaacggtg aggtactttc gaggtcgcgc tggaatctcg tgagacaaac cagcattctt
     1921 ggcaagggtg taatcaccaa gggacatctg ttgaatctgg cggagcaagg acgatttgcc
     1981 gacgcctcgt ttgccgtaga cagcgatcag gccagagggt gtgttcagcg actttatgca
     2041 atcacggatt aagtcagttc gtcccacgaa tcgggacggg tctcgaatga cgtcggaggt
     2101 aaaaccaatt tctgggtcga tcataggtat gtgaaagaga tccgaccaaa aagtaccaca
     2161 cagaccccgt tcttgcagtg acacggacat ggtcactcag ttggcggtgg gcgcggatgc
     2221 gtgttattct ttatgacgta acaacatacg attaaagagc aggagtgcac ccgaaacagt
     2281 gttagcccga gagaaaaaaa ccgccgtaat tgaccagtac cgtacccacg acacggatac
     2341 cgggtcgcca gaagtgcaga tcgcgatcct gagtgagcgg attggtgaac tgacggagca
     2401 ttttaagacg cataagaagg accatgcctc ccggcgcggt cttctcatga tggtcagcag
     2461 gcgacgcggc ttgctggact acctcagaaa gtccgatacc gaacgctaca aggttgtgat
     2521 tcagaaactg ggaatccgca aataagcagc ttgccagcca ctggagtacc gctttaccag
     2581 cgtgatgtgc gtcgaatcaa cccagcaggc gccggtgtgg cgcgggccgg gctcccccga
     2641 gatagtggtc tatttcggga agaaacctat tttcgagaca tggcggacgc gagagagcgt
     2701 ccgcagcctc ggtttcagcc actctcggct gaccagtatt ccatcctccc catcccgcag
     2761 ccgccggatc gcgttctcgc gtcacgtttg cagtgcccga gtttcaggat tccgacccct
     2821 acggggagcg ccgagcctgg acgacgtcta ctgggcgacc attgtcggat gcattcgaaa
     2881 aggacaataa atgaaacacg aagtaacagt tgagttgcat ggcggaaagt cgattcactt
     2941 tgaaaccgga aaattagcca agcaggcaca cggttcctgc atcgtgcgca ttggcgaaaa
     3001 cgtggttctc gccacggctt gcgccaaccc cgatccccgc gaaggcatcg acttcttccc
     3061 actcacggtg gattaccgcg agtacaccta cgccgccgga cgcattccgg gcgggttcat
     3121 caagcgcgaa gggcgtccca gcgagcgcga gattcttacc agccggcaga tcgatcgccc
     3181 gattcggcca ctgttccccg aaggattccg ctgcgagacg caggtcatcg caatggtgct
     3241 gtcggcggat acgagcaacg atcctgatgt ggccggaatt aacggcgcgt cggtggcgtt
     3301 gagtgtgtcg gacattccgt tccacggtcc catcggtgca gtgcgcgttg gacaagtgaa
     3361 cggcgagttc gtgattaacc cgagctacga tgagcagcgc gaaggcaaac tcaacctgat
     3421 ggtcgtcggt accatggacg gcatcgtgat gatcgagtcg ggtgcgaagg aagtttcgga
     3481 agagacggtc gtcgagggca ttgagttcgg gcaccaggaa atcaagaaga tctgcgcagc
     3541 gattaacgat ctggtgaaaa aggttggcaa ggtgaagcgc acggtcgagc cggtgatcac
     3601 cgatgaagcg tatctcggtg aactgcgctc gaagttcggt gcgaagctga tcgatgcggt
     3661 gaataccgag aagtatggaa agaatgaaag ctacgggctg atcaagaagc tgaagaccga
     3721 agtgaaagaa acggttccgg aagacgacga agcgggccgg aagaaggtcg gcaagtactt
     3781 cgacctgctg cgcgaagaga tcttccgcga gcaggtgatc aaggagaaac ggcgtcctga
     3841 tggccgcaag ttcgaccaga tccgcgacat ctggatcgag gttggcgtgc tgccacgtac
     3901 gcacgggtcg gcgatcttta cgcgtggcga aactcaggca ctggtgacca ccacgctggg
     3961 cacgggcgaa gacatgcagc ggctggaagc cttcgaaggc gaaaccagaa agcgtttcat
     4021 gctgcactac aacttcccgc cgttctcggt gggggaagtg gcgttcctgc gtggcgcggg
     4081 acggcgcgag attggccatg gcgcattggc cgagcgggcg ctgttcgcgg tgctgccgga
     4141 ccagacagat tggccgtacg cgatgcgcgt ggtctcggac attctggagt cgaatggctc
     4201 gtcgtcgatg gcgacggttt gtggcgcttc gctcagcttg atggatgccg gcgttccgtt
     4261 gaaagcctcg gtggccggag tcgcgatggg gttggttaag gaaggcgacg actatgccat
     4321 cctgaccgac attgcgggcg ccgaagatca ttacggcgac atggacttca aggttgccgg
     4381 tacgcgcgaa ggcattacgg cgctgcagat ggacatcaag gtgatgggca tcacggcgca
     4441 gatcatgcgg gaggcgctgg agcaggcacg ccatggccgg ctctacattc tggacaagat
     4501 gaacgaagtg attcgcgaac cgcgcaccac gatcagcgaa tttgcgccgc gcttctacac
     4561 gctgcagatt tcgactgaca agattcgcga cctgatcggg ccgggaggca aggtcattcg
     4621 cggcatcatc gagcagacag gcgtgaagat cgacgtggaa gacagcggca aggttaatgt
     4681 gtcttcgagc gatcaggtcg cagccgccaa ggcgttgcag atgatcggcg acatcaccgc
     4741 gaccgccgaa gtgggcaaga cgtatctggg caaagtgacg cgcctggccg actttggagc
     4801 gttcgtcgag atcattccgg gaaccgaagg cctgctgcac atcagcgaag tggcggagca
     4861 tcgcatcaac gatgttcgcg atgaactgaa ggaaggcgat caaatcctgg tgaaggtact
     4921 agccgttgaa ggcaaccgca tcaaactgtc tcgcaaggcc atcctgaaag agcagcgggc
     4981 caagatgggt ggcggcgaag gtgcgcctga gggagcggaa ggctcggaag gcggcgaggg
     5041 tgctccggtt ccggtggcgt tcgaaggcgg acatgaatcc gcggacgagc cgaacttcaa
     5101 tcgtgaaggc agccgtccgc cacgtcgtga tggcggcggc gatcgcggac ctcgccgggg
     5161 tggcccgggt ggaggacgcg gcggtgaccg tggtggccgt ggacgtggcc cgcgccggta
     5221 gaagagcagt tgtcagtggc cagttgtcgg tcgtcagaaa ccgacttctg tccatagttg
     5281 gcgaattcgt caagacgagg ggagggcaga gatgctctcc catttttccg tcagtcatag
     5341 caaagcgaaa cactgaatgc cctgtcttct ttattgcgtc tcacacctgg agtccgaagc
     5401 cgttcgtgat gtcgtagggg tgtgcgacga gcctctgcgc gggctggagt cgttgggcct
     5461 gcgggtgtac tggagcgaga tcgcggatcc cgaagcggct ctagctgaag gtgcagccag
     5521 aaaaggtgcg gagctgaagt atcagcaagt gttgcgtcag attgtggcgc agacgacgcc
     5581 gataccgttg ccgtttccgg ttgtgctcgc cgatgtcgag gccgtggaga agcacattgg
     5641 ggaagagcgc gaatttttcg gcgaggcttt gaaccggctg ggcgataccg tgcaatacga
     5701 gatgatcgcc agttggacgg cggatgagca ggccgacttg gcgactccag tgagcgggcg
     5761 ggagtatctc aaacgccggc aggaatctga agaacgaatt gccgcgatcg atgccaagct
     5821 gaagaaggtg ggggcgggct gcgttcgcgg gtggcgcgcc cggcaggacc gcagaaaaca
     5881 ccattggttc gcgctggtgg aacgtcagga tcgggagcgt tttgtcggcg cgctgcgcag
     5941 cgccggtcca tcggaagggg tacgcttgcg gttgagtggt ccgtggccgc cgagcgagtt
     6001 tgtgaaggcg tctgacgcaa gttgaagagt gcctcgtgag tgaggagaaa gcagattcct
     6061 cgcgggctga atcccgctcg gaatgacaaa caagaaggac ttgtacggct gcacggctga
     6121 agccatgccc tttcaaaact cgggcagacg ggtgctgtca aagctgcgta caccactact
     6181 ctaactgctc gggcgcgccg gattcggttc ctcccgtccc gagaatgggt tctctgtcaa
     6241 actataagat catgcaaaaa ttctggtctt cgtccatctg tttattactt ctgatttctt
     6301 cgctatgggc gcagcaagcg cgcccgaaag aacctggccg cgcggccgtc acgcaggagg
     6361 ctaagcctgc tcccacgaca aagccgcccg cgagtgaacc cgccgactac tcacaagaag
     6421 cgtttgtaac cgaactctac cgcgagtcgt ttcgtttcga gaacgacgga actggacgcg
     6481 aacagatcga cgcgagaatc aagatcaaca gcgacagcgg agtgcaggcg ctgggacaac
     6541 tcaaggtcgg atacagcgcg ctcagcgaca agctcgaagt ggtttatgtc cgcgtcatca
     6601 agccggatgg gacggtgatc accgcgcagg agtcggcgat tcaggacctc acccttcccg
     6661 acgctccggt ttataccgac tatcatcaga agcacattac ggtgccgtcg ctgcggcccg
     6721 gcgacgtgct ggagtaccag tttgtccgca cgatcgtgaa cccgctcacg cccggacagt
     6781 tctccaccag ctacgacttc agggaccgcg gcatcgtgct cgacgagcaa ctcgagatta
     6841 acgttccgaa gtcgcggcag atcaagctga agagcgaacc gggagctgaa ccaaagatca
     6901 ccgaagatgg agaccgccgc atttaccgct ggaagcactc gcatctggag gacgaagaag
     6961 atactgcgaa gaagaagaga aagcccgtcc gccggcacga tgaggacgat gtcccgtcgg
     7021 tgcaactgac tactttcgaa agctggcagc agcttggcaa ctggtacgcg agcttggaaa
     7081 aggaccgccg ccagcccaac gacgcgatca aagccaaggc cgacgaactc gtacaaggca
     7141 agactaccga tacggaaaaa gtgaaggcac tctacgactt cgtgtcgcga gacttccggt
     7201 atgtcagtct ttcgttcggg ctggggcgct atcagcccca tgccgcctcc gaggtcctgg
     7261 cgaacgggta cggcgactgc aaggacaaga acacgctgct ggctgcgctt ctggcgtcac
     7321 agggttttga atcgacctcg gtgttgattg gttcgcaaca taaactcgat cccgacattc
     7381 catcaccttc gcaattcgac cacgtgatta cacgcgtgcc cgtggatggc caggagattt
     7441 ggctcgacag cactaacgga gtgggaccgt tccggatgct gtcgttcccc ctgcgggaca
     7501 agcaagcgct tgccatgcct cctgggggca ctcctggcct ggtgcgaact ccggcgggac
     7561 ttcccttcga tagcttcgac ctcagtcgca tcgaaggcgc attaaacgac accggcaagc
     7621 tcaccgttca tttcaatgcc gccgctcgtg gcgacgcgga gctgggcatg cgattcggtc
     7681 tgcgccagat tcccaataac agatggaaga gcgtcttcga gatgatgctg agccgctcgc
     7741 cgatgaaggg cggcgagatc acgaacctta aagtgggcga ccctgccaat accgagaagc
     7801 ccatcgaagt cgatttcgac attgcggtca gcaactattt cgactggtca gcgcccgatc
     7861 ccaagctgcc gctgccggtg atcgggattt ctcttccacc cgattttgac gacgacagca
     7921 agaatcccaa gccgattaag ttgggcgcga tgactgaggc ctcgactgaa gtgaagattg
     7981 ccattcctgc gaaatatggg gtgcgcctgc ccatcggggt cgatgtgaag cgcgactatg
     8041 cggaatatca ctccagctac aagttcgagg acggccaact cagcgcggtt cgcaagctgc
     8101 gaactctgct tagcgaaatt ccgtacgagc ggcgcgagga gtatgcagcg tttcgtcgaa
     8161 ccatcgacgc cgatcaggcg caaaatatca cgctcgacaa caagtctccc ggggctgcag
     8221 gccttggcgc cgaacaatcg ccagacgacc tttacgactc cgcgctgcag gccatggaga
     8281 acaataacta tgcgctcgtc atcgatttat tccagcgggt gctgaaggcg gacccaaagc
     8341 acaagggggt gtggaacaat ctgggacgcg cctatctggc ttctaaccag tacgaccggg
     8401 cggcggacgc tttcaagaag caactggaga tcaacccgta cgacgaattt gcttacaaca
     8461 atcttggacg cacgtacgag gcaatgcagc gctacgacga ggctatcgcg cagtttcaga
     8521 agcagatcga gattaatccg ctcgaccctt acgcgcacag caatcttggc ctgctctaca
     8581 gcaagctcaa gcgatggaat gaggcggttc ccgagttgga aaaaggtgtc gcgcttcagg
     8641 acaagaaccc gttattgcac atctcgctcg gccaagccta catcgcgacc ggtcagacgg
     8701 agaagggtat ggcgagcttc gaccgcgcca tcgcgctgtc tcccaatcca gtcgtctgga
     8761 acaacatcgc ctactcgctg gctgaacaga atgtgcaact ggatcgcgcc agccggtatt
     8821 ccgatgcggc cattaacgcc attgagacgc aacttcgcga tgtcaatctg gatagcctgc
     8881 gcctgcagga catcgcggtc gccaacctgt tgtacaacgt gtgggacacc aagggctggg
     8941 tggagttcca gcgcggcaat ctcgacggcg ccgagcgcta tattcgcgcc gcatgggaag
     9001 ccacaggcag tggcaatgtc tgcgagcacc tcggagagat ctacgagaaa cgcggcaaca
     9061 aggacgaggc cattcattac tatgtgctgg cgctggtcgg ccagtcgccc agcgatgacg
     9121 cccgaagtca tctgactgca ctcggtgtga ccggcgatct tgacagccgg attgaaaagg
     9181 ggcgcgccga gatggaggcc ctgcggacgc gcaagttaga cgcgtccgga aagggaactg
     9241 gtgatttctt cgcgctggtg tcgccaacca agaacgacca gatcaagttc gtcagcggag
     9301 acgcggagat caaagggctc gcggacgtgg tgaaggccac caacctcggc gtcaaatttc
     9361 ccgatccggc gtcggtccgc gcgttgcgtc gcggcacggt gaagtgcgga acactgccac
     9421 ccgaacctgt catcaagggg aaggctgcca gcaagaaggg tgcaaacggg aaggcgccgg
     9481 ttgaaacgac ggggaagaaa gagccttccg cgaaacccga gttgttgccg ggaccgtgca
     9541 cggtggagtt gttattctcc gatggggtgc gatcggtcga ttagaataat tcggttctca
     9601 tctgcgtatt gaaggggatg gcgatggaag acttgcgata cccgataggg aagttcgaat
     9661 gggttcctgc gaagaacgaa gagcagatgg cgcagcgtcg agccaagtat atcgacgtgc
     9721 tcgcgaaact gccggagaat ttcagccatg cggttacaga cctgacttct aagcagctcg
     9781 atacgcccta caggccggag ggctggaccg ttcggcagtt aattcaccac gtgcccgaca
     9841 gccacgccaa cgcctatatc cggttcaaac tggcgctcac cgaatacgag cccgctatca
     9901 aaccttataa agaagatatg tgggcgaagt tgcctgatac agccaacact cccatcgatg
     9961 tttctctgca gttactcgat gcgttgcacg tgcggtgggc gactcttctc cgcgccatgg
    10021 actcctcgga ctttgcccgt accctgcggc atcccgagtt gggtgtgctg aatctgaacc
    10081 gcatgctcgc gctctatgca tggcactcgg cccaccacac tgcgcacatc acgaagttgc
    10141 gcgaacgtat gggatggtag tggtcgctca ccggacttgg tgagtagcgg ccgcaaacaa
    10201 aatccaagct gtgagtctgc cgcgccaatt cattcctgga tcggtttgtc ccggaaagcc
    10261 gcgtactcat cccacgcaaa actgcgacga ttcgtcgaat tccgtgacga taacttggca
    10321 ttcggccttg agatggggtt ttcttagtgc caatcgcatc ttccgctcgt aaatctgctt
    10381 cgcttccgta aagcaactgt cgccgtcaat acacccggtc acagcgagcc gcaactcaag
    10441 gctgctgact aatctcacga ggagttcccc aatgacacga tttccagtac gcacaggttt
    10501 gttggttcta gcgctgatgc tgtcgctcag cgcatttgcg aaaccgaaga ccgataccat
    10561 taccctattc gacgaaacga cggtcagcgg aacgaacctc gctgccggac agtacaccgt
    10621 gaaatacgac gtgaacggta gcactgcgca ggtgaagttt ctgaaaggca ataaggaagt
    10681 tgctactgcg aacggtcagg tgaagaacct caccaagaaa gcagattcga cccaggttgt
    10741 cctgaacacc gaaggcaacg cgcgcagcat cgccgaaatt gattttggcg gcaaagatac
    10801 agcgatcagc tttgaatcga gcagaacgac ggcagggaag tgaccggggc ctgaagaggt
    10861 cgtggaaatg gccggaaaaa caaattcctc gtgggcccaa acccacgagg aatgaaaaca
    10921 accgggaacc cgtacgccct aactgaagct gctccccctt gaaaacatcg caacgttacg
    10981 cggtcgccgc catccgggcc ggctcttcat ggaagcgcac cgacaccagc tttgagacgc
    11041 ctggttcctg catggtgacg ccgtacatga ctggcgcgat gcccatggtc ttcttgctgt
    11101 gggtgatgat gatgaactgg gtttggccgc tcatgtcgcg aaccagttcg gtgaaccgtc
    11161 cgatgtttgc ctcgtccaac ggggcgtcaa cctcatccag aatgcagaac ggactgggct
    11221 gatactggaa gattccaacc agcaacgcta gcgccgtgag cgccttctcg ccaccggaaa
    11281 gcagcagaac attttgcagt ttctttccag ggggcgacgc caccacgtcg atgccgctct
    11341 ccattgagtt ctcaaggtca gtgagccgca tgaacgcctg gcctccaccg aacagcttgc
    11401 tgaacgtgcg cgcgaagttt tcgttgatgt ggttgaacgc ttcctcaaac ttctggcggg
    11461 agatagtgtc gatctccttg atcgtattct gcgtgttctc gatcgactgc agcaggtcca
    11521 ggcgctgggt ttcgaggaac tcgtggcgct gggcagtctc ctggtactcc tcgagtgcca
    11581 tcatgttcac cggccccatg ttctcgaggc gggtgcgcat ctctttgtag agcgattcct
    11641 cggtggtcag gtcctctccg gcaaggcggg tgagcgcttc atcggaaagc agctcttcgc
    11701 gcggcaagct caattcctgg atgcaggttt cggccatgtg ctgagcgtcg gaatgtagac
    11761 gcgcttgttc tgcggccaac tcaccacggc ggtcgcgggc ggtgtcgagt tcatgacgca
    11821 ggttctttag cctctcttcc agttcgccaa gacgcacacg gacggcctgc aattcctcct
    11881 gcagttcgcg gtcgcgatgc tgcgctgttt cgcgttctgc tgtccagtcg atctgctgct
    11941 cggcgagccg ggtgttttcc gtctcgcgtt gttgcttttc ggcagcagag gagtcgatct
    12001 gtgccttcaa tttgtcgagt cgggaggaga cctcggtcat catcgcttca aggcgttcca
    12061 cagttaccgc cgagccgcga cggcgctctt ccagcgtagc caagcgggcc cgcgagtcgc
    12121 tggcgtgctg ggcagacgca tcgcgctgct gacgcagttc atcaagcttg ctctgcgcga
    12181 cctgcatctc ggcttccaat gcgatcttct ggctttcgtg ggtcgccagc tcggtccggt
    12241 gagccgcaat ggctacctcg cactcgccac attcggccgc cagacgttga gtttcacgtc
    12301 cgtaagtgga caggcgttcg cgcacccggg ccatctcgct ttccagctgc tgcagggtgt
    12361 ggcccgacgt catggcctgg cgctcagctt cgcgcttgtc ttcttcgagg cgctgcgcca
    12421 tcgcggtgat ctcgctaatt tcacgcgtga gcacggccac ccgggcctgc tccgcggcca
    12481 tcgcaccttc cacgtcggct gcctggcgca acacgtcgcg gagttcgcgc ttcattgcca
    12541 acgggccttc actgcgctgc ttgccgcccg tcacggtgac attatggaaa cactctcccg
    12601 actgcgatag gaagaaggca tcgggatttt gcaaagccag ttcgcggcct acattgggat
    12661 cgggcacgat atagccgttg cccaacttcg gcaggattac ttcgagcgac ttgccgaatc
    12721 catcgagcac tcggatgcaa tgcttgaggg gaacgacggt atcgcgctgc gcgcccgcat
    12781 aggaaccttc ctcgctgagg aaggagaatt tcgcctgcga atcttccggg tggacgagga
    12841 aagtggcgcg gccgtcaacg tcacttcgca acagacgaag gccctcgtcg gcggcgtccc
    12901 atgacttcac cacaacgtag ttcaactcgt cgcgcaggaa atcgtccact acatgctcgt
    12961 agcggtcatc gacttcaaga aaatctgcca gcacgccgac cggagccatt ccacctgaca
    13021 tcgcgcccga cttaaacaga cgacggactg actcggtgga atatccgtgc tcggtgatga
    13081 cggcttccag cgatcccttt ttgccgatca ggctggcgta ttccgcgcgt aacgaatcca
    13141 ggtgctgctt gccgttggtc tcggcgctgc gtttctgctc gaggttctga cgcgagtcgt
    13201 cgatctgcgc ggtgagcgcg gcaacctgct gcgaagcgga ttcgaattgc aggccaagtt
    13261 gcccgcgctg tccgccgaag ttttcgagtt gctgattcgc aatgctggtt tcttcttcga
    13321 gtcggtgtgc ctcgcgatcg agagcggcca cgcgctcctc agcctgggtc atgcgattgc
    13381 ggactgagga tgccgcagaa actgcctgca ggatggcggc gcggcgcaat tcctgctgcc
    13441 gctcgacttc catcagcgtt gccgcagccg aggatgcttc ttgctgacgc tgctgcaact
    13501 cattctgggc ctgcgcgacg tcggcgttgg ccgattccag aatttcacgg ttggcttcca
    13561 actcctgctg caggcggcgg agctgttctt gcgtgcttcc cacttcggcc tgcgcccccg
    13621 cggaacgggc atctagttcg gcgcaacgct cttcattagt gcgacggcgc gcgatggcac
    13681 gatcaatttc cagcacgacc gcattcagtc gctcgcgatt ttcattacct tcgcgctcga
    13741 tggcatagcc gcgctgagtg cgctcgccgt gctcggcctc catggtcgcg agggcctcgc
    13801 cgcggacgcg catctcctct ccgatttcgg taagctgtgc ctccagtgac gcagactcgg
    13861 catcgagttg ggtgaacttg ctggccagca ccacccgcag tttcgcgcgc atctcgtcgc
    13921 gcaacctggc atagcgctcg gccttgctgg cctggcgctt cagcgagttc atctgccggg
    13981 tcacttcgtc gaagatgtca ttgatacgcg cgaggttcag cttggcgtct tccagacgtg
    14041 cttctgccag acgcttgcgg gtcttgaact tggtgatgcc ggccgcctct tcaatgatgg
    14101 cgcggcggtc ggtcggcttg ctgctgagaa tttgcccgat gcgtccctgc tcgatgatgg
    14161 cgtaggactc ggggccaagt ccggtgccca taaagatgtc ctgaatgtcg cgcaaccggc
    14221 aaagcttgcc attgagcagg tattcgcttt cgcccgagcg gaagaggcga cgcgtcacag
    14281 cgatttcgcc tgccttaaac gtgcggacaa atttgcggcg tcggatcttt aatacaacgt
    14341 gagttcctgt cgtggtctct gccgcggtag cttctggctg agttgaggct tgaccttcga
    14401 ctgaggcgcc ggcttcgtca tcaccctgcc cagggcggac ttcctcggtg tagcgctcgg
    14461 catcgtcggc tttggcagcg cggatcgacg actcgtccca gtcttcttcg ggcatgtcgt
    14521 cctggatgtc gatttcggga tcgcccgcat ccccgtaaac tccgggatcg atcagagtca
    14581 gcgtgacttc ggccatgcca gtgggtttgc gctcgcgcgt gccggcgaag atcacgtcct
    14641 gcatgttcac gccacgcagc gaccgcgccg attgctcacc taaaacccag gaaatggcgt
    14701 cggagatgtt ggacttgcca cagccgttgg gaccaacaat cgcggcaatt ccgtcaccct
    14761 gaaatttcaa ttcagtcctg tcgcagaacg acttaaatcc caggatttga agcttcttta
    14821 gtttgagcaa cttgtactcc ttattcgacg caacgcttca gttccatccg aggtctcaac
    14881 tggcttacct gaaagtccgg cctaagaaaa actgtgtcaa ccttttgtat gcctgactac
    14941 agtccaaaat gccgaaacgc cgcccccact tcatgacacc agagcaactt tactggtgaa
    15001 atggggaggg gtcaagatag aaagcccaat atttggtatg ccgcctgtgg aaaccccact
    15061 atattgcacc tacattgcga caaatgaaca taaaacgtaa gaggtgagga aagactgcga
    15121 ggaggggaaa accgtaagag caatgccagt gtaagcactg gggagggcag tttcaagggg
    15181 aaagcggccg ggtgtctaag tggtaatcag ccgcgggcag cgccgatcca gcatggcacc
    15241 gagcgaacta gggcgccatt agcgaggatt gagattggcg aagcggccaa catggagtaa
    15301 taaaagcgat cgtcgggcaa tctattggag cgggctccat gaacgcgtat aaacgagaac
    15361 tcgtcggtct gggtgagatc gatccagacc gggatggtgg ccttgagttt gtatcgaaac
    15421 agtttgacga cgccgggccc aaatagcgct atggcagttc ctgaagttgg aagagttgga
    15481 cgcgaccatt gccggagtcg gcgacgaaga ggtgattgaa ggaatcgatg cataagccgc
    15541 cggggccagc gaactcgccc accctttgtc cgggcaggcc aaacgaagcg agaggacggc
    15601 cgacctggtt gtaaacacgg atccgtgaac cgctaaacga agtcacgaag acattccccc
    15661 gctgatcggc cgccagccca ttttcccgac tggttagtgg actgcggtca cggggcaggt
    15721 tgaagtctcc gcggaaggca tactgcgagt ccatgatctg gacgcgagtc cccgagctgt
    15781 ccagaacata cacatggtca tggttgacgg cgatatccgt aggatcttcg aattccccca
    15841 ctccggtgcc gtcgtggtac ttgccgagcc gccgcaacac tttgcccata aggtcgagta
    15901 cgaaaaccag gtttcttgga ctgtcgacca ggtagagcct cccggctttt tgatcgatgg
    15961 cgatcccatg aggactctcg tactcgttct cgcccttaaa gtttccgata tagcgcgcaa
    16021 agttgccgta gcgatcaaac acaacgagca tgccgcgctg cgaatccgcg acataaatgt
    16081 tgtcgtcggc gtcgacagcg acgccggcgg gttggcgaag ccgtcggccc ggacccgtca
    16141 tgatgcggaa ggaagttctt ccggaggggt cgagcacatg aaccgcgctg gcgtccggat
    16201 cactaataat gaggcggtgc cgcgagtcgg tggcgatatg acgcggcgcg gacaacacac
    16261 tgggacgccc ataggcgtag gtgataaatc gattgcgaat ttctgccacc cgcgctggca
    16321 cgtgagcgat cgcctcggca tgcgcaggcg gctcaagact ttctacctcc cgttcattgg
    16381 aaagcagcat cgacgctgga gcatcgctga gcggctggcc atgattccga aaatctgccg
    16441 atggaacgcc ttcgcccgga gagcgtccgg tcacgcctgt gagcctggag gtcgtgtgaa
    16501 aaacggcgtc gggactgaag actcccagat atgtcagcac ctcgccatcc cagagattga
    16561 ggcgaggaaa ggcgcgaact ggttgttcgg tatacgggtt ctcagagaga gagacagttg
    16621 ccgcggcaac cggacgtccc gtaagggtct gggcgaaacc gacgcccaga ctcaggacta
    16681 aagcgaacga cactacagaa gcgtactgcg tggcgcgaag cagcttcatc gaagggcccc
    16741 ttggtcttaa atgtccccga ggaagaacca agcaggatca taagcctgtc ttgacgtaga
    16801 gattgagcaa tatcgcacgg ttgatcagaa tattcccaag tcgggaaata acgatgcgcg
    16861 cctgtcgacc gtgggtaacg aggccgaagg ctaggggcat cctcagagtc ggggatcaaa
    16921 ggcacatgcg gtgactcatt ggtccgacaa caatagcaac gcgaaatcgc gtgggccctt
    16981 cgcatggcgt gccaaataaa acaggccgga agtgccgatc cggcctggtc agtattcagc
    17041 tgcgaggtgt gatccgctcc atggcgctac aggtccgcgc caatcgacgc gaagacacgg
    17101 agtccgacta cgtcagcagc ttgcggcgca ggactcccaa cccagctagt attcccgatc
    17161 ccagcagcac gagggtgccc ggttctggtg tcggagtagc gacaaagccg atcacgttgt
    17221 catttgcgtc ggcgtaaaag cccacgagtt gtcccaggtc gttaacgccg ttaatcaccg
    17281 tggaacccgg tacggccaag ggatcgtcga cggtttgcca gacgccgccg aggtaggtga
    17341 acccgtgcat ggttcccgcc gaatcggtgt agtcgcctac gatttcgccg tggttattga
    17401 tgccgaaggc ctgggtaccc attcccccca gaaagtcgag cgtcgtcgca gaggtaccat
    17461 tgaacagata cccatggagt gcaccggagg aatctgcata gaagccgacc acccagccgg
    17521 cgttgttgat gcccacagcc tggttgtttt gggtgcctcc cggcaggaag ccgtcgagat
    17581 aggtgaaggt tgtgcctgca accatgaaag acctctgcaa cgtcattccg gagggatcga
    17641 gcgaggaata accgacagcc gtgagaccgt cattgacgcc cagaagctgg ttaaacgctg
    17701 ttccaggagc atctacattt gtgaaagatc cgttgtaggt gaagccgtgc gtaacaccgc
    17761 ccccatcggt gtagaagccg tcgttccacc ccgtgttatt gataccaatt acctgcgtct
    17821 gggcagcacc cggaaagttc tcggcggtgt agctgcttgg aagcgtcagc gtgaaaccgt
    17881 tgttggcaac cgcagttccg tcgccaaaat atccggcgat ggtgctggag ttgttgatcc
    17941 ccagcagttg ggtgaaagcc gggtcccccg ggctgatgac agactgaaag ctgtatggtg
    18001 ccgcccaaac catctgggcc attaacgcca aggcgaacac tgccgaggca gagcgcaaaa
    18061 caaaacgttt catcctagtt tctcctagct cgaaaggttg ggaccgttga ggtcctctca
    18121 agttctttgg ttgtcttgtc taataatgta cgagcagggg ccgcggccag aatttccgaa
    18181 cagagctatc aagtactcgc aggaagtgag agagtctggg tcttctctca aaacggagtg
    18241 gccgatcgat cagggctcaa ctcggcgcaa gagcggaact aaccgactat cggaaattcc
    18301 ctgtggagcc cgaagcgatg ttcatagtac acgaattgga gtcgtcaaca gagcaaagga
    18361 cggagaaaat ctgcgtgaga atgtgataaa cgtatggcac tttagtgttt ggtccggcgg
    18421 aattgcgaaa agctctacac ccttttgacg acgatgaaat ttgaccatgc taatttgccc
//
LOCUS       DASRKK010000656         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_552079, whole genome shotgun sequence.
ACCESSION   DASRKK010000656 DASRKK010000000
VERSION     DASRKK010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552079"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            667..1803
                     /locus_tag="VFC15_16060"
     CDS             667..1803
                     /locus_tag="VFC15_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11723.1"
                     /translation="MWRSFLRFAAKSGCPDDYSEELAEQFLASQGIPLAGPAASSRAH
                     VLRAVMWMLSAFSHDGCFHRRRCISDRVPLTPTMSAALDKYERFRVERFGTSPHTMRR
                     CRDTLTLFLHFLSARGVDELAGICASDLSAFVRSRGHLEPRTVAIDTYALRSFLRIGF
                     VLGLIPVDMTPHVPKVRMRNHCHIPTVWKSADIDAVLKAVDRESPTGKRDFAILILAC
                     RLGIRVGDIRALKLDNVDWHRRCLSFRQAKTGGALELPLDDEIAEALIDYLRNGRPPM
                     EYREVFVRVNAPFIPFSATDNLYQILEKYRRLAGVELPARSRRGLHSLRHSVASRLLS
                     SGVPLEEVGSILGHQSLDATRVYTSVDIDALRTAALDVDEDSHE"
     gene            1866..2726
                     /locus_tag="VFC15_16065"
     CDS             1866..2726
                     /locus_tag="VFC15_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11724.1"
                     /translation="MCGINHPTPSLPKDVVEGWVLGRPHQCAKTQQYYIGLIRQLAMF
                     LVRTGFAADVPDGSRTPVVQRYLAARVFTRDEVKKLLAAVDLIAPDGRGPMGGTRRHL
                     AFPVLFRVLYGCGLRCGEALRLVAEDVDLDAGVLLIREGKFRQDRLVPVTGSLLKRLR
                     AYSAALGPRPSDAAFFPAPDGGIYSLGTVYHFFRRAIQVAGIPHHGRRHGPRVHDLRH
                     AFAVHRLEAWHREGADLGVKLPVLATYMGHQNLAGTQRYLRLKASLFPDLTQTLEAAY
                     GCLLPGGAAP"
     gene            2723..3742
                     /locus_tag="VFC15_16070"
     CDS             2723..3742
                     /locus_tag="VFC15_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11725.1"
                     /translation="MKPSEFADRLGEFLSVYLPVTRKVSVNTIRSYRDAFALLLRFLR
                     DQKGIPVEKVSLDNLLPSTLIEWLDHLENARRCSPRSRNQRMSAIRSFLRYLAPTDPA
                     RVQQLHRVLAIPSCRCRHDPVPYLEPNLVAALLSQPDLSMPRGRRDAVLLSVLYDTGA
                     RSQEIVDLRVRDLRLDSPAQVTLTGKGRKTRIVPLMPSTVRLLRDYLRSTGLDRPGAG
                     SEPVFAGRGGVALTRSGLRSILAKHADAARASHPGLAKRLGPHTLRHTKAMHMLQAGV
                     ALIHIRDILGHVDIRTTEAYARSDLEMKRKALEKAAASPSAVLKPNLSSWHVNKDLLD
                     WLTSL"
     gene            3756..3956
                     /locus_tag="VFC15_16075"
     CDS             3756..3956
                     /locus_tag="VFC15_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11726.1"
                     /translation="MLPTGIVMPRTYTFGDDLTHNDNQRIKQNMRCNAYFTATWIRQG
                     RLQTMWPHHFGGVFLQKTRGLS"
     gene            4024..>4247
                     /locus_tag="VFC15_16080"
     CDS             4024..>4247
                     /locus_tag="VFC15_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11727.1"
                     /translation="MRQLVLSVPFELRLLLACRADAFGAMTSLFISEVFRWQRERARE
                     EELKKVRYGALVMQHRFGSSLNLNTHLHAV"
BASE COUNT          792 a         1322 c         1253 g          880 t
ORIGIN      
        1 tttgtttggg gttgtgacgg cacgtgaaag gtggggtgcg gctgcgacaa tgtggcgatc
       61 gctagtgcaa agtggcgcca tcgccacccc actgccatca aatgtgccga tgaactcgac
      121 gaatctggct tggcacgagg ctcgctacgc ctcccggaac cgtgactcag acttccggcg
      181 ggacctcgga ttcgaaaggt gccatgcaag cgtcagcagc cctgcgcgcg ggcaatggtt
      241 ccttggctgc ataccagcgc catgagcccg aaaagaccgt cctgtacaag atagtgtcgg
      301 agcatctgga gacattcttg gaggaagtcc gcgaccatta cgacaagcca ttgcccaaat
      361 acgttgagaa ggagttgcgt gactacttag cttgcggatt gttttatgcg tggataggcg
      421 ttatgcgcgg acgcgcttcg agatcggcac ggcgcggcgg atcaacactc caccgcgcat
      481 gcataatccc ggggaatgtt ctacggaggg ttcgggttct cctgcccaga gtggcgaaag
      541 gagacccaac tgactgctac aacccttccc tcgaaaccgc cctgcctcac tgaccttgtt
      601 gcagcagcga ccaggcacct cgagcacctg cgatatgctc ccattacggt cacccagtac
      661 aggggcatgt ggagatcgtt tctgcgcttc gccgcgaagt ccgggtgccc cgacgactac
      721 agcgaggagc tggcagagca gttcctcgcg agtcagggga tcccacttgc gggtccggcc
      781 gccagctcgc gggcccacgt gctccgcgcc gtgatgtgga tgctctcggc gttctcgcac
      841 gacggttgct tccacagaag gcgatgcatt tccgatcggg tgccgctgac gccgacaatg
      901 tccgcagcgc tcgacaagta cgaaagattc cgagtcgagc ggttcgggac gtccccgcac
      961 acgatgagac gttgccggga cactctcacg ttgtttctcc attttctcag cgctcgcggg
     1021 gtggacgagc tggctgggat ttgcgcctct gacctctcgg cattcgttcg ttctcgcggc
     1081 caccttgagc cccgtaccgt tgccatagac acctatgccc ttcggtcatt cctgcgcatc
     1141 gggttcgtct tggggcttat tcctgtggac atgacgccgc atgttcccaa agtgcgtatg
     1201 cgcaaccact gccacatccc gacagtgtgg aaatcggcag acattgatgc tgtgctcaag
     1261 gcggtagacc gtgaatcacc caccgggaaa agggacttcg ctattctgat tctcgcgtgc
     1321 aggcttggca tacgggtcgg cgacatccgg gccttgaagc tcgacaacgt cgactggcac
     1381 cgcaggtgcc tttcctttcg gcaagccaag acggggggcg cgctcgagtt gccgctcgat
     1441 gacgagatcg ccgaggccct aatcgactac ctgcgcaacg gtcggcctcc aatggagtat
     1501 cgcgaggtct ttgttcgcgt caatgcgccg ttcataccct tcagcgcaac ggacaatctg
     1561 taccaaatcc tcgagaagta caggcggctg gccggtgtcg agctgcccgc ccgttcgcgg
     1621 cgcggattgc acagccttcg gcattcagtc gcatcgcgac tgctgtccag cggtgtgccg
     1681 ctcgaggaag tcggcagcat cctgggacac cagtctctcg acgcgacgag ggtctacacc
     1741 agcgtcgata ttgatgccct gcgcaccgcc gcgcttgacg ttgacgagga cagccatgag
     1801 tgatgtcggg ctgagctcgg tgctatccgc ccagattgct gagttcttgg cggagaaaca
     1861 ggtatttgtg cggcatcaat cacccgacgc caagtctccc caaggacgtt gtggaaggct
     1921 gggtacttgg ccgaccgcac cagtgcgcca agacccagca atactacatc gggttgattc
     1981 gacaacttgc catgttcctg gtgcggacgg ggttcgctgc agatgtccct gacgggtcac
     2041 ggacgccggt ggttcagcga tacttggcgg ctcgcgtgtt cacccgcgac gaagtgaaga
     2101 agttgctcgc cgctgtcgac ctgatcgcgc cagacgggcg gggaccgatg ggcggaacac
     2161 gaaggcacct cgccttcccc gtgctgtttc gggtgttgta cggatgcgga cttcgttgcg
     2221 gggaagcgct gcggctggtt gccgaggatg tggacctgga cgcaggcgtg ctgctgattc
     2281 gagagggcaa gttccgacag gaccgcctcg tgcccgtcac cggatccttg ttgaagcgtc
     2341 ttcgcgccta ttccgcggcg ctcggccctc gcccatcgga tgcggcgttc tttccagcgc
     2401 ccgatggtgg catctactcg ctcggcactg tctaccactt cttccgcaga gccattcaag
     2461 tcgcagggat cccgcatcac gggcgccgtc acggaccccg cgtccatgat ctgcgtcatg
     2521 ccttcgctgt ccatcgcttg gaggcgtggc accgcgaggg cgcggacctc ggcgtgaagt
     2581 tgcccgtgct cgcaacctac atgggccacc agaatctggc tggaacgcag cgctatttgc
     2641 gtctgaaagc gtcgctcttc cctgacctga cgcagaccct cgaagcagcc tacggctgtc
     2701 tcttgccggg cggggcggcc ccatgaagcc gagcgaattc gccgacagac tcggggaatt
     2761 tctctcggtg tacttgccgg taacgcgcaa ggtcagcgtc aacacaattc gctcttaccg
     2821 agacgcattc gcgttgctcc tgcggttcct gcgcgaccag aaagggatcc ccgttgagaa
     2881 ggtcagtctc gacaacctcc tcccttcaac gcttatcgaa tggctcgacc atctcgaaaa
     2941 tgcgcgacgg tgctcgcccc gatcccgaaa ccaacgcatg tccgcgatcc gttccttcct
     3001 ccgatacctt gcaccgactg accccgcgag agtccagcaa cttcacaggg tgctggcgat
     3061 cccctcgtgc cggtgtcgac acgatccggt tccctatctc gaaccgaatc tcgtcgcggc
     3121 gcttctttcg cagcccgatc tgtcgatgcc ccgtggccgc cgcgatgcag tcctgttgag
     3181 cgttctctac gatactggtg cacgctcgca agagatcgtc gacttgcgag tacgcgactt
     3241 gcgactcgac tcacccgcgc aagtaacgtt gaccggcaaa ggacgcaaga cgcgtatcgt
     3301 tccgttgatg ccgagcacgg tgcgccttct ccgagactat ctgcgctcca ccggactcga
     3361 ccgtccgggt gcgggcagcg agcccgtgtt cgccgggcgg ggtggggtcg ctctcacgcg
     3421 gtcgggattg cggtccatcc tcgcaaaaca cgctgacgct gcccgggcga gccacccagg
     3481 ccttgctaaa agactcggcc cacacaccct tcggcacacc aaagccatgc acatgctcca
     3541 ggctggcgtc gcattgatcc atatccgaga catcctcggc cacgtggaca ttcgcaccac
     3601 cgaggcctac gcacgatcag accttgagat gaagcgcaaa gccttggaga aagctgcggc
     3661 gtcgcccagc gctgtgttga agcccaatct gagcagttgg cacgtaaaca aggacctgct
     3721 cgactggctc acctcgcttt agggaggtca attatgtgct gcctaccggc atcgtgatgc
     3781 cgcgaaccta caccttcggt gacgatttaa cgcataatga caaccaacgc ataaaacaaa
     3841 atatgcgctg caacgcatat tttactgcaa catggattcg ccaaggccgt ctgcaaacaa
     3901 tgtggccgca ccattttggt ggcgttttcc tgcaaaaaac gcggggcctg tcctagttgc
     3961 tccgccaggc gtatgtgcaa cggtgcggcc catctcgtgg accatgtgct cccggatgtt
     4021 cctgtgcgcc agttggtatt gagcgtgcct ttcgaattgc gcttgctact ggcttgccgt
     4081 gccgatgcct tcggcgccat gaccagcctt ttcatcagcg aggtgtttcg ctggcagcgc
     4141 gaacgggcgc gagaggaaga gttgaagaag gttcgttacg gagcgctggt gatgcagcat
     4201 cgttttgggt cgtctttaaa tcttaacacg cacctccacg cagtttt
//
LOCUS       DASRKK010000657         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1138630, whole genome shotgun sequence.
ACCESSION   DASRKK010000657 DASRKK010000000
VERSION     DASRKK010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138630"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..406)
                     /locus_tag="VFC15_16085"
     CDS             complement(14..406)
                     /locus_tag="VFC15_16085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZM11728.1"
                     /translation="MKISSHFGKPPTGPGAQGMGNRRERKYRRFRLECPIRLKFRAAN
                     RATEVETISRNLSIGGLLVNSAAMIPEHTPVTFIISVLGEQAVRPIYLAGEGKIVRVE
                     HSQVDATFAIAVECKTPIMQLEEHLPPA"
     gene            complement(540..995)
                     /locus_tag="VFC15_16090"
     CDS             complement(540..995)
                     /locus_tag="VFC15_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HZM11729.1"
                     /translation="MRDGIKMLKMLQAVLAVAILIVGIGPQANCYEDQDSQNCCGDSQ
                     ANPSSNVTAAPSGSAASSSKPHSLRLSWNASIPASNSPADAIRGYNVYRRSPGKEYEK
                     INTDLIRGTSCVDYLVKTGQTYYYETRAVSANGAVSKPSSEVRAVIPSR"
     gene            complement(1363..2328)
                     /locus_tag="VFC15_16095"
     CDS             complement(1363..2328)
                     /locus_tag="VFC15_16095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM11730.1"
                     /translation="MWAQHILRCIGDAIISTDISGRINYLNAAAERVTGWSVQEAVGR
                     PLREVMKVIDAARGESDRYLTEAVVDQDAVAVADLAANRILVRRDGTETVIEESVAAI
                     QDQEGRATGAVIVLRALTEEAKAKALKMSHLAQYDGLTDLPNRTLLKDRLSRAFALAT
                     RYSRSLAVLFVDLDHFKQVNDSAGHAIGDRVLQSTAGRLLACVRSSDTVSRYGGDEFV
                     ILLSEIDRSRSLIRVAKKILAAIAVPHDLLTPHVSNITASIGVSIFPQDGRDGETLIQ
                     NADAAMYWVKQHGRNNIQFFDGRLKLKAARQKCPPTNSRTFRRMR"
BASE COUNT          710 a          837 c          788 g          662 t
ORIGIN      
        1 gtgagcaaga agtttatgcc ggcggaagat gctcctccaa ttgcatgatg ggagttttgc
       61 actctaccgc aatcgcaaac gttgcatcaa cttggctgtg ttcgacccgc actatcttac
      121 cttcacccgc caggtaaata ggacgaacag cctgttctcc tagcacgctt atgatgaagg
      181 ttaccggtgt atgctccgga atcattgcag cactgttcac caagagcccg ccgatgctca
      241 ggttccggct gatggtctcg acttcggtgg cgcggttagc agcccgaaac tttagacgga
      301 tcgggcattc caagcggaag cgacggtatt ttcgctcccg ccgattgccc atcccttgtg
      361 ccccaggccc agtcggcggc ttcccaaaat gagaagaaat cttcatgaac gaattctcct
      421 tgttgccgag aagcattggg tgaaatctcc acgacacttg gcagtagcgc cgcttgcgcg
      481 cttgtctaac cgcctatctg cagttaacag tcggcggaga acaggacgcc ggtcgaggtt
      541 caccgagaag gaatcactgc ccttacttcg gacgaaggtt tgctgaccgc tccatttgca
      601 ctaacagctc tggtttcgta atagtaggtc tgtcccgtct tgacgagata gtcaacacag
      661 ctcgtaccgc gaatgagatc tgtgttgatc ttctcgtact ctttacccgg actgcgacga
      721 taaacgttat aacctcggat cgcatcagcg ggagaattgc tcgccggaat acttgcgttc
      781 caggaaagcc tcaggctgtg cggcttggag gaagaggccg cggatcccga cggcgcagca
      841 gtaacgtttg aggatgggtt agcttgcgaa tcgccacaac agttttggct gtcctggtcc
      901 tcgtagcagt tggcctgcgg accaattccg acaatcagaa tagcgacagc caagacggct
      961 tgaagcattt tgagcatctt gatgccgtct ctcacgcacg cctcagtctt ttacggacaa
     1021 ccaccgcatg cgcgcagacg tacttacgat taaaatagtg gttgtggggt gcgaattgcc
     1081 tttaaaccac tctcggtctc tctctgcaga attggctgca ccatcacagg aagttgttgg
     1141 gtgttgcctt cgacaccgca cttcgcccgc taatataggt atcctccggc agagccggag
     1201 gtactacaat tgctggcccc ttgagcctga tcgcaatcgg tgaaagtcaa aagcaaggag
     1261 ccataaactt cgtgtcccga taggtgcctc gtagacaagg agaagcgcgc ttccggaact
     1321 gtcaaacttt tactgcgtcc cctgctgagc cggggagacc tcctaacgca ttcgtcggaa
     1381 agtccgagaa ttggtagggg gacacttttg cctggccgct ttaagtttca gtcgtccatc
     1441 gaagaactga atgttgtttc gtccatgctg cttgacccaa tacattgctg cgtctgcgtt
     1501 ctggatcaac gtttccccat cccgaccgtc ctgtggaaag atgctgacgc cgatactagc
     1561 ggtgatatta gaaacgtgag gcgtgaggag gtcatgtggg accgcgatgg cagcgagaat
     1621 cttttttgca acgcgtatca aacttcttga ccgatcgatc tctgacagca gtatcacaaa
     1681 ttcatcgcct ccatagcggc ttactgtgtc cgaactgcgt acacaagcta gcaagcgccc
     1741 ggcagttgac tgaagcactc ggtcgccaat cgcatgtcct gcggaatcgt tgacttgttt
     1801 gaaatgatcc aagtccacaa aaagcaccgc cagtgacctg ctataacggg tcgccagcgc
     1861 aaacgcgcga ctaagtcgat ctttcagaag cgttcggttg ggcaaatcgg taagaccgtc
     1921 gtattgcgcc aggtgcgaca ttttgagagc cttggccttc gcttcttcgg ttagggcacg
     1981 aagaacgatc accgcacctg tagcgcgacc ttcctggtcc tgaatggcag cgactgattc
     2041 ttcaatcaca gtttccgttc cgtctcgtcg aacgaggatg cggtttgcag ccagatcagc
     2101 caccgccacc gcatcctggt caaccactgc ctcggtgaga taccgatcgg actcgcccct
     2161 agctgcgtcg atgaccttca tcacctctct aagtggccgg cccactgctt cctgaactga
     2221 ccagccagtc accctttcgg ctgcggcgtt gagatagttg attctgccag agatgtcggt
     2281 actgatgatg gcgtccccga tgcacctgag aatatgctgc gcccacaccg cgggatcgga
     2341 ttgccatccc gatgtaattt ggccctcgga gcccgagtta ttgaagctct tagattccaa
     2401 aacctcacca atccctacgt attagtctag tgaaccgccg gctgagccat cagcttgtgc
     2461 tctgcccacc ttacgagcca gagtaggacg aacaagcctg ccagaaatac caggaagaac
     2521 aatatccctc cctggtgatg tagccggcca tgcaggaaac tgcgatctac ataaactgca
     2581 agcgtcgaca gggcgaagat tcgaagcccc ttcttggcaa tggaaagagg aattgccgcc
     2641 agaatgacga ggccttggcc ccaagcggaa cgaagccaaa ggtgggcaag caccatagtg
     2701 gtgaccaaca acatcaggct agagcgaata ctgctgcact gctcagcaac ctcaagcgcc
     2761 aggccgggga tagtcagtcg gagaccatcc tgtgtcaccg gtacgcgagc gatttcgaaa
     2821 agcagattgg cggcgaacgc tgagccctgt tgcaagaagc ttacgtgtgg tttaacgcaa
     2881 actcaggaag agggaccaac cacagcaaga agcacagcgg gaaaacggac atccgggaga
     2941 tacgggtgcc gaaacaacaa atgaacgacc caatccacca tgtgacgaca gccaaca
//
LOCUS       DASRKK010000658         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_64497, whole genome shotgun sequence.
ACCESSION   DASRKK010000658 DASRKK010000000
VERSION     DASRKK010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64497"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFC15_16100"
     CDS             <1..382
                     /locus_tag="VFC15_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM11731.1"
                     /translation="YQIVMDNTGGTGVDIVLEMAGRTESIRTGFKILRLGGRMSLLGI
                     PSKPVELNLAEEVIFKGATVQGINGRRMYQTWYQMTALLKANKLDLHPVITNRIAMKD
                     FASGMELLKTGEASKILVYPNGTK"
     gene            438..1199
                     /locus_tag="VFC15_16105"
     CDS             438..1199
                     /locus_tag="VFC15_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11732.1"
                     /translation="MDRRFRLFPNATWAKFAAIALIAFVASLAALYGSLLGMKGYSAK
                     QASTVLSQLESLKIGDPASSFDHAVSGLRTEAGVHVLTAGAFQFEQLWNLVWKLPQEW
                     ADELMYISSRAGLRWWRLTTSASVQEGRIRRISVGFTVVGRYEMLGAGWSLAPDIPSF
                     YERMPLTEDERRTHMGWFHITSMPSGEGFRIDATGQSTEKELHARRINSKCLLSFRGC
                     DGLCDLLPRAVPVLRDRKSDWGGYTNVPRPACDPR"
     gene            complement(1221..1514)
                     /locus_tag="VFC15_16110"
     CDS             complement(1221..1514)
                     /locus_tag="VFC15_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZM11733.1"
                     /translation="MKNTRRQRKPVSAEAIAHLAEQGRDVSRFFTNRGRMMPPIQRVN
                     VDFTAPMLEELDKRAEDLNISRQAVIKTLIRQALDQHYLATAATTVRRMTKAR"
     gene            complement(1498..1776)
                     /locus_tag="VFC15_16115"
     CDS             complement(1498..1776)
                     /locus_tag="VFC15_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZM11734.1"
                     /translation="MRLHFDERKSQRLRANPRRGIGFEEVQEIFSHPYYLDQRSDVPE
                     QHRAIGWVGGRLYTVIFEVREDAQGEYCHLVTLWKATRQEQQLYEEHS"
     gene            1896..3110
                     /locus_tag="VFC15_16120"
     CDS             1896..3110
                     /locus_tag="VFC15_16120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZM11735.1"
                     /translation="MPTTTRTNPLSYLADQLNELKQKGTFFKLRVLDDEQAAVCTFDG
                     KKVINLASNNYLGLTTHPKLREAALEATKKYGVGSGAVRTIAGTMKIHMELEERIAHF
                     KNVEACVVFQSGFTANAGTVSALLGKEDFIVSDELNHASIIDGARLSRAKIKVFRHKD
                     AAHAEELLHEIKNEPGRKLIITDGVFSMDGDIGALPQLTELAEKYGAIMMVDDAHASG
                     VLGRNGRGTVDHFKLHGRVDVQVGTLSKAIGALGGYVCGTRDLIEFLYHRGRPFLFST
                     SHPPSVAATCIAAFDVLEQEPERIEQLWENTRYWKKELGALGFNIGGVNTPASETPIT
                     PIIVGDGRLTMDFSRELFKEGVMGTGIAFPTVPEGKARIRTIMTATHMREQLDQALDV
                     LKRVGKRMGILA"
     gene            3522..>3811
                     /locus_tag="VFC15_16125"
     CDS             3522..>3811
                     /locus_tag="VFC15_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11736.1"
                     /translation="MALALHVTDEATTGFLDVYNPTVIALRAKLGWWPMPTFEFRGWL
                     FGLIAACVVLLGLSVVVFRGARWIWGFAYFFATVMLLNAAGHTLATIFGTTVG"
BASE COUNT          787 a         1151 c         1093 g          780 t
ORIGIN      
        1 gtaccagatc gtgatggaca acaccggcgg caccggcgtc gacatcgtgc tggagatggc
       61 cgggcgcacc gaatccatcc gaacaggatt caagatcctg cgtctcggtg gccgcatgtc
      121 cctgctcgga attccctcca agccagtcga acttaacctc gcggaagaag tcatcttcaa
      181 gggcgcaacc gtgcaaggca tcaacggccg ccgcatgtac cagacctggt atcagatgac
      241 cgcgctgctc aaggccaaca aactcgacct gcatcccgtc atcaccaatc gcatagccat
      301 gaaggatttc gcgagcggca tggaactgct gaaaaccggc gaagccagca agatcctggt
      361 gtatcccaac gggacgaagt gagagcccgc gtcggagaaa tctggaactt cttgcgaccg
      421 catggggcct aaatgacatg gaccgtcgct tcaggctatt cccgaacgca acgtgggcga
      481 aatttgccgc catcgctctc atcgccttcg tcgcgagcct agccgccctt tacggatccc
      541 tgcttggaat gaagggctat tcggccaagc aagcttcgac ggtgctgagt cagcttgagt
      601 ccctcaagat cggcgatccc gcctcgtcct ttgaccacgc agtatccggc ttgcggaccg
      661 aagcaggtgt acatgtcctg actgccgggg catttcaatt cgaacagcta tggaatttgg
      721 tatggaaact gccccaggaa tgggcagatg agctgatgta catttcgagt cgcgccggat
      781 tgcgatggtg gcggcttaca acttcagcat ccgttcagga ggggagaatt cgtaggattt
      841 ccgttggatt cacggtggtg ggtcgttatg agatgcttgg agcgggttgg agtttggcac
      901 cagacattcc gtcgttctac gaacgaatgc ctttaaccga agatgagcgc cgaacgcaca
      961 tgggatggtt tcacataacc agtatgccaa gcggggaggg attccgcatc gacgcgacag
     1021 gccaaagcac cgaaaaagaa ctgcatgctc ggaggatcaa cagcaagtgt ttgctctcgt
     1081 ttcgcggatg cgatgggctg tgcgacttgc tgcctcgggc agttccggtc cttcgggatc
     1141 gcaagagcga ctggggaggc tacaccaatg tcccacgacc agcctgcgac cccagataac
     1201 acttgtttag gaacctacgt tcaccgtgct ttcgtcatcc gtcgcacggt cgttgccgcc
     1261 gttgccaggt agtgctggtc cagcgcctgg cgaatcagcg tcttgatcac cgcctgccgg
     1321 ctgatgttca aatcctcggc cctcttgtcc aattcttcga gcatgggcgc tgtgaaatcc
     1381 acgttgaccc tttgaatcgg aggcatcatg cggcctcgat tcgtgaaaaa acgcgagaca
     1441 tcccggccct gctcagccag atgcgcgatg gcttccgcgg atactggttt cctttgccta
     1501 cgagtgttct tcataaagtt gttgctcctg cctggttgcc ttccagagag tcaccaggtg
     1561 gcagtactct ccttgcgcgt cttcccgcac ctcaaagatg actgtgtaaa gccggcctcc
     1621 gacccaccca atggcacggt gctgttccgg cacgtccgaa cgctgatcga ggtaatacgg
     1681 atgagaaaag atctcctgca cctcttcgaa gccgattccg cgcctgggat tcgccctcag
     1741 ccgctggctt ttccgctcat caaaatgcaa tcgcactggt ataacagtta tactagatct
     1801 tccgcgtgac gtgcctccga ttccgggctt ctgccctcgg gacataatcc caggcgaaag
     1861 cgcgataaaa tatcccatcg catccggaga ccctcatgcc aaccaccacc cgcaccaacc
     1921 cgctctccta cctcgccgac caactcaacg aactgaagca gaagggcacc ttcttcaagc
     1981 tgcgcgtgct cgacgacgag caggcggcgg tgtgcacctt cgacggcaag aaggtcatca
     2041 acctcgcgtc caacaattac ctcggcctga cgacgcaccc caagctacgc gaagccgccc
     2101 tcgaagcgac gaagaagtat ggcgtcggct ctggcgcggt gcgcaccatc gccggcacca
     2161 tgaagatcca catggagctg gaggagagga tcgcgcactt caagaacgtc gaagcctgcg
     2221 tggtctttca gtcgggcttc acggcgaatg cgggtacggt gtcggcgctg ctcggcaaag
     2281 aagacttcat tgtctccgac gagctgaacc acgccagcat catcgacggc gcgcgcctgt
     2341 cacgcgccaa gatcaaggtc ttccgccaca aggacgccgc ccacgccgaa gagctgctgc
     2401 acgaaattaa gaacgagccg ggccgcaagc tgattatcac cgacggcgtc ttctccatgg
     2461 acggcgacat tggcgcgctg ccacaactta ccgagctcgc cgagaaatat ggcgccatca
     2521 tgatggtgga tgacgcccac gcttccggcg tgctgggccg caacgggcgc ggtaccgtcg
     2581 accacttcaa actgcacggc cgcgtggacg tgcaggtggg cacgctgtcg aaggccatcg
     2641 gcgcgctagg cggttatgtt tgcggcacgc gcgatctcat cgagttcctc tatcatcgcg
     2701 ggcggccgtt cttgttctcc acgtcgcatc cgccgtcggt tgcggccacc tgcatcgccg
     2761 ccttcgacgt gctggagcag gagcccgagc gcatcgagca gctctgggag aacacgcgct
     2821 actggaagaa agagctgggc gccctcggct tcaacatcgg cggagtgaac acgcccgcca
     2881 gcgagacgcc gatcacgccc atcatcgtgg gcgacggccg cctgaccatg gacttctcgc
     2941 gcgagctgtt caaagaaggc gtgatgggca cgggcatcgc gttccccacg gtgcccgagg
     3001 gcaaggcgcg tatccgcacc atcatgaccg ccacgcatat gcgcgaacag cttgaccagg
     3061 cgctcgacgt cctgaagcgc gtcggcaagc gcatgggcat tcttgcgtga tgggtttcgc
     3121 aatatatgaa gctcagcttt gaaggggcac gccttcaggc gtgccgtaaa ggttcactat
     3181 gaattcggct ttagccggtg agggacttcg ccttttagac cattttcaca cttggtgtat
     3241 agggtggagc gccgcttcag cggtgcagtt aggattcggc aatgagacgg ctttagccac
     3301 tgaggtgacg gaaacattac ctcaggcgct aaagcgcgat ccaagaattg acggcagtta
     3361 tgcacgcctg aaggcgtgct ccacccccct atacaccaga tgtgaaactg ctatagccac
     3421 agctgcaacg tacgcgcatc accctatgcc gaggaggtgc acccaagcat gcctccaatc
     3481 cgcagtgtcg ctcgctcggt tgggcatggg ttttactgtg tttggcgctg gcactgcatg
     3541 tgaccgacga ggccaccacc ggctttctgg atgtctacaa tcccaccgtg atcgcactgc
     3601 gggcgaaatt gggctggtgg ccaatgccga ccttcgaatt tcgtggctgg ctcttcggcc
     3661 tgatcgcagc ctgcgtcgtg ctgctagggc tttcggttgt ggtgtttcgc ggcgcccgtt
     3721 ggatttgggg tttcgcgtat ttcttcgcta ctgtgatgct gttaaacgct gccggacata
     3781 cgctagccac aatcttcggc acaacggttg g
//
LOCUS       DASRKK010000659         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_454860, whole genome shotgun sequence.
ACCESSION   DASRKK010000659 DASRKK010000000
VERSION     DASRKK010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454860"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFC15_16130"
     CDS             complement(<1..597)
                     /locus_tag="VFC15_16130"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZM11737.1"
                     /translation="MKIHEYQGKAILSKYGVAVPRGHMVESREAADTAAKALFHDGAT
                     GVVVKAQIHAGGRGKGGGVKIAKSPEEAGVLAAKMLGMNLVTHQTGPEGKKVQRLLIE
                     ETLPIARELYLGIVLDRTNGRLVFMASAAGGMEIEEVAAKDPNAIIKQHLVPGFGLQA
                     FQAREIAFKLGLDAKYIGGAVKFMKALARAAEDTDASLV"
     gene            746..1324
                     /locus_tag="VFC15_16135"
     CDS             746..1324
                     /locus_tag="VFC15_16135"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HZM11738.1"
                     /translation="MLFFSEPSEKQINRFLERASSLEFFYSELGATNSAMLPPLFTVD
                     RNRIQLGSGSLAWHRSIDALRKWEMFNMPWVRLCWPTAPIAERSNVAVLAHRIGLFWL
                     NACRIVYLIDEDGPTKRFGFAYGTLTEHAESGEERFLVEWNRETDAVSYDLLAFSRPN
                     QFLSRVGYPVARRLQKQFAADSKAAMVRAVAV"
     gene            1515..2579
                     /gene="trpD"
                     /locus_tag="VFC15_16140"
     CDS             1515..2579
                     /gene="trpD"
                     /locus_tag="VFC15_16140"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZM11739.1"
                     /translation="MLLDALHKIANHRTSLTREEAREVMSQILRGEATDAQIAALLVA
                     LHMKGETVEEIVGFAQAIRDEAMPLQTEDGEALDLSGTGRDALVDTCGTGGDASGTFN
                     ISTATALAIAGAGVRVAKHGNRSISSKCGSADVMEALGVNINLPTARIAACLRETGIA
                     FLFAPALHSAMKYVQPARRELRLRTVFNLLGPLTNPARASAQVVGVYSSDLVEKLAEA
                     LSELGLRRALVVHGRDGLDEITISGPSKVAEVRNGQVHVYEVTPEDFGLQRAPLEDIA
                     GGDAQQNAAIIRAILDGERSCRRDVVLLNAAAALVAAGHADRIGEAVPLAAYAIDSGH
                     ARQRLQLLVEFTQQGLGARG"
BASE COUNT          550 a          857 c          864 g          591 t
ORIGIN      
        1 gaccagcgag gcgtcggtat cttccgccgc gcgcgccagc gccttcatga acttcaccgc
       61 gccgccaata tacttggcgt cgagacccag tttgaaggcg atctcgcgcg cctgaaacgc
      121 ttgcagcccg aagccgggga cgagatgctg cttgatgatg gcgttgggat ctttggccgc
      181 gacctcttcg atctccatgc cgcccgcggc ggaagccatg aataccaggc gtccgttggt
      241 gcggtccagc acgataccga gatacagctc gcgcgcgatg ggcaaagtct cttcaatcag
      301 caggcgctga actttcttgc cctcgggacc ggtctggtga gtgaccaggt tcatcccgag
      361 catcttggcc gccagcacgc cggcttcttc cggcgacttg gcgatcttga cgccgccgcc
      421 tttgccgcga ccgccagcat ggatttgtgc cttgacaaca acccctgtcg cgccgtcatg
      481 aaacaaggcc ttggccgcag tgtcagctgc ttcgcggctt tcaaccatgt gcccacgcgg
      541 caccgccact ccgtacttcg agaggatggc ttttccctgg tactcgtgta ttttcatgtt
      601 tgtttgattg cggcgagcga ctgactcgct gctgtggccg gatgggccag acgtgtctgc
      661 tccttatgag gatcgcgtaa caataggaaa ctcatgattg taacggacgg gaagagtcgc
      721 gagcaattcg cgattcggag gtacagtgct ctttttctca gaaccttctg aaaaacagat
      781 caatcgcttt ctggagcgcg cttcatcgtt ggaatttttc tactccgaac tgggtgcaac
      841 aaattccgca atgctgccgc ctctgttcac ggtagatcga aatcgcatac agcttggctc
      901 cggatctctt gcgtggcacc ggtcgatcga tgcgctcaga aaatgggaga tgttcaatat
      961 gccgtgggtc cgcctctgct ggccaacagc tccgattgca gaaaggtcga acgttgccgt
     1021 cctcgcgcat cgtatcggcc tgttctggct caatgcgtgt cgcattgtct atctgatcga
     1081 cgaggacgga ccaacaaagc gtttcggatt cgcctatggg acgctcacag aacatgcgga
     1141 atccggcgag gaacgtttcc ttgttgaatg gaaccgtgag accgacgctg tctcttatga
     1201 tctattggcc ttctcgcgtc ctaaccaatt cttgtcgcga gtcggatacc cagtcgcgcg
     1261 tcggctgcag aaacaattcg ccgcggactc gaaagcagcc atggtccgag ccgttgctgt
     1321 gtgaacagag gaccaatcct agtctgccgg ataggggtcc ttcgactcct cggtcgccac
     1381 ggcgacctcg tcgctcagga tgacacaaca agatgacaca acaaaaggta tgatgttcgc
     1441 gggctgaatg ctgagtgcta acaactgata gctgatggct ggcaactgac aactggcaac
     1501 tgacaactga ctccatgctc ctcgacgcgc tgcacaaaat cgccaaccac cgcacttccc
     1561 tcacccgcga ggaagcgcgc gaagttatgt cgcagatctt gcggggtgag gccaccgacg
     1621 cgcagattgc ggcgctgctg gtcgcgctgc acatgaaggg cgagacggtg gaggagatcg
     1681 ttggattcgc acaggccatt cgcgacgagg ccatgccgtt gcagaccgag gacggcgagg
     1741 cgctcgacct cagcggcact ggccgtgacg cgctggtgga cacctgcggc accggcggag
     1801 acgccagcgg cacgtttaac atttccaccg cgacggcgct ggctatcgcc ggggctggag
     1861 tgcgagttgc gaaacatggc aaccgtagca tcagctccaa atgcggctcg gccgacgtga
     1921 tggaggcgct cggagtcaac atcaacctgc caacggcgcg cattgcggcc tgcttgcgcg
     1981 agaccggcat cgctttccta ttcgcgccag ctctgcattc ggcgatgaag tacgtccaac
     2041 cggcgcgccg cgagttgcgg ctgcgcaccg tctttaacct gctggggccg ctgaccaacc
     2101 cagcgcgcgc ttcggcgcag gtggtcggcg tctattcctc cgacctggtg gaaaagctgg
     2161 ccgaggcgct gagcgaactt gggttgcgcc gtgctctggt ggtccatgga cgcgatgggc
     2221 tagacgagat caccatctcc ggccccagca aagttgccga agttcgcaac ggtcaggtcc
     2281 acgtgtacga agtcacgccg gaagattttg gcttgcaacg cgcaccactc gaagatatcg
     2341 cgggaggcga cgcccagcaa aacgcggcca ttatccgcgc gattctcgat ggcgagcgct
     2401 cctgccgccg cgatgtggtt ttgctcaacg ccgccgcggc gctggtcgcc gccggacacg
     2461 ccgatcgcat cggcgaggcc gtgccgctgg ctgcctacgc gatcgacagc ggccatgcgc
     2521 gccagcggct gcaattgctg gtggagttta cacagcaggg gctaggggct aggggctagt
     2581 ggctagtggt cagcgcgacg gttccgattg ggctgtcgtc ctgccgcggt tccaggattg
     2641 gtgtatccgc tcgttcgggt agtgcgggcc ttcaggcccg cgtccaaggg cgagaagaaa
     2701 aatcgcttca gccgctgagg tagaggtctg gttacctcag cggctaaagc cgaattcaga
     2761 ggcaactctc aaatgcaccg ctgaagcggt gctccacccg gatgcaccaa ctcataatct
     2821 gctctaatat ttattatttc gcccctgcgg ggctggattt tg
//
LOCUS       DASRKK010000660         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_258561, whole genome shotgun sequence.
ACCESSION   DASRKK010000660 DASRKK010000000
VERSION     DASRKK010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258561"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..489
                     /locus_tag="VFC15_16145"
                     /pseudo
     CDS             1..489
                     /locus_tag="VFC15_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            632..1747
                     /locus_tag="VFC15_16150"
     CDS             632..1747
                     /locus_tag="VFC15_16150"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HZM11740.1"
                     /translation="MISYSRLIDALGAIPDPRRARGRRFPLPYLLLFSVLAVLSGAKS
                     FTHIVIFMRERREVLNQVFGSTFKTAPSINTVRLLLHAIGGDPLEATVRGYAEHLVTD
                     AAKEALPLIALDGKTLRGSFDHINDRKAVHTLSALAVAGKIVLGHFEVDGKTNEIPCV
                     PALIRQPGLAGVIYTGDAMNCQKETFLAAAETNSSVLAQVKGNQPALLHALEDLTGTR
                     TPTDRCEVKDKIAHGRQERRRVETFNVAGCLGPDWDELVVTAARVTRLTWHKDTKDGM
                     WTASNDSAIYLCQKSLSAADFGAAIRGHWEVGAHHYVRDVTLGEDASRIRINPLNFAR
                     LRTIALNILRANGVKNVAQALFRNALNLENILSYKLS"
     gene            complement(1785..2126)
                     /locus_tag="VFC15_16155"
     CDS             complement(1785..2126)
                     /locus_tag="VFC15_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11741.1"
                     /translation="MDDPYTLQILDPWTLMYGEYEEEFIEVLRRRIGPKHPLYNREVY
                     AIAIRRDPEATLFHAFNDGFYAIVYFSGVPRGRPGMPKTEIFPDDRAVGEKIAADHAE
                     RLEQYKREREN"
BASE COUNT          540 a          744 c          692 g          518 t
ORIGIN      
        1 ctcgacgact ccgcacgaca agcgctctcc cagctgctgg cacgcgacga cacattgtcg
       61 gaatgggcgg ctctcaagca ggacgccaaa aacttcaaat ggcgtcagat ggcgaacgaa
      121 cgagagaagc gtgccaggct cgaaccgttg tatctgatcg ccaggacgtt gctgccgaaa
      181 cttgcgatct cgcaacgcaa tctgcactat tacgcgagtc tggcaaattt ctatacggtg
      241 tatgacctgc gccggctcaa ggcggagcaa acacatctat acttgctgtg ttacgcttgg
      301 cagcgctacc ggcaactcac cgacaatctc gtcgatgccc tgagctatca catgaagcag
      361 ctcgaggacg agagcaaggc gcggacagac aagcgcttcg ttgccgagca ggcgcaacgc
      421 gagcaggaaa cgccgcgggt cgggcggctg ctgttgctct atgtcgatga cgcggtcgcc
      481 gacatcacgc acggcgattc agttctcagc gaggtgagca tggatacgcg cgtgctgtat
      541 cggacacgtg acgaccaatc ttgaacagtc tagcaaaata tggggaaaat atgggtccta
      601 ttgccacatc ttattaacct gccaggatgc catgatctct tattcccgcc tgattgatgc
      661 tttgggtgca attcccgatc ctcgccgtgc tcggggacgg cgcttcccgc tgccctatct
      721 tcttctgttc tccgtcctgg cggttttgtc gggtgcgaag agttttacgc atattgtcat
      781 ttttatgcgg gaacgtcggg aggttctcaa ccaggtgttc ggcagcacgt tcaagactgc
      841 gccgtcgatc aacacggtgc gcctgctcct gcatgcgatc ggtggcgacc cccttgaggc
      901 gactgtacgc ggatatgcgg agcatctggt gaccgacgcc gccaaggagg ctctgccgct
      961 catcgcgctg gatggcaaga ccctcagggg aagcttcgac catatcaacg atcgcaaggc
     1021 ggtgcatacg ttgagcgcgc ttgcggtcgc cgggaagatc gtcctcggtc attttgaggt
     1081 cgacggcaag accaacgaaa ttccttgcgt gccagcactg atccgccaac ccggcctcgc
     1141 cggcgtgatc tatactggcg acgcgatgaa ttgccagaag gagaccttcc tcgcagccgc
     1201 ggagaccaac agctctgtcc tcgctcaggt caaaggcaac cagccggccc tgcttcatgc
     1261 cctcgaagat ctcacgggca cccgcacacc aaccgatcgg tgcgaggtca aggacaagat
     1321 tgctcacggc cgtcaggagc ggcgccgggt cgagaccttc aatgtcgccg gttgcctcgg
     1381 tcccgactgg gatgaactgg tcgtcaccgc ggcccgcgtc acccgcctga cctggcacaa
     1441 ggataccaag gatgggatgt ggacggcgag caacgacagc gcgatctacc tctgtcagaa
     1501 aagcttaagc gccgccgatt tcggcgccgc catcaggggg cattgggaag tcggggcgca
     1561 tcattacgtc cgcgacgtaa ccctcggtga agacgccagc cggatccgca tcaacccgtt
     1621 gaactttgcc cgcctgcgca ccatcgccct caatatcctg cgtgccaatg gcgtcaaaaa
     1681 cgtcgctcag gcgctcttcc gcaacgccct gaacctggaa aatatcctgt cctacaagct
     1741 gtcgtgagca gcatcgctgg cagagggcgg attacgcctg ctcgtcagtt ctctcgctcg
     1801 cgcttgtatt gctccagccg ttcggcgtgg tcggccgcga tcttttcgcc gacagctcga
     1861 tcgtcgggga atatctcggt cttcggcatc cccggccggc cccttggcac acctgaaaag
     1921 taaacgatcg cataaaatcc atcattaaat gcatggaata aagtggcctc ggggtcgcgg
     1981 cggatcgcta tcgcatagac ctctcgatta taaagcgggt gcttgggccc aatcctccgg
     2041 cgaagcactt cgataaactc ttcttcgtac tctccgtaca tcagagtcca tggatccaga
     2101 atctggagtg tatatggatc gtccatagat atattctcta caatgtcccc aataaattgg
     2161 cacgtcagga gtgccactct cgccagctcc ggcacccgta tatctcaatc cgtaaccgca
     2221 atatatgcac tagcacgtgt cagtctgtca aattcctgcc gcgttatgtg cgtcagttcg
     2281 agaactgaat cgccgtggga tgaacccatc gatcgtcctg caatgatcaa ttttgacccc
     2341 aaagggaaac gggacgcatc tgtataataa cgttatagaa tcaagtagtt ggttgatgtc
     2401 gagaccgatc cgtccgcaca aggatatcga aactgtcgcc aggcagaacc ggatcgcgcg
     2461 gcccggaccc gattggcgag gatccagtgc gggc
//
LOCUS       DASRKK010000661         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_749441, whole genome shotgun sequence.
ACCESSION   DASRKK010000661 DASRKK010000000
VERSION     DASRKK010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749441"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFC15_16160"
     CDS             <1..573
                     /locus_tag="VFC15_16160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZM11742.1"
                     /translation="IAERMHAKSHYYEIAREFGRLVGSANHGARKHHTVVVTGGGPGM
                     MEAANRGAFDVGAKSVGLNISLPHEQYPNPYVTPELCFSFHYFALRKLHFLLRAKALV
                     AFPGGYGTFDELFEVLTLVQTRKIKPIPVVLVGESYWRRAVDMDFLVDEGVIDPEDRD
                     LFWFAETAQEIWDGILHWHDASGEPLHTAT"
     gene            606..2036
                     /locus_tag="VFC15_16165"
     CDS             606..2036
                     /locus_tag="VFC15_16165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM11743.1"
                     /translation="MQFPLELANTQESHLKLSFHGADRSVTGSCHLVECAGKRILIDC
                     GLLQGSRELDEENAEPFGFDAASIDYVLLTHAHLDHCGRLPLLAKRGFRGEIITTSAS
                     RELARLVMLDAAHLHEEDARYRARSGGHDGASHATNEPLYSTLDALNCLDYFGRTAIY
                     SQVVEVAAGVHATFIDAGHILGSASIFLQIEEQSHSTSVLFSGDLGNAGRLLLRSPAQ
                     PPHAENVVMETTYGDRLHKQLGPSIDEFYEAINETFKRGGNVIIPTFALERAQELLYF
                     LSQGVTQGRLTKSTQVYLDSPMAISATEIFRRHPECFEPATAKLFQEGHDPFALPGLH
                     FTRETAESVAINNVHGGAIIMAGAGMCTGGRVRHHLQHNLGREDSSIVFVGYAASGTL
                     ARRIIDGEKQVNIFGENIPVRARIYTINGFSAHADQAELLAWQKQTSTKRTFLVHGEE
                     VTMKEFATHLVDTRVEMPGPNQVFEL"
     gene            complement(2270..3268)
                     /locus_tag="VFC15_16170"
     CDS             complement(2270..3268)
                     /locus_tag="VFC15_16170"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZM11744.1"
                     /translation="MGEFRNSAGRGRTHCNASRSLQRGPTGRGRASRGARGPNLRHHC
                     LVDETVLRQLEAATAFAPLHNPLALSVIRFVQEHFPQLPQVVCFDTAFHAGMPDVART
                     LPIPLEMRSDGIRRYGFHGLSCESIMRQLGGNRPDRLIIAHLGNGASLTAVKGGQSID
                     TSMGLTPTGGVIMGTRCGDLDPGVLVFLVREKNFDAARLEALVDHHSGLLGISGIDSD
                     MRRLHEVSPSNADARLAIEMFCYSIRKQVAAMIAALDGVDLLVFTGGIGENDAKVRAE
                     ICGGLSWLGISLDTTRNHSLEDPISNPTSRCSVRVLPSEEDAQVATHTWELASGIS"
     gene            complement(3462..>3948)
                     /locus_tag="VFC15_16175"
     CDS             complement(3462..>3948)
                     /locus_tag="VFC15_16175"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11745.1"
                     /translation="DVPTLETLAAVTLLRDYIPDIRVRVVNVVDLMALEPQTEHPHGL
                     EDRFFDELFTTNKPVIFAFHGYPAIIHKLTYRRHNHDNIHVRGYKEEGTTTTPFDMVV
                     LNNLDRYQLALDAIQRIPRLRDQMEKATARYWSTMERHKLYVSEHGEDMPEVQNWRWP
                     S"
BASE COUNT          854 a         1122 c         1121 g          851 t
ORIGIN      
        1 attgctgagc gtatgcatgc caagagtcat tattacgaga tagcccgcga attcgggcgt
       61 ctcgtgggca gtgcgaacca tggcgctcgc aaacaccata cggtcgtcgt aaccggcgga
      121 gggcctggaa tgatggaggc cgccaaccgt ggggcattcg atgtcggggc caagtccgtc
      181 ggactgaaca tcagccttcc tcatgagcaa tatccgaacc cctacgtcac gcccgaactc
      241 tgtttcagct ttcattattt cgccttgcgc aagctgcatt tcctcctgcg ggccaaggcg
      301 ctggtcgcgt tcccgggtgg ctatggcacg ttcgacgaac tgtttgaggt tctaactctg
      361 gtacagacgc gaaaaatcaa gccaatccct gtcgtgttgg taggtgagag ctattggcgt
      421 cgtgcggtcg acatggattt cctcgtcgat gaaggtgtca tcgatccgga ggatcgggat
      481 ctgttctggt ttgccgagac tgcgcaggaa atctgggacg gaattttgca ctggcatgat
      541 gcgagcggcg agcctttgca caccgcaacg tgagagacct aaattgcaat actgctaaaa
      601 cgcccgtgca gtttcctcta gaattagcga atacacagga gagtcacttg aaattatcct
      661 ttcatggtgc cgatcgcagt gtcaccggct cctgtcattt ggtcgaatgc gctgggaagc
      721 ggatcttgat cgactgcggg ctacttcaag gtagccgcga gttagatgag gaaaatgccg
      781 aacctttcgg ctttgatgcc gccagcatcg actacgtgtt gctcacccat gcgcacctgg
      841 atcattgtgg gcgtctgccg ctgctggcca aaagaggatt tcggggcgag atcattacaa
      901 cttcagcctc gcgtgagttg gcgcgactgg tcatgctgga tgcggcccac ctgcatgagg
      961 aagacgcgcg ctaccgcgca cgcagcggcg ggcacgacgg tgcaagccac gctacaaacg
     1021 aaccgctcta ttcgaccctc gacgcactga actgcctcga ttatttcgga cggaccgcga
     1081 tctactcaca ggtcgttgag gtcgccgccg gcgtgcacgc caccttcatc gacgctggcc
     1141 acatcctggg ctcggccagc attttcttgc agattgagga acagagccat tcgaccagtg
     1201 tgctgttttc gggtgaccta ggcaacgctg ggcgccttct gctgcgcagc cccgcgcaac
     1261 cgccacacgc cgaaaacgtc gtgatggaaa ccacatacgg ggaccggttg cacaagcaat
     1321 tgggtccatc catcgatgag ttttatgagg ctataaacga aactttcaaa cgcggaggaa
     1381 acgtcatcat tccgaccttc gctctggagc gcgcacagga gctactctat ttcttgagtc
     1441 agggcgtcac tcagggccgg ctcacaaaat cgacgcaagt gtacctggat tcaccgatgg
     1501 ctatttcagc caccgagatc ttccgacgcc atccggagtg ctttgagcca gcgaccgcga
     1561 aattgtttca agagggacac gacccatttg ctttaccggg attgcatttc acgcgggaga
     1621 ccgccgaatc cgttgcaata aacaacgtcc atggcggcgc catcatcatg gctggcgcgg
     1681 gcatgtgcac cggcggccgc gtgcgccacc acctccagca caatctcggt cgcgaagatt
     1741 cgagcatcgt gttcgtcggc tacgcagcca gtgggactct tgcgcgccgt attatcgacg
     1801 gcgagaagca ggtgaacatt ttcggtgaga atattccggt tcgggctcgc atctacacca
     1861 ttaatggttt ctccgcccat gccgaccagg cggagctgct agcttggcaa aagcagacca
     1921 gcacgaaacg tacattcctt gtccacggcg aagaagtcac catgaaggag tttgcgacac
     1981 atcttgttga cactcgcgtc gaaatgccgg ggccaaatca agtttttgag ctttgaactc
     2041 gcgccttgac gatgctcgta ccgagccgaa tttttgcttg agcaaaaaag ccatccgacc
     2101 tgccaatccc accgccccaa aatatttcag ttagttccag caacacggcg atcctgaaga
     2161 tagcgaggat ttgcatagcc atcgattccg gaaattatgt cgtcttaaac tggaggctta
     2221 taaatcttat ggctcacacc ccactagtgc gttgataacc cgcgtcgaat cacgaaatgc
     2281 ccgaggcgag ttcccaggta tgagtggcga cctgcgcgtc ttcttccgag gggagcacgc
     2341 ggaccgaaca acgcgacgtt ggattgctga tggggtcttc caaagaatgg tttcgtgttg
     2401 tgtcgaggct gataccaagc cacgagagac cgccacagat ctcggccctc acctttgcat
     2461 cgttttctcc gatcccgccg gtaaagacca gcagatccac cccatcgagt gccgcgatca
     2521 tggccgccac ctgtttgcgg attgagtaac agaacatttc gattgccagc cgtgcgtctg
     2581 cgttggatgg agaaacttca tgcagccgac gcatgtcgct atcgattccg gaaattccga
     2641 ggagtccgga gtggtgatcg accaacgctt cgagacgcgc ggcgtcgaag ttcttttcac
     2701 gcaccaaaaa gaccagcaca cccggatcga gatcaccgca gcgcgtcccc atgatcaccc
     2761 caccggtcgg ggtgagaccc atgctggtgt caatggattg accgccttta acggcggtga
     2821 gactggcgcc attgccgaga tgcgcaatta ttagccgatc cggccgattg cccccaagtt
     2881 ggcgcatgat cgactcgcaa gaaaggccgt ggaatccata gcggcgtatg ccatccgatc
     2941 gcatttctag ggggatcggc agggtgcgcg cgacatctgg catcccagcg tgaaacgccg
     3001 tgtcgaagca gacaacttgt ggcagctgcg ggaagtgctc ctgcacgaag cgaatgactg
     3061 acagcgccag aggattgtgc agtggcgcga aggcggttgc cgcttcgagc tgccgcagca
     3121 ccgtttcgtc gaccaggcaa tggtggcgaa gattcggccc ccgtgcacca cgcgatgccc
     3181 gaccgcgacc ggttgggcct ctttgcagac tgcgagaagc gttgcaatgc gtgcgaccgc
     3241 gtcctgctga gttccgaaat tcgccgattc ggaaaccaac ggcttatcgt tcgcatcttt
     3301 cgcccagaac ttgctctggc ggtcgccaat cgactcggct tcgcccgaga gaagcacctt
     3361 tgggatagaa gactcgaccc ggtaaagtcc gaacttgagc gacgatgacc cggagttcag
     3421 ggcaagtact gatgagcgtg gtccagcgct tgtcatacgg cttatgaggg ccagcgccag
     3481 ttttgcacct cgggcatatc ctcaccgtgc tcgctgacgt aaagcttgtg acgctccatc
     3541 gtgctccagt agcgcgccgt tgccttctcc atttgatcgc gcagccgtgg aattcgctgg
     3601 atggcatcca gcgcgagttg atagcggtcg agattgttca gcacaaccat gtcgaaggga
     3661 gtcgtggtgg tgccctcctc cttgtagccg cgcacatgaa tattgtcatg attgtgccgt
     3721 cggtaggtca gcttgtgaat gatcgcggga tagccatgga aggcgaagat caccggcttg
     3781 tttgtcgtga atagttcgtc aaaaaatcga tcttccagcc catgcgggtg ctccgtctgc
     3841 ggctccaaag ccatcaggtc gaccacgttc acaacgcgga cccgaatgtc cggaatgtag
     3901 tctcgcaaga gggtcacggc tgcgagagtt tcgagcgtag gtacatca
//
LOCUS       DASRKK010000662         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1042344, whole genome shotgun sequence.
ACCESSION   DASRKK010000662 DASRKK010000000
VERSION     DASRKK010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042344"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..725)
                     /locus_tag="VFC15_16180"
     CDS             complement(261..725)
                     /locus_tag="VFC15_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11746.1"
                     /translation="MSLALKRVLAPAPGFPRPPAIVETESRLNVAVIFTSVQSTLAAL
                     KKAGALANRLSGRITLVVPQVVPYPLPLTSPPVLLDWNEKRFRVIAGESPVETTVQIY
                     LCRDRLETLTAVLSPHSLVVVGGPKRWWPTAEKRLARTLRRAGHEVILTETE"
     gene            complement(1043..1744)
                     /locus_tag="VFC15_16185"
     CDS             complement(1043..1744)
                     /locus_tag="VFC15_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM11747.1"
                     /translation="MSAGRILVVDDEPQLRRIMRTTLIGAGYEVDDAKTGEEALEKVR
                     EFRPDLVLLDVNMPGMGGVAACRVMREDTNIAIIMLTVRNTEKDKVEALDAGADDFVV
                     KPFSTPELLARIRASLRRVPAAPQSSQERIRAGSLVIDFAARTVTNGDASFHLTPKEL
                     DLLRYLTQHANQAVPHRELLQAIWGPDYGDEVEYLRVLIASLRKKIEPNPEQPEYIIT
                     EPWVGYRFRARPEPE"
     gene            complement(1741..>2491)
                     /locus_tag="VFC15_16190"
     CDS             complement(1741..>2491)
                     /locus_tag="VFC15_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM11748.1"
                     /translation="GLERARAQELAQQIEAAQQSEQLRTTLIDAMSHEFKTPLTSIMA
                     ATTSLLSVPDQPLDTRMELLRIADEEAVHLRDLINDAVEMARLDTANIEVNLERSNLG
                     ELVGDVIASMHKEIDGRPIEVACDGKQPAIAVDQRLVKLAIKQLLDNALKYSAPGTPV
                     TVQIRDGDGMMTVDVTNQGKGIPPAEQARVFERLYRSPSVKKQMPGSGLGLSIAHGIA
                     RAHNGDLTVTSRPGETTFRLILPTEHKGEQG"
BASE COUNT          502 a          722 c          723 g          544 t
ORIGIN      
        1 ttgacgcagg cgagcaccac ggcatagaaa accgcaatct gtaatacgcc attggctgtc
       61 atctgttatc ttctctcctt agaaacgctc cggcagaagc agagcgtaga ccaggtaaac
      121 gaacagggcc accgcggtca aaccggcaat gatgtaatcc atcgctttcc tcctagagct
      181 tgtcgcaggc tttcgtaaaa gcccagcaga caagcaagaa cacacaagct attccgatgt
      241 aaatcagatc gagcatgggt ttactccgtt tccgtgagaa tcacttcgtg accggcgcga
      301 cgcagcgtcc gcgccaaacg tttttcggct gtcggccacc atcgtttggg accaccgacg
      361 acgacgagcg agtggggact cagcacggcg gtcagcgtct ccagtcgatc gcgacacagg
      421 tatatctgga cggtcgtttc tacgggactc tcgcccgcga ttacgcgaaa gcgcttctcg
      481 ttccagtcca gcaggacggg cggactggtg agcgggaggg gatacggaac gacctgcggg
      541 accactaggg tgatgcgacc gctcaggcga ttcgccaagg cgccagcttt cttcagtgct
      601 gccaatgttg actggaccga cgtaaagata acggcgacgt tgagccgcga ctccgtctcc
      661 acgatcgctg gcggtctggg aaatcccggc gccggtgcca ggactctttt aagggctagt
      721 gacatgtcgt tcttcgctcc cacaattgag cgtacgcgga aaggcctaaa gaagtcatag
      781 aaaagccatg aagatttcat aaagaccagc aagtgcccga ccggtcttgg tggtcgccgg
      841 cgcgggccca tctggggggt gagaaccgac tcagggttgc acggcaaccg ttgcgcagta
      901 tacgtgggct tcttgtttcc ttttcgggtc aatgcgatgg gctgtacgga gacgacagcg
      961 ttggcagtga ccgtcggttg ccctttttgg cctgcaacac ttcctgacac gccagagttg
     1021 ccgactcgac gggccggtca catcattccg gttcaggccg ggcgcgaaag cggtagccaa
     1081 cccatggttc ggtgatgatg tactcgggct gctccggatt tggctcgatc ttcttgcgga
     1141 ggctggcgat caagacgcga aggtactcga cctcgtcacc gtagtcaggt ccccatattg
     1201 cctgcaggag ttcccgatgg ggcactgcct gattcgcgtg ttgcgtcaga tatcgaagaa
     1261 ggtcgagttc cttcggcgtg agatggaagc tcgcgtcacc gttcgtcacc gtccgcgcgg
     1321 caaagtcgat cacaaggctg ccagcgcgaa tcctttcctg cgatgactgt ggcgcagcgg
     1381 gcactcgccg gagcgaagca cgtatgcggg ccagaagctc aggagtgctg aacggtttca
     1441 ccacaaaatc gtcggcgccc gcgtccagcg cctcgacctt atctttctcg gtgttccgga
     1501 ccgtgagcat gatgatggcg atgttcgtgt cttctcgcat cactctgcag gcggccacgc
     1561 cgcccatccc aggcatattg acgtcgagca ggaccaggtc aggacggaac tcacggacct
     1621 tctcaagagc ctcctcaccg gtcttggcgt cgtccacttc gtatccggcg ccaatcaatg
     1681 tggttcgcat gatgcgccga agttgaggct cgtcatcaac gaccagaatg cgacctgcgc
     1741 tcaaccttgc tctcccttgt gttccgttgg caagatcaac cgaaaggtag tctcgccggg
     1801 acggcttgtg acagtcagat ctccattatg agcacgggcg atgccatggg cgatactgag
     1861 gccgaggcca gaacccggca tttgtttctt gacggaagga cttctgtaga gtctttcaaa
     1921 gacgcgggcc tgctcggccg gcggaatccc cttaccttga ttggtgacgt ccaccgtcat
     1981 catgccatcg ccatcccgga tttggacagt caccggtgtc cctggagccg aatactttag
     2041 agcattgtcg agaagctgct tgatcgcgag cttcaccaat cgctgatcga cggcgattgc
     2101 aggctgtttt ccatcgcagg ccacctcaat cggtcgaccg tcgatttctt tgtgcattga
     2161 ggcgatcacg tctccaacaa gctcgccgag gttggagcgt tccagattaa cttcaatatt
     2221 cgcggtgtcg agccgcgcca tttccaccgc atcgttgatg agatcacgca aatgcaccgc
     2281 ttcctcatcg gcgatccgga gcagttccat cctggtatcg agaggctgat caggcacgga
     2341 caggagtgaa gttgtcgcag ccatgatcga cgtcagcggt gtcttgaact cgtgcgacat
     2401 ggcatcaatg agcgtcgttc gcaactgctc gctttgctgt gcggcttcga tctgttgggc
     2461 gagttcctgt gcccttgcgc gctccagtcc g
//
LOCUS       DASRKK010000663         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_944265, whole genome shotgun sequence.
ACCESSION   DASRKK010000663 DASRKK010000000
VERSION     DASRKK010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944265"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1515
                     /locus_tag="VFC15_16195"
     CDS             430..1515
                     /locus_tag="VFC15_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZM11749.1"
                     /translation="MRGAFADQGGLFSYISPEARVPPNHPLRKIRALVRDVLSELNRS
                     LGRLYASEGRPSIPPEQLLSALLLQVFYGIRSERQLMEQLDYNLLYRWFVGLSPDDPV
                     WDPTSFTKNRDRLQNGEVFKKFMTRLLNHPQVKPLLSDEHFSVDGTLIEAWASQKSFR
                     PKDGSGDDDDGANFHGQKRKNDTHASTSDPDSRLYRKAAGREAKLCYMGHATMENRHG
                     LAVAGMVTHANGTAERRASEKMLKVRRKAAGRRITAGEDKAYDTADHVANLRDINVTP
                     HVTQNQAVTKTGRTRNSAIDERTTRHSGYGMSQSRRAMIECIFGWGKQHGTMRKTKHR
                     GIDRVAANFLLNLIAYNLIRIPKLMAA"
     gene            1702..2653
                     /locus_tag="VFC15_16200"
     CDS             join(1702..2046,2048..2653)
                     /locus_tag="VFC15_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611189.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM11750.1"
                     /translation="MGRGRPYSMDLRERVVASVETGGLSCHRAAAQFGVGVNTAILWV
                     RRFRETGSFAPGQMGGHKPKAISGEHRDWLLERTKARDFTLRGLVTELAERGLKVDYR
                     SVWNFVHAENLSFKKSVVASERDRPDVARRRAQWKKYQDQIEPKRLVFIDETWTKTNM
                     ATLRGWAPRGTRLTAKVPHGHWKTTTFLAALRLDRIEAPWVLDGPIDGESFTIYVEKV
                     LLPTLKPGDIVVMDNLGSHKGQSVRALILSVGAKLFFLPKYSPDLNPIEQVFAKLKHL
                     LRKEAARTVEAVWAAIGKLLQAFVPKECANYFKNSGYAST"
BASE COUNT          618 a          837 c          846 g          526 t
ORIGIN      
        1 gatctgcggc gatccggagt gagtttacgg aaaatcgagg ccgcaggtct ggtctcgtca
       61 cgaccgtccg gcgcgtcagc ctcggacagg tgccgcagct ctggaacgat catgctcgcg
      121 ccctccaagc gtctagatac ctaagactac tacgagactc cgtagaatcg accgcgcccc
      181 ccaatccgcc gcgcggtcgc cacgataagg atgtgggagg cgtgttcgaa cgataagcag
      241 ctctccgaca acaacccatc tctgggtgcc gaccggcacg ttcgatcgcg ataagctggc
      301 ggatcaaaga ccgagctccg tcaaacccgg atgatcaaga ggccgaggcc tagcagtctg
      361 ctgaaaaagc tctcgcaaag gcctgcggtt gatgattcaa ttatatatca tgaatcgtcg
      421 ggggctcgca tgcggggcgc ttttgcggat cagggcgggt tgttttcgta catttcgccg
      481 gaggcccggg tgcctcccaa ccaccctctg cggaagatcc gggcgctggt ccgggatgtc
      541 ttgagtgagc tgaaccgtag ccttgggaga ctttacgcca gcgaagggcg tccctcgatc
      601 cctcccgagc aattgctgag cgcattgctg ctgcaggtgt tctacggcat tcggtcggaa
      661 cgccagttga tggagcaatt ggactacaat cttttgtacc gctggtttgt ggggctttcg
      721 cccgacgacc cggtctggga cccgaccagc ttcaccaaga accgcgaccg gctgcagaac
      781 ggcgaggtgt tcaagaagtt catgaccagg cttctgaacc atccgcaggt caagccgctg
      841 ctgtcggacg agcatttctc ggtggatgga acgctgatcg aagcctgggc ttcacagaag
      901 agctttcgcc ccaaggacgg cagcggcgac gatgatgacg gcgcgaactt ccatggccag
      961 aagcgcaaga acgacaccca cgcgagcacc agtgaccccg acagccgact ttatcgtaag
     1021 gcggccgggc gggaggcgaa gctttgctat atgggccatg ccaccatgga gaaccggcac
     1081 gggctggcgg tagccggcat ggtcacccat gccaatggca ccgccgaacg ccgagcctcg
     1141 gagaaaatgt tgaaggtgag acgcaaagct gccggtcgcc gcattacggc aggagaagac
     1201 aaggcgtacg atacggccga tcatgtggcc aatcttcgcg acatcaacgt gacaccgcat
     1261 gtgacgcaga accaggctgt caccaagacc ggcaggaccc gcaacagcgc catcgatgaa
     1321 cgaaccacgc ggcattcggg ttatggcatg tcgcaatccc gccgggcgat gatcgagtgc
     1381 atcttcggct ggggaaagca gcacggtacc atgcgcaaga ccaaacatcg cggcatcgat
     1441 cgcgtcgccg ccaacttcct gctcaatctg atcgcctata acctgatccg catccccaaa
     1501 ctgatggctg cctagctaac caggtctcaa actcctaaac atgccatccc aacaacggtc
     1561 ccgaaaacag aaaaaacgct tcgttcgttg cgtttttcag cagactgtta gagcgggatg
     1621 cggttaggtt gattcgattt gggattccca aatcagtacg gctctgattc aacatgctgg
     1681 ctgggaagga ggccagcatg gatgggaagg gggaggccgt attcgatgga tcttcgcgag
     1741 cgcgtagtag cgtcggttga gacgggcggt ttgtcctgcc accgggcggc ggcccaattt
     1801 ggggtgggcg tcaacacggc cattctgtgg gttcggcgct tccgagagac gggcagcttt
     1861 gcaccgggcc agatgggtgg acacaaaccg aaagcgattt cgggggagca ccgtgattgg
     1921 ctgctggaac gcaccaaggc cagggacttc accttgcgcg ggcttgtcac cgaactggcc
     1981 gagcgcgggc tgaaggtcga ttaccggagt gtctggaact ttgtccacgc cgagaatctg
     2041 agcttcaaaa aaaagcgtcg tcgccagcga gcgcgaccgt cctgacgtcg cccgccggcg
     2101 tgcgcaatgg aagaagtatc aggaccagat tgaacctaag cgcctcgtct tcattgacga
     2161 gacctggacc aagaccaaca tggcgacctt gcggggttgg gcgccacgcg gcacgaggct
     2221 cacagccaag gttccgcacg gccactggaa gaccacaacc ttcctcgccg cgctgcgtct
     2281 cgatcggatc gaggcgccat gggttctcga tggcccgatc gacggtgaga gcttcacaat
     2341 ttacgtcgag aaggttctgc tcccgaccct gaagcccggt gacatcgtcg tcatggacaa
     2401 tctcggcagt cacaaggggc agtccgtacg ggcgctgatc ctatcagttg gcgcaaagct
     2461 cttctttctg ccgaaatatt cgcccgacct gaaccccatc gagcaggtgt tcgccaagct
     2521 caagcacttg cttcgcaaag aagccgcccg aaccgtcgaa gccgtctggg ccgcaatcgg
     2581 taagttgctc caggcgttcg taccaaagga atgcgccaac tacttcaaaa actcaggata
     2641 cgcctcaacc taaaatcatt ccgctctagc gcgcttcgtg gacgctcttg cggaagcgct
     2701 ggatcagccg cagcgtgcgc gatgaccagc tgctgccgtc gccgcgcagc gcgtccctga
     2761 ttctggattt gatcggatcg accagcgcgc tcgccttggc acgcagtcca aggataaact
     2821 catagag
//
LOCUS       DASRKK010000664         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1237676, whole genome shotgun sequence.
ACCESSION   DASRKK010000664 DASRKK010000000
VERSION     DASRKK010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237676"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFC15_16205"
     CDS             <1..623
                     /locus_tag="VFC15_16205"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HZM11751.1"
                     /translation="GTVEAYYQEAGRAGRDGEAAECVLYYSGRDIRTQKFFIDNIGEN
                     NPTLSESELGRLKSLANRKLQLMLAYASTVRCRRSGILAYFGEPRSVSGCICDVCRGT
                     NRPLQVASVDRRARRTTNQTRTADNTTQPLDGSAELRFLALRKTRAELAAVRKLPAFC
                     ILHDKVLREVARTAPADILSFLQIKGIGPAKAEQFGPAFLAALKNG"
     gene            complement(697..1353)
                     /gene="tsf"
                     /locus_tag="VFC15_16210"
     CDS             complement(697..1353)
                     /gene="tsf"
                     /locus_tag="VFC15_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZM11752.1"
                     /translation="MSTTTVNITAAQVKELRDKTNAPMMDCKNALTEAKGDVEAAIVV
                     LRKKGIATAAKKATRATSEGSVASYIHAGGKIGVLVEVNCESDFVARTEDFQQLVHDV
                     AMHIAATDPKFVRKEDVTPEAFEKEKDIYRAQAAATGKPPAVVEKIVEGKMGKFYEEV
                     CLLEQPFIKDQTVTVHQLIASVVGKLGENISVKRFARFKVGDVNETVAVSKAVDQGAE
                     "
     gene            complement(1644..2534)
                     /gene="rpsB"
                     /locus_tag="VFC15_16215"
     CDS             complement(1644..2534)
                     /gene="rpsB"
                     /locus_tag="VFC15_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZM11753.1"
                     /translation="MANITMKELLEAGVHFGHQTKRWNPKMKEYIFGERNGIYIIDLQ
                     KTLKMFKEASKFVQEQAADGKLVLFVGTKRQAQDAIAEEATRCGMFYVNQRWLGGLLT
                     NWITVQKSVKRLKELDEMATDGRYDLLPKKEVVKLERERKHLQANLAGIKNLNRLPDV
                     LFVIDSNKEQIAVKEARKLGIPVVAVVDTNCDPSEVDYVIPGNDDALRAIRLFASKIS
                     DSVIEGAQVVTDKQMADIAAGVQHDEAAAAAAASAADGEAGTAPGLEAAEMEEVSMEE
                     VLGKTARRVAPAEVVETPSA"
     gene            complement(2745..3140)
                     /gene="rpsI"
                     /locus_tag="VFC15_16220"
     CDS             complement(2745..3140)
                     /gene="rpsI"
                     /locus_tag="VFC15_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZM11754.1"
                     /translation="MAELVQYYGTGRRKSSVARVFLRPGSGEFTVNGKPFEVYFVTEA
                     QRIMAKSSLVLTETVGSFNVLCTVDGGGVNGQAGAVRMGIARALQEFKPELRPKLKAE
                     GFLRRDARGKERKKYGQKGARKRFQFSKR"
     gene            complement(3275..3709)
                     /gene="rplM"
                     /locus_tag="VFC15_16225"
     CDS             complement(3275..3709)
                     /gene="rplM"
                     /locus_tag="VFC15_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZM11755.1"
                     /translation="MSTYFPKGEIARKWFVVDASGQTLGRLATRVARILAGKNSPQYT
                     PFLDTGDHVIVINAEKVKISGLKADTKTYYHYTGYPGGIRGEEYKKRFDRKPTLIVED
                     AIKGMLPHTKLGRAMFSKLKVYKGDKHPHVAQKPQTLEAVAK"
BASE COUNT          776 a         1264 c         1042 g          910 t
ORIGIN      
        1 ccgggacggt cgaggcctac taccaggagg ccggccgtgc tggtcgcgat ggcgaagctg
       61 cggagtgcgt gctctattac agcggacgcg atattcgcac tcagaagttc ttcatcgata
      121 acattggcga gaataatccc acactgtcgg agtcagaact cggccgcctg aaatctctcg
      181 ccaatcgcaa gcttcagttg atgctggcct acgcttctac tgttcgttgc cgccgcagcg
      241 gcatcctggc ttacttcggc gaaccgcgct cggtttccgg atgcatctgc gatgtgtgcc
      301 gcggcactaa ccgaccgttg caggttgcgt ctgttgaccg ccgtgcccgc cgcactacga
      361 accaaacccg caccgcggac aacactaccc aaccactcga cggcagcgcc gagttgcggt
      421 ttctcgcctt gcgaaagacg cgagcggaat tggcggccgt gcgaaagttg ccagccttct
      481 gtatcctgca tgacaaagtt ttgcgcgaag tcgcccgcac tgcgccggcg gatatactgt
      541 cgttcctgca aatcaaagga attggcccag cgaaggccga gcaattcggc ccagcgttcc
      601 tggcggctct taagaacgga tgagccttgg ttgcttcccg cccaatagca aagggcggga
      661 atgaatcccg ccctctaggt ttcctgctga attgacttac tcggcgccct ggtccacggc
      721 cttgctgacc gcgaccgtct cgttcacgtc tcccaccttg aagcgggcaa agcgcttgac
      781 cgagatgttc tcgcccagct tgccaacgac gctggcgatc aactgatgca ccgtcaccgt
      841 ctggtccttg atgaaaggct gctccaacag gcagacttcc tcatagaact tgcccatctt
      901 gccttcgacg atcttctcca ccaccgcggg cggtttgccg gttgcggcgg cctgcgcgcg
      961 atagatgtcc ttctctttct cgaaggcctc cggcgtcacg tcctccttgc gaacaaactt
     1021 cgggtcggtg gcagcgatgt gcatggcaac gtcgtgcacc agctgctgga agtcctctgt
     1081 gcgggccaca aagtcgctct cgcagttgac ttccaccagc acgccaatct tgcctccggc
     1141 gtggatgtag ctggccaccg agccttcgct ggtggcgcgg gtcgccttct tcgcagccgt
     1201 cgcaatgccc ttcttgcgca gcacgacgat agcggcctcg acgtcgccct tggcttccgt
     1261 cagggcgttc ttgcagtcca tcatgggagc gttcgtcttg tcgcgaagct ctttcacttg
     1321 agcggcagta atgttcacag ttgtagtact catttcttag tattccctct gggctgggag
     1381 gagcaattcg ctttagcagt caacaatcag ctcaaagccg tttgctgtct gctattcgac
     1441 aactcctctt agctcgctga aacagccctc taaaaagatt agatgattca aactcagtaa
     1501 cgtctcaccg tcagaatgct tggtgcgtgc gcgtgcgcca gcccaaagct tattgcttat
     1561 tactaattac ttcttcacaa aacgacccgc gccttgtagc tgcgcgggtc ttgaaaacca
     1621 cttcgtcagc ctctcggctg cgattacgcg cttggagtct cgaccacttc tgccggggcg
     1681 acacggcgtg ccgtcttgcc caggacttcc tccatgctga cttcttccat ttcggcggcc
     1741 tccaagcccg gcgcggtacc agcttcgccg tccgcggcac tggcagccgc cgcggcagcg
     1801 gcttcatcgt gctgcacccc cgctgcgata tcagccatct gcttgtcggt cacgacctgc
     1861 gcaccctcga tcaccgaatc ggaaatcttt gacgcaaaca accggatcgc tcgcaacgca
     1921 tcgtcattgc ctgggatcac gtagtccact tcgctcggat cgcaattcgt atcaaccacc
     1981 gcgaccaccg gaatacccag cttgcgggct tccttcaccg cgatctgctc tttgttcgag
     2041 tcgatcacga agagcacgtc aggcaaccgg ttcaggttct tgatgcccgc caggttcgcc
     2101 tgcaggtgct tacgctcgcg ttccagtttc accacttcct tcttcggaag cagatcgtaa
     2161 cggccgtcgg tggccatctc gtcgagttcc ttcagccgct tcaccgactt ctgcaccgtg
     2221 atccagttgg taagcaatcc gcccagccag cgctggttga catagaacat gccgcagcgt
     2281 gtcgcttctt ccgcgatggc gtcctgcgcc tgccgcttgg tgcccacaaa cagcaccaac
     2341 ttgccgtccg ccgcctgctc ctgcacgaac ttcgacgctt ccttgaacat cttcagcgtc
     2401 ttctgcagat cgataatgta aatcccgttg cgctcgccga agatgtattc cttcatcttc
     2461 gggttccaac gtttcgtctg atgcccgaag tgaacgccgg cttcgagcaa ctccttcatc
     2521 gtgatatttg ccaagctacc tcctagcttg catcctttgc ggcgtacctc aacgcattgc
     2581 gcctcggccg gatcgctccg gattacattc ccgttacacc cgccccgttg aaacctatag
     2641 acagcgggtg cgagaagatt cgttccggtt cacttgctgc ctcccgcccc cggaaccaca
     2701 aagcgagagt agctgaacac cagttaccaa ttaccggcca ccagttacct cttggagaac
     2761 tggaagcgct tgcgggcgcc cttctggccg tacttcttgc gctctttgcc gcgcgcgtcg
     2821 cgacgcagaa agccttcggc cttcagcttg ggacgcagtt ccggcttgaa ctcctgcaag
     2881 gcacgggcaa tacccatgcg gaccgcgcca gcctgcccat tcacgccacc accgtccacc
     2941 gtgcacagca cgttgaagct gccaacggtt tcggtgagca ccagcgaaga cttcgccatg
     3001 atgcgctggg cctcggtcac gaaatacacc tcgaacggct tgccgttgac cgtaaactcg
     3061 ccgctgccag ggcgaagaaa cacgcgggct acgctcgatt tgcgccgtcc cgtaccgtaa
     3121 tattgaacca gttctgccat ttcgtatccg tcgggagcaa gctcccttaa tccttccaaa
     3181 aaagttctga ctgggtgccc cattctatcg accgttttgt ggtctatagg gtggggtcga
     3241 aaatgtacga cccgaccaca tggtggttct tttcctactt cgcgaccgct tctaacgtct
     3301 gtggcttttg cgccacatgc ggatgcttgt cgcccttata aaccttcagc ttagagaaca
     3361 tggcgcgtcc cagcttggtg tggggaagca tgcctttgat ggcgtcttcc acaatcagtg
     3421 tcggcttgcg gtcgaagcgc ttcttgtact cttcgcccct gatgccgccg gggtatccgg
     3481 tgtagtgata ataggttttc gtatccgcct tcaggccaga aatcttgacc ttctcggcgt
     3541 tgatcacgat cacgtgatcg ccggtatcga gaaagggcgt gtactgcgga ctgttcttac
     3601 ccgcgaggat gcgcgccaca cgtgtcgcca gccggcccag tgtctggccc gacgcatcga
     3661 caacaaacca tttacgcgca atttcccctt tggggaaata ggttgacatc tgaaatctcc
     3721 cagaattgtt agaaaaaccg cgaaagagcc gctgagttcg tgacgctgga cgcgcaggga
     3781 accaccaggc gggcaaacgc agaactgcca aagacctaag aataaacgag tcacgcgccg
     3841 tctgtcaacg cagagtctct tccaatacaa gatgagcctg aaggcgaagt cagtttccaa
     3901 tacaagatga gcctgggacg cgtggcatgc tgaggattga caaccagtgt gcacgatgca
     3961 gagaaactaa gcctggatca gatcgaggct tt
//
LOCUS       DASRKK010000665         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_65277, whole genome shotgun sequence.
ACCESSION   DASRKK010000665 DASRKK010000000
VERSION     DASRKK010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65277"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(472..1926)
                     /locus_tag="VFC15_16230"
     CDS             complement(472..1926)
                     /locus_tag="VFC15_16230"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM11756.1"
                     /translation="MAKHKQSTLERLRNGKLPCNRKQRRELERRLNSADPGLEIINPH
                     AAGIDVGNESHYVAVPAGRDARPVREFGSWTAALEEMAQWLKSCGVETVVMQSTGVYW
                     IAVHDVLERHGLEVNLVDARGTKSVPGRKSDVQECQWLLKLHTYGLLRSCFLPAPEIH
                     GVRTVWRLRDQHVKDAGRCIQHMQKALIEMNVQLHIAISDLSGVTGQAIIRALLSGQR
                     DPRALASLRHWRIQATEEEIVHSLQGNWKEDALFELQQAVEAYDFYRKQMAACDTQLK
                     SYMAALPTREAVPTTPAASEPAVAVDGRKKRRPRKPKGNQPAFDLAEELERILGVNAK
                     RIDGIDVMTIQTVLAEVGPDLSAWKTEDQWSSWLNLAPKRDISGGRVIRHRREYRTNR
                     AGNAFRMAAESLVRSQSYLGARYRYLRAKLGGLKAVKAMARVLACLYYRLVTKGQVWV
                     DRGTAEFEQRRQQHDLATLQRKARSVGMQLVPTT"
BASE COUNT          489 a          770 c          706 g          588 t
ORIGIN      
        1 gagtattcct ccgtgttgta cttttgaaac ctaaaagtct agatcacgaa cattgcgaac
       61 ggtcctcggc gatcgcaccg agttttgctc cgaggaagtt cgttttgcac cggatctcaa
      121 atcgactggc accgatcaag aacttcgtgt gcatgccgcg ggagagttgg ctggatactg
      181 gcccgcaggg acaagttcgg ctgtgatccg tcgtggccac gctggggatc acgaccgaag
      241 caaggagaaa ccggcagttg tcggcgctca aaacagcgac ccacactgga ggctagcccc
      301 ggcagttgtc cagaagtctt ctctccggaa acttcatctt tgctgacaac gcaaggggcg
      361 ggagttgcct ttcgatagaa gctggggcgg tgatctgggt caatacttct cccgcttcct
      421 ttgtggagag ttgttttggc cgcgacgctt tggaggttta atcgtttagg tttacgttgt
      481 cggcactagt tgcatcccca cgctgcgggc tttgcgctgc agggtggcga ggtcatgttg
      541 ctgcctgcgc tgctcaaatt ccgcggtgcc gcggtcaacc caaacctgcc ccttggtaac
      601 cagccgatag tataagcacg ccaagacgcg ggccatcgcc ttgacggctt tcagtccccc
      661 caacttggca cgcaggtatc ggtatcttgc acccaaatag ctctggctgc gtactaacga
      721 ttcagcggcc atgcgaaagg cattgccggc tcgattggtg cggtattcac gcctatgacg
      781 aatcactcgg ccaccgctta tgtcacgctt gggagctagg ttcagccacg agctccattg
      841 gtcttcggtc ttccaggcac tcaggtcggg ccccacttcg gccagtacgg tctgtatcgt
      901 catcacatcg atgccgtcga tccgcttggc gttgactcct agaatgcgct ccagttcctc
      961 cgccagatca aaggccggct ggttgccttt gggtttgcgt ggacggcgtt tcttgcgccc
     1021 gtccacagct accgccggct cgctggccgc cggcgtcgtg ggaaccgctt cccgggtagg
     1081 cagtgcggcc atgtacgact tcagctgagt gtcgcatgca gccatttgct tccggtaaaa
     1141 atcgtaggcc tcgacggctt gctgcaattc gaacaaggcg tcctctttcc aattcccttg
     1201 caggctgtgg acgatctctt cttctgtggc ttgaatgcgc cagtggcgca gggacgccaa
     1261 ggcccgcggg tcgcgttggc cgctcagcag ggcgcgaata atagcctgtc cggtcactcc
     1321 gctgagatcg ctgatcgcga tgtgcaattg cacgttcatc tcaatcagcg ccttctgcat
     1381 atgctggata cagcgccctg catccttgac gtgctgatcc cgcagcctcc acaccgtacg
     1441 caccccgtgg atctcgggcg ccggcagaaa gcaactccgc agcagaccat aggtgtgcaa
     1501 tttcaaaagc cactgacatt cctgtacgtc actcttgcgc ccaggcacac tcttggtgcc
     1561 acgggcatca accaggttga cttccaagcc gtggcgttcg agcacatcat gcacggcgat
     1621 ccagtacacg cccgtggatt gcatcactac ggtttccaca ccacagcttt tcaaccactg
     1681 cgccatctct tccagggctg ctgtccagga tccgaactcg cgtaccggtc gcgcatcgcg
     1741 tcccgccggt accgccacgt agtggctttc gttgcccacg tcaataccgg cggcatgggg
     1801 attgataatc tccagtcccg gatccgcgga gttcaagcga cgttccagtt ctctccgctg
     1861 cttgcggttg cacggcaact ttccattgcg tagtcgctcc aaggtagatt gcttgtgctt
     1921 ggccatggca tactctccct attgtttaaa ctggccaagc tcggctcggt tggcgattac
     1981 gacacaggct ctccggcggg gtcgagccga gccttttgca aggccaactc gccaccaaac
     2041 aatggaccgc agcaaccgaa gtcagacttg tggacgggca cgaaggcacc aagaagattg
     2101 cgaccatagc tgtcgagctt ggcttccctg attgtgtaat agtccttccc gctcgcgcaa
     2161 agtttccggc actatggacc cgcaagaggc gcgcccaaaa cttgtgggaa gttggatggg
     2221 ctcgcctagt caattcaacc aggggcgctg ctcagcgata attttgcggc ttgcccaatt
     2281 acccttgtga atccgccgca ctaagtgctt gatattggag cgcgtcaggt cgcgctgttc
     2341 ggctgcccac aaacagacgg ccccaatgct atggaagccc tcgcgaatgg catacgccga
     2401 cgccaaaaac ctcgtcagtg aatgacagtc gggaccgcag aatctcagct gagcgcgttt
     2461 ttcaatttcg gtcatccagc caggatgaga taagttatgt ctacttcacg gcgtcagcgg
     2521 ttttgtcggc gcctttcttg acggcctttc cgg
//
LOCUS       DASRKK010000666         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1434171, whole genome shotgun sequence.
ACCESSION   DASRKK010000666 DASRKK010000000
VERSION     DASRKK010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434171"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(527..1705)
                     /locus_tag="VFC15_16235"
     CDS             complement(527..1705)
                     /locus_tag="VFC15_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11757.1"
                     /translation="MSWRPIVSARSVCGLAFVFALLAPPVFADTNKDTHELLSKSFQQ
                     ADLWKQGPVKLAAKVRRYTASAQAQNLEYTVYWAGPERWRAEWSGPDVQQITILNNGK
                     LSYVTSTPELLWSGVELEAALAALDGGTPAGPYRLAPLAYENAKLHLSTKKINGIEAR
                     CLAFGKPKSTLCMDPSSGHLLTAENDLGSFAYSDYTTIGSNSYPQTVKVGSVHTSYEG
                     DDYVWVGDGISYPRNLKEVHVKAPIEQAEVTVTRGEQFPDSLFVAPENATTADFASCA
                     APASNFTAPHLDKSVKAKRPDAARKNYRYGWVWVLATVGKDGSVQKTTMLSGDPLLSP
                     GATNAVQRYKYISYMRCGEATEFQQLVVVGFPPPPPPVYTEHSNERATYPTCFPCAPN
                     "
     gene            complement(1947..2444)
                     /locus_tag="VFC15_16240"
     CDS             complement(1947..2444)
                     /locus_tag="VFC15_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZM11758.1"
                     /translation="MLQKRIDDQPTISREIVREAEAIFHGHLKRVGLKQTGQRGVILR
                     TFLDTRDHLSTDELYRLVKKHDERIGFTTVYRTLKLLAECGLASEVAFNDGVTRYEQQ
                     YNRRNHHHMVCTECGSSVEFFSRDIERIEQEVGRKNKYLPTRHTFQIYGVCERCQKKQ
                     KRSLS"
     gene            complement(2600..3997)
                     /locus_tag="VFC15_16245"
     CDS             complement(2600..3997)
                     /locus_tag="VFC15_16245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZM11759.1"
                     /translation="MTTPQQYDAIVIGSGQAGTPLSTALAEAGMHTALIEREHVGGTC
                     VNEGCTPTKTMVASARVAYLARRGADYGVHTGDIRIDMERVRQRRRDIVNSFRNGSQG
                     RIEKTANLELIFGEASFTGAKSLLVRQKDGSQLALTAEKIFINAGARPSVPALDGLKA
                     VSFLDSTSIMELDTVPEHLLVLGGGYVGLEFGQMFRRFGSRVTIVQSAGQLLNREDPD
                     IAKKVAKILQQDGVEVLLNATANRVSQSDGSIRLEIQQQGRSTTLAGSHLLVATGRMP
                     NSDTLHLAAAGVQTDDHGFIEVNGRLETTSNGVYALGDIKGGPAFTHISYDDFRIIRT
                     NLIEKGSATTDGRLVPYTVFIDPQLGRIGITETEALVQKRNIRVAKLPMTSVARALEV
                     DETRGFMKAIVDGESNQLLGAAVLGIEGGEIMSAIEIAMMGRLPYTALRDGVFAHPTL
                     AESLNNLFMAMDSGK"
BASE COUNT          907 a         1292 c         1321 g          929 t
ORIGIN      
        1 gaaagtcgtc actgggcaaa acgaagacta catgggcctt ggcccggtat tcgtgggcca
       61 gttcgaggcc aaagttcaca gcaacgctgg agtagcggga aaagtccgtc gcgatgagca
      121 cactgtggat gcccaccccg attgggttga gctgtgccgt caccatcttg cgcgaccatt
      181 ctccccacgc ggtggggtcg ttggatggaa gctgtctcgc ccaggcgcga agccgcgccc
      241 cgccaatcat cccagcagga tgcttccatg cactcgtcta tctcttttgt agcgcaatgc
      301 gggccagaac gatgtgacgt gcgtcaaccc ggaaggtgat gcagcgcagt tttgggatag
      361 ggccgtttat gagatggatg aaggagagat gatcacttgt gtaatggcga tgttacgtaa
      421 gtagtcgcta aggttgcctt ccgaaaaacc ctaagccttg ctcgagattg gacttaggta
      481 ggcgtcattc atagatggcc gttgaatcag tccgcgccat tactccttaa ttcggcgcgc
      541 aggggaaaca agttggataa gtagcccgtt cattggagtg ttccgtataa acgggcggcg
      601 ggggcggagg gaagccgacc acgaccagct gctgaaactc cgtcgcctca ccacagcgca
      661 tataagaaat gtatttgtac cgctggacgg cgttcgtggc cccggggctc aacaaaggat
      721 cgccgctcaa catcgtagtc ttctgcacag atccatcctt gccaacagtc gccagcaccc
      781 acacccagcc gtacctgtaa tttttgcggg cggcgtcggg cctcttcgcc ttcaccgact
      841 tgtcgaggtg gggcgctgtg aaattgctgg cgggggcggc gcaggaggcg aaatccgcgg
      901 tggtggcgtt ttcgggcgcg acgaagaggg aatctgggaa ttgctcacca cgggtgacag
      961 tcacctcggc ctgttctatc ggggccttta cgtggacctc cttcaagttt cgcggatagg
     1021 agatgccgtc gccgacccag acgtagtcat cgccctcata actcgtgtgt acggagccga
     1081 ccttcaccgt ctgcggatac gaattgctgc cgattgtggt gtagtcgctg tacgcgaagc
     1141 tgcccaggtc gttctcggcg gtgaggagat gtccgctgga tgggtccatg caaagcgtgc
     1201 tcttcggctt gccgaacgcc aggcaacggg cttcgatccc gttgatcttc ttcgtggaga
     1261 ggtggagctt ggcgttctca taagcgagtg gtgccaggcg atacggtccg gcgggcgtgc
     1321 caccgtccag cgcggcaagg gcagcttcga gctcaacgcc agaccacagc agctccggtg
     1381 tactggtgac gtatgacaat ttgccattgt tgaggatggt gatctgctgc acgtccggac
     1441 cgctccactc cgcacgccac ctctccgggc cagcccagta gacggtgtat tccagattct
     1501 gcgcctgtgc gctggcggtg tacctgcgga ccttcgccgc cagcttgacc ggcccctgct
     1561 tccagagatc ggcttgttga aatgacttgg aaagcagctc gtgggtgtcc ttgttcgtgt
     1621 cggcaaagac cggcggagcg agcagggcaa atacgaaggc caagccgcaa acgctacgag
     1681 ctgagacaat tggtcgccaa ctcacggatc gccttccttg aggagcattc aattctaggt
     1741 acaagcgacg tgcacagtgc gttaagtcgg gtgaaattgt gtaaagtttt gttcgcatcg
     1801 ggacgagatt cttgcggccc aatggctgat agttgacgta atcaggcctt atcacaagca
     1861 atcggcgatt gggcctgcaa agggtcgcat gtgcgcggtc ggcgctccat gcacaaaaga
     1921 ccctcttcca tgccccattt gtgacctcac gacaagctgc gcttctgttt cttctggcag
     1981 cgctcgcaga cgccgtaaat ctggaaggta tggcgggtcg gcaggtactt gttcttgcgg
     2041 ccaacctcct gctcaatgcg ctcgatgtcg cgcgagaaga actccaccga gctaccgcat
     2101 tcggtgcaga ccatgtgatg atggttgcgg cgattgtact gctgctcata acgggtcacg
     2161 ccgtcattga aagcaacttc gctggctagc ccgcactcgg ccagcagctt caaagttcga
     2221 tagacagtgg taaatccgat gcgctcgtcg tgtttcttga ccagccggta cagttcgtct
     2281 gtggagagat ggtcacgtgt gtcgagaaac gtgcgcagga tgacaccccg ctgccccgtc
     2341 tgcttgaggc ccacgcgctt gagatgccca tggaaaatcg cctcagcctc gcgcactatc
     2401 tcgcgcgaga tggttggctg atcgtcaatt cgtttttgaa gcatggggct tgccattccc
     2461 cagtttgggg cctgtccact acgcaaccgc cgcgcaactt tctatcagca taacaccgtc
     2521 aacgaggcgc acgtgaccgt ctattgggcg aaatcaaccg ggcgggcgct cccagtcaat
     2581 gtatccacgg cagggccttc tacttgccgc tatccattgc catgaacaag ttgttcagcg
     2641 attccgccag agtagggtga gcgaagacgc catcgcgcaa ggcggtgtag ggcagccttc
     2701 ccatcatcgc aatctcgatc gccgacataa tttcgccacc ctcgatgccc agcaccgcgg
     2761 cgcccaggag ctggttactt tcgccgtcca caatggcttt catgaaaccg cgggtctcgt
     2821 ccacctcaag ggcgcgggcg acggaggtca tcggcagctt agccacgcga atgttccgct
     2881 tctgcacgag agcttcagtt tcggtgatac caatgcggcc gagttgcgga tcgataaaga
     2941 cggtgtaagg cacgagccgc ccgtcggtgg tagcgcttcc cttctcgatg aggttcgtgc
     3001 ggatgatgcg aaagtcgtcg tacgagatat gcgtaaacgc cggcccaccc ttgatgtcac
     3061 cgagtgcata gacgccattc gacgtagttt cgagacggcc gttcacctcg atgaatccgt
     3121 gatcgtcggt ttgcaccccc gccgccgcaa gatgcagggt gtccgagttc ggcattcgcc
     3181 cagtggcaac cagcagatgt gaaccggcaa gagtggtcga acggccttgc tgctggatct
     3241 ccagtcgaat gctcccgtcc gactggctca cgcgattcgc agtcgcgttc aacaagacct
     3301 caactccgtc ctgctgcagt atcttggcga cttttttcgc gatatcgggg tcctcgcgat
     3361 tgagcagttg ccccgcggac tggacgatgg tcacccgact accgaagcgg cgaaacatct
     3421 gcccaaactc caggccgacg tagccgcctc caagaaccag cagatgctcc ggaaccgtgt
     3481 ccaactccat gatggaagtg ctgtcaagaa aggatacggc cttcaggccg tcgagggcag
     3541 gcacagaggg acgcgcacct gcgttgatga agatcttctc cgcagtgagt gcaagttggc
     3601 tgccgtcttt ctgacggacg agcaaggact tggcgccggt gaaactggct tcgccgaaga
     3661 tcaactcaag gttcgcagtc ttttcgatgc gtccctggct gccattgcga aagctgttca
     3721 cgatatcgcg cctgcgctgt cgaacacgtt ccatgtcgat acggatgtct ccggtgtgaa
     3781 cgccgtaatc agcgcctcgc cgcgccagat acgcgacgcg ggcgctggcg accatggtct
     3841 tcgtgggagt gcatccctca ttgacgcagg taccgccgac atgttcccgc tcgatgagag
     3901 cggtgtgcat cccggcttca gccagggccg tggaaagagg tgtaccggcc tgtcctgagc
     3961 cgataacaat ggcatcgtat tgctgcggag tggtcatagg gacgccccga ttgaatgtac
     4021 ctcaactctg gaatgagtga gtcgctagat cgatttcgga attagtgtat ccggcagggc
     4081 gggtagtgcg gcgcttcagc gccgcgttcg ccgcgaacca caaaaccgcg gcgggcgttg
     4141 cgctcatcgc gatgagcgca acgcctgccg cccccacggc ttgtaccggt ggacgccgga
     4201 ccgaaaggcc ggctccaccc ggactacacc aattctgaaa acgccataga gatgctgagc
     4261 ctgtcgggaa gagagtctca ggctcctgga ataaatcgac cgacttcaga atcgcgaaac
     4321 tagaagcagc cccaatccca ctgcgatcag atcctccggg acagcgatag caaaatccgg
     4381 aacgtgcaag gcgcgtacta ggccgacgcg agcgcgataa ccgccaaacg ctcccgcaat
     4441 tgcgccgat
//
LOCUS       DASRKK010000667         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1237926, whole genome shotgun sequence.
ACCESSION   DASRKK010000667 DASRKK010000000
VERSION     DASRKK010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237926"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..133
                     /locus_tag="VFC15_16250"
     tRNA            59..133
                     /locus_tag="VFC15_16250"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91..93,aa:Asn,seq:gtt)
     gene            complement(247..1617)
                     /locus_tag="VFC15_16255"
     CDS             complement(247..1617)
                     /locus_tag="VFC15_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11760.1"
                     /translation="MKESKLGFRPGQPDPVRERAWREVEAALNDQANTMRVAVDKQMM
                     SRAQAPKPTAAKPEARPASRGGGRIFSRKGSSLLWCAYYLRGREYRESTHETDPQKAE
                     KFLKRRLKAVGADQIGAAPFIGPHQERVRISELLDALQADYQLREKDSPQFRSSLKYV
                     RLAFGDWRAVEVTAERVDEYVKEQLGADLKPASINRRTQLLKQAYKLAIERGHLSKAP
                     IIRHLSEKGNTRTGFFDEQQFREVLTHLPDHLRDCCLFGFLCGWRKNEIKTLAWEDVE
                     GDTIRLKGINAKTGEPRMVVLEGELAELMVRRKAARPVTTATGDVMLADLVFHHQGQP
                     IGDFRKAWQTACVTAGVGKFVCRQCGQPANGHKCESCGTDSIRYVGRLFHDFRRTAVR
                     NMVRAGVPERIAMSVSGHKTRSIFDRYNIVSEDDLRSAMQKTQEFLKGVREQERPKVI
                     IKSAKK"
     gene            complement(1822..2151)
                     /locus_tag="VFC15_16260"
     CDS             complement(1822..2151)
                     /locus_tag="VFC15_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11761.1"
                     /translation="MRNELQIVLMTAQELPKDELPALLGELEQVRCTALARLTSAPVA
                     PQESDELLNVTAAAGRLGTSRDYLYRHHRDFPFTRRLGRKLLFSAAGIEKHIRQTNVL
                     TPRRQKH"
     gene            complement(2270..>2565)
                     /locus_tag="VFC15_16265"
     CDS             complement(2270..>2565)
                     /locus_tag="VFC15_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11762.1"
                     /translation="ISYPDVTTEGRATCEKSVVHPNPPEKQQFHQILLTSTREDPPMM
                     LIERVERVLCGSVPSAQVSQAQRGLEAQRERIPWRDELRPAWKGNGMLPMEGK"
BASE COUNT          440 a          780 c          705 g          640 t
ORIGIN      
        1 cgtaggtgcc gctacaatac agctacaact tgacgcgctg ttggcagcgc acagacactc
       61 ctcagtagct caatggcaga gcatccggct gttaaccgga gggttgtagg ttcgagtcct
      121 acctgaggag ccattctttg cctgcaaatg caatgacttg cagcctggcc ggaaaacgcg
      181 ggcgcgtttt ctcgtgccga atgtccgtgt tttgtccgtg tttccgctac acccaacatt
      241 tcgacctcac ttcttggccg acttgatgat gacctttggc cgctcttgct cccgaacgcc
      301 cttcaagaac tcttgcgtct tttgcatcgc gctcctcagg tcatcctcgc ttacgatgtt
      361 gtacctgtcg aaaatcgagc gggtcttgtg gccgctcacg gacatggcta tgcgctcggg
      421 aacaccggcg cggaccatgt tccttactgc tgttcttcgg aaatcgtgaa aaagcctgcc
      481 cacgtagcga attgagtctg tgccgcatga ttcgcacttg tgcccgttcg caggctgtcc
      541 gcactgacgg cagacaaact tgcccacgcc agccgtgacg caagccgttt gccatgcctt
      601 tcggaagtct ccgatcggtt gcccctgatg atggaacacc aagtccgcaa gcataacgtc
      661 gcccgtggct gtcgttactg gcctggcggc ctttcggcgt accatgagtt ccgccaactc
      721 gccttcgagc acgaccatgc ggggctcgcc ggtcttcgca ttgatgccct tcaaccggat
      781 ggtgtcgccc tcgacatctt cccatgcgag tgtcttgatc tcatttttgc gccagccaca
      841 caagaagccg aagaggcagc aatcccgcaa gtggtcgggc aagtgtgtca gtacctcccg
      901 gaactgctgt tcgtcgaaga atcctgtcct agtgttcccc ttctcggaca aatgccggat
      961 gatgggagct ttggacaaat gtccgcgttc aattgccagc ttgtacgctt gcttgaggag
     1021 ttgcgttctg cggttgatgc tggccggttt taggtctgct ccgagctgtt ctttgacgta
     1081 ctcgtccacc ctttccgccg tgacttccac ggcgcgccaa tccccgaagg cgagacggac
     1141 atacttcaaa ctgcttcgga attgcgggct atccttctcg cgcagttggt agtcagcctg
     1201 gagcgcgtcc aatagctctg agatacgcac gcgctcctgg tgtggtccga tgaacggcgc
     1261 cgcgccgatc tggtcggctc ctaccgcttt caacctgcgc ttcaagaact tctcagcctt
     1321 ctgcggatct gtttcgtgtg tgctctcgcg gtattctctc ccgcgcaaat aatatgcgca
     1381 ccacaggaga gaggaaccct tgcgggagaa gatgcgtccg ccgcctctgc tggccggcct
     1441 ggcttctggt ttagctgcgg ttggtttcgg ggcctgggct cgcgacatca tttgtttgtc
     1501 cacggcgacg cgcatggtat tcgcctggtc gttaagtgcg gcctccactt cccgccatgc
     1561 cctctcacgg acggggtcgg gttgtcctgg acgaaaaccc aacttgctct ctttcatttg
     1621 tggcctccct tcgtgaagaa ggctcttagc gcccggataa cgagctcttg ctgggtgcaa
     1681 tcagcgtcaa cggctgcgtg tttgacctgc cgagacaatt cccgcggcag ccgcaatgtc
     1741 attctcgaag tttcctcgcc gtgtttctct tgtttcatgt ttgattctcc tgggccgtgc
     1801 gtatgcacga cgtaggacag tttagtgctt ttgacgtctc ggcgtcaata catttgtttg
     1861 ccgtatatgc ttttcgatcc ccgcagcgga aaagagtagc ttgcgcccga ggcgtcgcgt
     1921 gaaggggaag tcccgatgat gccgatacag gtaatccctg gatgttccca gccggccagc
     1981 cgctgctgtg acgtttagca actcgtccga ctcctgcggc gcgacgggag ctgacgtcag
     2041 ccgcgccagg gccgtgcagc gcacctgttc gagctcgcca agcagggccg gaagttcgtc
     2101 cttcggcaac tcttgggcgg tcatcagcac gatttggagt tcgtttcgca ttgggtactc
     2161 cacctgatgt gccttcgcaa atccttactg ttcaatcagc tacctgtgcc tgtccgccgt
     2221 ttgtacgcct acttatgccc ctattccctc gcattttgca ggcgaaccgt catttcccct
     2281 ccatcggcaa catcccgttg cccttccacg ccggacgcaa ctcatcacgc caggggatcc
     2341 gctcgcgctg cgcttccaga ccgcgctgcg cttgcgagac ctgggccgaa ggcactgacc
     2401 cgcacaagac gcgctcgact cgctcgatca gcatcattgg ggggtcctcg cgtgtggaag
     2461 taagcaagat ttggtggaat tgctgctttt caggagggtt ggggtgaacg acggattttt
     2521 cgcacgtcgc tcgcccctcg gttgttacat cggggtacga tatat
//
LOCUS       DASRKK010000668        11508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_358540, whole genome shotgun sequence.
ACCESSION   DASRKK010000668 DASRKK010000000
VERSION     DASRKK010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358540"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1268
                     /locus_tag="VFC15_16270"
     CDS             join(67..331,331..1268)
                     /locus_tag="VFC15_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558229.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM11763.1"
                     /translation="MARKKHSPEQIVALLRQIEVAIASGKLTPQACREAGIKEQSYYR
                     WRKEYGGLKLEQARRLKELEKENSRLRRLVTELSLEKQVLKDVAFGKLLSPERRRCAV
                     EHAHQQYGFSERHACRLLGQWRGTQRYLPTQHHDEDGLTRAILQLASQYGRYGYRRIT
                     VELGKAGWCVGKDRVQRIWRREGLRVPQKQRPRGRLWLNDGSCVRLRPERENHVWSYD
                     FVSASTHDGRTLRLLTLIDEYSRKCLAIRVARRLNSYDLIETLADVMLVNGVPEHIRS
                     DNGPEFVAKQMRQWLAGVGSKTLYIEPGSPWENGYCESFNGKLRDECLNGEIFYSLKE
                     AQVVIEQWRVHYNTQRPHSSLGYRPPAPVAILAKQPGHGDVENATRFPHPHTPDYDYG
                     QISNEALH"
     gene            1547..2431
                     /locus_tag="VFC15_16275"
     CDS             1547..2431
                     /locus_tag="VFC15_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11764.1"
                     /translation="MTHLRQLMLEELRRRNFADTTIRTYLHGVEHFSRYFDRQPDKLG
                     PEHIRQYQAALFTKFKFSSNTVGLRLATLRFFYIQVLKRNWSIAETPYPKKVFHLPQI
                     LSQEEVARLIDAAEHPFHRILLMTLYATGARRAEAAHLKVSDIDSKRMVVHIREGKGG
                     KDRDVMLSPKLLEELRTYWRGLRRKPTDWLFPGNRWHTGSHPVTTKVLWTACQNAAQR
                     AGLAHKHIHPHTLRHCFATHLLEAGADLRTIQMLLGHRDLEETTIYLHLSQRHLSATA
                     SPLDALTFTARGEQKHSS"
     gene            2428..3621
                     /locus_tag="VFC15_16280"
     CDS             2428..3621
                     /locus_tag="VFC15_16280"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HZM11765.1"
                     /translation="MSRPPLEVADIVRCAGQSFLENSRRWMNWQHQKVLLAITRCRTA
                     ALGGHRDRCTGCGRTTAISYNSCRNRHCPRCQGNARLRWLQARERELLPTHYGHAVFT
                     LPRELAPLALQNKKVIYDLLFHASAETLLEIAHDPRHLGAEIGFFSVLHTWNQRLQHH
                     PHVHCVLPAGGLAPDHSRWISSRRSFFLPIKVLSRVFRGKFVAGLRSAFLTGTLQFHG
                     RLLPLAEPRAFAAWLRVLFRHDWVVYSKRPFGGPENVLRYLGAYTHRVAISNRRLVAL
                     AEKDVTFRWRDSAHGNKKRSMTLPVEEFLRRFLLHVLPKGFVRIRNFGFLANRQRATL
                     LPLCFQLLGDSESRPTSAPLPSTDQPRSLWNCPACGGTMHVVERLSAAQLLLRSPPEL
                     EQCAA"
     gene            complement(4174..4965)
                     /gene="otsB"
                     /locus_tag="VFC15_16285"
     CDS             complement(4174..4965)
                     /gene="otsB"
                     /locus_tag="VFC15_16285"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZM11766.1"
                     /translation="MWLSRTANPIVVEQFMNAVAQSPASALLLDYDGTLAPFCLNRQE
                     ALPYPEVTALLQQIIDNGRTRVVIITGRNAREVVPLLTVHPSLEIWGCHGLERLRPDG
                     TYETPSVDEPVRHALADADRWLRYQGLHHHAEFKTGAVAIHLRGLDEVTAAEARNQVL
                     LGWLPIAQNTPMELLEFDIGIEMRMPGRDKGDAVRTLLDEIGPEDPVAYLGDDATDER
                     AFLALGIRGLSVLVRPEWRTTAAALWLKPPEGLREFLTQWLEACR"
     gene            complement(4971..7259)
                     /locus_tag="VFC15_16290"
     CDS             complement(4971..7259)
                     /locus_tag="VFC15_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZM11767.1"
                     /translation="MRLFSIRLIVSLIVGITLVSLASSYYETLGEKRSLRRDLERRAE
                     VLGESLAGNVERDLEKDSTQDLQRIVEHFGNREHLSGLAIYGPQGNLLAVTPELTSTM
                     STVPSVISQALRENHGTGVFQRVGSASVHIYALPLHQHDEVIGGLAIVHDAGYIRAES
                     LRIWRDTFLRALAHVFLIVLITLLIVRWSIAGPIARTAQWMRALRTGRVTSRQSANDL
                     DLFRPLVREVAAFAESLKQARSAAEMEAHLRESGESLWTADRLAVHVRKRLENGRLFV
                     VSNREPYMHTRNGKSVEVVVPPSGVVTAIEPILRVCDGTWIAHGSGDADLETVDEHDR
                     LRVPPEDPRYSLRRVWLNKEEEEGYYYGFANEGLWPLCHIAHTRPIFRAEDWKYYQDV
                     NLKFADALLEEMKDVEKPIVLVQDYHFALLPRMIKEKRPEARVAIFWHIPWPAPQAFD
                     ISPWRRELIDGLLGADLIGFHIQSHCNNFLQTVDRVLEARVDWEHFSVRRLDHLTLVQ
                     PFPISVDFTDVRPPESTPVSYVERSSLLKALGVEATFMGVGVDRVDYTKGILERFLAI
                     ECFLEKYPRYQGQFTFVQIGAPSRSHLKRYHDLMADVEAESDRINGRFQTDTWKPIVF
                     LNRQHNHREIQSYYRAADLCLVTSLHDGMNLVAKEFVAARHDESGVLILSCFTGAAHE
                     LRDALQINPYDIDQTAEAIRCALEMAPEEKKVRMQRMRKTVREHNVYRWAGSLIAELC
                     ELRLDSSDTNTEKLRTSTAAWG"
     gene            complement(7401..8105)
                     /locus_tag="VFC15_16295"
     CDS             complement(7401..8105)
                     /locus_tag="VFC15_16295"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11768.1"
                     /translation="MREMIISELSQALDELARGFAHFLPRLIVMLILMSVGWVIAYVL
                     RVTLRSVLRLIKFDKLSDKAGASQLLTKAALPSATELLSQFVFWVAWLGFILLGVSVL
                     GIMGLQEQIARFFLFLPRLFVAIFILFFGMLAASFFSRAALLAAVNANVPSPRVLNLA
                     VRTIIIIFVLSIVFEELGLAQHTMLIAFGIVFGALMLGLAIAFGIGGRDLAQHFLEKR
                     FVREKKEENEDELSPL"
     gene            complement(8113..9387)
                     /locus_tag="VFC15_16300"
     CDS             complement(8113..9387)
                     /locus_tag="VFC15_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11769.1"
                     /translation="MAEESFLSDDFLRQLINVGEVDILVGLPTHNNAKTIGSVVHAIQ
                     SGILRCFPRERAVIIDADGGSRDGTPELVTGISIDDVRQTSNLYALRTLHSISTTYAA
                     TPSSGMALRAILAAADLLRAKACVVMSPGSANVDSEWLASLLRPVYRDGFELVTPTYR
                     RHKFEGMLITNLLYPMIRALYGVRMREAYPPEFGFSGRLGSQFLGQHVWSDAASGDDD
                     SVELRFILTAITGGSRICQSFLGDKDHIERRAADLVPALRQTAGALFSALETDFSVWS
                     SVAGSNPIPTTGADQAVLLEPMRINRKRLREMFATGVAELESVFQSILSPSTLAELQQ
                     IARLGEEEFRYPAELWVKTVYEFAASYQKSVISRDHIIQALAPLFRGRVLTFVIENRN
                     ASADDVENNIEGLCLEFERLKPYLLQIWKSRE"
     gene            complement(9471..11111)
                     /gene="treS"
                     /locus_tag="VFC15_16305"
     CDS             complement(9471..11111)
                     /gene="treS"
                     /locus_tag="VFC15_16305"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZM11770.1"
                     /translation="MQLNRNPLWYKDAIIYQVHVRAFCDGNGDGIGDFQGLEEKLDYL
                     QQLGISAIWLMPFFPSPLRDDGYDISDYNSVHSSYGTLEDFRKFLASAHDRGIRVIIE
                     MVLNHTSDQHPWFRESRSSRDNPRRDWYVWSDTNTRYKEARIIFLDTETSNWEWDPIS
                     KSYYWHRFFSHQPDLNYDNPAVREQMWKVMEFWLEMGVDAFRLDAVPYLVEREGTNCE
                     NLPETHAVLKELRRRLDEQFPGRMLLAEANQWPADLRPYFGDGDEFHMAFHFPLMPRM
                     FMGVKLEDRKPITEILQQTPEIPPSCQWSLFLRNHDELTLEMVTDMERDYMYDTYAGN
                     KTMRLNLGIRRRLAPLLDNDRRRIELMNGMLMSLPGTPIIYYGDEIGMGDNISLGDRN
                     GVRTPMQWNGGWNGGFSTADPGRLYAPLIDDPVYGYPAVNVLRQTESEHLLLNWMRRI
                     IAVRRSTAVFGRGSIEFLYPASHRILAYVRQLGNEAILVVNNLSSTAQAVELDLRRYQ
                     GYILIEMSGKNLFPRVGELPYLLTLGPYQFYWFRLRRI"
BASE COUNT         2530 a         3371 c         3225 g         2382 t
ORIGIN      
        1 tgacgtgacc ggcgaatttt gtaccaggga aaatgagaga gaatctggta cgagaggagc
       61 tggaagatgg cccgaaagaa acacagtcct gaacagatcg tcgcgcttct gcgacagatc
      121 gaagtggcaa tcgcaagcgg aaaactcacc ccacaggcat gccgcgaagc cggcatcaaa
      181 gaacagagct actaccgctg gcgcaaggag tatggcggtc tgaagttgga gcaggcccga
      241 cggctgaaag agctggagaa agagaatagt cgtctgcgga gactggtgac cgagttgtcg
      301 ctggagaagc aagtcttgaa ggacgtggcc tcgggaaact tctaagccct gaacggcgac
      361 gttgcgcggt agagcatgct catcagcagt atgggttttc cgagcggcat gcctgccggc
      421 tgttggggca gtggagaggg acgcagcggt atcttccgac gcagcaccac gacgaggatg
      481 gcctcacccg ggccatcctg cagttggcca gccaatacgg acgctacggg tatcggcgga
      541 tcacggtgga gttgggcaag gcgggttggt gtgtgggcaa ggaccgggtg cagcggatct
      601 ggaggcggga gggtctgcga gtgccgcaga aacagcgccc gcggggacgc ttgtggctga
      661 acgacggctc gtgtgtgcgg ctgcggccgg agcgggagaa tcacgtgtgg agttacgact
      721 tcgtcagcgc cagcacgcac gatggacgaa ccctgcggct gctgacgctg atcgacgagt
      781 acagccggaa gtgtctggcg atccgggtag cgcggcggtt gaacagttac gatttgatcg
      841 agaccctggc cgatgtgatg ctggtgaacg gtgtcccgga gcacatccgt tccgacaacg
      901 gcccggagtt tgtagccaag cagatgcggc agtggctagc cggggtgggg tcaaagactt
      961 tgtacatcga acccgggagt ccgtgggaga acgggtactg cgagagcttc aacggcaagc
     1021 tgcgggatga atgcctgaac ggagaaatct tctactcgtt gaaggaggcc caggtggtga
     1081 ttgagcaatg gagggtgcac tacaacacgc aacggccgca ctcgtcgctg ggctacaggc
     1141 ccccggcgcc ggtggcaatc ttggccaagc aaccggggca tggggatgtg gaaaacgcta
     1201 cgcgtttccc acatccccac acccccgact acgactacgg acaaatatcc aacgaggcgt
     1261 tacactaaca ttccacttgg tacaaaacat cggtcaggtc ataatcacaa cctataatca
     1321 acatgttcgg ctccttcctc ccgagccttg gttgtttggc agctatcaag tctactcaac
     1381 tcgagggagc cgacattgtt atgaaatcaa gtggcggtgc caatgcggtt gccggatgct
     1441 ggccgacttg aacgaagccg ctgcggcggc ggacgcttac gcgtgtttct gaaccgacgt
     1501 tgtgagagct gggctaactt gggacgcaga gaaggaggaa ctctctgtga cccatctacg
     1561 tcagctcatg cttgaggagt tgcggcgccg caacttcgcc gatacaacca tccgtacgta
     1621 cttgcatggt gtcgaacact tcagtcgata cttcgatcgc cagcccgata agctcggccc
     1681 cgagcatatc cgccagtatc aggcagctct gttcaccaag tttaagttca gctccaacac
     1741 tgttggcttg cgcttggcca cgctgcgctt cttttacatc caggtcttga agaggaactg
     1801 gagcatcgct gagacgccct atccgaagaa ggtgtttcac cttcctcaga tactgagcca
     1861 ggaagaggtc gcccgactga ttgacgccgc tgagcaccca tttcaccgca ttttgctcat
     1921 gacgctctat gccacaggcg cacgtcgcgc agaagcagcg catctgaagg tcagcgatat
     1981 cgacagtaag cggatggtcg ttcatatccg cgaaggcaag ggcggcaagg accgcgacgt
     2041 catgctcagc ccgaagttgc tcgaggaact gcgcacgtat tggcgcggtc tccggcgcaa
     2101 gccaaccgac tggctgtttc ctggcaaccg atggcatacg gggagtcatc ctgtcacaac
     2161 caaggtactc tggacggcct gccagaacgc cgcccaacga gccggccttg cccacaagca
     2221 tatccacccg cacactctgc gccactgctt tgccacacac ttgctcgagg ccggcgccga
     2281 cctgcgcacc atccagatgc tgctcggaca tcgagacctt gaagagacga cgatctatct
     2341 gcatctctcc caacggcatc ttagtgcaac tgccagtccg ctcgatgcac tcaccttcac
     2401 ggcacgagga gaacagaagc acagttcatg agccggcctc ccctagaggt ggccgatatc
     2461 gttcgctgtg caggacagtc ctttcttgaa aacagtcgca ggtggatgaa ctggcaacac
     2521 cagaaggtgc tgctggccat cacgcgctgc cgcaccgccg cgctaggcgg acatcgcgat
     2581 cgctgcacgg gatgcggacg taccactgcc atctcttaca actcgtgcag aaaccggcat
     2641 tgtccaagat gccaaggcaa cgcgcgtctg cgctggctcc aggcgcgcga acgagaactt
     2701 ctgcccaccc actatgggca tgccgtcttc acgctgccgc gcgaactggc cccactcgcc
     2761 ttgcaaaaca agaaggtgat ctatgacctg ctgtttcatg ccagtgctga gactttgctc
     2821 gagatcgccc acgatccccg gcatctgggc gccgagattg gcttcttcag cgtgctgcac
     2881 acctggaatc agcgactgca acatcatcct cacgtccact gcgtgctccc cgccggcggt
     2941 ctggctccgg atcactctcg ctggatctcc tcacgcagat ccttctttct tcccatcaag
     3001 gtactcagcc gcgtcttccg cggcaagttc gtcgccggac tccgcagcgc tttccttaca
     3061 ggcacgctcc agttccacgg cagacttctg cccctggccg agccgcgtgc cttcgcagca
     3121 tggctgcgcg tactcttccg ccatgactgg gtcgtctact ccaagcgccc cttcggagga
     3181 ccggaaaatg tgctgcgcta tctcggcgcc tacacccatc gcgtggctat ctccaaccgc
     3241 aggctggtcg cgctcgccga gaaggacgtg accttccgct ggcgagattc cgcccatggc
     3301 aacaagaaga ggtccatgac gctgcccgtc gaggagttcc tgcgccgctt cctgttgcac
     3361 gtgcttccca aaggcttcgt gcgcatccgc aacttcggct tcctcgccaa caggcaacgc
     3421 gctaccctcc tgccgctctg ctttcaactg ctcggtgact ccgagagcag acccacttcg
     3481 gcaccactgc cctctaccga ccagccgcgc tcactctgga actgtcccgc ctgcggtgga
     3541 accatgcacg tcgtagaacg gctctctgcc gctcaactct tgctccgctc tccacctgaa
     3601 ctcgaacagt gtgcagcatg accttcgatc cacacccgcg gcctctgctc gtgctccagc
     3661 acgcacggag atcccctgtc ttatcgggct tgggaggctt gctagttgat cctttcaacc
     3721 gctgccctac acctgcacgc gctgctattc cgcctactcc ggcccagcca acagggaccc
     3781 caatccaaaa cagcaggttc gtagactcct ggccgccgat cagttccatt caagtcccat
     3841 aggcttccat cacccggggc ggcttccttc aagtcgctgt atctgaagcg ccctgtctaa
     3901 gatgcaagtg cggactgttg cgtgatgggc gcttcagata cagccctaag acttcccagg
     3961 actaatctcc tgatggcggg actggactta cttccgtatt gttgaccatc ctggaatggt
     4021 tcgttggcag ttcgtggaac ttgatcgaga gatcattgcc cacaactgcg tgagtttcta
     4081 agtcgattgc aagcaacaat cgcgaacatc ccgaaagaat cctgcgtgtc atcattcgaa
     4141 gccagaacac tctctctcag cgagatctta gctctaacgg caagcttcca gccactgtgt
     4201 caggaactcg cgtaagcctt ctggcggttt aagccatagg gccgccgctg ttgtgcgcca
     4261 ttcggggcga accagaacgc ttaggcctcg gattcctagc gcgaggaaag cccgctcgtc
     4321 ggtcgcgtca tcacccaggt aggcaacggg atcctccgga ccgatttcat ccaggagggt
     4381 gcggactgca tcccctttgt cacgaccggg catgcgcatt tcaatgccga tgtcaaactc
     4441 cagcagttcc attggtgtat tttgagcaat cgggagccag cccagcaaaa cctggttgcg
     4501 ggcttccgcg gccgtcactt cgtcgagccc tcgtaaatgg atggcaacgg caccggtctt
     4561 gaattcagcg tggtggtgca acccctgata ccttaaccag cgatctgcgt cagccagtgc
     4621 gtgccgcacg ggctcgtcca cgctcggtgt ctcataggtg ccatccggtc tcaaccgctc
     4681 caacccatgg catccccaga tctcgagaga gggatgaacc gtaagtagcg ggaccacttc
     4741 gcgggcgttc cggcccgtga taatgaccac acgggtgcgg ccattgtcga tgatttgctg
     4801 gaggagcgcg gtcacttccg gataggggag agcctcttgg cgattgaggc agaatggcgc
     4861 gagagtcccg tcataatcga gcaacagcgc agaagccggc gactgtgcta ccgcgttcat
     4921 gaattgctcg actacgatgg ggttggcggt gcgggacaac cacatgctcc tcatccccac
     4981 gcagcagtac tcgtccggag cttctccgtg tttgtgtccg acgagtccag gcggagttca
     5041 cagagttccg caatgagact tcccgcccag cggtaaacgt tgtgctccct taccgtcttc
     5101 cgcatgcgct gcatacgaac tttcttttcc tccggagcca tctcaagcgc gcaacgaatc
     5161 gcttcagccg tctggtcgat atcgtacggg ttaatctgca aggcatcgcg cagctcgtgg
     5221 gcggcgccgg taaaacaact caggattaaa acaccgcttt cgtcgtgccg ggctgccacg
     5281 aattccttgg ccaccaggtt catgccatcg tgcagcgacg ttaccaggca caagtccgca
     5341 gcgcggtagt agctttgaat ttcccggtgg ttatgctgtc ggttcagaaa gactatgggc
     5401 ttccatgtgt ccgtctggaa tcgcccgttg atgcggtcgg attccgcttc cacatccgcc
     5461 atgagatcgt gatagcgctt gaggtgactt cgactgggag cgccaatttg cacgaatgtg
     5521 aactggccct ggtagcgagg gtacttctcc agaaaacact caatggccag aaaacgctcc
     5581 agaatacctt ttgtgtaatc gacccggtct acgcccacac ccataaaggt agcctcaact
     5641 cccaaggctt tgagcaaaga agaacgttcg acataagaaa ccggtgtcga ctccggcggg
     5701 cgcacatctg tgaagtccac actgatgggg aaaggttgca ccagggtgag atggtccaga
     5761 cgtcttaccg aaaagtgttc ccaatcgaca cgtgcttcca gtacccggtc cactgtctgc
     5821 aagaagttgt tgcaatgtga ctggatgtga aagcctatca gatcggcgcc gagcagaccg
     5881 tctatcaatt cgcgcctcca tggagagata tcaaatgcct gcggggcggg ccacgggatg
     5941 tgccagaaga tcgctaccct ggcctccggc cttttttcct taatcatccg cggcaacagc
     6001 gcgaagtggt aatcctgcac tagcacaatg ggcttctcca cgtctttcat ttcttcgagc
     6061 agcgcgtcgg cgaacttcag gtttacatct tgatagtatt tccaatcctc cgcgcgaaag
     6121 atggggcggg tgtgcgcaat gtgacagaga ggccagaggc cctcgttggc aaaaccgtag
     6181 taatatcctt cttcttcctc cttgttcagc caaacccggc gcaagctgta gcgaggatct
     6241 tccggaggca cgcgaagccg gtcgtgttca tcaaccgttt cgaggtcggc atcgccactg
     6301 ccgtgggcaa tccaagttcc gtcacagaca cgcagtatcg gctcgatcgc ggtgaccacc
     6361 ccgctgggtg gcacaacgac ttccactgac ttcccgttgc gcgtgtgcat gtaaggctcg
     6421 cgattcgaca cgacaaaaag gcgcccgttc tccagtcgtt tgcgaacgtg cacggccaac
     6481 cgatcggccg tccacagaga ttcacccgac tcccgcaggt gggcttccat ctcggccgcc
     6541 gaccgagcct gcttaaggct ttcggcgaag gcggcgacct cccgcaccag gggacgaaag
     6601 aggtccaggt cgttggcgct ttggcgtgaa gtgactcgcc cggtccgcaa agcgcgcatc
     6661 cactgcgccg tacgcgcgat gggtccggca atactccagc gaacaatcaa tagcgtgatc
     6721 agcacgatga gaaacacatg tgccagcgct cgcaggaagg tatcgcgcca aatgcgaagg
     6781 ctttccgccc gaatatagcc ggcatcatga acgatcgcca ggccgccgat gacttcgtcg
     6841 tgctggtgaa gaggtagagc gtagatgtgc accgaggcag agcccactcg ttgaaacaca
     6901 cctgttccat ggttctcgcg caatgcttga gagattacgg aggggaccgt agacatcgtc
     6961 gatgtcaact ccggggtcac cgccagcaaa ttgccttgcg ggccgtagat agccagtccc
     7021 gagagatgct cgcggtttcc gaagtgctcg acgatgcgct gcagatcctg cgtcgaatct
     7081 ttctccaggt cacgttccac attgcccgcg aggctttcgc ccaacacttc ggcgcgtcgc
     7141 tccaggtccc gacgcaagct acgcttctcg cccagcgtct cgtagtacga ggacgcgagc
     7201 gagaccagcg tgatgcccac gatgagtgaa acgataagcc ggatgctgaa tagtcgcatc
     7261 gcagaaacct ccaaagagcg cagggaggat attgcatcct accgcaacgg gcggcgagtt
     7321 gacattatgc aagggagcct gcgaggacgg gatccggcga gttgtgaccc ggaccgcgtc
     7381 tttccgagct tgtcgctgcg ctacagcggc gacagttcat cttcgttctc ctccttcttc
     7441 tcgcgaacaa acctcttctc aaggaaatgt tgggccaggt ctctgccgcc aatcccgaat
     7501 gcgattgcca agccgagcat tagggccccg aagacaattc cgaatgcaat cagcatggtg
     7561 tgctgtgcca ggcctagttc ttcaaagaca atggacaaca cgaaaataat aatgatggtg
     7621 cggacagcaa ggttcaagac ccggggtgaa ggcacattcg cgtttacggc ggccaataga
     7681 gcggcgcgag aaaagaagct cgctgccagc attccgaaga acagaatgaa tatcgctacg
     7741 aaaaggcgtg gcaggaaaag aaagaacctt gcgatctgct cctgcaggcc catgatcccg
     7801 agcacactca cccccagcag aatgaagcct agccatgcca cccagaaaac aaactggctc
     7861 aagagttccg tcgccgaagg cagagcggcc ttggtgagga gttgtgaagc gcctgccttg
     7921 tccgaaagtt tgtcgaactt gatgagccgt agaacgctgc gtagtgtcac tcgtaagaca
     7981 taggcgatca cccagcccac ggacatgagg atcagcatga cgatgagtcg cggtaggaag
     8041 tgtgcgaaac ctctggcaag ctcgtccagc gcctgactta gttcgctgat gatcatttcc
     8101 cgcatagttg cctcactctc tcgatttcca tatctgcagc agatacggtt tcaatcgctc
     8161 gaactcaagg cacaagcctt cgatattgtt ctcgacatcg tccgcagaag cgttgcggtt
     8221 ctcgatcacg aacgtgagca ctctcccgcg gaaaagcggc gccaaggcct ggatgatgtg
     8281 atctcgactg atcacggact tttggtacga ggcggcaaac tcataaactg ttttgaccca
     8341 caattctgcg gggtaccgaa actcctcctc tcccaggcgg gcgatttgtt gaagttccgc
     8401 tagggtagaa ggtgaaagaa ttgactgaaa gacggactcc aactcggcaa ctccggtcgc
     8461 gaacatctct cgcagtcgct tgcgattgat ccgcatcggc tctagaagca ccgcttgatc
     8521 agctccagtt gtggggatcg gattggaacc agccacgctg ctccagactg aaaagtcggt
     8581 ctctaaagcg gagaacaggg cgccggcagt ctgtcgcaat gcagggacca agtcagcagc
     8641 ccgtcgctcg atgtggtcct tgtcgcccag gaaagactgg cagatgcggc ttcctccggt
     8701 gatagcggtc aggatgaagc ggagttccac gctatcgtca tcaccgctag ccgcatcact
     8761 ccagacatgc tgtccgagga attgacttcc gagacggccg gaaaatccaa actcgggcgg
     8821 gtaagcctca cgcatcctta ccccataaag agcgcggatc atcgggtaca gcaaattggt
     8881 tatgagcatc ccctcaaact tgtgtctgcg ataagtgggg gttaccagtt cgaagccgtc
     8941 acgataaacg ggtcgtaaga ggcttgcgag ccattcggaa tcaacgttgg cggacccggg
     9001 tgacatcacg acacaggcct tggcgcggag caaatcggcc gcagcgagga tggcccgcag
     9061 ggccattccg ctcgaaggag tggctgcata agtcgtactt atcgagtgca gcgtgcggag
     9121 cgcgtataga ttggaggtct ggcgcacgtc atcgatcgag attccggtga cgagttcggg
     9181 agtgccatcg cgtgatcctc catctgcatc aatgattacg gcccgctccc ggggaaagca
     9241 gcgcaggatg ccactctgaa ttgcatggac caccgagccg atagttttgg cgttattgtg
     9301 agtgggtaag cccaccagga tgtcgacttc gccgacgttt atcagttggc gaagaaaatc
     9361 atccgagaga aagctttctt ctgccaattc catctccgtc cgaaatgctg cggcgtgcca
     9421 ttgagtagac gcacgtcaag tcgcatcggt ttcaacgtct cgttacttgc tcagattcgc
     9481 cgtagccgaa accagtaaaa ctggtacgga cccagcgtta acagatacgg cagttcgccc
     9541 acgcgcggaa acaggttctt gcccgacatc tcgatcagga tatatccctg atatcgtcgc
     9601 agatccaact caaccgcttg cgcggtgctg gaaagattgt tgaccacaag gattgcctcg
     9661 tttcccagtt gccgcacata ggccagtatt ctgtggctgg ctggatagag aaactcaatc
     9721 gatcccctgc cgaacacagc ggttgatcta cggaccgcta tgatgcgccg catccaattc
     9781 aggagcaagt gctcgctctc cgtttgcctt aagacgttta cggctggata accgtatacc
     9841 ggatcgtcga taaggggggc atatagacga ccgggatccg cggtcgaaaa gccgccgttc
     9901 caacccccgt tccattgcat aggagtccgc actccgttgc ggtcacccaa actgatgtta
     9961 tcgcccatcc cgatttcatc gccgtaatag atgatcggag tgccgggtag cgacatcaac
    10021 atcccgttca ttagctctat gcgccttcgg tcattgtcca acaaaggagc gagccgcctc
    10081 cgaattccaa gattcagccg cattgttttg tttccagcgt aggtgtcgta catgtaatcg
    10141 cgttccatgt cggttaccat ctccagggtc aactcgtcat ggttacgcag gaacagggac
    10201 cactggcagg agggcgggat ttccggggtc tgctgcaaga tttcggtgat gggcttccgg
    10261 tcttccaact tcacgcccat gaacatcctg ggcatcaaag gaaaatgaaa cgccatgtgg
    10321 aattcatcgc catcaccgaa gtacggccga aggtcggcgg gccactgatt ggcctcggcc
    10381 agaagcatcc tgccggggaa ctgctcgtcg agccttcgcc gcaactcctt cagaacagca
    10441 tgggtctccg gcaagttctc gcagtttgtg ccctcgcgtt ccactaggta cggcaccgcg
    10501 tctagccgga aggcgtcaac acccatctcc agccagaact ccatcacctt ccacatctgt
    10561 tctcgaaccg caggattgtc gtagttgaga tcgggctggt gactgaagaa gcgatgccaa
    10621 taataggatt tcgaaatcgg gtcccactcc cagttggatg tttccgtgtc gagaaatatg
    10681 atgcgggctt ccttgtaccg ggtgttggta tcgctccaga cgtaccaatc gcgccgcgga
    10741 ttgtcccgcg aacttcgcga ctctcgaaac cacggatgct ggtctgatgt gtggttcaac
    10801 accatttcaa tgatgacgcg gatcccacgg tcgtgggccg acgctagaaa cttgcgaaaa
    10861 tcctctaatg ttccgtagct ggaatgcacc gagttgtaat ccgagatgtc gtaaccgtcg
    10921 tcgcgcaggg gagaagggaa gaatggcatc agccagatcg cgctgattcc cagttgctgc
    10981 aggtaatcga gcttctcttc aagtccctga aaatcaccga ttccatcacc gttgccgtcg
    11041 cagaaagcgc ggacgtggac ttggtagatg atcgcgtctt tgtaccaaag cggattccgg
    11101 ttgagttgca agacgcttcc tcctccccca atatcagttc ccctttgcaa agcagagtgc
    11161 cacgcaagtt tatatgaagt gagaaacgaa gaaaggagta ttgcaagcct ccaacccaga
    11221 ctgggctttg catgtgtata tgtgcgtaac gggcaaacct tctgttatcg gcgcatgcgg
    11281 tttagcgtgc gactcaccag ccgttgaaga gcgaacccac gttaggaact ggcgccgaag
    11341 attgcccaga agtcttcttg tgggaagccc tctgtgtcaa caatagtgag acatctacgc
    11401 catcaacaat tactgtaggg gcggagaaag aattctcacc ttcatgagca agactagcgt
    11461 tcaacgaatg gagtgcaatg ctgcggagtt gggagctccg gagcggag
//
LOCUS       DASRKK010000669         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1238075, whole genome shotgun sequence.
ACCESSION   DASRKK010000669 DASRKK010000000
VERSION     DASRKK010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238075"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFC15_16310"
     CDS             complement(<1..396)
                     /locus_tag="VFC15_16310"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZM11771.1"
                     /translation="MSISIKSVFLVCIVAGSFVAGAWYNRRPEVARAAPGTRRVLYYH
                     DPMHTAYRSDKPGIAPDCGMQLEAVYAGDSAVLGQGQQVSAVSVEISPEKRRSIGLVT
                     DTIERASAQHTIHVLGRVTLDETRLVRVTV"
     gene            complement(514..2109)
                     /locus_tag="VFC15_16315"
     CDS             complement(514..2109)
                     /locus_tag="VFC15_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11772.1"
                     /translation="MATIPELLDGHVTLEVECLDRLYLNGYIGQLATGWGLVGFMRGQ
                     LEKPVPSPVVLGQVSERFREAVKAQAERDQIPIYQFQHKERKDDIANDFRRQRQVRDE
                     IVFIGVAQEKAQAFNGKKVNGQFEFNRDKTVYVNHYYFYIDDEDFGPLFLKVCSYAPW
                     STKLCLNGHEWAKRQLEKKGIAYEALDNGFLSCAAPEQLQQICDSLGPQDIDRVFRKW
                     LKRIPLPLRPQDREAGYDWDLSIWQMEVSLTQIFDRPLRGREFFEEIIRDNLDLGRPD
                     RIQLVFERAVTKKTPGQFRTRVIQKGVHPSLHIEYKNFDLKQYFKEGRGCRTEGTFRN
                     PKDFGINKGLSNLPYLQQIGRQINRRLLEVERVSHNSGLSGDSIQRVVQPAVTEDGKK
                     APGLKFGQPRVMALLLALTMFHHLIDGFRNHDLRQQVADLLGVTLSEYTPGQMTYDLR
                     RLRLKGLIYRPPKTNRYFVTPYGWKVARLFSRLDARVFRPAMAMFTANDAVLPFPLRQ
                     SLDRVDADLDLLIYDAFPLLKTG"
BASE COUNT          496 a          702 c          658 g          527 t
ORIGIN      
        1 cacggtcacg cgcaccagcc tcgtctcatc cagagttacc cgtcccaaca cgtgaatagt
       61 gtgttgggcg gatgcccgct caatagtatc ggtgacaagc ccgatactcc gtcgtttttc
      121 gggactgatc tccaccgata ccgccgatac ctgctggccc tgtccgagta cagcagaatc
      181 gcctgcataa accgcttcga gttgcatgcc gcaatccgga gcaattccag gcttatcgga
      241 gcgataggcc gtgtgcatcg ggtcatgata gtagagaacg cgacgggtac cgggagccgc
      301 ccgcgcaacc tccggcctgc ggttgtacca ggcgccagcc acaaaagagc cggcgacgat
      361 acagaccaga aagactgatt taatcgagat agacatggga acgccaaaac cgacagacgg
      421 cagggagtcc gcactccctg ccgatcagtc cgccaggagc agcggactct agccgtctaa
      481 tgtaagtgag ttctttctga aagtgtcaag ttttcagccc gtcttcaaca ggggaaaagc
      541 gtcatagatc agcaggtcca agtcggcatc gacgcggtcc agagattgac gaagcgggaa
      601 tgggaggacg gcgtcgttgg cggtgaacat cgccatggca gggcggaaga ctcgtgcatc
      661 gagtcgggag aacagccgcg ctaccttcca tccgtacggt gtgacgaaat agcggttggt
      721 tttcggcgga cggtagatca gccctttcag ccgaagccgt cgcaggtcgt acgtcatctg
      781 gccgggggtg tactcgctca gggttacccc cagcagatcg gcgacttgtt gtctgaggtc
      841 gtggttgcga aagccgtcga tcaagtgatg aaacatggtg agcgccagca ggagcgccat
      901 cacccttggt tggccgaatt tcaatccggg cgcctttttg ccgtcttcgg tcacggcggg
      961 ttgcaccact cgttggatgc tgtcgccgct caagccgctg ttgtggctga cgcgttcgac
     1021 ttccagcaag cgccggttga tctgtcggcc gatctgctga aggtatggca gattgctcag
     1081 tcccttattt atgccaaagt ccttcggatt gcggaatgtc ccctccgtgc ggcagccgcg
     1141 accttccttg aaatactgct tcagatcgaa gttcttgtat tcgatatgca ggctggggtg
     1201 aacacctttc tggatcacgc gtgtacggaa ctgccccggc gttttcttcg tcaccgctcg
     1261 ctcaaagacc agctgtatgc ggtccggccg gcccagatcc agattgtcgc gaatgatctc
     1321 ctcgaagaat tcgcggccac gcagtggacg gtcaaagatc tgcgtcaggc tgacctccat
     1381 ctgccaaatc gacagatccc agtcatagcc cgcttcccgg tcctgcggac gcagcggcag
     1441 tggaatccgc ttcagccatt tgcggaacac ccggtcgatg tcttgcggcc ccaacgaatc
     1501 gcagatctgc tgcagttgct cgggcgcggc gcaggacagg aacccgttat ccagtgcctc
     1561 gtaggcgatt ccctttttct ccagttgccg cttggcccac tcgtgtccgt tcaggcagag
     1621 ctttgtgctc catggagcgt agctgcacac tttcagaaac agcggcccga aatcctcatc
     1681 gtcgatgtag aagtagtagt ggttgacgta aaccgtcttg tcgcggttga actcgaactg
     1741 cccgttcacc tttttcccat tgaacgcctg ggctttctct tgcgctaccc cgatgaagac
     1801 gatctcatcc cgcacctgtc gctgccggcg aaagtcattg gcgatgtcgt ctttccgctc
     1861 cttgtgctgg aactggtaaa tcgggatctg gtcgcgctcg gcttgtgcct tcacagcctc
     1921 gcggaaccgt tccgaaactt gcccgaggac tacgggcgag ggaaccggct tctccaactg
     1981 gccccgcatg aagcctacca gcccccaccc cgtagccaac tgtccaatgt agccgttcag
     2041 gtaaagacga tcgaggcact ccacttccag cgtgacgtgc ccgtccaaca actccgggat
     2101 agtcgccatc ctcggatttt acgaaatact ccacccgtgt gctcgtcggt ttgaggtcga
     2161 agacctcgct agctattgtc atagcatagg caggaagagt ctcaggggta cgaatggcac
     2221 ccttctttat ggaactaata agatcaagaa tggtgtcatg gtacgtatcc tgggcgcgat
     2281 cccacccaat ttgtctacta aggaagaagc gtaaatactt taataactca tacatctcag
     2341 ccacagctgg ttcactccca gcctcaatct gctccagtat ggg
//
LOCUS       DASRKK010000670         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_847285, whole genome shotgun sequence.
ACCESSION   DASRKK010000670 DASRKK010000000
VERSION     DASRKK010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847285"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..820
                     /locus_tag="VFC15_16320"
     CDS             470..820
                     /locus_tag="VFC15_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11773.1"
                     /translation="MATWVSGISDSGDIVGYYNDGTTFHGFLLSKGKFTGIQPPASDG
                     DLARGINARGDIVGQFGQADVPHCFLYRKGTYIIVDPPGSTLSFGRAINARGDIVGYY
                     ADSAGANHGFAATR"
     gene            1289..1732
                     /locus_tag="VFC15_16325"
     CDS             1289..1732
                     /locus_tag="VFC15_16325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZM11774.1"
                     /translation="MPKPEYPTTAFCFKVAFAGSTEVDQAFQEVRGIGAELETEEVAE
                     GGENRFTHRLPKTIKHPLLELKRGIAPQTSALVGWCKSVLEFGKAIETKAIVVFLLDP
                     DGKPLLGWQFSDAYPVKWEISDFNSMKNEVTIETISLSYTFSKRL"
     gene            complement(1708..2265)
                     /locus_tag="VFC15_16330"
     CDS             complement(1708..2265)
                     /locus_tag="VFC15_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11775.1"
                     /translation="MSHASWPCPRNTNYAAAVKPTAQLRSYWNQTARQLGHRSGNWLS
                     LEQSSQLVNCASGDGLRERRDCAMLAILVGCGLRRAELANLPVEKIQVRQGHWVIVDL
                     VGKGGHVRTIPIPRWAKEKLDRWTSAATISTGRVFRAIRKNGTVWGDRINVNVVWHVV
                     NRYCRSIGLEHVAPPAVAHSLLENV"
     gene            complement(2168..>2378)
                     /locus_tag="VFC15_16335"
     CDS             complement(2168..>2378)
                     /locus_tag="VFC15_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11776.1"
                     /translation="RYPYSRKGETMSNVEKYETLTRALAAALFRLGVGTAVFEPRELA
                     LPEKYKLRCRRQADGSVAVILEPNS"
BASE COUNT          520 a          660 c          672 g          526 t
ORIGIN      
        1 cagttcgcat gcctttgtca gtgatgtggc gtgaagtaac gaaaatagcg ctcggctcct
       61 cacgattgcc tcccgctgtt atgtcacgcc agagacatgc gctgacagac aagaatcctc
      121 gatctatgga ggcgttgaac agtaactcgc gaaaagtctt acggctgtat cggaagcgcc
      181 cctacgagga atcctggatt tcattggagt gtgcgggcgc tttcgataca gcgacttgaa
      241 ggaagcccct gactatggga tttgattggc gctgggttga gacgtcaccc acggctttgt
      301 tttcaggcaa ggtcggttca caccgatcga ctttcccgga gcgaacgaca cgttcaatgt
      361 caccaccaac gacgccggcg acatcgccgg cagctacttc gacgccagca ccgttcacgg
      421 cttcacgctc atccgaggga agttcacctc cgtcgatttt ccggggagct tggcgacttg
      481 ggtttccggc atcagcgatt caggggatat cgtcggctac tacaacgatg gcaccacatt
      541 tcacggcttc cttttaagca aggggaaatt caccgggatt cagccgccgg cgtcggacgg
      601 agatctggcg cgcggaatca atgctcgtgg tgatatcgtc gggcaatttg ggcaggccga
      661 cgtgcctcat tgtttcctgt atagaaaagg aacttacata attgttgatc ctccgggttc
      721 taccctgtca ttcggccgcg cgatcaacgc ccgcggcgac atcgttggct actacgccga
      781 tagtgccggc gctaaccatg ggtttgcggc aacccggtga agactgagcc agtccctagg
      841 gcttcacctc gggactggct cctcgttgag accccgacat gagtgctttg aggaattgaa
      901 tgattaagct tcctcggagt agaaactcta tccgatcctt caatcggata aaccaccaga
      961 agataggggt tgagccatcg caattacact cattgacagg cgtgatttat acgcaacacg
     1021 gaagcgatcc agaaaccggc atcggggaga ttactccccg caagtcggct agcatccgcc
     1081 gatctttgtt ttgccccact tgggcgtaaa acgcccaacc cgtgctgttg aagaactcca
     1141 ccacgtcgcg gccacaagga cggaacgccg actatacgcc caaatgatcg gcattgcggg
     1201 tggcagaacc acttgttgtg ggataaattg attgagtcca cgtgcgacga ttcgccgaca
     1261 gctaagggcg acctgaagct ttccgcatat gcccaagccc gagtatccga cgaccgcttt
     1321 ttgcttcaag gtggcatttg ctggctccac ggaggtcgac caggcgtttc aggaagtgcg
     1381 gggaattggg gccgaactgg agaccgaaga ggtcgccgaa ggcggcgaga accggttcac
     1441 gcaccggctg ccgaaaacca tcaagcatcc gctactggaa ctgaagcgcg gaatcgcgcc
     1501 gcaaacatcg gctttggtgg ggtggtgcaa gtccgtactc gaatttggga aggcgatcga
     1561 aactaaggcg atcgtggttt ttctgctgga cccggacgga aaaccgttgc taggctggca
     1621 gttctccgac gcttacccgg tgaagtggga gatcagcgat ttcaattcga tgaagaacga
     1681 ggtgacgatt gaaacgattt ccctcagtta cacgttttcc aaaaggctat gagcaacggc
     1741 gggcggggcc acgtgctcta gtcctatgct acggcaatat cgattcacca catgccagac
     1801 aacattcacg ttgatgcgat caccccaaac cgttccattc ttgcgtatgg cgcggaacac
     1861 tcgtccggtg ctgatcgtgg cggcagaggt ccaccggtct aacttctctt tggcccagcg
     1921 cggaatcggt atagtgcgaa catgaccgcc cttgccaacc aggtccacga tgacccaatg
     1981 gccttgacga acctgtatct tctcaacggg caagtttgcc agttcggcac gacgaaggcc
     2041 gcatcccacc aggattgcta gcatcgcgca gtccctcctt tcacgtagcc catcgccaga
     2101 ggcgcagttt accagctggg agctttgctc caagctcagc cagttgcccg agcgatgtcc
     2161 gagttgtcta gctgtttggt tccagtatga ccgcaactga gccgtcggct tgacggcggc
     2221 agcgtagttt gtatttctcg ggcagggcca actcgcgtgg ctcaaagacg gccgttccga
     2281 cgccaaggcg gaaaagtgcg gcggctaagg ctctggtcag cgtttcgtat ttttcgacgt
     2341 tcgacatggt ttctcctttg cgggaatatg ggtatctg
//
LOCUS       DASRKK010000671         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1336309, whole genome shotgun sequence.
ACCESSION   DASRKK010000671 DASRKK010000000
VERSION     DASRKK010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336309"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1837
                     /locus_tag="VFC15_16340"
     CDS             227..1837
                     /locus_tag="VFC15_16340"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM11777.1"
                     /translation="MALPVNDTKRAKLSEPPGHGSGRGQLRQRLRKAERDNERLRQQN
                     EQLHEQNEQLQRQMEESQQELADKDKAIADRDKQIADLERQLAARKRNSTNSSKPPSS
                     DGLAGTQRPRCSLRKKSGRKPGGQPGHMGHDRQPVENPDRVEEVLPQQCKHCGTPLPQ
                     ASGDRQTVGDIFRRQIVDLPEVIVPVVTEYQYPKLVCSCCHKGTRAELRPEHEHEIGE
                     RLTAVISYLIGLRKMTRRDVAATMQDLFGVDISVGSVQKAWEETADAVEAPYTELEEA
                     LATEPVLNSDETGSRTNGDKRWVWALCSSWFVFYHIACSRGVEVLVELLGEAFAGILC
                     SDRCPTYLSYHRGLAQFCWAHLQRTLKGIGEFASTADAVHFARDMLSAVDRLFGLWYQ
                     FRGEAGLGKRLLTRTELIQQSVPIQKKICGLAEKYLDSDDREVRNLACAFYVHWDKLF
                     TFIEHEGVEPTNNVSERAMRLFVLIRKITYGNRSAKGEIALARLLTVTQTCKLQQRPL
                     LGYLLTAVHCHRRRQPAPSLRPLQNQQP"
     gene            1866..>2084
                     /locus_tag="VFC15_16345"
     CDS             1866..>2084
                     /locus_tag="VFC15_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11778.1"
                     /translation="MDTKQSEDSEERPATPRSEAMRKAWDKRRASAATGAEWLAHPEC
                     CCGCSARLAVAKNPEHQSSFKPGHGAALK"
BASE COUNT          432 a          603 c          672 g          377 t
ORIGIN      
        1 gcacctcgga acggctttgt cgtcatcggc cagacggagc gtttattgcg tccgtcgcca
       61 cttggcggaa accctccgct tcggtcctgt tgcggattgc cgactggcgc gccgcttgac
      121 ggctgagcgc cacggccggt gacgtgtgct cgccttgggt aattcgcaac tgttcaggcc
      181 cttattttcc gctggacaaa aactgcaaac catggcaagc tgatccttgg cgctgccggt
      241 caatgacaca aagcgggcga aactttccga accgccgggg catggttcgg gccgggggca
      301 actgcggcaa cggcttcgta aagccgagcg agacaatgag cgactgcgcc agcagaacga
      361 gcaactgcac gagcaaaacg agcagttgca gcggcaaatg gaggagtccc agcaagaact
      421 cgccgacaag gacaaagcca ttgccgacag agataagcag atcgccgatt tggagcgcca
      481 actggcggcg cgcaagcgga actccaccaa ctcctccaag cccccttcgt cggacggctt
      541 agcaggcact caacgccctc gctgctcgct gcggaagaaa agcgggcgca aaccgggcgg
      601 ccagcccgga cacatggggc atgatcggca gccggtggaa aacccggacc gggtcgagga
      661 ggtcttgccg cagcaatgca agcactgcgg aacgccattg ccccaggcat cgggggatcg
      721 tcaaacggtg ggggatatct tccgtcgtca gattgtggac ttgccggaag tgatcgtgcc
      781 ggtggtgacc gaataccagt atccgaaact ggtgtgttcc tgttgccata aggggacgcg
      841 cgccgaacta cggcccgaac acgagcacga gatcggggaa cggctgacgg cggtgatcag
      901 ctacctgatt gggttgcgga agatgacgcg gcgggatgtg gcggccacca tgcaggacct
      961 cttcggcgtg gacatcagcg tgggcagcgt gcagaaggcg tgggaggaga ccgccgatgc
     1021 ggtggaagcg ccctacacgg aattggaaga ggcgctggcc accgagccgg tgttaaacag
     1081 cgacgagacc ggcagccgta ccaacggcga caaacgctgg gtttgggcgt tgtgctcctc
     1141 gtggtttgtg ttctaccaca tcgcttgcag tcggggcgtg gaggtgctgg tggagttgct
     1201 gggggaggca ttcgccggca tcctctgttc ggatcgttgc ccgacctact tgagttacca
     1261 ccgtggcctg gcccagttct gctgggctca cttgcagcgc accctcaagg ggattgggga
     1321 gttcgcttct acggccgacg ccgtgcattt tgcccgcgac atgctttccg ccgtcgatcg
     1381 gctgtttggt ttgtggtatc agttccgcgg cgaggcgggg cttggcaagc gtttgttgac
     1441 gcgaactgaa ctgattcaac aatcggtccc aattcagaag aagatctgtg gtctggcgga
     1501 gaaatacctg gacagtgacg accgcgaggt gcgcaatttg gcgtgcgcgt tctacgtgca
     1561 ttgggataag ctgtttacgt tcatcgaaca cgagggagtg gagccaacca acaatgtcag
     1621 cgagcgagcc atgcgcctgt tcgtgctcat tcgcaagatc acgtacggta accgcagcgc
     1681 caagggtgaa atcgccttgg cccgtttgct caccgtgacg cagacctgca agctgcagca
     1741 gcggccgctt ctcggctatt tactgactgc ggttcactgc catcgccggc gccagccggc
     1801 gcccagcctc cgccctctcc aaaatcaaca accatagggg tctgaacagt tgcgttcaat
     1861 gggatatgga taccaaacaa tctgaagact ccgaagagcg tccggcgaca ccgcgttccg
     1921 aagcgatgcg gaaagcctgg gacaagaggc gagcgagcgc cgccacggga gcagaatggc
     1981 tggcgcaccc cgagtgttgc tgtggctgtt ctgcccgact cgcggtagcc aaaaacccgg
     2041 agcaccagag ttcgttcaag cccggccacg gcgcagccct gaaa
//
LOCUS       DASRKK010000672         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_162898, whole genome shotgun sequence.
ACCESSION   DASRKK010000672 DASRKK010000000
VERSION     DASRKK010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162898"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1497)
                     /locus_tag="VFC15_16350"
     CDS             complement(235..1497)
                     /locus_tag="VFC15_16350"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZM11779.1"
                     /translation="MQRHNNAIQKFFAELGPGLITGAADDDPSGIATYSVTGATFGYG
                     PLWTALFCFPLMAAVQLMCSRLGMATGRGLAAVVRRRYSRWVLWGACLLLIVANVINI
                     AADLGGMAEATHMVTGIPAAWLTPVFAAFIVAALFFTSYRRLARVFKWLTLVLFAYVI
                     TAFIAHPAWWAVLRATFVPDIQWSREYLSVLVGILGTSISPYLFFWGAAQEVEEERAE
                     GKNTVAKRQGATDAELRRARIDILAGMFFSNFVMYFIILTTAATLHAHGITQIATASQ
                     AAEALKPLAGKGAYWLFTLGLIGTGMLGVPVLAGSSAYAIAEAMSWGASLDRKPKLAP
                     KFYGVLGVAMLLGVALNYVGLNAVKMLFWSAVANGVLAPPLIVLVVLLTTDRSVMGDL
                     VNPPLLKLVGWLAAAVMTAAAIAMFLAG"
     gene            complement(1977..2183)
                     /locus_tag="VFC15_16355"
     CDS             complement(1977..2183)
                     /locus_tag="VFC15_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11780.1"
                     /translation="MSDSMYRKWMQAVAKTLQTATEKVNMAIYEIARLPAMLLTKRCT
                     STPSRPYYCIQRKCRSMVLRFHAE"
     gene            2704..>2895
                     /locus_tag="VFC15_16360"
     CDS             2704..>2895
                     /locus_tag="VFC15_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11781.1"
                     /translation="MEEVIHSSSDAHYLTGLSQTSGGNESHSSGVSWAAVIAGAFVTA
                     ALSLILLALGTGFGFSSVSL"
BASE COUNT          605 a          869 c          876 g          545 t
ORIGIN      
        1 attttaggcc tgaatgtatt tctctgcaca ggggctgcca gtcttatggt tcctgttcac
       61 ttgttacggt cttctatact tggggagatc gttttaaagg gggggccccg accgggagtc
      121 cagctagaaa tcccggctcg actcccacag cattcactac gagactcgcg ggctaacgga
      181 cgtagaccga tgccgggaac cgaggtggta ccggacatgg cgaggagtca atttttaccc
      241 cgccaagaac atcgcgatgg cggcggcagt catcaccgcc gcggccagcc atcccaccaa
      301 tttgagcagc ggcgggttca ccaggtcacc catcacggag cggtcggtag tcagcagcac
      361 caccagcacg atcagcggcg gcgcgagcac tccgttggcc actgccgacc agaagagcat
      421 tttcaccgcg ttcagaccca catagttcag cgccacaccg agcagcatgg cgacgcccag
      481 cacgccgtag aatttcggcg cgagcttggg cttacggtcg agcgacgcgc cccaggacat
      541 cgcctcggcg atcgcatatg cgctcgaccc cgccagtacc ggcacaccaa gcatcccggt
      601 accgatcagg ccgagcgtga acagccagta ggcgcccttc ccggcgagcg gcttcaaggc
      661 ctccgccgcc tggctggccg tggcgatttg cgtgatcccg tgagcgtgca gcgtggcggc
      721 ggtggtgagg atgatgaagt acattacgaa gttggaaaag aacataccgg ccagtatgtc
      781 gatgcgcgcg cgccgcaact ccgcgtcggt cgcgccctgg cgcttggcca ctgtgttctt
      841 gccttcggcg cgctcttctt ccacttcctg cgcggccccc cagaagaata gatacggcga
      901 gatgctggtg ccgagaattc ccaccagcac cgaaagatac tcgcgagacc attggatatc
      961 cgggacaaac gtggcgcgca gcaccgccca ccaggccgga tgggcgatga aagcggtgat
     1021 cacgtaagcg aacagcacca gcgtgagcca tttgaaaacc cgtgcgagcc tgcggtagga
     1081 agtaaagaac agggccgcga cgatgaacgc agcgaagacc ggggtaagcc acgcggcggg
     1141 aatgccagtc accatgtgag tggcttccgc cataccgccc aggtcggcgg cgatgttgat
     1201 cacgttggcc acaatcagta gcaggcatgc gccccacagc acccagcgcg agtagcgccg
     1261 gcgcaccacg gcggccaagc cgcggcccgt ggccatgccc agccgcgagc acatcaactg
     1321 caccgccgcc atcaggggga aacagaacag cgcggtccac agcggaccgt agccgaaagt
     1381 agcgccagtc accgagtagg tggcgatgcc ggaggggtcg tcgtcggccg ctccggtaat
     1441 caggccgggg ccgagctccg cgaagaactt ttggatcgcg ttattgtggc gctgcatcca
     1501 ccgcttccac caacaaggcg gccacactag tcccggcccc gagcctacag gatatctcga
     1561 acccgcggcg gttggctaga cctagtcttg tcttatacaa gctccgggac cgacggcagt
     1621 ggctcacaac agaaaggaac actggcagag cctgttgtat gaactttggc aggccgcggg
     1681 tggcgacagt tgggaccgcc gcatcaagtg gagttgttca agcccggtgc cgacgcgcca
     1741 ctgtttaaca gcctgttgga agttggggag cccatcctcg gtggcagaag accttctgaa
     1801 ctatcgaaga aactgcggtt ggcaatgggc cgggacgaca tcggcttaag tgatatgcgg
     1861 agcgccgtcg acggacagcc taaccatcgg cctctacccc gacctgggcg aactggacag
     1921 cgacgagcgc ttcgctgtcc cctggcatga ctatcggccc acaaagcggc atgcatctac
     1981 tcggcgtgga atcgcaaaac catactgcgg cacttccgct ggatgcagta atacggcctc
     2041 gacggcgtgc tggtgcagcg cttcgtgagc agcatcgctg gtaggcgggc tatctcgtag
     2101 atcgccatgt tcactttttc cgtggcggtt tggagagtct ttgcgactgc ctgcatccat
     2161 ttgcgataca tagagtcaga catgccgatc acccggcccg cgactgactg agtgccatga
     2221 acgtcagccc gtcggatatt tgctgcgcca gggtgagcgt gatgttcacg ccattaacgt
     2281 gtagcagaaa agccaccccg cgcccatctc cctgaatccg gcgaggaggg gggattgagg
     2341 tggattccta gaaacgacga ttcctgcgtg aagagcaatt tttgcgcggt aatgtcgccg
     2401 acaaaggcat aacggctttg tacaccgttt cacaaatagg gtctcgtatt ccgaggagat
     2461 cgcccgcggt tcaagtggtg ttgtttcagt tcttcgtctt tggggggccc gcctgctact
     2521 tcccccgagg cccgcgcacc ggttgtactg gtgcccgcag gctaaacaca atcgagcccg
     2581 aaaaaaccaa attcaggtcc tgggattcac cgaaagcgtt tggagtgtca cgtgcctgcg
     2641 atatcagcag agggcataat tgacccacta tttggttgtt ctgaccagaa ggacagaaat
     2701 cgcatggaag aagtaataca ttcatcgtcg gacgcccact atctaaccgg cctcagccag
     2761 acttcaggcg gaaatgagtc acactcgtcg ggtgtgtctt gggcggcagt gatcgctggc
     2821 gcatttgtga cggccgcgtt gtcgcttatc ctgcttgcgc tgggtaccgg gtttggtttt
     2881 tcctcggtat cgctg
//
LOCUS       DASRKK010000673         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_554320, whole genome shotgun sequence.
ACCESSION   DASRKK010000673 DASRKK010000000
VERSION     DASRKK010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554320"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..441
                     /locus_tag="VFC15_16365"
     CDS             85..441
                     /locus_tag="VFC15_16365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HZM11782.1"
                     /translation="MRQCRLWRRLGDHAVTGQSNCVDGAYPQASLVQATDGNFYGTVV
                     GGGQNGIGAVYKVTPSGSLTTLYSFHNSDGSEPFGGLIQARDGNLYGTTNQGGRSGYG
                     TVFRLGAIRPCATCRP"
     gene            905..1990
                     /locus_tag="VFC15_16370"
     CDS             905..1990
                     /locus_tag="VFC15_16370"
                     /inference="COORDINATES: protein motif:HMM:NF025746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4419 domain-containing protein"
                     /protein_id="HZM11783.1"
                     /translation="MAKRFRRKLLLLPDEDRPVTNALYDHTLHLSFAPEKRAQLVGSI
                     VSRCHPLIDAVGTAFSEHRPLTLSPDSIWLVIEQGFVHHVAENAETLRHRLVRHLGTR
                     DLEAQVPDLSLSSFEHAISDWSLQIREATDPVLHETLICEFSTTTPAIRTASEVVLMD
                     CYSSYFTYSMMCICGIPRITVTGSLHDWQRMRARVEVLETFGLEWWVSRLRPILDEFI
                     RTVEGHPSLAFWQAIYKPKQAYGTDAVTGWIADLFPYLSDSPERRRSHVFGFEREDWA
                     VPVEKGVKTEFSLGEPGSEKGVAPKSFPSGLASVPVKLSFPNGLTSGVDLVAGFLAVE
                     QDPEDLAISPVIGWSVTESAPIKPVQI"
BASE COUNT          438 a          654 c          642 g          508 t
ORIGIN      
        1 tgcatttccg ctcggtggcc cagccttgga caagaatggc aatgtgtacg gcacaaccga
       61 gtacggcggc atgcaaggtg gtaaatgcga cagtgtcggc tgtggcgtcg tctgggagat
      121 cacgccgtaa ctgggcagtc gaactgtgtt gacggcgcct acccgcaggc cagcttagtg
      181 caggcgaccg atggaaactt ttacgggacc gtcgttggcg gtggacagaa cggcattggt
      241 gctgtctata aagtcactcc gagcggcagt ttgactacac tctacagctt tcacaactcc
      301 gatggctccg aaccattcgg cggcctcatt caagcgcgcg acggcaacct ctatgggaca
      361 acgaatcaag gcggacgttc tggttatggc acggttttcc gcctgggcgc gattcgcccg
      421 tgcgcaacgt gccgtccgtg agttggagac gagacctgtg attgccgggt gttttgctat
      481 aactggcgca atatcgccat ctcgcacgta atcccggatg agcagcccgt tcggtttagg
      541 tcgcaagttg gcgtgctcac gacagcgtat cgacgcgggc cagaggtccg cttcggggaa
      601 gtcacttgga cgtgtagtcg ccaaaccgct gactgagagc ggacgacacg ccgtaagtca
      661 caagaaaggg tagggcgtga tacggccaag tggcgagtgt cggaatcgca gatgacaagc
      721 cgacttctcg ttactgaatc caagccacca ggggtctttt gtgtcccagg tacaggatct
      781 ggtgtaatat cccgccatgt ctgacacgcc attgcagcaa gagaagagtc ggcccactcc
      841 attcgcagtt gatcctgtcg ttccggcgag caggccacta tggactgaaa ctctgcgcga
      901 ccacgtggcg aaacgattcc gccgcaaact cctgctcctc cctgacgagg acaggccggt
      961 cacgaatgct ttgtacgacc atacgctgca cttgtccttt gcgcccgaaa agcgagccca
     1021 actggttggc tccatcgtca gcaggtgcca tccgctgatt gatgccgtag gcaccgcctt
     1081 cagtgagcat cgtcccttga cgctttctcc tgactccatc tggttagtca tcgaacaggg
     1141 ctttgtgcat cacgtcgccg agaatgccga gactctccgt caccgcctgg ttcgtcacct
     1201 cgggactcgt gatcttgagg cgcaggttcc cgatttaagt ctgtccagct tcgaacacgc
     1261 catttccgac tggtcgctcc agattcgtga ggccaccgat cccgtactcc acgaaacgct
     1321 gatctgcgaa ttttctacca ccaccccggc gattcgcacc gctagtgaag tcgtcctcat
     1381 ggattgctat tcctcctact tcacctattc catgatgtgc atctgtggca ttcccaggat
     1441 cacggtgacg gggtccctgc acgactggca gcgcatgcgc gcgcgggtcg aggtgctgga
     1501 gactttcggg ctcgagtggt gggtttcccg tctacgcccg attctcgatg agttcattcg
     1561 caccgtggaa ggccacccct cgctggcgtt ctggcaggcg atctataagc ctaagcaggc
     1621 ttacggaacg gatgccgtga ccggttggat cgccgatctt ttcccgtact tgagcgactc
     1681 cccagagcgg cgtcgcagcc acgtcttcgg attcgagcgc gaggattggg ccgtacccgt
     1741 tgaaaagggg gttaagactg aattttctct cggcgaaccc ggctccgaaa agggtgtagc
     1801 cccgaagagt ttcccttccg gcctggccag cgttccggtg aaactaagct ttccgaacgg
     1861 tttgaccagc ggcgtagact tggttgccgg ttttcttgcc gtcgagcagg atccggaaga
     1921 cctcgcgatt tctccggtga ttgggtggtc tgttactgag agcgccccca taaaacccgt
     1981 gcagatttaa gaacgtgttt tgaccgctat tgacaagtaa tagcaggcgg tttcctgttc
     2041 accggcgcgc ggaacaccag tcgttgaaca tccagggatg cctagccgac ttcccgttac
     2101 caatctcgtc gtgaggagct ctcgttacct cccggaaatc ccgtcatcat aaatcggcgg
     2161 ttcttctcgg gcacgtcctg aaggtaccgt gttgcccctg gccgtgcggt tttcgagacc
     2221 tgggagtctt catcacagaa ct
//
LOCUS       DASRKK010000674         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_163348, whole genome shotgun sequence.
ACCESSION   DASRKK010000674 DASRKK010000000
VERSION     DASRKK010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163348"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /gene="mnmG"
                     /locus_tag="VFC15_16375"
     CDS             complement(<1..1724)
                     /gene="mnmG"
                     /locus_tag="VFC15_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZM11784.1"
                     /translation="MRFTETYDVVVVGAGHAGCEAAMAAARMGLKTALFTLNVDLIAQ
                     MSCNPAVGGIAKGHLVREVDALGGIMGELTDAVGIQFRLLNTSRGPAVWSPRAQCDKQ
                     QYRLKMRELLESEPYLHIKQAEVAELIVEEVSSFEFRVSSKTGQQVATGNEQLETGNS
                     KLETSSIVRGVKLRDGRTVSAQAVIITTGTFLNGLIHCGEQQFPAGRSGEPSAVLLGE
                     SLKALGLRGCRLKTGTPPRLDGRSIDWSKFKVQPGDDDPTPFSFRTRRVAHHDKQVPC
                     YIAFTTPETHRIIRENVHRSPMYSGQIQSIGPRYCPSIEDKIVKFPDKETHQLFLEPE
                     GLNTHEIYVNGMSTSLPIDVQLAIIKSIPGLENAEMLRPGYAIEYDSIDPTELQRTLE
                     TKKIARLFLAGQINGTSGYEEAACQGIMAGINAALMVKGEPPLILDRTEAYTAILIDD
                     LISKGTNEPYRMFTSRAEFRLHLRIDNADRRLIPHGRRVGLINDAAWADHLAKQERMQ
                     AMRDLLERTRVNGEMLKVLKKEVSSESTERGQLENGNVNLDSDKLDGALGLTLAQLLK
                     RPEVQIERL"
     gene            1838..2821
                     /locus_tag="VFC15_16380"
     CDS             1838..2821
                     /locus_tag="VFC15_16380"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11785.1"
                     /translation="MNRNRSQISLALALAVLVSVGFICTAPAQSPTPVGKLPIAQLQK
                     LAESGDPAAENDLGVRYQLGFDVEKNPTLSEEWLHKAAKQGYGEALFNIGAASYNGDR
                     VAGTRRTAMYWFLLAEDAGDVSGKEAVAHMQSEMSPTDLNATYVLVGDGYEKGVDIKQ
                     DYVRAMQWYHKAADAGDAAACHRIALLYANGLGVPQDNVEIIRWLQRGADLGDPVALY
                     DLGRAYEQGNGVPQDFPKAAKLYQAAALYNNANAMFALGSLYNEGKGGKPEHQKALTW
                     FILAATYGNPDAGKQVTALSAKLNPIQVKKAKQDAVDYMWIQKRPLALIAK"
BASE COUNT          655 a          946 c          967 g          739 t
ORIGIN      
        1 agacgctcga tctgcacttc tggacgcttc aagagttgcg caagcgtcag gccgagtgca
       61 ccgtccagct tgtcagagtc aaggttcaca tttccattct caagttggcc gcgctcagtg
      121 ctttcactcg aaacttcttt cttcagcacc ttcagcatct caccattcac ccgggtgcgc
      181 tccagcaagt cgcgcatggc ctgcatgcgc tcctgcttgg cgagatgatc ggcccaggcg
      241 gcatcgttaa tcagtcccac gcggcgtcca tgcgggataa ggcggcggtc ggcgttgtca
      301 atgcgcaggt gcagccggaa ctcggcgcgc gaggtaaaca tgcggtacgg ctcattcgtc
      361 cctttgctga tgaggtcgtc gatgagaatg gccgtgtagg cctcggtgcg gtcgaggatg
      421 agtggcggct cgcccttcac catcagcgcc gcgttgatgc ccgccatgat cccctggcac
      481 gccgcttcct cgtaaccgct ggtgccgttg atctggcccg ccagaaacag gcgcgcgatc
      541 ttcttggtct ccaaggtgcg ttgcagctcg gtcgggtcga tggaatcgta ttcgatggca
      601 taaccggggc gcagcatttc ggcgttctcc aggccgggga tggacttgat gatcgcgagc
      661 tgcacgtcga tgggcagtga ggtgctcatg ccgttgacgt agatctcgtg ggtgttcagc
      721 ccctcgggct cgaggaagag ctggtgggtt tccttgtcgg gaaacttgac gatcttgtct
      781 tcgatcgatg gacaatatcg tggtccgata ctctggatct gcccgctgta catgggcgag
      841 cgatgcacgt tctcgcgaat gatgcggtgc gtctccggcg tggtgaacgc gatgtagcac
      901 ggcacctgct tgtcatggtg cgccacccgc cgggtgcgga agctgaaggg cgtcggatcg
      961 tcgtcgcccg gctgcacttt gaatttcgac cagtcgatgc tgcgtccgtc gagacgcggc
     1021 ggggtgccgg tcttcaagcg gcagccccgc aggccaagcg ccttcagcga ttcgcccagc
     1081 agtaccgcgc tgggctcgcc gctgcgcccg gcgggaaatt gttgctcgcc gcagtggatg
     1141 agcccgttca aaaatgttcc ggtggtgatg atgacggctt gcgcggagac cgtacggcca
     1201 tcgcgcagct tcacgccgcg aacgatggaa gaagtttcga gtttcgagtt tccagtttcg
     1261 agttgctcat taccagttgc tacttgctgc ccagttttgc ttgaaactcg aaactcgaaa
     1321 cttgaaactt cctcgacaat cagttccgcc acttcggcct gcttgatgtg gaggtagggc
     1381 tccgactcta gcaactcgcg catcttcaac cgatactgct gcttgtcgca ctgcgctcgc
     1441 ggagaccaca ccgctggccc acgcgacgta ttcagcagcc gaaactggat gccgactgcg
     1501 tccgtcaatt cgcccatgat gccgccgaga gcgtccacct cgcgcaccag atgtcccttg
     1561 gcaataccac cgacggcggg attgcacgac atctgcgcga tgagatccac attgagagtg
     1621 aacagcgcgg tcttcaggcc catgcgcgcc gccgccatag cggcttcgca accggcatgt
     1681 ccggcgccca ccaccacaac gtcgtaagtt tcggtgaatc gcactgagag gtatttcccc
     1741 aaataggaaa caagagacga tttgacctaa ttctatcaat tgggcagaag catttgggaa
     1801 ttccagtgct acgctgtggt gttcgccatt tttaaccatg aatcgcaacc gctcgcagat
     1861 ctcgcttgcg ctcgccttgg cagtgcttgt ttccgtcggc ttcatctgca ccgctccagc
     1921 ccagagtcca accccagtcg gtaaattgcc gattgcccag ttgcagaagt tggctgaatc
     1981 gggcgatccc gctgccgaga acgatctagg cgtgcgctat cagttaggct ttgacgttga
     2041 aaagaatccc acactttcgg aggaatggct tcacaaggcc gcaaagcaag gttacggcga
     2101 ggcgctcttc aatattggag cggcctctta caatggcgac cgtgttgcgg gaaccagacg
     2161 tacggcgatg tattggtttc tgctggcgga agatgccggc gacgttagcg gaaaggaagc
     2221 cgttgcgcat atgcagtccg agatgtctcc cacggatctg aatgcaacct atgtgcttgt
     2281 gggagacggc tatgagaaag gcgtcgacat caaacaggac tacgtccgcg ccatgcagtg
     2341 gtatcacaaa gctgcggacg ctggagatgc agcagcatgc caccgaatag cgttgctcta
     2401 tgcgaatggc ttgggagtac cgcaggacaa tgtggagatc attcgctggc tgcaacgcgg
     2461 tgctgatctt ggcgatcctg tcgccctcta cgatctaggg agagcttacg aacagggcaa
     2521 tggagtacct caggacttcc cgaaagccgc aaagctctac caggccgctg cgttgtacaa
     2581 caatgcgaac gcgatgtttg cgttgggcag tctttacaac gagggcaagg gcggaaagcc
     2641 ggagcaccaa aaagccctga cgtggtttat tctggccgcg acgtacggca atcctgatgc
     2701 tggcaaacag gtgacagctc tctctgcgaa actcaatccc atccaggtga aaaaagcgaa
     2761 gcaagacgcg gtagactata tgtggattca aaagagaccc cttgcattga tcgccaagta
     2821 gagttccagt ctgacgcgcc gcatatcggc tcatcacgtg ctttcttaga ccggttggtt
     2881 gctggcgaaa ctcttaaact ttgatgtagg tggccgaagg ccccgcatcc aagccctctc
     2941 tttttgtcat cccgtctata acgaggttgg tgttgttaag gcacacgacg gcgatgacgc
     3001 gctggcactt ggattttggt ttccttcttc cccaggattt ttgtttttga ctctgggctg
     3061 ggtttgcgaa atcggcattg aggacccgtc ttgtcggaat cgttaggtgg cagttttctg
     3121 gcgccaccat ccatctattc ggtcacctac ggcgaccatg attctccact tcgaactccc
     3181 cgcgatgggg gaggcgggct gcccacacaa ccgggcggga tcttaacgct ccccacggaa
     3241 tgacccgggc ttacccgcat gacgtgccag ctccgcgtac gtccattggt ggcggaggct
     3301 cctcact
//
LOCUS       DASRKK010000675         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_163421, whole genome shotgun sequence.
ACCESSION   DASRKK010000675 DASRKK010000000
VERSION     DASRKK010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163421"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..865
                     /locus_tag="VFC15_16385"
     CDS             500..865
                     /locus_tag="VFC15_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11786.1"
                     /translation="MMVEPVVQLDPGVDLQTAIATLIKVAENVEIVRWIELPASVLLF
                     LLVPGDPDSGAVYVLDRKKGTWYAVDFEDEQFGGYSVSQLEMLLKDCNFLDLIERPGL
                     WRTGLQWVVLPGMRPEATV"
     gene            979..2013
                     /locus_tag="VFC15_16390"
     CDS             979..2013
                     /locus_tag="VFC15_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11787.1"
                     /translation="MATTITATRQVVEDYQRLFVNRRAYTMQSMRPHPETGRHYYFRP
                     SKKGTDLPLLLTGETIRQHLEGKITVGLYAINPSTQRCKWVAIDADYKNSMEDLLKLQ
                     YHLTQDKVEPALEMSKRGGHLWIFLAKPLLARDCRVYIHDLARKLGVPVKGAGLAEGI
                     EVFPKHDAIGAGEFGNAIRGPLGIHRGANRRFWFYGADYTLEDQIAYLKRLRKVTEEE
                     LRRFITGKERPDPDVSLRPQRSIRAERKEGSARSEFRILEHVGTVRKVGRNYIARCPS
                     CADCGHDRSSDNLAILIEVCLVTILSGREINNFVGQPASGSAITLLPCLTCFGSGSEP
                     CFADFGHAAV"
BASE COUNT          460 a          580 c          587 g          429 t
ORIGIN      
        1 tcgatcgtct ggatgtagca gtctttggta gtctgagcgt aattgcgacg ctcgagttcc
       61 tcgagcatca ttttgcgaag gtgggtcata ggtcatctcc ttatgaccca accttattcg
      121 ttcacagcac agtgctgaca cggcgcacgc gaaagcgtct gccgcgaagc ggcttcgttc
      181 aaaccgcctt gggggagatg ccgaaccggg gtgccagcgg cattgttggt ggctcagaca
      241 ttacctttct gcgccatcgc cgattagtaa agagtctagc gtgcggactt gcatcctttt
      301 atatagagat cgtcctgcct agcgatcttc tttctgctct accccactct caaccctagt
      361 actgccaaca aaacaagata cttaacgcac tttcccctcc tccgccaact gacgtgtgcg
      421 tcatcggtcg catcttggct ttcaacacaa cacgactcgg gcggactgcc cggaaaggac
      481 cactgcaatg tttggacaaa tgatggttga acccgttgtg cagctcgacc ccggcgtcga
      541 tctacagaca gcgatagcca cactgataaa agttgctgaa aacgtcgaga tcgttcgttg
      601 gatcgagttg ccagcgtcgg ttctgctgtt cttgctcgtg cccggagacc cggattctgg
      661 tgcggtctat gtcctggacc gcaagaaagg aacctggtac gccgtcgact tcgaggacga
      721 acaattcggc ggctacagcg tgagccagct cgaaatgctt ctaaaggact gtaacttcct
      781 cgatcttatt gaacggccag ggctttggcg gaccggtttg cagtgggtgg tgttgcccgg
      841 aatgcggcct gaggcgaccg tttgacggaa aaaatattcc gggttatcta acgacaccca
      901 catccgaaaa ccgtgggggt aacaggcgac cacgagcgcg aggacacgcg aatctgccag
      961 aaggaggtga cgggtcaaat ggcaacgaca atcacggcaa cccggcaggt cgtcgaggat
     1021 taccaaaggc tgttcgtgaa ccgccgcgcg tacacgatgc agtccatgcg gccgcacccg
     1081 gaaacagggc ggcattacta cttccggcca agcaaaaagg gaacagacct acccctattg
     1141 ctgacgggag aaaccatccg ccagcacctg gaagggaaaa tcacggtggg cctgtatgcc
     1201 atcaacccct cgacccaacg ctgtaagtgg gtcgccatcg acgccgacta caagaactcc
     1261 atggaggacc tgctgaaact gcagtaccac ctgacacagg acaaggtcga gccggccctg
     1321 gagatgagca agcgcggtgg gcatctttgg atcttcctag caaagccgct actggcgcgg
     1381 gactgccgag tctacatcca tgatcttgcc cggaaattgg gagtgccggt gaagggtgcg
     1441 ggcctggcag agggcatcga ggtctttccg aagcatgatg ccattggcgc cggcgagttt
     1501 ggcaacgcga ttcgtggacc gctgggaatt caccgtggcg ccaaccgccg gttttggttc
     1561 tacggcgccg attacacgct ggaggaccag atcgcatacc tcaaacgcct gcgaaaggtc
     1621 actgaggaag aattgcggag gttcatcact ggcaaggaaa ggccagatcc cgatgtgtca
     1681 ctccggcctc agagaagtat tcgggctgaa cgaaaagaag gaagtgctcg ttcagaattt
     1741 cgcattttgg agcacgtggg taccgtgcgg aaggttggcc ggaactacat cgcgcggtgc
     1801 ccatcctgcg cggactgtgg ccacgaccgg agcagcgaca accttgccat cctgatcgaa
     1861 gtgtgcctcg tcacaatcct gtccggccgt gaaatcaaca acttcgtcgg gcagccggca
     1921 agcgggtctg ctataacttt gttgccatgt ttgacctgtt tcggctctgg ctcggagccg
     1981 tgcttcgcgg atttcggaca cgccgctgtc tgatgctcga aaacctcgcc cttcgccaac
     2041 aactcgccgt ctttaa
//
LOCUS       DASRKK010000676         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_652836, whole genome shotgun sequence.
ACCESSION   DASRKK010000676 DASRKK010000000
VERSION     DASRKK010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652836"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFC15_16395"
     CDS             <1..478
                     /locus_tag="VFC15_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HZM11788.1"
                     /translation="ALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEVVAIGKQL
                     LMTRGSLTTFSIANDVAKYFAIIPAMFAGTFPVLNALNIMHLRTPGSAILSAVIFNAV
                     IIIALIPLALRGVKYRALSAAVLLRRNLLIYGLGGIIIPFIGIKLIDMIITGVGLA"
     gene            697..1281
                     /gene="kdpC"
                     /locus_tag="VFC15_16400"
     CDS             697..1281
                     /gene="kdpC"
                     /locus_tag="VFC15_16400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZM11789.1"
                     /translation="MKQNLKIAILMTIVTTVLLGLIYPLVVTGLAQLLFRDKANGQLV
                     SRNGQIVGSRIIGQAFVGPGYLHSRLSAAGAGNGYDAQNSGGSNLGPTNQHLIDRVKA
                     DVARLQAENPGQPVPVDLVTTSASGLDPDVSPAGAEFQVPRIARERGLSEDQVRQIIS
                     QHTLGRQIGFLGEPRVNVLEVNLALDQVKAMASR"
     gene            1596..2936
                     /locus_tag="VFC15_16405"
     CDS             1596..2936
                     /locus_tag="VFC15_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZM11790.1"
                     /translation="MAETIIQACAVEKYYPQADGNRIEVIAPTDLSVSPGKIVALLGP
                     SGSGKSTLLRMLTGLSKPSSGQVLWHDRPLDGHIPNVSIVFQSFALFPWLTVLENVEA
                     PLIARGAGAIERHKRALKMIDTVGLDGFETAYPKELSGGMKQRVGFARALVVEPEVLF
                     MDEPFSALDVLTAENLRSELLELWLSKKMPTTAIFIVTHNIEEAVLLADRVIVLGRNP
                     GRIRSDFEIDIPQYRDRKSPRFLAYVDYIYKTLTRPEEEASAPGKLAGQSADGQPRTR
                     TKFQMLPHARPGGIAGFMELLADRDGSEDLYKLADELSMDVDDLLPIVDASVMLGFAE
                     LHEGDVATTPEGRAFGEADIQGQKAIFRRAALEHVALLRLIENTLHAKRDRAIGEEFF
                     RDILDEHFSADEVERQFETALNWGRYAEIFDYDSESKRLFHNEEADTVPSLGNR"
     gene            2936..>4063
                     /locus_tag="VFC15_16410"
     CDS             2936..>4063
                     /locus_tag="VFC15_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZM11791.1"
                     /translation="MARSTWQPIRILGKGPWSLLPAQTRSVLTDFVIFAAGLSVFYVI
                     LLLGRTWLGPFNPHVEISRDPTKLPMYAAYSLLRIAVAYVLSLAFALVYGYVAAYNRK
                     AERIMIPLLDILQSIPVLSFLPGVMLAMVALFPHHQIGVEMGAILLIFTGQVWNMAFS
                     FYSSLKSIPRDMREAAQIYRFNWYQRFTQFELPYAAIGLVWNSMMSVAGGWFFLMACE
                     MFVLGSRDFRLPGLGSYLQTAASAGDTQAIFWGVGVMIAVIVLLDQIVWRPVIAWAEK
                     FKMEQVESADVPHSWFLNVIQGSRGLVRLRKRAIRPLQERLAMHFARQHDSGAEDAPP
                     VPWKVWTGRAIASIAVVAVLYAVTKAIAMLTGLDGSEYRELL"
BASE COUNT          786 a         1177 c         1235 g          865 t
ORIGIN      
        1 ggcgctggcg caggctgacg tcggtgtagc catgaacacc ggtacccagg cagccaagga
       61 agccggcaac atggttgact tggattccaa tccgaccaag cttatcgagg tggtcgctat
      121 cggcaagcag ttgctgatga ctcgcggatc gttgaccaca ttttccatcg ccaacgacgt
      181 cgccaagtat ttcgccatca ttccagccat gttcgcggga acgttccccg tgctgaacgc
      241 gctcaacatc atgcacttgc gcacacctgg atccgccatc ctgtcagccg tgattttcaa
      301 tgccgtcatc atcattgcgc tgattccgct ggcgttgcgc ggggtgaagt accgggccct
      361 tagcgcggcc gtcctgctgc ggcgcaacct gctgatctac ggtttgggcg gcatcatcat
      421 tccgttcatc gggatcaagc tgattgacat gattatcacc ggcgtgggac tggcgtgaag
      481 acttttcacc acggagagca cggagggaaa taagaaagag aagactatgg actccatgcc
      541 tcggtaatgg acagacgctt ttcgagtgct ttgttaacgg tgccgcttgc gagctgattc
      601 gtaaggacta ggtacacggt ttagatacag gaaatcagaa ctccgtgcac tccgtgtcct
      661 ccgtggtgga aaaaggcgtc ggcagaagaa gggattatga aacagaatct taagattgca
      721 atcctgatga ccatcgtcac gacggtgcta ttgggactca tctaccccct ggtggtcacg
      781 gggctggcgc aactgctgtt ccgcgacaaa gccaacgggc agctcgtttc gcgcaatggc
      841 caaatcgtcg gttcaaggat tatcgggcag gctttcgtcg ggccgggata cctgcactca
      901 cggctatcgg cggcaggcgc gggaaacggc tacgatgcgc agaactccgg cggatcgaat
      961 ctgggtccga cgaaccagca tttgatcgat cgcgtgaagg ccgatgttgc gcgtctgcaa
     1021 gcggagaacc ctggccagcc ggttcctgtg gacctggtta cgacctctgc gtcaggtctg
     1081 gatcccgatg tctctcccgc tggagcggag tttcaagtcc cgcgcatcgc ccgcgagcgc
     1141 gggttgagcg aggaccaggt ccggcagatt atcagccagc acacgttggg acggcagatt
     1201 ggtttcctgg gcgagcctcg ggtgaatgtg ctggaggtaa acctcgcact cgaccaggta
     1261 aaggcgatgg cctcgagata gcgacagggt tagaccagtt tcagtgggcg tagaccggta
     1321 gagccggcct ttgggccggc gtccaaggtc gcctcgttct atggggctcc agcggctcaa
     1381 gcggttcctc ttttccgcgg cgaacgcggc accgaatcgc agcaagcttt gaagccgtgc
     1441 cccttcaagc gtggggtcct cgcggacggc agcccagaat gggcctttga gcctcaccaa
     1501 attcacactc ctttcacaag aatcgtatgc ctcccgattg tctgcaccag cgcgcaaccc
     1561 atagactaag aaaatcaggg cacgggagat gggatttggc tgagacaatc attcaagcct
     1621 gtgcggtaga gaagtactat ccgcaagccg acggcaatcg catcgaggtg attgcaccca
     1681 cggatctcag cgtgtctccg ggaaagattg tggctttgct tggtccgtca ggctcgggca
     1741 aatcgacgtt gctgcgcatg ctgaccggcc tgtcgaaacc gtcctccggg caggtgctat
     1801 ggcatgacag acctctggac gggcacattc cgaatgtgtc catcgtcttc cagagcttcg
     1861 ccctctttcc ctggcttacc gttctagaga acgtggaagc tccgctcatc gcgcgaggtg
     1921 ccggtgcaat cgagcgccac aagcgggcgc tgaagatgat cgataccgtc ggtctcgacg
     1981 gattcgaaac tgcctaccca aaagaactgt cgggcggaat gaagcagcgc gtcggcttcg
     2041 cgcgcgcgct ggtggttgag ccggaagtgc ttttcatgga cgagccgttc tcggccctcg
     2101 atgttctcac cgccgaaaac cttcgctccg agttgctgga gttgtggctt agcaagaaga
     2161 tgcctaccac cgccatcttc attgtgaccc acaatatcga ggaagccgtg ctgctggcgg
     2221 accgggtcat cgtgctcggg cgaaatcccg ggcgcatccg ctcggacttc gagattgata
     2281 ttccgcagta tcgcgaccgt aagtcaccgc gctttttggc ttatgtggat tacatctaca
     2341 agacgctgac gcgtcccgaa gaggaagctt ctgcccccgg gaaactcgcc gggcaatccg
     2401 cagatggcca gccgcgtacg cgcaccaaat ttcagatgct gccccatgct cgccctggag
     2461 ggattgcggg gttcatggag ttgctggccg accgcgacgg atccgaagac ctctacaagc
     2521 tggccgacga actgtccatg gatgtggacg atcttctccc catcgtggac gcctcggtga
     2581 tgttgggttt tgcggaattg catgaaggcg atgtcgcgac tacgccggag ggccgtgcct
     2641 tcggggaggc cgacatccag ggacaaaagg ccatcttccg cagggcggcg ctggaacacg
     2701 ttgcgcttct gcggctcatc gagaatacgc tgcacgccaa gcgcgaccgc gcgatcggtg
     2761 aggagttctt ccgcgacatc ctcgacgaac acttcagcgc cgatgaagtg gagcgccagt
     2821 tcgagaccgc actgaactgg gggcggtacg ccgagatctt cgactacgac tctgaatcca
     2881 agcggctgtt ccacaatgaa gaggcggaca cggttccctc gctaggaaat cgctgatggc
     2941 gcgcagtacg tggcagccta tccggattct gggcaaaggc ccgtggtcgc tgctgcccgc
     3001 gcagacgcgg tcggtgctga ctgacttcgt gatctttgcc gccgggttgt cggttttcta
     3061 cgtaatcctg ctgctgggac gcacctggct cggccccttc aatcctcacg ttgagatttc
     3121 acgcgatccc accaagctac caatgtacgc cgcgtactcg ctactgcgga tcgcggtcgc
     3181 ctatgtactt tcgctcgcct tcgcgctggt gtacggctac gtcgctgctt ataaccgcaa
     3241 agccgagcgg atcatgattc cgctgctgga tattctgcag tcgatcccgg tgctcagttt
     3301 cctgccggga gtgatgctgg ccatggtggc gctgtttccg catcatcaga tcggcgtcga
     3361 gatgggcgcc atcctgctga ttttcaccgg gcaggtatgg aacatggcct tcagcttcta
     3421 ttcgtcgttg aagagcatcc cgcgcgacat gcgcgaagcc gcccagattt accgcttcaa
     3481 ctggtatcag cgattcacgc agttcgagct tccgtatgcc gccattggct tggtatggaa
     3541 ttcgatgatg tcggttgcgg gcggctggtt ctttctcatg gcctgcgaga tgttcgtgct
     3601 gggctcgcgc gacttccgcc tgcccgggct gggttcgtac ttgcaaaccg cggccagcgc
     3661 gggcgacacc caagctattt tctggggcgt cggcgtcatg atcgcagtga ttgtgttgct
     3721 cgaccagatc gtgtggcgtc cggtcatcgc ctgggccgag aagttcaaga tggaacaggt
     3781 ggagagcgcc gacgtgccgc actcctggtt cctcaatgtc atccaaggat cgcgaggact
     3841 ggtccggctg cgcaaacgtg cgatcaggcc attgcaagag cgcctcgcga tgcacttcgc
     3901 gcggcagcat gacagtggtg cggaagatgc tccgccagtg ccgtggaagg tgtggaccgg
     3961 tcgcgcaatc gccagcattg ccgtggtcgc ggtgctctac gccgtgacca aagccatcgc
     4021 aatgctgacc ggcttggatg gctcggaata ccgcgaactg cta
//
LOCUS       DASRKK010000677         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_164099, whole genome shotgun sequence.
ACCESSION   DASRKK010000677 DASRKK010000000
VERSION     DASRKK010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164099"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2673
                     /locus_tag="VFC15_16415"
     CDS             <1..>2673
                     /locus_tag="VFC15_16415"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZM11792.1"
                     /translation="TTQGQTDASGKAQIKLTIPDAGEFRVRVTATTPENRTVETTAFL
                     WAPGESPWWSGPAQERVQIVPDKKTYQPGDTAHVLIVTGKEPTSVLVTVEGNGLYSGQ
                     VIKSSGGSLTVDVPIKPEYAPNFYVAAVFIRGNKLYQGSKSLTVPPIQHELNVQLLPS
                     KPQYQPGEAASYTIKATDAGGKPVAAEFSLGVVDEAIYAIKPETVGSIVNAFYGRVYS
                     RVSTETSLTYYFSGQAGKRAMQLANVRSSKAMAQLKPERLVQPKIRKAFPDTAYWMAD
                     VRTGSNGQATVHFDYPDAITSWRATTRGVTQDTKVGSAVVNTIVRKNLMVRLVVPRFF
                     RRGDEITISTIVQNYLPTAKVAQVSMVFDGLQVLDGGQQDVTVPSRGLMKVDYRVRVL
                     NVDSARVLGKALTDVESDAMELTLPVVPFGVKLAMAKAGSVAGAGTSDTVQPLTFPAG
                     AEVGTRKLTIGVTPSIAGTVFAALDFLTSYPYGCTEQTMSSFLPDVLVANSLKKLGVK
                     SNIDPQVLSKQVQAGLDRLYNYQHPDGGWGWWQTDDSQAFMTAYVLAGLSQAKAAGFD
                     VKQDAIDRGRSWLRPQFDRSEKVKTDLRAYMAYALVLSGTREAAVLDSVWAQRSTLTA
                     YGQALLGLAMLEMNDGRANELSKQIEAEVKQDDSQAWWPSDNNYLLDFSADTTPETTA
                     YALKLLGHTDEASPLLSKAAVYLVNHRDQGYYWESTEQTAMVIYGLTDYLERTKELQP
                     NYSVEVQVNGKIVATKKFTAADALAPATTVVLNESQLAPGTNQIRIAKSGDGRLYWST
                     RGEYYSSESKVVNTGNFKLSVVREYYRLSQQQKDGRVVYHLDKLAGPVQVGDTLAVRL
                     SVGGNNWKYLMIEDPIPSGTESITRDDLYELDERP"
BASE COUNT          590 a          749 c          860 g          474 t
ORIGIN      
        1 cactacgcag ggccaaaccg acgcgagcgg caaagcacag atcaagctca ccatccccga
       61 tgctggcgag ttccgtgtcc gcgtgaccgc aaccactccg gagaatcgca cggttgagac
      121 caccgcattc ctgtgggctc ctggtgaaag tccatggtgg agcggtccgg cgcaggagcg
      181 cgtgcagatt gtcccagata aaaagacata tcagccaggt gacacggcgc atgtgctgat
      241 cgtcaccggc aaggagccga cttcggtcct ggtgacggtt gaaggcaacg gactctactc
      301 cggtcaggtg atcaagagca gcggcggcag cctcacggtg gatgttccca tcaagccgga
      361 gtacgccccg aacttctacg tggcggcggt tttcattcgt ggcaataagc tgtaccaggg
      421 gtccaagagt ctcaccgttc cgcctataca gcacgagctg aatgtgcaac tgctgccttc
      481 caagccgcag taccaaccgg gcgaagcggc gagctacacg atcaaggcga cggatgccgg
      541 cggcaagccg gtggcggcgg aattcagttt gggcgtggtg gacgaggcta tctacgcgat
      601 caaaccggaa acggtgggca gcatcgtgaa cgccttctac ggccgcgtct attcgagggt
      661 gagtactgag acctcgctga cgtattactt cagcgggcag gcagggaaac gagcgatgca
      721 gttggccaac gttcggtcgt cgaaagcgat ggcgcaactg aagcctgagc gcctggtgca
      781 gcccaagata cgcaaggcat ttcctgacac cgcgtactgg atggcggacg tgcgcaccgg
      841 cagcaacgga caagccacgg tgcacttcga ttatcccgac gccataacgt cgtggcgcgc
      901 gaccacccga ggtgttacgc aagacaccaa agtcggcagc gcggtggtaa acacaatcgt
      961 gcgcaagaac ctgatggtgc gtctggtagt gccgcgcttc ttccgccgcg gcgacgagat
     1021 aacgatctcg accatcgtgc agaactattt gcccacggcc aaagttgcac aagtctcgat
     1081 ggtatttgac ggccttcaag tgctcgatgg tggccagcag gatgtcaccg tcccaagccg
     1141 tggattgatg aaagtggatt accgggtgcg cgtgctcaat gtggattcgg cgcgggtgct
     1201 gggcaaagcg cttaccgacg tcgagagcga cgcgatggaa ctgacgctgc cggtcgttcc
     1261 cttcggcgtg aagctggcga tggcgaaggc gggatcggtc gcaggcgcgg gcacgagcga
     1321 cacagtgcag ccgttgacct tcccagcagg cgcggaggtg ggaactcgca agctcacgat
     1381 tggcgtgacg ccctccatcg cgggcacggt cttcgccgca ctcgacttcc tgacctccta
     1441 tccctatggc tgtaccgaac agaccatgtc gagcttcctg ccggatgtgt tggtggcaaa
     1501 ctcgctgaag aagctggggg tgaagtcgaa catcgatccg caagtgctga gcaagcaggt
     1561 gcaggcgggt cttgaccggc tctacaacta tcaacacccc gacggcggct ggggctggtg
     1621 gcagactgac gacagtcagg catttatgac ggcatacgtg ctcgccgggc taagtcaggc
     1681 gaaggcggca ggatttgacg tcaagcagga cgcgatcgat cgaggacgga gctggttgcg
     1741 cccgcagttt gatcgctcgg agaaagtgaa gaccgatctg cgcgcctata tggcgtacgc
     1801 gctggtgttg agcggaactc gtgaggcggc ggtactcgat tcagtgtggg cacagcgctc
     1861 gacgttgacc gcctacggcc aggcgctcct cggactcgcc atgctggaga tgaacgatgg
     1921 ccgggccaac gagttgagca agcaaattga agccgaggtg aaacaggacg actcgcaagc
     1981 ctggtggcct agtgacaaca actatctgct ggacttctcc gccgacacca ctccggagac
     2041 gacggcgtac gcgctgaaac tcttaggcca caccgatgaa gcgagcccgc tgctgtcgaa
     2101 ggccgcggtt tacctggtga accatcgcga ccagggctac tactgggagt caactgaaca
     2161 gacggcgatg gtgatctacg gactcaccga ttatctggaa cgcacgaaag agttgcagcc
     2221 caactacagc gtcgaggtac aagtgaacgg caagatcgtg gcgaccaaga agtttactgc
     2281 tgccgacgca ctggctcccg ctacgacggt tgtgctgaat gagtcgcaac tggcgccggg
     2341 aacgaaccag attcgaatcg cgaagtcggg tgacgggcgg ctgtactggt caactcgcgg
     2401 agagtattac tcgagcgagt cgaaagtggt gaacaccggc aacttcaagc tgagcgtggt
     2461 gcgcgaatat taccggctgt cgcaacaaca gaaggacggg cgcgtggtgt atcacctcga
     2521 taaactcgcg ggaccggtgc aggtcggcga cactctggcg gtgcgcctca gcgtcggtgg
     2581 taataactgg aagtacctga tgatcgaaga tccgattcca tcggggaccg agtcgatcac
     2641 gcgggacgac ttgtacgaac tggatgagcg tcc
//
LOCUS       DASRKK010000678         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_555527, whole genome shotgun sequence.
ACCESSION   DASRKK010000678 DASRKK010000000
VERSION     DASRKK010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555527"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1219
                     /locus_tag="VFC15_16420"
     CDS             188..1219
                     /locus_tag="VFC15_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028714619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM11793.1"
                     /translation="MQTITTIGLDIAKSVFQVHGVDAAGQVVIRRQLKRRSVLAFFQK
                     LPSCLVGIEACASSHHWSRELRALGHTVRLMPPAYVKPYVKRQKNDMADAEAICEAVA
                     RANMRFVPTKTPEQQSCLMLHRTRHLFIRQQTAVINSIRAHLAEFGIVAPVGRKGVTE
                     LLHIVADPSDTRVSEVARACLAALGGQLLGLKQQIAEFDRMILAWHRSNQTSKRLHYI
                     PGVGPMLATALVASVADPSTFRSARNFSAWIGLVPKQYSSGGKERLGSISKQGDRYLR
                     SLFVAGALAVIRYAKIHGTGHRPWLTALLARKPTKVAAIALANKIARMAWAMMAKGER
                     YREPIALAR"
     gene            1678..2712
                     /locus_tag="VFC15_16425"
     CDS             1678..2712
                     /locus_tag="VFC15_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM11794.1"
                     /translation="MQITTIGLDLAKNVFQVHGVDATGQVVVRKSLRRSQMLPFFAKL
                     PPCLVGIEACGTSHHWARELVKLGHEVRLMPPAYVKPYVKRGKTDAADAEACCEAVTR
                     PTMRFVPVKSPEQQAVLSIHRARDLLVKQRTQLINMIRGLLAEFGVDIPKGLERALLM
                     ARQIVDGKAPAVPIEASKIVATLSQQALDIHVRLREIDRDLAVCLRGNDVARRLLTIP
                     GIGPIGATALAASVTDPHQFRSGRQFAAWLGLTPLQKSSGGKERLGRISKMGDKYLRK
                     LLIVGMTSLVGRAKCNPGKIDPRLADLLARKPTRVATVAMANKTARVIWAIMVRGETY
                     RTGHQPVLAA"
BASE COUNT          644 a          895 c          947 g          597 t
ORIGIN      
        1 gattaatctc aagaccgcga aagcgctcga tctcagggtg ccagactcgc tcctcgtcca
       61 agcgactgag gttatggagt gaacattccc acttccgctt gtgatgctgt ggacggctcc
      121 tccaccggca catgagtgcc acggatgtgg gtgctgttga ggctcccacg attcagagga
      181 gcgagccatg cagacgataa cgacaatcgg tttagacatc gccaagtcgg ttttccaggt
      241 acacggggtt gatgctgcag gccaggtggt catccgccgc cagttgaagc ggcggtctgt
      301 gctggcgttc tttcagaagc tgccatcatg cctggttggc atagaggctt gcgcctcgtc
      361 tcaccattgg tcgcgcgaac ttcgggcgct cggccatacc gttcggctga tgccaccggc
      421 ctacgtgaag ccctatgtaa agcggcagaa gaacgacatg gctgacgcag aagctatctg
      481 cgaggcggtc gcccgagcca acatgcgctt cgtgccgacg aagacgcctg aacagcagag
      541 ctgcctgatg cttcatcgta cccgccatct cttcatccga cagcagaccg cggtgatcaa
      601 ttcgatccgg gcgcatcttg ccgagttcgg catcgtcgcg ccggttgggc gcaaaggagt
      661 gaccgagctt ctgcatattg ttgccgaccc gagcgacacg cgggtgtcag aagttgcccg
      721 cgcgtgtctt gctgcgctag gtggtcagct gctcggcctc aagcaacaga ttgcggagtt
      781 cgatcgtatg atcctggcct ggcatcggtc taaccagacg agcaaacgcc ttcattacat
      841 tcccggggtg ggtccgatgc tggcgaccgc tctggtcgcc agtgttgccg accccagcac
      901 cttccggtcg gcgcgtaatt tctcggcctg gatcgggctg gttccgaagc agtactcaag
      961 cggaggcaag gaacggctcg gcagcatcag caaacagggt gatcgctatt tacgcagcct
     1021 gttcgtggcc ggagcactcg ctgtcatccg ctatgccaag atccatggca ccgggcatcg
     1081 gccctggctc acggcgttgt tggcgcgaaa gccgaccaag gttgccgcca tcgcgcttgc
     1141 caacaagatt gcgcgaatgg cctgggccat gatggccaag ggtgagcgct acagggaacc
     1201 catcgcgctt gcgcgataaa cgagatcgcg ccggacaacc ggcgtgatgt gaaggttggg
     1261 agggcgaaca gcacgtaatg cagagccggt cgatccggcg gtcaggacaa cccactcgtg
     1321 ccatcgcatt actatcgaat gcgtgttttt ggtcgggacc tgatccgcgg agggcattat
     1381 ggccagcggt catgtgaacc gcatccaaag gccgaacaca tggctgcacc gaccaatcct
     1441 gcaaaacgtg aaaaaagccc ttgccaatcc ggagccgtcc acacatggca cgaaacggac
     1501 atggcgtgat gtccgacttg agtctgcttt cgggggtata gcggaagtcg cgtttcagag
     1561 ccgtcaagtc agcttttgac ccatctgaga tgaggtggat ggctcccgct cacggcatct
     1621 gggtgccaaa gtgtgaaggc catcaggcgt tcacgagcaa aatgggagcc atccgctatg
     1681 cagattacca cgatcggcct cgacttggcc aagaacgttt ttcaggtcca cggtgttgat
     1741 gcgaccggcc aggttgtggt ccgcaagtcg cttcggcgct cgcagatgct gccatttttt
     1801 gccaagttgc cgccttgcct ggtcggcatc gaggcgtgcg gcacgtcgca tcactgggcg
     1861 cgcgagctgg tcaagcttgg ccatgaagtg cgcctgatgc cgccggctta tgtaaaaccg
     1921 tatgtgaagc gcgggaagac cgacgctgcc gatgcggaag cgtgttgtga ggcggtaaca
     1981 cggccgacga tgcggttcgt gcccgtcaaa tcgccagagc agcaagcggt gctgtcgata
     2041 caccgggcac gcgatctgct ggtcaagcag cgcacgcagc tgatcaacat gatccgcggt
     2101 ctactggccg aatttggcgt cgacatcccc aaggggctgg aacgtgccct gctgatggcg
     2161 cggcagatcg tcgatggcaa agcgcctgcg gtgccgattg aagcttccaa gatcgtcgcc
     2221 acgctgtcac agcaggcgct cgacatccat gtccggctcc gcgagatcga tcgcgatctg
     2281 gccgtttgtt tacgtggcaa tgatgttgca cggcgtctcc tgaccatccc cggtatcggt
     2341 ccgatcggcg cgacggcgct tgccgcatca gtcaccgatc cgcatcagtt ccgttcgggt
     2401 cggcagttcg ctgcctggtt gggactaacg ccacttcaga agtcgagcgg gggcaaagag
     2461 cggctcggtc gtatcagcaa gatgggcgac aaatacctgc gaaagctgct cattgttggt
     2521 atgacctcgc ttgtaggccg tgcaaaatgc aatcccggga agatcgatcc acgcctggcg
     2581 gatctgctgg cgcggaaacc cacaagggta gccaccgttg cgatggcgaa caaaaccgca
     2641 cgggtcatct gggcgatcat ggtgcgtggc gaaacctacc gtacaggcca ccagccggta
     2701 ctggcagcat aaacgatata ggatttagct gaagaggaga tcagcttcac gaggttgcga
     2761 gcgcgatgcg atgtgatggc aaaccggtca gaccgggaac agggacaacc caaccgatgt
     2821 ccaaggcgtc atagcctgaa actcagaacg ggaccttgtt cgcggactcc atcagggcca
     2881 gcagtcccta ccgactgcat caacaggccg gacagaagac tgcacccgac caacaagccg
     2941 aatcatcaaa ttgtgcttgc aacgcgggag ccatccacag aggacacgct ggccagagaa
     3001 gtgggtggaa atctgccgtt cattcgcccc tatttgaacg gcgaggtcgt gccgaaccgg
     3061 acttcacgag gttaggcgca ccc
//
LOCUS       DASRKK010000679         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1142167, whole genome shotgun sequence.
ACCESSION   DASRKK010000679 DASRKK010000000
VERSION     DASRKK010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142167"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            628..897
                     /locus_tag="VFC15_16430"
     CDS             628..897
                     /locus_tag="VFC15_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11795.1"
                     /translation="MPVEDPKVEKPAEHFDKPKDVVKDVDLSKREKTDALDTWEQDAR
                     QRLTASNEGMTGPAEGSDKKDSHRLDEVIKAKQTIGETPNSKPAH"
     gene            1027..2883
                     /locus_tag="VFC15_16435"
     CDS             1027..2883
                     /locus_tag="VFC15_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZM11796.1"
                     /translation="MLLAALGVVYGDIGTSPLYAFRESLKSAGGSVAATEAAVLGILS
                     LIVWTLVIVVTLKYVLFVMRADNQGEGGIMALLALALPEVSEKGTRKFLLVVGVGGAA
                     LLYGDGMITPAISVLSAVEGLSFATTFFEPYIVPITLVILIALFAVQSYGSGPIGRLF
                     GPIMAIWFLTLGLAGFWHILAQPSVLAALNPAHAISFLGGNGWTAVIVLGSVFLVVTG
                     AEALYADMGHFGRRVIRVDWFILVLPALALNYFGQGALVLANPQAAANPFFLMFPSWL
                     LYPIVVLATAATVIASQALISGAFTLSQQATLLGLLPRLEIRQTSSSAIGQVYVPQIN
                     WLLAGSVVALVLGFRTSGALANAYGIAVVSTMIATTVLAGVVAHHRWGWSLPLVLTVT
                     GAFLIIDLIFLVANAPKIPHGGWFPLIAGALIFTIMMTWRRGRKVVLERLEEDQAKLS
                     EFFEKLDDRQLPRVKGTAVYLSARRDTVPSALALNLRHNKVLHDRVLLLNVEVLRVPF
                     VTEKSRVVIEALPKGFLGVTVRFGFYEKLDVLDALNRHTKAARGDGSFTFELAETTFF
                     LGREVPVPTVRPDLSGWQTAIYTFLTRNAVSAPEYYLIPTENVVELGTRVEI"
     gene            complement(3248..>3796)
                     /locus_tag="VFC15_16440"
     CDS             complement(3248..>3796)
                     /locus_tag="VFC15_16440"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZM11797.1"
                     /translation="IAGVAGLVAGAMAMAAGEYVSVSSQSDSERADLARERKELSENP
                     AFELDELAEIYVKRGVDQVLARQVAQQLMAKDALGAHARDELGISKTTTARPVQAALA
                     SAASFSVGAAMPLLMVVVSPTGALVPIVFAASLCFLALLGAIGAKVGGAKILRAMVRV
                     TFWGALAMALTAGIGKLFGTVV"
BASE COUNT          800 a         1096 c         1067 g          833 t
ORIGIN      
        1 agccgccgcc gacatgcgcc cgcctgccaa tgtagaaccg tccaggacgg ctgtaatccc
       61 gcatcagcgg cacctacaat cggaaaagcc gctaaacgtg ggaaaatggc cacgcatggc
      121 cacttagccg aggttgcgat ccgtctatct ttccgtcagg gcgttttcac acgaccaaga
      181 cccaaagcgg acttaggcat tgggccggaa acagtgtagt gttgctcaga cgccgctacc
      241 cgcagccctc ccactgttcg ccaccggcct cggcgcgttg gtgttgctag ggcgacgtag
      301 gaataggaaa cagaaacaga ctgcggcggc ctgagcgcgg ccacgcttgc tagggaagga
      361 ccgccgcgag gcgctctttc tttttgcgct caaggatcaa agcggatttg gctgacggca
      421 gctattcgca cgaagcggct acggcaggct ttcgagaatc atcgaattgg gtttttagaa
      481 gttaatggcc ttcgaattcg tcccctataa tcagggcgtt ctgaagctgg gctcgcccct
      541 ggccatatcg agctattcat taaatgcagc tgcaagtggg aacccgaaac gcatttgcac
      601 gttgaacacc acacccagga ggctttcatg ccagtcgaag atccaaaggt cgaaaagccc
      661 gctgagcatt tcgacaagcc caaggatgtc gtgaaagacg tcgacctttc caagcgtgag
      721 aaaacagatg cgctggatac atgggaacag gatgctcgcc aacggctcac cgccagcaac
      781 gaagggatga ccggacctgc ggagggctca gacaaaaaag atagccatcg tcttgatgaa
      841 gtgattaaag cgaaacaaac aatcggcgaa acgccaaatt ccaaacctgc ccattgattt
      901 atctgtgttt acaaaaaacc tgaagcttgg cctgcccgtt cgtaagcatt gtttcgaacg
      961 cctatgaatg gaacacctat ccccgattca gccggcggcc gtgcctcttc aggggcgcgg
     1021 ccggcaatgc tgctggctgc cctcggtgtt gtttatggtg atattggcac gagcccactt
     1081 tacgcttttc gcgagagtct caagtctgca ggtggaagcg tagcggcaac agaagctgcc
     1141 gtgcttggaa ttttatcatt gatcgtctgg acgttggtta tcgttgtcac gctgaaatat
     1201 gtgcttttcg ttatgcgtgc ggataatcag ggcgagggcg gcatcatggc gcttttggcg
     1261 ctcgccctgc ccgaagtgag cgagaaaggg acgcgcaagt ttcttcttgt cgtgggtgtc
     1321 ggaggcgccg ctctccttta tggagatggc atgatcacgc cagcgatctc cgtgctctcg
     1381 gctgtagagg ggctttcttt tgcgacaacg ttcttcgagc cctacattgt tccgatcacc
     1441 ctcgtcatcc tcatcgcgct cttcgctgtg cagagttacg gcagtggtcc gattggccgg
     1501 ctgttcggcc ccattatggc gatatggttc ctgacgctcg ggcttgctgg tttctggcac
     1561 attctcgcac agccaagcgt actcgcggcc ctcaaccctg cacacgcaat tagtttcttg
     1621 ggcggaaacg gctggaccgc cgttatagtg ctaggttcgg ttttcctcgt cgtcaccggc
     1681 gcggaagccc tttatgcaga catgggccat ttcggccgac gcgtaatacg ggtggattgg
     1741 ttcatcctgg tgcttccagc actagcattg aactatttcg gacagggcgc gctcgtactc
     1801 gccaatccgc aagccgctgc caacccgttc tttttgatgt tcccctcctg gctcctatat
     1861 ccaatcgtgg tgctcgccac cgccgccact gttatcgcaa gccaggcgct tatctccggc
     1921 gctttcacac tcagccaaca agcaacgcta ttgggacttc tgcctcggct cgaaatccgc
     1981 cagacctcct cgtccgctat cggccaagtc tatgtgcccc agatcaactg gctgctggct
     2041 ggctccgtgg ttgcgctggt actcggattc cgcacgtcgg gagcgcttgc aaacgcctac
     2101 ggtatcgccg tcgtgtcaac gatgatcgct acgaccgtgc tggccggcgt cgttgctcac
     2161 catcgttggg gttggagctt gccattggtg ctaaccgtaa caggcgcctt cttgatcatc
     2221 gacctgatat tcttagtcgc taatgcgccc aagattccgc acggcggttg gtttcctctg
     2281 atagccggtg cattgatttt cacgatcatg atgacgtggc gacgtggtcg aaaagtcgtc
     2341 ctggagcgtc tggaagagga ccaagcgaag ctctcggagt tttttgagaa acttgatgac
     2401 cgacagctac ctcgcgtgaa gggcacagcg gtgtatctca gcgcaaggcg cgacacggtc
     2461 ccttctgcgc ttgccctgaa cctgcggcac aacaaggtgt tgcatgaccg cgtcctgctt
     2521 ctgaatgtcg aggtccttcg tgtcccgttc gtgactgaaa aaagccgggt tgtaattgaa
     2581 gctttgccaa aggggttctt gggcgtgacg gtgcgctttg gcttctacga gaagctggat
     2641 gtgctagacg cgctcaatcg acacaccaaa gccgctcgtg gtgatggcag ctttaccttc
     2701 gagcttgcgg aaacaacatt ctttcttggt cgagaggtgc cggtgccaac ggtccggccc
     2761 gatctgtcgg gctggcaaac ggcaatctat acgttcctga cgcgaaacgc ggtaagcgca
     2821 cctgaatact atctcattcc gaccgagaac gtcgtcgaac tcggaacccg ggtcgagatc
     2881 tgatttctta tcgtgcagaa gggtaatggc tacggccggt ggctccgcgc tacgagcaag
     2941 aggtggcctg cgcgatcaat gaactccggg ctcgtggaat atgtctcttc gagtttcacg
     3001 agctcatctg aaagctgcct atcggctcgc aaggatgcgg gattccaccg aagttgctcg
     3061 cgctcgccga cgaggtgatc gaatagcgct gcaaattgct gcggtgcatg agtccgctta
     3121 tggcacatcg ctgcgttcgc ggcaccgcaa cattctggtc gctattggag taacagcgga
     3181 caaagggcgg cacgggcgct gaatggcttg gtcgcgaatg acccaaagcg aacgccgctc
     3241 gaaatcttca gacaaccgtt ccgaacaatt tgccgatacc ggcggtgaga gccatcgcca
     3301 acgcgcccca gaacgtaact cggaccatgg cgcgcaggat ctttgcaccg ccaacttttg
     3361 ccccgatcgc acccagaaga gcgagaaagc acaaagacgc agcgaacacg atcggaacaa
     3421 gtgcgcccgt gggcgatacg acaaccataa gcaagggcat cgccgcacct accgaaaacg
     3481 atgcggccga ggccagtgcg gcttgtacag gacgcgcggt ggtggttttc gaaatcccaa
     3541 gctcatcgcg ggcgtgggcg cctaaggcat ctttcgccat tagctgctgg gcaacttgcc
     3601 gcgcaagcac ctgatcgaca ccccgcttga cgtagatttc ggcaagctca tcaagctcaa
     3661 acgcaggatt ctcacttaac tccttgcgct cgcgcgcaag atctgcccgt tctgaatcgg
     3721 attgcgaact gacggacaca tactcgccgg cagccatcgc catggcgcca gccaccagcc
     3781 ccgcaacacc ggcgat
//
LOCUS       DASRKK010000680         8310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1338451, whole genome shotgun sequence.
ACCESSION   DASRKK010000680 DASRKK010000000
VERSION     DASRKK010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338451"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VFC15_16445"
     CDS             complement(<1..1658)
                     /locus_tag="VFC15_16445"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11798.1"
                     /translation="MAPRELLRWIEAFCNARTLQNRTAALIGLVGWMRKPGASIADLS
                     GLPGLVEYLDVNAGERVRFQAAFADLLSQMNCVSLFAEAGIPSDHSFVSEIGHRISAR
                     LLPSAREQSDAAKLLVTLYPTERNVKRFLASPPELFQRLVAVLSPPSDPEFASHEHRD
                     LLEALRLLASRVSALGLEPEVRARTSSPGVSDSPFYQIVSCIEDLIAASEPETARVCL
                     ARWQGAVHRCRTEMVRVHQDMETAGVSVELIFDLRKIAACVARMESIVDVLTAETGDA
                     RVSAVHGLLGHLMEGRLSDLSLSALLRENLNLIARKMVERTGHSGEHYIAHNRAEYWH
                     MWTAALGGGLLTVITAAIKLRVMDAHLPPFVEGFAAGTNYAVSFVLLQIFGLVLATKQ
                     PAATAATFAGIIRESRGVERENKLTEFVSRITSTQLAAAIGNVAAVCVGAIFFERLWL
                     FVFSKSYLAHETATHVYETLHPLASGTAFYAAVTGVILWMAALAGGWCENFAVYYRLP
                     EAIAQHPLGSKVGEVRLKKFSRVLDRNLGGWSTSIVLGYLLGFTP"
     gene            1845..2228
                     /locus_tag="VFC15_16450"
     CDS             1845..2228
                     /locus_tag="VFC15_16450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM11799.1"
                     /translation="MPTTFNGEAGAWRASAPVRATLVYDGEFDLTGAAAVSHIARSDS
                     MVDDKPEQALAEAREAVALTPNNVRAHISLGRALAKTGHPEEARYELRKALALANADR
                     AFYPLQIEAANDELRKLTGVTAGMP"
     gene            2300..3961
                     /locus_tag="VFC15_16455"
     CDS             2300..3961
                     /locus_tag="VFC15_16455"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HZM11800.1"
                     /translation="MLERRQFLLRSSLAACGLLLFRSPKSALAAGTGTACRIEVLLGE
                     QIGAISPNIYGHFTENLSGVIYDGIWVGKNSKVPNIDGIRTELVEAMRKIKPAVIRFP
                     GGCFADSYDWRDGIGPAEKRPHRTNFWDGAEGPSSPANHRYDPNQFGTDEFVHFCRLT
                     GGQPYLAANVRSLPAEEFDRWVEYCNSPAGSTTLADMRAAAGYREPFDVRYWGVGNES
                     WGCGGNFLPEEYAMEFRRYAAWVPGYGKPLSLIASGPNSDDLNWTRGFFDALARKGKD
                     QINSVYGFALHHYAWNLARGRTQDWDKAKGDALKFDPVDWYELLREGQKVEDLIDRHW
                     KLMGEIDTAHHVKLVVDEWGPWYKPGSEQTPGDALEQTPTMRDAVFSGMTLDIFNRNP
                     EKVAMANCAQLINCLNSLYLAHEDKFVVTPVGHVFEMYVPHQGGKALRAVFSAPAIQY
                     DRDGKPAEFWGLQGSASIRARTLVLTVVNPSVSESRETEISFSGAKAKSGSITTLSNS
                     DIHAHDSFQQRDVVVPRTSELKTASSALVVTFPPASVSTVQIELV"
     gene            complement(4045..5250)
                     /locus_tag="VFC15_16460"
     CDS             complement(4045..5250)
                     /locus_tag="VFC15_16460"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZM11801.1"
                     /translation="MVTSGKKGVIGILTGGGDVPGLNPAIRAVTIRALREGYSVIGLR
                     RGWASLVDTVRDAEYDNSDNYQILTEELVNRAGRTGGTFLHSSRTNPSKVSKASVPKH
                     LQDTYSADKNDLTPEVLKNLEWLGIENLIPIGGDDTLSYGVRLYQEGVKVVAIPKTMD
                     NDVPGTDYCIGFSTCVTRTIEMTNRLRTSAGSHERFMVLEVFGRYAGFTAMLPTMAGA
                     ANRCVIPEHKFDIDRLTELLSEDRRRNPSRYSVVLVSEGAMFEGGEMVFSQDAADAFG
                     HKKLGGIGDLVSEKIKELSPKYNNGKTIDVINQKLGYLVRCGDPDAIDSIAPMAYGNL
                     ALDLLLNRIHGRLVVLKNGRYDNMPIDAVTATKKVVNVKEHYNTDRLRPHYKSFEMKP
                     LFIMTSEAV"
     gene            5524..6504
                     /locus_tag="VFC15_16465"
     CDS             5524..6504
                     /locus_tag="VFC15_16465"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZM11802.1"
                     /translation="MATEPTTPALNEQDLRDLGFGAVVSRESQQRLLNRDGSFNVERS
                     GLGFWSSFSAYHAMLTIQWWQFFLAIAALYLVVNATFAAAYLACGPGALGSNAAGMEH
                     HTYLRAFFFSVQTLSTIGYGQVIPVGTAANALVTLESLVGLLGFAIVTGLLFSRFSRP
                     TAKMLFSRHALIAPYQKITAVEFRVANARSNELIEVSAKVLLSRFEQIDGIRTRRYYQ
                     LELERPAVTFLPLTWTVVHPITERSPLYLETPESMRNSQSELLVLLSGFDETFSATVQ
                     TRTSYIPDEVLWGYRFANAFVIGRAANNNKVTVDMRQFDKAESAQPALNR"
     gene            6766..7893
                     /locus_tag="VFC15_16470"
     CDS             6766..7893
                     /locus_tag="VFC15_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM11803.1"
                     /translation="MPYCERVPHRQPGRIKPGSGLLVILPVGLVLLLAACGRSKAESP
                     QGPPLVPVKVQRIGSSEIGNASEYVATIKSRNSATIMSDVEGWIFAIKVKSGDFVKKG
                     QSLMEIDPRRQAATVSNWESQRASKVANLQLAKVQLERAKGLSSSGVISKQDLDQAEA
                     TYDAANADVKSLDAQIHQQEVQLKYYSVFAPVDGIVGDIPVHVGDRVPNTTMLTTIDE
                     RRGLEVYLSIPSERAKELKIGAPVQILDGAGNVILTTRVDFISPQVDTASQSVLAKAP
                     VDQAADKLRSQQLVRARITWNTQPGITIPVVAVSRVSGQFFAFVAEQKDGKAVARQLP
                     LTLGEISGNDYTVLSGLKPGDEVVVSGGQNLADGMPITIQQ"
     gene            7926..>8310
                     /locus_tag="VFC15_16475"
     CDS             7926..>8310
                     /locus_tag="VFC15_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM11804.1"
                     /translation="MFADFFIKRPVFSTVTSLFIVIAGGVCIPFLPVAQFPQLAPPQV
                     SVNCFYTGANAQAVESAVTTPLEQSINGVEGMRYMTSSSGNDGSSSITVTFELTRSLD
                     IAAVDIQNRVSAVSGRLPNEVKTTGV"
BASE COUNT         1758 a         2386 c         2374 g         1792 t
ORIGIN      
        1 ggagtgaatc caagaagata tccgagcacg atgctggtgc tccagccgcc caaatttcta
       61 tccagtacgc gggaaaactt cttcagccga acttcgccga ctttcgaacc cagtggatgc
      121 tgcgcgatcg cctcagggag ccgatagtaa acggcgaagt tctcgcacca tccaccggcg
      181 agtgccgcca tccacaaaat tacgcctgtg accgcggcgt aaaaagcagt tcccgacgcc
      241 agtggatgca gggtttcgta gacgtgggtt gcggtctcat gcgccagata agatttcgaa
      301 aagacaaaca gccacagtcg ctcgaagaaa attgcgccca cgcatacggc ggcaacattg
      361 ccgatggccg ccgccaactg tgtgctggtg atgcgtgaga cgaattccgt aagtttgttt
      421 tcgcgctcca cgccccggct ctcgcgaata atcccggcaa aggtcgccgc ggtcgccgcc
      481 ggctgcttgg tggcaagaac gagtccgaaa atctgaagca gcacgaagct tacggcatag
      541 ttcgttccgg cggcgaatcc ctccacgaac ggtggcaaat gggcgtccat gacccgcagt
      601 ttaatggcgg cggttattac ggtcagcagt ccgccgccca aagcggccgt ccacatgtgc
      661 caatattctg ctcgattgtg cgctatgtag tgctcgccac tatggccggt gcgttcgacc
      721 atcttcctgg caatcaagtt gaggttctcg cgcaacaaag cggacaagct aaggtccgag
      781 agacggccct ccatgagatg gccgagcaat ccatgtaccg ccgaaactcg ggcgtcgccg
      841 gtctccgcgg ttaggacatc aacgatcgat tccatcctcg cgacgcaggc agcaatcttt
      901 cgcaggtcga agatcaattc gacactgact cccgcagttt ccatgtcctg atggactcgc
      961 accatttccg tgcggcaacg atggaccgcg ccttgccagc gtgccagaca gacgcgcgct
     1021 gtctcgggtt cggatgcggc aatgagatcc tcgatacacg aaacgatctg gtagaacggc
     1081 gaatcggaaa cgccaggcga tgaggtgcgg gccctgactt ccggctccag ccctaaagcg
     1141 ctcacccgcg aagcgagcag gcggagcgct tccaacaggt cccgatgctc atggctggcg
     1201 aactcgggat cgcttggtgg gctcagaacc gcgaccagcc gctggaacaa ttctggtggg
     1261 ctggccaaaa accgtttcac gttccgctcg gttggatata acgtcaccaa cagcttcgca
     1321 gcatcggact gttcgcgcgc cgacggcagc agacgggcag aaatgcgatg accgatctcc
     1381 gatacgaagc tgtggtccga aggaattccc gcctcggcga acagcgagac gcagttcatt
     1441 tgggagagca aatcggcgaa cgcagcctga aagcggactc tctctccggc gttgacgtct
     1501 agatactcaa ccagccctgg caagccggat aggtctgcaa tcgaggcccc aggttttctc
     1561 atccagccga cgagtccgat taacgctgcg gtgcggtttt ggagggtgcg agcattgcag
     1621 aatgcttcta tccaacgcag cagttcgcgt ggcgccatcc gcgaaggcac cgggccggcc
     1681 ccagacgttt ctgaagacgc gtatgctggt gtgcccgcga tttctggctg attcactggt
     1741 tcggtcgaca cagtcccatt gtccgataga ccagccacga tgccaagtgc ccggcatggc
     1801 tggtcgagtc acacaggtta gagtcactga tcgcgaatct gacgatgcca accacattca
     1861 acggcgaggc aggagcatgg cgggcctcgg cgcctgtccg cgcgacgctg gtctatgatg
     1921 gcgagttcga tctaacgggt gcagcggcag tcagccatat cgcaaggtcc gacagcatgg
     1981 tggatgacaa acctgagcaa gcgttggcgg aagccaggga ggcggtggca ctcacgccga
     2041 ataacgtgag ggcgcatatc tccctgggac gcgcgcttgc gaagactggc catcctgagg
     2101 aagcgcggta cgaacttcgg aaagctcttg cgctggcaaa tgcggaccgc gcgttttatc
     2161 cgctgcagat cgaagccgca aacgacgaac taagaaagtt gacgggcgtt actgccggga
     2221 tgccatagct ctcgttggaa ttgaatcgac gcgccgcggt cattgcggtg atcactgaca
     2281 agttagggag aaagacatca tgttagaacg ccggcaattt cttcttcgga gttccctcgc
     2341 ggcctgcggc ttattgttgt tccgcagccc gaaatcggcg ttggctgccg gcaccggcac
     2401 ggcttgccga attgaggtcc tcctcggcga gcaaataggc gccatctctc ccaacatcta
     2461 cggtcacttc accgagaacc tcagcggcgt catttacgac ggtatctggg tggggaagaa
     2521 ctcaaaggtt ccaaacatcg atggcatccg caccgagctc gtcgaggcta tgcgcaagat
     2581 caagcctgcg gtcattcgat tccccggagg atgctttgcc gacagctacg actggcgcga
     2641 cggtattggt cctgcggaga agcgcccgca ccgaacaaac ttctgggacg gtgctgaagg
     2701 cccgagttca cccgctaacc atcgatatga ccccaaccag tttgggaccg acgagttcgt
     2761 gcatttctgc cgattgactg ggggccaacc ctaccttgcg gctaatgtgc gcagccttcc
     2821 agcggaggaa ttcgatcgtt gggtggagta ctgcaactct cctgctggga gtacgacgtt
     2881 ggctgacatg cgcgcggccg ctggatatcg cgagccgttc gacgtccgct attggggagt
     2941 gggcaacgag tcgtggggct gtggcggaaa cttccttcct gaggaatatg ccatggagtt
     3001 ccgtcgctac gccgcctggg tcccaggcta tggcaagccc ctatcgctga tcgcctcggg
     3061 accgaattca gacgatctga actggacccg tggcttcttc gacgcgctgg cgcgcaaagg
     3121 gaaagaccag atcaacagtg tctatggctt tgccctgcat cactatgcct ggaacctggc
     3181 gcggggcagg acgcaagact gggacaaggc gaaaggcgac gccctgaaat ttgatccggt
     3241 ggactggtac gagttattgc gcgaaggtca gaaggtcgaa gacctgatcg accgtcattg
     3301 gaagctcatg ggagaaattg acaccgcgca ccatgtcaag ctggtggtcg atgaatgggg
     3361 cccgtggtac aagcctggca gcgaacagac tcccggcgac gcgctggaac agacgccgac
     3421 gatgcgtgat gcggtattca gcggcatgac tctcgatatc ttcaaccgca accccgaaaa
     3481 ggttgcgatg gccaattgcg ctcagctcat caattgcctc aacagcttgt acttggccca
     3541 cgaagacaag ttcgtggtca cacctgtcgg tcacgtgttc gagatgtatg ttccgcacca
     3601 gggtggaaag gcgcttcgtg cggttttcag cgctccagca attcaatatg atcgggacgg
     3661 taaaccggcg gagttctggg gactgcaagg atccgcatcg atccgcgcaa gaaccttggt
     3721 gctgacggtc gtcaacccca gtgttagcga gtcccgggaa actgagattt cattttcggg
     3781 cgccaaggcg aagtctggca gtattacgac tttgtccaat tcggacattc atgctcacga
     3841 ctcattccag caacgggacg tcgtggtgcc acgaaccagt gagttgaaga ctgcgagttc
     3901 agcactggtt gtgacctttc cgccagcatc ggtcagcacc gtgcagatcg aactggtctg
     3961 aatcgcggaa tccgcctagg cgttctgcaa ctaaaaaggg gatcgcttga cgcaacccgc
     4021 cattatccga cacagattgg aaagctatac cgcttcgctg gtcataataa ataggggctt
     4081 catttcgaag ctcttgtagt gcggacgcaa gcggtcggtg ttgtagtgct ccttcacatt
     4141 tacgaccttc ttggtcgcgg tcacggcgtc gattggcatg ttgtcatacc ggccattttt
     4201 caataccacc aggcgcccat gaattcggtt taatagcagg tctagcgcga gattgccata
     4261 ggccattggc gcgatggagt cgatggcatc gggatctccg caacgaacca ggtagcccag
     4321 cttctgatta attacgtcaa tggtttttcc attgttgtat ttgggcgaca gttccttgat
     4381 tttctccgac actaggtcgc caatgccccc aagtttcttg tggccgaagg cgtcggcggc
     4441 gtcttgcgaa aaaaccatct cgccaccctc aaacattgcg ccttccgata ccagcacgac
     4501 tgaatagcgg ctggggttgc ggcggcgatc ttcgctcagc agttcggtca ggcgatcgat
     4561 gtcaaacttg tgctcgggta ttacgcagcg attcgcggct ccggccatgg tcggcaacat
     4621 cgccgtgaag ccggcgtaac ggccgaacac ttcgagaacc atgaagcgtt cgtgggaccc
     4681 ggccgacgtt cgcagccgat tcgtcatctc gatggtgcgg gtgacgcagg tgctgaatcc
     4741 gatgcaatag tctgtccccg ggacatcgtt gtccatggtt ttcggtatgg ccacgacctt
     4801 cacgccctcc tgatagaggc gcacgccgta gctcaaggtg tcatcgccac cgatggggat
     4861 caagttctcg atgccgagcc actccaggtt cttcagaact tcgggagtca gatcgttctt
     4921 gtcagccgag taggtgtctt gcaggtgctt ggggacgctg gccttgctca ctttgctggg
     4981 attggtgcga gaactgtgaa ggaacgtgcc gccggtgcgc ccagcgcgat tgaccaactc
     5041 ctcggtgaga atctggtaat tatcgctgtt gtcatattcg gcgtcgcgca cggtgtcgac
     5101 caggcttgcc caccctcggc gaagtccaat cacactgtag ccttcgcgga gcgccctgat
     5161 ggttactgct cgaattgccg ggttcaatcc cggtacgtcg ccgccgcccg ttaaaattcc
     5221 gatgacgccc tttttgccgc tagtaaccat cgatgatccc taccctccga atggtgaaaa
     5281 gactattaaa catgaatctt ctgatgcgcc gctgtgacat tccgcacagt cgcccgaccc
     5341 gtattttcgg atggcagtgc caacctgttg aattcgtcga cgcatcccag ttcgttcggc
     5401 agaaatctca ttctgggcgg cgggaatcat gcgctaccct gggtcgcaag aatcttcctg
     5461 cgccgcgaaa ctcgatatac tctcagaacg ccggcggccc gacgccgttg gacagaaatt
     5521 tccatggcga cagaaccgac aacaccggca ttgaacgaac aagatctgcg cgaccttggt
     5581 tttggcgcgg tggtgtcgcg cgagagccaa cagcgcctgt tgaaccgcga cggcagcttt
     5641 aatgtcgagc gctccggact cggattttgg tcgtccttca gtgcctacca cgccatgctc
     5701 acgattcagt ggtggcagtt cttcctggcc attgccgcac tgtatctggt cgtcaatgca
     5761 accttcgcgg ccgcatatct tgcctgcgga cctggggccc tgggaagcaa cgccgcggga
     5821 atggaacatc atacttacct gcgcgcgttc tttttcagcg tgcaaaccct gtcgaccatt
     5881 ggctatggtc aagtcattcc tgtcggcaca gccgccaacg ccctggtcac cctggaatcg
     5941 ttggttgggc tgctgggatt cgccatcgta accggcctat tgttctcccg gttttcccga
     6001 ccgactgcca agatgctgtt cagccggcac gcgttgatcg ccccgtacca gaaaatcacg
     6061 gccgtcgaat ttcgtgttgc caatgcccgc tctaatgaac tgattgaagt ctccgcgaaa
     6121 gtgctgctca gccggtttga acagattgac ggaatacgga cgcgccgcta ctaccaactt
     6181 gaactcgaac gcccagctgt aacgtttctg cccctgacct ggaccgtggt gcatcccatt
     6241 actgagcgca gcccgttgta tctggagact ccggaatcga tgcgcaactc gcagtcggaa
     6301 cttctagtgc tgctcagcgg attcgacgag acattctccg ccactgtcca gacgcggacc
     6361 tcgtacatcc ccgatgaagt tttgtggggt taccggtttg cgaacgcctt cgtgatcggc
     6421 cgcgctgcca acaataataa agtcacggtg gatatgcggc agtttgacaa ggcggaatcg
     6481 gcgcaacctg cactcaatcg atgagtgtcg tcaccgtgtt cggggatttg aggtggatgc
     6541 cgaacattca agtgcctcac tgacgaccat taatctgtat ctaacgacga tcgtgcgcgg
     6601 gcactcacaa cctcggtcaa gcggttcatc ccagtcattc cgtcggacat aattgcatcc
     6661 tttgcctcgg gatacaatct agtaacccta acaaaaccgt tatttgtacc catgatgttg
     6721 cagccggaga agttcttatt gcatacccca ggaaccggaa gttttatgcc atattgtgag
     6781 cgcgtaccgc accgccagcc cggcaggatc aaaccgggta gcgggctgtt agtcattctc
     6841 ccagtcgggt tggtgttgct gctggctgcc tgtggtcgca gcaaggccga gagtcctcaa
     6901 ggtcctcctt tggtgccagt gaaagtccag cgcattggat cgtcggagat cggcaacgcc
     6961 tcggagtatg tcgccacaat taaatcccgc aattccgcga ccatcatgtc cgatgtggag
     7021 ggctggatct ttgccatcaa agtgaagtcc ggcgattttg tgaaaaaggg ccagtcgctg
     7081 atggaaatcg atccccgtcg tcaggccgca acggtttcca attgggagtc gcaacgggcc
     7141 tccaaggtgg cgaatctgca actggcgaag gtccaactcg aacgagccaa aggtctgtcc
     7201 tcatcaggcg taatcagcaa gcaggacctc gaccaggcgg aggcgactta cgacgcggcc
     7261 aacgcggacg tgaagtctct cgacgcccag atacatcagc aggaggtaca gctcaagtat
     7321 tacagtgttt tcgctccggt ggacggtatc gttggtgaca ttccagtgca tgttggcgac
     7381 cgggtcccca ataccaccat gctgacgacc atcgatgaac gccgggggct tgaggtgtat
     7441 ctttccatcc cttcggagcg cgcgaaggag ctgaagatcg gcgctcccgt gcagattctg
     7501 gacggcgccg gtaacgtgat cctgaccaca cgggtagact tcatctctcc gcaagtcgac
     7561 accgcatcac agtccgtgtt ggccaaggcc ccggttgacc aggcggctga caagctgcgt
     7621 tcccagcaat tggttcgtgc ccgaatcacc tggaacacgc aacccggcat caccatccct
     7681 gtggtggcgg tttcccgcgt aagtggccag ttctttgcgt ttgtggccga acagaaagac
     7741 ggcaaagctg tcgcccgtca acttcctttg acgttggggg agatatcggg caacgactat
     7801 accgtattga gtggcttaaa acctggcgat gaggtcgttg tttcgggcgg gcagaatctt
     7861 gccgacggaa tgccaattac tattcagcag tagctcacgg cttctcagaa gaaggggcat
     7921 tcgtcttgtt tgctgatttc tttatcaagc gtccagtctt ctcgactgtg acctctttat
     7981 ttatcgtgat cgccggcggg gtctgcattc cttttctgcc cgtcgctcaa tttccacaac
     8041 tagctccccc acaagtttca gtcaactgct tttataccgg cgcgaatgcg caggccgtgg
     8101 aatcggccgt gaccacacct ctggagcagt ccatcaatgg cgtagaaggc atgcgctaca
     8161 tgacgtcgag cagcggcaac gatggcagca gctcgatcac cgtcaccttt gagttgactc
     8221 gaagcctcga tatcgctgcg gtcgacatac agaaccgcgt ctcggcggtc tcagggcgtc
     8281 ttccgaatga agtgaagaca actggtgtgg
//
LOCUS       DASRKK010000681         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_752724, whole genome shotgun sequence.
ACCESSION   DASRKK010000681 DASRKK010000000
VERSION     DASRKK010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752724"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1227)
                     /locus_tag="VFC15_16480"
     CDS             complement(28..1227)
                     /locus_tag="VFC15_16480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZM11805.1"
                     /translation="MSFRFQVEARDGAARAGRFFTPHGEVQTPVFMPVGTLATVKGVP
                     QDTLEELGVQILLNNTYHLYLRPGVEMVRRLGGVQKFMAWDHPILTDSGGFQVFSLSD
                     LRKVTEHGVAFRSHLDGSSHFFSPESAIAAEIGLGADIIMAFDECTEHPAEYARTQAS
                     MEMTLRWAARSQKYFEDHKNEVPWFAERDGETPGSPTRADFAHVGVGETQTLFGIVQG
                     GMFPDLRRESAQRTVELDFPGYAIGGLSVGEPRPMTYEMVDVAMPFLPEDKPRYLMGV
                     GTPEEIVHYVAMGVDMMDCVLPTRAARHGLLFTSEGRLTIKNARYATDDGPLDPNCPC
                     QVCSRYSRAYLRHLYASNELLAQVLNTVHNLAYYLDTMRKVRQAILLGEFAIFLSGVR
                     PPHLSVP"
     gene            1589..2209
                     /locus_tag="VFC15_16485"
     CDS             1589..2209
                     /locus_tag="VFC15_16485"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZM11806.1"
                     /translation="MRAEDLVLAGRYVRLEPLDHRHVDGLVAASATDPTLYQWSPVPQ
                     GKAEATSYVNTALAWRDAGSAVPFAIIRVDDGVVIGSTRFFNLERWLWPQDHARHGLL
                     DACEIGYTWLTRSAIRTAANTEAKLLMLTHAFETWKGLRVCFHTDVRNQRSRAALERI
                     GGKLEGILRAHRMAADYIARDSARYSIVVAEWPDVKRRLSQLLDQT"
     gene            complement(2550..>2954)
                     /locus_tag="VFC15_16490"
     CDS             complement(2550..>2954)
                     /locus_tag="VFC15_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM11807.1"
                     /translation="DGFRVEVGALQNTLESAPHLQLMLSRYAAVRSIQVAQTAACNRL
                     HDIEQRLARWLLMAQDRVDSGSLPITHDFLATMLGTDRPTVSLAAGVLQRKKLIEYTR
                     GAVKIVHRKKLEDSACECYGVIQQYDGELGLK"
BASE COUNT          614 a          878 c          872 g          590 t
ORIGIN      
        1 tttgccgggg acccgaaccg aaggctgcta aggaacactc aaatgcggtg gccggacgcc
       61 agaaagaaaa atcgcgaact ccccaagtaa tatagcttgc cgcaccttac gcatggtgtc
      121 aaggtaatat gccagattgt ggacggtgtt cagcacctga gcgagcagtt cgttggaggc
      181 atacagatgc cgcaggtaag cccgtgagta gcgcgagcag acctgacaag ggcagttcgg
      241 gtcaagcgga ccatcgtccg tggcatagcg cgcgttcttg atggtaaggc ggccttcgga
      301 ggtgaagagc agcccgtggc gggccgcccg cgtgggcagc acgcagtcca tcatgtccac
      361 gcccatggcc acgtagtgca caatttcttc cggggtgcct actcccatca ggtagcgcgg
      421 cttgtcttcc ggcaggaatg gcatggccac gtccaccatc tcgtaggtca tggggcgcgg
      481 ttcgcccacg ctcaagccgc caatcgcgta gccggggaaa tcaagctcca cggttcgctg
      541 tgccgattcg cgacgcagat cgggaaacat gccaccctgc acgattccga ataacgtctg
      601 tgtctcaccc actccaacgt gcgcaaaatc ggcgcgcgtt ggggaccccg gagtctcacc
      661 gtcgcgctcg gcaaaccacg gaacctcatt cttgtgatct tcgaaatact tctggctgcg
      721 cgccgcccaa cgcaacgtca tctccatgga cgcctgagtg cgtgcgtact ccgccggatg
      781 ttcggtgcac tcgtcgaacg ccatgataat gtcggcgccg agtccgatct cggcggcgat
      841 ggcgctctcc ggactgaaga aatgcgagga gccgtcgaga tgcgagcgga acgccacgcc
      901 gtgttccgtg accttgcgca gatcgctgag gctgaaaacc tgaaagccgc cggaatcggt
      961 gaggatggga tggtcccacg ccatgaactt ctgtaccccg ccgaggcggc ggaccatctc
     1021 gacaccgggg cgcagataaa gatggtaagt gttgttcagc aggatctgga cgccgagctc
     1081 ttccagcgtg tcctgcggta cgcccttgac tgtcgccagc gttcccaccg gcataaagac
     1141 tggggtctgg acttcaccat gcggagtgaa gaagcgtccg gcacgggcgg cgccatcgcg
     1201 agcttcaacc tgaaagcgga aggacattgc agacgattgt aatcggatca ccacagaggc
     1261 acagagacac agagaaatca gaagcgatac tcaatgctgg gttagttcca ccacggagga
     1321 cacggagcgc acggagacga tctagagata acccgaaggc cgaacccttc ccccgcaagc
     1381 ctaagaatcg gtcccgattt ctctgggcct ctgtggtgat ccgattacag tcgtctgatt
     1441 gtcctttcga tttcggctca atgcctgatc ttctccgtgt gctccgtgtc ctccgtggtg
     1501 caaaatcaag ccgcagacga tctctgctgg tgtgccggct tcgtaagttc cattgcggct
     1561 gtccggtggc tcattgcata cttggtgcgt gagagcagaa gaccttgtgc tggctggcag
     1621 gtacgttcgc ctcgaaccgc ttgaccaccg ccatgtcgac gggttggttg ccgcctcggc
     1681 gacggacccc actctctacc aatggagccc tgttccgcag ggcaaggccg aggccaccag
     1741 ctacgtcaat accgccctgg cctggcgaga cgccggcagt gcggtgccat tcgcgatcat
     1801 ccgcgtggat gatggtgtcg tcatcgggtc gacccgcttc tttaacctgg agcgatggtt
     1861 gtggccgcag gaccacgcgc ggcacggcct tctcgacgcc tgcgagatcg gttacacgtg
     1921 gctgacacgc tcggccatcc ggactgcggc caacaccgag gccaagctgc tgatgctgac
     1981 gcatgccttt gaaacgtgga aggggctgcg ggtctgcttt cacaccgacg tacgtaacca
     2041 gcgttcacgc gccgcgctcg aacgcatcgg cgggaagtta gaggggattc tccgggccca
     2101 tcgcatggcc gctgactaca tcgctcgcga ttcggcgcgc tactcgattg tggtggcgga
     2161 gtggcctgat gtgaagcgaa ggctgagtca gctcctggat caaacgtgaa cgacgttcgg
     2221 gtggtgtttg gcgacctatg taacgtcgcc gagcgtggtg aaccgagaga tcggaaacag
     2281 gttcgccatg actaacgtgt agcagcctga actcgacctt acggacagcg ccgacgagtc
     2341 ggcatttgtc gggcctcgaa cggcattccg gacggagttc ccaacttccg ccttgctgac
     2401 gacccgctca gaaccatcct gatttggtgc gagtgagcaa cgagcatgca tggccatttc
     2461 gattgaacgg tggttggtgt aaacgggatt ttatgaacaa caacgcttcc aagtcggcaa
     2521 ttaagcacag agcgagtctt cacccggact tatttcaagc caagctcgcc atcgtattgt
     2581 tggatgaccc cgtagcactc gcaggcggag tcttcaagtt tcttgcggtg cacgatcttc
     2641 actgcaccac gggtgtactc aatgagcttc ttcctctgca aaacaccagc cgccaagctc
     2701 acggtaggcc gatctgtccc tagcatggtt gcgagaaaat catgggtaat gggcagcgac
     2761 cctgaatcca ctctatcctg agccattagc aaccaccgcg ccaggcgctg ctcaatatcg
     2821 tgcagccgat tacacgcggc tgtttgtgca acctgtatac tccgaaccgc ggcatagcgg
     2881 ctcaacatca attgcaggtg gggcgcggat tccaaagtgt tttgcaaagc tccgacctct
     2941 acccgaaacc catc
//
LOCUS       DASRKK010000682         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_555991, whole genome shotgun sequence.
ACCESSION   DASRKK010000682 DASRKK010000000
VERSION     DASRKK010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555991"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="VFC15_16495"
     CDS             complement(<1..1656)
                     /locus_tag="VFC15_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11808.1"
                     /translation="MQNLNTIHDYLRTHSEEIGERILSSYPALHGADETPAPLLSRML
                     RAPYPAQTLAIMAVSKRWLFARNANVVAECGSGKTLIALGSMLVHSAGRPYSGLVMAP
                     PHLVEKWARETFLTLPQVRVFLIDDMRNGGDPTQPHGINEVRLRRGEIVREGLHTSLA
                     ELRRLGRKGWRSLCPGTTIFCIGREKAKLSYFWKHCYGKSRSGRYLGSLTNPDTGCPV
                     ETDGVRLSALDFEKKRLHQIVEDSKAGKTKYSALWQADCNKIARMAPAEYMGRYMPEW
                     WDYAIADEIHQLAGDTAQGNALGVLNKTARRFLGLTGTLLSGYADDLFNTLFRTDAKR
                     MIEDGYEWGSAGRERFTRDFGVIETIERITVEDNACSRKTKKTVTIKRKPGASPLLFG
                     KYLMDHCAFIGLEDISDGLPSYREEVVSVGMQEPLKTAYEELEEEITACLKEHRGNSS
                     VASTMLNALLAWPDHPYGFGTLYGSEFDPETKCRESFVIAETADLDKNETYAKERALI
                     EEVKAQLSRGRKCQVFAVYTNKHDVTARLEQLFRAEGIRASILR"
     gene            1729..1965
                     /locus_tag="VFC15_16500"
     CDS             1729..1965
                     /locus_tag="VFC15_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11809.1"
                     /translation="MKLEVDCYSGRAADERPVRFRLDGHEYMVEEVLDQWYGPSDVFY
                     KVRADDGNLYILRRVTSTPDGLWHLESFRQLPRH"
BASE COUNT          410 a          618 c          625 g          494 t
ORIGIN      
        1 cctcaggatc gacgctcgta ttccctcagc gcgaaataac tgttccaacc gtgccgtaac
       61 gtcgtgcttg ttggtgtaga cggcaaatac ctggcatttg cggccgcgcg agagctgggc
      121 tttcacctct tcgatcaatg ctcgctcttt ggcataagtc tcgttcttgt ccaggtcagc
      181 cgtttcggca atgacaaacg actcgcggca cttggtttcc gggtcgaatt cagaaccgta
      241 caaagtgccg aagccatacg gatggtccgg ccatgcgagc agtgcgttca gcatggtgct
      301 cgccacgctg ctgtttccgc gatgctcctt caggcaggca gtgatctcct cttcgagttc
      361 ttcgtaggcc gtcttcagtg gttcctgcat gcccactgat acgacttcct cccgatagct
      421 tggcaaaccg tccgagatgt cctccaggcc gatgaacgcg cagtgatcca tcagatactt
      481 gccgaacagt agcggtgagg ccccaggctt gcgcttgatc gtgactgtct tcttggtttt
      541 ccgggagcag gcgttgtctt cgactgtgat gcgctcaatg gtctcgatca cgccgaagtc
      601 ccgggtaaag cgttcccggc ctgcggagcc ccattcatag ccgtcctcaa tcatgcgctt
      661 ggcgtcggtg cggaacagcg tgttgaaaag atcgtccgcg tagccggaga gtaaagttcc
      721 tgtcagtcca aggaaccttc gtgccgtttt gttgagcacg ccgagagcat tgccttgggc
      781 cgtgtcgcca gccaactggt ggatttcgtc cgcgatggcg tagtcccacc attccggcat
      841 gtagcggccc atgtattccg ccggagccat gcgggcgatc ttgttgcagt ccgcctgcca
      901 cagcgcggaa tacttggtct tccctgcctt cgagtcctcc acgatctggt gcagacgttt
      961 cttctcgaag tcgagtgcgc tcagccgcac gccatccgtc tcgaccggac agccggtatc
     1021 gggattggta agcgagccaa gataccgtcc ggagcggctt ttgccgtaac agtgcttcca
     1081 gaaataggag agcttggctt tctctcggcc aatacagaag atcgtggttc cgggacagag
     1141 acttcgccat cctttccgcc ccaggcgccg caactctgct agcgaggtgt gcaagccctc
     1201 gcggacgatt tcgccgcgcc gcagccggac ctcgttgatg ccatgcggtt gtgtcggatc
     1261 gccaccattg cgcatgtcgt cgatgagaaa cacccgcact tgcggcagtg ttaagaaggt
     1321 ctcccgcgcc catttctcga ccaagtgcgg tggcgccatc accaggcccg aatatggcct
     1381 gccggcgcta tgaaccaaca tgcttccgag agcaatgagt gtcttgccag agccgcactc
     1441 cgctacgaca ttggcgttgc gcgcaaaaag ccaacgcttg ctcacagcca tgatcgccag
     1501 ggtttgtgcc ggataaggcg cgcgcagcat ccgagagaga agtggagccg gcgtttcgtc
     1561 ggcgccgtgc agggcggggt acgacgacaa aatgcgttcg ccgatctcct cgctgtgagt
     1621 tcggagataa tcatggatag tgttgaggtt ttgcatggat ttctccggga aggaggtgta
     1681 aaggcgcgcg gatggcgcgc ttaacgccta tgcgtcagaa tgaaggagat gaagctggaa
     1741 gtggattgct attccggtcg ggcggcggac gaaaggccgg ttcgctttcg gctggacggg
     1801 cacgagtaca tggtcgagga agtactggac cagtggtacg gcccatcaga tgtcttttac
     1861 aaagtccgtg cagatgacgg caatctctac atcctgcgcc gggtgacatc cacgcctgat
     1921 ggcctttggc atttggagtc atttcggcaa ctgccacgcc attgaaggct agcggtcaat
     1981 ctcttgtcct tgaatcttcc ttgtccccgg acgaacaagg ctctaagaat ggaaacaggc
     2041 gtctagccac agcttcagcg tgagatcgtg ccggctctca ttggcgagat atggtcgcaa
     2101 ttcctgttcg taccgctgtc tgagcacggt ttcatccaac ttctctg
//
LOCUS       DASRKK010000683         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1240499, whole genome shotgun sequence.
ACCESSION   DASRKK010000683 DASRKK010000000
VERSION     DASRKK010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240499"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1196)
                     /gene="istB"
                     /locus_tag="VFC15_16505"
     CDS             complement(411..1196)
                     /gene="istB"
                     /locus_tag="VFC15_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM11810.1"
                     /translation="MSAAPQIRTALTENLKDLHLPAMRECFEQAAHQAEKETLSYEQY
                     LLQLTERECESRRRNRIAALLRESGLPREKTMANFDLKRLPVKAGRQLKALVDGSFLD
                     RKENLLLFGNPGAGKSHLLCALAHELIAQGRRMKYTSCAMLVQDLLVAKRDLRLSKEI
                     KKLARHDGLIIDEMGYVQQSREEMEVLFTLLSERYERGSVLLTSNLPFSKWEGIFKDP
                     MTTAAAIDRLVHHSVIVELNIPSYRMEEAKKNRDPPQAGSEVN"
     gene            complement(1193..2638)
                     /gene="istA"
                     /locus_tag="VFC15_16510"
     CDS             complement(1193..2638)
                     /gene="istA"
                     /locus_tag="VFC15_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM11811.1"
                     /translation="MKTEENQEIAASKAGMDAKTARKYRRHGGVPSELPRAVRGRTRP
                     DPFLDVWGEVQKLFEDNAGLEAKTVFEFLQRQNPGRFQDGQVRTLQRRVKVWRATEGP
                     AKEVFFAQQHPPGRLGASDFTHMEELGVTIQGQSFPHLIYHFVLTYSNWEAATVCFSE
                     SFESLSEGLQNALWALGRVPHRHRTDRLSTAVNNMSDPAEFTDRYKGLMSCYGLESEK
                     TQAGHGNENGDVEQRHHRFKRAVAQELMLRGSVDFGSVDEYRRFLAAMLERLNAGRRQ
                     RLGEEMAVMRELPERRMESARRDRVKVDSGSLIYVDRNVYSVPSRLIGEQVEARLYME
                     HVEVWYGQQKIAEMPRLRGRQKHRVDYRHIIDWLVRKPGAFEHYRYRDELFPTSRFRM
                     TFDLLEEQLGRDQGSKEYLKILELAARDSEVGVDAALLVLLGAGDEPISAAGIEAMLG
                     AEHSMTVRDVHVAEVDLRVFDQLFSAQEVLQ"
BASE COUNT          605 a         1021 c          823 g          647 t
ORIGIN      
        1 ctccaaactg cctaacttag ctgtatggaa gccgcatggg aaaatcccat cggcactgtc
       61 ttttgcgaga cgtgtgccga acggggacgg ccaaatgagc ccgtctacaa gatggggatg
      121 tgcgaattct gctacaaggg cttgcgacat cccaaagcaa ccagagagca actggccaaa
      181 gaggggggca tgaaagaccc accatgagca catcgacgta gatttagtca tttttgtctg
      241 gcgtcaacta cttgttccgg tcaacgacaa ccataaatcc cgatagtgtt ggaacgtctt
      301 tatctcctcc cgctgctgag atggtgttgt tgctggcagg gccggagcgt agccccgaag
      361 gggcggagcg gaggccctgc cagcaacagc ggacgccgct ctccgccccg tcagttgact
      421 tcgctgcctg cctgcggcgg gtcccgattc tttttggcct cctccatgcg gtagctgggg
      481 atgttcagtt ccacgatgac cgagtgatgc accagccggt cgatggctgc cgccgtggtc
      541 atgggatctt tgaaaatccc ctcccacttg gagaacggaa gattgctggt cagcagtacg
      601 ctgccgcgtt cgtatcgttc ggagagcagg gtgaacagca cctccatctc ttcgcgactt
      661 tgctggacgt agcccatttc atcgatgatc aagccgtcat ggcgggccag cttcttgatc
      721 tctttgctca gccgcaagtc gcgcttggcc accagcagat cctgcaccag catggcgcag
      781 ctggtgtact tcatcctgcg tccttgcgcg atcagttcat gggccagggc gcatagcaag
      841 tggctcttgc ccgcgcccgg attcccgaac agaagcaagt tctccttccg atcgaggaag
      901 ctgccatcca ccagcgcctt cagttgtcgt ccggctttga cgggtaaacg cttcaggtcg
      961 aagttggcca tcgtcttctc tcgcggcagt cccgactccc gtagcagtgc ggcgatgcgg
     1021 ttgcgccgtc gagactcgca ctcccgctcc gtcaactgca acagatattg ctcgtagctg
     1081 agagtctctt tctccgcctg gtgcgcggcc tgctcgaagc actcgcgcat agccggtaga
     1141 tgcagatctt tcagattctc cgtcaacgcc gttctgatct gaggggccgc gctcattgca
     1201 gcacctcctg cgcactgaac aactggtcga agacacgcag atccacctcc gccacatgca
     1261 catcgcgaac ggtcatgctg tgctcggctc ccagcatggc ctcgattccc gccgcgctga
     1321 tcggctcatc gcctgctccc aacagaacca gcagagccgc atcgactcca acctcactgt
     1381 cccgagcagc caactccagg atcttcaggt attccttgct gccctgatca cggccaagct
     1441 gctcctccag caggtcgaag gtcatgcgga accggctagt ggggaacaac tcgtcgcgat
     1501 agcggtagtg ctcgaaggca cccggcttgc gcaccagcca gtcgatgatg tgccggtaat
     1561 ccacccgatg cttctgccgc ccgcgtaagc gtggcatctc ggcgatcttc tgctgcccgt
     1621 accagacctc cacgtgctcc atgtacagcc gtgcctccac ctgctcgccg atcaaccggc
     1681 tcggcaccga gtacacgttg cggtccacat agatcaggct gcccgagtcc accttgaccc
     1741 gatcacgcct ggccgattcc atgcgccgct caggcaactc ccgcatcacc gccatctctt
     1801 cgccaagccg ctgcctgcgc ccggcgttca accgctccaa catcgctgcc aggaaacgcc
     1861 ggtactcgtc cacgctgccg aaatccacgc tgccgcgcaa catcaactcc tgcgccacag
     1921 cccgcttgaa gcgatggtgc cgctgctcca catcgccgtt ctcgttgcca tgcccggcct
     1981 gcgtcttctc gctctccagt ccatagcaac tcatcagacc cttgtagcga tccgtgaact
     2041 cagccggatc gctcatgttg ttgaccgccg tcgaaagccg gtcggtgcga tggcgatgcg
     2101 gcactctacc cagcgcccac agcgcgttct gcaacccttc gctcaggctc tcgaagctct
     2161 ctgaaaagca cactgtggct gcctcccagt tcgagtacgt cagcacgaag tgatagatca
     2221 ggtgcggaaa actctgcccc tggatggtca cgcccaactc ttccatgtgc gtgaagtcgg
     2281 acgctcccag ccgacccgga ggatgctgtt gcgcgaagaa cacctctttg gccggaccct
     2341 cggttgcacg ccacaccttt acgcgccgct gcaatgtgcg cacttgcccg tcctgaaagc
     2401 gacccggatt ctgccgctgc aagaactcga agaccgtctt ggcctcaagc ccggcgttgt
     2461 cttcaaacaa cttctgcacc tcaccccaga catccagaaa cggatctggt cgggtccgtc
     2521 cccgcaccgc cctcggtaac tcgctcggca ccccgccatg acggcgatac ttccgcgccg
     2581 tcttcgcgtc catccccgcc ttggacgccg caatctcttg gttttcctct gtctttatca
     2641 atgcaaacaa cctccgaacc tgcctgtctg tgaccaatcc gcctccgctc cgatgaaccg
     2701 gaatacgctt ggcctaattg tcgttggatc gggaattttg tttgtcgtcg ttcggtactt
     2761 ctaattgtcg cccgtcaggt gcccgagttg caagaaactg ctcggcaagg tctggggccg
     2821 cgagttcagc ttggccgatc cgatcaggct gtacatcgcg gcagcacgtt cgccaccgca
     2881 gtcggaaccg gcgaatagtg acgggcgaca attaaaacta ccgatcgacg acaaacaaaa
     2941 ttcccgatcc aacgacaatt aggccaagcg tattccggtt catcggagcg gaggcgtatt
     3001 ggtcacagac aggcaggttc ggaggttgtt ttcgttgata aagacagagg aaagccaaga
     3061 gattgcggcg tccaaggcag gaatggacgc caagac
//
LOCUS       DASRKK010000684         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_849916, whole genome shotgun sequence.
ACCESSION   DASRKK010000684 DASRKK010000000
VERSION     DASRKK010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849916"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFC15_16515"
     CDS             <1..568
                     /locus_tag="VFC15_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM11812.1"
                     /translation="KWDGEWHITVEVFRDLGLAFAAVLVLIYVLVVGWFRSFIVPLVI
                     MAPIPLTLVGILPAHALMGAFFTATSMIGFIAGAGIIVRNSIILVDFIGLRREQGMTL
                     EHAVVDAGAVRFRPMLLTAAAVVVGASVILFDPIFQGLAISLMAGEVASTLLSRMAVP
                     VLYYMVEKRRLPAPPTLESARDASEVRV"
     gene            565..765
                     /locus_tag="VFC15_16520"
     CDS             565..765
                     /locus_tag="VFC15_16520"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZM11813.1"
                     /translation="MNIERSVRLIAGVFVLISLALGYWVSPYWYLFTAFVGLNLFQSA
                     FTNWCPMMTFLRKFGVKSAGGA"
     gene            1041..1598
                     /locus_tag="VFC15_16525"
     CDS             1041..1598
                     /locus_tag="VFC15_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM11814.1"
                     /translation="MTHRVCPWWLGYWLTCPLRRYGQDPHKILAPYVREGMTVLEPGP
                     GMGFFTLELARLVGPSGRVVAVDVQPKMIERLKHRAAKAGLLDLVDARVAPAESMGIT
                     DLQGSADFTLAFAVVHEFPDADRFFAEVAAASKPGATILLAEPSGHVKAAAFDSELQA
                     AAAVGLRLVDRASIRHSHAALLKKS"
     gene            complement(1752..1961)
                     /locus_tag="VFC15_16530"
     CDS             complement(1752..1961)
                     /locus_tag="VFC15_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11815.1"
                     /translation="MPFEVRAVNRNPNFPQSNAMDQPCRHARVQVVARDEDAEYVECM
                     ECREVFESSEFRDMDIESKIPPQDE"
BASE COUNT          349 a          581 c          604 g          480 t
ORIGIN      
        1 gaagtgggat ggcgaatggc acatcacggt cgaggtcttc cgcgatctgg gactggcctt
       61 cgccgcggtg ctggtgctga tctacgtgct ggtcgtggga tggttccgct cgttcattgt
      121 cccgctggtc atcatggcgc ccatcccgct gacgctggta ggcatcttgc ctgcgcatgc
      181 gctgatgggc gcattcttca ccgcgacgtc gatgatcggt ttcatcgctg gcgcggggat
      241 tattgtgaga aactcgatca tcctggtgga cttcatcggg ctgcgccgcg agcagggcat
      301 gacgctggaa cacgcagtcg tcgatgctgg cgcggtgcgc ttccggccga tgctgctgac
      361 cgcggcagcg gtggtggtgg gcgcgagcgt aattctcttc gacccgattt ttcagggact
      421 cgcaatttct ctcatggccg gggaagtggc ctcgacgctg ttgtcgcgca tggccgtccc
      481 ggttctgtat tacatggttg aaaaacgcag attgccggcg ccaccaacgt tagagtcggc
      541 gcgtgacgct tcggaggtac gtgtatgaac atcgaacgct cggtgcgcct gatcgctggc
      601 gtttttgtcc tgatctcact agcactcggc tactgggtct cgccctactg gtatttgttc
      661 accgccttcg taggcttgaa cctcttccag tcggcgttta ctaattggtg cccgatgatg
      721 acattcctgc gtaagtttgg agtgaaatcg gcgggcggag cttaatcgaa agttgaccat
      781 ggctggctgg attccgatca tcgcgtttct cgttgtctac gtagtgctga cgcaatggct
      841 tctgcccaag ctcggcgttc ctacttgaag gagccggttt agcgccgggt ctcggtgcga
      901 aactaagcac gtcccaaact cgtccgtagg tcaggtccct cccgcgaaaa agccgagcga
      961 accatgatcg atgaattgtc gttacgggcg ccccgctctt gggcgcgagg aatctgctgt
     1021 tcgatggctt tgagagaaaa atgacccatc gcgtctgtcc ctggtggctg ggctactggc
     1081 tgacctgccc gctgcgaaga tacgggcagg acccgcacaa aattctcgct ccctatgtcc
     1141 gcgaagggat gacggtgctg gaacccggtc cgggcatggg atttttcacg ctggaactcg
     1201 cgcgccttgt ggggccttcc gggcgggtgg ttgcggtgga cgttcagcct aagatgatcg
     1261 agcgcctgaa acaccgcgcc gccaaggccg gccttcttga cctagtggat gcacgcgtcg
     1321 ctcctgctga gtcgatgggc attacagacc tgcaaggttc ggcggacttc accctcgcat
     1381 ttgctgtggt ccatgaattc ccagatgccg accgtttttt cgccgaggtc gccgcggctt
     1441 ccaaacccgg cgctacgatt ctcctcgccg aaccaagcgg gcacgtcaag gcggctgcgt
     1501 tcgattctga actgcaagct gcggccgcgg tcggtctcag gctggtcgat cgcgcatcca
     1561 tccggcatag ccacgctgcg ctgttgaaga aaagttaggg cgcttccagt gttgctgtct
     1621 cctgtgagca agggtagagc ggcgcttcag aaggcaggca ctacccgtgc ttgcggttac
     1681 acctatgctg gaaccgactt agacacacct ccgccctctc gcacgcacta aatcgtgttc
     1741 gagcttcgtg cctattcgtc ttggggagga attttcgact cgatgtccat gtcgcggaat
     1801 tcactcgact cgaagacctc gcggcattcc atgcactcaa cgtattccgc gtcttcatct
     1861 cgagcgacaa cctggacacg ggcatgccga cacggctggt ccatggcatt gctctgcggg
     1921 aaatttgggt tcctgttgac cgccctgacc tcaaagggca ttagctaaat ttccggtatt
     1981 ttagaccgga accatccgtt gtcaaagctt ttcc
//
LOCUS       DASRKK010000685         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1045841, whole genome shotgun sequence.
ACCESSION   DASRKK010000685 DASRKK010000000
VERSION     DASRKK010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045841"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1075)
                     /locus_tag="VFC15_16535"
     CDS             complement(197..1075)
                     /locus_tag="VFC15_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZM11816.1"
                     /translation="MRVARFIWIGAAMACVVLGIAVCASAQQSATRAAQKGAAFAPLE
                     QWKAAVLAGDAAKLRALYSTNPEARVILTLGETNAQADIAFWTGLKARSVKFEIAQST
                     TPEAGSQQVAFQVEIKSAAQNKLQTIYVIDGQLWQKQGETWCIVATRRGDVTRLQQPL
                     ATNKEIYVPGLNAREEIAHALKLATQQHKRVLVVFGANWCYDCHVLDLAFRRPDVAAL
                     LTPNFEVVHVDVGEGDKNQDIMQQYQVPMARGIPAIAVLDGNGNLLYSQKGGEFEKAR
                     ALGPDDLVAFLNKWKA"
     gene            complement(1154..1597)
                     /locus_tag="VFC15_16540"
     CDS             complement(1154..1597)
                     /locus_tag="VFC15_16540"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZM11817.1"
                     /translation="MKLRIAWIGKTRSPAIQSLTAEYLKRLSHYAACDALELASEAAL
                     LKHVEKSTGRTPPVLVLLDARGKQLASEEIANFLDYQLSHDTQDLLFAIGPADGWSDT
                     TRAKAKHVLSFGKITLPHELARVVLLEQLYRGYTILKGHPYHGGH"
     gene            complement(1601..2197)
                     /gene="rsfS"
                     /locus_tag="VFC15_16545"
     CDS             complement(1601..2197)
                     /gene="rsfS"
                     /locus_tag="VFC15_16545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZM11818.1"
                     /translation="MKKDDVRAQVAIAVKACIEKKAEDISILRLDAQASGFTDYFVIC
                     SAANPRQVQAIGDEVDMQLSKLGYEPSHVEGRNQAEWLLLDYVDFVVHIFSESARKFY
                     DLERLWKTATRLQLADLEKPAPKKATTAAKRVVVKKTATKKSPSPSQKRGKDGAPSAK
                     KTATKVKSPTPSTSSGQALSQKKGKDGAPISKRTRKGL"
     gene            complement(2271..3101)
                     /gene="nadD"
                     /locus_tag="VFC15_16550"
     CDS             complement(2271..3101)
                     /gene="nadD"
                     /locus_tag="VFC15_16550"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZM11819.1"
                     /translation="MNIALFGGTFDPIHRGHLNVARAAAERFKLKEVWFVPADIPPHK
                     QKAPVTSFFHRYSMVALALAGEPDFLPSLLEAPDPEAHVERRPSYSIDTIRKVKAGLR
                     KSDRLYFLIGIDAFREIGTWYKAEELLRECDIIVAARPGYSLDDVVGSLPKGMRPKDD
                     DAVMWQKSPSLRQAQGRLYRQKRAVERGGTRRNQAGLLELPGVRLHLLPETHEDVSAT
                     QIRTATRRGTALKKLVPDAVAEYIRKEGLYRGMPHTKAKQKIGRGGSGLKRPTQAKSA
                     "
     gene            complement(3297..>3784)
                     /locus_tag="VFC15_16555"
     CDS             complement(3297..>3784)
                     /locus_tag="VFC15_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11820.1"
                     /translation="IEGASQGAGLGTQFLRHIERTRLLVHLVDVSDASGRPDPAKDYE
                     LITKELANFGAGLDSKPTIVVASKIDAVNKDKLAKLKRYCKRKKLDLYAISAVTGEGV
                     EDLKWAMAKRVEAVREAKAPVVRVELSDDSNSSLREDIDKGTSAVSRVARKSRKQIPR
                     G"
BASE COUNT          712 a         1266 c          933 g          873 t
ORIGIN      
        1 taacaagaat tcaaaaccag tctcgcgatt ccatcccggt tacttttttc gctcttctga
       61 attgaatccg atccgcgttt tccgcgcaat ccgcggtggc cctgttttcc gctccagtat
      121 tgtccgtgga gatccgtgaa atccgcggct gcgtttgtag tactggaaac ttgaaactcg
      181 aaacttgaaa cttccttcac gccttccact tattcaggaa cgcgaccaag tcatccggcc
      241 ccagcgctct cgccttctca aactcgccgc ccttttggct atagagcaag ttgccgttgc
      301 catcgaggac ggcaatcgcc ggaatcccac gcgccatcgg gacctgatac tgctgcatga
      361 tgtcctggtt cttgtcgccc tcacccacgt ccacatggac gacctcgaaa ttcggcgtca
      421 gcaacgctgc cacatcggga cgccggaatg ccaggtccag cacgtggcag tcgtagcacc
      481 agttggcgcc gaagacgacc agcacgcgct tgtgttgctg cgtcgccagc ttcagcgcgt
      541 gcgcgatttc ttcgcgcgcg ttcaatccgg gaacatagat ctctttgttc gtcgcgagcg
      601 gctgctgcaa tcgcgtaacg tcacctcgcc gggtcgcaac aatgcaccag gtttcaccct
      661 gcttctgcca aagctgtccg tcgatcacgt aaatagtttg cagcttgttc tgcgccgcgg
      721 acttgatctc cacctgaaac gcaacctgct gcgagcccgc ttcaggcgtt gtggactgcg
      781 cgatctcgaa cttgacactc ctcgccttga gcccggtcca aaatgcgata tctgcttgtg
      841 cgttggtctc gcccaacgtc aggatcaccc tcgcttcggg gtttgtgcta tacagcgcgc
      901 gcaacttcgc agcgtcccct gcgagcactg ccgccttcca ctgctccagc ggtgcaaacg
      961 ccgcgccttt ctgcgctgct ctcgtcgccg actgctgcgc actcgcgcag acagcaattc
     1021 cgaggactac gcaggccatc gctgcgccga tccaaataaa acgggctacc cgcatgaccg
     1081 aacaaactcc ctgtattcct tcgcctttcc cagtcgccag tcaccgtctt ttctagccac
     1141 tgaccacttc tcgctagtgc ccgccatggt agggatggcc cttcaatatc gtgtacccgc
     1201 gatacagctg ctccaacagc accacgcgcg ccagttcgtg cggcagcgtg atcttcccga
     1261 acgacaagac gtgcttggcc ttcgctcgcg tcgtatcact ccatccatcc gccgggccga
     1321 tggcgaacag caggtcttgg gtgtcgtgtg aaagctgata gtcgaggaaa ttcgcgatct
     1381 cttccgaggc cagctgcttg ccgcgcgcat ccagcagcac cagcaccggc ggcgtgcggc
     1441 cagtgctctt ctccacatgc ttcaacagcg cggcctccga agcaagctcc agcgcatcgc
     1501 atgccgcgta gtgggataga cgcttcagat actcagctgt gagcgactgg atcgccgggc
     1561 tgcgcgtctt gccgatccac gcaatacgga gtttcactgc ctacaaaccc tttcgagttc
     1621 gcttcgaaat gggtgcccca tcctttccct tcttttggga aagagcctgc cctgagcttg
     1681 tcgaaggggt gggactcttc accttggttg cagttttctt cgcgctgggt gccccatcct
     1741 ttcctctctt ttgggaaggg ctgggactct tcttcgtcgc agtcttcttc acaaccacgc
     1801 gcttcgcagc cgtggtcgct ttcttgggcg ccggcttctc caaatcggca agctgcaaac
     1861 gggtcgcggt cttccatagc cgctccaggt cgtaaaactt gcgcgcactt tcggagaaaa
     1921 tgtgcaccac gaaatccacg tagtccagca gcaaccactc agcctggttc cggccttcca
     1981 catgcgaagg ctcgtatccc agcttgctga gctgcatatc gacctcatcg ccgatggcct
     2041 gcacctgccg cgggtttgcc gctgaacaaa tcacgaagta gtcggtgaat ccgctggcct
     2101 gcgcatccag tcgcaatatg ctgatgtctt ccgccttctt ctctatgcaa gcctttaccg
     2161 cgatggcaac ttgcgccctc acatcatctt ttttcatgcg attaactcga tctcctaaac
     2221 ttgccaatat cctgcgtgcc agtctacggc aacaactgcg ggtgccccac tcaagccgat
     2281 tttgcttgag tgggtctttt gagtcctgac ccaccccgac caatcttctg cttcgccttt
     2341 gtatgcggca tgccgcgata cagcccttcc ttccggatgt attccgcaac cgcatcgggc
     2401 actagcttct tcagagccgt acctcttctt gtcgccgtgc gaatctgcgt ggccgaaaca
     2461 tcttcatgcg tctccggcaa caaatgcaac cggacccccg gcagttccaa caaaccagcc
     2521 tgattccgcc gggtgccgcc ccgttctacc gccctctttt ggcggtagag cctgccctga
     2581 gcttgtcgaa gggacgggct cttctgccac atcaccgcgt catcatcctt cggacgcatc
     2641 cccttcggca gcgatcccac gacatcatcg agcgaatatc ccggacgcgc cgcaacgatg
     2701 atgtcgcact cgcgcagtaa ttcctcggct ttgtaccacg tcccgatttc gcggaacgcg
     2761 tcgatcccaa tcagaaagta cagccggtcg ctcttccgca atcccgcctt caccttccgt
     2821 atcgtgtcaa tcgagtaact tggccggcgc tccacgtgcg cctctggatc gggtgcttcg
     2881 agcagcgacg gcagaaaatc cggctcgccc gccagcgcca gcgcaaccat cgagtagcga
     2941 tgaaaaaacg aggtgaccgg agccttctgc ttgtgcggag gaatgtccgc cggaacgaac
     3001 cacacttctt tcagcttgaa tcgttcagcg gccgcgcggg ccacgttgag gtgtccgcga
     3061 tggatgggat cgaacgtgcc gccaaacagt gcgatattca ttcagctcgt aactacagtt
     3121 tcagtgttca ccgcaccgcg cgcaagtaag gattcgagca ctcgccaaat aggtatctgg
     3181 catcttaact ggcggcacgg acatccagcc aagcgttgga ccgaaagcga ggtggaagat
     3241 ctcgccttta ggccagcgcc taccagccct ttatgttttg tcattccgag tgggcttcag
     3301 ccgcgaggaa tctgctttct cgactttctc gccaccctac ttaccgctga ggtccctttg
     3361 tcgatatctt cgcggagact gctgtttgaa tcatccgaca attcgacccg tacaacagga
     3421 gccttcgctt cccgcaccgc ttccacccgc ttcgccatcg cccacttcaa atcttccacg
     3481 ccctcgcccg tcaccgccga aatcgcatat agatcgagct tcttccgctt gcaataccgc
     3541 ttaagcttgg ccagcttgtc tttgttcacc gcatcgatct tcgacgccac cacgatggtc
     3601 ggcttgctat ccagccccgc gccaaagttc gccagctctt tggtaatcag ctcataatct
     3661 ttcgccggat cgggtcgtcc gctcgcatcc gaaacatcga ccagatgcac cagcaggcgc
     3721 gtccgttcga tgtgccgcag aaactgcgta cccaaccccg caccctggct ggcgccttca
     3781 atca
//
LOCUS       DASRKK010000686         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1046378, whole genome shotgun sequence.
ACCESSION   DASRKK010000686 DASRKK010000000
VERSION     DASRKK010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046378"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFC15_16560"
     CDS             complement(<1..632)
                     /locus_tag="VFC15_16560"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZM11821.1"
                     /translation="MSDNNGSNGNGTSVLKPRAEWITKRREEAARSGDNNMSQMHFAR
                     KGLLTEEMVFVAEREKVAPELIRDEVAVGRMIIPANVNHPELEPMAIGIESRCKINAN
                     IGNSAITSNVDEELKKLHTSVHYGADTVMDLSTGGGIHEIREAILRHSPVPIGTVPIY
                     EAVSRVKRIEDLTIDVMLEVIEEQAEQGVDYMTSHAGVLVEFLPMITSRIT"
     gene            complement(747..1154)
                     /locus_tag="VFC15_16565"
     CDS             complement(747..1154)
                     /locus_tag="VFC15_16565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZM11822.1"
                     /translation="MSLTDLQSVLDSAPFVQQYGFQLESADAGTCTLYCPVKASFLRP
                     DAIVSGPIFMAAADVAMWIAIISALGPDASSTVTVEMKTNFVSALVGVQDFRCAATVM
                     KSGSRLVFGTADCRSLDGRILTHHTMTYIRREK"
     gene            1409..1828
                     /locus_tag="VFC15_16570"
     CDS             1409..1828
                     /locus_tag="VFC15_16570"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZM11823.1"
                     /translation="MGENKTKPTEVSVAAFVDAITDVTKRADAKALVKLMQKASGEKP
                     KMWGPSIIGFGSIHYKYDSGREGDMPLIGFSPRKAANVLYGAIGFSGAEALLAKLGKH
                     TTGKGCLYIKKLSDVDQTVLETLVVKSLAAKHARHPS"
BASE COUNT          475 a          630 c          720 g          497 t
ORIGIN      
        1 gtgatgcggc tggtgatcat gggcagaaat tcgacgagca cgccggcgtg gctggtcatg
       61 tagtcaacac cctgctcggc ctgctcttcg atgacttcga gcatgacgtc gatggtgagg
      121 tcttcaatgc gcttgacgcg cgagaccgct tcgtaaatcg gcaccgttcc aatgggcacc
      181 ggcgagtgcc gcaggatggc ttcgcggatt tcgtggatgc cgccgccggt agagaggtcc
      241 atcaccgtgt cggcaccgta atgcaccgag gtgtggagtt tcttcagctc ttcgtcaaca
      301 ttgctggtga tggccgaatt gccaatgttg gcgttgatct tgcagcggga ctcaatccca
      361 atggccatcg gctccagctc gggatggttc acgttggccg ggatgatcat acgtcccacg
      421 gcaacttcgt cgcggatgag ttcgggcgcg accttttcgc gctcggccac gaacaccatt
      481 tcctcggtga gcaggccctt gcgcgcaaag tgcatctgcg acatgttgtt gtcgccgctg
      541 cgcgcggcct cttcgcggcg cttcgtgatc cattcggcac ggggtttcaa cacactcgta
      601 ccattgccgt tgctgccatt gttgtcgctc atgggattgg gaaacctctc cggacttacc
      661 gcctattata gcgccacgaa acgtcgcgca cggggtttcg tacgggcgaa ttgctggaca
      721 gaacgggtcc tggtaagacg ccacaatcac ttctcgcgac ggatgtaggt catggtgtga
      781 tgggtgagga tgcgaccatc gagcgagcgg cagtcggcgg ttccgaacac caggcgcgag
      841 ccggatttca tgacggtggc ggcgcaacga aaatcctgca cgccaaccag ggcggagacg
      901 aagttcgttt tcatttcaac cgtcacagta gagctggcgt cggggccgag agcgcttatg
      961 atggcgatcc acatggcaac atcggcggcg gccatgaaaa tgggtccgct cacgattgcg
     1021 tcgggtcgca ggaagctggc tttaaccgga cagtagagcg tgcacgtacc agcatcagca
     1081 gattcaagct ggaatccgta ttgctgaacg aagggcgcgc tgtcgagcac agattgcaga
     1141 tcggtcaggg acatgaacct ccgaaggtaa gagatgaagg gcgatggtaa acgatgggca
     1201 ggtgttgagc atagggtgtc ataaggcctc tcagcatcga ctaccgccgt ccaaccacgt
     1261 acagcagcac gcctccaccc accggaacag cgctgaggac tacaagtttg tcgatggtca
     1321 gctggttcac gccacgcaaa atcgtcatcc cactatgctc cgttcgggag cgatgttagc
     1381 cttgggaaga gacgggagga gacacggaat gggggagaac aagaccaagc ccaccgaagt
     1441 gagtgtggcg gcgttcgtcg atgcgatcac ggacgtgacc aagcgcgcag acgccaaggc
     1501 gttggtgaag ttgatgcaaa aggcgtctgg cgagaagcca aagatgtggg ggccgtcgat
     1561 catcggcttc ggtagcatcc actacaagta cgacagcgga cgcgagggcg atatgccgct
     1621 aattggtttt tcgccgcgta aggctgcaaa tgttttgtat ggtgctatcg ggttcagcgg
     1681 tgctgaggcg ctgctggcca agctcggcaa acatacgacc ggtaaaggct gcctgtacat
     1741 caagaagctg agtgacgtgg accagacggt cctggagacg ttggtcgtga agtcgctggc
     1801 ggcgaagcac gcgcgccatc cgagctagcg agaccagtcg tgaagtcggc cgcggcgact
     1861 ctgccttatc accagcctct gtgtcctccg tcctatcccc gtgaagttcg ctgtgtcatg
     1921 aatgccatgt gaggcgaagg gattagtgtc tcttgcctgc ggtataacct tgccctcaga
     1981 atgcgtatct cttaaccggc cgggttttga ggatttaatt gatcctcgtc atcgtcgtgg
     2041 cggtgggaat gtcctataga aggggatgtc aagagacata ttcctccctt cgccggacgc
     2101 ggtagaattt ctcgcacatc cagcccccct cgcgggttgc ctcttcgtct ggaccgcttt
     2161 tcgcggcgga gcgtcagacc gagttgttcc cattgcgacg gtttcatgac acgatccgtt
     2221 ctcgacgcca ttcggctcct gaaacttgca agcagagcgg gcgggcaaag tgccaaccat
     2281 ggtcctatct gagactcgag gcctatagct ttcttgggtt at
//
LOCUS       DASRKK010000687         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_165988, whole genome shotgun sequence.
ACCESSION   DASRKK010000687 DASRKK010000000
VERSION     DASRKK010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165988"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFC15_16575"
     CDS             complement(<1..285)
                     /locus_tag="VFC15_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZM11824.1"
                     /translation="MIQTLFGSTEEKPSFLQRMKEAVTRTRETLSERIEEVVAIRKEI
                     DRDTLDDLEATLLTADLGTATTATVLAALREKVDRKQVGNVEELKRVLKDE"
     gene            489..743
                     /locus_tag="VFC15_16580"
     CDS             489..743
                     /locus_tag="VFC15_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZM11825.1"
                     /translation="MAKCEHVKQANPNVQPSGEGCQSCIDAGLRWVQLRKCLTCGHVG
                     CCDSSAGQHANKHFKEMGHPVMQAFKSGDWKWCYVDEAYV"
     gene            1113..>3684
                     /locus_tag="VFC15_16585"
     CDS             1113..>3684
                     /locus_tag="VFC15_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZM11826.1"
                     /translation="MQLRFGNETALAAEVTEPLPQALKRGPRTDGSYARLKACSTHRL
                     YTGCETAIGALVRLCLLLALLATTAFAQVPSPWVDTKPLPIDTGDAGLKEMLRRLHTT
                     ARLMHTVAHPDDEDGGMLTLESRGHGVTALQLTLNRGEGGQNKIGSNLLDELGIVRTL
                     ELLAADRYYAVEQRFTRVVDFGFSKTPEETFQMWHGHDVALGDMVRVIRTFRPDVICT
                     RFQGNSRDGHGHHQASGILTREAFRAAADPARFPEQIREGLQPWQAEKLYMDNVRGTE
                     DWTVELETSQLDPLLGETYVQFAWKGLKHQLSQGAGAWTVPESRRTSYYKLIDSVLPN
                     SPAAGAHEKDFFDGIDSSLPGLALRLGADQAKAPWLSPQLEAIARTVEEATTAAQTND
                     QAAGKPLLRGLNLTRALVNKVEQSDLPAAEKADLLASLRTKQEQFEQAANLSLSVDLG
                     VGPGTPDGRPMAEAFPTANGDVLAAVITAGKSFLLLAKLHNGSALPLDLKQLTLDVPP
                     GWKAELFMDKVPKRIAPGDGCALTFRVTPPVNAQLTKAYFHRDNPETDAIYQIDNPEY
                     VTLALPPPPVAAHAVYSINGEEGEIRSFARTPVHDTHGEAWSMPLAVVPPFSVETLPT
                     TQIIPAGSNPSAQLSVMVRSTVAHANGTVHPEVPAGWKVEPQSATVEFNKTGEHAAEF
                     KVLPDGAQESRYQVRALLNANDRDYSEGYSLVTRPDLGGFFYYQPALQRASVVEVKVP
                     QGLKIGYLMGAGDDIPIVLRQVGLDVTLLTPDDIGHGDLTRFGTIVLGIRAYDTRDDV
                     KKYHQRLLDYVRNGGTLLVQYNTSPSEFNSGHYMPYPAQLSRERVTVEEAPVTILDPQ
                     N"
BASE COUNT          748 a         1116 c         1096 g          724 t
ORIGIN      
        1 ctcgtctttc agcacgcgct ttagctcctc gacgttgcct acctgcttgc gatcgacctt
       61 ctcgcgcaag gccgccagca cggtcgcggt cgtagcggtg ccgagatcgg cggtgagcag
      121 ggtcgcttcc agatcgtcga gggtatcgcg gtcgatctcc ttgcggatcg cgactacctc
      181 ttcaatccgc tcgctaaggg tctcgcgcgt ccgcgtgacc gcctctttca tgcgttggag
      241 gaaactgggc ttttcttcgg tactgccaaa taaggtttga atcatgaagg aatccgtcgc
      301 gaccattgcc aaatacgcct cgacggccga tttgccgctc caacgatggc gatacgtcgc
      361 gaaaatccat tatagacaag cggttgtgct gccgctgtga tgcgctgtcg caatcctgcg
      421 cgcaggattg cgggaatctg acccgcgggc cgcttcatcc aacgaagata ttcttacgag
      481 gaggcctcat ggcgaagtgt gagcacgtga agcaagccaa tccaaatgtc cagccgagcg
      541 gcgagggttg ccaatcttgt atcgatgccg gactgcggtg ggtgcagctc cgcaagtgtc
      601 tgacctgcgg gcacgtcggc tgctgcgatt cctccgccgg tcagcatgcc aacaagcact
      661 ttaaggagat gggacatccc gtgatgcagg cgttcaagtc aggcgattgg aagtggtgct
      721 acgtagacga agcgtacgtc tgattctgcg acgatcgtct gactgcccgg ctttcatttc
      781 gcgccgggcc gcatgtttgt gtactctgta cgtcagcctt tgtactagag gccatctcat
      841 aaataccggt gtggtccaaa gcgaggtacc tcaggggcta aagctcaggt cgtaacgggc
      901 catttgcggc acggctgaag ccgagcccct tcaaaaccga tttatgagat agcttctagt
      961 aattctggcc atgacaaccg atggcatttt ctaaccgtac cagcccactg cttcgcgtaa
     1021 agcattttta cacttggtgt atagggtgga gcaccgcttc agcgccgggg tccccagcac
     1081 acgccgtttt tgcgcgtgct ggggtggaag cggtgcagtt aagattcgga aatgagacgg
     1141 ctttagccgc tgaggtcacg gaaccattac ctcaggcgct aaagcgcggt ccaagaactg
     1201 acggcagtta tgcacgcctg aaggcgtgct ccacccatcg cctatacacc ggatgcgaaa
     1261 ctgctatagg tgcgcttgtc aggctttgcc tgctcctcgc gctcctggcg acgacagcct
     1321 tcgcgcaagt tccatccccc tgggtcgata ccaagccgct gccgatcgac accggcgacg
     1381 ccgggctgaa agagatgttg cgccgtctgc ataccacggc gcgtctgatg cacacggtcg
     1441 cgcatcccga cgacgaagat ggcggcatgc ttacgctcga atcccgcggg cacggtgtga
     1501 ccgcattgca gctcaccctg aatcgagggg aaggcgggca gaacaaaatc ggcagcaatc
     1561 tcctggatga actcggcatc gttcgcaccc tggagttgct ggcagccgac cgttactacg
     1621 ccgtcgagca gcgtttcacc cgcgtggtcg atttcggatt ctccaagacg ccggaagaaa
     1681 cattccagat gtggcacggc cacgatgtag cgctcggcga catggtgcga gtcattcgca
     1741 ccttccgtcc tgatgtgatc tgcactcgtt tccagggcaa cagccgcgac ggacatggcc
     1801 accatcaggc ctcgggaatt ctgacccgcg aagcgtttcg cgcggctgcc gatccggcgc
     1861 gctttcctga gcagattcgc gaagggctcc aaccctggca ggcggagaag ctgtacatgg
     1921 acaatgtccg gggtacggaa gactggaccg tcgaactgga gacctcgcag ctcgacccac
     1981 tgctgggcga aacctacgtg caattcgcct ggaaggggct gaagcaccag ctttcgcaag
     2041 gcgccggcgc atggactgtt cccgagagtc gccgcaccag ctattacaag ctgatcgatt
     2101 ctgtgttgcc aaactcacca gcagcgggcg cccatgagaa ggatttcttc gatggaatcg
     2161 acagctcgct gcccgggctg gcattgcggc ttggcgccga tcaggcgaag gctccttggc
     2221 tgagcccgca gctcgaggcg attgcgagaa cggttgagga ggccaccacc gcagcgcaga
     2281 caaacgacca ggcagcaggg aagccattac tgcgcggttt gaacctgacg cgagcgctgg
     2341 tcaacaaggt tgagcaatcg gatctgccgg ctgcggagaa agccgacttg cttgcttctt
     2401 tgcgtaccaa gcaagagcag tttgagcagg ccgcgaatct gtcattgagc gtcgacctcg
     2461 gcgtgggtcc cggcacgccc gacggacgtc ccatggcgga agcctttccc accgccaatg
     2521 gtgacgtatt ggcggctgtt atcacggcag ggaagtcctt cctgctgctg gcaaagctgc
     2581 acaacggcag cgcgcttccg ctggacctga agcagcttac gctcgacgtt ccgccgggct
     2641 ggaaagctga gctgttcatg gacaaggtgc cgaagcgaat cgcgcccggc gacggttgcg
     2701 cgctcacgtt ccgggtaacg ccgcccgtga acgcacagct caccaaggct tattttcacc
     2761 gcgacaatcc cgagaccgat gctatctatc aaattgacaa cccggagtat gtgactctcg
     2821 ccctgcctcc gccgcccgtc gcagcgcacg ccgtgtacag catcaacggc gaagaaggcg
     2881 agattcgcag cttcgcgcgc acgccggtac atgacaccca tggcgaggct tggtccatgc
     2941 cgctggccgt ggtgccgccc ttctcggtgg agaccttacc caccacgcag atcattccgg
     3001 ccggttcgaa tccttccgcg cagttgagcg tgatggtgcg ctcaaccgta gctcatgcaa
     3061 acggcaccgt gcatcccgaa gtaccggccg gatggaaggt cgaaccgcag tcggcgaccg
     3121 tcgagttcaa caagaccgga gaacatgccg cggaattcaa agttctgccg gatggagcgc
     3181 aggagtcccg ataccaagtg cgcgcgctgc tgaacgccaa cgatcgcgat tacagcgaag
     3241 gctacagctt ggtcacgcgg cccgatctgg gcggtttctt ctattaccaa cccgcgttgc
     3301 aacgcgccag cgtggtggaa gtgaaggttc cgcagggtct gaagattggc tacctcatgg
     3361 gagcgggcga cgacattccc attgtcttac ggcaggttgg cctggacgtc acgttactta
     3421 ccccggacga cattgggcac ggcgatctca cgcgctttgg gacgatcgtg ctcggcatcc
     3481 gggcctacga cacccgcgat gacgtgaaga aatatcatca gcggctcctt gactatgtgc
     3541 gcaatggcgg cacgctgctg gtgcagtaca acacctcgcc aagcgaattc aatagcggac
     3601 actacatgcc ctatccggcg caactcagcc gcgagcgcgt gaccgtcgaa gaggcgccgg
     3661 tcaccatcct tgatccgcaa aacc
//
LOCUS       DASRKK010000688         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1438338, whole genome shotgun sequence.
ACCESSION   DASRKK010000688 DASRKK010000000
VERSION     DASRKK010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438338"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..>2650)
                     /locus_tag="VFC15_16590"
     CDS             complement(182..>2650)
                     /locus_tag="VFC15_16590"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11827.1"
                     /translation="PERSMRLQVRFEIAEGADDLIARYNREQQNPNAVLQAVDREQSD
                     VWIKKNADGYYLRHMLHFYFIWDPRIHHQSPEVEWKKKMRGSSFSMSASKCIERSRRE
                     HEDLVAEFNSLMSGVEASLQATGMKIERLTQNDIFLEVKRALNPLVNDKRPFLPPREA
                     LVYESARSQMASVNIEDELDEHVKIGGLLYSFISLKDLPDATFPGVLRELVVMDFPII
                     INAEVVLPDQTKAVKQYKSRLRKMTAAQKDIHGGFRLNVDAQVAEHQLIKVLQELISS
                     SLKSCQLSLTVAVRTSRPARNRFEAEEAERTLADRRQRVLHAIGRMNGARGIPETLAQ
                     KRFFFSSLPGLGEINKREKDVLTLHAADLLPVEMPWKGTPNSPLMLFETPYRQLIPFS
                     PFDSGLGDANMLIMAKSGGGKTFLAQLKLLMMARARPLVSILERGDSYEPLVDLMGGR
                     VINVDLDGRETLNPWDLPEGESAPTNEKTAFLKNLTRHMLGDSHHSDTSLLDNVLNDA
                     IARVYKRCSIRYSNPIPTFNDLREELANWRDAEKMQRTIDEAHLAAIKLRSWTEGGIY
                     AKLFDQPTTMRLDSDWLFFNVEGLSSDPRLETAMSMLIATAMSARATGKTGRPSITVL
                     DECWFLLDSPALAPEVVQLYRTARKRNSSVWGISQTVEDFVGTEFQPRVHGPGILKNA
                     STKIIGQQPGDVTPMVNHLNLNPVAVNQVKGFGAPRKGRSAEVLLVIGEKAETTQTIR
                     IVPTPPSYWICTTFPRERKYRAWFLKKHEGKPLLQCYQDLARKFPQGLADVSPLLEEI
                     SGEVNGIARITAAMEKGNGEHSRH"
BASE COUNT          491 a          796 c          745 g          618 t
ORIGIN      
        1 cagtagccct atttgcgctt gtgccggctg cggcgctccc actagcgtca ccaccatgac
       61 tccggcaagc gtcaccgcca gtagccgcct gtggttctgg actgtcacta cgaaccaggt
      121 aaacgctgcg aaaaacgtca catacaaaag agcctgcatt tccttcaacc tcccgaagca
      181 ctcaatgcct tgagtgttct ccgtttcctt tctccattgc cgccgtaatc cgcgctatcc
      241 cattcacctc gcctgagatc tcttccagaa gcggcgaaac atccgccagg ccctgcggaa
      301 actttctggc cagatcctgg tagcactgca gcagaggctt tccttcgtgc ttcttcagga
      361 accaggcgcg gtatttcctc tcccgcggga acgtcgtgca tatccagtaa gacgggggcg
      421 tggggacgat tcgtatcgtc tgagtcgtct ctgccttctc tccaatcacc agcagcacct
      481 cggcgctgcg gccttttcgc ggtgcgccga accctttcac ctggttcacg gctaccggat
      541 tcaggttgag gtgattgacc atcggggtca cgtctcccgg ctgttgcccg atgatctttg
      601 tgctggcgtt tttcaggatg ccgggaccgt gaacccgcgg ctgaaactcc gtgcccacaa
      661 agtcttcgac tgtctggctg atgccccaca cacttgagtt ccgctttcgg gccgtgcggt
      721 aaagctgcac cacctcaggg gccagcgccg gtgagtccag caggaaccag cattcgtcga
      781 gcactgtgat gctgggacgc ccggtttttc cggtggcgcg agcagacatg gcagtcgcaa
      841 tcagcatgct catcgccgtc tcgagccggg gatcggagct gagtccctcg acgttgaaga
      901 acagccagtc actgtccagg cgcatggttg tcggttggtc gaagagcttg gcgtagatgc
      961 ccccttccgt ccacgagcgc agcttgatcg ccgccaggtg cgcctcatcg atcgtgcgtt
     1021 gcatcttctc ggcatcacgc cagttggcga gttcctcgcg caggtcgttg aaggtcggga
     1081 tgggattgga atagcgaatt gagcagcgct tgtagacacg tgcaatcgca tcgttgagca
     1141 cgttgtccag cagcgacgtg tccgagtgat gactgtcgcc caacatgtgc cgcgtcaggt
     1201 tcttcagaaa tgccgtcttt tcgttggtgg gtgcggactc gccttcgggc aggtcccagg
     1261 gattcagggt ctctctgcca tccaggtcga cattgatcac acggccaccc atcagatcga
     1321 ccagcggttc gtaagaatcc ccgcgctcga gaatcgatac cagcggacgg gcacgcgcca
     1381 tcattaagag cttgagctgc gccaggaacg tcttgcctcc gccgctcttg gccatgatga
     1441 gcatgttggc gtcgcccaac ccggaatcga acggcgagaa cggtatgagc tggcggtagg
     1501 gagtttcaaa cagcatcaac ggagaattgg gtgttccttt ccatggcatc tccactggca
     1561 gcaggtcggc tgcgtgaagg gtcagaacat ccttttcccg cttgttgatc tcacccagtc
     1621 ccggaaggct ggagaagaag aatcgctttt gcgccaatgt ctcgggaata ccgcgtgcgc
     1681 cgttcatgcg cccgatggcg tgcaacaccc tctgccggcg gtcggcaaga gtgcgttccg
     1741 cttcttccgc ttcaaagcga ttgcgggccg gccgtgaagt gcggacggca acggtcaggc
     1801 tgagctggca ggatttcagc gagctcgata tcaactcctg taggaccttg atcagttgat
     1861 gctccgccac ttgggcgtcc acattgagcc ggaaccctcc gtggatgtcc ttctgtgccg
     1921 ccgtcatctt gcgcagccgg ctcttgtact gcttcaccgc ttttgtctga tcgggaagta
     1981 caacttcggc gttgatgatg atcgggaagt ccatcactac cagttccctc aacactcccg
     2041 ggaacgtggc gtcgggaagg tctttcaggc taatgaacga gtacagcagt ccgccaatct
     2101 tgacgtgttc atcgagttca tcctcaatgt tgacactcgc catctggctt cgtgcactct
     2161 catagaccag cgcttctcta ggaggaagga acggccgttt gtcgtttacc agcggattga
     2221 gcgcgcgctt gacctcaagg aaaatgtcgt tttgcgtcag ccgctcgatc ttcatgccgg
     2281 tagcttgcaa tgaggcctcg actccggaca tcagactgtt gaactcggcg accaggtcct
     2341 catgctcccg ccggctgcgc tcgatgcatt tgctggccga catgctgaag ctcgagccac
     2401 gcatcttctt tttccactcc acctccggcg attggtgatg gattcgcggg tcccagatga
     2461 agtagaagtg cagcatgtgc cgcaggtagt agccgtctgc gtttttcttg atccagacgt
     2521 cggactgttc acgatcgacg gcctgcaaaa ctgcattggg attctgttgt tcccggttgt
     2581 aacgtgcgat cagatcatcg gctccttcgg caatctcgaa tcgcacttgc aatcgcatgg
     2641 atctctccgg
//
LOCUS       DASRKK010000689         6659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1144037, whole genome shotgun sequence.
ACCESSION   DASRKK010000689 DASRKK010000000
VERSION     DASRKK010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144037"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFC15_16595"
     CDS             complement(<1..731)
                     /locus_tag="VFC15_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZM11828.1"
                     /translation="MGLIAVKRSWLLATQKLVLGVVVMAGAVALAQPPSAFMRDASQP
                     SSQVNPDDLKNVGIDQRLDQQLPLELQFKDEAGKTVKLGDYFKAGRPVVLNLVYYTCP
                     MLCGEELAGLSSALGVLKFTPGDEYEVVTVSFNPDDTPEIATEKKKIYIDRMNERLEK
                     KTDGAGWHFLTGQQAEIKQLADAVGFHYKRDARTGQYIHSAAIMIATPQGKLAQYYYG
                     VEFSPKDLRLGMIEASRNKIGNLVD"
     gene            complement(735..1283)
                     /locus_tag="VFC15_16600"
     CDS             complement(735..1283)
                     /locus_tag="VFC15_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11829.1"
                     /translation="MATNQDDFDLLRNPAVDYDRTDLSPRGILVFLVGLLVAGIFIEL
                     VIWGMFRFLSHSPFFAKGNQSPMVNAQKLPPPKAVGVDFENTTNVNPGNFPEPRLQTD
                     DVVDMRNLLTAEHKILYPTQPFLDKNGTVHIPISEAMKLIEERGLPVKPAGLTASEVV
                     QKAAPAGKAPAAQAQPPAPGAK"
     gene            complement(1273..2475)
                     /locus_tag="VFC15_16605"
     CDS             complement(1273..2475)
                     /locus_tag="VFC15_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11830.1"
                     /translation="MSQPNLQVRRTDFAVPAEAASWQRTYLIVGVVGAIACIAGFVLQ
                     PEQFLRGYLIGFMLWLGLSLGCMALLMVQHLSGGLWGISIRRILEASSKGLPMMAVLF
                     LPILFGRNQLYPWMTDSSLTEHNSWYLNTPGWILRFLLYFATWIGMELVLNKRSAQQD
                     EPLTASNFPRFQLFSGIGLLLYALSISFASVDWVMSLDPHWGSTIYGMIFMAGEGLSA
                     LAFCIIMLTVLTGYSPFREIIKPEQFRDLGKLMLAFVMLFAYFSFSQWLIIWAGNLPE
                     EISWFLNRIHGGWGIVALMIILFHFALPFALLLSKERKKDGRRLIWLAVLLMFMRLVD
                     IYWYVVPNFAHARGHFYFSVWYLVAPVGIGGLWLAFFFYNLRQRPLLPIYEPQVPSLL
                     NQGSGHGH"
     gene            complement(2472..3131)
                     /locus_tag="VFC15_16610"
     CDS             complement(2472..3131)
                     /locus_tag="VFC15_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM11831.1"
                     /translation="MRYALAYLLAATALLLLAGCRADMQNQPYQRPLQESAFYADKRS
                     ARPLVEGVVARGDQRADAYFYTGKIGKDDGNYMPFPVTAEVMARGQQRFNIYCSPCHG
                     EAGDGNGMIVQRGLKRPPSYHNERLRKAPVGYFFDVMTNGFGAMLDYSQQVSPRDRWA
                     IAAYIRALQLSQGATQADVPAGQQVASEPPAGVVIAAPYDTQGATGQGTAAPPTQGGP
                     Q"
     gene            complement(3172..3720)
                     /locus_tag="VFC15_16615"
     CDS             complement(3172..3720)
                     /locus_tag="VFC15_16615"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZM11832.1"
                     /translation="MSHVIAAPTLYGLMAEFETPTELVHAAKAAYAAGYREMDAYSPF
                     PIEEASEAIGFHKSAVPLIVLIGGILGGLSGFGLQYWINVIAYPLNIGGKPYDSWPAF
                     IVPTFEMTILFAGLSGMFGMFALNGLPQPYHPVFHVDKFSAVTRDRFFLCVEATDPKF
                     DLVGTQQFMQGLKPLSISEVPH"
     gene            complement(3717..5093)
                     /gene="nrfD"
                     /locus_tag="VFC15_16620"
     CDS             complement(3717..5093)
                     /gene="nrfD"
                     /locus_tag="VFC15_16620"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZM11833.1"
                     /translation="MAGVEEKNVALLDDPPVLAPGQTFDSVTDTITDIVLKRPIGLGW
                     LAGLAIGMTLLAILGMAVTYLFLRGVGIWGINIPVGWGFAIINFVWWIGIGHAGTLIS
                     AILLLFKQTWRNSINRFAEAMTLFAVACAGMFPLLHMGRPWLGYWMFPYPNSMGVWPQ
                     FRSPLVWDVFAVSTYATISAVFWFVGLVPDLATMREQATNPFARKLYGALSLGWRGSA
                     RHWTRYEALYILLAGISTPLVLSVHTVVSFDFAVAIMPGWHTTIFPPYFVAGAIYSGF
                     AMVLVLAIPIRKYYGLEGMITERHLQNSAKIMLATGLIVAYGYFFETFVALYSGSTYE
                     RFMIHNRWFGPYAPLYWSLILFNILIPQVLWWPKIRSSTAWLFGISLVVLVGMWLERF
                     VIIVTSLSRDYLPSSWGHYVATRWDWATYAGTIGLFTAAIFIFIRIMPAISIFEIRML
                     LPQAEVKE"
     gene            complement(5094..>6659)
                     /locus_tag="VFC15_16625"
     CDS             complement(5094..>6659)
                     /locus_tag="VFC15_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZM11834.1"
                     /translation="TIVQPLIAPLYNGKSEYEFMEGLIGPTSTSGYEIVQKYWQGKMT
                     GDFQAAWRKALHDGFVAGTAYPAKAVAAKGSVPAASASSTGGLEVMFRRDPTIYDGTY
                     ANNAWLQETPKPMTQICWDNAVLVSVKTAEKLGLKTEDIVEIEANGHKAKGGVWRTPG
                     HPDDAITVFLGYGRSKSGRVGTDIGFNAYALRTSDTPYFTAGKITKVGDGYGFANPQG
                     WQYIDQSGIPGAEDMSDRHIVRKATLEDFIKNPNFAHEGIEAPAPEFTLYTAVGGGPL
                     YQYTEAKWGMAIDMNSCIGCKTCVVACQAENNIPVVGKDQVRRGRHMNWLRVDTYYEG
                     GAENPTAYYQPIPCQQCENAPCEVVCPVGATVHSTEGLNDMVYNRCVGTRYCSNNCPY
                     KVRRFNFLLWQDWDTEQYKMMRNPEVSIRSRGVMEKCTYCVQRITHGRITAEKENRKV
                     KDGEIVTACQQACPAGAITFGNLNDPESKVAKLRADKRNYGLLEDLNTRPRTTYMAVV
                     LNPNPELEKPERS"
BASE COUNT         1386 a         2197 c         1770 g         1306 t
ORIGIN      
        1 tggtccacca ggttgccaat cttgttgcgt gacgcctcga tcatgccgag gcgcaaatcc
       61 ttgggggaaa actccacccc gtagtagtac tgggccagct tgccttgcgg cgtcgcaatc
      121 atgatcgccg ccgaatgtat gtactgcccg gtgcgggcat cacgcttgta atgaaacccc
      181 accgcgtcgg ccagttgctt gatctccgcc tgctgcccgg tcaggaaatg ccatcccgca
      241 ccgtccgtct ttttctccag ccgctcgttc atgcgatcga tataaatctt cttcttctcc
      301 gtcgcaatct ccggcgtgtc atccggatta aagctgacgg tcaccacttc gtactcgtca
      361 ccgggagtga atttcaatac gcccaacgcg ctcgacaatc ccgccagttc ctccccgcac
      421 agcatggggc aggtgtaata caccaggttc aggactaccg gacggccggc cttgaaatag
      481 tcaccgagct ttaccgtctt gcccgcttca tccttgaact gcaactccag cggcaattgc
      541 tgatcgagcc gctgatcgat tcccacattc ttcaggtcgt caggattgac ctgcgaagac
      601 ggctggctgg cgtcccgcat gaacgcggac ggcggctggg ccaacgccac tgcgcccgcc
      661 atcaccacca ctcccagcac gagcttctgt gttgccaaca accaactgcg tttcactgcg
      721 attaatccca cttcctactt cgctcccggc gctggcggtt gcgcctgcgc tgctggagcc
      781 ttacctgccg gggccgcctt ctgcaccact tcgctcgccg tcagtcctgc cggcttcacc
      841 ggcaatccgc gctcttcaat taacttcatt gcctcgctaa tcggaatatg cacagtgccg
      901 ttcttatcca gaaacggctg cgtgggatag agaatcttgt gttccgccgt cagcaagttc
      961 ctcatgtcca cgacgtcgtc cgtctgcaac cgcggctccg gaaaattccc cgggttcaca
     1021 ttcgttgtgt tttcaaaatc aacgcccacc gccttcggag gaggcaattt ctgcgcgttt
     1081 accatcggac tctggttgcc cttggcgaaa aacggactgt gcgacaggaa gcggaacatc
     1141 ccccagatca ctagctcaat aaaaatgccg gccaccagca gtcccacgag aaacaccaga
     1201 attccccgcg gactgagatc ggtgcggtcg tagtcaactg ccggattccg taacagatcg
     1261 aaatcgtctt gattagtggc catgtcccga cccctggttc aacaaactcg gtacttgcgg
     1321 ctcgtaaatc ggcagcaaag gccgctgccg caggttgtaa aagaagaacg ccagccacag
     1381 cccgccgatt ccaaccggcg caaccaggta ccagacgctg aaatagaaat gcccgcgcgc
     1441 gtgcgcgaaa ttcggcacca cgtaccagta gatgtccacc agtcgcatga acatcaacag
     1501 cacagccagc caaatcaggc ggcggccatc cttcttgcgt tccttcgaca gcaacagcgc
     1561 gaacggtaga gcaaagtgga acaggatgat catcagcgcc acgatgcccc atccgccgtg
     1621 gatgcggttc aggaaccaac tgatctcttc cggcaggttg cccgcccaga tgatcagcca
     1681 ctgcgaaaac gagaagtagg cgaacagcat cacgaacgcc agcatcagtt tgcccaggtc
     1741 gcgaaactgt tccggcttga tgatctcgcg gaacggactg tagcccgtca gcaccgtcag
     1801 cataataatg cagaacgcca gcgccgaaag tccctctccc gccatgaaaa tcatgccgta
     1861 gatggtcgaa ccccagtgcg gatcgagcga catcacccaa tccacgctgg caaacgaaat
     1921 cgaaagcgcg tacagcagca atccaatgcc actgaagagt tggaagcgcg gaaagttcga
     1981 cgcagtcaga ggctcatcct gttgcgccga gcgcttgttc agcaccaact ccatgccgat
     2041 ccaggtcgcg aaatacagca ggaagcgaag tatccatccc ggcgtattca ggtaccagga
     2101 gttgtgttcg gtgagcgacg aatccgtcat ccacgggtaa agctggttac gtccgaacag
     2161 aatcggcagg aacagcaccg ccatcatcgg cagccctttg ctcgacgcct ccagaatccg
     2221 ccggatggaa atcccccaca gtccgcccga cagatgttgc accatcagca gtgccatgca
     2281 gcccaagctc agtcccaacc aaagcatgaa gccgatcagg tagcctcgca ggaactgctc
     2341 cggttgcagc acgaatcctg ctatgcaagc gatcgctccg acgactccca caatgaggta
     2401 ggtccgttgc caactcgccg cttccgccgg caccgcgaaa tccgttcttc gcacttgcag
     2461 gttaggttgg ctcattgcgg gcctccctgc gtcggcggag ctgccgtgcc ttgtccggtc
     2521 gcgccctgcg tgtcatacgg agccgcgatc accacgcccg caggcggctc actcgcgact
     2581 tgctgcccag ccggaacatc cgcctgggtc gcaccctggc tcaactgcag cgcccgaatg
     2641 taagcagcga ttgcccagcg gtcacgcgga gaaacctgtt gcgagtagtc cagcatcgcc
     2701 ccgaatccgt tggtcatcac gtcgaagaaa tagccgacag gcgctttccg cagccgctcg
     2761 ttgtgatacg aaggaggccg cttcaatccg cgctgcacga tcatgccatt gccgtcaccc
     2821 gcttcgccat ggcacggcga gcagtaaatg ttgaaccgct gctgtcctcg cgccatcact
     2881 tcggcggtga ctggaaacgg catgtaattc ccatcatcct tcccgatctt gccggtatag
     2941 aagtacgcat cggcccgctg atctccgcgg gccacgacgc cttccaccag gggacgcgcc
     3001 gaacgcttgt ccgcgtagaa tgcgctttcc tgcagcggac gctggtacgg ctggttctgc
     3061 atgtcagcac gacatcccgc cagcagaagc aacgccgtcg ccgccagcag atacgctagc
     3121 gcgtagcgca ttcttgccgc agaccgctga aggtcgaatt gagctagccg attaatgcgg
     3181 cacctccgaa atcgaaagcg gcttcagacc ctgcataaat tgttgcgttc caaccaggtc
     3241 gaacttggga tcggtcgcct cgacacataa aaagaaacga tcgcgcgtca ccgccgaaaa
     3301 cttgtccacg tgaaacaccg ggtgatacgg ctgtggcagt ccattcaacg cgaacatgcc
     3361 gaacatccct gacaggcccg cgaacaagat cgtcatttca aaggtcggca cgatgaatgc
     3421 cggccacgag tcatacggct tgccgcctat gttcaatgga taggcgatca cgttgatcca
     3481 gtactgcaac ccgaagcccg acagtccgcc caggattccg ccgatcagta ctatcagcgg
     3541 caccgccgat ttgtgaaatc caatggcctc gctagcttcc tcgatcggaa acgggctgta
     3601 cgcatccatc tcccgatagc ccgcggcata cgcagccttc gccgcatgca ccagttccgt
     3661 cggagtctca aactcggcca tcaagccata gagtgtaggc gccgcgatca cgtggctcat
     3721 tccttcacct ccgcctgcgg cagtagcatg cggatttcaa aaatcgaaat cgccggcatg
     3781 atgcgaatga agataaagat cgccgccgtg aacagaccta tcgttccggc gtaagtcgcc
     3841 caatcccagc gcgtggccac gtaatgtccc cacgacgatg gcaggtagtc gcggctcagg
     3901 ctggtcacga tgatcacgaa ccgctccagc cacatgccta ccagcaccac cagcgaaatg
     3961 ccgaacagcc acgccgtgct ggatcggatc ttcggccacc agagtacctg cgggatcagg
     4021 atgttgaaca aaataagcga ccagtacaac ggggcatacg gaccaaacca gcggttatga
     4081 atcatgaacc gctcgtaggt tgatccgctg tacagcgcga caaacgtctc gaagaagtag
     4141 ccatacgcca cgatcagtcc cgtcgccagc atgatcttgg ccgagttctg caggtggcgc
     4201 tcggtgatca tgccttccag tccgtagtac ttgcgaattg gaatcgccag caccagcacc
     4261 atggcaaatc ccgagtagat cgcgcccgcc acgaagtaag gcgggaaaat tgtggtgtgc
     4321 cagcctggca tgatggccac cgcgaaatcg aagctgacca cggtgtggac cgagagcacc
     4381 agcggcgtcg aaatgcccgc cagcaggatg tacagcgctt cgtagcgcgt ccagtgccgc
     4441 gccgaaccgc gccacccgag cgacagcgct ccatacaact tgcgcgcgaa cggattcgtt
     4501 gcctgctcgc gcatagtggc caaatccggc accaggccca cgaaccagaa caccgccgaa
     4561 atcgtcgcgt acgtcgatac cgcgaacacg tcccacacca gcgggctgcg gaactgcggc
     4621 cacacgccca tgctgttggg atacgggaac atccaatacc ccagccacgg acgccccatg
     4681 tgcagcagcg ggaacattcc cgcgcaagcc acggcgaaca gcgtcatggc ctcggcgaat
     4741 cggttaatcg agttgcgcca tgtctgcttg aacagcagca ggatggccga gatcaatgtg
     4801 cccgcgtggc cgataccgat ccaccacaca aagttgatga tggcgaagcc ccagccgacc
     4861 ggaatattga tgccccagat gccgacgccg cgcaggaaca ggtaggtgac ggccatgccc
     4921 agtatcgcca gcaaggtcat cccgatcgcc aggcccgcca gccatcccag tcctatcggc
     4981 cgcttcaaaa cgatgtcggt gatcgtgtcg gtgaccgagt caaacgtctg acccggcgct
     5041 aataccggcg gatcatcgag caacgctacg ttcttttcct ctactcctgc catctagctc
     5101 cgctccggct tttcaagttc aggattcggg tttaacacca ccgccatgta cgtggtgcga
     5161 ggacgcgtat tcagatcttc gagcaggcca taattacgtt tgtcggcccg cagtttcgcc
     5221 accttgcttt ccggatcgtt caaattccca aacgtgatcg cgcccgccgg acacgcctgc
     5281 tggcaggcgg tgacgatctc tccatccttt accttgcggt tttccttctc cgccgtgatg
     5341 cgtccgtgcg tgatgcgctg cacgcaatac gtgcatttct ccatcacgcc gcggctgcga
     5401 atgctgactt ccggattgcg catcatcttg tactgctcgg tgtcccagtc ctgccacagc
     5461 aggaagttaa accgccgcac cttatacggg cagttgttcg agcagtagcg cgtcccgacg
     5521 cagcggttat agaccatgtc gttcagcccc tcggtcgagt gcaccgtcgc gcccaccggg
     5581 caaaccactt cgcacggggc gttctcgcac tgctggcacg ggatcggctg gtagtacgcc
     5641 gtcggattct cggctccgcc ctcgtagtag gtatcgacgc gcagccagtt catgtgccgc
     5701 ccgcgacgca cctggtcctt gcccaccact gggatgttgt tctccgcctg gcacgccacc
     5761 acgcacgtct tgcagccaat gcaggagttc atgtcaatcg ccatgcccca cttggcttcg
     5821 gtgtactggt agagcggccc gccacccacg gcggtgtaca gcgtgaactc cggcgctggc
     5881 gcttcgatcc cttcgtgcgc gaagttgggg ttcttgatga aatcttccag cgtcgccttg
     5941 cgaacgatgt gccggtcgct catgtcttcc gcacccggaa ttcccgactg gtcgatgtac
     6001 tgccagccct gcggattcgc gaatccgtat ccgtccccaa ctttggtaat cttgcccgcc
     6061 gtgaagtaag gcgtatcgct ggtgcggagc gcgtaagcgt tgaagcctat atccgtaccc
     6121 acccgtccgc tcttgctgcg gccgtagccc aggaacaccg tgatcgcgtc atccggatgt
     6181 ccgggtgtgc gccacacgcc gcccttcgcc ttgtggccgt tggcctcgat ctcgacgatg
     6241 tcctcggtct tcagccccaa cttctcggcg gtcttcacgc tcaccagtac cgcattgtcc
     6301 cagcagatct gcgtcatcgg cttgggagtt tcctgcagcc acgcgttgtt ggcgtacgtc
     6361 ccgtcgtaaa tcgtgggatc gcgccggaac atcacttcca gcccgccggt gctgctcgcg
     6421 ctcgcggccg gcacgcttcc cttcgccgcc acagccttgg ccggatacgc agtgcctgcg
     6481 acaaatccgt cgtgcagcgc cttgcgccac gccgcctgaa agtcgcccgt catcttgcct
     6541 tgccagtact tctgcacgat ctcgtagccg ctggtggacg tcggccctat cagcccttcc
     6601 atgaactcgt actcgctctt gccgttgtac agcggagcga tcaacggctg cacgatcgt
//
LOCUS       DASRKK010000690         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_948773, whole genome shotgun sequence.
ACCESSION   DASRKK010000690 DASRKK010000000
VERSION     DASRKK010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948773"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2009)
                     /locus_tag="VFC15_16630"
     CDS             complement(<1..>2009)
                     /locus_tag="VFC15_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11835.1"
                     /translation="QSMTVISGAPPAEFGDKTSLVIDVTTRSGLGSTTPHGDITASYG
                     TFGGSNVDFNFSYGGKNWGNFIAANGLNTGRFLDPPEFVVFHAHGNEENVFDRFDYVF
                     SPADALHFNFGFTRSWFQNPNSYDNLNLGVLGPDGGSVGPTDQRSKISTYNIAPTWTH
                     TLSPNAVLNVTGYVRRDQYNYYPSANPFADLGPIQSETVAQNRTLTNAGAIANVAYTK
                     GIHNIKAGVMYEQTFLNENDAFGIVNPTLNSPCVDANGNPVSGFTDPSQCAAAGLQAN
                     TGYTPLLSCYDLTRPVPSPSSGCQSPHSTLYSFLGHTDVKELAMYVQDTITKRNWSFN
                     LGIRGDFYNGLTTATQAEPRLGISYNIQKTNTILRVSYARTLETPFNENLVLSSTGCN
                     SPVIAAIVPCVPAPLTPGFRNEFHAGLQQAFGRFLVVDGEYIWKYTHTGYDFSIFGNT
                     PIFFPIGWHNSKIPGWALRASVPVYHGFSALVVMSSVAARFFPPQTSGLGVTVGQSGT
                     PFRIDHDEAFNQTTHLQYQTPWKRGPWIGFNWRYDSGLVAGAVPCYGVLPFNNCPTST
                     MLNGQPAVDLSGLTADQEFQAGIFCGSVRATPFSPVPTPCLASQYGSNLVKIPAPNTQ
                     NDDHNPARIASRNLFDLAIGDDNLFKGDRYKWSLQVTAINLTNKYA"
BASE COUNT          417 a          542 c          630 g          420 t
ORIGIN      
        1 gcatatttgt tggtcaagtt gatcgccgtc acctgcagac tccacttgta cctgtcgccc
       61 ttgaagaggt tgtcgtcgcc gatagccagg tcaaagaggt ttcgtgatgc gatgcgcgcg
      121 ggattgtggt catcgttctg cgtgttcggc gccgggatct tcacaaggtt ggagccgtac
      181 tgcgatgcca ggcaaggcgt cggcaccgga ctaaagggag tcgctcgcac gcttccgcag
      241 aagatgccgg cttggaactc ctgatcggca gtcaatccgc tcagatcgac ggcgggttgg
      301 ccgttgagca tggtcgacgt cgggcagttg ttgaacggca acacgccgta acaaggtacc
      361 gcgcctgcca ccagtccgct gtcatagcgc caattgaaac cgatccacgg cccgcgcttc
      421 cagggtgtct ggtattgcaa gtgcgtagtc tggttgaagg cttcgtcgtg gtcaatgcgg
      481 aagggagttc cgctctggcc cacggtcaca cctagtccgc tggtttgggg cgggaagaaa
      541 cgtgcggcca cgcttgacat cactaccagc gcgctgaagc catggtacac cggcacgctg
      601 gcgcgcagcg cccatccagg gatcttcgag ttgtgccacc cgatggggaa gaagatcgga
      661 gtgttgccga agatgctgaa atcgtacccg gtatgagtgt atttccagat atattcgccg
      721 tcgaccacca ggaatcgccc aaatgcctgt tgcagaccag cgtgaaattc attgcgaaat
      781 ccgggagtca gcggtgccgg cacgcacggc acaatcgccg cgatgaccgg tgagttgcac
      841 cccgtgctgg acagcaccag attctcatta aacggagtct ccagtgtgcg cgcataagaa
      901 actcgaagaa tcgtgttcgt cttctggata ttgtaggaaa tgcccaagcg aggctccgcc
      961 tgcgtggcgg tggtgagccc attgtagaag tcgccacgaa tacccagatt gaacgaccag
     1021 ttgcgtttcg tgatggtgtc ctgaacatac atcgcgagct ctttcacgtc ggtatggcca
     1081 aggaatgaat acaaggtgct gtgcggactt tggcagccgg aggacggtga aggaaccggc
     1141 cgagtcaggt cgtagcagct caacagcggc gtgtaaccgg tgttggcctg aagtccagca
     1201 gcagcgcact gtgacggatc cgtgaagccg gagacggggt tgccgttcgc gtccacgcaa
     1261 ggtgaattga gcgttggatt cacgatcccg aaagcgtcgt tttcgttgag gaaggtctgc
     1321 tcatacatga cccccgcctt aatattgtgg atgcccttgg tataggcgac attagcgatt
     1381 gcgccggcat tggtaagcgt ccgattctgc gccacggttt ccgattggat tggtcccaag
     1441 tccgcgaacg ggttggcact cggataatag ttgtactggt cgcgccgcac atagcccgta
     1501 acattgagga cagcgttcgg gcttaatgtg tgcgtccaag tgggcgcgat gttgtacgtc
     1561 gagatcttgg accgctgatc ggtcgggccg acggaacccc cgtccggacc cagcacgcca
     1621 aggttcaagt tgtcatacga gttcgggttc tggaaccagg agcgcgtgaa gccaaagttg
     1681 aagtgcagcg cgtccgccgg cgaaaacaca tagtcgaaac ggtcgaacac gttctcttcg
     1741 ttcccgtgcg cgtggaaaac gacaaactcc ggcggatcga ggaagcggcc ggtgttgagt
     1801 ccgttggcgg cgatgaagtt cccccagttc ttgccgccgt aggagaagtt gaaatccacg
     1861 tttgagccac caaaagtgcc gtagctggcg gtgatatcgc cgtgcggcgt cgtcgaaccc
     1921 aggcccgaac gcgtggtcac gtcaatgacc agactcgttt tgtcgccgaa ctcagcgggg
     1981 ggagcgccgg agatgaccgt catcgactg
//
LOCUS       DASRKK010000691        21890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_166719, whole genome shotgun sequence.
ACCESSION   DASRKK010000691 DASRKK010000000
VERSION     DASRKK010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21890
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166719"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1506)
                     /locus_tag="VFC15_16635"
     CDS             complement(139..1506)
                     /locus_tag="VFC15_16635"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM11836.1"
                     /translation="MKTILKIIPALVLMAICSSLMVPAQDFEAIGKEVVNELVARQFD
                     KVELQFDEQMKAALPISKLPEVWDSILAQAGPFKSIVSSRATERQGLHAAIVTCEFER
                     AMLDAKVFMDAQGRVKGLFFEPASSSPGGPSGSEEWKTPSYAKPDTFHERDTKIISGR
                     WEMPGVLTLPNSKTGVPAVVMVQGSGPQDGDESIGPNKPFKDLAYGLASQNIAVLRYV
                     KRTKKYGADSKSDAPFTVKDEVTDDAIAAVALLAKVPEVNKKQIYVLGHSLGGLLAPR
                     IASEDSQVAGIIILAGTARSLGKVVVEQVKYIAGLTGTQTPEDKKQIEKAEAIEAQVD
                     GPSLKPDTVVDFFGSPIPGLYFLDLRNYNPGKTAAALKIPILVLQGGRDYQVTSADYE
                     VWKSALANDPRATFKFYPVYTHLFMPGAGTGPASPEDYSVAGNVAEDVVNDIAHWIKT
                     NAATH"
     gene            1703..2878
                     /locus_tag="VFC15_16640"
     CDS             1703..2878
                     /locus_tag="VFC15_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZM11837.1"
                     /translation="MSDITAMDTPPAPLVALTEDETLFRDTIRQFADETVRPHVKEMD
                     EKGVFEPSLIRNFFELGLMGIEVPEQYGGGGGTFFEAILAVEELSRVDASVGVIVDVQ
                     NTLVNNALMRWTTEDQKKRYLPRMCSDTVGAYALSEANSGSDAFALQTRAELKGDEYI
                     LNGQKLWITNAKEAGVFILLATVDPSAGYKGITAFIVEKEFPGFTVGKKEDKLGIRAS
                     STCELVLVDCRVPRANVLGEVGKGYKIAIETLNEGRIGIGAQMLGLARGAWEFAVKYA
                     QERKQFGRAIAEFQGIQFQIAQMATEIEAARMLLYNCARMKDAGMNFVKEAAMTKLFS
                     SQVAERVASLCVEIYGGYGFTKEYPVEKYFRDSKIGKIYEGTSNMQLQTIAKLVMSK"
     gene            2891..3265
                     /locus_tag="VFC15_16645"
     CDS             2891..3265
                     /locus_tag="VFC15_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM11838.1"
                     /translation="MLASEKIIGFVPITDAARAKSFYAEKLGLKFVSEDPFAIVFDAN
                     GNMIRLTRMKEVKPQAFAILGWQVPDIVTTVQRLQAAGVKFERYGDFMQQDELGIWSA
                     PGGSRVAWFKDPDGNTLSVSQH"
     gene            complement(3266..3904)
                     /locus_tag="VFC15_16650"
     CDS             complement(3266..3904)
                     /locus_tag="VFC15_16650"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HZM11839.1"
                     /translation="MPPSRSEVIAVRLSQQVGANGFPVEGEWARPTPISFSHDWQGKN
                     ADLQRRTEVRALWSELELYLRFRCRYCSIDVHSDAEPNGRRDHLWERDVAEVFLQPDR
                     FGEKYYKEFEVSPNGQWLDLEITPQGLRHIASGIRSSVILDEASREWVADLAIPMVAI
                     HPRFDPAQPWRVNFFRCEGVDPLRFYGAWQPTATPVPNFHVPEKFGTLRFVE"
     gene            complement(3908..5140)
                     /locus_tag="VFC15_16655"
     CDS             complement(3908..5140)
                     /locus_tag="VFC15_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM11840.1"
                     /translation="MYRQKQIEDNGKSALVGRYRNAGVFAVIVMMLWLAVAAQAQSTG
                     TQSSSPNQATQKPDQTPDEAGGPGGEVGPMAVPKKKESTDDTPPPPRPKKQADIPEYS
                     LHVDVPVVTVDARVVMKDGRPIALPPNVAKDHFKVWEDGVPQKIQTVTQSEAPITAVL
                     VVEFASTNYNFMYDALNASYMFASNLKPIDWVAVVQFDMKSHILVDFTQDKQAIFGAL
                     NTLRIPTFSETNLWDAMYDTLDRLDRLPGHKELVIVASGRDTMSHMTFDKILKKVKST
                     PNVTIYTITTGAAMRVMAENRYGSNPDFSAANMDYIMADNQMKTFAQLTGGHWYAPRF
                     MGELPEDFKEIAQSIRNQYTITYQSSNSKQDGTYRKLKVELVGPDDKPLIVKDEKNHD
                     VKYQIIARDGYTAKHEVD"
     gene            5370..7160
                     /locus_tag="VFC15_16660"
     CDS             5370..7160
                     /locus_tag="VFC15_16660"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HZM11841.1"
                     /translation="MRSVVSSALLCLLSVAAMAAAPTNKVQIMPEDQVKVGMHGVAYT
                     VFEGVKPEAMDVEILGVLRNMAGPKADVILARLHGAKVEYTGVVAGMSGSPVYVDGKL
                     VGAIAYRIGEFSKEPIAGITPAASMMEINELDKSPSPEMEAARSGTDLAASRTAGPGI
                     SVQAGQTDASYANLLKPIETPLVFSGFSEGTIRMFSQQFAAAGVVPVMGAGSVSDDKQ
                     PEPLEPGSAVSAILVRGDMSIAGTCTVTYLDATNLLACGHPLLQSGNVDMPMTKATVL
                     ATLASPANSFKIVNTTESVGSFVQDRHTGIMGRFDREPHMIPVTLNFHGTAHAKQFHY
                     EVLNNAKITPTAMMATVFNALQGMNEYGEDTTYRLTGNISVQGYPKLDVQNMYAPVDG
                     STPTAYGIAVSIGDRFGRIYENPYETPTIEGVELNFELVPERRSARLENARTDVTEAR
                     PGDTINIETLLRPYRGDSMVRQIPVRIPTATPKGNLRILVSDGETLDKLRRVPPTMSR
                     RLPLGSTIALLNKEHTNSQVYVSLLEPNPQAMVNDKVMPTLPLSVMNVMDGMRSTQDM
                     VVVGESATSEASAPVDYVVSGAQIITLNIK"
     gene            7256..7495
                     /locus_tag="VFC15_16665"
     CDS             7256..7495
                     /locus_tag="VFC15_16665"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HZM11842.1"
                     /translation="MNITLSLDDKLVKDVRKIAVERDTTLTGLVRDYLEKLALENACS
                     GRKRREREALERSFAQVRIKVGKRNWKREDLHERA"
     gene            7482..7895
                     /locus_tag="VFC15_16670"
     CDS             7482..7895
                     /locus_tag="VFC15_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM11843.1"
                     /translation="MSALEFLDTNVLVYSYDIAHRSKQEIAQNLVRRALAGDIVTSTQ
                     VLGEFAATLLHKVVPRVGPAELIVIIDALGPIPIVATDADMVRRAVEVQGRFGLHFYD
                     SLIVAAAERAGCERIWSEDLNSEQKYFGISVQNPF"
     gene            7994..10243
                     /locus_tag="VFC15_16675"
     CDS             7994..10243
                     /locus_tag="VFC15_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZM11844.1"
                     /translation="MLSSVAAAEGTRTWQQTKYDEFEKGTARGVAINSDGSLSLAPSF
                     GVLFTSPSAYLWDMVSDAEGTIYAAAGSPARVYKIAPDGKSSVIFAPQELQVQALAID
                     NNGAIYAATSPDGKVYKIIRGGPASGKAPGGAAPLESSKSGPPGESARPAVAVDASYS
                     ASVFFDPKTKYIWTLALDKQGRLYIGTGDQGEIFRVEPNGTGVVFFKSDEAQIRVLEF
                     DKSGNLLAGTDGSGLIYRISPQGEGFVLYSAPKKEITALAIDTQGDIYAAGAGDKRAG
                     SPPPQPGAPGGPTASPPKPTIIMQQGSVQTPGSAAGITAIPFPNVINVGGSDVYRISP
                     DGSPKTIWSSNEDLVYALAFDQSERLLAGTGNRGKIYAIHPGDYTDLSKASANQVTAF
                     ARAPKGGLYAATSNLGKVFLLGPGPIAEGTYESDVFDARIFSKWGRVEVRGTGNFELF
                     ARSGNVDNPDRNWSSWKKIDLAKESPVDAPPARFIQWKAVLHSGNPAPVIDHVILNYL
                     PKNVAPEVDDVTVLVGARVPPSAHTSNTTSESAAYESPLPTVPDKHSIAVKWKARDAN
                     DDQLLYSLYYRGDGESHWKLLRDDVEDRYVNLEADLFPDGGYTIRVVATDAPSHSLTD
                     ALTGEDTSSRFEVDNTPPHVEFTASRIEGGQLHLGFRATDSFSPINRAEYSIDADDWQ
                     TAEPVGQISDYKIENYDLTVPLPAPAAGKQTDAAGDEHTIVVRVFDRFDNVGVAKSVI
                     KSSPTGSSH"
     gene            10263..10853
                     /locus_tag="VFC15_16680"
     CDS             10263..10853
                     /locus_tag="VFC15_16680"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM11845.1"
                     /translation="MKLHSYLDVAGLLVCSVYCTIPLFWLVFHPFVERWRQFGRKAYL
                     AIMPAWGIFIAAAFALSWPVRHLHLYQSRVAWVIGALFLLAGLSIYRSASQGFDRTKV
                     SGLPELELGQHEQRLVITGIRTQVRHPIYLGHLCEVMGWTLATGSLALCALLVFAVIT
                     GAIMIHKEDAELEQRFGDAYRQYRDSVPALLPKFPV"
     gene            10944..12239
                     /locus_tag="VFC15_16685"
     CDS             10944..12239
                     /locus_tag="VFC15_16685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM11846.1"
                     /translation="MQFEFFVAARYLRAKRRQAVIGIITVISIVGVAAGVASLIIALA
                     INNGFRQDLQDRLLGSTSHINLLRVEADGIRNWQPLLSQLEKQPHVVGGAPAIYEQVL
                     ISRGARAQGAVLKGIIPAEENKVSDLLNSVNVGSAAALSVPQATTSSQPPSESDEPNP
                     FSARITASLAPIVLGKDMADQLGATVGSLVTVTSPQGELTPFGIVPKYARFKVIGIFT
                     SGFYDYDNTWAFIRLSDAQHLFDLSDVVSVLEFKVDNIYQAAEIGRNLEAVAGKGFMS
                     TNWMEQNRALFRALRLERVVTFITIGLIVFVAALNILISLIMMVMEKTRDIAVLVSMG
                     AKKRQVRRIFMYQGLLIGVIGTVIGLVLGYSISWAAGHYHLLTLSAEVYSIDYVPFAP
                     RFWDAVLVSVVAIGISFVATLYPSWSAARVLPAQVLRYE"
     gene            12412..13092
                     /locus_tag="VFC15_16690"
     CDS             12412..13092
                     /locus_tag="VFC15_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM11847.1"
                     /translation="MQNLKKVFRSGDSQLVLFENLSFQVRTGEMLAIVGESGAGKSTL
                     LHILGVLDSASAGDVYFAQLDLGRLSEEAAAEFRGRELGFVWQFHYLLPEFTAVENVA
                     MPLLMRGVAAQEAEQEALHWLQEVGLADRANHRSGELSGGEQQRVALARALVTRPKIL
                     MADEPTGDLDNRTADAVFELIARLHRDYRLTSIIVTHNHAFARRCDRVLHLEHGRMVE
                     VSPQSLSA"
     gene            13274..15730
                     /locus_tag="VFC15_16695"
     CDS             13274..15730
                     /locus_tag="VFC15_16695"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZM11848.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     TNRFLRSHASVESIRKQIEAHTTIREKVSTSVDLPLSNECKRVLAYAAEEAERLSHKH
                     IGTEHLLLGLLREEKCFAAEILHERGLRLSTIRDELARTSQEKAQPSQRNRESSLLSE
                     FSRDLTQSAMDNQLDPLVGRDSELERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIADGEVPSFLADKRILALDLSLIVAGTKYRGQFEERLKTIMKELMESQNSIIFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPNEVDAVKILFGIKDRYEKFHAVTYTDDAINMSVSHSSRYLPDRFLPDKAIDLIDE
                     AGARVKLRQTSLPEDITEVQKRIKFIVHRMENAIANHEFEKARFYSDEERKERDNLRA
                     LREKYHLDESSTGVVGREDIEDVVSRWTGVPIASIKEEESQKLLRIEEELHKRVISQD
                     RAISALARAIRRSRAGLKSPNRPIGSFLFLGPTGVGKTEVARTLAQFMFGSEKSLVRF
                     DMSEYMEKHSVSKLIGSPPGYVGYEEGGQLTERVKRAPYSVVLLDEVEKAHPDLFNIL
                     LQVFEDGQLTDGLGNTVDFKNAIIIMTSNIGARFLQKRTGLGFQSDAEDMVSGKVEDM
                     VRSEVKRTFNPEFLNRLDEVIIFNALTEVDLIQIVELMVNQLNQNLAQRAITVTVTED
                     ARKWILNKTVVDRSYGARPLRRALQKYIEDPLSEALIQGTFEQRPAFIEVYLESDQLF
                     YRPVGEEKTEGVLLYSAPLI"
     gene            16057..18495
                     /locus_tag="VFC15_16700"
     CDS             16057..18495
                     /locus_tag="VFC15_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM11849.1"
                     /translation="MQGFLQDLRYGLRQLRHSPGFTATAVLSLALGIGATTAVFSVVY
                     AVLLDPYPYANSERMVHLNATLKNGADRGFGLTGPQYQQFRNANSVDSAIAEDQWNLT
                     TTGGDLPEDVNAVYFTGNGFNHFGVPALLGRGLVPADAPEGQDPQPVAVLGYPFWQRH
                     FNGDHDVVGKTIQLVHKNYTIVGVAQPRFTWGDGDVYLPLKLSADPSRSYQVDTMLKP
                     GVSHATADAEFQSLFEQFAKETPKHFPADGFRLAVQGLNDQFLKDLGGTLALLLGAVT
                     LLLAIGCGNVSILLLARGTARAHEFAVRSAIGAGRARLIRQLLTESLVLSLTGAALGI
                     MLAYRTVAVIVSLLPEGSFPHEAAIRINLPVLCFSVALAILTGVFFGLSPAMQLSRPE
                     VSQVMQASTKKVTGSVRGKRLHNILVGGQIALTLLLLATAGAAIQGFMKLIHGSLGYN
                     PHNVMSVGIPVHDNTYTTWEKRSAYFDQLVQKVGSTPQVASAGISTNATPPNNGADNL
                     FEVLGGSQLQEQRARLNFVSPEYFPILQIPLLSGRIWDQSENKRGARMAVINETLAKQ
                     YFPDGNALGRSIRMPNLKGDPPMTVAIPESNDWMEIVGVVADARNDGVGKPVKPSIYI
                     PYTVWMRVSTQILVRTQGPPLSVLYDVRKQINLVDADQQVFGQTRSLEEWITSQPEYA
                     QEHLIAMLFGAFAGLALILAAVGLYSVVSYTVAQRTNEFGIRMALGAQRGDVLRMVLD
                     STAASVGSGLVLGVILSLGLNRIITSWAQGSPITIAVLLLVSALLAAVAAMACLFPAH
                     HASTIDPMKALRYE"
     gene            18519..18779
                     /locus_tag="VFC15_16705"
     CDS             18519..18779
                     /locus_tag="VFC15_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZM11850.1"
                     /translation="MSLLVYSAPWCPDCRVAKRFLEKHNIPFHEVNIESTPGAADEVI
                     RQTGKRAIPQFVHNGTWVQPYRPGEGFLYEEMSELLGVVDEG"
     gene            18901..20217
                     /gene="purB"
                     /locus_tag="VFC15_16710"
     CDS             18901..20217
                     /gene="purB"
                     /locus_tag="VFC15_16710"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZM11851.1"
                     /translation="MIARYTRPEMGHIWSDQNKFRTWLEVELAATETLADAGLVPQSA
                     AVAIREKGDFDLDRINQIEAEVKHDVIAFTTAVAEKVGPESRWLHYGLTSNDVVDTAQ
                     ALLIKQASAIIREDLKKLRDVLKRRAWEFKDTPQIGRTHGVHAEPITFGLKLANWYSE
                     MLRNIERFELAAEQMRVGKLSGAVGNGSHLDPEYEERICQRLGLNVAAVSSQVIQRDR
                     HAFYVATLAVIASTLDNIATEVRHLQRTEVREAEEFFSEKQKGSSAMPHKRNPITCEQ
                     ISGLARVVRANTQAAMENVALWHERDISHSSVERVILPDSTILVDYLLSKTTNLIATM
                     LVYPQRMMRNLESTGGLVFSGQLLLDLVEAGVLREDAYRLVQTNAMKAWEEELNFHDL
                     IMKDKEITSKVKRDTLEHAFDLKRPLRNIDKIFHRVFGETSGDWGV"
     gene            complement(20387..21136)
                     /gene="larB"
                     /locus_tag="VFC15_16715"
     CDS             complement(20387..21136)
                     /gene="larB"
                     /locus_tag="VFC15_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZM11852.1"
                     /translation="MKSEAIKILFEQVKKGELSPDEAMQQLRHLPFEDLGFANIDHHR
                     ALRVGVAEVIFGPGKTPANLAAIFGRLAEHGNNVLATRATLEQFRAVKRKFRRAEFHE
                     LARAITLVQDETVRGKGKIVVVSAGTSDIPVAEEAVVTAQVMGNEVQHVYDVGVAGIH
                     RLLAQRDALTGARVVVVCAGMEGALPSVVGGLVGVPVIAVPTSVGYGASFHGLSALLG
                     MLNSCASNVSVVNIDNGFGAGYVASIINRLP"
     gene            21247..21861
                     /locus_tag="VFC15_16720"
     CDS             21247..21861
                     /locus_tag="VFC15_16720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZM11853.1"
                     /translation="MPTAEKSAPRTLTVAVTGASGSIFVKHLLDALDRDDRVGTVNLI
                     FSDSGLRVMAEELDGAGRSDVVGKLLGRPSQKIAQQNNADIGANVASGSYRTDAMIVL
                     PCSMGTLARIANGLALQLIERAADVCLKEQRPLILCVRETPFNRIHLRNMTLAAEAGA
                     TIFPVIPAFYFRPATTDEIAREYVNRVLAHVGLPQPDAYQWKAK"
BASE COUNT         4595 a         6608 c         6110 g         4577 t
ORIGIN      
        1 cgttgagaaa ttgcagatgg gtgacccgca cattaccgcg ctgacgggga agactggggg
       61 caatacgctg gcactgctcc tcggtgatct ccatagggcg cagcataaca caaaattatc
      121 gattagtgtt aacacgccct agtgtgttgc ggcattggtc ttgatccaat gcgcgatgtc
      181 gttcaccacg tcttcggcga cattgcccgc aactgaataa tcctcagggc tagccgggcc
      241 cgttccagct ccgggcatga acagatgggt ataaaccggg taaaacttga atgtcgcgcg
      301 cggatcgttc gccagggcgc tcttccacac ctcgtaatcc gcgctggtga cctgataatc
      361 tctcccgccc tgtaggacaa gtatcggaat cttgagcgcc gccgcggtct ttccaggatt
      421 gtagttacgc aaatccagaa agtacaagcc cgggattggc gatccgaaga agtcgaccac
      481 cgtgtcgggc ttgagcgatg ggccgtccac ttgcgcttcg atggcctcag ccttctcaat
      541 ctgtttcttg tcttccggcg tttgggtgcc cgtcagccca gcaatgtact tgacctgctc
      601 caccaccacc tttcccaaag acctcgccgt acctgccaga atgatgattc cggctacctg
      661 cgaatcttcg gaagcaatgc gcggcgccag cagtcctccc aagctgtgac cgaggacgta
      721 tatctgtttc ttgttgacct caggtacctt ggccaacagg gccacggcag ctatcgcatc
      781 gtcggtaact tcgtctttca ctgtgaaggg cgcatcggac ttggagtcgg caccgtactt
      841 cttggtgcgc tttacgtacc gcagcacagc aatattctgg ctcgccaacc cgtaggccag
      901 gtccttgaac ggcttgttcg gaccaatgct ctcgtcccca tcctgtggac ccgatccctg
      961 taccataacg acggctggga caccggtctt ggaattcggc aatgtcaaga cgccgggcat
     1021 ttcccagcgc ccactgatga tcttggtgtc acgctcgtgg aaagtgtcgg gcttcgcgta
     1081 actgggagtc ttccactctt ccgaccctga cggaccacca ggagaactgg atgcgggctc
     1141 gaagaataag cccttcaccc tgccctgcgc atccatgaag accttggcgt cgagcatcgc
     1201 acgctcaaac tcgcaagtga caatcgccgc atggagtcct tgtctttccg tcgcacggct
     1261 ggaaacgata ctcttgaacg gtccggcttg cgcgagtatt gagtcccaga cttccggtaa
     1321 tttggagatt ggcaacgcgg ccttcatctg ctcgtcaaat tgcagctcga ctttatcgaa
     1381 ctggcgtgca accagctcat tcacgacctc tttgccgata gcttcgaagt cctgcgcggg
     1441 caccatcaat gaggaacaaa ttgccataag aacaagagcg ggaatgattt tgaggatcgt
     1501 cttcatagaa acgctctccg ggcgcaatcc aacgccaacc catagtaacg cggcgtcact
     1561 gcagttacac atcctgcgga ttttccggcg agggtgctcg ccagaaatct gtcaacaacg
     1621 tcgagaccgg gttatcacac gcaagcctgc gggccggtgc tagaatcgag ggttttgtac
     1681 cccatctttg aggactaatg ccatgtcaga tatcaccgca atggacacac cgcccgctcc
     1741 cttggtcgcg ctgaccgaag acgagaccct gtttcgcgat accatccggc aattcgccga
     1801 cgaaaccgtc aggccgcatg tgaaggaaat ggatgagaaa ggcgtcttcg agccttctct
     1861 gattcggaac ttctttgagt tggggctgat ggggatcgaa gttcccgagc agtacggtgg
     1921 cggtggaggt acctttttcg aagccatcct cgccgtcgag gaattgtcgc gtgtcgatgc
     1981 gtcagtgggc gtgatcgtcg acgtgcagaa cacactggta aacaacgcgc tcatgcgctg
     2041 gaccacggaa gaccagaaga agcgctatct gccacgcatg tgctctgaca ctgtgggggc
     2101 gtatgcgctg agcgaagcca actcgggttc cgacgctttc gccttgcaga cccgcgcaga
     2161 actgaagggt gacgagtaca tccttaatgg tcagaaactc tggatcacca acgccaaaga
     2221 ggcgggcgtc ttcattctgc tggcgacggt tgacccgtct gcgggttaca agggcatcac
     2281 cgcgttcatt gtggagaagg aattccccgg cttcaccgtc ggcaagaagg aagacaaact
     2341 gggcattcgc gcttccagca cctgcgagtt ggttctggtg gactgtcgcg tgcccagggc
     2401 aaacgtgctg ggagaagttg gtaaggggta caagatcgcc attgaaaccc tgaacgaagg
     2461 acgcatcggt attggcgcac agatgctagg gctggcccgt ggtgcgtggg agtttgccgt
     2521 caagtacgcc caggaacgca agcagtttgg ccgcgccatc gccgagttcc aaggtatcca
     2581 gttccagatt gcgcaaatgg cgaccgagat cgaagccgcc cgcatgctgt tgtataactg
     2641 cgcccgcatg aaggacgccg gcatgaactt cgtaaaagaa gctgccatga ccaagctctt
     2701 cagctcgcag gtggcggagc gcgtggcatc gctctgcgtt gagatctacg gtggttatgg
     2761 attcaccaag gaatatccgg tcgagaagta cttccgcgat tcgaagatcg gcaagatcta
     2821 cgaaggcaca tcgaacatgc agttgcagac gattgccaag ctggtaatga gcaagtagga
     2881 aaggagcgcc atgctcgcat cggagaagat tatcggcttc gtgccgatca ccgacgctgc
     2941 tcgcgcaaag tcgttctacg cagagaagct tgggctcaag ttcgtcagcg aggacccctt
     3001 cgccatcgta ttcgatgcca acggtaacat gattcgcctg accagaatga aggaggtcaa
     3061 gccccaggcg ttcgcgattt tgggctggca ggtgccagac atcgtaacaa cggttcagcg
     3121 actacaagcg gctggagtaa agttcgaacg ctacggcgat ttcatgcagc aggacgaact
     3181 tggcatctgg agcgcaccag ggggctcgcg cgtagcctgg ttcaaagacc cggacggcaa
     3241 cacccttagc gtttcgcagc actgatcatt cgacgaagcg caaggtccca aatttctctg
     3301 gcacatggaa atttggcacc ggtgtagcgg tcggttgcca tgctccatag aagcggagtg
     3361 ggtcgactcc ctcacagcgg aaaaaattca cgcgccacgg ttgcgcggga tcgaagcgcg
     3421 ggtgaatcgc gaccatcgga atcgctagat cggcgaccca ctcgcggcta gcttcatcca
     3481 aaatcaccga ggatcgtatc ccgctggcga tgtgcctgag tccctgcggt gtgatttcga
     3541 gatcgagcca ctgcccgttg ggactgactt caaactcctt gtagtacttc tccccaaaac
     3601 gatcgggttg cagaaaaact tccgccacat cgcgctccca aaggtggtct ctccgtccgt
     3661 tgggttcggc gtcgctgtgc acatcgatgg aacaatatcg gcagcgaaaa cgcaggtaca
     3721 actctagttc gctccataga gctctaactt ccgtccgtct ctggagatct gcgttcttcc
     3781 cctgccagtc atgggaaaaa ctgatcggag tcggccgcgc ccactcgcct tcaaccggaa
     3841 agccgttcgc gccaacctgc tggctaagac gaacggctat aacttccgat ctggaaggcg
     3901 gcaacggcta atcaacctcg tgcttggcgg tgtagccgtc gcgcgcaatg atctgatact
     3961 tcacgtcgtg gttcttctca tctttgacga tgagtggttt gtcatcgggg cctacgagtt
     4021 ccactttcag cttgcgatat gtgccgtcct gcttggaatt gctggactga taggtgatgg
     4081 tgtactgatt gcgaatcgac tgcgcgatct ccttgaagtc ctccggcagt tcacccatga
     4141 acctcggcgc ataccaatgt ccgccggtta gctgagcgaa ggtcttcatc tggttgtcgg
     4201 ccataatgta gtccatgttg gccgcggaaa aatccggatt acttccatac cggttctccg
     4261 ccatgacgcg catggcagct cccgtggtga tggtatagat ggtcacattt ggggtgctct
     4321 tgaccttctt cagaatcttg tcgaaggtca tatggctcat ggtgtctcgg ccactggcaa
     4381 cgatcaccag ctccttgtgt cctgggaggc ggtccaggcg atccagcgtg tcgtacatcg
     4441 catcccagag gttggtttcg ctgaacgttg gaatccgcaa cgtattaagt gcgccgaata
     4501 ttgcctgctt gtcctgggtg aaatccacca ggatgtggga cttcatgtcg aattggacta
     4561 cggccaccca gtcaatcggc ttcaggttgg aagcgaacat gtacgaggca ttcagcgcgt
     4621 cgtacatgaa gttgtagttg gtggaggcaa actcgaccac cagcaccgcc gtgattggcg
     4681 cctcgctttg agttacagtc tggatcttct ggggtacgcc gtcctcccag accttgaagt
     4741 ggtctttggc cacgttggga ggcaaggcta tgggccgacc gtctttcatg acgacgcgcg
     4801 cgtccaccgt aactacgggc acgtctacgt gcaacgagta ttccggaatg tcagcctgtt
     4861 tcttcggcct tggtggcgga ggagtgtcgt cggttgactc cttcttcttg gggacggcca
     4921 tcggcccaac ttccccgccg ggcccgccag cttcgtcagg tgtctggtcc ggcttctgtg
     4981 tagcctggtt tggcgacgac gactgcgtcc ctgttgactg cgcctgcgca gcaacagcca
     5041 gccacaacat catgacaatg acggcgaaca ctccggcgtt gcgatagcgc ccaacaaggg
     5101 ctgatttgcc gttgtcttct atttgtttct gacgatacat cgaccgcctc attggcataa
     5161 ttctctgcta gtataactgc accttcagcc tctgtgatcc ggcgcgactg cagtcttcag
     5221 acgtattgaa cccttagacg tctaaacagt cgcggggaat ataggtgcag gacggtcctt
     5281 ctggtcgcgt ccaggttcgt tggaacgcat gggatttcct gccggagcgt ggccttcgcg
     5341 ctttaaggct tcaggtcagg atggcaggaa tgcgtagcgt tgtttcgtcg gctctgcttt
     5401 gtcttctgtc ggtcgcagcg atggcggctg ctcccaccaa taaagttcag atcatgcccg
     5461 aagaccaggt gaaagtgggc atgcatggcg tggcctatac cgtcttcgaa ggcgtgaaac
     5521 ccgaagccat ggacgtcgaa atcctcggtg tgctccgcaa tatggcgggg cccaaggcgg
     5581 acgtcattct cgcgcgctta cacggtgcca aagtggaata taccggcgtg gtcgcgggca
     5641 tgagcggtag cccggtttac gttgacggca agctggtcgg agcgattgcc tatcgtatcg
     5701 gtgagttttc caaagagccg atcgctggaa ttactcccgc agcttccatg atggaaatca
     5761 acgagctcga caagtcgccc tcgcctgaaa tggaggcggc cagatccggt accgaccttg
     5821 ccgccagccg caccgccggg ccgggaattt ccgtccaggc aggtcaaaca gacgcgagct
     5881 acgccaacct tcttaagcca atcgaaactc ctctggtctt cagcggattt agcgaaggca
     5941 ccatccgcat gttctcgcag cagttcgccg ccgctggggt ggtccccgtt atgggcgccg
     6001 gttcggtcag cgacgacaaa cagcccgaac cactcgagcc cggttccgcc gtcagcgcca
     6061 tcctggtgcg cggcgacatg agcatcgcag gcacctgcac cgtcacctac ctcgacgcga
     6121 caaatcttct ggcctgcgga catccgcttt tgcagtccgg taacgttgac atgccgatga
     6181 ccaaggcgac ggtgctcgca acgttggcgt cccccgccaa ttccttcaag atcgtcaaca
     6241 ccaccgagtc cgttggatcg tttgtgcagg accgccatac cggcatcatg ggtcgctttg
     6301 atcgcgagcc ccacatgatc ccggtcacgc tgaacttcca cggcaccgcg catgccaagc
     6361 aattccatta cgaagtgctc aacaacgcga agatcactcc gaccgcgatg atggccacgg
     6421 tcttcaacgc actccagggg atgaacgaat acggcgaaga cacgacttac cgtttgaccg
     6481 gcaatatctc cgtccagggc tatcccaaac ttgacgtgca gaacatgtac gcgccagtcg
     6541 atggaagcac tcccacggcc tatggcatcg cggtgtcgat cggcgatcga tttggccgta
     6601 tctacgagaa tccctacgag acgccgacaa tcgaaggcgt ggaactgaat ttcgaactcg
     6661 tgcctgagcg gcgctcagct cggctggaga acgctcgtac cgacgtcacc gaagcgcggc
     6721 ccggcgatac gatcaatatc gaaactctgc ttcgcccgta ccgcggcgac agtatggtgc
     6781 ggcaaattcc tgtcaggatt ccgaccgcaa ctcccaaagg caatctgcga attctagtca
     6841 gcgacggcga aaccctcgac aaactccgcc gcgttccgcc gactatgagc cgccgtcttc
     6901 cgctgggctc aaccattgcg ttgctcaata aagagcacac caactcgcag gtttacgtgt
     6961 cgctgctgga gccgaatccc caggctatgg tgaacgataa ggtgatgccg actctgccgc
     7021 tctccgtcat gaacgttatg gacgggatgc gaagcacgca ggacatggtc gtggtcggcg
     7081 aatcggccac cagtgaagcc tcagctcccg tggattacgt ggtttccggc gcccagatca
     7141 tcacgctgaa catcaagtga cagtaagttc aagtaactta gcgtctcgat ccggatgggt
     7201 gcccatcgac tgagcatggc ttgcactgat taagggtttc ccttattctg tgaccatgaa
     7261 tattactctc tccctcgacg acaaactggt caaggacgtg cggaaaatcg cggtggagcg
     7321 agacaccaca ctgaccgggt tggttcgtga ctacctggag aaacttgctc tcgaaaacgc
     7381 ctgctcgggt cgaaagcggc gcgaacgcga ggcgctggaa aggagcttcg cgcaagttcg
     7441 aatcaaggta ggaaagcgga attggaagcg cgaagacctg catgagcgcg cttgagttcc
     7501 ttgacacaaa tgtgttggtt tacagttacg atatcgccca ccggagcaag caggaaattg
     7561 cgcagaattt ggttaggagg gcattggctg gcgacattgt gacgtctacg caggtattgg
     7621 gcgagtttgc tgcgacgctg cttcacaaag tcgtgcctcg ggtcggtcca gcagaattga
     7681 tagtcatcat tgacgcgctg ggaccgattc cgattgttgc cacggatgcc gacatggtcc
     7741 gccgagccgt ggaagtgcag ggccgttttg gcttgcactt ctacgattcc ctgatcgttg
     7801 ccgctgcgga gcgggcgggc tgcgaaagga tttggtcgga agacttaaac tccgaacaaa
     7861 aatacttcgg catatctgtg cagaatccat tttgagcaac gttttccgcg ccccagaccg
     7921 gcttcaaaat ttgcttacat ccgacccatc tacgatcatg tcgagattcc atcgatttgc
     7981 gttggtctgc cttgtgctgt cttcagtcgc agcggcggaa ggcacccgca cctggcagca
     8041 aaccaagtac gacgagtttg aaaagggcac tgcgcgtgga gtcgccatca acagcgatgg
     8101 aagcctctcg ctggcgccat cgttcggcgt tctcttcact tcgccctcag cctatttatg
     8161 ggacatggtc tccgatgccg agggcaccat ctacgccgca gccggatcgc ccgcgcgcgt
     8221 gtataagatc gcgcccgatg gcaagtcgag tgtcatcttc gcgccccagg aattgcaggt
     8281 tcaggcactg gccatcgata ataacggcgc aatttacgcc gcaacatctc ccgacggcaa
     8341 ggtgtacaag attatccgcg gaggacctgc ttccggcaaa gcgcccggag gggcagcccc
     8401 gctggaaagc tccaagtcag gcccgccagg cgaaagtgcg cggcctgcgg tcgcggtcga
     8461 tgcttcctac agcgccagtg tgtttttcga tcccaagact aagtacattt ggacacttgc
     8521 actcgacaag caggggcgac tctacatcgg caccggcgac cagggtgaga tatttcgcgt
     8581 ggaaccgaac ggaactggcg tcgtgttctt caaaagcgat gaagcccaga ttcgcgtgct
     8641 cgagttcgac aaatcaggca acctgctcgc aggaaccgat ggcagcggct tgatttatcg
     8701 catttcacca cagggcgaag gcttcgtgct ctacagcgca ccgaagaagg agatcaccgc
     8761 tctcgccatt gacacccagg gcgacatcta cgctgctggc gctggcgata aacgggcagg
     8821 aagtcccccg cctcaaccgg gtgcccccgg tggtcctacg gctagtcctc ccaagccgac
     8881 cattatcatg cagcagggtt cggtgcagac gccgggttcg gctgcaggca tcaccgccat
     8941 tccgtttccc aacgtgatta atgttggtgg ctccgacgtc tatcgcattt cgcccgatgg
     9001 atcgcccaag acaatctggt cgtcgaacga agacctggtc tacgcgctcg ccttcgacca
     9061 gtcggaacgc ttgctcgcag gcaccggcaa caggggcaag atatacgcca tccaccctgg
     9121 cgactacacc gatctttcta aagcaagcgc caatcaggtg acggcatttg cgcgcgcgcc
     9181 gaagggcggt ctctacgctg caaccagcaa tctcggcaaa gtctttctgc ttggtcccgg
     9241 cccgattgcg gaaggcacct acgaaagcga cgttttcgat gccaggatct tctccaagtg
     9301 gggacgggtc gaagttcgcg gtacgggcaa cttcgagctc ttcgctcgca gcggcaatgt
     9361 agataatccc gaccgcaact ggagctcgtg gaagaagatt gacctggcca aggagagtcc
     9421 agtggacgcg cctcccgcgc gattcatcca atggaaggct gtccttcatt ccggcaatcc
     9481 tgctccagtg atcgaccacg tgatcctgaa ttacctcccc aagaatgtcg cccctgaagt
     9541 cgatgacgtg accgtgctgg tcggggcacg cgtacccccg tcagcgcaca ccagcaacac
     9601 cacatccgag agcgcagctt acgagtcgcc ccttcccacc gttcccgaca agcactcaat
     9661 cgcggtaaag tggaaagccc gtgacgccaa cgacgatcag ttgctctaca gcctgtacta
     9721 ccgcggagac ggcgagtctc attggaagct gctgcgtgac gacgtcgaag accgctacgt
     9781 caacctcgag gctgatcttt tccctgacgg cggatatacg attcgcgtcg ttgctaccga
     9841 cgcaccatcg cactcgctca ccgacgccct gactggcgag gatacgagca gccgcttcga
     9901 ggtggacaac actccgccgc acgtcgaatt cacggccagc cgtatcgaag gcggccagtt
     9961 gcaccttggg ttccgcgcaa cggacagctt ttcgccgatc aatcgcgccg agtactccat
    10021 cgatgccgac gactggcaga ctgccgaacc ggttgggcaa atctccgatt acaagatcga
    10081 gaactacgat ctgaccgttc cacttcctgc gccggcggcc gggaagcaaa ccgacgctgc
    10141 gggcgacgag cacaccatcg tggtccgagt attcgaccgc ttcgataatg tcggagtcgc
    10201 caagtcagtg atcaagtcat ccccaaccgg ttcctcgcac tgaagcaatc aagccgcgcc
    10261 cgatgaaact gcactcctat ctcgatgtcg ccggactcct ggtctgctcg gtgtattgca
    10321 ccatcccgct tttctggctg gtgttccatc ccttcgtgga gcggtggcgg caatttggtc
    10381 gtaaggccta cctggctatc atgcccgcat ggggaatctt catcgcagca gctttcgcgt
    10441 tgagctggcc cgtccggcat ctgcacttgt accagagcag ggtcgcgtgg gtcataggcg
    10501 cgctgttcct cctggcgggc ttgtcgatct accggtcggc ttcacaaggc ttcgatcgga
    10561 cgaaggtctc cggcctcccc gaactcgaac tgggtcagca cgagcagcga cttgtcatca
    10621 ccgggattcg cacccaggtg cggcatccga tttacctcgg ccatctctgc gaagtcatgg
    10681 gatggacact cgccaccgga tcgcttgcgc tgtgcgcctt gctggtattt gcggtaatca
    10741 ccggcgccat tatgatccac aaggaggacg ccgaactgga gcagcgcttc ggcgacgcct
    10801 accgccagta ccgtgacagc gttcctgcac tgctgccgaa gttccccgtt tgacgtacag
    10861 cccgagcgac tcgtcagttt ggagctccca cttcgctaca atgaattctg gccgatgcaa
    10921 ggttgcggcc ggtgccccta tcgatgcaat ttgaattttt cgtcgccgcg cggtatctcc
    10981 gggccaagcg ccgccaggcg gtcatcggca tcatcactgt catttcgatc gtaggagtcg
    11041 cggccggggt cgcgtcgctc atcatcgcgc tggccatcaa caacggattc cgccaggacc
    11101 ttcaagaccg cctgcttggc tctacctccc acatcaattt attgcgcgtg gaagccgacg
    11161 gaatccgcaa ttggcaaccg ttgctctctc agctagaaaa gcagccgcat gttgtcggcg
    11221 gggctcccgc catttacgag caggtactga tctcgcgcgg ggcgcgcgcc cagggagcag
    11281 tgctcaaagg catcattccc gcagaagaaa acaaggtcag cgatctgctg aactccgtca
    11341 acgtaggatc agccgctgcg ttgtctgtgc ctcaagcgac gacgtcaagt caacctccat
    11401 ctgagtccga cgagcctaat cccttctcgg cccgcatcac cgcttctctg gccccgattg
    11461 ttctcggcaa agatatggcc gaccaacttg gtgccaccgt gggatcgctg gtcacagtga
    11521 ccagcccgca gggcgaactc acaccctttg gcatcgttcc caagtacgcc cgattcaagg
    11581 tcatcggcat tttcacttcg ggcttttacg actacgacaa cacctgggcg ttcattcgcc
    11641 tgtccgacgc acagcacctc ttcgatctga gcgatgtcgt ttccgtgctc gagttcaagg
    11701 tcgacaacat ttaccaagct gccgaaatcg gacgcaacct ggaagccgta gccggtaagg
    11761 gcttcatgtc gaccaactgg atggaacaga accgcgctct ctttcgcgcc ctgcgtctcg
    11821 aaagagtcgt caccttcatc accattgggc tgattgtctt cgtcgccgcc ctcaacatcc
    11881 tgatctcgct gatcatgatg gtgatggaga agacccgcga catcgctgtg ctggtctcca
    11941 tgggagccaa gaagcgccag gtccgccgca tcttcatgta tcagggattg ctgatcggcg
    12001 tgatcggaac cgtaatcggg ctggtcctcg gctactcgat ctcctgggct gctgggcact
    12061 atcatctgct cacgctctcc gccgaggtgt attccatcga ctacgtccca ttcgcgccgc
    12121 gcttctggga tgccgtgctg gtgtccgtcg tcgcgatcgg catttcgttc gtcgccaccc
    12181 tctacccatc atggtctgct gcccgagtat tgccggccca ggtgctccgc tacgaatagg
    12241 cgaccaattc tgacgtagtt caatcctgat tttgccggca atgcggcgta actgtcactg
    12301 acgcaactgc aaaagccgtg ttctaattta aggagtctgg cggctattcg gccggatgct
    12361 gagacgtaag gagcaattct gcatctaatc gtacaagctg agttgttgcg agtgcagaac
    12421 ctcaagaaag tgtttcgttc gggcgactct cagctcgtgc tctttgagaa tttatcgttc
    12481 caggtgagga ccggggagat gctcgctatc gttggcgagt caggtgccgg aaagagtacc
    12541 ttactgcata tcctcggagt ccttgatagc gcttccgcgg gtgacgtata cttcgcccaa
    12601 ctcgatttgg ggaggctctc ggaagaggct gccgcggaat ttagaggacg ggaactcgga
    12661 ttcgtctggc agtttcatta tctgctgccc gagttcaccg cggttgagaa cgtggccatg
    12721 ccgttgttaa tgcggggcgt agccgcgcag gaagcggaac aagaagccct gcactggttg
    12781 caggaagtgg ggttggcgga ccgagccaat catcggtccg gcgaattgtc tggaggagag
    12841 cagcaacggg tagcgctggc ccgtgcgctg gtcaccagac cgaaaatcct gatggccgac
    12901 gagccaacgg gagacctcga caaccgtaca gcggatgcgg tatttgaatt gatcgccagg
    12961 ttacatcgcg attaccggct tacctcgatc atcgttactc ataaccatgc gttcgcgcgt
    13021 cgttgtgacc gggtgcttca tctggagcac ggccgaatgg tcgaggtgtc accgcaatcg
    13081 ctgtcggcct gatgcaacag gacgactgaa tgcgtgtttg tagtattcga ggtcctctaa
    13141 acaaggtcct catcattcgg tctcggtaat ttttctgagg ccggcggtca ggggaaaggc
    13201 agcaacggaa agctccaggg atcggtagat cagaattacg ggaaacgtcc caaagagctt
    13261 ttggaggggt ccaatgttcg aacggtacac ggaaaaggcc cgacgcgtta ttttcttcgc
    13321 acgttacgaa gccagccagt ttggttctcc gtacattgaa acggagcact tgctgctggg
    13381 acttctgcgc gaagacaagg cgctcaccaa tcggtttcta cggtcgcacg cttcggtgga
    13441 gtccatccgc aagcagatcg aggcccacac caccatacgg gaaaaagtat cgacctccgt
    13501 tgacctcccc ctgagcaacg agtgtaagcg cgtactggcc tacgcagccg aagaagccga
    13561 gcgcctttcg cacaagcaca ttggcaccga gcaccttctg ctgggactgc tccgcgaaga
    13621 gaagtgcttt gctgcggaga tcctgcacga gcgcgggttg cgcctctcga ccattcgcga
    13681 tgaactggcg cgcacctcgc aggagaaggc acagccttcg cagcgcaatc gcgaatcttc
    13741 tctgttgtcc gagttctccc gcgacctgac gcagtcggcg atggacaacc agcttgatcc
    13801 gctcgttgga cgcgacagcg aactcgagcg cgtggtgcag atcctctgcc gccgcaccaa
    13861 gaacaatccc gtgttgatcg gcgagcccgg cgtcggcaag accgccatcg tcgaaggctt
    13921 ggcacagcgc atcgccgacg gcgaggtgcc ctcgttcctg gcggacaaac gcatcctcgc
    13981 acttgacctg tcgctgatcg tcgccggcac caagtatcgc ggccagttcg aagaacgtct
    14041 caagaccatc atgaaggagt tgatggagtc acagaattcc atcatcttca tcgacgagtt
    14101 gcacacgctg gtcggtgccg gctcggcgga aggatcgctc gacgccgcca acatcctcaa
    14161 gcctgccctc tcgcgcggcg agattcagtg catcggcgca accacgccgg ctgagttccg
    14221 caagtcgatc gagaaggacc gctccctgga gcgccgtttc caggccgtga aagttccacc
    14281 tcctaacgaa gtggacgcgg tgaagatcct gtttggcatc aaggatcgtt acgagaaatt
    14341 tcacgccgtc acctacactg acgatgcgat caacatgtcg gtgtcgcatt ccagccgcta
    14401 cctgcccgac cgcttcttgc ccgacaaggc catcgatctc attgacgagg ccggagcgcg
    14461 tgtgaagttg cggcagactt ccttgcccga agacatcacc gaggtgcaga agcgcatcaa
    14521 attcatcgtg catcgcatgg aaaacgccat cgcgaaccac gagttcgaga aggcgcggtt
    14581 ctactccgac gaagagcgca aagaacgcga caacctgcgc gctctgcgtg agaagtatca
    14641 cctcgacgag tcctccaccg gcgtcgtggg ccgcgaggac atcgaggacg tcgtctcccg
    14701 ctggaccggc gtgcctatag cttccatcaa ggaagaagag agccagaaac tcctccgcat
    14761 agaagaagag ctgcacaagc gggtcatctc gcaggaccgc gccatttcgg cgctggctcg
    14821 cgccatccgc cgttcacgtg ccggactgaa gagcccgaac cggcccatcg gttcgttcct
    14881 cttcctcggc cccacgggtg ttggcaaaac cgaagtggct cgcaccctgg cgcagttcat
    14941 gttcggcagc gagaagtctc tggtgcgctt cgacatgtcg gaatacatgg agaagcactc
    15001 ggtctccaag ctcatcggct cgcctccggg atatgttggc tacgaagagg gaggccagtt
    15061 gaccgagcgc gtcaagcgcg cgccttactc cgtcgtgctg ctcgacgaag tggagaaggc
    15121 gcatcctgac ctcttcaaca tcctgttgca ggtgtttgaa gacggccagt tgaccgacgg
    15181 actcggcaac accgtggact tcaagaacgc catcatcatc atgacctcca acattggcgc
    15241 ccgctttctg cagaagcgta ccggtctggg ctttcagtcc gacgcggaag acatggtttc
    15301 gggcaaagtc gaggacatgg tgcggagcga ggtgaagcgc accttcaacc cagagttcct
    15361 caaccgtctc gacgaagtca tcatcttcaa cgcgctgact gaggttgacc tcattcagat
    15421 cgtcgagttg atggtgaacc agctcaacca gaacctggct cagcgtgcga tcacggtcac
    15481 agtgaccgaa gatgcccgca agtggatcct caacaagacg gtcgtcgatc gcagctatgg
    15541 tgcgcgtccc ctgcgtcgcg cactgcagaa gtacatcgag gacccgctgt cggaggcgct
    15601 catccaggga accttcgagc agcgtccagc gttcatcgag gtctatctcg aaagcgatca
    15661 gctcttctac cgccccgtcg gcgaggagaa gacagagggt gtcctgctgt acagtgcgcc
    15721 gctgatctag aacgagctat cacaagaaac caagagggag cgacatcgct ccctcttttt
    15781 cttttgtcat cttgagggaa gtgggcccgc cacagcgggc gaacgcaatc gaaggagccc
    15841 tataatcgac atggaagcca cttcggcaag aagactcctc aagcctcacg gctcaatgtc
    15901 cgctcctgaa cactgtctcc gaatccgaac agccatcccc acctcgctgc ccatcgtccc
    15961 accttatctc tctttgctac caatcagatg cctaagctgt cgggtggccg tctaactgca
    16021 acgtgtcaat cggataggtt tcgctgaggt gatggaatgc aagggtttct ccaggatctt
    16081 cgttacggac tgcgccagct tcgccatagc ccgggattca cggccactgc agtcctctcg
    16141 ctggctctcg gcattggcgc cacgactgcg gtattcagcg tagtctatgc ggtcctgctc
    16201 gatccctatc cctacgctaa ctccgagcgc atggtccacc tcaatgcgac actcaagaac
    16261 ggcgcggacc gagggttcgg tctcaccggc ccgcaatatc aacagtttcg caacgcaaac
    16321 tccgtggaca gcgcgatcgc cgaagaccag tggaacctga ccacaacggg cggtgactta
    16381 cccgaggacg taaatgcggt gtacttcacg ggaaacggct tcaatcactt tggcgtgccc
    16441 gcattgcttg gccgaggatt ggttcccgcc gatgctcccg aaggtcagga cccgcaaccg
    16501 gtggctgtgc tcggctatcc gttctggcag cgtcacttca acggcgatca tgacgtagtc
    16561 ggcaagacca ttcagctggt tcataagaac tacaccatcg tcggcgtagc ccagccgcgc
    16621 ttcacctggg gagatggcga cgtttatctt ccgctgaagc tgtccgccga tcccagccgc
    16681 agctaccagg tcgacaccat gctgaagcct ggtgtaagtc acgccacagc tgacgctgag
    16741 ttccagtcgc tctttgagca attcgccaag gagaccccaa agcattttcc ggctgacggt
    16801 ttccgcctgg ccgtgcaagg gctgaatgat cagttcttaa aagacttggg cgggacactg
    16861 gccctgttgc tgggcgccgt gacgctcttg ctggccattg gttgcggtaa tgtttccatc
    16921 ctactgctcg cgcgcgggac ggcgcgtgcc cacgaattcg ctgtacgttc tgccatcggg
    16981 gccggacgtg ctcgcctgat acggcagctc ttgaccgagt ccctggtctt gtcactcacc
    17041 ggcgcggctt tggggatcat gttggcctat cgcaccgtag cggtgatcgt ctctctgctt
    17101 cccgagggct ctttccccca cgaagcggct atccgcatca accttccggt cctctgcttc
    17161 agcgttgcgc tcgccatcct caccggcgtc ttcttcggac tctcaccagc gatgcaactg
    17221 agtcgccccg aggtttcgca ggttatgcag gccagcacaa agaaggtcac cggaagcgtc
    17281 cgcggcaaac gcctgcacaa tattctcgtt ggcggccaga tcgctctcac acttttgttg
    17341 ctcgctaccg ccggtgccgc catccagggt ttcatgaagt tgattcatgg cagtctcggt
    17401 tacaatccgc acaatgtcat gtccgtcggc atcccggtgc acgacaacac gtacaccact
    17461 tgggaaaagc gctctgcgta cttcgaccaa ctggtgcaga aagtcggctc gacgccacaa
    17521 gtggcctccg cgggaatttc caccaatgcg acgccaccca acaacggggc cgacaacctc
    17581 ttcgaggttc ttggcgggtc gcaactgcaa gagcaacggg cacggcttaa tttcgtgagc
    17641 ccggaatact ttcccatcct gcaaattccg ctgctctcag gccgcatctg ggaccagtca
    17701 gagaacaagc gcggcgccag gatggccgtt atcaatgaga ctcttgccaa gcagtatttc
    17761 cccgatggca atgccctcgg ccgcagcata cgcatgccca acctgaaggg cgatcccccg
    17821 atgaccgtgg cgattccaga aagcaatgac tggatggaga tcgtcggggt ggttgcggat
    17881 gcgcgcaacg acggcgtcgg aaaacccgtg aagccctcca tttacatccc ctataccgtc
    17941 tggatgagag tgtcgaccca gattctggtg cgcactcagg ggccgccact ctcggtcctg
    18001 tatgacgtgc gcaaacagat taaccttgta gacgccgatc aacaggtctt cggacaaact
    18061 cgcagcctgg aggaatggat caccagccag ccggagtacg cccaggaaca cctgatcgcc
    18121 atgctgtttg gagctttcgc gggactggcg ttgatactgg ctgcggtggg cctgtacagc
    18181 gtcgtgtcct acactgtggc acaacgtacc aacgagtttg gaatccgcat ggccttgggc
    18241 gcccagcgcg gcgatgtgct gcgtatggtc cttgactcga ccgccgcaag cgtgggtagc
    18301 ggactggtct tgggtgtgat cctgagcctg ggcttgaatc gcatcattac ttcctgggcc
    18361 cagggtagcc ccatcacgat cgcagttctg ctcctggttt ccgcccttct ggccgcggtt
    18421 gctgcaatgg cgtgcctctt cccagcgcac catgcttcaa ccatcgatcc gatgaaggcg
    18481 ctgcgttacg agtaagactc atttatcatg aaagagacat gagcttgctc gtttattcgg
    18541 ccccctggtg ccccgactgt cgcgttgcca agcgcttcct tgagaagcac aacatcccat
    18601 tccatgaagt gaacatcgaa tccactcccg gcgccgctga cgaagtcatc cgtcaaaccg
    18661 ggaagcgcgc catcccgcaa tttgtgcaca acggtacatg ggttcagccc taccgccccg
    18721 gcgaaggctt cctatacgaa gagatgagcg aactactggg cgtggtggac gagggctaag
    18781 agagcgccac tgcttgcaga tccgcccgca tccttactcg ccaacccgct ctgagttcac
    18841 caacatttac aataaagggt tgtccatcgc gcagaatcgc gacctgtaaa ggagcccgcc
    18901 ttgatcgcac gttatacccg gcccgagatg gggcatatct ggagcgacca gaacaagttc
    18961 cgcacctggc tcgaggtcga actggctgcc acggaaacgc tggccgacgc cggtttggtt
    19021 ccgcaatcag cggctgtcgc catccgcgag aagggtgact tcgacctcga ccgcatcaac
    19081 cagatcgaag ccgaagtcaa gcacgacgtc atcgctttca ctaccgccgt cgccgaaaag
    19141 gttggccctg aatcgcgctg gctacattac gggctcacct ccaacgacgt ggttgacacc
    19201 gcgcaggccc tgctgatcaa gcaggcctca gccatcattc gcgaagacct taagaaattg
    19261 cgtgacgtgc tcaaacgccg tgcctgggag ttcaaagaca cgccgcagat cggccgaacc
    19321 cacggtgttc acgccgagcc catcaccttc ggactgaagc tggccaactg gtactccgaa
    19381 atgctgcgca atatcgagcg atttgaactg gcggccgagc agatgcgtgt cggcaagctt
    19441 tccggagccg tcggcaatgg ctcccatctc gatcctgaat atgaagagcg gatttgccag
    19501 cgtctgggac taaatgtcgc ggcagtgtct tcgcaggtga tccagcgcga ccgccatgcg
    19561 ttctatgttg cgacgctcgc cgtcatcgct tcaaccctcg acaacatcgc caccgaggtt
    19621 cgccacttgc agcgtaccga agtccgcgag gccgaagaat ttttcagcga gaagcagaag
    19681 ggctcgtcgg ccatgccgca caagcgcaat cccatcactt gcgaacagat cagcgggctg
    19741 gcacgcgtgg tgcgcgcgaa tacccaggct gcgatggaaa acgttgccct gtggcatgag
    19801 cgcgacatct cccactcgtc cgtggagcgc gtcatcctgc ccgattccac tatcctcgtc
    19861 gattatctgc tgagcaaaac aaccaacttg atcgccacca tgctggtgta tccgcaacgc
    19921 atgatgagaa accttgaatc gacgggcggg ctggtgttca gcggacagct gctgctcgac
    19981 cttgtcgaag ccggtgtcct gcgcgaagac gcctatcgcc tcgtccagac caatgccatg
    20041 aaggcgtggg aggaagaact gaacttccac gatctaatca tgaaggacaa ggagatcacg
    20101 tcaaaggtga agcgcgacac gctggagcac gccttcgacc tgaagcgccc actccgcaac
    20161 atcgacaaga tattccaccg cgtcttcggc gaaaccagcg gcgactgggg cgtctagggt
    20221 cccatctcgc aaacttccaa gccttcgcaa gaagtgacga attcaccaca gagacacgga
    20281 gagcacggaa ttagtttctc ttttgctctg tagtcttctc cgtgtcctct gtgactccgt
    20341 ggtgaaaaat tcagccacgg ttgggccttc gttgcgatgt gatcgactac ggcagccgat
    20401 taatgatcga cgcaacatat ccagcgccaa acccattgtc gatgttcacc accgatacat
    20461 tcgacgcgca agagttcagc atccccaaca gcgctgacaa gccatggaac gacgctccat
    20521 agccaacgct ggtagggact gcaatcacgg gaacaccaac caatccgccg accacactgg
    20581 ggagcgctcc ttccattccc gcacacacta ccaccacgcg cgccccagtc agcgcatcgc
    20641 gctgggccag tagccgatgg atgcccgcca cgccaacgtc atagacatgc tgcacctcgt
    20701 tacccatcac ttgcgcggtg accaccgcct cttccgccac cggaatgtcg ctggtgcccg
    20761 ccgataccac gacgatcttt cccttgcctc ggacggtctc atcctgtact agagtgatgg
    20821 cgcgcgccaa ctcatgaaac tcggccctgc gaaacttgcg cttcaccgcc cgaaattgtt
    20881 ccagcgtcgc acgagtggcg agcacattat tgccgtgctc tgcgagtcgc ccgaagatcg
    20941 cggctagatt cgcaggcgtt ttcccaggcc cgaagatcac ctccgccacg ccaacccgca
    21001 gcgcacgatg atggtctatg ttggcaaatc ccaggtcttc gaagggcaga tgcctcaact
    21061 gttgcatcgc ctcatcgggc gagagttcgc ctttcttcac ctgctcaaag agtatcttga
    21121 tggcttccga tttcattggg acacgcagcg tcgaacgccg cactgcgaga ttatcatttg
    21181 ctggccgaag cgcgttaaat gtgctgcagg acagcccgcg ccggagtgtt acgataaaaa
    21241 gctggcatgc ctaccgcaga aaaatcggcc ccacgcacgc tgacggtcgc cgttaccggt
    21301 gccagcggat ccatcttcgt caagcacctg ctcgacgcat tggatcgcga cgatcgtgtg
    21361 ggcacggtca acctaatctt ctccgatagt ggtctgcgcg tcatggccga ggagttggat
    21421 ggcgcgggcc gcagtgacgt agtgggtaag ctattgggcc ggccgtcgca gaagatcgcc
    21481 caacagaata atgccgacat cggtgccaac gtcgccagcg gttcctatcg caccgacgcg
    21541 atgattgtcc tgccgtgtag catgggcacc ctggcccgta tcgctaacgg ccttgccctc
    21601 cagttgatcg aacgcgccgc cgacgtctgc ctcaaagagc aacggccatt gatcctgtgt
    21661 gtgcgcgaga ctcccttcaa ccgcatccat cttcgcaaca tgaccctggc agccgaggct
    21721 ggagcaacca tctttccagt catccccgcg ttctatttcc gccccgccac caccgacgag
    21781 atcgcccgcg agtacgtgaa ccgcgtgctg gcgcatgtcg gattgccgca gcccgacgcc
    21841 tatcagtgga aagcaaaata atcgggaaat tcgcgtcaat gctctaaaac
//
LOCUS       DASRKK010000692         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1242470, whole genome shotgun sequence.
ACCESSION   DASRKK010000692 DASRKK010000000
VERSION     DASRKK010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242470"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFC15_16725"
     CDS             <1..413
                     /locus_tag="VFC15_16725"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZM11854.1"
                     /translation="EHRVYPLQERPILRGYEEFADLPENRLIQVYSLAEIATEKTIAL
                     ADQARNEPRDLYDLWHLTSSEGVELGDLLDAVRQKLEFRGKPFEGIAEAIRVKEGLLR
                     TLWSRRLAYQMTILPEFDEVFRAVQRTLRQAGVP"
     gene            682..2088
                     /locus_tag="VFC15_16730"
     CDS             682..2088
                     /locus_tag="VFC15_16730"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZM11855.1"
                     /translation="MRLLPHQIAFVETFFNPASKRVILLRGEAGLGKSGALVALASKF
                     LRERPTARALFVVPGAALRAHFVDKLRDAGVPTLSVDRYQFREMLDSAPGKQLWPAGV
                     VAVLSREFARQEDIRDALAAARWDLLIVDEAHQFRGTLAGKVLRQVGEASDRIVLATL
                     PNLELPDGFPEAEATVVQWRRDQVVDHDGALLDAIPRPILHEVPFSLSPAELSLAETV
                     GEVCRMFEGGTTQQRWVARSLLRSLQSSPAALEGALQRIAEIRNRVAHGMEPPPESTD
                     DDSLEDQLCGWLDPPTADKVARIAARALSAIEAVGSDSKLVAFGALLSRLSEVRTPFT
                     RITVVTEYLGTLFYLAAEIESLGQTCYVFHGGMNAESRQSTLALFSSGGGILTATRAT
                     MSEGVALSEVTDLVLYDVPGREVALRQVLGRFDRFGRRTQLNIYALLPTNSAEGSISE
                     SRGLLRQTFGWPVPDAHK"
     gene            2580..>2784
                     /locus_tag="VFC15_16735"
     CDS             2580..>2784
                     /locus_tag="VFC15_16735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM11856.1"
                     /translation="MGAGLEVDLKPGGPMNWVGTGADGKRSTYVRGEVLQSEPPKLLR
                     YTFALGSSSKTSRITIELVPESEA"
BASE COUNT          586 a          766 c          842 g          590 t
ORIGIN      
        1 gcgagcatcg ggtttatccg ctccaggagc gccccattct tcgcggctac gaggaattcg
       61 cggaccttcc cgaaaaccgg ctgattcagg tgtattcgct tgcagaaatc gcgaccgaga
      121 aaacgattgc ccttgccgat caggcacgca acgaaccgcg cgacctttac gacttgtggc
      181 atcttacgtc gagcgaaggc gtcgagcttg gggatctctt ggacgccgtg aggcagaaac
      241 tggaatttcg cgggaagcct tttgagggta tcgcagaagc catccgcgtg aaagagggtc
      301 tgctaagaac gctgtggtca cgccgcttgg catatcagat gacgatcctg ccggaattcg
      361 acgaagtatt ccgagcggtg caacgaaccc ttcgtcaggc tggcgttccc taggcgttcc
      421 ttgaatccgt tactgactgc gcgcatagtc ggggtcttgc gccagttcgt cctcggtaca
      481 atgtcgcggg atatcgcgcg cggggatcgg cgccattcgt aacgatctat ttcatttcaa
      541 gcgagaaatc acaatgcaag accatcagac tctcgctgta caccgtaact ggctgctgaa
      601 caagattaag caggcggaag gacatcgcaa gatggaggtg cggccgtgaa tccgacctct
      661 aacgaaccga aaaactcctc catgagactg ctacctcatc agatcgcatt cgtagaaaca
      721 ttcttcaatc cagccagcaa gcgtgtcatt ttgttgcgcg gagaagcagg acttggaaag
      781 agcggtgcct tggtcgctct cgcgagcaag tttctgcgag aacggccgac ggcgcgggcg
      841 ctgttcgtag ttcctggagc cgccctgcgg gcgcactttg tagacaagct gcgcgatgca
      901 ggtgtcccga ccctgtccgt ggatcggtat cagtttcgag agatgctcga ctctgcaccc
      961 gggaaacaac tatggcctgc cggagtcgtc gccgtcttga gccgggagtt tgccaggcag
     1021 gaggacatcc gggacgcttt ggcggcggcg cggtgggatc tcttgattgt ggacgaggcg
     1081 catcagttca ggggaactct cgcgggaaag gtcttacgac aggtcggtga agcatcggac
     1141 cgaattgtcc tagcaaccct accaaacctg gaattgcccg atggttttcc ggaagccgaa
     1201 gctactgttg tgcaatggcg gcgcgaccag gtcgtcgacc acgacggcgc gctgctcgac
     1261 gcgataccgc ggccgatctt gcatgaagtt cccttcagtc tgtctccggc cgagttgagc
     1321 ctggcagaga ctgtaggcga ggtgtgtcgg atgttcgagg gggggacaac gcaacagcgt
     1381 tgggtcgcga ggtcattgtt gcgcagcctt cagtcaagtc cagcagcgct cgaaggcgcg
     1441 ctacagagaa tcgcggaaat cagaaatcga gtcgcgcacg gcatggaacc gccgccggaa
     1501 tcgacggacg acgattcgct tgaggaccag ttatgcggct ggctggatcc tcccactgcc
     1561 gacaaggtgg ctagaatcgc tgctcgtgcg ctttcagcga tagaggcagt gggtagcgat
     1621 tcgaagttgg tagcctttgg tgcgttgttg agtcgactca gtgaagtgag gacaccgttt
     1681 acacgtatca ctgtggtcac tgagtactta ggcacgctct tctacttggc tgccgagatc
     1741 gagagcctcg gccagacctg ctacgttttc catggaggta tgaacgctga aagtcgtcag
     1801 agcaccctgg ctctgttctc cagcggcgga gggatcttga cggcgacaag agcaacgatg
     1861 tccgagggtg ttgcgttgag tgaggtaacg gatcttgttc tatatgacgt tcccggcagg
     1921 gaggttgcgc tacggcaagt cttaggtcgg ttcgaccggt tcggcagacg aacccagttg
     1981 aacatctacg ccttgctgcc cacaaacagc gccgagggct caatttctga gtcccgcggg
     2041 cttctccgcc agactttcgg ctggccagtg ccggatgcgc acaaatagac gagaacattt
     2101 cgttgtatct ggtttcgcaa ccgacggtta gcttccgcgt tgtcaagtag gccgtcttcg
     2161 gcgataaaag ggggcggctt ctgttactcc atgcgaatcg ttataaggcc actgactccc
     2221 cattcgtcga tccaagtatc gctcggagcg cgccgctgtg ccagcctgcc cgaaatcgtg
     2281 tgtttgtggg cacgtgaacc gcttgatcta tggggtcaga atatcagcca cttagcggaa
     2341 cggattcgcg ggggtaattg ggcaagcggg agaatgcaag gagcgctacc taactaacac
     2401 cacacttcct cgaaccagac ttctggacaa tcttcgggaa gacggactcg ccgaacacga
     2461 acagcgaagt agatcaccgt ggccgagcaa actccattgc acttcgtttt ctacttcgcc
     2521 gcaccccgga taaagtctgg gagggcttcg tctccccggg atccaaccgc attatctttg
     2581 tgggggccgg actcgaagtc gatctgaagc ccggcggtcc catgaactgg gtcggcactg
     2641 gggccgacgg caagcgcagt acctacgttc gcggcgaggt gctccagtct gagccgccaa
     2701 agttgttgcg atacacattc gccctcggct cttccagcaa gacctcccgc atcacgatag
     2761 aactcgtgcc ggagagtgag gcca
//
LOCUS       DASRKK010000693         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_656434, whole genome shotgun sequence.
ACCESSION   DASRKK010000693 DASRKK010000000
VERSION     DASRKK010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656434"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFC15_16740"
     CDS             <1..183
                     /locus_tag="VFC15_16740"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM11857.1"
                     /translation="DVNKFEAQLKALGKTVEIHIFPDAGHAFENPDNKQGYRADDAAQ
                     AWKLTVDFLAKNLKSK"
     gene            180..362
                     /locus_tag="VFC15_16745"
     CDS             180..362
                     /locus_tag="VFC15_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11858.1"
                     /translation="MTMLNRRVRYRKRLLWMLVAMVVASALWVLKVEIAYERNVRHIP
                     TQFRLRTHGRPYNWKA"
     gene            complement(687..1892)
                     /gene="pcaF"
                     /locus_tag="VFC15_16750"
     CDS             complement(687..1892)
                     /gene="pcaF"
                     /locus_tag="VFC15_16750"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZM11859.1"
                     /translation="MSNAFICDAIRTPFGRYGGTLSSVRTDDLAAIPLKALAQRNSSV
                     DWSALDDVILGCANQSGEDNRNVARMALLLAGFPQSVPGTTMNRLCGSSMDAVSVAAR
                     AIKTGENSLAIAGGVESMSRAPFVMAKSDAAFSRSVKVEDTTIGWRFVNPLMKAQYGV
                     DSMPETAENVAEEFRISRQDQDAFAYRSQQRTAAAIKSGALAEEIVSVTISGRKGVPV
                     IVDQDEHPRPDTAIEALAALKPIVKPDGTITAGNAAGINDGACALLLASEQAVNRYGL
                     TPRARVVASAAAGVAPRIMGMGPAPATRKVLALAKLSISDMDAIELNEAFASQALAVL
                     RDLGIADDAPHVNPNGGAISIGHPLGASGARLITTAVLQLQRTGGRYALCTMCIGVGQ
                     GIAMILERV"
BASE COUNT          451 a          665 c          700 g          465 t
ORIGIN      
        1 gatgtaaaca agtttgaggc acagctcaag gcgctgggca agacggttga gatccatatc
       61 ttccccgacg caggacacgc gttcgagaat ccagacaaca aacagggata tcgggctgat
      121 gacgcggccc aagcctggaa gctgaccgtc gacttcctgg cgaagaacct gaagtcgaaa
      181 tgacgatgtt gaatcgacgg gtgcgatacc ggaagcgcct gctgtggatg ctggtggcga
      241 tggttgtcgc gtcggccctg tgggtgctga aggtggagat tgcctacgag cgaaacgtga
      301 gacatatccc tacgcagttc cggttgcgga cgcacgggag accgtacaac tggaaagcgt
      361 agagccgttt ggctctgttg acatcccgct ttgagacagt cacccctgtc acaacaactt
      421 tcttgtgcgt tggcttagca gcggcgcgcc tttaggcgcg ccgctgcgtg tcccttattt
      481 ttgtcatcct gaaccggctt cagccggcga gggatctgct ttgggggctt tttcaacagc
      541 ctctttagcc caccgaggga actcacttcc ggacctgcaa aattctagtt ccctcagggg
      601 ctaaagccca gcttcaaaat gggcgatagg cgcagctgaa gctgcgcccc ttcaagagca
      661 cttgtgaaac aagctgtagc agtggactac acgcgttcca ggatcatggc gatgccctgg
      721 ccgacgccga tgcacatggt gcacaaggcg tagcgcccgc cggtgcgttg cagttgcagc
      781 accgcggtcg tgattagccg ggctccgctg gcgcccagag gatggccgat cgaaatcgct
      841 ccgccatttg ggtttacgtg cggagcgtca tctgcaattc ccaggtcgcg aagcacggcg
      901 agcgcctgcg aggcgaatgc ctcgttcaat tcgatcgcgt ccatatccga gatgctgagt
      961 ttcgccagcg caagcacttt gcgcgtagct ggagccggac ccatccccat aatgcgcggc
     1021 gccacacccg cagccgcgga ggcaaccacg cgcgccctcg gggtaagacc gtagcgattc
     1081 acggcttgct cggaggccag caacagcgcg cacgcgccat cgttaatgcc agcggcgttt
     1141 cctgccgtga tggtgccgtc aggtttcacg atcggcttca gcgcagccag tgcttcgata
     1201 gctgtatccg gacgtggatg ctcatcctgg tctacgatga cgggcacacc tttgcgtcca
     1261 gaaatcgtga ccgagacaat ctcttccgca agagcgccac tcttgatggc ggccgctgtg
     1321 cgctgctggc tgcgataagc aaatgcgtcc tggtcctgtc gcgagatacg gaactcctca
     1381 gcgacgttct cggcagtttc cggcatggag tccacgccgt actgggcttt catcagcgga
     1441 ttcacgaagc gccagccgat ggtggtgtct tccaccttta cgctgcggga gaaagcagca
     1501 tcggactttg ccatgacgaa cggcgcccgc gacatgctct ccaccccgcc ggcaatcgcc
     1561 agcgaattct ctccggtctt gatggctcgc gcggcgacgg aaacggcgtc catgctggag
     1621 ccgcataacc gattcatggt ggtgccggga acgctttgcg gaaagccggc gagcaacagc
     1681 gccatgcgtg cgacgttgcg gttgtcttcg ccggactgat tggcgcatcc cagaattacg
     1741 tcgtcaagcg cactccaatc cacggaggag ttgcgctggg ccagggcctt gagcggaatc
     1801 gccgcgagat cgtcggtgcg gacagaggag agcgtcccgc cgtagcggcc gaagggggtc
     1861 cggattgcgt cacagatgaa ggcattcgac attcggtaag acctgtgtgt tcaaccatga
     1921 taacttgaac gaatgtgctg gcgatttgcc gggatgagat aacgggaagg gaagcacgca
     1981 aaaccggcgc gtgatggggc tcccggctgc gcggctggat ggctgcggtt cattccatcg
     2041 tgcgtcgccc acgttagttt acacagactc aggcagggtg gctctctagt gtccaagtgc
     2101 ttccccaagg tttgaactgg gtgtaagctt cttactcccg cgtgcaagcg gactaacgaa
     2161 tcgtgctaag caacattctc atcatcatcg gctgcgtggt tgcggcgacc ctgtttctgt
     2221 tcctggtgac tcgtacctcc gcggcgcatg cgcgcaagga gtcaaacgac tttaccggcg
     2281 c
//
LOCUS       DASRKK010000694        24022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1144851, whole genome shotgun sequence.
ACCESSION   DASRKK010000694 DASRKK010000000
VERSION     DASRKK010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 24022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24022
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144851"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFC15_16755"
     CDS             complement(<1..375)
                     /locus_tag="VFC15_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZM11860.1"
                     /translation="MEPFRHHVFVCTQQKPEGVTCCPASGSMSVLGALHGELGKQGLS
                     NDVQVSTCGCLGLCDDGPIMIVYPEGVWYRQLKAGDVPEIVASHLKAGTAISRLVWND
                     PPAMKAAATEHTQHYLAMVKAKD"
     gene            653..2362
                     /locus_tag="VFC15_16760"
     CDS             653..2362
                     /locus_tag="VFC15_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM11861.1"
                     /translation="MRKTLALLALAAACSLGVAVAQDSNSLPPAPFKRVLLISIDGMH
                     AVDFLNCANGISTANNNQPYCPAIAALGQTGINYVSASTSKPSDSFPGLTAIVTGGSP
                     ALTGVYYDVAYSRDYSAPAKTTGNGLAAGPCTPYGPPTGTTTEYEEGIDINQQKLNGG
                     APGASLTDGGLASIDTKRLVRDPGNGCAPVMPWEFVRANSIFSVIHQAGGYAAWSDKH
                     PAYASVASGIGPSALDDFYAPEINSNVIGLPKVKTPTGISCATIRDKGADLTAWTNSF
                     ENIQCYDTLKVDAILNEIDGKDHLGTKTTKVPTIFGMNFQAVSVGQKLIEKSNGVTGG
                     YLDAAATPSAALLNEFQFVDASIGAFVKELNKQGLLSSTLIVITAKHGQSPIDPSRYV
                     GQTINGTSPATLLSNDGYLPYSESTNNPTGIGPTEDDVSLIWLNSAFDTDAAVAVLEA
                     NASATTGIALGQIYYGPSLFLNYNDPTTDPRTPDIIVTPNVGVTYSGSHAKQAEHGGF
                     AHDDTNVVLLLSNPSLKPRTVSAGVGTAQVAPTILKALGIKPGTLDAVRAEGTGVLPT
                     IPF"
     gene            2497..4665
                     /locus_tag="VFC15_16765"
     CDS             2497..4665
                     /locus_tag="VFC15_16765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZM11862.1"
                     /translation="MKRTFAALLVFTLNLSPIAHGQQPAEKLPFNPPTTISGYQNSAA
                     ELQAEKAFLAVPSAELAKEHLRILTSAPHVAGSPEDKKTAEYVLQKYKEAGLDAYIQE
                     YKIWMNTPAEIKVDVVAPEGVMMHGPTREHVSDDPYQDDLRVLTAYNAFSPSGDVTAD
                     VIYANYGRPEDFKKLKELGVEAKGKIAIVRYGANFRGVKPFVASEYGCVGVIIYSDPW
                     DDGYYKGDKYPKGPWRPDTAVQRGSVQYLFRYPGDPTTPGIASVPDLPDSKRTPLDQV
                     TDLTKIPTTPLSYADASPILANLGGPESPREWQGALPFTYHVGAGPVKVHMLLKQNYQ
                     WTTIYDVIGMVKGSEFPQQWVITGNHRDAWVYGAVDPNSGTAAQLEAVHGLGELLKTG
                     WKPKRTIVFASWDAEEEGLIGSTEFAEQHADELRQAVAYFNMDVAVAGPDFGASSVPS
                     LKQYLREISKAVPSPKGGSVYEQWKATTEKRDKEKAKSTQAGAASREPVVVTKQDVRV
                     GDLGSGSDYTPFLQHLGVPSADIGSSGPYGVYHSVFDNFAWFTKFGDPTFAYEQQMAR
                     VYGLDTIRMASEDVLPFNYEDYGKEIGEYVKAADQKSKDTFGAKSPSFADAQKAASRL
                     EKAGAQMLLTQSGMKGNTARVNQALRDTERAFLIDGLPDRPWFKHSIYAPGKYTGYAA
                     VVIPGVNEAIDASDSALTLEQLKVLTDALNRAAETLEKGM"
     gene            4853..5548
                     /locus_tag="VFC15_16770"
     CDS             4853..5548
                     /locus_tag="VFC15_16770"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZM11863.1"
                     /translation="MKTAGMIGGLGPESTIDYYRSILARYRARKPGGSYPQIVINSLD
                     VEKGIAMLDAGRLDDLADYLVCGIETLVRAGADFAFIAANTPHLVFDELQRRSAIPLL
                     SIVRTTADRAKALCLKKVGLFGTGFTMRASFYPEEFQGAAIVLVTPTESEREFIHKKY
                     IGELLKNQFLPQTRADIMRIAQRMKDEDGVEAIVLAGTELPLLLREAANQGIEFLDTT
                     VIHVEAVVEELLR"
     gene            5717..6439
                     /locus_tag="VFC15_16775"
     CDS             5717..6439
                     /locus_tag="VFC15_16775"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZM11864.1"
                     /translation="MLPDSEPPRHAAVVRVTHWVTAICFFALLVSGLEIVVSHPRFYW
                     GETGNVNTRPLFRIPIPASRSMVPTGYGYVLPDQNGWSRYLHFQAAWALVLTGLAYCI
                     WGVWTGHFRRNLFPAPADRTWQAFVAQMANHLRLRPPGAAEAYSYNVLQRAAYLAVIF
                     VVFPLVIWTGLALSPGFNSAVPAGVNLLGGRQSARTLHFFLTGFLLAFLVVHVGMVIW
                     AGFTSRVRAMVTGVATAPEGRT"
     gene            6436..7194
                     /locus_tag="VFC15_16780"
     CDS             6436..7194
                     /locus_tag="VFC15_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM11865.1"
                     /translation="MTKISRRKFVTTGLAVAAGASGLAVAAGLAKRYGLVPPDSGGIY
                     GPGETLTYAAQRLMTTHSLAREFPRSMISKTPFANELEPFNDSFKSHQANGFADWRLA
                     VDGMVARPASLSVSDLKSFPVGSQITEVVCEEGWSYVAEWIGTPLHEVMNACGVLPQA
                     KYVVYFSIEPDWWESIDMADALHPQTLLTWGMNDGELPVGFGGPLRLRVPRQLGYKSV
                     KFITRLTFTDSLKGFGKGLGSASPEGGYAWYAGI"
     gene            7510..8526
                     /locus_tag="VFC15_16785"
     CDS             7510..8526
                     /locus_tag="VFC15_16785"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZM11866.1"
                     /translation="MSNHFTGLSLGPPLGDQRLDLCDLYAFQSPTDPARTVLILNANP
                     NADALHPDAIYRLAIDNDGDYLNDIAFSYVFSPPQNGRQTVSVFVAKGTESRSVEAVG
                     TKIISDAAVSFVGDKPNIVKSEAYTFFAGARSDAFFFDFDGIKNLFDTRGKRNFTEPH
                     LGGKSPWTGVDSNTEANVFSTVLELPTSELGANPKLNIWGRCSVRRDGKLLHVDRAGH
                     PSVSSFFNTDDTKEEYNASEPVNDRKRWTDQFVHLMGHTGNYSREEAIAAIDVEGLLP
                     DMLSFNPSKPAKYPNGRVFTDDVIDYRLAFLSKGDIPPDGLKPHTDTLNEFPYLGTPH
                     PKKS"
     gene            complement(8728..9165)
                     /locus_tag="VFC15_16790"
     CDS             complement(8728..9165)
                     /locus_tag="VFC15_16790"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM11867.1"
                     /translation="MPVWLGYILLVAAWVHPFGMVVIPHTVIAEYLGVAICAVGLFAA
                     IWSRKTLGGDWSRDVELKQGHTLVERGPYRFVRHPIYTSHLLMGLGTAIASGLLVAFV
                     GLLLFFIGFWIKLKQEESLLLSYFPDEYPAYKARVKALVPFVF"
     gene            complement(9412..10302)
                     /locus_tag="VFC15_16795"
     CDS             complement(9412..10302)
                     /locus_tag="VFC15_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZM11868.1"
                     /translation="MGSSGQSPIFEVLFDRAEPGPFADDALAPYGNFGFPAPPADRPW
                     IYTNFVQSLDGIVSLLGKHASGAEIAQSRADRWLMDLLRAHADGVIMGMNTLREEQRN
                     RGPESRGIVFQVADPGLLQFRKKLGKSRQRNIFVTRALDLDLSQFKCFDGDAVDVAIV
                     TSPAGTERLSALKTHPHVTIIAPGTGESVDLSAAVRELRAQLGIKYLLCEGGPTLYGN
                     LARADLVDEKFLTVSPLEIGQEVPGEQERLPSEAREGPLLRPTVFAGPGFTRETMTRW
                     TWLSSRKSGDHQFNRYRRMR"
     gene            10357..11100
                     /locus_tag="VFC15_16800"
     CDS             10357..11100
                     /locus_tag="VFC15_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11869.1"
                     /translation="MMENFSQVLILADAGGSMGERGGANIEIAHKLNEAEEHGPHPKS
                     KWIETLEIAEAIILALVAIATAWSGYQAAKWDGRQDELYEQSTRLRVQAQGLATRAGQ
                     EQIYDASTVVEWIKAQSSGNKKLAELFERRFRPEAKVAFEVWKKLDPLNNPDALAGPL
                     LMPEYHNAHAEESAKLSQEATEAFEQGSKSRSTSDDYVRVTVLLATVLLLTAIAQRFK
                     THQVRVGLAVVAFLLVCIPIYRILNLPRL"
     gene            11135..11791
                     /locus_tag="VFC15_16805"
     CDS             11135..11791
                     /locus_tag="VFC15_16805"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HZM11870.1"
                     /translation="MDIKLSDIEARVLGALVEKDITTPDYYPLSLNALVNACNQKNNR
                     EPVMNLEEGAVRDALNGLHDDGLAGPASGADSRVTKYEHRLQETFNFSRGETAVLCVL
                     LLRGPQTPGELRGRTERMHHFEDLDAVQSSLQKLMQREPPLAAVLPRQPGTKESRYAH
                     LLSGEVEAGELPAARAVPSSSASGGDERLSRLEEAVTALRNEVGELKQQFADFRKQFE
                     "
     gene            complement(11809..12984)
                     /gene="nrfD"
                     /locus_tag="VFC15_16810"
     CDS             complement(11809..12984)
                     /gene="nrfD"
                     /locus_tag="VFC15_16810"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZM11871.1"
                     /translation="MYPNEIELQWSVLIVLYPFLTGLVAGAFILASLERVFRVDAVKP
                     TYRLALLTALAFLLVAPLPLQLHLGHPERSLEMYLTPHTSSAMAMFGFVYMWYLMAVL
                     VLEVWLDYRRNIVVRSQTTTGWTRWIYKVLTLGSTNISERALAIDNKVGWIITIIGIP
                     SAFLLHGYVGFIFGSVKANPWWSSPLMPVVFIFSAMVSGIAVVMLIYMFMCRVRGQQI
                     DMRCVDTIAKYLFYTFLIDFALELLDLIHRIYESDESFRSLDFMVHTKLYFPHIVIQI
                     LLGTLTPILLLAATQVLPLNAARRRVTYGIAGCLTLIGIFAMRWNVVIGGQLFSKSFL
                     GYTTYKMAFVTREGLVVAIVLTILPFIILWGLVKLLPPWEETHSLDLSPVALAHGDD"
     gene            complement(13020..13751)
                     /locus_tag="VFC15_16815"
     CDS             complement(13020..13751)
                     /locus_tag="VFC15_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZM11872.1"
                     /translation="MTITRRRLLMLLPAAAIAWDSILAGTPEESPNYKMTDHWWGMLI
                     DVTKCIGCGTCVQACKAENNVPDGYYRTWVERYHVAKWDTEMPQVDSPDGALHGFPPT
                     KQEGGKNFFVPKLCNHCADSPCTQVCPVGATFVSPDGVVLVDQKYCLGCRYCIQACPY
                     GCRFLNPETETASKCTLCYHRITKGLTTACCETCPTGARQLVDFKNPNDPIHKFLETH
                     SVQVLKPHLATRAKVYYYGLDGSVR"
     gene            complement(13748..14221)
                     /locus_tag="VFC15_16820"
     CDS             complement(13748..14221)
                     /locus_tag="VFC15_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZM11873.1"
                     /translation="MANLKDAGHLFRMAAVFAIGIVLFLVLRAIFVPKTFGQYGHYRA
                     AAIAEVAARPVMFAGHQACESCHTDVLEVKSKGVHAHVACESCHGPLAKHADDPTALQ
                     PPHIDVAVLCVRCHEANIAKPKTFPQVVSADHSGGMVCDTCHKPHSPKIDSGVSQ"
     gene            14526..14921
                     /gene="msrB"
                     /locus_tag="VFC15_16825"
     CDS             14526..14921
                     /gene="msrB"
                     /locus_tag="VFC15_16825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZM11874.1"
                     /translation="MAEKIKKTEEEWRKELTPEQYRVLREKGTERPFTGDYEGVTTPG
                     VYKCAACGQELFLSDSKFDSGCGWPSFFQPAAEEKVDAHEDNTLGMRRVEVTCSHCGG
                     HLGHVFPDGPRPTGLRYCINSASLKLDPK"
     gene            complement(14976..15623)
                     /locus_tag="VFC15_16830"
     CDS             complement(14976..15623)
                     /locus_tag="VFC15_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11875.1"
                     /translation="MAVIVVGGHSRSVGKTSVVAGIIAALPEMHWTAFKITQYGHGIC
                     SANGEPCDCATDDHSLAVTEERDRSGESDSSRFLVSGAARSIWVRTRQGMLAEAMPRL
                     RKELASAENAILESNSVMRFLRPDLYLTVLDAANPDFKASAKEYLDRADAIILHDGPT
                     QPDWTKVSLKPVEGRPVFRIHPPPYVTPELIEFLRERLSQRGGSAAFRPASTSLL"
     gene            complement(15629..16630)
                     /gene="lpxD"
                     /locus_tag="VFC15_16835"
     CDS             complement(15629..16630)
                     /gene="lpxD"
                     /locus_tag="VFC15_16835"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZM11876.1"
                     /translation="MSQTLQSLAEFIAARLIGDGGIRIERVASIARANQGDLVFVESD
                     RHLDEALNSNASAIIAGEFAAGSTGRKPLLISSRPRLAFATAATMLYPRAKFTPAIHP
                     TAIIDQSAKVSPSSSIDAYAVIEADAVVGERCFIASGCYIGPGVVIGDDCEVYPRVVV
                     YPGTTIGRRVVVHAGAVLGSDGFGFVRDETYGRYVKFPQVGRLVIGDYVEIGANCTID
                     RGALDETVIGPGTKLDNMVHVGHNCSIGANVVIAAQTGISGSSSVGDDCLLGGQVGLG
                     DHVTIESGAILGGQSGVLPNKTIRGKGIVYWGTPVKPVREYLKALATLSRLTRKAGK"
     gene            complement(16843..17742)
                     /locus_tag="VFC15_16840"
     CDS             complement(16843..17742)
                     /locus_tag="VFC15_16840"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM11877.1"
                     /translation="MLYRLQYAPLFLLVRFIGVLPRPVARGVGLVIGGIVYHLHPRLR
                     RVGMRNLELAFPHKPVPERRRILRGVYRSLGRLLAEFCLFPRYTPDNVSKVAVYQGFE
                     NFDAAEKRGKGVLFLTGHFGGWEIGSFFHSLQGHPMRIVVRPIDNPYVDDLVTRYRTL
                     HGNTVIGKQGFARGLLAAMENNETVGILMDTNMMLPQGVFANFFGIPACTAVGIARVA
                     LHTDAAVVPAFTIWDPVLRKYRVEFDRAIELVRTGDNEADAVTNTAAFNRVFEDYVRK
                     YPDQWLWVHRRWKTRPPGEPPLY"
     gene            complement(17864..18730)
                     /locus_tag="VFC15_16845"
     CDS             complement(17864..18730)
                     /locus_tag="VFC15_16845"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM11878.1"
                     /translation="MKKISVALLVLVSTAMWAQSAARSADVERVQSATTILSQIMEAP
                     DRGIPDSIMSGAKCIAIVPSMMKISFILGANYGKGVATCRTATGWSSPAFFRIEGGSF
                     GFQAGGQASDLVLIIRTDDGMQHLLQSKFKLGADASAAAGPVGRDAQAATDLTMRAQV
                     LTYSRSRGLFLGVSLSGAVIRQDDGDVRAFYGKDWTFYSLLNGEVPPPPDSQAFLNSI
                     EHYAPTRRAATPAVAPASAAAPTPAPAATAEPTASATPATEPAKTPAEAPAATPAPAT
                     TTDPAGSGNNLR"
     gene            complement(19109..19768)
                     /locus_tag="VFC15_16850"
     CDS             complement(19109..19768)
                     /locus_tag="VFC15_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM11879.1"
                     /translation="MKSAVKNPIRIALVENDPLRSVGFRALFDADPDFEVQSCTSTSI
                     FGNRNFDLVLIGSRTGPAMYEVMAALKALDPSVRIIATGNVPGDEMALRALCAGAKGY
                     VEESAPVAEFKKAVREVHSGSVWISRRLFGMFIERVTSVLRSTARQEPLVFTEREREV
                     LHLLLDGRSNREIGHELGIEERTVKAHVAKLMRKAGVTNRIALSVHALTHSLLGVGQS
                     E"
     gene            19982..20722
                     /locus_tag="VFC15_16855"
     CDS             19982..20722
                     /locus_tag="VFC15_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HZM11880.1"
                     /translation="MLSFDQSSALPAAVVAIGFAMLARFMGAVSDGGALVGIVIAFLV
                     MVAAGMGGLLPLLAVLVMTLLATRWRNERKRSMGVSERGSGRTASQVAANLGATALCA
                     AAVIVFPRHSGIFMVGATAALAEAAADTVSSEAGQAAAKQARMIVNFQPAPIGTNGAV
                     SLEGTLAGCMAAFIVAWVSSFFGVVAGQWAPVVAVAGIAGMFFDSVLGATLENRGKMG
                     NDSVNFVSTVFAADLAILVILFVEGVSG"
     gene            20789..22762
                     /locus_tag="VFC15_16860"
     CDS             20789..22762
                     /locus_tag="VFC15_16860"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM11881.1"
                     /translation="MARIKLMLFLLCAVAAAPLLAQQYDVLIRNGRVIDGSGNPWIYA
                     DVGIIGDRVAFLGHAGTGVTAKRTIDATGLIVAPGFIDMLGQSEFNLLVDKQAVSKLT
                     QGVTTEITGEGESIAPTNDALNAEHADVLDHYHVTGDWRSLEEYFQRLTKQGSGINLG
                     TYVGAAQIRRLVIGTSDRPPTPEELKDMEIAVDDAMSDGAMGVSSALIYAPGSYAKTE
                     ELIALAKAAAAKGGVYASHIRNEGDSETEALNEAFRIGREANIPVQIFHLKISGKQNW
                     GKMPQVIDEIEQARVSGVDVTANQYPYIASATSLGAIIPPKFHDGGTDAFVARLKDPA
                     TRALIRTDLEGGSGAFENMWRGTGGPTGVMVVSVLNPEYRKFEGKTIAQIAQMENKDP
                     LDAALDLIVADRDNVGAVYFSMSEEDVKLAMRQPWVSVGTDYGEVSPTGPLGESKSHP
                     RAYGSFARILGKYVREEHNLRMEDAIRKFTSLPAQQVKLENRGLLRSGYFADVTVFNP
                     ETVKDMATFEDPNRTSVGFEYVFVNGVLSLEHDKVTGQMGGRPLRGPGYMMRDYVPDG
                     LPVRGKVQGVVTDEGGYPLPRSTVTLTDASGKVLGTSNIKKDGRYEIVLEQPCHGCSL
                     KSERAGFVMQTKTGIEYNGSNSLWFGFALKRSN"
     gene            22953..23744
                     /locus_tag="VFC15_16865"
     CDS             22953..23744
                     /locus_tag="VFC15_16865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZM11882.1"
                     /translation="MIKWKTFTIGWSLALALLLFAAHKTTMPDQSSYRDVVDLTHNLN
                     DHSPNWEGSEQSPFHAKQLGDIEHDGYYSRIFTTQEHYGTHLDAPAHFARGMWTVDQI
                     PAERLVRPLVVLNVRGKANDNPDYEISVQDIADWETQNGEIPAGAVVMAYTGWEDRWS
                     APKAFRNAGADGVPHFPGYSLEAVTFLVKTRNTVGLGIDTMSMDPGASKTYPVHQFTA
                     KESVYQLENVANLGLVPAAGATIVVAPIKLEDGSGGPARLLALVK"
     gene            complement(23825..>24022)
                     /locus_tag="VFC15_16870"
     CDS             complement(23825..>24022)
                     /locus_tag="VFC15_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11883.1"
                     /translation="VELLLQTEVLVIENEQEVFSAMIALHLGRGDFADALIAALGAKA
                     GCQHTLTFDQKALRLAGFQPA"
BASE COUNT         5092 a         6752 c         7435 g         4743 t
ORIGIN      
        1 gtctttcgcc ttcaccatgg cgagatagtg ctgggtgtgc tcggtcgctg cggctttcat
       61 ggcaggaggg tcgttccaca ccaggcggga tatcgccgtg ccggccttca agtgagaggc
      121 cacgatctcg ggaacgtcgc cggctttgag ctggcgatac cagacgcctt ccggatacac
      181 gatcatgatg ggcccgtcat cgcacaggcc gaggcagccg caggtgctga cctgaacatc
      241 gttgctcagt ccctgcttgc ccagttcacc gtgcaaggct ccaaggacgc tcatcgatcc
      301 gctggccgga cagcaggtga ctccctcggg cttctgttga gtgcagacga agacatgatg
      361 acggaacggc tccattgatt attctcctgt ctttgattgt ggtgtgagat ggcaaaagaa
      421 gccgtgatgc gggtcacatc gcgcaggcag gccaggcggc tcggcacagt aaactctcgc
      481 gatgcatctc ggttcttaag cacattacga ttcgcttccc tgattgtcat cgcagtttaa
      541 tactccgacg agaattcatc gcaatttcat tccagccccc gctgcgcgtg gctatgatcg
      601 caacaagtcc acgcctcctt cgtcgaaacc aatccaatct ttgaggtcca caatgagaaa
      661 gacacttgct ctacttgccc ttgcggctgc ctgttcgctg ggcgtcgcgg tcgcccaaga
      721 cagcaactca ctgccgccgg cccccttcaa gcgcgttctg ctgatcagca tcgacggtat
      781 gcacgctgtt gatttcctga actgcgccaa tggcatcagc accgccaaca acaaccagcc
      841 ctattgcccg gcaatagctg cgctgggcca gactgggatc aactacgtct ccgccagcac
      901 ctcaaaaccc tccgattcct ttcccggcct gacggccatt gtcaccggtg gaagccctgc
      961 cctgaccggc gtctattacg acgtcgctta ctccagggat tacagcgcgc ccgcgaaaac
     1021 taccggcaac ggccttgcgg ccggcccctg caccccctat gggccgccaa cgggtaccac
     1081 aactgagtat gaagaaggca tcgatatcaa ccagcaaaag ctgaacggcg gcgcacccgg
     1141 cgcgtcgctg actgacggcg gcctcgcctc catcgatacc aagagactgg ttcgcgaccc
     1201 cgggaatggc tgcgcgcccg tcatgccatg ggagttcgtt cgcgccaaca gtatcttcag
     1261 cgtgattcac caggccggcg gatacgcggc gtggtcagat aagcaccctg cctacgcctc
     1321 cgtcgcctcg ggcatcggac cgagtgcgct cgacgatttc tacgcacctg aaatcaactc
     1381 caacgtgatc ggtcttccca aagtcaaaac gcccacaggc atatcctgcg ccacaatccg
     1441 cgacaaaggc gccgacctga ccgcttggac caacagcttc gagaacatcc agtgctacga
     1501 cactttgaag gttgacgcga tcctcaacga gatcgatggc aaggaccacc tcggcaccaa
     1561 gaccaccaag gttcccacca tcttcggcat gaacttccag gctgtgagcg tgggccagaa
     1621 gctcattgag aagagcaacg gcgtgacggg cggatatctg gatgccgcgg ccacacccag
     1681 cgcggccctg ctgaacgaat tccagttcgt cgacgcctca attggggcat tcgtcaaaga
     1741 gctcaacaaa caggggctgc ttagttcgac cctgatcgtg atcacggcca aacacggcca
     1801 gtcgcccatc gatcccagcc gctacgtcgg tcagaccatc aatggaactt cacccgccac
     1861 cttgctgtcc aatgacggct accttccata ttccgaatct accaacaacc ccaccggcat
     1921 tggcccgacg gaagatgacg tctcgctaat ctggctgaac agcgcgttcg atactgacgc
     1981 cgccgtcgca gtcctcgaag ccaacgcttc cgccactacc ggaatcgcgc tcgggcagat
     2041 ttattacggc ccttcgcttt tccttaacta caacgaccca accacagacc cccgcacgcc
     2101 ggatatcatc gtgaccccga atgtaggcgt tacgtattcc ggcagccacg cgaaacaggc
     2161 cgagcacggc gggttcgccc atgacgacac gaatgtcgtt ctgctgctgt ccaatccgag
     2221 cctcaaacct aggacagtga gcgccggagt gggtactgcc caggtggcgc ctaccattct
     2281 caaagccctg ggtatcaagc ctggcaccct ggatgcagtc cgtgcggaag gaacgggcgt
     2341 ccttcccacg attccattct aaccagtccc aaaagctcgg ttcagggcgc ggttggcaat
     2401 tcagaaacca ttgcatttcc gaatcgtcct ccgcgccaca atttcggatt cttcgggtaa
     2461 tctagatgcc aaccgtactc tgaggagagt tcccgattga aacgaacgtt tgctgccctt
     2521 ttggtcttta ccctcaacct ttcgcccatc gcccacggtc aacaaccagc cgagaagctt
     2581 ccatttaatc caccaacgac gatcagcggg tatcagaatt ccgccgcgga actgcaggcg
     2641 gagaaggcgt ttctcgccgt gcctagcgcg gagttggcga aagagcactt gcgcatcctg
     2701 acctcggcgc cgcacgtggc ggggtccccg gaagataaga agacggctga gtatgtgttg
     2761 cagaaataca aggaggctgg gctcgatgcc tacatccagg aatacaagat ctggatgaac
     2821 actccggcgg aaatcaaggt cgatgtggtc gcgcccgagg gcgtcatgat gcatggtccc
     2881 acgcgcgagc acgtgagcga cgatccctat caggacgatc ttcgtgtgct gacggcgtac
     2941 aacgcattct cgccgtctgg cgatgtgacg gccgatgtca tctatgcgaa ctatgggcgt
     3001 cctgaagact tcaagaagct gaaggagtta ggcgtcgagg cgaaagggaa gatcgcgatc
     3061 gttcggtacg gtgcgaattt ccgcggagtg aaaccgttcg tggcctcgga gtatggatgt
     3121 gtcggcgtga tcatctactc cgatccatgg gacgacgggt actacaaggg agacaagtat
     3181 cccaagggac cttggcggcc cgacactgcg gtgcagcgcg gctcggtgca gtatctgttt
     3241 cgctatcctg gcgatcctac gacgcccggg attgcgtcgg ttccggatct gccggacagc
     3301 aagcgcacgc cgctcgatca ggtgacagac ctgacgaaga ttccgacgac tcccttgtcg
     3361 tatgccgatg cgtcgccgat cctggccaac cttggcggac cggaatcgcc gcgcgagtgg
     3421 caaggagcgt tgcccttcac ctaccacgtt ggcgcggggc cggtgaaggt acacatgctg
     3481 ctgaagcaga attaccagtg gaccaccatc tatgacgtga ttggcatggt gaagggcagt
     3541 gagtttcccc agcagtgggt gattacaggg aatcatcgcg atgcgtgggt gtatggggcg
     3601 gtcgatccta atagcgggac ggcggcgcaa ctcgaggcag tgcacggact gggcgagttg
     3661 ctgaagaccg ggtggaagcc gaagcgcacg attgtcttcg ccagttggga cgcggaagaa
     3721 gagggactga tcggctcgac tgagtttgcc gagcagcatg cggacgaact tcggcaggcg
     3781 gttgcgtact tcaacatgga tgttgccgtc gccggtccgg attttggggc gtcgtcggtg
     3841 ccaagtttga agcagtacct gcgcgaaatc tcgaaggcgg tgccgagccc gaagggcggc
     3901 agcgtttacg agcagtggaa ggcgacgacg gagaagcgcg acaaggagaa ggctaagagt
     3961 acacaagcgg gcgctgcgag ccgcgagccg gtggttgtca ccaagcagga cgtgcgagtc
     4021 ggcgatctgg gtagcggctc agattacacg ccgttcctgc agcacctggg agttccgtcg
     4081 gctgacatcg gatcgagtgg gccttacggc gtgtaccact cggtgttcga caacttcgca
     4141 tggttcacca agtttggcga tccgacgttt gcctacgagc agcagatggc gcgggtgtat
     4201 ggactggaca ccattcgcat ggcatcggaa gacgtgttgc ccttcaacta tgaagactat
     4261 ggcaaggaga ttggcgagta cgtgaaagcg gcggatcaga aatcgaaaga cacctttggg
     4321 gcgaagtctc cgtcgtttgc ggacgcacag aaggcagcgt cgcggttgga aaaagcgggc
     4381 gcgcagatgc tgctaacgca gagcgggatg aaagggaaca ctgcaagagt taatcaggcg
     4441 ttgcgcgaca cggagcgggc attcctgatc gatgggctgc ccgaccggcc gtggttcaag
     4501 cactcgatct atgctccggg taaatatacg ggatatgcgg cggtggtgat tccgggagtc
     4561 aatgaagcga tcgacgccag cgactccgcg ttgacgcttg agcaattgaa ggtcttgaca
     4621 gacgcgctga atcgcgcggc cgagacgctg gagaagggaa tgtaagcagt tgtcagtgac
     4681 cacttgtcag ttggaatgcg tcgaggcatt caagctgact gtcctgcgct tccacattct
     4741 tttagcgata gggcgggcac ggctggaggc ctatttttta aggtattgga tacgtgaggc
     4801 ccgtgcctgg gtggatggaa ctgacaactg aaaaccgaca actgacaact gtatgaaaac
     4861 cgcaggcatg atcggcgggc tgggtcctga gtccaccatc gattactaca ggtccatctt
     4921 ggcgcgctat cgtgcgcgaa agccgggtgg cagctatccg cagatcgtca tcaacagcct
     4981 cgacgtcgag aagggcatcg ccatgctgga cgccgggcgg ctggatgacc tggcagatta
     5041 tcttgtctgc ggcattgaga cactggtgcg cgcaggcgcg gactttgcct ttatcgctgc
     5101 gaatactccg cacctcgtgt tcgatgaact ccagcggcgg tcagccatcc cgctcctcag
     5161 cattgtccgt accaccgctg accgggccaa ggcgctttgc ctgaagaagg tgggcctgtt
     5221 tggaaccgga ttcaccatgc gcgcgagctt ctacccggag gagtttcagg gggctgcgat
     5281 tgttctcgtg acacccacag agtcagagcg ggagttcatc cacaagaagt acattggcga
     5341 gctgctgaag aaccaattcc tcccgcagac gcgtgcggac atcatgcgca ttgctcaacg
     5401 catgaaagac gaagatgggg tggaggccat cgtcctggca gggacggaac ttcccctgct
     5461 gctgcgagag gctgccaatc agggcattga gtttctcgac acgaccgtga ttcacgttga
     5521 ggccgtggtg gaagagttgc ttcgctgacc tgattcgatt gaggaaagga cgccagatgc
     5581 gacatggccg cgcagaaacg gccatacgcg aggggggctc ccccaatcca cggacttcga
     5641 gatcaagaaa tacaccctaa ccgcctcgcc tcggccgcga cgatcgtgct atgctcccgc
     5701 agtgcgcgac gccggcatgc tccccgattc tgaacctcct cgccatgccg cggtcgtccg
     5761 cgtgacccat tgggtaaccg cgatttgctt ctttgccctg ctggtcagcg ggctggagat
     5821 tgtggtttct caccctcgtt tttattgggg cgaaaccggc aacgtgaata ctcgcccgct
     5881 gtttaggatt cccattccag cttctcgatc aatggttccc acaggctatg gctacgtgct
     5941 gccggaccag aatggctgga gccgctacct tcactttcaa gcggcctggg cactggtgct
     6001 gacgggtctc gcctactgca tctggggtgt atggaccggc cactttcgcc ggaacctgtt
     6061 tcccgctccg gcggaccgaa cctggcaggc gtttgtcgcc cagatggcca accaccttcg
     6121 tctcaggccg cctggcgcag ccgaagcgta ttcgtacaac gtgctgcagc gggccgcata
     6181 cctggcggtg atcttcgtcg tgtttccgct ggtgatctgg acgggactgg cgctgtcacc
     6241 cggcttcaat tccgcggttc cggcgggcgt caatctgctg ggagggcggc aatcggcgcg
     6301 cactctgcac ttctttctta ccggatttct cttggccttc ctcgtcgttc acgtgggcat
     6361 ggtgatttgg gccggattca ccagtcgcgt ccgggcgatg gtcactggag ttgccaccgc
     6421 gccagagggg cgaacatgac caagatctca cgccgcaagt tcgtcacgac gggactggca
     6481 gtcgccgcgg gcgcgtcagg cttggccgtg gctgccggcc tggcgaagcg ctacggactc
     6541 gtcccgccgg atagtggcgg aatctacggt ccgggcgaaa cgctgaccta tgcggcgcag
     6601 cgtctgatga caacccactc gctggcccgc gaatttcccc gtagcatgat ttcgaaaacg
     6661 ccgttcgcca acgaactcga gcccttcaac gactccttca agagccacca ggccaatggg
     6721 ttcgcagact ggcggcttgc tgtggatggc atggtcgcgc ggcccgcttc gctttcagtg
     6781 tctgacctca agagcttccc cgtcggcagc cagatcaccg aggtcgtgtg cgaggagggc
     6841 tggtcctatg ttgccgaatg gataggaacc cctttgcacg aggtcatgaa cgcctgcggg
     6901 gtactgcccc aggctaagta tgtcgtttat ttttccatcg aacccgactg gtgggaaagc
     6961 atcgacatgg ccgatgcgct gcatccccag actctgctga cttgggggat gaatgacggc
     7021 gaacttccgg tgggtttcgg ggggccgctg cggctgcgtg tgccgcggca actcgggtac
     7081 aaaagcgtga aattcattac ccgcctcacg ttcacggact cgctcaaagg cttcggcaag
     7141 ggactcggct cggcctcgcc ggagggcggt tatgcctggt atgcgggcat atagatcgct
     7201 ctgtgatcgt cgctgggaga ttattgtttc tttcttcgcg gttaactgac gatatggcct
     7261 gactacgtaa acaatcccag ggccgttgac tccgactcaa tcgccagcgc ttcgttgacc
     7321 cgaagtcggc ccaccagagg agcgacctag cggatcggca acagatttat ctttaatcct
     7381 tgcgaaacgg cgcggaagta aaacgggtgt atactcctca cgccggaatg ctaggaaacg
     7441 gatttcgggg gccacccttc tgtaggacac cacaatcgca gttccaaatt ttcaaaggca
     7501 ggtaagaaaa tgtcgaacca tttcactgga ctcagcctcg gtcctcctct cggggaccag
     7561 cgactcgacc tatgcgatct ctacgccttc caatcgccca cggacccggc acgaacggtg
     7621 ctcattctca atgcaaatcc gaatgccgac gcgctccatc ctgacgccat ttaccggctc
     7681 gcgatcgaca atgacgggga ttacttgaac gacatcgcgt tcagctatgt attctcgccg
     7741 ccgcaaaacg gcaggcagac cgtaagcgtc tttgtcgcta aaggcactga atcccgctcg
     7801 gtcgaagcgg tagggacgaa gatcatctcc gacgccgcgg tttcgttcgt cggcgacaag
     7861 cccaacatcg tcaagtcgga ggcgtacacg ttcttcgccg gcgctcgcag cgacgccttc
     7921 tttttcgact tcgacggtat caagaatctg ttcgataccc ggggcaaacg gaactttacc
     7981 gagcctcatc tgggcgggaa gtctccgtgg accggcgtgg attcgaatac ggaagccaac
     8041 gtattctcga cggtgctgga gctgccaacc agtgagcttg gcgccaatcc taagctcaat
     8101 atctgggggc gatgcagcgt gcggcgagac ggcaagctgc ttcacgtaga tcgcgccggg
     8161 cacccttcag tcagcagctt cttcaacacc gatgacacca aggaggaata caatgccagc
     8221 gagcccgtta acgatcgcaa gcgctggacc gatcagttcg tccacttgat ggggcatacc
     8281 ggcaattact ctcgggaaga ggcaatcgcc gcgatcgacg tcgaagggct gttgcccgac
     8341 atgctgagct tcaatccttc aaagcccgca aaatacccga atggccgcgt cttcaccgac
     8401 gatgtcatcg attaccgcct tgctttcctg tccaagggtg acatcccgcc cgacggcctt
     8461 aagccgcaca ccgacactct caacgagttt ccgtacctcg gtacgccgca tccgaaaaag
     8521 agctaacatg agtctgtcgc gcacgcgcgc gacagacttt tttgggtccc gccaactaca
     8581 tccacccgat ggacggaatt acagttgtga aatccccaac gcgcaggtaa tcgtgctgag
     8641 cgtcgccgtt ccggttaacc tacgagatgg cctgattgcg acagctatct tcggggctag
     8701 aaaagccgga gacagattgt cccgagccta gaacacgaat ggtacaagcg cctttacccg
     8761 agctttgtag gcgggatatt cgtccggaaa gtaactcagg aggaggcttt cttcctgctt
     8821 cagtttgatc cagaagccaa tgaagaagag caagagtccc acgaacgcga ccagcaagcc
     8881 ggacgcaatg gcggttccca gacccatgag cagatggctg gtataaatcg ggtgacgcac
     8941 gaatcgatat ggcccccgtt ccacaagtgt gtgcccctgt ttcaactcaa catctctgct
     9001 ccaatcgcct cctagcgttt tgcgtgacca aatagccgcg aagagtccca cggcacaaat
     9061 cgcaacgccc agatactccg cgatgacggt atgcggtatc accaccatgc caaacggatg
     9121 cacccatgcc gcaacaagca ggatgtagcc cagccaaact ggcaacctgg cgaacctgct
     9181 ggcccacctt tggtgctctt ccgcaggctt gcttgagcgg gcgctaatat tccaatacag
     9241 gatgagcaga acccaacaag caagtatcaa aagtccgggc aacataaatc ggcgcccctc
     9301 tccacccttg cacccttcaa ttctcggccc cgactataga ccttgcaata gctgctctga
     9361 aatccacacc tcgccatcgc caaactacct ggctagcgtt tgcgcctgcg attaccgcat
     9421 gcgccggtag cgattgaact ggtggtcgcc ggattttcgg ctgctaagcc aagtccatct
     9481 cgtcattgtc tccctggtga agccgggacc ggcgaagacg gtgggacgga gaagtggccc
     9541 ttcgcgggct tcgctcggca gacgttcctg ttccccggga acctcttgcc cgatttcaag
     9601 tggtgacacg gtgaggaact tctcatccac cagatcggcg cgggcaaggt tgccgtaaag
     9661 cgtcgggccg ccttcgcata gcaggtactt gattccgagc tgcgctctca attcacggac
     9721 ggcggcggag agatcgacgg attcgccggt tccgggagcg atgatggtga cgtgaggatg
     9781 ggtcttcaac gcgctgaggc gttcagtgcc ggctggcgag gtgacgattg cgacgtccac
     9841 agcgtcgccg tcgaagcatt tgaactgcga gagatccaga tcaagggcgc gggtaacgaa
     9901 gatattgcgc tggcgactct tgccgagctt cttgcgaaac tgcagcaggc ctggatcggc
     9961 gacttggaac acgatgccgc gactctcagg cccgcgatta cgttgctcct cgcgcagggt
    10021 gttcatgccc atgatcacgc cgtcggcgtg agcgcgcagc aggtccatca gccagcggtc
    10081 ggcgcgggac tgcgcaattt ctgcgcccga tgcgtgcttt cccagcaggc tgacgattcc
    10141 gtccagcgat tgcacgaaat tggtgtagat ccagggacgg tcggcaggag gagcgggaaa
    10201 tccaaaattg ccgtagggcg cgagggcgtc gtccgcgaac ggaccgggct cggcgcggtc
    10261 gaagagcacc tcgaagatcg gactttggcc ggagcttccc atgggtacat cgattctatc
    10321 gtggtgtcgt ctgtcgactg gcatgagcat agttgtatga tggaaaactt ctcgcaggta
    10381 ctgattcttg ccgacgcagg ggggagtatg ggtgaacgtg gtggagcgaa tatcgaaatc
    10441 gctcacaagt tgaatgaggc ggaagagcac ggaccgcatc ccaaatcgaa atggattgag
    10501 acactggaga ttgcggaagc catcattctc gcgctcgtag cgattgctac cgcgtggagc
    10561 ggctaccagg cggcgaagtg ggacggccgg caagacgaac tgtacgagca gtccacgaga
    10621 ctccgcgtcc aggcacaggg tttggcgact cgcgcgggac aggaacaaat ctacgatgcg
    10681 tcgactgtgg tggagtggat caaggcgcaa tcgagcggga acaagaaatt ggccgaactg
    10741 tttgaacgcc gcttccgtcc cgaggccaag gtggcatttg aggtttggaa gaaacttgac
    10801 ccgcttaata atccagatgc gctcgcgggg ccgttgctga tgccggaata ccacaatgcc
    10861 catgcggagg agtcagccaa gttgtcgcag gaagccacgg aagcattcga gcaaggctcg
    10921 aaatcccgga gcacctcgga tgactatgtg cgggtcacgg tgctgctggc gacggtgctg
    10981 ttgctgactg ccattgccca gcgattcaag acgcatcagg tgcgcgtggg cctggccgtg
    11041 gttgcattct tactggtttg cattccgatc tataggatcc tcaacttgcc tcgcttgtga
    11101 gcagcaccga ccttgcgggg aaggccggaa cgtgatggac atcaaattaa gtgacatcga
    11161 agcccgcgtg ctgggagcgc tggtggagaa agacatcacg acgccggatt actatcctct
    11221 gtcgctgaat gcgctggtca atgcatgcaa tcagaagaac aaccgcgagc ctgtcatgaa
    11281 tctggaggaa ggcgcggtgc gggatgcgtt gaacgggctg catgacgatg gtctggcggg
    11341 gccggccagt ggcgccgaca gtcgggtgac caagtacgaa caccggttgc aggagacctt
    11401 caacttctca cgtggagaaa ccgcagtgtt gtgcgtattg ctgttgcgcg ggccgcagac
    11461 tccgggcgag ttgcgtggcc ggacggagcg catgcatcac tttgaagacc tggacgcggt
    11521 gcaatcgagt ttgcagaaat tgatgcaacg cgagcctccg ctggcggcgg tgctgccgcg
    11581 gcagccggga acaaaggaat cccgttacgc gcatttgctt tcaggcgagg tggaagcggg
    11641 cgagctgccg gcggcgcgtg cggttccgtc gtcgagtgcg tcgggcggcg acgaacgctt
    11701 gagccgattg gaagaagcgg ttacggcgtt gcggaacgaa gtcggcgaac tgaagcagca
    11761 gttcgcggat ttccgtaagc aatttgagtg aggccccgga tcctcgcgct aatcgtcgcc
    11821 gtgtgccaag gcaacgggcg agagatccag cgaatgggtt tcctcccacg gcggcagcag
    11881 tttcaccagt ccccacaaga tgatgaacgg aagaatggtg agcactatcg ctacgaccag
    11941 tccttcgcgt gtgacgaagg ccatcttgta ggtcgtatag ccgaggaaac ttttggaaaa
    12001 gagctggcct ccgattacca cgttccagcg catggcgaag atgccgatca gggtgagaca
    12061 gccggcgatt ccgtaggtca ccctgcggcg ggctgcgttc agcgggagca cctgggttgc
    12121 cgccaacagc agaatcggcg tcaacgtgcc cagcaatatc tggatgacga tgtgcgggaa
    12181 atacagcttg gtgtgcacca tgaagtcgag gctgcggaag gattcgtcgg actcatagat
    12241 gcggtgaatc aggtcaagca gctcaagtgc aaagtcgatg agaaatgtat agaacagata
    12301 cttggcaatc gtgtccacgc atcgcatgtc gatctgctgc ccgcgtaccc ggcacataaa
    12361 catgtagatc agcatcacga cggcaatacc cgagaccata gccgagaaga tgaacaccac
    12421 cggcatcaat ggcgaagacc accaagggtt ggctttgacg cttccgaaaa taaacccgac
    12481 gtagccgtgc agcaggaagg ctgaaggtat gccaatgatg gtgatgatcc agcccacttt
    12541 attgtcgatg gccagcgccc gttcgcttat atttgtcgag ccgagcgtga gtaccttgta
    12601 gatccagcgc gtccagccgg tggtcgtttg tgaccgcacg acgatgttgc gccggtagtc
    12661 gagccagacc tcgagcacca ggactgccat caggtaccac atgtaaacga agccgaacat
    12721 ggccatcgcc gacgaggtgt gcggcgtcag gtacatctcg agtgagcgct cgggatgtcc
    12781 gaggtgcaat tgcagcggta acggagcgac cagcaggaag gccagcgcgg tcaacagcgc
    12841 gagccggtaa gtcggcttca cggcgtccac gcggaacacg cgctccagcg acgccagaat
    12901 gaaggcgccg gcaaccagtc cagtgaggaa tgggtagagc acgatcagca cgctccactg
    12961 gagctcgatc tcgttggggt acatgaaccc ttcaactggt atgccgtgca agcgtcctcc
    13021 tatcgtaccg agccgtcgag gccgtagtaa tagactttcg ccctggttgc caagtgcggt
    13081 ttcaaaacct gtacgctgtg cgtttcgaga aacttgtgga tcgggtcgtt ggggttcttg
    13141 aagtccacaa gctggcgcgc gcccgtggga caggtctcgc agcaggcagt ggtgagtccc
    13201 ttggtgatgc ggtgatagca gagcgtgcac ttcgaggccg tctcggtctc gggattcagg
    13261 aaacggcagc cataaggaca ggcctggatg cagtagcggc atcccaggca gtacttctgg
    13321 tcgaccagca ccacgccatc cggtgaaaca aaagtcgcgc ctacggggca gacctgggtg
    13381 cagggcgaat cggcgcaatg gttgcagagt ttggggacga agaaattctt gccgccttcc
    13441 tgcttggtgg gagggaagcc gtgcaaggcg ccgtcggggg aatcgacctg cggcatctcg
    13501 gtatcccatt tggcgacgtg gtagcgctcg acccaggtgc gatagtagcc atcggggaca
    13561 ttgttttccg ccttgcaggc ctggacgcag gtgccacaac caatgcactt ggtgacatcg
    13621 atcaacatac cccaccagtg atcggtcatc ttatagttcg gcgattcttc gggcgtgcca
    13681 gcgaggatgg agtcccaggc aatcgcggcg gcaggcagca acatcagtaa gcggcggcgc
    13741 gtgatggtca ttggcttact ccgctgtcga tcttggggct atgcggtttg tggcaggtat
    13801 cgcaaaccat cccgccggaa tggtcggcgg acaccacctg gggaaaggtc ttgggcttgg
    13861 cgatgttggc ttcgtggcag cgaacgcata acaccgcgac gtcgatatgc ggcggctgca
    13921 gcgcggtggg atcgtccgcg tgtttcgcca gcggtccatg acaggactca caggcgacat
    13981 gcgcgtgcac gcctttgctc ttcacttcga gcacatcggt atgacagctt tcgcaagcct
    14041 ggtgcccggc gaacatcaca ggtcgtgcgg cgacttcagc aatggccgcg gcgcggtaat
    14101 gaccgtattg tccaaaggtc ttgggaacaa agatcgcgcg caagaccagg aacaagacga
    14161 tgccgatggc gaagaccgct gccatccgga acaggtgccc tgcatcttta agatttgcca
    14221 taagtttgat cgcggataat tggtgtggct gtcgatccgt acgctggata atgagttacc
    14281 gtgcgaaatg ggcgaaagca catctacttc tttgataaaa gagaacgtcc cgaggcgctg
    14341 tgcgaggcgt cacatcgagc tgtgacgtat cagacatgga aaaacagttg cagagtctcg
    14401 aaagtcaata tcttcgagaa tattttgagc gttcaagcgc ctaaacgctg ttgctgacgg
    14461 cgttctggga taccatacct gcaacaaaat gggcccgaaa gtgatccaat cgggagagga
    14521 tgccgatggc agaaaaaatc aagaagacag aagaagaatg gcgcaaagaa ttgactccgg
    14581 agcagtatcg tgtgctgcgc gagaagggaa cggagcgccc gttcacgggg gactatgaag
    14641 gcgtaaccac ccctggggtg tacaaatgcg ccgcctgcgg gcaggaattg ttcctgtcgg
    14701 actcgaagtt tgactcaggt tgcggctggc cgagcttttt ccagccggcg gccgaagaga
    14761 aggtagatgc ccatgaagac aataccttgg gcatgcgccg tgtcgaggtg acttgctcgc
    14821 actgtggcgg gcacctggga catgtgtttc ccgatggtcc gcgtccgacg ggactgcgtt
    14881 actgcattaa ttccgcttcg ctgaaactgg atccgaaata gcttgagttt tcgcccggac
    14941 gagtccgaga tctacatccc ggggctgaag cccgatcata agagcgacgt agacgccggc
    15001 ctaaaggcag cgcttccacc gcgctggctc agtcgctcgc gtaggaattc gatcaactcg
    15061 ggagtgacat agggtggcgg gtgaatccga aagactggcc tgccctcaac cggcttgagc
    15121 gaaaccttgg tccaatcagg ctgcgttggc ccatcatgca gaatgatggc atcggcgcgg
    15181 tcgaggtact ccttggccga agctttgaag tcgggattgg cggcgtcgag tacggtgaga
    15241 tagaggtcgg gacgcaggaa gcgcatgacg ctgttggact cgaggatggc attttccgcg
    15301 ctggccaatt ccttgcgaag ccgcggcatc gcttccgcca acattccctg gcgagtccga
    15361 acccagattg agcgcgcggc tccggacacc aagaagcggg acgagtcgct ctcaccgctg
    15421 cggtcgcgct cttcggtgac agccaatgag tggtcgtcag tcgcgcagtc gcagggctcg
    15481 ccgttggccg agcagattcc gtggccgtac tgggtgatct tgaaggcggt ccaatgcatc
    15541 tcgggcagcg cggcgataat gccggcgacc acgctggtct tgcccacgct gcgggagtgt
    15601 ccaccgacga cgatgacggc catgacttct attttcctgc cttgcgggtc agacgcgaca
    15661 gcgttgccaa cgcttttagg tattcgcgaa ccggcttgac gggtgtgccc cagtaaacga
    15721 tgcccttgcc gcggatcgtc ttattgggga ggacgccgct ttggcctccg aggatggcgc
    15781 cggactcgat ggtgacgtgg tcgccaagtc ccacttgtcc gcccagcaag cagtcatctc
    15841 ccacgcttga actgccggaa attcccgtct gggcggcgat cacgacgttg gcgccgatgc
    15901 tgcagttgtg tccaacgtgg accatgttgt cgagcttcgt accgggcccg atgacggttt
    15961 catcgagagc tccgcgatcg atggtacagt tggcgccaat ctcaacatag tcgccgatga
    16021 cgaggcgtcc gacctgcggg aacttcacgt agcgcccata ggtttcgtcg cgaacaaatc
    16081 cgaagccgtc ggagcccagc accgcaccgg catgtacgac aacgcggcgg ccgatggtgg
    16141 ttccgggata gaccacgact cggggataga cttcgcaatc gtcgccgatc accactcctg
    16201 ggccgatata gcatcccgag gcaatgaagc agcgttcgcc gacgaccgcg tcggcctcga
    16261 tcacagcata agcatcgatg cttgagctgg gagacacctt cgccgattga tcaatgatcg
    16321 cggtgggatg aattgcgggg gtgaattttg cccgggggta cagcatcgtg gctgcggtgg
    16381 cgaaggcgag gcgtggacgc gaactgatga gcagcggctt tcgtccagtg gaaccggcgg
    16441 cgaattcgcc tgcaatgatg gcgctggcgt tggagttcaa ggcttcgtcg agatgacggt
    16501 cggactcgac gaaaacgaga tcgccttgat ttgcgcgggc aatgctcgct accctttcga
    16561 tccggatgcc gccatcgccg atcaggcggg cagcgatgaa ctcggcaaga gattgcagag
    16621 tctgtgacat gggtcagtta taggcgagtg gctggtgaaa ttcaaaactg tttaccacgg
    16681 aggcacagag ggcacggaga tcggagagtc gaaaggcgat agtaataagt aataagtaac
    16741 aagtaataag cagtaagcaa gggcaatcgc ggattcgcgc ggatgaattc ctttcaatgt
    16801 ttcttggcgt ctaagtgccg ctggggcgaa tgaatctcag tgctaataca gtggcggctc
    16861 gccgggggga cgggtcttcc agcggcggtg tacccaaagc cactgatcgg ggtatttgcg
    16921 cacgtagtcc tcaaaaaccc gattgaaggc ggcggtgttg gtgacggcat cggcctcgtt
    16981 gtcgccggtg cgcaccaatt cgatggcccg atcgaactcg acgcgatact tgcgcagaac
    17041 tgggtcccag atggtgaacg cagggactac ggctgcgtcg gtgtgcaacg ctacgcgagc
    17101 gatgccgacc gcggtgcaag caggaatgcc gaagaaattg gcgaacacac cctgaggcaa
    17161 catcatgttg gtgtccatca agatgccgac tgtctcattg ttctccattg cagctaacaa
    17221 accgcgggcg aatccctgct tgccgatcac agtgttgcca tgcagcgtcc ggtagcgggt
    17281 gaccaggtcg tccacatagg ggttgtcgat ggggcgcacc acgatgcgca tgggatggcc
    17341 ttgcagagaa tgaaaaaacg atccaatctc ccatccgcca aaatgcccgg tgaggaagag
    17401 cacgcccttg ccgcgctttt cggcggcgtc gaaattctcg aagccctggt aaacggcgac
    17461 tttcgagacg ttgtccggtg tgtagcgggg gaagaggcag aactcagcga gcaatcgtcc
    17521 cagcgaccga tagaccccac ggagaatcct gcgtcgctcg ggcacgggtt tgtggggaaa
    17581 ggccagttcc agattgcgca ttcccacgcg ccgcaatcgc ggatgcagat gatagacgat
    17641 tccaccgatg acgagtccca ctccgcgcgc caccgggcgg ggcaacactc cgatgaatcg
    17701 caccagaagg aacaacggcg cgtattgcag tctatagagc atcagggggc gtattgacgg
    17761 tagcaccgga aagcacgggc ggcaaaatgc agcttacgaa gacgacggtt cggctgcaac
    17821 aaaaagcggt ggccgcagcc accgcggtga aaccatccta ctgctagcgc aggttatttc
    17881 cgctgcccgc cgggtcggtc gtagttgccg gtgcgggggt tgcggcgggc gcttcggctg
    17941 gggtcttagc tggctcggta gccggggtcg ctgaggccgt aggctccgca gtcgcagccg
    18001 gggccggagt gggtgcggct gcggaggccg gcgcaaccgc aggcgttgct gcacgccgcg
    18061 tcggagcata gtgctcgatc gagttcagaa aggcttgcga gtccggcggc ggcggcactt
    18121 ctccgttgag cagggaatag aacgtccagt ctttgccata gaaggcgcgg acgtcgccat
    18181 cgtcctggcg aatgactgct ccgctgagcg aaacgccgag aaaaaggccg cgcgaacgcg
    18241 agtaggtgag gacctgggcg cgcatggtga gatcggttgc tgcctgggca tcgcggccca
    18301 ccggcccggc tgcggctgac gcgtcagctc ccagcttgaa cttgctctgg agcaggtgct
    18361 gcatgccgtc atcggtgcga atgatcagca ccaagtcgct ggcttgtcca cctgcctgga
    18421 agccgaagct gccgccttcg atgcggaaga aagcggggct gctccaaccg gtggcggtac
    18481 ggcacgtggc tacacctttg ccgtaattgg cgcccaagat aaagctgatc ttcatcatgg
    18541 acggcacgat ggcaatgcat ttcgcgcccg acatgatgct gtcaggaatg ccgcgatcgg
    18601 gggcctccat aatctggctg aggatggtgg tggcactctg tacgcgttcg acatcagcgc
    18661 tgcgcgcagc cgactgggcc cacatggctg tcgagacaag aaccagcagg gccacggaaa
    18721 ttttcttcat aactcgactc caatctgaat cacggtgcat ccggccagtc gacaccggct
    18781 gtcgccatct tatgtcgttt tgccgcggat tttgccgaat tgaagcgcgg aggctggtta
    18841 ctctctagtg tcgtcacggg agcggttggg agtggcggtg gggtagccgt gggcggtttg
    18901 ggatggatgt gggggcgggc aaaaatttag ggttgaagga ggagaccctc gagtccccct
    18961 ccaaccctat ctcccaggtt ctgtggtagg tgaatgcagt atgcgtccga agcaaaagcg
    19021 tggcaagtta cgaaagtcca aggtgatgta ctttagtaac taaccagcaa aatggcttat
    19081 agtcgcgaga ctgttggaca acgaaaaact actcggattg gcccactccc agcagggaat
    19141 gagtgagggc atgtaccgaa agggcgatcc ggttggtgac accggctttc cgcatcaact
    19201 tcgcgacgtg ggccttgacg gtgcgctcct cgatgcccag ttcgtggcca atctcgcgat
    19261 tggatctgcc gtccagcaac aagtgcagta cctcacgttc acgctcggtg aaaaccaggg
    19321 gctcttgtcg agccgtgctg cggagaacgc tagtgacccg ttcgatgaac atgccgaaaa
    19381 gacgccggga tatccagacc gatccactat gaacttctcg cactgccttc ttgaattcgg
    19441 caaccggggc agattcctcg acatagccct tggcgccggc gcataaggcg cgcaaggcca
    19501 tttcgtcccc gggaacattg cccgtggcaa tgatgcgcac cgaaggatcg agtgccttca
    19561 gggctgccat gacttcgtac attgctggtc cggtgcgcga tccgatcagg acgagatcga
    19621 aattacggtt accgaagatc gaagtggaag tgcaactctg gacctcgaaa tcagggtccg
    19681 catcgaaaag cgcacgaaaa ccgacagaac gaaggggatc gttttccacc agcgcgatcc
    19741 gaatcgggtt cttcacggcc gatttcatgg gagggggccc tcggtgctga catggataga
    19801 gtagtacaaa accgccagaa ttcatgtggg ccttctgtgc catgccacca ggcatctacg
    19861 gcgcagcaac gagcctgcac tgagtttgtt aggatacgct gcataacaac gagctaactc
    19921 gcttcaagat tggggtttaa atcttgttac agagtcatct tttgggaaca cgggaccacg
    19981 cttgctaagt ttcgatcagt ccagcgcctt gccggcggcg gtggtggcga tagggtttgc
    20041 catgctcgcc cgtttcatgg gagcggtgag tgatggtggc gcactggtgg ggatcgtcat
    20101 cgcgtttctg gtgatggttg cggccggcat gggcgggttg ctcccgctgc tggcagtgct
    20161 ggtgatgacg ctgctggcca cacgttggcg taacgagcgc aagcgcagca tgggagtgtc
    20221 cgaacgcggc agcgggcgaa ctgcgtcgca ggtggcggca aacctcggcg cgacggcatt
    20281 gtgcgcggcg gcagtcattg tttttccgcg gcattccgga atctttatgg tgggagctac
    20341 ggctgcgctg gccgaagcgg ccgcagacac cgtgtcgagc gaggctggcc aggcggctgc
    20401 gaagcaggcc cgcatgattg tgaattttca gcctgcgccg atcgggacca atggagcagt
    20461 gagtcttgag ggtacattgg cgggatgcat ggccgcattc atcgtggcgt gggtgagttc
    20521 gttcttcggt gtcgtggctg gccaatgggc tccggtggtt gcggtcgcgg gcatcgcggg
    20581 catgttcttc gacagcgtgc tgggagcgac gctggagaat cgcgggaaaa tgggaaacga
    20641 ttccgtcaac tttgtgagca cagtatttgc ggccgatctg gcgatccttg tcatcctgtt
    20701 tgtcgagggc gtgagcggct aggttgaagc gcccgcagtt ttgctgttcg cgtcattgct
    20761 ggataaatcc gccgtttcgg agtggaacgt ggcacgaatc aaactgatgc tgtttctgtt
    20821 gtgcgcggtg gctgccgcgc cgctgttggc ccagcagtat gacgtgctca tccgcaacgg
    20881 acgggttatc gatggcagcg gaaacccgtg gatttatgcc gacgttggca ttatcgggga
    20941 tcgcgtcgcc ttccttggac atgctggcac aggcgtgacc gccaagcgca ctatcgatgc
    21001 caccggactg atcgttgcgc cgggtttcat cgacatgctg gggcagtcgg agtttaacct
    21061 gctggtggat aagcaggcgg tcagcaagtt gacccagggc gtaaccacgg agatcacggg
    21121 cgagggggag tcgatcgctc cgaccaatga cgcgctcaac gcggaacatg ctgatgttct
    21181 cgatcactac cacgtgaccg gcgactggcg ttcgctggaa gaatactttc agcggctgac
    21241 caagcagggg agcgggatca accttgggac ctacgtcggt gctgcgcaaa tccgccgcct
    21301 ggtgattggc acgagcgatc gcccgcccac acccgaagaa ttgaaggaca tggaaatcgc
    21361 cgtggacgac gccatgagcg acggcgcgat gggtgtgtcg agcgcgttga tctatgctcc
    21421 gggctcgtac gccaagaccg aagagttgat cgcgctggcc aaggcggcag cagccaaggg
    21481 cggcgtttac gcttcgcaca ttcgcaacga gggcgacagt gaaaccgagg ccctgaatga
    21541 agccttccgc attggacgcg aggccaacat tccggtgcag atctttcatc tgaaaatatc
    21601 gggcaagcaa aactggggca agatgccgca ggtgatcgac gaaatcgaac aggcgcgggt
    21661 cagtggcgtc gatgtcacgg cgaaccagta tccctatatc gcgtcggcga cttcgctggg
    21721 agcgatcatt ccaccgaaat ttcatgacgg cggtacagac gcgttcgtcg cgcggttgaa
    21781 agacccggcc acgcgggcac taatccgcac ggatctggaa ggtggcagcg gcgcgttcga
    21841 aaacatgtgg cgcggcaccg gaggcccgac tggcgtgatg gtggtgtcgg tgttgaatcc
    21901 ggagtaccgc aagttcgagg gcaagaccat cgcgcagatc gcgcaaatgg agaacaagga
    21961 cccgctggat gcggcgctcg atttgattgt ggccgatcgc gacaatgtcg gggccgtcta
    22021 cttcagtatg agcgaagaag acgtgaagct ggctatgcgt caaccatggg tgagtgtggg
    22081 cacagactat ggcgaagtga gcccgaccgg gccgctgggc gagtcgaagt cccatccgcg
    22141 ggcgtatggc agttttgcgc gcattctggg caagtacgtg agggaagagc acaacctgcg
    22201 gatggaggac gcgatccgca agttcacgtc gcttccggcg caacaagtga agctggaaaa
    22261 ccggggactg ctgcggtcag gctatttcgc cgacgtaacc gtcttcaatc cagagacagt
    22321 caaggacatg gctacgttcg aggacccgaa ccgtacgtca gtgggattcg aatacgtctt
    22381 cgtcaacgga gtactgtcgc tggagcacga taaggtcacg ggccagatgg gaggacgtcc
    22441 gctgcgcggt ccgggctata tgatgcgcga ctacgttccc gatggactgc cggtgcgcgg
    22501 caaagtgcag ggagtggtca ccgacgaggg gggctatccg ctgccgcgat cgaccgttac
    22561 ccttaccgat gcgtcgggca aagtgctggg cacctcgaac atcaagaagg atggccgcta
    22621 tgaaattgtg ctggaacagc cgtgtcatgg atgctcgttg aaatcggagc gggcgggatt
    22681 tgtaatgcag accaagaccg ggatcgagta caacgggtcg aattcgcttt ggtttgggtt
    22741 tgcgttgaag agaagcaatt agcgactagc aattagcagt cagcaataag ccgaaacggc
    22801 ccgcatcttc gaacgatttc cggtgtttgc ttcttaaggc gcgtccgata tgtcaaagcg
    22861 gattctcgga aacaaatcat ggccgccggg cgtccaatat tgcgtgggat atatttgccc
    22921 ggcgacatag gtggcgtctg ggctacagac gaatgatcaa gtggaagaca tttacgatag
    22981 gctggagcct ggcgctggcg ctgttgctgt tcgcggcgca caagacgact atgcctgacc
    23041 agagctccta tcgcgacgtg gtcgatctga cccacaacct gaacgaccat tcgcccaact
    23101 gggaaggcag cgagcagtcg cccttccatg ccaaacaact gggcgacatc gagcacgacg
    23161 gctactactc acgcatcttc accacgcagg agcactacgg cacgcatctc gacgcgccgg
    23221 cgcacttcgc gcgcggcatg tggacggtgg accagattcc cgcggagcga ctggttcgtc
    23281 cgctggtggt gctgaatgtt cgcggcaagg cgaacgacaa tcctgactat gaaatcagcg
    23341 tgcaggatat cgctgattgg gagacccaga acggcgagat tcctgccggc gcggtcgtca
    23401 tggcttatac cggatgggaa gaccgctgga gtgctcccaa agcgtttcgt aacgccggcg
    23461 ccgatggcgt tccccacttt cccggatatt cgctcgaagc cgtgacattc ctggtcaaga
    23521 cacgcaacac ggtgggactc ggcattgaca cgatgagcat ggatccgggc gcaagcaaga
    23581 cgtacccggt tcaccagttc acagccaaag agagcgttta ccagttggag aacgtggcca
    23641 acctcggatt ggttccagct gcgggcgcta cgattgttgt ggccccaatc aaactggaag
    23701 acggttcggg aggcccggcg aggctactgg cgctggtgaa gtagcgagtg attggaaatc
    23761 ctgaaatgtc gccacagccc aaaatttcca atcgccggca attctgaccc acaaacgcgg
    23821 ccacttacgc gggctgaaag cctgccagtc gcaatgcctt ctggtcgaac gtcagagtgt
    23881 gctgacagcc ggccttcgcg cccagcgcgg cgatcagcgc gtcggcgaaa tcaccccgtc
    23941 ccaggtggag ggcgatcatc gcactgaaga cttcctgctc gttctcgatc accagcacct
    24001 cggtctgcag gaggagctca ac
//
LOCUS       DASRKK010000695         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_949174, whole genome shotgun sequence.
ACCESSION   DASRKK010000695 DASRKK010000000
VERSION     DASRKK010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949174"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(516..1295)
                     /gene="istB"
                     /locus_tag="VFC15_16875"
     CDS             complement(516..1295)
                     /gene="istB"
                     /locus_tag="VFC15_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM11884.1"
                     /translation="MKAVVENLEKAAVEQQCRALRLPTIAEQCAPLAETAVKQRQTYL
                     SFLEALLGAELEERERNTIARRIKEAHLPRIKTLEEFDFSQTPQIQATRIHELANGGY
                     IERAEPLIFIGDCGTGKTHLLTGLCIAACRQKRRVRFATAAALVNELVEAKQQLQLRR
                     VLARWERYDVIAIDEVGYVPMADLGAEFLFQVIAERAEKAAVILTTNLPFSEWTQVIP
                     NARLCKALLDRITDKAHIIETGTESYRFRRTVEKGRNKKEG"
     gene            complement(1292..2848)
                     /gene="istA"
                     /locus_tag="VFC15_16880"
     CDS             complement(1292..2848)
                     /gene="istA"
                     /locus_tag="VFC15_16880"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM11885.1"
                     /translation="MEQRKRSWLTRRDKVELFEQIRREYEFGVGTISGISRKLGVHRR
                     MVREALNSAVPAESKPQRRHLRKLEGATAFIDRILNEDRQAPPKQRHTARRIWQRLCM
                     ELPGFQVSERSVSGYVRRRRQHLGLISREVFVPQSYAWGVEAQCDWYEAWAVLGGERI
                     KLQVFEMRSMASGGAYHRAYTHATQQAFLEAHERAFGYFDGVFRLLRYDNLSSAVKRI
                     LRGHQREETSRFIAFRSHWRFQSEFCNPARGNEKGGVESEGGYFRRNHWVPLPEAGDL
                     EELNTYLENCCQQDQRRILDGRTETVGTAMLHEQPHLLPLEPEPFDLAEISFPTVDGL
                     RCVRVRTNRYSVPLPPGTVVEARVRADYVELWQKGVRVACHERCYRRQQKILDLEHYL
                     DVLRKKPGALAGSTPLAQWRQAGRWPECFDHLWQALNVRHGRQEGTREMIELLSLGTG
                     EGWDRLRAAVEQALSLGCQDVAAIRHLLAASRLVKPCVAAIEIGVLACYERPQPVLSD
                     YDQLLATVRA"
BASE COUNT          700 a         1093 c          930 g          722 t
ORIGIN      
        1 actttcaggc cgcatgccat ggtttctccg ccggggaaag agggctgctc ccaggtccaa
       61 caccctgctt atcgacggat tggttcgcag cgtcacaacc gagttacaaa gtggcaaacg
      121 ggaatcccct cctgcaaacg agcttcgaaa tctggtgagg ctcttcctga gatacataag
      181 acgcggccga atcaatctct ggattcggga ctgcgcgcac aatcctgccg gagtcgcacc
      241 tcaattagca gactttgtca gcagacggct tggaaacttc ccactcaaac caacaattag
      301 aaaggagcca taaatatggg taaatcgcat agcgtttctc atcgcaaaca acatcacgca
      361 gccctttgtg tatggcaact taatttggcc cacttgtgga agttaactct ggcccatcta
      421 tggaagctta ctgggccgga ttctatggtt gtggagatgc gatgtgtctc gggctggagc
      481 gtagcggaag cccgggatac atcgagtgtg gtggatcaac cctccttttt gttgcggcct
      541 ttctcgacgg ttcgccggaa gcggtagctt tcggtaccgg tctcgatgat gtgagcctta
      601 tccgtgatgc gatccagcag agccttgcat aggcgtgcgt tggggattac ctgggtccat
      661 tccgagaagg gtaagttggt ggtcaggatg accgccgctt tctcggctct ttcggctatg
      721 acctggaaga ggaactccgc acccaggtcg gccatcggca catagccaac ttcgtcgatg
      781 gctatcacgt catagcgctc ccagcgtgcc aggacgcggc gcagttgcaa ctgttgcttg
      841 gcctcgacca gttcgttgac cagggcagcg gcggttgcga aacgcacccg ccgtttctga
      901 cggcaggctg caatgcacag cccggtgagg aggtgggtct tgcccgtgcc gcagtcgccg
      961 ataaaaatca gtggttcggc acgttcgata taacctccgt tcgccaactc gtggatccgc
     1021 gtggcctgga tctgcggcgt ctgggagaag tcgaactcct ccagcgtctt gatccgtggc
     1081 agatgcgcct ccttgatgcg ccgggcgatg gtattgcgct cgcgttcttc cagctcagca
     1141 cccaacagtg cttccaggaa actcaggtag gtctggcgct gcttgacagc cgtctccgcc
     1201 aagggagcgc actgctcggc gatggtcggc aaacgcagtg cccggcactg ttgctccacg
     1261 gcagcctttt ccaggttctc cacgacggcc ttcatgctcg caccgtcgcc agcagctggt
     1321 catagtcact cagtaccggt tgtggtcgct cgtagcaggc cagcacgccg atctcgatcg
     1381 cggcgacgca aggctttacc agccgactgg cagccagtag atggcggatg gccgccacat
     1441 cctgacaacc tagcgaaaga gcctgttcca cggcagcccg aagtcgatcc cagccttcgc
     1501 ccgtgcccag agaaagcagt tcgatcatct ctcgcgtgcc ttcttgccga ccatgacgca
     1561 cgttcagtgc ttgccacaga tgatcaaagc actccggcca acgtcccgcc tggcgccact
     1621 gtgccagcgg ggttgaaccc gccaacgcgc ctggcttctt ccgcagtacg tcgagataat
     1681 gttccagatc gaggatcttc tgctggcggc ggtagcagcg ttcatgacaa gccacccgca
     1741 cccctttctg ccacaactca acgtaatcag cacgcacccg agcttccact accgtgcccg
     1801 gcggcaacgg aacggagtag cggttggtgc gcacccgtac gcagcgcagt ccatcgaccg
     1861 ttggaaaact gatctcggcc agatcgaagg gctcgggctc aaggggtagc agatgcggct
     1921 gttcgtgcag catcgccgtg cctaccgtct ccgtgcgacc gtcaagaatg cgccgctgat
     1981 cctgctggca gcagttctcc agataggtgt tcaactcttc caggtcgcct gcttccggca
     2041 ggggaaccca gtggttgcgc cggaagtatc cgccttcgct ctccactccg cccttctcgt
     2101 tgccacgggc ggggttgcag aactcgctct ggaaacgcca gtgcgatcga aacgcaatga
     2161 agcggctcgt ctcctcccgc tgatgcccac gcaggatccg cttcaccgca ctcgacaggt
     2221 tgtcgtagcg caacaaccgg aagaccccgt caaaatagcc aaacgcccgc tcgtgcgctt
     2281 ccagaaatgc ctgttgtgtg gcatgggtat aggctcggtg ataggctccc ccgctggcca
     2341 tcgagcgcat ctcgaatacc tgcagcttga tccgctcccc acccaaaacc gcccaggcct
     2401 cataccagtc gcactgcgct tccacgcccc aggcatagct ctgcggtaca aatacctccc
     2461 gcgagatcag tcctaaatgc tgacgccgtc gcctcacgta gccgctaacc gagcgttcac
     2521 tcacctggaa gcccggcaac tccatgcaca gtcgctgcca tatacggcgt gccgtgtgac
     2581 gctgcttcgg cggcgcttgg cgatcctcgt tcagaatccg gtcgatgaag gccgtagccc
     2641 cttccagctt ccgaagatgc cgacgttgcg gcttgctctc cgcaggcact gcactgttca
     2701 gtgcctcgcg aaccatgcgc cggtgaactc ccaacttccg cgatatcccg gaaatcgttc
     2761 ccaccccaaa ctcatactcc cgtcgtatct gctcaaacaa ctccaccttg tccctcctgg
     2821 tcagccagct ccgcttccgt tgctccatcc acgaagccta tccagctact accaagtggg
     2881 ccaaacttaa cttccacagc cgggccaaac caaactacca aactcatccc gactctcatc
     2941 cgtgttgggc gtgttctcga tgtcttagca atcgctccag agtggcgggc aatgcgggat
     3001 ttgactgtca cttgatgaaa acaggacttt gcatgaatta gctgcttctc gtcaagaaaa
     3061 agaagatctc cggaaaagcc gatcaatgag gcggatctgt gtctgccaac acgaagttgg
     3121 ccagaaaccg gcatgtggga gattactccc gggaagtcgg acagcatccg gcaaccgcat
     3181 tggcaccgcc acttggacgt aaaacgccca ttccgctgaa gtggacggcc aaactcccag
     3241 aaatctgctt ggaggaaact ttaccgcgcc tccgcgaatc ccgttagcga atctcgacat
     3301 tcccgaaccc aaggcccatc agcatgctgg taagcgtctg gcgtgcattt ttgtcggcga
     3361 tcttcaggat gccgtcctgc aatgcggctg cctgaagttg gcgctcagct tcctggcgta
     3421 cctcgccttc aagattcgga tcggc
//
LOCUS       DASRKK010000696         7691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_949183, whole genome shotgun sequence.
ACCESSION   DASRKK010000696 DASRKK010000000
VERSION     DASRKK010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949183"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..375
                     /locus_tag="VFC15_16885"
     CDS             157..375
                     /locus_tag="VFC15_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11886.1"
                     /translation="MLTYNVLTALKRLALPAELIAARPKRLRFLIFCTPGKLVHHARR
                     TLLRLARSWQRFSNWHYALRLLPLPAYG"
     gene            1234..1818
                     /locus_tag="VFC15_16890"
     CDS             1234..1818
                     /locus_tag="VFC15_16890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM11887.1"
                     /translation="MQFFAFDDSYVERLRAGDFRTQEHFVSYFSELMRLKLRSRLNSA
                     QAIEDVRQETFARVFAALRSGGGIRDAERLGAFVNSTCNNVLLEAYRSSSRNPAAPDE
                     GSHDPPDEAVDALTGLIQTQTAEKVRQVIDELPERDRRLLKEVFFEDRDKDQVCHDFG
                     VDRNYLRVLLHRAKQNFRDLYLKTAVKVGQASGE"
     gene            1872..2558
                     /locus_tag="VFC15_16895"
     CDS             1872..2558
                     /locus_tag="VFC15_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM11888.1"
                     /translation="MNHSEALQLMATEKYLLDEFPPEIRDEFEEHFFGCPECALDVHM
                     AATFLQHGKKALSAAEAKPIVAAPVKSGWAAWLRPAFAIPVFALLLAVIGYQAFVVRP
                     TLSNTIAELEQPQILASAYLSAGTARGDSQPVVIAQAGQPYILFVDIPARNTFSAYSA
                     ELLNPAGTKLWSLKVPSETVESAKDSLPIRVAPASNEAGDYILVVRGIAAGETEGPEI
                     ARYLFKVQLR"
     gene            2588..3439
                     /locus_tag="VFC15_16900"
     CDS             2588..3439
                     /locus_tag="VFC15_16900"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HZM11889.1"
                     /translation="MDNQVRRTHFYHADASAVGGRIDRPFQQILPVQAPLSLPAVGGF
                     ATARVGDFRFDGILSFQAAQTQVSGSFDERNGNWTTLVSASIEGLNILNVITADRVVA
                     QISTEHPRDGYTPRVSFVGTQFENLRIGGCPINPVLDLGLCDQAGAGDYPKQPCIENP
                     AFLKRVGEFHQNMRSGQTDANDPAWHRCLDWLEERYPLPQADESRLKERSAVLCSLVQ
                     EIQGDCPGKRFGHALVIPEFGKVFLGELLLDHNSFHLIMLRLEMGSPTKAVITSGDTR
                     TNGSTYP"
     gene            3532..5952
                     /locus_tag="VFC15_16905"
     CDS             3532..5952
                     /locus_tag="VFC15_16905"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZM11890.1"
                     /translation="MTIVQGDLIKAQQEAKEGYARYSQQSAQWAWKFRILEAEILTYR
                     RLNQEALSTLNSRLPADLASGDEAIQKNMVEGLALARLGRQDEANERLDKADQLCQLS
                     HSHVDGQLAQIHGVIEFGRGDVAQSEAYFRQVLRMAREEKDQLLEATALMNLGLAALK
                     QGHYDNAILWSTDGYEAARNLGARFAEQKALGNLGWAYYRMGDREKSLVYFQQAAEEA
                     RKLDAKNDLAKWIRALGMISQEMNQTSTAETYYKQSLSLAQQSDDKEDLVDVMTALAA
                     VSVEQQKWEQANRYTQQAMSLCRSRGDREGELEASLIMGKIALHQNDAARAVQLFSEV
                     AADPQNEAPLKWEAQEDLARLYEDEKRPADASRQYKLSLATFESTRASVNQEELRLPF
                     LANSAHLHDDYIHFLVNQGSELEALQVADYSRAQTLAEGLKSGNSKDSAKPTGIGGPQ
                     KLASQVNATILFYWLGPRYSYLWIVTPDHVKLSPLPSAEVLNALVGNYRKDIQGPRDA
                     LQAANQNGFELYKLLVAPASQEIANTSLTIVISDGALNDLNFETLLVPGPNPHYLIDD
                     ATIVNATSLRLLKTSAQAPIARPKLLLIGDPVSPNRDYAELADAGAEVRSIEGHFAPT
                     ERTVLTRGEATSRAYFGSNPQQFSYIHFVAHGTASRLSPLDSAVVLSKSAAEDDSFKL
                     YARDIIRRPIQADLVTISTCYGAGARAYTGEGLVGLSWAFLRAGAHNVIAALWQASDS
                     ATPQLMDGFYKELEKGHSPDVALRSAKLSLLHSDSVFRKPLYWAPFQIYSDQIKPRAS
                     GKTPQHHR"
     gene            7076..>7691
                     /locus_tag="VFC15_16910"
     CDS             7076..>7691
                     /locus_tag="VFC15_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11891.1"
                     /translation="MTPQISKQHSNVVAAQPPTTLPKEDRMKAKRHLMFFALMFALVA
                     LNGRLNAQFPVKEFVPFNSFVEETKAANTSDYTARSTSKVRDAAAFEEMRQHILNLYQ
                     GVQVSHSFVVESQTYDCIAIEEQPSVRLLGLKGIAAPPPASAGPAERVGAPDNSRAQS
                     GQTAVSSIFPAQGVDRFGNTIGCQANTGPVGRTTLEQMTRFPTLK"
BASE COUNT         1686 a         2191 c         2231 g         1583 t
ORIGIN      
        1 caatcggtgg gactggacgc cgaagaagct gctgcaatgg catcgcgaga aggcgggttc
       61 gattgaggcg gtgcatgacg tgatcaagaa cgagttggcg ggcggggtga tgccgtgcgg
      121 gcgcttcgga gccaacgcgg cctggctgcg actgcggtgc tgacttacaa cgtgctgacg
      181 gcgctgaagc ggctggcgct gccggccgaa ctgatcgccg cacgaccgaa gcgtttgcgg
      241 tttctgatct tctgcacccc gggcaagctg gtgcatcatg cgcggcgaac cctgctgcgg
      301 ctggcgcgga gttggcagcg cttttccaac tggcactatg ccctgcgctt gctgcccttg
      361 ccggcgtacg gctgacgcaa gcgaaatcca gccaagcgaa gccaacccag ccgcccggag
      421 cgagggcggg aagcagcctg tccgaaaata cactgcccga ggactggcgc cagcctgtaa
      481 gtcggcttgc ggatgccatg agctgtaccg cgtctcacgc cgcccatgct tgatcgctgc
      541 acgaccaccc gaagtcgtct tcaacttctt acggacggat tacggcaggg caagcagaac
      601 cggctcctgt tccccttgcc gaatcgaggg gcaagtgggg accacggaat tgtgcgttgc
      661 aaggaacgac tgggtggtct gctgaagtac tatgagcggg aagcggcatg agccttcggt
      721 cgggtaaact tcccttcctt gcagccaaga tgagtttttt taccatacgg ggtcacaaaa
      781 gcctccttgt gacccaagcc cttacacagt ctataggctg agggacgatc gacgccgcag
      841 catccgccgc gccggaggct tagttagttc aaacgcgcta gcatccatcg actccgagtt
      901 tcctactcca tgcgttgtcc acaatcgtgt cagttgatga aaaacgccga ttcacaagtg
      961 atcgtggttc gtctgccgtg acggcgtgag tgtaggccat cgtggtttgc gccccgtctg
     1021 tgagggaggc cacgccacgc ggcgggcgat tgcgaagaaa tcgcgtgctt tttcggttcg
     1081 tttgggcacg gtttaatgat gccacggatt cgtcagcggc gtaaccccct gtggaaatca
     1141 aactgaccca ctacccgcga tccgaagttg ctacatcctg tccaatcacg gtgtaagatt
     1201 gctagcatct tcaaatcccc tgagggccag cgcttgcagt tcttcgcctt tgacgactcg
     1261 tacgtggaac gactccgtgc gggcgatttc cgcactcagg agcacttcgt cagctatttc
     1321 agcgagttaa tgcgcctgaa gctgcggtct cgcctgaatt cggcgcaggc catcgaggat
     1381 gtgaggcagg aaaccttcgc tcgagtattt gctgccctgc gatcgggagg cgggattcgg
     1441 gatgcggagc ggctgggggc gtttgtaaac tcgacctgta acaacgtgct gctggaggcg
     1501 taccggtcgt cgtcgcgtaa tcccgccgcg ccggatgaag gcagtcacga tccgccggac
     1561 gaggctgtcg acgccctgac cggactaatc cagacacaaa ctgcggaaaa agtccgccag
     1621 gtgattgacg agttgccgga gagggaccgc cggctgctga aggaggtgtt cttcgaagac
     1681 cgtgataaag accaggtctg ccacgacttc ggagtggatc gaaactatct tcgggttctg
     1741 ctgcaccgcg ccaaacaaaa ttttcgtgat ctttatctca agactgcagt caaggtgggc
     1801 caagccagcg gtgaatagaa aatttgtcac ggtttatggc atttccgcac tattagaagg
     1861 aggctggaaa aatgaaccac agtgaagcgc tccaactgat ggcgacggag aagtacctcc
     1921 ttgacgaatt tccgcctgag ataagagacg agtttgaaga gcactttttt ggatgtccgg
     1981 aatgcgcgct cgatgtgcat atggccgcga ctttcctgca acacggcaag aaagcgctgt
     2041 ccgcagcgga agccaagcca attgtcgccg ctccggtgaa gtccggctgg gccgcctggt
     2101 tgcggcccgc gtttgccatt cctgttttcg cacttctttt agcggtcatt ggataccagg
     2161 cattcgttgt ccggccgacg ctgtcgaaca ccattgccga gctcgaacag ccgcagattc
     2221 tggcgtccgc ctacctgagc gctggaaccg cacgcggcga cagccagccg gtggtaatag
     2281 cccaggcggg tcagccctat atcctgtttg tggacatccc ggcccggaac accttcagtg
     2341 cttattccgc ggagttgttg aatccggcgg gcacgaagtt gtggtcgctc aaggttccat
     2401 ctgaaacggt tgaatccgcg aaagactcct tgccgatccg cgttgcacct gccagcaacg
     2461 aggctggaga ttacatcctg gtagtgcgcg ggatagcggc cggagagact gagggccctg
     2521 aaatcgctcg ctatctattt aaggtacagc tgcgctaatg gcgccctgaa cgagaggaac
     2581 gtgcactatg gataaccagg ttcgcagaac gcacttctat cacgccgatg caagcgcggt
     2641 tggcggccgg attgatcgtc ctttccagca gatcctgccg gtccaagctc ctctgtcgtt
     2701 gccagcagtg ggaggattcg cgacggcgcg ggtgggagat tttcgatttg acggcatact
     2761 gtcctttcaa gcggcgcaaa cgcaggtgtc cggcagcttc gacgagagga acggcaattg
     2821 gacaaccttg gtgtcggcct cgattgaggg gctgaacata ctgaacgtga tcaccgccga
     2881 tcgagtggtg gcgcaaatct caaccgaaca tccgcgtgac ggctacacgc cgagggtttc
     2941 cttcgtagga acgcaatttg agaacctgcg tatcggaggc tgcccgatta atccggtttt
     3001 ggatctcggc ttgtgcgatc aggctggtgc aggcgattat cccaagcagc cgtgcatcga
     3061 gaaccctgcc ttcctgaagc gggttggcga atttcaccag aacatgaggt ccggtcagac
     3121 agatgcgaat gatccggcat ggcatcggtg tctggattgg ctggaagagc ggtatccatt
     3181 accgcaggcg gacgagagcc gacttaaaga aaggagcgcg gttctatgct cgctggtgca
     3241 agagatccag ggagattgcc ccgggaaacg gttcggccat gccctggtca tacccgagtt
     3301 cggcaaagtc ttcctgggcg aactgcttct cgatcacaac tccttccacc tgatcatgct
     3361 ccggttggag atgggttccc cgaccaaggc ggtcatcacc agcggcgaca caaggaccaa
     3421 tggcagcact tatccatagg ctggctgggc atcgcgttgc tcctcggttt gctctgcggt
     3481 tgtcgctcta cgcccaaggc ggaccctgag gtcgcctatc ggcaggcgcg cttgaccatc
     3541 gttcaaggcg acctgatcaa ggcgcaacag gaagctaaag agggatacgc tcgctattcc
     3601 cagcagagcg ctcagtgggc gtggaagttt cgtatcctcg aagccgaaat tctcacatac
     3661 agaaggctca accaggaagc gctctcgacg ctgaactcac gattgcccgc tgatctcgcc
     3721 agcggcgacg aagccatcca aaaaaacatg gtagaaggct tggcgctggc gcgcttgggc
     3781 cgccaggacg aagccaacga acgcttggac aaggccgacc aactctgcca actcagtcac
     3841 tcccacgttg atgggcagtt agcccagatc cacggtgtga tcgagtttgg ccgcggcgat
     3901 gtggctcagt ctgaggctta ctttcggcaa gtcctgcgaa tggctcgtga ggaaaaagac
     3961 caacttctcg aagccacggc cctgatgaac cttgggctag ctgcgttgaa gcaaggtcac
     4021 tacgacaatg caatcttgtg gtcgacggac ggctatgagg ctgcccgcaa cttgggagcg
     4081 cgcttcgccg aacagaaggc cctgggcaat ctgggctggg cctattaccg catgggcgac
     4141 cgtgagaaat ccctagtcta cttccaacaa gccgccgagg aagcgcgcaa gctggatgcg
     4201 aagaatgact tggcgaaatg gatccgagca ctggggatga tttcccaaga gatgaaccag
     4261 accagcaccg cagaaactta ctacaagcag tcgttgtcgc tggcccagca atccgacgac
     4321 aaagaagatc tggtggatgt gatgacagcg cttgcggctg tgtccgttga gcagcagaag
     4381 tgggagcagg cgaaccgcta cacccagcag gcgatgagcc tttgtcgctc ccggggcgat
     4441 cgcgagggag aactcgaggc ctcgctgatc atggggaaga ttgccctgca tcagaacgac
     4501 gcagctcgcg ctgtccagct atttagtgag gttgcggctg atcctcagaa cgaggcgccg
     4561 ttgaaatggg aggcccagga agacctggcg agactctatg aggatgaaaa gcgtccggcg
     4621 gatgcgtccc ggcaatacaa actctcgttg gcaacattcg aaagcacgcg cgcctcggtt
     4681 aaccaggaag aactcaggct accgtttctc gccaacagcg cgcaccttca cgatgactat
     4741 attcattttc tggtgaatca gggctcggag ctagaggcac tacaggttgc cgactatagc
     4801 cgcgcgcaga ctctcgccga aggacttaaa tcagggaaca gcaaagacag tgcgaagcct
     4861 actggaatag gcggtccgca gaagctggct tcgcaggtta acgccactat cttgttctat
     4921 tggctgggac cgagatactc ctatttgtgg atcgttactc cggatcacgt aaagctttcc
     4981 ccactgccat cggcagaagt tcttaacgcg ttagtcggta actaccgcaa ggatatacaa
     5041 gggcccagag acgccttaca agctgccaat cagaatggct ttgaactgta caagcttctg
     5101 gtcgcgccgg cgagccagga aatcgcaaac acgtcgctta cgattgttat ctcggacggt
     5161 gcgctgaacg acctgaattt tgaaaccctg ctcgtccccg ggcccaatcc acactacctg
     5221 attgacgatg ccaccattgt gaacgccact tcgctccggc tgctgaagac ctccgcccaa
     5281 gcccccatcg cgagaccgaa gctcctgctg attggggatc ccgtttcgcc gaatcgtgac
     5341 tacgccgaat tggccgacgc tggggccgag gtacgcagca tcgagggcca ttttgctccg
     5401 actgaacgaa cggttctcac ccggggcgag gcgacgagcc gggcctattt cggtagcaat
     5461 ccgcagcagt tctcctatat acattttgtc gcccatggga cggccagcag acttagccct
     5521 ctggattcgg ctgtcgtctt gtcgaagtcg gcggcggaag acgattcatt taagctctat
     5581 gctcgcgaca tcattcgccg gcctattcag gcggacctcg tcaccatctc aacctgctat
     5641 ggtgcgggag cgcgtgccta taccggcgaa ggtttagtgg gtctctcctg ggcctttctg
     5701 cgcgccggcg cacacaacgt gattgctgcg ctttggcaag ccagtgattc cgcaacgccg
     5761 cagctgatgg atggcttcta caaggagtta gaaaaaggtc atagtcccga tgtagctctt
     5821 cgatcagcga aactgtctct tctgcattcg gacagcgtgt tccgcaagcc tctgtattgg
     5881 gctccctttc aaatttatag cgatcagata aagccgcgtg cttcgggaaa aacgcctcag
     5941 caccatcgct gaggcaggct gactgtgatc agtgggaaac aatctaagac gcgacgcatg
     6001 ccaggtcgtt gtcgattagg aaaggggagc atctctgctc ccctcttgcc tgggctgagc
     6061 tatgaaccgt atattccgcc gctgcttccg cgcttcctgc gggcgaatac gcttacttcc
     6121 tgctgaggac ctgcgtctct aaccagcccg catccgaccc gaagtgcgat cccgcgggaa
     6181 cggtcccagc gtaaccatcc cgcattccat tcgcacgatc cgggagcttc agaacagatt
     6241 tgggcgtccg tttgcgcttg ctctttgcca tcggtacggc ctccaatcgc cgtggcaaac
     6301 atcttgccat ctcggtctat cgcacgctgg cgcgtttaac acgactagtc tagtccgcag
     6361 ctcagcgagg ataataggac gttcattttg ccgcaagcag ccgccgctca ggtgtcatta
     6421 gcaatcttgc caaccgatac tcttggccct tgccaggcga aggaaggcct cgttgataac
     6481 agcggtcggc tgacgggtat gacctgagcg ttccttgcga aaacaattgc tcgcaatctt
     6541 tcgcagatcc ggcatgagtg cttcaaagag ctgcgactcc gccagcggat cgttgttctg
     6601 ccatcgtttc agcagtactg tgatttcccc agggttgtct ttcgtctatc accccaactc
     6661 aaatgcgcca gactgcagcc cccaactgcg aaatcggcgg agagctctgc tcaaagtgtg
     6721 tgccaggact atccggagcc ggttggtcgt cgcgagattc tccgtgaaag agatagttgc
     6781 gcgtgggacc gaatcgtttc ataccaaggc ggtgaagttg aagaatatat cgcgcaaatt
     6841 cctgcgtgat cgctgaaacg aaaaccagaa gtcattcact accctgtata accagcgcat
     6901 tgtgccgttc acgagagcgg gaaaagcgag cacaaccggc cagccgctct cgaacaggga
     6961 tccaggaggc gggacaagca tcccgattcc ggctagagaa acaggtcccg aaatcacaac
     7021 ccggcgccgc acgacttaca ccgacgcccc aacctacgac cataaggagg tcaacatgac
     7081 gccgcaaatc agcaaacagc acagcaatgt agtcgcagcc caacccccaa ccacgttgcc
     7141 caaggaggat cgtatgaaag caaagaggca tctaatgttt ttcgccttga tgttcgctct
     7201 agtcgcattg aatgggcggc tcaatgcgca attcccggtg aaagagtttg ttccgttcaa
     7261 tagttttgta gaggagacaa aagcggccaa cacgagcgat tataccgcac gctcaacgag
     7321 caaggtaaga gacgccgcag ccttcgagga aatgcgtcaa catatcctca acctttacca
     7381 gggagttcag gtgagccata gctttgtcgt cgagtcgcaa acgtacgact gcatcgcgat
     7441 cgaggagcag ccctcggtcc gacttctcgg tttgaagggc atcgctgccc cgccgccagc
     7501 gtcagcagga ccggcggaac gcgttggggc gcctgataac agtcgcgcgc aatcgggaca
     7561 aacggcagtc tcttccattt ttccggctca gggtgtggat cgcttcggga acacgatcgg
     7621 ctgccaggcc aataccggtc ccgtagggcg gaccaccctc gaacagatga cccgattccc
     7681 gacgctaaag g
//
LOCUS       DASRKK010000697        13069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1145326, whole genome shotgun sequence.
ACCESSION   DASRKK010000697 DASRKK010000000
VERSION     DASRKK010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145326"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFC15_16915"
     CDS             complement(<1..813)
                     /locus_tag="VFC15_16915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM11892.1"
                     /translation="MTTSTVRHPERLAGKLLAVLVLLGISVFINYIDRGNLSIAAPML
                     KDELLISPTQLGILLSAFFWTYACLQPITGWLVDRVNVNWVLAGGFFLWSAATAATGL
                     VRTFSMLFALRLLLGIGESVAYPSYSKIIALNFPEEHRGLANSVISAGMVLGPGIGML
                     LGGTLMEKFGWRPFFFVLGLVSLLWLVPWVKWMPKARVSSTPGTTGAPSLAEFLQLRS
                     AWGSCVGLFSGNYVSYFLITWLPFYLVRERHFTMSTMARIGGLAYLLGACSST"
     gene            complement(1197..1271)
                     /locus_tag="VFC15_16920"
     tRNA            complement(1197..1271)
                     /locus_tag="VFC15_16920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1236..1238),aa:Glu,seq:ctc)
     gene            1457..2302
                     /locus_tag="VFC15_16925"
     CDS             1457..2302
                     /locus_tag="VFC15_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11893.1"
                     /translation="MPSSTTLRARVVSALGEQLSSTLLLRERAAPLTVPTGLAALDSL
                     TGGWPRGALSEINGPASSGRTGVMLAALAGATRREEACALVDASDNFDPASGAAAGVD
                     LDRLLWVRCGAPNCTSLRKDQRRGALGRLEQVLKVTDLLLQGGGFGMVVLDLGDIPTE
                     SVRRIPLTSWFRFRRAVEPTATVLLVVEREACAKTCASLVVRLQRTAVCARDSAATTH
                     PKSQGWKVLGLETSAISSIPSVNVVNHAALLRGLQLRAEVVRSWAQRKPMRSVGTNFE
                     LRAAW"
     gene            2328..4136
                     /locus_tag="VFC15_16930"
     CDS             2328..4136
                     /locus_tag="VFC15_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZM11894.1"
                     /translation="MFACIYVPDFPVAAIVRAEPLLCDRAVAVLEGKPPLVRVVSLNE
                     KARLLGMEVGMTKLQAAIFAGPETEGAVPAQPEPRKKTSQIEISSRRAKVQPKLTAAV
                     LRQRSPVQEESSHAALLDIAHAFTPQVEDTYPDRLLIDLDGLERLYGSAATMARELAS
                     RVSAVGLECNIGMATNPEAAMHAACGFSGVTVIPAGEEAKRLGVLPLTVLLDSFDIAR
                     RAETETLTASRERAKLREQMLDTLERWGVRDFRRLALLPEHALKSRLGEAGARLQRLS
                     RGQGMRILVLCEPQAHFEEAMELESAVETVESLSFVMNRLLEQLCARLGARVLAVQEL
                     KLRLQLERRVADEQTTTVQELAGVGNASTFSDPSDGNFYTRTLRLPVAMRDPKVFLKL
                     LQLDLAAHPPGAPVSKVWIAAEPARPRSAQQGLFLPITPEAEKLEITLARITGVVGER
                     RAGIARLLDTCRPDAFRMERFACQQESNEPNITLTLSDTADIPPIALRVFRPVRQLRM
                     QLAEGHPCKLAALTRIADRDELQGKILWSAGPWRSSGDWWAEQATVKNADEEAHVWDR
                     EEWDIALANDDGVSVALYRIYRDAVTGQWFADASYD"
     gene            4145..7561
                     /locus_tag="VFC15_16935"
     CDS             4145..7561
                     /locus_tag="VFC15_16935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZM11895.1"
                     /translation="MYIELHTRSAFSFLEGASVPEEMAEMCHSHQMPAMALLDRDGVY
                     GAARFHLAMKRLGRKAHIGVEITSLLPHVTLPDNPVLPRVSLLCASREGYQNLCRLVT
                     RMKLRAPKYPASLPRRAKPEATEDLLVSSQAVASPDDLAEFSSGLICMTGGEEGPLSH
                     ALAQGGLEEGRRILEQLVTIYGQNNVYVELQRHYSRAQESRNQAAIELARSLKLPLLA
                     TQGAQYAKPGAREILDVFTCIRHHRNLDTAGRLLSRNSERHIKSPQEMLRLFADLPEA
                     TANTLELSSRLEFTLNDLGYKFPSYPVPGGGPMSAFLRERTLEGARVRYQRPGQSQLW
                     QRARRQIERELTLIERLELEGYFLIVWDIVRFCREQGILVQGRGSAANSAVCYSLGIT
                     AVDPIGMELLFERFLSEERGEWPDIDLDLPSGDQRERAIQYVYERYGKLGAAMTANVI
                     TYRGRSAAREVGKVLGFDEETLDRLAGLVSSWEYKDPGDTLERQFRDAGFDLQHPRIR
                     KFFELCVAVQDLPRHLGQHSGGMVICQGQLDSVVPLEPATMPGRVVVQWDKEDCADMG
                     IIKVDLLGLGMMAALAECITLIHDHYKEDVDLAMLPADDPTVYDALQKADTVGMFQIE
                     SRAQMSCLPRLHPMRFYDIVVQVAIIRPGPIVGKMVNPFLLRRQGRQPVVYPHPSLEP
                     VLERTLGVPLFQEQLLRMAMIAAGFTGGQAEELRRAMGFKRSELRMRDIEVKLREGME
                     RNGLSREAQEEIILSITSFALYGFPESHAASFALIAYASAYLKCHYLAAFTAALLNNQ
                     PMGFYSPATIVKDAQRHGLRVKPVDITRSEWLCTLEVEASPTMWDSPSQEGAADKGEK
                     QIPQWLKPLRDDNNKELHPGLALRMGFRYAKGLREETAQAIVWARGQGAFESIADLAH
                     RVPELRRNELVLLASIGALNPIGCATAKDVAEQIDAPRTKQRLHRRDALWQVERAARF
                     AGPLLEGIPEIDTASPLFPMTKEERLVADFHGTGLTVGPHPLAYRRDELRRARVLSAQ
                     ELTQVAQGRYVRTAGCVIARQRPGTAKGFVFLSLEDETGISNAILTPDIMDRHRVVIT
                     SENFLLLEGILQNQEGVVSVRVERVAPLREVYAPDAIDITDAEVRSHDFH"
     gene            complement(8188..8862)
                     /locus_tag="VFC15_16940"
     CDS             complement(8188..8862)
                     /locus_tag="VFC15_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11896.1"
                     /translation="MKFRNGFSILLAVAFALTMSVAAFAQAPCNFPGLIYCQPWDGGG
                     NLFASQNDTVSGFGNFATVYDNFTLKQGYDIRSFHWVGGYFNPPNQGPITAWTLTFYN
                     DNAGTPGGAIGAGVFGGTANETFLGYAGGFPIYEYSLYFHSFDIGPGTYWASVVPDLG
                     FPPQWGWSTGTGGDGVAYQCFFGGCGPIAVDMAFSLDTPEPGTLIMLGTGILGLAGAL
                     RRKINL"
     gene            complement(9210..10100)
                     /locus_tag="VFC15_16945"
     CDS             complement(9210..10100)
                     /locus_tag="VFC15_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZM11897.1"
                     /translation="MAEIRTIAVIGAGIMGRGIAHAAALGGYRTILEDILPTTLRKAE
                     TEIRTNLDKAVELGKVTPEDADQAFKRLEYASSVEQASREADLVIEAVPEEMESKLEI
                     FTLLDKVCRPNTIIASNTSSLSITEISSVTYRAPKCVGMHFFNPVHKMKLLEIVRALE
                     TDDETLAAAVDVGQHMGKEVVVIRESPGFITSRINALIGNEAFYMLQEGVASAEDIDQ
                     AIKLGLNHPMGPFEMVDLVGLDTRLHILEYLHKTLGEKFRPAPLLVQYVKAGRLGRKA
                     GRGVYAYPGHEPGRPDSSGD"
     gene            complement(10106..10291)
                     /locus_tag="VFC15_16950"
     CDS             complement(10106..10291)
                     /locus_tag="VFC15_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11898.1"
                     /translation="MGASGELIRLMNYVDDIAATLRRVSGSLHLLADDEKKRLADYMR
                     KSDPNFVSILEQLEPKA"
     gene            complement(10806..12365)
                     /gene="malQ"
                     /locus_tag="VFC15_16955"
     CDS             complement(10806..12365)
                     /gene="malQ"
                     /locus_tag="VFC15_16955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZM11899.1"
                     /translation="MFTKRSSGILMHPTSLPSRGGIGDLGPAAYAFVDWLAAAQQTLW
                     QILPFGPVGYGNSPYSCTSAFAGNVLMISLERFVERGYLDPSRLSNLPDANAAVDFES
                     VRALKLPLLREAARNFLHGTYPLARKRYDAFCSENEWWLKDYALFSVLREHFGDHPWN
                     SWPEEIARRTPEALLQLRSDLKDQLALECFLQFAFFEQWRALRAYAADRGIRIIGDVA
                     IFVSYDSADVWTHPDIFRLHEDLSPEFVAGVPPDAFSETGQRWGNPLYNWDALKARGY
                     DWWIQRMRWAVETCDIVRLDHFRGYEACWEIPADEPTAVNGHWTAGPNEDLFKTLRNE
                     LGKLPFIAEDLGYITPEVRALRKKLDIPGMKVMQFGFGDRGAHIYLPHMFTIDSVVYT
                     GTHDNNTTVGWWDSDASETEKRCALEYLGKVDDGINWAFIRAAFSSVAILAVIPVQDV
                     LGLPGDARMNIPSKPGGNWSWRLAHGALTPELADKLAVLTVVTDRDASVKAPATSGGS
                     QGTGMGADFAA"
     gene            complement(12501..>13069)
                     /locus_tag="VFC15_16960"
     CDS             complement(12501..>13069)
                     /locus_tag="VFC15_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11900.1"
                     /translation="PATAQTPQGFAIYQVTEIEAPQTPTFEQIKPQVEEQFKAQRAQS
                     LLAQRTQELSDRAHAGHDLHKAAKELGATVKTSDLVNATAQVPDVGAMSGAANVAFTL
                     AVGEISGPLQGGATSGVVLAVLEKQEPSAAELKQNWDRAKETLLDQKRQTLEGLYVQN
                     LRDKLEKEGKIKVNKKEMERLSHLSEGS"
BASE COUNT         2778 a         3607 c         3954 g         2730 t
ORIGIN      
        1 ggtcgaggaa caagccccga ggagataagc gaggcctccg atcctggcca tggtcgacat
       61 ggtgaaatgc cgctcgcgaa ccaggtagaa gggcagccac gtgatcagaa aatagctgac
      121 gtagttgccg ctgaagagcc cgacacagct tccccatgcg gaacgcagct gcaagaactc
      181 agcaagactc ggggcgccgg tcgtgcctgg tgtactagaa actcgggcct tgggcatcca
      241 cttgacccac ggcaccagcc agagcaaact gaccagtccg agcacaaaaa agaagggacg
      301 ccagccaaac ttttccatca gagtgccacc cagcagcatg cctattcctg gtcccaatac
      361 cattcctgcg gagatgaccg agttggcgag accgcgatgt tcttcaggaa agttcagcgc
      421 aatgatcttc gaatacgaag gatatgcgac cgactctcca atgccgagta gcaaacgcaa
      481 cgcgaagagc attgagaacg tgcgcaccag gccggttgcg gcggtcgccg cagaccatag
      541 aaagaatcct ccggcaagga cccagttgac gttcacccgg tctaccagcc agccggttat
      601 tggctgcaaa catgcgtagg tccagaagaa cgccgagagc agaattccca attgcgttgg
      661 cgagattagc agctcgtcct tcagcatcgg agcggcgatg gagaggttgc cgcgatcgat
      721 gtagttgatg aacacagaaa tgcccagcaa cacaaggacg gccagcagtt tgcctgcgag
      781 tcgctcgggg tggcgcacgg tcgacgttgt cataataagg agtcgtgatt gtctgttgcc
      841 acggagagtt ttgcaacctt cttcgttgga acgatgtgtc gccgatttgc gacctgggcc
      901 gcgacacgct catttctgcg aaattattgt cgtgaaatcg ccatgtcact cgcaatccgt
      961 agcggaagta caggccccta aaggcacaga atgatttgcc ctcgaagttt caattgcgcc
     1021 aggtttcgcg actgataact atatcgttaa taggcacgtt tatagcgtct ttggctttaa
     1081 cggatcggga ggcgatgaac aagcggaatg aggcaatgca gtaaattgcc acaaaattgc
     1141 cacaatcgag tgaaaaccgt atcgggagac ttggtgctaa gtgcaatgga atcagttggt
     1201 agcgctaccg ggaatcgaac ccgggtttga agattgagaa tcttccgtcc taacccctag
     1261 acgatagcgc cacgctgggg gaacttcctg attataacaa actcggcaat gtggccgaag
     1321 ccgctccaga agcggctttg cgaggggttg acaagcctcg cgtcagggcg cagaatccta
     1381 tctgcgtagg cgaataaaaa gcgaaagtaa aactctcgac actcgcccgt catcagtccg
     1441 cacctgaggt cttcccatgc cgtcatccac cacgcttcgt gcccgcgtcg tgtcagcact
     1501 cggcgaacag ctgtcgtcca ccttattact gcgtgaaaga gctgcgcccc tgacggtccc
     1561 caccggactg gctgcgctcg actcgcttac cggaggatgg ccgcgcggag cgttaagcga
     1621 aatcaacgga ccggcttcct cgggacgcac cggagtaatg ctggcagcgc tggccggagc
     1681 cacgcggcgt gaggaagcct gcgccctggt ggacgccagc gacaatttcg atccggcttc
     1741 gggcgcggcg gcaggcgtgg acctagaccg attgttgtgg gtgcgttgcg gagcacctaa
     1801 ttgcacttcc ttacgcaaag accagcgacg aggcgcactc ggacgcctgg aacaagtgct
     1861 gaaagtgact gacctgctgc tgcagggcgg cggctttggc atggtcgtgc ttgatctcgg
     1921 ggacattccc acggaaagcg tgcgccggat tccgcttacc tcgtggtttc gttttcgccg
     1981 ggccgtagag ccaactgcaa ccgtgttgct tgtagtggag cgggaggcgt gcgcaaaaac
     2041 ctgcgcctcg ctggtggtgc ggctgcaacg aacggcagtg tgcgctcgcg attcggccgc
     2101 gacaactcat ccaaaatcgc agggatggaa agttttgggg ctggagacat cggcaatttc
     2161 atccattcca tcggtgaatg tggtgaatca tgcggccctg ttgcgtggcc tgcaattgcg
     2221 cgccgaggtg gtgcgttcgt gggcgcaacg caagccgatg cgctcggtgg gcaccaactt
     2281 cgagctgcgc gctgcctggt aaacactggt cttgcctgtg ggtctgcatg tttgcatgta
     2341 tctatgttcc cgattttccg gtggcggcca tcgtacgtgc tgaaccgttg ctatgcgacc
     2401 gtgccgtcgc cgtgctcgag ggcaagcctc cgttggtgcg cgtggtgtcg ctgaacgaga
     2461 aagctcgtct gctcggaatg gaggtaggga tgactaagct gcaagctgcg attttcgcgg
     2521 gccctgagac agaaggtgca gttcccgcac agcccgaacc caggaagaag acttctcaaa
     2581 tcgaaatttc gtcacgacgc gcaaaagtgc aaccgaagct taccgctgcg gttttgcggc
     2641 aacggtctcc ggtgcaggag gagtcgtcgc acgctgcgtt gctggatatc gcgcatgctt
     2701 ttaccccgca agtggaggac acatatcctg accgcttgct gattgacctc gatggactgg
     2761 agcggctcta tggatcggct gcgacgatgg cgcgggagct ggcttcgcgg gtgagcgctg
     2821 ttggactcga atgcaacatc ggcatggcca ccaatccgga ggctgcgatg catgctgctt
     2881 gcgggttcag tggagtcacc gtgattcccg caggagagga agccaaacgg ctgggagttc
     2941 tgccgctgac tgtactgctc gattcgtttg acatcgcacg ccgcgcggaa actgaaactt
     3001 tgaccgcctc acgcgaacgc gcaaagctac gggagcagat gctcgacact ctggagcgct
     3061 ggggagtgcg cgatttccgc aggctggcct tgctacccga gcatgccctg aaatcacggt
     3121 tgggcgaagc aggagcgcgg ctgcaacggc tgtcccgtgg acaggggatg cggatcctgg
     3181 tgttgtgtga gcctcaggcc cattttgaag aagcaatgga attggagtcg gcggtcgaaa
     3241 ctgtcgagtc gctgtcgttc gtgatgaacc gtctgctgga gcagttatgt gcaaggctgg
     3301 gagcgcgggt cctcgcggtg caggaattga agttgcgctt gcaactggaa cggcgcgttg
     3361 cggacgagca aaccacgact gtgcaagaac tggctggcgt gggtaatgcg tctaccttct
     3421 cggatccgag cgatggcaat ttctatacgc gcaccttgcg cctgccggtg gccatgcgcg
     3481 atcctaaggt gtttctgaag ctgctgcaac tggatctcgc cgcgcatcct ccaggagcgc
     3541 cggtctctaa agtatggatt gcagctgaac cggcccgtcc ccgttcggcc cagcagggct
     3601 tgttcttgcc catcactccc gaggctgaaa agctggagat tacgctggcc cgcatcacgg
     3661 gcgtggtggg cgaacgccgc gcgggaattg cgaggttgct ggacacttgc cgtcccgacg
     3721 cttttcgcat ggagcgtttt gcctgccagc aggagtcaaa cgagccgaac ataactctga
     3781 cattgtcaga taccgctgac atcccgccga ttgccttgcg tgtgttccgt cctgtgcgcc
     3841 aactccgcat gcaactggca gagggacatc catgcaagtt ggcggcgttg acccgaatcg
     3901 cggatcgaga tgagttgcag gggaaaatct tatggtcggc gggaccatgg cgctcttccg
     3961 gagactggtg ggcggaacaa gcgacagtaa agaacgccga cgaagaagcg cacgtttggg
     4021 accgcgaaga atgggacatc gcgctcgcca atgacgacgg cgtcagcgtt gcgctttacc
     4081 ggatttatcg tgatgcagtc acaggacagt ggtttgccga tgccagctac gactgacgac
     4141 tcgcatgtac atagaactgc atactcggtc ggcattcagc ttcctcgaag gagcttcggt
     4201 tcccgaggag atggctgaga tgtgccactc gcaccaaatg ccggctatgg cgctgcttga
     4261 tcgcgacggt gtttatggcg ccgcgcgctt tcatctggcc atgaaaagac ttggccggaa
     4321 ggcgcatatc ggggtggaga tcacttccct gctaccgcat gtgacgctcc cggacaatcc
     4381 cgtgcttccg cgcgtgtctc tgctgtgcgc ttcacgcgag ggatatcaga acctttgccg
     4441 gctggtaacg cgtatgaaat tgcgcgcgcc gaaatatccg gcctctctgc ctcgccgcgc
     4501 aaaacctgaa gcaaccgagg atctgttggt aagttcgcag gctgtcgcct ctcctgacga
     4561 tctcgccgag ttttcttccg gactgatttg catgaccggc ggagaggaag gaccgctgtc
     4621 ccatgcgctc gcgcaaggcg gcctcgaaga gggacgccgc atacttgaac aactcgtgac
     4681 gatctacggc cagaacaatg tgtatgtgga attgcaacgc cattacagcc gggcgcagga
     4741 gtcgcgaaac caggcagcca tcgagttggc tcgcagtttg aagctgccat tgctggcgac
     4801 tcaaggagca caatacgcca agcccggggc acgcgagatc ctcgatgtgt ttacctgcat
     4861 ccggcatcat cgcaacctcg acacggctgg acggctgttg tcgcgcaata gcgagcggca
     4921 tatcaagtcg ccgcaagaga tgctgcgctt gttcgccgat ctccctgagg ccaccgccaa
     4981 cacgctggaa ctgtcgtcgc gcctggagtt cacgctcaac gatcttggtt acaagttccc
     5041 cagctaccct gtgcccgggg gtgggccgat gagcgcgttt ttgcgggagc gcacgctgga
     5101 gggagcacgc gtgcgctatc agcgtcccgg gcaaagccaa ctgtggcagc gggcgcggag
     5161 gcagatcgag cgcgaactga cgctcatcga gcggcttgaa ctcgaaggat attttctgat
     5221 cgtttgggac atcgtccgct tctgccggga gcaaggcatc ctggtgcagg gacgcgggtc
     5281 cgcggccaac agcgctgtct gctattcgct ggggattacg gcagtcgatc ccattgggat
     5341 ggagctgctg ttcgagcggt tcctctcgga agagcgtggc gagtggcccg atatcgatct
     5401 tgaccttccc agcggcgacc agcgcgagcg ggcgatccag tacgtctatg agcgatatgg
     5461 caagctgggc gcggccatga cggccaatgt cattacctat cgcgggcggt cggctgcacg
     5521 cgaggtgggc aaggtgctgg gcttcgatga agaaacgctg gaccgcctgg ctggcctggt
     5581 gagttcgtgg gagtacaagg accccggcga cacgctggag cggcagtttc gcgatgcggg
     5641 atttgatttg cagcatccgc gtattcgcaa gttcttcgag ctttgcgtag cggtgcaaga
     5701 cctgccgcgg catctgggac agcactccgg aggcatggtg atctgccagg ggcaactgga
     5761 ttccgtcgtg ccgctcgagc ctgcaaccat gcccggccgc gtggtggtgc agtgggacaa
     5821 ggaagattgc gccgacatgg ggatcatcaa ggtcgacctg ctggggctgg gcatgatggc
     5881 cgccctggcg gagtgcatca cgctgattca cgatcactat aaagaagatg tagatctggc
     5941 catgctgcct gcggatgatc cgacggttta tgacgcattg cagaaggcag acacggtggg
     6001 catgtttcag atcgaaagcc gggcgcaaat gtcgtgcctg ccgcgtctgc atcccatgcg
     6061 cttctacgac attgtggtac aggtggccat catccgtccg gggcccatcg tgggcaagat
     6121 ggtgaatccg tttctcctgc ggaggcaggg acgccagcct gtggtgtatc cgcatccttc
     6181 gctggagccg gtgctggaac gcacgcttgg ggtaccgctg tttcaggagc agctcttgcg
     6241 catggccatg atcgctgccg gattcacagg cggacaggcg gaagagttgc gtcgcgcgat
     6301 gggcttcaag cgcagcgaat tgcgcatgcg cgacatcgag gtcaagctgc gcgaaggcat
     6361 ggagcgcaac ggcctttcgc gcgaggcaca ggaggagatc attctctcca tcacttcgtt
     6421 tgcgctgtat ggatttcctg aatcgcatgc tgccagcttt gctctcattg cttatgccag
     6481 cgcctatctg aagtgccatt acctggcggc ttttactgcg gcgctgctca acaatcagcc
     6541 gatgggattc tattcgccag ccaccatcgt gaaagatgcg cagcggcatg gcttgcgggt
     6601 gaagccggtg gacatcacgc gatcggaatg gctgtgcacg ctggaggtgg aagcgagtcc
     6661 cacaatgtgg gatagcccat cccaggaagg ggctgctgac aaaggcgaaa agcagatccc
     6721 tcagtggctg aagccacttc gtgatgacaa caataaagag ctccacccag gcttggcttt
     6781 gaggatgggt tttcgttacg ccaaggggtt gcgggaagag acggcgcagg ccattgtgtg
     6841 ggcgcgcggt cagggggcct tcgagtcgat tgccgatctt gcccatcggg ttcccgaatt
     6901 gcggcgcaac gagttggtgc tgctggcttc gattggagcg ctgaatccga ttggctgtgc
     6961 gactgcgaaa gatgttgctg aacagattga tgcgcctcgc accaagcaac gattgcatcg
     7021 ccgtgatgct ttatggcagg tggaacgggc tgcacgattc gcggggccat tgctggaggg
     7081 cattccggag atcgatactg cctcgccgct gtttcccatg acgaaggaag aacggctggt
     7141 tgctgatttt cacggcaccg gactgacggt ggggccacat cctctggcct atcgacggga
     7201 tgaattgcgc cgcgcccgag tgttgtccgc gcaggaattg acgcaggtcg cgcaaggccg
     7261 ctacgtgcgt acggccggat gcgtgattgc aaggcaacgg ccgggcactg caaagggctt
     7321 tgttttcctg agcctggaag atgaaactgg aatcagcaac gcaattctca cgccggacat
     7381 catggaccgg caccgagtgg tgattacgtc ggagaacttt ttgctgctgg aggggattct
     7441 gcagaaccag gaaggggtgg tttcggtgcg ggtggagcga gttgctccgc tgcgagaggt
     7501 ttacgcgccc gatgctatcg atatcacgga tgcggaggtc aggtcgcacg attttcatta
     7561 gtgcgtttga gccgcagtgc gtggctaatc tgcaacaaat tacaaatatt actttattcg
     7621 tgttgcttgt tttcaatgac ttgggcagaa aattgttgca tgtcattgaa tcaaaatcta
     7681 gttatatcat ccatgtctgc cgtaaggact tatatctgca atttgttgca cattcactgg
     7741 attgggcaga ggtcgagttt tcaaagaacg ggagttacag gactgatccg aaccgttcga
     7801 cgccgccgga atcgtgactc cgtcgcgcgt cgagttctgc tcccaggcga aacatcccag
     7861 tttcagagat acggcaaata ggagtgggag tcacgcaaaa taagcagtgg gaagaaacga
     7921 aagtaacctg gttccttggc gaaatatgag gcacagcgtc gatttctcgc acagcccaca
     7981 cactcttatc gacgtggcgc cgtcgggccg acgtgactgt tatcgattgg ctacgatacg
     8041 gcctgactac gacagctacc ttgctttgag ttgaggtatc gcaccaattg gctggccact
     8101 ctggtatcaa tcagagatta ggctcggtga ccgggcagaa gaaagccggg gagcgattcc
     8161 ccggctgcct acacacagaa gtcaaagcta caagttgatt ttgcggcgca gcgcaccagc
     8221 caagccgaga attccggttc ccagcatgat cagcgtgccc ggttccggtg tatcgagcga
     8281 gaaggccata tcgaccgcga tcggaccgca gcctccgaag aagcactgat atgccacccc
     8341 atcgccgcca gtacccgtcg accagcccca ctgcggtggg aaaccgaggt cgggaacgac
     8401 cgacgcccaa taggtgcccg gaccgatgtc gaagctatgg aaatacagcg agtactcgta
     8461 gattgggaaa ccaccggcgt aaccaaggaa cgtctcgtta gcggtgccgc cgaacactcc
     8521 ggctccaatc gcacctccgg gtgtcccagc gttgtcgttg taaaaagtca acgtccaggc
     8581 ggtaatcggg ccctggttgg gagggttgaa atatccgcca acccagtgga aactgcgaat
     8641 gtcataccct tgcttcaggg tgaagttgtc atagacggtg gcgaaattgc caaagccgct
     8701 tacagtatcg ttctgggaag caaacaagtt gccgccgcca tcccatggct ggcaatagat
     8761 taagcctgga aagttgcatg gggcctgtgc gaatgcagcg acgctcatag tcagcgcaaa
     8821 tgctacggcc aacagaatgg agaagccatt gcgaaacttc atttgttact tcttgtgcgg
     8881 atggagcgag atgcatgtta gatgattagc tgtggttgaa cctccgacat aggaaatatg
     8941 gtggaaagtc tatttcaact tccaactgcc gattcaccaa tgttggacga tcgctgtgct
     9001 tgggggagtc acaacaagcc tagctccggc gaagaacggc ggcgcaagaa ccgggcagaa
     9061 aatttggtgg attcccaaaa cggaatcgaa cggtccacgc cctgatttct gcgtttctag
     9121 gtgcttcggt gttcgtcgaa caaccgtagt ggtcgtgaac tacccatggt gtaagtcatt
     9181 cgcaccgagc gatggcgata ggctatttct caatcaccgg aagaatcggg tctgccgggc
     9241 tcgtgtccgg gataggcgta cacaccgcgg ccggctttgc gacccaggcg gccggctttc
     9301 acgtattgca ccagaagagg cgcgggacgg aatttttcgc cgagggtttt gtggaggtac
     9361 tcgagaatat gaaggcgggt atccaatccc acgaggtcaa ccatttcaaa tggtcccatc
     9421 gggtggttca agcctagctt gattgcctga tcgatatctt ccgcggaggc gacgccttcc
     9481 tgcaacatgt aaaacgcttc gttgccgatc agggcattga tgcggctggt gatgaagccg
     9541 ggggactccc tgatcaccac cacttccttg cccatgtgct ggccgacatc aacggcagca
     9601 gctagcgtct cgtcgtcggt ttcaagggca cgcacaatct caagcaattt cattttgtga
     9661 actggattga agaagtgcat gccgacgcac ttgggagcgc ggtaggtgac ggatgaaatc
     9721 tcggtgatgc tgagcgaaga agtgttggaa gcgatgatgg tgttggggcg gcagaccttg
     9781 tccaagagag tgaagatctc cagcttggac tccatctcct cgggcacggc ctcgatcacc
     9841 aggtcggctt ccctggacgc ctgttctacc gaagacgcgt actccaaccg tttgaacgcc
     9901 tgatcagcgt cttcgggtgt caccttccca agttcgaccg ccttgtcgag gttggtgcga
     9961 atctcagtct cagccttgcg cagcgttgtc ggcaggatgt cctcgaggat ggtgcggtac
    10021 ccacctaatg cggcagcgtg ggcgattccg cgccccatga ttccggcgcc gatgacggcg
    10081 atggtgcgta tctcggccat gcgggctaag ccttgggctc caactgctcg aggatggaga
    10141 caaagttggg atcggacttg cgcatatagt cggccagacg cttcttctcg tcgtcggcga
    10201 ggagatgcag gctgccgctg acacgccgaa gcgtcgccgc gatatcgtcc acgtaattca
    10261 tcaagcgaat tagttcacca ctggcaccca taagagtcct ttcaaataat gaagatgaga
    10321 aaccttactt acgtcagggg ctaaagagag ctctgccgtc cccgagggac tcgaatcgtc
    10381 tttccacttt acccagcact cctccttcgg ctgcgtgctg ggctatcatg tcgccgcccc
    10441 tgcggggctg attctctagt gcttcgcacc accgtcgcaa cccaaaccta attctcacgc
    10501 gacatctaaa gccaagacag cacgaccgaa caggctacgg taaagtcgtg aaagcagact
    10561 cccgcgggct gaggcccgcg agaaatgaca aaaatactga acttagacgc tggcctgaag
    10621 gccagctcta cccaaaatcg cttttttcag cagcctgtaa agtcggtcct ttcaaatatc
    10681 gcttcagcaa gcaaactgtt tcttcatcca gtcgttgtac acacgcacac agcctaagga
    10741 acggcctgat tctcattctc tgcgtcaccg gcaattaagt aaagaagcct caactcgttg
    10801 gcgaatcacg ctgcgaaatc cgcgcccata ccggtgcctt gcgaaccccc agaggtagca
    10861 ggagccttta cggatgcgtc ccggtcggtc actaccgtga ggaccgccaa cttgtcagcc
    10921 agctcgggcg ttaacgcacc atgggcaaga cgccatgacc agttgccgcc cggcttgcta
    10981 gggatattca tgcgagcgtc accgggaagt cccaatacat cctgcacagg aatgacggca
    11041 aggatcgcaa ccgaagagaa ggcggcacga atgaaagccc agttgatgcc gtcatctact
    11101 ttccccagat actcaagtgc acaccgcttt tccgtctcgc ttgcgtcgct gtcccaccaa
    11161 cccaccgtgg tgttgttgtc gtgagtcccg gtatagacga cggaatcgat ggtgaacata
    11221 tgcggcagat agatgtgcgc gccgcgatcg ccaaaaccga actgcatgac tttcatgccg
    11281 gggatgtcga gcttcttacg caaggcgcgg acctcaggcg tgatgtagcc aagatcttcc
    11341 gcgatgaacg gtagtttacc gagttcattg cggagcgttt tgaacaggtc ttcgttggga
    11401 ccagctgtcc aatgaccatt gacagcggtt ggttcatcag ccgggatttc ccagcaggct
    11461 tcgtaaccgc gaaagtggtc gagccgcaca atgtcgcagg tttccacggc ccagcgcatg
    11521 cgctggatcc accagtcgta gccgcgcgcc ttcagggcgt cccagttgta gagcggatta
    11581 ccccagcgtt gaccagtctc gctgaacgca tcgggcggaa cgccagccac gaattcgggc
    11641 gagaggtctt cgtgcaagcg gaagatgtca ggatgtgtcc agacatcggc gctgtcgtag
    11701 ctgacgaaaa tggcgacgtc gccgatgata cgtattccac ggtcagcggc ataggcccgc
    11761 agcgctctcc attgttcgaa gaaggcgaat tggaggaagc attccagcgc cagttggtct
    11821 ttcaagtcgg aacgcaactg cagcaacgcc tccggcgtgc ggcgggcaat ttcttcgggc
    11881 catgagttcc aggggtgatc gccgaaatgt tcgcgcaaca cgctgaagag cgcgtaatcc
    11941 ttcagccacc attcattttc agagcagaac gcatcgtaac gcttgcgggc caaggggtac
    12001 gtgccgtgca gaaaattgcg cgccgcctca cgcagtagcg gcagcttcag cgctcggaca
    12061 ctctcaaaat cgacggcggc attggcatcg ggaagattcg ataagcgaga cggatcaaga
    12121 taaccgcgct ccacaaaacg ttccagactg atcatcagca cgtttccggc aaatgccgaa
    12181 gtgcaggaat atggcgagtt gccgtagccc accggaccga acggaaggat ttgccagagt
    12241 gtctgctggg cagcggccag ccaatccacg aacgcatacg ccgcagggcc aagatcgcct
    12301 atccctccac gggagggcag ggacgtggga tgcatcagga tgccggatga gcgtttggtg
    12361 aacatggata tcgagtggag cgtaaatatg ttgagaaaga agccaggaga ttcgaatctt
    12421 caaatgctgg cttgcggccg tcagaaaaag atgggccagc gactcgcttc gctggccacg
    12481 catctccgag cgaaggattc ctaggagccc tcactcagat ggctcaggcg ctccatttct
    12541 ttcttgttga ccttgatctt gccttccttc tcgagcttgt cgcgcaagtt ctggacataa
    12601 agcccttcga gcgtctgccg cttttgatcg agcaaggttt ccttggcgcg atcccaattt
    12661 tgcttgagct cggcggctga cggttcctgc ttctccaaca cagctaacac cactccgctg
    12721 gtcgcaccac cctgcaatgg gccgctgatc tcgcccacgg ccagcgtaaa tgccacattg
    12781 gctgctccgc tcatggctcc gacgtcggga acctgcgctg tcgcgttgac caggtcgctg
    12841 gtcttcacgg ttgcgccgag ttccttcgcc gccttgtgca agtcgtgtcc ggcatgagca
    12901 cggtctgaaa gttcctgcgt tctctgcgcg agaagtgact gtgcgcgctg cgccttgaac
    12961 tgctcctcga cctgcggctt gatttgctca aacgtgggag tttgcggggc ctcgatctcg
    13021 gtgacctggt aaatcgcgaa tccctgtggc gtctgcgccg tggctggag
//
LOCUS       DASRKK010000698         8627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1048131, whole genome shotgun sequence.
ACCESSION   DASRKK010000698 DASRKK010000000
VERSION     DASRKK010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048131"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFC15_16965"
     CDS             <1..614
                     /locus_tag="VFC15_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZM11901.1"
                     /translation="PVGAIVEGKVRNLTDFGAFIEIEDGIDGLVHVSNLSWTKRVKHP
                     SEVLKKGDKVKAIVLAIEPDQRRLSLGVKQLQPDVWDTFFEQHRVGDVIHGKVLRLAS
                     FGAFVEIADGVEGLCHNSEAVDHSGTQIKLEPNQEFDFKVIKMNPAEKKVGLSIRAVG
                     EEATRADVEAYKGPAAAATTTSSGTGSTIGEFMSWKRASNENN"
     gene            complement(686..1075)
                     /locus_tag="VFC15_16970"
     CDS             complement(686..1075)
                     /locus_tag="VFC15_16970"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM11902.1"
                     /translation="MKKFLGLLVIATVSVGCMAAQLTIPMGGGSSEPTPKKIKPTSKM
                     LNGTVQDKSDQPIAGAVVYLKNMKTLAVKSFFAQKDGAYRFPQLALNTDYEVYAEKDG
                     KKSDVKTISQFDDRFSPTINLRIDLNK"
     gene            complement(1095..1535)
                     /locus_tag="VFC15_16975"
     CDS             complement(1095..1535)
                     /locus_tag="VFC15_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM11903.1"
                     /translation="MSSVPLWLFHCGGKATRSAVVLLLLAAAGSLMAAPLPSPAPDPQ
                     KNTVSKDCALIAGTVWGPDDHPLAGVPITIHSVTGKKAKWDLVSNQRGEFAQRVPPGR
                     QDYIIQADIKTPKGQPKPEITVQIEDNERKDVGLHLTQQGLAKP"
     gene            complement(1528..1722)
                     /locus_tag="VFC15_16980"
     CDS             complement(1528..1722)
                     /locus_tag="VFC15_16980"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11904.1"
                     /translation="MVICPECESDLDFEEDEVDEGEVVSCPECGTDFEVVAIEPLQLS
                     RVEDEVIDDAEEVTDEEEDE"
     gene            1861..3261
                     /locus_tag="VFC15_16985"
     CDS             1861..3261
                     /locus_tag="VFC15_16985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZM11905.1"
                     /translation="MPKMKMFLAGAIGLWLTLSAATVPACGQVDNIPVPPPDPLIVEA
                     LKAVSAQRIQQTIEKLVSFETRHTLSSDVPASSGKGINAAAEWIRSEFEAYSKACGGC
                     LEVKTDEFTQDPASRIPKPTKLTNVYAILRGSDPANASRMFLVTGHYDSRNTETNDAT
                     GASPGANDDGSGTAVSLECARVLSQHRFPATIVFVAVAGEEQGLYGSAHIAKMAKAQG
                     WDLEAVLNNDIVGGDRTPGSTTQDRRTVRVFSEGIPATAAEAELKLIRALGGENDGAS
                     RQLARCIAGVSKAYLPAKEFHPLVVFRRDRYLRGGDHISFNEQGFAAVRFTEYRENYD
                     HQHQNVRTENGIEYGDLPKFVDYDYIANVARVNAATLASLASAPAPPAKVRVLTKALQ
                     NDSTLVWEASPGGLATGYLVLWRDTGAPDWEHAQAVANVTQATVPMSKDNVVFAVQAM
                     DAKGHRSLPVTPAPER"
     gene            3297..4175
                     /locus_tag="VFC15_16990"
     CDS             3297..4175
                     /locus_tag="VFC15_16990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZM11906.1"
                     /translation="MRRGTTMSLSFPPFTRAVKQLLIANGAVFLLVAVIGAFTSFDAG
                     RWADLHFGLVPTLAVFHGWIWQFVTYAFLHAGLLHILFNMLALWMFGAQLEVDWGYSL
                     FMQFYFFCVIGAAITTAVVSFTGLLGASPLVPTVGASGGIYGILVAFGILHGDSEIML
                     FPIPFMIKARYFVIGLILLAMYGALSSAHSMGQSTAYMAHLGGGLFGYLFLKFVPRRG
                     IGFLSSERLYGLRNSYYRWKRKRAARKFEVYMRKHDDRGDYRHDDYFDENGNMRDPAA
                     RDSQKKNGDARPPWVN"
     gene            complement(4187..4486)
                     /locus_tag="VFC15_16995"
     CDS             complement(4187..4486)
                     /locus_tag="VFC15_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11907.1"
                     /translation="MASEVVSVAILEGLPGKEEELLTMLREFYAMMKSKGYSSDFLTR
                     DSDRPDRFIHLRRWKSEELRGEAQIDPDVHRYWQQLPSLCSIPTVYENLETMFES"
     gene            complement(4499..6055)
                     /gene="pyk"
                     /locus_tag="VFC15_17000"
     CDS             complement(4499..6055)
                     /gene="pyk"
                     /locus_tag="VFC15_17000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZM11908.1"
                     /translation="MAEIESTKSGGFEPSDDPFRSPNTSRRRAKIVCTIGPASNSDEM
                     IRDLMLLGMDVARLNFSHGTHEQHAVVIERLRKVARQLNRSICIMQDLQGPKIRTGRL
                     ASGAPVALKKGQTVTITPNDVAGTTEIISTTYQNLADDVKPGERILLSDGRIELVVQE
                     VRESEVVCEVLNGGMLGENQGINLPGTNVSIPSLTEKDLRDLEFGLKQGVDLVAISFV
                     RTVNDVVRAKNAIAAYRSDVGLIAKLEKPQAIENLDGILEAAVGVMVARGDLGVEVAP
                     EKVPLIQKFVIRRALDFRKPVITATQMLESMTDSPRPTRAEASDVANAVFDGTDAVML
                     SGETASGKYPREAVEMMARIVAETEAHSLNPLTPGHREARHLSVAETICESMAHAAKD
                     LKMRAIAVFTETATTARMLSKYRPAADIYAFSSSETVRNRMNALWGTTPLMCPPKLSV
                     ERMAEFAEEELRRLNVLSPGDVFGLVAGTQNTTGATNFMRLIQVRDDTEIPNQTAGSW
                     PRSLFNLVLV"
     gene            complement(6059..6937)
                     /locus_tag="VFC15_17005"
     CDS             complement(6059..6937)
                     /locus_tag="VFC15_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZM11909.1"
                     /translation="MSRFFNHLRLAFWRAFEHDAFGVAKGAAFSAILTFFPAILVLAS
                     ILSRSQSSGVFVREIFRAVGRVLPEGTNNAVTEYVTGAKPVQTQFLVWISVLTLWTAS
                     GVMISWMEGFRKAYQMPKIWGLVKERLIAFQLVVMAGFPMGFASFLVVFGNQIERWTI
                     FHAGHELTPYILGLWTAVRWIIATMTSIAVIGLVYHHGVPRTQPWHRVLPGSVVATVL
                     WVSVTVLFGWYLRRYANYGVIYGSVGTAMALLIWLYLVSMVVLIGAEFNALRYPRFLF
                     GAHSDFNSAQKRVAAL"
     gene            complement(7092..>8627)
                     /locus_tag="VFC15_17010"
     CDS             complement(7092..>8627)
                     /locus_tag="VFC15_17010"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11910.1"
                     /translation="CESKRGVCVMCYGRNLASGRLVELGEATGVIAAQSIGEPGTQLT
                     MRTFHIGGTASRVSEQSRLDAKNNGAVRFVNLITVRSKEGYLVAMNRNGSIAVVDEKG
                     REKERYAVVYGGKVKVEDGQQVSLGDVLVEWDPYTFAILTEIGGTVQFKDLQEGLTLH
                     EEVDEVTGLSRHVVMDSPDEKRQPAIVIKGAKGSKRYLMPSRAHLMILDGDEVTPGEV
                     LAKIPRETTKTKDITGGLPRVVELFEARKPRETAVISEIDGIVKFGDVAKGQRKIYVV
                     ADNGTEKEYQVPRGVHINVQEGERIKAGEPLMDGPLNPHDILAVLGEKELQAYLVNEI
                     QEVYRLQGVNISDKHIEVIVRQMMRWVKVEDVGDTSFLLEQQIDKFRFREENEKTIAN
                     GGKPATGRPLLLGITKASLSTDSFISAASFQETTRVLTEASIQGAVDHLRGLKENVIV
                     GRLIPAGTGMEYYRNVRLSEEMEQAAVKVQEEVTAAYEEAERALELLRHEGENEAEEL
                     TAE"
BASE COUNT         1717 a         2598 c         2503 g         1809 t
ORIGIN      
        1 tcccggtggg cgcgatcgtg gaaggcaaag tccgcaacct gaccgacttt ggcgcgttca
       61 tcgaaatcga agacggcatc gacggcctgg tccacgtgag caacctgagt tggacgaagc
      121 gcgtgaagca tccgtcggaa gtcctgaaga agggcgacaa ggtgaaggcc atcgtgcttg
      181 ccatcgaacc cgatcaacgc cggctttcgc tgggcgtgaa gcaactccag ccggatgtgt
      241 gggacacgtt cttcgagcag catcgcgtgg gcgacgtcat ccacggcaag gtgctgcggc
      301 tggccagctt tggagcgttc gtggaaatcg ccgatggagt cgaaggcctg tgccacaact
      361 ccgaggcggt agaccacagc ggcacgcaga tcaagcttga acccaatcag gagttcgact
      421 tcaaggtgat taagatgaac ccggcggaga agaaagtcgg actcagcatc cgggccgtgg
      481 gcgaagaggc cacacgcgcg gatgtagaag cctacaaggg accggccgcg gcggcgacga
      541 cgacttcatc aggtactgga tcgacgattg gtgaattcat gtcgtggaaa cgggccagca
      601 acgaaaacaa ctaagtttcg aagtagcgaa actctgaagg ccgccagcgc tggcggcctt
      661 ttgttatgcg aatgcagatc aacggttact tattcaggtc gatgcgcagg ttgatggtcg
      721 ggctaaagcg gtcgtcgaac tggctgattg tcttgacgtc gcttttcttg ccatcctttt
      781 cggcatagac ttcatagtcg gtgttcaggg ccagttgcgg gaagcgatac gcgccgtcct
      841 tttgtgcgaa gaaacttttc accgccaggg tcttcatgtt cttgagatag actaccgcac
      901 cggcgatcgg ctgatcgctc ttgtcctgca ccgtgccgtt cagcatctta gaggtgggct
      961 tgattttttt gggtgtcggt tcactcgacc cgccgcccat gggaatcgtg agttgcgcag
     1021 ccatgcaccc gacggaaacc gtcgcaatta cgagcaatcc caaaaacttc ttcacggaat
     1081 ctcctgccgg gagcctatgg ctttgccagt ccctgctggg ttagatgcaa accgacgtct
     1141 ttccgttcat tgtcttcgat ctggacggta atctccggct tcggctggcc tttgggggtc
     1201 ttgatgtcgg cctggatgat gtagtcctgc ctgcctggtg gaactcgctg ggcaaactca
     1261 cctctctggt tagacacgag gtcccacttg gctttcttgc cggtaacgct gtggatcgtg
     1321 atgggaacgc cagccagcgg atggtcgtcc ggcccccaaa cggttccggc aatcagggcg
     1381 cagtctttag atacggtgtt cttctgcgga tcgggagcag gcgaagggag aggtgctgcc
     1441 atcagcgagc cggcggctgc cagcagtagc aagacgaccg cgctgcgagt ggctttaccg
     1501 ccgcaatgaa acagccagag gggcacgcta ctcatcttcc tcttcgtccg tgacttcctc
     1561 tgcgtcgtcg atcacttcgt cctcgacgcg gcttagctgc aggggctcaa tcgcaaccac
     1621 ttcgaaatcc gtgccgcact cggggcagga gacgacttcg ccctcgtcca cctcgtcctc
     1681 ttcgaagtcc agatcgctct cgcattcagg acaaattacc atggccgact tcctcccatt
     1741 tgcgctcggc aaatgcgctg aagagaatca cagcgcatcc acccttttat atttagaatc
     1801 tcttgcacct agcgtcaagt aatagacaca tgagatgcgc ttaagttttg cgaggtttcg
     1861 atgcccaaaa tgaaaatgtt tcttgcaggc gccataggtt tgtggttgac attgtcggcg
     1921 gcaacggttc cggcgtgcgg ccaggttgac aacattccag tgcctccgcc cgacccgctg
     1981 attgtggaag ccctgaaggc ggtgtcggcc cagcgtattc aacagaccat cgagaagctc
     2041 gtgagcttcg agacgcggca caccttgtcg tcggatgttc cggcttccag cggcaaaggc
     2101 attaatgctg cggcggagtg gatcaggagc gagttcgagg cctactccaa ggcttgtggc
     2161 ggctgcctcg aagttaagac ggacgaattc acccaggatc ctgcatcgag gattccgaag
     2221 cccaccaagc tgaccaatgt gtatgcgatc ttgcgcggca gcgatccggc aaatgcctcg
     2281 cggatgttcc tggtcacggg gcactatgac tcccgcaata cggaaactaa cgacgccacc
     2341 ggcgcctcgc caggggccaa cgacgacggc agtggcacag cggtgagcct ggagtgcgct
     2401 cgcgtgctga gccagcatcg cttcccggcg acgatcgtgt ttgtcgccgt cgcaggagaa
     2461 gagcaggggc tgtacggcag cgcgcacatc gcgaagatgg ccaaggcgca gggctgggac
     2521 ctcgaggctg tgctgaacaa cgacatcgtc ggaggcgacc ggactcccgg aagcaccacg
     2581 caagatcggc gcaccgttcg tgttttctcc gaaggcattc cggcgaccgc cgccgaggct
     2641 gagttgaagc tgattcgcgc gttgggaggc gaaaacgacg gggcttcgcg ccaactcgcc
     2701 cgctgcatcg ctggtgtttc gaaggcgtat cttcccgcca aggaatttca cccgctggtg
     2761 gtgttccgcc gcgaccgcta tttacgaggc ggcgatcata tttcgtttaa cgagcaggga
     2821 ttcgcggctg tccggttcac cgagtatcgc gagaattatg accaccagca ccagaacgtc
     2881 cgcaccgaga acggcatcga atatggtgac ctcccgaagt tcgtggacta cgactacatt
     2941 gccaatgtgg cgcgggtgaa cgctgcgacg ctggcatcgc tggcctcggc cccagcgcct
     3001 cccgcaaaag tgcgggtgtt gacgaaggcc ctgcaaaacg attccactct agtatgggag
     3061 gcatctcccg gtggactcgc cactggatat ctcgtgttgt ggcgagatac aggcgctccc
     3121 gactgggagc acgcgcaggc ggtcgctaac gtcacgcagg ccaccgtgcc gatgtcgaaa
     3181 gacaatgtgg tcttcgcagt gcaggccatg gatgccaagg gacatcgcag tcttcccgta
     3241 acccctgcgc cggagcgcta agcgctgcgg aggaaagtcc gaacggagtt ttccagatgc
     3301 gacgcggtac cacgatgtcc ctcagctttc caccgttcac tcgggcggta aaacaactcc
     3361 tgatcgccaa cggagcggtg ttcctcctgg ttgcggtgat aggtgcgttt acgtcctttg
     3421 atgcggggcg ttgggccgat cttcatttcg gactcgttcc cacgttggca gtgttccacg
     3481 gttggatctg gcagttcgtc acctacgcct ttttgcatgc gggcctgctg cacattctgt
     3541 tcaacatgct tgcgctatgg atgttcggcg cgcagctcga ggtggactgg ggctacagcc
     3601 tgttcatgca gttctatttc ttctgcgtca tcggagccgc aatcacgact gccgtggttt
     3661 cgtttaccgg gctcttgggc gcatctccgc tggttccaac cgtgggcgcg tccggcggga
     3721 tttacggcat cctggtcgcg ttcggcatct tgcacggcga ctcggagatc atgctgttcc
     3781 cgatcccgtt catgatcaag gcccgctact ttgtgatcgg actgattctt cttgcgatgt
     3841 atggagccct atcgtccgcc cacagcatgg gccaatcgac tgcctacatg gcacacctgg
     3901 gtggaggact gtttggctac ctcttcctga agttcgtccc acgacgggga atcggttttc
     3961 tcagctccga gcggctgtac gggctgcgga attcctacta tcgctggaag cggaaacgtg
     4021 cagcgcgcaa gttcgaggtg tacatgcgga agcatgatga ccgcggcgac tatcggcacg
     4081 acgactattt cgacgagaac ggaaacatgc gcgatcccgc cgcgcgcgat agccaaaaga
     4141 aaaacggtga cgcacgtcca ccgtgggtga actgaaacgc cgtaagctag ctctcgaaca
     4201 tcgtttccag gttctcgtac accgtgggga tgctgcacag actgggaagc tgctgccagt
     4261 aacggtgcac gtcggggtcg atctgggcct cgccgcgaag ttcttccgac ttccagcggc
     4321 gcaggtgaat gaagcggtca gggcgatcgc tatcgcgagt gaggaaatcg ctggaatatc
     4381 cctttgactt catcatggca taaaactcac gcagcattgt gagcagttct tcctctttgc
     4441 cgggcagtcc ttccagaatt gccaccgaca ccacttcgct cgccatgtta tgggcctctc
     4501 aaactagtac aagattaaaa agactccggg gccagctccc ggcagtctga ttcggtatct
     4561 ctgtgtcatc cctgacttgg atcaaacgca taaagttggt cgcgccggtc gtgttttgcg
     4621 tacccgcgac caggccaaaa acgtctccgg gcgacaaaac gttgagacgc cttaattcct
     4681 cttccgcaaa ctcggccatg cgctcgaccg acagcttcgg aggacacatc agcggggtgg
     4741 tgccccagag cgcgttcata cgattgcgca cggtctccga agacgagaac gcgtagatgt
     4801 ccgccgcagg acgatacttc gataacatcc gcgccgtggt cgcggtttcc gtgaacaccg
     4861 ctatggcacg catcttcagg tccttggccg cgtgtgccat ggactcgcaa atcgtctcgg
     4921 caaccgacag atgacgggcc tcgcgatggc ccggcgtcag cgggttcaac gagtgggcct
     4981 cggtttccgc cacgatgcgt gccatcattt ccaccgcttc gcgcggatac ttgccgcttg
     5041 ccgtctcgcc cgacaacatc acggcgtcgg tgccatcaaa caccgcattg gcgacgtcgc
     5101 tagcttcggc acgagtcggt cgcggactat cggtcatcga ctccagcatc tgcgtcgcgg
     5161 tgatcacggg tttgcggaag tcgagcgcgc ggcgaatcac gaacttctgg atgagcggga
     5221 ctttctccgg cgcgacttcg acgccgagat ctccgcgagc caccataacg cccacagctg
     5281 cctcgagaat cccgtcgaga ttttctatgg cctggggctt ctccagcttt gcaatgagtc
     5341 caacgtcgct gcgataggcg gcgatggcat tcttcgccct gaccacatcg ttcacggtac
     5401 gtacgaacga gattgcaacc aggtcgacac cctgcttcaa tccgaattcc agatctcgca
     5461 gatctttctc ggtcagcgac ggaatgctga cgttggtgcc gggaaggttg atgccctggt
     5521 tctcgcccag catgccgcca ttcagcacct cgcacaccac ttcgctctcg cggacttcct
     5581 gcaccaccag ctcgatacgt ccgtccgaga gcagaatgcg ttcgcccggc ttcacgtcgt
     5641 cggccaggtt ctgataagta gtggagatga tttccgtcgt gcccgccaca tcgttcggcg
     5701 taatggtgac tgtctgtccc ttcttaaggg cgacaggcgc gcctgatgcc aggcggccgg
     5761 tgcggatctt ggggccctgc agatcctgca tgatgcagat gctgcgattc agctggcggg
     5821 ccaccttgcg cagacgctcg atcaccaccg catgttgttc atgcgtgccg tgggagaagt
     5881 tcaatctggc aacgtccatc cccaacagca tgagatcgcg aatcatctcg tcggagttgc
     5941 tggccggacc gatggtacag acaatcttcg cgcggcgccg cgaggtgttt ggactccgaa
     6001 agggatcgtc gctaggctcg aaaccacctg attttgtgct ctctatttcc gccatagttc
     6061 acagtgccgc gacgcgcttc tgcgccgaat taaaatccga atgtgctcca aacaaaaagc
     6121 gcggatagcg cagagcgttg aattctgctc cgatcaacac caccatcgac accaggtaca
     6181 accagatcaa aagcgccatg gcggtcccga ccgagccgta gatcacgccg taattggcgt
     6241 atcgccgcag gtaccagccg aataacaccg ttacagaaac ccacaacacg gtcgccacta
     6301 ccgacccggg caacaccctg tgccaaggct gtgttctggg aacgccatga tgataaacca
     6361 gtccgatcac cgcaatgctg gtcatcgtgg cgatgatcca gcgcaccgcc gtccatagcc
     6421 ccagaatgta gggcgtcaac tcgtggccgg catgaaatat ggtccagcgc tcgatctggt
     6481 tgccgaaaac cacgaggaac gacgcgaatc ccatggggaa tcccgccatc accaccaatt
     6541 ggaaagcgat gagacgttct ttcaccaggc cccagatttt tggcatctgg taggccttac
     6601 gaaagccttc catccaagag atcattacgc cagaagcggt ccacaaggta agaacgctga
     6661 tccagacaag aaactgggtc tgcaccggct tggcgcccgt gacgtactct gtgaccgcgt
     6721 tgttggtacc ttcgggaaga acacgtccca ccgcgcgaaa gatctcacgc acgaacactc
     6781 cactgctttg cgaacgcgag agtattgagg ccagcaccag gatggcgggg aagaatgtca
     6841 aaatggcgga gaacgccgcg cccttggcca cgccgaaagc gtcgtgctcg aaggcacgcc
     6901 agaacgcaag ccgcagatga ttgaagaagc gtgacatagt agccagaaca cggagcaggg
     6961 cgcgcctaaa gtgtacacac ccttacaaaa caggggcgaa ttgattcgcc ccatgaaacc
     7021 gcttgctgag aagccctccc gagaaaaacg ccgcccgtgg aacacacgag cggcgctgat
     7081 tcgcaaacaa attactcggc tgtgagttct tctgcctcgt tttcgccttc gtgacggagc
     7141 agttccagcg cgcgttcggc ttcctcatac gcggccgtga cctcctcctg cactttgact
     7201 gcggcctgtt ccatctcctc ggagagccgg acattgcggt agtactccat gccggttccc
     7261 gcgggaatga ggcggcccac gatgacattc tccttgaggc cgcgaaggtg atcgacggcg
     7321 ccctgaattg aggcctccgt caacacgcgc gtggtctcct ggaaggaggc cgccgagatg
     7381 aaggagtcgg tcgagagcga ggccttggtg atgcccagca gcagcggacg gcccgtggcc
     7441 ggcttgccgc cgttggcaat cgtcttctcg ttctcttcac ggaagcggaa cttgtcgatc
     7501 tgctgttcca gcaggaacga cgtgtcgcct acgtcttcca ccttcaccca gcgcatcatc
     7561 tggcggacga tcacttcaat gtgcttgtcg ctgatgttca cgccctgcag ccggtagact
     7621 tcctggattt cgttcaccag gtaagcctgc agctcctttt cgcccagcac ggcaagaatg
     7681 tcgtgcgggt tgagcggtcc atccatcagc ggctcgccag ccttaatgcg ctcgccttcc
     7741 tgcacgttga tgtgaacgcc acgcggcacc tggtattcct tctcggtacc gttgtcggcc
     7801 accacgtaga tcttgcgctg gcctttggcg acgtcgccga acttcacgat gccatcgatt
     7861 tcgctgatga cagcggtttc gcgcggcttg cgggcttcga acagttcgac cacgcgcggc
     7921 agaccgcccg tgatgtcctt ggtcttggtg gtttcgcgcg ggatcttcgc cagcacttcg
     7981 cccggcgtga cctcgtcgcc atccaggatc atcaagtgag cacgcgaggg catcaggtaa
     8041 cgcttgctgc ccttggcgcc cttgatgacg atcgcaggct ggcgcttctc gtccggcgaa
     8101 tccatcacga cgtgacgtga caggccggtc acttcgtcga cttcctcgtg tagcgtcagg
     8161 ccttcctgca ggtccttgaa ctgcacggtt ccgccaattt ccgtgaggat cgcgaaagtg
     8221 tacggatccc actcgaccag cacgtcgccg agtgaaacct gctggccgtc ttcgaccttc
     8281 accttgccgc catacacgac tgcgtagcgc tctttttcgc ggcccttctc gtccaccacc
     8341 gcgatcgaac cattgcggtt catcgcgacc aggtagcctt ccttcgaacg gaccgtgatc
     8401 aggttcacga agcgcaccgc gccattgttc ttggcatcga ggcgcgactg ctcgctgacg
     8461 cgagacgccg taccgccgat gtggaacgta cgcatggtga gctgggttcc gggctcgccg
     8521 atggactgcg ctgcgatgac tcccgtggcc tcgcccagtt cgaccaggcg tccggatgcc
     8581 aggttgcgtc cgtagcacat aacgcaaacg ccacgcttcg actcgca
//
LOCUS       DASRKK010000699         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_657582, whole genome shotgun sequence.
ACCESSION   DASRKK010000699 DASRKK010000000
VERSION     DASRKK010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657582"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..996
                     /gene="nuoB"
                     /locus_tag="VFC15_17015"
     CDS             187..996
                     /gene="nuoB"
                     /locus_tag="VFC15_17015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZM11911.1"
                     /translation="MLRRSLRGGVQVIPVEQLIGNCSGSRMPDVDPAALTQTLAEKAV
                     LACPTSALSGASPVAERHLTLDYGECIGCGRCIEVAAPAFLEAKNFAYCGVRRDGLVR
                     RWDISANTELVAAAASPERVKRQIHSMLGRALNIRQLDAGSCNGCEAEITALTNPHYD
                     LERFGIHFVASPKHADMLLVTGPVTRNMANAVRATFEAVPAPKLVVAVGACGCSGGIF
                     AGASAVAGPVDALLPVDAYIPGCPPTPAMLVTGILKALRQADGLRSSSKNL"
     gene            complement(1040..1351)
                     /locus_tag="VFC15_17020"
     CDS             complement(1040..1351)
                     /locus_tag="VFC15_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11912.1"
                     /translation="MAQPNPQQVIGDRQPQPLLLVPALDDEKLMAEGKDLCLKCNSFT
                     ERITQDGEQGNQDRHHRQEAYRCPALSTIGTARIEFLVGTARLDLAVPPPNSHSRLMP
                     K"
     gene            complement(1362..2294)
                     /locus_tag="VFC15_17025"
     CDS             complement(1362..2294)
                     /locus_tag="VFC15_17025"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZM11913.1"
                     /translation="MPDSPERYIVVMIRNIGLSMLLITGASGRVARRAAALLAQRGYA
                     LRLMTRTPQRAPKLEGTEVVRGDFAEPATLDDAFTGVSTALVVSGSGRPGERARLHRN
                     AFEAAARGRVRHLVYLSLQGASPTSKYPFSRDHSLSEQYLAATGIPYTVLRNAFYLDM
                     FLDKFDAYGVIRGPANKSRGAFVSREDAARTAAAVLADPPGGTHDVTGPEALSLADVA
                     GHFSAMTGRQLRYQPESAQSARDRLSEREPDAWRVDLSVGWFQAIAARELERTSDAVF
                     RFTGKVPLNLEEYFSLFPELLQPLRLTQDQDSGS"
BASE COUNT          577 a          817 c          840 g          617 t
ORIGIN      
        1 cggggagctc ctgtactgga tcgaaactcg cgatgcgcga atcgcacgtt gtcacatccg
       61 atcgccgtcg ttccaaaact ggcctgcatt accccaggcg gtcattggca acatcattgc
      121 cgattttcca ttgattaata agagcttcaa tctgtcctat tcgggatgtg accgatgatc
      181 tccaatatgc tgcgccgcag cctcaggggt ggagtgcagg ttattcctgt cgaacaatta
      241 atcgggaatt gcagcggtag tcgcatgccg gacgtcgacc ccgcggcgct gacgcagacc
      301 ctggcggaga aagctgtctt ggcctgcccg acatctgcac taagcggtgc gtcacccgtt
      361 gctgagcggc atctcacgct cgattatggc gagtgcatcg gatgcggtcg ctgcatcgag
      421 gttgccgcgc ctgcttttct tgaagcgaag aacttcgcgt actgtggcgt ccgccgagac
      481 ggtttggtcc gccggtggga tatatcggca aatacagaac tcgttgcggc ggctgcttct
      541 cccgagcgtg tcaaacgtca aatacattcg atgttgggac gcgcgctgaa catccgtcaa
      601 ttggatgctg gttcctgtaa tggttgtgag gcggaaatca ctgccttgac aaatccgcac
      661 tacgatttgg agcggtttgg cattcatttc gtcgcttctc ccaagcatgc ggatatgctt
      721 ctcgtcaccg ggccggttac caggaacatg gcgaacgccg tgcgcgccac atttgaggcg
      781 gtacctgcgc caaagctggt cgtcgcagtg ggtgcgtgtg ggtgcagtgg aggaatcttt
      841 gccggcgctt cggccgtagc tggccctgta gatgcacttc tgccggtgga tgcctatatt
      901 cccggatgcc caccgacgcc tgcgatgctc gtgacaggca ttctcaaggc cttacgacaa
      961 gccgacggcc ttcgatcaag ttcgaagaat ttatagagtt ggtcgaatcg ggtgcagacc
     1021 ccgaacagac gatccgccct tacttgggca tcagccgtga gtgactgttg ggtggtggaa
     1081 ctgcaaggtc aaggcgggct gtccctacca agaactcgat tctcgctgta ccgattgtac
     1141 ttaacgcggg acatcgatag gcttcttggc gatgatgccg gtcctggttc ccttgctcgc
     1201 catcctgcgt gattcgttcc gtaaacgagt tgcacttcag gcagagatct ttgccttccg
     1261 ccatcaactt ctcgtcgtcc agcgcaggaa ccagtaacag cggctgcggt tgtcggtcgc
     1321 cgatcacctg ctgtggattg ggctgtgcca tattccaacc gtcagcttcc ggaatcctga
     1381 tcctgtgtta gccggagcgg ttgcaacagt tcggggaaca agctgaaata ctcctccagg
     1441 ttcagcggca cctttcctgt gaaacggaaa accgcgtcgc tggtgcgttc caactccctg
     1501 gccgcaatcg cctgaaacca cccgacggag agatctacgc gccaagcgtc tggttctcgc
     1561 tcgctcaacc gatctcgtgc ggattgtgcg gactctggct ggtagcgtag ctgccggccg
     1621 gtcatggcag agaaatgacc ggccacatcg gcaaggctca gcgcctctgg tccggtaaca
     1681 tcgtgcgtac cgccgggcgg atcagccagc accgcagccg cggtccgggc tgcatcctcg
     1741 cgggaaacga aggcgcctcg cgacttgttc gccggaccgc ggatcactcc ataggcatcg
     1801 aacttgtcca gaaacatgtc caaatagaag gcgtttcgaa gtacggtata cgggataccg
     1861 gttgccgcaa ggtactgctc gctcagagaa tgatcgcgag agaaggggta cttcgaagtc
     1921 ggagaggcac cctggagcga caaatagacg aggtgccgta cccgcccccg cgcggcggct
     1981 tcgaacgcat tccggtgcaa tcgcgcacgt tcccctggcc tgccagaccc cgagaccact
     2041 agcgcggtgg acacgcctgt aaacgcgtca tccagcgttg ccggctctgc gaagtcgccc
     2101 cgaacaacct ccgttccttc cagtttcggc gctcgttgtg gagtgcgggt catcaggcgc
     2161 aatgcatatc ctctttgcgc cagcaatgca gcagcacgtc gtgcgacacg tccggaagcg
     2221 cccgtaatca ggagcatgct cagcccgatg ttgcgaatca ttacgacgat ataccgttcg
     2281 ggggaatccg gcatacgttg aatcattctg ggcgcagtgt gctcttattt cagaagaagc
     2341 acacatcttc acgggtcgca atggtctgaa cctggaaaaa actgccgggc gcaattcttc
     2401 aacagtgcag gcaatagttc gctcaaaaca ggcggtcggg agtcgacaaa cggtatcgcg
     2461 ggcctgtagt cggcgctcgg aactattcgt gccgtgcccg tggcgtgggg cagaggtgca
     2521 tcttgcggta gctctgcctc ggcctccttt gaccgatgaa taatgagagg gccccagggt
     2581 cgttcagtgt ttggggaaga tcgggctggt aggcagccga attcgcctcg gggtagttgg
     2641 cgacggatgc ccaatcataa ggatgagacg ccacaacgct cagcggccgg atgtcttttt
     2701 ctgtctcgac acaggaacag aaaagcccgc caggcggcgg gcgttcaagt tgcgaatccg
     2761 cgtgaattac ggaacagtaa tcacaacttt ccccacctgc gcattcgact cgagatattt
     2821 gtaggcgtcc accgtctgcg caaagggaaa a
//
LOCUS       DASRKK010000700         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_461813, whole genome shotgun sequence.
ACCESSION   DASRKK010000700 DASRKK010000000
VERSION     DASRKK010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461813"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFC15_17030"
     CDS             <1..827
                     /locus_tag="VFC15_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11914.1"
                     /translation="PDAKNPPLFTTAEVTKTSNPDGPGISATSKFIEKYRQLNSAFVY
                     IDACEGETLTDAFTKVVTGSEGARVAIGAKGCPGVEGSFKYAQRFFADLDGQPVERRS
                     DDTRLELRAVGPAMTTENKVDKSVQFSISLFGSATTLAPRVREERGGFAFKCPMGKGD
                     TIDLVFDTECEKDKLPDIEVTGITIGDAKWQGDIKFISHELKIPVTGPEAPARTFKFT
                     MKGDKLQSGNNQATLDGNTSPALVKDGKGVNAQGPSNSKASPDSYVVEYQDCKRVH"
     gene            complement(1263..2186)
                     /locus_tag="VFC15_17035"
     CDS             complement(1263..2186)
                     /locus_tag="VFC15_17035"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZM11915.1"
                     /translation="MQAQGGLTIERMCQLGQVSRAGFYRHWQRHEPGAEEMEVRARIQ
                     EIVLQHRGNYGYRRVSWELQHQGMVVNHKRVLRLMQQDHLLAVRRRKYILTTEARHDL
                     PVYVNLAARLELSGIDQLWVADLTFIRLRDEFLFLAVVLDAFSRRVVGWAVDDSLHAA
                     LTIAALRKAIAERRPAPGLVHHSDRGIQYAAQDYLRLLREHGIVPSMSRTGNPYDNAK
                     CESFIRTLKREEIYTRDYRDRADLEAHIAEFLQQYYNRQRLHSALGYRSPEEFENSLR
                     AAPVLPAAQMSFFRHEEIYRSDVGFDSGRQA"
     gene            complement(2186..2521)
                     /locus_tag="VFC15_17040"
     CDS             complement(2186..2521)
                     /locus_tag="VFC15_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11916.1"
                     /translation="MTHRSWTVEQKRAAVERMKSCGHDKLAAKLKVQRRQLYAWRAQL
                     KRLDAGGSVAAENKKDQQREQEKQRLQAALASKVLEVEFFKGALRRIEARRQSSSGCG
                     ETAFTNRCE"
     gene            complement(2757..>3966)
                     /locus_tag="VFC15_17045"
     CDS             complement(2757..>3966)
                     /locus_tag="VFC15_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11917.1"
                     /translation="ATLVSLLIAFIVLLLVWKGIGIYKIAGTHYGPLSAFFLDKETNS
                     YSLSKFQLVAWTAVAVFGYVYLLCCQTLIQWNFNFPSIPSGWPTLLGVSAGATVAAVG
                     ITSTRGSKGAGPLSPSMADFISSGGLVLSDRFQFFVWTLVGCGGFLFLILRNDPSKLT
                     ELPDVPSGFLYLMGISAAGYLGGKLVRLPGPVVKELLVASVVPGVPGTTSAVLTISLK
                     GENFSKNASVKVDEDELRTDQFQIECVKPQDQPADPSFCSEIKVTLKEADKYLEGQHK
                     LALTNKDGQMAVSSFPADPLTIDPVNDLLEGTTPVDVVVNGANFAVGLTAVWKNAALK
                     ETTIGAAQVQKKTDTQLIVTLAPGPAGKATLTLISPINLRAHTDVNVKKMTTDPVSSV
                     AAGADPTTSQ"
BASE COUNT          810 a         1176 c         1155 g          825 t
ORIGIN      
        1 gtcccgacgc taagaacccc ccgctattca ctaccgccga ggtcacaaaa accagtaatc
       61 cggacggacc cgggatttcc gccacgtcta agttcataga gaagtaccgc cagctcaact
      121 ctgcctttgt gtatatcgat gcctgcgagg gcgagacttt gaccgatgcc ttcaccaagg
      181 ttgtcactgg cagtgaggga gccagggtag cgataggcgc taagggctgc cccggggtgg
      241 aaggttcctt caagtacgca cagcgttttt ttgccgacct cgacggtcag ccagtcgaga
      301 ggcggtcgga cgacacacgc ctggagttgc gagcggtggg ccctgcgatg actacggaaa
      361 acaaggtgga taagtccgtg cagttctcca tctcgttgtt cggcagcgcg acgacattgg
      421 cgccgcgcgt tcgcgaggag cgcggaggct ttgctttcaa gtgtcccatg ggaaagggtg
      481 acaccatcga cctcgtcttc gacacggagt gtgaaaaaga caaacttcct gacatcgagg
      541 tgactggcat caccattggc gatgctaaat ggcaaggaga catcaagttc atctctcatg
      601 aactgaagat tcccgtcacc ggtccggagg ctccggccag gaccttcaag ttcacgatga
      661 agggcgacaa actgcaaagc ggcaacaacc aggccacact cgatggcaac accagccctg
      721 cgctggtgaa ggatggcaag ggagtcaacg cgcaaggccc ttccaatagc aaggcctctc
      781 ccgacagcta tgtcgtggag tatcaggatt gcaagcgagt gcactagcaa gcctgaggcc
      841 ggtgggcttt gagcggttcc aggattggtg tatccgctcg tacgggtagt gcgggccttc
      901 aggcccgcgt aagatccgca caattcctac gcggttttct ttgtgcaact gtacgcgggc
      961 ctaaagagcg tgtgtgagaa cttgttttga gttggctcgg tggaacgatg gcgccaaatc
     1021 cagcgccgta ggcgcggctc agtttagccc agcacggagc cgcaggcgga gtgaagtgaa
     1081 ccccttgcgt gagacacggg gcaaagtccc aggttgccgt tcgctgagtt ttgctcgacg
     1141 gacggaggtt gctggttgca gcatgaggct ggctgaggaa gcgcaggcga gctctgctgg
     1201 agagcaactc gccgaggaat actcggccgg aaactcatcg cgacgatggg ggctccggga
     1261 gatcaagctt gcctcccgga gtcaaagccc acatcggagc gatagatttc ctcatgccta
     1321 aaaaaactca tctgggctgc ggggagcaca ggcgccgccc gcaagctatt ctcaaactct
     1381 tccggggagc ggtagccgag cgccgaatgc agccgctggc gattgtagta ctgctggaga
     1441 aactcggcga tatgcgcttc caggtcggcg cggtcgcgat agtcccgggt gtagatttcc
     1501 tcccgcttca gggtgcggat gaagctttcg catttggcgt tgtcgtaagg attgccggtg
     1561 cggctcatgc tgggcacgat accgtgctcc cgcagcagcc gcaagtagtc ctgcgccgca
     1621 tactgaatgc cgcgatcgga atggtgcacc aaaccaggcg cgggccggcg ctcggcgatg
     1681 gctttgcgca gcgcggcgat ggtcaaggcg gcatgcaacg agtcgtccac tgcccagccc
     1741 accacccggc gggagaaggc gtccagcacc accgctaaga acagaaactc gtcccgcagg
     1801 cggataaatg tcaggtcggc cacccagagt tgatcaatac cgctcagctc caggcgcgcc
     1861 gccaggttca catacacggg caagtcatgc cgcgcttcgg tggtaagaat gtacttgcgc
     1921 cgccgcaccg ccagcagatg gtcctgctgc atcaggcgca gtacccgctt gtggttcacc
     1981 accatcccct ggtgctgcaa ctcccaactc acccgccgat agccatagtt gccgcggtgc
     2041 tgcagcacaa tttcctggat ccgggcgcgc acctccatct cctcggctcc gggttcgtgc
     2101 cgctgccagt gacgataaaa accggcccgg ctcacctgcc ccagttggca cattcgctcg
     2161 atggtaaggc caccttgcgc ctgcattact cgcacctgtt cgtaaatgca gtctcgccac
     2221 agccgctgct gctctggcgt cgagcctcga ttctgcgcaa ggcacccttg aaaaattcca
     2281 cttccagcac tttgctggcc agcgcggcct gcaaacgctg cttttcctgc tcccgctgct
     2341 gatccttctt gttttcggca gcgacactgc cgcccgcgtc caagcgcttc agctgcgctc
     2401 gccaggcgta taactgccgc cgctgaacct tcagttttgc cgccaacttg tcgtgcccgc
     2461 agctcttcat gcgctccacc gcggcacgtt tttgttccac tgtccaactt cgatgagtca
     2521 tcccattctc cattcgctgt aatatgggac tctccgccgt gtctcagttt aggggttcac
     2581 ttcagagtgc tgggtaaagt gggaagtagg gccgagtccc ttcaagggac ggcacagttc
     2641 tcacgcacgc tgtgaaggcc cgcactactt tcgttgaacc ttgtccgcag caatggttga
     2701 tatgccagag gcgcatttcc actcccccat tgtcccgtcc gtgaagttct tcccctttac
     2761 tgtgacgtcg taggatcggc gcctgcagcc acactgctga cgggatcggt cgtcatcttc
     2821 ttgacgttca cgtcggtgtg cgccctcaaa ttgatcgggc tgatgagcgt aagcgtggct
     2881 ttgccggcag gaccaggcgc aagggtaacg atcagttgcg tgtcggtttt cttctggacc
     2941 tgggccgccc ctattgtcgt ttccttgaga gccgcgttct tccacaccgc agtcaagcca
     3001 actgcaaagt tagcgccatt gaccacgacg tccacaggcg tcgtgccttc caagagatca
     3061 ttgactggat cgatggttag cggatcggcg gggaagctcg ataccgccat ctgcccgtct
     3121 ttgttggtca gggctagctt gtgttgacct tcgaggtatt tgtccgcttc ctttagcgtc
     3181 actttgattt ccgagcagaa ggaaggatct gcgggctggt cctggggctt cacgcattcg
     3241 atctggaact ggtccgtcct cagttcgtct tcgtcgacct tgacggaagc attcttggag
     3301 aagttctcac ctttcaggct gatggtgagc actgcggacg tagttcccgg gacgcctgga
     3361 accacagacg cgaccagaag ctctttgaca accgggccgg gaagacgtac cagtttcccg
     3421 cccaggtatc cagcggcgct gatgcccatc aagtagagga agccactagg cacatcgggc
     3481 agttctgtca acttcgaagg atcgtttctc agtataagga acaggaaacc gccgcatcct
     3541 accagtgtcc aaacgaagaa ctggaaacga tcgcttagga ccaggccgcc gctgctgatg
     3601 aaatcggcca tcgaaggcga gagcggcccc gcacccttgg aaccgcgggt ggacgtgatt
     3661 ccaacggcag cgaccgttgc cccggcgctc acgcccagca gcgtcggcca accgctgggt
     3721 atgctcggga agttgaaatt ccattgaatc agcgtctggc agcagaggag gtacacgtag
     3781 ccaaatacgg cgaccgcagt ccacgccaca agttgaaact tcgacagact gtacgaattg
     3841 gtttctttgt cgaggaagaa ggcgctcaat gggccgtagt gcgtacctgc gatcttgtag
     3901 atcccgatac ccttccacac tagcaacaac acgatgaatg ctattaagag agagaccagg
     3961 gtggcg
//
LOCUS       DASRKK010000701         5127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_265186, whole genome shotgun sequence.
ACCESSION   DASRKK010000701 DASRKK010000000
VERSION     DASRKK010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265186"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFC15_17050"
     CDS             <1..471
                     /locus_tag="VFC15_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11918.1"
                     /translation="GVPPEEADACLNVRLVLRPTIPHPDLSYLIDNTHGAAGGVRMQL
                     GRDGGRPVDFLDVDGRVTREQSTKLEEAIWQHLPDLRPLRADQFGDYNDRNEVRHSDP
                     LAITQLLLTYHLLRVHSGDKPMTFASPVAALSRLESKTLKPDPHMAPVGSVTEP"
     gene            558..2633
                     /gene="tkt"
                     /locus_tag="VFC15_17055"
     CDS             558..2633
                     /gene="tkt"
                     /locus_tag="VFC15_17055"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZM11919.1"
                     /translation="MDISSQDKKQTTGMLPKPHRETPEQQLETLCINTIRTLSMDAVQ
                     KANSGHPGTPMALAPLAFTLWDRYLRHNPRNPHWPGRDRFILSNGHACMLLYSMLYLT
                     GYDLSLDDIKQFRQWGSKTPGHPEYGLVPGVEATTGPLGQGVGNSVGMAIAQRWLASQ
                     FNKDGHDLFDYRIYAFCGDGDLMEGVSNEAASVAGHLGLSNLIWFYDDNHITIEGSTS
                     LAFSDDVAMRFAGWHWNVLRVSDVNDLDLLDVAINAAQRETQHPSLIIVDSHIGWGAP
                     HRQDTKEAHGEALGEEEVRLTKINYGWPPDAHFLVPEEVKAYMGKAVERGAQWEQEWN
                     DRYNAWTKDFPDLAETCQQMLNRELPAGWDKDIPSFSTDAKGVATRESSGKVLNAVAK
                     NVPWLMGGAADLYPSTKTLIDGAGKFEKGSYSGRNFHFGIREHAMGSIVNGMSLSGLR
                     PYGATFFIFSDYMRPVLRLAAMMDQPVIFIYTHDSFCLGEDGPTHQPIEHLISLRAIP
                     RLLLFRPADANEVAAAWRYIMPLKKAPAALVLTRQAVPTFDRTKYAPAEGLAKGAYIM
                     ADSGGTPDIILIGTGSEVQLCVGAYERLTAEGIKARVVSMPCWRLFDEQSADYKRQVL
                     PPEVRARVAVEAGTSLGWSEYVGPDGYVVARNDFSASAPVKELLKHFGFTVDDVVAKA
                     REIIKRIKP"
     gene            2735..2953
                     /locus_tag="VFC15_17060"
     CDS             2735..2953
                     /locus_tag="VFC15_17060"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZM11920.1"
                     /translation="MPGFVTIPISSRQGVEHDNTNVICMGSRVIGPELALDLGRIFLG
                     AQFTGVSRHVRRLAKIESLEREKLRGLK"
     gene            2950..4086
                     /gene="tal"
                     /locus_tag="VFC15_17065"
     CDS             2950..4086
                     /gene="tal"
                     /locus_tag="VFC15_17065"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZM11921.1"
                     /translation="MSNNPLLQLHSFGQSLWLDQMRRSLLTTGELKRLIDNDGLRGLT
                     SNPTIFEKAISGSQDYAEQLGQLARAGSNIPQIYEEIVVQDIGGAADTFRGVYDANNG
                     GDGFVSLEVSPLLANDTKATIEEAQKLFARLNRPNVMIKVPGTPAGLPAIEELIASGL
                     NINVTLIFAVEVYEKVADAYIKGLERRAAAGQPVDRIASVASFFVSRIDSMVDKQLGQ
                     LAEKATDEQQKQRINGLMGKAAVANAKLAYASFKHIFGGERFATLKAKGARVQRPLWA
                     STSTKNPAYPDTMYVETLIGTDTVNTVPPQTLDAFRDHGKVAPTLEAGLDEAHHTFDE
                     LKAVGVDMKTVTDKLTEDGVKSFSDSFRELIEVIEARATKSCAA"
     gene            4368..4607
                     /locus_tag="VFC15_17070"
     CDS             4368..4607
                     /locus_tag="VFC15_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11922.1"
                     /translation="MRHQLLILQRRNQKQRLRLSVADRLLWVWLSRIWADWRLSHRLV
                     KPKTVIAWHRRGFRLFRPTPVFVRWMKAQHGNKLI"
BASE COUNT         1074 a         1528 c         1512 g         1013 t
ORIGIN      
        1 ggtgtgcccc ccgaggaggc cgatgcgtgc ctgaacgtca ggctcgtctt gcgtcccacg
       61 attccccatc cggatctgag ctacctgatc gacaacactc acggcgcggc gggcggcgtc
      121 cgcatgcaac tgggccgcga cggcggccgc ccagtagact ttctcgatgt ggatggccgc
      181 gttacgcggg aacaatcgac aaagctggaa gaggcgatct ggcaacactt gcccgacctg
      241 cgtccgctgc gcgctgacca gttcggcgac tataacgatc gcaacgaggt gcggcacagc
      301 gatccgctgg ccattaccca actgctgctg acctatcacc tgctgcgcgt ccacagcggt
      361 gacaagccca tgacctttgc atccccggtg gcggcgctca gccgattaga gtcaaagacg
      421 ctgaagccgg acccgcacat ggcgccggtc gggagtgtca cggaaccgtg agagccagga
      481 ggaccgccgt taaatggctg gtacctcctg ccggattgac ttaaacagca gaaaggtggg
      541 gtcgagtgag tagtcaagtg gacatttcct ctcaggacaa gaaacagacg accggaatgt
      601 tgcccaaacc ccatagagag acgccggagc agcaactcga gactctttgc atcaacacca
      661 tccgtacgct ttcgatggac gcggtgcaaa aggcgaacag tgggcatccg ggcacgccca
      721 tggcgctggc gccgctcgca tttacgttgt gggaccgcta tttgcgccat aaccctcgca
      781 atcctcattg gccaggccgc gaccgcttta ttctctccaa cgggcacgct tgcatgctgc
      841 tgtattccat gttgtacctg accggctacg atctttcgct cgatgatatc aagcagttcc
      901 ggcagtgggg atcgaagact ccggggcatc ccgagtatgg cctcgtgccg ggagtggaag
      961 ccacgaccgg ccccctgggt cagggcgtcg ggaactcggt aggcatggcc attgcgcagc
     1021 gctggctggc ctcgcagttc aataaagacg gccacgatct tttcgactac cgcatctacg
     1081 cgttctgcgg ggacggcgac ttgatggaag gcgtcagcaa cgaagctgcc tcggttgccg
     1141 gccatcttgg gctgtctaac ctgatctggt tctacgacga caaccacatc accatcgagg
     1201 gtagcacttc gctcgctttc agcgatgatg tggccatgcg cttcgccggc tggcattgga
     1261 acgtgctacg ggtgagcgac gtgaacgatc tcgatctgct tgatgtggcg atcaacgccg
     1321 cgcaaaggga aacccaacac ccgtcactca tcatcgtgga tagccacatc ggctggggcg
     1381 ccccgcatcg gcaggacacc aaggaagctc acggcgaagc gctgggcgag gaggaagtcc
     1441 gccttaccaa gatcaactac ggctggccgc ccgatgcgca cttcctggtt cccgaggaag
     1501 tgaaggccta catgggcaag gccgtcgagc gtggcgcaca gtgggagcag gaatggaacg
     1561 atcgctacaa cgcgtggact aaagactttc cagacctcgc ggagacatgc cagcagatgc
     1621 tcaaccggga gttgcccgcc ggatgggaca aggacattcc gtccttctcg accgatgcga
     1681 agggcgtcgc cacgcgcgaa tcgagcggca aggtgctgaa cgcagtcgcg aagaacgttc
     1741 cgtggctgat gggcggcgcg gccgaccttt atccctcgac caaaacgttg atcgacggcg
     1801 ccggcaaatt cgagaagggc agctacagcg gccgcaactt ccacttcggt atccgcgagc
     1861 atgccatggg ctccatcgtg aacggcatgt cgctctcagg actgcgtccg tacggcgcga
     1921 ccttcttcat cttttcggat tacatgcgac cggtattgcg actggcagcg atgatggacc
     1981 agccggtgat cttcatctac acacacgatt cgttctgcct gggagaggac gggccaacgc
     2041 accagccgat cgagcatctc atatccctgc gagccattcc gcggcttttg ctcttccggc
     2101 cagcggacgc gaatgaggtc gccgcggcgt ggcgttacat tatgcctctg aaaaaggcac
     2161 cagcggcgct ggtgctcacc cggcaggccg ttcccacgtt tgaccgcacg aagtacgcgc
     2221 cggctgaggg tcttgccaag ggtgcgtaca tcatggctga ttccggcggc acacccgaca
     2281 tcatcctgat cgggaccggc tcggaggtgc aactttgcgt tggcgcctat gagcggctca
     2341 cggcagaagg cattaaggcg cgcgtcgtct ccatgccttg ctggagactg ttcgacgagc
     2401 aatccgcgga ttacaagcgc caagtactgc cgccggaggt gcgggcgcgc gtggccgtcg
     2461 aagcgggcac cagcctcggc tggagtgaat acgtcgggcc tgacggatat gttgttgccc
     2521 ggaacgactt cagcgcatct gctcctgtta aggaactgtt gaagcacttt ggcttcaccg
     2581 tggacgacgt ggtggccaag gcgcgcgaga tcataaagag aatcaaacct taatcctgga
     2641 gccataatgt tgccatgaaa agagccgtga gcggtgctca tctgcggcag tggagtaggc
     2701 gcttcggttg cggccaacaa gctccctgtc attcgtgccg ggctttgtca cgattcctat
     2761 ttcgtcacgt cagggcgtag agcacgacaa cacgaatgtg atatgcatgg gctcgcgcgt
     2821 catcgggccg gaattggcgc tggacctggg gcgcatcttc cttggggcgc agttcacagg
     2881 agtgtcgcgc cacgtccggc ggctcgcgaa gattgaatcg ctcgaacgcg agaaattgcg
     2941 aggactgaaa tgagcaacaa tcccttacta cagcttcatt cattcggcca gagcctctgg
     3001 ctcgaccaga tgcgccgttc cttgttgact acgggcgagc ttaagcggct tatcgataac
     3061 gacggtctcc gcggcctcac atcgaatccg acaatcttcg agaaggccat cagcggcagc
     3121 caggattacg ccgagcagtt ggggcaactt gcccgtgccg gaagcaacat cccacagatt
     3181 tacgaggaaa tcgtcgttca ggacattggc ggcgccgccg acactttccg cggcgtttac
     3241 gacgccaaca atggcggcga tggatttgtc agcctcgaag tttctccgtt gctggccaac
     3301 gacaccaagg cgaccatcga agaggcccag aaactgttcg cgcgcctgaa ccgcccgaac
     3361 gtgatgatca aagttccggg caccccggcc ggacttcctg ccatcgagga gctcatcgcc
     3421 tccggactga acatcaacgt caccctgatc ttcgcggtcg aggtgtacga aaaggttgcc
     3481 gatgcctaca ttaaaggctt ggagcgtcgg gccgccgccg gccagccggt tgaccgcatt
     3541 gcttcggtcg ccagcttttt cgtcagccgc attgactcca tggtggacaa gcagctcggc
     3601 cagctagcag agaaagctac cgacgagcag cagaagcaac gcatcaacgg gctgatgggc
     3661 aaagctgccg ttgccaatgc aaagctggct tatgcgtcgt tcaagcacat cttcggcggc
     3721 gagcggtttg caaccctgaa ggcgaagggc gcgagggttc agcgacccct gtgggcctcc
     3781 accagcacca agaatcctgc ctatcccgac accatgtacg tggaaaccct catcggaacc
     3841 gatacggtca acactgttcc tccgcaaacg ctcgacgcct ttcgcgatca tgggaaggtc
     3901 gcgcccacgc tcgaggctgg tctcgacgaa gcgcatcaca ccttcgacga actgaaggcc
     3961 gtcggcgttg acatgaagac cgtcacagac aagctgaccg aagacggcgt caaatcgttc
     4021 tcggattcgt tcagagagct gatcgaggtg attgaggccc gcgcgacgaa gtcatgtgcg
     4081 gcatgatcga tcgccacacc gctgcaccta gccactacga agacgacttt caagacacgc
     4141 tgaaggttcc gacacagaga acttcgttac cccgcctgtg gaagaaggac cccacagtgt
     4201 ggaagagcga ccctatccct acggaaaatt cgatcgtcgg tgtaccgctt gcacttagcg
     4261 cggtacaccg atcggcttct tggcgatgat gacggtcctg gtttccttgc tcgctgttct
     4321 gcgtgattcg tttccgtacg cgaattgcac cacaagaaat ccttgccttg cgtcatcaac
     4381 ttctcatcct ccagcgcagg aaccagaaac agcgcctgcg cctgtcggtt gctgatcgtc
     4441 ttctctgggt gtggctctcg cgtatctggg cggactggag actgtcgcat cgccttgtga
     4501 aacctaagac ggtgatcgca tggcatcgca gaggctttcg gctcttccgc cctacgcctg
     4561 tgttcgtccg atggatgaaa gcccagcacg gaaacaagct catctaagac attggctcag
     4621 ggtggggacg ggtgagcaat gtgttggcca aagctggaat gcaaataacc gctctggatg
     4681 tcgaaccccg aatcgcgtct gaattcaacg taatcaaagc cgacagcacc gagtacgttt
     4741 tcgacaaaga tcctgtagtc atgctctgtc ggccttgcca tgacggtgag tttgttcgga
     4801 agacaattct tcgcgctctc aattctggag ttcgagacgt catctatatc ggactgcagc
     4861 gaaatgcgcg agccgatctg ggggggggta gtttgtggta tttgttataa tcagctcgcg
     4921 ttagagttac ggtttcccgt tatcggctgc cccatgcaac aacccgtccg acttgcgcct
     4981 cccatccttt cctccgattt cgccaaactg ggggaggaga ttcgcgccgt ggacgccgcc
     5041 ggagccgatt acatccatgt cgatgtcatg gacggtcatt tcgtgccgaa cctgacgata
     5101 ggcccgagcg tcgtgaaagc cctgcgc
//
LOCUS       DASRKK010000702         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1146280, whole genome shotgun sequence.
ACCESSION   DASRKK010000702 DASRKK010000000
VERSION     DASRKK010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146280"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1972
                     /locus_tag="VFC15_17075"
     CDS             317..1972
                     /locus_tag="VFC15_17075"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM11923.1"
                     /translation="MYFRVKRTGSYAYLQIVESFREKGHVHQRVLSTVGRLDVLQAAG
                     QLDSLIRSGLRFCKRLAVIDAHAAGQTEPVSVQRVGPDLVFGRLWESLQLGTIIKRAL
                     QSRHFEFDVERAVYLTVVHRLFASGSDRAAERWRQAYRLPGTETLELHQLYRAMAFLG
                     EPLKDQPGMRVLDTPRCTKDWIEEELFEPRRDLFSEIDLVFFDTTSIYFEGQGGQELG
                     RHGKSKDHRPDLPQMVVGLVLDLHGWPLCCELWPGNTADVTTLMAVVNRLRQRFRVRR
                     VCIVADRGMISADTITALESEEVDCDYILGARMRSMKEVRDRVLADRGRYQEITPERQ
                     TAKDPSPLKVKEVLIEDRRYVVCLNEEQRRKDAADRKAIVEHLRQQLKQGDKELIGNK
                     GYRKYLQATSGKRFTIDEAKIQKEARYDGKWVLRTNLADEPEIIALAYKELWMVEDMF
                     RTMKSILETRPIYHKRDETIRGHVFCSFLALLLKRGLEQRLKEKGETWEWAEILRGLD
                     NLQEVEALFQGKRFILRSQVLGQAHKAFMAAGVALPPTLREKV"
BASE COUNT          483 a          564 c          692 g          474 t
ORIGIN      
        1 gcccctctat taaccgatcc ctttacgccg tgcccttttc atgccctttg ctgctaccgc
       61 cggcggttcg gggctcttcg cacagaaacg ggctgctgta gggatgcaat agatagagac
      121 tcgccgttac ccctctgact catcttctac agtgaatcgg ggtttgggat cacaaatctt
      181 aaaagaacct gccaattgtg tgtgggcaat tttcctaagc tcgaaatgta gtgccaaatt
      241 ctgatggcac ccattaagtt gttattgcgc cccgatggcg atagcgcgat ggatgtagag
      301 gaaaatatct ggcgctatgt attttcgggt caaacgcacc ggttcctacg cctaccttca
      361 gatcgtggag agttttcgtg agaagggcca cgtgcatcag cgcgtattga gcacggtggg
      421 gcggttggac gttctccaag ccgccggtca gttggattcg ctgatacgct cgggcctgcg
      481 gttttgtaag cgactggccg ttatcgatgc gcacgccgcc ggccagaccg aaccggtcag
      541 cgtgcagcga gtcggtccgg acctggtctt cggccgtttg tgggaaagcc tgcagttggg
      601 aacgattatc aagcgcgcgc tgcagtctcg ccacttcgag ttcgatgttg agcgggccgt
      661 ctatttgacc gtggtgcatc gtttgtttgc ctcaggcagt gatcgggcgg ccgagcggtg
      721 gcgccaggcg tatcggttgc cggggaccga aaccttggag ttgcaccaac tctaccgcgc
      781 gatggctttc ctgggcgaac cgctcaaaga ccagcctggg atgcgggttc tggacacgcc
      841 gcgctgcacc aaggattgga tcgaggaaga actctttgag ccgcggcggg atttattcag
      901 cgagattgat ctggtcttct tcgacaccac ttccatctac ttcgaaggcc agggcggcca
      961 agagcttggc cggcatggta agagcaaaga ccatcggccg gatctgccgc agatggtggt
     1021 gggattggtg ttggatttgc acgggtggcc gttgtgttgt gaactatggc cgggcaacac
     1081 ggccgatgtc accacgctca tggcagtcgt caaccggcta cgccagcggt tccgggtgcg
     1141 ccgggtttgt atcgtcgcgg accgggggat gatcagcgcg gacacgatta cggctttgga
     1201 atcggaggag gtcgattgcg actacatatt gggtgcgcgg atgcgctcga tgaaggaggt
     1261 gcgtgaccgg gtgctggcgg atcggggccg gtatcaggag atcacgccgg aacggcaaac
     1321 ggccaaggac ccctccccct tgaaagtcaa ggaggtgctg atcgaagacc ggcgctacgt
     1381 ggtctgcctc aatgaagaac aacgccgcaa ggacgcggcc gaccgcaaag ccatcgtgga
     1441 acatttgcgc caacagttga aacaagggga caaggagttg atcggcaaca agggttatcg
     1501 gaaatatctg caggcaactt cggggaagcg ctttaccatt gacgaggcga agatccagaa
     1561 ggaagcccgc tacgacggca aatgggtgtt gcgaaccaac ttggctgacg aacccgagat
     1621 catcgccctg gcgtataaag agctgtggat ggtggaggac atgttccgga cgatgaagtc
     1681 gatcttggaa acgcgcccga tctatcacaa acgggacgag acgatccgag gccatgtatt
     1741 ctgtagtttt ctggcgctgt tgctcaagcg cggactggag cagcgcctga aggaaaaggg
     1801 ggaaacctgg gagtgggcgg aaatcctgcg tggtttggac aatctgcaag aagtggaggc
     1861 tctttttcag ggaaagcgct ttattttgcg cagtcaggtg cttggccagg cgcacaaggc
     1921 gttcatggcg gcgggggtgg ccttgccccc aacgttaagg gagaaggtat gaaccaaaac
     1981 tcaaggagag tgaaaatgta gtgccaagac catttactgt cttcgtaagt ccttgataat
     2041 caatgacaat gctcttcgca ctgtagaaga tgagccaaag gggccaagtc cgtgcttcct
     2101 catctggctc agagccagga ccaacgcaaa atcgcaacag cccccgaacc atgcgcccag
     2161 ctcgaattcc aatctacggt ttgacggttt gaccaggttg tcagggtaac ttc
//
LOCUS       DASRKK010000703         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_168488, whole genome shotgun sequence.
ACCESSION   DASRKK010000703 DASRKK010000000
VERSION     DASRKK010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168488"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFC15_17080"
     CDS             <1..967
                     /locus_tag="VFC15_17080"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZM11924.1"
                     /translation="YRLHPLVPDLIEYRDISKDPNQILQKVAVIDTFEGKATDAMRSR
                     GLANWGLSLGRQRLGLLALHNHPQFMQNIHLPRLDTPTGQIDIAALDMIRDREHGVPR
                     FNEFRRQYGLRQLTSYDDFMDKSLPADSAAHKQQQGMVAQLREVYGTHVCDSSKVITD
                     AQVNEDSSPINDCLGHPNGFVVDNIEDVDTVVGWLAESTRPHGFAISETQFVVFILNA
                     SRRLYSDRFFTSSFRPEFYTQMGIDWVNHNGPGPEMIEAGMPNGHKQPVSPLKRVLLR
                     NIPELTPELQHVTNAFDPWGRARGEYYTLDWKPRPDAIADPAFKQ"
     gene            1799..>3368
                     /locus_tag="VFC15_17085"
     CDS             1799..>3368
                     /locus_tag="VFC15_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZM11925.1"
                     /translation="MVEGIQSPAQVTYWLITGRTLFVILNLLGLMCFFYIVSKRMVPL
                     FRGERDWRFDRPLVRLERVLQFWLGQWKHPRYRTAGIMHILFFAGFLILATQAFSLLI
                     LGVAGNFVLPGSSGEIGHIYDIVKDYAATVVFLCMIVAAVRRVVFKPARYAVPARYGK
                     GHPVDAIFLLALIALLMASESLFEASKAAFQTQQGQPVEFLAALSLPWMLKNVLISTP
                     LPTLQTLHLGAYFVDVLTFYFLLCYRPFGIQFHVETSLFNVYFAKLDRGTVKPVRWGV
                     SDEHLDQVKSFGVKTFEDFTWKHMLDFYSCADCGRCSDQCPANAVGRPLSPRFITIKA
                     RDYSFQHYPMFGSSGNGTPLIGSIYSEDEIWSCTTCGACEEECPLLIQYIDKIVDLRR
                     GMIDDGNVPLSLQKPLKALESRGNPYGKMEKKRAEWTKAKEFSQTCTVKTLDGKSNAD
                     TLYFVDSITSYDDRMQSIGRATARILDSAGVNFGILGAAEKDSGHDARRFGEETLFMA
                     LRDHNVDAIKASGVR"
BASE COUNT          711 a          961 c          945 g          751 t
ORIGIN      
        1 gtaccgcctc cacccgctgg ttcccgacct gatcgagtac cgcgatatca gcaaggaccc
       61 gaaccagatc ctccagaaag tcgccgtaat cgatacgttc gagggcaaag ccacggacgc
      121 gatgcgatcg cgtgggctgg caaactgggg tctgagtttg ggacggcagc gtctgggact
      181 gctcgcgctg cacaaccatc cgcagtttat gcagaatatt catctgccgc ggctcgacac
      241 tcccaccggg cagatcgata ttgcggcgct cgacatgatt cgcgaccgcg agcatggcgt
      301 gccgcgtttc aatgagttcc ggcggcagta tggcctgcgt caactgacca gctacgacga
      361 cttcatggat aagagtctcc ccgccgattc cgctgcccac aagcagcagc aagggatggt
      421 cgcgcaattg cgcgaggtct atggcacgca cgtttgcgat tcctcgaagg taatcacgga
      481 cgcgcaggtg aacgaagact cctcgcctat caacgactgt ctgggccatc ccaacgggtt
      541 cgtggtcgac aacatcgaag atgtggacac ggttgtcggc tggctggcgg aatcgacgag
      601 gccgcacgga tttgcgattt cggagacgca gtttgtcgtg ttcattctga atgcgtcgcg
      661 gaggctgtac agcgaccgtt tcttcacatc gagcttcagg ccggagtttt atacccagat
      721 gggcatcgac tgggtgaacc ataacggtcc ggggccggaa atgatcgaag cgggcatgcc
      781 gaacgggcat aagcagccag tttcgccgct caagcgggta ctgctgcgaa acattccgga
      841 actgactccg gagttgcagc acgtgaccaa cgcgttcgat ccttggggac gcgcgcgtgg
      901 cgaatactac acgctcgatt ggaagccacg cccggacgcg atcgccgatc ccgcgtttaa
      961 gcagtaacgc gaacgtagtc gcattgaggg ccgatggcgt agcatggagt ggtagtcaca
     1021 cattcacagc cgaagaagta cccgcagaat tcgctcccag ccaatgaaaa ttacagacaa
     1081 cacaaaggtc tttctagcgt ttctcgcggt gatgttcgcg ctccaagctg ttttcgatgg
     1141 tcgcagcatc ttgaacgcgg tcactagcga tgcgcttgaa acatttctgc aaagactggc
     1201 tattgccgcg cttattgcgg ctggtgcgac tttcctttgg aatctagtcc gccgcaaacc
     1261 gtagtttttc tgggtttgtc gaagacataa ttccgacaac tttgagattg ctggcggcca
     1321 aaggggtccc ctcgactcct cgctgcgctc gtcgctcggg atgacaccca ataaactact
     1381 tgcgcgctca tgaataatac agctgaagcg ccgcactacc cttagaaacc ccatacatct
     1441 attgtggaac cgctagatcc tgccctgcga cgacccatct gcatcgcaaa gtcgcaaccc
     1501 actccagttt ctgctcgtat ccgggcaacg tcacgtcaca aagtacccgg catcacaggc
     1561 acaccgtact ccgcgtcaca gacatatgtc cggttcccct tcaaactgaa gcgtactccc
     1621 gggtgctcag gtcagtgcac tagcgcctga gctgctcctc agcgttacga gggcgaaagc
     1681 aattgctgcc atttgttgtc ctcttcctgc gcgtccggca gccgcgtcgt ccattggccc
     1741 tctgaacaac atttacagga gtaacgggaa cctggcccag taggagatgt tgagcattat
     1801 ggtggaaggc atccagtcac ccgcgcaagt gacgtactgg ttgataaccg ggcggaccct
     1861 attcgtgatc cttaatcttc tggggctcat gtgctttttc tacattgtgt caaagcgcat
     1921 ggtaccgctc tttcggggcg aacgcgattg gcgctttgat cggcccttag tccgattgga
     1981 gagggtcctc caattttggc tggggcagtg gaagcatccg cgctaccgca ccgccgggat
     2041 catgcacatc cttttctttg cgggcttcct tatcctggcg acccaggcct tctcactgct
     2101 gattctcggt gtcgccggga atttcgttct gccgggctcc tcaggggaaa tcggtcatat
     2161 ctacgacatc gtcaaggact acgctgcaac cgttgtcttc ctttgcatga ttgtggccgc
     2221 cgttcgccga gtggtcttca agcccgcgcg ctacgcggta cccgccaggt acgggaaagg
     2281 gcacccggtc gacgctatct tcctattagc gttaattgcg ctgctgatgg cgtccgagag
     2341 cctcttcgag gccagtaagg ccgcctttca gacacagcag ggccagcccg ttgaattcct
     2401 cgctgccctg tcgttgccgt ggatgttgaa gaatgttctt atttcgactc cgttgccgac
     2461 actccagact ctccacctcg gcgcctattt tgtggatgtg ctgaccttct acttcttgct
     2521 gtgctatcgg ccgttcggaa tacagttcca cgtggaaaca tctttattca acgtctactt
     2581 cgcaaagttg gaccgcggaa ccgttaagcc agtgcgatgg ggcgtgtccg acgagcatct
     2641 ggaccaggtg aaatctttcg gcgtgaagac cttcgaggac ttcacgtgga agcacatgct
     2701 cgatttctat tcctgcgccg actgtgggcg gtgttcggac caatgcccgg ccaatgcggt
     2761 gggaaggccg ctgtcgccaa ggtttatcac tatcaaggca agagactaca gttttcagca
     2821 ttatccgatg ttcggcagtt caggcaacgg cacgccattg atcggcagca tttactcgga
     2881 agatgaaatc tggtcttgca cgacttgcgg cgcttgcgaa gaggaatgcc cgttgctgat
     2941 ccagtacatc gacaagatcg tcgatttgcg acggggcatg atcgacgacg ggaatgtacc
     3001 cctgtccctg cagaagccgc tgaaggccct cgagagccgt ggcaatccat acggcaagat
     3061 ggagaagaaa agggctgagt ggacaaaggc caaagagttc tcccagactt gcaccgtcaa
     3121 gaccctggac gggaagagca atgcggacac cttgtacttc gtcgacagca tcacctccta
     3181 cgatgaccgc atgcaatcga ttggacgcgc tacggcgagg attctcgatt cggccggagt
     3241 gaatttcgga atcctgggcg ctgcggaaaa agatagtgga catgatgccc gtcggtttgg
     3301 cgaagaaact ttgttcatgg cgctgcggga ccacaacgtc gatgccatca aggcgtcggg
     3361 agtccggc
//
LOCUS       DASRKK010000704         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_72408, whole genome shotgun sequence.
ACCESSION   DASRKK010000704 DASRKK010000000
VERSION     DASRKK010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72408"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFC15_17090"
     CDS             <1..730
                     /locus_tag="VFC15_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZM11926.1"
                     /translation="TGGSILAAERALQCGIAFNIGGGFHHAFPDHGEGFCMINDFAVA
                     IRAMQKRGKIRRAMTIDCDVHQGNGTAFIFGNSTHKPSNLPSLPAPTLSSVANGTVSH
                     GPCKDVFTISLHQENNYPYSKPASSIDVNLPDGCGDSEYLAWLDNALSSGLRQFDPEL
                     ICYVAGADPFSEDQLGGLTLTIEGLKRRDELIFRAARARDIPVMTVYAGGYANHVEDT
                     VTIHCNTVMAAAEVYRAAATEMTS"
     gene            complement(727..1797)
                     /locus_tag="VFC15_17095"
     CDS             complement(727..1797)
                     /locus_tag="VFC15_17095"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM11927.1"
                     /translation="MAQYGNVLVLSTIERGELTRWAQSRSLPAGEVFRARLVLALADG
                     FSYRQIEKRLHTSSPTIARWAQRFQQHRLDGLNPRHKGSRPRVVTAAVQARILRRTLQ
                     PPPDGSTHWSCRKLALTLGVSKSSVQRVWSQAQLKPHRLDRYMASNDPEFESKATDII
                     GLYLNPPQHAAVFCVDEKTAIQALDRLDPVLPLSPGRAERHGFEYYRHGTLSLYAALN
                     VKSGKVTGKTAQRHTSADFIDFLSGVVRQTKWAREIHIVLDNLSAHKTKAVEQFLQHN
                     PRVRFHFTPTYSSWLNQVELWFAKIQRDVIDRGVFTSVADLARKLRRYIRAYEKSARP
                     FRWTYTNPQRRIRTNTIAGTAH"
     gene            complement(1889..2011)
                     /locus_tag="VFC15_17100"
     CDS             complement(1889..2011)
                     /locus_tag="VFC15_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11928.1"
                     /translation="MKNGKSNIPAFADTLSDQQITSVVAHVRTFGKPGKRPPAK"
BASE COUNT          469 a          657 c          706 g          476 t
ORIGIN      
        1 caccggggga tcgatcctgg cggccgagcg tgccttgcag tgcgggatcg cattcaacat
       61 tggcggcgga tttcatcatg ccttccctga ccatggcgag gggttctgca tgatcaacga
      121 ctttgcagtg gccatccgtg cgatgcagaa gcggggaaaa atccgccgcg ccatgaccat
      181 cgactgcgat gtacaccagg gcaatgggac ggcgttcatc ttcggcaact ccacgcataa
      241 gccgagcaac ctgccctcac taccggctcc gacgttaagc tccgtggcga atgggacggt
      301 cagtcacggg ccttgcaaag atgtgttcac catctcgctg caccaggaga acaattaccc
      361 ctacagcaag ccagcttcat ccattgacgt caatttgccc gatgggtgcg gcgactcgga
      421 gtacctggcg tggctggaca acgcgcttag ttcagggctg cgacagttcg atccggaact
      481 gatctgctat gtggcgggtg ccgatccgtt cagcgaagat cagttgggcg gcctgacact
      541 gaccattgaa ggcttgaagc ggcgggatga attgatcttc cgggcggcac gggcgcgcga
      601 cattccagtg atgacggtgt acgccggggg atacgccaat catgtcgagg acacagtgac
      661 catccactgc aacaccgtga tggcggctgc ggaagtttac cgggcggctg ctactgagat
      721 gaccagctag tgggctgtcc cggcgattgt gttagtacgg atacgccgtt ggggatttgt
      781 gtaggtccaa cggaaagggc gagctgattt ctcgtaggcg cggatgtagc gccgtagctt
      841 gcgagccaag tcggccaccg aggtaaagac gccccggtcg ataacgtcgc gctgaatctt
      901 agcgaaccag agttccacct ggttgagcca ggacgagtag gtcggggtga agtggaagcg
      961 cacgcgcggg ttgtgctgca agaactgttc tacggctttg gttttatgag ccgaaaggtt
     1021 atccagtacg atatggatct ccctggccca tttggtttgc cgcacgacac cgcttaagaa
     1081 atcgatgaag tcggcgctag tgtgccgttg ggcagttttg cccgtcacct tacccgactt
     1141 cacattcagc gccgcataca gcgagagcgt gccgtggcgg taatactcga agccgtgtcg
     1201 ctcggcccgt ccgggcgaga gcggcaacac cgggtccaag cggtccagag cttgaatagc
     1261 ggtcttctca tcaacgcaga atacggctgc gtgttggggc ggatttaagt agaggccgat
     1321 aatatcggtc gccttgctct cgaattctgg atcgttactg gccatataac gatccagacg
     1381 atgcggtttc agctgggcct ggctccagac acgctgcacg ctcgacttac tgacacccaa
     1441 cgtcaaggcc agcttgcgac agctccaatg cgtgctccca tctggcggag gctgcagcgt
     1501 gcggcgcagg atgcgagcct gaacagcggc tgtgaccacg cgggggcgac tgcccttgtg
     1561 acgcgggttc agaccgtcca ggcggtgctg ttggaaacgc tgggcccagc gagcgatggt
     1621 cggagaactc gtatgtagtc gcttctcaat ctggcgatag ctgaaaccgt cagccagtgc
     1681 cagaaccaag cgggcgcgga agacctcgcc tgcgggcaaa ctacgcgatt gcgcccaacg
     1741 cgtcaactcg ccgcgttcga tagtggaaag aacgagcacg ttaccatact gtgccatgct
     1801 cattcgacgt aagacaaccg cttacgttat cgctgggacg aaccactagg cgtcaccggt
     1861 taaatcgcaa ggcgttactt ggcatcgtct actttgcggg tggccgtttt cccggctttc
     1921 caaaggtacg aacatgggcc accacgctgg taatctgctg gtcggacagt gtgtcagcaa
     1981 atgccgggat attactctta ccgttcttca caatctgctc cagtcagcgg tggactagcc
     2041 ctgcactgcc ggcgaacgta agtcagccgc gttaagactc cggcctgccg atgacgcgcc
     2101 tcccgccggc gtgggcgggc ctggagtgcc gccctttgtt gccgccttct ttcatgcgct
     2161 cttcgcggca actggcaaac gcacgcgcga tctgccgttg tccagaagtg acgcgggttg
     2221 tcgctcatat ttcgtcccgc cctagccaaa ggaaggctag aacttggcac ccggacagat
     2281 tgacgctcca cgaatgcaat gtgggaat
//
LOCUS       DASRKK010000705         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_462691, whole genome shotgun sequence.
ACCESSION   DASRKK010000705 DASRKK010000000
VERSION     DASRKK010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462691"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..620
                     /locus_tag="VFC15_17105"
     CDS             348..620
                     /locus_tag="VFC15_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11929.1"
                     /translation="MKKASTERRNDDLRPEYDLARLAGGARGKYYRQAVAGTNLILIE
                     PELARVFRDSESVNRALRLLVETATASAAPARPRHRAPKKRLQPAR"
     gene            complement(1690..>3073)
                     /locus_tag="VFC15_17110"
     CDS             complement(1690..>3073)
                     /locus_tag="VFC15_17110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM11930.1"
                     /translation="AAIPGLETLIDADYIVRGEGISWMRRYLGEDPEAAIVHPEIVSG
                     FGFRILGMRVPDNKCDTAATIIPSVGCPLGCNFCTTSSFFGGKGKSIHFYETGDQLFE
                     VMQQMETSLGVSSFFMMDENFLLNRPRAMQLLARMKEAGKSWALYVFSSVNAVRKYTM
                     EELVQLGVSWIWVGLESPNSSYAKLKNSDTRALAAELRRHGIKILGSTIVGLEHHTPE
                     NIHEEIEYAVSHGTDFHQFMLYTPVPGTPLFAQMQQEGRMLDDVDLADIHGQFKFNYK
                     HAAISRDESKEFLDWAFRHDFERNGPSLFRICETTFQGWKRYHNDPDLRVRRRFAAEA
                     RKLRTTYNAALWAMEKRLRTTNPPISQRIRELRREIERAFGTFTRMVRVVVGPILLWT
                     SKREDKRLANGVTYEPETFVERRNWIADKLRHTADVVTMHPRDAERPLTNIAVARVAV
                     DSLSQVASGD"
BASE COUNT          656 a          865 c          870 g          682 t
ORIGIN      
        1 ggccacccgt cattcggccg attcaaaatg aatgtccatc gatagggcac tccgattcct
       61 cgtgcgtagg ttgagctaaa cttaggcggt ggtctacgag tggaatgcgg cgaaggcaaa
      121 agccaacgct tacaagcacg gtgtatcctt cgaagaggca tcgacggtct tcctcgaccc
      181 tttagcctgg acgttttccg accctgatca ctctgctgct gaagcgcggg agatcacaat
      241 cgggtattca gtccggcatc gggtcatctt tgtagcgcat agccagcgcg gggaccgtac
      301 gcgaatcatc agtgcccgaa aggcgacaag gaaggagcgc aagcagtatg aagaaggcat
      361 cgacagagag gcgaaatgac gatttgcgtc cggagtacga tctcgcccgg ctcgcgggag
      421 gggcccgcgg caagtactac cgccaagccg ttgcagggac gaacctaatt ctgatcgagc
      481 cggagctcgc gcgcgtgttt cgcgattcgg aatcagtaaa ccgcgccttg cggctgctgg
      541 tggaaaccgc aaccgcgagt gctgctcctg ctcgtccgcg gcatcgcgca cccaagaagc
      601 gcttgcaacc ggcgcggtag aaacccgcac cgcaatcggc ggaaaaaaac tccacccaag
      661 caagttcggc ttgagtgggg caccctcatg ttcgtaggct gggccacccg cctacttggg
      721 tgtaagaatt agtgctcgtt atgtccttgt tcctcatcct cccccgaaat ttctgactcg
      781 tatgttatgg ataattctcg aacacttaac ggacttcgct gtaagcggtt ttcgacaagg
      841 ctgaaatttt cttctgcctc gcggagtcgg accatttgtg taaattgcgt tgaattttgc
      901 tcgtaagcgg ggagatctat attcgcagaa cgttgaagat cgcgtaacag cctgtctcgg
      961 gtcaattcga ggatctctcg gtttagtgcc agttacgaat tttctccgct gtttgtgtga
     1021 gcgcccaaag tgtcgctgct gcagcaggaa tggctgtaac aagcgcgatc agttcaagag
     1081 gagatccttg gctgagtaga tctaacttta gcttgtcgtc ctctccgatt cttttttgat
     1141 ttcgaaatcc agaatatcta gaaaatcgat acgaagaata ctcgggatca acgattacgc
     1201 gggaaaactc ataaaggaga ttgaaatcgt acaagaatga cgatacctgg tcgatgtcgg
     1261 gataatcgtc atcttcggcg agcagatgaa tccgatagta gccagcgtaa ttaggtggca
     1321 gagacattca gatggcgggt ggcccaccct ctctgatcta gaactatcac catcttccat
     1381 cctggggtgc cccacccttc tcgcctgccg ttggcgagag ggtgggaatc gtcggtcgaa
     1441 tcttcacccg tggagttatt cccatccgct cgcgtttgcg tcctgtccgt tcggactcaa
     1501 tctagactac accgtgtaat cccatccgcc tgctggcagc gcttgagtcc gcaggggcac
     1561 ggaagcagtc tatcggaacc atgacgcggc gatggcgatc acgcgactgc gcccaccctc
     1621 tcgccaaagg cgggcgagaa ggatggggta cccgattttc aacggtaacc ggcctggaat
     1681 ctagcccaat tagtccccgc tcgctacctg gctaaggcta tcgaccgcaa cccgtgccac
     1741 tgcgatgttt gtcaatggcc gctcggcatc gcgcggatgc atggtcacaa catccgccgt
     1801 gtgccgtagt ttgtcggcta tccagttgcg gcgctccacg aacgtttccg gctcataggt
     1861 gacgccgttg gccagccgct tgtcttcgcg tttcgaggtc cataacaata tcggcccaac
     1921 cacgacgcgt accatgcgtg tgaacgttcc gaaggcgcgc tcgatttcgc gccgcagttc
     1981 acgaatccgc tgcgatatcg gcggattggt cgttctcagc cgcttctcca tggcccaaag
     2041 cgccgcattg taagtcgtgc gcaacttcct ggcctccgca gcgaagcgac gccgcactcg
     2101 caggtcggga tcgttgtgat agcgcttcca tccttggaag gtagtctcgc aaatgcgaaa
     2161 caagctgggg ccgttgcgct cgaagtcgtg ccggaaagcc caatcgagaa attccttcga
     2221 ctcgtcgcgc gagatagcgg cgtgtttgta gttgaacttg aactgcccgt gaatgtcagc
     2281 caggtccacg tcgtcgagca tgcggccctc ttgctgcatt tgcgcgaaca gcggcgtgcc
     2341 cggcaccggc gtgtacagca tgaactggtg gaagtcggtg ccgtgcgaga ccgcgtattc
     2401 gatctcctca tgaatgttct cgggcgtgtg gtgctcgagg ccgacaatcg ttgaacccag
     2461 tatcttgata ccgtgccggc gtagctccgc cgcgagcgcc cgggtatcgg aattcttcag
     2521 tttggcgtag ctggagttcg gcgattcgag tcccacccag atccacgaaa cgcccagttg
     2581 caccagttct tccatggtgt acttgcggac ggcattcacc gacgagaaaa catagagcgc
     2641 ccagctcttg ccggcttcct tcatgcgcgc caggagttgc atggcgcggg gacgattcaa
     2701 caggaagttt tcgtccatca tgaagaaaga actcacgccc agtgaagtct ccatctgttg
     2761 catgacttcg aagagctgat ctccggtttc gtagaagtga atgctcttgc ctttgccgcc
     2821 gaagaaggaa gaagtagtgc agaagttgca ccccagcggg catccgaccg acggaatgat
     2881 ggtggctgcg gtatcgcatt tattgtccgg cacccgcatc cccaggatgc gaaagccaaa
     2941 gccagagacg atttccgggt ggacaatcgc ggcttcggga tcttcgccca gatagcgccg
     3001 catccaggag atgccctcgc cgcgaacgat gtaatcggca tcaattaacg tctccagtcc
     3061 ggggatggca gcg
//
LOCUS       DASRKK010000706         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_560242, whole genome shotgun sequence.
ACCESSION   DASRKK010000706 DASRKK010000000
VERSION     DASRKK010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560242"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFC15_17115"
     CDS             complement(<1..186)
                     /locus_tag="VFC15_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11931.1"
                     /translation="MSLKAVAHISQVRGGSQARIMLADDGKKFVTKLMGNPQCTRVLA
                     NDYLACRMARTVGLSVPE"
     gene            complement(309..1334)
                     /locus_tag="VFC15_17120"
     CDS             complement(309..1334)
                     /locus_tag="VFC15_17120"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HZM11932.1"
                     /translation="MPTEDKTTSPSGLEVNKLLPELEVPFSPDQVRWRVTNTTNDKKR
                     GQIVPYADSRAYADRLNALFTPHGWTREYKVETMTNITRVRKGESILSGKVLVTCTVT
                     ILGIGSHSGTGEEWADDDNGMTSADAQAFKRACSCFGLGRYFYDIQAIWVDLDQNRQP
                     VRTPTLFAWALPENWRKGMRPSGRNGNGSAAAPSNGTNGRSQANGSNSPRVSGNGHTH
                     AGDSKASASGGNGDDLDARILCLEKTVGTVLYRNMLREYGRVNQPKLIRDPAVKRTVL
                     QMLESAARGIERLDAVTKRFDSKTVEALLAKLQAPPLGEIADMKTLQHVVFGLEELAG
                     SDQHHAA"
     gene            complement(1725..3236)
                     /locus_tag="VFC15_17125"
     CDS             complement(1725..3236)
                     /locus_tag="VFC15_17125"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM11933.1"
                     /translation="MKSHRHFFTFLLLSVLLIGAASSGTAQQYKMTTPIAPGVATPDT
                     LDTSIGTLHLADGNPDTDTVRKIYDNLDQSRALQAYLLAIPIVNQAGMRDSMRKFGPD
                     NQTDVIWETLVDPKTVELTANDNTVYSFIWLDTHKGPLVVEVPPKVLGAIDDFYYRWV
                     VDIGITGPDKGQGGKYLVLPPGFTGQVPEGYIVVRPPTYGNWMPFRSFLVDGSPTPGV
                     ELVKKTLKIYQLSDAANPPAIKFANASGIPSNFVAPGDYSFWELLNTVIQEEPAAPND
                     PTMMGLFASIGIEKGKPFNPDARMKKILTDAANIGAVTARTLAFRMRDKDAFFYPDSS
                     WRLPYFGGYQFMVSPGVSNLDGYVFFYYFATGVTPAMEMKMVGQGSQYPWAATDSKGN
                     PFDGGKTYKLHLPPHIPVKDFWSVIVYDNQTRSMLQTDQHAPSVSSQDKGIKTNADGS
                     VDVYFGPKAPAGEEHNWIQTIPRKGWFMILRLYGPLEPWFDKTWRPGEIELQK"
     gene            3521..4291
                     /locus_tag="VFC15_17130"
     CDS             3521..4291
                     /locus_tag="VFC15_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM11934.1"
                     /translation="MQLRDNTILITGGGRGIGRGLAEAFQTEGNQVIIAGRGKKALEE
                     TVSGNPGMKAAVVDMTDGDSILGLAEQMKRDYPALNVVILNAGIMRTESVQSGGLDDA
                     EAIVATNLLGPIRLTAALLPMLLSKPRAAIMTVTSGLAFVPLAVTPTYCATKAAIHSY
                     TQSLRYQLRHTSVEVLELVPPYVQTELLGPGQLTDPNALPLKDFISEAMHILRHSPEA
                     TEICVERVKPLRRAEASGNYDTFYKTFNERAASAHETL"
     gene            complement(4437..>4798)
                     /gene="ssb"
                     /locus_tag="VFC15_17135"
     CDS             complement(4437..>4798)
                     /gene="ssb"
                     /locus_tag="VFC15_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZM11935.1"
                     /translation="PEVKYSQQGTAIAQFSVATTERWKDKGGELQEHTEWFAVKAFGR
                     RAEVVGEHLRKGTRIYVEGRKRTESWDDKQTGAKHFRDLVYVDRIEFLDSKGNGHAEA
                     TSGTYDETAVTDQDVPF"
BASE COUNT          947 a         1307 c         1481 g         1063 t
ORIGIN      
        1 ttctggcacg ctgagcccga ccgtgcgcgc catccgacag gccaagtagt cattggccag
       61 cactctggtg cattgtgggt tgcccatgag cttggtcacg aatttcttgc cgtcatcggc
      121 cagcatgatt cgcgcttgcg agccgccgcg aacctgggag atgtgtgcga ctgccttcag
      181 agacacgttt ccgcctccgc atgtttgtgg tggagttttt tggtatggac gccatttctg
      241 gctgtgttca acgagcgaag ggcacacgta tccctgtgcc ctcgcattcg tggtggcggt
      301 ggtggtgatt aggctgcgtg gtgttggtcg gaaccggcga gttcttcgag gccgaagacc
      361 acgtgttgca aggtcttcat gtcagctatc tcgcctaatg gcggggcttg gagcttggcc
      421 agcagcgcct cgacggtttt cgagtcaaac cgtttggtaa cggcgtccaa tcgctcaatg
      481 ccgcgtgcgg cggattcgag catctgcagg acggttctct tcacggcggg atcacggatc
      541 aactttggct ggttgacgcg gccgtactcg cgaagcatgt tgcggtacaa cacggtgcca
      601 acagtctttt ccaggcacag aatgcgcgca tcgagatcgt cgccgttgcc tccgctcgcg
      661 ctggctttgc tgtctccggc gtgagtgtgg ccattgccgg aaacccttgg ggagttgctc
      721 ccgttggctt ggcttcgtcc atttgtgccg ttggaagggg cggcagcgct cccgttgcca
      781 ttcctacccg aaggccgcat tcctttgcgc cagttctccg gaagtgccca ggcaaacaac
      841 gtgggtgttc tcactggctg gcggttctgg tcgagatcga cccaaatggc ttggatatcg
      901 tagaaatacc ggccaagacc aaagcacgaa caggcccgct tgaaggcctg cgcatcggct
      961 gacgtcatgc cgttatcatc gtcggcccat tcttccccgg tgcctgaatg cgacccgatg
     1021 ccaaggatcg tgaccgtgca ggtgactaac accttgcccg agaggatgct ctcgcccttc
     1081 cttacgcggg tgatattggt catcgtttcg accttgtact cacgggtcca gccatgtggc
     1141 gtgaagagcg cgttcagccg gtcagcatag gcgcgggaat cggcgtacgg cacgatctga
     1201 ccgcgcttct tgtcgttggt tgtgttggta acccgccacc ggacttggtc aggtgaaaac
     1261 gggacttcaa gctctggaag gagcttgttt acttccagtc ccgagggaga tgtcgttttg
     1321 tcttcagttg gcatggcgag gcaccctcct tcaggtacct gaggatttgt tgaggttcag
     1381 ttgtgattta aggaaatgcg agtcggccgt tggctggagt tgggttggat accacgggcg
     1441 gctgctggcc gatttagagc ggaaggatat ggtttttgct gtggctattt atcttgatcg
     1501 ccggtgccag cccgaaccgg atttctcaat acgcacggaa tgagtcagcg ggatcgacgg
     1561 gcaaaagcgg ggggctgaag gaaaaaccgg attacctacg ggatggggat atcacaacat
     1621 ctatctgcgc agagccaacc taaactcgtc gtccagccag ggaaaccgtt gctggggatc
     1681 ataatgctgc ggggcagctc gcaagagctg cccccgaacg tgaattactt ctggagttcg
     1741 atttcgccgg gccgccacgt cttgtcgaac cacggctcaa gcggaccata cagtcgcagg
     1801 atcatgaacc agcccttcct ggggatggtc tggatccagt tgtgttcttc acccgcagga
     1861 gctttggggc cgaagtaaac atctaccgac ccgtccgcat tcgtcttgat ccccttgtcc
     1921 tgactgctga cgctgggagc atgctgatcg gtttggagca tggagcgcgt ctggttgtca
     1981 tagacgatga ccgaccaaaa gtccttgacg ggaatgtgag gcggcaagtg cagcttgtag
     2041 gtcttcccgc cgtcgaacgg attgcccttc gagtccgtcg cggcccacgg atattgcgag
     2101 ccctggccga ccatcttcat ttccatcgcg ggagttacgc ccgtagcgaa atagtagaag
     2161 aagacatagc catcaaggtt cgagacgccc ggcgagacca tgaactggta accgccaaag
     2221 tagggcagac gccacgaact atccgggtag aaaaaagcgt ctttatcgcg catgcggaag
     2281 gcaagtgttc gcgccgtcac ggcaccgatg ttggcggcgt cggtgaggat cttcttcatg
     2341 cgggcgtcgg ggttgaacgg cttgcccttc tcgattccga tggaggcaaa caggcccatc
     2401 atggtcggat cgttgggtgc ggcgggctcc tcttgaatga cggtattcag aagctcccag
     2461 aatgagtagt cacctggcgc cacgaagttc gacggaatgc cggaagcgtt ggcgaacttg
     2521 atggccggcg gattcgcagc atcggaaagc tggtagatct tcagggtttt cttgaccaac
     2581 tcgacgcccg gcgtaggaga gccatccacc aggaagctac ggaagggcat ccagtttccg
     2641 taggtgggtg ggcgcacgac gatatagccc tcggggacct gccccgtgaa tccgggcggc
     2701 agcacgagat acttgccacc ttgtcccttg tcggggccgg tgatgccgat gtcaaccacc
     2761 cagcggtaat agaagtcatc gatcgctccc aagaccttcg gggggacctc gacgaccaat
     2821 ggtcccttgt gggtgtcgag ccaaatgaag ctatagaccg tgttgtcgtt cgcggtcagt
     2881 tccaccgtct tgggatcgac gagtgtttcc cagataacgt cggtctggtt gtccggccca
     2941 aatttccgca tggaatcgcg cattccagcc tgattcacga tgggaatggc aagcaggtag
     3001 gcctgcaaag cgcgagactg gtccaggttg tcgtagattt tcctgaccgt atccgtgtcg
     3061 gggttgccat cggcgaggtg cagcgtaccg atggatgtat cgagtgtgtc gggcgtggca
     3121 acgcccggag cgatgggggt agtcatcttg tactgctggg cagtgccgga actggcagcg
     3181 cctatcaaga gtacggataa cagcagaaac gtaaaaaagt gtcggtgcga tttcatcgaa
     3241 tcctcccaga tcgatgtttc cgccgtcagc tttcacggtt tggagttaca ggagccttca
     3301 ttgcagccgc cgtgacagct catactgaac atagaggtgg cgtaaacgag ctatttcatc
     3361 acaaatcggg aaaagacgga tgtgaccgcg gtcactggtg aggtattttg agctggatca
     3421 ctggcgagaa caggccgttc cgtaggttaa cgaatcggaa gcttcgccac cccgccttga
     3481 tattatgacg cggtggcata gtggagagag ggagggcctc atgcaactga gggacaacac
     3541 tatcctgatt accggcggcg gaagggggat tggtcgcggt cttgctgagg cttttcagac
     3601 cgagggaaac caagtgatta ttgccggccg tggaaagaag gcgctggaag aaacggtgtc
     3661 cggcaatccg ggcatgaagg ctgccgtggt cgatatgacc gacggcgact cgatcctagg
     3721 cctcgccgag cagatgaagc gtgattatcc tgccctgaac gtcgtcattc ttaacgctgg
     3781 cattatgcgg acggagtcgg tgcaaagtgg tggcctagat gatgccgagg cgatcgtggc
     3841 gacgaacctg cttggcccga tccgcctgac agcggcgctg ctgcctatgt tgctgtcgaa
     3901 gccgcgggcg gcgatcatga ccgtaacttc cgggctggca tttgtgccgc tagcggtgac
     3961 tccgacctac tgcgccacca aggccgcgat ccattcctat actcaatcgc tccggtatca
     4021 actgcggcac acatcggtgg aggtcctgga actggtcccg ccgtacgtgc aaacggaact
     4081 gctgggaccg gggcagctca cggatccgaa cgcgctgccg ttgaaggact tcatttccga
     4141 agcgatgcac atcctgaggc actcccccga ggcgacggag atctgcgtgg agcgggtgaa
     4201 gccattgcgg cgcgccgagg cgagcgggaa ctacgacact ttctacaaga cgttcaatga
     4261 acgggcggcg tccgcgcacg agacactgta aaggagacgt cgcgtgataa tagcaccagc
     4321 ttgagtgggc cagccgaccc gacgggatta cttttgataa cacgcaaaat cgtacgcgat
     4381 cccaagggtg ggcggcccta aaggaagaag ttggccgcag aaatcgagat cgttcgtcag
     4441 aacggcacgt cctggtcagt cactgctgtc tcgtcgtagg tgccgctcgt agcttctgca
     4501 tggccgttgc cctttgaatc caggaactca atcctgtcca cgtagacgag atccctgaag
     4561 tgcttggcac ccgtctgttt gtcgtcccaa gactcggtgc gcttccgacc ctccacgtat
     4621 atgcgggttc ctttgcggag gtgttcgccg acgacctccg cacggcgacc gaaggccttc
     4681 acggcaaacc actccgtgtg ttcttgcagt tcgccgccct tgtccttcca tcgttccgtg
     4741 gtcgcgacgg aaaattgcgc tatggctgtt ccttgttggc tgtatttcac ctcaggat
//
LOCUS       DASRKK010000707         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_951161, whole genome shotgun sequence.
ACCESSION   DASRKK010000707 DASRKK010000000
VERSION     DASRKK010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951161"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1617)
                     /locus_tag="VFC15_17140"
     CDS             complement(247..1617)
                     /locus_tag="VFC15_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM11936.1"
                     /translation="MIGRQNNDQGPLFYEFCLDEAVPDDHLVRKIDAVLDLSWVYAEL
                     APHYPTLGRPSIDPVLMIRMLIIGYVFALRSERLLCREVQVNFAYRWFCKLGIEHKIP
                     DHSAFSRARNERFRDSGIFRRVFERVVEACIAADLVGGEGFAVDASLIQADANKQRSI
                     PGSEWQKTCDPETASRAMQEYLATLDDAAFGAASDTTPKFVSPSDPAAQWTGAMRGPA
                     FFAYADNYLIDVKFGVIMDVEASRAIRQAEVGAAKTMIERTEERFDIKPKLLAADTAY
                     GSGKNLNWLVKDKEIAPHIPVIDKSKRDDGTFSRDDFTFDKERNVYTCPAGKTLTTTG
                     KIHNDETIVYRASTYDCGTCPLKPKCCPKMPERKIPRSIYEEARDVARALAKTPAFEQ
                     SRCDRKRVEMLFAHLKRILKLGRLRLRGPRRAQDEFTLAAIAQNLRPLAKLVVRPPPA
                     VAACGA"
     gene            complement(1898..2500)
                     /locus_tag="VFC15_17145"
     CDS             complement(1898..2500)
                     /locus_tag="VFC15_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11937.1"
                     /translation="MFHDGTNGWQRRYIAGIGILCDDPLKQLDYIMTLRSLSAGNAMR
                     SASFVLVFWLVNASGHTQGASPFDQLSGQWSGSGTIDLANGAIEPIRCRATYDVLEQQ
                     NNLQLNIRCASDSYSFDMRASATLASNAVSGSWSESSRNVAGKISGTADGNLIDVVAN
                     GPAFSASLALNTNGDLQSVVIKSKEANASVKGATISLHRS"
BASE COUNT          728 a          952 c          929 g          726 t
ORIGIN      
        1 agcatatatt cggtacccaa tattgtactt cccggacccg tctctgtgag gccatcaagt
       61 ccggtttgcc ccccaaaagc agacatccag cggatgggtt tgtacgtccg ctaagtgccg
      121 attttgttgc agaagtcggc gatcagtgca tttgggcgct gaatcgattg tttttgaagt
      181 tgtcggttgc tgaccgctcc attgggagcg gcggctttga cgctatggca ccgacaccga
      241 caacggctat gccccgcacg cggcgactgc tggtggtggt cggacgacca gcttggcgag
      301 cgggcgtagg ttctgtgcga tagctgcgag cgtgaactcg tcctgtgcac ggcgcgggcc
      361 gcgcaaccga aggcggccaa gcttcaggat gcgtttcaaa tgtgcaaaca gcatctcaac
      421 acgcttcctg tcacagcgtg attgctcgaa tgcgggagtc ttggccagtg cccgggcaac
      481 gtcacgagcc tcctcgtaga tgctgcgcgg gattttgcgc tccggcattt ttgggcagca
      541 ttttggtttg agcggacaag tgccacaatc ataggtgctt gcgcgataga cgatcgtctc
      601 gtcattgtgg attttgcctg tcgtcgtgag ggttttgccc gccgggcagg tgtagacgtt
      661 gcgctctttg tcgaacgtga agtcgtcccg actaaaggtc ccatcgtcgc gcttcgactt
      721 atcgatcacc gggatgtgcg gcgcgatctc cttgtctttg acgagccagt tgaggttttt
      781 acctgaaccg taggcggtat cggccgcgag cagcttgggc ttgatatcga agcgctcctc
      841 ggtgcgctca atcatggtct tcgccgcgcc gacttcggcc tggcgaatgg cgcgcgaggc
      901 ctcgacgtcc atgatgacgc cgaacttcac gtcaatgaga tagttgtcgg cgtaagcgaa
      961 gaatgccggc cctcgcatcg cacccgtcca ttgcgcagcg ggatcggacg gcgagacgaa
     1021 cttcggggtg gtgtcgctcg ccgcaccgaa ggccgcatca tcaagggttg ccagatactc
     1081 ctgcatggcc cggctcgccg tctcgggatc acatgtcttt tgccattctg agcccgggat
     1141 cgagcgctgc ttgttggcgt cggcctggat caggctcgca tccactgcaa acccttctcc
     1201 gccaacgagg tcggccgcga tacacgcctc aacgacacgc tcgaatactc ggcgaaagat
     1261 gccgctgtcg cggaagcgct cattgcgtgc acgcgagaat gccgaatgat cagggatctt
     1321 gtgctctatg ccgagcttgc aaaaccagcg ataggcgaag ttcacctgca cctcgcgaca
     1381 cagcaaccgc tcggatcgca gcgcaaagac atagccgatg atcagcatcc ggatcataag
     1441 caccggatcg atcgaaggtc gcccgagcgt tggatagtgc ggcgcgagtt ctgcataaac
     1501 ccacgacaga tcgaggacgg cgtcgatctt gcgtactagg tgatcatcag gaactgcctc
     1561 atcgaggcag aactcgtaga acagaggacc ctggtcgttg ttctgacgtc ctatcatcgc
     1621 ccgtctccga cgctaaatgt tgtcagaaga cggaatcact gttcgccaag cccttcaaat
     1681 cacttctgca acaaaatctg ccagtaggcg acattgcgcc tgtggctcaa aataaataaa
     1741 gagaccgcta ttgtgttgcc tagatcgtga aggcgaatgg ctgcgcaaat ctactccgcc
     1801 cagtatttgg tgtttggaac atctaccgga cgtcccgggc gttcgcggca cgcgggcgat
     1861 tgaacacctt gatacaggcg ggaccgagtc gcggcgatta gctgcggtgg aggctaatgg
     1921 tagcgccctt cacgctggcg ttagcttcct tggatttgat aaccaccgac tgcaggtcgc
     1981 cgttcgtatt caaggccaga ctggcggaaa acgccggccc attggccacg acatcgatga
     2041 gatttccatc cgctgtgccg gaaatttttc cggcaacgtt ccggctggat tcgctccaac
     2101 tgccactgac ggcgttcgat gccaaagtcg cgctggcgcg catgtcaaaa ctataactat
     2161 cgctggcgca gcggatattc agttgaagat tattttgctg ctccaacacg tcataagttg
     2221 cccggcaccg gattggctcg atcgcgccat tcgcaagatc aatcgtaccg cttcccgacc
     2281 attgacccga tagctgatcg aacgggcttg cgccctgtgt atgcccgctt gcatttacca
     2341 accaaaatac aagaacgaaa cttgcgcttc gcatcgcgtt gccagcggat agcgatcgga
     2401 gagtcatgat ataatccaat tgtttgagcg ggtcatcgca aagaatgccg ataccagcaa
     2461 tataacgcct ctgccatcca ttggttccgt catggaacat cgggaccgca aggccgcttc
     2521 gaatgcgacg agccagcgtt gcgcgtcggc ttgcgacgga tcccgctgac gatcctcgac
     2581 gatagctatc gcaagacctg tgcactcggg gcgctattga agcattcgac atgcatccta
     2641 tggcaaacgc tacaacgcgc accactcgtc ggccgtcacg gcgctgcatc atcgctaagg
     2701 gcttgcgaac taaaaataaa cgccggaaat tcgctctgca ctgctgcgag cccgacgcgc
     2761 tcgcatagca gtggccgctt cgcaggcgat aagccaagat gaatttaggc tcagcgcctt
     2821 caaggaccgc ttcgggtcaa aggcagacgt gacgctcttc aattgcgatg tctgtattac
     2881 atccaaaagc agacatcccg gcataggctg cgcgagcagt catgttccgt aacctgctgt
     2941 tctatgaatc tctgactctg caatggcgcg aacatagaac cgagccagga acagctttcc
     3001 agacggtaga gacaaaaggc ccaaatccgc ctccaagcta ctttacgtat ttgcagagac
     3061 cgcacctcgc gaatcttaac ccgcaaaagc gcggaggttc gaggcctttg cgatcgcgct
     3121 agagactttg gcggctaaga agcctgcgtg gtggcggacg cagtcctagt cgaacccgtc
     3181 tccactccca aattccctgc taacagggaa ataaacaggg aatttttcaa ttttgggcta
     3241 gctcacgcct cagaggttgc cattcgtcca atgattcagc agacatagag caagattccc
     3301 tactcaacgg aacagggaat tattgcgaag gaaca
//
LOCUS       DASRKK010000708         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_462939, whole genome shotgun sequence.
ACCESSION   DASRKK010000708 DASRKK010000000
VERSION     DASRKK010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462939"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFC15_17150"
     CDS             <1..453
                     /locus_tag="VFC15_17150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM11938.1"
                     /translation="IGDQGRGLLHFIGRRRSLPFSVWIRKRIFPGAYAPTLRESMEVL
                     EPQACYVLDIEDLRLHYARTIEHWLSRFESAYGAVVRDQGTNFARMWRLYLAGSIAAF
                     RVGTLQLFQVVFAGAQCKSAPWTRSRLYTGASQDQDKADDELWTPAMS"
     gene            432..1589
                     /locus_tag="VFC15_17155"
     CDS             432..1589
                     /locus_tag="VFC15_17155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM11939.1"
                     /translation="MDSCDVLIVGGGPAGSSCAWGLRESGLDTLLIDRSTFPRNKLCG
                     GWITPLVFEALGIDPESYAPGRVLQPITGFRLSCIEEPQVDIRYGHTISYGILRCEFD
                     EYLLRRCGARICEGVPLDSIQRTKHGWLVNGDIKARLVVGAGGHFCPVSRFLGNKASE
                     PSVVAREIEFEMPAAQAAGCSISGELPELYFCRDMRGYGWCFRKQNVLTVGIGRMDNH
                     GLSQHCSEFLRFLKQSRNVEVPGKGILGHAYWLFGHSQRAILDDSILLIGDAAGLAYP
                     ESGEGIRPAIESGLIAAHSIISAQGDYSAARLELYRELLNARLGHKRNALESLAQFIP
                     QSVREICGRTLLKNGSFCRNIVCDRWFLRVDERPLAFKSEVSSPVVATAAQ"
     gene            complement(1792..2586)
                     /locus_tag="VFC15_17160"
     CDS             complement(1792..2586)
                     /locus_tag="VFC15_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZM11940.1"
                     /translation="MARQIESPSTAVERTLLILEAAGQREGGMSNADFSRKLKIPKSS
                     ASYILRTLEQHGYLRRDQDDGKYRLGMKVLSLSRAALSGVDVREAALPIMKHLVDHIH
                     ITTHLAILDHGEAVYVEKVEAPSFIKMDTWIGRRMEVHSTAVGKALLAYLDVEQREAI
                     IRQRGLKKLTPHTIISVPKIMKEFERVRQLGYAVDDEENSLGARCVGAAIFNSQGIVE
                     ASVASTGTINAVPRDAVPRLAEMVKDAARRISHQIGYRGPYPPAAR"
     gene            3286..>3579
                     /locus_tag="VFC15_17165"
     CDS             3286..>3579
                     /locus_tag="VFC15_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11941.1"
                     /translation="MAAHQTAAELAKSWKTDPRWNGVARPYSAEDVVRLRGTLPIEYT
                     LAHVGAERLWELLNSEKYVAALGAMSGNQAVQMVSAGLKAIYVSGWQVAADANN"
BASE COUNT          705 a         1074 c         1039 g          761 t
ORIGIN      
        1 atcggtgacc aaggccgcgg actgctgcat tttatcggtc gccgccgcag tctgcccttc
       61 agtgtctgga tccgaaagcg catattcccc ggcgcctacg cacccaccct ccgcgagtcg
      121 atggaggttc tggagccgca ggcctgttac gtgctcgata tcgaggacct tcgtctgcac
      181 tacgccagga ccatagaaca ctggttaagc agatttgaga gcgcttatgg cgccgtcgtc
      241 cgcgatcaag ggacgaattt cgcgcgcatg tggcggctct atcttgccgg atcaatcgct
      301 gcctttcggg tcggcacgct tcagttgttc caggttgtgt ttgctggggc ccagtgtaag
      361 tccgctccat ggacgcgcag ccgcctgtac accggcgcca gccaggacca agataaggca
      421 gacgacgaat tatggactcc tgcgatgtct taattgtcgg cggcggcccg gcgggatcct
      481 cctgcgcctg gggactgcgg gaatccggac ttgacaccct gctgatcgac cgcagcacct
      541 tcccgcgcaa caaactttgc ggaggatgga tcacaccgct ggtgttcgag gcgctgggca
      601 tcgatccgga gtcctacgcg ccggggcgtg ttctgcaacc gatcaccggc ttccgcctga
      661 gctgcatcga agagccgcaa gttgacatcc gctacggtca caccattagc tacggcatct
      721 tgcgctgtga gtttgacgaa tatctcctgc gccgttgtgg agctcgcatt tgcgaaggcg
      781 tcccgctcga ttcgatccag cgaactaagc atggctggct cgtcaatggc gacatcaagg
      841 cacgacttgt ggttggggcc ggcggacact tttgtccggt gtcccgcttc ctcgggaaca
      901 aagcctccga gccgtcggtc gtcgcgcgcg aaatagagtt tgaaatgccc gccgcgcaag
      961 cggctggctg cagcatcagc ggcgagttgc ctgagcttta cttttgccgc gacatgcgcg
     1021 gctacggatg gtgtttccgc aaacagaatg tcctgaccgt cggaatcggc cgcatggata
     1081 atcatggcct ctcccaacac tgcagtgagt tccttcgctt tctcaagcaa agcagaaatg
     1141 tcgaagtgcc cggcaaaggc attttgggac acgcgtactg gctctttggg cactcgcaac
     1201 gggcgattct ggatgactcc atcctgttaa tcggggatgc cgcgggtttg gcctacccgg
     1261 aaagcggcga aggaatacgg cccgctatcg agtcaggatt gattgccgct cacagcatca
     1321 tctccgcaca gggcgattac agcgccgccc gcttggaact ctaccgcgag cttttgaatg
     1381 cccggttggg ccataagcgg aacgccttgg agtcgctggc gcagttcatc ccgcaatcgg
     1441 ttcgcgagat ttgcggccga actctgctga agaacggttc gttctgccgc aatattgttt
     1501 gtgaccgttg gtttcttcgc gtagatgaac gcccgctcgc cttcaagtcg gaggtctcct
     1561 caccggttgt ggcaacagct gcgcagtaag aacggccgag tggaagtgag aaccattcag
     1621 cattcagcat tcagcaaatg cattgtcatc ctgagcgagc ccgccgcggc gggcgagtcg
     1681 aaggacccct atgccagccg agcttcttac cagggatagg ggttcttcgg ctgcgctcga
     1741 tcgctttcgc gacctcgctc cgctcagaat gacagcaaca aacgaaatat cctaccgagc
     1801 tgctggcgga tacggccctc gataaccgat ctggtgtgag atgcggcggg ctgcgtcctt
     1861 caccatctcc gccagccgag gcacagcgtc acgcggcacg gcattgatgg tcccggtgga
     1921 tgcgacgctg gcttcgacga tgccctggct gttaaagatg gctgctccca cgcagcgcgc
     1981 acccagactg ttctcctcgt catccacggc ataccctaac tgacgaactc gctcgaactc
     2041 tttcatgatc ttgggtacgc tgatgatggt atgcggcgtc agtttcttca gtccccgttg
     2101 gcgaatgatc gcctcccgct gttcgacatc caggtatgca agcagcgcct tgcccaccgc
     2161 cgtcgaatgc acctccatcc gccggccaat ccaggtgtcc atcttgataa agctgggcgc
     2221 ttcgaccttc tccacgtaca ccgcttcgcc gtggtcgaga attgcgaggt gagttgtgat
     2281 gtgaatatga tcgaccaggt gtttcatgat ggggagcgcc gcctcgcgca catcgacgcc
     2341 gctcaaggcg gcgcgcgaca ggctcaacac cttcatgccg agccggtact tgccatcgtc
     2401 ctggtcgcgg cgcagatagc catgctgctc cagagtccgc aaaatgtagc tggctgagct
     2461 cttgggaatt ttgagcttgc ggctgaaatc cgcgttcgac atgccgccct cgcgctggcc
     2521 ggcagcttcc aggatcagca gcgtacgctc taccgcagtc gaggggctct caatttgacg
     2581 cgccatagcg accagtctag cagcggacag ttgtgcgaca tagtgaattc gagttcataa
     2641 tcccggccat tgccaagcta cgtttgaaca ggctgcggaa aaactctggg caaaactcgt
     2701 ggagcgtact tcttggtgga gccccgcatt ttatgcgggg gaaggagcgc ttcggcgctc
     2761 cgggaaacgt tcggctttga tcgcgcgctt cagcgccgga gatcgaaaca atcccgggct
     2821 agaagcccat ttcaagatcg acccttttcc gctggactga agtccagctc cccctgctga
     2881 agcagggggg cttccaccaa aagcctcccc cccaagtttt ttcgcagctt gtgaagccgc
     2941 ctccgttaaa caaggtgggt cacccaaact ctcgagtgag ctatatgtca cagacaaagc
     3001 agggcggctg cacttagcct aaagccagcg ctccggtcca ggtgccactc gagtgagcaa
     3061 cccgcttccc agtatgggaa tcgccaaccc agagtgcaca atggcgaatt tgtgttcggt
     3121 attctgaaca ctgttcttgc cttcgtatcg ggagaccgct atggtttgta caccatgcgg
     3181 aataagtgtt cagcatggtg aacaggctga ccacatggcc ggcagtctgt acttaacttt
     3241 ggtcgttaga cccttcttat acccgaactt gagaggagaa cgaagatggc ggcgcatcaa
     3301 actgctgctg aacttgcaaa atcctggaag accgatccgc gctggaacgg ggtcgcccgg
     3361 ccctattccg cggaagatgt tgtgcgcttg cgcggcaccc ttcccatcga atacacgcta
     3421 gcccacgtag gcgccgagcg cctgtgggag ctgctgaaca gcgagaagta cgtcgccgcc
     3481 ctgggcgcca tgagcggcaa ccaagcggtg cagatggttt cggccggact gaaggccatt
     3541 tacgtgagcg gctggcaggt cgccgccgac gccaataac
//
LOCUS       DASRKK010000709         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_73329, whole genome shotgun sequence.
ACCESSION   DASRKK010000709 DASRKK010000000
VERSION     DASRKK010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73329"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFC15_17170"
     CDS             complement(<1..644)
                     /locus_tag="VFC15_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM11942.1"
                     /translation="MSRSKSESLLGLTESFFHNYLQQTRGASGHTVRAYRDALKLFYL
                     FLADQKRKPVADLALDDIQSDALLAFLDHVESRRGNSAVTRNCRLAAVRSFVQHLLRH
                     DVTRAGQYGRILAIRNKKALRRAATYLEPEQARAVIAAIDRRSRNGERDHALLLFLYN
                     TGARVSEALAIRTCDLQLDRPRQVRLLGKGRKERICPLWPETASALHRIIPAKSG"
     gene            complement(641..1612)
                     /locus_tag="VFC15_17175"
     CDS             complement(641..1612)
                     /locus_tag="VFC15_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11943.1"
                     /translation="MINIFQGPTAREMHAFLQFKRSLGYGYMRAEFTLREFDRFLIQY
                     AANNRRLKLDRAAIAWLSSKPGRKPISVSADAAVLRQFFRYLRRSSEPGTVAEPIWPH
                     LPTESSFLPYSLSEKDVLHLLALCADLKRPPFRATLYRALILVLYCTGIRFGEALRLR
                     MRDVDTRSAVLFIDTFKGRSRWVPFHRSLSRELDQYLTARLAYAPSDMGARFFVGTNQ
                     RRLPVETAARTFRILFKLAGLKPERGRIGPRPYDLRHAFAVRRLSRWYGEGVDLHARL
                     PWLSAYMGHVDIIGTESYLHTTPELLDLAGNRLRRRYKNTTSEDDSV"
     gene            complement(1609..2817)
                     /locus_tag="VFC15_17180"
     CDS             complement(1609..2817)
                     /locus_tag="VFC15_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11944.1"
                     /translation="MRVIDCRERAVVGLWRKGHLSLGTIVIYLQWVRRFRTYCDKRKL
                     PETEQLTAVGARRFAKAYAGPRLKGRQSSKESRNLASNALHAWACASAALGTPLLPWR
                     DKHPPTLPPLLEEYCHYRRAHNGVSERTLVRDVETARGFLEQLRSGRTTIARARLTDV
                     DAFVRKLATRLSRRTVADTCSSLRGFLRFLRITGRLTADLANEVVAPRYRIDERPSRC
                     LPWSDVQKILRAISRTEAPGKRDYAIVLLLATYGLGAAEVLGIRLEDVNWQGGVLRVC
                     RPKTNVSIELPLLPAVAQAMTAYLRWERPPARPVQRIFVRENMPYEPLTSGAIRHRIR
                     HYAHLAGISTKVIGAHAFRHSHARRQIDFGANVKIVSDILGHRSSSSTSVYVRVALER
                     LRAVALPVPR"
BASE COUNT          659 a          889 c          906 g          605 t
ORIGIN      
        1 ccgctttttg cgggaatgat ccgatgtagg gctgatgcgg tttcgggcca aaggggacag
       61 attcgttcct ttcggccttt gcccagaagc cgcacttgcc gtggcctgtc caactgaaga
      121 tcacaggtgc gtatcgccaa cgcttcgctg acgcgcgctc ccgtgttata gagaaacagc
      181 agcagggcgt gatcgcgttc cccattacga ctccgacgat caatggcggc gatcaccgcc
      241 cgggcttgtt caggttccag ataagtggcg gcccgtcgta gtgccttttt gttgcgaatc
      301 gcgagaatgc gaccgtactg accagcccgc gttacatcgt gccgcagaag gtgctgcaca
      361 aaactgcgaa ccgcagcaag gcggcaattc cgggtaaccg cggagttccc tcggcgcgat
      421 tcgacatgat ctaagaatgc gagcagggcg tcggattgga tatcgtccaa ggcgaggtca
      481 gccacgggct tgcgcttctg atctgccagg aacaagtaga aaagcttcag cgcatcgcgg
      541 taggctcgca cggtatgccc acttgctccg cgcgtctgtt gtaaatagtt atggaagaaa
      601 ctttcggtca gacccagcag cgactcagac ttggagcggc tcatacactg tcgtcctcgg
      661 atgttgtgtt tttgtatcgg cgccgcagcc ggttgccggc caggtccagg agctccggcg
      721 tggtgtgcag gtaactctca gtgccgatga tgtcgacatg tcccatatac gccgatagcc
      781 agggtaggcg ggcatggagg tcgacacctt caccgtacca tcggctcagg cggcgaacgg
      841 cgaaggcatg gcggaggtca tagggtcgcg gcccgatccg acccctttcg ggcttcaacc
      901 cggccagttt gaacagaata cggaaagtgc gagctgcggt ctcaaccggc agccggcgtt
      961 ggttcgtccc aacgaagaag cgagccccca tgtccgatgg cgcgtacgcc aggcgagcgg
     1021 tgagatattg atcgagttct cgtgatagcg aacgatgaaa cggaacccat cgggatcgtc
     1081 ccttgaaggt gtctataaag agaacggcgg atcgagtatc cacgtctcgc attcgcaggc
     1141 gcagcgcttc tccaaaacga attccggtac agtacaggac cagaatcaac gccctgtata
     1201 gtgtggcccg gaagggcgga cgcttgaggt cagcgcagag cgccaggagg tggagaacgt
     1261 ccttctccga aagggaatac ggaagaaagg aggactcggt aggcaggtgc ggccagatcg
     1321 gttcagctac agtccctggc tcggatgatc ggcgcaggta tcggaaaaac tgccgcagca
     1381 ccgcggcatc ggcggagacg ctgatgggct ttcggcccgg cttgctcgac agccaggcaa
     1441 tcgcggcgcg atcaagtttc aagcggcgat tgttagccgc atactgaatc agaaaacgat
     1501 caaactcgcg caacgtaaac tccgcacgca tgtagccata gccgaggctc cgcttaaact
     1561 gtagaaaggc atgcatctca cgcgcagttg ggccctggaa gatattgatc atcgcggcac
     1621 cgggagagca accgctcgga gacgctccaa cgctactcgc acatagacag aagtggagga
     1681 cgaactgcgg tggccgagaa tgtcgctcac aatcttgaca ttggccccga aatcaatctg
     1741 cctgcgggcg tgactgtgac gaaaagcatg ggccccgatc actttggttg agatgccagc
     1801 cagatgcgca tagtgccgga tacggtggcg aatggcgccg ctggttagcg gctcgtaggg
     1861 catgttttcc cggacaaaaa tacgttgaac gggtctggca ggcggccgct cccatcgcag
     1921 gtatgcagtc attgcctgtg caactgccgg caacagagga agctcgatcg acacattggt
     1981 cttcggccga cagaccctca gcactccgcc ttgccagttc acatcttcca atcgaattcc
     2041 aaggacttcg gctgcgccca gaccatacgt tgccaacagc aacacgatcg catagtcacg
     2101 cttgcccggc gcttccgttc gagagatcgc acgcaggatc ttctgcacgt cgctccacgg
     2161 caaacagcgt gaggggcgtt catctatccg atatcgcggt gcaaccacct cgttggccag
     2221 gtcggccgtc aatcttccag ttatccgcag aaaccggaga aaccctcgga gcgaactgca
     2281 ggtatcggcg acggttcgtc tcgaaagacg ggtggcaagc ttccgcacaa aagcatccac
     2341 atctgtcaat ctcgctctcg caatggtggt ccttccactg cgcagttgct cgaggaagcc
     2401 acgcgcggtc tcgacatcgc gcaccagcgt ccgttccgag accccattgt gggctcgccg
     2461 ataatggcag tactcctcca gtaacggtgg gagtgtcggc gggtgtttat cgcgccacgg
     2521 cagcagcggc gtgcccagcg ccgccgacgc gcaagcccac gcatggagcg cattgctggc
     2581 gaggttgcgg ctttccttgg aactttgtcg cccttttagg cgaggtccgg catacgcctt
     2641 agcaaatcgg cgcgccccaa ccgcagtcag ttgttcggtt tcgggtagtt tccgcttgtc
     2701 gcaatatgta cggaaccgac gaacccactg gaggtagatg acgattgttc cgagtgaaag
     2761 atgacccttg cgccacagcc caacgaccgc gcgttcgcgg cagtcaatca ctctcactgg
     2821 atccttcatg caatattgga cgtctgtcac cggagagaat tatcacgacg cccacgagcc
     2881 attcaccgcg ttctacgcac ctttcaggca ttcactctgg ataattccag agtccagata
     2941 atgagcgttg cagttcgcca ccagtgtggg gttgagtttc ggcacattcc tcgatagcgc
     3001 atcgcacatt tgaatggggg gcgaaaggcc cggaggccgc tgtttcagca cctccgcca
//
LOCUS       DASRKK010000710         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_267007, whole genome shotgun sequence.
ACCESSION   DASRKK010000710 DASRKK010000000
VERSION     DASRKK010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267007"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="murD"
                     /locus_tag="VFC15_17185"
     CDS             <1..1038
                     /gene="murD"
                     /locus_tag="VFC15_17185"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZM11945.1"
                     /translation="TTTLAGEIIAAGGRKALVGGNIGTPAITFVESSTEDTWVVLEIS
                     SFQLETIESFHPRIAVVLNVTPDHLDRHYSFENYAAAKARIFENQSPNDFAVLNADNQ
                     PCIEMASAAKASVSWFSRVKEVTSGAFVRGQYIVWRDSHGDREIMSVREISLKGTHNL
                     ENVLAAVCVGMLAGIEPAQIRCAVSEFKAVEHRLEFVATVRGVEYYNDSKATNVDATI
                     KALESFPGHIHLILGGKDKGSDYSVLNALLKERVKRVYTIGAAAAKIESQAQGTEIAS
                     VGTLEAAVKRASESASPGDVVLLAPACASFDQFDSYEHRGRIFKDLVRQLASREQQRE
                     HVGSAARGGNE"
     gene            1038..2129
                     /gene="ftsW"
                     /locus_tag="VFC15_17190"
     CDS             1038..2129
                     /gene="ftsW"
                     /locus_tag="VFC15_17190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZM11946.1"
                     /translation="MAKRVSVDKWLFTVTLLLVFIGLVMVFSASAVMAKERFGSPYGF
                     LLRQMAWAGAGIAAMLVIMHIDYRKYRRASVVFTFLGFTSLLLIAVFLFRDSHNTHRW
                     IKLGGFSLQPSELAKPAIIFYLAYFLESRWQQITDWKNVLLPAAAPVVLFALLIVKEP
                     DLGTAIACLAISAAVLFVAGMNMKYLWYALAASLLPLYFLVVRVPWRWARVQAFLDPF
                     KDPQGKGFHIIQSMIAVSTGGLTGLGLMEGKQKLFYLPEPHTDFIFAVTAEEMGLWGS
                     LLVVTLFGIFLYRGIRTALRTDDMFGKFLATGFTAMVVVQALFNISVVLAMMPAKGIP
                     LPFVSYGGSSLFITLASVGVLLNITQQTD"
     gene            2132..3229
                     /gene="murG"
                     /locus_tag="VFC15_17195"
     CDS             2132..3229
                     /gene="murG"
                     /locus_tag="VFC15_17195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZM11947.1"
                     /translation="MRVIVAGGGTGGHVIPALAIAQELRSRYHAQVLFVGTQRGIETR
                     LVPAAGFELHIIQVGALNRVDFATRLKTLLDLPHAFIESAKLIREFRPDVMIGVGGYA
                     SGPAMLTAAMMNVPTVAFEPNVIPGFANRIVAPMVSVAAVHFEATCHYFRNCHVTGVP
                     VRQEFFHVPARPPVRSKDVRPTLLVFGGSQGAHAINQAVLDALPKLMEAVPAIHVIHQ
                     TGEKDYVHAQSVYLRTMISAEVSAFIDNMPGAFARADLLVCRSGASTVAEITAAGKPA
                     IFIPLPTAADDHQTQNAATLADGGAARLLPQSELSSDRLVNEIVSLLRDRSRLAAMSE
                     AARRFAHPDAAAKIASLAARAAGVQSQHAVA"
BASE COUNT          594 a          948 c          986 g          725 t
ORIGIN      
        1 accacgacgc tggcgggcga aatcattgcg gctggaggcc gtaaggcgct ggtcggcgga
       61 aacataggaa ccccggcgat caccttcgtc gagagttcga ccgaggacac ctgggtggtg
      121 ctggagatca gcagcttcca actggagacc atcgagagct ttcaccctag gattgctgtg
      181 gtgttgaatg tcacccccga tcaccttgac cggcactatt cgttcgagaa ttacgccgcg
      241 gccaaggctc gtattttcga gaaccaaagc ccgaacgatt tcgccgtttt gaacgcggac
      301 aaccagcctt gcattgagat ggcctcggcg gcgaaagctt ccgtgtcctg gttcagccga
      361 gtgaaggaag tcacgagcgg ggcgtttgtc cgcggacagt acatagtctg gcgcgacagc
      421 catggcgacc gcgagatcat gtctgtccga gagatttcgc tgaagggcac gcacaatctg
      481 gaaaatgtgc tcgcggcagt ttgtgtggga atgctggcgg gaatcgagcc ggcacaaatc
      541 cggtgcgcgg tctccgagtt caaagcggtg gagcatcgcc tggagttcgt ggccacggtt
      601 cgcggcgtcg agtattacaa cgattccaaa gccactaacg tggatgcgac catcaaggcg
      661 ttggagtcgt ttcccggcca cattcacctc attcttggcg gcaaagacaa aggcagcgac
      721 tacagtgtgc tgaatgcgtt gctcaaggag cgagtgaagc gcgtctatac cattggtgcg
      781 gccgccgcca agatcgagtc gcaggcgcaa ggaactgaga tcgcaagcgt cgggacgctg
      841 gaggccgcag tcaagcgcgc cagtgaatct gccagtccag gcgatgtagt gttgctggct
      901 ccggcctgcg ccagcttcga tcaatttgac agctacgaac accgcggacg gatcttcaaa
      961 gatctggttc gccaactggc atcgagggaa cagcagcgag agcatgtcgg cagcgctgcg
     1021 cgcggaggca acgaataatg gccaagcggg tcagcgtgga taagtggctg ttcacggtta
     1081 cgcttttgct tgtctttatc ggactggtga tggtgtttag cgcctcggcg gtaatggcga
     1141 aggagcgctt cggttcgccc tatggttttt tgctgcggca aatggcgtgg gccggcgccg
     1201 gcatcgcggc catgctcgtc attatgcaca tcgactaccg gaaataccgg cgggcgtcgg
     1261 tggtcttcac ttttctggga tttacctcgc tgctgctaat cgccgttttc ttgtttcgcg
     1321 attcccataa tacccaccgc tggatcaagc ttggcggttt ttccttgcag ccttcggagc
     1381 tggcgaaacc cgcaatcatt ttttatctcg cgtactttct ggaatcgcgc tggcagcaaa
     1441 tcaccgactg gaagaacgtg ctgcttccgg ccgccgcccc ggtagttttg ttcgcgctgc
     1501 tcattgtgaa ggagcccgat ctcggtaccg cgatcgcgtg cctggcaatc tccgccgccg
     1561 tgttgttcgt ggcggggatg aacatgaagt atctgtggta tgcactcgct gcgtcgctgc
     1621 tcccgctgta ctttctggtt gtccgcgttc cctggcgctg ggcgcgcgtg caggcattcc
     1681 tcgatccctt taaggacccg cagggcaaag gcttccacat tatccagtcc atgatcgccg
     1741 tgagcaccgg aggcctgacc ggcttggggc tgatggaagg caagcaaaag ctcttctacc
     1801 tgccggaacc gcataccgac ttcatcttcg ccgtcaccgc ggaagagatg ggattgtggg
     1861 gctcgctgct ggtggtcacg ctcttcggta ttttcctgta tcgcggcatc cgcaccgcgc
     1921 ttcgcacgga tgacatgttc gggaagtttc tggcgaccgg cttcaccgcg atggtcgttg
     1981 tgcaggcgct gttcaacatc agcgtggtac ttgccatgat gccggccaag ggaattccac
     2041 tgccgttcgt ttcctatggc ggctcttcgc tcttcatcac tctggctagt gtcggagtcc
     2101 tgctcaacat cacccaacag acggactagc catgcgtgtg atcgtggccg gaggtggaac
     2161 tggcggacat gtgattcctg cgctggccat cgcgcaggag ttgcgttccc gataccacgc
     2221 gcaggtgctg ttcgtcggca cccagcgtgg cattgagacg cggctggttc cggcggcggg
     2281 tttcgaactg catatcatcc aggttggcgc acttaaccgc gtggatttcg cgacccggct
     2341 taagactttg ctggacctcc cgcatgcctt catcgagtcg gccaagctaa tccgcgaatt
     2401 tcgacccgat gtcatgatcg gtgtgggcgg ctacgcttcg gggcctgcga tgctgaccgc
     2461 tgcgatgatg aacgtaccca cggtcgcctt cgaacccaac gtgatacccg gcttcgccaa
     2521 tcgcatcgtc gctcccatgg tgagtgtggc ggcggtgcac ttcgaggcga cgtgtcacta
     2581 ttttcgcaat tgccatgtga ccggcgtacc cgtacggcag gagttcttcc atgttccagc
     2641 gcgtccccca gttcgttcta aagatgtccg ccctacgcta ctggtttttg gaggtagtca
     2701 aggcgcccac gccatcaacc aggccgtgtt ggatgctttg ccgaaactga tggaggcggt
     2761 gccagcaatc cacgtcatcc atcagactgg cgagaaggac tacgttcatg cgcagtcggt
     2821 ttatttgcgc accatgattt cggcggaggt gtccgctttc attgataaca tgccgggggc
     2881 cttcgcccgc gctgacctgc tggtttgccg ctctggagcc agcaccgttg ccgaaatcac
     2941 ggcggcaggg aagcctgcca tcttcattcc tttaccaacg gccgccgatg accaccagac
     3001 tcagaatgca gcgacgctcg ccgacggggg tgcagcccgc ctgttgccgc agtctgaact
     3061 ttccagcgac cgactggtga acgagattgt ctcgctgttg cgcgaccgct ctcgattggc
     3121 ggcgatgtcg gaggcggcgc gccgctttgc ccatccggac gcagcagcta aaatcgcatc
     3181 gctggcggcc cgggccgctg gcgtccaaag ccagcacgcg gtagcctaga gactcgtctc
     3241 tcccatgttt gcc
//
LOCUS       DASRKK010000711         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_170074, whole genome shotgun sequence.
ACCESSION   DASRKK010000711 DASRKK010000000
VERSION     DASRKK010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170074"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            835..1923
                     /locus_tag="VFC15_17200"
     CDS             835..1923
                     /locus_tag="VFC15_17200"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HZM11948.1"
                     /translation="MRHYNIAVMPADGIGPEVITEGVAVLRAVAELHGGLTFETQNYD
                     WGSERYLRHGAMMHPDALKVLEAGGFNAILVGPVGDPRVADHITLWGMLLPIRQGFDQ
                     YINLRPIRLLPGVAGPLAGKGPTDIDVVCVRENCEGEYCGVGGRVYQGTPQESAVQAA
                     VFTYHGTERVMRYAFEYARRHGRKKVTSATKSNAVNYSMVFWDDVFREVSAKYPDVQH
                     EQQLIDSLCARFVTMPESLDVVVASNLFGDIVTDLGAAIAGSMGLAPSANLNPEGRYP
                     SLFQAIHGSAPGLAGKGIADPVATIWSVSMMLDHLGEAELGAAVMGAVESVIAGGKVR
                     TPDLGGSATTRQMGQAIIAALRKPGMAC"
     gene            complement(1949..2824)
                     /locus_tag="VFC15_17205"
     CDS             complement(1949..2824)
                     /locus_tag="VFC15_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HZM11949.1"
                     /translation="MDSHGVLIYYEAVGRGGALVIVHGGPGASHDYFLPYLLPLMRTN
                     RLVFIDERGSGKSSKLEDPKQYTINNMVEDLEAVRLGLGLGKISLLGHSFGGALVQAY
                     ALKYQKNLSHLILGSTFSSTKELNEALAGIKAGMDSKDRERVAALEAAGLFGQGEVWE
                     HGRYPEEYAKLAWGKGYFPYLYQNRPDPNYDPLSSNTGTAWDVYREMWGSDGEFVVDG
                     NLKEVEYLDRLSEIRVPTLIIVGDHDESDPKMSKEMHAKIAGSQMVILPNSGHMTFVD
                     QPEEFLKTVREFVAH"
BASE COUNT          793 a         1046 c         1048 g          781 t
ORIGIN      
        1 acaaagcatg ccttctagct ccagcagggc actttctccg agtccggttt aagcgcaaaa
       61 cacgcggaat tgcggggatt ttcggcgatt ttcagaggaa agcagccaga tttctctgca
      121 acccagactg cgtggcggag ggagttggat tcgaacttcg aatacagttt caaactatgc
      181 gctaagatcc cgtgtgtcag cgacttaccc ggatttcatt caagaaatga gcacagcgga
      241 gaaacgaatt aggctcggcc acgcgcccga gcagtccgct ttctgtggcg attcaaaggc
      301 gaaggattag cgattattgg gcgatagcgc gctgtggttg agccggctcg actctcaaaa
      361 ggattcgcaa aatgactgct tcctcaccgg acggtccgaa gtgcagaaaa attctccggc
      421 agcgctcgcc cggaaacgca gatttctgcg tattttggac atggtcgccc gcgttttccc
      481 agccgagttc agagaatcgg gcgccgcatc tcagtgacta gagcgtgctt cgaccaaatg
      541 gattgtttcc cgtccgtcat ccgcccagca tcgatgcttg aactttagaa gaagccgacg
      601 tgacgactgt ggaccaacgg tcagcgctgg aaacggcgac gtaatccatt ttccgggcat
      661 agaattgcag ccgtcgtaag ttacctcgca cgccttcggc aaaagtaatc tcccgcacag
      721 ggcagaacaa aatgctgccg acggcgacgg tagcggaata tcaccgggca gcaggagagc
      781 ggctgaacgt caagagaacg tgtctgcaga ccagatagta gtgaggagat actgatgcgg
      841 cactacaaca ttgcggtaat gcccgccgac ggcatcggcc cggaggtcat aacggagggc
      901 gtcgcagtcc tccgggcagt tgccgaactt cacggtgggc tgacgttcga aacccagaac
      961 tacgactggg gctcggagcg gtatcttcgc cacggagcga tgatgcatcc cgacgcactg
     1021 aaggtgttgg aggcgggtgg ttttaacgcc atcctggttg gcccggtggg cgatccacgt
     1081 gtggccgacc acataacgct gtggggtatg ttgctgccca ttcgccaggg ctttgaccag
     1141 tacatcaatc ttcgtcccat acgcttgctg cccggcgtgg ctggcccgct ggccggtaaa
     1201 ggtcccacgg acatcgacgt cgtctgcgtt cgcgagaact gcgaaggcga atattgcgga
     1261 gttggcggac gtgtttatca gggaactccg caggagtcgg cggtgcaggc ggcagtcttc
     1321 acctaccatg gcaccgagcg cgttatgcgc tatgccttcg agtatgcgcg ccggcacggg
     1381 cgcaagaaag tgaccagcgc gaccaagtcc aacgccgtca attacagcat ggtcttttgg
     1441 gacgacgtgt tccgggaggt ttccgccaag tatccggatg ttcagcacga acagcagttg
     1501 attgactcgc tgtgtgcgcg ttttgtcacc atgccggaga gtttggatgt ggtcgtagcc
     1561 tcgaacctgt tcggcgatat cgtcactgat ctcggcgcgg ccattgccgg cagcatgggt
     1621 ctggcgccga gcgcgaacct caatcccgag ggccgttatc caagcctgtt ccaggcgatc
     1681 cacggctcgg cacccggcct cgcaggcaaa ggtatcgctg atcctgtcgc cacaatctgg
     1741 tcagtctcga tgatgctgga ccatcttggt gaagccgaac ttggagccgc tgtcatgggg
     1801 gcggtggaaa gcgtgattgc cgggggaaag gtccgcacgc cggacttggg tgggagtgct
     1861 acgacaagac agatgggaca ggcgatcatt gctgccctgc ggaagccggg gatggcctgc
     1921 taatcgctgc cgcccggctg tagtcacttc agtgcgctac gaactcccgc accgttttca
     1981 gaaactcctc cggctgatcg acgaacgtca tgtggccact gttgggaagg atgaccattt
     2041 gcgatccggc aatcttcgcg tgcatttctt tcgacatttt cggatcgctc tcgtcgtgat
     2101 cgcccacgat gatcaacgtc ggaaccctaa tttcggacaa ccgatccagg tactccactt
     2161 ctttcaaatt gccatccacg acgaactcgc catccgaccc ccacatttcg cgatatacgt
     2221 cccatgcggt gccggtgttc gagctcaacg ggtcatagtt cggatcgggc cggttctgat
     2281 acaggtacgg gaaatacccc ttcccccaag ccagtttcgc gtattcctcg ggataccggc
     2341 cgtgctccca gacttcgcct tgtccgaaca ggcccgcagc ctccagagcg gctacgcgtt
     2401 ctcgatcctt cgaatccatt cccgctttta tcccagccaa ggcttcattc agttccttcg
     2461 tgctcgaaaa cgtgctcccg agaatcaggt gcgacagatt cttctggtat ttcaacgcat
     2521 aagcctgtac gagcgccccg ccgaacgaat gccccagtaa actgatcttg cccaatccaa
     2581 ggcctaagcg cacggcctcg aggtcttcga ccatattgtt gatggtgtat tgcttgggat
     2641 cttccagctt ggaagacttg cccgagccgc gctcgtcgat gaagacgagc cggttggtcc
     2701 gcatcaaggg cagaaggtac ggcaagaagt aatcgtgcga agcgcccggc ccgccgtgca
     2761 cgatcacaag agccccgccc cgccccacgg cttcgtaata aatcagaacg ccatggctgt
     2821 ccacgaagcc ttcctgcacc gtatatacag ggggcagctt cgaagtttga gcgagacaaa
     2881 aaacaggtag gacgagcaga cagagcgaaa ccgcacgctt gcgcatgccc gaaatgctag
     2941 catgcgggcc tcgctcgacg agagcggtgg gaagacgctt gatcgcgatg tcgcggccgc
     3001 tttgcgtgtc gcggtccaga aacgggcttc tgggaagtgt cgatttagga ttccgtcaat
     3061 tcttgggtgg gtggcagcac ggacttcagg taatccaact cttccggtaa cgcggtcaga
     3121 atcgcgaagt caataagagc catggattcc tcgagtccag caaatctatc acgaaaacaa
     3181 atcggccgac acagccgaaa gccttggcgt gcggtcgacc cggggaatga ttcctccctc
     3241 ttgtcgccgg attccgggcc tttcccctcc gctttcataa aggggtggac tcaattccct
     3301 gataagcggc cctgaattcc ctgatacgcc cctgataatt ccctgttcaa ttcaaaccaa
     3361 aaaattgctc ttggcagagg aggttgtcta gaatgaggag tttaccgctg gtcacggccg
     3421 gaatgggccg actaaattta cgaaattccc tgtatttttc cctgttagca gggaatttgg
     3481 ccggagaagg gttagcagca gactgcctcc tccgccagac agtctggact gcagagaaac
     3541 tctgctgcgt tgctccctta ataggcgaga aacgcgggct tttcgcagta ttccctcaac
     3601 aaaccggacg agagaaaatg gcccggtgag ctgcgtccct cccgtttgca gcggttttct
     3661 ccagagcc
//
LOCUS       DASRKK010000712         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_463813, whole genome shotgun sequence.
ACCESSION   DASRKK010000712 DASRKK010000000
VERSION     DASRKK010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463813"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..485
                     /locus_tag="VFC15_17210"
     CDS             66..485
                     /locus_tag="VFC15_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11950.1"
                     /translation="MTIPTTSGDTPNFPEAPTYSAHITEELRRYDEHLRDVRGLAAGT
                     RKGRLRIIGWLLECSFKHREIVFARLRPDDVRQYLVRRLAENPSASNGSQHAAALRSY
                     LRYRATCGDQVGKLVAVCEPSPLAARNPAEGIERRGC"
     gene            490..1023
                     /locus_tag="VFC15_17215"
     CDS             490..1023
                     /locus_tag="VFC15_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11951.1"
                     /translation="MLGTAALARKTPKRAYAIVRLALDLGLRSGEIAHLNLNDIDWQA
                     GTLMLRGTKSLRQDAVPLPEETGKALVDYLRHERPAIHHPAVFVRQVADRMLPITSCA
                     VQKVIKCACRRIGLPDASAHALRHTVACRLVERGSSLKEVADLLRHRSLNSTLIYAKL
                     DTPKLAAVALPWPGSAA"
     gene            1020..1994
                     /locus_tag="VFC15_17220"
     CDS             1020..1994
                     /locus_tag="VFC15_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11952.1"
                     /translation="MSAHISLQQRIDDYLAERQRLGFKLHSRSTLLAGFARYVADRKH
                     RGTLTTDLMIDWAKQDKWQRGTPATWAARLARIRHFARYLKQFEPDTEVPEELTFGPE
                     PGRVAPHIFHEPEVIDLLAAARQLGPTGSIRPATYETLFGLMASTGLRVSEALHLRDA
                     DVDLKRGMLTIRQTKFAKSRLLPVHPTTVAALAKYRKARAAHMATVADMPFLISSRGR
                     RLGKPLGDRQAHRVFNMLRDHLGWANRGGHAAIRLHDLRHTFAVRRLMRWHADGTDID
                     QMMLALSTYMGHAEIFYTYWYLTAVPELMALAGSKFEHFADLIGGCDE"
     gene            1987..3000
                     /locus_tag="VFC15_17225"
     CDS             1987..3000
                     /locus_tag="VFC15_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM11953.1"
                     /translation="MNKHPKLPPSFPALVQAYFGEYLTQQRALSPQTIAAYRDGFVLF
                     LKFAEARLGKAPAAMALADVTPELIMAFLDHLERQRHNSVRSRNARLAALRSFLKFAG
                     HRDIASLQVIERALGVPVKRFERPMFGYLTREEVLAVIGEPGDSWLSQRDHALFLLMY
                     NTGARVSEIIGVKVGDVVLDASAACVHLHGKGRKQRSVPLWQATVKALRAWLRANLEL
                     TAEATLLPNRNGREMTRANVTLRLALSVQRAMETQPALAKRKVSPHTVRHTTAMHLLL
                     AGVDISVIALWLGHESPVTTHHYVEADLAMKERALARLHEPGAKIQRYRAPDSLINFL
                     RTL"
BASE COUNT          639 a          978 c          962 g          685 t
ORIGIN      
        1 cgagcactgg ctcgacataa tcttggtggt gatttgaagt aacggttcca tttcaggaga
       61 ccgttatgac cattccaacg acgagcgggg atacgccgaa tttcccggaa gctccgacat
      121 attcggcaca catcacagaa gaattgcggc gctatgatga acatctacgg gatgttcgcg
      181 ggctggcggc cggtacgcgc aaaggacgac tgcgcatcat tggctggttg ctggagtgca
      241 gtttcaagca tcgcgagatc gtatttgccc ggttgcgtcc tgatgatgtt cggcaatacc
      301 ttgtccggcg actggctgag aatccgtcgg cctccaatgg ctcacagcat gcagcggcac
      361 tacggagtta cctgcgttac cgagccacct gcggcgatca ggttggcaag ctcgtagcag
      421 tttgtgaacc cagtccattg gcggctcgca accctgccga gggcattgaa cgccgaggat
      481 gctgatcgat tgctgggcac agctgccttg gcacgcaaga cgcccaagcg cgcttatgcg
      541 attgtgcgtt tggcactgga tttggggcta cgctctggtg agattgccca tctcaacctc
      601 aacgatatcg actggcaggc cggcacgctc atgctgcgcg gtaccaagtc gcttcggcag
      661 gatgcggttc ctcttccgga agaaaccggc aaggccctgg ttgactatct ccggcacgaa
      721 cgtcctgcga tccatcaccc ggcggtcttc gttcggcagg tcgccgaccg gatgcttccc
      781 atcacatctt gtgcggtaca gaaggtgatc aagtgtgcgt gccgtcgaat cggtttgcca
      841 gatgccagcg cccatgcttt gcgccatacc gtggcttgcc gtctcgtcga gcgtggcagc
      901 tcgcttaagg aagtagcgga cttgctgcgc caccggtcgc tgaacagcac cctgatctac
      961 gccaagcttg atacgccgaa gttggcggcc gtcgccttgc cttggccggg gagtgcggca
     1021 tgagcgcgca catcagcttg cagcagcgga tcgatgacta tctcgctgaa cggcagcgtc
     1081 ttggtttcaa gctacattcg cgaagtacgc tattggctgg gtttgcccgc tatgttgccg
     1141 atcgcaagca tcgtggcacc ctgacaaccg acctcatgat cgattgggcc aagcaggata
     1201 agtggcaacg cggcacacca gcgacctggg ctgcccggct tgcccgcatt cggcatttcg
     1261 cgcgctacct caagcaattc gaaccggata ccgaagtacc ggaggagttg accttcggcc
     1321 cagaaccggg acgcgtcgcc ccgcacatct tccacgagcc ggaagtcatc gacttgctgg
     1381 cagcggcccg ccagcttgga ccgactggca gcattcgccc cgctacctac gaaaccctgt
     1441 tcggcctgat ggcctccacc ggtctgcggg tttcggaagc gctccacctg cgtgacgctg
     1501 acgtcgatct caaacgtggg atgctgacga ttcggcagac caagttcgcc aagtcgcgac
     1561 tgctgccggt ccacccgacg acggttgcgg cattggcgaa ataccgcaag gcacgcgcag
     1621 cgcatatggc cacggttgcg gacatgccgt ttctgatcag cagtcgcggc cgacggcttg
     1681 ggaagccgct gggagatcgc caggcccacc gcgtcttcaa catgttgcgc gaccatctcg
     1741 gctgggcgaa ccgtggcggt catgccgcca tacgactcca tgacctgcgc catacttttg
     1801 ctgttcgtcg tctgatgcgt tggcatgccg atggcaccga catcgatcag atgatgctcg
     1861 ccctgtcgac ctacatgggc catgccgaga tcttctacac ctattggtat ctcaccgctg
     1921 ttcccgagtt gatggcactg gccggcagca aattcgagca cttcgctgac ctgattggag
     1981 gctgcgatga ataaacatcc gaaattgcca ccgtcgtttc ctgccttggt gcaagcctac
     2041 ttcggcgagt atctgactca gcagcgggcg ctcagtccgc agaccattgc agcctatcgc
     2101 gacggcttcg tgctgttcct taaatttgct gaggcacgat tgggcaaggc gccggctgcg
     2161 atggcgctgg ccgacgttac gccggaactc atcatggcct tcctcgatca tcttgaacga
     2221 cagcgacaca attccgtgcg cagtcgcaac gcccgtttgg ctgcgctacg gtcgtttctc
     2281 aagttcgccg gccaccggga tatagcatcg ctccaggtga tcgagcgtgc cttgggagtt
     2341 cctgtaaagc gtttcgagcg tccgatgttc ggctacctga cccgcgagga ggtactggca
     2401 gtgatcggag aacccggtga ttcatggctg agtcagcgtg atcatgcctt gttcctgttg
     2461 atgtacaaca ccggcgcgcg cgtatcggag atcatcgggg tcaaagttgg cgatgtggtg
     2521 ctcgacgcca gcgccgcttg tgtccatctg cacggcaagg gtcgcaagca gcgcagcgta
     2581 ccgttgtggc aggcgaccgt caaagcacta cgtgcctggt tgcgtgcaaa tctcgaactt
     2641 acggcagagg caaccttgct acccaatcgg aatgggcgcg aaatgacccg cgccaatgtc
     2701 acgctcaggt tggcattgtc ggtccagcga gcaatggaaa ctcagccggc gttggcaaag
     2761 cggaaggttt cgccgcatac cgttcgacat acgacggcca tgcacctgtt gctggcgggt
     2821 gtggatatca gcgtcatcgc actctggctc ggtcatgaga gcccagtgac cacgcaccac
     2881 tacgttgaag ccgacctggc aatgaaggaa cgtgccctgg cccgacttca cgaaccgggc
     2941 gccaagatcc agcgctatcg ggctcctgac tcgctgatca acttcttgcg aaccctgtga
     3001 ttatgaaaag cacggaatgc gtgccatcgc caatcgacca gaccttggcg cgcgtttcaa
     3061 caagctactt tccataatca acgactatgc ataatcggcc aaagccgacc ctgggcctgc
     3121 ggccagaatc gggcggctat gcaccagtta cccgccactc ggccgatgat gtaggtcaac
     3181 ggccgctatc ttgatcgacc taggacggca cccggtagag taggaatgtg gcgcggtagc
     3241 agtaggttcg gtttgtctgt tggc
//
LOCUS       DASRKK010000713         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1344202, whole genome shotgun sequence.
ACCESSION   DASRKK010000713 DASRKK010000000
VERSION     DASRKK010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344202"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFC15_17230"
     CDS             <1..477
                     /locus_tag="VFC15_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM11954.1"
                     /translation="AIEREIRGRPPDQRQQVRQARAKPLLESLRQWLEETLSKLSRKS
                     DTTSAIRYALSRWEALVRYGDDGRIEIDNNAAERSLRAVALGRKNYLFAGSDAGGEHA
                     AAIYSLIGSAKLNGLDPEAYLREVLTRIADHPITHIEELLPWQIAASLPPAIEPAA"
     gene            429..752
                     /locus_tag="VFC15_17235"
     CDS             429..752
                     /locus_tag="VFC15_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11955.1"
                     /translation="MADRRKPTTRNRTGSVDKHAAGKKSAVTSLSLPNIAELIADGEI
                     TVGVLRPIGCVATATDGHNCLAMLVRRRGETLAQLLTRLDLAIAKALTEDIFTDEINP
                     PSKNS"
     gene            1143..3509
                     /locus_tag="VFC15_17240"
     CDS             1143..3509
                     /locus_tag="VFC15_17240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM11956.1"
                     /translation="MSQHRGPLLLILFAVILVSGGYASPIHKSAINRDFAFHSAYAIH
                     SSTSTDDWLGGTGNWSNPADWTGGLPGTGSDVYIDSGGNDLVYLDTSASIASLTLGGD
                     SGTSQLVDNGTPQTLTIAGALTVYQTGALYLAAGNTVTAGADSSNAGFIQLENGSALQ
                     VNGNLSNSGTIYLGYLVGSAGNLTVTGELDNTGVLQLGRRDAFSSGSAVNLGSLVNAG
                     SISVGPSTTLRVDGAVNNTGGIALGFQDGELGSQAHFGSLVNSGAVEIGLGSALTVAG
                     DVNNTATGWVGMDGMAQLLIGGSLTNSGSLITGRNWGGDEVHIGGGLTNSGQIALDGS
                     AQFYVAGVANNPGSLLFDRNFGSGAHAAFGGLVNSGSIEMNNGSGFAVSGIANNSGSI
                     SALWGDEHIPSTFSAFYLTNSGSIYSNFEVGWLNNSGTILLQHFNGYGAVHGNLTNSG
                     DIALADGGYLIVGRDVINSGHITTDYGVNSINIAGRLINTSTGSVVFNGGPEVINLGN
                     VVNQGTLVFGGGNDYNVTGGPHAGTSALSGFINSGIVSIGPDANLNVVGNYTQTAGQT
                     NFLDTGTLRVQGRGMAVFGGGSVYSEFGTISGPVFSNAAISLYQLSILGNYTQGPNGS
                     LTFDIAGTAPGEYDQLNISGHAQFNGLMTVDLLHGFVPDLGDAFDIVHFASESGTFSM
                     VLGLPINGEEHFVLEYNATDLTLDVVSGALLGADNDQATLLASNIASSIAADGGGSGT
                     ASGFTSSTGSHFTSDYGRSSPTPEPGTLLLLGSGLLCAGYTVRRRMTK"
     gene            3596..4012
                     /locus_tag="VFC15_17245"
     CDS             3596..4012
                     /locus_tag="VFC15_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11957.1"
                     /translation="MTLPATPETGTSKRRTFWILGCLVCLLIGLFIGHAATYHRMRAA
                     TSFATPYQAVLLTNGSVYYGKLSGYGGGHPVLTDVYYILSKTDPETKQVTNVLVKRGK
                     ELHGPDRMYLNAGQIIFVEPVGPNSKVAQLINEANQ"
     gene            complement(4157..>4361)
                     /locus_tag="VFC15_17250"
     CDS             complement(4157..>4361)
                     /locus_tag="VFC15_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11958.1"
                     /translation="LDALVKGKYTGPRTKILSAYLTKVARLGGYLARANDPPPGNMVM
                     WRGLSRLTDIELGATIGAQLVGN"
BASE COUNT          935 a         1272 c         1253 g          901 t
ORIGIN      
        1 gccatcgaac gagagatccg agggcgcccg ccggaccagc ggcagcaagt ccgccaggca
       61 cgggccaaac cgctgctcga atctctgcgg cagtggttgg aagaaacttt gagcaagctg
      121 tcgcggaaat ccgacaccac ctcggcgatt cgctacgcgc tctcgcgctg ggaggcgctg
      181 gtgcgctatg gcgatgacgg acgcattgag atcgacaaca acgccgccga acgcagcttg
      241 cgtgccgtgg ctctgggccg gaagaactat ctctttgcgg gatcagacgc cggcggcgag
      301 cacgccgcgg ccatctacag cttgatcggc tcggcgaaac tcaacggact tgatccggaa
      361 gcctacctgc gagaggtact gacgcgcatc gccgaccacc ccatcactca catcgaggag
      421 ttgctgcctt ggcagatcgc cgcaagccta ccacccgcaa tcgaaccggc agcgtagaca
      481 agcatgcagc cgggaagaaa tccgccgtca cttccttgtc cctgcccaac atcgcggagc
      541 tgatcgcaga cggcgaaatc accgttggcg tgctccgtcc tattggttgt gtcgccaccg
      601 ccaccgacgg acataattgc ctggccatgc tggtccgccg ccggggcgag accttggccc
      661 agttgctgac tcggctcgac ctcgccattg ccaaagccct caccgaagac atcttcacgg
      721 acgagatcaa cccgccatcg aagaattcct gaagtgtcca catcactcta ctctttcgtg
      781 gacatccagg cgcaatctcg agagtcgctg ctaatcggct gcgaaggaat ctcttctatg
      841 tgaagcgacc gcccagaaaa gtcaccagtg ccgtgcacat ccggttccaa ttcgggaacc
      901 tctggacgaa aatgggaaag agattcccat tcttgtcttg acttgccgca gcttcgtctt
      961 acagttacct tgagaaaagt gcacgcttcg ataatcgttc tggattatcg cgtgccctcg
     1021 tgagcgggca aagctacgac aggaatgatc gcctgtcgcc ggctcaccgc ccccagttgt
     1081 cgagccccct atcgactcca actttgccta accctcgatc atcaagcgaa gaaggtcccc
     1141 ctatgagcca gcaccgtggg ccactgctgc tgatcctctt tgccgtcatc cttgtaagcg
     1201 gcggctatgc ttctccaatt cataaatctg caattaaccg cgacttcgcg tttcacagcg
     1261 catacgcaat ccacagttcg acttcaaccg acgattggct tggtggcacg ggtaactgga
     1321 gcaacccagc ggattggacc ggcggcctgc ctggtactgg tagcgatgtg tacatcgatt
     1381 ccgggggcaa cgatcttgta tatctggaca cgagcgcgag cattgcctcg ctcactctgg
     1441 gcggcgacag cggcacttca caacttgtcg ataacggcac cccacaaacg ctgaccattg
     1501 cgggagcgct taccgtatac caaacgggcg ccctgtacct cgccgcgggc aacacagtga
     1561 cggcaggcgc cgactccagc aatgccgggt ttattcaact ggagaacggc agcgcgcttc
     1621 aggtcaatgg caacctcagc aactcaggga cgatttactt ggggtatctt gtcggctccg
     1681 caggcaacct gaccgtgacg ggcgagctcg acaacaccgg tgtgttgcaa ctgggtagga
     1741 gggacgcttt ttccagcggc tccgccgtca atctgggcag cctggtgaac gccgggagca
     1801 tcagtgtggg gccgtcgaca acgctccgag tggatggtgc ggtgaacaac acaggaggta
     1861 ttgcgctggg ctttcaggat ggggagctag gcagccaggc gcatttcggg tcgctggtca
     1921 acagcggagc tgtcgagatc ggcttgggct cggcactcac ggtcgccggc gatgtgaaca
     1981 acacggccac cggttgggtc ggcatggacg gtatggccca actcttgatc ggcggttcgt
     2041 tgaccaacag cggttccttg atcaccggta ggaattgggg gggcgacgag gttcacattg
     2101 gtggcggcct cacgaacagc ggccagatcg cgctggacgg tagtgcgcaa ttctatgtag
     2161 ctggcgtagc caacaacccg gggagcctgt tgtttgatcg caattttgga agcggagccc
     2221 atgcagcgtt cggaggcctg gtcaacagcg ggtccatcga aatgaacaat ggatctggct
     2281 tcgctgtctc tggcatcgct aacaacagcg ggtccatttc ggccctatgg ggggatgaac
     2341 acataccaag cactttcagt gccttctacc tgacgaatag cggatcgatt tactccaact
     2401 ttgaagttgg atggctgaac aacagcggca ccatcctgct gcagcacttc aacggctatg
     2461 gcgcggttca tgggaacctg accaacagcg gagacattgc cctcgccgat ggaggctacc
     2521 tgattgttgg gcgtgacgtg attaactctg gccatatcac gactgactac ggcgtaaata
     2581 gcatcaacat tgccggcagg ctgatcaata cctcaacagg aagcgtggtt ttcaatggtg
     2641 gtcccgaggt catcaacctg gggaacgtag tgaaccaggg aacattagtg ttcggcggcg
     2701 gcaacgacta taacgtaact ggcgggccgc acgcgggcac ctcagcgctc tccggcttca
     2761 tcaacagtgg aattgtgagc atcgggccgg atgccaatct caacgttgtg ggcaactaca
     2821 cccagaccgc aggacagacg aacttccttg atacgggtac cctacgggtc cagggtcggg
     2881 ggatggccgt tttcggaggc ggttccgttt acagcgagtt cggtacaatc agcgggccag
     2941 tttttagcaa tgctgccatc tccttgtacc agctttccat ccttggcaac tacacccagg
     3001 gaccgaatgg aagcttgacc ttcgatattg cgggaacggc gcccggagag tacgaccagc
     3061 tcaacatcag cggtcacgcc cagttcaatg gcctgatgac ggtggacttg cttcacggct
     3121 ttgtgcccga cctgggcgat gccttcgaca tcgtgcactt tgcgagcgag tcgggaacgt
     3181 tctcgatggt cttgggcttg ccgatcaacg gcgaggaaca ctttgtgctg gaatacaacg
     3241 cgaccgattt gacgctcgac gtggtgtcgg gcgcgctgtt gggagccgac aacgaccaag
     3301 cgactctcct cgcatccaac attgcatcca gcatcgcagc tgatggtggt ggatctggta
     3361 ctgcatctgg cttcacctcg agcaccgggt ctcactttac gtcggactat ggccggtcct
     3421 cgccaacacc ggaaccaggc accctattgc ttcttggttc gggcttattg tgcgcgggct
     3481 atactgttcg gcgccgcatg actaagtaac aggacggcat tggcccatag gggggccagt
     3541 gccgtcttat ctcccgccag gcccggcatc tgagccagaa aatcaggaga agagtatgac
     3601 cctaccagca acacctgaaa ccggaaccag caagcgacga acattttgga ttctgggatg
     3661 cctggtctgc ctgctgattg gtctgttcat cgggcacgct gcgacctacc atagaatgcg
     3721 cgccgccacc agctttgcga caccttatca ggcggtattg ctgacgaatg gttccgtgta
     3781 ttacggcaag ctgtcgggct atggaggcgg gcatcccgtg ctgaccgacg tttactacat
     3841 ccttagcaaa acggatccgg aaaccaagca ggtcaccaac gttttggtga agcgtggtaa
     3901 ggaactgcat ggtcctgacc gcatgtactt gaatgcgggc cagattatct ttgtggaacc
     3961 agtgggacca aattcaaagg tcgcccaact gatcaatgag gccaatcagt aaagttcccc
     4021 acgcaaagaa gatggccgtt gactgcaatg ttaaagacgg ccacctccag ccttttcgag
     4081 gacgccgcct cttcggaaga tgttgttaca ggctctgagt atttatatgc cttgtcaaca
     4141 cggcctactc cggctttcaa ttacccacaa gttgagcacc gatggtggcc cccagttcga
     4201 tatccgtaag acgggacaag cctctccaca tcaccatgtt gccgggtgga ggatcgttcg
     4261 ctctggcgag gtagccgcca agccgcgcaa ccttggtgag ataggcggag agtatcttgg
     4321 tgcgcgggcc ggtatatttg cctttcacca gcgcgtccag c
//
LOCUS       DASRKK010000714        11065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_659925, whole genome shotgun sequence.
ACCESSION   DASRKK010000714 DASRKK010000000
VERSION     DASRKK010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659925"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1118)
                     /locus_tag="VFC15_17255"
     CDS             complement(234..1118)
                     /locus_tag="VFC15_17255"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZM11959.1"
                     /translation="MPATKRSLLPLAVALLAFVIPALSQTKLAPKWEELTASDFVQAI
                     HQSQGVCLLPIGIIEKHGPHLPLGTDLLDVRFAVMNAVQQEYAVVFPEYYFGQIFEAQ
                     QQPGTVAYSLSTQITLLQETVKEMARNGCQKILIVNGHGGNNSLLPLFAQAQLATPRN
                     YVVYVFGLPREDVPGRPPLKTKFDMHGGEVETSSMMIARPDLVHVDRADSESGADQNR
                     VKLPESVYTGIWWYAKFPDHYAGVGSAANTTLGEFDQKEWSRQIVEAIKAVKADQESL
                     KLQNEFFEKTQHPLDTKQ"
     gene            complement(1187..1996)
                     /gene="lipB"
                     /locus_tag="VFC15_17260"
     CDS             complement(1187..1996)
                     /gene="lipB"
                     /locus_tag="VFC15_17260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZM11960.1"
                     /translation="MVTNVLFLGRVAYGTALQLQRTLLEMRKAGRIENTVLLLEHPPV
                     ITLGRNARLANVLAPPEFLAQQGVELFDIDRGGDVTFHGPGQLIAYPIVDLRSFEPKV
                     GAVEYVRRLEEVLIRTCSDFGVGTQRIKGLTGVWTYALPDKPEAKIAAIGVHISRSVT
                     THGFALNVSTDLDSFSLIVPCGITNKPVTSLERESQKTLPLEDVATSASRNFGRVFQT
                     QMLCLESLEDLLAQGAVPTPKRVSDGPANQDTPARPLKELRDLLGDGNELA"
     gene            2166..4418
                     /locus_tag="VFC15_17265"
     CDS             2166..4418
                     /locus_tag="VFC15_17265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZM11961.1"
                     /translation="MKRVVEEPAAIREPATDARAGHKYVSNFIFSLLILAVAAAGAIS
                     GLLLVYSTDLPQVTELEKYRPSTITELYDDQNRVIGQFALQRRVIDKYDDFPAVLRDA
                     LISTEDKDFERHWGVDFWRVFGAAYRDVAVGGRAQGASTLTMQLSRNLFLSPDRNFRR
                     KMQEVMLAIQIERRFTKQQIFTLYANQIYLGHGVYGFEAGSNFYFGKKAKDLTLEEAA
                     VLAGLPKAPNAYSPINNPERALHRRNLVINNMLEDGKITAEEAQRAKNSPIRLNVQGE
                     PNSIAPYFVEELRQYLEKKYGSEEVHEKGLRVYTSLNVEMQKAANLAVLDGLAAYEHR
                     HGWKGSLLNVVANGESLPTYKHVDWDGDLSAGNFVHALVTAVGPQYATVKFGPYQAEL
                     GPAEIKWTHYNSPQQFLTRGDLVYIKILELNGTQSRVSLEQESGVQGALLALDNSTGD
                     VKAMVGGRSFDESKFNRSTQALRQVGSSFKPYIYTAAIDQGMTPDDTVLDAPVSFPGG
                     PNGPWVPHNYDGKFEGVITFRRALAHSRNIPALKVTERVGGIKTVIEYARKFGITAPL
                     LPYLPVALGAADITLWEQTAAFTTFPNDGVRVAPRMIRKVEDYDGRVLEENYPEVKDV
                     VSARTARIMVDMLREVVLHGTGAAAAKLNHPLGGKTGTTNDFTDAWFVGFSPSLTCGV
                     WVGFDEKKTLGNKETGAVAALPIWMDFMRVALQGRESEDFAAPPPAPPNAVAHKVDTD
                     DQTPGDGETH"
     gene            4418..5158
                     /locus_tag="VFC15_17270"
     CDS             4418..5158
                     /locus_tag="VFC15_17270"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZM11962.1"
                     /translation="MRRFFRWLVRALVLIVVFMASALTAMRFAIHGRQTHVPKIVGMT
                     PHDAENALESSGLVFEPADRFFSAEVPAGRILSQVPSPGEEVRRGWHVRVAESMGPQR
                     VTIPDLSGDSERSAEINIRRRGLEMGTMAVASIPDTTPDQIVAQSPPANAVNVSAPKV
                     SMLAAAAEDRKSYVMPDLRGRTEDEAVNAIVGASMKVGSITSQPIAEGEASSPETLPA
                     GTRMVVKTIPGAGQRVYEGQSVNLEVTR"
     gene            complement(5198..6532)
                     /gene="rsmB"
                     /locus_tag="VFC15_17275"
     CDS             complement(5198..6532)
                     /gene="rsmB"
                     /locus_tag="VFC15_17275"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZM11963.1"
                     /translation="MPVSPARAAAFDILLRVEQQDAYASELLHSERLDTLSLADRGLT
                     TEIVMGVLRWRSRLDDAIAAQSSRPLGKLDPQVLTALRIAAYQLLYLSRVPARAAINE
                     SVDLVKRAHKVSAAPFANAVLRKLANTKPETVAANTSATTAALLAHEFAHPEWLVERW
                     VDHLGIDNVEGVCRHDQRIPTTSIRLDGAAVEGELKNEGIELAPGTLLTAARIVTSGD
                     VTQTRAYREGRVFIQDEASQLVAALVGTGSRLLDCCAAPGSKTAAIASRNPAAEIVAV
                     ELHPHRAELLRRRVRATNVQVITADTLHIPLKGGFDGVLADVPCSGTGTLARNPEIKW
                     RLKPADLGELHSKQVAILRAALQQLTPGGRAVYSTCSLESEENQAVVEEVLQGDSKFT
                     LLNCRTELERLRAEGELAISDLDSLLEGKYLRTLPGVHPCDGFFAAIIQRLN"
     gene            complement(6538..7479)
                     /gene="fmt"
                     /locus_tag="VFC15_17280"
     CDS             complement(6538..7479)
                     /gene="fmt"
                     /locus_tag="VFC15_17280"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZM11964.1"
                     /translation="MDLVFCGTPMFAVPTLEKLVDAGFSVKLVVTQPDRASGRGMELA
                     ASPVKLSALERGLPILQPDKIKSNDDFRAILESLQPEAIIVVGYGRIIPQWMIDLPQL
                     GNLNLHASLLPKYRGAGPIQWAIANGESITGVTTMRIDAGLDTGDILLQTEEPIRPED
                     TALTLSPRLAHGGAELMINTLAGLANGTIKPQPQDNSKATLAPILKREDGLIGFSRTA
                     TETWNCLRGFQPWPGAFTTFRGKTLHLHAAVPAPEVALVQAGHFVVEKDRLLLGFARG
                     STLEVLELQMEGKKRMSARDFVNGYRPRSDEPLGATG"
     gene            complement(7490..7999)
                     /gene="def"
                     /locus_tag="VFC15_17285"
     CDS             complement(7490..7999)
                     /gene="def"
                     /locus_tag="VFC15_17285"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZM11965.1"
                     /translation="MIYPIVKYGDAVLEKPSAPITKFDDELRKLADDMFESMYAAHGV
                     GLAAPQIGISKRIAVIDVTFKEDPEAKIVLVNPEIVRVEGRITSNEGCLSLPEFREKV
                     TRPRKVTARAQNLRGETFEVTGEELLARALLHETDHLNGKLYISHISALKRDLMKRKI
                     RKLVKLGEW"
     gene            complement(8000..9178)
                     /gene="aroC"
                     /locus_tag="VFC15_17290"
     CDS             complement(8000..9178)
                     /gene="aroC"
                     /locus_tag="VFC15_17290"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZM11966.1"
                     /translation="MLRFSTAGESHGEALVALLSGMPAGLAVDLEFVNRELWRRQQGY
                     GRGGRMRIERDTAHILSGVRHGHTIGSPIAMVLANNDWQNWTEALPVETGDPTKHKRV
                     GSPRPGHADLAGAVKYNFSEARYVLERASARESAARVAAGALAKLLLRELGIEVLSHV
                     IAVGSAKLDAPVTWQAIQAVVAKEDILLSCIDPDSEIRMKEQVDLALRTGDSVGGIFE
                     VVAHNVPPGLGTYANWDERLDTHLAAAVMSLQAVKGVEIGDAVEAATALGSAVHDEIG
                     YASGGGGTFTGFTRSSNHAGGLEGGVSNGEDIRIRGYLKPISTLRRPLGSVDFASREP
                     VKAAYERSDVCVVPAAGVAAEAMVALTLARFALEKFGGDSVLELRRNFEGYCQQLRNF
                     "
     gene            complement(9243..9674)
                     /locus_tag="VFC15_17295"
     CDS             complement(9243..9674)
                     /locus_tag="VFC15_17295"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZM11967.1"
                     /translation="MLKFRQSDFDEIRRHGEDTYPHECCGVLLGVMDGDTREVRATVR
                     CGNTRIDSPQNRYNIDPRELVRVQREARGRELDIVGFYHSHPDHPARWSPTDFREAHW
                     IGCSYVITSVEKGNAVVTNSFALTGTSEEDKALVDEVIVVE"
     gene            complement(9720..10676)
                     /locus_tag="VFC15_17300"
     CDS             complement(9720..10676)
                     /locus_tag="VFC15_17300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZM11968.1"
                     /translation="MNRTSAIPATGLSIIERIGNTPLLRFESISAELPHAEIYAKAEW
                     TNPGGSVKDRAAAAIVEEAFRSGKMNGGKRLLDSTSGNTGIAYAMLGAACGFGVTLCM
                     PSNVSLERKRILNAYGAEIIFTAAGEGSDGAILKAREIFAEQPDKYFYADQYSNDANW
                     QAHYCCTAKEIWEQTRGRITHFVTMLGTSGTFMGTTRRLKELNLRIECISLQPDSPFH
                     GIEGAKYMPASIVPRIYDPALADRNLEISTEDCYAMVRRLARDEGLMVGPSSAASLVG
                     ALKVARDSKVDHPVVVCIFCDGADKYLSERFWDDGPHPDYEI"
BASE COUNT         2221 a         3232 c         3387 g         2225 t
ORIGIN      
        1 gacataatct cgtcgcagtt aatacgacat tttctcgttg caactacagc ctagagcgat
       61 ttgcacttca gcggtcagga aagagtttcg caattacctc aggggctaaa gccgagtgca
      121 aaagacgatg tcagatgcac cgctgaagcg gtgttccacc caacatcgca attgtgaaac
      181 tgttgaatgg gcggccgaaa tcggcagacg cccaagcgat gcaggacgac tgctcactgc
      241 ttcgtgtcca gcggatgctg cgtcttttcg aagaactcgt tctgcagctt caagctctcc
      301 tgatcggcct ttactgcctt gatggcctca acgatctggc gcgaccactc cttctgatcg
      361 aactcgccca gcgttgtgtt ggcggccgaa ccgacgcccg cgtagtgatc gggaaacttg
      421 gcgtaccacc agatgccggt gtaaacggat tcaggcaact tcacgcggtt ctggtcggcg
      481 ccggattcgc tgtcggcgcg atcaacatgt acgaggtccg gacgcgcgat catcatggac
      541 gacgtctcaa cctccccacc atgcatgtcg aacttggtct tcagcggcgg gcggccgggg
      601 acgtcctcgc gcggaagccc gaagacgtac accacgtaat tgcgcggagt ggctagttga
      661 gcctgcgcga acagcggcag caggctgttg ttgccgccgt gaccgttgac gatcaggatc
      721 ttctggcaac cgttgcgcgc catctccttc accgtttcct gcaggagcgt gatctgcgtg
      781 ctcaggctgt aggcaacggt tcccggctgc tgctgcgctt cgaagatctg tccgaagtag
      841 tactcgggga acacgaccgc atattcctgc tggacggcgt tcatcaccgc gaagcgaacg
      901 tcgagcaaat cggtaccgag cggaagatgc ggaccatgct tttcgatgat gccgatggga
      961 agcaggcaga caccttgcga ctgatggatg gcctgcacga agtcggaagc tgtgagctct
     1021 tcccacttgg gggcgagctt ggtctgcgac agggcgggaa tcacgaacgc gaggagagcg
     1081 acagcaagag gtagaaggga ccgcttggtg gctggcatga aacctccaga atcatgaaac
     1141 aggcgattct acccgaaccc gggtggaggc gcggagcttt tgccggttat gccagctcgt
     1201 tgccatcgcc cagcagatcg cgcagctctt tcagagggcg cgcgggcgtg tcctgattgg
     1261 cggggccgtc actcactcgc tttggggtgg ggacagcgcc ttgcgcaagc aggtcttcca
     1321 gcgactccag acaaagcatc tgggtttgaa acactctgcc gaagtttctg gacgcggagg
     1381 ttgccacgtc ctccagagga agcgtttttt gcgactctcg ttccagcgac gtgacaggtt
     1441 tgttcgtaat gccacacgga acgatgagag agaacgagtc gagatcggtg gacacgttca
     1501 aagcgaagcc atgagtcgtg actgagcgcg aaatgtgaac gccgatggcg gcaatcttgg
     1561 cctccggctt gtcgggaagc gcgtaggtcc agacgcccgt cagccccttg atgcgctggg
     1621 tgccaactcc gaagtcgcta caggtgcgga tcagaacttc ttctaggcgg cggacgtatt
     1681 ccacggcgcc aaccttaggc tcgaagctgc gcaggtcgac gatcgggtag gcgatcagtt
     1741 ggccggggcc gtggaaggtc acatcgcctc cgcggtcgat atcgaacagc tcaacccctt
     1801 gctgggcgag gaactccggc ggcgccagaa cattggccag gcgggcattg cgtcccaagg
     1861 tgatgaccgg agggtgctcc agtagaagca cggtgttttc gatgcggcca gccttgcgca
     1921 tctccagcag cgtgcgctgc agttgcaacg ctgtgccgta ggcgacgcgg cccagaaata
     1981 gaacgttagt caccatagaa agctaagcct caggctatca gccgagtgcg gtagtacctc
     2041 gatgaatctt cgacagtacc attcctcttg gatgatgggg aactccgatc ggtggcacgg
     2101 agcgccagct gggtaaccgg tcgggggtct tttctggtgc tatcctgaat ccagtcccac
     2161 cccccatgaa acgcgttgtc gaagagcctg ccgcgatccg cgaacctgca accgatgcta
     2221 gagctgggca caagtacgtc agcaatttca tattctcgct attgattctg gcggtcgcag
     2281 cggcgggagc gatctctgga ctgctgctcg tgtattccac cgaccttccg caggtaaccg
     2341 agctggaaaa ataccggccc agcaccatca ccgagctata cgacgaccag aaccgcgtga
     2401 tcggacagtt tgcgctgcag cgccgggtga tcgacaaata tgacgacttc cctgccgtgc
     2461 tgcgcgacgc gctcatctcg actgaggaca aggatttcga gcggcactgg ggcgtggact
     2521 tctggcgcgt tttcggcgcg gcctaccggg atgtcgcggt tggtggccga gcgcagggcg
     2581 cttccacgct caccatgcaa ttgtcgcgca acctgttcct ctcgcccgac cgcaacttcc
     2641 ggcgcaaaat gcaggaggtg atgctggcca tccagatcga gcggcgcttc accaagcagc
     2701 agatcttcac gctgtatgcc aaccagattt acctgggcca cggcgtgtat ggcttcgagg
     2761 caggatcaaa tttttacttt ggaaagaaag ccaaggacct gaccctggaa gaagccgcgg
     2821 tgctcgcggg cctgcccaag gccccgaacg catattcgcc catcaacaat cccgagcgcg
     2881 cactgcaccg tcgcaacctg gtgatcaaca acatgctgga agacggcaag atcaccgccg
     2941 aggaggcgca gcgcgccaag aattccccga tccggctcaa tgtgcaaggg gagcccaaca
     3001 gcatcgcgcc ctactttgtg gaggagttgc gccagtatct ggagaagaag tatggcagcg
     3061 aagaggtgca tgaaaaagga ctgcgtgtct atacgtctct gaacgtcgag atgcaaaagg
     3121 ccgccaatct agcggtgctc gacgggttgg cggcttatga gcaccgccac ggatggaagg
     3181 gcagcctgct gaatgttgtc gccaacggcg agtcgctgcc aacctacaaa cacgtcgact
     3241 gggatggcga tctgtcggcg ggaaattttg ttcatgccct ggtcactgcc gtcggacccc
     3301 agtacgccac cgtcaagttc gggccttatc aagcggagct tgggccggct gaaatcaagt
     3361 ggacacacta caactctccg cagcagtttc tgacgcgcgg cgacttggtg tacataaaga
     3421 tcctggagtt gaatggcacg cagagccggg tttccctcga acaggagtca ggtgtgcagg
     3481 gagccttgct ggcgctggat aattccaccg gcgacgtgaa ggccatggtg ggcggacgca
     3541 gcttcgatga gtcgaagttt aaccgctcga cccaggcgct gcgccaggta ggatcgtcgt
     3601 tcaagccata tatctacacg gccgcaatcg atcaagggat gacgccggat gacacggtgc
     3661 tcgacgcgcc ggtcagcttt ccgggcggac caaacggacc ctgggtcccc cacaattacg
     3721 atggcaagtt tgaaggcgtg atcacgtttc gccgggcgct ggcccattcg cgcaacattc
     3781 ctgcgttgaa agttaccgag cgcgtgggcg gcattaagac cgtcatcgaa tacgcgcgca
     3841 agtttggcat cactgcgcca ttgctgccgt atctgccagt ggcactaggc gcggctgaca
     3901 ttacgctttg ggaacagacc gccgcgttca cgacttttcc caacgacggc gttcgggtgg
     3961 cgccgcgcat gattcgcaag gtcgaggact acgacggccg cgtattggag gaaaactatc
     4021 ctgaggtgaa ggacgtggtc agcgcgcgca ccgcccgcat catggtcgac atgttgcgcg
     4081 aagtggtact gcacggcacc ggagcggccg ccgcgaaact caaccatccg ctgggaggca
     4141 agacgggcac gaccaacgat ttcaccgacg cttggttcgt cgggttctcg ccgtcgctga
     4201 cttgcggcgt gtgggtcggt ttcgacgaga agaagacgct gggcaacaaa gaaacgggcg
     4261 ccgtggctgc cttgcccatt tggatggact ttatgcgagt cgcgttgcag ggtcgcgaat
     4321 cggaagactt cgccgcaccg cctccggcgc cgcccaacgc cgttgctcac aaagtggata
     4381 ctgacgatca aacgcctggc gatggggaga cgcactgatg cgccgcttct ttcgctggct
     4441 ggtacgcgca ttggtgctga tcgtggtctt catggcctcg gccctcacgg ccatgcgctt
     4501 cgcaattcat gggagacaga cgcacgttcc caagatcgtt ggcatgacac cgcacgatgc
     4561 ggagaacgcc ctggagtcga gcggattggt attcgaaccg gcggaccgct tcttcagtgc
     4621 ggaggttccg gcgggccgga ttctttcgca ggtaccatct cccggcgagg aggtgcggcg
     4681 cggttggcat gtgcgtgtgg ccgaaagcat ggggccgcag cgcgtaacca ttccagacct
     4741 ttcaggagac agtgagcgtt cggctgagat caatattcgt cgccgcggtc tggagatggg
     4801 gacgatggca gtggccagca ttcccgatac gacgcctgac cagattgtgg cgcagagtcc
     4861 gccagccaac gcggtcaacg tctctgcgcc aaaagttagc atgttggcgg ccgcagccga
     4921 ggaccgcaag tcctacgtca tgcccgatct gagaggacgg acggaagacg aggcggtgaa
     4981 cgcgatcgtt ggagccagta tgaaggtagg cagcatcact agtcagccga ttgcggaagg
     5041 agaggccagt tccccggaaa ctctgccggc tggtacgcgc atggtggtga agacgattcc
     5101 tggcgccggg cagcgggtgt atgaagggca gagcgtgaac ctggaagtga cgcggtgagc
     5161 gagtggtaat tagtaagacg taattagtaa ttggtaatta gttaaggcgc tggatgatgg
     5221 cagcgaagaa tccgtcgcac ggatgtacgc ccggaagcgt gcgcagatac tttccttcga
     5281 gcagtgagtc gagatcgctg attgctagtt cgccctctgc gcgaagtctt tcaagctctg
     5341 tgcggcagtt aagcagcgtg aatttcgagt cgccctgcag cacttcttcc accacggcct
     5401 gattctcttc cgactcaagt gaacatgtgg aatagaccgc gcgccctccc ggcgtgagtt
     5461 gttgcagcgc ggcgcgaagg atcgctactt gtttcgagtg gagttcgcct aagtctgccg
     5521 gcttgagtcg ccacttaatt tctgggttgc gggccagcgt gccggtgccg gagcatggaa
     5581 cgtcggcgag gaccccatcg aagccgccct tgagcggtat atgcagggtg tcggccgtga
     5641 tcacctgaac attcgttgcg cgcacccgcc gccgcaacaa ttccgcgcga tggggatgaa
     5701 gctcaaccgc gacaatctcc gccgccggat tgcgtgaggc gattgctgcc gtcttacttc
     5761 ctggcgcggc gcaacaatcc agcaggcgcg atcccgtgcc cactaatgct gcgacaagtt
     5821 gagaagcctc gtcctgaatg aatacgcggc cttcgcggta ggcccgagtc tgcgtgacgt
     5881 caccgctggt gacgatgcga gcagcagtca gcaaagtacc gggtgcgagt tcgatgcctt
     5941 cgttcttgag ttcgccttcc actgccgcgc cgtcgagacg aatggatgtt gtcggaatac
     6001 gctgatcgtg ccggcagacg ccttcgacat tgtcgatgcc gagatgatcg acccagcgtt
     6061 cgaccagcca ctcgggatgg gcgaactcgt gcgctagaag cgcagcagta gtggccgacg
     6121 tatttgccgc gactgtttcg ggcttagtgt tcgccagttt gcgcaagacc gcattagcaa
     6181 acggcgcggc tgacaccttg tgcgctcgct tcaccaggtc cacgctttca ttgatggccg
     6241 cgcgcgcggg cactcgactc aggtacagaa gctgataggc ggcaatgcga agcgctgtaa
     6301 gtacctgggg gtccaacttg cccagcggtc gagaggactg cgcagcgatg gcgtcatcaa
     6361 gccgcgatcg ccagcgcaac acgcccatca cgatttcggt ggtgagtccg cggtcggcga
     6421 ggctgagggt gtcgagcctc tcggaatgca gcagttcgct ggcataggcg tcctgctgct
     6481 ccacccgtaa caggatgtcg aaggcagcgg cgcgtgcggg agagaccggc attagggtta
     6541 ccctgtcgcg cccagcggct cgtcgctgcg cggacgatag ccgttaacga aatcgcgggc
     6601 gctcatccgc ttcttgcctt ccatttgcaa ttcaagaact tcaagcgtcg atccgcgggc
     6661 gaagcctagc agcaggcggt ccttctccac gacgaagtgc ccagcctgca cgagcgcaac
     6721 ctcaggggcc ggcacagccg catgcaggtg cagcgtcttg ccgcgaaatg tggtgaacgc
     6781 tcccggccac ggctgaaatc cgcgcaggca gttccaggtc tcagtcgcag tgcgcgagaa
     6841 accgatcagg ccatcttccc ttttgagaat aggcgcaagt gtcgcttttg agttgtcttg
     6901 cggctggggc ttgatcgtcc cgttggccaa tccggctaat gtgttgatca tcaactcggc
     6961 gccgccatgc gccagcctcg gcgacaacgt cagagctgtg tcctcaggcc ggatgggctc
     7021 ttcagtctgc agcagaatgt cgccggtatc cagtccggca tcgatgcgca tggtggtcac
     7081 gccagtgatc gactcgccgt ttgcgatcgc ccattgtatg gggccggcgc cgcgatattt
     7141 cggcagcaac gacgcgtgca ggttgaggtt tccgagctgt ggcagatcga tcatccattg
     7201 cggaataatc cggccgtagc cgacgacgat gatcgcctcc ggttggaggc tctcgagaat
     7261 tgcgcggaaa tcatcgttgc tcttgatctt gtcgggctga aggatgggca gtccgcgctc
     7321 cagagcggag agtttcacgg gcgacgccgc caattccatg ccgcgtccgc tggcccgatc
     7381 gggctgggtt acgactagct ttacgctgaa tccggcgtcc accagcttct ccaaggtggg
     7441 caccgcgaac atcggcgtgc cgcagaagac caggtccatc acggctgcac taccattcgc
     7501 ccagtttaac gagcttgcgg atcttgcgct tcatcaagtc gcgcttgagc gcgctgatgt
     7561 ggctgatgta aagcttcccg ttgaggtggt cagtctcgtg caacagggcg cgcgccagca
     7621 actcttcgcc cgtgacttca aaggtctcac cccgcaagtt ctgggccctc gcagtgacct
     7681 tgcggggacg agtcactttc tcgcgaaact ccggcaggct caggcagcct tcgttggagg
     7741 tgatccgccc ttcgacgcga acaatctcag gattgaccag cacgatcttg gcctcgggat
     7801 cttccttgaa tgtgacgtcg atgaccgcga tgcgtttgct gatgccgatt tgcggcgccg
     7861 ccaagcccac gccgtgagcg gcatacatgc tttcgaacat gtcgtccgcg agcttgcgta
     7921 gctcgtcgtc gaattttgta atgggggccg aaggtttctc cagtaccgcg tccccgtact
     7981 tgacgattgg atagatcatt tagaagtttc gtaactgttg acaatagcct tcgaaattgc
     8041 ggcgcagttc gagcaccgaa tcgccgccaa acttttccag tgcgaaccgg gccaatgtca
     8101 gagcaaccat ggcttcggca gctacacccg ccgccggaac tacgcacaca tccgagcgct
     8161 cgtaggcggc ttttacgggc tcgcggctgg caaaatccac cgatcccaag ggacgccgca
     8221 gcgtggaaat cggcttcagg tagccgcgga tgcggatgtc ttcgccgtta gacacgccgc
     8281 cttccagtcc accagcatgg ttggagctgc gcgtgaaacc ggtaaacgtg cccccgccgc
     8341 cggacgcata accaatttca tcgtgcaccg cagacccgag tgcggtagcc gcctcgacgg
     8401 cgtcgccaat ctccactccc ttaaccgcct gcagcgacat gaccgccgca gcgaggtgcg
     8461 tatctaaccg ttcgtcccag tttgcgtagg tgccgagacc gggaggaaca ttgtgcgcca
     8521 caacttcaaa aattccgcct accgaatcgc ccgtacgcag cgccaggtcc acctgctcct
     8581 tcatgcgaat ctcggaatca ggatcgatgc agctcagcag aatatcctct ttcgctacca
     8641 cggcctgaat tgcttgccag gtgacaggcg cgtccagctt tgcgcttccg acggcgatca
     8701 cgtggctcag gacttcgatg cccaattcgc gcagcagcaa cttggccagc gctccggcag
     8761 cgacacgggc tgccgactcg cgggcggaag cacgttccag gacatagcgt gcctcgctga
     8821 agttgtactt caccgcgccc gcaaggtcgg catgtccagg ccgcggcgat cctactcgct
     8881 tatgtttggt gggatcgccc gtttcgaccg gaagagcttc agtccagttc tgccagtcgt
     8941 tgttggccag aaccatggcg atgggcgagc cgatagtatg cccatgacgc acgccactca
     9001 ggatgtgcgc ggtgtcgcgc tcgatgcgca tgcggccgcc gcgtccgtag ccttgctggc
     9061 ggcgccagag ttcgcggttg acgaattcga gatcaaccgc cagccccgca ggcataccgg
     9121 agagcagcgc gaccagcgct tctccatgcg attcaccggc tgtagaaaat cgtagcattg
     9181 ggaatttttg attgtagctg atgagggtat gggcaaggcc cgtgcaggcc ctccctgggt
     9241 gatcattcga ctacgattac ctcgtccacc agcgccttgt cttcttcgct ggtgccggtc
     9301 agcgcgaacg agttggtcac cacagcgttg cctttttcga cgctggtaat cacgtaggaa
     9361 cagccaatcc agtgggcttc ccggaagtcg gtcggagacc agcgcgccgg gtggtcgggg
     9421 tgagagtggt agaagcctac gatgtccaac tcacgcccgc gggcctcgcg ctgtactcgc
     9481 accaactcgc ggggatcaat gttgtagcgg ttctgcggcg agtcgatgcg agtgttgcca
     9541 cagcgtaccg tcgctcgcac ttcgcgggtg tcgccatcca tcactcccag caacactcca
     9601 cagcactcgt gcggataggt atcttcccca tggcggcgaa tctcgtcaaa gtcggattgg
     9661 cggaatttca gcatggatcg gaactgttat gtgcgcgtgc gattcaaggt tgccagctgc
     9721 tagatttcgt agtcgggatg cggaccgtcg tcccagaaac gttcgctgag atacttgtca
     9781 gcaccatcac agaatatgca gaccaccact ggatggtcca ccttactgtc gcgtgcaacc
     9841 ttcaaggcgc ctaccagtga agctgcggac gacggtccaa ccatgagtcc ttcgtcgcgg
     9901 gccagccgcc tgaccatggc gtagcagtct tcagtgctga tctcgagatt gcggtcggcc
     9961 aacgcgggat cataaattcg cggcacgatg gaagccggca tgtacttcgc tccttcgatg
    10021 ccgtgaaagg gcgagtcggg ctgcagcgaa atacattcaa tccggagatt gagttctttc
    10081 agccggcgag tggtgcccat gaacgttcca gaggttccga gcatggtcac gaaatgggta
    10141 attcggcccc gggtttgctc ccagatctct ttcgcggtgc agcaatagtg cgcctgccag
    10201 ttggcgtcgt tggaatactg gtcggcgtaa aaatacttat caggctgttc tgcgaagatc
    10261 tcgcgcgcct tgaggatcgc accatcagag ccttcgcccg ccgccgtaaa gataatttcg
    10321 gccccgtatg cattaagaat tcgcttgcgt tccagcgaaa cgttggacgg catgcagagg
    10381 gtcacgccga aaccgcacgc tgcgcccaac atggcataag cgatgccggt atttccgctg
    10441 gtcgaatcca aaaggcgttt gccgccgttc atcttgccgc tacgaaaggc ctcctcgaca
    10501 atggccgcag cagcgcggtc cttcaccgag ccgccgggat tcgtccactc ggccttggcg
    10561 taaatctcgg catgcggcag ttcggcagag atgctttcga agcgcaacag cggcgtgttg
    10621 ccgatgcgct caatgatcga gaggccggtg gccggaatcg ccgaagtcct attcatgagc
    10681 atttcacttg cttgctgttt cgccttgcag tgtgcgacag ttgtggtttc cagacccgag
    10741 cgccgggatg acacgaagcg actgccgacg tgtgggccag taaacgcagc tgcatccctg
    10801 caactttagt cctaataaag taatcaattc gatgcgaagg cgggtaattc gattccgccc
    10861 caagagacag ccacagccgg ctgaaagcag agcaaatggc aaagaaagtc aaagttaaga
    10921 gttacgaaga cgcggtgagc tggtttcgcg atcatgggtt cgacgtgctc gaagccccgg
    10981 gaaccaacgg gcgcgtgttt ctcaagaagt acaacgcctc ggcggccatc gagcgtgcgc
    11041 ccgacggcgg ggtgaagatt ttcgc
//
LOCUS       DASRKK010000715         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_952770, whole genome shotgun sequence.
ACCESSION   DASRKK010000715 DASRKK010000000
VERSION     DASRKK010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952770"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..2037)
                     /locus_tag="VFC15_17305"
     CDS             complement(466..2037)
                     /locus_tag="VFC15_17305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM11969.1"
                     /translation="MDLLPVILSIAQAEGPDSVLGQIIKAIMANEHVALARIWFLDRD
                     GDCPVCGQPGDEMTLHMRASDGRPLEPGADWSQVNRSHHRVSLDSPTKLAQMVRTEEP
                     VLIADIASDTGGAIDRGWAKRECIEGLIGHPLRFRGAIVGVMAVFLRVHPNKNTLDWL
                     NSFSAHAAVAIGNCRAFQQIDKLRRQLELERDYLREEVVETGDFRDIVGHSPALTRVL
                     QQIDLVAQTDSAVLIQGESGTGKELIARAIHQRSRRGHQPLIRVSCGSIPRELFESEF
                     FGHVKGSFTSAIRDRIGRFQLADGGTLFLDEVGEIPLDLQSKLLRVLQEGEFERVGDD
                     HTRRVNVRVIAATNRDLRTESEAGRFRLDLFYRLSVFPLDLPPLRERREDIPQLAAHL
                     VKLACVRMHAPEPRLPRRELDRLQQYDWPGNVRELQHVIERAVILSRGAPLEFQLQDR
                     PSANLATVVSNHSADFLTEAQWRSLERENLLKALKQAGGKVSGAGGAAELLGVNPNTL
                     ASRLRTLGILRSHGG"
     gene            2226..3098
                     /locus_tag="VFC15_17310"
     CDS             2226..3098
                     /locus_tag="VFC15_17310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM11970.1"
                     /translation="MNFKYADIEGMKVFYREAGDPKNPAMVLLHGFPSSSHMYRNLMA
                     ELADKFYLIAPDYPGFGNTDAPSPTQFAYTFDKLSETVEKLLVHIGVTKFTMFIQDYG
                     SPVGFRIAVRHPDWIEGIISQNGNAYEEGFTGVWDALRSALWKNRTPETEAPLAGFFT
                     ADGVKWIYSEGTRNPEKISPDNWSTDLFFLGRPENHRIQVDLFYDYRTNVEQYPRWHE
                     YLRKNQPPTLLVWGANDPIFTQEGGRAFQRDVKDAELHMLDTGHFALEDHCVEIATFV
                     RSFYDRKVRVKKAA"
     gene            3170..3814
                     /locus_tag="VFC15_17315"
     CDS             3170..3814
                     /locus_tag="VFC15_17315"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZM11971.1"
                     /translation="MFVQELWRYPVKSMGGESLATADVAERGIAGDRIIQVRNDSGRI
                     FTSRTRPGLLRHRAVLAENGEVLVDGCPWGTEEVARNVEEAAGKGTRLVRSDADDRFD
                     VLPLLVTTDGMFAAVGYDRRRFRPNLVIGGVDGLSERQWEGSQLRIGKVVIGMAALRS
                     RCIMTTFDPDTGNQDLNVLRRVQKEFQGVLGLNSYVVTPGRIAVGDPVELILRK"
BASE COUNT          859 a         1128 c         1107 g          795 t
ORIGIN      
        1 atatcaccac cctcgtttcc atcggaagcc ttcgaataga cactccccgt gagcccgctt
       61 gtacatatgt tgagatcaac gtcgagatca acgttgcgca tcccgcccca aatctccgac
      121 cgtccaatct ggtgtatctc cggcggcaag ggctccgcag agggcacggg cttttctatc
      181 cccgtcgaac agcggtcatc tggctggttg gtctggaaag tatctggcaa tggcggcgtc
      241 ccacgatgac agaaacacga ggtcaaagta ctgggagtct gtctggtcaa aagagctgac
      301 tctgcatgat atcccgaagc tggagtccgt gcggtgtgaa agggtcggaa tgaaatggat
      361 gccagaaact gggtaatact tcaaaagatc ggagagcggg acgcagataa cacacgccaa
      421 gcagagaccc agtcgatgag tcccctgcct gcaggacgat tctgactacc ctccgtggct
      481 ccgcaagatt cccagggttc gtagtctgga cgccagcgta ttcgggttta cgcccagcag
      541 ttccgcagcc ccaccagcac ccgacacctt gccgcctgcc tgcttcaacg ccttcaacag
      601 gttctcccgc tccagacttc tccattgcgc ctcggtgagg aaatcggcgg aatggttgga
      661 aactacagtc gctaaattgg ccgaaggcct atcctgcagc tgaaattcca acggagcacc
      721 acgcgaaaga atcacggcac gctctatcac atgctgcaat tcgcgcacgt tgcccggcca
      781 atcgtactgt tgcagtcgat ccagctcgcg ccggggcagc cgaggttcgg gagcgtgcat
      841 gcggacgcag gccaacttca caaggtgtgc cgccaactgc gggatgtctt ccctgcgctc
      901 gcgcagaggc ggaaggtcga gcgggaagac gctcaggcgg tagaacaaat ccaaacgaaa
      961 gcggccggct tcggattccg tgcgcaagtc ccggttggtg gcggcgatca cccggacgtt
     1021 cacccgacga gtgtgatcat cacccactcg ttcaaactcg ccttcctgca gtacgcgcaa
     1081 cagcttcgac tgaaggtcga gcggaatctc gccgacctca tccagaaaaa gagttccgcc
     1141 atcagccagt tgaaagcgtc cgatccgatc gcggatagcg ctggtaaacg atcccttgac
     1201 atgaccaaag aactcgctct caaacaactc cctggggatg gatccgcagc tgacgcggat
     1261 gaggggctgg tggccgcgcc ggcttcgctg gtggatggcg cgggcgataa gctccttacc
     1321 cgtgccggat tcaccctgaa tcagaacggc ggaatcggtc tgggccacca ggtcgatctg
     1381 ttgcagcacg cgcgtcaacg ccggactgtg tccaacgata tcgcggaagt cgcccgtttc
     1441 taccacctct tcgcgtaggt agtcgcgttc cagttccagt tgccggcgaa gcttgtcaat
     1501 ctgctggaag gcgcggcaat taccgatggc aactgcggcg tgagcggaga aactattcag
     1561 ccagtccaac gtgttcttgt tcggatgcac tctgaggaac acagccatca ccccgacgat
     1621 cgctcctcga aatcgcagtg gatggcctat caaaccttca atgcactcgc gctttgccca
     1681 accgcggtcg atagcccctc ccgtatccga tgcaatatcg gcaatcagaa cgggttcctc
     1741 agtgcgaacc atctgagcca gtttagtcgg cgaatccaac gaaactcggt gatgagaacg
     1801 attgacttgg ctccaatcgg caccaggctc caagggtcgg ccatcgctgg cgcgcatgtg
     1861 caaagtcatc tcgtcgcctg gctggccgca aaccgggcag tcgccatccc gatcaagaaa
     1921 ccagatccgt gccagcgcaa catgctcgtt ggccatgatc gccttgatga tctggccaag
     1981 taccgaatca ggtccctctg cctgggcgat cgagaggatg acgggaagga gatccatgac
     2041 gtcttgtgaa aaacaacaaa tttgtttcga tagccactta cttttgttga agttcactat
     2101 accgaagcac ctatatacat gcaagctatt gaaaactata gataagacgc ttggcagtcc
     2161 tcgtgcactt gttgtctgcg tacgcggcaa tcacgaattc tcgtcatcga aaggaacttg
     2221 acactatgaa tttcaagtat gcagacattg aaggcatgaa ggttttctac cgtgaagcgg
     2281 gcgaccccaa aaacccggcg atggttctcc tccatggatt tccatcgtcc tcccacatgt
     2341 accgcaacct gatggcggaa ttggcggaca agttctacct gatcgctcct gactacccgg
     2401 gcttcggcaa caccgatgct ccctcgccaa cacagttcgc ttataccttc gacaagctct
     2461 ccgagacggt ggagaaactg ttggtacaca tcggcgtgac gaagttcacg atgttcattc
     2521 aggactacgg ctcccctgtc ggcttccgca tcgcggttcg gcaccccgat tggatcgaag
     2581 gcatcatctc ccaaaacggc aacgcctacg aagaaggatt caccggcgtg tgggatgctt
     2641 tgcgcagcgc gctctggaaa aaccgcacac ccgagaccga ggcgcctttg gctggcttct
     2701 tcactgcgga tggtgtgaag tggatttaca gtgagggcac gcgcaacccg gagaagatca
     2761 gccccgacaa ctggagcacg gacctgttct tcctgggccg accggaaaac caccgcattc
     2821 aggtggacct tttctacgac taccggacga atgtcgagca gtacccgcgc tggcacgagt
     2881 acttgcgcaa gaatcaacct cccacgttgt tggtgtgggg ggcgaatgat cccatcttta
     2941 cacaggaagg tggtcgagcc tttcagcgcg atgtaaagga tgccgaactg cacatgctcg
     3001 ataccggcca cttcgcactg gaagatcact gcgttgagat cgcgaccttt gtgcggtctt
     3061 tctatgatcg caaggtgcga gtaaagaagg cggcctaggc aggttcaaac gaacgcggct
     3121 cgcagcgtga ccacactggc agcgagccgc ttcgggttgg agaagaaaca tgtttgtcca
     3181 ggaattgtgg cggtatccgg taaagtccat gggcggggaa tctctggcga ccgccgatgt
     3241 cgccgagcgc ggtatcgccg gtgaccgcat catccaggtg cggaatgaca gcggacgtat
     3301 cttcacttcg cgaaccagac ccgggctgct ccggcatcgg gcagtgctgg ccgagaacgg
     3361 cgaggtgctc gtcgatggat gtccttgggg caccgaggag gtcgctcgca acgttgagga
     3421 agcggcgggt aaggggactc gcctcgtccg aagtgacgcg gacgatcgtt tcgatgtcct
     3481 tcctctgctg gtcacgaccg atgggatgtt tgctgccgtg ggttacgacc ggcgacgctt
     3541 tcgcccaaac ctggttatcg gtggcgtcga cggcctctcc gaacgccaat gggagggatc
     3601 acagttacga attggcaagg tcgtcattgg catggctgct ctgcggagcc gctgcatcat
     3661 gaccactttc gatcctgata ccggcaatca ggacctaaac gtgctgcggc gcgtgcagaa
     3721 agaatttcag ggagtcctgg gactgaacag ttacgtcgtg acgcctggcc gaatcgctgt
     3781 cggcgatccg gtggaactaa tacttcgaaa atgatcgact tcaccactgc cgcgtttctt
     3841 ggcgttgact tgggctggta cgggaaaccg agcggcctgg cgtccattg
//
LOCUS       DASRKK010000716         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_759203, whole genome shotgun sequence.
ACCESSION   DASRKK010000716 DASRKK010000000
VERSION     DASRKK010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759203"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFC15_17320"
     CDS             complement(<1..1189)
                     /locus_tag="VFC15_17320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM11972.1"
                     /translation="MKLPSKCRRSGLLALAFAVLAATLATLSAQPAKPARSSPQWVRD
                     GVVYEIFPRNFSSEGNFNGITARLDELKDLGMTILWLMPIHPQGEKMRKGTLGSPYAV
                     RDYYAINPDYGTEADLKRLVSEAHQRGLKVIIDMVANHTAWDSVLMQHPEFYKQDASG
                     KIIPPVKEWTDVAGLNYANPKLREYMLAMLKHWIDPAGANLDGFRCDVAAEVPTSFWE
                     EARVELTKVKPDIIMLAEASKPELLVNAFDLDYAWPFHSSLNDILLRGAPASEFKRSW
                     EESQKQFHKGSLHLRFSDNHDEARAVARFSAKGALAASVLMFSLDGVPLLYNGMEVGD
                     ATESGDPALFEKLPIFWHPKERPPLRGIYQGLIKLRAEYPAFQNDRVVWLHNSDESNL
                     VTIM"
     gene            complement(1678..2148)
                     /locus_tag="VFC15_17325"
     CDS             complement(1678..2148)
                     /locus_tag="VFC15_17325"
                     /inference="COORDINATES: protein motif:HMM:NF025472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HZM11973.1"
                     /translation="MAGNLLALPACLVHLLEDQPQQTMPKIITAGAMMQCSFGVAPSA
                     LVLTPDIPTYATIPAATIMDNIPLKNIPPFGMCTTQSNPTVAAATAAALGVPTPAPCM
                     PVIPAPWAPGCPKIVINNIPALTETCKCQCAWGGVIGFTNPGLTKVGRTVIEIG"
     gene            complement(2355..4457)
                     /locus_tag="VFC15_17330"
     CDS             complement(2355..4457)
                     /locus_tag="VFC15_17330"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM11974.1"
                     /translation="MVSLVEQGWANQIEIARAFACSARTVRRHQRRFEAGGLAALGHT
                     GGYPPGRVRVAAARERWVQRLKNDGVGQREIARRMGVSEKAIRKLLRRLGWKATPLVQ
                     SQLALERAPRVDPNLSIPAKSEALPPVPGADPNLSAFTATAKESPVVSHDTDPSDRCG
                     DRLLARLGLLEDASPLFGSAAAVPRAGVLLALPVLIASGVFECAQRIYGSLGPAFYGL
                     RTSLLTLLLMALWRIKRPEGLKEYSPTDLGRVLGLDRAPEVKTLRRKLARLAAAGRAA
                     QFGQALAQQRVAAHGDAVGFLYIDGHVRVYHGKHALPKAHVARMRISMPATSDYWVND
                     MSGDPLFVVTAQANAGLVKMLPGLLKQIRSLGGQRRLTVVFDRGGFSPKLFQQILAAG
                     FDLLTYRKGRHPKIPQKRFRKYRTTLGGAASSYTLADQEVRLLKGRLRLRQVTRRMDN
                     GHQTPILTSRRDLPGAQVAYRMFDRWRQENFFKYLREEYALDALAEYATTPDDASREV
                     PNPAWAAADTQLRRAYAHLDRLQAEYGLEAWTNSEQQRRTMRGFKTAQGKLGRKLWDA
                     WEQVQRLEKRRAAIPKRVPVQSVTDKPVVKLAPERKHLTNVIKMLAYQSESDLLRLAA
                     PHYRRADDEGRTLIQSALASRADIEVTHEELRITLAAQSSVHRTRVVAELCKELNTAQ
                     TLFPGSRLRMRYAIREAS"
     gene            4742..>4975
                     /locus_tag="VFC15_17335"
     CDS             4742..>4975
                     /locus_tag="VFC15_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11975.1"
                     /translation="MSGVLESQTAVVSNCVVTGNSAGLGGGAFGGTLTDCTLVGNSAQ
                     DGGGAYEATLNNCTLSGNSATGYSHGGGVCGGVL"
BASE COUNT          926 a         1459 c         1552 g         1038 t
ORIGIN      
        1 gcatgatagt gaccaggttc gactcatccg agttgtgcag ccagacgacc cggtcgtttt
       61 ggaaggccgg gtattcggcg cgcagcttga tcagcccctg gtaaatcccg cgcaggggcg
      121 ggcgctcttt cgggtgccag aatatgggca acttctcaaa taacgctggg tccccagatt
      181 cggtcgcgtc tccgacctcc atgccgttgt aaagaagggg gaccccgtcg agtgagaaca
      241 tgaggaccga ggcggcgagc gcgcctttgg cgctgaagcg ggctacggcg cgcgcctcgt
      301 cgtggttgtc ggagaagcgc aggtggaggg agcccttgtg gaactgcttc tggctttctt
      361 cccaggaccg tttgaattcg gaggcgggag cgccgcggag caggatgtcg tttagagagg
      421 agtggaaggg ccaggcgtaa tcgagatcga aggcattcac gagcagctcg ggcttgctgg
      481 cctcggcgag catgatgatg tccggcttga ccttggtgag ctcaacgcgg gcttcctccc
      541 agaagctggt gggcacctca gcggcgacat cacagcggaa gccgtccagg ttagccccgg
      601 cgggatcgat ccagtgcttg agcatcgcaa gcatgtattc gcggagcttg gggttcgcgt
      661 agttcaatcc cgccacgtcc gtccactcct tgaccggcgg gatgatcttg ccgctggcgt
      721 cctgcttgta gaactccggg tgctgcatga gcacgctgtc ccaggcggta tggttggcca
      781 ccatgtcgat gatgaccttc agcccgcgct ggtgcgcctc ggacaccagg cgcttcaagt
      841 ccgcctcggt gccgtagtcg gggttgatcg cgtagtagtc gcggacggcg taagggctgc
      901 cgagcgtgcc tttgcgcatc ttctcgccct gcgggtggat gggcatcagc cagagaatgg
      961 tcatcccaag gtcctttagc tcatccaggc gcgcggtgat gccattgaag ttgccctcgc
     1021 tcgagaagtt gcgggggaag atctcgtaca ccactccatc gcgcacccat tgcgggctgg
     1081 accgcgcggg tttggcgggt tgggcggaga gggtggcgag cgtggcagcc agcacggcga
     1141 aagccagagc cagcaatcct gatcggcggc acttggaggg cagtttcatg gtttgttcct
     1201 ttcccggttt cactgagtca ttgcggttat gggagcaccg tgacccgcgc atcaagcgca
     1261 cgttcgccac aaaatggggg cagggcttta ggagtgtgag cttgtttcgg ttcgcggcac
     1321 attgcggaca tggattctaa atagccagcg cacggccgga tttcaaggat tatgctcctg
     1381 tattacttgg gcaatttgct cccatgcgcc cgctctgggg ctttgggcgg atcgcgtgct
     1441 gcgctgagtt tctcttcggc aactcgctgt tcgccgacga cttgctgcaa tagctaggcc
     1501 ccgggcatga cggcgtctgg cgcacaccgg ttgcgcgcct tcatttgagg ttgtggcccc
     1561 agcgattagc ggagcgcgaa ccaacattgc ttcgatccag aaagtgcgca cagtggagaa
     1621 acttcgttct gacaccctac tcttcctgct ctgtccacgt tcattccacg gagctgatca
     1681 gccaatctcg atcaccgtcc gtccgacctt agtcaggccg ggattggtga acccaatcac
     1741 gccaccccag gcgcactggc atttgcaggt ctcggtcagg gcgggaatat tgttgatcac
     1801 gatcttggga catcccggcg cccacggtgc gggaatcacc ggcatgcagg gtgccggtgt
     1861 tggcacccct agagcggcgg cggtggcggc agcgaccgtg gggttggact gcgtcgtaca
     1921 catcccaaac ggaggtatgt ttttgagggg gatgttatcc atgatggtcg cggcggggat
     1981 ggtggcgtac gttgggatat ccggcgtgag gaccagcgcc gacggcgcaa cgccgaagct
     2041 gcattgcatc atggccccgg cggtgatgat ttttggcatg gtctgttgtg gttggtcttc
     2101 cagaagatgc accaggcacg ctggaagcgc gagtaaattc ccagccaaat ccgacttctt
     2161 gataatggtt ggtccactcc agttattcct cgctgttgca gccgctattt cagatattca
     2221 atgtcgagtc tgcctccccc catgcagaca agcggccacc atcgactcgc caacgatttt
     2281 ataacataac gataaccgac cagtttgagt ccagaactcc tgacataggg gagttaaaaa
     2341 tgtccgcttt ttcgtcacga cgcctcgcgg atggcgtagc gcattcgcag tcgcgatcct
     2401 gggaaaaggg tttgggccgt gttcagttcc ttgcagagtt cggcgaccac tcgcgtgcga
     2461 tgaaccgagc tttgtgcggc cagcgtgatg cgcagttcct catgggtcac ttcgatgtcc
     2521 gccctgctgg ctaaggcgga ttgaatcagt gtccggcctt cgtcgtcggc tcgacggtaa
     2581 tgaggggccg ccaaccggag caagtcgctc tcagactggt aagccagcat cttgatcaca
     2641 ttggtcagat gcttgcgttc gggcgccagt ttgacgacgg gtttgtcggt tacactttga
     2701 actggcactc gctttggaat ggcggctcga cgtttctcca gccgctggac ctgttcccag
     2761 gcgtcccaga gtttgcggcc cagttttccc tgagcggttt taaatccgcg catcgtgcgg
     2821 cgctgctgct ccgagttggt ccacgcctcc agtccgtatt ccgcttggag tcgatccaga
     2881 tgcgcatagg cccgacgcag ttgggtgtcc gcggcggccc aagccggatt gggcacttcg
     2941 cggctggcgt cgtccggcgt tgtggcatat tcggccagcg catccaacgc atactcctcg
     3001 cgcaggtatt tgaaaaagtt ctcctgtcgc cagcgatcaa acattcgata cgctacttga
     3061 gcgccgggca aatcgcggcg tgaagtcagg atgggcgttt ggtggccgtt gtccatccga
     3121 cgggtcacct gcctcaatcg caacctgccc ttgagcagac gcacttcctg gtcggctaaa
     3181 gtgtagctgc tggcggctcc gcccagagtg gtgcggtatt tccgaaagcg tttctgcggg
     3241 attttgggat ggcgtccttt gcgataggtg agcagatcaa aaccggcggc gaggatctgt
     3301 tggaagagtt tcgggctaaa cccgccgcga tcaaagacaa cggtgagccg ccgctggccc
     3361 cccagactgc ggatttgttt cagcaatccg ggcagcatct tgaccaaacc ggcgttggct
     3421 tgggccgtga cgacgaataa cggatcgccg ctcatatcat tgacccaata gtccgatgtc
     3481 gccggcatgg agatgcgcat gcgcgccacg tgggctttgg gcagcgcgtg tttgccgtgg
     3541 taaacccgca cgtgcccgtc aatgtagagg aaccccaccg cgtcgccatg cgcggcgacc
     3601 cgttgctggg ccagagcttg gccgaactgg gcggcgcgtc cggccgcagc caggcgcgcc
     3661 aacttgcgcc gcagcgtttt gacttccggc gcccgatcca atcccaacac gcgacccaaa
     3721 tcggtgggtg agtattcttt caggccctcg ggacgtttga tgcgccacag agccatcagc
     3781 aagagcgtga gcagactggt gcgcagtcca taaaaggctg ggcccaggct gccgtagatt
     3841 ctctgtgcgc actcgaaaac gccactggcg atcaggaccg gcagcgccaa gagcactccg
     3901 gcccgtggaa cggccgcagc agaaccgaac agaggtgagg catcctcaag caatcccagg
     3961 cgcgccagca accgatcgcc gcaacgatca ctcggatcgg tgtcgtggct gaccaccggg
     4021 ctttctttgg cggtggcagt aaaagcggac aggtttgggt ccgcacccgg aactggcggc
     4081 aaagcctcgg atttggccgg aatggacagg ttggggtcca ctctcggtgc cctctccaag
     4141 gccagttggc tctgaacaag cggtgtggct ttccatccca aacgtcggag gagtttgcgg
     4201 atggctttct cactgacgcc catccgccgg gcaatctcgc gctgacccac gccgtcattc
     4261 ttcagtcgtt gaacccaccg ttcccgtgcc gcggccaccc gcacacgtcc aggcggataa
     4321 ccacccgtat gtcccaacgc ggctaagccg ccggcctcaa agcggcgctg atgtcgccgc
     4381 accgtgcgcg ccgaacaggc aaacgctcgc gcgatctcga tttggtttgc ccagccttgt
     4441 tccaccaaac tgaccatggc atgagcctca gccatatagt cgcttgacgc atactgtgcc
     4501 aggaccattc ccgaaacgac aaccacacgg tggccgtctt gacttctcag cagacagcgc
     4561 tcgttgatga cacgcagaca ttccgggttg tgagcttcag gaaatagatc gtctttttgc
     4621 ggggccgcca taccgggcga gcatacccgg cgtccaaaaa agcggacagg tttgggtccg
     4681 caccccgcac caccacgaga cgagctacaa gcccgccaat atgcaggaaa ttgagatccc
     4741 tatgtcagga gttctggaat ctcaaactgc agtagtttcc aactgtgttg tgaccggtaa
     4801 ctctgccggc ctcgggggcg gggcgtttgg cggcaccctg actgactgta cactcgtcgg
     4861 caattccgcg caagatggcg gcggggccta tgaagcgaca ctgaacaact gcacgttgag
     4921 cggaaactcg gctacaggat acagccatgg cggcggggtc tgcggaggcg tactg
//
LOCUS       DASRKK010000717         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_855926, whole genome shotgun sequence.
ACCESSION   DASRKK010000717 DASRKK010000000
VERSION     DASRKK010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855926"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFC15_17340"
     CDS             <1..396
                     /locus_tag="VFC15_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11976.1"
                     /translation="AGLLARWIPEAEIRSENALTNLAYRKEYDAVVTLRLGSREVTFA
                     LEYERTPKSRSKYDKIAEDMCREENVDHFLYLTANEHLLRFASWSFRDLQRRVYFGLV
                     TDWHRQLLDMPAFSWKVKRYVPLKTLLAQ"
     gene            complement(1666..2475)
                     /locus_tag="VFC15_17345"
     CDS             complement(1666..2475)
                     /locus_tag="VFC15_17345"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZM11977.1"
                     /translation="MPESQTTTTMVANYQNILFDTEGQIAIVTLNRPQRRNALSLELM
                     TELVDCLNEIGRDRSLRVVLLRAAGKVFSSGHDLSEMVGRNINEYRQVFDVCTELMTR
                     VQSIPQPVIAEVQGIATAAGCQLVATCDLAVASDQAAFATPGVKIGLFCTTPMVALSR
                     AVGRKRALQMLLTGEMIPAVTAAEWGLINMVVPAEELAEQCHNLATRIAEASSLTVAL
                     GKQAFYTQIDIDQPKAYAYAKEVMSMNALAADAQEGISAFLEKRRACWTGR"
     gene            complement(2475..>3011)
                     /locus_tag="VFC15_17350"
     CDS             complement(2475..>3011)
                     /locus_tag="VFC15_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl-CoA synthetase"
                     /protein_id="HZM11978.1"
                     /translation="GTDMRVVDEDMRDVPADGLKVGEVVMRGNNVMLGYYREPDATAT
                     AFRGGWFHSGDLAVMHADGYIEVRDRAKDIIISGGENISSLEVERVLYEHPAVLEAAI
                     VAWPDEKWGEVPHAYVLVREDVVVSEQDLIEFCRERLAHFKCPKAIHFGALPKTSTGK
                     IRKNVLRHQSRTAVEGDS"
BASE COUNT          595 a          868 c          865 g          683 t
ORIGIN      
        1 gccgggctgt tggcccgatg gatcccggaa gcagagatcc gctcggagaa cgcactgaca
       61 aacctggcct acaggaaaga atacgacgcg gttgtcacgt tgcggctcgg cagccgcgag
      121 gtgacatttg cgttggagta tgaacggacg cccaagtctc gaagtaaata tgacaagatc
      181 gcggaggaca tgtgccggga agaaaacgtg gatcacttcc tgtacttgac tgccaacgag
      241 catttgctga ggttcgcgtc ctggtccttt cgagatcttc agcggcgtgt ctatttcggg
      301 cttgtcacag actggcaccg gcagttgctc gacatgccag ccttcagctg gaaggtgaaa
      361 cggtatgttc cactcaagac tttgcttgct caatgaaggc gcggctccac gcgaactttc
      421 cccagtttgg ccccgaacga caatggcgac gcgacggaac caactcaata gtctcttgga
      481 acaaatgaga cttggcctga ggctgcagaa agtggcggta ctttctgacg attctggagc
      541 caaggtttaa gcggtatgcc taccctttgc ctgcctattg tctgctcatt gcctgctcgg
      601 aggtagtcgt aactattcgt attctcaatg agatgcggct cgactactcc cctcttcatg
      661 caggttttgc ctataaaacg accgaacagt tgtggcattg gcgcgtgcta ttgcgatgca
      721 ttgtgtgcag ccaggatgac gcagttcttg tacatcctga gttggtcgtt ccggttaggt
      781 gttgcggcag ttgggcgaaa ctgtgcgcgc gcaagttgca caagctgggt gcggcgagac
      841 gcgcgggctg tgcgcacagc agtggttcgg tttgggcgat gaatgtaccc ggctatcgtt
      901 cgagcggcgc ggcgctgtgt tgtggactga tccaccagta tcggggtgca cacgtgtcgc
      961 aaattgttgt tccgtggtcg ctggttgtgg ccgaaccgtg cgcatgggct ggtgcaagac
     1021 gagtggcaag gtgcacagct cgtttgagat tcgaactgcg tggtcggtct gcaccgtcgt
     1081 gtgcacactg ctgtgtacgg atgcgcatta gatggacgcg gctgccgatt ccggttgcaa
     1141 cgagactgcg catctgatgt gcgaacgttc aaactacggc ttgtgctggt tgcaacggaa
     1201 gtcatggcat tgtggcgatg ctcgggtgat ggacgtgctc ttaggactca tgcacagctg
     1261 gcgatggagc ggcgaaccac acattccggt ttgtccgtcg gtcgggagga atctcggtga
     1321 attgtcagcg gcagatctcc cacaagaaga cttccggaca actaccgggg ctagcctcca
     1381 gtgtgggtcg ctgttttgag cgccgacaac tgccggtttc tccttgcttc ggtcgtaatc
     1441 cccagcgtgg ccacgacgga tcacagccga ctttgtccct gcgggccagt atccagccaa
     1501 ctctcccgcg gcatgcacac gaagttcttg atcggtgcca gtcaatttga gatccggtgc
     1561 aaaacgaact tcctcggagc aaaactcggt gcgatcgtcg aggaccgttc gcaatgttcg
     1621 tgatctagac ttttaggttt caaaagtaca acacggagga atactctacc ttcccgtcca
     1681 acacgcacgc cgtttttcca gaaatgccga gattccctcc tgcgcgtcgg cggcgagcgc
     1741 attcatgctc atcacttctt tggcgtaggc ataggctttt ggctggtcta tatcaatctg
     1801 cgtgtagaac gcctgcttgc ccagagcgac cgtcagcgaa ctggcttcgg caatgcgagt
     1861 ggcgagattg tggcactgct ccgccagttc ctcggcagga acgaccatat tgatcagtcc
     1921 ccattcagcc gccgtcactg cggggatcat ctcgccggtc aacaacatct gcagtgcccg
     1981 tttgcgcccg acagctcggc tgagggccac catgggtgtg gtgcagaaga gtccaatctt
     2041 cacccctggc gtagcaaacg ccgcctgatc ggaggccacg gccaaatcgc aggtcgccac
     2101 cagttggcat ccggctgcgg tcgcaatgcc ttgcacctct gcgatcactg gctgaggaat
     2161 cgattgcaca cgtgtcatca gctcggtaca gacatcgaac acctggcggt actcgttgat
     2221 gttgcgtccc accatctcgc tcaaatcgtg accggacgag aagactttcc ctgccgcccg
     2281 caacagcacg acacgcaaag agcggtcgcg cccgatttcg ttcaagcagt cgaccagttc
     2341 ggtcatcaac tccagcgaaa gcgcattgcg ccgctgcggc cggttcagcg tcacgatcgc
     2401 aatctgtccc tcggtgtcga acaggatgtt ctggtaattg gcaaccattg tggtggtggt
     2461 ttgtgattca ggcatcacga atctccttct actgcagtgc gcgattggtg gcgcagcacg
     2521 ttcttgcgga tcttgcccgt cgatgttttg ggcaatgcac caaagtgtat cgccttcggg
     2581 catttgaagt gcgccagacg ctcgcggcag aactcgatca ggtcttgctc gctgaccacg
     2641 acatcttccc tgaccagcac ataggcatgc ggcacttcgc cccacttctc gtcgggccac
     2701 gcgacaatcg ccgcctccaa cacggctgga tgctcgtaca acacccgctc cacctcaagc
     2761 gaagaaatat tctcccctcc ggagatgatg atgtccttag cgcggtcacg cacctcgata
     2821 tagccgtcgg cgtgcatcac cgccagatct ccggagtgga accagccgcc acggaatgcg
     2881 gtcgcggttg cgtctggctc tcggtagtag cccagcatca cgttgttgcc gcgcatcact
     2941 acttcgccca ccttgagtcc gtcagcgggg acgtcgcgca tgtcttcatc caccacccgc
     3001 atgtcagtcc c
//
LOCUS       DASRKK010000718         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_171589, whole genome shotgun sequence.
ACCESSION   DASRKK010000718 DASRKK010000000
VERSION     DASRKK010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171589"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VFC15_17355"
     CDS             complement(<1..1315)
                     /locus_tag="VFC15_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZM11979.1"
                     /translation="MKLGRNHPCSCGSGKKYKHCCLNAVSVQAAAPADLTWRRLRSLL
                     DGYATEMLRFIAEAYGPLAVHEAWNEFMCDGGLEFDPDTPLMQLFMPWFFHCWSPDPI
                     ATMVGEKSLHNVIPTAAYLSSKGRRLDPLLRRYLESLLSAPFTFFEVLACDPGTGMTL
                     RDVMTQEEHAVTEHGASQGVQPGDLLFGQLASVDRLTMLEAGNGFAIPPMEKAPIIEL
                     RAQIASAHPVITHQVLRDWDFELLDLFHEIADRLYNPRLPTLQNTDGDPLSLHKIVFD
                     LNVVPQDAFDALKHLALDELDEDLLADATRDFEEKLTRVRFSWKKRGNKKHAGWDNTV
                     LGSIEIDGTRLLAEVNSEARANAIRKKIETALGEGVRYRVREIQSPERMLADLRAAYG
                     DGAASRESDRLAEIPEVRAKIAEMMAAHWEHWVDQPLPILGNRTPM"
     gene            complement(1371..1925)
                     /locus_tag="VFC15_17360"
     CDS             complement(1371..1925)
                     /locus_tag="VFC15_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11980.1"
                     /translation="MVTAKPKKTSPTWIDVKAKVADFDRAGLLGILQDLYAGSKDNQA
                     FLHARFGLGGDVLEPYKVIINRWLWPDVLKNQFTSVSAAKKTIADYNRATGQPEGLAE
                     LMVFYCECAAGFSGSVGYEDGVYFDALMSMFEQALKLSVTLPVDQRDVLLDRLDDVRR
                     ISNDFGYGLGDGMNMLFAKHGVDG"
BASE COUNT          447 a          609 c          545 g          432 t
ORIGIN      
        1 ccatcggcgt gcggtttcct agaatgggga gcggctgatc cacccagtgc tcccagtgcg
       61 ccgccatcat ttcggcaatt ttagcgcgca cctcaggaat ttcggcgagg cgatcgctct
      121 ccctggaggc cgcgccatca ccgtaggccg cccgcaggtc ggccaacatc ctctccggcg
      181 attgtatctc tctcacacga taacgaacgc cctccccgag cgccgtctcg atcttcttgc
      241 gtatcgcgtt ggcgcgcgcc tccgaattga cctctgctag gagacgcgta ccgtcgatct
      301 cgatcgaccc gagcacggta ttgtcccatc cagcgtgttt cttgttgccg cgcttcttcc
      361 aggagaaacg tacgcgcgtc agcttctcct cgaaatcgcg tgtagcatcc gccagcaggt
      421 cttcgtccag ctcgtccagc gcgaggtgct tcaacgcatc gaaagcgtcc tgtggtacga
      481 cattgaggtc gaatacgatc ttgtgcagcg aaagcgggtc gccgtccgtg ttctgcagcg
      541 tcggcagccg cggattatag aggcgatcag cgatttcgtg aaataggtcg agcaactcga
      601 aatcccagtc ccgcaggact tgatgggtga tcaccgggtg cgctgacgcg atctgagcgc
      661 gcagctcgat gatcggcgct ttctccatcg ggggaatggc aaagccatta cccgcttcga
      721 gcatcgtcaa tcggtctacc gacgccagtt gcccgaacaa caagtcgcca ggctgcaccc
      781 cctgcgacgc gccgtgctcg gtaactgcgt gctcctcctg cgtcattaca tcgcgcaagg
      841 tcatcccagt tccgggatcg catgccaaca cttcgaagaa cgtaaacgga gcggacaaaa
      901 gcgattcgag atagcgccgg agaagcgggt cgagccgacg ccctttgctc gacagatatg
      961 ctgcggtcgg gatcacgttg tgcagagact tttccccaac catggtagca atgggatctg
     1021 gtgaccaaca atggaagaac catggcatga acaactgcat gagcggcgta tcgggatcaa
     1081 attcgagacc gccatcgcac atgaactcgt tccaagcctc atgtaccgcc aatgggccgt
     1141 aggcttcagc gataaagcgc agcatttcgg tcgcgtaacc atcgagcagc gaacgcagcc
     1201 tgcgccaggt aagatcagcg ggcgcggctg cctgaacaga tactgcattg agacagcagt
     1261 gcttatattt cttgccgcta ccgcaagagc agggatggtt gcgaccaagt ttcatttgtc
     1321 agttttccgt atggctttca tggattacga agacctcgat tcgatcgtgc tcaaccatca
     1381 actccatgct tcgcgaagag catgttcatg ccatcaccaa gcccgtagcc aaagtcattg
     1441 ctgatgcggc ggacatcatc gaggcggtca agcaagacat cacgctgatc aaccggcagg
     1501 gtaaccgata actttagcgc ctgctcgaac atgctcatca gagcatcgaa gtagactcca
     1561 tcttcatagc cgacagagcc gctgaatcct gccgcgcatt cgcaatagaa caccatcagc
     1621 tcggccagtc cttcaggctg cccggtagcc ctattgtaat ctgcaatcgt cttcttggcc
     1681 gcggaaactg acgtgaactg attcttgagc acgtcaggcc aaagccaacg gttgataatg
     1741 accttgtacg gttcaagcac atctccaccc agaccaaacc gggcgtgaag aaatgcctga
     1801 ttgtccttgc tgccggcata aaggtcctgc aaaatcccaa gcagaccggc gcggtcaaag
     1861 tcggccacct tagcttttac gtcgatccaa gtgggcgagg tctttttcgg cttggcggtc
     1921 accatcgtgc tccagattgt agcggcgcga cgctactcca gaatcagatt ttctagcaat
     1981 tggtatgaat tgtcacgcct ccccaattcc ggggttgcga gccaatcgaa ggg
//
LOCUS       DASRKK010000719         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1443848, whole genome shotgun sequence.
ACCESSION   DASRKK010000719 DASRKK010000000
VERSION     DASRKK010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443848"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFC15_17365"
     CDS             <1..193
                     /locus_tag="VFC15_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM11981.1"
                     /translation="GKTAEGRHFGGPSLVSEKTTAASPDDVRNIISNGKGRMPKYAGK
                     LTSEEIDTLVQQIKALNKK"
     gene            580..951
                     /locus_tag="VFC15_17370"
     CDS             580..951
                     /locus_tag="VFC15_17370"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HZM11982.1"
                     /translation="MIKHEILQPDGILIVEPLSALAAEDFQALTTEVNEYLAGHPDLR
                     GILIHARVFPGWDSFAAFVAHMRFIRDVHTRVERVALASDSPIAAVGPAIAKHFVRAQ
                     FRHFPYAEYDDALQWLKAPAV"
     gene            1129..2055
                     /locus_tag="VFC15_17375"
     CDS             1129..2055
                     /locus_tag="VFC15_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM11983.1"
                     /translation="MENAISCRGLRKTYDGKVEAVRGLDLAIQAGECFGLLGPNGAGK
                     TTTIEILEGLLTPTSGEVEILGMTWQAHEQELREWLGISLQETRLSEKLTVRETLQLF
                     ASFYHQPNSVPAILEELSLGEKADALVGKLSGGQKQRLAVATALVGNPKILFLDEPTT
                     GLDPQSRRQLWDIIRQFQQRRGTVLLTTHYMDEAERLCDRLAIIDHGQIIAAGTPSEL
                     IARLSGHHVVEFALSDAVNDDGVWSRLPGVQAVRNEDGHFSLQVEEPHETIPALLSVV
                     QQKGARLVHLTTRQASLEDVFVQLTGRHLREE"
     gene            2055..>2438
                     /locus_tag="VFC15_17380"
     CDS             2055..>2438
                     /locus_tag="VFC15_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11984.1"
                     /translation="MAYLETPPTPAVAASKTVTNGRWAGYRHLLGARMKELWREPEVI
                     FWVFVFPLLLALGLGIAFRNKPADITTIAVVANSHAQQVIDLLQRSPAKTAIHADVLD
                     ANVAFDRFRLGKYALVVTADDSGLHY"
BASE COUNT          572 a          689 c          701 g          476 t
ORIGIN      
        1 cggtaaaacc gcggagggcc gccacttcgg tgggccgtca ctcgtctccg aaaaaaccac
       61 tgccgcatcg cccgacgacg tgcgcaacat cattagcaac ggcaaaggcc gcatgcccaa
      121 gtacgcgggc aaactaacat cggaagaaat cgatacgctg gtccagcaaa tcaaagccct
      181 aaataagaaa tgaatttccg atagggttcg ttgttcctgg cgttggcatc ggtcggaaag
      241 gaaccccgcc ggataagaaa tcagtgccgc caccaaattg aacccggcat aaatcaggat
      301 ggtcagttca accgaagcgc caatgctctt tgtttgcagg atcagaaagg aaaatcagca
      361 ccgaaaaagg tcacctcgtc agagaagaac tctactggtc gccgtccaat gagtccagcc
      421 atgcgcaaga aattatcggc gatgatgaaa gcgcgttggg ctgcaaggaa gaagggatcg
      481 tagaacgcat agatttgttg cacgtgagat acgagcaaca gagaatagct atggagtgga
      541 actgggggcc cccgctggca aattcagagt gaagcagaca tgatcaagca cgagattctg
      601 cagcccgacg gcattctgat cgtcgagccc ctttcggcgc tggcggcgga agacttccag
      661 gcactgacca ccgaggtcaa tgaatacctg gcgggacatc ccgacctgcg cggaattctc
      721 attcacgcgc gcgtgtttcc cggatgggac agcttcgcgg cttttgtcgc ccatatgcgc
      781 tttatccgcg atgtccacac tagggtcgag cgcgttgccc ttgcctccga cagccccatc
      841 gcagccgtcg ggccagccat cgcaaaacac ttcgtccggg cgcaattccg gcactttccc
      901 tatgctgagt acgacgacgc cctgcaatgg ctaaaggccc cagcagtgtg aacgaaaaag
      961 gggaacctgg cggaccttga tcacgcgagc gctgggtccg gctctgctat agggtggacg
     1021 cggcgcccag agtattctca ggatgggtct aaatcgaaga aagtcccgct ctgtccaagg
     1081 atcgtgcctc tcgtagagcg accgcaatgt cccgtatcat tgcccgatat ggagaatgcc
     1141 atcagttgcc ggggcctgcg gaagacctac gacgggaagg tcgaagctgt tcggggcctc
     1201 gacttggcaa ttcaagccgg cgaatgtttc ggtctcctgg gcccgaatgg agccggcaag
     1261 accacgacca tcgaaatcct ggaagggctg ctgacgccca cctccggcga ggtcgagata
     1321 ctgggaatga cgtggcaggc acacgaacag gagttgcgcg aatggcttgg gatctctctc
     1381 caggaaaccc gcctctctga gaaattaacc gttcgcgaaa cgctgcaact gtttgccagc
     1441 ttctatcacc agccaaactc ggttccggcg atcctcgaag aactatcact cggcgagaag
     1501 gccgatgcat tagtgggcaa gctctccggt gggcagaagc agcgactggc cgtcgcgacc
     1561 gcgcttgtgg gcaatccgaa aatccttttc ctcgatgaac caaccactgg attggatccc
     1621 caaagccgtc gccagctttg ggacatcatt cgtcagtttc agcaacgcag agggaccgtg
     1681 ctactgacga cgcactacat ggatgaggca gagcggctat gcgaccggct ggccatcatc
     1741 gatcacggac agatcattgc cgcaggcacg ccgtcggaac tgattgcacg cttgagcggg
     1801 caccatgtag ttgaatttgc actcagcgac gcagtgaatg acgatggcgt gtggagtcgt
     1861 cttcccggcg tgcaagcggt gcggaatgaa gacggacatt tctcattgca agttgaagaa
     1921 cctcacgaga cgatacctgc tctgctttcc gtagtacagc agaagggggc gcgcttggtt
     1981 cacctcacaa cccgccaggc gagtcttgag gacgtgttcg tacaactcac cggccgtcat
     2041 ctgcgggagg aataatggct taccttgaaa cgccaccgac gccagcggtc gctgcatcaa
     2101 agacggtcac gaacggacgc tgggcgggat accggcactt gctcggagcc cgcatgaagg
     2161 aattgtggcg ggagccggag gtcattttct gggtgttcgt tttccccctg cttctggcgt
     2221 tgggactcgg tatcgcattt cgtaataaac ctgccgacat aaccacgatc gctgtcgtag
     2281 caaattccca cgcacagcag gtgatcgatc tgttgcagcg ttccccggca aagacggcca
     2341 ttcatgccga cgtgctggac gccaacgttg cattcgatcg cttccgcttg ggcaagtatg
     2401 cgctcgtcgt aacagcggat gatagcggac ttcattat
//
LOCUS       DASRKK010000720        15537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1247379, whole genome shotgun sequence.
ACCESSION   DASRKK010000720 DASRKK010000000
VERSION     DASRKK010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15537
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247379"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFC15_17385"
     CDS             complement(<1..487)
                     /locus_tag="VFC15_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HZM11985.1"
                     /translation="MLSPFAKQQDVRPFELPGVSIFTGNELDPTDEPDAALIFGGDGT
                     VHRHLGTLVLKRTPTLAVPMGSANDFAATLGIDTVEQALTAWKQFCETRENSRLIDLG
                     TIQPLHPVEASDMLPALRDDDPDPWEGDSLETLHFVPDGPRRDLPPLGPRIEQSQARR
                     WI"
     gene            641..1165
                     /locus_tag="VFC15_17390"
     CDS             641..1165
                     /locus_tag="VFC15_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM11986.1"
                     /translation="MRLGEKLRYLRLVEGTLRGMEREMTQHELAVALKKELGRGLSQS
                     YLSQIESGARPHLTNASRMLLARYFNVHPGYLVDDPEGYHTELTSDLRAREDRLDLWL
                     INGAEQFARDPELSQALIEVAKYKDSRKCLVLLGAILEAPELVERLTEVLKPNLSQPL
                     HRGSGKRRSAGAKL"
     gene            1162..1752
                     /locus_tag="VFC15_17395"
     CDS             1162..1752
                     /locus_tag="VFC15_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11987.1"
                     /translation="MTWAMFYLVCFLVGVTLSALSFLGGSFHLPHFHVHVPHVHVPHG
                     GAHVGTGGEMPFLNFGTVTAFLAWFGGSGYLLTRYSSFMVAGVLTLAVIAGLVGAMIV
                     FWFVAKLLLKHDRELDPADYERVGVLARISSPVREGGTGEIIFSQEGTRNTCGARSEN
                     SEALPRGTEVIVTRYEHGIAYVRRWEELAEKDAASS"
     gene            1770..3239
                     /locus_tag="VFC15_17400"
     CDS             1770..3239
                     /locus_tag="VFC15_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZM11988.1"
                     /translation="MGFHIPTEVLIVAWTAVAAVVMIVAVFARLYRKAGPNEVLVVYG
                     FRGTRVVKGHGTVIFPMVETYRELSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVK
                     SDPESVLTASEQFLTKKPPDREALIRLVMEGHLRGIIGQLTVEEIVKQPEMVADRMRS
                     TCADDMNKMGLEVISFTIKEVRDKNEYISNMGRPDVARIKRDADVATAEAERDTAIRR
                     AIALRESAIAKAQADQERVAAETASLARQAEAQRDLEVKKATYNELVKRQQAQADKAY
                     EIQTNIMQQQVTAEAVKVQQVEKEQQVKVQEAEILRREKELIATVLKGAEIERKRIET
                     LAEAERQRLTVEAEGHASAIRAQGEAEAEIIFKKGDAEARAMNLKAAAYQEYNQAAVY
                     DKLITGLPEVVKALASPLNNVDKITIVSTGNGTSAGMHKITGDITEMAAQVPALFEAL
                     SGMKMSDLLGKIRTIGEKSPPELPPAGGDGSSNSAGSSK"
     gene            3381..4040
                     /locus_tag="VFC15_17405"
     CDS             3381..4040
                     /locus_tag="VFC15_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZM11989.1"
                     /translation="MALLDRVATLVRANLNDLVDRAENPEKMLKQVILDMENQFIQVK
                     TQVAIALADLHLLKKKKQENADKHAEWMRKAELAVEKKDDELARAALERSMSFQQLAE
                     SFEEQISDQEAQVEALKSALKKLEVKLSEARAKVDLLIAQHRRSRAVNRAADAQQMPG
                     GENLTFDRMRSKVAREEALGKAKGELLGDDIDGRFHALEREQKIDALLEEIKAKKRLS
                     A"
     gene            4181..5047
                     /locus_tag="VFC15_17410"
     CDS             4181..5047
                     /locus_tag="VFC15_17410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM11990.1"
                     /translation="MKRREFIVNTSLGLGAAWLARNSILNALAETQLPRKYSASDTVT
                     LGKTGIKTSRLAMGTGTVGYAKHSNQTALGVAGLSGLLLNGYDQGLRFFDAADSYGSH
                     PHVAAALKQLPRDKVTVMTKSPSRTPQEMRADLDRYRKELGTDYLDIVLMHCMTEGDW
                     TERFQGTMDVLSEAKQKGIIRAHGCSCHTIEALRAAAKSPWAEVDLVRINPIGSHMDD
                     DPATVVSVMQQMKSSGKGLIGMKILGQGDLRTRQSEAIKYALSLNLLDAFTIGAESKS
                     EQDNLIQRIAAA"
     gene            5191..5907
                     /locus_tag="VFC15_17415"
     CDS             5191..5907
                     /locus_tag="VFC15_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11991.1"
                     /translation="MQQKTVKLIALAFLVQVVSLGATFQAQAQAEKTSYPAMAPLDQY
                     LMPDRNSEIALARTGAPASISDAAEVMVLGPQGYSTAAKGTNGFLCIVERSWGASTDD
                     PNFWNPKIRAPICFNAPAVRTFVPIFLMKTKLVLAGKSKPEIVAATTSAFDKKELPAL
                     DPGAMCYMLSKQQYLDDKATHWHPHLMFFVAGDAAKSWGADLPGSPIIAANDPEERAT
                     IFMVVVGKWSDGSPAPQQMH"
     gene            complement(5946..6692)
                     /locus_tag="VFC15_17420"
     CDS             complement(5946..6692)
                     /locus_tag="VFC15_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM11992.1"
                     /translation="MRSTWFAITLMLFFVLPALSQVGGQTQLEGQIVGTVLDEHNQPV
                     SGAHVAGLMLVEHKDPTQDALCNNVFFINTGKTGNCLAITDETGQYHVDHLPMGTFLL
                     IASKEEDGYPRYFEGRERQTVTLTPGYPVRTVIAKLGQQEGILAPSVKDKVTGEPVKK
                     FRVKFEMENADPSREAGRSIMFGRAMTRTCIPVDRGLSIEVSATGYKKWVYVDPSNPS
                     EKFPVRVQRGEVKSLDIELEPDEENTQTAR"
     gene            complement(6789..7928)
                     /locus_tag="VFC15_17425"
     CDS             complement(6789..7928)
                     /locus_tag="VFC15_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11993.1"
                     /translation="MHRLLSSVILFVLLLGLPAQVPAQSAERWSEQRANTWYQQQPWL
                     VGANYIPASAINELEMWQAATFDPQQIDKELAWAEGLGMNTMRVFLHDLLWQQDAMGF
                     KQRLDQFLAIAAKHHIRPVLVLFDSCWDPLPKLGKQHAPTPGVHNSGWVQSPGAKALK
                     DASQYPRLKTYVQGVVGAFADDPRILAWDVWNEPGGFQYGYYEKHEPKNKVELVKALL
                     PQAFAWARSANPTQPLTSGLWQGDWPKSGPTPMEVIQLQNSDIITFHNYSWPEDFERR
                     VVWLEKQHRPIICTEYMARSAGSTFDGVLPIAKKHNVGAINWGLVVGKTQTNFPWESW
                     QHPYVLEPPPVWFHDVFKADGTPYRETEAQLIRQLTGKTAAVASH"
     gene            8155..8595
                     /locus_tag="VFC15_17430"
     CDS             8155..8595
                     /locus_tag="VFC15_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11994.1"
                     /translation="MKKFWIAVVGTALMFGPALYAQDVVTGVKDLGKDVDKGAKKTGD
                     ATVDASKATAKGTKKAAVATGDATKDATKATVHGTAKAADKTADATKDVSKDVAHGTK
                     KAAVKTSDATVDASKATAKGTKKAAVATGDATKKAADKTADAVK"
     gene            9068..10033
                     /locus_tag="VFC15_17435"
     CDS             9068..10033
                     /locus_tag="VFC15_17435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZM11995.1"
                     /translation="MDFDQLTTFLEVVKLGNFSRAGEKVFRSQSAVSAQIRQLEQEYG
                     TKLLDRSGKRVTLTPAGEVLFEYANRLLALRGESLRAVVDQDNTPRGVLAIGANEATC
                     LYVLPETFSEYRRRYSDVQISIYRNFSHKILERLQDGLIDVGIVTMPVKASRLVVKRI
                     FHDRLVLMTGPDHPLGQLDSVSVEAMVDYPIILPKAGHTRKTMDKLFRPYQSQLRIAM
                     ELPSIAMIKAFVASGMGISLISESFARKECRAGDLRLVPLSDTDLSRELALVYHQDRT
                     LPRSAMAFIEMVRNGLPRFRSAGAFEIEGTRLMESHELDANFLEF"
     gene            10397..11641
                     /locus_tag="VFC15_17440"
     CDS             10397..11641
                     /locus_tag="VFC15_17440"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM11996.1"
                     /translation="MRLVSRACTALLGVSLAVFSASPGVAQDAPAPKPADTPQASTPA
                     PAPGAPAAPADAPTALPTPSVTGPLAAAVPITLEAGPLGKLSLNGIVSGFGVFQSNSV
                     PGNSGAEGNLSNGQIWIQKTTSWWQFYVQAGAYDITALGSPYISTETAMTDLYGPVPT
                     AYFKLVPGKTTSIEFGILPTLVGAEYTFSFQNMNIERGLLWNQENAITRGIQINQVLG
                     KYFTASFSWNDGYYSNRYSGLSGSLTYTKGPHSLAFVGAGYLGQTNWTNLATPIQNNG
                     QIYNLIYTYTKNGWVIQPYVQFNVVPTNPAIGIPQGAQAWGGAILACKTLKKGWSMAG
                     RFEYISSNGSVASNSVNLMYGPGSAAWSLTATPTYQYQKFFTRADISFAKATSMTPGF
                     GFGSLGTISSQTRGVVEIGFLF"
     gene            11790..12128
                     /locus_tag="VFC15_17445"
     CDS             11790..12128
                     /locus_tag="VFC15_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZM11997.1"
                     /translation="MTKVEAIIQPSRFEAVKDALIEIGVEGMTVLEVRGHGRQKGHTE
                     SYRGREYSVDLLPKIKLELVLDDKLAAQAVETILKVASTGKIGDGKIFLSKIDDAIRI
                     RNQERGVSAL"
     gene            12346..12792
                     /locus_tag="VFC15_17450"
     CDS             12346..12792
                     /locus_tag="VFC15_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HZM11998.1"
                     /translation="MSSFVQQLMLRLINIWPPIAGAGIRVRWSADRKSVDVEMKLRFW
                     NRNYVGTHYGGSLYSMTDPFYMLMLMNNLGPGYIVWDKAAAIRFRKPGKGKVRAEFRL
                     TDTQLDDIREQLTALPKYEPVFKVEVKDEAGEVVAEVEKVLHIRKK"
     gene            complement(12882..14348)
                     /locus_tag="VFC15_17455"
     CDS             complement(12882..14348)
                     /locus_tag="VFC15_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM11999.1"
                     /translation="MKAFARLAIALTVLLALAGLASAQQWAPLNNQPGAAVGPMLQLR
                     DGRILVNESQGPDTGVWFILTPDATGSYANGTWSTTGHLPAGYAPIYFGSQVLLNGKQ
                     VVIEGGEYNNGTAVWTTLGAVGNIVPFGNVTWTLNSPPSGWGTIGDAESITLANGHYM
                     QANCCTPQNAIFQGPNSWLATGSVSQSSNDESGFTLLTTDKVLTVDAKNSPCGNGVNG
                     GAELYNQGTGAWSCTGQTPVHLYNPADEELGAAVLMYNNKVFQLGGNIVASAVYDVAS
                     GTWAAGPTPPNGLDQADGPSALEPNGKVLSMLSPGLFQGGCQFLEYDPTAGTLANAAN
                     PTNCPSDSSFVGHLMILPTGQLMFTDFSGLVELYTPASGVVAAAKPTILSASTNLKIG
                     STNNILYGSQLNGLTQNNGYGDDYSGDTDYPLVRLTSTSSGKVYYAFTHDDSTHSIKP
                     GTVMYTFFDLPSTIPAGTYSLQSVANGIGSNSVIVSVH"
     gene            complement(14498..15181)
                     /locus_tag="VFC15_17460"
     CDS             complement(14498..15181)
                     /locus_tag="VFC15_17460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM12000.1"
                     /translation="MSHRLTRKFTLAAMAALLLILAVPGWAGQVQVYAQGPNYNGLYA
                     SQNDTAGFGLFAQSYDNFSLGSATTITSASWVGGYFNPQSAGSITGWTVSFYANSAGQ
                     PGGLISSSTFSGNGGETFLQNDSLGDPVYSYTSGSLSFAAAAGTTYWLSVVPDVAFPP
                     QWGWTTSSTGDGVAWQVFFGTGSSIPSDLAFSLYKTQQTTVPEPGSLMLLGTGIVGLA
                     GVIRRKLGA"
BASE COUNT         3435 a         4416 c         4558 g         3128 t
ORIGIN      
        1 cgatccagcg gcgcgcctgc gattgttcga tacgcgggcc gagtggcggc aagtcgcggc
       61 gggggccgtc aggaacgaag tgcagcgtct ccagcgagtc accttcccag ggatcgggat
      121 cgtcgtcgcg caacgcgggc agcatgtcgg aggcttccac cggatgcagc ggctgtatgg
      181 tccccaagtc gatgaggcgc gagttctcac gggtctcgca gaattgcttc cacgccgtca
      241 gcgcctgttc gacggtatcg atgccgagag tggcggcgaa atcgttggcg ctgcccatgg
      301 gtacggccag cgtgggcgtg cgcttcaata ccagcgttcc gaggtggcga tgcaccgttc
      361 catcgccgcc aaagatcagc gcggcatcgg gttcgtcggt gggatcgagt tcgttgcctg
      421 tgaaaatgct gacgcccggc aattcaaatg ggcggacgtc ttgctgtttc gcgaacggtg
      481 acagcatggc aacagcgcgc accgggcaag ggtcgcataa cgcgccggaa aatcaaaatc
      541 cgggggatga aaccgattgc cggattccga agtgcgcgat aacaaaccaa atccagttct
      601 tgacattaca gattgcaata ttataattgc taatcgtaat atgagactcg gcgaaaaact
      661 ccggtacctg cgcctggtgg aaggcacgtt gcgcggcatg gaacgcgaaa tgacgcagca
      721 tgagctcgcc gtagcactta agaaagaact gggcaggggt ttgagccagt cctatctgtc
      781 gcagatcgag agcggagcgc ggccgcacct gaccaacgcg tctcgcatgt tactggcgcg
      841 gtacttcaat gtgcatccgg gatacctggt cgacgatccc gagggctatc acacggagtt
      901 gaccagcgac ctgcgcgccc gggaagaccg gctcgacctt tggcttatca atggcgcgga
      961 gcagttcgca cgcgatcccg agttgagcca ggcgctgatc gaggtggcca aatacaagga
     1021 ctcgcgcaag tgcctggttc tgttgggtgc gattctggaa gcgccggagc tggtggagcg
     1081 cttgacggaa gttctgaagc cgaatctgag ccagcctctg catcgcggat ccgggaagag
     1141 gagatcggcg ggagcgaagc tatgacgtgg gcgatgttct atctcgtttg ctttctggtg
     1201 ggagtgacgc tcagcgcgtt gtcgttcctc ggcggaagtt ttcacctgcc ccacttccat
     1261 gttcatgtgc cgcatgttca cgtgccacac ggtggcgcgc acgtcggcac cggcggcgag
     1321 atgccgttcc tgaacttcgg gacggtcacc gcgttcctgg cgtggtttgg cggatcggga
     1381 tacctgctca cacgctactc atcgttcatg gtcgcgggag tgttgacgct ggcggtcatc
     1441 gctggactcg tgggcgcgat gatcgtcttc tggttcgtgg ccaagctgct gctcaagcac
     1501 gatcgcgaac tcgatccggc ggactacgag cgcgtgggag tcctggcgcg catcagcagc
     1561 ccggttcgtg agggcggcac cggggagatc atcttttcgc aagaaggtac gcggaacacc
     1621 tgcggcgcac gcagcgaaaa cagcgaggcc ctgccgcgcg gcaccgaggt gatcgtcacg
     1681 cgctacgagc acggcatcgc ttatgtgcgg cgctgggaag agttggctga gaaagacgca
     1741 gccagcagct aagttttcaa ggaggcagta tggggtttca tattccgaca gaagtactaa
     1801 tcgtcgcctg gaccgcggtc gcggctgtgg tgatgatcgt cgccgtattc gccaggctgt
     1861 accgcaaggc cgggcccaac gaggtcctgg tggtgtacgg atttcgcggc acccgcgtcg
     1921 tgaagggaca cggtacggtc atcttcccga tggttgagac ctaccgggag ctgtcgctgg
     1981 agctgatgtc gttcgacgtg gcgccacagc aggaccttta caccaagcag ggtgttgccg
     2041 tcacggtaga ggctgtagcc cagatcaaag tgaagtccga tccggaatca gtgctgacgg
     2101 cgtcagagca gttcctgact aagaagccgc cagaccggga agccttgatc cggctggtaa
     2161 tggaagggca tctgcgcggc atcatcgggc agctcacggt ggaagagatc gtgaagcaac
     2221 cagaaatggt cgctgatcgg atgcgttcca cctgtgccga cgacatgaac aagatgggac
     2281 tcgaggtgat ttcgttcacc atcaaggaag tgcgtgacaa gaacgagtac atctccaaca
     2341 tggggcggcc ggacgtggcg cgaattaagc gcgacgccga cgtcgccacc gccgaagcgg
     2401 aacgcgacac cgctatcagg cgcgccattg cgctgcggga atcggcgatc gccaaggcgc
     2461 aagccgacca ggagcgcgtc gcggctgaaa cggcttccct ggcgcgacag gctgaggcgc
     2521 aacgcgatct cgaagtgaag aaggccacct acaacgaatt ggtaaaacgc cagcaggcgc
     2581 aggccgacaa agcctacgag atccagacca atatcatgca gcagcaggtg accgccgagg
     2641 ctgtgaaggt ccagcaagtc gaaaaagagc agcaggtgaa ggtgcaagag gccgaaatcc
     2701 tgcgccgcga gaaggaactg atcgccaccg tgttgaaggg ggccgaaatc gagcgcaagc
     2761 ggattgagac gttggccgaa gcggaacggc agcgcttgac ggtggaagcg gaaggccatg
     2821 ccagcgcaat tcgcgcacag ggcgaagccg aagccgagat catcttcaag aaaggcgacg
     2881 cggaagcgcg ggcgatgaac ctgaaagccg ccgcctacca ggagtacaac caggccgccg
     2941 tgtacgacaa actcattacc ggtttgccgg aagtggtgaa agcgctggcc agcccgctga
     3001 acaacgtcga caagatcacc attgtttcaa ctggcaacgg tacgtctgcc ggcatgcaca
     3061 agattacggg tgacatcacc gagatggcag cgcaagtgcc ggcgctgttc gaggcgctgt
     3121 cgggcatgaa gatgtcggac ctgcttggga agatccggac gattggagag aagtcgccgc
     3181 ctgaactgcc gcccgcagga ggcgatggca gctccaattc ggcagggagc agcaagtgaa
     3241 atggcaaacg tgtccacccc gtcgcaaggg cgtctgcatg agtccgccgc gcgaagccga
     3301 cagggttggg cacccagatt ccagaggaaa gagctggatg cgcgctgaac gcacttcatg
     3361 aaattcagca aggagcagct atggcattac tcgatcgtgt agcaacgttg gtacgggcca
     3421 acctcaacga tctcgtggat cgggccgaaa accccgagaa gatgttgaag caggtcatcc
     3481 tggatatgga gaaccagttc attcaggtga agacacaggt ggccattgcg ctcgccgacc
     3541 ttcacctctt gaagaagaag aagcaagaaa acgccgacaa gcatgcagaa tggatgcgca
     3601 aggccgaact ggctgtcgaa aagaaggatg acgaactggc acgcgcggcc ttggagcgct
     3661 ctatgagctt ccaacaactc gcggaaagtt ttgaggagca gatctccgac caggaagccc
     3721 aggtcgaggc gctcaagtca gccctgaaga agttggaagt caagctctcc gaggcgcgcg
     3781 ccaaagtgga cctgttgatc gcgcagcatc gccgctcgcg tgcagtgaat cgggcagccg
     3841 acgcccagca gatgcccggc ggagaaaacc tcaccttcga ccgcatgcgc tccaaggtcg
     3901 cgcgcgagga agctctgggg aaagccaagg gtgagttgct gggcgacgac attgacggac
     3961 ggttccacgc actggagagg gagcagaaaa tcgatgctct tttggaggag attaaagcaa
     4021 aaaaacgcct gagcgcctga cttgacccgt tcgttttgcg agatgctgaa atcgtaagag
     4081 aaatcttgct ccccggcatg atttcgcacg tctaacactg gagagacggg cggctggagt
     4141 ttcgtcccga gaaactcgac ctccgtcgcg cggaggacgc atgaagcgca gagagttcat
     4201 cgtcaacact tccctgggac tcggagccgc atggctggct cgcaactcca ttttgaacgc
     4261 gcttgctgag acgcagcttc cgcgcaagta cagcgcgtcg gatacggtga ctctgggcaa
     4321 gaccggcatc aagaccagcc gtctcgccat gggaaccggc accgtcggtt acgcaaaaca
     4381 ttccaaccag accgcgctcg gagttgccgg actctcgggg ctgctgctca atggctacga
     4441 ccaaggactg cggttcttcg acgccgccga ttcgtatggc agtcatcccc atgtagcggc
     4501 cgcgttgaaa caacttcccc gcgacaaagt tacagtgatg accaagagcc cctcgcgcac
     4561 tccgcaggaa atgcgcgccg acctcgatcg ctatcgcaaa gagctgggca ccgactacct
     4621 cgacatcgtg ctgatgcact gcatgaccga aggcgattgg accgagcgct tccaaggcac
     4681 gatggatgtg ctgtcggaag caaagcagaa aggcatcatc cgcgctcacg gctgttcgtg
     4741 ccacactatc gaggcgctgc gcgccgccgc caagtcgccg tgggcggagg tcgacctggt
     4801 gcgcatcaat cccatcggct cgcacatgga cgacgatccc gccaccgtgg tcagcgtgat
     4861 gcagcagatg aagtcgtcgg gcaaagggct catcgggatg aaaatcctcg gccagggcga
     4921 cctgcgcacg cggcagagtg aagcgatcaa gtacgcgctg tctctaaatt tgctcgacgc
     4981 cttcaccatc ggcgcggaaa gcaagagcga gcaggacaat ttgatccagc ggatagccgc
     5041 agcgtagccg ggcgagacat tcacccacat tccggtgttg ttagtacccg gggttgtcaa
     5101 aacgaacgcc cgtttttggg cgtgaggaat ctgctttttc tccgaaggcg caaactgcgc
     5161 gacggagatg ttcgcccgaa ggagagttgc atgcagcaga agaccgtcaa gctaatcgct
     5221 ttagcattcc tcgtgcaggt tgtctcactt ggcgcgacct ttcaggcaca agcgcaagcg
     5281 gagaagactt cctatcccgc aatggctccg ctcgatcagt atttgatgcc cgaccggaat
     5341 tctgaaatcg cgctggctcg cactggtgcc ccggcctcca tctccgacgc agccgaagtc
     5401 atggtcctgg gtccgcaggg ctactccacc gctgctaagg gcaccaatgg ctttctgtgc
     5461 atcgtagaaa gatcatgggg cgcctccacc gacgatccga atttctggaa ccccaaaatc
     5521 cgtgccccca tttgcttcaa tgcgccagcc gtgaggacct ttgtgcccat ttttctgatg
     5581 aaaaccaaac tggtgctggc gggaaaatcc aagccagaga tcgtcgcggc gaccacatcg
     5641 gccttcgata agaaggaact acccgcgctc gatccgggag cgatgtgcta catgttgtcg
     5701 aagcagcagt atttagatga caaggccacg cactggcatc cccacctgat gttttttgtt
     5761 gcaggcgacg cagcgaaaag ctggggagcc gatctgccgg gctctccgat aatcgcggca
     5821 aatgaccctg aagaacgggc caccattttc atggtcgtgg tgggcaaatg gtcggatgga
     5881 tccccggctc cgcagcagat gcactgacct tcgactttag acagcagtcg aggctacgct
     5941 ttcggctagc gcgccgtttg cgtgttttct tcgtctggct ctagttctat gtccagtgac
     6001 ttaacctcgc cgcgttgaac acgaaccggg aacttttctg aggggttcga cggatcgacg
     6061 tagacccatt tcttgtagcc ggtagctgac acttcaatgg aaaggcctct gtccacagga
     6121 atacacgtac gggtcatcgc acgtccgaac ataatacttc ttccagcttc cctcgaaggg
     6181 tctgcgttct ccatctcgaa tttcaccctg aatttcttca ctggttcccc ggtgactttg
     6241 tcctttacgg aaggagccaa gataccttct tgttgaccaa gtttcgcaat gacagtgcgc
     6301 acagggtacc caggcgtaag tgtgacggtt tgtctttcgc gaccctcaaa ataacgtgga
     6361 tacccatctt cttccttcga cgcaatcaac aaaaaggttc ccatgggcag gtggtccacg
     6421 tgatattgac ccgtctcatc tgttattgcc agacaatttc cggtttttcc cgtattgata
     6481 aagaaaacat tgttgcatag cgcgtcctgg gtcggatcct tgtgttcaac aagcataaga
     6541 ccagcaacgt gtgctccgct gaccggttgg ttgtgttcat cgagcaccgt gccgacaatt
     6601 tgcccttcga gttgagtttg gccgccgact tgcgacaacg ctggcaatac aaaaaacaac
     6661 attagggtga ttgcaaacca agttgaacgc attaccgcgc ctcccctccg gactcataaa
     6721 acgaaatttc ctcgttagcc accagcccca tattatgccc cccactcgaa gatctgcttc
     6781 aataagtcct agtgagacgc gacagctgcg gtcttccccg tcaactgccg gatcagttgc
     6841 gcttcggtct cgcgatacgg cgtgccatcg gccttgaata cgtcatgaaa ccacaccggc
     6901 ggcggctcca gtacataagg atgctgccac gactcccagg ggaagtttgt ttgcgttttg
     6961 ccgacgacca atccccagtt gatcgcaccg acattgtgct tcttggcaat gggcagtacc
     7021 ccgtcaaagg tgctgccggc cgaacgcgcc atgtactccg tgcagataat cggacggtgc
     7081 tgcttttcca gccacaccac gcggcgctcg aagtcttccg gccaactgta gttgtggaag
     7141 gtgatgatat cggagttctg caactgaatg acttccatcg gcgtcggtcc cgatttcggc
     7201 cagtcgcctt gccatagtcc gctggtgagc ggctgggtag ggttcgccga gcgcgcccag
     7261 gcgaatgcct gcggcagcag cgccttcacc agttccacct tgttcttggg ctcgtgcttc
     7321 tcgtagtagc cgtactgaaa tccgcccggc tcgttccaaa catcccacgc caggatgcgg
     7381 ggatcgtcgg cgaacgcgcc cactactccc tgcacgtaag tcttcagccg cggatactgc
     7441 gacgcatctt tcaaggcttt ggctcccgga ctctgtaccc atccggagtt gtgcacacca
     7501 ggtgtgggcg cgtgctgctt gccgagtttg ggcagcgggt cccaacagga atcgaaaagc
     7561 accagcaccg ggcggatgtg atgcttcgcg gcgatggcga ggaactgatc gaggcgctgc
     7621 ttgaatccca tcgcatcctg ctgccacaac agatcgtgca gaaagacccg catggtgttc
     7681 atgccgagtc cctcggccca cgccagttcc ttgtcgatct gctgggggtc gaaggtagcc
     7741 gcctgccaca tctccagttc gttgatcgcg ctggccggaa tgtaattggc tcccaccagc
     7801 cagggttgct gctgatacca ggtgttggct ctctgctctg accagcgttc cgccgattgc
     7861 gccggcactt gcgccggcaa cccgagcaaa agtacgaaca gaattacaga tgagagcaag
     7921 cgatgcatga gcgtccccaa ttgttctgcg acagactact ccggattctt ggattcgcag
     7981 tgagttgttt gatcaatcta ggcgcgcgcc agcccagaat gttatcctta acgtaccttc
     8041 ccgataagag aataatgccc cttattggcc ggtgcttcca tccggcagac ggctatctta
     8101 tgacggagga atttctaagt ttcagtggct cgatcgagtc gggggagatt caccatgaag
     8161 aaattttgga ttgcagtcgt aggtaccgcc ttgatgtttg gtcccgctct gtatgcgcag
     8221 gacgtcgtca ccggcgtgaa ggacctgggt aaggatgtcg ataagggcgc gaagaagacc
     8281 ggcgatgcca cggttgacgc ttccaaagca accgccaagg gcaccaagaa ggccgctgtc
     8341 gctaccggcg acgcgaccaa ggatgcgacc aaggccactg tgcacggtac ggcgaaggct
     8401 gctgacaaga ccgctgacgc gaccaaggac gtcagcaagg acgtggctca cggaaccaag
     8461 aaagctgccg tgaagaccag cgacgccacc gttgacgcat cgaaggccac tgcgaaaggc
     8521 accaagaagg ctgcagtcgc aaccggcgac gcaaccaaga aggccgctga caagacggct
     8581 gacgcagtaa agtaacagct ttacatccca agggtgccgc cacgcgtgaa tccgacgcag
     8641 cggcgccttt tcttttgcca agaaccgccg ccgggcgaag cggcaggttt ggccccgcgc
     8701 ggcagcaact tcttcccggc gggattcacg ccgcacacgc gactccactg ctagcgatcg
     8761 caacttaact cttgcgggac aatccgtttt gcgtttcgat cacgcaatct tatgccctcg
     8821 ataaaaagta tcaatttcct caatcaccga gcggcttcta aacttgatca acaacctgag
     8881 ttagattgag tcgactttgc gttcctgatt gcgtattacg tgcggctctg ggcgcaggcg
     8941 ccgatcaagg atctccacca agccagcaac aagactggca ccagttttcc acagcggtgg
     9001 gtagcgactg ctgggttcag gcattttcgt tgtgtgctca caagaatgtt tcaggtggag
     9061 tgtctgcgtg gacttcgatc aacttacaac atttctggaa gttgtgaagc tcggtaattt
     9121 ttcccgcgcc ggggaaaagg tgttccgttc gcagtcggcg gtgagcgcgc aaatccgcca
     9181 gctcgagcag gagtatggca cgaaactgct ggaccggtcg ggtaaacggg tcacactcac
     9241 gcccgccggc gaagtgctgt tcgagtacgc gaatcggctg cttgccttgc gcggagaatc
     9301 cctgcgcgca gtggttgacc aggacaacac acctcgcggc gtgctggcga tcggtgccaa
     9361 tgaagccact tgtctctatg tgctaccgga aacgttcagc gagtatcgca ggcgatactc
     9421 cgatgtgcag atcagcattt atcgtaactt cagccacaag atacttgagc ggctgcagga
     9481 cgggctgatc gacgtcggca tcgtgaccat gcccgtaaaa gcttcgcgat tggtggtcaa
     9541 gcgcatcttt cacgaccgcc tggtgttgat gacgggcccc gatcacccgc tgggacaact
     9601 ggattcggtt tccgtcgaag ccatggtgga ctacccgatc atccttccca aggcgggaca
     9661 cacccgcaag acgatggata aactcttccg cccttaccag tcacagttgc ggatcgccat
     9721 ggaattgccc agcatcgcaa tgatcaaggc attcgtcgca tcgggcatgg gcatctcgct
     9781 gatcagcgag agcttcgcgc gcaaggaatg ccgcgccggc gatctccgcc tggttccctt
     9841 gtcggacaca gacctgtcgc gcgaactcgc attggtgtac caccaggacc gcaccctgcc
     9901 gcgttcggcg atggcgttca tcgagatggt acggaacgga cttccacgat tcaggtccgc
     9961 tggcgcgttc gagatcgaag gcaccaggct gatggagtcg catgagttgg atgctaactt
    10021 cctcgaattt tgagcaaagg actctccagt tctcgtaata attgtctgca ccaactgttg
    10081 cggcactcta tccccgcgga atgcacgcgg tcgaaactcg tatagtcttg cgccccaccc
    10141 aataatagat attcctgcat atcgctgatc ctacataggt gctaccagta ttgactgagt
    10201 agactcgtat cccctgataa gcgttgctta tcgtcctcat aaaaaactgc tgtcgcagaa
    10261 atttcgattg gcctcaacgg aactatgcgt tggactgaca gcgtacggtt cgttagcttc
    10321 agagtctcta gtcaacgaac cgctgactca tcaaacggat gagcgcggga cactcagaag
    10381 aaagcgagcc ttccagatga gacttgtttc ccgagcgtgt acggcactac tgggcgtgag
    10441 ccttgcggta ttttccgctt ctccaggggt ggcacaggac gcgccagctc ccaagccagc
    10501 cgacacaccg caagcgtcga ctccagcccc agcgcccggc gcgcccgctg ctccagccga
    10561 tgctccgacg gcactcccta cgcctagcgt tacaggtccg ctggctgcgg cggtgccgat
    10621 cactttggaa gctgggcccc tggggaagct cagcctgaac ggcattgtta gcggctttgg
    10681 cgtgtttcaa agcaactcgg ttccgggcaa cagcggggcc gaaggcaacc tgtcgaatgg
    10741 acagatatgg atacagaaga cgaccagctg gtggcagttc tacgtgcagg ctggcgccta
    10801 tgacatcact gccctgggat cgccttacat ctccaccgag accgcgatga ccgacctcta
    10861 cgggcctgta ccgacggcct acttcaagct cgtaccgggg aagactacct ccatcgagtt
    10921 tggaattctg ccaacattgg taggcgccga gtacaccttc agctttcaaa acatgaacat
    10981 cgaacgtggc ctgttgtgga accaggaaaa cgccatcact cgcgggatcc agatcaatca
    11041 ggtgttggga aaatacttca cggcctcgtt tagctggaac gatgggtatt attccaaccg
    11101 ctattcggga ctgagcggat ccctgaccta caccaaggga ccgcacagct tggcgttcgt
    11161 tggcgccgga tatctcgggc aaaccaactg gaccaacctg gctacgccca ttcagaacaa
    11221 cggccaaatc tacaacctga tttacaccta cacgaaaaat ggctgggtca tacagcccta
    11281 cgttcaattc aacgtagtgc ccaccaatcc cgcgatcggc atcccgcagg gcgctcaggc
    11341 ctggggcggc gcgatcctcg cctgtaagac cctcaagaaa ggctggtcga tggcaggccg
    11401 ctttgaatac atatcgagta atggaagcgt cgcttccaac tccgtcaacc tcatgtacgg
    11461 ccccggcagc gcggcctggt cgctcacggc gactcccacg taccagtatc agaagttctt
    11521 cacccgcgcc gatatttcat tcgcaaaagc caccagcatg acgccgggat ttggtttcgg
    11581 ttccctcggt accatcagca gccagacccg gggcgtggtc gagataggat tcctgttcta
    11641 gagcagtgga cttagcacag gcccctgtgt gaccgtatga gaagatggaa cacgtttggg
    11701 catctcgatg gcgaatcggc atcctgctct ctcgaatgcg agcgcgagca ggatgccgat
    11761 agccgcatac gcgattggga ggacggggca tgacgaaagt tgaagcaatc attcaaccct
    11821 cgaggtttga ggcggtaaag gacgccctga tcgaaatcgg ggtggaaggc atgaccgtgc
    11881 tggaagtgcg tggccacggc cgccagaagg ggcacaccga gtcgtatcgc ggccgcgagt
    11941 acagcgtaga cctgctgccg aagatcaagc tcgagttggt cctggatgat aagctcgcgg
    12001 cgcaggccgt ggagaccatc ctgaaagttg catcgaccgg caagatcggt gacggcaaga
    12061 tcttcttgtc gaagatcgac gacgcgattc gcatccgcaa tcaggagcgc ggcgtttccg
    12121 cgttgtaaag tctgggcttg attcgagaac tcgccgctcc gaatctgaga ataggcccaa
    12181 cagacttccg tgaaatcagt agccggggcc gtacgctccg gctgctttta ttttgggaca
    12241 gcgtccagag agatttgatc gccacaactg gacgacatgg ctgtcgagtc tcaagctcca
    12301 ggcttgtaaa ctggtgtttc ggcaacaagg aagccacatc tctcaatgtc atctttcgtg
    12361 caacaactca tgctgcgcct gatcaacata tggcctccga tagcgggcgc gggcatacgc
    12421 gtgcgctgga gcgccgacag gaaatcggtc gacgtggaaa tgaaactgcg attctggaac
    12481 cgcaactacg tgggcacgca ctatggcgga tcgctgtact ccatgaccga tcccttctac
    12541 atgctgatgc tcatgaacaa tctcggcccc gggtacatcg tttgggacaa ggcggccgcg
    12601 atccggttcc gcaagcccgg caagggcaaa gttcgcgcgg agtttcgact caccgatacg
    12661 caactcgacg acattcgcga acagttgacc gcccttccga agtacgagcc tgtctttaag
    12721 gttgaagtga aagacgaagc aggtgaggtc gttgccgagg tggagaaagt tctgcatata
    12781 cggaagaagt agccatcctc acttccgtgc ccgaataaaa aagtgccatg ctggtgggag
    12841 catggcacgt gtagtttcag agttactgag ttgcaaaaaa cttagtgcac cgacacaata
    12901 accgagttcg atccgatgcc attggcgacg gactgtaacg agtacgtccc cgctggaatg
    12961 gtcgaaggca gatcgaagaa cgtgtacatg acggtgccgg gcttgatgct atgcgtactg
    13021 tcgtcgtgcg tgaacgcata gtaaactttc ccgctggaag tgctggtcaa gcgaaccaac
    13081 ggatagtcgg tgtcgccaga atagtcgtcg ccgtacccgt tgttctgcgt caggccgttc
    13141 aactggctgc cgtagagaat gttattggtt gagccgatct tcagattggt ggatgccgac
    13201 aggatggtgg gttttgccgc cgcgacgaca cccgaagcgg gtgtgtagag ctcgacgaga
    13261 ccgctgaagt cggtaaacat gagctggccc gtaggcagga tcatgaggtg tccaacaaac
    13321 gacgagtcgc tagggcagtt cgttgggttc gcagcattgg ctagcgtacc agcagtggga
    13381 tcgtactcca agaactggca gccaccctgg aacaagccag ggctgagcat cgacagcacc
    13441 ttgccgttcg gttccagcgc cgatgggccg tccgcctggt ccaatccgtt gggaggcgtg
    13501 ggcccggccg cccaggtacc ggaagctacg tcgtaaaccg cgctggcaac gatattgcca
    13561 cccagctgaa agaccttgtt gttgtacatc agaaccgcag cgcctagctc ctcatccgca
    13621 gggttataca gatgaaccgg agtctgtccc gtgcacgacc aggcgcccgt accttggttg
    13681 taaagctccg caccaccatt gacgccgttg ccgcaagggc tgtttttcgc atctacggtt
    13741 agaactttgt cagtggtcag cagtgtaaat ccggattcat cgttgctcga ctgactaacc
    13801 gagccggtcg cgagccagga attcgggcct tggaagatcg cattctgcgg cgtacagcag
    13861 ttcgcctgca tgtaatgtcc attggctagc gtgatgctct cggcatcgcc gatggtaccc
    13921 cagccggagg gcgggctgtt cagcgtccat gtgacattgc cgaaaggcac gatgtttccg
    13981 accgcaccga gcgtcgtcca cactgcggtg ccgttgttgt attcaccacc ctcgatcacg
    14041 acctgcttgc cgttcagcag aacttgcgag ccgaaataga taggtgcata gccggcaggc
    14101 aggtgtccag tcgtgctcca tgtgccgttg gcgtagctgc cggtggcgtc aggcgtcagg
    14161 atgaaccaga caccggtatc aggaccctgg gattcgttga ccaggatcct gccatcccga
    14221 agctgcagca tggggccgac ggcagcgccg ggctggttgt tcagcggtgc ccactgttgt
    14281 gcactggcga gtccggccag ggctagtagc accgtaagcg cgattgctag tcttgcgaat
    14341 gccttcatcg ttgtttctcc tctgagtaac cggtcaaccg ggcaggattt gcggcacacg
    14401 aaggcgcaat ttccgccaca gggagcggtt ctccgcaata tctagtttgg tttacttggc
    14461 cgcaggaacg gttcgtcccg cggccgcctc tccgacgtta cgccccgagc ttgcggcgaa
    14521 ttacgcccgc aagaccgaca attccagtcc ccagcagcat caggctgccc ggctcaggaa
    14581 cagtagtctg ctgcgtcttg tacagcgaaa acgccagatc gctcggaatc gacgatccag
    14641 ttccaaagaa aacttgccac gccacgccat cgccagtcga agaagttgtc catccccact
    14701 gcggcgggaa ggcaacatct ggcacgacgg acagccagta cgtagtaccg gcggctgcgg
    14761 cgaagctcag cgatccagag gtgtaggagt agaccggatc tcccaagctg tcgttctgca
    14821 ggaaggtctc accgccgtta ccagagaatg tagagctgga aattagcccg ccgggttgac
    14881 cggcgctgtt cgcataaaag ctaacggtcc agccggtgat ggagccagcg ctctgcgggt
    14941 tgaagtaccc tcccacccaa ctcgctgagg tgatggtggt cgcggatcct agactgaaat
    15001 tgtcgtagga ctgagcgaac aacccgaagc ccgcggtatc gttttgcgaa gcgtaaaggc
    15061 cgttatagtt gggtccctgt gcataaacct gaacctgccc cgcccagcca ggtacggcca
    15121 ggattaggag cagcgctgcc attgcagcca acgtaaactt gcgtgtaagg cgatgtgaca
    15181 tttttcctcc tgcggaaatt cgaagccaac ttaaattgtg ttgatgctga gaagtgagca
    15241 ctgcgacaaa ctccaacccc tgagagctgc cgcggattct ttcttgcacg attatctcca
    15301 tattaaattt acactatgta attgatttca agttagttag cacactagca ttaaagtggt
    15361 gtgtgcagaa tattgccgtt tagtggactc tgcctccact actacggcac taggattaga
    15421 gcaatgtgga tcagactgaa ccctagtttt cgtgcttaat caagatattg gtggggtgaa
    15481 gtctcctcag tggtgaaccg acagccagca gcgagcctca gtttatactg cttatac
//
LOCUS       DASRKK010000721         9470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_856234, whole genome shotgun sequence.
ACCESSION   DASRKK010000721 DASRKK010000000
VERSION     DASRKK010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856234"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="VFC15_17465"
     CDS             complement(<1..2093)
                     /locus_tag="VFC15_17465"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZM12001.1"
                     /translation="MKLALALFLLCLFLIFAAWAFAQSATQHAPDVVTTFSALPNGVE
                     VTMGKTVLRVTAISGDVIRFRYAPTGVFGPDESFAVMKDTGFTAPAVKVTDKGPVVVA
                     QTGELSVVVNKSSLAVSIYDTAGRMIVTDRSDLPFTFTPEGFQTWKRMPDDEHYYGLG
                     DKAVSEDRREHGFSMWNTDSVMWEESTDPLYKSIPFFMGVRQGKAYGIFFDNTYRSNF
                     QFGKFSDSFYSFGAPGGELNYYFINGPQPKRVVGTYSALTGRTPLPPLFTLGYQQCRW
                     SYMTEARVREVTSGFRKRNIPADVIYLDIDYQDRNRPFTVNHQSFPTFEKMIKDLGQE
                     GWKTVLITDLHIAAVPGYKPFDEGVKGGYFVKNPDGTNYVGPVWPGPSVFPDFTRSEV
                     RKWWGSLYADFVKMGVRGFWNDMNEPSIFFRPDKTMPLDVVSSVEGRKTDQREVHNVF
                     GMENVHATYEGLLALAPDVRPFVLTRAAYAGAQRYAATWTGDNQSTWNHMRLSLPTVT
                     GLGVSGYPFVGVDVGGFSGSPTPELLTRWTALGTFLPIDRNHGSKGTRDREPWVDGPE
                     HEAIRKKFIEERYRLLPYIYTSMEETTRTGVPLMRLMVLDYPDDERMTTREAEGQYMF
                     GNNLLVAPKIKEFVDGYDMLVPEGTWYDYWTGQRVELKSQDDKEMKGKLHLNPKLDEI
                     PVFVRAGSIIPRQPLV"
     gene            complement(2233..2814)
                     /locus_tag="VFC15_17470"
     CDS             complement(2233..2814)
                     /locus_tag="VFC15_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12002.1"
                     /translation="MNRFVFFVSLLAAQFALAAACLAQSANGPISFQPGSDLQKRVLS
                     PSPAAGATKQSKQATIGRGQTMIDTGDAKNSFWNEAADLDGAGNIANADMLWDGSSKI
                     LYAFAHTNLRCAHGRSTEGDILIGVYGKKNVLGKTAGSGWWVVELALNQCQAPLPGLY
                     GCKFDAQGNAMACGRAELDTRINDMAIVESTKF"
     gene            complement(2843..3382)
                     /locus_tag="VFC15_17475"
     CDS             complement(2843..3382)
                     /locus_tag="VFC15_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12003.1"
                     /translation="MSLFAWGQSSHRPQSTAPKPEAAPTGAINPELETMTRAAAIQAR
                     PDQVGYFRSAIASTDTALQQSRELQTLGPDAQSTPIVNAKSLQLRDAIDDVEHYYGRL
                     IASFSRIQSTELKPLTKRLRKSYTFVAKEYKTVQQRMEPGKVVPERLTSGAANLEKAL
                     SDFRTDQVRLAREMGIQSK"
     gene            3397..3528
                     /locus_tag="VFC15_17480"
     CDS             3397..3528
                     /locus_tag="VFC15_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12004.1"
                     /translation="MRQQSLGAKSATQGFPMQEMMKKDVSSLRSTAAGEEAFRQHVD"
     gene            complement(3595..4485)
                     /gene="rsmI"
                     /locus_tag="VFC15_17485"
     CDS             complement(3595..4485)
                     /gene="rsmI"
                     /locus_tag="VFC15_17485"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZM12005.1"
                     /translation="MAKAKTTVRVVQNGVAPGSGTLYLVGTPIGNLEDITLRALRILK
                     EVDLIACEDTRHTVQLLDHYGIEKMTVSYHEHNELTRAAELIVHLEQGDNIAMVSDAG
                     MPGVSDPGYRLIALAVRHHIRIVPIPGASAFLSALVASGLPTDSFRFSGFLPAKEGAR
                     RTLLEGIRHSPRTQIFYEAPHRIVDALKDIVELLGPERHVVVAREVTKLHEEFLRGHA
                     EDVLDILEKRGDVKGEITLLIGKGEDTDHEQLSPKNVGQRVRELMSSDSLDEKAALKQ
                     AAKEFRVSKSEAYRELQRVK"
     gene            4597..5115
                     /locus_tag="VFC15_17490"
     CDS             4597..5115
                     /locus_tag="VFC15_17490"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HZM12006.1"
                     /translation="MAKIFAGRFTAAIDEPFVVFLIGMRINRLWAVHKWMPVAKAMPM
                     MLAMLEKNPAKGLLGVHSWVRWREVMAVQYWRSFEDLENFARNPTDPHLQTWKDFNQR
                     IGSDGSVGIWHETYLVNARQYECVYGNMPLFGLAAATNHVAAVGNRETARLRMGGQSE
                     PAVASPPQPPEA"
     gene            5259..6782
                     /locus_tag="VFC15_17495"
     CDS             5259..6782
                     /locus_tag="VFC15_17495"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZM12007.1"
                     /translation="MSPRAKSVAAKSKAEKTTASKSVRTITTTASTSSGYGVVDGVRT
                     PGDRAGTFDLRNANNTWEKRRDIGKGQRERAPRESHADTKPVANRPDPLDLLAASNVG
                     RQEDLIPLRMGRMAASPFTFLRGAACVMAWDLSKTPASGIHVIMCGDAHINNFGLYGT
                     PQRDVIFDLNDFDEITIGPWEWDLKRLVASVNVAARENGLNKNERAEAVMRAVEGYRF
                     NITRLQNMPVLDVWYLHAYPDRKNSLIQIDAKSSAVIAKCVAKAKRQTNATLLTKISE
                     RGVSGGWRLREDPPVLTHVDQATREKIIDGLNEYAESLPLERRYMLSRYHVVDVAHRI
                     VGVGSVGTRAYLALLFGNGDDDPLFLQIKECVSPAHAPYVPPLPKMYREHEGNRVVVG
                     QRALQASSDIMLGVTRVDGRPYFVRQMKNMKASVPVEWLTGESFNFYALACGTLLARA
                     HARAGDAAAIAGYCGGSAVLDKALATWAEGYGDQTEQDHDRLVKAIKSGKVKAIMGV"
     gene            6981..8171
                     /locus_tag="VFC15_17500"
     CDS             6981..8171
                     /locus_tag="VFC15_17500"
                     /inference="COORDINATES: protein motif:HMM:NF006580.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM12008.1"
                     /translation="MSNHKQRMPFSRRSFLQMSAVASAALAGRIVTEPMLAYAQLKSP
                     PKGGVRLNANENPLGPAAVARQAVANIMPDGGRYLFDLTEELINTFAQQQGLKPEYIL
                     AFAGSSPALHYAVAAFASPKASYVTADPGYEAGSHTAEGVGARVVNVPLTKTWSHDVR
                     AMLAAAPDAGIFYICSPNNPTGTLTSHADIEYLLEHKPAGSVVMVDEAYIHFCDAPTT
                     LDLVKADKDIIVLRTFSKLYGMAGLRCGFAVGRPDLLKKIEAYGGWNAMPVTASSAAI
                     ASLNAASLITERKRINTANRESTFAWLDSNGYKYSPSVSNCFMVNARKPAGDVIAAMA
                     HQNVYIGRPWSSMPDWVRVTVGTQAEMNQFQTAFQAVMKGAIVGRVYDERLHRNLDGI
                     MVTG"
     gene            complement(8396..>9470)
                     /locus_tag="VFC15_17505"
     CDS             complement(8396..>9470)
                     /locus_tag="VFC15_17505"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZM12009.1"
                     /translation="GDRLSFRVVRDVDVQGFTVIPAGSTASGSLARVHGRRVLGMGGN
                     LVLKLDSVELANGDRVPLRARKEVKGTSHTWRMAAGMAITSIFYMPAAPVFLLTRGDN
                     CTALKSTEITAQINGTTSLAAADFPVTTASTSDLNQVTQFLPPRVLDGDGRQGDMVNL
                     LFIGHKEDVQSAFEDAGWVKPERWKLKSAWHVVTQRTHYVRQPMSRFFLFGRTQDYAY
                     ALPDPNAIVSRRHHIRIWKTGHEVDGNPVWAGAATHDVAIEVAKRGRLFNHRIDPNVD
                     SERDFIGAKLSQTSVTAHESYVEGAEPVFKAKTASGQDYYSDSKILLVDLRHPDTGEA
                     TLHNPARVGGGALTTSSLLAAAR"
BASE COUNT         1898 a         2808 c         2859 g         1905 t
ORIGIN      
        1 actaacggct ggcgcgggat gatcgatcca gcccgcacga acaccggaat ctcatccagc
       61 ttgggattga gatgcagctt gcccttcatc tctttgtcgt cctggctttt cagttcgacc
      121 cgctgccctg tccagtagtc gtaccacgtg ccttccggca ccagcatgtc atagccatct
      181 acgaactctt tgatcttggg agccaccagc aggttattgc cgaacatgta ctgcccttcg
      241 gcttcgcgcg tggtcatacg ctcgtcatcc ggatagtcca gcaccattaa ccgcatgagg
      301 ggaacacccg tgcgcgtggt ctcttccatg ctggtgtaga tgtaaggcaa caaccgataa
      361 cgctcctcga tgaatttctt gcggatggct tcatgctccg gcccatcgac ccagggctcg
      421 cgatcacgcg ttcccttcga cccatggttg cgatcgatgg gaagaaatgt ccccagcgcc
      481 gtccaccgcg tcaacaattc cggcgttggc gaacccgaaa atccgcctac atcgacgccc
      541 acaaacggat aacccgacac tcccagtccc gttaccgtcg gcagcgacag gcgcatgtgg
      601 ttccatgtgg attggttgtc gcccgtccat gtagcagcgt agcgctgcgc gcccgcataa
      661 gccgctcgcg tcagcacaaa cggacgcaca tcgggcgcca gcgccagcaa gccttcgtag
      721 gtagcgtgga cattctccat tccaaacacg ttgtggactt cgcgctggtc ggtcttgcgc
      781 ccctcgaccg aactcaccac atccagcggc attgtcttgt cgggcctgaa gaaaatcgac
      841 ggctcgttca tgtcgttcca gaacccgcga acgcccattt tcacgaagtc ggcatagagc
      901 gatccccacc acttccgcac ttcggagcgg gtgaaatctg gaaacacgct cggccccggc
      961 catactggtc ccacataatt cgtgccatcg ggattcttga cgaaatagcc gcccttcacg
     1021 ccttcatcga acggcttgta gcccggcacc gccgcgatgt gcaggtcggt aatcagcacc
     1081 gtcttccaac cttcctggcc gaggtcctta atcatcttct cgaaggtcgg aaagctctgg
     1141 tggttcaccg tgaatggacg gttgcggtcc tggtagtcga tgtcgagata gatgacgtcc
     1201 gccgggatat tccgtttgcg gaatccactc gtcacctcgc gaacccgcgc ttccgtcatg
     1261 tagctccatc gacactgctg atagcccagc gtaaatagcg gtggcaatgg ggtgcgccct
     1321 gtcaaagccg agtaggtgcc aacgactctc ttgggctgcg ggccattaat aaagtaataa
     1381 ttcagctccc cgcctggtgc gccgaaggaa tagaagctat cgctgaactt gccaaactgg
     1441 aaattgctgc gatatgtgtt gtcgaagaaa attccatacg ccttcccctg gcgcacgccc
     1501 atgaaaaacg gaatgctctt atagagagga tcggtcgact cctcccacat cacggaatcc
     1561 gtattccaca tcgagaagcc atgctcgcgc cggtcctcgg agacggcttt gtcgcccaat
     1621 ccgtaatagt gttcgtcgtc gggcatccgc ttccaggtct ggaagccctc gggagtaaac
     1681 gtgaacggaa gatcggaccg gtccgtcacg atcatccgcc cggccgtgtc gtaaatcgat
     1741 accgccagcg acgacttgtt gactacgacg ctcaactcac cggtctgcgc caccaccacc
     1801 ggacccttgt cggtgacctt caccgcagga gcggtaaatc ccgtatcctt catcacggca
     1861 aacgactcgt cgggtccgaa cacacccgtg ggcgcatagc ggaagcgtat gacgtcgcca
     1921 ctgatcgcag tgacccgcag caccgtcttc cccatcgtca cctcgacacc attgggcagc
     1981 gccgagaatg tcgtgacgac atcaggagcg tgctgagttg ctgattgggc gaatgcccac
     2041 gcggcaaaga tcagaaagag gcagagaaga aataaggcaa gtgcaagttt catgcggtgt
     2101 ccccgagacg actccgaagc gtaaagggga ttaaccagag tgtcaactgc gcgggggagg
     2161 gtgaatcggt tcgcgccgac gggaggcgcc gacctgtgag ctaccagctg cgagtagcgg
     2221 cttttgggtt tctcagaact tggtggactc gacgatggcc atgtcgttga tccgggtatc
     2281 caactcggcg cggccgcacg ccatcgcgtt gccctgggcg tcgaacttgc atccgtaaag
     2341 tccgggcagc ggagcctggc actggttcag cgccagctcg actacccacc atcccgaccc
     2401 tgccgtcttc cccagcacgt tcttcttccc atacacgccg atcaggatgt ctccctcggt
     2461 cgacctgcca tgggcgcacc gcaggttagt gtgcgcgaag gcgtagagaa tcttcgacga
     2521 cccatcccac agcatgtcgg cattcgcgat attacccgca ccgtccaggt ctgcggcttc
     2581 attccaaaac gaattcttgg catcgccggt gtcgatcatc gtctggcccc ggccaatcgt
     2641 cgcttgcttg gattgcttcg tcgcgcccgc cgccggtgat ggcgacagca ccctcttctg
     2701 cagatcggac ccaggctgga aactgattgg cccgttcgcc gattgcgcca ggcacgcggc
     2761 tgcgagagcg aattgcgccg ccagcagcga gacaaaaaag acaaatcgat tcatgcgttc
     2821 gcgcctatcc tcaaaaacca tatcacttcg actggatgcc catctcccgc gccaacctga
     2881 cctggtccgt gcgaaagtcc gagagcgcct tctccagatt cgcggcgccg ctcgtcagcc
     2941 gctcaggcac gaccttcccg ggctccatgc gttgctgcac cgtcttgtat tctttcgcga
     3001 cgaaagtata agacttgcgc aaccgtttcg tcagcggctt caattccgtg ctctgtattc
     3061 tagaaaacga agctatcagt cgcccatagt aatgctccac atcatcaatc gcatcgcgca
     3121 gttgcaggga tttggcattc acaatcggag tgctttgggc gtcgggaccg agggtttgca
     3181 actcccgcga ctgctgcagc gcggtgtcgg tgctcgcaat cgccgagcga aagtacccca
     3241 cctggtcggg cctggcctgg attgccgcag cacgcgtcat ggtctcgagt tcgggattga
     3301 tcgcgcccgt cggcgcagct tcaggtttcg gcgcagtgga ctgcggccgg tgggaagact
     3361 gtccccaggc aaagagagac attgcgaata acaagtatgc gacagcaatc actcggcgcc
     3421 aagagcgcaa cacagggctt ccccatgcag gagatgatga agaaagatgt tagcagcttg
     3481 cgctccacgg cggctgggga agaggcgttc cgacaacatg tggactgaga gtacgcgagt
     3541 ggatggcacc cgcggattgc tacacgcgag ctgccaattg ccagttatgc tcttctactt
     3601 cacccgctgc aactcgcggt aggcttcgct cttcgatacg cggaactctt tcgccgcctg
     3661 cttcagcgcg gctttttcgt cgagcgagtc ggaactcatc agttcgcgca cccgctgccc
     3721 gacattcttc gggctgagtt gctcgtggtc cgtgtcttcg cccttgccaa tcagcagcgt
     3781 gatctcgcct ttcacgtcgc cgcgtttctc gaggatgtcg agcacgtctt ccgcgtgtcc
     3841 gcgcagaaac tcctcgtgca gcttggtgac ttctcgtgcc accacgacgt gacgctctgg
     3901 ccccaacagc tcgacgatgt ctttcagtgc gtcaacaata cggtgcggcg cctcgtaaaa
     3961 gatctgggtg cgtggcgaat gacgaatgcc ctccagcaac gtccggcgcg cgccttcctt
     4021 cgcaggcagg aacccgctga agcggaacga gtcggtcggc agtccgctgg ccaccagcgc
     4081 cgacaagaat gccgacgctc ctggtatggg tacgatgcga atgtgatgcc gcaccgccag
     4141 cgcgatcaac cggtatccgg gatcgctcac ccccggcatg cccgcgtcac tcaccatggc
     4201 aatgttgtcg ccctgctcca gatggacgat cagctcagcc gcgcgcgtca gctcgttgtg
     4261 ctcgtgatag ctgacggtca tcttctcaat gccgtagtgg tcgagcaact gcaccgtgtg
     4321 ccgcgtgtcc tcgcaggcta tcaggtctac ctctttcagg atgcgcagcg cgcgcagcgt
     4381 gatgtcttcg aggttaccga tcggtgtgcc gaccaggtag agcgtccccg atccgggagc
     4441 gacgccgttc tgtaccacgc gcacggtggt ttttgccttt gccatacaac gcaagtctac
     4501 acccgcacga acgagcgcat gtcaaacatc caaggcgctg agtcaggact ctcgggcctc
     4561 caggcttgac tggcgtcaca ttataatcat tccgctatgg cgaagatttt tgcggggcgt
     4621 ttcactgcag ccatcgacga gccgttcgtg gtcttcctga ttggcatgcg cattaaccgg
     4681 ttgtgggcgg tccataaatg gatgccagtg gcgaaagcaa tgccgatgat gctggccatg
     4741 ttggagaaga atcccgccaa gggattgctg ggtgtccaca gctgggtgcg gtggcgcgaa
     4801 gtgatggcgg tgcagtactg gcgctcgttt gaagacctgg aaaattttgc gcggaatcct
     4861 accgatcctc acttgcaaac gtggaaggat ttcaaccagc ggattggttc ggatggcagc
     4921 gttggcatct ggcacgaaac gtatttggtc aatgcccggc agtatgagtg cgtttacggg
     4981 aacatgccgc tgttcggact ggcggcggca accaatcacg ttgctgcggt cggcaatcgc
     5041 gagacggcgc ggttgcggat gggcgggcag agcgagcctg cagtagcgag tccgccgcag
     5101 ccgccggaag cgtgaaacgc gccatttgat ggactgccgg cgacccgcct ctgttcacac
     5161 ccgtcacgct gcgttaatat tcgtagtccc caggtttacg ctgcggaagc tagcgacgca
     5221 gatttcctca tgccagctgg agaacgaaag gtttacctat gagtccacgc gccaaatcgg
     5281 ttgcggcaaa atcgaaggct gaaaagacca ctgcctccaa gtccgtgcgc acaataacga
     5341 caacagcttc gacgagcagc ggatacggcg ttgtcgatgg ggttcgaacg ccgggtgatc
     5401 gagcggggac atttgatctg cggaacgcca ataatacctg ggaaaagcgg cgcgacatcg
     5461 ggaaaggcca gcgcgaacgc gcgccgaggg agagccacgc cgacaccaaa ccggtggcca
     5521 accgtccgga cccgctcgac ctgctggccg ccagcaatgt gggacgccag gaggacctga
     5581 ttccgttgcg tatgggacgg atggcggctt cgccgtttac gtttctgcgg ggcgccgcgt
     5641 gcgtgatggc gtgggacctg tccaagaccc cggcctccgg cattcatgtg atcatgtgcg
     5701 gtgacgcgca catcaacaac ttcgggcttt acggtacgcc gcagcgcgac gtcatcttcg
     5761 acttaaacga ttttgatgag attacgatcg gcccgtggga gtgggattta aagcgcctgg
     5821 tggccagcgt gaacgtggcc gcgcgggaga acggactcaa caagaacgaa cgcgccgagg
     5881 cggtgatgcg cgctgtcgaa ggttatcgct ttaacatcac gcgtctgcag aatatgccgg
     5941 tgctggacgt ttggtacctg catgcctatc ccgaccgaaa gaattctctg atacagattg
     6001 atgccaagtc gagcgcggtg attgccaagt gtgtggctaa ggcgaagcgg cagaccaacg
     6061 cgacgctgct gacgaagatt tccgagcgcg gagtttctgg tggctggcgt ttgcgggaag
     6121 acccccctgt gctgacccac gtggaccagg cgacccgcga aaagatcatc gacgggctca
     6181 acgaatatgc cgaatcgtta ccgctggaac gccgctatat gcttagccgc taccacgtgg
     6241 ttgacgtggc tcaccgcatt gtcggcgtgg gcagcgtggg aacgcgcgcc tatctggcgt
     6301 tgctgttcgg caacggtgac gacgatccgc tgtttctgca aatcaaggag tgcgtatcgc
     6361 cagcccatgc accctacgtt ccgccgttgc ccaagatgta ccgcgagcat gagggcaatc
     6421 gcgtggtagt ggggcaacgg gcattgcagg cgtccagcga catcatgctg ggcgtcacca
     6481 gggtcgatgg gcggccgtat tttgtgcggc agatgaagaa catgaaggcg tccgttccgg
     6541 tggagtggct gacgggcgag tcgttcaact tctatgcctt ggcttgcgga accctgctgg
     6601 cgcgcgccca cgcgcgcgcg ggtgacgcgg ccgccatcgc ggggtattgc gggggatcgg
     6661 cggtactcga caaggcgctg gctacgtggg cggaaggtta cggcgaccag accgaacaag
     6721 accacgaccg actggtgaag gcgatcaagt cgggcaaggt gaaggcgatc atgggggtgt
     6781 agggggaagc ggtccagccg gagatcctac agcagtttca agcttcgatt ttcgagaaac
     6841 ccccgtccag ccaaacccag cttggacggg ggtagtcgct ttgctggttg ctgggctgag
     6901 attctggggt aagataaccc aaatcgggtc cgggtcagaa tcgtttccgg acgccgtttt
     6961 aggttcaggc gaggtggact atgtcgaacc ataaacagcg gatgccattc tctcgcaggt
     7021 cgtttctgca aatgtcggca gtggcttcgg cggcgctggc cgggcgcatc gtcaccgaac
     7081 ccatgctggc ctacgcgcag ttgaagtctc ctcccaaggg cggagtgcgg ctgaatgcca
     7141 acgagaaccc gctgggcccg gcggcggtgg cgcggcaggc cgtagctaat atcatgcccg
     7201 acggcggacg gtatctcttc gacctcaccg aggaactgat caacacgttc gcccagcaac
     7261 agggactaaa gccggaatac atattggcgt ttgcgggatc gagcccggcg ctgcactatg
     7321 ccgtggccgc gtttgcgtcg cccaaggcga gttatgtcac ggccgatccg ggatatgaag
     7381 ccggaagcca taccgccgaa ggggtaggcg cacgggtggt gaatgtcccg ctgactaaga
     7441 cgtggtcgca cgatgtgaga gctatgctgg ctgccgctcc cgacgccggt atcttctata
     7501 tatgttcgcc gaacaatccg acgggcacgc tcaccagcca cgcggacatc gagtacctgc
     7561 tggagcacaa gcccgcgggc tccgtggtga tggtggacga agcctatatt catttctgcg
     7621 atgcgcctac gacactagac ctggtgaagg cggacaaaga catcatcgtg ctgcggacat
     7681 tctccaaact gtacgggatg gcgggactac ggtgcggctt cgccgtcggg cgtccagacc
     7741 tcctcaagaa gatcgaggcc tacgggggat ggaatgcaat gccggtcacg gccagctccg
     7801 ccgcgatcgc gagcttgaac gccgcgagcc tgataacgga acgaaagcgc attaacacgg
     7861 cgaatcgcga atccaccttc gcatggctcg acagcaatgg ctacaagtac tcaccgtcgg
     7921 tgtcgaactg tttcatggtc aatgcccgca agccggcggg ggatgtgatc gccgccatgg
     7981 cgcaccagaa tgtgtacatc ggacggcctt ggtcgtcgat gcccgattgg gtccgcgtga
     8041 cggttggcac gcaagcggag atgaatcaat tccagacggc gtttcaggcg gtgatgaagg
     8101 gcgcgatcgt gggacgggtg tatgacgagc gcctccaccg gaatctcgac gggattatgg
     8161 tgacggggta ggcaagaagc aggttcaagt ttcgagtttc aagaaacgcg cgaaagcaga
     8221 actggctggg acggagggac accctgcggt ccctaacggg actcaaatca ttatttgtgt
     8281 cctgctcagc actccgcctg cggctgcgtg ctgggcaggc caatctcgcc ccaacgggct
     8341 aggccgtcgg acgctcttcc ctttgttccg aacttggagt tgtcttgccc gtgtattagc
     8401 gcgctgcagc tagcaggctg ctggtggtga gggctccgcc gccaacccga gccgggttat
     8461 gcagggtggc ttcgccggtg tcggggtggc gcagatcgac cagcagaatc ttgctgtcgg
     8521 aatagtaatc ctggccggag gcggttttgg ccttgaacac aggctcggcg ccctctacat
     8581 agctctcgtg cgcggtcacg gaggtctgcg acagtttcgc gccaatgaaa tcgcgctccg
     8641 aatcgacgtt ggggtcgatg cggtgattga ataacctgcc ccgttttgcg acttcaatgg
     8701 caacatcgtg agtcgctgcg cctgcccaga cggggttacc gtcgacttca tggccggtct
     8761 tccagatgcg aatgtgatga cggcgcgaga cgatggcatt gggatcgggc aaagcgtagg
     8821 cataatcctg tgtcctaccg aacaggaaaa acctggacat gggctgtctg acgtagtggg
     8881 tccgctgggt gactacgtgc caggcagact tcaacttcca gcgttcgggt ttgacccatc
     8941 cagcgtcttc aaaggctgac tgcacatctt ctttgtgacc gatgaacagg aggttcacca
     9001 tgtcaccctg gcggccgtcg ccgtccagca cgcgcggcgg caggaactgg gtgacctgat
     9061 tgagatcgga tgtgctggcg gtggtcaccg gaaagtcagc cgccgccaga gaagtagtgc
     9121 cgttgatttg tgctgtaatt tcggtgctct tgagggcagt gcaattgtcg ccgcgggtaa
     9181 gcaggaagac gggggcggcg ggcatataga agatgctggt aatcgccatc cccgcagcca
     9241 ttctccaggt gtgcgaggtc cccttcactt ccttgcgagc gcgcaagggt acgcgatcgc
     9301 cgttggccaa ctccaccgaa tcaagtttga ggacgaggtt gccacccatt cccagcacgc
     9361 ggcggccatg cacgcgagcc agtgagcctg acgcggtgct gccggcagga atgacggtga
     9421 aaccctgcac gtccacatcg cgcaccaccc ggaaactcag gcggtcccca
//
LOCUS       DASRKK010000722         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_368405, whole genome shotgun sequence.
ACCESSION   DASRKK010000722 DASRKK010000000
VERSION     DASRKK010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368405"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFC15_17510"
     CDS             complement(<1..207)
                     /locus_tag="VFC15_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12010.1"
                     /translation="MPFTLLDWTAIVGYLAITLIVGLWFRRRSSRSVDDYFVSGRNVN
                     WWLAGTSMVATTFAADTPLVVTGLV"
     gene            complement(272..490)
                     /locus_tag="VFC15_17515"
     CDS             complement(272..490)
                     /locus_tag="VFC15_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM12011.1"
                     /translation="MHHPWPTLLAVVDEVLPVNSTAVERARQIVLEYRRLSARDAIHL
                     SIMEQHGIERILSFDSRFDCFLGITQLV"
     gene            complement(510..>2659)
                     /locus_tag="VFC15_17520"
     CDS             complement(510..>2659)
                     /locus_tag="VFC15_17520"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HZM12012.1"
                     /translation="WASEFTKFPYRLFWWQAPDGSRLLTYFPSDYANPIDPQKMARDS
                     ATYGPMMWKYNGGTNSAPVGALDMMYLYGVGDHGGGPTRQDLDTALRWQKGDLVYPQI
                     NFSTAAQYFADLEKNKSELKIPTWDGELYFQYHRGVQTTQSEEKRGNRQNEELVLNAE
                     KVVSIETLFGASWPQQNFDTAWKDILFNQFHDILPGSGIAINYVDAARRYAAASRIDR
                     DMIHVGLNDIASRVKSDGVSVLVFNPLSWTRTDEIEADIQLPSETQHFYLQDSEGPVP
                     FEVLSSKQENNWMRLRMLARNVPATGYKLITVVPGGRGPHAQVVERPSPTGILASDTS
                     LENDFIRLKVDPKTGCITSLFDKRSNTEALALPVQSEGSPEGSPDGLPCGNLLQAFVD
                     KPKRWDAWNIDADFVKQHWDLMQADEVKLVENTPLRAVIRVRHHFQKSSFVQNITMVL
                     GVPRVDVHMQADWHEQHILLKVAFPVSAHSDKATFEIPYGSVERPTTRNTPAEQAQFE
                     VPALRWADISDATHGFSLLNDSKYGYDAKDNVLRLSLLRSPTWPDPETDQGHHEFTYS
                     IYPHGGTWREAMTVHQGYELNYPLMTQTTTQHPGSLPSEKSFFGTTEDNVVITAIKKQ
                     APQTPVQAVYDGQPRTVHPEEGALIIRFYEWAGKKGDIHLNLPQPGTAAWEMNLMEVA
                     QSPLSLAANPEVLTVPTSPYEIKTVKVQFFNHF"
BASE COUNT          498 a          802 c          797 g          562 t
ORIGIN      
        1 caccaggcca gtgaccacca gcggcgtgtc ggcggcaaag gtggtcgcca ccatcgaggt
       61 ccccgccagc caccagttca cgttgcgtcc ggagacgaag tagtcgtcaa cactgcggct
      121 ggaacgccgt cgaaaccaca gccccacgat gagcgtgatc gcgagatagc cgacgatggc
      181 ggtccagtcg aggagggtga agggcatgct tttcctgtct cctgagccgg tctctcaagt
      241 ggacactgta gtgcttcttt caggcgcccg cctacaccag ctgtgtaatg ccgaggaagc
      301 agtcaaacct cgaatcaaag ctcagaatcc gctcgatgcc atgctgttcc atgatcgaga
      361 gatgaatcgc gtcacgggcc gacagccgcc gatattccag aacgatctgc ctggcgcgct
      421 cgaccgccgt gctattgact ggcagaacct catcgaccac ggccaacagc gtcggccagg
      481 gatggtgcac ccggcgcgac ctccatacct tagaaatgat tgaagaactg taccttcacc
      541 gtcttgatct cgtacgggct ggttggaacg gtcagcactt cggggttcgc ggccagcgac
      601 aacggactct gcgcgacttc catcaggttc atctcccacg cggccgtccc cggctgcggc
      661 aagttgagat gaatatcgcc cttcttaccc gcccattcat agaaacggat gatgagcgca
      721 ccctcctcag gatgcacggt tcgcggctgg ccgtcataga cagcttgcac cggcgtttgc
      781 ggagcctgtt tcttgatggc ggtgatgaca acgttatctt cggtcgttcc gaagaacgac
      841 ttctccgacg gcaaggaacc gggatgttgc gtggtcgtct gggtcatcag cggataattt
      901 agctcgtagc cctgatggac cgtcatcgcc tcgcgccatg ttccaccatg cggatagatg
      961 gaatacgtga attcgtggtg cccctggtcg gtctcgggat cgggccatgt cggcgagcgt
     1021 agcagcgaga ggcgcagtac gttgtccttg gcgtcatatc cgtacttgct gtcgttcagc
     1081 agactgaaac catgggtcgc gtcggagata tcagcccagc gcagtgccgg aacctcgaac
     1141 tgtgcctgct cggccggcgt attacgcgtt gtgggccgct cgacgctgcc gtagggaatc
     1201 tcgaaggtgg ccttgtcgct gtgcgcgctg acgggaaacg cgaccttgag tagaatgtgc
     1261 tgctcgtgcc agtcggcctg catgtgaacg tcaacccgcg gcactccgag aaccatcgtg
     1321 atgttctgca cgaagctcga cttctggaaa tggtggcgca cgcgaatgac agcgcgcagc
     1381 ggcgtgttct ctaccagctt cacttcgtca gcctgcatca ggtcccagtg ctgcttgacg
     1441 aaatcagcgt cgatgttcca cgcgtcccaa cgcttgggct tgtccacgaa ggcctggagc
     1501 aggttgccgc acggcaaccc gtccggcgat ccctcgggcg agccctcgct ttgcacgggc
     1561 agagccagcg cttcggtgtt gctgcgcttg tcgaagaggc tggtgatgca gccggtcttg
     1621 ggatcgacct tcaagcggat aaagtcgttc tccaacgacg tgtcgctcgc caggattccc
     1681 gttgggcttg gacgttcgac aacttgagca tggggccctc gcccgccagg gacgactgtt
     1741 atcaacttgt acccggtagc tggcacattt ctcgcgagca tccgcagtct catccagttg
     1801 ttctcttgct tggagctcag aacttcaaac ggcacgggcc cctcggagtc ctgcaagtag
     1861 aagtgctgtg tctcagacgg aagctgtatg tcggcctcaa tttcatcggt tctcgtccag
     1921 gacagtgggt tgaagaccag cacgctcacg ccatccgact tcactcgcga agcgatgtcg
     1981 ttcagcccaa cgtgaatcat atcgcggtcg atgcgcgaag ccgcggcata gcgacgggcc
     2041 gcatcgacgt aattgatggc gatgcccgag cccggcagga tgtcgtggaa ctggttgaac
     2101 aggatgtcct tccatgcggt atcgaagttc tgctgcggcc agctcgcgcc gaacagggtc
     2161 tcgatggaga ctaccttctc cgcgttcagc acgagttctt cattctggcg gttgccgcgc
     2221 ttctcttccg actgcgtagt ctgcacgcct cggtgatact ggaaatacaa ctcgccatcc
     2281 caggtcggaa tcttcagctc gctcttgttc ttctccaggt cggcaaaata ttgcgccgcg
     2341 gtcgagaagt tgatctgcgg atagaccaag tcgcctttct gccagcgcag cgcggtgtcg
     2401 aggtcctggc gcgtcggtcc gccaccatga tcgccgacgc cgtacaggta catcatgtcg
     2461 agcgcaccca ctggcgccga gttggtgccc ccgttgtact tccacatcat cgggccgtag
     2521 gtggccgagt cgcgggccat cttctgcgga tcgatcgggt tggcgtagtc gctggggaag
     2581 taggtcagca ggcggctgcc gtctggcgcc tgccaccaga acaggcgata ggggaacttg
     2641 gtgaactccg acgcccaca
//
LOCUS       DASRKK010000723         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_465645, whole genome shotgun sequence.
ACCESSION   DASRKK010000723 DASRKK010000000
VERSION     DASRKK010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465645"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFC15_17525"
     CDS             complement(<1..324)
                     /locus_tag="VFC15_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12013.1"
                     /translation="MSVGPTVFVPGATVFTFGSTVLALGSAVFTLGVTVFALGVAGLA
                     LGVTGLALGIAGLALGTGLALGATVFALGAGLALGAARCAKHTPVSKPSSIPPTISFL
                     MPFCLR"
     gene            complement(1123..1527)
                     /locus_tag="VFC15_17530"
     CDS             complement(1123..1527)
                     /locus_tag="VFC15_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12014.1"
                     /translation="MTAILTLMFVRNPDEVPSPFLAPLGTAALRLLVEHRTEDIKALL
                     SREPVDAILLDQDHLQDSVVTELKRIAPRTPVILLRRRSQRAAIKPPGIAAVCCADPK
                     DEKVLNALPIFFGFILGKQVLHFRNRTLVPSA"
BASE COUNT          477 a          620 c          629 g          380 t
ORIGIN      
        1 tcgtaaacaa aatggcatga ggaaactgat tgttggtggt atgctgctgg gcttgctgac
       61 gggcgtgtgc tttgcacagc gcgcagctcc gagtgcgaga cccgctccta atgcgaacac
      121 ggtagctccc aacgccagcc cggtgcctag cgcgagcccg gcaattccca gcgcgagccc
      181 ggtgactccc agcgcgagcc cggcgactcc tagcgcgaac acggtgactc ccagcgtgaa
      241 caccgcagat cccaacgcca acacagtaga tccaaacgtg aacacggtag ctcctggaac
      301 gaacacggta ggtcctacgg acatgaccca gaagccgcca attcctccca atacggtagt
      361 cagtcctgac gcggcgcctc ccatcccgca atactaaaag gcacgaagta gcggctacat
      421 atggccgcag atcacagcga tacgcaaagc gatagaatcc gaaaggggta gctatccaga
      481 ctgccccttt tcggactcgg cgcaccgtgt gcggaacgtc attgcgatgc cagcatcaat
      541 tgacccgcgc ggagatgctc ttcccgagat cccgaggcag gcgggggcag gtgctcgtcc
      601 gatgcatcca caggccgatc aggtcggccg ggcactgcac tccgaatttg ctgaggaggt
      661 tggaggaact tcacggttcg ctcgcaaacg aagagtttgc gggcgatctc tttgttggac
      721 aggttgtcga gaagcaggcg agcactccgc gttcgcggcg gctgatgcgg cttcggtgtc
      781 gagtgacttg caggcctaca gttcgggaaa gaatagactc cacgcagcgg gtcaacagtt
      841 cgcgcggcag ccaatatcct ccgccggaaa cttggcgcgc cgcccggcac aactcgcgct
      901 gtgcctgcgg ataggtcagc acagaccttt cactccgagc tgcaacaggg gaaacgtcat
      961 ggaatcgtca aacagggcag cagcaccagc aggttcagcg tgcgctgttg ggtgcaaagc
     1021 atacgaacaa attcaagcgt agcgatcgca tcgctggcat ccacgaccgc caggcacgcg
     1081 tgaggagcgt gcgggccccg ctatacggct ggagtatatt ttctaggccg atggcacgag
     1141 cgtacggttg cggaaatgca gtacttgttt ccccaggatg aagccaaaga atatcggcaa
     1201 agcattcagc accttttcgt ctttcgggtc agcacaacag acggcggcaa taccaggagg
     1261 tttgatagcc gcgcgctgac ttcgacggcg caacaagatc accggcgtcc ggggagcgat
     1321 gcgcttgagc tcggtcacaa cgctatcttg caggtgatct tgatcaagga ggatggcatc
     1381 gacaggctcg cgcgacagaa gcgcctttat gtcttccgtg cgatgctcca ccagcaggcg
     1441 aagagctgcg gtccccaggg gagcgaggaa gggagaagga acctcatcgg ggttgcgcac
     1501 aaacatcagc gtcaagatcg cagtcatgcg accaaccttt cgaggccaga attattctca
     1561 ggtgcttatt gagacgcaaa gcagaaaact atgacagctg ccactcccgt aacctgggag
     1621 ctcagcagac gagggctgtt actaccttcc acaatccatg tccgttaagt caagaaaaaa
     1681 aatgcccacg cgacccattg ttaaagagaa gtttactttc ttagaaaccc aagctgtgcc
     1741 ccatcctcct agccgttcca tgaatgcaca ccggccgagg gcgagtgcgc cgtgcctccc
     1801 gagcttgggg cgcggcgcac gaaccgcgcg ttgtgggaat cgcggggcgt gcgaggcgtg
     1861 cgcggaatcg gcaataccaa tggccgaacg cccccgtgca gtcgggaaga atgggttcgg
     1921 cctcttgaga tggagccgct cagaacttgc cgatgcggat gctgccatcg cccgtggtga
     1981 tctccagcag cggtccgcca ccgtttaact tgccacgaat gcgtgaccgc tcgatggatc
     2041 cgttcaccgt caccggcatg tccagttgga tatggccgtc gccagtgtgg gcgtaaagct
     2101 ccgcgg
//
LOCUS       DASRKK010000724         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1444492, whole genome shotgun sequence.
ACCESSION   DASRKK010000724 DASRKK010000000
VERSION     DASRKK010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444492"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..625)
                     /locus_tag="VFC15_17535"
     CDS             complement(71..625)
                     /locus_tag="VFC15_17535"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HZM12015.1"
                     /translation="MARSNIKQTEHVIKVALEPTNSPEGTVAVASENVAAEANGTGGK
                     SLTIWEQVQRFGWQGWLLVAVFLGLYGQVLIRLVRQWNSDADYSHGFLVPFLSAYLIW
                     QRRDKLAQVVRRPSNWGLLVVIGSLGVLFLGSLGAELFLTRISIIGTICGLIVYFSGW
                     RLLRALAFPPGISVVCRANSDADL"
     gene            complement(1147..2505)
                     /locus_tag="VFC15_17540"
     CDS             complement(1147..2505)
                     /locus_tag="VFC15_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM12016.1"
                     /translation="MRRQLGSAAIPGWLLNSRGKLQQFEKYAWKVFAVPELLVTLQDK
                     RRAPAVPTFDVVNSLLHTALLRIPSLNALEGDLQESDFQRLIGRTPTPAVKAFSADVV
                     ANVLDKLHLGGVRRAIQQVIDKAERNKAFREGSYGALRCVAIDGWEPFASYNRHCPHC
                     LVRQVKVKRAHGETIEIEQYYHRYVVAMLLGPVVDVVLAIEPVLNEEARRDSDSQHPG
                     HEGELTAARRLLASLHQTYGGFIDAIVGDALYANGPWMTQLDRYGYGGFLVLKQENHE
                     PFKEALALWQGQPACETYDDPDSQEQIEFWEADDIDTLATYKGKLRVVRAVLAKPDKA
                     LTTWCFVILGQRARRVSRRTALKITRARWHIEDTAFHQWVRYWNFGHVFRHTANALLA
                     VLLLWTLAFNLLQLFVYRRLKRPRRPKDPTATIRHIVEVMLREVATLPAPIPWAALLD
                     TS"
     gene            complement(2702..3076)
                     /locus_tag="VFC15_17545"
     CDS             complement(2702..3076)
                     /locus_tag="VFC15_17545"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM12017.1"
                     /translation="MKMEWYDAKGDKAARQRQRKFALLRRFQIPPDALPGSLALTHIR
                     CGRPNCRCAQGERHPVWSLTFMVDGKKHVQHVPQELVEQIRPLVEQGREFKDAVAEVF
                     AANAQLLALWRQQSRRKPPKPR"
BASE COUNT          668 a          993 c         1078 g          645 t
ORIGIN      
        1 caggttcagc atttccaggc aactcgtggc aaaccgcgag gcaatgaact gcagcggaaa
       61 aacaatctcg ttatagatca gcgtcggaat tggcacggca aacaacagaa atgccagggg
      121 ggaacgccag ggcccgcagc aatcgccagc ccgagaagta aacgatcaag ccgcagattg
      181 tgccaatgat cgaaatgcgg gtaagaaaaa gttcggcgcc caaacttccc aggaacagca
      241 ctcccaggga accaatcacc accaggaggc cccagttgga gggacgccgc accacctgcg
      301 ccaatttgtc gcggcgttgc cagatcaggt acgccgacag aaacggaacc agaaatccgt
      361 gggagtagtc ggcatcgctg ttccattggc gcaccaggcg tatcaggacc tgcccataga
      421 gaccaaggaa gaccgcgacc agcagccagc cttgccatcc gaaccgctga acctgctccc
      481 agatggtgag cgacttaccg ccagtgccat ttgcttcggc agccacgttt tcggacgcga
      541 ctgccaccgt gccttctggc gaattcgtgg gttcgagcgc cactttaatc acgtgctccg
      601 tttgcttgat gttcgaccgt gccaaaaacg ccttgcatgt tgtgagccct cagcgtcacc
      661 cagcgtatcg catatccgcg tgaaaagtaa ccagttatta ccggggaagc tgcactgcgc
      721 aaaatccggg gtgaaccctc tcccagctct tccacttccg cactagctgg gttctcgttt
      781 gccaggcgtt gcccaccgca tccttagctc gtagctgaag tcgaaaggcg gcatcgatcg
      841 tgttaaagac tagttaacgg agcggctgat tcaccataca atcggcaggt tatagaatat
      901 caccacgcgc ctgcaaggaa cgccccggca ggccaggggt tccgcttctg cagttaagcg
      961 cacggctctt gaaccgctga gcaagccgcg cgagtggtag cgagcgcacg aggccgtcag
     1021 cttgtctcgc cggtgtgatt tgtcttgcga gtcgacttcc tccgaagcag gtaaagatgc
     1081 gggctaaggt taggagggcg ccggaccgcc gagccgccta cttgggcgcg ggctccgggc
     1141 gcggtattag ctggtatcca gcagggccgc ccagggaatg ggtgcgggca gggtggcgac
     1201 ttcgcgcagc atgacctcaa cgatgtggcg gatggtggcg gtgggatcct tgggccgacg
     1261 cgggcgcttc agccggcgat agacgaacag ctgcagcagg ttgaaggcca gggtccaaag
     1321 aagcaggacg gccaacaggg cattggcggt atggcgaaat acatgtccga agttccaata
     1381 ccggacccat tggtggaaag cggtatcctc gatgtgccag cgagctcgag tgatcttcag
     1441 tgccgtccgg cgggatacgc ggcgcgcccg ttggccgagg atgacaaagc accaagtcgt
     1501 cagggccttg tcgggcttgg caagcacggc ccggactacc cgcagctttc ccttataggt
     1561 agccagggta tcgatgtcat cggcctccca gaactcaatc tgctcttggc tatcgggatc
     1621 gtcataggtt tcgcacgctg gctgcccctg ccagagggcg agggcttcct taaagggttc
     1681 gtggttttcc tgtttgagca caaggaagcc gccatagccg tagcggtcca gttgagtcat
     1741 ccaggggccg ttggcataga gggcatcgcc cacgatggca tcgatgaacc cgccgtaggt
     1801 ctggtgcagc gaagctaaca gacgccgtgc ggcggtgagt tcgccttcgt ggccagggtg
     1861 ctggctgtcg ctatcgcggc gagcctcctc gttcagcacc ggttcgatgg ccaacacgac
     1921 atccaccacg ggaccgagaa gcatggcgac gacatagcgg tggtagtact gctctatctc
     1981 tatggtctcc ccatgggcgc gtttgacttt gacctggcgc accaagcagt gcgggcagtg
     2041 gcgattatag gaggcaaagg gctcccagcc gtcgatggcc acgcagcgca gggcgccgta
     2101 agaaccctcg cggaaggctt tgttgcgctc ggctttgtcg atgacctgtt ggatcgcacg
     2161 ccgcacgccg ccgagatgca acttgtccaa cacgttggcc acgacgtcgg cactgaaggc
     2221 tttcaccgca ggcgtgggtg tccgcccgat cagcctttgg aaatcgcttt cctgcagatc
     2281 accttccaga gcgttgaggc tggggatgcg cagcagggcg gtgtgcaaga ggctgttcac
     2341 gacgtcaaag gtgggaacgg ctggggcacg ccgcttgtcc tgcagagtga ctaatagctc
     2401 cggcacagca aagaccttcc aggcatactt ctcgaattgc tgcagtttgc cgcgggagtt
     2461 gagcagccac cccggtatgg cggcggaacc cagttgccgg cgcatgcctg ggtcgcgttc
     2521 ccaacagcag cgtttgtatt tattgccgct cccacagggg caggcatggt tgcgacccaa
     2581 tcgtggcgga agcgtaagcc cggcagcgct gctgcccgcg ctcggcaggg agtcggggaa
     2641 ggtcaagccc acagtcgcct gcaagtcgca gggcccgctc ggcggcttga ggttcgccgc
     2701 ttcaccgcgg cttgggcggc ttgcggcggg attgctggcg ccacagcgcc agcagttggg
     2761 cattggcggc gaagacctcg gccaccgcat ctttgaactc gcggccctgc tctaccaggg
     2821 ggcgaatctg ctccaccaac tcctgcggca catgctgcac gtgctttttg ccgtcgacca
     2881 tgaaggtgag cgaccagacg gggtgacgct cgccttgggc gcagcgacag ttgggtcttc
     2941 cgcagcggat gtgggtgagg gcgagagagc cgggcagcgc gtcgggaggg atctggaagc
     3001 ggcgcaggag ggcgaacttg cgctggcgct ggcgcgcggc cttatctcct ttggcgtcgt
     3061 accattccat cttcatgtaa tactacaata agaactggga tctgtcaacc ctcaccgttt
     3121 cgtcggggaa tttgcgcaag ccgatcccgg caagatgttc agcagatata cggggttaca
     3181 tgcctggagc ggaactcctg gcttagctcg gccacgccgg atcgcaggca attaccgcag
     3241 gccgcacagc attgctgatg tcactgtggg ctgggccggc tgctggccgt gagcctggtc
     3301 aaagcgtttc ttacctgcgt ggcttcggcg ccggtcggag tctcatgaag gtaggtttcg
     3361 tactccgtgt acgcgccctg gggc
//
LOCUS       DASRKK010000725         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1052917, whole genome shotgun sequence.
ACCESSION   DASRKK010000725 DASRKK010000000
VERSION     DASRKK010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052917"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFC15_17550"
     CDS             complement(<1..180)
                     /locus_tag="VFC15_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12018.1"
                     /translation="MKNLIAVSLVYGGLLVMLVGAVSLLWPLRPLAIRSRATGAIVLF
                     CGVLIFVIGAILPAGE"
     gene            complement(299..796)
                     /locus_tag="VFC15_17555"
     CDS             complement(299..796)
                     /locus_tag="VFC15_17555"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZM12019.1"
                     /translation="MPEKETLERAREDAREGKAPSTQAGEFVKEEMDHIREGKHGAAS
                     TKQAIAIGLSKARRAGVKLPPPGKGKTSEATRKKAAGDLRKGQSSAKKKTSAKRSRAI
                     KRVLKREGHQAASHAALSKQARQSARTRGSAARKASARKASQTKGSAGRKKAARKAAR
                     TRKRS"
     gene            847..>2724
                     /locus_tag="VFC15_17560"
     CDS             847..>2724
                     /locus_tag="VFC15_17560"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM12020.1"
                     /translation="MDYTNPARSRADMPAAVAQSVLAPPAMRSHKYRPALLAAALLLA
                     ATAIVYSVAWMYYVRGEPTVEIGIDTNYLDQAIEVTNVYHDSPAEKAGLKPHDRITAI
                     NGVPIPPRKTGFDVLQSIWVKSRPGDKVILRIERPGEANPLTLTATFRAIKGQGDTKT
                     LGRTVAEQILDSYPVLFLIVGLTVLFLRVDNRDAWLLAMVFASFITAPGLPRSFGVAP
                     DGLRHFLLAFNTIVGGLLPALFYFFFAVFPTRSPIDRKAPWLKWLLPGIGICLGYGGV
                     RYGDLAMLPFLQAVLSQRTGHNMRIVYAYGTILLGILSLILNLLGTSNPEARRKLKVI
                     VWGTAVGVTPVAVVKAAQDTVHFQTPFWLGFFCVMLLFLFPLSFAYAVVKHRVMDVPV
                     LLKRSARYFLVERGFVFLILAISVGATFWLAQVFSRLFSAGSKAAIPVGATFGVLLIS
                     GATQVHRRVRTRLDRAFFRSSYDAQQILESLAAKTLTVSSREGLAALLHDQIQDALHP
                     SSMYVYLEAGSGQLQAYAGDPPPEAMTLSTSAAGIAELAERSGPLELLPEAMHGTQLQ
                     PLQPECLVPIRGSSEGQLQGVAVLGPRLSEEPYSTSDKRLLASVASQAGIAMRSITLA
                     EK"
BASE COUNT          480 a          871 c          787 g          586 t
ORIGIN      
        1 ttcgcccgcg ggcagaatgg cgccgattac gaaaattaga actccacaga agagaacaat
       61 ggcgccggtg gcacggcttc gaattgccag cggtcgcaag ggccagagca gagacactgc
      121 tcccaccagc attaccagca ggccgccgta aaccagcgaa acagcgatca ggtttttcat
      181 ggactgagat ttccggtcag ctaaccattc tgcgctcagg ctgccttggc gaacagcgaa
      241 aagcgctcat acctagcccg agactcccag ggaaaaaact tgtcgcaaaa acagaaattc
      301 agctccgttt cctcgtccgc gctgctttgc gcgccgcctt cttgcgccct gccgagcctt
      361 ttgtctgact ggccttcctc gccgacgcct tgcgcgcagc gcttccccgt gtccgggccg
      421 actgccgggc ctgcttcgac agcgccgcgt gcgacgccgc ctgatgacct tcgcgtttca
      481 acacgcgttt gatcgcccgc gaacgtttgg cggatgtttt cttcttcgcc gatgactggc
      541 ctttacgcaa atcgcctgcg gccttttttc gcgtcgcctc cgaggtcttc cccttcccgg
      601 gcgggggcag cttcacgccg gcacggcgcg ccttcgacaa accaatggca atcgcctgtt
      661 tggtcgaggc cgcaccgtgc ttgccttcgc ggatgtggtc catctcctct ttcacgaatt
      721 cgccggcctg ggtgctcggc gcttttccct cgcgcgcatc ttcgcgggcc cgctccaacg
      781 tttctttctc tggcatcgat tctccttctc gtaccctcaa cagagatgca gaatccggcc
      841 tgcgggatgg actacactaa ccctgcgcgc agccgcgctg acatgcctgc agccgtagct
      901 caatccgtcc tggcaccccc cgccatgcga tcgcacaaat accggccggc gcttcttgcc
      961 gcagcactcc tgctggcggc gaccgccatt gtctacagcg ttgcctggat gtactacgtt
     1021 cgcggcgaac ctacggtaga gatcggcatt gatacaaact accttgatca agcgattgag
     1081 gtaaccaatg tttaccatga cagcccggcg gagaaggccg ggctgaagcc gcacgaccgg
     1141 attaccgcca tcaacggcgt acccatcccc cccaggaaga ccgggttcga cgttctgcag
     1201 tccatctggg taaagtcacg gcccggcgac aaggtgattc tgcgaatcga acgacccgga
     1261 gaggcaaacc cgctgactct gacggccacc tttcgcgcca taaaagggca gggtgacacc
     1321 aagaccctgg ggcggaccgt agcggaacag attctggact cctatccagt gctgtttctc
     1381 atcgtgggac ttaccgtact gtttctgcgc gtggacaatc gcgatgcctg gctgctggcc
     1441 atggtgtttg ccagcttcat caccgcgccc ggccttcccc gttcctttgg ggtagcgccg
     1501 gacgggctcc ggcatttcct gctcgcgttc aacaccattg ttggtggcct gcttccggcc
     1561 ttgttctatt tcttcttcgc ggtatttccc acgcgatctc ccatcgaccg caaagcgccg
     1621 tggctgaagt ggctgcttcc aggaattggc atctgcctgg gatacggagg ggttcggtat
     1681 ggcgatcttg cgatgctacc cttcttacag gcggtattaa gccagcggac gggccacaac
     1741 atgaggattg tgtatgccta cggaaccatt ctcctgggta tactttcgct catcttaaac
     1801 ctgctgggca cttccaaccc ggaggcccgg cgcaagctca aggtgattgt ctggggcact
     1861 gcggtcgggg tcacgcccgt ggctgtggtc aaagccgcgc aagacacggt gcacttccag
     1921 acgcctttct ggctgggctt cttctgtgtc atgttgctgt tcctgtttcc gctgtccttc
     1981 gcgtacgcgg tggtgaagca ccgggtgatg gatgtgcccg ttctgctgaa gcgcagcgcc
     2041 agatatttcc tcgtggagcg cggattcgta ttcctcatcc tggccatttc ggtgggggcg
     2101 accttctggt tggcgcaagt attctcccgc cttttctccg ccggatcgaa ggccgccatt
     2161 ccggtgggcg cgacctttgg tgtgctgctc atctcgggcg caacccaggt gcatcggcgg
     2221 gtgcgcacgc gactggaccg cgccttcttc cgcagctcct acgacgcgca acagattctg
     2281 gagagcctgg ccgcgaagac actgactgtc agcagccgcg aaggactggc ggcgctgttg
     2341 catgaccaga ttcaagatgc gctgcatccc agctcaatgt atgtgtatct ggaagccggc
     2401 agcggacaat tgcaggcgta cgctggggac cctccgccgg aagcgatgac actctccacc
     2461 agcgccgccg ggattgccga actggcggag cgcagcggtc cgctggaact actccctgaa
     2521 gccatgcatg gaacgcaatt gcagccgctg caaccggagt gcctagtgcc gattcgcggc
     2581 tcgagcgaag gccagttaca gggcgttgcc gtgctggggc caagattgtc ggaggaaccg
     2641 tactccacca gcgacaagcg tctgctggct tcggtggcca gccaggccgg tattgccatg
     2701 cgtagcatta ccctggcgga gaag
//
LOCUS       DASRKK010000726         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1444865, whole genome shotgun sequence.
ACCESSION   DASRKK010000726 DASRKK010000000
VERSION     DASRKK010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444865"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFC15_17565"
     CDS             <1..829
                     /locus_tag="VFC15_17565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM12021.1"
                     /translation="LIDNEILMQRAEKLGLLATDDEVNSKLAEIKAPFSQQDFDKRLA
                     DRKITLADFKADLRRSITVEKVLNKEVTSKINVTDEDITAYYEQHKAEFNLIEPQFHL
                     AQVLVTSQPNPQTRNVANKAQNDAEARKKIQMIENRLDSGEDFASVAMNFSEQPETSQ
                     NGGDLGFIPESSLKSDKIAYESIGKLKPGQYTTVMVVADPNSHQVFGYRVVRLISKES
                     AGQRELKDPRVQQAIREQLRDRREQLLKAAYYEVIRDEAKVENFFADDILKKTGATK"
     gene            complement(872..2038)
                     /locus_tag="VFC15_17570"
     CDS             complement(872..2038)
                     /locus_tag="VFC15_17570"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF005456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HZM12022.1"
                     /translation="MKKDWTTKLIHSDVRVPEGFRSLATPVYRGSTTLFSDAADVDDN
                     WDQYEVGYTYGLYGTPTTLELAGRICEIEGGYRTLITPGGQCAISTINFAFLKAGDHI
                     LVPESVYGPNRKLARSVLKRFGVETTFYPPTIGREIEALFQPTTRLLWVESPGSITME
                     VQDVPAIAEVAHAHGALVVMDNTWAAGVLFDALAHGVNITMQAVTKYIGGHSDLLLGS
                     ITVKDEARWKTLGAHQQVLGGAASPDDCSLALRGMKTMAVRLKAIERSALIIAEWLSR
                     RAEVELVLHPALPSCPGHEFWKRDFLGSSGLFSVVFKPGVTKQQVLAFVNALELFKIG
                     YSWGGVASLAVAYDFTGFRGRPDYGHRIVRLHTGLEDKDDLIADLEQGLTKLSQ"
BASE COUNT          467 a          668 c          589 g          401 t
ORIGIN      
        1 attgatcgac aacgaaatcc tgatgcagcg tgctgaaaag ctcggcttgc tggccaccga
       61 cgatgaagtc aacagcaagc tggctgagat caaggccccg ttcagccagc aggatttcga
      121 caagcgcctc gccgaccgca agattacgct cgccgatttc aaggcggatt tgcggcgctc
      181 catcaccgtg gaaaaggtcc tcaacaagga agtcacgtca aagatcaacg ttaccgatga
      241 ggacattacc gcctactacg agcagcacaa ggccgagttc aacctcatcg agccgcagtt
      301 ccacttggcg caagtcttgg tgaccagcca gcctaatccg cagacccgca acgtcgccaa
      361 caaggcccag aacgacgcgg aagctcggaa gaaaatccag atgatcgaga accggctcga
      421 cagcggagag gatttcgctt ccgtcgccat gaatttttcc gagcagcctg aaacctcgca
      481 gaatggcggc gaccttggct tcatccccga gtcctctctc aagagcgaca agatcgctta
      541 cgagtcgatc ggcaaactga aaccgggaca gtacactacc gtgatggtgg tggctgaccc
      601 gaacagtcat caggtcttcg gctatcgcgt ggttcgcctc atcagcaagg aatccgcggg
      661 acaacgcgag ctgaaggatc cgcgcgtgca gcaggcgatt cgcgagcagc ttcgcgaccg
      721 tcgcgagcaa cttctaaaag ccgcctatta cgaagtcatc cgcgatgagg ccaaggtgga
      781 gaacttcttc gccgatgaca tcctgaagaa gactggcgcg acaaaataat ctcgcctgcg
      841 cctcatggtg cgctccgcag ggcgagtgag atcactggct cagcttcgtc agcccctgtt
      901 ccaggtcggc gatcaaatca tccttgtcct ccaacccagt gtgcaaccgc acgatgcggt
      961 ggccgtagtc aggacgccct ctgaatccgg taaagtcgta ggcgaccgcc aggctggcca
     1021 cgcctcccca gctataacca atcttgaaca attcaagagc gttcacgaac gccagtacct
     1081 gctgcttggt tacgcccggc ttgaacacga ccgagaacaa tcccgaggat ccgaggaagt
     1141 cgcgcttcca gaactcatgt cccggacacg acggcaacgc aggatgtaga accagctcga
     1201 cctcagcccg ccggctgagc cactccgcaa tgatcagcgc cgaacgttcg atcgctttca
     1261 gccgcaccgc catggtcttc atgccgcgca gcgcgagact gcagtcgtct ggagacgccg
     1321 cgccgcccag cacctgctga tgcgcgccca gcgtcttcca tctggcttcg tctttcaccg
     1381 tgattgagcc cagcagcaga tcgctgtggc caccgatata cttcgtcacc gcctgcatgg
     1441 tgatgttaac gccatgcgct agcgcgtcga acagcacgcc cgcagcccac gtgttgtcca
     1501 tgaccaccaa cgcgccatgg gcatgcgcca cttcggcaat cgcgggaacg tcctgcacct
     1561 ccatggtgat cgaccccgga ctctcaaccc acagcagccg cgtcgtcggc tgaaacagcg
     1621 cctcgatctc tcgcccgatg gtcgggggat agaacgtagt ctcgacgccg aagcgcttca
     1681 gcacggatcg cgccagcttg cggttcggac cgtacacgct ttccggcaca agaatatggt
     1741 cgccggcttt caggaacgcg aagttgatgg tcgagatggc gcactggcct cccggtgtga
     1801 tcagggttcg gtagccgcct tcgatctcgc agatacgccc cgccagctca agtgtcgtag
     1861 gcgttccgta taggccgtag gtgtatccca cttcgtattg gtcccagttg tcgtcaacgt
     1921 cggcagcgtc agagaacagc gtggtggagc cgcgatacac cggtgttgcc agtgagcgga
     1981 agccctcggg cacgcgcaca tccgagtgga taagcttggt cgtccagtcc ttcttcatgc
     2041 gagcattata ggagggtgat gtgaacggcc ggggacattc ctgacatatt agaccgggca
     2101 catgcctgac tctttctcct gtttt
//
LOCUS       DASRKK010000727         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_172607, whole genome shotgun sequence.
ACCESSION   DASRKK010000727 DASRKK010000000
VERSION     DASRKK010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172607"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="gltX"
                     /locus_tag="VFC15_17575"
     CDS             <1..1144
                     /gene="gltX"
                     /locus_tag="VFC15_17575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZM12023.1"
                     /translation="ALQHQQPPIYSGKCRALDPEESQRRHANGEAAAIRLRIPEHPIR
                     FHDIVHGPVEFSNEVVSDPIILRSTGIPVYNYVVVVDDALMDITHVIRGDDHLSNSPK
                     QVALYEALGWPVPEFAHLSTILGSDRERLSKRHGATSIANFRQMGVLPEALVNYLALL
                     GWAPTGGTREIFSPEELIKEFSLQRVTPSPAVFDMEKLYWLNRHYIKQSPPERIEKLA
                     EPFFIRAGMLPENLDDRTRAWFGKVVALLGPSVDKGEELPERAGLIFRYDAAAAVNSP
                     DNLEVLAAAKTPDVLTAFAAQVEGDSSPMTPERFKAIMNDVKAKTGAKGKDLFHPVRV
                     ALTGSHSGPEFDKLIPILEQGSQLDLPQHVMNVGERLAAFRAARGM"
     gene            complement(1168..2223)
                     /gene="add"
                     /locus_tag="VFC15_17580"
     CDS             complement(1168..2223)
                     /gene="add"
                     /locus_tag="VFC15_17580"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZM12024.1"
                     /translation="MKSPLLPDPSNDFILKLPKAELHLHLEGTVDPPTLAELSRRHPT
                     PLPSSNNRYKNIEDSGRVFSEEEARALYEYKDFTGFLVAFKAVTERLRTAEDYELVTY
                     RMMQKLHAQNVLHAEVYISAGVVQWRGQEFAPLFEGAERGRQRGEREFGMSLLWIFDA
                     VRHFGVDEARRVVEDAIRFKGRNVAGIGIGGDERRAGPEQFREVYEHAAQHGLRLTVH
                     AGETVGAESIWGALRELKADRIGHGLHAVDDPELVRYLAEKQIPVEVCITSNVMTGCC
                     TGVEQHPVRKLFDAGVRVTLNTDDPDMFHTDLLREYQIAQQVFGFSEAELRELARNSF
                     RASFLPEEKKREYLARF"
BASE COUNT          504 a          761 c          698 g          512 t
ORIGIN      
        1 tgctctgcaa catcagcagc cgccgatcta ctccggaaaa tgccgcgcgc tcgatcccga
       61 agaaagccag cgccgccacg ccaacggcga agcggcggcg atccggctgc gcattcccga
      121 gcaccccatc cgctttcacg atatcgtgca cggcccagtc gagttctcca acgaagtggt
      181 cagcgatccc atcatcctgc gctccaccgg tattccggtt tacaactatg tggtggttgt
      241 ggacgatgcg ttgatggaca tcacgcacgt gattcgcggc gacgatcatc tctccaattc
      301 gcccaagcag gtggcgctct acgaggcgct gggatggccg gtgccggagt tcgcacatct
      361 gtccacgatt ctgggcagtg accgcgaacg cctgtccaag cggcacgggg caacctccat
      421 cgccaatttc cgccagatgg gcgtgctgcc cgaggcgctg gtaaactacc tcgcgctgct
      481 gggttgggcg cccaccggtg gcacacgcga aatcttttcg cccgaagagt tgatcaagga
      541 attttcgcta cagcgtgtca ccccgtcgcc ggcggtcttc gacatggaga agctgtactg
      601 gctcaaccgc cattacatca agcaaagtcc gcctgagcgc atcgagaagc tcgccgaacc
      661 gttcttcata cgcgcaggaa tgttgccaga gaatcttgac gatcgcaccc gtgcctggtt
      721 cggcaaggtt gtcgctctgc ttgggccctc ggtggataag ggggaggagc ttcccgagcg
      781 cgccggattg atcttccgct acgacgcagc cgctgcggtg aattcgcccg ataacctgga
      841 ggtcctcgcc gcagcgaaaa ctcccgacgt cctgaccgca tttgcggcgc aggtggaagg
      901 cgattcgtcg ccgatgacgc cggagcgctt caaagccatc atgaacgacg ttaaggcgaa
      961 gactggcgcg aagggcaagg acctgttcca tcccgtgcgg gttgcgttga ccggatcaca
     1021 ctcgggcccc gagttcgaca agctcatccc gatcttggag caaggatcgc aactcgatct
     1081 tccgcagcac gtaatgaatg ttggcgaacg cctggccgcg ttccgtgcgg cacgcgggat
     1141 gtaagaagta accggtgaac aaggttttca gaacctcgcc aggtattctc gcttcttttc
     1201 ctccggcaag aacgacgcgc gaaacgaatt tcttgccagc tcacgaagtt cagcctcgct
     1261 gaagccgaac acctgctgcg caatctgata ttcacgcaac aggtccgtgt ggaacatgtc
     1321 gggatcgtcc gtattcagcg tgacccgcac accggcatcg aacaacttcc gcaccggatg
     1381 ttgctccact ccggtgcagc agccggtcat cacattgctg gtgatgcaca cttccaccgg
     1441 aatttgtttc tcggcgagat aacgcaccag ctcgggatcg tcgaccgcgt gcagtccatg
     1501 cccgatgcgg tccgccttca attcccgtag cgcaccccag atggactccg ctccaaccgt
     1561 ttccccggca tgtacggtca accgcaaccc gtgttgcgct gcatgctcgt atacttcgcg
     1621 gaactgctcc ggcccggcgc ggcgctcgtc tccgccaatt ccgatgcccg caacgtttcg
     1681 ccccttaaag cgaattgcat cttcaaccac gcgacgcgct tcgtcgacgc cgaaatgccg
     1741 gaccgcgtcg aagatccaga gcagtgacat gccaaactcg cgctcgccgc gctgtcgccc
     1801 acgctccgcc ccttcaaaca gaggggcaaa ttcctgtccg cgccactgca ccacgcccgc
     1861 cgagatgtag acctcggcat gaagaacgtt ctgcgcgtgc agcttctgca tcatgcgata
     1921 ggtgacaagc tcgtagtcct cggcggtgcg caaccgttcg gtgaccgcct tgaacgcgac
     1981 caggaagccg gtgaagtctt tgtattcgta caacgcacgc gcttcttcct cgctgaagac
     2041 gcgtccgctg tcctcgatgt tcttatagcg attattgctg ctgggaagcg gcgtgggatg
     2101 gcggcggctc agctccgcca gggtcggagg atcgaccgtg ccttcgagat gcagatgcag
     2161 ctcggccttg ggcaatttta ggatgaagtc gttagagggg tcgggaagaa ggggcgattt
     2221 catgcaagtt gaacagacct aaagtaccgt ggggctgaca gccctgaata tcagtgcgcc
     2281 ctgaacgcga cgcgcttaac gtgcattatt catgatgatc tgacgctttg aagaggcacg
     2341 gcttcagagg ctgctgaaaa atgaaccaaa gtagtaattt cgagcgtttc ttgactcaaa
     2401 agtcactcgt aagttattga atcgtcgttg cgcaaaatgc cgagtttact tgtttgagtc
     2461 ttttttcagc agcct
//
LOCUS       DASRKK010000728         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_269626, whole genome shotgun sequence.
ACCESSION   DASRKK010000728 DASRKK010000000
VERSION     DASRKK010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269626"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFC15_17585"
     CDS             <1..378
                     /locus_tag="VFC15_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12025.1"
                     /translation="IVGGGDLTTNTAAQAATSEDGGQTWALTNQPPIPGAIFCLAYAR
                     GLAHDDDSVNQDHFGHEHDRTVVITTETQPNFDSGAAAWTPDEGQTWFTLPQVSGYWA
                     VAFANPEAGWFVGNNGQILKISF"
     gene            complement(897..2366)
                     /locus_tag="VFC15_17590"
     CDS             complement(897..2366)
                     /locus_tag="VFC15_17590"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HZM12026.1"
                     /translation="MTVRGRNFVALVGVLALMAGSVAAQQDSVAGKAPKQGTQVPPAQ
                     AKALEDWSYSLAVQAATWGSPAVIMYALRDHDATGPNSKAPPNGLWRMENTSTPELAE
                     KAGYVLPNLSVIYGFGFLDLRQEPVVLSLPDSNGLYYMVETVDMWTNAFAYPAGAEAG
                     YKGGKVAYVAPGWQGELPPGVKRIASPTPWVLIQPRVHLPQQSGLEAARKVLAEIKPQ
                     TLSEYLGKAAQPAPKNNYAVPNLINPKLPVSTLDFKDPLQFWEIMSAVINENPPPKDE
                     IAAVLPMFAPLGIELGKQWDRTKVNPIVLKAMSRAAVDVPKILATLPIGQPVNGWFIP
                     SATMGVYGTDYSLRAYVARNGLTANTPEEAVYLQAVLDGADQPLNGTKQYTMTFKQPP
                     PFNEPAFWALRMFDATNYYPVPNPINRYVLGSDYPDMKKNADGSLTIYLQSKNPGKDK
                     EANWLPTPTGPFMLILGTYAPGKALIDGTYVPSPAVIVK"
     gene            complement(2665..3441)
                     /locus_tag="VFC15_17595"
     CDS             complement(2665..3441)
                     /locus_tag="VFC15_17595"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZM12027.1"
                     /translation="MNGIPHVSPAHLTSSRAMVIKPILAEEISKNPCCSGNREGLRSA
                     LGVGGDYYDFLALPAGKLGIAIGDVSGKGIAAALTMASLQASLRSEATRAPDNLAGMM
                     SNVNRLVYEASASNRYATFFYGQYDPASRQLTYVNAGHNPPMLFHCSDGEWPVCRLET
                     GGTVVGLLESFSYEQATLTIAPGDILIAFTDGISEAMNDAQDEFGEDRLIETIKRCAV
                     LSPAEIIACIMRTADAFVAGAKQNDDMTLVVLCAQTGTGK"
BASE COUNT          689 a         1034 c          999 g          754 t
ORIGIN      
        1 attgtgggtg gcggcgactt gaccacgaac accgctgccc aggccgcaac ctcggaggat
       61 ggcggccaaa catgggcgct taccaaccag cctcccattc caggagcgat cttctgtctc
      121 gcctatgcgc gtggccttgc acacgacgat gattcggtca atcaagacca cttcggccat
      181 gaacacgacc ggaccgtcgt gatcacgacc gagactcagc cgaacttcga ctccggcgca
      241 gccgcttgga ctcccgatga aggacaaacc tggtttacac tgccgcaggt gagtggttac
      301 tgggcggtcg cttttgcgaa tccggaagcc ggatggttcg tcggcaacaa cggccagatt
      361 ctgaagatca gcttctagtg tcccgagtct ctttcacacg agacaagcgt gcgtagcctg
      421 cttgactgta tggtgtacgt cctccttgtg gggagcttct atctgagtgc gacagcaaat
      481 gccaagacca atcttttcgt tcgggtctgc atgacagccc attatgccac ctccgggctg
      541 atggcgaaac ccatatggac gaaaaacgcc ctacgcgcgg acgtggcgca ccgaaagcgc
      601 gggtccgtca cacttccacg cgtagccgac tacacggcca agtgaaactt cctccaacaa
      661 gacttcgggc ccggaaccaa ttcccgtttg ctcgtgactc gcgccggacc gttgggaagc
      721 gggcgctaga cgtctttcgt tgtgagtttg cgatttcgta atagctatcc gagagtggtg
      781 aggagtcagt ttccgaatca tcgacaatgc accaactgtg tggtgccgaa aggaagaggg
      841 cgtgcacttg tgcacgcccc ttgttgtcgc cgcaattcta gccggccgca ggaactttat
      901 ttgacgatca cggccggcga cggaacgtat gtgccatcga ttagtgcctt acccggagcg
      961 taggtgccaa ggatcaacat gaacggtcct gtgggtgtgg gcagccaatt cgcttccttg
     1021 tccttcccgg gattcttgct ctgcaagtag atggtcaacg atccatccgc gttttttttc
     1081 atgtctgggt agtcactccc caggacataa cgattgatcg gattcgggac ggggtagtag
     1141 ttcgtggcgt cgaacatccg caacgcccag aaagccggtt cgttgaaggg cggcggttgt
     1201 ttgaacgtca tggtgtactg cttggttccg ttgagcggtt ggtccgcgcc atccaggact
     1261 gcttgcaaat agaccgcttc ttcgggcgtg ttggcggtca agccattccg cgcgacatag
     1321 gcgcgaagac tgtaatccgt gccatagacg cccatcgtgg ccgacgggat gaaccagccg
     1381 ttgacgggct gacctatcgg cagcgtggcc agaatctttg gcacgtccac agccgctcgg
     1441 gacatcgcct tcaggacaat cgggttgacc ttggtccggt cccactgctt gcccagttcg
     1501 atgccaaggg gcgcgaacat cggcaggacg gcggcgatct cgtccttggg aggagggttc
     1561 tcgttgatga ccgcggacat gatctcccag aactgcagcg ggtccttgaa atccaaggtg
     1621 ctcaccggca atttcggatt gatcaagttc ggcacggcat agttgttctt gggagccggt
     1681 tgtgccgcct tgcccaagta ttccgacagt gtctgcggct tgatttctgc caagactttt
     1741 cgggcggctt ccagaccgct ttgttgcggt agatgcaccc gaggctggat caaaacccaa
     1801 ggcgtcggcg aagcaatgcg cttgacaccc ggcggcaact caccctgcca gcccggagcg
     1861 acataggcga cttttcctcc cttgtaaccg gcctccgcgc ccgccggata cgcgaaggcg
     1921 ttggtccaca tgtcgacagt ttccaccatg tagtagagcc catttgaatc gggaaggctc
     1981 aggacgaccg gctcctgacg caggtcgaga aaaccgaagc cgtagatcac gctcaggttc
     2041 ggcagcacat aacccgcttt ttcagcgagc tccggagttg aggtgttctc catccgccag
     2101 agaccgttcg gcggcgcctt ggaattgggt cccgtggcat cgtgatcgcg cagggcgtac
     2161 atgattacgg ctgggctacc ccaagtagca gcctggacgg caagcgaata cgaccagtct
     2221 tccaaagcct tggcctgcgc tggcgggact tgtgtcccct gcttcggggc cttcccagcg
     2281 accgaatcct gctgggccgc gaccgacccg gccatcagcg ccagcactcc aaccaaagca
     2341 acgaaattcc tacctcggac agtcattgat ccttccattt ctcaggtctt tgttgggtga
     2401 cccgctattc gttcaggaag gatcatggtc gcagtccgca gcacagccat gctgtgattt
     2461 gagtcaccat gctgtgtaat tcagactagg gggcgagcgc cgggcgactc cgaagtcagc
     2521 tttccagaga tagctgtagg catcatctcc tcgaaccaga cttctggccc acaataacgt
     2581 ccggtttgcc cgtcactcgg gcgtaacgag cgttcgcctt ctgcctggcg cccgagtccc
     2641 gtttcctgct tgaagcgtag ccgcctattt gccagttccc gtttgggcac agagcacgac
     2701 cagcgtcatg tcatcgttct gcttggcccc ggcgacgaac gcgtcagcgg ttcgcataat
     2761 gcaggcaatg atctcagccg ggcttaagac tgcgcagcgt ttgatggttt cgatcagccg
     2821 gtcctcgcca aattcatctt gtgcgtcatt catggcctcg ctaatgccgt ccgtaaaagc
     2881 gatgaggatg tctcccggcg cgatcgtcag agtcgcttgc tcataggaga agctctccag
     2941 caaacccacg actgttccgc ccgtctccaa ccggcacacc ggccattccc cgtcggaaca
     3001 atggaacaac atgggcggat tatggccggc gttcacataa gtgagctggc gtgaagcagg
     3061 atcgtattgc ccgtagaaga aagtcgcata gcgattggac gccgacgctt catagaccag
     3121 ccggttaacg ttgctcatca tccccgccag gttgtcgggc gcccgtgtgg cttcgctgcg
     3181 aagagaggct tgtaagctgg ccatcgtcag cgctgcggca atgcctttgc cggagacatc
     3241 gccaatcgcg atccctagtt tccctgcggg caaggccagg aaatcgtagt aatcgccgcc
     3301 cacaccgagc gccgaccgaa gaccttcgcg attgccggaa cagcatggat tcttgctgat
     3361 ctcctcggcg agtatcggct taatgaccat cgcccgcgat gacgtgaggt gtgccgggct
     3421 cacgtgcggt attccgttca agcctatatc cgccggattg atcgctcatt gacgcg
//
LOCUS       DASRKK010000729         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_954922, whole genome shotgun sequence.
ACCESSION   DASRKK010000729 DASRKK010000000
VERSION     DASRKK010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954922"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFC15_17600"
     CDS             <1..286
                     /locus_tag="VFC15_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12028.1"
                     /translation="GRGVAMKHLISSMRIAFRALKMNKLRSALTMLGIVIFGFGASLL
                     ISRLADWATSVGPGAVLLAVLFSALVGIGFGYYPARTAAYLDPIEALRNE"
     gene            complement(367..2805)
                     /locus_tag="VFC15_17605"
     CDS             complement(367..2805)
                     /locus_tag="VFC15_17605"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZM12029.1"
                     /translation="MNTITAPSRQNNTSHIGIAPLTPDELRRMDAYWRACNYLCAGML
                     YLRDNPLLREPLKPEHIKNRLLGHWGSDPGQTFTWVHLNRLIKKYDLDLIYVSGPGHG
                     APATLANSYLEGVYSEIYPEMGEDAVGMQKFFKQFSFPGGIGSHCTPETPGSIHEGGE
                     LGYSLSHGFGAAFDNPDLIVTVVVGDGEAETGPLATSWHSNKFLNPICDGAVLPVLHL
                     NGYKIANPTILARIPSDELESLFVGYGWTPYVVEGSDPALMHQEMASVMEHCVLEIRA
                     IQEKARSVGVGVAPERPRWPMIILRTPKGWTGPKELDGHKVEGSWRAHQIPILDPVTN
                     PAHLKLVESWMRSYRPEELFGANGTLIAELKDTAPRGARRISGNPHTNGGTLRKPLDL
                     PNFREYAVNVAQPGQTEVSSTDTLAHFLRDVMRRNMKSFRVFGPDETASNKLQVIYEA
                     TGKTWMAEMKPEDADGGDLATDGRVMEMLSEHTLEGWFEGYVLTGRHGFFSSYEAFVH
                     IIDSMFNQHAKWLEKSKLELRWREPISSINLLITSLVWRQDHNGFTHQDPGFLDLVTN
                     KSPEVTRIYLPPDANCLLSVTDHCLRSTNYVNVIVADKALHLQYLEMDEAVKHCTKGI
                     GIWDFASNDAGQEPDVVMACAGDIPTSESLAAVAILRERCPDLKIRFINVVDLFRLMP
                     EREHPHGLSDRAFDSLFTKDKPVIFNFHAYASLIHKLTYRRTNHENIHVRGYKEKGNI
                     NTPLELAILNQVDRFNLAIDVIDRVPKLQATAAHTKEWLKDQIIDCVNYAHENGIDRQ
                     EIQEWKWPGEASQP"
     gene            3151..3351
                     /locus_tag="VFC15_17610"
     CDS             3151..3351
                     /locus_tag="VFC15_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12030.1"
                     /translation="MPQSPHDRVAELHNLAAHAHTAAAAAHGKGDHLTAHELSKQAHE
                     HSMNAYKHAKELIPISAESKKA"
     gene            3400..>4230
                     /locus_tag="VFC15_17615"
     CDS             3400..>4230
                     /locus_tag="VFC15_17615"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZM12031.1"
                     /translation="MNVFVLNSGSSSLKFQVIATDLDRIQQHQDQRICRGEVERLGGE
                     AIVTFQQGAGTKQKFTAPLRDIAAALDYLVRYIASDRSGVTEIKSSGDIHAVGHRVVH
                     GGELFSESALIDSKVLQDIEDCIDLAPLHNPNNIKGIVAARELFGKDVPQVAVFDTSF
                     HHSIPEQAFLYALPYHLYRRHRIRRYGFHGTSHRYVAFRYRALRELTRDQTNIISLHL
                     GNGCSETAIRHGNSVDTSMGMTPLEGLVMGTRSGDLDPAIVNLVATKEGMSPSEVDTL
                     L"
BASE COUNT          867 a         1254 c         1151 g          958 t
ORIGIN      
        1 cggccgtggt gtcgccatga agcacctgat ctcatccatg cgcatagcat tccgcgcgct
       61 gaaaatgaat aagctgcgct ccgcgctaac catgttagga attgtgatct tcggcttcgg
      121 cgcatcactg ttgatctcgc ggctagcgga ctgggcgaca tccgttggtc cgggcgcggt
      181 gttgctggca gtattgttct cggcgctggt gggtatcggc tttggctact atccggcgag
      241 aacggctgcg tacctggatc cgatcgaggc cttgcggaac gagtgagccg ttggtctggg
      301 tacggtgtct gattccactt tacatggaac gctcaccggt agctcagagc cgagcgaagc
      361 gtccgcttag ggctgagaag cctctcctgg ccacttccac tcctgtatct cctggcgatc
      421 aataccattc tcatgggcat aattcacgca gtcgataatc tggtctttta accattcctt
      481 cgtgtgagcg gcggtcgctt gcaacttcgg cacccgatcg atcacgtcga tcgcgagatt
      541 gaaccggtcc acctgattca gaatcgccaa ctccagaggt gtattgatgt tgcccttttc
      601 cttgtaccct cgcacgtgga tgttctcgtg attcgttctt cgataggtca gtttatggat
      661 cagcgaagca taggcatgga agttgaagat caccggtttg tccttggtga acaggctgtc
      721 gaaagcacgg tccgatagcc cgtgaggatg ttctctctcc ggcatcaacc ggaacagatc
      781 gaccacgttg atgaatcgaa tcttcagatc cgggcagcgc tctctcagaa tggcaaccgc
      841 ggccagcgac tccgaggtcg ggatgtcgcc tgcacaagcc atcaccacat ctggctcttg
      901 gccggcatca ttgctggcga agtcccagat cccgatgccc ttggtgcaat gcttcaccgc
      961 ttcgtccatt tccagatact gcagatgcag tgccttgtcc gccacgatga cattcacgta
     1021 gttcgtgctg cgcaggcagt gatcggtcac gctcagcagg caatttgcat ccggcggcag
     1081 atagattctc gtgacctccg gactcttgtt ggtcaccagg tcgaggaagc cagggtcctg
     1141 atgggtgaat ccgttgtggt cctgccgcca caccagagag gtgatcagca ggttgatgga
     1201 cgagataggt tccctccatc gcagttccag cttgctcttt tccagccact tcgcatgctg
     1261 gttgaacatt gagtcgatga tatggacgaa cgcttcgtag ctggaaaaga atccgtgtcg
     1321 tcctgtgagc acgtagcctt cgaaccagcc ttccagagtg tgctcgctca acatctccat
     1381 cacgcgccca tccgtcgcaa ggtcgccgcc atcggcatcc tcgggcttca tctccgccat
     1441 ccacgttttg ccagtggcct catagatcac ttgaagcttg ttggaggcgg tctcgtccgg
     1501 cccgaagacc cggaagctct tcatgtttct tcgcatcaca tcgcgcagaa agtgtgcgag
     1561 cgtatcggtt gaggaaacct cggtctgacc cggctgcgcg acgttcactg cgtattcgcg
     1621 gaagttaggc agatcgagcg gtttcctcag cgttccgccg tttgtgtgcg gattgccgct
     1681 gatgcgtctc gctccgcgcg gcgccgtatc cttcaattcg gcgatcagag tgccgttggc
     1741 accgaacaat tcttccggcc ggtaactccg catccacgat tccaccaact tcaaatgcgc
     1801 tggattggtg actggatcga gaatcggtat ctggtgcgcg cgccacgatc cttcgacctt
     1861 gtgcccgtcc aactccttgg gaccggtcca tcccttcggc gtgcgtaaga tgatcatcgg
     1921 ccaccgtggc cgctccggtg ccactcccac ccccacgctc ctcgccttct cctggatcgc
     1981 tcgaatctcg agtacgcagt gttccatcac gctggccatt tcctggtgca tgagcgccgg
     2041 atcactgcct tccactacgt acggcgtcca gccataccca acgaagagac tctccagttc
     2101 atccgatgga atcctcgcca gaatcgttgg gttcgcgatc ttgtaaccat tgaggtgcaa
     2161 caccggcaac acagcgccat cacagatggg attcaggaac ttgttcgagt gccacgacgt
     2221 cgccagtgga cccgtctccg cttcgccatc tcctacaact accgtcacga tcagatccgg
     2281 attgtcgaac gccgcgccga atccatgcga aaggctgtac cccagttccc ctccctcatg
     2341 gatcgaaccc ggcgtctcgg gtgtgcagtg acttccgatt ccgccgggaa acgagaattg
     2401 cttgaagaac ttctgcatgc caacggcatc ctcgcccatc tccggataaa tctccgaata
     2461 cactccctcg aggtagctgt tggcgagcgt cgctggtgct ccatggcccg gccctgaaac
     2521 atagatgagg tcgagatcgt acttcttgat gagccggttc agatgcaccc acgtgaaggt
     2581 ctgcccggga tccgaacccc agtgtcctag cagtctgttc ttgatgtgct cgggcttcag
     2641 aggttcgcgc agcagcggat tgtcccgcag gtacagcatg cctgcgcaaa gatagttaca
     2701 ggcacgccag taggcgtcca tcctccgcaa ttcgtcgggt gttagcggcg caattccgat
     2761 gtgagaggtg ttgttctgac gtgaaggagc ggtgatcgta ttcaagtgat tacctcgtac
     2821 aacggagtgg tttgggttct taatgccatc gtgagacgtc cctgttaaac gacggtgaat
     2881 accgccgact cagatgagcg agaccggcaa actccgcgtt cgtgattccc tcatggatgt
     2941 cacgcttcgc gctctggatg cacgatgcta tgccagacct agcgcatcgc acgcgtccgc
     3001 gggtcacata acatttgaag gaacatcgtg acgttgtgcg tcgccgaata ctgtgtcgaa
     3061 ttgcacagca cggcatctag cacagtggaa cactaaaaaa gcagcctgtg ttagcagtta
     3121 aaaggattca gttcgatcca ggagaattca atgccacaga gcccgcacga tcgagtcgca
     3181 gaacttcata acttggcagc tcacgcccat accgccgcag ccgccgccca cggcaagggg
     3241 gatcatttga ccgctcacga gctgagcaaa caggcgcacg agcactccat gaacgcttac
     3301 aaacacgcca aagagctcat cccgatttcc gccgagtcca aaaaagcata gggcgcagcg
     3361 acttgctggt acgatacaac cccaaagaga aagaccggcg tgaacgtttt tgtactcaat
     3421 tccgggagtt ccagcctgaa gtttcaggtc atcgccaccg atctggatcg tatccagcag
     3481 catcaggatc aacgcatctg ccgcggcgag gtggaacgcc tcggtgggga ggcgattgtc
     3541 acgttccagc agggcgccgg cacaaaacag aagttcaccg ctcctttgcg agacatagcg
     3601 gcggcgctgg attacctggt ccgctacatt gcctccgaca gatcgggggt cactgagatc
     3661 aagagttcgg gtgacattca cgcagttgga catcgcgttg tgcacggcgg cgagttgttc
     3721 tcggagtcgg ccctcatcga cagcaaggtc ttacaggata ttgaagactg catcgatctt
     3781 gcgccactgc acaatccgaa caacatcaag ggcattgtgg ctgcgcgaga actcttcggc
     3841 aaagacgttc cccaggtagc ggtattcgac acttcgtttc accattcgat tccggagcaa
     3901 gctttcttgt acgccctgcc gtatcacctc tatcggcgtc accgcatccg ccgctacggc
     3961 tttcatggaa cctcgcatcg ctacgttgcg tttcgctatc gcgctttgcg cgaactgacg
     4021 cgggaccaga ccaacatcat cagcctccat cttggaaacg gctgctcgga gaccgcaatc
     4081 cgccatggga attcggtgga tacctccatg ggcatgaccc cgctcgaggg tctggtgatg
     4141 ggcacgcgtt cgggagatct cgaccctgcg atcgtgaatc tcgtagcgac aaaagaaggg
     4201 atgtcgccgt cagaggtcga tacgcttctg
//
LOCUS       DASRKK010000730         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1346810, whole genome shotgun sequence.
ACCESSION   DASRKK010000730 DASRKK010000000
VERSION     DASRKK010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346810"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFC15_17620"
     CDS             complement(<1..949)
                     /locus_tag="VFC15_17620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZM12032.1"
                     /translation="MSILQHAVPGFLSVRSAFPITILVTADAAGQRLDQFLTAHIPDI
                     SRARVQQLLAEQKALVNGNVTKPSLRLRGGEQITILGDPEPPPLRAIAEHIPLDIIYE
                     DDDLAVINKPAGMMVHAGAGATDSERNRGTLVNALLHRFAKLSDIGGELRPGIVHRLD
                     KETSGLIIVAKNDVTHRKLGQQFSGRQVKKRYVALVHGWPAKDRGTINTEIGRDVVRR
                     IRMTTRGRGGRSAITHYEVRERIASQFGKFALVDVRIETGRTHQIRVHLSSIGHPVVG
                     DSLYRAPRELRRPTALRPASTKATSRGASQPEARQTPSVL"
     gene            complement(1194..2648)
                     /locus_tag="VFC15_17625"
     CDS             complement(1194..2648)
                     /locus_tag="VFC15_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12033.1"
                     /translation="MNPEGVEVVHTRYGRVFLLGLLIVIGPAVPFTFAESSSAQVSAT
                     GTIASHSTPKNELLPFAVTLRNDSASKLSSVRLVRTPENYELQKVCMLGSDEKVSCLD
                     GQQFAATGYPLTDAILPGQSFTMWGDLRPAATHKRETLTLIVGWKIANADPSASYPSS
                     LVVGLGENQVLGCLEGFWTAVWEAAKLLLIPIALAVIGVLLNLSMKKREERIEKREKL
                     RDEAQQKAEKLRDEARQDAERNRTARAETLKLMLPICHSYAGQYYLPLSIALAGMAKY
                     LRESIALEGKDDGLRDSKRKVAFYYHLLAWRRMSKITKAIGGFYLKDLRGEALVAECW
                     SRHKLAVAGGVEDQRYIATRAAIKRFTDDTDSYQAFEEQFENSAGEGYRDEDIQRAWT
                     RFQEWLNGKNKVKQDDKSTVQAQVALNLEAFSSILDWEANRPYQYWYDHAAKLTLNDA
                     TEKLLKEMLGNEVQFSAKERTEYFADVQKLQDTK"
     gene            complement(2820..>3180)
                     /locus_tag="VFC15_17630"
     CDS             complement(2820..>3180)
                     /locus_tag="VFC15_17630"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZM12034.1"
                     /translation="PWAVTFHNPLANEIVGTPLNVPLHPTQLYEMVVELCNCLFLVWL
                     LRRKKFEGEIMGTYMIIYGVARYFIEFFRGDPGRGEFMGFMSGTQAIALCLVIGGGLL
                     WMRRATLAPPVAVKAAR"
BASE COUNT          649 a          914 c          886 g          731 t
ORIGIN      
        1 cgagtacgct gggcgtctgc ctggcttccg gctggcttgc gccccgagag gtcgctttgg
       61 ttgaggccgg tcgcagagca gtaggtcggc gcagttctct cggcgcccgg tacagcgagt
      121 cgcccactac gggatggccg atcgacgaca ggtgcacgcg gatctgatgg gtgcggcccg
      181 tctcaatgcg aacgtccacc agcgcgaact tgccaaattg cgaggctatg cgctcgcgaa
      241 cttcataatg ggtgatggct gagcggcctc cgcggccacg ggtcgtcatg cgaatgcgcc
      301 gcaccacatc gcggccgatc tcagtattga tggtgccgcg gtctttcgcc ggccagccat
      361 gcactagcgc cacgtaacgc ttcttcacct gccgccccga aaattgctgg cccagcttgc
      421 gatgggtcac gtcgttcttg gcgacaatga tcagcccgct ggtctccttg tcgagacggt
      481 gcacaattcc cgggcgcaac tcgccgccta tatccgacag cttggcgaag cggtgcagca
      541 gggcgttgac cagcgtcccg cgattgcgtt cgctgtcggt ggctcctgct ccggcgtgca
      601 ccatcatccc cgctggcttg ttgatgacag ccaagtcgtc gtcttcgtag atgatgtcga
      661 gaggaatgtg ctctgcgatg gcgcgcaacg gcggaggttc gggatcgccg agaatggtga
      721 tttgttcgcc gccgcgcagc cgaagcgacg gcttagtgac attgccgttg accagggctt
      781 tctgttccgc caacaactgc tgcacgcgcg cccggctgat gtcggggata tgtgcggtga
      841 ggaattgatc gagccgttgg cccgccgcat cggcagtgac caggatcgtg atggggaagg
      901 cacttctcac ggaaagaaat ccggggacag catgttgcaa gatgctcata atcaataact
      961 tacattggat gagaaaacgt catgcgaacc ctgagccctc gttgcccaag acgcatcaaa
     1021 ataccagagg caatgaacac agccgaagca agatttcagc tttgagggct tgccaggcgg
     1081 ttcaaagtgt gcaaccatct taacggcagg agagcaccac aatggcgacc aagattcccg
     1141 actaagaccg cagacagact agtcttctct gaacgtgccc aagttctcgt tgctcacttg
     1201 gtgtcctgaa gtttctgtac gtccgcgaaa tattcggttc tttctttcgc cgaaaattga
     1261 acttcattac ccagcatttc tttcagtaac ttttcagttg catcattcag cgtcaactta
     1321 gcggcgtgat cgtaccagta ctgatacgga cgattggcct cccaatcgag aatcgaggag
     1381 aacgcctcga gattgagagc aacctgcgct tgcaccgtgc ttttatcgtc ctgtttcact
     1441 ttgtttttgc cgttcagcca ctcctggaac cgtgtccatg cccgttgaat gtcttcatcc
     1501 ctgtagcctt cccccgctga attctcaaat tgttcttcga aagcctgata gctgtcagtg
     1561 tcatctgtga atcgcttgat tgcagccctc gttgctatgt agcgttggtc ctcaacgccc
     1621 cccgcgacag ccagtttgtg tctactccag cactcggcta ccagagcttc accccgcagg
     1681 tccttcaagt agaatccccc aatcgccttg gtgatcttgg acattcttct ccaggcgaga
     1741 aggtggtagt aaaatgccac tttgcgtttt gagtcacgca aaccgtcgtc cttgccttcg
     1801 agggcaattg attctcgcaa gtatttggcc atacccgcga gggcaatgga taacggcagg
     1861 taatactggc ccgcatagct gtgacaaatg ggaagcatca gcttcagggt ctcggcccgc
     1921 gcggtccggt tcctttcggc atcttgccgg gcttcatctc tcagcttttc agctttttgc
     1981 tgggcttcat ctctcagctt ttctcgtttc tctattcttt cctctctctt tttcatggat
     2041 aggttcagga gaacacctat gacggcgagg gcgatgggga tcaagaggag cttggctgct
     2101 tcccatactg cggtccagaa gccttcaaga cagcctaata cttgattctc gccgaggccc
     2161 acgaccagtg aagagggata agatgccgag gggtctgcgt ttgcgatctt ccagccgacg
     2221 atgagcgtca gggtttcgcg tttatgggtc gcggcgggcc ttagatcgcc ccacattgtg
     2281 aagctttgtc ccgggaggat cgcgtcggtt agcggatacc ccgtagcggc gaattgttgg
     2341 ccatcgaggc aggacacttt ctcgtcactg cccaacatgc acactttctg taattcgtaa
     2401 ttctcgggag ttcgaaccaa gcgcacggag cttagtttag aagcggagtc attcctgaga
     2461 gtgaccgcaa acggcagcag ttcgtttttc ggggttgagt gggatgcaat cgttccggtc
     2521 gcggaaacct gtgccgaact cgattcggcg aaggtgaagg gaactgcagg accgatcacg
     2581 atcaagaggc cgagcagaaa aactcttcca taacgagtgt gcaccacttc cacgccctcg
     2641 ggattcataa ccttattcct gaccggctaa cacgcccaga cgacgcagaa tagcacgtgc
     2701 ccccttcaac gcacagtatc tcgtagttgt taggtgctcg cccttgcgcc ggggcacgat
     2761 cgagccgttt cacctggagc gtgaaatcag atgtcgtctt gtaaactgcg agctgaaaac
     2821 tacctcgccg ccttcaccgc cactggcggc gccagcgttg cgcggcgcat ccatagcagc
     2881 ccgccgccga tcacgaggca caacgctatc gcctgggttc ctgacatgaa tcccatgaac
     2941 tcgccgcgac cgggatcgcc gcggaagaac tcgatgaaat agcgagccac tccgtagatg
     3001 atcatgtatg tgcccatgat ctcgccttcg aacttcttac gccggaggag ccacaccagg
     3061 aacaggcaat tgcagagttc gaccaccatc tcgtaaagct gcgtgggatg cagaggcacg
     3121 ttgagcgggg tgccgacgat ctcattggcc aggggattgt gaaaggtcac ggcccagggc
//
LOCUS       DASRKK010000731         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_857394, whole genome shotgun sequence.
ACCESSION   DASRKK010000731 DASRKK010000000
VERSION     DASRKK010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857394"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFC15_17635"
     CDS             <1..763
                     /locus_tag="VFC15_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12035.1"
                     /translation="IERAQAFLLRTQNPNGGWPVFPGDDQEGGWVTSLAVTALRDLVP
                     AIPARLQGLHWVLNCAGKESNWFWKWKFRTADRHVRLDPDKYGWPWFPDTVSWVVPTA
                     FAVLALSQLPCSCGGLEPVPLRVERGVEMLLDRACPGGGWNAGNGVVYGTALAPHPDD
                     TAIALLALRDRTKEPIVQGSVDYLERVAATLTAPWSLAWAILALAAHRRPTTSLSSSL
                     VALRDLSSIEDTSTLAVVCLALDSPRALEAFGVTQ"
     gene            760..1743
                     /locus_tag="VFC15_17640"
     CDS             760..1743
                     /locus_tag="VFC15_17640"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZM12036.1"
                     /translation="MSLTRRELLIGSAALAAGTAATAPFLLPKYHTDRRQKRSRVAIL
                     HVEQYSQQLDQIIAAGLRLFPIDVRGKTVVLKPNLVDYIPGNAINTHPLLVLASAESF
                     RRLGAKSVLVAEGPGHQRDTHLVLSQSGHEQCLKGEKIPFVDLNRDELIRTPLRAGYT
                     GMKALWLPRTVLAADFLVSMPKIKTHHWSGVTLSMKNMFGVVPGARYGWPKNILHWKG
                     IQESILDLCATVPVHFVIADGIVAMEGNGPLNGTPRPMGKIVLADDPVAADAICARLM
                     GFEAGRLVHIREGSRFMGNAHPALIDEVGEIFRPPTTPFQVVQEFQSLRLQ"
     gene            2282..2358
                     /locus_tag="VFC15_17645"
     tRNA            2282..2358
                     /locus_tag="VFC15_17645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Asp,seq:gtc)
BASE COUNT          532 a          747 c          716 g          600 t
ORIGIN      
        1 tatcgaacgt gcgcaggctt ttcttttgcg tacccagaat cctaatggcg gttggccagt
       61 attccccgga gacgatcagg agggtggctg ggtcacttcg ctggcggtaa ctgctcttcg
      121 tgatctggtg ccggcgatac ctgcaaggtt gcagggcctt cattgggtgc taaactgcgc
      181 cggcaaggaa tcgaattggt tctggaaatg gaagttccgc accgccgatc gccatgttcg
      241 tcttgacccc gacaagtatg gctggccatg gtttccggac accgtcagtt gggttgttcc
      301 caccgcattc gcggttcttg cgctcagcca gcttccctgc tcgtgcggcg gtctggaacc
      361 agtccctctc cgtgttgagc gtggggtcga gatgttgctc gatcgtgcct gccctggagg
      421 aggatggaat gccggtaacg gcgttgtcta tggaaccgcc cttgcacctc atcccgacga
      481 cacggccatc gcgctcctgg cgctgcgtga tcgaacgaag gaacctattg tgcaaggctc
      541 cgtcgactac ttggaacgag ttgccgcaac tctaacggcg ccgtggagtc ttgcctgggc
      601 gattcttgct ctcgctgcgc accggcggcc cactacttcg ctaagcagtt ctcttgttgc
      661 gcttcgtgat ctctcaagca tcgaggacac cagcacgctg gctgtggtct gcctcgcact
      721 tgattcaccg cgcgcgttag aggcatttgg ggtaacgcaa tgagccttac tcgtagagaa
      781 ctcctgattg gaagcgcggc tctggctgcg ggcacagcgg caacggctcc ttttcttctg
      841 cccaaatatc atacggatcg acggcagaag cgatcccgag tagctatcct ccatgtcgag
      901 caatactccc aacagctcga tcagatcatt gccgcgggtc tccgactctt cccaatcgac
      961 gtccgaggca agacggtagt cctcaaaccc aatttggtcg attacattcc tggcaacgcc
     1021 atcaataccc atccattgct ggtccttgcg tcggccgaat cattccgccg cctgggcgcc
     1081 aagtctgtgt tggtagcaga ggggccagga catcaacgcg acacgcacct cgtgctttca
     1141 caaagtggtc atgaacagtg tctcaaaggc gagaagatcc cattcgtaga cctgaatcga
     1201 gacgaactaa ttcgcactcc attacgcgcc ggctatacgg gcatgaaggc tctctggctc
     1261 ccccgcactg tgctggcagc ggacttcctg gtttccatgc cgaagatcaa gacccatcac
     1321 tggtccgggg tcacgctgag catgaagaac atgttcggcg tagttccggg agcgcgatac
     1381 ggatggccga agaacatcct ccactggaaa ggtattcagg aaagcatcct cgatttatgc
     1441 gccaccgtcc ccgtccactt cgttatcgcg gacgggatcg tggcgatgga aggcaatgga
     1501 ccgctcaacg gcactccgcg gccgatgggg aagatcgtgc ttgcagacga tccggtggct
     1561 gctgacgcga tatgcgcgcg gttgatggga ttcgaagccg gacgactcgt gcacattcgc
     1621 gagggctcga gattcatggg aaacgctcat cctgctctga tcgacgaagt cggagaaatc
     1681 ttcaggcctc ccacgactcc tttccaagtg gtgcaggagt ttcaatctct tcgtcttcag
     1741 tgacgaacag gtgtcgctga gcgatggctc tccacacatc cgctcctcca caaagacccc
     1801 tggtcgatgg gtcgatggtg gcaaggtttg cagcgaaaaa cgcccccagc gaatatcaat
     1861 cgctgggggc gtgagataca tatggattta ggatgaccaa cttttacgat gcgtggcacc
     1921 tgcacgtaaa gtgtgccatt cgtacctaag caagcagcgg cgtatgtcgc attgtgctcc
     1981 gatggtgagc gctgaagttg tccgcatcac ggcgcatgac accctgcatg ttcacaacga
     2041 ttcgttgcgc aatgagtgcc gagtagatcc agcgcaagtg gcgatatgga aagatgcact
     2101 tctcccaagt ctcccgcccg ttgtgttaag ataaatttcg catgaacgca cagttattta
     2161 ttagctgttt tggcaggaag aagcgccact gcaggcaacc gcgagaaact atgcgctgta
     2221 tgcaaaagct gcaattccct tatttgactt gggtttgcgg tatcgcgttg attccagacg
     2281 cggggtcgta gttcagttgg ttagaacgct gccctgtcac ggcagaggcc gcgggttcga
     2341 gtcccgtcga ccccgccatc attccaaagg acttatggga tgatggcttg aaaatcccga
     2401 tccacaatcc aatccacaat tcattgaatc gcctccgttt tcattcccag ggctgccagg
     2461 aactcaccct gcgccgcccg tgtttcgtca ccgtcctcct gcgtataaag atccagcgtc
     2521 gtctgaatct tcgagtggcg gaggattccc tgcaccgttt tcggctctac cttagccttg
     2581 ttcaccagcc aatta
//
LOCUS       DASRKK010000732         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1445366, whole genome shotgun sequence.
ACCESSION   DASRKK010000732 DASRKK010000000
VERSION     DASRKK010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445366"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFC15_17650"
     CDS             <1..231
                     /locus_tag="VFC15_17650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZM12037.1"
                     /translation="FGKLRTASRQEPGCLMYIVHRHRTDSRRFFIYEQYRDDAALEAH
                     RRSPHFQEYAIDALKDIGERKEGELYSPLSGE"
     gene            complement(237..1313)
                     /locus_tag="VFC15_17655"
     CDS             complement(237..1313)
                     /locus_tag="VFC15_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM12038.1"
                     /translation="MNTKRATIVFLLALTLIVLAFAFVITYPFLKPFAFAIILAVVFH
                     PVHERVVRWTKRGPNVSSLLSTLLLIFLFGVPTFVISMLAANEALAAAHYLGRRSAEE
                     GGFALFIMTLADRPLRFVGRWVDLSKYDVNAIIASNVQKLSMWMFGFGAAFLSNVARF
                     ITDSLITFVVVFFLFREGKSWAYRLGALMPLSPQQVSRLYRNVSDTIVANVYGILSVG
                     AAQGILIGIALRIVGMPSAILLALAAAFASIIPVVGAALVWVPIAIYLLLTGSVWKGV
                     FLLIWGLVVVSSIDNVLRPWVVGGRVELHPLVLLFFIFGGVEAFGFLGLFLGPVVASV
                     LAALFDILREELKEAKTLPAAGDT"
     gene            1459..3225
                     /locus_tag="VFC15_17660"
     CDS             1459..3225
                     /locus_tag="VFC15_17660"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZM12039.1"
                     /translation="MHRWSKMFIPTLREAPADAEVASHKFLVRAGYIRQLAAGLYSFL
                     FLGQRSMLKITNIVREEMNRIGQDFHLPALHPREIWEASGRWQSMGDNMFRLKDRGGR
                     DLCLGMTAEEVMTEIARKELRSYKQLPQIWYQIQQKFRDEPRPKSGLLRVRQFTMKDS
                     YSFDLDASGLDVSYRKHYDTYCRIFDRCGLKYVAVEAHSGAMGGSQSHEFMVNTDAGE
                     DLVVSCADCGYAANLEKATSKLGPVEDLSPSGNGEPELVHTPGQKTIEDVARFLGVSP
                     KNTVKTLAYMMTEGADPKTDEPRSRPLVVLMRGDHMLNEAKLSGAIGGKEYRPMQEEE
                     IRELFHSPAGYLGPLNIEWAKDEANSTQPFLLVEEALHGRRNLIAGANKEEYHIRNLT
                     PGETFHPTASADLRTVVEGEGCSRCGSPLKISKAVEIGHIFKLGYKYSESMGARVLDE
                     HGKEVTPIMGSYGIGIERILTACIEQNHDQDGFWLPASIAPFDAVLSPLSTSDDLLNG
                     AVEIGARLEQAGFEVLLDDRDERPGVKFKDADLIGVPYRITVGSKKFTEGKVEVLVRE
                     SKQKEDVAIGAVVEHMKKLAQL"
BASE COUNT          799 a          986 c         1012 g          586 t
ORIGIN      
        1 ttcggcaaac ttcggaccgc ttcacggcaa gaaccgggct gcctgatgta catcgtccat
       61 cggcaccgga cggattcgcg gcgctttttc atctacgaac aatatcgcga cgatgccgct
      121 ttggaggctc accgcagatc cccgcatttc caggagtacg cgatcgatgc gctgaaggac
      181 atcggagaac gtaaagaggg cgaactctat tctccgcttt ccggcgagtg aggttttcac
      241 gtatcgcccg ccgcgggcaa ggtcttggcc tctttcagtt cctcgcgcag aatgtcgaag
      301 agcgccgcca gaactgacgc cacaaccggg cccagaaaca atcctaagaa cccgaacgct
      361 tctacgccgc cgaagataaa gaagagcagg accagcggat gtaactccac gcgcccgccc
      421 accacccagg ggcggaggac gttatcgata ctactcacca ccacgagccc ccagatcagc
      481 aggaacacgc ccttccagac ggaaccggtc agtaggagat agatagcgat ggggacccag
      541 accaaggcag ctccgacaac tgggataatg gaggcaaatg ctgcggccag ggccagaagt
      601 atcgcggagg gcatgcctac gatgcgcaac gcgatcccga tgaggatgcc ctgcgcagcc
      661 cctaccgaca ggattccgta aacattggcg acaatggtgt cggagacatt ccggtacagg
      721 cgggaaacct gctgcggaga tagtggcatc aatgctccca accggtaggc ccacgacttt
      781 ccctcgcgaa acaggaagaa cacgacaaca aaggtaatca gggaatcggt aatgaaacgc
      841 gccacattgc ttaggaaggc ggccccgaaa ccgaacatcc acatgcttag cttctgcacg
      901 ttggaggcga tgatcgcgtt gacgtcgtac ttcgagagat cgacccagcg ccccacaaag
      961 cggagtggac ggtccgccaa cgtcatgatg aacagcgcga atccgccctc ttcggcgctt
     1021 ctgcgaccca ggtagtgcgc cgccgccagg gcttcgttgg ccgccagcat ggagatgacg
     1081 aaggtaggca ccccgaacag gaaaatcagc agcagcgtgg acagcaacga cgacacgttg
     1141 ggccctcgtt ttgtccatcg caccacacgc tcgtgcaccg ggtggaagac aaccgcaaga
     1201 attatggcga acgcaaacgg cttcagaaag gggtacgtaa tgacaaacgc aaacgccaga
     1261 acgatcaggg tgagggcgag cagaaacacg atcgtagcgc gcttggtgtt cataggcgag
     1321 tccgttcaag ttgtaacacg aaccttccac gcgggacagc aaaggtgccc tgatgcgccc
     1381 tgatgcctca ggccggaaca agccctcggt acaatagtgt gtcatccaag ctcaaccaag
     1441 ggttccggaa aggcggtcat gcatcgctgg tccaagatgt tcattcccac gctgcgcgag
     1501 gccccagccg acgccgaggt cgccagccac aagtttcttg tccgcgccgg atacatccgg
     1561 cagcttgccg cgggcctgta ctccttcctc ttcctcggcc agcgctcgat gctcaagata
     1621 accaatatcg tccgtgaaga gatgaaccgc atcggacagg acttccatct tccagcgctg
     1681 catccgcggg agatctggga ggcaagcggc cgctggcagt ccatgggcga caacatgttt
     1741 cgcctgaaag accgcggtgg gcgcgacttg tgtctgggca tgaccgccga ggaggtcatg
     1801 accgagattg cgcgcaagga gctgcgcagc tacaagcagc ttccgcaaat ctggtatcag
     1861 atccaacaga agttccgcga cgaaccgcgg cccaagtccg gactgttgcg cgtgcggcag
     1921 ttcaccatga aagactcgta ttcgttcgac ctcgacgcct ccgggctcga tgtcagctat
     1981 cggaagcatt acgacaccta ctgccggatc ttcgaccgct gcgggttgaa gtacgtcgcg
     2041 gtcgaggccc attccggcgc gatgggcggc tcgcagtcgc acgagttcat ggtgaacacc
     2101 gacgcgggcg aggacttggt cgtgagctgt gctgactgcg gctatgcggc gaacctcgag
     2161 aaggcgacgt cgaaactcgg tcccgtcgaa gacctctcgc catccggcaa cggcgagccg
     2221 gaactcgtac atacaccggg gcaaaaaacg attgaagatg ttgcgcgctt cctcggcgtc
     2281 tcgccgaaga acacggtcaa gacgctggcg tacatgatga ccgagggcgc tgatccgaaa
     2341 acggacgaac cgcggtcgcg tccgctggtt gtgttgatgc gcggcgatca tatgctcaac
     2401 gaggcgaagc tctcgggtgc gatcggcggc aaagaatatc gcccgatgca ggaggaagag
     2461 attcgcgaac tgttccactc gcctgcggga tatcttgggc cgttgaacat cgaatgggca
     2521 aaggatgagg ccaactcgac acaaccgttc ctgctggtag aagaagcgct gcacgggcgg
     2581 aggaacctca tcgccggtgc aaataaggag gagtaccaca taaggaattt gactccgggc
     2641 gagaccttcc atccaaccgc gtcggccgac ctgcggaccg tcgtcgaagg cgaaggctgc
     2701 tcgcgctgcg gatcgccgct gaagatcagc aaggcggtcg agatcggcca catcttcaag
     2761 ctcggctaca agtattccga gtccatgggc gcacgggtgc tcgatgagca tggcaaggaa
     2821 gtcacgccga tcatgggcag ctacggtatc ggcatcgagc gcattctcac tgcctgcatc
     2881 gagcagaacc acgaccaaga tggcttttgg ctgccggcga gcatcgcgcc gttcgatgcg
     2941 gtcctctcgc ctttgagcac gtccgacgat ttactgaatg gcgccgtcga gatcggcgcc
     3001 agacttgagc aagccggatt cgaggtattg ctcgatgacc gggatgagcg gccgggagtg
     3061 aagttcaagg atgcggacct gatcggagtt ccctatcgaa tcacggttgg cagcaagaag
     3121 ttcaccgaag gcaaggtcga ggtactggtg cgcgagagca agcagaagga agatgttgca
     3181 atcggggcgg tcgtcgagca catgaagaag ctcgcgcaat tgtaggcaga gggttcacca
     3241 cagaggcaca gagaattacg ttactttgac cacctatcgc gcgcccacgt gcctgatacg
     3301 cttggaatca aggtgcaact ttatggctag ttcaaacact ctcacactgc atctggacgc
     3361 caagcttgcc aagaggctag aca
//
LOCUS       DASRKK010000733         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_663348, whole genome shotgun sequence.
ACCESSION   DASRKK010000733 DASRKK010000000
VERSION     DASRKK010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663348"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            880..1203
                     /locus_tag="VFC15_17665"
     CDS             880..1203
                     /locus_tag="VFC15_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12040.1"
                     /translation="MAAAAVQHRPIAAQYDCTRRLLCPHVVGYNEPGEWRVFCYQYGG
                     ETKSGPLPSGAEGIWRCLSLRKLSSVELLDSPWRTEPHARQRCVENIEIDADDYPGGD
                     PQNGQ"
     gene            complement(1264..2523)
                     /locus_tag="VFC15_17670"
     CDS             complement(1264..2523)
                     /locus_tag="VFC15_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HZM12041.1"
                     /translation="MANAAGHADRHLPLKNYCTGLLLPGERKSVEPMAARLAPHDVRR
                     THQSLHHFVADAPWNDQEMLEQVRRQVLPAMEKHGPVVAWIIDDTGFPKQGKHSVGVA
                     RQYCGELGKQDNCQAAVSLSVSTWNSSLPIAWRLYLPEVWCQDAERCQQAGVPKEVAF
                     QTKPEIALEQIRQAMEQHVPVGVVLADAGYGKGTQFRTDLTQLGLEYVVGIESNATVW
                     EPGQQPLPAPTRKPGRGAPPKRLQRNADHQPISVKQLALGLPPSAWKEIGWRQGSEET
                     LRSRFAAVRVRPAHRDYKRTEPYPEEWLLIEWPKKESEPTKYWLSTLPEKTSLKSLVK
                     IGKHRWIIERDYEELKQELGLGHYEGRGWRGFHHHATLCIAAYGFLISERNRFSPSAH
                     IGDLGLSAPGRPPDFRPRGSPRPTGTT"
     gene            complement(2662..>3200)
                     /locus_tag="VFC15_17675"
     CDS             complement(2662..>3200)
                     /locus_tag="VFC15_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZM12042.1"
                     /translation="KGRSGTMTHDYKRNGTTTLFAALETLEGKVVGECHHRHRHQEFL
                     KFLRRLNTEFPGDVPLHLIMDNYGTHKHAKVQSWLKRNPRFVPHFVPTSSSWMNLVER
                     WFGHLDNKAIRRGVFLSVADLKASIEAFLKAWNHDPKPFVWTATVESIQEKLTRCRRT
                     LEQIQSGCTSPKSRKRKQ"
BASE COUNT          624 a          924 c          957 g          695 t
ORIGIN      
        1 cactttacgg gcgctcggac ttcagaacag cagggcatca ttaataagaa tatgatgggc
       61 cggagtaatt cgattttggg gcggtcgtat ggtcgggcat ccggaagtag ttgagatgga
      121 cggtaagacg ccgactcgga ctttggcccc gccggggcct gagccccaca gcctcaccgg
      181 tagatgctcc ctcctggttg tcagtgccgg aataaaacgc ccgcatttct gccggttttg
      241 acgacggcag caacggcctc cggcggacgg ctggcgcgtc tttcctgttg acctcaagcg
      301 gcactcaatg cgccgaccgg gccacaagcg cccgatagcc ggccgggcga ccaagaacgt
      361 gacgcgggat ctccaacgcg aacccattcg ccactgctca tgctcaggct gaatgcgcgg
      421 ggaggctagc tgcttgttct gaacactcgc cggttgggaa ttcgacactc gctcgccacg
      481 gtcgccgaag taggcaccgg ctggccgacg tggtagtgtg cctcgacggc gcaactggtg
      541 gtgggtgccg tcgtcaaaac cggcgaaact gcggtttgac ggccgctatg ttgcccagct
      601 tgagggcgcg ttatggaccc tggagatgat tgaggccctg cggctgaagc ccagcgattc
      661 gctgatcccc cagttcacgc gtatcgtggc ggcggtggcc cactcaaagc gatcgacgat
      721 gcgctgcgat tgtgtctgga actggcctga ggccttccta gtattactgt gttataaata
      781 gcttgtgctc aaccacttgg cggagtgacg ctatcctaaa tggagatggg tgctccggcg
      841 tcgggtggcg gacaatctcc agaggaagtt caccgccttg tggctgcggc agcggttcaa
      901 caccggccaa tcgcggcgca atatgactgc acacgcaggc tcttgtgtcc gcatgtggtg
      961 ggttacaacg agccgggcga gtggcgagta ttctgttatc agtacggagg agaaaccaag
     1021 agcggaccgc tgccgagcgg cgctgagggt atctggcgct gtctgtcgct gaggaagctt
     1081 tccagcgtgg aactgttaga cagtccttgg cgaaccgaac cccatgcccg tcaacgttgc
     1141 gtggagaaca tcgagatcga cgccgacgac tatcctggcg gcgatccaca gaacgggcaa
     1201 tgaggtagtt gctgcagcag ttggcgcgcg atcacgatgc ggagggtggc gatcgagtga
     1261 ggattatgtc gttccggtcg gacgcggcga gccgcgcggg cggaagtccg gcggtcgccc
     1321 cggggctgat aatccaagat cgccgatgtg tgcagagggg gaaaaacggt tccgctcgct
     1381 gatcaggaac ccataagcag cgatgcacaa ggtggcgtga tggtgaaaac cgcgccaacc
     1441 ccgtccttca tagtgaccca atcccaactc ctgcttgagt tcctcataat cacgttcgat
     1501 gatccagcgg tgtttgccga tcttcaccag agacttcagc gaggttttct ccggcaaagt
     1561 ggatagccag tatttcgtgg gctccgattc tttcttcggc cattcgatca agagccattc
     1621 ttccggatag ggctcagtgc gcttataatc gcgatgtgca ggacgcacgc ggactgcggc
     1681 gaaccgcgag cgcagagttt cttcactgcc ttggcgccaa ccaatctcct tccatgccga
     1741 tggtggcaag ccgagggcca actgctttac cgaaatgggc tgatggtctg cattgcgctg
     1801 caggcgctta gggggtgcgc cccggcctgg ttttcgcgtt ggcgccggca agggttgttg
     1861 tcctggttcc cagactgtgg cgttggactc aatgcctact acatattcca aaccgagttg
     1921 ggtgaggtcc gtgcggaatt gtgtgccctt cccataaccc gcatctgcca gtaccactcc
     1981 caccggaacg tgctgttcca tcgcctgccg aatctgctcc agcgcaatct cgggcttggt
     2041 ttgaaaggca acctctttcg gaacgcccgc ttgttggcag cgttcggcat cctggcacca
     2101 cacttccggc aggtataacc gccacgcgat cggcaaactc gaattccacg tgctcaccga
     2161 caaactcacc gccgcctggc agttatcttg tttccccaac tcaccacagt actgtcgagc
     2221 gactcctacc gaatgctttc cctgtttggg aaatcccgtg tcgtctatga tccacgccac
     2281 tactggaccg tgcttctcca tggctgggag cacctgccgc cgcacctgct ccagcatctc
     2341 ctgatcgttc cacggtgcgt ccgccacgaa gtggtggagt gactgatgcg tacgacgaac
     2401 atcgtgcgga gccaagcgtg ccgccatcgg ctccacgctc ttgcgctctc cgggcaacaa
     2461 caatcccgtg cagtagttct tcaacggcaa gtgccgatcg gcatgccccg cggcgttagc
     2521 cagaccctcg atatatgccg cgaagcgcct ctgctgtggg cctggcgaag agaggcgtga
     2581 tcccacgaaa acaattctac aacaacttgt tcttttttaa cacagtaata ctagtgtagt
     2641 gtcttcgaaa taactagaca gtcattgttt ccgctttctg gacttggggc tggtgcagcc
     2701 ggactggatc tgttccaaag ttcgccggca gcgcgtgagc ttttcctgga tggattcgac
     2761 ggtcgcggtc cacacgaagg gtttgggatc gtgattccaa gccttgagaa acgcctcgat
     2821 ggacgccttc aggtcggcta cgctgaggaa gacgccgcgg cggatggcct tgttgtccag
     2881 gtggccgaac catctctcga caagattcat ccaactggag ctcgtgggga cgaaatgagg
     2941 cacgaaccga ggattccgtt tcagccagga ctgcactttc gcgtgcttgt gcgtcccgta
     3001 gttatccatg atgagatgca agggaacatc gccaggaaac tcggtgttga gccggcgcag
     3061 gaatttcaga aactcctggt gacggtgtct gtgatgacat tcgccgacca ccttaccttc
     3121 taaagtttcc agggcggcga acaaggtggt cgtgccgttg cgtttgtaat cgtgggtcat
     3181 cgtgccgctg cgccccttct
//
LOCUS       DASRKK010000734         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_173983, whole genome shotgun sequence.
ACCESSION   DASRKK010000734 DASRKK010000000
VERSION     DASRKK010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173983"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFC15_17680"
     CDS             <1..984
                     /locus_tag="VFC15_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZM12043.1"
                     /translation="HRIESHHLDSLPGKKLEYSAWQELWLNLESMLKPYSTVVMQYSP
                     NNQIPYISLVDGGMLELVRSFGKEIVTSANLVARFEATLTDAQIATHFKARDAVDKIT
                     AAFFPEMGRRVRNGGTHEFEMQQWVMEAFKRENLTTEDPPIVGVNAHSGDPHYEPTAA
                     TSAPIREGDFVLLDLWAKTNDPDSVYYDITWTGAIGSPSDKQRAVFEIVRDARNVGID
                     KVKQAFESKQKIQGWEVDEAVRGYIAGKGYGQYFIHRTGHSIGRDIHANGANLDNLET
                     KDDREILPNTCFSVEPGIYLPEFGVRSEINMMIRNGKGEVTGRIQNELVLV"
     gene            984..1415
                     /locus_tag="VFC15_17685"
     CDS             984..1415
                     /locus_tag="VFC15_17685"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HZM12044.1"
                     /translation="MANPQNTATVAQRSTKAAAKSDNSMAIIAPVAGWLFPGLGHFIQ
                     RRWIRGVLLMLAVFVMFFAGLGMQGKVYSLNTGDLLDILGFVGDLGAGALYFIARTMD
                     WGIGNIHRAVADYGTKYIVVAGLLNVIAAVDAHHIAIGKKQ"
     gene            1412..1594
                     /locus_tag="VFC15_17690"
     CDS             1412..1594
                     /locus_tag="VFC15_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12045.1"
                     /translation="MTFELSHFNAALLFALCASTVFGITQRNTPRDMMRYGAYCFALF
                     VGGVIVAAWAMWLIKH"
     gene            1649..2047
                     /locus_tag="VFC15_17695"
     CDS             1649..2047
                     /locus_tag="VFC15_17695"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZM12046.1"
                     /translation="MQVKSYQDLIAWQKAISLVTEIYRLTAQFPGHEIYGLTSQLRRA
                     SVSIPSNIAEGHGRATRGEFIQFLGHARGSLCEVQTQIFIAQRLGYIPQEREHTVIAM
                     TDELGRILNGLIASIEGRKLKPLASLAPNP"
BASE COUNT          487 a          645 c          631 g          424 t
ORIGIN      
        1 caccgcatcg aaagccacca tctcgactcg ctgcccggga agaagctgga atattcggca
       61 tggcaggagc tgtggctgaa cctcgagtcg atgctgaagc cctacagcac cgtggtgatg
      121 cagtactcgc cgaacaacca gattccctat atctcgctgg ttgacggtgg catgctggaa
      181 ttggtgcgca gcttcggcaa agagattgtt acctcggcga acctggtggc gcgctttgaa
      241 gccacattga ccgacgcgca gatcgcgacc cacttcaagg cccgcgatgc ggtggacaaa
      301 atcaccgccg cctttttccc ggagatggga cggcgcgtac gcaacggcgg cactcacgag
      361 ttcgaaatgc agcaatgggt catggaagcg ttcaagcggg agaacttgac caccgaagat
      421 ccgcccatcg tcggcgtcaa cgcacacagt ggcgacccgc attacgagcc tacggcagcg
      481 acgtccgccc cgattcgcga gggcgacttt gtgctgctgg atctctgggc gaagacgaac
      541 gatcctgata gcgtctacta cgacatcacc tggacgggcg cgatcggcag cccgagcgac
      601 aagcagcgcg cagtgttcga gatcgtgcgt gacgcccgca acgtcggcat cgacaaggtc
      661 aagcaggcct ttgagagcaa gcagaaaatt cagggctggg aagtggacga agcggtccgc
      721 ggctatatcg ccggcaaggg ttatgggcag tacttcatcc accgcacggg acattcgatc
      781 ggacgcgaca ttcacgccaa tggcgcaaac ctggacaacc ttgagaccaa agatgatcgc
      841 gagatcctgc ccaacacctg cttctcggtc gagccgggaa tctacctgcc ggaattcggg
      901 gtacgcagcg aaatcaatat gatgatccgc aacgggaagg gcgaagttac cggccgcatc
      961 cagaacgagt tggtgctggt ctgatggcga acccgcaaaa tacagctacg gtggcccagc
     1021 gttccaccaa ggctgctgct aagagcgaca attcgatggc gatcatcgcc ccggtcgctg
     1081 gatggctttt ccccggcctc ggacacttca ttcagcgccg ctggattcgc ggagtactgc
     1141 tgatgttagc ggtgttcgtg atgttcttcg ccgggctcgg catgcagggc aaggtctact
     1201 cgctcaacac gggtgacctg ctcgacattc tgggatttgt cggcgatttg ggcgcggggg
     1261 cgctttactt catcgcccga accatggact ggggaatcgg caacattcat cgcgccgtgg
     1321 ctgactacgg caccaagtac attgtggtgg caggattgct gaatgtgatt gccgcggttg
     1381 acgctcacca cattgcgatc ggcaagaaac aatgaccttc gaactctctc acttcaacgc
     1441 tgccctgctg ttcgcgctct gtgcctcgac ggttttcggc atcactcaac gcaatacccc
     1501 gcgcgacatg atgcgctacg gcgcgtactg ctttgcattg ttcgtgggcg gggtgatcgt
     1561 cgcagcctgg gccatgtggt tgattaaaca ctaaagatca ggggtcaggg gctaggggtc
     1621 aggtgctaga aaccctaagg acacgacaat gcaggtgaag agctatcagg atttgatcgc
     1681 ctggcagaag gccatctcgc tagtcacgga gatatatcgt cttaccgcac agtttccggg
     1741 acacgaaata tacggactca ccagccagtt gcggcgggcc tccgtatcca tcccgagtaa
     1801 tatcgccgag ggccatggcc gcgccacgag gggagaattc atccagtttc tcggtcacgc
     1861 gcgcggctcg ctgtgcgagg tacagaccca aatctttatc gcgcaaagac tcgggtacat
     1921 tcctcaggag cgggaacaca ctgtcatcgc catgaccgat gagctaggac ggatactcaa
     1981 cggactgatt gcttccatcg aaggcagaaa attgaagccc ttggcatcac tagcccctaa
     2041 cccctgaccc ctagcccctg ccttttcacc cttcccacac aaacgcatcg aacacccgca
     2101 gctcgacgaa gcccaaccgc ctatacagat ctaccgcccg atggttggcc tcggtcacgg
     2161 tcagcgacag caggctgaaa ttccggt
//
LOCUS       DASRKK010000735         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1348379, whole genome shotgun sequence.
ACCESSION   DASRKK010000735 DASRKK010000000
VERSION     DASRKK010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348379"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1369
                     /locus_tag="VFC15_17700"
     CDS             41..1369
                     /locus_tag="VFC15_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZM12047.1"
                     /translation="MSRPTVLVSSSVFFVFVFLTTAAIAGPRSPQSSNLGAQCVDCHS
                     KVTPNVVTDWKLSRHHEVGMACDTCHGSDHMTAADAAKAKIPTPETCGECHDVQVAQF
                     KKGKHAMAWASMEAMPTIHWQPMAMTEGMKGCGSCHKLGIKSPEQLAALQKQGARFGM
                     ASCDACHTRHTFSLQEARSPQACETCHMGFDHPQWEMYASSKHGVRNELKQLKIQSDT
                     SAAPTCQTCHMQEGNHEVRTGWGFLAVRLPLPDDPQWKADQVTILQALGVLDPSGKPT
                     ARLDVVKAADVARLDQASWQAERDKMLKTCNQCHSINFATQQLQYGDDMIKNADHLMA
                     QAIRIVADLYKDGLLPKPKNYAYPFPDLLTFHDAPTVIEQKLFVMFLEHRMRTFQGTF
                     HASPDYALWYGWSEMQRDLTEITELAAESRRTGHIATVDVKPVATQAVPK"
     gene            1830..>2057
                     /locus_tag="VFC15_17705"
     CDS             1830..>2057
                     /locus_tag="VFC15_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12048.1"
                     /translation="MKRIFLVILVVATFLAAGAIAQTADAPQAPHARKKRIAIFDFDY
                     ATVQSSSAAVFGTNVDIGKGIADLLVRDLVKD"
BASE COUNT          460 a          608 c          592 g          397 t
ORIGIN      
        1 cgttgatcga tgtccactac ccgctggtct ggaggtggtc atgtctcgtc ctaccgtact
       61 tgtgtcatcc tccgtctttt tcgtcttcgt gttcttgacg accgccgcaa ttgcgggacc
      121 gcgttcgccg caaagctcca accttggcgc acaatgtgtg gattgccata gcaaagttac
      181 tccaaacgtg gtaactgact ggaagctgag ccgccatcat gaggtgggca tggcttgcga
      241 tacctgtcac ggatcggacc acatgactgc agccgatgca gccaaagcga aaatccctac
      301 tcccgaaact tgtggcgagt gccatgacgt ccaagtggcg cagttcaaga agggcaagca
      361 cgcgatggcg tgggcctcga tggaagccat gccaaccatc cattggcagc cgatggcgat
      421 gaccgaaggg atgaagggtt gcggctcttg tcacaagctg ggaatcaagt ctccggaaca
      481 gcttgcggct ttgcaaaagc agggtgcgcg cttcggcatg gcatcgtgcg acgcgtgcca
      541 tacgcggcac actttctcat tgcaggaggc ccgctcgccg caggcgtgcg aaacctgtca
      601 catgggcttc gaccatcccc agtgggagat gtatgcctcc tccaagcatg gcgtgcgcaa
      661 tgagctgaag cagctcaaaa tccagagcga cacctcggcc gcgccaacct gccagacctg
      721 ccacatgcag gaaggcaacc acgaggtgcg caccgggtgg ggcttcctgg cggtgcggct
      781 accgctaccc gacgacccgc agtggaaagc cgaccaagtc accatcctgc aagcgctggg
      841 cgtgctggat cccagcggca aacctacggc acgcctcgat gtcgtcaagg ccgccgatgt
      901 cgcgcgcctc gatcaggctt cgtggcaggc cgagcgcgac aaaatgctga agacctgcaa
      961 ccagtgccat tccatcaatt tcgcaactca gcaactgcag tacggagacg acatgatcaa
     1021 gaatgccgat cacctgatgg cgcaggcgat tcgcatcgtc gccgatctct acaaagatgg
     1081 cctgttgccc aagccaaaga actacgccta tcccttccca gacctgctga ccttccacga
     1141 tgcgccaacg gtgatcgagc agaaactttt cgtcatgttc ctcgagcacc gcatgcggac
     1201 ctttcaaggc accttccacg ccagcccgga ctacgcttta tggtacggct ggagcgagat
     1261 gcaacgtgac ctgaccgaga tcacagaact agcggccgaa tcccggcgca ctggacacat
     1321 cgctaccgtt gacgtgaagc cggtggccac ccaagcggtg ccaaaataag gggctcgcgt
     1381 aggaatgatc ggaaggcaca gaggcaagga gaatgtccgt acctttggct tcatgggagg
     1441 aaaggcatgg actcgggtgg ggatcggtgg ccatatgacg cccccgacgc tcacagaaac
     1501 tacggcgtcg atcctccgta gggtccccgt cgcaagaagc tacacccaca cttccggact
     1561 gggcgctacc acactcttgt gcaacgacaa gcgcatcctt cgacccagtt tggatttttg
     1621 ttcaacttca ggaataacta tgctctagcc accgcaggcg gtgaacattc agcactggaa
     1681 aatctgcgcg ttagtgataa aatcgcgcca cacaggagcc gttcgagcga attcgaagtt
     1741 gacccagttg ggtggcgggc tgcgccgcag cgagtggcga tttatggatc ggcggaagaa
     1801 ccgttatttc acatgcaggg agactggtca tgaaacgaat cttcttggtg atactggttg
     1861 ttgcgacatt tcttgcggcc ggcgccatcg cgcaaaccgc cgatgccccg caggctccgc
     1921 acgcgcgtaa gaagcggatc gctatattcg atttcgacta tgcgacggtc cagagctctt
     1981 cggcggcggt cttcggcacc aatgtggaca tcggaaaggg tattgcagac ctgctggtga
     2041 gagacctggt taaggac
//
LOCUS       DASRKK010000736         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1250127, whole genome shotgun sequence.
ACCESSION   DASRKK010000736 DASRKK010000000
VERSION     DASRKK010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250127"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            541..951
                     /locus_tag="VFC15_17710"
     CDS             541..951
                     /locus_tag="VFC15_17710"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZM12049.1"
                     /translation="MPTAAEPASHLRQTLRALLADYQQRLDDLLPLRQLLKGSVYELK
                     TRCGKPSCHCVAPEGPLHSSTVLSWSERGKTCLRTLPPSDTAHLRHLAENHRRFRQDR
                     ATLVKLHNQTLLVIDRLERALLQPPPKPAAKRKS"
     gene            953..2644
                     /locus_tag="VFC15_17715"
     CDS             953..2644
                     /locus_tag="VFC15_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM12050.1"
                     /translation="MDLVAFHFDRSFVLEALRQGEIDYLEHVSEAAEADLFRHLIQRQ
                     VVQRLAETYPTPRKKEEVPVWIYLSSELSLKLHGAQSYHAFPRILRCGGLIEALGPEL
                     GGRRSLHPDTGDVTLACPGFNDKNDYDRQTPCDQDFLRKFARDTETDLLHGWFNRQVP
                     RCLRSLGLFDPEGLFIGDASYLFVPDNEHYEQSDLLWFDEHNHPVKAEEVDLRDKRYQ
                     QHRCYKLVSLIHVNRKLDFFFVVAARVVAGRRHECPILYELVEEVVRAVHKGFIKVLI
                     VDRGLIDGERMGHLKQKLAIDTIVPLRTNMDLYADAIGLTRLRGFAWEPYEPLVPPSP
                     PSTVTAKPLRVQKREAKRQRTLAERKAQAAASASSAASPPAPAPSAPQTMLGLARGLL
                     SWSQCPVPLTAVVNRERDQYGQTQDWVLVTTAERFTAAQIRSTYELRPAIEERHRQYK
                     CFWDLTRMHSCAFSLVVNQALLVLLAYTLVQAHLVLRQRQELNRGVWEYTRQLLSPSL
                     EVVAVYYRQRFCLLTLAEFGRILLEIPDPARAQLREKLKRIERQQYSLLENARPP"
BASE COUNT          553 a         1017 c          795 g          612 t
ORIGIN      
        1 cctgttgact gcggattcct cccgtgaaac gccgctcacg tcattagggc ctggcgatcc
       61 atcttgacga gacgtcaccg acctgccgac atgaactgca tcaccctctg gcaagtcggc
      121 cgcatttccg gagcccacac tcgacgcccg tcgatgaggc tgatcgaagc gattgtgccg
      181 cttgccttgg gaaccgctgt gctttggatt cggctaccga tatcaggtct taacgctcgc
      241 cgctccgttc gagacgcggc cagttgaaag atggccttca gcacacatgg ccttcatggc
      301 cttcctgcgg ctcagacagt aaattagcca tcaggaggcc cgcagggcct tccgtccggt
      361 aggagtcgct gaaagccgct ggagacgggg cccgcgagct tggccgcgtc tccgccgtca
      421 aggctccaca tctgatcacg gcgccgccaa ggattatttc tccctacctg cgtttttccc
      481 gcaaaaacat attgacttct ttatgttgtt atgatataca acataaaggg agaactgccc
      541 atgcccactg ccgccgagcc cgccagccat ctgcgccaga ccctgcgggc cctgttggct
      601 gactatcagc agcgcctcga tgacctgctg cctcttcgcc aactcctcaa gggctccgtc
      661 tatgaactca agacccgttg cggcaaacct tcctgccact gtgtcgcccc agaaggtccg
      721 cttcattctt ccaccgtgct ttcctggtcc gagcgcggca agacctgctt gcgtacgttg
      781 ccccccagtg ataccgccca tctccgccat ctcgccgaga accaccgtcg cttccgccag
      841 gaccgcgcca ccctcgtcaa actccacaac cagaccctgc tcgtgattga ccgtctcgaa
      901 cgcgccctgc tgcaaccgcc gcccaagccc gcggccaagc gcaaatctta aaatggacct
      961 cgttgccttc cacttcgacc gctccttcgt gctggaggct ctgcgccaag gcgagatcga
     1021 ctacctggag cacgtctccg aagccgccga ggcggacttg ttccgtcacc tcatccaacg
     1081 gcaagtcgtc caacgtctgg ccgaaaccta tcccacgccg cggaagaaag aggaagtgcc
     1141 ggtctggatt tacctgtcca gcgaactcag cctcaaactt cacggcgccc agagctatca
     1201 cgccttcccg cgcattctgc gctgcggtgg cctgatcgag gcgctgggac ccgagttggg
     1261 gggccgcaga agtctgcatc ccgacaccgg cgacgtgacg ctggcctgcc cggggttcaa
     1321 cgacaaaaac gactacgacc gccagacccc ctgcgaccag gacttcctcc gcaagtttgc
     1381 ccgcgacacc gagaccgacc ttttgcacgg ctggttcaat cgccaggtgc cgcgctgttt
     1441 acgctcgctg ggtctgttcg atccggaggg cttgttcatc ggagatgctt cctatctgtt
     1501 cgtccccgat aacgaacatt acgagcaatc ggacctgctg tggttcgatg aacacaacca
     1561 tccggtgaaa gccgaagagg tcgatctccg cgacaagcgc tatcaacagc accgctgtta
     1621 caaactggtg agcctgatcc acgtcaaccg caagctggac ttctttttcg tcgtggcggc
     1681 ccgcgtggtt gccggccggc ggcatgaatg ccccatcctc tatgaactgg tcgaagaggt
     1741 ggtgcgggcc gtgcacaaag ggttcatcaa agtgctgatc gtggaccgcg gtttgatcga
     1801 tggcgagcgc atggggcatc tcaaacagaa gttggcgatt gacaccatcg tgcccctgcg
     1861 caccaacatg gacctctacg ccgacgccat cggcctgacc agattgcgtg gtttcgcctg
     1921 ggagccctat gagccgcttg tgccgccgtc accaccctct acggtgacag ccaagccgct
     1981 ccgtgtgcag aagcgggagg ccaaacgcca gcggactttg gcggagcgca aggcacaggc
     2041 tgcggcttcc gcttcgtccg ctgcgtcacc gcccgcgccg gcgccgagcg cgccgcagac
     2101 catgctcggc ctcgctcgcg gtctgctgag ctggagccag tgcccggtgc cgctaacggc
     2161 ggtggtgaat cgggagcggg accaatacgg ccaaacgcaa gactgggttc tggtgaccac
     2221 tgccgaacgt ttcaccgccg cacagatccg ctccacctac gaactccgac cggccatcga
     2281 agagcgccac cgccagtata agtgcttctg ggatctcacg cgaatgcatt cctgtgcttt
     2341 ctcgctggtg gtgaatcagg cgctgttggt tctgctcgcc tacactctgg tgcaggctca
     2401 cctggtcctg cgtcagcgcc aggaactgaa tcgcggtgtc tgggaataca cccggcaact
     2461 gctctcgccc agcctggaag tggtggcggt ttattatcgc cagcggttct gtttactcac
     2521 cttggccgag ttcggccgca tcctgctgga gatccccgat ccggcccgcg cccaattgcg
     2581 cgaaaaactc aaacggatcg agcgccagca gtattctctc ctggaaaatg cgcggcctcc
     2641 ctaacctcca tctcgcacct caatcatttc gcccttccca tgcaccaccg atgcttcggc
     2701 ggagcgaatc ctattttctc cgatgacgca ctacgtgaac tctctggtcc cccatcgtca
     2761 tgcacattct ccctgccgcg cattcctgcc cgcctgaccg cgatgcgagc gtttctccgg
     2821 ttttccgctg gccaacaccc cagcggctcc tttcctgcct cacatgcgcc cccagtgtct
     2881 gaatcgcagg cactccgaaa gagttctgga taattctccc gattgggtct acaatctgct
     2941 caggtcaatt tcctggcgta ggttctttat accggca
//
LOCUS       DASRKK010000737         7725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1152352, whole genome shotgun sequence.
ACCESSION   DASRKK010000737 DASRKK010000000
VERSION     DASRKK010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152352"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFC15_17720"
     CDS             <1..745
                     /locus_tag="VFC15_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12051.1"
                     /translation="LGEGAAVASGGHSYNEVLPLMLARYCGPGLLGLGITALIAGFMS
                     GMAGNVSAFTTVWTYDIYRALINKDASDEHYVKMGRWCTIVGVLISVGTAYLVMSFLS
                     IMDYVQALFSFFIAPLFGTVVLGMLWKRATPKGGFWGLLAGTVSSIGMWAWVKLDPAA
                     LRYVALSPNARDMAENMYRALWSWIVCVSVTVVVSLLTKPKPDSELVGLVYGVTEIPH
                     EGDIALWQRPIFWAVVVAVVFVIFNILVW"
     gene            756..977
                     /locus_tag="VFC15_17725"
     CDS             756..977
                     /locus_tag="VFC15_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12052.1"
                     /translation="MRSGGGGISIWFFVGVSLLFNGALILGAGLWEVVYPPANPVVLF
                     HYHANVWWGAVLLMLGIFYSLRFLPRRAS"
     gene            1012..2430
                     /locus_tag="VFC15_17730"
     CDS             1012..2430
                     /locus_tag="VFC15_17730"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HZM12053.1"
                     /translation="MAKKKMTMGLIVGNRGFFPAHLANSGREEMIVVLQKAGMDVVVL
                     TPQESKCGAVTTREDVHRCVELFKTNRDHIDGIIVTLPNFGEERAIADTLRLANLQVP
                     VLVQATPDDAKKMTIAFRRDSFCGKMSACNNLKQYGIPYSITSLHTEAPDSPEFAKDL
                     EWFAGVCRVVNGLKNLRIGSIGARPTAFNTVRYSEKLLESQGISVETLDLSEVLGQIA
                     RMKDSDAAAQGKLAAIQQYVQTSGVPKEALLKMAKLAAVVEAWMHETEVAISAVQCWT
                     SIEENLGIVPCTVMSMMSNELLSSACEVDICGVLSMHALRLASETPSALLDWNNNYGS
                     NPDKAVCFHCSNLPKHFFRSVKMDFQEIIAGTVGKENTFGTCVGQVKSGAMSFARFST
                     DDISGHMRGYVGEGQFTDDPLETFGGAGVVEIPQMQKLLHFICERGFEHHVAANFSTV
                     AAVVHEATTRYSGWEMYWHGRN"
     gene            2594..3694
                     /locus_tag="VFC15_17735"
     CDS             2594..3694
                     /locus_tag="VFC15_17735"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HZM12054.1"
                     /translation="MKITLLLLVLMTMTVPSQSKVTKQPYGKAADGAAVELYTLQDGK
                     ITVRIMTYGGIIVSLEAPDRNGNATDVVLGFDSLDGYVTTGNKPYMGAIIGRYANRIA
                     GGTFTLDGKTYHVPKNDGDNALHGGTAGFNKKVWTGKEIKDGVELTYVSKNGEEGFPG
                     TLTASVRYTLSGNELRIEYSATTDADTVLNLTNHSYFNLKGQGNGNILDHQMKINATQ
                     YTPVNANLIPLWELAPVAGTPFDFLKSAVIGSRIDADNEQLKLAHGYDQNLVIDGGGQ
                     KIAIAAEAYEPTTGRVLEVLTDQPGIQFYTGNFLDGTVTGKGGKVYGRRDAFCLETQH
                     YPDSPNHPKFPTTELKPGEKFHSITIYRFSAR"
     gene            3928..4458
                     /locus_tag="VFC15_17740"
     CDS             3928..4458
                     /locus_tag="VFC15_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZM12055.1"
                     /translation="MPIAARKSNRRSYRALRWPTQLREKSTGERIHVAAVCFRLKGGE
                     LEFLLVRTRSGRWTFPKGGVDGDATNADAAAREAYEEAGVKGRIEFHPFHWYFHSKRD
                     RLRARRTVVSVHAHLCEVERLVSPKEGHRDPTWFSADKARRRLRENRKPELATEVIQV
                     IDRAVIRIRARSRRAA"
     gene            4730..6661
                     /locus_tag="VFC15_17745"
     CDS             4730..6661
                     /locus_tag="VFC15_17745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZM12056.1"
                     /translation="MDRKVEACTDFYEYANGAWRASNPIPASMDRWSRRWAAGESNKE
                     QLRTILDEESAKTNQPKGSVNQLVGDFYGACMDQSKVNQAGIKPVQPLLDDIGSMKDQ
                     ADVQKMIGRLGDIGVSVPFGLNAASDNHNPSQVIADISAGGLGLPDRDYYVKTEERFQ
                     QARAKYLIHVANMFKLDGYSDADAQKAATSVMQFETALAKATLDNVARRDPAATDHKT
                     TFAELQKLTPQFDWNGYFQAAKMAEIPLNVQEPKFLEEFNRQLTATSLPDWRTYLKWQ
                     LLHQSAPYLSQAFVDENFDFYQKTLAGVGELKPRWKQCVEATDLFLGEAVGQEYVARY
                     FPPEAKARAREMVKNILAAMSETVSQLDWMSPATKTKAEEKIATFNVKVGYPDKWKDY
                     SSVSISRGAYFEDVVAASTFLVTDDRSTIGKPVDRGRWGMTPPTSNAYYNPLLNEIVF
                     PAGILQPPAFSVKATDAVNYGAIGVVIGHEISHGFDDQGAQFDAQGRLNNWWTEEDLK
                     KFQAKTGCVAKQFDGYYIEPNIHHNGKLVLGESIGDLAGVKIAYLAFQNSQKGKPPAP
                     TIDGFTPDQQFFIAWGQFRGDATRPETQRLMVQGDPHPVAKYRVIGPLSNFPQFQQAF
                     SCKSDSPMVRPEDQRCVVW"
     gene            complement(6712..>7725)
                     /locus_tag="VFC15_17750"
     CDS             complement(6712..>7725)
                     /locus_tag="VFC15_17750"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12057.1"
                     /translation="AALGGGLLTVITAAIKLRVMDAHLPPFVEGFAAGTNYAVSFVLL
                     QIFGLVLATKQPAATAATFAGIIRESRGVERENKLTEFVSRITSTQLAAAIGNVAAVC
                     VGAIFFERLWLFVFSQSYLAHETATHVYETLHPLASGTAFYAAVTGVILWMAALAGGW
                     CENFAVYYRLPEAIAQHPLGSKVGGVRLKKFSRVLDRNLGGWSTSIVLGYLLGFTPVI
                     GRFFGLPLDVRHVTLSTGTLALAVAHFGAQSMGRTWFYQAVAGIGVIFILNLFVSFSI
                     AAYVGLRAYDVRPREQLEILRFLVWAGLKSPLRFIWPNYLRHSSNEEPDPSDERNRSS
                     ERH"
BASE COUNT         1716 a         2059 c         2338 g         1612 t
ORIGIN      
        1 gctgggcgag ggtgcagctg tggcatcagg cgggcatagc tacaacgaag ttctgcccct
       61 gatgctggcc cgctattgtg gcccgggcct cctcggcttg ggaattacag cgctgattgc
      121 tggatttatg tcgggaatgg caggtaatgt gagcgcgttc acgacggtct ggacctacga
      181 tatctaccgc gcgctgatca acaaggacgc cagcgatgag cactacgtca agatgggacg
      241 ctggtgcacg attgtcggag tgctgatttc ggtgggcacg gcctacctgg tgatgtcgtt
      301 cctgagcatc atggattacg tgcaggcgct gttcagcttt ttcattgcgc cgctgtttgg
      361 aaccgtagtt ctcggcatgc tgtggaagcg cgcgacgcca aagggcggat tctggggcct
      421 tctggcgggt acggtctcgt cgattggaat gtgggcgtgg gtgaaactgg atccagcggc
      481 attacgctat gtcgcgctat cgccgaacgc gcgggacatg gcggagaaca tgtatcgcgc
      541 cctgtggtca tggatcgttt gcgtcagcgt caccgtggtg gtcagtctct tgaccaagcc
      601 caaacccgat agcgaactgg tgggcctggt ctatggagtg acggagatac ctcacgaagg
      661 ggacattgct ttgtggcaac ggccaatctt ctgggcagtt gtggtggcgg tggtgtttgt
      721 gatcttcaac atcctggtct ggtaagggag cagttatgcg aagtggtggc ggcggcattt
      781 caatctggtt tttcgtcgga gtttcgctct tattcaatgg tgcgctgatc ctcggagccg
      841 gactatggga agtggtttat ccaccggcaa atccggtggt tttgttccac tatcacgcca
      901 atgtttggtg gggcgccgtt ctgctgatgc tcgggatctt ctacagtttg cgcttcttgc
      961 cccgacgcgc gtcgtagctg tcggggtctg actcatagca aagggactct tatggcgaaa
     1021 aagaaaatga ccatgggcct gatcgtgggc aaccgtgggt tcttcccagc tcacctggcg
     1081 aattcgggac gggaagaaat gattgtcgtc ttgcagaagg cgggcatgga tgtggtggtg
     1141 ttgacgccgc aggaatccaa gtgcggagcg gtgaccacgc gcgaggacgt tcatcgttgc
     1201 gtcgagctct tcaagacgaa ccgcgaccac atagacggaa tcattgtcac actcccgaac
     1261 tttggagaag aacgggccat tgccgacacg ctgaggctgg ccaatctgca ggtgcccgtg
     1321 ctggtgcagg cgacacctga cgatgccaag aagatgacca tcgccttccg ccgcgacagc
     1381 ttttgcggca agatgtcggc ttgcaacaat ctgaaacaat acggcattcc ctactccatc
     1441 acatcgcttc acaccgaagc gccagactcg ccagagttcg cgaaggacct cgagtggttt
     1501 gctggggttt gtcgcgtggt caatgggttg aagaacctcc gcatcggctc gattggcgcg
     1561 cgtcctacgg ccttcaatac ggttcgttac agcgagaaac tgctggagtc gcaaggtatc
     1621 tcggtcgaga cgcttgatct ttcggaggtg ctggggcaaa tcgcccgcat gaaggacagc
     1681 gacgccgccg cgcagggcaa actggcggcg atccagcagt atgtgcaaac cagcggcgtg
     1741 ccgaaagagg cgctgttgaa aatggcaaaa ctcgcggcgg tggtcgaggc ctggatgcac
     1801 gagaccgagg tcgccatcag tgcggtgcag tgttggacgt caatcgagga gaacctcggc
     1861 attgtgccct gtaccgtcat gagcatgatg agcaacgagc tgctttcgag cgcctgcgaa
     1921 gttgatatct gtggcgtgtt gagcatgcac gctctgcggc tggcctcgga aactccgtcg
     1981 gcgctgctgg attggaacaa caactacggg tcaaatccag ataaggcggt gtgctttcac
     2041 tgctcaaacc tgccgaaaca ttttttccgc agcgtgaaga tggattttca ggagatcatc
     2101 gccgggaccg ttggcaaaga aaatacattc ggcacctgcg tgggtcaggt gaaatcgggc
     2161 gccatgagct ttgcccgctt ttcgaccgac gatatttccg gccacatgcg cggctatgtc
     2221 ggcgaagggc aattcaccga cgatcccttg gagactttcg gcggcgcagg agtggtggaa
     2281 attccgcaga tgcagaagtt gttgcacttc atttgcgagc ggggattcga gcatcacgtg
     2341 gccgccaact tctcgaccgt cgcggccgtc gtgcacgagg caaccacccg ctactctgga
     2401 tgggagatgt actggcacgg acggaattag cgggcggttg tcgctgccat atttgggccg
     2461 tggctcgatc gggtgctctt tgcgttttgg tcggcgggga cgtttaatac ctagaagacg
     2521 tccgggctaa gtgcctggct tcggtaccga agttcgggaa cagctgtggg taaaggcaag
     2581 ggaaagaact cttatgaaga ttactcttct acttctggtg cttatgacca tgacagttcc
     2641 atcgcagtcg aaagtaacaa aacagccgta tggcaaggcc gcagatggcg ccgcggtcga
     2701 gttatacacg ttgcaagacg gcaagattac agttcgcatc atgacctacg gcggaatcat
     2761 cgtttctcta gaggcgcccg accgcaacgg taacgcgacg gacgtcgtgc taggcttcga
     2821 ttccctcgac ggctacgtca caaccggaaa caagccgtat atgggcgcaa tcatcgggcg
     2881 ctatgcaaac cgcatcgcgg gtggcacttt cactctcgat ggcaaaacct atcacgtgcc
     2941 caagaacgac ggggacaacg cgctgcatgg cggcacggct gggttcaaca agaaagtgtg
     3001 gactggcaaa gagatcaaag atggcgtcga gttgacctac gtcagcaaga acggcgaaga
     3061 gggattccct ggaacgctga ctgcgtctgt gcgctacacg ctgagcggaa atgaactgcg
     3121 cattgagtac tccgcgacga ccgatgcgga cactgtgctc aacttgacca accactccta
     3181 tttcaatctg aagggtcagg ggaacggaaa catcctcgat caccagatga agatcaatgc
     3241 cacgcaatac acacccgtga atgcgaacct gattcctctc tgggagttgg cgccggttgc
     3301 cgggactccg tttgacttcc ttaagtccgc ggttattggc tcgcggatcg acgccgacaa
     3361 tgagcagcta aaactggcac atggatatga ccagaatttg gtgatcgacg gcggtggtca
     3421 gaagattgca attgctgccg aagcctatga gccgaccacc ggacgcgtcc tcgaggtgct
     3481 aaccgatcag ccgggcatac agttctacac gggtaacttc ctggatggaa cggtgaccgg
     3541 caaaggcgga aaagtctacg gacgacgtga tgccttctgc ctggagaccc agcattatcc
     3601 ggattcgccc aaccatccga agtttcctac caccgaattg aagcccggag agaagttcca
     3661 ctcgattacg atctaccggt tctccgctcg ctagagcggc tgcgggagtg gcgacacttt
     3721 ctgtgatcgc tgctcctgca gtcagggatt cggcgaccct ccgcactttt gctgtgttgt
     3781 gtccctttgg aattccgggc agccattcac tagatgggta cggccccagc aacgttaacc
     3841 gatttttaac agaggaacgg gccgacttag gggatactac ttagtcagca agctaacctg
     3901 aacgaggcag cctgctggag cgacctgatg cccatagcag ccagaaagtc caatcgccga
     3961 agctaccgcg ccttgcgatg gccaacgcag ttgcgcgaga aatccacagg cgaacgcatt
     4021 cacgtggctg cagtttgctt tcggctgaag ggtggcgaat tggagttctt gctggtgcgc
     4081 actcgaagtg gccgctggac gtttcccaaa ggtggggttg acggagatgc aaccaatgcc
     4141 gatgccgcag cgcgcgaggc ttatgaagag gccggcgtca aaggcaggat cgagttccac
     4201 ccgtttcact ggtactttca ctcgaagcgc gaccgcttgc gcgcgcggcg gacggttgtt
     4261 tccgtccatg cgcatctctg tgaagtcgaa cgattagttt cgcccaagga gggtcatcgc
     4321 gacccaacct ggtttagcgc cgacaaggcg aggcggcggt tgcgggagaa ccgaaaaccg
     4381 gaattagcca ctgaagtaat acaagtcatc gaccgcgccg ttatccgcat acgagctcgc
     4441 tcccggcgag cggcttgagg cgcatcccac ctcgataatc gaaccactca gtgccgcgat
     4501 ctgcagcgga tttccgtcag attgcgttcc gaaacccgca cttggtcacg aacctgaact
     4561 tgcgggtgac cttagctcca cgttgtcact gcaactgagt acaatggcgc tggtctgtaa
     4621 tcttgctttc atgggggaaa ccatgcgggt tgttagcgcc gtgctgagtg cagttttgtg
     4681 ggtgggctcc gctttggggc aagcgcaaca gggagttcaa atcggagaca tggatcggaa
     4741 ggtcgaagcc tgtacggatt tttacgagta cgcgaacggc gcatggcgcg ccagtaatcc
     4801 aattccggcg tcgatggaca gatggagccg tcgttgggcc gcaggcgaat cgaacaagga
     4861 acaacttcgc accatcctgg atgaggaatc tgcgaagacc aaccaaccca aaggaagcgt
     4921 gaatcagttg gtaggcgatt tctacggcgc ctgcatggac caatcgaagg tcaaccaggc
     4981 tgggatcaag ccagttcagc cgctgcttga cgacattggc tccatgaaag accaggcaga
     5041 tgtgcagaaa atgattggac gcttggggga catcggcgtc tccgtcccct ttgggctgaa
     5101 cgcggcttct gacaaccaca atccgtcaca agtgattgcc gacatatcgg caggcgggct
     5161 ggggcttccg gaccgcgact attacgtgaa gactgaagag cgctttcaac aggcgcgggc
     5221 gaagtatctg attcacgtcg caaatatgtt caagctggat ggatacagcg atgctgacgc
     5281 ccagaaagct gccacctcgg taatgcagtt tgagaccgcg ctggcaaagg cgacgctcga
     5341 caatgtcgca cgtcgtgatc cagcggctac cgaccataag acgacgttcg ccgaattgca
     5401 gaagctaacg ccgcaattcg attggaatgg gtatttccaa gcggcgaaaa tggcggagat
     5461 cccgttaaac gtgcaggaac ccaagttcct cgaggagttc aatcggcagc taacagcgac
     5521 ctctttgccg gattggagga cttatctgaa gtggcaactg cttcaccaat ccgctccgta
     5581 tctgtcgcag gcttttgtcg atgagaactt cgatttttat cagaagaccc ttgccggagt
     5641 tggtgaactg aagccgcgtt ggaaacagtg cgtggaagcc accgacctat ttctgggtga
     5701 ggcggttggt caggaatacg ttgcccgata ctttccgccc gaagccaagg cccgcgcacg
     5761 ggagatggtg aagaacatcc tggcggcgat gagcgagacg gtcagtcaac tcgattggat
     5821 gtcacccgcc acaaagacca aggcggagga gaagatcgcg accttcaacg ttaaggttgg
     5881 atatcccgac aagtggaagg actacagcag tgtctccatt tcgcgtgggg catacttcga
     5941 ggacgtggtt gcagcgtcga cgtttctggt gaccgacgac cgttccacca tcggcaagcc
     6001 ggtcgatcgt ggacgttggg gcatgacgcc tccaacttcg aacgcctact acaatcccct
     6061 gctgaacgag atcgtcttcc cggcgggaat cctgcagcca ccggcgttca gcgtgaaggc
     6121 gacggatgca gtgaactatg gcgcgatcgg agttgtgatc ggacacgaaa tcagccacgg
     6181 ctttgacgat cagggagcgc aatttgatgc acaaggtcgg ctgaataatt ggtggacgga
     6241 agaagatctt aagaaatttc aagcgaagac cgggtgtgtg gcgaagcagt tcgacggtta
     6301 ctacattgag cccaacattc accacaatgg caagctggtt cttggcgagt cgattggcga
     6361 cctggcggga gtaaagatcg cctatcttgc attccagaat tcgcaaaaag ggaagccgcc
     6421 tgctccaacc atcgacggat tcacgccgga ccagcagttc ttcattgcgt ggggacagtt
     6481 ccggggagac gctactcgcc ccgagacgca gcgtctgatg gtgcagggcg atccccatcc
     6541 agtggcgaaa taccgggtga tcggaccact ttctaacttt ccgcagttcc agcaggcgtt
     6601 ttcctgcaag tcggatagtc cgatggtccg gcctgaagac cagcgatgtg ttgtctggta
     6661 agttgagcac aaacttgagt tgcgggcgcc aggcgatcaa aacgcactgg cctagtggcg
     6721 ctcgctgctt ctatttcgct cgtcggatgg gtcgggctct tcgttgcttg agtggcggag
     6781 gtagttcggc caaatgaacc gtaacgggga tttcagtcct gcccatacca ggaatcgcag
     6841 gatctccaat tgctcccgcg gtcgcacatc ataggcgcga agtccgacgt aggcggcgat
     6901 gctgaagctc acaaacaggt tgaggatgaa gatgacgccg atccctgcga ccgcctggta
     6961 aaaccacgtg cggcccatgc tttgggcccc gaagtgagca acggccaggg cgagggtacc
     7021 agtcgataat gtaacgtgac gcacgtcgag tggtagtcca aagaagcgcc cgatcacggg
     7081 agtgaatcca agaagatatc cgagcacgat gctggtgctc cagccgccca aattcctgtc
     7141 caacacgcgg gaaaacttct tcagccgaac tccgccgact ttcgaaccca gcggatgctg
     7201 cgcaatcgct tcgggtaacc gatagtaaac ggcgaaattc tcgcaccatc caccggcgag
     7261 tgccgccatc cacaagatga cgcctgtgac cgcggcgtaa aatgcagttc ccgaggccag
     7321 tggatgcagt gtttcgtaaa cgtgggttgc ggtctcgtgt gccaggtaag attgtgaaaa
     7381 gacaaacagc cacagtcgct cgaagaaaat tgcgcccacg cacacggcgg caacattgcc
     7441 gatggccgct gccaactgtg tgctggtgat gcgtgagacg aattccgtaa gtttgttctc
     7501 gcgctccacg ccccggctct cgcgaataat cccggcaaaa gttgcggcgg tcgccgccgg
     7561 ctgcttggtg gcaagaacga gtccgaaaat ctgaagcagc acgaagctga ccgcatagtt
     7621 ggttccggcg gcgaatccct ccacgaacgg tggcaaatgg gcgtccatga cccgcagttt
     7681 aatggcggcg gttatgacgg tcagcaatcc gccacccaac gcggc
//
LOCUS       DASRKK010000738         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_371199, whole genome shotgun sequence.
ACCESSION   DASRKK010000738 DASRKK010000000
VERSION     DASRKK010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371199"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFC15_17755"
     CDS             <1..584
                     /locus_tag="VFC15_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZM12058.1"
                     /translation="FLAQATPEDKLEYIKKEQSAGRLVAMTGDGTNDAPALAQADVGL
                     AMNTGTVAAKEAGNMVDLDSNPTKLIEVVAIGKQLLITRGALTTFSIANDVAKYFAII
                     PAMFMATYPVLGHMNIMGLHTPQSAVLSAVIFNALIIIALVPLALRGVKYRPSDAASL
                     LRRNLLIYGLGGIIVPFPGIWLIDQLLAVLHLA"
     gene            598..1335
                     /locus_tag="VFC15_17760"
     CDS             598..1335
                     /locus_tag="VFC15_17760"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HZM12059.1"
                     /translation="MWSELLPGLRISLFATVLLGVVYPLSVTGICQVLFPHQANGSLI
                     IAGGKVVGSELIGQNFSKPEYFHPRPSSAGSGYDATASSGSNLGSTSAKLVRGTTKMD
                     DKKNEVVDFDGINLRIVHFCLDNDIPYESSAPLDRFKDATGGLDDVKLIKAFNDDKAP
                     LLFKAKAPIPADAVTASGSGLDPHISPDSARAQAARVAKARGIRVDQANQLLAQFTEG
                     PDLGLLGEPRVNVLKLNLAMDQQFPRK"
     gene            1475..2710
                     /locus_tag="VFC15_17765"
     CDS             1475..2710
                     /locus_tag="VFC15_17765"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM12060.1"
                     /translation="MKTLLFFVLAVPFAFCQETQNPPSPQTQPVPADTTAQAPPAAPA
                     TPPPAPAALPTPSITGPLQGLPPAIFDAGPFGKLAVNGFLSGMGLWQSNHIPGDDSTQ
                     AALSNGQVFLQKTDGWFQFYLQAGAYNIPALGVPFLATDKTVTDFYGPLPVAFLKLQA
                     GKNTSFLIGALPTLMGAESTFTFQNMNIERGLLWNQENAVNRGIQVNQTMGKFTASLS
                     WNDGFYSNRYSWLSGSLTYASGPHSLAFVGMGNLAQTKFQSLATPVQNNGTMYAVLYT
                     FNKAPWIIQPYWQYTSIPTNPSIGVVKGATTNAAAILVSRTFKHGFSLPARFEYIKSS
                     GSASDQSVNLMYGPGSSGTSITVTPTYQHGGFFVRGELAYVHAGSIAPGSAFGSDGTL
                     QHQTRAMAEFGFIFGNNIR"
     gene            2768..4186
                     /locus_tag="VFC15_17770"
     CDS             2768..4186
                     /locus_tag="VFC15_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12061.1"
                     /translation="MQDSELSFVIPTHRLRDVGETVEQYDEHFWRNGHSVRIIVFDDS
                     TTPNQEKYYPLLEQTHTHQDVYYVGPREKEQFLGYLNGRLRDQRLGALVKNLFRPSYG
                     GNRNFTLMYTLGGLMVSSDDDMRPFSLIEYSRESLGAGEVSRGRLHKADQNGYTRKSF
                     DVMAAFLDVLGRPVSEVPDNYERGELLVDTAMDLETNATKGLAHENSLILERGTVPAD
                     SRVKIAQTFRSGTNDVDAIDFVDMFLEDEGQTNLDTLNDVYVLVNFRPVITNKNWRMD
                     CGVAGYDNTFGLPPFFPTRLRFEDYIYRLWVQQDGVAAAHVDAAQNHSKSNYMRNPPA
                     AEIFNEEVSNLLKRKIKASVTSLDELSIAFDYDGHVTVEDASEILDKITALHSRALLA
                     ADAARNADRAQALRIFAANLAKAFYGFEPDFFQQNLTRIADDVVGVIKASIQLWPTLV
                     EIAYFQKRRSGLPQARVENHRK"
     gene            4192..5280
                     /locus_tag="VFC15_17775"
     CDS             4192..5280
                     /locus_tag="VFC15_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM12062.1"
                     /translation="MRIAQVAPLYESVPPRLYGGTERVVSFLTEELVRLGHEVTLFAS
                     GDSKTMATLVAPCERALWRDPACRETLPQHVRLMELVFRKASQFDVIHFHNDYLHFPL
                     LRHNPTPTVTTLHGRLHLHDVQPLFREFAEVPLVSISEDQRRPLPGANWQATVYHGLP
                     RETHTFQDEPGEYLVFVGRVSPEKRLDRAIEIARRAGRKLKVAAKIDDEDRGYYQQSI
                     EPLLQASRSFVEFVGEVGGSAKDELLGGACALLFPIDWPEPFGLVMIEALACGTPVIA
                     WRNGSVPEILENGKTGFVVETVAEAVEAVRKIESLDRLVCRRAFEERFDSVRMARNYV
                     RVYERLIHESPSRILESASATMPIGSRV"
     gene            5285..7468
                     /locus_tag="VFC15_17780"
     CDS             5285..7468
                     /locus_tag="VFC15_17780"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZM12063.1"
                     /translation="MIQESRRSEGKPGEDAHHILADSSLTDECIRVLKHGDTFALFDQ
                     YGDIKPSKAGEQGVYHDGTRFLSRLILELDGRRPFFLSSTVRDDNDQLTVALTNPDLC
                     RDGVVYLPLGTLHLAVKKFLWQGALHQEIQIQNHGVQFADATVTLQVGADFADIYEVR
                     GLKREARGEDLEPIASLDRVTLRYRGLDLTIRRTVLQFTPSPNRLTAGRAEFTVALAP
                     KETASFQLVVGFESGPEERPLVAFDQARVEAKADLGRHKAQLCRIESVNGQFNALVQR
                     AASDLHMMITTLPSGPYPYAGVPWFNAPFGRDGIITALECLWFHPSLARGVLAYLAST
                     QATEVIPGQDAEPGKILHETRNGEMATLGEMPFGRYYGTVDATPLFVVLAGAYFERTG
                     DTRFVEGIWPNIEAALLWMDRYGDRDGDGFLEYDRQSSDGLLHQGWKDSDDAIFHADG
                     SPAQGPIAVCEVQGYAYAAWRAGATMASALGRAGQAEDFVSQAVMLKTRFDRMFWCEE
                     LSTYGLALDGDKRLCRVRTSNAGQCLFSGIATTDRAERVAKTLLAPESFSGWGIRSVA
                     ASEQRYNPMGYHTGSVWPHDNALIAYGLARYGFAGEALRVFEGLFDAGMYFDLHRIPE
                     LFCGFPRDPGEGPVLYPVACAPQAWSAGAVFLLLQACLGLRVGGSKPQLSFVRPTLPA
                     FLGETRILNLQVANAEVDLLLTRHDRDVAVKVLRREGDVEIMVLM"
     gene            complement(7594..>7836)
                     /locus_tag="VFC15_17785"
     CDS             complement(7594..>7836)
                     /locus_tag="VFC15_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12064.1"
                     /translation="PPGRDFFSLNFAQELLGRLPAGLTRHAAILGFECMCSRQTSQHK
                     HFRSGKTDHESGDVTKVATAGGDALAWPRIQCRQER"
BASE COUNT         1587 a         2315 c         2346 g         1588 t
ORIGIN      
        1 atttcctggc gcaggcgacg ccggaagaca agctggaata catcaagaag gagcagtccg
       61 cgggccggct ggtcgcgatg accggtgacg gcaccaacga cgctccggcg ctggcccagg
      121 ccgatgttgg cctggccatg aacaccggta cggtggcagc caaagaagcc gggaacatgg
      181 tggatctcga cagcaaccct accaagctga ttgaggtggt tgccatcgga aagcagttgc
      241 tgatcacgcg cggagcgctc accaccttct cgatcgctaa cgacgtagcc aagtacttcg
      301 caatcatccc cgcgatgttc atggcgacct atccggtcct cgggcacatg aatatcatgg
      361 gactgcatac gcctcagagc gcggtgctct cggcggtgat cttcaacgcc ttgatcatca
      421 tcgccttggt tccgctcgcg ttgcgtggcg tcaaataccg gccgagcgac gccgcgagcc
      481 tgctgcgccg gaacctgctg atctatggct tgggcggcat catcgtgccg ttcccgggta
      541 tctggctgat cgatcaactg ctcgcggttt tgcaccttgc ctaaaggaga gaatttcatg
      601 tggtcagaat tgttgccggg acttcgaatc agtcttttcg ccacggtctt gttgggcgtg
      661 gtttatcctt tgagcgtcac gggcatttgc caggtcttgt ttccgcatca ggccaatggg
      721 agcctgatta tagcgggagg caaggtggtc ggctcggaac tcatcggcca gaacttcagc
      781 aaacccgagt attttcaccc gcggccgtca tcggccggta gcgggtacga tgcgaccgcc
      841 agtagcggtt cgaaccttgg atcgacgagc gccaagctcg ttcgcggcac gaccaaaatg
      901 gacgacaaga agaacgaggt ggtcgatttc gatggcatca accttcgcat cgtgcacttc
      961 tgccttgaca acgacattcc ctatgaatca tcggcgccgc tggaccggtt taaagatgcc
     1021 accggtggcc tcgatgatgt gaagctgatc aaggccttca atgacgacaa agcgccgttg
     1081 ttgttcaagg cgaaagcgcc gatccccgcc gatgcagtaa cagcgtcggg aagcggcctc
     1141 gatccgcaca tcagtccgga ttccgcgcgg gcgcaggccg ctcgcgtcgc caaggctcgt
     1201 ggcatccgag tggaccaggc taaccaattg cttgcccagt ttaccgaagg gcccgatctg
     1261 ggccttctcg gtgagccccg ggtcaacgtg ctcaagctga atctcgcgat ggaccagcag
     1321 ttcccgcgca agtaaagagt caactcagtt cggaagttaa tgccctcgcg ggggggccag
     1381 ttactcctag aaggagttct gtgtgtaggt ggctacccgc gaggacgctt tttcgatgca
     1441 tcccagacca atacgtgtct ttaggagaca gatcatgaaa accctgttgt tttttgtcct
     1501 tgctgtgccc tttgcattct gtcaggagac ccagaacccg ccgtcgccgc aaactcagcc
     1561 ggtgccggct gatacgactg cacaggctcc gccggccgcg ccggcgaccc cgcctccagc
     1621 cccggccgcg ctacccactc cttccataac gggtccgctg caggggctcc ccccagctat
     1681 cttcgacgcc ggacctttcg gcaagctcgc tgtgaacggc tttctcagcg gcatgggcct
     1741 gtggcagagc aatcacatcc cgggtgacga ctccactcaa gcggctctaa gtaacgggca
     1801 ggtgttcctt caaaagaccg acggttggtt tcagttctac ctgcaggcgg gcgcctataa
     1861 tattccggcc ctaggggtgc cgttcctcgc caccgacaaa acggtgaccg acttttatgg
     1921 cccgctgccc gtcgcctttc tgaagctgca ggcggggaag aacacctcct ttctgatcgg
     1981 tgccctaccg acactgatgg gcgccgagtc aacgttcacc ttccaaaaca tgaacatcga
     2041 gcgcggcctg ttgtggaacc aggaaaacgc cgtcaatcgc ggaattcaag tgaatcagac
     2101 gatgggcaaa ttcactgcct cattgagttg gaacgacggg ttctattcca accgctacag
     2161 ttggctgagc ggctccttga cttatgccag cggaccccac agtttggcat tcgtaggcat
     2221 gggaaacctg gcgcagacga agtttcagag tctggccacg ccggtgcaaa acaacggcac
     2281 gatgtacgcc gtgctgtaca cgttcaacaa agcgccctgg atcatccagc cctactggca
     2341 atacaccagt attccaacca acccgagtat cggcgttgtg aagggggcaa cgaccaatgc
     2401 ggccgcgatc ctggtgagtc ggaccttcaa acacggcttc tcgctccccg cccgctttga
     2461 gtacatcaaa agctcgggtt cggcctccga tcaatcggtc aatctgatgt acggcccggg
     2521 cagctccggc acgtcgatca ccgtgacgcc cacctatcag cacggcggct tcttcgtccg
     2581 cggcgaactt gcctatgttc acgcgggcag catagctccg ggcagcgcgt tcggcagcga
     2641 cggcactctc caacaccaaa cccgggccat ggctgaattt ggtttcatct ttggcaacaa
     2701 tatacggtaa gttcccgcac aggcgcggtg ccgggctcaa gcagactggt cgtgtctcca
     2761 gacttttatg caagacagtg agttatcgtt tgtcattccc acgcaccggc tgcgtgacgt
     2821 aggcgaaacc gttgagcagt acgacgagca cttctggcga aatggacact cggttcgaat
     2881 catcgtattc gacgattcga caacgccgaa tcaggagaag tactatccgt tgctcgagca
     2941 gacgcacacc caccaggacg tctattatgt aggacctcgc gaaaaagagc agttcctggg
     3001 ctacctcaat ggcaggttgc gcgaccagcg gttaggggcg ctggtgaaga atctcttccg
     3061 cccaagctat ggtggaaacc gcaacttcac gctgatgtac acactcggcg gactgatggt
     3121 cagttccgac gacgatatgc ggcccttttc gctgatcgaa tacagccggg agtcgctcgg
     3181 agccggcgag gtcagccggg gaagactcca taaagcggat cagaacgggt atacccggaa
     3241 atcgttcgac gtcatggcgg cctttctgga tgtgctgggc cggcccgtta gcgaagtgcc
     3301 ggacaactac gagcgcggcg agctactggt ggacaccgcg atggacctgg aaacaaacgc
     3361 caccaagggc ttggcacacg agaactcgct gattcttgaa cgaggaacag tgcccgcgga
     3421 ctcccgcgtg aagattgccc agacgttccg ctccggcacc aacgacgtgg atgcaatcga
     3481 cttcgttgac atgtttctgg aagacgaagg gcaaacgaat ctcgacacat tgaacgacgt
     3541 atatgtgctg gtcaacttca ggccggtgat cacgaacaag aactggcgaa tggattgcgg
     3601 agtcgcgggc tacgataaca cgtttgggct gcctcccttc ttccccacgc gattgcgctt
     3661 tgaggactac atctaccggc tctgggtaca gcaggacggc gtcgctgccg cccacgtcga
     3721 cgcggcgcag aaccacagca agagcaacta catgcgcaat ccgccggctg cggagatctt
     3781 caacgaggag gtgtcgaatc ttctcaagcg caagatcaag gccagcgtga cgagcctcga
     3841 tgagttaagc atcgctttcg actacgacgg ccatgtcact gtcgaggatg ccagcgagat
     3901 cctcgacaag attactgccc tccacagccg ggcactgctg gcggccgacg cagcacgaaa
     3961 cgcggatcgc gcccaggcac tgcggatttt cgccgcaaac ctggccaagg cattctacgg
     4021 ttttgagccg gatttctttc agcagaacct gacgcgaatc gccgacgatg ttgtcggggt
     4081 gatcaaagcg tcgatccagt tatggccgac actggtcgag atcgcgtact ttcagaaacg
     4141 gcgatccgga ctgccacagg cgcgcgtgga gaaccacagg aaatgacgag gatgcgaatt
     4201 gcgcaggtcg cgccgctgta tgaaagcgtg ccgccgcgcc tctacggagg gacggagcgc
     4261 gtcgtctcct ttctgacgga ggaactggtt cggctgggcc acgaggttac cctctttgcc
     4321 agcggcgatt ccaaaaccat ggcaacactc gtcgctcctt gcgagcgcgc cctctggcgc
     4381 gacccggcct gtcgcgagac gctgccccaa cacgtgcggc tgatggaact cgtctttcgg
     4441 aaggcttcgc aattcgacgt gattcatttc cacaacgatt acctgcactt tccgctcctc
     4501 cggcataacc caacgcccac cgtcaccacg ttgcacggac gtctgcacct gcatgacgtg
     4561 cagcctttgt tccgtgagtt cgcggaagtg ccattggtgt cgatatccga ggaccagcgc
     4621 cgtccgctgc cgggcgccaa ctggcaggcg accgtctatc acggcctgcc gcgagagact
     4681 catacgttcc aggacgagcc gggagagtac ctggtgtttg tcggacgggt ctcgcctgaa
     4741 aagcgactcg accgtgcaat cgagatcgcg aggcgcgcgg gccgaaagct caaggtcgcc
     4801 gcaaaaatcg atgacgagga tcggggctac tatcagcaat cgattgagcc tctgctccag
     4861 gcctccaggt cgttcgtgga attcgttggc gaggtcggtg ggagcgcgaa ggacgagtta
     4921 ctgggcggcg cctgcgctct cctgtttccg attgactggc cggaaccctt tggacttgtc
     4981 atgatcgaag cgctggcttg cggtacgccc gtcatcgcat ggcgcaacgg ttcggtgcct
     5041 gaaatcctgg agaacggaaa gaccgggttt gtcgtggaaa cggtcgccga agcggttgaa
     5101 gccgtccgca aaatcgagtc cctggatcgc ctggtttgtc gtcgagcgtt tgaggaacgc
     5161 ttcgattccg tgcgcatggc acggaactac gtccgggttt acgagcggtt gatccacgaa
     5221 agcccgagcc ggattctcga gtcggcgtct gccacgatgc caataggttc gagggtctga
     5281 gagcatgata caagaatccc ggcgctctga aggaaagcca ggagaggatg cccaccacat
     5341 cctggcggac tcgagcctca ccgatgagtg catccgggta ctgaagcatg gggacacgtt
     5401 tgccctgttc gaccagtacg gcgatatcaa gcccagcaag gcaggcgagc agggcgtcta
     5461 ccatgacggt acgcggttcc tatcgcgctt gattcttgaa ctggacggcc gcagaccctt
     5521 cttcctgagt tcgacggtcc gggacgacaa cgaccagttg accgtcgcac tgacgaatcc
     5581 cgatctctgc cgcgacggtg tggtgtacct tccgctgggc accctgcatc tcgcggtgaa
     5641 gaagttcttg tggcaggggg cgctacacca ggaaatccaa attcagaacc atggagtcca
     5701 gttcgccgac gcaacagtca cgctccaggt cggggcggac ttcgccgata tctacgaggt
     5761 ccgaggtctc aaaagggaag ctcgtggcga ggacctggag cctatcgcca gccttgatcg
     5821 cgtgacgttg cgataccggg gactggacct gaccatccgc cgcaccgtgc tccaatttac
     5881 gccctcaccg aacaggctga ccgccggacg cgccgagttc acagttgcgc tggctccaaa
     5941 ggagacggct tcgttccaac tggttgttgg tttcgagagc gggcccgagg agcggccact
     6001 cgtggctttc gatcaggcgc gagttgaagc aaaggcggat cttggtcgtc acaaagcaca
     6061 gctatgccgc atcgagtctg tgaacggaca attcaacgcc ctggttcaac gggcggcatc
     6121 tgacctgcac atgatgatca ctacgcttcc ttcgggaccc tatccctacg caggcgtccc
     6181 gtggttcaat gcgcctttcg gccgcgatgg cattatcacg gcgctggagt gtctgtggtt
     6241 tcacccgagt ctggcaagag gcgtcctggc ctatctggca tcgacgcagg cgaccgaagt
     6301 catccccggc caggacgcgg agccaggtaa gattctgcac gagacgcgaa atggtgagat
     6361 ggcgaccctt ggagagatgc ccttcggccg ctattacggc accgtggatg cgacgccgct
     6421 tttcgttgtg ttggcgggcg cgtactttga gcgaactggt gacactcggt tcgttgaggg
     6481 gatctggccg aacatcgagg ccgcgttgct ttggatggat cgttacggcg atcgagacgg
     6541 ggacggattc cttgaatacg atcgacagtc gagcgacggg cttttgcacc agggctggaa
     6601 ggactctgac gacgctatct tccatgccga cggctcaccc gctcaagggc cgatcgccgt
     6661 atgtgaagtg cagggctatg cctacgccgc ctggcgggca ggtgcaacca tggcctccgc
     6721 cctcggccgg gcaggtcaag ccgaggattt cgtatctcag gccgtgatgc tgaagacgcg
     6781 atttgaccgg atgttctggt gtgaggaact gtcaacctac ggattggctt tggacggaga
     6841 caagcgcctc tgccgggtgc gcacctcgaa cgccggacaa tgcctcttct ccggaatcgc
     6901 gacgaccgat cgggctgagc gggttgccaa aaccttactg gcgcctgagt cgttttccgg
     6961 ttggggcatt cgctcggtag cagcttctga acaacgatac aacccgatgg gctatcacac
     7021 cggcagcgta tggcctcacg ataacgcatt gatcgcgtac ggcctggcgc gctatggatt
     7081 tgccggggag gccttgcgag tgttcgaggg cttgttcgat gctggcatgt attttgactt
     7141 gcaccggatt cccgagctct tttgcggctt tccacgggat ccgggggagg gtcccgtttt
     7201 gtacccggtc gcctgcgccc cacaggcatg gtcagcgggc gccgtgtttc tcctccttca
     7261 ggcatgtctg ggtttgcggg taggtggatc gaaaccgcag ctatcgtttg ttcgccctac
     7321 gctaccagcg ttcctgggcg agacgagaat tctgaacctg caagtcgcca acgcagaagt
     7381 ggatctctta ctaacccggc atgatcgcga cgtggctgtg aaggtgctgc gccgggaggg
     7441 agacgtagag atcatggttc tgatgtaatc cgatgcgcga tcccgtcgac ctcgtccttt
     7501 ccatgccgga ggaagaacgc cgtacctggt tgtaggcatt ggctcccgat gacgcaaccg
     7561 attctgatcc aggacgctcc ccgaaatgag cgttcatcgc tcctggcggc actggatccg
     7621 tggccatgcg agcgcatcgc cgccggccgt agccaccttg gtgacatctc cgctctcgtg
     7681 atctgttttt ccactgcgaa agtgtttatg ctgagaggtc tgacgtgagc acatacattc
     7741 gaatccgagg atcgcggcat ggcgcgtgag acccgccgga agacgcccga ggagttcctg
     7801 cgcgaagttc aggctgaaga agtcgcggcc aggagg
//
LOCUS       DASRKK010000739         6674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1055342, whole genome shotgun sequence.
ACCESSION   DASRKK010000739 DASRKK010000000
VERSION     DASRKK010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055342"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFC15_17790"
     CDS             <1..555
                     /locus_tag="VFC15_17790"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM12065.1"
                     /translation="GPSDVLHATGGLTNNGTITVKNGSTIDPPPSVNNLGTINIDGTS
                     TFVVGTGTATGPGFIQLSTGTFGEMINSATAFGVVNVAGSASLNGTLDILLQTGYNPA
                     VGTSFTFLLLNPNQLSGTYASIVNDIFNGGTEKWVVNYNDAGGFVSLTAATNNSTVPE
                     PGTFLLLGSGLLSLSYGVRRRLKK"
     gene            696..1070
                     /locus_tag="VFC15_17795"
     CDS             696..1070
                     /locus_tag="VFC15_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12066.1"
                     /translation="MKQRLIALSLLMFVTLILLTGCTPPPTAVTFTTPYQAVLLSNNS
                     VYYGKLAGFGTASPVLTDVFYIVSQTNPETKQVTNVLVKRGKELHGPDRMYLSANSIV
                     FVEPVGADSKVAQLIDEASKKQ"
     gene            complement(1122..2522)
                     /locus_tag="VFC15_17800"
     CDS             complement(1122..2522)
                     /locus_tag="VFC15_17800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZM12067.1"
                     /translation="MTTPQQYEAILIGSGQAANPLSTALAAAGMRTALIERKHVGGTC
                     INEGCTPTKTMVASGRVAYLARRAADYGVHTGPITIDMLKVRQRKRDIVDNFRNGSQS
                     RLEKTANLDLIFGDARFTAPKTIEVCLNDGTRRSLSAKYFFINAGTRAARPRLDGLDD
                     VPTLDNVSIMELDKVPDHLLILGGGYIGLEFGQLFRRFGSRVTIVQSGMQLLTREDPD
                     IAEEIAKILRQDGVNVLLNANATHVKPAADGGIQLEVALPGGPATLIGSHLLVAAGRT
                     PNCDTLNLAAAGLQCDKHGFIQVNDRLESNVSGTYALGDIKGGPAFTHISYDDFRIIR
                     ANLLEKKDASIKDRLVPYTVFIDPQLGRVGMTESEARAQNRSIRVAKMPMTSVARALE
                     VDEARGFMKVVVDAGTNQILGAAVLGLEGGEVMSAIEIAMMGKLPYTALRDGVFAHPT
                     LAESLNNLFTAMDSGK"
     gene            complement(2556..3032)
                     /locus_tag="VFC15_17805"
     CDS             complement(2556..3032)
                     /locus_tag="VFC15_17805"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HZM12068.1"
                     /translation="MSSAFGLLLTFGIGFVAGLRSMTAPAVVAWAAHLGWINLSGTPL
                     AFMGSAWAVALFTLGALGEYVADKFPNTPARTAAIGLGARVVMGLLCGACVGVAAGMS
                     LWIGAIVGAIGAVAGTFGGYQARVGLVRGLQVKDIAVAIPEDLVAIGLGLLLVSRF"
     gene            3377..3826
                     /locus_tag="VFC15_17810"
     CDS             3377..3826
                     /locus_tag="VFC15_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12069.1"
                     /translation="MKRNLIAVLSLVVMSLLLNATGAFAQTYVKANVPFDFKVGSAQL
                     PAGTYEIQADDTIPGTVMIRNHQTSAAAMSIAQREYPRGDTTAKLVFHKVGNQYFLAE
                     IWKGSDAEEMVIPTSRQEKELEKELEKELQASNTHSGGYEEIVVALN"
     gene            complement(3948..5942)
                     /locus_tag="VFC15_17815"
     CDS             complement(3948..5942)
                     /locus_tag="VFC15_17815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM12070.1"
                     /translation="MSPIDLRDGAEGSASRLRNLAATLLSPSLLEAIPDAIVAVSQQG
                     IILQANSQTESMFGYAREELIGQPIEILVPESFRDQHRHHRDQFVEQPKARRMGPGLD
                     LRGRRRDGSEFPVEISLSPLETDDGIVVLSAIRDVSERKRIEAELQRVHHELSERMDR
                     QLWEYQHRLSLIVDSSQDAIIGKNLDGTITHWNKGAEHIYGYEADEVIGRSITLLAPV
                     DRKDEIPRMLESIRNGQRVEHFETVRVAKDGRRLHVAISISPIRDATGEIVGASTIAR
                     NITAQKKAEDQLRQSQKMEAVGRLAGGISHDFNNILGIITMCAELLRTHVASEGLPQE
                     LIGHIRDASKRGAALTRQLVAFSRKQPSQPKLLDLNDRLREVCKLLRPLMGDDVSVQL
                     LPRSELAFVRADPSQLDQIVLNLAVNSRDAMPQGGKFIIETATSEFDEDFVRQHPPMK
                     PGRYVMLAVSDNGAGMTDATRARIFEPFFTTKEMGRGTGLGLATVYGIVQQSGGHIWV
                     YSEPGRGTTFKVYLPCADAEAGMAGEPQQEPEFRRGEGTTILLVEDDPVIRRLAQRML
                     DSQGYAVIEAEDGKSAVAQAQSCPDHINLVLTDVVMHGMSGPELVLRLMESHPETKFL
                     YMSGYTGELVMDNGLGEGIALLEKPFTRAELLSSVQAVLP"
     gene            complement(6026..>6674)
                     /locus_tag="VFC15_17820"
     CDS             complement(6026..>6674)
                     /locus_tag="VFC15_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZM12071.1"
                     /translation="PKPVRVPGLWLGTAERISAIVEMNHPGVWIMGDLADEDRHHGMG
                     IVVEYAGYKGKSQWIPPKPFKWNYAHFGNPGATSAPPDDVFDMLFEKQNAALDGFNQW
                     TINGVAYPMEQMMAAPSFHLHEGKRYRIRMRNASDDIHPIHLHRHSFELTRIAGQPTS
                     GVIKDVVMLGGYQEAEIDFVANNPGLTLFHCHQQLHMDFGFMTLFDYVPTRSELR"
BASE COUNT         1364 a         1961 c         1978 g         1371 t
ORIGIN      
        1 ggtcccagcg acgtgcttca tgctacggga ggcttgacca acaacggaac gattaccgtg
       61 aagaacggct ctaccattga cccgccccca tccgtgaaca atcttgggac catcaacatt
      121 gatggcacct cgacgttcgt agtgggcacg ggcaccgcga cgggaccggg cttcattcaa
      181 ttgtccaccg gaacgtttgg ggagatgatc aactccgcaa ccgcttttgg tgtggtcaac
      241 gtggccgggt cggcttcgct gaatggcacg ctcgatatcc tgctgcagac agggtacaat
      301 ccggcggtcg gcacgtcgtt cacgttcctc ctgctcaacc ccaaccagct ttccggcacc
      361 tacgccagca tcgtgaacga cattttcaat ggcggtacgg agaagtgggt ggttaactac
      421 aacgacgccg gcggctttgt gtcactgacg gcggcaacga ataactctac ggtcccggaa
      481 ccgggtacct tcctgctgct gggcagcgga ttgctcagcc tgtcatacgg cgtacgccgc
      541 cgcttgaaga agtagattta cttcgtctga aacgcggcct gattgaatgc agtcaggccg
      601 tttttttgtc tgcggcgaac caattcagtg cgacaatttg tcgccaacca tttacgatta
      661 ttgcggccag caaaccgggc cccgggagaa ccagcatgaa gcaacgtttg attgcattgt
      721 ccttattgat gtttgtcacc ctcatactgt tgacgggctg cactccgcca cccacggcgg
      781 tcaccttcac cactccctac caggctgttc tgctcagcaa taattctgtg tattacggaa
      841 agctcgcagg cttcggcacg gcgagtcctg tactgaccga cgtgttctac atcgtgagcc
      901 agaccaatcc agagaccaag caggtcacca acgttctggt gaagcgcggc aaagaactgc
      961 atgggcccga ccgcatgtat ctgagcgcca acagcattgt ctttgtggag cctgtgggcg
     1021 ccgattcgaa agtcgcgcaa ctgatcgacg aagccagcaa gaagcagtag ttgacgagcg
     1081 cctcactcaa gctggcttag cttgagtggg atctctcctt ctcacttccc gctgtccatc
     1141 gccgtgaaaa gattgttcag cgattccgcc agagttggat gggcgaagac gccatcgcgc
     1201 aatgcggtgt agggcagttt ccccatcatc gcgatctcga tcgccgacat gacctctcca
     1261 ccttccaagc caaggacggc ggcgccgaga atctggttcg tcccggcatc gacgaccacc
     1321 ttcatgaagc cgcgggcctc atccacctca agcgcgcgcg ccaccgacgt catcggcatc
     1381 ttcgcgacac ggatgctgcg gttctgggcg cgagcttccg actcggtcat gccaacccgt
     1441 cccaactggg gatcgataaa aaccgtgtag ggaaccaggc gatctttgat cgaggcgtct
     1501 ttcttttcga gcagattggc gcggatgatt cgaaagtcgt cgtacgagat atgggtgaac
     1561 gctggaccac ccttgatgtc gccgagcgca taggtgccgc tcacgttgga ttcgaggcgg
     1621 tcgttcacct gaataaatcc gtgcttgtca cattgaagtc ccgcggccgc gagattgagc
     1681 gtatcgcaat ttggcgtgcg tccggcagcc accagaagat gagatccgat gagagtggcc
     1741 ggaccccctg gcagggcgac ctccaactga ataccgccat cggcagcagg tttgacatgg
     1801 gttgcgttgg cattcagcaa aacgttgacg ccatcctggc gcaggatttt ggcgatttcc
     1861 tccgcaatgt ccggatcttc gcgggtgagc agttgcatac ccgattggac gatcgtcacc
     1921 cgactgccga agcgccggaa gagttgtcca aactccaagc cgatgtagcc gcctcccagg
     1981 atcaacaggt ggtcgggaac tttatccagc tccatgatgg agacgttgtc gagcgtggga
     2041 acgtcatcga ggccgtcgag tctggggcgc gcggcgcggg ttccggcgtt gatgaagaaa
     2101 tatttggctg agagcgaacg tcgcgttccg tcgttgaggc aaacttcaat tgtctttgga
     2161 gcggtgaacc tggcatcgcc gaaaatcaag tcgaggttcg cggtcttttc gagacgcgac
     2221 tggctgccgt tgcgaaagtt gtccacgatg tcgcgcttgc gctggcggac tttcagcatg
     2281 tcgatcgtga tcggcccggt gtgcacgcca tagtcggccg cgcgacgggc caggtaagcg
     2341 acccggccgc tggccaccat ggttttggtc ggagtgcatc cttcgttgat gcaggttccg
     2401 cctacgtgtt tgcgctcgat gagagcggtg cgcatcccgg ccgcggccag agctgtcgaa
     2461 agcggattgg ccgcctggcc ggacccgatg aggatggctt cgtactgctg cggagtggtc
     2521 ataaggcgat cctgcgaagc gcgatttgca gagtttcaga accgtgaaac taagagcagt
     2581 cccagtccta tggcaaccag gtcttcggga atggcaacgg caatgtcctt aacctgcaag
     2641 ccacggacca gcccgacgcg cgcctggtag ccgccaaagg tcccggcaac cgcgccaatc
     2701 gcgcccacga tggcgccgat ccaaagtgac atgccagccg caaccccgac gcaggcgccg
     2761 cagagcaggc ccatgacaac tcgcgcaccc agcccgatgg cggcagtacg tgcgggggtg
     2821 ttagggaatt tgtcggcgac gtattcgccc aaggcaccca gcgtgaagag ggcgacggcc
     2881 catgcggatc ccatgaaagc cagtggagta ccggaaagat tgatccatcc caggtgcgcc
     2941 gcccaggcta cgacggcagg cgcagtcatg gagcgtaatc cagctacgaa cccaattcca
     3001 aacgtcagca acagtccgaa tgcagaactc atcgaacgtc ctccatggag acgaagattt
     3061 agttgagggt ccggattacc aaggaatgga tgacggcgag gcgggagaga ttcagttttg
     3121 atcgagaacc gaagagatac tcgcgggtat ccaatcggct accgatttgc tgcattccga
     3181 cactgccttc aaaggccgct gaaacctatg ttgttgcaaa tactgtgaaa cgactaggca
     3241 accaggcccg gctgtgcttt ggcacgctgc ttgctctatg taaacgcgtc acagcaagct
     3301 gccagcgatg gcgaagcgaa gctcaaagac aacttcagtt tgggttccac tgcaaggagc
     3361 ccccaaggag aatgttatga aacgcaacct gatcgcggtt ctgtcgctgg tggtgatgtc
     3421 actgctcctc aatgccacgg gcgcctttgc tcaaacttat gtgaaggcca atgtgccttt
     3481 tgatttcaag gtcggatccg cgcaactgcc ggctggcacc tacgagatcc aggccgatga
     3541 caccatcccg ggcacggtca tgatccgcaa ccaccagacc agcgcagccg ctatgtcgat
     3601 agcccagcgc gaatatcccc gcggcgacac gaccgccaag ctggtgttcc acaaagtcgg
     3661 gaaccagtac ttcctggcgg agatctggaa gggctccgac gccgaagaga tggtcattcc
     3721 cacctcccgg caggagaagg aactggagaa ggaactggag aaggaactgc aggcttccaa
     3781 cacgcactcg ggtggatacg aagagattgt agtcgcgctg aactaacatc ctcctctcac
     3841 cgaacccaaa cgaggaagcc ggagccaacg cgctccggct ttttcatggt gcggcgatcc
     3901 tccccactta ctcgaacggc gcgagtcggg cagggagccc ggtttcgtta tggcaatacc
     3961 gcttgcacgg agctcagcaa ttcggcccgc gtgaatggct tctccaacag cgcgattccc
     4021 tcgcccaatc cgttgtccat cacgagttcg cccgtgtaac ccgacatgta gaggaacttg
     4081 gtttcgggat gcgattccat gaggcgcaaa acgagttccg gcccactcat gccatgcatg
     4141 acgacgtcgg tcagaaccag attgatgtgg tccgggcatg actgggcttg cgccaccgcc
     4201 gacttgccgt cctcggcttc gatgacggca tagccttggg agtccagcat tcgctgggcc
     4261 agccggcgga tcacgggatc gtcttcgacc agcagaatgg tggtgccctc gccgcgccgg
     4321 aattccggtt cttgttgcgg ttcgccagcc attccagctt ccgcgtcggc gcagggcagg
     4381 tacaccttga aggtggttcc gcgacccggt tcactataga cccagatgtg tccgccactt
     4441 tgctgcacga ttccatagac cgtagccaga cccagtccgg tgcctctacc catctccttg
     4501 gtagtgaaga aaggctcgaa gatacgggcg cgggtcgcgt cggtcatgcc ggctccgtta
     4561 tcgctgaccg ccagcatcac gtagcggccg ggcttcatgg gtggatgctg gcggacgaag
     4621 tcttcatcaa actccgaggt cgcggtttcg atgatgaact tgccgccttg aggcatcgcg
     4681 tcacgggaat tcaccgccag gttcaacacg atttgatcaa gctggctggg atcggcgcgg
     4741 acgaacgcca actccgatcg gggcagaagc tgaacgctga catcgtctcc catgagcgga
     4801 cgcagcagct tgcagacttc ccgcaagcgg tcgttgagat cgagcagctt gggttgcgag
     4861 ggttgcttgc ggctgaaggc gaccagttga cgggtgagag cggcgccgcg tttggacgca
     4921 tcgcgaatgt gaccgatcag ctcctgggga agtccttcgc tggcgacgtg ggtccgaagc
     4981 aattcggcgc acatcgtgat gatgcccaga atattgttga agtcgtgcga gatgccgccc
     5041 gcaagccgtc ccacggcttc catcttttgc gactgccgaa gctggtcctc ggctttcttc
     5101 tgcgccgtga tgttgcgggc gatggtggaa gctccaacga tctcgccggt ggcgtcgcga
     5161 atgggagaaa tcgagatcgc gacatgcagg cgccgtccgt ctttggccac ccgcacggtt
     5221 tcaaaatgct cgacccgctg gccgttgcgg atgctctcca gcatccttgg aatctcatcc
     5281 ttgcgatcga ccggcgccag cagcgtgatc gacctgccga tgacctcgtc ggcctcgtag
     5341 ccgtaaatgt gctccgctcc cttgttccag tgggtgatgg tgccatccag attcttgcca
     5401 atgatggcgt cctgggaaga atcgacaatc agcgagagcc ggtgctgata ttcccagagt
     5461 tgccggtcca tgcgctcgct cagttcatgg tgcacccgtt gcagctccgc ttcgatgcgt
     5521 ttgcgctcgc tgacgtcgcg gatagcgctg agcaccacga tgccatcgtc cgtctccaac
     5581 ggactcaggc tgatctcgac gggaaactcc gagccgtcac gccgccggcc gcgaagatcc
     5641 aagccgggtc ccatgcggcg tgccttgggt tgttccacga actggtcgcg atggtgacga
     5701 tgctggtcgc ggaagctttc gggtacgagg atttcgattg gctgtccgat caactcttcg
     5761 cgtgcgtatc cgaacatgga ttcggtctgc gaattggcct gaaggatgat gccttgctga
     5821 ctgaccgcca caatcgcgtc gggaatggcc tcgagcaggc ttggcgagag gagcgtcgcg
     5881 gccaaattgc gcagccgact ggcactgcct tccgctccgt cgcgtagatc aatcggactc
     5941 atatccccac ccgggccaag ctcttgggct taactatagt ccaagttgta gagcgaggaa
     6001 tacgaccctg ggatttgacg ccgaactagc gaagctcgga ccgggtgggc acgtaatcga
     6061 acaaggtcat gaagccgaag tccatgtgta gttgctgatg gcagtgaaac aacgtgagac
     6121 cggggttgtt ggcgacgaaa tcaatctcgg cttcctggta tccgcccagc atcactacgt
     6181 ctttgatcac ccctgaagta ggctgcccgg cgattcgcgt cagctcgaag ctgtggcgat
     6241 gcagatgaat cgggtggatg tcgtcgctgg cattacgcat tcgaatgcgg tagcgcttgc
     6301 cctcatgaag atgaaacgaa ggcgccgcca tcatctgctc catcgggtag gcgacgccgt
     6361 tgatggtcca ctgattgaat ccatcgagcg cggcgttttg tttttcgaag agcatgtcga
     6421 atacatcgtc cggcggcgcg gatgtcgcgc cgggattgcc gaagtgcgca tagttccact
     6481 tgaagggctt cggtggaatc cactgtgact tgcctttata accggcgtac tcgaccacga
     6541 tgcccatgcc gtgatgacgg tcctcgtccg ccaggtctcc catgatccag accccaggat
     6601 ggttcatctc gacgatagcg gagatgcgct ccgccgtgcc cagccacaga ccgggcacgc
     6661 gaaccggttt cggc
//
LOCUS       DASRKK010000740         5066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_664573, whole genome shotgun sequence.
ACCESSION   DASRKK010000740 DASRKK010000000
VERSION     DASRKK010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664573"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            793..2262
                     /locus_tag="VFC15_17825"
     CDS             793..2262
                     /locus_tag="VFC15_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12072.1"
                     /translation="MNKTNVVWLLFLVLTLTLLAGCGSSSSPPTPAPLSAGNLNLIFV
                     VSEDLAYHATGDVNPGTANLTNQGFQRSLLMATFLQQQVLGTQNVTGIYALEPMTHLQ
                     TANNYPDIVALETIQQFAMLNQTSPSYLGSGPVTANSYPIFASYSLEAALPDDVAPPA
                     YPCAACQGLDFRDQHGDNEALVTGIVKANVPGFYVFSAPWETTRSLLTNINRLKGYNL
                     SLPPSYISPNYIYAISIAPSGNASLGTYNSDLIPPSTYPVLPPPPLVSTPCNTPQTQA
                     FFSITVTGGINDAVIPAGTNTNETVYFVRHADAHPTTWWDDGNYIGKGQWRALDLPNA
                     LRDKIHPTQVYAIDPSVGNPGVDGSVASSYVRPSLTVEPYAIANNLPFNLAAIVPVFS
                     QNPPQPSTAASLYFFNGGTFSNQTILVGWEHDHIPPTVNALLTSYNAGPLPSSDTWPD
                     TDYDTIWTVKLDAVGNLTVNNGMCEGISSTALPDTPPQF"
     gene            complement(3069..4124)
                     /locus_tag="VFC15_17830"
     CDS             complement(3069..4124)
                     /locus_tag="VFC15_17830"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZM12073.1"
                     /translation="MPGVFISYRREDSAAYAGRLFDILSTEFGRENTFMDLDTIEGGD
                     NFSVVIERKLDVSDALLAVIGPHWLTVTELNGGRRLDNESDLVRVEIAKALERGIRVI
                     PVLVGGATLPRPVDLPTKLQALCERQAMEVRDSHFHADVKDLTDVLHRTLHGTGFVPK
                     AANLKRFIPAFLVGLAVVIGIFWLLVQRRMVQRQNVERAASVAAATKQAAQNSGSAPP
                     PAQTSAGGTSNSVKPLANVAGQWEATVKYDWGDTYHEWFDFEVDGQELSGSAGFLGNR
                     KGQGRAIWDGKIAGDRISFMTKTLSSLGGDDKTYEDKHYYKGTVKGDEIVFTMVTDSS
                     TESHTPIHFTANRVKNK"
     gene            complement(4688..>5066)
                     /locus_tag="VFC15_17835"
     CDS             complement(4688..>5066)
                     /locus_tag="VFC15_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12074.1"
                     /translation="NASFIDQGYFFNAGEWSFPDAPLRGVFGRNDVHAGITGWGSFEP
                     WLSRIESFPESSLWPLVDQIPPELYGSDVDELDRLLTQLLERRSGVRELILSFKNSSR
                     NPFPNWREVYSVAAATSLRPRGQ"
BASE COUNT         1092 a         1498 c         1414 g         1062 t
ORIGIN      
        1 tggcgcccac cttccacgag gccgaggcat cggcgaagca taatgcctcg atctggatga
       61 cgaaggtcga agtgccccac gctggattgc cctagcggcg tagcgctatc gctgagggca
      121 tgtcgcaacg gcaattggcc atgacacgta tttgaatcgg tcagccccgc caagacgcga
      181 gttcccttga agtcgggttg gaggaagttt cacttggccg aatagtcgcc tgcgcgtgga
      241 agtgtgatgg atctgctctt tagatgcgcc acatccgcgc gtagggcgtt ttacgtcaag
      301 ttgatcgggg acgaaggtaa acgctgcgcg gcgcatcgaa ggctgttcgc gcgccgcttc
      361 ccaaagcggg cactttcaat cggcgacgcg ccgtccgcga gcgcgtgata aacaaattgg
      421 ctccgattgc gacgctcgca cttccgtctc acaaagggtc gttattggga cacgattggg
      481 gtgatgtaac caaccccgct aagtagttga ttcatcgcga tcggcgccac cgctttcagg
      541 gcacgttttt gggacggcat cgacggaagg tgagactgat cgtgacggaa gttcttccag
      601 gtacacagcc agccccgcac ttcatccttg taagtctcgt cgggtaaaga tcaaccagca
      661 aagtgccgat atcaatcagt tcaagtgtgt ccgctgaagc ggatatggaa tattgcacgg
      721 attgtggtcg cctgattcca gatgttgctc actttcgcag ggagtgtggg gctcgccagg
      781 agatacaaga tcatgaacaa aactaacgtt gtttggctgc tgttcttggt tcttaccttg
      841 acgctgctcg caggatgcgg cagctcttct agcccgccaa ctcccgctcc actgagcgca
      901 ggcaacctca acctgatctt cgtggtaagc gaggatttgg cttaccacgc gaccggggac
      961 gtgaatcccg gcacggcaaa tctcactaac cagggcttcc aacggtcgct cttgatggcc
     1021 acgttcctgc aacagcaagt actgggaacg cagaatgtaa ccggcattta cgcgctcgaa
     1081 ccgatgaccc atttgcaaac tgcaaacaat tatcccgaca ttgtcgcgct ggagacgatt
     1141 cagcagttcg ccatgctgaa tcagaccagc ccgtcgtacc tgggcagcgg gccggttacc
     1201 gcgaacagct atcctatctt tgcatcgtat tcgttagagg ccgcattacc ggacgacgtt
     1261 gcgccgccgg cgtatccctg tgcggcttgc cagggtctcg acttccgcga ccagcacggc
     1321 gacaacgaag ccctggtaac cggtatcgtg aaggcaaacg tgccggggtt ttatgtcttc
     1381 tcggcgccgt gggagaccac ccgcagcctg ttgacaaata tcaacaggct gaaaggctac
     1441 aacctgagcc ttccgccgag ctatataagt cccaactata tctatgcgat ttcgatcgcg
     1501 ccgtcgggca acgccagcct gggtacctac aacagcgacc tgattccgcc ttccacctac
     1561 cctgtgctgc ccccgccgcc gctggtcagc actccctgca acacgccgca gacgcaggcg
     1621 tttttcagca taacagtgac cggcggtatt aatgacgccg taatcccagc gggtaccaac
     1681 accaacgaaa cggtttactt tgtccgccac gcggacgcgc atcccaccac ctggtgggac
     1741 gacggcaact atattggcaa ggggcagtgg cgcgccctgg accttcccaa cgcattacgc
     1801 gacaagatcc atcccacgca ggtttacgcc atcgatccct ctgtcggcaa cccgggcgtt
     1861 gacggcagcg ttgcatcgtc ttacgtcagg ccttcgttga ccgtagaacc gtacgccatc
     1921 gccaacaact tgcccttcaa tctggccgcc atcgtccccg tcttctccca gaatccgccc
     1981 cagccatcca ccgcagccag tctttatttc tttaacgggg gcacgttctc taaccaaacg
     2041 atactggtgg gttgggagca cgatcacatc ccaccgaccg taaatgcgct gctcaccagc
     2101 tataacgctg gcccactgcc atcttccgac acgtggccgg acaccgacta tgacaccatc
     2161 tggacggtga agcttgacgc cgtaggcaac ctgacggtga acaacgggat gtgcgaaggc
     2221 attagttcca cggcgctgcc ggacacgcct cctcaattct gaggctcgct caaatcgaag
     2281 ggaacggacg tgcagataag tagatgctta ttgccaggat cgccaagtcg cgagaccgac
     2341 tcgaaacggt gacaccgctc atgtcggcgc tgaatctggg tgcggttcac agacatcgat
     2401 ggttcctcag acaagcagag taatagtgcg ccagcgggtc acacggtgag ttcacatccc
     2461 ctgcgctcac cgcatagcag gaatcgctgt cgaggcccgg ccacagcttg cgatccacat
     2521 actcgtaggc acggctgatg ttctctgtct tgtggttgcc cacttctggg tgagtcccac
     2581 gaccgagcgc agcgtcatgg cgttgatgga gaggttgcgt atccgagcaa agcaaaaagg
     2641 ttgcaaacgc gcagcacacc tatgatgaag cgctggccaa ggaactggcc tcgactgctg
     2701 accagattgc cgagaccgag caggagttgg ctttagcgcg gcagggctcg gaagtaatcc
     2761 agtttgacgt atagtcggca aaccgccgac tgagagcgga cgacacgccg tacaagaaag
     2821 gtttgggcgt tttacgtcaa gttggcggtg ccaatgcggt tgacggatgc tgggccgcca
     2881 gatcagcgcc ggggcggctg aatcatggaa agcgaagaac cccggcggac gtgttggtgt
     2941 tggccattgc gaatggaatc gatcgagagt tcggctaccg gcctccgccg atgttgcagc
     3001 acgccgggta aaagggccga tatttcattt tcaggtgaat ctccacttgt cactattaac
     3061 gcgccagttc acttattctt gacgcggttt gcggtgaaat gaatcggtgt gtgggactca
     3121 gtgctgctgt cggtgaccat ggtgaaaacg atctcgtcac ctttgaccgt gcccttgtag
     3181 tagtgcttgt cttcgtaagt cttgtcgtcg cctcctagac tcgagagggt cttcgtcata
     3241 aaactgatcc ggtcgcccgc aatcttgccg tcccatatcg cacggccctg gcccttacga
     3301 tttccgagaa aacctgcgga accggacagc tcctggccat cgacttcaaa atcgaaccat
     3361 tcgtggtagg tgtcgcccca atcgtatttc acggttgctt cccattgccc ggcaacgttg
     3421 gcgagaggct tgacggagtt actcgttccg cccgccgacg tctgggcggg cggcggtgcc
     3481 gatcccgagt tctgcgcagc ttgtttcgtg gctgcagcga cggaggccgc tcgctcgacg
     3541 ttttgccgct gaaccatccg gcgctggacc agcagccaga agatgccgat caccacggct
     3601 aaaccgacca gaaacgcagg gatgaatcgc tttaggttcg ccgccttcgg gacaaagccg
     3661 gtaccgtgaa gtgtgcgatg aagtacgtcg gtcagatctt tcacatcggc atggaaatgc
     3721 gagtcgcgca cttccatcgc ctgccgctca cacaacgcct gcagcttcgt gggcaagtcg
     3781 actggacgcg gcaaggtcgc tccgcctacc aggacgggaa tcacccgaat gccgcgctcc
     3841 agggccttcg cgatttcaac ccgcaccaaa tcgctctcgt tatcgagcct tcgcccgcca
     3901 ttcagctcag tcactgttag ccaatggggg ccgatcacag ccagaagagc atccgacaca
     3961 tcgagcttcc gctcgatgac cacgctaaag ttgtcgccgc cctcaatcgt gtcgagatcc
     4021 atgaacgtat tttcccggcc gaactcggtg ctaagaatgt cgaacaaccg cccggcatag
     4081 gcggcactgt cttcgcggcg ataactaatg aacactccag gcatggttgt atggcctgtt
     4141 taggcagaat agtggaattg tacttgggtg tcgccaagaa caagagcccg ttctttccgt
     4201 tcggatgagg agtatgccca ggttggccca acgcaaaggc gaacccgagc gcctggtgaa
     4261 tggctagttc gacagatgga aagcgaaaag attactgccc gacggcgtga aaactatgcc
     4321 attggcgacc gtaggggaag aaaaggtgga ctcgttaccc actcttgctt tccacagttg
     4381 tgcgccagtg cttgcattca ggctataccc attatttccg cccaggacgc agacgactcc
     4441 tttggctgaa gcggggcgac gatctgctta cgactgcgct tcttgcgtgc catctgccag
     4501 atcctctcgt accagattct ctctcattcc acctggtaca aaattcgccg gtcttgtcac
     4561 ggagtcgagc cggcataaat gaggccaatg tccgtgttgt cggcgactcg tacactggcc
     4621 caaacgaggc tgtgggagcc gtgaattaga tttccggccg tgtcgtcggg gacacggata
     4681 tgaccagtca ttgtcctctt ggacgaagtg aagtcgcagc ggcgacagaa tacacctcgc
     4741 gccagttcgg gaagggattc cgagatgaat tcttgaagga cagaatcaac tcgcgcaccc
     4801 cggagcggcg ctcaagcaat tgggtcaaca acctatcgag ttcgtccacg tcagagccgt
     4861 acaactctgg aggaatctga tcgacgagcg gccagagcga actctccgga aagctctcga
     4921 tcctgctcag ccacggctcg aagctccccc accctgtgat gccggcgtgc acgtcgttgc
     4981 gtccgaagac gccgcgcaac ggcgcgtcgg gaaaactcca ctcccctgcg ttgaaaaagt
     5041 atccctgatc aatgaagctg gcgttg
//
LOCUS       DASRKK010000741         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_469203, whole genome shotgun sequence.
ACCESSION   DASRKK010000741 DASRKK010000000
VERSION     DASRKK010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469203"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFC15_17840"
     CDS             <1..1116
                     /locus_tag="VFC15_17840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM12075.1"
                     /translation="PAGSSCAWGLRDSGLDILILDRSAFPRHKLCGGWITPLVLDELN
                     IDPRHYAPDRTLQPISGFRLSAIGGPQVDVRCDGVVSYGIRRCEFDEYLLRRSGARLR
                     EGEPLISIERSDRGWLVNGTIRARILIGAGGHFCPVSRYLGNKGAPAPVLAQEIEFEM
                     DPAQASTSKIAGDLPELFFCRDMRGYGWIFRKDNFLNVGLGRTDSHEISRHVKDFVRY
                     LKKTRGVEVPSNGISGHAYGLFGHSHRNILDDGVVLIGDAAGLAYPESGEGIRPAVES
                     GLIAAHTILVANGDYRTRQLALYRDLLTSRLQRERSRIDTLSQLLPHTLREILGRQLL
                     RNNSFCRNVVIDRWFLRVAEQRLAFEPRPVQQELTAT"
     gene            1209..1787
                     /locus_tag="VFC15_17845"
     CDS             1209..1787
                     /locus_tag="VFC15_17845"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZM12076.1"
                     /translation="MGEQTGNWTTTIRQHPERAVPQRAQEFLAQGQVAHVGFVEDGRP
                     YVIPMLYQYSPERPDRLYLHSGFASRTLLRLAEGIPVCVTVTMLDGLVYSRDAKYHSA
                     NYRSVMCFGRGHIVEDEQVKRAMFEAMTTRYFPGRTAGRDYTPAPSSHLTTTAVVEVV
                     IEEISAKMREGGPKGPQDAKDAVPGTCGVVDV"
     gene            complement(1788..3230)
                     /locus_tag="VFC15_17850"
     CDS             complement(1788..3230)
                     /locus_tag="VFC15_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZM12077.1"
                     /translation="MKLFSRRYFSIAVLLVMVGLNPAASLGQTEKPAASGAAQRNDPL
                     HAFSQSIQALTATVTKSVVQIVATGYGFSSGKESSQTALFEPQEAIGSGVILTADGYI
                     VTNAHVVQGARKIRVRLPGLETPGPDGSKPHGPVSAKLVGLDKQSDLAVLKIDATDLP
                     ALPLADSDELKQGQVVFAFGSPQGLENSVTMGVVSATARQVSDENPTTFIQTDAPINP
                     GNSGGPLVDVDGRVVGIDTFILSESGGSEGLGFAIPSNVVRNIYEQLKTEGHVHRGQI
                     GVYLRTITPDLAEGLKLPRDHGVLLEDVSPGSSAEKAGLKAGEVVTNIGGKPVGDTKE
                     FATQLFAYKIGQKAPLGVVRDGKVVTIDVTLIERPDDPQRFADMVTGPGNVVNRLGIV
                     GVTINDELRKMLPDLRIPSGVIVAARTPASSLLGEGPEPGDVIHAVNTSPVTDIAGLK
                     QVLRAIQSGDPIVLQIEREGGLSYLAMESE"
     gene            complement(3416..4660)
                     /gene="hpnI"
                     /locus_tag="VFC15_17855"
     CDS             complement(3416..4660)
                     /gene="hpnI"
                     /locus_tag="VFC15_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZM12078.1"
                     /translation="MMLFLRLLLLVAAAGLVTSTGYLVLVMIASARFRRNKRSRTDAA
                     VFPPVTLLKPVCGMEPGLEANLTSFFEQQYPSFEIIFGARQSDDPALDVVRRISRKYP
                     SAAVKIVATGEPLRPNAKVCSLVKMYAHAAHDYLIISDSDVRVEPNYIAEVVRPLLEP
                     GNGLVTCLYHGVATGGLWSKLEALGMSVEMTSGAIVANLIEGMKFALGPTMAIRRDAL
                     DAVGGFELLADYCADDYVLGREVHESGRQVVMSEHVIDHVVINRSFACSMRHQIRWMK
                     STRFSRRAGHAGTVLIFAMPFGLLGLLAGGALGHWMLGIGLFAAAYLNRVLLAVVSGW
                     GVVRDRQALRLAWLYPLRDLMGFAFWCASYSGSMIEWRSDWYRLEAGGRMVLTQPSAA
                     TLARSLADTGAASPSEVADRYS"
BASE COUNT          986 a         1609 c         1357 g          933 t
ORIGIN      
        1 cccgccgggt cctcctgcgc ctggggacta cgtgactccg gactcgacat actcatcctc
       61 gaccgcagcg ctttcccgcg ccacaaactc tgcggcggat ggatcacgcc gctggtgttg
      121 gacgaattga acatcgatcc ccggcactac gcacctgacc gaacgttgca gccgatctcc
      181 ggatttcgcc tcagcgccat cggaggcccg caagtcgacg tccgctgcga tggagtcgtc
      241 agctacggca ttcgccgctg cgagttcgac gaatacctgc tgcggcgaag tggagcgcgg
      301 cttcgcgaag gcgaacccct catctcgatc gagcgctctg accgcggttg gctggtgaac
      361 ggcactatcc gcgcccgaat cctcatcggc gccggaggac atttctgtcc cgtctcgcgt
      421 tatctcggca ataaaggtgc ccccgccccg gtgttggcgc aggagatcga gtttgagatg
      481 gatccggcgc aagcgagcac ctcgaaaatc gccggcgatc ttcccgaact tttcttttgc
      541 cgcgacatgc gcggctacgg ctggatcttt cgcaaggaca acttcctcaa cgtcggactc
      601 gggcgcaccg attcccacga gatctcgcgc catgtgaaag atttcgttcg ctatttgaag
      661 aagacccgcg gggtcgaagt gcccagcaac ggtatctccg gccacgccta cggactgttc
      721 ggccactcgc atcgcaacat cctcgacgac ggcgtggtgt tgatcggcga tgccgcggga
      781 ctcgcgtatc cggagagtgg cgaaggcatc cgccccgccg tcgaatcggg tctgatcgct
      841 gctcacacga ttctcgtcgc gaatggcgat taccgcacga ggcagctggc gctgtaccgc
      901 gatctgttga cgtcccgatt gcagcgtgag cgtagccgca tcgacacact gtcgcagctc
      961 cttccccaca ccttgcgcga aatattgggg cggcaattgc tgcgcaacaa ttccttctgc
     1021 cgcaatgtgg tcatcgaccg ctggtttctg cgcgtcgccg agcagcgctt ggcgtttgag
     1081 ccgcggccag tgcagcagga actcactgcc acctaaacac aaggtgtccc cgcgacgaat
     1141 tgcagcagtt atgctgcact tcccatctgc aacaatttca gccatgctag agacactgga
     1201 gaatcttcat gggtgaacaa accggcaact ggacaaccac catccgccag catcctgaac
     1261 gcgccgtacc gcaacgcgcg caggaatttc tcgcgcaggg ccaggtcgcc cacgtcggat
     1321 tcgtggagga cggtcgtccc tacgttatcc ccatgctcta tcagtattcg cccgagcggc
     1381 ccgaccggct ttacctgcac agcggcttcg ccagccgcac gttgctgcga ttggcggaag
     1441 gcattccggt atgcgtcacg gtaacaatgc ttgacggcct ggtgtactca cgcgacgcta
     1501 aatatcactc cgcgaattac cgcagcgtga tgtgttttgg tcgtggccac atcgtagaag
     1561 atgagcaggt gaagcgtgcg atgtttgagg ccatgaccac gcgctacttt cccggacgca
     1621 ccgctggacg cgattacacg cccgcaccgt cttcccacct gacgacaact gccgtggtcg
     1681 aagtagtgat cgaagagatc agcgccaaga tgcgcgaagg cggccccaaa ggcccgcagg
     1741 acgcgaaaga cgcagttccc ggcacctgcg gcgtggtgga tgtttagcta ctccgactcc
     1801 atcgccaggt agctcagtcc gccttcgcgc tcaatctgca gcacgatggg atcgccggac
     1861 tggatcgctc gcagcacctg cttcaggccg gcaatgtccg tgaccggact ggtgttgacc
     1921 gcatggatca cgtcgccggg ttccgggcct tcgcccagca acgagctcgc cggggtgcgc
     1981 gcggccacaa taactccgga aggaattcgc aggtccggta acatttttct caattcatca
     2041 ttgatggtga caccgacgat gcccagccgg ttcaccacgt tgccgggccc ggtcaccatg
     2101 tccgcaaagc gctgcggatc gtcgggccgc tcaatcaggg tcacatcaat cgtgaccacc
     2161 ttgccatcgc gcaccacgcc gaggggcgct ttctgcccga tcttgtaggc gaacaactgg
     2221 gtcgcgaact ccttcgtatc ccccaccggc ttgccgccga tgttggtcac cacctcgccc
     2281 gccttcagcc ccgctttctc ggccgatgac ccaggcgaaa catcttccag cagcacaccg
     2341 tggtcacgcg gcagcttcaa tccctcggcg aggtcgggtg tgatggtgcg gaggtacaca
     2401 ccaatctgtc ctcgatgtac gtgcccttcg gtcttaagct gttcgtagat gttgcgcacc
     2461 acgttgctgg gaatggcaaa gcccagccct tcgctgcctc cggattcgga gaggatgaag
     2521 gtgtcgatgc ctacgacccg gccatcaacg tccaccagcg gacctccgct attgccagga
     2581 ttgatgggcg catcggtctg aataaaagtg gtcggattct cgtcgctgac ttgccgcgcc
     2641 gttgcgctga ccactcccat ggtgacggaa ttttccagcc cctgtgggct gccaaaggcg
     2701 aatactactt gtccttgctt gagttcgtcg gagtcagcca gcggtaacgc aggtaaatcc
     2761 gtggcgtcga tcttgagcac agcaagatcg gactgcttgt cgagtcccac cagtttggca
     2821 ctcaccgggc catgtggctt cgagccatcc ggtccgggcg tttccagtcc cggcagccgc
     2881 acccgaatct tgcgcgcgcc ctgcaccacg tgcgcgttgg tcacgatgta cccgtccgca
     2941 gtcagaatca cacccgaccc gatggcctcc tgcggctcga acaacgctgt ttgggaggat
     3001 tctttcccgg agctgaagcc atagcctgtg gcaacgattt gcaccaccga tttggtcacc
     3061 gtcgcggtca gggcctgaat cgattggctg aaagcgtgca acggatcgtt gcgctgtgct
     3121 gcgccagacg cggcaggctt ttcggtctgg cccaaactgg ccgcgggatt caacccgacc
     3181 atgacaagca aaactgcaat ggaaaaataa cgtcgagaaa atagcttcat tgttaaaggg
     3241 ttctcactgg gttgccactt agacattaca gcagaatgat gggccaatca ctattggcag
     3301 acacccattg gatgatcgga agcgcagcca cggcgcagga ttccgggctg attacaatct
     3361 cacactcatt gttccagttt ggcggtgccg aagttccaca aaccccgtga gcgcttcacg
     3421 aataacgatc agccacctct gacggtgagg ccgctccggt gtccgccagg gatcgcgcga
     3481 gggtggccgc gctgggctgc gtcagcacca ttcgcccgcc cgcttccagc cggtaccagt
     3541 cgctgcgcca ttcgatcatg cttcccgaat agctggcaca ccagaaggca aagcccatca
     3601 agtcgcgcag tggatagagc cacgccagac gcagcgcctg gcgatcgcgc accacgcccc
     3661 acccggacac aaccgccagc agcacccggt tgagatacgc cgcggcgaac aggccgattc
     3721 ccaacatcca gtgacccagc gcgccgcccg ccagcagtcc cagcaaaccg aacggcatgg
     3781 cgaaaatcag caccgttccc gcatgtccag cgcgccggga gaaacgcgtg ctcttcatcc
     3841 agcgaatctg gtgccgcatc gaacacgcga agctgcgatt gatcaccacg tgatcgatga
     3901 cgtgctccga catcaccacc tgccggccgc tctcgtgaac ttcgcggccc agcacgtagt
     3961 cgtcagcgca gtagtcggcc agcagttcga atccgccaac cgcgtccagt gcgtcgcgcc
     4021 gtatcgccat cgtagggcca agagcaaact tcatgccctc aatcaaattg gccacgatcg
     4081 cgcccgacgt catctccacg ctcatgccca gcgcttccag cttcgaccac aatcccccgg
     4141 tggccacgcc gtgatacaag cacgtcacca gcccattgcc gggctcgagc agcggccgca
     4201 ccacctcggc aatgtagttg ggctcaaccc gcacatcgct atcgctgatg atcaggtaat
     4261 cgtgtgcagc gtgggcgtac atcttcacca gcgaacacac ttttgcgttg ggacgcagcg
     4321 gctcgcccgt ggcaacaatc ttcaccgccg ccgatggata cttgcggctg atccgccgca
     4381 ccacatccag cgcgggatcg tcggactggc gcgctccaaa gataatctcg aaactgggat
     4441 actgttgctc aaagaagctg gtcaggttgg cttccaaccc gggctccatg ccacagaccg
     4501 gcttcagcaa ggtgacgggc gggaagacag cggcatctgt ccggctccgc ttgttacggc
     4561 gaaaccgggc gctggcaatc atcaccagga ccaggtaccc ggtactcgtc accaaccctg
     4621 ccgctgcgac caacagcagc agtcgaagaa acaacatcat tccaatcccc cgaagcgcgc
     4681 acttatcagt tgggctgaag ccacgggcgc atatacggcc aacggaacaa gtaattctgc
     4741 cgcgccacag cccatctcgt tcgtcgcacc aggacgaatt cttcgtccgt gccagaaccc
     4801 aaccctgtag ctatacttat actgacatta taagggagtc ctcatgtctc tcactcagca
     4861 agactcagct tccgtcagcc aggaa
//
LOCUS       DASRKK010000742         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1056460, whole genome shotgun sequence.
ACCESSION   DASRKK010000742 DASRKK010000000
VERSION     DASRKK010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056460"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFC15_17860"
     CDS             <1..862
                     /locus_tag="VFC15_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZM12079.1"
                     /translation="AKGLAILVTTGICAAAQPPSSFQDKSSGSASQVSPADLSNVGIE
                     QRLDQQVPLDLQFKNETGKTVQLGDYFKSGRPVLLNLVYYTCPMLCGEELAGEASALG
                     VLRFTPGNEYEVVSVSFNPDETPKDASEKKQVYIARMNEHLEHKTNGEGWHFLTGQQS
                     EIKQLADAVGFHYRRDPRTGQFIHAAGIMIVTPAGKIAQYYYGVEFSPKDIRLGLIEA
                     SRDKIGTLVDQVTLYCYHYDPKTGRYGAVVTNMMRLAGAATMLILGGFLVVMFKRESN
                     GGKNGTGRAR"
     gene            865..1851
                     /gene="coxB"
                     /locus_tag="VFC15_17865"
     CDS             865..1851
                     /gene="coxB"
                     /locus_tag="VFC15_17865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZM12080.1"
                     /translation="MWKNLPLFPERASALAWQVDGLYFLLIAVSAFFTLLIFALIFVF
                     AVKYRRSAHPHAVQIEGSLPLELAWTLIPLGICMIFFAWGSLIYFQEARPPKGAMEVY
                     AVAKQWMWKFEYEGGQRDINQLHLPVNRDVKMIMSSQDAIHSFFVPAFRIKADVLPGR
                     FTTTWFRPTKVGTYHLFCSQYCGTDHSGMIGQVIVMEPAAYQAWMSGGASSGTLASNG
                     QQLFQQLGCATCHRFDTQGRAPNLTGVFGKPVLLEDGRTVIADENYIRESILIPGAKI
                     VSGFKPIMPTFQGQVSEENLMALVAYVKSLAQAQQKGAASDNSSVPDTPNSQ"
     gene            1876..>2064
                     /locus_tag="VFC15_17870"
     CDS             1876..>2064
                     /locus_tag="VFC15_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12081.1"
                     /translation="MATALQTVERENYLNSNYGIKSWLLTTDHKRIGILYLISITAMF
                     WLGGFFAMMIRLELLTPAG"
BASE COUNT          424 a          586 c          610 g          444 t
ORIGIN      
        1 cgcgaaagga ctggcaattc tggtgacaac cggcatctgt gccgctgccc agccgccttc
       61 ttcttttcag gacaagagct caggctccgc ttcccaggtc agtccagccg acctgtcgaa
      121 cgtcgggatc gagcagcggc tcgaccagca ggtaccgctc gatctgcagt tcaagaatga
      181 gacgggaaag acggtgcagc tcggcgacta ctttaagtca ggccggcccg ttcttttgaa
      241 cctggtgtac tacacctgtc ccatgttgtg tggggaggaa ttagcgggcg aggcgagtgc
      301 gctcggcgtg ctgcgattta cgccgggtaa tgagtacgaa gtagtatctg tgagcttcaa
      361 cccagatgag actcccaaag atgcgtccga gaaaaaacag gtttacatcg cgcgcatgaa
      421 tgagcatctg gagcacaaga ccaacggcga gggctggcat ttcctgacgg gtcagcagtc
      481 cgagatcaaa caactggccg atgcggtggg cttccactac cgccgcgacc cgcgcaccgg
      541 acagttcatt cacgccgccg ggatcatgat cgtgaccccg gccggcaaga tcgcccagta
      601 ctactacgga gttgaattct ctccgaagga cattcgcctg ggcttgatcg aggcttcgcg
      661 tgacaagatt ggcaccctgg tcgaccaggt aacgctgtac tgctatcact acgatcccaa
      721 gaccgggcga tacggcgcgg tagtcaccaa catgatgcgg ctggctgggg ccgcgaccat
      781 gctgattctc ggcggcttcc tcgtcgtgat gtttaagcgt gagtctaacg gcggcaaaaa
      841 cggaacagga cgggcccgat agttatgtgg aagaacctcc cactctttcc tgagcgtgcc
      901 tcggcactgg catggcaggt ggacggactt tattttctgc tgatcgcagt atcggcgttt
      961 ttcacgctgc tgatttttgc tttgatcttc gtcttcgcgg tcaagtatcg ccggtcggcc
     1021 catcctcatg cggtgcagat tgaaggctcg ctgccgctgg aactggcgtg gacgctgatt
     1081 cccctgggaa tctgcatgat cttcttcgcc tggggctcgc tgatctattt ccaggaggcg
     1141 cgcccgccga agggcgcgat ggaagtttac gccgtcgcca aacagtggat gtggaagttc
     1201 gagtatgagg ggggtcagcg agacattaac cagcttcacc tgccagtgaa ccgcgacgtc
     1261 aagatgatca tgagttcgca ggacgcgatc cacagcttct tcgtgccggc atttcgtatc
     1321 aaggctgacg tgcttcccgg acgatttacc acgacctggt tccgtcctac aaaggttgga
     1381 acttatcacc tattctgctc gcaatattgc ggcacggacc actccggcat gatcggccaa
     1441 gtcatcgtga tggagcctgc cgcgtaccag gcgtggatga gcggtggggc ttcctcgggt
     1501 acgctggcgt ccaacgggca gcagcttttc cagcagttgg gatgcgcgac ctgccaccgg
     1561 ttcgataccc agggacgcgc tcccaacctg actggcgtct tcggtaagcc ggtgctgctg
     1621 gaggacgggc gcacggtaat agccgacgag aactatattc gcgagtccat tcttattccg
     1681 ggcgccaaga tcgtttcggg tttcaagccc atcatgccca ccttccaggg acaagtttcg
     1741 gaagagaact tgatggcgct tgtggcctac gtcaaatcgc tggcgcaagc gcaacagaag
     1801 ggagcggcga gcgataactc gtccgttccg gatacgccga actcgcaatg aggcattaga
     1861 ccctaaaggt gtgacatggc aaccgcttta cagaccgtcg agcgcgagaa ctacctgaat
     1921 tcaaactacg gcattaagtc gtggctgctg acgaccgatc ataagcggat cgggatcctc
     1981 tacctgatct cgatcaccgc catgttctgg ctgggcgggt ttttcgccat gatgatccgc
     2041 ttggagctgc tgacgcccgc cggc
//
LOCUS       DASRKK010000743         8821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1056676, whole genome shotgun sequence.
ACCESSION   DASRKK010000743 DASRKK010000000
VERSION     DASRKK010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056676"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFC15_17875"
     CDS             complement(<1..471)
                     /locus_tag="VFC15_17875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM12082.1"
                     /translation="MVLTDKYGRSIHDLRISITDRCNYKCVYCRSGNDGPAFPEMPIA
                     DYLRMVGVFVSLGITKVRLTGGEPLLRRGLVDMVRELGQMKTLTGTPLDIAITTNGHL
                     LAELAQPLADAGLSRVTVSMDAVDEERFARITRVPNGYERVLAGVRAAQRAGLNP"
     regulatory      complement(604..743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(838..3996)
                     /locus_tag="VFC15_17880"
     CDS             complement(838..3996)
                     /locus_tag="VFC15_17880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM12083.1"
                     /translation="MLVSVSAFAQSTTEGAVAGTIYDPQGAVVAGATVTVRNNGTNQE
                     QTATTDSTGYFRITKLTPASYTVSVTAQGFAGYRAPQVIVEVGRVTEITPRLGISGSS
                     EKVEVTAEAPTVNTTSADFAPVLNSVAVENLPINGGRWSDFALLTPGVNNDINGFGLL
                     SVRGISTLMNNTTIDGANNNQAFFSEERGRTRAGYSTPKVAVQEFQVNTSNYSAEYGR
                     AAGGVINTVTKSGTNEIHGEAYFFYRDNAWGATNPFTTLATQTSPGVYTIEPFKPSDI
                     RRISGIGLGGAIIKDKLFWYFAYDWYYRNFPGTAVPFSASTFYAPPTTATIATLATRL
                     GVSTGQAQTDYTNGLNGLNSMLGTVPRTGEQYIWFPKIDWNINEKNHLSLSVNRMRWA
                     SPAGIQTQASNNYGIASFGNDYVKDTWGIANLNSFITTNLSNQFRFQYGRDFEYENNQ
                     IPTPYEQSTLVNPPGYTNPLGLPPYIQLASTSVGFNFGTANFLERPQYPNEYVQQYAD
                     TVTWLKGRHTFKFGTDINYTTDKTQNLRNQYGSYTYNSLVDYFSDLYATPGAKVCTAT
                     VSGKLVPAPCYSNFTQAFGPLGLQFSTTDLGFFAEDTWKATPRLTLTLGLRYEYEMMP
                     STQLANPAVPATSQLPSDKNNWGPRVGFAYDVFGDGNTSLRGGFGMYYGRIINSTIYN
                     ALINTGVSAGQLSFSYTNTQGGAAFPQIFASTPTGTLKPSLVFFNKGFQNPLVYESDL
                     SIQHEFGKSVVLSLNYLGSFGRELPDFVDTNLPTSTSTLTYTVVDPTGKGPLAAGSTI
                     TTPLYTGTRPNTNYGAMTDIFSGVNSSYNALAVLLNKRFSNNIQFNVNYTWSHSIDFG
                     QNETTFTDTNDLLFPNCMKCEYGNSNFDVRQRFIISGVFDAPWKVKGFAGYLANGWEL
                     APIYQAQSGLPYSLVTSGNAPGGLAGGSYNGSGGANRLLVTGRNTYRYPRTQVVDLRL
                     SKSIPFKERYQLQILAEAFNLANHVNVTGVANSAYSITGSTLVFNTPSTNGKAMLGTV
                     TNANSNFAYSPRNIQIGAKFTF"
     gene            complement(4374..6089)
                     /locus_tag="VFC15_17885"
     CDS             complement(4374..6089)
                     /locus_tag="VFC15_17885"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZM12084.1"
                     /translation="MIVFRKPLPNIERPAMEADVVIVGGGPAGMACALRLSQLIDEHN
                     AKHPDAPLTKDNIYVLEKAREAGAHCLSGALLDPRSMLELLPDFENDAPLEAAVVKEA
                     VYYLTRTGKFKLPIVPPPMRDHGNYVISINRFVKWLASKVEAAGITIFTGFAGTELLF
                     EKGRVLGVRTDDKGVDKNGEQKSNFEPGYDLKAKVVILAEGSRGSLTKQLIGRFQLDN
                     ERNPQTYGIGVKELWELPPGRLAAGEVIYTMGYPLTFNEYGGAWIYGSHDNLVSLGFV
                     TGLDYPDPRIDPQRVLQEFKKHPFIAKLLEGGKMVRYGAKSLPYGGWWCIPPVAGDGW
                     MILGDSAGFLNSQRLKGIHLAIKSGMLAAETAFEAMAKDDYSLDVLEKFQERVDKSWI
                     KEELWPVRNFHQGFDKGFLSGMLHAGIQQATGGRGVRAHYSNHAGHLRMKQLSQLPAD
                     GGAEAHIINAAKGDGKLTFDKLTDVYYSGTKHEEDQPAHLVIHDTNICNERCVREFGN
                     PCKNFCPASVYEMVEAADTPSGLQIHLNASNCVHCKTCDIMDPYEIITWVPPEGGGGP
                     NYEGM"
     gene            complement(6086..7078)
                     /locus_tag="VFC15_17890"
     CDS             complement(6086..7078)
                     /locus_tag="VFC15_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZM12085.1"
                     /translation="MADTILVVVEQRDGKLNRVSWETLTGAQAIAAETGWPLEAAVVG
                     SGVGDIANEVAAKRVGKVYDIESSKLEPYTPDGFVSALKGFIEQKQPKLVLMPHTYQV
                     RDFAPKLATALNRALISDAIGYKYEGGKLLFTRQMFQGKFVADVSFTGDAPYFATFQS
                     GAYRGDKVEDGSAAAPVEKVDVAIAEGVIRNKPEAPFKEAKQAVDLTQAEIIVSVGRG
                     IKEQKNITLAEQLAAALGGEIAASRPICDSGWLPMDRQVGSSGQTVAPKLYLALGISG
                     AIQHVVGMKGSRSIIAINKDSEAPIFEIADIAVVGNLFDIVPPLIEEIKKAKGA"
     gene            complement(7209..7475)
                     /locus_tag="VFC15_17895"
     CDS             complement(7209..7475)
                     /locus_tag="VFC15_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12086.1"
                     /translation="MVKMVVYEGKLVSRKQEAVMPSKEESRETGKAFMVVGWVLVLFA
                     VLVMFFEPAAFRLGQMRFGAIAIALVVIGLLLNFYGYRLKRRSP"
     gene            complement(7484..8254)
                     /locus_tag="VFC15_17900"
     CDS             complement(7484..8254)
                     /locus_tag="VFC15_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZM12087.1"
                     /translation="MKILVCMKQVPQKDAPLKLNESGTWIREDVSYEVNEPDAFALEE
                     ALRQREKHGGEVVVITSGPTRAGQVLREALAKGADRAIHLEGDSFVGLDAFNTAKAFA
                     GVVKDENFDLIFTGLQSDDYGFAQTGVLLAELLGWSHATIIMQIEKTDSGIRVKRELE
                     AGYFQFVDMPLPAVLTIQSGINKLRYATLIGIKQAKNKPMKKVTFDDVKAALGDNLQK
                     IEKLYIPLKTKKTEVIEGPPAEVAKKLVDKLRNEIHVL"
BASE COUNT         1806 a         2515 c         2570 g         1930 t
ORIGIN      
        1 cggattcaat ccagcacgct gggcggcgcg gactcccgcc aatactcgtt cgtatccgtt
       61 gggcacgcgc gtaatgcggg caaagcgctc ttcatcgacc gcatccatcg agaccgtcac
      121 acgcgaaagt ccggcgtcgg ccagcggctg agccagttct gccaacaggt gtccgttagt
      181 agtgatggcg atgtcgagcg gagtgccggt gagcgtcttc atctgtccaa gctcccgcac
      241 catgtcgacc agacctcggc gtagtagcgg ctcaccgccg gtgaggcgga ccttggtgat
      301 ccccagactg acgaagaccc ccaccatgcg caggtagtcg gcaatcggca tctctggaaa
      361 tgcggggcca tcgtttcccg agcggcaata aacgcacttg tagttgcagc gatcggtaat
      421 ggatatccgc agatcgtgga tgctgcggcc atatttgtcg gtaaggacca ttccgtcttc
      481 gctttcacca cagagacacg gaggcgcgag aagtcccacg aacctctgtc catatggtgc
      541 gttcgagaat ccaaaactgt ttggagaagc gcaacgaaag gaagccgggc gctcgcaggt
      601 tggcgccacc gctgatcgtt tcctttccaa gcacgggagg cgtggacgtt tccctccaca
      661 ccctcggccc cacttgcgtg gggcgccgcc gccgcagcct tgggatttcc ggtaggccgc
      721 ggaggtcgga aacagtatcg gtaattatag atgcaaatgc gggcctgaga gcgcacagaa
      781 aaacgcgccc cggcggggat ggccggggcg cgaatcgatt gcaatcgcat cggaagccta
      841 gaaggtaaac ttggctccga tctggatgtt gcggggcgaa taggcgaagt tgctattcgc
      901 gttggtcacg gtgccgagca tggccttacc gttcgtgctc ggggtgttaa acaccagggt
      961 gctcccggtg atggagtacg cgctgttggc aacaccagtt acgttgacgt ggttagccag
     1021 gttgaatgct tcggccagaa tctgaagctg ataccgctcc ttgaaaggaa tcgacttcga
     1081 gagccgcaga tcaaccacct gcgttcgggg atagcggtag gtgttgcgtc cagtcaccaa
     1141 gaggcgattc gctccgccgg aaccgttata gctaccgccg gcgaggcccc caggagcgtt
     1201 accgctggtt acgagcgagt agggaagccc ggactgcgcc tggtaaatcg gcgccagttc
     1261 ccagccgttg gccaggtaac ccgcgaatcc cttgaccttc catggggcat caaacacccc
     1321 gctgatgatg aagcgctgac gaacatcaaa gttggagttc ccatactcgc acttcataca
     1381 gttcgggaac agcaggtcgt tggtatcggt gaacgttgtt tcgttttggc cgaaatcgat
     1441 ggagtgcgac caggtgtagt tgacgttgaa ctgaatgttg ttgctgaagc gcttgttcag
     1501 gagtacggcg agagcgttat agcttgagtt gacgccgctg aaaatgtcgg tcatagcgcc
     1561 ataattggtg ttagggcggg taccggtgta cagcggtgtg gtgatcgtcg aaccagcggc
     1621 aagcggtccc ttccctgtgg gatccacgac cgtgtaggtc agtgtggaag ttgatgttgg
     1681 gaggttagtg tcaacaaagt cgggcaactc gcgcccgaaa cttcccaggt aattcagcga
     1741 gaggacaaca ctcttgccaa attcgtgctg gatcgacaag tcggattcat acaccaaggg
     1801 gttctggaac cccttgttga agaatacgag actcggtttg agcgtacccg tcggtgtcga
     1861 ggcaaagatt tgtgggaacg ccgccccgcc ttgcgtattc gtgtagctga aactcaactg
     1921 cccggccgag acaccggtgt tgatgagcgc gttgtaaatg gtggagttaa taatgcggcc
     1981 gtaatacata ccgaacccgc cgcgcagcga tgtgttgccg tcaccgaata cgtcataggc
     2041 aaagccaacg cgtggacccc agttgttttt gtcgctgggc aactgggacg ttgccggcac
     2101 agcggggttg gccaactggg tggacggcat catctcgtat tcgtaacgca atcctagtgt
     2161 cagggtcagg cgaggggtgg ccttccacgt atcctccgcg aagaaaccca gatcggtggt
     2221 agagaactgc aagcccagag gcccgaatgc ctgagtaaag ttggagtaac acggcgccgg
     2281 aacgagtttg cccgagacgg tggccgtgca taccttcgcc ccgggggtcg cgtagaggtc
     2341 agagaagtag tcaaccaggc tgttgtaggt gtacgaacca tactggttgc ggaggttctg
     2401 cgttttgtct gtcgtgtaat tgatgtcggt gccgaacttg aaggtgtgtc tacccttcag
     2461 ccaagtgacc gtgtcggcat actgctgtac atactcgttg ggatactgcg gcctctccag
     2521 gaaattcgca gttccaaagt taaaaccaac cgaggtgctg gccagttgga tgtaaggcgg
     2581 cagtcccaga gggttggtgt atcccggagg atttaccaac gtgctctgct cgtaaggcgt
     2641 ggggatctgg ttgttctcgt attcaaagtc acgcccgtac tgaaagcgga actggttgga
     2701 gagattggtc gtgatgaaac tgttcaggtt ggcaataccc caagtgtcct tcacgtaatc
     2761 gttgccaaag ctggcgatgc cgtagttgtt gctggcttgg gtttgaatac cggcgggcga
     2821 agcccatcgc atgcggttga ccgacaacga gaggtggttc ttttcgttga tgttccagtc
     2881 gatcttgggg aaccagatgt actgctcgcc cgtgcgcggc acggtgccca gcatgctgtt
     2941 caggccattc agcccgttcg tgtagtcggt ttgcgcttgg ccggtactca cgccgagccg
     3001 ggttgctaga gtcgcaatcg tagctgtcgt aggcggggca tagaacgttg aagcgctgaa
     3061 cgggacggcc gtaccgggga aattcctgta gtaccagtcg tacgcgaaat accagaacag
     3121 cttgtccttg atgattgcgc cgccgagacc gataccgctg attcggcgaa tatccgaagg
     3181 cttgaatggc tcgatcgtat acactcccgg cgacgtctgg gtcgcgagcg tggtgaacgg
     3241 attcgtcgca ccccatgcat tgtccctgta gaagaagtaa gcctcgccgt ggatttcgtt
     3301 ggtgccgctc ttcgtcactg tattaatgac gccaccagca gcgcgcccgt attcagcgga
     3361 atagttcgag gtgttgacct ggaactcctg caccgcaact ttgggagtgg agtacccggc
     3421 gcgtgtgcgg ccacgttcct cagagaaaaa agcctggtta ttgttggcgc cgtcaatcgt
     3481 ggtgttgttc atcagtgtgc tgataccgcg cacactgagc agcccgaatc cattgatgtc
     3541 gttgttcacg cccggcgtca ggagggcgaa atcagaccag cgtccgccat tgatgggcag
     3601 attctcaacg gccaccgagt taagcactgg ggcgaagtcg gcagacgtgg tgttcactgt
     3661 gggcgcttcg gccgtaacct ccactttctc ggaactgccg ctgattccca gtcgcggcgt
     3721 gatctctgtc acgcgaccga cttcgacgat gacctgcgga gcacgataac cggcaaagcc
     3781 ctgagcggtg acgctgacgg tatacgatgc cggagtcagc ttggtgatac ggaagtatcc
     3841 cgtggaatcg gtcgtagctg tctgctcctg gtttgtccca ttattgcgga cggtgaccgt
     3901 agcgcctgcg acaacggcgc cttgcggatc gtaaatcgtt ccggcaaccg caccttcggt
     3961 cgtggactgg gcgaatgccg aaactgaaac cagcacgaaa actgcaagca ggaaagttgt
     4021 gaaaagggtt gtgagtttag aaaatgacga agtttcgaaa ggatggctgc agccagaccc
     4081 caaatgcatg gcaattcgct cctgatgaaa tttggcaccc cagtacgccg ccgggcgcac
     4141 cggttatttc cacttggaac aaaaatcccc gacgcagtac ttagtagcgt aacagtcttg
     4201 cggtaggaga ttcattacag aaatatttat gtctgttgaa aaaaatatct atggcgctta
     4261 tgaatcagca ggcctgtagg cgagtggccc gatccaacga gaaaagcctc gccgacggct
     4321 cggcgaggcc ctgtggaaaa ctcggactcg atatttaggg ctcgaaaaat ccgctacata
     4381 ccctcataat tcggaccgcc gccaccctcc ggcggtaccc aggtaatgat ctcgtaggga
     4441 tccatgatgt cgcaggtctt gcagtgcaca caattcgatg cattcagatg aatctgcaac
     4501 ccgctgggag tatcggccgc ctccaccatt tcatacacac tagcaggaca gaaatttttg
     4561 caagggttgc caaattcccg cacgcaacgc tcgttgcaga tgttggtgtc gtggatgacg
     4621 aggtgcgctg gctgatcttc ctcgtgcttg gtgcccgagt aatacacgtc ggtcagcttg
     4681 tcgaaagtga gctttccgtc gccttttgcg gcattgatga tgtgcgcttc cgctccgccg
     4741 tccgcaggta gctgcgagag ttgcttcatg cggagatgcc cagcatgatt gctgtagtgc
     4801 gcgcgcactc cacggcctcc ggtggcttgc tggatgccgg cgtgcagcat gcccgacaga
     4861 aagcccttgt cgaagccctg gtggaaattg cggactggcc acaactcctc cttgatccag
     4921 ctcttgtcca cgcgctcctg gaatttctcg aggacgtcga gcgagtagtc atctttcgcc
     4981 atcgcctcaa aggcagtttc ggcggctagc atgccgcttt tgatggcgag atgtattcct
     5041 ttcagtcgct gcgaattcag gaaaccggcg gagtcgccca ggatcatcca accatccccc
     5101 gcaaccggcg ggatgcacca ccagcctccg taggggagag acttggctcc gtaacgaacc
     5161 atcttgccgc cttcgagaag tttcgcaata aacggatgct tcttgaattc ctgcaggacg
     5221 cgttgcggat cgatacgagg gtcaggataa tccagaccgg tcacgaagcc cagcgacacc
     5281 aggttgtcat gcgaaccgta aatccaagcg ccaccgtact cattgaaggt cagcggatag
     5341 cccatggtgt agatcacttc acccgccgca agccgtcccg ggggcaactc ccaaagctcc
     5401 ttgacgccga tgccgtaggt ctggggattg cgttcgttgt cgagctggaa gcgaccgatc
     5461 aactgcttgg tcaacgaacc gcggctgcct tcggcgagga tgacaacctt ggccttcagg
     5521 tcatagccgg gctcgaagtt cgatttctgc tcgccatttt tatcgacgcc cttgtcgtcg
     5581 gtgcggacac caagaactcg acccttttcg aaaagcagct cggtcccggc gaacccagtg
     5641 aagatcgtga tccctgccgc ttcgaccttg ctggccagcc atttcacgaa tcggttgatg
     5701 gagatcacat agttgccgtg atcgcgcatc gggggtggca cgatggggag cttgaatttc
     5761 cccgtgcggg tgaggtagta aacggcttct ttcaccaccg ccgcttcgag cggcgcgtcg
     5821 ttctcaaaat ccggcagcag ctcgagcatg gaacgagggt cgagcagtgc gccggaaaga
     5881 cagtgcgcac ccgcctcgcg cgctttttcc agtacgtaga tattgtcctt ggtgagcgga
     5941 gcgtcgggat gcttggcgtt gtgttcgtcg atcaactgcg acaagcgcag cgcgcaggcc
     6001 atgccggcgg ggcccccacc gacgatgacc acgtccgctt ccatcgcagg acgctcgata
     6061 ttcggcagtg gtttacggaa tacgatcatg cgcccttcgc cttctttatt tcctcgatga
     6121 gcggcggcac gatatcgaac aggttgccca ccaccgcgat gtcggcgatc tcgaagatcg
     6181 gcgcttcgga atccttgttg atggcgatga tgctgcgcga tcccttcatc ccgaccacat
     6241 gctgaatcgc accgctgata ccgagagcga gatacagctt aggagcgacc gtctgcccag
     6301 atgagccgac ctgacggtcc atgggcagcc agcccgagtc gcaaatcggc cttgatgcgg
     6361 caatttcgcc acccagcgcg gctgcgagct gctcggccaa ggtgatgttc ttttgctcct
     6421 tgatgccgcg gcccaccgag acaataatct cggcctgagt gagatcgacg gcctgcttgg
     6481 cttccttgaa tggagcctcg ggcttattgc ggatgacacc ttcggcaatc gcgacatcga
     6541 ccttttcgac gggagctgct gcgctgccgt cttcgacctt gtcgccgcga taggcgcctg
     6601 actggaacgt cgcgaagtaa ggagcatcgc cggtgaagga aacgtcggca acgaatttgc
     6661 cctggaacat ctgccgggta aacagcaact tgccaccctc gtacttgtag ccaattgcgt
     6721 cgctgatcaa cgctcgattc aaggccgtgg ccagcttggg agcgaagtcg cgcacctggt
     6781 aggtatgcgg catcagcacc agcttaggct gcttctgctc gatgaagcct ttcagcgccg
     6841 acacgaatcc atcgggagtg taaggctcaa gcttcgacga ctcgatgtcg tacaccttgc
     6901 cgactcgctt ggccgcaact tcgttcgcta tgtccccaac tccgctgccc acaacggccg
     6961 cttccagagg ccacccggtt tcagccgcga tggcctgtgc gcccgtgagc gtttcccacg
     7021 aaacgcggtt caatttgccg tcccgctgct ctactacaac cagaatagtg tctgccattt
     7081 cgatccgttc ctgcgccctg ttttgagcgc gtgccgtgcg ctactgctgt ccggcacaag
     7141 ttagtcctct ggactgcgac ccacgggctt gtttgcccgt ctgtcgtttg acgtcgcgtc
     7201 ctgcatattc agggactgcg ccgctttaat cgatagccgt aaaagttcag caacagccct
     7261 atgacgacca gtgcgatggc aatggcgccg aaccgcatct gtccgagtct gaacgctgcc
     7321 ggctcgaaaa acatcacgag caccgcaaat agcaccagga cccagcccac gaccatgaag
     7381 gcctttccgg tctcgcggct ctcttctttg gatggcatca ccgcctcctg cttgcgagat
     7441 accaacttcc cttcgtaaac gaccatcttg accattcacc cgatcacagc acgtgaatct
     7501 cgttacgcaa cttatcgacc aacttcttgg ccacttccgc cggcggacct tcgatcactt
     7561 cggtcttctt cgtcttcaga ggaatgtaca gcttctcgat cttctgcagg ttatcgccga
     7621 gcgcagcttt cacgtcgtca aacgtgactt tcttcattgg cttgttcttc gcctgcttaa
     7681 tgccgatcag cgtggcatac cgcagcttgt tgatgccgga ctggatggtc agcaccgcgg
     7741 gtagcggcat gtcgacaaac tggaagtatc ccgcctcgag ctcgcgtttc acccggatgc
     7801 cggaatccgt cttttcgatc tgcatgatga tggtggcatg cgaccagccc agcagttccg
     7861 ccagcaagac gccggtttgc gcgaaaccat agtcatccga ttgcaggcca gtgaaaatca
     7921 gatcgaaatt ttcgtccttc accactccgg caaacgcctt ggcggtattg aacgcgtcca
     7981 acccgacgaa gctgtcgcct tccaggtgga tagcacggtc agcgcccttg gccagcgcct
     8041 cgcgcagcac ctgtcccgcc cgggtggggc cgctggtgat aaccacgacc tctcccccat
     8101 gcttttccct ctggcgcagc gcctcttcaa gcgcgaacgc atccggctca ttaacttcat
     8161 aggaaacatc ttcgcgaatc catgttccgc tctcgttcaa cttgagcgga gcgtccttct
     8221 gcgggacctg cttcatgcaa accaggattt tcaccctgag ctccttcgac ccgggcaatt
     8281 tgccgtcggg cagcccggtt catgcggcgg acaccttacg ctccgcctgc ggcgttggtg
     8341 atatcggaat ccgatgcagc tcgctacaca gcgaatcgat catcactatc gaaacagccg
     8401 agtataaatg actggcagga agatgcgaaa ggacatgcga ccgcagtttc aattcgggaa
     8461 aatgcgctcg cgattcaaat gcagcgagat tgtggaaatt ttccgcttgt gagagtgcgc
     8521 gagccgctca cgacccgcgc cgttcttgcg ggtgccccag agcctgccct gagcttgtcg
     8581 aagggttcgc gcccgttacg aaactcgcgc ttcgttttgc ggtctggaac aaacgctaca
     8641 aatgcgagcg ccggaggcgc gaaatagtct agcccagcac gcagccggac ggggtccccg
     8701 acccgcgccg cttttgcgcg gattggggta ggtaggcgga gtgctgggga gagtgggaga
     8761 attcatggga gtgccgtagg cacggcagcg tttcgtaaca cccaggttcg cgcccgttac
     8821 g
//
LOCUS       DASRKK010000744         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_469861, whole genome shotgun sequence.
ACCESSION   DASRKK010000744 DASRKK010000000
VERSION     DASRKK010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469861"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..436
                     /locus_tag="VFC15_17905"
     CDS             182..436
                     /locus_tag="VFC15_17905"
                     /inference="COORDINATES: protein motif:HMM:NF022969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HZM12088.1"
                     /translation="MNRVPDSELILVPGGTLITATPEEGRALAITMARHTIHKIQPDL
                     DVLKGGRPNYGANPDGLIAASQVVAIEFQTIAAANKYWQK"
     gene            535..1338
                     /locus_tag="VFC15_17910"
     CDS             535..1338
                     /locus_tag="VFC15_17910"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM12089.1"
                     /translation="MIQKQRSIVLIHGLWMTSLSWENWVTRYAAHGYSVVASSWPGMD
                     GDIEQLRRDPSAIATLGIDTIVEHYEKIIRNLDSPPLIIGHSFGGLITQILLDRGLGA
                     AGVAIAPAPVKGIIFLPLSTLRVSFPALSNPANNHRAVPLTAEQFHYAFTNNLSEQES
                     LAVYERYAVPGPDHVLFQAAFANFNPHAATAVDFHNDHRAPLLLISGGNDHVSPASVV
                     EANFKLYDKSKAVTEYKNFAERTHYTLGQDGWEQVADFAVTWAESKALV"
     gene            complement(1607..1810)
                     /locus_tag="VFC15_17915"
     CDS             complement(1607..1810)
                     /locus_tag="VFC15_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12090.1"
                     /translation="MNIRHLVLSVSRWLLSMGPLVLVAGLQQPVHASDADLSKFKRIE
                     PQFIAALGDPAASRVVHRRTTGC"
BASE COUNT          487 a          597 c          600 g          428 t
ORIGIN      
        1 accgcgggga agtttgttgt tgacatcgaa ttgcgttaga cggcggcgga tgcgatatgc
       61 ccgaccgaga cactgttcgt ctcagacaat aggattgata tttcgcacgg aagcgaagta
      121 ccgagcgaag agtcgaggca gaccgattag agatttctaa gaaccaacat aggagagttt
      181 tatgaatcgc gtaccagatt cagagttgat tttagtccct ggtggaacat tgataaccgc
      241 cactccggaa gaggggcgcg cgctcgcaat caccatggct cgccacacaa tccacaagat
      301 tcaaccggat ctcgacgttc tgaagggtgg tcgccccaac tatggtgcga acccagacgg
      361 cctcatcgcg gccagccaag tcgttgccat cgagttccag accatcgcgg cggccaacaa
      421 gtactggcag aaataggtcg cttccgaaaa cggaggaggc ctcgatctcg gtacctgcaa
      481 aagatgttaa gcccgcggac gcatgcgggc aaaatgattc aaaaaggagc tattttgata
      541 cagaaacagc gctcaatcgt cctgatccat gggctttgga tgacatctct cagttgggag
      601 aactgggtta cccgctatgc tgctcacggg tattccgtcg ttgccagtag ctggccggga
      661 atggatggcg acatcgagca acttcggcgc gacccttctg cgattgcaac gctcggaatc
      721 gataccatcg tcgaacacta cgaaaagatc attcgcaatc tggacagtcc gccgttgatc
      781 atagggcatt ccttcggtgg actgattacc cagattctac tggaccgcgg cctgggcgcc
      841 gcgggtgtcg ccattgcgcc agcacccgtc aaaggcatca tctttctccc tctatcgacg
      901 ctcagagtgt cttttccggc tctgagcaat cccgcaaaca accatcgtgc cgtgccactc
      961 acggcagagc agtttcatta cgctttcacg aacaatctga gtgaacagga gtcgctcgct
     1021 gtatatgagc ggtatgcggt gcccggcccc gaccacgtgc tgttccaggc cgctttcgcc
     1081 aatttcaatc cgcatgctgc taccgcagtc gattttcata atgatcaccg tgcgcccttg
     1141 ctgctcattt ccggcggcaa cgatcacgtc tcgccggcat cggtggtcga ggcaaatttc
     1201 aaactgtacg ataagtcgaa agccgttacc gaatacaaga acttcgcaga acggacgcac
     1261 tacacgctgg ggcaggacgg gtgggaacag gttgccgact tcgcggtgac ttgggcagaa
     1321 agcaaggcac tagtttgacg attgtatcgc aggcaagtgg tgtgcccgga gaatcagagc
     1381 tctccggaaa gtggacctcc acgggcccgt cctcgggaaa ttgacgacgg ggcgagacct
     1441 ccgggtggat cacgcgccaa acggactgag cgcgctacca cgacgatgga tcagtggcac
     1501 aggagcccac cgatcatgaa gcgtaagagc aaaaccaacg gaaagcgcgg gacgccgaag
     1561 tccatactga gactgcccga cctcgaacat gcgaagtctg ccgtcattaa cagcctgtcg
     1621 tcctccgatg cacaacgcgg ctggccgcgg ggtctcccag cgccgcgatg aactgcggct
     1681 cgatgcgctt gaacttcgag aggtctgcgt ctgacgcgtg aaccggctgc tgcaggccgg
     1741 ccaccaacac gaggggtccc atggacagga gccagcggct cacggacagc acgaggtggc
     1801 ggatattcat acgattgctc ctcttggacg acttgataac atcctacatc aacgaggggc
     1861 gacgatgaac gagagatggg ttcgggtggt ccggtcaagg cggtgctcaa acgctgaaag
     1921 cactacacct ggaaactctt ctggtaccca agttccctgg agcgtcttcc gccgtctatg
     1981 gcgagcttcc ttaagcgcac cagcgacgcc tcttttgtgg cctaggctct tgtcgataag
     2041 tccctctatg tcggccagcg tttaccgagg tcttcgcggt tgcaatttga gatgccgcgg
     2101 gcggataacg ca
//
LOCUS       DASRKK010000745        21024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_273259, whole genome shotgun sequence.
ACCESSION   DASRKK010000745 DASRKK010000000
VERSION     DASRKK010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273259"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..8176
                     /locus_tag="VFC15_17920"
     CDS             68..8176
                     /locus_tag="VFC15_17920"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZM12091.1"
                     /translation="MQLVQRLRDVNPNMGAVLRWLDQRLAEQGTTADEIVRTEYQEQT
                     AMNVTVRNIIPSMRLMMDFDWNDFVESISLVDEVLRSETNFDEMDFATRDTYRHAIED
                     LSRGSDHKEIEVAEHVVHRIKRARTAPPGDGQPRGDRSTDPGYYLISTGRIAFERELG
                     FRVPWKTKVLRFYMRSATLSYLGTIALGTALIVALPLLHERKAGVAGWGLWLLGLLAV
                     VPASDLAIALINRFVTDLLGPRMLPRLELRDGVTPQLRTMVVVPTLLTNQHDVEQLVE
                     RLEVHYLANSEGDLRFALLTDWMDAASETIPADNELLALAVDGVARLNKIHGPTPDGD
                     ARFQLLHRKRVWNESEAKWMGWERKRGKLVELNQLLRGGMNTTFLPVGGRPAQAPAGV
                     RYVITLDADTRMPRGVACRLVGTMAHPLNQPRFSAGHGRVVEGYGIVQPRITHSLTAD
                     HERSFFQALFAGPSGIDPYASAVSDVYQDLFEEGSFTGKGIYDVDAVEKAMDGKVPEN
                     ALLSHDLFEGEFARTALASDIELFEEYPSHYEVAAARQHRWVRGDWQLLPWIMGRPRA
                     RSEGHPKVAIPAIGRWKMLDNLRRSLSAPAAFFALIAACLIPAASSWVWARFILATMA
                     IPALLSFLIGINPPRRGISKRSHFRSMLSDLSLGASQLGVTITMLAYQAWLMLDAIIR
                     TLVRMTVTHRHLLEWVTAARAKHAVDLTITGVYRRMAGGVLFAMAALVIVASWGHRGW
                     GVLLPFIALWLAAPAVAWQISLPPRRSGAEPLSAANARALHLITRRTWRFFEIFIAPA
                     DHALPPDNFQEEPTPVIAHRTSPTNIGLYLLSTQAAHDLGYLGTLDTVDRLEATLGTL
                     SQLELVRGHFYNWYDTRDLRPLDPKYVSSVDSGNLAGHLLALANGCRELIQKSSLGTS
                     TLGGVQDTIELLREAVSQIAETRRTNTVTRKQLSNAIDALANSLELAPINPAEWAIRL
                     AELRTRAVTVADIAQTMAQERGDASDSELGVWADAVKACVESHIRDAEILLPWLRRDS
                     VSNPAITRRSQEQAPEWVAIEPFFGSVPKLADIPERTEEAQRALALLRTSLVNDSSAN
                     FEILARVDALTQSLRQSGKDAIALARRLSAIAQKADDMFQAMDFSFLFDPTRKQFSIG
                     YRVAENKLDANCYDLLASEARLTSFIAIAKGDVPSSHWFRLGRALTPVGRGSALISWS
                     GSMFEYLMPALMMVSPTGSLLNQTYELVVRRQIAYGAERGVPWGISESAYNARDLDFN
                     YQYTAFGVPGLGLKRGLSEDLVIAPYATALAAMIDPNAAAENFAQLATAGGKGVYGFY
                     EALDYTGERLPEGENVAVVRAYMAHHQGMSLVALANVLSDGAMRDRFHAEPMVQASEL
                     LLQERTPRDVLVARPRAEEVSAAAHVREMVPPVVRRFSSPHELIPRTHLLSNGRYGVM
                     VTTAGSGYSRWQNLAVSRWREDVTRDCWGSYIFLRDGQSGQIWSAGYQPSGVEPDSYE
                     ASFAEDRAEFIRRDGSLVTTLEVVVSPEDDAEVRRVSIANLGTRTREVQVTSYAEVVL
                     APQAADEAHPAFSNLFVETEFVASAEVLLATRRRQSERDTPAWLAHVAMIEGESVGEL
                     QFETDRARFLGRGHSVREAISIADGRPLSNTAGPVLDPVMSLRRTVRIPPGGTARIAF
                     STLVAAAREQVLDLADKYRDARTWERTVMAAWTQAQVQLRHLGITPDEAHLFQRLANG
                     IVYSDPSLRPSADMLSRTTIDLPALWAHGISGDLPIALVRIDEAEDVGIIRQLLRAQE
                     YLLTKGLSVDIVILNEKPPSYEQELQGSLDDLVRAHRQRVSSEHGEVHGNVFLLRGDL
                     ISVQERALLQSAARVVLLSRHGTLAEQVIRSQRSETLTRPAPRPPRLGKDAEVPLDAP
                     DLEFFNGLGGFAEGGSEYVTVLGEGLRTPAPWINVIANPSFGFLVSESGSGYTWSGNS
                     HENQLTPWSNDPVTDPPGEAIYLRDEETGAVWSPTALPVRDEASGYAAWHGQGFSRFR
                     NGSQGIVSELLQYVPIRDSIKISRLVLQNRSARTRRLSVTAFAEWVLGSSRGITAPHI
                     ISELDSETNALLARSGWGGEFGGRIAFLDLCGRQTSWSGDRTEFLGRNGVTARPAALQ
                     RGGPLSGKVGASLDPCAALQTVVELRPDERVEIVVLLGQGKDRQQARELINCYRAANL
                     DHVLAEVTRQWDEVLGSVQITTPDRSMDILVNRWLLYQTLSCRVWGRAAFYQTSGAYG
                     FRDQLQDVMALTVAKRDVAHAHLLRAASRQFIQGDVQHWWHPPSGRGVRTRISDDLLW
                     LPYAVIHYIEVTGEWGLLDEAVPFLEGDLLAEGQLESYFQPRVSETRASLFEHCARAL
                     DRSLAVGIHGLPLIGTGDWNDGMNRVGQDGKGESVWLGWFLHTILWEFAKVADARGER
                     KRAETWRLHVSALKAALERDGWDGEWYRRAFFDDGTPLGTAQDKECRIDSIVQSWGVL
                     SGTAEPARAARAMAAVEKYLVRRPEGLVLVLTPPFDKTDHDPGYIKGYLPGIRENGGQ
                     YTHAAAWTVLASAALSDGDKAVELFRMLNPINRTSSRATVQRYKVEPYVAAGDVYSES
                     PHVGRGGWTWYTGSAGWLYRGAIEWLLGFRLRGTVLSIDPCIPRNWPSYSIRFRYHST
                     IYNIKVENPSGVSRGVALAEMDGARLLNSPNVPLVDDAKEHQVRIVMG"
     gene            complement(8278..8997)
                     /locus_tag="VFC15_17925"
     CDS             complement(8278..8997)
                     /locus_tag="VFC15_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12092.1"
                     /translation="MKDVVANELTDRTEHSNWMYRVTKVVEQQTLTQLEVETKDGPVH
                     RLLSINDAPLDSAQQQRETTRQEQLVREPSQQLAVKKQNDADELRLENFVRLLPSAFL
                     FQYDGWDGPDLRLSFRPDPAFVPLTMESKALQSMAGTILVSPQQKRLARMNGRLVENV
                     DFGFGILGRIAKGGTFEIERIQVSPSHWKTQLVDIHVDGRMLLFKTVSKQQHETRSDF
                     ESVSKDLDLSHGEALLQSRPN"
     gene            9331..10623
                     /locus_tag="VFC15_17930"
     CDS             9331..10623
                     /locus_tag="VFC15_17930"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZM12093.1"
                     /translation="MASNCRTHMIRMLALLLVTLMFSGHGTAYSVLTHEEIVDLLWAD
                     QIRPLILKRYPGLSEEQIKEAHAYAYGGAVIQDLGYYPFGSVEFSDLVHYVRTGDFVR
                     ELIAQSNDANEYAFSLGALSHYASDIAGHPAVNQAVAIEYPKLRAKYGASVRYAQDKT
                     AHLKTEFGFDTVQVAKNRYASQQYHDFIGFQVSKPLLERVFPIVYGLELKDVLPREDL
                     AIGSYRYSVSSLIPEMTQVALQTHKKEFMSETPDFAKQKFLYRISRSEYEKEWGKDYV
                     KPGLGTRILSTLLRYMPKIGPFKKLAFNSPTTQTEDMYIKSINTTVEQYRALLIAERA
                     DKLVLPNYDFDSGNPTKIAEYSLADDTYEKLLGQLAKSKFDRTTPELRDNVLLFYSDF
                     AASNESKDDKVSSANLMEWLGQLRAAKLVPATEAVASR"
     gene            complement(10640..11518)
                     /locus_tag="VFC15_17935"
     CDS             complement(10640..11518)
                     /locus_tag="VFC15_17935"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZM12094.1"
                     /translation="MIYRIVHRTTYKYKYPVSAGYHVACLKPRSSLHHQLAQTELCIQ
                     PTPATLTERLDYFGNLLCYFTIQEPHKELVVEARSEVTIGGNGRRLLQPSIPWEEAAR
                     LLPNDHSPAGLEAYQFGFESPRIRIRPEYAAYALQSFTPRRPMPEALRDLTARIYRDF
                     RFDAKVTNVRTSTEEVFRKRRGVCQDFAHLQIACLRSLNLAARYVSGYLRTYPPPGQP
                     RLVGADASHAWVSAYCPGIGWLDMDPTNNLVPSDGHVTLAWGRDYGDVSPLHGLILGG
                     GSHLLKVAVDMEPLDC"
     gene            complement(11515..14013)
                     /locus_tag="VFC15_17940"
     CDS             complement(11515..14013)
                     /locus_tag="VFC15_17940"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZM12095.1"
                     /translation="MTWDLYQSPLRYGDSYDELSADGVTPRPHWAHLMESLRAIGREE
                     LGRRWGRAERRIRENGITYNVYSDPLGVNRPWTIDIVPFLIPAGEWRYIEAGIIQRAQ
                     LLSLLLEDLYGPQELVTQGHFPAALLYANPAFLRPLVGVPVPAHSYLHMLAIDLARSP
                     DGQWWVLADRTQAPSGSGYALENRRIVSDVLPDLFRTSNVLRLASFFRAQRETLNSLA
                     QCDNPRIVLLTPGPYNETYFEHSYIARYLGFTLVGGADLTVRDRCVYLKTVDGLEQVD
                     VILRRVDDSFCDPLELRGDSLLGVPGLVDAIVAGNVKVANALGSGLIETAAVMPFLPG
                     LSKHLLDEKLKLPSVATWWCGQADALDWVLSHLDSVVVKPAFPSRGMEPVFGAELKPA
                     EKGRLADQLRARPHEYVAQEQIALSTAPVWDNGSLNSRSVVLRAYVLNTGSGWLAIPG
                     GLVRVADAEGSVVSMQRGGHSKDAWVLWDGPVDTFSMLRPRHEPVELHRASRAVPSSV
                     ADNAFWLGRYAERAENIARILRSVTPRVRRAEEAELGCLLRLHSCLESRHSKLSKAKH
                     RRPTSLELELELISLMTDKKRPDSLTSTLSEVSRIGGSVRERLSADMMFLIGQLRDSM
                     PTERITQFLESPAMLTGCLELLSAFSGMEHENINRGLGWLFLSLGRRFERAIYLTRQL
                     REVTRPLAEQDWPLLECLLEVADSSMIYRTRYFTTLQPLAVLDVIMADETNPRSLVFH
                     LSQIVDLYQKLPRHLPEDAQAMRNALVSLRNFDLRELKFPLPGAATVANGSDGLSRLE
                     RFLRELESLLPSWSNSISSRYFSHARTLPTTIGQ"
     gene            complement(14010..17234)
                     /locus_tag="VFC15_17945"
     CDS             complement(14010..17234)
                     /locus_tag="VFC15_17945"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZM12096.1"
                     /translation="MGIQVALNHRTQYRYDKAVSLGPQVVQLRPAPHCRTPILSYSLN
                     VIPAEHLLNWQLDPHYNHLARLLFPHKTNEFVVEVELVADLSPFNPFDFFLEPGVESY
                     PFAYAPGLAKDLEPYRSVEQAGPRLQAFLASLSNEKCGTIDFLVKLNHKVRDEVSYVT
                     RLDPGVQTCEQTLESRSGSCRDSAWLLVQILRHLGIAARFVSGYLIQLAPDETAPEDP
                     NSPQHDSADLHAWAEAYLPGAGWIGMDPTSGLFAGEGHIPLVCTPNASQAAPIGGTVE
                     PAKVDFYHSMSLRRLNDAPRTAKPFSEEDWARLEQVAHRVDADLKARDVRLTMGGEPT
                     FVGLDEPESPQWNIDALGPIKRVRGMALIQRLREKMAPGALLHFGQGKWYPGEPLPRW
                     ALGCYWRRDGVAVWENIDLVAPEDQDYRFGPSDALEFMEALTRRLQVSSENLLSAYSD
                     EVGTTQPAGYILPIRRRQPAGRLCWSSQLWFPRPERLLLTPGDSPIGYRIPTESMPWV
                     APDELVYECDKPPYAAQAKLPSRQSRMDLFQKIPAADVLPALSSKAETAPELIRPSLC
                     VQAREGRLHVFLPYASKLADYLDLLAAVEDTCQYLHKPVWLEGYPPPADPRLRSFSVT
                     PDPGVLEVNLPPASDWDELEQINTLLFEEARLNRLTADKFAYDGSHIATGGGSHIVLG
                     GATVLDSPLLRRPDLLRSMVAFWQNHPSLSYLFSGMYVGPTSQHPRVDEARMDVLYEL
                     EVAFSQLPANDCPPYVVDGLFRSLLVDVTGNSHRSEFCIDKLYPPEGLGLRLGLLELR
                     AFEMTPHVRMGLAEMLLIRALVSMFWKQPFTGSLVRWGTALHDRFMLPHFVRQDFSDV
                     LSLLRRSGYNFEEKWFASHLEFRFPKIGSIEVAAVALELRQALEPWNVLAEETTSGRT
                     VRSVDSSLQRMQVKVSGFTTESRYVVACNGRRVPLHPTGEIGEAVAGVRYRARRLSEE
                     LHPTIPVHFPLTFDIIDRWKERSIGRCIYRAGPPDGHLYTARPANAKEAEDRRRARFQ
                     TCGPVPGTMAEPQEETNSIFPMTLDLRLPMPKHKAGIEKPRLAP"
     gene            complement(18140..20422)
                     /locus_tag="VFC15_17950"
     CDS             complement(18140..20422)
                     /locus_tag="VFC15_17950"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM12097.1"
                     /translation="MRTAIENVSRRGFVKGMFSAGAFVLAVHYVPGMSFAEEQTARSV
                     IDHAPLHPSVYVGVESDGTVYIVAHRSEMGTSSRTSLPLILADELDADWNRVKIEQAI
                     GDPRYGSQDTDGSKSVREFFNPMREAGATTRLMLTRAAAQQWGVSPDECTTGLHQVIH
                     SASGRKADYGQLVTAAAKLPVPAKGEVRFKPRSQWRYIGKGENPYDLRDYCTGKANFG
                     IDAKTDGMLYAVIAHPPVLGGKVKILDDKQALQVKGVRQTITIETFKPPCHFQPLGGV
                     AVLADNTWAAMQGRKQLKIDWDDGPNASYNSEEFKKQLQETARKPGKVWRNQGDVDAE
                     FAKGGKIIEADYYLPHLAHASMEPPMALADVRGDRVEVWTCTQNPQAVQESIGPALGI
                     PKENVTCHVTLLGGGFGRKSKPDYAVEAAVLSKKTGKPVKVVWSREDDIRFDYYHAVS
                     AEHMKATVDAKGKPTAWLQRNAFPTIGSTFQAGAESPGAGELQQGWTDIPYDLPNLRV
                     ENGPAPAHVRIGWLRSVANIYQTFAVQTFTAELAANAGRDHIEFALDLIGPPRIINLK
                     AMGVDYPNYGASLDEYPVDTARLRHVIELVAEKSGWGKEKPGNGVGYGFAAHRSFLTY
                     VACVVKVQVNDDGDITIPRVDYVVDAGTVVNPDRTKSQFEGAAVFGTSVARYGEITAK
                     NGVIEQSNFNDYPVTRINEAPYQTNVYLVENDAPPAGVGEPGVPPFVPALCNAISAAT
                     GQRIRELPLSKLDFSKTAKS"
     gene            complement(20419..20877)
                     /locus_tag="VFC15_17955"
     CDS             complement(20419..20877)
                     /locus_tag="VFC15_17955"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM12098.1"
                     /translation="MIKFKLNGQERSFEGDPSMPLLWYVRDIAGLSGTKFGCGMGLCG
                     ACTVHRNGKAVRSCSTPMSGVSGQEILTIEAIAGPGGLHPVQKAWIENSVPQCGYCQS
                     GQIMQAVALLSETPKPSDTEIENAMAGNICRCGTYQRIRAAIKQAAGAKA"
BASE COUNT         4290 a         6231 c         6167 g         4336 t
ORIGIN      
        1 gatttccatt cccccagaaa cggccctgcg caggttcgag aaaacgccgc ttgccaaggc
       61 attcgtcgtg cagctcgtcc aacgtctacg cgacgtcaac ccaaacatgg gtgcggtgct
      121 gcgttggctc gaccagcgac tggccgagca agggaccacg gctgacgaaa ttgtccgcac
      181 cgagtaccag gaacaaaccg cgatgaacgt caccgtgcgg aacatcatcc ccagcatgcg
      241 cctgatgatg gactttgact ggaatgattt cgtcgagagc atcagcctgg tggacgaagt
      301 tttgcggagc gaaaccaact tcgatgaaat ggattttgcc acgcgcgata cgtaccggca
      361 tgcgatcgag gacctctcgc ggggctcaga ccacaaggaa atcgaggttg cggagcacgt
      421 cgtacaccgc atcaagcggg cccggactgc gccgcccgga gacggacaac ctcgcgggga
      481 ccgaagcacc gatcccggct attacctcat atccacggga cgtattgcgt ttgagcgcga
      541 actcggcttc cgcgtgccgt ggaaaaccaa ggtgctgcgt ttctatatgc gaagcgccac
      601 cttgagttac ctggggacga tcgcgctggg tacagcactg atcgtggcgc tgccgctgct
      661 ccacgaaaga aaagccggag tggcagggtg ggggctgtgg ttgctcggac tcctcgccgt
      721 ggtgcctgca tccgatctcg ccatcgcact catcaaccgg ttcgtaacag atttgctcgg
      781 cccacggatg ttgccgcggc tggagttgcg cgatggcgtg acgccgcagt tgcggaccat
      841 ggtcgtcgtc ccgaccctgc ttacgaacca gcatgacgtc gaacaactgg tggagcggct
      901 ggaagttcac tacctggcta attccgaggg tgatcttcgc tttgcgctgc tcaccgactg
      961 gatggacgcg gcgagcgaga ccatccccgc cgacaacgaa ctgctggcgc tcgccgtgga
     1021 tggggttgcc cggctgaaca aaatacatgg tcccactccc gacggcgacg cgcgcttcca
     1081 gctgctgcat agaaagcgcg tctggaacga gagcgaggcc aaatggatgg gatgggaacg
     1141 caagcgagga aagctggttg agctgaacca gcttctccgc ggcggtatga atactacgtt
     1201 tcttccggtc ggcggacgcc cggcgcaagc tcccgcggga gtccggtatg tcatcacact
     1261 ggatgcggac acgcggatgc cgcgaggcgt agcctgccgt ctggtcggca ccatggcgca
     1321 ccctctcaat cagccaaggt tcagcgcagg ccacgggcga gttgtggagg gctatggaat
     1381 tgttcagccg agaatcacac attcgctgac agccgatcac gagagatcat ttttccaggc
     1441 gctttttgcc ggtcccagcg gcatcgatcc gtacgcttcg gcggtttcgg atgtgtacca
     1501 ggacttgttc gaagaaggtt cgttcaccgg aaaaggaatc tacgacgtcg atgcggttga
     1561 aaaggccatg gatggcaagg tgccggaaaa cgcgttgttg agccacgatc tctttgaagg
     1621 ggaattcgcg cgcacggcac tggcttcaga cattgagctt ttcgaagagt atccgtccca
     1681 ctatgaggtg gcggctgccc ggcaacaccg ttgggtacgg ggagactggc aacttctacc
     1741 ctggatcatg ggtcgcccgc gggccaggtc tgaaggtcac cccaaggtcg ccattccggc
     1801 gattggccgg tggaagatgt tggacaatct gcgccgctcg ctctctgcac cggctgcgtt
     1861 ttttgcgctg atcgcggcct gcctgatccc tgcagcttct tcgtgggtgt gggcgagatt
     1921 cattcttgcc acgatggcga tccccgctct cctctctttc ctgatcggca tcaatccgcc
     1981 gcgtcgaggc atttccaagc gcagtcactt ccggtcaatg ctgagcgact tatccctcgg
     2041 tgcctcccag ttaggcgtga ccatcacgat gctggcctac caagcctggc tgatgctgga
     2101 tgcaatcatc cgcacactcg ttcgcatgac tgtgacacat cggcacctgc tggaatgggt
     2161 gaccgcggcc cgagcgaaac acgctgtcga tttgacgatc accggcgtct atcggcgcat
     2221 ggcggggggc gtgctgttcg cgatggcggc gttggtgatc gtcgcctcct ggggtcatcg
     2281 cggttggggc gtcctgctcc cgttcattgc tctctggctt gccgctcctg cggtggcatg
     2341 gcagatcagt cttcctcccc ggcgcagcgg tgccgagccg ctctcggcgg cgaacgcccg
     2401 cgccctgcac ttgattacgc gccgaacctg gcgtttcttc gagattttca tcgctccggc
     2461 cgaccatgct ttgcctcccg acaactttca ggaagaaccg acgccggtta tcgcccatcg
     2521 aacgtcgcca acgaatattg ggttgtatct gctgtccacg caggcggcgc acgatctggg
     2581 gtacctgggc acgctggaca cagtcgaccg actggaagcc acgctcggga ccctgagcca
     2641 attggaatta gtgcgagggc atttctacaa ctggtacgac actcgcgatc ttcggcccct
     2701 ggatcccaag tatgtctcgt ccgtagacag tggaaacctc gccggacatt tgctggccct
     2761 ggctaacggc tgccgtgaac tcatccagaa atcctcactg ggaacgagca cgcttggcgg
     2821 cgtgcaggac acgatcgagc tcctgcggga agccgtgagc cagatcgcag aaacgcggcg
     2881 gacgaacacg gttaccagga aacagctcag caatgccatc gacgcactgg ccaactcgct
     2941 ggaacttgcg cccattaatc cggcggagtg ggcgatacgt ctcgcagaac tgaggacgcg
     3001 ggctgtgacc gtcgctgaca ttgctcagac catggcccag gaacgcggcg atgcatcgga
     3061 ttccgaattg ggtgtctggg cggatgcagt taaggcttgc gtcgaaagtc atatcagaga
     3121 cgcggaaatt ctgcttccct ggttgcgccg cgattcggtc agcaatcccg ccataacacg
     3181 acgttcacag gagcaggcgc ccgagtgggt agccattgaa ccgttctttg ggtcggtacc
     3241 taagctggcg gacattcccg aacgaaccga agaagcccag cgagcactgg ccttattgcg
     3301 aacgagcctg gtcaacgatt cgtcggcgaa cttcgagata ctggcgcgcg ttgatgcctt
     3361 gacccaatcc ctacggcagt cggggaagga tgctatcgcg ctcgctcgcc gtctgtccgc
     3421 catcgcgcaa aaggccgacg acatgttcca ggcgatggac ttcagctttc tttttgatcc
     3481 cacgcgcaag cagttttcca tcggataccg tgtcgccgaa aacaagctgg acgccaactg
     3541 ttacgacctg ctggcttcgg aagcgcgcct taccagcttt atcgcgatcg cgaaaggcga
     3601 tgttccctcc tcccactggt tccgcctggg gcgggcactc acgcccgtgg gccgcggctc
     3661 agccctgatc tcgtggtcag ggtctatgtt cgaatacctg atgcccgcgc tgatgatggt
     3721 ttcgccgaca ggcagtttgc tgaaccagac gtacgaactg gtggtgcgcc ggcagattgc
     3781 ttacggcgcg gagcgtggcg tcccctgggg aatttcggag tcggcctaca acgcgaggga
     3841 cttggatttc aattatcaat acacggcgtt tggcgttccg ggattggggc taaaacgcgg
     3901 actcagtgaa gatctggtga ttgcaccgta cgctaccgca ctggcggcga tgattgaccc
     3961 caatgcggcg gcggagaact ttgcgcaact cgctaccgcc ggcggcaaag gcgtttacgg
     4021 gttctacgaa gccctcgatt acacgggtga gcgactgccc gaaggagaaa atgttgcggt
     4081 ggtgcgggct tacatggcgc accatcaggg aatgtcgctg gttgcactcg ccaacgtcct
     4141 gagcgacggc gcgatgcggg accgcttcca cgccgagccg atggtgcagg cctctgaact
     4201 gctgctacag gaacggacgc cgcgtgacgt gctggtggcg cgtccgcgcg ccgaagaagt
     4261 ctctgcggca gcgcacgtcc gcgaaatggt tccgccagta gtgcgccgct tcagctcacc
     4321 tcatgaattg atcccgcgca cacatctgct ttccaacggg cgctacgggg tgatggtgac
     4381 aacggccgga tcgggctata gccgatggca gaatctcgcc gtctctcgct ggcgcgaaga
     4441 cgttacccgg gactgctggg gatcctacat ctttcttcgt gatgggcaga gcggccagat
     4501 ttggtcggct ggatatcagc cctcaggtgt cgaaccggac agctacgaag ccagcttcgc
     4561 cgaagatcga gccgagttca ttcgacgcga cgggtcgctg gtcaccacgc tggaggtggt
     4621 ggtttcgcca gaagacgatg cggaagttcg ccgggtttcc atcgctaacc tgggaacccg
     4681 gacccgtgaa gtccaggtca cgtcctacgc cgaggtcgtg ctcgctcctc aggctgccga
     4741 tgaagcacac ccggcgttct ccaatctgtt tgtggaaaca gagttcgtcg ccagcgcgga
     4801 agtattgctg gcgacacgcc ggcggcagtc cgaaagagac acgccggcct ggctcgctca
     4861 cgttgcgatg atcgaaggcg agtcagttgg agaattgcaa ttcgagacgg atcgcgcccg
     4921 cttcctggga cggggccact cggtacgcga ggcaatatct atcgcggatg gtcgccctct
     4981 ttccaatact gccggcccgg tgctcgaccc cgtgatgagt ctgagacgga ccgttcgcat
     5041 cccgccgggt gggaccgcgc ggattgcatt ctcgaccctc gtcgccgcgg cgcgcgagca
     5101 ggtgctcgat ctggcggaca aatacaggga tgcgcgaact tgggagcgga cggtgatggc
     5161 ggcatggacg caggcgcaag tgcaacttcg gcatcttggc atcacccctg acgaagccca
     5221 cttgttccag cgactggcca atggcatcgt gtactccgat ccatccctgc gaccgtctgc
     5281 cgacatgctg agccgcacca cgattgacct ccctgcccta tgggcgcacg gaatttccgg
     5341 cgatctgccc atcgccctgg taaggataga cgaggccgag gatgttggga tcatccggca
     5401 attactgagg gcgcaggaat atttgctcac gaaggggttg tcggttgaca tcgtgattct
     5461 caacgagaag ccgccttcct atgaacagga actccagggt tcgttggacg atctggtacg
     5521 tgcccaccgg cagcgagtgt cgtcggaaca cggcgaggtc cacggcaacg tctttctgct
     5581 tcgcggagat ttgatttcag tgcaggagcg cgcgctgttg caatccgcag cccgcgtagt
     5641 gctgctcagc cgccacggga cattagccga gcaggttatc cggtcgcaac gctcggagac
     5701 tctaacgcga ccggcgcctc gtccgcctcg attgggaaaa gacgcagaag tgccgttgga
     5761 cgcgccggat cttgaattct tcaatggctt aggcggcttc gccgaaggcg gaagtgaata
     5821 tgtgaccgtc cttggggaag gattgcgcac cccagctccg tggatcaacg tgatcgcaaa
     5881 tccgtcgttc ggttttctgg tttccgagtc tggctccggg tatacctggt cgggcaatag
     5941 tcatgaaaat caactcactc cctggtcgaa cgatcccgtg accgacccgc ccggtgaagc
     6001 catctatctt cgcgatgagg agaccggtgc ggtatggagc ccgacggcgc taccggtccg
     6061 cgatgaggca tccggctatg cggcatggca tggccagggc tttagccggt tccgcaatgg
     6121 atcgcagggt atcgtttcag agctgctgca gtacgttccg attcgcgatt cgatcaagat
     6181 ctcgcggttg gttctgcaaa accgttcggc ccgcactcgc cgtctttcgg tgactgcttt
     6241 tgcggaatgg gtgttgggga gttcgcgggg cattactgct ccgcacatta tttcggaact
     6301 cgattcggaa acgaacgctc tacttgcccg cagcggatgg ggcggcgagt ttggagggcg
     6361 aattgctttc cttgacctct gtggacggca gacctcatgg tcgggcgacc gcaccgagtt
     6421 cctgggacgt aacggagtga ctgcgcggcc tgcggcactg caaaggggcg ggccgctttc
     6481 cggaaaggtt ggtgccagtc tcgatccatg cgccgcgctg cagacggtgg tggagttgcg
     6541 tcccgatgaa cgcgtcgaga ttgtagtttt gctgggccag gggaaagacc ggcagcaagc
     6601 gcgcgaattg atcaattgct atcgcgcggc gaacctcgac catgtcctgg ccgaggtcac
     6661 ccggcaatgg gacgaagtcc tcggctcggt gcagatcaca actcctgacc ggtcgatgga
     6721 tattctggtg aaccgctggc tgctctacca gacgctttcc tgccgggtat ggggacgcgc
     6781 cgcgttttat cagaccagtg gggcttacgg tttccgcgat caactgcagg acgtgatggc
     6841 gctcaccgtg gctaaacgcg atgtcgcgca tgcgcatctg ttgcgtgccg cctcacgcca
     6901 attcatccag ggcgacgtcc agcactggtg gcatccaccg tcgggacgcg gcgtcagaac
     6961 tcgcatttcc gatgacttgc tctggctgcc ttatgccgta atccattaca tcgaggttac
     7021 gggagagtgg ggcctgcttg acgaggcggt gccattcctg gaaggcgatt tgctcgcgga
     7081 gggacaactg gaatcctact ttcagccacg ggtctccgag acgcgggctt ccctctttga
     7141 gcattgcgcc cgggcgctcg accggagcct cgccgtcggt atccacgggc ttccgctgat
     7201 cggcacggga gactggaacg acggaatgaa ccgggttgga caagatggta agggtgagag
     7261 tgtttggctg ggctggttcc tgcacactat tctctgggaa ttcgccaagg ttgcggatgc
     7321 gcgtggcgaa cgcaagcgcg ctgaaacctg gcggctgcat gtcagcgcgc tcaaggccgc
     7381 tctcgagcgc gatggctggg acggtgagtg gtatcgccgg gccttcttcg atgatggcac
     7441 tccgctcggc accgcccaag acaaggagtg ccgcattgac tcgattgtgc agtcctgggg
     7501 agtcctaagt ggcaccgctg aaccagcccg tgcggcgcga gccatggccg cagtggagaa
     7561 atatctggtg cggcgtccgg agggcctggt tttggtactg actccgcctt tcgataagac
     7621 ggaccacgac cctggctaca ttaagggcta tctgccggga atccgcgaaa atggcggcca
     7681 gtatacccac gctgccgcat ggacggtcct ggcatctgcg gcactcagcg acggagataa
     7741 ggccgtcgaa ctgtttcgca tgctcaaccc gatcaaccgc acctcgtcgc gagcgactgt
     7801 gcagcgatac aaggtggagc cttacgtagc ggcaggcgac gtctattccg aatctccgca
     7861 cgtcgggcgc ggcggatgga cttggtacac cggttcggcc ggatggctct accgtggcgc
     7921 catcgaatgg cttctcggct tcagactgcg gggaacagtg ctctcgatcg atccctgcat
     7981 cccgcgaaac tggccgagct actccatccg ttttcgctat cactcgacca tatacaacat
     8041 caaggtggaa aatccgtcgg gcgtttcacg gggtgtcgcc ctggctgaaa tggacggcgc
     8101 tcgcctgctc aactccccca atgttccgct tgtcgatgat gccaaggagc accaggtgcg
     8161 cattgttatg gggtagggga tgatctcgaa gtggagaagg agacctatta atcgcctacg
     8221 gaataccgag atcgtcttcg cttcagttgc gtgggatgga ttaaatggaa tcgcccgcta
     8281 attcgggcgc gactgcaaga gcgcttcccc gtggctcaga tcgaggtcct tcgacacgga
     8341 ttcaaaatca gaccgtgtct catgttgctg cttactcacc gtcttgaaca acagcattcg
     8401 cccgtccacg tgaatatcga cgagttgggt cttccaatga gaggggctca cctggattcg
     8461 ctcaatctca aacgtaccgc ctttggcgat tcgcccgaga atcccgaaac caaaatcgac
     8521 gttctccacc aaacgcccgt tcatgcgcgc cagtcgtttc tgctggggac tgactaaaat
     8581 cgttcccgcc atgctctgaa gcgcttttga ttccatcgtc agagggacaa atgccgggtc
     8641 aggccgaaag ctcagtcgca gatccgggcc atcccaaccg tcgtattgga acaggaacgc
     8701 gctgggcaac agacgcacaa agttttccag ccggagttcg tccgcatcgt tctgcttctt
     8761 gaccgcaagt tgctggctcg gctcacgaac gagttgctcc tgccgtgtcg tctcccgttg
     8821 ttgctgggcc gaatctaagg gtgcgtcatt aatcgaaagg agccgatgca ctgggccgtc
     8881 cttcgtctca acttcaagct gggtcagtgt ctgttgctcg actactttgg tgacgcggta
     8941 catccagttg ctgtgttccg tgcgatccgt caattcattc gcaacaacat ccttcataag
     9001 atcttgtgcc gaagagccca aatctggcgc aggggggccg gagtagcacg ggcatccgag
     9061 gagcagcacc gctgcgagat gaaagacgag atacttcaca gaatgttgga tgcatgatag
     9121 ctagacgcag gcagagttta tttccattcc accattcgcg caatgtgagc agatgtgtac
     9181 atacgtagaa cgccgtattg cttactcttg ttgtgtagtg gcataagtgt atagcgctat
     9241 tcgattagtg ccgccgagta ttccgtcgtg cgcgatgagc aaatgtaacg atgatccgga
     9301 atggaagttg agctaacttt gagaaacgcc atggcaagta actgccgaac gcacatgatt
     9361 cgaatgctgg cgttgttgct ggtaacgctg atgttcagcg gccatgggac agcttattcc
     9421 gtgctgactc acgaagagat cgttgatttg ctgtgggcag accagatcag gccgcttatc
     9481 ctcaagcgct accctggact ctccgaagag cagatcaagg aagcccatgc ctacgcatac
     9541 ggcggggccg tcatacaaga cctcgggtat tatccattcg gcagcgtcga gttcagcgac
     9601 ctggtgcact acgttcgcac cggagacttc gttcgtgaac tgatagcgca aagcaacgac
     9661 gcaaatgagt acgcgttttc tctgggcgcg ctgtcgcatt atgcatcgga tatcgccggc
     9721 cacccggcgg tcaatcaggc cgtcgccatc gagtatccca agctgcgagc aaagtacggc
     9781 gcttcagtcc gctacgcgca ggacaaaacc gcgcatctga aaacggagtt cggcttcgac
     9841 accgtgcagg tggcgaaaaa tcgttatgct tcgcagcaat accacgattt catcggattc
     9901 caggtgtcca agcccctgtt ggagcgggtg ttccccatcg tctacgggct ggagttaaaa
     9961 gacgttctgc ctcgcgaaga ccttgccatc ggctcgtatc gctactctgt cagctcattg
    10021 attccagaaa tgacccaggt tgcgctgcaa actcataaga aagaattcat gagcgagacc
    10081 cccgatttcg caaagcagaa gtttctgtac cgcatttccc gctcggaata tgaaaaggaa
    10141 tggggaaaag attacgtcaa gccgggcttg ggcacgcgca tcttgtcaac tctcctgcga
    10201 tatatgccga aaatcggtcc cttcaaaaag ctggcgttca atagcccgac tactcaaacc
    10261 gaagatatgt acatcaagag catcaatacg acggttgaac aataccgggc cctcctcata
    10321 gcagagcgtg cggacaagct cgtgctcccc aactacgact ttgacagcgg taatccgact
    10381 aagatagccg aatactcgct tgcggatgac acttacgaga aactgctggg tcaattggct
    10441 aagagcaagt tcgaccgtac gacgcccgag ctgcgcgaca atgtcttact gttctattcc
    10501 gacttcgcgg catccaacga gagcaaagac gacaaggttt catcggctaa tttgatggag
    10561 tggcttggtc agttaagagc cgcgaaactg gttccggcta ctgaagctgt ggccagccgc
    10621 taatcggtca ggccaacaat taacaatcca gcggctccat gtcaacggcg actttaagta
    10681 ggtgtgatcc gccgcccaga atcaagccat gcagaggact gacgtcgccg tagtcccgcc
    10741 cccaggccag cgttacgtgg ccgtctgagg gaacaaggtt gttagtcggg tccatgtcaa
    10801 gccagcctat tcccggacaa taggcggaca cccatgcatg ggatgcgtcg gcacccacca
    10861 gcctgggttg cccgggaggc ggataggttc gaaggtatcc gctgacataa cgggctgcga
    10921 gattcaggga gcggagacag gcaatttgca ggtgtgcgaa atcctggcac acgccacgcc
    10981 gcttgcgaaa tacttcttcg gtggacgtgc ggacgttggt cacctttgca tcgaagcgga
    11041 agtccctgta aatgcgcgcg gtcagatccc gaagcgcctc cggcatagga cgccggggcg
    11101 tgaatgactg aagcgcgtat gcggcatact cgggccgtat ccggatacgc ggcgactcaa
    11161 atccaaactg ataggcttcc agccctgccg ggctgtgatc gttgggaagc aatcttgcgg
    11221 cctcttccca agggatcgac ggttgcaaca accgccttcc gttcccgccg attgtcactt
    11281 cgcttcgcgc ctcgacgact aattccttgt gtggctcttg aatagtgaaa taacagagaa
    11341 gattcccaaa gtagtccaac cgttccgtaa gtgttgccgg tgttggttgg atgcacaatt
    11401 cggtctgcgc caactggtga tgcaacgaag accgcggctt caggcacgcc acgtggtatc
    11461 cggctgagac cggatacttg tacttataag tggtgcgatg aacaattcga tagatcattg
    11521 acctatggtg gtgggcaacg tgcgagcgtg gctaaaatac ctgctcgata tgctattcga
    11581 ccaggaaggc aacaaacttt ccagttctct gaggaaccgc tcaagccggg agagcccatc
    11641 ggaaccattt gccaccgtcg cagcgcctgg caatggaaac tttaactccc gcaaatcaaa
    11701 attgcgcaag gataccagcg cgttccgcat tgcctgcgcg tcctctggca ggtgtcgagg
    11761 aagcttctga taaagatcca cgatttggct cagatgaaag actaacgacc gtggattggt
    11821 ttcatccgcc attatcacat ctagtacggc taagggctgc agggtcgtga agtaccgtgt
    11881 ccggtagatc atggagctgt cggcgacctc gagcaagcat tccagaagcg gccagtcctg
    11941 ttcagcgagc ggcctggtaa cctcgcgcag ttgcctggtc agataaatcg cgcgctcgaa
    12001 acgacgtccg aggctgagga acaaccagcc cagtccacgg ttgatgttct cgtgctccat
    12061 cccggaaaat gccgaaagca gttccaagca cccggtcagc atcgctggag actcgaggaa
    12121 ctgtgtgata cgctcggtcg gcattgagtc ccgtagctgg ccgatgagga acatcatatc
    12181 ggccgacaat cgttcgcgaa cgcttccgcc aatccgggac acctctgaca aagttgacgt
    12241 caggctgtct ggccgctttt tatcggtcat cagggaaatc aattccagtt ccagttcgag
    12301 cgaggtcggc cgcctgtgct tcgcctttga aagcttgctg tgccgtgact ccaagcaact
    12361 gtgcagccgg agaagacagc caagttcggc ttcttcagcg cgccgaacgc gcggagtaac
    12421 tgagcgcagg atccgagcga tgttttcggc ccgttcggcg tatcgaccga gccagaaggc
    12481 attgtctgcc acactgctgg gaacagcccg cgaggcgcgg tgcaattcga cgggttcatg
    12541 gcgtgggcgc agcatgctga acgtatcgac ggggccgtcc cagaggaccc atgcgtcctt
    12601 gctgtggccg ccacgctgca tcgagacaac cgatccttcc gcatcggcga cacgtaccaa
    12661 ccctcccggg atggcgagcc acccgctgcc ggtgttgaga acgtaggcgc gcaacaccac
    12721 gctgcgcgag ttgaggctac cgttgtccca caccggcgct gtggataaag caatctgttc
    12781 ctgcgccacg tactcatgtg ggcgggcccg caattgatcg gccagccttc ccttttcggc
    12841 cggcttgagt tcggccccaa agaccggctc cataccgcgt gagggaaacg ccggttttac
    12901 cactacggaa tcgagatggc tgagcaccca atccagcgcg tcggcctgcc cgcaccacca
    12961 agtcgcaacc gatggaagct tcagtttctc gtcgagcaga tgcttgctaa ggccgggaag
    13021 aaaaggcatg accgcggctg tctcaatcag accgcttcct aatgcattcg cgaccttcac
    13081 gttgccagcc acgatggcat cgaccaggcc gggaacaccc agaagcgaat cgccgcgcaa
    13141 ttcgagaggg tcgcaaaagc tatcgtccac ccggcgcaaa atgacatcga cctgctccaa
    13201 cccgtcgacc gtctttagat agacgcatcg atcgcgcact gtgaggtccg ctcctccgac
    13261 cagagtgaat cccaggtatc gagcgatgta cgagtgctcg aaatatgttt cgttatacgg
    13321 tccgggggta agcagtacga ttcgcggatt atcgcactgc gcgagactgt tgagcgtttc
    13381 gcgctgcgcc cggaagaaag aagcgagccg caaaacgttc gacgtccgaa agagatccgg
    13441 cagaacatcg gagacaatcc tgcggttctc cagtgcatag ccgctgcccg aaggtgcctg
    13501 ggtgcgatcg gccagcaccc accactggcc atcgggcgag cgcgccaggt caatggcgag
    13561 catatgcaga taactgtgag cgggaacagg tacgcccacc agcggccgaa ggaacgcagg
    13621 gtttgcgtaa agcagtgccg cgggaaagtg gccctgcgtc accagttcct gtggtccgta
    13681 gaggtcctcg agcagcagac tcagcagttg ggcgcgctga atgatgcccg cttcgatgta
    13741 gcgccactcc cccgccggaa ttaagaatgg gacgatatcg atcgtccaag gtcgatttac
    13801 cccgagagga tcgctgtaaa cgttgtaggt tatcccattc tcgcgaatgc ggcgctcggc
    13861 acggccccag cgtcgtccca actcctcgcg tccgatcgcc cgcagcgact ccatcagatg
    13921 cgcccagtgc ggccgaggcg tcacgccatc ggcagacaat tcgtcatagg aatctccgta
    13981 gcggagtgga ctttgatata agtcccaagt catggggcga gcctcggctt ctcgatgccg
    14041 gccttatgtt tgggcatcgg caaccgcaaa tccagagtca tagggaagat cgagttcgtc
    14101 tcttcctggg gttccgccat ggtgccgggc actgggccgc atgtctggaa gcgtgcccta
    14161 cgccggtctt cggcctcctt agcgttggct ggtcgggctg tgtacaagtg accgtcaggc
    14221 ggtcccgcgc ggtagatgca tcggccaatc gaacgctctt tccatcggtc gataatgtcg
    14281 aaggtcaggg gaaagtgcac cgggatggtc ggatgcaact cctccgatag ccgtcgggca
    14341 cgatagcgaa cgccagctac cgcctctcca atctcgccgg tcgggtggag cggaaccctg
    14401 cgcccgttgc atgcaaccac gtaacgggat tccgtcgtga acccggagac cttcacctgc
    14461 atccgctgga gggacgagtc aacgctgcga accgttctgc cggaagtagt ctcttcggcg
    14521 agcacgttcc aaggctcgag ggcttgacgc aactcgagtg cgactgccgc cacctcgatc
    14581 gacccgatct tcggaaagcg aaattccaag tgcgaagcaa accatttctc ctcgaagttg
    14641 tacccggacc gccgcagaag gctaagcaca tcggaaaagt cttgcctgac aaaatgcggc
    14701 agcataaaac gatcatgcag cgcggtgccc catcgaacca ggctgcctgt gaacggctgt
    14761 ttccaaaaca tcgagaccag agcacggatt agaagcatct cggcaagacc cattctgaca
    14821 tggggagtca tttcgaaggc gcgcaattcg agcaagccga ggcgcaaccc caaaccctct
    14881 ggcggataga gtttgtcgat acagaattcg gagcggtgcg aatttcctgt gacatcgacg
    14941 agcaggctgc ggaacagtcc atccacaacg tacggggggc agtcgttcgc gggcaactgg
    15001 ctgaaggcca cctccagctc atacaaaacg tccattcgtg cttcgtccac acgcggatgc
    15061 tggctcgtcg gaccgacata cattccggaa aacaaatacg acagcgaggg atggttctgc
    15121 cagaaggcca ccatgctccg caggagatct gggcggcgca ggagcgggct gtccaacacg
    15181 gtcgctccgc cgagcacgat atggcttccg cctccggttg cgatgtggct gccgtcgtac
    15241 gcgaatttat cagccgtcaa tcggttaagg cgtgcttcct cgaagagcaa cgtgttgatc
    15301 tgttcgagtt cgtcccaatc gctcgcaggc ggcagattca cctccagaac tccgggatcg
    15361 ggagtgacgc tgaacgatcg caaccgcggg tcggcaggtg gagggtagcc ctccagccac
    15421 accggcttgt gcagatactg gcaagtatcc tcaaccgctg cgagcaggtc gagatagtcc
    15481 gcaagtttgg acgcataggg tagaaacaca tgcaggcggc cttcgcgggc ctgcacgcac
    15541 agcgatggcc gtatcagctc cggcgcggtt tcagccttgc ttgagagagc gggcagcacg
    15601 tcggccgctg gaattttctg gaacaaatcc atgcggctct gccgtgacgg gagcttggcc
    15661 tgagccgcgt agggaggttt atcgcactcg tatacaagtt cgtccggcgc tacccagggc
    15721 atcgactcgg tcggtatgcg atagcctatg ggcgaatccc ctggcgttag cagcagacgt
    15781 tccgggcgcg ggaaccacaa ctggctcgac cagcataatc ggcccgccgg ttgtcggcgg
    15841 cgaatgggca aaatgtaccc cgccggttgc gtcgttccga cttcgtcgct gtaggcggaa
    15901 agcagatttt ctgagctgac ctgaagtcgg cgcgttagcg cttccatgaa ctcgagagca
    15961 tcggaaggtc cgaaccgata gtcctgatct tcgggggcga cgagatcgat gttctcccag
    16021 acagcaacgc cgtcgcgacg ccaataacag cccagcgccc aacgcggcaa cggttcgccg
    16081 ggataccatt tgccctgtcc gaagtgaagc agtgcaccgg gcgccatctt ctcgcgaagc
    16141 cgttgaatga gagccatccc gcggaccctc tttattggtc ccaaagcgtc gatattccat
    16201 tgcgggctct cgggctcatc gaggccgacg aaggtaggct cgcctcccat ggtgagtcgc
    16261 acatcccgcg cctttaagtc ggcgtcaacg cgatgcgcca cctgttcgag ccgcgcccaa
    16321 tcttcttcgc tgaagggttt ggccgttcgt ggggcgtcat tcaggcgacg aagggacatg
    16381 gagtggtaga agtccacctt agccggctct accgtgcccc ctatgggcgc tgcctgagaa
    16441 gcattcgggg tgcagaccag cggaatgtgt ccctcgccgg cgaacaggcc cgaggttggg
    16501 tccatcccaa tccagcccgc accgggcaaa tacgcttctg cccaggcgtg cagatctgcc
    16561 gagtcgtgtt gcggactgtt cggatcctct ggcgcggtct cgtccggcgc caactgaata
    16621 agatatccgg agacaaagcg tgccgcgatc cccaaatgcc gcagaatctg caccagcagc
    16681 cacgcagaat cgcggcagga gcccgagcga ctttcaagtg tctgctcgca tgtctggaca
    16741 cccggatcca gccgcgttac ataagaaact tcgtcccgca ccttgtgatt caatttgacc
    16801 aaaaagtcga tcgttccgca cttctcgttc gaaagggacg caagaaaagc ctgcagccgt
    16861 ggaccggcct gttcgaccga gcggtaaggt tcgaggtcct tagccaaccc cggcgcatat
    16921 gcgaatgggt agctttcaac tcccggttcc aagaagaaat caaacggatt gaacggggag
    16981 aggtccgcga cgagctccac ttcaaccaca aactcgtttg ttttgtgcgg aaaaagcagg
    17041 cgcgccagat gattgtaatg gggatcgagc tgccaattga gaaggtgctc ggccggaatg
    17101 acgttaagtg agtagctgag tatcggcgtt cggcaatgcg gggcaggacg caactggaca
    17161 acctgtggac cgagtgacac cgccttgtcg taccggtact gcgtccggtg attgagagcg
    17221 acctgtatgc ccatccatcc tcatgcggcc aacaccttga gttcccggct ctttttcatt
    17281 aagcgcaact agccggtgaa ggcgcgaaac atcttgcgaa ggctgctgct ataaaactac
    17341 cttaccaacg gtgctagcgc tgcaaatttc tgagcagagc gccggacttc accgttccaa
    17401 ccttcacctc ttcgggcatc agtcacatct tcgggtgatg gcgcgcaccg acgaacccac
    17461 ccgaggcccc aatatcatag cttgggggct gcgctgtcca cataacggag gtcgcgatgg
    17521 cattatttcc tgtcctactc tgccggagtt gttctcggcc catcgcactc ccgcctgcca
    17581 gccaaccctg tacatctccg ccccaaccag cgtggcccgc agattgcgct ccacgaaact
    17641 tcctatgtcg tgagtgcaaa aacgtttacg agtattcagc gcacgatgtc tgtcaggtcc
    17701 cactggctga agcgggcgaa gatcaaggcc ataaacccct gaatgtcgtt tgcatcgaaa
    17761 taccatgcag cgggtcgggt tgttcggcgc tgatgagagt ccacaccctc atggcgttcg
    17821 acgccgacct gaaagcagaa accccggaag tcctcgcgtc gtcgcacggt catcaagttc
    17881 gttgtgacag gggatacatt ttgagtaggc cgatccgtcc atccgagacc tcattctctg
    17941 cccacttcga caagcagtgg aagcgtccgg ggatttagtt aatattcttc ctgttcaagc
    18001 tgggtcaaga attgactttg aaagtcgctt cgggcaaccc cgacctaccg gcgtgtgtcg
    18061 acacgcaccc ggcctcggca tcgagcggac ctttttccca agtttctcgt cgccccaagc
    18121 gacgtgagta tatggaagat caactcttcg cggtcttgct gaaatcaagt ttcgacagcg
    18181 gcaactcccg aattcgctgg ccagtagcgg cggaaatcgc gttgcagaga gcgggcacaa
    18241 acggtggcac gcccggctcg ccaaccccgg ctggcggagc gtcattttcc accaagtaga
    18301 cattggtttg atagggagct tcgttgatgc gggtaaccgg atagtcatta aagttggatt
    18361 gctcgatcac tccattcttc gccgtgatct cgccataccg cgcgacgctg gtgccaaata
    18421 ccgccgcgcc ttcgaactgc gacttggtgc ggtcaggatt gacaaccgtc cccgcgtcaa
    18481 ccacgtaatc cacgcgcgga atcgtaatat ctccatcgtc gttcacttga accttaacca
    18541 cgcaggcgac gtaggttaga aaactgcggt gggcagcgaa tccgtatccc accccgttgc
    18601 ccggcttttc cttcccccat ccggacttct ccgccaccag ttcgattacg tgccgcagac
    18661 gcgcggtgtc gacgggatac tcgtccagcg acgcgccata attcggataa tccacaccca
    18721 tcgccttcag gttgatgatg cgcggcggcc caatgagatc aagtgcaaac tcgatgtggt
    18781 cacgccccgc attcgcagcc aactcggcgg taaacgtttg cactgcgaac gtctgataga
    18841 tgttggccac cgatcgcaac cagccaatgc gaacgtgcgc cggggccggt ccattctcga
    18901 cgcgtaggtt ggggagatca tacggaatgt cggtccatcc ctgctgcaat tcgcccgcgc
    18961 cgggggactc agctcccgcc tgaaacgtcg aaccgatggt aggaaacgca ttccgctgca
    19021 gccatgctgt cggctttccc ttcgcatcca ccgtcgcttt catgtgttcc gctgacaccg
    19081 cgtggtagta gtcgaaacga atgtcgtctt ccctgctcca taccaccttc accggcttgc
    19141 ctgtcttctt cgacagcact gcagcttcga cggcatagtc gggcttcgac ttccggccaa
    19201 acccgccgcc cagcaacgtc acgtggcacg tgacattctc cttggggatt cccagcgcgg
    19261 gtccaatcga ctcttgcact gcctgcggat tttgcgtaca agtccatacc tcgactcgat
    19321 ctccgcgcac atcggccagc gccatcggag gctccatcga agcgtgcgcc aggtgcggaa
    19381 ggtagtaatc cgcctcgatg atcttgcctc ccttggcgaa ttccgcatcg acgtcgccct
    19441 ggttgcgcca cacctttccc ggcttgcgcg cggtctcctg caactgcttc ttgaattcct
    19501 cggagttgta gcttgcgttg ggaccgtcgt cccaatcgat ctttagctgc ttgcggccct
    19561 gcatcgccgc ccaggtgttg tcggccagca ccgcaacccc acccagcggc tggaagtggc
    19621 acggcggctt gaaggtctca atggtgatcg tttgccgcac acccttgacc tgcaacgcct
    19681 gcttgtcatc aaggatcttg accttcccgc ccaacaccgg aggatgcgcg atcacggcat
    19741 agagcatgcc atcggttttc gcatcgatac caaagttggc tttgccggtg cagtagtcgc
    19801 gcagatcgta tgggttttct ccttttccga tgtagcgcca ttgcgagcgc ggcttgaatc
    19861 tcacttcgcc cttcgccggt accggaagct tcgcagccgc tgtgacaagt tgcccatagt
    19921 ctgccttgcg accgctagcg ctgtgaatca cctggtgcag accagtagtg cattcgtccg
    19981 gcgaaacgcc ccattgctgc gccgccgcgc gtgtcagcat caggcgggta gtcgctccgg
    20041 cttcgcgcat ggggttgaag aactcacgca ccgacttgga tccgtctgta tcctgcgatc
    20101 catagcgcgg atcgccgatc gcctgctcga tctttaccct gttccagtcg gcgtcgagtt
    20161 cgtccgcgag aatcagcggc agcgaggtgc ggctgctggt gcccatctcc gaacgatggg
    20221 cgacgatgta gaccgttccg tcgctctcca cgccaacgta aacactcgga tgaagcggtg
    20281 cgtgatcaat cacggaacgc gcagtttgtt cctcagcgaa actcattccg gggacgtagt
    20341 gcactgccag cacaaaggcg ccagcggaga acatgccttt cacaaatccg cgccggctga
    20401 cgttctcgat ggctgttctc atgccttcgc ccccgcagcc tgcttgatgg cggcccgaat
    20461 gcgctgatag gtcccgcacc ggcagatgtt tcccgccatg gcgttctcaa tctcagtgtc
    20521 gctgggcttc ggtgtctccg aaagcaacgc aaccgcctgc atgatctgtc ctgactggca
    20581 gtacccacat tgtgggacag agttttcgat ccacgccttt tgcacgggat gcagaccgcc
    20641 gggaccggct atggcttcga tcgtgagaat ttcctgtccc gaaacgccgc tcatgggagt
    20701 cgagcatgat cgcacggcct taccgttgcg atgcaccgtg caggctccgc acaaacccat
    20761 tccgcaccca aacttggtgc cgctgagtcc ggctatgtcg cggacatacc acagcagtgg
    20821 catgctcgga tcgccctcga atgatcgctc ctgcccattg agtttgaact taatcatcac
    20881 tgaacctccg ggtgacgata atcgctcgca attccgtacc aggctgaacg gccgcccaaa
    20941 tattgatcgc tattctaatt caagattcgg gtcagctctg agcttgcgaa acaggagttg
    21001 cgtgacgcgg acctcgaatg tttg
//
LOCUS       DASRKK010000746         7434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_958390, whole genome shotgun sequence.
ACCESSION   DASRKK010000746 DASRKK010000000
VERSION     DASRKK010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958390"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..551)
                     /locus_tag="VFC15_17960"
     CDS             complement(237..551)
                     /locus_tag="VFC15_17960"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12099.1"
                     /translation="MIDAYVLYLDAHPDFRTISFGRHISTTITAGEAARTGLPAMLTT
                     FMVERLGFRFTPELQLTLNLASEGGERLIAYAYEQPTREERDLIIAETKKMLAGYLFS
                     GK"
     gene            complement(548..907)
                     /locus_tag="VFC15_17965"
     CDS             complement(548..907)
                     /locus_tag="VFC15_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM12100.1"
                     /translation="MSNQLPVDSLVSASIAANRRQPAQDRSLRTVARILDAASSLLTQ
                     MPLEDVTTRRIASEPGVSVGAVYRFFPDRQSIIDAIAVRHVLEFRGQVDKEVMRGPCY
                     SNSRIWKISPPPSCSIA"
     gene            complement(1182..1448)
                     /locus_tag="VFC15_17970"
     CDS             complement(1182..1448)
                     /locus_tag="VFC15_17970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HZM12101.1"
                     /translation="MTPWQLVYTKQAQKDARRLASAGLRPKAEQLLTLLSNNPFQTPP
                     PFEKLLGDLRGAYSRRINIQHRLVYQVFEEEHIVKVIRMWTHYE"
     gene            complement(1445..1687)
                     /locus_tag="VFC15_17975"
     CDS             complement(1445..1687)
                     /locus_tag="VFC15_17975"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZM12102.1"
                     /translation="MNTMNASEAREKLYRLLDETAIAHEPVLITGPRSNGVLVGEEDW
                     NAIQETLHLLSVPGMRESIREGLATPVDGCEKEPGW"
     gene            1853..2644
                     /locus_tag="VFC15_17980"
     CDS             1853..2644
                     /locus_tag="VFC15_17980"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM12103.1"
                     /translation="MPWGLERWHGGRALHFITFSCCRRQPLLSSARCRDLFLTVLEQV
                     RRRYRWVVLGYVVMPEHVHLLVSEPQQRPLATAMQALKLGFARRVLAEQRRRRRGAQA
                     DLFEHCAQRIWQARYYDFNVCTAHKRVEKLRYMHRNPGSPTSAGFALAGVEAGEAWSG
                     GSAGTVALEPLPRLRLPGGRTGSGERLERARAKAQKGDNFSPVMILTRVSKSARPGAP
                     ANTGFVAREKPCAGNVGHPPTHESERRTSLMLISTSGLRPGTMIS"
     gene            2748..3239
                     /locus_tag="VFC15_17985"
     CDS             2748..3239
                     /locus_tag="VFC15_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12104.1"
                     /translation="MLEILKLVVSILFGGAAGAFLNEWFRRRKGKVQRISLIERVNRS
                     ANPELEGITLARMVGEEPLRHLEELANLREYQLTLRNTSSINLQNIEIQFEFPSTDVE
                     SWVSRPSMSKTALVPIETDPTAQEETTFRWRIPHLPAGDSIEFTFRAVNPPSGNYEVA
                     LYN"
     gene            3217..3702
                     /locus_tag="VFC15_17990"
     CDS             3217..3702
                     /locus_tag="VFC15_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12105.1"
                     /translation="MRSPYTTSDAVIIEKVIGEPPPKTTSVSTGAWITITAAMAVLIG
                     LVWLVASGRLVNSSGEKFSAVKEGGCDLRVVSLYDQYGTQFNSPWRIKDRIFNAGTQG
                     CIIQFGQISQKGPFTIGPGDVFEREWIIERVPVLVRSDLSVGLSDGTMKKIPIQVYAE
                     Q"
     gene            4150..6084
                     /locus_tag="VFC15_17995"
     CDS             4150..6084
                     /locus_tag="VFC15_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12106.1"
                     /translation="MEGSTEVRSARLKELAQGMKLGTLTDAESLLLEKVPTGSCAICG
                     PSGDDKDKRNDASGGGEWERSRQICSQFLVWLCTSEEARKQIGWRGIQIYGAEITSLF
                     DLSFASVPFPLVFRRCRFKQGINLRRADLAELDLSGSLVHKFHADRLTVKNCLYLRSA
                     TIVGEASLLGSQIGGDLDGEGSALNNPGGMALMADGINVKGSIYLRNGFAAHGTVQLQ
                     NAQIGAVLDCDGATFSDASTSLDADEINVKGSVFFRSLTAGGEVRLVRAQIGNNIECD
                     GATIDSKNGRALNANAVSVSGSIFLRSRKNENEITPFKATGEVNLNTAHVEGQIDCSG
                     GIVIKSGAEAFEARGVVVKGSVFLCDKFEAEGKVHLQGAQIGGAFDCKGGNFVKATFD
                     LTDASAGTLIDSGLNFRPSGPSDESLTVWPVHGALFLDGFSYNRISSEGRIDVHQRIG
                     WLGRQAGTPFRLQPYLQLAKILRDSGDAKGSLRVLETMEELRRRDEQNTPRTGSCKGL
                     RKLADKVWSLLLKWTIGYGYYPQRAIGALLILFALGWACYFVGYYWSKMVPAESSAYK
                     EYASKGTLPAYYPRFSAGIYSLENSLPLVKLGQADKWQPDPTSQSPRILIWFLRIQIL
                     LGWMLATFFVAGVTGVVHKQ"
     gene            complement(6387..6806)
                     /locus_tag="VFC15_18000"
     CDS             complement(6387..6806)
                     /locus_tag="VFC15_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12107.1"
                     /translation="MSEPQRNLTVDALEVIDGRDHEKIEGWIPELATEAELRDALEKA
                     FDYRGDVTITRKDGSRVEGYLFDRRNGPTLDASVVRLIPQNSAQKISIPYSDIAALAF
                     TGRDTAAGRSWEAWVRKYWERKAAGEKGIALEPEKLD"
     gene            complement(6940..>7434)
                     /locus_tag="VFC15_18005"
     CDS             complement(6940..>7434)
                     /locus_tag="VFC15_18005"
                     /inference="COORDINATES: protein motif:HMM:NF023372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HZM12108.1"
                     /translation="KAPDQKHFLGRARTRRLFRAILANRKAKWVFNQSPLFLEFLMGK
                     RDYPCTPWGMPTFNMFGWQKPCYLLQDGYADTFEELMRETEWHKYGTESGNPQCANCM
                     VHSGYEASAVHDTFGSLRGFLGTVRATFSSKYKDDEALAMLKETTLPVHAYDPLVQIG
                     NVES"
BASE COUNT         1576 a         1981 c         2265 g         1612 t
ORIGIN      
        1 tcccgaggca ggcgacatcg tctttcgcca taccctgctc gagttgtccc tgctgacggt
       61 gatggtcctg gtgctggtgg gatcgctaat cctgttgcaa aagtattccc acaaagccag
      121 tgaaagcgcg ttggacacct agccacgctc gaaacggacc cttccgaaat tgtgattctg
      181 gaacctcgcc caaggaccgg ggcgcaacct ggggcaccaa gcagttgcgg tgaaactcac
      241 ttgccggaga acaagtatcc cgccagcatc ttctttgtct cggcgatgat gagatcgcgc
      301 tcctcgcggg tgggctgctc gtaggcgtaa gcaatgaggc gctcgccgcc ttcgctggcc
      361 aagttcagcg tgagctggag ttcgggggta aagcggaagc ccaggcgctc gaccatgaag
      421 gtagtgagca tggcgggaag accggtgcgg gccgcctctc ctgccgtaat ggtggtgctg
      481 atgtggcgcc cgaaagaaat ggtgcggaaa tcggggtggg cgtcgagata gaggacgtag
      541 gcgtcgatca tgcgattgag cacgacggtg ggggcgaaat tttccagatt ctggagttgc
      601 tgtaacaggg gccgcgcatg acctccttat ccacctgacc gcgaaattcg aggacgtggc
      661 ggacggcaat ggcgtcgatg atggactgcc tgtcagggaa gaagcggtaa accgcgccga
      721 ccgagacccc gggttcggag gcaatgcgcc tggtggtaac gtcctcgagc ggcatttggg
      781 tgagcagcga gctggccgcg tcgagaatgc gtgctacggt gcgaaggctg cggtcctggg
      841 cgggctggcg gcgattcgcc gcgattgacg cactgaccag ggaatcaacg ggaagttggt
      901 tggacatgac cagagtcggg cgaatgcgaa tattgtatac ggcgcgcgaa acggggcagc
      961 aggcatccca gccaaaaccg ggaacaggtc gtaaattgca gcatgaacga aaattgggct
     1021 ggcgacgcta acagctttag gtgatgaggt gcgactcttc gggtttgtga aaccggcgca
     1081 gacgacgtgg catgaaaatc tatcgctctc tgtttcgaag tatagcgatc cctaggttag
     1141 cgccccaaag aaggacgcga acctggggca tcggggcacc tttattcgta gtgggtccac
     1201 atgcgaatta ccttgacgat atgctcttct tcgaaaacct gatagacaag cctgtgctgg
     1261 atgttgatgc ggcgggaata tgcgcctctc aggtcgccta gcagcttctc aaacggcgga
     1321 ggcgtttgga atgggttatt cgatagtagc gtgagcagtt gttcggcctt cggtcgcagg
     1381 ccggcggagg caagacggcg agcatccttc tgagcttgtt tggtgtagac aagctgccaa
     1441 ggcgtcacca gcctggttct ttctcgcacc catccaccgg cgtcgcgagt ccttcgcgga
     1501 tggattcacg catgccagga accgacagaa gatgaagggt ctcctggatg gcattccaat
     1561 cttcttcgcc aacgagtacg ccattggagc gtgggccggt gatgagcacc ggctcgtggg
     1621 cgatcgccgt ttcatcgagc agccggtaca gcttctcccg ggcctcgctg gcattcatcg
     1681 tattcatgtg tgtcagcgta ccctttttcg tacgttcagg caagcaacgg cgccttgagg
     1741 tcgggtgccc cgggtctcgc gcttttcgag acccgggaag tccgtcatcg atcacttgca
     1801 tagtgtgctc gcaatcacag ctcaaggcaa cggttagttc caccctgctg gcatgccttg
     1861 gggcctggaa cgctggcatg gcggacgcgc cctgcatttc attaccttca gttgctgtcg
     1921 ccggcagccc ctgctaagca gcgcgcgatg ccgcgattta tttctcacgg tgctggagca
     1981 ggtacggcgg cgctaccgct gggtggtgct gggatacgtg gtaatgccgg aacacgttca
     2041 tcttttggtc agcgagccac agcagcggcc gctggcgacc gccatgcagg ccctcaagct
     2101 cggcttcgcg cggcgggtgc tggccgagca acgcaggcgc cgccgcggtg cgcaggcgga
     2161 cctgttcgag cactgcgcac agcgcatctg gcaggcgcgg tactatgact tcaacgtctg
     2221 tacggcgcac aagcgggtgg agaagctgcg ctacatgcat cgtaaccctg ggtccccaac
     2281 cagcgccggt tttgcgctgg ctggggtgga agccggtgaa gcgtggtctg gtggaagcgc
     2341 cggaactgtg gcgttggagc cgcttccgcg cctacgccta ccaggaggca ggactggttc
     2401 gggtgaacga ctggagcgtg caagagctaa agctcagaag ggcgacaact tttctccagt
     2461 catgatactc acccgggtct cgaaaagcgc gagacccggg gcacccgcaa atactggatt
     2521 cgtggcaagg gaaaaaccat gtgcgggaaa cgtgggccac ccgcctactc atgaatccga
     2581 gcgtagaacc tccttgatgc tgatatcgac gtcaggttta cgtccgggca cgatgatctc
     2641 ctaagttgga ttgttaagag gatggctgtc gatatcgcca gatgattagg tgcttgtcaa
     2701 gacgggccaa aggcagagac aatgaagcgt cgaagaggtc gctttgagtg ctggaaattc
     2761 taaaactcgt agtgagcatc ctgttcggcg gcgctgccgg ggctttcctg aacgagtggt
     2821 ttcgtcgaag gaaggggaaa gtccagagaa tttcgctgat tgagagagta aacaggtcag
     2881 cgaatccgga attggaaggt attacgctcg caaggatggt tggtgaggag cctcttcggc
     2941 atctggaaga gctggctaat ctacgagaat accagttgac gctgcggaac acctcgtcga
     3001 tcaaccttca gaatattgaa atccagtttg aatttccatc tacggatgtg gaatcttggg
     3061 tttcgcgacc ctcgatgagc aagaccgctt tggttccgat tgaaactgat ccgaccgctc
     3121 aggaggaaac tactttccgt tggcgaattc cccacctacc agcaggcgat tcaatcgaat
     3181 ttacgtttcg ggctgtaaat ccgccgtccg gaaactatga ggtcgcccta tacaactagt
     3241 gatgcggtaa taatcgaaaa agtcattgga gaaccccctc ccaagacaac gtcggtgagc
     3301 acgggcgcgt ggataacaat tactgcagcg atggcggttt tgattggcct cgtttggctg
     3361 gttgcaagcg ggcgattagt caactcgtcc ggcgaaaagt tcagcgctgt aaaagaaggt
     3421 ggatgcgact tacgcgtggt ctcgctttat gaccaatatg gtactcagtt caacagtccg
     3481 tggaggatta aggaccgcat ttttaatgcc ggtacacagg gttgcataat ccagtttggg
     3541 cagattagtc agaaaggacc attcacaatt gggccgggtg acgtgtttga gagggagtgg
     3601 ataatagaaa gggtaccggt attggttaga tccgacctgt cagtcgggct gtcggacggg
     3661 acgatgaaaa agatacctat tcaggtctat gcggaacagt gatttggcgc cgggtgcccc
     3721 actcatccgc gggattcgta tgagtgggtg cctcgaatca cgagggtcca gtaccttcct
     3781 cgcagaccca aagtgacgac tccgctagct tcggttccat gacccaagcc tagagcgctg
     3841 gtacggcggc ttccacttgc caaggttgca ggttacgcgt agcgcaacta aattaggtgc
     3901 cgggtgcccc acacattcgc gggtttcgaa tgtgtgggat caagagtcgg tcaagctact
     3961 tggactccca ctcattccaa aagcaggaat gagtggggca cccggccacc tgcggtccaa
     4021 cctatcagcg aagctgagaa gcccatcatg tcgagagtac ggaaggacac ctaccgagct
     4081 ctcttgtagg gggtgcaact gagggcggac tcatggtata aacagcgtgc ttcccgacaa
     4141 ggaggtgcca tggagggttc tacggaagtg cggagcgcca gactgaagga attggcgcaa
     4201 gggatgaagc taggcacgct taccgacgcc gagagtctgc tgttggagaa ggtgcctact
     4261 ggcagctgcg caatttgcgg tcccagtggg gacgataagg acaaaaggaa cgatgcgagt
     4321 ggcgggggcg aatgggaaag atcccgccaa atctgctctc agtttctggt ctggttgtgc
     4381 acaagcgagg aggcccgcaa acaaattggc tggcgaggta ttcagatcta tggggccgag
     4441 atcacaagct tatttgattt atcgtttgct agtgtcccgt ttccattggt atttcggcgt
     4501 tgccgattca aacagggcat caacttgcgc agggccgatc tggcagaact ggatctttct
     4561 ggcagcttgg ttcataaatt ccatgccgat cgcctgacgg tgaagaactg tttatatctt
     4621 cgcagcgcta ccatcgtcgg cgaagcatcg ctgctcgggt cacaaattgg tggggatctc
     4681 gacggcgaag gcagtgcgtt aaacaacccg ggcggcatgg cgctaatggc agacgggatc
     4741 aacgtcaaag gtagtatcta cctccgaaac ggcttcgcag cccatggcac ggttcaactt
     4801 cagaatgccc aaatcggcgc tgttctcgac tgtgacggtg caacattcag cgatgcaagc
     4861 acgtcgctgg acgctgacga aatcaatgtg aagggttctg tcttctttag gagcttgacc
     4921 gccggcggtg aagtgcggct tgtgcgtgcc cagatcggca ataatatcga gtgtgatggc
     4981 gccacaatcg acagtaagaa tggtagagcg ctcaacgcca atgccgttag cgtaagcggc
     5041 tcaatatttc ttcggagccg caagaacgaa aatgagataa cgcctttcaa ggcgacgggc
     5101 gaggtaaatc ttaacactgc acatgtggaa gggcagatag actgctcagg cggtatcgtc
     5161 atcaagtccg gcgcagaggc attcgaagcc cgaggcgtcg tcgtgaaggg ttcggtgttc
     5221 ttgtgcgaca agttcgaagc ggagggtaag gtgcatctgc agggcgccca gattggtggc
     5281 gcctttgatt gcaagggcgg aaactttgtc aaggcaactt tcgacctgac ggatgcctcg
     5341 gccggcacgc tgatcgattc cggcctgaat tttcgtccat ccggcccttc cgacgagtct
     5401 ctaacagtct ggccagttca tggagcactc ttcctagacg gtttcagcta caaccgtatt
     5461 tcatccgaag gccggatcga cgttcaccag agaatcggct ggctggggcg acaagccggg
     5521 acgccgtttc gactacagcc ctacctgcaa ctggcaaaga ttctgcggga ttccggagat
     5581 gcgaagggct ccttgcgtgt actggaaaca atggaagaac tgcgccgacg tgacgaacaa
     5641 aatacgccca gaaccggttc ctgcaaaggc ctccgcaagc tggccgataa ggtctggagt
     5701 ctgctcttaa agtggacgat cggctacggc tattatccgc aaagagcgat cggggctctc
     5761 ttgatactgt tcgcacttgg ctgggcttgt tattttgtgg gctattactg gagcaaaatg
     5821 gttccggccg agagctctgc gtacaaagag tacgcaagta aaggaaccct tccggcatac
     5881 tatcccagat tctccgctgg gatctattcc ttggagaatt cgcttccgtt ggtgaagctc
     5941 gggcaggctg acaagtggca gccggatcct acatcgcagt ctccacggat cctcatttgg
     6001 tttctccgca tccagatcct tctcggctgg atgctggcta ccttctttgt tgcgggcgtt
     6061 actggtgtcg tacacaaaca gtgaggtaat acgaacgtcg tctccgctgc cccatccctc
     6121 agggcagggt cttgtccgcc gcctttggag gataggatgg gagtaaagat cgtgtgtcgc
     6181 cggctgaagc cggctcaaga ttcattatga ccagcttacc caggactccg ctacgctgcg
     6241 tcctgggcta accccatctc caccccttcg gggttcgagc attcgcaatt ccagagcgga
     6301 gcgaaccggg cccgtgcggc tttgcgcagc gcgcagcgaa tgcgggctga caactccagg
     6361 ccagagacga tcaatcacgt ctcggtctaa tcaagcttct ccggttccag cgcaatgccc
     6421 ttttcgccgg cggccttcct ctcccagtac ttgcgcaccc aggcttccca gcttctgccg
     6481 gcggcggtgt cgcgcccagt gaacgccaga gcggcgatgt cggaatacgg aatggaaatc
     6541 ttctgcgcgg aattctgcgg aataagccgt acgactgagg cgtcgagcgt cgggccattg
     6601 cggcggtcga agaggtagcc ttccacgcgc gatccgtcct tgcgggtgat ggtcacgtcg
     6661 ccgcgatagt cgaaggcttt ctccagcgca tcgcgcagct cggcttcggt ggccagctcg
     6721 ggaatccagc cttcgatctt ttcgtggtca cgtccgtcga tgacttctaa cgcatcaacg
     6781 gtcaagttcc gctgcggttc gctcatgatt tcgctgttcg cctttcgctc ttcgctactc
     6841 gtcaaagttc atgttcacca cggagaacac ggaggtcacg gagaaattca tttcattttt
     6901 cctccgtgct ctccgtgtcc tccgtggtga aaatactgct caggactcga cattcccgat
     6961 ttgcaccagc ggatcgtaag cgtgtaccgg aagcgtcgtc tccttcagca tggcgagcgc
     7021 ttcgtcgtct ttgtatttgc tggagaaggt ggcgcgcacg gttcccagaa agccgcgcag
     7081 cgagccgaag gtatcgtgca ccgcggaagc ttcgtatccg ctatgcacca tgcaattggc
     7141 gcactgcgga ttgcccgact cggttccgta cttgtgccac tcggtctcgc gcattaactc
     7201 ttcgaaggtg tcggcgtagc cgtcctgcag caggtaacac ggcttctgcc agccgaacat
     7261 gttgaaggtc ggcatgcccc acggcgtgca cgggtagtcg cgcttgccca tcaggaattc
     7321 caggaacagc ggcgactggt tgaacaccca cttggctttg cggttcgcca ggatcgcacg
     7381 gaacaaccgt cgcgtccgcg cccgtcccag aaaatgcttc tggtcggggg cctt
//
LOCUS       DASRKK010000747         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_273515, whole genome shotgun sequence.
ACCESSION   DASRKK010000747 DASRKK010000000
VERSION     DASRKK010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273515"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..2807
                     /locus_tag="VFC15_18010"
     CDS             324..2807
                     /locus_tag="VFC15_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM12109.1"
                     /translation="MAAALLVFSGAIFASGPNDAKTFVVAVTVIDEKNQSVPDATVEV
                     HVGDKSLSTSATDASGKVNLTLPGVGDYALAISKQGYLNTGTMLEIGADTPVQDMDVV
                     MSSAALSQQSVTVTGEASNPVTETESSQKTLPLEQAKISSTRPATLTDTLPLIPGIVR
                     AKDGSVRIAGFGEDHSALLVNSVDVTDPATGSFGLSVPIDSVQTIQVSEMPYLAQYGK
                     FTAGVVSAETRRGGDKWAYSLNDPFPDFRIRSAHMEGIADASPRFNLSGPLVKNKLYF
                     VEGAEYLFNNQEVRTLPYPENLTRSTAFNSFTQLDAILTPRQTLTASFHFAPHSLRYA
                     GLDYFNPQPVTPDANFHESTAMLTHHWAIGGGLLQSTFAGTSVSSNVSPQGNANMVLT
                     PGGNGGNYFSYNSRSATRLVWMEQWTPRALQFHGQHIFQIGSVIGHSENEGHFLGDPV
                     TLQNANGQVIQQIRYVGGKPYYLADTEPSIYAQDHWILNPHLALDAGIRLERQTVTST
                     TRAAPRAGFVWSPGESGKTIVRGGIGVFYDSVPLDVYAFNSYPNQIITTYDAQGLPVG
                     PPVRYLNITAEAANSTFPFIERRSKSGNFAPYSTAWNLEFERTMKRWLMVRVKYLQSH
                     EQGMITLQPGVVQNQPAFVLGSSGWAQTRQAEFTARIGPAAPRQFFFSYVRQYAHGDI
                     NDAGSYLGNFPSPVIQSNLIASLPSEIPNRFLFWGTYGNLPHKVTLSPHIEYRNGFPY
                     QPTNVYQQWVPLTGPQSRYPNYFSLDMRVSKDVQVNLQHAVRLSFTVRNLTNHFNPLE
                     VHSNLADPQYGRFFGNYDRKFLFDFDFLF"
     gene            2939..3082
                     /locus_tag="VFC15_18015"
     CDS             2939..3082
                     /locus_tag="VFC15_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12110.1"
                     /translation="MRAKVTAEQSVVRDVGGGFPDCADHRRDLMLLILLALPHISDLN
                     LSN"
BASE COUNT          679 a          952 c          868 g          692 t
ORIGIN      
        1 gcctgtgtgc cgtcactgcc caaccacggg caacacgcta gcgatcaggc cacacgccga
       61 acattgcaag acaatgactt acaagctctg aactctggca agcctaatgc actatcaccc
      121 gcgtgactcc cgacgaaaca gctcgacggc agaccgaacc tgatctcgca aagccgggct
      181 gttttctcgc ctccattgct ggttttgtac gcagttcgga gtattcatcc gcgtgcgatc
      241 aacagttgga gcgtggatat gggtttattt gcgagttata cgacgtgcgt cccgtctgta
      301 tattcgctag gcggtggctg ctattggctg cggcgctcct tgtattcagt ggggctattt
      361 tcgcctcggg cccgaatgat gcaaagacct ttgtggtggc tgtcacggtc atcgatgaga
      421 agaaccagtc cgtccctgac gctaccgtag aggtccacgt cggcgacaaa tccttaagca
      481 cctcggcaac tgacgcctca ggcaaggtca atctcaccct tccaggtgtc ggagattatg
      541 cgctggccat cagcaagcag ggctacctca ataccggaac aatgctcgag atcggcgcgg
      601 acaccccggt tcaggacatg gacgtcgtca tgagcagtgc agctctcagt cagcagagcg
      661 tcaccgtgac gggcgaggcg tccaaccccg tcactgagac cgagagcagc cagaagactc
      721 tgccgctgga acaggcaaag attagttcga cccgtcccgc aaccctgacc gatacgttgc
      781 ctcttatccc ggggatcgtg cgcgccaagg acggttcggt gcgcatcgct ggtttcggtg
      841 aagatcacag tgcgttgctg gtcaattcgg ttgatgtcac cgacccggcg accggcagtt
      901 tcggcctcag cgtgcccatc gacagcgtgc agactatcca agtctcagag atgccctacc
      961 tagcgcagta tggaaagttc acggcgggcg tggtttcggc tgagacgcgt cgtggcgggg
     1021 ataaatgggc ctacagtctg aacgatccgt ttcccgattt ccgcatccgc agtgcacaca
     1081 tggagggcat cgccgatgct tctccccgct tcaatctgag tgggccgctg gtcaagaaca
     1141 aactttattt tgtggagggc gcggaatacc tcttcaataa tcaggaggtg cggacgctac
     1201 cttacccgga aaatctgacc cggtcgaccg ccttcaattc cttcacgcag ttggacgcca
     1261 ttctgactcc gaggcagacc ctaaccgcga gcttccattt cgctccccac agtttgcggt
     1321 acgccggcct tgactacttc aatccccagc cggtgacccc ggatgcgaat ttccatgaat
     1381 ccacggcgat gctgacccat cactgggcga ttggcggcgg tctgctgcaa agcacgttcg
     1441 caggtacgtc ggtttccagc aacgtttcgc cgcagggcaa cgccaacatg gtgcttacgc
     1501 cgggaggcaa tgggggaaat tatttcagct acaactcccg cagcgccacc cgcctggtgt
     1561 ggatggagca gtggacgccg cgcgccttgc agtttcacgg tcaacacatt ttccagatcg
     1621 gttctgtcat cgggcacagc gaaaatgaag gccactttct gggcgatcca gtcacgttgc
     1681 agaatgccaa cggccaggtc atacaacaga tccggtacgt tggaggcaag ccgtattacc
     1741 ttgccgatac cgagccctcc atttacgccc aggaccactg gatactgaat ccgcacctcg
     1801 cactggatgc gggaattcgt ctggaacgcc agaccgttac ttccaccaca cgcgccgcgc
     1861 cccgcgccgg tttcgtgtgg tcgccggggg aatccggcaa aaccatagtc cggggtggca
     1921 tcggcgtctt ctatgactca gtgccgctgg atgtctatgc cttcaacagc tatccgaatc
     1981 aaatcatcac cacttacgac gcgcagggct tgccggtcgg accgccagtt cgttatctca
     2041 acatcaccgc cgaggcggca aactcgacct tcccgtttat cgagcggagg tcgaagagcg
     2101 ggaacttcgc gccttacagt acggcatgga acctcgaatt tgagcggacg atgaagcggt
     2161 ggttaatggt ccgcgtgaag tacctgcaga gccacgagca gggcatgatc acactgcagc
     2221 ccggggtcgt ccagaaccag cctgccttcg tgctgggatc ctcgggctgg gcgcaaaccc
     2281 gccaggccga gttcacggcc cgtattgggc ccgccgcgcc gcgccagttc ttcttctcct
     2341 acgtgcggca gtatgcccac ggtgatatca acgacgcggg ctcatacctg gggaactttc
     2401 cttcgccggt gattcaatcg aacctgattg ccagcttgcc cagcgagatt cccaaccgct
     2461 tcctgttctg gggaacctac ggtaatctgc cccacaaagt cacgctctct ccacacatcg
     2521 agtatcgcaa cggatttcct taccagccga cgaatgttta ccagcaatgg gtgcctttaa
     2581 ccgggccgca gtcccgctac ccgaattact tctcattgga catgcgcgtg tcgaaggacg
     2641 tgcaggtgaa tctgcagcac gcggtacgac tatcttttac ggttcgcaac ctgacgaacc
     2701 acttcaatcc cttggaagtg cattccaacc tcgccgatcc gcagtacgga aggttcttcg
     2761 gcaactatga tcgaaagttc ctatttgatt tcgacttctt gttctaaacc aagagcgatg
     2821 cgtgtaatta tgggaatcgg agataaggat ctacaaaagg ccgttgccaa actctttaag
     2881 agttatccgc ctcagcccag caagtagcgc ccaaggcaca aaccaacgag aagcgctggt
     2941 gagagcgaaa gtgactgctg agcagtccgt ggtgcgcgat gttggcggcg gttttcccga
     3001 ctgcgcggat catcgccgtg atttgatgtt gttaattctg ctggccttac cccacatttc
     3061 cgatttgaat ctgagcaact gagtttatga tgcccctgga agccttgatt catgcgggtt
     3121 tcaacatgac cgaggcgctc tggaggtgca cacccagtgg gtgagaatca aaatcagcct
     3181 tttcagctct c
//
LOCUS       DASRKK010000748         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_666278, whole genome shotgun sequence.
ACCESSION   DASRKK010000748 DASRKK010000000
VERSION     DASRKK010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666278"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFC15_18020"
     CDS             <1..381
                     /locus_tag="VFC15_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12111.1"
                     /translation="DGDQVVGSRTRVKIVKNKTAAPFREAEFDMMYGEGISREGDLLD
                     LAVTGSLVEKSGSWFSYKGERIGQGRENAKQFLKENKDIAAKIETDVRKSLGLISVED
                     KAQAAAANAEAAPNATPKGPAGRK"
     gene            711..1232
                     /gene="coaD"
                     /locus_tag="VFC15_18025"
     CDS             711..1232
                     /gene="coaD"
                     /locus_tag="VFC15_18025"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZM12112.1"
                     /translation="MKDIVAIYPGSFDPVTNGHLDLIHRGSKLFDRLIVAIARNLDKD
                     EALFEMEERKDMLEAMTSELDNVTVDIFEGLLVVYCIQKNANAVLRGIRAVSDYEFEL
                     QMALMNRKMEPRVETVFMMPAEKYSYLSSRIVREIAKFGGPLTGLVPELVEERVRHKV
                     AEKHSFATRTHLL"
     gene            1244..2443
                     /locus_tag="VFC15_18030"
     CDS             1244..2443
                     /locus_tag="VFC15_18030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM12113.1"
                     /translation="MTAAGTQLSSFLTNRIQRIEVSATLAVVSEAEKLRAQGADLVDF
                     GAGEPHFATPAHIKEAAIDAIHNNFTKYTAVGGTAELRDAIAQRHAADFGSDYKREDC
                     IASVGGKHALFNAIQVLVDHGDEVILPVPYWVSFKDIVRYAGGEPVYIATDEKHDFDL
                     TADMVERAITPRTKVIVLNSPGNPSGAVMKPDHMTSVLKLAHDRGIYVVSDECYVYLN
                     FTGQLFSAGCVHEVKERLIIIGSLSKTYAMTGWRLGYALGPAPIVSAMQKLQSQSTSN
                     PTSIVQKAAVAALTGSQECVQEMLAGYISLRDKVVDGLRAIPGLTCASPNGAFYAYPN
                     VSHYFERGGLNSASDVARKLLHEAHVVVVPGEGFGTQDHIRLSYAVSAENLERGLDRM
                     RKFFANV"
     gene            2448..3434
                     /locus_tag="VFC15_18035"
     CDS             2448..3434
                     /locus_tag="VFC15_18035"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZM12114.1"
                     /translation="MSELYPLLLMPIFDERPWGVRDLRPIYTRVVKEPIGESWLTWED
                     NRVANGPLAGRTLGELSKQYGRDLVGQAARFDDRFPLLVKFLFPGDKLSVQVHPDDEQ
                     AHKIGQPCGKTECWYALRAQPGAQVALGLKPGVTLERFKQAIQDTRAEELLNWVDVHQ
                     GDMLYVAAGTVHTIGGGMILVETQQTSDTTYRLYDYGRGRELHIAEGLAATKLQTTAG
                     KVVPRKSDSPNVLVRSPFFEVEKIKLREAMHTEVAAAFPHILVAVDGAGIVESQGMEP
                     ISFATGEAVVIPARVREYTVRPQWELDVMRMSLPTEAVAEPETVLGQSVGAP"
     gene            3483..>3792
                     /locus_tag="VFC15_18040"
     CDS             3483..>3792
                     /locus_tag="VFC15_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZM12115.1"
                     /translation="MATSGKFSFYPVILAGGRGTRFWPLSRKRMAKQLLPLNSKKSMI
                     QETVERLSPLAKPKQFWIITNEDLRPAILRQLKGLPKKHIVAEPAGRNTAPAIGLAAF
                     L"
BASE COUNT          794 a         1112 c         1157 g          729 t
ORIGIN      
        1 gatggtgacc aagtcgtggg ctcgcgcacc cgggtaaaga tcgtgaagaa caagaccgcg
       61 gcgccgttcc gcgaagccga gttcgacatg atgtatggcg aaggtatctc gcgcgaaggc
      121 gacttgctcg acctggcggt cactggaagc ctggtggaga agtcgggttc gtggttcagc
      181 tacaagggcg aacgcatcgg ccagggccgc gagaacgcca agcagttcct gaaggaaaac
      241 aaggacatcg ccgcgaagat cgagaccgac gtacgcaagt cgctcggcct gatctcggtg
      301 gaggataagg cgcaagccgc ggcggccaat gcagaagccg cacccaatgc tacgcccaag
      361 ggtccggcgg ggcggaagta aggcattgtg caacaggggc cagtttcaga gttggtgcag
      421 tccgggtaga gccagccttc aggctggcgt ccaagatcgc cccacacgat aaggcttggg
      481 ccgttgcggt cattttcaat gaccgcaacg gcccaagcgg ttcgtttatc cgcagcggac
      541 gcggcgctga agcgccgctc tacccgtact cgccggactg cgccaattct gaaactgccc
      601 taaggggttc tcttccggag gccctccgcg gcattctctt tccccgattg cctctgggcg
      661 caacccggaa taaaatcatc cggcataaac tcccgccttt ccgaggtctc ttgaaggata
      721 tcgtcgccat ctatcccgga tcgttcgacc cggttacgaa cggacacctg gacctgatcc
      781 atcgcggtag caagctcttc gaccggctca ttgtggcgat tgcccgcaac ctcgacaagg
      841 acgaagctct gttcgaaatg gaagagcgca aagacatgct ggaggcgatg acctccgaac
      901 tcgacaacgt gaccgtcgat atatttgagg gtctgctggt cgtctactgt atccagaaaa
      961 atgccaatgc ggtgctgcgg gggattcgcg cggtgagtga ctacgagttc gagttgcaga
     1021 tggcgctgat gaaccgcaag atggaaccgc gtgtcgaaac cgtgttcatg atgcccgccg
     1081 agaaatattc ctacctcagc tcacgcatcg tgcgggagat cgccaagttc ggcggcccct
     1141 tgactggact cgtgccggag ctggtcgagg aacgagtccg ccacaaggtt gcagaaaaac
     1201 attcattcgc gacgagaact catctcttat gaagggccga cccatgactg ctgccggaac
     1261 ccagctctcg tcgtttctga ccaaccggat tcaacgcatc gaagtgtccg ccaccttggc
     1321 cgtggtcagc gaggccgaga agttgcgtgc ccagggcgcc gacctggtgg acttcggcgc
     1381 cggagaaccg cacttcgcca cgcccgcaca catcaaagag gcggcgattg atgcgatcca
     1441 taacaacttc accaagtaca cggccgtcgg cggcacggcc gagctgcgcg acgcgatcgc
     1501 gcagcgccac gcggccgatt ttggctccga ctacaagcgc gaagattgca tcgcttcggt
     1561 cggcggcaag cacgcgcttt tcaatgccat ccaggtgctg gtggaccacg gcgatgaagt
     1621 cattctgccg gtgccgtact gggtttcgtt caaagacatc gtccgctatg cgggcggcga
     1681 gcccgtgtac attgccacgg atgagaagca cgacttcgac ctcacagcgg acatggtcga
     1741 gcgcgcgatc acgccgcgca ccaaggtcat cgtcctgaac tctcccggca acccgtcagg
     1801 cgcggtgatg aagcccgacc acatgacgtc ggtgctgaag ctggcccatg accgcggcat
     1861 ctatgttgtc tccgacgagt gctacgtgta cctgaacttc accggacaat tgttctcggc
     1921 gggctgcgtt cacgaagtga aggagcggct catcatcatc gggtctctgt cgaagaccta
     1981 tgccatgacc ggctggcgat tgggttatgc actggggcct gcgccgatcg tcagcgcgat
     2041 gcagaagctg caaagtcaaa gcacctcgaa ccctacgtcg atcgtgcaga aggctgcggt
     2101 tgcggccttg accggctcgc aggagtgcgt gcaggagatg ctcgccggct acatctctct
     2161 tcgcgataag gtggtcgatg gcctgcgcgc gattccgggc ctcacttgcg cctcgccgaa
     2221 cggggcgttc tacgcctatc ccaacgtcag ccattatttc gagcgcggcg gattgaactc
     2281 cgcttccgac gttgcgcgta aactcttgca cgaagcgcac gtggttgtgg ttccgggtga
     2341 agggttcggc acacaagacc acatccggct ttcgtacgcg gtttcagccg aaaacctgga
     2401 gcgcggactg gaccgcatgc gcaaattctt cgccaatgtg taggctgatg tctgaacttt
     2461 atccactcct gttgatgccc atcttcgacg agcggccttg gggtgttcgc gatcttcgtc
     2521 cgatttacac gagagttgtg aaggagccga tcggcgagag ctggctgact tgggaagaca
     2581 accgggtggc caatgggcct ctggctgggc gcacacttgg cgaactctcg aagcaatacg
     2641 gccgcgatct ggtcggacag gccgcgcgtt tcgacgatcg ctttccgctg ctggtgaagt
     2701 ttctctttcc cggcgacaag ctgtcggtgc aggtccatcc cgacgatgag caggcgcaca
     2761 agattggcca gccgtgcgga aagaccgaat gctggtatgc gctgcgcgcg cagcccggcg
     2821 ctcaagttgc actgggattg aagcccggag tgactttgga gcgattcaaa caggcgattc
     2881 aagatacgcg cgcggaagag ttgctgaact gggtggatgt tcaccaagga gacatgctgt
     2941 acgtcgctgc ggggaccgtt cataccatcg gcggcggaat gattctggtc gagacgcaac
     3001 agacatccga caccacttat cgcctgtacg attacggccg cggccgtgaa cttcatattg
     3061 ctgaaggatt ggccgcgacg aaactgcaga cgacggctgg caaagtggtt cctcgaaaaa
     3121 gcgacagccc taatgttctg gtgcgctcgc cgttttttga ggtcgagaag atcaagctgc
     3181 gcgaagcgat gcacacggag gtcgcagcgg ctttccctca catcctggtc gcggtggacg
     3241 gggcaggaat cgtagaatcg caagggatgg aacccatcag cttcgccact ggagaagcgg
     3301 tggtcattcc agccagggtg cgagagtaca cagtccgccc gcagtgggag cttgacgtca
     3361 tgcggatgtc gctgcccacg gaagcagtcg cggagccgga gaccgtgctc gggcaatcgg
     3421 tcggcgcgcc gtaatacact tacaagagcg ctcgccccga tgagcgcgag gaatcgaact
     3481 cggtggccac ttcaggtaag ttttcttttt atcccgtcat tctcgccggt ggacgcggca
     3541 cccgcttctg gcccttgagc cgcaagcgga tggccaagca actgctgccg ctgaacagca
     3601 aaaaaagcat gattcaggag actgtcgaac ggctatctcc gctggcgaag ccgaaacaat
     3661 tctggatcat caccaacgaa gacttgcgcc ccgcgatcct ccgccagctc aagggtcttc
     3721 caaaaaagca catcgtcgcc gaacctgccg gacggaacac ggctccagca attggcctgg
     3781 ctgcgtttct gt
//
LOCUS       DASRKK010000749         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_274598, whole genome shotgun sequence.
ACCESSION   DASRKK010000749 DASRKK010000000
VERSION     DASRKK010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274598"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFC15_18045"
     CDS             <1..216
                     /locus_tag="VFC15_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12116.1"
                     /translation="SLDQNVLSEVERTKGSSSASERVNQLLKYALETERKASLYQEAA
                     QFFDSAPEDRQERRAFQKAGLKSWSRL"
     gene            213..569
                     /locus_tag="VFC15_18050"
     CDS             213..569
                     /locus_tag="VFC15_18050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZM12117.1"
                     /translation="MTAWRRGPKPARWYPRRGEVCLIDLDKERPALVISSDSLNAHSL
                     DVCVLPISTAEHKAFSLRPKLKAGEGGLRRDSWVKCDQPTTVEKQLAIYPPLGTLASA
                     SMQRIETAVKAALDLR"
     gene            639..1019
                     /locus_tag="VFC15_18055"
     CDS             639..1019
                     /locus_tag="VFC15_18055"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZM12118.1"
                     /translation="MKSAAENKPTHIVKGNIFDALGFSPSEASALKIKAEILSAILEH
                     VRAKGYTQAELVDILDEYQPSVSNLLKGRISQVSIEKLLRYADRLHLQTSIAVRPMEA
                     KPHRRKSRAGPSARRATRKVAVAV"
     gene            complement(1145..1513)
                     /locus_tag="VFC15_18060"
     CDS             complement(1145..1513)
                     /locus_tag="VFC15_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12119.1"
                     /translation="MKVKINTDGFEGYAKRALGRARKLDRREAIKPQITITFDNPLAM
                     VEVLTAQRLRLVQQVRTKSSSITALAASLGRDPKSVRRDVLKLERAGVVRTREQINSG
                     HGRAKIVESVAREYRLTAVL"
     gene            complement(1510..1797)
                     /locus_tag="VFC15_18065"
     CDS             complement(1510..1797)
                     /locus_tag="VFC15_18065"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HZM12120.1"
                     /translation="MDETTQVRCALGKGTIREEVWQDFRGVVVKYNLAFINHSLCAED
                     QGRVLGYDNNHGHHHRHYAGAEEKFSFVSYEKLLERFLDEVRRLREKKEPI"
     gene            complement(2363..3169)
                     /locus_tag="VFC15_18070"
     CDS             complement(2363..3169)
                     /locus_tag="VFC15_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12121.1"
                     /translation="MPWSKSWLWIIPIAVLLISLAYIGSKQPDAGASSSGDLPEQQAN
                     KKWPELKFQSAGRLPTTIHGFRLGMSVAEALQVDSTLTNGINQNPSDEDYMLSRTLPS
                     GLDIELRFTGDRLYLINEKMWRISPDDATQVERNTYTMLGAANLSPFDWYGRAWVWID
                     GDVRARLSSEHTNPTGSPVHLSFELIAFPVVSKCAQMHPDSNMDMADVCARAKERWGE
                     APSEPIVARPMPTEMFGLQLGMVPWQVRDVIPGIDIYAVGVNSAGHDETG"
     gene            complement(3620..>4123)
                     /locus_tag="VFC15_18075"
     CDS             complement(3620..>4123)
                     /locus_tag="VFC15_18075"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZM12122.1"
                     /translation="GMAGILDSARFRAFIAMELKVSVENVTAFVLGGHGDTMVPMPRY
                     STVAGIPITELMDAATIARLVQRTRDGGAEIVKHLKTGSAYYAPSAAVTEMVEAILKD
                     KKKIVPCAAYLEGEYGISGLFVGVPCKLGSNGIEQIIQIKLTAEEQAALQKSADSVKE
                     LVGVIGV"
BASE COUNT          930 a         1178 c         1127 g          888 t
ORIGIN      
        1 tcgctcgacc agaatgttct gtcggaggtt gaacgcacca aggggagcag ttccgccagc
       61 gaacgggtca accagttgct gaagtacgct ctggagacgg agcgcaaggc cagcctctac
      121 caggaagcgg cgcagttctt cgacagcgcg ccggaagacc ggcaggagcg ccgcgccttc
      181 caaaaggccg ggcttaaatc ctggtcgcgc ctatgacagc ctggcgaaga ggaccaaaac
      241 ctgcgcgatg gtatccgagg cgaggcgaag tttgcctgat cgacctcgac aaggaacgcc
      301 ccgccctggt gatttcgagc gatagcctga acgcacattc tctggatgta tgtgtattgc
      361 cgatctccac cgccgagcac aaggcgtttt cactacgtcc taagctgaag gctggagagg
      421 gggggttgag gcgcgactcg tgggtgaagt gtgaccaacc gaccacggtc gagaagcaac
      481 tggcgatcta ccctccgctg ggcacgctgg catcggcatc catgcagagg attgaaactg
      541 cggtcaaggc cgcgcttgat cttaggtagt ccgtgacatc gaggtggcca agcagcgatt
      601 gaaacgggtg aagcagcgat tgcaggagca gaggacacat gaaaagcgca gcggaaaata
      661 agccgaccca tatcgtcaag gggaacattt tcgatgctct gggtttctct ccatcggagg
      721 cgagtgcctt gaaaatcaag gcggagattt tgtccgccat cctggagcac gtgcgggcca
      781 agggctacac gcaggcggaa ctggtggata ttctggacga gtaccagccg tcagtgagca
      841 atctgctgaa gggaagaatc tcccaggtga gcatcgagaa actattgcgt tatgcagacc
      901 ggctgcatct ccagacgagc attgccgtgc gccccatgga agcgaagccg caccgcagga
      961 aatcccgtgc gggaccgagt gctcgtcgtg ctacaaggaa agtcgctgtt gcagtctagc
     1021 tggcctcgaa tttcttctaa ggagcgtccg cgtcaggtgg gcagtagcct aggcaaaaat
     1081 tcccacccaa gcaaaggaag cttgggtggg gaacccggcg gatctgctga tgaagaagag
     1141 ttagctaaag aaccgcggta agtcggtatt cccgggccac tgattcgacg atcttagcgc
     1201 gtccgtggcc cgaattgatc tgttcccgtg ttcgtacaac tcccgcgcgc tccaacttca
     1261 gcacgtccct gcgtacagac ttaggatcac gtcccagcga cgccgcaagg gcggtgattg
     1321 acgatgattt tgtccgcact tgttggacaa gccgaagacg ctgcgcagtg agcacctcga
     1381 ccatcgcgag cgggttgtcg aacgtgatgg tgatctgcgg cttgatagct tcccgtcgat
     1441 cgagcttgcg tgcgcgtccc aaggcacgct tcgcgtaacc ttcaaatcca tccgtattga
     1501 ttttcacctt catatcggtt ccttcttctc gcgcagtctc cgcacttcgt ctagaaagcg
     1561 ctccagcagc ttctcgtagc tgacaaaaga gaacttctct tccgccccgg cataatgccg
     1621 atggtgatgg ccgtggttgt tgtcgtaccc caggaccctg ccctggtcct cggcacaaag
     1681 agagtgattg atgaacgcca ggttgtactt gacgaccaca cctcggaagt cctgccagac
     1741 ttcttcgcgt atggtcccct ttccgagagc acaccggacc tgggttgttt cgtccacaac
     1801 cttgcgtggt tttcccgctt tcttcaccca ttgctaggat atcacaggta gggcctaaga
     1861 agccctatct gccgggtcag actgatctgg aaaataacag gcaaaaaaac gcccacccca
     1921 gcaaaggaag cttgggtggg caccccggca ttgtgatcga gcacacggct cagaaaaacg
     1981 aatgcgtatc gggaaccgca ttgaaagttg tgggtgtatt atcaggcacc ttcactgaca
     2041 aacatcctgt tacatacggc ctattcgcgg aaccatcacc gttacggcgt tgaggttcga
     2101 tgtcaaaata tacctttctt gcatcatctt cccacgacac gacttcacct ttatccttac
     2161 ccttgacatc catggttggt gtgccaagat taagaataaa ggcactcgac ctgaagttct
     2221 cgacgtccga caacgagacg tcgtatgttg tatgacaaat cgtggaaaga tgcccttcta
     2281 agaacgacac gttgaattct gaatggcatg tcattgtccc ggaacaatca atacgctgcc
     2341 cggttgcatt atcgataaac cgtcaacctg tctcatcgtg cccagcactg ttcaccccta
     2401 cggcataaat gtcgattccg gggatcacgt cgcgtacctg ccaaggcacc atgccaagtt
     2461 gcaagccaaa catttcggtt ggcatcggtc tcgcgacaat cggctcggaa ggcgcctcac
     2521 cccatcgttc cttcgctctg gcacaaacat cggccatatc catattggag tccggatgca
     2581 tttgcgcaca tttagaaact acaggaaacg caataagctc aaaactcagg tggactggcg
     2641 atccagtggg gtttgtatgc tcgctggaga gccttgcacg cacatcccca tcaatccaga
     2701 cccacgctct accgtaccag tcgaatggac tcagattagc cgcacccagc atagtatagg
     2761 tgttgcgctc aacctgagtc gcatcatcag ggctaatccg ccacatcttt tcatttatta
     2821 aatacagtct gtcgcccgta aacctaagtt ctatgtcaag gccgctcggc aatgttcgcg
     2881 acaacatgta atcttcatcg gaaggatttt gattgatacc gttggtaagg gtgctatcaa
     2941 cttgaagagc ttcagccaca gacatgccca acctgaaacc atgaatcgtg gtcggcaatc
     3001 tgcctgccga ctgaaacttt aattcaggcc atttcttgtt agcctgctgc tcaggtaaat
     3061 ctccgctcga agaagcacca gcatcaggct gcttgcttcc gatgtatgcc agagaaataa
     3121 gcagtaccgc tatggggatg atccagagcc aagacttcga ccatggcatt gtcccagcag
     3181 gtaatagagg agtctgcgtg ctagggggtt ggctcagtcc aaactgccaa gcagccccag
     3241 atgacgcggc cgggtgaatt ggtggacttg aagaaacctt ttcggcatcc tcaaacgccg
     3301 aaggtggcgg agcgtcttct ctagccgcta ttagacttgc gcctgtggca gttccacaga
     3361 atttgcagaa cggactctga tccggcaaat cggccccaca cttggtgcag aacatgtggc
     3421 ctcccgcgac tctgtccgat gtgtttcccg aagtctttac ccgcgacacc cgttacaatt
     3481 tcacgcttgg tacagttggc ttgtcagtga cgaatgccgc tgtgggaagt gatggcggtg
     3541 ggtgcatcgg cagcgcacgc atgccccacc cttgtcgccc gatgttggcg acagggtggg
     3601 acgacatatc cgttgaaaac tacaccccga tcacgcccac caactccttc accgagtcgg
     3661 cgctcttttg cagggcggcc tgctcttccg cggtcagctt gatctggatg atctgctcga
     3721 tgccgttcga gcccagtttg cacggcacgc cgacgaacag cccgctgatg ccgtactcgc
     3781 cttccagata ggcggcgcac ggcacgatct ttttcttgtc cttgaggatg gcctcaacca
     3841 tctccgtgac cgcggccgac ggcgcgtagt aagcgctgcc ggtcttgaga tgcttcacga
     3901 tctcggcgcc gccgtcgcgc gtgcgctgca ccaggcgtgc gatggtggct gcgtccatca
     3961 actcggtgat gggaatgccc gccacggtcg agtaacgcgg catcggaacc atggtgtcgc
     4021 cgtgaccgcc gagcacgaag gcggtgacgt tctccaccga aaccttcagc tccatggcga
     4081 tgaaagcgcg gaagcgcgcc gagtccagaa tgccggccat gcc
//
LOCUS       DASRKK010000750        10031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_568951, whole genome shotgun sequence.
ACCESSION   DASRKK010000750 DASRKK010000000
VERSION     DASRKK010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568951"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1732)
                     /locus_tag="VFC15_18080"
     CDS             complement(305..1732)
                     /locus_tag="VFC15_18080"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZM12123.1"
                     /translation="MTQLESLSFDPRVTIRREGAPASNGRCVVYWMQRAQRALDNPAL
                     DLAVDVANALHQPVVIFFAPVPFYPHANLRHYAFLAQGIPDIAGRARQRGIGFVLRAD
                     PQHSLLKFCDEVKASIVIGDENPMREPRHWRALAAKRLGVPLWTVDADVIVPSKLLEK
                     EQYAARIIRPRLQQRLEQFQTAPRNPVAKVEWQAPRGLSALPDDASSDITEAWSGLDR
                     SVQPVDSFRGGTTEALKLLAEFVKHKLATYPENHGKPEIDGTSRLSPYLHFGHIGPHT
                     VLHAVLNSKASQPAKDDYINQFLTWRELSINFVHFNPLYDSIECAPDWAHKTLAAHAQ
                     DPRPILYTRDQFESAQTHDELWNAAQLQMLHAGWMHNYMRMYWAKKILEWSPSPAVAW
                     QTALYLNDKYFLDGRDPDGYAGVAWAIGGKFDRPWFERPIFGTIRWMSGDTARKKFDA
                     ARYIQQMRQLAEGVVNSYPPPPTCF"
     gene            complement(1905..2810)
                     /gene="folP"
                     /locus_tag="VFC15_18085"
     CDS             complement(1905..2810)
                     /gene="folP"
                     /locus_tag="VFC15_18085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZM12124.1"
                     /translation="MRAPFRWQLRSRALELGPRTLVMGVVNVTPDSFSDGGQFLDPQR
                     AVAHALQLLDEGADILDIGGESTRPGTRVLPDESGHKIAVSEDEELRRVMPVIEGILQ
                     ARPDAVVSIDTYKSGVARRAIEAGAEIVNDVSAFQWDASMPSTCSELNCGVVLMHTRG
                     QPAEWRKLKPESDITSLVVNDLASRVQVALDGSVARERIVLDPGFGFGKNFEENYPLL
                     AHFDHLQRLGFPLLAGTSRKSFIGRTVGKRRGREDAPPNERLYGSLAAMVTSILRGAH
                     IVRVHDVKPAVEAAAVADEILATEK"
     gene            2916..4502
                     /gene="dacB"
                     /locus_tag="VFC15_18090"
     CDS             2916..4502
                     /gene="dacB"
                     /locus_tag="VFC15_18090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZM12125.1"
                     /translation="MYRVRNLVHVVLLLTLAAHAFAADSAKSPHKKPDDGKKLGRAIE
                     QILSEPDVARGFWGIDVVSLNTGNRIFAFNENKLFTPASNTKLFTTAAVFGLIGPDYR
                     FKTTVESLGTVDKYGRLNSDLVVVGRGDPNLSGRTLPYYLHTERKALPIQVLIDLADQ
                     LVQKGLKYVDGDVVADDSYYVFERYGEGWSQDDLVWEWGAPVSALTINDNVLFVSIMP
                     ADRPGERAFLNVTPYPEYYRIDNRVMTTPQGTGPRKIYINREPGSNQITLWGNMPQDD
                     AGAGESLAIEDPADFTAKLFRELLEQRGVTVYGRPRTRHTELASMQTFSVTTIASGGG
                     GETGSHPNPVPLVLANHESQPLAEDLRVINKVSQNLHAELMLRLLGKEKGTSGSIAGG
                     LEVLRGFLTSAGIKPDQFVFYDGSGLSRENLVSPAAVVELLQYAQQQPWGKLYEDTLP
                     VAGVDGSLSDRLRTVPAKGAVHGKTGSLGHVNALSGYATTAKGERVAFSILCNNHNLS
                     NKKVIETIDQIVEKIVADGP"
     gene            4626..4913
                     /locus_tag="VFC15_18095"
     CDS             4626..4913
                     /locus_tag="VFC15_18095"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HZM12126.1"
                     /translation="MHVISRKKLREAAERHKDIASSLDAWYRLTNRAEWQSLADVRQL
                     FPSADAVGRFTVFNIKGDSYRLITEINYRGRRLYIRHVLTHAEYDKGAWKL"
     gene            4910..5308
                     /locus_tag="VFC15_18100"
     CDS             4910..5308
                     /locus_tag="VFC15_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM12127.1"
                     /translation="MSALHASADYARLLTKIPPKVIRSERENEYYARALYELDQRKSL
                     RREERELADLLTLLIEDFEERKYSLPKATPLETLAFLMEQHRLKQKDLVDVFGTRSIV
                     SEVINGKRELNKEHIRKLSQRFGVSPEVFF"
     gene            complement(5340..5768)
                     /locus_tag="VFC15_18105"
     CDS             complement(5340..5768)
                     /locus_tag="VFC15_18105"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZM12128.1"
                     /translation="MTVRLKRVYDPPSLHDGARILVDRLWPRGLTKEAAVLDAWLKDV
                     APSNELRKWFHAHPPQWSKFRQKYLAELTTGAAHAALQQLHDLARKRRGLTLLFASKH
                     VEQNNALVLKQLIDGERKPPTGTGPARAIAAGRARAARSR"
     gene            5850..6221
                     /locus_tag="VFC15_18110"
     CDS             5850..6221
                     /locus_tag="VFC15_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM12129.1"
                     /translation="MIRKQASRSILLVLILVAFTALMGCDSEPRKSDAELGLNPQQAQ
                     GRRVYDARCAECHYAYSKRDLRGPSLHGLFQKPFMSSGIPANDDRVTDIILLGRAKMP
                     GFQQKLTQQQLTDLLAYLHTL"
     gene            6279..7196
                     /gene="lipA"
                     /locus_tag="VFC15_18115"
     CDS             6279..7196
                     /gene="lipA"
                     /locus_tag="VFC15_18115"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZM12130.1"
                     /translation="MASVPELIQIDIAPRKGTPKPEWLKARAPMGENYHNLKTLARKL
                     DLHTVCESAHCPNIGECWNHHTATFMLLGNLCTRRCGFCAVPKGRPEAIDFEEPARVA
                     EAVEKLGLKFAVVTSVNRDDNNVGGATVFANTITEIRKRMPDCRVEVLIPDFQGLEEP
                     LQIVMDARPDILNHNTETVPRLYRVARSGARYERTLRLLERANEMNPAIPTKTGVMVG
                     LGESTDELLAVFRDLAARKVDILTVGQYLRPSKDHLPVARLYTPDEFRFLKDESMKMG
                     FRHVESGPLVRSSYHAHEQADAGTGVRVV"
     gene            7248..8087
                     /locus_tag="VFC15_18120"
     CDS             7248..8087
                     /locus_tag="VFC15_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12131.1"
                     /translation="MRSYILSLPAKMQPVVIVASTVAAAFVVSFFTRMFFSEQQLALD
                     NGLTTSVYGTLGTTYAVLIAFVVSGVWQSFSDAGQAVKSEANALTDLTFVIGSFSAEK
                     TRSIRESAKAYAQGVVERWDLLALAARDNVPTEEINLDTSSALVAAILSIRPEDAREV
                     ELYGQALDLMGIWLDARRSRLRSAEGNTAGALWGLLIAGAFVLFAFHGMFVTHAWAVW
                     AVLLLGFSFVLGLSFYLIFSLDSPFTGQLSAGPAPFLWLLKEFERSDGLGFTRADEAK
                     LRS"
     gene            8090..8521
                     /locus_tag="VFC15_18125"
     CDS             8090..8521
                     /locus_tag="VFC15_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZM12132.1"
                     /translation="MAGHLWDKSSHSSKTAPELGEWMNTRLLGGLALGIGAGVLAGMI
                     GIGGGLVIVPALVYFFKMEQHTAQGTSLAVLLAPSGLFAFMQYYKAGHVDVGLAIMIV
                     IGVFAGGYFGGSWAQQLSGPVLRKVFAVMMGIVAVNMFFQK"
     gene            complement(8523..9983)
                     /gene="hflX"
                     /locus_tag="VFC15_18130"
     CDS             complement(8523..9983)
                     /gene="hflX"
                     /locus_tag="VFC15_18130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZM12133.1"
                     /translation="MNSRSVDRFWHNSRSEPERVFLVGVEFLSRNLTSRDKRPRSSDA
                     RATDIDDVPAAHPRQQPPGKVPASARAARSVADPQLHAADADSRSFAAEESMAEFREL
                     ALSAGASIAGELHQRRQRPDSATLIGSGKVEELNGAVASVAPDLVIFDHELTPSQQRN
                     LERALHIRVIDRTQLILDIFARHARTREGQLQVELAQLEYMLPRLAGRGVEMSQLGGG
                     IGTRGPGETQLETDRRKIYRRVRHVKEQLENVRRIRGQQRQRRESVPVPVVALVGYTN
                     AGKSTLFNALTRAGVLESPRMFATLDPTLRHIKLPSKREILLSDTVGFIRHLPHTLVS
                     AFRATLEEVQRATLLLQVADVTSPAADEQHAQVEEVLRELEANDKPRLVVMNKLDLLP
                     ELKRESLIDSDDVVHVSAAKGIGMDRLLQRIDELIEQDPVRQVRLRVPQSEGKALSTL
                     DAKAIVLSREYREGYVELEVQAPESILRNLKTFIRN"
BASE COUNT         1988 a         2778 c         3271 g         1994 t
ORIGIN      
        1 cgcattacac tatgggtcgg cgtagccgga tttctcctct gggctgccgt tttcctgatt
       61 agcctgcgag tcacttggta gctaagtccc atttcgggaa ccagtggtta ctttcgtttt
      121 cttgatccca aaatttcagt gacttccgca agtcgtgtcg cgtatgaatc atgctggttg
      181 tttttgcccc gaagggcccg ctctcagctt cgacgaccgg ctaagttatt tgaaatctat
      241 caagtacgta taaacgcaat ggaatctcga acctgcactt gtttcgaaat gcaagtcgct
      301 tattctaaaa gcacgtaggg ggaggggggt acgaattcac aactccctca gcaagctgcc
      361 tcatttgctg gatgtaccgc gccgcgtcaa attttttgcg cgccgtgtcg cctgacatcc
      421 agcgaatggt gccaaagatg ggacgctcga accatgggcg gtcgaatttg ccgccaatcg
      481 cccatgccac tccggcgtaa ccatcgggat cgcggccatc cagaaaatat ttgtcgttga
      541 ggtagagcgc ggtctgccag gctacggcgg gcgaggggct ccactccaga attttctttg
      601 cccagtacat gcgcatgtag ttgtgcatcc atccggcatg gagcatctga agctgtgcgg
      661 cgttccagag ttcgtcgtga gtctgggcgg attcgaactg gtcgcgggtg tagagaatgg
      721 ggcggggatc ttgcgcgtga gcggctagcg tcttgtgcgc ccagtcgggc gcacactcga
      781 ttgagtcgta gagcgggttg aagtgaacga agttgatgga cagctcgcgc caggtcagaa
      841 actggttgat gtagtcgtcc ttggcgggct gcgacgcctt tgagttcaag acggcatgca
      901 ggacggtgtg cgggccaatg tgaccgaagt gcaggtaggg cgagaggcgg ctggtaccgt
      961 cgatctcggg cttgccatgg ttctcgggat aggtggcgag tttgtgcttc acgaattcag
     1021 cgagcagctt gagcgcttcc gtggtgccgc cgcgaaagga atccaccggc tgcacggagc
     1081 ggtcgaggcc gctccacgct tcggtgatgt cggaggacgc gtcgtcgggc agagccgaga
     1141 ggccgcgcgg agcttgccat tccactttcg cgacgggatt gcgcggtgcg gtctgaaatt
     1201 gttccaggcg ttgctgcagg cgcgggcgga taatgcgggc ggcgtactgc tctttctcta
     1261 gcagcttcga gggaacgatc acgtcggcat cgacagtcca caggggcacg ccgagacgct
     1321 tcgctgccag tgcgcgccag tggcgaggtt cgcgcatggg gttttcgtcg ccgatcacga
     1381 tcgaagcctt gacctcgtca caaaacttca gcagtgagtg ttggggatcc gcgcggagta
     1441 cgaatccgat gccgcgctgg cgggcgcgtc cggcaatgtc gggaattcct tgggcgagga
     1501 atgcgtagtg gcgcaagttg gcgtgcgggt agaacggcac cggcgcaaag aagatgacga
     1561 cgggctgatg tagcgcattc gcaacatcga ccgccaaatc gagcgcagga ttgtcgaggg
     1621 cgcgttgggc gcgttgcatc cagtaaacaa cacagcgacc gttggaggcg ggtgccccct
     1681 cacggcggat ggtgacacga ggatcaaagg aaagcgattc gagctgggtc atgggtgccg
     1741 tattgggatg ctagcagttc gctttcaccc tagcccgctg gcgcggtcca ggatggggca
     1801 cccacatcgt cgcggggaaa ggggtccttc gactccggcg gcaatgccgc cttcgctcag
     1861 gatgacacgt gagaacagat atgtgagtcg caacgctccg aaatctactt ttctgtcgcc
     1921 aagatttcgt cagccacggc ggcggcttcg accgcgggct tgacgtcgtg gacgcggacg
     1981 atgtgggctc cgcgcagaat gctggtgacc atggctgcga gagatccgta gagccgctcg
     2041 ttgggaggag cgtcttccct gccccgacgc ttgcccacgg tgcggccgat gaacgatttg
     2101 cgcgaggtgc cggcgagcaa tgggaatccc aatcgttgca ggtggtcgaa gtgcgccagc
     2161 agcggatagt tctcttcgaa attcttgccg aaaccgaatc cgggatcgag cacgatgcgc
     2221 tcgcgcgcga cactgccgtc cagggcgacc tgcactcggc tggcgagatc gtttactacc
     2281 agcgaggtaa tgtcggactc aggcttcaac ttgcgccact cggcgggctg gcctcgcgta
     2341 tgcatcaaca cgactccaca gttcaattca ctgcacgtac tcggcatcga agcgtcccac
     2401 tggaaggcgc tcacgtcgtt gacgatctca gcgccagctt cgatcgcgcg acgagccact
     2461 ccggacttgt aggtatcgat ggagacgacg gcatcgggcc gcgcctggag aatgccttcg
     2521 atgacgggca tgacgcgccg cagttcctcg tcttcggaga cggcgatctt gtggccggat
     2581 tcgtcaggca acacgcgggt gccggggcgg gtggactcgc ctccgatgtc gaggatgtca
     2641 gcgccttcgt caagcaactg gagggcgtgg gcaacggcgc gctgcgggtc gagaaactgt
     2701 ccgccatcgg agaacgagtc gggcgtgaca ttgactacgc ccatgaccag cgtgcgcggg
     2761 ccgagttcga gggcgcgaga acgaagctgc caacggaagg gagcacgcat cgttcgattg
     2821 taatttgtgc ggctctggac aggcgtgagg cagccaattc gcaaaggaac acaacgcaaa
     2881 ccgatgctag aattcaattg tggtgtagca gtcttatgta ccgggtccgc aatctcgttc
     2941 acgttgttct gttgctgacg ctggccgcac acgccttcgc cgccgacagc gccaaatccc
     3001 cgcacaagaa acctgacgac ggcaagaagc taggccgggc gattgaacag atcctcagcg
     3061 agccggacgt ggcgcgcggg ttctggggca tcgacgtggt ttcgctcaac accggtaacc
     3121 gcatattcgc cttcaacgag aacaagctgt tcacgcctgc ctcgaacacc aaactattta
     3181 ccaccgcggc ggtgtttggt ctgatcggcc ctgactatcg cttcaagacc acggtcgaga
     3241 gcctgggcac ggtggataaa tacggccgcc tgaactcaga cctggtggta gtggggcgcg
     3301 gcgatcccaa tctttcgggg cgcacgctgc cgtattacct gcacaccgag cgcaaggcgc
     3361 tgcccatcca ggtactgatc gatctggcgg accagttggt acagaagggg ctgaagtacg
     3421 tggacggcga cgtggtggcc gacgactcgt actacgtgtt cgagcgttac ggcgaaggct
     3481 ggtcgcagga cgacctggtg tgggagtggg gcgcaccggt gtcggcgctc acgatcaatg
     3541 acaacgtgct gttcgtgagc atcatgccgg cggaccgtcc aggggaacgc gcgtttctca
     3601 atgtcacgcc ctatcccgag tactaccgca tcgacaaccg ggtgatgacc acgccgcaag
     3661 gcaccgggcc gcgaaagatc tacatcaatc gcgagccggg atcgaaccag attaccttgt
     3721 ggggcaacat gccacaggac gacgcgggag cgggcgagtc actggcgatc gaggatccgg
     3781 cggacttcac ggcgaagctg ttccgcgaat tgctggagca gcgcggcgtg acggtgtatg
     3841 gacgcccacg cacccggcac actgagctgg cgtcgatgca gacgttcagc gtcaccacca
     3901 tcgcgtcggg cgggggcggc gaaacgggtt cgcatcccaa tcccgtgccg ctggtgctgg
     3961 ccaatcacga gtcgcagcca ctggcggagg acctgcgcgt aatcaacaag gttagccaga
     4021 accttcatgc ggagctgatg ctgcggttgc tgggcaagga gaaaggcacg tcaggctcga
     4081 tcgcgggcgg actggaagtg ttgcgcggat ttctgacttc ggcgggcatc aagcccgatc
     4141 agttcgtgtt ctatgacggc tcggggctgt cgcgcgaaaa tctggtgagt cccgcggcgg
     4201 tggttgagct gttgcaatat gcgcaacaac aaccgtgggg taagctctac gaagacacgc
     4261 ttccggtcgc tggagttgac ggctcgctga gcgaccgcct caggaccgtt ccagcgaagg
     4321 gcgccgtcca cggcaagacc ggttcgctgg gacatgtgaa tgcgctgtca ggctacgcga
     4381 ccaccgcgaa gggcgagcgc gtggcatttt ccatcctgtg caacaaccac aatttatcga
     4441 acaagaaggt catcgagacg atcgaccaga ttgtggagaa gattgtggcg gacggcccgt
     4501 aaactggaat ccgcctgcaa actcgcccca gaagttaagt gccacgcaat cttggctgtc
     4561 gatgcggatt cgcagattgc gaagtttctc cttgacaagt tcgcactttg cgaacatgat
     4621 gatgggtgca cgtcatcagc aggaagaaat tgcgcgaggc ggcggagcgc cataaggaca
     4681 tcgcaagttc cctggacgca tggtatcggc taaccaatcg ggcggaatgg caatcattgg
     4741 ccgacgtgcg gcagttgttt ccctcggctg atgccgtggg acgtttcacc gtatttaaca
     4801 tcaagggaga tagctaccgc ctgattacgg aaatcaatta tcggggcagg cggctttaca
     4861 tccggcatgt actgactcat gccgagtacg acaagggagc gtggaaacta tgagcgcatt
     4921 gcacgccagc gctgactatg cccggctgtt gacgaagata cctcccaagg tgattcgctc
     4981 ggagcgcgag aacgagtatt acgcccgggc gttgtatgaa ctcgaccagc gaaagtcact
     5041 ccgtcgcgaa gaacgcgagt tggccgacct gctcaccttg ctgatcgaag actttgaaga
     5101 gcgaaaatat tcgctgccca aggccactcc cctggagacg ctggctttcc tgatggagca
     5161 gcacaggctg aagcaaaagg acctggtcga tgtcttcggc acacggagca tcgtgtcgga
     5221 ggtaatcaat ggcaaacgag aactgaacaa agaacatatc cggaaactga gccagcgctt
     5281 tggagtctca ccagaagtat ttttctaacc atgcaagtaa gagattgttc cgcatccagc
     5341 tagcgggaac gggccgctcg ggcgcgtccg gcagcgatgg ctcgcgcggg accggtgccg
     5401 gtgggaggct tacgttcgcc gtcgatcaat tgcttcagca ctaaagcatt gttctgctcc
     5461 acatgtttcg atgcgaacag cagagtgagg ccgcggcgct tgcgggcgag atcgtgaagc
     5521 tgctgcaagg cggcgtgggc cgcgccggtg gtgagttcgg cgagatattt ctggcgaaat
     5581 ttgctccact gcggaggatg cgcgtggaac cacttgcgca actcgttgga aggcgcgacg
     5641 tctttgagcc aggcatcgag cacggcggct tctttggtca aaccgcgcgg ccagagccgg
     5701 tccaccagga tgcgcgctcc gtcgtgtagc gagggagggt cgtacacgcg tttgagccgg
     5761 accgtcatgc gaggtgtatt ctaacccgtt ggcagtgttg aaatcggtcc gcgtgcagga
     5821 atccggacga ttctacgggg aaaattggat tgatccgcaa gcaggcgagc cgttctattc
     5881 tgttagtcct catactagtt gctttcaccg cgttgatggg ctgcgattcc gagccgcgca
     5941 agagcgatgc ggaactgggg ttgaacccgc agcaggcgca gggccggcgg gtgtacgatg
     6001 cacgctgcgc cgagtgtcat tatgcctaca gcaagcgcga cctgcgcggg cccagcttgc
     6061 acggtctgtt tcagaaaccg ttcatgagca gcggtatccc ggccaatgac gaccgggtga
     6121 ctgacattat tctgctgggc cgggccaaga tgccgggatt ccaacagaag cttacgcagc
     6181 agcagttgac cgatctgctg gcgtatctgc atacactgta ggagcggcag ggctgcaacc
     6241 cgaaccgcag ccggagcgtc gtacaataga gcaatcgcat ggccagcgtt cccgaactga
     6301 tccagatcga cattgcgccg cgcaaaggca ctcccaagcc ggagtggctg aaggcccgcg
     6361 cgcctatggg cgagaactac cacaacctga aaacgctggc gcggaagctc gatctgcaca
     6421 cggtgtgcga gtcggcgcac tgtcccaata tcggggaatg ctggaaccat cacacggcga
     6481 cattcatgct gctgggcaac ctgtgcacgc ggcgctgtgg attttgcgcg gtgccaaaag
     6541 gacgtcccga ggcaatcgat tttgaggaac cggcgcgagt ggcggaagca gtggagaagc
     6601 tcggcctgaa gtttgccgtg gtgaccagcg tgaaccgcga cgacaacaat gttggcgggg
     6661 cgacggtctt cgcgaacacc atcaccgaaa ttcgcaagcg catgcccgat tgtcgggtgg
     6721 aagtgctgat ccccgatttc caaggcctcg aagagccgct gcagatcgtg atggatgcgc
     6781 ggccggacat cctgaaccac aataccgaga cagtgccgcg actctatcgc gtagcccgct
     6841 cgggcgcgcg ttatgagcgc acgttacgat tgctggaacg ggccaacgag atgaacccgg
     6901 cgatcccgac caagaccgga gtgatggtgg ggctgggtga aagcacggac gagctgctcg
     6961 cggtcttccg cgatctggca gcacgcaaag tggacatcct caccgtcgga cagtatctgc
     7021 ggccgtcgaa agaccacctg ccggtggcac gactgtacac acccgacgag ttccgcttcc
     7081 tgaaagatga atcgatgaag atgggattca ggcacgtgga gtcgggcccc ttggtgcggt
     7141 cgagctacca cgcgcacgag caggcggacg cgggcacagg cgtgcgggtc gtctagtttt
     7201 catctcagaa actttgctgc aagtcgttcg gttcccagtg aggaaacatg cggtcctaca
     7261 tcctgtcgct cccggcgaag atgcagccgg tggtcattgt cgcttcaacg gtggccgcgg
     7321 ctttcgtggt ttcgttcttc acccgcatgt tcttcagcga acagcaactg gcgctggata
     7381 atggattgac cacctcagtc tatggaacgc tgggcaccac ctatgctgtg ctgattgcct
     7441 tcgtggtttc tggcgtgtgg cagagcttca gcgacgccgg ccaggcggtc aagagcgagg
     7501 ccaatgcgct gaccgacctg acgtttgtca tcggcagctt ttcggcggaa aagacccgaa
     7561 gcatccgcga gtcggcaaag gcttacgcgc aaggggtggt cgagcgctgg gacttactgg
     7621 cactcgcggc ccgtgacaac gtgcccaccg aggaaattaa tctggacact tcctctgcgt
     7681 tggtggcggc gattctgtcg atcaggcccg aagatgcccg cgaagtggag ctgtacggac
     7741 aagcattaga cctgatggga atctggctcg acgcccgtcg cagccggttg cgcagcgctg
     7801 aaggcaacac cgcaggcgct ctgtggggct tgctaattgc aggagcattt gtgctgttcg
     7861 ccttccacgg aatgttcgtc acccacgcct gggcagtctg ggccgtgttg ctgctggggt
     7921 tttctttcgt gctagggctg tccttttacc tcatcttttc gctcgacagc ccgtttaccg
     7981 gccagctgag cgccgggccg gcgcccttcc tgtggctgct caaggaattt gaacggtccg
     8041 acggcctggg cttcactcgg gctgacgaag ccaagttgcg cagctagggg tggccggtca
     8101 tctgtgggac aagagctccc actcaagtaa aacggcaccc gaattggggg agtggatgaa
     8161 tacgcgtctg ctgggtggat tagcactggg gattggcgcc ggggttttgg ctgggatgat
     8221 tggtatcggc ggcggactgg tcattgtgcc cgcgctggtg tacttcttca aaatggaaca
     8281 gcacacggcg caagggacgt cgctcgcggt actgttggcg ccgtcagggt tgtttgcctt
     8341 catgcagtac tacaaggcgg gacacgtcga tgtggggttg gccatcatga tcgtgatcgg
     8401 ggtgttcgcg ggagggtact tcggaggaag ttgggcgcaa caactgtccg ggccggtgct
     8461 gcgaaaagtt tttgcggtga tgatggggat cgttgcggta aacatgtttt ttcagaagtg
     8521 agtcagttgc gaataaaagt cttcagattt cgcaggatcg attcaggcgc ctgcacttcg
     8581 agttcgacgt agccttcgcg atattcgcgt gaaagaacga tcgcttttgc atcgagcgtg
     8641 gatagcgcct tgccttcgga ttgcggtaca cgcagccgca cctgacgaac cgggtcctgc
     8701 tcgatcaact catcgatgcg ctgcagcagc ctgtccattc caataccttt ggcggcggag
     8761 acgtgaacga catcgtcgct gtcaatcagg ctctcgcgct tgagttcagg cagcaaatcc
     8821 agcttgttca tcaccaccag gcgcggcttg tcgttggcct ccagttcacg caggacctct
     8881 tccacctggg cgtgctgctc atcggcggcc ggactggtta cgtcggccac ctgcagcagc
     8941 aacgtggcgc gttgcacttc ttcgagggtg gcgcggaaag ctgacaccag ggtgtgcggc
     9001 aaatggcgaa tgaatcccac agtgtcagac aaaagaattt cgcgcttcga gggcagcttg
     9061 atgtgccgca atgtgggatc tagggtcgcg aacatgcgcg ggctttccag cacgccggca
     9121 cgggtaaggg cattgaacag cgtggatttg ccggcgttgg tgtagcccac gagggcgacg
     9181 accggcacgg gcacggattc gcggcgctgg cgctgctgtc cacgaatgcg gcgcacgttt
     9241 tcgagctgtt ccttgacgtg acggacgcgt cgatagatct tgcggcgatc ggtttcgagc
     9301 tgggtttcgc cgggtccgcg agtaccgatg ccgccgccga gctgcgacat ttcgacgccc
     9361 cgcccggcga gccgggggag catgtactcg agttgtgcca actccacctg caactgacct
     9421 tcacgggtgc gggcgtgacg agcaaagatg tcgaggatga gctgggtgcg gtcgatgacg
     9481 cgaatgtgga gtgcgcgctc gagattgcgc tgctgcgagg gcgtcagctc gtgatcgaag
     9541 ataaccaagt cgggcgcgac ggaggcaacc gcaccgttca actcttcgac cttaccactg
     9601 ccgatgaggg tagcggaatc gggacgctgg cgccgctggt ggagttctcc ggcgatgctg
     9661 gctccggcgc tgagagcaag ctcgcggaac tcggccatgg attcctcggc ggcaaaggag
     9721 cgagagtcgg cgtcggcggc gtgaagctgc ggatcggcaa cactgcgggc cgcgcgcgcg
     9781 ctggccggca ccttgcccgg cggctgttgg cgaggatgcg ctgctggcac gtcgtcgatg
     9841 tcggtggcgc gggcgtcgga ggaacgcggc cgcttgtcac ggctggtcag attgcgcgaa
     9901 aggaactcca ctcccacaag gaagacacgt tcgggttcgc tccggctatt gtgccagaag
     9961 cgatcgacgg atctggagtt cattccacca ggatgagaaa tatgctaact ctccggggtg
    10021 ggcgcagggg c
//
LOCUS       DASRKK010000751         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_667437, whole genome shotgun sequence.
ACCESSION   DASRKK010000751 DASRKK010000000
VERSION     DASRKK010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667437"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFC15_18135"
     CDS             <1..453
                     /locus_tag="VFC15_18135"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZM12134.1"
                     /translation="TLIAPKSGKLQAMKHDMKADYFDVDLVLERANPADFDAVMLPGG
                     ALNADALRVDQKAQEFIRRIDEAGKSIAVICHGSWLLVSAGLTKGRTLTSYHTIQDDI
                     RNSGGQWVDREVVRDRNWISSRQPSDLPAFNREMIALFSAKKASAHNS"
     gene            complement(919..1248)
                     /locus_tag="VFC15_18140"
     CDS             complement(919..1248)
                     /locus_tag="VFC15_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12135.1"
                     /translation="MIMAVCLVIYASTATVMFGTLLNRVPDPLTLMGVFHFMYTVMIA
                     LSAVCGILGIMAGLALLAERRSGRTLALVAGFLSLSNIPLGTTLGIYTLVMLLPIAAT
                     QRYGRSD"
     gene            complement(1518..>2520)
                     /locus_tag="VFC15_18145"
     CDS             complement(1518..>2520)
                     /locus_tag="VFC15_18145"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM12136.1"
                     /translation="RLKHEQMKESPFLFLRGTFYRWAQLWPALCAELCGAPKVLSVGD
                     LHVNSFGTWRDAEGRLCWGVDDFDESYPLAYTNDLVRLAASMKIVIDAGGLSVTPKEG
                     CDAILEGYRKSLKEGGRPVVLAEHEHKLGKLGVDSFKPPTDYWGKLNRLPVVTRPLRS
                     DVKRALEKTLPDPKMEYKLVRRQAGLGSLGQQRFVAIANWQGGSIAREAKAVLPSACT
                     WLEGKLGNGQSYYQQAISSAIRSHDPYQEIVGSWLIRRLSPDSNPIDIQDLPKRPDEQ
                     MLLHTMGSESANVHLGTKRQVHNILKDLQSRKPNWLRDAAKRMARAVEKEWKEYRKG"
BASE COUNT          589 a          696 c          667 g          568 t
ORIGIN      
        1 actttgatcg ctccgaaatc aggaaaactt caggcgatga agcacgatat gaaagcggat
       61 tacttcgacg tcgacctagt gcttgaaaga gcaaaccctg ctgattttga cgcagtcatg
      121 cttcccggtg gcgcattgaa tgctgacgct ctgcgggtgg accaaaaggc gcaagagttc
      181 attcgccgaa tcgacgaagc agggaaatcc atcgcggtca tctgtcacgg gtcgtggctt
      241 ctggtctcgg cgggactgac gaaggggcgg acattaacca gctatcacac catccaagat
      301 gacatccgca attccggcgg ccaatgggta gaccgtgaag tggtccggga ccgcaactgg
      361 atcagcagtc ggcagccttc tgacctgccc gccttcaacc gggagatgat tgcgcttttc
      421 agcgcgaaaa aagcaagtgc gcataactcc tgagcccagg tagcgcctgc ttgggatgtc
      481 gagttgttgg aaactcagca tgcgtggctg cccacagtaa tatcaaaggg gtgagagtaa
      541 tttgacaatg ttaagggaat attaacgcta gtcggtgagc aggcattgaa gtacaaattc
      601 gtcgattaga attgttccct tttctccggg ggtgctcccc aatgtgggtt ccagaaatat
      661 ttcgccgcac ccggagcact tccacgcaac gacgatgcct ccgagttggc ttaccgcaac
      721 gagatgatcc acgcccacag tctacgctgt tcgccttggc gctcaagcgc gtggatattg
      781 aaacagcgac ggggtgattt actcttgacg cgcctgcttc accatctcga caaacacttc
      841 tctggcctgc attgggtttg taacttctcg tcagaaagct atgcgggctc catgcattcc
      901 atcctgcgtt ttcaggcgtc aatcggaacg accgtaacgc tgcgtagctg ctattggaag
      961 caacattacg agtgtgtaaa tccccagagt tgtacccagc ggaatattgg agagcgacaa
     1021 aaaccctgca acgagggcga gtgtgcggcc tgatcgccgt tctgcaagta aagccagtcc
     1081 agccatgatc cctaaaattc cacagacagc cgacaaagct atcattactg tgtacataaa
     1141 gtggaaaaca cccatcagtg ttaacgggtc aggtacgcga ttgagcagag tgccaaacat
     1201 tacggtggct gtactggcgt agatgaccag acacaccgcc atgatcaatc gaatgactcc
     1261 gtaaactatc caacatattc ccaaagttcg gaggtgaccg ctcaggctcg gcgcaacggg
     1321 atcgtgtgag gacatgacgg cctcctgtgg gctggcgtgg ctggcaaagt ctaccactga
     1381 atggattgac cgcgggcaca gtgagaaatg acgtgctgaa ttacaagcgc aaatcgaaag
     1441 gatcctgcgt gagcgtccta tgagcgagtt gcctgagcca ggcgccttcg cgacccgctc
     1501 tcaggaccgg aaggccgtca acctttgcgg tattctttcc actctttttc cactgcgcgc
     1561 gccattcgtt ttgctgcatc tcgcagccaa ttcggttttc tgctttggag gtctttcaaa
     1621 atgttgtgaa cctgacgttt ggtgcctaga tggacattgg cagattcaga tcccatcgtg
     1681 tgtagaagca tttgctcgtc tggccgcttc ggcaaatctt gaatatcgat gggattggaa
     1741 tccggcgaaa gtcggcggat caaccaggat cctacgatct cctgataggg atcatgggat
     1801 cgtatcgccg aagagatcgc ttgttggtaa tacgactgcc catttcccag cttgccctcc
     1861 agccatgtgc aagcggaagg caggactgcc ttggcttctc gagcaatgct gcctccttgc
     1921 caattggcaa tcgccacaaa tctttgctgg ccaagactgc ctaacccggc ttgccggcgc
     1981 accaacttgt attccatctt cgggtcgggc aaagtcttct ccaaagcccg cttcacatca
     2041 ctcctcagtg gcctcgtgac aacaggcaag cggttcagct ttccccaata atccgtgggc
     2101 ggcttaaaac tatcaacccc caacttgccg agcttgtgtt cgtgttctgc cagaacgacg
     2161 gggcggcctc cctccttgag ggacttgcga taaccttcca ggattgcgtc acatccctcc
     2221 ttgggcgtca cgctgagacc gccggcgtct ataacgatct tcatgctggc ggccaagcgc
     2281 acgagatcgt tcgtgtaggc cagtgggtag gactcgtcga aatcatcgac accccagcag
     2341 aggcggcctt ccgcatctcg ccaagttcca aaactattca catgcaggtc gccgacggac
     2401 aaaaccttcg gtgcgccaca cagttcggca caaagtgccg gccaaagctg agcccagcgg
     2461 taaaaggttc cccgcagaaa caagaacgga ctttccttca tttgttcatg cttgaggcgg
//
LOCUS       DASRKK010000752         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_471546, whole genome shotgun sequence.
ACCESSION   DASRKK010000752 DASRKK010000000
VERSION     DASRKK010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471546"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFC15_18150"
     CDS             complement(<1..1140)
                     /locus_tag="VFC15_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZM12137.1"
                     /translation="MERGFHRGFTAAERTELWDRWKRGESLKAIGRAFGKPSSSIYFL
                     VAPHGGIRPAERRRSRLALTLAEREEISRGVTARRSARSIAKQLGRSPSTVSREMSSN
                     GGCDRYRATVADENAWARSRRPKCCKLAINSRLRQSVAGKLRLDWSPEQIAGWLKRTH
                     PEDECNRVSHETVYRSLFVQARGVLKKELLSHLRQKRSMRRSRPVDPNGDRRGHMKDA
                     VSIRQRPAAVEDRAVPGHWEGDLLSGPNDTYIATLVERQTRYVMLAKVSGKDTRTVVN
                     ALIKQAKKLPKELYKSLTWDRGKELTAHRRFTLATNIDVYFCDPQSPWQRGSNENTNG
                     LLRQYFPKGTDLSVHSQAYLNKVARQLNERPRETLQFETPAERFNA"
     gene            1329..2360
                     /locus_tag="VFC15_18155"
     CDS             1329..2360
                     /locus_tag="VFC15_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM12138.1"
                     /translation="MQTVTTIGLDIAKSVFQVHGVDAGGQVIVRRQLKRRYVLTFFQK
                     LSPCLVGIEACASAHHWSRELQGLGHTVRLMPPAYVKPYVKRQKNDATDAEAICEAVT
                     RANMRFVPTKTPEQQSGLVLHRTRHLFIRQQTSVINAIRAHLAEFGIVASVGRHGVEE
                     LLTVVADPNDRRVPEIARACLSALGAQLRRMKEQILEFDRLIRAWHRSNEMSMRLEEA
                     PGVGPVLATALVATVADPKSFRSGRNFSAWIGMVPKQHSSGGKNRLGNISKQGDRYLR
                     GLFVAGALSVIRYAKIHGTKHRPWLTALLARRPTKVAAIALANKIARMVWAMMARGER
                     YKEPVALAR"
BASE COUNT          495 a          761 c          728 g          522 t
ORIGIN      
        1 ggcgttaaat ctctctgctg gggtttcaaa ttgcaaggtc tcacgtggtc gttcgtttag
       61 ctgacgagcc actttgttca gataggcttg cgagtgcacc gataagtcgg tgcccttcgg
      121 aaagtactgt ctcagcaggc cattggtgtt ctcgttcgat ccgcgctgcc acgggctttg
      181 cggatcgcaa aaatacacat cgatgttggt tgccaacgta aatcgacgat gagctgtcag
      241 ttccttgccc cggtcccagg tcagggactt atacagctct tttggtagct tctttgcctg
      301 cttgatgagc gcgttgacta ccgtccgggt gtccttacct gacaccttgg ccaacatcac
      361 gtaacgggtt tgacgctcga ccaaggtcgc gatgtaggtg tcgttcggcc cggacagcag
      421 atcgccctcc caatggccag gcaccgcccg atcttcaacc gccgccggtc gctgacggat
      481 tgagacggca tccttcatat gtccccgtct gtcgccattc gggtcaaccg gcctggagcg
      541 acgcatcgag cgcttctgtc ggagatggct gagcagctct ttcttgagca cgccgcgcgc
      601 ttggacaaac aggctccgat atacggtctc gtgtgacacc cgattacact cgtcttcagg
      661 atgcgttctc ttcagccagc cggctatctg ctcgggtgac caatccaatc tgagcttccc
      721 tgccactgac tgccgaagcc gcgaattgat cgccaattta caacattttg gacgacgcga
      781 tcgcgcccag gcattctcgt ccgcaactgt cgcccggtag cgatcacagc cgccattgct
      841 gctcatttca cggctcaccg tcgagggcga gcggccaagc tgtttggcga tagaccgggc
      901 cgatcgacgt gccgtaacgc ctctggaaat ctcctcgcgt tccgcaagcg tcaatgccag
      961 cctggagcga cgccgctccg caggacgaat tccaccatgc ggagccacca gaaaatagat
     1021 cgacgaagac ggcttgccaa aagctcgtcc gatcgccttc agcgactccc cgcgcttcca
     1081 gcgatcccat aactccgtcc tctccgccgc agtaaatcct ctatgaaatc cccgttccat
     1141 cccacactcc atcttttccc tctaagataa agtgttgcgt cgaccggttg agaccaccgg
     1201 ggtaaaccgg aagtcggcgt cagccagccc gacttccgag tttgatgctg tggacggctc
     1261 ctccaccggc acatcggtgc catggatgtg ggcgctgttg aggcttccac gactcagagg
     1321 agcgagccat gcagacggta acgacgatcg gtttagatat cgccaagtcg gttttccagg
     1381 ttcacggcgt tgacgctggc ggccaggtga ttgttcgccg gcaattgaag cgtcgctacg
     1441 tcctgacgtt cttccagaag ctgtcgccgt gtctggtcgg tatcgaagcc tgcgcctcgg
     1501 cgcaccactg gtcgcgtgag ctacagggac tgggtcacac cgtccggctg atgccgccgg
     1561 cctacgtgaa gccctatgtc aagcggcaga agaatgacgc gacagacgcg gaggccattt
     1621 gcgaggcggt caccagggcc aacatgcggt tcgtgccgac caagactccc gagcagcaaa
     1681 gcggcctggt gctgcaccgc acgcgccatc tgttcatccg tcagcagacc tcggtaatca
     1741 atgcgatccg ggctcatctt gccgagttcg ggattgttgc gtcggtcggg cgtcacggcg
     1801 tcgaggaact gctcactgtc gtcgccgatc cgaacgacag gcgggttcca gagatagctc
     1861 gtgcgtgtct ctcggcactc ggggctcaac tgcgaaggat gaaggagcag atcctggagt
     1921 tcgaccggct gatcagggca tggcatcgat ccaacgagat gagcatgagg ctcgaagaag
     1981 cccccggcgt cggtccagtg ctggccactg ctttggtcgc taccgttgct gatccaaagt
     2041 ccttccgatc agggcgcaac ttctcggcct ggatcggtat ggtaccgaaa cagcactcca
     2101 gtggaggcaa gaacaggctc ggcaatataa gcaagcaagg tgaccgctat ctgcgcggcc
     2161 tgtttgtggc cggcgcgctt tccgtcatcc gttacgccaa gatccatggc accaaacatc
     2221 ggccctggct cacggccttg ctggcgcggc gtccgacgaa ggttgccgca atagcgctcg
     2281 ccaacaagat cgcgcgaatg gtctgggcca tgatggccag gggcgagcga tacaaggaac
     2341 ccgtcgcgct tgcgcgataa acgaaatcgc gccgggcaac cggcgtgatg tgaaggttgg
     2401 gagggcgaac agcacgtaat gcagagccgg tcgatccggc gatcaggaca acccacttgg
     2461 gtcaaagcat tgtcaaatgc gagagtttga ttgggacctg atccgc
//
LOCUS       DASRKK010000753         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_766159, whole genome shotgun sequence.
ACCESSION   DASRKK010000753 DASRKK010000000
VERSION     DASRKK010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766159"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFC15_18160"
     CDS             complement(<1..798)
                     /locus_tag="VFC15_18160"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HZM12139.1"
                     /translation="MSRKIFLLSFCFVAATTFAQQPSGQTAMPGTSPSNAPGAAQGQG
                     GPTLPYIPSLDVSAMDRSVDPCVDFYRYSCAGWEQKNPIPADQIRWDVYSKLYEDNVN
                     YLRGILEQASAAKSPDAVTQKIGDYYAACMDESAVEKLGAKPMQPALDQVQAAKNVHD
                     LAPVVARLHLDGDSLLFDSGSQQDPDNSDAMIVGVGQGGLGLPDRDYYTKDDAKSKEI
                     RARYLQHVQKMFELLGDPPATAKKEADTVMRIETSLAKASLTRVDRRD"
     gene            complement(1143..2417)
                     /locus_tag="VFC15_18165"
     CDS             complement(1143..2417)
                     /locus_tag="VFC15_18165"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12140.1"
                     /translation="MASSVSLSEYLRLTRENHNFRKLWFAQIVSELGDWFYTLAIYSL
                     LLDLTGRASSVALALVLQVLPQTFVGPLAGAINDRLSRRRVMIFADLMRMLIVGSMLL
                     VRSARFVWVIYPLLLLETVMAAFFEPARNSIIPSITVEEDVIVANTLASATWSFNLAI
                     GALMGGAVAALFGRDAVFVLNALSFLVSAVLIGRMHFHEAHLAAAGPVKIRELFDYSP
                     IVAGLRYISHNRKLLAMVSLKGGVGILGTAWVLFPVLGQRVLSLPFAISRERAALFGM
                     SLLMGARGLGALVGPLLASHWAGRRQQRLRSGIFLGFLLGGVGYVSLGLTHNLWVACA
                     VVMLAHTGASTVWVFSTTLLHLNTEDRFRGRVFSADLGIAMLMVAIASWTAGQAIDRG
                     IAPQVVASATGIAMLLPIVAWSFAMRMWRGES"
BASE COUNT          584 a          754 c          772 g          445 t
ORIGIN      
        1 atcgcgccgg tcgacccgcg tcaacgaagc tttcgccagg ctggtctcga tgcgcatgac
       61 cgtgtcggct tcttttttcg ccgtggcagg cgggtcgccg agcaactcga acatcttctg
      121 tacatgctgg agatagcgcg cacgaatttc tttggatttg gcgtcatcct tggtgtagta
      181 atcgcgatcc ggaagaccca gcccgccttg acccacaccg acaatcatgg cgtcggagtt
      241 atccgggtcc tgctgcgacc cgctgtcgaa gagcagggaa tccccgtcga gatggagacg
      301 cgccaccacc ggcgcgagat cgtgaacatt cttggccgct tgcacttggt cgagtgcagg
      361 ctgcattggt ttggcgccaa gcttctcgac ggcggattca tccatgcagg cggcgtagta
      421 atcgccgatc ttctgggtaa cggcatccgg ggacttggcc gcggatgctt gttcgagaat
      481 gccccgcaag taattgacat tatcttcgta cagcttcgaa tagacgtccc aacggatctg
      541 gtcggcagga atggggttct tctgctccca gccggcgcag gagtaacggt agaaatccac
      601 gcacgggtca accgagcggt ccatcgcgga aacgtcaagg ctggggatgt agggcaacgt
      661 gggcccacct tgaccttggg ctgcaccggg cgcattgctg ggactggttc ccggcattgc
      721 ggtctgcccc gagggttgct gagcgaaagt tgttgcggcg acgaaacaga aagaaagcag
      781 gaaaatcttg cggctcacgg ctgtcctccg agggaagggc aaggatagca gagccatacc
      841 accgacacga aaaatgtcgc gacttatagc ggttccagga ttggtgtatc cgctcgtacg
      901 ggtagtgcgg gccttcaggc ccgcgtccgg gagcgcgaac aaaaatcgct tcagccgctg
      961 aggtcttcga cctcagcggc tgaagccaag aattgcgcag gcatcttaac gcgggcctga
     1021 aggcccgcac tacccgagcc acagccattc tggaaccgct ctagcgcctc aaagtgcagc
     1081 tcacaaccaa cggccgagca ttcgtcagct attacttcgt gttcaaccct tttggcggag
     1141 aattaggatt ctccgcgcca catccgcatg gcgaaactcc aggcgacgat cggtaacagc
     1201 atcgcgattc cagttgcact ggccactact tggggtgcaa taccgcggtc aatggcttgt
     1261 ccggcggtcc acgaagcgat ggcgaccatc agcatggcaa ttcccaaatc ggcggaaaac
     1321 acacgcccgc ggaagcggtc ctcggtattc aggtgaagca gggtggtgga gaatacccag
     1381 acagtggacg cgcccgtgtg agccagcatg accactgcac acgcgaccca cagattatga
     1441 gtaagcccga gggacacgta gccaacgccg ccaagtagaa agcctaaaaa tatgccgcta
     1501 cgcagtcgct gctgccgacg tccggcccaa tgcgacgcca gcagcggtcc gacaagcgcc
     1561 cccagtccgc gcgcacccat cagcaggctc atgccaaaca gcgcggcgcg ctcgcgcgag
     1621 atggcaaaag gcaacgacag gacccgctgg ccgagcacgg gaaacaacac ccacgctgta
     1681 cccagaatgc cgacgccgcc tttcagggaa accatggcca gcagtttccg attgtgcgag
     1741 atataacgga ggccggcgac aatgggggaa tagtcgaaga gctcgcgaat tttgaccggg
     1801 cccgccgccg cgaggtgggc ctcgtggaaa tgcatacggc cgatcagcac ggccgacacc
     1861 aggaacgaca gcgcgttcaa tacgaatacg gcatcgcgac caaacagtgc cgctaccgct
     1921 ccgcccatca aggcccctat cgccaggtta aacgaccatg tcgcggacgc gagggtattc
     1981 gctacgatca cgtcttcttc gacggtaatg ctgggaatga tggagttccg agcgggttcg
     2041 aagaacgccg ccatcaccgt ctccagcagg agtaagggat aaatcaccca gacaaatcgt
     2101 gccgagcgca ccagcagcat ggaaccgacg atcagcatgc gcatgaggtc ggcgaagatc
     2161 atcacccgcc gccggctcag gcggtcgttg atcgctcctg ccagcggtcc cacgaaggtt
     2221 tgcggcagga cttgcagcac cagggccagc gccacggagg aagcgcgccc ggtaagatcc
     2281 agcagcaagc tgtagatggc aagggtgtag aaccagtcgc cgagttcgct gacgatctgc
     2341 gcaaaccata gtttgcgaaa gttatggttc tcgcgtgtga gacgaaggta ttcggagagg
     2401 gatacggagg aggccatgaa ccggaattag cctaacagag acggcgggtg acatggaggt
     2461 gccaggggtc aatacctgtt acactcttgc gcacgcaata tggcaaacca ggctgaagct
     2521 cttcgcaccc ggctgtacga caatcccgag ggcaa
//
LOCUS       DASRKK010000754         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_960512, whole genome shotgun sequence.
ACCESSION   DASRKK010000754 DASRKK010000000
VERSION     DASRKK010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960512"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            755..1309
                     /locus_tag="VFC15_18170"
     CDS             755..1309
                     /locus_tag="VFC15_18170"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZM12141.1"
                     /translation="MMAFSTRFSYAFRCFFSLLFSGTIPPDIGRAMASATGASAPNEP
                     AAAKPKPSENPPVESFDRAVQMLALLQRDGRLIDFLSEDVSPYPDSQLGAAVRTIHAS
                     CRQVLDRYLKLEPVISSEEDQPVTVPAGFDPATVKLLGNVVGQPPVKGLLRHRGWRVT
                     EVKLPSLPQGAGREILAPAEVEIP"
     gene            1375..>2028
                     /locus_tag="VFC15_18175"
     CDS             1375..>2028
                     /locus_tag="VFC15_18175"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZM12142.1"
                     /translation="MDSEFIVGIDLGTTNSALSYCAVADEGGQITGEKIPQLANPHEV
                     ADRTLLPSFLYVPGEVDFPAGSLGLPWDDAPKFVIGELARKRGAENPARLVASAKSWL
                     SHAAVNRTASILPWQAPDEVTKVSPVEASAQYLEHMRSAWDSQHASGGSGSRLGQQDV
                     LLTVPASFDEEARELTLRAAEQAGLERVTLLEEPQAAFYAWLESQGDNWRRNIKVGDL
                     "
BASE COUNT          409 a          568 c          600 g          451 t
ORIGIN      
        1 cgtcgagagg gagctgccaa tatcaagtct gcaagcaact gacttgccct tggctgtacc
       61 aactggacgc gcaaacacct cagaccaccg atttatattc ggcccctaaa tcgacagtct
      121 atatttgtgc aggaaaggat aaacccgcat gtgagccacg gtgatggttg catttctttt
      181 ggtgtccttt ttcggcctgt cccggtgcgc tgcacgtcgc tacgggagca tcaactcact
      241 cggagccgga tgagggcgtg ccctccccta ccggtgaaac tacggtgcaa agtagctgga
      301 gatgtcgagg atcagctggg tgatccccga cgcataggca tcgacttttc cgtttgtgct
      361 gggcacgatg gccatgttgt tggtgattga gccctccaag gcaccatcac gcccattaag
      421 ccttggaagc atggtcggcc atcgaaagtt ggcccgaaac cggcttgtag gaggttacta
      481 acgataagtc ggccagggtc gacgaatgtg tgcgcttctg ccggtaagtg gcctcttcgg
      541 aattgccttc gcaacagagt cgatatctca tataaaatta aatcgattcg taaattcttt
      601 cggtccagcc ccgtgtttgc agatctggga aatctgcctc gcaggcgtga taacaaagtc
      661 cgccaacagt aatgcgaacg agggcttccg cgttaaacca aaggctgggt gaccgcagcc
      721 tcctctcaag acatcccgta tggagccaaa acctatgatg gcgttctcaa cccgtttctc
      781 ttacgcattt cgttgcttct tctcgctctt gtttagcgga actatcccac cggacatcgg
      841 gcgcgcgatg gccagcgcga ctggcgccag tgcgcctaac gaaccagcgg ccgcaaagcc
      901 caagcccagc gaaaatcctc cggtcgagtc tttcgaccgg gcggtgcaga tgctcgcctt
      961 gttgcagcgg gacggccggt tgatcgattt cctctccgaa gatgtctcac cgtatccgga
     1021 cagccagctt ggggcggcgg tgcgaacgat tcacgcttct tgccggcagg tgcttgatcg
     1081 ctacctgaag ctcgagccgg tcatttcctc cgaggaagac cagcccgtga ctgtgcccgc
     1141 cggcttcgat cccgccacag tgaaattgct ggggaacgta gtcggtcagc ctccggtcaa
     1201 gggattgctg cgtcatcgag gttggcgggt cacggaagtg aagttgccgt ccctgccaca
     1261 gggcgcgggg cgtgagattc tggctccggc agaagtcgag attccttagg tattgtcccc
     1321 atacttttca tcacattttg ttttggataa cacgattgtt tgacggaggt tcgattggat
     1381 tcagaattca tcgttggcat cgatttaggc acgacgaatt ccgcactatc gtactgtgcg
     1441 gttgcggatg aaggtgggca gatcacaggc gagaagatcc cgcagcttgc aaatccgcat
     1501 gaagttgccg accgcacgct tctgccttca tttctctatg ttccggggga agtcgatttt
     1561 cctgcgggaa gtctcggctt gccgtgggat gacgcgccga agttcgtcat cggcgagctg
     1621 gcccgcaaac gcggagctga aaatcctgcg cggctggttg cgtcggcgaa gtcatggctg
     1681 tcgcacgctg cggtaaatcg aaccgcctcg attctgccgt ggcaggcgcc agacgaagtt
     1741 accaaggtct ctccggtgga agcctctgcg caatacctgg agcacatgcg ctcggcgtgg
     1801 gactcccagc acgcaagcgg tggctccggt tcgcgcctgg ggcagcagga cgtgttgctc
     1861 acggtgccgg cgtcgtttga tgaagaagcc cgtgagttga cgctgcgcgc ggccgaacaa
     1921 gctggcctcg agcgtgtgac cctactcgaa gaaccgcaag cggcgttcta tgcgtggctc
     1981 gagagccagg gagacaactg gcgcaggaac atcaaggtcg gcgacctg
//
LOCUS       DASRKK010000755         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_863042, whole genome shotgun sequence.
ACCESSION   DASRKK010000755 DASRKK010000000
VERSION     DASRKK010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863042"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..506
                     /locus_tag="VFC15_18180"
     CDS             255..506
                     /locus_tag="VFC15_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12143.1"
                     /translation="MANKKPHSVVGMIGEAVGDVVDAASVAATGSQLGVIELAAEEEI
                     SPPPAKRKRKAKVARKTKKVVMKTKKKTKKVRGVKRPHR"
     gene            complement(658..885)
                     /locus_tag="VFC15_18185"
     CDS             complement(658..885)
                     /locus_tag="VFC15_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12144.1"
                     /translation="MLLIDISLSRYDDLIGILRGSELKSQPDSTKLLVRLWVPKTLVV
                     NPKTRQRLLFGHLNAPRFTAARALRYFDVAR"
     gene            875..1897
                     /locus_tag="VFC15_18190"
     CDS             875..1897
                     /locus_tag="VFC15_18190"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZM12145.1"
                     /translation="MSSMTRRKFMRTTSAAASGVALFGILTRRGDAAEFVYRYANNNV
                     IAHPMNIRLREAADKIKEESGGRMEIQLFPNSQLGGDSDMLSQLRTGAIQMFNLSGLI
                     LATYVPVASINGIGFAFKDYGQVWPAMDGALGAFVRSAIAKAGLYAFNNMWDNGYRQI
                     TSSTHPINTPDDIKGFKIRVPVSPLWTSMFKALGASPTSININEVYSALQTKIVEGQE
                     NPLSLINLFKFYEVQKYVSFTNHMWDGFWTLANAKAWSDLPKELQEIVARNINGAALK
                     EREDVRQLNDSLQGELTQKGMVFNKTDADKFRATLRTAGFYAEWKGKYGAEAWAALEK
                     QVGSLS"
     gene            1901..>2814
                     /locus_tag="VFC15_18195"
     CDS             1901..>2814
                     /locus_tag="VFC15_18195"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZM12146.1"
                     /translation="MAGSDSIEDLASEQAIATAAQPSDRPARFTHLDKVLAWATEVPA
                     ALLVAVEILVLLVGVISRYVFNRPLTWSDELASILFLWLAMIGSVIALRRSEHMRLAF
                     LVGLAPPRWRRFLETFATAVVAVFLIGMIAPARDYVEVQWFITTPALEIHDGYRVAAI
                     GVGATLMLVIVLARLVERASFAEAAGTLAAILVIAAGLWFAHPTLLALGNLNLLIFFV
                     GMVAFCVALGVPIAFAFGISTMLYLALTTHVPLTIIASRTDEGTSNLILLSVPLFVFL
                     GLLIEMAGLARVLVDFMAALIGHVRGGLS"
BASE COUNT          612 a          815 c          791 g          596 t
ORIGIN      
        1 ctccgaaact gacagaaaac ttagacgcct gtttgagaaa tccttctacc tcagcagttg
       61 cctttcgttc atctcgcatt gtcgcagcct gtggcacaac cgggccacat ttaactgatg
      121 cacggcatgg gccaccgcga gggggcgctt caaatgacca agtaagagcc cctggcgcgc
      181 tcttgggtcc gccagtacgc tattcgcgtc catggctcgt gactcgatac ttttgaacaa
      241 gtgaggttgg ggatatggca aataaaaagc cgcattcagt tgtggggatg atcggtgaag
      301 cggttggcga cgttgtggat gccgcaagcg tagcggccac cggctcacag ctgggcgtta
      361 ttgaattggc ggcagaagag gaaataagcc cacccccggc caagcgtaag cggaaggcaa
      421 aggtggcccg aaagacaaag aaggtcgtaa tgaaaaccaa gaaaaagact aaaaaggttc
      481 gtggcgtaaa acgtccgcat cgctgacaaa cgtctccttc ggccattcat gcatttaagg
      541 tctgctgaat ggaccgctgc cctccgaagc tgacaggaag cttggccgcc ggttcaagaa
      601 atccttgtaa ttcagcagtc gccgctcatt cctcacgctt ggccacagcc agtggcacta
      661 ccgggccaca tcaaagtaac ggagggcacg ggcagctgtg aagcggggcg cgttcaaatg
      721 accaaataag agtcgctggc gcgttttagg gttcaccact aaggttttcg gtacccacag
      781 ccgcaccaga agtttggttg aatccggctg tgattttagt tcggaaccac gtaaaatacc
      841 aattaaatca tcatagcggg agagggaaat gtcgatgagc agcatgacac gccggaaatt
      901 catgcggaca acgagcgcag cagcgagcgg tgtcgcctta ttcggtattc tgaccaggcg
      961 cggcgatgcc gctgagttcg tctaccgtta tgcaaacaac aacgtaatcg cccaccccat
     1021 gaacattcgg ctgcgcgaag cagccgacaa gatcaaggaa gagtcgggcg gtcgaatgga
     1081 gatccagctc tttcccaaca gccaacttgg cggcgactct gacatgctgt cgcaattgcg
     1141 caccggcgcc attcagatgt tcaatctctc cgggttgatc ctcgcaactt atgtgccggt
     1201 ggcctcaatc aatggcatcg gcttcgcatt caaggactat ggccaggtgt ggccggccat
     1261 ggacggcgcg ctcggcgcct tcgtgcgtag cgccattgca aaggccggcc tctatgcctt
     1321 caacaacatg tgggataacg gctatcggca gatcaccagc agcacacatc cgatcaacac
     1381 gcccgacgat atcaaaggat tcaagattcg cgtgccggtg agcccacttt ggacgtccat
     1441 gttcaaggcg ctcggcgcct cgccgaccag catcaatatc aacgaggtct attcggcgct
     1501 gcagaccaag atcgttgaag gccaggagaa cccgctctcg ctcatcaacc tgttcaagtt
     1561 ctatgaggtg cagaaatatg tttccttcac taaccacatg tgggacggct tctggacact
     1621 cgccaacgct aaagcgtggt ccgacctgcc gaaggaattg caagagatcg tagcccggaa
     1681 catcaacggt gcggctctga aggaacggga agatgttcgc cagctcaacg attcattgca
     1741 gggcgagctg acgcaaaaag ggatggtctt caacaagacc gacgccgata aattccgcgc
     1801 tacactgcgg accgctggat tttatgccga atggaagggt aaatacgggg cagaagcctg
     1861 ggctgcgctg gagaagcaag ttggcagctt gagctgaccc gtggcgggca gcgacagcat
     1921 tgaagatctc gcctccgagc aggcgatcgc aactgccgca cagccatcgg accgcccggc
     1981 ccgtttcacg cacctcgata aggtgctcgc gtgggcaaca gaggtgcccg cagccctgct
     2041 cgtcgcggtc gaaatcctcg tcctgctagt cggtgtcatt tcgcgctacg tgttcaatcg
     2101 tccgctcaca tggtcggacg agcttgcctc catcctcttc ctatggctgg ccatgatcgg
     2161 ctcggtgatc gcgctgcgcc gctccgagca catgcgactc gcttttctag tcggactagc
     2221 cccgccacgc tggcgacgct ttctcgaaac ctttgcaacc gcggtggtcg cagtcttttt
     2281 aatcggaatg attgcgccag cccgcgacta cgttgaagtc caatggttca tcaccacgcc
     2341 ggctcttgaa atccacgatg gctatcgcgt ggccgccatc ggcgtgggcg ctacgctgat
     2401 gctcgtaatc gtgctggcgc gcttggtcga gcgtgcgagc tttgcggagg ccgccggaac
     2461 gctcgccgca attctcgtga tcgcagcagg gctctggttt gcccacccca ccttacttgc
     2521 cctcggcaat cttaatctgc tgatcttctt cgtcggcatg gtcgcgttct gcgttgcgct
     2581 cggtgttccg atcgcgtttg cgtttggaat ttcaaccatg ttgtatctcg cgctaacaac
     2641 gcacgtgcca ctcaccatca ttgcgagccg gaccgacgaa ggcacgtcga acctaatcct
     2701 gttatcggtt cccctgttcg tgttccttgg cctgctcatc gagatggcgg gcctcgcgcg
     2761 cgtgctggta gatttcatgg ccgcgctcat cggccacgtg cgcggtggcc tctc
//
LOCUS       DASRKK010000756         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_275701, whole genome shotgun sequence.
ACCESSION   DASRKK010000756 DASRKK010000000
VERSION     DASRKK010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275701"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1148)
                     /locus_tag="VFC15_18200"
     CDS             complement(57..1148)
                     /locus_tag="VFC15_18200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM12147.1"
                     /translation="MKIAIVGTRGIPNRYGGFEQFAEKVSSTFADRGHEVTVYCRKPF
                     TTTDDLYDRRVRRVILPSIHQKHMDTWSNGLISTAHAAFSDCDVVLLCNVANSPFAWF
                     PRLFGKPVVLNVDGLDRQRGKWNALGQAALHVCEWLSTVTPTRVVTDAQPIQDYYRTR
                     YRKLSTLIGYGSDIPPGEHKLDGFNLQPGRYALYVSRLEPENNPDLVLNAWRKVRTDW
                     PLVMLGDNRYSDAYLERLKQLADDRVIFPGAIYGDGYWALQKNAGIFVFACEVGGVHP
                     ALIEAMAAENPVLYLDSPENNETAGDAAIRFSKSADDLAAKLQSLLDDPAARQQWAAR
                     AAARAHQLYRWDEVAERYEKLFDEVLRRR"
     gene            complement(1145..2209)
                     /locus_tag="VFC15_18205"
     CDS             complement(1145..2209)
                     /locus_tag="VFC15_18205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM12148.1"
                     /translation="MVSVLVVTWNSARYLQQCFASLDRQNYPDLEVVVIDNASTDGTR
                     EFLQSREPLWHVIYNDRNVGFAAGQNQAIRAAKGEWLLCLNPDVLLADDFVNRLVEVG
                     SAHPEVGSICGKLLRWDPDHDPHQTRTIDSTGIYFTRNMRHLDRGAEELDSGQYDRLQ
                     YVFGATGAAALFRRSFVEQVSMDGEFFDEDFFSYREDADLAWRAQVMGWRCLYTPTAV
                     AWHVRRVTPERREQLPLVINWHSVKNRFLMRGKNASGWLCRRLFWPVAWRDLTIVGYA
                     LLRDRRLLSAIAYPWRVRDSVRRKRAIIQSRRKVSDRHLIWWFSDTPRATYADVTTSA
                     IPVAASPAAATDTSISGDRP"
     gene            complement(2216..3652)
                     /locus_tag="VFC15_18210"
     CDS             complement(2216..3652)
                     /locus_tag="VFC15_18210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZM12149.1"
                     /translation="MFDRRSKLLSTVTLLQDLAVTAVAFAVAHLLRTFLVHFEFFSRH
                     LPGIYPFDHYVPLMLAFLLAWAVVGYTSQFYRDLEMSSPIQLVLNLLNQLAIVLVIIY
                     AGLYLFRRDDISRTYLLLVGIVDFVLLLIGRYISYNGIALMRKRLKRYHYFLIVGCGA
                     RAREMATLIEESRGMGLRLVGFVDPNSEGSNLGDRFGHYEVFPPSVLEQVLQDRVIDE
                     VVFAVNMQELARLEPLMAHCANVGVKIRVQLEFLPEAYSRIYLENFHDVPLLSLSSAP
                     ESELLLFFKRVFDVVLSAVSLVLLAPCLLAIAAAIRITSPGEVLFRQTRCGLGGRRFT
                     LYKFRSMVNNAEQLRAELHQLNELEGPVFKISDDPRITPVGRLLRRFSLDELPQMWNI
                     LRGDMSFVGPRPAIPDEVEKYEAWQRRRLRMRPGLTCIWVIEGRSDIEFHRWIQLDLA
                     YIDNWSLWLDAKIFLRTIPIVISGRGAY"
BASE COUNT          817 a         1072 c         1141 g          689 t
ORIGIN      
        1 ccctgtgggc gatcgggttg ggtggacgcg cggcccaata cccatcaaag taggcgtcaa
       61 cgccggcgca ggacttcgtc gaacagcttc tcgtatcgtt ccgctacttc gtcccagcga
      121 taaagctggt gggcgcgcgc agctgcccgg gcagcccatt gctgacgcgc ggcaggatca
      181 tcaagcaagg attgcaactt cgcggccaga tcgtcggctg acttgctgaa tcgaattgcg
      241 gcgtcacctg cagtctcatt gttttccggc gaatcaagat acagcacggg attctccgcc
      301 gccatggcct cgatgagcgc gggatgcacg ccacctactt cgcaagcgaa cacaaagatg
      361 ccggcgttct tctgcagtgc ccagtaaccg tcgccataaa tcgctccggg aaagatcacg
      421 cgatcgtcgg ccaattgctt gagacgctcc agataagcgt cgctgtagcg attgtcgccg
      481 agcatcacca gcggccagtc ggtgcgcacc ttgcgccagg cattgagcac gaggtcggga
      541 ttgttctccg gctcaagacg gctgacgtac agcgcatagc gtccaggttg caagttgaat
      601 ccgtcaagtt tatgttcgcc gggcggaata tcggagccgt atccgatcag tgtcgaaagc
      661 ttgcgatatc gcgtgcggta gtaatcctga atgggctggg cgtcggtcac cacgcgagtg
      721 ggcgtgaccg tggacaacca ttcgcagaca tgaagcgcgg cctgcccaag ggcgttccat
      781 ttgccacgct ggcggtcgag tccatcgacg ttgagcacca ccggcttgcc gaacaatcgc
      841 gggaaccatg cgaacggact gttggcaaca ttgcaaagca gcacaacatc gcagtcgcta
      901 aaggccgcgt gcgccgtgga aatcaggccg tttgaccaag tgtccatatg tttctgatgg
      961 atgctgggca ggatcacgcg gcgcacgcgg cgatcgtata ggtcgtccgt cgtggtgaac
     1021 ggcttgcggc aatagacggt gacctcgtgt ccacggtccg cgaaggtaga actgaccttc
     1081 tcggcgaact gctcgaatcc gccgtaacga ttgggaatgc ctcgtgtgcc gacaatcgca
     1141 atcttcaagg ccggtccccg gagatggagg tatcggtagc agcggccggc gacgcagcaa
     1201 ccggaatcgc cgaagtggtc acgtcggcgt aggtggcgcg tggggtgtca ctgaaccacc
     1261 aaatcaaatg acggtctgaa accttgcggc gggattggat gatggcacgt ttgcggcgga
     1321 cgctatcgcg cactctccag gggtaggcaa tcgcagacag caatcgacgg tcacgcagca
     1381 aggcatagcc aacgatcgtc aggtcgcgcc acgccacggg ccagaacagc ctgcggcata
     1441 gccagccgga cgcgttcttg ccgcgcatga gaaagcgatt ctttaccgaa tgccaattga
     1501 tgacgagcgg caattgttcg cgccgctcgg gggtaacgcg ccgcacgtgc caggccactg
     1561 ctgtaggagt gtacagacat ctccagccca tgacctgagc tcgccacgct aggtcggcgt
     1621 cctcgcgata ggaaaagaag tcttcgtcga agaactcgcc atccattgag acttgctcaa
     1681 cgaacgagcg ccgaaacaag gccgccgcgc cggtcgctcc gaacacatat tgaaggcgat
     1741 catactgacc gctgtcgagt tcttcagcgc cgcgatcaag gtggcgcatg ttgcgggtga
     1801 agtagatgcc ggtggaatcg atagttcgag tctggtgggg atcgtggtcc gggtcccagc
     1861 ggagcagctt gccgcagatc gaaccgacct cgggatgggc ggagccaact tcaaccagcc
     1921 gatttacaaa atcgtcggcc agtaacacgt cgggattcag gcagagcagc cattccccct
     1981 tcgcagcccg gatggcctgg ttttgtccgg cggcaaaacc cacattgcgg tcgttgtaga
     2041 ttacgtgcca cagcggctcc cgcgactgca ggaattcgcg cgtgccgtcg gtggaagcgt
     2101 tgtcgatgac gacgacttcc aggtcagggt aattctgccg atcgagcgac gcgaaacact
     2161 gctggaggta tcgagccgaa ttccaggtga cgacgaggac tgaaaccatc agcccctagt
     2221 atgccccccg gcctgaaatg acgatcggaa tggtgcggag gaagatcttg gcatcgagcc
     2281 acagcgacca gttgtcgata taggcgaggt cgagctggat ccagcgatgg aactcaatat
     2341 cgctgcggcc ctcgatgacc cagatgcagg tgaggcccgg acgcatgcgc aggcggcggc
     2401 gctgccaggc ctcgtacttt tcaacctcat cgggaatcgc cggacgaggg cccacgaacg
     2461 acatgtcgcc acgcaggatg ttccacatct gcggcagctc atcgaggctg aagcggcgca
     2521 ggagccggcc tacgggcgtg atgcgcggat catcgctgat cttgaacacc ggcccttcga
     2581 gttcgttaag ctggtgaagt tcggcgcgca attgttctgc gttgttcacc atcgagcgga
     2641 atttatagag ggtaaaccta cggccaccaa ggccacagcg ggtctgccgg aagagcacct
     2701 cgcccggcga agtgatgcgg atcgcggccg caattgcaag cagacatgga gccagcagca
     2761 ccagtgacac tgcagaaagc acgacgtcga agacccgctt gaagaacagg agcaattcgc
     2821 tctccggcgc gctggagagg ctgagtagcg ggacgtcgtg gaagttctcc aggtagatgc
     2881 gcgaataggc ctccggcaga aactccagtt gcacccggat tttcacgccc acattcgcgc
     2941 agtgcgccat cagtggctcc aggcgcgcaa gttcctgcat gttgaccgca aacacgacct
     3001 cgtcaatgac ccggtcctgc agaacctgtt ccaacacact tggcgggaaa acctcgtagt
     3061 ggccgaacct gtcgccaagg ttcgagcctt cgctgttagg gtccacgaaa cccaccagcc
     3121 ggagtcccat accgcggctt tcttcgataa gggtcgccat ctcccgcgcc cgggccccgc
     3181 atcccacgat caggaagtag tggtagcgct tgaggcgttt tcgcatcagc gcgataccgt
     3241 tgtaagaaat gtaacgtccg atcagcagga gtacgaagtc cacgatgccg accagcagta
     3301 agtaggtgcg gctgatgtcg tctcggcgga agagataaag tccggcgtaa atgatgacca
     3361 gcacgatggc gagctggttc agcagattta gaacgagctg aatcgggctc gacatctcca
     3421 ggtcgcgata gaactgggac gtgtagccca ccacggccca tgccagcagg aacgccagca
     3481 taagcggcac gtagtggtcg aagggataaa tgcccggcag atggcggctg aagaactcga
     3541 aatgcaccag gaaagtgcgc agcaggtgag ctaccgcgaa ggccacagcc gtcactgcca
     3601 agtcttgcag cagtgtcacg gtgctgagta acttggaacg tcgatcgaac acgttttacc
     3661 aggtcctggt ttttctattt cgtcttagtc gaataggcgt tctcgacaaa gagcgtctg
//
LOCUS       DASRKK010000757         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1059468, whole genome shotgun sequence.
ACCESSION   DASRKK010000757 DASRKK010000000
VERSION     DASRKK010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059468"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            570..1604
                     /locus_tag="VFC15_18215"
     CDS             570..1604
                     /locus_tag="VFC15_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZM12150.1"
                     /translation="MDHFAGLDVSVKETSVCIVDDAGRIVREVKVASEPEALLPVLTS
                     GAYHFKRIGLEAGPLSQWLFSALAEAGLPVICVETRHMRAVLQAQINKTDRNDARGIA
                     QMMRVGLYRPVHVKTLRSQKLRMLLTHRKLLQSKAIAIDNDLRGTLRNFGLKVGMVGT
                     VKFEARIQELVANLPDLAALVEPMLIVRRVLREQLGILHRRLLAIVRDDDVCRRLMTV
                     PGVGPVVALTYRATVDVPARFKNSKAVGAVFGLTPSKYQSGEINRTGGISRCGDEMMR
                     VMLYEAAQVMLTRTNKWSWLKAWAMKIARRRGMKKAIVALARRLAVIMHRIWVDGTEF
                     RWTREETAAA"
BASE COUNT          409 a          570 c          669 g          428 t
ORIGIN      
        1 caatatgcca attctgaagg gcatcgtaac ggtttaaatg gggatattgg aaggcctcgc
       61 tgatgtccgc tttgggtcat gtgtggacgg cgccttggca agaactttct gacgttactg
      121 cagcattggt cgggtgcggt catgtgtccg gcctgtttgt gcggccggtc tggccgctgg
      181 ccataatgct ctgcatgctc tgcgcggatc gggtcccaat cgttagcacg cactttgaag
      241 tgcgatgacc caaacgggtt ctcccgatcc ttgcaacgac cttgtctgca ttaagtcgtc
      301 atgtcctcgc caattccttc aaacttcagc tccatatcat ttctgccgct gcccggcagt
      361 ggtctcacct ccctgatttg ctctcacttc agcgcaacca catcatagca cttcgatgcc
      421 gcagagcggg cgccgtccac agcaccatta gatggggtaa tcggccggca gcttgtggat
      481 agctgaagat tttgggtgct gcgcttcagg ctgatggtga atacgaggtc cgtggtcacg
      541 gcctcaagca tcagaaggag aagcgcagca tggaccattt tgctggactg gacgtgtcag
      601 tcaaggagac gagcgtctgc attgtggatg acgcaggcag gatcgttcgg gaagtgaagg
      661 tagcgagtga gccggaagct ttgctgccgg ttctgaccag cggcgcctac cacttcaagc
      721 ggatcggact ggaagccggg ccgctgtcgc aatggctatt cagcgcactg gccgaggcgg
      781 gcttgccggt gatctgcgtc gagacgcggc acatgcgggc ggtgttgcag gcgcagatca
      841 acaagacgga ccgcaacgat gcgcgtggta tcgcgcagat gatgcgggtg ggactttacc
      901 gcccggttca tgtgaagacg ctgcgcagtc agaaactgcg gatgctgctg acgcatcgca
      961 agctgctgca gtcgaaggcc atcgctatcg acaacgatct gcgcggtact ttgcgcaact
     1021 tcggcctcaa ggtcggcatg gtcgggacgg tgaagttcga ggcgcgtatc caggagctcg
     1081 tcgcgaacct acccgacctg gcggcgctgg ttgaaccgat gctcattgtc cggcgggtgc
     1141 ttcgcgagca gctcggcatc ctgcaccgcc gcttgctggc catcgtgcgg gacgatgatg
     1201 tgtgccggcg cctgatgacg gtccccggtg tcggcccggt ggtggcgctg acctatcgcg
     1261 ccaccgtcga cgtgcccgcc cgcttcaaga actccaaggc ggtcggggcg gtgtttggac
     1321 tgacaccgtc caagtatcaa tcgggcgaga tcaaccgaac cggcgggata tcgcggtgcg
     1381 gagacgagat gatgcgggtg atgctctacg aagcagccca ggtcatgttg acccgcacca
     1441 acaagtggtc ctggctcaag gcctgggcca tgaagatcgc caggcgccgc ggcatgaaga
     1501 aggcgatcgt ggcgctggcg cgccggttgg ccgtaatcat gcaccgcata tgggttgacg
     1561 gcaccgagtt ccgctggacc cgggaagaaa cagcagcagc atgagagcag tccaggatca
     1621 catgcgatag gagaacactc gagctccacc cagtggtgga atgatgtccc tcgcgggacg
     1681 atggatgagg tgagttcgtt tggtcgcttg gacctgccgt tcatagaacg gcaaggccga
     1741 ccgttagatt gttccacctc gttctcgact cccatgatgg aagggccctg gtgccgattc
     1801 cgaagagaag cacgagcccg tgagcgacat cgacactgca gtcgtggata gtctgaaagt
     1861 gcttgacccc ggacggccga ttagagaagc gaccaaaatg gtgcggcgcg gcgaatgacg
     1921 cgaagtgcca ataggcgaca ttgggcgctt ggtttgaaat gaaaaaggcc gccaattgag
     1981 gcggcctcca attcattgtg gttgcgttag gcagcgcggg ccggcgcagc caatgggggc
     2041 gtgccatctt ttattgcgct tcttatcatc ttacgc
//
LOCUS       DASRKK010000758         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_276355, whole genome shotgun sequence.
ACCESSION   DASRKK010000758 DASRKK010000000
VERSION     DASRKK010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276355"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFC15_18220"
     CDS             <1..236
                     /locus_tag="VFC15_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12151.1"
                     /translation="GDRAACSAPRIKPLAQLDDVHSILRLSYETLKGLISLASRGVAK
                     LTRFETRLRNPRQTGGVLKMGERGYNCKHAGRS"
     gene            complement(1207..1893)
                     /locus_tag="VFC15_18225"
     CDS             complement(1207..1893)
                     /locus_tag="VFC15_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12152.1"
                     /translation="MSHDIGHDRTLKPMQQLGVLVAVVLLGGQALGQGTTSGAPAKPP
                     DAAKNPWAYNLTVDGYIIPHSQSYVDPVFTADRNWLHLEARYNYENLQTGSLWAGYNF
                     SAGKKLVLDVTPMIGGVFGRTNGIAPGCEASLTYKKIELSISNEYVFDTTHKSGNFYY
                     SWPQLTYSPVDWFRVGLVAQRTKAFHTSLDTQRGFLVGVSHKKWEFTTYIFNAGFTDP
                     TIVLEGGVSF"
     gene            complement(1890..2483)
                     /locus_tag="VFC15_18230"
     CDS             complement(1890..2483)
                     /locus_tag="VFC15_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12153.1"
                     /translation="MVLDFTLIGGLTAGAGKHGVRHLLIAAIGCNVAWGIIDGALYVA
                     GNLTARRQRFQLLSKIRNAENESAAFSAISGQLDSLLEPATSAEDRTRICQAFLPALS
                     RIRLPEAGLVKDDVYGMAAIFWIDLASVFPAVIPFLIFSSEPRFALRVSNALLIASLF
                     AAGFMWGKYTGSNRYVAGGCAMLIGLALVGVAIALGG"
     gene            complement(2604..>2873)
                     /locus_tag="VFC15_18235"
     CDS             complement(2604..>2873)
                     /locus_tag="VFC15_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12154.1"
                     /translation="ALLGTRLSLPSVFFMGWFGPRGLASIVLGLVYLEQELHLPNESA
                     IRATVMLTVVLSIFAHGLSAMPGISIYARKIASLAADAPEHQDAR"
BASE COUNT          664 a          824 c          817 g          568 t
ORIGIN      
        1 gcggcgaccg tgcagcctgc tcagccccgc ggatcaaacc cttggctcaa ctcgacgacg
       61 tgcattcgat tttgagactg agttatgaga ccctgaaagg gctgatttca ttggcctcac
      121 ggggcgtcgc aaaacttacc cgttttgaga cacgtttacg aaacccgcgc caaacgggag
      181 gcgttttgaa aatgggggag cgcggataca attgcaagca tgccggaaga agctgagcaa
      241 ccaatggtcc ttgcctcggt tgacttgtat gactacggcc cagacatttc cctcgcagtg
      301 tcttggacgc gggcggctcg tgtcagaatg gacacggtgg aaaagctggc caagctgaag
      361 gcgtcagcag tcccggacct tctggatgca atcggaacct acgccatctt gatgcgtgga
      421 agcgcttcgg tcgaacttgg aattcatccg agccgtccga cctacataca gagcagaagc
      481 gagatgaaaa gggttaacga agaaattcgt aggcgtctac gaagcggttg gccaaacctt
      541 tgccacggag cggcgctgac gcttaccaaa atcggcggag atgcagtcga gccaacgatt
      601 gccgctgttc gcgagaatga ttggcttgcc gcgattgaat tggtttgggc gctaggtgag
      661 ataggtgaca aacgtgccgg cccccttctg cgccggatat tcgcatggtg gaatgtcgac
      721 caatttccag aggtcaagca aatggccaag cgtccaataa tgacggaact tcatcggacg
      781 agccctcgcg aaggctgcaa caccggttta gtgtgggtcg aagagaaaat gaattctacc
      841 gcaaagcctc gctaccgctc cgagcccgct ccaaacgagt cgaaccccgg acaccgctga
      901 aacggcatta gcgtaggtct cataaaggac cctttgtgag acggggaaag aacctgatta
      961 cgtgtgagat ggcgatttca caatacctat ctcagacata gcggtggatt gtgttatctg
     1021 ggcggcggac gttgagggca tagatttccc gcgagccggt gtcgcgggtc gcgggtaatc
     1081 actgggactc aatcaggcgc ggcaaacggc aggagcgcac acattcgtag gcgctggctt
     1141 ctgggtactg atcggcctgg ataccgatag gatcttgctg tcgcgtttgt gatgccgcac
     1201 tggccctcaa aaactgacgc ccccctcaag cactatcgtc ggatcggtaa agccggcatt
     1261 gaagatatag gtcgtaaatt cccacttctt atgggacacg cccaccaaaa acccgcgctg
     1321 cgtgtcgagg ctagtatgga aagcctttgt gcgttgcgcc accaggccta cgcgaaacca
     1381 gtccaccggc gagtacgtta gttgtggcca actgtagtag aagttcccag atttatgcgt
     1441 tgtatcgaat acgtattcat tcgagatcga cagctcaatc ttcttatagg tgagcgacgc
     1501 ctcgcaaccg ggcgcgatcc cgttggtcct cccgaaaacg ccaccgatca tcggcgtcac
     1561 gtccagcacc agcttcttgc cggcgctgaa gttgtagcct gcccacaacg atcccgtctg
     1621 caggttctcg tagttgtagc gcgcctccaa atgcagccaa ttgcggtctg ctgtgaaaac
     1681 cggatccacg taggactgac tgtgcggaat gatataacca tcgaccgtga gattgtaggc
     1741 ccacgggttc ttggcggcat caggaggctt agccggcgcg ccgctcgtcg ttccctggcc
     1801 caatgcctgg ccgcccagga ggacaactgc cacgagcacc ccgagttgtt gcatgggctt
     1861 cagagtacga tcgtgaccga tgtcgtggct catccgccca gcgcaatggc aacaccgacg
     1921 agcgccaagc cgatcagcat cgcgcagcct cccgccacat agcggttgga gcctgtgtat
     1981 tttccccaca tgaaccccgc ggcgaacagc gatgcgatca gcagagcatt cgaaactcga
     2041 agcgcgaagc ggggctcgga ggaaaaaatc aggaacggga tcaccgccgg gaataccgag
     2101 gccagatcga tccagaagat cgcggccatc ccgtagacat cgtccttgac gaggccagcc
     2161 tcaggcaatc ggattcgtga cagcgcgggc agaaacgcct gacaaatgcg ggtgcggtct
     2221 tccgcgctcg tcgcgggctc cagcagcgag tcgagctggc cactaatcgc cgagaatgcc
     2281 gcgctctcat tttcagcgtt cctgattttg ctaagcaact ggaaacgctg gcggcgagcc
     2341 gttaagttgc ccgccacgta cagtgcacca tcgatgatgc cccaggcgac gttacagccg
     2401 atagcggcaa tcagcaggtg ccgtactccg tgttttccag cgcccgccgt cagacccccg
     2461 atcagggtga agtcgagcac catgatcagt ccacacagga cttcttccaa tcggtctgcc
     2521 ggatccagat actttgtgac caaccgcgtc cttgaatgag aaaaggccaa ctcaatcctc
     2581 ccgaagtctt cgcctctaac ccctcatctt gcatcctggt gctctggggc gtcggcagca
     2641 agcgatgcga ttttgcgtgc gtagatgcta atgccgggca tggcactcag cccgtgggca
     2701 aagatgctga gcactaccgt cagcatcacg gtggctcgaa ttgcagattc gttagggaga
     2761 tgaagctcct gctctagata caccaatccc agaacaatcg acgcaagccc acgcgggcca
     2821 aaccatccca taaagaaaac cgagggtaga ctaaggcgtg tcccaaggag cgc
//
LOCUS       DASRKK010000759         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_570810, whole genome shotgun sequence.
ACCESSION   DASRKK010000759 DASRKK010000000
VERSION     DASRKK010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570810"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFC15_18240"
     CDS             complement(<1..186)
                     /locus_tag="VFC15_18240"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZM12155.1"
                     /translation="MDSRVVVVSGSAKGTVVRLTGSQFSVGRDSTNHLCLHDRAVSRR
                     HFSISETDAAFHLVDLDS"
     gene            complement(518..718)
                     /locus_tag="VFC15_18245"
     CDS             complement(518..718)
                     /locus_tag="VFC15_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12156.1"
                     /translation="MTFLLWCILLFCCWPLALFVLVLYPLVWLLLLPFRLLGFAVEGV
                     LELLKAIILLPARVLRGPRRAW"
     gene            complement(773..2167)
                     /locus_tag="VFC15_18250"
     CDS             complement(773..2167)
                     /locus_tag="VFC15_18250"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZM12157.1"
                     /translation="MIPGTEYVIEITYIVAAVLFILALKWLSSPVTARRGVWAGEVGM
                     VLAIAGTLLYRGIVDYKWIAIALVLGAIIGVPLGNVGMTAVPQRTALSHAFGALCVTL
                     VGTAEFYLRAPNVPPFLMSVLSLEVILGGLTVTGSLMAAGKLQEILPQRPITYKGQNF
                     VNLAMLAIAVAIAVRLVLHPEAMVLYPFMVGIALVFGVMMIVPIGGADMPTVISLLNS
                     YAGLSAAAMGFVLNSKLLIVAGALDGASGFILSVNMSKAMNRSFTNVLFGAFGQVQTS
                     GAAAVEARPVHSATPEEAAAILSAANKVVIVPGYGMAVAQAQHKVRELYDLLTKKGVD
                     VKFGIHPVAGRMPGHMNVLLAEADIPYDKLLDMEEVNADFPQTDVALVIGANDVTNPA
                     ARSDVGSPIYGMPILDVDKARTVMVIKRSMNPGFAGIDNPLYYLDNTLMLFGDAKAFV
                     GSIIRELSGGGGHG"
     gene            complement(2164..>2398)
                     /locus_tag="VFC15_18255"
     CDS             complement(2164..>2398)
                     /locus_tag="VFC15_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZM12158.1"
                     /translation="VIRRVSPLLHTPLMSLTNALDAIAVVGAIILAGEHKSTLATVLG
                     TIAIVAATSNAVGGFIITDRMLKMFKSSRTQKP"
BASE COUNT          560 a          729 c          685 g          424 t
ORIGIN      
        1 gctatccagg tcaaccagat ggaaggctgc gtcggtttcg ctgattgaga aatgcctccg
       61 cgacaccgcc cggtcgtgca ggcacaggtg gttggtggaa tcgcgcccca cggagaactg
      121 gctgccagtc agccgtacca ccgtgccttt tgccgagccc gacaccacca cgactctcga
      181 gtccacgaac cgcctccggg ctgctagttt agcccactcc tggcgaagtc acggaattaa
      241 tctgaaaaca gcatggagga taaatcgatg acggccggca attaccggat catcgcgctg
      301 caggctgaag gcgaaaggca catccgcccg aacgcggggg catcgggctt gccgagtcgg
      361 aacgcgagaa agaagtgaag atggggccgc cggtcttccc aacgcgcccg aaaccgtggt
      421 tgttgcaatg aagtagttgt agcctcgcga acgaagaaac ccgaagtggg agctccgggt
      481 ttcttcattt gatgtatgcc caatggaagg tcggttctta ccaggcgcgg cgcggcccgc
      541 gcaatacgcg cgccggaagc aggatgatgg ctttcagcaa ttccagcacg ccttcgacgg
      601 caaatcccaa aagccgaaac ggcaacagca gcaaccacac caacgggtac agaaccagca
      661 cgaacagcgc cagcggccag caacagaaca acaagatgca ccacagaagg aaggtcaacg
      721 taactcctcc tctctgtaaa tacgttacac ccgaccgcga agttccatgc cgctaaccat
      781 ggcctcctcc gccgctaagc tcccgtatga tcgagccgac aaacgccttc gcgtcaccga
      841 atagcatgag cgtgttgtcg agatagtaga ggggattgtc gattccggca aatcccggat
      901 tcatgctgcg cttgatgacc atgaccgtac gggccttgtc cacgtccaga atcggcatgc
      961 cgtagatcgg acttccaacg tcgctgcgag cggcaggatt ggtgacgtcg ttggctccga
     1021 tcacaagcgc aacatctgtc tgcggaaaat cggcattgac ttcctccatg tccagcaact
     1081 tgtcgtatgg gatgtccgcc tccgctaaca aaacgttcat gtgcccgggc atgcggcccg
     1141 cgaccgggtg aatgccgaat ttcacgtcca cgcccttctt ggtcaacaag tcatacagtt
     1201 cgcggacctt gtgctgcgcc tgcgccaccg ccatgccata acctggaacg attacgactt
     1261 tgttagcagc cgagaggatg gcggcagcct cttccggagt tgcactgtgt acgggccggg
     1321 cctcgaccgc ggcagcccct gacgtctgta cctgcccgaa cgctccgaac agaacattag
     1381 tgaacgatcg gttcatcgcc ttcgacatgt tgacagagag aatgaagccc gacgctccat
     1441 cgagcgcacc tgccacgatc aagagcttgc tattgagaac gaagcccatg gcggcggcgg
     1501 aaagtccggc gtaggaattc agcagcgaga tcacggtcgg catgtcagcg ccgccaatgg
     1561 ggacgatcat catcacaccg aacactagcg cgatccccac catgaacgga tacaggacca
     1621 tggcctcggg atgcagcacg agacgaacgg cgatggcgac tgcaatcgcc agcatcgcca
     1681 ggttgacaaa attctgcccc ttataggtga ttggccgctg cggcaagatt tcctgaagct
     1741 tgcccgcggc catcaagctg ccggtaacgg tgagaccgcc gaggatgact tccagcgaaa
     1801 gcaccgacat caggaacggg gggacattgg gggcgcgcaa gtagaactcc gcggttccca
     1861 ccagagttac gcaaagcgca ccgaaggcat gactcaatgc agttcgctgc ggaaccgcgg
     1921 tcattccgac attgccgagc gggacgccaa tgatcgcacc cagaaccagt gcgatggcga
     1981 tccacttgta gtccacgatg ccgcgataca gaagcgtgcc ggcaatcgcc agcaccattc
     2041 ccacttcgcc tgcccaaacc ccgcgccgcg cggtgactgg cgaactcagc cacttcaacg
     2101 cgaggatgaa gaggaccgca gcgacgatgt aagtgatctc aatgacgtat tcggttcctg
     2161 ggatcatggc ttttgtgtcc ggctggattt gaacatcttc agcatgcgat cggtaatgat
     2221 gaagccgccc acggcattgc tggtggcagc gacgatagca atcgtgccca gcacggtcgc
     2281 aagcgtgctc ttgtgctcac ccgcgagaat gatggcgccg acgaccgcga tcgcgtccaa
     2341 cgcgttggtc agcgacatca acggtgtgtg cagcaacggc gatacacggc gaatcacc
//
LOCUS       DASRKK010000760         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1452050, whole genome shotgun sequence.
ACCESSION   DASRKK010000760 DASRKK010000000
VERSION     DASRKK010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452050"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..977
                     /locus_tag="VFC15_18260"
     CDS             504..977
                     /locus_tag="VFC15_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12159.1"
                     /translation="MSRRLRFIPAILAILAFTGLIGCKPKPKTTPQVVRVLRNLNSPY
                     GSEMDLRIVEFQISNPRMQSGKPIVVQTVEMRDYPDLLKNHFGKDVNADIVILDAPSD
                     ADLNASVKADMANAVNICAAVKACPAEVPALVPSSDSADSKEAAQKFLDALQKHQ"
     gene            complement(1060..2271)
                     /locus_tag="VFC15_18265"
     CDS             complement(1060..2271)
                     /locus_tag="VFC15_18265"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HZM12160.1"
                     /translation="MAGTYYRVDINMAGASHYEIGRQYALQIKNNVPGYETRIDSFLQ
                     FMIGLMQDAQRHVVPPISPVIAFADLNARAHDLYPNIPQEYQQEIQGMQSVFSYATDR
                     IGDGRLSPNKLLVFELFGDIGRVNSCSASAAFGEGTVSGKTILGRSLEWKAGTNKYIS
                     AIHSVLILHNGSKSLVSFNALGGLAAISAFSSNKVFGAALDSEIYEEYPSTKGKRSYM
                     MDLRYALETATSLQDVANYLTGKDYAFNFNIFLADENSAAVFEDNTTEPFSGLRTATS
                     ALKLDIPDWDHPTAIATVNCFMLPGSADNCDLWDGNMPRWESFISRYEDELAQGKINI
                     DVMKRITAYPGPKSDGKAVDGAIFRYDEEPALQTIIVDMDSMETWVFFQPEGTPLTQP
                     NYLQVFSGNPF"
     gene            complement(2589..2891)
                     /locus_tag="VFC15_18270"
     CDS             complement(2589..2891)
                     /locus_tag="VFC15_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12161.1"
                     /translation="MQACLLDRVRFGQPDVLAIDDYGIRKGFAKIHKLAELPKPKELL
                     AYGERWRPYRSVASWYLWRAAEMKDLPMAGKQPKKAAAKRSTRAAPAKSKAKKSRK"
BASE COUNT          640 a          865 c          898 g          690 t
ORIGIN      
        1 gcaagacaac cagccagcgc catcttcccc ggcgcggcgt gtcgcgctct atccgcaaag
       61 atgccaactc atctttcagg tcccgcataa acggtgtcca actctccttg gacgatgcca
      121 ctagcatcgc attgttgccg cgagtttgga agagcatgac cgctgattac tacgttccgg
      181 gcgcggttta ggtcccgctt tttcccagca ctctaactga cagagatgat tgtcgccggg
      241 tcgtacgact ggcgctagtg tggaatgtca gcgctgcggg atgacaaatg tcatgcagca
      301 atcggacggc aatcggcttc gttatctccc ggcgagctga ctgctcgcgg acaaaagggg
      361 tccccgactc cccgctgcgc tcgtcgctca ggatgacagg tgaacaatgt tcattgcgaa
      421 actgacaact gacgacttgg ccactggcca ccgatccctc agcaacagga gacagagatg
      481 cctgctaacg gtatgatgtg acaatgtccc ggcgacttag gtttatccct gcaatcctgg
      541 caatactcgc gttcaccggc ttaatcgggt gcaagcccaa gccgaaaacc acgccccaag
      601 tggtgcgggt cttgcgtaat ctcaactccc cttacggcag cgaaatggac cttaggatcg
      661 tggagtttca aatcagcaat ccgcgcatgc agtccggcaa gccaatcgtg gtgcagacag
      721 tcgagatgcg cgactatccc gatctgctga agaaccattt cggcaaggac gttaatgccg
      781 acatcgtcat tctggatgct ccgtcggatg ccgacctgaa tgccagcgtg aaagccgaca
      841 tggcgaatgc ggtgaacatt tgcgccgccg tgaaggcttg tccggccgaa gttccggcgc
      901 tggttccatc ttcggattcg gcggatagca aggaagcggc gcagaagttt cttgacgctc
      961 tgcagaaaca ccagtagggg cgattttccc tggtgtgacg gtgcccatcc tagtgcctct
     1021 tttaggaagg atgggcaccc ggccgtgccc ggcttgcggt cagaacggat ttccgctgaa
     1081 cacctggagg tagttcggct gggttagggg agtgccttcc ggctgaaaga aaacccacgt
     1141 ttccatgctg tccatgtcta cgatgatcgt ctgtagtgcc ggctcctcgt cgtatctgaa
     1201 tatcgcgcca tccacggcct tgccatcgct tttcggaccc ggataggcgg taatacgctt
     1261 catcacgtcg atattgattt tgccttgcgc aagctcatct tcgtagcggc tgatgaaact
     1321 ttcccagcgt ggcatgttcc cgtcccacaa atcgcagttg tccgcgcttc cgggaagcat
     1381 gaagcagttt acggtggcga ttgcggtggg gtgatcccag tcgggaatgt ctaacttcag
     1441 ggccgaggtg gcggttcgca agccgctaaa tggctcagtc gtattgtctt cgaaaacggc
     1501 tgcgctattt tcgtccgcta ggaagatatt gaaattgaat gcgtaatctt tgccggtcag
     1561 gtagttggcc acgtcctgca ggctggttgc ggtttccaag gcatagcgga ggtccatcat
     1621 gtaggagcgt ttgcccttgg tcgacggata ctcttcatag atttcgctgt cgagagcggc
     1681 cccaaacact ttgttgctgc taaaggcgct gatcgccgcc aacccgccca gggcattaaa
     1741 gctgaccagc gatttgctgc cattgtgcaa tatcaggacc gaatggatcg cggagatgta
     1801 tttattggtg cccgccttcc attccaggct acggcctagt atggttttgc cgctcacggt
     1861 cccctcgcca aacgccgccg aggcggagca gctattcacc cggccgatgt cgccgaaaag
     1921 ctcaaaaacc aggagcttgt ttggggagag ccgaccgtcg ccgatgcgat cggtcgcgta
     1981 agaaaacact gactgcattc cttgaatttc ctgctgatat tcctgcggga tgttcgggta
     2041 caagtcatgg gcccgggcgt tgagatcggc gaaggcgata accggactga tcggaggcac
     2101 tacgtgacgt tgcgcatctt gcatcaggcc gatcataaac tgcaagaaag aatcgatgcg
     2161 cgtctcgtaa cccggtacgt tgttcttgat ctgcagtgcg tattgccggc caatttcgta
     2221 gtggctggca cccgccatgt tgatatccac acggtagtag gttccagcca ccttttgcat
     2281 ggtgacgaca ctggttgggt cgctggagcc gcacccttga aacgtggcca gtgagagtag
     2341 agcgatggcg aaaacgcaga ccttgatagt tttcacagca gacctcggtg gggggatccg
     2401 ctgcgcgttc gggcaggcgt aacgtcggat cgcccgacgg gagcgcgaag cgtcgttcgg
     2461 cgggcggcga ctctgagtga agggcaggcg cccgctgctc accttatcgg agggttgttg
     2521 ccgaaggtta attgggggcc ggcagatgtt tgcgggcgga cttggccatc aaagccaacc
     2581 taaccgcttc acttgcgcga tttcttagct ttagacttcg ccggcgcagc cctggtggac
     2641 cgctttgccg ccgccttttt tggttgctta ccagccatcg gaagatcctt catctcggcg
     2701 gcgcgccaca aataccagga cgcgacgcta cgatagggac gccagcgctc gccgtaggcc
     2761 agcaactctt tcggcttggg tagttcggcc agcttgtgaa ttttggcaaa gcccttgcgg
     2821 atgccgtagt cgtcaattgc cagcacgtca ggctggccga accgcactcg atcaagtaaa
     2881 caggcttgca acaggtatca cgatgttgct cagattgtgg ccaagcacac atggccagag
     2941 actatccgtg cactcgcgca tccacctgaa accaggagta tggcagtgat gacgagccgc
     3001 cagccgttga ggggaatgaa cacgggcaag accagcacgt cagtgtttgt cccctacccc
     3061 aggtcggcgt ctaagctcta agagaccgat ggc
//
LOCUS       DASRKK010000761         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1157554, whole genome shotgun sequence.
ACCESSION   DASRKK010000761 DASRKK010000000
VERSION     DASRKK010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157554"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFC15_18275"
     CDS             <1..213
                     /locus_tag="VFC15_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12162.1"
                     /translation="ELVGFSELKALCSKFPSTAFVCIHRLADDRMWSDALAMGAVDCC
                     HASNLQGILLASERYVVLTRSRSAAA"
     gene            complement(228..488)
                     /locus_tag="VFC15_18280"
     CDS             complement(228..488)
                     /locus_tag="VFC15_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM12163.1"
                     /translation="MKLRIDKSGRILLPKPLRKRLGLASETELEAVEQPDGVLLRVVV
                     QRASMIQVGGLWIHQGTAERGANWQRMLDDLREERIQAVLNL"
     gene            518..1444
                     /locus_tag="VFC15_18285"
     CDS             518..1444
                     /locus_tag="VFC15_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12164.1"
                     /translation="MAAQFPDMVRACAFVRTAAKIKTLLLLTKLICVFLKPIAQGVAV
                     QLRTGGYLVTKTEINAELQAIKKATDEFFSQVRVFMKGLYPDEVARGLESLINPYDLA
                     RKLLPELGNLGNDLRLQIGTLGAQYVNAVKQSPLLMEADGSDIRIAFRKMAAAVVLRS
                     YSYHESHVIAEEDRVFGMAPAEQSESQILPTEARSQFLCAGEELLEKLEFLLPAEQNL
                     TKAIVAAETPEIRSYRPNTAFIMMQISTDLPKLEDIRNSLKEVFKKFGIKAIRSDEIE
                     HQDVITQRILRRDCYKRISSGRPYGCSPECLL"
     gene            complement(1648..2901)
                     /locus_tag="VFC15_18290"
     CDS             complement(1648..2901)
                     /locus_tag="VFC15_18290"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZM12165.1"
                     /translation="MLTYEEIVALEQKYLLNTYARYPLAIARGKGVYVYDVKGRRYLD
                     FLTGLGVNALGHAHPRIVKDIREQAAQVIHVSNLYYNEYQGLLAEKLCTKSGLDRAFF
                     SNSGTEAIEGALKLVRAAGHATGGPEKCIVIALENSFHGRTMGALALTGQAKYRQGFD
                     PMLEGVRFVPRNDVEALRNAMDDKVCGIVLEPIQGEGGIQESSSEFLQACRQMADQNQ
                     AALIFDEIQCGLGRTGQLFAFQHFGVVPDVVCIAKPIAAGLPLGAFLANERFAQHITP
                     GKHGTTFGGGPLTCRVALEYLAILEDENLLEQVRRVGGYFSQKLRELVEKFDIAVESR
                     GMGAIQALQLTVPGKPILEGALAKGLLINVTQDTVLRFLPPFLLQEKHVDSGMRILRR
                     LLADAQKQEKAAARAAKSAAPGITA"
     gene            complement(3232..4077)
                     /locus_tag="VFC15_18295"
     CDS             complement(3232..4077)
                     /locus_tag="VFC15_18295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM12166.1"
                     /translation="MDSGTLTGAGLSLSAAAVWGGGDFAGGIATRRASVFRVVAGAHA
                     CGLLLMLLLAWITAEPFPPRSTLLWGAVAGIAGAFGIAALYQALSIGRMGIVAPVASV
                     ITGVLPVVAGMYSEGMPDRIQLAGFVLALVSIWLVARPDGEIDTHRGLGLAVLAGVMF
                     GLFLIAGKQAGHHGVFWPLVAARTVSTLLMLLIVVFSAADKRPLRPALVPILFSGILD
                     TSANALFIAATQHGRLDVTAVLSSLYPASTVVLARIFLKERISAAQNAGIVGALISVA
                     LIASR"
     gene            complement(4095..>4328)
                     /locus_tag="VFC15_18300"
     CDS             complement(4095..>4328)
                     /locus_tag="VFC15_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12167.1"
                     /translation="ASDYDRYFTAYPELRQHSTQPLTVETKIMPGAEQKGTVLVSFPV
                     TEQEFNARKDLNVTIVPYDQNSIVLRERSAAAK"
BASE COUNT          974 a         1270 c         1218 g          866 t
ORIGIN      
        1 gaactggttg gcttctccga actgaaggcc ctctgctcga aatttccttc caccgctttc
       61 gtatgcatcc atcgtctagc ggacgatcgc atgtggtccg atgctttggc tatgggcgcg
      121 gtggattgct gtcacgccag caacctgcaa ggcatcctgc tggcctcgga acgctacgtg
      181 gtgctgacgc ggtcgcggtc cgcggcggcg taaaaggcac agcgtcgtta gaggttaaga
      241 acggcttgga ttcgctcttc gcgaaggtca tccagcatgc gctgccaatt cgcgccgcgt
      301 tcggccgttc cttgatggat ccaaagtccg cccacctgga tcatcgatgc tcgttgcaca
      361 actacgcgca gcagaacgcc gtcaggctgt tccaccgcct caagttcggt ctcggacgca
      421 agccctagcc gttttcgcag cggtttggga agtaagatgc ggcctgactt gtcaatccga
      481 agtttcatgt tacagattat gccacttggg atgatccatg gcggcgcagt ttcccgatat
      541 ggttagagct tgcgctttcg tacgaaccgc tgcgaagata aaaacattac ttctattgac
      601 gaagctgatt tgcgtttttc tgaagcccat tgcgcagggc gtagctgttc agctcagaac
      661 aggtggatac ttagtgacaa aaacggagat caatgccgaa ctgcaggcaa taaagaaagc
      721 gacggacgaa tttttcagcc aggtgcgggt tttcatgaaa ggcctttacc ctgatgaggt
      781 cgcccgcggt ttggagagcc ttataaatcc ttatgattta gcgcgaaagc tcttgccgga
      841 actgggaaac cttgggaatg atctccgttt acaaattggc accctcgggg cccaatacgt
      901 caatgctgta aagcagtccc cgctcctgat ggaggccgat ggttccgaca tccgcatcgc
      961 cttcaggaag atggcagccg ccgtcgtact tcggagctat agttaccacg aatcacatgt
     1021 aatcgcggag gaagaccgcg tcttcgggat ggctcccgcg gaacaaagtg aatcgcagat
     1081 attaccaacc gaagccaggt cgcaattctt gtgtgcggga gaagagctcc ttgaaaaatt
     1141 ggaatttctc ctgccagcag aacaaaacct caccaaggcc atcgtggccg cagaaacacc
     1201 cgaaatcaga agctatcgcc cgaataccgc cttcataatg atgcaaatca gcacggatct
     1261 tcccaagctt gaggacatta ggaactccct gaaggaggtg ttcaagaaat ttggaatcaa
     1321 ggcaattcgg tccgacgaga tagagcacca agatgtcata acacagcgca tccttagacg
     1381 agattgctac aagcgaattt ctagtggccg accttacggg tgctcgcccg agtgtctact
     1441 atgaagtggg atatgcccac gcactaaaac gtcgaccgat tctatatcgt gagaaaggga
     1501 caccgctcca cttcgattta atggtgcata acgttcccga gtacgagaac attactgatc
     1561 tgaaaaagaa actgaccgca aggctcgccg cgctaaccgg gcgcgagcca tcggaatagg
     1621 cacttggtat gggtagcttg cgaatcgtta cgccgtaata cccggagcag cgctcttcgc
     1681 cgctctcgcc gcagctttct cctgcttttg cgcgtccgcg agcagcctgc gcaaaattcg
     1741 cattccgctg tctacgtgct tttcttgcag gaggaatggc ggcaaaaagc gcaacaccgt
     1801 gtcctgggta acgttaatca gcagaccttt ggcgagtgcc ccttccagga tcggcttgcc
     1861 ggggacagtc aactgcagcg cctgaatcgc gcccatcccg cggctttcca ccgcgatgtc
     1921 gaacttctcc accagttcgc gcagcttctg tgagaagtat cctccgacgc gacgcacctg
     1981 ctccagcaga ttttcgtcct ccaggatcgc gagatactcc aaggcgacac gacaggtcag
     2041 cggccctccg ccgaacgtcg ttccgtgctt gcccggagtg atgtgctgcg cgaaccgctc
     2101 gttcgcgaga aacgctccca ggggcagtcc ggcggcgatg ggcttggcga tgcacaccac
     2161 gtcaggcacg acgccgaagt gctggaaggc gaagagctgc ccagtacgtc caagtccgca
     2221 ttgaatttcg tcgaagatga gcgcagcctg gttctggtcg gccatctggc ggcacgcctg
     2281 cagaaactcg gacgacgatt cctgaatgcc accctcgccc tggatgggct caagcacaat
     2341 accgcaaacc ttgtcgtcca tcgcattgcg caacgcttcc acatcatttc gcggtacgaa
     2401 gcgcacgccc tccagcatgg gatcgaagcc ctggcgatac ttcgcctggc cggttagagc
     2461 gagcgcaccc atggtccgtc cgtgaaacga gttctccagc gcgatgacaa tgcacttttc
     2521 cggaccgccc gtggcgtggc cggcagcgcg caccagcttc aatgcgcctt cgattgcttc
     2581 tgtaccgctg ttggagaaga aggcgcgatc taatcccgac ttcgtgcata gcttctcggc
     2641 cagcagcccc tggtactcgt tgtagtagag gtttgagacg tggatgacct gcgccgcctg
     2701 ctcgcggatg tccttgacga tgcggggatg ggcatggccg agcgcgttaa ctcccagtcc
     2761 ggtgaggaag tcgagatagc gcctgccctt aacgtcgtaa acgtacactc ccttcccccg
     2821 cgcaatggca agcggatagc gagcgtaggt gttcagcagg tatttctgtt caagcgcaac
     2881 aatttcttca tacgtcagca cgatgatgac ctcgcaaaga gttcattgta acgcggggcg
     2941 aaggtggccc cgcctgggac agaggtgcga ggatttcgcg tggaaatcca gcaccgtaaa
     3001 cgcggctcag tttgggtaaa gtgggaaata gatccgagtc ccgtagccgg tgccccccaa
     3061 cgagcgccgt ttttgcgctt gttggggtgg ttgagggacg acacagttct cacacaccct
     3121 ctaaagcccc ggagtattta cgacaactta cgcgccggcc taaaggccga ctctacccga
     3181 acagcgactt gttcagagtt tccccagtac aacgccgaag cgtcgaggcc gttatcgcga
     3241 cgcgatcagg gccacagaaa taagcgcgcc cacaatgcct gcgttctgcg cagcggagat
     3301 gcgctccttc aggaatatcc gcgcaaggac cactgtgctg gcaggataaa gcgacgacaa
     3361 caccgccgta acgtcgaggc gcccgtgttg cgtggccgcg atgaaaagcg cattcgcgga
     3421 ggtatcgagg atgcccgaaa acagaatcgg aacgagagcc gggcgtaggg gccgcttgtc
     3481 ggctgctgaa aaaaccacga tgagcagcat gagcagtgtg gaaacagtgc gggccgcgac
     3541 cagcggccag aagactccgt gatggccagc ttgttttcct gcgatgagga acagtccgaa
     3601 catcacaccg gccaacacag ccagccccag accacgatgc gtatcgattt caccgtccgg
     3661 ccgtgcaacc aaccatatgc tgaccaatgc cagcacgaag cccgcaagct gaatgcggtc
     3721 gggcattcct tcgctgtaca tgccggccac cacaggcaac actcccgtga tgacggaagc
     3781 gaccggcgcg acgatgccca tgcgtccgat cgaaagcgcc tggtatagcg ccgcgatgcc
     3841 gaaagctcca gcaataccgg cgactgcgcc ccacaataac gtcgaacgcg gcgggaacgg
     3901 ctctgccgta atccatgcca acaagagcat gagcagcaag ccgcaggcgt gcgcgcccgc
     3961 gacaacccgg aagacgctgg cccgcctggt ggcgatgcct ccggcgaaat cgcctccgcc
     4021 ccagacggca gcagcgctca ggctgagacc ggcgccggtt aaagttccag agtccattaa
     4081 atttgaggag gacgctactt cgccgcagcg cttctctcgc gcagcacaat cgagttctga
     4141 tcgtagggca cgattgtcac attgagatcc ttgcgagcgt tgaactcctg ctcggtgacc
     4201 ggaaacgaca ccaggacagt acccttttgc tcggcgcccg gcatgatctt ggtctccacc
     4261 gtcaagggct gcgtcgaatg ttggcgcaac tccgggtagg ctgtgaagta tcgatcataa
     4321 tcggaagc
//
LOCUS       DASRKK010000762         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_276856, whole genome shotgun sequence.
ACCESSION   DASRKK010000762 DASRKK010000000
VERSION     DASRKK010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276856"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..636)
                     /locus_tag="VFC15_18305"
     CDS             complement(61..636)
                     /locus_tag="VFC15_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM12168.1"
                     /translation="MMRSFLFTLLLIAAVAATATAGTIEGKVSSGKSVVYVDTIQGKT
                     FPAPSQQPVMDQKGLMFNPHIMVVQVGTTVEFLNSDSVQHNVFWPSISGNKKLTHNLG
                     TWPKGEKKAFKFDNPGVVSLLCNVHPEMAGYIIVSPTPYFAETDASGNYKIENVPDGK
                     YNVVAWHEGMKQQSKSVDVAGTGKADFTLSK"
     gene            complement(678..2390)
                     /locus_tag="VFC15_18310"
     CDS             complement(678..2390)
                     /locus_tag="VFC15_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12169.1"
                     /translation="MWRNEEIDSHRGLQLIEEDNMQRHTVRTVRGSLLLLALAVTVLS
                     ASKSFAIPAFSRMYGTSCSTCHIDFPKLNDFGKAFKDAGFKFPKDDESMIKIPPVMLG
                     APANAELWPKAIWPGVIPGIPPIGLRYNNYFQYTGTSSSKFNQLLPPGTVPPFIPTTD
                     FQTGFFSIFTAGNFGSDIAFWVDDDISVSGANANGGLGEAYLKFVNIGRLMKLPSYAL
                     SLRVGQVELDIPFTQSKSIWISPYDIYTQANVGAVNAAFPLQQFVNNSFTFAGSGKGI
                     ELSGGHSTGGYHYSIAFIDQNTSGASQGSNNNYVPTATGSNFGGVGVASDSNFKDIYT
                     NFQYRFNLERDKESRNAIQAAGPTGPRDHTYLNVGSYWLYGRSVQRLSGATPDGLPTV
                     ITAHEPFYRVGGNMTFNYRCCLQFNALYMYGHDYNLLPIDANGFLVSLPVSGTPPPVG
                     FIHSRPATFSGGFADVEWLAYPWMMVLLRYDGVNSTSDYVNGLLPGGFTGSPFNGAAS
                     TTRNRITPGVQFLIHANIKASFEYQFRPSQSVVIGTNPITGFPVAMNPFHTNTAVAGL
                     EFVY"
     gene            2905..3105
                     /locus_tag="VFC15_18315"
                     /pseudo
     CDS             2905..3105
                     /locus_tag="VFC15_18315"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
     gene            3182..3355
                     /locus_tag="VFC15_18320"
     CDS             3182..3355
                     /locus_tag="VFC15_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12170.1"
                     /translation="MSFGLYSIGFVLVIAGLIYGAHLMHIPAHWIAVGAIVLLGLGVL
                     TGVKATRQKDPSS"
BASE COUNT          849 a         1001 c         1106 g          856 t
ORIGIN      
        1 acgaggtgca cgctgctgca agtggaagaa gggcggggcc ctgcccgccc ttggtaccgt
       61 ttatttcgag agggtgaaat cggcctttcc tgtgccggcg acgtcaacag acttgctctg
      121 ctgtttcatg ccttcatgcc aggcgaccac gttgtatttg ccatcgggga cgttctcgat
      181 tttgtagttc ccgctggcgt ccgtctcggc gaagtatggc gtcgggctga caatgatgta
      241 gcccgccatc tccgggtgaa cgttgcacag cagcgacacc acgccggggt tgtcgaactt
      301 aaatgccttc ttctcgccct tgggccaggt gccgaggttg tgggtaagct tcttattgcc
      361 gctgatggag ggccagaaaa cattatgttg cacactgtcg ctattcaaaa actcgacggt
      421 cgtccccacc tgtacgacca tgatatgcgg attaaacatc agacccttct ggtccataac
      481 cggctgctgc gagggggcgg gaaacgtttt gccctggatg gtatccacgt agacgacgga
      541 cttcccggac gacactttcc cttcgattgt gccagcggta gcggtggctg ccacggcggc
      601 gatcagcaaa agcgtaaata gaaaactgcg catcatgaat attcctttcg ttggttgtgc
      661 ttgctcagtt caagctgtta atacacgaat tctaaaccag cgactgccgt gttggtgtgg
      721 aacgggttca tagccaccgg gaaaccggtg atgggattag tcccgattac gaccgactgc
      781 gagggacgga actgatactc gaacgacgct ttgatgttgg cgtggatcag gaactgcacc
      841 cccggggtaa tgcggttccg cgtcgtgctg gccgccccgt tgaaggggga cccagtgaag
      901 cccccaggca acaggccatt cacatagtca gaggtggagt tcacgccgtc gtagcgtaac
      961 agtaccatca tccaggggta agcgagccat tcgacgtcgg cgaagccccc actgaacgtg
     1021 gccggccggc tgtgaatgaa cccgactggc ggcggcgtgc cgcttacggg caaagaaacc
     1081 aggaagccat tggcgtcaat cggcagcaag ttgtaatcgt gcccgtacat gtacagggca
     1141 ttgaactgca ggcagcagcg gtagttgaag gtcatgttgc ccccgacacg gtagaacggc
     1201 tcgtgcgcgg tgatgaccgt cggcaagccg tcgggggtcg cgcccgatag ccgctgtacc
     1261 gagcgtccat acagccagta gcttcccaca ttcaaatagg tatggtcgcg tggacccgtg
     1321 gggccggccg cttgaatggc gttgcggctc tccttgtcgc gctccaaatt gaagcggtat
     1381 tgaaaattgg tgtagatgtc tttgaagttc gaatccgagg cgacgccgac ccctccgaag
     1441 ttgcttccgg tcgcagtggg cacgtagttg ttgttgctgc cctgggatgc gccgctggtg
     1501 ttctggtcga taaacgcaat ggaatagtga tatccgccgg tcgagtgccc gccacttaac
     1561 tcgatgcctt tgcccgatcc ggcaaaggtg aatgaattgt tgacgaactg ctgcagcgga
     1621 aatgccgcat tcaccgctcc cacattggcc tgggtgtaga tgtcgtaagg gctgatccag
     1681 atggacttgg actgcgtgaa tggtatgtcg agctcaactt ggccgacgcg taggcttaac
     1741 gcgtaggagg gcaacttcat cagccggcca atattgacga acttcagata ggcctcgccc
     1801 aacccaccat tggcattggc tccgctgacg ctgatgtcgt cgtcaaccca gaaggcgata
     1861 tcgctgccga agtttccagc cgtgaagata ctgaagaagc ccgtctggaa gtcagtggta
     1921 ggaataaacg gcggtaccgt tccgggcggc agcaactgat tgaactttga gctgctggtt
     1981 cccgtgtatt gaaagtagtt gttgtaacgc aggccgatgg ggggaatgcc cgggatgacg
     2041 cccggccaga tggccttcgg ccagagttct gcgttggccg gtgctcccag catcaccggc
     2101 ggaattttga tcatcgactc gtcgtctttg ggaaacttga agcctgcatc cttgaaggct
     2161 tttccgaagt cattcagctt gggaaagtca atatggcagg tgctgcaact ggtgccatac
     2221 atacgtgaaa atgcgggaat tgcaaagctc ttgctcgctg acagaaccgt cactgcaagg
     2281 gccagcagta gcagggaccc acgaaccgta cggacggtat gccgttgcat gttgtcctcc
     2341 tcgatcagtt gcaagccacg atgagaatcg atctcttcgt tgcgccacat cggagccgct
     2401 gacaggaaaa gagagtattc atttcggcgc aacctcttag cagtgacgca cgtcacatga
     2461 cgctgtgatt cgtgcggcat tcgtggttgc gattcttgcc tccgtgacac tcttgtcgcg
     2521 actatgtacg ccagaatttg ggaagtaaat caaatcgtca gtgactcgga actaatcttg
     2581 aattccgctt ctgcgctttc gcatcgggcc cccttgtccg agggtttcca caagaatctc
     2641 gcgctttcgc tttttattcg gacggccaag gaatccgcta ccgaaccggt gactggctca
     2701 ggagtgggag ccggcgttca ggctacgacc caagaaaccc aagttcggct tcctgccgag
     2761 tccttgctta gactaggcgc catagcactg atttccaaga cggtcaattc tgaccagctt
     2821 tccgaatcct aaaggttgat actgccagtt cacgaggttc tgacggaaga aggaacttcg
     2881 tgacagccga catcgaagaa caaagcagcg ttgaaagcac acaagaattt ggacgaccag
     2941 agcatctcgt acagcgccaa gaatgggacg ctcgctctga agggtagcgc aaaaacccga
     3001 gcgcagcgaa acgaagcggc caaactcgcg caggctgttc ccaacgtcaa ggaagttgta
     3061 aatgagattg aggtgaagcc cgataagcgc tccacctcgc gataagtgca tcatgcagtg
     3121 aaaggcgggg cgcgctttgg gttcgcgcgg ctcgcgacta tttgatcgac aaggagatgc
     3181 aatgtcattt ggactctact ccattgggtt cgtgctcgtg attgctgggc tgatttacgg
     3241 cgcccacttg atgcacattc ctgcacactg gattgccgtg ggtgcgatcg tactattggg
     3301 cctgggggtc ctgacgggcg taaaagcaac ccgccaaaaa gacccgtcga gttagcggta
     3361 gttttccccg gctcctcttt tccgaatcgc attgtggtga ttcttcgatt cgaaaaaggg
     3421 ggcatatctt ggagatccat ggctccttaa gtgtcaaagg tcatgcttcc ttaaggagga
     3481 atgaaacaag gatgaatgag ttctgttggc taagattctc gccggccccg gtatttcgtg
     3541 gggggttccg cgcaggatac aaccagccaa tcaaagtcta aagacggaag aacgagcaca
     3601 gcttgtttgc cgaaaggcga taacgccgat gagttcaggc tcgctggaaa cctgtgcgag
     3661 ttggacttcg acgacgttcg agcgaacttc attcagcacg ggcgcatcac atttctcaag
     3721 cgaccgccct tagaagcggt ttttgcaata cccaaaggca ggtaatagct ctatgaacat
     3781 tcgagtaaga aacactctga gttggacgac tg
//
LOCUS       DASRKK010000763         2885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_768015, whole genome shotgun sequence.
ACCESSION   DASRKK010000763 DASRKK010000000
VERSION     DASRKK010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768015"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFC15_18325"
     CDS             complement(<1..1055)
                     /locus_tag="VFC15_18325"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZM12171.1"
                     /translation="MTTRMKDIAKDLGVSVMTISKVLRNHPDVGDDTRERVLARVREL
                     DYQPNLAARSLVTGRSYLVGLVVPDLLHPFFAEVAKSLSDVLRGSGFSLIISSSEEDP
                     AIEEQEINQLLARRLDALIIASCRSTVDLFFRIERRKTPYVLIDRTLPGLTANFVGVD
                     DEAVGRLATKHLIEIGCKRIAHIRGPETSTGVRRMDGFKQALTHAGLAIVDAYIISEP
                     KGDVETKQRGAEAMRQLLSLKPRPDGLFCFNDPLAMGAMNYALDQGVRIPEDLAVIGC
                     GNLHYDDSLRVPLSSIDQHSRQIGEQAARLALAILHSKVPLKPETVVLQPQLVVRGST
                     RRRAAKRVTARTVKRDIS"
     gene            1297..2340
                     /locus_tag="VFC15_18330"
     CDS             1297..2340
                     /locus_tag="VFC15_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM12172.1"
                     /translation="MKSRPASQRLIHGSVDPALVPQRVLCTGAKMPAIGLGTFGSDHV
                     TAAEIAAAVEAAAEVGCRHFDCASVYGNEPEIGDALQQVLRRQVKREELWITSKLWND
                     KHGEDDVIPSCRRSLADLRVGYLDLYLTHWPFPNYHPSGCDVSSRSKDAKPYILAEFM
                     KTWRKMEELVDLGLARHIGTSNMTIPKLKLLLRDARIKPAVNEMELHPHFQQPELFDF
                     LRTNRIVPVGYCPIGSPARPVRDRTPEDTVPTQDPVIVAIANRLGVHPAVVCVKWAVQ
                     RGQVPIPFSTDRNHILSNLEGAVSDPLTDGEMLAISTIDRNCRLIKGHVFLWKDGQSW
                     QDLWDMNGEITPP"
     gene            2377..>2885
                     /locus_tag="VFC15_18335"
     CDS             2377..>2885
                     /locus_tag="VFC15_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZM12173.1"
                     /translation="MMKNIEQSMRAAFLPGNSSVELRTVPVPRPSHGEALLRVKASTV
                     CGSDIRCIYHEHLGKGPEGYQGVIAGHEPSGQIVETGPGCRRFGVGDRVVVYHISGCG
                     VCNDCRRGYMISCTNEKYRRAYGWQRDGGMADYMIAEEKDLIALPQELTYADGAQVAC
                     GFGTVYEAI"
BASE COUNT          640 a          823 c          807 g          615 t
ORIGIN      
        1 gagatgtccc gcttcaccgt tcgcgccgtc actcttttcg cggcacgccg gcgcgtagat
       61 ccgcgcacga ccagttgcgg ttgcaacacg actgtctcag gtttgagcgg tactttcgag
      121 tgcagaattg cgagcgcgag acgtgcagct tgctccccaa tctgccggct gtgctgatcg
      181 atactcgaaa gtggaacccg caaagaatcg tcatagtgaa ggtttccgca accgatcact
      241 gcaaggtcct ctggaatgcg tacgccctga tccaatgcat agttcatagc gcccattgcc
      301 agcggatcgt taaaacagaa caaaccgtcg ggccggggct tgagactcag aagttggcgc
      361 atggcttctg cgccccgctg tttcgtttca acatctcctt tgggctcgct gatgatataa
      421 gcatcgacga ttgccaatcc ggcgtgcgtc agtgcttgct taaatccatc catgcggcgc
      481 acgcccgtgc tggtttccgg cccccgaatg tgagcaatcc gcttacagcc gatctcgatg
      541 aggtgtttgg tggccagcct tccaacggct tcatcgtcca ccccgacgaa attcgccgta
      601 aggcccggca aggtacgatc gatcaagaca taaggagtct tgcgccgttc gattcggaag
      661 aagagatcta cggtcgagcg gcacgaggcg atgatcaacg cgtcgaggcg gcgcgccaac
      721 agttgattga tctcctgttc ctcaatcgcc ggatcttctt ccgaggaaga gatgatcagt
      781 gagaatccgc tccctcgcaa aacatccgaa agagacttgg cgacttcggc gaaaaaggga
      841 tggagcaggt ccggaacaac caggccgacc agatacgacc gaccggtgac gagactgcgt
      901 gccgccagat taggctgata gtcaagctcc cttacgcgcg ccagaactcg ctcgcgagtg
      961 tcgtcgccca cgtcaggatg attccgaaga accttggaaa tggtcatgac cgagacccca
     1021 aggtctttcg caatgtcctt cattcgtgtg gtcatgactt gtcagccagt atacaaaacg
     1081 cgcatttccg caattttcat atccgatcct cgaccatcag acacgatgcc gccctgaaga
     1141 aaccgcagag gacgtaagaa tctggctgcg ctcaggagtt gttgacatcg gtaactttaa
     1201 cgattatcgt acatcgtgca caagctccgc tttgcacctt cgtatcggct tagtctccat
     1261 tcattggtct gatcaacgtg cgatgaggaa ttcaggatga agagtcggcc cgcgtcccaa
     1321 cgcctgattc acggcagtgt tgatcctgca cttgtgccgc agagagttct atgcactggg
     1381 gccaagatgc cggcaattgg cctggggacc ttcggttcgg accacgtaac ggctgccgaa
     1441 attgcggcag cggtcgaagc tgcggcggag gttgggtgtc ggcatttcga ttgtgcgtcc
     1501 gtttatggca atgaacccga aattggcgac gcgctgcagc aggtcctgcg ccggcaggtc
     1561 aagcgtgaag agctgtggat cacgtcgaag ctctggaacg acaagcacgg cgaagacgac
     1621 gtaattccat catgcagacg gtctcttgca gatctgaggg taggctacct cgatctctat
     1681 ctcacgcact ggcccttccc caattaccac ccatccggtt gcgatgtaag ttcccgcagc
     1741 aaggacgcaa agccctacat ccttgcggag ttcatgaaga cctggcgcaa gatggaggaa
     1801 ttagtggacc tggggctcgc tcgtcacatt gggacctcca atatgacgat ccccaagctg
     1861 aagcttcttc tgcgcgatgc ccgtatcaag cccgctgtga atgagatgga actacaccct
     1921 catttccagc agccggagct cttcgatttt ctgcgaacca accgcatcgt ccccgtggga
     1981 tattgtccga tcggatcacc agcccggccg gtccgcgatc gcacacctga agataccgta
     2041 ccgacccagg atcccgtgat cgtcgcaatt gccaatcgcc tgggagtgca tccagcagtt
     2101 gtctgtgtga agtgggcggt tcagcgcggg caagtaccaa ttccgttttc aaccgaccgc
     2161 aaccacattc tcagcaatct cgaaggggcc gtgagcgatc cgttgaccga cggagaaatg
     2221 cttgccatca gcaccattga ccggaattgt cgactaatca aaggccacgt gtttctgtgg
     2281 aaagacggac agtcatggca agacctctgg gacatgaatg gcgaaattac gcccccatga
     2341 ttgctgaatg accctgccct cttggactcg tcattgatga tgaagaacat tgaacagagc
     2401 atgcgcgccg cgtttctgcc tggcaacagc agcgttgaac tccgcaccgt gccggtacca
     2461 cgtcccagcc atggcgaggc cctgcttagg gtaaaggcat ccacagtctg tggctcggat
     2521 atccgatgca tctatcacga gcacctcggc aaaggcccgg aaggatatca aggggttatt
     2581 gccggtcatg agcccagcgg tcagatcgta gagaccggcc ccggctgccg gcgtttcgga
     2641 gttggcgacc gagtcgtcgt ctatcacatc tccggttgtg gggtctgcaa cgattgccgg
     2701 cggggctaca tgatctcttg cacgaacgaa aaatatcggc gtgcctatgg ttggcagcgc
     2761 gacggcggaa tggccgatta catgattgcc gaggagaaag acctcattgc acttccccag
     2821 gagctgacct acgcagacgg cgcgcaggtt gcgtgcggat ttggaaccgt ctatgaggcg
     2881 attga
//
LOCUS       DASRKK010000764         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1255494, whole genome shotgun sequence.
ACCESSION   DASRKK010000764 DASRKK010000000
VERSION     DASRKK010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255494"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..915
                     /locus_tag="VFC15_18340"
     CDS             247..915
                     /locus_tag="VFC15_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZM12174.1"
                     /translation="MKTEQQHRDEIVRFGKMLHQTGLVAATDGNLSVRLENGNVLCTP
                     TLMSKGLMEADDLVIVDQRGDKVSGCRGVSSELAMHLLIYRLRPDVGAVVHAHPPTAT
                     GYAAAGMALDSALCSEIIVTLGSVPLANYETPGTQELAEALAPLVAEHDAILMANHGV
                     VTCGADLLTAYMNMETVEHFAKIALVTHLLGKQQPLSDQHVDKLREMRVKYRNHVTVG
                     KGKE"
     gene            complement(1293..>2460)
                     /locus_tag="VFC15_18345"
     CDS             complement(1293..>2460)
                     /locus_tag="VFC15_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM12175.1"
                     /translation="ISGPGAWQHQFSGFEYHHVALNVNPLDDLDRPFDSAFNSLAKYN
                     LAGFAYQEIYSPRPWAQTTLGYNFQDENGYINNNSDFGISTTHGLRFNNYMFAQQTIV
                     WRRLSVLAGVGYVNNSSFGGKVSPRASLTFLALRGNTLFSGTRLHFGYSEGIKEPSFE
                     QTFGITGTFPTLPNPNLQPEQNRAIETGVLQSLFNNRVSLSAVYFHNQFRDQIEYVYN
                     SDTNTSQYVNFNRAMAQGAEVVLSGRVANHLSATAAYTYTSTQIQQAPPCDPNAGCDP
                     RIYGTGAPLLRRPKQVGMMLLTYTRQRWGGFLGGTAVGQRPDSDFLFGYIPPIYYAAG
                     YARLDLGGWYAIGHHVTAYANVNNALNNHYNEVLGYPALRASFRAGMRFSFGGE"
BASE COUNT          530 a          667 c          756 g          507 t
ORIGIN      
        1 caaagcgatt atgcctgagt gtctcctgcg gcccacctat aggaatgaaa attaaccaca
       61 gaggcacgga gatcacagag aagattcgaa agaagccaaa aactccgtgt cctccgtggt
      121 aaacgatttt cgggcgaacc gagcgtgcac tcgcccgcgc cagtttatga cttccctttt
      181 gcggaaaccg tttcctgcgg taggatagca atctaagcct ttccagcgca tccggggccc
      241 aaattcatga agaccgagca acaacacagg gatgagattg tccgcttcgg caagatgctt
      301 caccagaccg gtctggtggc ggcaaccgat ggcaatcttt ccgtgcgcct ggaaaacgga
      361 aatgtcctgt gcacaccgac cctgatgagc aagggtctaa tggaagcgga cgacctggtc
      421 atagtggacc agcgcggcga caaagtgagt ggctgccggg gcgtgtccag cgaactcgcc
      481 atgcacctgt tgatttaccg cctgcgtccg gatgttggcg cggtggtgca tgcgcatcct
      541 cccactgcga ccggctatgc ggctgccggt atggcgctgg acagtgcgtt gtgttcggag
      601 attatcgtga ccctgggttc ggtgccgctg gcgaactatg aaactcccgg cacgcaggag
      661 ctggcagaag cgctggctcc actggttgcc gagcacgatg ccatcctgat ggcgaaccac
      721 ggcgtggtca cgtgcggagc ggacttgctg accgcctaca tgaacatgga gaccgtggaa
      781 cattttgcca agatcgcatt ggtgacgcat ttgctgggta agcagcaacc gctcagcgat
      841 cagcacgtgg acaagctgcg ggaaatgcgg gtgaaatatc gcaaccacgt cacagtggga
      901 aaagggaaag agtaagttgt cctaaagaga tctgccagaa ctgtgccgtc cctgaaggga
      961 ctcgacttca ttttcaactt tacccagggc tcacgccctg ggctagtatt gttccgccct
     1021 tcgggctgga gttggcaaga tttaggcgat ctgctttctc accttgggtt cgcagcttga
     1081 gttctgaaga agacgtaaag agccgcgtga aggctaaaga tggcatttgc caagtggaat
     1141 ttgctggcga agaatccagc cccgtaggtg cggaactggt tttagcccag gacgtaagtc
     1201 ctgggtaaag cgagaaaaaa ctcacgagtc ccttcaggga cggcacaggt ctttcacacc
     1261 ccttttaaag tcccgactca cccaatcgtc gctcattctc ccccaaagct gaaccgcatc
     1321 ccggcgcgga agctggcccg cagagcggga tatcccaaca cctcgttgta gtgattgttc
     1381 agcgcgttat tcacattcgc gtatgccgtg acgtggtggc cgatggcgta ccagccgccg
     1441 agatcgaggc gggcataacc cgcggcatag tagatgggcg gaatgtatcc gaacaggaag
     1501 tcagagtccg ggcgctgccc gactgcggtg ccgccgagga atccgcccca gcgctgccgg
     1561 gtataagtca gcagcatcat gcccacttgc ttgggacggc gcagcagcgg agctcccgtg
     1621 ccgtaaatgc gcggatcgca acccgcattg ggatcgcaag gcggcgcttg ctgaatctgg
     1681 gttgaagtgt aggtgtaagc ggcggtcgcc gacagatggt tggcaacacg gccgctcaac
     1741 accacctcgg cgccctgcgc catcgcacga ttaaagttga cgtattgact ggtgttggta
     1801 tcggagttgt acacgtactc gatctggtcg cgaaactgat tgtggaaata cacggcgctg
     1861 agcgacacgc ggttgttgaa cagcgactgc agcacgccgg tttcgatggc ccggttctgc
     1921 tccggttgca ggttgggatt cggcagcgtg ggaaacgtac cggtgatgcc aaaggtttgc
     1981 tcgaagctgg gctccttgat tccctcggag tagccaaagt gcaggcgcgt accgctgaag
     2041 agggtgtttc cgcgtagcgc caggaacgtc agggaagcgc gcgggctgac tttgccgcca
     2101 aagctgctgt tgttgacgta gccaacgccc gcgagaaccg acagccgccg ccacacgatg
     2161 gtctgctgtg cgaacatata gttgttgaag cgcagaccgt gagtagtgct gattccaaag
     2221 tccgaattgt tgttgatgta tccgttctcg tcttgaaagt tgtaacccag cgtggtttgc
     2281 gcccacggcc gcgggctgta gatttcctga taagcgaaac cggcgaggtt gtacttggcc
     2341 aggctgttga aggcgctgtc gaatggccgg tcaagatcgt cgaggggatt cacgttaagc
     2401 gcgacgtggt ggtactcgaa gccgctgaac tggtgctgcc acgcgcccgg cccgctgatc
//
LOCUS       DASRKK010000765        14203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_669657, whole genome shotgun sequence.
ACCESSION   DASRKK010000765 DASRKK010000000
VERSION     DASRKK010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669657"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFC15_18350"
     CDS             <1..849
                     /locus_tag="VFC15_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12176.1"
                     /translation="IIFRLGGGIQGIAESKIYFVSTRGGSKEIWQMDYDGANQKQLTH
                     LGTIALSPHPSPDGSRIAYAGVSNAEWQILMYSLELNRPVNFTRFAGTNLSPAWSSDG
                     KTLAFSSSRSGDSEIYAVDISGGEVRRLTNTKGPDVSPTWNPKTNAQIAWVSGRTGLP
                     QIYTMASDGTNVVRMTDQGYAVSPSWSPNGQFLAFAWVRHYGPGAPGASDIYVMDVAS
                     KQWVQLTHDGGRNDFPTWSPDGRHIAFQSNRTGKTQIWSMLADGTQARQLTTAGENSQ
                     PNWSFK"
     gene            942..1691
                     /gene="pal"
                     /locus_tag="VFC15_18355"
     CDS             942..1691
                     /gene="pal"
                     /locus_tag="VFC15_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZM12177.1"
                     /translation="MKLRRIQWIQVVLALAVVMAAAGCAKKKPTPPTPVPPPPAPAPT
                     AMLTANPNTISAGQASTLTWTTDFASDVTIDGIGKVDPIGSMKVTPTESTTYRLVAKN
                     DTGTKEATARITVTPASAAPPPTSSSTGDESNARLFSQNVKDVFFDYDSYDMTSQDES
                     VIQADARFLQQHPNMNFVIEGHCDERGSIEYNLALGENRAQAAKQALVQAGVPATRMR
                     TISYGKEKPFCTASTEACWQQNRRAHFAYSK"
     gene            1922..2815
                     /locus_tag="VFC15_18360"
     CDS             1922..2815
                     /locus_tag="VFC15_18360"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM12178.1"
                     /translation="MRVRNHISGLAIVAILFASVYPAFGVSKEIIQLQTQVQTLSDQV
                     ARMQQSIDERLGVMRNLVEQSADSVNKMSVAVTDLQQKSQAQSADTNSKLEQVSGQIQ
                     SLHDTVDELKARLAKITKQLDDIQQGGQNLAAGQPPISAAPGAIAPSAGNQAAANSAP
                     PPDALYNNALRDYNAGKYDLSSQEFGDYLKYYGTTDLAGNAQFYLADIEYRQGNFDAA
                     VKDYDKVLEQYPGGSKAAAAQLKKGYALLELGQREAGVRELNSLIARYPRSIEASQAR
                     DRLKRLGVTTATPPPPSKKPH"
     gene            complement(2988..4370)
                     /locus_tag="VFC15_18365"
     CDS             complement(2988..4370)
                     /locus_tag="VFC15_18365"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12179.1"
                     /translation="MAEPDVPGSELKTIISELEKEKADFAVPASPRSRVATAVRALRH
                     RNFQLFFAGQLISLVGTWMQTVAQSWLVYRITGSTLLLGTVGFASQIPVFLVAPIGGI
                     VADRVNRHRVVVATQTSSMILAFILAFLTLTNRIHVWEIIVLASSLGVVNAFDIPARQ
                     AFLIDMVGREDLMNAIALNSSMFNGARILGPAIAGILVAWVGEGWCFFVNAVSYIAVI
                     VGLLLMRIVHPANLSHEGSPIENILEGFRFVRDAAPIRAILLMLGLVSLVGMPYSVLM
                     PVFAHQILHGGARELGILMGATGVGALLGALSLAAKVGLKGLGRVIAICAGGFGVSLI
                     LFSFSHVFWLSVILLVPVGFTMMVQMASSNTLVQSMSPDQLRGRVIAVYSMMFMGMAP
                     FGAFFAGAVAHHIGAPWTVALGGVACIAGAIAFGMHLPNIRGQARELILAQGMAAGSP
                     AEAMTSRGLS"
     gene            4569..6104
                     /locus_tag="VFC15_18370"
     CDS             4569..6104
                     /locus_tag="VFC15_18370"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZM12180.1"
                     /translation="MPTFAAVDIGSNSVRLKIAKSVRGRLHTLHEDREVTRLGESVFA
                     NGSLDPQAMSLTIKVLQRFHRSVQSFAADRVRVVATAALRDSRNSRAFLDWVRSATGW
                     KSEIITGLEEGRLIHLGVLSNARIANSRALLIDLGGGSCELTITVSGHIEDMVSLPIG
                     AVRLTQTFLHHDPPKKNELGQMRGFVQREISRIQKRILGAKVQTAIATSGTPAALSGL
                     YEAKVRGYDESKPHTVPHPAVSEILKELSKRNLDERRALPGIGPRRAEIIIPGAMVFA
                     ELMDMCALRNFRYLPLGLRDGLLAQMLADYGDSSAMKERVESERHDALLAVAEHYGTD
                     LAFSQRIRNLSMQLFRRLQSVHRLPPQYADWLEAAAMLHEVGAYINRSGRRRHTHYII
                     AQSELFGYTPAQRRIIAAIARYVGNSLPAASDAAMQMVPRSDQVEVPKAVALLRLARA
                     LDQGRRGAVRELHVRIHQEGPVRLGLAPATAEGVELELWAVEQERGYFKAAFGRDLFA
                     ELR"
     gene            complement(6056..6592)
                     /gene="sixA"
                     /locus_tag="VFC15_18375"
     CDS             complement(6056..6592)
                     /gene="sixA"
                     /locus_tag="VFC15_18375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZM12181.1"
                     /translation="MVIYFLRHANAGQKHFSDPVNDEKRPIDKLGEEQSHDVGRALAA
                     LGVTVNVIISSPLTRATQTAAIVSQELGHEEKLVLDDALRPEATFEKFKVLLNRHKDK
                     ASIMVVGHDPSMTEFVNRVLSGGGPLAVIEMKKGGVAKVEKDVRRQAVLKWCMPPKVV
                     QKIQRSSAKRSRPKAALK"
     gene            complement(6807..7418)
                     /gene="nuoB"
                     /locus_tag="VFC15_18380"
     CDS             complement(6807..7418)
                     /gene="nuoB"
                     /locus_tag="VFC15_18380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZM12182.1"
                     /translation="MGWINNRFEKNFLVTTVDYVFNWARKSAIWPMTFGLACCAIEMI
                     AASTSRFDIARFGSEVFRPSPRQSDLMIVSGTVTLKMAPVVKRIYDQMPEPKWVISMG
                     ACSSVGGPFNTYAVLQGVDRIVPVDVYIVGCPPRPENLFYGLLRLQDKIDQMTIAKKP
                     TEVRLREDMVDNFKEQIRVAQANFANPEQLVMPVETPIVGGGR"
     gene            7611..8498
                     /locus_tag="VFC15_18385"
     CDS             7611..8498
                     /locus_tag="VFC15_18385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM12183.1"
                     /translation="MPKSLRRLSERNPFLSVLYNLPRLAKGYQPIYLDYPVNPVPRYG
                     YGKPLHPELSGILASGSADYVRLLTDFLKFESNLLAIPVRQPKTSEEPCWVNKWLGGL
                     DTFALYGFVAACNPAVYIEVGSGFSTKVVRRAIRDLSLSTRLVSIDPSPRAEIDRLCD
                     EVIRQPLEDCDLSVFDCLGPGDIFFMDGSHRSFMNSDVTVFFLEILPRFKPEVFVHIH
                     DIYLPFDYPPDRAYWYYSEQYLLAASLLAGHQGYDIVLPNNYISNTPEASSVLEPLWA
                     SPQLVGVSGNGSSFWLKTR"
     gene            8571..9917
                     /locus_tag="VFC15_18390"
     CDS             8571..9917
                     /locus_tag="VFC15_18390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM12184.1"
                     /translation="MSNDLKSSAGQPHHNLTAVDRAPKSILDSILHSPGWMVLIGFSL
                     RVLYIALAHSYKFRTTDSNFSFGWEIGRIAYSLANGHGFSSPFGGDTGPSAWTAPVYP
                     WIVSLAFRAFGSYSNAASFALLTFNSLCSALTAWAIYCTGRRVFNEKVALWSGWIWAL
                     LPYIMYWSVRWIWETSLTALLLSLAFMLTVEMEGDDRLTSWTGYGVLWGVIGLTNPST
                     LSFVPCAGCWLAYQLYRRGKRFVLPAIVAAVLFWMTMMPWLVRNYMVFHQPVFVRDNV
                     GVEIRCGNNPLAEGVWVGMYHPSQNPILYRQYQEMGELAYSVEQSRLAKQWIAGNPKQ
                     FANLTFRRVVFYWNGLPRLSKIQALEQFKNSLFLASSLLSIAGLVLAIKHRVHGVFLF
                     ASLLLVYPLIYYVTFPQPRYRHPIDPELVILGVYLFTQVSPRSPHLKGTSQPSHAD"
     gene            9907..10653
                     /locus_tag="VFC15_18395"
     CDS             9907..10653
                     /locus_tag="VFC15_18395"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM12185.1"
                     /translation="MPTEDPGVPQFRTLSIIIPVYNERQTVMQLLQQVAQQPLSLHKE
                     LIIVDDCSTDGTREFLRQADLEHVLGNNGTNSVKLALHEKNQGKGAGVRTGLTQATGE
                     IVLIQDADLEYDPSDYPILIAPILEGHADAVFGNRFHNGPHRVPRYYRYLLNRFFSML
                     CNSLTGIAIHDVTACYKVFRRELLERMHLRSDRFSIETEMTVKLAKMRVRIYEVPIVY
                     HGRTYAEGKKISWKDGFIALYDLIRYRFKD"
     gene            10747..11025
                     /locus_tag="VFC15_18400"
     CDS             10747..11025
                     /locus_tag="VFC15_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12186.1"
                     /translation="MHHSRKSVLIKAALGLSLMAAVFALSAEAQVTQDFHRTIPLSST
                     GRVSLENINGNVTVTGWERNEVQIDAVKKARDQQKLDEARIEVDATSD"
     gene            11080..11526
                     /locus_tag="VFC15_18405"
     CDS             11080..11526
                     /locus_tag="VFC15_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM12187.1"
                     /translation="MTYELHVPRLARLDHINLVNGSLEVSQVTGEIDATLVNGKTDLR
                     DLSGRLTISAVNGTITANYRSLDNVKEIHLKSVKGAINLGLPASPNAEVNASTVNGGI
                     TTDFPLQVKGKFMGHRLNGTLGNGGTRIELSNVNGSIRLGPGLGNL"
     gene            complement(11645..12394)
                     /locus_tag="VFC15_18410"
     CDS             complement(11645..12394)
                     /locus_tag="VFC15_18410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM12188.1"
                     /translation="MSDRRTGSADEQLRQEFNRWAEQGKGEEMEAHHISITEQTLALI
                     KLKAGDRVLDLGCGAGWASRLMAKAVDDAARPGQVIGVDVSDEMIRRARAASTDYDNV
                     MFVVGSAQQVPWEENFFDKVLSVESFYYYADQERALAEMFRVMAPKGELYILINLYRD
                     NHYSLRWVEELKVPVQVHSEQEYIEMLQRHTFEDVRAVRVSDLTPTPEEYSGRWFKNA
                     EELRDFKRIGALLLIARKPDFESPPPAYQLY"
     gene            12621..14054
                     /locus_tag="VFC15_18415"
     CDS             12621..14054
                     /locus_tag="VFC15_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM12189.1"
                     /translation="MPQSVHASTEHSTHHEPTLVRGLSLMDSVMLMVGGIIGSGIFLT
                     AGQIATAVHKPLYFLGVWFVGGLISLLACFAFAEMAAMYPQSGGQYVYLREAYGEFPA
                     FLYGWMIFTVGQSGTIAALAVGFAEYFGAIVPLAAAHKPIATFLGFTLTRGHLVSIGA
                     IVLLTAVNVVGVKRGSALINTATWAKFAAIGIFVLLGLAVGKGSWSNYAVNLPLDQPA
                     SLSHVISGFGVALIAVFWAYDGWVYVTWVSGEIKNPQRVLPRALLLGILLVGVIYVSV
                     NVIYLYALPITQVANETTIAQAAAVTLFSSNAAKWLGATIALSCFGAASSAILCSARV
                     SYAMARDGAFFHAMGRVHPKYRTPAFSLIALSAWSVVLALSGSYDQLYTYVMFMMVLS
                     YVAAVAALFVLRRKRPDVPRPYLCTGYPYLPALYLLMAGAWALNSVVTRPKETLVGIG
                     IVFLGAPFYAFWRWQLKRHPEELKEFE"
BASE COUNT         2943 a         4583 c         3755 g         2922 t
ORIGIN      
        1 atcatcttcc gtctcggtgg aggcatccaa ggtattgcgg agtccaagat ctatttcgtc
       61 agcacccgcg gcggctccaa agaaatctgg cagatggatt acgacggcgc caaccagaag
      121 caactcaccc atctcggcac cattgcccta tccccgcatc cgtcgccgga tggctcccgc
      181 attgcgtatg caggcgtatc gaacgcggag tggcaaatcc tgatgtactc gctggagttg
      241 aaccgaccgg tgaactttac gcgctttgcg ggcaccaatc tctcgcccgc atggtcgtcc
      301 gatggaaaga cactcgcgtt ctcgtcatcc cgctccggcg attccgaaat ctatgccgtg
      361 gacattagcg gcggcgaggt tcggcggctg accaacacga aaggccccga cgtctcgccc
      421 acctggaatc ccaagacaaa cgctcagatc gcgtgggtca gcggtcgcac gggtttgcca
      481 cagatctata ccatggcgtc ggacggaacc aacgtcgttc gcatgaccga ccagggctac
      541 gcggtctcgc cttcgtggtc gcccaacggg cagttcctgg ccttcgcatg ggtgcgccac
      601 tatggtccgg gcgcccctgg cgccagcgat atttacgtta tggacgtcgc cagtaaacag
      661 tgggtgcagc tcactcacga tggcggacgc aatgattttc ccacctggtc gcccgacggc
      721 cggcacattg cattccagtc gaaccgtacc ggcaagaccc agatatggag catgctggcc
      781 gatggcactc aggcgcggca attgacgacg gcgggcgaaa actcgcagcc gaattggagt
      841 ttcaaataga ttaggtcgca gataaagcga acgagcggaa ggagcacaat ccgccctgca
      901 ccgaaactga agcgagtgct ctcgcaatcg gaggtacgat tttgaaacta cgacggattc
      961 agtggatcca ggttgtcctt gcgctggctg tggtcatggc agcggctggc tgcgccaaga
     1021 agaaaccgac gccgcccaca ccagttccac cgccccctgc cccggcgccc acggctatgc
     1081 tgaccgcgaa tcccaacacc atctctgctg gccaagcctc gacattaacc tggacgaccg
     1141 acttcgccag cgacgtcacg atagacggta tcggcaaggt tgaccctatc ggctcgatga
     1201 aggtcacgcc caccgagtcc accacgtatc gcctggtggc gaagaatgac acgggcacca
     1261 aggaagccac ggctcgcatc accgtgactc ctgcttccgc ggctccgcct ccgaccagca
     1321 gttcgacagg cgacgaaagc aatgcgcgcc tgttctcgca gaacgtcaaa gacgtgttct
     1381 tcgactacga cagctacgac atgacgtcgc aggacgagtc cgtgatccag gcggacgcgc
     1441 gtttcctgca gcagcatccc aacatgaatt ttgttattga gggccactgc gacgagcgcg
     1501 ggtcaatcga atacaatctg gcgctgggcg agaatcgcgc ccaggcggcc aagcaggcac
     1561 tcgttcaggc aggtgtgccg gcaacccgca tgcgcaccat cagctacggc aaggagaaac
     1621 cgttctgtac cgcgagcacc gaagcctgct ggcagcagaa ccgccgcgcc cacttcgctt
     1681 actcgaagta acgggcggtt gtcgttggct taggagcggc gcctgcacga cctgcgccgc
     1741 ttcccttatg caacacacca tccgcgtagc ggaggctgcg tagcgcaagc cccgggtgca
     1801 gtggccagga atgacccgag ccgcgtagcg gcgaggcagg aaccaatact ccaccctgat
     1861 gaccttcgcg atttacgata gtcttttcaa gacggcaatc ttccaatcag gaaacctatc
     1921 tatgcgagtt cgaaaccaca tatccggtct ggccatcgtc gccatcctgt tcgcgagcgt
     1981 gtatcccgcc ttcggcgtca gcaaggaaat catccaactg caaacccagg tgcagacgct
     2041 ctcggatcag gtcgcgcgca tgcagcaatc catcgacgaa cgtctgggcg tcatgcgcaa
     2101 cctcgtcgag cagagtgccg acagcgtcaa caagatgagt gttgccgtca ccgacctgca
     2161 gcagaaatcg caggcacagt ccgctgatac gaacagcaaa ctggagcagg tatccggcca
     2221 gattcagtcg ctgcacgaca ccgtggacga gctaaaggcg cggcttgcca agatcactaa
     2281 acagctcgac gacatccagc agggtggcca gaacctcgcc gccggacagc ccccgatatc
     2341 agccgctccg ggcgcgattg ctccctcggc aggaaatcag gccgctgcga attcagctcc
     2401 tcccccggac gcgctctaca acaacgcgct gcgcgactac aacgccggca agtatgacct
     2461 ctcgtcgcag gaattcggag attacctgaa gtactacggc accaccgatc tcgcgggcaa
     2521 tgcgcagttc tatctcgccg acatcgagta tcgccaaggc aacttcgatg cggctgtgaa
     2581 agattacgac aaagtgctgg aacagtatcc cggtggaagc aaggctgcgg cagcacaact
     2641 gaagaagggc tatgcgctat tggaactcgg ccagcgcgaa gccggagtgc gcgaactgaa
     2701 cagcctcatc gcccgctatc cacgctccat cgaagcttcg caagcccgtg accggctcaa
     2761 gcgtctggga gtcacgacgg ccactccgcc cccgccgtca aaaaagccac actagggacg
     2821 atcccgtata tccacttgta ggggcacgcc ttcacgggct gctgacatgt cgaatgggtc
     2881 gaagagctgg cctttaggcc agcggttagg cgtttatttt tgatgtctcc cgcggacttc
     2941 agtccgcggg agacctgctt tttctcttgt gtaggtaacc aactgtctca cgacaatcct
     3001 cgactcgtca tcgcttccgc cggggaaccc gccgccatcc cctgcgccaa tatcaattca
     3061 cgcgcctgac cacggatgtt gggaaggtgc ataccgaacg caattgcgcc cgcgatgcac
     3121 gctactccgc cgagtgctac cgtccacggc gccccgatat gatgcgcaac ggctcctgca
     3181 aaaaacgccc caaacggagc cattcccatg aacatcatcg agtacacggc aatcactcgt
     3241 ccccgcaatt ggtcgggcga catcgattgc accagagtat tcgacgatgc catctgtacc
     3301 atcatggtga atcccaccgg caccagcaga atcactgaaa gccagaacac atgcgagaag
     3361 gaaaacagga taaggctgac gccaaacccg ccggcgcaga tcgcaatcac ccgtcccagt
     3421 cccttgaggc ccaccttcgc agccaggcta agcgcgccaa gcagtgcacc cacgccagta
     3481 gcgcccatca gaatgccgag ttccctggcg ccgccatgca gaatctgatg cgcgaagaca
     3541 ggcatcagta cggaatacgg cattcccacc aggcttacca atcccagcat cagcaagatc
     3601 gcgcggatcg gcgccgcatc ccgcacgaag cggaatccct ccagaatgtt ctcgatcggc
     3661 gagccctcat ggctcagatt cgccggatgc acaatccgca tcaacaacaa gccgacaatc
     3721 accgcaatgt agctcacggc gttgacgaag aagcaccaac cctcgccgac ccatgccacc
     3781 agaatgcccg cgatggctgg gccaagaatg cgcgcgccgt tgaacatcga cgagttcaac
     3841 gcaatcgcgt tcatcaggtc ttcgcggcct accatgtcga taagaaacgc ctgccgcgcc
     3901 ggaatatcga acgcgttcac gactccgaga ctagacgcca gcacgatgat ttcccagaca
     3961 tggatgcggt tggtcagtgt gagaaacgcc aggatgaacg ccaggatcat cgagctggtt
     4021 tgggtcgcga ccaccacacg gtggcgattc acgcgatcgg ccacgatgcc gccgatcgga
     4081 gcgaccagga acaccggaat ctggctggca aatccaactg ttcccagcaa cagtgtcgag
     4141 cccgtgatgc gatacaccag ccacgactgc gcgaccgtct gcatccatgt cccgaccagc
     4201 gagatcaact ggcctgcaaa gaacaactgg aagttgcgat gccgcagcgc gcgcaccgcc
     4261 gtggccaccc tcgaccgcgg cgacgccggc actgcaaaat ccgctttttc cttctccagc
     4321 tcagagataa tcgtcttcaa ctccgagcct ggaacatccg gttcagccat aattttattg
     4381 gatgcgccaa ctcaaacacc tgcttccaat ccggcaatcc gctctcacgt cgtcgccatc
     4441 ctcaagcata cggcaatgca ctcatgcttc gtgccacgcc cagtcggttg ccatccgcgt
     4501 gatacgcagc tatcgtgcta acggttgcgc ttgcgaagaa aatcggtgcg catccagcac
     4561 cctgacccat gcccacattt gccgcggttg atatcggttc caactctgtc cgcctcaaga
     4621 tcgccaaatc cgtccgcgga cgcctgcaca ccctccacga ggaccgcgaa gtaacacgcc
     4681 tgggcgaaag cgtgttcgcc aacggttctc tcgatcccca ggcgatgtcg ctcaccatca
     4741 aggtgctgca gcgctttcat cgctcggtgc agagcttcgc cgccgaccgc gtgcgggtgg
     4801 tcgctaccgc cgcgctgcgc gactcgcgca acagccgggc ctttctcgac tgggtacgtt
     4861 ctgccacggg atggaagtca gagatcatca ccggccttga agaaggacgt ctcattcatc
     4921 tcggcgtcct gtccaatgcc cgcatcgcaa acagtcgcgc cttgttgatt gacctcggcg
     4981 gcggcagttg cgaactgact atcacggtgt caggtcacat tgaggacatg gtcagcttgc
     5041 ccatcggggc ggtgcgcctg acccagactt tcctgcacca tgatccgcca aagaaaaacg
     5101 aactcggaca gatgcgcggt ttcgtgcagc gcgaaatttc gcgcattcag aagcgcatcc
     5161 tcggagcgaa agtacaaacc gccatcgcca catcgggcac acccgcggcg ttatcgggac
     5221 tttacgaagc caaggtccgc ggctatgacg aatccaagcc ccacaccgtg ccgcatcccg
     5281 cagtctcgga gatcctcaag gagctgtcca agcgcaacct cgatgagcgg cgcgccctcc
     5341 ccggcatcgg gccgcgacgc gccgaaatca tcatccccgg cgccatggtc tttgccgaat
     5401 tgatggacat gtgcgccctg cgcaacttcc gttatctccc acttggcttg cgcgacggat
     5461 tactggcgca gatgctggcc gactacggcg acagctcagc catgaaagag cgcgtcgagt
     5521 ccgaacgtca cgacgcgctc ctcgctgtag ccgagcacta tgggaccgat ctcgcatttt
     5581 cacagcggat tcgcaacctc tccatgcaac tcttccgccg cttgcagtcg gtgcatcgtc
     5641 tgccgccgca gtatgcggac tggctcgaag ccgcggccat gctgcacgaa gtgggcgcct
     5701 acatcaatcg ttccggacgc cgccgccaca cccactacat cattgcccag tccgaactct
     5761 tcggctacac cccggcacaa cgccgcatta tcgcagccat cgcgcgctac gtgggcaatt
     5821 cccttcctgc cgcgagcgac gctgccatgc agatggttcc ccgctcggac caggtcgaag
     5881 tccccaaggc cgtcgcattg ttgcgtctgg cgcgcgcgct cgatcaggga cgccgcgggg
     5941 cggtgcgcga gttacacgtc cgcattcatc aggagggacc cgtgcgcctc ggcctcgcgc
     6001 cggctaccgc agaaggcgtt gaactggagc tttgggcggt tgaacaagaa cggggttact
     6061 tcaaggccgc cttcggacgc gacctcttcg ccgaacttcg ctgaattttc tgcaccacct
     6121 tgggcggcat gcaccacttc agcacagcct gccggcggac gtctttctcc accttggcca
     6181 cgccgccttt tttcatctca atcaccgcca gcgggcctcc gccggagagc acccgattca
     6241 cgaactccgt catgctcgga tcgtgcccaa ccaccatgat ggacgccttg tctttgtggc
     6301 ggttcagcag caccttgaac ttctcaaacg tcgcctcggg ccgcaaagcg tcatcgagca
     6361 cgagcttttc ctcgtgaccc agttcctgcg acacgattgc cgcagtctgc gtcgcccgag
     6421 tgagcgggct agagataatg acattcacag tcacgccgag agccgccagc gcacgtccca
     6481 catcatggga ctgctcctcc ccaagcttgt cgatcggacg cttttcgtcg ttcaccgggt
     6541 cgctgaaatg cttctgccct gcgttcgcgt gcctgaggaa ataaatgacc attatcgtat
     6601 tgtgccctaa aagttgggct gaggctccat tcttgaaagg tgctgtatcc ggatgtgcac
     6661 cggtcggtgg caaggcaacc ggcaccccag ccaacagagc ccgacccttg gccgagtcgc
     6721 ctcgaagctt ctcgatcaca cgcagcgcga cccgtgacgc aaaaaagggc ccgctttgcg
     6781 agcctttgac ctgcaaaaaa aatgtgtcag cggccgccgc cgacgatcgg tgtctcgacc
     6841 ggcatcacca actgttcggg attggcaaaa ttcgcctgcg caaccctgat ctgctccttg
     6901 aagttatcga ccatatcttc ccgcagccgg acttccgtcg gcttcttggc gatggtcatc
     6961 tggtcgattt tgtcctgcag ccgaagcagt ccgtagaaaa ggttttctgg ccgcggcgga
     7021 caacccacga tatacacgtc cactggcacg attcgatcga caccttgcag caccgcatag
     7081 gtgttgaacg ggccacctac ggacgaacac gcacccatcg agatcaccca cttgggctcg
     7141 ggcatctggt catagatccg cttcaccacg ggagccatct tcagtgtcac cgtgccgctg
     7201 acgatcatca agtcgctctg tcgtgggctg ggacgaaaca cctccgagcc aaaccgcgca
     7261 atgtcgaaac gcgaggttga ggccgcgatc atctcgatcg cacagcacgc caggccaaac
     7321 gtcatcggcc agatggcgga tttccgcgcc cagttgaaaa cgtagtcgac cgtcgttact
     7381 agaaaattct tttcgaaccg attgttgatc caacccatga agccctctcc tgctcgcagg
     7441 ttcctgactc tccatcttcg ccgaacgctc tgcctgaggg ctgtgatcac ggtcacaggc
     7501 cacccgctaa agtgttctga gtctaagttc aattgtccgc agttacaagc ggcttgggag
     7561 gcgggaaagg cctttccgcc caagatcagc acagagtagg atgttcggtg atgcccaaaa
     7621 gtttacgcag gctgagcgaa cggaatccct ttctgtctgt cctttacaac ctccccagac
     7681 tcgcgaaggg ataccagcct atctacttag attatccagt caaccctgtt ccgcgctacg
     7741 gatatgggaa accgttacac cctgagctgt ccggcattct tgccagcggc agcgctgatt
     7801 acgtgcggct cttgaccgac ttcctgaaat ttgaatcgaa cctgttggca attcctgtcc
     7861 gtcagcccaa aacttccgaa gagccctgct gggtgaacaa gtggctgggt ggactggaca
     7921 cgttcgcgct gtatggattt gttgctgcgt gcaatccggc tgtgtatatc gaagtgggca
     7981 gcgggttctc aacgaaagta gtgcggcggg ctatccggga tctgtcactt tccaccagac
     8041 tagtttcgat tgacccctct cccagggctg agatcgatcg gttgtgcgac gaagtcatca
     8101 gacaaccact ggaagactgt gatctctctg ttttcgattg ccttggtccg ggcgacattt
     8161 tttttatgga cggatcgcac cgcagcttca tgaactcgga tgttacggtc ttcttcctgg
     8221 agattttgcc gcgcttcaag cccgaggtct ttgtacacat ccacgacatc tacttgccat
     8281 tcgactatcc gcctgatcga gcctattggt actattcgga gcagtatttg ttggccgcca
     8341 gcctgcttgc cggacaccag ggatacgaca tcgttcttcc caacaactac atcagcaata
     8401 cacccgaagc atccagtgtt ctggaaccac tgtgggcctc tccacaattg gtcggagtct
     8461 caggaaatgg ctcttctttc tggctgaaga cccgatgatt ttcatccagc ccggcaagac
     8521 cgccatcgat acgactattc tcaccgctct ctgcggtaaa atcggatttc gtgtccaatg
     8581 atctgaaaag ctcagcgggt cagccccacc ataacctcac tgcagtcgat cgcgcgccga
     8641 agtccattct cgatagcatc ctgcactccc ccggttggat ggtacttatt ggattctcac
     8701 tccgcgtgct ctacatcgcg ctcgcccact cctacaagtt ccgcaccacc gattccaact
     8761 tcagctttgg ctgggagatc ggacgcatcg cgtactcgct ggccaacggt cacgggttca
     8821 gttctccttt cggcggcgac accgggcctt ccgcgtggac cgcgccagtt tatccctgga
     8881 tcgtctcgtt ggccttccgc gccttcggta gttactcgaa tgccgcgtca ttcgcgctat
     8941 tgacctttaa cagcctctgc tctgcactta ctgcctgggc gatctactgc accgggcggc
     9001 gcgtgttcaa tgaaaaagtg gccttgtggt cgggatggat atgggcgctg ctgccctaca
     9061 tcatgtattg gtcggtgcgc tggatctggg aaaccagcct caccgcttta ctgctgagcc
     9121 tggctttcat gctgaccgtc gagatggaag gcgacgaccg cctcacgtcg tggaccggct
     9181 acggagtgct atggggcgtg atcggtctca cgaatccctc gaccctttcc ttcgtgccct
     9241 gcgccggatg ctggctcgcc taccaactct accggcgcgg taagcgattc gtgcttcccg
     9301 ccatcgtcgc cgcagtcctc ttctggatga ccatgatgcc ctggctggta cgcaactaca
     9361 tggtcttcca ccagccggtc ttcgtgcgcg ataacgtcgg agtcgagatc cgctgcggca
     9421 acaatccgtt ggctgaaggt gtttgggtgg gcatgtatca tcccagccag aacccgatcc
     9481 tctaccgcca atatcaggag atgggcgaac tcgcctattc cgttgaacaa tcgcgactcg
     9541 ccaagcagtg gatcgccggg aatccgaaac agttcgccaa cctgacgttc cgccgcgtcg
     9601 tcttttactg gaatggactg ccgcgccttt cgaagattca ggcgctggag caattcaaga
     9661 actcgctctt cctggcctcc tccctgttgt cgatcgctgg actcgtgctc gccatcaagc
     9721 atcgcgtcca cggcgtcttt ctgttcgcca gcctgctgct tgtttatccg ctgatttact
     9781 atgtaacttt cccgcaacct cgttaccggc atccgattga cccggagctg gtgattctgg
     9841 gagtttattt gttcacccaa gtcagtccgc gtagcccaca cttgaaaggc acgagccagc
     9901 ctagccatgc cgactgaaga tccgggcgtt ccgcagttcc gcacgctttc catcatcatt
     9961 ccggtctata acgaaagaca gaccgtgatg caactcctcc agcaggtggc ccagcaaccg
    10021 ctctcgctgc acaaggagtt gatcattgtg gacgactgct ccaccgacgg cacacgcgag
    10081 ttcctgcggc aggccgatct ggaacacgtg ctgggaaaca acggcacaaa ctccgtcaaa
    10141 ctcgcgctgc acgaaaagaa ccagggcaag ggcgcgggcg tccgcactgg actgacgcaa
    10201 gccaccggcg agatcgtcct gatccaggac gccgatctag agtacgaccc cagcgactat
    10261 cccatcctca tcgcacccat tctcgaaggc catgccgatg ccgtcttcgg caaccgtttt
    10321 cacaatggcc cacaccgcgt tccgcgttac tatcgctacc tcctcaaccg ctttttctcg
    10381 atgctgtgca attcgctcac cggcatcgcg attcatgatg tcaccgcctg ctacaaggtc
    10441 ttccgccgcg agttgctcga acgtatgcac ctgcgctccg accgtttcag catcgaaacg
    10501 gaaatgaccg tgaagttggc gaaaatgcgc gtacgcattt acgaggttcc catcgtttat
    10561 cacgggcgaa cgtatgcgga aggcaagaag atcagttgga aggacggctt tatcgctctc
    10621 tacgacctca ttcgctatcg ctttaaagat tagttgcagc cgccgcgtaa ccctcaaatg
    10681 aagctacctg aacaattcat ctaactcttc gtatctctcc tcgtctaact cgcgacagga
    10741 ggaaccatgc accattcccg caaatcggtt cttattaagg cagctctcgg gctttcgttg
    10801 atggcagctg tgttcgccct ttcggctgaa gcgcaggtca cacaggattt tcaccgtacc
    10861 attcctctct cctctaccgg ccgcgtctct ctcgaaaata tcaacggcaa tgtcaccgtc
    10921 accggatggg agcgcaatga agtccagatc gatgccgtca agaaggcgcg tgatcagcag
    10981 aagctcgacg aagcccgcat cgaggttgac gccaccagcg actgagtcca aatcaagacc
    11041 aagtaccccg aaggccacaa caacaacaat ccagcgaccg tgacttacga actgcatgtg
    11101 cctcgtcttg cgcgcctgga tcacatcaat ctggtgaacg gttcgctcga agtgtcgcag
    11161 gtaaccggcg agatcgatgc gactctggtg aatggcaaga ctgatcttcg tgacctttcc
    11221 ggacgtctga ccatctcggc agtcaacggc accatcaccg cgaattaccg ctcactcgac
    11281 aatgtgaagg agatccatct gaagtcggtg aagggagcca tcaatctcgg tttacccgct
    11341 tcgcccaacg ccgaagtcaa cgcgtcgact gtcaacggag gcattaccac cgacttcccc
    11401 ctgcaggtca aaggcaagtt catgggtcac cgtctcaatg gcacgctcgg caatggcggc
    11461 actcgtatcg aactcagcaa cgtaaatggc agcattcgac tagggcccgg actggggaat
    11521 ttgtagaggg ttcggcgccc acccgagccg cgaagcggcg gctatgtagt tagcccgggg
    11581 tgcaagcccc ggggcgaatg ccgattttgc aatgacccag gccgcgtagc ggccaccgtc
    11641 tccttcaata caactgatat gccggcggtg gcgactcgaa atccggcttg cgcgcgatca
    11701 acaacagcgc tccgatccgc ttgaaatccc gcaactcctc ggcattctta aaccaccgcc
    11761 cgctgtactc ctccggagtc ggcgtgagat cggaaacccg caccgcccgc acatcctcaa
    11821 acgtgtggcg ctgcagcatc tcaatgtatt cctgttcaga atgtacttgc accggaacct
    11881 tcaactcctc cacccagcgc agcgagtagt ggttgtcgcg atacaagtta atgaggatgt
    11941 acagttcccc cttcggtgcc atcacgcgaa acatttccgc cagcgcgcgt tcctggtcgg
    12001 cgtaatagta aaacgactcg accgacagca ctttgtcgaa aaagttctct tcccacggca
    12061 cttgctgcgc cgatcccact acgaacataa cgttgtcgta atcggtcgaa gcagcacgcg
    12121 cccgcctgat catctcgtcc gacacatcga cgccgatcac ttggcccggt cgcgccgcat
    12181 cgtcaaccgc cttcgccatc aaccggctcg cccatcctgc tccgcagccc aagtcgagca
    12241 cgcgatcgcc cgccttcagc ttgatcagtg ccagcgtttg ctcggtgatg ctgatgtggt
    12301 gagcttccat ctcttcgccc ttgccctgtt cagcccaccg gttaaattcc tggcgtaatt
    12361 gctcgtcagc ggatccagtt ctacggtcgc tcaacgttcc ctcgcggatt tccaacctct
    12421 atgctacagt ctcggaaact catggctcaa actcccgacg aaccctccaa cggttccacc
    12481 aagctcccat cgccactgta ttgccccaat tgcgcgtgtg aagtgaacga tccgctcacc
    12541 tgcggcgact gttccgccgt gatctgccgg gtctgcggaa ctccgctcga gatggctgaa
    12601 gacttgggga tgggctgatc atgcctcaat ccgtccacgc ttccaccgaa cacagcactc
    12661 atcacgaacc cacgctggtt cgcggactct cgctcatgga ttccgtcatg ctgatggtcg
    12721 gcggcatcat cggctcgggc atcttcctca ccgccggaca aatcgccact gccgttcaca
    12781 agccgctcta ctttctgggc gtgtggtttg tcggcggact catctcgctt ctcgcctgct
    12841 tcgccttcgc cgaaatggcc gccatgtatc cccaatcagg aggccagtat gtctacctgc
    12901 gcgaagccta tggcgaattc cccgcattcc tctacggctg gatgatcttc accgtcggcc
    12961 agagcggcac tatcgctgcg ctcgccgtag gattcgccga atacttcggc gccatcgttc
    13021 ctctcgccgc tgcccacaaa cccatcgcaa cgtttctcgg cttcacgcta acgcgcggac
    13081 accttgtctc catcggcgca atcgtcctac ttaccgccgt caatgttgtc ggcgtgaagc
    13141 ggggctccgc gctgatcaac accgcgacct gggccaagtt cgccgccatc ggcatattcg
    13201 ttctgctcgg cctcgctgtc ggcaaaggct cgtggtcgaa ctacgcggtc aatcttccgc
    13261 tcgatcagcc cgcgtcgctg tcgcatgtga tctcaggctt cggagtcgca ctgatcgccg
    13321 tgttctgggc ttacgacgga tgggtttatg taacgtgggt ttcgggcgag atcaaaaatc
    13381 cgcaacgagt tctgccacga gcattattgc tggggattct gctggttggc gtgatctatg
    13441 tcagcgtaaa tgtgatttat ctttatgcgc taccgatcac gcaggtcgcg aacgaaacca
    13501 ccatcgcgca ggccgcagcc gtgacacttt tttcttccaa cgccgccaaa tggctgggcg
    13561 cgaccatcgc gctctcgtgt tttggcgcgg cttccagcgc catcctttgc agcgcgcgtg
    13621 tgtcctatgc gatggcgcgc gacggagctt tttttcatgc catgggacgc gttcatccca
    13681 aataccgcac gcccgcgttc agcctgatcg cgctgagtgc ctggtccgtc gtgttggcgc
    13741 tcagcggcag ctacgaccag ctctacacct acgtcatgtt catgatggtg ctgtcctacg
    13801 ttgcggccgt cgccgcgtta tttgttctgc gccgcaagcg tcccgacgta cctcgcccgt
    13861 atctgtgcac cggttatccc tatctgcccg cgctctacct tctgatggca ggagcgtggg
    13921 cgctgaactc cgtggtcact cgacccaagg agacgctagt cggaatcggc atcgtcttcc
    13981 tcggcgcgcc gttctacgcc ttctggcggt ggcaactgaa acgccatccg gaggagctga
    14041 aggagttcga gtagatgatt cggctaggag tctggcggga atagattctg cagaagcacc
    14101 aaacataata acttttccaa cgggcgtatg gggttaagat gcggccatgg gaaagtgctt
    14161 tcgctccgcg gatgtgaacc aggcgctgct gctgccgcct tcg
//
LOCUS       DASRKK010000766         5936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1353991, whole genome shotgun sequence.
ACCESSION   DASRKK010000766 DASRKK010000000
VERSION     DASRKK010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353991"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFC15_18420"
     CDS             complement(<1..880)
                     /locus_tag="VFC15_18420"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM12190.1"
                     /translation="MDLYALTRKLVDIESITSNEVEVGDVIAGELTAMGYNVERMPVE
                     GDRFNVWATQPDQPRPELVFSTHMDTVPPFIPSSETPERIYGRGSCDAKGIIVAQIAA
                     AEKLKSDGIHVGLLFLVGEERDSQGALAANQNPRGSKFLINGEPTENRIALASKGTLR
                     LEVIATGRMAHSAYPELGESAIEKLLDAVENIRKMKLPVHPTAGPSTLNIGLIEGGRA
                     PNVIPDHASANLLIRLVGPAEELRRQIAAAVAGKAEANFILEIPFMELGSVPGIETMI
                     AAFTTDIPALPNWGKPV"
     gene            complement(959..1507)
                     /locus_tag="VFC15_18425"
     CDS             complement(959..1507)
                     /locus_tag="VFC15_18425"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM12191.1"
                     /translation="MSQRLHSEQWIPVPVPRVFAFFSDPHNLPRIMPPSQGAKLIKFN
                     LVPPRLPEGARPQGLEHMAGPGTEITFKFRAIPYLPIHERWTALITEFAFNEYFADTQ
                     KQGPFKSWHHTHTFESKVVDGRDGTVVGDEVEYEVGFGVIGKTIETLIFQRVMRSIFE
                     HRKQALERVFASEINESRVAKT"
     gene            2097..2534
                     /locus_tag="VFC15_18430"
     CDS             2097..2534
                     /locus_tag="VFC15_18430"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12192.1"
                     /translation="MFRGSHLTRLDDKGRLKMPAEYKRLADEKYGSKFYITSWDGKSA
                     YVYPIQEWETIERKLVGLSTFNASKQKLLKRTSYYGQEVEFDGQGRLLLPAVLRESAD
                     LKGEVSVAGRLTYLEVQNAEALREEVAQPFTDEDAKTLDGLGI"
     gene            2576..3541
                     /gene="rsmH"
                     /locus_tag="VFC15_18435"
     CDS             2576..3541
                     /gene="rsmH"
                     /locus_tag="VFC15_18435"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZM12193.1"
                     /translation="MDNEFPRADSAPGREHGEAGKRQFGHVPVLLQPAIEFLAVRRGG
                     TFIDLTLGLGGHGWEIAKRLGAAGRLIGFDKDPAALGRARERLMNPTQEMAADWPKIE
                     LVHASFAEVAQHVAPASVDGLLADLGVSSLQLDNAVRGFSFQAEGPLDMRMNTQSGQT
                     AEQVVNRFDERELADAIYEFGEERRSRRIARAIVRARPIRTTAHLAEVVSAAARPMKQ
                     AERRIHPATKTFQALRILVNRELDDLQTLLSKGAAPQVLKKGGRLVIISFHSLEDRIV
                     KDALREGAHDGTYRVLTKKPVTAEQEEIDRNPRSRSAKLRAAERI"
     gene            3730..4224
                     /locus_tag="VFC15_18440"
     CDS             3730..4224
                     /locus_tag="VFC15_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12194.1"
                     /translation="MAAGILYPTAADAGSWGDQVSETRGMFMGTPEIYFTKRIDNSRV
                     VRMVDPRRRREMKSFGVTLAVFFLLVMVYLLQHLSAIEYGYRIEQAKAEQTSIVESNR
                     ELKLEESSLKDLERIDIEARKLGMIPPVAGQIQTIESGNGDSAVPVMAKANPSGIMVV
                     SMLQ"
     gene            4390..>5936
                     /locus_tag="VFC15_18445"
     CDS             4390..>5936
                     /locus_tag="VFC15_18445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZM12195.1"
                     /translation="MRASAPAKTPSKRLYILAAVLFLWVAAVLGRLVYLQVIKYQFFL
                     NLASRQHGRTIDVDPRRGTIYDRNGAELAMSIDVDSVFAVPSEIPDQETTAQILAKVL
                     DLDAQELLSHLRAQKNFAWVKRKLDAETSDRVRNLNLRGIYFRKEPMRFYPKRDLAAQ
                     TIGYVGVDDEGLGGIELKFDDDLRGMPGREMIFVDARRKWYGRVERQPDPGQNLVLTL
                     DSTIQYIAEKELAAAMEETKAVAGTVVVQNPRTGEILALVNRPTFNPNVFNSVPKEAL
                     KNRAVSDVYEPGSVFKTVTYSAAIEQKVVTPEDHVDCQGGSITIFGMTIHDAHKMGVL
                     TIAEAYAHSSDVAAVKTGMKLGDVRFDEYIRSYGFGAQTGIELPGESRGLKKPLNRWS
                     KVSIGAMSMGQEIGVTAVQVVSMVSTIANDGVYTPPRIVAGELPPNAAPKPVVFHPAQ
                     QHRVISTMTAVQMKKMMEAVVLDGTARRAILDGYTVAGKTGTAQKVDPATGAYSKTKY
                     VASFVGFA"
BASE COUNT         1295 a         1590 c         1877 g         1174 t
ORIGIN      
        1 gaacaggctt gccccagttg gggagagcgg gaatgtcggt ggtgaaggct gcgatcatgg
       61 tttcgatgcc cggcacggaa ccgagctcca tgaacggaat ctcgaggatg aaatttgctt
      121 cggccttgcc ggctacggca gcggcgatct gtcggcgcaa ctcctcggcc gggccgacca
      181 accggatcag caggttcgcg ctggcgtgat cggggatgac gttaggagcg cggcctccct
      241 cgatgaggcc gatgttcagc gtcgaaggcc cggctgtggg gtgcacaggc agcttcatct
      301 tacgaatgtt ctcgactgcg tcgagaagct tttcaatcgc cgactcgcct aactcgggat
      361 aggccgagtg cgccatcctg ccagtagcaa tgacttccag gcgcagggtt cccttcgacg
      421 cgagggcgat acggttttct gtcggttcgc cgttgataag gaactttgac ccgcgaggat
      481 tctggttggc agcgagcgct ccctgactgt cgcgctcttc gccgacgaga aataacagtc
      541 cgacgtggat gccatcggac ttaagcttct cggcagccgc gatctgcgcc acgatgatgc
      601 ccttggcgtc gcaggagccg cgtccgtaga tgcgctctgg cgtttccgaa gatgggatga
      661 acggaggaac ggtgtccatg tgggtcgaga acaccaactc gggtcgcggt tggtcgggtt
      721 gagttgccca cacgttgaag cggtcgccct cgaccggcat gcgctcgacg ttgtagccca
      781 ttgcagtcag ttcgccggct atgacgtcgc ccacctcaac ttcgttgctg gttattgatt
      841 cgatgtcgac gagcttgcga gtgagggcgt aaaggtccat gtccaaagaa tagccgattg
      901 ccgaaattca ccacagagac acagaggcac ggaggaagtt cgatctcgct ggttcgaact
      961 acgtcttcgc cacgcgcgac tcgttgattt cggaagcgaa gactctttcc agcgcttgtt
     1021 tgcggtgctc gaagatggaa cgcatcacgc gctgaaagat cagcgtctcg atcgtttttc
     1081 cgatcacgcc gaagccgact tcgtattcca cttcatcgcc aacaaccgtg ccgtcgcggc
     1141 cgtccacgac cttgctttcg aaggtgtgcg tgtgatgcca cgacttgaat ggcccctgct
     1201 tctgcgtatc ggcgaaatat tcgttgaatg caaactcggt gatcaaagct gtccatcgct
     1261 catgaatggg taaatacgga atggcgcgga acttgaaggt gatttcggtg ccaggtcccg
     1321 ccatgtgctc aaggccttgt ggtcgcgctc cctccggcaa tcgcggtggc accagattga
     1381 acttgatgag tttcgcgccc tgcgatggag gcatgatgcg cggcagattg tgcgggtcgg
     1441 agaaaaacgc aaacaccctg ggcacgggaa cgggaatcca ctgctcgctg tggaggcgct
     1501 gactcatggc gttgagcgac gcaccgaata tactgcgcga cccagcggat gccaaatttt
     1561 taggctgacg accggccagt gaaagagtca ggaaagcaga tccctcggag actgaagccc
     1621 cgtcgtgatg acaatagtta agggcctgca tcatgcagcc aaagctgcga cccctttcct
     1681 tattctggcg ctgtcaatcg cctcgactgt cggttttgtg ccgacaactt cgggtttctt
     1741 ccggaattga cccgacgcgg taggcgggga atcgccgcgg taaacgcaaa atgctgtggg
     1801 agggggggcg agtgagccca acctgtcgta gtcacgcaga tcctagaaaa ctttcaacaa
     1861 aaaatgcaga tttccacttg acacgaaata gaaagggcgt aagattcagc ccaagtggta
     1921 caaagtggga gggaatggac tagtgaatac ctcccgtgga agctgctaga gcgaattggg
     1981 tattttggcc aaacgcgcga tgcaaccgga ttctagctcc cacgtggggt ggtttttcag
     2041 gttttcctcc tctcccggaa acaaattagg gtcccggcgg tatgggcccg gtatttatgt
     2101 ttcgtggttc tcacttaacc cggctggacg acaaagggcg tctcaagatg cccgcagaat
     2161 acaagcgcct ggccgacgag aagtacggaa gcaaattcta catcacaagc tgggatggga
     2221 agagtgccta tgtatatccc atccaggagt gggagacgat cgagcgcaag ctggtgggtc
     2281 tgtccacctt caatgcatcg aagcaaaaac tgttgaagcg gacgagttat tacgggcagg
     2341 aagtggagtt cgatggccaa gggcgtctgc tgctgccggc ggtgttacgc gagtcggccg
     2401 acctgaaggg cgaggtctca gtggcgggac gtttgacgta cctcgaagta cagaatgcgg
     2461 aagcgcttcg cgaagaagtg gctcagccgt tcaccgatga ggatgcgaaa accctggacg
     2521 gtctgggcat ttagtgaggc agagcaatcc attcaacgtc tcggaacggg agtgaatgga
     2581 caacgagttt ccaagagcgg acagcgcccc cgggcgcgaa catggcgaag cgggaaaaag
     2641 gcagtttggc catgtaccgg ttcttttaca accagcgatc gaattcctgg ccgtgcggcg
     2701 cggcgggacc tttatcgatc tgaccctcgg cttaggcgga cacggttggg aaatcgcaaa
     2761 acgcctgggc gcagcgggtc gtttgattgg cttcgataaa gacccggcag cgctgggacg
     2821 ggcccgtgaa cggctgatga acccgactca ggagatggcg gccgattggc caaagatcga
     2881 gttggtgcac gcgagttttg cagaagtagc gcagcacgta gcgcccgcca gcgtcgatgg
     2941 attattggcc gacctgggcg tcagctcttt gcagttggac aacgccgtgc gcggatttag
     3001 ttttcaggcg gaaggcccct tggatatgcg gatgaatacg cagtccgggc agaccgccga
     3061 acaagtggta aaccgcttcg atgagcgcga gcttgccgat gcgatttacg aattcggtga
     3121 ggaaaggagg tcgcggagaa tcgccagagc cattgttcgg gcgcggccga ttcgtaccac
     3181 cgcacatctt gcggaggtgg tatcggctgc ggcccggcca atgaagcagg ctgagcggcg
     3241 cattcatccg gcgacgaaga cctttcaggc tctccgaatt ctggtgaacc gtgaacttga
     3301 cgacctgcag acgctgttga gtaagggcgc ggcgccgcag gtcctgaaga agggcggcag
     3361 gttggtgatc atcagcttcc attcgctgga agaccgcatc gtgaaagatg cgctgcgcga
     3421 gggagcgcac gacgggacct accgcgtgct gaccaagaag ccggtgaccg ctgaacagga
     3481 agagatcgat cgcaatccgc ggtcacgcag cgcaaagctg cgggccgcag agcggatttg
     3541 agttttgcgg gaagtagata gtcgatgggt tgcgagactt atcaacagtt tcctcaggta
     3601 tatccacaac gcctgcggaa aaggttgtgg gaaatgtgaa taagttgtag tccgacagaa
     3661 ttggactgcg acattgtggg agtttgccgg cgatgcgcca ggtataaggc cgcccggcag
     3721 gagaaagcga tggcagccgg aattctttat ccaaccgcag ccgatgcggg cagctggggc
     3781 gatcaagtat cggagacccg tggcatgttc atgggcacgc ctgagatcta tttcaccaag
     3841 cgcatcgaca attcacgcgt ggtgcgcatg gtcgatccca ggcggcgccg cgaaatgaag
     3901 tcgttcggcg tgacgctggc tgtcttcttt ctgctggtga tggtgtacct gctgcagcac
     3961 ctcagcgcta ttgaatacgg ctatcgcatc gagcaggcga aggcagagca gacatcgatc
     4021 gtcgaaagca accgcgaatt gaagctggaa gaatcgtcgc tgaaggacct ggagcggatc
     4081 gatatcgagg cgcgcaaact ggggatgata ccgccggttg ccggacagat tcaaaccatc
     4141 gagagcggga acggggacag cgctgtgcca gtgatggcga aagcgaatcc cagcgggatc
     4201 atggttgttt cgatgctgca gtagcctccg gtcacagcgc gacgggctga atccggatga
     4261 gagttgagag tagcggtccg cccaggtcgg accgctttct gcatgtcagt accacagtgt
     4321 aagagctgta ccacagagta agactgcgat tcggcggctc cgggatcgcg cttatgggga
     4381 gagcggggga tgagggcaag cgcaccggcg aagacgccaa gcaagaggct gtacatcctt
     4441 gcggccgtgc tcttcctgtg ggtggcggca gtgctcgggc gcctggttta cctgcaagtc
     4501 atcaagtacc agtttttcct gaatctcgcc tcgcgccagc acggacgcac cattgacgtc
     4561 gatccgcggc gaggcaccat ctacgaccgc aacggcgccg aactggcgat gtcgattgat
     4621 gtcgattcgg tgttcgccgt gcccagtgag atccccgacc aggaaacgac cgcgcaaatc
     4681 ctagccaagg tattggatct cgatgcccag gaactgctgt cgcatctccg cgcacagaag
     4741 aatttcgcgt gggtgaaacg caaactcgat gccgaaacca gcgaccgcgt ccgaaacctg
     4801 aaccttcgtg ggatctactt ccgcaaggag cccatgcgct tttatcccaa gcgcgacttg
     4861 gcggctcaga ccattggcta tgtgggagtt gacgatgaag ggctgggcgg cattgaactg
     4921 aagttcgacg acgatctgcg cggcatgcca ggccgcgaaa tgattttcgt cgatgcgaga
     4981 cgcaagtggt acgggcgcgt ggagcggcaa cccgatcccg gacagaacct ggtgctgacg
     5041 ctcgactcca ccatccagta catcgcggag aaagaactcg cggcggcgat ggaggagact
     5101 aaggccgtag cgggcacagt ggttgtgcag aatccgcgga ccggcgagat tctggcgctg
     5161 gtcaaccgcc cgacgttcaa tcccaacgtc ttcaacagtg ttcccaagga agcgctcaag
     5221 aaccgtgcag tcagcgatgt gtacgagccc gggtcggtct tcaagacggt gacgtactcg
     5281 gcggcaattg agcagaaagt agtgacaccg gaagaccatg tcgattgcca gggcggctcc
     5341 atcaccatct tcggcatgac catacatgat gcccataaga tgggagtgct gacgattgcc
     5401 gaggcttatg cacactccag tgacgttgcg gccgtcaaga caggaatgaa gttgggcgac
     5461 gttcgcttcg atgagtacat ccgttcctac ggctttggag cgcagaccgg catcgagctc
     5521 ccgggcgaga gccggggctt gaagaaaccc ctcaatcgct ggtcgaaggt gtcgatcggc
     5581 gccatgtcga tggggcagga gattggtgtc acggccgtgc aggtggtctc catggtttcg
     5641 accatcgcca atgatggcgt ctacacgccg ccgcgcattg tggccgggga gttgcccccc
     5701 aacgccgcac caaagccggt ggtgtttcat cccgcgcagc aacaccgcgt catttcgact
     5761 atgaccgcgg tacagatgaa gaaaatgatg gaagccgtcg tactcgacgg caccgcgcgc
     5821 cgcgccattc tcgatggata caccgttgcc ggcaagaccg gcaccgcaca aaaagtcgat
     5881 ccggccacag gtgcctattc caagacgaag tatgtggctt cgttcgtggg cttcgc
//
LOCUS       DASRKK010000767         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_571665, whole genome shotgun sequence.
ACCESSION   DASRKK010000767 DASRKK010000000
VERSION     DASRKK010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571665"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            612..2210
                     /locus_tag="VFC15_18450"
     CDS             612..2210
                     /locus_tag="VFC15_18450"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM12196.1"
                     /translation="MDAEAQGILLEIPEQPAPAPVPPVAGAVPAAKPKLKPIDREQGL
                     LRPVIVEELVAPDHKCRAIWDLTGQLDLNRFYNKIKSQEGKAGSPAWNPRLLLSVWLY
                     AYSEQVNSAREVARLMEYEPGLMWLSGLGEVNYHKLSEFRTEYPEELKQLMAELLGVL
                     SREGIVKLECVAHDGTKIQAQAGSDTFRRGATLEKEIAKARQMVEELDGQPETGPAEN
                     PRREAARQRAARERSERMKQAAEELEKIRAGKNGEKERKEARVSLTEPEARIMKHGND
                     GGLAPSYNVQISVDAEQKIIVGIELTQSSSDSGSLQPAMEQVQETMGRYPDQVVADGG
                     FTNQASITEMNQSPMDFYGSLAEAEVRQAASMKAAGIDPAFGPNAFRVQAESKTLQCP
                     AGKILEYVRQSKKGDTTYWQYQAEGQDCRGCGYQKQCCPRPEQGRLVSIRMTENVEVA
                     AFRKKMKTEAAQDIYKKRGPVAEFPNAWIKEKLGIRKFRLRGMGKASTEARWGAFTYN
                     VQQWIRLSWRPKLMAGARTVTATA"
     gene            complement(2492..>2801)
                     /locus_tag="VFC15_18455"
     CDS             complement(2492..>2801)
                     /locus_tag="VFC15_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM12197.1"
                     /translation="VVPAFYRRRHRIYLIQDNASYHKKQETYDWFKSNRRYVEVFQLP
                     PYWPELNATERIWNYTRKYVTHNRFFEKPQDLCAALFSRFDYVRHHPQEIEDLLNPFF
                     "
BASE COUNT          641 a          689 c          941 g          530 t
ORIGIN      
        1 tgcatgtgct tagctggtct tgacccctct tccaccgcct cgctggaaaa gtcgatctcc
       61 cgcatgggcg gtgtccccga tgctatagca acggaggccc tggggtcaag gacgtctcat
      121 ttacgtgccc cctcgccgcg cgcgacgatt tttgtgacgc tttgtcccac tagactctca
      181 agccttcaca ggctgcggcg atttcgtggg gcagaccatc gtttttctgt ggtctgccaa
      241 ctgccgcgtc gattcttgcg gctgcccacc tttggttgcg gctccgctgt tctgtgcggc
      301 agattttcaa tctgtttcgg atgtgcgcgg gacgcacagg gggagggatg aaaatcgtcg
      361 cttcgcaggc tctcgatttt tcaatgagtt gcgggcgact ttcgagagtg cggcggattt
      421 gcaatccgcc tggcgcgccg gcaaacaacc gctccggaac taaacgcggc gacgttattg
      481 tttgcgcggc aaccgccttc tcacgcgcgc ggctccgaac agagccgcga ccgtcagaag
      541 gtgtgaggaa ttctgggctg gggcgttcca aaacatttgt aagggtaaat gttttagctg
      601 ataattgagc aatggacgcc gaggcccaag gaatattgct agagatcccc gaacaacctg
      661 ctccagcgcc ggtgccgccg gtagctgggg cggtccccgc cgccaagccg aagctgaagc
      721 caatcgaccg cgagcaaggg ctcttgcggc cagtgattgt ggaggaattg gtggcgccgg
      781 atcacaagtg ccgagccatt tgggacctca cggggcaatt ggatctaaac cgcttttaca
      841 acaagatcaa gagccaggaa ggcaaggctg ggtctccggc gtggaacccg cggttgctgc
      901 tgagcgtgtg gctctatgcg tacagcgaac aggtcaactc ggcgcgggag gtggcacgac
      961 tgatggaata cgagcccggc ctgatgtggc tgtcgggttt gggagaagtg aactatcaca
     1021 agctgtcgga gtttaggacg gaatacccgg aagaattgaa gcagttgatg gccgaattgc
     1081 tgggagtgct ctcgcgggaa ggaatcgtca agctggagtg cgtggcgcac gacggcacga
     1141 agatccaggc ccaagcggga agcgacacgt tccggcgggg agcgacgtta gagaaagaaa
     1201 tcgccaaggc gcggcagatg gtggaggagt tggacggaca accggaaacc ggaccagcgg
     1261 aaaatccacg gcgggaagcc gcgcggcagc gtgcggcgcg ggaacgcagc gaacgcatga
     1321 agcaagcagc cgaggagttg gagaagatcc gggcgggcaa gaacggggag aaggagcgga
     1381 aggaagcgcg agtgagtctg accgagccgg aagcgcggat tatgaagcac ggcaatgacg
     1441 gcggcctagc gcccagctac aacgtgcaga ttagtgtcga tgccgaacaa aagatcatcg
     1501 tggggatcga gttgacgcag tccagcagcg acagtggatc gttgcagccg gcgatggagc
     1561 aggtgcagga aacgatgggg cggtatccgg accaggtggt agcggatgga ggattcacca
     1621 atcaagcttc gattacggag atgaaccaga gcccgatgga tttttatgga tcgctggcgg
     1681 aagcggaagt gaggcaggca gcttcgatga aagcggcggg aatcgatccg gcgtttgggc
     1741 cgaatgcgtt tcgagttcag gcagagagca agaccttgca gtgcccggcg ggaaagatct
     1801 tggagtacgt gaggcagtcc aagaaaggcg acacgacgta ctggcagtat caggccgaag
     1861 gccaggattg cagaggatgt ggttatcaaa aacagtgttg tcctcgaccg gaacaagggc
     1921 ggctggtatc catccgcatg acggagaatg tggaggtcgc ggcgttccga aagaagatga
     1981 agaccgaggc ggcgcaggac atctacaaga agcggggacc ggtggcggag tttcccaatg
     2041 cctggatcaa ggagaagtta gggattcgga aattccggct gcgggggatg ggcaaggcga
     2101 gcacggaagc acggtgggga gcgttcacct acaacgtgca gcaatggatc cgattgagtt
     2161 ggcggccgaa gctgatggct ggcgcaagga cagtcacggc gacggcatag cgaaggcggg
     2221 gaagagggcg aagctgggct tggaagggct gagcgggccc gaaagtggtg ccatgtcaag
     2281 gagattggac ggtgacggtc gaatctcaat actctacact acaaagtacc cgtcggcgca
     2341 atgccacatt aggcccgtca cccaatttac gacgtccacc ctggtgcgat ggttttcagt
     2401 caacggcctt cggaatttcg atttcctcac ggcttctcag agagcggtct ttactgctat
     2461 atatccgcgc ataaataatt cgacatcaca atcaaaaaaa gggattcagc aggtcttcga
     2521 tctcctgcgg atggtgtcgc acgtagtcaa atctgctgaa gagcgcggca cacagatcct
     2581 gcggtttttc gaagaaccga ttgtgagtga cgtacttgcg cgtgtaattc cagatgcgct
     2641 cggtcgcatt caattccggc cagtagggcg gaagctggaa gacctccacg tagcggcggt
     2701 tggatttgaa ccagtcatag gtttcctgct tcttgtgata cgatgcgttg tcttggatca
     2761 ggtagatgcg gtggcgacgg cggtaaaagg cgggcacgac c
//
LOCUS       DASRKK010000768         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1061036, whole genome shotgun sequence.
ACCESSION   DASRKK010000768 DASRKK010000000
VERSION     DASRKK010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061036"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="hpnA"
                     /locus_tag="VFC15_18460"
     CDS             complement(<1..890)
                     /gene="hpnA"
                     /locus_tag="VFC15_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZM12198.1"
                     /translation="MGSHVARQLLSQGAQLRLLVRPTSRIDNIADLPAERVVGDLRDL
                     ESLKKGMSGCEFVFHVAADYRLWTRNGQELYDSNVEGTRNVLAAASDSGIRRVVYTSS
                     VATMGFGNNGRLTDEKTPVTLSNMIGDYKRSKFMAEQLVIEAGRGGQNVVIVNPTTPI
                     GERDIKPTPTGRIVVDFLRRKFPAYVDTGLNLVDVADCALGHLLAMEKAVPGERYILG
                     GENLTLKQILDKLAAITGLPSPKLKLPYAVAYATGVVDTVVSGKILKREPRVTLDAVR
                     MGRKKMFVTSAKAERELGWNP"
     gene            complement(1046..1345)
                     /locus_tag="VFC15_18465"
     CDS             complement(1046..1345)
                     /locus_tag="VFC15_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12199.1"
                     /translation="MAVVLSATLKNWLYWPGGAATMTITLDIKPELEAELTAQAAARG
                     MDVPAYAAILLEQAAQPTERQQRKQTLSEFLMESPLAGSGIVIERDKDPGRDIDF"
     gene            complement(1403..>2066)
                     /locus_tag="VFC15_18470"
     CDS             complement(1403..>2066)
                     /locus_tag="VFC15_18470"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZM12200.1"
                     /translation="NNTRMVFQNIPFADHNAMLDPPTVDITGRVLECLASYGYSLKDE
                     PVRRALEFIKREQAIDGSWFGRWGVNYIYGTMQVLRGLEAVGVDYHETFVLQAVEWLR
                     MMQNPDGGWGETCGSYDDPNTKGVGPSTPSQTAWAVMGLLAANDTRNDSLQHGIAYLL
                     KTQSRDGSWDETLYTGTGFPRVFYLMYHMYRQYFPLLALTTYAKASAASQEEQLRVAR
                     RD"
BASE COUNT          409 a          648 c          588 g          421 t
ORIGIN      
        1 ggattccagc ccagctcgcg ctccgccttc gcgctggtga caaacatctt cttgcgtccc
       61 atgcggaccg cgtccaaggt cacgcgaggt tcgcgcttga gaatcttgcc ggatacgacc
      121 gtgtccacca cgccagtggc ataggccacc gcgtaaggca acttcaactt tggtgaaggc
      181 agacccgtaa tcgccgccaa tttatcgagg atctgcttca atgtcaggtt ctcaccaccg
      241 aggatatagc gctcacccgg cacggctttt tccatcgcga gcaagtgtcc tagagcgcaa
      301 tccgcgacgt ccaccaaatt cagccccgta tccacatagg ccgggaattt acgccgcaga
      361 aagtcaacca cgatccggcc ggtgggcgtc ggcttgatat cgcgctcgcc gataggagtc
      421 gtcggattca cgatcacaac gttttgtccg ccgcgtcccg cctcgatcac cagctgttcc
      481 gccatgaatt tgctgcgctt gtaatcgcca atcatgttgg aaagcgtgac cggcgttttc
      541 tcgtcggtaa ggcggccgtt gttgccgaag cccatggtgg caacgctgct ggtataaaca
      601 actcgccgga tgcccgaatc actcgcagcg gcgaggacgt tccgggtgcc ttcaacgttg
      661 gagtcgtaga gttcctgtcc gttacgcgtc cagagccgat agtccgcggc tacatggaag
      721 acaaactcgc aacccgacat gcctttcttc agcgattcca aatcccgcag atcgccaacg
      781 acgcgctccg ccggcaggtc ggcaatgttg tcgatacggc tggtgggacg caccagcagc
      841 cgcaactgcg cgccttggga aagcagttga cgggcaacgt ggcttcccaa aaatcctgtc
      901 gctcctgtga caaaacattt catctgtcag ttggcagtag tcagttggaa gtagtcagcg
      961 ggcccacagt ggctaaccca accggtcgtc attcgcaggg tgccccaccc tcctcgcccg
     1021 cctttggcga gagggtggaa accgctcaaa aatcaatatc ccggccggga tccttatcgc
     1081 gctctataac aatcccggaa ccggccaggg gcgactccat cagaaactcg gagagagttt
     1141 gtttgcgctg ttgacgctcg gtcggctgcg ccgcctgttc cagcaggatg gcggcatagg
     1201 ccggcacgtc catgccgcga gcggcggcct gggcagtcag ttcggcctcc aactcaggct
     1261 tgatgtcgag cgtgatcgtc atcgtcgcag ctccccctgg ccagtatagc cagtttttca
     1321 gggttgcaga aagcacgact gccacaacct tctcaccggt ttttggcgag aaggtgggag
     1381 cctgtcttgg tcaatggcta cgctagtctc tgcgcgccac ccgcaactgc tcctcttgcg
     1441 aggccgctga cgctttggcg tacgtggtca gcgcaagcaa cgggaagtac tgccggtaca
     1501 tgtggtacat cagatagaac acgcgaggaa atcccgtgcc ggtatagagc gtctcatccc
     1561 acgacccatc ccggctctgc gtcttcagca gataggcgat accgtgctga agcgaatcgt
     1621 tgcgggtgtc attggcggcg agcaaaccca tcaccgccca cgcagtctgg gaaggcgtgc
     1681 tcggaccgac ccccttggtg ttgggatcgt cgtatgagcc gcaagtctcg ccccaaccgc
     1741 catcgggatt ctgcatcatg cgcagccact cgacggcctg cagtacgaag gtttcgtgat
     1801 aatccacgcc gaccgcctcc agcccgcgca gcacctgcat ggtgccgtag atgtagttca
     1861 cgccccagcg tccgaaccag gagccatcaa ttgcctgctc acgcttgatg aactcgagcg
     1921 cgcgccgcac cggttcgtcc ttgagcgagt agccatagct cgccaggcat tccagcaccc
     1981 ggccggtgat atctaccgtc ggcggatcaa gcatggcgtt gtggtcggcg aagggaatgt
     2041 tctggaagac catgcgggtg ttgttc
//
LOCUS       DASRKK010000769         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_277616, whole genome shotgun sequence.
ACCESSION   DASRKK010000769 DASRKK010000000
VERSION     DASRKK010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277616"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFC15_18475"
     CDS             complement(<1..276)
                     /locus_tag="VFC15_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12201.1"
                     /translation="MKAKSLLTALTLLGILATVSCNQQQGPVREAISHKNDYQLAYFY
                     GFPMIAAYKAMYEFNVDKSNSQYKGPFNQVLNTAQVFTPKDTAVTTPN"
     gene            complement(369..1052)
                     /locus_tag="VFC15_18480"
     CDS             complement(369..1052)
                     /locus_tag="VFC15_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12202.1"
                     /translation="MPEGGGSNIEIAHHLTERKEESGPQMPRAVEIAEAIVLAIVAIT
                     TAWSGYQAALWTGHQSKLYGLASKQRVQAARAQDSANYERLYNAATVSQWLQAETSGQ
                     KKLADLYERRFLPEFRPAFDAWKKTDPLNNPNAPPGPFHMPEFRSANADEAGKLSAQA
                     SDTFEQGDEARHHADDYVRETVMLATVLLLTAISQRFKIRGVRIGLITVAGTLLCLSI
                     YHIVSLPRV"
     gene            1297..>3158
                     /locus_tag="VFC15_18485"
     CDS             1297..>3158
                     /locus_tag="VFC15_18485"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HZM12203.1"
                     /translation="MKTRVASTVALALVLISVGVLVSCEKPKAPEQAQQAGGTAPAQT
                     EGRVEKNPDRNAYFGEEHIHTSWSMDAWLMGNRITDPDDALKYAQGKTIKHPMGYDIT
                     IDTPMDFMGVTDHSEYVGITAEANTPGSAVSKMPQAQPLILKNPNDQAEIQRAFTYLV
                     GLNSGPPVKAFMSPAVAGSIWKKNVSIADANNHPGKFTAFCSYEWTSMPNYRNLHRNI
                     FFKDCAKVPEMPYSSLDSNHPEDLWKWMDSQRKAGNELLAISHNANLSDGWMYPVDLD
                     SFGRPIDAAWAASRDRNERLVEIKQIKGQSETHPLLSPNDEFASYELFQDGLLGQPPT
                     GGRIDHIQGSFARQAYKDGITMQDVRGYNPYKFGMAGGSDSHNTGSPYRQDNFYGGHA
                     QIDGTPDRRLAGVLAFGTLDVRLENPGGLTGVWAEENTRASLWDAMYRKETFGVSGPH
                     IKVRFFGGWEYNKDILNTSDWVHQSYAKGVPMGADLPPLKGTAPTFVVWAVKDPTSGN
                     LDRIQVIKGWTKNGQSFEKIFDVVWSGDRKPDKWSGRVPAIKSTVDLEKATYTNDVGS
                     TELKTVWTDPEFDASLHAFYYARVLEIPTPRWTLIQAVKAGLTPPDVVPLTGQER"
BASE COUNT          654 a          940 c          922 g          642 t
ORIGIN      
        1 gttaggtgtg gttacagctg tgtccttggg tgtgaagacc tgcgccgtgt tcaggacttg
       61 attgaatggg cctttatact gcgagttcga cttgtccacg ttgaactcgt acatggcttt
      121 gtatgcggcg atcatcggaa atccatagaa gtaggccagc tgatagtcgt ttttgtgcga
      181 aatcgcctcg cgcaccgggc cttgctgctg attgcaggag acagtcgcga ggattcctag
      241 aagagtcagg gccgttagca gcgattttgc tttcatacct tgtgccttcg tagcagcggc
      301 ccgacggagc gagacgcaag tcagaattag gtttacgagc tttggggaca gccctcgcga
      361 gctgcctcct aaactcgcgg aagcgaaaca atatggtaga tcgaaaggca aagcagagtg
      421 ccggccacgg tgatcagccc gatccgtaca ccacggattt tgaatcgctg gctgatggct
      481 gtgagcaaca gcaccgttgc cagcattacg gtttcccgca cgtagtcgtc ggcatggtgg
      541 cgcgcctcat ccccctgctc gaaggtgtcg gaagcctggg cactcagctt acccgcttcg
      601 tccgcgttgg cgctgcgaaa ttcgggcatg tggaagggcc caggaggagc gttggggttg
      661 ttgagcggat cggtcttctt ccaagcgtca aacgccggac gaaactcagg caggaagcgg
      721 cgctcataca ggtcggcgag tttcttctgc ccgctggttt cagcctgcag ccactgcgat
      781 accgtggcgg cgttgtacaa gcgctcgtag ttggcagagt cttgcgctcg cgcggcctgt
      841 actcgctgtt tgctcgccag gccgtatagc ttcgactgat ggccggtcca gagcgccgcc
      901 tggtatccgc tccatgccgt ggtgatggcc acgatcgcga gcacaatggc ttcggcgatc
      961 tctacggccc ggggcatttg gggcccggat tcctctttgc gttcggtgag gtgatgggct
     1021 atttcgatgt tggaacctcc accttccggc attatgatgt ctccttggcg ctttcgtggg
     1081 agttgggtgg ggacaatttg tcgtcgcaac taatatacgc atccaatgtg gcttgcgcag
     1141 gctctcctgc ttaactagaa tttcctcgca ctgaaaaact cgacaccgca gttagttctg
     1201 cggcgacggt tttaccccat cgcgcactag tgggtaatac ccccgcgtgc gcttcgcgtt
     1261 cgtgtggttg tggcattcag caaggcggag gcgtttatga agacgagagt tgcgtcgacg
     1321 gtagctttgg cgttggtcct catcagcgtc ggcgtattgg tttcgtgcga aaaacccaaa
     1381 gcgcccgagc aggcccagca agctggtgga accgctccgg cacaaaccga ggggcgcgtc
     1441 gaaaagaatc ccgaccgcaa cgcctacttc ggtgaggagc acatccacac cagctggtcc
     1501 atggacgcct ggctcatggg caaccgcatc accgaccctg atgacgctct caagtacgcg
     1561 cagggcaaga ccatcaagca ccccatgggc tacgacatca cgatcgacac gccgatggac
     1621 ttcatgggtg taaccgacca ctcggaatat gtcggtatca cagccgaggc taacactccg
     1681 ggatcggccg tcagcaagat gccccaggca caacctctca tcctgaagaa cccgaatgac
     1741 caggctgaga ttcaacgggc ctttacgtac ctcgtaggcc tgaacagcgg gcctcccgtg
     1801 aaagcgttta tgagccccgc ggtggccggc tccatttgga agaagaacgt cagcatcgcg
     1861 gatgcgaaca accatcccgg caagttcact gcgttctgct catacgagtg gacgtctatg
     1921 ccgaactatc gcaacctgca ccgcaacatc ttctttaaag actgtgccaa agttccggaa
     1981 atgccgtata gctcgctcga ttccaaccat ccggaagatc tctggaagtg gatggactcg
     2041 caacgcaagg cgggcaacga gttgctcgct atttcccaca atgccaacct cagcgatggc
     2101 tggatgtacc ccgtcgacct cgacagcttc ggacgtccca tcgacgctgc ctgggctgcc
     2161 tcgcgcgacc gcaacgaacg cctggttgag atcaagcaga tcaagggaca gtcggaaacc
     2221 caccccttgc tctcgcccaa tgacgagttt gccagttacg agctcttcca ggacggtcta
     2281 ctaggtcagc caccgaccgg cggccgcatc gatcatattc aaggcagctt cgcccgccag
     2341 gcctacaaag acggcatcac catgcaggac gtacgcggct acaacccata caagttcggc
     2401 atggccggcg gctccgattc gcacaatacc ggcagtcctt accgccagga caatttctat
     2461 ggcggccacg ctcagatcga cggaaccccc gaccggcgct tggccggagt tctggcattc
     2521 ggcactctcg atgtacgcct ggaaaacccg ggcggcctca ccggtgtctg ggccgaggaa
     2581 aacacccgcg cctcgctgtg ggacgcgatg taccgcaagg aaaccttcgg cgtcagcggc
     2641 ccgcacatca aggtgcgctt cttcggcgga tgggagtaca acaaagacat cctgaacacc
     2701 agcgattggg tacatcagtc gtatgcgaag ggcgttccca tgggcgccga cctgcctccg
     2761 ctaaagggca cggctccgac gtttgttgtc tgggcggtga aagacccgac ttccggcaat
     2821 cttgaccgca tccaggtcat caagggctgg accaagaacg gccagagctt cgagaagatt
     2881 tttgacgtgg tctggtccgg cgaccgcaag cctgacaagt ggtcaggcag ggttccagcg
     2941 atcaagagca ctgtggacct ggagaaagcc acctacacca acgacgtggg ttcgaccgaa
     3001 ctcaagaccg tctggaccga ccccgagttc gatgccagcc tgcacgcgtt ctactacgct
     3061 cgcgtgctgg aaatccccac gccgcgctgg acgctcattc aggcggtcaa ggccgggttg
     3121 actccgccgg atgtggttcc gctcaccggc caggagcg
//
LOCUS       DASRKK010000770         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_670358, whole genome shotgun sequence.
ACCESSION   DASRKK010000770 DASRKK010000000
VERSION     DASRKK010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670358"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..1741
                     /locus_tag="VFC15_18490"
     CDS             653..1741
                     /locus_tag="VFC15_18490"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZM12204.1"
                     /translation="MPYDDATQFLDTASMQSGVMQTVSPDSAIRDRAQAMVQKVSAVA
                     TALALNQALYHALAALDVSKADRATQYYVQRTLLEFRLAGVDKDDATRAHIKTLNDEI
                     TKYATQFERNLQESQLKVVVKNAAELDGLPEDFIKTHKPAADGTITLTSDSPDVTPVL
                     KFAKSADLRKRIFLAYNNRAYPQNVAVLTDLLKKRDELANLLGYKHWSDLNAADKMAV
                     NAPNIAKFFGEIDVASKPAADREYKMLLAAAQKQDPALQQISFQDRSYYFEQLRRSEF
                     DFNSQAARPYFPYDRVQQGILDVASKLFHVTFRPVKDVVVWDPSVTTFDVYESNKQLG
                     RISYGKKIHLGCKEGKFTRPKAHAASRS"
     gene            complement(1720..1917)
                     /locus_tag="VFC15_18495"
     CDS             complement(1720..1917)
                     /locus_tag="VFC15_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12205.1"
                     /translation="MFGERPLRRALQQYVAHYHEERNHQGKQNRLLFPLPNRGASGEN
                     GIVRCKERLGGLLKYYEREAA"
BASE COUNT          429 a          662 c          583 g          397 t
ORIGIN      
        1 gcgcctacga gcagatgctt gcgattacgg ccggctcgga actgagggtg cgcccctcgc
       61 acgccggaga ggccacagga accgcccggt aaagtgtagc caccccaacc cccatctcgc
      121 gcgagatcgc ccgccaaggg gtgccggaag cgcgcagggc ggcaatctgt gaagggttca
      181 cgccggaggg cggtcgtccg agtcgcttcc ccttggctct ggcgttccgc agtccggccc
      241 ttactcgttc cctgaataag cgaccgttcg aactcggcca tagccccgat gatctgaaac
      301 atgagccgac cggaaggcgt cgagaggtcg aggttgtctc ggaggctcac aaatgcgact
      361 ccgtacgctg cgagatcctc cagcgcgttg actaggtgct tgagtgatcg cccgaagcgg
      421 tctatcttcc aaacgagcac ggcatcgaac ttgcgccggt gagcgtcttt tgtcagctga
      481 ttcaattcag gacgtgactc tttcgagccg gatacaccct gatcgacata ctcgccgact
      541 acggtccacc ctcggtggcc agcatattcg cggagttcgg acaactgcat ttcggggttc
      601 tggccgtgaa gagttgacac ccgtgcgtag atagcaattc gcgttgtcat gtgtgccgta
      661 cgatgatgcc actcagttcc tcgataccgc gagcatgcag tcgggggtga tgcagactgt
      721 gagtcccgac tccgccatac gcgaccgggc gcaggccatg gttcagaaag tgagcgcggt
      781 ggcgacggcg ctggccctca accaggcgct ctatcacgcc ctggcggcgc tcgatgtttc
      841 caaggctgac cgggcgacgc aatactacgt gcagcgcacc ctgctggaat ttcgtctggc
      901 tggtgtggac aaagacgacg ctacgcgcgc ccacatcaag acgctcaacg acgaaatcac
      961 caagtacgcg acccaattcg aacgcaacct gcaggagtcc caacttaagg tcgtggtcaa
     1021 gaacgcggct gaacttgacg gcctgccgga ggacttcatc aagactcaca agccggcagc
     1081 cgacggcacc ataacgctga ccagcgactc tcccgatgtg acgccggtgc tgaagttcgc
     1141 caagagtgct gacctgcgca agcgcatctt cctcgcctac aacaaccgcg cttatccgca
     1201 aaacgtcgcg gtgctgaccg acctgctgaa gaagcgcgac gaactcgcca acctgctcgg
     1261 ctacaagcat tggtccgact tgaacgccgc tgacaagatg gcggtgaatg ctcccaacat
     1321 cgccaagttc ttcggcgaga ttgacgtcgc gtcgaagccc gcggccgacc gtgagtacaa
     1381 gatgctgctg gccgcggccc agaaacagga ccctgcgctg cagcagatct ccttccagga
     1441 ccgcagctat tatttcgagc agcttcgccg cagcgagttc gacttcaact cgcaggcggc
     1501 ccggccctat ttcccctacg accgcgtgca acagggcatt ctcgacgtcg cctccaagct
     1561 cttccacgtg accttccgac cggtgaaaga tgtcgtagtg tgggacccgt cagtcacaac
     1621 gttcgacgtc tacgagagca acaagcaact gggccgcatc tcgtatggta aaaaaattca
     1681 tcttggctgc aaggaaggga agtttacccg accgaaggct catgccgctt cccgctcata
     1741 gtacttcagc agaccaccca gtcgttcctt gcaacgcaca attccgttct ccccgctcgc
     1801 ccctcgattc ggcaatggga acagcagtcg gttctgcttg ccctggtgat tgcgctcctc
     1861 gtggtaatgg gccacatatt gctgcagggc tcggcgcagc ggcctctccc cgaacaagat
     1921 gagcttcgga caggcattcc tgcttcaccg atcttaccca gcgttccgca taggcgttca
     1981 aattcggact cctcgccggt agggccatgg ttttcacccg gccagcctcg atcagttgac
     2041 gaaacgagag gcagaacttg ctgtcccgat c
//
LOCUS       DASRKK010000771        13746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_474444, whole genome shotgun sequence.
ACCESSION   DASRKK010000771 DASRKK010000000
VERSION     DASRKK010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474444"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFC15_18500"
     CDS             complement(<1..588)
                     /locus_tag="VFC15_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12206.1"
                     /translation="MKKLLCVVLWLFPVMLFAQSPFDGTWKTNMAESKLSPKPYVFSI
                     DKGMYDCESCVPKINVKADGQDQPVTGQPYDTLAVQVVDANTLHATGKKAGKPTFEQT
                     RTLSSDGKMLTVSSTNYPADGSQPFKAEAKYARVSKGPAGSSAISGSWRIQNVNEDAA
                     GLTTTWKGTGDGLSMSTPTGENWEAKLDGKEYPVKG"
     gene            complement(821..2365)
                     /locus_tag="VFC15_18505"
     CDS             complement(821..2365)
                     /locus_tag="VFC15_18505"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZM12207.1"
                     /translation="MNLAIGQYFSNADYALALPMLLLALFALGILLIDLILPKEWKPV
                     NAYIALIGVAFSAAAVGKLHSAYRVAELRGHDVPGFTGFMGTLLVDRFAIYFFYLFLV
                     GAAIAILMSIHYLEVEHENHGEFYALILFSVVGMMCMAAGFDIVLLFIGLELMAISTY
                     VLVGFLRRDKRSNEAALKYLLLGAFSSGIFAYGLSLFYGVTGSTNLGVIAQKLQEVMA
                     TRPHDPIVAFALLTTTVGVLFKIAAVPFHQWAPDAYEGAPTSVTGFMSVAVKAAGWAL
                     LLRIFLFGLGPLRSMYVPLLVFVAIVTMTVGNLAAITQNNLKRLLAYSSIAHVGYMLL
                     GLVASDGVNNSTGIMAIMIYLLVYTFMNLGAFAVITSLRRRNIIGDDIDDIAGLYSKA
                     PAETMLMLLFLLSLAGIPPLAGFWGKYFIFLSLIQTGHYILASVAVIYAVISMYYYMR
                     IASAMIIKPAVDAEPVSIGPAMGLALLVTAAGTVGIGIFPNFFINAVTWSLGLVNGSH
                     VAMLMR"
     gene            complement(2376..4082)
                     /locus_tag="VFC15_18510"
     CDS             complement(2376..4082)
                     /locus_tag="VFC15_18510"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZM12208.1"
                     /translation="MYNHILSIVLFTPLVGAVLLLLVPKENKDAIRWIANIFGFLGFA
                     VSLPLVPWFWAVKERAGFQFVEGSPNNWIPSIGAGYVIGIDGISFLLIMLTTLLGAIS
                     ILSSWEAIQDRAKEYYAWFLLLQTGMLGVFMSNDFFLFFVFWEAMLVPMYLLIGIWGG
                     PRKLYAAIKFFLYTLFGSVLMLLGIIFLYFHHHTLTGIYTFSIPELYTTAPKIAGPAA
                     LWLFFAFFLGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFSLPF
                     FPGIVMMPWVRTLMVTLSIIGIIYGALVSLVQKDMKKLVAYSSVSHLGFCTLGIFACS
                     PLGISGSVIQQINHGISTGALFLIVGILYERRHTREISEYGGISTIMPVYATIALIMF
                     MSSMGLPLLNGFVGEFTILQGTFMENKWWAAWAAPGVILAAAYLLWLFQRVFFGPNNN
                     PKNASLKDLSARELATFVPLVILAFAIGLYPKPLFQILATPVNNLVATVRPDYPGLTK
                     PVVAAQPDAATPLPEAPKAPEVKTPTATSPTATGLTQKKSQVPVKVASANPNAFVPAS
                     GK"
     gene            complement(4139..6271)
                     /gene="nuoL"
                     /locus_tag="VFC15_18515"
     CDS             complement(4139..6271)
                     /gene="nuoL"
                     /locus_tag="VFC15_18515"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZM12209.1"
                     /translation="MYFLDHIWLIPLFPAVGAACMFFFGRKLSKSAVNGICVGLVALA
                     FLWACAAVWQYTRYSAANPGKPFEKVMFTWLGTGDSSLSYVTNGGHTAQFKADAGFLL
                     DPLSSIWLLFVTGVGMLIHIYSVGYMAHEGGYYRFFGYLNLFMFSMLTLILANNYALM
                     FVGWEGVGLCSYLLIGFYFLRHSASTAANKAFIVNRIGDAGFLLGMFMIAWHFGSVQY
                     TQVNAMARSGAFSIGDPVITAACLLLFVGACGKSAQFPLYVWLPDAMEGPTPVSALIH
                     AATMVTAGVYMVARSNALFVLAPHAMKTVAIVGALTAIFAATIGLVQNDIKRVLAYST
                     VSQLGYMFLALGVGAFGAGVFHVFTHAFFKALLFLGSGSVIHAMSGEQDMQNMGNLHG
                     KIKTTFGTMLIGTLAIAGIPGFAGFFSKDAILWETWSREAGAYRYLWYIAFLTALMTS
                     FYMFRLIYLTFFGKPRMSHDVEHHIHESPKTMTIPLVILALCSVGAGYLGMPHSLGGS
                     NKFEKFLEPVFAKDARVFVNEGASEQVFAGDKQVEHTDPLEYALMFASVGAAIVGWWL
                     AQRAYKHAEKGYQEPIQEISPRFYKTLFRKYYVDEIYDWVFTGRKKLGPARLGALGLG
                     EGLWTFDANVIDGAVNGAGWSTRMSGTISTWWDKWIIDGLFVNGVAVLTRLASYPVRL
                     IQWGLVQWYALVMVAGLVCFIFYYVVKW"
     gene            complement(6418..6723)
                     /gene="nuoK"
                     /locus_tag="VFC15_18520"
     CDS             complement(6418..6723)
                     /gene="nuoK"
                     /locus_tag="VFC15_18520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZM12210.1"
                     /translation="MGEITTLHYLVVAAALLIIGTIGVLTRRNVVIILMSIELILNAV
                     NLNLVAFSRLYGLHGQIFSIFVITDAAAEAAVGLGIIIAFFRNKETVNVDEAELLRW"
     gene            complement(6725..7237)
                     /locus_tag="VFC15_18525"
     CDS             complement(6725..7237)
                     /locus_tag="VFC15_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZM12211.1"
                     /translation="MNPVATTFFFYFLSIIAVASAIAVITRKNAVHAALSLIVTLVSL
                     AGLYLMLYAPFVAGVQIVLYAGGIMVLFLFVIMLVNLDQNIREIQFNKQWMVGLIAAL
                     SLGGLLLFVIRQGRSIFPITLSATLPEGQNTQQIGLALYGQYMLPFEIASLLLLVAIV
                     GAVVMAKKRI"
     gene            complement(7410..7871)
                     /locus_tag="VFC15_18530"
     CDS             complement(7410..7871)
                     /locus_tag="VFC15_18530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZM12212.1"
                     /translation="MSVAPLLRKIFLVDLLKGMSVTFQNQSEKERITEQYPLERPVIF
                     ERYRGQPRLNTNPDTGESLCIVCNLCALACPEQLITVKGERNSVTKRKEVVSWTYDLS
                     RCMFCGLCAEACSTDALELTQDYEMALYSRDGMVLDQAALERGPEPVRYEK"
     gene            complement(7868..9148)
                     /locus_tag="VFC15_18535"
     CDS             complement(7868..9148)
                     /locus_tag="VFC15_18535"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HZM12213.1"
                     /translation="MIESIKNYLSSAVTPGEAGSQLWATVYIIAIVLGLSLSVIAMTW
                     LERKVLAHMQIRLGPMRVGPHGLLQPFADTLKLLIKEDIVPTNADKVVFWSAPVVVTM
                     TAFTTFLVVPFGRTHAVTDMNIGVLFMLGISSLGVLGIIMAGWASNSHYPLMGSLRSS
                     AQMVSYEIAMGLAIVCAVLMTSLQTGTGTLSMIGIVEAQNAQGMWFIFKFFPLGFIAF
                     IVFAIAMIAETNRAPFDLPEAESELVAGFHTEYSGFRWSLFFLAEYTAMLAVSSIAVT
                     LWLGGWLRPFANLLHGPAWDLAFSLVPALSFLAMGAGCFLGTVRMPKIAQMRAQTIGL
                     AVFGALLVLLGLFLLVPGVRERVQDIFWFVFKVSSFMYLYIWYRGTFPRYRFDQLMKV
                     GWKVLLPIGLVVLIATAVVGLRQELWAAIVGVLQ"
     gene            complement(9148..10299)
                     /locus_tag="VFC15_18540"
     CDS             complement(9148..10299)
                     /locus_tag="VFC15_18540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZM12214.1"
                     /translation="MSEPIDTSYLDLDTKDETFLDSTEMVLNMGPQHPSTHGVLRVIL
                     KLDGEKVLGTECIIGYLHRGVEKIGENRTYTMFNPYVDRMDYVAAVSNGLGYCETVEK
                     LLSVEAPPRAQYIRVILTELCRISSHQVWLGTHALDIGALTPLFYAFRDREEILKIFE
                     KYCGARLTTHAFRIGGCIYETYDGFEQDVKKFCEFFIPKLDEYEELLTTNRIWLTRTK
                     GVGILNPEDCIALGVTGPVLRASGVKWDLRKAQPYESYKDFDFEIPTGVNGDTYDRYL
                     VRVAEMRQSVRILQQAIEAIPAGPIMAKVPKVIKPPVGEVYHSIEAPKGELGYFIVSD
                     GSTQPYRVRVRPPSFVNLQALDKMVRGLLVADVVAVIGTLDIVLGEVDR"
     gene            complement(10296..11048)
                     /locus_tag="VFC15_18545"
     CDS             complement(10296..11048)
                     /locus_tag="VFC15_18545"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZM12215.1"
                     /translation="MPEETKEPDSVLPQPEGAKAPSNADAAGKPAAPAVGAESAKVTP
                     ATEANPVESAKPAAAAKPAVPAAAAAPKPPAAPAKPAGPVPEPWESKFAAQLRQQYGS
                     AIQEASKYLGQTYMVVDQSILSEILRRMRDDEGFDYCVDITAVHYPKREQQFDMVYVL
                     YSFPKNERTRVKTRIKDGEHVPSAVSIWETANWLEREVFDMFGIVFDGHPGLKRILLP
                     DEWKGHPLRKDYDILLQDREWVQANLGIESGQ"
     gene            complement(11310..11666)
                     /locus_tag="VFC15_18550"
     CDS             complement(11310..11666)
                     /locus_tag="VFC15_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZM12216.1"
                     /translation="MPQNYVPIFIFALVAFLVAAGTLALFTLIRPRKPNTIKLMPYEC
                     GITPFDDARGRYTIRFYIVAILFVVFDVETIFLFPWAVQFKLLGLFGLLEMGMFLGIL
                     IVGYIWIWKKGALEWV"
     gene            complement(11830..13185)
                     /locus_tag="VFC15_18555"
     CDS             complement(11830..13185)
                     /locus_tag="VFC15_18555"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZM12217.1"
                     /translation="MAQNKYILSERDIPKSWYNVIGDLPVPLHPPLHPGTGQPIVPQD
                     LSAIFPMQLIEQEVSGAPSIPIPDPVLDVLRLWRPTPLYRAHRLEKALGTPARIFYKH
                     EGVSPSGSHKTNTSVAQAYYNKVAGIKRLVTETGAGQWGSSLAFACNVFGLECMVYMV
                     KCSYFQKPHRRTMMETWGAHCIPSPSDQTAAGRQMLAEDPECPGSLGIAISEAVEEAA
                     GRADTNYSLGSVLNHVLLHQTIIGQEAVEQMKMAEAFPDIVIGCCGGGSNFAGLAFPF
                     VPYKAAGKDIRLIGVEPAACPTMTKGIHAYDFGDKSGMTPLLKMDTLGHDFIPEGIHA
                     GGLRYHGMAPLVSLLHREGIVEAVSYTQLKCFDAAITFAKTEGVIVAPETSHAVCSAI
                     DEALKAKEEGKEKTILFNLSGHGYFDMAAYERYFADELVDYEYPEEKIREALAHLPTV
                     S"
BASE COUNT         3098 a         4211 c         3749 g         2688 t
ORIGIN      
        1 acctttcacc gggtactctt tgccatcgag cttggcttcc cagttctcgc cggtcggggt
       61 agacatgctc agcccatcgc ccgtgccttt ccatgtagtg gtgagccctg cggcgtcttc
      121 attcacgttc tggatacgcc aggaaccgga gattgcactc gaacccgctg gccccttgga
      181 aacccgcgcg tacttggctt ccgccttgaa aggttggcta ccgtcggcgg gatagtttgt
      241 gctcgaaacg gtcagcatct tcccatccga tgacagggtc ctggtctgct cgaacgttgg
      301 cttgccggct ttcttgccgg tggcgtgaag cgtattcgcg tccaccacct gcaccgcgag
      361 cgtgtcatac ggctggccag tgacaggctg gtcctgcccg tcagccttta cgttgatttt
      421 ggggacgcaa ctttcgcagt cgtacatccc cttgtcgatg gaaaacacgt agggctttgg
      481 cgacagcttg gactcggcca tgttggtctt ccaggtaccg tcaaatggac tttgggcaaa
      541 caacattaca gggaaaagcc acaacacaac gcatagcaat ttcttcatgc tgatctcctg
      601 atccgaaatt ttctttcttg gttcccgacc gccccctcgc tacgtgcgcc gcaagggact
      661 caagttaggc gggtgttcta aggaaggtgg cattcagatc agactgattt agtccagcga
      721 tctgtaagac gtgagacatt tctcacacgc tggaaggtta tgttctacct cccagtttgt
      781 caaaccgtgg gcaccagtca ccgagccggc tacggtaggt ctaccgcatg agcatggcga
      841 catgcgatcc gtttaccagc cccagtgacc aggtcaccgc attgatgaag aagttcggga
      901 agatcccaat gccaacggtt cctgcagctg tgacgagcaa cgccaatccc atcgcagggc
      961 cgatgctaac tggctccgcg tccactgcgg gcttgatgat catggcactg gcgatgcgca
     1021 tgtagtagta catgctgatc accgcataaa tcacggcgac gctcgccagg atgtaatgcc
     1081 cggtctggat caggctgagg aagatgaagt acttgcccca gaagccagcc agtggaggaa
     1141 tgcccgccag cgataacagg aacagcagca tcagcatggt ttccgccggg gccttggaat
     1201 acagtccggc gatgtcgtcg atgtcgtcgc cgatgatgtt acgccgccgc agcgaggtga
     1261 tcacggcgaa cgcgcccaga ttcatgaacg tgtacaccag caggtaaatc atgatcgcca
     1321 tgatgccggt ggagttgttt acgccgtcgc tggccacgag tcccagcagc atgtagccga
     1381 cgtgcgcgat cgacgaatag gccagcagcc gcttcaggtt gttctgcgta attgccgcca
     1441 ggtttccgac cgtcatggtc acgatggcga cgaacaccag cagcggaacg tacatcgaac
     1501 gcagcggtcc caagccgaaa aggaagatcc ggagcaacag cgcccagccc gcggccttga
     1561 ctgccacgga catgaagccg gtgacgctgg ttggcgcgcc ttcgtaggcg tctggcgccc
     1621 actgatggaa cggcacagcg gcaatcttga acagcacgcc aacggtcgtg gtcagcaggg
     1681 cgaaggcgac aatgggatcg tgcgggcggg tcgccatcac ttcctgcaac ttctgcgcga
     1741 tgactcccag gttggtgctg cccgtaacac cgtagaacag tgacaggcca taggcgaaaa
     1801 tgccggacga aaatgcgccc agcagcaggt acttcaacgc ggcttcattg gagcgcttat
     1861 cgcgtcgcag gaatccaacc agcacgtagg tcgaaatggc catcaactcc aggccaatga
     1921 acagcaggac aatgtcgaag cccgcggcca tgcacatcat gccgaccacc gagaagagga
     1981 ttagcgcata gaattcaccg tggttttcgt gctcgacttc gaggtagtgg atcgacatca
     2041 atatcgcaat cgccgcgccc accagaaaaa ggtagaagaa gtagatggcg aaacgatcga
     2101 ctaacagcgt ccccatgaag ccggtgaacc ctgggacatc gtgaccacgc agctccgcca
     2161 ctcggtaggc tgaatgcaac tttcccaccg ctgctgccga gaacgctacg ccgattaacg
     2221 caatgtaggc gtttaccggc ttccattcct tcggcaagat caggtcgatc agcaggatgc
     2281 ccagcgcaaa cagcgcaagc agcaacatgg gcagcgccag ggcatagtcg gcattcgaaa
     2341 aatattgacc gatcgcgagg ttcacagttt catccctact tcccgctcgc cgggacgaag
     2401 gcatttggat tcgcgctcgc caccttcacc ggcacctgcg atttcttttg cgtcaatccc
     2461 gtggctgttg gactcgttgc cgtcggagtc ttcacctcag gagccttcgg cgcttccggc
     2521 aacggagtcg cggcatcggg ttgcgcggca acaaccggct tggttaaccc gggataatcc
     2581 ggacgaaccg tggcaaccag gttgttcacc ggagtcgcca atatctggaa cagcggcttg
     2641 ggatacagtc caatcgcaaa ggccagaatc acaagcggaa cgaaggttgc cagttcacgt
     2701 gccgacaggt ccttcaggct tgcgttcttc ggattgttgt tgggcccgaa gaacacgcgc
     2761 tggaacaacc acagcaggta cgccgctgcc agaatcactc ccggagccgc ccatgctgcc
     2821 caccacttat tctccatgaa cgtgccctga agaatcgtga actcgccgac gaacccgttc
     2881 agcaggggaa gccccatcga cgacatgaac atgatcagcg cgatggttgc gtacaccggc
     2941 atgatcgtgg agatgccgcc gtactcgctg atctcgcggg tgtggcggcg ttcgtacaag
     3001 attcctacga ttaagaagag tgcaccggtg gagatgccgt gattaatctg ctgaattact
     3061 gacccgctga ttcctagcgg actgcaggcg aagataccga gcgtacagaa tcccaggtga
     3121 ctcaccgagg agtaggccac cagcttcttc atgtctttct gcaccagcga aaccagcgcg
     3181 ccatagatga tgccgataat cgagagcgtc accatcaatg tgcgcaccca gggcatcatt
     3241 acgatccccg ggaagaacgg cagcgagaaa cgcaggaatc catacgttcc catcttcaac
     3301 aggacgccgg ccaggatcac cgagcccgcc gtaggcgctt cgacgtgcgc gtccggcagc
     3361 caggtgtgga acgggaacat cggcaccttg atcgcgaatc cgaggaagaa cgcaaagaac
     3421 agccacagtg ccgctggacc cgcgatcttt ggagccgtgg tgtacagctc aggaatcgag
     3481 aacgtgtaaa tgccagtgag cgtgtggtga tggaagtaca agaagatgat gcccagcagc
     3541 atcagcaccg aaccgaacag cgtgtacaag aagaacttga tcgcggcgta gagcttgcgc
     3601 ggcccgcccc agatgccgat cagcaggtac atcggcacca gcatggcttc ccagaacacg
     3661 aagaacagga agaaatcatt ggacataaag acgccgagca tgccggtctg cagcaacagg
     3721 aaccatgcgt aatactcttt agcgcggtcc tggatagctt cccatgacga caggatcgag
     3781 atcgcgccca gcagggtcgt gagcatgatc aggagaaacg aaatgccatc aatgccgatc
     3841 acatagcccg caccgatcga gggaatccag ttgttgggcg agccttccac gaactggaag
     3901 cccgcgcgtt ctttcaccgc ccagaaccac ggcaccaagg gaagcgaaac cgcgaatccc
     3961 aggaatccaa agatgttcgc gatccagcga atggcgtctt tgttttcctt cggcaccagc
     4021 agcaggagca cggcccccac cagcggcgtg aacagcacaa tcgaaagtat gtggttatac
     4081 attgcttagt tccaatccct gtggcggcgc agcccttccg gccgcatgaa ttccgcctct
     4141 accacttgac cacgtaataa aaaatgaagc acaccaggcc ggcgaccatg accagcgcgt
     4201 accactgcac caatccccac tgaatcagcc gcaccggata cgaagccaac cgcgtcagca
     4261 cggccactcc atttacaaag aggccgtcga tgatccactt atcccaccag gtcgaaatcg
     4321 ttccgctcat gcgagtggac cagccagcgc cgttcaccgc gccatcaatc acgttggcat
     4381 cgaaagtcca tagaccttca cccaagccca acgctcccag gcgcgccggc cccagcttct
     4441 tgcggccggt gaagacccag tcgtaaattt cgtccacgta atattttcgg aacaacgtct
     4501 tgtaaaagcg cggcgaaatt tcttggatcg gctcctgata tcccttctcg gcgtgcttgt
     4561 aagcccgctg cgccagccac caaccgacaa tcgcagcacc cacggaggcg aacatcagcg
     4621 catattccag tgggtcggta tgctcgacct gcttgtctcc tgcgaacact tgctcggatg
     4681 ccccttcgtt cacaaacacc cgggcatctt tggcgaacac cggctccagg aacttctcga
     4741 acttattcga tcctccgagg ctatgcggca tgcccagata tcccgcaccc accgagcaca
     4801 gcgcaagaat gaccagaggg atcgtcatcg tcttgggcga ttcgtgaatg tggtgctcga
     4861 cgtcgtgact catgcgcggc ttaccgaaaa atgtcaggta gatcagccgg aacatgtaga
     4921 acgaggtcat cagcgccgtc aggaacgcga tgtaccacag gtagcgatac gcgcccgctt
     4981 cgcgcgacca cgtctcccac aaaatcgcgt ccttggagaa gaagcccgcg aagccgggga
     5041 tacccgcgat tgccagcgtg ccgatcagca tcgtcccaaa ggtcgtctta atctttccgt
     5101 gcagattgcc catgttctgc atgtcctgct cgccactcat ggcatgaatc accgaacccg
     5161 agccgaggaa cagtagcgcc ttgaagaacg catgtgtgaa cacgtgaaac acgccagcgc
     5221 cgaacgcgcc gacaccaagt gccagaaaca tgtatcccag ttgcgagact gtcgagtagg
     5281 ccagcacgcg cttgatgtcg ttctgcacca gtccaatcgt cgccgcgaag atcgctgtca
     5341 gcgctcccac gatcgccact gtcttcatgg catgcggagc cagcacgaac agcgcattcg
     5401 atcgcgccac catgtacacg cccgccgtca ccatggtcgc ggcgtggatc agtgcagaaa
     5461 ccggcgtcgg accttccatc gcatccggca gccagacata caacgggaac tgcgccgact
     5521 taccgcacgc gcccacgaac agcaacaagc aggcagcggt gatcaccgga tcgccgattg
     5581 aaaatgcgcc gctacgcgcc attgcattga cttgcgtgta ttgcactgac ccgaaatgcc
     5641 aggcgatcat gaacatgccc aacaagaatc ccgcgtcacc gatgcggttc acgatgaatg
     5701 ccttgttcgc cgcagtcgag gctgagtggc gcaggaagta gaagccgatc agcaggtacg
     5761 aacacaggcc cacgccctcc catcccacaa acatcagggc gtaattgttg gccaagatca
     5821 aggtcagcat cgagaacatg aacaggttca ggtatccgaa gaagcggtag tatccgccct
     5881 cgtgcgccat gtaacccacc gagtagatgt ggatcagcat gcccacgccg gtcacgaaca
     5941 gcagccagat gctcgacaac ggatcgagca ggaatcccgc gtcggccttg aactgggccg
     6001 tatgtccgcc gttagtaacg tagctcagcg aactgtcgcc ggtgcccagc caggtgaaca
     6061 tcaccttctc gaacggcttg ccgggattcg cggccgagta cctggtgtac tgccaaaccg
     6121 ccgcacacgc ccacaggaac gccagcgcga ccaggccgac gcaaatgccg ttgaccgccg
     6181 acttcgacag cttgcgtccg aagaagaaca tgcaagccgc acccacggcg ggaaacagcg
     6241 gaatcaacca gatgtggtct aaaaagtaca tttttaactc gtagccttca gcaatcagca
     6301 ataagccgca agcagttagc tcaaagcaat atcgcaactt cgcccgaagc ccctcttctt
     6361 gcgcttctgg cttctggctt ctggcttatt gcttattgct tgttgcttgt tgctctccta
     6421 ccatctcaac aattctgctt cgtccacgtt caccgtctct ttgttgcgga agaaggcgat
     6481 gatgatgccc aatccgactg cggcttccgc cgcggcatcg gtgattacaa atatcgaaaa
     6541 gatttgccca tgcagcccgt acaaccgcga aaatgccacc aggttcaggt tcacggcgtt
     6601 cagaatcaat tcaatcgaca tcaggatgat gaccacgttg cggcgggtca gcacaccaat
     6661 cgtgccgata atcaacagcg ccgccgcgac taccaggtaa tgcaatgttg tgatctcgcc
     6721 catgtcagat cctcttcttc gccatgacca ccgcgcccac gatggctacc agcagcaaca
     6781 gcgatgcaat ctcaaacggc agcatatatt gaccgtagag cgccagcccg atctgctgtg
     6841 tgttttgtcc ttcgggcaac gttgcgctga gggtgatggg aaatatcgat cttccctggc
     6901 gaatcacgaa caggagcaac ccgcccagcg ataacgccgc gatcagacct accatccact
     6961 gcttgttgaa ctgaatttcg cggatgttct ggtcgaggtt caccagcatg atcacgaaca
     7021 ggaacagcac catgattccg ccggcataga gcacgatctg cacgccagcg acaaacggcg
     7081 catagagcat gaggtagagc ccagccagcg agaccagcgt cacgatcagc gacagtgccg
     7141 catgaacggc gttcttgcgg gtaatcaccg caatcgccga agcaactgcg atgatcgaca
     7201 gaaagtaaaa gaagaatgta gttgcgactg ggttcatatt ctcgtctgtc gtcggcttaa
     7261 gccggctcgg atccttgtaa atgcccttag cagcacggcg aaaggcgtgc cctttcaaaa
     7321 cttcgtatcc ctgacgccga cctgaaggtc ggcattaccc tgcgttcaac gatcttttca
     7381 actaatcact agccactaac cactagccac tacttctcgt acctcaccgg ctcgggtccg
     7441 cgctccagtg ccgcctggtc cagcaccatg ccgtcgcggc tgtagagcgc catctcgtag
     7501 tcttgcgtca attcgagcgc atccgtcgag cacgcctcgg cgcacaggcc acagaacata
     7561 cagcggctca ggtcgtaggt ccacgagacg acttccttgc gcttggtcac cgagttgcgc
     7621 tcgcccttga ccgtaatcaa ctgctccgga cacgccagcg cgcacaggtt gcagactatg
     7681 cacaacgact cgccagtgtc gggattagta ttgagccgtg gctgcccgcg atagcgttca
     7741 aagatcaccg ggcgctccag cggatactgc tcggtgatcc gttccttttc cgattggttt
     7801 tggaacgtca ccgacatgcc cttcagcagg tcaacgagaa aaatcttccg gagcaacgga
     7861 gccacactca ctgcaatacc cccacgatgg ccgcccacaa ttcctggcgc agaccgacga
     7921 ccgcagttgc gatcagcacg accaggccga ttggcagcaa caccttccag cccactttca
     7981 tgagctggtc aaagcggtag cgcgggaacg tgccgcgata ccagatatat aagtacataa
     8041 agcttgaaac tttgaagacg aaccagaaga tgtcctgcac ccgctcgcgt acgccgggca
     8101 ccagcaggaa caatccgagc agcaccagca aggcaccgaa cacagccaag ccaatcgtct
     8161 gggcacgcat ctgcgcaatc ttgggcatgc ggacagttcc caggaagcat cccgcgccca
     8221 tggctagaaa cgacagcgcc ggaaccagcg agaacgccaa gtcccatgct ggtccgtgca
     8281 ataggtttgc gaacggccgc agccatccgc ccagccacag cgtcacagcg atggacgaca
     8341 ccgcaagcat ggccgtgtac tccgccagga aaaatagcga ccagcggaac ccgctgtact
     8401 cggtatgaaa tcccgccacc aactccgatt cagcttcggg cagatcgaaa ggcgcgcgat
     8461 ttgtttccgc gatcatggcg atggcaaaca cgatgaatgc aataaatccc agcggaaaga
     8521 acttgaagat gaaccacatt ccctgggcat tttgcgcctc gacgattccg atcatgctca
     8581 gcgtgccggt gccggtttgc aggctggtca tcaacaccgc gcacacgatg gccaggccca
     8641 tggcgatctc gtacgacacc atctgcgcgc tcgaacgcag cgagcccatc agcggatagt
     8701 gcgagttcga cgcccatccc gccatgatga tgcccagcac gcccagcgac gaaatgccca
     8761 gcataaacag cacgccgatg ttcatgtcgg ttacggcatg agtgcgtcca aacggaacca
     8821 ccaggaacgt cgtgaacgcc gtcatggtca ccacgaccgg cgcgctccag aacaccactt
     8881 tgtcggcatt cgtcggaact atatcttcct tgatgagcag cttcagcgta tcggcgaagg
     8941 gctgcagcag cccgtgcggg cctacgcgca ttggaccgag gcgaatctgc atgtgcgcca
     9001 ggactttgcg ttccagccag gtcatggcga tcaccgacag cgaaagaccc agcacgatgg
     9061 cgatgatgta caccgtggcc cacagttggc ttcccgcctc gcccggcgtg accgctgaac
     9121 tcaaataatt cttaatggat tcgatcatta gcgatccacc tcgccgagca cgatgtcgag
     9181 cgtgccaatg accgcaacca catcggccac cagcaagccg cgcaccatct tgtcgagagc
     9241 ttgcagattt acgaacgatg gaggtcgcac gcgcacacga tacggctgcg tggagccgtc
     9301 actgactatg aagtagccca actcgccctt gggcgcttcg atcgagtgat acacctcgcc
     9361 caccggaggc ttgattacct tcggcacctt cgccatgatg gggccggcag gaatcgcttc
     9421 aatcgcctgt tgcagaatgc gcactgactg ccgcatctcc gcgacccgca ccaggtaccg
     9481 gtcataagta tcgccgttca cgccggtcgg aatctcgaag tcgaagtcct tgtacgactc
     9541 atacggctgc gccttgcgca ggtcccactt gacgccgctg gcgcgcagca ccgggccagt
     9601 tacgcccagc gcgatgcagt cttccggatt gagaattccc acacctttag tgcgcgtcag
     9661 ccagatgcgg ttggtggtca gcagttcttc gtactcgtcc agcttcggaa tgaagaactc
     9721 gcagaacttc tttacgtcct gctcaaagcc gtcgtaggtc tcgtaaatgc agccgccgat
     9781 gcgaaacgcg tgcgtggtca gccgggcgcc gcaatacttc tcgaagatct tcagaatctc
     9841 ttcgcggtcg cggaaggcgt aaaacagcgg cgtcagcgcg ccaatatcca gcgcatgtgt
     9901 gcccagccaa acctggtggc tggagatgcg gcaaagctcg gtgagaatca cgcgaatgta
     9961 ctgcgcgcgc ggcggcgctt ctacgctcaa caatttttct actgtctcgc agtagcccag
    10021 cccgttcgaa accgccgcca cgtagtccat gcgatccacg tacggattga acatggtgta
    10081 ggtgcggttc tcgccgatct tctcgactcc ccgatgcagg tagccgatga tgcactcggt
    10141 gcccaaaact ttttcgccat cgagcttcag gatgacgcgc aacactccat gcgtggacgg
    10201 gtgctgcggc cccatgttga gcaccatttc ggtggagtcg aggaaggttt cgtccttggt
    10261 gtcgagatcg aggtaggagg tgtcgatcgg ctcgctcatt gtccactctc gattccgagg
    10321 ttggcctgca cccactcgcg gtcttgcagc agtatgtcgt aatccttgcg cagcggatgt
    10381 cccttccatt cgtcgggcaa cagaatgcgc ttcagtccag gatgcccgtc aaacacaatg
    10441 ccaaacatgt cgaagacttc ccgctccagc cagttggctg tctcccagat cgataccgcc
    10501 gaggggacgt gttcgccgtc tttgatcctg gtcttcaccc gagtccgctc gttcttggga
    10561 aacgagtaca gcacgtacac catgtcgaat tgctgctcgc gcttggggta gtgcaccgcc
    10621 gtgatgtcga cgcagtagtc gaagccttcg tcatcgcgca tccgccgcag aatttccgac
    10681 aggatcgact gatcgaccac catgtaggtt tgccccaggt atttcgaggc ttcttgaatc
    10741 gctgacccgt attgttggcg cagttgcgcg gcaaatttcg actcccacgg ctcgggaacc
    10801 ggacctgcag gctttgccgg agcggcaggc ggcttgggcg cagccgcggc tgcgggaacc
    10861 gcaggcttag ctgctgcagc gggtttggcg gattcgactg gatttgcttc tgttgcgggc
    10921 gttaccttcg ccgattcggc gccgactgcg ggagccgctg gctttccggc ggcgtcagcg
    10981 ttcgaaggcg ctttagcgcc ctctggctgg ggcaaaacgg aatccggttc cttggtctct
    11041 tcgggcattc aatgtctctc tgttagctgc tggttcacta gtcaggtgga accttataag
    11101 ttgtagcagc ttcaaccact aactatgacg tgcgagggtc acgacagact gtgactccaa
    11161 tcacaacggt agaaaaatca tttttctttt gcttggcaat ccgaccactt tcccgaccaa
    11221 cggtcaagac cgtgggcgca aacaaaaccc atctgcaaca attgattgcg gatgggtttt
    11281 gctgaaactt ggcagcgcga gaggaacttc tacacccact ccaaggctcc ctttttccag
    11341 atccagatat atcccacgat caggatgccc aggaacatgc ccatctccag caatccaaac
    11401 agccccaaca acttgaactg gactgcccat ggaaacagga aaatcgtctc tacatcgaac
    11461 accacgaaca gaatggcgac gatataaaac cggatcgtgt agcgcccccg cgcgtcgtca
    11521 aacggcgtta tgccacattc gtagggcatc agtttgatgg tgttgggttt tctgggccgg
    11581 atcagcgtga acagcgccaa cgtccctgcg gctacgagaa acgcaaccaa agcaaagata
    11641 aaaatcggta cgtaattctg tggcatggtg aattgccctt attgtcactc cacgcgggag
    11701 cgtcattaac aagaagcgcc actataccgc ctcccgagcg acgattcaat gacacacgtc
    11761 acaaattggc catcgacgag tagggtctcc gggcatccgg atgtttgcca atccaccgac
    11821 cgctggccct caagacacgg tcggcaaatg cgcgagcgcc tcgcgaattt tctcttccgg
    11881 atactcgtag tcaaccagct cgtcagcaaa atacctttcg taggccgcca tgtcgaaata
    11941 accgtgaccg ctgaggttaa agaggattgt cttttccttg ccctcttcct tcgccttgag
    12001 ggcctcgtcg atggcgctgc agaccgcatg cgaagtctcg ggcgcaacaa tgactccttc
    12061 ggtcttggcg aaggtgatcg cggcgtcgaa acacttcagt tgggtatagc tgactgcctc
    12121 cacgatgccc tcacggtgaa gcaggctcac cagcggcgcc atgccatgat agcgaagccc
    12181 cccagcgtga attccttccg ggatgaaatc gtggccgagc gtgtccatct tgagcagcgg
    12241 tgtcatgccg ctcttgtcgc cgaaatcgta ggcgtgaatg cccttggtca tagtcggaca
    12301 cgccgccggc tcgacgccga tcaggcggat gtccttaccc gcagctttgt agggtacaaa
    12361 cgggaatgcc agacccgcaa aattactgcc gcccccgcag catccaatca cgatgtcggg
    12421 aaacgcttcc gccatcttca tctgctcgac ggcttcctgt ccgatgatgg tctggtgcag
    12481 cagaacgtga ttcaacacgc tgccgagcga gtaattcgtg tccgcgcgac ccgcggcttc
    12541 ctccaccgcc tcgctgatgg cgatccccag gcttccggga cattccgggt cttcagccag
    12601 catctgccgt ccggccgcag tctgatcgct gggagaaggg atgcaatgcg ctccccaggt
    12661 ctccatcatg gtgcggcgat gcggcttctg gaagtagctg cacttcacca tgtacaccat
    12721 gcactcgagg ccgaacacat tgcaggcgaa agccagcgaa ctgccccact gccccgcgcc
    12781 agtctcggtc accagtcgct tgatgcccgc gactttgttg tagtatgcct gcgccactga
    12841 ggtgtttgtc ttgtggctgc ccgacggcga aaccccttcg tgcttgtaga aaatgcgcgc
    12901 gggtgtgccg agtgccttct ccagccggtg cgcccgatag agcggggtcg gccgccataa
    12961 gcgcagcacg tcgagaacgg gatccggtat cgggatgctg ggcgcgccag atacttcctg
    13021 ctcgatcaac tgcatcggga atatcgcgct caggtcctgc ggcacaatcg gttggccggt
    13081 gccgggatgc aaaggagggt gcaaagggac aggcaggtcg cctattacgt tgtaccagga
    13141 cttcggaata tcgcgttcac tgaggatgta tttgttctgc gccataagcc cctctcggat
    13201 cgtacggatc gataccggtc gccaggatgt ggggtttgaa gatcgaagaa tagcacagcc
    13261 caaaccctcg ttaagctccc aaagacaacc ccgaggttaa aaccaaatcg cagctccgcg
    13321 gtttcacctt ccgacccccg cgtccggtca caacggagaa gcaaaactga gccatggggc
    13381 tcggagcagc accaaggtat ccggcgcggc ggagccgcga ttcgctggta ggcgtgggcg
    13441 taagcccgcg ctccaccagc ccgatggaag tcccagccgc ggagcggcgt ttttcgcccg
    13501 ccccgcgcct tctgcgaaag gggaacatca atcccatcgc gatccgacgc ggcggagccg
    13561 cgatgcgctg ttaggcctgg gcgtaagccc gcgcaaaacg gaccagacgg aatgcgagcc
    13621 gcggagcggc gtccttcggc cggtggccta catatcccta gttttgggat atgtggggct
    13681 tttgccttct gctgcgcgac tcacatacaa ctcaccacac tctagtggca cggtacagtc
    13741 tagccc
//
LOCUS       DASRKK010000772         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1158898, whole genome shotgun sequence.
ACCESSION   DASRKK010000772 DASRKK010000000
VERSION     DASRKK010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158898"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFC15_18560"
     CDS             <1..433
                     /locus_tag="VFC15_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12218.1"
                     /translation="LPWATTSCELKRRPSRLWLWRKLRCVRASRLVNKRPIYVTVVAY
                     ILIAAGAIGIAYHFTEFRVAPPTEYFGILVVRLIAVIAGVYVLRGKDWARWLSIAWIA
                     FHLAISIFHTKEGVAVHAVVLITFTVLLFLPASNRYFRSRV"
     gene            619..3675
                     /locus_tag="VFC15_18565"
     CDS             619..3675
                     /locus_tag="VFC15_18565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZM12219.1"
                     /translation="MSWQPREFKLTTQQKSAVDHVDGPILAVAGAGTGKTTVLACRVV
                     RMIEDKLASPREILAVTYTRNSARDLLKRIARLWKGADDPATVAQVAKTGLKVGTFHS
                     YCYSLLNLAGKRFELIEDTDLYVLLRRRIADLKLQYYIKAATPGEFLEGLTGFFKRCH
                     DELRSPDDYDAYVAKLKSKQLPLPRVGHSKQAESMSDDEIFGRCHEIARVFRYAEDLL
                     ASENLGTYSHVITRAIALLRDQNNAVHLDRARSGARFVLIDEFQDSNVAQIELTRLLV
                     ADQANVFAVGDPDQAIYRFRGATAGAFDHFLKTFGAGRVKRVTMSENRRSTDVILKSA
                     DSIISRNPEITSVELPGGERWKRAPLKHARTKPETVPVSPVQVKAWEQAEGEAAFVAE
                     EIIRKHQSEGRPWRDFAVLYRSHSNRDALIEQLLLLDVPFTVEGIDLLEIAAVRDLLA
                     ALRAIESGDAVGLLRVAALPRFHVEGDAVRAALAAAGEDVSLEAVLDKVPGGSEVITA
                     LAEARHDTQRFQSKALTACGLAQKHFGIAPSSETEGFIQFVESWSRKPKQISGEGTLR
                     EFLEYLDYFIEANGKIVDPEADAEGTPATLQMEIGIPAKAERTDDAVRLLTVHAAKGL
                     EFPVVFVLRVCSPSFPSHYREDLVEFPSELHNKDTMPEGSPKELHREEERRLFYVAVT
                     RAEDQLFLLAKKGTGKKDPTPPGYLRELLTAGAKSTKDSVEFATIPSGEVVRTIHAGA
                     QPMPRITEWVNLPPLPQTISRSLSASAVERYERCPLSYKLNIEWNLPEEPGANMQFGA
                     AMHLALLAYFDSVRKGRSMSVDEVVDYFCSEFQKAKIDDPVQRELYERDGCAQLKSFL
                     ASPAATPRGAVALVEYWFKCEIAGTRVTGRIDRVDEDADGYVVVDYKTGNPKSQKTAD
                     DSLQLSVYAIALGATKPVKALIFQNMQDNSTVETTRTPEDLLKTEAKIVEVAAGIAAG
                     QFDASPGRHCNWCTYRTICPEKEVIMPSSAVKRSGPTDWGADCAAG"
BASE COUNT          836 a         1177 c         1159 g          864 t
ORIGIN      
        1 gcttccctgg gcgacaacat cctgcgagct gaaacggcgg ccatcgcggc tctggctgtg
       61 gcgcaagctg agatgtgtta gagcctctag acttgtgaat aagcgtccta tctacgtaac
      121 agtcgttgct tacatcctga ttgcggcagg ggcgattggg atcgcctacc acttcaccga
      181 gttcagggtc gcgcccccga cggagtattt cgggattctg gttgtacggc tcatcgcagt
      241 tatagcgggt gtgtatgtgc tccggggcaa ggactgggca cgctggctgt caatcgcttg
      301 gattgccttt cacctggcga tcagcatttt tcacaccaaa gagggcgtgg ccgtacatgc
      361 ggtcgtgctc ataacgttta cggttttgct ctttctacct gcgtcgaacc gatacttccg
      421 ctcccgcgta tgagagccaa tcactttttt tcgcctaaga ctacacgcga aattacggct
      481 gcacgttttg atccactttt tcctcaactt cggcgagctt tgcacatgaa tctcggccgt
      541 taggcttgtc tcccgtcaca ctgcggcgga taatagagtt tcgctttttc ttcgataccg
      601 gatcgagagc acaaacccat gtcctggcag ccccgcgaat tcaaactcac gacgcagcag
      661 aagagtgccg tcgaccacgt cgatggaccg attctcgcgg tagctggtgc gggcaccggc
      721 aagaccacgg tgctggcctg ccgtgtcgtg cgcatgatcg aagacaaact cgccagtcct
      781 agagaaattc tcgctgtcac ctacactcgc aattccgcgc gcgacttgct caaacgcatc
      841 gcgcgcctct ggaagggcgc ggacgacccc gcaaccgtcg cacaagttgc gaagactggt
      901 ctcaaggtcg gtacattcca ctcgtactgc tactcgctgc tcaacctggc cggtaaacgt
      961 tttgagttga ttgaggacac cgacctctac gtgctgttgc gacgtcgcat tgcggacctg
     1021 aagctgcagt actacatcaa ggccgccact cctggcgaat ttcttgaagg actgaccggc
     1081 tttttcaagc gttgccacga cgaactgcgc tcgccagatg actacgacgc ctacgttgcg
     1141 aaactgaaaa gcaaacagct tccactgcca cgcgtgggcc attccaaaca agccgagtca
     1201 atgtcagacg acgaaatttt cggccggtgc catgagattg cgcgcgtctt tcgctatgct
     1261 gaagatctgc tggcgtccga gaacctcggc acctacagcc acgtcatcac tcgcgccatc
     1321 gcgttgctgc gcgaccagaa caatgctgtc catctcgatc gtgctcgcag tggcgcgcgc
     1381 ttcgtgctga ttgacgagtt tcaggattcc aacgtcgcgc aaatcgagct gacccgtttg
     1441 ctcgtcgccg accaggccaa tgtgtttgcc gtgggggacc ccgaccaggc tatctatcgt
     1501 ttccgtggag caactgcggg agccttcgac cactttctca agacctttgg cgccggccga
     1561 gtgaagcgcg tcaccatgag cgagaaccgc cgctcgacgg atgtgattct caagtcagcc
     1621 gacagcatca tttctcgcaa tcccgagatc accagtgtcg agttgccggg gggcgaacgc
     1681 tggaagcgag cgcccttgaa acatgcccgc accaagcctg agacggtccc tgtttctccc
     1741 gtgcaggtaa aggcctggga gcaggccgag ggcgaagcag catttgtggc cgaggaaatc
     1801 attcgcaaac accaatctga aggacggccg tggcgcgact tcgccgtgct ctatcgcagc
     1861 cactcgaatc gtgatgccct gatcgagcaa ctgctgctgc tggatgttcc attcaccgtc
     1921 gaaggcatcg acctgctgga gattgctgcg gtgcgcgacc tcttggcggc actgcgcgcc
     1981 atcgaaagtg gcgacgccgt gggactgttg cgtgtggccg ctcttcccag gtttcacgtc
     2041 gagggagacg cggtccgcgc cgcgctggca gcagcaggag aggatgtcag cctcgaagcc
     2101 gtgcttgaca aggtcccggg cggctccgaa gtcatcaccg cgctcgccga ggctcgtcac
     2161 gacacccaac gcttccagag caaagcgctg accgcatgcg gccttgctca aaagcatttc
     2221 ggcatagctc cttcgagtga aaccgagggc ttcatccaat tcgtcgagag ctggagccgg
     2281 aagccgaaac agatttcagg cgaaggcacg ttgcgcgagt ttcttgagta cctcgactac
     2341 ttcatcgagg cgaatggcaa gatcgtcgac cctgaggcag atgccgaagg caccccagcc
     2401 actttacaga tggaaattgg catccccgcg aaagccgagc gcaccgacga tgccgttcga
     2461 ctgctgaccg tacatgctgc caaagggctg gaattcccgg tggtattcgt gctgcgggtg
     2521 tgttcaccgt cgtttccctc ccactatcgg gaggacctgg tcgagtttcc ttcagagctt
     2581 cacaataaag acacgatgcc agagggatct cctaaggaac tgcatcgcga agaggagcgc
     2641 cggcttttct atgttgcggt cacgcgcgct gaggaccaac tcttcctttt ggctaagaag
     2701 ggaactggca agaaagaccc gacgccaccc ggctatttgc gcgagctctt gacggccggt
     2761 gcgaaatcga ccaaggacag cgtcgaattt gccacgatcc caagcggcga agtggtccgc
     2821 acgattcatg ccggcgcgca accgatgccg cggattacag agtgggtcaa tctgccaccg
     2881 ctgccgcaga ccatcagccg gtcgctgagc gcgagtgccg tcgaacgcta tgagcgctgc
     2941 ccgttgagtt acaagctgaa catagagtgg aaccttcccg aggagcccgg ggcgaatatg
     3001 cagtttggcg cagcgatgca tttggcgctg ctggcgtact tcgattccgt acgcaaggga
     3061 cgctccatgt ccgtcgatga ggtggtggac tacttttgca gcgagtttca aaaggcgaag
     3121 attgacgatc cggtacagcg cgagctgtat gagcgcgatg gatgcgcgca gttgaaatcg
     3181 tttctggcgt cacccgcagc aacaccccgc ggggcggttg ccctggtgga atactggttt
     3241 aagtgcgaga tcgccgggac ccgcgtcacc gggcgtatcg accgggtgga cgaggatgcc
     3301 gacggatatg ttgtcgtgga ctacaagacc ggtaatccca aatcgcagaa gaccgcggac
     3361 gacagtctgc agctttcggt ctatgcgatt gcgctcggag ccaccaagcc ggtgaaggcg
     3421 ctcatcttcc agaacatgca agacaacagc accgtcgaga cgacgcgcac accggaagat
     3481 ctgctcaaga cggaggcgaa gattgtcgaa gtcgcggcgg gtattgcggc gggccagttc
     3541 gacgcctctc cgggacgcca ctgcaattgg tgcacctacc gtacgatttg tcctgagaag
     3601 gaggtgatta tgccctcgtc ggctgtgaaa cggtcaggac caactgattg gggcgccgac
     3661 tgcgccgcgg gataaagctc cacgcaaaag gctaggggac gcttgcgcgt cccctggtgt
     3721 acggtctgaa gaaaagttac cgttttttct tctttgcagc cttcttcgcc ggtgcttttg
     3781 cctttttctt agttgccatg ttgtctattc tccctttcca tctgtgatgg atcattgcga
     3841 cgtaacatca tcgcaatttg ttctttgtat agagttatcg aaaaaaatag tcaagaaaaa
     3901 aacgcgcacc aagttgagta attcggtgac gcgtctctcg catcgcgacc ccgcatgagt
     3961 gcggtcctca ccgtctccgt tgccgcgatc ggctccgccg attgttcatg tgcgcgaaac
     4021 ctgcatgaac actggc
//
LOCUS       DASRKK010000773         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_865658, whole genome shotgun sequence.
ACCESSION   DASRKK010000773 DASRKK010000000
VERSION     DASRKK010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865658"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..711
                     /locus_tag="VFC15_18570"
     CDS             118..711
                     /locus_tag="VFC15_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM12220.1"
                     /translation="MVATAFSSLVPMETEESQIARGLRRRDPDLLDALIEQYQHRLLR
                     YLLHLTGNRAVAEDLFQETWLRVLEKGHQYDGRNRFVTWLMSIGHNVAIDYLRKKNPR
                     SLDELKDPEDGAPFEPAATGPSPFDLALAQQQHEMFDAALGQVPAIFREVLVLRFQEQ
                     MKLEEIAQLIHIPIATVKTRLYRGVMALRPALTGGAL"
     gene            708..1217
                     /locus_tag="VFC15_18575"
     CDS             708..1217
                     /locus_tag="VFC15_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12221.1"
                     /translation="MTNREHERAIDLITRHGVEDIAAPDTAWLDLHLAACAECSDYAD
                     LAESTGRLLRSVAVTASPALVATTQARLRARATYLHEQRARMVLIAISFCIGAMSSAV
                     SAWLWWRFGGWVAQHVGLSSAIVEPGILLFLLLPAVVIAGLLLAFPHPIFEGSLMLGL
                     AGEHEGEKR"
     gene            1214..1717
                     /locus_tag="VFC15_18580"
     CDS             1214..1717
                     /locus_tag="VFC15_18580"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZM12222.1"
                     /translation="MNQSKSHFRSELAIIPPIARVIAVLVFLLVQICLLELVPRYVHG
                     PDVPPMPALVLISLVGGAVLGIIVLMVGYVYADSKRRGMNALLWTLLVIFVPKALGFI
                     AYFLLRKPLLIPCPNCGTEVGSDFRYCPKCGHAVTPSCANCGRSISRDYVCCPYCGRP
                     VGATATS"
     gene            1923..>2102
                     /locus_tag="VFC15_18585"
     CDS             1923..>2102
                     /locus_tag="VFC15_18585"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZM12223.1"
                     /translation="MAPVTYLEAIRQGIWEEMERDPAVFCIGEDIGIYGGAFKVTEGL
                     IDRFGPERVVDTPISE"
BASE COUNT          412 a          615 c          618 g          457 t
ORIGIN      
        1 gcgacttaac cggaacctgg gcatagaccc caataaaatc cgtgtcatcc gtgcaaatcc
       61 gtggtgaaaa tgtcttcctg agtctttttc tccccctgcc tcgtatatcc cgttagtatg
      121 gtcgccaccg ccttcagttc gttggttccg atggaaaccg aagagtccca aattgcgcgt
      181 gggctgcggc gtcgcgatcc tgacctgctc gacgccttga tcgagcagta tcagcaccgg
      241 ctgctgcgct acctgctgca cctgaccggc aatcgcgccg tcgccgagga cttgttccag
      301 gaaacctggc tgcgcgtgct ggaaaaaggc catcagtatg acggccgcaa ccgcttcgtg
      361 acttggctga tgtccatcgg acacaacgtc gcgatcgact acctgcgcaa gaagaatccc
      421 cgcagcctgg acgaattgaa agacccggag gacggcgcgc cttttgaacc tgcggcgacc
      481 ggtccatcgc cgttcgacct ggcgttggca cagcagcagc atgaaatgtt cgacgcggcc
      541 ttaggacagg tgccggcaat tttccgcgag gtcttggtac ttcgcttcca ggaacagatg
      601 aagttggagg agatcgcgca gttaattcac attcccattg ccaccgtgaa gacacggctc
      661 taccgcgggg tgatggcgct gcgccccgcg ctgacaggag gcgcactatg acgaaccgcg
      721 aacatgaacg tgccattgac ctcatcaccc gtcacggcgt ggaagacatt gccgctccgg
      781 atacagcatg gttggatttg catctggcgg cgtgcgccga atgttccgat tacgctgact
      841 tggcggagag cacggggcgg cttctgcggt cggttgcggt cacggccagc ccggcgctgg
      901 tggccaccac ccaagctcgg ctgcgcgccc gcgcgaccta tttgcacgag cagcgagccc
      961 gcatggtgtt gattgcgatt tcattctgca tcggagccat gtcttcggcc gtttcagcgt
     1021 ggttgtggtg gcgattcggc ggctgggtag cgcaacatgt gggactctca tcggcgatcg
     1081 tcgagccggg aatcctgctt tttctgctgc tgccggctgt cgtgatcgcc ggtctgctac
     1141 tggcgtttcc acatcccata ttcgaaggct cactcatgct gggtttggcg ggggagcatg
     1201 aaggagaaaa gcgatgaacc agtcaaagtc tcactttcgt agcgaactcg caatcattcc
     1261 gcccatcgca agggtaatcg cagtattagt gttcttgctc gtgcagatct gcctgctgga
     1321 actcgtacct cgctatgtac acggtccaga cgtacctccg atgccggcgt tggtgctaat
     1381 cagcctggtt gggggcgcag tcctgggcat catcgtcctg atggtcggct acgtgtatgc
     1441 cgactccaag cgtcgcggca tgaatgcgct gctgtggacg ctgttggtga ttttcgtgcc
     1501 caaggccctg ggctttattg cgtacttcct gctgcgcaag cccctgctga taccgtgccc
     1561 caactgcggt accgaggttg gatcggactt ccgatattgc ccgaagtgcg gccacgcagt
     1621 gacgccgagc tgcgccaact gcggccgctc cataagccgc gactatgtgt gctgtccgta
     1681 ttgcggtaga cccgtgggag caacggcgac ctcctaggct caaacgaaac cgtgagcagc
     1741 gggctcacca cagaggcaca gagaaaaacg ttgttaccac ggacaacacg gagagcacgg
     1801 aaaagaaata acaaaagaaa ctccgtgatc tccgtgacct cagtggtgaa aaagaactcc
     1861 tttcttctct gtgcctctgt gtctctgtgg tgaattccgc gattgattta gaattccttc
     1921 ccatggcacc cgtcacctat cttgaagcca ttcgccaggg aatctgggaa gagatggaac
     1981 gcgatcccgc ggtcttctgc atcggcgaag acatcggcat ttacggcggc gcgttcaaag
     2041 ttaccgaggg gctgatcgac cgctttggcc ccgagcgcgt ggtggacacg ccgatctcgg
     2101 ag
//
LOCUS       DASRKK010000774        13172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_377971, whole genome shotgun sequence.
ACCESSION   DASRKK010000774 DASRKK010000000
VERSION     DASRKK010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377971"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(576..857)
                     /locus_tag="VFC15_18590"
     CDS             complement(576..857)
                     /locus_tag="VFC15_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZM12224.1"
                     /translation="MVTLNVNGVRREIQAPGDTPLLYVLRNDLQLIGPQFGCGLAQCG
                     ACSVLIDGKEVRSCITALSDVAPNQEITTLEGQPARWAKQGSCCRAEDG"
     gene            complement(1059..1286)
                     /locus_tag="VFC15_18595"
                     /pseudo
     CDS             complement(1059..1286)
                     /locus_tag="VFC15_18595"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1299..1454)
                     /locus_tag="VFC15_18600"
     CDS             complement(1299..1454)
                     /locus_tag="VFC15_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12225.1"
                     /translation="MQIKKSGSQPSGKGPAEYFTGTVRVDPLFDAPDPARVLGASVTF
                     EPGARTA"
     gene            complement(1492..1629)
                     /locus_tag="VFC15_18605"
     CDS             complement(1492..1629)
                     /locus_tag="VFC15_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12226.1"
                     /translation="MHLKVVAVTDATQVKSVVEKYRGKYGTSGVNLYAKLDVAVIAQT
                     R"
     gene            complement(1719..2561)
                     /locus_tag="VFC15_18610"
     CDS             complement(1719..2561)
                     /locus_tag="VFC15_18610"
                     /inference="COORDINATES: protein motif:HMM:NF005065.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM12227.1"
                     /translation="MSQRTWFITGVSSGFGRQLADQLLERGDRVVGTVRDTDKVADLL
                     ERYPEAFHAEVLDVTDTAAIRQVVERSFSRLGRIDVIISNAGYGLFGAAEELTDKQVE
                     HIVATNLVGSIQLIRAALPHLRAQGGGRVIQISSYGGQVAFPGNSMYHATKWGIEGFV
                     ESVAQEVASFGIGMTIIEPGGARTEFRYGSAQVAELMPIYDETPAHSFLRMLDPKNGL
                     APGDPARMAARIIESVNVEPAPLRMVLGSQALEGTLTTLRKRIADFETQKELAASTDF
                     PPGE"
     gene            3039..3356
                     /locus_tag="VFC15_18615"
     CDS             3039..3356
                     /locus_tag="VFC15_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12228.1"
                     /translation="MTTSQQALAPRSSTTLDTVHDILGTLVARYRNDEVLTCLRQIPA
                     REENFRPIPDWVSRELSDAYRAKGIRELYSHQAATAELVHGGRNVVVVTPTASRGEIV
                     QLS"
     gene            3587..4486
                     /locus_tag="VFC15_18620"
     CDS             3587..4486
                     /locus_tag="VFC15_18620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM12229.1"
                     /translation="MLTGVHILLTYQCTRECDHCFLHCGPQREGTFTLRQLRELLHEI
                     KKIHTIDTVYFEGGEPFLFYAVLLVGLRMVHDAGLRAGIVTNGYWANSLEDAEQWLRP
                     LLDLGIADFSVSDDEFHSTKGDQSPGALAYRAARKLGLPCAKICIAAPAVSLGHSTGS
                     EKGQPVTGGGVLFKGRAAEKLTAGLPVRPWQRLTTCPHEELANPERVHVDTFGNVHVC
                     QGVSIGNMLSTPLSELVRTYDAAKHPIVGPLVEGGPARLVERFGVEVADAYVDECHLC
                     YTARKLLLPAFPQYLAPAQVYGL"
     gene            4495..5124
                     /locus_tag="VFC15_18625"
     CDS             4495..5124
                     /locus_tag="VFC15_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HZM12230.1"
                     /translation="MRKTALLLALIVCIAVQAQDLKSIQLPAPQTDGSRPLMQVLKER
                     KTQREFGPEKLPTQVLANLLWAAFGINRPDGRRTAPSAMNWQEIDIYVALGDGLFLYN
                     AKANRLEPILAQDMRAATGTQAFVAAAPLNLVYVADLSKTGQSSSDAEFFTAADAGFI
                     SENVYLFCTSEGLATVVRGSVDRAALAKVMKLRPDQKILLAQSVGYPKK"
     gene            5142..5783
                     /locus_tag="VFC15_18630"
     CDS             5142..5783
                     /locus_tag="VFC15_18630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZM12231.1"
                     /translation="MVTEHRLGDIIANSVTHGVGAALAVAGAITLVVTAVGGTAWQIA
                     SCSIFGCSLVMVYVCSTLYHSLVRTRARHVLRIIDHSAIYLLIAGTYTPFVLVPLRGK
                     LGWLLFAIVWSLAILGIVFKSFAVDRFAIASVVVYLGMGWLVVFAARPLLQALTWHGI
                     FWIALGGLFYTAGIVFYALDRLSYFHAFWHLFVLAGSTCHYFAVLFYVAQPRS"
     gene            7142..7615
                     /locus_tag="VFC15_18635"
                     /pseudo
     CDS             7142..7615
                     /locus_tag="VFC15_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(7791..7935)
                     /locus_tag="VFC15_18640"
     tRNA            complement(7791..7935)
                     /locus_tag="VFC15_18640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7843..7845),aa:Leu,seq:taa)
     gene            complement(7989..9536)
                     /locus_tag="VFC15_18645"
     CDS             complement(7989..9536)
                     /locus_tag="VFC15_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZM12232.1"
                     /translation="MAPQHHFSGPITIFHERTLPERATPAGYSALIDAYGLRVPLPRT
                     LSATGEHHRTRNEAGWRILTPRHAPSPNLEGHLTFALKYEGLDLAVLKRLFAALAPAE
                     IETIVRAKPTGSYSRRIWFLYEWLIGRRLDLPDADKGTYEPVVGTEQQWAVPGENSPR
                     HRVRNNLPGTPEFCPMVFRTKSLEEFAAMDLVHRAQEVVANVPKDVLARTAAFLLLKD
                     SRASYAIEGERPPQDRIQRWGRAIGEAGKKSIELDELLRLQTIVIGDARFVKLGLREE
                     GGFVGEHDRETRLPIPDHISARPEDLRSLISGMGAFDRGPALNLDAVIAAAVLAFGFV
                     YIHPFVDGNGRIHRYLIHHVLAQRGFNPAGVMFPVSAAILARIDDYRATLEDYSGRLL
                     PMIDWEPTADSNVKVLNDTADFYRFFDATPHAEFLYACVKQTIEEDLPRETEFLERYD
                     GFRARIEAMVDMPERTIDLLFRFLHQNAGRLSKRAREGEFAQMADGEVAAAEAAYAAS
                     FMENNRQ"
     gene            10055..12829
                     /locus_tag="VFC15_18650"
     CDS             10055..12829
                     /locus_tag="VFC15_18650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM12233.1"
                     /translation="MTVTSRLFDAYLKCPTKCFLWSRGETGTSNAYADWARIQNISYR
                     SEGIRRLKDGVASNECVAEPLDGKNLKSAKWRLALDSKAYAENLESAIDALERVPRDT
                     PGKPPQFSPIRFVFRNKLNRHDKLLLAFDALVLSEALGRAVDFGNIIHGDAFVNLRVK
                     TSALGNEVRKITAKIATLLSGKAPDPVLNRHCAECEFRDRCKQKAVETDDLSLLAGIT
                     EDERIRYRSKGIFTVTQLSYTFRPRRTPKRAKNPGKPRYNALQALAIRENTVYIHGSP
                     QIPDSKAQVYLDIEGLPDSDSYYLIGVLILSEGQETFHSFWADQREQEPEIFARLANT
                     VCELPDFRVLHFGKYETAALERMKTRLPDSLHPKLDMIVARATNVLSVIHSHVYFPTY
                     SNGLKEIGRFLGFLRDDENATGLQSIIWRQKWQETKVPDCRARLLQYNQDDCRALKVV
                     CDFIRRLNSPEAATAAVAQTPFKTTHTEELTKDRPRWELFRPRKEYSTEDLKKVAECA
                     YFDYQREKVFVRTHPHFNVINKQHRKHNRTSFRINKHYSMESQRCPRCNSRNIQKGRQ
                     SSHELVDLKFFKGGVKKWITRTVSWKYSCSKCKHAFSSAEPRDRYKFGHGLLSWCVYS
                     NVACGLNMGRVERSLEDVFGLSFNQSQFFRSKRYIMALYRPLCEQILESILASPVIHI
                     DETTVRLQKQTGYVWVMTTLDKVYYFYKSSREGSFLQEMLAKFSGVLISDFYSGYESL
                     KCKQQKCLVHFVRDIDDDILKNPLDVELKSIAQEFGLLLRTIIETVDTRGLKTRYLRK
                     HKRSVVRFLDSVTSTDVLSPLANKYKKRFQKSGEKMFTFLDHDGVPWNNNNAEHAIKR
                     FARYRRDADGRFTERTLQEYLVLATVLETCEFNNLNVLKFLLSKEATLEGLLRMAGRK
                     TDLARAKRIP"
     gene            12863..13172
                     /locus_tag="VFC15_18655"
                     /pseudo
     CDS             12863..13172
                     /locus_tag="VFC15_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104584.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2865 a         3785 c         3716 g         2806 t
ORIGIN      
        1 gctgtgagga cacaatgcgg atgacgcctg gactccgcaa gacctcaacc ggttgggccg
       61 gggcagtctg cacgaatacg taccccgggg aaaagcggaa gtggaggttc cgaatcgtgc
      121 ggttccgccc agtgtctaag cagccgctga aacagatttt ggccgccaca cgcgatctgt
      181 ggaatcgaac cgacatcagc cccgccgtcc gcgagaactt tgaaaaaatg atcgactgcc
      241 gcacccctgc tttgggtcac ggaagtctat gactcagaaa ccgaggagaa actcgtttgc
      301 cacacctgca attagacatt caagaacggt caacgcagtc gtgagttctt gaccgccgca
      361 aactggagca cctccctcgg tcctggccaa gcccccgtga gcaaagctcc cgcctgcagg
      421 atccgctctc gaaatcgtcg gcagaactcg ccgcgagttc tacttctgct gaaaggacga
      481 cataacacta gcaatcctac agggcagatt tctttcaaga ttccacggcg gcttgcagaa
      541 tccttactac ttctcagcga acagcctctc gaaccttagc catcctctgc ccgacagcaa
      601 gacccctgct ttgcccacct cgcgggctga ccttcgagcg tggtgatttc ctggttagga
      661 gcgacgtcgg agagggccgt aatgcaggaa cgcacctcct tgccgtcgat cagtacggaa
      721 caggctccgc actgtgcgag cccgcagccg aattgcggac cgataagttg gagatcgttg
      781 cgcaaaacat aaagtagtgg agtgtcgccg ggtgcctgga tctcgcgccg gactccattc
      841 acattcagcg tgaccataga gaactccctt caaaggcaca ttcagaactc agatgactct
      901 tctacgtaaa aacccccctc tgtctcgcgg tcctcagtcc gcaagggcaa ggttcgcttt
      961 gccctgacgc ggatatctta actcgctgat acgctcgctc tgccaccgct tgcaagcggc
     1021 ggcgtttccg gaacttactc aatcaggcct aagctcaatc agctgtggta ctgttcgtcg
     1081 ctgaccttct ccatccagtc ggcgctttta ccttcgagct tttccacgat ggcgatgtgc
     1141 gtcatcgctg tcgtcggtgc agccccatgc cagtgtttct cgccgggcga gaaccagacc
     1201 acgtcgcccg gacgaatttc ctcgactgga ttgccccacc gttgcacgcg cccgcaccca
     1261 gccgtcacga tcagggtctg accgagcggg tgtgtatgtc atgccgtccg cgcgcctggc
     1321 tcaaaggtaa cgctagcgcc caatacgcgc gcgggatcgg gagcgtcaaa cagcggatcg
     1381 acgcgtactg taccggtaaa atactcggcc ggtcctttgc cggaaggttg tgaaccactt
     1441 ttcttaattt gcatcttgtg acttccttct ccattcgtga tggaatcacg atcatcgcgt
     1501 ctgggcgata acggcaacgt cgagcttcgc atagagattc acgccgctgg tcccatactt
     1561 gccgcggtat ttctcgacta cggactttac ctgcgtggca tccgtgacag caacaacctt
     1621 taagtgcact ctgttcacct ccagcatcaa tccgccgaac tataatttcc ggcttgcccg
     1681 tcatccggac acgatcggta tccggatgac agtatgggtc actccccagg cgggaagtcg
     1741 gtggaagcgg cgagttcctt ctgcgtttcg aaatcggcga tccgcttgcg tagtgttgtg
     1801 agagtgccct ccagcgcctg tgagccgagc accattcgca gcggtgcggg ctccacgttg
     1861 acgctctcga tgatgcgcgc ggccattcgg gccggatcac cgggcgcgag gccgttcttg
     1921 ggatcgagca ttcgcaggaa gctgtgtgcc ggggtttcgt cgtagatagg catgagctct
     1981 gcaacctgcg cgctgccgta gcggaactca gtgcgcgcgc caccaggctc tatgatcgtc
     2041 attccgatcc cgaaggatgc gacctcttgt gcaaccgact ccacgaagcc ctcgatgccc
     2101 cacttcgtgg cgtggtacat cgagttgccg gggaaggcga cctgcccgcc ataggaagag
     2161 atctggatga cacgtccgcc gccctgggcg cgaaggtgag gcagcgcggc acggataagt
     2221 tggatcgaac cgaccaggtt cgtcgcaaca atgtgttcga cctgcttgtc agtgagctcc
     2281 tccgccgcgc cgaacaggcc atatcccgca ttgctgatga tcacgtcaat gcgtccgagt
     2341 cgggagaacg agcgctcgac aacttggcgg atggctgctg tgtcggtcac gtcgagcact
     2401 tcagcatgga atgcctcggg ataacgctcg agcagatcgg caaccttgtc ggtatcgcgg
     2461 acggttccga cgacgcggtc gccgcgctcg aggagctgat cggcgagctg acgaccgaag
     2521 ccgctgctga cgccagtgat gaaccatgtg cgttgtgaca tcgtgaatcc tccggtaata
     2581 gttgcaatcg tccgcgtgag ccgacagctc cctgcccagt tacatctctc tgcacactgt
     2641 acccgtgtca agaggcgaca gatcagtgcg cgagcatcgc gtccaagtcg tcacgcaccc
     2701 gtttaggctc gtcctcaagc ggcatgttcg ccgcgtcacg tatgccgtcc agggagcctt
     2761 gcgggtccga cgccatcgat catgcccgca ccgtgatcct cgtcttctac cacgcggtac
     2821 aacgttggcc cgaggccttg tagctgttgc ccagtttcga aagaaggcgg attctacaat
     2881 ctggcgaccg ggcacttgtg ccgtcaccgg gtggtcaccc tccttgaaat tggctggcta
     2941 cgggtcgaac gatgagcagg gttcttatgt tgcactggcc gcagggagct gctacgcttg
     3001 cagcgaacaa aaggcgaata tcgtcgccga gagatccgat gaccacatcc cagcaagcct
     3061 tggccccacg ctcaagtacg actcttgata ccgttcacga cattctaggg acgctggttg
     3121 cccgctaccg caacgacgaa gtgctaacgt gcctgcgaca aatcccagcc cgcgaggaaa
     3181 attttcgccc cattccggac tgggtgagca gggaactatc tgacgcgtac cgggcaaaag
     3241 ggatcagaga gctctattca caccaggctg ccactgccga gctagtacac ggcggcagaa
     3301 atgtggtcgt ggtaacgccg acagcgtcgc gtggcgagat cgtgcaactc agctagttgg
     3361 tcttgggcta gcgctttcgt tggaaacagg taaagagcgc gggtatccgt gttctccagc
     3421 aatgcattca acactggaac atccccagtc acgtgctggg aaggcgactt tgtgcccaaa
     3481 cgtggggtgc acaggtcaca gacaaactcg acgcccagta gaaccattag cagcaggata
     3541 atggggagga catttccctt tcgcccgctc ccaaaggaag cacgtcatgc tgactggggt
     3601 ccacatcctg ctcacctacc agtgcacgag ggagtgcgat cactgctttc tgcactgcgg
     3661 gccacagcgc gaggggacct ttaccctccg ccaacttcgc gaactcctgc acgagatcaa
     3721 gaagattcac acaatcgaca cggtttactt cgaaggcggc gagccattcc tgttctacgc
     3781 ggttctgctg gtagggctgc ggatggtgca cgacgcgggt ttgcgagctg gcatcgtgac
     3841 caacggatac tgggcgaact cgctggagga tgcggagcaa tggctgcgtc ccttgctcga
     3901 cttgggcatt gctgacttca gcgtgagcga cgacgaattt cacagtacca agggcgacca
     3961 gagtcccgga gcccttgcgt accgcgctgc aagaaaactt ggattgcctt gcgcgaagat
     4021 ttgcattgcg gcgccggccg tctcgctggg gcatagtacc ggcagcgaga aaggtcagcc
     4081 ggtcacgggt ggtggagttc tcttcaaggg acgggctgcg gagaagctca ccgctggtct
     4141 gccagtgcgg ccctggcaga ggctaaccac atgtccgcat gaagaactcg ccaatcccga
     4201 acgagtacac gtggatacgt tcggcaacgt gcatgtttgc cagggagtga gtattggcaa
     4261 catgttgagt acgccgctct ctgaactcgt acgcacctat gacgcggcca agcatccgat
     4321 cgtgggccct ctggtcgagg gtggcccggc gcgcctcgtc gagcggttcg gggtagaagt
     4381 ggccgacgct tatgttgacg agtgccatct ttgttacacg gcgagaaaat tgttgctgcc
     4441 ggcctttccg caatacctcg cgccggcgca ggtctacgga ttatgaggat ctccatgcgc
     4501 aaaacggcct tactactcgc tctcattgtt tgcatcgctg ttcaagccca ggatctgaaa
     4561 tcgatccaac taccagcccc acagacggac ggcagccgtc cgctgatgca ggtgttgaag
     4621 gaacgaaaaa ctcaacggga gtttggtccc gagaagttac ccacgcaggt gctagccaac
     4681 ctcctttggg ctgcattcgg catcaaccgt cccgatggca gacgtaccgc gccgtcggcg
     4741 atgaactggc aggaaattga catctacgta gctctgggcg atggcctgtt tctgtacaat
     4801 gccaaagcga accgtcttga gccgatcctg gcgcaagata tgcgcgcggc cacgggaaca
     4861 caggcctttg tcgccgctgc acctttgaac ctggtttacg tcgcagatct cagcaagacc
     4921 ggacaatcga gtagtgacgc agagttcttc accgccgccg acgcaggttt catcagtgag
     4981 aatgtctatc tcttctgtac ttccgaggga ttggcaacgg tcgtccgagg atctgtggat
     5041 cgcgccgcac tggccaaggt catgaagttg agaccagacc agaagattct gctggcgcag
     5101 tctgtcggtt atccgaagaa gtagatgttt atcatcttcc tatggtcacc gaacacaggc
     5161 taggcgacat catagcgaac tccgtaaccc atggcgtggg ggctgcactc gcggttgccg
     5221 gagcgattac actggtggtc actgccgtag gcggaaccgc ctggcaaatt gccagctgct
     5281 cgatttttgg ctgcagcctg gtgatggtct atgtctgctc gacgctgtat cactcgctcg
     5341 tccggacccg tgcgcgtcac gtgttgcgaa tcattgatca ttcggcaatc tatcttctga
     5401 tcgccggaac ttatacccca ttcgtcctcg tgccgctgcg cgggaagctg ggatggcttc
     5461 tgtttgccat tgtgtggagc ctcgccatcc tgggaattgt gttcaagagt ttcgcagttg
     5521 accgattcgc catcgcgtcg gtcgtggtgt atctgggaat gggctggctc gtcgtgttcg
     5581 cggcgcgccc cttgctgcag gctctcacgt ggcacggcat attctggatt gcactgggcg
     5641 gactgttcta cacagcgggc atcgtcttct acgctctcga ccggctgtcg tacttccatg
     5701 cgttctggca tctctttgtg ctggccggaa gcacctgtca ctacttcgcc gtgttgtttt
     5761 atgttgccca gccacgcagc tgacgaggaa aggccccagt cgctgcttgc tgtctcgcat
     5821 tccttggatg aggtcttcga cgtgtggccg catgcctgcg tcggaccggc aaagctaaca
     5881 ctcagcaggt cacatggaga attgcgttgg tgctcgtggc gtgctgattc aagagctcca
     5941 tggcttcttc cgttgggagg atgagcaatt caacttttcg ccgcttagcc tcacgtttca
     6001 cctcatccat gactggtagc gctccttcgc ccgtaccgat ggagccattt gcgggggagc
     6061 cggcgcactc ggctgcaaca catgcaagac ccgaacctct atatgctcag ggcgaaactg
     6121 cgcgataaca gcttgtagta catctcctga gaacttcgaa tcgtcaattc ctgttaggat
     6181 tttcatgact cctcctagag agcagggtcg ccactgaccg caaagatcat gcagggatcg
     6241 tcacgccttc cggcgggctt tcgttcccgt cttgcgcgcg cctgttggct ctttcggagc
     6301 tgcgagacgc acgcattcag gagtcttccg caactcctta atacgccgcc aacaatcctc
     6361 acgggtccaa cccgggacaa gctcaaggat ttccttgatt gcatcgcgct ttttggcagg
     6421 cccgaggtta atcccagcca tcagcagttg atcaatggct gccaactgtt tgctgcccga
     6481 attgcctgat cggtgcagag ccccgcgcat gactgggtcc tgttatatcg cgtagcctga
     6541 gttgcggacc tcgaaatacc ccctcgcgaa atgtggtccc acacaagtca agtttcagcg
     6601 atttcagacg ttgtttcccc gtggaactga tctggtttga gctccgcaaa cgccagcact
     6661 tgcagagcaa gttagcttgc ggccagccta cgggacgaag ggacactctt aaggacacct
     6721 tcctctgcgc tctgcaacgc accatttaca gcattcttct atgcacagac agaagatgtc
     6781 accgatagat gctgccccgc aattccggat tcagtaagaa actattcttg ctaaatattt
     6841 atgcttccga ccgcgcgagg atcgccccgc catcatgcgc gtgaaggcac ttgacggcgg
     6901 actgagcctt aggcctcagc ggatccctgc gcgacggttc caggcgtgtt cgacctcttg
     6961 ctgaaacccg gctacactgc ctgccgagta tggctgccac gttacccatt ccgatctgtc
     7021 gcgagcgtgt agtaagtatt cagcaccacg aagatcctgc cggtttcgtg tcggctaatc
     7081 aggcgaaggt tctcttacgg ccctttcttg ccgattacat cggcattggc ggcacgggtc
     7141 actcctgaag atgcgcgtga agcaagtcga cccgcatgcc aacgtcattc gactggaacc
     7201 agggacgacg aagaacaaaa agggcttgga ggtaacgatg cctcgccgcg tgagggagct
     7261 gttgcttgag tgcatcaagg gcaacgcgcc ggaggcgtgc ctgtttacgc gagcgaacgg
     7321 cacaccggtt agagatttcc gtcgcagttg gagaaaggcc tgtgccgagg cgggcgtgcc
     7381 gggattgcac ttccatgatc tgcgcagcac cccgcccgga acatgcgacg cgggcacatc
     7441 tccgagaggg ttgctatgga gattggaggc tggaagacaa cctctgtatt tcatcggtac
     7501 gcgattgtgg acaatcagga cgttgctcgc gccatggaca tgttggaaag aagccaggac
     7561 caacaacgcc aagagctgga acgaacgctc gccgccaagc gtggccagcc gatagtgatc
     7621 aatgaccgca atgccacagg tgacggccaa acgaagaaaa gggcgaattt agctgggaat
     7681 tcttcactta cgtcacgttt acgtcacgtt gagggtctaa taggagtttc caaacagata
     7741 cgctgcggaa caaaacatga cgataaggca ttaaacaaca ataagttaag tggtgccggg
     7801 agggggactt gaacccccac aaccctttcg ggtctgcgga ttttaagtcc cttaactatg
     7861 cttgtaagtg actgcaccag agtgacttac tttttagaaa cgtgaaaatg tgtaagcgac
     7921 tgtgtaaatc tcggccaact tgaaatctct ctccaccgct gcatcatagc agccatagga
     7981 gcctccgttt actgtcggtt gttttccatg aaggaggcag cataggccgc ttctgctgcc
     8041 gctacttccc catctgccat ctgtgcaaac tccccttccc gcgcgcgctt tgagaggcgc
     8101 cccgcgttct gatgaagaaa gcggaacaac agatcgatgg tgcgctccgg catatcgacc
     8161 atcgcttcga tgcgggcgcg aaacccgtca taccgctcca agaactcggt ctcgcgcggc
     8221 aggtcctctt cgatcgtctg tttgacgcag gcgtagagga actcggcgtg gggtgtggca
     8281 tcgaaaaaac ggtagaagtc agccgtgtcg ttcaagactt tgacgttgct gtccgccgtg
     8341 ggctcccagt cgatcatggg caacagacgc ccggaatagt cttccagagt ggcacggtag
     8401 tcgtcgattc gcgccaatat tgccgccgaa acaggaaaca tgacgcccgc cgggttgaat
     8461 ccgcgttgag ccagaacgtg atggatcagg tagcggtgaa tgcggccatt gccgtccacg
     8521 aacggatgga tataaacaaa cccgaaggcc agaacggcgg cagcaatcac cgcgtccaga
     8581 tttagcgccg gcccgcgatc aaaagcgccc atgccgctta tcaacgaccg aagatcctcc
     8641 gggcgcgcgc tgatatggtc cggaatcgga aggcgggttt cccgatcatg ttcgccgacg
     8701 aatccgccct cttcccgaag gcccagctta acgaagcgcg cgtcgccgat gacgatggtc
     8761 tgcagccgta gcaattcgtc cagctcgata gatttttttc ccgcttcgcc tatcgcgcgc
     8821 ccccaacgct ggattcgatc ttgaggcggg cgctctcctt cgatggcgta gctcgctctg
     8881 gaatccttca gcagcagaaa ggccgcggtt cgcgcaagaa cgtccttcgg cacgttggcc
     8941 acaacttcct gcgcccggtg gacgagatcc atggcagcga attcttcaag cgatttcgtc
     9001 cggaagacca tcgggcagaa ttccggggta cccggcaggt tgttgcggac gcgatggcgg
     9061 ggtgagtttt cgcccggcac agcccattgc tgctcagtac ccacgaccgg ctcatatgtg
     9121 cccttgtcgg cgtcgggtag atcgagccgc ctcccgatga gccactcgta aaggaaccaa
     9181 atccgccggg agtagctacc ggtcggcttc gcccgcacaa tcgtctctat ctcggcaggg
     9241 gccagcgcgg cgaacagccg tttcagaaca gcgagatcga ggccctcgta tttcagggcg
     9301 aaggtaagat gcccttccag attgggtgat ggagcatggc ggggcgtgag gatgcgccaa
     9361 cccgcttcgt tcctcgtgcg atggtgctca cccgtcgcgg agagcgttcg tggcaggggt
     9421 acccgcagac cgtaagcatc gataagggca gaataacccg ctggtgtcgc cctctccggc
     9481 agcgtccgtt cgtgaaaaat cgttatcggt cctgaaaaat gatgttgagg agccatgcct
     9541 gtcgaaaacc ccgtcaccca tatgaaatat ggtttctatg ctgcgtatcc atgaaaaaca
     9601 atgatacagc atgaaaaaca atgccgcaat tggtattatt gtggaaactg gttatctttc
     9661 aggcctgtgg tcgctaaggc ccccctgccc gccccttcgg cggggtgctg gtttattcgg
     9721 cgtggtgtgt ctgcgccgga gatcttttgg gcttttcgtg tgctgcgctc tcgcgcttga
     9781 gggccgcaac cgcccgttcg cgtgtttcgc ccaaaaccca gcccctcggc gctcaccagg
     9841 aatgacccat atctgctttc caagaatgac ccaggaggtc tacagctgag tctaagagta
     9901 ggtcactacc ttccagatgg cagtatccga gtaggggctg caaacgatcc gcagcgggat
     9961 cgctaaacac tggccggcaa ggcagggtca agcgcctcga ttctgtgtgc aaaatacgcg
    10021 ggccccattc gcagaatcta gtagcgtggt ctgcatgacc gtcacctccc gcctctttga
    10081 tgcctatctc aagtgcccca ccaaatgttt cctttggtcg cgtggcgaaa cgggaacgag
    10141 caacgcctac gcagactggg cccggattca gaacatctcc tatcgtagcg aggggatcag
    10201 acgtttgaag gacggagtcg cgagcaatga atgtgttgcg gagccgctcg acgggaaaaa
    10261 cctgaagtcg gcgaagtggc gcttagccct agacagcaag gcgtacgcgg agaatctcga
    10321 atcggcaatt gacgctctgg agcgggtgcc acgggacacg ccaggcaaac cgccgcagtt
    10381 cagtccgatc cgattcgtct tcagaaacaa acttaatagg cacgacaaac tcctattggc
    10441 attcgatgcg ctcgtgttgt cggaagcgtt gggccgtgcc gtggatttcg ggaacatcat
    10501 ccacggcgac gccttcgtca atcttcgagt gaagacctct gccttgggga acgaggtgcg
    10561 gaagatcacc gcgaagattg ccaccttgct ctccggcaaa gcgcctgacc ccgtcctgaa
    10621 ccggcactgt gccgagtgcg agtttcggga ccgctgcaag cagaaggcag ttgagacaga
    10681 cgacctcagc ctcctggctg gtatcacgga agacgaacgc atcaggtatc ggagtaaggg
    10741 gatcttcacc gtcacacagt tgtcctacac gttccgcccg cgcaggactc cgaagagagc
    10801 caagaaccca ggcaagcccc gctacaacgc actgcaagcg ctcgctattc gggaaaacac
    10861 cgtctacatc cacggcagtc cccaaatccc cgactccaag gcccaggtct atttggacat
    10921 cgaaggactg ccggatagcg actcctatta cctcatcggc gtcctaatct tatccgaagg
    10981 acaggaaaca tttcactctt tctgggcgga ccagcgggaa caggaacccg agattttcgc
    11041 tcgactcgca aacaccgtct gcgagttgcc tgatttccga gtactgcact tcggaaagta
    11101 cgaaaccgcg gccttggaaa ggatgaagac aaggctgccc gattccctcc atccgaaact
    11161 cgatatgatt gtggcgcgag cgacgaatgt gctgtcggtc atccattctc acgtctactt
    11221 cccaacctat tcgaacggcc tcaaagagat tggaaggttt cttgggttct tgcgagacga
    11281 tgaaaacgcg acaggactgc aaagcatcat ctggcggcag aaatggcaag aaaccaaggt
    11341 tccagattgc agagcccgcc ttcttcaata taaccaggat gactgccgtg cattgaaggt
    11401 cgtctgtgac ttcatccggc ggctgaactc gccggaggcc gcaaccgccg cagtcgctca
    11461 gactcctttc aagaccactc acactgagga actgactaag gaccgcccac gctgggaatt
    11521 attcaggccg aggaaggaat attctaccga agacctcaag aaggtcgccg agtgtgccta
    11581 cttcgattac cagcgtgaga aggtattcgt acgcacgcac cctcatttca atgtgattaa
    11641 caaacagcat cgaaagcata atcgaacctc gtttcgaatc aacaaacatt actcaatgga
    11701 gagccagcga tgcccccggt gcaacagtcg caacattcag aaaggtcgcc aaagtagcca
    11761 cgagttggta gatctgaagt tcttcaaagg cggcgtgaag aagtggatca ctcggactgt
    11821 gtcatggaag tactcttgct ctaaatgcaa acatgcgttt agttccgccg agccgcgcga
    11881 tcgctacaaa ttcggtcacg ggcttctgag ttggtgcgtc tactccaatg ttgcctgcgg
    11941 cttgaacatg ggacgcgtcg aacggagcct ggaggatgtg tttggccttt cgttcaacca
    12001 gagccagttc ttcaggtcaa agcgctacat tatggctctc taccggccgc tttgcgaaca
    12061 gatcctcgaa agcatcctgg caagcccggt tatccatatt gatgaaacca cagtacgact
    12121 ccagaagcag acaggttatg tatgggtcat gactaccctc gacaaggttt actacttcta
    12181 taagtcatct cgggaagggt cctttctaca agagatgctc gctaagttct caggcgttct
    12241 gatctccgat ttctattcgg gatatgagtc tctcaagtgc aagcaacaaa aatgcctggt
    12301 acattttgtg cgcgacatcg atgatgacat cctgaagaac ccgctggatg tcgaactgaa
    12361 gagtattgcc caagagtttg ggctcctgct gagaacaatt atcgagacgg tcgatactcg
    12421 cggcctcaag acccggtacc tgcgcaaaca caagcgttct gtcgttcggt ttctggattc
    12481 agtgacatct acggacgttt tgtcgccact ggccaacaag tacaagaagc ggtttcagaa
    12541 gagcggcgag aagatgttca cgttcctaga tcatgacggt gtgccgtgga acaacaacaa
    12601 tgccgagcat gccatcaaac gctttgccag gtatcgccgt gacgccgatg gtcgtttcac
    12661 ggagcgcaca ctgcaggagt acctagtgct ggcaactgtc ttggaaacgt gcgagttcaa
    12721 taacctaaat gttctgaagt tcctgctctc gaaggaagcg actttggaag ggctgctcag
    12781 aatggccggg cgtaagactg accttgcccg cgcaaaacgt atcccttagc tgaatgattg
    12841 aaaatcaaca agggggacga aaatgactaa gtctacgcgg acgcggtaca cgctggagtt
    12901 caagcaggag gcggcgcggc tagttgagtc gggccagagc atagcggcag cggcacgcag
    12961 tctgggcgtg gttgagcaga cgctgttcaa ctgggtcaag gcgcagcggg aaggcaggct
    13021 caagggtgtc gacagcagca aggcggtaag cgccgagcag atggagatca gccgtttgcg
    13081 agccgagttg gggcgcgtca agatggagcg cgacatcctg ggaaaagcga cggcgtactt
    13141 cgcgaagggc cagaaatgaa gtacgccttt at
//
LOCUS       DASRKK010000775         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_84926, whole genome shotgun sequence.
ACCESSION   DASRKK010000775 DASRKK010000000
VERSION     DASRKK010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84926"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="hemG"
                     /locus_tag="VFC15_18660"
     CDS             complement(<1..1001)
                     /gene="hemG"
                     /locus_tag="VFC15_18660"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZM12234.1"
                     /translation="MKRIAIIGGGISGLSAAFYLEKARAAGADLEYTLFESGQRLGGS
                     MYSDRVEGCLVEAGPDSFLTEKPWALSLCKDLDIADQLTGSNDAQRKTYIVVNGRLVV
                     MPDGLMFMVPTQLVPTALSPLFSWSTKIRMARELLHPPRPMQTDETVAQLVERHFGAE
                     VVDRLADPLLSGVYGGDAAKLSARAVLPRFVEMEEKYGSLSRAMLAAHKKMMAARKGP
                     ARPLFTSLQDGMQQMVDAIADRLDPESIRLRTHILRVYPEDSSSSWRVSVEMNGEERF
                     DAVLIATPANVAGTLLDGVDRGLARNLLDITYSSSVTVTLGYYMDQLAVLPPGFGFLV
                     "
     gene            complement(1238..2152)
                     /gene="hemH"
                     /locus_tag="VFC15_18665"
     CDS             complement(1238..2152)
                     /gene="hemH"
                     /locus_tag="VFC15_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZM12235.1"
                     /translation="MSQPAVLLIAHGSPERVEDIPEFLLNISRGRPMPEAVVREVQHR
                     YSLIGSSPLTRITRQQADGVARELGLPVYVGMRNWHPFIADTLQQMAADGITSVVAIC
                     LAPHNSRTSVGLYKQVLFGNEPAFAIDFVEEWHDRPMLIAAFAEKLRAGWTKACAEHG
                     SQLPVLFTAHSVPLRTVTEGDPYETQTRETAALVAAQVSEIGAWRFAFQSQGMSGGEW
                     LGPTVEETIVSLRESGLAGVFVQPIGFVCDHVEILFDVDILFRQFAGERGMKLYRAES
                     LNDSPLFVRAVADLARSRVMTLNKVTFG"
     gene            complement(2149..>2781)
                     /locus_tag="VFC15_18670"
     CDS             complement(2149..>2781)
                     /locus_tag="VFC15_18670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZM12236.1"
                     /translation="APFTLASYMVEGGGSRHYLEVKKLMYNSPEVWDELVTKLTDVLV
                     EYAAAQVRAGADVLQIFDSWVGCLSVEDYRRYALMPTTQLIRRLQKATHVPIIYFGTD
                     SATLLPSMAETGAEVIGLDWRIPLDEGWRTVGHAHAVQGNLDPVTLFASWPELKRRAE
                     HILRLADGRPGHIFNLGHGILPHTPVTNVKDLAQFVHEFSLTAASPARIE"
BASE COUNT          564 a          891 c          818 g          508 t
ORIGIN      
        1 accaggaaac cgaatcccgg cggcagaact gccagttggt ccatgtagta gccgagggta
       61 accgtgacgg acgagctgta ggtaatgtcc aggaggttcc gcgcaaggcc gcggtccacg
      121 ccgtccagca aagtccctgc aacattggcg ggagtagcta tgagcacggc atcgaagcgt
      181 tcctctccat tcatctcgac cgatacgcgc cacgacgaag acgaatcctc gggataaacg
      241 cgaaggatgt gcgtgcgcaa gcggatggat tcggggtcca agcggtcggc aatggcgtcc
      301 accatctgct gcatgccatc ctgcaaggag gtaaacagcg ggcgcgccgg ccccttacgg
      361 gcggccatca ttttcttgtg cgccgccaac atggcccggc tgaggctgcc gtacttctcc
      421 tccatctcga cgaaacgcgg caacaccgcg cgcgcgctga gctttgcggc gtctccgcca
      481 tagacgcccg acagcagcgg atcggcgagg cggtccacca cctccgcgcc gaagtgacgt
      541 tccaccagtt gcgcaaccgt ttcgtcggtc tgcatgggcc gcggcggatg caaaagctcg
      601 cgggccatcc ggatcttggt gctccacgag aacaagggcg agagcgcggt cgggaccagt
      661 tgggtgggca ccatgaacat aagcccatca ggcatcacca ccagtcggcc attcacgacg
      721 atgtaggttt tgcgctgggc gtcgttggag ccggtaagct ggtcggcgat gtcgaggtcc
      781 ttgcacagag aaagcgccca tggtttttca gtgaggaagg aatccggccc agcttccact
      841 aggcacccct ccactcggtc ggaatacatc gatccaccga gccgctggcc gctttcaaag
      901 agcgtgtact ccaggtctgc tccggctgca cgcgccttct ccagataaaa tgcggcgctc
      961 agcccggaaa tgcctccgcc gataatggca atgcgcttca tacggttttc ttcatcgaat
     1021 cttcaggctg ctgaaagcct cttccaatgt acctcggggt aaaatcagct tcacatctgg
     1081 tgtataggcg atgggtggag cacgcctttc aggcgtacat aactgccgtc agttcctggg
     1141 tcgcgcctta gcgcctgagg taatgtttcg gtcacctcag cggctaaagc cgactcattc
     1201 ccgaatctta attgcaccgc tgaagcggtg ctccacccta cccgaacgtc actttgttca
     1261 gagtcataac ccggctgcgc gccaaatcgg cgacagcccg cacgaacagc ggcgaatcgt
     1321 tcagcgattc ggcccggtag agtttcatgc cgcgttcgcc ggcgaattgc cggaacagga
     1381 tatcgacgtc aaacaggatc tccacatgat cgcagacgaa gccgatgggc tgcacgaaaa
     1441 cacccgctag gcccgactcc cgcaaactca cgatggtctc ttcgacggtg ggaccgagcc
     1501 attcgccgcc cgacattccc tggctttgga aagcgaagcg ccacgcgcca atttcggaga
     1561 cctgcgcggc aaccagcgcc gccgtctcgc gggtctgcgt ctcgtaggga tcgccctcgg
     1621 taacggtacg caggggaacg ctatgggccg tgaacagcac gggcaactgc gatccgtgct
     1681 ccgcgcaagc tttcgtccag ccagcgcgca gcttctcggc aaaagctgca atcagcatcg
     1741 gacgatcgtg ccactcttcg acaaaatcga tggcgaacgc aggctcgttc ccgaacaata
     1801 cctgtttgta aagtccgacg cttgtgcgtg aattgtgggg agcaaggcag atggcgacca
     1861 cgctcgtaat gccgtcggca gccatctgct gcaacgtgtc cgcgataaac ggatgccagt
     1921 tgcgcatacc aacataaacc ggaagcccca actcgcgcgc caccccgtcg gcctgctggc
     1981 gggtaatcct agtcagcggc gagctgccaa tcaacgagta gcgatgctgc acctcgcgca
     2041 ccaccgcctc cggcatgggc cggccgcgcg agatgttcag caggaactcg ggaatgtcct
     2101 ccacccgctc cggactgccg tgagcgatga gcagcactgc cggttggctc attcgatcct
     2161 cgcggggctg gccgccgtca agctgaactc atgcacaaat tgcgccaggt cctttacgtt
     2221 ggtgaccggc gtgtgcggca aaatgccatg gccaagattg aagatgtgcc cgggccgtcc
     2281 gtccgccagg cgaagaatgt gttcggcccg ccgcttcagt tccggccacg acgcaaacag
     2341 cgtcacggga tcgaggttgc cctgcacggc atgcgcatgt cccacggtgc gccagccttc
     2401 gtcgagggga atccgccaat ccaggccgat gacctccgct ccggtctccg ccatggaggg
     2461 caacagggta gcgctgtcag tgccgaagta gatgatcggc acgtgggttg ctttctgcaa
     2521 ccgccgaatg agctgcgtgg tgggcatcag cgcgtagcgg cgataatctt ccacgctcag
     2581 gcagccgacc caactgtcga agatctgcaa cacgtcggcg cccgcgcgta cctgcgcagc
     2641 ggcgtactcc accagcacgt ccgtcagctt ggtcaccaac tcgtcccaaa cctcgggcga
     2701 gttgtacatc agcttcttca cttccagata gtgccgcgac ccgccaccct cgaccatgta
     2761 gctggcgagg gtaaacggcg c
//
LOCUS       DASRKK010000776         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_769374, whole genome shotgun sequence.
ACCESSION   DASRKK010000776 DASRKK010000000
VERSION     DASRKK010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769374"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFC15_18675"
     CDS             <1..950
                     /locus_tag="VFC15_18675"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZM12237.1"
                     /translation="RIGIKRFRYMCENFLPQRHQKWAKDLRELQDALGEVHDFDVLKA
                     TIKAQPGIEAGDRERWNKRIAEERQQRLDLYRQKMLGKNSLWPVWRAELPSGPSLEQA
                     AMEKLRTWASFLDPDVNHSAHVTRLALQLYDGLSKNGTVTAGAEYRRILEAAAILHEV
                     GRSRGPEGHRKRAYSMIRRLKPPLGWSAEELQCVAVIARYHGGMLPQTSNSAFVGLTP
                     KRRKALLPIAGILRLADAFDFFHDRKIDQVSVERRDGMVIVYGRGLQGISPVAERLAR
                     ARYLLETTCGCPIMIRPQAIKPATASHAAGHARELAAGS"
     gene            complement(970..1194)
                     /locus_tag="VFC15_18680"
     CDS             complement(970..1194)
                     /locus_tag="VFC15_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12238.1"
                     /translation="MISTSDSATEADHRERIWFFPDKFWRATENMPPDEVANLMAEVE
                     GYAAAGDVQALRKYPFVVVGDPYKKKKSVA"
BASE COUNT          560 a          731 c          736 g          507 t
ORIGIN      
        1 tgcgaatcgg catcaagcgg tttcgctaca tgtgcgagaa tttccttccg cagcgccacc
       61 aaaagtgggc caaggacctg cgcgaactcc aggacgctct cggcgaggtc cacgatttcg
      121 atgttttgaa ggcgacgatc aaagcgcagc ctgggatcga ggccggagac cgcgaacgct
      181 ggaacaagag aattgcggag gaacggcagc agcggctgga tctttatcgt cagaagatgt
      241 tgggcaagaa ttcgctctgg ccggtgtggc gcgctgaact gccgagcggt ccctcattgg
      301 aacaagccgc catggagaaa ctgcggacct gggcctcctt tctggatcct gacgtgaacc
      361 actccgcgca cgtgacgcgg cttgcgctgc aactttacga cggcttgtcg aagaatggga
      421 ccgtgaccgc tggagcggag taccgccgca tcctggaggc cgcagccatc ctgcatgagg
      481 tggggcgaag ccgaggccct gagggccatc gcaagcgcgc ctattccatg attcgccggc
      541 tcaagcctcc cctgggatgg agcgcagagg aactccagtg tgtcgccgtg attgctcgct
      601 accatggcgg catgcttccg caaaccagca actccgcatt tgtgggactt accccaaaac
      661 ggcgaaaggc cctcttgccc attgcaggca ttctgagatt ggcggacgct ttcgattttt
      721 ttcacgaccg aaagatcgat caggtgtcgg tagaacgacg ggatggaatg gtgattgtct
      781 acggacgagg cctgcaagga atcagtcctg tggcggaacg gctagcccgt gcccgatacc
      841 tgctggaaac gacctgtggg tgcccgatta tgattcgtcc tcaggccatc aagccggcga
      901 ccgcatctca cgctgcggga cacgcgcgtg aactggcggc cggttcatga gggaagactt
      961 gatcctgaat caggcaaccg atttcttctt cttgtagggg tctccaacaa ccacgaaagg
     1021 atatttgcgg agcgcctgca catcgcccgc ggcagcgtag ccctcaactt ctgccatcag
     1081 attcgcaact tcgtccggag gcatgttctc ggtagcgcgc caaaacttgt ccgggaaaaa
     1141 ccaaatacgt tcgcgatggt cagcctcggt agcactgtcg gaagtcgata tcataaatct
     1201 cctcccacaa tgtgggatat gcagaaacca cggtgagttt gaagaataag tgccttgatc
     1261 gactgattgc tcaaacatga ttatggaccg cggctgtccg cttgtaaacc gccaatagag
     1321 cgcgcatgtg ctttgcattc ccgtttctgc gccaacgccc acctccccac tagcctaact
     1381 tcaacaaact aaaaggaggc gatcttcttg cgagagcaag gagtggcgcg cccgcaaact
     1441 tggcaggcta cggctccgcg ggcagcgggc gctttatccc gtccgaacgc tcattgggct
     1501 acaatcctgc aagcgttgag atgcacttcg ctgcagtcct tcgcgtgtcg cacattgtgg
     1561 gatatggacg atctggaata gtaagtgcga cgcaccgtac gggctatacg ggtttcgcgg
     1621 gttgcctaga actgacagga atggagtatg cctttagaca ctgcgtcgca gctccgttag
     1681 agcgattcca gagtggctgt acctcgggta gtgcgggcct tcaggcccgc gttagttacg
     1741 ccctcgcaat tcatggcttc agccgctgag gtcatcgacc tcagcggcta aagcgatttt
     1801 cctctcgccc ttgaacgctg gcccgaaggc ccgcactacc cgaacgagcg ggatacacca
     1861 atcctggaac cgctgtggcg tggcaagggg caggaagttg cgaaaaccag ccgcgcggcg
     1921 acggcatcga aattaaaccc caggcgtgaa gctcggcggg aaagtgcgtg tgctccgcga
     1981 tgaacgatat gtgccgctaa ttatgacgat tcctttgggc cgagtggcac acgtgggtca
     2041 agcggtaaat tgaccagagc cgcgtagcgg tggcacagaa cgacaccgtc tcttcacgtc
     2101 tgctagcccg gcgaaccggc tttcgccggt gaggagcgat tcccaatgtt cactctgaaa
     2161 taggtccaac taaatcccct ttccatagag cggcacgaac ggacctgcac gaccgaaggc
     2221 ccatacgctg ccgagttttt cagtggatcg tcgctagcgt tcgaggcgcg caccaaaaaa
     2281 aacgccacgg gaagtggcgt aaacttgcgg tcaacaaaga tcgttcgtga ccgtgatgca
     2341 gaacggcatc caccgcttta gggcagatgc cgtttttgca cacttcgagt ttcgcttcta
     2401 cgcctgaccg tcaacagcgg tcccgtttcg ccactaatag acgaacccag cggtgctagg
     2461 cgcggaactt acggcgaact acgccagcga tcccgagaag cccgctgccg aacagaacta
     2521 acgtgcccgg ttca
//
LOCUS       DASRKK010000777         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_378229, whole genome shotgun sequence.
ACCESSION   DASRKK010000777 DASRKK010000000
VERSION     DASRKK010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378229"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFC15_18685"
     CDS             complement(<1..312)
                     /locus_tag="VFC15_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12239.1"
                     /translation="MYLTEIDGSKAEIKLIPADAAQAQIQLSRWELASKLSHPHLLRI
                     LHTGRWRADDERDMYFAVMEYADENLAQILPSRLLTPAEASEMLIPTLDVLDYLHGQG
                     MV"
     gene            1676..2392
                     /locus_tag="VFC15_18690"
     CDS             1676..2392
                     /locus_tag="VFC15_18690"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZM12240.1"
                     /translation="MIFRMVTENPTWGAPRIHGELLKLGFDLSERSVSRWIQRVPRDL
                     DVVKRWLTFLRNHREAIAAMDFFTVPTLTFGVLYCFFVIGHDRRKILHFNVRRNPNAL
                     WIVQQLREAWAYQQPHRFLLFDRDAKFGAEVVSAVREMGSQPTRTAFRSPWQNGIAER
                     WVGSCRRDLLDHVILLNERHLKRLMSLYLRYYHEDRTHLGLGKDTPVGRVAASVSPSG
                     KKIISLPRLGGLHHRYAVAA"
     gene            2755..2979
                     /locus_tag="VFC15_18695"
     CDS             2755..2979
                     /locus_tag="VFC15_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12241.1"
                     /translation="MEVDFTPDLQAKLTRLAAEQGRDTKSIVREAVKRFVDYDEWFLR
                     EVEKGLAAADRGELIDHDEIGKLIDSRYPG"
     gene            2979..3257
                     /locus_tag="VFC15_18700"
     CDS             2979..3257
                     /locus_tag="VFC15_18700"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM12242.1"
                     /translation="MRIRWTEPAAHDLTHICDYTEEHDGPAAARRVALTIYQGVSSLT
                     QFPHRGRQGRKVGPRELVFSGLPFLAVYRIRAEVIEINRILHGAQKWP"
     gene            complement(3311..>3599)
                     /gene="ruvX"
                     /locus_tag="VFC15_18705"
     CDS             complement(3311..>3599)
                     /gene="ruvX"
                     /locus_tag="VFC15_18705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZM12243.1"
                     /translation="VIKQYHIAELVMGLPLRMSGSEGIQSEKVQVFAEELRRKFKLPV
                     HLFDERLTSVEANRLLRETDMGIRRRAGVVDQLAAVLILQSFLEFRKNRNL"
BASE COUNT          746 a         1037 c         1022 g          794 t
ORIGIN      
        1 caccatgcct tgtccatgca agtagtccag gacatccaag gtaggaatca gcatttcgct
       61 ggcttcggcc ggcgtcagca gtcggctagg aagaatttgt gcgagattct cgtcggcgta
      121 ttccataacg gcgaagtaca tgtcccgttc gtcgtcagct cgccagcgac ctgtgtggag
      181 aattcgcaga aggtgaggat gggacagctt gctcgccaac tcccaccgtg aaagttggat
      241 ctgtgcctgc gctgcgtccg ctgggattaa cttgatttcc gctttcgagc cgtcgatttc
      301 tgtgaggtac acggcgctct gttccgagcc gccaagatac cgaaggagtg gaagtttccc
      361 gttaacgacc tgtccttccc attgctgcca cgtctccatg cttgtactac cccgttgttg
      421 tgacataagt aaacccggaa ttgtaacgac aggcaactgg ggtgccggag aactgaggtg
      481 aaacgagaaa acggtggcaa gatcaacttg taagcggatc ctgcttggcc tcaaacgccg
      541 agacaatagt ccctggcttt cttttgctcg ctcaccgagc ctcgttcttg ataacgcggc
      601 actgaacttt catgctgcac aaaaattggg gaatgtaccg attgtggaat cactctggaa
      661 gcagaactta caactcacgt cttctttggc gaaccaaaaa tccgggccac acggacaagg
      721 actctggccg atatcctctt ccgcatggcc cgattgcgcc acttcgtctg cctttacaca
      781 tagcattcga aggaccaaac gggttgtcca agaacagggg acttagcgta ttcgctgccg
      841 caagagtcgt gtaaaaagcg cacagggttc cgtagatatt tccgaatggc ccgcacgcaa
      901 aacttttcgc tcggaccggc cgcttacttc tttcgagcct caccgagcgt ctcggctcga
      961 agcagcccct cgtctcgatg cttgtgttgg gaggtgcgac cagccgacca gggagatggc
     1021 gaacccgcag tgcctgatac gggctcgttt gccaggctga tcgggaacgg cttgccgcag
     1081 ctcttgcatc caaatcgttg gagtccagac gtaggagctc ggaaggcgtg tgcacgaaac
     1141 ctcggcccac acggaagaat gctcgggccg catccactcg tgcgagactc gaccgtggtc
     1201 agttcttggt cggaactgcg gccatgcgag cttgcatctc gcgggttaga tgcggcacca
     1261 ctgtgcgctc gccaaaacgt cgtacaccta caaaatgaat gacttgccgc aaagctcgag
     1321 tagtttaggg ctgttccgat aagattctgc catgatcgtg tcgctccggc atctcctcgg
     1381 gtggatggtc agcgctttcc gttcccgcga ggacctcgtc ctcgaaaacc tggctctgcg
     1441 tcagcaattg ctggctttac acgcgcaacg acctcgccgt cgattgaccg ccctgcacaa
     1501 gctgttctgg gttgcgttgc aaaccttccg gtccgggtgg aggaagcctc tcatcttggt
     1561 tactcctaga accgtcgtga actggcatcg gacggggttt cgtttgtatc ggagattgct
     1621 ctcaagagtc agaccaatag gagggcggaa gcctgtcagc aaagaggttc gcgccttgat
     1681 cttccgtatg gtcaccgaga atccgacgtg gggagcgccg cgcattcacg gcgaactgct
     1741 caagctgggt ttcgatctgt cggaaaggag cgtctcgcga tggatacagc gagtcccgag
     1801 agacctagat gtggtgaagc gatggctgac gttcctcagg aaccatcgcg aagccattgc
     1861 ggcgatggac ttcttcaccg tcccaacgct cacgtttggt gttctgtatt gctttttcgt
     1921 gatcggccac gatcggcgta agatcctgca cttcaatgtg aggcgaaatc ccaatgcgct
     1981 ttggattgtg cagcagttgc gagaagcctg ggcctaccaa cagccgcacc gattcttgct
     2041 cttcgaccgc gacgcaaagt ttggagccga ggtggtttcg gcggtgaggg agatgggaag
     2101 ccagcccacc cgcacggcct ttcgcagtcc ctggcaaaac gggattgcgg agcgctgggt
     2161 aggaagttgc cggcgcgact tgctggacca cgtgatcctc ctgaatgagc ggcatctgaa
     2221 acggctgatg tctctgtatc tgcgctacta ccacgaggac cggactcatc ttggccttgg
     2281 gaaggacaca cccgttgggc gagttgcggc gtcggtttcg cccagcggaa aaaagatcat
     2341 ctctttgccg cgactcggcg ggttgcatca ccgctacgcg gtagcggcct gagttacctc
     2401 tgaccaccaa ccgcaactgt ctggggcaga ctcgtcaaag ttgtagaggt attcacgcgt
     2461 gtattgcgag ttgagccccg ggaatcgttt ccacgacttc gcagggccct tgacgacttg
     2521 atgctcaata ccgcttgccc gttttatggg tagcatcgtg gggcaagaaa ggcccgctct
     2581 tagttatggc gaggcacaac agttatttga gtcgctcctg acgccgcgag gatcgccgcc
     2641 ctgcggggcg atgggagtcc ccattaacag gggttagggc aacactcagg ccgccaacac
     2701 cagccgccaa caccaggctt gacacttatt tttccgcgtc ttaccctcaa ggctatggaa
     2761 gtagacttca ctccggatct ccaagccaaa ctcacccgac ttgccgccga gcaaggccgg
     2821 gacacgaagt caatcgtgcg agaggccgta aagcgtttcg tggactacga tgaatggttt
     2881 ctgcgggagg tggagaaagg tctggccgcc gccgaccggg gggagttgat tgaccacgac
     2941 gaaataggca agcttattga tagccgatat cccggctgat gcgtatccgt tggacagaac
     3001 ccgccgccca cgatctcacc cacatctgcg actacaccga ggaacatgac ggcccggcag
     3061 cagcccgacg tgtggccctc acaatctatc aaggcgttag ctccctcacg cagtttcccc
     3121 accgcggtcg tcagggtcgc aaggtgggcc cccgcgagct ggtattttca ggtttgcctt
     3181 ttttggccgt ataccgaatc cgcgcagagg tcattgaaat caaccgcatc ctgcatggcg
     3241 cacagaagtg gccctagtta ggggtgccca tagtctatga gaatccgtgt tctttcgcgc
     3301 tgatccgcgg ctacaggttc ctgtttttcc ggaattccag aaacgattgc agaatcaaca
     3361 ccgccgccaa ttggtccacc acccccgcgc ggcggcggat gcccatgtcg gtttcgcgca
     3421 acagccggtt ggcttcgacc gaagtcaacc gctcgtcgaa gaggtggacc ggcagcttga
     3481 acttgcggcg caattcttcg gcgaacacct gcactttctc cgactgaatc ccctcgcttc
     3541 cgctcatgcg caggggcagt cccatcacca gctcggcgat atggtattgc ttgatcact
//
LOCUS       DASRKK010000778         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_865996, whole genome shotgun sequence.
ACCESSION   DASRKK010000778 DASRKK010000000
VERSION     DASRKK010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865996"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFC15_18710"
     CDS             <1..229
                     /locus_tag="VFC15_18710"
                     /inference="COORDINATES: protein motif:HMM:NF022083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZM12244.1"
                     /translation="MNSDHKDALILLAKRFAGIDAQEAAMTSVDRLGFNVRLKAQDGV
                     HGARVAFLREVTNPTQTREVIVEMVKQARQV"
     gene            1156..1893
                     /locus_tag="VFC15_18715"
     CDS             1156..1893
                     /locus_tag="VFC15_18715"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12245.1"
                     /translation="MNGILVRIGVDQAYGKWNAPVDEATHEFVFVPIPDGDGKQYRPG
                     LRVPYDNIRADVRIFAQQKNFPADWEFGFPEALRQHSAHLDPDFRWLTYGDNGAVRGK
                     GIRQLKRGDLLVFYSGLRSIQRQNVLVYAITGLYVVDEVARAAEIPAERHRENAHTRW
                     DVISPNDIVVIARADVSGRLQRCIPMGEFRDRAYRVTPDLEAAWGGLSVKNGYVQRSV
                     VPPSFRDAGRFYDWFLQQDVSLVKRNN"
     gene            1894..2526
                     /locus_tag="VFC15_18720"
     CDS             1894..2526
                     /locus_tag="VFC15_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12246.1"
                     /translation="MPECHVLIVHLRRPDRSRPDEKRTDPFWEFGSFGITTCHAKNLL
                     KPGNVKRLKGVRFAFAQGGKNEMKLVLLTPPIRRIVEYKDRSEVLWDRRFPFKFRNAP
                     LLIDSDGKSDFPSLKNHLKGVRRSTWVARFSSKFRSRTTYLDPEFANEIMAVFDAKYK
                     KATREDLATLYEDALPYPPHCIDTNREKTYERCQKGAVRKGSGCGVRRRL"
     gene            2840..3040
                     /locus_tag="VFC15_18725"
     CDS             2840..3040
                     /locus_tag="VFC15_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12247.1"
                     /translation="MSPAARKRISEAMKQRWAKWKGKSAPKQDKTTPKKTTVRRPMSA
                     AAKKKLSALMKARWAAKKEAKA"
     gene            3041..>3511
                     /locus_tag="VFC15_18730"
     CDS             3041..>3511
                     /locus_tag="VFC15_18730"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZM12248.1"
                     /translation="MSSSSFGKHASGATSGQSAVPEPSFAERARTLMHSSRIGSLSTL
                     SRKQPGFPFGSLMPYALDGQGRPIFLISTMAMHTQNLLADPRASLFMTEPEASGDPLG
                     ASRATLIGSVSRIPEPDLAAARTVYLTRYPDSKHWVDFDDFFFYRMDVLDVYYVG"
BASE COUNT          828 a          939 c          998 g          746 t
ORIGIN      
        1 catgaattcc gatcacaagg atgcgctcat cctgctcgcc aaacggtttg ctggtattga
       61 cgcgcaggag gcggcgatga cctcagtcga ccgcctcggc tttaacgtcc gcctgaaagc
      121 tcaggatggc gtgcatggag cccgcgtcgc tttcctgcga gaagttacga atccgacgca
      181 gaccagagaa gtgattgtcg aaatggtgaa gcaggcgcgc caagtttaag ccggctggcg
      241 ttcgcggtgg acttgtccag gtctgtaaat gtgcagctac tcctgtgcac ttcggggaga
      301 aggcgaacaa gcgatctgcc accgtacccg aaaaggaaaa ggctgtgact ttggttatgc
      361 tctagccgac aactcccgcg agagagtcac cagcgtggac gcatgtaccg tcacagccct
      421 tgatgcgatc accgctttcg cagttccaga ccccggctga cacctcgcaa tgcagtcggt
      481 tttttcgggt ctggcagtat ggcgagtcgc cccgcctaac tgaccactca gcttacttca
      541 ggctaactac gtttaagatg tcagcccttt tctgccgtcg acgagaccaa tcctaatagg
      601 gaaatgtgag ttgtcaacgc ccgggattgg tgcaatcgac agatattttg ggccgctagg
      661 cgttccgtgc aagctattga agacaagcgg cttgcaattc tcgtcaacgg gaatcgcgga
      721 agttcttcac attgttttca cagaacttga tggcagaaag acacaaggca tccggaaacg
      781 ccacagtgcg gcgcggctga ggccctcagc cgacaggaac cgttcatcaa ccagaatctc
      841 gcctaccagg cgttgggcat gctcacacaa ctgctgcgcc atggctctgt ttcgtatcaa
      901 agcggtctct gcaaccttga gaccggtcag ttcgtgccgt tatcgattcg cggctgtcga
      961 attaagacct aggcacagct agtgactctc cagtaacgaa gtgaggttat cctcctgccg
     1021 tcattccaga gatcttgccg acttccaatg cgcgaacccc tacaattggg gtggcgcatt
     1081 gagtgtggct atggcaattt actcgaccgt ttgcggtcgc accgttggcg ctcagctgca
     1141 tggtgaggtg atctagtgaa cggcatcttg gtgcgcatcg gggtagatca agcgtacggg
     1201 aaatggaatg cgcctgttga cgaggcaacg cacgaatttg tcttcgtccc aattcctgac
     1261 ggcgatggga agcaatatag acctggatta cgagtgccat acgacaatat tcgcgccgac
     1321 gtgaggatct ttgcccaaca aaagaatttt ccggcagact gggagtttgg atttcccgag
     1381 gcgttgcgtc agcatagcgc ccatctcgat cccgacttcc gctggctgac gtacggtgac
     1441 aacggcgccg tcagaggcaa gggcataagg cagttgaagc gcggtgacct gctcgttttc
     1501 tactccggcc tgcggtcgat ccaacgacaa aacgtgctcg tttacgccat tactggattg
     1561 tacgtggtag acgaggtcgc acgcgccgcg gaaatcccag ccgaacggca tcgcgagaac
     1621 gcacacacaa gatgggatgt catcagtccg aacgatatcg tggtgatcgc acgtgctgac
     1681 gtctctggaa gacttcaaag gtgcataccc atgggcgaat ttcgcgatcg agcttaccgt
     1741 gtcacccccg atttggaagc agcgtgggga gggctttcgg ttaagaatgg ctacgtgcaa
     1801 cgcagtgttg taccgccgag ttttcgtgat gccggtcgat tctatgactg gtttctgcaa
     1861 caggacgttt cgttagtgaa gaggaacaac taggtgccag agtgccacgt cttgatcgtc
     1921 catttgcgtc ggccagaccg ttcgagaccc gacgagaaac ggaccgaccc gttctgggag
     1981 ttcggcagct ttggcatcac aacatgccac gcgaagaact tactgaagcc gggcaacgtg
     2041 aagcgactga aaggggttcg ctttgcattt gcccaaggtg gcaagaatga aatgaaactg
     2101 gtgttgctca cgcctccgat cagaagaata gttgaataca aagaccgatc agaagtgcta
     2161 tgggatcggc gtttcccatt caaattcagg aatgcaccgc tgctcatcga cagtgacggc
     2221 aaaagtgact ttccatcgct caagaaccat ctgaagggcg tgcggcgtag cacatgggtg
     2281 gcgcggttct caagcaagtt tcgttcgcga actacctatc tcgacccgga atttgcgaac
     2341 gagatcatgg ctgtattcga cgccaaatat aagaaggcaa ctcgagagga cctcgcaacg
     2401 ttgtacgaag atgcgctgcc ctaccctcca cattgcatcg acacgaatcg cgagaaaact
     2461 tacgaaagat gccagaaagg ggcggtgcgg aaaggcagtg gctgcggggt gaggcgccgg
     2521 ctttgaagaa catcaagaag tgcgccggcc acgctggttg ctgattggta agactgggtg
     2581 cgatgttaac agctctgaca aatttaaagg tcggcgcgtg tctcagatga atcgagctcc
     2641 gtctggaaaa cccgactgct acaattttgg gcgcaccatt tgaaaggact ccatggatat
     2701 ccaaaccata gtctcagatc tcaaggctga acgtaatcgg attgaccagg tcatctccgc
     2761 acttgaagga cttacttcaa ctgcactccg gcgtggacgt ccgccaaagg cggcaacgac
     2821 tggcgggaat cgtcgccgga tgagcccagc cgcacgcaag cgcatctccg aggctatgaa
     2881 gcagcgatgg gcaaaatgga agggaaagtc cgcaccgaag caagacaaga ctacgcctaa
     2941 gaagaccacc gtacgccgtc cgatgagcgc agccgcgaag aagaagttgt cagcactcat
     3001 gaaggcgcgt tgggccgcga agaaagaggc gaaagcataa gtgtcttcct catccttcgg
     3061 aaaacacgcg agtggcgcca caagtggtca atctgcagtt cccgagcctt cgttcgccga
     3121 acgagcgcgc acgttgatgc actcgagccg catcgggagt ctctcgaccc tgtcacggaa
     3181 acaacccggt ttcccattcg gatcattgat gccgtatgcg cttgacggcc aagggcgccc
     3241 aatttttctg atcagcacga tggccatgca tacgcagaat cttctggcgg atccgcgcgc
     3301 cagcctgttt atgacagagc ccgaggccag cggtgatccg ctcggagcgt ccagagccac
     3361 gttgatcggg agtgtcagca gaataccgga gccagatctg gcagccgcgc gtacggtcta
     3421 tctgacgcgt taccctgata gcaagcactg ggtcgatttt gacgatttct tcttttaccg
     3481 catggacgtt ttggacgttt attacgtggg c
//
LOCUS       DASRKK010000779         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1453951, whole genome shotgun sequence.
ACCESSION   DASRKK010000779 DASRKK010000000
VERSION     DASRKK010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453951"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFC15_18735"
     CDS             complement(<1..234)
                     /locus_tag="VFC15_18735"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HZM12249.1"
                     /translation="MKREVALKIVLESIGLLFLALAYPLIMFVRQDPGTSMMFSIYVT
                     LGVFLLLAIRNPLASRSLIAFTAWSSFAHAAVMG"
     gene            complement(531..1997)
                     /locus_tag="VFC15_18740"
     CDS             complement(531..1997)
                     /locus_tag="VFC15_18740"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM12250.1"
                     /translation="MPKLQRIAIAGATGYIGGRLVPQLLASGYAVRCLVRSPAKLEGR
                     AWALDSRVEIRRTDLADAPSLTRELETCGAAFYLVHSMMSAGDEYAQRDRQLALAFGR
                     AAHDARVGRVIYLGGLGETGAHLSKHLSSRRDVEGALASAGVPVTVLRAAMIIGSGSA
                     SFEILRYLVHRLPVMITPKWVSTPCQPIAVQNVLTYLVGVLAVPETTGGVFDIGGPEA
                     LCYSDIIRLMAEELGLSRRWLIPVPVLTPRLSSYWIHLVTPLSHKIAIPLAEGLKNPV
                     VCRDDRITRLVPQKLLNVREAIRAALSQVAARQVETNWSMAGPMPGDPDWSGGTVFRD
                     TREVAIDAPAGAVFRAVCRLGGQRGWHGANWLWIIRGWLDRLAGGPGLRRGRRDPEAL
                     SYGEALDFWRVVGLEPDRSVSLRAEMRLPGEALLDFRIEPRNKGQCILHQTALFRPRG
                     LLGLIYWYAVLPFHHLVFRGMLAGIQRDAVQVALLPQT"
BASE COUNT          516 a          730 c          725 g          443 t
ORIGIN      
        1 gcccataacg gcggcatgag cgaagctcga ccatgcggta aaagcgatca ggctgcgact
       61 cgcgagtggg ttgcggattg cgagaagcag gaaaacgccg agcgtgacat aaatgctgaa
      121 catcatcgac gtcccagggt cttgccgcac aaacatgatc aagggataag ctaacgccaa
      181 aaagagcaat cctatcgact ccagcacaat cttaagagcc acttctcgtt tcatttatgc
      241 cctcccgcat atttgtttcg caacacccgc cgttccgcac ccggaattat caggcattca
      301 cagaattcga acgaaccggc ccggcgagcg cttgccgttc tggggcgctg cgctctcgcg
      361 aatcccgtgc tgactcatta tcacgcaaaa cgcgctatcc gcggatgtgg cggcccgatt
      421 ccgagtggat cgtcgggtat ccggaagtta tcgcgaaata ccctttggtt gaagtagggg
      481 atctcgcaac agaagtgcag ggttccgccg tgagcggtgt tgggcgaggg tcacgtctga
      541 ggcagcagag cgacctgaac ggcgtcgcgc tggatgccag ccagcatccc gcggaacaca
      601 aggtgatgga acgggagcac cgcgtaccaa tagatcaaac caagcagccc gcggggtcgg
      661 aacaaagctg tctggtggag gatgcattgg cctttgttac gtggttctat tcggaagtcg
      721 agcaacgctt cacccggtaa tcgcatttcg gcgcgtaacg agacgctacg gtctggctcc
      781 aagccgacga cgcgccagaa gtcgagcgcc tcgccgtagc tgagggcctc gggatcacgg
      841 cggccgcggc gcaatccagg ccctccagcc agccgatcca gccagccacg aatgatccag
      901 agccaattgg cgccatgcca gccacgttgc ccgcctaagc gacatacggc gcggaatact
      961 gccccggccg gcgcgtcgat ggccacttcg cgggtgtcgc gaaagactgt gccaccagac
     1021 caatccggat caccgggcat cgggccagcc atcgaccagt tcgtctccac ctggcgcgcc
     1081 gcaacttggc tgagtgcggc gcggatggcc tcgcgaacgt tcaacagttt ctgcgggacc
     1141 aaacgagtga tccggtcgtc acggcacacg actgggttct ttagtccttc ggccagtggt
     1201 attgcaatct tgtggctcaa tggcgtcaca agatgaatcc aataagaact caagcgaggc
     1261 gtcaggactg gaaccggtat gagccagcgt cgggatagac cgagctcttc cgccatgagg
     1321 cgaatgatgt cgctgtagca cagcgcttca ggcccaccga tgtcgaaaac accaccggtg
     1381 gtttctggga ctgctagcac gccgaccagg tacgtgagca cgttttggac cgcgattggc
     1441 tggcacggag tgctcaccca cttcggtgtg atcatgactg gcaaccggtg gacgaggtac
     1501 cgcagaatct cgaaagacgc cgagccggag ccgatgatca tagcggcacg aagcaccgtg
     1561 accggcactc ccgcagaggc aagcgccccc tccacgtcac ggcgcgaaga cagatgcttg
     1621 cttagatgcg caccggtttc acccaggccg ccaaggtaaa ttacgcgccc cactcgggcg
     1681 tcgtgggcag cccgtccgaa tgctagggcg agttgccggt cgcgttgagc atactcgtcg
     1741 ccggccgaca tcatcgaatg caccaggtaa aacgccgcgc cacacgtttc caactcacgt
     1801 gtcagcgatg gcgcgtcggc gagatcggtt ctgcggatct caacgcggga gtctaaggcc
     1861 caagcgcggc cttccaactt cgcaggagag cgcacgaggc atcgcaccgc atagccggaa
     1921 gcgagcagtt gcggcacgag tctcccgccg atatatccgg tagcacccgc tatggcaatg
     1981 cgttgcagtt tgggaattcc ttgggccgtg agcctagctt cagtctatcg gaaaggcgct
     2041 ttgatttctg tgccagcccc ccgtcgagat ccgcggctgt cggcagaacg ccaatcagcc
     2101 caactgagac gatcctggcc gcgaagtcgg ctctagttgg gctggtagac cattcccggc
     2161 gatcacagcc cttccatccc ggtacagcgc ttgattcaat tgcagccgat aggcaacaat
     2221 tgaatcgttg aatgccgcca gtcgggtact cgctgcggca ctacgtcgcg gctaggggaa
     2281 agcgtccatt ttgcggagtg gggtactccc tgtcagatcg caatgccgcg gtacgagggc
     2341 agattcgcct ggagcggcta cgcttgggca atttcggcga tgccgatcct tgggcctgaa
     2401 ctgcgcatcg atat
//
LOCUS       DASRKK010000780         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_85403, whole genome shotgun sequence.
ACCESSION   DASRKK010000780 DASRKK010000000
VERSION     DASRKK010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85403"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1610
                     /locus_tag="VFC15_18745"
     CDS             108..1610
                     /locus_tag="VFC15_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM12251.1"
                     /translation="MSTAVSTAQMHSRVQEFVSRPRKMFIDGKWVEAASGKTFPTYNP
                     ATGEVLAQIAEGDREDIDRAVKAARKAFESGPWPEMTASQRGRLIWKLGDLIEEHLEE
                     FAQLESLDNGKPLSVARVADVPLAADLFRYMAGWATKIEGNTIPISASSKHVKYFAYT
                     RREPVGVVGQIIPWNFPLLMAAWKLGPALATGCTVVLKPAEQTPLSALLLAELIAEAG
                     FPDGVVNVVPGYGETAGAALASHPEVDKIAFTGSTEVGKLILHAAAGNLKKVSLELGG
                     KSPNLVFADADMKATIPGAAGAIFFNHGQCCCAGSRLYVEQSIFDHVVHGVAEQAKKI
                     KLGPGFDPETSMGPLVSEEQMNRVCSYLESGLHEGAEAVVGGHKRPGAGYFVEPTVMV
                     KTKPDMKVVREEIFGPVVCAMPFRDVDKLIAEANQSEYGLAAAVWTRDISKAHRIAEK
                     LRAGTVWINCYNVFDAALPFGGYKQSGWGREMGHESLELYTEVKAVCAAI"
     gene            1786..2466
                     /locus_tag="VFC15_18750"
     CDS             1786..2466
                     /locus_tag="VFC15_18750"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HZM12252.1"
                     /translation="MNKAQTQEGFQTAVADFIDVCPHCSTKAHLKLLFAESYQANNRD
                     LIYYALFRCVPCKKLTLQTFRFSQNEYDPNENLSAVGWQQKFPGDEITYASKFEGTVP
                     REVLEDFAEGVISLHNKCPRAATAMFRRSLHSALLERGSNTKQDLVDQIKNASFLTQD
                     IKDWAHNIRIFGNWGAHPQDDNLREADEPIAKETQAFLEECFNYVYVMPNRVAKARAA
                     KQQKKDAE"
     gene            complement(2535..>3014)
                     /locus_tag="VFC15_18755"
     CDS             complement(2535..>3014)
                     /locus_tag="VFC15_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12253.1"
                     /translation="PSLFRICETTFQGWKRYHNDPDLRVRRRFAAEARKLRTTYNAAL
                     WAMEKRLSATNPTISKRIRELRREIESAFGTFTRMVRVVVGPILLWTSKREDKRLANG
                     ITYEPETFVERRNWIADKLRHTADVITMHPRHSEQPLTNIAAARVAVESFSEVTAGD"
BASE COUNT          664 a          806 c          910 g          634 t
ORIGIN      
        1 tcttgcgcgg catgtgggct cgacgtccat cgctccgtcg cgagcgtccc tgacatcacg
       61 gatagcgcgg aattctggcg gcggcactcg gcatgaggag gttgtttatg tctaccgcag
      121 taagtacggc gcagatgcac agtcgtgttc aggagttcgt cagcaggccg cgcaagatgt
      181 tcatcgatgg aaaatgggtg gaggcggcct ccgggaaaac cttccccacc tataaccccg
      241 ctactggaga agttttggcg cagattgccg aaggcgatcg cgaggacatt gatcgcgcgg
      301 tgaaagcggc gcgcaaggcc ttcgagagcg gcccctggcc tgagatgacg gcctcacagc
      361 gtggccgcct gatatggaag ctgggtgact tgatcgaaga gcacctggaa gaattcgcac
      421 agctcgaaag tttggataac ggcaagccgc tatcggtcgc gcgtgtcgcc gatgtgccgc
      481 tggcggccga tctgtttcgc tacatggcag gttgggccac caagatcgag ggcaacacga
      541 tcccgatttc ggcgtcgtcg aagcacgtga agtactttgc gtatacccgg cgcgagcccg
      601 tcggagtggt gggacagatt atcccctgga atttcccgct gttgatggcg gcgtggaagc
      661 ttggtccggc gctggcgaca ggctgcaccg tggtgttgaa gccggcagag cagactccgc
      721 tgagcgccct gttgttggcg gagctaattg cagaggcggg ttttccagac ggtgtggtga
      781 acgtggtgcc gggttatggc gagacggcag gagctgcgtt ggcgtcgcat cctgaagtgg
      841 ataaaatcgc cttcaccggt tccaccgaag tcggcaagtt gattttgcac gcggccgccg
      901 gcaatctgaa gaaggtttcc ctcgagctcg gcggcaagtc gcccaaccta gtcttcgcgg
      961 acgccgacat gaaggcgact attcctggag cggcgggtgc catcttcttc aaccacggtc
     1021 aatgctgctg cgcggggtcg cggctgtacg tcgagcagag catcttcgac cacgtggtgc
     1081 atggcgtggc cgagcaggcc aagaagatca aactaggacc cggtttcgat cccgaaacct
     1141 caatggggcc gctggtctcc gaggagcaga tgaatcgcgt ttgcagctat ctggagagcg
     1201 ggctgcacga aggcgccgaa gcggtggtgg gcggacacaa gcggccgggc gcgggatatt
     1261 ttgtagagcc gacagtgatg gtaaagacca agcccgacat gaaggtggtt cgcgaggaga
     1321 tctttgggcc ggtggtttgc gccatgcctt tccgcgatgt ggacaaactg attgccgaag
     1381 ccaaccagag cgaatacggc ctggcggcgg cggtgtggac ccgcgatatc agcaaggccc
     1441 accggattgc cgagaagctg cgcgccggta ccgtatggat caactgctac aacgtgttcg
     1501 acgcagccct gccgtttggc ggatacaaac aatcaggctg gggccgcgag atgggccacg
     1561 aatcgttgga gctctacacc gaagtcaaag cggtttgcgc cgcgatctag gctgccctaa
     1621 tcgccgcttg tcacctggct gggtgcccca ctcattcctg gttttggaat gagtgggaac
     1681 ggattacttc gatgtaggag ttttcgatag catcaactct gtggttccac acattcgaaa
     1741 gtcgcgaatg tgtggggcac ccggcgatag gtacaattac acacgatgaa taaagctcaa
     1801 acgcaagagg gtttccaaac ggcggtagcc gattttatcg atgtgtgtcc tcactgtagc
     1861 accaaggcgc atctcaagct tctgtttgcg gaaagttatc aagccaacaa tcgggaccta
     1921 atttattacg ctctctttcg ctgtgtaccg tgcaaaaaac taacactaca aacatttcgc
     1981 ttcagtcaaa acgaatacga tcccaacgaa aacctgtcag cggtaggttg gcagcaaaaa
     2041 tttccagggg acgaaataac atacgcgagc aaatttgagg gaaccgtgcc gcgcgaagta
     2101 ctcgaggatt tcgcggaggg agtcatttct ttgcataaca agtgcccccg cgccgcaaca
     2161 gcaatgtttc gccgatcttt acactcggcc ttactcgaac gcggatccaa caccaagcaa
     2221 gatctcgtcg accagattaa gaatgctagc ttcctaactc aagatattaa agactgggct
     2281 cataatatcc gcatatttgg caactggggt gctcatccgc aagacgataa cctccgagag
     2341 gccgatgagc caattgcgaa ggaaacgcag gcctttcttg aagagtgttt taattatgtg
     2401 tacgttatgc ccaatagagt tgccaaagct cgtgccgcta aacagcagaa gaaagatgcc
     2461 gagtaagtga acttgcgagg attcattgtg ctgtcccaca cattcgaaag tcgcgaatgt
     2521 gtggggcacc cgacctaatc gccggcggtc acttcactga agctctcgac cgcgacccgt
     2581 gcggccgcga tgtttgtcag cggctgctcg ctatggcgcg gatgcatggt gatgacgtcc
     2641 gcggtgtgcc gtagcttgtc ggctatccag ttgcgacgct cgacgaacgt ctccggctca
     2701 taggtgatgc cgttagccaa gcgtttgtct tcgcgcttcg aagtccataa caatatcggc
     2761 ccaaccacga cgcgtaccat gcgggtgaac gttccaaagg cgctttcgat ttcgcgccgc
     2821 agttcgcgaa tccgcttcga tatggtggga ttggtcgcac taagccgctt ctccatggcc
     2881 caaagtgccg cattgtaagt cgtgcgcaac ttcctggctt ccgcagcgaa gcgacggcgc
     2941 actcgcaggt cgggatcgtt gtgatagcgc ttccatccct ggaaggtagt ctcgcaaatg
     3001 cgaaacaagc tggg
//
LOCUS       DASRKK010000781         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_671731, whole genome shotgun sequence.
ACCESSION   DASRKK010000781 DASRKK010000000
VERSION     DASRKK010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671731"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="mutM"
                     /locus_tag="VFC15_18760"
     CDS             complement(<1..792)
                     /gene="mutM"
                     /locus_tag="VFC15_18760"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZM12254.1"
                     /translation="MPELPEVETIARGLNQRIAGDVIESVWLGEKPEPLKSPAAEIAS
                     VLEHRRIVQVRRVGKHIVFDLQRSRRNMSRRGAALDGQWIVHLGMTGRLLIASSEVAM
                     AKHTHAVLKLASGREVRFVDPRRFGRLAVVRIESKIATGFAAPGDEPLQSQIGPFMAL
                     FRGRKTPIKSALLNQKLLSGVGNIYADESLFRAGIRPRRRAASLTRAELQRLHIALKA
                     VLREAIKLGGSSVNDYVDAEGQEGLFQLRHRVYGREGEPCLVCGTA"
     gene            1401..>2090
                     /locus_tag="VFC15_18765"
     CDS             1401..>2090
                     /locus_tag="VFC15_18765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZM12255.1"
                     /translation="MRTGKTAVIVGAQWGDEGKGKIVDVLSERFNVVARYSGGHNAGH
                     TVTIKGKKFILQLIPCGVLREGCRGVIGNGVVLDPFAFLKEVDALRATGVHVDGNLFV
                     SNRAHVILPYHRMIELASENAPGRVKIGTTSRGIGPTYEDKMARSGLRVQDLLDKTLL
                     RKHIENACREKNTIAHALFNSEPLDPDKMFADYAEIADRVAPFVTDTALLLNRAIAEG
                     ESVMFEGAQGTM"
BASE COUNT          430 a          618 c          602 g          440 t
ORIGIN      
        1 ggctgtgccg cataccaggc atggctcgcc ctctcttccg taaacgcgat gtcgcagttg
       61 aaacaatccc tcctggcctt cggcatcgac gtaatcgttt acggacgatc ccccgagctt
      121 gatcgcctcg cgcaggaccg cttttagtgc gatgtgcagt ctttgcaact ctgcgcgcgt
      181 gagcgacgcc gctcgcctgc gtggccgaat cccggcgcgg aacagcgact cgtcagcata
      241 aatgttcccg acaccgctta acagcttctg gttcagcaat gcacttttga taggagtttt
      301 acggccgcga aatagagcca tgaatggccc gatttgcgac tgcagcggct catcgccggg
      361 cgcggcgaat ccggtggcaa tttttgattc gatccgcacg acggccaagc ggccgaatcg
      421 ccgaggatcg acgaaccgca cttcccttcc cgaggccagc ttaagcaccg cgtgggtgtg
      481 cttggccatt gcgacttccg aggaggcgat tagcagacgg ccagtcattc cgaggtgcac
      541 aatccattgc ccatcaaggg cagcccccct tctcgacatg tttcgtcggg aacgctgcaa
      601 atcgaaaacg atgtgcttgc ccacgcgccg tacttgcacg attcggcggt gctccagaac
      661 ggacgcgatt tccgcggcag gcgatttcag gggctcaggt ttttctccca gccagaccga
      721 ctcaatcacg tccccagcga tccgctgatt gagtccgcga gcaatggttt cgacttccgg
      781 aagttcgggc ataaactgtt agtgtacggc ggcatcctct ccttgagtga gacccatgaa
      841 gtttagagtt ggcatcgcgg ctgccatcgg ctttctcgtg ccggtgtttc ttggatcgcc
      901 gcggtgctat tgtttagccc cagcctggat tattcatctg tttcggtagc taaagacagc
      961 aagctttgaa agggcacgcc ttcagccgtg ccgttacctg tgcgtggaga gttgggcttc
     1021 agtccctgag ggaactcgca cttcgtctca acaccgaagt ccctcagcgg ctaaagccac
     1081 cgtaatgaca gcgcttacgg cacggctgaa gccgtgcccc ttcaaagcgt gaagtcctca
     1141 tgaataatcc agcccagggc aagcaattct tggatcacca tctgtggctc cgagtgagga
     1201 aggcctattc caactgaacc cattgagtgc acacacattg cgccggtcag actggaacct
     1261 gtaaaattca tagttacacc gcccgtctca cagatacttc cccgaatttt ggacttgctg
     1321 tgcaatccag aggcgagttg tgtgcgaatc tgcgcggtcg ttcgcgctcg ataccgggcg
     1381 tccgcgaatg gagcacgaag ttgagaactg gcaagacagc agtgattgtc ggcgcgcagt
     1441 ggggcgacga aggtaaaggc aagatcgttg acgttctctc cgagcgcttc aacgtcgtag
     1501 cgcgctattc tggcggccat aatgcgggac acacggtcac gatcaagggc aagaagttca
     1561 ttcttcagct catcccatgc ggagtgctgc gtgagggctg ccgcggcgta atcgggaatg
     1621 gggttgtgct cgatcccttc gccttcctga aggaagtgga tgcgctgcgc gccacgggcg
     1681 tgcacgtcga cggcaatcta tttgtcagca accgcgcgca tgtgatcctt ccctaccacc
     1741 gcatgattga actggcatcg gagaacgctc ccggccgcgt aaagattggc accacgtcgc
     1801 ggggtattgg gccgacctac gaagacaaga tggcgcgcag cggattgcga gtgcaagacc
     1861 tgctcgacaa gaccctgctg cggaagcaca ttgagaatgc ctgccgcgag aagaacacca
     1921 tcgcgcatgc gctgtttaac agcgagcctc tggatcccga caagatgttc gccgactacg
     1981 cggaaatcgc agaccgcgtc gcccccttcg tgaccgacac cgcgctgctg ctcaatcggg
     2041 ccatcgccga gggcgagtcg gtgatgttcg aaggggcgca aggtaccatg
//
LOCUS       DASRKK010000782         9861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1258260, whole genome shotgun sequence.
ACCESSION   DASRKK010000782 DASRKK010000000
VERSION     DASRKK010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258260"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1196)
                     /locus_tag="VFC15_18770"
     CDS             complement(183..1196)
                     /locus_tag="VFC15_18770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM12256.1"
                     /translation="MTNSKPLRFAILGTSGHANRIAAPILKQNPGVSLVGAAGSAPGR
                     GTRFAEQHGLSRGYESLEELLADPQVDAVWLCSPNHMHARQVAQCAAAGKHILVEKPL
                     ATTRRDADAAAQAVAKAKVTLRVGCQHRFRPSHGHLRELVRSGVVGQIGYFRIHRFWR
                     YPYYEDMGPPNWRQSPTESGGWVINDLGSHLLDLMLWISGAEGQTAGAILASQKFAAS
                     TEDSTAVLVKLDKAGIGIMETSCANDSPGSRIEIYGNAGWIRAENTLTGAATILTHDG
                     QSLPFAPVEVMDIYCDEVRDFVGAVRGQLSIGADAMAGAAVAGLIESALATGVRARAV
                     PAG"
     gene            complement(1423..2379)
                     /locus_tag="VFC15_18775"
     CDS             complement(1423..2379)
                     /locus_tag="VFC15_18775"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM12257.1"
                     /translation="MVEQRIAVIGMGQMGSAMAERLGEVGLDVLGFDINIQTRQRLAG
                     RGVPVSDSLRAALAGRNVILTSLPDPKAVRAVWLGEGGIVALAEPGNLCIELSTIDPA
                     TMREVAEAGTQRKLAVVDCPVSGSPSEARQGKLILIVGGERSVVDRARSLLLHLGETC
                     RYTGDIGTAKVVKIVNNMMSMGNVLVASEAFALGVAAGVEPQTLYDALSVSGGRSHHF
                     TKRFPNALKGDFSPRFKMELGEKDLALAIELGRSVKLPTPTASIVRELFALALTEGFR
                     GAGYRGARRYVPALDQARWKGTEGRKVVIGAAPSLRAQQAVH"
     gene            2956..3420
                     /locus_tag="VFC15_18780"
     CDS             2956..3420
                     /locus_tag="VFC15_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZM12258.1"
                     /translation="MNPSHRLTELGLSLPTPARPTFKYVPVVVHGGLAFVSGQLPWVD
                     GEIKTVGLLGATISLEQGKEAARICGLQALACLEQALGSIDQIVRIIKLTGFVQSTSD
                     FHQQPSVIDAASELMFDIFGEEGRHARSAIGVAALPRNAPVELEVIVAVRTR"
     gene            3463..4128
                     /locus_tag="VFC15_18785"
     CDS             3463..4128
                     /locus_tag="VFC15_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12259.1"
                     /translation="MAKAPFRLRMYHDHLVEGGEATLAAERRIIYCVAGDAEISSEGR
                     NVKLCEDEGYFFNQKVVVRGGSEGACLWRWELVQPNTAAGEIQRQGIKSTNAGDYKIE
                     LDTSVKRLMRLDRVDFPLGGEALTHIHAAPGVRCQLTGSLLLDALGKRHRVWPGDTWV
                     EHGPDQVYAKASEMHLSSFVRVMIVPEEYKGRSTITYIRPEDKDKPKSQSYKRYLEEP
                     ISF"
     gene            4146..5018
                     /locus_tag="VFC15_18790"
     CDS             4146..5018
                     /locus_tag="VFC15_18790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZM12260.1"
                     /translation="MNLQTLIFQLLNGLVWGLILALITLGLTLIFGILSIINIAHAAL
                     YMLGAVLAWEVIQYTGSFWAAMVIAPLLVGLVSVAIERVVLRPISGNFVLSILATLGM
                     LFIIQDTMLATFGGGLQRIANPIRGTMSIFGLHYPYYRLFVAGVSISTMLVVWVFLNR
                     TKYGLWIRAVKQDPQIATAMGIPVSIVYMSTVALGGILAGLSGVLAGPIVAIEYQMGL
                     KILAYSFIIVVVGGFGSIMGSIVVAIVIGCLDGVFTTYFPASEARIMTLVLVSLILLL
                     RPEGIFRARRETVI"
     gene            5019..6032
                     /locus_tag="VFC15_18795"
     CDS             5019..6032
                     /locus_tag="VFC15_18795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZM12261.1"
                     /translation="MRYRRQILWLFAGALLFAALPVVFGNYGLYLGSALCVWIIFALA
                     YDLAFGRAGLLSFGHAMFFGVGAYALALLVLTLDWGFWPAITAAIVLAGAFALVLGKV
                     ALQVTGHAFVVITVIFSAILELVAFSQKWITNGEDGLSFDLYRVPFGFAELDLRNANT
                     KYWVLIAALFLTFLMLNWINNSTLGLAFRAVRENPMRATLLGYPVQHYKLAAFGISGA
                     LSGLAGALFSLLSFHISAEVFQITVSINPLTSVLVGGAGTMLGPVVGTTFIFLFSDYL
                     RTLVVYADFAIGVILVLTVLFAPQGIVGLIERIKARRRARTDNKAEASLSPNDESIAE
                     TRS"
     gene            6029..6808
                     /locus_tag="VFC15_18800"
     CDS             6029..6808
                     /locus_tag="VFC15_18800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM12262.1"
                     /translation="MTAQVGSVILEASGIAKYFDGLKALTDVNLKVFRGECLAIIGPN
                     GAGKTTFFNILTGLLRPTAGRILFEGADITTYAPHRRAAIGISRTMQITSVFPRLSVL
                     DNVLIGIQGQHAPWKILRLGSLPMDEMRRRAAEIVELVGLGEKADVICANLAHGDQRL
                     VEIALALSVEAKLLLLDEPTAGLSLNETRHITGRLREIWERKKLTIVIVEHDMEVALG
                     LATRVAVFHQGTCLAIGEPDVIMADEVVNRVYLKGHARAEA"
     gene            6789..7505
                     /locus_tag="VFC15_18805"
     CDS             6789..7505
                     /locus_tag="VFC15_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM12263.1"
                     /translation="MPVLRLEKVNAGYGDSHVLHDVDLDFGENRIIALLGRNGVGKTT
                     TFNAIMGLVPVKSGRILFDGHDLTCLPAQSAALLGIGFVPQGRRIFPEFTVMENLRVG
                     TRTGRVPLDTLDWIFSLFPRLKERLVQVGGSLSGGEQQMLAVGRALAMQPRLILMDEP
                     MEGLSPGMAEVVRHAIVEARDKGMRILIIEHDIDEALALAQDIHIMEKGSIRVHCRPQ
                     LLIQDPAILERHLGIRLHAV"
     gene            7554..8846
                     /locus_tag="VFC15_18810"
     CDS             7554..8846
                     /locus_tag="VFC15_18810"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM12264.1"
                     /translation="MIKKNPDTTANMRKVVGTDMTRRRLVLASGVAMAAAVVGAPTVL
                     RAANKPIVIGHQLDQTGILAVYAPWYDRAAKGAIDRINKAGGIDGRQVKYVVENTASK
                     VDIGLAAIEKLVKREESDFILGALHSGIALGSTKVCKSLNTLYFSAAHAEEVTGSGGN
                     RFVFRVSSQSESEGEATATKELTSELGSKWSIFYADYAWGQSHARVWRERLKRNGAEI
                     LSEVAMPLGASDLVPYLSKIHRDTQACVVALFASDAVGFIQQREALGFNFKLMGMQGL
                     AAGIPPDVLKLAAGFYVPESLPRRLKDKDTPHNKLLRQTVLADENGKEIGGNQWLTES
                     YYWVAWTHVNLIKKAVEASGWKSKADNLKMIEAMEGMKLEESDDFPQGSTTIRAADHQ
                     AFVDLHLTQIQSDGFAKVIRKLAPKQYPAPIDYRKETL"
     gene            complement(8995..9645)
                     /locus_tag="VFC15_18815"
     CDS             complement(8995..9645)
                     /locus_tag="VFC15_18815"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZM12265.1"
                     /translation="MYNTSRIFSDSWAFLGRWDIAAERLVGCDVWPLVALGDIFNIAR
                     GGSPRPILDYVTDSPDGVNWIMISDASEGSKYITKTKKRIKPSGVKRSRMVHPGDFLL
                     SNSMSFGHPYILKTSGCIHDGWLVLSAKKKNISQDYFYYLLRSPLMFLTFKGLAGGST
                     VKNLNIELVTGVKVTLPTLDEQKGYGTAPEAIPSQRLLQKSPVRAGGVTHRAHPRD"
BASE COUNT         2050 a         2887 c         2834 g         2090 t
ORIGIN      
        1 gatcagcgac taatattccg aacgcttgca ggatcgcaaa catcacttct aataatgcga
       61 tgattctgcc ttacaatgcg aatccacgcc ggatataatt ttcggaaagg acagcggcaa
      121 cgtacgcgtg ctctcgggaa gatcgctatc aattatgcaa gtctgcatac gcgtcgctgc
      181 cctcagcctg cgggaacggc acgcgcccgt accccggtcg ctagggcaga ttcgatcagg
      241 cccgcgaccg cagccccggc catcgcatct gcgccgatgc taagttgtcc acggaccgcg
      301 ccgacgaaat ctcttacctc atcacaataa atgtccatga cctcgaccgg cgcaaacggg
      361 agcgactgtc catcatgggt cagaatggta gctgcgccgg tcaacgtgtt ctcagccctg
      421 atccaacctg cgttgccata gatctcgatc cggctgccgg gactatcgtt ggcgcagctc
      481 gtctccatta tcccaattcc ggccttatcg agtttgacca aaaccgccgt gctgtcctcg
      541 gtcgacgccg caaatttctg cgacgcaagg atcgcgcctg ccgtttgtcc ttccgcgccg
      601 ctgatccaca gcatcagatc caggagatgg gaaccgaggt cgttaatgac ccagccgcca
      661 ctttcggtcg gagactgccg ccaattgggt gggcccatgt cctcgtaata gggatagcgc
      721 caaaatcggt gaatgcggaa atagccgatt tgtccgacca caccggagcg gacgagttcg
      781 cggagatggc catgtgaggg acggaagcga tgctggcagc caacgcgcag cgtgactttg
      841 gccttggcca ccgcctgcgc ggcggcatcg gcgtcgcgtc gcgtggtcgc gagcggcttc
      901 tcgaccagga tatgtttgcc ggccgccgca cactgcgcca cttgccgcgc atgcatatgg
      961 tttggcgagc agagccagac ggcgtcgacc tggggatcgg cgagcagttc ttcaagcgac
     1021 tcgtagccgc gcgacagccc gtgctgctcg gcgaaccgcg tgcccctgcc aggggcgctc
     1081 ccggcggcgc cgacgagcga aacaccggga ttttgtttga gaatgggcgc ggcaatccgg
     1141 tttgcgtgac ccgaggtgcc gagaatagcg aatctgagag gcttggaatt ggtcatgact
     1201 gctcgcttcg tttccaactt aaaaggtcga tgcatcgact cagatgatcg actcctagcc
     1261 gggttcttcc ttgcctctga caacttcgag gttgaatgtg actgcccccg cggccacgat
     1321 cagtcgcggt atccacagag gtaggtcgcc gggccctcac cggccgagca cggaaacgcc
     1381 ccggtccctg ctcgtcccag ccgcgtcagc accaacgacc gcctagtgca ctgcttgttg
     1441 cgcacgcagc gatggtgccg cgccgatcac gacttttcgt ccttcggtgc ctttccatct
     1501 tgcttggtcc agcgctggta catagcgacg agcgccacga tatcctgccc cccggaatcc
     1561 ttcagtgagg gcgagcgcaa aaagttccct tacgatcgag gcagtcggtg tgggcagctt
     1621 gacagatcgc ccgagctcaa tcgcaagagc gaggtccttc tcgcctagct ccatcttgaa
     1681 tctgggactg aaatcgccct tcagcgcatt ggggaaccgc ttagtaaagt ggtgcgatcg
     1741 gccgccgctt accgacaggg cgtcatagag agtctggggc tcgacgcccg ccgcaacgcc
     1801 aagcgcgaat gcctccgagg caacgagaac gttgcccatc gacatcatgt tgttcacgat
     1861 cttgacgacc ttcgccgtcc cgatgtcgcc ggtataacgg catgtctcgc caaggtgtag
     1921 aagcaacgat ctggcgcgat ccacgacgct gcgttcgccg ccgacgataa ggatcaactt
     1981 cccctgccgc gcctcactcg gactaccgct aaccgggcaa tcgacgactg ccagcttccg
     2041 ctgagttccc gcttcagcca cctcgcgcat cgtcgcgggg tcgatcgtgc tcagttcgat
     2101 gcagaggttg cccggctcgg cgagagcgac aattcctcct tcgccaagcc aaacggcgcg
     2161 caccgccttg ggatcgggca gactcgtcaa gatcacgttc cgacctgcca aggctgcccg
     2221 caggctgtcc gagacaggga cgccgcggcc agccaggcgc tgtcgggtct gtatgttgat
     2281 atcgaaaccg agcacatcga gaccgacctc acccagcctc tcggccatag ctgagcccat
     2341 ctgtcccata ccgataactg cgattcgttg ctccaccatc ggctcctttc gcctgtcggt
     2401 tccgaaaact ccatccattg ggtctcgatc gctggcaaat cagaacatga ttgctggatg
     2461 ttcgcaattg ccttcctttt tgtccgcgtt ctgtgcgact tgtgtgtacc ggaaacttaa
     2521 acccgaacgt aatggtgatg aagtccgcca aggatcgcat gtgaattgat agatccggtt
     2581 cgctgaaccg ggtgagagaa gcggcgcatc cttgtccaat gaccggcgtg ttctgatgtc
     2641 gttgtaggtg ggagcgtagg aatgcgagac ccggtgcaga tgcgatgttc cgtaacctgt
     2701 cattatgcga atccgaagca tagaaaataa gagatgcagt aatctcattt ccgctgatac
     2761 aaaaaaggcg gcaggtcaca gccgatcctg gcaaaatcct gcttcttgat acaaatcaat
     2821 gccacccccg tactgggctg gaattcttag agcgaaatcg ccgtcaacac caagggccgc
     2881 gccggcttca cgcttgaacg ggtcatcagc acgacaactg gcgtgccgaa gaatggaaat
     2941 gaaggaacgt tggcgatgaa tccgtcccat cggctcactg aactcgggtt aagcctaccg
     3001 acgccggccc gacctacctt caaatatgtc ccagtagttg ttcatggagg actagcgttt
     3061 gtcagcggac agctcccttg ggtcgatggc gagatcaaaa cggtcggatt gctcggcgcc
     3121 acgatttccc ttgagcaggg aaaggaagcg gcgcgtatct gcggcttgca ggcgctcgca
     3181 tgccttgagc aagcgcttgg ctctatcgac caaattgtgc ggattataaa actgaccggt
     3241 ttcgtccaga gtacatcaga ctttcaccaa cagccaagcg ttatcgatgc ggccagtgag
     3301 ctcatgtttg atattttcgg cgaagagggg cggcacgcca ggtcggcgat tggcgtcgcg
     3361 gccttgccaa ggaacgctcc tgtggaattg gaagtgatcg tggcagttcg tacccgctga
     3421 acttgaaaga aatcaattag caacagtgaa ataaggatga caatggcaaa ggcaccgttt
     3481 cggttgcgaa tgtatcatga ccacctcgta gaaggtggag aggcgacgct ggcggccgaa
     3541 cgtcgcatta tctactgcgt tgctggggac gctgaaatct cgagcgaagg gcgtaacgta
     3601 aagctctgcg aagatgaggg ctattttttc aaccagaaag tggttgtacg aggaggatcg
     3661 gaaggtgcgt gtctttggcg ttgggaactg gtgcaaccaa atacagccgc tggagaaatt
     3721 cagcgtcagg ggattaagag cacgaacgcc ggtgactaca agatcgagct cgatacctca
     3781 gttaaacgtt taatgcggtt ggatcgtgtg gatttcccgc tcggtggcga ggcattgacg
     3841 cacattcacg ctgctccagg cgttcgttgt cagcttaccg gaagtctatt gctcgatgcg
     3901 ctcggcaagc gccaccgcgt gtggccaggc gacacatggg ttgagcatgg accggatcaa
     3961 gtctacgcga aggcgtccga aatgcatctt tcaagtttcg ttcgcgtgat gatcgtccca
     4021 gaggaataca aggggagatc gacgatcact tatattcgac ctgaggacaa agacaaacca
     4081 aagtctcagt cttacaaacg ttatctggaa gaaccgatta gcttttaggt ttcgccggcg
     4141 accgaatgaa cctgcaaacc ttgatcttcc agctgctgaa cgggctagtc tggggcctga
     4201 tcctggcgct catcacgctg ggcctgacgc taatcttcgg cattttaagc atcatcaaca
     4261 tcgctcatgc cgcactctac atgctaggcg ccgttctggc ctgggaggtc atccaatata
     4321 ccggcagctt ctgggccgcc atggtaatcg cgcccctgct cgtcggcttg gtcagcgtcg
     4381 cgatcgagag ggtcgtactt cgcccaatca gcggcaactt cgtcttgagc atccttgcca
     4441 cgctcgggat gctcttcatc atccaggata cgatgctcgc gaccttcggc ggcgggctgc
     4501 agcgcatcgc caaccccatc cgcggtacga tgtccatctt cggcttgcac tatccctatt
     4561 atcggctgtt tgttgccggc gtctcgatct cgaccatgct cgtcgtctgg gtattcttga
     4621 accgcaccaa atacggactg tggatacggg ccgtgaagca ggacccgcag atcgccaccg
     4681 cgatgggaat accggtctcg attgtctaca tgtcgacggt cgcgctcggt ggcattctcg
     4741 ccggcttgag tggggtgctg gccggaccga tcgtggccat cgagtaccag atgggcctca
     4801 agatcctagc gtactccttt atcatcgtcg tcgtcggcgg cttcgggagc atcatgggat
     4861 caatcgtcgt cgccatcgtc atcggctgct tagacggcgt cttcacgacg tattttcccg
     4921 cgagcgaagc gcggatcatg acgctcgtgc tagtatcgct gatcctgctc ttgcggcccg
     4981 aggggatctt ccgggcgcgt cgggaaacgg tgatctaagt gcgctatcga cgacagatcc
     5041 tgtggctttt cgcaggcgcg cttctctttg ccgcacttcc cgtcgtcttc ggcaactatg
     5101 gtctgtacct cggctcggcg ctctgtgtct ggatcatctt cgcactggcc tatgacctcg
     5161 cgtttggccg agccgggctc ttgagcttcg gccatgccat gttcttcggg gtgggcgcct
     5221 acgcgttggc cttgctcgtc ttaaccctgg attggggatt ttggccggcg atcacggccg
     5281 cgatcgttct ggccggggcg ttcgcgctcg tcctcggcaa agtcgcactc caggtgaccg
     5341 gacacgcttt cgtcgtgatc acggtcatct tcagcgctat cctggaactg gtggccttct
     5401 ctcagaagtg gatcacgaat ggcgaggatg gcctgagctt cgatctttat cgcgtcccgt
     5461 tcggcttcgc cgagctcgat ctccgcaatg cgaatacgaa atactgggtc ctgatcgccg
     5521 ccctgttcct gacgttcctg atgcttaatt ggatcaacaa ctcgaccctc ggcctcgcct
     5581 tccgcgcggt ccgagaaaac ccgatgaggg caacgctcct tggctacccc gttcaacatt
     5641 acaagctggc tgctttcggc atttccggcg cgctgagcgg gctggcggga gcgctgttca
     5701 gccttctcag cttccacatc tcggccgaag tattccagat caccgtgtcg atcaacccat
     5761 tgacctctgt tctggtggga ggagccggga cgatgcttgg cccggtcgtc gggacaacgt
     5821 tcatcttcct cttctccgat tatcttagga cccttgttgt ctacgccgac ttcgcgatcg
     5881 gggtcatcct ggtgctcacc gtcctgttcg cgccgcaggg catcgtcggg ctgatcgagc
     5941 ggatcaaagc gcggcgtcgc gctcgaacgg acaacaaggc tgaggccagt ctcagcccca
     6001 acgatgagag catcgcggag acgcgttcat gaccgcgcag gtcgggagcg tgatcctcga
     6061 ggcgagcggc atcgccaagt atttcgatgg gctgaaggcg ctcacggacg tgaatctcaa
     6121 ggtgtttcgt ggtgagtgtc tcgccatcat tggaccgaat ggcgcgggta agacgacgtt
     6181 cttcaacatc ctgaccggac ttctgcgacc gaccgccggc cgcattctgt ttgagggcgc
     6241 cgacattacg acctatgcgc cgcacagaag ggccgcgatt ggcatctccc ggacgatgca
     6301 gataaccagc gtctttcctc gtctgtccgt gctcgacaac gtcctgatcg ggatccaggg
     6361 gcagcacgct ccatggaaga ttctgcgcct cgggtccttg ccgatggatg agatgcggcg
     6421 gcgggcggcc gagatcgttg agcttgtggg ccttggcgaa aaagccgatg tgatctgcgc
     6481 caacctggcg cacggcgacc agcggctcgt cgagattgcg ctggcattaa gtgtcgaggc
     6541 gaagcttctc ctgctggacg agcccacggc ggggctcagc ctaaatgaaa cgcgtcacat
     6601 cacgggccgc cttcgcgaga tctgggagcg caagaaactc acgatcgtaa tcgtcgagca
     6661 cgacatggaa gtggcactcg gtcttgctac ccgcgtggca gtcttccacc agggcacttg
     6721 cctcgcgatc ggcgagccag acgtgatcat ggccgacgag gtcgtcaatc gcgtctattt
     6781 gaaagggcat gcccgtgctg aggcttgaga aagtcaacgc aggatacggt gacagccatg
     6841 tgctgcacga cgtcgacctc gattttgggg agaaccggat catcgcgctg ctcgggcgca
     6901 acggcgtcgg gaagaccacg acgttcaacg cgatcatggg cctggtgccc gtcaagagcg
     6961 ggcgcatcct attcgatggc cacgatctta cctgcctccc ggcccagtcc gctgccctcc
     7021 tcggtatcgg cttcgtgccg caaggccgta ggatctttcc cgaattcacg gtcatggaga
     7081 acctacgagt cgggaccagg accggacggg tcccactgga cacgctagac tggattttct
     7141 ccctgttccc gaggcttaag gaacgtctcg tgcaggtcgg gggaagcctt agcggcggtg
     7201 agcagcagat gctcgctgtg ggccgagcgc tcgccatgca gccccgtctc attctcatgg
     7261 atgagccaat ggagggcctg agcccgggca tggcggaggt ggtccgccat gccatcgtcg
     7321 aggcacgcga caagggcatg cgcatcctga ttattgaaca cgatattgac gaggctctcg
     7381 ccttggcgca ggacattcac atcatggaaa agggcagcat ccgggtccat tgccgtccgc
     7441 aacttctgat tcaggatccc gccattctcg aaaggcacct cggaatacgc ctgcacgccg
     7501 tatgatcctt ggtgagcgcc gcccgaaccg aaccaattct agggagcatg aacatgatca
     7561 agaagaatcc agataccacg gcaaacatgc gcaaggtcgt cggaacggat atgacccggc
     7621 gtcggctcgt cctcgccagc ggcgtcgcga tggccgctgc ggttgtcggc gcacccaccg
     7681 tactgagggc ggccaacaag ccgatcgtga tcggccatca gctcgatcag accggcatcc
     7741 tcgcggtata cgcgccctgg tacgatcggg ccgcgaaagg tgccatcgac cgcatcaaca
     7801 aggccggtgg catcgacggc cgtcaggtca aatacgtggt cgagaacacc gcctcgaagg
     7861 tcgacatcgg tttggcggcg atcgaaaagc tggtgaaaag ggaggagagc gacttcatcc
     7921 tcggcgccct gcattccggc attgcactcg gcagcacgaa agtctgcaaa agcttaaata
     7981 ctctctactt ctccgcggcg cacgctgagg aggtaaccgg gtctggcggc aatcggttcg
     8041 tgttccgtgt cagctcccag tcggaaagcg aaggcgaggc tacggcgacg aaggaactga
     8101 cctctgagct cgggtcgaaa tggtcgatct tctacgccga ttacgcctgg ggccaatctc
     8161 atgcccgcgt ctggcgcgag cggctgaagc gcaacggagc cgagatcctg agcgaggtcg
     8221 caatgccgct cggcgcgagt gacctggtac cgtacctttc caagatccac cgagacactc
     8281 aggcctgcgt cgtcgcgctg tttgcctcgg acgcggtcgg attcattcag cagcgggaag
     8341 ccctcggctt caatttcaag ctgatgggga tgcagggtct cgctgccggg atcccgccgg
     8401 acgttctgaa gctggccgcc ggcttctatg tccctgaaag cctcccgcgc cggctaaaag
     8461 acaaggacac cccccataac aagcttcttc gccagaccgt ccttgctgac gagaacggca
     8521 aagagatcgg cggcaaccaa tggctcaccg agtcctacta ttgggttgcg tggactcatg
     8581 tgaacctgat caaaaaggct gtcgaagcct caggctggaa gagcaaggcc gacaatctca
     8641 agatgatcga agccatggaa ggcatgaagc tcgaggaaag cgacgacttc ccgcagggct
     8701 ccacgaccat ccgggctgcc gatcaccaag cgttcgtaga cctgcatctg acccagatcc
     8761 aaagcgacgg gtttgccaag gtgatccgca agctcgcccc gaaacagtac ccggccccga
     8821 tcgactatcg caaggagacg ctctgacgcc acgcacagtt gcatgcggaa acgcgtaatc
     8881 gcagtcttcg cgcgcggaca ggttcgccga cgacaaaaga cagaagacag caaggagaat
     8941 aaaggcttgc tgcgatgtgc aagctgtaca gcctcagcaa gtgacaggcc gcacttaatc
     9001 gcgcgggtgc gcgcgatgcg tgacaccacc tgccctgact gggctctttt ggagcaagcg
     9061 ctgtgaggga atagcttctg gcgctgttcc gtaacctttc tgttcatcta aagttggaag
     9121 tgtcaccttc acgcctgtta ctaattctat atttagattt ttgaccgtgg agccgcccgc
     9181 aagtccctta aatgttaaga acattaaagg cgacctcaac aagtaataaa aatagtcctg
     9241 gcttatattt ttcttttttg cgctcaaaac aagccaaccg tcgtgaatgc acccggatgt
     9301 tttcaaaata tatggatggc caaaactcat cgaatttgaa agaagaaagt cgccggggtg
     9361 aaccatccgc gaccgtttca ccccactagg ctttatccgt ttcttggtct ttgtaatgta
     9421 tttcgagccc tctgatgcat cgctgatcat tatccaatta acgccatcag gactgtctgt
     9481 aacgtagtct aggatgggcc tcggtgaccc gccgcgagcg atgttgaaga tgtcaccaag
     9541 cgcaaccaat ggccagacgt cacacccaac gagtcgttcc gcggcaatat cccacctccc
     9601 taaaaaagcc cagctgtctg aaaaaatgcg cgatgtgttg tacacatgtt gtacgaagct
     9661 aatctcgtat gatacttttt gctatataaa ttctaggtac acaattgtaa ataaacacga
     9721 attataccaa cggccttaaa gaatgaccgt tgcccatgat cgggttgtga tggacgcgat
     9781 gcggttttgg tcgttggcga agaatgtcca ggcgtcgcat gccttgtcga ggatgtcgtc
     9841 gtaactgtcg aatacggtaa t
//
LOCUS       DASRKK010000783         7418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_867603, whole genome shotgun sequence.
ACCESSION   DASRKK010000783 DASRKK010000000
VERSION     DASRKK010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867603"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            634..1308
                     /locus_tag="VFC15_18820"
     CDS             634..1308
                     /locus_tag="VFC15_18820"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HZM12266.1"
                     /translation="MAERLATLFLLVVLSCSLTGCGVPRTKKKSSPPSDTAYADFDYF
                     LFTLSWAPEFCATNSNGRSSAECDAEKHMGLVVHGLWPQYDNGKWPQDCASTPPVASA
                     IVDHIMPIMPGEEFIQHEWVKHGSCSGLSTRDYFSEIEKLYTGLQFPNNFKKPPSSFK
                     TNPSDIEKEFATANYAPKGAFRVSCPQNRFSAVEICLSKDFQYQSCPNTVTECRANKI
                     VVRPVP"
     gene            1989..6713
                     /locus_tag="VFC15_18825"
     CDS             1989..6713
                     /locus_tag="VFC15_18825"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZM12267.1"
                     /translation="MTAAHRYPGIRVTANGNQLVSYHTETRIADAGVFYPITPSTEQG
                     ELFQQSYAEGRLNVFGGSSLAVEAEGEHAAQGGAIAHSVCGKRVVNFTSGQGILYGVE
                     QYYHAPGKCSTMVLEVAARALTKHALNVHCGHDDVYGALDTGWIILFGKDAQQAADQA
                     LILRRVTELSLTPGMNCQDGFLTSHLERTFYKHESELIRLFLGAPDDIIDCPTESQRI
                     LFGPKRRRVPRMIDLTNPVLLGPVQNQEHYMQGIAARRSNFTEPIAQFLEEAYADFGK
                     LTGRYYGMLTAYKADDADTVFVSLGSAAENIEAAVDYLRERRGAKVGSIHLNVIRPFP
                     EAAVVNALAGKKNVIVLERTDEAMAGDNPMGRDIRTALNKALQTGDRPATSGLPAITA
                     EQMPRLFSGIYGCGSRDFRPEQVFGAHEFATGARARTDGKLASDGTSFMVLGVDHPYE
                     VKSDETPSLLPKGAVAVRFHSIGGWGAITTGKNLGAIVGDLNDLLYERDKIVDELGNP
                     KEIIHVSANPKYGSEKKGAPTAYFLVAAPERIRVNCDLRHVTVVLCCDPKAFTHTNPL
                     AGMADGGCLVWESDETGEAAWERLPLWARKQVIDQNIRVFTLPGFQIARTATDRTDLQ
                     LRMQGNAFLGAFFAVSPLLGEFGITQEQFRDVVHKQYVKKFGQLGEAVVKSNMEVMTQ
                     GFEQVQEIRVGKLEAKDRSAMRGEALLPTVCGDNGDSCHFGCRAFPVPAGQAERTPLT
                     RIATFDAEFRAGYGYNQPSSALASVGVMAAATGDTASKYVARLVTPLFLPENCTQCME
                     CIAVCPDTALPNCSQDVETILHTAVTRYVTDPGERRKMLQSLPEIEKRVRQMMSDAVA
                     KNSGTPLPQILRQITAEVDGFSEEAKLQFFSIIDKVPMAYQKVNAIFSTPERKKPGAG
                     GIFSIFVTDLCKGCAACVTACGDHEALKMVQETEEVNAEHETGTGFLNLLPDTPQKYL
                     GLYSDERPQDSKTATLRNMLMVRHNYDAMVSGDGACAGCGEKQVLRAIAAATEAYMRP
                     LYHAKADRLRAKADELDKTGVGKLASLKQRNPEEYDLLRQAVAHLLMGLGGEDEKDTR
                     GRIACHGPISDQDIVGALTAVMRQEAFNHKELQAIDGRLADGMCVMAMAAHTGCNTVY
                     GSTPPNNPHPYPWMNSLFQDGVTVGWLLGESFIVDHGRRSVIPERLADALLGREKDVI
                     TPREYYEYAHFSDTLMTDQEIFELPKVWAVGGDGGIGDIGYQNTSKVILQNRPNVKVV
                     MLDTQLYSNTGGQNSDSTPMLGGSDMNVFGAATQGKGTEKKTVAETFLAGHGSPFVAQ
                     VSIANPPKLLRAILEGIEYRGTAFLQCFTTCQPEHGVGDDMALTQAQRVRDSRAAPEF
                     IFNPRLGETYQETLDLKGNPTLDTDWYEAKLKGGDEVSAYTVAHWCATEARFRNHLKK
                     IKPEQAAKLVPLENMLVRITQQDAVYRRYLQPEHRSYIPDFGVCIKIHGAQGQIDYRA
                     ISRQLVLYCVERRKAWRMLQSKAGIANKDYVAQKSILADVDAGKISREELFARGQYLV
                     KERQRPAGVVKPAEQKTGAVLA"
     gene            6782..6919
                     /locus_tag="VFC15_18830"
     CDS             6782..6919
                     /locus_tag="VFC15_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12268.1"
                     /translation="MGRSLETALGWNWPLLQFFPGAPTGIGPPSPARNWNWWLLQFSP
                     V"
     gene            7233..>7418
                     /locus_tag="VFC15_18835"
     CDS             7233..>7418
                     /locus_tag="VFC15_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HZM12269.1"
                     /translation="MQRTATAHWTGGLKDGKGTVSTQSGVLNQTQYSFSTRFENGAGT
                     NPEELIAAAHAGCFSMAL"
BASE COUNT         1578 a         2259 c         2152 g         1429 t
ORIGIN      
        1 tcgccaggtc taccaagatt cgctgcgtag caaagaagtt ttcaattccc tgctgcaccc
       61 atcccgatag cagattgata aaggacgact cccgcgcctg aggtgcgtgc tttgcatctt
      121 gtttcatgat agcctcctca attgctgcac tgcagcatta actaaactct agtattcggc
      181 cgattccatg tcaagttata aattaaacta ttgcaatgca gcacgcggca aatcgcggct
      241 gacgaagaga ggttgccttc atactcgccg caagccgcag gcattacggc acttgcgctc
      301 gctgggtgtt agatgggaag tgcatcgctg aataccccac aatgggaaaa ccatcgcctc
      361 caaggccgct gaccttgaac tggcaccgat tgcggcaaga aacgctgcgt atgctacgcg
      421 aagcccagga aaaagccaga gactatgccc gcgaacatcc ggtggaacgt gccattcctt
      481 agcgctttcc gcgtgtcctt tctggaggga atcagaggct acacgtggac atgaaagtgt
      541 gtcgctctgc tcttcgctct cgaccacttt gcccatcttc acaaatgcaa tttcactgac
      601 atgttgccaa aggtgcagta ccatgtgcac gctatggctg agagacttgc caccctcttc
      661 ctgctggttg tcctgagttg ttccctcacc ggatgcggcg tcccgagaac caagaagaag
      721 tcgtctcccc cgagcgacac tgcctatgcc gatttcgact acttcctgtt caccttgtcg
      781 tgggcgccgg aattttgcgc caccaattcc aacgggcgta gttcggccga atgcgatgcc
      841 gagaagcaca tggggctggt agtgcacggt ctgtggccgc agtacgataa tgggaagtgg
      901 cctcaggatt gcgccagcac cccgccagtt gcgtccgcta tcgtcgatca catcatgccg
      961 attatgccgg gggaggagtt tatccagcat gagtgggtca agcacggttc gtgcagcggt
     1021 ctatcgacgc gggactactt tagcgagatc gagaagctct ataccggatt gcagtttccg
     1081 aacaatttca agaaacctcc cagcagtttc aagaccaatc cgtccgacat tgaaaaggag
     1141 ttcgccacgg cgaactacgc tcccaagggc gcatttcgcg tctcatgccc gcagaacagg
     1201 ttctcagcgg tggagatttg tttgtccaag gacttccaat atcagtcgtg cccgaacacg
     1261 gtaacggagt gccgcgccaa taagatcgtg gtgcggcctg ttccgtaggg gagtaatgat
     1321 gagatgcgtc atgacacaga tgggctacca acggctaaat gaatttcggc catgggtttc
     1381 ggctgctccc gcgccacggt cgtacacgtt aagggaattt tccgctggtc tacagagggt
     1441 gaatcagcca cccccacaag tttctgaatg tgcacagtca gaaattgcgg tttagtgttg
     1501 gtcgataaac caaagatttg tcgccagctc gccgcccacg aggacgagat ctccacggca
     1561 cctatcacgg cgctggcaat ggtctcggcc catgtgctgc ccggcggtac gcccgaagct
     1621 cgcgctgcga ctggtgaatg ccacgagaaa agttcgctaa gtaaccgagg aacgcgtacc
     1681 cgttgctttc aacgttgaat ctagcatcga ctacgccgcg ggtttgttcg tcgctcgaga
     1741 ctaatctcga gaggcgacgc caacctattg caaatgaccc acttctacgt gaagccgact
     1801 acacacacaa gtgatttcgg gtgccctgtg taaatccgca tgctcacttc ctgatacggc
     1861 aacgatcact gacaaaccta agtctcaagg gtgaaaatcg tttcgcgtgg tgttcacgac
     1921 agaatatcgg cccttaaacc catctctgat gacacctccc cgagaggtgc acattcgcgg
     1981 aggaaatcat gactgccgca caccgctacc ctggcatacg agtcaccgca aacggcaatc
     2041 agctcgtctc gtaccatacc gaaacccgca ttgccgatgc tggcgttttc tatccaatca
     2101 caccttcgac cgaacaaggt gaactctttc aacagtccta cgccgaaggc cgcctgaatg
     2161 ttttcggcgg cagcagcctg gcagtggaag ccgagggcga gcacgccgcg caaggcggag
     2221 ccatcgcgca ctcggtgtgt ggcaaacggg tagtaaattt cacctccggc caaggcatcc
     2281 tgtacggcgt cgagcagtat tatcacgcgc cgggcaagtg ctcgacgatg gtgctggagg
     2341 tcgccgctcg ggcgctgacc aagcacgcgc tcaacgttca ctgcggtcac gacgacgtct
     2401 acggcgccct cgacaccgga tggattattc tgttcggcaa agacgctcag caggccgccg
     2461 accaggcgct catcctgcgg cgcgtgaccg aactgagcct gacgcccggc atgaactgcc
     2521 aggacggctt ccttacctcc catctggagc gaacttttta taagcacgag tcggaactga
     2581 tccgcctgtt cctcggtgct ccggacgaca tcatcgactg cccgaccgag agccagcgca
     2641 tcctgttcgg tccgaagcgt cgccgggtgc cgcgcatgat cgatctcacc aaccctgtgc
     2701 tgctcgggcc ggtgcagaat caagagcact acatgcaggg catcgccgcg cgccggagca
     2761 acttcaccga accgatcgcg cagttcctgg aagaagccta cgccgatttc ggaaagctga
     2821 ccggccgcta ttacggcatg ctcaccgcct ataaggccga cgacgcggac accgtgttcg
     2881 tgtcgctggg ctcggcggcg gagaacatcg aggccgcggt ggattatctt cgcgagcgca
     2941 gaggtgcgaa ggttggctcc atccacctca acgtgattcg ccccttcccc gaagccgctg
     3001 tcgtcaacgc gctagccgga aagaaaaatg tcatcgtact ggagcgcacc gacgaggcaa
     3061 tggctggcga caacccgatg ggccgcgaca tccggacggc gctcaataag gcattgcaga
     3121 ccggcgatcg tcccgcgacc agcgggctgc ccgcaatcac agccgagcag atgccccgcc
     3181 tcttctccgg catctacggc tgtggctcgc gggacttccg gcctgagcag gtcttcggcg
     3241 cgcacgagtt tgccactggc gctcgcgccc gcacagacgg caagctcgcc tccgatggga
     3301 cctcctttat ggtgctcgga gtcgatcatc cttacgaggt gaagtccgac gaaacgccgt
     3361 cgcttctgcc caagggcgcg gtcgcggttc gcttccactc catcggaggc tggggcgcaa
     3421 tcacgacggg caagaacctg ggcgccatcg tgggcgattt gaacgacctg ctttacgagc
     3481 gcgacaagat cgtcgacgag ttgggcaatc ccaaagagat cattcacgtc agcgcgaatc
     3541 ccaagtacgg ctccgagaag aaaggcgctc ccaccgccta cttcctggta gcggccccgg
     3601 aaagaatccg cgtcaattgc gatcttcgtc acgtgaccgt cgtgctttgt tgcgacccga
     3661 aggcattcac gcacaccaat ccgctggccg gcatggccga cggcggctgc ctggtgtggg
     3721 agtccgacga gacgggcgaa gcggcctggg agcggttgcc gttatgggcc cgcaagcagg
     3781 tcatcgacca aaacatccgc gtgttcacct tgccgggttt ccaaattgcg cgcactgcga
     3841 ccgaccgcac cgaccttcaa cttcgcatgc aggggaacgc cttcctgggc gcattcttcg
     3901 cggtctcgcc gctgcttggc gaatttggga taacccagga gcagttccgc gacgtggttc
     3961 acaagcagta cgtaaagaag tttggtcagc tcggtgaagc cgtcgtcaag tcgaacatgg
     4021 aagtgatgac gcaaggcttc gaacaagtcc aggagatccg cgtgggcaag ctcgaagcca
     4081 aagatcgctc cgcgatgcgc ggtgaggcac ttctgccgac cgtctgcggc gacaacggcg
     4141 atagttgcca cttcggatgc cgcgccttcc ctgtgcccgc tggccaggca gagcgcactc
     4201 cgctgacgcg aatcgcgacc ttcgatgccg agttccgcgc cggctacgga tacaaccagc
     4261 cgtcgtctgc gctggcttcg gtcggtgtca tggccgctgc aaccggcgat acggcgtcga
     4321 agtatgtcgc gcgtctcgtc accccgctct tcctccccga aaactgcacc cagtgcatgg
     4381 agtgtatcgc cgtctgcccg gatacggcgt tgcccaactg ttcgcaagac gtagagacga
     4441 ttctgcacac tgcggtcacg cgatacgtca ccgatccggg cgagcgcagg aagatgctgc
     4501 aatctcttcc cgagatcgag aaacgcgtgc gtcagatgat gtccgacgcg gtggccaaga
     4561 actcaggaac accgctgcct caaatcctcc gccagattac cgccgaggtg gatgggttct
     4621 cggaagaggc caagctgcag ttcttctcca ttatcgacaa ggtgccgatg gcctaccaga
     4681 aggtgaatgc catcttctcg accccggaga gaaagaagcc cggtgccggc ggcatcttct
     4741 ccatctttgt caccgatctc tgtaaaggtt gcgctgcctg cgtgaccgcc tgcggcgatc
     4801 acgaggcgct gaagatggtg caggagacgg aggaagtgaa cgcggaacac gagactggca
     4861 ctggcttctt gaatcttctg ccggatacgc cgcagaaata cctgggcctc tacagcgatg
     4921 agcggccaca ggattccaag accgcgacgc tgcgcaacat gctgatggtc cggcacaact
     4981 acgacgccat ggtcagcggc gatggcgcct gcgcaggctg cggcgagaag caagtgctgc
     5041 gcgcaatcgc tgccgccacc gaggcctata tgcggccgct gtaccacgcc aaagccgacc
     5101 ggctgcgagc caaagcagac gagctcgaca agacgggagt tgggaagctg gcttcgctga
     5161 aacagagaaa cccggaagag tacgatctgc tgcggcaagc ggtcgctcat ctcctcatgg
     5221 gactcggtgg cgaggatgag aaggacaccc ggggacgcat tgcgtgccat ggaccgatct
     5281 ccgaccagga cattgtgggc gcgctcacgg ccgtgatgcg gcaggaggcc ttcaaccaca
     5341 aagagctcca ggcgattgac ggccggcttg ccgacggcat gtgcgtcatg gcgatggccg
     5401 cacataccgg ctgtaacacg gtctacggct cgactccacc gaacaaccca catccttatc
     5461 cctggatgaa ctcgctcttc caggacggcg tcaccgtcgg ctggctgctg ggcgagagct
     5521 tcatcgtcga tcatggccgt cgctccgtta tacccgagcg gcttgccgat gcgctgctcg
     5581 gtcgcgaaaa ggatgtcatc acgccgcgcg agtactacga gtacgcgcac ttcagcgaca
     5641 cgctgatgac cgatcaggag atttttgagc tgcccaaggt gtgggcggtc gggggcgacg
     5701 gtggcatagg tgacatcggg tatcagaaca cctcgaaggt gattctccag aatcgcccca
     5761 acgtgaaagt ggtcatgctc gacacccagc tctactccaa caccggagga cagaactccg
     5821 actcgacgcc catgctcggg ggcagtgaca tgaacgtgtt tggcgccgcg acccagggca
     5881 agggcaccga gaagaagact gtggccgaga ccttcctggc cggacacgga tcgccgtttg
     5941 tcgcccaggt ctccatcgcc aacccaccga agctgctgcg tgcgattctc gaaggtatcg
     6001 agtatcgcgg gacggcgttt ctgcagtgct tcacgacctg ccagccggag cacggcgtgg
     6061 gcgacgacat ggccctcacc caggcccagc gcgtgcgcga ctcgcgtgcg gcaccggaat
     6121 ttatcttcaa tccgcggctg ggggaaacgt atcaggaaac gctcgatctc aagggcaatc
     6181 ctacgctcga caccgactgg tacgaggcca agctgaaggg cggcgacgag gtatctgctt
     6241 atacggttgc ccactggtgc gccaccgaag cccggtttcg caaccacctg aagaagatca
     6301 agccggagca agcggccaag ctggtcccgc tggagaacat gttggtccgc atcacccagc
     6361 aggacgccgt ctaccgtcgc tacctgcagc cggaacatcg ctcgtatatt cccgacttcg
     6421 gcgtctgcat caagatccat ggcgcacaag ggcagatcga ctaccgggcg atttcgcggc
     6481 aactagtgtt gtactgcgtc gagcgccgca aagcgtggcg catgctgcag agcaaggctg
     6541 gcatcgcgaa caaggactac gtggcccaga aatcgatcct ggccgacgtc gatgccggca
     6601 agatttcccg ggaggaattg ttcgcgcgcg ggcagtacct ggtgaaggag cgccagcggc
     6661 ccgccggcgt ggtgaagccg gccgaacaaa agacaggtgc ggttttggcc tagtcgcaaa
     6721 ccgcgctgca ccccggttcc ccataacttg cacgattcgg gtggaacccg ggcggaatcg
     6781 agtggggcgc tctctggaaa ccgctctcgg ctggaactgg ccgttgttgc agttcttccc
     6841 gggtgcgccc accggcatcg gaccgccgtc gcccgctcgc aactggaact ggtggttgtt
     6901 gcagttttcc ccggtgtgac gcgagtcaac ccgagccaca cgcccccgcg ctgcttctgc
     6961 gactaaaccc gccccgaacg catgcttgcc ggctttgcag gtttgtccca actgttcagc
     7021 tacgcgcggg aacaacattg taagctggtc tgtccgcagt gcgggtattt cctcagctgt
     7081 tcggacttcc attgaagcac ttgcaagcag atcaccagaa tcccactaac aagaccgagt
     7141 gactatgggt tctcgctctc cgtttcacgc aagatttacc atgttcgggt atcgtagaga
     7201 tagaattaac tcgattccca ggaggataaa gaatgcaacg cacagcaact gctcactgga
     7261 cgggtggttt gaaagatggc aagggaaccg tttccacaca aagcggggtc ctgaaccaaa
     7321 cgcagtactc cttcagcacg cgttttgaaa atggcgccgg tacaaatcct gaagaactga
     7381 ttgcggcggc gcacgccggt tgtttttcca tggctctt
//
LOCUS       DASRKK010000784         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_771387, whole genome shotgun sequence.
ACCESSION   DASRKK010000784 DASRKK010000000
VERSION     DASRKK010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771387"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFC15_18840"
     CDS             <1..310
                     /locus_tag="VFC15_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZM12270.1"
                     /translation="GPLSAPLHLIMDGAYEDNQTLQLALDFGFIPVVPPRPNRLEPWQ
                     YDKAMYRRRNEIERLFRRLKGFRRIFSRFDKLDLVFLGFLHFALIVEALRYLNSVNTP
                     "
     gene            561..>2798
                     /gene="mgtA"
                     /locus_tag="VFC15_18845"
     CDS             561..>2798
                     /gene="mgtA"
                     /locus_tag="VFC15_18845"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZM12271.1"
                     /translation="MGKPTASVESFWDRPLHELFELLPATPAGLTTEEARRRLRLYGP
                     NSFVHESRFAALLSFFGFFRNPLVVILVVASGVSLALGEHIGGLIILAIVLFSVILNF
                     LMEFQARHAVEDIQKQIALTAAVVRDGREQELPTAELVPGDIVRLNAGDLVPADARLL
                     EVKDLHMRESVLTGESLPVEKTVADLPKEKHSVADASNSVFLGTAVQTGIGTAVIVCT
                     GKDTACGEIANRLAMRPPETEFGRGIRHFGMMLTWVTMLLVLFVLLVNIIFHRPLLES
                     FLFSVALAVGMTPEMMPMIITVTLAQGAKRMTKKKVLVKQLSAIEDFGSVDILCTDKT
                     GTLTEGEIVLDRHVDFQGKDNENVLQLVYLNSHFEAGIKSPLDDAILKHEAPSIVGYD
                     KVDEIPFDFNRKRLSVVVRHEDEDLLITKGEAESVFAICETVTIDGVPQPFDEKRRAE
                     AEDTLKKLSADGFRVLGVATLKVQKQNAYAVAAEHSMTLVGFAAFLDPPKEGVLAVLQ
                     ALKQNGISVVVMTGDNQYVTQKIAHDVGLNADRILVGNQLDTMDDAAVAHQAENGAIF
                     ARVSPEQKNRVILALKARGHVVGYMGDGINDAPSLHTADVGISVMNGVNVAKDAAKII
                     LLEKDLAVINEGVLEGRRSFANIMKYIIMGTSSNFGNMFSMAAASLFLPFLPMLPSQI
                     LLNNFLYDVSQVSIPTDNVDPAMVQKPKRWQIGFIRQFMAIIGPISSIYDFLTFGVLL
                     WVFHAA"
BASE COUNT          590 a          811 c          795 g          602 t
ORIGIN      
        1 cggaccgctg tcggcgccgc tgcacctgat catggacggt gcttacgagg acaaccaaac
       61 gctgcaactg gctctggact tcggtttcat cccggtggtc ccgccccgac cgaaccgtct
      121 ggaaccctgg caatacgata aggctatgta ccgcaggcgc aacgaaatcg agcggctgtt
      181 ccgcaggctc aagggctttc gtcgcatctt ctcgcgcttc gacaaactcg atctggtctt
      241 tctcggcttc ctccactttg ctctaattgt cgaagcgctc aggtacttaa atagtgttaa
      301 cacgccctag agcgattccg ggattagtgt gtccacccgc acgggtagtg cggcgcttca
      361 gcgccgcgtt tgccgcgccc aaacgcctcg cttaagccgc tgaggtcgaa gacctcagcg
      421 gcttaagcga tttttcttct tgcgctttac gcgggcctga aggcccgcac tacccgtgca
      481 aaccagacac atctattgcg gaacggctct agtgagcaac gctctcatgg gataatgcgg
      541 cagttactgg agggacccgg atgggaaaac caacagcctc tgtagagagc ttctgggatc
      601 ggccgctgca cgagctgttc gaactgctgc cggcaactcc ggcaggactg acgacggaag
      661 aggcgcggcg gcgactgcgt ctttacggcc caaattcctt cgtccacgag tcccgtttcg
      721 ctgcactgct cagtttcttt ggtttcttcc gtaatcctct cgttgtcatc cttgtcgttg
      781 ccagcggcgt gtcgcttgcg ctcggagagc acatcggcgg cctgatcatc cttgccatcg
      841 tactgttcag cgtaatcctg aattttctga tggagttcca ggcccggcac gccgtggagg
      901 acattcagaa acagattgcc ctgactgcag cggttgtgcg cgatggccga gagcaggaac
      961 tgccgaccgc cgaactggtt ccgggagata ttgtccggct caatgcagga gacctggtgc
     1021 cagccgacgc ccgtttgctg gaagtaaagg acctgcacat gcgcgagtct gtgctgaccg
     1081 gtgagtcatt gccggtcgag aagaccgtgg ctgaccttcc caaggagaag cacagcgttg
     1141 cggacgccag caacagcgtt tttctaggca ccgcagttca gacagggatt ggcaccgcgg
     1201 tcatcgtctg cactgggaaa gacaccgcct gcggtgagat tgccaatcgt ctagctatgc
     1261 ggccgccgga gaccgagttc ggccgcggca tacgccactt cggaatgatg ctgacctggg
     1321 taaccatgtt gcttgtcctt ttcgtcctgc tggtgaacat cattttccat cgcccgctgc
     1381 tggagtcttt ccttttctcg gtcgcacttg ccgtcgggat gacgcctgaa atgatgccca
     1441 tgatcatcac ggtgacgctg gcccaaggcg ccaagcggat gacgaagaag aaggtgctgg
     1501 tcaagcaact gtcagcaatc gaggatttcg gcagcgtgga tattctctgt accgacaaga
     1561 cgggcacact caccgaaggg gaaatcgtgc ttgaccggca cgtggatttc cagggtaaag
     1621 acaacgagaa cgtcctgcag ctcgtttacc tcaacagtca ctttgaggcg ggcatcaaaa
     1681 gcccccttga cgatgccatc ttgaagcacg aggcgccttc catcgtggga tatgacaagg
     1741 tggacgagat tccgttcgac ttcaaccgca agcgcctttc cgtggtggtt cgacacgagg
     1801 acgaggacct tctcatcacc aagggtgagg ctgagagtgt gtttgccatt tgcgagacgg
     1861 tgaccatcga tggcgttccg caaccgtttg acgagaagcg gcgggcagag gcagaagaca
     1921 ccctgaagaa gctgagtgcg gacggattcc gcgtgcttgg ggtggcaacg ctcaaggttc
     1981 aaaaacagaa tgcatacgcc gtcgcagccg aacactcgat gacacttgtc ggtttcgcgg
     2041 cattcctcga cccgccgaaa gagggagtac ttgccgtcct gcaagccctg aaacagaacg
     2101 gcatttccgt ggttgtcatg acgggcgaca accagtacgt gacccagaaa atagcccacg
     2161 acgtagggtt gaacgcagac cgcattcttg tcggcaacca gctcgacact atggacgatg
     2221 ccgccgtggc ccaccaggcg gaaaacggcg ctatattcgc ccgcgtatcc cccgagcaaa
     2281 agaaccgggt aattctcgct ctcaaggccc gcgggcacgt cgtgggatat atgggcgatg
     2341 ggatcaacga tgctccgtcg ctgcacactg cggacgttgg catctccgtc atgaatggcg
     2401 taaacgtcgc caaggacgct gccaaaatca tcctattgga aaaggatctt gcggtcataa
     2461 acgagggcgt gctcgagggg cggcgctcct ttgccaacat catgaagtac atcatcatgg
     2521 gtacgagctc gaatttcgga aacatgttca gcatggccgc ggcatcactc ttcctgccct
     2581 tcctgccgat gctcccgagc cagatactgc tgaacaactt tctctatgat gtttcgcagg
     2641 tcagcatccc caccgataac gtcgatcccg ccatggtgca aaagcccaag cgatggcaga
     2701 ttggattcat ccgccagttc atggcgatca tcggtccaat cagctcaatt tatgatttcc
     2761 tcacgttcgg ggtgctgctc tgggtgttcc atgctgcc
//
LOCUS       DASRKK010000785         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_184162, whole genome shotgun sequence.
ACCESSION   DASRKK010000785 DASRKK010000000
VERSION     DASRKK010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184162"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1602
                     /locus_tag="VFC15_18850"
     CDS             64..1602
                     /locus_tag="VFC15_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12272.1"
                     /translation="MASEPVASSIPPIHKRVVNIQRQEHDSSCQFADGTKFLLPRILS
                     PYLTIGDEITFPIPSTDAVGPEILVSKNAASGSSRYIYQTPIGYVTQPKQDKRNQHFV
                     SAEVKRGSLGIGTIFLPCEVLREYFYRLPHTADRVDHPTLYEVLRIPPEASPSELRVA
                     FKLRDLELQTAGVRHRERVALERAFNIVGQPELRACYDALLADPEAPAIFPYGGFGSL
                     LVAGEPSRDGATFFARRIIAFSPDLRRRRFHVPLRRCDFYDDRALCRDVRRKLELWLD
                     PAALHTSWDRTWNQWKHLLETKIEVEGTFVQSGKYRKRRDQWELVTWETALPSRLEVK
                     LPPDFHLQIESAKTAYHRFGQYSRALEQIRLCLEHRAVERAELERMCAELRLPGDFDV
                     AQISWRPDYDPFFYRQLSRRARQIYLFREEYIFDVEKAVVVETPQLGHATYVFAKPRS
                     MDTFLALYTKITKDDIRQNRNNAAEQLGFLGRVIHGTNPRAWLKEVRQRVGEGIDYAA
                     AVAE"
     gene            2072..2317
                     /locus_tag="VFC15_18855"
     CDS             2072..2317
                     /locus_tag="VFC15_18855"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM12273.1"
                     /translation="MFIQPSLASSQLHFDREPFVAADRAAAYLGVTRRTLLHKARLGK
                     IPAYPLDPGAKKKEWRFKLSELDRHVCASVNSCLQPT"
BASE COUNT          478 a          702 c          632 g          514 t
ORIGIN      
        1 tctcgcatcc catgagtcgc gttttgcgct ttcgcaatcc gctcctatac tgcccacgaa
       61 ctcatggctt cagagcccgt cgcttcatcg attccgccga ttcacaaacg cgtcgtgaac
      121 atccagcggc aagaacatga cagttcttgt cagtttgccg acggaacgaa gttcctcttg
      181 ccgcgcattc tctcccccta cttgaccatc ggagacgaga tcacatttcc cattccatca
      241 acggatgcag tgggcccgga aatcctcgta tccaagaatg ccgcctccgg atcgagccgt
      301 tatatctacc agacgccaat cggatatgtc acccagccca agcaggacaa acgaaatcag
      361 cacttcgttt ctgccgaggt taagcgagga agccttggca tcggcacgat ttttctgcct
      421 tgcgaggttc tgcgcgagta cttctaccgc ctgccgcata cggccgatag ggtcgatcat
      481 cccactctgt acgaagtctt gcgcatccca cccgaggctt cgccttccga actccgcgtt
      541 gctttcaagc tgcgtgatct ggagctgcaa acagcgggcg tgcgacacag agagcgagtg
      601 gccctggagc gggccttcaa catcgttggt cagcccgaac ttcgcgcctg ctatgacgcg
      661 ctgctggccg atcccgaggc accggccata tttccttatg gcggattcgg gtctcttctt
      721 gttgccggag agccttcccg tgacggcgcg acgttctttg cccgccgcat catcgcgttt
      781 tcaccagatc tccggcgccg acggttccac gtcccactgc gaagatgcga tttctacgac
      841 gaccgggcac tatgccgcga cgtacgtcgc aaactagagt tgtggcttga cccagccgcc
      901 ctgcatacaa gctgggaccg cacctggaac cagtggaaac atctgttgga aaccaagatc
      961 gaagtcgagg gcacgttcgt acagagcggc aagtaccgca aacgccgtga tcagtgggaa
     1021 ctcgttacct gggaaactgc gttgccgagc cggttggagg tcaaactgcc acccgatttc
     1081 catctgcaaa tcgaatctgc caaaactgcc tatcatcgct tcggccagta ttcgcgcgct
     1141 ctggagcaga tccggctgtg cttggaacat cgggcggtcg agcgagcgga gctggagcgc
     1201 atgtgtgccg agctgcgtct tcccggtgac tttgatgtcg cccagatcag ctggcgtcca
     1261 gactatgatc cgttcttcta tcgccagctc tcacgccggg cacgccagat ctatctgttt
     1321 cgggaggaat acatttttga cgttgagaaa gcggtggtcg tcgaaacccc tcagcttggc
     1381 cacgcgactt atgtctttgc caagccccgg agtatggata cctttctggc tctgtacacc
     1441 aagatcacga aggacgacat ccggcagaac cgcaacaatg cagccgagca gttgggcttt
     1501 ctggggcgcg tgattcacgg caccaaccca agagcctggc tcaaggaagt caggcagcgg
     1561 gtaggggagg gaattgatta tgcagcggcc gtggcggagt aaattacggt cccatgcgtc
     1621 cccacttttg tggcatttag tttggcggcg cattcctgcg caccagttgc tttgcccagt
     1681 caaagtgtaa tcggtgaaat aaagttaggc gcccgtatgc ggactggaat tccgctcgtc
     1741 ggacgatgat gacggcggag ctgtttcctt cttcggatgt ggcccacgat gtcgagcgga
     1801 acccggcact cgcagcaaga acctggcaat cgtcttgcca ttcaactagc ggttgcgcgc
     1861 gccatagtgg cgacaaatcg tgcggatgtg gcgcgaaaac cacaccccgc agtcattctc
     1921 ggcggaaacc gagtcactga atcctgcagg gagcgagtcg ctgcgttgct cagtcgaaac
     1981 ctgccctgga ctctgatttg acgaattaac tttttgggct agaaatgcac ttcacggggc
     2041 cttcgttctg accccaactt tggagttgat tatgttcatc caaccctctc tagcgtcgtc
     2101 ccaactgcac ttcgaccgtg aaccatttgt tgccgccgac agggctgccg cgtatctcgg
     2161 tgtgactcgc cgcacgttgt tgcacaaagc ccggcttggc aagatcccgg cttacccgct
     2221 ggatcccggc gccaagaaga aagagtggcg attcaaactc tcggagctag accggcacgt
     2281 atgcgcttcg gtaaactctt gcctgcagcc cacctgagcc atcgca
//
LOCUS       DASRKK010000786         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_380693, whole genome shotgun sequence.
ACCESSION   DASRKK010000786 DASRKK010000000
VERSION     DASRKK010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380693"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..706)
                     /locus_tag="VFC15_18860"
     CDS             complement(95..706)
                     /locus_tag="VFC15_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12274.1"
                     /translation="MLVQAQQGPLVTKPAADLSIPAELTKTVRADKTHRGDPVEFTTL
                     EAVLVGPKLAMPPQSKLFGRVVGAAPRQGEKPSWLVLLVERAEQITLVAASNQNINVA
                     DATAATSNPRRPGRMSGRIAAQSDTSLLSLTKPPQDATEQSRDQLATRPATLKDVRIV
                     RDKDGTSYLFSAKSDVKLPSGILFMLQNRPTTAPDQSAAAGPH"
     gene            1049..1267
                     /locus_tag="VFC15_18865"
     CDS             1049..1267
                     /locus_tag="VFC15_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12275.1"
                     /translation="MARLNVYIFHCSRCGEPAYSLLKERTPGGVESGDTIFPTIDLKA
                     IRAQCSACKHTQFRDAEEKAHFALVEWN"
     gene            1317..1553
                     /locus_tag="VFC15_18870"
     CDS             1317..1553
                     /locus_tag="VFC15_18870"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZM12276.1"
                     /translation="MKYHTVHEAKTNLSELIRKACDGEQVIIARGRKPVVKLVPVGDA
                     ARERIPGGFEGKISWIADAFDPLTDKEMRDLGFE"
     gene            1550..1933
                     /locus_tag="VFC15_18875"
     CDS             1550..1933
                     /locus_tag="VFC15_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM12277.1"
                     /translation="MNLLIDSHALLWWFGATDKLSSRARDALKSPANRVYVSAAVAWE
                     LAIKANLGKLDASELLSDLPRVLFEKGFRRLAISMDHALRAGTLPRHHSDPFDRMLIA
                     QAQALGFAIVSADAEFDHYGVARIW"
     gene            complement(1981..2565)
                     /gene="ruvA"
                     /locus_tag="VFC15_18880"
     CDS             complement(1981..2565)
                     /gene="ruvA"
                     /locus_tag="VFC15_18880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZM12278.1"
                     /translation="MIAHLRGRLIAKHPNQAIVEAGGVGYDVTITVPTFSELPALGSE
                     VALHIHTHVREDALALFGFLRREEKQLFEKLITVSGIGPKLAITILSGMAAADMVGAI
                     RGNDFARLTRIPGIGKKTAERMCVELRDKLEGFGAAQPAVTHSAVEEDVISALSNLGY
                     QRAIAEKAVERAVQSTGRENFDAMFRAALGALSK"
     gene            2611..>3123
                     /locus_tag="VFC15_18885"
     CDS             2611..>3123
                     /locus_tag="VFC15_18885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZM12279.1"
                     /translation="MPKRYDATGKSTLPAGLFTATMLRRARILPREFFNRDPVTVGRE
                     LLGKLLIRREGRALLAGRVVEDEAYLGVGDPAAHAYCGRTPRNSVLFGPPGHAYVYFI
                     YGNHYCLNVSCMPEGYAGCVLLRAMEPVFGLPAMARARRLELAATPRPTQLRLISSGP
                     GRMSEALGITR"
BASE COUNT          620 a          937 c          944 g          622 t
ORIGIN      
        1 gaatagcggg atggaaatcc tgcataaaca gcgtaggcaa cggcaaagat aaggttctgc
       61 attggaaagt aatgtcaggc tcagtatccg gcgtctagtg tggccctgcg gccgcagatt
      121 gatccggcgc ggtagtgggc cggttttgca gcatgaagag gataccgctg gggagtttca
      181 catctgattt ggccgagaac aggtacgagg ttccatcttt gtcgcgaacg atgcgaacat
      241 ctttgagcgt ggccggtctt gttgcgagtt ggtctcgcga ctgctcggtc gcatcctgcg
      301 gaggcttggt caacgataac aggctcgtgt cagattgagc ggctatccga ccgctcatgc
      361 ggcccggccg gcgcggattg ctggtagcag ctgtcgcgtc cgccacgttg atgttctggt
      421 tcgaggccgc taccaaggtt atctgctcgg ctctctccac cagcaacacg agccacgatg
      481 gcttctcccc ttgacgtggg gcggcgccca cgactcggcc gaacagcttg ctttgaggcg
      541 gcatcgccag ctttgggccg accagtacgg cttccagagt tgtgaattcg acgggatcgc
      601 cgcgatgagt tttgtccgcg cgcaccgtct tcgtgagttc cgccgggatg gacagatctg
      661 cagccggttt ggtgacgagg gggccttgct gcgcttgaac gagcaatgca gccatcatca
      721 acagcgatat gcaccccaca cgcaatacga cggaccgcga ccgagtgcta ccacctggcc
      781 gaggaccgag gagtagggca gaaacattca aaaccccaac cgccatcaac cctgattaga
      841 gagtcttcgt gagaaccaac tcttcgcgta gcgcagtgga acgagccgca gcaatccagc
      901 cgcacagcgg cgcaatggga ttaggccggg gcgtgagccc cggcgacgcg catccgacga
      961 accgagcgca gagcggcgac accggttgcg acgcaccacc acgcacaggc gataggcata
     1021 taatccggcc atccaccgga gggtcgccat ggcgcgtctg aacgtttaca tcttccactg
     1081 ttcgcgatgc ggcgagcccg cctattcgct gctgaaggaa aggacgccgg gcggcgtcga
     1141 atccggtgac accatctttc ccaccatcga tctcaaggcc atccgcgccc aatgctccgc
     1201 ctgcaagcac acccagtttc gcgatgcgga ggaaaaggcc cactttgcac tggtggagtg
     1261 gaactagaag ctctgctact tgacaaacca agattagcta actaaattag ctaatcatga
     1321 aatatcatac tgttcacgaa gccaaaacca atctgtcgga gctcatccgc aaggcctgcg
     1381 acggggagca ggttatcatc gcgcggggcc gcaaaccggt agtgaagttg gtgccagttg
     1441 gcgatgcggc ccgggagaga atccctggag gattcgaggg gaagatctcg tggattgccg
     1501 acgcattcga ccccctcacc gataaagaga tgcgggacct ggggttcgaa tgaaccttct
     1561 tatcgatagt catgcgctat tgtggtggtt tggagccacg gacaagctct ccagcagggc
     1621 gcgtgatgcg ctgaagagtc ccgccaatcg ggtgtatgtc tcggctgccg ttgcctggga
     1681 gcttgccatc aaggcaaacc tcggcaaact cgacgcaagc gaactgcttt ctgatcttcc
     1741 ccgcgtgttg ttcgagaaag gcttcagacg gctggcaatc tcgatggatc atgcgctgcg
     1801 cgcaggaacg cttccccggc accacagcga tcccttcgac cgcatgctga ttgcgcaggc
     1861 gcaagctctc ggttttgcca tcgtgagtgc ggacgcggag ttcgaccact acggagttgc
     1921 aagaatctgg tagtcgttgg gtctaagcaa agcgcccgtt tgatttctcc acagcggaat
     1981 tcatttcgac agcgccccca gggccgctct gaacatggcg tcgaagttct cccgcccggt
     2041 cgattgcacc gcgcgttcca cggccttctc tgcaatcgcg cgctggtagc ccaggttcga
     2101 cagcgccgag atcacgtctt cctccaccgc ggaatgtgtc accgcgggtt gcgccgcgcc
     2161 aaacccttcc agcttgtcgc gcagttccac gcacatgcgt tcggcagtct tcttgccgat
     2221 tcccggaatt cgagtaagcc gcgcgaaatc gttgccgcgg atcgcgccga ccatgtccgc
     2281 cgccgccatg ccgctgagga tggtaatcgc cagcttgggc ccgatgccgc tcaccgtgat
     2341 gagcttctcg aagagctgct tctcttcccg ccgcagaaag ccgaagagcg caagagcatc
     2401 ttcgcgcacg tgcgtgtgaa tgtgtagcgc gacctcgctg cccagcgcgg gcagttcgga
     2461 aaacgtgggc acggtaatag taacgtcgta accgacgcca ccagcctcga cgatggcctg
     2521 gttcgggtgc ttggcgatga gccgtccgcg aagatgcgcg atcatgcagg atgattgtaa
     2581 attgatcgcg aacatcggat gctaaatgcg atgccaaaac gctacgatgc caccgggaag
     2641 tccacgctcc cagcaggtct ctttacggcc acgatgctga ggcgtgctcg gattcttccc
     2701 cgtgaattct tcaatcgcga tccggtgacc gtgggacgcg agttgctggg taagctgctc
     2761 attcggcgcg aaggacgagc gctcctcgct ggacgtgtcg tcgaagatga agcctacctc
     2821 ggagttgggg accccgcggc gcacgcttat tgcgggcgca cgccgcgcaa ttccgtcctc
     2881 ttcgggccac cggggcatgc gtacgtgtac ttcatttacg gcaatcacta ttgcctgaat
     2941 gtttcctgca tgcctgaagg atatgccggc tgcgtgctgc tacgggccat ggagccggtg
     3001 tttggactgc cggcgatggc ccgggcccgc cgcctcgagc tggcggccac gccgcgaccc
     3061 acgcaattgc gactgatctc tagcggcccc ggacgcatgt cggaggccct cggcattacg
     3121 cgt
//
LOCUS       DASRKK010000787         5591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_184422, whole genome shotgun sequence.
ACCESSION   DASRKK010000787 DASRKK010000000
VERSION     DASRKK010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184422"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..696
                     /locus_tag="VFC15_18890"
     CDS             238..696
                     /locus_tag="VFC15_18890"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM12280.1"
                     /translation="MIKSFGGGETERLWKEERASNWYVALGTTAGMDWYDLTSVSLPY
                     GTSGTYFTSSTTSGDAISIWGFNGGQPQVGLTTGYTSGTPISADMTIAGETIAGMTLI
                     PGSYTFTLPSDTIILNIAGTTSVPEPSTWTMMLSSFGAFGLAGYRRLRKA"
     gene            complement(1154..1519)
                     /locus_tag="VFC15_18895"
     CDS             complement(1154..1519)
                     /locus_tag="VFC15_18895"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZM12281.1"
                     /translation="MSTTRILVLMDFEIYDGKSDEFQAIAKQMVEEFEQEPGTLAYHF
                     VLSTDRKRCRLVQGYADQAAINAHWIDGPAVHQNIFQFNQVAVTTRVEVYGDPGPQVA
                     ALAAAFGAEIFTWWEGFDR"
     gene            complement(1624..2088)
                     /locus_tag="VFC15_18900"
     CDS             complement(1624..2088)
                     /locus_tag="VFC15_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12282.1"
                     /translation="MKIRSIVVTFVLCLLALTVGFAQNPNLGTWKLNEAKSRIPAGVG
                     KNTTVVYSAEGSDIKVTTDGVNATGQPAHSEWTGKFDAKPYPVTGDPNVDYRAYKAKG
                     ERTLLPANMKGDKTVSNGKIELAKDGKSRTLEMTYFAAGGKKIHAKYVYDKQ"
     gene            complement(2206..2595)
                     /locus_tag="VFC15_18905"
     CDS             complement(2206..2595)
                     /locus_tag="VFC15_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZM12283.1"
                     /translation="MDPDYYLLKTEPSEYSFADLQRDKVTTWDGVTNPVAVRNLREME
                     PGALLVIYETGDHKSAVGTASVVSVDAADPKKPLVKIKVGKPLPKPVSLAEIKANKLF
                     ADSPLVRIGRLSVVPLTKPQYKALTGE"
     gene            complement(2674..3735)
                     /locus_tag="VFC15_18910"
     CDS             complement(2674..3735)
                     /locus_tag="VFC15_18910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM12284.1"
                     /translation="MQLNEYVTLGRSGLRVSPLSLGPMTFGNDRWGSDDAESRRIFDR
                     YVENGGNVMDCADVYSGGKSEEILGRFVAEVGLRDKLVIASKFSFNPQDGNPNSGGNG
                     RKNIYRALESSLRRLKMDYLDLYWMHVWDTVTPVEEVVDTLSNLVREGKIRYYGLSDV
                     PAWYAARGQTLAACLGREPIVALQLEYSLVERTIEREHIPAARELGMGVCPWSPLASG
                     FLAGKYTREKGAESSQGSRLEVLKKADNPAFKKFNERNFSILDALLEVAKALNRPAAQ
                     VALNWVATQPGVTSTIIGATKVAQLDDNLAALSFEIPAELRAKLEAASQLEAAHPYMF
                     FSGVLQDRVSGGVKVRRWS"
     gene            complement(3852..5093)
                     /locus_tag="VFC15_18915"
     CDS             complement(3852..5093)
                     /locus_tag="VFC15_18915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM12285.1"
                     /translation="MAANGKNGRRYPKLVLGETVSVAIETFRSNRARFALTALGMVIG
                     TASLILVVTIGLTGKQYVLQQIQSIGANMITADYEGGSIYSAESANDYMTIEDLNAVR
                     QQVPNITASSPMVELHDRIAVPGGRERDVLVLGIAAEYLWVRNLEVLTGRFLEDGDSQ
                     TRGKVAAITQNLANSLYGSTDAAVGQVIKLSGLPFVVVGTFRERVDTFGQSEISDDTI
                     LIPYTVARFFTTTDTVKSLFFSVADSSDIPGATEQIQKVLHSRHRPESVYKVENLTLL
                     IAVAGKIANALTTILLLIAVLTLLVSGVGIMNIMLATVNSRIREIGVRKAVGATKAEI
                     RTQFLTEAILISLIGGFAGIVIGLALPISVRVLTNYRIPISGLSVVIAVLVSSLIGVL
                     FGTMPAARAAQLDPVESLRYE"
     gene            5238..>5591
                     /locus_tag="VFC15_18920"
     CDS             5238..>5591
                     /locus_tag="VFC15_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12286.1"
                     /translation="MSQFLHRSALTQTFIGALLFAIAAGTALAQQNVGELFASDASVK
                     GSVVLANSGTTVMSGSSIAAGAQSATLKLERGGSMIICPGTNLAITTSQNGRQLMFSM
                     DTGNIEMDYPIGAAAD"
BASE COUNT         1091 a         1652 c         1658 g         1190 t
ORIGIN      
        1 ggttagagag tggggagggg ggagtctatg aaataggggg cggcgatgtt acgaaggtaa
       61 cccggcaaat gccaaacgcg ggccacgagc gaagaaatgc cccaccctac cttcggtaga
      121 atggggcacc caagttaaat ttttggttgc ccagaccacc cgccagttgt cggttgtcag
      181 tgccaccagc ctatccccga ctgacgttac taacgcgtag cattagtatt gtatggcgtg
      241 atcaagagtt ttggaggcgg agagacggag cgcttatgga aggaagagcg tgcttccaac
      301 tggtacgtag cgctgggcac aacggcgggc atggattggt acgacctgac gagcgttagc
      361 ctgccctatg gtacgagcgg cacatacttc acttcgtcca caacctcggg ggacgccatc
      421 tccatctggg gattcaatgg gggccagccg caggtcggat tgacaactgg ttacacgtcc
      481 ggcactccga tttccgctga catgacgatt gcgggcgaga ccattgccgg catgaccctg
      541 attcctggat cctatacgtt cactcttccc agcgacacga tcatattgaa tattgctggc
      601 actaccagtg tccccgaacc ctcgacttgg acgatgatgc tgagcagctt cggagctttc
      661 ggcttggccg gctatcgccg gttgaggaag gcctgagcgc tcgcctggtt gcccacattt
      721 gcatgacgtt tgtccagtcc cgcgacatag cttacggagc gtcccgggac tggaaattca
      781 gttggcaatt gtgggactca gagtgtagtt tctgcttagc caggattcga tagccacgcc
      841 gcagtcctcg ccgctcgcgt agtgaaggta gctgctccac gcccatgttt ccggtcgctc
      901 ggccaatcca cgcttcgtcg gatttgtgat cccaggttag cccaagagaa ggcgaaccct
      961 tcgacaatct aagggcaggc tctggggcac cggctttcct cccgacgatt gcgatggagg
     1021 tacttcagtt tctcggcgaa ctccttatgg ttccactcgt tcctgtctta gtaaagttgt
     1081 tgccagaagg gggccagaag ctgtctcaaa agtgacagac ggacggagat cgaagtcccc
     1141 cggaggaagc gttctagcgg tcgaagcctt cccaccacgt gaagatttcc gcgccgaagg
     1201 cagcggccaa ggccgcgacc tgcgggccgg ggtcaccgta gacttccacg cgggtagtga
     1261 cggccacctg gttgaattgg aatatgttct ggtgtacggc ggggccatca atccagtgcg
     1321 cattgatggc ggcttggtcg gcgtaaccct ggaccagacg gcaacgcttg cgatcggtgc
     1381 tgagcacaaa gtggtaggcc agagtgccgg gttcctgctc gaattcctcg accatttgtt
     1441 tcgcgatggc ctgaaactcg tcggacttgc cgtcgtaaat ctcgaagtcc atcaaaacga
     1501 gaatacgggt ggtgctcatg ggtggctcct tgaaacctga cgggctccca cgccgccgac
     1561 ggagtacgcg gagcaaagcg tgccagccgc actgcgagtc ccacattgtg ggacatcgac
     1621 ccgctactgc ttgtcgtaca cgtacttggc gtggatcttc ttgccgcccg cggcgaagta
     1681 ggtcatttcg agggtgcggc tcttgccatc cttggcgagt tcgatcttgc cattgctcac
     1741 cgtcttgtcg cccttcatgt tggcggggag cagggtgcgc tcgcctttgg ctttgtaagc
     1801 gcggtagtcc acgttaggat cgccggtgac gggataaggt ttagcgtcga acttgccggt
     1861 ccactcggag tgcgccggct ggcctgtggc gttcacgcca tcggtggtga ccttgatgtc
     1921 gctgccctcg gcggaataca ccacggttgt gttcttgccg acgccggccg ggatcctgga
     1981 cttggcttcg ttgagcttcc aggtgcctag gttggggttc tgggcaaagc cgacggtgag
     2041 agccagaagg cacagcacga acgtcactac gatggatctg atcttcatgg agtctccatt
     2101 ggaatcgagc agcagtacgc cgggccagac agaccgcgtc agaatatgcg gagacgcccc
     2161 ggcacgcaag gaaaattgcg gggcaaccga tggagctctg ctcgcctact ctccggtcag
     2221 cgctttgtac tgtggcttgg tgagcggcac aacggaaagc ctgcctatgc gtaccagggg
     2281 cgaatccgcg aacagcttgt tggccttgat ctctgccagc gagacgggct tcggcagcgg
     2341 ctttcctacc ttgatcttca ccaacggttt cttcggatcg gcagcatcaa ccgagacgac
     2401 cgacgcggtt cctaccgcac tcttgtgatc gccagtctca tagataacca gtagcgctcc
     2461 gggctccatt tcgcgcaggt tcctgacagc aacagggttc gttaccccgt cccatgtggt
     2521 cactttgtcg cgctgcaggt cggcgaacga gtactccgaa ggttcagtct tgagcagata
     2581 gtagtcggga tccatgcggt gttcctttcc ggagtatggt cgcataacgg ggagtgcgat
     2641 tccaagtggt gctggacatt cagccaggga cgttcaactc caccgccgca ccttcacccc
     2701 gccactgacg cggtcttgga gcacacctga gaaaaacatg tagggatgcg cagcttcgag
     2761 ttggctggcg gcctccagct tggcgcgcag ctcggcgggg atttcgaagc tcagcgcggc
     2821 aaggttgtcg tcgagctgcg ccaccttggt cgctccgatg atcgttgagg tgacgcccgg
     2881 ttgcgttgcc acccagttca gcgcaacctg cgcggccgga cggttcaacg ccttcgccac
     2941 ttccagcagg gcatccagga tgctaaagtt tcgctcgtta aatttcttga aggcgggatt
     3001 gtcggctttc ttcaatactt cgaggcggct gccctggcta ctctccgcgc ccttttcccg
     3061 ggtgtatttg cccgcgagga aaccactggc cagcgggctc cacgggcaga ctcccatgcc
     3121 caattctcgc gctgcgggaa tgtgttcgcg ctcgatggtg cgttctacca gggagtattc
     3181 cagctgcagc gcgacaattg gctctcgtcc cagacacgcg gccagcgtct ggccgcgggc
     3241 cgcgtaccat gccggcacat cggagaggcc gtagtatcga atcttgccct cgcgcaccag
     3301 gttgctgagc gtatcaacca cctcctcgac tggagtgacc gtgtcccaca catgcatcca
     3361 gtagagatcg agatagtcca tcttgaggcg ccggagcgaa gactcgagcg cgcgatagat
     3421 gttcttgcgc ccgttcccgc ccgagttggg attgccatcc tgcggattga agctgaactt
     3481 gctggcgatc accagcttgt cgcgcagccc gacttcggcc acaaaccggc ctaatatctc
     3541 ttcggatttg ccccccgaat agacatcggc gcagtccatc acgttgccac cattctcgac
     3601 ataacgatcg aaaatgcgac gcgattcggc gtcgtccgat ccccatcggt cgttgccaaa
     3661 cgtcatcggt cccagggaaa gcgggctgac gcgcagtccc gagcggccca gggttacgta
     3721 ctcgttcaat tgcatgatga gttcagtctc tccgtttgaa atatcggatg gttcgatgat
     3781 gtacaagtcg cggaagttgc ggcaaagggt ttgttcttga aactggaaac tcgaaacttg
     3841 aaactgtctt cttactcgta ccgcaggctc tccaccggat cgagttgcgc ggcccgcgcg
     3901 gccggcatgg tgccaaacag cactccgatc agcgaagaca ccagtaccgc gatgacgacc
     3961 gaaaggccgg agatcggaat gcggtagttg gtcagcacac gcacggaaat gggcaacgcc
     4021 aatccaatga ctatgccggc aaaccctccg atcagcgaga tgaggattgc ctcggtaagg
     4081 aattgcgtcc ggatttccgc cttggtggcg cctacggcct tgcgcacgcc aatttcgcgg
     4141 atgcgcgaat tcaccgtggc cagcatgatg ttcatgatgc caacgccgct caccagtagc
     4201 gtgagcaccg ctatcagcag cagtatggtg gtcagcgcat tggcgatctt tcccgcaacc
     4261 gcgatcaaca gtgtcaagtt ttcgaccttg taaaccgact cgggccgatg ccgcgagtga
     4321 agtaccttct gaatttgctc cgtggctccg gggatgtccg aagaatcggc gaccgaaaag
     4381 aacaggctct ttacggtgtc cgtcgtggtg aagaagcgcg ccacggtgta ggggatgagg
     4441 atggtgtcgt cggaaatctc cgactggccg aaggtatcga cgcgctcgcg gaacgtgccc
     4501 acaaccacga agggcaggcc gctgagtttg atgacctggc ccacggcggc gtcggtcgaa
     4561 ccgtacaggc tgttggcgag gttctgggta atcgccgcga ccttgccacg cgtctggctg
     4621 tcgccgtcct ccagaaaacg tccggtgagc acttcgaggt tgcgcaccca caggtactcc
     4681 gccgcaatcc caagcaccag gacatcgcgt tcccggccac ccggcacggc gatgcggtcg
     4741 tgcaactcga ccatcggcga cgacgcggta atgttgggta cctgctggcg gaccgcgttc
     4801 aggtcctcga tcgtcatgta gtcgttggcg gattcggcgc tatagatcga gccgccttcg
     4861 taatcggcag tgatcatgtt ggcgccgatc gactggatct gctgcagcac gtactgcttg
     4921 ccggtgagcc cgatggtcac caccaggatc agcgacgccg tgccaatcac catacccagc
     4981 gcggtgagcg cgaagcgcgc cctgttactg cgaaacgtct caatggcgac actgacagtt
     5041 tctcccaaca ccagcttggg ataacgccgt ccgttcttgc cgttggccgc catgcctatg
     5101 ctggttagac taaccctaac gctgccgcgc ttcaatgtag cggggcactc ggcagacgtc
     5161 tggcaacggc gatgttgcgg ctaccgctgg ggtatgatcg tctttcaggg ttcaatactc
     5221 aggctcaatc acctctgatg tcccagttcc ttcatcgttc cgcactcact caaacgttta
     5281 taggcgcctt gcttttcgcg atcgcagccg gcacggcgct tgcccagcag aacgtgggtg
     5341 aactcttcgc ctccgacgcc agcgtgaagg gttcggtagt cctcgccaat tcaggaacca
     5401 ctgtgatgag cggctcatcc atcgccgctg gagcccaatc cgccactctc aaactcgagc
     5461 gtggtggcag catgataatt tgcccgggca cgaaccttgc gatcaccacc tcgcagaacg
     5521 gccgtcagct catgttcagc atggacaccg gcaacatcga gatggattat cccatcggcg
     5581 ccgctgccga c
//
LOCUS       DASRKK010000788        10874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_966409, whole genome shotgun sequence.
ACCESSION   DASRKK010000788 DASRKK010000000
VERSION     DASRKK010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966409"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="VFC15_18925"
     CDS             complement(<1..1644)
                     /locus_tag="VFC15_18925"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HZM12287.1"
                     /translation="MKTGNSSFRELFGEGQRVEIPVIQRDYAQGREDEHSREVRKHFI
                     EALGTAFSPDRADAAPLDLDFVYGRWRVTDKTLEPLDGQQRLTTLFLLHWYLASLDDV
                     FEEFRSWITLADGGSCFTYRTRPAAREFFDALVLQPAPLNALVSESPTLSNWVTDSVW
                     FVRNWQRDPTVRGCLTMLDAIHGRFASSKGAWPRLVSRESPAIVFRLLLLENFNLSDD
                     LYVKMNARGKALTRFEILKAEIEQFVRKEFGDERCPHEPVIGWRDYISRQFDIAWTDF
                     LWKHRGGTTEIDSRFMHLIRALALVHCVGRGDDADLAEQVERLLVNPEPGVPVYQDLG
                     CLDRAFIEELVVGLDTLAQHEDAPGFLGRSDYLDEKDVFRRVLLARTANQADGLTLVD
                     WVMLYAWCSFLLQFSKDLRCEAVRGSFHDWIRVIVNLAKNSNIDRNERLVAALRGSKQ
                     LSLKCGTDLLSRIANGALDESGGFNQQQQREERLKAQLILRNTAWRPLIERAETHLYF
                     RGDIEFLLRFSGVFDRAAQNGRCDWSDEEDGALRQSFADW"
     gene            complement(1631..3502)
                     /locus_tag="VFC15_18930"
     CDS             complement(1631..3502)
                     /locus_tag="VFC15_18930"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HZM12288.1"
                     /translation="MTTTTPQLRLRSVFDLLNERFFVASYQRGFRWTAQQVEALLEDL
                     AAFQLSSRQSSPDTYYCLQPVVVRRRAESEWELVDGQQRLTTILLILRTLHEIATMLN
                     LGCYEIAYETRDASGEFLKQPTREGAFQSIDHYHIYEAYQTIQRWFNGRDGGLRLRLL
                     DCLTGRDGAAPNVRVIWYELDQHQDPVQTFVRLNVGRIPLTSAELIRALLLRSDRAPL
                     DPRDAQQIPQDWDLIERRLQDDGYWYFLQSRRSPPPTRIEYLFEIFVRLKRNDVTDGA
                     GDDPLATFLAFQALLDAEDAQVWQIWQEFKKLTQTLEDWYDDRPLYHLVGFLVATATD
                     DKTSGGLTRQPEASILVDLVKARMNSTGTGFDRRLRRLAWRRFAGPRGEEPPADGFTK
                     NDLVERILERIEALNYGSDPVGAVLLLFNIASLLDQTASTQRFQFDGYKKNSWDIEHV
                     RSVAEYIPRAAADRRRWLEHARDFVVSPLATGWDPTSSEKLRQDIAALVATPSPEEDI
                     FTQVFDRVRALSGEAGARVDDNALSNLVLLDVGTNRSYKNAIFPVKRTRIIDLDKRGQ
                     FVPPATRNVFLKYYSPQAAQLMLWDGADQDAYGAVIEETLLQFFAPLTRNEGVNEDW"
     gene            complement(3969..4934)
                     /locus_tag="VFC15_18935"
     CDS             complement(3969..4934)
                     /locus_tag="VFC15_18935"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HZM12289.1"
                     /translation="MKSTAHSYRWFGSQWQFQAVTLVAIMLLLGLFNRPSVAQQGNEK
                     TFASPGDAVLALYNAAKSDNSQVLDSIFGSGAKEILNTGDSVADKKMIADFVRRYDLM
                     HRVVIEPDQTATLYVGPENWPTPIPLVKNNSGAWYFDTEAGVKEVLYRRVGTNENDAI
                     EVLHTLVDAQREYGSVVRDGEKTKHYANKFVSDQGKQNGLFWKTDDNQPPSPIGPLLV
                     QAATEGYNVQQGKQTPFHGYYYRMLTAQGAAAKGGARSYLVNGELARGFAFVAYPAEY
                     RNSGVMTFIVNQDGSVHEKDLGPDTEKLASAMSEYNPDPTWVRVE"
     gene            complement(4996..6438)
                     /locus_tag="VFC15_18940"
     CDS             complement(4996..6438)
                     /locus_tag="VFC15_18940"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HZM12290.1"
                     /translation="MPIFKQALSLYLSACVIFSVLGLDGCSKKSEPAQSQAQSGEAQQ
                     AQSGQAPAATYSTPTPDQLYQLVAPIALFPDNLVAMVLAASTYPDQVRDAYQWLQQNA
                     NLKGQQLTDAANQQPWDVSVKGLTQFSDVLSQMATNLSWTSSLGDAYYNIPQSVMNAV
                     QVMRQRAMQSGNLKSTQQQNVTVQNQAPASAPPPTESSEAAPVTVVQPPPQTIIIQPA
                     QPEVVYVPTYNPTVVYGAPVPAYPGYSTGAMVATGVISFGLGIAVGAAINGGGCCGYN
                     SWGCGWNNSTVVYNHNTYISNSNTFANRNVNVNNFNRNNVNANNFNRNNLNANNVNRN
                     NLNRNDVNNNRANNFNGSTPHFNQKYDQPQFRNQGQGNLAQQRGGNQPNFNQQNRAGG
                     GQPNFNQQNRGGMNQNQARAGNLNQNQRQAQRGYGQQAAPGSGKSAFSNYGQGGNTRA
                     NSARGQQSLQGRSGGGVANAQRGGGGGRRR"
     gene            complement(6847..7545)
                     /gene="mtgA"
                     /locus_tag="VFC15_18945"
     CDS             complement(6847..7545)
                     /gene="mtgA"
                     /locus_tag="VFC15_18945"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZM12291.1"
                     /translation="MPDTHAKAPLRTTFLRSFIRWLVIAVVLFWSFAALVLVAARWID
                     PPTTAVHIQRCLQAWIHHTPYRERYKFIPLSQISPDLQHAVIAAEDAHFYQHHGFDWH
                     EIQIAAEDDLEGDRTRGASTVTQQLVKNLFFGTGRSILRKGAEVTLVPVAEFVLGKQR
                     ILELYLNVVEWGRGIYGAESACRYYDETAARNIGRQQAARLAAILPAPLKRRPERMNS
                     YSAIILKRMDQMDW"
     gene            8119..10704
                     /locus_tag="VFC15_18950"
     CDS             8119..10704
                     /locus_tag="VFC15_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM12292.1"
                     /translation="MALAPGTRLGPYEIVAAIGAGGMGEVYRARDTRLDRTVAIKILP
                     ESFANDRERLERFQQEARILSSLNHPNLMAVYDVGVQDGMHFLVSEFLEGETLRDRMN
                     AAPLPQRRVIEYGLQIAKGLAAAHEKGIVHRDLKPENVFVLRDERVKILDFGLAKQRA
                     MAATADATMTSPNQTSAGTVLGTAGYMSPEQVRGEAVDNRSDIFSLGTILYEMISGQR
                     AFRGDSNVEVMTSILKAEPRDLSESGMNVSLGLQRIVHRCLEKKPEARFQSASDLAFA
                     LDSLSNPGSTSTGGLRALKDGKSKVSRLLIPALLLAAAIGALATYFLPPSPVSHPDFT
                     QISFRSAYIRHARFAPDGRTVVYDSTTDGKPTELFSTRTDTIEAQSLHIPASLLSVSS
                     TGELAVTLDTHFDPKYTPTGRLARVPIGGGSTRELLDAVTEADWTPDGSALAVSRQVG
                     HHFQLEFPLGKTLYQNDGYISDVCFSPAGDKIAFVDHSIFGDDRGVVDVVGLHGHRKV
                     LTREFSTVQGLAWAPKGNEIWFTAGLNSEPSSLRAVSLEGKERMLLSSPAKLKLQDVS
                     RDGSVLLSVEDYRDQQILSETTEGKVRDVSSFPFQITEAFSSDGKTLLLNTYVTGGTS
                     DYNLYTQKTDGSAPAMIGQGAGLGFSFDGKWATAVDPLNLQHLRVIPTGVGESRTLSA
                     PSGTQYIGAAWMPDGKHILTLALAPGHTPNNYLQDVETGAAQQIKSEDKYLLVSQAGL
                     GAVSPDSKYFLAVDNEKRYWVQPIDGGAASEIKGLSSGDIPVEWHGGSENIFFERPIG
                     TDTSEIYDLNLATGASKLWSHFTPTDKTAMLALRHAIVTQDGVHVLYVVQRIFSTLFV
                     AKGIQ"
BASE COUNT         2164 a         3144 c         3288 g         2278 t
ORIGIN      
        1 ccagtcggcg aaagactgcc ggagcgcgcc gtcctcttcg tcgctccagt cgcaccgtcc
       61 gttctgcgcc gcgcgatcga agacgccaga gaatcgaagt aggaactcaa tgtcgccccg
      121 gaagtacaga tgcgtctcgg cccgctcgat gagcggccgc caggcggtgt tgcggagaat
      181 gagttgggcc ttgagccgtt cctcgcgctg ctgctgctgg ttgaatccgc cgctctcgtc
      241 aagcgcgccg ttcgcgatgc gcgagaggag gtcggttccg catttgagcg agagctgttt
      301 cgacccacgc aacgcagcga caagcctctc attcctgtcg atgttggagt tcttggcgag
      361 attgactatg acccgtatcc agtcgtggaa cgatccgcgc accgcctcgc accggagatc
      421 cttggagaac tggagaagga acgagcacca cgcgtagagc atcacccaat ctaccagcgt
      481 gaggccgtct gcttggttcg cagttcgtgc gaggagaacc cgccggaaga catctttctc
      541 gtctagataa tcggagcgac cgaggaatcc cggcgcgtcc tcgtgctggg cgagtgtgtc
      601 taggccgacg actagttcct cgatgaaggc tcggtcgaga cagccgaggt cctggtagac
      661 cgggacaccc ggctctgggt tgactagtag ccgttcgacc tgttccgcta ggtccgcgtc
      721 gtcgccccgg cccacgcagt ggacgagcgc gagggcgcgg atcagatgca tgaaccgtga
      781 gtcgatctcg gtcgtgccgc cgcggtgctt ccacaggaag tcggtccatg cgatgtcgaa
      841 ctgccggctg atgtagtccc gccatccgat cacaggttcg tgcgggcatc gctcgtcgcc
      901 gaattctttg cggacgaact gttcgatctc ggcctttaag atctcgaacc gcgtcagcgc
      961 cttgccgcgc gcgttcatct tcacgtagag gtcgtcagat agattaaagt tctcgagtag
     1021 cagcaggcgg aacacaatgg ctggcgactc cctcgacacg agccgcggcc aggcgccctt
     1081 ggatgaggca aagcggccat gaatggcgtc gagcatcgtg aggcagccgc gcaccgtcgg
     1141 atcgcgctgc cagttccgca cgaaccacac cgagtcggtc acccagttcg agagagtggg
     1201 tgattcggat acgagcgcat tcagcggcgc cggctggagg acgagtgcgt cgaagaactc
     1261 gcgagcagcc ggacgcgttc gatatgtgaa gcacgagccg ccgtcggcaa gcgtgatcca
     1321 agaccggaat tcctcgaaga cgtcatcgag actggcgaga taccagtgca gcagaaacag
     1381 cgtcgtcagc cgctgctgcc cgtcgagtgg ctcaagggtc ttatcggtca ccctccaccg
     1441 tccgtagacg aagtcgagat cgagaggcgc ggcgtccgcc cggtctgggg aaaacgccgt
     1501 gccgagcgcc tcgatgaagt gcttacggac ctcccggctg tgttcgtcct cgcgtccctg
     1561 tgcgtagtct cgctggatga ccggaatttc gacgcgttgt ccctcaccga agagctcgcg
     1621 gaagctagag ttaccagtct tcattcacac cttcattgcg cgtgagcggc gcgaagaact
     1681 gtaggagcgt ctcctcgatc accgcgccgt atgcgtcctg atcggctccg tcccagagca
     1741 tcagctgcgc ggcctgcggc gagtagtact tcaagaacac gttgcgcgtg gcgggcggaa
     1801 cgaactggcc ccgcttgtcg aggtcgatga tccgcgttcg tttcaccgga aagatcgcgt
     1861 tcttgtacga ccggttggtg cccacgtcga ggagcaccag gttcgagagc gcattgtcgt
     1921 ccacgcgcgc ccctgcctcg ccgctcagcg cacgcacgcg gtcgaatacc tgcgtgaaga
     1981 tgtcttcttc gggcgacggc gtggcgacga gcgccgctat gtcctgccgg agcttctccg
     2041 acgacgtcgg atcccatcct gtcgcgaggg gactcacaac aaagtcccgc gcgtgctcaa
     2101 gccagcgcct ccggtccgcg gccgcccggg gaatgtactc cgcgacggac cgaacgtgct
     2161 cgatgtccca ggagtttttc ttgtagccgt cgaattggaa ccgctgagtg gatgcggtct
     2221 ggtcgagtag actagcgatg ttgaacagca gtagcacggc cccgactggg tcggacccgt
     2281 agttaagagc ttcgatgcgc tccaagattc gctccaccag atcgttcttc gtgaagccat
     2341 ccgccggcgg ctcctctccg cgcggacccg cgaagcgcct ccacgccagt cgcctgagcc
     2401 gtcgatcgaa cccggtgccc gtggagttca tccgagcctt tacgaggtcg accaagatac
     2461 tggcttctgg ttggcgcgtc agaccgcccg aggtcttgtc atctgtcgct gtggcgacga
     2521 ggaagccgac gaggtggtag agcggccgat catcgtacca atcctcgagg gtctgagtaa
     2581 gcttcttgaa ctcctgccag atctgccaca cctgggcgtc ctccgcgtcg aggagtgctt
     2641 gaaacgccag gaaggtcgcg aggggatcgt ctccggcacc gtccgtgaca tcgttgcgct
     2701 tcagtcgaac gaagatctcg aagaggtact cgatgcgcgt cggaggaggc gatctgcggc
     2761 tctggagaaa gtaccagtac ccgtcatcct gcaggcgccg ctctatgagg tcccagtcct
     2821 gcggtatctg ctgcgcgtcg cgcggatcca gcggcgcgcg atcggagcgc aggagcagtg
     2881 cccggatcag ttcggcgctc gtgagcggaa ttcggccaac gttgaggcgt acgaacgtct
     2941 gcaccggatc ctggtgctgg tcgagctcgt accagatcac gcgcacgttc ggcgcggcgc
     3001 cgtcacgtcc ggttaggcag tcgagcagcc gtagccggag ccccccgtcc cggccattga
     3061 accagcgctg aatggtctgg taggcctcgt agatgtggta gtggtcgatc gattggaacg
     3121 ctccctcccg cgtgggctgc ttcaggaatt ccccgcttgc gtcgcgtgtc tcgtaggcga
     3181 tctcgtagca gcccagattc agcatcgtgg ctatctcatg gagggtgcgc aggatcagta
     3241 agatggtggt cagtcgctgt tgcccgtcaa cgagttccca ttcactttcg gcccgacgcc
     3301 ggacgacgac cggctggagg cagtagtagg tatctggact gctctgtcgt gaggatagct
     3361 ggaatgccgc gagatcttcg agcagcgcct cgacttgctg cgccgtccag cgaaagcccc
     3421 gctggtacga tgcgacgaag aaccgctcgt tcaacaggtc gaagaccgac cgcagtcgaa
     3481 gctggggtgt ggtagtcgtc atcgttatcg gtatcgatcc aacggattat aattcagcga
     3541 cttgaacatt atcgaagtcg aaatccggca ccggcgaatt gttgcgtgcg cggcagattg
     3601 acgtatcacg cccaacctgt gctgttgaag aactccacca ggtcgcggcc acaaggacgg
     3661 aacgccgact aaccggccaa gtggattccc ggacactggg gcgttcctct gaaactaccc
     3721 ccaggccgac ttggataacc tcagacgcgc ctggcacgag ttcagtgttg tcggtgactc
     3781 gggaggaatc tggctccaga gagttcccgc aggtggcgcc acttcctcgc tcgtataaga
     3841 ccgatttttt cattaattga cattccccga cgcgtctcaa tctcccggat ggccaataac
     3901 agaggagtct cctgacctct cgttcattaa gaccaatcag gacccatcga tcgccaccca
     3961 gccgaatctt actcgacgcg gacccaggtt gggtcgggat tgtattccga catggcgctt
     4021 gcgagtttct ccgtgtcagg ccctaggtcc ttctcgtgca ccgacccatc ttgattcacg
     4081 atgaacgtca tgacgcctga attgcggtac tcggcgggat aggcaacgaa ggcgaagccg
     4141 cgagccagtt caccatttac caggtagctg cgcgcgccgc ccttggctgc ggccccctgc
     4201 gcggtgagca tgcggtagta gtaaccgtgg aacggggtct gcttcccctg ttgtacgttg
     4261 tagccttccg tggcagcctg caccagcagc ggaccaatcg ggctcggagg ctggttgtca
     4321 tccgtcttcc agaagaggcc gttctgcttg ccttgatcgc tcacgaactt gttcgcgtag
     4381 tgcttggtct tctcgccgtc gcgcaccacc gaaccgtact cgcgttgcgc atccaccagg
     4441 gtatgcagaa cctcgatggc gtcgttctca ttggtgccga cccggcgata gagcacttcc
     4501 tttacgccag cctcggtgtc gaagtaccac gctccactgt tgttctttac gagcgggatg
     4561 ggcgtgggcc agttctccgg ccccacgtag agcgttgccg tctggtcggg ctcgatgacg
     4621 acgcgatgca tgaggtcgta gcggcggacg aagtcggcga tcatcttctt atcggcaaca
     4681 ctgtcaccgg tgttgagtat ttcctttgcg ccggaaccga aaatgctatc cagcacctgg
     4741 ctgttatcgg acttggcagc gttatagaga gcgagcacgg catcgccggg cgaagcgaac
     4801 gtcttctcat tgccctgttg ggcaacggaa ggccggttaa acaacccgag caatagcatg
     4861 atcgcaacca gagtgaccgc ttggaattgc cactgggaac caaaccaccg gtaagaatgc
     4921 gctgtagatt tcatggtcct tgccttcctg cccttccaat aagatcgata ccaacccaag
     4981 ccttactcgt aggagttacc ggcggcgtcc gccgcccccg ccgcgttgcg cattagcaac
     5041 tccgccgcca ctgcggcctt gcaggctctg ttgcccacga gcgctgttcg cgcgagtgtt
     5101 tccgccctgg ccataattgc tgaaggcgct cttgccggag cctggggccg cctgttgccc
     5161 gtagccccgt tgcgcctggc gctgattttg attgagattg ccggcgcggg cctggttctg
     5221 gttcattccc ccgcggttct gctggttgaa attgggttga ccgccgccgg cccggttctg
     5281 ctgattgaag ttaggttgat tgcccccgcg ttgctgcgcc aggttcccct ggccttgatt
     5341 tctaaactgc ggctgatcgt atttctgatt gaaatgcggt gtgctgccgt tgaaattgtt
     5401 ggcccggtta ttgttgacgt cgttccggtt caagttgttg cggttaacgt tgttcgcatt
     5461 caaattgttg cggttgaaat tgttggcgtt gacgttattc cggttaaagt tattcacgtt
     5521 tacattgcga ttcgcgaagg tattggagtt ggaaatgtag gtgttgtggt tatagaccac
     5581 agtggagttg ttccagccgc atccccatga gttgtaaccg caacatcccc cgccattgat
     5641 ggccgcacct accgctatgc ccagtccaaa cgagatgacg ccggtagcta ccatggcgcc
     5701 ggtcgagtat ccagggtacg cgggcacagg cgcgccgtaa acaaccgtcg gattataggt
     5761 aggcacatac accacttccg gttgcgccgg ctggatgata atggtctgcg gcggaggctg
     5821 cactacggtc accggcgctg cctctgagga ctcggttggt ggcggcgccg aggcaggagc
     5881 ctggttttgt acggtgacgt tctgctgctg ggtgctcttc agatttcccg actgcatggc
     5941 gcgctgccgc atgacctgca cggcgttcat cacgctctgc gggatgttgt agtacgcgtc
     6001 gcccagagaa gaggtccagg acagattcgt ggccatctgg ctcaatacat cagaaaactg
     6061 ggtcaggcct ttcacgctta cgtcccaagg ctgttgattg gctgcgtcgg tgagctgttg
     6121 gcccttgagg ttggcattct gctgcagcca ttggtaggca tctctgacct ggtccggata
     6181 agtggacgct gcaagcacca tcgcaaccaa gttgtcggga aagagtgcga tgggagcaac
     6241 caactggtag agctggtccg gcgttggcgt ggaataggtc gcggcggggg cctgccccga
     6301 ctgcgcttgc tgagcttcgc cagattgcgc ctgagactgc gctggctccg acttcttgct
     6361 gcagccgtcc aaccccagga cactgaagat cacacaggcg ctgagataca gcgagagagc
     6421 ttgcttaaaa attggcataa cgaggcctcg tgaatagaaa gaacagccaa atagcacagt
     6481 ggaatcctac acgcacggcg agcgctatgc acgcagttgt acagtcgctg attgtcatgt
     6541 cttacgaaaa acattcctgt gaccgaaatc cccatctagc cgcgtagcat tcctggctgg
     6601 gagacccaaa taggatttaa ggacatgaga gttggtccga tgagttggca tctcgtcagt
     6661 aaatcaagcg cggacgagat gggcgtttag cgataccatt ctccaaagta gctcaggaat
     6721 ggtgccgcat cggttcctgc catatgcttt gttgtggaga tagtaatttc cgaatcggcg
     6781 accattcgac cagaatccag cgcgagagta ataaaagccg ccgcattggt aaagtattag
     6841 ccacgattac caatccattt gatccatccg cttaaggata atcgcgctgt agctattcat
     6901 gcgctcgggg cgtcgcttca ggggagccgg cagaattgca gcgagccgtg ccgcctgttg
     6961 ccggccgata ttccgggctg cggtttcgtc gtagtaacga caggccgact ctgcaccata
     7021 aataccacgg ccccattcga ccacgttgag gtatagctcc agaatgcgct gtttgccgag
     7081 gacgaattcg gccaccggta ccagtgtgac ctctgcgccc ttgcggagga tagagcggcc
     7141 cgttccgaag aacaggtttt ttacgagttg ctgagtaacg gtggaagctc cgcgagtgcg
     7201 atcgccttcc aaatcgtctt cggcagcgat ttgaatctcg tgccaatcga atccatggtg
     7261 ctggtagaag tgcgcgtcct ctgcggcgat tacagcgtgc tggagatcgg gcgagatttg
     7321 gctgagcgga atgaatttgt agcgttcgcg atacggcgtg tggtgaatcc aggcctgcaa
     7381 gcagcgctgg atatgcaccg cggttgtcgg tgggtcgatc caccgggcgg ccaccaggac
     7441 cagcgcggca aacgaccaaa aaagcaccac ggcgatgaca agccatcgaa taaatgatcg
     7501 gaggaacgtc gtccgaagcg gggccttggc gtgtgtgtcc ggcattgcag tgacatttca
     7561 ggataatggg ctgggctgcg ttgccgccga tggatcatct tgcgcgcgaa cacagagtac
     7621 cactgaggaa agccccttga ttgtgaatca cattgaaccc cgctgcaaag gtaaggtggg
     7681 atcagtcccg acagcagtac gtaaatggct cgaacggcgt ttatcgttac tgatctgcag
     7741 gaagatcgat cgttttcata attcgctttg ggtaaccttt tgatctagat ctagatggac
     7801 cgagttggaa caacggacgc tgtaccgagc taacatgcca cccccaccat cggatgaggg
     7861 tgtctagcga cgcataggct gctgcggggg ccgtcaacaa cctcagagga gaccgctaat
     7921 gcaatccctc aatgaccaag caagtccaca cttcacggct tatatcgtcg ataagttggc
     7981 cggcctaggc ggttaatgtg atgcgacgaa tccccacata atcctggatt agagatactg
     8041 tgcctccatg gacaaacctc cgcgtttcca gatttggcta ctcacggtag aatcagccat
     8101 cttcctttca ggtgaaaaat ggctctggcc ccgggaaccc gcttaggccc ttacgaaatc
     8161 gttgccgcaa taggggcggg gggaatgggc gaagtctatc gcgcccgaga tactcgcctc
     8221 gatcgaacgg tagccatcaa gatccttcct gaatcgtttg ccaacgatcg cgaacgcctg
     8281 gagcgcttcc agcaggaagc ccgcatcctg agctcgctca atcaccccaa cctgatggcg
     8341 gtctacgacg tcggcgtgca ggacggcatg cacttcctag tgtcggagtt tcttgagggc
     8401 gaaacactgc gtgatcgaat gaacgcagcg ccgctgccgc agcgccgcgt catcgagtac
     8461 ggattgcaga tcgccaaggg gctggctgcc gcgcacgaga aaggcattgt ccatcgcgat
     8521 ctgaagccgg aaaacgtctt tgtgctgcgt gacgaacgcg taaaaattct cgatttcgga
     8581 ttggccaagc agcgagcgat ggcagcgacc gccgatgcga ccatgaccag cccgaatcaa
     8641 acttccgcag gcaccgtgct cgggacggca ggttacatgt cacccgagca ggtgcgcggc
     8701 gaagcggtcg ataaccgttc cgacattttc agcctgggca ccattctcta cgagatgatc
     8761 tccgggcaac gcgctttccg cggcgactcg aatgttgagg tgatgacgtc gattctgaag
     8821 gcggagccgc gggacctgag cgagagcggt atgaatgtgt cgctcgggct gcaacggatt
     8881 gtgcatcgct gcttggagaa aaagccggaa gcgcgttttc aatcggctag cgacctggct
     8941 ttcgccctcg attcgctaag caaccccgga agcacctcga ctggagggtt gcgcgcgctg
     9001 aaggatggca agagcaaggt ctcgcgattg ctgatacccg cgttgctgct ggcggcggcg
     9061 attggcgcgc ttgcgactta ttttcttccg ccttcgccgg tctcgcatcc ggatttcacg
     9121 cagatcagct ttcgctccgc ctacattcgg catgcgcgtt tcgcgcccga cggccgcacc
     9181 gtcgtgtacg actccactac cgacggcaag ccgaccgaac ttttctccac gcgcacggac
     9241 accattgaag cgcagtcgct ccatattccg gcaagccttt tgagtgtttc gtcgaccgga
     9301 gagttggcag tgacgctgga tacccacttt gaccctaagt acactcccac cggacgtctg
     9361 gcgcgcgtgc caattggggg cggttccacg cgcgagttgc tggacgcggt gacggaggcc
     9421 gactggacgc ccgacggttc ggcgctggcg gtgagccgcc aggtcggaca ccatttccag
     9481 ctcgaatttc cgctgggtaa gacgttgtac cagaacgatg gctacatcag cgacgtgtgc
     9541 ttttcgccgg caggagacaa aatcgcgttc gtagaccatt caattttcgg cgacgatcgc
     9601 ggtgtcgttg acgttgtcgg cctccacgga catcggaagg tgctgacgcg ggagttcagc
     9661 accgtgcagg gactggcgtg ggcgccaaag ggaaacgaga tctggttcac agccggactc
     9721 aacagtgagc cgagttcgct gcgtgccgtc agtcttgaag gaaaggaacg gatgctgctt
     9781 tcctcgcccg ccaagctgaa gttacaagat gtatctaggg acgggagcgt tctgttgagc
     9841 gtggaagatt atcgcgatca gcagatcctg tccgagacca cagagggaaa ggtgcgggat
     9901 gtgagttcgt tcccgtttca gattaccgag gcgttttcgt ccgacggaaa gacattgctc
     9961 ttgaacacct acgtaaccgg tgggacttcc gactacaacc tctatacgca gaagaccgac
    10021 ggctccgcgc ccgcgatgat cggtcagggt gcgggactgg gcttttcctt tgacggcaaa
    10081 tgggccacgg cggtggatcc gctcaacctg cagcacctga gggtgatccc caccggcgtg
    10141 ggggagtccc gcaccctaag cgctccgtcc ggaacgcaat atatcggcgc tgcatggatg
    10201 cccgacggca agcacatcct gacattagcg cttgctcccg gacatacgcc aaacaattat
    10261 cttcaagacg tggagacggg tgcggcgcaa cagatcaaga gcgaagacaa gtatcttttg
    10321 gtcagccagg caggactggg ggcggtctcg cccgatagca agtacttcct cgctgtcgat
    10381 aacgaaaagc gatattgggt gcagcctatc gatggaggcg cggccagcga aattaaggga
    10441 ttatcgtcag gcgacattcc ggtggagtgg catggcggtt cggagaacat tttcttcgaa
    10501 cgacccatcg gaaccgacac cagcgagatc tatgacctga acctcgccac cggcgcgagt
    10561 aaactttggt cacacttcac accgaccgac aagaccgcga tgcttgcatt gcgtcacgcg
    10621 atcgtcaccc aggacggcgt acatgtgctg tacgtggtgc agcgcatctt ctcgaccctg
    10681 ttcgtcgcga agggcattca gtagaggcag gttcgttcga cgccctgcgc ggtagttctg
    10741 tgatgaagac tcccaggtct cgaaaaccgc acggccaggg gcaacacggt accttcagga
    10801 cgtgcccgag aagaaccgcc gatttatgat gacgggattt ccgggaggta acgagagctc
    10861 ctcacgacga gatt
//
LOCUS       DASRKK010000789         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_966461, whole genome shotgun sequence.
ACCESSION   DASRKK010000789 DASRKK010000000
VERSION     DASRKK010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966461"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1580
                     /locus_tag="VFC15_18955"
     CDS             240..1580
                     /locus_tag="VFC15_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12293.1"
                     /translation="MPRRRYWLRFPYPAVVGAAILLFSSAHSAPLPPKPNTEPTFYKD
                     ILPILQDHCQVCHRPGGIAPMPLVTYQQAKGSGMAIKQAVVSRQMPPWFADPRCGKFS
                     NDPSLTPQQIELISRWVDSGAPAGSPNDAPSKQWDAGWNIPAPQKIVEMPVPVAIPAH
                     ADVEYTYEIVPTGFTEDKWVQLSQVLPSSPQYVHHAVVYVRPPSSTWLRAAPVGVPFT
                     PSSFSDPKLRAEAHETTTDMLLVYAPGSEPDRWPDGMAKFIPAGSDLVFQMHYTANGK
                     AGTDQTRVGMVFAKQPPKQRVLTLQLTNHTFIIPPRTDDYRVEVQGTMPNDATLLSFF
                     PHMHLRGKRFEYNILHSDGSVETLLRVNYHFHWQLSYRLAEPRFLKAGTRLQAVAWYD
                     NSEDNPYNPDPNKSVMWGGQTYEEMMVGFFDVAVPANVDKEQFFVRHAGKNSAE"
     gene            1709..2212
                     /locus_tag="VFC15_18960"
     CDS             1709..2212
                     /locus_tag="VFC15_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12294.1"
                     /translation="MTVRLIGVTLVIESLCILGFSQITTHPAHRSSANPAAQSKASGT
                     KSQYLEVSFDPSATHLTPQFKGHDIVAISDSVRQAPALAPKSEFETTAAYEARRASFP
                     TQRLIGDINSDSHLAFVVDEGLLNSTSEVFRYEADSGALTATLRGRSQKFFLERNQPD
                     MQTVTIR"
     gene            2271..2930
                     /locus_tag="VFC15_18965"
     CDS             2271..2930
                     /locus_tag="VFC15_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12295.1"
                     /translation="MYVESYGLSFSSPSWVFESSYGSSKVIAMAPELASKIKADLRFL
                     IVCRLKEPWYLHSATGHDATFDDPIKLTEGLNFLQVEPEQIWIVDGRTGEVIRKFSQA
                     LVEAEKAEQLKAQLREFPLIVELSASNAVVPLKVRSDNGNEQFIAVAKNSQILRARTQ
                     VTLIWSVPSDISDLTVTVNGKPYKAEWRTKEKIIGSRRFVDEATVTIRAQDVAEKSSD
                     H"
     gene            2941..3930
                     /gene="mutY"
                     /locus_tag="VFC15_18970"
     CDS             2941..3930
                     /gene="mutY"
                     /locus_tag="VFC15_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZM12296.1"
                     /translation="MLRRALLAWFDANRRDLPWRRDADPYRVWVSEIMLQQTRVAAVL
                     DHYARFLQRFPTVQALAAAREQSVLALWSGLGYYHRARRLHQAAKLIARERHGEFPRT
                     VEAWRELPGIGRYTAAAIASIAFREAVAVVDGNVERVLERMFGEAKGRESAWHQAEAL
                     LDPTRPGDFNQAMMELGAILCTPRAPQCLLCPLHTWCQSRGAAVPNPQPPRKRKQLHY
                     ALARNGDSVLLVQRPSDAARMAAMWELPETHSAKSGNQVLAKFRHSITDTDYEVSVVH
                     APAALVRALNGNARWVTRKQLQKLALTGLARKVLRKLVAESPIRGMESNKDVK"
     gene            3947..4447
                     /locus_tag="VFC15_18975"
     CDS             3947..4447
                     /locus_tag="VFC15_18975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZM12297.1"
                     /translation="MAALEAGIVAPDFSLRTLEGHQVSLQHLLHRGPVVLAFFKVSCP
                     VCQYAFPLYERMAQANRDASISVLGISQNGPSETADFRHEYGVNFPIAIDDADNHYAV
                     SSAYGLTNVPTLFYIAPNGEIEISSVGWSKRDLDEVNAKLSAVRGHEPSLLWRDGEAI
                     QDFRAG"
BASE COUNT         1014 a         1329 c         1339 g          968 t
ORIGIN      
        1 ggcgccacgc ggttcaagaa gaagagttgc cggaacacaa tcagccgagc tagagaggtt
       61 ccagaatcaa tgtacctgcg tgcgcagttg ttcggaccct gagaccgggt gtgagtcctc
      121 gatttgaatt gccaggctgg attgaactgc gagcaatcca gcccgcaggg cggaacaaca
      181 ttagcccagg gcgtaagccc tgggtgaggt tctggaattg cgataggact gagacgcact
      241 tgccgcgccg tcgctattgg ttgagatttc cctaccccgc cgttgtcggc gccgccatcc
      301 tgctgttttc ttcggcccac agcgcgccgc tcccgccgaa gccaaatacc gagccgacct
      361 tctacaaaga cattttgccg atcctgcaag accactgcca ggtgtgtcat cgccccggag
      421 ggatagctcc catgccgctg gtgacgtatc aacaggcgaa gggttcgggc atggcgatca
      481 aacaagctgt ggtttccagg cagatgccgc cgtggtttgc cgatccacgc tgcgggaaat
      541 tctccaacga tccatcgctc acaccgcaac aaatcgagct gatctcgcgc tgggtggata
      601 gcggagcgcc cgccggttcc ccgaatgacg caccgtcaaa gcagtgggac gcaggatgga
      661 acattccggc gccgcaaaaa attgtggaga tgccggttcc ggtcgccatc cccgcgcatg
      721 ccgatgtgga gtacacctac gaaatcgttc ccacgggatt caccgaagac aagtgggtgc
      781 aactctctca ggttctgccg tcgagtccgc agtatgtcca tcacgccgtg gtgtacgtgc
      841 ggccgccaag ttccacgtgg ttgcgagccg cgccggtagg agttcctttc acaccgtcaa
      901 gtttttccga tcctaaattg cgcgctgagg cccacgaaac caccaccgac atgctgctgg
      961 tttatgcgcc gggaagtgag cctgatcgct ggcccgatgg aatggcgaag ttcatccccg
     1021 ccggctctga cttggttttc cagatgcact acacggccaa cggcaaagca ggaaccgacc
     1081 agacgagggt cggcatggtg ttcgccaagc agccgccgaa acagcgcgtg ttgacgttgc
     1141 agctcaccaa ccataccttc attattccgc cgcgcaccga cgactatcgc gtcgaagtgc
     1201 agggtacgat gcccaacgac gccacgttgc tgagtttctt cccacacatg cacctgcggg
     1261 gcaagcgctt cgagtacaac atccttcact cggacggaag cgtcgaaacg ctgctgcgag
     1321 tgaactatca ctttcactgg cagctcagct atcgtctggc cgagccgcgc tttctaaagg
     1381 ccggaacgcg attgcaggcg gtggcttggt atgacaattc cgaggacaac ccgtacaatc
     1441 ccgatcccaa caagtcagtg atgtggggtg gccagaccta tgaagagatg atggtcggct
     1501 tcttcgacgt ggctgtgccg gcgaatgtgg ataaggaaca gttcttcgtg cgacatgccg
     1561 gaaagaactc cgcggagtga cgcggcgagt caaattctgt gcaccctaca ggtcacagac
     1621 ataaacgagt gggggattta acatgcgacg tatctgaggg aagcgtttcg agtgttccgc
     1681 tcagtgaaat accaaagagg ctgcttcgat gactgtgcgt ctgatcggtg tcactcttgt
     1741 aattgagagt ctctgcatcc ttggtttttc ccaaattacc acgcatcccg cccatcgctc
     1801 gagtgcgaat ccagcagcgc aatcgaaggc gtccggcaca aagtcacagt atctagaggt
     1861 atcgttcgac ccttctgcaa cgcatctgac gcctcaattc aaggggcacg acatcgtcgc
     1921 aattagcgat tccgtcaggc aagcccctgc cctagcgccc aagtcagaat tcgagacgac
     1981 cgccgcctat gaggcgcgta gggcctcatt cccgacgcaa agactgatcg gtgacattaa
     2041 ttcagacagc catctggcat tcgttgttga cgaggggctg ctgaatagta ctagcgaagt
     2101 gttccggtat gaggccgact caggtgctct aacagcaacc ctgaggggcc gcagtcaaaa
     2161 attctttctt gagcgcaatc agcccgatat gcagactgtg accatccgtt gagagcagtg
     2221 aaaaatatac ggcgtcgaat gcctttggtg ccaaggtcga agtcacagat atgtacgtcg
     2281 agagctacgg gctgtcattt tcgtccccat cttgggtttt tgaaagcagt tatggttctt
     2341 cgaaggtcat tgccatggca cccgaactcg ccagcaagat taaagccgac cttagattcc
     2401 tgatcgtttg caggctgaag gaaccttggt atttgcactc tgctaccgga cacgacgcca
     2461 cattcgacga tcctattaag ctgactgaag gcttgaactt cctgcaagtt gaacctgagc
     2521 agatttggat tgtggatggt cggaccgggg aagtaatcag aaagttcagc caggcgctcg
     2581 tcgaagccga gaaggcggaa caactgaaag cacaactgcg tgagttccca ctaatagttg
     2641 agctgtctgc aagtaacgca gtagtgccac tcaaggtgcg atcggacaat ggtaacgagc
     2701 aattcattgc tgttgctaag aacagccaaa tcctgcgtgc gaggacgcaa gtcaccctca
     2761 tatggagtgt gcctagcgat atttcagacc tgacggttac tgtcaatggc aagccgtata
     2821 aggcagaatg gcggactaag gagaagatca tcggaagccg tcgtttcgtt gatgaggcga
     2881 cggtgactat cagagcgcag gatgtcgccg aaaagagttc ggatcattaa agagttgagt
     2941 gtgctgcgtc gtgccctcct tgcctggttc gacgcgaatc gtcgcgacct gccatggcgc
     3001 cgcgatgccg atccctatcg cgtttgggtg tccgagatca tgctgcagca gacccgcgta
     3061 gccgccgtgc tcgaccacta cgcgcgtttc ctgcagcgtt tcccaaccgt gcaggcattg
     3121 gccgccgccc gcgaacagtc agtgctggca ttgtggagcg gactcggcta ctaccatcgc
     3181 gcccgccggc tgcatcaggc tgccaagctc atcgcccgcg aacgccatgg agaatttccc
     3241 cgcaccgtag aagcgtggcg tgagttgccc ggcatcggac gatacaccgc ggcggctatc
     3301 gcgagcattg catttaggga agccgttgct gtggtcgatg gcaacgtaga gcgcgtgctc
     3361 gagcgcatgt ttggcgaagc caagggcagg gaatccgcat ggcaccaagc cgaagcttta
     3421 ctcgatccca cccgtcccgg cgacttcaac caggccatga tggagttagg agcgatcctc
     3481 tgcactcctc gcgctccaca atgcttgctg tgtccgctgc acacatggtg ccagtcgcgg
     3541 ggcgccgccg tacctaaccc gcagcctccc cgcaagcgca agcaacttca ttacgccttg
     3601 gcgcgcaacg gagattcggt gcttttggtg cagcgcccgt ccgacgcagc gcgcatggcg
     3661 gcgatgtggg aactacctga gacccattca gctaaatccg gtaaccaggt gctggcgaag
     3721 tttcgtcact ccatcaccga taccgattac gaggtgtcgg tggtgcatgc tcccgccgct
     3781 ctcgtccgcg cactcaacgg caatgcgcgt tgggtcaccc gcaagcaatt gcagaagctg
     3841 gcgctcaccg gactggcgcg taaagtactg cgcaaactgg tggctgagtc cccgatacgt
     3901 ggaatggagt ctaataagga cgtgaagtaa tgggaggtct tgccgaatgg ctgcattaga
     3961 agcgggaatc gtagcgccgg acttctctct gcgcacgctt gagggccacc aggtttcgct
     4021 gcaacatttg ctgcaccgag ggcctgtggt gctggcgttc ttcaaggtca gttgcccagt
     4081 gtgccagtat gcgtttccgc tgtacgagcg catggctcag gcgaataggg acgcgagtat
     4141 cagcgtactt ggcatctctc aaaacggccc ctccgagact gccgacttcc gtcacgaata
     4201 cggcgtcaat tttcccatcg ccatcgacga cgccgacaat cactacgccg tctccagcgc
     4261 ctacggtttg accaatgtgc ccacgctttt ctacatcgcg ccgaatggcg agatcgaaat
     4321 atcgtcggtc ggatggtcga agcgtgacct ggatgaggtg aacgccaaac tctccgccgt
     4381 ccgcggccat gagccgtccc tgctgtggcg ggatggcgaa gccattcagg acttccgagc
     4441 gggttgaagc tcgaagaatt gatccgcggt ctgcggatca agcgcctctt ccagcctcgc
     4501 tccaggacca gtcttttcca attgaagaat gagcctgaag taagcccgag gggatggcgg
     4561 aggaaagctc ctttcctgat ttgtattgaa cgagtctagc agccgtcggg gcggtgggaa
     4621 tgtgggaatc gcgcagcgat ttccaaggtg
//
LOCUS       DASRKK010000790         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_477979, whole genome shotgun sequence.
ACCESSION   DASRKK010000790 DASRKK010000000
VERSION     DASRKK010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477979"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1604)
                     /locus_tag="VFC15_18980"
     CDS             complement(222..1604)
                     /locus_tag="VFC15_18980"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM12298.1"
                     /translation="MKTTTITIAGAGLVGPLLAISLRQRGFPVVLYERRADMRRVQVS
                     DGRSINLAVSARGIYALQQAGVWSELRDIAIPMRGRLMHLLTGELIFQPYGQDESEVI
                     NSVSRAALNIALLNAAEAAGVAIHFQQRCLGCARADDGIVVRLRDEQSGKQTAIAAEV
                     VIGADGSASAVRGEMSRSPEFRLSQERLDYGYKELTIPAGQGGSHAMETRALHIWPRG
                     SYMLIALPNVDGTYGCILFLPFESERGFAALDSKSRVVEFFRANFPDALALMPNLAEN
                     YFANPTGAMVTVRCFPWHQEGNILLLGDAAHAIVPFFGQGINCGFEDCTCFLELLDAA
                     RTSGAESIDWQKLFTDFESSRKPNTEAIADLALENFVEMRDRVADPHFLFKKKVDLVL
                     EARFPGLYVPKYAMVAFHRIPYAVAQQRGRIQDRLLDELCAGINRLEDIDWSKAAMLV
                     HRDLTQLGKR"
     gene            complement(1601..>2133)
                     /locus_tag="VFC15_18985"
     CDS             complement(1601..>2133)
                     /locus_tag="VFC15_18985"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZM12299.1"
                     /translation="LETMLPTHFLAFRGKLDAASGFQSEQFREIEFLCGQKDEKLLHH
                     HAPDLEAYAKLMRRLREPSLHDVFFEALRTIGGPPGTSGSNEGDGHEARVQAIRHLYQ
                     SERQHRDWIDVSERLLEFDELFMGWRLRHIQLVERMIGTRTGTGGYTGSLYLRQTLDK
                     KFFPELWEARTILQAE"
BASE COUNT          447 a          658 c          606 g          422 t
ORIGIN      
        1 atttagtcga tccggagtcg gcagcatggg ccacggggct ggaatgcgtc atacacatga
       61 tccaagacga agctgcatga tgccgccatg ggtgtgacgt atggcccttt tcccaccact
      121 cacggtgagc tattggtctg cggtctcttc gcgattccgc actactcgag cccgaccgca
      181 gtctgaaagc actccgcaac cggcttcaaa cgtggcctgc attatcgctt ccccagctgc
      241 gtcaagtcac gatgcacgag catggccgct ttgctccaat cgatgtcttc caggcggtta
      301 atgccggcgc agagctcgtc cagcaaacga tcctgaatcc gcccgcgttg ttgtgcgacc
      361 gcatacggaa tgcgatggaa cgcgaccatc gcatacttgg gcacgtagag gccgggaaat
      421 ctggcttcca ataccaggtc cactttcttc ttgaaaagga aatgcggatc agccacgcgg
      481 tcccgcattt cgacaaagtt ctccaacgcc aggtcggcga tggcttcggt attgggcttg
      541 cgcgagctct cgaagtcagt gaacagcttc tgccagtcga tcgactctgc gccgctcgtc
      601 cgcgcggcat ccagtaattc caggaagcaa gtgcaatcct cgaacccgca gttaattccc
      661 tgcccgaaaa acgggacaat cgcgtgcgcg gcgtcgccaa ggagcaatat gtttccttcc
      721 tgatgccacg gaaagcagcg gacggtaacc atggcgcccg tgggattggc aaagtagttc
      781 tccgccagat tcggcattaa ggcgagcgcg tcgggaaagt tggcacggaa aaattccacc
      841 accctcgact tggaatcgag ggccgcaaat ccacgttcac tctcgaacgg caggaacaga
      901 atgcagccat acgtcccgtc cacattcggc aatgcgatta gcatgtacga gccgcgcggc
      961 cagatgtgaa gcgcgcgcgt ctccattgcg tggctgccgc cttgcccggc gggaattgtc
     1021 aactctttgt agccgtaatc cagcctctcc tgcgagagcc tgaactccgg cgacctggac
     1081 atttccccgc gaacagccga agccgaacca tcagcgccga ttacgacttc ggcggcaatc
     1141 gccgtttgct tgcccgattg ttcgtcacgc aagcgcacaa caatgccatc gtctgcgcga
     1201 gcgcatccga ggcagcgttg ctggaagtga attgcgaccc ccgcggcttc ggcggcatta
     1261 agtagcgcga tattcaaggc cgcgcgagaa acggaattga taacctcgga ttcgtcttgc
     1321 ccgtaaggtt gaaagatcaa ttcgccggtg agcaaatgca tcaggcggcc gcgcatggga
     1381 attgcaatgt cgcgcaactc gctccacacg ccggcttgct gcagcgcata gatgccgcgc
     1441 gcggaaacgg ccagattgat cgagcgcccg tcgctgactt ggacgcggcg catgtccgcg
     1501 cgccgttcgt agaggaccac cgggaacccc cgctgtctaa gcgagatggc aagcaacggc
     1561 cccaccagtc cagcgccggc gattgtgatt gtggtagtct tcattccgcc tgcaggatcg
     1621 tgcgcgcttc ccatagctca ggaaagaact tcttatcgag agtctgccgc agatacaggg
     1681 agccggtgta gccgccggtg cccgtacgcg tcccgatcat ccgctccacc agttgtatat
     1741 ggcgtaaccg ccagcccatg aacagctcat cgaattcgag cagccgctcg cttacgtcga
     1801 tccaatcgcg atgctggcgc tcggattggt acagatggcg aatggcctga acacgcgcct
     1861 cgtgcccatc tccttcgttg cttccgctcg tgccaggcgg cccaccgatg gtccgcagcg
     1921 cctcgaagaa tacgtcgtgc aatgaaggtt cgcgcagacg ccgcatcagc ttcgcatagg
     1981 cctccagatc gggagcatgg tggtgcagta gcttctcgtc tttctggccg caaaggaact
     2041 cgatttcgcg aaactgttcc gactggaatc cgctggccgc atccagcttg ccgcgaaaag
     2101 cgaggaagtg cgtgggcagc atagtctcca gaa
//
LOCUS       DASRKK010000791         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1162518, whole genome shotgun sequence.
ACCESSION   DASRKK010000791 DASRKK010000000
VERSION     DASRKK010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162518"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFC15_18990"
     CDS             complement(<1..518)
                     /locus_tag="VFC15_18990"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZM12300.1"
                     /translation="MVRISQARLADLQTGKRSPEIDVTRAQLMQTLAEKKKSIDILKS
                     YESQYAAGGVSLTDLIAARAAVETNTALVRQVESELVVAALPARDEQLKAQAEQVAAD
                     RAALEQATWKLQQKEIVSPRDGLVFDTLYREGEWVAAGNPVVQVLPPENLEVRFFVPE
                     PLVGKLKLEQGV"
     gene            complement(546..779)
                     /locus_tag="VFC15_18995"
     CDS             complement(546..779)
                     /locus_tag="VFC15_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12301.1"
                     /translation="MRRLQIKPCFPLLGLAVGLALIMPGCSSKPSNSYEGYVEGKFVY
                     VASPQGGRLTHLSVSRGETVAVSHPLFSLDREP"
     gene            complement(794..1465)
                     /locus_tag="VFC15_19000"
     CDS             complement(794..1465)
                     /locus_tag="VFC15_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM12302.1"
                     /translation="MQKSDKPGRPAASSDRDARELLITAATELFAEHGVAATSFSIIA
                     RRAGLTSAMVHYYFADREQLLDAVVEERLCPFIDYVWGPVTPGDDPTELIAGVVTRLL
                     GSIEQAPWIPSTWMREILNEGGLLRGRALRRIPFDKVRIVAEAIREGQASKAWNPDLN
                     PLLVVFSMLGLVMLHMATIKIWAEIFHRRPMNRKAVGRHITGLILDGLRHSPTPSPKK
                     SRKKR"
     gene            2257..2862
                     /locus_tag="VFC15_19005"
     CDS             2257..2862
                     /locus_tag="VFC15_19005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM12303.1"
                     /translation="MKSAKMVSVLFLLLAVALSLPAVAGTVNMQFNGTPTGNNYSGVP
                     SYPYDLSVNGAPNQWMMCMGYYEHISGGETWQATAVSVGSLDPTSHLPNYEAAFLFEK
                     AVADHGANSDVNAAVWWIFEGAPSLDPGAQALVTLAQSQTYTQGEFSNVMLYTAIPGT
                     ESGNLGTAQDFMSSTPEPGTLALLGSGMIGAAGFLRRKLLP"
     gene            3135..3791
                     /locus_tag="VFC15_19010"
     CDS             3135..3791
                     /locus_tag="VFC15_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM12304.1"
                     /translation="MADSNQMGCRLLSEALDKLPGLAVVASSSNGESLLQAVQAFKPD
                     IAVISATLKDGPFSRRASIPDIRSRTPQCPWILMLDQSEPQLVVAAFRAGAKGVFSRA
                     QTDISLLAKCIRKVMEGEIWVDNKQILCLLEAVTGSDQQSHDQSSSPVKLTPREQSVV
                     RLVVQGMVNREIADALHLSEHTIKNYLFRIFDKLGVSNRVELALYAVAKLDLADSPAA
                     "
     gene            complement(4000..>4987)
                     /locus_tag="VFC15_19015"
     CDS             complement(4000..>4987)
                     /locus_tag="VFC15_19015"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HZM12305.1"
                     /translation="RLTAIARPVFLNAGTPDGTSLYRFGTSLVGTRFGSQSAGGLGGE
                     VQLSTQDFGLRFGISPSGFLVTNFVGGVRWRIAGGPVTLWATRDNVKDTLLSYAGSRD
                     PGSGSIWGGVVANSGAVQGNWGGADRGFYGSFGYQYLTGTNVASNTRFDGNAGAYWKI
                     MSRSYGSLTIGANVFGMGYSKNLRYFTYGQGGYFSPQEYLLLNAPVRWVGRYKRNFEY
                     SITASLGSQQFREDASPFFPTDPALQGKSGPYYPGQSVSGANYNVEFRGSYHLPGNWY
                     LSAYMNMNNALDYNQQIFGFSLRYLFRDSLASPEMMVPTIPDWKGVQPFNLP"
BASE COUNT         1045 a         1515 c         1374 g         1053 t
ORIGIN      
        1 ctgacgcctt gctccaactt gagcttgccc accagaggct cgggcacaaa gaagcggact
       61 tccagattct cggggggaag gacttgcacc accgggttgc cggccgcaac ccattctcct
      121 tcgcggtaga gtgtgtcaaa taccagcccg tcgcgcggcg aaacgatctc cttttgctgc
      181 agcttccacg tggcctgctc cagcgcggca cgatcggccg ccacttgttc cgcctgggcc
      241 ttcagctgct catctcttgc cggaagcgct gccaccacga gttcgctttc aacttgccgc
      301 accagtgctg tgttggtttc gacggcggcc cgggccgcga tcaaatcggt taaggaaacg
      361 ccccctgccg catactgtga ttcgtaactt ttgaggatat cgatggactt tttcttctca
      421 gccagcgtct gcatcaattg cgcccgcgtc acgtcgattt ccggcgagcg tttgccagtc
      481 tgcagatcgg cgaggcgggc ctgtgatatc cgcaccatca gctccgcctg tcgcgccgcc
      541 gctgcctagg gttcccggtc aagactgaac agaggatggc tgaccgccac cgtctctccg
      601 cggctgaccg acagatgcgt cagccttccg ccttgcggcg acgcaacgta aacaaacttg
      661 ccttcgacat acccttcgta gctattgctc ggcttggaag aacacccggg catgattaag
      721 gccaagccaa cggcaaggcc taacaacggg aaacagggtt ttatttgcag tctgcgcatt
      781 atcttctccg cagtcatcgt ttttttcgag atttcttcgg gcttggcgtc ggtgaatgtc
      841 gaagcccatc caatatcagc ccagtgatat gtcggccaac cgctttgcga ttcattggcc
      901 tgcggtgaaa tatctccgcc cagatcttga ttgtcgccat gtgtagcatc accagtccga
      961 gcatggagaa caccaccagc aggggattga ggtccgggtt ccatgccttg ctggcttgtc
     1021 cctcgcggat tgcctcagcg acgatgcgaa ctttgtcgaa aggtatgcgg cgcagcgcgc
     1081 gtccgcgcag cagtccacct tcgttcagga tttcgcgcat ccatgtcgag ggaatccacg
     1141 gcgcctgctc gatactcccc aatagtcgcg tcaccacgcc tgcaattagc tccgtcggat
     1201 cgtcgccagg cgtcactggc ccccaaacgt agtcaataaa agggcacagc cgctcttcca
     1261 ccaccgcgtc gagcagttgc tcgcggtcgg cgaagtagta atggaccatg gcagacgtca
     1321 atcctgctcg cctggcaatg atggagaagc tggttgctgc cacgccgtgc tcggcaaaca
     1381 actcggtggc cgccgtgatc agcaattcac gcgcgtcgcg atcggaactc gcagctgggc
     1441 gacccggttt gtccgacttc tgcattcggc gcgccatagc cataattaat taacgtaata
     1501 attaaataac gtcgtggacg gcctgtcaac cctaaagtgt gcagggacag atgaatcgct
     1561 tccggccacc aattccgctg gcttggagcc gccgcaccgt ctccgatccg atagtttcct
     1621 gggcccttaa cgatcgtctc cgccagttcg tcctgcgtcc aatcccgacg catgagtaaa
     1681 cccgcgctgg gctcttcgct ctcttgttgg taccttcgaa ccccgctacc gtgaagctat
     1741 gggcaagccg tgaacccgaa atgctaaaca accctgaaaa accaaaaacg ctcagcggtt
     1801 caacagaacg gcgaaaaagc gtgcgttacc caatcgccgg cgctgtctcg atgcactgga
     1861 aaacagcgga aggatcgtgg cgcgaggcag gaggaatgac ccgcaatatc gggaaaagtg
     1921 gtgtgttcgt cgaatgtgag ggcgctcctc ctgttggatc gcgtctcaag cttgtcatca
     1981 cgctcccgac ccagcttcga gaatatggtt ccgtttgcct gtgtggagtc ggcgaagtac
     2041 gtcatcggcg gcgggacacc gcgcagacaa gtgggttcgg ggcccgtgcg cagttccagt
     2101 tggatttgcc catgtccgcc ggaagaccgc agtagcagaa gtagcaggcg ggcgtttgca
     2161 atcgtcggcc tggagtcaga tgtgaaacaa ataccgggct tgcgtcaggt cgcttactga
     2221 aggctcggac caacaacgta gtcgaggaga aatggtatga agtcagcaaa aatggtatcg
     2281 gtcctattcc tgctattggc ggttgcattg tcgcttccag ccgttgcggg aactgtcaac
     2341 atgcaattca acggcactcc gaccggcaac aactactccg gagttcccag ctatccctat
     2401 gatctcagtg tcaacggcgc ccccaaccaa tggatgatgt gtatgggcta ctacgaacac
     2461 atcagcggcg gcgagacctg gcaggctact gcggtcagcg tgggcagcct cgatcccacc
     2521 agccatcttc cgaactacga agctgccttc ctgttcgaga aggctgtggc cgatcacggc
     2581 gccaatagtg atgtcaacgc tgccgtatgg tggatcttcg aaggtgctcc aagcctggat
     2641 ccaggtgctc aggccctggt gaccctcgcc cagagccaga cttacacgca gggcgaattt
     2701 tccaacgtca tgctgtacac cgccatcccc gggactgaga gcggcaatct cggtaccgct
     2761 caggacttca tgagcagcac tccggaacca ggaacgctcg cgcttcttgg atctggaatg
     2821 atcggcgccg ccggtttcct tcgccgcaag ctcttgccct gatgctgcca gtctgcacaa
     2881 cttgggctgc tgcatgccag cagcccattt ccattgcgcc cgcagtttca caggacttgg
     2941 ccccaacccg atgaccctga tacccgtgtc ctgccgagtg cagcccccat cttctccata
     3001 ccatcttcgc agggactaaa gtatgtaggg caagcgacga tataaagcgt gagacgagct
     3061 tctgtgagtt gaaggagctc tctatcggag gaaactcgat ggcatcctct cggccgaaga
     3121 caattcgtat cgcagtggca gactccaacc aaatgggttg ccgtttgtta tccgaggcgc
     3181 ttgacaaatt accagggctc gccgtggtcg cgtcctcgtc taacggtgaa tctctgctgc
     3241 aagcggtgca agccttcaaa cctgacatcg cagtaatcag tgccaccctc aaggacggac
     3301 cattcagccg tagggcctct atccctgaca tccgcagccg gacgccccaa tgtccctgga
     3361 tattaatgct tgatcaaagt gaaccgcagc tggtggtcgc ggcctttcgc gccggcgcga
     3421 agggagtgtt ctcccgcgct cagacggata tcagcctgct ggccaagtgc atccgtaagg
     3481 tgatggaagg cgaaatctgg gtagacaaca aacagatcct ctgtctactg gaggcagtca
     3541 ccggcagcga ccaacaatcg cacgatcaat cgtcctcacc cgtcaagctc accccgcgcg
     3601 agcagtcggt ggttcggctg gtggtgcagg gcatggtgaa ccgcgaaatt gccgacgcac
     3661 ttcatctcag cgaacacacc atcaaaaact atctgtttcg catcttcgac aaactaggcg
     3721 tgtcgaaccg tgtcgaactt gccctgtatg ctgtcgccaa gctcgatctc gccgacagcc
     3781 ccgcggcatg aatttctttc tcagaagacc agcgaatccg atcttggttc tagtttcatg
     3841 atttcgattt gaaagagcat ggctccggca gcctcgcagt aattcgggaa agggcccggg
     3901 tgagatccct taggacttcg gtccacacgc accttgaatc cacatgaatc ctggtgtgct
     3961 tgacctgccg gaccgcacac catcaatcat gtacgccgcc tacggcagat taaaaggttg
     4021 tactcccttc cagtccggaa tggttggtac catcatttct ggcgatgcca gtgagtcccg
     4081 gaacagatac cgcaagctga atccgaaaat ctgctgattg tagtccagag cgttattcat
     4141 gttcatatat gcgctcagat accagtttcc cggcaggtga tagctgccgc gaaactcgac
     4201 attgtagttg gcgccgctca cggattgccc gggatagtac ggtcccgact ttccctgcaa
     4261 cgcaggatcg gtagggaaga acgggctggc gtcttcgcgg aactgctgcg agcccaggct
     4321 ggcggtgatg gaatactcga agttgcgctt gtagcggccc acccagcgaa ctggcgcatt
     4381 gagcaacagg tattcctgtg gactgaaata gccgccctgg ccgtaggtga aatagcgcag
     4441 atttttgctg taacccatgc cgaagacatt ggccccgatg gtgagcgaac catagctgcg
     4501 cgacatgatc ttccagtacg ccccagcgtt gccgtcaaag cgggtgttgc tggccacatt
     4561 ggttccggtg aggtactgat agccgaagct gccgtagaat ccgcggtcgg cgcctcccca
     4621 gtttccctgc accgctccgg agtttgccac cactccaccc cagatgctgc ctgaaccggg
     4681 atctctcgat cccgcgtagg acagaagcgt gtccttcacg ttgtcgcgcg tggcccacaa
     4741 cgtgaccgga cctccggcaa tccgccagcg aacgccgccg acaaagttgg tcaccaggaa
     4801 tcccgatgga ctgatgccga accgtaatcc gaaatcctgg gtggacaatt gcacttcgcc
     4861 gcccaggccg ccggccgact gcgacccgaa tcttgtgcca accagcgagg ttccgaatcg
     4921 atagagcgac gtgccgtcgg gggtgccggc gttgagaaac actggccgcg cgatggccgt
     4981 caagcgc
//
LOCUS       DASRKK010000792         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_281739, whole genome shotgun sequence.
ACCESSION   DASRKK010000792 DASRKK010000000
VERSION     DASRKK010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281739"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFC15_19020"
     CDS             complement(<1..372)
                     /locus_tag="VFC15_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12306.1"
                     /translation="MSTTEKEPGLSTPSSTARVLTVLPDFPFPATTGLHLRHVSNLEL
                     VHRLGCYSALLYFSTEGREPAPVDTTPLARICDEVRHGGRRFPHANFSTASLVLHKAD
                     FLVRGALGLPGKRYPFSMSYDR"
     gene            complement(377..1117)
                     /locus_tag="VFC15_19025"
     CDS             complement(377..1117)
                     /locus_tag="VFC15_19025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZM12307.1"
                     /translation="MLHFRNGRTLVQRMRAAQPCDEVVLWDGTRISHPPQRAGLLEAV
                     QEIWLERIYTDGFYRPADGEVIVDAGANIGLFAIYVARQNPRCTVVALEPFAENFQYL
                     QANVAQARMKNVTCCEVALGAGFGKGQMQAVGTRSLDHVLRVDSHQADGVPVIPLSGL
                     FDLVRAQELDFLKVDIEGSEHDVFAAASPELLDRFKRIAMEYHDQIVPGTLALLRSVL
                     GATHEITVRPSHMEGCGILLARRRDLKK"
     gene            complement(1300..1782)
                     /locus_tag="VFC15_19030"
     CDS             complement(1300..1782)
                     /locus_tag="VFC15_19030"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM12308.1"
                     /translation="MYLPHLAMTLSNFGLFDAFQSRMDDARQHYQEALKIDRQLAEQN
                     SAVYLPDMAMTLNNLGRVDRLQNRMDEAHQHYEEALKIRRQLAQQNPDAYLPYLAMAL
                     NDLALVEDNQNRIEESRAHYKEALSLFRKLSQGDKRYVGNVARVEASLQQLNKRDHSQ
                     "
     gene            complement(2068..2322)
                     /locus_tag="VFC15_19035"
     CDS             complement(2068..2322)
                     /locus_tag="VFC15_19035"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZM12309.1"
                     /translation="MKKIPAKNPRLRLDSESYRELHHQVLERDGWRCQVCGSMQNLQV
                     HHLKFRSQSGGDEEQNLITLCAECHEQMHRKANCCKARLP"
     gene            complement(2319..2885)
                     /locus_tag="VFC15_19040"
     CDS             complement(2319..2885)
                     /locus_tag="VFC15_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12310.1"
                     /translation="MRAGQYWRLENLKSFDEFLERRFPESRRKAYYLMSIHEQLPPQV
                     RRELKQVGWTKGLELAKLARQQGQRFESATWLHKARQMPKAEFQREVEKELTGKDSEP
                     SELIYFKVYRSQMPVIERAIEAAALMLGTDKSRGYCLEMICADFLAGASIDGGNPDIL
                     LQSISRYYKFLPSPQQEAFRSEVVGQAP"
     gene            complement(3028..>3570)
                     /locus_tag="VFC15_19045"
     CDS             complement(3028..>3570)
                     /locus_tag="VFC15_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZM12311.1"
                     /translation="QLLERFVQDGRVQIQQPHSHQQIRFTRTLSSGNSFVAFVDAVGE
                     LESTPCVLEWKTSSARYPDEPDGIAALDPQLVCYSWMTGINEVAQVVFVRKRTVEVQY
                     LRATITDQQRQEFATLVDDTVRRIESGLFLPQSGIRFPQNPCTTCPFLGLCLDQRDLV
                     EAGLIRKPGVDLGLFDELGY"
BASE COUNT          723 a         1034 c         1036 g          777 t
ORIGIN      
        1 gcggtcgtac gacatggaga acggataacg ctttccggga aggccgagcg cgccccgcac
       61 caggaagtcg gccttgtgaa gaaccaagga ggcggtcgag aagttggcat gggggaatct
      121 gcggccgccg tgacggactt catcgcagat tcgcgccagc ggagtggtgt cgaccggcgc
      181 aggttcgcgc ccctcggtcg agaaatacag cagcgcgcta tagcagccga gccggtggac
      241 gagttccagg ttgcttacgt gcctgaggtg tagcccggtc gtggcgggga acggaaaatc
      301 cgggaggacg gtgagaaccc gcgctgtgct actcggtgtt gacaggccgg gctctttttc
      361 cgtggtagac attgcgctat ttcttgaggt cgcggcggcg ggccagcaag attccgcagc
      421 cctccatgtg cgatggacga acggtaatct cgtgagttgc gcctagaacg ctgcgcaaaa
      481 gggccagcgt accaggcacg atttgatcgt ggtactccat cgcgatgcgc ttgaagcggt
      541 cgagcagctc cggcgaagcg gcagcaaaaa cgtcgtgctc cgagccctcg atatcaacct
      601 tgaggaaatc gagctcctgg gcacgaacaa gatcgaacaa cccggacaaa ggtatgaccg
      661 gaacaccgtc tgcttggtgc gaatccacgc gaagcacgtg atcgagcgaa cgagttccta
      721 ccgcctgcat ctgtcccttg ccgaacccgg cgcccagagc cacttcgcag caagttacgt
      781 tcttcatgcg ggcttgagcg acgttggctt gcagatattg gaagttctcc gcaaaaggtt
      841 cgagagcgac cactgtgcat ctgggattct ggcgcgcaac gtaaatcgca aacaagccaa
      901 tattggcgcc ggcatcgacg atgacttcgc cgtccgcggg ccggtagaac ccatccgtat
      961 aaatccgctc gagccagatt tcctgaacgg cttccaataa accggcacgc tgtggaggat
     1021 gggagatgcg cgtgccgtcc caaagcacaa cttcatcgca cggctgcgct gcgcgcatcc
     1081 gctgcaccaa ggttcggcca ttgcgaaaat gcagcatcag cgaacccatc tggagaacac
     1141 ttttcaaata cataagttag acacagaaag aagatacgtc ggggcgcgcc ttgaaggaac
     1201 ccatcaattg ctgatagacc gatatcatct tatcaaccac aggccgcggt gttgggtact
     1261 tgttttgaag acagtttttc ttggctcatt ttgtttgcct tactgggagt ggtccctctt
     1321 atttagttgt tggaggctcg cttccactcg agccacgttg ccaacatatc tcttatcacc
     1381 ttgggagagc ttccggaaga ggctcagggc ctctttgtag tgggcgcggg attcttcgat
     1441 ccgattctgg ttgtcctcca cgagcgccaa atcgttaaga gccatcgcca agtacggcag
     1501 gtacgcgtcc ggattctgct gcgccagttg tcggcgaatc ttcaatgcct cctcatagtg
     1561 ctggtgcgct tcatccatcc ggttctggag tcgatccact ctccccaaat tgttcagcgt
     1621 catggccatg tccggcaggt acacagcgga attctgctcg gccagttgtc ggtcgatctt
     1681 caatgcctcc tgatagtgct gccgcgcgtc gtccatacgg ctctggaagg cgtcgaaaag
     1741 cccaaagtta ctcagtgtca tcgccaggtg gggcaggtac acggcgggac tctgttccgc
     1801 cagttgtcgc ggccgcgacc aggacgcaaa gaacagtaat cagtttcttg ccggtgagga
     1861 cggacccggt tctcagaggt gttttggtgt tcagcttcct gcggctttca gtgcgtgctt
     1921 tcgcgagaat cttggtgcgg ctcatttcgt ctagccgatt gtacacagcc gacgaagcag
     1981 caacgggacc gtgtttcttc cgcgcccttg gggcctgcag cactcattag tctacgcgaa
     2041 agatgcgcgc cctcccagag agatgagtca cggcagtcgg gccttgcagc agttggcttt
     2101 tctatgcatc tgttcgtggc attcagcgca cagagtgatc aggttctgtt cctcgtcgcc
     2161 acctgactgg ctgcgaaatt taaggtggtg cacctgcaga ttctgcatac taccgcacac
     2221 ctggcagcgc catccgtcgc gctccaacac ctggtggtgc aactctcgat agctctccga
     2281 atcgagtctt agtcgcgggt tcttggctgg gatcttcttc atggggcctg cccaacgacc
     2341 tcagatcgaa atgcctcctg ttgaggactg ggcaggaact tgtagtagcg ggagatggat
     2401 tgcaagagaa tgtccggatt accgccatcg atcgaggcgc cggcgagaaa gtctgcacag
     2461 atcatctcca ggcagtagcc ccgggacttg tccgtcccta gcatcaaggc cgcagcctcg
     2521 attgcccgct cgataaccgg catctggctc ctatagacct tgaaatagat cagctccgag
     2581 ggctcgctgt ccttgccggt cagctccttc tccacctccc gctggaactc cgccttgggc
     2641 atctgccggg ctttgtgcag ccaggttgca ctttcgaagc gctgcccctg ctgccgcgcc
     2701 agcttggcca actccagacc cttcgtccat cccacctgtt tcagttctct tctcacttgg
     2761 ggtggaagct gttcgtgaat cgacatcagg taatacgctt tccttctgga ctcggggaac
     2821 cggcgctcca ggaactcatc gaacgacttc aggttctcca gccgccagta ctggccggcg
     2881 cgcacttcgc aaagttactt gcccagttcc acgaacctgg tatcgcgctc gttctcatgc
     2941 cgtgcctcct agtccaggat cgcgtcgatc ttgctcagga ctgtcatggc ccggcggcga
     3001 ttcagcttgg gcggcatggg cggataccta gtagccaagt tcgtcaaaca gaccaagatc
     3061 gactcccggt ttgcgaatca gcccagcctc aaccaggtcg cgctgatcga gacagagccc
     3121 gagaaaagga caggtagtac acggattctg ggggaagcgg atgccgcttt ggggcaggaa
     3181 cagtccagac tcaatccgac ggaccgtgtc gtcgaccagg gtcgcgaact cttgccgctg
     3241 ttggtcggta atggtggccc gcaggtattg gacttctacc gtgcgcttac ggacaaagac
     3301 gacctgggcg acctcattga ttcccgtcat ccaggaatag caaaccagct gcggatccag
     3361 ggctgcaata ccgtcaggct cgtcgggata gcgggcagag ctggtcttcc actccagcac
     3421 acaaggtgta ctctccagtt cgccgacggc atcgacgaag gcgacaaagc tgttgccgga
     3481 actcaacgta cgtgtgaatc ggatttgttg atggctgtgg ggctgctgaa tctggacgcg
     3541 gccatcttgg acgaagcgct cgagcagctg
//
LOCUS       DASRKK010000793         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_185010, whole genome shotgun sequence.
ACCESSION   DASRKK010000793 DASRKK010000000
VERSION     DASRKK010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185010"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1514
                     /locus_tag="VFC15_19050"
     CDS             138..1514
                     /locus_tag="VFC15_19050"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZM12312.1"
                     /translation="MTSPASPSIVDTTELWLMFRTVASESFFAELQPASGPGSRSGIF
                     SIAMVIWLMLYQRLHSKGTLVAALQVMTRLGQSGHLRLAHPCKRIREGNISANTGGYC
                     QARQKLPMPVVKTVSNHIFDQLRQQLRDPSHSGPPVYVIDGTTLRLRHERDLVEAFPP
                     GHNQHGGNHWPVLLMVAFHDVYTGLAAQPSWGAMYGPKAVSEQSLAEQALDRLPADAI
                     VLADGNFGIFAFVHAVQKSQRPILARLTASRARKILGNTTPSPGKRRKVTWLPSIYER
                     RAHPDLPPEASVEGWVVVSQNPARPTEMLYLFTTLDWTPARILELYKLRWNIETDLRS
                     LKRTIGLHQINGRAKAMVEKELLLAVSAYNLVRAVIAIAACREGLPARQFSFSVSQDA
                     VMAAWSDLQQATTTDDYNREMELLLFFVSKAKLPKRSGSRSYPREIWGRGGHFPFRRS
                     IPATESGQ"
     gene            1511..1735
                     /locus_tag="VFC15_19055"
     CDS             1511..1735
                     /locus_tag="VFC15_19055"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HZM12313.1"
                     /translation="MTSVPVELPSTSLFKAAYDDQRAILQIQFRDGTTYDYASVPPEL
                     FHALLAAPSKGVFFNHHIRGRFPFVKGDAQ"
     gene            complement(1716..>2056)
                     /locus_tag="VFC15_19060"
     CDS             complement(1716..>2056)
                     /locus_tag="VFC15_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12314.1"
                     /translation="FKQDEAPLADVQGLLETLSEAWREVAEIDHPMAAAAASSSSAGS
                     FPWSGATETASAPAESFTYAPSGSSAYTPVETTAYSPAPADSFTYTPSGSPAYEPVEA
                     QCHLVIEHRP"
BASE COUNT          422 a          601 c          588 g          445 t
ORIGIN      
        1 cgtcccaacc gctcggcgca tcaagcgctg gaggagatcc gcactcatct caaagcgggc
       61 tatcaggcca atgtcactta gttttgggcc gaggtctcag agcaaataga acaaaataga
      121 gaggttatgt tttatacatg acctctcccg cttcccccag cattgtggat acgactgagc
      181 tttggctgat gttccggacg gtcgcctcgg agtccttttt cgctgaactt caacccgcgt
      241 ctggccctgg atctcgcagc ggcatcttca gtatcgccat ggtaatttgg ctgatgctgt
      301 accagcggct gcactctaaa gggacgctgg tcgcggcgct gcaagtgatg acgcgattgg
      361 ggcaaagcgg gcacttacgc ttggcccatc cctgcaagcg aattcgcgaa ggcaatattt
      421 cagccaacac gggcggatat tgccaggcaa ggcagaagct gcccatgccg gtagtcaaga
      481 ccgtgtcgaa ccacatcttc gatcaacttc gccagcagtt gcgcgaccca tcgcactccg
      541 gcccacccgt atatgtgatc gatggcacga cattgcggct tcgccacgaa agagatttag
      601 tcgaggcctt tccgcccggg cacaaccagc atggcggtaa tcactggccg gtcctgctga
      661 tggtggcctt tcacgacgtg tataccggtc tggccgctca gcccagttgg ggtgcgatgt
      721 atggtccaaa ggcggtcagt gagcagagtc tggcggaaca ggcgttggac cgcttgccgg
      781 cggatgcgat tgtattggcc gacggcaact tcggaatctt tgccttcgtt catgccgtgc
      841 aaaagagtca gcgcccgata ctcgcgcgac tcacggcatc ccgtgcgcgg aagatattgg
      901 gcaacacaac gccatctccg ggaaagcgtc gaaaagtaac ttggctgccc agtatctatg
      961 agcgcagggc tcatcccgat ttgccgccgg aagcgtcggt ggagggctgg gtggtggtca
     1021 gccagaaccc cgctcggccg acggagatgc tctacctgtt tacgaccttg gactggacgc
     1081 cggcgcggat cctggagcta tataagctgc gctggaacat cgagaccgat ttgcggagtc
     1141 tgaagcggac catcggtctg catcagatca acggcagggc caaggcaatg gtggaaaagg
     1201 aactgctgtt ggccgtctcg gcctacaatc tggttcgtgc cgtcatcgct atcgccgcct
     1261 gccgggaagg cttgccagca cggcagttca gcttttccgt ctcccaagac gcagtcatgg
     1321 cggcttggtc ggacttacag caagctacta ccacggacga ttacaaccgc gaaatggaac
     1381 tgctgctctt ctttgtgtcc aaggccaaac tgccgaagcg ctccgggagt cgatcttatc
     1441 cgcgcgaaat ctggggccga gggggacact ttcctttccg ccgatccatc cctgcaacgg
     1501 agtccggcca atgacttctg ttccggttga gttgccatct acttctttgt tcaaagccgc
     1561 ctatgacgat caacgagcaa tcctgcaaat tcaattccgt gatggcacca cctatgacta
     1621 cgccagcgtg ccgccggaac tctttcacgc gttactcgcc gctccttcaa aaggcgtctt
     1681 cttcaaccat cacattcgcg gtcgcttccc ctttgttaag ggcgatgctc aataactaag
     1741 tgacattggg cttcaaccgg ctcgtaagcg ggacttccgg agggcgtata ggtgaagcta
     1801 tccgccggcg ccgggctata agccgtagtt tcaaccggcg tgtaagcaga gcttccggag
     1861 ggcgcataag tgaagctctc cgcgggcgcc gatgcggttt cagttgcgcc gctccacggg
     1921 aaagaacccg cggaagaaga acttgccgcg gctgctgcca ttggatggtc gatttccgcc
     1981 acctctctcc atgcctcgga gagtgtttcg aggaggcctt ggacgtcggc taacggcgcc
     2041 tcatcctgct taaaat
//
LOCUS       DASRKK010000794         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1163002, whole genome shotgun sequence.
ACCESSION   DASRKK010000794 DASRKK010000000
VERSION     DASRKK010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163002"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFC15_19065"
     CDS             <1..295
                     /locus_tag="VFC15_19065"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HZM12315.1"
                     /translation="KIRELKLRKRTQIRLADIAREINPILQGWLNYYGRYNPAAMYPM
                     WRYVNATLVAWAMRKYKRYAGRKTQAGRMIAAIAIKRPRLFVHWHQSKAGAFA"
     gene            846..2147
                     /gene="ltrA"
                     /locus_tag="VFC15_19070"
     CDS             846..2147
                     /gene="ltrA"
                     /locus_tag="VFC15_19070"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM12316.1"
                     /translation="MTKASNDLQDLRRRIYVKAKAETTWRFWGLYVHVCKMETLRAAY
                     ALAEKNDGAPGVDGVTFAAIETQGVDAFLEQIADELNRRTYVPLPARRQEIPKDGGKV
                     RVLSIPAIRDRVVQGALKLILEPIFEADFQPGSFGYRPKRTAHEAVDRVARAIVQEKT
                     RIIDIDLRSYFDNVRHDRLLAKVARRVDDADVLHLLKVMLKANGRRGVPQGGVISPLL
                     SNLYLNEVDKMLERAKETTRYGKYTYIEYARFADDLVILIDAHPRHDWLMAAVEKRLR
                     EELAKLQVEINEEKSRNVDLGRGESFGFLGFDFRRVRSRRGVWRANYTPKLKKRTALL
                     RKLKDVFRRHQSQSVSRVVQLINPVLRGWVNYFAVGHSSECFSFIKDWVEKKVRRHMG
                     RAQNRKGFGWKRWSRQWLYESLRLFNGYRVRRPMSKVAPAG"
     gene            2397..2603
                     /locus_tag="VFC15_19075"
                     /pseudo
     CDS             2397..2603
                     /locus_tag="VFC15_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          651 a          705 c          877 g          484 t
ORIGIN      
        1 aaagatcagg gagctaaaac ttcgcaagcg cacgcaaata cgactggcgg atatagcccg
       61 agagatcaac ccaatcctac aaggatggtt gaactactac ggacgctata atccggcggc
      121 gatgtatccg atgtggaggt atgttaacgc cacattggtt gcgtgggcca tgcgaaagta
      181 caagcgctat gcaggacgta aaacacaagc cggccgaatg attgcggcca tagcaatcaa
      241 gcggccgaga ctgttcgtac actggcatca aagcaaagcc ggcgcgtttg cctgatggga
      301 gctggatgag gcgagagtct cacgtccagt tgtgtgtcca gtgaaggctg gtgtgttcag
      361 caggaggcaa acctgccggg gcaagagtca ggcgccccgt agcttggatg gcagggtgga
      421 gggaaaccaa gactctgaag cccatcgaca aggtctccct cggggagatc acgagtcacc
      481 gggccgtaat gaaagtgaac gcgaaagtgg cctcgaaagt gagtgaatcc gagggccgag
      541 cccgcaactg aagggcgaag gcagcatggg gtgccgaaat ctgaccgacg cggcatcttg
      601 atctcggcgg ggtggaagcg acagcacggt gacaaggaca tgccaagcaa ctggagaagc
      661 cctcctcgtc ccggcgagaa atcgccggag caaggtagat cgtataaccg gcgcaaccgg
      721 gaaatcgatc gatggcgaga gggtggcgga tgggtccgca gtagcgatga agcggagtaa
      781 tgtccgtgga acgaaggggc cctgctgttt gtaatttctc tggcaatcag ggaggcaagg
      841 acgagatgac aaaggcgtcc aacgatttgc aggacctgag acggagaata tacgtcaagg
      901 cgaaggctga gacgacctgg cgtttctggg gtttgtacgt ccatgtctgc aagatggaaa
      961 cgctacgcgc tgcgtatgcg ctggccgaaa agaacgatgg ggctccggga gtagatgggg
     1021 tgaccttcgc ggccatcgaa acgcaaggcg tagatgcttt cctcgaacag attgcggacg
     1081 aactcaatcg gcgcacatat gtaccactcc cggcccgaag gcaggaaata ccgaaggacg
     1141 ggggcaaagt ccgcgtgctt tcgattcctg caattcggga tcgcgtggtg caaggtgcgc
     1201 tcaagctcat actggagcca atcttcgagg cggacttcca accgggatcg tttggatatc
     1261 gtcccaagag gacagctcat gaagcggtcg accgagtggc ccgagcaatt gttcaggaga
     1321 aaacacgtat catagacatc gatctgcgta gctactttga caatgtccga catgatcggc
     1381 tcttggccaa ggtggcacgg cgggtggatg atgcggacgt gttgcatctg ctgaaggtga
     1441 tgctcaaagc caacggtcga cgcggcgttc cgcaaggcgg ggtcatctct ccattgctca
     1501 gcaatctcta cctcaacgag gtggataaaa tgctggagcg ggcgaaagag accacgcgct
     1561 acggcaagta cacctacatc gagtacgcac gttttgccga tgatctggtg atcttgatcg
     1621 atgcccaccc gcgacatgac tggctgatgg cggcggtgga gaaacgcctc cgggaggagt
     1681 tggccaaact gcaagtcgag atcaatgaag agaagagtcg aaacgtagac ctcggccgag
     1741 gcgagagctt cggcttcctg ggtttcgatt tccgccgtgt gcgcagtcgt cggggtgttt
     1801 ggcgtgccaa ctatacgccc aagctcaaga aacgaactgc gctgctgcgg aagctcaaag
     1861 acgtgttccg ccgccaccaa tcgcaatcag tgagccgggt tgtgcaattg atcaacccgg
     1921 tgctgcgcgg ttgggtgaat tacttcgcgg tcgggcattc cagcgagtgc ttcagcttta
     1981 tcaaagactg ggtagaaaag aaggtccggc gccatatggg gcgggctcag aaccgaaagg
     2041 gcttcggctg gaagcggtgg agtaggcagt ggttgtacga atccttgcgg ctgttcaacg
     2101 gctaccgggt tcggcgaccg atgtcgaaag tcgccccggc aggataggtc ccataagcct
     2161 tgaggcgaag cagacaggag agcgcagtgc gaggaaatcc gcatgctgcg ttcgacgtgg
     2221 cgggggctgg aaacgtggca tggtagagat ggtgtgacac tcgcagacga aagggcgaga
     2281 caacaggcaa cacaaacttc gacctaaacc ggcgcgccag tcctcgaccc tactgatgag
     2341 cggggagggg aaacggagcg cgcttcaacg cgccaccgcg cccttcctcg actctactca
     2401 aacggcagaa gaacgacgcc gcggacgcgg aggccatctg cgaagccgtc accagggtca
     2461 acatgcggtt cgtcgcgacc aagacgcccg agcagcagag ctgcctgatg ctccatcgca
     2521 cgcgccatct gttcatccgc cagcaaactg cggtgatcaa tgtgatccgg gcccaccttg
     2581 ccgagttcgg gatcgtcgcg ccggtcggac gcaaaggtgt cgaggaactg ctcgatgtcg
     2641 tcgccgatcc aagtgacaag cgagttcctg cgatagcccg tgcgtgtctc gcagcacttg
     2701 ggactcaact gcgcagg
//
LOCUS       DASRKK010000795         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1065687, whole genome shotgun sequence.
ACCESSION   DASRKK010000795 DASRKK010000000
VERSION     DASRKK010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065687"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="rocF"
                     /locus_tag="VFC15_19080"
     CDS             <1..666
                     /gene="rocF"
                     /locus_tag="VFC15_19080"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZM12317.1"
                     /translation="VKTLEAGQVPVVLGGDHSIAAGTVSGVAEFYRRKNQRIGLLWID
                     AHTDINTPGSSPSGNVHGMPLGAIMGLFESPLTDLYGFSPKVQPQNCVLVGIRDVDNH
                     ERENIKKSGVHVFTMRDIDERGMRSVMEEALRIAGRGTVGYHVSLDLDWIDPEDAPGV
                     GTPVWGGATYREGHLAMEIIADHGRMLSFELVEVNPVLDERNQTAELAVELALSAFGK
                     KIL"
     gene            691..1512
                     /locus_tag="VFC15_19085"
     CDS             691..1512
                     /locus_tag="VFC15_19085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM12318.1"
                     /translation="MSVGVSSAAQFALPPLPELQSTTFFGRKIYYYDIGSGPTLVLVH
                     GLGGDADQWAYCFEALSATHRVVALDLLGFGRSDKPSISYRIAGFVEVLQGFLQAFGI
                     ERASLLGHSLGGWIVAAFALQFPDKVDRLVLNDAAGLDSGAVKPPVDLNISSRAHMRK
                     MLESMFYNQALVSDGLVDLAYSLHLERGDGSTIRSVLETFSKPDEKLDRSISGLNAPT
                     LILWGEQDALTPLTLARNFQRLIRGSRLEGIPECGHFPALEKPAEFTRRVLEFLR"
     gene            complement(1552..1965)
                     /locus_tag="VFC15_19090"
     CDS             complement(1552..1965)
                     /locus_tag="VFC15_19090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM12319.1"
                     /translation="MRRYVLDANALLIYLGDRQGAARVQALLNESLRNRSPLLMSAVN
                     WGEVVYSIWKQGGEAEARRVEKNIAHLPLAILPADRERATGAAELKARHGLGYADSFA
                     AQLALENAATLVTADPEFAKLGRRLAVLSLPRHKS"
     gene            complement(1962..2234)
                     /locus_tag="VFC15_19095"
     CDS             complement(1962..2234)
                     /locus_tag="VFC15_19095"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZM12320.1"
                     /translation="MKKPASPASTFTATISSKGQMVLPAPVRKRLGLKEGMHVSISFT
                     EDDGDRLIVQPLTPRLIQELRGSIGCAGAALDYLQEERKRDREQGR"
BASE COUNT          507 a          763 c          769 g          530 t
ORIGIN      
        1 gtgaaaacgt tggaggccgg acaggttccc gtggttctcg gtggcgacca ttccattgcc
       61 gctgggacag tgtctggcgt ggccgagttt tatcgccgca agaaccagcg aatcgggctg
      121 ttgtggattg acgcgcacac cgatatcaat acgcccggca gctcgccgag tggcaatgtg
      181 catggtatgc cgttgggcgc gatcatggga ctgttcgagt cgccactcac cgacctgtat
      241 ggcttcagtc ccaaggtgca gccgcaaaat tgcgttctgg tgggcatccg cgacgtcgat
      301 aaccacgagc gcgaaaacat taagaagtca ggcgtacacg tctttaccat gcgcgacatt
      361 gacgaacgcg gcatgcgctc ggtgatggaa gaggccctcc gcattgctgg acgtggcacc
      421 gtgggctatc acgtctcgct cgacctggac tggatcgatc ccgaagatgc tcccggtgtc
      481 ggcaccccgg tttggggcgg cgccacctat cgcgagggac acctggcgat ggagatcatc
      541 gccgaccacg ggcgcatgct cagcttcgaa ctggtggaag tgaatccggt gctcgatgag
      601 cgcaaccaga cggcggaact ggcggtcgaa ctggcgctgt cggcctttgg caagaagata
      661 ctctgagctg ggcatatcga ggtgcacggc atgagcgttg gtgtatcgtc ggctgcgcag
      721 ttcgctctgc ccccgctccc cgagttgcaa agcacgacct tcttcggacg aaaaatctac
      781 tactacgaca ttggcagcgg gccaaccctg gtgcttgtcc atggccttgg cggtgacgcc
      841 gatcagtggg cctactgctt tgaagcgctc tccgccacgc atcgcgtggt cgcattggat
      901 ttgctcgggt ttggccgctc cgacaagccc agcataagct accgcattgc cggattcgtc
      961 gaggtgctgc agggttttct acaggcgttc ggaatcgagc gcgcatcgct cctcggtcat
     1021 tcgctgggcg gatggatcgt ggccgcattc gcgttgcaat tcccggacaa ggtggacaga
     1081 ctcgtgctga acgatgccgc agggctggac tccggcgcgg tcaagcctcc cgtcgacctg
     1141 aacatctcca gccgcgcaca tatgcgcaag atgctggagt ccatgttcta caaccaggcc
     1201 ttggtcagcg acgggttagt tgacttggct tattcgctgc atctggagcg cggcgacgga
     1261 tccacgatcc gcagcgttct ggaaaccttc tccaagcctg atgaaaaact agaccgcagc
     1321 attagcgggc tgaacgctcc aactctgatc ctgtggggcg aacaggatgc gcttactccg
     1381 ctgacgctgg cgcgcaactt ccagcggctt atccgcggct ctcgcctcga aggcatcccg
     1441 gaatgcgggc attttcccgc gctcgaaaag ccagccgaat tcacgcgacg cgtactggag
     1501 ttcctgcgct agaaaaatca acgcaatccc gtgctctcat cgtctcagca actaggattt
     1561 gtgtcgcggc agtgacaaga cggccaaccg cctgcccagt ttggcaaact ccggatcggc
     1621 ggtgaccagg gtcgcagcgt tctccaaagc gagttgggcg gcaaaactgt cggcgtatcc
     1681 caaaccgtgg cgcgctttta gctcagccgc accggtcgcg cgctcgcgat cggccggtag
     1741 aatcgccaat gggagatgcg cgatgttctt ttcaactcgc cgcgcctctg cttcgccacc
     1801 ttgcttccaa atggagtaca cgacttcccc ccaattaacc gccgacatca ggagagggga
     1861 gcgattgcgt aacgactcgt tgagaagcgc ctgaacccgg gcggcaccct gccgatcacc
     1921 caggtaaatg agaagagcgt ttgcgtcgag cacataacgc ctcaccgccc ttgctcccgg
     1981 tcgcgttttc gctcctcttg caaatagtcg agcgcggcgc ctgcacatcc gatggagccc
     2041 ctcaactcct gaatgaggcg cggagtcagg ggctgcacaa taaggcggtc gccgtcatcc
     2101 tccgtgaaag aaatgctgac gtgcatgcct tccttcaaac ccagtcgctt tcgcacgggc
     2161 gcgggcagca ccatctgccc tttggaactg atcgtagccg tgaacgtgga ggcggggctc
     2221 gctggctttt tcatgactgt atcttactat aatgtaagat gacggttggc tcgaatggaa
     2281 ttcggtttgt ggcactggaa cgagggtgat ctgcggcttg ggcggttcat ctgcaagagg
     2341 tccttcagat cgccgcgtgg actcaacggc ccatcgcctc cggcaaaccg ccttctccga
     2401 gttaagatgc actcatgaag aaactccggg taggtgtcct gtttggcggg cgcagcggcg
     2461 agcacgaagt ctcactgctg tcggcggcct cggtcctgaa cgcaatcgat aagaagaaat
     2521 atgacgttgt tcccatcggc ataaccaaag caggcagatg ggtcactgc
//
LOCUS       DASRKK010000796         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_869972, whole genome shotgun sequence.
ACCESSION   DASRKK010000796 DASRKK010000000
VERSION     DASRKK010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869972"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..469)
                     /locus_tag="VFC15_19100"
     CDS             complement(116..469)
                     /locus_tag="VFC15_19100"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HZM12321.1"
                     /translation="MFSCSGAADIAEIGDRAVRSLHKAGDAKMFCLAGIAGKVELIEV
                     NTRGADRILVVDGCDSDCAKKTMEFGGFTGFIHLRVSDLGMEKGKTPVTDERIAVVAA
                     KLRELLGRQLEVAEA"
     gene            638..1936
                     /locus_tag="VFC15_19105"
     CDS             638..1936
                     /locus_tag="VFC15_19105"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZM12322.1"
                     /translation="MKKPYQIEAQRAVKQLEAMATDGNPAVQMVLPMAEMVGWLRKGV
                     GELMRQAGLQLMDLLMQEEVRQLAGERSQRQAERSASRWGSERGYCVVMGQKVPVRRP
                     RVRTTEDKEVRLGSYEVFHRGEPLTETVWEKLMLGLSTRKYGQAVRQFTEAYGLEKSA
                     VSEHFIEASRVKLKDMMERRLDKMRLCALLIDATPFEGQQMVAALGIGEDGRKTILGI
                     RQGATENATVVGELLGDLVERGLDFITPRLYVLDGGKALTAAVKRHAGESPAIQRCQV
                     HKRRNVLDHLTDEQKPGVAKRLNAAYALEDYAAAKQALNKLHRELMDLNPSAARSLGE
                     GMEETLTVHRLHVPMQLRKTLASTNVIESAFSTVETVCRNVKRWHSGDQRERWVGSGL
                     LVAQKQFRRIIGYKQIPALVRELEALAPSKKAVVKARKAS"
BASE COUNT          490 a          562 c          684 g          411 t
ORIGIN      
        1 gcggctgcga cgccgccggg ggatcggccc gagctttttc ggagtggaca tagatcgcgc
       61 cgagagccgc aaccgtcagg aagaaaaggg cgatgggaat gagctttcga gtcacttatg
      121 cctccgcgac ttccaattgc cgcccgagca gttcgcgcaa tttcgccgca accacggcaa
      181 tccgctcatc ggtgacgggc gtcttgcctt tctccattcc caaatcgctg acgcggaggt
      241 ggatgaaccc ggtgaaaccg ccgaattcca tcgttttctt ggcgcagtca ctatcgcagc
      301 cgtccaccac aaggatgcgg tcggctccgc gggtattgac ctcgatcaac tcgactttgc
      361 cggcgatccc cgccaggcag aacatctttg cgtcgcctgc cttatggaga ctgcgaacgg
      421 cacgatcgcc gatctcggcg atgtcggccg cgccagaaca agagaacatc aggtccgtag
      481 atcctgaaca agagcacttt ggtcgtatca ccatatgtct tcctttctgt ttcaaattgg
      541 ggaatgtccg gtagatcgta gaaagtagcg actcccccgc tcaaggcctt tgataggctg
      601 agagcatttt cttttgccga gaaaaaggag agtcgctatg aagaaaccat atcagattga
      661 agcgcagcga gcagtgaaac agctcgaggc aatggccacg gatggaaacc ctgcggtgca
      721 gatggtgctg ccgatggccg agatggtggg ctggttgcgt aaaggcgtgg gtgagttgat
      781 gcgtcaagcc ggcttgcagt tgatggattt attgatgcag gaagaagtcc ggcaattggc
      841 tggagagcgc agccagcggc aagccgaaag gtcggctagt cggtggggta gcgagcgtgg
      901 ctactgcgtg gtgatgggcc agaaggttcc cgtccgccgc ccgcgggtgc ggacgacaga
      961 agataaggag gtccgtttag gcagttacga agtatttcac cgcggcgaac cgctcaccga
     1021 aacggtttgg gagaagttga tgctcgggtt gagcacgagg aaatacggtc aagccgtgcg
     1081 ccagttcacc gaagcatacg ggttggaaaa gagcgcagtc agtgaacact tcatcgaggc
     1141 gagccgggtg aagttgaagg acatgatgga gcggcgactg gacaagatgc ggctgtgtgc
     1201 attgctaatc gacgcgacgc ctttcgaagg ccagcagatg gtggcggcct tgggcatcgg
     1261 ggaagacggg cgcaaaacga ttcttggcat acggcaagga gcgacagaga acgcgacggt
     1321 ggtcggcgag ttattgggcg acctggtgga acgcgggctc gatttcatca ccccacggct
     1381 ttacgttttg gatggcggta aggcgctgac tgctgcggtg aagaggcacg ccggcgagtc
     1441 gccagcgatc cagcgctgcc aggtccacaa gcgccggaat gtactggacc atctgaccga
     1501 cgaacagaaa ccaggcgtgg cgaaaaggct caatgcggcc tatgcgctgg aagactacgc
     1561 ggccgccaag caggcgctca acaagctcca tcgcgaattg atggatctca atccgagtgc
     1621 ggcgcggagt ttgggcgaag gcatggagga aaccttgacg gtgcaccgcc tccacgtccc
     1681 gatgcaactg cgaaagaccc tggcaagcac caacgtgatc gagtccgcat tctcgaccgt
     1741 ggagacggtt tgcagaaacg tgaaacgttg gcacagtggc gaccagcggg agcgttgggt
     1801 gggatcggga ctcctcgttg cccagaaaca attccgtcgc attataggct acaaacagat
     1861 ccccgcactc gtcagagaat tggaagcttt ggcaccatct aagaaagcag ttgtcaaagc
     1921 gaggaaggcg tcgtagagtg agtttacgcg ggcgtcgtta ctttcaacgg aattccgggc
     1981 attccctgcg ctggatttgc ctggaagaca gcgtaagcgc gcttggcctc gcgttggaca
     2041 tcaggcggta catcatgaaa cagacgtcag aaattccggg tagtccggga ctgcatcaga
     2101 gcagatcact cgagcggcag cgtctctccc ttagcgtctt cctcaag
//
LOCUS       DASRKK010000797         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_185925, whole genome shotgun sequence.
ACCESSION   DASRKK010000797 DASRKK010000000
VERSION     DASRKK010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185925"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..795
                     /locus_tag="VFC15_19110"
     CDS             409..795
                     /locus_tag="VFC15_19110"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZM12323.1"
                     /translation="MPFAFGWWTSLRIALAGMFLLTASAHWGKRRPDLIRMVPPFFPH
                     PDLLVTVTGILEVLGALGLMLPRFASYAALGLSVMLLSVFPANIHAARQHLSIAGSPV
                     PRLLPRTLIQIAFLAATVAVFVGGRH"
     gene            1139..3535
                     /locus_tag="VFC15_19115"
     CDS             1139..3535
                     /locus_tag="VFC15_19115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM12324.1"
                     /translation="MHDNGDTTGGFQNGADAAATRASFPGRSVPGNVIGPYALLELIG
                     EGGMGEVWLAEQKEPVRRRVAVKLIKPGMDTHEVVMRFESERQALALMDHPAIAKVFD
                     GGSTSDGRPYFVMEYVAGLPITAYCDKHKLTTQQRLELFVHVCEGVQHAHQKAIIHRD
                     LKPSNILVTEVDGKPMPRIIDFGVAKATSQRLTDHSMYTHIGAIIGTLEYMSPEQADS
                     AGQDIDTRTDVYSLGVVLYELLVGTLPLEFRKLAFDQALKVLREEDAPKPSTRLRTFG
                     DKGTKRAQARGAEPATLARQLQGDPDAIVMKAVEKERARRYATPLELSADIERYLNNE
                     PVLARSASAGYRAQKYVRRHRLGVAMAGVGLVLLIAFAVLQAIQLRRTTRERNRADRI
                     TGFMTGMFKVANPSEARGNTITAREILDRGSKDIDTGLSNDPELQAKMMYIMANTYYG
                     LGLYSRAQSLVERGIEIQRRVLGPEHPDTLRSMYRLACILRDGGHLAEAEKLSRETLE
                     IQRRVVGLDHPNTLESMANLAVVLTYEGHYAEAEKLDRQILETERRLLGPDHPNTLLS
                     MNNLAAELVDSGRDVEAEKVLHETLEIQRRVLGSDHPDTLRSMSNLANTLRDEGRDAE
                     AERLFHETLEIQQRVLGPEHRETLTSKLNLAVTLSHEGHYADAEKLFRETLDVQRRVL
                     GPDHQSALETLESLGISISHDGHRYAEAEQLFREAIERATKANQPRILAQAWYNFACG
                     AAVAGRHDEAIKYLNQAVDNGFEVTVESIASDPDLKSLRGNVTFEALLTKMRQGTAAK
                     "
BASE COUNT          813 a         1113 c         1141 g          781 t
ORIGIN      
        1 gctcgcagga tactgctttg tgcttctgcc gtaggcgttg cttgttccga cgggtccgca
       61 cagtaatctt gccggcttag cgatttcgta ctgaggcttg cacagactgt ggtttctgcg
      121 cgcgaggcct ttttcaatgc atccgcgaaa cgggcttgag gagatcagag cgcgagcagc
      181 attcagcagc agcgcacttc ctcgaagagg acttctggct cgctttcgcg cactcgctta
      241 tttgcgggtc tttggaggga ttgcattgct gaagctcgaa tccttttgcc ggtcggctaa
      301 atctgagagc taaaacgggt aataatattg tcgcaatacg ccgccatagt accgctcatt
      361 cttctgataa ttctatttgt attattgacc gtcgccggcc acctgcagat gccgtttgcg
      421 ttcgggtggt ggacatcgct gcgcattgcg cttgccggca tgttcttgct gacggcctca
      481 gcgcactggg gcaagcgtcg tcccgacctt attcgaatgg ttcctccgtt tttccctcac
      541 ccggaccttc tggtcaccgt gactggaatt ctcgaagttc tcggagcgct tggcctgatg
      601 ctgccgaggt tcgcatctta tgcagcgcta ggattgtcag tcatgctgct ttcggtattc
      661 ccggcgaaca ttcatgcggc gcggcagcac ctgagcattg cggggagccc agtaccaaga
      721 ttgctgcctc gaacgctcat acagattgcg ttccttgcgg caacagtagc cgtgtttgtt
      781 ggcggaagac attgaggaat ttccttggcc gagtgtcgct gggcccgagc accctgccca
      841 ctgtcggtcg cgattcgcaa gctattcgtt acttcgggat ggcctgtcat ccgctcagag
      901 tctgctgatt ggccacatct caagccgcga tgggcttttg tccaaggcgc gtatcaatgc
      961 gttggtaacc ggtccccggc gatccgtgca gacaagactc tgccccgatc aattgcccat
     1021 cacagaaatt ctcccagcca ccactgacag cggggacgac gatcggcttc acaaccggga
     1081 cggttgcagg gtcacgaggc ggtgcgtaca ctcttgccca gcagccggtg ggctccacat
     1141 gcacgataac ggcgatacaa cgggaggctt tcagaacggc gcagacgcgg cggcaacccg
     1201 cgcgagtttt cccggtaggt cggtccccgg caacgtcata ggcccttacg ctctgcttga
     1261 gttgatcggc gaaggcggca tgggagaggt gtggctggcc gagcagaagg agccggtgcg
     1321 ccgccgcgtc gccgtcaagc tgatcaagcc tggcatggat acgcacgagg tggtgatgcg
     1381 ctttgagtcc gagcgccaag ccttggcgct catggaccat ccggcgatcg ccaaggtctt
     1441 cgacggcggc tcgacatccg acggtcgtcc gtacttcgtg atggagtacg tcgccgggct
     1501 gcccataacg gcgtactgcg acaagcataa gctcaccacg caacagcggc tggaactttt
     1561 tgttcatgtc tgcgaagggg tgcagcacgc gcaccaaaag gcgatcattc atcgcgacct
     1621 gaagccctcg aacatcctgg tgaccgaagt agacggcaag cctatgccgc ggatcattga
     1681 ctttggcgtg gcgaaagcga cctcgcaacg gctcacagac cacagtatgt acacccacat
     1741 cggcgcgatt atcgggacgc tggagtacat gagccccgaa caggccgact ctgctgggca
     1801 ggacatcgac acccgcaccg atgtctattc gctcggcgtg gtgctctacg aattgctcgt
     1861 tggtacgctg ccgctcgagt ttcgcaaact ggccttcgat caggctctga aggtgctccg
     1921 ggaagaagat gctccgaagc cgagcacaag gctccgcact ttcggcgaca agggcaccaa
     1981 aagggcacag gctcgcggtg ccgagccagc gaccctcgcc cgccaactgc aaggcgatcc
     2041 tgacgcgatc gtcatgaaag cggtcgaaaa agaacgcgcc cgccgttacg ccacaccgct
     2101 cgaactctct gccgacatag aacgctacct gaacaatgag ccggtgttgg cccgctcggc
     2161 gagcgcgggc taccgggcac aaaaatatgt tcggcgccac cggttgggtg tcgcgatggc
     2221 gggagtaggg ctggtgttgc tgatcgcctt tgcggtgctc caggccattc agttacggcg
     2281 gactacgcga gagcgcaatc gtgccgaccg catcactgga ttcatgactg gcatgttcaa
     2341 ggtggctaac ccgagcgagg cgcggggaaa caccattaca gcacgcgaga tcctcgatag
     2401 agggtcgaaa gacatcgaca ctggactaag taatgatccg gaactgcagg ccaaaatgat
     2461 gtacatcatg gccaatactt actacggtct tggcctgtac tcccgtgcgc agtcactggt
     2521 cgaacgtggg atagagatcc aacggcgagt gctgggtccg gagcacccgg acacgcttcg
     2581 ttcgatgtac cgcctggcgt gcatccttcg tgacggcggt catttggcgg aagcggagaa
     2641 gctgtcccgc gagacgttgg agattcagcg gcgcgttgtc ggtctcgacc accccaacac
     2701 gctcgagtca atggccaacc tggcggtcgt actcacctac gaaggtcact atgccgaagc
     2761 ggagaagctg gaccgccaga tactagagac cgaacgacgc ttactaggcc ctgatcaccc
     2821 gaacacgctt ctttcgatga acaatctggc ggccgagttg gtggactcgg ggcgagatgt
     2881 cgaagcagag aaggtgttac acgagacact ggagattcag cggcgcgtcc tcggatccga
     2941 tcacccggac acgcttcgtt cgatgagcaa tttggcgaat acactgaggg atgaaggccg
     3001 tgacgccgaa gcggagaggc tgttccacga gacactggag attcagcagc gcgtcctcgg
     3061 acctgagcac cgcgagacac ttacttcgaa gctcaacttg gcggtcacac tttcccacga
     3121 aggtcattat gccgacgcgg agaagctgtt ccgcgagacg ctggatgttc agcggcgcgt
     3181 cctcgggccc gatcatcaga gcgcgctgga gactctcgag agcctgggga tcagcatcag
     3241 ccatgatggg caccggtatg ccgaagcaga acaactgttc cgcgaagcaa tcgagagggc
     3301 gacgaaagca aaccagccac gcatactggc ccaggcctgg tataacttcg cctgcggggc
     3361 agccgtcgct gggcgtcatg atgaggcgat caagtatttg aaccaggccg tcgataacgg
     3421 cttcgaagtc acagttgaat cgattgcatc tgaccccgac ttgaaatccc ttcgcggtaa
     3481 cgtcaccttc gaggctcttc ttaccaaaat gcgccaaggg accgccgcaa aatagcgatg
     3541 accgattacg gcggcgaccg ggttctgaac gcgcacttat ctgctgaaat cagacaattt
     3601 cgctaaacga gcaggatcac ttcaggctag attctgggcg agtgactggg tactcggaaa
     3661 ctaatcggtt cggcggataa ctgttcaaag caggccatgt tgtaacctaa gtgaaccggg
     3721 cataatgctc cgagcagcat ccgcctgtca cattggccag aaaccggctt gtgggagatt
     3781 agtcggccag cgtccggcaa ccgaattgcc accgccactt ggctgtaaaa acgcccgacg
     3841 cgtctgcg
//
LOCUS       DASRKK010000798         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_186313, whole genome shotgun sequence.
ACCESSION   DASRKK010000798 DASRKK010000000
VERSION     DASRKK010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186313"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2587
                     /locus_tag="VFC15_19120"
     CDS             <1..2587
                     /locus_tag="VFC15_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM12325.1"
                     /translation="VLGHYRLIEQIGAGGMGVVFRARDEQLQRDVAVKILPAGLFADD
                     ATRKQFRKEALAVGKLNHPNIAMAFDFGEENGVDYLVTEYIPGLNLDEMLVQQALPQK
                     TVLELGIQLASGLEAAHRENVIHRDLKPGNLRVNPDGQLKILDFGLAKLVEPIDEVAE
                     TANLNTSLSLSGTVPYMAPELLRAETADARSDIWAAGAVLYEMATGKRAFPDRQPSLL
                     IDAILHYDPVRPCLLNPEVSAALETVILKALDREPDLRYQSARELRADLGRILAGDEL
                     STETLRRTSFFEVERKARHRKAALILAAVLLAGVAVGYGVKRWWPTPSSNQQRIMAVL
                     PFDTVGQDPATSALGLGLTETLTAKLVQASDTDAIQVVSPRDLRDQGVKTAEDARREF
                     GTDFVLESSLQRSGHTIRVNCFLVDSKTHRQIAARSITVDAGDAFGLQDRVVSETLDM
                     LPAQIKPEQRRRLNVRQDTQPAAYEAYIQGRGYLQEYEKSENVDSAIAAFNQALKIDP
                     GYALAYAGLGKAYFTGFQQFYKGKPWVDNASANCERALSLNPEILEAHICLGNVFNGT
                     GKYDKAVEEFQGALRSEPSSEDALRGLADAYTNLGNFAAAESIYKKVIALRPNYWGVY
                     SWLGFFYYNQARYPEAAQMFLKATQLAPDNYRGYLALGGTYGLQGRYSDAIDALKRSI
                     ELRPSPDAYNNLGYVYTLMHRFPEAIAALEQALKIDDSDWMNWGNLADALYWSPDRRP
                     QASAKYQQAVKIGTSRLQINPRDAETLAYLANYSAMLDDRQAAFGYMQRALELAPAKG
                     EVLFRAAIVYNRFGQTDQVLAYLKKAVDVGYSRATIRDTPDFQGLQQNAQFRTLTGVS
                     QQRQR"
BASE COUNT          635 a          839 c          845 g          572 t
ORIGIN      
        1 ggtattgggg cattatcggc tgatcgaaca gatcggcgcc ggaggcatgg gcgtggtctt
       61 tcgcgcccgt gatgagcaac tgcagcgcga cgtcgcggtc aagatcctgc ccgccggatt
      121 gttcgccgac gatgccactc gcaagcagtt ccgcaaggag gcgcttgcgg ttggcaagct
      181 gaaccatccc aacatcgcca tggccttcga cttcggggag gaaaatggcg tcgattatct
      241 ggtcacggaa tacatcccgg gcctcaatct cgacgagatg ctcgtccagc aggcgcttcc
      301 gcaaaagacc gtgctggaat tgggcataca actggcgagc gggttggaag ccgcgcaccg
      361 cgagaatgtc attcatcgcg atctcaagcc tggaaatctg cgggtcaacc ctgacggcca
      421 gttgaaaatt ctcgattttg gcctggccaa gctggttgaa ccaatcgacg aggtggccga
      481 gacagcaaac ctcaacacca gtctctcgct cagcggaacg gtgccctaca tggcacccga
      541 gctgttgcgg gctgaaaccg ccgatgcccg ctccgacatt tgggcagccg gcgccgtgct
      601 ctatgaaatg gcgacgggta agcgcgcctt tccggaccgt cagccgtcgc tgctgatcga
      661 tgccattctg cattacgacc ccgtgcgccc ctgcctcctc aatcccgaag tttcggctgc
      721 gttagaaacc gtgatcctga aggcattgga tagggaaccc gatctgcgct accagtcggc
      781 gcgggagttg cgggcggacc ttggccggat cctcgcaggt gatgagctgt ccacggaaac
      841 tcttcgccgt accagttttt tcgaggttga gcgcaaggcc cgccatcgaa aagccgcatt
      901 gatcctggca gcggtattgc tagcgggagt cgcagttgga tacggcgtca agcggtggtg
      961 gccaacaccg tcctccaacc aacaacgaat catggccgtg ttgcccttcg ataccgtagg
     1021 ccaagaccct gcgaccagcg ctctcggtct gggcctgacc gaaaccctta ccgccaagct
     1081 ggtccaagcc agcgacacgg atgccatcca ggtggtctcg ccgcgcgatc tgcgcgacca
     1141 gggagtcaag accgcggaag acgctcgccg cgagtttggc accgatttcg ttctggagag
     1201 cagcctgcag cgctccggtc atacgatccg agtcaactgc tttctcgtgg attccaagac
     1261 gcatcggcag atcgcagccc gcagcatcac cgtggatgcc ggcgacgcct ttggactgca
     1321 agaccgtgtg gtgagcgaga ccctggacat gctgccggcg caaatcaagc cagagcaacg
     1381 ccgcagattg aatgtccgtc aggacactca gcccgcagct tacgaagcct acatacaggg
     1441 ccgcggatat ttgcaggagt atgagaagtc ggagaacgtc gatagcgcga tcgccgcatt
     1501 caatcaggcg ctgaaaattg atcccggtta tgcactggcc tacgccgggc tgggcaaggc
     1561 ctacttcacc ggctttcagc aattctacaa aggcaagcca tgggtggaca atgcctctgc
     1621 caactgtgag agggcgctat ccttgaatcc tgagattctg gaagcacaca tctgcctggg
     1681 taacgtcttc aacggaaccg gcaagtacga caaggcggtg gaggagttcc agggagctct
     1741 acgatcggag cctagcagcg aagacgcgct gcggggcttg gcggatgcct acaccaattt
     1801 aggaaatttc gccgcggcgg aatccatcta taagaaggta atcgcgctgc gtccgaacta
     1861 ttggggtgta tacagttggc taggcttctt ctactacaat caggcgcgct atcccgaagc
     1921 ggcgcagatg tttctcaagg ctacgcaatt ggcgccggac aactatcgtg gatatctggc
     1981 cttaggcggc acctacggct tgcaagggcg ttattccgac gcgatcgacg cattgaagcg
     2041 ctccattgaa ctgcgaccca gccctgacgc ctacaacaat ttgggatacg tctacactct
     2101 catgcaccgt tttcccgagg ccattgccgc cttggagcag gcgctgaaga ttgatgacag
     2161 tgactggatg aactggggca atctcgccga tgcgctctac tggagtccag accgacgccc
     2221 gcaggcctcg gccaagtatc agcaggccgt aaaaatcggg acgtcgagac ttcaaatcaa
     2281 tccgcgggat gcggagactc tggcatacct tgcgaactat tccgcgatgc tggatgaccg
     2341 ccaggccgct ttcggttata tgcaaagggc gctagagctg gcacctgcca aaggcgaggt
     2401 attgttccgc gcggccatcg tttacaatcg cttcggccaa acggaccagg tcctcgccta
     2461 tcttaagaaa gcggtcgatg tcggttactc tcgggccacc atccgcgata cgcctgactt
     2521 tcaaggtctg caacagaacg cgcaatttcg aaccctgaca ggggtaagcc aacaacggca
     2581 gcgctagggt tttatcctca tgccgagagg ctgcgggccg ctgttcagct tacatgttgc
     2641 accgttgatg ctaagggtgc cataaggaaa cggtgtgcct ggctgcccaa cagcgggacc
     2701 tgaagttggg ttcgaattgt tgaaactcga ctgacaattt ccgctggtgt cccagaaagg
     2761 cgaattgcaa cttcccacgc tcgcgggagg aaactgtaaa acgaaagcag cgctgttatc
     2821 ggtcagaaaa gtgacggtat cacccgcact gagtgaaacc acctccgtgg gggatccatt
     2881 aagcttctgg a
//
LOCUS       DASRKK010000799         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_774577, whole genome shotgun sequence.
ACCESSION   DASRKK010000799 DASRKK010000000
VERSION     DASRKK010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774577"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /gene="yidC"
                     /locus_tag="VFC15_19125"
     CDS             complement(<1..1401)
                     /gene="yidC"
                     /locus_tag="VFC15_19125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZM12326.1"
                     /translation="MAEYKNPQQEPGGDRRMLMVFAVTFALIIISQIFLFKDKSKQQP
                     KPAPDQTSQSNAPQSQSEAAGAPVEAISKGRAHTTANPVKSSAVTTSAANEVETVVEN
                     DVVRIVFTNRGAQAKSWVLKKYKDEDGHPLDLVNPLAVKFGLPLSLCTYDENLRSQIN
                     SALYVGSASGAITAPGELTYEYAQGDITVRKTFQFADTYVISIETVVTQNGKPVQAYP
                     MWPAAFGDQSTGPSYASAKIEYMANDKVERLAAKKVSNGNTLRGPFNWAGAQDQYFAA
                     IFLPDNPDSAAMVTLHNSISVPKDPKNPDPNSVNHYDVLGAAVGDVSGMTRERLFVGP
                     KALPVLESVHSNTAPGQMNGPDLRNVVDFGFFSLIARPLFLWLLWTHEHMASNWGVCI
                     MILTVIINLVLLPLRITSMKSALKMQKLQPQMKAIQEKYKKYPLRDPRRSEMNVEIGA
                     LYKREGANPAGGCVPLI"
     gene            complement(1404..1685)
                     /gene="yidD"
                     /locus_tag="VFC15_19130"
     CDS             complement(1404..1685)
                     /gene="yidD"
                     /locus_tag="VFC15_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZM12327.1"
                     /translation="MRQAVQFVLRCYQRVISPMLPHACRFVPTCSQYAIEAVEGHGVV
                     RGGLLGTARLLRCHPFARAGFDPVPPASVKPGYGSPDNSLGNSHPLNGT"
     gene            complement(1682..>2030)
                     /gene="rnpA"
                     /locus_tag="VFC15_19135"
     CDS             complement(1682..>2030)
                     /gene="rnpA"
                     /locus_tag="VFC15_19135"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZM12328.1"
                     /translation="ADFQRVYQSGRRQFTGNMTVFFLRRGSGPQATGAGNTPRVGFTV
                     GKVLGGAVQRNRIKRRMREAVRLSVEACDGPIDVVFNPRKSVSNVPFAELVSEVTRAL
                     RLAAQRARTGEQK"
BASE COUNT          372 a          615 c          620 g          423 t
ORIGIN      
        1 aatcagtggc acgcatccgc ccgcggggtt ggcgccttcg cgtttgtaca acgcaccgat
       61 ctcgacgttc atctccgagc gccgcggatc gcgcaatggg tacttcttgt acttttcctg
      121 gatggccttc atctggggct gaagcttttg catcttcagc gccgacttca tgctggtgat
      181 gcgcaacggc agcagcacca ggttgatgat gacggtcagg atcatgatgc acacacccca
      241 attgctggcc atgtgctcgt gcgtccaaag cagccacagg aagagcggac gggcaatcag
      301 gctgaagaag ccgaagtcca ccacgttgcg caggtcagga ccgttcatct gcccaggcgc
      361 ggtgttcgag tggaccgact ccaaaacagg gagcgctttg gggcccacga acagccgttc
      421 ccgcgtcatg ccgctgacat cgccgaccgc tgcacccagc acgtcgtagt ggttcacgct
      481 gttggggtcc ggattcttcg ggtctttagg tacgctgatc gaattgtgca gcgtgaccat
      541 ggctgcggag tcgggattgt ccggcagaaa aatggccgcg aaatactggt cctgggcgcc
      601 cgcccagtta aacggtccgc gcaaggtgtt gccgttgctc accttcttgg cggccagccg
      661 ctccaccttg tcgttagcca tgtactcgat cttggccgaa gcgtaggaag gacccgtgct
      721 ctgatcgcca aaggccgccg gccacatggg atacgcctgc acaggcttgc cattctgcgt
      781 gacgacggtt tcgatcgaga ttacataggt atcggcgaac tgaaaggtct tgcggacggt
      841 gatgtcgcct tgcgcgtact cgtaggtcag ctcgcccggg gccgtgatgg cgccgctggc
      901 gctgcccaca tagagcgccg aattgatctg gcttcgcagg ttctcgtcat acgtgcagag
      961 actaagcgga agcccgaact tcacggccag cgggttcacc aggtcgagcg gatgtccgtc
     1021 ttcgtccttg tatttcttca gcacccacga tttcgcctgc gcgccgcgat tggtaaaaac
     1081 gatgcggacc acgtcgttct cgacgacggt ctcgacttcg ttcgcagcag acgtcgtaac
     1141 cgcggagctc ttaacagggt tggcggtggt atgagcccgc cccttggaga tggcctccac
     1201 cggtgcgccc gcggcttcgg actgggactg gggcgcgtta gactgcgagg tttggtcggg
     1261 agccggcttt ggctgctgct tggatttgtc tttgaagaga aagatctggc tgatgatgat
     1321 cagcgcaaag gtgacggcga acaccatcaa catgcggcga tcgccgccgg gttcttgctg
     1381 tggattttta tactcggcca agtttaagtt ccgttcaagg ggtgagaatt ccccagcgaa
     1441 ttatccggag atccgtaccc tggtttcacg ctcgctgggg gcacgggatc gaatcccgcg
     1501 cgcgcgaagg gatggcagcg cagcagccgt gcggtgccca gcaggcctcc gcgaaccacg
     1561 ccgtggccct cgacagcctc gatcgcatat tgcgagcagg tgggaacgaa gcgacaggca
     1621 tgtggcagca tgggtgagat cactcgctgg tagcaccgca atacgaattg caccgcctgc
     1681 ctcatttttg ttcccctgtc cgggcgcgtt gtgcggccag ccgcaaggcg cgtgtcactt
     1741 cgcttaccaa ttccgcgaag ggcacattgc taacgctttt ccgcggatta aacaccacgt
     1801 ctatggggcc atcgcaggcc tcgaccgaga gccgtaccgc ttcgcgcatc cgccgcttga
     1861 tgcggttgcg ctggacggct cctcccaaaa ccttgcccac ggtgaatcct acacggggcg
     1921 tgttgcccgc cccagtcgct tgcgggccgc taccacgccg cagaaagaac accgtcatgt
     1981 ttcccgtgaa ctgccggcga ccgctctggt acacccgctg aaaatccgcg
//
LOCUS       DASRKK010000800         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_383120, whole genome shotgun sequence.
ACCESSION   DASRKK010000800 DASRKK010000000
VERSION     DASRKK010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383120"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..817
                     /locus_tag="VFC15_19140"
     CDS             254..817
                     /locus_tag="VFC15_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12329.1"
                     /translation="MARKELGKAKRRVAKTVLRLPDLDQAKSAALNSLSSIDAQRGYR
                     HAIEEFIEWYCSEPRLSFSKAVVLRYRIHLESRHLAPGTVNLRLGAVRRLAYEASDCD
                     LLSPDLAAGIRRVKGVKKIGVRLGNWLTPEQSQQLWNTPDGNSLKGKRDRALLAILLA
                     CGLRRHEAVNLDFATSSSGKTTGPSLT"
     gene            complement(936..2969)
                     /locus_tag="VFC15_19145"
     CDS             complement(936..2969)
                     /locus_tag="VFC15_19145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZM12330.1"
                     /translation="MRKQVLVFGAILVMAPPAATAEDWHLHGGTQTDWRFSPLDQINE
                     RTVSRLGLAWSMELGTSRGLEATPIVEDGVVYTTGAWNMVFAFDARTGQMKWSYDPKV
                     PRERAYFFCCDVVNRGVAVHNGKVYEGTLDGRLIALDQRTGARIWSVQTTDPAKAYSI
                     TAAPLVANNRVIIGNAGSEYGVRGYITAYDAETGKQVWRFYTVPGDPNKGSESKAMEI
                     AAKTWHGKEWWKAGGGGSPWEGMSYDPDLDLLYFGSGNASTWYRALRGQGDGLYTACI
                     LAVKASTGELAWYFQTTPGDSFDYDATQPLVQANLMIGGRLRKVVLQANKNGFFYVLD
                     RQTGEFLSAAPFVSGITWASGVDPRTGRPIELPGVADANPKLLSPEPNGAHNWNPMAF
                     HPATGLVYFPARVGTQMVHVPDKKWKYDPNRDNVGIDGNYEGPLFAKMISMPPPTGEL
                     LAWDPVAQKAVWRAKYPVLDGGGVLATGGNLVFQGRADGIFAAFRATDGEEVWQFDAA
                     TGIMAPPVTYTVDGAQYVTVMAGWGGSAGLMNTPAVGAAKPGWGRILTFALDAKAALK
                     APPFGHTGPPLPAITAKQDPQIVHTGALLFNSHCFGCHGLNAVAGPLPDLRYSTKETL
                     DSFAGIVLAGARVSDGMPSFGKILSAKDVAAIRAYVIARAQETAKPAQASQRR"
     gene            complement(2905..3327)
                     /locus_tag="VFC15_19150"
     CDS             complement(2905..3327)
                     /locus_tag="VFC15_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12331.1"
                     /translation="MDDSDLCQPFACPDRLGAVVSKQYLLGTEAPFIVVTDGPDRTLF
                     TALDNLIAEHRVPVMMAISIGNGSGDAQGKVRSSDEPCPPNFPFARDSFTMVKVRTLF
                     AVFSAILVHTLFEGTPTHAEAGTRIRCDSCHGAPGCNR"
     gene            3356..>3782
                     /locus_tag="VFC15_19155"
     CDS             3356..>3782
                     /locus_tag="VFC15_19155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM12332.1"
                     /translation="MLLSSGDKIGQYEILAPIGAGGMGEVYRAKDLRLGREVAVKVLP
                     AGMAHNDDRLARFQREARAVAALNHPHIVTIFSVEEAGGVHFLTMELVDGQPLDRLIP
                     ESGLPIGQIVEIASALADALVAAHEKGIVHRDLKPANVMV"
BASE COUNT          723 a         1149 c         1161 g          749 t
ORIGIN      
        1 tgatcgcaag aagacgttcg cagaattatg ccgagaagat caattgctgc gaccgcccca
       61 gccacgtccc atcgcaccgc cgaccaaccc atccggcggc actcggcata atccggcact
      121 cggcataagg ccggataagt cgcgcagcgt ttacctatat ccccacgcgc ctcgtgttac
      181 cccgtccgta ttacgcgcga cccgtccgtt gaccgggcca tcatcgttct acggtcaagg
      241 agggccgtgg cgcatggcac gaaaggagtt gggcaaagcg aagcggcgcg tagcgaaaac
      301 ggtgttgcga ctgccggact tggaccaggc gaagtcggcg gcgttgaaca gcctcagttc
      361 gatcgatgct caacgaggct accggcacgc catcgaggag ttcatcgaat ggtactgctc
      421 ggagccgcgg ctctcgttca gtaaggcggt tgtgcttcga tatcgcatac acctggaatc
      481 tcggcatctg gcacccggca ccgtcaatct ccgactcggc gcggtgcggc gcctcgctta
      541 cgaagcgtcc gactgcgatc tgctcagccc agacctggcc gccggcatac gacgcgtcaa
      601 gggagtcaag aagatcgggg ttcgccttgg gaattggctg actccggaac agtcccaaca
      661 gctctggaat acacctgacg gcaattcgct gaagggcaaa cgggatcgcg cactcctggc
      721 gatcctgctg gcgtgcggcc tgcgacggca cgaagcggtg aacctggact tcgccacatc
      781 cagcagcggg aagaccactg ggccatcatt gacttgaaag gcaaggccgg ccacacgagg
      841 acggtgccgt tgcggggctg ggtcaaggcc gcgatcgatg agtggcttca ggctgccaat
      901 ctgagtgccg gcaaggctcg cacggaatca cacccctacc ttcgttgcga agcctgcgcc
      961 ggctttgccg tttcctgagc tcgcgcgatc acatatgccc ggatcgctgc cacatccttt
     1021 gcggaaagga tcttcccaaa cgagggcatc ccatccgaaa ctcgcgcacc agcgaggacg
     1081 atgcccgcga acgaatccag tgtttccttc gtcgaataac gcaggtcggg cagcgggccg
     1141 gcgacggcgt ttagcccatg acagccgaaa cagtggctat tgaatagcag cgctcctgtg
     1201 tgcacgattt gaggatcctg tttcgccgtg atagcgggca gcgggggccc cgtgtgtccg
     1261 aatggcggcg ctttcagcgc cgctttcgcg tcaagagcaa aggtgagaat gcggccccag
     1321 cccggcttcg cggcgcccac cgccggtgta ttcatgagcc ctgctgagcc gccccatccg
     1381 gccatcacag tcacatattg cgctccatcc actgtgtagg tgacaggcgg tgccattatg
     1441 ccggtcgcgg cgtcgaattg ccagacctcc tccccatcgg tagcgcgaaa cgcagcgaag
     1501 atgccgtccg cgcgaccttg gaagaccaag tttccccctg tggccaacac gccgcctccg
     1561 tcgagaacgg gatacttggc gcgccacaca gccttttgcg ccaccgggtc ccacgccagg
     1621 agttcgccgg ttggcggggg cattgatatc attttcgcga aaagcggtcc ttcataattg
     1681 ccgtcgatcc ccacgttgtc ccgattcgga tcatatttcc acttcttgtc gggaacgtgc
     1741 accatctgag tgcccactcg cgccgggaag tagaccagtc cggtagccgg gtgaaacgcc
     1801 atcgggttcc agttgtgagc tccatttggt tcgggagaca agagctttgg gttcgcatcc
     1861 gcgacgcccg gcagttcgat gggccgtccg gttctggggt ctactccgct cgcccacgtg
     1921 atgccgctca cgaaaggcgc ggcggaaagg aattcgccgg tttgacggtc gagcacgtag
     1981 aagaagccgt tcttgttcgc ctgtaggacc accttgcgga gtcggccgcc gatcatcaga
     2041 ttggcctgta cgagcggttg agtcgcatcg taatcgaagc tgtcgccggg cgtggtctga
     2101 aaataccagg cgagctcgcc ggtgctggct ttgacggcga ggatgcaagc cgtgtagaga
     2161 ccgtcgccct ggccgcgcaa cgcacgatac cacgtggacg cgttgccgct tccaaagtac
     2221 agcaggtcga ggtccggatc atagctcatc ccttcccagg gggagccgcc tccgcccgct
     2281 ttccaccact ccttgccgtg ccaagtcttg gcggcgatct ccatcgcctt cgattcggat
     2341 cccttgttcg ggtctccggg gacggtgtag aaacgccaga cctgcttgcc tgtttccgcg
     2401 tcgtacgccg tgatgtagcc gcggacgcca tactcggagc ctgcgttgcc gatgatcact
     2461 ctattgttcg cgacgagcgg cgcggcggtg atggagtagg ccttggctgg gtcggtggtt
     2521 tggacgctcc atatgcgggc tccggtgcgc tggtcgagcg cgatgagtcg cccgtcgagc
     2581 gtgccttcgt agactttgcc gttgtggacg gctaccccgc ggttgaccac atcgcaacag
     2641 aaaaaatagg ctcgctcgcg gggaaccttg ggatcgtagc tccatttcat ctgtcctgtt
     2701 ctggcgtcga aggcgaacac catgttccag gctccggtgg tatagacgac gccatcttcc
     2761 acgatgggcg tggcttcgag tccgcgagag gtcccaagtt ccatgctcca cgcgagaccg
     2821 agccgagaaa cggtccgttc attgatctgg tcgagtgggc tgaagcgcca gtcggtttgc
     2881 gtgccgccgt gtaagtgcca gtcctcagcg gttgcagccg ggggcgccat gacaagaatc
     2941 gcaccgaata cgagtacctg cttccgcatg tgtcggggtc ccctcgaata gagtgtggac
     3001 caggatagca gaaaataccg cgaagagggt gcggactttc accatagtga atgagtcacg
     3061 cgcaaacggg aagttgggcg ggcaaggttc gtccgaagat ctcaccttgc cctgagcgtc
     3121 gccgctgccg ttgccgatgg agattgccat catcaccggc acccggtgct cggcgatcaa
     3181 attgtcgagt gcggtgaaca aagtgcggtc aggtccatcc gtcacgacga tgaacggcgc
     3241 ctccgtgccg agtaggtatt gtttggaaac tacagccccc agccggtcag ggcaggcgaa
     3301 tggctggcaa aggtccgagt cgtccacctc tggtagactt gcttaggcca cttccatgct
     3361 cctttcctca ggtgacaaaa tcggtcagta tgaaattctc gcgccgattg gtgccggagg
     3421 aatgggagag gtgtatcgcg ccaaagacct gcgtcttggc cgcgaagttg ccgtcaaggt
     3481 gctgcctgcc ggaatggcgc acaatgatga ccgtttggct cgctttcagc gtgaagctcg
     3541 cgcagtggcc gcgctcaatc acccacacat tgtcaccatc ttttcagtgg aggaagcagg
     3601 cggcgttcac tttcttacga tggagttggt cgacggccaa ccgctcgaca ggctgattcc
     3661 agagagcggc ctccccatcg ggcaaattgt cgagattgcc agcgcgctcg ctgacgctct
     3721 tgttgctgcg cacgagaagg gaatagtgca tcgcgacctg aagccagcca acgtcatggt
     3781 ca
//
LOCUS       DASRKK010000801         8255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1164461, whole genome shotgun sequence.
ACCESSION   DASRKK010000801 DASRKK010000000
VERSION     DASRKK010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164461"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1790
                     /locus_tag="VFC15_19160"
     CDS             384..1790
                     /locus_tag="VFC15_19160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12333.1"
                     /translation="MRLVFKLTRWCVGHNKGRMIQKLSIGEKVGYSLGDMAANFVFQA
                     MLALQLDFYTHTYGLTAGQAGTLFLVVGLAVACLNPVMGVIADRTSTRWGKFRPWLLW
                     TALPFGIIGVLTFTTPNISPAGKIIYAWTAYILLRVIYTVNNVPYASLTAVMTSDPDE
                     RTSISSYRQIAANSAGFIVASLAIPMVKFFGHGNDARGYQLTMGLLSAVSVVFFVIAF
                     LATKERIQPDPQQKASLGQDLKELFQNRPWIVLFLATLFYFMAILVRGNVMLPYFRFV
                     AGNVDLYSWFNGFGLASLLLGVACSTAVSVRVGKRRLFIASMVLSGVFSIVLLVIPPS
                     ATVAIIGAEVLRQFSFGLSGPILWAMMGDVADYGEWKTGRRASGTVTSAVVFALWAGL
                     ALGGAIAGWLLSFYGFASEAAVQSAHAQSGILLTASVYAGLAFFATAACLFFYPITRE
                     VNLEIANELAERRKTFAS"
     gene            1849..3003
                     /locus_tag="VFC15_19165"
     CDS             1849..3003
                     /locus_tag="VFC15_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HZM12334.1"
                     /translation="MLKTRTALKLAGVAGLLAIHVAMSGQAPTSLKDAYQGCFLVGAA
                     LNPAQFSGKDPRETALVKAQFNSISPENVLKWEVVHPKPDTYDFALADQYLDFGLKNH
                     MFIIGHTLVWHSQTPKWVFEHEQGNPLSRTSLLKRLHDHIQKVVGRYKGKVNAWDVVN
                     EALNEDGTLRQSPWMKIIGEDYIEKAFEYAHEADPQAQLNYNDYSLEYEAKRKGALKL
                     VKALKAKGVPINGVGLQDHVHMGWPTLNEIDTTISEFWKLGMKVMIAELDVDVLPQAT
                     SSQAAEVGLNVAAYPKLNPYPNGLPDSVQQALAKRYGDLFATYAKHCGVVTRVTLWGV
                     TDKNSWKNDWPVKGRTNYPLLFDRSGQPKPAFHAVIEAAPKNVSSISGLF"
     gene            3067..3738
                     /locus_tag="VFC15_19170"
     CDS             3067..3738
                     /locus_tag="VFC15_19170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZM12335.1"
                     /translation="MLWDMDGTLVDSEELHWRSWRETMADEGISITHDQFLSSFGQRN
                     DSIIPLWLGADAAPERVRKIERAKEDRFRRIVRDAGVLPLPGAVAWVHRLSESGWLQA
                     VASSAPRANVEAVLGEMGTASYFQAIVSAEDVRRGKPDPEVYLLAASRLGALPDRCIV
                     VEDAAAGLEGARRAGMFRIGVNRSGPHLVADLVVASLDQLEASAFDKLVPVRQEGKGD
                     SGSPS"
     gene            3802..4503
                     /locus_tag="VFC15_19175"
     CDS             3802..4503
                     /locus_tag="VFC15_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HZM12336.1"
                     /translation="MMRLEQLRKEVLEANLELVRRGLVLYTFGNVSGISRSDGLVAIK
                     PSGVPYEALSPEDMVVTDLDGNIVEGSLRPSSDLATHLELYKAFPSIGGVSHTHSEFA
                     TSWAQARKPIPCFGTTHADYFHSPVPVTDEMSSEAIAGDYERNTGTAIIQAFEDLDYE
                     NVPGVLVANHGPFTWGRDANAAAYNAVILEHIARVAYRTIAISHDAKEISGDLHDKHF
                     FRKHGAKAYYGQEKK"
     gene            4525..5946
                     /locus_tag="VFC15_19180"
     CDS             4525..5946
                     /locus_tag="VFC15_19180"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HZM12337.1"
                     /translation="MSMDTKMTMGIIVGNRGFFPGHLAETGREEVIEALTKAGMDPVV
                     LDPSQSKHGAVETRDEAKRCAELFRAQGGKIDGVIVTLPNFGDERAIADTLRLANLDV
                     PVLVQATPDTPEKMTIAHRRDSFCGKMSACNNLRQYGIPYSLTKSHTVSPDSDEFRAD
                     LAKFAAVCRVKKGLKNLRIGSIGARPTAFNTVRYSEKMLESHGISVETLDLSEVLGRI
                     QRMKDGDDRAQAKLEGIKKYVSTSGVPAEALLKMAKLAAVVDDWMKATDVAISAVQCW
                     TSIEENLGVVPCTVMSMMSNDLMSSACEVDICGVLAMHALQLASGTPSALLDWNNNYG
                     EDPNKAVCFHCSNLPKHFFNDVKMDYQAIIAGTVGIENTYGTCVGKVKAAPMSYARFS
                     TDDTSGRLRGYVGEGAFTDDALNTFGGAGVVEIPEMQKLLRYICENGFEHHVAANLSM
                     VAPAIYEATTRYLGWDMYFHEPR"
     gene            5955..7550
                     /locus_tag="VFC15_19185"
     CDS             5955..7550
                     /locus_tag="VFC15_19185"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HZM12338.1"
                     /translation="MAIVAGVDFGTLSVRVSIVDSERGLLDSAVANYPLHRKQEDPEH
                     ATQSHEDHMRALAEATREALKKSGVVGEAVEAIAIDTTGSSVIPVGKGMAPLGEYYLW
                     CDHRAKGEAAEITEAARREGLEAIQWCGGVYSSEWGFSKLLHWLRHHPEKRGEFVTAF
                     EHCDFVTATLCGISDPRQAKRSICAMGHKWMWNASLGGLPPEEFLAGVDPLLAGVREK
                     LQGEYATSEQVAGTLSAEWAEKLGLKPGIPIPVGAFDAHWDAIGAGCGTDDVVNVVGT
                     STCIIGITESPNLVPGVCGVVSGSVHPGRTGIEAGLSAVGDIFSAIATRAGTDVETLS
                     KGLERYHAGQTGLLRMTWDNGDRTVLVNPNLRGITLGWNLQSTAQDELFAAIEGTALH
                     TRVILERMAEHGVDIKRVINAGGIPQKNNVLNQVYANVLGRPALVPSKSVVSLGSAIF
                     AFLAAGTFKTIEEAQGKICPTHVTYLPDSKEQKVYEGLYAAYRKLYFAFGDPSDLMGD
                     VLPTLIRTAESVRRDAVSPSLVS"
     gene            complement(7547..>8255)
                     /locus_tag="VFC15_19190"
     CDS             complement(7547..>8255)
                     /locus_tag="VFC15_19190"
                     /inference="COORDINATES: protein motif:HMM:NF044297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12339.1"
                     /translation="SKIYAKPFDSFWCCNGTGAEEFAKLTDTIYYHDGDSIYVNLFIA
                     SEVNWPEKGLRIAQRTSFPEEQGTTLLISAAQPANVDLKLRIPFWAKTGIVKVNGHVV
                     PAFAAAGSYLVLRGPWKNGDRVELSLPMHLHAAPMPDKNTVQAVMYGPLVMAARFNEE
                     PRDNWYRHFAAEEKQEPAPTQQFKGKIEDPASWLEPAGGTLTFRTVAQSQSATFIPLS
                     SIVHERYSVYHEVEETT"
BASE COUNT         1760 a         2187 c         2585 g         1723 t
ORIGIN      
        1 agtcacaatg tttcctcaca aatggcagtt cagcacagtc ttagcacgga cgtgcacttg
       61 tacgctggca ataatcttcc tgtcaaggag tgcgagacac gcacacatgg tcgtcgcgtg
      121 gcgtcatcgt gcgatttcat cttcagatta gactgcattc ggaaatcgcg cgactccggg
      181 caccgcccgg gtgagtcata tcacggttca tgtcagggtg gtgtgataca gttcggcgtt
      241 tgaatgaatt caatctgtgc gacacgttct tgcgaacctc aagcacgtgg ttgaaccggt
      301 tgaattcgcg atcatggtcg gcagtgcagg tattatgccg gtctccagaa agtgatgttg
      361 cacgccggct agtcgaggct tgtatgcggc tggtgttcaa gctgacgcgc tggtgcgtcg
      421 ggcacaataa gggaaggatg attcagaaac tctcgatcgg cgaaaaggtc ggctattctc
      481 tgggcgacat ggccgctaat tttgtcttcc aggccatgct cgccttgcaa ctagacttct
      541 acacccacac ctatggcctt acggccggcc aggcgggaac gcttttcctc gtcgtcggct
      601 tggcggtggc ttgcctgaat cccgtcatgg gtgtgattgc ggaccgcacc agcacaagat
      661 gggggaagtt ccggccttgg ttgctgtgga cggcgctgcc cttcggcatc atcggcgtcc
      721 tgaccttcac aactcctaac atcagcccgg ccggcaagat catctatgca tggaccgcct
      781 acatcctgtt gagggtgatc tacacggtca acaatgtgcc gtatgcgtcg ctgacggcgg
      841 tcatgacctc ggaccccgac gaacggacca gcatctcatc gtaccgccag attgcggcca
      901 attccgcggg tttcatcgtc gccagcctgg ccatacccat ggtcaagttt ttcggtcacg
      961 gcaatgacgc tcgaggttac caactcacca tggggctgct ttcggctgtg agcgtcgtct
     1021 tctttgtgat cgcgtttctg gcgacaaagg agcgcatcca gccagatccg cagcaaaagg
     1081 cctctctcgg ccaggacctc aaggaactgt tccagaaccg tccgtggatc gtgttgtttc
     1141 tcgccacgtt gttctacttc atggccatcc tggtgcgcgg caacgtgatg ctgccctatt
     1201 tccgatttgt agccggtaac gtggacctct actcctggtt caatggattt gggcttgcct
     1261 ctttactgct aggggttgcc tgctccaccg cagtgtcggt ccgggtaggt aagcgccggc
     1321 tgtttatcgc gagcatggtt ctgtcgggcg tcttcagtat tgtgctgctc gtgattccac
     1381 cgagcgctac cgttgccatc atcggagctg aagtgcttcg tcaattttcc ttcggcctgt
     1441 ctgggcccat tctgtgggca atgatgggag atgtcgccga ctacggcgaa tggaagacgg
     1501 gccgccgcgc cagcggtacg gtgacttcag cggtggtatt tgctctatgg gcgggtctcg
     1561 cactgggcgg cgcaattgca ggctggctct tatccttcta cggttttgct tccgaggctg
     1621 cggtgcagtc ggcgcacgcg cagtcgggca tcctgttgac cgcgagcgtt tacgcaggac
     1681 tggctttctt cgctaccgcg gcatgtttgt tcttctatcc catcacaaga gaagtcaact
     1741 tggagattgc caatgagctg gctgaacggc gcaagacgtt cgcttcttag gcggacgggt
     1801 acgtcggaat cgccttttcg gcgggacatc tcagggcgaa gaaatcaggt gctgaaaaca
     1861 cggacggcgt tgaaattggc aggagtggcg gggttgttgg cgattcatgt ggcgatgtca
     1921 ggccaggcgc caacctcact gaaggacgca tatcaagggt gctttctggt tggagccgcg
     1981 ctgaatccgg cccagttttc cggaaaggat ccgcgggaga ctgcgctggt caaggcccag
     2041 ttcaacagca tcagccccga gaatgtcctg aagtgggaag tggttcatcc caagcccgac
     2101 acctacgact tcgcgctagc ggaccagtac ttggactttg gtctcaagaa ccacatgttc
     2161 atcatcgggc atacgttggt atggcattcg caaacgccca agtgggtgtt tgaacatgag
     2221 cagggtaatc cattgtcccg gacctcgttg ttaaagcgcc tgcacgacca cattcaaaag
     2281 gtagtgggcc ggtacaaagg caaagtcaac gcatgggacg ttgtgaacga ggccctaaat
     2341 gaagatggca cgctgcgaca atcaccgtgg atgaagatca tcggcgaaga ctacatcgaa
     2401 aaggcgttcg agtatgctca cgaggccgat ccgcaagctc agctgaatta caacgactac
     2461 tcgctcgaat atgaagccaa acgcaagggc gccctcaaac tcgtaaaggc cttgaaggcg
     2521 aaaggtgtac ccatcaacgg cgtcggccta caggaccacg tccatatggg ctggcctacg
     2581 ctgaacgaga tcgacacaac aatctcggaa ttctggaaat tgggcatgaa ggtgatgatc
     2641 gccgagctcg atgtcgacgt gcttcctcaa gccacatcga gccaggctgc agaggtcggt
     2701 ttgaacgtcg ctgcatatcc caagctcaac ccatatccca acggactccc cgattccgtt
     2761 caacaggcac tggcgaagcg ctacggagat ttgtttgcca cctatgcgaa gcattgcggt
     2821 gtagtgacgc gggtcacatt gtggggtgtc accgacaaga actcgtggaa gaacgactgg
     2881 cccgttaagg gcagaacaaa ctatcctctg ctctttgacc gcagtgggca gcccaaaccg
     2941 gctttccacg ctgtcatcga ggcagcaccg aagaacgtat catcaatcag cggtctgttc
     3001 taagcgagga agactggaag agatcgtcca gatgatggag aactccacgt gtgcgttgtt
     3061 cgcgcagtgc tttgggacat ggatggaacc ctagtcgatt cagaagaact gcattggcgc
     3121 tcgtggcggg agacaatggc agatgagggc atttctatca cccacgatca gtttctatcc
     3181 tcatttggtc aacggaatga ttccatcata ccgttatggc tgggtgccga tgcggctccc
     3241 gagcgggttc ggaagatcga aagggccaag gaggaccgat ttcgtcgcat agttcgcgac
     3301 gccggagtat tgccgctacc tggggcggta gcgtgggtgc atcgactaag cgaaagcggt
     3361 tggcttcagg cagttgcctc gtcggcaccg cgcgcgaatg ttgaggcggt tctgggggag
     3421 atgggaaccg cgagctattt tcaggccatc gtttctgctg aagatgtgcg ccgaggcaag
     3481 ccggaccctg aagtgtatct actggctgct tcgcgattgg gagccctacc cgacaggtgc
     3541 atcgtcgttg aagatgcggc cgccggtctt gaaggagcgc gtagagcggg tatgttcaga
     3601 atcggcgtca atcgcagcgg tccgcacctt gtcgcagact tggtcgtggc ctcactggat
     3661 cagctcgaag catccgcgtt cgacaaactg gtgccagttc gtcaggaagg taagggagat
     3721 tcagggtccc cgtcctgaga cagatacgtc gacaatcgcc atgtattatg gtttctccga
     3781 agcgcagggc gaccaaggag catgatgcga ctcgaacaac tgcgaaagga agttctcgag
     3841 gccaacctcg aactcgtgag acgcggattg gtgctttaca cgttcgggaa tgtaagcggg
     3901 atttcgcgat ctgacggact ggttgcgatt aagccgagcg gcgttccgta cgaagcgctg
     3961 agtccggaag acatggttgt gacagacctc gacggcaata tcgtcgaggg tagcttgcgg
     4021 ccgtcgtcag acctggcaac gcacctggaa ttgtataaag cgttcccaag cattggtgga
     4081 gtttcgcaca cacattcgga atttgcgact tcgtgggcgc aggcgcgcaa accgattcca
     4141 tgttttggca caacccacgc ggattacttt catagccccg tgccggtgac ggatgagatg
     4201 agttccgagg ccatcgccgg tgattacgag aggaacacgg gcacggcgat cattcaggcc
     4261 ttcgaggacc tcgattacga gaacgtgccg ggagtgctgg tcgccaacca tggcccgttt
     4321 acctggggac gcgatgcgaa cgcggcggca tacaacgcgg taatcctgga acacatagcg
     4381 cgcgtcgcct accgtacgat tgcaatcagc cacgacgcca aagagatttc gggtgatttg
     4441 catgacaagc atttctttcg caagcacggc gcgaaagcct actacgggca agagaagaaa
     4501 tagttctaca taaagggtgc gatcatgagt atggacacga aaatgacgat gggaatcatc
     4561 gtgggaaatc gcggattctt tcccggccac ctggcggaaa cgggccgcga ggaggtgatt
     4621 gaggcgctaa cgaaggcggg catggatccg gtggtgctcg atccatcgca aagcaagcac
     4681 ggagcggtgg agacacgcga cgaagccaaa cgatgtgcgg aactgttccg tgctcagggc
     4741 gggaagatcg acggcgtgat cgttacgctg ccgaacttcg gggacgagcg cgcgattgca
     4801 gacacgctgc ggctggccaa ccttgatgtg ccggtgctgg tgcaagcgac gccggacact
     4861 ccggagaaga tgacgattgc ccatcggcgc gatagcttct gcggaaaaat gtcggcgtgc
     4921 aacaacctgc ggcagtacgg gattccgtat tcgctgacga aatcgcacac agtcagtccg
     4981 gattcggatg aatttcgagc ggacctcgca aagttcgccg cggtctgccg agtgaagaaa
     5041 ggattgaaga acctgcggat cggcagcatc ggcgcgcgtc cgacggcgtt caatactgtc
     5101 cgctacagcg aaaagatgct ggagtcgcat ggcatctcgg tggagaccct tgatctgtcg
     5161 gaggtgctgg ggcgcatcca gcgcatgaaa gacggcgatg accgggcgca ggcgaaactc
     5221 gaaggcataa agaagtacgt ttcgaccagc ggagtgcccg ctgaggctct gctgaagatg
     5281 gcgaagctcg cagcggtggt tgatgactgg atgaaggcga cggacgttgc gatcagtgcg
     5341 gtgcaatgct ggacttcgat cgaagaaaac ctcggcgtgg tgccgtgcac agtgatgagc
     5401 atgatgagca acgacctgat gtcgagcgcg tgcgaagtgg acatctgcgg cgttctggca
     5461 atgcatgcgc tgcaactggc gtcgggtacg ccgtcggcgc tgctggactg gaacaacaat
     5521 tacggcgagg acccgaacaa ggcggtctgc ttccactgct caaacctgcc gaagcacttc
     5581 tttaacgacg tgaagatgga ctaccaggcc atcattgccg gcacggttgg cattgagaac
     5641 acctacggga cgtgcgtggg caaggtgaag gcggcgccaa tgagttatgc gcggttctca
     5701 accgatgata cttcgggcag gttgcgcggt tacgtgggcg aaggggcatt caccgatgat
     5761 gcactgaaca cttttggcgg cgccggcgtg gtggagattc cggagatgca gaagctgctg
     5821 cgttacatct gcgagaacgg attcgagcat cacgtggcag cgaacctttc gatggtggcg
     5881 ccggctatct acgaggcgac gacccggtac ctgggctggg acatgtactt ccacgaacct
     5941 cgttaggggc gcacatggca attgttgcgg gagtagattt cggaacgttg agcgtgcgcg
     6001 tctcgattgt ggacagcgaa cgcggactgc tggattcggc agtcgcgaat tatccgctgc
     6061 accggaagca ggaagacccg gagcatgcca cacagtcgca cgaagaccac atgcgcgccc
     6121 tcgcggaagc gacacgcgaa gcgctgaaga agtcaggtgt ggtcggcgaa gccgtagagg
     6181 cgatcgcgat cgacacgacg ggatcgtcgg tgattccggt cggcaaaggt atggctccac
     6241 tgggcgagta ctacctgtgg tgcgaccacc gggccaaagg cgaagcggcg gagatcaccg
     6301 aggcggcacg ccgcgaggga ctggaagcga tccagtggtg cggcggcgtg tattcgtcgg
     6361 agtggggatt ttccaagttg ctccattggc tgcgtcacca tccggagaaa cgcggcgagt
     6421 ttgtcacggc gtttgaacac tgcgactttg ttacggcgac gctgtgcggc atttccgacc
     6481 cacgacaggc gaagcgcagc atctgcgcga tgggccacaa gtggatgtgg aatgcgtccc
     6541 tcggaggcct gccgccggag gaatttctgg cgggcgtaga tccgctgctg gcaggcgttc
     6601 gcgagaagtt gcagggtgag tacgctacct cagagcaggt ggcgggaacg ctgtctgcag
     6661 aatgggcgga gaagctgggt ctgaagccgg gaatccctat tccggtgggc gcgttcgatg
     6721 cgcattggga cgcgatcggg gcagggtgcg ggaccgacga cgtcgtcaat gtagtgggaa
     6781 cgtcgacctg cattattggc atcacggagt cgccgaacct ggtgccagga gtttgtggcg
     6841 tggtgtcggg cagcgtccat ccagggcgga caggaatcga ggccgggctc tcggcggtcg
     6901 gcgacatctt cagcgcgatt gcgacgcgcg ccggaaccga tgtcgaaacg ctttcgaagg
     6961 gactggaacg gtaccacgcg gggcaaaccg gccttctccg aatgacgtgg gacaacggcg
     7021 accggacggt gctggtgaac cctaatctgc ggggcatcac gctgggctgg aacctgcaga
     7081 gcacggcgca ggatgagctg ttcgcagcga tcgagggaac cgcgttgcac acgcgggtaa
     7141 tcctcgagcg gatggcggaa cacggtgtcg acatcaaacg tgtaattaat gccggaggca
     7201 ttcctcagaa gaacaacgtg ctcaaccagg tttacgccaa cgtattaggg cggccggcgc
     7261 tggtgccgag caagagcgtg gtgagtttgg gctcagcgat ttttgcgttt ctcgcagcgg
     7321 gcacgtttaa gaccatagaa gaggcgcagg gaaaaatctg tccgacacac gtgacctacc
     7381 tgcctgattc gaaggaacag aaagtttacg aaggtctgta cgcggcctat cgcaagcttt
     7441 atttcgcctt tggcgatccc agcgatctca tgggcgacgt gctaccgacg ttgattcgga
     7501 cagccgaatc ggtgcgccgc gatgcggtgt caccttccct tgtcagctaa gtcgtctctt
     7561 caacctcgtg gtatacggag taccgttcgt gcacaatgct tgataaaggg ataaacgttg
     7621 cactctgact ttgtgcgacg gttcgaaagg tcagcgttcc cccagccggt tcgagccaac
     7681 tcgcgggatc ctcgatcttc cctttgaact gctgcgttgg agcgggctcc tgtttctctt
     7741 cggcagcaaa gtgccgatac caattgtcgc gaggctcttc attgaatctt gccgccatga
     7801 ccagaggacc atacatcacc gcttgcaccg tgttcttgtc aggcatcggt gcggcatgca
     7861 gatgcatggg gagacttaat tccacgcgat cgccattctt ccagggacca cgcaggacga
     7921 ggtagctgcc cgcagcggcg aacgctggga cgacgtggcc atttactttt acgatgccgg
     7981 tcttcgccca gaaagggatg cgcaatttca agtcgacatt ggcgggctgg gcggccgaaa
     8041 tcaacagggt agttccttgc tcctccggaa atgaggtccg ctgggctatg cggagtccct
     8101 tttccggcca gttgacttcc gacgctataa aaagattgac gtagatggaa tcgccgtcgt
     8161 ggtagtagat ggtgtccgtc aacttcgcga actcctcggc gcccgtgccg ttgcaacacc
     8221 agaacgaatc gaagggcttg gcgtaaatct tggag
//
LOCUS       DASRKK010000802         7742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_90961, whole genome shotgun sequence.
ACCESSION   DASRKK010000802 DASRKK010000000
VERSION     DASRKK010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90961"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1106)
                     /locus_tag="VFC15_19195"
     CDS             complement(369..1106)
                     /locus_tag="VFC15_19195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZM12340.1"
                     /translation="MLSDTRPPIDTPPAAARERRLLAPLWHTAIVVFVLLGASLGGSR
                     GVHPLTGHSKIPQYLWTMAWEWVLAGFVYLGIRKRMKVRELIGGRWERPEDALLDLMI
                     AAGFWLTAIVVLGAGAKLMHLDQAGKFDSMRQQLGFLIPGTTLELLVWFCLSTTAGIC
                     EEIIFRGYLQRQFAAFTSSMLAGVLLSALVFGAAHGYEGGPRMLLIGIYGLMFGLLAW
                     WRKSLRPGMIAHAWHDAISGAVLHMLK"
     gene            1164..2363
                     /locus_tag="VFC15_19200"
     CDS             1164..2363
                     /locus_tag="VFC15_19200"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZM12341.1"
                     /translation="MGLIEDVVIVSAVRTPVGKFQGSLSDVSAPQLGAIAVREAVKRA
                     GVDPASVFECIMGNVVSAGLGQNPARQAALGGGLPSTVAAMTINKVCGSSLKAVALGQ
                     QAIQTGNSEIVVAGGMESMTNAPYLLPHARAGYRLGDGKIVDALVHDGLWDAYHHYHM
                     GHTAENVAEKYGITREEQDEFALHSHRKAIAAIKECRFRDQIVPVELPSKKKGAPPHF
                     FTSDESPREDTTIEILRSLKPAFKKDGTGTVTAGNAPGVNDGASALVLMSAAKAKQLG
                     LKPMARIVAQATSGLDPAWVMMAPVETVRQLWKKTGWTNEDVDLYEINEAFSVAAIAV
                     TRELGIDLNKVNVNGGAVALGHAIGSSGSRILTTLLYEMIRRDVHRGIAALCLGGGNA
                     VGLAIER"
     gene            2503..3219
                     /locus_tag="VFC15_19205"
     CDS             2503..3219
                     /locus_tag="VFC15_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM12342.1"
                     /translation="MSSLPASKLAPAVRAENVTRHYPMGESLIRAVDGVSLTIEPGEF
                     VALLGQSGSGKSTLLNLLAGLDRPTSGSVSVLGRDLAKMSSEELARYRRNDVGMVFQS
                     FHLIPSMTITENVELPMRFAEVERTERAQRVRESLDRVGLSKRVEHRPSQLSGGEQQR
                     VSLARALANRPSLLLADEPTGNLDSRTGEDILNLIRDISLSLGMTVVMVTHERVLAER
                     FAQRLIFLADGKLVAEEVRA"
     gene            3216..4601
                     /locus_tag="VFC15_19210"
     CDS             3216..4601
                     /locus_tag="VFC15_19210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM12343.1"
                     /translation="MKSYDLFELAVRNLRQSKLRNGLTTVGISVGVASLVAMLSLGVG
                     LQQLATRRLAGSGMFDTVFVTSRQDFRGFDREDDQKAPHPEQALVLDESARVKMTKLP
                     DVTEVEPEIRVMGEVVSGGETHFGFVTGLPMSARENEAFDTLKGKFFSSDDADEAILL
                     LGFAKELNPKNPSELIGQDISLRYGERQALSQDASDRPQLSGDDTKANPAGANGDDFG
                     FSVVRKIQKLRVVGLIDQEPYGGMRTVSRGRIFLPVATTERLNMAQFTDMRSSLSGGG
                     GKTYTTLTVRVKDPAKVQGVQDAISQMGFRTFSVLDATKSLRRFFTVLDLFLGIFGSL
                     ALAVATLAIINTLVMAVLERRREIGVMKAIGASDGDVKKLFFTEAGAMGFFGGVLGIA
                     LGFAIGKAINLGTGIYLKQHQLPAEPVWIMPVWLIGAAIAFSIVVSLLAGLYPASRAA
                     KLDPVQTLKYE"
     gene            4598..5497
                     /locus_tag="VFC15_19215"
     CDS             4598..5497
                     /locus_tag="VFC15_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM12344.1"
                     /translation="MMKSRPAHISYTFRIAAFLLALSLAPFAHAEGRAECSVVNSTIL
                     ARSVRYCAYLPASFDQDKARRYPVLYYLHGLGDNEQSLLNLGGWDLIEELRRQGKIGD
                     FVVLAPSGGHTFFLNSADGKVRYEDFLTREFMPQMEKKYRVEAGRATRGITGVSMGGF
                     GALHLAFKYPQQFAAVSAQMPALIAELPPDFAAGGPGSPGSMMGDIFGNPINLAYFER
                     NNVFYYAKTDPVADLKRMKIYFDVGNNDDYGFEQGGQKLHQLLDSRGVSNEFHVYPGR
                     HDPQFVMRHFADVMQFQWKAIAK"
     gene            complement(5546..7096)
                     /locus_tag="VFC15_19220"
     CDS             complement(5546..7096)
                     /locus_tag="VFC15_19220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZM12345.1"
                     /translation="MALEQRTSASEATQPGAADKHPDIDLVAGKSRWSRFFDPTIFGL
                     FLFFAIMLPHSIKGAERAWKIALVLWLLKLAVERLRPYRLPLAAPLLTYVTLSAISTL
                     LSSDPSLSWDRMKFVCLFLIGIVIAQNLKWLSQVRWLLMLLVLSGFSAALYTGWQYTY
                     GVGVRLADFPSSSRLSELGFAPGDVITSFDGRRVHTPEQMVRAVQQSRSAAKTNVQYV
                     RGLGFHESTIAATPEDFLKSGLGTESVKLDRGKPLRAQGTLGHYVVFAEMLMQIGCMA
                     WALFLCAPRGRTGWKLLLAMAFAGITAALLATSTRASVAGLLLGCAVSLILLSSRKVR
                     IAGIITLVVILAAGTLWIQNSRRSEWVNGGDLSTHFRVLMWEDGIRLVREHPWFGVGM
                     ETVRVHYREWNIRGFIEYNVISHFHSTFLQIAVERGIPALLAWLWFCGAYFVFLGRLI
                     RRLHNQSRFACGVAVGVLAAFTAFTFTSLFHYNLGEEPLAMITFFYFGIALAVDRMTA
                     TPEALDVP"
BASE COUNT         1646 a         2317 c         2249 g         1530 t
ORIGIN      
        1 aaaaaaacgc gcaccaagtt gagtaattcg gtgacgcgtc tctcgcatcg cgaccccgca
       61 tgagtgcggt cctcaccgtc tccgttgccg cgatcggctc cgccgattgt tcatgtgcgc
      121 gaaacctgca tgaacactgg cgatttgcat cgctgcattt tctcgcaacg agattgcgag
      181 ttgctcgatt cgttccttga gcgagacagc gtccgatttc gtcgcaccaa cgcatgccaa
      241 atattttttc gctaccgacg caatttggtg gtgcggaaga cgattctcgc gacctcaacg
      301 acctgcagtg taacttcaaa actgtcgtag cggagaattt ctgtccgcgc tcgcgcgcgg
      361 caattccgtc acttcaacat gtgcagcacg gctccgctga tcgcgtcatg ccatgcgtga
      421 gcgatcattc cgggacgaag actcttgcgc caccacgcca gcagtccgaa catcaggccg
      481 taaatcccga tcagcagcat gcgcggaccg ccctcgtagc catgcgctgc cccaaaaacc
      541 agcgccgaca gcagaactcc cgccagcatc gaactggtga acgccgcgaa ttgccgttgc
      601 agatatccgc ggaagatgat ctcttcgcaa attccggcgg tcgtgctcag gcaaaaccac
      661 accagcagtt ccagcgtcgt gcccggaatc aggaatccaa gttgctggcg catgctgtcg
      721 aacttcccag cctggtcgag atgcatcagc tttgcgcccg cacccagcac cacgatggcc
      781 gtcagccaga atccggcggc gatcataagg tcgagcagcg catcttcggg tctttcccat
      841 cgaccgccga tcaactcgcg caccttcatc cgcttgcgaa tgcccagata tacaaagccc
      901 gcgagcaccc actcccacgc catggtccac agatattgcg gaatcttgct gtgaccggtc
      961 agcggatgca ctccgcgcga tccgcccagc gacgccccca gcagcacgaa gaccacgatg
     1021 gccgtgtgcc agagtggtgc gagcaaccgg cgttcgcgcg ctgccgctgg cggggtgtca
     1081 atcggcggtc gtgtgtcgga aagcaaggtg gctacatctt ataatggtga gtttgccatt
     1141 cgggatcgaa aggatttgtg agcatgggtt tgattgagga tgttgtcatt gtttcggcgg
     1201 tgcgcacgcc ggtcggaaaa tttcaaggat cgttgtcgga cgtaagcgct ccgcaactgg
     1261 gcgctatcgc ggtgcgggag gcggtgaagc gcgcgggcgt cgatccggcc agcgttttcg
     1321 aatgcattat gggcaatgtg gtcagcgcgg gactggggca gaatcccgcg cgccaggccg
     1381 cacttggtgg tggactgcca tcgactgtgg ccgccatgac catcaacaaa gtttgcggct
     1441 ccagcctgaa agcggtcgca ttgggccagc aggcaatcca gacggggaac tcagaaatcg
     1501 tggtggccgg cggcatggag agcatgacga acgcgcccta cctgttgccg cacgcgcgcg
     1561 cgggctatcg tctgggcgac ggcaagatcg tcgacgccct ggtgcatgac ggcttgtggg
     1621 acgcgtacca ccactaccac atgggccaca ccgccgaaaa cgttgccgag aagtacggca
     1681 tcacgcgcga agagcaggac gagttcgctt tgcattccca tcgcaaggcc atcgcggcca
     1741 tcaaggaatg tcgcttcagg gaccagatcg tacccgtcga gttgccgtcg aaaaagaaag
     1801 gcgcgccgcc gcatttcttc accagcgatg agtctccgcg cgaagacacc accattgaga
     1861 tactccgttc gctcaagcct gccttcaaga aggacggaac cggcacggtg acggccggca
     1921 acgcgcctgg ggtcaacgat ggcgcttctg ccctggtgct gatgtcggcg gcgaaggcga
     1981 agcagttggg actcaagccc atggcgcgca tcgtggcgca agccaccagc ggactcgatc
     2041 ccgcgtgggt gatgatggct ccggtcgaaa ccgtgcgcca actctggaag aagacgggat
     2101 ggacgaacga ggacgttgac ctgtacgaga tcaacgaggc cttctctgtc gccgccatcg
     2161 cggtgacacg cgagctcggc atcgacctga ataaggtcaa cgtgaacggc ggcgcggtgg
     2221 cgctcggaca cgccatcggg tccagcggtt cgcggattct cactacgctg ctctatgaaa
     2281 tgattcgccg cgacgtccac aggggaatcg ccgccctgtg cctgggtggc ggaaatgcgg
     2341 taggtctcgc catcgagcgt tgatctctgc ttctccaggg gcggcgttta caaatcgtaa
     2401 caagggaagg ggagcattcc ggctcccctt ttcgtaggaa tcaagtggaa gggtcgtgag
     2461 actgcgccgc agcgcaactc cgtcgcatcc aatgagacac acatgagttc gctaccagct
     2521 tccaaacttg ctccggcggt gcgcgcggaa aatgttactc ggcactatcc catgggcgaa
     2581 agcctcatcc gcgccgtcga cggagtttcg ctcaccattg aacccggcga attcgtcgcc
     2641 ctgctgggac aatcgggatc gggaaaatcg accctgctca acctgctcgc cggactcgac
     2701 cggccgacct ccggatcggt gagcgttctc ggtcgcgacc tggcgaagat gtcgtcggag
     2761 gaattggccc gatatcgccg caatgatgtc ggcatggtct tccagtcgtt tcatctcatt
     2821 ccctcaatga ccattaccga aaatgttgaa cttcccatgc gcttcgcgga agtggaacgc
     2881 accgagcggg cgcagcgcgt ccgtgaatcg ctcgatcgag tcggactgag caagcgcgtg
     2941 gagcatcgtc cctcgcagct ttccggcggc gagcagcagc gcgtatcgct ggcgcgtgcc
     3001 ctggccaatc gtccatcgct tcttctggcc gatgagccta ccggcaatct cgacagccgc
     3061 accggcgaag acatcctcaa cctgattcgc gatatcagcc tgtcgctggg catgacggtg
     3121 gtgatggtca cgcacgagcg cgtattggcc gagcgtttcg cgcagcgcct gatttttctc
     3181 gccgacggaa agctggtcgc cgaagaggtg cgggcatgaa gagctacgat ttgttcgaac
     3241 tcgcggtccg caatctgcgc cagtccaaat tgcgtaacgg cctcacgacc gtcggcatct
     3301 cggtgggagt ggcgtcgctg gtggccatgc tctcgctggg agtcggtctg cagcagctag
     3361 caacgcgccg gctcgcagga tcaggtatgt tcgacaccgt cttcgtgacc tcgcggcagg
     3421 actttcgcgg attcgaccgc gaagacgatc agaaggcgcc acatcccgag caggctctcg
     3481 tgcttgacga gtccgcgcgg gtgaagatga cgaagctccc cgacgtcacc gaagttgagc
     3541 ccgagattcg agtgatgggg gaagtggtct ccggcggcga aactcacttc gggtttgtga
     3601 ctggccttcc gatgtcggcg agggagaacg aggccttcga taccttgaag ggcaagttct
     3661 tctccagcga cgacgccgac gaggccatcc tgctgctggg tttcgccaag gaattgaatc
     3721 ccaagaatcc gtcagagctg ataggccagg acatcagctt gcgctacggc gagcgccagg
     3781 cgttgtcgca agacgcatct gaccgaccgc aactctcggg ggacgatacc aaagcgaatc
     3841 ccgccggagc caatggcgac gacttcggat tctcggtggt tcgcaagatc cagaagctgc
     3901 gtgtggtcgg cctcatcgac caggaacctt acggcggcat gcgaacggtt tcgcgcgggc
     3961 gcatcttcct ccctgtggcg acgacggagc gcctgaacat ggcgcagttt accgacatgc
     4021 gcagttcgct gagcggcggg ggcggaaaga cttacacaac gttgaccgtc cgcgtgaaag
     4081 atccggccaa agtgcaaggc gtgcaggatg cgataagcca gatgggattc cgcacattct
     4141 cggtgctcga tgccaccaag agtctgcggc gtttcttcac tgtgcttgat ttgttcctcg
     4201 ggatattcgg ctcgctcgcc ctggcagtgg ccacgctcgc catcatcaac acgctggtca
     4261 tggccgtgct cgaacgtcgc cgcgaaatcg gagtgatgaa agccatcggc gccagtgacg
     4321 gcgacgtgaa gaagctgttc ttcacggagg ccggagccat gggtttcttt ggcggcgtgc
     4381 tgggcatcgc actgggcttc gccatcggca aagccatcaa ccttggcacc ggaatctacc
     4441 tgaagcagca ccaattaccg gctgagccag tgtggatcat gccagtgtgg ctgatcggtg
     4501 cagccatcgc cttctccatc gtggtgagcc tgttggcagg tttgtatccc gcaagccgcg
     4561 ccgccaaact cgatcctgtg cagaccctca agtacgaatg atgaaatcca ggccagccca
     4621 tatttcttac acctttcgca tcgcggcttt cctgcttgcg ctatcgctgg ctcccttcgc
     4681 ccacgccgaa ggacgcgccg aatgttcggt cgtcaactca acgattcttg cgcgctcggt
     4741 gcgctactgc gcgtatcttc cggcgagttt cgatcaggac aaggcgcgcc ggtatcccgt
     4801 gctgtactac ctccatgggt taggtgacaa cgaacagtcg ctgctgaatc ttggcggatg
     4861 ggacttgatc gaagagctgc gccgccaagg caagatcggc gacttcgtgg tgttggctcc
     4921 aagtggcggc cacacatttt tcctgaactc agccgatggc aaggtgcgct acgaggactt
     4981 ccttaccagg gaattcatgc cgcagatgga aaagaagtac agggtcgagg ccgggcgcgc
     5041 gacccgcggt atcactggtg tttccatggg tggctttgga gcgctgcatc tggcatttaa
     5101 gtatccgcaa cagttcgcag ctgtgagcgc gcaaatgccg gccctcatcg cggagcttcc
     5161 cccggacttt gccgccggag gaccgggctc gcccggttcc atgatgggcg acatattcgg
     5221 caatcccatc aacctcgcct atttcgagcg caacaatgtt ttctactacg cgaagaccga
     5281 tcccgtcgcg gatctcaagc gcatgaagat ttacttcgat gtgggaaaca acgatgatta
     5341 cggattcgag cagggagggc agaagctgca tcaactgctc gattcacgtg gcgtctcaaa
     5401 tgagtttcat gtctatcctg gccgccatga cccccagttc gtcatgcgcc actttgcaga
     5461 cgtcatgcaa ttccagtgga aggcgattgc caagtagccg ccaggaagtt ccgccggttt
     5521 ctccgctttg actcaatcag tagatttagg gaacatccag cgcttcaggg gttgcggtca
     5581 tccgatccac agccaaggca attccgaagt agaagaaggt gatcatcgcc agcggttctt
     5641 ctcccaggtt gtagtggaag agcgatgtga aggtgaacgc agtgaatgcc gccagcactc
     5701 ccaccgcaac cccgcatgca aaccggctct gattgtgcag gcggcgaatg agccttccca
     5761 gaaacacaaa atacgcgccg cagaaccaca accacgccag cagcgcaggg atgccgcgct
     5821 ccaccgcaat ttgcagaaac gtggaatgga aatgactgat cacgttgtac tcaatgaatc
     5881 cccgaatgtt ccactcgcga tagtgcacac gtacggtttc catgcccacg ccaaaccatg
     5941 ggtgttcgcg taccaggcgg atgccgtctt cccacatcaa caccctgaaa tgtgtactga
     6001 gatcaccgcc gtttacccac tcgctcctgc gcgagttttg aatccataga gttccagccg
     6061 caagaatcac aaccagcgta atgattcccg cgatccgcac cttgcggcta ctcagcagga
     6121 tcagagacac cgcgcacccc agcaagagtc ccgctactga agcccgggta gaagtcgcaa
     6181 gtagcgcggc cgtgatgccg gcaaaggcca tggccaaaag cagcttccat cccgttcggc
     6241 cacggggagc gcacagaaac aacgcccacg ccatgcaccc aatttgcatc agcatctcgg
     6301 caaacaccac gtagtgcccc agcgtaccct gcgcccggag tggcttgccg cggtcgagct
     6361 tcaccgattc tgtcccgagc cccgacttaa ggaagtcctc gggagttgcc gcgattgtgg
     6421 actcgtgaaa tccaagcccg cgaacatact gcacgttagt tttcgccgcc gaccgactct
     6481 gctggaccgc gcgcaccatt tgctcgggcg tgtgtacccg gcgtccatcg aacgacgtga
     6541 tgacgtcgcc gggagcgaat cccaactcgg acaatcgcga cgacgacggg aaatccgcca
     6601 gccggactcc gactccatag gtgtattgcc agccggtgta aagcgcggcc gagaatcccg
     6661 agagcaccag caacatcagc aaccaacgca cctgtgatag ccacttcagg ttctgcgcaa
     6721 tcacaatgcc gatcaggaac aggcagacaa acttcatgcg gtcccagctt aaggagggat
     6781 cggaggagag cagggtagag atcgcgctca gcgtcacgta ggtcagcagt ggtgcggcca
     6841 ggggcagcct gtacggccgc aagcgctcga ctgctagttt tagcagccac aggaccagcg
     6901 caatcttcca cgcgcgctcc gctcccttga tcgagtgcgg cagcatgatt gcgaaaaaca
     6961 gaaatagtcc gaagatcgtg gggtcgaaga aacgtgacca acgtgacttg cctgcgacca
     7021 gatctatgtc gggatgtttg tccgctgcgc cgggttgtgt tgcttcggaa gcggatgtac
     7081 gctgctctag agccatcgga aaaatttaac tacgcctatc ttacccgcaa aactcaaagt
     7141 attgctgcag ctactcatcg aatgacgacg tccgatactc cgcgatttct cgactgccgc
     7201 acgctatcgc tcgatccgcc gctgatcgaa actcttctgc ctgagacgtt ttgctttgag
     7261 cgcgaataac ggccgccacg aacgtggctc cagtgttcag caggagactg ctgttcacgt
     7321 cggtggcctt gttacgagag agcggagcga aaggttaaaa aatcggtatt gtagtttccg
     7381 tttgttgata aggaagagac acaagcgcga ttcgcacctc agtgccatac caccagccgt
     7441 gtggaacagt aggtcgtgat gctgcgtgta cgcctcgcat ttggcagaat agtgctggac
     7501 aaaacggtgt acaatttcct ttataagctg aacgcttcgc actctggcac cctatgtctg
     7561 attccattgg aggctcgaag taaccgcccc ctgtacgatt ccagacgctc tttgtcgaga
     7621 acgcctattc gactaagacg aaatagaaaa accaggacct ggtaaaacgt gttcgatcga
     7681 cgttccaagt tactcagcac cgtgacactg ctgcaagact tggcagtgac ggctgtggcc
     7741 tt
//
LOCUS       DASRKK010000803        12077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_871531, whole genome shotgun sequence.
ACCESSION   DASRKK010000803 DASRKK010000000
VERSION     DASRKK010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871531"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1152
                     /locus_tag="VFC15_19225"
     CDS             205..1152
                     /locus_tag="VFC15_19225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM12346.1"
                     /translation="MKIPSLAKSTILGVCSLTLGLAISLALPAAASTVYGTNVSSTQL
                     QSIDTSTSIVTSIFSTPGTPEGLTFDGANQILYTTANTGQLALYNTSTHSNSILSTFG
                     TNQNGAYVTLDPSGTSALFGVTGVGIDRINLSTQAVSPLLSFADPRGLAYDNAGHLFA
                     ILGPNQLSQINPTTGAIMNSIALPFTGSAGASGLAFDPVSGNLFLTDDANNLTVRGLY
                     RVGTNLSSATLLAGAPFLFANGLTADGHGNLYIAEANHLDEYNIAGDVLTTGTIDSGL
                     FDVALQPAQMTGTPEPGSLILLGTGLVGLAGTLRRRIRI"
     gene            1490..2113
                     /locus_tag="VFC15_19230"
     CDS             1490..2113
                     /locus_tag="VFC15_19230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM12347.1"
                     /translation="MDAKRNSAKASQPGEGKATQMDGPDLESVIERAQGHDAEALGEI
                     YRLYARRVFGLCRYMLDSRESAEDATSEVFLKLQRSIESYDGSVPFPRWLLRVTGNQC
                     IDVLRRRKRGRQVIVEVEEGVAVIEAASPEPSPLGAVLGAEERAQVRDAIARLPENYR
                     VPLVLRYYGELSYDEIAQQLDLKRDYVAALLFRAKQELRRKLGHWSK"
     gene            2116..3726
                     /locus_tag="VFC15_19235"
     CDS             2116..3726
                     /locus_tag="VFC15_19235"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM12348.1"
                     /translation="MECYSEETVAIFVDGELEAEEAQRLRDHLSTCRRCRQLLDALRA
                     ENRVLSESLQELPEEAASPEGFSPAPRSFAWGDVAIVTAVLALTSVVAFWISELSIPQ
                     ALQWLNPFSVSGGTNLVFNFSYYFANGGTAMLDEYAAVIGKIFLVLLVGGSALLLGRR
                     WRLRQPGLRLMMVLLALSLPGFALERRHSEIVTVRANETVDDTLLASGNIVRVDGVVN
                     GDLLAFGGTVEVRGTVKGDLVSFAKRIVVTGTVEGNIYNASNSLELDGQLSHSIYALV
                     QSLRVGDRGHVGEGLVAGAGDVSLEGEVTRSVTMFAGNADVSGSVGRELTMAGDNLTL
                     TNTARIGGNLSARVHELKNVHIADGATITGTRDIQQRVRESHFTRPRFYFFQAVWLAA
                     AMLVGWLGLVLFPGFFQASTHAVGSGWRSLGLGFAVLAGVPLAMIVTAITLVGLPASL
                     MLLMVYLAAIYLAKIWVGAFLGQMLLKPTGVTKSDWLLGLLVGLLILTVVGYIPYLGG
                     LVHFGVVCLGLGAFAWQLYRISRPAIAA"
     gene            4183..4389
                     /locus_tag="VFC15_19240"
     CDS             4183..4389
                     /locus_tag="VFC15_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12349.1"
                     /translation="MPTRYRVVRVKDVEPETFIIVGIDFDEHTIKSTSKAMSEAALRA
                     HLREAGASADETTTWIEQGRAYPE"
     gene            4415..4807
                     /locus_tag="VFC15_19245"
     CDS             4415..4807
                     /locus_tag="VFC15_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12350.1"
                     /translation="MSVTDILLAPMQGAEHREVGGVQLDTVAAGNSRVKRVIYPVGFR
                     WSKNMKPLVGTDRCMHAHVGFLARGQIHMEFADGCVLEFRAPQVIAIEPGHDGWVVGD
                     EPAVVIEFDFGSDTVRNLGMPEEHSHGA"
     gene            complement(4831..6249)
                     /locus_tag="VFC15_19250"
     CDS             complement(4831..6249)
                     /locus_tag="VFC15_19250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZM12351.1"
                     /translation="MSPEAQVLAMLRGQLIEICFGTTFIVIGLAAVAAAAIRRRTGVS
                     AIVWMGIWSAAYGILGLSGTPAFVAALPGWLQTAAPYVRTGFTYLMVVIATMAWRELS
                     LGKVRHILGALSFAGLAIGVPGFAYFVFAGSPDKLMPWNNLLAVVDLLVLLTVILVPS
                     LSRKYLVLPGRGALAVGSIVFVAEAVFVNLSRPFGHETARVWDSLAFAVLLFSFGYVA
                     LQMVFGNERRLLSIENELEIARQLQFSILPTSVPELRSLRVAANYLPMTAVAGDFYEF
                     LAVDQHRAGFLVADVSGHGVPAALIASMIKVAAQSVTAWADDPGEVLRRLSGILSGQL
                     RGQFVSAAYLWIDTEAHRARYSAAGHPPLLCWRASTGELQRIESNGMLFGVMPDAEYP
                     VCDVALETGDRVLMYTDGVVEPENAAGESFGDLKLEQVVRGNQLCTASELSEHLLAEV
                     RNWQPASMTQQDDITLLVIDVL"
     gene            complement(6565..6840)
                     /locus_tag="VFC15_19255"
     CDS             complement(6565..6840)
                     /locus_tag="VFC15_19255"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM12352.1"
                     /translation="MTMIDVWVEVDGNSAALALHEAGKKLDSAGGDAFLDFSAVRRID
                     SSGLRALGDFAGVADEKGVKVTLRGVNVDIYKVLKLARLSSRFSFVN"
     gene            complement(6837..9485)
                     /gene="adhE"
                     /locus_tag="VFC15_19260"
                     /gene_synonym="adhC"
     CDS             complement(6837..9485)
                     /gene="adhE"
                     /locus_tag="VFC15_19260"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF010378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HZM12353.1"
                     /translation="MSDKTATVITDERIEYLEGMVRQAKTAAAVFTQYTQEDVDRIVK
                     PMVLAGLEQAQYLARLAIEETKLGVMEDKAIKNMVATEFVYNYVKDKRTVGQIAEYPE
                     RNLVEMAEPIGVIFSLLPITNPTSTVLFKCIMAIKTRNAVIFSPHPAAGHCCYEAVRI
                     MYETAVKHGAPEGVFTCLESHNLQDNDYMMHHKDVGLIDATGGPGMVKAAYSSGKPAL
                     GVGPGNTPVYLEKSADLDMAVVDIITSKTFDNGTICASEQTVVIDDEIYDIVLEKFAE
                     LGSHICTEKETELLGRTVIDPKTGFMQSMAVGQKATSIARMIGLKVKPKTKLLIAPIL
                     GVGRGYPLSVEKLFPVLAVYRAKSLDEALKVCIDVNYAGGMGHTAVVFSRNDEVIRKF
                     GDLINAGRIIVNSPGSIGALGGVYNDMVPTFSFGCGTGGGNSTTDNINVFHYLNIKRV
                     ARRTQAHMWFRVPNEIYFNMNAVENLRKFKSRSTVIITNPAMEQIGHVDTVRRNLPPE
                     TLVHVSVIPDAEPEVKVIMHGVEGLNFYRADQIIALGGGSVIDAAKIMKLKYESPEAD
                     LEELAAPFLDLRKRVVQYPTEKINHSRLVAISTTSGTGSEVTPFAVLTDREHGRKVTL
                     ADYSLTPEVAIVDPQFVMSMPKGLTADTGIDCLTHALEAGVSIYASPYTDSNAFQAIR
                     MAFKYLPISYAQPNDEEARTMMHNAACIAAIAFSNASVGVNHALAHALGARFHIAHGR
                     ANALMLPHVIAYNAGVPTKFMPSPNQRGYIAHKKYAMMAELLGLGGNTVEEKVQNLVI
                     AVEQLLDQLAIPRSIAELGISKEDFERAMPELVKNAFDDPSGRSNPRMPLLSELVELF
                     WSAYNGRGPARIKEASQQRREDKVLV"
     gene            complement(9853..11718)
                     /locus_tag="VFC15_19265"
     CDS             complement(9853..11718)
                     /locus_tag="VFC15_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM12354.1"
                     /translation="MDPSPSNPDLNAFLSARDFLLQHRTDYELVYEKFRWPQLTQFNW
                     ALDYFDGFARGNQKTALWIVEENGSELKLSYDQMSRRSNQVANFLRRIGVRRGDRIIV
                     MLPNVVAMWEVMLAALKLGAVVIPSATLLTPDDLKDRLVRGQARHVIANAAFTGSFSE
                     LPGEYSRIVVDKPVNGWISYWDAYREDDTYAPETATKPIDPALLYFTSGTTAEPKLVM
                     HTHQSYPVGHLVTMYWIGVRPDDIHLNISSPGWGKHAWSSFFAPWNAGATILVHNYAR
                     FDAKKTLETISRYHVTTLCAPPTVWRLLVLEDLAAYPVQCRELVGAGEPLNPEIIEKV
                     RQSWGVTIRDGFGQTETVLVIGNFPGQTVKPGAAGKASPGHEIVLLDADGNEAADGEI
                     CMRLNPKPISLMAGYADDPERTVKVMEGGCFHTGDIASRDADGYYTYVGRDDDVFKSS
                     DYRISPFELESALIEHPAITEAAVVPAPDAIRWNVPKAYITLNPGVFPSAAVAHEIFA
                     FIRKRLGAYKRIRRIEFAELPKTISGKIRRVQLRTAEHARRAVGARREFEYWEEDFHE
                     LIQAQAYRFYEERGCEHGHDLEDWLRAESEITGPKIRRATAEPVATEARVKAAAA"
BASE COUNT         2476 a         3509 c         3548 g         2544 t
ORIGIN      
        1 catatccctc cctgtcagct gcaaagaatt ttcgatcggc tgacaatttt caccccgaaa
       61 cctcgcgtta cttgatgtag gcgcttgcgg atgcggtggt ctgaaaggac gcaccgcata
      121 gcactgtccc gtgactggcc gagcggggtg acaacaagtc tcggaaaccg agacttgaat
      181 tggaagggaa aaaggagacg aaccatgaaa ataccgagct tagccaagtc cacgatcttg
      241 ggagtatgtt cgctgactct tgggttggcg atctcactgg cgctaccggc agcggcgagt
      301 accgtgtacg gaaccaatgt cagctctact caactccaga gcattgacac cagcacctcg
      361 atagtgacgt cgattttcag cacccccgga acgccggaag ggctgacctt cgacggagcc
      421 aaccagatcc tctataccac cgctaatacg gggcaactgg cgctctataa cacgagcacg
      481 cacagcaact ccattctgtc tacgtttggt acgaaccaga acggggcgta cgtgacgctt
      541 gatccgagtg gaaccagcgc tctgttcggt gtgaccggag taggcattga ccgaatcaat
      601 ctttcgacgc aagcggtaag cccgctgctg agctttgccg atccgcgtgg cctcgcttac
      661 gacaatgcag gacacctctt tgcgattctg ggtccgaacc aactctctca gatcaatccc
      721 actactggcg cgatcatgaa cagcatcgca ctgccgttta ccggtagtgc cggcgcaagt
      781 ggcttggcct ttgatcccgt tagcggaaat cttttcctga cggatgacgc caacaacctt
      841 accgtgcgtg gactctacag ggtgggcacc aacctctcca gtgccactct gttagcgggc
      901 gctcctttcc tgtttgcgaa cggtcttacg gcagacggtc acggcaacct ctatatcgcg
      961 gaggccaatc acctggatga atacaacatt gcaggcgacg ttctgacgac tggaacgatc
     1021 gactccggcc tcttcgatgt ggctctgcaa ccggctcaga tgacggggac accggaaccc
     1081 ggtagcttga tccttctggg aacgggactg gtcggccttg cgggtacgct gcggcgtcga
     1141 atcagaatct gatcatttct gcgtggttga cggacggatc gggccggggc tacaacgctc
     1201 tggccttttt catgctcgct gtccagaatc ctcagcggtg tgcagagtgc gtttcgatcc
     1261 ggagcggcgg ggtgtgggag tggtccatag tcgcagagac ggccgattcg tccagtccag
     1321 gatgaaagga ttgttgcgaa tgccccaata ggagcgtttc gcgccatcgt gatacttcca
     1381 agctggctgg atgtcgcggt tcctcatcga tcagtgggtg aagactagcg cctgcatccg
     1441 ttatccctaa aatttgtgta actttgttcg atccgcagcg tactgcttta tggacgccaa
     1501 gagaaacagt gcgaaagcat cgcagcccgg agagggcaag gccacgcaaa tggacgggcc
     1561 ggatctggaa agcgtaatcg agcgcgcaca aggccacgat gccgaggcgc tgggggagat
     1621 ctatcgcctc tatgcccggc gtgtgttcgg cttgtgccgc tacatgctgg actcacggga
     1681 gagcgcagaa gacgccacca gcgaggtctt cctgaagctg cagcgctcga ttgagagcta
     1741 cgacggttcg gtcccgttcc ccaggtggct gctgcgggtg actggcaacc aatgcattga
     1801 cgttttgcgg cgccggaagc gtggacggca ggtgattgtg gaagttgaag agggagtcgc
     1861 ggtcatcgag gcagccagtc cggagccgtc gccgctgggt gctgtcctgg gcgcggaaga
     1921 gagggcgcag gtacgggatg ccattgcgcg cctgccggag aactatcgcg tgccgctggt
     1981 cctgcgctat tacggcgaat tgagctacga cgaaatcgca cagcagttgg acctgaaaag
     2041 ggattacgtg gcggcgttgc tatttcgggc caagcaggag ctgcgccgga aactgggcca
     2101 ctggagcaag tgatcatgga atgttattcg gaagagaccg tcgctatctt cgtggatggt
     2161 gagctggagg ccgaggaggc gcagcgcctg cgcgaccacc tctccacgtg ccggcgctgc
     2221 cggcaactct tggacgcatt acgcgctgag aaccgtgttc tgagcgaaag cctgcaagag
     2281 cttccggaag aagcagccag cccggagggc ttctcccctg cgcctcggtc ttttgcgtgg
     2341 ggtgacgtgg cgatcgtgac cgcggtgttg gcactaactt cggtcgttgc tttttggatc
     2401 agtgagctga gcattccgca agcgctgcag tggttaaatc ccttcagcgt aagcggcggc
     2461 acgaacctgg tcttcaactt ttcttattac tttgcaaacg gaggtactgc catgttggat
     2521 gaatatgccg ctgtcattgg gaagatcttt ttggtgttgc tggtgggcgg gagcgcgcta
     2581 ctgctgggac gccgatggcg gctgcgccag cctggcctgc gcctgatgat ggtgctgctc
     2641 gcattgtcat tgcctggctt tgccctggag cggcgccaca gtgagatcgt caccgtacga
     2701 gccaatgaga ctgtagacga caccctgttg gcctccggca atatagtgcg ggtggatggc
     2761 gtggtcaatg gggacttgct ggctttcggc gggacggtgg aagtgcgcgg caccgtcaag
     2821 ggcgacctgg tgagctttgc caagagaatc gtagtgaccg gaaccgtgga aggcaacatc
     2881 tacaacgctt cgaattcgct cgaactggac gggcaattga gccacagcat ttacgcgttg
     2941 gtgcaatcct tgcgtgtggg tgaccggggg cacgtgggcg agggtctggt ggctggcgcc
     3001 ggcgacgtca gcctcgaggg cgaggtgact cgcagtgtga ccatgttcgc tggcaatgcc
     3061 gatgtgagcg gcagcgtcgg ccgcgagctg accatggccg gagataacct cacgctgacc
     3121 aacacggccc gcatcggggg gaacctgagc gcgcgcgtgc acgagctgaa gaacgtacac
     3181 atcgccgacg gtgcgacgat tacgggcacg cgtgacatcc agcagcgcgt aagggaaagc
     3241 catttcacgc gccccagatt ctacttcttc caggcggtct ggctcgctgc cgccatgctg
     3301 gtgggatggt tgggcctggt cctgtttccc ggcttcttcc aggccagcac gcacgcggtg
     3361 ggttcaggct ggcgcagcct cgggcttggc tttgcggtcc tcgcgggcgt gcctctcgct
     3421 atgatcgtga cagccatcac gctggttggc cttccagcct cgctgatgtt gctcatggtg
     3481 tatctggctg cgatctacct ggcaaagatt tgggttggag cgtttctggg gcagatgctt
     3541 ctgaagccga ctggggtgac taagagcgat tggctgctgg gactactggt gggcctgctg
     3601 atcctcaccg tcgtcgggta catcccgtat ttgggcgggc tggtccactt cggtgtggtg
     3661 tgcctgggat tgggagcgtt tgcctggcag ctttatcgga tttcacgacc ggcaatagcg
     3721 gcctgagaat ggctcctgcg gttcgtcgag gcgaagcata aagtaattag aacgcgaaat
     3781 gcctgctcct cgggaagccg agtcgttctc attcgtcagg agcggctggg gtcgattccg
     3841 caagaggatc gggtggccaa gtcgagctaa aacaggattc tcggcagccc atcatcaggg
     3901 aagtcaaatc ccctgaaatg ccccgaatta ttcggggttc gatacgatgc taaagatggc
     3961 ggctcggccc cgaagtcaaa gaagacgcct ggcttgcggc gatgatcccc gaaataaccc
     4021 atccgaattc cgcaaaattc cctgtattat tccctgttag cggcggtttg gacgcaaaac
     4081 ggttcgctcg tgactgcgtc cgctgccatg cttcttaggt tgcgacatcg tgctggatgt
     4141 tgtgccgcca ggtgtagttg cacgcaatcc ccaggagaaa agatgccaac aagataccgg
     4201 gttgtcaggg tcaaggacgt ggagccggaa accttcatta ttgtaggaat cgatttcgac
     4261 gaacacacca tcaagagcac atcgaaggcg atgtcggaag cagccctgcg cgcgcacttg
     4321 cgcgaagccg gcgcttccgc ggatgaaacc accacctgga tcgagcaagg ccgagcctat
     4381 cctgaataaa gaataaagct ggaggtggat tgcgatgagc gtcaccgata ttctgctggc
     4441 gccgatgcag ggcgcggaac accgcgaagt cggcggagtg cagcttgata cggtcgcggc
     4501 tggcaacagc cgcgtgaagc gtgtgattta tcccgtgggc ttccgctggt cgaaaaacat
     4561 gaaaccgctg gtgggcacgg accggtgcat gcacgcccat gtcgggttcc tggcgcgcgg
     4621 ccagatccac atggagttcg ccgacggttg cgtgctggaa tttcgcgctc cgcaggttat
     4681 tgccatcgaa cccggtcacg acggctgggt agtgggagac gaacccgcgg tggtaatcga
     4741 gttcgacttt ggcagtgaca ccgtgcgcaa cttggggatg cccgaggaac attcgcacgg
     4801 cgcctgagga aaccggagtt gtcgctgcgg ctacagtaca tcgatgacaa gtagcgtaat
     4861 gtcgtcctgc tgagtcatgg acgcgggctg ccagttgcgg acttcggcga gcagatgctc
     4921 cgacagctcg cttgccgtac acagctgatt gccgcgcaca acttgctcca gtttcaggtc
     4981 tccgaacgat tcgcccgccg cgttttccgg ttccactacg ccatcggtat acattagaac
     5041 gcggtcgccc gtttccagtg ctacgtcgca caccggatat tccgcatcgg gcatgacacc
     5101 gaacagcatt ccgttgctct caatgcgttg cagttctccg gtactggcgc gccagcacag
     5161 aagcggtgga tgtcccgcgg cggagtatcg ggcccggtgg gcctccgtgt cgatccaaag
     5221 ataagcggcc gatacgaact gtccgcgcag ttgccccgag agaatgccgc tcaacctccg
     5281 caacacttct ccgggatcat cggcccaggc ggtcaccgat tgcgccgcaa ccttgatcat
     5341 cgacgcgatg agcgcggcag gtacgccgtg tcccgagacg tcggctacca ggaagccagc
     5401 gcggtgctgg tccaccgcga ggaattcgta gaaatccccg gctacggcgg tcatcggcaa
     5461 ataatttgcg gccacgcgga gacttctgag ttccggaaca ctcgttggca ggatcgagaa
     5521 ctgcaactgg cgggcaatct ccagttcgtt ctcgatcgaa agcagtcgtc gctcgttccc
     5581 gaagaccatc tgcagggcga catagccaaa ggaaaacagc agcacagcga aggccaacga
     5641 atcccagacg cgagccgtct catggccgaa gggacgcgac aggttgacaa aaactgcctc
     5701 agcgacgaac acgatgctgc ccacggccag cgcaccgcga ccgggcaaca ccagatactt
     5761 ccgcgacaaa ctcggcacca gaataaccgt cagcaggaca agcaggtcaa caaccgcaag
     5821 gaggttgttc cagggcatca gcttatcggg cgaaccggcg aagacgaagt aagcgaaacc
     5881 agggacgccg atggcgaggc ccgcgaacga aagcgcgcca aggatatgcc tgactttgcc
     5941 aaggctcagc tcccgccagg ccatcgtagc aatcaccacc atcagatagg tgaagccagt
     6001 cctcacatag ggagccgcgg tttgcagcca gcccggcaaa gcggcgacga acgccggggt
     6061 accactcagt ccgagaattc cataagcagc gctccagatt cccatccaga caattgcgct
     6121 cacgccagtg cggcggcgga tggcagccgc cgccacggcc gctaatccga tgactatgaa
     6181 ggtcgtgccg aagcagattt cgatcagctg tccccgcagc atggccagaa cttgagcttc
     6241 aggactcatt gcggacttcc cgtcgattca ggaacgacat tcttcttact accttctgga
     6301 atcgctggct ctggcaggtg catcgattca tcctatccct tgcgcgtcag caggcccatg
     6361 atcggcatcg ccatcatgac gacgaccgtc attccaagca tgaatgtctg tgatgtttgc
     6421 gtccagactg tttgagaagc gaccgcggcg tcatggggac ggcgggtcac cgaaaggctt
     6481 cgacgatcga tgagccactc tgcgcttcaa cggagcggcg agcgacgctg agagaatccc
     6541 accgcgcccg caaactcagc tatctcaatt cacgaaagaa aatcgcgacg acaaccgtgc
     6601 cagcttgagg accttgtaga tatcgacgtt gacgccgcgc aacgtgacct tgaccccctt
     6661 ctcgtcggcc acgccggcga aatctcccaa cgcccgcagc ccgctggaat cgatccgacg
     6721 caccgcggag aaatccagaa acgcatcgcc gccggcgctg tcgagtttct tcccggcctc
     6781 gtgtaaagcc agggccgcac tatttccatc aacttcgacc cacacgtcaa tcattgtcat
     6841 accagcacct tgtcctcacg tctctgctgt gatgcttcct taattcttgc cggcccgcga
     6901 ccgttgtacg cgctccagaa cagttccacc aattcgctca acagcggcat gcggggattg
     6961 gagcgcccgg agggatcgtc aaacgcattc ttgaccaatt ccggcatcgc ccgctcgaaa
     7021 tcttccttcg agatcccgag ttcggcaatc gaccgcggaa tcgccaactg gtcgagtaac
     7081 tgttcgactg cgatcaccag gttctgcact ttttcttcaa ccgtattgcc gcccaggcca
     7141 agcaactccg ccatcatcgc gtattttttg tgcgcgatgt atcccctctg attcggtgac
     7201 ggcatgaact tggttggtac tcccgcgttg taggcaatga cgtgcggtag catgagcgcg
     7261 ttggcccgtc catgcgcgat atggaaacgc gcgccgagag catgagccag agcgtgattg
     7321 acacccaccg aggcattgga aaacgcaatc gccgcaatgc aggccgcgtt gtgcatcatg
     7381 gttcgcgcct cttcatcgtt gggctgtgca taggaaatgg gcaaatactt aaacgccatc
     7441 cggatcgcct ggaatgcgtt ggagtcggtg tagggtgaag cataaatcga aacaccggct
     7501 tccagtgcat gggtcaaaca atcgatgccg gtgtcagcgg tcaggccttt cggcattgac
     7561 atgacgaatt gcggatcgac gatagccact tccggagtga gcgagtaatc ggcgagcgtc
     7621 actttgcgtc cgtgctctct gtcggtcaga acggcaaacg gagtgacttc gcttcccgtt
     7681 ccgctggtgg tggagatggc caccaacctc gagtggttga tcttctcggt ggggtactgc
     7741 accacgcgct tgcggagatc caggaaaggc gcggccagtt cctccaggtc agcctccggc
     7801 gattcatact tgagtttcat gatcttggct gcgtcgatca cggaaccgcc acccagcgcg
     7861 atgatctggt cggcgcggta gaagttgagt ccctccactc cgtgcataat cactttgact
     7921 tcaggctctg cgtcaggaat caccgagacg tgcaccaggg tttctggtgg aaggttgcgc
     7981 cggacagtat ctacatggcc gatttgctcc atggccggat tagtaataat gacggttgag
     8041 cgcgacttga atttccgcaa attttcaacc gcattcatat tgaagtaaat ctcattcgga
     8101 acacggaacc acatatgcgc ttgtgtcctc cgcgccactc gtttgatgtt gaggtagtga
     8161 aacacgttga tgttgtcggt ggtgctgttg ccgccaccgg taccgcagcc gaatgaaaag
     8221 gtcggcacca tgtcgttgta aacgccaccc aacgctccaa tcgatcccgg agaattcaca
     8281 ataatgcggc ccgcgttgat caagtcgccg aatttccgga tgacctcatc attgcgcgag
     8341 aacacgactg cggtgtggcc catgccgccg gcataattta cgtcgataca caccttgagc
     8401 gcttcatcga gggatttcgc gcgatagacc gcgagcaccg gaaacagttt ctccaccgac
     8461 agcggatatc cgcgacctac gcccagaatc ggcgcgatca gcagcttggt ttttggcttg
     8521 accttaaggc cgatcatgcg agcgatgctc gtagctttct gtccgactgc catcgactgc
     8581 atgaacccgg tcttgggatc gatcaccgtg cggcccaaca actccgtctc tttctcagtg
     8641 cagatgtgcg aacccagctc ggcaaatttt tccagaacga tgtcgtaaat ctcgtcgtcg
     8701 atcaccaccg tctgctctga cgcgcagatg gttccgttat cgaacgtctt cgaggtgatg
     8761 atgtcgacca cggccatatc caggtccgcc gacttctcga gataaaccgg cgtgttgccc
     8821 ggccctaccc ccaatgctgg cttgcccgaa ctgtatgcgg ctttcaccat tcctggtccg
     8881 ccggtggcat cgatcaggcc aacgtctttg tgatgcatca tgtagtcgtt gtcctgcagg
     8941 ttgtgcgatt ccaggcaagt aaagacgcct tcgggcgcgc cgtgctttac cgccgtctcg
     9001 tacatgatcc gcaccgcctc gtagcaacag tgaccggcgg cggggtgcgg actgaaaatg
     9061 accgcgttcc gcgtcttgat cgccatgatg cacttgaaga gaacggtgga agtgggattc
     9121 gtaatcggca gtagcgagaa gatgactcct atcggctccg ccatctccac tagattgcgc
     9181 tccgggtact cggcaatctg gccaaccgta cgcttgtctt ttacgtagtt atagacaaac
     9241 tccgtggcca ccatgttctt gatggctttg tcttccataa ccccgagctt ggtttcctca
     9301 atggccaagc gggcgagata ctgagcctgc tcgaggcccg ccaggaccat cggtttcaca
     9361 atgcgatcga catcttcctg ggtgtattgc gtaaaaacgg cagcggcggt cttggcctga
     9421 cgcaccattc cttccaggta ttcaatccgt tcatcggtga tgacggttgc ggtcttgtcg
     9481 ctcatagttc catccttctt tccaattcct tcggccccgg tgtctcgaac tggctgacaa
     9541 caaatgcgtg tgcgctctgt gccggcgaac aatcctcagg ctctcagcgg cgtggatccg
     9601 atagcgaagc ctgatcgtgg ttcgcacgcg cgaaaaaata cagctgcgga agcggaagca
     9661 aacgcggttt cagaccgtcc gtgtcctggc ttctcgtgag cctacgcctg ccctggggca
     9721 ccaagttcag tatcctcggc tgccaaccca tctgactgtg aggggcgtca cttcaaggtg
     9781 tgagacttgg acaaaacccg gccgaaatcg gccttcacgg acgcagtcgc ggaaacccgc
     9841 caacgggtac ctttatgccg cagccgcctt gaccctggct tccgtggcca cgggttcagc
     9901 tgtggcacgg cggatttttg gtccggttat ttccgactcc gcccgcagcc aatcctcgag
     9961 gtcgtgaccg tgttcacagc cccgttcctc gtaaaagcga tacgcctgtg cctgaattaa
    10021 ctcgtgaaaa tcctcttccc aatactcgaa ctcgcgcctg gccccaacgg ctcgcctggc
    10081 gtgctccgcg gtccgcagtt ggacgcggcg aatcttgccg gaaattgtct tgggcagctc
    10141 cgcgaattca atccggcgta tgcgtttgta cgcccccaga cgcttgcgaa tgaacgcaaa
    10201 aatctcatgc gccactgccg cggatggaaa aacaccggga ttcagcgtga tgtacgcctt
    10261 gggaacgttc cagcggattg cgtccggcgc cggtaccacg gcggcttccg tgatcgcagg
    10321 atgctcaatc agcgcgctct ccaactcgaa agggctgatg cgataatctg aggacttgaa
    10381 cacgtcgtca tcgcgcccga cgtaggtata gtatccgtcg gcgtcgcggc tggcgatgtc
    10441 tccggtgtga aagcagccac cctccatgac cttcacggtc cgctccggat cgtctgcgta
    10501 gccagccatc aagctgatgg gcttgggatt cagccgcatg cagatttcac catcggcagc
    10561 ttcgtttcca tcggcatcca gcaaaacgat ttcgtgtccg ggcgaagcct tccctgccgc
    10621 gccgggcttc accgtctgtc cgggaaagtt gcctatgacg agcacggtct cggtctgtcc
    10681 gaaaccgtcg cgaatggtca cgccccacga ctgccggact ttttcgatga tttccggatt
    10741 gagtggttct ccggcgccca ccagttccct gcactgaacg ggatacgcgg ccaggtcctc
    10801 gagcaccagc aaacgccata cggtcggggg tgcacacagc gtagtcacgt ggtagcggga
    10861 aatcgtttcg agggtcttct tggcgtcgaa tcgtgcatag ttatgcacga ggatggttgc
    10921 gccggcattc cacggtgcga agaaactact ccatgcatgc ttgccccatc ccggagagct
    10981 gatgttgagg tggatgtcgt cggggcgcac gccaatccaa tacatggtga caaggtggcc
    11041 gacgggatag ctctgatggg tgtgcattac cagtttcggc tcggctgtgg tacccgaagt
    11101 gaagtacagc agcgctggat cgatcggctt cgtcgctgtc tcaggtgcgt aagtgtcgtc
    11161 ttcccgatac gcgtcccaat atgagatcca accattgacg ggcttgtcca caacgatgcg
    11221 ggaatactcg cctggcagtt ccgagaagct cccggtaaag gcggcattgg cgatcacgtg
    11281 gcgcgcctgt ccccggacaa gccggtcctt gaggtcgtcg ggggtcagca aagtcgctga
    11341 gggaataacc accgctccca atttcagggc cgccagcatg acttcccaca tcgccaccac
    11401 gttgggcagc atcacgatga tgcgatctcc gcgacgaaca ccaattctgc gaagaaagtt
    11461 ggctacctgg ttggagcgac gcgacatctg gtcgtagctc agtttcaact ccgagccatt
    11521 ctcctccaca atccacaagg ccgttttctg gttgccgcga gcaaaaccgt cgaaatagtc
    11581 cagcgcccag ttgaactgcg taagctgggg ccagcggaac ttctcataaa ccagttcgta
    11641 gtcggtgcga tgctgcagga gaaaatcgcg tgcactcagg aaggcgttca ggtctggatt
    11701 cgagggcgaa gggtccattc tggcaaactc ctattttccg cgggttccat gatgttgagt
    11761 atgggcgcca gagtccgctc atcagcactg cttcttgccc gccgcaggtg cccgtcccgc
    11821 aggccaacgc ccaccagcgg caccaatagc agtatcaccc gctggcaaac accctgactg
    11881 tgaggagcgt caccacaaga tgtgagagtt ggccaaacag gctcggttcc cactatccac
    11941 cagtcgcaga ttcagttggt ctttttaaga ggcgaggaag cgagaagctt ttcgagcttc
    12001 tgcagctgtt cttgcgtact gtcgcgcatc cactgacttg gactgggctg cagcgcgaac
    12061 gccgcgtcca ggctctc
//
LOCUS       DASRKK010000804         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1262898, whole genome shotgun sequence.
ACCESSION   DASRKK010000804 DASRKK010000000
VERSION     DASRKK010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262898"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="VFC15_19270"
     CDS             <1..1861
                     /locus_tag="VFC15_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12355.1"
                     /translation="QSYHDPAKALAQWSANNEKGLGYQREATTFLALLTLFAIALYLL
                     GQALGMGRTSAAFVLVVFACGLVVAGVGRGLFISLAERAIVLRPASAECRLPGSISDD
                     NLVELAAEHFARGWVLYESSSDDPVESAKAAKEFGCAVQIRPTFAGANLYFARATNRA
                     NTPQLNEGGFVSLISKDALDKVSQAEQEARDLLTQQGFAPPIDLVGDSGFDTYADGLV
                     KGDRKIVDAGRQATLAALNLDKNSLVSRFNLGLAQLAEGREKEAMETYRQTVALGDPG
                     KDPFVTNDARVIGGAITDLDVFRQYCGGLNDAVYCRQFESTDLPRLKSEFVAAAWPSA
                     KGRTLANSGIKLTDLHLQGSAAGLGWSGHVENLPESPAEKPQDTLAVLWYAYSPDWKA
                     WRVLPAISERVKPAFYAHGDTHLFYSVLQASDARICLQSGTYRAEFYVDGELAGLQEI
                     ILKDENLHPEMFPDLDVAICRPPSWRRWQPHDPDAVWTRGFTEDGNNRGVFVFSFFDP
                     QQDGEAGTEAKALRRAQNILHNEGLAPKPGAARPLNDCTGLRGHPGEAMAAFGDGGGA
                     SIARAWTTKEGLVNVVAVVDKHLDEAGLVTEAPSQSQAHQDCEILLSATTVHK"
BASE COUNT          418 a          598 c          644 g          414 t
ORIGIN      
        1 ccagagctac catgacccgg cgaaggcctt ggctcagtgg agcgctaata atgagaaggg
       61 cctgggctat caaagagagg cgaccacctt tctggcgctg ttgaccttgt ttgccatcgc
      121 gttgtacctg ctgggacagg ctctgggcat gggccggacc agcgccgcct ttgtcctggt
      181 tgtattcgct tgtggcctgg tggttgccgg cgtcgggcgc ggcttgttca ttagtcttgc
      241 agaaagagcg attgtgctaa gaccggccag tgccgagtgc cgcctccccg gctccatttc
      301 cgacgataac ctggtcgagt tggctgccga acattttgcg cgcggctggg tgctctatga
      361 aagctcgtcg gatgacccag tcgaatcggc caaggcggcg aaagaatttg gctgcgccgt
      421 ccaaattcgc cctacctttg ctggggcaaa cttgtacttc gcgcgcgcca ccaatcgcgc
      481 caatacgccg caactgaacg aaggcggatt tgtcagcctc atctcgaaag atgccttgga
      541 caaggtttcc caggcggaac aggaggccag agacctgctt acgcagcagg gcttcgcgcc
      601 cccaatagat ctggtgggag attcaggatt cgacacgtat gcggatggac tggtcaaggg
      661 cgatcgcaag atcgtagacg ccggcagaca agcgaccctg gcagccctta accttgacaa
      721 gaacagtctg gtgtcccgct tcaaccttgg gctggcgcag cttgcagaag gacgcgagaa
      781 ggaggccatg gaaacctacc ggcaaacggt tgcgctgggc gatccgggca aagatccatt
      841 tgtgaccaac gatgcgcgag tgatcggcgg agccattacc gatcttgacg tgtttcggca
      901 atactgcggg gggttgaacg acgcggtgta ctgcagacaa ttcgagagca cggacctgcc
      961 gcgattgaag tcggagtttg tggcggcggc ttggccgtct gcgaaaggac ggacgcttgc
     1021 caactcgggc atcaagctga cggaccttca tctgcaggga tcggccgccg gccttggctg
     1081 gagtggccac gtggaaaacc tcccggagag ccctgccgaa aaaccgcagg atactctcgc
     1141 cgtcctctgg tatgcctata gcccggactg gaaggcatgg cgagtgctgc ccgcgatctc
     1201 agagcgggtg aaaccggcct tttacgcgca cggcgatact cacctcttct attccgtgct
     1261 gcaagccagc gacgcgagaa tctgcctcca gagcggcacc tatcgcgccg aattttatgt
     1321 cgatggcgag cttgccggct tgcaagaaat catcttgaag gacgagaatc tccatcctga
     1381 gatgttcccc gacctggatg tggcgatttg ccgtccgccg tcgtggcggc gctggcagcc
     1441 gcacgatccg gatgcggtgt ggacccgtgg cttcaccgaa gacggcaaca atcgtggcgt
     1501 ctttgtattt tccttcttcg atccccagca ggacggagag gcggggaccg aggcaaaggc
     1561 tttgcggcgg gcgcagaaca tcctgcacaa cgaaggcctg gcgccgaagc caggcgcggc
     1621 gcgtcccctc aacgattgca ccggccttcg cggtcatccc ggcgaggcga tggctgcatt
     1681 cggcgatggc ggaggcgcct ccattgccag ggcgtggacc acaaaggaag ggctggtgaa
     1741 cgtggtagca gtggtcgaca aacatctgga cgaggccggg ttggtaacgg aggccccgtc
     1801 gcaatcccag gcgcatcagg attgcgagat cctgctctcg gccaccactg tgcataaatg
     1861 atgcgggcac attgcctttg gattggcgag gcgggacgct tccttctttt cattccaccc
     1921 acataagttt gtggacgaac ttgtgcgggc gaacccgacg aaacatcgga ctgctcgact
     1981 gccaaaaatc agggcggctc tatgtcaata gaaccgccct gagccttcat ttcaccggtc
     2041 taaatccact gcagcctaag cggacttttt cttc
//
LOCUS       DASRKK010000805         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1165582, whole genome shotgun sequence.
ACCESSION   DASRKK010000805 DASRKK010000000
VERSION     DASRKK010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165582"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFC15_19275"
     CDS             complement(<1..578)
                     /locus_tag="VFC15_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HZM12356.1"
                     /translation="MKANQGAAGVDGQSIQEFEAQLAGNLYKLWNRLSSGSYLPPPVR
                     RLDIPKANGGTRPLGIPTVADRIAQEVVGRYLEPLLEPLFHADSYGYRPGRSAIDAVR
                     TARQRCWRYDWIVDLDIKGFFDSIDRELLLRAVRKHTQCQWVLLYIERWLKAPTMLEG
                     GTLVPRDRGTPQGGVVSPILANLFLHYVFDVW"
     gene            755..2401
                     /locus_tag="VFC15_19280"
     CDS             755..2401
                     /locus_tag="VFC15_19280"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM12357.1"
                     /translation="MFVRTQTNGSRTYLLIVDNVRVDGKVKQRVLHRLGRLDELLASG
                     QLDVLIQSLSRFSEKLAVLGAHAQGDSIVTHSARIGPALIFDRLWQSCSIDQVLTAML
                     EGRRFEFSVERAIFLTVLHRLFAPGSDRAAEKWKDGYAIQDVGDLDLHQLYRAMAWLG
                     EVLPKAQQDGATPFAPRTNKDLIEEALFARRRDLFSDLDIVFFDTTSIYFEGAGGESI
                     GQRGHSKDHRPDLKQMVVGMVLERNGNPVCSELWPGNTADVKSLVPIVERLKSRFGIR
                     SVCIVADRGMISAETLAEVEKREWKYILGVRMRSSTEAKAVVARAGRYAEVHPQSDDR
                     NDPSPLKVKEVWVEDTRRYVVCVNEDQATKDRHDREAVVAALREALGHGDKSLVGNKG
                     YRKYLRAGGQQFAVDEDKIKEEARYDGKWVLTTNTDLPAREVALKYKQLWMVEDVFRS
                     MKSLLDTRPIFHKCDETIRGHVFCSFLALLLRKELEDRLARKEWKLEWGDVLRDLDNL
                     VEMEVAISGKGYVFRGQTSGVAGKVFQACGVALPPVLRAC"
     gene            complement(2508..>2777)
                     /locus_tag="VFC15_19285"
     CDS             complement(2508..>2777)
                     /locus_tag="VFC15_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HZM12358.1"
                     /translation="VVNQEKSRLTTVEEGFAFLGFEFRKPPRRLLYMWPREKACQHIR
                     DRVREVVRSFPSSASIGEVIRKLNPVLNGWCTYFRVGNSNRTFHS"
BASE COUNT          581 a          824 c          838 g          534 t
ORIGIN      
        1 atccagacat cgaacacgta atgaaggaac aggttggcca gaatagggct gacaacccca
       61 ccttgcggcg tcccccgatc cctcggcaca agcgtaccgc cctccaacat cgtgggcgcc
      121 ttcagccagc gctcgatgta gagcaacacc cactggcact gcgtgtgctt gcgcaccgcg
      181 cggagcagga gttccctatc gatgctgtcg aagaaacctt tgatgtcgag atcgacgatc
      241 cagtcgtaac gccagcatcg ctggcgcgcc gtccggacgg catcgatcgc cgagcgccca
      301 ggccgatacc cataggagtc cgcatgaaac aacggttcca ataagggctc caggtatcgc
      361 ccaacaacct cttgcgcaat acggtccgcc accgtcggaa tgcccaacgg ccgcgtcccg
      421 ccattcgcct tcggaatgtc cagccggcgc accggtggtg gcaggtaact gcccgacgac
      481 agccgattcc agagcttata cagattccca gcaagctggg cttcaaactc ctggatcgac
      541 tgcccatcta ctccagccgc tccctggttg gccttcacac tcttgactca tcttcgacag
      601 tttgcagccg aagatccctg taactcattg attctgcttg caggggcggt ctccgatgcg
      661 aaaatgtagt ggcacgtttt cttcaactat gctgagattt gctattgtat tgtggttaat
      721 agtgtgggaa taatggtgac ataggagcgc cgatttgttc gttcgcacgc agaccaacgg
      781 ttcccgaacc tacttgctca tcgtcgacaa cgtgcgggtc gacggcaagg tcaagcagcg
      841 tgttttgcac cgtctcggcc gcctggatga gttgctggcc agcggccaac tcgacgtact
      901 gattcagtcc ctcagccggt tctcggaaaa actcgccgtg ctcggcgccc atgctcaagg
      961 cgacagcatt gtcactcaca gtgccaggat cgggccggcg ctgatcttcg accggctctg
     1021 gcagtcttgc tcgatcgacc aagttctgac ggccatgctc gaaggccggc gcttcgagtt
     1081 ttccgtcgag cgggccatct tcctcaccgt gctgcatcgc ctcttcgcac ccggcagcga
     1141 ccgtgccgcc gagaagtgga aggacggcta cgctatccaa gacgtcggcg atttggactt
     1201 gcaccaactc taccgggcca tggcctggct gggcgaggtt cttccgaaag cccagcagga
     1261 cggggccacg cctttcgctc cccgcaccaa caaggacctc atcgaggaag cgttgttcgc
     1321 gcggcgccgg gacctgttct ccgatttgga catcgtgttt ttcgacacca catcgatcta
     1381 cttcgagggc gcaggaggcg agagcattgg ccagcgcggg cactcgaaag atcatcgtcc
     1441 cgacctgaag cagatggtgg tcggcatggt gctggagcgg aacggcaatc cggtctgtag
     1501 cgaactctgg ccgggcaaca cggccgacgt gaagagcctg gttcccatcg tagaacggtt
     1561 gaagagccgg ttcggaatca gatcggtgtg catcgtggcc gaccggggga tgatcagcgc
     1621 cgagaccctg gcggaagtgg agaaacggga gtggaagtac atcctgggcg tgcgcatgcg
     1681 aagttcgacg gaggccaagg cagtggtggc gcgcgccggt cgctatgcgg aagtgcatcc
     1741 ccagagcgac gaccgcaacg atccctcgcc gctgaaggtg aaagaagtct gggtcgaaga
     1801 tacgcgccgg tacgtggtgt gcgtgaacga ggatcaagcc accaaggacc gccacgaccg
     1861 cgaggccgtg gtggccgcgc tacgcgaagc gttaggccac ggagacaagt cgctggtagg
     1921 caacaagggg tatcgcaaat acctgcgcgc gggaggccaa cagttcgccg tggacgagga
     1981 caagatcaag gaggaagcgc gctacgacgg caagtgggtg ctgacgacga acacggatct
     2041 gccggcgcgc gaggtggcgc tgaagtacaa gcaactgtgg atggtcgagg atgttttccg
     2101 ctcgatgaaa tcgctgctgg atacccggcc gatctttcac aagtgcgacg aaacgattcg
     2161 cgggcacgtg ttttgctcgt tcctggcgct gctgttgcgc aaggaactgg aagaccgtct
     2221 ggcgcgtaag gagtggaagc tggaatgggg cgacgtcctc cgggatctgg acaacctggt
     2281 tgagatggaa gttgcgatca gcggcaaggg ttacgtcttt cgcggccaga cttcgggagt
     2341 ggcgggcaag gtttttcaag cctgcggcgt cgccctgcca ccagtcctgc gcgcatgctg
     2401 aattttcagc cggttcggct caggtggaga gaaagcgtgt cactacgccg ttttcaaaat
     2461 cgcaacctat tggaaatgga ccacttccgc cggcatggtg tagaagatca gctatggaat
     2521 gtccggttac tgttgccgac ccggaaatag gtgcaccacc cattcaagac cggattcaac
     2581 ttccgaatca cttcgccgat cgacgcgcta ctgggaaacg atcggaccac ctcgcgcacc
     2641 cgatcccgga tatgttggca cgccttctcg cgcggccaca tgtacaacaa ccgtctcggg
     2701 ggtttccgga actcaaaacc aagaaacgcg aagccctcct ccaccgtggt cagacgactc
     2761 ttctcctggt ttaccac
//
LOCUS       DASRKK010000806         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_578897, whole genome shotgun sequence.
ACCESSION   DASRKK010000806 DASRKK010000000
VERSION     DASRKK010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578897"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..706
                     /gene="istB"
                     /locus_tag="VFC15_19290"
                     /pseudo
     CDS             1..706
                     /gene="istB"
                     /locus_tag="VFC15_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544395.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(862..2517)
                     /locus_tag="VFC15_19295"
     CDS             complement(862..2517)
                     /locus_tag="VFC15_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM12359.1"
                     /translation="MGAIAISSFVLVGGGAPLAAQTSEKPNIVMLMTDDTGWNDFGAY
                     SLGGANLGHPTPNIDKMAKEGAVFTSWYGQASCTAGRASFITGRIPIRSALSIVVAPG
                     DENYLKPETPTIAEFFKKNGYTTYFSGKWHLGDKPVAYPTNHGFDEMKEFAAYYAGVY
                     SYPDTSKNFHPWFPSYNPEFEKMFDSVVNLYEWEGVAGQPAKQVAKITYDYMATMDER
                     QADYAVDYIKTHAKSGKPFFMDVNWIKMHNPTNAAPDFRGKSHLGDYSDSLMEMDADI
                     GKVLDAIHQYAPNTIVILTADNGAWLDAYPDAGTTPFRGEKGSAFEGGWRVPAIMTWP
                     GHIPAGVQYGEMMSHIDCWSTLASMAGITRPAHGEMKDNNGKPIYFDSIDNSAYILGK
                     AKHSTRTSWVYITGEYLNAVRADISGDPNNPDLNIAWKFLWTAKDSWLGPSQSLGSVP
                     ALYNLTMDPFERYDMIFNGAEGAMNGLKTSPGRYAGEDNGWAASLMGIVLADFDKSIM
                     QYPSIQRAPGGASNDMIPNLQNPKNPLPLLDPNNLPKTGASSD"
     gene            complement(3048..>3265)
                     /locus_tag="VFC15_19300"
     CDS             complement(3048..>3265)
                     /locus_tag="VFC15_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12360.1"
                     /translation="GTVSPMSWERDKTGPLALNPAAESAESKASAAARVGGFFGSISI
                     RSPESGSYELRMYSVGFHPASLSTAES"
BASE COUNT          670 a          912 c          961 g          722 t
ORIGIN      
        1 ggcatggtcg aggccctgaa aacgcaggag caagatcgag cgatcaacga tctgagtttt
       61 ctcgaacgac tcgggctgct ggtcgatcaa caatggactt ggcccgagaa ccaggcgctg
      121 gagcgaagac tgaaagcggc caagctgaga ggaccagcct gcggtggagg acatcgacta
      181 tcgtgcggcg cggggtttgg aaaagacggt aatgcgtgcc cctggcaaaa gactcggcct
      241 gggtccgcaa ccacgagaac attttcgtgt tgggttcgac cggcgtgggc aagagcttta
      301 tcgcgtcggc cttggcccag aaagcttgtc gcgacgggta ctcggctctc tatttgcgag
      361 ctgccgcgtt gctacgtgaa ttggccctgg cccgagccga agcttgcgtc acctcctggc
      421 acgactggat cgcatcgacg ttctggtgat tgatgactgg gccatggccc cgctgaatga
      481 aagcgagcgc cgcgaagtct gggagatctg cgaggatcgt taccagactc gctccaccat
      541 cctcacttcc cagttgccgg tttcgcgctg gcacgagcag attggcgatc ccaccatcgc
      601 tgacggcatc ctcgaccgcc tggttcacaa tgcccaccgc atcgagatgc gcggagagtc
      661 catgcggaag aaacgcaatc cgccgcagga cgagaagaaa gaatgaacga aagtcaacat
      721 ctttgagtgc gaaatggcga tttcacgcca gtaaattcga tgcgcacgtc agttccccat
      781 tgctcacgaa ccgaatcgtc ccgagatgga aaaagggcat gcagtgtcgc acgccctttt
      841 gctagaagta aaagccgcga tttagtcgct ggaagcgcca gtcttcggca agttgttcgg
      901 atccagcagc ggtagcgggt tcttaggatt ctgcaagttc ggaatcatgt cgttcgatgc
      961 gccaccgggt gcacgctgga tgctcgggta ctgcatgatc gacttgtcaa agtcggccag
     1021 cacgatgccc atgagcgaag ccgcccagcc gttgtcttcg ccggcatagc ggcccggcga
     1081 ggtcttcagg ccgttcattg cgccctcagc tccgttgaag atcatgtcgt atctctcaaa
     1141 cggatccatg gtgaggttgt acagcgcggg aacagagcca aggctctgac tcggccccag
     1201 ccaggagtcc tttgcagtcc agaggaactt ccaggcaatg ttcaggtcgg gattattggg
     1261 atcaccggag atatcggcgc ggacagcatt gagatactcg cccgtgatgt aaacccagga
     1321 agtgcgcgtg gagtgctttg ccttacccag gatgtacgcg ctgttgtcga tgctgtcgaa
     1381 gtatatgggc ttgccgttgt tgtctttcat ttcgccatgc gccggacggg tgatgcctgc
     1441 catggatgcc aaagttgacc agcaatcgat gtgcgacatc atttcgccgt attgcacgcc
     1501 tgccggaatg tgccccggcc aggtcatgat ggcgggcacg cgccagccgc cctcaaacgc
     1561 agagcctttc tcgccgcgga acggagtggt tcccgcatcg ggataagcat cgagccatgc
     1621 gccgttgtcg gcggtcagga tgacgatggt attgggagcg tactggtgga tcgcgtccag
     1681 caccttgccg atgtcagcat ccatctccat aagtgaatcg gaatagtcgc cgagatgcga
     1741 cttgccgcgg aaatcgggcg ccgcattggt cgggttgtgc atcttgatcc agttcacgtc
     1801 catgaagaac ggcttgccgc tcttggcgtg agtcttgatg tagtcaaccg cataatcggc
     1861 ctggcgttcg tccatggtcg ccatgtagtc gtaagtgatt ttggcaacct gtttggcagg
     1921 ctgaccagcc accccttccc attcgtacag gttcaccacc gagtcaaaca tcttttcaaa
     1981 ttccgggttg tacgacggga accaaggatg gaaattcttg gacgtgtccg gataagaata
     2041 gacgccggcg tagtaggcgg cgaattcttt catctcgtcg aaaccatggt tggttgggta
     2101 cgccaccggc ttgtcgccca ggtgccattt gccggagaaa tacgtggtgt agccgttctt
     2161 cttgaagaat tcggcgatgg ttggtgtctc aggtttgagg tagttttcgt cgccgggagc
     2221 aaccacgatc gacagcgccg agcggatggg aatgcgtccg gtaatgaagg acgcgcggcc
     2281 cgcggtgcaa ctcgcttgtc cgtaccaact ggtgaagacg gcgccttctt tggccatctt
     2341 gtcgatgttg ggtgtgggat ggccgaggtt agcgcctccg agtgaatagg caccgaaatc
     2401 gttccatccg gtgtcgtcag tcatcagcat gacgatgttg ggtttctctg aggtttgcgc
     2461 cgcgagggga gcgccaccac ctaccagcac gaagctggag atggcaatcg cgcccatcgt
     2521 aaattttcga actcctgcac caaaatccat aggtctcctg ttatgcactt ccagttggct
     2581 cccgcgaggg agattatgat cgactgccgc ctcagtgcta tggcggcggt ttgagaactt
     2641 cagctgttac ctgttgttac aggatatact cgcaaccctt cgtcggttta tttctcacag
     2701 ccgactgctg catggaccgg gggaacactg gaggttgggc tttgcgccga tagtcggcgt
     2761 gatcaggcca tctcgcaccc aatccttcca ggacagaatt atcagcgaat cccactcgtc
     2821 tcgctgtcca gaactggctt ccgggaggtt ggcacgccaa ttcaagccca tagggtttta
     2881 aggcagcgct tatgagtggg agcggcccat gatccttctt ctacaacgag gaagcagggc
     2941 aaaggggctg acgccccttt cccctgcacc ccaatccccg ccgaacccgc ctccgctttg
     3001 ctccggacaa tttcgcttga cgattccgaa gtggctgacc ttgcctatca actttccgca
     3061 gtgctgaggg atgcagggtg gaagccgacc gaatacatgc gaagttcata cgagccactt
     3121 tcaggacttc ttatagaaat cgacccgaag aatccgccta cccgagccgc ggcagatgcc
     3181 ttgctctccg cgctctctgc tgcgggattg agcgcaaggg ggccagtctt gtctctttcc
     3241 cacgacatcg gactcaccgt acctc
//
LOCUS       DASRKK010000807         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1165873, whole genome shotgun sequence.
ACCESSION   DASRKK010000807 DASRKK010000000
VERSION     DASRKK010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165873"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFC15_19305"
     CDS             <1..289
                     /locus_tag="VFC15_19305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZM12361.1"
                     /translation="QEETNSTPWLSELVYQAATNLGYQSHFYQFKAGIEDDHLPFLKI
                     GVPAVDIIDLDYGYNNVFHHTVQDTLDKLSPQSLAISGDVVLETIRLMNAR"
     gene            complement(477..959)
                     /locus_tag="VFC15_19310"
     CDS             complement(477..959)
                     /locus_tag="VFC15_19310"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZM12362.1"
                     /translation="MHPKIVQLDGFEVIGIAVRTNNAKEGGPDGVIPKLWQRVMQEHV
                     LDRVPDKADPNLYAAYTDYASDANGDYTLVLGAKVRPETNPPAGMTSKSVPAGRYAVF
                     TSERGPVAKVVVETWMRIWAYYQSSANGERGYRADFEVYDERAADPNNAHVDIYIGVK
                     "
     gene            complement(949..2166)
                     /gene="solA"
                     /locus_tag="VFC15_19315"
     CDS             complement(949..2166)
                     /gene="solA"
                     /locus_tag="VFC15_19315"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZM12363.1"
                     /translation="MARMPKVADVLVVGLGAVGSAALYQSAKLGARVIGIDRFNPPHD
                     QGSSHGDTRITREAIGEGREFVPLVLRSNQIWQELEGATGRSLLTRNGGLVLASPSMP
                     GNHHGSTSFIQDTIAVATEFGIAHEVLSADELRHRYPQFCLRGDEIGYFEPGAGFLRP
                     EACIETQLTLARHLGAQVHTSETVVDCKPLQDSTVEVATGANTYCATKVIVTAGPWIQ
                     KLLGDSYARYFKIYRQVMYWFALASHAEQYTPECFPVFIWIAGNRPRDMMYGFPAVDG
                     PLGGVKIAVEQYNATVDPDAVPRAVSDKEISAMYTEYVAPHFPDVSGKCVRSATCLYT
                     VTPDAKFIVDRFRNYENIVFASACSGHGFKHSAALGEALALQALGRPSRIDLSGFSPW
                     RFARDGWRNSCAP"
     gene            complement(2442..>2810)
                     /locus_tag="VFC15_19320"
     CDS             complement(2442..>2810)
                     /locus_tag="VFC15_19320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM12364.1"
                     /translation="AAYDESVANYREAVLTAFQQVEDGLSGLNALSQATTSQNAAVSD
                     ARRALQIANNRYVGGVTTFLDVITAQSALLTNERLATQLLGQEMVTSVYLVKAVGGGW
                     DASQIQNEQVRPKAIQAIQQ"
BASE COUNT          568 a          819 c          837 g          586 t
ORIGIN      
        1 ccaggaagaa accaactcga caccttggtt gagcgagctc gtttaccagg cggccaccaa
       61 cttgggctac cagtcacact tctaccaatt caaggccggc attgaggatg accatctgcc
      121 tttcttgaag attggcgtac ccgcggtgga catcattgac ttggactacg gctacaacaa
      181 tgtcttccac catacggtgc aggacacgct agacaaactg agtccgcagt cgctggcaat
      241 cagcggcgat gtggtgctgg aaacgattcg gctgatgaat gcgcggtagg cagcgcgatt
      301 cccaagatta ctgaagtgtc atcctgagcg agcgcagcga gtcgaaggac ccctattccc
      361 gcaggatcac cgcgtgcctg gcatagcaaa tttgggattg cttacaagat agggatcctt
      421 ctactcctcg ctgcgctcgt tgctcaggat gacacctcaa actacgacgt gaactcctac
      481 ttcacgccga tgtagatgtc tacgtgcgca ttattcggat cggcggctcg ctcatcgtac
      541 acttcaaagt cagcgcggta gcctcgctcg ccattggcgg acgattggta gtaagcccag
      601 atccgcatcc aggtttcgac aacgactttc gcaaccggcc cgcgctcgct ggtgaacacg
      661 gcgtagcgtc cggcgggaac ggacttggaa gtcattcctg cgggagggtt tgtttcagga
      721 cgcactttcg cgccgagcac cagcgtgtag tcgccgttgg catcgctcgc gtaatcggtg
      781 taagcggcgt agaggttcgg atcagctttg tccggaacgc gatcgaggac atgctcctgc
      841 atcacgcgtt gccacagctt cggaatgacg ccgtcaggtc ccccttcttt ggcattgttg
      901 gtccggactg cgatgccgat gacttcaaac ccgtcgagtt ggacgatctt agggtgcaca
      961 ggagtttcgc caaccgtcgc gcgcgaatcg ccacggactg aatcccgata ggtcaatgcg
     1021 cgagggccgt cccagcgcct gcaacgcgag cgcctcgccc agcgccgcgg aatgcttgaa
     1081 cccgtggccg gagcaggcgg aggcgaagac aatattctca tagttgcgaa accgatcgac
     1141 aatgaatttc gcgtcgggcg tgaccgtgta gagacaggtc gcgctgcgca cgcatttgcc
     1201 ggagacatcg ggaaagtgag gtgcgacgta ctcggtgtac atggcggaga tttccttgtc
     1261 gcttaccgca cgcgggactg catcgggatc gaccgtcgcg ttatattgct cgacggcaat
     1321 cttgaccccg ccgagcggtc catccacggc agggaagccg tacatcatgt cgcgcggccg
     1381 gttgcccgcg atccagatga ataccggaaa gcattctggc gtgtactgct cggcatggct
     1441 ggctagggcg aaccagtaca tcacctgccg gtagatcttg aagtaccttg cgtagctgtc
     1501 gccgagcagc ttctgtatcc acggcccagc ggtgacgatg accttcgtgg cgcaataggt
     1561 attggcgcca gtcgcgactt cgaccgtgct gtcttgcagc ggcttacaat cgacaacggt
     1621 ttccgaggtg tggacttggg cgcccaaatg tcgcgctaac gtgagctgcg tttcgatgca
     1681 agcctcggga cgaagaaatc ccgcgcccgg ttcgaagtag ccgatctcat cgccgcgcaa
     1741 acagaactgc ggatagcgat gccggagttc gtccgcgctc aaaacttcat gcgcgatgcc
     1801 gaactccgta gccacggcga tagtgtcctg gataaacgaa gtcgaaccgt gatgattgcc
     1861 gggcatgctg ggtgaggcga gcaccaatcc cccgtttcgg gtgagcagac tgcggccggt
     1921 cgcaccttcg agttcctgcc agatctgatt ggagcgcaac accaggggca cgaactcacg
     1981 gccctcgcca atggcctcgc gggtaatgcg ggtatcgccg tgcgacgagc cctggtcgtg
     2041 cggcgggttg aagcgatcga ttccgattac acgcgcgccc agcttggcgg actgatacag
     2101 cgccgcgctg ccgacggcgc ccagaccgac caccagcaca tccgcaacct tgggcatacg
     2161 cgccatgata agccatcggg cgagttccca acggagaggc aagagagcac ttggcacttg
     2221 gcatttggtg caaaggctcc ggtgaatttg ctagttgcgc ccggggccgt tattttgacg
     2281 cgagataacg ccgcgcgtaa tgttgcctcg atcaatctga cagatatagc gggctggtgg
     2341 aagtgtgccg cgtgttcgca gaaatcaagg aaagagtcgc aatttgttgg gagttttgcg
     2401 gcttttggtt aactgctatt tgcgctagtt gctagatgct tctactgctg aatcgcttgg
     2461 atcgccttcg gccgcacttg ctcgttctgg atttgtgagg catcccaacc acctccaacg
     2521 gccttcacca gatacaccga agtcaccatc tcctggccga gtagctgcgt cgccaggcgc
     2581 tcgttggtca ataacgccga ctgtgcggtg atgacatcca gaaatgtagt cacgccgccg
     2641 acgtagcggt tgttggcgat ctgcagcgca cgtcgcgcgt cggacacggc agcgttctgg
     2701 ctcgttgtcg cctgcgaaag cgcgttcagc cccgacagtc catcttccac ctgctggaat
     2761 gcggtcagca ccgcctcgcg gtagttggcc acagattcgt cataggcagc
//
LOCUS       DASRKK010000808        23841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1165954, whole genome shotgun sequence.
ACCESSION   DASRKK010000808 DASRKK010000000
VERSION     DASRKK010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 23841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23841
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165954"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..979
                     /locus_tag="VFC15_19325"
     CDS             317..979
                     /locus_tag="VFC15_19325"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZM12365.1"
                     /translation="MANLFEPPRYIAVEGPIRVGKSTLSQVIAETLHAQRISEPDRNP
                     FLKPFYNGEAGAAFQAQFAFLIARYEQLASLDVKRNPNKTVVADYIFEKDKLFACVNL
                     TDAELEVYNRYYELFRQNVPTPDLVIYLQATPEVLKKRLKKKNAPGENAINDDYLEEV
                     IKAYEHFFFHYTSSDLLIVNTSEIDFVEHSQDLQALLRRVSEPIRGTQYFLPLGSDDV
                     KS"
     gene            1109..2476
                     /locus_tag="VFC15_19330"
     CDS             1109..2476
                     /locus_tag="VFC15_19330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM12366.1"
                     /translation="MGVLFQAFYWNCPAAENQDGTWWNFVASKVPELQQAGFTALWLP
                     PACKAANLGGPSMGYDPYDYYDLGDVNQKGRVKTWFGNQAELTTLVDATHTAGMQAYA
                     DLVINHNNGGDAQELNPIDGNTRWTKFNPGSGKFARDWRSFHPSPYETFDEMSFGDMP
                     DMCHRNPGVYENLINLAQFMIETIGFDGFRFDFVKGYGPWMVKSIAELRYLNKADGGF
                     YPFCVGECWDNARTIDDWLTSINTFMDNPVSAFDFPLHYLLKGVCDTYGFSLSTLAQP
                     AGTVVQDTPLRSVTFVDNHDTIRDDGSAILHDKLMAYSFILTHEGYPSVFWMDWYNFG
                     LAQTGTSNGIAALVTAHEHYAGGATQVLFAGDDVYVMQRVGDASHPGLVYVLNNRGDS
                     WNGATVQTQWNNVRFEPVAWGGHDTSRPATKWTQSDGHGDFWAGPRGWAVYAPQLQAT
                     ARTES"
     gene            complement(2473..2745)
                     /locus_tag="VFC15_19335"
     CDS             complement(2473..2745)
                     /locus_tag="VFC15_19335"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZM12367.1"
                     /translation="MARMVNCVKMGREMEGLDEPPFDGPLGQRIYDNVSKLAWEQWQE
                     HMKMLLNEYRLQPWKKEHQEFIVQQMEAYFFGEGAEKPKDFVPPAH"
     gene            complement(3064..3552)
                     /locus_tag="VFC15_19340"
     CDS             complement(3064..3552)
                     /locus_tag="VFC15_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM12368.1"
                     /translation="MTQRQFLLVCVVCFLLCFVAGSAAQTLQIINTDGASTTLTVAQI
                     ASSPHVMVNVKDHDVPAQFEGVPLLTLLASAGVQLGDKMRGPRLTEMLLVEAADGYKV
                     VFALAEIDPAFATREIILADKRDGKPLDPKEGPFRIVAPGDKRPARWVRQVTALRIAV
                     VK"
     gene            3630..4040
                     /locus_tag="VFC15_19345"
     CDS             3630..4040
                     /locus_tag="VFC15_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM12369.1"
                     /translation="MPKLYTPRDAAQVLGISYASLKQWIYQGKLKSVQTLGGHHRIPE
                     SEIDRLLPRVIVSGTPEKRRRTFRRISGRNQLVGRITDVKISGLLAQVTLSVGGQHIT
                     SIITADAARELRLTKGMVAAALIKSTEVMIIIPD"
     gene            4156..4476
                     /locus_tag="VFC15_19350"
     CDS             4156..4476
                     /locus_tag="VFC15_19350"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM12370.1"
                     /translation="MSARFRLHLVGVSLLFLCALSARAQVSASISGRVTDQTGAVVAG
                     ATVTAKSLDTGVSRSTPTDQAGRYELIALSIGQFEVRAARTALRNASAPASFSWWVRT
                     LPSI"
     gene            4473..7367
                     /locus_tag="VFC15_19355"
     CDS             4473..7367
                     /locus_tag="VFC15_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12371.1"
                     /translation="MSLPVGNVSEQVTVEGDASMVNATTQDISGLIGEKEVKELPLNG
                     RSYDLLLTLNPGIVNFTGEKTGGIGVSNSTTGNNFSLSGNRPQQNLFLLNGVEFTGAA
                     ENNMQPGSPSENLMGVEAVREFNVLRDSYGAEYGKRPGAQVTIVTQSGTNQVHGSVYE
                     FLRNNALDAPNYFDQGSAPPFQRNQFGAALGGPIQKNKTFLFANFEGFIQNLHQTSVA
                     FVPDLASRAKAVPSVQPLLNLWPTPSPTDPDFSGIAQVSSSPLQTIREYFGTARLDHN
                     FSANDTFSAVYTIDDGGDVTATTINPYSSDILNLREQVATLEETHVFSSTLLNTARFG
                     FSRAGYFFTGEPTPGTPAATVPGFLVGKQVGAVVVGGSAASNPQAQIGLAGSNNGSNL
                     TMARNLYTYEDRVSWTHGANQFSFGAWFQQFQSNETIALSQYGQATFTSLATFLTGVT
                     STFLYDPAPTEMNWRSLFGALYAQDVIRVSPNFTLTLGFRGEFSTGWNEAHGRAANYT
                     FTNGVISNQPRIAGGAFTTNNAKFLPQPRIGFAWSLYPGKTVVRAGFGMYNELQDALG
                     YRMDQNVPFNPTYSIAAQTVAKLPIDPNAPVPPKALLVPGGVQPNLSMPTLISYTLRV
                     EQELTPNATLTLGYVGSHGYHEIVGLDADTPIPTICPASPCPANYPKSFPAGLAGTPV
                     PAGSYYIPLGTPKANPTLANTWTWFSYGTSYYNALQVDLNHRFSHGFSLRGAYTWSKA
                     LDDGDSVNATTAGNAPGLVSNPYDIKADWGPATYDVRQIGVISAVYDLPFGRGQAYVN
                     SLDGLANGLISGWQVNSIITLQSGFPFTPQLSYNPSNNGDTRNPVRPFANPNFNGNAI
                     LGNPSQWFNPSAFLQPPPNSGFYGNLTRDNLTGPGLATWDFSAIKNTTIHERLSLQFR
                     AEIFNLLNRANFNTPSLIVFTPTGVSGTAGAITSTSTTARQVQFGLKLLW"
     gene            complement(7377..7583)
                     /locus_tag="VFC15_19360"
     CDS             complement(7377..7583)
                     /locus_tag="VFC15_19360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZM12372.1"
                     /translation="MTLSARNQLAGTVEEVQLGGVMAHVVVRVGENLIESVITRRSAE
                     AMALEKGDAVKVVIKSTEVMLQKD"
     gene            complement(7652..10957)
                     /locus_tag="VFC15_19365"
     CDS             complement(7652..10957)
                     /locus_tag="VFC15_19365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM12373.1"
                     /translation="MNVIRNVLIFVVLACLSLPAQQITGSIRGTVTDPMGAIVVGAKV
                     TARQSETGLSRTVTTDRTGNYVVLELPVGHYRLQVTAKGFQEYVQDGISLNVNETASV
                     SPHLVVGSEIAVVQVTADADLIEPGVTAMGKVVGERELEDLPLNGRNFSQLGLLQPGV
                     VPITPGLVEAGGGLRDGQPYSVNGQRPESNNFLIDGADNFNGVDGGYVLKPPVDAISE
                     FLIITHNASAEFGDALGSTTNIVTRSGTNYFHGALWEFLRNDAVDARNYFQTQALPLR
                     QNQFGATIGGPIRKDKIFFFGYYEGFRNLQGVTALTTVPSLQERTGNFAELCPEGFTH
                     GFCNNPANQLFNVLANAPYPYNQLPSINPVSQNLLQYFPAPNVGNNLYSASGEINNDY
                     NQFGIKVDDYLTQHDNLNFRYSFYQSSDVNPLSPQGASVPGFPVGEGQRAQNFVAQET
                     HLFTPNLVGVLRFSFLRNKFLYGQSEVHTTPESLGFQYSSSLPVAEGPPFIQISGYTN
                     IGNPITGPRNTYENLFDYSGSLSWSHGKHNLKFGGGYQYQQINVTQGIATNGFFVFAP
                     FPVTDAFASFLSGTPVVFLQGLGNFTRHIRGNDLNFYAQDTYKVTAKFTLNLGLRYDL
                     PMPDTEVNNLQSMFIPGRQSVVMPDAPRGLLYPGDPGIPAGLIPTDKTAFAPRVGIAY
                     DVFGDGKTLVTSAYGIFYEPYYTGQGGPLQTPISAPPYLATPQVSLPNFADPFNGNPP
                     IPGSFSTPLTNLTLAPNMTLPYSQDWDLNVQRSFGRDFLFEMGYVGTKGTHLPRFIEG
                     NPAVFVPGYVNGQPISNSGNANQRRLYSGCTLNDPPSSCIFSSMGEIASIANSSYNAL
                     EASLQKRFSHGVSFLASYTWSKAIDDASSFNMTGSAAKPVAGENDLAQNPFDLAAERG
                     LSMFDARHRFVFSYVWDLPFWLNGREWYKQALGGWQLSGIVTLMTGTPFTVFDSNDVA
                     AQGSAPEITGFSAQRPDLVGNPNNGPHSVTAWLNASAFVRLDPTLDAGQFGTAGRNIA
                     TGPGYMDWDFGVLKNIKLAESMSLQFRAELFNILNHPNFRLPDSDISSPTFNQILAAR
                     DPRQVQFALKFVF"
     gene            complement(11509..11634)
                     /locus_tag="VFC15_19370"
     CDS             complement(11509..11634)
                     /locus_tag="VFC15_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12374.1"
                     /translation="MIRQIVPQFFTTDIPGTLAYYKDKLGFECLGTWQDPRYTPS"
     gene            12004..12750
                     /locus_tag="VFC15_19375"
     CDS             12004..12750
                     /locus_tag="VFC15_19375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM12375.1"
                     /translation="MFFYRESVDVDYSMEIQRQSGVTLCVNSYGGEIETFAQNIIKAS
                     TIDTLKFLHDESFELRDRFPQDIACMANAHALEENTRDVIDPLISNKEACCISISTSY
                     GAGPDQIFLDSPKAEWLWEYAQDKDVLVHIHPPLVAIGAASMQQYRLNEAVGRPFDTA
                     LSAARMIYSGVFDKYPKLKVLFVHMGGALAPVICRLDWNWELNYKGIQNPPIDKVDKN
                     LRKPSEYFKSNIYVDTMGPSAICLKAMIEM"
     gene            complement(12939..14462)
                     /locus_tag="VFC15_19380"
     CDS             complement(12939..14462)
                     /locus_tag="VFC15_19380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM12376.1"
                     /translation="MTESSNSPFNRREFIQGALAGAALSALPIPSFAAANPDMQTVLA
                     QIPKMQDENVKHLQEWIALPSIAAENRNYPQGPEYMAKLAREAGFTDVEIIPTSGKPG
                     VFGVINAGAPTTVGIYFMYDVKQFDPAEWSSPPLEGKIVDKQGLGKVCMGRGAVNQKG
                     PETAFLSALRAFKEAKVNLPVNLVLACEGEEEIGSPHFPEIVKNPKVMAALQKSMGVF
                     MPSAGQEADGNVEIDLGAKGVVELELVSTGEKWGGGHKHDVHSSLEATVDSPTWHLVQ
                     TLNTLIQPDGHTPAVTGFFDKAKPLTPVQVKMVEAVAARRSEATMKQQLGVEHWAGDK
                     QWLDAMIALESQPTINIEGLVAGYTGPGGKTVLGHRAVAKIDMRLVPDMTASGTLALL
                     KDYLAKKGFGDIEVNMSGGYDPNQTSPDTKLIQAQMATYKKLGQDPLLWPRAAGSWPG
                     YIFTNPPLSLPAGHFGLGHGTGAHAPNEYYLIDSTNPKVAGLDGAVGSFVEYLYALA"
     gene            complement(14565..15737)
                     /locus_tag="VFC15_19385"
     CDS             complement(14565..15737)
                     /locus_tag="VFC15_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM12377.1"
                     /translation="MADPLIDDINVPGGSEVFPSPEQSGPQPLQRAAPERPSSAELEQ
                     HRIQRVQAAALVILATGAVLSLLYVAKLILVVILTSVLLAFVLAPVADALANFRVPRP
                     LGSLIAVFLLVASIGTVSYLSYARAVEFMMQMPEYKIRLKHIVDEIRQQAEQFEKTTE
                     TYLPQEPEEKNSVTLRQRSSWRDYLSQNMSSVSETVLAVTFIPFLVYFMLSWQDHVRA
                     ASVMLFKMENRNTAYVTLGLIAAMIRSFIVGNFIIGLFLSAASMAAFGLMGLPYFYFL
                     GVLSGFLSLIPYLGVILAILPPVIVGLGYLTTATFVLTIAIVIGLHLFAMNVLYPMVL
                     GKRLQLNPLAVTISVLFWGWLWGAMGLILAVPLTGALKIICDNIDRLRPYGAWMGE"
     gene            15799..16260
                     /gene="ruvX"
                     /locus_tag="VFC15_19390"
     CDS             15799..16260
                     /gene="ruvX"
                     /locus_tag="VFC15_19390"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZM12378.1"
                     /translation="METTSPSLEETRPSRIMAIDFGSRRIGLAVTDELGLTAQGLPTL
                     HRTNKQNDFNHLRRTIKQYAVAEIVMGLPLRMSGEHGIQSDKVENFAGELRKRFKLPV
                     HLFDERLTSVEANRVLSETDMSGRRRTEVVDQLAAVLILQAFMEFRSSHRD"
     gene            16321..17310
                     /gene="mltG"
                     /locus_tag="VFC15_19395"
     CDS             16321..17310
                     /gene="mltG"
                     /locus_tag="VFC15_19395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZM12379.1"
                     /translation="MRTFFTFIVLALLGFALWFYWAALSPVNPGETKYVLLYPGWSTR
                     HIAHTLQREGIIHSAPAFLMVHYAKGEGSLKAGEYKFDVPATALRVRDRVLRGDVFAR
                     TVVVPEGYNLYDIAAVVEQAGLGPAADFVAAAKSDLHMLRDIDPQAKSLEGYLFPDTY
                     QFTRIDTVNEIAAAMVHRFRQTAQKIGLLGRPDIHRIVTMASIVEKETAVAEERPLVA
                     SVYFNRLDRNMALGADPTVIYAALLGGRYRGTIYQSDLQFDSPYNTYKYPGLPPGPIA
                     NPGAASLQAAMHPAQSDFLYFVSDNNGHHRFARSAEEHDRNVAAYRKAVAAPR"
     gene            17406..18380
                     /locus_tag="VFC15_19400"
     CDS             17406..18380
                     /locus_tag="VFC15_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12380.1"
                     /translation="MKFSRLLIPAAFALLCSATGCLFHTRTVEVRVSNAKLQTATQQE
                     LIDRLNHDAAQIKTLSATVDIDTEVGGEKRGKVTDFKEIRGYVLVRKPNMLRMIGLYP
                     IVRNKAFDMVSDGQEFKLSIPASNKFYIGHNQSIQDSSNPLDTIRPQAIYDALLLREV
                     DPRNEIAVLESSTEMVVDSQNKKLVKQPNYVLDVIRKSDHGWTLARKVVFDRTKLRPH
                     QQITFDEKGNPVTEASYQVFKDYDGIQFPGLIDIKRPQEEYDIRLIMVKLTLNQPITE
                     DQFALQQPVGSILVNLDERNANAGAKQDGAAAATSAQSAPATNSAPPH"
     gene            complement(18470..18625)
                     /locus_tag="VFC15_19405"
     CDS             complement(18470..18625)
                     /locus_tag="VFC15_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12381.1"
                     /translation="MANTKSITDKIERKKVKRVARKKAAPKAPRTYARGSAKRKVKKM
                     VRGQSKR"
     gene            complement(18729..21314)
                     /locus_tag="VFC15_19410"
     CDS             complement(18729..21314)
                     /locus_tag="VFC15_19410"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZM12382.1"
                     /translation="MSYLSKLNPQQREAVETASGPVLILAGAGSGKTRVITYRIAHLI
                     DHVGVPPDSILAVTFTNKAASEMASRVESLVGVRTLAKPLIATFHSFCVRLLRRDIQT
                     LQIGGKGYRKDFVIYDETDQQTVVKASMKRLGIDHKQLTPRSVLSQISWAKNHMRDPQ
                     EVYLESGDPKTERVAQIYEVYKQELAKSNALDFDDLLLYAVRVLKASGETRERYNRRY
                     QHILVDEYQDTNRPQYELMRMLAGEEHNVCAVGDEDQSIYSWRGADIRNILEFEKDFP
                     DARIVRLEQNYRSTQTILQAASAVVARNIKRKGKNLWTERLGGAKVGYYEAPDGENEA
                     LFAADYIAKYLRQGIAENGEVPRAAVLYRTNSQSRLFEEAMRRYQLKYHVVGGFSFYE
                     RAEVKDLISYLKAIQNPDDSIAMQRIINTPTRGIGKNTTETLERVALETGLSLWGAIG
                     QAISPELLPPRALAALKGFKQLIDDARAMYLGSFRERMEETASPAQEAQQISEPPAEE
                     ADLQHAMDFSPEMFGEEDTTDFALAGFEEEGGGTGALSSPLAETEAAVEGFRSPGDGA
                     STAELLKFLIDRTGYIKQLEEEDTPDALARVENLRELVNAAMDSRDRGETLNEFLDHA
                     ALVSDADDYDENSRITLMTLHAAKGLEFPLVFLVGLEEGLFPHSRTLLAPDDIEEERR
                     LCYVGMTRAMDTLILTHARYRRRYGTDMPEASIPSRFLEEMPPELLQDLGTPRRGTPV
                     VAHDEGASTHYSYEDEDQRPQYGQPRSKPRSSYSGTSYNSIGNIADFFASRGKKFTLP
                     KTGPAEAPGRTGFRPGQRVRHPKYGEGMVYQREGDGENAKITVQFTKFGLKKLVEKYA
                     QLEKA"
     gene            complement(21393..21788)
                     /locus_tag="VFC15_19415"
     CDS             complement(21393..21788)
                     /locus_tag="VFC15_19415"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZM12383.1"
                     /translation="MELVADPKSVKVNKTAGTGMDIEWKDGHRSAFSFVFLRDACPCA
                     LCQEERDKDGRQPGDPLKPIAGALPMFKALARPTEVEPVGKYGIRFTWNDGHQHGIYS
                     WQFLRENCPCQECKAQRLMGAVATGSKPN"
     gene            21949..22491
                     /gene="cobO"
                     /locus_tag="VFC15_19420"
                     /pseudo
     CDS             21949..22491
                     /gene="cobO"
                     /locus_tag="VFC15_19420"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            22539..22904
                     /locus_tag="VFC15_19425"
     CDS             22539..22904
                     /locus_tag="VFC15_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12384.1"
                     /translation="MVDLSISGGSLELHVLGIDRLWALKSSLEIPLRHIAGIRADPEV
                     ARGWWHGIHMPGTDIPGVLTAGTFYQHGKRVFWDVHNPESTVIIELRDERYDELIVEV
                     ADPLFIVEQVKGALQPATK"
     gene            22931..23785
                     /gene="accD"
                     /locus_tag="VFC15_19430"
     CDS             22931..23785
                     /gene="accD"
                     /locus_tag="VFC15_19430"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZM12385.1"
                     /translation="MAWFKREQGDIDASGEKRVKTEGLWVKCDQCRQIIWKKELDQNF
                     NVCPKCDRHFRIDARTRLAYLLDDAKYETDDGNLVSNDPLKFTDLKSYADRLKRAKSD
                     TGLNDAIINARGKLNGKPVIVSAMEYAFIGGSMGAVVGETLTRAIERATAEKVPLIIV
                     SASGGARMMEGVVSLMQLAKISAALARMDDAKVPYISVLTDPTTGGTTASFAMLGDLN
                     IAEPGALIGFAGPRVIEQTIRQKLPEGFQRSEFLLQHGMLDAVVHRRELKNYISRALD
                     FMQAPKPS"
BASE COUNT         5059 a         7071 c         6771 g         4940 t
ORIGIN      
        1 tactacctca ctgccttggg caagcaagtc atcgctctcg gactcaaact caaaaacctc
       61 tacatcattc ccgcattggc agcggctgca tcctgaaagt tttgccagat tcggcaaaga
      121 cctgttgata taggtactaa accaacacaa aagatgcttg gttgcgagag gaatctggca
      181 aaatccgagc caagcaaatc gctaatgtcc aattgccaag tcccaattcc cgcctccaaa
      241 cgaccctgtg gaaagtttgt cgtcatcagt aattgcgtcg agggcggcgg cgggatattc
      301 tgcgaagcat cactccatgg cgaacctgtt cgaaccaccc cggtatatcg cagtcgaagg
      361 ccccattcgc gtcggcaaga gcacactgtc ccaggtgatt gcggaaaccc tgcatgcgca
      421 gcgcatcagc gagcctgatc gcaacccgtt tctgaagccg ttttataacg gcgaagcggg
      481 agcggccttc caggcacagt tcgcgttcct gattgcgcgc tacgaacaac tggcctcgct
      541 ggacgtgaaa cgcaatccca acaagacggt tgtcgccgac tacatcttcg agaaagacaa
      601 gctgtttgcc tgcgtcaacc tgaccgatgc cgaactcgaa gtttacaacc gttactacga
      661 gttattccgg cagaatgtgc ccacccccga tctcgtgatc tacctgcagg ccacgcctga
      721 ggtgctgaag aagcggctga agaaaaagaa cgcgccaggc gagaatgcga tcaacgatga
      781 ctatctcgaa gaagtcatca aggcctacga gcatttcttt tttcactaca cttcgtctga
      841 cctgctgatc gtcaacacct cggagatcga cttcgtggaa cacagtcaag atctgcaggc
      901 gctgctgcga cgggtcagcg agcctattcg cggaacccag tactttttgc cgctgggcag
      961 cgatgatgtg aagtcgtagg caatcgccgg tacctcgtaa cccgctgcca taaataatcc
     1021 cgatagcacc gattccaaaa tttcatctgc cattcgccaa ttcctgccca aaactgagct
     1081 aagcttcttc tcaccgcagg agggtgtcat gggagtcttg tttcaagctt tctattggaa
     1141 ctgtccagcc gccgaaaacc aggacggcac ctggtggaac ttcgtcgcca gcaaggtccc
     1201 tgagcttcag caggccggat tcactgcctt gtggctgccg cctgcctgca aggctgccaa
     1261 ccttggcggc ccgtcgatgg ggtacgaccc ctacgactac tacgatcttg gcgacgtcaa
     1321 ccagaagggc cgcgtgaaga cctggttcgg caaccaggcc gagctgacca cgctggtcga
     1381 cgccactcac accgccggca tgcaggccta cgccgacctg gtgatcaacc acaacaacgg
     1441 aggcgatgcg caggaactga acccgatcga cggcaacacg cgctggacca agttcaatcc
     1501 tggtagcggc aagttcgcgc gtgactggag aagctttcat ccatctccct acgaaacctt
     1561 tgacgaaatg tcttttggtg acatgccgga catgtgtcat cgcaatcccg gggtctacga
     1621 aaacctgatt aacctggcgc agttcatgat cgaaaccata ggcttcgacg gcttccgctt
     1681 cgatttcgtc aaaggctacg gcccgtggat ggtgaagtcg attgcagaac tgcgctacct
     1741 gaacaaagca gacggcgggt tctatccttt ctgcgtcggc gagtgctggg acaacgcgcg
     1801 aaccatcgat gactggctca cctccatcaa cacgtttatg gacaatccgg tttcggcgtt
     1861 cgactttccg ttgcattacc tccttaaggg agtctgcgac acttacggat tcagcctcag
     1921 cacgctggcc cagcccgccg gcacagtcgt tcaggacaca cccctgcggt ccgtgacgtt
     1981 cgtggacaat cacgacacca tccgcgacga cggaagcgcg atccttcacg acaagctgat
     2041 ggcttacagt ttcatcctga cccacgaggg ataccccagc gtgttctgga tggactggta
     2101 caacttcggc ctcgcgcaga cagggacatc gaacgggatc gccgccttgg ttaccgcgca
     2161 tgagcactac gctggaggag ctacccaggt gttgttcgca ggagacgacg tttacgtcat
     2221 gcagcgagtg ggcgacgcga gccacccggg cttggtctac gtactcaata accggggaga
     2281 cagctggaat ggagcgaccg tgcagacgca gtggaacaat gtgcgcttcg agccggttgc
     2341 ctggggaggt catgacacca gccgtcctgc caccaagtgg actcagagtg acggacacgg
     2401 cgacttctgg gccgggccga gaggatgggc ggtgtatgcg ccgcagttgc aagccacggc
     2461 gcggaccgaa agctagtgcg ctggcggcac gaagtctttc ggcttctcgg cgccttcgcc
     2521 gaagaagtac gcttccattt gctggacgat gaattcctgg tgttctttct tccagggttg
     2581 caggcggtac tcgttgagca gcatcttcat gtgctcctgc cattgctccc acgccagctt
     2641 ggagacgttg tcgtagatgc gctgtcccag ggggccgtcg aagggcggct cgtcgaggcc
     2701 ctccatttcg cgtcccattt ttacgcaatt caccatccgt gccatgcaga acccccaccg
     2761 ctatgcgcgg gataaatgcc aaccgacgat tctacgggaa ttggcgtgcg gaacgaagcg
     2821 gggttgccaa aggaaggatg ggatgggcta agcggacagt cgggctacta atggaatggc
     2881 gatttatcgc tcataatcgc gtcacttctc tcgatccact tagaaacagt acggtgcttc
     2941 acaggagggc gttcccgtta cctatgtggt gaaacttccc acaataagac ttcgggcccg
     3001 ggatagtttc cggttattcg gtcgggcgca atcccgactg aattgagcag cgacctgacc
     3061 ggatcatttg actaccgcaa ttcttagggc agttacctgt cggacccatc gtgctggccg
     3121 cttatcgccg ggagccacga ttcggaaagg cccttctttg gggtcaagcg gcttcccgtc
     3181 acgcttgtcc gcgaggatga tttcccgcgt ggcaaaggcc gggtcgatct ctgccagcgc
     3241 gaagactact ttatagccgt cggctgcttc taccagcagc atttcagtca accgcggtcc
     3301 acgcattttg tcacccaact gaacaccggc gcttgcaagc agggtcaata agggcacgcc
     3361 ttcaaattgg gcagggacat cgtgatcctt cacattcacc attacgtgtg gcgaactggc
     3421 gatctgcgcc acggtcaacg tggtcgatgc gccgtctgta tttatgatct gcagcgtttg
     3481 cgccgccgat ccggcgacaa aacagagcaa aaagcaaacc acacaaacta gcagaaactg
     3541 tcgttgcgtc attggatcct cacgctggaa aaacgggaat ttgccttcgc ttgatgacgc
     3601 ggctattctc tagccgaaca gatacttcaa tgccgaaact ctacactccc cgcgatgccg
     3661 cccaagtgct tggaatcagc tacgcttccc tgaagcagtg gatttaccag ggcaaactga
     3721 agagcgttca aacgctgggc ggtcaccatc gcattcccga gtcagagatt gatcgcttgt
     3781 taccgcgcgt catcgtctct ggcaccccgg agaagcgccg ccgcaccttt cgccgcatca
     3841 gcggaagaaa tcagttggtg ggccggatca ctgacgtcaa gatcagcggc ttacttgcac
     3901 aagttacgct ctcggtaggc ggccagcaca ttacctccat catcaccgcc gatgcggcgc
     3961 gcgagttacg cctcacaaaa ggcatggtcg ctgccgcgct catcaagtcc acagaagtca
     4021 tgatcatcat acccgactaa atcagattga caattacgat taaattcgat taaatcgacg
     4081 gaatctttgc gcccacgagg tgccgcgggc cacgcacgct acgaatcggt ctggcctcca
     4141 tattggggga aataaatgtc tgccagattc cgtctgcacc tcgtcggggt gtccctgttg
     4201 ttcctgtgcg cgctttccgc aagagcgcag gtgtcagcga gcatatccgg ccgggtcacc
     4261 gaccaaaccg gggccgttgt ggccggcgcc accgtaaccg cgaagagtct ggacaccggc
     4321 gtctcacgct ccacacccac cgatcaggcc gggcgctacg aactgattgc gctttcaatt
     4381 ggccagtttg aagtgcgcgc cgcaaggacg gctttgcgga acgcgtccgc accggcatcg
     4441 ttctcgtggt gggtcaggac gctaccgtcg atttgagtct gcccgtcggc aacgtcagcg
     4501 aacaggtgac ggtcgaaggg gacgcctcca tggtgaacgc caccacccag gacatctccg
     4561 gcctcatcgg cgagaaggaa gtcaaagagt tgcccctcaa cgggcggagc tatgacttgc
     4621 ttctcaccct gaaccccgga atcgtcaatt tcactggaga aaagaccggc ggcatcggcg
     4681 tttcgaattc gaccactggt aacaacttct ccctctccgg caatcggcct caacaaaacc
     4741 tgtttctact caacggcgtg gagttcaccg gcgccgctga gaacaacatg cagccgggta
     4801 gtcccagcga gaacttaatg ggcgtcgagg ccgtgcgcga attcaacgtg ctgcgcgact
     4861 cctacggtgc cgaatatggc aagcgtccgg gtgctcaggt aaccatcgtg acccagtcgg
     4921 ggactaacca ggtgcacggc tcggtttatg aattcctgcg caacaacgca ctggacgctc
     4981 ccaactattt cgaccaggga tccgcaccgc cgtttcaacg caatcagttc ggcgctgctc
     5041 taggcgggcc catccagaag aacaagacct tcctgttcgc aaatttcgag ggattcatcc
     5101 agaacctgca tcagacctca gtcgcattcg tgcccgatct cgcttcgcgc gcgaaagcag
     5161 tgccgagcgt tcaaccgctg ctgaacttat ggcccacacc ttcgccaacc gatcccgatt
     5221 tcagcggaat cgcgcaggtt tccagcagcc cgttacaaac cattcgcgaa tacttcggca
     5281 ccgcccggct cgaccacaac ttctcggcca acgacacgtt ctcggcggtg tacacgatcg
     5341 acgacggtgg cgacgttacg gcgacgacga tcaaccctta cagctccgac atactgaatt
     5401 tgcgcgagca ggtcgccacc ctggaagaga cgcacgtttt ctcgtccaca ttgttaaaca
     5461 ctgctcgttt tggtttttcg cgggcaggct acttcttcac gggcgaacct acgcccggca
     5521 cgccggccgc caccgtcccg ggatttctgg tcgggaagca agtaggcgca gtggtggtgg
     5581 gtggaagcgc agcgtcgaac cctcaagcac aaatcggcct ggccggcagc aacaacggca
     5641 gcaacctgac gatggcccgc aatctgtaca cctacgaaga ccgtgtgagt tggacccacg
     5701 gcgcgaacca gttcagtttc ggcgcgtggt tccagcaatt tcaatcgaac gagaccatcg
     5761 cactcagcca gtatggacag gcgacgttta ccagcctggc gacatttctc accggcgtca
     5821 ccagcacgtt tctatacgac cccgcaccga cagagatgaa ttggcgctct ctgttcgggg
     5881 cgctctatgc gcaagacgtc atccgggtca gcccaaactt caccttgaca cttggcttcc
     5941 gcggcgaatt ctccaccggc tggaatgagg cccatgggcg cgccgccaac tacaccttca
     6001 ccaatggagt gatttcgaac cagccgcgca tcgccggcgg cgccttcaca accaacaacg
     6061 cgaagtttct gccgcaaccg cgaatcggct tcgcctggag cctctaccct ggcaagaccg
     6121 tggtgcgtgc cgggttcgga atgtacaacg aactccagga tgcgttgggc tatcgcatgg
     6181 accagaatgt accgttcaac ccgacataca gtattgccgc gcagaccgtg gccaaactcc
     6241 cgattgaccc gaacgccccg gttccgccga aagcgctgct ggttccaggt ggagttcaac
     6301 cgaacctcag catgccgacg ctgatctcct acacgctgcg agtcgagcag gaactcactc
     6361 cgaatgcgac tctgactctg ggttacgtcg ggtcgcatgg ctatcatgaa atcgtcggcc
     6421 tcgacgccga cacacccata ccgacgatct gcccggcttc gccctgtcct gctaactacc
     6481 cgaaaagctt cccagcgggg ttggcgggaa cgccggttcc tgcaggctcc tactacattc
     6541 cgctaggaac gccgaaggcc aatcccacat tggccaatac ctggacttgg ttttcttacg
     6601 gcaccagtta ctacaacgcg ctgcaggtcg acctcaacca tcgcttcagc catggttttt
     6661 cgcttcgtgg cgcttacacc tggtcgaagg ctcttgacga cggagactca gtgaatgcca
     6721 cgacggctgg caacgccccg ggactggtgt ctaaccccta cgacataaaa gcagactggg
     6781 gtccggccac ctacgatgtc aggcagatcg gggtgatcag cgcagtgtac gacctgccgt
     6841 tcgggcgcgg ccaagcctac gtgaactccc tggatggcct ggcaaacggc ttgatcagcg
     6901 gatggcaggt gaacagcatc attaccttgc agtccggatt cccgttcacg ccgcagctca
     6961 gctacaatcc ctcgaacaac ggagacaccc gcaatccggt gcgcccgttc gctaatccca
     7021 atttcaacgg aaacgcgatt ctcggcaacc catcgcagtg gttcaacccc agcgcgttct
     7081 tgcagcctcc gcccaacagc ggcttctatg gcaatctcac gcgagataac ctgactggcc
     7141 cgggtctcgc cacatgggat ttctccgcta tcaagaacac gacaattcac gagcggctga
     7201 gcctgcagtt tcgcgccgag attttcaacc tgttgaaccg ggccaatttc aacacgccca
     7261 gcctgattgt cttcacaccg acaggggtct caggcacagc gggagcgatc acgagcacat
     7321 cgaccaccgc gcgtcaggtg caattcggct tgaaacttct ctggtagaaa ctgcgatcag
     7381 tctttctgga gcatcacttc ggtcgacttg attacgacct tgacggcgtc gcctttctcg
     7441 agcgccatag cttccgcgct tctccgggta atcacgctct cgatgaggtt ttcccccact
     7501 cgtaccacga cgtgtgccat tacgccgccg agctgaacct cctcgacggt acctgctagc
     7561 tgattgcgag ctgaaagtgt catgccgcaa gattaattca ggcgccatcg ccacgggaag
     7621 cggtctgccg tggcccatcg ccagacctaa ctcagaacac gaacttcagc gcgaactgca
     7681 cctggcgcgg atcgcgggct gccaggattt ggttgaacgt tggcgaactg atgtcgctgt
     7741 caggcaggcg gaaattcgga tgattcagga tattgaacag ctccgcccgg aactgcagcg
     7801 acatcgattc cgccagcttg atattcttca acaccccgaa gtcccaatcc atgtagccgg
     7861 gcccggtcgc gatgttacgc ccagcggtgc caaactgtcc cgcatcgagt gtggggtcca
     7921 ggcgcacgaa tgcactggca ttgagccagg cagtgaccga gtgagggcca ttgttcggat
     7981 tgcccaccag gtcaggacgc tgtgccgaaa aaccggtgat ctcaggcgcg ctgccctggg
     8041 ccgccacgtc attcgaatcg aacaccgtga acggcgtgcc ggtcatcagc gtaacgattc
     8101 cgctaagctg ccatcctccc aacgcctgct tgtaccactc acgcccgttc agccagaagg
     8161 gaaggtccca cacatagctg aagacgaagc gatggcgcgc gtcgaacatc gagaggccgc
     8221 gctcggccgc caagtcgaag gggttctgcg ccaggtcgtt ctcgcccgcg accggtttcg
     8281 cggccgagcc ggtcatattg aacgacgacg cgtcatcgat ggccttcgac cacgtgtacg
     8341 acgcgaggaa tgaaaccccg tgactgaaac gcttctgcag gctcgcctcc agcgcgttgt
     8401 acgaggagtt cgcgatgctc gcgatctcac ccatggagga gaaaatacaa ctgctcggcg
     8461 gatcgttgag agtgcatccc gaatagagcc gccgctggtt ggcgttgcca gaattcgaga
     8521 tgggctggcc gttgacgtag cccggcacaa acaccgccgg gtttccttcg atgaagcgcg
     8581 gcagatgcgt tcccttggtg ccgacgtatc ccatttcgaa caggaagtcg cgaccaaacg
     8641 agcgttgcac gttcaggtcc cagtcctgcg agtaaggcag tgtcatgttg ggcgccaggg
     8701 tcaggttggt cagtggggtt gagaacgagc cgggtatcgg cggattgccg ttgaacggat
     8761 cggcaaaatt cggcaagctc acctgcggcg tcgccaggta tggcggtgcg ctgatgggcg
     8821 tctgaagcgg gccgccctga ccggtgtaat aaggctcgta gaagatgccg tacgccgagg
     8881 taacaagcgt cttgccatcg ccaaacacat cgtaggcaat gcccacgcgt ggagcaaacg
     8941 cggttttgtc cgtagggatc agccccgcgg ggatgcccgg atcgccggga tacagcagcc
     9001 ctctgggagc gtcgggcatc accaccgact ggcgccccgg gataaacatc gactggaggt
     9061 tattcacctc tgtgtcgggc atcggaaggt cgtaacgcaa gcccaggttc agcgtgaact
     9121 tcgccgtcac cttataggtg tcctgcgcgt agaagttcag gtcgttgccg cgaatgtggc
     9181 gggtgaaatt gccaagcccc tgtaggaaga ccaccggcgt gcccgacagg aagctggcaa
     9241 acgcgtcggt tacggggaag ggcgcgaaca cgaagaagcc gttggtggcg atgccctgcg
     9301 taacattgat ctgctgatac tgatatccgc cgccgaactt gaggttatgc ttgccgtgtg
     9361 accagctcag tgatcccgag tagtcgaaca ggttctcata cgtattgcgc ggcccggtga
     9421 tggggttgcc gatgttggta tagccgctga tctggatgaa cggcggtcct tccgcaaccg
     9481 gcaggctgga ggaatactga aaacccaggc tttcgggagt cgtatgcacc tcgctctggc
     9541 catagaggaa cttgttccgc aagaacgaga accgcagtac cccgactaaa ttcggcgtga
     9601 acaggtgcgt ctcctgcgcg acaaaattct gtgctcgctg gccctcgccg accgggaagc
     9661 cgggcacgct ggctccttgc ggcgacagcg gattgacgtc tgacgactgg taaaacgagt
     9721 aacggaaatt cagattgtcg tgttgcgtga ggtagtcgtc gaccttgatg ccgaactggt
     9781 tgtagtcgtt gttgatctcc cccgacgcgg aatacaagtt gttccccaca ttcggtgccg
     9841 gaaaatactg cagcaggttc tgcgagacag gattgatcga cggcaattgg ttgtatggat
     9901 acggcgcatt ggcgaggacg ttgaacagtt ggttcgccgg attgttgcaa aagccgtggg
     9961 tgaatccctc cggacacaat tccgcgaagt tgccggtgcg ctcttgcaaa gaaggcacgg
    10021 tggtcagcgc cgtcacgcct tgcaggttgc gaaacccttc gtagtagcca aagaagaaga
    10081 ttttgtcctt gcggatcggt ccgccgatgg ttgcgccgaa ctggttctgc ctcagtggca
    10141 gggcctgcgt ctgaaaatag ttacgcgcgt cgacagcatc attgcgcagg aactcccaca
    10201 gcgcgccgtg gaagtagttg gtgcccgagc gcgtgacgat gttcgtcgtc gagcctaagg
    10261 cgtcgccgaa ctcggcgctt gcattgtgcg tgataatgag gaattccgaa attgcgtcca
    10321 ccggcggctt cagcacataa ccgccatcga cgccgttaaa gttatcggcg ccgtcgatca
    10381 ggaaattgtt cgattcgggc cgctgcccgt ttaccgagta cggctgtccg tcacgcaatc
    10441 cgccgcccgc ctccactaac cccggcgtta tcggtaccac accgggctgc agcaatccca
    10501 gctgcgaaaa attgcgcccg ttaagcggca ggtcttccag ctcccgctca cccacgacct
    10561 tgcccatggc ggttaccccg ggttcaatga ggtcggcatc ggcggtgacc tgcaccactg
    10621 ctatttccga tccgactaca agatgcggcg aaaccgaggc ggtttcgttc acgttcagcg
    10681 agatgccgtc ttgcacgtat tcctgaaagc cttttgccgt gacttgcagg cgataatgtc
    10741 cgacgggaag ttcaagcaca acgtagttgc cagtgcggtc ggtggttacc gtgcgcgaaa
    10801 gaccggtttc ggattgccgg gctgtaacct ttgcgcctac aacgattgca cccattggat
    10861 cggtgactgt gccccgaatg ctgcccgtga tctgctgagc aggcaaactg aggcaagcaa
    10921 gcactacaaa tatcagcaca ttgcgaatga cgttcatgtg gttttcgagt ctcccagcag
    10981 atttggtagt agacaaacgt ctttatgcac gccgaaaggc gtacgaatca ccggttcgcg
    11041 tttcgcgagt tgcccccaaa cagaatgtcg cgattatagg caggtaaaaa tgatgatcaa
    11101 taataggaaa gaactaatag gcagcagttc ccgagattac gagacgagta gaagacgatc
    11161 cggcagcatt gctactcgcc gttttgcaag tctggccaat cgtggcccct gtacggaagt
    11221 gctggaggag accagtcagc ttccaccaca gcttctccag atcgaggtcg cactataagt
    11281 tcgaaccgaa agccagcaaa cggccgtcgc aatccttcac tacaaattct cgcgagtgcc
    11341 atggcgtgtt ggcaagttcc cgcacgaact ccaacccttg ggctgcacgc gattcacttt
    11401 cgcgctgagc cgcccaccgc caatccggac aaatatgcgg acgaactgct cgacgcatat
    11461 atctccgtcg aagatccaga cgcgctttat gccgagtacg atcgcgggct acgatggcgt
    11521 ataccgtgga tcctgccacg ttcccagaca ctcgaagcca agcttgtcct tgtaataggc
    11581 gagcgtgccg ggaatgtcgg tggtgaagaa ttgaggtacg atctgacgga tcatggatca
    11641 agtgtacggt aacgcctgcg ggacgatcgc ctgcgaagtc gtgggcccta agttggacgg
    11701 ccgaagaaca aaacgtagcc gtccgggtcg caaatctcca cggaaattgg cgtgcggaac
    11761 gaagctgggt tggccgaagg aaagggcagg gatggctgag caaatcggca cagacgacag
    11821 aacgtatttg atcgagactc ctaccagagg tactacaaat gacgcaatcc caggcgaccc
    11881 gaatcatcat cgacactcac cgacacattt tcggtccgaa actgcgtcag aaattcgttg
    11941 agaatatagg ctttgatgac tcaaagccgt tgccgcaggc caacgcgggc gaaatgtttt
    12001 tctttgtttt tctatcgcga gtcggtcgac gttgattatt cgatggagat tcagcgacag
    12061 agtggggtta cgctctgcgt gaacagctat ggcggcgaaa tagagacgtt cgcccagaac
    12121 atcattaaag caagcaccat cgacacgctc aaattcctgc acgacgagtc cttcgagctt
    12181 cgagatagat tcccccagga cattgcctgc atggccaacg ctcatgctct ggaagagaat
    12241 acgcgtgatg tcattgatcc gctgatctcc aacaaagaag cgtgctgcat cagcatttcg
    12301 accagctacg gagccggtcc cgatcagatc tttctcgact cgcccaaggc cgaatggcta
    12361 tgggagtacg cgcaggacaa ggatgttctg gttcatattc atccacccct ggtagcgata
    12421 ggcgctgctt ccatgcagca gtacaggctt aacgaagccg taggccggcc atttgatacg
    12481 gcgctgtcgg cagctcgcat gatctatagc ggcgtcttcg ataaataccc aaagctcaag
    12541 gttcttttcg ttcacatggg cggagccctc gctcccgtga tctgcagact cgattggaac
    12601 tgggagctaa actataaagg catccagaac ccgccgatcg acaaggtcga taagaatctt
    12661 cgtaagccct cggagtattt caagtcaaat atctatgtgg atactatggg tccaagcgcg
    12721 atctgcctca aggccatgat cgaaatgtga ggcattgacc gcgtactgtt tggaaccgat
    12781 ttcgggccgg ttccaatgag cccaaagctg cacatcgatc ttgtagacga cacgatcacc
    12841 gacgctggag atcgcgaaaa gattttctca accaacacac ttgctttgct ccgtttggct
    12901 gaaagtgtac ctcgcctcac cccccgagct acttagtctt acgccaatgc gtacaggtat
    12961 tcgacaaacg atcccactgc accatcaagc cccgcaacct tcggattggt ggagtcgatc
    13021 aggtagtact cgttaggagc gtgggcgccg gtgccgtgac cgaggccgaa gtgtccggcg
    13081 ggcagactca gtggcggatt ggtgaaaatg tatcccggcc atgaacctgc ggcgcgcggc
    13141 cacaacaagg gatcctggcc gagcttctta taggtcgcca tctgtgcctg gatgagcttg
    13201 gtgtcgggcg aagtctggtt gggatcgtag cctccgctca tgttgacctc gatatcgccg
    13261 aagcctttct tcgccaaata gtctttcagc aacgccaaag tgccggaggc ggtcatatcg
    13321 ggcacgagcc gcatgtcgat cttggcgacg gcacgatgcc ccaggaccgt cttgccgccc
    13381 ggcccggtgt agccggcgac cagcccttcg atgttgatgg tgggctgcga ttccagggcg
    13441 atcatggcgt ccagccactg cttgtcgccg gcccagtgct cgacgccgag ctgctgcttc
    13501 atggtggctt cactgcgccg ggccgcgact gcttcgacca tcttcacctg cacgggagtc
    13561 agcggcttgg ctttgtcgaa gaagcctgtc accgcagggg tgtggccgtc gggctggatc
    13621 agcgtgttca aggtctgcac caggtgccac gtaggactgt cgacggtcgc ttccaggctg
    13681 gagtgaacgt cgtgcttgtg cccgcctccc cacttttcgc cggtcgaaac caactccaac
    13741 tcgaccacgc ctttggcgcc gaggtcgatc tcgacgtttc cgtcggcttc ctgtcctgcc
    13801 gaaggcatga agacgcccat ggatttctgc agcgcggcca tgaccttggg attcttcacg
    13861 atctccggaa agtgcggcga accaatctct tcttcgcctt cgcaggcgag tacaaggttt
    13921 acgggcagat ttactttcgc ctctttgaaa gcgcgcagag cgctgaggaa cgcagtctcg
    13981 ggacctttct ggttcactgc accgcgaccc atgcagactt tgcccaaacc ttgcttgtcg
    14041 acaatcttgc cttccagcgg aggcgaagac cattccgcgg gatcgaactg ctttacgtcg
    14101 tacatgaagt aaatgccgac ggtggtgggc gcgcccgcat taatgactcc gaagaccccg
    14161 ggcttgcctg aggtgggaat gatctcaacg tcggtgaagc cagcctcgcg cgccagcttc
    14221 gccatatact ccggcccctg tgggtagttg cggttctcgg ctgcgatcga cggcagcgcg
    14281 atccattcct gcaagtgctt gacgttttcg tcttgcatct tcgggatttg ggccagcacc
    14341 gtttgcatgt cggggttagc ggcagcgaat gacgggatcg gtagtgcgga gagcgcggct
    14401 cccgctagtg cgccctggat aaactcccga cgattgaaag ggctattgct cgattcggtc
    14461 atggcgtctc ctcttgttat gtcggtctac gaggtccaga tactgacagt attcaggtgc
    14521 gacgcggacg cttgatggcg cgcatccgct gagttggttg gcggctattc gcccatccat
    14581 gcgccatacg ggcgcagccg gtcgatattg tcgcagatga ttttcagggc gccggtcaac
    14641 ggtaccgcca ggatcagtcc catggctccc cacaaccatc cccaaaacaa cacggaaatc
    14701 gtgaccgcca ggggattcag ttgcagccgc tttcccagca ccatgggata gagcacgttc
    14761 atggcgaaca ggtgcaggcc aatcacgatg gcgatggtga ggacgaaggt tgcggtggtc
    14821 aggtagccaa ggccgacgat tactggtggc aggatggcga gaatcactcc caagtatggg
    14881 atgaggctaa ggaacccgct caggacgccc aggaaataga aatatggcaa acccatcagg
    14941 ccaaacgcgg ccatgctggc agcactcaag aacagcccaa tgatgaagtt ccctacgatg
    15001 aacgagcgga tcatcgccgc gatcaggccc agtgtcacgt aggccgtgtt gcggttctcc
    15061 atcttgaaca gcatgaccga cgcagcccgt acgtggtcct gccaactcaa catgaaatac
    15121 accaggaagg gaatgaatgt gaccgccaaa acggtctccg acaccgagct catgttctgg
    15181 ctcaagtagt ctcgccaact gcttcgctgc cgcaaagtca ccgaattttt ttcttcaggc
    15241 tcctgcggca agtaggtctc ggtggtcttc tcaaactgct cggcctgctg cctgatctcg
    15301 tccacgatgt gtttgagccg gatcttgtat tccggcatct gcatcatgaa ctcgaccgcg
    15361 cgggcatagg agagatagga gacggtaccg atcgaggcca cgagaaggaa caccgcgatc
    15421 aaggatccca gcggacgcgg gacccggaaa tttgcgaggg catcggccac tggcgccaaa
    15481 acaaaggcca gcaggaccga tgtcaggatg accaccagga tcagcttggc gacgtacaac
    15541 aagctgagga ccgcgccggt tgccaggatc accagcgccg ccgcctgtac tcgttggatg
    15601 cggtgctgct ccaattccgc gctggacggc cgttccggag ccgcccgctg cagcggttgt
    15661 ggaccactct gctctggact ggggaacacc tcagacccgc ctggcacgtt gatgtcgtcg
    15721 atcagtgggt cagccatctc gcctatggta ctcgcacgac ttagcaagtt cgaattagga
    15781 tacccttctg gaatcaatat ggaaactact tctccatcat tggaggaaac ccggccatcg
    15841 cggatcatgg ccattgattt tggctcgcgg cggattggtc tggccgtgac ggatgaactt
    15901 ggtctgacgg cgcaaggcct gcccacactg caccgaacta acaagcaaaa cgacttcaac
    15961 catttgcggc gaaccatcaa acaatatgcg gtcgcggaaa tcgtgatggg actgcccctc
    16021 cgcatgagcg gagagcatgg aattcagtcg gacaaagtcg agaatttcgc cggagaactg
    16081 cgcaagcggt tcaagctgcc ggtccacctg ttcgacgagc gcctcacttc cgtggaagct
    16141 aatcgggtgc tgagtgagac cgatatgagc ggccggcgca ggactgaggt tgtggatcag
    16201 ttggcggcag tgctcatttt gcaggcattc atggagtttc gcagctcaca ccgggattga
    16261 gccccgcggc tcgccaggca tccgctaaac ttctgacagt cggtagcatc taatccattc
    16321 gtgcgaactt ttttcacatt catcgtgctg gcgctgctgg gattcgctct gtggttttat
    16381 tgggcggcgt tgtcgccggt caatccgggt gaaacgaaat acgttttgct gtatcccgga
    16441 tggagtaccc ggcacatcgc ccacacactg caacgcgagg gcatcatcca cagcgccccg
    16501 gcgttcctga tggtgcatta cgcgaaaggc gagggcagcc ttaaggctgg cgagtacaaa
    16561 ttcgacgttc cggcgactgc actgcgggtg cgcgaccgcg tactacgcgg ggatgtcttc
    16621 gcccgcaccg tcgtcgttcc cgaaggctac aacctctatg acatcgcggc cgtagtcgag
    16681 caggctggcc tgggacctgc tgccgacttt gttgccgcgg cgaagagtga tttgcacatg
    16741 ctgcgcgata tcgatcccca ggcaaaatcg ctggaaggct acctctttcc ggacacctac
    16801 caattcacgc gcatcgacac cgtcaacgaa atcgcggccg ccatggtcca ccggttccgg
    16861 cagacggcgc agaagatcgg cttgctgggc aggccggata tccaccgcat cgttaccatg
    16921 gcgtccatcg tagaaaagga gactgcggtt gcggaagaac gccctctcgt cgccagcgtg
    16981 tacttcaacc ggctggacag gaacatggca ctgggagccg atccgacggt tatttacgca
    17041 gcattactgg gggggaggta ccggggcacg atctaccagt ctgacttgca gttcgactcg
    17101 ccttataaca cctacaagta tcccggattg ccgcccgggc caatcgccaa tccgggagct
    17161 gcctctctgc aggccgccat gcatccggcg cagagcgatt tcttgtattt tgtgagcgac
    17221 aataacggcc accatcgttt tgcgcgtagt gcggaggagc acgaccgcaa cgtagccgcc
    17281 tatcgcaagg ccgtagcagc gccacgatga tcatttcatt gtgggccgct gcttgctgca
    17341 atcggcacga ccgccatggt ccgagctata ctggatggtc tggataagca attggcccgt
    17401 cctgtatgaa gttttcccga ctcctaattc cagccgcatt tgcgctctta tgcagcgcca
    17461 ccgggtgcct cttccacacc cgcacggtcg aggtacgcgt cagcaacgcc aagcttcaga
    17521 cggccaccca gcaggaactc atcgatcgtc tcaaccacga cgcggcgcag atcaaaaccc
    17581 tcagcgcgac ggtcgacatc gacaccgaag taggcggtga aaaacggggc aaggtcactg
    17641 acttcaagga gatccgaggt tacgtactag tgcgcaaacc aaatatgttg cgcatgatcg
    17701 gactctaccc catcgtgcgc aacaaggcct ttgacatggt cagcgacggt caggagttta
    17761 agctttcgat tccggcgagc aacaagttct atatcgggca taaccagtcg atccaagact
    17821 cttccaatcc cctggacacg attcgtccgc aggcgatcta cgatgccttg ttgctgcgcg
    17881 aagtcgatcc ccgaaacgaa atcgcagtgc tggagagcag cacagagatg gtggtcgact
    17941 cccaaaacaa gaaactggtg aagcagccga actacgtgtt agacgtgatc cggaagagcg
    18001 atcatggatg gactctggcg cgcaaggtcg tgttcgatcg cacaaaacta cggcctcacc
    18061 agcagatcac ctttgacgaa aaggggaatc cggtcaccga ggccagctac caggtcttta
    18121 aggattacga cgggattcag ttccctggct taattgacat caagcggccg caggaagagt
    18181 acgacatccg cttgatcatg gtgaagctga cgttgaacca gccaatcacc gaggaccagt
    18241 tcgcgttgca acaaccggtc ggatcaatac tagtcaacct cgacgaacga aacgcgaacg
    18301 ccggggcaaa gcaagacggc gcggcggcag cgacctcggc gcaatccgcg cccgccacaa
    18361 actctgcacc tcctcactaa ctgcccttcg cgccttattc ttgtcggcag caaaaaaggc
    18421 ccaaacccgt cgagcgggct ggagccgtgg tgttttctac ggcgggtacc taacgcttcg
    18481 actggccgcg taccatcttc ttgaccttgc gcttggccga tccccgggcg taggtgcggg
    18541 gcgctttcgg ggccgctttc ttgcgggcta cgcgctttac cttcttgcgc tctattttgt
    18601 cggtaatgct cttggtattt gccatgaata ctgcgttcct ttgcgatctt ggattcgaag
    18661 ggggacgctt gctagcgcct gggagcccga tgctctggac tgcaatcctt cgctttaatg
    18721 cgcgagactt acgccttttc gagttgggcg tacttctcca ccagcttttt caagccgaac
    18781 ttagtaaatt gtaccgtaat cttggcgttc tccccatctc cctcgcgctg gtacaccatt
    18841 ccctcgccgt atttggggtg acgcacgcgc tgcccgggac ggaatccggt acgcccgggc
    18901 gcctctgccg gcccagtttt tggcagcgta aacttcttgc cgcgcgaagc gaagaagtcg
    18961 gcaatgttgc cgatcgagtt gtacgaggtc ccggaatacg agcttcgtgg cttgcttctg
    19021 ggctgtccgt actgcggacg ctggtcttcg tcttcgtacg agtaatgcgt cgacgcccct
    19081 tcgtcgtggg caactacggg agttccgcga cgcggcgttc ccaagtcttg cagcaactct
    19141 ggcggcattt cttccaggaa ccgcgaggga atactcgcct caggcatgtc ggtgccgtag
    19201 cggcggcgat agcgggcgtg ggtcaggatc agcgtgtcca tcgcccgcgt cataccgacg
    19261 tagcacaggc gccgctcctc ctcgatgtcg tcgggcgcca gcagcgtgcg tgaatgtggg
    19321 aacaggccct cctccagccc cactaggaac accagcggaa actccagtcc cttggcggcg
    19381 tgcagcgtca tcaacgtgat gcgcgagttc tcgtcgtaat cgtcagcatc gctgaccagc
    19441 gccgcgtggt cgaggaattc gttcagcgtt tcgccccggt cgcgtgagtc catggcggcg
    19501 ttgaccagtt cgcgcaggtt ctcgacacga gccagcgcgt cgggcgtgtc ttcctcttcc
    19561 aactgcttga tgtatcccgt gcggtcgatc aggaatttca gcaactcggc cgtcgaagcg
    19621 ccgtcgccgg gactgcggaa gccttcgacc gctgcttcag tctcagcgag cggcgagctt
    19681 agggcgccag tgccgccacc ttcttcctcg aacccggcaa gagcgaaatc ggtggtgtct
    19741 tcctcaccga acatttccgg tgaaaagtcc atggcgtgct ggaggtctgc ttcctcagcc
    19801 ggtggttcgg agatctgctg ggcttcctgc gccggagagg ctgtctcctc catgcgctcg
    19861 cgaaacgacc cgagatacat cgcgcgggca tcgtcgatta gctgcttgaa gcccttcaag
    19921 gccgccagtg ctcgcggcgg cagcaactcg gggctgatcg cctgtccgat cgcgccccac
    19981 agcgatagcc cagtttccag tgcgacccgc tccagcgtct cggtcgtgtt cttcccgatt
    20041 ccgcgcgtgg gagtattgat gattcgctgc atggcgatcg agtcgtcggg attctggatc
    20101 gccttgagat acgagatcag gtccttgacc tcggcgcgtt cataaaaact gaagcctccc
    20161 acaacgtggt acttcagctg gtaacggcgc atcgcttctt caaacaggcg ggattgcgag
    20221 ttggtgcggt acagcactgc ggcgcgcggc acttcaccgt tctcggctat accctgccgg
    20281 agatacttcg cgatgtagtc ggcggcgaac agggcctcgt tctcgccgtc gggagcttcg
    20341 tagtagccaa ctttcgctcc gcccagacgc tcggtccaaa ggtttttgcc tttgcgcttg
    20401 atatttcgcg ccaccacagc cgaagctgcc tgcaggatgg tttgcgtcga acggtagttc
    20461 tgctccaggc ggacgatgcg cgcgtcgggg aaatccttct cgaattccag gatgttgcgg
    20521 atgtcggcgc cccgccacga atagatgctc tggtcttcgt ctcccaccgc gcatacgttg
    20581 tgttcttcgc ctgcgagcat ccgcatcaat tcgtattgcg gacggttggt gtcctggtat
    20641 tcgtccacca ggatgtgctg gtagcgccgg ttgtagcgct cgcgggtttc gcctgatgcc
    20701 ttcagcacgc gcaccgcgta gagcagcagg tcgtcaaaat ccagggcatt cgacttcgcc
    20761 agttcctgct tgtaaacctc gtaaatctgc gcaacgcgct ccgttttcgg gtcgccggat
    20821 tcgaggtaaa cctcctgcgg atcgcgcatg tggtttttcg cccacgaaat ctgcgagagc
    20881 acgctgcgcg gcgttagctg tttgtggtcg atccccagcc gcttcatcga ggctttgacc
    20941 acagtctgct ggtcggtctc gtcgtaaatt acgaaatcct tgcgataccc cttgccgccg
    21001 atctgaagtg tctggatatc gcggcgcagc aatcgcacac aaaacgaatg aaacgtcgcg
    21061 atcagcggct tggccagcgt gcggaccccg accagcgatt cgacgcgact ggccatctcg
    21121 ctggcggcct tgttggtgaa ggtgacggcg aggatggagt ccggcggcac gccaacgtgg
    21181 tcgatgaggt gcgcgatgcg ataggtgatg acccgcgttt tgccgctgcc ggcgccagcc
    21241 agtatcagca caggtccact ggcggtttca acggcctcgc gttgttgcgg atttaacttc
    21301 gagagatacg acaattctta atttcctgaa atctccggtg gacggaagag tgcgtgttca
    21361 actctgtttt gaaagggcac ggcttcgcgc gatcagttcg gcttgctgcc cgtcgcgacc
    21421 gctcccatca accgctgggc cttgcactcc tgacagggac agttctcgcg gaggaattgc
    21481 cacgaataga tgccgtgttg atggccgtcg ttccaggtga accgaatacc gtacttgccc
    21541 acgggttcaa cctcggtggg acgcgccagc gccttgaaca ttggcagcgc gccggcaata
    21601 ggtttcagag ggtcaccggg ttggcggccg tccttgtcgc gttcttcctg gcagagcgca
    21661 caggggcagg catcgcggag aaagacgaag ctaaacgcgc tgcgatggcc gtctttccac
    21721 tcgatgtcca tgcccgtgcc cgcggttttg ttgactttga cggacttggg atcggctacc
    21781 aattccataa tcctgtgcct ctccgcagta atcctcgcgg gagctaactc tctagtatcg
    21841 cagaaaatcc gttactcaat cccaagctgc taggattggg gttccgccaa ttcctcccgc
    21901 agttccgcga gcgaggtagg cgaccattcc gcaaatcgga caggggtttc aatcatggca
    21961 gacgtacgtc gaggcctcat catggtgaac accggacccg gcaagggcaa aaccaccgcc
    22021 gccatgggga cgggcctgcg cgcggtcggc aacggcttga aggttctgat gctgcaattc
    22081 ctcaagggct cctggcatta cggcgaactc gatgccgtca aggctttcgg cgataatttc
    22141 gtgatgaagc aaatggggcg cggcttcgtg aaagtgggcg gagcggaaac cgatcccgaa
    22201 gatgtacgaa tggtggatga agcctgggcc gaagccgagc aggctattct ctccggagca
    22261 tgggacctgg tgatcctcga cgaaatcaac tacgccatca gctacaaaat gctcgatccc
    22321 gagaaagtcg tagccgcgct aaagaagaag ccagaaatgg tgcacgtcat cctcaccgga
    22381 cgtaatgcac atcccagcat cgtcgaaatc gccgacacgg ttaccgagat gaaggaagtg
    22441 aagcacgcct acaccaaggg catcgaggcg cagcggggca tcgaatacta agcggcgaca
    22501 actctcaccc cagtcggcta cactgaatcc tgacttggat ggtcgatcta tcaatttcgg
    22561 gcggcagcct tgaattgcac gtcctgggca tcgacaggct gtgggcgctg aagagttcgc
    22621 ttgaaatccc gcttcgtcac attgcgggaa tacgagctga tccagaggtg gcccggggct
    22681 ggtggcacgg catacacatg ccgggaaccg atattcccgg agtgcttaca gcagggacgt
    22741 tttatcaaca cggcaagcga gtcttttggg atgttcataa tcccgagagc acggtcatca
    22801 tcgaattgcg ggatgaacgc tacgacgagc tgattgtcga agtcgccgat ccgctcttta
    22861 ttgtcgagca ggtcaaaggt gcgctgcagc cggctacgaa gtgaagtcgt agaattgcga
    22921 agactaccct atggcatggt ttaagcggga acagggagac atcgacgcgt cgggcgagaa
    22981 gcgcgtcaag actgaagggc tctgggtcaa gtgcgaccag tgtcgccaga tcatctggaa
    23041 gaaggaactc gaccagaact tcaatgtctg ccccaagtgc gatcgccatt tccgtatcga
    23101 tgcccgcacc cgtcttgcct acttgctcga cgatgccaag tacgaaaccg acgacggcaa
    23161 cctggtctcg aatgacccgc tcaagttcac cgacctgaag tcctacgccg atcgcctcaa
    23221 gcgggctaag agcgataccg gtctgaacga tgccatcatc aacgctcgcg gcaagttgaa
    23281 cggcaagccg gtcatcgtca gtgcgatgga atacgcgttt atcggtggat cgatgggtgc
    23341 ggtggtcggc gagaccctca cccgcgcgat tgagagggcc acggccgaga aggtcccact
    23401 gatcatcgtt tccgcttccg gcggagcgcg catgatggaa ggcgtggtca gcctgatgca
    23461 actggcgaag atttctgccg cgctggcccg catggacgac gccaaagtcc cctacatctc
    23521 cgtcctcacc gatcccacga ccggcggaac aaccgcctcc tttgccatgc ttggagacct
    23581 caacatcgcc gaaccgggcg cgctgattgg cttcgccgga ccacgcgtga ttgagcagac
    23641 catccgccag aaactgcccg aaggcttcca gcgcagtgaa ttcctgctgc agcacggaat
    23701 gctcgacgcc gtggtccatc gccgcgagct gaagaactac atttcgcgcg ctctcgactt
    23761 catgcaggcc cccaaaccct cctagcactt gtgccccaga tcaccgtcgc ggattccctg
    23821 atgttttaca ctcacttcgt g
//
LOCUS       DASRKK010000809         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_872449, whole genome shotgun sequence.
ACCESSION   DASRKK010000809 DASRKK010000000
VERSION     DASRKK010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872449"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1503)
                     /locus_tag="VFC15_19435"
     CDS             complement(67..1503)
                     /locus_tag="VFC15_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZM12386.1"
                     /translation="MARFVVGDDRGQSTLFPERLEDYLSEDNPVRAIDVFVDELDLAG
                     LGFGGVEPEATGRPAYHPATLLKIYVYGYLNRVQSSRRLERECQRNIELVWLTGRLMP
                     DFKTIADFRKDNGEAIRRVCREFVVLCRRLELFGEASVAIDGSKFKAVNTRDRNFTQA
                     KMQRRLAQIDESIARYLSQLDSADRQGEAVPAAKITRLNEKIATLRQEIQRLNALNIQ
                     MMQTDDKQVSLTDPDARSMATSGRGSGMVGYNVQSAVDTKHHLIVAHEVTNVGSDRSQ
                     LSHMTERARAAIGSEAIDAVADRGYYNGEEIVACEQAGITVYLPKPMTSGLLAKGRFG
                     KQDFVYVAADDVYLCPAGEQLTYHYTNEEEGRTLRRYWTTACQACALKSKCTTGKERR
                     ISRWEHEAVLEAVQARLDRNPAKMRERRQTVEHPFGTIKSWMGATHFQMKTLKHVGTE
                     MALHVLAYNMKRVISILGVGGLMEAIRA"
BASE COUNT          484 a          706 c          680 g          536 t
ORIGIN      
        1 gcttttgacc cagactgtgt aaaaacgcga tttttgagca gggcaagctc gacatggtga
       61 cgccggtcag gcccggatcg cctccatcaa accaccaacg cccagaatgc tgattaccct
      121 tttcatgttg taggccagga cgtggagcgc catttcggta ccgacgtgct tgagcgtttt
      181 catctggaag tgcgtggctc ccatccagga ctttatcgtg ccgaagggat gctccacagt
      241 ttggcggcgc tctcgcatct tagccgggtt tcgatcaagc ctcgcttgca cggcctcaag
      301 gacggcttcg tgctcccatc ttgagatccg ccgctccttg cctgttgtgc acttgctctt
      361 cagtgcgcac gcctgacagg cagtggtcca atagcgacgt agtgttctgc cctcttcctc
      421 gttggtgtag tgataagtta gctgctcacc ggcagggcaa agataaacgt cgtccgccgc
      481 gacatagacg aagtcctgct tgccgaagcg gcctttggcg agaaggcccg aggtcatcgg
      541 tttcggcaga tacactgtga tgccggcttg ctcgcaggcc acaatttcct caccgttata
      601 gtacccgcgg tcggcaacag cgtcgatggc ctcagaacca atcgcagcac gcgcccgctc
      661 ggtcatgtga gatagctggc tccggtcaga cccaacgttg gtcacctcat gcgcaacaat
      721 gagatgatgt ttggtgtcga cggcactttg tacattgtaa ccgaccatcc cgctgcctcg
      781 gccacttgtg gccatcgagc gagcatcggg atcggtcagc gatacctgct tgtcatcggt
      841 ctgcatcatc tgaatgttca gagcgttcag gcgttgtatc tcctgacgca gcgtcgcaat
      901 cttctcgttg agccgggtga tcttggccgc cggtaccgct tccccctggc gatcggcgct
      961 atcgagctgc gagagatatc gggcgatgct ctcgtcaatc tgcgccagcc gcctctgcat
     1021 cttggcctgg gtgaagttcc ggtctcgcgt gttgaccgcc ttgaacttgc tgccgtcgat
     1081 cgcaacactc gcttcgccga acagctcaag gcgtcggcac agcaccacga actcgcggca
     1141 aacccggcgg atggcctcgc cgttgtcctt gcggaagtcc gctatcgtct tgaagtcggg
     1201 catcaggcga cccgtcagcc aaaccaactc gatgttgcgc tggcactccc gctctaggcg
     1261 ccggctcgac tgcacccgat tgaggtatcc gtagacgtaa atcttcaaca acgtcgccgg
     1321 atggtaagcc ggccttcctg ttgcctccgg ctcaacgccg ccaaagccta gccccgccag
     1381 atcaagctca tcgacaaaaa catcgatcgc ccggaccgga ttgtcctcgg acaaatagtc
     1441 ttccagccgc tcgggaaaca aagtgctctg accgcggtca tcgccaacga caaagcgcgc
     1501 catcgaatcc tcccgacaaa tcgcaggaga atcatatcat ttgaggggcg ttttcacaca
     1561 gtctggaccc ggaccggaca tagccgtttg aaaatgccaa cgcggagaac aggctactgc
     1621 tgctggatca gattttgcga aagcgaagaa tgagccggac ggctgagaaa tccagccggg
     1681 gattgccatt ccactggtgg cagcactatg attaaggtct ttcattcctc attttgcagg
     1741 agcattccat gtcacgcttt gtatttgcgc ttctctccct gacactcttt gttggattgg
     1801 actgcgtgtc ggaggcacag gcggccgccg ctgtttgtga cgtcaatgcg tgcataaaca
     1861 cgaggtgcaa gcgtgcaagg ggaagcagca tccagacctg caactcgggt tgtcagatcg
     1921 atgtggcgga aaatcagaag aagaagttgt gcaagtaagc cgccgcgcgg cctaccgccg
     1981 cggaagttcc gctaggctgc ctgatccacg atcatagggt ctgaagaagg gccgccttcg
     2041 ggcggctttt tgtttggcaa ctcgtgaagg cgcgacttct ggggttggag tttttcggac
     2101 atgccgcgat gtccgctttg agtccgctgt cgggggtaaa gcagactcta tctgctcgca
     2161 atgagcattt ccgcttttga cggcgagttc aaccgatcgc tgcaacacac tcagcgaact
     2221 tctcagccgg cgtctgatag agcaaggttt ttctcgagaa taaatgacgg tgagtggagt
     2281 cagacggcaa tatcctcccc aagtggaacg cgggtgacct ggtgagcctg cagcgcgggc
     2341 aggacctccc gggcaaaccg ccggatttgc tccagaaaca tttcttgcgg tagcgcgccg
     2401 cggttg
//
LOCUS       DASRKK010000810         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1068621, whole genome shotgun sequence.
ACCESSION   DASRKK010000810 DASRKK010000000
VERSION     DASRKK010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068621"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1261
                     /locus_tag="VFC15_19440"
     CDS             173..1261
                     /locus_tag="VFC15_19440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM12387.1"
                     /translation="MKIAIVGTRGIPNRYGGFERFAEQISSRFADQGHEVTVYCRRAF
                     VRPDDVYDRRVRRVIVPSLHQKHLDTWVSTFFAAVRVAFSDADVVLLCNVANSPFAYI
                     PRLFGKPVVLNVDGLDRKREKWKGLGAQVLHFCEWMSSFSSTRLVTDAKAIHDYYLTK
                     YGSESVVIGYGSEMPAGDDSLNGFNLESGRYVLYVSRLEPENNPDLVLRSWRNVRSDW
                     PLVMVGDNPYDHGYLERMKSLGDERVRFTGAIYGDGYWALQKHAGVFVFACEIGGVHP
                     ALIEAMAANNAVLYLDTPENAESAGDAGVKYEKSEEDLTAKLQSLLDDPAARQQLAAR
                     AKQRADALYRWDVIAKKYEKLFEELVTR"
     gene            1407..2594
                     /locus_tag="VFC15_19445"
     CDS             1407..2594
                     /locus_tag="VFC15_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12388.1"
                     /translation="MARRLLAPVAIQAKDNTVVSVNLGSFVRRILLFETYVLQSVWLN
                     DIQLLRQSFGDSGLIQLFKSGALKLYCESFTIGETGRVRADLRFAGNNKRLPLGSYSF
                     SMLRVQGQQQKIEKGLARFGTSLQDEAANSLLYIEPQGFTKQVFDGFYADLRGTSDIV
                     KRAVEIELRSLGIKPKQLRVEIEEIDSEDFKAVHNLQSQYGLSEAKSHDVILKSLLAI
                     GGLNQRFAEMIVHSALSGITDNDLPLLQGKLKLVAGLAGDVIRSEKRLERVVGLAGLP
                     DPVFGETRVDVEKLLRLRDSDECRAFRDWLAQSDSLSDKEIRERINSLSSKIKLFLNT
                     VPGKLTRFLVSGGLPLTPGVGLVLGMGASIVDAFILDKLAPKDGIVSFLSDSYPSVFK
                     RPR"
     gene            complement(2625..3152)
                     /locus_tag="VFC15_19450"
     CDS             complement(2625..3152)
                     /locus_tag="VFC15_19450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM12389.1"
                     /translation="MARQPGVYATLDTSEGTIVCRLFEEEAPITVKNFIELAEGSREW
                     THPHTKNKSKARLYDGTIFHRVIPDFMVQGGDPAGTGMGGPGYQFQDETKGSPHKFDK
                     PGKLAMANSGPGTNGSQFFITVAATQWLTGNHTIFGEVVEGQDVVDKITKLPRNRQDK
                     PNKDVVLNSVKIERV"
BASE COUNT          694 a          854 c          953 g          724 t
ORIGIN      
        1 ggagagcgtc atgatcacaa ccccgccctg aagggcgggg ctaccatctt tcgcgcccga
       61 ggcgctcgtg gtctgccacg tccagcgccg gtctgacaat agctaaccgc taatggccaa
      121 aagccaactg cgaaaagcga agagcgaaga gcgaacagcg aaaagcggcc tcatgaaaat
      181 tgccatcgtc ggtacccgcg gcattcccaa ccgctatggc ggcttcgagc gctttgccga
      241 gcagatcagc tcacgctttg cggatcaggg acacgaggtc accgtgtact gccgcagggc
      301 cttcgtccgg ccggatgatg tgtacgaccg gcgcgtgcgc cgggtgatcg tccccagcct
      361 tcatcaaaag catctcgaca cctgggtcag cacatttttc gcagccgtgc gcgtggcctt
      421 cagcgacgcc gatgttgtgc tgctgtgcaa cgtggccaac agtccgttcg cctacattcc
      481 gcggctcttt ggcaagccgg tagtgctcaa cgtggacggc ctcgaccgca agcgcgagaa
      541 gtggaagggc ctgggcgcgc aggtgctgca cttctgcgaa tggatgtcgt cgtttagttc
      601 gactcgcctg gtgacggacg cgaaggcgat tcacgattat tacctcacga agtacggtag
      661 cgagtcggtt gtaatcggct acggctccga gatgccggcg ggcgacgaca gcctgaatgg
      721 cttcaatctc gagtcgggac gctacgttct ctacgtcagc cgtcttgagc cagagaataa
      781 tcccgatctc gtgttgcgta gttggcgcaa cgtgcgcagc gactggcctc tggtcatggt
      841 gggcgacaat ccttacgatc acgggtacct cgaacggatg aaatccttag gcgacgaacg
      901 cgtgcgtttc accggcgcga tctatggcga tggatattgg gcgttgcaga agcacgctgg
      961 cgtcttcgtc ttcgcttgcg agatcggcgg agtccatcca gcgctgatcg aagcgatggc
     1021 ggcgaacaat gcggtgctct atctcgatac cccggagaac gccgaaagcg cgggtgatgc
     1081 gggggtgaag tatgagaagt cggaagagga ccttacagcg aagttgcagt cgttgctcga
     1141 cgatcctgcc gctagacagc agctagccgc acgcgccaag cagcgggccg atgcgctgta
     1201 tcgctgggat gtgattgcaa agaagtacga gaagctgttt gaggagctgg tgacgagata
     1261 gctgtcagcc atcagccatc agctttcagc caaacgaatt ttcggtgtca tcgtgagccg
     1321 agtgatatca cgaggcggat cgaacagaga agagaattcg attctttcca atcgaagaac
     1381 gccgggtctt agtcaattcc atgtcaatgg cacgcagatt gttagcccca gtcgcaattc
     1441 aagcgaaaga taataccgta gtgtcggtta acttaggttc gtttgttcgc agaattctgc
     1501 tgtttgagac gtatgttctc caatccgtat ggctgaatga catacaactc ctgaggcaat
     1561 cgttcggcga ctcgggtctc attcaactgt ttaaaagcgg tgcgttgaag ctgtactgcg
     1621 agagctttac gatcggcgaa accggccggg tgcgggccga cctgcgtttt gccggtaata
     1681 acaagcgact tccactaggt tcctattcgt tttcgatgct tcgcgtccaa ggccaacaac
     1741 agaaaattga gaaggggctc gctcgtttcg gaacctctct gcaagatgag gccgcaaaca
     1801 gccttctcta catcgaaccg caggggttta caaagcaggt ttttgacggt ttctatgccg
     1861 atcttcgtgg aacatctgac attgtcaaac gtgcagtgga gatcgagctg cggagtttgg
     1921 gaataaagcc caagcaactc agggtggaga tcgaggaaat tgactccgaa gatttcaaag
     1981 cagtacataa tctccagtca caatacgggc tttcagaggc gaaatcccat gatgtcatct
     2041 tgaaaagcct ccttgcgatc ggcggattga atcaaagatt tgctgagatg atagtccact
     2101 ccgcgctttc tggaatcaca gacaacgacc ttccgctgct ccaaggcaag ttgaagttgg
     2161 tcgccggttt ggcaggagac gtaatccgtt ctgagaaacg ccttgaaaga gtggtgggtc
     2221 tcgcaggtct ccccgatccc gtattcggag agacaagggt tgacgtcgag aaattgctga
     2281 gattgcgtga ctcggatgaa tgtcgagcgt ttagggattg gttggcacaa agcgattcac
     2341 tatcggacaa agaaatccga gagcgaatca atagcctgtc atccaagatc aagcttttcc
     2401 taaatacggt tcccggaaag ctcacgcgtt ttctcgtatc cggtgggctc ccgttaaccc
     2461 ccggggtcgg cctggtgttg ggtatggggg caagtatcgt ggacgcgttt atcctcgata
     2521 aactcgctcc gaaggatggg attgtgagct ttctcagcga cagctaccca tcggtcttca
     2581 aacgtccgcg ataaaaccgt ccactacgtc aaatacctgc tgcctcacac ccgctcgatc
     2641 tttaccgaat tcagcaccac gtccttgttg ggcttgtcct ggcgattgcg cgggagcttg
     2701 gtgatcttgt ccaccacgtc ctggccctcg acgacttcgc cgaagatggt gtgattaccc
     2761 gttagccatt gggtggccgc gaccgtgatg aagaactgcg agccgttggt gccggggccg
     2821 gagttggcca tggctagctt gcccggcttg tcgaacttgt gcggggagcc cttggtctcg
     2881 tcctggaact ggtagccggg gccacccatg cccgtgccgg cggggtctcc gccctggacc
     2941 atgaagtcgg gaatcacccg atggaagata gtgccgtcat acagacgagc tttgctcttg
     3001 ttcttggtgt gcgggtgggt ccattcgcgc gagccttccg caagctcaat gaagttcttc
     3061 accgtgatgg gcgcttcctc ttcaaagagg cggcagacaa tggttccttc gctggtgtcc
     3121 agggttgcat aaacgccggg ttgccgggcc atgatgattc ctttcgtcga tggctgaagt
     3181 tatgacggct cctgtaggga gccggtacag ctcgggcgag actgc
//
LOCUS       DASRKK010000811         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_970456, whole genome shotgun sequence.
ACCESSION   DASRKK010000811 DASRKK010000000
VERSION     DASRKK010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970456"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFC15_19455"
     CDS             <1..990
                     /locus_tag="VFC15_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZM12390.1"
                     /translation="TIAIFLGLAAINISKAGVGVQLPPSTTAESVPVTKLSAQDTILH
                     IFPENIAKAVADGAVLEVVVFSLIFAVALAMIGEQKRRPMLAFCESLSEVMFKFTNIV
                     MYMAPLGVGAAVAYTVGHTGLGILASLLKLLLTLYVALAVFVLGVLLPVALIFRVPLR
                     RFLKAVAEPVTIAFGTASSEAALPRAMEAMEAIGVPRQIVAFVIPTGYSFNLDGSSLY
                     LSVAAIFVAQVSGIHLSLGQQLLIMLTLMVTSKGVAGVSRAALVILLATASSTGLPTE
                     PVFLLLGVDQLLDMGRTAVNVLGNCLASVVMAKCEGEFGVETPSASVKEAVLQ"
     gene            complement(1240..2007)
                     /locus_tag="VFC15_19460"
     CDS             complement(1240..2007)
                     /locus_tag="VFC15_19460"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HZM12391.1"
                     /translation="MLAQERRESFTTRALRSGHALILEYDNMTDPTRKSWMRRGVEGA
                     VRSAFMKAYSTVKVNPEKYLDHLRMAYNLPASTYEGMYSVDPGTLDHIAEQTIRAHAR
                     LATVEGAGLGLGGMFTMLPDLGILAAITLRMIQKLSLIYGFPYNTEEEEAELWVAAAS
                     AAGVDISRELLEKQFVSKFVPRVIQRIAVSASAEIVEKWTARLIPLVSSAIGAGLNHY
                     FVKVWGERAMGHFRQKHLQMRSQQVNSKVRPPALPAS"
BASE COUNT          354 a          617 c          635 g          459 t
ORIGIN      
        1 acgatcgcga tcttcctcgg gctggcagcc atcaacatca gcaaagccgg agtcggcgtc
       61 caattgccac cttccacaac cgccgagtcg gtgccagtca cgaagctcag cgcgcaagac
      121 accatcctgc acatctttcc ggagaacatt gcgaaggcag tcgctgacgg ggcagtgctg
      181 gaggtcgtcg tcttcagcct gatcttcgcc gttgccctcg ccatgatcgg cgagcaaaag
      241 cgccgtccca tgctggcgtt ctgcgagagc ctgagcgaag tgatgttcaa gttcacgaac
      301 atcgttatgt atatggctcc gctcggtgtc ggtgcggccg ttgcctacac tgtgggacac
      361 accgggctgg gcatcctggc cagcttgctg aagttgcttc tcacgctgta cgtcgcgctc
      421 gcggtttttg tgctcggcgt gttgctgccc gttgcgctga tcttccgggt tccgctgcgc
      481 cgcttcctga aggcggtggc cgagccagta acgatcgcat tcggcaccgc cagttccgag
      541 gccgctctgc cgcgagccat ggaggcgatg gaagccatcg gcgttccgcg gcagattgtc
      601 gcgttcgtca tccccacggg ctacagcttc aatctggacg gcagctcgtt gtatctgtcg
      661 gtcgcggcga tttttgtggc ccaggtctcg ggcattcatc tttccctggg gcagcaactg
      721 ttgatcatgt tgacgctgat ggtcacgagc aaaggagtcg cgggtgtctc gcgagcggcg
      781 ctggtcatcc tgctggcgac cgccagctcc actggactgc ctaccgaacc ggtatttctg
      841 ctgctcggcg tcgatcagtt gctcgatatg ggccgtaccg cagtcaacgt gctgggcaac
      901 tgcctggcgt cggtggtcat ggcaaagtgt gagggcgaat tcggcgtaga gacgccatcg
      961 gcgtccgtga aggaagcggt gttgcagtga ggcgcggtgc ggcgaagatt agatcagatt
     1021 ctgcgtttgg tgtgtccggg tggagcaccg cttcagcggt gcatttgagc ggggtctttg
     1081 aatttggctt tagccgctga ggtgacttgg gcccttacct cagcggctaa agcctcgcct
     1141 ttttctgtac ccttggtatg cacgcctgaa ggcgtgctcc acccaacacc actggataca
     1201 ggaactctgg aatcgctcta gcagggtcgc tggctggcgt taactcgcgg gaagagccgg
     1261 gggcctgact ttgctgttga cctgctgcga ccgcatttgc agatgcttct ggcggaagtg
     1321 tcccatggcg cgctcgcccc agaccttcac aaagtaatgg ttcagccccg ctccaatcgc
     1381 ggagctgacc agcggaatga gacgcgctgt ccacttctcc acgatttccg cgctggcgct
     1441 caccgcgatc cgctgaatga cccggggaac aaacttagac acgaactgct tttccagcag
     1501 ttcgcggctg atgtccacac ccgccgcgct ggccgccgca acccacagtt cagcctcttc
     1561 ttcttcggtg ttgtagggga agccgtaaat caggctaagc ttctggatca tgcgcagggt
     1621 gatcgcggcc aggatgccga gatcgggcag catggtgaac atgccgccca gacccaggcc
     1681 cgcgccttcc acggtggcca ggcgcgcgtg agcgcggatg gtctgttccg caatatggtc
     1741 cagcgtgccg ggatccacgg aatacatgcc ctcgtaggtc gaggcaggca ggttgtaagc
     1801 catgcgcaga tggtcgagat acttctcggg attaactttg acggtggagt aggctttcat
     1861 gaaggcgcta cgcacggcgc cttccacgcc ccgcctcatc cacgattttc ttgtcgggtc
     1921 tgtcatgttg tcgtactcaa gtataagcgc gtgcccgctt cgcagcgctc gcgtcgtgaa
     1981 actttcccgg cgctcctgtg ctagcatcga cacttgcaca tgtctatggg aaagctacaa
     2041 gttgtgcccc tcgggggact cggcg
//
LOCUS       DASRKK010000812         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_285498, whole genome shotgun sequence.
ACCESSION   DASRKK010000812 DASRKK010000000
VERSION     DASRKK010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285498"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..2211
                     /locus_tag="VFC15_19465"
     CDS             496..2211
                     /locus_tag="VFC15_19465"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZM12392.1"
                     /translation="MASLVAKKKGNQLYYYVVESARVDGQPRIVHQSYLGSAEKVAAL
                     VRDRTAPVPLAATMRDFGLSGALWLAAQKTGVFDLLQQLWPQPRSGPSAAHYLLLAAI
                     HRICQPGPKTEVGDWYDRTVLQPLWGFSPERFTSQAFWDCFEALAPEHPAAGTSDQLE
                     QAQLRLVGLWKQKQLVSRRLLGYDTTNFYTYIASTNTQNHLAQRGHNKQGRHNLRQVG
                     LSYVLDGEHGLSLCHHVYAGDTPDVEEFSVALARMLGLLDQNQIARDTVTLVFDKGSA
                     ALANTVELDQAQVGWIAALPWNQAPAELRERPLEQLLTCNSDQPGVHAAGEKMLVHGK
                     EYLCAVKYSASFAGEQLHSVTTGVSKVLQYLRRLSVDLSKPKGRWTEAQIRNKIQRWL
                     SAQFMAELIHYQLELRDGHWHLQFDFDHAAWLHLMAHRLGRTVLLTNRMDWSAEQVAA
                     AYSGQQHVEQVFRGLKDGEWLGWGPMHHWTDSKIRVHAFYCMLGISLLQYVHRQAQAA
                     WAGISMEKLLEELRQIQEFVLLYPPQGDKGPNRVATVLSKQTLTQQSLAKTLGLEQLR
                     ITPRG"
     gene            complement(2238..2390)
                     /locus_tag="VFC15_19470"
     CDS             complement(2238..2390)
                     /locus_tag="VFC15_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HZM12393.1"
                     /translation="MFFPDSVVVGDVNGDHKLDIVTVSGLAKTVAVLLAGVYWERPEQ
                     ERKTLF"
BASE COUNT          508 a          743 c          743 g          517 t
ORIGIN      
        1 caagagcaac gcggccggct gcggccttgc gtttcttcgc gcggagaata ttccacctgg
       61 ggaggcgccg gaaggtgggt caggtcatca cggttaggcg gatcgtgcga gcgcggccgg
      121 aggagagctt gagtgcggga tgcaccgcgg caggagccag gggctgccac cctggcgcct
      181 ggcgggtttt gacggtgcgg caccagtcgg acacacccgg cgcgggcttg agtatattgg
      241 cctcatcgac gccgctgacg tacatatcgg ggagcgtggg catccgtacg gcttccgcag
      301 gccctagcgg gagtttactg ccaggatcaa ggttcgcagc ccgtcccggc gggtaaccaa
      361 ttgagaaatc gcgatttgcc cgcggcctga tgtttcgaat gccgtattac cctatttatg
      421 atctgtttat agcgatttct gtaccatggc tattgaacct ccggagccca ttagcgtata
      481 ctcgtattac cctatatggc cagcctcgtt gcaaagaaga aaggcaacca gctctactac
      541 tacgtcgtcg agagtgcccg cgttgacggt cagcctcgca tcgtccacca gagctacctc
      601 ggctccgccg aaaaagtggc tgccttggtc cgcgaccgca ccgccccggt gcctctggcc
      661 gccaccatgc gcgacttcgg cctgtcgggt gctctgtggt tggctgccca gaaaaccggt
      721 gtcttcgact tgctgcagca actctggccg cagccccgct cgggaccctc ggccgcgcat
      781 tacctgctct tggccgccat tcaccgcatc tgtcagccgg gcccgaaaac cgaggtgggc
      841 gactggtatg accgcaccgt gctgcaaccg ctatggggct tttccccgga acgattcacc
      901 tcccaagcct tctgggattg tttcgaggct ttggctccag agcacccggc cgcgggaacc
      961 agcgaccaac tagaacaggc gcaactgcgc ttggtcggtt tatggaagca gaagcagttg
     1021 gtcagccgcc gcttgctggg ctacgacacc accaactttt atacctacat cgccagcacc
     1081 aacacgcaga accatctggc acagcgcggt cacaataagc agggccgcca caacttgcgc
     1141 caggtgggtc tcagctatgt gttagatggg gagcatggcc tgagcctgtg tcaccacgtc
     1201 tatgctggcg acaccccaga cgtagaggag ttctcggtag ctttggcccg catgttgggc
     1261 ctgttggatc agaatcagat tgcgcgggac acggtcactt tggtgttcga caaaggctcg
     1321 gcggccttgg ccaataccgt tgagttggac caggcgcagg tgggatggat cgccgcgctg
     1381 ccttggaatc aggcgcccgc agagttgcgt gagcggccct tagaacagct cctcacctgt
     1441 aacagcgatc agcccggagt gcatgccgct ggcgaaaaga tgctggtgca cggcaaagaa
     1501 tatctctgcg cagtgaagta ctcggcctct tttgcgggtg aacaactgca cagtgtgacg
     1561 accggcgtga gcaaggtcct gcaatatctg cggcgcctgt ccgtagatct gagtaaaccc
     1621 aaggggcgtt ggaccgaagc ccagattcgc aataagatcc agcgttggtt gtccgcgcaa
     1681 ttcatggccg agttaatcca ctaccaactg gaattgagag acggacactg gcatctgcaa
     1741 ttcgattttg atcacgccgc ttggttgcac ctgatggctc accgtctggg ccgaaccgtg
     1801 ttgctcacca atcgcatgga ttggagtgcc gagcaggtcg ctgccgccta ctctggccaa
     1861 caacacgtag agcaggtctt tcgcggactc aaagatgggg agtggctggg ctgggggccc
     1921 atgcatcatt ggaccgacag taagatccgg gtgcacgcat tctattgcat gctgggaatc
     1981 tccctattgc aatacgtgca ccggcaggcc caggcggcat gggccggcat ttcgatggag
     2041 aagctgctgg aggagttgcg tcagatccag gaatttgtcc tgctctaccc gccacagggc
     2101 gacaagggac cgaaccgagt cgccactgtc ctttccaaac agacgctcac gcagcagtcg
     2161 ctggccaaaa cgctgggcct ggagcaactt cgtattaccc cgcgtgggta ataccgcaca
     2221 ccgctcgcaa cctcttatta aaacaaggtc ttacgctctt gttctggtcg ctcccagtaa
     2281 actcccgcta gcagcacggc gaccgttttg gcgagaccgc tgacggtcac gatatcgagc
     2341 ttgtggtcgc cattgacgtc gcccactacc accgaatcgg gaaagaacac gagagcgccc
     2401 agcagttaat ccaatggttc cctcagcaac tgctcgataa tggcgactgc gtctttcggc
     2461 ttgaacggct tctgtaatat tttacaacta gcgggagttt actgggagcg a
//
LOCUS       DASRKK010000813         6662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_92785, whole genome shotgun sequence.
ACCESSION   DASRKK010000813 DASRKK010000000
VERSION     DASRKK010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92785"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VFC15_19475"
     CDS             complement(<1..1661)
                     /locus_tag="VFC15_19475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM12394.1"
                     /translation="MAVPKPALDSDAMTSMGVPVPDVTIAEPAPPNPPKPVSPPEPAK
                     RDSSNDATLVPTTKKRDASSGTPSPIYAAIGATILEIGTVLGGRYEIQKLLGMGGMGA
                     VYKAHDLEVDRTLGLKVIRPDLAGNPAILARFKQELVLARQVTHRNIIRIYDLNEADG
                     VKFITMEYIEGEDLRTILTRAGKLPPKEAINIMRQACSGLLAAHAEGVIHRDLKPSNI
                     MRDGSGRVVIMDFGLAKTVQSDGMTQTGMMIGTMEYMSPEQAMGSELDARSDLFTMGL
                     IFYELLTGNIPFRAESAIASLVKRTQERATPLSDVDPTIPPTLSLIAAKCLERDPKAR
                     YNNVQELIDDLDIFEGKRSRSSSAGRTALGGSVYQAPAIEPSIPTPTQVPEKRFPLKW
                     IAIGVAGIVLAAGVGVVVKSKMSGGGGAQVSQAAALSLAIVPFYNASVDPTLNWLASS
                     LSETLSTDIGQSQRVRLVSPSRLQQVLRDLHISPQSQLDLSTLKRIAEFTNADTLVYG
                     QYQKFGDQIRINATVYDLKHDRNFELKTDVPSEKDLLGGLDNLAK"
     gene            complement(1946..3223)
                     /locus_tag="VFC15_19480"
     CDS             complement(1946..3223)
                     /locus_tag="VFC15_19480"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12395.1"
                     /translation="MASSGVSLSNYLRLVRGNANFRRLWIAQIVSENGDWFYTLAIYS
                     LLLELTGKASSVALALVLQVLPHTLLGPLAGVINDRFSRRKIMIVTDLLRMAIVACML
                     MVRSRGSVWLIYPLLFLETVMVAFFEPARNAVIPNIVAEGDVIVANTLSSATWSFDLA
                     VGSVLGGLVAAWLGREAVFVLNAVSFVVSAWLISRMHFVESHADEANRHFEDQGAGWS
                     SVRAGFHYIARDPKLAAVVSLKAGVSILGASWVLLPVMGERTFPVAIAGIPQARDALF
                     GMSLLMGARGVGALLGPLVASHWTGQRERSLRIAVLFGFIFGGIGYLLLGTANGVWFA
                     FAVIALAHSGTSTVWVFSTTLLHLNTKDRFLGRVFGADLAISMFVLAAASWIAGVALD
                     AGTAPRAVAVGTGAAMFLPAALWAFALRLWRPE"
     gene            complement(3353..4846)
                     /locus_tag="VFC15_19485"
     CDS             complement(3353..4846)
                     /locus_tag="VFC15_19485"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZM12396.1"
                     /translation="MRILLGLGRWLAFSYLLWCANLALGCDQLPEGQSLWIRLASPVS
                     TYTARVGDSVHAVLTQDVDCGSDVVLPMGTSIEGVVRSKRKVGWGIRHETSALEIEFN
                     RVTEANGSAIDISARVEEVENAREQVKHGVIQGVLSSDTFQGRVNSRLIHLPTWNPYS
                     DLGLIIYKATFPIFPEPEIYYPAGTDLRLKVKAAVSSLPIAVKTTLEPAPADTSQLDA
                     WIQQMPQRSTTAKSVNADLLNLVFLGSREQVQAAFREAGWHNSDPVSRHSFANNLYAL
                     LNNSGYAQQPMTTFLLDGKPEDMNWQKGLNSYGRRDHLRIWRWTGEESADSVWVSSST
                     RDTSAILSVKYHGFVHHISPNIDDERSKVIRDLNFAGCVSSVSYVSRPGISTITQNAT
                     GDAVRTDGSIAVVQLRDCEPANPELGGNSTTAKFKPGNHAFRYMRREILTFRNDIWRA
                     NVIYGLYDVGRMAITAMRHQPVPPLDDESRPTIAAAAQVGVAGLGIQ"
     gene            complement(5095..6525)
                     /locus_tag="VFC15_19490"
     CDS             complement(5095..6525)
                     /locus_tag="VFC15_19490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZM12397.1"
                     /translation="MSNELRNFLALPYDELEELNLQAKDQRLKRVATHKIQEERLKYL
                     TDEKRIKAVTLLFSDLEGRLHMLDYDKKFLIKSWDNMTFDGSSIRGFTAQRESDLRLG
                     IDWSAFYWGPADVFGPGKILVFGEVIDKGGSPYGADIRGVLKGFAADLYKNEGYTLNA
                     ANEIEGFLFAGKDAERHYDETGKFEYVNTGGYYHSLPGDTLRTFIDTTAEVQRAMGFQ
                     NEKDHPEVAPSQFEINYGYGEVSAAADQIQLYKLICRQVATNMGLTASFLPKPVVGVN
                     GSGMHTNVSINKGGKNLFWDPKGEEKLSKMGWQFVDRFLTHGNDTCLVFNSSVNAYRR
                     LDPHFEAPNQIKASAVDRGSMIRIPIGNEKSMRVEVRSVAPDANPYLVMLTVFKTGLF
                     GETAKIKNLRQAERYLPDNIYDALDNFRKSEWTTKLLGADVKGRFADLKQASADRCAR
                     LLGTFVKAPEVQYHHDVYNQYLWNKF"
BASE COUNT         1460 a         1937 c         1864 g         1401 t
ORIGIN      
        1 tgcttggcca gattgtccaa tccgccgagc aagtccttct cgctcggaac gtctgtcttg
       61 agctcaaaat tgcgatcgtg cttcaagtcg taaacggtcg cattgatgcg tatctgatcg
      121 ccgaacttct ggtactggcc atacaccagt gtgtcagcgt tggtaaattc agcgatgcgc
      181 ttcaacgtcg ataggtcaag ttgcgattgc ggcgaaatgt gcagatcgcg caggacctgt
      241 tgcagtcggc tgggtgaaac caatcgcacg cgctgcgact ggccaatatc ggtgctcaaa
      301 gtctccgaga ggctggatgc caaccagttc aaggttgggt ccaccgatgc gttatagaac
      361 gggacgatcg ccagcgagag cgccgcggct tgcgaaacct gcgccccacc accgccgctc
      421 atttttgatt tgacaactac accgactccc gctgccagca caataccggc aacgccaatc
      481 gcaatccact tgagggggaa gcgtttctcc ggcacctgag taggtgtcgg tatcgacggc
      541 tcgatcgctg gggcctggta cacagacccg cccaatgcgg tgcgccctgc actgctggat
      601 cgcgagcgct tgccctcgaa gatgtcgagg tcgtcgatca gttcctgaac gttgttgtag
      661 cgtgctttcg ggtcgcgctc caggcacttg gccgcgatca ggctcaacgt cggcggaatc
      721 gtcgggtcga cgtcagagag cggcgtagct cgctcctggg tacgcttcac caggctggca
      781 atggcgctct cggcgcggaa cgggatgttt cccgtcagaa gttcatagaa aatcagcccc
      841 atcgtgaaca ggtcggagcg tgcatccaac tccgagccca tcgcctgctc aggcgacata
      901 tactccatcg tcccgatcat catgccggtc tgcgtcatgc cgtcgctttg gacggttttc
      961 gccaacccga agtccatgat gactacgcgg cccgacccgt cgcgcatgat gttactgggc
     1021 ttcaaatcgc gatgtatgac gccttcggcg tgcgcggcca gcaagcccga gcacgcctgc
     1081 cgcatgatgt tgatggcttc cttcggcggg agcttgcctg cccgtgtcag gatggtgcgc
     1141 aaatcttcac cttcgatgta ttccatcgtg atgaacttca cgccgtcggc ttcgttcaga
     1201 tcgtaaatgc ggatgatgtt gcgatgggtg acctgtcgcg ccagcaccaa ttcctgcttg
     1261 aatcgggcga ggattgcggg attgccagca agatcgggcc gaatgacctt gagccccaac
     1321 gtccggtcaa cctcaagatc gtgcgccttg tacaccgcgc ccataccgcc catgccgagc
     1381 agcttctgga tttcgtaccg cccgccgagg acagtaccga tctccaaaat ggtagcgccg
     1441 atggcggcat aaatcggact gggtgtaccg gagctggcat cgcgcttctt cgtcgtgggc
     1501 acgagggtgg catcgttgga cgaatcgcgc ttggcgggtt ctggaggcga cactggcttt
     1561 ggtggatttg gaggagcggg ctccgcgatg gtgacgtccg gaacaggaac tcccatggaa
     1621 gtcatcgcat cggaatcgag ggcaggcttc gggacagcca tcgaactgtc gccgctggaa
     1681 acagcagttc cctcctgcgc tctccgagat gctcggtccg cgtcgtctgg catgttcccc
     1741 acagtgtgtt tggcctgtct gatctggcgg aaatcccgcc gcagagtcag ctatctccgc
     1801 agtatatctt gctttcggaa atacagattg cggattctac gtatgtcatc agtagttaac
     1861 aggcccactg ccgacgttat cactgcagac aacacggagg ttgactgtca tatagcactt
     1921 aagtctcaaa tttgcggcca tatcgtcact ccggcctcca tagacgcagg gcgaaggccc
     1981 acagcgcggc gggcaaaaac atggccgcac ccgttccaac cgccaccgcc cggggggcgg
     2041 ttccagcgtc aagcgccacc ccggcaatcc atgacgcggc cgccaataca aacattgaaa
     2101 ttgcgaggtc cgcgccgaag actctcccca gaaagcggtc tttggtgttc aagtgcagca
     2161 gggtggtgga gaacacccat accgtcgaag tgccggagtg cgccagtgcg atcacagcaa
     2221 aggcaaacca gactccgtta gcagtgccga gcaagagata tccaataccg ccgaagatga
     2281 acccgaacaa tactgcaatg cgcaaactgc gttcccgctg acccgtccaa tgcgacgcaa
     2341 cgagaggtcc cagaagcgct cccacgccac gggctcccat caataagctc attccgaaca
     2401 acgcatcgcg tgcctgcgga atacctgcga tcgcaaccgg gaaagtgcgc tcccccatca
     2461 caggcagcag cacccaagac gcgccgagaa tactgacgcc cgccttgagt gaaaccacgg
     2521 ccgccagttt aggatcgcga gcgatgtagt ggaatcctgc gcggaccgaa gaccaccctg
     2581 caccttggtc ttcaaaatgc cggttggctt cgtcggcatg cgactcgacg aaatgcatgc
     2641 ggctgatcaa ccaagctgag actacaaagg acaccgcgtt cagcacgaag accgcttcgc
     2701 gtccaagcca cgcagcgacg agacctccca gtactgaccc tactgctaaa tcaaacgacc
     2761 atgtcgccga ggaaagcgtg ttggcaacga tgacgtcacc ttccgcaacg atattcggaa
     2821 tgaccgcgtt gcgggccggt tcgaaaaacg ccaccatcac ggtttcaaga aacaggagcg
     2881 gataaatcag ccataccgag ccgcgcgagc gcaccatcag catgcaagcc acaatcgcca
     2941 tccgtagaag gtcggtgacg atcatgattt tccgtcgact gaagcggtcg ttgatcacgc
     3001 ccgcaagtgg ccccagcaac gtgtgcggca gcacttgcaa taccaaggcc agtgcgaccg
     3061 aggacgcttt acccgtcaac tccagaagca agctgtaaat ggcaagggtg taaaaccagt
     3121 ctccgttttc gctgacgatc tgggcaatcc agagcctgcg gaaattggca ttaccgcgga
     3181 ccaggcgaag gtagttggag aggctgacgc cagaggatgc catgcgaaac catcagccta
     3241 tcaaaaggtg ggtattcgcc cgtggggctc gagcagaatt ttacgggacg ttgtggcgca
     3301 tagttaacag ttagcgtcgc ggcccgatag cttagagatc agcagacgga gtttactgga
     3361 tacccaatcc ggccactccg acttgcgctg ctgcggcgat cgttggtctg gattcatcgt
     3421 ccagtggagg cactggctga tggcgcattg ctgtgattgc catccgtcca acgtcataca
     3481 gaccgtagat gacgttggcc cgccagatat cgtttcggaa ggtgagaatc tcacggcgca
     3541 tataacggaa agcgtgattc ccgggcttga atttcgcagt cgtcgaattg ccgccgagct
     3601 cgggattggc gggttcgcag tccctcaact ggacgacagc aatcgaaccg tcagtgcgaa
     3661 cggcgtcgcc tgtcgcgttc tgcgtgatcg tggagatccc aggccgggaa acatagctga
     3721 cagaacttac gcacccggca aaattaaggt cgcgaatcac tttgctgcgc tcgtcgtcaa
     3781 tattgggaga aatgtggtga acgaaaccgt ggtacttcac cgagagaatg gcgctggtat
     3841 cgcgcgtgga ggaacttacc cacaccgagt cagctgactc ttctcccgtc caccgccata
     3901 tgcgcagatg gtcccgccgg ccgtaactgt tcaggccttt ctgccagttc atgtcttccg
     3961 gtttcccgtc cagcaaaaaa gtcgtcatcg gttgttgcgc ataaccgcta ttgttcaaca
     4021 aggcgtaaag attgtttgcg aatgagtgcc tcgacaccgg atcgctgttg tgccaccctg
     4081 cctcccgaaa tgccgcctgc acttgctcgc gagatcccag aaacaccagg ttcaacaagt
     4141 cagcattcac ggacttggcg gtcgtcgaac gctgcggcat ctgctgaatc cacgcatcga
     4201 gttgcgatgt atcggccgga gcaggttcaa gcgttgtctt gactgcgatc ggaagtgatg
     4261 agaccgccgc cttcaccttc aggcgcaagt cggtaccagc tggataataa atttccggct
     4321 cggggaaaat cggaaaggtt gccttgtaga tgatcaatcc cagatcggag taaggattcc
     4381 atgtcggcag gtgaatcagg cggctgttga ctctgccttg aaacgtatcg ctgctgagca
     4441 cgccttggat gacaccgtgc tttacctgct cgcgggcatt ttccacttcc tcgacgcggg
     4501 cggagatatc gatggcggag ccatttgcct cagtgacacg attgaattcg atttcgaggg
     4561 ccgaggtttc atgccggatg ccccatccca ctttgcgttt gctacgaacc acgccctcga
     4621 tggatgtgcc catgggcaac acaacgtcgc ttccacagtc aacgtcctgc gtcagaaccg
     4681 catgtacaga gtcgcccaca cgtgctgtgt aggtggaaac aggactggcc aagcgaatcc
     4741 agagcgactg gccttcaggc aattggtcgc aacccaacgc caaattagcg caccacaaca
     4801 aatatgagaa tgcgagccag cggcccagac cgagcaggat tctcatgaaa acgaagcgcc
     4861 ttccctcatc tgaattaggt gatgattgat gattaatgat aactgacaac cgccgcggga
     4921 cccaatcgtt gcaccgtaat cctgctggtt cctggggcct gaccaatatc gcatgacaga
     4981 gcgcattctg atgccgcagg gcgagtgcca cagtgaatta accaagctga aatgcagacg
     5041 ccccggacag cccggggcgt ctgcattctg cgatgtactg atacaaccga gactctagaa
     5101 cttgttccag aggtactggt tgtaaacgtc gtggtggtac tgcacctccg gtgccttaac
     5161 gaaggtcccc aacaggcgcg cacagcggtc ggcggaggcc tgcttcaggt cggcaaaacg
     5221 ccccttcaca tccgcaccga gaagttttgt cgtccactcc gatttacgga agttgtccag
     5281 cgcgtcatag atgttgtcgg gcagataccg ctcggcttga cgaaggttct tgatcttcgc
     5341 ggtttcgccg aacaagcctg tcttgaacac cgtaagcatg accaagtagg ggttcgcatc
     5401 gggggcaacc gaccgaactt ccacacgcat gctcttctca ttgccgatcg ggatacggat
     5461 catggaacca cgatcgactg ccgatgcctt gatctggttc ggcgcttcga agtgaggatc
     5521 gagacggcga tacgcgttaa cgctggaatt aaacaccaag caggtatcgt tgccatgagt
     5581 caagaaacgg tcgacgaact gccatcccat cttgctgagt ttttcctcgc ctttcgggtc
     5641 ccagaacagg ttcttgccac ccttgttgat ggacacgttg gtgtgcatgc cgctgccatt
     5701 gacgcccacg accggcttgg gcaggaacga cgccgtgagt cccatgttgg tggccacctg
     5761 gcgacagatc agcttgtaaa gctgaatctg gtcggctgcc gcggaaactt caccgtaacc
     5821 gtaattaatc tcaaattgcg aaggcgcgac ttccgggtgg tccttctcgt tctggaagcc
     5881 catcgcccgc tgcacttcgg ccgtcgtatc gatgaacgtg cgcagggtat cacccggcag
     5941 cgagtgataa tagccgccgg tgttgacgta ttcgaatttg ccggtttcgt cgtagtgccg
     6001 ctccgcgtct tttccggcga agaggaatcc ctcgatctcg ttcgcggcgt tcagggtgta
     6061 gccttcattc ttgtacaagt ccgctgcgaa ccccttcagt acgccacgga tgtcggcccc
     6121 gtaaggagat ccgcccttgt cgatgacctc gccgaagacc aggatctttc ccggcccaaa
     6181 cacgtcggcc ggcccccagt aaaaggcgct ccagtcgatg cccaggcgca aatcgctctc
     6241 gcgctgcgcc gtaaagccgc gaatcgaaga cccgtcgaag gtcatgttgt cccagctctt
     6301 gatcaggaat ttcttgtcat agtcgagcat gtgcagacgg ccttcgaggt cgctgaacag
     6361 cagagtcacc gccttgatac gcttttcgtc ggtgagatat ttcaggcgct cttcctgaat
     6421 cttgtgggtg gccacgcgct tcaggcgctg atccttggcc tgcagattca gctcctccag
     6481 ttcgtcatac ggaagtgcca agaagttacg caattcgttc gacatgaatc tcctcaggat
     6541 ggggattagg aaccgccgcg gtgaagttaa atttgctggc gatcactcat gagggtatag
     6601 gttgcccaat taacatcgcc aattaaaagc gttaatgagc gatattgacg gaatttatcg
     6661 aa
//
LOCUS       DASRKK010000814         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_385966, whole genome shotgun sequence.
ACCESSION   DASRKK010000814 DASRKK010000000
VERSION     DASRKK010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385966"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..920)
                     /locus_tag="VFC15_19495"
     CDS             complement(36..920)
                     /locus_tag="VFC15_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HZM12398.1"
                     /translation="MVKVEELLFRGHPEVVDADLADYFGSIPHAELLKSVARRIVDRR
                     VLHLIRMWLECPVEETDDRGRKTRTTEARDSRRGIPQGSPISPLLANLYMRRFVLGWK
                     MLGLEQSLGSRIVTYADDLVILCRRGKAEEALQQLRKIMSKLKLTVNEEKTQICKVPE
                     GEFDFLGYTFGRMYSARTGHARLGYRPSKKSIKRMVANVHELTVRSGTWQDTTKLVGA
                     LNRTLRGWANYFQVGTVNKAYRAVDSYTAVRLRRWLRAKYKVRRRKGGTYPLSHLYGH
                     FGLVRLTALGRDASWVKA"
     gene            complement(821..1354)
                     /locus_tag="VFC15_19500"
     CDS             complement(821..1354)
                     /locus_tag="VFC15_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12399.1"
                     /translation="MGNLSTPKSVQKLQTALHAKAKAEAGYRFYALYDKISREDILAH
                     AYAQCRSNKGAPGVDGQDFADIDAYGVQRWLGELALALRNETYRPDPIRRVFIPKANG
                     KLRPLGISTLRDRVCMTAAMLVLEPIFHPSSTLTVPGAMPNRRWSRWKSCCSVATRKS
                     LTLTSRTTSEASPMPSY"
     gene            2006..2872
                     /locus_tag="VFC15_19505"
     CDS             2006..2872
                     /locus_tag="VFC15_19505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM12400.1"
                     /translation="MRVQIIACLVLTILASFDPQATVSADELVKFDSAAYAVGKLQQR
                     LARERGEMPTSAPATTIEGYLSKPEGRGPFPAVVYLHGCSGLFESTRKQIAELITGWG
                     YVSFAVDSFATRGIKEACDQPMPDRQADALGALLYLSKLAFIDPERIAVLGSSQGGIA
                     ALQLASTHPVGLFAIPDELKFRDAVAYFPLCSVATDQLTIPTIILIGELDDWTPAKDC
                     ERWMERRGGKGAPVKLVIYPGAYHAFTAAAFRDGTRSYGHWLKYDADAARRSVLEVHD
                     FLATELIPVRRN"
BASE COUNT          597 a         1007 c          870 g          709 t
ORIGIN      
        1 tgcagatctc ccgcatccgg ctctcggaca agacttcacg ccttcaccca cgacgcgtcg
       61 cgcccaagcg cagtcaaacg tacgagcccg aagtgcccgt aaaggtgcga gagtggatag
      121 gtcccgccct tgcgtcgcct gaccttgtac ttggcgcgca accaccgacg caaccgcacc
      181 gcggtatagc tgtcgaccgc ccggtacgct ttgttgacgg tgcctacttg gaagtagttc
      241 gcccatccgc gcagcgtgcg attcaacgcg cctaccagct ttgtggtatc ttgccaggtc
      301 cccgatcgaa cggtcagctc atggacattc gctaccatgc gcttgatgct cttcttcgat
      361 ggccgatatc ccaagcgggc atgaccggtt ctcgcagaat acattcgccc gaacgtgtag
      421 cccagaaagt cgaactctcc ttccgggacc ttgcagattt gtgtcttctc ctcgttgacc
      481 gtgagcttca gcttgctcat aatcttgcgc agttgctgca acgcctcttc ggctttgcct
      541 ctccggcaca agatcacgag gtcgtcggcg taggtcacga tgcgagagcc gaggctttgc
      601 tccagcccga gcatcttcca tcccaacacg aaccggcgca tgtagagatt cgccagcaat
      661 ggtgagatgg gtgaaccttg cgggatgccg cgccggctgt cccgggcttc ggtcgtgcgt
      721 gtcttccttc ctcgatcgtc ggtttcttcc acggggcact ccagccacat cctgatcaga
      781 tgcagcacgc gccgatcaac aattcggcgc gccaccgatt ttagtagctc ggcatggggg
      841 atgcttccga agtagtccgc gaggtcagcg tcaacgactt ccgggtggcc acggaacagc
      901 agctcttcca ccttgaccac cgcctgttgg gcattgcgcc cgggacggta agcgtactgc
      961 tcgggtggaa gatcggctcc agcaccagca ttgctgctgt catgcagacc cgatcccgca
     1021 gggtcgagat gcccagcggc ctgagtttgc cgttggcctt cgggatgaac actcttctga
     1081 tggggtccgg tcggtaagtc tcgttcctga gcgcaagcgc cagttcgcca agccaccgct
     1141 gcaccccata cgcgtcgatg tccgcaaaat cctgaccgtc cacaccaggt gcgcccttgt
     1201 tggagcggca ctgggcatag gcgtgcgcca ggatgtcctc acggctgatc ttgtcataca
     1261 gggcgtagaa gcgatagccg gcttccgcct tcgctttcgc gtgcaacgcc gtctgcagct
     1321 tctgaacact tttcggagtt gataggttgc ccaatctcca ggtccctcac tacgtattgc
     1381 gttcgtcttg aactgaggtc ccttccctcc accggcatta cccggcttca gcggtactgc
     1441 gaacctctcc gcctccccag ggcgcccggc ctatccctca cgggcatccg gttggtcatc
     1501 cctgcccacg tcctggggtc tcccgtgttg cgtacgcttt ccttgtgtac atcctgacgc
     1561 cactaccccg gcgcagcggc tgggcctact cttcgctcat tcacccagcc gtatcagcct
     1621 tccccgaaag ggttgtcggg tcggcctgcg catcgtcctt ttcgaggctt gctcggcgtt
     1681 tactcacgtt gcggcctgca cactcgcgct gtcaccaatt cgtgacacgc taaccagagg
     1741 cttcagccat ttcgttacct ccatgactgc tccggttgct tccggctgga gccgttgccg
     1801 ggtgggactt gcacccactg gaaagcgccg ccttttcacg gcgcacaccc ggaccggaca
     1861 tcagagctga tttccctact cgaccttccg ccggactaag agacttgagc cttccttcga
     1921 ggcaagacag ctctcgataa cgaccagcga cagggaatag ggtattcgct gtaatctaag
     1981 tggcggcccg tcgggagatt gaaaagtgcg cgtccaaatt atcgcatgcc tcgtcctcac
     2041 aattctcgca tcgtttgatc cccaggccac cgtttcagca gacgaattgg tcaagttcga
     2101 cagcgctgct tacgccgttg gcaaactcca gcagcggctg gctcgcgagc gcggcgaaat
     2161 gccgacgagt gcgccagcta ccactattga aggctaccta tcgaaacctg agggaagggg
     2221 cccttttccg gccgttgtct atctccacgg atgcagcggt cttttcgaga gcacccgaaa
     2281 acagattgcc gaactcataa cagggtgggg ctatgtctcc tttgcagtgg acagctttgc
     2341 cacccgtggc atcaaagagg cctgtgacca gccaatgcct gatcggcaag cggatgcgtt
     2401 gggagcgttg ctctaccttt ccaaactcgc tttcatcgat ccagagcgga ttgcggttct
     2461 cgggtcgtcg cagggcggca tcgccgcgct ccagttggcc tcaactcacc cggtcgggct
     2521 ttttgcaatc cctgacgaat tgaagttcag ggacgccgtg gcctactttc ccttatgcag
     2581 cgttgccacc gatcagttga ccattccaac catcatcctg atcggcgagc ttgacgattg
     2641 gacgcctgcc aaagactgtg aacgctggat ggaaaggcgc gggggcaagg gggctcccgt
     2701 caagctggtc atctatcccg gtgcttatca tgcctttacc gcagccgcgt ttcgagatgg
     2761 cacgcgatcc tatggccatt ggctcaaata cgatgcggat gctgcgcggc ggtcggttct
     2821 ggaagtgcat gacttcctgg cgactgaact aattccagtt cggcggaact gacccctcca
     2881 gcctctttca tctgcggtgt tgcctattgg cacctttcaa acatgccaat gaacgccaag
     2941 aatgtctgtt gctcgggtag atggaagggc caagccgacg gccgaagtgg tgcggttgat
     3001 ccgaagcgga cattcgcgct ttggctccct tgatatgccg caatggcttc gccgcaaagc
     3061 cctgctatca aacgcactca gttcgcgcat tatcgcactg cggtcagaat ggatgcaaaa
     3121 atgacgatca atgacattgg cccaaagccg caatcgttca acattgaaca cgcgaccaag
     3181 gaa
//
LOCUS       DASRKK010000815         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_679169, whole genome shotgun sequence.
ACCESSION   DASRKK010000815 DASRKK010000000
VERSION     DASRKK010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679169"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..280)
                     /locus_tag="VFC15_19510"
     CDS             complement(137..280)
                     /locus_tag="VFC15_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12401.1"
                     /translation="MPRSSRLCEIVGLMPSDAMRSNLLRHWKGLTAFLRERVRAGLAH
                     ARR"
     gene            complement(672..1427)
                     /gene="istB"
                     /locus_tag="VFC15_19515"
     CDS             complement(672..1427)
                     /gene="istB"
                     /locus_tag="VFC15_19515"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM12402.1"
                     /translation="MLNQQTIEKLYTMRMRGMADAFTQQQEEPETTQLSFEERFALLV
                     DRQWSWRQNRVLERRLRDGRLQGPACMEDIDFRAARGLDKQVVRSLMNDSEWVRRHQH
                     IFLVGPTGIGKTFLAKAFGQKACRDGFTAYFATAAQLFRELELARADGSYAKRLRGLG
                     QVDVLIVDDWAMSPMAEGERRAFLEICDERYMTRSTLLTSQLPVAKWHAQIGDPTVAD
                     SILDRLVHAAHRIELQGESMRKKKGGRGGKDAE"
     gene            complement(1444..3000)
                     /gene="istA"
                     /locus_tag="VFC15_19520"
     CDS             complement(1444..3000)
                     /gene="istA"
                     /locus_tag="VFC15_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058167704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM12403.1"
                     /translation="MRKLKEVLRLHSLGLSQHQIARSCSISQSTVHEYLSAAQAAGVK
                     WPALEHWDDQQIERTLFPQRPAPAIWRKHPEPDWTQLHQELQTHKDLTLQLVWQEGRE
                     SNPDGYGYSRFCDLYRRWLKKLDLVLRQEHRAGEKMFVDYAGATIPVHNPENGDAHPA
                     AVFVAVLGASSYTFAEATTGQDLRNWIGSHMRAWEFFGGVAEVVVPDNLKSAVTHPSY
                     YEPDLNPTYRDLGEHYGVAIIPARPYRPREKAKAEVGVQVVQRWIVAALRKRKFFSLA
                     EVNQAIAELLVRLNQRPFRKREGSRATLFAQLDQPALKPLPATRYQFGEWEIARVNID
                     YHIEVERHYYSVPYALVHQQVDVHLTGETVEIIHRGVRVASHVRSYLREKATTLTEHM
                     PKAHQKYVGRTPSRLIEDAQQTGPYTGQLVEAILAAKRHPEQGYRSCLGILRLAKTYP
                     AERMEAAARRCLRARAYNCQSMDSILKNQLDRLPLPGDPPVQPAVNHENIRGADYFDS
                     PPEAEPPVIQ"
BASE COUNT          603 a         1059 c         1000 g          728 t
ORIGIN      
        1 acgcggtgct ggatacgcgg acgatctcgg gattccaggg ggggtggtat ctggtgtgga
       61 acctggcggg gcacgtcaag ctgagggtca ccttgacaag cggcgccaac gcggtggtca
      121 gtggtttgtt ctttcgttag cggcgggcgt gggccagtcc ggcgcgcacg cgttcacgca
      181 gaaacgctgt caggcccttc cagtgccgca acaagttact ccgcatggcg tcagaaggca
      241 tcagaccgac gatttcgcac agccggctgc tacgcggcat tagaaccgcc gagatcgcgt
      301 tggcggtcgt gctggcgata ggtgctggac tgctcggaaa gagtttcctg caactcctcc
      361 tggcgggccc agggtatcac atcaaccgga gcaacctact ggcctggacg gttaccgggg
      421 cggcttttat gcggattccg atgatgtcga tcagtcattc cgatctcatg ccgatcagat
      481 cggagcgaag cgacgcaggg ctctctcagt gtgagatagt gatcggcatc agtcaagact
      541 tttgcttgtt ttctctatct tgatgcgacg gagtttctcg ccttcgggtg cttttgtggt
      601 tttgggcgct gacggaagga gcccgtaggg cgactggagt tagcgcccaa aaccattccg
      661 cgatccgtcc gttactcggc atctttgcct cccctaccgc ctttcttctt tcgcatcgac
      721 tctccctgta gctcgatccg gtgcgccgca tgcaccagac ggtccagaat gctgtcggct
      781 accgtcgggt cgccgatctg cgcatgccac ttcgccaccg gcaactgact ggtcaagagc
      841 gtcgacctgg tcatgtagcg ttcgtcgcag atctccagga aagcacgtcg ttcgccttcc
      901 gccattggag acatcgccca atcatcgacg atcagcacat ccacctgtcc caagcctcgc
      961 agccgcttgg catagctgcc gtccgcccgc gccagttcca gttcccgaaa caactgtgcg
     1021 gcagtggcga agtaggcggt gaatccgtcg cggcatgcct tctgaccgaa agccttcgcc
     1081 agaaacgttt tcccaatccc ggttggcccc accaggaaaa tgtgttgatg gcggcgcacc
     1141 cactccgagt cgttcatcag cgagcggacc acctgcttgt ccagacctcg cgccgcacga
     1201 aagtcgatgt cttccatgca ggctggccct tgcagtcgcc catctctgag ccgtcgctcc
     1261 aacacccggt tctgccgcca actccactgg cggtccacca gcagggcaaa acgttcctcg
     1321 aagctgagct gtgtagtctc gggttcttcc tgttgttggg taaatgcatc ggccatgccg
     1381 cgcatgcgca tggtgtacag tttctcgatc gtctgttggt tgagcatggt tctccttgtg
     1441 tgcttactga atcactggag gctcggcctc cggtggggaa tcgaagtaat cggcgccgcg
     1501 aatgttttcg tggttgaccg caggctgaac cggaggatcg ccaggcaatg gcagccgatc
     1561 cagttggttc ttgaggatcg agtccatgct ctggcagttg taggcgcgag ccctcaggca
     1621 acgccgggcg gcggcctcca tacgctcggc cggataggtt ttcgccaaac gcagaatccc
     1681 caggcatgac cgatatccct gctccggatg gcgtttggcc gccagaatcg cttccaccag
     1741 ttgccccgtg taggggccgg tttgctgcgc atcttcgatc agccgcgacg gtgtgcgccc
     1801 gacgtatttc tgatgggcct tgggcatgtg ctcggtcaac gtagtcgctt tttcccgcag
     1861 gtaggaacga acatgcgacg ccacacgcac tccgcggtgg atgatttcca ccgtctcccc
     1921 cgtcagatgc acgtccacct gctgatgcac cagtgcgtac ggcacgctgt agtaatgccg
     1981 ctccacttcg atgtgatagt cgatgttgac gcgggcgatc tcccattctc cgaactggta
     2041 ccgtgtggcc ggcagcggct tcagcgccgg ctggtccagt tgcgcgaaca gcgtggcgcg
     2101 gctgccctct cgcttgcgga atggccgctg gttgagccgg accaacagtt cggcaatggc
     2161 ctgattgact tccgcgaggg agaagaactt gcgcttgcgc aaggctgcga cgatccagcg
     2221 ttggaccacc tgaacaccca cctccgcctt ggccttctcc ctgggtcgat atggccgcgc
     2281 tggaatgatc gccacgccgt agtgctctcc caagtcgcgg taggtggggt tcagatctgg
     2341 ctcgtaatag ctgggatggg tgacggcact cttcaagtta tctgggacca cgacctcggc
     2401 cacgccaccg aaaaactccc aggcccgcat atgcgaaccg atccagttcc gcagatcctg
     2461 cccggtcgtg gcctcggcga aggtgtaact gctggcgccc agcaccgcca cgaagacagc
     2521 ggcagggtgc gcgtcgccgt tttccgggtt atgaaccgga atggtggctc cggcgtaatc
     2581 cacgaacatc ttctcgccgg cgcgatgctc ctggcgcagc accaggtcga gtttctttag
     2641 ccaccgccgg tagagatcgc agaagcggct gtagccgtag ccatcggggt tgctctcgcg
     2701 gccttcctgc cagaccaact gcagggtcag gtccttgtgc gtctgcaact cttggtggag
     2761 ttgcgtccag tccggctccg gatgcttccg ccaaatggcg ggcgcgggtc gctgcggaaa
     2821 cagcgttcgc tcgatctgct ggtcatccca gtgctccagc gccggccact tcacgccggc
     2881 cgcttgcgcg gccgacaggt actcgtggac ggtgctctgg gaaatggagc aactgcgggc
     2941 gatctggtgc tggctcagtc cgagggaatg gagccgcaga acttccttca acttgcgcat
     3001 ggtcagcctc ttctgtggca actgggagtc tccacttatc agtggaatcc agtcgccacg
     3061 gctgccctac gccgccgttc caatctcatg ccgatcacta ttccgaacta caaccgtaaa
     3121 actgatcggc ttcagttcgg aacactgatc ggcatcactt cggaatcgtg atcgacatca
     3181 gttcggaacg ctgatcgcca tccctcggaa tccgcagata gcgaggatac cggctacgaa
     3241 gtcaccgtgc aggtgaccga tagcgattcg atttacaacc agccggagga cccaaatgcc
     3301 ctagacagcg gcgcccgtcg caccagattc cgtccgctta ccttcccggc cccagccatc
     3361 ttgaatttgg acacggctcc tatccctgtc
//
LOCUS       DASRKK010000816        10084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1070632, whole genome shotgun sequence.
ACCESSION   DASRKK010000816 DASRKK010000000
VERSION     DASRKK010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070632"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..849)
                     /locus_tag="VFC15_19525"
     CDS             complement(40..849)
                     /locus_tag="VFC15_19525"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZM12404.1"
                     /translation="MPQSQAITTTSTKYQNILFDIEGQIAIVTLNRPQRRNALSLELM
                     TELIDCLNEIGRDRSLRVVMLRAAGKVFSSGHDLSEMVGRNINEYRQVFDVCTELMTK
                     VQSIPQPVIAEVQGIATAAGCQLVATCDLALASDQAAFATPGVKIGLFCTTPMVALSR
                     AVGRKRALQMLLTGEMISAATAAEWGLINMVVPAEELAEQCHNLATRIAEASSLTVAL
                     GKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEGISAFLEKRRACWTGK"
     gene            complement(849..2396)
                     /locus_tag="VFC15_19530"
     CDS             complement(849..2396)
                     /locus_tag="VFC15_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM12405.1"
                     /translation="MISALTPLEFLSRNAIAYCDSTAEVDGDRRFTYSEMQQRVHSLG
                     AALQQAGIAPGDRVAVLARNGLLPLEAHFAVPLIGAVLVTLNIRLQGGELATILNHCG
                     ARILLGDTDLLAPLQAVRKDIPKLERMISDYEAFLGEGSNPVVPHAPREEDVISINYT
                     SGTTGVPKGVMYTHRGAYLNAIGEVVEHGLSYRSVYLWTLPMFHCNGWCFTWAITAMG
                     ARHICLPEMNAERAVKLIQGEGVTHLCGAPIVVRTLTEFCSSHGIRFDAGLKMVIAAA
                     PPPPAVIRAAEAIGIELTHVYGLTETYGPHSVCAWHTDWDDVPLEERALLKARQGVPY
                     TVFGTDMRVVDEDMRDVPADGLRVGEVVMRGNNVMLGYYLEPEATATAFRGGWFHSGD
                     LAVMHPDGYIEVRDRAKDIIISGGENISSLEVERVLYEHPAVLEAAIVAWPDEKWGEV
                     PHAYVLVREDVVVSEQDLIEFCRERLAHFKCPKAIHFGALPKTSTGKIRKNVLRHQSR
                     AAVEGDS"
     gene            2769..4118
                     /locus_tag="VFC15_19535"
     CDS             2769..4118
                     /locus_tag="VFC15_19535"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM12406.1"
                     /translation="MSAEHFDTIQEREKGLQRGLTSAQLTMIAIGGAIGTGLFLGSSF
                     AIGFAGPAVIVSYAIGAFIALLLMGCLAEMTVAHPTSGSFGAWAEFYIGPLAGFLVRY
                     AYWSAIVFAVGTEVTAVAIYMRYWFAGVPGWLWIVGFSSILISVNVLNVKVFGAVEYS
                     LSALKVTAILVFILLGAWIVTGTGLSNYTAQGGFFPKGLWGMWVAVIVSIFSYLSIEM
                     IAVAAGEAANPEQAIISAFRSTVVRLVVFYLLTLAIILAIAPWTESGKGESPFVTVMA
                     RSHVPFAAGVVNFVILVAALSAMNSQLYITTRMMFSLARAGHAPRRFGELSTRGTPVS
                     ALLLSSIGIALATVVSILFPNAAFTLMMAISMFGAMFTWMMIFITHLYFRRAHAGAKS
                     AFRMWGYPYSSLLGAGLMAAILLSTAFTAEFRMTLIYGLPVLALFWAAWRLRRQSVS"
     gene            4115..5278
                     /locus_tag="VFC15_19540"
     CDS             4115..5278
                     /locus_tag="VFC15_19540"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM12407.1"
                     /translation="MNSMDDLLLKWRKEFPILAHATYMISHSLGAMPKQAAESLQQFA
                     DTWATRGIRAWEEGWWDMPVTVGDLIASIIGAGQGEVVMHQNVSICQWIVASCFDWRG
                     PRNKLVTDGLNFPSNDYIYYGLERQGARVVRVQSDDGLTLPTETMLAAIDEETRLVSV
                     SHVAFRSSYVQDLAAITRRAHEVGAMVVADLYQSAGCLPVNVRELQVDFATGGSVKWL
                     CGGPGAGYLYVRRDSWDQLDPAATGWQAHREPFAFEPGPIKFADNAMRFLNGTPNVPA
                     MYSAKTGYQIIQQVGVENIRAKSVRQTQLLMDLADESEIPVRTCREAARRGGVVILDV
                     PNGKDVTQELIRREILVDYRPQAGIRIAPHFYNTDEEVEHTVRQIREIAYAQA"
     gene            complement(5592..6032)
                     /locus_tag="VFC15_19545"
     CDS             complement(5592..6032)
                     /locus_tag="VFC15_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12408.1"
                     /translation="MPAAANGDISAYVTDATDLVVDINGYFAPEVPGAKALYTTAPCR
                     VLDIRPNAFNGQIPINVASGPCGIPSAAQAYVLNATVVPQTKLGFLTLWPDGVSRPLV
                     STLNAVDGKITSNMAIVPTSNGSIDAFGTNPTNLVLDLSGYFGP"
     gene            complement(6275..6781)
                     /locus_tag="VFC15_19550"
     CDS             complement(6275..6781)
                     /locus_tag="VFC15_19550"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM12409.1"
                     /translation="MKRQAVAVLLTILVLGFVSAFAQSGAPGVAKQVRDNFNDVDRKI
                     LDMARDWPADNYDYKLRKEMRSFGAVLVHLASGNVYAAKIGRGEKAKWDELDPAKYPD
                     KAAVVALLEKSFADSEAVLKGLPDASFGKSVEPWLSVMEHSGEHYGLLVAYYRANGMT
                     PPESRPKK"
     gene            complement(7081..8226)
                     /locus_tag="VFC15_19555"
     CDS             complement(7081..8226)
                     /locus_tag="VFC15_19555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HZM12410.1"
                     /translation="MSLKKWALRLWMLPLAVFMVAATPTVLYSAPNTYSQQNLVSDLP
                     GVAKFLDPDLVNPWGMAFSATSPIWVSDNGSGLATIYNGAGVKQGLVVSIPAPGGGQG
                     APTGQVFNGGSGFNSDRFLFSTEDGTIAGWRGALGTTAELGADLSGSGAVFKGLALAT
                     SGSNSYLYATDFHNGTVDVFDSTFTKVTLTGNFSDPTIPTGFAPFGIEASGGKLFVTY
                     AMQDAAKHDDVPCPGCGFIDVFNADGTFVQRLVSMGALNSPWGLALAPGHFGTFSNDL
                     LVGNFGDGTINAFDPITGAQIGTIDGRNGMPLVNLGLWGLAFGNGAQGTSTNTLYFTA
                     GIPGPGQIEDHGLFGAITTPEPGTLTLLGSGLASLFGYGWRRGKRTA"
     gene            8508..9188
                     /locus_tag="VFC15_19560"
     CDS             8508..9188
                     /locus_tag="VFC15_19560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM12411.1"
                     /translation="MDKVLAIEDDPAVQRALRRTFETAGFDITIAEDGATAMDVFRAT
                     TPRVVILDLRLPGRSGQDICREIKRLAASVPILVLSAASDVVDKVLLLELGADDYVTK
                     PFSPRELLARVRAAIRRLNQSPDKGDIFNFDDVEVNFSKMELWKAGKLVPLTPQEFKA
                     LRFFLNNEERVVSRAELLNEVWGYNSYPTTRTVDTHILRLRQKLEKDPGEPIHFRTVH
                     GTGYKFVR"
     gene            9249..>10084
                     /locus_tag="VFC15_19565"
     CDS             9249..>10084
                     /locus_tag="VFC15_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZM12412.1"
                     /translation="MLLTSAGLTAVSLLIVQRSVENQVRRDLRVDLANSVETFRNFQR
                     ERENSLARSAELMADLPVLKALMTSRHAPTIQDASTELFRLSGGDLFALVDQSNQIVG
                     FHARTPGITLEQAQELRTRKTGPDDSLQWWLAGGHLYEVFLVPIYFGPKASNSILGTV
                     AIGYEINEIVAHEVARVAGGQVAVVCSGKDVVSTINTADVDELQQALAAHDAPAGPLD
                     VKLGNESFVVASLALNEGAPPVTMFVMKSYDQAAQFLSHLRQLLLGVGLGAVLIGSLL
                     VY"
BASE COUNT         1994 a         2937 c         3031 g         2122 t
ORIGIN      
        1 ttactaatga gtctcaagag tgcagcacga agcagtactc tactttcccg tccaacacgc
       61 gcgacgtttt tccagaaatg ccgagatgcc ctcctgcgcg tcggcggcga gcgcattcat
      121 gctcatcacc tctttggcgt aggcataggc tttgggctgg tcgagatcaa tctgcgtgta
      181 gaacgcctgc ttgcccagtg cgactgtcaa tgagctagct tcggcaatac gagtggcgag
      241 attgtggcat tgctccgcca gttcctcggc aggcacgacc atattgatca gtccccattc
      301 cgccgccgtc gccgcagaga tcatctcgcc ggtcagcaac atttgtagcg cgcgtttgcg
      361 cccgacagct cgactgaggg ccaccatcgg tgtggtgcag aaaagtccaa tcttgacgcc
      421 gggcgtggca aatgccgcct ggtcggaggc caaggccaaa tcgcaggtcg ccaccaattg
      481 gcatccggcc gcggtcgcga tgccctgcac ctccgcgatc acgggctgag ggatcgattg
      541 cactttcgtc atcaactccg tacagacatc gaacacctgg cggtactcgt tgatgttgcg
      601 tcccaccatc tcgctcaaat cgtggcctga cgaaaatacc tttcccgccg cccgtaacat
      661 caccacacgc agggagcggt cgcgcccgat ttcgttcaag cagtcgatca gctcggtcat
      721 caactccagc gatagagcat tgcgccgttg cggccggttc agcgtcacga tcgcaatctg
      781 tccctcgatg tcgaacagga tgttctggta cttagtagac gtcgtggtga tggcttgcga
      841 ctgtggcatc acgaatctcc ttctactgca gcgcgcgatt ggtggcgcag cacgttcttg
      901 cggatcttgc ccgtggacgt tttgggcaat gcaccaaagt gtatcgcctt cgggcattta
      961 aagtgcgcca accgctcgcg gcagaactcg atcaggtcct gctcgctgac cacgacatct
     1021 tccctgacca gcacgtaggc atgcggcacc tcgccccact tctcgtcggg ccaggcaaca
     1081 atcgccgcct ccagcacagc gggatgctcg tacaacaccc gctccacctc aagcgaggaa
     1141 atattctccc caccggagat gatgatgtcc ttggcgcggt cgcgcacctc gatataaccg
     1201 tcggggtgca ttaccgccag atctccggag tggaaccagc cgccacggaa tgcggtcgcg
     1261 gttgcctcgg gctcgaggta gtagcccagc atcacgttgt tgccgcgcat cactacttct
     1321 cccaccctga gtccgtcagc ggggacgtcg cgcatgtctt cgtccacgac ccgcatgtca
     1381 gtcccaaaaa ccgtgtacgg tacgccctgc cgcgctttga gcagcgcacg ctcttccagc
     1441 ggcacatcgt cccagtccgt gtgccaggca cataccgagt gcggcccgta ggtttcagtg
     1501 agcccataga cgtgcgtcaa ttcgatgcca atcgcttcgg ctgcccggat cacggccggt
     1561 ggtggaggcg ctgccgcgat caccatcttg agtccagcgt cgaaacgaat cccgtgactt
     1621 gagcagaact cagtgagcgt gcgcaccacg atgggagcac cgcagaggtg agtgacgccc
     1681 tccccctgga tcagcttaac ggcgcgctcg gcattcatct cgggaaggca tatgtgccgc
     1741 gctcccatgg ccgtaatcgc ccaggtgaag caccagccgt tgcagtggaa catgggtagc
     1801 gtccacaggt aaaccgaccg gtacgacagt ccgtgttcaa ccacctcgcc aatggcattg
     1861 aggtacgcgc cgcgatgggt gtacatcacg cctttgggca ctccagttgt gccgctggtg
     1921 tagttgatcg aaatgacgtc ctcctcgcgc ggcgcgtgcg gcacgacggg attgctaccc
     1981 tcgccgagga aagcctcgta atcggagatc attcgttcca atttcggaat gtcctttcga
     2041 acggcctgca gcggtgcgag caggtcagta tcgccgagca aaattctggc gccgcaatgg
     2101 ttcaggatgg tagccaactc gccgccttgc aaccgaatgt tgagcgtgac cagcaccgca
     2161 ccgatgagcg ggactgcgaa gtgagcttcc aagggcagca gtccattgcg cgccaacacc
     2221 gcgacccgat ccccgggcgc aataccagct tgctgcaaag ccgcgcccaa agaatgaacg
     2281 cgctgctgca tctcggagta ggtgaagcgc cggtcgccgt ccacttcagc cgtgctgtcg
     2341 cagtacgcga tcgcattgcg ggaaagaaat tccaacggag taagggccga tatcataaga
     2401 accgcctggc ttgttgaatt tggcgcgaat tatatagcag aggctgtggg gcgtctgtga
     2461 taccgaattc aacgcctgag aaaatgcggc cagagcgcct cagatgaacg cgtggcatca
     2521 ggaccttgtt ccagatgtcg tcgccgagaa tggcgagcct ccggtgatgc atcggcgtat
     2581 cgatgaatcc cggacacacg atgttgaccc ggattccatg cggcgccagc tccgcggacg
     2641 gcgatgttct tccgatgcgc tgttaggatt gcgattatag cgaaggggaa agccagtcgc
     2701 ctgtgacgaa ggcgacccga tcgggttcga gttgctcgct ggtacaccgt gatggctctg
     2761 caaaactgat gagcgctgaa cacttcgata caattcagga gcgcgaaaag ggactgcaac
     2821 gaggattgac ctcggcgcag ctcacgatga tcgccatcgg cggcgcaatc ggcactggcc
     2881 tgtttcttgg cagcagtttt gcaatcggct ttgccgggcc ggctgtcatt gtcagctacg
     2941 ctattggcgc cttcatcgct ctcctgctca tggggtgcct ggccgaaatg acggtggcgc
     3001 atccaacatc gggctcattt ggcgcgtggg cggagttcta catcgggccg ctggccggct
     3061 tcctggttcg ctatgcctac tggtcggcaa ttgtctttgc ggtcggtacg gaagtgaccg
     3121 cggtcgccat ctacatgcgc tattggttcg cgggggtgcc cggttggctt tggatcgtag
     3181 gtttctcctc catcttgatc tccgtgaacg tgctcaacgt gaaagtattt ggcgcagtgg
     3241 agtactcact gtcggcgttg aaagtgacgg ccatcctcgt gttcatcctt ctgggcgcat
     3301 ggatcgtgac tggcaccggc ttgtccaact acaccgccca aggtgggttc ttccccaaag
     3361 gcctgtgggg tatgtgggtg gcggtgattg tctcgatttt cagctacttg agcattgaga
     3421 tgattgcggt cgcagcgggg gaagccgcga atcccgagca ggctatcatc agcgcctttc
     3481 gctctaccgt tgtgcgcctc gttgtcttct atcttctgac attggctatc attctcgcga
     3541 tcgctccgtg gacggaatcc ggaaagggcg aaagcccgtt tgtgaccgtg atggcgcgtt
     3601 cgcacgttcc gtttgcggcc ggcgtggtga atttcgtgat tctggtagcg gcgctttcgg
     3661 ccatgaacag ccagttgtac atcaccacgc ggatgatgtt cagcctggcg cgggccggtc
     3721 acgcgccgcg gcgattcggg gaactgagca cacgcggaac gccggtttcg gcgctgctgt
     3781 tgtcgtcgat tggcatcgcg ctcgcgaccg tggtaagtat cctgtttccg aacgcagcct
     3841 tcacgctgat gatggccatc tcgatgtttg gcgcgatgtt cacctggatg atgattttca
     3901 taactcacct gtatttccgt cgcgcgcacg ccggcgcgaa atccgcgttt cgcatgtggg
     3961 gttatcccta ttccagcctg ctgggcgctg gattaatggc ggccatcctg ctgagcacgg
     4021 cgtttacggc ggaattccgc atgaccctga tttacggctt gccggtgctg gccctgttct
     4081 gggcagcgtg gcggctcagg agacagtcgg tatcatgaat tccatggatg atctattgct
     4141 caagtggcgg aaggagttcc ccatcctcgc ccacgccacg tacatgatta gccattcgct
     4201 gggcgcgatg ccgaaacagg cggctgagtc gctgcaacag tttgccgaca cttgggctac
     4261 gcgcggtatt cgagcctggg aggaaggctg gtgggacatg ccggtcaccg tcggcgacct
     4321 gattgcctcc atcattggcg ccggccaggg cgaagtcgtc atgcatcaga acgtctcgat
     4381 ctgtcagtgg attgtggcgt cgtgcttcga ttggcgcggt ccgcgcaaca agctcgttac
     4441 cgacggcctg aacttcccat cgaacgatta catctattac ggtctcgagc ggcagggggc
     4501 gcgcgtggtg cgcgtgcaat cggacgacgg cttgacgttg ccaacagaaa ccatgctggc
     4561 agcgatcgat gaagaaacgc gactggtgtc ggtgtcgcac gtggcgttcc gcagttcgta
     4621 cgtgcaggat ctagcggcaa ttaccagacg ggcgcacgag gtgggggcca tggtggtggc
     4681 cgatctgtat caatcggccg gatgcctgcc ggtgaatgtg cgggaattgc aggttgactt
     4741 cgcgactggc ggctctgtga agtggttgtg cggcggcccg ggcgcgggct acctgtatgt
     4801 gcgaagggat tcgtgggacc aactggatcc cgcggccacg ggatggcagg cgcaccgcga
     4861 gccgtttgcg tttgagcctg ggccgatcaa gttcgccgac aatgccatgc gcttcctgaa
     4921 tggtacgccg aatgttcccg cgatgtactc ggcgaaaacg ggataccaga tcattcaaca
     4981 ggtcggagtg gagaacatcc gtgcgaagtc tgtgcggcag actcagttgc tcatggacct
     5041 ggcggatgaa tcggagatcc ccgtacgcac ctgccgcgaa gccgcacgcc gtggcggagt
     5101 tgtcattctg gatgtgccga acggcaagga cgtaacgcag gaactgattc gacgcgagat
     5161 ccttgtggac tacaggccgc aggccggcat tcgcatcgca ccgcacttct acaacacgga
     5221 tgaagaagtg gagcacaccg tgaggcagat tcgcgagatc gcctatgccc aagcgtagtc
     5281 gatctgcggt ggtgccggcc acgacgcatc ccgctggtct cgacaacatc ccaacctctt
     5341 tgttgaggtt tagtacactc ccgcggcaat ccgttcacat cgagaacgcg tttgctgtct
     5401 cggttgcagc ggacaaagag cggacaaggt ttttcgaatt tcggatgtga gcgttgaggc
     5461 ggaaagactg aggtgtggta agaagggtcg gcaaaacctt tattcgtggg tccgattccc
     5521 acccgcgcct gcaacgtttt caaagattcc ccggcccacg gcctacttcc gtcccctaat
     5581 tgcaggcgaa gctatggccc aaagtatccg gagagatcca ggaccaagtt ggtagggttc
     5641 gtcccgaacg cgtcgatcga accattgctt gtgggaacta tcgccatgtt cgaggtgatc
     5701 ttcccgtcga ctgcgttcag ggttgaaacc aagggccgac tcacgccatc gggccacagc
     5761 gtcagaaagc cgagtttggt ttggggaacg acggtggcgt ttagaacata tgcctgtgcc
     5821 gcgctgggaa tgccacatgg gccgctagca acgttgatgg gaatctgtcc attgaatgcg
     5881 tttggccgta tgtccaaaac tcggcacggg gcggtagtat acagcgcctt agctcctggc
     5941 acttcgggcg cgaagtatcc gttaatgtcg acgaccaggt cggtcgcgtc ggtgacatag
     6001 gcgctgatgt cgccgttagc ggcagcaggc acgatggccg cgtttgccgt gatcaggtgg
     6061 acgctgaagg aaagggctat cggatgatcg caggaaaggc aaaggcgatt cgcgcaggaa
     6121 acgcttcggt tcggtgaaga ctctgctggg cggcccagtt aagcgcttct gctgcgggcg
     6181 ccccatccta accgtcgtag gttggcaagg cagttatccg acaccgctgg ctttcgcccc
     6241 cgcgattgtc ttccaaccgc tcgggtaagc tgctctactt tttcggccgc gactctggag
     6301 gcgtcatccc gttcgcgcga tagtaggcga ccagcaaccc atagtgttcg ccggaatgtt
     6361 ccatcacgct cagccagggc tcgaccgact tgccgaacga tgcgtcaggc aggcccttca
     6421 acacggcttc ggaatcggcg aacgacttct ccagcagcgc caccaccgcg gccttgtccg
     6481 ggtacttggc gggatcgagt tcgtcccact tcgctttctc gccgcggccg attttggcgg
     6541 catagacgtt gcccgacgca aggtgcacca gcacggcgcc aaacgaacgc atctccttcc
     6601 gcagtttgta gtcataattg tcagccggcc agtcgcgggc catgtccagg atcttgcggt
     6661 caacatcgtt aaagttgtcg cgcacttgtt tcgcgactcc cggggcgcca ctctgagcga
     6721 atgcggacac gaaccccaat accaggatgg tgagcaagac tgcaaccgct tgacgtttca
     6781 tggaccctcc tcctagacaa ccgacgcagc atatcagaaa ccgtcaccac ggaaatacat
     6841 tcaggtcgga tgtgcgcggc gtcgtggcgc gattttgaac ggggcggggt gttttgtttc
     6901 aacaacacgg caaaccagtt acagctcact ctggattatc aacgcagcaa gtaactcgcg
     6961 ccttacgccc aaaaaaatcg agccggagtg cgcggctccg gctcagtgcg ttttgtcagg
     7021 agagttccag gtgcgggaat cctcagtgta ttgtccgcga ggatttgctt tcaggatcga
     7081 tcaggctgtg cgtttccccc tgcgccaccc gtaaccgaac aagctcgcta gtccgctgcc
     7141 gagcagggtg agagtgccag gctcgggagt tgttatggca ccgaacagac catggtcctc
     7201 gatctgacca ggcccgggaa tccccgccgt gaaatacaga gtgttggtac ttgtgccttg
     7261 ggcgccattc ccaaaggcaa ggccccagag accgagattg acgagaggca tgccgttgcg
     7321 gccatctatt gttccgatct gggctccggt tatagggtcg aacgcattga tggtgccgtc
     7381 gccgaagttt ccaactaaca agtcattgct aaacgtacca aaatgtcccg gtgccaatgc
     7441 caggccccag ggcgagttca gagcgcccat cgaaaccagt cgctggacga aggtgccatc
     7501 agcgttgaac acgtcgatga acccgcatcc cggacaaggg acgtcatcgt gcttggcggc
     7561 gtcctgcatc gcataagtca cgaacaactt gccgccggaa gcttcaattc caaacggagc
     7621 gaagccggtg ggaatggtgg gatcggagaa gttaccagtg agagttacct tggtgaaggt
     7681 tgagtcaaaa acatcaaccg tcccgttgtg gaagtcggta gcatagaggt agctgttgga
     7741 gccgctggtg gctagcgcca accccttgaa gacggcgcct gatcccgaca ggtcagcccc
     7801 caattcggca gttgtcccca gggcgcctct ccatcccgct atcgttccgt cctccgtaga
     7861 aaagagaaag cggtcgctat tgaagcctga gccgccattg aagacctggc cggttggtgc
     7921 accttgtcct ccgcctggcg caggaatcga tacgaccagt ccctgtttca cgccggcgcc
     7981 gttatagatc gttgccagtc cacttccatt gtcggatacc caaatgggac tggttgccga
     8041 aaaagccatc ccccacggat tcaccaggtc tgggtccaag aacttggcaa cgccgggtaa
     8101 gtcagaaacc aggttctgct ggctgtaggt gttgggtgcc gagtagagca ccgttggggt
     8161 tgcagctacc atgaacacgg ccaggggcag catccaaagg cgtagggccc acttcttgag
     8221 actcattgca ttacctcctc gagattgatc ggtatcgatc gaccacgcaa atctatcgtc
     8281 cggcaaaaac ggcgttgtca tcgggttgta gaaaggttgt caacaaaatg tgtcggcgaa
     8341 tgcggcagcg aaatcatcaa ctcatagtcc gagtgcctat gcgctcccgt tgttgcattc
     8401 aatggcgtgc gatgtcacgg aattgtgaac acatcgttga ctgccggcat cgattggtat
     8461 agctgttgaa ttacacaggc cgctcataac ctggggcaga ggagagaatg gacaaggttc
     8521 ttgcgatcga agacgatccc gcggtgcagc gcgcactgcg ccggacgttt gaaaccgccg
     8581 ggtttgacat aactattgcg gaagacggcg cgaccgccat ggacgtgttc cgcgccacca
     8641 ctccgcgtgt ggtgattctc gacctgcgcc tgcccggccg ctcgggccag gacatttgcc
     8701 gcgagatcaa gcgtctggcg gcgagcgtac cgatcctcgt cctgagcgcg gccagcgacg
     8761 tggtcgacaa ggtgctgctg ctggagctgg gtgccgacga ctatgtgacc aaaccgttca
     8821 gcccgcgcga actgctggcg cgagtccgcg ctgccattcg acggctgaac cagagtcccg
     8881 acaaaggcga catcttcaac ttcgacgacg tcgaagtgaa tttttccaag atggagctgt
     8941 ggaaggcggg caagctggtt cccctgaccc cacaggagtt caaggcgctg cgcttcttcc
     9001 tgaacaacga ggagcgggtg gtttcgcgcg cggagctgtt gaacgaggtt tggggataca
     9061 acagctatcc caccacgcgc accgtggaca cgcatatcct gcgcttgcgc cagaagctcg
     9121 agaaggaccc cggcgagccc atacacttcc gcaccgtcca tggcacagga tataaatttg
     9181 tgcgataaag caagcagcac gagtcgagga gatcctccat acggcttaga accaaattcc
     9241 tgattgccat gctgctgacc tccgccggat tgacggcggt gtcgctgttg atcgtgcagc
     9301 gcagcgtcga gaaccaggta cgccgcgacc tgcgagtcga tctggccaac tcggtcgaga
     9361 cctttcgcaa tttccagcgc gagcgtgaga attccctggc ccgctcggcc gagctgatgg
     9421 ccgacctgcc cgtgctgaag gcgctgatga cgtcgcgcca tgcccccacc atccaggacg
     9481 cctcgaccga gttgttccgg ctttcgggtg gcgatctttt tgcgctggtt gaccagtcga
     9541 accagattgt gggatttcat gcccgcactc ctggaatcac cctggagcag gcccaggaat
     9601 tgcgcacccg taagactggc cccgacgatt cgctgcagtg gtggcttgcc ggcggccatc
     9661 tctacgaagt gtttctggtg ccgatttatt tcgggcccaa ggccagcaac tcaattctgg
     9721 gcaccgtcgc catcggctat gaaatcaatg aaattgtcgc ccacgaagta gcgcgcgtgg
     9781 ccggcggcca ggtggcagtg gtgtgctccg gcaaagacgt tgtgagtacc atcaacacgg
     9841 ccgatgttga cgaactgcaa caagcgctgg cggcgcacga cgccccggcc ggcccgttgg
     9901 atgtgaaact ggggaacgag agctttgtgg tggcctcgct tgcgctcaac gaaggcgcgc
     9961 ctccggtcac catgtttgtc atgaaatcgt acgaccaggc ggcgcagttt ctcagccatc
    10021 tgcggcaact tttgctgggc gtcggtctgg gcgcggtcct gattgggagc ctgctggtat
    10081 attt
//
LOCUS       DASRKK010000817         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1265910, whole genome shotgun sequence.
ACCESSION   DASRKK010000817 DASRKK010000000
VERSION     DASRKK010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265910"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFC15_19570"
     CDS             complement(<1..818)
                     /locus_tag="VFC15_19570"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZM12413.1"
                     /translation="MSKSIGQAEVLVAGGGPAGLAAAIAARQAGFQVTVVDCAQPPID
                     KACGEGIMPDGLAALRQLGVRIRPGQAAPFRGIRFLNDDDRVEADFPHGTGYGIRRTV
                     LHQLLIDRATELGASLYWGARITGLSAQGVAIGGQHVGCRWLVCADGYNSLLRRSAGL
                     DVSRSAQRRFGFRRHYRLAPWSELVEVHWGNCGQMYVTPVSDNEICVAFITRSLGLGW
                     EEALPHFPGLWAHLKSVAPGNRHLGALSISRRLATVQLGKVALVGEASGSVDAVT"
     gene            complement(824..1906)
                     /locus_tag="VFC15_19575"
     CDS             complement(824..1906)
                     /locus_tag="VFC15_19575"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZM12414.1"
                     /translation="MEPREEGNAQMTRIAGVASAFPRQYYRQEQMAEALKRHWGTKLD
                     NPKVLDRLLTRVGVDGRYLALPIEAYDDLTTWGKSNNAWIEVAEDLAEQSICRALTRA
                     GLCEADLDAIFFVSVTGVASPSIDARLMNRMNLRPDIKRNPIFGLGCVAGAAGLARAA
                     DYVRAFPKQCAVLVSVELCSLTWQRKDLSVANLISSGLFGDGSAAVIVAGSERSASGP
                     RVLGSRSSFYRGTEDVMGWDISEEGFRIVLSTKVPDMVRKHLVRDVDGFLSDYDLNRS
                     DIDTWIMHTGGPKVLDATAESLDLPEGALDVSWQTLRRVGNLSSASVLLVLEEFMNRR
                     RPATGSYSILAAMGPGFCSELVLLRW"
BASE COUNT          550 a          789 c          681 g          445 t
ORIGIN      
        1 gtaaccgcgt caaccgaacc cgatgcctct cccaccaggg cgaccttgcc gagttgaacg
       61 gtcgcgaggc gacggctgat tgacaaagct cccaagtggc gattgccggg cgctacactc
      121 ttcaggtgag cccacaatcc ggggaagtgg gggagcgcct cttcccatcc aagcccaagg
      181 ctgcgcgtaa taaacgccac acagatctcg ttgtccgaca ccggcgtgac gtacatctga
      241 ccgcaattgc cccagtgtac ttctaccaac tctgaccagg gcgccaatct gtagtgcctg
      301 cggaatccaa atcgccgttg tgcggagcgg gatacgtcga gacctgcact tctgcggagc
      361 agagagttgt atccgtcagc gcagaccaac caacgacagc ccacgtgctg cccgccgatg
      421 gcaactccct gggcggacag gcccgtgatc cgtgcacccc aatacaagga agcacccagc
      481 tcagtcgccc ggtcgatcag cagttgatgc aagacggtgc gccgaattcc atagccggta
      541 ccgtgcggga agtccgcctc cacccgatcg tcgtcgttaa ggaaacgaat gccgcgaaat
      601 ggcgctgctt gcccgggacg tatacgaacg cccagttggc gaagtgctgc caggccgtca
      661 ggcatgatgc cctcaccgca cgctttgtcg atgggaggct gcgcacaatc gacgacggtg
      721 acctgaaaac ccgcttgtcg agctgcgata gcagccgcca gtccagcggg gcctccgccg
      781 gcaaccagca cttcagcttg accgatggat ttggacattg cctctaccat cgcagcagca
      841 caagttccga gcagaaacct gggcccatag cggcaagaat gctgtaactg ccggtcgccg
      901 ggcggcgccg gttcatgaac tcttccagca ccagcagtac cgaggcagac gagagattgc
      961 ccacgcgccg cagagtctgc cacgaaacat cgagcgcacc ctcgggaagg tccaatgact
     1021 cagccgtagc gtccagaacc ttgggaccgc cagtatgcat gatccacgtg tcgatatcgc
     1081 tgcgattgag gtcgtagtcg gagaggaagc cgtcaacatc tcgcaccaga tgcttgcgca
     1141 ccatgtccgg aaccttggtc gacagcacga tcctaaaacc ttcttccgag atgtcccatc
     1201 ccatcacatc ttctgtgccg cgataaaacg atgatctgct gccgagcacc cggggaccgg
     1261 aggcactgcg ctcgctcccg gcgacgatca ccgccgcaga gccgtcgccg aagagcccgg
     1321 aggaaatgag attggcgacc gagaggtcct tcctctgcca cgtgagcgag cagagttcca
     1381 ccgaaaccaa caccgcgcac tgcttgggga atgcgcggac atagtctgca gcgcgagcca
     1441 gtcccgccgc gcccgcgaca caccctaaac cgaagattgg atttcgcttg atgtccgggc
     1501 gcagattcat gcggttcatc aagcgcgcgt caatcgaggg actcgccaca cctgtaaccg
     1561 agacgaagaa aatcgcatcc aggtcagctt cgcaaagccc ggcgcgcgtg agcgcgcggc
     1621 agatcgactg ttccgcaaga tcttcggcga cctcgatcca cgcgttgttc gactttcccc
     1681 acgtggtgag gtcgtcatag gcttcgatgg gtaaagccaa atatcgtcca tcgacgccca
     1741 ctcgcgtcag tagacggtcg agaacctttg gattgtccaa ctttgtgccc cagtgccgct
     1801 taagggcctc cgccatttgt tcctggcgat aatactggcg tggaaatgca ctggctacgc
     1861 ctgcgattcg cgtcatctgc gcgttgcctt cttcccttgg ttcaatccgt ttgccggagg
     1921 aaaatacccc gttgctgcca gactagcggc tagacatgaa gcgtctattt cctgcgcatg
     1981 acaagttgtt catctgctag ccaggggtgg caatgctttg acgtatccgt cgcgcggtcg
     2041 tgttcggaac gtatcgtcgg ctaaccggaa gttatcccga aaccggcttc ggggagatta
     2101 ctccccataa gttggccagc atccggtgac ggcacgtcac gccaacttgc cgcctaacgc
     2161 ccaacccgtg ctgttgaaga actccacagg tcgcggccac aaggacggaa cgccgactac
     2221 acgtccaagt gacttccccg aagcagacct ctggcccgcg tcgatacgct gtcgtgagca
     2281 cgccaacttg cgacctaaac cgaacgggcc gctcatccgg gattaggtgc gagatggcga
     2341 tttctcaaca gtaaacggcc aaaaaccgag actcacacac gcctcgccga aaaccaatgg
     2401 cccttcacta ctgccgagga aagccactct accggacaat acaaactgag acacgacaga
     2461 aagca
//
LOCUS       DASRKK010000818         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1266015, whole genome shotgun sequence.
ACCESSION   DASRKK010000818 DASRKK010000000
VERSION     DASRKK010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266015"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..934
                     /locus_tag="VFC15_19580"
     CDS             443..934
                     /locus_tag="VFC15_19580"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM12415.1"
                     /translation="MTGNELANMYEFSFGAIKRNLDDVTNEESLVQPPGAGNCLNWVL
                     GHIVVARNMVLSLAGGPPVVGDEVAAHYRRGSEPLRPGDKVPDLGTLRGLLSDTQHQL
                     VPALAALSDEALALPVPEQLRRPPLTGSVADALIRLHYHEGYHNGQIGLLRRLVGKEG
                     AIK"
     gene            complement(1190..>2984)
                     /locus_tag="VFC15_19585"
     CDS             complement(1190..>2984)
                     /locus_tag="VFC15_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZM12416.1"
                     /translation="AFCYTALNFGESRAHVLLELRVENYAVIDNVAVEFAPGLNLLTG
                     ETGAGKSILIDALALLLGDKASTDTIRHGTDKAVVSAVFEAESKALDAVLEANGLDSN
                     AEDGQLILRREIATGGKGRVFVNNQPATVTVLKQLAPHLAVIHAQNVAVLAFDAASRL
                     ALLDAFAGVDTTTVAAAFEDWNQVRHRIADLERDEHDRLRLLDLWSFQRKEITDARVQ
                     PDEDTKLEAEKRVLANSEKVFTAAMAAYDALYESNASATASIRVATKHIEELSRFDEK
                     FRPSLPELESARITLEDIGQELRDYAEGIEASPARLAEVEDRLALLDRLKRKYGPTLD
                     DVIALGEEVDRKLNEMENKDEVLHALKIELANAGAKYLEAARSLSRHRYEAARKLEKM
                     VESEINELAMKARFKVEVSGSDEEGNWSARGFDQVQYSISANPGEPLGPVEEIASGGE
                     LSRVMLALKTSVEEGSAGRGRHNGARGDRGAPPFSPASGERVGSQRTLVFDEIDSGIG
                     GRAAEAVGKKLKQLARTKQVLCITHLPQIASFADQHYLIEKRESGGRMKTSVRPLNPD
                     ERTREIARMMSGAKLTDTSLKHAEQMLKANR"
BASE COUNT          566 a          949 c          852 g          617 t
ORIGIN      
        1 gcggcttcaa taaaagtaag gttttggcgt tactttgagt cattctttat gctgtccagt
       61 atacgagtag aaacccggtt gtccccacag ttactctatt gtagcagcgc tggaatcacc
      121 aaggtaacga cctgaaatgg cacaatttag cagcctaccg cgcaaatttc gcagccaacg
      181 ggaaccgcct gggatctagc cgcgaagcgg cgacatttgt tttagccgcg ggcgtaagcc
      241 cgcggtcaag tggggaaaac gaccgagccg cggagcggcg gcacctgcta cgacacggac
      301 tcctaaggga cggcacagtt ctcacccacc cccttcaggc ccgaggaatc tgcggtttct
      361 tttcagcgcg acgagttttt cagcagcccg catagtcagt gacaggccac actgtacaat
      421 ccttgggcag gagagctcaa tcatgaccgg caacgaactc gcaaacatgt acgaattcag
      481 cttcggagcc atcaaacgca accttgatga cgtcaccaac gaagagagcc tcgtgcagcc
      541 gccgggcgcc ggcaattgcc tgaactgggt cctgggacac atcgtcgttg cccgcaacat
      601 ggtcctgagc cttgccggcg gccctcccgt ggtgggggac gaggtggccg cgcactatcg
      661 tcgcggatcg gagccgctcc ggcccggcga caaggtgcct gacctgggta cgctccgtgg
      721 ccttctaagc gatacgcagc atcaactggt tccagcgctc gcggcgctct cggacgaggc
      781 attggctctt cccgtcccgg agcagctccg gcgaccgccg ctcacaggtt ccgtcgcgga
      841 tgccctgatt cgtctccatt accacgaggg ttatcacaac ggacagattg gactgttgcg
      901 caggctggtg ggaaaagaag gggcgatcaa ataagagtca acgacgagga tcgacgtcgt
      961 tgagttgcat tccatcgcaa acgcagtgcc gtccctgaag ggactcgcta gtttttccac
     1021 tttccccagg gcttacgccc tgggctagta ttgtatcgcc cttcggggct tggattttgc
     1081 gcaatcgttt caccgagcga cctcaaaagg agttctcgcg cacgctctaa aggcccgcat
     1141 cacccgccac tgacctttaa cccctaaccc ctgacaccta gcccctgcct taccgattcg
     1201 ccttcagcat ctgctcggcg tgcttcagcg aggtgtccgt caatttcgcg ccgctcatca
     1261 tgcgcgcgat ctcgcgcgtg cgttcgtccg gattcaacgg ccggaccgag gtcttcattc
     1321 tcccgcctga ttcccgcttc tcaatgaggt aatgctggtc ggcgaacgac gcgatctgcg
     1381 gcaggtgggt gatgcacagc acctgcttgg ttcgtgcgag ctgcttcagc ttcttgccca
     1441 ccgcttcggc ggcgcggccg ccgatgccac tgtcaatctc atcgaagacc agcgtccgct
     1501 gtgaacccac cctctcgcca gaagcgggcg agaatggtgg ggcacccctg tccccgcgcg
     1561 cgccattgtg acggccccgg ccggcgctgc cctcctcaac gctggtcttc agcgccagca
     1621 tcacccgcga cagttcaccg cccgacgcga tttcctcgac cgggcccagc ggctctccgg
     1681 gattggcgga gatcgaatac tgcacctggt cgaagccacg ggcgctccag ttcccttctt
     1741 catccgagcc gctgacttcg accttgaagc gcgccttcat ggccagctcg ttgatctcgc
     1801 tttcgaccat cttttccagc ttgcgcgccg cttcgtagcg atggcgggaa agactgcgcg
     1861 ccgcctccag atatttcgct ccggcgtttg ccagttcaat tttcagtgcg tgcaggacct
     1921 cgtccttgtt ctccatctcg ttgagcttac ggtccacttc ctcaccgagc gcgatcacgt
     1981 cgtcgagagt tggtccgtat ttccgcttca agcgatcgag caaggccagc cggtcttcca
     2041 cttcggcgag acgcgcagga gaagcctcga tgccttcggc gtaatcgcgg agctcctgac
     2101 cgatgtcttc cagggtgatg cgcgctgact caagctccgg cagggatggg cggaacttct
     2161 cgtcgaagcg cgataactcc tcaatgtgtt tggtcgcgac acgaatcgaa gcagtcgccg
     2221 acgcgttgct ctcatagagc gcgtcgtacg ccgccatcgc agccgtgaag accttctcgg
     2281 agttagcgag cacgcgcttc tcggcttcga gcttggtgtc ttcgtcgggc tgcacgcgag
     2341 cgtcggtgat ctctttgcgc tgaaagctcc acaaatccaa caggcgcagc cggtcatgct
     2401 cgtcgcgctc caggtcggca atgcgatggc ggacctggtt ccagtcttcg aaggccgcag
     2461 caacggtcgt ggtgtcaact ccggcgaaag catccagaag cgccaggcgc gatgccgcat
     2521 cgaacgccag tacggccaca ttctgtgcat ggatcaccgc cagatgcggc gcaagttgct
     2581 tgaggacagt caccgtcgcc ggctggttat tgacaaagac gcgacccttg ccgccggtgg
     2641 caatctcacg gcgaagaatc agttgcccat cttcagcatt ggaatccagg ccgttggctt
     2701 ccagcaccgc atccagggct ttcgactcgg cctcgaagac cgccgaaacc acggccttgt
     2761 cggtgccatg acgaatcgtg tcggtggagg ctttatcgcc gagaagcaaa gcgagagcgt
     2821 caatgaggat ggattttccg gccccggttt cgccggtcag caggttgaga ccgggcgcga
     2881 actccacggc aacgttatca atgaccgcgt agttttcgac ccgcagttcc agtagcacgt
     2941 gagctctcga ctcgccgaag ttaagcgcag tgtagcagaa agct
//
LOCUS       DASRKK010000819         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_191133, whole genome shotgun sequence.
ACCESSION   DASRKK010000819 DASRKK010000000
VERSION     DASRKK010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191133"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFC15_19590"
     CDS             complement(<1..287)
                     /locus_tag="VFC15_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HZM12417.1"
                     /translation="MAKQILHGEESRQSILRGVNVLADAVKVTLGPKGRNVVIEKKFG
                     SPLITKDGVTVAKEIELKDPLENMGAQMVREVASKTSDVAGDGTTTATVLA"
     gene            complement(387..710)
                     /gene="groES"
                     /locus_tag="VFC15_19595"
     CDS             complement(387..710)
                     /gene="groES"
                     /locus_tag="VFC15_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZM12418.1"
                     /translation="MATKLTPLHDRIVIRRVEEAETSRGGIIIPDSAKDKPQEGEVIS
                     VGRGKSNDKGETFPLDVKAGDRVLFGKYAGTEIKIDGEDFLIMREEEVLGILEGAAKP
                     ALAAK"
     gene            1240..2544
                     /locus_tag="VFC15_19600"
     CDS             1240..2544
                     /locus_tag="VFC15_19600"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM12419.1"
                     /translation="MNRPLPSNRLLPLVAVLGLAMLISPSLIAAQTTSQSDVNLPAAA
                     LQGPVVDPVPGDANIQADQSQAGQMHTAAQVEMEGERVPDPADVPAAVDPTVKSVASP
                     APSMSEAVSAPAVPGTQQPSKYDVTLIGHRNVGTGMNFYSIEREIALGRDLSRQVEES
                     SKLVTDPEINEYVNRIGQNLVRNSDSRVPFTIKVLDSGEVNAFALPGGFFYVDSGLIL
                     AADSEAELAAVMAHEIAHVAARHATKNMTKQQIWNMASIPLMFIGGPAAYAIAEVASI
                     AVPLGFLKFSRDAEREADMLGLQYDYAAGYDPQAFVQFFEKLKVEEKKKHSNIAKMFS
                     THPMNSERITAAQNEIRTYLPDRDSYVVDTSEFEAMKARLIGLEGGLKITKAENANRP
                     VLRKPAQSQDASDSEPSATPQPGSTDDTDDRPTLKKAPATTN"
     gene            2622..>2882
                     /locus_tag="VFC15_19605"
     CDS             2622..>2882
                     /locus_tag="VFC15_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12420.1"
                     /translation="MRNLRPLGWLLCMLLVSLLVLASDKQAGAQQSSNLRFTVVKDDN
                     NKPVRNASVVLHPVGKSGKQSRGGFQLKTDSEGKTETDGIPYG"
BASE COUNT          593 a          754 c          884 g          651 t
ORIGIN      
        1 tgggccagaa cggtcgcggt ggtggtgcca tcaccggcga cgtccgaggt tttggaagcg
       61 acttcgcgga ccatctgggc gcccatgttc tcgagcgggt ccttcagttc gatttccttg
      121 gctacggtga cgccgtcctt ggtaatgagc ggcgaaccga atttcttttc aattaccaca
      181 ttgcggccct tggggccgag tgtgactttt acggcgtcgg cgagaacgtt tacgccgcgg
      241 aggatcgact gccgtgactc ctcgccatgc agaatctgct ttgccattgt ttctatctcc
      301 ttgaaattca gtggtcagtg gatagtggct agtggctaga aaaaccagcg tagccgcggc
      361 tcactgacgg ttcttgttga acaaacctat ttagcggcga gggccggctt ggcggcgcct
      421 tcgaggatgc cgaggacttc ttcctcacgc atgatcagga agtcttcgcc gtcaatcttg
      481 atttcggtgc cggcatattt gccgaacaac acgcgatcgc ctgccttcac gtcgaggggg
      541 aaggtttcac ccttgtcgtt gctcttgcca cggccgacgg agatcacttc gccttcctgc
      601 ggcttgtctt tggcactgtc agggatgatg atgccaccgc gggaggtttc ggcctcttct
      661 acgcgacgaa tcacgattcg gtcatgcagg ggcgttagtt tagtagccat tcttcagact
      721 cctttttccc gcctaggcgg gtcgattcgg attggattgc gataccagtt ggccggcgtt
      781 ggcgcgtgtg ttctgcgttg gacccggcat gttcatgaac tttggagttt ttgtcccgag
      841 cgctaaagct agaggtaggg attgacgtcc ccgcggttgc tagcactcag gtgcatcgag
      901 tgctaatgta ccccagacgt gagaggaaag tcaagcagat gtgagtgaaa gtcgctcaaa
      961 tttcaaaatt cacgaaaatg gcacatctgg gtgttgcctt ttggcgcctg aggagtagta
     1021 tttcaccaat tagcccgccg cgaggcaggc gactgatgct gggcgaacgc gggtatccga
     1081 cttcataagt tttgcacctt aggcggcttc tgttttggga atatgttaat caaccccaaa
     1141 gcgggccacg tgcttggcgc ttatagcaag tttctgtttg caaccttcga ctcaaccaac
     1201 ataattggaa tcgcggttgt ttgcgtgcag gtactcccta tgaatcggcc cctcccttcg
     1261 aatcgcttgt tgccactggt cgctgtgctg ggtttggcga tgttgatttc gccgagcttg
     1321 attgcggcgc aaaccacctc tcaatccgat gtgaatttgc cggcagcggc tttacagggc
     1381 cccgtagttg acccggtccc cggagatgcc aatatccagg cagatcaaag tcaggctggc
     1441 cagatgcaca cggccgcgca agtggagatg gaaggcgagc gtgtgcctga ccctgcggat
     1501 gttccggccg cagtcgatcc cacggtgaag tcggttgctt ctccggcacc ctctatgtct
     1561 gaggcggttt ctgctcctgc cgtcccgggc acgcagcagc cgtcgaagta cgatgtgacg
     1621 cttatcggtc accgcaatgt cgggacggga atgaatttct attccatcga gcgagagatc
     1681 gcgctgggcc gtgatctgtc acggcaggtc gaagaaagct cgaagctggt gaccgatccg
     1741 gagattaatg agtacgtaaa ccggatcgga cagaacctgg tgcgcaattc ggattcgcgg
     1801 gtgccgttca cgatcaaggt actggatagc ggcgaggtaa acgcgtttgc gttgcccggc
     1861 ggattctttt atgtggacag cgggctgatc ctggcggccg atagcgaggc cgaactggcg
     1921 gcggtgatgg cgcatgaaat cgctcatgtg gcggcacgcc atgccaccaa gaacatgacc
     1981 aagcagcaga tctggaacat ggcatcgatc ccgctgatgt ttatcggcgg cccggcagcg
     2041 tatgccatag cggaagtcgc gagcatcgcg gtgccattag gtttcctgaa gttctcgcga
     2101 gatgccgaac gcgaggctga catgctcggc ctgcaatatg actacgccgc tgggtatgat
     2161 ccacaggcat ttgtccagtt cttcgagaag cttaaggttg aagagaagaa gaagcacagc
     2221 aatatcgcca agatgttctc gacgcatccg atgaactcag agcggattac ggcggcgcag
     2281 aacgagatcc ggacttacct gcctgaccgt gacagctacg tggtggacac cagcgaattc
     2341 gaggcgatga aagcgaggct gattgggcta gaaggtgggt tgaagattac aaaggcggag
     2401 aatgcgaatc ggcctgtgct gcggaaacct gcccagtcgc aggacgcgag cgattcggag
     2461 ccgtctgcga cgccacagcc gggttcgacg gatgataccg atgaccgtcc gacgctgaag
     2521 aaggcccctg ccactacgaa ctagacgtct gacaatgtgc gaaaggcagg gtgcgggtta
     2581 gcagtttgca gccttcgctg ccggatgcct agaatactcc catgagaaat ctccgcccgt
     2641 tgggctggct gttgtgcatg ctgttggttt cgctcttggt gcttgcgagt gacaagcagg
     2701 ctggagcgca gcagtcctcg aatctgcggt tcaccgtggt gaaggacgac aacaacaagc
     2761 ctgtccggaa tgcgagcgtc gtgctgcatc cggtcgggaa gagcggtaaa caatcccggg
     2821 gcggatttca gctgaagacc gatagcgaag ggaaaacgga gacggacggc ataccctacg
     2881 ga
//
LOCUS       DASRKK010000820         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_972932, whole genome shotgun sequence.
ACCESSION   DASRKK010000820 DASRKK010000000
VERSION     DASRKK010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972932"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFC15_19610"
     CDS             <1..1605
                     /locus_tag="VFC15_19610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM12421.1"
                     /translation="VYVVLGATHPNLVRDQGEAYRESLIARVRELGVEDHVLFLNQFV
                     DQATLLEFISMSDVYVTPYLNEAQMTSGTLAYSFGLGKPVVSTPYWHARELLADGRGI
                     LVPFGDARAIGSAIAELLTDDTRRQKMRKRAFSTSRSMTWERTAGRYVSAFDSARQGH
                     WLKVIARPERSTAAANNLSAPEMQIGHFLSMCDDTGLFQHAVHSIPDRSHGYCVDDNA
                     RALLLACALNNPGEQRLSEVLTARFAAFVQHAWNPDTKRFRNFLGFNRNWLEDSGSED
                     SHGRTLWALGECARRDADPSRRRWAASLFAEALPTAEAFHSPRAWAFTLLGLGAYCAV
                     VPDDLHAQEARYLLADQLMASLASVETSGWVWFEEGLAYDNARMPQALIVIGMATQTP
                     AYINAGLRSLRWLMMQQTTPGGHFRPVGTAGFGEQRQHPHAFDQQPLEATATIAACLA
                     AWRADGDAEWKVVAARVFGWFHGSNDLSVSLVDLHTGSCRDGLHPDRANENRGGESVV
                     SYLLSLAEIRQLTRTNAIPSLAARVA"
     gene            complement(1806..2015)
                     /locus_tag="VFC15_19615"
     CDS             complement(1806..2015)
                     /locus_tag="VFC15_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12422.1"
                     /translation="MSNRVNVEIDSIHSRAICKEIGERLRISVSRESPELPPRMRTQL
                     DQLRKLEASDSPSIVPSIFGFGDKQ"
BASE COUNT          568 a          791 c          802 g          573 t
ORIGIN      
        1 gtgtatgtcg ttctcggtgc gacgcatccc aatctggtcc gggatcaggg cgaagcctat
       61 cgcgagagtc tgatagcgcg cgtgcgcgaa ctcggcgtcg aggatcacgt cttatttctt
      121 aaccagttcg tcgatcaggc cacgctgctc gaattcattt cgatgtccga cgtctatgtc
      181 acgccttatc tcaatgaggc gcagatgacc tcaggcacct tggcctatag ctttggacta
      241 gggaagccgg tggtctcgac tccctattgg cacgcgcggg aattgctggc ggacggacgc
      301 ggcatccttg tgcctttcgg cgacgcacgg gcgatcggca gcgcgattgc ggaattgctc
      361 accgacgaca cccgccgaca gaaaatgcgc aagcgcgcct tttcaaccag ccgatcgatg
      421 acgtgggagc gcaccgctgg gcgatacgtg tctgctttcg acagcgcgcg gcaaggacac
      481 tggctcaagg tcattgcgcg ccccgagaga agcacggctg cagcaaacaa tctttctgcg
      541 cctgagatgc agatcggtca tttcttatcg atgtgtgacg acaccggctt gttccagcac
      601 gcggtgcatt cgatacccga tcgctcccac ggttattgcg ttgatgacaa cgcccgcgca
      661 ttgctgttgg cctgcgcgct caacaatccg ggcgagcagc gcctctccga agtcttgacg
      721 gcccgctttg ccgccttcgt gcagcatgcg tggaatcccg acaccaagcg atttcgcaac
      781 ttcttgggtt tcaatcgaaa ctggcttgaa gacagcggtt cggaagacag tcacgggcgg
      841 acgctttggg cgttgggtga gtgcgcccgc agagacgccg atccatcacg gcgtcggtgg
      901 gctgcctcct tgtttgctga ggcattgcct acggcggagg cctttcactc gccacgcgcg
      961 tgggcgttca cgttgctggg tttaggcgcc tactgcgctg tagtcccgga tgatcttcac
     1021 gctcaggaag cccgatatct tctcgccgac caactgatgg cgagcctggc gtcggtcgag
     1081 acgtcgggct gggtctggtt tgaggaaggg ctggcctacg ataatgcgcg catgccacag
     1141 gccttgatag tgatagggat ggcaacgcaa acgccagcct acatcaacgc tggattgcga
     1201 tccctgcgct ggctgatgat gcaacaaaca acaccaggag gtcatttccg tccggtcggc
     1261 acggccggtt tcggcgaaca gcgacaacat ccccacgcct tcgaccagca acccctggaa
     1321 gccacggcaa cgatcgcagc ttgcctcgcg gcttggcgag cggacggcga tgcagaatgg
     1381 aaagtcgtcg ctgcgcgtgt tttcggttgg tttcacggca gtaacgatct gtccgtgtcg
     1441 ctggtcgatc tgcacacggg aagttgccgc gacggacttc accctgaccg tgccaacgaa
     1501 aatcgcggtg gcgagtccgt cgtttcctat ctcctcagtc ttgcggaaat acgtcaactt
     1561 actcgcacca acgccatccc tagcctggcg gcccgcgtcg cttgaccatg tacttggcga
     1621 gggcgcctat ggattccagg cgattactga catcggtgcc gcccttcagc aggcggccga
     1681 gaactccgac accgacgcgt tgcgcaagtg ggtggatgaa ttatccagct acctggatgg
     1741 tgtcgagagc atttccaatt agcccgtcac tcaatcccga aaaccaatcc gttctgcgac
     1801 gcaacttact gtttgtcgcc aaaaccgaag attgaaggga caatcgacgg cgagtcggat
     1861 gcttccagtt ttcgtagctg atccagttgc gttcgcatgc gtgggggcag ttccggcgat
     1921 tctcgcgaca cggaaatgcg cagcctttct ccgatctcct tgcagatagc acggctgtgg
     1981 atgctgtcga tttcgacgtt gacacggttc gacatgacta atccagtcta ttcatttgaa
     2041 tttaacaaca ttggaccaag tcgaaaacgg caaacttgtt tcgacggatc aatcccaaag
     2101 acgtgtacgg gagaaaatta aattccagac aggtggaacg aagccgccct acagaataac
     2161 tgttcgctga ccttctgaaa aagcatgggt ccaaccgcac tgatggggtt ccaaccgcac
     2221 tgaaggactg ctgaaaagaa gacggctagt catcgcgcaa catcttgctc cagaggtgtc
     2281 gatgtcgcag ttatgggcgc cgatcgggtt cagggcgacg ccctaaattc ggaagcatcg
     2341 ccgcttgcca ttccggaatt tgccgcccga aagaagaggg aggcggccgg acccccgcca
     2401 tattcccaag ttcgaggaaa ctcacgcaga gactgggttc gatcgggtac gcaggaggtc
     2461 ggcgcccacg gcagagagag ctggcaccgg gtacaggcaa tctggaaggc gatcgacgac
     2521 tatgccgagc ttgaaaccgg caaccgagag tacttttgga acaggccgca cagcatcggc
     2581 tgattaggac gtgcgcggcg gccttaactc gctcgctagc cagcctacgg cgtcggtctt
     2641 catcgggtct acgggaaaag aaactttctc gatgcggtgg caggtcgagc actcgaacgt
     2701 gcgctcttcg aagcctcttt cacccggcga gatt
//
LOCUS       DASRKK010000821         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_287869, whole genome shotgun sequence.
ACCESSION   DASRKK010000821 DASRKK010000000
VERSION     DASRKK010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287869"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFC15_19620"
     CDS             <1..736
                     /locus_tag="VFC15_19620"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM12423.1"
                     /translation="MRKFLQLGFLGLLVASQMHAAEIANLRNGFSIRHERHEVDGDTT
                     RLYMSAEPSSGYVDVPTAQIENFEPAPPEPQAATSQGQKPVDLNVIVSAASTRSQVDA
                     DFIASVIRAESGNNPHAISRKGAQGLMQLMPGTASKLGVKNTFDPAENVDGGVRYLRE
                     LLLLYNNDMVKALAAYNAGPQRVQQYKGVPPYRETHAYVARVINDYNRKKLAQRKQRQ
                     KPPVRSAAAGKPEIAQSATGSAADTE"
     gene            852..1886
                     /locus_tag="VFC15_19625"
     CDS             852..1886
                     /locus_tag="VFC15_19625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM12424.1"
                     /translation="MNKKRTLIFGIVLIALCLLIYLQIRAWKTFDWHMFWNRTEHIDW
                     LYAFYGLALTYFAYVLRAVRWRIFLKPVRKTTTARLLAPQFVGFAALALLGRAGEMVR
                     PYIIAKKERLTFTSQIAVWGVERIFDMGAFAVLLALSFLSPDLRALPFYHGLRNAAFA
                     LIVLVGALCVGAFVVRRWGDRVGQFLHDLFAGFAPKFAHHLREKVRSFSDGLATTHDL
                     SSALQLTGISILMWVLIAVAYYAVTHAYPGELRSLTLPHQVVLMGASMVGSMLQLPAV
                     GGGSQLATISMLSSGFGIEKELAVSCGILLWAITFMSVIPMGLLLARREHVSIRAVAE
                     AEEKEAALLE"
BASE COUNT          504 a          775 c          780 g          527 t
ORIGIN      
        1 aatgcgtaag ttcttacaac tcggtttctt gggcttgctg gtggcgtcgc agatgcacgc
       61 cgcggaaatt gcaaacttgc gcaacggttt tagcattcgc cacgagcgtc atgaggtgga
      121 cggcgatacc acgcgcctgt atatgagcgc ggagccgagc agtggatacg tcgacgtgcc
      181 caccgcccag atcgagaact ttgagccggc gccgccggag ccgcaagcgg ccacatcgca
      241 agggcagaag cccgtggatt taaacgtgat cgtcagcgca gccagcacgc gcagccaggt
      301 ggacgccgac ttcatcgcca gcgtgatccg tgcggagagc ggcaacaatc cgcatgcaat
      361 ttcgcgcaaa ggcgcgcaag gactgatgca actcatgcct ggtaccgcca gcaagctggg
      421 ggtgaagaac accttcgatc cggcggagaa tgtcgacggc ggggtgcgct acctacgcga
      481 actgctgctg ctttacaaca acgacatggt caaagcgctg gccgcgtaca atgccggtcc
      541 gcaacgcgta cagcagtaca agggcgtgcc gccctaccgc gagacccatg cttacgtggc
      601 gcgagtcatc aacgactaca accgcaagaa gctggcccag cgcaagcagc gtcagaagcc
      661 accggtgcga agcgcggccg cgggcaagcc agagattgcg caaagcgcga caggttctgc
      721 ggcggatacc gaatagctcc gggtttgacc ccactcattc ggtcgtatac cttgacggct
      781 gagtctcagt cgcaggcctg cgtccaacct cacgagaaag caacgcgtgc cggtggccac
      841 tgatttcttc catgaacaaa aaacgcacgc ttatcttcgg catagtcctg atcgccttgt
      901 gcctgctgat ttacctgcag attcgcgcct ggaagacgtt cgactggcac atgttctgga
      961 accgcacgga acacattgac tggctttacg ctttctacgg cctcgcgctg acctacttcg
     1021 cctacgtact gcgtgccgta cggtggcgca tcttcctgaa gccagttcgc aagaccacca
     1081 cggcccgtct gctggcgcca caattcgtcg gcttcgccgc cctggcgctg ctgggccggg
     1141 cgggtgagat ggttcgaccc tacatcatcg ccaagaaaga gcgactcacc ttcacctcac
     1201 agatcgcggt ctggggagtg gagcgcatct tcgatatggg ggcctttgca gtgcttctgg
     1261 cgctgagctt tttgtcgccg gacctgcggg cgcttccctt ctatcacggc ttgcgcaatg
     1321 ctgcgttcgc gctgatcgtg ctggtggggg cgttgtgtgt aggggccttt gtggtgcggc
     1381 gctggggcga ccgagtgggc cagtttctgc acgatctgtt cgccggcttt gcgccaaagt
     1441 tcgcgcacca cttgcgcgag aaagtgcgct ccttcagcga cggcctggcg accacccatg
     1501 accttagctc agccctgcaa ttgaccggga tctccatttt aatgtgggtg ctgatcgccg
     1561 tcgcttacta cgcggtgacg cacgcctatc ccggcgaact tcgcagtctc acgctgcctc
     1621 atcaggtggt gctgatgggc gccagcatgg tgggctccat gctgcaactg ccggccgtcg
     1681 gtggcggctc gcaactggcc accatcagca tgcttagctc ggggttcggc atcgagaagg
     1741 aacttgccgt aagctgtggc atcctgctat gggccattac cttcatgtcg gtcattccga
     1801 tgggattgct gctggcgcgc cgcgaacatg tttcgattcg agcggtggcc gaggcggagg
     1861 aaaaagaagc ggcgctcctg gagtgaaacg gcagcgcgct tcggcacgcc cgcgtatggc
     1921 agcaggcgcg catcttcgcc cggaacggtt gcagaattgg tgtatccggg tagagcaggc
     1981 cttttgaagc agtttcacat ctggtgtata ggcgatgggt ggagcgcgcc tttcaggcgt
     2041 gcataacggc cgtcaggtcc tggatcgcgc tttagagcag attctgcgtt ggtgtatccg
     2101 ggtggagcac cgcttcagcg gtgcatttga gagttgcctc tgaatttggc tttagccgct
     2161 gaggtaacca gaactcttac ctaagcggct aaagcgtcgc cctttttgca gcctttggta
     2221 tgcacgcccg aaaggcgtgc tccacccaac accggataca gcaactccgg aatcgcttta
     2281 gcgcctgagg taatgtttcc gtcccctcag tggctaaagc cagctcattt ccgaatccta
     2341 agctgcaccg ctgaagcggt gctccaccct atacaccaag tgtgaaactg ccctaggcct
     2401 gcgtccagcg caaaaaagga accgctccag ccgctgaggt cgtcgacctc agcggctgaa
     2461 gcaggaattg tgcggaccct taacgcgggt ctgaatgaag gcccgcccta cccgagccac
     2521 agccattctg aaactgctct aggctccgca gcggacaaga ccagggaagt tttctggtcc
     2581 ggagct
//
LOCUS       DASRKK010000822        17073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_387451, whole genome shotgun sequence.
ACCESSION   DASRKK010000822 DASRKK010000000
VERSION     DASRKK010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387451"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..336)
                     /locus_tag="VFC15_19630"
     CDS             complement(85..336)
                     /locus_tag="VFC15_19630"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZM12425.1"
                     /translation="MSSTEKTISFRADADKIDELDALAAAQDRSRSYLINEAIRNYVE
                     LHAYQDALVREGLAEMRQGRVVSHEEVVRRLKKTGRASE"
     gene            complement(1025..1633)
                     /locus_tag="VFC15_19635"
     CDS             complement(1025..1633)
                     /locus_tag="VFC15_19635"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HZM12426.1"
                     /translation="MRRSLAALVICSSLAVQALAQQPQASALRVSDSPTFTADGKLEF
                     PANYREWIYLTSGLNMSYAPRMAGMAGHDMFDNVFVNPTAYKVFLDTGTWPDQTMLVL
                     EVRGAGSNASINKSGHFQTTDEMGHEVHLKDKRVPGGWAFYGFDETKPTTPFPHEMDC
                     YSCHEQHAAVDTTFVQFYPTLIKLAEQKGTLSAGYKKDEAEK"
     gene            1724..2542
                     /locus_tag="VFC15_19640"
     CDS             1724..2542
                     /locus_tag="VFC15_19640"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZM12427.1"
                     /translation="MRKITSLLRGIHYNIRMDSFIIAGRQFRSRLIVGTGKYKSGQET
                     ARAIEASGAKMVTVAVRRVNLDRSKESLLDFIDPKKYFLLPNTAGCYTADEAIRTARL
                     GREVGLSDWVKIEVIGDQATLFPDVQATIEATRVLVKEGFTVLPYTSDDIVVAKRLID
                     AGASAVMPLGAPIGSGLGIQNQPSMRILRELITEVPLIVDAGVGTASDAAIAMELGMD
                     GVLMNTGIAGAQDPVLMAEAMKNAVIAGRQAYLSGRMPKKLYATASSPLEGVVR"
     gene            complement(2668..3909)
                     /gene="lhgO"
                     /locus_tag="VFC15_19645"
     CDS             complement(2668..3909)
                     /gene="lhgO"
                     /locus_tag="VFC15_19645"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HZM12428.1"
                     /translation="MADRQADVIIIGAGIVGLATALEYTRRFPGRRLVVVEKEDRVAA
                     HQTGHNSGVIHSGLYYKTGSLKARNCVAGCASMKRFCQEYSIPYEECGKLVVATSPEE
                     VPRLEQLHQRGIANGVERTRMLSRDEFREIEPHCDGICALLVPSTGIVDYKDVAEKYA
                     ELIEHAGGEIVLRAKVVGLRDDGDANVVETTAGMFRASYVINCAGLYSDAITRLAGCN
                     TDLEIVPFRGEYYEVKPERRYLIRNPIYPVPDPRFPFLGVHFTRHVDRSVEAGPNALL
                     AFRREGYTGTSVDLTEAVATLSYVGFWNMARKFWRKGLEEQYRAWVKSAFTRALQQMV
                     PELTEADLIPGGSGVRAQAVDKNGNLVDDFHFIHSQRTIHVCNVPSPAATASLEIGRE
                     VVDMLVNCFDLASDPVPSIKV"
     gene            4039..4992
                     /locus_tag="VFC15_19650"
     CDS             4039..4992
                     /locus_tag="VFC15_19650"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZM12429.1"
                     /translation="MRVLVTGGAGFLGSHLCDAMLAEEHSIVCADNLLTGRMENLAHL
                     GRESRFEFLQQDVCEAFDPGKIDYLFHFASPASPVDYLKHGIETLRVGSAGTINCLEI
                     AKKYNAKFLLASTSECYGDPLEHPQKESYWGHVNSIGPRSVYDEAKRFAEAATMAYHR
                     YHAVDTRIVRIFNTYGPRLQIDDGRVISNFMRQALRGEDLTVYGDGMQTRSFCCVSDE
                     IAGILALSKSEEHLPVNIGNPTEFTMLKCAEVVLEVTGSASKIRFELLPQDDPKQRRP
                     DITKARTLLGWEPKIDLRTGLQLSLNYFREAVLANSSAACR"
     gene            5054..5863
                     /locus_tag="VFC15_19655"
     CDS             5054..5863
                     /locus_tag="VFC15_19655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM12430.1"
                     /translation="MDAQVELDELHRDSESWWYTTRRKLLREAALQAVHGKREARVLD
                     LGCTAQLQFDDSTMYRVCNVHSSLRTLAFRQIEGDDNLVCSRAEELAFTSNSFDAIVA
                     GDVLQSLDDDVSALREMRRVLKEGGLLCLTAPAYSFLWGEDDEARGNHRRYTVSELRR
                     KLTTSGFEIHRASYFVASAFLPLAVGRAAMDIFRKSITRHRHYPHPSQLANAAMAALL
                     DGERILLHYINFPFGTRVVCWALKPALVAETVTVPAWERKWAQHPLAQGSG"
     gene            5881..6756
                     /locus_tag="VFC15_19660"
     CDS             5881..6756
                     /locus_tag="VFC15_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZM12431.1"
                     /translation="MAVLRSIFRNTGTTLEMIMWEHSVLTLPFGLTGAVLAARGIPNL
                     HTSIWIILALVAARSAAMAFNRLVDADIDAENPRTKIRALPAGKLSKQFVLGFVVVTS
                     AILMFAAWKLNPLSFYLSPVALAIIFFYSFTKRFTRWAHVVLGLAMGIAPAAAWIAVR
                     GSFDPRILLLTGAVVFWGAGFDILYSCQDYEFDRDANIYSVPMAFGIRNSLMIARLFH
                     VFTVLLLAGMAVAFGLGKIAMVGVVMVALLLIYEHSLVKHDDLSRVNAAFFTMNGFIS
                     MGFLFFIAADLLVAR"
     gene            6844..8001
                     /gene="mqnE"
                     /locus_tag="VFC15_19665"
     CDS             6844..8001
                     /gene="mqnE"
                     /locus_tag="VFC15_19665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HZM12432.1"
                     /translation="MATVEQTLAGRKQHPFQTDDPRLTSIAEKVLAQERLSEEDAFAL
                     YRSGDILAIGWLANHVRERVHGDIAYFNVNRHINYTNVCVAACRLCAFGRKKDTPGAY
                     TMALEEVWNTAATGYTEAVTEFHIVGGLHADLPFQYYPDMIAGLKQRFPQVHLKGFTM
                     VEISFFAKIGKMTPREVLVRLKEAGLDSMPGGGAEIFSDRVRHIICDHKIDGDDWLDI
                     ARTAHEVGLKSNATMLYGHIEHDEDRVDHLLKLRALQDDTHGFQTFIPLAFHPDNTPL
                     QHLPKTTGMLDLKQIAVSRLVLDNFPHIKSYWQMVTPKVAQISLRFGADDIDGTVVEE
                     KIYHDAGATTPQGLSRQDLIRLITEAGRVPFERDTLYRPVTRTETTVTVAV"
     gene            complement(8007..9236)
                     /locus_tag="VFC15_19670"
     CDS             complement(8007..9236)
                     /locus_tag="VFC15_19670"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HZM12433.1"
                     /translation="MKVTRRGFIATTALGSASIALDLHAAQQAPGYKPPASGPKPKSP
                     VIICAANGYNYLDRGYAVLISGGDTLDAVNQVITGPEDDPNDDSVGLGGLPNEDCVVE
                     LDACCMHGPTRMAGSVAGVHDIKNVSLLAKTVMEHTGHVMLVGEGASKFGLLMGFPKE
                     NLLTERSRKTWQLWRETMSTDDWWGPGLASPNFKFPDTGGDTTELQQQLIEKMEKLAA
                     RIGIAPEFRMAAIERVLKPPTGTINCSSINEKGEMSSATTTSGLAWKIAGRAGDSPII
                     GAGCYCDQDVGAAGATGNGEENIKVCGGHTIVENMRHGMAPEEAGLDALKRIVRNYNG
                     DMHKLQYMDMTYYILRNDGAYAGVSLWAGPPDHQRRYAVHDDKGKRYAKATPLFQGAS
                     LGWPPMPGGKRLKPEQK"
     gene            complement(9315..10286)
                     /locus_tag="VFC15_19675"
     CDS             complement(9315..10286)
                     /locus_tag="VFC15_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12434.1"
                     /translation="MRYLLALLALSTTMFAQALKPLQADDAVRIAEFYRLATQIQDRI
                     WLGWSKTPALLLLVTSDTEYLTHFPTPPKDFQKIGDDIYARPRQFPPNMQATFPAFGF
                     PPVIVIGEPSNTQCKTSTPWLFVVMHEHFHQLQWAQPDYLKAIADLGLSKGDATGMWV
                     LNYPFPYDNPEFAESFGYLRDSLLHALNEVDKQKFDQLSKQYLKDRKQFFAQLLPGDH
                     KYFSFQLWQEGVARYTQVKAAEAAEDYQPSSAYAALPDFTPFNAYAVIARSRTLDELK
                     HIDIAQSKREVFYSFGAAEALLLDRINPSWKDEYFKHLLTTDPLFPR"
     gene            complement(10390..11559)
                     /locus_tag="VFC15_19680"
     CDS             complement(10390..11559)
                     /locus_tag="VFC15_19680"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12435.1"
                     /translation="MITRDDIRELANFLSPEGCAVTFYYQPAAPTNRSHRDETILVKD
                     LVNNSLRGAEKKGSKQKSAVADLQRILEMTEMLRNNGRHGKAIFACAAKGIWREFDLP
                     AKLPKTNLILNQRFHLKPLAAIFDGMQHACVVLVDRTKARVFEIADGTILEKLDFINE
                     LTRRGRSDGWGGYDAGHAERRVMNEAMQHFKVVSDAVSGHIEHGGCEKLLIGCHDDVW
                     SEFLPHLHSYSRQRLVGHFRIDPKSATPEQVRQAALELLEQRDEERKQAMITEVIGEA
                     HRNGRGAIGLRRVLRSLEAGEVQTLMLAASFSAPGVKCYHCGHMDYHLAPDCLMCGKP
                     NTELEDLGDAIVGHAIRNGLEIVYIGDDEQFDKIGRIAAQLRFRADQNTPAKIAV"
     gene            complement(11622..12662)
                     /locus_tag="VFC15_19685"
     CDS             complement(11622..12662)
                     /locus_tag="VFC15_19685"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HZM12436.1"
                     /translation="MNVHFSYKISKTADLEKQTQHQLEKLSKHLQVFRPELVHVKGIV
                     EENSAREGFVVSLNLRLPSGQMAAQEKSATAAAAIRAAFDALIEQLKRHKQVLRSKHK
                     WVRRRDAGRTGTGNVPFEDTLAVVRPETVSSLDIASYIDINLPRLQRFVERQINYRES
                     LGELLPDQVAPEDVVAEAIATALGDEMEKPERIKIEPWLYRLSSEAIDRMSASDDGGG
                     MVPLERGHGTQNVLASDEVRLQFHQPDERLLEEDTIPDGHAGNPEEMTARGELIAVVQ
                     LALRDAKRSDREVFILYAVEGFAEEEIATITNHSLEEVHASIRKARQHLQRALPIQDL
                     FKTKHVEYTHTT"
     gene            complement(12946..13992)
                     /gene="glpX"
                     /locus_tag="VFC15_19690"
     CDS             complement(12946..13992)
                     /gene="glpX"
                     /locus_tag="VFC15_19690"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZM12437.1"
                     /translation="MATKVHAEPKPAIDINHYTPRTNFEADLALEFLRVVEESAIASA
                     KTMGQGKRELADQMATESMRGVMDSVPMDGTIVIGEGERDQAPMLYIGEKVGKHDGGQ
                     YPQVDIAVDPLEGTNLCATGSPGAITVLAASENGGLLHAPDCYMEKIVVGPSCKGVVD
                     LEAPVGHNLKVIAKVLGRKVEDLVIIILERPRHEKLIEDIRKAGARIRLISDGDLSPG
                     IAAGMLGSGVHAVMGIGGAPEGVITAAAIRCLNGYMVGRLTGYTPEQADRMSDMGIKD
                     PKRIYTADELAPGKNIVFAATGVTDGPLLKGVRFFGDGVRTSSLVMKLQNRKVRFIES
                     IHVNPSPDAKVRIP"
     gene            14347..14658
                     /locus_tag="VFC15_19695"
     CDS             14347..14658
                     /locus_tag="VFC15_19695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM12438.1"
                     /translation="MNTTGLPIIERMPGWKGRYFHSANMTFAHYEFSRGSSIHEHFHP
                     EEEVYEVIDGELEITIDGVSQTARAGLVAIVPSNPRHSVKALTDGRAIIVDHPARPEF
                     G"
     gene            14713..15150
                     /locus_tag="VFC15_19700"
     CDS             14713..15150
                     /locus_tag="VFC15_19700"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM12439.1"
                     /translation="MTPAIQQSMEFDVSPETLYELYIDSKQHSLATGAPAKLGRRTGD
                     AFTAFGGQLKGRNLLVVPKQMVVQTWRAEGWKKADPDSILVIRFSKTKNGARVDLVHV
                     DVPEYDHQGVTDGWRHYYWEPWRVYLEAVGLKQKAGARSKRKG"
     gene            15295..16122
                     /locus_tag="VFC15_19705"
     CDS             15295..16122
                     /locus_tag="VFC15_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12440.1"
                     /translation="MRELLRFWAKHLLVVVLACGIIPLVASAADETSLTKDQITEFLQ
                     TAEVIKSKPSSKGVTHPSRLTLSNGTITHDASFQTIDEHSELKQLESGHREYNFVDSY
                     KFNIAAYRLSELLGIDNMLPVYVERKWQGKTGSLSWWLPVQMDEADRMAKKIVPPDIH
                     AWNEQMNRIRVFDELIYDTDPNLTNVLIGEDWTVWRIDFTRAFRTYKDLRLPQDLVKC
                     DRQLFEKLKALKGNELADKTKNYLTKDEVNAVMARRDKIVAIFQGLIAEKGEKEVLY"
     gene            complement(16573..16797)
                     /locus_tag="VFC15_19710"
                     /pseudo
     CDS             complement(16573..16797)
                     /locus_tag="VFC15_19710"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3440 a         4822 c         5086 g         3725 t
ORIGIN      
        1 ggctagcgac ctcctcactg ttggagagcg cgatgtaatc gtgggcctgg tccagttgcc
       61 gcgtcgcctg ctcggtccac tcaatcattc cgaggcgcgc ccggtcttct tcagacgcct
      121 caccacttcc tcatgactga ccacccggcc ctgtcgcatc tccgccagcc cctcgcgcac
      181 cagtgcgtcc tgataggcgt gcagctcaac gtaatttcgg atcgcttcgt tgatcagata
      241 gctgcgcgag cggtcctgcg ccgccgccag cgcgtcgagt tcgtcgatct tgtccgcgtc
      301 cgcccgaaag ctgatggttt tttccgtgct gctcataatg aaggaacaat agcatgtatt
      361 atcctgtatt acaagcctcc gtggtggccc aaatattccg tggcccgtat ttgccggcgc
      421 tgctcgctcg ccagtacgat cgagaacaga acccactgga gaactcgcgt tgatcacttt
      481 gcggtgaaac caacctcgtt ccaccaacca tcgatacggt cgcttcttcg gccacgattg
      541 atgtcccaaa ttgggactgc acaggttact ttcgttccgc cgcaaaccca aatcttcttg
      601 cttcccgctc gctcgctcct ctataccttt accgaggatc gagggatttg tgtgccctca
      661 cctcgcggcc caggccgccg ctgcccgcgg ctctttgaca actcgccgtc gtcactcttc
      721 ctccacctca gttttcgacc agtttcaccg tgaccgacct actgtcgtag ctatgtataa
      781 cagtagactg ttattttctg ttacctgcta actcgcttat tctaaaaata atattgtata
      841 cagatgttgt gttgtagtta tgtataacaa taaaaggaat gaaagtggca acgtcgttat
      901 cgctcagtct gccctgagcg cagtcgaagg gttcggccgt cttcctcggc ggcaacggca
      961 tcgccgacga ctacctcatc ttccgccaca tggcccgagg ttactggtcg cgaagtgtct
     1021 tctgttattt ttcagcttca tccttcttgt atccagcgct caacgtgcct ttctgttcgg
     1081 ccagcttgat cagcgtggga tagaactgca cgaacgttgt gtccactgcg gcatgctgtt
     1141 cgtggcaact gtagcaatcc atctcgtggg gaaacggcgt ggtgggtttg gtttcgtcga
     1201 agccgtagaa ggcccatccg ccggggacgc gtttgtcttt gaggtgaact tcgtgtccca
     1261 tttcgtcggt ggtctggaag tgccccgact tgttgatgga ggcgttgctg ccggcaccgc
     1321 gcacctcgag caccagcatg gtctggtcgg gccaggttcc agtgtcaagg aacaccttgt
     1381 acgccgttgg gttgacgaac acgttgtcga acatgtcatg gccggccatg cccgccatcc
     1441 gcggagcgta gctcatgttg aggccgctgg ttagataaat ccactcgcgg taatttgccg
     1501 ggaactccag ctttccgtcg gcggtgaagg ttggtgagtc cgagacgcgc agcgcggaag
     1561 cttgtggctg ctgcgctaat gcctgtaccg ccaggctgga acaaatgacc aatgccgcga
     1621 gtgatcgcct catacccctc cgaagaaccg acgccatcgt tttgcgataa agtccgatga
     1681 tcatacgacg aagctacggg gttgcgaaac cccatcggat gaagtgcgta aaatcacatc
     1741 cctgctgcga ggcattcatt acaatatccg catggactca ttcatcattg caggtagaca
     1801 gtttcgctcg cgcctgatcg tgggcacggg caagtacaag tcggggcagg agacggcgcg
     1861 cgccatcgag gcctcgggcg cgaagatggt cacggttgcg gtgcgccgcg tgaacctcga
     1921 ccgcagcaaa gaatcgctgc tcgacttcat cgatcccaaa aagtattttc tgctgcccaa
     1981 caccgcaggc tgttacaccg ctgacgaggc catccggacg gcgcgcctgg gccgcgaagt
     2041 cggtctgtcg gactgggtga agatcgaagt catcggcgac caggccacgc tgtttcccga
     2101 cgtgcaggcc accatcgaag ccacgcgcgt gctggtgaag gaaggcttta ccgtgctgcc
     2161 gtacacttcc gacgacatcg tggtggccaa gcgactgatc gatgccggag ccagcgcggt
     2221 gatgccgctg ggtgctccca ttggcagcgg acttgggatt cagaaccagc ccagcatgcg
     2281 catcttgcgc gagttgatca cggaagttcc gctgatcgtg gacgcgggcg tgggcactgc
     2341 gtcggatgcg gcgatcgcga tggagttggg catggatggc gtgctgatga atacgggcat
     2401 cgctggcgcg caggatccgg tgctgatggc cgaagccatg aaaaacgccg tgatcgcagg
     2461 acgccaggcc tatctgtcgg gccgcatgcc gaagaagttg tacgccacgg cgagctctcc
     2521 tctggaaggc gtagtgcggt aggtcgaaag acagtttcga gtttccagtt tcaagtttcg
     2581 agcgggaaca gacctggagc tgcgaagatc ggttcctacc ctattgccaa cagtgtgcga
     2641 cagaggtggg cacaccagtt ctcatcctca tactttgatt gatggaactg ggtcgcttgc
     2701 gagatcaaag caattaacca gcatgtcgac tacctcccgg ccgatttcga gcgaggccgt
     2761 cgcggcgggc gagggcacat tgcagacgtg aatcgtgcgc tggctgtgaa tgaagtggaa
     2821 gtcgtcgacc aggttgccat ttttatccac cgcctgcgct cgtaccccag agccaccggg
     2881 aataagatca gcttcggtta gctccggaac catctgctgc aaggcgcgag taaacgcgga
     2941 tttgacccaa gcacgatatt gctcttccaa tcccttgcgc cagaacttgc gtgccatgtt
     3001 ccagaagccg acatagctca gcgttgcgac agcttcggtg agatcgaccg aagtgccggt
     3061 gtagccttcg cggcgaaatg ccaaaagcgc attgggacca gcttcgacgc tgcggtccac
     3121 gtggcgggtg aagtgcactc cgaggaacgg gaagcgcgga tcggggacgg gatagattgg
     3181 atttctgatc aggtagcgcc gctccggctt cacctcgtaa tactcgccac ggaagggcac
     3241 aatctcgaga tcggtattgc agccggccag gcgagtgatc gcgtcgctgt atagcccggc
     3301 gcagttgatg acatagctgg cgcgaaacat tcctgcggtg gtttcgacaa cattggcgtc
     3361 gccgtcgtct cgtaggccaa cgaccttggc gcgaaggaca atttctccgc cagcgtgctc
     3421 gatcaactcc gcatattttt ctgccacgtc cttatagtcc acgatgccag tggagggaac
     3481 gagcagcgcg cagatgccgt cgcagtgggg ctcgatctcg cggaactcat cacggctcag
     3541 catgcgcgtg cgctcgacac cattggcgat gccacgctga tgcaattgtt cgaggcgcgg
     3601 cacttcttct ggactggttg cgaccaccaa cttgccacat tcttcatatg ggatggagta
     3661 ttcctggcag aagcgcttca tcgatgcgca gccggcgaca cagttgcgcg ccttcagcga
     3721 acctgtcttg taatagagtc ccgaatggat gacgccgcta ttgtggccag tttggtgagc
     3781 ggctacgcgg tcttctttct cgaccaccac caggcgccgg ccgggaaatc ggcgggtgta
     3841 ctcgagggcg gtggctagcc cgacgatacc tgcgccaatg atgattacgt ctgcctggcg
     3901 atcagccata gaatcctgcc tttggtgaac aggcgattat acgtagtgtg tgaatgggca
     3961 ggctgtgatg ttgaacacaa cgtttgcgtc agccaaagcg taggattcta ttcttagtac
     4021 tgattttagc ggaggatcat gcgagtactc gtcaccggcg gcgcgggttt tctcggttcg
     4081 cacttatgcg atgccatgct ggccgaggaa cactccatag tgtgtgccga caacctgctg
     4141 accggacgca tggaaaacct cgcacacctc ggtcgcgaat cgcgatttga atttctgcag
     4201 caggatgtct gcgaggcctt cgatcccggg aagatcgact atctgtttca cttcgcatcg
     4261 ccggccagcc cggtcgatta ccttaagcat ggaatcgaga ccctgcgagt ggggtcggcg
     4321 gggacgatta actgtttgga aatcgctaag aagtacaacg caaaatttct gctggcttcg
     4381 acttcggagt gttacggcga tccgctggag catccgcaga aagaaagcta ctgggggcat
     4441 gtaaactcaa tcggcccgcg ctcggtctat gacgaagcga agcgctttgc cgaggccgca
     4501 actatggcgt atcaccgtta tcacgcggtc gatacccgca tcgtgcgcat ctttaatact
     4561 tacggcccac gattgcagat cgacgatggc cgcgtgatct cgaatttcat gaggcaggcg
     4621 ttgcgcggcg aagaccttac ggtttatggc gatgggatgc agacacgcag cttttgctgc
     4681 gtaagcgatg agattgcggg aattctcgcg ttgtcaaagt cagaggagca tctcccggtg
     4741 aacattggca accccacaga atttacgatg ctcaaatgcg ctgaggtggt gctggaggta
     4801 acgggctcgg cgagcaagat tcgctttgag cttctgccgc aggacgatcc caaacagcgg
     4861 cggccggaca ttacgaaagc ccgcacgctg cttgggtggg agcctaagat cgatctgcga
     4921 accggattgc agttatcgct caattatttt cgggaggcag tgctggcaaa ctcgtcggca
     4981 gcttgcaggt agtcagcggc ggtcgggcgc agaatcccgt aaaatgcgct tgacgcagac
     5041 gaaggtaccc gcgatggacg cccaggtcga actcgacgaa ctgcaccgcg acagcgaatc
     5101 gtggtggtat accacgcggc gcaaattgct gcgcgaggcc gcactacagg ccgtccacgg
     5161 caagcgggaa gcgcgggtgc tcgatctcgg ctgtacggcg caattgcaat tcgacgattc
     5221 aacgatgtat cgggtgtgca acgtccacag ttctttgcgg acgctcgcct ttcgccagat
     5281 tgaaggtgac gataacctgg tttgttcgcg cgctgaagaa cttgcgttca cttccaactc
     5341 tttcgatgcc atcgtcgccg gtgatgtgct gcaatccctc gacgacgatg tgagcgcgct
     5401 tcgcgagatg cggcgcgtcc tcaaagaagg tggattattg tgcctgaccg cgccggcata
     5461 ctcattcttg tggggcgagg atgatgaggc ccgcgggaat catcggcgct atacggtcag
     5521 cgagttgcgt cgcaagctga cgaccagcgg gttcgaaatt catcgagcat cgtacttcgt
     5581 tgccagcgca ttcctgccgc tggcagttgg ccgggccgcc atggatatct tccgcaaatc
     5641 catcacccgc catcgtcatt atcctcatcc ctcgcaactg gcaaacgcag ctatggctgc
     5701 gctgctcgat ggcgaacgca ttctgctgca ctacatcaat tttccattcg ggacgcgcgt
     5761 ggtgtgctgg gcgctgaaac ctgcgctggt cgccgaaacg gtgaccgtcc ccgcgtggga
     5821 gcgcaagtgg gcgcagcatc cgctggcgca gggtagcggt tgagacggta aaatcaaagc
     5881 gtggccgtcc ttcgcagcat atttcgcaac accggcacaa ctctggagat gatcatgtgg
     5941 gagcactcgg tgctgacgct cccctttggc ttgacgggcg cggttctggc ggcgcgtggc
     6001 attcccaacc tgcacacttc gatctggatc atcttggcgc tggtggcggc gcggtcagcg
     6061 gcgatggcat tcaaccggtt ggtcgatgcc gacattgacg cggagaatcc acggacgaaa
     6121 atacgcgcgc ttcccgccgg caagctgtcg aaacagtttg tgctaggatt tgtggttgtc
     6181 acgtctgcga tcctgatgtt cgccgcgtgg aagttgaatc cactgtcgtt ttatctgtcg
     6241 ccggtagcgc tggcgattat ctttttctat tcgttcacca agcgattcac gcgctgggcg
     6301 catgtggtgc tgggtttggc gatgggtatc gcccccgctg cggcatggat tgcggtgcgc
     6361 ggctcgttcg atccccgcat cctgttgctg acgggcgcgg tggtgttctg gggagcgggc
     6421 ttcgacatcc tctacagttg ccaggattac gaattcgatc gcgacgcgaa catctattcc
     6481 gttccgatgg catttgggat tcggaattca ctgatgatcg cacggctgtt tcacgtcttc
     6541 accgtgctgt tgctggcagg gatggcggtg gcattcggct tgggcaagat tgcgatggtc
     6601 ggggtggtga tggttgcgtt actgctgatc tacgagcact cgctggtcaa gcacgacgac
     6661 ctcagccggg taaacgccgc gttcttcacc atgaacggct tcatctcgat ggggttcctg
     6721 ttcttcatcg ccgccgacct gcttgttgcc cggtagagga aagctttcac aaggtttcag
     6781 agccgccgat tcacgtcaca tcctcctgtg atttcgacgc cggggtgaga tatcattgaa
     6841 taaatggcaa ccgtagagca gacactcgcc gggaggaagc agcatccttt tcaaaccgat
     6901 gatccgcggc tgacgtctat cgccgaaaag gtactggcgc aggagcggct gtccgaggaa
     6961 gacgcgtttg cgctttaccg ctcgggcgac atcctggcca tcgggtggct ggcgaaccac
     7021 gtgcgcgagc gggtgcatgg cgacattgcc tacttcaatg tgaaccggca cattaattac
     7081 accaacgtgt gcgtggcggc ttgccggctg tgcgccttcg gacgcaagaa agatactccc
     7141 ggcgcctaca ccatggcgct ggaagaagtg tggaacacag cagccacggg ctataccgag
     7201 gcggtaactg agttccacat tgtcggcgga ttgcatgccg atttgccgtt ccagtattac
     7261 cccgacatga tcgcggggct gaagcagcgc tttccgcagg tgcacttgaa gggcttcact
     7321 atggtggaga tttcgttctt cgctaagatc ggcaagatga cgccgcgcga agtgctggtc
     7381 cggctgaaag aagcggggct cgactcgatg cctggaggcg gagcggagat tttctccgac
     7441 cgggtgcggc atatcatctg cgatcacaag atcgacggcg acgactggct cgacatcgct
     7501 cgcaccgcgc atgaagtagg actaaagtcg aatgcgacca tgctctacgg ccacatcgag
     7561 cacgacgaag accgcgtcga tcatctgttg aaactgcgcg ccctgcagga cgacacacat
     7621 ggattccaga cgttcattcc gctggcgttt catcccgaca acacgccgct gcagcatctc
     7681 ccgaagacta cgggtatgct cgacctgaag cagatcgcgg tgtcgcgact ggtgctcgac
     7741 aacttcccgc acatcaagtc gtattggcag atggtgacac cgaaggtcgc gcaaatctct
     7801 ttacgtttcg gcgctgacga catcgatggc acggtggtgg aggagaagat ctatcacgac
     7861 gcgggcgcga ccaccccgca ggggctgagc cggcaggacc tgatccggct gatcaccgag
     7921 gcgggacgcg tgcccttcga gcgcgatacg ctctaccgtc cagtcacgcg gaccgagacc
     7981 acggtgaccg tggcggtgta actcttttac ttctgctccg gcttcaaccg tttcccgccc
     8041 ggcatgggtg gccatcccaa cgacgctccc tggaacagag gcgtcgcctt cgcgtaacgc
     8101 ttgcccttgt cgtcgtgcac cgcgtaccgc cgctgatgat cgggcggtcc ggcccacagc
     8161 gacactcccg cataggcccc gtcattgcga aggatgtagt aggtcatgtc catgtactgc
     8221 aacttgtgca tatcgccgtt gtaattgcga acgatgcgct tgagcgcgtc gagtccggct
     8281 tcttcgggcg ccatgccgtg gcgcatgttc tcgacgatag tgtggccgcc gcagaccttg
     8341 atgttctcct cgccattgcc tgttgcacct gcagcgccta catcctgatc gcaatagcag
     8401 ccggcaccga tgatgggcga gtcgcccgcg cgcccggcaa tcttccatgc cagtccgcta
     8461 gtggtggtcg cactcgacat ctcacctttc tcattgatcg atgagcaatt aatcgttcca
     8521 gtgggaggct tcagcacgcg ctcgatggcg gccatgcgaa actcgggcgc gatgccgatg
     8581 cgggccgcga gcttctccat tttctcgatg agttgctgct gcaactcggt ggtatcgccg
     8641 ccggtatcgg gaaacttgaa gttcgggctg gctagtccgg ggccccacca gtcatcggta
     8701 gacatagtct cgcgccatag ttgccaggtc ttgcgcgagc gctcggtcag cagattctct
     8761 tttgggaagc ccatcagcaa accgaacttc gatgcgccct cgcccaccaa catgacgtgg
     8821 ccagtgtgtt ccatcacggt cttcgccagc agcgacacgt ttttgatgtc gtgcacgccg
     8881 gcgaccgatc cggccatacg cgtcggtccg tgcatgcagc aggcatcgag ttcgacgacg
     8941 cagtcttcgt taggcaggcc gccgagtccg acgctgtcgt cgttgggatc gtcctccgga
     9001 ccggtgatga cttggttgac agcatcgagc gtgtcgccac cgctgataag tacggcatag
     9061 ccgcgatcga gatagttgta accgttggcg gcgcaaataa tgaccgggga cttaggcttg
     9121 ggcccggaag cgggaggctt gtatcccggc gcctgctggg cggcatgcaa gtcgagagcg
     9181 atcgatgccg aaccgagcgc ggtggtagcg atgaatccgc gacgcgtaac cttcataggt
     9241 tctccgtaaa ggaagcgatt ggaggatagc acggcggttg agggctcggc ttatgcgcgt
     9301 cgatgagggc ggttttacct cgggaacagc ggatctgtcg ttagcaggtg cttgaagtat
     9361 tcgtctttcc aggagggatt gatgcggtcg agcagcaagg cctccgcggc gccgaaagag
     9421 taaaacacct cacgtttgga ctgcgcgata tcgatgtgct tcagttcgtc gagtgtgcgc
     9481 gatcgggcga tcaccgcata ggcgttgaat ggcgtgaagt caggcagggc ggcatacgcg
     9541 gaagagggct ggtagtcctc ggcggcctcc gcggctttca cctgggtgta acgagcgaca
     9601 ccttcctgcc aaagctgaaa gctgaagtac ttgtgatcgc cgggcaggag ctgtgcgaaa
     9661 aactgcttgc ggtccttcaa gtattgctta ctgagctgat caaacttctg cttgtcaacc
     9721 tcattcaacg catgcagaag tgagtcacga agatacccga acgactctgc gaattcggga
     9781 ttgtcatagg ggaaggggta gttgagcacc cacatgccgg tggcgtcgcc cttgctcaat
     9841 ccgagatcgg ctatggcctt caggtagtcc ggctgcgccc actgcaattg gtggaaatgc
     9901 tcgtgcatga cgacgaagag ccagggtgtg ctggttttgc attgtgtgtt ggacggctca
     9961 ccgatcacga tgacaggagg aaatccgaat gcgggaaagg tcgcctgcat gttgggaggg
    10021 aactggcgcg ggcgtgcgta aatgtcgtcg ccgattttct gaaaatcctt gggcggagtg
    10081 gggaaatgtg tcagatattc agtgtcgctt gtgacgagca ggagtagagc gggagtcttg
    10141 ctccagccga gccaaatccg gtcttggatt tgggttgcga gccgataaaa ctcggcgatg
    10201 cggaccgcgt cgtccgcttg gagcggcttc agcgcctggg cgaacattgt cgtggagagt
    10261 gcgagcagcg cgagtaggta tcgcatggga ataataaatg tgtcagggag catcgcaggc
    10321 tagccgtcac cgcccgcgga gtacagccga tgtcggccgg attccgcagg cggcgaacca
    10381 agcagcagat cacacggcga tcttggctgg cgtgttctga tcggcgcgga agcggagctg
    10441 cgccgcaatg cgaccgatct tgtcgaactg ctcatcgtca ccgatgtaga caatctccag
    10501 tccattgcgg atggcatgtc cgacaatagc gtctccgagg tcctcaagct cggtgttggg
    10561 cttgccgcac atcaggcaat cgggagccag gtgatagtcc atgtgaccgc agtgatagca
    10621 cttcacgccg ggcgcgctaa agctcgcagc caacatcagc gtctgtactt ctccggcttc
    10681 tagagaacgc aatacccggc gcagaccgat ggcgccgcgc ccgttgcggt gggcctcgcc
    10741 aatcacctct gtaatcatcg cctgcttgcg ctcttcgtca cgctgctcca gcaattcgag
    10801 cgccgcctga cgcacttgct ccggcgtggc cgacttgggg tcgatgcgga aatgccccac
    10861 cagccgctgc cttgagtagc tgtggagatg tggaaggaac tccgaccaca catcatcatg
    10921 gcagccgatc agcagcttct cgcatccgcc gtgctcaatg tgcccagaga cggcgtcaga
    10981 gacaactttg aaatgctgca tggcctcgtt catgacacgc cgctcggcgt ggccggcatc
    11041 atagcctccc cagccgtcgc tgcggccccg gcgggtcagt tcattgatga agtcgagctt
    11101 ttcgaggatg gtgccatcgg cgatctcgaa gacgcgcgcc ttggtgcgat cgaccagcac
    11161 cacgcaggca tgctgcatgc catcgaagat tgcggccagc ggcttcaggt ggaaacgctg
    11221 gttgagaatc aggttcgttt tgggcagctt ggcgggcaga tcgaactcgc gccagatgcc
    11281 cttggcggca caagcaaaaa tggccttgcc atggcggccg ttgttgcgca gcatctcggt
    11341 catctcgagg atccgctgca ggtcggcaac ggcggacttc tgtttgctcc ctttcttctc
    11401 cgccccgcgg agtgagttgt tgaccaagtc ttttacgaga atggtttcgt cacgatgcga
    11461 acggttggtg ggcgccgcgg gctgatagta aaacgttacc gcgcagccct ccggcgaaag
    11521 gaaattagcg agctcacgga tgtcatctct cgtgatcatt ccggttctcc tgggtgaatg
    11581 ttggcttcat ggttcggagg gaccgccaac aagcggcccc ttcaggtggt atgggtatac
    11641 tccacgtgct tggttttgaa aaggtcctgg atgggcaagg cgcgttgcag atgctggcgg
    11701 gccttgcgga tggacgcgtg cacctcctcc agcgaatggt tggtaatggt ggcaatctct
    11761 tcctcggcaa aaccctcgac cgcataaagg atgaagacct cgcggtcgga acgcttggca
    11821 tcgcgcaaag caagttgcac cacggcgatc aactccccgc gggcagtcat ctcctcggga
    11881 ttgccggcat ggccgtcagg aatggtgtct tcttccagca gccgttcgtc aggctggtgg
    11941 aactgcagcc ggacttcgtc gctagccaga acattttgcg tgccgtggcc gcgttcgagt
    12001 ggaaccatgc cgccgccgtc atcgctggcg gacatgcggt cgatggcctc gctactcaag
    12061 cgataaagcc agggctcgat cttgatgcgc tcgggctttt ccatttcatc gcccagtgcg
    12121 gtggcgattg cctcggccac cacatcctct ggtgctacct ggtcgggcag cagctctccc
    12181 aggctttcgc gatagttgat ctggcgttcg acgaagcgtt gcaggcgcgg caagttgatg
    12241 tcgatgtagc tggcgatgtc gagtgacgag acggtctcgg gacgaacgac cgcgagcgtg
    12301 tcttcgaatg gcacgttacc ggtgccggtg cgtcctgcgt cacggcgacg cacccacttg
    12361 tgtttgctgc gcagcacctg cttgtgtcgc ttgagctgct cgatcagcgc atcgaatgcg
    12421 gccctgattg ccgcagccgc ggtcgcgctc ttctcctgcg cggccatctg cccggaaggc
    12481 aagcgcaggt tgagcgacac gacgaagccc tcccgcgccg agttttcttc gacgatgcct
    12541 ttgacatgca ccagttcggg acgaaatacc tgcaggtgct tgctcagttt ttccagttga
    12601 tgttgggttt gtttctcgag gtcggcagtt ttggaaatct tgtaactaaa atgaacgttc
    12661 atgaaaggtt ctccggaggg aaagttccaa ccgtagctac cttgctctga ggccagttta
    12721 ctcctgttaa gaagctgtca actgagaaat gaacggtggg acgcttttta gggaccggag
    12781 tgggagggaa aaatgccgtt cccgttactg actcgggatc agcctttcgg cgggagatga
    12841 tattacctta cggccgagcg ttgggccgga agttgcaaag ctcagcccgc gaaaaaggcc
    12901 ctccggcgga ggagggcctc tggtttagcc tgattcgggt cgcggttacg ggatgcgcac
    12961 tttcgcgtcc gggctcgggt tgacgtgaat gctttcgata aatcgtacct tacggttctg
    13021 gagcttcatc accagggagg aggtacgaac gccatcgccg aagaagcgca cgcctttgag
    13081 cagagggcca tcggtaacac cggtagcagc gaagacgatg ttcttgcccg gcgccaactc
    13141 gtcggcggtg tagatgcgct tggggtcctt gatgcccatg tctgacatgc ggtccgcctg
    13201 ctccggagtg tagccggtca ggcgacctac catgtagcca ttgaggcagc gaatagcggc
    13261 cgccgtgatg acgccttcgg gagccccacc gattcccatc acggcgtgga cgcccgagcc
    13321 caacatgccg gcggcgattc caggtgacaa gtcaccatcc gagatcagtc gaatgcgagc
    13381 ccccgccttg cgtatatctt cgatcaactt ctcgtggcgt ggccgttcca ggatgatgat
    13441 gaccaagtct tcgactttgc gaccaagcac cttcgcaatc accttaagat tgtgcccgac
    13501 cggcgcctcg agatcgacca cacccttgca cgagggaccg acgacgatct tctccatgta
    13561 gcagtcaggc gcgtgcagca ggccaccgtt ctcggaagcc gccagaaccg tgattgcgcc
    13621 gggagacccg gtggcgcaca ggttggtgcc ttcgagtggg tcgaccgcga tatccacctg
    13681 gggatactgg ccgccgtcat gcttgccgac cttctccccg atgtagagca tcggagcttg
    13741 gtcacgctcg ccctcgccga tgacgatggt gccgtccatg ggaacggaat ccatgacacc
    13801 acgcatggat tcggtggcca tctggtcggc caattcgcgc ttgccctggc ccatggtctt
    13861 ggccgaggcg atagcagatt cctccacaac gcggagaaat tccagcgcaa ggtcggcctc
    13921 aaaatttgtt ctcggagtgt agtgattgat atcgattgct ggtttcggtt cagcgtggac
    13981 cttggtagcc atgtggtctc cgtagagttc gcggcttgcc ggggcgggtc agcggtcgca
    14041 ggggattagg ttaagagagc ttgaggggcg gttaggctcc tcgatccagc ccaaacttta
    14101 aggatacaca aatatgttcc cagcggcgtg gacgaaaatt tcgcgacaac ttgctggcta
    14161 tacgagttgg caagaagacg gagtctagtt cagaatgtgg tctctgcccg cgaaatttcg
    14221 cgtgacaaag gtctacactg atcgagcctc agcggggtga ccggatttgt ggtccggaac
    14281 tgctgggacc tgggcacccg gcgccgcgtg gttacaactt gcgaagaggg ctttcccaag
    14341 ccttttgtga atacaaccgg tcttccaatc attgaacgga tgccagggtg gaagggacgt
    14401 tatttccatt cggccaacat gacattcgcc cattacgagt tctcacgtgg atcttctatt
    14461 cacgagcact ttcatcctga agaagaggtg tacgaggtga ttgatggcga acttgagata
    14521 acgatcgatg gcgttagtca gaccgcaagg gctggtctgg tggccatcgt gccgagcaat
    14581 ccccgtcatt ctgtgaaggc gcttacggac gggcgggcaa tcattgttga ccatcccgcg
    14641 cggcccgaat tcggctaata ctgatctcga cgtaaggcgc tggcagcgcg gaaacctgta
    14701 agcggaggac tcatgacccc agctatccaa caatcgatgg agttcgacgt ctcgcctgag
    14761 acgctgtatg agctctacat cgattcgaag cagcattcgt tagcgaccgg tgctcccgcg
    14821 aaattgggcc gcagaacagg tgacgccttt accgcgttcg gcgggcagct taagggccgg
    14881 aacttgctgg tggttccgaa gcagatggtc gtgcaaacgt ggcgtgcgga aggatggaag
    14941 aaggccgacc cggattcgat cctggtgatc cgcttcagca agacgaagaa cggagcgcgc
    15001 gtcgatctgg tacacgtcga tgtccccgaa tacgaccatc aaggcgtcac cgacggctgg
    15061 cgtcattact actgggagcc ctggcgggtg tatctggaag cggttgggct gaagcagaaa
    15121 gctggcgccc gctccaaacg gaagggatag cttcgtggtt atctagaacg ccgcaatccc
    15181 cgtgatatgt tcgccggcta ttaacgtgcg gatgtcgtgg gtgccttcgt ttttttgctg
    15241 actccaagcg tgccctgggc gtacagtgag aaaaactaaa gcgaggtaca ccgcatgcga
    15301 gaattgctcc gcttctgggc gaaacatttg ctggttgtcg ttctagcttg cgggatcatt
    15361 ccgttggttg catctgcggc cgacgaaact tcgctgacca aagatcagat cacggagttt
    15421 ctccagaccg ccgaagtcat caagagcaag ccctcgagca agggcgtcac tcatccctca
    15481 cggctcaccc taagcaacgg caccatcact cacgacgctt cgttccagac catcgacgag
    15541 cactcggagc tgaagcagct cgaaagcgga catagggaat acaatttcgt cgactcttac
    15601 aaattcaaca tcgcggccta ccggctgtct gaacttctcg gcatcgacaa catgcttccg
    15661 gtctacgtgg agcgcaaatg gcaagggaaa accggttcgc tcagttggtg gctgccggtc
    15721 cagatggacg aagccgatcg catggcaaag aagattgtgc ctcccgatat ccatgcttgg
    15781 aacgagcaga tgaatcgcat ccgcgtgttc gacgaactta tctacgacac ggatccaaac
    15841 ctcaccaacg tgttaatcgg agaagactgg actgtctggc gcatcgattt cacccgcgcc
    15901 ttccgcacct acaaggacct gcgcctcccc caagatctcg tgaagtgcga ccgccagctt
    15961 ttcgaaaagt tgaaggccct gaaaggcaat gaactggcgg acaaaaccaa gaactatctg
    16021 accaaggatg aagtgaacgc cgtcatggcc cgccgcgaca agatcgtcgc catcttccag
    16081 ggtcttatcg ccgaaaaagg cgaaaaagaa gtcctctact agtcctgccc gagcgcgtaa
    16141 acgcgacctc tcgtcagggt ggcgcttggc tggctcatgc gtacccggag agtgtttttt
    16201 tatcttgtcg aaattccttg gatttatcaa gttagcggga atttctcggt aaaatccggc
    16261 gctacaataa cttacgggca aaatcttcat ttcaaataac ttagtgggtt ggctcctcgc
    16321 ggccagcccg cgcccgatac atcggacgac agccatccag cattagtgat agagcagcca
    16381 ttcacaagag tcacgaagaa tcgtgggggt gaaaaaacta aagtaacttc gcgtttccga
    16441 aacgggcgaa gcggcagggg gccctgcctt tcggcctgag atgatacatc gtctctttcg
    16501 ctggggtgcc ccacccttgt cgccggcccg tctctccgtg ctccacacat tgaagtcgta
    16561 gtagcgaggt tgctagaaag gagtatcggg cgtgtgagca actatatgcg ccacgttctg
    16621 cttaagcgtc tgcaaagcta ccgccagagt actgcacctg ggctcactca gcagtaggtg
    16681 aacgtgttcc ggcatgacga cataaccatt gacgtaaagg gccgcaccag cgcgcacctg
    16741 ttcgagaatt tgttcaaacg tgcggcgtgc ctgctgggtc gccaatagat gtgctcgacg
    16801 gcaacaaact tgcgcgcgaa atcgtccaaa ccgccgatcc taagggtttt caagggggca
    16861 cgtaacccaa ctttcagaac taacggcgat gattatcgaa tagtaaacat catttcgctc
    16921 atagagagtc gccttagcta actgccgatt atcaattact aattactggt tcctcgtcac
    16981 catccgaaag aacctctgcg ctcgcgcaac gtctcgtccg atctgctcgc gcagcgcttc
    17041 caccgattca aacctgatct cctcacgcag ccg
//
LOCUS       DASRKK010000823         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_191387, whole genome shotgun sequence.
ACCESSION   DASRKK010000823 DASRKK010000000
VERSION     DASRKK010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191387"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFC15_19715"
     CDS             complement(<1..969)
                     /locus_tag="VFC15_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12441.1"
                     /translation="MPYRYVRVFFAVFLGFTLTLFAVAADKSKSAAANANQPSYDLPQ
                     PQRETLDYGMYQRIRDEGLGHSHVMEYASALDDDIGPRLTGSANLKHANEWTRDQFTA
                     MGCSNAHLEDWGEFGMGWQQLNTWIRMTAPDTAVFIAQAAPWSPATNGAINAPAVWID
                     VKDAKDLDQYKGKLAGKIVLYGEMRDVPPVDKPLFVRLDDAELKKLTEYPLKPRGRDF
                     IEQYMKRLELRQKVGQFLADEHALAVVTPSRDGRDGGGSGGTVFDDSGAGFGWEAYKR
                     ERANPLPVVVMAIENYGRVYRLLKARVPVTIEMNIDTKFTGDHEHGY"
     gene            1400..1846
                     /locus_tag="VFC15_19720"
     CDS             1400..1846
                     /locus_tag="VFC15_19720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM12442.1"
                     /translation="MDQSDARAMITSIDTNVIIALWDRDLAISSAAQSALDNALERGG
                     LVVGAPVFAELMAAPGRGETFLTSFFRDTGIAIEWNLQEAVWRAAGRSFQKYAARRRG
                     KREPGPRRILADFLIGAHASERGYRLLTLDDRLYRAAFPGLALVRV"
     gene            complement(2311..>2649)
                     /locus_tag="VFC15_19725"
     CDS             complement(2311..>2649)
                     /locus_tag="VFC15_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12443.1"
                     /translation="FEIKVPASIANLGPGFDTLAVAVQLYLRLCVRTVPGRGKLEFRF
                     VGQHLCGENYIERAFRFLARQDSNSFIAFASAISSSETSMTNPLLCWTARSAFFHERG
                     LPIRIAVAAA"
BASE COUNT          563 a          762 c          781 g          543 t
ORIGIN      
        1 gtacccgtgc tcgtggtcgc cggtaaactt agtgtcaatg ttcatttcaa tcgtgacggg
       61 gacgcgcgcc ttcagcagcc ggtacacgcg tccataattc tcaatggcca tgaccacgac
      121 cggaagtggg ttcgcgcgct cccgtttgta cgcctcccag ccaaaaccgg cgccactgtc
      181 gtcgaaaacg gtgccgccag agccgccgcc atcacggccg tcgcggcttg gggtgacgac
      241 ggccagggcg tgttcgtccg ccagaaactg tcccaccttc tggcgtaact ccagccgctt
      301 catgtactgc tcgatgaagt ctcggccgcg cggcttgagc ggatattcgg taagcttttt
      361 cagctccgcg tcatctagtc gcacgaagag tggtttgtcg accggaggca catcccgcat
      421 ctcgccatac agcacgatct tcccggccag tttgcccttg tactggtcga ggtctttcgc
      481 gtcttttaca tcgatccaga ccgctggtgc attgatcgcg ccattcgtcg ccggagacca
      541 cggggccgct tgggcgatga acaccgcggt gtcgggcgcc gtcatgcgaa tccaggtgtt
      601 cagttgctgc caccccatgc cgaactcgcc ccagtcctcg aggtgcgcat tgctgcatcc
      661 catcgcggtg aactgatcgc gcgtccactc attggcgtgt ttcaagttgg ccgagccggt
      721 caaacgcggt ccgatgtcgt cgtccagcgc cgaggcatac tccatcacat gagagtggcc
      781 gaggccttca tcccgaattc gctggtacat gccgtaatcc agcgtctccc gctgcggttg
      841 aggcaggtca taggatggct gattcgcgtt cgctgcggcg gatttggatt tgtcggcagc
      901 aacggcgaag agggtgaggg tgaaaccgag gaagacagca aagaaaacgc gcacgtatcg
      961 ataaggcatc gggcgaagaa ctccattcca agcgaagatt tccaatgttg cagagtacca
     1021 gatgagaaaa cgccgcacca ttcgcgctca ggtgcgatcc ttcaaattcg gaatcggcgc
     1081 ctatccctaa ggtagaagaa gcttgggcga agcgcccagc tatcctaacc ccagttcaat
     1141 tcaaggtaag gagcattgtg ataaagtaag gagagccaga gggaagattg caaaagcagg
     1201 caaaaatcac gagcaagggt cagattaccg tgccccacga gatccgccgc gtgctgggtg
     1261 tgcgaacggg cgacgggttg atctttgaaa gcgacgcagc gggggtccga gtccggcccg
     1321 tgagggcgga aagcccattt gcaaaatatc ggggaatcgg caatccggga atcccgtcgg
     1381 gtcgggccgg aatcaatcgt tggaccaaag cgacgcgcgg gcgatgataa cttccatcga
     1441 taccaatgtc atcattgctc tgtgggaccg cgatctcgct atcagttcgg cggcacaatc
     1501 cgctttggac aacgcccttg aacgcggtgg cctggtggtt ggcgcgccgg tcttcgctga
     1561 actaatggcc gcgccgggcc gcggcgagac cttcctcacc tcattcttca gggataccgg
     1621 tatcgccatc gagtggaatt tgcaggaagc agtttggcgc gccgcaggac ggtcttttca
     1681 aaaatatgcc gcccggcgaa gaggaaaacg ggaacccggc ccgcgccgta ttttggctga
     1741 cttcctgatt ggcgctcacg cttccgagcg cgggtatcgg ctgctcaccc tcgacgatcg
     1801 tctctatcgg gctgcctttc ctggtctcgc acttgtacgg gtgtagcctg ggctcagcca
     1861 gcgtccaacc tttcgaaatc ttaccgaaat tagggaaggt cgggtgaggc atcgggcgaa
     1921 cgactccatt ccaagaagaa attaatttcg gaacggtaac ggatagatcg ctctcgaaaa
     1981 gggcggcaac gccaagctgc agatgatggc accttcgatt tgcaacggag gtggttgggc
     2041 tgctgcatta ggaagcgaat tcccacaatg gaacgccgat tttactttag agcgcgccat
     2101 ggcagccaga tgtagttttt catcttcctc aagccaaatc gtcgcgctag gggctccaga
     2161 acgcccgcaa atggcaccct acgcgatgct gtagctaaca ctttagcatt gggcatgcat
     2221 ggcactaatt ggcccatggc ggttgggcct tcttcccagc acgggattct ttcgacgact
     2281 ccgcttgata cgggcatgat ttagtttgac tcaggcggct gcgacggcga ttcgtatcgg
     2341 caatccgcgc tcgtggaaga aggcgctgcg cgcggtccag cacagcaacg ggttcgtcat
     2401 ggacgtttcg gacgaagaga ttgccgaagc gaaagcgatg aaggaattgc tgtcctggcg
     2461 cgccaggaac ctgaaggccc gctcgatgta gttctcgccg caaaggtgct ggcccacaaa
     2521 gcgaaactcc aacttgccgc gtcccggcac cgttcttacg caaaggcgca ggtacaactg
     2581 caccgcaacg gcgagcgtgt caaaacctgg ccccaagttg gcaatcgatg ccggaacctt
     2641 aatctcgaa
//
LOCUS       DASRKK010000824         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_680554, whole genome shotgun sequence.
ACCESSION   DASRKK010000824 DASRKK010000000
VERSION     DASRKK010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680554"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            673..1023
                     /locus_tag="VFC15_19730"
     CDS             673..1023
                     /locus_tag="VFC15_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12444.1"
                     /translation="MDEQAQVVSVRRRRSRGEAEQLVAEYEASGLSRVDFCHERGLSL
                     ATLARYRKRHSQANAAPGVRWFEVEVSGNGPAPESGGVSGLTVALKHGRRIEIARRFD
                     ARTLAQLLAVLERV"
     gene            1026..1400
                     /gene="tnpB"
                     /locus_tag="VFC15_19735"
     CDS             1026..1400
                     /gene="tnpB"
                     /locus_tag="VFC15_19735"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZM12445.1"
                     /translation="MFGWGPATRIYLAAGATDMRKGFEGLYGLVRDRLQCDPLSGHVF
                     LFANAQRNRLKLLFWDGSGLWVCAKRLEKGRFRWPRAADAQTKVALSHEELALLLGGI
                     DLADTTRRPWYRKAGPDIQISA"
     gene            1462..>2903
                     /locus_tag="VFC15_19740"
     CDS             1462..>2903
                     /locus_tag="VFC15_19740"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM12446.1"
                     /translation="MSGLSSTPTSESERNIQLQNELAWAKMKILSLEAQLRLQRIKKY
                     GPGSEKLDSAQLELLELEPGVSRLEVQAESQRDPLPAARAKSHKHPGRQELPAELPRV
                     ERVIACTPEQRTCKACGQETAVIGYETSEQLDVEPAKYFVVVTKREKRACKCCQEGGV
                     TAAAVPARIVEKGLASDRVVIDTVVAKYSDHLPLYRQSAILDREAGVEISRATLDGWV
                     MRVGELLIPIVSVMRRELLGGSYLQADETTVDVQMHDGSGHNHQAYLWQYGRPGSGVV
                     FDFRLGRGREGPKKFLGQFEGILQTDGYSAYDGVGGPKMVHAACWAHARRKFFEAAKL
                     DPGDLVAVRMVAWIDELFGIDAEARQRKLDHAGRHALRLERATPLLATISEEIETVRT
                     TALPSSALGRAAKYTFSLWRKLTRFLDYPELELSNNLAENSMRPVALGRKNWIHVGSK
                     DAGPKVAAIASIVETCRRLKIPIREYLGGV"
BASE COUNT          583 a          818 c          975 g          527 t
ORIGIN      
        1 gtcctaaggg caagcggaat acggacattg gccttgccac cgcggagtcc gaatctgaac
       61 gctttctccg agcgctgggt gtgttccctc cgtcaagaat gcctgtccaa actcattctg
      121 ttcggagaaa cttcgttacg ccgggcgttg aacgaataca tcgaacacca tcattctgaa
      181 cggaaccacc aggggaaggg caaccttctc cgattacctt ctccagacct accgcgaagg
      241 tccaagagcc gtccagcttg ggatgttggg cagtcttatg ttttcagtag tgtataactg
      301 cgcatggcgt tgattcaggg catgaaaaac gccaacaaaa cacgatctcg tttcgaacgg
      361 tttgctaatg gagggttttt ggagggttag cgccaaccct ctgggagtcc gacggcatcc
      421 gcgccgaatc gccacgcggt ccgtccgcgc gtcgagaaac attctttgtg gcccaccgct
      481 tggacacgcc ctgattgcag aactgggcta agcgtcgcat cacagctctg atgtccaagt
      541 gcccggccaa tgcctggacc tgcgattccg ttctcggatt gcgcgaatcg atcgcgcact
      601 cctggcgatc ctgcgtatgc gtcaagccaa caccatctgt acagcggcgg gcgttggtga
      661 tgcagtgatg gggtggacga acaagcgcag gtggtatcgg tccggcggcg ccggagtcgc
      721 ggggaagctg agcaactggt ggccgaatac gaagccagcg gcctgagccg cgttgacttc
      781 tgccacgagc gaggcctgag cctagccacg ctggctcgct atcgcaaacg gcattcgcaa
      841 gccaatgccg cgcccggagt gcgctggttt gaggtcgagg tatccggcaa tggtccggcg
      901 ccggagagcg gaggagtcag cggcctgacg gtggccctga aacacggccg tcgaatcgaa
      961 atcgcgcgcc ggtttgacgc ccgcacgttg gcgcagttgc tggccgtgct agagcgggtc
     1021 tgagtatgtt cggttgggga ccggcaacgc ggatctatct ggcagcgggg gccacggaca
     1081 tgcgcaaagg cttcgagggc ctctacggtc tggtgcgcga ccggctgcaa tgcgatccgc
     1141 tgagcgggca tgtcttcctg tttgccaacg cgcaacgcaa tcgtctgaaa ctgctgttct
     1201 gggatggcag cggcctgtgg gtatgcgcca agcgcctgga gaagggacgg tttcgatggc
     1261 cgcgggccgc cgatgcgcag acgaaggtgg cgctcagcca cgaagagctg gcgctgctat
     1321 tgggcggcat cgatctggcg gacactacgc gccgcccatg gtaccggaag gccggtccag
     1381 acatacagat atccgcataa gttatccacg catattagtt gtaagtagtc gtcatgcgag
     1441 ttatactaag acttacctcg catgagcggc ctctcttcca ctccgacatc cgaatccgag
     1501 cggaacattc aactccagaa tgagcttgcc tgggccaaga tgaagatcct ctctctggaa
     1561 gcgcagttgc ggctgcagcg gatcaagaag tacggccccg gcagcgagaa gctcgactcc
     1621 gcgcaattgg agcttctgga actggagccc ggggtgagcc gcctggaggt tcaagcggag
     1681 agccagcgcg atccgctgcc ggcggcaagg gcgaagagcc acaaacatcc aggacggcag
     1741 gaactgccgg ccgaactgcc gcgtgtggaa cgcgtgatcg cctgcacacc ggaacagcgc
     1801 acctgcaagg cctgcggcca ggagacggcg gtgatcggct atgagaccag cgagcagttg
     1861 gacgtcgagc ccgccaaata ttttgtggtg gtgacgaagc gcgaaaagcg cgcctgcaaa
     1921 tgctgccagg aaggcggcgt gactgcggcc gcagtgccgg cccggatcgt cgagaagggc
     1981 ctggcgagcg accgggtggt gatcgacacg gtggtggcga agtacagcga ccatctgccg
     2041 ctgtaccgcc agagcgcgat cctggatcga gaggcgggcg tggagatcag ccgggcgacg
     2101 ctggatggat gggtgatgcg ggtgggcgag ttacttatcc ctatcgtctc cgtgatgcgc
     2161 cgcgagttac tcggaggaag ttatcttcaa gcggatgaga caaccgtgga tgtgcaaatg
     2221 cacgacggca gcggccacaa ccatcaggcg tatctgtggc aatacggcag accgggaagc
     2281 ggcgtggtgt tcgacttccg cttggggcgt ggacgcgaag ggcccaagaa attcctggga
     2341 cagttcgagg gcattttgca gaccgacggc tatagcgcct acgatggcgt gggcgggccg
     2401 aagatggtgc atgccgcctg ctgggctcac gcgaggagga agttcttcga ggcggccaaa
     2461 ctcgaccccg gcgatctcgt cgctgtgcgc atggtggcgt ggatcgatga gttgttcggc
     2521 atcgacgccg aggcgcgcca acggaagctc gatcacgccg ggcggcatgc actgcggctg
     2581 gagagagcca cgccattgct ggcgaccatc agtgaggaga tcgagacggt ccgcaccact
     2641 gcgctgcctt cgagtgcgtt gggtagagcg gcgaagtaca cgttctcgtt gtggcggaag
     2701 ctcacgcgct tcctggacta ccccgagttg gaattgagca acaatctggc ggagaattcc
     2761 atgcgaccgg tggcgctggg ccgcaagaac tggatccatg tcgggagtaa ggacgccggg
     2821 ccgaaagtgg cggccatcgc gtccatcgtg gagacctgcc ggcggctgaa gatcccgatc
     2881 cgcgagtacc tgggcggggt ctt
//
LOCUS       DASRKK010000825         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_680953, whole genome shotgun sequence.
ACCESSION   DASRKK010000825 DASRKK010000000
VERSION     DASRKK010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680953"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFC15_19745"
     CDS             <1..925
                     /locus_tag="VFC15_19745"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZM12447.1"
                     /translation="VIITDSQHGRAMPQVEEINDRLQGHVKPLLEQSRVPIMGGFIGS
                     TREGIPTTIGRGGSDFSAALVGAGLDADRIEIWTDVDGMKTTDPRICPEARRIKVISF
                     EEAAELAYFGAKVLHPATVLPAVEKNIPVLVLNSRNPSNEGTRILSRTPRSHSLFKAI
                     AAKNRITIVEIVAARMLMAHGFLAKVFEIFARHRCAVDVVSTSEVSISLTVDSNEAIP
                     QIAADLEKLAFVKYSGRKAIVCMVGENLRDTPGVAAQVFDAIRDINVRMISQGASEIN
                     LTFVVDEADVPEAVRRLHAKFFAEVDPEVFD"
     gene            922..1617
                     /locus_tag="VFC15_19750"
     CDS             922..1617
                     /locus_tag="VFC15_19750"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZM12448.1"
                     /translation="MSNRTLNLLVLGSGKTGSVVAEVARERGHQVQSIDADVNQDAAW
                     LTPEHLRDVDVAIDFTAPDAVLANIEACVRARKAIVVGTTGWYGELDRVRQAVEQAGT
                     GFVFGANFSYGVNLFFQIAQAAAPALAHNYRGHIAERHHVHKKDAPSGTAVALQRLLE
                     QASGARVEITSEREGDVTGTHTLELTSSGDHIVLTHEAKSRRTFAEGAVLAAEWIAGK
                     QGSYDFKDIFTQL"
     gene            1815..2720
                     /gene="dapA"
                     /locus_tag="VFC15_19755"
     CDS             1815..2720
                     /gene="dapA"
                     /locus_tag="VFC15_19755"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZM12449.1"
                     /translation="MKLRGCGTALVTPFKQDGSVDEAALRELVRWQVESGIDFLVPCG
                     TTGETPTLSHDEWLRVVDLTIETVAGRVPIMAGATSNNTREAVAKAKEISARPGVGYV
                     LTATPYYNKPTQEGVFQHFKAIAEASAKPLVLYNIAGRTGTNIETSTLLRLAEVPNIA
                     GVKEASGNISQIAEACATVPEDFLVFSGDDAVTLAVIGLGGVGIISVVSNEIPAEMAR
                     LTQAALANKWDTARLLFRKYLPLMQANFIESNPGPAKAVLAMMGRIEEVYRLPLVPMK
                     KENRARLEKVAAEVGLLQQHPAATH"
     gene            complement(2978..3370)
                     /locus_tag="VFC15_19760"
     CDS             complement(2978..3370)
                     /locus_tag="VFC15_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12450.1"
                     /translation="MTYSSALLRRLIKPAVLAVVLFFICGIAGWAQTTGESAPQLPGV
                     SRHRGVSQPPDEDAATAQQTRDMAKKQNLKRQQDLKKDTDKLLELATELKQYVDKTNE
                     NMLSLEVIKKAEAIEKLAHSVKEKMKGQ"
     gene            3548..>4143
                     /locus_tag="VFC15_19765"
     CDS             3548..>4143
                     /locus_tag="VFC15_19765"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZM12451.1"
                     /translation="MLSGWGDDTFDFVRHQVPRLIAVLIIAFILTRLLKIASRHLAEL
                     SNRQGLPSALRAQQLRTLSSVIYSVGVFVIMFLAALQILPLLGINMGPLLASAGIAGL
                     AIGFGAQTLVRDFINGFFILVENQYDIGDTIKLASVQGVVEDMTLRRTVLRDDNGSVH
                     TVPNGEIRIVTNLTRDWTQLPMHISVAYGADSDEVIKLL"
BASE COUNT          817 a         1228 c         1220 g          878 t
ORIGIN      
        1 ggtaatcatc accgactcgc agcacggccg cgccatgcct caggttgagg agatcaatga
       61 tcgcctgcaa gggcacgtta agccgctgct ggagcagagt cgcgtgccga tcatgggcgg
      121 cttcattggt tcgacgcggg aaggtattcc aacaacgatc ggccgtggcg gctccgattt
      181 ttctgcagcc ttggtcggcg ccgggttgga cgcagatcgc atcgagattt ggaccgacgt
      241 ggatggaatg aaaaccaccg atccgcgtat ctgtcccgag gcgcgccgca tcaaagtcat
      301 cagcttcgaa gaagccgccg aactggccta cttcggcgcc aaggtgttgc atcctgcgac
      361 cgtgcttccc gcggtcgaga agaacatccc cgtgctggtg ctgaactcgc gcaacccgtc
      421 gaacgagggt acgcgcatcc tgtcgagaac gccgcgctcg cattcgctgt tcaaggcgat
      481 cgccgccaag aaccgcatta ccattgtcga aatcgttgcc gcgcgcatgc tcatggcgca
      541 cggctttctc gccaaggttt tcgagatctt cgcgcgccac cgctgcgcgg ttgacgtggt
      601 atcgacctcg gaagttagca tctctctcac cgtcgattcc aatgaagcca tcccccagat
      661 cgctgccgac ctcgagaaac tcgcctttgt gaagtactcg ggacgtaaag cgatcgtgtg
      721 catggtgggc gagaatctgc gcgacacgcc cggagtggca gcgcaggtat tcgacgccat
      781 tcgcgacatc aacgtgcgca tgatttcgca gggcgcgtcg gagatcaacc tgacgttcgt
      841 cgtggatgaa gccgacgtgc cggaagcggt acggcgattg cacgccaagt ttttcgccga
      901 ggtcgatccc gaggtgttcg attgagcaac cgcacgctga acctgcttgt gctcgggagc
      961 ggcaagaccg ggtccgtggt ggccgaagtg gcgcgcgagc gtggacacca ggtgcaaagc
     1021 atcgatgccg atgtcaacca ggacgcagcg tggctcacac ccgagcactt gcgcgacgtt
     1081 gatgtggcaa tcgatttcac tgcgcctgat gcggtgttgg cgaatatcga agcctgcgtg
     1141 cgcgcgcgca aggcaatcgt tgtcggaact acgggctggt acggcgaact tgatcgcgtc
     1201 cgccaagcag tcgagcaagc cggcactgga tttgtcttcg gggcaaactt ctcgtacggc
     1261 gtgaacctgt tttttcagat cgcacaagct gcggcgccgg cgttggcgca caactaccga
     1321 ggacacattg ccgagcggca tcacgtccac aagaaggatg cgccttccgg caccgccgta
     1381 gctctgcaac ggctactaga gcaggcatcg ggagcgcgcg tcgagattac ttccgagcgc
     1441 gaaggcgacg ttaccggcac tcacacgctg gagttgactt cgtccggcga tcatattgtg
     1501 ctcacgcacg aagccaagtc gcgccgcact tttgccgagg gcgcggtgct cgcggccgag
     1561 tggatcgctg gcaagcaagg ctcctacgat ttcaaagaca tttttacgca gctctgactg
     1621 cttttctcac cacagagaca cggagaagaa tgttctcatc acggagaaca cgaggcgcac
     1681 ggagcagaaa taaccaatag aaactccgtg atctccgtgt cctctgtggt ggaaaaaaga
     1741 attttgttct tctctgcgtc tctgtggtga tcctggtttt tgagattcac cgctccgggg
     1801 attatcctgc aggaatgaaa ttgcgtggat gcggtaccgc tcttgtcact cccttcaagc
     1861 aggatggaag cgtcgatgaa gctgcgctac gcgaactggt gcgctggcag gtggaatcgg
     1921 gcatcgactt tctggttccc tgtggcacga ccggcgaaac gccgactttg tcccacgacg
     1981 aatggctgcg cgtcgttgac ttgaccatcg agacggtggc gggccgcgtg cccatcatgg
     2041 cgggcgccac ctcgaacaac acccgcgaag ctgtagccaa ggcaaaggaa atctcggcgc
     2101 gtccgggtgt cggttatgtc ctcaccgcga cgccgtacta caacaagccc acgcaggaag
     2161 gcgtgttcca gcacttcaag gccattgccg aagcctcggc gaagcctttg gtgctgtaca
     2221 acatcgcggg gcgaaccgga accaacatcg agaccagtac gctcctccgg ctggccgagg
     2281 tccccaacat cgccggggtg aaagaggcga gcggaaacat ctcgcagatt gccgaggcct
     2341 gcgccacggt gccggaagac ttcctggtct tctccggcga tgacgccgtc accctggcgg
     2401 tgattggact cggaggggtc ggcattattt cggtggtttc gaatgaaatt ccggcggaga
     2461 tggcgcgcct gacgcaggcc gcccttgcca acaagtggga caccgcccgc ctgctcttcc
     2521 gcaaatatct cccgctgatg caggcgaact tcatcgagtc gaatcccggt ccggccaaag
     2581 ccgtgttggc tatgatgggc cgcatcgaag aggtctatcg ccttccgctg gtgccgatga
     2641 agaaagaaaa tcgcgcccga ctggagaagg tggcggcgga ggttggcctg ctacagcagc
     2701 acccggcagc cacgcattga tagctccccc atgtcggttg agtgagcctc ggtagggtgt
     2761 cggactttgt cccgtccctg aagggactcg gatctatttt ccgctttacc cagcactccg
     2821 cctgcggctc cgtgctgggc tagactatgc cgcgcctacg gcgctgaatt tgccgttaac
     2881 aagttccagt ggcaactact aagtcttgtc ctcacagacg ctctttaggc cggctgtagg
     2941 cgtacatgcc tgctacgtca atcctcgaag cacttcttca ttgtcccttc atcttctctt
     3001 tgacgctgtg agccagtttt tcaattgcct cggctttctt gatgacttcc agcgacaaca
     3061 tgttctcgtt tgttttgtcc acgtattgct tcagttcagt tgccagttcc agtagcttgt
     3121 cggtgtcctt cttgagatct tgctggcgtt tcaggttttg cttttttgcc atgtcgcgcg
     3181 tttgttgggc cgtcgccgcg tcctcatcgg ggggctgaga cacgccccga tgccgagaca
     3241 cacccggaag ctgaggagcg gactcgccgg tggtttgtgc ccagcccgcg attccacaaa
     3301 tgaagaagag cactactgca aggacggcag gctttatcaa cctgcgcaac aaggcggatg
     3361 agtatgtcat aagaatcgag attatattcc gcgcgctggc tccgatggga ggagcacctt
     3421 tgccacttgt gctgtcatcc gctactctaa actcgattac gaatcgagga aaaggctgcc
     3481 cacagtgctg caacctctgc aagccatgtt gatgtttccc caccacacta aagaagggac
     3541 cttccggatg ctcagcggct ggggcgacga cacgttcgac ttcgtgcgcc accaggtacc
     3601 gcgcctcatc gccgttttga tcattgcctt cattctgacc cggctgctca agatcgcgtc
     3661 gcgacatctg gccgagctca gcaaccgtca gggactgccg tccgcgctca gggcacagca
     3721 gcttcgcacg ctgtcgagcg tcatctacag cgtcggggta ttcgtcatca tgttcctggc
     3781 agcgctgcaa atcctaccgc tgctgggcat caacatgggg cctctgctgg ccagtgccgg
     3841 catcgccgga ttggccatag ggtttggagc gcagactctg gtccgcgact tcatcaacgg
     3901 cttcttcatt ctggtggaga accagtacga catcggcgac accattaagc ttgccagcgt
     3961 gcagggtgtg gttgaggaca tgaccttgcg ccgtaccgtg ctgcgcgacg acaacggtag
     4021 cgtgcacacc gtgcccaatg gcgagatccg aattgtgacc aatctcacgc gcgactggac
     4081 gcagttgccc atgcatatct ccgtggccta tggcgccgac agcgacgagg tgatcaaact
     4141 gct
//
LOCUS       DASRKK010000826         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_973719, whole genome shotgun sequence.
ACCESSION   DASRKK010000826 DASRKK010000000
VERSION     DASRKK010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973719"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            880..2139
                     /gene="ltrA"
                     /locus_tag="VFC15_19770"
     CDS             880..2139
                     /gene="ltrA"
                     /locus_tag="VFC15_19770"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM12452.1"
                     /translation="MTSTKSFAISKRMVYEAWKRVKANRGAAGIDAQSLAEFEENLAN
                     NLYALWNRLASGSYFPPPVKEVSIPKRNAGVRILGVPTVGDRVAQTVITRYLEPILDP
                     LFDEDSYGYRPGRSAIDAVRVTRERCWRFDWVLEFDVKGAFDGLDHGLIMKALRKHTD
                     CKWALLYVERWLKAPSMSEAGELRRRDRGTPQGGCVSPLILNLFMHYAFDRWIRREVP
                     HCRFARYADDAVVHCQTERMAHWVKQRLAERFAACGLELHPDKTRIVYCRDSKRQAEY
                     PVTQFTFLGFTFRPRSAKSRAGKLFTSFQPGVSREALKRMRQHIRSWHLPRQSPGTLA
                     EFSAKYGATLRGWWNYYGSFYPSAVWPVFRHFDLALAYWARRKYKRLAGRQRESRHWL
                     AQVARRAPRLFVHWRLWFAIDRTLGAV"
BASE COUNT          505 a          693 c          808 g          526 t
ORIGIN      
        1 aagaacatac aacacaccgt ccgatacacc gagctgagcc cggcgcgatt caagggattc
       61 tggcgggatt gacggggtct acaggtcccg ctctacgcgc cctcaccgtg cctttcgccg
      121 aatgtctcgg ccggtcagcg accgtcaacc gcgatcgact cgtcgatggt cgcttcgtgt
      181 tgaccccaag ccgcctgatg caaccgtctg cttccgagca gcgaaattgc aaaccttccc
      241 ataaacgagc attggcgcag gtgcggcata acgaactcaa acccctacgc aacaaagctt
      301 tcgagctctc ttctgcactc ccatttaagg cccaaaatgg ggtgtgccat gaacacttga
      361 gatggtcaag tgatgctgta gcgaagatga gggtaggccc ctcgaagtcg ccttccagaa
      421 ggagacttca aaccgcccac tgctgctgcg gccgcaagtc cgtggtggtg agcgagtggg
      481 ttaacaggca cgtgttaacc gtgtctgggt atggaccgag gagatgagcg agagcgaacc
      541 gctgatgacg tatcgaaagc cctttgagat gttgtcgaaa ccagggatgc ccatttttcc
      601 tgggatcagc ccgcggggag tctggcgact gcgcgggcgg caaccggtat ataggcggca
      661 tgacctcggt tctggctttg ctatggaaca tgggaacctg tcggtcgatg tcaagggaaa
      721 gcggcgagtg ggtaatacca cgagtgcgaa taccgaggcg atcctcaggg gcggagtggc
      781 tcgcagtagt gatgaagcgc ctgtaacggg cgtggagcga aggggccacg gtgtcttgga
      841 gatgaagtcg atcaaccttc gatgagaagg gaggaatcgg tgacatcgac gaagtcgttt
      901 gcgatatcga aacgcatggt gtacgaggcc tggaaacgcg tgaaggcgaa ccggggtgcg
      961 gcgggtattg atgcgcagag ccttgctgag ttcgaggaga acttggcaaa caatctctac
     1021 gcattgtgga accgtctggc ctcggggagc tattttccac cgccggtcaa agaggtatcg
     1081 ataccgaagc gtaatgctgg agttcgaatc ctcggtgtgc cgactgtggg tgatcgcgtg
     1141 gcccagacgg tcattacgcg ttatctggag ccgatcctgg acccgctgtt tgacgaggac
     1201 tcctacggtt ataggccggg gcggtcagcg atagacgcgg ttcgggtgac gcgcgagcga
     1261 tgttggaggt tcgactgggt gctggagttt gacgtcaaag gagcgtttga cggactcgac
     1321 cacggtctga tcatgaaggc attgaggaag catacggact gcaaatgggc gctgctgtat
     1381 gtcgaacggt ggctgaaggc gccgtcgatg agcgaggcag gagaactcag gcgccgggat
     1441 cgcggcacgc cacagggcgg ctgcgtaagc ccgctgatcc tgaatctctt catgcattat
     1501 gcgttcgatc ggtggatacg gcgggaggta ccgcattgtc ggtttgcccg ctacgccgac
     1561 gacgcggtgg tgcattgcca gactgagcgg atggctcatt gggtcaagca acgattagct
     1621 gaacgctttg cggcgtgcgg tcttgagttg catcctgaca agaccaggat cgtctattgc
     1681 cgggacagta aacggcaagc cgagtatccg gtcacgcagt tcacgttcct cggcttcacg
     1741 ttccggcctc ggagcgcaaa gtcgcgagcg ggcaagctct ttacgagctt ccagcccggt
     1801 gtcagccgcg aggcgctgaa gcgtatgcgt cagcacatcc gcagctggca tcttccacgt
     1861 caatcccccg gtacgctggc cgagttctcg gcgaaatatg gtgcgacgtt gcggggctgg
     1921 tggaactact acggtagctt ctatccgagc gctgtgtggc ctgtgttccg gcactttgat
     1981 ctggcgctcg cctattgggc acgtcggaaa tacaagcgct tggcaggtcg gcagcgcgag
     2041 agccgtcact ggcttgccca agtcgcccgg cgagcgccgc gtctgttcgt gcattggcgt
     2101 ctttggttcg cgatcgatcg gacactggga gccgtgtgag gcgcgagtct cacgcacggt
     2161 tctgcgagcg actgcggggg aagttcctgc ggtctactca ccaaggtaat cgcacgcggg
     2221 gacggtgctg tcagattacg ctggcactag ctggcagggc cgctcgcatt tgacgcagaa
     2281 acacctctag gttctcgatc cccttcccgt agtctgcctt cgtcgccttc atccggggca
     2341 cgccgcggat gcttacccta gagcgattat ctccgcagct tacgactcgg acagacacaa
     2401 tttctccgaa gctcctcata gccattccaa cgtgagcttc catctcaccg gcttgcttgt
     2461 tttcttggga tatccgcagc cttgggatct gcagcagcgc ggcacggctc gcagcaaacg
     2521 ccaccgccgt tg
//
LOCUS       DASRKK010000827         5071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_96006, whole genome shotgun sequence.
ACCESSION   DASRKK010000827 DASRKK010000000
VERSION     DASRKK010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96006"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFC15_19775"
     CDS             complement(<1..337)
                     /locus_tag="VFC15_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12453.1"
                     /translation="MAQKTAFAVFSQKPTLESSAFAAAGRAFQDESGECRASRRSSDA
                     KHASLRDRPSKGDNEFRDFGGKLVRPYYVPIVDRLLLVSFLDHHHMFSACHRQPGNEG
                     TPRIVNHCFG"
     gene            651..1142
                     /locus_tag="VFC15_19780"
     CDS             651..1142
                     /locus_tag="VFC15_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12454.1"
                     /translation="MDISQAVCSIAVVDPGSWSADWAWGLPLIVLTVILHVLGLGLMA
                     EKTLAISGRMFERRHPTVVFVAVVGTATLFATCLHAIEAGIWAAAYRLLGALSDDRSA
                     MLYSLNAITSYGHAALALKDHWQLMGALEALNGWLLFGLTTAFLFGMIERVWTLVGKA
                     GRH"
     gene            complement(1182..2729)
                     /locus_tag="VFC15_19785"
     CDS             complement(1182..2729)
                     /locus_tag="VFC15_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZM12455.1"
                     /translation="MSTSPASSRQALLLLLSCLALFVLVACGKKETAPGTSSEPAKPQ
                     TAALPVSSPDEPPAASGSVPFPAAFGRRTGDLDEMAKNRNIRALVIMNPIGFFYDKGH
                     PRGVMYDALEEFQKFVNQKLKTGTLDVKVTFLPMRPDQIEAALTQGIGDLIAYGIVVT
                     PEREQRLAFSTPIQTDVSQIIVTGENFGPVSTLADLGGKEVYVNPLTSYYGNLQKVNE
                     DLQKQGKPPILIKAADKNLLDDDLVQMVNAGLVPATVTTKQKAALWAQVFDHIHPHPD
                     LAIATGAQLAWVMRKNNPQLKQVVDEFVTSRAVGTSFGNTVLRRYLQNTKWVKDSTSS
                     EEMKKFQANLELFQKYAGEYDFDYLMIAAQGYQESLLDQSRRNPSGAVGIMQVIPKYA
                     AANPINIPSVATADANIHAGVKMLRNIDDKYFNDPNITPLNKTLFVFASYNAGPNRIV
                     RLRKQAADSGLDPNVWFGNVEMVAAKDIGQETVTYVANIYKYYVAYKLAVEQKELKQK
                     AAGLPAK"
     gene            complement(2848..4470)
                     /locus_tag="VFC15_19790"
     CDS             complement(2848..4470)
                     /locus_tag="VFC15_19790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM12456.1"
                     /translation="MDFGAGVRKLTLGALAISSFLLAGGATPSAAQTSKPNIVVIMGD
                     DIGIWNIGAYSRGMMAGRTPNLDKVAAEGMLFTDYYAEASCTAGRANFITGELPIRTG
                     MTTVGQAGAKIGIPAEAVTIATALKSMGYATGQFGKNHLGDRNEYLPTVHGFDEFYGY
                     LYHLDAMEDPCHPNYPPALKDTVGPRHMLHTWATTVDDPTDQPRWGKVGKQKIQDDGE
                     LCPKRMETIDDEILANAMTFIDKAKTDNKPFFLWLNPTRMHVVTHLSPKYEALRNPEN
                     GWSIEEAGMAQLDDIVGATMQKLKDMGVADNTIVVFTTDNGTENFSWPDGGQTPFAGG
                     KGTALEGGFRSPAILWWPGKVPAGKVENGIFSGLDWFPTFVAAAGDPNIAAELQKGKQ
                     LGGTNYKVYLDGYNQMDLITGKGPSARHEIFYFTESTLSAVRINDFKYRFTDQPNGWL
                     GATVKVDWPILVNLRLDPFERTGMPTATGGSLFFKDWFVYEFWRFVFVQQVVGKLAQT
                     AIDFPPMQKGASFNLSQLKEELDKKIAAHASQ"
     gene            complement(4759..>5071)
                     /locus_tag="VFC15_19795"
     CDS             complement(4759..>5071)
                     /locus_tag="VFC15_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12457.1"
                     /translation="VPALYNLTMDPFERYDMIFNGAEGEMNGLRTSAGRYSGEDNGWA
                     GALTGIVLADFDKSIMQYPSIQRAPGGASNDMIPNLQNPKNPLPLLDPKNMPKEVPGS
                     D"
BASE COUNT          976 a         1415 c         1480 g         1200 t
ORIGIN      
        1 caccgaagca gtggttcaca atgcgcggtg tgccttcgtt gccgggttgg cgatggcaag
       61 cggaaaacat gtggtgatga tccaagaagg agaccaaaag caaccgatcg actataggga
      121 cgtagtacgg acgtacgagc ttcccgccaa agtcccggaa ctcattatcc cccttcgagg
      181 gtctatccct aagactggca tgtttcgcgt cagatgaccg gcgcgaagct cggcactcgc
      241 cggattcgtc ttggaaggcc cgtccggcgg cggcgaaggc gcttgattct agcgtcggtt
      301 tttgcgaaaa gacggcaaac gccgtctttt gtgccaccga agttctttga aatgccagct
      361 aagtcctacg gccagaaatg gatacgtata actctataaa gaatcaatgg catgcgaaca
      421 ttttcagccc aactcattga ttctaaacaa cagtgcataa gtaattttat gtgttctagt
      481 cgactagaac accatctgga tgctggtcga ctcggcccca gcagtggatg tcgatttacg
      541 accacgattc cggggcatgc cttactatgc tgtgagcgga gtgcgccgct tttctgctat
      601 ccacggtcag tactccaccg ggatcgagta agcacgcgaa tgaggtttcg atggatattt
      661 cccaagccgt ttgcagcatt gctgtggtcg acccagggag ttggagcgcc gattgggcgt
      721 ggggcctgcc tttgatcgtc ctgaccgtga ttctccacgt attgggcctc gggctgatgg
      781 ccgagaagac ccttgcgatt tcgggccgca tgtttgaacg ccgccatccc accgtggtat
      841 ttgtcgcggt ggttggcacc gcgaccttat ttgccacttg cctgcacgcg atcgaggcgg
      901 gcatctgggc tgccgcctac aggcttctcg gtgctctatc cgacgacagg tccgcgatgc
      961 tctattcgct caatgcgatc accagctacg ggcacgccgc tcttgcactg aaggaccatt
     1021 ggcaactcat gggagcgctt gaagcgctga acggatggtt gctgttcgga ctgacgactg
     1081 cgttcttatt cggcatgatc gagagggtct ggacgctagt gggcaaggca gggcgtcact
     1141 agccttttga gtcaggacct gctgcttggc ggctggcttt cttatttcgc cggaagccct
     1201 gccgcctttt gtttgagctc cttctgttcc acggcgagct tgtatgcgac gtagtacttg
     1261 tagatattgg cgacgtaggt gaccgtctcc tgcccaatgt ctttggccgc aaccatttct
     1321 acgttgccga accagacgtt gggatcaagg cccgaatcgg ctgcctgttt gcgtagcctg
     1381 acgatgcgat tcggcccggc gttgtagctg gcgaacacaa acagcgtctt gttgagcgga
     1441 gtaatgttgg ggtcgttgaa gtatttgtcg tcgatgtttc gcaacatttt gaccccggca
     1501 tgaatgttgg catcagccgt tgccacgctc ggaatgttga tgggatttgc cgccgcgtac
     1561 ttggggatca cctgcatgat tcctaccgcg ccgctggggt tccttcgact ctggtccagc
     1621 aaagactcct gatagccctg ggcggcgatc atcaggtaat cgaagtcgta ctccccggca
     1681 tacttctgga agagctcaag gtttgcctga aacttcttca tctcttccga ggaagttgag
     1741 tccttcaccc acttggtgtt ttgcaggtag cgccgcagca cggtgttgcc gaacgacgtt
     1801 cccacagcgc gggaggtgac gaattcgtcg actacctgct tgagttgcgg gttgttcttg
     1861 cgcatgaccc acgccagttg ggctccagtg gcgatggcga ggtccgggtg cggatgaata
     1921 tgatcgaaca cttgggccca gagggcggct ttctgcttcg tggtgacggt cgccgggacc
     1981 aatccggcat tcaccatctg caccaagtcg tcgtccaaca ggttcttgtc ggccgccttg
     2041 atgaggatgg gcggcttgcc ttgcttctgc agatcctcgt tcaccttctg caggtttccg
     2101 tagtagcttg tgaggggatt gacgtacact tcttttccgc ccaggtcggc gagggtggaa
     2161 accgggccaa aattttctcc cgtaacaatg atctgggaga catccgtctg tatcggcgtc
     2221 gagaaagcaa gcctctgttc gcgctccggc gtgaccacga ttccgtaggc aatcaagtcg
     2281 cctatgcctt gtgtcagggc cgcttcaatc tggtcaggac gcatgggaag gaacgttact
     2341 ttcacatcga gcgtgccggt cttcagcttc tggttgacga acttctggaa ctcctccagc
     2401 gcatcgtaca tcacgcctct tggatggccc ttgtcgtaga agaaaccgat agggttcatt
     2461 atgacgagtg cccggatgtt gcggttcttc gccatctcat cgagatcgcc ggtgcggcgc
     2521 ccgaatgccg ccgggaacgg gacgctgccc gacgccgcgg ggggctcatc gggcgacgat
     2581 accgggagcg cagcggtctg aggcttggct ggttcgctgc tggtcccagg cgcagtttcc
     2641 ttcttgccgc aggccaccag cacgaaaaga gccaggcaag atagcagcaa cagcaaagct
     2701 tggcgggacg aggccggcga tgtagacatt catcttctcc gctgcaaaca aatttagggg
     2761 gtgtgcaatt gtcgcacacc ccatctttat tctgcctgca gcaaacgggg cggctcctga
     2821 ggaccgcccg ccgggttgag tgtttgctta ctgagacgcg tgcgccgcaa ttttcttgtc
     2881 gagctcttct ttcagttgtg acaaattaaa gctcgcgccc ttttgcatcg gcgggaagtc
     2941 gatcgcggtc tgagcgagtt tccccaccac ctgctggacg aacacgaagc gccagaattc
     3001 gtacacaaac cagtctttga agaataggga cccgccggtt gcggtcggca tccccgtgcg
     3061 ctcgaatgga tcaagacgca gattgaccag gatcggccaa tccactttga cggttgcacc
     3121 caaccagccg ttcggctgat cggtgaagcg atacttgaag tcattaattc ggaccgccga
     3181 aagcgtgctc tcggtaaagt agaaaatctc gtgccgcgca gatgggcctt ttccggtgat
     3241 caggtccatc tggttgtaac cgtcgagata caccttgtaa ttcgtaccgc cgagttgttt
     3301 gcccttctgc aattcagcag cgatgttggg atcgcccgca gcagctacga acgtgggaaa
     3361 ccagtcgagt ccggagaata ttccgttctc aacttttccg gcaggcacct tgcccggcca
     3421 ccaaagaatc gctggtgagc ggaagccacc ttccagcgcc gtacccttgc cgccagcgaa
     3481 cggggtctgt ccgccgtctg gccaggagaa gttctcagtg ccgttgtcag tggtgaagac
     3541 gacgatggtg ttgtcggcga cgcccatgtc cttcagcttc tgcatggtcg cgcccacgat
     3601 atcgtccagt tgcgccattc ccgcttcctc gatagaccag ccattctcgg gattgcgcag
     3661 ggcctcgtac ttcggcgaca agtgcgttac tacgtgcatg cgcgtgggat tgagccacag
     3721 gaagaacggc ttgttgtcgg tcttcgcctt gtcgatgaac gtcatcgcat tcgccaggat
     3781 ttcgtcatcg atggtttcca tgcgtttggg acaaagttcc ccgtcgtcct gaatcttttg
     3841 cttgccgacc ttgccccagc gtggctggtc cgttgggtca tcgacagtgg tcgcccaggt
     3901 atgcaacatg tgccgaggcc cgacggtgtc ctttagcgcg ggaggatagt tgggatggca
     3961 ggggtcttcc atcgcatcca gatggtagag gtaaccgtag aactcgtcga acccgtggac
     4021 ggtaggcagg tattcattgc ggtcgcccag gtggttcttg ccgaactggc ccgttgcgta
     4081 gcccatggac ttgagtgccg tcgcgatcgt cactgcttcc gcgggaattc cgattttggc
     4141 gccggcctgg ccgaccgtcg tcatgccggt gcggatcgga agctcgccgg tgatgaagtt
     4201 ggcgcggccc gccgtgcaac tcgcttccgc gtagtagtcg gtgaaaagca tgccttcagc
     4261 agcgaccttg tcgagattgg gagtgcgccc ggccatcatg ccgcggctat aagcaccgat
     4321 gttccaaatg ccaatgtcgt cgcccatgat cacgacaatg ttgggcttgc tcgtttgcgc
     4381 cgcgcttggg gttgcaccac ccgccagcaa aaagctggag atggcaagcg cgcccagcgt
     4441 tagttttcga actcctgcac caaaatccat tgtgaatctc ctgttgcaag tactgcgaga
     4501 tagtctccat gaggagggtt acgatgagtg agcgtcagcc catcagaggt ggctatcgac
     4561 agtgcgctaa aacacgccta aacgctacac caattctcgt agtttgcgaa atatatccct
     4621 gctctcattt cagtgagatt acggacccca cgtcaccacc gagtgcgaag tagccgtcga
     4681 ttgccgcgtc gtccggagat agaacaaggg gcatgcaatg tcgcacgccc cttgggttgt
     4741 tgtggccgaa gccgtgagtt agtcgctccc aggaacctcc ttcggcatgt ttttgggatc
     4801 cagtagcggc agcgggttct taggattctg caagttcgga atcatgtcgt tcgacgcgcc
     4861 acccggtgcg cgctggatgc tcgggtattg catgatcgac ttgtcaaagt cggccagcac
     4921 aatgcccgtg agcgcacctg cccagccgtt gtcttcgccg gaataccgtc ccgccgaggt
     4981 ccgtaatcca ttcatttcac cttcggcgcc gttgaagatc atgtcgtatc tctcgaacgg
     5041 atccatggtg aggttgtaca gggcgggaac a
//
LOCUS       DASRKK010000828         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_289299, whole genome shotgun sequence.
ACCESSION   DASRKK010000828 DASRKK010000000
VERSION     DASRKK010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289299"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFC15_19800"
     CDS             <1..963
                     /locus_tag="VFC15_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM12458.1"
                     /translation="DRIVLCLPLHHVHGIVNVVSCALWSGATCEMLPRFDANAVWECI
                     AGSSITLFMAVPTVYVKLIAVWDAASPERRATLSEACAKLRLMVSGSAALPVSTLERW
                     KEISGHTLLERYGMTEIGMALSNPLRGERVPGSVGTPLPSVEVQLVSENGAPVAPGTP
                     GEIEVRGPSVFAEYWGKPDAMRDAFRDGWFRTGDTAVVENGVYRILGRTNIDILKTGG
                     NKVSALEIEETLREHPSVAECAVVGVPDPEWGQRVAAAVVLKDGGALDLPSLRIWAKE
                     FLAAHKLPSRLLVLDALPRNAMGKVTKPAIATMFRAVGTGDPVA"
     gene            complement(1010..1441)
                     /locus_tag="VFC15_19805"
     CDS             complement(1010..1441)
                     /locus_tag="VFC15_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12459.1"
                     /translation="MIGSFDTSGCPTIKIKVSGITGKPTEFTALVDTGFSGFLSLPML
                     SAFPLGLVLSGTMNITLADGSTHVRLTCRGMVEIDGSKKIGLITLETGASDVLVGMEF
                     IAKFNKQLVVCPKNSSIELIDAPAPIPKPVPTDQPNPPPAP"
     gene            complement(1425..1697)
                     /locus_tag="VFC15_19810"
     CDS             complement(1425..1697)
                     /locus_tag="VFC15_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12460.1"
                     /translation="MNRSSPKEMAAAGEAIYNEKYRTAFEAEHPGKFVAIDINTGKAW
                     VNDTPEGALGVAQGECPDGMFHLIKVGSPGVYRIGYSQQASCDWFV"
     gene            2070..3374
                     /locus_tag="VFC15_19815"
     CDS             2070..3374
                     /locus_tag="VFC15_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12461.1"
                     /translation="MANDAVALPRLRVHTKLNRHQITGFWGAWAGWTLDGMDSVIYAL
                     VLSPALTELLPKSGIKATPATVGYAGSVLFALFLVGWGLSLIWGPIADRFGRTRVLAA
                     TIFVYAIFTAAAAMSQTVWQLGLFRLLAGIGIGGEWALAGTYVAEAWPEDRRKMGAGY
                     LQTGYYAGFFLASALNYTVGAHYGWRAMFWCGLTPVVVACMVLLRVKEPQRWQQKAKA
                     KAAGGISSLHRIFSPPYTQRTLVNTVLLAAAICGLWAGAVYAPTAIIALAKRAGMLQP
                     QAVRMSSFGMGLLSLGTILGCLAVPPLAERFGRKRTLAFYFVGMAICIVLSFGWAFHL
                     PQGLHPFIAVLFFLGFFGGNFAVFSLWLPEQYGTTVRATAFAFTTSFGRFIAAGVNLG
                     IGALVMRMGTPGKPVAFTAIAFGIGLLVIPFAVETRGKVLPD"
BASE COUNT          652 a         1019 c         1076 g          788 t
ORIGIN      
        1 gatcgcatcg tgctgtgctt gccgctgcac cacgtgcacg ggatcgtcaa cgttgtttct
       61 tgtgcgctgt ggtccggcgc gacctgcgag atgctgccgc gcttcgacgc gaatgccgtt
      121 tgggagtgca ttgccggcag cagcataact ctcttcatgg cggtgccgac agtttacgtg
      181 aaactcattg ctgtctggga cgcggcttcg ccggagcgcc gcgcgaccct cagtgaggcg
      241 tgcgccaagt tgcgcttgat ggtgtccggc tcggcggcgc tgccggtgag caccctcgaa
      301 cgctggaagg agatcagcgg ccacaccctg ctggaacgct acggcatgac cgagattggc
      361 atggccctgt cgaacccgtt gcggggcgag cgcgtgccgg gcagcgtggg gacgccgctg
      421 ccgagcgtcg aggtacaact tgttagcgag aacggcgcgc cggtcgcgcc gggaacgccg
      481 ggcgagatcg aagtccgcgg tcccagcgtg ttcgcggaat actggggcaa gcccgacgcg
      541 atgcgcgatg ccttccgcga tggctggttt cgtactggcg atactgcggt cgtcgagaac
      601 ggtgtttatc gcatcctcgg ccggacgaac attgacatcc tcaaaacggg cgggaacaag
      661 gtctccgcgc tggagattga ggaaacgctg cgcgagcatc cttccgtcgc cgagtgcgcc
      721 gtggtcggag tgcccgatcc ggagtggggc cagcgcgtcg ccgcggcggt ggtgctaaag
      781 gatggcggtg cgctcgatct gccgtccctt cgcatttggg ccaaggaatt tcttgcggcc
      841 cacaaactgc cgtcacggct tctcgtgctg gacgcgcttc cgcgcaatgc gatgggaaaa
      901 gtaaccaagc ccgcgatcgc gacgatgttt cgggccgtgg gcaccggcga tcccgtcgcc
      961 taggacctta tgacctgtta ggggatcagt ttgaatcagc gggagcgatt tacggagcag
     1021 gaggtggatt gggttgatcg gtaggaaccg gtttgggaat gggggctggc gcgtctatga
     1081 gttcaatcga ggagttcttc ggacacacga cgagctgctt gttgaacttc gcaatgaact
     1141 ccatgccgac aagtacatcg gatgctccgg tttccagtgt aatgagccca attttcttgc
     1201 tcccatctat ctcgaccatt ccccgacacg taaggcggac atgggtactc ccatcagcga
     1261 gcgtgatatt catcgtaccg gagagtacca gccccaaggg aaaagctgac agcatcggca
     1321 gggacaaaaa tccgctgaaa cccgtatcga ccaaagccgt gaactcggtt ggcttcccgg
     1381 ttattccact taccttgatc ttgattgttg ggcagccgga ggtatcaaac gaaccaatca
     1441 catgacgcct gctgtgagta accgatcctg taaaccccag gggaccccac cttgatgaga
     1501 tggaacatcc cgtcggggca ctcgccttgt gcaactccca atgctccttc gggagtgtcg
     1561 tttacccacg cctttcctgt gtttatgtcg attgcgacaa acttaccggg gtgttcggcc
     1621 tcgaacgcag tacgatactt ctcgttatag atagcctcac ccgccgcagc catttctttg
     1681 ggagatgaac gattcacggg catatgcttt gtttgatgag atgacgccat aagcggatac
     1741 ctcaattgaa gcacgaatcg tacatcggcg aactgccgtt tgcaaaatat tatttttgga
     1801 ttcccaaaat gagagataca agttcatcga agccccaggt ggatctttct gaatctttcc
     1861 atgagcgctc aggggccttc aaagtgcacc actccccatg gcctcctgta cttgacatgc
     1921 cgtggtatgc tgcccacgcc caataaatct gggcgcatcg tggcgtgagc gcttgcggtt
     1981 cggatcatag atgcgggttc ttttcgcagc tcccgaatct caagtagcgg tgtctttcgc
     2041 gctcactccc tcaccgcaag gaggacacct tggccaacga cgcagtggcc cttccacgac
     2101 taagagttca taccaaactc aaccgccatc aaatcaccgg attctggggc gcatgggctg
     2161 gttggacgct cgacggcatg gattcggtga tctatgcact cgtcctcagt ccagccctga
     2221 ccgaactgct tccgaagtcg ggcatcaaag ccacgccggc gacggtcggg tacgctggat
     2281 ctgtcttgtt cgcactcttc ctggtgggat ggggactgtc gttgatttgg ggcccgattg
     2341 ccgacaggtt tggccggacg cgagtgctcg ctgccaccat cttcgtttac gcaatcttca
     2401 ctgccgccgc cgcgatgtcg caaacggtgt ggcaactggg attgttccgg ttgctggcgg
     2461 gcatcggaat tggcggcgaa tgggcgctgg cgggcacgta cgtcgccgaa gcctggccgg
     2521 aagaccgccg caagatgggc gcgggatatt tgcagactgg ttattacgcg ggcttctttc
     2581 tggcctcggc cctcaactac acggtgggag cgcactatgg ctggcgcgcc atgttctggt
     2641 gcgggctgac gccggtcgtc gtggcctgca tggttctgct gcgcgtcaag gagccccagc
     2701 gctggcaaca aaaagccaag gcgaaggccg ccggagggat ctcttctctg cacaggatct
     2761 tcagtcctcc ctacacgcag cgcacgctgg tcaacaccgt tctgctggcc gcggccatct
     2821 gcggcctgtg ggcaggcgcc gtttatgcgc cgacggcaat tattgctctg gcgaagcgtg
     2881 ccggcatgtt gcagccgcag gccgtgcgca tgtcttcttt cggcatggga ctgctgtcgc
     2941 tggggaccat cctcggatgc ctggcggtcc ccccactggc cgagcgcttc ggccggaagc
     3001 gcacactagc cttttacttt gtgggcatgg cgatctgcat tgtgctcagc tttggctggg
     3061 cgttccactt gccccaggga cttcatccgt tcatcgcggt acttttcttt ctgggcttct
     3121 ttggcggcaa cttcgccgtc ttcagtctct ggctgccgga gcagtacggg accacggtac
     3181 gcgccacagc tttcgcgttc accacttcgt tcggacgctt cattgctgcg ggtgtcaacc
     3241 tgggaattgg cgctctggtc atgcgaatgg gaactcccgg caagccagtg gcattcaccg
     3301 cgattgcttt tgggatcgga ctgctggtta ttcctttcgc agtggaaacc cgcggcaaag
     3361 tgctacctga ttaagaacgg tgcttgcggt gcacccatgg aaataaaaat ttaccacgga
     3421 ggtacggaga tcgccgagag aagactcgcg agcaactgaa actccgtgcc ctccgtgtct
     3481 ccgtggtgaa tgatttttcg atgaagggga atactcgtcc tgtgtgtggc agcta
//
LOCUS       DASRKK010000829         9776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_780856, whole genome shotgun sequence.
ACCESSION   DASRKK010000829 DASRKK010000000
VERSION     DASRKK010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780856"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1146)
                     /locus_tag="VFC15_19820"
     CDS             complement(67..1146)
                     /locus_tag="VFC15_19820"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZM12462.1"
                     /translation="MPSEKQVKWSQLRVGLTVIIASITLAVLIFVMTGTTGLFTRKIL
                     IRAYVDNAGGLRVGAPVRLEGVDIGNVTHIRVVHDPLRKFAPVEVTMKITTKYEDTMR
                     SDCHASMNTAGVLGEVFVDIDCRQAKGGPLVNGAELPTDDVTQLQDVVKASQGTLQNV
                     DTLVKRLDNILTFIQSGQGSIGKVIYDPALFNRANDMLTQLQQIVAQANSTNGSIGKL
                     LNSDELYNKANLAVDNLNKTIDQINSGQGTIGKFIKDPALYDNANQTMAKANQLITDI
                     NAGKGTLGKLAKDEELAKKLDITVTRLNSILDRLDKGEGTAGKLMVDPSLYNNADATL
                     ASAHQLIEAIKKDPKTYLTIHLKIF"
     gene            complement(1357..4107)
                     /locus_tag="VFC15_19825"
     CDS             complement(1357..4107)
                     /locus_tag="VFC15_19825"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HZM12463.1"
                     /translation="MTAAFKPNKPIGKADPSSDVYRSESHPLDPLFAPSSVAVIGATE
                     RVGSVGRSVLWNLLSSPFGGTVFPVNSKRPNVLGIKAYPKVSDLPDKVDLVVVTTPAD
                     TVPDLLAESVEMGIPAGIVISAGFKEHGEHGKELERQITKIIRGKMRLIGPNCLGVMN
                     PIRGLNATFAHAIARPGNVAFISQSGALLTAILDWSLRENVGFSSFVSTGSMLDVNWG
                     DLIDYFGSDPRTHSIVIYMESIGDASAFLSAAREVSLTKPIIVIKAGRTAAAAKAAAS
                     HTGSLTGSDEVFDAAFRRSGVLRVTSISDIFFMADVLAKQPRPRGKRLAILTNAGGPG
                     VLATDALVTGGGELAELSPVTMAAFDEILPPEWSHNNPVDILGDAQPERYAKSLEIAA
                     KDPAIDGMLVILTPQDMTNPTLIAEKLKPYAKGFGKPVLASWMGGAEVAAGEQILNQA
                     GIPTFQFPDSAVRAFNYMWRYAYNLKGIYETPTLPHHEEAAVQRGKAERIIRDARQQG
                     HTILTEYESKELLKAYGIPTVETRIAVTEDEAVQAAQEIGFPAVVKLYSMTITHKTDV
                     GGVLLNLRDADAVRKGFQAIKAAVAEKAGAEHFQGVTVQPMVRLDGYELIIGSSVDSQ
                     FGPVMLFGTGGQLVEVFKDRALALPPLNSTLARRMMEQTKIFTALKGVRGRKPVDIEA
                     LEELLVRFSQLIVEQPWIREIDINPLLASPEQLLALDARVVVYGPEVKADELPESAVR
                     PYPVRLVSNWKMKDGKEVVIRPIRPEDEPLMITFHQKLSERSVYLRYFQPLKLTQRTA
                     HERLTRLCFIDYNREMALVAERKNEAGETELLAIGRLSKIHGRDEAELAAVAIDASQH
                     KGLGTELYTRLIAVGREAKLKKLISNMLPENREMRALCVKLGFKMHSSLEDNMIRAEL
                     DL"
     gene            4323..5375
                     /gene="purM"
                     /locus_tag="VFC15_19830"
     CDS             4323..5375
                     /gene="purM"
                     /locus_tag="VFC15_19830"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZM12464.1"
                     /translation="MAQHTTEDRSITYADAGVDIERANRTKQRIKYLAHKTFNRGVLS
                     EIGGFGGLFAIDKKKYLDPVLVSSVDGVGTKLKIAFEMGLHHTIGADLVNHCVNDIAV
                     QGAAPMFFMDYFATGKLHSEVVEKVISGIADACKQNACALIGGETAEMPGFYPDGEYD
                     LAGFIVGVVDRPKIVTGKDVQVGDVLIGIPSTGLHTNGYSLARKLFFEIARYTPDSYV
                     NEIKGKVGTELMRTHKSYWPIMRRMIDAEAVSAMAHITGGGITDNLPRVLPKGMGAIV
                     EFHSWPVLPIFQHLQAIGNVPQDDMLRTFNMGIGMVLVVPAKKFKKAQTILERCGEKG
                     YTIGRIVKGERRVTYQ"
     gene            5643..6287
                     /locus_tag="VFC15_19835"
     CDS             5643..6287
                     /locus_tag="VFC15_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12465.1"
                     /translation="MPTRNAGQQRRPKQAGDYVFLNLPYDAKFEPLYLAYIAGVSAFG
                     LLPRTPLEIPGGSRRLDNIFDQLRGCRYSIHDLSRVQVTRTAPRTPRFNMPFELGLAV
                     AHQKAGGDHTWFVFEQLAHRLSKSLSDLDGSEVYLHDGKPNGILAALSNAFIRSDRQP
                     TVDQMLAIYRELRSAKSTILHDAGAKTLFAARPFKDLSTLASALADGVILGSSR"
     gene            6398..7891
                     /locus_tag="VFC15_19840"
     CDS             6398..7891
                     /locus_tag="VFC15_19840"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HZM12466.1"
                     /translation="MERLVAILALFLSAPFLFAADLSGIWLYSAKQPDGHTARMVLAL
                     QQDGDNLAGKIEHPWGTLNIKEGKVDGNRFTITAPISDGRPYICEGTLEGDKLHFTVR
                     DPNAKPYEVVAVRSPSDPFRVTTPLTPPAVRDLPGNGLAKTPPMGWNSWNLFAGRIDD
                     KIVREMADAMVSSGMRDAGFIYLNIDDTWEGDRDANGVIHSNNKFPDMKALADYVHSK
                     GLKFGIYSSPGPYTCAGYEGSYAHEAQDAKTYAAWGVDYLKYDWCSAGGLYPDSAMRA
                     VYQKMAEALRATNRPIVYSLCQYGKENVWEWGAEAGGNLWRTTDDINDTYKRMMEIVD
                     IQSAIAKWAGPGHWNDPDMLEVGNGGMTPDEYRTHMSLWAMFAAPLLAGNDVRAMSAD
                     TRAILLNKDVIAIDQDPLGQQGQLALKRSDVDYWKRQLSGGGVAIAAVNRSSSPVAVN
                     ILWRELKVDGNLVFKDVWSGMQIKGSSDSTLTIQAHGVVLLRSELVH"
     gene            complement(8052..8819)
                     /locus_tag="VFC15_19845"
     CDS             complement(8052..8819)
                     /locus_tag="VFC15_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12467.1"
                     /translation="MAFLDAAMEMEVKATEGGTKVNKKLGWLIFMLALALCVSALPAM
                     AQTLFSDLGPPGDQYNCCQGATVSGSGSITGFSDIDASRFTVNGSGPQIVTQIDMGVT
                     FIESLHTFYTSIWTDNGGLPGSQVPNAFWSPLTTITEFGFCPPRCPGDYDGGGFVTIH
                     GISGVTLIGGQSYFMIVGPVALDDASLNAWNLNSQGQTGLTLFSTDDGAMWNSRGYGS
                     TLDAFDILGTPEPSCLLLLGTGFMGALGAIRFKKLAK"
     gene            complement(9064..9585)
                     /locus_tag="VFC15_19850"
     CDS             complement(9064..9585)
                     /locus_tag="VFC15_19850"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZM12468.1"
                     /translation="MTTRFLERQKLKLALCGCLALLLLSTACNQAPPDTRAADEAAIR
                     DTDAQWAKTAGANDLEGTVAYYTDDASLLAPNVPIATGKQAIHAAWAGLLGPGTSISW
                     QATKVEVARSSDLASIMGTYQLTMKDPQGNPVTDKGKFVEVWKKQADGKWKVVADIFN
                     SDVPLPAPVEVKK"
BASE COUNT         1974 a         2944 c         2809 g         2049 t
ORIGIN      
        1 ctactacaaa cgccttagac tgtgaaattc gcgggaggtg agcagcgaac ctcgtggcta
       61 ctgagattag aagatcttca ggtggatcgt caggtatgtc ttgggatctt tcttaatcgc
      121 ctcgatcaac tggtgggccg aagcgagggt ggcatcggca ttgttgtaga gcgagggatc
      181 gaccatcagc ttgcctgcag tgccttcacc cttatccaac cggtcgagaa tggaattcaa
      241 ccgcgtcacc gtgatgtcga gtttcttcgc caactcctcg tctttggcca gcttgccgag
      301 cgtccccttg ccggcgttga tgtcggtaat caactggttg gccttcgcca tggtctggtt
      361 ggcgttgtca taaagtgccg gatctttgat gaacttgccg atcgtgccct gtccgctgtt
      421 gatctggtcg atggtcttgt tcagattgtc gactgccaga ttggccttgt tataaagctc
      481 gtcgctgttc agcagcttgc caatgctgcc gttggtggag ttcgcctgcg ccacgatctg
      541 ctgcaactgg gtcaacatgt cgttggcgcg attgaacagc gccgggtcgt aaatgacctt
      601 gccaatcgat ccttgcccgc tctggatgaa ggtcaagatg ttgtccaggc gtttcaccag
      661 ggtgtcgacg ttttgcagcg tcccctggct ggccttcacc acgtcctgca attgggtgac
      721 atcatccgtg ggcagctcgg cgccgttcac cagcggaccg cctttggcct ggcggcagtc
      781 gatatccacg aagacctcgc ccagcacgcc cgcggtgttc atcgaagcgt gacagtcgga
      841 ccgcatggtg tcctcgtact tggtggtgat cttcatggtg acttcgacgg gggcgaactt
      901 gcggaggggg tcgtgcacta cacggatgtg ggtgacgttt ccgatgtcaa cgccctccag
      961 ccgcactggg gcgccgacgc gcaggccgcc ggcattgtca acataggcgc gaatgagaat
     1021 tttcctggtg aacaggccgg tagtgccggt catcacgaag atcaggacgg ccagcgtgat
     1081 gctggcgatg atgacggtca gcccaacccg gagttgcgac catttcacct gtttttcgct
     1141 cggcacgttc taacgatcgc ccctttggca ccgcgaatcg agcagcgcaa acgccgtgcg
     1201 ggattggact ccggctggca ctggcatcat agcattaggt tgtcgccagc gtcacgattg
     1261 cgggccgcgt ggagccgctc cgtagggcac caccctaata aacaaggcac caggcatcac
     1321 tgcccggcgc cttccgtgaa ttcagcttcg aaaaactcac aggtccagtt cggcccggat
     1381 catgttgtcc tcgaggctgg agtgcatctt gaagcccagc ttgacgcaca gcgctcgcat
     1441 ctcgcggttc tcgggaagca tattcgagat cagcttcttc agcttcgctt cgcgccccac
     1501 ggcgatcagg cgagtataga gctctgtccc caagcccttg tgctgggatg cgtcgatggc
     1561 caccgcagcc agctcggctt cgtcccggcc atgaattttg ctcaagcgac cgatcgccag
     1621 caactccgtc tcgcccgctt cattcttgcg ctcggctacc agcgccatct cgcggttgta
     1681 gtcgatgaag cagagccgcg tcagcctttc gtgcgcggtg cgctgggtca acttcagcgg
     1741 ctggaagtag cgaagataga cgctcctctc cgacagcttt tggtggaacg tgatcatcag
     1801 tggctcgtcc tccggacgga tgggccggat cacgacctcc ttgccatcct tcatcttcca
     1861 gtttgagacc agccggaccg ggtaggggcg gactgccgac tcgggcagtt cgtctgcctt
     1921 cacctcaggg ccatacacca caactcgggc atccagcgcc agcagttgct ccggcgaggc
     1981 cagcaacgga ttgatgtcga tctccctaat ccacggctgc tccacgatca actggctgaa
     2041 gcgcaccagc agttcttcca gagcttcgat gtccaccggc ttgcgtccgc gcacgccctt
     2101 caacgcagtg aagattttgg tttgttccat catccggcgg gccagggtgg aattcagcgg
     2161 aggcagcgcc agcgcccggt ccttgaacac ttccaccagt tgtccgccag taccgaagag
     2221 catgaccggc ccaaattgcg agtccacgct gctgccgatg atcagctcgt agccgtcgag
     2281 cctgaccatg ggctgcacgg tgactccctg gaaatgttct gcgcctgcct tctcggcgac
     2341 agcggctttg atcgcctgga agcccttcct taccgcgtct gcatcgcgca ggttcagcag
     2401 cacaccgccg acatcggtct tgtgggtgat ggtcatcgag tacagcttga cgaccgccgg
     2461 aaagccaatt tcctgcgccg cctgcaccgc ctcgtcctcg gtgacggcaa tccgcgtctc
     2521 caccgtcgga atgccgtagg ccttcaacaa ctccttcgac tcgtactcgg tgaggatggt
     2581 gtggccctgc tggcgcgcat cgcggataat gcgctccgcc ttgccccgtt gcaccgccgc
     2641 ctcttcatgg tggggcaacg tgggcgtctc atagattccc ttcaggttgt acgcgtagcg
     2701 ccacatgtag ttgaaagcgc gcaccgcgct gtcaggaaac tgaaacgtcg ggatgccagc
     2761 ctggttcagg atttgttcgc cggccgcaac ctccgcgccg cccatccagc tggccagcac
     2821 cggcttgccg aaacccttgg cgtagggctt cagcttttcg gcgatcagcg tggggttggt
     2881 catgtcctgg ggagtgagga tcaccagcat gccgtcgatt gcgggatctt tcgcggcgat
     2941 ctccagcgat ttggcgtaac gttctggctg agcgtcgccc aggatgtcta ccggattgtt
     3001 gtggctccat tccggcggca ggatctcgtc gaaggccgcc atggttacgg gcgaaagctc
     3061 ggcaagctcg cctccgcctg taaccagagc atcggtggcc agcacgccgg gtccgccagc
     3121 attggtgagg atggccagcc gtttgccgcg cggacgcggt tgcttggcca gcacgtcggc
     3181 catgaagaaa atgtcagaga tgctggtgac ccgcaacaca ccgctgcgcc ggaatgccgc
     3241 atcgaacacc tcgtcgcttc cggtcaggga cccggtatgc gaagccgcgg ccttggcggc
     3301 ggcggcggtg cgtccggctt tgatcacgat gatgggcttg gtaagcgaaa cctcgcgcgc
     3361 cgccgacagg aaggcgctgg cgtcgccgat cgactccatg tagatgacga tgctgtgagt
     3421 acggggatcg ctgccgaagt agtcgatcag gtcgccccag ttcacatcca gcatcgagcc
     3481 ggtggacaca aacgagctga agcctacgtt ctcacgcaag ctccagtcga gaatggccgt
     3541 gagcagcgcg ccgctctggc tgatgaaggc cacgttgccc ggtcgcgcga tggcgtgggc
     3601 gaaggtggcg ttcaggcccc ggatcggatt catgaccccc aggcagttgg ggccgatcag
     3661 ccgcatcttg ccgcggatga ttttcgtgat ttgcctctcc aactccttgc cgtgctcgcc
     3721 gtgctccttg aatcctgccg agatcacgat accggcggga attcccattt ccaccgattc
     3781 cgccagcagg tctggaaccg tatcggcggg cgtggtcacc accaccaggt caaccttgtc
     3841 aggcaaatcg gaaaccttgg gatatgcctt gattcccaac acgttggggc gttttgagtt
     3901 caccgggaag acggtcccgc cgaaggggct gctgagcagg ttccacagca cggaacggcc
     3961 aacactgcca acccgctcgg tggcgccaat cacagcaaca ctgctgggag caaataatgg
     4021 atcaagagga tggctttcag accggtaaac gtcggaggag gggtcggcct tgccgatggg
     4081 cttgttgggt ttgaatgcag cagtcatgga attcctcaaa ttaggggtat gaaggtctag
     4141 tgtagtccca gccagatttt caagtcatgg tcaggtcacg gcgcggaagc gatttggccc
     4201 accgttgatc gtggtgacaa agtctgcgac tccatcgcta agcaattgca cagagaatgg
     4261 tacaatcact ttttcctgca cgagacctgc ggcgacaagc cctctggatg ggatcccgac
     4321 tcttggcaca gcatacgact gaagaccgct ccatcaccta cgccgatgcc ggggtggaca
     4381 ttgaacgcgc caatcgcacc aagcagcgca tcaagtacct cgcccacaag acctttaacc
     4441 gtggcgtcct gagcgagatc ggtggtttcg gcgggctctt tgccatcgac aaaaaaaagt
     4501 acctcgatcc cgtcctcgtc tccagcgtgg atggtgtcgg cacgaagctc aaaatcgcct
     4561 tcgaaatggg cctgcaccat accatcggcg ctgacctggt caatcactgc gtcaacgaca
     4621 tcgccgtgca gggcgccgca cccatgttct tcatggacta cttcgcgact ggcaagctgc
     4681 attccgaagt ggttgaaaag gtgatcagcg gaatcgctga cgcctgcaag caaaatgcct
     4741 gtgccctgat cggcggcgaa accgccgaga tgccgggctt ttatccggac ggcgagtacg
     4801 acctggcggg attcatcgtt ggcgtggtgg atcgccccaa aatcgtcacc ggcaaggacg
     4861 tgcaggtcgg cgacgtgctg atcgggattc cctcgaccgg acttcatacc aatggctatt
     4921 ccctcgcccg caagttgttt ttcgaaattg cccgctacac ccccgattcc tatgtcaacg
     4981 agatcaaggg caaggtcggc accgagttga tgcggaccca caaaagctac tggcccatca
     5041 tgcgccgcat gatcgatgcc gaagcggtga gtgccatggc ccacattacc ggcggcggca
     5101 tcaccgataa cctgccgcgc gtgctgccca aaggcatggg cgcgatcgtg gagttccact
     5161 cctggccggt gctcccgatc ttccaacatc tgcaggccat tggcaacgtt cctcaggacg
     5221 acatgctacg cacctttaac atgggcatcg gcatggttct ggtcgtgccc gccaagaaat
     5281 tcaagaaggc gcaaaccatc ctcgagcgct gcggcgaaaa gggctacacc atcggccgca
     5341 tcgtcaaggg ggagaggcgc gtgacgtacc aatagccgat gctgttggca tctaaacaaa
     5401 aagcgactat tcttaagcca ctatggcaag gcaagtgaag ctcgcgggga aatttgcaac
     5461 cgccgaagat acggcgaggg cgctcggagt gcctgattcg cgtttggatg aactgaaggc
     5521 cctggcgctc agtatgcttt ccaagcgcgc ccggaagact gcgccgaacg gcgccaagcc
     5581 ccctgcctct gctccgggcg caaagctggc cgtgaaatcc cggaccaaga agagttcccg
     5641 gaatgcccac acgaaacgca gggcaacagc gacgtcctaa acaggctggc gattacgtct
     5701 tcctaaacct gccctacgac gcaaaatttg agccgttgta tcttgcctac atagcgggcg
     5761 tcagtgcttt cggcttgcta ccacgcaccc cacttgaaat tcctggcggt agtcgccggc
     5821 tggacaatat tttcgatcag cttcgcggtt gccgctattc catccacgat ctctcgcggg
     5881 ttcaggtgac tcgaaccgct cctcggactc ccaggttcaa catgccgttt gaattgggac
     5941 tggcggtagc tcaccagaaa gcaggcggcg atcatacctg gtttgtcttt gaacagcttg
     6001 cgcatcggct gtcgaaatcg ctctcggact tggacggttc tgaagtgtac ctccacgatg
     6061 gcaagccgaa cggtattctt gccgcactca gcaatgcctt tataagatcg gaccggcagc
     6121 ccacagtgga ccaaatgctc gccatctatc gcgagctgag aagtgccaaa tcgaccattc
     6181 tgcatgacgc tggtgcgaag acgctgttcg ccgctcgacc cttcaaagac ctttccaccc
     6241 ttgccagcgc cctcgctgat ggagtgattc tgggcagtag tcgttagtac gagaccattc
     6301 gcgaatttcc ccagtcggcg gccttcgatt gttcttgtac ctgagcccgt cgaaaattgc
     6361 tgggcatcac aggaacatcg gcgttatggt agtgcgtatg gaacgccttg tcgcaattct
     6421 cgcgcttttc ctgtccgctc cattcttgtt cgccgctgac ctttcgggaa tctggctgta
     6481 ttccgccaag cagcccgacg gccacaccgc gcgcatggtg cttgccctgc agcaagacgg
     6541 ggacaacctc gcgggcaaga tcgaacatcc ctggggcact ctcaacatca aggagggcaa
     6601 ggttgatggc aacagattca ccatcaccgc gcccatcagc gatggccgcc cttatatctg
     6661 cgaaggcacg ctggagggcg acaaacttca cttcacggtc cgcgatccca atgccaaacc
     6721 ctacgaagtc gtggccgtac gctcgccgtc cgatcccttc cgggtgacga cgccgctgac
     6781 tcctcccgcg gttcgcgatc tgcccggtaa cgggctggcc aaaactccgc ccatgggatg
     6841 gaacagttgg aacctgttcg ccggccgcat cgacgataag atcgtgcgcg agatggcgga
     6901 cgccatggtc tccagcggca tgcgcgatgc cggcttcatc tacctcaaca tcgatgacac
     6961 ctgggaaggc gaccgcgacg ccaacggagt gattcactcc aacaacaagt tccccgacat
     7021 gaaggcgctc gccgattacg tgcattccaa gggactgaag ttcggcatct actcgtcgcc
     7081 cggcccctac acctgcgcgg gatacgaagg cagctatgct cacgaggcgc aggacgcaaa
     7141 gacttacgcc gcctggggag tggactacct caagtacgac tggtgcagcg cgggcggact
     7201 gtatccggat tcggccatgc gtgccgttta tcagaaaatg gccgaggcgt tgcgcgccac
     7261 caatcgcccc atcgtttaca gcctctgcca atatggaaag gaaaatgtct gggagtgggg
     7321 tgcggaagcc ggcggcaact tgtggcgcac cactgacgac atcaacgaca cctacaaacg
     7381 catgatggaa atcgtggaca tccaatcggc catcgccaag tgggctggcc ccggacactg
     7441 gaacgatccg gacatgctgg aggtcggcaa cggcggcatg acccccgacg aataccgcac
     7501 ccacatgagc ttgtgggcga tgttcgcggc gccactgctg gcaggcaacg atgtacgcgc
     7561 catgtcggcc gacacgcgcg cgatcttgct taacaaagat gtcatcgcca tcgaccagga
     7621 cccgctcggc cagcaggggc aactcgcgct caagcgcagc gacgtcgact actggaagcg
     7681 acaactcagt ggcgggggcg tggcaatcgc ggcggttaac cggtcgagct ctccggtcgc
     7741 agtcaatatt ctgtggagag agttgaaagt cgacggcaac ctggttttca aggatgtgtg
     7801 gagcggtatg cagatcaaag gttcatccga ctccacatta accattcagg cgcacggcgt
     7861 ggtgctgttg cgctcggaac tggtgcattg agctaactaa tagttgcaca ctgtcgcaac
     7921 tatcctagcc gtgactcttc ccgattcctg gagcacctgg aaggcgcggg cgggaagccc
     7981 cgcgccttgt cgccgctagt ggccaccagc agccaccccg ttcaaccaca ttctaagcta
     8041 aggacgcgct attactttgc cagcttcttg aaccgaattg cgccaagcgc tcccatgaat
     8101 cctgtgccga gcaggagcaa gcagctaggc tcgggagtgc ccaggatgtc gaatgcatcg
     8161 agcgtacttc cgtagcccct gctgttccac atagccccat cgtcggtgct aaagagagtc
     8221 aggccggtct ggccttggct gttcaaattc cacgcgttca agcttgcatc gtcaagggcg
     8281 accggaccaa cgatcataaa atatgactga ccgccgatta gggtgactcc cgaaatacca
     8341 tggatagtga caaaccctcc gccatcgtag tcaccggggc atctaggagg gcaaaatcca
     8401 aattcggtga tcgtagtcag gggactccaa aaagcattgg gcacctgcga gcctggaaga
     8461 ccaccgttgt ctgtccagat gctggtatag aaggtgtgga gggattcaat gaatgtcaca
     8521 cccatatcaa tctgagttac gatttgaggg ccagagccgt ttacagtgaa tcggctggcg
     8581 tcaatatcgc tgaagcctgt gatgctgcca ctgccgctta cagtagctcc ctggcagcaa
     8641 ttgtattgat ctcctggagg tcccaaatcg gaaaatagcg tttgtgccat tgcgggcaaa
     8701 gcgctcacgc agagggcgag agcaagcatg aagatcaacc aaccaagttt cttattcact
     8761 tttgtacctc cctctgttgc tttcacctcc atctccattg cggcatccag aaatgccata
     8821 tcaggaatct cctttttgat tcccgcctcg gtctcagtgt attcctcgtg caatttccct
     8881 aacccaagat tgcatttccg aacaaagaat ggagtagtgc cgtaccattt tgggaattgc
     8941 cgccaatcga gatcgttcct tgcttaggtg atgtcatcac caacgaactg agggatctgc
     9001 tctctccacg aagagcaaat ccctcaaccg ttgcagcggt gtcgcactgt caaatccgaa
     9061 cggctacttc ttcacttcca ccggcgccgg tagtggcacg tcggaattga aaatgtcggc
     9121 caccaccttc catttgccgt cggcctgctt cttccaaact tcgacgaatt tgcctttgtc
     9181 agtcaccgga ttgccttgcg gatctttcat ggtcaactga taggtgccca tgatggaggc
     9241 caggtcgctg gaacgggcga cttcaacctt ggtcgcctgc caggagatgg acgttcccgg
     9301 accgagcaat ccggcccacg ccgcgtgaat cgcctgcttg ccggtcgcga tggggacgtt
     9361 gggagccagc agcgaggcgt catcggtgta gtacgcgacg gtgccctcca ggtcgttggc
     9421 tccggccgtc ttcgcccatt gcgcgtccgt gtctcgaatc gcggcttcat ccgctgcccg
     9481 cgtgtccggc ggcgcctggt tgcacgccgt tgagagcagc agtagagcga gacacccgca
     9541 caaagccagc ttgagcttct gtcgttccag aaaacgagtg gtcattggca attcctccta
     9601 taaagcaagc caagatatca cacccgcgcc ggcggctagc ccgcatccag cttggaacca
     9661 actcgtccga cactccaggt tggggaggtg taacgttggt gggtgttcgg gaagttggcg
     9721 cgcttgagcc tctgaaaatc tggcaggagt cccggtttcg agggacaatt gagacg
//
LOCUS       DASRKK010000830         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_877003, whole genome shotgun sequence.
ACCESSION   DASRKK010000830 DASRKK010000000
VERSION     DASRKK010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877003"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1290)
                     /locus_tag="VFC15_19855"
     CDS             complement(346..1290)
                     /locus_tag="VFC15_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12469.1"
                     /translation="MNYKTCTYVRDPGYLCQSAAVGGREYCCYHLRHRGRQMRMAKHR
                     ARGERFDLHLPPMENMHAVQSALSQVIEALAADMIDPKRAHELLLALRQAATNFRHPE
                     AWRASEYRNDQSAAYPASYEEFEAEYGLPEGIDLNTPPEVAFPPPSHPVGAPSSSPDF
                     GDRVGDIGCPIPPSFDRVPLIPEIPPPYVRDYTAEAEAAFESTPADIELTEIYNTQGY
                     KAWERRAQEHQRNADRKKQRKLFRANYARYAAEAKLKNIQAAAEKLLRERLAAEKAAT
                     QKEAAQTAQTEPVETKKPPARAVPEPAPATQKEVESIA"
     gene            2027..2266
                     /locus_tag="VFC15_19860"
     CDS             2027..2266
                     /locus_tag="VFC15_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12470.1"
                     /translation="MSDQEVFVQQFARWFQHFHEALSPDPEQESASWNLIPVDQRHRL
                     VAAARLAIHELETDARSQDNSRLYFAKPGEAEWGC"
     gene            2439..2759
                     /locus_tag="VFC15_19865"
     CDS             2439..2759
                     /locus_tag="VFC15_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12471.1"
                     /translation="MAVANTNSISQVASSAIRPALVEARLITDKHAADAVYMATLAEE
                     LRNIEAKIAAMEEANQFCLSVVATRSGPALVTEHNPDANWIRTKSRELVARLRRIEQL
                     SIKW"
     gene            3048..3323
                     /locus_tag="VFC15_19870"
     CDS             3048..3323
                     /locus_tag="VFC15_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12472.1"
                     /translation="MNMSTEELFVQQFAKLFAHYRDALTLDSGGEHGSELQGLNETQV
                     VELDRMVSAARLALLEIETGAREQDDARRYYAKAGGVESEPHGARGA"
     gene            3583..5088
                     /locus_tag="VFC15_19875"
     CDS             3583..5088
                     /locus_tag="VFC15_19875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM12473.1"
                     /translation="MHRQDPTKRASQWLAGRGIWQTALRAIPSSIVVGITTFACFTFH
                     VNFPTVSFIYLIIVVLQSLTGDFLSSAVVSVVAFLCLNFFFVPPIFSLAVSDSSDTLA
                     LISFLITGLVVTRLTSRARKAADSAALQRTETTRLYELARELLASDPNATVGIDLLKP
                     FKSQFSLGAVCLFDAVTAQLQLDGESLEHLAEKTRTAYISECNFQDRDSGVAVRLLRN
                     GDHFIGAIGFEGLHDVELTAEPLAALAAVMMERARAFHRASHAAAATEAEMFRGAVLD
                     ALAHEFKTPLATIITAASGLREAGPLRSEQKELAEIIESEASRLGQLTSRLLRLARLD
                     RDEVKPRMELTDLGKVVKSVVNQYVLRWPDRRLQLIKSDRVDVLADQELLRMGLGQLL
                     DNACKYSGTGSDIRVSIENANEAIAVRVWNNGTPIPAGEQARIFERFYRGVDARRQTP
                     GTGLGLYVARKIALAHGGNLGLEGPEGGGAGTAFRFTMPVSRNELGHDTEI"
     gene            5072..5779
                     /locus_tag="VFC15_19880"
     CDS             5072..5779
                     /locus_tag="VFC15_19880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM12474.1"
                     /translation="MTLRSNVLVVDDEPPLRRVLRTSLTARGFTVEEAGNAERALELV
                     RQRSFDLILLDMNMPGMGGIEACRRLRGLLPHVGIVMVTVRDAEEDMVQALNAGADDY
                     ITKPFRFGELVARLRAVLRRTVPENAGTTVIQAGDLEIDFDKRCLRRAGKEIHLTPTE
                     FDLLALLMRNPGALLPHAKLLRTVWGPEYGEELEYLRAYVRALRKKIEDDPAQPKYIL
                     TEPWVGYRFHNPSDDEE"
     gene            7231..>7696
                     /locus_tag="VFC15_19885"
     CDS             7231..>7696
                     /locus_tag="VFC15_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12475.1"
                     /translation="MKRIASSEPEKDQDVETMKTLSHKNIAIAICSLMLALGVPAKHC
                     YAQAQQGQAETATKVASLNEPGSDAAAGDVGGASVVTPSQTPAAVDAGSSAAIAKKLE
                     VMELRIQQLEAQLGERTAEEHPPVADLLSANPVKDAAPASAKAIGHFTVLPQT"
BASE COUNT         1665 a         2007 c         2256 g         1768 t
ORIGIN      
        1 ggtataccag tcggggatga atgaccgcca actcctacgg ttatctcgcg aaatttgccg
       61 aaattgcttt gcacgcttcc tttgttttca tcacgttagg ttgaaattgc tcgcatctca
      121 ttgattttat tgctagttat agctatccat gtctgcccgt tggacttagg tcgcctctcg
      181 tggcgtgtgc cgccggctaa agccggctcc ggataaagag gcgtcgtgac gccgcgctga
      241 agcgcggctc taccctaaac ctagctcgac taaggcgagc taatgacacg ccgacgcgca
      301 gcactaccat tgttctggct cggctacccc gattcgacaa atcatttagg cgatggattc
      361 gacctccttc tgggttgctg gcgcgggttc ggggacggcg cgagcgggtg gttttttggt
      421 ttcgaccggt tcggtctgcg cggtctgcgc ggcttctttt tgggtggcgg ctttctcggc
      481 cgccagtctt tctctgagca gtttctcggc ggcagcctgg atgttcttga gcttggcttc
      541 ggcggcgtag cgcgcgtaat tggcgcggaa gagcttgcgc tgcttcttgc gatcggcatt
      601 gcgctgatgt tcctgggcgc gacgctccca agctttgtag ccttgcgtgt tgtaaatttc
      661 ggtcaactcg atgtccgccg gcgtgctctc aaaagcggcc tcggcttcgg cggtgtaatc
      721 gcggacatag ggtggaggga tctcggggat aagcggaacc cggtcaaagg aaggcgggat
      781 gggacagccg atgtctccca ccctgtcgcc aaaatcgggc gacgaggatg gggcacccac
      841 gggatgggaa ggagggggga aggcgacttc gggtggtgtg ttgaggtcga tgccctcggg
      901 caggccgtat tcggcttcga actcctcgta ggaggcgggg taggcggcgg actgatcgtt
      961 cctgtactcg ctggcgcgcc aggcttcggg atgccggaag ttggtggcgg cctgacgcag
     1021 ggctaagagc aactcatggg cgcgcttggg atcgatcatg tcggcggcca gggcttcgat
     1081 gacctgcgaa agcgcggatt gcacggcgtg catgttctcc atgggcggca ggtgaagatc
     1141 gaagcgctcg ccgcgagcgc ggtgcttcgc catgcgcatc tggcgtccgc gatgacgcaa
     1201 gtggtaacag caatattcgc ggccgccaac ggcagccgac tggcataagt atccggggtc
     1261 acggacgtaa gtgcaggttt tgtaattcat gtagctcccc ctaagaagtg gctcgtggct
     1321 agtggccaga aaaaacagtt tcgagtttcg agttgccagt gaagccgccg acaagatggc
     1381 aaccgtccgg gcatggttct ccagttttta gggtagggag tggggagggg agagtctatg
     1441 aaaaaggggg cggcgatgtt acgaaggtgg ggggttgggt aacccctcaa ttggcaatcg
     1501 cggctattcc gggtgccgct catggcttcc gacaagggac gacagaacgg gtaggtcatg
     1561 caccgctggt cggttcccgt tgagggcata attgacggtc agcgtctgtg ctactctcgg
     1621 ctaccgatcg caccgagtac aattcttacg caatgttcct ccggaggcta gatggccgac
     1681 tataacttac acggactctc tacacgctca tttgagcaac tagtagggga tgacttcgcg
     1741 tctaccgaac cacggttacg atcaggtgtg taggttatgg atctcctgcg gggttgaagt
     1801 acgatccgcg agagtcaatc aaatgccgac tgagcccggc ccgggcatga tccacagaca
     1861 ctttcgttaa accagttgtg tttccacggc ggtactggaa caagttggaa ccgccgaaat
     1921 gaaaaccaat ccggtgcgga cttacaaact ctttacgcgc gatttattcg atccttacga
     1981 tcctctgtgt caaatctact gagcgtagaa acgcgccaga ggagatatgt cagatcagga
     2041 agtattcgtt cagcagtttg cccggtggtt ccaacatttt cacgaggctt tgtcgcccga
     2101 tcccgaacag gaaagtgcat catggaactt gattcctgtc gaccaacgcc accgtctagt
     2161 cgcggcggcg cgcttggcga ttcacgaatt ggaaactgac gctcgatcgc aggacaattc
     2221 ccggctgtac tttgctaagc ctggtgaggc ggagtgggga tgctgacgca acttttgctg
     2281 atagcacgag tttggatagg tccgtcgcag tcagtagtga tcagggagag gttggacgct
     2341 gtattaagtc tgttctcgga gctcaagcgc tttacgacgt ggcatatacg aaggtcatgt
     2401 ctgccgagcc gcaacaacta gatcgtctga ggtccaacat ggcggttgca aacacaaact
     2461 ccatttcaca ggttgcctca tctgccatcc gccctgctct agtggaagcc aggttaataa
     2521 cggacaagca tgcggctgac gccgtatata tggccaccct cgctgaagag ttgcgcaata
     2581 tcgaggcaaa gatcgccgcg atggaagaag ccaaccagtt ttgtctttct gttgttgcaa
     2641 cccggagtgg gcctgcgctt gtgacagaac acaatccaga cgccaactgg atacgcacca
     2701 agtcgcgcga attggttgct cgtcttcgcc ggatcgagca attgtccata aagtggtagg
     2761 tcggctgcat tgtcacgtgt gttgaatcac tcgagagccg gtctcaggga tggacctcgt
     2821 agtggactgc aacagtaagt actcagccag gttagtggac gaagctgcga ctcttaggaa
     2881 gttacagcag atgccgataa tcgaaaagtt tatgaagacc agtcaaacaa ttaatggagt
     2941 tcttcacgat tttttcacgc atagcggttt atctttcagt catcagaacc gacctcagcg
     3001 gaaaatcaga cccgcacgaa tcagcctacg ctaatagaag ggactggatg aacatgtcga
     3061 ccgaagagct ttttgtgcag cagtttgcca agctgttcgc acactaccgc gatgcactaa
     3121 cgctggacag cgggggagag catggaagtg agctacaggg gctgaacgag acacaagtcg
     3181 tcgaactcga tcgcatggtt tcagccgcgc gtctggccct gcttgagatt gaaaccggcg
     3241 ctcgggagca ggatgacgcg cgccgctact acgctaaagc aggcggagtg gaatcggagc
     3301 cccacggagc acgaggcgct tgatgcttgg gacgacggac gaagttatgg atagccgttg
     3361 tgcgaagagc cgcacctaga gcgctggctg ggtttgctaa gaggctggag gattggaccg
     3421 gcgttcattg tgcggcacgc acatgggtga cggtccccca tcgagggatc agggcgtagg
     3481 aggcccgtac actaagccct cgccagtcat gaagtatcag caaccgttaa gactgctctc
     3541 atacgacgtc atcagcgggc aaaccagccc gcgaactcga atatgcatcg acaagacccc
     3601 acgaaacggg cgtcgcagtg gctggctggg cgtggaatct ggcaaacagc actgcgggct
     3661 atcccgagca gtatcgtagt tggaatcacc acgtttgcct gctttacatt tcatgtcaac
     3721 tttccgacgg tgagctttat ttatttgatt attgtcgtac tgcagtctct aacaggtgat
     3781 ttcttgtcat ctgcggtcgt gtcggtcgtt gcttttctct gcttgaattt tttcttcgtt
     3841 cccccgatct tttcgctcgc agtgagtgat tcgtccgata ccctggcact gatctccttc
     3901 ttgatcactg gtcttgttgt tactcgattg acttcgcggg cacggaaagc agccgattca
     3961 gcggcgcttc agcggacaga gactactcga ctgtacgaat tggcgcgaga gttgcttgcc
     4021 tcggatccga acgcaaccgt aggcattgac ttgctaaaac cgtttaagtc gcagtttagc
     4081 ttgggcgcgg tgtgtttgtt tgacgccgtc accgcacaac ttcagctgga cggagagtct
     4141 ctcgagcatc tggcagaaaa gacgcggacc gcctatatct ctgaatgtaa cttccaagat
     4201 cgtgactccg gggttgcagt gcgtctgctg cggaatggcg accatttcat aggcgctatt
     4261 gggttcgaag gtttacacga tgtcgagtta accgcggagc ctttagccgc gctggcggcg
     4321 gtcatgatgg aacgtgctcg ggcgtttcac cgagccagcc atgcagcggc tgcgaccgag
     4381 gctgaaatgt ttcgcggagc agttctcgat gccttggcgc atgagttcaa gactccactc
     4441 gcgaccatta ttactgctgc aagtggcctc agggaagctg ggccgctgcg gtcggaacaa
     4501 aaagaactcg cggagattat cgaatcggaa gcctctcgtc tgggacaact cacctcgcgc
     4561 ctgcttcgct tggcgcgact cgatcgcgac gaggtcaaac ctcggatgga gttgaccgat
     4621 ctggggaagg ttgtgaagtc agtagtgaat caatacgtct tgcgatggcc cgaccggcgg
     4681 ctgcagttga taaaaagcga cagagttgac gtgctcgctg atcaggaact gctcaggatg
     4741 ggattaggtc aactgctgga caatgcctgc aagtactctg gcaccggttc cgacatccga
     4801 gtttccatcg agaacgcaaa tgaagcaatt gctgttcgcg tgtggaataa cggtaccccg
     4861 attcccgccg gcgagcaggc ccgtattttc gaacgctttt atcggggagt agatgcgcga
     4921 aggcagaccc ctggaacggg attgggcctg tatgtggccc gaaagatcgc gctggcacat
     4981 ggtggcaacc tcggtctaga agggccagag ggaggcggtg cgggcactgc tttccgcttt
     5041 acgatgcctg tttcaaggaa tgagttgggc catgacactg agatctaatg tattagtggt
     5101 cgatgacgag cctccgttgc gaagggtgtt acggacttct ctgacggcgc gcgggttcac
     5161 ggttgaggag gctggcaacg cagagcgcgc cctcgaactc gtccgacagc gttcattcga
     5221 cctgatcctg ctggacatga acatgccggg gatggggggc atcgaagcgt gccgtcgcct
     5281 gcgcggattg ttaccgcatg ttggaatcgt gatggtgacg gtgcgcgatg cagaagaaga
     5341 catggtgcag gctttgaatg cgggtgcgga cgactatatt acaaaaccct tccggtttgg
     5401 agaactagtc gcccgtctcc gtgctgtgct ccgccgtacg gtccccgaga acgcaggcac
     5461 gactgtgatt caagccggcg atctggaaat cgattttgac aagcgctgcc tgcgtcgcgc
     5521 gggcaaggag attcacctca cgcctacgga atttgatcta ttggcattgc tgatgagaaa
     5581 cccgggagcg ctactgcctc acgctaaact gctccgtacg gtgtggggtc cggagtatgg
     5641 cgaggagctc gaatacctga gagcgtacgt tagagcgctt cggaagaaga tcgaagacga
     5701 tcctgcgcaa cccaaataca tactgaccga accttgggtt ggataccgtt tccacaatcc
     5761 ttcggatgac gaggaatagg cgggaaatcc tgaagaccga agtcatacga gcttttgcaa
     5821 ggcggctgtc tgagagcagc cgctctcccc tcactttatc caaccgaaca tcgaaacggc
     5881 acaactgctc cctttcgatt ctggtaggta aactggaaac atccgcaccg aacatggcgg
     5941 agctgttcgg aagctttatg gcacggcggc caggctcacg ccaagtggtc tccgttctac
     6001 tcctcgggcc aacctatcca ctgataagtg ggacgtggct ggcccggtga acaactgagg
     6061 ccaagcgatt acgttttgta cgggacggcc tatcgcgcgc tagactcaag taccgctttt
     6121 tcttgattcg atttgcgcga tggctttgcg atcgacgata tcttgatcgc gtctccactt
     6181 agttcgccgc tcacgacgac atcgtccgct gcgaagtgtc ccagtcgctt agggtcgccg
     6241 gtcactgaat aggttttgtc ggttgtctgc aaggcgtact gggatccgtc ggggcccttc
     6301 gcacaatgga ggctacatcc ccagattgac tcattcttgc tgtggccgca agtatatgat
     6361 gctgccgaca tgttggcgca ggttagtcga cctctgaaag tctgctcgcc gactgccgaa
     6421 tcggactttg gcgctggcgc ggccccactc tgcaccttat gttcactctc caactggctg
     6481 atccgcgctt gcagcgtttg cacctgttgc tgtaactgtt gaacctccac ttcccaactc
     6541 gcctgttggc cggaggcctg cgcgaacagt ggaatgccgc aaacgaacaa cataagcccc
     6601 agcgccgtga acgtggcgca ccgtagtgcc catctccccc ttgaacaaac cggattcatt
     6661 gttatctcct ctcgattacc atggctcact ttgtcgttgt ccaagtctgg agtcgcggta
     6721 tcgatcaatt accatctgca cactattgct aaagagatga agaaggcata aagacttttt
     6781 cgctaatggc gacggaaata ctcaagcgga aggaactcca tcacagccgt gacatcgttg
     6841 ttgactttca cggcatatct cacttgttgt cgattattac cgtacggcct gtttggcgat
     6901 cggcaccgtc attctcagaa gagtatgaaa agaaaccgat aatcgaaaca tttatgacga
     6961 ctagtcaaac atttaatgga gatcttcacg gtttattcac gacctgcgcc atatcttctg
     7021 atcactaact tcgactctag cctaagacta gtcaccaact gaacaagagg agagacgtcg
     7081 cagaatgagg aagtggcgtt tacggatttc ttatatttcg tttgcttact ctttacgggc
     7141 cctttgattg aatgtggagg gttgcattga ctttcggcct caattaggaa ctacggctgc
     7201 ttgtttctcc ggtcgggcgt tcgtcaatca atgaagcgga tcgcgagtag cgaaccagaa
     7261 aaggatcagg acgtggaaac catgaagact ctgtcgcaca agaacatagc aatcgcaatt
     7321 tgttccttga tgcttgccct gggcgtccca gcgaaacact gttacgccca agcccaacag
     7381 ggacaggccg aaactgcgac gaaagtggca agcctgaatg aaccaggctc cgatgcggcc
     7441 gctggcgacg ttgggggagc cagtgtggtc acgccgagcc agacaccggc tgctgtcgat
     7501 gcagggtcat ccgccgccat cgccaaaaag ctggaggtca tggagttgcg tatccagcag
     7561 cttgaagcgc aactaggtga gcgcacggca gaagaacatc cgcctgtggc ggacctgttg
     7621 tcggctaacc ctgtcaaaga tgctgcacca gcgtccgcta aggcaatcgg tcactttacg
     7681 gtgttaccgc aaacgc
//
LOCUS       DASRKK010000831         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_486626, whole genome shotgun sequence.
ACCESSION   DASRKK010000831 DASRKK010000000
VERSION     DASRKK010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486626"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFC15_19890"
     CDS             complement(<1..333)
                     /locus_tag="VFC15_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12476.1"
                     /translation="MSSLAGYLADQHHISARTLWNWYGQYRKLGYAGLVDRVRSDKGK
                     SRFLKAHLAVRTFVENKYLGERLSIRLVYQALLRDLRTLEPECTRPPSYSAIRSYLQQ
                     LPKPLLILS"
     gene            complement(585..1646)
                     /locus_tag="VFC15_19895"
     CDS             complement(585..1646)
                     /locus_tag="VFC15_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZM12477.1"
                     /translation="MAAPDGFRLAEELRGTFLSYLTSALPIGNHQSQRELGKHFYQQW
                     SSELFAGPLVEALPVYQKVGSLDDYFGDARDKQSDDFKFAASMNVSLDWVDVDHRFPQ
                     YRKIRDAIWPPASDDAEEEAVKTSANRFWERSLYEHQWQSFEKVARQHRNLIVATATG
                     SGKTECYLISLLHHLLTESAEKRRQPGIRALLLYPMNALVEDQMHRLRQLLFWINLQN
                     YASGKARRPITFGRYTGATPVASNDRNPERQVSPEALHDLGELIYRSEMQTQPPDILV
                     TNFTMLEYILLRDDDQGKTWAPNCGSIRKLSEAQPDGPTERSVGRCSSERYGCGKPAT
                     GKNEASANTHCLPYRSKRS"
BASE COUNT          466 a          654 c          647 g          539 t
ORIGIN      
        1 actcaggatc agcaagggct tgggcagctg ttgcaaataa gaccggatgg cgctgtagct
       61 cggcggtcgg gtgcactctg gctccagtgt gcgcagatcg cgcagcagtg cctggtacac
      121 caggcgaatc gacagccgct cacccaggta cttgttctcc acaaatgtgc ggaccgcgag
      181 atgggctttg agaaaccgcg actttccctt atccgagcga actcggtcga ccagacccgc
      241 atagccgagc ttgcggtact gtccgtacca gttccagaga gttcgagcgc tgatgtggtg
      301 ctgatccgca aggtagccgg ccagtgagct catgcttcgc acggcaactc cgccgatggt
      361 acgaaacgtc ggacgggatc gctggctgcg cctcgagaat tccacgagtg gcgcaatggc
      421 cttcaggcgc tttaagacct gtgcgttctg cctagcggaa cagttgaggc gctcgggttc
      481 ggtgacctcc ggcagagaag caaacaggtt ggattggttt ggatccgagc gaagcgccag
      541 agccgtgcag gctggtcctg acagcagcgg ctgcttgagg aatttcagct gcgcttcgac
      601 cggtaaggaa gacagtgcgt attcgcgctg gcctcgtttt tgccggtcgc tggctttccg
      661 caaccgtaac gctccgagct gcaccttcca acggatcgtt cggtcggacc atccggttgc
      721 gcctctgaca gcttccttat tgatccacaa ttcggcgccc acgtttttcc ctggtcgtca
      781 tcgcgcagca aaatgtattc gagcatcgtg aagttggtta ccaaaatgtc aggtggttgc
      841 gtctgcattt cggagcgata aataagttcg ccgaggtcat gcaatgcttc cggagaaact
      901 tgccgctccg ggttcctgtc attgcttgcg accggtgttg caccggtata acgcccaaac
      961 gtgatcgggc gacgcgcttt gcctgatgca taattctgaa ggtttatcca gaacagcagc
     1021 tgccgaagtc gatgcatttg gtcttcgacc aatgcgttca tcggatatag gagtaatgcc
     1081 cgaataccag gttgtcggcg tttctcggcg ctttcggtga gtaagtgatg aagaagagag
     1141 atgagatagc attcggtctt gccactacca gtcgcggtcg cgacaattag attgcggtgc
     1201 tgtcgtgcca ccttttcgaa cgattgccat tgatgctcgt acagagacct ctcccagaat
     1261 cgattagccg acgtcttgac ggcctcttct tccgcatcgt ccgaggctgg aggccagatt
     1321 gcatcgcgaa tcttgcgata ttgcgggaat cgatggtcga cgtccaccca atccagcgat
     1381 acgttcatac tggccgcaaa tttgaagtca tcgctctgct tgtcgcgggc gtcaccgaag
     1441 taatcgtcca gcgagccgac cttttggtaa accggcagcg cttcgacaag gggaccggcg
     1501 aacagttctg aactccactg ttggtagaag tgctttccca gttcgcgctg tgattggtgg
     1561 ttgccgatgg gcaacgcact cgtcaagtaa ctgaggaatg ttccgcgcag ttcttccgca
     1621 agcctgaatc cgtccggagc agccatggcc gatgaatgtc ctccttcttg aatccctgcc
     1681 ttggttcttg gatagatctg gtactccgac tagacacgct gaccaacaaa aacctccaac
     1741 aagccttccc gtacttgaca gtggtcttaa ctgaaactat cggtatccgg aggacacggt
     1801 ccgcgagaga caattaccaa acgttccttg tgggagcgga agcgactcgt cgaacgcaag
     1861 ggcttccttg gccagaagct acagattaga tatctccatg caggttgtca gtgagcctta
     1921 tcacaccgtt cgcagcgcgc taaagtgcac cattgtcgca gcagaattgc cattccagcc
     1981 ccaaaccgcg tcaatttggc gtctggtagg cgcgtaaaag gcctcacgtt tgcgctacgt
     2041 ggcgctacgt cgcacccgtg gccccgggta ggcatttacc ccccataacc ccttgttttt
     2101 gagcgtcgta gcgcaaattt gggcttaagt ccgctcttcg tttcggcctg ggctcaacag
     2161 acgccaccaa aatcacttgg tcgcgtcgcc cggtttcgcg gcgtcggctg gcttcgtgtt
     2221 caccttggtc agcgattctt ccacccgctt cttgaagtcg ccggcgtcgt cgtgcgcaag
     2281 gccgctgtag ctcgacacca cttgca
//
LOCUS       DASRKK010000832         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_877521, whole genome shotgun sequence.
ACCESSION   DASRKK010000832 DASRKK010000000
VERSION     DASRKK010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877521"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2163
                     /locus_tag="VFC15_19900"
     CDS             <1..>2163
                     /locus_tag="VFC15_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12478.1"
                     /translation="VQVRLALAGLTQTVTVRGQPEAVETQGSDVSQVIAQQAIQGLPL
                     NGRRFTDLALLTPGVTQDPRGLTSDSNGDLSFGGVRGFQNNFLVDGTDNINSFYAQAR
                     GRYRAPYQFSNEVIKEFRVSSNSYSAELGRAGGAVFNVATKSGSNQFHGSGFYYLRDR
                     DFDAQQPYATTKPNDRQQQFGGSIGGPIKKDRVFFYAGFDQHLLTVPSIVQFANGASS
                     VVPQPQDYDYTDRQLVFSAAQTLNDMGGVFPTTMNGYAAFEKVDFTLSPKELVFVRLN
                     TSRYRGTNNVFFDPSSPMTSYAESGNGSESVQTESLAASLTSAWTNNLANNLRAQFSR
                     DVQQSTANSEQPLTKIYDVIYGFGRSSMLPRDTREHKLHLADTLSYETRRVHWKFGGD
                     FIQAWVYNYYPSMSGGEYYFDDVKVDPWTYAPMKHGEPLTPLRAFAHDVPRYYMQDFG
                     TAVSHPNSRLYSAFMQDTIRVSRSFTLNLGVRYDLQTFQAGNLIANPLYAPSGKIPTD
                     VNNVSPRIGFAYGIGDRHPLMVRGGFGIFYTQIPSMYVSQVATDNGISQSSIFLDNMI
                     PAQAAVFPKYPNPLVNCPTGTLVCMPPASLAGLLNTQVSAFAPNFQTPYVEQANLTLE
                     RDLGRKIVASVNYQYVHGVHLLRSLDANLPKPTITDYPVYSDDGSVFLNSYYQVASFA
                     TWQTTRSVTCPYPPCINDVQRPDPRLGTINSYESGSSSV"
BASE COUNT          466 a          698 c          554 g          445 t
ORIGIN      
        1 gtgcaggtac ggctggcgct cgccggactc acgcaaacgg tcacggtgcg cggacagccg
       61 gaggcggtgg agacgcaggg cagcgatgtt tcgcaagtca tcgcccagca agccatccag
      121 gggttgcctc tgaacggaag acgcttcacc gacctggcgc tcctcactcc cggagtgacc
      181 caggacccgc gcggtctcac ctctgactcc aatggcgact tgtcgttcgg cggagttcgc
      241 gggttccaga acaacttcct ggtggatggc acggacaaca tcaactcttt ctacgcgcag
      301 gcgcgaggcc gctatcgcgc cccctaccag ttcagcaacg aagtaatcaa ggaatttcgg
      361 gtgtcgtcca attcgtacag cgccgaactg ggaagggccg ggggcgcggt gttcaacgtg
      421 gcgaccaagt cggggagcaa ccaatttcac ggcagcggct tctactacct gcgcgatcgc
      481 gacttcgatg cccaacagcc gtatgccacc actaaaccta acgatcgcca gcagcagttt
      541 ggcggttcca tcggcggccc gatcaagaaa gaccgagtgt tcttttatgc cggatttgac
      601 caacacctgc tcaccgttcc ctcgattgtg caatttgcca acggcgccag cagcgtagtt
      661 ccgcagccgc aggattacga ctataccgac cggcaactgg tcttcagcgc ggcccagacg
      721 ctaaacgaca tgggcggagt attccccact acgatgaacg gctatgccgc gttcgagaaa
      781 gtggatttca cgctctctcc aaaagagctc gtgttcgtaa ggctaaacac ctcgcgttac
      841 cggggaacca acaacgtttt cttcgatcca tccagtccga tgaccagcta cgcagaaagc
      901 ggcaacggat cggagagcgt gcaaaccgag agcctggctg catcgttgac cagcgcctgg
      961 accaacaacc tcgctaataa tctacgcgcg cagttctccc gcgatgtgca acagtccacc
     1021 gccaactcgg aacagcctct taccaaaatc tatgacgtga tctacggatt tggacgatcc
     1081 agcatgcttc cccgtgacac ccgcgaacac aagctgcacc ttgccgacac cctgagttac
     1141 gaaacccggc gcgtgcattg gaagttcggc ggcgatttta ttcaggcgtg ggtttacaac
     1201 tattaccctt ccatgtctgg gggagagtat tacttcgacg acgtcaaggt ggatccctgg
     1261 acgtacgctc cgatgaagca tggagagccc ctgacgccgc ttcgcgcctt tgcgcacgac
     1321 gtacctcgct actacatgca agactttggc actgccgtct cccatcccaa ttcgcgtctt
     1381 tactccgcct tcatgcaaga caccatccgg gtctcccgga gctttacact gaacctcggg
     1441 gttcgctacg atttgcagac atttcaggct ggcaatctga tcgccaaccc cttgtacgcg
     1501 ccatcgggca agattcccac cgacgtgaac aacgtgtctc ccaggatcgg cttcgcgtac
     1561 ggcatcggcg accgccatcc cctgatggtt cggggaggct tcggcatctt ctacacccag
     1621 attccgtcca tgtatgtctc gcaagttgcc actgacaacg gaatttcgca gagcagcata
     1681 tttctggata acatgatccc ggcgcaagct gcggtgtttc cgaaatatcc caatccgctg
     1741 gtcaattgcc caaccggaac cctggtatgt atgccaccag cctcactcgc cggcttactc
     1801 aacacccaag tttcggcctt cgcacccaat ttccagacac cgtacgtgga gcaggcaaac
     1861 ctgactctgg agcgcgacct tggccgcaag atcgttgcct cggtcaacta tcagtacgtg
     1921 catggtgtcc atcttctgcg ctctctggac gccaatctgc caaagcccac gattaccgat
     1981 tacccggttt acagcgacga tggctccgtc ttcctcaatt cttattacca ggttgcgtcc
     2041 ttcgccacct ggcaaaccac ccgctcggtg acctgcccct atcctccctg catcaacgac
     2101 gtccagaggc cggacccgcg gctgggaacc attaactcct acgaaagcgg atcgagcagc
     2161 gtt
//
LOCUS       DASRKK010000833         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_389907, whole genome shotgun sequence.
ACCESSION   DASRKK010000833 DASRKK010000000
VERSION     DASRKK010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389907"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFC15_19905"
     CDS             <1..637
                     /locus_tag="VFC15_19905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM12479.1"
                     /translation="RATLYESAYKIAKEESAARSLADSLYAALYGVDAKGQQRTSKLR
                     SYQGRGSLQGWLRTVVAQEYVNLYRATRRETSLDAALDEGIQFEAGEPDLSVADPRAE
                     AAAAEELTALAAEERFLLAAYYLDRRTLAEIAKLQGVHESTISRKLERVTSGLRKRIR
                     KRMIEAGMSPRQADEAMQDVDVRDLRVRVRETLRQGTGDIPFYKEKSGNQG"
     gene            634..1806
                     /locus_tag="VFC15_19910"
     CDS             634..1806
                     /locus_tag="VFC15_19910"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZM12480.1"
                     /translation="MNQEIPKPLRNALARQAVGEVHPSPDVLTSFLECTLPPVESEVV
                     THHLAQCAECREIVFLASDAAEDEVRVERELVAAAAASRVAPMPVYTATSRPAAAWAE
                     PSRPRWTSRMRWALSVAAAALLVSGGLVLQFSRAGSGHSAVPLTVASNRPALASPEAQ
                     QNATAQESQEAAATPRAPEALARAAPHRAMAAPAKKGPAQTTGARNAADQFPPAPAVQ
                     PSPQTTPSQATAGAVGDMNSAMVPEVRPQSTFAESEAAQAVQRAAPLAFGKTQAGMHS
                     VSAARPQWRIGPEGHLERSMAAGQWTRVLDEQPTTFHAVAVMGNDVWAGGNGGALFHS
                     SDGGEHWSEVSLAANSNVETGAIVSIRFDDPQHGVVACDSGTRWATADGGVTWMTH"
     gene            1845..>2611
                     /locus_tag="VFC15_19915"
     CDS             1845..>2611
                     /locus_tag="VFC15_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12481.1"
                     /translation="MKNSDLARQPVRLSSMGTPVMVLVTMAAAPYLFQACIAIATGRH
                     LFGDGSWLLLRLLAENHITHLNNKTWNDFFVGRIGSFSYQQLPTLLASRMGVHNLKIL
                     CVVFGITLFSFKPLSILLCYRFARDKRYVLFPLLSLFAGTMNSEGYLISETHLMSALF
                     WAALFGLLYPREFGWFDLAAMTVVSAPLLLCYETMAVFGLVLVGACIYRLHAIAKSPQ
                     EKWLTMVFGTWYGLGVVFAILAIIFPRDPTQRADFFKG"
BASE COUNT          490 a          747 c          844 g          530 t
ORIGIN      
        1 tcgcgctacg ttgtacgagt cggcatacaa gattgccaag gaagagagtg ccgcgcgcag
       61 cttggcggac tcgctgtatg cggcgctcta tggtgtggac gccaagggac agcagcggac
      121 ttccaaactc cgttcctatc agggacgagg ttctctgcag ggatggctac ggactgtagt
      181 cgcgcaggaa tatgtcaacc tgtaccgggc tacccggcgc gagaccagtc tggacgctgc
      241 gttggacgag ggcatccaat ttgaagccgg ggagccagat cttagcgtcg ccgatcctcg
      301 tgctgaggcc gcggctgcag aagaactcac ggcgctcgcc gcggaagagc gtttcctgct
      361 ggccgcctac tatcttgatc gccgaacgct ggcggaaatc gccaaactgc aaggggtgca
      421 cgagtccacc atcagccgca agctggaacg cgtgacctct ggtttgcgca agcggattcg
      481 caaacggatg attgaggcgg gaatgtctcc gcgacaagcg gacgaagcca tgcaggatgt
      541 ggatgtccgc gacctgcgcg tgcgcgtgcg cgaaacttta aggcaaggaa cgggagatat
      601 accgttctat aaagagaaga gcgggaacca aggatgaacc aggaaattcc caagccgctg
      661 cggaacgcgc tggccaggca agcggttggc gaagtgcatc cttcgcccga cgtgctcacg
      721 tcgttcctgg agtgcacgtt acctccggtg gagagcgaag tggtaacgca ccatttggcg
      781 cagtgtgcgg aatgccgcga gatcgtgttc ctggcgagcg atgcggcgga agatgaggtg
      841 cgtgtcgaaa gggaattggt ggcggcagcg gccgccagtc gggtggctcc catgccggtg
      901 tacaccgcta cctcacgtcc ggctgctgct tgggctgagc cgtctcgccc cagatggaca
      961 agtcggatgc ggtgggcatt gtcggttgcg gctgccgcgt tgctcgtgtc tggaggactg
     1021 gttctgcagt tctctcgcgc cggcagcggg cacagtgcgg tgccgctcac cgttgccagc
     1081 aatcgcccgg cgctggccag tcccgaggcg cagcaaaacg cgaccgctca agagtcgcag
     1141 gaagctgccg cgacgcctcg tgcgcccgag gcactcgcaa gagctgcgcc gcaccgggcg
     1201 atggctgcgc cagctaagaa gggtcctgca caaactaccg gcgcccgcaa tgccgccgac
     1261 cagttcccgc ccgcgccagc ggtgcagcct tctccgcaaa cgacgccttc acaagcgact
     1321 gccggcgctg ttggtgacat gaacagtgct atggttcctg aggttcgtcc ccagagcacg
     1381 ttcgcggaga gtgaagccgc gcaggcggtg cagcgggccg ctccattggc gttcggcaaa
     1441 acgcaggcgg gaatgcattc ggtgagcgcg gccagaccgc aatggcgcat cggccccgag
     1501 ggccaccttg agcgctcgat ggctgcgggc caatggactc gcgtgctcga cgagcaacct
     1561 acaacgtttc atgcggtggc ggtcatgggg aacgacgttt gggctggtgg caatggcggt
     1621 gcgctcttcc actcgtccga tggcggcgaa cattggagcg aagtttcact ggccgcaaat
     1681 tccaacgtcg agactggcgc catcgtttcc attcgcttcg atgatccgca gcacggagtc
     1741 gtcgcttgcg acagcggaac tcgctgggcg acggccgatg gtggagtcac ctggatgacg
     1801 cactgattgc ggtgcagcga agtccgcttc cgatttacag cacaatgaag aacagcgatc
     1861 tggcgcggca gcccgtaagg ttgagttcga tggggacgcc ggtgatggtg ctggtgacaa
     1921 tggcggctgc tccgtatctg tttcaggcgt gcatagcgat tgcgactggc cggcacctgt
     1981 tcggggatgg ctcgtggctg ttgcttagac tgctcgccga aaaccacatc acgcatttaa
     2041 acaacaagac atggaacgat tttttcgttg gacggatagg gtccttcagc tatcagcaac
     2101 ttcccacgct actcgcctcg cgcatgggtg tgcataactt gaagattctg tgcgtcgtct
     2161 tcggtataac gctgttttcg ttcaagccac tcagcatcct gctctgttac cgatttgccc
     2221 gcgacaaacg atatgtgctt ttcccactgc tttcgctctt cgccgggacg atgaacagtg
     2281 aaggctatct gatttccgag acccatctca tgagcgctct gttctgggca gcgctgtttg
     2341 ggctgttata tccgcgcgaa ttcgggtggt tcgatcttgc cgcaatgacg gttgtgtcgg
     2401 ccccgctcct tctttgctac gaaaccatgg cggtgttcgg actggtgctg gtcggggcct
     2461 gcatttaccg gttgcatgcg atcgcgaaaa gcccgcagga gaaatggctg acgatggtct
     2521 tcggtacgtg gtacggcctg ggggtcgtct tcgccattct ggcgatcatc tttcctcgcg
     2581 atcctacgca gcgcgcagat tttttcaagg g
//
LOCUS       DASRKK010000834         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_97430, whole genome shotgun sequence.
ACCESSION   DASRKK010000834 DASRKK010000000
VERSION     DASRKK010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97430"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFC15_19920"
     CDS             <1..414
                     /locus_tag="VFC15_19920"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM12482.1"
                     /translation="YRRTRDAGFGAEVKRRIMLGTYVLSAGYYDAYYLKAQRVRRLLA
                     SDFDQAFTKVDAIVTPTAPTPAFKLGEKMNDPLAMYLADIFTVTANLAGIPGISIPCG
                     KSKEGLPIGMQILGKHFDEAMVLRVGHAVEHAMAG"
     gene            complement(455..670)
                     /locus_tag="VFC15_19925"
     CDS             complement(455..670)
                     /locus_tag="VFC15_19925"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZM12483.1"
                     /translation="MSRWPSTKAGKVLKALGRIGWMIKGYKASSHVQLVHPKLGEYTW
                     AFDDSDELGPAMLAKISKKTGLKREDL"
     gene            complement(667..897)
                     /locus_tag="VFC15_19930"
     CDS             complement(667..897)
                     /locus_tag="VFC15_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12484.1"
                     /translation="MPAVAPKRRLKLLTIELDREEDGRWIADVPALPGVVVYGHAKQD
                     ALAKVQALALRVLASKIEEDGTLQDEVRFVLA"
     gene            1095..1379
                     /locus_tag="VFC15_19935"
     CDS             1095..1379
                     /locus_tag="VFC15_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12485.1"
                     /translation="MADHVIHISEAEAASDFASVLARVRAGAEVVIESEGGKLPVAVI
                     HAPVAPRRTISECIALLPEDSTAVMDADFAKDVEAAIESHREPLEPPPWD"
     gene            1370..1684
                     /locus_tag="VFC15_19940"
     CDS             1370..1684
                     /locus_tag="VFC15_19940"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZM12486.1"
                     /translation="MGLILDSSVLITAERQGQNARQMLAAISGSPAGNTEIAISVVTP
                     IELAHGAARADTPQRRAKRHQFIQELLTALPIHPITVQYAPARSTARIRRRASAFLSP
                     IC"
     gene            complement(1876..2460)
                     /locus_tag="VFC15_19945"
     CDS             complement(1876..2460)
                     /locus_tag="VFC15_19945"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZM12487.1"
                     /translation="MKTLDELLRDAEEAHGHLCAGQVLGVRLAMLGLELLGIDDPRGK
                     DRKRLITFVEIDRCATDAVAVVTGCRLGKRALKFRDWGKVAATFVDLASERAVRVCAR
                     ESSKMLARQMHPEIENKNQQQMLAYREISNDDLFTVQWVKVALPPEELPGYKGERIVC
                     EQCGEGINFQREVRREGKTLCRACAGESYYEEIS"
     gene            complement(2480..>2729)
                     /locus_tag="VFC15_19950"
     CDS             complement(2480..>2729)
                     /locus_tag="VFC15_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12488.1"
                     /translation="GTTTNGEKLDAVVVQIGVKKPLYRKELGKANGGEQPDSDCSAPH
                     WDRQPARVTFLPKVGDKLSVTVRGRFVDEVPDDDSDEP"
BASE COUNT          543 a          843 c          772 g          571 t
ORIGIN      
        1 taccggcgca cgcgcgacgc gggttttgga gcggaagtca aacggcgcat catgcttggc
       61 acttatgttc ttagtgccgg atactacgat gcctattacc tgaaggcgca acgtgtgcgc
      121 cgtttgctgg cgagcgactt cgaccaggca ttcaccaagg tagatgcgat tgtcacaccc
      181 accgcgccga cgcccgcgtt caaactcggc gagaagatga acgatccact ggccatgtat
      241 ctggcggaca tcttcacggt gacggcgaac ctggccggca ttccggggat ttctattccg
      301 tgcggcaaga gcaaagaggg tttgccgatc ggaatgcaga tactggggaa gcacttcgac
      361 gaggccatgg tgttgcgtgt gggtcatgcg gtggaacacg cgatggcggg gtagagcagc
      421 cgagcgatcg ccgtgttcaa ctgaatacgg cagattaaag gtcttcgcgc tttaaccctg
      481 tctttttgga gattttggct agcatcgcgg ggcccaactc gtctgaatcg tcgaaggccc
      541 acgtgtattc gcctagcttg gggtgaacca attgcacatg ggaagacgct ttgtacccct
      601 tgatcatcca accaattcgg ccaagggctt tcaggacttt cccagccttg gtgcttggcc
      661 agcgactcac gctaagacga accgcacttc atcctgtagc gttccatcct cttcaatctt
      721 gctagccaag acccttaacg ccaacgcctg caccttagca agtgcatctt gcttcgcgtg
      781 tccatagacc acgacacccg gaagagccgg aacatcggct atccagcgtc catcttcctc
      841 ccggtcgagt tcgatggtaa gcaacttaag gcgtcgcttc ggcgcaacgg ctggcatcaa
      901 attcctcggt gtgaaaactg attctctgac actacgagtg tacctcgcct gtcaatcata
      961 tgggcgtaca cctgttggag cggaacacca gggcatgggt tgtgtaatgg cattggcaag
     1021 aaagggcttg atttcagttg gttgatgttg attttccagg ctgggagggt tgcataaacc
     1081 ggtataatcg cgccatggcc gaccatgtca tccacatctc ggaagcggaa gccgccagcg
     1141 acttcgcttc ggtgctggcc cgcgtgcgtg cgggggcgga agtcgtcatc gagagcgagg
     1201 gcggcaagct gcccgtggcg gtgatccatg cgccggtggc gccccgccgc accatctccg
     1261 agtgcatcgc gctgttgccc gaagactcga cagcggtcat ggatgcagac ttcgccaagg
     1321 atgtcgaggc agctatcgag agccaccgcg aaccgctaga accaccgcca tgggactgat
     1381 cctcgactcc agcgtactga ttacagccga gcgccaagga cagaatgcgc gacagatgct
     1441 cgcggccatc tccggcagcc cagcgggcaa taccgagatc gcaatctccg tggtcacgcc
     1501 aattgaactg gcgcacggtg cggcccgcgc tgacacaccg cagcgaagag ctaagcggca
     1561 ccagtttatc caggaactgc tcacggccct gcccatccat cccatcaccg tgcagtacgc
     1621 accggccaga tcgacggcga gaatcaggcg aagggcgtcc gccttcctct ctccgatctg
     1681 ctgatcgggg tgaccgcctt ggaactcggc tacagcgtag cgactgccaa ccttcgccac
     1741 ttccagatga tccccggcct caagatcgtc tccctgtagg ccgcgttctc gtggcgacgg
     1801 accccgttgg ctgccggcaa tcccaagctt gttgcggcgt caggcggcca cgccgcaatc
     1861 ccgcgccaac tttgcctacg aaatctcttc gtaatagctc tcgccggcgc aagcacggca
     1921 tagggtcttt ccctcacgcc gtacttcgcg ttggaagttg atgccttcgc cacattgctc
     1981 gcacacgatc cgctcgccct tgtagccggg caattcttcg ggcggcagag caacctttac
     2041 ccactgcaca gtgaacaggt cgtcgttcga tatttcgcga taagccagca tctgctgctg
     2101 gttcttgttc tcgatttcag gatgcatttg ccgggccaac attttcgagc tttcccgggc
     2161 acagacccgc acagcccgct ccgaggcgag atcgacaaag gtcgccgcca ctttgcccca
     2221 gtcacggaac ttgagtgccc gcttgcccaa ccggcagccg gtcacgacgg caaccgcgtc
     2281 ggtggcacag cgatcaatct ctacgaatgt gatgagacgt ttgcgatcct tcccgcgagg
     2341 gtcatcgatg cccaatagtt ccagtccgag catggccagc cgaacgccca gcacctgccc
     2401 ggcacagaga tgtccgtggg cctcttccgc gtcgcgcagc agttcgtcaa gggtcttcaa
     2461 tggaaacttg cactccgtct tatggctcgt ctgaatcgtc gtcgggcact tcgtccacaa
     2521 agcggcccct gacggtcacg ctgagcttgt caccaacttt gggaagaaag gtcacgcgcg
     2581 caggttgacg gtcccagtgc ggggcggaac aatcggagtc gggctgttct ccgccattcg
     2641 ccttccccag ttctttgcga tacagcggct tcttgacgcc aatttgcacg acaacggcat
     2701 cgagcttttc accattggtg gttgtgccg
//
LOCUS       DASRKK010000835         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1171744, whole genome shotgun sequence.
ACCESSION   DASRKK010000835 DASRKK010000000
VERSION     DASRKK010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171744"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(666..1418)
                     /locus_tag="VFC15_19955"
     CDS             complement(666..1418)
                     /locus_tag="VFC15_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM12489.1"
                     /translation="METQVQERTTVERQMFTARLSRSVLLSPQTKHLEFTVEGLEEFH
                     FVPGQFVSIKQPRPDGKTHTRAYSIASPPRLNATFDLCLNRVEEGFLSNWLCDLPEGA
                     TVQFHGPHGLFTLREPRKDAVFIATGTGIAPIRGMVHWLFEKPERNRGHEYWLVFGTR
                     YEHSLYYRDEFERIERENPNFHYVPTLSRCGELWTGCRGYVQDHVREIVAGRTDMQAY
                     ICGLHQMVDASRKLLKEELGWDRKQIVFERYD"
     gene            complement(1823..>2109)
                     /locus_tag="VFC15_19960"
     CDS             complement(1823..>2109)
                     /locus_tag="VFC15_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12490.1"
                     /translation="VHGAATPEQALRYAMSLPVSSTISGVDSMDVLEQNLAVARGFQP
                     MPAQEMQALREQCKFFASDGRYELYKVTKKYDGEVGREQHGFPTSGELPA"
BASE COUNT          457 a          610 c          607 g          435 t
ORIGIN      
        1 tttcgcccac ttcgcctcgt cggccttgcg cacctgtttc cagtcctgcg cgagcgatcc
       61 cgacgaaatc agaagaatgc aagcagcgac gagagcgact acaggggagg ctgtaaaagg
      121 cgacgttcgg gtagtgcggg ccttcaggcc cgcgtacaag ctattcataa tagtcaaggg
      181 gctttagccc ctgaggtaac tgaaatgact ttcttaacat cagcccctac agcaagacgg
      241 accatagctc atcttcatca tggcgcagcc aggttgtgag ggcaagattt tagtttagag
      301 ggattccaga gtggctgcgg ctcgggtagt gcgggccttc atctggatta ttcatctgtt
      361 ttggtagctt tgaaggggca cggcttcagc cgtgccgcgt gtgcgtgcat agtgtggctt
      421 tagcccctga gggaactcgc actgcgcccc aacaccgaag tccctcagtg gctaaagcca
      481 ccatgatgac agcaggttac ggcacggctg aagccgtgcc ccttcaaagc ctaaaatcct
      541 cgcgaacaat ccaggttcag acccgcgtta agagcgccca cgcaattctc ggctgaagcg
      601 atttttcttc tcgcccttga cctgaaagcc ccgcacgagc ggataacgcc aatcctgaat
      661 ccgccctagt cgtagcgctc gaacacgatc tgcttgcggt cccagcccaa ctcttctttg
      721 agcagcttgc gactcgcgtc taccatctga tgcagtccgc aaatgtacgc ctgcatgtcg
      781 gtgcggccgg cgacgatctc gcgcacgtgg tcctgcacgt agccgcggca gcccgtccaa
      841 agctcgccgc agcggctgag cgtcggcacg tagtggaagt tcgggttctc ccgttcgata
      901 cgctcgaact cgtcgcggta gtagaggctg tgctcgtaac gggttccgaa gaccagccag
      961 tattcgtggc cgcgattacg ttccggcttc tcgaagagcc aatgcaccat gccgcggatg
     1021 ggcgcgatgc ccgtcccagt ggcgatgaag actgcgtctt tgcgcggctc gcgcaaggta
     1081 aaaaggccgt gcgggccatg gaactggacg gtcgcacctt ccggcagatc gcaaagccag
     1141 tttgaaagaa agccctcctc cacgcggttg aggcacagat cgaaggtggc attcaagcgt
     1201 ggcggcgaag cgatggagta cgcgcgggtg tgcgtcttgc catcaggccg gggctgcttg
     1261 atggaaacaa actgcccggg aacgaagtgg aattcctcca gtccttcaac cgtaaactcc
     1321 aagtgcttgg tctgcggcga cagcaaaaca gaccggctca agcgagctgt gaacatctgg
     1381 cgctcgacgg ttgtgcgttc ctggacctgg gtctccataa tggtctgttt atgtagatgc
     1441 ggtcagccgg aaatcgtcgc gtgaagccgc gatctcaacg gttgggtaca ggcgccacac
     1501 aaaacggaaa tcttgagatg gaaatatccc accctgtcgc tgcgcgacaa gggaggggca
     1561 cccggcaaga ataacgaact taaagaagga gaacatcaat gcttattggg tctcttcagc
     1621 aacgagtctg gcacagtgca gactcaagga gcgtaagcgg tactcttttt gtaactttaa
     1681 ggcattagca ttagtgcatt tcgacgatgt gcttcaaggc tcctcttctg caggcgttcg
     1741 aaacagtggc gaaccacaga agcgcggaag attgcccgcc tcgttcatcg cgggccgaac
     1801 gagaaggtca gcaaaagcac gcctacgccg gcaactctcc tgacgtcggg aaaccgtgct
     1861 gctcacggcc gacctcgcca tcgtacttct tggtcacctt gtatagttca tagcgcccgt
     1921 cggaggcaaa gaacttgcac tgttcccgca gcgcctgcat ctcttgcgct ggcatcggct
     1981 ggaatccacg ggccaccgcc aggttctgtt ccaacacatc catagaatcc acgccgctga
     2041 tcgtggacga gacgggcagg ctcatggcat agcgcagcgc ttgctcaggc gttgctgctc
     2101 cgtggacaa
//
LOCUS       DASRKK010000836        22337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_487983, whole genome shotgun sequence.
ACCESSION   DASRKK010000836 DASRKK010000000
VERSION     DASRKK010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487983"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFC15_19965"
     CDS             complement(<1..1569)
                     /locus_tag="VFC15_19965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM12491.1"
                     /translation="MKGRIISHYEIEHEIARGGMGVVYRARDLSLNRFVALKALPVFT
                     SSDPIARMRFEREAQAASALNHPNIVTIYDLISDEHASYIVMEFIEGKTLGETIPLSG
                     LPPEQAIRYALLMADALGCAHEAGIVHRDLKPQNVLISKRDHLKILDFGVAKLKSEPS
                     GQLATYENLTRPGAFLGTTYYAAPEQFSAGEIDQRTDIYSLGVVFYQMLSGRVPFYAD
                     NPLELLQAIYHEQPKPLRNTHRHFPPIADQVIARALHKQPQGRYPTMAAFAEDLEAAK
                     EAFRQAQMESSSMGTQVSDAPIAVPPAGSSRSSYPPVWIEPPRAGHEKSSIAVLPFRS
                     LSSDKDDEYMAMGIGSEISSALSRVPGIRVASNLSTFRYQADVPDLQEIATKLKIRYV
                     LTGSLRRSGKRIRVMGELADVLASSVLWSRTYERDLEDLFKVQEEIAESIVRATGGEL
                     IRAGSEHADKASAHELDAWGLVRKAYHFWNYGFHPAGIGESLDLLRRAIQLDPGYASA
                     YAFLALYLIERVVLV"
     gene            complement(1950..2414)
                     /locus_tag="VFC15_19970"
     CDS             complement(1950..2414)
                     /locus_tag="VFC15_19970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM12492.1"
                     /translation="MSAKLKKSLATAGVLERCSSKHHLFEVDQENQGVYLILKGRVCL
                     SVKDAEKLDRIFSPGSLLGIPATFTGRPYSLAAVAVTDTEVLHVEREAFLVLMAQQPE
                     LCREATDMLSREVTFIQAALAERRRQKSNSNSDSCNSVPKKHEVTSFNRRVS"
     gene            2698..3384
                     /locus_tag="VFC15_19975"
     CDS             2698..3384
                     /locus_tag="VFC15_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM12493.1"
                     /translation="MRILVVEDDAPLASFLSKGLEAEHYAVDIASDGEDARWLACESD
                     FDLMILDLNLPKLDGIAVLQAVRPQKPSLPVLVLTGRSQLEDRVQSLDSGADDCLLKP
                     FSFTELSARVRALLRRHPFTTETVLKIADLELNRIERTVKRAGKRIELTSKEFGLLEY
                     LFRNAGHRITRSMIVEHVWNLSFDTGTNIVDVYINYLRNKIDDGFSPKLIHTVRGVGY
                     ELRCPVEGAA"
     gene            3381..4049
                     /locus_tag="VFC15_19980"
     CDS             3381..4049
                     /locus_tag="VFC15_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12494.1"
                     /translation="MTAASTSDQAAQRTERDLVSELFHAISQPLTALECGLELSLRRD
                     KTATQLRARVKSALVAAKLLHQRLSEAKVLQDAAEPGDTSQAVALETLLLELREDFLP
                     VAESAKVNLCLMCETATVRGNEARFRNGFFYLFEFLLRTCPSHRTVSIRARRASLTTL
                     EISFRNCDLGGFEFPESMPSAAPPDLGWRVAQRTFQAAGGDLVLTQNQSGQFAGYVRL
                     LLAN"
     gene            4558..5295
                     /locus_tag="VFC15_19985"
     CDS             4558..5295
                     /locus_tag="VFC15_19985"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HZM12495.1"
                     /translation="MASEQIDTPKNQCTEEEFEKIVLEQMPQVKYIARRIHEHLPQHV
                     SFEDLVHAGIVGLIDALNKFDANKNVKFSSYAKFRIRGAILDSLREMDWSPRDLRRKS
                     RRIDIVMQKLQAELGRAVSGPELAKEMGLSLTELQHLLNEIRGLEIGSLQCKSYEDGQ
                     ETDLAQNIPGPPDKDPLTLCLADERNERLQKAIAHLPEREQQVLMLYYQQELTMREVG
                     ELMGVGESRVSQIHTLAVEHLRSVISN"
     gene            5538..6665
                     /locus_tag="VFC15_19990"
     CDS             5538..6665
                     /locus_tag="VFC15_19990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZM12496.1"
                     /translation="MTETNSRRTFLVTLGRVLLLAELAPRVFADTATQFSPEIAELYR
                     KSIVIDTLCSPFASDEGLPDAAAIESVRRSGITAINFTVSAPTFEGTIDNLAYVDALV
                     EQSPDVFTVIRQYSDITRAKTEAKIGIMPGFQYTGFLEADPSRIETFRQLGIRIMQLT
                     YNNRSAFGDGCLEPGNAGLSKAGHAAVRKMNEIGVAIDLSHSGYRTTSEAIEASAKPV
                     LITHSGCAAVYAHPRSKPDEILKALADRGGYFGVYLMPYLVASPTVPTREHVLDHLVH
                     AINVCGADHVGIGSDGSIQGVRLTTEQQKAFAEDIARRKKLGIGAPGEDRYPYVPDLS
                     GPDHMEIIVAELKKRGQPGSVVEKVLGANFQRVIGEIWSTK"
     gene            6921..8879
                     /locus_tag="VFC15_19995"
     CDS             6921..8879
                     /locus_tag="VFC15_19995"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZM12497.1"
                     /translation="MLGTAWYPEQWPESRWDADLVLMQKAGIHMVRVGEFAWSRMEPT
                     EGQYDLDWLDRAITAAAKHGIVTVIGTPSAAPPAWLTQQHPETLRTLEDGRKDQHGNR
                     QQFNFTNPKYRQLARAMAEQLAKRFGHDPSVVGWQIDNEYANVSFDADTRAQFQQWLK
                     TQYGTLDNLNTRWTTSYWSETYFDWNQVPIQINYGNPGLLLNWMRFVSDTWRSYQKNQ
                     LEVIRSNADARQFITTNMMGWFNGYDHYTVAQDLDLAAWDDYVGQGHLDPVKNGAAHD
                     LTRGFKGKNFWVMETQPGGVNWASINNWLDKGEVRAMAWHDIGHGADTVSYWQWRSAL
                     NGQEQYHGTIVGPDGMPDPLYEEIAQIGREFEKASPALAGTSVKSEVAILHSYDSRWA
                     IEWQKHNKNYDPVEEILSYYGPLRAIAQSVDIVPPTASLSQYKLVVAPGLNMLTDTAA
                     ENLIKYVNNGGHLVLGQRSAMKDADNALWPQRQPGPLTELLGGRVEQYYALVDAVPVE
                     GKWGAGEGQLWAELLSAKEPDVEVLMRYGKSNGWLDGLPAAITRKVGKGRITYLGAWL
                     DKKTMAQAANWMTETSNVKPALGPVPDGVEVYPRYGAKGAVYILVNFSKAPRTVRLPT
                     TMHDVLSGGSVQSLTLPYYGVGVLASEK"
     gene            complement(9004..9294)
                     /locus_tag="VFC15_20000"
     CDS             complement(9004..9294)
                     /locus_tag="VFC15_20000"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM12498.1"
                     /translation="MKRFISSLAVLALMSGMAFAAEDGAAIFKAKCAMCHGAGGEGKS
                     GPALKGTSLTAAQIADVLTNGAPCKKAPHGKALAGFTADQATAVAAYVKSLK"
     gene            complement(9649..11523)
                     /locus_tag="VFC15_20005"
     CDS             complement(9649..11523)
                     /locus_tag="VFC15_20005"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM12499.1"
                     /translation="MSEQRLPRLLHGFVVLAVVIGILSLSGLAAPSGLPEIAKIEPPN
                     WWTNFVSPVMVLLYGENLGDAKISASYPGVKIEKTLLQPDGKHAFVWLKVAKTAKPGI
                     VTLAVKTAAGATTAALPLLERASQTGRFQGITRDDVIYLIMPDRFADGDSANNMPRGA
                     TPGTYDRQAAKAYHGGDLKGIQDHLSYFKDLGVSTLWLNPIFDNDNSSETYHGYGATD
                     LYAMEDHFGTLQDFQQLVDAAHTQGIKILLDMVPNHVGPKHPWATSQPAPNWLHGTTT
                     DHINTDYEYAPVTDPHAVEKNYRSALDGWFANVLPDLAQENPLTAEYLEQNAYWWMET
                     GGVDGFRIDTFPYVPRTFWTRYLQGLQATYPNFFAVGEVYNFDPTVVSYFAGGQKGFD
                     GIDTHLTTPFDFPMNSAIREVVNHGASAKKIVDVLRQDRLYPHPELLVTFIGNHDMKR
                     FVTDAGGSTVKLKLGLSLLATIRGIPQLYSGDEIAMPGGEDPDNRRDFPGGFPGDQRD
                     AFTQGGRTPDEQDVYSHVQGLLRLRREHPALCVGEQKHIVVTDDYYVFTRETTGERLM
                     VVFYNGDTTKSVTVELIDTTIANAKGFMPLNTAPEVRLQGSQMQLQLAPQSVAVYRVE
                     "
     gene            11741..13093
                     /locus_tag="VFC15_20010"
     CDS             11741..13093
                     /locus_tag="VFC15_20010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM12500.1"
                     /translation="MIQKPRKSFWQIWNMSFGFLGIQFGWGLQMANMSAIYEYLGARA
                     DQIPILWLAAPLTGLLVQPIIGHASDNTWTRLGRRRPYFLTGAILSSCALILMPRSST
                     LWMAAGLLWILDSSINISMEPFRAFVADLLPEEQRTQGFAMQSLFIGLGAVVASSLPW
                     LLTNYFHLRSDTSGHAIPLTVRLSFYVGAAAFFTAVLWTILSTKEYPPPDLEAFRQKK
                     SEHRGIASHATEIFTAIGNMPVTMRQLAPVQLLTWLGLFCMWLYFPVAVARNVFGATD
                     QNSPVYTRGVEWGGVCFGMYSLVCFAFSFFLPKLAKAVGRKHTHSLCLLCGAVGLVSV
                     AVIHDKNLLLLSMAGVGIAWASTLSMPYSVLAGSLPAEHTGVYMGIFNFFIVLPEIIA
                     SLGFGWVMNHLLHNNRLAAVIAGGVFMALAALLMQRVIDPGAVVIEEAVPLPGDAIAI
                     "
     gene            13174..14697
                     /gene="malQ"
                     /locus_tag="VFC15_20015"
     CDS             13174..14697
                     /gene="malQ"
                     /locus_tag="VFC15_20015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZM12501.1"
                     /translation="MSFPRSSGILLHPTSLPGPYGIGELGPEAHRFADFLKDAGQRIW
                     QLLPLGPTGYGDSPYQCFSAFAGNPLLLSLDTLVKRGYLSQDDLETKPKMPRESVDFG
                     TLIKWKLPLLRKAAAGFQISATAADREAYEVFCHSHAAWLDEFALFMALKEVHDYVMW
                     TLWEPELALREPAALDKARNDPHGQIECNKFIQFEFDRQWSDLKAHCERNGIRIMGDV
                     PIYVAQDSSDVWAAPGMFDLEANGTPRVIAGVPPDYFSATGQCWGNPIYRWDVHAQTG
                     FQWWIARFRRAFEMLDMIRLDHFRGFEAYYEIPGTATTAVDGRWVKGPGAALFEAVEA
                     ALGNLPILAENLGVITPEVEGLRTRFGFPGMAILQFAFGKDPQAPDFKPHNYPRHRVA
                     YTGTHDNDTVVGWWSSTGAGDSTRTLEDVEKEMEFARRYLNTDGSDIHWAMIRTLMAS
                     VADTVIFPLQDVLGVGSEGRMNLPGTSSGNWHWRYRAEELTLGIGSRLKLLVQTYDR"
     gene            14818..16071
                     /locus_tag="VFC15_20020"
     CDS             14818..16071
                     /locus_tag="VFC15_20020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZM12502.1"
                     /translation="MAAPWAHRYAQRTKTVKSSIIRELLKLTTNPEIISFAGGLPAPE
                     VFPTERFREACNRVLSTQATAALQYGPSEGYRPLRELIVAQMAKYGIVATVDNVLITS
                     GSQQALDLIAKLLINRGDRILVESPTYLGALQAFDLMGAEYVTVPIDGLGLQTRLLEG
                     ALRSGPKFMYVLPNFQNPGGVTLSEDRRTQLVWLSDKYGIPIIEDDPYGQLRYEGEHI
                     KPLVVLDRANVLRDNGYQLGNVIYLSTFSKVLAPGLRLGWIVAPPDVITRLVQLKQCA
                     DLHTSTFTQMVGYEVAKDGFLDEHVKTIRQCYKERRDAMLAALNDYFPPEVTWTRPQG
                     GLFLWVTMPAYVDAMRLFDEAIRHNVAFVPGQPFFAGGDAGSHMRLNFSNATPEMIRE
                     GIRRLSIAVAHEVEHTAGVMSEVVV"
     gene            complement(16210..17262)
                     /gene="cydB"
                     /locus_tag="VFC15_20025"
     CDS             complement(16210..17262)
                     /gene="cydB"
                     /locus_tag="VFC15_20025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZM12503.1"
                     /translation="MSTIWFMIVAVMVAIYVLLDGFDIGAGAVHLIIARNDTERRTIL
                     RAIGPVWDGNEVWLLAAGGTLYFAFPLLYASSFSGFYLPLIIVLWLLMLRGIGIEFRS
                     HIHDSLWTSFFDVIFSIASILLAIFFGAALGNVVRGVPLNADGYFFEPLWTNFRAGTN
                     NGILDWYTVIMGVVALVTLVVHGSLYVATKTEGDLNTRARRVAFALWPVEILLTVVSL
                     AATLLVRPGILTNFSKWPIGYLVPVVVVGSLVYLLIALRQKNDKGAFLASCGYIVGML
                     VGAAFALYPMVLPASTDPNLSLTIYNTAAGAHGLSVGFVWWTLGMVLAFTYFVFIYRM
                     FRGKVQVEEGEGSHGY"
     gene            complement(17265..18638)
                     /locus_tag="VFC15_20030"
     CDS             complement(17265..18638)
                     /locus_tag="VFC15_20030"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZM12504.1"
                     /translation="MESALLIHRLQFAFTVTYHYLFPQLTMGLAPLIVILKTLALRTK
                     DETYDKAARFWAKIFGINFAIGVVTGIPMEFQFGTNWSHFSRFAGGVIGQTLAMEGCF
                     AFFLESTFLGLFLYGEKRLSKSMHWFAAFMVFVGSWASGYFIVATDAWMQNPVGYYKA
                     ADGSLQLASFWALVLNPWAWLQYTHTMSGAVITGAFVMAAVGAFYLLGNRHVEQARIF
                     VRVGVIVGLIFAVLQLFPTGDWAGKMVAQKQPVTLAAMEALFDTQNGAPLVIIGQPDM
                     AARKIDNPILVPKMLSFLTYRRWNAQVKGLNEVPERDWPQNIPLLYFSYHIMVGLGTL
                     FIAVMGIAALLLWRQKLYQARWLLWLLMLMFPLPYLANTAGWMTAEMGRQPWLVYGLM
                     RTEEGFSKMVSAGNGWFTFLGFAGMYSLLSVLFLFLVRREIEHGPEPVNVPELPKSGR
                     LNPVEVR"
     gene            19009..19866
                     /locus_tag="VFC15_20035"
     CDS             19009..19866
                     /locus_tag="VFC15_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12505.1"
                     /translation="MTSPSQQPTKRLILKDGSFQPVVKYDIQGERVHYFSAERYEWED
                     LPSSLIDWEATKKYEADFSSGKLRVHVETPEEREEREREEANSPEISPGLRLPGTGGV
                     FLLDQLDGKPELAEILQNGSELNQRADKKSVLRTAINPIGSSKHSFELKGPHAKIQSH
                     VPAPAIYVDIDEGALNDVPLAGRFRIARADAKKDVRVVGNLKVTFTGKTSQEGNFIPA
                     NVEKLGTGEWIKIVPAQDLPPGEYAVVEMLGTEEMNLYVWDFGVNRQAPANPNIWRPA
                     PAASASSTN"
     gene            complement(19893..21032)
                     /locus_tag="VFC15_20040"
     CDS             complement(19893..21032)
                     /locus_tag="VFC15_20040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZM12506.1"
                     /translation="MYSTEIGERQFYEQADISEPGHCKPVETTDNDRQLSELKIFHDV
                     AKALTSTLDLDTILQTIMEKMAAYFEPATWSLVMIDESTQEPYYAASVGRGSEGFAAL
                     RLSDGATLTEWVIAHGEALVIENVNADPLIDPDSRSEWFDGSCSVVCMPVRTGGKVLG
                     VIQLVNIDLQVYARSEMLLRTLADYAAIAIENARAVRRIQELSITDDCTGLYNARHLF
                     TVLSDEVNRSARFGYEFTLLFLDLDHFKRVNDEHGHLIGSKLLAQVGECLRENLRLVD
                     AAFRYGGDEFAILLPQTSREAGLRVARRISRVFHQRRWLLEDKIGVDLRASIGIATYP
                     AEATTPQTIVQRADEMMYTVKQAGRDNIAVSRLGIVGLEEDRAAG"
     gene            21243..22292
                     /locus_tag="VFC15_20045"
     CDS             21243..22292
                     /locus_tag="VFC15_20045"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZM12507.1"
                     /translation="MREHPSEGKILVGSFGERLRREREMRGISLDDIADATKIGTRLL
                     RALEEEHFELLPGGIFNKGFVRAYAKYVGLNEEEAVADYLQAAGETVPDPRVIAEQNS
                     SRIDRSGGDARPSHQAGFPVIPVLILLLVIAAGAGGWQIYQERVRDQEAKRQAGDPGS
                     ATAPVNATPQPANSPLAVPSSAAASGATKTEVPSTTSTTPNAGAQAGTTTPTSVDLNS
                     PGSKAGVSTANKLTVPSSEIATPKTTGATSPNLGAAGSIAANATPFEVTVRAKDSAWI
                     SIKSDGKFIVRGIIGPPDVKTIHASDQVVVWTGNAGAVEVSFNGQPVPLAGGPNQQGV
                     LVFSSHGVVSPKLAQ"
BASE COUNT         4888 a         6488 c         6320 g         4641 t
ORIGIN      
        1 caccagaacc acacgttcga tcaggtatag cgcgagaaac gcataagcgc tcgcgtagcc
       61 gggatccaat tggatggcgc ggcgcaacaa gtcgagcgat tcgccgatac cggctggatg
      121 gaagccgtag ttccagaagt gataggcctt gcgaaccagt ccccatgcat cgagctcgtg
      181 agcggaagct ttatcggcat gttcggaacc ggcgcgaatc agttcgccac cggttgcccg
      241 tacgatggat tctgcaatct cctcctgcac cttgaacaag tcctcgagat cgcgctcata
      301 ggtacgcgac cacagaactg agctcgcaag cacatcggcg agttcaccca ttactcgaat
      361 tcgcttgccg gagcgccgca gactccctgt gagcacatag cggattttca gcttggtcgc
      421 gatctcctgt aaatcaggga cgtcagcctg gtagcggaag gttgagagat tggaggctac
      481 ccggattccc gggacccgcg aaagtgcgct gctaatttcc gacccaatgc ccatcgccat
      541 atactcgtca tccttgtcgg aagacaggct gcgaaatggc agcaccgcga tggaggactt
      601 ctcgtgcccg gcccgcggcg gctcaatcca cactggtgga tacgatgacc ggctggaccc
      661 agccggcgga actgctatcg gggcatcgga aacctgcgtt cccatcgacg acgattccat
      721 ctgcgcctgg cggaatgcct ccttagccgc ttccaggtcc tcggcaaacg cagccatggt
      781 cggatatcgc ccctgaggtt gcttatggag cgcccgcgca atgacctgat cggcgattgg
      841 cgggaaatgg cgatgggtat tacgaagcgg cttcggctgc tcgtgataga tggcctgcaa
      901 taattccagg ggattatctg cgtaaaacgg cacccggccg gagagcatct ggtaaaaaac
      961 cacgccaaga gaataaatgt ccgttcgctg atcaatctcg ccggcggaaa attgctcggg
     1021 cgcagcgtaa taggtagtgc cgagaaacgc gccgggccgc gtcaaattct cgtaggtagc
     1081 cagttgtcca gacggctccg acttcagctt ggcaacacca aagtcgagga tcttaaggtg
     1141 atcgcgtttg gaaattagaa cgttctgcgg cttcagatca cggtgaacga tgcccgcttc
     1201 gtgcgcacaa cccaaagcgt cggccatcag caaggcatag cggatcgcct gctccggcgg
     1261 caatccgctc agcggaatcg tctccccgag ggtcttgccc tcaatgaact ccatgacgat
     1321 gtagctggca tgttcgtccg aaatcagatc gtaaatcgtc acgatgttgg gatgattcag
     1381 cgcggaagcg gcctgagctt cgcgctcgaa acgcattcgc gcgataggat ccgacgaggt
     1441 aaataccgga agcgccttga gcgctacaaa tcgattgaga gacaggtccc gggcacggta
     1501 cacaactccc atgccgcctc tggcgatctc atgttcgatc tcgtaatggg aaatgatccg
     1561 gcccttcatt tgcaccgtat tgcaatacct gtccgtggcg ttgctgcacc actcaacctg
     1621 gatcgtaggc gctccacgac caagttcttg cgcgcaatga ccttctctgg cccaaaaccg
     1681 tgggtcacat tatcgtcgat gcgccacacc actcgttttg cgagtccaat gccgcaacgt
     1741 tgttttccgg attggagccc cgaaaggatg gcgcaattcc aaccataacc ccacattcat
     1801 caggtggggt tgactatatc aaagcataag tcagtccgga aagaattcag attcgcgggg
     1861 gcccggtcga acattgctgg aaacgacatt ccaccgtatc ggcttgatca gaacggcttc
     1921 ggatacctac gaactagtca gttcgcgatt caggagaccc ttcggttgaa acttgtaacc
     1981 tcgtgtttct tggggacgga gttgcagctg tctgagttgg agttgctctt ttgtctgcgg
     2041 cgctcggcca gggctgcctg gataaatgtg acttccctgc tcagcatatc ggtggcttcg
     2101 cggcagagtt caggttgctg cgccatgagc acgagaaaag cctcgcgctc gacgtgcagc
     2161 acctccgtgt cggtgacggc cacggctgcc aggctgtagg gtcgtcccgt aaaagtcgcg
     2221 ggaatcccca ggagcgaacc aggggagaag attcgatcca acttttccgc gtctttgacc
     2281 gacaaacaga ctcggccttt cagtatcaga tagactccct ggttttcctg atccacttca
     2341 aacaaatgat gtttggagct gcagcgctca agcacgccag ccgttgccag cgacttcttc
     2401 aacttggcgg acaccctgag aatgtttggc ttcgacccca ctttaggcat gtcgtacttt
     2461 atgccaaata acccggcaaa ccggctgtga ccgcaatcac aggtctctgt gaagcggcac
     2521 cgaaaccacc ttccgattga ttccagaggt ttgcttgcga atgaactgcc attcccattc
     2581 cgaaccgcgc ccgaatcggc acttgaaccc gccgctcaac gtgttcttgc attgaccccg
     2641 atcatggatg ctcgaagaat gaaaggtgac attcatattt tatggagaac gatcgcaatg
     2701 cgtatcctgg tggttgaaga tgatgctccc ttggcaagct tcctcagcaa gggactggaa
     2761 gccgaacact atgccgtcga catcgcatcg gatggcgagg acgctcgttg gttggcctgt
     2821 gagagcgatt tcgatttgat gatcctggat ttgaacctcc cgaaactgga cggcattgcg
     2881 gtcctgcagg ccgtccgccc ccagaagccg tcgctcccag tactggttct tacaggacgt
     2941 agccagcttg aagatcgggt gcaatccctt gactccggcg ccgacgattg cctcctcaaa
     3001 ccgttctcat ttacggaact ttcggcgcga gtacgggctc tgctgaggcg ccatcccttc
     3061 accacggaaa ccgtgctgaa aatcgccgac ctggaactaa accgcattga acgaacggtg
     3121 aagcgggctg gaaaacggat tgaactgact tccaaagaat ttggactgct cgagtacctg
     3181 tttcgcaacg ctggccatcg cattacccgc tccatgattg ttgagcatgt gtggaacctg
     3241 tcgttcgaca cgggaaccaa tatcgttgac gtgtacatca actacctgcg taacaagatc
     3301 gatgatggtt tctcgccgaa gctcatccat acggtccgcg gagtgggata tgaattgcgt
     3361 tgcccggtag agggggcggc atgaccgccg cgtcgaccag cgatcaggcc gcacaacgaa
     3421 cggagcgaga cctggtttcg gagctgtttc acgcgattag ccagccgctg actgcattgg
     3481 agtgcggact ggagctatca ctgcgcagag acaagaccgc cactcagctt cgcgcgcggg
     3541 tgaaatcagc gctcgtcgca gcgaagctct tgcaccagcg cctctcggag gccaaggtgt
     3601 tgcaggatgc cgcggaacca ggcgacacca gccaagctgt ggcgttggag acgctcttgc
     3661 tcgaactccg ggaagacttt ctgccggttg ccgaatccgc caaggttaat ctgtgcctga
     3721 tgtgcgaaac tgcgacggtt cgcggcaacg aagccagatt caggaacgga ttcttctacc
     3781 tcttcgagtt tctgctccgc acatgcccat cccaccgcac ggtgagcatt cgcgcccgac
     3841 gcgcgagcct caccactctc gagataagtt tccgtaactg cgatttgggc ggtttcgaat
     3901 ttcccgagtc aatgccgtct gcagcgcccc ctgatttggg atggcgcgtc gctcaacgca
     3961 cctttcaagc cgccggtggc gatctggtgc ttacgcagaa ccagtccgga caatttgccg
     4021 gttacgtgcg cctgctgctt gccaattaag ctttcaaatt gggaagtgag tcctgttttg
     4081 ggcggctccc gccgctgatt acgtttccta gagtcttcta atctccaact aattttgttc
     4141 taaggcgcgc gttctacaac gtgggatatg aaggcgtttt cgtatcccgc caccgttgtt
     4201 ggttcgtgcg agaacgcgac ctggagccag ccatccccag agacggatcg cggccggcat
     4261 gatgcccctg gctgagtgat tcgacccaag gttcggccga acgctgagcc cgcagacgtc
     4321 aggcgcctgt ctgacgagct ccacccgggt gctcagtgcc atgcggtgca ggaagatctg
     4381 cgccacttca tcggcgagca actgtgtacg acggcttcgc gctgcaaggc agcgctaata
     4441 actgtccttc ttcttgttca ttcctgccgc cccgcaccac gttgccgcgc tgtccctgac
     4501 tagatgccac cgaagacgtt cccttctggg cggcacctgt gaattggaga tcgcaccatg
     4561 gcttccgaac agatcgacac ccccaagaac cagtgcacgg aagaagagtt tgaaaaaatc
     4621 gtgctcgaac agatgccgca agtgaaatac atcgcgcgac gcattcacga gcacctgccc
     4681 cagcatgttt cctttgagga cctggtccac gccggtattg taggcttgat cgatgcgctc
     4741 aacaagtttg atgccaacaa gaacgtgaag ttctcctcct acgcgaagtt ccgcatccgg
     4801 ggcgccattc tcgacagcct gcgcgagatg gattggagcc cgcgcgattt gcgcaggaag
     4861 tcgcggcgaa tcgatatcgt gatgcaaaaa ctgcaagcgg aactggggcg cgcagtctcc
     4921 gggccggaac tggcgaaaga gatggggttg tccctgacag aactacagca tttgctcaat
     4981 gaaattcgcg ggctggagat cggcagcctg cagtgtaaat cctatgaaga tggacaggaa
     5041 actgacctcg cccagaacat ccctggaccg ccggacaagg acccgctcac tctttgcctg
     5101 gccgatgaac gcaacgagcg gctgcaaaaa gcgattgcgc acttaccgga acgcgagcaa
     5161 caggtgctca tgctctatta ccagcaggaa ctcaccatgc gcgaagtagg agagttgatg
     5221 ggcgtcgggg aatcaagagt gtcgcaaatc cacacgttgg cggtcgagca tttgcgttct
     5281 gtgatttcga actaaccgtc attctctgtt cgtcgttctc agactagcgc cgggcgggat
     5341 ccttcagccc ggcgcaattt ccctgttcca taacaaatca accgacccga aatgacagcg
     5401 gctgcttgaa ctgagatact gaagcctttc ggcgaccaga ttgatgccgg ggctcgtggt
     5461 gtttgaggtt ttggtaagcc gattcaacca ctacgcagga cgccaatttc gaattgaatc
     5521 gagagtcgca ttcacggatg accgagacga acagccgcag aacttttctg gtcaccttgg
     5581 ggcgtgtgct cctgttggcg gaactcgcgc cgcgggtgtt cgcggatacc gcgacgcaat
     5641 tctcgccgga gattgcggaa ctctaccgca agagcatcgt gatcgacacg ctctgcagcc
     5701 cattcgccag cgacgaagga ctgccggacg cggctgcgat cgagagtgtc cggcgctccg
     5761 gcattactgc catcaacttt accgtttcag ctccgacgtt cgagggaacg atcgacaatc
     5821 tcgcctatgt ggatgcgctg gtcgagcaat cgcctgacgt tttcaccgtc ataaggcagt
     5881 actccgacat cacgcgcgcg aaaacggaag ccaagattgg aatcatgccc ggctttcagt
     5941 acaccggttt cctcgaggcc gatccatcgc gcatcgaaac ctttcggcag ctgggcattc
     6001 gtatcatgca actcacctac aacaaccgca gtgccttcgg ggatggttgc ctggaaccgg
     6061 gcaatgccgg cctgagcaag gcgggccacg ctgcggtccg caagatgaac gagatcggcg
     6121 tcgctatcga cctgagtcat agcggctatc ggaccacgtc ggaggccatc gaagcatcag
     6181 ccaaaccggt actgatcaca cactcgggat gcgccgccgt ttacgctcat cccagaagca
     6241 agcccgacga gatactcaag gccttggccg atcgaggagg ctacttcggt gtctacctca
     6301 tgccctattt ggtggcatcg ccaacggtgc caacgcgcga acacgttctt gaccacctcg
     6361 tacacgccat caatgtctgc ggcgctgacc atgtcggcat tggatcggat ggctcgatcc
     6421 aaggcgtccg gttgactacg gaacaacaga aagcgtttgc cgaggacatc gcacgccgca
     6481 aaaagctcgg catcggggcc ccgggcgagg accgctatcc ctatgtgccg gacctgtctg
     6541 gtccggatca catggaaata atcgttgctg aattgaagaa gcgcggccag cctgggtcgg
     6601 tggttgagaa agtgctgggc gcgaactttc agcgtgtgat cggcgagatc tggtcaacca
     6661 aatagccaag gccggcgtcg cagcatatgg agcaaccgtc acttggtccc gcacgcgtag
     6721 aatgtccggt gctgcctcgg ttcgcatcgc agcgcccatt cgcatttcga aatccaattg
     6781 aagttcatgg agagaagatc atgaacccgt tgaatcgccc cgctcgatca cttctttttc
     6841 ctttcgtttt aatcggtcta gttgcatttg gatttctggc tctcaatgct caaccagcat
     6901 tctctaagcc gcccgcgata ttgcttggca ccgcttggta tccggagcag tggcccgagt
     6961 cgcgatggga cgccgatctt gtactgatgc agaaggcagg catccacatg gtgcgcgtcg
     7021 gcgagtttgc ctggagccga atggagccca cagaaggcca atacgatctg gactggctcg
     7081 accgtgccat tacagcggct gcgaagcatg gcatcgtcac cgtcatcggc acgccgtcgg
     7141 cggcgcctcc agcatggctg acgcagcagc atcccgaaac gttgcgcacg ttggaggacg
     7201 gccgcaaaga ccagcacggc aatcgccagc aattcaactt caccaatccc aaataccgcc
     7261 aactggcgcg ggccatggcc gagcaattgg ccaaacgttt cggccatgac ccaagcgtag
     7321 tgggatggca gatcgacaac gagtacgcca acgtttcgtt cgacgccgac accagagcgc
     7381 aatttcaaca gtggctgaaa actcagtacg gcacgctcga caacctcaac acgcgttgga
     7441 cgacctctta ctggagcgag acttatttcg attggaacca ggttcccatc cagatcaatt
     7501 acggcaatcc cggactgctg ctgaactgga tgcgcttcgt gagcgacacc tggcgcagct
     7561 atcagaagaa ccaactcgaa gtgattcggt ccaatgcgga cgctcggcag ttcatcacca
     7621 ccaacatgat gggatggttc aacggctacg atcattacac cgtcgcgcaa gatctcgacc
     7681 tcgcagcatg ggacgactac gttggtcagg gacaccttga tccggtgaaa aacggagcgg
     7741 cccacgacct gacccgcgga ttcaagggca agaatttctg ggtgatggaa acccaaccgg
     7801 gtggggtgaa ctgggcatcg atcaacaatt ggctcgacaa gggcgaagtg cgcgccatgg
     7861 cgtggcatga cattggacac ggcgccgaca ccgtgagcta ttggcagtgg cgcagcgcac
     7921 tgaatgggca ggaacaatat cacgggacca tcgttggccc ggacggcatg cccgatccgc
     7981 tctacgaaga aatcgcacaa atcgggaggg agttcgagaa agccagtccg gctctcgccg
     8041 ggacctccgt taagtcggaa gtcgccatct tgcattcgta cgatagccgc tgggcgattg
     8101 aatggcagaa gcacaacaag aattacgatc cagtcgagga aatcctcagc tactacggtc
     8161 cgcttcgtgc catcgcgcag tcggtggaca tcgtcccgcc gacggcgtcg ctaagccaat
     8221 acaaattggt ggtcgctcct gggctgaata tgttgacaga caccgcagcg gaaaatttaa
     8281 tcaaatacgt taataatggc ggacatctcg ttcttggaca gcgctctgcc atgaaagatg
     8341 ccgacaacgc attgtggccg cagcgccagc cggggccgtt gaccgaatta ctgggaggcc
     8401 gggtggagca gtattacgcg ttggttgatg ccgttcccgt tgaaggtaag tggggtgcgg
     8461 gggagggcca gctatgggcg gagttgctca gcgccaagga gcccgatgtc gaagtcctga
     8521 tgcgctacgg caaaagcaac ggctggcttg acgggctacc cgctgcgatc acgcgcaagg
     8581 tgggaaaagg ccgaatcaca tatctcggcg cgtggctcga caagaagacc atggcgcagg
     8641 cagcgaactg gatgacggaa acaagcaacg taaagccggc tctcggtccc gttcccgatg
     8701 gagtcgaggt ctatcctcga tacggcgcaa agggcgcggt ctatattctt gtcaacttct
     8761 caaaggcacc gcggaccgtc aggttgccta cgaccatgca tgatgtgctc agcggcggat
     8821 cggtccaatc cctaacgctg ccgtactacg gagtcggggt gctggcatcg gagaaatagc
     8881 aagcgaatcg ccattgcaga ccactggccg ggaaattgac gactgaagga cattggcctg
     8941 cgatgatcat ggaaaaagcc ggtcgcgggg accggcttta cgctttcaaa gaacagcacg
     9001 cgactacttg aggctcttca cgtacgcagc aaccgcggtt gcctggtcag cggtgaaacc
     9061 ggccaaagcc tttccgtgcg gagctttctt gcaaggtgcg ccattggtca gaacgtcagc
     9121 gatctgggcg gctgtgagcg aggttccctt caatgccgga ccggacttgc cttctccacc
     9181 cgcgccatgg cacatggcgc actttgcttt gaaaatcgcc gcgccgtctt ctgcggcaaa
     9241 agccattccg gacatcagtg caagtacagc taaactcgag atgaaacgct tcatgggatc
     9301 aaagtctcct tagtttttat gtttcgctaa ttactgactg caggcccttc gccggctgac
     9361 gaatccgccg tctgagcctc gagcttccga aggcaccagt gttggcttcg caacaaccgg
     9421 gtattctaga gcggtcaacc tgtggtgcaa ttgccgaact cttggcgcta ggcagacggc
     9481 gatcgtgatt gctggccacc attcacgcta gtttcactgt atcgggatgt tgcccagcta
     9541 gactgtgacc gccgtcacct tcattcggga atttgaccac cctacgacac cgctttctgt
     9601 ggccgatcac acgtcccgac ggactgtccc acgcgccggc tccatccgtc attcgactct
     9661 atagaccgca acgctctgcg gagcgagttg caactgcatt tggcttccct gcagtcgcac
     9721 ttcgggagct gtgtttaggg gcatgaatcc cttggcgttc gcaattgtgg tatcgatcaa
     9781 ctcaacggtg acgctcttgg tggtgtcacc gttgtagaaa acgaccatca agcgctcacc
     9841 cgtggtctct cgcgtgaaga cgtaatagtc atcggtcacg acgatgtgct tctgctcgcc
     9901 aacgcacaag gcgggatgtt cacggcgcag cctgagcagt ccctgtacat gcgagtaaac
     9961 gtcctgttcg tcgggagtgc gtccaccctg cgtaaacgca tcgcgctggt cacccgggaa
    10021 acctcccggg aaatcgcgtc ggttgtcggg gtcttcgccg cccggcatgg caatttcgtc
    10081 gcctgaataa agttgcggaa ttccacggat ggtcgcgagc agcgacaagc ccagcttcag
    10141 cttgacggtc gatcccccgg cgtcggtgac aaaacgtttc atgtcgtgat tgccgatgaa
    10201 cgtcaccagc aattcaggat gtgggtatag ccggtcttgt cgcagcacat ccacaatctt
    10261 ctttgccgat gctccgtgat tgactacttc gcggatggcg gagttcatgg ggaaatcgaa
    10321 gggagtcgtc aggtgcgtgt cgatgccgtc gaagcccttc tggcctccgg cgaaataaga
    10381 caccaccgtc ggatcaaagt tatagacttc gccgactgca aagaagttgg gatacgtggc
    10441 ttgcaaccct tgcaggtatc ttgtccagaa ggttcgcggg acgtagggga aggtgtcgat
    10501 gcggaagcca tcgaccccgc cggtctccat ccaccagtaa gcgttctgct cgaggtattc
    10561 ggccgtcaac ggattctcct gcgccaggtc gggtagaacg ttcgcgaacc agccatcgag
    10621 cgcgctgcga taattcttct ccacagcatg cggatcggtg accggagcat attcgtaatc
    10681 tgtgtttatg tgatcggtgg tggttccgtg cagccagtta ggcgccggtt gcgacgtggc
    10741 ccagggatgt ttcggcccaa cgtggtttgg aaccatgtcg agcagaatct tgattccctg
    10801 cgtatgagct gcatcgacaa gttgctgaaa atcctgcaaa gttccgaagt ggtcctccat
    10861 cgcgtaaaga tcggtggcgc cgtagccgtg gtaggtctcg ctcgaattgt cattgtcgaa
    10921 aataggattc agccacaacg tgctgacacc cagatccttg aaataggaca agtgatcctg
    10981 aatgcccttg agatctcctc cgtgatacgc cttggcggcc tgccgatcgt acgtgcctgg
    11041 agtcgcccct cgcggcatat tgttggcgga atcgccatca gcgaaacggt caggcatgat
    11101 gagatagatc acgtcatcgc gggtgatccc ttggaatcgc cctgtctgtg acgcccgttc
    11161 caacaacggg agcgccgcgg tcgtggcccc ggccgcagtt ttgaccgcca aggtcacgat
    11221 cccgggcttg gcagttttcg ctaccttcag ccaaacgaac gcgtgctttc catcgggctg
    11281 caggagtgtc ttctcgattt tgacgccggg atacgaggcc gatatcttgg cgtcgcccag
    11341 gttttctccg tagagcagaa ccatcaccgg cgacacgaaa ttggtccacc agttcggggg
    11401 ctctattttg gcgatctccg gaaggcccga cggagcggca agtccggaca gcgacaggat
    11461 accgataacg acggcaagaa cgacgaagcc atggagaagt cgaggaagcc tttgctcgga
    11521 catgttcatt ctttgcagcc tctgagatgc acccgctcaa gaagcgatag tattggagcc
    11581 tcaattgcag cgcaaaccgg gccacgccaa ccccgacaat ctgcaatcgg tgacgtccgg
    11641 cctttctcta tgcattcttt tggaacttct gatgcaatcg gagtacagtt tcggatgatt
    11701 cactcgccgg cacggagaag tgagcaacgg aactagcgca atgattcaaa aaccacgcaa
    11761 gagcttttgg cagatctgga acatgagttt tgggttcctc ggcatccagt ttggctgggg
    11821 actgcagatg gccaacatga gcgccattta cgagtatctg ggcgcgcgcg cggaccagat
    11881 tccgatcctg tggcttgcag ccccgctcac cggtctgctg gtgcagccga tcatcggcca
    11941 tgccagtgac aatacgtgga cgcgcctcgg acgccgcaga ccgtatttcc tcaccggcgc
    12001 catcctcagt tcgtgcgccc tgatcctgat gccgcgatct tcaacgcttt ggatggccgc
    12061 cggcctgctt tggatattgg attcgtcgat caatatcagc atggaacctt tccgcgcctt
    12121 cgttgccgac ttgctgccgg aagaacagcg cacacagggc ttcgccatgc agagcctgtt
    12181 catcggactg ggcgcggtcg tcgcatcgtc cctgccctgg cttctgacaa actatttcca
    12241 tctgcgctct gacacttcag gccatgcgat tcccttgacg gttcgtcttt cgttttacgt
    12301 cggggcggca gcctttttca ccgctgtcct ctggaccatt ctgtccacca aagaatatcc
    12361 accaccagac ctcgaggcct ttcgccagaa gaagtcggag caccgtggaa ttgcgagtca
    12421 cgccacggag atttttaccg cgatcggcaa catgcccgtc accatgcgcc agttggctcc
    12481 cgtgcaattg ctcacctggc tgggactctt ctgcatgtgg ctctacttcc cagtcgctgt
    12541 ggcccgcaat gtctttggag ccaccgacca gaactcaccc gtgtacaccc gcggtgtgga
    12601 gtggggcggc gtctgctttg ggatgtattc cctggtttgt ttcgcgtttt cgtttttcct
    12661 tccgaagctg gcgaaggccg tagggcgcaa acacacccac agcctttgcc tgctatgcgg
    12721 cgcagtcggg ctggtgtccg tcgcggtcat ccacgacaag aacctgctgc ttctgtccat
    12781 ggccggcgtc ggaattgcct gggcgagtac gctctccatg ccttactcgg tgctcgctgg
    12841 gtcgctgccg gcggagcaca ctggcgttta catgggaatc ttcaacttct ttatcgtgct
    12901 gccagagatc atcgcctctc tcggcttcgg ctgggtgatg aatcatttgc tgcacaacaa
    12961 tcgcctcgct gctgtgatcg caggcggcgt gttcatggcg cttgcggcct tgctaatgca
    13021 gcgcgtgata gatcctggcg cagtggtgat tgaagaagcg gtgccgctgc ccggcgatgc
    13081 gatcgcgatt taactccgtc gtgaatgcgt gatgcggccg tccttgatgg ccgctgcttt
    13141 accattacgg gttctcacca taggtcaaac agcatgagct tcccacgatc cagcggaatc
    13201 ctgttgcacc ccacttctct tcccggtccc tatggcattg gagaattagg cccagaggca
    13261 catcggtttg cggatttcct gaaagatgcg ggtcagcgga tatggcaact gctgccgctc
    13321 ggacccacag gctacggaga ttctccttac cagtgcttct ctgctttcgc cggaaatcct
    13381 ctgctgctca gtctcgacac gctggtgaag cgtggctacc tttcgcagga cgatctggag
    13441 accaagccca agatgcctcg cgagtccgtg gattttggga cgctaatcaa gtggaagctt
    13501 ccgctgctgc gtaaagccgc cgcggggttc caaataagtg cgaccgccgc ggaccgtgaa
    13561 gcctacgaag tcttttgtca cagtcacgct gcatggctcg acgagtttgc actcttcatg
    13621 gcgctgaagg aagtccacga ctacgtcatg tggacactat gggaacctga gcttgccttg
    13681 cgcgaaccgg cggcgctcga caaagcacgt aacgacccgc acggacagat cgagtgcaac
    13741 aagttcatcc agtttgaatt tgatcggcaa tggtcagacc ttaaagccca ctgcgaacgc
    13801 aatggcatcc gcatcatggg cgatgtgccc atctacgtcg cacaggatag ctcggatgtg
    13861 tgggctgcac ccggcatgtt cgatctcgaa gccaacggca cgccgcgagt catcgccggc
    13921 gttccgcccg attacttcag cgcgacgggg cagtgctggg gcaatccaat ttatcgctgg
    13981 gacgtgcacg cccagacagg tttccagtgg tggatcgcgc gctttcgccg tgccttcgaa
    14041 atgttggaca tgattcgcct cgaccacttt cgcggattcg aggcgtacta cgaaatcccc
    14101 ggcacagcca ccaccgccgt cgacggccga tgggtaaagg gcccgggcgc ggcgttattc
    14161 gaggcggtag aagcggcgct cggtaacctg cctatcctcg ccgagaatct cggcgtcatt
    14221 acgcctgaag tggaaggact gcgcactcgt tttggttttc ctggcatggc tatcctgcag
    14281 tttgctttcg gcaaggaccc tcaagcgccg gacttcaagc cgcataacta cccacgtcat
    14341 cgcgtggcgt atacgggcac ccacgataac gacaccgtcg tgggatggtg gtcgagcacc
    14401 ggcgccggcg acagcacgcg cacattagag gatgtcgaga aggaaatgga attcgctcga
    14461 cgctatctca acactgatgg cagcgacatt cattgggcaa tgatccgcac cctgatggcg
    14521 tcggtcgccg acactgtgat ctttccccta caggacgtac tgggagtggg cagtgaaggc
    14581 cgaatgaacc tccccggaac gtcgtccggt aactggcact ggcggtatcg tgccgaggaa
    14641 ctgacgcttg gaattggctc ccgccttaag ttgctggtcc agacctacga ccgctagtcg
    14701 atgaaacaaa tgattcattc tgttgcccct gtgaaacatg acccggggaa cggtgtcctg
    14761 cgtcacagac ggctctccgg acagcggtga gaatagggtg taaatcgggg gtgaaccatg
    14821 gccgcaccct gggcccatcg ctacgcacaa cgcaccaaaa cagtaaaaag ttccatcatc
    14881 cgtgagttgc tgaaactcac gaccaacccc gaaattattt ccttcgccgg aggactgccg
    14941 gctccggaag ttttcccgac cgagcgcttc cgcgaagcgt gtaaccgggt actgtcaacc
    15001 caggcgacgg ctgcactgca gtacgggccg tcggaaggct atcgtcccct gcgcgagttg
    15061 atcgtggctc agatggccaa gtacggcatt gtcgccacgg tggacaacgt tctcatcacc
    15121 tcgggttcgc aacaggcgct cgacctcatc gccaagctgc tgatcaatcg cggcgaccgc
    15181 attttagtgg aatcgccaac ctacctcgga gcactccagg cattcgatct tatgggtgcg
    15241 gagtacgtga ctgttccgat cgatggtctg ggactgcaga ccaggctgtt ggaaggcgcg
    15301 ctgcgctccg gcccgaaatt catgtatgtc ctgccgaact tccagaaccc gggcggcgtc
    15361 acgctctcgg aagaccggcg cacgcagttg gtgtggctgt cggacaagta cggaatcccc
    15421 attatcgaag acgatcccta cggacagcta cgctacgaag gcgagcacat caaaccgttg
    15481 gttgtgctcg accgtgcgaa tgtgctgcgt gacaatggct accagctcgg caacgtcatc
    15541 tacctgagca cattctccaa ggtgctggcc ccaggacttc ggctgggctg gattgtcgct
    15601 ccccccgacg tgatcaccag actagtgcag ctgaagcagt gcgccgacct gcataccagc
    15661 acctttaccc agatggtcgg ctatgaagtt gctaaagacg gattcctcga cgagcacgtc
    15721 aagacgatcc ggcagtgcta caaggagcgg cgcgacgcca tgttggcggc cttgaacgat
    15781 tacttcccgc ccgaggtcac gtggacgcgt cctcaggggg gattgttcct gtgggtcacg
    15841 atgccggcgt acgtcgatgc catgcgccta ttcgacgaag cgattcgtca caacgtggcc
    15901 tttgttcccg ggcagccctt cttcgccgga ggcgatgcag gttcgcacat gcggcttaac
    15961 ttctcgaatg ccacgcccga gatgatccgc gaaggcattc gccgcttgtc gatcgccgta
    16021 gctcacgaag tcgaacacac tgccggggtg atgtccgagg ttgtcgtgta ggggtttcgg
    16081 cgttcagcga cgcgcgcgat ccgaaggcaa cgcaaccaat ggataccgcg tgtggcaggt
    16141 taacatcgcg acttgaagag taaaggctcc ggaacctccg gagcctttgt gattgggggc
    16201 aatctccagt tagtagccgt gactcccttc gccctcttcc acctgcactt tgccgcgaaa
    16261 catgcggtag atgaagacaa agtaggtaaa tgccaggacc atccccagcg tccaccagac
    16321 gaagcccacg ctcaaaccgt gagcgcccgc tgctgtgttg tagatggtca ggctgaggtt
    16381 ggggtcggta ctggccggta gcaccatggg atagagcgcg aacgccgctc ccaccagcat
    16441 gccgacaatg tagccgcaag aagccaggaa ggccccctta tcgtttttct gccgcagcgc
    16501 gataagcaga tagaccaggc tgccaacaac cactaccgga acgaggtatc cgattggcca
    16561 tttgctgaag ttggtcaaaa tgcccggacg caccagcaac gtagccgcga gactgacgac
    16621 ggtgaggagg atctccactg gccacaatgc gaatgcaacc ctgcgggcac gtgtattgag
    16681 atcgccttcc gtcttggtgg caacgtataa ggagccatgc accaccagcg tgaccagcgc
    16741 cacaacgccc atgattacgg tgtaccagtc gagaattccg ttgttggtgc cggcacggaa
    16801 gttcgtccac agcggttcga agaaatatcc atcggcattc aagggcacgc cgcgcacgac
    16861 gttgcccagt gcggcgccaa agaagatcgc gagcaggata ctcgctatgg agaaaatgac
    16921 atcgaagaac gacgtccaca ggctatcgtg aatgtgactg cgaaattcaa tgccaatgcc
    16981 gcgcagcatc aatagccaca gcacgataat cagcggcagg taaaatccgc tgaagctcga
    17041 ggcatacagc aacggaaaag cgaaatacag cgttcctccg gcggcaagca gccagacttc
    17101 gttaccgtcc cataccgggc caatcgctcg caggatggtc cgccgttcgg tatcgttcct
    17161 ggcgatgatg aggtggacag cgccggcccc gatgtcgaag ccgtcgagca acacgtagat
    17221 ggcaaccatt accgcgacaa tcatgaacca aatcgttgac atggctatcg cacctccaca
    17281 ggattcaagc ggcccgattt cggcaactct ggaacgttga ccggctcagg tccatgctcg
    17341 atttcgcgcc gaaccaggaa gagaaacagg acggaaagca aggaatacat gccggcaaac
    17401 ccgaggaacg taaaccagcc gttcccggct gacaccattt tggagaagcc ttcttcggtg
    17461 cgcatcagcc cgtacaccag ccagggctgg cgtcccattt ccgccgtcat ccaccctgcg
    17521 gtgttagcaa gatagggcaa cgggaacatg agcattaaca gccacagcaa ccaacgcgcc
    17581 tggtagagct tctggcgcca cagcagaagc gcagcgattc ccatcaccgc gataaacaac
    17641 gttcccagcc caaccatgat gtggtacgag aagtaaagca gcggaatgtt ctgcggccag
    17701 tctcgttccg gcacctcgtt gagtcctttc acctgcgcgt tccaacggcg gtaggtgaga
    17761 aagctcaaca tcttggggac cagaatcggg ttgtcgattt ttcgcgcggc catgtcaggc
    17821 tggccgatga ttaccagcgg cgctccgttt tgcgtatcga agagcgcttc catcgcagcc
    17881 aatgtcacgg gttgcttctg tgcgaccatc ttccccgccc agtcccctgt cgggaacagt
    17941 tgcaacacag cgaagatcaa gcccacgatc acgcccaccc ggacgaatat acgtgcttgt
    18001 tccacgtgcc gattgccaag caggtagaac gcgccaacag ccgccatcac gaacgctccg
    18061 gtgataacgg caccgctcat ggtgtgggta tattgcagcc atgcccaggg gtttaacacc
    18121 agcgcccaga aactggccag ttgcagcgag ccgtcagcgg ccttgtagta accgacggga
    18181 ttctgcatcc aggcatcggt ggcaacgatg aagtagcctg aagcccacga ccccacaaat
    18241 accatgaacg ccgcgaacca gtgcatcgac ttgctcaaac gcttctcgcc atagaggaac
    18301 agtccgagga aagtcgattc cagaaagaat gcaaagcacc cttccatggc cagcgtttgt
    18361 ccgatcacgc ctccggcgaa gcgtgaaaag tgcgaccagt tggtgccgaa ctggaattcc
    18421 atcggaatcc ccgtcaccac gccaatcgca aaattgatgc caaaaatttt ggcccagaat
    18481 cgggccgcct tgtcgtaggt ctcatctttc gtccggagtg ccaaagtctt aagaattacg
    18541 atcagcggcg ccagtcccat ggtcaattgc gggaacagat aatggtacgt caccgtgaat
    18601 gcgaattgca ggcgatggat caaaagtgcg ctttccatgc atgtccctca gtaaaagaaa
    18661 gatgccgttc cgatagcgcc cccaaggtct ctgacgaagg gtgcgtcgcg gaccacaacc
    18721 gttctacctg atcgcgacag atcgtgccgt gaccggaatc acaagacaaa tgattgttgc
    18781 accaacaccg aagcgataac ttcaacatgg tggctgcgac aaaactcgtg ttgcgcgcgg
    18841 ataagaccag ctttccgatc cattgatggc tgaccttccc gagagaacca aaacgctctg
    18901 tcgtattttg ggtctacact aatatcgatg ttccgcctcg ccgccagtac gattctggta
    18961 ttcgctcttt gggcgaatct ggctgtaaca tcgaccgccc agacagcgtt gacatctccg
    19021 tcacagcagc ccaccaagag gctgatcctg aaagatggtt cctttcagcc cgtggtcaag
    19081 tatgacatcc aaggtgagag agttcactac ttcagtgcag aacgatacga gtgggaagat
    19141 ttaccctcgt cactgatcga ttgggaagcc acgaagaaat acgaggctga tttcagcagc
    19201 ggtaaattgc gcgtgcacgt ggaaacccct gaagagcgtg aggagcgcga aagggaggag
    19261 gccaactccc cggagatctc gcccggtctg cggttaccgg gaaccggagg ggtatttctc
    19321 ctcgatcagc ttgatggaaa acccgagttg gcggagattc ttcagaacgg cagcgaattg
    19381 aaccagcggg ccgacaagaa gagcgttctg cgcacggcaa tcaatcccat tggtagctcg
    19441 aagcattcat tcgagctgaa gggaccacac gcgaaaatcc agtcgcacgt tcctgcaccg
    19501 gctatttatg tggacatcga cgagggagcg ttgaacgacg tcccgttggc gggccggttt
    19561 cgcatcgcac gcgcggacgc aaagaaagat gtgcgagtgg tcggcaactt gaaagtcact
    19621 ttcactggca agacgtctca agagggaaac ttcatccccg cgaacgtcga gaaactcgga
    19681 acaggcgagt ggataaaaat agtgccagcg caagatttgc cgcctggcga atatgcagtc
    19741 gtagagatgc tgggaacgga agagatgaat ctttacgtgt gggatttcgg agtaaaccgg
    19801 caggcgccag ccaatcccaa catctggcgt cccgcgccag ccgcgagtgc atcctccacg
    19861 aactgacccg aaccgcagcc gcggcgctgg cactaaccgg cggcacggtc ctcttcgagt
    19921 cccacgatac caagtctact gaccgcaatg ttgtcgcgtc cggcctgctt gaccgtatac
    19981 atcatttcgt cagcgcgctg aacaattgtc tgaggcgtcg tggcttccgc cggataggtc
    20041 gcgataccga tactggcccg cagatccact ccgatcttgt cctccaggag ccatcgccgt
    20101 tgatgaaaca cgcgggagat tcgtcgcgcc acccgcaaac ctgcctcgcg tgaggtctgg
    20161 ggaagcagga tcgcgaattc gtcgccgccg tagcgaaatg cggcatcgac cagtcgtagg
    20221 ttttcgcgca ggcactcgcc aacctgcgcc agcagcttgc tgccaatcaa gtggccgtgc
    20281 tcgtcgttga cgcgtttaaa gtggtcgaga tccagaaaca gcaacgtgaa ctcatagcca
    20341 aatcttgccg accggttcac ttcgtcggag aggaccgtaa acagatggcg agcgttgtac
    20401 agaccagtac agtcatcggt gatactgagt tcctgtatgc gccgcacggc ccgggcattc
    20461 tcgatggcga tggctgcgta gtcggcaagc gttcgcagta gcatctcgct gcgggcatat
    20521 acctgcaagt cgatgtttac gagctggatc acgcccaaca ccttgccgcc ggtccgcact
    20581 ggcatgcaaa ccaccgagca gctaccgtca aaccattcgc ttcggctgtc agggtcgatg
    20641 agcgggtccg cattaacatt ctcaatcacc agcgcttcgc cgtgggcgat cacccactct
    20701 gtcaacgtgg cgccgtcaga caatcgcagc gccgcgaagc cctccgaacc cctgccaacc
    20761 gaagcggcat agtacggctc ctgagtggat tcgtcgatca tgaccagcga ccacgtcgcc
    20821 ggttcgaagt acgctgccat tttttccatg atggtctgca ggatggtgtc gaggtctagc
    20881 gtcgaagtca gcgccttcgc aacatcgtgg aagatcttca actcagatag ctggcgatcg
    20941 ttgtccgtgg tttccactgg tttgcaatga cctggctcgg agatgtcggc ttgttcgtaa
    21001 aactggcgct cgccgatttc agtagagtac atcagttcgt tggccaactc ttagccactg
    21061 ttaggtgtct atgttacagg aaaatagtgg tttatcctat actgcttagg tgcttgaact
    21121 ggttgtagtc tggattcagg aatccgcggt tgtcgggaat tccggcgtcg cgcagaaacg
    21181 gatgattggg atacttgccg tttcggtgtc catgatgcga aacctgcgca ggtagactgg
    21241 gaatgcgcga acatccaagc gaagggaaga tactagtggg ctcatttggc gaacggcttc
    21301 gtcgcgaacg cgagatgcgt ggtatctcac tcgatgacat cgccgacgcg accaagatcg
    21361 ggacacgtct gcttcgtgcc ctggaggaag aacatttcga actgcttccg ggtggaattt
    21421 tcaacaaggg atttgtccgc gcctatgcca aatatgtagg gcttaacgaa gaggaagccg
    21481 ttgctgacta cctgcaagcc gcgggcgaaa cggtgcccga cccgcgggtg attgcggaac
    21541 agaattcgtc gcgcatcgat cgttccggag gcgatgcaag accctcacac caagctggct
    21601 tcccggtcat tccagttctc attctcctgt tggtcatcgc ggccggagcg ggtggctggc
    21661 agatctacca ggaacgagtt cgcgatcaag aggcgaaacg tcaagctggc gacccaggtt
    21721 cggccacggc tcccgtgaat gcgacaccgc agcccgccaa ttccccactg gcggtgccct
    21781 cgagtgcagc agcttccggc gcaaccaaga ccgaggtccc ttctaccacc agcacaaccc
    21841 cgaacgcggg agcgcaggcg ggtacaacta caccaacctc agtcgatctg aattccccgg
    21901 gttcaaaagc tggcgtttcg accgcaaaca agctgacggt tccgtcatcg gaaatcgcaa
    21961 cacccaagac gacgggagcg acaagtccga acttgggggc ggctggctca atcgcagcca
    22021 acgctactcc attcgaagtg actgtccgtg ccaaagacag cgcctggata tcgattaagt
    22081 ccgatggaaa gttcatcgtg cgcggcatca tcggccctcc tgacgtgaag accatccacg
    22141 ctagcgacca ggttgtcgtt tggaccggca atgccggcgc ggtggaagtt tcgttcaacg
    22201 gccagcccgt ccccctggca ggcggcccca accagcaggg cgtgctggtc ttcagctccc
    22261 acggtgtcgt gtcgccgaag ctggcgcaat gaaatcgatt ttcttagtgt tgaaattttc
    22321 attgtcatga gaaatgt
//
LOCUS       DASRKK010000837         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_585656, whole genome shotgun sequence.
ACCESSION   DASRKK010000837 DASRKK010000000
VERSION     DASRKK010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585656"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFC15_20050"
     CDS             <1..442
                     /locus_tag="VFC15_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12508.1"
                     /translation="GLWAHLKSVAPGNRHLGALSISRRLATVQLGKVALVGEASGSVD
                     AVTGEGLSMAFRQAIALAEAMRVGSLQQYETAHRRIARLPRMMSTLMLAMDAHPHFRR
                     RVFRALTSEPDSFRRLLALHTGAISPLKFGFRETLSMGWHLLAA"
     gene            504..1100
                     /locus_tag="VFC15_20055"
     CDS             504..1100
                     /locus_tag="VFC15_20055"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZM12509.1"
                     /translation="MFRTGCIALFAWLVPLPSMVAAQPITVNLDPAQTKIEWTLGDVL
                     HTVHGTFKLRSGSVTFDPKSGDAGGEIIVDATSGESGNHARDKKMHKEILESQRYPEI
                     IFSPKHVMGKLADQGTSNIQVQGVFHIHGADHDLTLSLPVEKSDDAVKTSTRFVVPYQ
                     DWGMKNPSTFLLKVENKVSISISSAGHITPAGAASPAH"
     gene            complement(1183..2619)
                     /locus_tag="VFC15_20060"
     CDS             complement(1183..2619)
                     /locus_tag="VFC15_20060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM12510.1"
                     /translation="MFLRKTKSQGQSAAWRIAIWPTVAFALGSAVAFAIMYLLIARDI
                     RERSDAWLSGEAEVLADVSENTPRDALYDRLVQEVAELASREVPDTSDAYGKHQASVF
                     FLKTEPRQEPIWVGPTPKELFIPAVTSMPLLPGVPANVRVQGWKKAFRVVYHAQKDGG
                     GLYLGFADLGGAQMLDRLTERCLMVWGVTVALGFLITWLAAYRTLTRVERISETVSRI
                     GSEDLGSRLPEGPHFDEISRLSRTFNRMLDRIQSSVNQMRILTDSVAHDLKSPVTSIR
                     GSLEVALSDADQRQWRERVAEAIDGLDRLSQLLNTTLDLAEADAGALQIRKEPVELSD
                     LVKQLVDLYQPAMAERGHEVVTHLQPALIEADSSLLNRTIANLLDNEIAHLPNSCRVN
                     ISVRVKDGEAELTVEDNGPGFPPGLRSRAFERFVKGQHSTGHGLGLAFVDAVARTHGG
                     RVEISDRAEGGAVIVLTLGEAVNEKVGR"
     gene            complement(2600..3277)
                     /locus_tag="VFC15_20065"
     CDS             complement(2600..3277)
                     /locus_tag="VFC15_20065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM12511.1"
                     /translation="MRLLVVEDDSAIREFLNRGLTEAGYRVDVAPDGKSALALVDEEV
                     YDGFIVDLGLPDMDGLDVIGRCRALGHTAPVLILSARRSVDERVQGLEQGGDDYLTKP
                     FALAELLARVRNLLRRGAAPQTEAVRLRVADLELDLVRREARRGSDLVQLTTQEFALL
                     EYLVRNAGRVVTRTMILDRVWRMRIDPATNVVDVQIYRLRNKVDAEGKRPLIHTIRGV
                     GYVLKEN"
     gene            complement(3339..>3549)
                     /locus_tag="VFC15_20070"
     CDS             complement(3339..>3549)
                     /locus_tag="VFC15_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12512.1"
                     /translation="VMKLFRRERVLTQICGNNFMVLKSAPPLVAKEQHIEEFVESLGR
                     VVAEVHNSTAFWSEALGLARRAINV"
BASE COUNT          748 a         1086 c          990 g          725 t
ORIGIN      
        1 cggattgtgg gctcacctga agagtgtagc gcccggcaat cgccacttgg gagctttgtc
       61 aatcagccgt cgcctcgcga ccgttcaact cggcaaggtc gccctggtgg gagaggcatc
      121 gggttcggtt gacgcggtta cgggcgaagg gctgtcgatg gcgtttcgtc aggcaatcgc
      181 ccttgcagag gcgatgcgcg ttggcagtct ccaacaatac gaaacggcgc accgtcgcat
      241 tgcgcgcctt ccgcggatga tgtccacgct catgctggcc atggacgcgc acccccattt
      301 tcgccgtcga gtgtttcgcg cactaacatc ggagccggat tcctttcgcc gattgttggc
      361 gcttcacacg ggagccattt cgcccttgaa gtttggcttt cgagagaccc tgtccatggg
      421 atggcatttg ctggccgcat aaactcgagg cgaatcggag gagcaacaat ttgaggctca
      481 ggtctccaat tcattcaggt cgaatgtttc gtacgggttg tatcgcgctg tttgcctggt
      541 tggtgccgtt gccgtccatg gttgcggcac agccgatcac agttaatctc gatccggccc
      601 aaaccaaaat cgagtggacg ctcggggatg tgttgcacac tgtccatggc actttcaaat
      661 taaggagtgg cagcgttacg ttcgacccga aatccggcga cgctggcggc gaaatcattg
      721 tggatgcgac cagcggcgag agcggcaatc atgctcgcga caagaagatg cacaaagaga
      781 ttctggagag ccagcgctat cctgagatca ttttttctcc caaacatgta atggggaagc
      841 tcgcggacca agggacatcg aacatacagg tgcaaggcgt atttcatatc catggcgccg
      901 accatgacct cacactgtcc ttgccggttg aaaagagtga cgacgcggtc aagacgagca
      961 cgaggttcgt tgtgccctat caagattggg gtatgaagaa cccaagcacc ttcctcttga
     1021 aagtcgagaa caaggtgagc atcagcatct caagtgcggg ccacatcacc ccagccggcg
     1081 cagcctcccc agcacactag agtcttcatc gaaggaccct ggaccctagc gccagcacaa
     1141 tcatgagcag gtgcggccaa tggcgacact gctgatgcga cgttaacggc caaccttctc
     1201 gtttacggct tcgcctaacg taagcacaat tactgcgccg ccttcagcgc ggtctgagat
     1261 ctcgactcgt cctccatgag ttctggctac cgcgtccaca aatgccagtc ccagaccgtg
     1321 tccagtggaa tgttgtccct tcacaaagcg ttcgaaggca cggctgcgaa gccccggcgg
     1381 aaacccggga ccgttgtcct cgaccgtgag ttcggcttcg ccgtccttca cccgtactga
     1441 gatgttgacc cgacagctgt tcggaaggtg cgctatttcg ttatcgagga gattggcgat
     1501 tgtccggttc aacagcgaag aatctgcctc aatcagtgcc ggctgcaaat gagttacgac
     1561 ttcatgtcca cgctccgcca tcgcaggctg gtagaggtct accaactgtt ttaccaggtc
     1621 ggaaagctcg acgggttcct tccggatttg caaggctccg gcgtcggcct ccgcgagatc
     1681 gagcgtcgta ttcaacaatt gcgaaagccg atccaggccg tcaatggctt cggcgacacg
     1741 ctcccgccac tggcgttgat cggcgtccga cagtgcgact tcgagactgc cacgaatgga
     1801 ggtgaccggg ctcttcaggt cgtgggccac cgaatcggta aggatgcgca tctggttaac
     1861 cgacgactga atgcggtcga gcatgcgatt gaaggtgcgc gataggcgcg aaatctcatc
     1921 gaaatgcggg ccctccggga gccggctgcc cagatcttcg ctgccgatgc gagacaccgt
     1981 ttcgctaatg cgctctaccc gggtcagggt acggtaggcc gccaaccagg tgatcaggaa
     2041 accgagagcg acggtcacgc cccacaccat gagacaacgc tcggtcaagc ggtcgagcat
     2101 ctgcgcgccg ccgaggtcgg cgaatcccag atacagcccg ccaccgtcct tctgggcgtg
     2161 gtacacaacc cgaaatgcct ttttccatcc ctgcacgcga acattggctg ggactcccgg
     2221 gagcagcggc atgctcgtca ccgccgggat aaacagttcc tttggagtcg gacccaccca
     2281 gatcggttcc tgccgcggtt ccgtcttcag gaagaaaaca gaagcttggt gcttgccata
     2341 ggcatcgctg gtatccggca cttcccggga agcgagctct gccacctcct ggaccaagcg
     2401 gtcatacagc gcatcacgcg gggtattttc cgaaacatcg gccagaactt cagcctcccc
     2461 actgagccac gcgtcgctgc gctcgcgaat atctctcgcg atcagtagat acatgatcgc
     2521 aaaggccacc gcgcttccca atgcgaaagc cacggtcggc cagatggcga tgcgccaggc
     2581 cgctgactgc ccctgcgatt tagttttcct taagaacata gccaacaccg cggatggtat
     2641 ggattagtgg gcgcttgcct tcggcgtcta ccttgtttcg caaacggtag atctgcacat
     2701 ccaccacatt ggtcgccgga tcgatgcgca tgcgccacac ccgatcgaga atcatggtcc
     2761 gcgtcaccac ccgtccagcg ttgcgaacca ggtattcgag gagcgcgaat tcctgggtcg
     2821 tcagttgtac caggtcgctt cctctgcggg cttcgcgccg aaccagatcc aattcaagat
     2881 ccgctacccg taaccgcacc gcttccgttt ggggagcggc gccacggcgc agaagattcc
     2941 ggacccgcgc cagcagttcc gccagcgcga atggcttggt caggtaatcg tcgccgccct
     3001 gttcaagccc ctgaacccgc tcatcgaccg agcggcgcgc tgacaaaatc aatactggag
     3061 cggtatgacc cagggcgcgg cagcgcccga tcacgtcaag cccatccatg tcgggcaatc
     3121 cgaggtcgac aatgaagccg tcgtaaactt cttcgtccac gagggctaac gccgattttc
     3181 cgtccggagc aacatcgact cgatagccag cttccgtcag gccacgattc aagaactctc
     3241 gaatcgccga atcatcctcc acgactaaca gtcgcacagc tctcctcttg caatgggctt
     3301 ccgagtcaaa ggatatcgca gacagctctt ggcgtgtttc aaacgttgat cgcacgtcgc
     3361 gccagtccca gagcctcgct ccagaatgcg gtagagttat gaacttcagc cacgactcgt
     3421 ccgagcgatt ccacgaactc ctcgatgtgc tgttctttcg cgaccaacgg gggggccgac
     3481 ttcagcacca tgaagttgtt cccacatatc tgcgtaagaa ccctctcgcg gcggaagagt
     3541 ttcatcacc
//
LOCUS       DASRKK010000838         6119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_878842, whole genome shotgun sequence.
ACCESSION   DASRKK010000838 DASRKK010000000
VERSION     DASRKK010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878842"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..489)
                     /locus_tag="VFC15_20075"
     CDS             complement(82..489)
                     /locus_tag="VFC15_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12513.1"
                     /translation="MKFFAYSGTMQEVCLRLMGKKLLVALAMEGKFTSDGLQAIDGDD
                     DMLTAMARELVQNKGIGESADNLWRRVGAFLPASGQQEPMKELRAVSEGNVADIPSFP
                     PPPGADHATTPLCAFAGSHTDQSTDFAVQLPLF"
     gene            821..1870
                     /locus_tag="VFC15_20080"
     CDS             821..1870
                     /locus_tag="VFC15_20080"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM12514.1"
                     /translation="MADVRWEHYPHVGGNEVARAWLVLESNLGLAANTLDAYGRGLEE
                     YIAFSAGKDTAVVTATRDHIAAYIRYLTSRPSRRAPNVLGLDSGAGLANATLQQRITA
                     VRLFYDYLMEEGLRPNNPVGRGRYTPGKSFGGCQDRGLIPRFTKLPWIPNDQQWHAVL
                     GAARKESVRNRMMLALSYDAALRREELCGLDTCDVDPAHRTITIRAENTKTRRGRVVP
                     YSPPTGDLLAAYLRRRREFSRDRGRLFISESRRNRGRPISIWTWSKVVERISACSGVP
                     EFTTHSSRHLCLTDLARAQWDVHEIATFAGHRSIQTTLLYIHLSGRELALKLAKGMAE
                     IHAWRTRVLKEALSE"
     gene            2088..4166
                     /locus_tag="VFC15_20085"
     CDS             2088..4166
                     /locus_tag="VFC15_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM12515.1"
                     /translation="MAAIKSPRQSRSGALTVILIEMHKRQQPFWRWTPPDCLEVLCSS
                     TIDFRKRYRQVSGHARQVLAAGMYLLRLFDDFRKLGIIDRTALACRVFGRSHIENAIK
                     RVVDITVSWGYGRFQAKDVQWAVCTVLLANKSPRLEDLTLDLLQAERAITSVDYRRSS
                     IAVLSRVLTDLGIVPHPLAKVSSHSNMGKVTNGVDPVWVDWVERWQKTSTLQANSRYR
                     NFVNLLKTGRWVTTTHPELASPENWTRDSAAEWVALVCRMTIGDWTQVDQRFQKNRGK
                     PLSARARAHLLSSLSAFFRDIQEWGWVPRRFDPRRALAAPRSLRALIGPKPRVIDDDV
                     WAKLLWAGLNLQQSDLTKHHLGNHYYPLTMVKALAVAWLFCGLRGDEIRRLPVGCTRE
                     HWTSSSDPAATVCDLDVPVNKTSQAFTKPVDQIVGESVRAWEAERPQQPAAVDEKTSE
                     LVHFLFAFRSKQISATYINQTLVPILCKKAGIPDKDARGTITSHRARSTIASQLFNAK
                     EPMSLFELQRWLGHKWASSTQHYLDVSPTKLAQAFRDAGYFARNVRAIEVLIDHEVVI
                     AGKAAREPWRFYDLGHGYCAYDFFDQCPHRMACAKCSFYLPKGSSQALLLEGKAHLLR
                     MRQEIPLNAAELAAVDDGVSALNKLLAQLADVPTPAGPTPRQLRDSSLVQITAIATEG
                     SVNEPAIGEL"
     gene            complement(4555..4962)
                     /locus_tag="VFC15_20090"
     CDS             complement(4555..4962)
                     /locus_tag="VFC15_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12516.1"
                     /translation="MVLRERASHEGDNARQSRLVDIETIEESFDDNYGLAVIHGPVQI
                     EEDQRFAEPGRKAISRFGLAKRSSCISDKAVILIVNGYHDAPLHVALSRKETNPKVLC
                     SFWADSPLPKIVVVDINILEAEGKRRIGFFADR"
     gene            complement(5353..>6119)
                     /locus_tag="VFC15_20095"
     CDS             complement(5353..>6119)
                     /locus_tag="VFC15_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12517.1"
                     /translation="ARKVAGLTGTLLSGYADDLYNTLFRVDASRMVRDGFTWGSTGKE
                     RFTHEFGVIETIERTKIEDNACSRKNKTEVTIRRKPGASPLLFGKYLMDTCAFVSLED
                     ISDALPAYREDVIPVALEGALKTAYEQLEEEITATLHEHRGNRSVLSTMLNALLVYPD
                     HPYGLGTLYGNRYNPETQQRESFIIAEPEDLDESKVYPKERALIEEVKRQLERGRKCQ
                     VFAVYTNKYGRNGTAGEAAASRGGSRRRAPGQRAHP"
BASE COUNT         1255 a         1794 c         1743 g         1327 t
ORIGIN      
        1 cctctccagg cttcggcagt cttcaaggtt ttgagtttca tggcggttga ggaaggagac
       61 gcagaccccg gcgcgctgcc cctagaacag gggcagctgc accgcgaagt cagttgattg
      121 atcagtgtgg cttcctgcga aggcacacag gggagttgtt gcgtggtctg cgcccggcgg
      181 cggtgggaac gatgggatat ctgcaacgtt cccttcgctg acagctcgga gctccttcat
      241 cggttcctgc tgtccggaag cgggcaggaa tgcaccgacc cgccgccaga ggttgtcggc
      301 agattctccg attcccttgt tctgcaccag ctcacgcgcc atagcggtga gcatgtcgtc
      361 gtcgccgtcg attgcctgca gcccatcgga ggtgaacttc ccttccatgg ccaaagcgac
      421 cagaagcttc ttgcccatca ggcgcaaaca gacctcctgc atggtgccgc tataggcgaa
      481 gaacttcact tcaaccggat gcttctgccc tatgcgccac gaacgccggc tggcctgccg
      541 caaagtgtgc agggaatagc cggtttcgtg gaagaggatg gccggaaact ccagcagatc
      601 taagttacgt ggaatatgga caaccggaat cggttacgac gtacggtgaa tattttcttt
      661 tttgaaacca gtttgatctt cgcgttggac ccaggatgca gtgtttaaca gaaggaccaa
      721 agggtatcta accggataac ccccttcaga gatagttgcg acaacaccaa cttgttctgc
      781 ttccaggacc gtcaaagcta atcgggaaga ggtgaatcaa atggctgatg tgcgatggga
      841 gcactaccct catgtaggtg gcaatgaggt agcccgggct tggctggtgc tggaatccaa
      901 tctgggactt gcggcgaata cgctggatgc atacgggcgt ggattggaag aatacatcgc
      961 attcagcgca ggcaaggaca cagcggtcgt cactgccacc agggatcaca tcgccgcgta
     1021 tatccgctac ctaacgtcac ggccgagccg acgcgcaccg aacgtgcttg gactcgactc
     1081 tggcgcaggt ctcgctaatg cgactctgca gcaaaggatt acggctgttc gtcttttcta
     1141 cgactatctg atggaagaag gcctaaggcc taacaacccg gtcgggcgcg gccgctacac
     1201 tcccgggaaa agctttggcg gttgccagga ccgaggacta attccccgat tcacaaagct
     1261 cccctggatt ccaaacgacc aacaatggca cgcggtgctg ggggcggcgc gcaaggaatc
     1321 ggttcgaaac cgcatgatgc tggcgctctc gtatgacgcc gcgttgcgcc gagaagagct
     1381 ttgcggtctg gacacgtgcg atgtcgaccc cgcccatcga accatcacga ttcgagcgga
     1441 gaatacgaaa acccgccgag ggcgcgtggt gccatattcg ccgcctactg gcgatctgct
     1501 tgccgcctat ctgcgccggc ggcgcgagtt tagtcgcgac cgcggccgcc tattcatttc
     1561 cgaatcgcgg cgcaatcgcg gccggccgat ttctatatgg acatggtcca aggtcgtgga
     1621 gaggatctcg gcatgcagtg gcgttcctga gttcaccact cacagttcgc gccacttgtg
     1681 cttgacggat ctggcacgag cgcaatggga tgttcatgag atcgccactt tcgccgggca
     1741 ccgtagcatt caaacaaccc tcctctacat ccacctgagc ggtcgcgaac tggccttgaa
     1801 actggctaaa ggcatggcag agattcacgc ctggcgcacc cgggtattga aggaggcact
     1861 ctctgaatag gctctacatc aattctgcga accttcctct atcgcggccg gagatggatt
     1921 ggtcatggcc tgtggacctt cagcgatatg atcgttctcc aaaacttcgg agagatgaga
     1981 tcggccagct tgcccgcgtg gctgcgcggc agcgaggagt atcgggtcat ttctctccgc
     2041 aaatgaagga ggaccttcgc cggctcaccg atccattgga tgacgtaatg gcggccatca
     2101 agtcacctcg acaatcgcgc tccggagcgc tgacggtcat cctgattgaa atgcacaagc
     2161 gtcagcaacc tttctggagg tggacgccgc ctgattgcct ggaagtcctc tgttcctcca
     2221 caatcgattt ccgcaaacgc tatcggcagg tcagcggcca cgctcggcaa gtgctggccg
     2281 ctggcatgta cctgttgcga ctgttcgatg atttccggaa gcttggaatc attgatcgaa
     2341 cggccttggc ttgccgagtc ttcgggcgtt cacacattga gaatgccatt aagcgagttg
     2401 tagatatcac agtttcctgg ggctatggac gttttcaggc aaaagatgtt caatgggccg
     2461 tttgtaccgt cctgcttgca aacaagagcc cacggctaga agatctcacg cttgatctcc
     2521 tgcaagcgga gagagcgata acctccgtcg actaccggcg atcgagtatt gctgttctgt
     2581 cccgtgtcct taccgacctc ggtattgtcc cgcatcctct cgcgaaggtt tccagccact
     2641 cgaatatggg taaggtcacg aacggcgttg atccggtatg ggtcgactgg gtcgagcgtt
     2701 ggcagaagac gagcactctg caagcgaaca gccgttaccg gaattttgtc aacctgctca
     2761 agaccggccg ctgggtaacc accacacatc cggagctcgc gtcgcctgaa aactggactc
     2821 gcgattcggc cgccgagtgg gtggctttgg tctgccgcat gaccatcggg gattggacgc
     2881 aagtggatca gagatttcag aagaatcgtg gcaaacctct gtcggcaagg gccagagcgc
     2941 atctgctatc gtcgctgagc gcattcttcc gcgatatcca agagtggggc tgggtcccac
     3001 ggcgttttga tccccgccgt gctctcgcgg cgcctcgaag tctccgcgca ctgatcggcc
     3061 ccaagccgcg cgtcattgac gatgacgtgt gggcgaaact gctatgggcc ggcctcaatc
     3121 tccagcaatc tgatctgacc aagcaccatt taggaaatca ttattatccg ctgacgatgg
     3181 ttaaagcgtt ggccgtcgcg tggttgttct gcggccttcg cggcgatgag atcaggcgcc
     3241 ttcccgtcgg ttgtacacgg gagcactgga cgtcctcctc cgatccagcc gccaccgttt
     3301 gcgacctgga tgttcccgtg aacaagacca gccaagcttt cacaaagccg gtggaccaga
     3361 tcgtaggcga atcggtacgc gcctgggaag cggagcgccc ccagcaacca gccgcggtgg
     3421 acgaaaagac cagcgaactg gttcactttc tcttcgcgtt tcgtagcaaa caaatctccg
     3481 ctacgtacat caaccagacg ctggttccca ttctgtgcaa aaaggccggc attcccgaca
     3541 aagacgctcg cggcacgatt accagtcacc gtgcccgttc cacaatcgcc tcacagttat
     3601 ttaatgccaa ggaacccatg tcgctcttcg agctccagcg ctggctgggt cacaagtggg
     3661 caagctcgac ccaacattac ctggacgtca gtcccacaaa gctggctcag gccttccgcg
     3721 acgctggcta ttttgctcgc aacgtgcggg ccattgaagt cctgatcgat cacgaggttg
     3781 tcatagccgg gaaggcggcg cgagaaccct ggaggttcta cgatctcggt cacggctatt
     3841 gcgcctatga cttcttcgat caatgccccc accgcatggc atgcgccaag tgcagtttct
     3901 atctcccaaa aggctcctcg caagccctgc tactcgaagg gaaagcgcac ctgttgcgta
     3961 tgcggcagga gattccgctc aacgcggcag agttggctgc ggtagacgac ggagtgtccg
     4021 cattgaacaa gctcctggcc cagcttgctg acgtgccaac gcctgcgggc ccaacgcctc
     4081 gccaactccg ggattccagc ctggtccaga taacggctat tgccacggaa gggtcggtca
     4141 atgaaccagc catcggtgaa ctttaacgcg gtcaagagtg ccgtctccat ggagagggca
     4201 ctcgcacatt atggagtcat gctgcaccgc cttgatcgct gctatttacg cgggcaatgc
     4261 cctttaccga accacgcatc gaaaagcagc aaacaaagct tcattgtcaa taccgagaag
     4321 aacgcatggg cctgccattc cggctcgtgt gccgcggcgc gaggcggtcg cgtgggcggc
     4381 aacgtcctgg atttcgttgc attcatggaa agctgttcta ttcgtgatgc ggccctccgg
     4441 cttcagaact ggttcagtgt aactgccgag acggtccagc aaaacacagg cgggggatct
     4501 caacggcgta cgccgtctcc gtctttcatc caaacagagt cacccgagtc gccttcagcg
     4561 atcggcgaag aacccaatcc gcctcttccc ttcagcctca agaatgttga tatccaccac
     4621 cactatcttg gggagcggcg aatcagccca gaaactgcag agcactttgg gattggtttc
     4681 tttgcgggac aaggctacat gcaggggcgc gtcgtgatac ccattcacga tgaggatgac
     4741 cgccttgtcg cttatgcagg aagatctctt ggccagaccg aaccgagata tcgctttccg
     4801 ccccggttcc gcaaatcgct ggtcctcttc aatctgcacc gggccgtgaa tcacggcaag
     4861 accgtagttg tcgtcgaagg attcttcgat tgtctcaatg tccaccaggc gggactgcct
     4921 tgcgttgtcg ccctcatggg atgcgcgctc tctcagcacc aagagcggct tcttcagcag
     4981 cgcttccagg aggttatcct gttgctcgac ggcgataggg cgggacgcgc cgcaggagtc
     5041 gctattgctg cgcggctatg ttccaagatc tccactcggc tcgtcgagat tccctccggt
     5101 actcagcccg atcaacttgg tgccgaccag attcgatgtc tttgcattcc ggggtatttc
     5161 tgaccattgc ttatgtgtgc gccgccgccc cggcaaactg gtttcaaaag aaaaaagcag
     5221 ggagggtaac caatgggcac tggacattcc aactcatatt ccacataatc gagtccggtc
     5281 tcgactagct tgggatggca gataacgacg tcgatcccgc gctttagttg ctcgcggtac
     5341 caggcctccc gtttatgggt gggcacgctg gcccggagca cgccggcgcg aaccccctcg
     5401 gctcgcagca gcttctccag ccgtgccgtt acgtccgtat ttgttggtat agacggcaaa
     5461 cacctggcac ttgcggccgc gttcgagttg ccgcttaacc tcctcgatga gagctcgttc
     5521 cttgggatag accttgctct catccagatc ttcaggttct gcgatgatga agctctcgcg
     5581 ctgctgggtc tccgggttgt agcgattgcc gtagagcgtt cccaagccgt agggatggtc
     5641 gggatagacc agcagcgcat tgagcatggt gctcagcacg ctgcgattgc cgcgatgctc
     5701 atgcaacgtc gcggtaatct cttcttccag ttgctcgtaa gctgttttga gcgcgccttc
     5761 gagggcaacg ggaataacgt cttcccggta tgccggaagt gcatcggaga tgtcctccag
     5821 cgagacgaag gcgcatgtgt ccatcaagta cttgccgaat agcagcggtg aggctccggg
     5881 tttgcgccgg atcgtcactt ccgtcttgtt cttcctggag cacgcgttgt cttcgatctt
     5941 ggtgcgctcg atggtctcga tcacgccaaa ctcgtgggta aagcgttcct tacctgtcga
     6001 gccccaggtg aacccgtccc gcaccatgcg gctggcatcg acgcggaaga gagtgttgta
     6061 gagatcgtca gcatacccgc tcaggagagt gccggttagc ccggcgacct tcctggcca
//
LOCUS       DASRKK010000839         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1172402, whole genome shotgun sequence.
ACCESSION   DASRKK010000839 DASRKK010000000
VERSION     DASRKK010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172402"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFC15_20100"
     CDS             complement(<1..647)
                     /locus_tag="VFC15_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12518.1"
                     /translation="MNSSNGELADQAQVADLLAEAREALERANVINRAVTLCEEFLRE
                     AQFDKAFQSLDAGLLAYPGAPALVTRRCDVEGQQSAFHSAATVRTALEEAQWLLDRDR
                     PDLAARLLKQKAADLPDQPALISRLEEFEGLLPQREQNRQVQAALGRVAALEQLQQWQ
                     AALTILDEVMQYYTASEELTGAAQRFRDRLADHERKRKLARRRELIGQKMAAQSW"
     gene            complement(1043..1119)
                     /locus_tag="VFC15_20105"
     tRNA            complement(1043..1119)
                     /locus_tag="VFC15_20105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Pro,seq:cgg)
     gene            complement(1376..2236)
                     /locus_tag="VFC15_20110"
     CDS             complement(1376..2236)
                     /locus_tag="VFC15_20110"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12519.1"
                     /translation="MTLAGGETVTMQLAERGSQLSNKLWLREIRKLTDTGHQTSILTT
                     NFQAPMTTLAVSLFARWSQENFFRYMREHFGLDRLIEYGTELIPDAISVVNPAWRKLD
                     SQIRSKAGQLYRLAARFGALVLSEDPSESQVQGFQQRKGHLREETQALELEIDNLKQL
                     RKKTEHHIPVNSLPEEDQFTRLCTERKHFIDTLKMIAYRAESSMASLLREHMARGGDD
                     ARALPRQIFQTEADLTPDLAANTLTVRLHHLTQAAHDQAIEQLLADLNATQTVFPGTQ
                     LTLVFKLGSA"
     gene            complement(2292..3077)
                     /locus_tag="VFC15_20115"
                     /pseudo
     CDS             complement(2292..3077)
                     /locus_tag="VFC15_20115"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3124..3600)
                     /locus_tag="VFC15_20120"
     CDS             complement(3124..3600)
                     /locus_tag="VFC15_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12520.1"
                     /translation="MPQLQLPIFPAGVTEINSRIAVQKEAGRVFYLHGHLPVFQHEEQ
                     DVRSFRMFTSQMIAGGTVKPKEIVKTFGVPMITVKRYVKLYRDHGSKGFYEAKPRHSS
                     ASVLKAEVLEQAQHLLDAGRSVPEVAAELNVLTNTLHKAIRAGRLRGSQKKRRRGN"
     gene            4483..>4696
                     /locus_tag="VFC15_20125"
     CDS             4483..>4696
                     /locus_tag="VFC15_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12521.1"
                     /translation="MPVRLYVRMRDPEQKCIGGWRYVEDEIGSPGSSAQCASTEPSSV
                     PRVLLYPLDLTYYQCIKRVTAASHIMK"
BASE COUNT          866 a         1439 c         1389 g         1002 t
ORIGIN      
        1 ctccaggact gggcagccat cttctgcccg atcagttccc ggcggcgcgc cagcttcctc
       61 ttgcgttcgt ggtccgccag ccggtcccga aagcgctgcg ccgcgccggt caactcctcg
      121 gacgcggtgt aatactgcat aacctcatcg agaatcgtca gggccgcctg ccactgttgc
      181 agttgctcca gcgctgcgac tcgccccagc gcagcctgca cctggcggtt ttgctcccgc
      241 tgcggtagca gtccttcgaa ttcctccagg cgagagatca gcgccggttg atccggcaag
      301 tcagccgcct tttgcttcaa gaggcgggct gcgagatcgg gacggtcccg atcgagaagc
      361 cattgcgcct cctcaagcgc cgttcgaact gtggcggcgg aatggaatgc actttgctgc
      421 ccctccacgt cgcagcgccg cgtgaccagc gccggggcgc ctggatacgc aagaaggccg
      481 gcatccagag actggaaggc tttgtcgaat tgggcctccc tgaggaattc ctcgcacagc
      541 gtgacggcgc gattgattac gtttgcgcgc tccagtgctt cccgggcttc ggcgagcaga
      601 tccgccacct gggcttggtc cgcaagctcg ccgttcgacg agttcaaacc agctatcgcc
      661 tcgcgaagga gttggagcgg attgtcgcgc cctggagctg gcgcggcgat tcgcacttcg
      721 ggagcggctg tggaagtgac ctcgcgttcc cggatgcgaa cctggggaat tgcgctgatg
      781 gcagtttagt attttgtagc acgctcggga tatcatcgtc caaagaatgc ctggattcat
      841 cgggtgttat cgacaagttt ctggaagtct ggaatgtgat gattagggaa ttttaaatgt
      901 tcaaagattg tgaataaaac gaggaagcga atgtgtgtcg gacgggagta ctggaagaaa
      961 gcggcgtttt tgatctgcgg atcagttgat agatccgcct caaggccagg tttggacaac
     1021 cgggaggagt tgttgatttg cttggtcggg gcggaaggat tcgaaccttc gacctcctgg
     1081 tcccgaacca ggcgctctac caggctgagc cacgccccga caaggccgtt cctttcgtac
     1141 tatagcacgt ccggctggtt tgggcaggtt tgccgaccag acgctctgct tgaaaaccat
     1201 cacgacccag gaaaacacac ccaaatattc ggtgtcaagc catacccctt ggaatacgag
     1261 atctcgtagc ggctgggcaa tggtggaatc atggagacaa tcgacaaacg agcgatcctg
     1321 aaggaccaca tggcacgggt ttcgcgctac ttcagacctc ctgacctggg tgaaatcagg
     1381 ctgagccgag tttgaagacc agcgtgagct gggtgccggg aaacaccgtc tgcgtggcat
     1441 tgaggtcggc gagcagttgt tcgatagcct gatcgtgagc tgcttgcgta agatggtgga
     1501 gcctgacggt cagtgtgttg gcggccaagt cgggcgtcag atccgcttcg gtctggaaga
     1561 tctggcgcgg taacgcgcgg gcatcgtcgc cgccgcgggc catatgctcg cgcagcagcg
     1621 acgccatgct ggattcggcg cgataggcga tcatcttgag agtatcgatg aagtgctttc
     1681 gctcggtgca caggcgagtg aactggtctt cctccggcag tgaattcacc ggaatgtgat
     1741 gctcggtttt cttccgtagt tgtttgagat tgtcgatctc caattccaga gcttgagtct
     1801 cttcccgcag gtgtcctttg cgctgctgaa aaccctggac ctgagactcg ctgggatctt
     1861 cggataagac cagggcaccg aaccgagcag ccagccgata gagttggcca gccttggagc
     1921 ggatctggct gtcgagcttg cgccacgccg gattgaccac cgaaatcgca tcaggaatga
     1981 gctcggttcc gtactcgatc aggcggtcca ggccaaagtg ttctcgcatg taacggaaga
     2041 agttttcctg agaccatcgg gcgaatagcg acaccgccag cgtcgtcatc ggcgcctgaa
     2101 agttcgtcgt cagaatcgag gtctgatgcc cagtgtcggt gagcttgcga atctcgcgca
     2161 accaaagctt gttggacaac tgcgagccgc gctccgccag ttgcatggtc acggtttcgc
     2221 ctccggccag tgtcacgctg tacgtggcga actcttccct ccgccaatcg tccgcgcgga
     2281 aacttgtgat aactcagaat ggcaatccgc ttctgccgca tctgctcgaa caactccggg
     2341 ctgtagcctt cccggccgaa caccatggtg aaccagtggg cgcgtggatc ttcggctagt
     2401 cgctgctcct gctccggcgt cttggccacg ttcgcctcta accatggaat tacatcccct
     2461 ttgagcgtgg cgatcagccc ggggtccact tccttgttca cgtataggaa cggttggccg
     2521 tccatggcat tgatccagta atcggtcgtg gcccgcagac acaaccgctc gcgggccaca
     2581 tagtggcgcg gcagcgcggt ctggtctccg tgatacacgc gcacgtggcc gtcgcagtag
     2641 aaatagaggt ccgtggcgtt ttgccgggca atccattctt gggccaaggc ggcgttccat
     2701 cgcatcgcca gccccatgtc ctggcacagc agcttcaact tcgcacgcag cgtccgcact
     2761 tcggggattc gatccagccc cagcagtttg ccccattcgc ccggcgcctg gtagcgcagt
     2821 tgttccagcg atcggatgcg cgccagcgcc atcaacccca ggagcaggaa gatgctctcg
     2881 atgccataga atcccttggg cagttggtag aactcaggcg tataacgcag cagccccgac
     2941 gccaacaggg ccggcaacgc cagcagcact ccaccttgcg ctacgtcgca agtggcttga
     3001 aattcgatcg gcgccgactc cacttccccc atggccgccg ccacgcgttc cagggaacgc
     3061 gtcgcgccgt tgcccatcgg cgcctggctg tcggtctggc tgcgttcgct cttgttggtt
     3121 acttcagttt ccgcgcctgc gttttttttg agaacctcgc aaacggcccg cgcggatcgc
     3181 cttgtgcaaa gtgttcgtca acacgttcag ttccgccgcc acttccggca cgctccgtcc
     3241 cgcatcgagc aagtgctgcg cttgctccag cacctcggct ttcagcaccg acgccgaact
     3301 atgccgcggc ttcgcctcgt agaacccctt ggagccgtgg tccctgtaca acttcacata
     3361 ccgcttcacc gtgatcatcg gcacgccaaa cgtcttgacg atctctttgg gtttcaccgt
     3421 cccgcctgca atcatctggc tggtgaacat gcggaaactc cgcacgtcct gctcctcgtg
     3481 ctggaacacc ggcaagtggc cgtggagata aaacaccctg cccgcctctt tttgcaccgc
     3541 gatccggctg ttgatctcgg tcaccccggc aggaaaaatc ggcagttgaa gctgcggcat
     3601 tcttctctct taccccaatt gcagtaccct ctgcaaatac atttcccgga tcattctgtt
     3661 ttcaccgagt ggcagaaatc actggggcga gatcaggaga tctgaacttg cagcgggggc
     3721 aacggtccgg ggttggcgtc aggcctaaag attgtttagg acccatgtcg tcgttgcgcg
     3781 gcgtgcgtcg ccgggtacga cgcgcagcgc tggggccttt ccggtggcct ggcggggttg
     3841 tccctgctgc gcgggccagc ctctccagca cacacgagaa ggccccgcgc cccggcaaca
     3901 aaagcaacgg gtctccgcac ccctctaaaa taaggaaaaa acacagaagg agtctgtgca
     3961 gacccgttgc cgccgttgcc ggtgctttgc tgctgctctc ccgtgtggtg ttgtcttccc
     4021 ccgtgcctat gtttgccgct ggttccgggc caggcgagcg ccggtgcccg tgccccccgc
     4081 cgcctcgctt ctggtgttgc ccccaactgt gtgttgcaga ggcacacgag aaggaccggc
     4141 agtggcgtgc gtcccaaccg gcctggggag actttttctg attcgccgga gactcgccct
     4201 tttcgaatcg gcaccctctg aggaccttca gcaggcgggg tcgccgtgat gtcagggacc
     4261 attgacatca catttgcagc cggagctacg gcccgaccac cagtcctgcc agggccaagt
     4321 tgccactcgc ggtggcattg atgagctggt gttaacgggt tcgattcccg ttagcgctac
     4381 caattcctca tagagttacg ggcaacgccc catgacgccc catgacgcgc aacgcgccag
     4441 ccgaacgctt tctcccttca ctgagttcgc gaggtctcaa atgtgccggt gcggctctac
     4501 gtgcggatgc gcgatcccga gcagaaatgc atcggcggtt ggcgttacgt cgaagacgag
     4561 attggatctc ctgggtcgag cgcacagtgt gcatcgaccg agccatcgag cgtcccgcgc
     4621 gttctgctgt atccgctgga cctgacgtat taccagtgca tcaagcgcgt gaccgcggcg
     4681 tcccacatca tgaagc
//
LOCUS       DASRKK010000840         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_976644, whole genome shotgun sequence.
ACCESSION   DASRKK010000840 DASRKK010000000
VERSION     DASRKK010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976644"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1551)
                     /locus_tag="VFC15_20130"
     CDS             complement(115..1551)
                     /locus_tag="VFC15_20130"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZM12522.1"
                     /translation="MCTENSVVKSRAQLWDWGQAHLNELVDYTLELQQEVSRLRDSAA
                     QNSSNSSRPPSTDRPETPQPKSLRRKSGRPSGGQPGHPGRTLQSSPNPQHLQIHPLRE
                     CECGEDLSQEPALDFERRQVFDLTTLRLECTEHRAEIKECPNCGRTSRAPFPADLKAP
                     VQYGKNFRALLAYLYDAQAGASLRIRQMGQEMFGFAISEATLQKARQEQYDALEPFEN
                     RLMEILPQEALLHADETSVPINKANHWLHVLCTPLLTFFSVQPNRSKEAIQTIGIIPR
                     FVGWLMHDFLSSYLSFENCLHTFCKGHLLRELVFLFEQHQQPWAKDLYDLFLAMRLCV
                     KDRKARDAPLTQAELGRWYTQYRKILRAGRQANPIIPEQTGKKRPKQSKEQNLLDRLE
                     GCEDCILAFLWNFKLPFTNNQAERDFRMMKVRVKISGCFRTLEGARRHARIRSYISTL
                     RKHGLPVLEYLRHALDGHPFLPVGSQST"
     gene            complement(1526..2413)
                     /locus_tag="VFC15_20135"
     CDS             complement(1526..2413)
                     /locus_tag="VFC15_20135"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HZM12523.1"
                     /translation="MLEAEIVQGRKISDVEISEIKLLMAANPLWSRYRLSRVLAQRWQ
                     WYSVSGQLKDMAARTLLQKLHQRDLIALPEPRRGQVRRAPQLSLEPFDAVVPASVVGD
                     LSSLQPLEIQVVGPKQPDYHRFRRYLAQHHYLSYGGPIGENLGYLIKSCAGVELACLL
                     FGAAAWQCAGRDQWIGWSAEQRAAGLQFIANNSRFLILPWVRVRHLASHILARIARRI
                     AADWQKRYHHGIHLLETFVQPDRFRGTCYQAANWIRVGQTTGRTRQSRRQRDNLVHAP
                     RKDIYLYPLVIDVHRELCR"
     gene            complement(2513..>2704)
                     /locus_tag="VFC15_20140"
     CDS             complement(2513..>2704)
                     /locus_tag="VFC15_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12524.1"
                     /translation="VKGGISALFACIPIIHVVLGLVFIIAPQVFGHGNNQPPAFLGLL
                     LVMLGGFLILFGWTFALLA"
BASE COUNT          599 a          728 c          825 g          552 t
ORIGIN      
        1 gctttacaac aacaatggaa atgcgttgac tcggagttat ggatggcaag ctaacttcgt
       61 gcccatcatc aagagcgatc tgaggcagcg aagagatcct gagaaggtaa atactcaggt
      121 ggattgtgat cccaccggca ggaaggggtg accgtcgagt gcgtggcgga ggtattcgag
      181 cactggcagg ccatgcttgc gcaaggtcga gatataactg cggatgcgag cgtgacgacg
      241 ggcgccttcc aatgtgcgaa aacagccgga aatcttgacc cgcactttca tcatgcggaa
      301 gtcgcgctcg gcttggttgt tggtaaaggg caatttgaag ttccacagga atgccagaat
      361 gcaatcttcg cagccctcca accgatccaa taagttctgt tctttggatt gcttagggcg
      421 cttttttccc gtttgctcgg gaatgatcgg gttggcttgg cggccagcgc gtaatatctt
      481 cctgtattgc gtgtaccaac ggcccaactc cgcttgcgtc aagggcgcat cccgggcctt
      541 gcgatccttg acgcacaggc gcatcgccag gaacaggtcg taaaggtctt tggcccacgg
      601 ttgctggtgt tgttcaaaga ggaacactaa ctcgcgcagc aggtggcctt tgcagaaggt
      661 gtggaggcag ttctcaaaac tcaggtagct ggaaaggaag tcatgcatca accagcccac
      721 gaatcgggga atgatgccga tggtttggat cgcctctttg ctgcgattgg gttggacact
      781 gaagaaggtg agcaaaggcg tgcacagcac gtgcagccaa tggttggcct tgttgatggg
      841 cacgctcgtc tcgtcggcgt ggaggagcgc ttcttggggc aagatttcca tcaaccggtt
      901 ttcaaagggc tccagggcgt cgtattgctc ttggcgagcc ttttgcaggg tggcttcact
      961 aatggcaaag ccgaacatct cttggcccat ttgacggatg cggaggctgg cgcccgcttg
     1021 ggcgtcgtag aggtaagcca gcaaggcacg aaagtttttc ccatattgga caggggcctt
     1081 gaggtcggcc ggaaagggcg cccgcgaggt tcgcccgcag ttggggcact ctttgatttc
     1141 ggcccgatgc tccgtgcatt ccaagcggag cgtggttaag tcgaagactt ggcgacgttc
     1201 aaaatccagc gccggttcct gggaaagatc ctcgccgcat tcgcactcgc gcagaggatg
     1261 aatctggagg tgctgggggt tagggctgga ttgaagggtg cgaccggggt ggccaggttg
     1321 accgccggag gggcgtccgg actttcggcg cagactcttg ggctggggcg tttccggccg
     1381 atccgtggaa ggcgggcgcg aactgttgct gctattctga gcggcggagt cgcgcagacg
     1441 actaacctct tgctgcaact ccaaggtgta gtccaccagt tcgttcaggt gggcttggcc
     1501 ccaatcccag agctgcgccc ggctcttaac gacagagttc tcggtgcaca tcgatcacta
     1561 aaggatagag gtaaatgtcc ttgcggggag catgaaccaa gttgtcccgc tggcgccgac
     1621 tctggcgcgt ccggccggtc gtttgaccca cccggatcca gttggccgcc tggtaacaag
     1681 tgccccggaa tcggtccggt tgcacgaagg tttccagcag gtgaatgccg tggtggtagc
     1741 gcttttgcca gtcggccgcg atgcgccggg caattcgcgc aagaatatgg ctggccagat
     1801 gccgaacgcg gacccagggc aagatcagaa aacggctgtt gttggcgatg aactgcaagc
     1861 ccgcagcgcg ttgctcagcc gaccaaccga tccactggtc tcggccagcg cactgccagg
     1921 cggccgcgcc aaagagcagg caggccaact cgaccccggc gcaactctta atcaaatagc
     1981 cgagattctc gccaatggga ccgccatagc tgagatagtg atgctgggcc aaataccgcc
     2041 ggaaccgatg atagtcaggc tgcttggggc cgaccacttg gatctccagg ggctggagtg
     2101 aagacaaatc gccaacgacc gatgcgggaa cgactgcatc aaaaggttcg agactcaact
     2161 gtggcgctcg ccgaacctgc cctcgtcggg gttccggtag cgcaatgagg tctcgctggt
     2221 gaagcttctg cagcagcgtt cgagcggcca tgtccttgag ttggcccgaa acagaatacc
     2281 actgccatct ctgggccaaa acacgagaca ggcgatagcg gctccaaagc ggattagccg
     2341 ccatcaacaa cttaatctct gagatctcga cgtcgctgat ttttcggcct tgaacgattt
     2401 cggcttccag cacgcggcca aactaccaag cagattccgc tgagtcccgt acaaagtgaa
     2461 attacttttg cctcaaatcc caccgccagt gctcatctgg ccccctgagt aattacgcta
     2521 aaagggcaaa ggtccaaccg aagagaatga gaaaaccgcc cagcatcacc aacaacaagc
     2581 caagaaatgc cggaggttga ttattgccgt gtccgaatac ctgtggagca atgatgaaga
     2641 ccagcccaag gaccacgtga atgatgggga tgcaggcaaa cagagccgat atgccaccct
     2701 tgac
//
LOCUS       DASRKK010000841         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_1269934, whole genome shotgun sequence.
ACCESSION   DASRKK010000841 DASRKK010000000
VERSION     DASRKK010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269934"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFC15_20145"
     CDS             <1..648
                     /locus_tag="VFC15_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM12525.1"
                     /translation="RFLYLQNITSLRLETSVPPVGGWRLATLPPPAMMATDVQVLLDS
                     CDRNTAVGIRDFAIMMLVARLGLRSIEVARLELGDMDWRSGELVVRGKARRLDRLPLP
                     AEVGEALVAYLSCGRNPVDAVHVFLTCRAPRGPIRADLVGDVVERACKRAGSTKVGPH
                     RLRHALASELLRNGAGLVAISQVLRHQDLATTALYAKVDLNMLRHVAQPWPGTVR"
     gene            645..1577
                     /locus_tag="VFC15_20150"
     CDS             645..1577
                     /locus_tag="VFC15_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM12526.1"
                     /translation="MSVLEQDLVDYLKLRRSLGHQLADAGRLLPSLVAYLDTHSLSTV
                     TIQAALEWAQQSVTGQATSRGSRRMTAARGFARYLAGIYASTEVPPLGLMPHRQRWRR
                     PFIYTPADIESLMSQARCSITSPLRAATYETLIGLLAVSGLRIGEAIKLDRDDVDWAG
                     GVLLIRESKFNKTRLVPLHDSAITALAEHAELRDTLQPRPKEPSFFVSLTAKRLCYAA
                     VSPTFRRLVDNAGIGVGAPSAPRIHDLRHSLAVHTLLSWYRSDEDVQAKIPSLSTYLG
                     HREPSSTYWYLSAVPELLALAAARQDTNWLAVRS"
     gene            1574..2563
                     /locus_tag="VFC15_20155"
     CDS             1574..2563
                     /locus_tag="VFC15_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM12527.1"
                     /translation="MTLVAPTLQMFFTDRLVQQRQVSPRTIAAYRDTLRLLLGFVHQK
                     TRKLPAQLDWDDLDSTIVSEFLNYLENERHNSIRTRNVRLTAIRSLFSYAALRHPEHA
                     LLIQRVLAIPPKRFDKRIVTYLSPPEIDALIAAPDQTRWEGRRDRALMLLAVQTGLRV
                     SELTGLNCRDVTLGVGANVCCEGKGRKQRAVPLTSPVQTLLRAWLAERAGLPGDPLFP
                     TRTGRRLSRDAVALRVSTHAAVAAKACPTLLGKSIHPHVLRHSCAMSLLQAGVDTSVI
                     ALWLGHAGVRSTDAYVHADISIKEKALALTTPATARPGRYHPPDKVLAFLESL"
     gene            2694..2855
                     /locus_tag="VFC15_20160"
                     /pseudo
     CDS             2694..2855
                     /locus_tag="VFC15_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          565 a          900 c          802 g          588 t
ORIGIN      
        1 cgttttctct acctgcagaa catcacgtct ctgcggctgg aaacttcagt gcctccagtg
       61 ggtggatgga ggttagcgac tttgccacca ccggcgatga tggccacgga cgtgcaagtg
      121 ttgctcgata gttgcgaccg caacactgcg gtgggtatcc gtgactttgc gatcatgatg
      181 ttggtggcgc ggctgggttt acgttcgatc gaggtcgcgc ggctggagct gggcgacatg
      241 gattggcgct caggtgaact tgttgtccgc ggcaaggcac gccgtctgga ccggttgccg
      301 ttaccggccg aggtcggcga agctttggtc gcctatctgt cgtgtggtcg taaccctgtt
      361 gatgccgttc atgtgttctt aacctgccgg gcgcctcgcg gaccgatccg ggccgatctg
      421 gtcggcgatg tcgtcgagcg ggcctgcaag cgtgccggat cgacgaaggt tggcccacat
      481 cgtttgcggc atgctctggc cagcgaactg ctgcgtaatg gtgcaggttt ggtcgctatc
      541 agtcaggttc ttcgccacca ggatttggcc actactgctc tttacgccaa ggtcgatctc
      601 aacatgctgc gccacgttgc tcagccctgg cccggaaccg tccgatgagc gtcttggagc
      661 aggatctggt ggattacctg aaactgcgtc gttcactggg acatcagctg gccgacgccg
      721 gacggctgct gccaagtctc gttgcctacc ttgatacaca ctcgctatcg acggtgacga
      781 tccaggcggc acttgaatgg gcgcaacaat cagtaaccgg gcaggcgacg tcgaggggat
      841 ctcggcggat gacggccgcc cgtggtttcg cacgctattt ggccggaatc tacgcaagca
      901 cggaggttcc accgcttggt ttgatgccgc atcggcaacg ctggcgccgc ccatttatct
      961 acaccccagc cgatatcgaa tcgttgatga gccaagcacg ctgctcgatc acatccccgc
     1021 tgcgggccgc cacctatgag accttgatcg ggttgttggc agtcagcggg ttgcgtatag
     1081 gcgaggccat caaactcgac cgcgatgatg tcgactgggc cggcggcgtg ttgttgatac
     1141 gtgagtccaa gttcaacaaa acacgactgg ttccgctcca tgacagtgcc ataacagccc
     1201 ttgctgagca tgctgagtta cgcgacacat tgcaaccacg gccgaaagaa ccaagcttct
     1261 tcgtgtccct gacagccaag cgactgtgct atgcagcagt gtcaccaaca ttccggcgac
     1321 tcgtcgacaa cgcgggcatc ggggtcggcg caccctcagc gccaagaatt catgacttga
     1381 gacattcgct cgcggtccat accctgctca gttggtatcg cagcgatgag gacgtgcagg
     1441 cgaaaatccc gtcgctatct acctatcttg gccaccgcga accttcctct acctactggt
     1501 atctgtcggc tgtcccggaa ttgcttgctt tggcggcagc ccgccaggac accaactggt
     1561 tggcggtgcg ctcatgacat tggtcgcgcc gactctgcag atgttcttca ccgaccggct
     1621 cgttcagcaa cgtcaagtaa gcccccgcac gatcgcggct taccgagaca cgctgcgcct
     1681 actgctcgga ttcgtgcacc agaagacccg caaactgcct gcccaactcg actgggacga
     1741 ccttgacagc accatagtct ccgaatttct caactattta gaaaacgagc gacacaacag
     1801 catccggacc cggaacgttc gactaactgc gatccgctcg ctgttctcct acgccgcttt
     1861 gcgccacccc gagcatgcac tgctcatcca gcgcgttcta gcgatcccgc cgaaacggtt
     1921 cgacaaacgc atcgtgacat acctgagccc acccgaaatc gatgcactta tcgctgcacc
     1981 agaccaaacc cgctgggaag gtcgccggga cagggctctc atgttactgg cggtccagac
     2041 cggcctgcgg gtctccgaac ttaccggtct gaactgccgc gacgtcactc tcggtgtcgg
     2101 cgcgaacgtc tgttgcgagg gcaagggacg caaacaacgc gctgtcccgc tcaccagccc
     2161 cgttcagact ttgctgcgag cctggttggc cgaacgagcc gggctacctg gagacccact
     2221 gtttccaacc cgcactggcc ggcgcctcag tcgcgacgct gtcgcgttac gggtcagcac
     2281 gcatgctgca gtcgctgcga aagcatgtcc aacgctgctg ggcaagtcaa ttcatccaca
     2341 tgtgctccgc catagttgtg ccatgtcgct gctccaagca ggcgtcgaca cgtcagtaat
     2401 cgcgctttgg ctcggtcatg ctggagtccg atccacagac gcctacgtcc acgccgatat
     2461 cagcatcaaa gagaaagccc ttgccctcac aacaccagca acagcgcgac ccggccgcta
     2521 ccatccgccg gacaaggtcc tcgcgttcct ggagagcctg taactatgcc gataccagaa
     2581 ccgaccacag gacgaagaaa ccctgcacca tcaagcgatt gagcgccgct cgtcgccttc
     2641 ctcggcatag tcactggctc ggcatagatc gatctatgcg gatatccgca tagatcgatg
     2701 aattcggata tctgcccatc gatactgagg gggccaggct cctgttccaa gtcatcgccg
     2761 acagttacga gaaacgcagc ctgatcatca ccacgaacct cgagttctcc cgatggggaa
     2821 ccgtgttcgg tgacgacaac atggcagccg ccgtc
//
LOCUS       DASRKK010000842         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_586146, whole genome shotgun sequence.
ACCESSION   DASRKK010000842 DASRKK010000000
VERSION     DASRKK010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586146"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /gene="nusB"
                     /locus_tag="VFC15_20165"
     CDS             <1..467
                     /gene="nusB"
                     /locus_tag="VFC15_20165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZM12528.1"
                     /translation="RGQRSGRKAMTKAGTRRKSRELALQMLFQMEMGKQNPDQVRQSF
                     WSERQDLDDKVRGFADDLFGIAAERAEEIDQLIERNAEHWRMDRMAAVDRNLLRAGVA
                     EFLGFPETPRAVVINEALEIARRFSTPESVHFINGVLDSVARELGEGKKKTE"
     gene            complement(867..>2181)
                     /locus_tag="VFC15_20170"
     CDS             complement(867..>2181)
                     /locus_tag="VFC15_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12529.1"
                     /translation="QALTADVLDTNGQSIKPPLTWGSSAIASATVTNTGTATNNPGTI
                     TAVAPGTAYITASCSNPSCNISLPPQYSLNLVTATVTGSTSTTVYAASTNSLMLVPIS
                     TSTNSAGTAITLPFLPNSMVADPAGATLYLGSSSGLMGVNLSTSAVSSLAGVNGTIEA
                     ISPDGKYLLLSDSVANSIHYFGVSTLSVLFTQPGDTTNSSAYTPDSQLNEWLNGNQLG
                     VGLPTAFLSATTLAYTANALDISGQGGLTYISSSSAQQIHVLATCNQAEVQVPPLPVN
                     SPTLIKAIPNGTGAVAADSPAIDVVTTGAVPAGCPPAAPNSVHSFDMGAGPFNTRQIL
                     LSPDSSNAWIISDNLLGLLNFNLVNSTPAPIIPFTGGVTACIVGVPACSGGITLDGAQ
                     VYVGASDGTVHRIDVASNSDAQQIAVGLKDGNGNPVAPNLVTVLP"
BASE COUNT          450 a          574 c          701 g          456 t
ORIGIN      
        1 cacgtggcca gcgcagcgga cggaaagcga tgaccaaggc cggcactcgc aggaagtcgc
       61 gcgaattggc gctgcagatg ttgtttcaaa tggaaatggg aaagcagaat cccgatcagg
      121 tgcggcaatc gttctggagt gaacgccagg acctcgatga caaggtacgg ggctttgccg
      181 acgacctgtt cggtatcgct gccgaacgcg cagaggagat cgaccagttg attgagcgca
      241 atgcggagca ttggcggatg gaccgcatgg cagccgtgga tcgcaatctt ctgcgcgctg
      301 gggtggctga gtttttagga ttcccggaaa cgccgcgtgc ggtcgtgatc aacgaggccc
      361 tggagattgc gcggcgtttt tccacgccgg agtcagtgca cttcatcaac ggcgtactcg
      421 acagcgtggc ccgcgaactg ggagaaggca agaagaagac agaatgaaaa gcaacgttcg
      481 ggtagtgcgg accttcaagt ccgcattcaa gccttttgct taatgattcc agagtcggct
      541 tcacaggctg cggaaaaacc ctgtgcaaaa ccgtggagtg tacttcttgg tggaagcccc
      601 cgcatttatg cgggggaagg agcgcttcat cgctccggaa aagttcggct ttgagcacgc
      661 gctttagcgc cggggatcga gaatcccggg gctaaagccc atttcaaaat cgcccccttt
      721 tacgctggac taaagtcgag atcccgtgct gaagcagggg gcttctacca aaaaccttcc
      781 ctcgagtttt gtcgcagcct taagctaggt accgatctca aggcccatcc tacgcagcca
      841 tccccggcga ctttccagca gcttgcttag ggcagcacgg tcaccaggtt gggtgcgact
      901 ggattgccgt taccgtcctt aagcccaacg gcgatttgct gtgcgtcgct gttgctggcg
      961 acatcgatgc ggtgcacggt gccgtcgctg gcgccaacgt aaacctgggc gccatcgagg
     1021 gtgatgccgc cgctacaagc tgggactccg acgatacaag ctgtgactcc accggtaaag
     1081 ggaataatgg gggctggagt tgagttcaca agattgaagt tcaacaagcc tagcaggttg
     1141 tcgctgatga tccatgcatt cgaactgtca gggctcagta agatctgccg ggtgttgaat
     1201 ggtcctgcgc ccatgtcaaa actgtggacc gagttcgggg cggctggcgg acagcccgcc
     1261 ggaactgccc cggtcgtaac tacatcgatt gcgggcgaat cggcagccac cgcgcctgtt
     1321 ccgttaggaa tcgccttaat caaggtcgga ctgttcacgg gaaggggtgg aacctgcact
     1381 tcggcctgat tgcaggtggc gagcacatga atctgctggg cactgctgct gctgatatag
     1441 gtcagtccgc cttggccact gatgtcgagg gcgttggcgg tataggcgag cgtcgtcgcg
     1501 ctaaggaatg ccgttggaag tccgacgcca agctggttcc cattcagcca ctcgtttagc
     1561 tggctatcgg gagtgtaagc gctcgagttg gtcgtgtcac caggctgtgt gaacaggacg
     1621 ctcagggtgc tgacgccaaa gtagtgaatg ctattcgcca cgctgtccga cagcagcagg
     1681 tatttcccat ccggcgaaat cgcctcaatc gtcccgttca ctcctgcaag gctgctgacc
     1741 gcgctggtac tcaagttaac gcccatcaaa ccggagctgg atccgagata aagcgtagcg
     1801 ccggcagggt cagcgaccat cgagttcggc aggaagggaa gcgtaattgc cgtgccggcg
     1861 gagttggtcg aagtgctgat gggaaccagc atgagggaat tcgtgctggc agcatagacc
     1921 gtggttgacg tcgagccggt tacggttgcg gttactaggt tcaaactgta ctgagggggc
     1981 agagaaatat tgcagcttgg attgctgcag cttgcggtga tgtaagccgt tccgggcgcc
     2041 accgccgtga tcgttccggg gttgttggtg gccgttcccg tgttggtgac cgtggcactt
     2101 gcgatggccg aggaacccca ggtgagcggc ggtttaatgg attgaccgtt ggtgtcgagc
     2161 acgtcggccg tgagcgcctg c
//
LOCUS       DASRKK010000843         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_586149, whole genome shotgun sequence.
ACCESSION   DASRKK010000843 DASRKK010000000
VERSION     DASRKK010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586149"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFC15_20175"
     CDS             complement(<1..210)
                     /locus_tag="VFC15_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12530.1"
                     /translation="MPLIWLRVAVVCYGVGLLYSLLLLSKRGNVLAKVVEPAVGLGLI
                     FHFVSLVESTVLIGHLSLASIHYSES"
     gene            complement(369..1082)
                     /locus_tag="VFC15_20180"
     CDS             complement(369..1082)
                     /locus_tag="VFC15_20180"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZM12531.1"
                     /translation="MDRALRAEKAQVPRVGDFLMFTQMQWPGRKRWIRALLASFAVHG
                     LFLFLLIHRAGAIFVIPADVDLGIKGSSGSMSIVYLAPVGPEETQSSPDEQRPALRAA
                     LAPKPKLHKPDTKVRQTEQPTREDAPEQTARGGSPYGRVPGSPITGEEVVPALPEVFP
                     DPPVSRSDIPAGVQGDVIVEVTIDAEGNVVQTKLLQGIGYGIEQKVLSVLQRWHFRPA
                     SKDGVTIASQHIVYFHYPS"
     gene            complement(1146..1508)
                     /locus_tag="VFC15_20185"
     CDS             complement(1146..1508)
                     /locus_tag="VFC15_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12532.1"
                     /translation="MTIRRVKTYTGQTGYVYQYYFVGSRPALDNDPEAPSTEFIFDVT
                     SDRKTIFAVSIFLHPEALFAWAASRGRTLSQSEQYAAVKLRLMQAFDQIPDMLNHGRR
                     LRLEVHELPVLLDSIGVE"
     gene            complement(1552..>2271)
                     /locus_tag="VFC15_20190"
     CDS             complement(1552..>2271)
                     /locus_tag="VFC15_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12533.1"
                     /translation="IVENARHDRHLWNADDREIEEYLALCGQRIQDLKGDVRFKYISV
                     FKNFGTHSGQEFDHPTSEIVATTFVPRRVLYELRAARQHYQVKERCVFCDILRQEQEQ
                     KLRVIEVRGDYIACCPYAPRVPYETWVAPHTHEAYFERMALRSGAMRDLAALLRRTLY
                     RIRTIADGFHMVLHTSPNTMHRSETLGFWKTIEDDYHWHIEILPILGAKAKSYTFKEV
                     YYSPVSSETAAAILRERPTEP"
BASE COUNT          482 a          651 c          692 g          446 t
ORIGIN      
        1 ggattccgag tagtggatcg aagccagcga aagatgaccg atcaggacgg tggactcgac
       61 cagagaaacg aagtggaaga taagccccag ccccacggcc ggctctacca ccttggccag
      121 aacgttgcct cgcttgctca gcaggagcag cgaatagagt agccccacgc cataacaaac
      181 caccgcgact cggagccaga ttaacggcat aaaccccacg aatgtaatgc ttaagctcga
      241 ttgtttcttc taaggttaag tataacgggg tgccgggtag ggaagagtgt ggttatcact
      301 tccggctgtg atttgcgtca caagggaacc ggctccccgc atgaagcatg gacgcagaag
      361 tggcacccct agctgggata gtggaagtac acgatgtgct gcgaggcgat ggtcacgcca
      421 tccttcgagg cgggccggaa gtgccatcgt tgcagcacgg aaagcacctt ttgctcgata
      481 ccgtatccga tgccttgcag cagcttggtt tggaccacgt ttccttctgc gtcaatcgtg
      541 acctcgacaa tcacgtcgcc ttgaacgccc gccggtatgt ccgagcgcga taccggagga
      601 tcgggaaaga cctcaggcag cgccggcacg acttcctctc cggtaatcgg cgaaccgggc
      661 acacgtccgt aaggagaacc gccgcgcgcg gtttgctcag gcgcatcctc ccgcgtgggc
      721 tgttctgttt gccgaacctt cgtatcaggc ttgtgcagct tcggcttggg cgccagcgca
      781 gcacgaagcg ccggccgctg ctcgtccggc gatgactggg tctcctctgg tcctacaggt
      841 gcgagataaa cgatagacat cgatccggag gatcccttaa ttcccagatc aacgtcagca
      901 gggattacga aaatggcacc agcacgatgg atgagcagaa acagaaacag gccgtgaact
      961 gcgaaggatg cgagcagagc acggatccat cgcttgcgtc cgggccactg catctgcgtg
     1021 aacatcagga agtcccccac ccttggaacc tgcgccttct ccgcgcgaag ggctcggtcc
     1081 atcccatctt tactgctcgc ttggacgcaa gcaatcagaa tggcttagct gcaacaattg
     1141 taccgctatt ccactccaat cgagtccaac aatacgggca gttcatggac ctcgaggcgc
     1201 aagcggcgtc cgtggttgag catgtcggga atctgatcga aggcttgcat caggcgcagc
     1261 ttcactgcgg cgtactgttc cgactgacta agcgtgcggc cccggctcgc cgcccatgcg
     1321 aacagcgcct cgggatgcag aaagatactg accgcgaaga ttgttttccg gtccgacgtc
     1381 acgtcgaaga tgaactcggt tgagggagct tctggatcgt tgtcaagcgc gggccggctg
     1441 ccgacgaaat agtactggta aacgtagccg gtctgcccgg tataggtttt gacacggcgg
     1501 atggtcataa aacgcgttgc tacccgaggg aatattgtag tccggtcgca gctacggctc
     1561 cgtgggacgc tcgcgcagga ttgccgccgc cgtctccgaa ctgacgggcg agtagtacac
     1621 ctctttgaag gtgtaggact tcgccttggc gccgaggatg ggcagaatct cgatgtgcca
     1681 gtggtagtcg tcctcgatgg tcttccaaaa cccgagcgtt tccgagcggt gcatggtgtt
     1741 gggagaggta tgcagcacca tgtggaaacc gtcggcgatg gtgcggatgc gataaagcgt
     1801 acgccgcagc agcgccgcca ggtcgcgcat ggcgccggac ctcagcgcca tgcgctcgaa
     1861 ataagcttcg tgggtgtggg gagcgaccca ggtctcatac ggcacacggg gagcgtaagg
     1921 acagcaggca atgtagtcgc cgcggacttc gatgacgcgc agcttctgtt cctgctcctg
     1981 cctcagaatg tcgcagaaga cgcagcgctc cttcacctgg taatgttgcc gcgccgcgcg
     2041 caactcgtac aggacacgtc gcgggacaaa cgtggtagcc acgatctccg aggtggggtg
     2101 gtcaaactcc tgccccgagt gggtgccgaa attcttgaag acgctgatgt atttgaagcg
     2161 gacatcgccc ttgaggtcct ggatacgctg gccgcacagc gccaggtact cctcgatttc
     2221 gcggtcgtcg gcgttccata aatgccggtc gtggcgggcg ttttccacaa t
//
LOCUS       DASRKK010000844         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U10160 k141_879464, whole genome shotgun sequence.
ACCESSION   DASRKK010000844 DASRKK010000000
VERSION     DASRKK010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963688
            Sequence Read Archive: SRR5664279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:16:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,013
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 13 of 52
            Pseudo Genes (incomplete)         :: 41 of 52
            Pseudo Genes (internal stop)      :: 6 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879464"
                     /isolate="SMAG_U10160"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFC15_20195"
     CDS             <1..605
                     /locus_tag="VFC15_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM12534.1"
                     /translation="DDTSTLFIDYCFLRCSRTRLNDPSQFIKSAWIRPEDLAVYEAMG
                     YTTFKLLERGIPSAELLRRVKAYSERRFDGNLAELLLSYGFKQPVRKESLWSLRHFWK
                     PQQVNPRRLKPLLDLARLQGWLSPLPECPIRVEARKIPENFLDGFRERDCASLDCQTC
                     GYCERIAAQAVSIASEYRSEVLRKYAEMDDTMVAGALWGV"
     gene            922..1335
                     /locus_tag="VFC15_20200"
     CDS             922..1335
                     /locus_tag="VFC15_20200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HZM12535.1"
                     /translation="MKTLFTAEAISKGGRSGTIQTPDRLLDVTLGNPMEKGIEKRGPN
                     PELLFAGAYSACYHGALGNAAKKLGTPVRDSTVRALVSLIEEDQGGYRLAVELHAQLP
                     GLDRAQAQRLMDEAHRTCPYSRALRGDTSVKLVVD"
     gene            1332..3080
                     /locus_tag="VFC15_20205"
     CDS             1332..3080
                     /locus_tag="VFC15_20205"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZM12536.1"
                     /translation="MTSKTHKQHLTRPTQSMPNPTVAEYVIARLADLGIAHVFGLPGD
                     FSFPFDDAIEASDKLSWIVSSNELNAAYSADGYARIYGAAILTSTYAVGELSALNGVM
                     GAKAERLPVFHLVGAPSMRLARSRKSIHHSFGDGESGQFRQLSEMTACASTFLTPENT
                     ISEMERVISAALAQRQPAYITVPQDYALMPVVGKPIHGVPLPKVPTFSSDPRELATAL
                     KTIGARLSKAKFPVILPAFTISRYGLQKEVGRLLAATGIPFATTGMDKGVISESHPLY
                     LGMYVGDASTPAVRKIVERADLVLNLGGVLFTDFSTGLFSDHLQCSRIVTVWPDHVEV
                     GASSDETASQSRTFGPVHMKDVLAGLTKQASRHKTPSFPRPKPIPASGAPGAQVTNAS
                     VNSRIQQFLAPGDIVVVETGIASFTLSAVLLPNRAVYHNQALWGSIGWATPAAFGAAL
                     ADPSRRVVLVTGDGSHQLTATEIGVMGRYGVKPILLVLNNGTFGIEEFLERNKNCDYN
                     KLAPWQYQMIPEVMGCRDWFCTSVLTNAELASALQKAREHPGAAYIEVLLGPKPIAPT
                     SPAKLAAGYQSKPTKA"
BASE COUNT          644 a          944 c          908 g          652 t
ORIGIN      
        1 ctgacgacac cagcacgctc ttcatcgact actgcttcct gcgctgttcg cggacgcgct
       61 tgaatgatcc gtcgcagttc atcaagtcgg cttggattcg gccggaggac ctcgccgttt
      121 atgaagcgat ggggtatacg accttcaagc tgctcgaacg gggaattccg tcggcggagc
      181 tgctgcggcg cgtcaaagcc tacagtgagc ggcggttcga tggcaatctg gcggaactgc
      241 tgctgtcgta tggcttcaag caaccggtcc gcaaggaatc cctgtggagt ctgcgtcact
      301 tctggaagcc gcagcaggtg aatccgcggc gattgaaacc gctgctcgac ctggcacgcc
      361 tgcaaggctg gctctcgcct ctgccggagt gtccgatccg ggtcgaggcg cggaagatcc
      421 cggagaattt cctggacggt tttcgcgagc gtgactgcgc ctccttggac tgccagactt
      481 gcggctattg cgagcggatc gccgcgcagg ccgtttcgat cgcgtccgaa taccgctccg
      541 aggtgttgag aaagtacgcc gaaatggacg ataccatggt agcgggagct ctttggggtg
      601 tttgaatcac tcgctaccga agcggagttc ctggaccggc ccgactgtga cccggtacgg
      661 gccaccgcca gtggaaggtg tcaaaccagg agcaaactcg ccttgatctt tctgtcccct
      721 cgaaggatca tgcattggga ggccgagggc cggcttcgac gcactccgga ggacaccatt
      781 gaatggattg ggtttccggc ggccggccta tagctaagtg gcctgaattg ggctccgcct
      841 tcggaggggg gctccccccg cgacaccggt gggggtatcc ccaatagcta cagacgggcc
      901 tacgatgcac atttcctcca aatgaaaaca ttgttcacag ccgaagcgat ttccaaggga
      961 ggacgttcgg gaaccattca gacaccggac cggttgttgg atgttacgtt aggcaatccc
     1021 atggagaaag gaatcgagaa acgcggtccc aatcccgagc tgttgttcgc gggcgcatat
     1081 tccgcctgtt accacggtgc tctgggcaac gcggctaaaa agctcggtac gccggtcagg
     1141 gattccacgg tgcgggcact ggtgagcctg atcgaagagg atcaaggcgg ctaccggctg
     1201 gcggtggagt tgcatgcgca acttcccggg ctcgatcgcg cccaagctca acgcctcatg
     1261 gatgaagcgc acagaacttg cccctactcc agagccctgc gcggcgacac gtccgtcaag
     1321 ttggtggtgg attgaccagc aaaacccaca aacagcatct gactcgaccg acccaaagca
     1381 tgcccaatcc caccgtcgca gaatatgtca ttgctcgtct agctgatcta ggaattgccc
     1441 acgtctttgg tttgccgggt gatttctcat ttcctttcga cgatgccatc gaggcgagcg
     1501 acaagctctc ctggatcgtc tcgagcaacg aactgaacgc agcctattcc gcggacggtt
     1561 acgcccggat ttacggagca gccatactga cctccactta tgccgtgggg gaattgagcg
     1621 cgctgaacgg agttatgggg gccaaggcgg aacggttgcc tgtctttcat cttgtgggag
     1681 ccccgagtat gcggttggct cggtctcgca agtcgattca ccactccttt ggtgacggag
     1741 agtccggcca gttccgtcaa ctctccgaga tgacggcctg cgccagcaca tttctcactc
     1801 cggaaaacac gatctccgag atggagcgcg tgatttccgc ggcgctcgct cagcgccaac
     1861 ccgcttacat cacagtccca caggattacg ctctcatgcc agtcgtagga aaaccgatcc
     1921 acggcgttcc cctcccgaag gtgccgactt tttcgagcga tcctcgcgag ttggccacgg
     1981 ccttgaaaac gattggggcg cgactctcga aggcgaaatt cccggttatt ctccccgcct
     2041 tcacaatctc gcgctatgga ctacaaaagg aagtgggccg acttctggct gcgaccggaa
     2101 tcccctttgc caccactggc atggacaagg gcgtaatctc ggaatcccat cccctctatc
     2161 tcggaatgta tgtgggtgac gcctcgacgc cagcggttcg caaaatcgtg gaaagggcgg
     2221 atctcgtgct caatctcggc ggcgtgctat ttacggattt ttccaccggc ctgttctccg
     2281 atcaccttca atgttcccga atagtgactg tgtggccgga ccacgtggag gtcggcgcct
     2341 cttcggacga aaccgcttct caatccagga ccttcgggcc ggtccacatg aaagacgtac
     2401 tggcaggtct cacaaaacaa gcgtcgaggc acaagacgcc ctcgttccct cgtccaaagc
     2461 ctattcccgc ttcgggtgcg ccgggcgctc aggtcacgaa cgcgtcggtc aattcgagga
     2521 tccagcaatt tcttgcccca ggcgacatcg tggttgtgga aactggcatc gcttctttca
     2581 ccttgtccgc agttcttctg cccaacagag cggtgtacca caatcaagcc ctctggggct
     2641 cgatcggttg ggcaacgccg gccgcctttg gtgctgcgtt ggctgatccc tcacgccgtg
     2701 tcgttctggt cacgggagat ggatcccacc aactcacggc caccgagatt ggggtaatgg
     2761 ggcgatacgg ggtaaagccc attctgctgg tgctcaacaa cggcactttc ggcatcgaag
     2821 agtttctgga gcgaaacaaa aactgcgact acaacaaact tgctccctgg cagtaccaaa
     2881 tgattcctga agtcatgggt tgccgcgatt ggttttgcac gagcgtgtta acaaacgcgg
     2941 agctcgcgtc ggccctgcag aaggcaaggg aacatcccgg ggccgcatac atcgaggttc
     3001 tcctcggacc gaaaccgata gctcccacct cgcccgccaa gctcgcggcg ggctaccaaa
     3061 gcaagcccac caaagcgtag ttgcgccggc ctcaaaggcg gaggttaatt ccggaactca
     3121 gcctttggcg gaaaaatacc atctcaac
//
